LOCUS       DAZKIV010000001       102726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZKIV010000001 DAZKIV010000000
VERSION     DAZKIV010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 102726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 102726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..102726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.001"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..566
                     /gene="yfgH"
                     /locus_tag="ACHOT6_000001"
     CDS             48..566
                     /gene="yfgH"
                     /locus_tag="ACHOT6_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9945298.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            582..1121
                     /gene="yfgI"
                     /locus_tag="ACHOT6_000002"
     CDS             582..1121
                     /gene="yfgI"
                     /locus_tag="ACHOT6_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFP9945299.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="ACHOT6_000003"
     CDS             complement(1216..2793)
                     /gene="guaA"
                     /locus_tag="ACHOT6_000003"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFP9945300.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LSYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="ACHOT6_000004"
     CDS             complement(2862..4328)
                     /gene="guaB"
                     /locus_tag="ACHOT6_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFP9945301.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4490..5860
                     /gene="xseA"
                     /locus_tag="ACHOT6_000005"
     CDS             4490..5860
                     /gene="xseA"
                     /locus_tag="ACHOT6_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFP9945302.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5857..6072)
                     /gene="yfgJ"
                     /locus_tag="ACHOT6_000006"
     CDS             complement(5857..6072)
                     /gene="yfgJ"
                     /locus_tag="ACHOT6_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9945303.1"
                     /translation="MELHCPQCQHVLDQDNDHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6141..7613)
                     /gene="der"
                     /locus_tag="ACHOT6_000007"
     CDS             complement(6141..7613)
                     /gene="der"
                     /locus_tag="ACHOT6_000007"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFP9945304.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7731..8909)
                     /gene="bamB"
                     /locus_tag="ACHOT6_000008"
     CDS             complement(7731..8909)
                     /gene="bamB"
                     /locus_tag="ACHOT6_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFP9945305.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8920..9540)
                     /gene="yfgM"
                     /locus_tag="ACHOT6_000009"
     CDS             complement(8920..9540)
                     /gene="yfgM"
                     /locus_tag="ACHOT6_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFP9945306.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9558..10832)
                     /gene="hisS"
                     /locus_tag="ACHOT6_000010"
     CDS             complement(9558..10832)
                     /gene="hisS"
                     /locus_tag="ACHOT6_000010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFP9945307.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10943..12061)
                     /gene="ispG"
                     /locus_tag="ACHOT6_000011"
                     /gene_synonym="gcpE"
     CDS             complement(10943..12061)
                     /gene="ispG"
                     /locus_tag="ACHOT6_000011"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFP9945308.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAVGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12088..13101)
                     /gene="rodZ"
                     /locus_tag="ACHOT6_000012"
     CDS             complement(12088..13101)
                     /gene="rodZ"
                     /locus_tag="ACHOT6_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFP9945309.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13386..14540)
                     /gene="rlmN"
                     /locus_tag="ACHOT6_000013"
     CDS             complement(13386..14540)
                     /gene="rlmN"
                     /locus_tag="ACHOT6_000013"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFP9945310.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14690..15121)
                     /gene="ndk"
                     /locus_tag="ACHOT6_000014"
     CDS             complement(14690..15121)
                     /gene="ndk"
                     /locus_tag="ACHOT6_000014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFP9945311.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15270..17582)
                     /gene="pbpC"
                     /locus_tag="ACHOT6_000015"
     CDS             complement(15270..17582)
                     /gene="pbpC"
                     /locus_tag="ACHOT6_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFP9945312.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEVAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17583..22544)
                     /gene="yfhM"
                     /locus_tag="ACHOT6_000016"
     CDS             complement(17583..22544)
                     /gene="yfhM"
                     /locus_tag="ACHOT6_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFP9945313.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEDSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22751..23596
                     /gene="sseA"
                     /locus_tag="ACHOT6_000017"
     CDS             22751..23596
                     /gene="sseA"
                     /locus_tag="ACHOT6_000017"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFP9945314.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            23932..24057
                     /gene="timP"
                     /locus_tag="ACHOT6_000018"
     CDS             23932..24057
                     /gene="timP"
                     /locus_tag="ACHOT6_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFP9945315.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24089..24865)
                     /gene="sseB"
                     /locus_tag="ACHOT6_000019"
     CDS             complement(24089..24865)
                     /gene="sseB"
                     /locus_tag="ACHOT6_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFP9945316.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQAMGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25007..26290)
                     /gene="pepB"
                     /locus_tag="ACHOT6_000020"
     CDS             complement(25007..26290)
                     /gene="pepB"
                     /locus_tag="ACHOT6_000020"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFP9945317.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     AAQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26468..26668)
                     /gene="iscX"
                     /locus_tag="ACHOT6_000021"
     CDS             complement(26468..26668)
                     /gene="iscX"
                     /locus_tag="ACHOT6_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFP9945318.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26680..27015)
                     /gene="fdx"
                     /locus_tag="ACHOT6_000022"
     CDS             complement(26680..27015)
                     /gene="fdx"
                     /locus_tag="ACHOT6_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFP9945319.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27017..28867)
                     /gene="hscA"
                     /locus_tag="ACHOT6_000023"
     CDS             complement(27017..28867)
                     /gene="hscA"
                     /locus_tag="ACHOT6_000023"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFP9945320.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSDAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28884..29399)
                     /gene="hscB"
                     /locus_tag="ACHOT6_000024"
     CDS             complement(28884..29399)
                     /gene="hscB"
                     /locus_tag="ACHOT6_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFP9945321.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29495..29818)
                     /gene="iscA"
                     /locus_tag="ACHOT6_000025"
     CDS             complement(29495..29818)
                     /gene="iscA"
                     /locus_tag="ACHOT6_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFP9945322.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29835..30221)
                     /gene="iscU"
                     /locus_tag="ACHOT6_000026"
     CDS             complement(29835..30221)
                     /gene="iscU"
                     /locus_tag="ACHOT6_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFP9945323.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30249..31463)
                     /gene="iscS"
                     /locus_tag="ACHOT6_000027"
     CDS             complement(30249..31463)
                     /gene="iscS"
                     /locus_tag="ACHOT6_000027"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFP9945324.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31704..32192)
                     /gene="iscR"
                     /locus_tag="ACHOT6_000028"
     CDS             complement(31704..32192)
                     /gene="iscR"
                     /locus_tag="ACHOT6_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFP9945325.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32462..33202)
                     /gene="trmJ"
                     /locus_tag="ACHOT6_000029"
     CDS             complement(32462..33202)
                     /gene="trmJ"
                     /locus_tag="ACHOT6_000029"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFP9945326.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33321..34124
                     /gene="suhB"
                     /locus_tag="ACHOT6_000030"
     CDS             33321..34124
                     /gene="suhB"
                     /locus_tag="ACHOT6_000030"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFP9945327.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34269..35123
                     /gene="yfhR"
                     /locus_tag="ACHOT6_000031"
     CDS             34269..35123
                     /gene="yfhR"
                     /locus_tag="ACHOT6_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9945328.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35314..36594
                     /gene="csiE"
                     /locus_tag="ACHOT6_000032"
     CDS             35314..36594
                     /gene="csiE"
                     /locus_tag="ACHOT6_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFP9945329.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36586..37725)
                     /gene="hcaT"
                     /locus_tag="ACHOT6_000033"
     CDS             complement(36586..37725)
                     /gene="hcaT"
                     /locus_tag="ACHOT6_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFP9945330.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37885..38775)
                     /gene="hcaR"
                     /locus_tag="ACHOT6_000034"
     CDS             complement(37885..38775)
                     /gene="hcaR"
                     /locus_tag="ACHOT6_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFP9945331.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            38911..40272
                     /gene="hcaE"
                     /locus_tag="ACHOT6_000035"
     CDS             38911..40272
                     /gene="hcaE"
                     /locus_tag="ACHOT6_000035"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFP9945332.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40269..40787
                     /gene="hcaF"
                     /locus_tag="ACHOT6_000036"
     CDS             40269..40787
                     /gene="hcaF"
                     /locus_tag="ACHOT6_000036"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFP9945333.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40787..41107
                     /gene="hcaC"
                     /locus_tag="ACHOT6_000037"
     CDS             40787..41107
                     /gene="hcaC"
                     /locus_tag="ACHOT6_000037"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFP9945334.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41104..41916
                     /gene="hcaB"
                     /locus_tag="ACHOT6_000038"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41104..41916
                     /gene="hcaB"
                     /locus_tag="ACHOT6_000038"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFP9945335.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41926..43128
                     /gene="hcaD"
                     /locus_tag="ACHOT6_000039"
     CDS             41926..43128
                     /gene="hcaD"
                     /locus_tag="ACHOT6_000039"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFP9945336.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43225..43647
                     /gene="yphA"
                     /locus_tag="ACHOT6_000040"
     CDS             43225..43647
                     /gene="yphA"
                     /locus_tag="ACHOT6_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9945337.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43695..44567)
                     /gene="yphB"
                     /locus_tag="ACHOT6_000041"
     CDS             complement(43695..44567)
                     /gene="yphB"
                     /locus_tag="ACHOT6_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9945338.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLELEQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(44579..45673)
                     /gene="yphC"
                     /locus_tag="ACHOT6_000042"
     CDS             complement(44579..45673)
                     /gene="yphC"
                     /locus_tag="ACHOT6_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9945339.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDKVLEVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45706..46704)
                     /gene="yphD"
                     /locus_tag="ACHOT6_000043"
     CDS             complement(45706..46704)
                     /gene="yphD"
                     /locus_tag="ACHOT6_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9945340.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46729..48240)
                     /gene="yphE"
                     /locus_tag="ACHOT6_000044"
     CDS             complement(46729..48240)
                     /gene="yphE"
                     /locus_tag="ACHOT6_000044"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9945341.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVRQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYASSQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48263..49246)
                     /gene="yphF"
                     /locus_tag="ACHOT6_000045"
     CDS             complement(48263..49246)
                     /gene="yphF"
                     /locus_tag="ACHOT6_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFP9945342.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49343..52624)
                     /gene="yphG"
                     /locus_tag="ACHOT6_000046"
     CDS             complement(49343..52624)
                     /gene="yphG"
                     /locus_tag="ACHOT6_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFP9945343.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPERAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDYQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52742..53935
                     /gene="yphH"
                     /locus_tag="ACHOT6_000047"
     CDS             52742..53935
                     /gene="yphH"
                     /locus_tag="ACHOT6_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFP9945344.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVYDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53999..55252)
                     /gene="glyA"
                     /locus_tag="ACHOT6_000048"
     CDS             complement(53999..55252)
                     /gene="glyA"
                     /locus_tag="ACHOT6_000048"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFP9945345.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55580..56770
                     /gene="hmpA"
                     /locus_tag="ACHOT6_000049"
     CDS             55580..56770
                     /gene="hmpA"
                     /locus_tag="ACHOT6_000049"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFP9945346.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56815..57153)
                     /gene="glnB"
                     /locus_tag="ACHOT6_000050"
     CDS             complement(56815..57153)
                     /gene="glnB"
                     /locus_tag="ACHOT6_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFP9945347.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57214..58548)
                     /gene="glrR"
                     /locus_tag="ACHOT6_000051"
     CDS             complement(57214..58548)
                     /gene="glrR"
                     /locus_tag="ACHOT6_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFP9945348.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58538..59251)
                     /gene="qseG"
                     /locus_tag="ACHOT6_000052"
     CDS             complement(58538..59251)
                     /gene="qseG"
                     /locus_tag="ACHOT6_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFP9945349.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59416..60843)
                     /gene="qseE"
                     /locus_tag="ACHOT6_000053"
     CDS             complement(59416..60843)
                     /gene="qseE"
                     /locus_tag="ACHOT6_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFP9945350.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61419..65306)
                     /gene="purL"
                     /locus_tag="ACHOT6_000054"
                     /gene_synonym="purI"
     CDS             complement(61419..65306)
                     /gene="purL"
                     /locus_tag="ACHOT6_000054"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFP9945351.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIGSLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APFIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLAGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65564..67120
                     /gene="mltF"
                     /locus_tag="ACHOT6_000055"
     CDS             65564..67120
                     /gene="mltF"
                     /locus_tag="ACHOT6_000055"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFP9945352.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67117..67620)
                     /gene="tadA"
                     /locus_tag="ACHOT6_000056"
     CDS             complement(67117..67620)
                     /gene="tadA"
                     /locus_tag="ACHOT6_000056"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFP9945353.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67678..68313)
                     /gene="yfhb"
                     /locus_tag="ACHOT6_000057"
     CDS             complement(67678..68313)
                     /gene="yfhb"
                     /locus_tag="ACHOT6_000057"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFP9945354.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68522..69370
                     /gene="yfhH"
                     /locus_tag="ACHOT6_000058"
     CDS             68522..69370
                     /gene="yfhH"
                     /locus_tag="ACHOT6_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFP9945355.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69684..69950
                     /locus_tag="ACHOT6_000059"
     CDS             69684..69950
                     /locus_tag="ACHOT6_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945356.1"
                     /translation="MRRKESNVSSLPELTNFEVSYSLVTNEVYLSASFTDNMACIPNW
                     PLQEFPDQLICISRAKAVALIEELQKTINYMDAGIDRSSGSLLQ"
     gene            70011..70658
                     /locus_tag="ACHOT6_000060"
     CDS             70011..70658
                     /locus_tag="ACHOT6_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945357.1"
                     /translation="MSEFSNLLNSIPGWLSSSLTALVGTLIGGWFTLKGVTQQAKLSK
                     VETERESLELQLSVLKGVKGEVFTLINLYNKRMKIHIDNIKPGQMLILTFPVGDDNFT
                     FYEQNANVIAKLNDSARDSIINIYTYSRSLIQSFKGNNKLIEDYEKILIGMADNNKDK
                     TMYKRLHDAKIDVMVDYAQGIKNIDAELRDAVNKGFNIIDQEVKSLQMKLNKLAS"
     gene            complement(70739..70864)
                     /locus_tag="ACHOT6_000061"
                     /pseudo
     CDS             complement(70739..70864)
                     /locus_tag="ACHOT6_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            71020..71592
                     /locus_tag="ACHOT6_000062"
     CDS             71020..71592
                     /locus_tag="ACHOT6_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HFP9945358.1"
                     /translation="MLSYLIAIHFVLLGNSSDLKSFWKSEVIRREYMDIWRLFREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNNKIINKFGCEIGLGAKIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDIPGSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(71636..72214)
                     /locus_tag="ACHOT6_000063"
     CDS             complement(71636..72214)
                     /locus_tag="ACHOT6_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9945359.1"
                     /translation="MAFRMSEQSRTIKIYNLLAGTNEFIGDGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNTDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTSWVKDAEAEKLFRSQEAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVDTTVAADIEWPVAPQ"
     gene            complement(72214..72837)
                     /locus_tag="ACHOT6_000064"
                     /pseudo
     CDS             complement(72214..72837)
                     /locus_tag="ACHOT6_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(73290..73970)
                     /locus_tag="ACHOT6_000065"
     CDS             complement(73290..73970)
                     /locus_tag="ACHOT6_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HFP9945360.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWVFPDT
                     GISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGVLL"
     gene            complement(73967..75166)
                     /locus_tag="ACHOT6_000066"
     CDS             complement(73967..75166)
                     /locus_tag="ACHOT6_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFP9945361.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMVTATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGTPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTIPDKYGNPHVIKFSRSSDVPVYARIKLQVFTGYTSQIGQQIQQAISDYINSLMIG
                     DSVLLSRIYSPANLGVVSGGNARYYDIQELTIGKSPGALSSSNIDIRYNESVSCTPEN
                     IVITVES"
     gene            complement(75166..75519)
                     /locus_tag="ACHOT6_000067"
     CDS             complement(75166..75519)
                     /locus_tag="ACHOT6_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945362.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGRQKPETYNLAIRKRILETQGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            complement(75519..76271)
                     /locus_tag="ACHOT6_000068"
     CDS             complement(75519..76271)
                     /locus_tag="ACHOT6_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gp138 family membrane-puncturing spike protein"
                     /protein_id="HFP9945363.1"
                     /translation="MALSDQTRSGDLAETFKSERDTTKNQIRVALPGIVQSFDPDAVT
                     AVVQPAIRSVETDNDGNRVTKNYPLLVDVPVIFPRGGGCTLTFPVEAGDECLVIFADR
                     CIDFWWQNGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTGKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGAVE
                     FLGSVKANGKPIDDTHTYGGVQRGGSSTDGVN"
     gene            complement(76335..76757)
                     /locus_tag="ACHOT6_000069"
     CDS             complement(76335..76757)
                     /locus_tag="ACHOT6_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945364.1"
                     /translation="MPVAINCFLEMAKSSIENSGEQWTRNAISRAYYTMYHSALLLTD
                     GYIPTQDEKGQKLPGGVHARLSEYLCGENAALAHSLDRTSAKKVGLKLKTAHHRRVIA
                     DYNLDKHVNRIDACSTIKDAEDLQSLIAGMIDTKKTAS"
     gene            complement(76757..77488)
                     /locus_tag="ACHOT6_000070"
     CDS             complement(76757..77488)
                     /locus_tag="ACHOT6_000070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945365.1"
                     /translation="MPVAQPKTGEVVAKLGPSLEAGRILLLDLEKQKLLRDARALPIR
                     YQSLALEGLILLLDGKVERGIEAIETSLAICPTDPVTWNNYSSALSNLGLYSKRRELL
                     TRALDYDFPCMVEHALNFAAFWADGEILDMAIAKLEKFGQTEKEKHVSALETAVVLSN
                     LGGPMSDGLKKAADVLMYIVESESLRAKTSSLLTDGEGYGSFSVGIETEDAEYLSYLN
                     DKVVDEMIERGLETGCSVAYFEAVN"
     gene            complement(77625..77966)
                     /locus_tag="ACHOT6_000071"
     CDS             complement(77625..77966)
                     /locus_tag="ACHOT6_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945366.1"
                     /translation="MRGIIFLLAVFSACSAWADGFTVKCGGYTMVANQGELSTINGER
                     VTSQKITELGTNGLKVDMVLMPAKDGNNYGFEYIRRPGTEKRFLNVQLLQNSMDAPKI
                     IGSFPCKKVYG"
     gene            complement(77970..79031)
                     /locus_tag="ACHOT6_000072"
     CDS             complement(77970..79031)
                     /locus_tag="ACHOT6_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945367.1"
                     /translation="MSQNWMRHFELQLVDGNGQGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVSTSDVGTVREVDAADVGQSDGR
                     NYGLIFSGEIRYSVTGKDSPVDSYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVFPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHKAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTTAQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAADLYTQSALQRG"
     gene            complement(79034..79336)
                     /locus_tag="ACHOT6_000073"
     CDS             complement(79034..79336)
                     /locus_tag="ACHOT6_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945368.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDLYWIMDLLNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLLVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            complement(79336..79923)
                     /locus_tag="ACHOT6_000074"
     CDS             complement(79336..79923)
                     /locus_tag="ACHOT6_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFP9945369.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVIISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            complement(79923..81911)
                     /locus_tag="ACHOT6_000075"
     CDS             complement(79923..81911)
                     /locus_tag="ACHOT6_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HFP9945370.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYWASQRTGATVHGIQSIGYAVSQVGGSADAARGSLESL
                     ARFIRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSGMPYYRANQYAQMLG
                     IDENTLMAMRRGVGGFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILALGDIIGRLFFRLIEGTSS
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKTTVNDLAKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVSIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQHEQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSQELINKAAVAGISGKVTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVS"
     gene            complement(81901..82077)
                     /locus_tag="ACHOT6_000076"
     CDS             complement(81901..82077)
                     /locus_tag="ACHOT6_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP pyrophosphohydrolase"
                     /protein_id="HFP9945371.1"
                     /translation="MPDGLSYLLNPVDAGLIPYTALKDGSVDLYDIALLNDHLAVKAD
                     NQRRIEKWREDNER"
     gene            complement(82089..82541)
                     /locus_tag="ACHOT6_000077"
     CDS             complement(82089..82541)
                     /locus_tag="ACHOT6_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9945372.1"
                     /translation="MEFEIKGVKYRAAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTDAIIHPCLAVVSRRHMDGWV
                     PVFTQGELMFDDIDLLVMLQLVARVVANSLGNFLPTSLTSTTQSLQQG"
     gene            complement(82545..82985)
                     /locus_tag="ACHOT6_000078"
     CDS             complement(82545..82985)
                     /locus_tag="ACHOT6_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HFP9945373.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGADGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            complement(82996..84141)
                     /locus_tag="ACHOT6_000079"
     CDS             complement(82996..84141)
                     /locus_tag="ACHOT6_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 family protein"
                     /protein_id="HFP9945374.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDEDIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSSVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            complement(84145..84708)
                     /locus_tag="ACHOT6_000080"
     CDS             complement(84145..84708)
                     /locus_tag="ACHOT6_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945375.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPAETNWCAFGVIGWSGDDSPAFTRQTDDGSQLWRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     TVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            complement(84683..85072)
                     /locus_tag="ACHOT6_000081"
     CDS             complement(84683..85072)
                     /locus_tag="ACHOT6_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HFP9945376.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDEDGIGSDSEVKKQF
                     AGVVTVDRSLENRRMQAGQVISGAILIVTTERLTQGQTGRDSDIVTYQGRDYRVTFVD
                     PYTAYGAGFVQAHCELLPFDGGIPVEQ"
     gene            complement(85059..85613)
                     /locus_tag="ACHOT6_000082"
     CDS             complement(85059..85613)
                     /locus_tag="ACHOT6_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945377.1"
                     /translation="MSFKSGVTTRVDNAKAILDALRSLTKKDVLVGIPSEDSGRDDVP
                     FGNAGIGYLNEYGSPEQNIPPRPHLVPGVKSAEEQTVPQLKAAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGATLELARRAAGESPGTDLA
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            complement(85610..86017)
                     /locus_tag="ACHOT6_000083"
     CDS             complement(85610..86017)
                     /locus_tag="ACHOT6_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HFP9945378.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDIS
                     ATLNPDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            86016..86249
                     /locus_tag="ACHOT6_000084"
                     /pseudo
     CDS             86016..86249
                     /locus_tag="ACHOT6_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(86246..87187)
                     /locus_tag="ACHOT6_000085"
     CDS             complement(86246..87187)
                     /locus_tag="ACHOT6_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HFP9945379.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTGTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGDKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(87199..87705)
                     /locus_tag="ACHOT6_000086"
     CDS             complement(87199..87705)
                     /locus_tag="ACHOT6_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945380.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGVYVRPYPTTSQPDMVRQVGSGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMATSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
     gene            complement(87709..88929)
                     /locus_tag="ACHOT6_000087"
     CDS             complement(87709..88929)
                     /locus_tag="ACHOT6_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HFP9945381.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAGQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKSWWTRMRTAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRPNIGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(88944..89678)
                     /locus_tag="ACHOT6_000088"
     CDS             complement(88944..89678)
                     /locus_tag="ACHOT6_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor head protein"
                     /protein_id="HFP9945382.1"
                     /translation="MTNRRNRQAEIRYRTSLRRIARAVGDIVNGHYDGSNDSITEIIE
                     ALERYSEIITPWATKVAENFTADIVRKNDEQWRKHSKTISRELRNLVNSAPPGQVMKS
                     IVAEQVKYIKSLPLEAADRVYDIQNRAIEAVVTGGRAEHFAKEIAASGDIAKSRADLI
                     ARTELGRATGALDQARALAIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDGM
                     TGHAGELPNCRCYKEIVFPTSHSYPA"
     gene            complement(89569..91035)
                     /locus_tag="ACHOT6_000089"
     CDS             complement(89569..91035)
                     /locus_tag="ACHOT6_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HFP9945383.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDCGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMIDPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSLFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSAGGRGHRKWSLRWFK"
     gene            complement(91035..92657)
                     /locus_tag="ACHOT6_000090"
     CDS             complement(91035..92657)
                     /locus_tag="ACHOT6_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HFP9945384.1"
                     /translation="MPLPFPFDFKHPDYQMVFEWRMERLQRIRQNPEILPVLKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERWGNRENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPVEFR
                     GSWSEKKHAPYMRVEFPETGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NELRNAARRPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            complement(92660..93232)
                     /locus_tag="ACHOT6_000091"
     CDS             complement(92660..93232)
                     /locus_tag="ACHOT6_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9945385.1"
                     /translation="MTKKLKAKHEVFCREFLVDLNATQAAIRAGYVSRRAHVTGAELY
                     GKPEIRARINELKQERIDQLGIDANYVLMRLVEIDRLDVADILEDDLSIKPLSAWPES
                     WRRYLSGFNLAEMFEGRGDDREMVGILKKIKWPDKVKNLELLGRHVSVQAFKDNVKNE
                     VTGADGGPVRTEITNLTPEQAAEAYRKMMG"
     gene            complement(93294..93818)
                     /locus_tag="ACHOT6_000092"
     CDS             complement(93294..93818)
                     /locus_tag="ACHOT6_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945386.1"
                     /translation="MKLTYKIVIAAFFFSAFGALVWSANHYHSKYQAEKLRADKAEGE
                     AEYQGKVIANQALNFNRFNQIAENASRLNSLVDIGHEKTVIKYREVLLREKNCDFPVP
                     VDIAVGLLNYANRLRASALHADSGDIDSAGDRATTTRTLTYCQAVLWINPLLAAIEKA
                     NNQLAGVRQIEQSR"
     gene            complement(93802..94278)
                     /locus_tag="ACHOT6_000093"
     CDS             complement(93802..94278)
                     /locus_tag="ACHOT6_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFP9945387.1"
                     /translation="MQTLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYCKQLGLKDFSPESQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLISRFKDAGGVVNEADL"
     gene            complement(94282..94623)
                     /locus_tag="ACHOT6_000094"
     CDS             complement(94282..94623)
                     /locus_tag="ACHOT6_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFP9945388.1"
                     /translation="MKMHNAPHSWPDLLELLQSWWRGDTPLGAVVMSIVMAGLRIAYF
                     GGGGGWKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMR
                     VIGNKFGGSKE"
     gene            complement(95027..95461)
                     /locus_tag="ACHOT6_000095"
     CDS             complement(95027..95461)
                     /locus_tag="ACHOT6_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9945389.1"
                     /translation="MRNIQQVLERWGGWAAASNTTVSWAPIAAGFKGLVVSSSPGRLS
                     CCDDDGLIIDACVCRLQQVRKPEELDVIMLYYVYGLSKREIGRRRRCSEGFIRQQLQV
                     AEGFIEGCLCMLGVSLQMDPEVEIQRDEKSVSALRKNCATLV"
     gene            complement(95748..97934)
                     /locus_tag="ACHOT6_000096"
     CDS             complement(95748..97934)
                     /locus_tag="ACHOT6_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapE domain-containing protein"
                     /protein_id="HFP9945390.1"
                     /translation="MSDSKPWGATPDEWFHFDLVLGRTAHLLPVVCNPGATISPDSKL
                     KALGKTPSRYNRDRQVTGIAQWTGYVVTEHDFARWSNEPDYGICVRTGHGWLALDCDS
                     EDEDIQADIRKTLVQLLGESPPRRWRANSNKCLYLLAVDGDFRKRIHRLAGDMGIIEL
                     LANGQQFVACGTHSSGARIEWDGGLPDEPPAITGEQLETLWQRLAEQLPVSVTTEAGN
                     TKMRDRSAFTPGATDDTAEYLDANGWTLLDGANGERYIRCPFEDGHSSGGDPTSTVYF
                     PAGTAGFEQGHFKCLHASCAHRDDGDFLNAIGIRNDDFEDLTSTEVAEPLPLPAFERD
                     KWGRIEATISNAAKAVVRPDFVDIDIRFDQFRDEIMFAPAGSGQWQAFTDADYARLRI
                     TMEKRGFKPVGRELIRDVVLLAADEQPFDSAITWLNGLEWDGVPRIECFYHTHFGTAD
                     TPYTRAVSMYMWTALAGRVLEPGIKADMVPILVGPQGCGKSSGVEALSPDPAFFTEIS
                     FAEKDDDLARKMRGRLVAEIGELRGLNTKELESIKAFVTRTHENWIPKYREFATQFPR
                     RLVFVGTTNEDEFLADKTGNRRWLPVEVSKVDVKAIKRDLLLLWAEAREVFQRLGGIQ
                     FREAEQLAASVHEQYTIKDAWLETVEKWLDTPDLMTNELPRNCEFLRASDVLRDAIGL
                     NPDRIGKREEMRISNVLQNCGYKRAQRRIGGKKCKVWEPLEPRGTT"
     gene            complement(97927..98109)
                     /locus_tag="ACHOT6_000097"
     CDS             complement(97927..98109)
                     /locus_tag="ACHOT6_000097"
                     /inference="COORDINATES: protein motif:HMM:NF025900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9945391.1"
                     /translation="MKKAEAIELAGSKAKLARLLKVSKGAVSQWGEEIPELRALQLEK
                     ILEQKNVVKQKGLTHV"
     gene            98223..98915
                     /locus_tag="ACHOT6_000098"
     CDS             98223..98915
                     /locus_tag="ACHOT6_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9945392.1"
                     /translation="MINDRIREARRNVHLTQDALAKRIGLTKATISQWESGNTTPNGK
                     NLINLAAALNVSPEWLLSGKTSDTKEIHSNAQVEGGFSVWDSSTPLEDDEVEIPFYQE
                     IELSAGNGTYVDIDRMGCKLRFARSTLRKAGVDVNCAACVSIHGNSMEPVLPDGAVVG
                     IDTSKTGVKDGQMYAIDQDGLLRVKLLYRLPSGLRIRSFNRDEYADEEYFNQEANKIR
                     IIGQVFWYSVLL"
     gene            99549..99698
                     /locus_tag="ACHOT6_000099"
     CDS             99549..99698
                     /locus_tag="ACHOT6_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945393.1"
                     /translation="MKPEHIHRLTGRDVLRYRRKNFDLMTGLAVVTALGLIITFILLV
                     ARTTV"
     gene            99695..100597
                     /locus_tag="ACHOT6_000100"
     CDS             99695..100597
                     /locus_tag="ACHOT6_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945394.1"
                     /translation="MSLETSLELNNQLLAQHNALLERLIRTMASGIVMHPDTISRVQE
                     CRDTATETENMSAAMTLDDLEFSDVIALAGFYPVATPITEDMLQRAVAYRDAEGDKRV
                     VQIDALDSALQGVKRAKALHKSALLDLSRNILKFWDDLPTIGERRAFAEQLLDAPADG
                     RDEVKPKKASNKDGERTGPFYVKNVSGTAASELHTLRKLNEMLKKGHIEINRVEYLQL
                     QEEFARRDAANSSQNNDAKDDHTIDFAALRKQAEGLILQLAKGGYRAEAIAILEKQGA
                     RKLGEVTDENLAEVITLAEKALEG"
     gene            100600..101901
                     /locus_tag="ACHOT6_000101"
     CDS             100600..101901
                     /locus_tag="ACHOT6_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HFP9945395.1"
                     /translation="MPDVHAQLSPSSAHRWMRCPGSLALEATQPDKETTFAIEGTAAH
                     ALAEKVLRNRQSHPEHYAGCNVSMFLGSYPLRENPDDTSGPQVDDEMVDAVGRYVDTV
                     WTLAQNNELLVEQRVDFSHITGVEESFGTADGIIIAGNELQIHDLKYGKGVRIDAEQN
                     EQLQLYALGALEQFSMLYDFETVRLFIHQPRLNHVSEWSLTVQELQSFGERAQKAATS
                     AILVLNIAECEGIETLPLENFIPGEKQCRFCKAKAICTAQKMQHLQTAASDFEDLTKP
                     VSEIITNASARVPLLTIEQLAEIYSQADFIESWLKAVRDRVHNELNAGHPVPGFKLVT
                     GKQGNRAWSDEEAARALLKDQFRYKTEEVFDFKLISPTKAEKLIKKASPRRWSKVEAL
                     ITRADGKPTVVPESDPRPALNINPVNDFDDVSDDTLTADLI"
     gene            101917..102465
                     /locus_tag="ACHOT6_000102"
     CDS             101917..102465
                     /locus_tag="ACHOT6_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HFP9945396.1"
                     /translation="MKLKLNNVRLAFPSLFEAKTVNGEGDPRFSAVFLMSPKHPQLEE
                     IRKAMKQVAKEKWGEKWESIYNQLEKKLNLCLHDGDEKAEYEGFPSNFFLNAANKARP
                     AVLDRDRSPLIQADGRPYAGCYVNAVIDIWAQDNNFGKRINASLGGVQFLRDGDAFAG
                     GGVASADDFDDISEGADAEALI"
     gene            complement(102492..>102726)
                     /locus_tag="ACHOT6_000103"
     CDS             complement(102492..>102726)
                     /locus_tag="ACHOT6_000103"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945397.1"
                     /translation="RYRLHSKIAAIFYLGYGLIFLCLLLNKGTVYMVLGVVVMLFCLF
                     TFFMFWTLFPGREVINKYNLQLLKVDASKYQVK"
BASE COUNT        24856 a        28028 c        25187 g        24655 t
ORIGIN      
        1 gatgatctta aatgtcttat ttttcgtaat gtgtataaca aggaatagtg atgaaattta
       61 aaaaatgtct tctgcctgtg gcaatgttag cgtcattcac tctggcagga tgccagtcaa
      121 atgctgacga tcatgccgcc gatgtttatc aaaccgatca actgaatacc aaacaagaaa
      181 ctaaaaccgt taatattatt tccattcttc ccgcaaaagt tgccgtagac aactcccaaa
      241 ataaacggaa cgcacaagcc ttcggcgcgc ttattggcgc agtcgctggc ggtgttatcg
      301 gccacaacgt cggttctggc agcaattccg gaacgacggc aggggcagtt ggcggcggag
      361 ctgtaggcgc ggcagcgggt tctatggtga atgataaaac cttagtggaa ggtgtttctt
      421 taacatataa ggaaggcacc aaagtgtata cctctaccca ggtgggtaaa gagtgccagt
      481 ttacgacagg tttagccgtt gttattacca caacgtataa cgaaacgcgt attcagccaa
      541 atactaaatg tcctgaaaag agctaataat caggaggagt catgaagaaa gtttttcttt
      601 gcgccatctt ggcctcctta agctatccgg ctatcgcctc atcattgcag gatcaactct
      661 ctgctgtagc agaagctgaa cagcaaggta aaaatgaaga gcaaaggcag catgacgaat
      721 gggtcgcgga gcgcaacagg gaaatccagc aagagaagca acgtcgcgca aatgcccagg
      781 ccgccgctaa caaaagagcg gcaacggcag cagcaaataa gaaagctcgt caggataaac
      841 tggacgccga agcctctgcg gacaaaaaac gcgatcaaag ttatgaagat gagctacgca
      901 gcttagagat tcagaaacaa aaactggcgc tggcgaaaga agaagcccgc gttaagcgag
      961 aaaacgaatt tatcgatcag gaactgaagc acaaagctgc gcaaaccgat gtggtgcaat
     1021 ctgaagctga cgccaacaga aatatgactg aaggcggtcg cgatctgatg aaaagcgtgg
     1081 gcaaagcaga agagaacaaa tcggacagct ggtttaatta atcgatgtta gtaacttcaa
     1141 gcctatgatt cttgaagata aaaaaccctc tgtagtaaca gagggttttg ttcattcata
     1201 gtgcagggat taaaatcatt cccactcaat ggtagctggc ggcttgccgc tgatgtcata
     1261 caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc gaccgaggaa
     1321 atcgtacggc agatgcgccc agtgtgcggt cataaagtcg atggtttcga cagcacgcag
     1381 agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac gtactggcag
     1441 gaagacggtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca gttcttcaat
     1501 gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt cacccagaac
     1561 acgaacgcca aggcctggtc ccgggaacgg atgacggtac agcatgtcgt acgacaggcc
     1621 cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg gttcaaccag
     1681 gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga tgacgtgtgc
     1741 tttaccggtt gcagaagccg cagattcgat aacgtcaggg tagatggtgc cctgcgccag
     1801 ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga aaacgcgccc
     1861 gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca ggaagcgatc
     1921 ttctgccggt acgtgaacaa tgttcagacc aaagtgatcg ccaaacatat ccagaacctg
     1981 ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca ggtttttacc
     2041 gatagcgcgg tgcagcagca ttgcggttac ggaggaatcc acaccaccag agaggccgag
     2101 gatgacttta tcgtcgccta cctgctcgcg gatgcgagct acagcatcgt cgataatttt
     2161 cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct ccagcatgcg
     2221 cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga agcgtttttc
     2281 ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga cgaagtcgga
     2341 cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg gtttaccgtc
     2401 tgcggtcagc gcatcttcga taccgcgaac cagtgcgctg tcgtttacga cttcaacctg
     2461 cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact gcattgccat
     2521 ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga catactgcgg
     2581 cgcacgcggg ctgttttctt cggtggtgct ttccgggccg ccggaaagaa taatgccgct
     2641 tggattgaag tcacgaattt gtgcttctgt cacatcccac gcccacagtt cgcagtaaac
     2701 acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga agtccagaat
     2761 gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg caagtgaaac
     2821 agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc cagacggtag
     2881 ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat gcccgcaccg
     2941 ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca gccggtcaga
     3001 cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag gcgaccttta
     3061 taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt atcgctctgg
     3121 aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc acggtaagat
     3181 ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt acccgccagc
     3241 atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat gtcgccggag
     3301 aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc ttcaactgcg
     3361 tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt cgtacagata
     3421 gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccaa agcgcgtgca
     3481 cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt agcacgggtt
     3541 tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat cagcagaacg
     3601 tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc tgcgccaact
     3661 gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt acgttccgct
     3721 ttctggaagt ctttcacggt gatcatgccg atcaggtgga attcgtcatc aaccaccagc
     3781 gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc ttcaccttca
     3841 cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg gttcaggtcg
     3901 gtaacaaaac gcacgtcacg accggtgata atacccacca gttcgttttc ttcggtaacg
     3961 accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg cagcgtcgtg
     4021 gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt cacacggcga
     4081 acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat accgccttcc
     4141 tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc ggaaagcata
     4201 gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt attcggcaga
     4261 acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc tttagcgata
     4321 cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg cattatacag
     4381 agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc cgaagggcgg
     4441 gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca tgttaccttc
     4501 tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc tgctgcttga
     4561 gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac aaccggcttc
     4621 cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg cgatgttccg
     4681 caacagcaac cgccgggtga ccttccgtcc acagcatgga caacaagttt tagttcgcgc
     4741 caatattacg ctctacgagc cgcgcggtga ctaccagata atcgttgaga gtatgcagcc
     4801 ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt tgcaggctga
     4861 aggtttgttc gatcagcaat acaaaaaacc acttccctcc cctgcgcatt gcgttggtgt
     4921 gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa aacgtcgcga
     4981 tccttctcta ccggtgatca tctaccccac cgccgttcag ggcgatgacg cgccggggca
     5041 aattgttcgc gccattgaac tggcgaatca gcgcaatgag tgcgacgtgt tgatcgttgg
     5101 gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg tagcgcgggc
     5161 tatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg atgtgaccat
     5221 tgccgatttt gttgccgatc tgcgtgcgcc aacgccgtct gccgccgctg aagtagtaag
     5281 ccgtaatcag caagagttac tgcgccaggt gcaatcggcc cgtcaacggc tggagatggc
     5341 gatggattat tatctcgcca accgtacgcg tcgctttacg cagattcatc atcgcttgca
     5401 gcagcagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgaac gcctgcaaaa
     5461 acggatgagc tttgcgctgg aaaatcagct taagcgtgcc gggcaacagc agcagcgatt
     5521 aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcacgc aaacgcgcat
     5581 tcagcaactg gaatatcgtt tagcagaaac cctgcgcgta cagcttagcg ccacgcgtga
     5641 acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa cgctggcgcg
     5701 tggatacagc gttactactg ctactgacgg caatgtactg aaaaaagtga agcaagttaa
     5761 agcgggtgaa atgctaacca cacgtctgga agacggctgg atagaaagtg aagttaaaaa
     5821 catccagccg gtaaaaaaat cgcgtaaaaa agtgcattaa gccagcacga attcaacgcg
     5881 ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctactgcac cgcaggcttt
     5941 taacacctgc aacggttgat ggcagtctgg acaaagagct ttcatttcaa taatttcccc
     6001 gcaggatggg caacgcgcat ggtcgttatc ctgatcaagc acatgctgac attgtggaca
     6061 atgcagttcc atatgaattc ctccggtagc catccctcta cattcataga gggaatggca
     6121 gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag acgcttacgt
     6181 ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt ttccccttct
     6241 ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa gtagttcatc
     6301 aagtagcgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat caccacaatc
     6361 ggagggttat aaccaccagc gtgggcatat ttcagcttca cacgacgacc gcgtaccagc
     6421 ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga ggtccccaca
     6481 cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc aacaccactg
     6541 ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag acggaagtcc
     6601 agcgtttctt tcacctgctc tttcacttcc tggctcaggc catcccactt attcaccaca
     6661 atgacaagtg agcgcccact attgagaata aagcccagca gcgagagatc ctgatcggaa
     6721 ataccttcgc gcgcatcaat cactaacatc accacgttgg cgtcttcaat ggcctgcaac
     6781 gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt acgtacgcca
     6841 gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttgggatgta gatgctgtca
     6901 cgcgtcgtgc caggcatgtc ataaacaaca acgcgctctt caccaagaat acggttagtg
     6961 agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg cagactttgc
     7021 gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa ttgcgcccag
     7081 tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca cggcagcagc
     7141 acatgctcca gcagacttaa tacgccacga ccgtgagacg cggcgatcgg gtagatttca
     7201 cctaagccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc gtcagttttg
     7261 tttgccacca ggaaggtcgg tttttcacgg gagcgcagat gtttggcaat cgcttcatct
     7321 gccggcatca agcccgcgcg cgcatccacc ataaacagta cgacgtccgc ttcttcaatc
     7381 gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc atcaatcccg
     7441 ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta cttacggtca
     7501 cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag acggttaaat
     7561 aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac catgtttaaa
     7621 gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg ttaaaaacag
     7681 gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga ttaacgtgta
     7741 atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc ggcaaccggt
     7801 tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc atcttcgacg
     7861 ttaatccagt gcagataacc ttcactgtca ccgaccacca ggttgccatt atacagcacc
     7921 ggagaagtca gcaggcgatg caacagatcg ctttgtgtcc acagcgtaac gccgccatca
     7981 atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt gccgtcgacg
     8041 atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact gcgcagatca
     8101 agcgccgtca ggttaccatt ataggccagc gcgaaaacaa cgccgttaac aacgacggga
     8161 gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcctg ggaaatacgc
     8221 tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc attatcgcca
     8281 cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa agagagcgaa
     8341 ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag cgcttgtaac
     8401 tgaccgttac tggtgtggat taacaccaga ccgtcgctga ccaccgggcg cgaaagtgct
     8461 tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag cgcgtaaacc
     8521 tgcgcctttt cgctgccaat gtagacatgc ccaccagaca cggtcacgcc gccagaaagt
     8581 aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga ccagatttct
     8641 ttgccatcat ccgcattcag cgcttttacc aaaccagcgc ggtccgctgc atagacaacg
     8701 ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct accaacggaa
     8761 gtactccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg ggacatcttt
     8821 accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac ggaaagcagt
     8881 cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca aattattaat
     8941 tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc ctgcttccca
     9001 tgcactacgc gcaccttgct tatcaccttt gctcagcaat gcttcaccac gcaggtcggc
     9061 aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg cggcatcagc
     9121 ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt tgagattttc
     9181 atcgctcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta gttcattttt
     9241 gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat ttttattttc
     9301 agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca ctgcggtaac
     9361 cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc tgttccagta
     9421 gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg ctttgccatt
     9481 ttcagcaaaa aagcgtttaa ccgcttctac ctggtcgttt tcgttctcgt aaatttccac
     9541 gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac gctatcctgc
     9601 gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc gttagccact
     9661 tcagactcac ccagcaccac agcaacgcgg gccccccatt tatcagcacg ggcaaactgt
     9721 ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgcccggtaa ttcatcacgc
     9781 agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc caccaggtat
     9841 atatcgacaa caggatcggc tttaaattcc ggattaacgg cctgtactaa caatacaaga
     9901 cgttcgaggc ccatcgcaaa accgacagcc ggtgttgcac gaccgcccag ttgttccaca
     9961 agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgagact gttagtcacc
    10021 cactcgaaaa cggtacggtt atagtaatcc agaccacgca ccagacgctg gtttacggtg
    10081 taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc acgagattcc
    10141 tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac ttccggattt
    10201 tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc ttcgtccagc
    10261 ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc gcgtgcttcc
    10321 agcgaaccga tagaattcag ctcaagagtt acgtgttcgg aaatacccag cgcgcgccac
    10381 cagcgggcgg tgagcataat cagttcagcg tcgatatccg gaccttgcag accgaaaact
    10441 tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc gtgacggaac
    10501 atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg ctcaatgccg
    10561 gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc attgcgatcc
    10621 tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc acgtttgaat
    10681 agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct gccgagcacg
    10741 tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag gtaatcgttc
    10801 atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa tataaaaatg
    10861 aaccctcaac gcttccctca atgtttgggg agccatgcgg gttcaatcat acacgggaag
    10921 cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt cgacgcgctt
    10981 catccagctg actggctttc gcacgaatgc gtgcttccag ctggtcgatc atatcgttgt
    11041 tgtccagacg gtctttgcgc acgccatctt catagaggcc gcttttcttg ttgccgccgg
    11101 tgacgccgag tgtagaaacc agcgcctcac ctgggccatt caccacgcag ccgataatcg
    11161 aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta accgtaccga
    11221 taacatcaaa ttcctgacgc gaacaggtcg ggcaggcgat gaagttaatc cctcgcgaac
    11281 ggatacgcag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc ggatcggcag
    11341 ccagcgatac gcgcagcgtg tcgccgatgc cttcagacag cagcagtcct aaaccaacgg
    11401 cggattttac tgccccgctg cgtgcaccac ccgcttcggt gatccccaga tgcagcggct
    11461 gatcgatctg ttttgccagc aaacgataag actcaacagc gaggaagacg tcagacgctt
    11521 tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca tgacgcatgg
    11581 cggattccag caatgcctgc ggcgtcggtt cgccatactt ttcttgcaga tctttttcca
    11641 gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca cagtcaacca
    11701 ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga caatcgacgc
    11761 cgtattccgc tactttcagc gcaatgcgat agtcaaagtg gatgtcagcc accagcggca
    11821 cgttaacccg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc ggtacggaga
    11881 cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc gttgcttcga
    11941 catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca tcgccaatcg
    12001 gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc tggttatgca
    12061 tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg cattgagggt
    12121 cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt tcccttgata
    12181 ctggatctgt actgcggctg gcgcaccaat tttcagtttg tacggtgcct ggccggttaa
    12241 gttcaaatta ccgtctttac gctgcatacc gctaaacaat tttttaccgg tagcatcagt
    12301 gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat cagccaccgg
    12361 cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg ttgttgctgc
    12421 cgttggtgcc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa caaccgcatt
    12481 ctgttgcgga tcaacagctg gtgctggcgc agttacggca ggtgtttgcg tgttggttgc
    12541 ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag ttgtcgacgt
    12601 atttaacgga acactctgcc cctgctcgct attactgctc agttccgccg aagattgatc
    12661 ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc aggcaccgct
    12721 caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc cgtcacgttt
    12781 tttgcggcgt ttaccgaggg aaaaactctg catcggcgca actttcgcag cccgtagtgg
    12841 agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca gacgcgcata
    12901 agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct tatcttcttc
    12961 aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct gctgactaag
    13021 tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt cattttggtc
    13081 gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg agatgccggt
    13141 gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta tcatacagta
    13201 taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact tactgttgca
    13261 tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc agacaattgc
    13321 aacttaatac ttcattcata aaagattacg ccacggtaca taaagtaacc gtggcgtaat
    13381 ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg cagggtacgt
    13441 ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcgat atcatcacca
    13501 cgagttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga gaaacggtcg
    13561 atacggctgt tcgaactgcg tccatacggc gcgcccggga acgggttcca cgggatcagg
    13621 ttgatcttac acggcgtatc tttcagcagc tccgccagtt ggtgcgcgtg ttcagtgccg
    13681 tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt ggcgttggat
    13741 ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt tttgttgatc
    13801 ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc cagtgcaacg
    13861 tcgatcatat cgcccagttt atccagcgcc ggaactacac cggaagtaga aagcgtgacg
    13921 cgacgtttag acaggccaaa accgaaatca tcaagcatga tttccatcgc cggaacgacg
    13981 ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt gattgggcgc
    14041 tgaccggtga cttttgctgc gccgacgatt ttcgccgcac gccacacctg gccgataatt
    14101 tccgacaccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa tttacactcc
    14161 agcgcacacc ccacctgcga agagacgcag agcgtggcac ggtcgtcttc cgggatatac
    14221 accgtttcga cgcgctgatc gccaacggcg atcgcccatt taatggtgcc gtcggatgaa
    14281 cgctgttctt caaccacttc cggtgcgcgg atttccgcca cctctttcag tttgccgcgc
    14341 aacactttgt tgatgtcggt catctcatca aagttgtcgc agcaatagtg atacatccac
    14401 ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa ctcccgcatc
    14461 tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac gttttcaggt
    14521 gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta tggcccctgg
    14581 agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc tgcattgtac
    14641 aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat taacgggtgc
    14701 gcgggcacac ttcgccttcg ccaaagaaat aagcgatttc gcgagcggca gattcgacgg
    14761 aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg cgcagagtac
    14821 cagccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc tgaacggcgt
    14881 tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca accagaccat
    14941 caaagaacgg ttttccatcg tgttcagcat aaaagccacg tgcctgttca acggtcaggt
    15001 gcagcatttt ggtgccaaca attttgaacc ctgcagcttc aaagcgcgca aagatattac
    15061 caatgacgtt ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt tcaatagcca
    15121 tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat ggcgcggatt
    15181 ataatgagca acagggccgt tgactattga tgaaggtaac atttttttaa aataaaatga
    15241 gttttagcaa caatcaggga caaaacagac tattgcatga caaatttcac tgtcgcgatt
    15301 tgccccacat catccatcac cagcaattga taatcacctt tatccgtcaa atgcagggtc
    15361 acgttgcgcc cgcgttcagt taacggttcg ccattcagaa accaccagcg ttcacctgcc
    15421 cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg tttaataatc
    15481 gcgccatcgc gaacgcctgt cagctgcaga gggagctggg catcgtgacc gtatggcggg
    15541 caaattgtcg aggccggtgg taagcgcaca gcgcgacgtt ctgatgcggg cagccagggt
    15601 tccagcggta gcggccagac atttatcatt tcttgtcgcg cttgcgggca atcagcggca
    15661 acacgtttgc cattttcatc cagccagatg gggaaacgaa tgccattaat gccttcctgc
    15721 tccggcagta atagagttgg cggctgactc ccgtccagca gccaggttgc caggcggcgg
    15781 cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcagat aacgccacga
    15841 gtgactgagt tcgggcgcgg gtcttccggc agattcgcac tgcgcgacag taagatatta
    15901 ttgacctgat tcaacaatgg tacggcactg gcaaagccaa actgaccaac aacgggcgtg
    15961 ccgtccggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc aatcgcccag
    16021 gcgtcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac gcgcggcaag
    16081 gcactatccg gcaagggttt cgcttcatca gccataatcc ggcgaatgat ccacgccgcc
    16141 cccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag gctgtaagcg caatttgcct
    16201 gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca tatcttccag ttttgcacca
    16261 gcgccgccga gaatgagtga aagattcggc gcagcaccgt tgggcaaata taacggcaat
    16321 ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag cacctgcaca
    16381 gcaggtaagt tcagcgagcg caccagcgcc tcgctcatgc tgatcgggcc atgaaaaccg
    16441 ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg ggacgtcttg cagcagtgat
    16501 gccgggtgga tcaagccttc atccagcgcc agaccataaa caaacggttt gagcaccgat
    16561 cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc atcgttgaga
    16621 tcaaccgatc ccacccagcc gcgaacacgc atatcggtat gatcaaccac gatcatcgcc
    16681 agtgagctgc gcggtggcaa ccgccctttc cagttttgcg ccagttcttc cagacgtcgt
    16741 tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc gagcatcatg
    16801 cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat gggttcttcc
    16861 cttgactctt ttacctgctc acgggaccac acaccttgca cggccatccg ttcgagcact
    16921 ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgaagacg gctgggcgct
    16981 tgcggcaaaa ccgccagcat tgccacctcg gaatagctta aattcgcagg cgactttccg
    17041 agataagccc aacttgccgc accgatcccc tgcaacgtac cgccaaacgg agcgcggtta
    17101 agatacaagg tcagaatttc acgcttagac agatgccatt ccagttgcaa cgcgcgccag
    17161 agctggcgaa ttttgccgcc aaatgttttg gggtgaggat caagcagacg agcaacctgc
    17221 atagtgagcg tgctgccacc ggaaataacc cgtcccgaag tgagatcttg ccatgctgcg
    17281 cgcgccaccg agaatggatt cacccccgga tgcttccaga accagcgatc ttcataattg
    17341 atcagcgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata acgccagatg
    17401 ccgtcagcat cggcgaagcg ccatagcggc gtaccatcct gcgccacgac cactcgtgcg
    17461 ggattgactt catgcagcgg tagaggccag agtttatctg ctccccacgc tgcaagaaaa
    17521 aggagaaatg gcgcagctgc cagcgttatc cagcagccgc gtttggttaa caagcgaggc
    17581 atttacggtc ggacaatcag cagatcttca gccgcgccgg tcgcccgcca ttggggaaca
    17641 tacattgatt ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac cgcccgcgcc
    17701 agatacacca gcgttaccgg ttggtattca tcaacggcaa ccgccgccac aaagcgatcg
    17761 tcacggaact caatgtgctt aatactcgcc tgctgcatct ggttcagtaa gttctgcact
    17821 tcgccaccac tttgctccag gctggcgcta ccgttcgcca gattctggtt ttccagttcc
    17881 agacccgcag gcagcagatc cacgaccaac gcatccggca cgctgttact ggcttttacc
    17941 tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt ccagcgattt gctcttacca
    18001 tcagtaccaa gaatatgacg ctcgatttgc agcacattgt tcgccggtaa aggtgcggat
    18061 tgcggataac cgctggcatc cacacgcagc cataacggct gatcgccact gtttctcacc
    18121 tgcaaggtgg caagttgatc gctattcaga ttgctgttta gcgttttctc gcctgtcagc
    18181 ggctcagctg agaaagaggt ttgcgcctgc catttacccg gtaaatcctg aatcgtacgg
    18241 gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc aaatgcctgc
    18301 tgcgaaagcg tgttcagcaa agtgttctgc tcatcgggta gcagtttatt ttcttccagc
    18361 aaggagagca ttaacgcgtt gtcgcgcagt ggactaccgt aatcacccag ccatatccgc
    18421 tcatcactat ttctcggcgt tttcagcgcc agcacaatcg cttcttcacc acgcgttgca
    18481 tcacccatgg ttttcagcgc aacgccaagt tgcagcagcg gtaaaccaga agcggcatct
    18541 gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg acgggccagc
    18601 accagcgcag cgtaagactg aacggcgaat ttactggctt tgagattatc cgcgtacggg
    18661 atcgacatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc ccggttaatg
    18721 gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc catcacgtaa
    18781 gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc gccgttatca
    18841 cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg tttctcatca
    18901 ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt tggtataaag tgacgggaac
    18961 aggccgctgg cggtttgctc aagacagccg tacggatacg cttttaactc tttgatataa
    19021 cgtgcaatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag cgtaacaggc
    19081 gagaagtttt gcaatccatc cgccggaatc gcccatgtct caccaggctg taacgccgta
    19141 ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc cgattttcca ctgcttatgc
    19201 tgatcggcaa cggtttcacc cggtaacgct aacccgctaa tggtggcctg aatttctcca
    19261 tcgccataac ccggcaatgc tcgcaccggg ataaacagcg tagtacgcac acctggcgct
    19321 aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc ggtcagggca
    19381 acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag acgcgaggta
    19441 tcgccactcg ccataaagcg cggcatgttc agttcagcaa tcaccggtgc ggcaactatc
    19501 actttgcttt cgttgctacc gaaatcatct gccgtccagg cttgcgccat gacgcgcaat
    19561 tcaccgttaa aatcgccaat cggcagtgta accgagtctt cgccctgttc gttgagcgtt
    19621 accggcagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt accaccacgt
    19681 ttcagctcat caccatcgcc accgaaacgc agagctgcca gacgcccctg accttcaata
    19741 acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa gaacgcctgc
    19801 cacggatctg gcgtgacgta gtcagtaata ttcagcacac cactatcaac ggcagacacc
    19861 agcacattca cctgtttagg cttctcgcca tttttagtgc tggctttaat tttcacggtt
    19921 aatggttgat tgggacgcat ttttgctggt gtttccagcg ccagatcgag gcgacggttt
    19981 tcatcgccaa gcggcagatg caacacacca accgcgcgtt ttggcgtcgc ggagcgagat
    20041 ttatcgccag gacgtaccac cagcgtactt aaatacagat catgacgatt ccaggtttta
    20101 tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg ccaccacagc
    20161 ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc ggcgatatgc
    20221 aacttaatgg tgtcgccagg gcgataactg gctttatcca gtttcagcgt gacacggtcg
    20281 ggtcgcactg cgccgctacc gtcgctgttg tcctgccagc tatagccagc ccagaaacga
    20341 acactactga ccgcttcatt cggcgctttg acttccagac gataagcgcc ccactctacc
    20401 ggaaaactga ccttgccggt ttcgtccgct ttcagatcca gagtttgttc attttcgatc
    20461 agatcttttt gatcaaactg tgactgccag ccttcatctt ctgaccagtt ccagtagtaa
    20521 tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt cacgccttgc
    20581 gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat gggctgtttc
    20641 accgtgctgt cagtacgata atcgtaaacc gatttcgagg cgaactgcgg acggatcccc
    20701 ggcaatgcat cggcaggcca gatagcctgc tcagcgcggc gcgtcaccgg gcgaccgccc
    20761 gattccagca aactaccctg gaaaataacc tgtaatgggg aatgcgtttc cttccactgg
    20821 ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac ttcatccagc
    20881 gtgcgggaaa gattttcggc agctatatcg ccgaattcaa aaccaggtaa ggctgacaca
    20941 gcttcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt agcaggtgca
    21001 ccatacaggt agtaccccac cacggagaat ttcacttcat ctttcggcgt tagcggggtt
    21061 ttctcaccgg tcagattcag cgccatgcgc tctggcataa aatcttcgac gtggaaatcc
    21121 cacatccgat actggttatc gcccgtgtta gcgcgaatat gccacatacc ggttgccgca
    21181 ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact aacgacgctc
    21241 ctgagtacct gcccatcggg tttaatcacg tctaacttga tgggttgatt gggcaacgct
    21301 ttaccgtctg catcacgcag caaaccattg aggattaccg tttcacctgg gcgataaaga
    21361 tcgcgtgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc aatgttaaat
    21421 tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc gtctttacgc
    21481 gccaacagta atgccgcgtt tttatcattt tccagctgca catgcccctg agcgtcactg
    21541 gttgcctgag tcagagtctg ccctttctca tttaataaag agacttcaat tccttgctgg
    21601 gccgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata acggtgagct
    21661 gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc gtaacgtcca
    21721 gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat atcgcccagc
    21781 ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg tccggtgtag
    21841 accagatccg ccatctgcag cagtttgtct gactgccagt tcgccagcga attgcggtat
    21901 tcccattggc taatgaatgc tggcagagat tctggcttaa cgcggaagaa gttaacatcg
    21961 acattattaa cgttgagcgc cattaccggc agcccttcaa cgactttgcc tggcagcagc
    22021 gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc gggtagttat agttttttcg
    22081 taatctttac tgaaggttgc gttgttgagt gctttgacct ccttgccaat agtaacgatc
    22141 aaatcacgtt tcggttcgag gtggcgtaaa cgtaactctt taagattatc tgacagctcc
    22201 caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg tgagaaatcc
    22261 tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcacc atcaagttgg
    22321 acttcagaga gatccagcaa tgtcagcacc ttaccggcac tctgttgggc cagtttttgt
    22381 ctctccggca cggagagttt tgctgaactc gcgttttctg aagaggccgc tttagtaact
    22441 tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc gcaccctgcc
    22501 agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca tccctgccac
    22561 aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt atgtaattca
    22621 gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag ccaaatcagc
    22681 ctctttcgct tgtcgctttt cactaaacat ccgacaattt agcctaaccc cggcaaaaat
    22741 ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg aacatattga
    22801 tgacccggaa attcagatta ttgatgcccg catggcgtcg cctggacagg aagatcgtaa
    22861 cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg atatcgaagc
    22921 gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa cgttcgccgt
    22981 ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg acgaaggtaa
    23041 tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag agaaagtgtc
    23101 gattctgggg ggtggacttg caggctggca gcgcgatgat ctgctgttag aagaaggtgc
    23161 agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagccg tggtgaaagt
    23221 aaccgatgta ttattggcaa gccatgaaaa tacggcgcaa attattgatg cccgcccggc
    23281 tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg gacatattcc
    23341 cggagcactg aatgttccgt ggacggaact ggtgcgcgaa ggcgaactaa aaacgaccga
    23401 tgaactggat gcgatatttt ttggtcgcgg cgtcagctac gacaaaccaa ttatcgtcag
    23461 ctgcggctct ggtgtaacgg cagccgtggt tttgttagca ctcgcgacgc tggatgtgcc
    23521 aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcgcgggcag atttaccggt
    23581 tgagccagtg aaataagtat tttacaggca ataaaaaacc gccgaatttg gcggtttttt
    23641 attgctggtc tggttcgcgg cctttccagc aggctgtatt accgtagtaa tgcaagcgcg
    23701 tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag agagttatta
    23761 ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag cgggcgctgg
    23821 cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct tgctatcgcg
    23881 gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac aatgaagata
    23941 cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt aaataataac
    24001 cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag caagtaagcc
    24061 cgaatgccgc cctttggggc ggcatatttt agattatccg attctgttta aagtcacgca
    24121 aaaaaccacc ccagcgacgt tcatagaatg gcacaatatg ttcggtaata aagtggctaa
    24181 ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca ggtaatgtat
    24241 cggtcgctac acttcccatc gcctgaataa tttcttcgat atcaccatcg gcttcaatgc
    24301 caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata aaagcacgct
    24361 tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt gctggcggct
    24421 ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg ctgctcagcg
    24481 gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct ttaccggtcg
    24541 gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat gtgcgaacgg
    24601 gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct aacgaggtga
    24661 aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca agcgcactat
    24721 cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg gattccagta
    24781 gagtacggaa aaaggccggg cggtgcgccg gttcagttgc tgctttttcc agcaggtctt
    24841 caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc ggggcgcaaa
    24901 tgcctgatgc gctacgctta tcaggcctac aagggattcg caattttttg aatttgcaga
    24961 atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac gccgttaaca
    25021 gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt tcaaccggcg
    25081 ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag ttctcaacaa
    25141 agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg cttccggtat
    25201 tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaactccgcc agcggcagac
    25261 gccagaacgg ttcgttctcc tgcgcggcac tcgccagcaa gcgaccggcc agcgcatcgt
    25321 caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc ccggtgaggg
    25381 tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga ccatcggcaa
    25441 gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg ttgcgatagg
    25501 tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca cagcagagga
    25561 acagcttcac gcgcttgttc agtccgcgcg taatggcaaa tgccagcgcc ccggtaaccg
    25621 ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt ttgatgctgt
    25681 agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc ggtgcttctt
    25741 tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct gaaccgcgcc
    25801 cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg gtgatccgat
    25861 aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt gccagttgcg
    25921 atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg atgatcatca
    25981 ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc actttacgcg
    26041 tgcctttcgg ggctttgtaa ccttgccaga atgcccagca gcgatccgca tcccagcctt
    26101 caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgcgcc gcacgctgga
    26161 tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta ttaatgctgt
    26221 aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta atcttcatcg
    26281 cttctgtcat tttagttatc cttctttgta aaagggccgc cagttggcag ccccaggatt
    26341 tttccgctaa atgattcgcg ttgcaggaag gcggcaagtg agtgaagccc caggagcata
    26401 gataactatg tgactggggt gaacgagcgc agccaacgca tctgcggcgt gaagcatgac
    26461 gcggaaatta ttcggcctcg tccagccaga ctaacaaaat cgcttcgagg attttctcgt
    26521 tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga tgcatatcgg
    26581 tgaatcgaac cgttttcgga tcaagatcag gatacgcatc gtacagtgct tcgccaattt
    26641 cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac gcgcatggtt
    26701 gatagtgtaa cgcgggattt cgaccactaa atcttcgtcg gtgacgcgcg cctggcagct
    26761 taaacggctt tccggctcca gtccccaggc tttgtccagc atgtcgtctt cctgctctga
    26821 gctttccggc agtgagtcaa aaccttcacg aacgatgcag tggcaggtgg tacaagcaca
    26881 ggatttttca caggcgtgtt caatctcgat accgttacgc agagctgcgt cgagaatggt
    26941 ttcaccgcta ttagcttcca gaacagcgcc atcagggcag agatcctgat gaggcaaaat
    27001 aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca cgacgaaccg
    27061 actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt ttaatcgctt
    27121 gttcgatggc gtcaacatca tcgccctgcg ccacttcact caggtgagcg gcagcatcgt
    27181 caatgacctg acgttctgcg tcgcttaaca gcgcggcatc agcagccagc gcgccgtgca
    27241 gactttccag cacacgcgcc gcttctactt tttgttctgc cagcattcgg gcttttacgt
    27301 cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta tcggtcagac
    27361 cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc atcgccgtca
    27421 cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca ccaccagccg
    27481 gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc tgcaccagtt
    27541 cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta aaggtggtga
    27601 aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc tccaccaggc
    27661 cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcagcagc atttcgctgt
    27721 ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg acgactttat
    27781 ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt tcacgcacca
    27841 gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcgtca gcttctacac
    27901 ccgcgtcttt aagcgcgcga cgacaagcca gtaaggttcg ttttaccagt ggcgcgatca
    27961 gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta acggtcacgg
    28021 agtccgcatc gctcagcgcg attttggctg caatggcggc atccagcagt tcacgctgaa
    28081 cgcggttatc gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc gccagcagat
    28141 ggtcgaaatc atcaccaccg agcgcggaat caccgccggt cgccagcact tcaaacacac
    28201 cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc gccgaggtca taaacggcga
    28261 tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcggtc ggttcgttaa
    28321 gtaagcgcag gacgtgaagg cccgccagac gcgccgcgtc tttggtgccc tgacgctggg
    28381 catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct gccagggctt
    28441 cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc gggttcagca
    28501 gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat tgataaggca
    28561 gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcaggcgt ttaacagaac
    28621 taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc accgaatgcc
    28681 cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg tcggctaacg
    28741 tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc aggtcaatac
    28801 cagccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta atttgtaata
    28861 aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag ttgttcggca
    28921 ctgctccgca gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc cgcgtcccac
    28981 gcctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat ctttttcaca
    29041 cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc gtccagctct
    29101 tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg ctgctcgctg
    29161 gcgagatcaa aaccgtgtaa agaaagcaaa tattccgcgc gcattaacgg atgacgcagc
    29221 gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg ttccgcctgg
    29281 cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa acgcaggctc
    29341 agcgcctggg tatcgagttg ataacgggca ggcaagccaa agagggtgaa gtaatccata
    29401 acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca aaccccacgc
    29461 gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt tcgccgcaac
    29521 cacactcatc tttgacattc gggttggtga atttaaaccc ttcgttcagg ccttctttta
    29581 cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc acgactttca
    29641 cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat tccagtacat
    29701 aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac cctttgccgc
    29761 ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta atcgacataa
    29821 ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat ggcggctttg
    29881 atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc aagttcttca
    29941 gcaatatcgg tatttttgat cgcctgcgct tcgtcgagag acttcccttt cacccattcg
    30001 gtgaccaggg agctggaagc gatagcagaa ccgcagccgt aagttttaaa acgcgcgtct
    30061 tcaatgatac cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc acaggccggt
    30121 gcccccacca tgccgctgcc gacgttctcg tcgttgttgt caaaggaacc cacgttacgc
    30181 ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc tcctgattcc
    30241 gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg cttgtacatt
    30301 tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa ctcgatggtg
    30361 tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga gctatgtgcc
    30421 agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct tgctgacgta
    30481 caggcggaac ctgaagaaac tgcgaggtct ttcagcgcca taatcagcga ctcaccttca
    30541 acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc accgttcagg
    30601 taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcaggcc gcgcagacgt
    30661 tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cctcgcccat tccgacgatc
    30721 tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc gtgcatttgc
    30781 gcttcgatgc gtacgcgcgg tttacgacgt acatacagcg caccgatacc tttcgggcca
    30841 tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag gtcgataggc
    30901 agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc acggcacatt
    30961 tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg catgatggaa
    31021 acaaggatgg tgtcgtcacg catcgctgct tcaagttctt tcaggtcgat aatgccgtta
    31081 cgctgcggtg ccaggtaggt gacttcaaaa ccttcgcgct ccagctgacg gcaggtatcc
    31141 agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt ctgataaaag
    31201 ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt aaagacgatt
    31261 tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc taccgcttct
    31321 tcagcctgcc agccgaaacg gtgagaacga gaggccgggt taccaaaggt tccgtccatc
    31381 gtcatgaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt tgcggagtag
    31441 tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca atgcaaggaa
    31501 tcaggctacc ggctggatgt acgaccgtgt ttacggagcg cgatacgccc cggcctgatt
    31561 ctaatatctt gttatcttta acctaactgt ttcgggtttc agcaagttga caaacttacg
    31621 aatctccggg agcttacata agtaagtgac cggagtgagt atgtgcagtc aacgcagatg
    31681 caacacgaaa caggacggtt aaattaagcg cgtaacttaa cgtcgatcgc gtcttgtgtg
    31741 cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca catccaacac ttcctggtta
    31801 ttaaccagtt cgcctaaagt aatgttgttg agaaaaccgg tgagacggtc gctcaaatca
    31861 cgccacagcg cgtgggtcag gcatttatcg ccgccctggc agccgccttt accctgacaa
    31921 cgggtagcat ctacagattc gtcaacggcg ctaatcactt cgccaacggc gatgctgctg
    31981 gcatctttgc ctaacagata accaccgcct ggtccacgta cgctggaaac cagaccattt
    32041 ttacgcagac gggaaaacag ttgttccaga taagaaaggg aaattccctg acgttcggaa
    32101 atatcagcca acggtaccgg gcccgcttca gagttgagcg caacgtcaag cattgcggtc
    32161 acggcatagc gccctttaga tgtcagtctc atgtcttact tcacctcaaa ctcgcccctg
    32221 cccggggttt tttattgtaa aagtgggggt attgcatagc agggtcaagt ctgacattcc
    32281 cgagtaaatt ggtcaactat ttacttgact gatttagtcg ggtatttaac cttcagtgcc
    32341 atttttttat cgtggcgttt gtacctgttg tcggatgcgg tgtgaacacc ttatccgacc
    32401 tacggttctg tacattgtag gcctgataag acgcgttaag cgtcgcatca ggcaactact
    32461 gttattcggc cttgttacct ttattctgct gctcaataga agccagaatc ccgcgcagga
    32521 tattcaattc ctggctttcc gggcgcgcac gggtaaacag acggcgcaat ttattcatca
    32581 cctgccccgg atggttttca cggataaaac cggttgccag cagcgtttgt tccagatgac
    32641 cataaaaacg ctccagatca tcgaccagcg gatacggcgt ctcttcatgc tcgacctgct
    32701 cgccgttttc ctgagttgcc agccaggcca tgcgcacttc ataggcaata acctgaaccg
    32761 ccatcgccag gttcagcgag ctgtattccg ggttagccgc aatcgcgaca tgataatggc
    32821 atttctgcaa ctcttcattg gtcaagccga cgcgctcacg accaaacacc agcgccaccg
    32881 gggtatttgc cgcttcagcg acgcttttca gaccgcattc gcgcgggtcg agcatcggcc
    32941 acggcagcgt gcgggagcgt gcgctggtgc ccaccaccag gctacaaccg gctaacgctt
    33001 cgtcgagcgt atcgacgatg tgagcattac caatcacatc gctggcccct gctgccaggg
    33061 caatcgcctg ggagtcgggt ttcaccagtg gattaaccag ccacagattg gttaatccca
    33121 ttgttttcat ggcacgggca acagaaccca tattgccggt gtgtgacgtc tccaccagca
    33181 caattcgaat attttgcagc attgtctttc ttcatctaaa gattattcac gcatcttatc
    33241 ataaaacgaa gacagatgcc gatctcgctg ctatactctg cgccgttttc ccgttcttta
    33301 atatccagtg agagagaccg atgcatccga tgctgaacat cgccgtgcgc gcagcgcgca
    33361 aggcgggtaa tttaattgcc aaaaactatg aaaccccgga cgctgtagaa gcgagccaga
    33421 aaggcagtaa cgatttcgtg accaacgtag ataaagctgc cgaagcggtg attatcgaca
    33481 cgattcgtaa atcttaccca cagcacacca tcatcaccga agaaagcggt gaacttgaag
    33541 gtactgatca ggatgttcaa tgggttatcg atccactgga tggtactacc aactttatca
    33601 aacgtctgcc gcacttcgcg gtatctatcg ccgttcgtat caaaggccgc accgaagttg
    33661 ctgtggtata cgatcctatg cgtaacgaac tgttcaccgc cactcgcggt cagggcgcac
    33721 agctgaacgg ctaccgtctg cgcggtagca ccgctcgcga tctcgacggt accattctgg
    33781 cgaccggctt cccgttcaaa gcaaaacagt acgccactac ctacatcaac atcgtcggca
    33841 aactgttcaa cgaatgtgca gacttccgtc gtaccggttc tgcggcgctg gatctggctt
    33901 acgtcgctgc gggtcgtgtt gacggtttct ttgaaatcgg tctgcgtccg tgggatttcg
    33961 cggcaggcga gctgctggtt cgtgaagcgg gcggcatcgt cagcgacttc accggtggtc
    34021 ataactatat gctgaccggt aacatcgttg ctggtaaccc acgtgttgtg aaagccatgc
    34081 tggcgaacat gcgtgacgag ttaagcgacg ctctgaagcg ttaatgactc aggcgggtga
    34141 tgtcactcac ccgccctcgc ctttcaggcg ctattctgaa atacttcctc accgctttac
    34201 tttctttcgt cactctccca ccatctttcc ccgattaatg gatgataaag aagtcgcaag
    34261 gaaaggttat ggcactgcca gtgaacaaac gcgttcccaa aattctgttt attctctttg
    34321 ttgttgcctt ctgcgtttat ttagtgccgc gcgttgccat caacttcttc tattatcctg
    34381 acgataaaat ttacggtccc gacccctggt cggcggaatc cgtcgaattt acggctaagg
    34441 acggtactcg tctgcaaggc tggtttattc cttcttcgac gggccctgct gacaacgcca
    34501 tcgcaaccat cattcatgct cacggcaatg ccggaaatat gtccgcccac tggccgctgg
    34561 tcagttggtt acctgagcgt aatttcaacg tttttatgtt tgattatcgc gggtttggta
    34621 aatcaaaagg cacgccgtcc caggccggat tgctggacga tacgcaaagt gccatcaatg
    34681 tggtgcgcca tcgcagtgat gtaaacccac aacgtctggt gctgttcggg cagagtattg
    34741 gcggggcgaa tattctggat gttattggtc ggggtgatcg tgaaggcata cgtgcggtga
    34801 tcctcgactc cacatttgcc tcttatgcaa ccatcgccaa ccagatgatc cccggcagtg
    34861 gctacttact tgatgagagt tacagcggcg aaaattatat cgccagcgtc agcccgatcc
    34921 cgcttttact cattcacggt aaagctgatc acgttatccc atggcagcac agcgaaaagt
    34981 tgtatagcct ggcaaaagag ccaaaacggt tgatcctaat cccggatggc gaacacattg
    35041 atgctttttc cgatcgtcac ggcgatgttt atcgcgaaca gatggtggac tttatcctta
    35101 gcgcgttgaa tccacagaac taacccatga tcgctagcac gataatcatt cacaaaacca
    35161 ccttaagaca tgctaatcca ctggtcagaa cagtttaaga tgagaaaaat tctgtgacgc
    35221 ttgccaacat ttctgatgat tagcattccc ttcgccattt ccctgagcaa actttagcta
    35281 ttcttatcaa ttatgcttat gggagatcta cagatgatgc ctacgcttgc tccaccatct
    35341 gtcctttcgg ctccccagcg ccgctgccag atcttgctga cgctctttca gccggggttg
    35401 accgccacca cggcaacttt cagcgagctt aatggtgtgg atgatgatat tgccagtctt
    35461 gatatcagcg aaacaggaca ggagatcctg cgctatcatc aactcacact gacggctggt
    35521 tatgacggta gctaccgggt tgaaggtaca gtgcttaacc aacgtttgtg tttatttcac
    35581 tggctacgac gtggtttccg tctgtgtcca tcatttatta ccagccattt cacccccgcc
    35641 ctgaagagtg aactgaagcg gcgcggaatt gcgcgtaact tttacgacga taccaatcta
    35701 caagcgttag tgaatctctg ctcccgacgg ctgcaaaaac gctttgaaac gcgcgatatt
    35761 catttcctgt gtctgtatct gcaatattgt ttgctgcaac accacgctgg aattacgcct
    35821 cagtttaatc cgctccaacg tcgctgggcg gaatcctgcc ttgaatttca ggtagcgcag
    35881 gaaattggac gccactggca gcgtcgggcg ctccagcctg taccacctga tgagccactg
    35941 tttatggcac tacttttttc catgttgcgg gttcccgatc cactgcggga tgcgcatcag
    36001 cgagacagac aattgcgtca gtctatcaaa cgtctggtaa accattttcg tgagctggga
    36061 aatgttcgtt tttatgatga acaggggtta tgtgatcagc tttataccca cctcgcccag
    36121 gcgttaaatc gcagtttgtt tgccatcggt attgataata ccctgccgga agagttcgcc
    36181 agactgtacc cacgcctggt gcgcaccacc cgcgcggcgc tggccggatt tgaaagtgaa
    36241 tacggcgtcc atctttctga tgaggaaagt ggtctggtcg cggtgatttt cggtgcctgg
    36301 ctaatgcagg aaaacgatct gcatgaaaaa cagattattc ttcttaccgg gaatgatagc
    36361 gagcgagaag cgcagattga gcagcagtta cgcgaactaa cgttactgcc gctcaacatt
    36421 aagcatatgt cggtaaaggc atttttgcag acaggcgctc cgcgtggcgc ggcactgatt
    36481 attgcgcctt ataccatgcc attaccgctc ttttcaccac cgctgatcta tacggacctg
    36541 acgctgacaa cacatcaaca ggagcagatc cgcaaaatgc tcgaatcagc atgagggaac
    36601 aactttcggg cgcaaaaaca ttgccggaag cgccaccagc gccattaccc agaacacgcc
    36661 gtggcccaga tattgataca ggaaaccggc gaaaacggtc atgatagcga tactgccgcc
    36721 catcgcgacg gcagagtaaa ccgcctgtaa acggatgact tcgctaccct ggcgagcagc
    36781 aatataacgc atggcggcca ggtggcagac cgtgaaggtg ccgcaatgca gaatttgcac
    36841 cactatcaac cacggcaacg ccgtagttgc tcccataatg ccccagcgca ctacgccgca
    36901 aatcgccgag atcaacagca tatcgcgtgc actacaacgg cggaaaagtt tattactcag
    36961 cgcaaagata atgacttccg ccaccacgcc cagcgaccac aaatacccca ccgccgaggc
    37021 cgagtagcca gctgcctgcc agtaaatggc gctaaaaccg taataggccg catgtgcccc
    37081 ctgcaataaa caaacgcagg ccagaaagcg ccagttctgg agaaccagcg ccaaccacgc
    37141 tgaccaaccg gtgctctcct gctggcggct tgccccttgt ggctgaatcg tcggacggat
    37201 gagaaagccg agcagcatgg atgccacgcc caatgttaac agcgcgagga tcacctgata
    37261 atcaaacata gtgaccagtt tgcccgtcag cgccgagcca atgacaaacg ccaccgagcc
    37321 ccacagtcgc actttgccgt aatcaagcgg gaactgcttt tgccacgtat tcgccagtgc
    37381 atcggtcaac ggtaccagcg gtgagaaaaa gaggttaaag ccaatcatca ccagcatcag
    37441 ccacgctacg tgcgcccccg cccagaaggc gacagcaaag agaagtgtca tcattgccag
    37501 cacgcgcaag gcggaaatca ggcgagaagg atcgctgacg cggggcgcga tgagcaaact
    37561 cccgagaaaa cgggcaacca gacctgcccc caataacagg ccgatggttt ctggcgttaa
    37621 accaatccct ttaagccaga cgctccagaa aggtagaaaa atgccgtaac taaaaaagta
    37681 tgtgaaatag ccgagcgcca accagcgcgt ggattgcaaa accatgagtc cctcccgtca
    37741 ggaggcgtta gtctggggtt aatcgaatgt tggtgcaagt tgaaaaataa gaatattcga
    37801 gcctgcttcc cgatgtctgt aacgccagtt cacaagcact aaaaaactgc gatccgcgac
    37861 gcatgttgca aaaataatgc gtaattatgc cgttacgttt gccaaacgtt cctgcacaat
    37921 ggcgatgaaa tcgcgtaatg ccggcttcat ctcccctttc ttccacgcca tcagtaaagc
    37981 aatggaaggc acatttccgg caatgggacg aaaaacaacc tgtccggtat taaaattatt
    38041 catataaccg ggtatcaaag tgacgcccag ccccatgccc accagattca tggtcaccag
    38101 aatattcgtt gccacctgga cgatatttgg ctggctgttt tcttgcgcaa accacgcttt
    38161 aacgatcggc gcaagcgaac cggaatacgc cggatcggta ctgacgaaat tcacgccatc
    38221 cagttgcgcg gcggtgatct ctttttcatg tgctaaaggg tgatcaaccg gtaacacaac
    38281 cactaatggt tcgtcaaaaa gctccagata atcaatctcc gggctataaa caggatggcg
    38341 cattaagccg acgtcgagtt caccacggcg aattttttcc tcctgttgcg tggtgattaa
    38401 actcaccagc tcaatcaagg tgtctggctg tctgagacga aacatcggta atacttttgg
    38461 cagtaaattc acttccgccg atggcacaaa gccaatggtt aattgtctgt cttcctgaac
    38521 aattttccgc gcccgtaatt tggcattttc cgcttgttcg aggattgcca gcgcgtcctg
    38581 gagaaaacat tctcccgccg ccgtcaacgc gactttgcgc ttatccctca ccagtaacgg
    38641 aacaccgaca cagttttcaa gatcgcggat ctggctgctt aacgaaggct gtgaggtatg
    38701 cagtttttcc gccgcacggg taaagttcag tgcctgcgcc actgcgacga aatagcgtaa
    38761 atgccgtagt tccatcacct tccccttgtt atcgaaaaaa cgtctcagct ggtagaaatt
    38821 aactatttca catattagca accaaccagc aacatcctta tggcacaaaa atagaaggtc
    38881 aatacatctt atctttcagg attaaaaaat atgaccacac cctcagattt gaacatttac
    38941 caactgattg acacccaaaa tggtcgggtc actccgcgta tttataccga cccggatatt
    39001 taccaactgg agcttgagcg tattttcggt cgttgctggc tatttctcgc ccacgaaagc
    39061 cagatcccaa aacccggtga tttctttaac acctacatgg gagaagatgc ggttgtcgta
    39121 gtgcgtcaga aagacggcag catcaaggcg tttctcaacc aatgccgcca ccgggccatg
    39181 cgtgtgagtt atgcagattg cggcaacact cgcgccttta cctgcccgta tcacggctgg
    39241 tcttatggca ttaacggcga gttgatcgat gtaccgctgg aacctcgcgc ctacccacaa
    39301 gggttgtgta aatcccactg gggactaaac gaagttcctt gtgtggagag ttataaaggg
    39361 ctgatttttg gcaactggga taccagcgca ccgggcctgc gtgattacct gggtgacatt
    39421 gcctggtatc tggatggaat gctggatcgt cgcgaaggcg gcaccgaaat tgtcggcggc
    39481 gtacaaaagt gggtgatcaa ctgtaactgg aaattcccgg cagagcagtt cgccagtgac
    39541 cagtatcatg ctctgttcag ccatgcttct gccgttcagg tattaggggc gaaagatgat
    39601 ggcagcgata agcgcctcgg tgatggacaa accgcccgcc cggtgtggga aacagccaaa
    39661 gatgcgctgc aatttggtca ggacggtcac ggtagcggtt tcttcttcac tgaaaaaccg
    39721 gatgctaatg tctgggtcga tggcgcagtt tcaagctatt accgcgaaac ctatgccgaa
    39781 gcagaacaac gtttaggtga agttcgcgcc ctgcgcctgg cgggtcataa caatatattc
    39841 cccacgcttt catggctaaa cggcactgcc acactccgcg tctggcatcc gcgcggccct
    39901 gatcaagttg aagtgtgggc gttctgtatt actgacaaag ccgcctccga tgaagttaaa
    39961 gccgcttttg aaaacagcgc cactcgtgct tttggtcctg ctggttttct cgagcaggat
    40021 gactcggaga actggtgtga aatccagaaa ttgcttaaag gccaccgcgc ccgcaacagc
    40081 aaactgtgtc tggaaatggg gcttggtcag gaaaagcgcc gcgacgacgg cattcctggc
    40141 attactaact atatcttttc agaaactgcc gctcgtggaa tgtaccaacg ctgggccgat
    40201 cttctgagta gcgaaagctg gcaggaagtg ctcgataaaa ccgccgctta ccagcaggag
    40261 gtgatgaaat gagtgcgcaa gtttcactag agttacatca ccgcattagc cagtttctct
    40321 ttcacgaagc cagcttactg gacgactgga aatttcgtga ctggctggcg cagctcgacg
    40381 aagagattcg ttacaccatg cgcaccacag ttaacgcgca aacacgcgac cgccgcaaag
    40441 gcgtccagcc accgacaacc tggattttta atgacaccaa agaccagctg gagcggcgaa
    40501 tcgcccgtct ggaaacgggc atggcctggg cagaagagcc gccgtcacgc acccgtcact
    40561 taatcagcaa ctgccagata agcgaaaccg acatcccaaa cgtatttgct gtgcgggtaa
    40621 attatctgct ttatcgggca caaaaagagc gcgatgaaac attctatgtt ggaacgcgtt
    40681 tcgacaaagt tcgccgtctg gaagatgaca actggcgctt gctggaacgg gatatcgtcc
    40741 tggatcaagc ggtaatcact tcccataacc tgagtgtact gttctgatga atcgaattta
    40801 tgcgtgtccc gttgcggatg tgccggaggg tgaggctctc cggatcgata cctcgcccgt
    40861 catcgccctg ttcaacgttg gcggcgagtt ttatgccatt aacgatcgtt gcagccatgg
    40921 taatgcgtca atgtcagaag ggtatctgga agatgacgcc acggtggagt gcccgctaca
    40981 cgccgccagt ttttgcctga aaacggggaa agcgttatgc ctgcccgcca ccgatccgct
    41041 caccacttat ccagtacacg ttgaaggtgg tgacattttc atcgacttac cggaggcgca
    41101 gccatgagcg atctgcataa cgagtccatt tttattaccg gcggcggatc gggattaggg
    41161 ctggcgctgg tcgagcgatt tatcgaagaa ggcgcgcagg ttgccacgct ggaactgtcg
    41221 gcggcaaaag tcgccagtct gcgtcagcga tttggcgaac atattctggc ggtggaaggt
    41281 aacgtgacct gttatgccga ttatcaacgc gcggtcgatc agatcctgac tcgttccggc
    41341 aagctggatt gttttatcgg caatgcaggc atctgggatc acaatgcctc actggttaat
    41401 actcccgcag agacgctcga aaccggcttc cacgagctgt ttaacgtcaa tgttctcggt
    41461 tacctgctgg gcgcaaaagc ctgcgctccg gcgttaatcg ccagtgaagg cagcatgatt
    41521 ttcacactgt caaatgccgc ctggtatcct ggcggcggtg gcccgctgta caccgccagt
    41581 aaacatgccg caaccggact tattcgccaa ctggcttatg aactggcacc gaaagtgcgg
    41641 gtgaatggcg tcggcccgtg tggtatggcc agcgacctgc gcggcccaca ggcgctcggg
    41701 caaagtgaaa cctcgataat gcagtctctg acgccggaga aaattgccgc cattttaccg
    41761 ctgcaatttt tcccgcaacc ggcggatttt acggggccgt atgtgatgtt ggcatcgcgg
    41821 cgcaataatc gcgcattaag cggtgtgatg atcaacgctg atgcgggttt agcgattcgc
    41881 ggcattcgcc acgtagcggc tgggctggat ctttaaggaa gcacgatgaa agaaaaaacg
    41941 atcattattg tcggtggcgg gcaagcggcg gcaatggctg cggcctcgct acgccagcaa
    42001 gggttcaccg gtgagctgca tctgttttcc gatgagcgac atcttcctta tgaacgacct
    42061 ccactctcga aatccatgtt gctggaagat tccccgcagt tacagcaggt gttacccgct
    42121 aactggtggc aggaaaacaa tgttcatctg cattccggtg taaccatcaa aacgctgggt
    42181 cgcgacacac gagagttagt gttaaccaac ggcgaaagct ggcactggga tcagcttttt
    42241 atagcaaccg gcgcggcagc tcgaccgctg ccgttgcttg atgcactggg agaacgctgc
    42301 tttaccctac gccatgccgg tgatgccgcc agactgcgag aagttctgca gcccgaacgg
    42361 tcagtcgtga ttgtcggtgc cggaactatt ggtctggaac tggctgccag cgccacgcag
    42421 cgcagatgta aggtgacagt gattgaactg gcggcaaccg tcatgggccg taatgcacca
    42481 ccgcccgtgc aacgctatct tttacagcgc caccagcagg ctggtgtgcg cattctgctc
    42541 aataatgcca ttgaacatgt ggtcgatggt gaaaaagtag aactgacgct acaaagtggg
    42601 gaaacgcttc aggctgatgt ggtgatttac ggtattggta tcagcgccaa cgagcaactg
    42661 gctcgcgagg ccaaccttga tactgccaat ggcattgtca ttgatgaggc ttgccgcacc
    42721 tgcgatcccg cgatctttgc cggtggcgat gtggcaatca ctcgtcttga taatggtgca
    42781 ctacaccgct gcgaaagctg ggaaaacgcc aataaccagg cgcaaattgc cgctgccgca
    42841 atgttagggc taccgctacc gctactgccg ccgccgtggt tctggagcga tcagtacagt
    42901 gataacttac agtttattgg cgatatgcgt ggcgatgact ggctttgtcg tggcaacccg
    42961 gaaactcaga aggcgatttg gtttaatctg caaaacggcg tgcttatcgg tgcggtcacg
    43021 ctgaatcagg ggcgtgagat tcgcccaatt cgcaaatgga tccagagcgg caaaacgttt
    43081 gatgcgaaac tgctgataga tgagaacatc gcgcttaaat cactgtaacc aggataatta
    43141 gcgaatatct caatgcctgg ggcgtggcga ggtgcaagag tgtgtattac gtttaaatca
    43201 cattatcttg caaagggatt ggttatgaac acactacgtt attttgattt tggagctgcc
    43261 cgccccgttt tgttattaat tgcccgtatc gccgtggtct taattttcat tatttttggt
    43321 tttcccaaaa tgatgggctt tgacggtacg gtccaatata tggcctcgtt gggcgcgcca
    43381 atgccgatgc tggcagcgat tattgcggta gttatggaag tgcccgccgc gatattaatc
    43441 gtgcttggct ttttcacccg tccgctggcg gtgctgttta ttttctacac gctgggtacg
    43501 gcggtgattg gtcaccatta ctgggatatg accggcgatg cggttgggcc aaatatgatt
    43561 aatttctgga agaatgtcag tatcgctggc gcattcctgc tgctggcaat taccgggccg
    43621 ggggcaattt ctctcgatcg gcgttaggaa agatgccgga tgcggcgtga acgccttatc
    43681 cggcatttaa taaattacag ccactctacc cgcaacgaca tctctgaagt tgttgattcc
    43741 cctggtgcta gcgcaatgag atcaccccct tccgggcgat gatgatcatc cggcgcatgg
    43801 ctcatcggtt ccagacagaa gaaatcaaac gcatatcctt tatcaaacgc agggtcggaa
    43861 acaaagatga aataacacgg tgcaggtggc gtcgtttcca tgatgatggc ataaccttcc
    43921 tgcggctgct cgatgcgggc ctgaccattc catccggcaa aaccattgtt cacccactgg
    43981 cgcggcaacg gcgcgggctg gttaaaatcc agttcctgcg gtagctgctc gcaaaactca
    44041 cccgccagcc actgctccag ctccagccag taaccgctcg cctgtgcctg aatccgcgtt
    44101 tgcggcgaca acggaaaata aggatgccag ccggtaccaa atggcagcgt ctctgcccct
    44161 tgattggtga cagagagcgt caccgtcagc gtatccgccg ttaaatgaaa cgcctgactt
    44221 acccgatagt gatagacacc gctgcgatgt tcatacacca gacacaaact atcatcgctg
    44281 tgcgagacac attgccactc gcccagccag ccatcgccgt gtagatagtg cgcatcccac
    44341 tcaacattcg gttgcagttg atactcacgc ccctgccaga caaaacgatt gccgctcacc
    44401 cggtttgcaa atggcaccag cggaaaacat gaggcatcgg ttgccacacc gcttttttta
    44461 ccaggacgta agagcggcgt cgtatcgcgc cagaagcctt cgattacgcc gccctggtca
    44521 gaaacgtcca gctttagcga cccgtgggat aaggtataga tggtcatccg ctaactcctt
    44581 aatccgggaa gttaatcaca actttcccgc atttgccgct cgccatcagc gcataggcat
    44641 cacctgcctg ttccagcgag aagcgatggg taatggcgtt acgcggccac agcttccagt
    44701 ccgtcagatc atgggcgcat ttttccatat ggaacagact ggtcacccag gagccgataa
    44761 tccgccgttg atggtgcatc agatcggcgc tgacctcgaa ttccactttt ccggtttcac
    44821 caatgtaaac cacccgtccc cagtcagcgg tggattgcag tgccagcaag cgacctgcgg
    44881 cattaccgga acaatcgagc gcaacatccg cgccaccgtg ggtgagttcg gcgataatct
    44941 gcggcagacc ttcggtggtt gctaaatagc cgtgatccat cacccctaac tgttttgcca
    45001 tcgccagacg ttccggcagc atatcaacgc cgatgatccg ttttgcaccg cgacctttcg
    45061 ccagcatcat cgccatcatg ccgaccggcc ccagaccgac ctccagcact ttatcgctgc
    45121 cggaaacttc gccgcgcaaa atcccttcat aggccgtgcc aacgccgcaa ctgataaacg
    45181 caccatcttc gtagctcagc gcatccggca ggaggatcag atctttttct tccgccagca
    45241 gatattcggc atgaccgccg tcacgctgcc agccgtaagc cgcttttcct ttgccagtac
    45301 aagagatagg aaagccgcgg cggcagttcg ggcaaaaacc acagccagaa atgtgataca
    45361 ccagcacgcg gtcgccctct ttaaaatggc ggcagccttg ccccatcgcc acaatctgcc
    45421 cgcacggttc atgaccgttg ataaatccct ggtataacgg tttatcgggt gccgccgccg
    45481 tggcacggtg ttgatgatag atatagtgga catcgcttcc gcaaatcccg gaggatttca
    45541 ttttgatcag tacctggtta atccccggcg tcggcaccgc aacttcccgc agatcgacgg
    45601 ttgaatttcc tggtaaataa gctgccagca tcgttttcat aaatccctca ttaacaatac
    45661 gattaatttt catccctgcc gcacccgcgc caggggcgtt aaggtttagc gtttcgcttt
    45721 actgcttcgc tgggtcagca agatattcgc cagcaccgcc accacgatga tgacgccgcg
    45781 taccacctgc tggaaaaagg agttaatacc gagcagcacc agaccgttac cgattagcgt
    45841 aatcaccagc acaccaagca atgtaccgaa cagggaaccg cgaccgccgg aaagtgccgt
    45901 accgccgacc acgaccgcgg cgatgacgtc aaactccaga ccgtttgcgg cacctgcgtt
    45961 accggaaccg aggcgtgccg ccagcaaaat gccggtcacc gccgctaata atcccgaaag
    46021 ggtaaagata agaatgcgca ctcgacgaac gttaataccg cacaactgcg ccgccgtggc
    46081 attaccgcca acggcaaaaa ccgagcgccc gaaggcggtt tttcggctaa tgaacacaaa
    46141 cagcgcaaac aacaccatca tgatcagcgc ggatactggc acaccgagaa attgtccgcc
    46201 cagccagtcc agcacctcgt tttcgtcaat cggcactggc agtgcgttcg tcataaacag
    46261 ccccattccg cgcagggcgc tccacagccc cagtgtggca acgaaacttg gtacgttaaa
    46321 cacgccgcgc agcaccccgg ccagcgttcc catcagcgcg cctaacagca acaccagcag
    46381 acaagcaatc gccaacggaa cttcaaattg cagcaaaaat gccaggcaca ccgagacaaa
    46441 agccaccatc ggcccaacgc tgacatcaat ttcaccggag ataataatca gcgtcatcgc
    46501 ccaggcggca atcccaatgg tggcggcatc gcgcagcacg ttcatctggt tattcaatga
    46561 gataaagcca ggcgcgttca gggagaagac cagataaaga atggcaatca ccaccagcaa
    46621 accgatctca ttaatatggc gactgacaaa ttgtttgagc gagacgctct tgccctgcgg
    46681 caatggtaat gacgaagcag acatcttcag gttcctcggt aaaattcatc agtgcacaga
    46741 cagaatggcg gacatcagct catccacatt gaccggcgag tgaaactcct gcgagaacgt
    46801 gccgtgctgt aataacagga tgcggtcaca caccagcggc aactcctcca cttcactgga
    46861 gataaacacc acgctttttc cttcggcagc cagctcacgg acaatacggt aaatctgctg
    46921 tttggcttcg atatcgacgc cgcgcgttgg ctcgtcgagc aacaaaatct ggctggaggc
    46981 atagacccaa cgaccgatca ccactttttg ctgattgcca ccagaaagcg tgccgatggg
    47041 tgtttcgcta ctggcagcct tgaccgtcat ccgctgcctc acctcttcgg tcaggcggcg
    47101 gatggtggac cattgcagca caccgttggt gctgattttt tgccgattgg tcagcactgt
    47161 attttcgtca acgcccagcc agggaatgat ccccgcttct ttgcggtttt ctggcgtata
    47221 gccaatgccg cgtttcagca tgtcgccgta atcggggcgc atgattttct cgccgttgat
    47281 aacaatttcg ccctgttcat acgtctccag cccaacaatc gccttcagca attcactgcg
    47341 ccctgccccc agcaggccag caatgccaag cacttcgcca cgacgtagcg taaagctgat
    47401 atcctccagc ttgggcttat ggcgtaacgc acggacttcc agtacggcct gatccacaat
    47461 ttcctgaggg gctaccggcg caatatcaac gtgatcacgc cctagcatta gcgacacaat
    47521 atgatgcgtg gaggtgtttt cgagcatcac atcgcccgcc acctgaccat cgcgcataac
    47581 ggtggcacag gaggcaatgc ggcgaatttc ttccatccgg tggctgacat aaatcaccgc
    47641 cacgcccagt gccgacatct ttttcaccgc gctgatcacc agttcaactt ccgcactcgc
    47701 aagcgaactg gtaggttcat caagaatgac cacgcgcggc tcgcccttca tcacccgcgc
    47761 aatttccacc agctgctttt gcgccgggct tagcgttgaa acaagttgtt caggactaac
    47821 gtcaacgccc agcgcctgta agcaacgttg ggcatcctgc gccatttgca ggtaatcaat
    47881 catgccgttg cggcggggcc actgaccgag gcagaggttt tccgccaccg tcagcccttc
    47941 caccagactt aattcctgat aaaccgcacg aacccccagt tcagcggcac ggcgagtcaa
    48001 cgtagcttcg tcaccttcca gtcgcgtctc gccaatccag atatcaccgc tatccgggcg
    48061 ttcgctgccg gtaagcattc gaatgagagt cgatttgccc gcgccgtttt tacctaacag
    48121 cgcacgaact tcgcctttat tgagcgtgaa gttaacgtta tccagcgcaa cgacgccggg
    48181 gtaacgctta tttcctgcca ccacttttgc taccgggact gcctctgttg ccgtgaacat
    48241 agggacctct gcgaatcagc gattagggca gaccatcaac gtgcgttgcc agccactgtt
    48301 taccatcttc cgttttggta tagagatcga ttggcacctg aatcactttt tcaccgtcgg
    48361 cttgtttatt gataaccttc aatgtttgcg cgaaaacagc attgcccatt ttcttaccgg
    48421 aaatatccac taccgctttc agcacctgat tgttttccag ctcctgagca atttcggttg
    48481 tcatatccga accgaaaaca gcaatttttc cggcctgatt ttgattacgt accgctttta
    48541 ccgcgccgag tgtcgcaccg cccgactccc ccataatggc gttgagatcc ggcgtggaga
    48601 taattagttt ttcaccaaca gaaatcgctt tatctaaaac agtcccttcc tgattagcga
    48661 caatttgcgc gccgggaaca cgggatttta atacttcttc aaatccttta cgtcgctgca
    48721 cacaaacttc aaaggcttcg caattgatga cagcaatttt cggctggtca attttgttgg
    48781 caataaaata atcggcggca gcattaccca gttttttacc aaattccagc ggatcgccga
    48841 ccagatacgc cgagacatat ttatcgacgc ccttttgatt aatacaggtg ttgtagcaaa
    48901 tcaccggaat acccgcttca ctggcgcgac gaacggtacg actactgcca ttttcagaca
    48961 ctgccgataa aataatggca tcgacattac gcgccacgag ggtatcaaca aaggtacttt
    49021 ctttcgaaat atcaccctgg gcgttagttt caattaactg tacctgtact gaagaatctt
    49081 ttgccgcatc ctgaacgccc tgacgcactc cagcgtaata tccctgggta tcaaggtata
    49141 ttgcgccaat ggtcatttct ttatccgcag ccctggcaaa tagtgcgcta cctaatagcg
    49201 tagccattaa taatagatta cgggttgttc tcattttttt aggcatagtg cctcctgtag
    49261 ggtttttatt aacaacggct tattctaatt attttgtgat gagcggcagc gcgtgccgct
    49321 caaatattac agttaatagg gtttagtgaa cgtagttaaa aataaaaggc atcacggtgg
    49381 tgaataatgc cgctttcggc caggccggat taatcgccgt caattcagcg cgagcagatt
    49441 catattgcgc aacctccccg agtcccgcgg acgccagcat cgccaccatc aggcattttt
    49501 ctttatgctg ctgttgtaaa tcgccataca gcgacaacag gtcaggttgt gaaacagcaa
    49561 agaaatccgc ctcaatactg gttttcgcca tctcttgcgc ccactgtttc atttcactaa
    49621 acagttgctg tgcgatttgt tgttcgccca gcagtcgcag cgccattcct tgccagaaga
    49681 gataatcaac cggctgatcg ttgtaataac tgtggatgtt aatggtgcga tcgccggtcg
    49741 ccgccagacg taaacaacgc gtcgcttcag tttcatcgcc ctgcgcgttg gcgcatatcg
    49801 cctgccagaa ccagatgtcg ttatcagttt gccccggtaa acggccttcg cttaaattct
    49861 ccggataatg cagcgcggca tgaagcagtt cgctggcctg ctgcggctgt ctggcatcaa
    49921 ggtgctgcca ggcgcgtaat aactggttga ggataaactg actggtgacc ttcccttccc
    49981 cgccttccca cgggtggaat ttgcgcgtgg cgagaatgtc cgccgctttg tctgcctgac
    50041 ccgtgagatg ccacaaattg agcaactctg ctgtcatgtc gtcgcgtttc agcgcaattt
    50101 ccagattatt ttccagacgc gccagtcgtt tctccggtgt ggctccgctt aacttatcaa
    50161 gcaaatcccg ttcgaaaagc agacgtgcat cctgcggcgc aagctgataa gcattatcaa
    50221 gataacgcgc ggccagctca taatcgtgtt gcttattcca cgcatggatc gctaacccgc
    50281 gccagccgtc ggcaaactcc ggcgacatct ctacgcaacg ttgccataag gtaatggctt
    50341 tgttgtagct acgcttgttg tagtagaagc aggccagtaa atgacgggca aaccagcact
    50401 cttcaatact ctccagcgcc gccacttctt ccagcgtatt cgggaaacgg acaaactgcg
    50461 ggaagacatc aatggctttt gcgaccagtt cgccgcgttc ggctttcggc aataaactcg
    50521 cctgcaggta aagcggcagc gtgcgctggt agtccagtgc gttcagcatc tctgccgcca
    50581 gggtgggcat tccccagtta atcagttgcc cggcggtcat cagagcgtta acgtcgcgtc
    50641 cctgacacag accgcgccac tgcgcgaggg ctgattcgct acgaccatcg aaccagttca
    50701 gccaccacag agtggcgttc agcggataat cacgcagcag tttcgcgcgc tgcaaacgcg
    50761 cgttgtcctg acgaccactt aacaccagca gcagattatg caggcaaagc acttcctgat
    50821 tggttgggca ggcacgaaga ctttgttggc aaaaatccag accagcgtcg aagttaccat
    50881 tacgcgccgc cagtcgtgcc aggccataat agccaccggc tttgctgttg ccgctccaga
    50941 ccgcacgcca gaaatcctct tcggcttgtt gatattgtcc ctgacgttcg taagcactgg
    51001 cgcgaatcaa actggcctgt ccgcactgcg gatttttgtt cagcgcatgt gcgcgtttca
    51061 gcgcctgagt ggcatacgcc accgcttgcg ggaaatcggc gcggttatat tccagcatcg
    51121 ccagcgccag gttacagcgg taatccagcg gatccagcac cacgccgcgc aggtagtaat
    51181 cgaacggtga acggctggcg tgatgatatt gctccagatg ctgaccgata aaccaggctt
    51241 catctgtact ggtaatgtct tgtgctgcga gtggcgcttt ggcgacgtcc ggcaacggca
    51301 actcttgcgg ctgatgttcc tgataactca ggacaatatt gccgtcggca tcggagagct
    51361 caatggtcag cctttcagga ttgataccgt gcaacacgcc ctggatggcg gtcgcaggca
    51421 tcagtgccac ggcatcatca agtaacgcgt tgcatttgcc gatttcgcgg atcgccaggc
    51481 ggtatccgtt caacggagag atggcataca gcccccactc aatcccccgc tcactacgct
    51541 ggagttttat caccgcatcg cgggaggcat tttgcaccat gcccaaagaa tgataaggca
    51601 ggaaatactg ctcaaaacgc ttctcttcgt aagcatcaag ccaggtaaaa tcaggctggt
    51661 tatcggcaaa aataccggtc atcagttcga tatacgggcc gttattgtcg gtcagactct
    51721 tgtcccacgc ctggccgaat tcactgtgtc cccagctcca ctgtttttta cctggcgcga
    51781 tatggtggtt ggcaacgtgt agcaaaccgc catcttcatc gtgacaccac gcgccaacaa
    51841 aatcgtactg tgatttttca gccatatatg aggttggaac gggcacattt ttatagcgag
    51901 aaatgtccac tccggcggag tagtccactt tgtagtaagt gccggtggcg atggggaaag
    51961 cggagacggc ccgtttgccg tgatcaaaca ccgccgttac atccggcggg aagacgctct
    52021 gatgcccttc cccccctttc actgccgggt tggcccacca caagaaatga cgcggcgtgg
    52081 cgttgccgtt atagacgcgg ctggcgattt ccaacgccgc ccgttcagga cgcagtgtga
    52141 aacctgtcat cacctgtaaa ccgtgcatcg gctccgtttc gccgacccac accgtctgtg
    52201 cgccgtcttc atgggcttcg agggtgaaat caacgggcat aaaggtggtc gggcgatggt
    52261 gttgcggcca gttaaactca atcccgccag agatccacgg tcccagcagc cccaccagcg
    52321 caggtttaat gacttcattg tgatagacaa aatcgcgttg tttcacttta tcccatgcgc
    52381 gatgcacacg accgcccagt tccggcagga tcatcacttt gatgtagtcg ttttccagcc
    52441 acaccgcctg ccaggatttc agggttttct gctcgctcag cgtatcggtc acgccgtagg
    52501 gataaaccgc gccggacgat ccctgataaa cgcgattttc caggaacatg ggatgtatat
    52561 cctgcggccc ggtttcatag gtcgggatct caacgcgctc ttgccacact tttactggag
    52621 tcatggtgcc ctcaatatta acaagacata ctgaattaaa agatttgtgg cagtgtattg
    52681 aacaatctgg caatgttttc gcggaataat cacgcaatta actaaacaag gtttagtgaa
    52741 gatgagagcc tgcattaaca atcaacagat tcgccaccat aacaaatgcg tgattctgga
    52801 actgctgtac cggcaaaagc gcgccaataa atcaacgctg gcccggctgg cgcaaatttc
    52861 gattccggca gtcagtaata ttttgcagga actggaaagc gaaaaacggg tggtgaatat
    52921 tgacgatgaa agccagacgc gcgggcatag tagcggtaca tggctgattg cgccggaagg
    52981 tgactggacg ctgtgcctga acgtgacgcc caccagtatt gagtgtcagg ttgctaatgc
    53041 ttgtttaagt ccgaaaggtg aatttgagta tttacagatt gatgcaccga cgccgcaggc
    53101 gctgctgtcc gaaatcgaaa aatgctggca tcgccaccgt aaattgtggc cggaccgcac
    53161 catcaatctg gcgctggcaa tccacggtca ggttgatccg gtgaccggcg tgtcgcaaac
    53221 catgccgcaa gcgccgtgga caacgccggt tgaggtgaag tatctgctgg aagagaagct
    53281 gggcattcgg gtgatggtcg ataatgactg cgtgatgctg gcgctggcgg agaaatggca
    53341 aaataattcg caggaacggg atttctgcgt gatcaacgtt gattacggca ttggctcgtc
    53401 gttcgtgatt aacgagcaaa tttatcgcgg cagtttgtat ggtagcggac agattggtca
    53461 caccatcgtt aatccggatg gcgtcgtcta cgactgtgga cgttacggct gcctggaaac
    53521 cgtcgcctcg ttaagtgcat taaaaaaaca ggcgcgggta tggctaaaat cacaaccggt
    53581 taatactcag cttgatcctg aaaaactgac aaccgcgcag ttaatcgctg cctggcagag
    53641 tggagaaccg tggatcacca gctgggttga tcgctctgcc aatgccattg gtttgagtct
    53701 gtataacttc ctcaacatcc tcaatattaa tcagatttgg ttgtacggtc gcagttgtgc
    53761 ctttggtgag aactggctta atactattat tcgccagaca ggatttaacc cgttcgaccg
    53821 cgacgaagga ccgagcgtga aagcgacgca aattggcttc gggcaattaa gccgcgcaca
    53881 acaggtgctg ggaattggct atttgtatgt tgaggcgcag ttacgacaga tttgatggcg
    53941 cgataacgta gaaaggcttc ccgaaggaag ccttttttca acagattaac cgtttcgctt
    54001 atgcgtaaac cgggtaacgt gcgcagatgt cgagaacttt acctttgatg cgctcgataa
    54061 cggcttcatc attgatgctg tccagcacgt cacacatcca gccagccagt tctttcgctt
    54121 ctgcttcttt gaagccgcga cgcgtaattg ccggagtacc cacgcgaata ccggaggtca
    54181 caaacgggct cttcggatcg ttcggtacgc tgtttttgtt gacggtgatg ttagcacggc
    54241 ccagagcggc gtctgcttct ttaccggtca ggtttttatc aaccagatca accaggaaca
    54301 ggtggttatc agtgccgccg gaaaccactt tgtagccgcg ctcgaggaac acttctacca
    54361 tcgctttagc gtttttcgcg acctgctgct ggtaagtttt gaactcaggc tccatcgctt
    54421 ctttcagagc aaccgcttta ccggcgatta cgtgcatcaa cggaccgccc tgaccaccag
    54481 ggaaaacggc agagttcagt tttttgtaca gctcttcgct accacctttc gccaggatca
    54541 ggccgccgcg cggacccgcc agggttttgt gagtggtggt agtaacaacg tgagcatgag
    54601 gaaccgggtt cgggtagacg ccagcagcaa ccaggcccgc aacgtgcgcc atatcaacga
    54661 acaggtaagc accgatgctg tcagcgattt cacgcatttt cgcccagtcc accacgccgg
    54721 aatatgcaga gaaaccaccg ataatcattt tcggcttgtg ttctttggct tgtttttcca
    54781 gatcggcgta gtcgatatga ccggtagcat cgataccgta aggaacgatg ttgtacagtt
    54841 taccggagaa gttaaccgga gaaccgtgag tcaggtgacc gccatgcgcc aggttcatac
    54901 ccagaacggt atcacctggt tccagcagcg cggtgtagac cgcaaagtta gcctgggagc
    54961 cggagtgcgg ctggacgtta gcgtagtcag cgccgaacag ttctttcgca cgatcgatcg
    55021 ccagttgttc aacgatatca acatactcgc aaccgccgta gtagcgtttg cccggataac
    55081 cttcagcata tttgttggtc agctgagaac cctgcgcctg cattacgcgc gggctggtgt
    55141 agttttcgga ggcgatcagt tcgatgtgct cttcctgacg tactttttcc tgctccatag
    55201 cctgccacag ttcggcatca taatcggcaa tgttcatttc acgctttaac atccgcatct
    55261 cctgactcag ctaacaataa aatttttggc ctttataggc ggtcctgttg gacaacggcg
    55321 aacagtataa ccgaatcatt gtgcgataac aggtcttgac aaaggaattt acgcaaacga
    55381 ttaccttcag gctacgcaag gctttggaga ataaagagct tgcaaccgga aacggatttc
    55441 ttttcaggtt tgtgatgcaa atttttcact tcatcacatt ctttctgaaa aacaccaaag
    55501 aaccatttac attgcagggc tattttttat aagatgcatt tgagatacat caattaagat
    55561 gcaaaaaaag gaagaccata tgcttgacgc tcaaaccatc gctacagtaa aagccaccat
    55621 ccctttactg gtggaaacgg ggccaaagtt aaccgcccat ttctacgacc gtatgtttac
    55681 tcataaccca gaactcaaag aaatttttaa catgagtaac cagcgtaatg gcgatcaacg
    55741 tgaagccctg tttaacgcta ttgccgccta cgccagtaat attgaaaacc tgcctgcgct
    55801 gctgccagcg gtagaaaaaa tcgcgcagaa gcacaccagt ttccagatca aaccggaaca
    55861 gtacaacatc gtcggcgaac acctgttggc gacgctggac gaaatgttca gcccgggcca
    55921 ggaagtgctg gacgcgtggg gtaaagccta tggtgtactg gctaatgtat ttatcaatcg
    55981 cgaggcggaa atctataacg aaaacgccag caaagccggt ggttgggaag gtactcgcga
    56041 tttccgcatt gtggctaaaa caccgcgcag cgcgcttatc accagcttcg aactggagcc
    56101 ggtcgacggt ggcgcagtgg cagaataccg tccggggcaa tatctcggcg tctggctgaa
    56161 gccggaaggt ttcccgcatc aggaaattcg tcagtactct ttgactcgca aaccggatgg
    56221 caaaggctat cgtattgcgg tgaaacgcga agagggtggg caggtatcca actggttgca
    56281 caatcacgcc aatgttggcg atgtcgtgaa actggttgct ccggcaggtg atttctttat
    56341 ggctgtcgca gatgacacac cagtgacgtt aatctctgcc ggtgttggtc aaacgccaat
    56401 gctggcaatg ctcgacacgc tggcaaaagc aggccacaca gcacaagtga actggttcca
    56461 tgcggcagaa aatggcgatg ttcacgcctt tgccgatgaa gttaaggaac tggggcagtc
    56521 actgccgcgc tttaccgcgc acacctggta tcgtcagccg agcgaagccg atcgcgctaa
    56581 aggtcagttt gatagcgaag gtctgatgga tttgagcaaa ctggaaggtg cgttcagcga
    56641 tccgacaatg cagttctatc tctgcggccc ggttggcttc atgcagttta ccgcgaaaca
    56701 gttagtggat ctgggcgtga agcaggaaaa cattcattac gaatgctttg gcccgcataa
    56761 ggtgctgtaa tttgatgttg ccggatggaa acatccggca acccttgacg cggtttaaat
    56821 tgccgcgtcg tcctcttcac cggtacggat gcgaatgacc cgtgccacgt caaagacgaa
    56881 gattttaccg tcaccgattt tgccggtttg cgccgtgcga ataatggtat cgacacaggt
    56941 atcgacaatg tcgtccggta cgacaatctc aattttcact ttcggcagaa aatccaccat
    57001 atactccgcg ccgcggtaca gctcggtatg gcctttctgg cgaccaaagc ctttcacttc
    57061 ggtcaccgtc atgccggtaa taccgacttc ggccagtgct tcgcggacat cgtccagctt
    57121 gaagggtttt ataatcgcat caatcttttt catgctattc cttgaaaagg tcgcctgtct
    57181 tttgatctgc taaacgtaac acataacgcc aattcattcc ttgaaatcgt ttgcatccag
    57241 ctcgtgtcgg gaaagcagtt tataaaattc tgtccggttg cgccccgcca ttctcgccgc
    57301 gtgggtgacg ttgcctttgg tgatttgcag cagcttacgc aaatagttga gttcaaactg
    57361 attacgtgcc tcaacaaagg ttggcagcgc cgtgttttca ccctccagcg cctgctctac
    57421 taatgcatcg ctaataaccg gagatgaggt cagcgccacg cactgttcaa tcacgttgac
    57481 caactggcgc acattacccg gccagctagc ggtcatcagg cgtttcatcg catcagtgga
    57541 gaacgcacgg acaaacggtt tatgtcgctc tgccgcctgg cgcaacaggt gatttgccag
    57601 aagcggaatg tcttctgtgc gctctgccag agccggaatt ttcaggctga caacgttgag
    57661 gcggtaatag agatcttcgc ggaattcccc gcgcgccatc gcttttggca gatcacggtg
    57721 agtggcagaa ataatccgca cattgatatc aatatcgcgg ttactgccca gcgggcgcac
    57781 tttacgctcc tgcaacacgc gcagcagttt gacctgtaac ggcgcaggca tatcgccaat
    57841 ttcatcgaga aatagcgttc cgccttccgc cgcctggaat aaaccttcgc gattgctgac
    57901 agcgccagta aacgcgccac gcgcatgacc aaacagctcc gactccagca attgctcggg
    57961 caatgcgcca cagttaatag caataaatgg tttgctgttg cgcgggctgg cgttgtggat
    58021 agcctgggcg aaaatctctt tcccggtgcc gctctgaccg ttaatcaaaa cgctgacgtc
    58081 tgattgcgcc accagccgcg cctgttccag caaacgcagc atcagcgggc tgcgggtgac
    58141 aattgcctcg cgccagcgtt catcggtggc tggcgcggat tgctccagcg catcgtcaat
    58201 tgcctgatat agcgcgtctt tgtcgacagg cttggtgagg aaactaaaaa cgccctgctg
    58261 tgttgcagca acggcatcgg ggatagaacc atgcgcagta agaataatta ctggcattcc
    58321 cggctgcact ttctggattt cagcaaatag ctgcataccg tccatttcat ccatccgcag
    58381 gtcgctgata actaaatcta ctttttcgcg attcagtacc cgtaatcctt cagcgccact
    58441 ttccgccgtg accacgctgt agccttcgct ggtcaggcgc aggccaagca gtttcagcaa
    58501 tcccggatcg tcatcgacca ataataaatg cgcaggttta tggctcatca ggagtgacct
    58561 catgggtgga tggcgcgggc ttttcaccct cgtgcggcgt atccggcgag aaatttccag
    58621 ccggtttgcg ggtcgagagc tggcgttcaa tatcagtcag gttttccagc ttgcgggtgg
    58681 tgagttccag ttgctgctga agaacatgat gttgctggcg caatgtatcc agctcgctgt
    58741 cgctggactg ctggagttta ctgtaacgtt ggcgctcttc cgccagttgc agttgcagcg
    58801 cctgaccgtc gcgccagagt tgatacagtg ggcgaacctg tgccgggatc tcggtactta
    58861 acgcttcaat acgcgcgacc agctggcggc gctcatatgg cgtaattttg gcgtcggcga
    58921 gcaaaatccc ttgtttaaag gtattttgcc agctgccgtc gtcatattgg cgagcttgct
    58981 gacgcgactg cgcaggcatt aaacgatcag cacaatccat cgcccgcagc cagtaaagcg
    59041 gattggtttc ggttgattta ccttgcagcg cccagatgtc gctacattca gtagaaagat
    59101 agtcagccag ttgataaacc ggaatttttt cttctgctgg cgtatcaatg gctggcttat
    59161 tgtgattctg cacgcacccc agcaatgcca gacatggcag ccctgccagc cacagccgtc
    59221 ggggcaataa tcgttgaaaa atgtgtcgca tattcaccag acttaaagcc tatcccagtg
    59281 ggcgtaattg ttgcaggcag tttggacatg gacagcgcgg agaaaccgga gcgtacacgt
    59341 cgtacgtgag gatttcgagc actgcccggg gccgaactgg caaataaaat agcctgatgg
    59401 gataggctgt tagcattatt tcgtgttttt cgacgacggc aattcaatgc ggaaacaaac
    59461 gtcttgcccg ctctcgtcga ccagatacag ttccccttgc atacggcgaa tacaatccct
    59521 ggcaatgctt aatcccagac cgctgccctt caccgcccct tttcgctggt ggcttccctg
    59581 aaaaaagggt tcgaagatca tggcgcgttc ctcttgcgga atgggcgtgc ctgtattgat
    59641 gacatcaata taaacccgcg caccatgtga actgctgcga aggcaaatgt taccggattc
    59701 agccccgtag tgcaccgcat tggagtaaag attatccagt acgctcatca gcagcattgg
    59761 ctccgccagg caagctgttg ctttgagatc gacgtcggta tgcatcattt tagcccgtgc
    59821 gggcaggcta tgagcagaaa ctactgtctc caccagcggt gctaactcaa cattctccag
    59881 ttccaccgca ctgtccgcct gtttacggtt gtaatcaagc agttgttcga tcagtttttg
    59941 caaattgcgg ctgctgctat caagaatgct caccacctct ttttgctctg gcgtaagcgg
    60001 cccaacaacc tggtcagcca gtaattcagt gccctcgcgc atactcgcca gtggcgtttt
    60061 taattcatga gataaatgtc ttaaaaattg atggcgttgg gattccagcc atgacaggcg
    60121 ctcacttaac caaagaatac gttgcccaac cgagcgtaac tcgctcggtc cactgaacga
    60181 gacgctattg cccagagaac gcccttcccc cagacggttg atcatgcgct cgatattttt
    60241 caccggacca ataatcatcc gcgtgaaaag cagtaccatt accagactca ccagaaatag
    60301 caccagcgat tgccaaccaa aatattgccc acgttcggcg atttcacgct gaagttgctg
    60361 cccacgagag aacaccactg tgcgcgtggc ctgcaccatt tcggtattgg cactggcaaa
    60421 ggcttcaaga cgcgcggcgg cggcagcatc gggaccactg ttgttacact gaagttgggc
    60481 cagattgttc aaatcctgac gcaatgcctg gtaaagttta tcgtctggca gtacgcctgc
    60541 gtgggcatcg agcatttcgc tgtaacgctt gcgctggctt tgataaacct tcgcaagcgt
    60601 aggatcgtcc agcacgcaat actgacggta gctacgctcc atctccagcg ctgcgttggt
    60661 cattgcttcg ctgcgccggg catcgataag cgtggtgcgg ttgaccagcg ccgcctggtc
    60721 gctaagcgca ttcaggcttt gccaggcttg ccatgccagc accaacaggg gcagcagaat
    60781 cagcaaaaat gccagcatta ccagttgtcg taatgagcgg ggaaaaacgg gccagcgttt
    60841 caaggtgtta ctctcgtcag acgcgaatag cctgatgcta accgagggga agttcagata
    60901 caacaaagcc gggaattacc cggctttgtt atggaataag gcggtgccta actcgacgtt
    60961 tcgcccgatg gttgatatag ctacgctgat atcagaagtt ggacggcagg caccttgttg
    61021 tgcgtcattc gtattttatg tagcacgtcc cgaaggggct gacataagtc ggtgaatgag
    61081 ccactgctta ctattatgca gtaactgtgc caataaagaa aatagtttgg taacgtactg
    61141 attatacgct gctttgaggg gtttatggct tctccgctgt ttgaatgtaa atcagccact
    61201 gtgtcgctaa aaagagacaa cttaagataa acttattaga ttacattaaa atcaatcatt
    61261 taagtgtcgc cagaaagcga cacggtaaac cacccattgt cgtgatttac agacacaaaa
    61321 aagctccctg agttgggagc ttataatagc ggttggtatt taatgccgga tgcggcgtga
    61381 atgccgcatc cgggctgcaa aaccaatggg ctgacgactt accccaactg cttacgcgca
    61441 ttgcggaaaa tacgcatcca tgggccatcc tcgccccagt tttccggatg ccaggagttg
    61501 ctgacagtac ggaaaacacg ttccgggtgc ggcatcataa tggtgactcg accactttca
    61561 gtcgtgactg ccgtaatacc gttcggtgaa ccgttcgggt tagccgggta ggtttcagtg
    61621 actttgccga agttatcgac atagcgcagt gccaccagcc ctttgctttc cagtgccgcc
    61681 agatgcgccg catcacgcac ttccacgcgc ccttcaccat gagagacagc aatcggcatt
    61741 tgcgagccca ccatcccctg caacagcaga gacgggcttt gggtcacttc aaccaggctg
    61801 aaacgcgctt caaagcgatc ggaggtattg cgcacaaaac gtggccacaa ctcgctcccc
    61861 gggatcagtt cacgcaggtt agacatcatc tggcaaccgt tacatacccc cagcgccagc
    61921 gtttgcggac ggtggaagaa ggttgcaaac tcatcgcgta cacggtcatt gaacaggatt
    61981 gacttcgccc aaccttcacc ggcacccagc acatcaccgt aggagaaacc accgcacgcg
    62041 accagggcgt ggaaatcttc caggcccgtg cgtccagcca gcaggtcgct catatgcaca
    62101 tcgattgcat cgaagcctgc acggtggaag gctgccgcca tttcaacatg cgagttcacg
    62161 ccctgctcac gcagtacggc aactttcggg cgcgcgccag tggcaataaa cggtgctgcc
    62221 acatcttcgt tgatatcgaa cgacagtttt acattcaggc ccggatcggc gtcgtttgat
    62281 ttcgcctggt gctcctggtc agcacactcc gggttgtcac gcaggcgctg catctgccag
    62341 gtagtttctg cccaccagac acgcaacgta gtgcggcttt cgctgaatac agtctgcccg
    62401 ttggcggtaa tcacaaaacg gtcaccggaa accgcctgcc caacataatg gacgcaatca
    62461 gcaagcccat gctgtgccag tacggactcg accgcttcac ggtcagcggc acgaacctga
    62521 atcaccgcac ccagttcttc gttaaacaac gccgccaggc gatcgtcacc cagagagccg
    62581 atatccgcat caatgccaca atgaccagca aaggccattt ccgccagtgt gaccaacaga
    62641 ccgccatcag agcggtcgtg atacgccagc agcttacgct gtgcaaccag cgcctgaatc
    62701 gcgtcataga agcctttcag ttgcgcgaca tcgcgtacat ctgccggttt gtcgccaagc
    62761 tgacgataaa cctgcgccag cgccgttgcg cccagcgcgt tattgccttt gcccaaatca
    62821 atcagcagca gtgcgttatc ttcggtagaa agctgtggcg tgatggtgtg acgcacatct
    62881 tccacgcggg caaatgcaga aatcaccagc gacagcggcg acgtcatttc gcgctcttcg
    62941 ttaccttcct gccagcgggt tttcatcgac atagagtctt tacccaccgg gatcgtcagg
    63001 cccagcgccg gacaaagctc ttcgcccacg gctttaacgg cttcatacag gcccgcatct
    63061 tcaccagggt ggcctgccgc cgccatccag ttggcggaaa gtttaatgcg tttgatatcg
    63121 ccaatctgtg ttgcggcaat attggttaac gcttcaccga ccgccagacg ggcagaggcg
    63181 gcgaaatcca gcagcgcaac cggcgcacgc tcgccaatcg ccatcgcttc accgtagtag
    63241 ctgtcgaggc tggcggtagt gaccgcgcag ttagcgaccg gcacctgcca cggacccacc
    63301 atctgatcgc gcgctaccat gccagttacg ctgcggtcac cgatggtcac caggaaagtt
    63361 ttctccgcca cggtcggcag atgcagtacg cgtttcaccg cgtctgcaat ggttatccct
    63421 tcacggacca gcgcatcgcc cttcgctttc agcgtttgta catcgcgggt catcttcggc
    63481 gttttaccaa gcaggacgtc cagcggcaga tcgatcggct gattatcaaa atgacgatcg
    63541 tgcagagaaa gatgcagttc ttcggtcgct tcaccaatca ccgcgtaggg tgcgcgctca
    63601 cgcttacaca gttcgtcaaa cagcggtaat tgatcggcag caaccgccag cacgtagcgt
    63661 tcctgggatt cgttacacca gatttccagc gggctcatgc ctggctcgtc gctcagaatg
    63721 tcgcgcagtt caaatttacc gccacgcccg ccgtcgctca ccagttccgg catagcgtta
    63781 gaaagaccgc ctgcgccaac gtcgtggata aacagtattg ggttggcatc accaagctgc
    63841 cagcaacggt caatcacttc ctggcagcga cgctccatct ccgggttgtc gcgctgtacg
    63901 gaagcaaagt cgaggtcggc atcagactga ccagacgcca tggaagacgc cgcgccgccg
    63961 ccaagaccga tgttcattgc cgggccgccg agaacgacca gcttcgcgcc aacgttgatc
    64021 tcgccttttt gtacgtgatc ggcgcgaatg ttgccgatcc cgcccgccag catgatcggt
    64081 ttgtgataac cgcgcagctc ttcgccgttg tggctgttca ctttttcttc ataagtacgg
    64141 aagtagccgt tcagtgccgg acgaccaaat tcgttgttaa acgccgcacc gcccagcggg
    64201 ccttcggtca tgatgtccag cgcggtgaca atgcgctcag gcttaccgaa atcttcttcc
    64261 cacggctgtt cgaagccagg aattcgcagg ttggaaacgg agaaaccaac caaacccgct
    64321 ttcggcttcg cgccgcgccc ggtggcacct tcatcgcgga tttcaccgcc ggaaccggtc
    64381 gccgcgcccg gccacggaga aatcgccgtc gggtggttgt gagtttcgac tttcatcaga
    64441 atatgcgccg gttcctgatg gaaatcatag cggcccgttt cgtggtcagc aaagtagcgg
    64501 cccacttcag aaccttccat tacggcggcg ttatctttat aagcagagag aacgtgatct
    64561 ggcgtggttt cgaaagtatt tttgatcatc ctgaacagcg atttcggctg ttgttcaccg
    64621 tcgataatcc agtcggcgtt aaaaattttg tggcggcagt gctcagagtt cgcctgagcg
    64681 aacatataca gctcgatgtc gttcgggtta cgaccaagct ttgtgaaagc atcctgcaga
    64741 tagtcaattt catcttccgc cagagccagg ccaagacgca ggttagcgtc gatcagcgcc
    64801 tgacggccct gccccagcaa atcaacgctt gtgaccggag tcggttgatg gtgggcaaac
    64861 aactgctctg catcatctaa atcaaaaaag accgtttcca tcatgcggtc gtgcagttca
    64921 gcggtaacct gctgccattg ttcattggtc agcgtaccgg cttctatata gtaagcaacg
    64981 ccgcgctcaa ggcggtttac ctgttgtagc ccgcagttat gggcaatatc ggtcgctttc
    65041 gaagaccagg gagagatggt gccaggacgc ggggtcacca gcaggagttt gccttgcggg
    65101 gcgtggctgg cgagtgccgg gccatatttc agcaggcgtt caagttgtgc gtgctcatca
    65161 tcgtttaacg gcgcattgag gtcagcaaaa tggacatact cggcgtaaat attgtgaacc
    65221 gggagcctgg cagcctgaaa acgtgccagc agtttgttga ttcggaatgc cgacagtgca
    65281 ggcgaaccac gcagaatttc catcataagt ctctcgtctt ctaagctttc ggtgtaccca
    65341 agggggggaa acgggcgtca ttataaagaa tctgatgcgc tgacgaaacc gtttgcgtgg
    65401 aaataaaatc accatcgtga attagcaacg cgtgccgcca atggctgtaa taagttgcca
    65461 tctggcgcag gtttacgcaa aatgccgctc atttatgagt aaacctttca ctattattac
    65521 gttttttcaa gctgggacgc gcacgacaca gagaattaac taattgaaaa aattaaagat
    65581 taattatctg ttcatcggca ttctggcact gctgctcgcg gtcgctctct ggccatccat
    65641 tccctggttt ggtaaagccg acaaccgtat cgccgccatt caagcgcggg gagagttgcg
    65701 tgtgagcacc attcatactc ccctgactta taacgaaatc aacgggaaac cttttggcct
    65761 ggattacgaa ctggcgaaac agtttgccga ttacctcggt gtaaaactga aagtgaccgt
    65821 gcggcagaat atcagccagc tgtttgacga cctcgataat ggtaacgccg acctgctggc
    65881 ggcaggactg gtctataaca gtgagcgggt aaaaaattat cagcctggcc ctacctatta
    65941 ttccgtgtca caacaactgg tttataaagt gggtcagtat cgcccgcgca cactgggcaa
    66001 cctgacggcg gagcaactca ccgttgcacc gggtcacgtg gtggttaacg atctccaaac
    66061 cctgaaagac acaaaattcc cggaattaag ctggaaggta gacgacaaaa aaggctctgc
    66121 ggaattaatg gaagatgtca tcgaaggaaa actcgattac accattgctg attctgtcgc
    66181 catcagcctg tttcagcgcg ttcacccgga gctcgccgta gcgctcgata tcaccgatga
    66241 acaaccggtg acctggttta gcccgttaga tggcgataat accctttccg ccgccctgct
    66301 cgacttcttc aacgaaatga atgaagacgg tacgctggca cgcattgaag agaaatacct
    66361 ggggcatggc gatgattttg attacgtcga tacgcgcaca tttttacgcg ccgtcgatgc
    66421 ggtactgccg cagttaaagc ccctgtttga gaaatacgcc gaagaaattg actggcgttt
    66481 gctggccgct attgcttatc aggaatcgca ctgggatgca caggccactt caccgacggg
    66541 tgtgcgcggc atgatgatgt taaccaaaaa taccgcgcaa agcctcggca ttacggatcg
    66601 taccgatgcc gaacagagca tcagcggtgg cgtgcgttat ttgcaggata tgatgagtaa
    66661 agtgccggaa agtgtgccgg agaacgagcg gatctggttt gccctcgctg cgtacaatat
    66721 gggctatgcg catatgctgg atgcccgcgc cctgacggca aaaaccaaag ggaatcctga
    66781 cagttgggct gacgtaaaac agcgtctgcc tttacttagc cagaaaccct attacagcaa
    66841 gctgacttac ggctacgctc gtggacatga agcctacgct tatgtcgaaa atattcgtaa
    66901 gtatcagatt agcctggtgg gttatctgca agagaaagag aagcaggcta cagaagcggc
    66961 gatgcaactg gcgcaggatt atccggcggt atcgcctacg gagttgggca aagagaaatt
    67021 tccttttctc tcgtttcttt cccagtcgtc atcaaactat ttgacccatt ctccctctct
    67081 gctgttttcc aggaaaggga gtgaagagaa acaaaattaa tccgtcgagg attgcgcttt
    67141 tttctgcgct ttaatttcct ggcggcgcat gcgaaagaag tcactgagca acgccgcgca
    67201 ctcatccgcc agtattcctt ccgtaatttc cactcggtga ttcatacccg gatgatgcag
    67261 cacatccatt aaagatcccg cagcgccagt tttcgcgtca cgcgcaccaa agaccacgcg
    67321 accaatgcga ctgtggatca tcgctccggc acacattaca catggttcaa gcgtgacata
    67381 caacgtggcg tcgatcagac gataattttg catcaccaga ccaccctgcc gcagggccat
    67441 gatttctgca tgtgcggtgg gatcatggcg accaatcggg cggttccagc cttcgccgat
    67501 tacccgattg ttatgcacta ataccgcgcc gaccggcact tcccgctcat cccaggcacg
    67561 tttcgccagc gtcagcgcgt gacgcatcca gtattcgtgg ctaaattcga cttcagacaa
    67621 aaagaaaact ccggttataa aagcgcggcg cattatacac ggacgctatg ctttactcta
    67681 ttccagttgc tggagttcac cgcgcggggt tactcgccaa cgatgctgac agaaataaag
    67741 cagcggattg tcctgattac tgtcgctata gccactgtac agccgcagcg gagtgccgat
    67801 tttgcgctcc agttgtgcga ccttttcatg tcccagacaa cgcatcgtca atacccaacc
    67861 accatagcca cgctgaattt ggctggcgat aagattaacc cgcggcagcc agggtgtatc
    67921 gaaataaacc gcttcaacca gcggctgcgg agagccggta atcaaccaga tatcagcatc
    67981 ggaacttaac aggtaggtgg ttaatcgctc ctgaaccagc ggaaaggcgg taacattgtc
    68041 gcgaaaccag cgcacgaaat cggcctgcaa cgtctgtaaa cgtgcttcgc tgtgaccaaa
    68101 agtgcacccc cacagaagca gactcatcgg ccagcgtgcc gcacgacctt ttatcaataa
    68161 cgcaatggct ataatcggta acaacggcag gacaagtaac gcattcagcg gttggcgacg
    68221 cagtaaatag cgcagaaaac tgccgaacat atcctgctga tgcaatgttc catctaagtc
    68281 aaaaaacacc acacgacgct cgtgagttgc caaaccatac tcctctggac tgaaacatcc
    68341 tgattaattt tcgctactac atagcctaac agatagatca tcacttttcc ggcaacaatc
    68401 gaccgcatca tggctaactg gaatttttaa ttcatggcaa ttagcggcaa tggaatataa
    68461 aattcactcg cgtgtctctc atattgcctg ttgtcgccac tatttcatcg cgaggaaaga
    68521 tatgaacggc ttacttcgta tccgtcagcg ttaccagggg cttgcccaga gtgataaaaa
    68581 actggcggat tatctgctgc tacaacctga tacggcgcgc catttaagct ctcagcaact
    68641 ggccaacgaa gccggagtca gtcagtccag cgtcgtgaag ttcgcgcaaa aactcggcta
    68701 taaaggtttt ccggcgctta agttggcgtt gagtgaagcg ctggcaagcc agccggaatc
    68761 gccctccgtg cccattcata accaaatccg cggtgatgat ccgttacggc tggtcggcga
    68821 aaaactgatt aaagaaaata ctgccgctat gtacgcaacg ctaaacgtta atagtgaaga
    68881 gaaactgcat gaatgcgtaa caatgttgcg ctctgcgcgg cggataattc tgaccggtat
    68941 tggcgcttcg ggtctggtgg cgcaaaactt tgcctggaag ctgatgaaga ttggcttcaa
    69001 tgctgccgca gtgcgcgata tgcatgcgct actcgcaaca gtacaggcgt cgtcccctga
    69061 cgatctgtta ttagccattt cctacaccgg tgtacgacgc gagttaaacc tggcggcaga
    69121 tgagatgctg cgagtgggcg gaaaagtgct ggcgattacc ggctttactc cgaatgccct
    69181 gcaacagcgt gcttctcatt gcctgtatac cattgccgaa gaacaagcga caaacagtgc
    69241 ttcaatctct gcttgtcacg ctcagggaat gttaacggat ttgctgttca ttgcgctgat
    69301 tcagcaggat ctggaactgg caccagaacg tattcgtcat agtgaagcgc tggtgaaaaa
    69361 actggtctga gtaaagaatg cgcgtataat gcccgcccgg tttgtgttgt tttgagagtt
    69421 tccttatggc gttgttaatc actaaaaaat gcatcaattg tgatatgtgt gaaatcatat
    69481 aaaaacacat ttattttcat agtgatacag caatgtgtta ttgaatgtgt tgttggattt
    69541 atggtgcaaa aaaaattttg cataagttca gcaacacaaa gatcctcccc ctttctataa
    69601 cggttcattt gagactcccc cttgactcac tcttcgaaat tgaagaccat cacctgtaca
    69661 taattacagc catggataat attatgcgca gaaaagagtc aaacgttagc agtttgccgg
    69721 aactcaccaa ttttgaggta agctattctc tggttaccaa tgaagtttac ttgtctgcat
    69781 ccttcactga taacatggcc tgtataccaa actggccctt acaagaattt ccggatcaac
    69841 taatatgtat ttctcgagca aaagctgttg ccctaatcga agagctccag aagaccataa
    69901 actatatgga tgcaggaata gatcgaagtt caggtagcct tctccagtag ctgacgagtt
    69961 ttataataat ttgatatata tatatcgttc aatattagcg agtacttata atgagtgaat
    70021 ttagtaatct tttaaatagc attcctggat ggttatcctc ctccctcacc gctctagttg
    70081 gtactcttat tggcggatgg tttaccctaa aaggagttac ccaacaagct aagctgtcta
    70141 aagtagagac agaaagagaa tcccttgaat tgcaactttc agtattaaaa ggtgttaagg
    70201 gagaagtttt caccctaatc aatttatata ataaaaggat gaaaatacac attgataata
    70261 tcaaacccgg acagatgtta attctcactt tccctgtagg ggatgataat ttcacattct
    70321 atgagcaaaa tgcgaatgtc attgcaaaac taaatgattc tgccagagac tcaataatta
    70381 atatctacac atattcacgg tcattaatac aatcatttaa aggaaacaac aaactcattg
    70441 aggattatga aaaaatcctt attggtatgg ctgacaataa caaagataaa accatgtata
    70501 agagattaca tgatgcaaaa atagatgtaa tggtagatta tgctcaaggg ataaaaaata
    70561 ttgatgcaga gctaagagat gctgtcaata agggattcaa tattatcgac caagaagtaa
    70621 agtcgctaca aatgaaatta aacaaattag cttcatagaa tgctccactc cctgttcaat
    70681 atttttacaa gcttatcagc cagccgcagc acgttcctgc atacgacgtg ctacggtttc
    70741 attcatctcc gaccggaaac ttcttataca aagtcgatac gccaacatca taaatgatcg
    70801 ccacattcta gcgaggaact cctgatgcaa ttagtcgccc ggcctgcgcc cattgttctg
    70861 gtgtttgtta ctatgagtgt gcagaaaaat atcgtataat gctctcctca gcatccgtaa
    70921 agtatcacga acttagcttt taaataacaa acaaccagat tttacacttc attcaattta
    70981 ggactaatat ggtcgaaagt aataattaag gagattatcg tgttatctta cttaatagca
    71041 attcattttg ttttacttgg gaattcttct gatttaaaaa gcttttggaa atctgaagta
    71101 atacggcggg agtatatgga tatctggagg cttttcagag aaaaaaaaca gcgtaacagg
    71161 aatttccttt tctggtggcg gctggctaac gaaatgtata ttaatggtaa taaattacat
    71221 aagaaagctg ctaaaaagtt aaacaataaa ataattaaca aatttggttg cgagattgga
    71281 ctgggagcaa aaattggaaa agggctaaca attccccatc acgctggaat tgttgttcat
    71341 tttgctgttg atgctggtga aaatctggtg ttacgacaga atactacgat tggacagata
    71401 gatggtgaca tacctggttc aagagtaaaa attggtagta acgttgatat cggagctaat
    71461 tgttgcatca ttggattatc acgtaaaatt ggggataatg taaaaatagg cgcaatgtct
    71521 tttataaata aagatatacc atcgaactgc acatatataa ctaaaaagag cggtgttgta
    71581 ttgtataaat agagcacata aagccatcga tatttctatc gatggctttt tctttttatt
    71641 gtggggcgac tggccactca atatccgctg ctactgttgt atcaacacga ttcaacaata
    71701 cccgatatgt cttccatgca gccagtaatg atgcctcttc ttccgtcgca atatccaaat
    71761 ctacagcatc ctgaagtggc gcaatatgct cgctagctac ctgcatcagg ctgttttttg
    71821 tttcttccgc ctcctggctc cgaaacagtt tttctgcttc tgcatctttc acccaggatg
    71881 taccgttcca cttctgaaac tccccttccg gggataacca ggtgacattt tccggtaatg
    71941 ggccaagttc agaaataaat aacgcgtcgc cggaagccac gtcatagacg gttttacccc
    72001 gatggtcttc aacgagatgc cacgatgcct catcagtgtt gaaaacagcc acgaagccag
    72061 ccggaatatc tggcggtgca atatcggtac tgtttgcagg tagacctgta tgaggtggaa
    72121 tatatgcgtc accgtcacca ataaattcat tagttccggc cagcagatta taaattttga
    72181 tggttcgtga ttgttcactc attctgaatg ccattatgca agcctcacaa tatagttaaa
    72241 tgcgatgttt ttgacggtgt tttccgcgtt accagcagcg ttaacggtga tggtgtgtcc
    72301 atgtgagcca atcgcgacgg agtgcgtgtg tgcaccaata ccgacagtgt gctgatgaga
    72361 accaatacct acagtatgag aatgtgagcc agaggaacct gttgttgctg atgtatattg
    72421 agttgccaga cgcccagatg tcagtggaat tttccccccc tggtttgcac catctgatgt
    72481 tggtattgaa tgcgtgtgac tcccggcgtt actggtagtt ttggtcccat agtcaaatag
    72541 tgaggttgtt ttcgtcccgt aatcaaacga cgatgtggtt ttcgtaccca aatccgtact
    72601 ggatacgctg gcgctgtggg tgtgcgattt aatgccgtcc tgttcctgag acaatacggc
    72661 acgaccactg gcgggcttgc ccttaatcgt ccagccacgc atatcaggga tcacgcctga
    72721 cggataagcg gctgcaagtt tcgggtatgc agacttgtca aaagtctgtc cctgcatcag
    72781 agcataacca gatggaactg tatctgatgg ccacgggatc ggggcaccta caggaaaggt
    72841 cgaattatct tccagaccaa gatatttaag gacatcagca acagagcttt ttgccaaaat
    72901 atctcgccca acctgtgtca ggtcagccaa attagcggta tcttgattgg taaaatacgg
    72961 taatttgttt tcggatgctg aaagattagc aagtgcggta agtatgacat taattctctg
    73021 atagttctta tctaatgcag acgacatttt tgcgataaag ccggcctgat cgccatcatc
    73081 ctgtacatcc agtccacttt catttgctgt atattgtgct attgcagatg caataaagct
    73141 ggcctgccga ataaccttat tgacttgcgc actggatgct ttccctgctg taaatccgga
    73201 taaaagcgca ggcaacgctt cccattcctc ctgcgatata acattggcat ttcgatccgt
    73261 tgcaaacgct ttaaagtcat ttttcgccat cagagtaata ctccccatgc tcctacatca
    73321 aaaccactga tgaattcgtt atccatatca aaaccaaaaa attttgagcc ttccgatggg
    73381 gtttccaccg aaggtgtttc aatgccaccc gcccataccc cggcggcttt tactgtgaga
    73441 tacccctgtt taattgccgc aattaactca cgcgatacat ctgaaatacc agtatcagga
    73501 aagacccaga ccgatatcgt catgtcctgg ttatcgacta tctgcattcg cagcccggat
    73561 cctgctgttg cagcgtcaag aattgccgga agcgaatcat tccgtccatc ccagttatta
    73621 atcgcaatct tcgctttaag aatgacacga taagtttcat cgctgaggta catgtatccg
    73681 gaatcaggat cgtatggtcc ctgccatacc ccctgatcat atccaagccc gtcggtatcc
    73741 cagctgaaat agacacctga gataggctgg ctgacaacac ggctacgtcc gatccacaat
    73801 cccagaatgt caagttgcac accaaccgca gagtcaatat caaatgcagt aatcagccct
    73861 ctggtggccg ccgcaacatc aataagcggc cgggtcatca gatcaacatg tgcaagaaat
    73921 ttaggtttgg tggcgtggta gttcgtgatt agttcggtgt atttgctcat gactccaccg
    73981 ttataacgat attttccggg gtacaggaca cagattcgtt gtatctgata tcaatgtttg
    74041 atgacgacaa agcccccggg gatttcccaa tcgtcagttc ctgaatatcg taatagcgtg
    74101 cattcccgcc actcaccacg ccaagattcg ccggtgagta aatgcgactt aaaaggaccg
    74161 aatcaccaat catcagacta ttgatatagt cggaaatagc ctgctggatc tgctgcccta
    74221 tctgtgaggt ataacccgta aaaacttgta atttgatccg ggcataaaca ggtacatcac
    74281 tggaacgcga gaacttgatt acatggggat tgccgtattt atccggaatc gtaacggatg
    74341 ttgtaccgtg agtggctgtc ccctgacctt tattccctct gatagcctga gcaatatccg
    74401 tcacatcacc gccatccaca atcacagcaa cagagtgtgg cggtaacccg ttaccgtcct
    74461 ccgaaccagt atcattttca tagagtttgt ggcgggttac accggtaaca ttagaaacag
    74521 caccatccag tgcttcaaat ggggttattg atggcaacgc aacactctgc gactggcgga
    74581 tacgtaactc cgcgtcagtt tctgccggag tgcccacagt agctgcagca ggattggtta
    74641 ccgaaaccca gccacgggtt ggcgtattaa tttcagtgat agttccagcc agcgccgcca
    74701 ctgcaccact gacggaacat gttgcggtca ccatcactgt accatccacg ccgaccacca
    74761 ctgaagcagg caaacgccat atcacattat tactgtcttt cacgctgcca ttaatgatgg
    74821 ttgttccggc agttcctgta agaagcaaat caaccgtaga attcgtcgcg cctttacgtg
    74881 aaataccatt tattttcacg ttactggtca gtgcggcccc atagccggtt gctggtgaaa
    74941 aacagttgta gacagttatc gccatattat tggcatcatg aatcgccagc gccatcagag
    75001 ccaccatctg accgtcttta ctgtccggtt cgaggtaggc atcactacca taaatctgct
    75061 gaaaatagct aatcagggtg ctgagtatcg tctgataatc aggcgcactg atcccctccg
    75121 cggttacctt tgcagataaa ccgagagaat caaggttcag agccattacg cctccgatgt
    75181 aacagtcgtt attccataga gagtgtcgat ttcagcggaa aacatgacac gtcgggtcgt
    75241 ggtatccacc gtcgtattga aagagaggat tgatttaacg ccctgcgttt cgaggatgcg
    75301 cttacggatc gccaggttgt aagtttctgg tttttgcctg cccagcacgg actggatcca
    75361 cggtgttccc tctgtggtat caagaaacca ttgcccatac cacaattcga atcgcgtttt
    75421 cacagcctgc gctacggcct caggcgagtt aatcagccag gtgtcatcac cgctgccaaa
    75481 ggtgtaatcg ccatcggcgt cttcacgtct gtatcgcatc agtttacccc gtcggtactg
    75541 cttccaccac gctgaacacc accataagtg tgtgtatcat cgattggctt gccattagcc
    75601 ttcacgctcc ccaaaaactc aacagcacca gtgatttttg aagccacacc agaaaccaca
    75661 gaccccacca tgccccccat ccaggttaac aggccatgaa tggttacttt ctcagaaaaa
    75721 tcagccagag gggcaaccac atcaagacca cccggagcga caattttaat tttcccggta
    75781 tcaggattaa gctcaaaata ggtgctgcca tcgtcactac gcaactgtgt ggcactggta
    75841 ttaataccgc taatcttcct tgcctgcgac tggggaccga caatacaaaa cgcatccgat
    75901 aaatcatgca ttctgtcatc aaccggctcc tgtatcccgc cgttctgcca ccagaaatcg
    75961 atgcagcgat cggcaaaaat caccaggcat tcatcgccgg cttccaccgg gaaagttaac
    76021 gtgcagccgc cgccgcgcgg aaatatcacc ggcacatcca ccagcagcgg gtaatttttg
    76081 gtaacgcggt tgccgtcgtt gtcagtttca accgaacgga tcgcaggctg cacaaccgct
    76141 gtcaccgcgt caggatcgaa tgactggacg atgccaggca aagcaacacg gatctggttc
    76201 tttgttgtgt cccgctctga tttgaatgtt tcggcaaggt cgccgctgcg ggtctggtca
    76261 gataatgcca tttggtaggc tccagaaagc aaaaaacccg ccgggtggcg ggttttatta
    76321 attacttaac aggcttaact tgcagttttt ttggtatcaa tcataccagc aataagtgac
    76381 tgcaaatcct ctgcatcctt gatggtacta caagcatcaa tccgattaac gtgcttatca
    76441 agattataat cagcaatgac tcttcggtga tgtgcggttt tgagcttaag cccaaccttt
    76501 ttggcagaag ttcggtcaag actatgtgct agagctgcat tctcgccgca aagatattca
    76561 gaaagcctcg catgtacccc accgggtaac ttttgccctt tctcatcttg agttgggata
    76621 tagccatccg tgagcaataa cgctgaatga tacatcgtat aatacgctct acttatcgca
    76681 ttacgcgtcc actgttcacc gctattctca atagagcttt tagccatctc cagaaagcag
    76741 ttaatcgcta caggcatcag ttcaccgcct caaagtaagc gacactgcat cccgtttcta
    76801 gacctctttc aatcatctca tcaacgacct tatcgttaag atatgaaaga tactctgcgt
    76861 cttcagtttc gatgccaaca gaaaaagaac cataaccctc accatcggta agcagactgg
    76921 aggttttagc acggagtgat tcgctttcca caatgtacat caatacatct gcagcttttt
    76981 tcaaaccgtc actcattgga ccgcctaagt tgctcaaaac tacagcggtt tccaatgcag
    77041 atacatgctt ttctttttca gtttgaccaa atttctcaag cttagcgata gccatatcga
    77101 gaatctcccc atcagcccag aaggccgcaa aatttaaagc atgttcgacc atgcagggga
    77161 agtcgtagtc gagagcccga gtaagcaact ctcgccgttt agagtaaagc ccaaggttgc
    77221 ttagtgcgct agaataattg ttccaagtaa caggatctgt cgggcatatg gctagagatg
    77281 tctcaatcgc ctcgatacct cgctccactt tgccatccag caacagaatc agcccttcaa
    77341 gtgctagaga ttgatacctt ataggtaaag cacgggcatc tctcaacaac ttctgttttt
    77401 caaggtcaag aagcaagatt cttcctgctt ccaaggatgg accaagctta gcgacgactt
    77461 ctccagtttt tggttgcgct actggcataa ggtgctttta ctcttcttag tcattttgta
    77521 gtcgacgtag tttaaacaag tattagctgc aagcaacaac tttgtaaaac cagacagata
    77581 gattagatgt aaatttcatc caagttccta accagaaaga aagtttagcc atagactttc
    77641 ttacacggga aagacccgat gattttcggc gcgtccatgc tgttctgcag cagctggacg
    77701 ttcaggaaac gcttttcggt accagggcga cgaatgtatt caaagccgta gttgttaccg
    77761 tctttggcag gcataagcac catgtctact ttcaaaccat tggtaccaag ctcggtgatt
    77821 ttttgagagg taactctttc accgttgatt gtcgataact cgccctggtt tgcaaccata
    77881 gtgtagccac cgcatttaac cgtaaagcca tccgcccacg cgctgcacgc agaaaagaca
    77941 gctaacagaa aaataatacc cctcattgct catccccttt gcaaagccga ttgcgtatac
    78001 agatccgccg cgccacgcgc ttcgcacatc atatccatgt accacgcctg gccccttgtg
    78061 tcgccagtgt acataatccc gcgcacaata taaacgccat ccgttgcgat gctggcaggc
    78121 tgcgccgtgg tgccgctgag cgtgatgttt ccgtccgtgt tctggtcagt gatctgccca
    78181 ccagccatag cgatatcgtt gttcgacagc gcggtgcgat acacggaagc ctgatccagt
    78241 tgaatgagtc cgttaacccg gatgttcgga ttaataagcg cgcggacgtt tacgccgttg
    78301 ccgatggtct gctgcggcat accgataagc ccggtagcgc tgttgagcac aatcgctttg
    78361 tgaacatatt cattattcgc caccatctgg cgctgaccgt ccacgaattg ccatgttgcg
    78421 ccacattgcc cggctacgtt atccattaga tgccgcgtca tgccaaagag tacccgcccc
    78481 cgggggaata cagtagcagg catttcaggc gtcaggcctt cggtcgcacc tttggcttcg
    78541 aagtctttca tcagcgcacg gttcacatca gcgaccgtgt aaccggcagc cagcgtctgt
    78601 gaggttatac tggtggcaaa agccagatct gtatcggctg cctgaatcag gacgtaggaa
    78661 tcaaccggac tgtcttttcc tgtgaccgag tagcgaattt caccgctgaa aatcagtccg
    78721 tagttgcgac catcactctg gcccacgtcc gccgcgtcaa cttcccgcac tgtcccgacg
    78781 tcgcttgtcg acacctccgg cgcgataccg tcgtaacccg caatcagacg cactttcgaa
    78841 aactcctgcc cggtaattcg gttcacagta tctgccgaaa ggttataaat tttgatagtc
    78901 cctacccggg acgcgctgct gatgttgaac cagtcgatcg taaaggtgac tttgaaatca
    78961 cttagctcaa ttccctgacc gttcccgtcc acaagctgca gctcgaaatg tctcatccag
    79021 ttctgtgaca tgcttactcc gttgatacca gtaaatgact gcgcccgccc aggtcagttt
    79081 tcgtgggata atcctgtgtg ttgtcatcgc agaccaccaa cagcttaaaa ccaagtccca
    79141 tacaggcgta ctgcgccagc agatcagcgc cagtgacgag aggaataccg gagattaccg
    79201 gctcccctct gtcattcagc aggtccataa tccagtaaag atcgcgccat atgatgctaa
    79261 tccgccaggt gacaccaccc aggacgatgc tgaactgctg gttgtccgct gtcagcggaa
    79321 tttcctgaat tgtcattagc cgccccccag taatgacgcc acgttacccg tgatgctttt
    79381 cagcagtgaa gtatctggag gctttgtggt tttgttgccg ctgttctgta ccgccgacgt
    79441 gctggcccct tccttcatgt tggttttatc cgcgacggta atctgctgtg tccgggagat
    79501 aatgacctcc ctcagggtga ggacggcgga caggacgttt tcggttgtct tgtccgtcgt
    79561 cacttccagc gcccggatca acatgttgct gtacagccgt ttaccggtta ccacatcgaa
    79621 ggggatacgg ctttcctgca gatccagtag ctcctgatac gtctgctgag gactcaggcc
    79681 gagcaggctg gtagccgtca gattactggc aaaatccagc aatgcgccgc caccggcgaa
    79741 accaacctcc atcaccactt ctgacggttt tttataggca tgatcagcga cagcggcccc
    79801 gacttctacc ggatgctcgg ttatttcaag catatctgta tgcttctctg aaataacaac
    79861 actgggaaca atcattccta tttttctgct ctgctgatga aaaagtgtag agagaatatc
    79921 cactaactca ccctcacctg attacttcgc atgacctgag catttgcaga ctgttgccga
    79981 cgtgcaacct cattaccgac agcgtgcgga tctccgccac cgtaaatgtg gtaggtattt
    80041 tgctggttaa cctctgttac tttgccacta attcccgcca cggcagcctt attaatcagc
    80101 tcttgagaat agatatttct tccattttca tgctggataa tgctgctcat caatgctgac
    80161 atggtttgcg gatcgctcat attcagggca gcccggggat ccactcccag tcgttgcgat
    80221 acagccctga tatacgcagt tgtgttgtta ttatcagacg caggcgccca ggtagagata
    80281 attttctcca cgctgtttat tccacgtccg gcgtacaaca ttaactgacg agcaagagcc
    80341 cgtaatccat caaaggcagt ttcgaatctg gcaaatcgcc cgcccgggcg ttcaagagaa
    80401 gcccccgcct gaccagcaaa attaaggttt cccggattgt tattccgttc tcctcgtttc
    80461 gtagcctgtt catgttgttc cggctcatca tcaccaaacc agccgcgtac cgtccggccc
    80521 acactgcggg gatcgaatcc ccagtgctct ttaatccagt cggcagtact gttagcgctg
    80581 tctgtaacca tcggcatcgc tgacggattt tcgctgccct gattaagtat ctgtttgccg
    80641 atgctaacgg catcagccca gcggccatct ttgatagcgt tgagcaggtc ggcgatcatg
    80701 ttcagcattt tgctgaattc gcccatctgg tcgatgaagt tgctgaaatc ccacttcagg
    80761 gaccatgatt tggggtcaat attgagtagt ttcgccagcg ctttcgccag gtcgttaacg
    80821 gtcgttttaa ggtcacgaac catcttcagc gcggcatcga cctccggttt ccacttgccc
    80881 cagtcaatca ggctgtcgcc gccttccttc caggtctgat agtcctccca caggagggca
    80941 atacccgccg ccagcgcggt aatgaggcca atcggcgaca tccagaacgt actgttcaga
    81001 atgcgcagcg caatcgtcag tgcgccaaac agcgagatca actcccgcgt ttgcttatcc
    81061 agcgattgcc accaggtgat gaggcttgat gttccttcaa tcagtctgaa gaacagccgc
    81121 ccgataatat ccccgagcgc cagaatgcct tttatggctt tcgtcagggt ctgctcgata
    81181 cgagggaagt tgtccaggat atggcggcgc agggtgtcca gcgaacccgc aagcccaccc
    81241 gcaagattag agccgatttt gtcacgggcc atgcctgcca tcgcgccaaa ctcacgcagg
    81301 gaggtcataa atttgttgga gcttctggcc gcctcgtcag cattgaagcc gatagctttc
    81361 gccattgcgc tgtactgccc ggagaagcca cccacacccc ggcgcatcgc cataagggta
    81421 ttttcgtcaa tgcccagcat ctgcgcatac tggttagccc ggtaatacgg catgccgctg
    81481 agcttctggc ctacacctgt aaaaatagca gccatgtcac gcatgttacc gctggcatcc
    81541 cgtgtctgta cccccagacg attcagaaat ccctcagccc cggggttatt acggataaaa
    81601 cgggcaaggc tctccagtga cccgcgcgca gcgtctgcac tgccgccaac ctgcgaaacc
    81661 gcatagccaa tagactgaat tccatggacc gtcgcgccgg tgcgctgtga cgcccagtaa
    81721 agattatcca gaccggaggc aatcttagcc gtaaaggcca ccacggacag tgcagctcct
    81781 tcaacggcca accccatttt gatgacattt gcagttgtac cggcgaggac agaaccgaat
    81841 ttttttgctc cagcatcatc cacactgaag ccaagcgaga cgaggaaatc tttaatagtt
    81901 tcagcgttca ttatcctctc tccatttctc aatgcgccgc tggttatccg cttttaccgc
    81961 cagatggtca ttcaagagag caatatcgta caaatcgaca gagccatctt taagtgctgt
    82021 ataaggaatt aacccggcgt caaccggatt gagaaggtag gacagcccgt ccggcaggct
    82081 gttaaacgtc agccctgttg caggctctgc gtcgtgctgg taagggatgt aggcaaaaaa
    82141 tttcccagcg aattggcgac cacccgcgcc accagctgca gcatgaccag caagtcaata
    82201 tcatcaaaca tcagttcgcc ctgggtaaat accggcaccc atccgtccat atgacgccgc
    82261 gataccaccg caagacaggg atgaataatc gcatcggtgt catcttcggt cagggaagac
    82321 aattcctcag cgatacgcgg gagcatggtt tcaaacaccg gttttaactg ctcgaatttc
    82381 acggtgtcga ttttgccgtc ggcaggcaaa cgggagcgaa tgctcccgaa atctgacatc
    82441 attcctgcca gcaccggcag aagtttgcgg gtcactttca gctggtcaaa aacgctgagt
    82501 tttgccgcgc gatatttcac gcctttgatt tcgaattcca tgtattaaaa ctccccgaga
    82561 acctggtcaa tcttgccgca gtcaaacacc cacggcatcg tattaccggt tttagcgttg
    82621 gcattatccg gttgtttctg gaacgcaaca ctacgtgccg tgatgatgtc gccgctgacc
    82681 ttgtttcgga tcacaataac gttattcccc catgtggcag aagactggct ctgtgcgtta
    82741 tacgccagcg acaatttttt atttgtcggt gatgtcttca gaaggttaac ggtaatcgtc
    82801 ccgcttttat ctgcatggag actgtgcatc acttcgccat cagcaccgat ggtcatggtg
    82861 tttttaggac cgcccatcgc aaccacaatc ccctcttcag aacttgcaga accgtacccg
    82921 aggtcaatcg aaccggtcgg cccggtcagc gtcgcagtga catccataaa agaataggta
    82981 gacattcact tccccttagc gaacaacgtt aatctgtacg tcagcgtaat gaaccgcgcc
    83041 tgcaagtttt attgcagcct gaatcaccgg agccttacgg gcttcacgtt ctgattgtgc
    83101 ctgttcatcc agcggctggg cgtatacgta ataacctttg ggcagcgtgt cacctgatga
    83161 caactgacca aggtcgcccc cgttccatac gcccggagca atcagtccat tctgaacggc
    83221 ctgatccagt gatttttcaa cacttgataa cagtcgggta ataccggctt cagtctgggg
    83281 aactttcgtg gtgctggtat aaagcaggtt atagaggttg gtctgcacat aattctgtaa
    83341 ccagtccagg ccgtggcgtt catcaaagaa atcgccgtta gccatcactc cctgctggag
    83401 gatagccgta tcattctggt agtacacgaa tacattgcag ttttttgcat caagtgccga
    83461 tgcctggctg actgtcagtg tttcataccc gacacccggc tcctgcttaa acttgagcgt
    83521 aatcgcggta ttactgccat tgaaattaac cgtgaatgcc cggccaaatg cagataacgc
    83581 agcgtattta ttacccgatg aatattgaat aaaactgcgt gaatatccgg cggttttcag
    83641 ttttgatgcc aaatcatcgc tggatgcagt ctgcaggcat ttctcatcgc ttgtcgtaat
    83701 cgccagaata cggcttacag aagaggattc gatcgccgca gccactttca gccagtctgc
    83761 atccggaata tcttcatcgt ctgcaatccc cagcccatac catgaagtat aatcgagcat
    83821 ggcattcaca gcctgctcca gcgtctcagg cgtggcctgt tcgctgtctc ccttcgtttt
    83881 cacccaacga ccaacaaaaa cctcctgagg tttcggtgat tgtgagaaaa acacctgcgc
    83941 agccttatat tctggtgatt ccacgccaaa atcttttcca atatcttccg cggcagaata
    84001 acggcgaatg cgctcactta ccggaatgat tgtggacggg ccgagaatga gtaatgcacc
    84061 aaaatttcgc cctgatgctg cacgcggcga catgatcaca tcaacattaa caacgtttga
    84121 tacaggcaag ccctgtgcca tagcttaatc tccgaaaaag atgactggtg cttccaccag
    84181 cgatttaata ccgtactcgc gcacaacctt ccggcgcaga cgcaccgtca tatcgtagcg
    84241 gcggacccat tgctgattaa taagttcagg gaagggagtc agacctgtgt aatcgccaag
    84301 agacagcccc agcgcattca gtgctgcgtt gttctgcggt acagatatac cgtcacgaaa
    84361 ccgagacgca tacaccatcc ccgccggacc ataaaacgaa gccatacact caatcgtttc
    84421 atgccgccag agctgagagc catcatcggt ctgtctggtg aatgccggac tgtcatcacc
    84481 tgaccatccg ataaccccaa acgcacacca gttcgtttca gccggtagca gtggcggctg
    84541 ctctttctgc cagcgcggac gaaccatccc ggcagacaga ccggaaacgt tacgcatcca
    84601 ctggcttaac agcctgtcga gcgcttcgtc ataatccgga tcgccactgg ttggtatcag
    84661 ccatccgcgc tctgtgctgg tgttattgct caaccggaat tcccccatca aacggcagca
    84721 actcacaatg cgcctgaacg aatccggcac cataagctgt atacgggtcg acgaaggtca
    84781 cacggtaatc acggccctga tacgtcacga tatcggaatc acggccagtc tgtccctgcg
    84841 tcagtcgctc agtcgtcaca atcagaattg caccactgat tacctgccct gcctgcatac
    84901 ggcggttttc cagagagcga tcaacagtta cgactccggc aaactgcttt ttaacttcac
    84961 tgtcgctgcc gatcccgtcc tcatccaccg tttgcactcg gcgtgttacc cacaaattga
    85021 agtcgcaaaa atcggggtca aaaagcacat ctgttacatc aagagtcggc atctttatct
    85081 ctcacaacat gggtaatggc tctgcgatat tgcccggtgt caattaatgg tttcgccaga
    85141 tcggttcccg gagattcgcc agcagcacgc cgggcaagtt ccagtgttgc ccccttgcgc
    85201 ccccgacgag cccgggcttc aacagtgctg tcagcaagcg gcgtaaagcc ggtaatggtc
    85261 atgtaacgcc tgacgccatt agcggccagc gttccggcac ggttgagtgc gcgttctgct
    85321 cccgcagcat taccatcaag tgcagcctgc gccgcggctt tgagctgcgg caccgtctgc
    85381 tcttctgccg atttaacgcc ggggaccagg tgaggtcgtg gcgggatgtt ctgctctggt
    85441 gagccgtatt cgttgaggta accgatgccc gcattaccaa acggaacatc atcccgcccg
    85501 ctgtcttccg aagggatgcc gaccagcaca tcttttttgg ttaacgacct gagcgcatcc
    85561 agaatggcct tagcgttatc caccctcgtt gttacaccgc ttttgaaact catagctggc
    85621 gaccgcctgc accgaacatc gtgatcaact gataaaattc agcgccatat cgggtgttat
    85681 tccagaagcc tgcatcagga ttcagcgtcg cgctgatgtc ataactgaca cttaccttgt
    85741 caacggactt tgaggactga acaccattgg ttgagccgcc agggccacca gccagcatcg
    85801 ccctgctgtc agccgcccag agcgtcatgt agtgcgcaac gaacaatccg gcaaagtacg
    85861 gaaacaactt tttgccggtg acgttttcgc tcagcagttc atctgccaga ttcagacgaa
    85921 actggatttg cgcttcggga tatttggcag ggtcagcaaa ctgcgggaag tcgcggcgaa
    85981 aatcacttac cgctggcaga ctttgattct ttggcatttt ttacctcgtt acgcgcgtct
    86041 gtggctttgc caacggatac ttccgcgtgc gcacgagtga accagtgcgt ggcaacgtct
    86101 tcctccacag catgacggcc tttaacaaac tcgcgccgtg aaccgtcggg aagcgtgagc
    86161 acaaacgggg tatgtacgtg tattactgca ttattttttg ccatcgggtc atccttaatg
    86221 gccccgccag ggggccatat ggctgttaaa tgccatcaac gtacgaaatg gtttctttgt
    86281 acactggctc gactgcaccc agcttgccgt agtaagtgac gatctgatac agaccgcgat
    86341 actgcaccgg cacgctctga agcggaacca gcgggtagcg gacgtatttt ttatcgttgg
    86401 tgtacgcaac catgcgatcc ttatccccca caccacggcc tttcagccat ttaaccgcgc
    86461 ggatattcag cggaacaccg ttctggtgat agctgatggt gttggtctga aggtacgtca
    86521 acagggactg gttacccgca gatgaaacga tgatgctgga caacagagca aactgctcag
    86581 gcgggatcag caaatcacgc ggaaccacag agtaaccgga agcggcccac gcatcagaca
    86641 gcacctggtt aatgcttgcg cggatttcgt ccggtgttga ggttgcccac gttttggcag
    86701 cgttgttgac aggcacaccg tccagggtaa caagaccttt caggtttaat gcggaatcgc
    86761 caacatacac ctgttcatcg ttatccatct gccatttcag ttgcatcccg tcatacttct
    86821 gcgtatcgat cgggcgtccg acctgctgag cagcctgcaa ttctatgacc gtccagccaa
    86881 gttccatccc ccacaggttc agcgggttac cggatttgcc gatatccacg tttacgccag
    86941 caatagcggt tgagtctttg cctacccagt ttttgccatt cggatttgca ccagtacccg
    87001 cagcggcgaa gctggtattc gtccagctgg aaatgtcatc tgcgatggag acatcttcac
    87061 gcagttggat atcgcgggtc ccggtgtacc ccaccagtgg caggttcagc gtctggtcga
    87121 gccgctccag ctccccgatg agaaaggcac cagagctgtc aacggttgcc tgatcaaaag
    87181 taatcattcg tctgttcctt aaatcttcca ggaaatttct gcattgccgt cagcatcacc
    87241 ggcacctgtg aattcagcgt tggtcagcac cacgtttttg ccactgactg acgtggccat
    87301 gaatccaccc agcggcactt tgatggattc atcagtggag acgacaacgt ataccgggtc
    87361 gccttttttg atggtgctgg catcaaaatc agaaccgaga ttaacggtca cgtagccacg
    87421 cttcattgcg tcgcccggga agttcttgcc actccccacc tggcgaacca tgtccggctg
    87481 cgaagtggtc ggataagggc gcacatagac ccccttcacc ttgtctgcgg tatcaccatc
    87541 tgccagcggc acgaaaaaac cgtcatcatc gtatttacca gccagcccat aggcagcaaa
    87601 ggcgttatcg gatttaagga ccaccggttc gacggttaag tcctgcgggc gagagacagc
    87661 cccggcaatg ccaacaggca tccggtacag aaatacatta ttcatttttt accctttacg
    87721 gtttgcccag aattcagcgt tttgtttgtt cagggaagcg atactggtca tgcctatatt
    87781 tgggcgctgt gcatcgccag tggtggcgcg ggtgtttcgc cctttggcaa tctcagacac
    87841 ggcattaaac gccatgtcga ccgattgttt cggcaatttg cggatatccg catcaccgac
    87901 tatctggcga accagcgttt tgtcagcaga agccagaacc tcgcgtttga acgcggtcgg
    87961 tttcatctta cggctcagat cgatacccgg aacgataact tcggcacgcc aggctgagtc
    88021 accagtaatc gtggtttcct cttcatcgtc ctcgccgtca ccggtcggat tatcgtcagg
    88081 cttattgtcg ttatcgcccg tcgcatttcc ttccagctta gccagcaggg ctttcagtaa
    88141 tgttttgagg tcatcatcac tgtcgccggt tggacctccg cccatctctg gtgctttgtc
    88201 cggtagcggt tgctgcgggg acaggttgat gttgagatta acgccctgcg gcaaatcccc
    88261 ctcatctcct gtaaccgatg cgggagccga ctccaccagt tcgttcatgg tgtcagcgtc
    88321 acccgttttg atggccgtgc gcatgcgggt ccaccagctt ttcttttgat ttgccattgt
    88381 gtctctgtct ccaattgcac aacgatttcc ggctctgcct ttagggacaa gagccacatg
    88441 gtttccggta atatcgacct gcccggcttt acctggctcg gtctgctcat actccgcgtc
    88501 atagccgcac gacacttcgc gcaggccatc ttcgataagc tgaatggcgc tttcgtcttt
    88561 gacgataagg tcagccagca tcaaatcaga ctgctcaccc gtcccgcgcc ggacattctg
    88621 gaggtgcccg acagcaagct ctttccagtt ctcgggattt accagccgca cattcccgtt
    88681 ttcatcttca ggatgcagaa tcgtgatgct catcccttcg aatgaggcaa gcgtggccgg
    88741 atggaatacc tgctcaggag aacgcgtgac gactatttca ccgaacttat cgggtttcag
    88801 ttttggcagg tcatcagcac catagagctg cttacctgtt cgtcctatcg gcacgtcttt
    88861 gcacagcaac gagccgtcag ccagctgata gcgggtttcc cccagccggg tattgaaaaa
    88921 atatttcatg tgttacctgc gattcaggcg ggataagaat gggaggtggg aaaaacgatt
    88981 tctttataac agcgacaatt cgggagctcg ccagcgtgac cggtcatgcc atcaagcgtt
    89041 ggaggtttgc cccattcgac aaatttacct tccatttccc gatgagaatg cctgacgtca
    89101 ccatcttcgg ctgtacgcca gatataacca ttcgaaccaa ttgccagcgc acgcgcctga
    89161 tccagcgcgc cggttgcacg tccaagttca gtacgggcaa tcaggtcagc tctggacttt
    89221 gctatatcac ccgatgcggc tatttcttta gcaaaatgtt ccgctctccc accggtcaca
    89281 acagcttcaa tcgcccgatt ctggatgtcg tacaccctgt cagccgcctc gaggggtagc
    89341 gatttaatgt acttaacctg ttcagcaacg atggatttca tcacctgccc tggcggggca
    89401 ctgtttacca gattgcgtag ctcacggctg atggttttgc tgtgtttacg ccactgctca
    89461 tcattcttgc gcacaatatc ggcggtaaag ttttccgcga cctttgtcgc ccagggtgtg
    89521 atgatttcac tgtagcgttc cagcgcctca ataatttccg tgatactgtc atttgaacca
    89581 tcgtagtgac catttacgat gtccccgacc gcccgcgcta tcctgcgtag gctggttcga
    89641 taccggattt ccgcctgacg gttcctgcgg ttcgtcatca ggttcgccga tgccgggcgg
    89701 cgcttcatct tcggcattct cgatgtcctc gtcggtaatg gatgccccga tgccggttac
    89761 gtcagaattt tctcgcaaat cagtcatagc ggctttcagt gtcatcagac catcacccag
    89821 cgctgtactg attgcgttgg tagtgtttaa cgccaccgtt gagcgatcga catcagacat
    89881 ttgccagagc gggttaaact caaacgtgaa atcgtccggc agcggcttgc caagctccga
    89941 acgatgcatg atgtccagta tccgccgcac cggaagacgt aaacgcctct cctgcaacga
    90001 gcttacccgg tcgtaatagt tggcaaggtc tgcatcgccg gtagaaaatc ccttcgggga
    90061 ctgtccgaac aaccgcacca gtgggatacc aacagcgcca ctaatctgtt ctgcaaacag
    90121 tgaaaggatg tcatccagac cactgaagct gtactgatgg gtttcaaact tatcccgcga
    90181 gtccatgaga gtcatgcctt cattgctctg gaactgtcga atcaggtcaa tattcttcag
    90241 caacgcttca tacgcaggac caccaagtgc gataagctcg cgtagcttct ccacgctgta
    90301 ggtacgcaaa tgcgctttgt agaccagctg cgccgcgccg acagtagcgc tgtcgaacgc
    90361 ggtaagacga tcccagatac gctctacaac cgacattccc cattcgttct cggtcatctt
    90421 ctgctgaaat ggcagcgtga cgccatcaaa gcgaatcagg cgactgtgat gaatgcgcca
    90481 ggcaggaatt cccgttgctg tggtcaccac atcgtaaaac tcaggtttac ccaggtccgg
    90541 ccccatatct ttaatgcggc gggtcagtac cgggtcgatc atccagcggt cgagcgggag
    90601 aatcccctta aacttgccct taccgatggt ttcgggtcgc agcggggtca ttggtgcctg
    90661 cccctcaatc atgatgaaac ccaccgcgcc gccgtagagg cgcgaccatt tcagcacgtc
    90721 attcagcgca tcccagattt gcaactcatc cagttgtgat tcgagaatgc cacaatcttt
    90781 tgcatcaatt tccgaagtga tgcgaatgcc tttgcgggtc atatcatccg ggatagcatc
    90841 gactgcttcg ccgatgatcc aggatgaacg ataggaccat tccaccagca tgcggttacg
    90901 actggtgaaa ttagcccggt aggtggatgc tgagtgctgg ttaggtgtct gcatccctac
    90961 gcgggcaata aaattctcat aaccatcagc tgtagcctgc gcagttcgcc gcagggcttg
    91021 tttgtttcgt gccatcaggc ctgtctccct agcagctccc agatgttcag ggctgaattc
    91081 attggggcat agttgatcat caccgagtcg gcaaggtttg gcgatcgggt tccatcaggc
    91141 tgtttatcaa taacgatttt tcccacacca ttaatggaat aggtcggctg cgaaagctcg
    91201 atgatgagtt tatctttgag tgccatgcta ctgctgattg agatgatttc gtccgggttg
    91261 taagccatac cttcaaccac ggcgcgccag gtattctgaa aaagtttacg taaccgccac
    91321 cagctctggg ctttggcgtt agcgaagaag tccttgttca gacgtgctgc ttgcccgttg
    91381 tccccgcgaa cagcttcatc atccggatca aataccgcgc cactacctcg aaacggtgtg
    91441 gcaagtattg acggtcgacg cgcagcgtta cgcagttcgt tgatagcgcg tgcatcgccg
    91501 cgaacgccag cgcccagccc gtcctcgtca aagcgaaact cttcgaggtt gtcctgttcg
    91561 caaaagccga aaaccttctc gacggactga taaatgtcgc tgcccacacc ggaccattcc
    91621 cgcacatttt ccaggaggaa gccatgacgg gtggaaaagg catttttgtc cctgccttcg
    91681 tcggctacat ccatcgcgcc aagtcgtttg cctgttggct ggatacccag tttgatatgc
    91741 gcatcaacgg cagcctgtac ccattcggat ggaatcagaa cgccttccgc tgatgcgctg
    91801 tagttcagat caagttcctg tgccaccacc accggattat cgattttctc gcattccctg
    91861 cgataccact cttcatcctt gcgaggatca tcccgccagt ggaatgtgaa taccggtatc
    91921 ttcccgccat gacgcttctg agcgaacggg ttagccatgc cgttaactga actcaggtcg
    91981 atacggcaac gcgtcgtttg tgacaacgcc gcatcaatca gcagaggacg ctgaaggaat
    92041 gcagcctcat caaccagata aagcgtggta cggtcaccac gaccaatatt atcgccagcc
    92101 tcgcctttga taacggcacc agtttcagga aactcaacac gcatatatgg cgcgtgcttc
    92161 ttctcactcc acgaaccgcg aaactctaca ggtagcgttt ccacgaactt gcgtgccttc
    92221 cagaacaatg ctttcgggtc accagtgctg tcgacgtatt cctctttacg ggagccgaaa
    92281 ccgataacca tttctttgtt gaagagacaa agcgagcagg ccagtccgat cgcggtccaa
    92341 ctgagcccca tttcacggga tttttcggta ataccattct cccgattgcc ccagcgttcc
    92401 ataatccagt ggatccactc ctcctgctta gggaagagta aaaacggaat ggtcaccggc
    92461 aggccataat caatattacg cgggtccgtt gtcatgcccc agtcgatgat gaactgagcc
    92521 ggattggttc ggtaaaactg cttcaatacg ggcaatattt caggattctg gcgaatgcgc
    92581 tgtaggcgtt ccatccgcca ttcaaaaacc atctggtaat caggatgttt aaaatcgaag
    92641 gggaatggta acggcatact tagcccatca tttttctata cgcctctgca gcctgctccg
    92701 gcgttaagtt ggtaatttct gttctgacgg gtcctccatc agcgccagtc acttcatttt
    92761 tgacgttgtc tttaaacgcc tgaacagaaa catgacgccc aagcaactca aggtttttaa
    92821 ccttatcagg ccacttaatc tttttaagga tcccgaccat ttctctgtca tctccccgcc
    92881 cctcaaacat ttcagcgagg ttaaatccac tcaggtaccg acgccacgat tccggccacg
    92941 cagacagagg cttaatactt aaatcgtcct ccaggatgtc agccacatcg agcctgtcga
    93001 tctcaaccag tcgcatcagc acataattcg catcaatgcc cagttgatca atacgctcct
    93061 gctttagctc gttaatacgg gcgcgtatct caggtttacc gtatagttca gcccccgtaa
    93121 catgtgctcg cctggagacg tagcctgcgc gaatagctgc ttgtgtagca ttcagatcga
    93181 caagaaactc gcgacaaaac acctcgtgtt ttgctttcag cttcttagtc attttatttt
    93241 ccagttatca ggtcattatc gaagcccctc ctggaagagc ttctgtaatg ctattaccgg
    93301 gactgttcta tttgtcggac accagccaac tggttattcg ccttctcgat ggccgccaac
    93361 agtgggttaa tccacagaac agcctggcaa tatgtcaacg ttctggtggt agtggcacga
    93421 tcaccggctg agtcaatgtc cccggaatcg gcgtgcaatg cgctggcgcg taaacggttc
    93481 gcgtagttga gcaacccgac agcaatatca acaggaacag ggaaatcaca gttcttttca
    93541 cggagcagaa cctcacggta tttgatgact gtcttctcgt gaccgatgtc gaccagagaa
    93601 tttaatcggc ttgcgttttc tgctatctgg ttaaaacgat tgaagtttaa tgcctgatta
    93661 gctatcactt tcccttgata ttcagcttca ccttccgctt tatcagcccg caacttttct
    93721 gcctgatact tgctgtggta atggtttgca gaccagacga gcgccccaaa ggcagagaag
    93781 aaaaatgccg cgatgacaat cttataagtc agcttcattt accaccccac cagcatcttt
    93841 aaacctggaa atcaggtcac cgattttatg ttcatactga ccgtaacctg caccaggtaa
    93901 cgacgcccag atattgctgc aacggtcgat tgcctgacga atatcgccac ggtcaatcat
    93961 cggtaaagcg ccacgctctt taatctgctg cagcgccaca gcgtcctgac tttctggcga
    94021 aaaatctttc aggccaagct gcttacagta ggcatcccac cagcgtgaaa gaagctgata
    94081 ccgtcctgcg gctgttgatt taagcttcgg gtttagcgtg acaagtttac gagggtgatc
    94141 ggagtaatca gtgaacaact cacctccgac gataacgtca taaccgtgat tacgtgtcgg
    94201 ttgtcgcccg ttatccgttc cttctgacca tgccaccata tccaggaaag ctttacgttg
    94261 ggaatttagt gtctgcatga attactcctt agagccacca aacttgttac cgattactcg
    94321 cattgcagcc ccacgaatag catccacacc gatcagccca acaccaccac caatggcaac
    94381 agaaagcgat ttaggccatc caacatactc aagagcggat gcaaaggtca gcgtcagagc
    94441 accacagagc aaaatctcaa gcgtttttcg tttccagccg ccaccaccgc caaaataggc
    94501 aatgcgcaag ccagccataa caattgacat aaccactgcg cccaacggcg tatctccacg
    94561 ccaccaactt tgtaagagtt ccagtaagtc aggccaggaa tgaggagcat tgtgcatttt
    94621 catacttccc acctccgcca ttacggggtg ttgttgaaag gcgggccctg cgtatacgcc
    94681 cgtaggattg gggtatgaac cgtccttcgg tgggccctga atacaaaaaa agttcgccat
    94741 agcgaacctt gttaaatttg ttaaaaaagg atgttattta acataatgta cgttatagga
    94801 accacacgat ccccgctcgc aatagatttg cgatgaaagg cctatttaat caacttaagt
    94861 ggtccagaat gacgaaattc gagtgaataa aaggtgcata aaaaagggca aaaaatgcat
    94921 agcgtttttt cgcgacgaaa accctgtttt attaactttt actgagaaca gagcattaaa
    94981 aaagccccaa tgatttggag ctcttcgttg aggtcagaaa ttcaactcat actagcgtag
    95041 cgcagttttt gcgtagcgca ctaacacttt tttcatccct ttgaatttcg acctcaggat
    95101 ccatctgaag gctcacaccc agcatgcaga gacacccctc aatgaatccc tctgctacct
    95161 gcaactgctg gcggataaat ccttcagaac agcgacgccg ccgccctatc tcacgtttgc
    95221 ttaacccgta gacgtagtac agcataatga cgtccagttc ctcaggctta cggacctgct
    95281 gcagccgaca gacgcaagca tcgataatca ggccatcgtc gtcacagcaa ctcagcctgc
    95341 caggcgagct gctgactacc agccctttaa atcccgccgc tattggagcc cagctcactg
    95401 tggtgttgct agcggcagcc cagccacccc agcgctctaa aacctgttga atattacgca
    95461 tagctctgac ctctacgttt tgctacgaaa aaattttctg agaactacgt ctgtttatcc
    95521 gtgggtgtgg ataacgcagt tccgatccgc atattgcatg tatcaacatg caaaaagttg
    95581 ctaattcgga gtggaaccac ctgcccccac ctggaaccac cttttcctaa cctttccctc
    95641 aatcgactta tatatatatg gggtttctag tagaaaggcg gttccagtgg ttccagtagt
    95701 tccgccccgc ttgacacaag gtctgcaagg tggaaccacc ttctctttta ggtggttccg
    95761 cgtggttcca gcggttccca aaccttgcat tttttccccc caattcgcct ttgggcacgc
    95821 ttatacccgc aattttgcaa aacattacta attcgcattt cttcgcgttt tccgatgcgg
    95881 tcaggattta gcccaatcgc atcacgcaaa acatcacttg cgcgtaaaaa ttcgcaattt
    95941 cgcggaagtt cattagtcat caggtcgggc gtgtcgagcc atttctctac cgtttcaagc
    96001 caagcatcct tgatggtgta ctgttcatgg acactcgctg ctagttgttc agcctcacgg
    96061 aactggatcc cccccagacg ctgaaacacc tcacgagctt cagcccaaag taaaaggaga
    96121 tctcttttta tcgctttcac gtcgactttc gacacttcca cggggagcca gcgacggtta
    96181 ccagtcttgt ccgcaaggaa ttcgtcctca ttggtggtac caacgaacac caggcgacga
    96241 ggaaactggg tggcgaactc ccggtatttc gggatccagt tttcgtgagt acgcgtcaca
    96301 aatgctttga ttgactccag ctctttggta ttaagtccac gcagttcacc aatctctgcc
    96361 accagccgac cacgcatttt tcgagcgaga tcatcgtctt tttcggcaaa agagatttca
    96421 gtaaaaaacg caggatcagg gctcagtgcc tccactccgg aagacttacc gcagccctgc
    96481 ggaccaacga gaatcggcac catatccgct ttgatgcctg gctccagtac tcgccccgcc
    96541 aacgcggtcc acatgtacat agacaccgca cgggtataag gcgtgtcggc ggtaccgaag
    96601 tgcgtatggt agaaacattc gatgcgcggc acgccatccc actccagtcc gttcagccag
    96661 gtgatcgctg aatcgaatgg ttgttcatcg gctgcaagta acaccacatc gcgaataagt
    96721 tcacgaccaa caggtttaaa tccccgcttt tccatcgtga tgcgcaggcg cgcataatcc
    96781 gcatcggtga atgcctgcca ttgtccggat cctgcagggg caaacatgat ttcgtcgcgg
    96841 aactggtcaa agcgaatatc gatgtccaca aaatcagggc gtactactgc tttggctgcg
    96901 ttgctgatgg ttgcctcgat acgcccccat ttatcacgct cgaaagcagg cagcggtaaa
    96961 ggttccgcca cttcggtgct ggtcagatct tcgaaatcgt cgttgcggat cccgatggca
    97021 ttaaggaaat ctccgtcatc acgatgcgca caactggcat gcaggcattt aaaatgcccc
    97081 tgctcaaagc ctgcggttcc cgcaggaaaa taaactgtgc ttgttggatc gcctccgcta
    97141 ctgtggccgt cttcaaacgg acagcggata tatcgttcac cgtttgcgcc atccagcagc
    97201 gtccagccat tggcatcaag atattcagct gtatcatccg tcgcgccggg cgtgaatgct
    97261 gaacggtcgc gcatcttcgt gttgcccgct tcggtggtta ccgacacagg gagttgttca
    97321 gccaggcgct gccacagcgt ttcaagctgc tcacctgtaa tagccggagg ttcatccggc
    97381 aaaccaccgt cccattcaat acgcgcgccg ctgctgtgcg taccacaggc aacgaactgc
    97441 tgcccgttcg ccagcaactc gataatgccc atatcccccg ccaggcgatg gatacgctta
    97501 cggaaatcac catcaacggc cagcagatac agacacttat tactgtttgc tcgccagcgt
    97561 cgcggcggcg actcacccag aagttgcaca agtgttttgc gaatatctgc ctgaatgtct
    97621 tcatcttcgc tatcgcagtc cagcgccagc cagccatggc ctgtacgcac gcagatgcca
    97681 taatccggtt cattcgacca gcgggcaaaa tcatgctcag taacaacata cccggtccat
    97741 tgagcaatac cggtgacctg gcggtcccgg ttatagcgac ttggcgtctt acccagcgct
    97801 ttcagtttac tgtcagggga tatagtcgca ccggggttac atacaactgg gagaagatga
    97861 gcagttcgcc ccaataccag gtcgaaatga aaccactcat caggcgtagc tccccatggt
    97921 ttgctatcag acatgggtta agcctttttg tttgactaca tttttttgct ccaaaatctt
    97981 ttcaagttga agagctctta gctctgggat ttcctccccc cattgagata cggctccttt
    98041 ggaaaccttc aatagtctcg ctagtttcgc tttacttcct gcaagctcaa ttgcttctgc
    98101 ttttttcata accgttaccc aggaaatata tggttatatg aggttaagaa aactaaacca
    98161 acaagtcaag aaagcgatac cctgattaag ttaagctaac taaactatat gagggtaaaa
    98221 ccatgattaa tgatcgtatt cgcgaggctc gtcgcaatgt ccacctaacg caggatgcgc
    98281 ttgctaaacg tattggtcta actaaagcca cgatttcaca atgggaatcc ggaaacacca
    98341 cacctaacgg gaaaaatctt ataaatctgg ccgcagcatt gaacgtttct ccagagtggc
    98401 tactttcagg caaaacaagc gataccaaag aaatacattc taatgcacaa gttgaggggg
    98461 ggttttctgt atgggactca tcaacgccat tagaggatga tgaagtggaa ataccgttct
    98521 atcaggaaat tgaactatct gccggtaatg gtacttatgt tgatatagat cgaatgggat
    98581 gtaagttaag attcgcccgt tcaacgttgc gcaaagcagg tgttgacgta aattgtgcgg
    98641 cttgtgtcag catccacggc aacagcatgg aaccagtatt accggatggc gctgtcgtcg
    98701 gcatagatac atcaaaaaca ggtgtaaaag atggtcaaat gtacgctata gaccaagacg
    98761 gattacttcg tgtcaaacta ttgtaccgtc tcccaagcgg actccggata cgctctttca
    98821 atagggatga atacgcagac gaagagtatt ttaatcaaga ggctaataaa atcaggatta
    98881 taggccaagt attttggtac tccgttctgc tctgagcgta aattcactct ataccacatg
    98941 cccgcttcct aagcgggctt ttctttacct atccgaaaaa gttcactttt cttaactaca
    99001 acacttgact ttgtgggttt agaggtctta actatataca taaagttaag atatctatac
    99061 acagatattt aaaccacaag tcgaacggcg cgactctaaa ccatgcgtcg ggaccgtggc
    99121 gggacaggat gtcggcaata cggatcagta agttcccttt ggggtgcggc gaagcactgt
    99181 ctactgatac aagtcgaagc cgccgaacca ccaaagtgaa ctgaatgagg aaacggcgtg
    99241 aaaacgtata aaccgctcaa aggtgaatgc cctgcttgcc aaacacagct tcagacacca
    99301 aagccaaaac gataccaaat caacgaatgt tatgagcact gccctagatg tggtgctttc
    99361 ctgcatacca ttgcaacgca ctggcgtgtc cgcttcaacc ttgtggtacc acgtacgcac
    99421 aataccaact gaatcatcca tgtaattgct gtgtgtagtc tttgcccgcc tcaagtgacg
    99481 ggctttttta tgtctgaaag cgcactcgca acagcgcgct ccccgatatg aaaaaaggaa
    99541 tacaaccaat gaaacctgaa cacatccatc gactgacggg gcgcgatgtt ctccgttatc
    99601 gccgtaaaaa cttcgatttg atgaccggtc tggccgttgt cactgcgctc ggtctgatca
    99661 tcactttcat tctccttgta gcgaggacca cagtatgagt ttagaaacca gtctcgaact
    99721 taataatcaa cttctggcac aacataatgc gctgcttgaa cgtcttatcc gcacaatggc
    99781 atcaggtatt gttatgcatc ctgacacgat ttcacgagtg caggaatgtc gggatacagc
    99841 aactgaaact gaaaatatgt cagcggcaat gacactggat gatctggagt tcagcgacgt
    99901 tatcgcactg gctggtttct acccggtagc cactcctatc acagaagaca tgctgcaacg
    99961 tgctgttgct taccgtgatg ctgaaggcga taaacgagta gttcagattg atgctctcga
   100021 cagcgcattg cagggcgtca aacgagccaa ggcactgcat aaatccgctc ttctggacct
   100081 gtcccgtaac attcttaagt tctgggacga cctgccaacc atcggcgagc gacgtgcttt
   100141 tgccgagcag ctacttgatg cacctgcgga tgggcgtgat gaagttaagc cgaaaaaggc
   100201 cagtaacaaa gatggagaac gcacggggcc gttttacgtc aaaaatgtat ccggcacagc
   100261 agccagtgaa ctccacacct tacgcaagtt gaacgagatg ctgaaaaaag gccatatcga
   100321 gatcaaccgt gttgagtacc ttcagctgca ggaagaattt gcacgcagag acgcagcaaa
   100381 ttccagccag aataatgacg ccaaagatga ccatacaatt gatttcgcgg cactacgcaa
   100441 acaagctgaa gggttgatcc tccagttagc aaaagggggt taccgggcag aagctattgc
   100501 aattctggaa aaacagggag ccaggaaact tggtgaagta acggatgaaa atctcgctga
   100561 agtaatcacc ctggctgaaa aagcactgga gggttaatca tgccagacgt tcacgcacaa
   100621 ctttctccat catcagcgca tcgatggatg cgctgcccag gaagtctggc gctggaggcc
   100681 acacaaccgg ataaagaaac aacttttgca atcgagggca ctgcagcgca cgcgcttgct
   100741 gaaaaagttc tacgaaacag gcaaagccac ccggaacact acgccggatg caatgtttct
   100801 atgtttctcg gctcataccc ccttcgcgaa aatcctgatg atacatctgg cccacaggtg
   100861 gatgatgaaa tggtcgacgc cgttggccgg tatgttgata cggtctggac tcttgcacag
   100921 aataatgaac tattggttga acaacgtgtt gatttctcac atataacggg tgtggaagaa
   100981 tctttcggaa ctgccgacgg cataatcatc gctggtaacg aattacaaat ccacgacctg
   101041 aaatatggca aaggcgtccg cattgatgca gaacaaaatg agcaactaca actgtatgct
   101101 ctgggtgcgc tcgaacaatt cagcatgctg tatgactttg agactgtaag attatttatt
   101161 caccagccaa ggctcaacca cgtttcagaa tggtcgttaa ccgtacagga acttcagtct
   101221 ttcggtgaac gggcacagaa ggccgcaacc agtgcgatcc ttgttctcaa tattgctgaa
   101281 tgcgaaggca ttgagacact accgctggaa aacttcatac ctggagaaaa acagtgccgc
   101341 ttctgtaaag caaaagctat ttgcactgcc cagaaaatgc agcatttaca aacagcggcc
   101401 agcgattttg aagatctgac aaagcctgtc agcgaaataa tcaccaatgc cagcgcacgt
   101461 gtacctctgt taaccattga gcagcttgcg gagatctata gccaggccga ctttattgaa
   101521 tcctggctaa aggcagtacg ggaccgggtt cacaatgaac tcaatgccgg acatccagta
   101581 ccggggttta aactggtaac aggaaaacaa ggtaaccggg cctggagtga tgaagaggca
   101641 gctcgcgcac ttctgaagga ccagttcagg tacaaaactg aagaagtatt cgactttaaa
   101701 cttattagcc ccacaaaagc cgaaaaactt atcaaaaagg ccagtccgcg ccgttggtca
   101761 aaagtcgagg cactgataac acgagctgat ggtaaaccca ccgtcgttcc cgagtcagac
   101821 ccacgccccg cactcaatat caaccctgta aatgatttcg acgacgtatc cgacgatacg
   101881 ctaaccgcag acctcatctg atttaaggaa atacccatga aactgaagtt gaacaatgtt
   101941 cgtctggcct tcccgtctct gtttgaagct aaaactgtaa acggcgaagg cgatccgcgt
   102001 ttctccgcag tatttttaat gtctcccaaa cacccacaac tggaagaaat ccgtaaagct
   102061 atgaagcagg tagcgaagga aaaatggggg gagaagtggg agtccattta taaccagctg
   102121 gagaaaaaac tcaatctgtg cctgcatgat ggtgatgaaa aagcagagta tgaaggcttt
   102181 cccagcaatt tcttcctgaa cgctgctaac aaagcgcgcc cagctgttct tgatcgcgat
   102241 cgttcgccac taattcaggc tgatggacgt ccctatgcag ggtgctatgt aaacgccgtt
   102301 atcgatatct gggcacagga caataatttc ggtaaacgca ttaatgcctc actcggcgga
   102361 gtccagttcc tgcgagacgg cgatgcattc gctggcggcg gagtggcaag cgctgacgat
   102421 ttcgacgata tcagcgaagg tgctgatgct gaagcactaa tttaaagcat caatgtatca
   102481 ggtcgataat atcatttgac ctgatactta cttgcatcta ctttcaataa ctgtaaatta
   102541 tacttattta taacttctcg ccccgggaaa agagtccaga acataaaaaa cgtaaacaaa
   102601 caaaacaaca ttacaacaac gccgagaacc atataaacag tacccttatt tagcaacaaa
   102661 cacagaaata ttaggccata ccccaaataa aaaatagctg caattttaga atgcaagcga
   102721 tatcgt
//
LOCUS       DAZKIV010000002        83839 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZKIV010000002 DAZKIV010000000
VERSION     DAZKIV010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 83839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.002"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            107..241
                     /locus_tag="ACHOT6_000104"
     CDS             107..241
                     /locus_tag="ACHOT6_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945398.1"
                     /translation="MVHYEVVQYLMDCCGITYSQAVQALRSNDWDLWQAEAAICSNKM
                     "
     gene            252..404
                     /gene="ydaF"
                     /locus_tag="ACHOT6_000105"
     CDS             252..404
                     /gene="ydaF"
                     /locus_tag="ACHOT6_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9945399.1"
                     /translation="MQKIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTETGARRW
                     LAKNTS"
     gene            833..1681
                     /locus_tag="ACHOT6_000106"
     CDS             833..1681
                     /locus_tag="ACHOT6_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9945400.1"
                     /translation="MLNVAIENQNGWNYSAPAPYKTGAGRGNPMMITAHNRAQAVFLC
                     VMHSHIQIMVGRAGQPQSWPVSVVAGISTPVRLTTLMVVENLGGELFKLTIEAVIMTT
                     IPTLSHPDVTIENGRAVTTSIAIAEFFGKQHHHVVQKIESLECSEYFLTSNFSRVKFE
                     HRGNSYNAYQITKNGFVFLVMGFTGKKAAAFKEAYIAEFDRMEAELRQNNTTPTNKII
                     PGDGRTLVVRFDKFGNVEFTETVPDDALVCTLDTFRLYLEKQGWTLVNRSAIKNMTVE
                     QLLSIK"
     gene            1728..1949
                     /gene="kilR"
                     /locus_tag="ACHOT6_000107"
     CDS             1728..1949
                     /gene="kilR"
                     /locus_tag="ACHOT6_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="killing protein KilR"
                     /protein_id="HFP9945401.1"
                     /translation="MIAHHFGTDEIPRQCVTPGDYVLHEGRTYIASANNIKKRKLYIR
                     SLTTKTCITDCMIKVFLGRDGLPVKAESW"
     gene            1943..2119
                     /gene="ydaE"
                     /locus_tag="ACHOT6_000108"
     CDS             1943..2119
                     /gene="ydaE"
                     /locus_tag="ACHOT6_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaE family protein"
                     /protein_id="HFP9945402.1"
                     /translation="MVTTKKIKCAYHLCKKDVEESKTIERMLHFMHGTLSKDEPRKYC
                     SEACAEKDQMAHEL"
     gene            2194..2469
                     /locus_tag="ACHOT6_000109"
     CDS             2194..2469
                     /locus_tag="ACHOT6_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945403.1"
                     /translation="MITNYETTVVTTDDIVHEVNLEGKRIGYVIKTKNKETPFTVVDI
                     DGPSGNVKTLDEGVTKMCLVHIGKNLPAEKKAGFLATLIAMKLNGEI"
     gene            2571..5243
                     /gene="recE"
                     /locus_tag="ACHOT6_000110"
     CDS             2571..5243
                     /gene="recE"
                     /locus_tag="ACHOT6_000110"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HFP9945404.1"
                     /translation="MSTKPLFLLRKAKKSSGEPDVVLWASDDFESTCATLDYLIVKSG
                     KKLSNYFKAVATNFPVVNDLPPEGEIDFTWSERYQLSKDSMTWELKPGAAPDDVHHQD
                     NAQETKELAGGLEENAQADAHGDCQDCEVSVATLRFTQRLLHIFTYAAGDRKYLHHAT
                     RKQREHITALEMDQENSYVQNLLLAIRGMAEPTTLDNAALLRLTDAIKAVFSITKKHQ
                     PYEFKNFISAWLDTEHIDRGLLTKEWRKGNRVSRITRTDSGANAGGGNLTDRGEGFVH
                     DQASLARDVATGILARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFRDKFITMPGGL
                     DYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDPEIVDIACGRSSAPMP
                     QRVTEEEKQDDEEKPQPYCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDEKY
                     QKLRAELHETRKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQTRDSVYQN
                     QPETEQNTPETVKTSPDVKQPEPVVQQKPEIVCNACGQTGGDNCPDCGAVMGDATYQE
                     TFNEENLDESQEKEPEEMEGPEHPHNENAGSDLHRDCSDETGEVADPVIAGDIEPGIY
                     YGISNENYHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEP
                     EEFSNRFIVAPEFNRRTNAGKEEEKAFLMECASTGKTVITAEEGRKIELMYQSVMALP
                     LGQWLVESAGHAESSIYWEDPETGILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYY
                     DYRYHVQDAFYSDGYEAQFGVLPTFVFLVASTTVECGRYPIEIFMMGEEAKLAGQQEY
                     HRNLRTLADCLNTDEWPAIKTLSLPRWAKEYAND"
     gene            5236..6045
                     /gene="recT"
                     /locus_tag="ACHOT6_000111"
     CDS             5236..6045
                     /gene="recT"
                     /locus_tag="ACHOT6_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecT"
                     /protein_id="HFP9945405.1"
                     /translation="MTKQPPIAKADLQKTQGNRAPAAVKNNDVISFINQPSMKEQLAA
                     ALPRHMTAERMIRIATTEIRNVPALGNCDTMSFVSAIVQCSQLGLEPGSALGHAYLLP
                     FGNKNEKSGKKNVQLIIGYRGMIDLARRSGQIASLSARVVREGDEFSFEFGLDEKLIH
                     RPGENEDAPVTHVYAVARLKDGGTQFEVMTRKQIELVRSQSKAGNNGPWVTHWEEMAK
                     KTAIRRLFKYLPVSIEIQRAVSMDEKEPLTIDPADSSVLTGEYSVIDNSEE"
     gene            6102..6296
                     /gene="ralR"
                     /locus_tag="ACHOT6_000112"
                     /pseudo
     CDS             6102..6296
                     /gene="ralR"
                     /locus_tag="ACHOT6_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system
                     endodeoxyribonuclease toxin RalR"
     gene            6289..6477
                     /locus_tag="ACHOT6_000113"
     CDS             6289..6477
                     /locus_tag="ACHOT6_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1187 family protein"
                     /protein_id="HFP9945406.1"
                     /translation="MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVS
                     REQKVKLINFNCEKLQSS"
     gene            6577..6792
                     /gene="xisR"
                     /locus_tag="ACHOT6_000114"
     CDS             6577..6792
                     /gene="xisR"
                     /locus_tag="ACHOT6_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase XisR"
                     /protein_id="HFP9945407.1"
                     /translation="MAQVIFNEEWMVEYGLMLRTGLGARQIEAYRQNCWVEGFHFKRV
                     SPLGKPDSKRGIIWYNYPKINQFIKDS"
     gene            6794..8029
                     /gene="intR"
                     /locus_tag="ACHOT6_000115"
     CDS             6794..8029
                     /gene="intR"
                     /locus_tag="ACHOT6_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9945408.1"
                     /translation="MSKLPTGVEIRGRNIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEINSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISYIRYSDILNYRNKLLHGETLYLDNPRSNKKGRTVRT
                     VDNYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGTVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNGKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQVAMLNARLS"
     gene            8081..9016
                     /gene="ttcA"
                     /locus_tag="ACHOT6_000116"
     CDS             8081..9016
                     /gene="ttcA"
                     /locus_tag="ACHOT6_000116"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFP9945409.1"
                     /translation="MQENQKNTKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(9145..10518)
                     /gene="dbpA"
                     /locus_tag="ACHOT6_000117"
     CDS             complement(9145..10518)
                     /gene="dbpA"
                     /locus_tag="ACHOT6_000117"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFP9945410.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQITLNWQTPPVNSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(10548..10721)
                     /gene="ynaL"
                     /locus_tag="ACHOT6_000118"
     CDS             complement(10548..10721)
                     /gene="ynaL"
                     /locus_tag="ACHOT6_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFP9945411.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(10996..11979)
                     /gene="zntB"
                     /locus_tag="ACHOT6_000119"
     CDS             complement(10996..11979)
                     /gene="zntB"
                     /locus_tag="ACHOT6_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFP9945412.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHNDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(11993..12040)
                     /gene="ydaN"
                     /locus_tag="ACHOT6_000120"
     CDS             complement(11993..12040)
                     /gene="ydaN"
                     /locus_tag="ACHOT6_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFP9945413.1"
                     /translation="MLRNRARRLDYKIMR"
     gene            12234..13466
                     /gene="dgcM"
                     /locus_tag="ACHOT6_000121"
     CDS             12234..13466
                     /gene="dgcM"
                     /locus_tag="ACHOT6_000121"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFP9945414.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(13487..14050)
                     /gene="smrA"
                     /locus_tag="ACHOT6_000122"
     CDS             complement(13487..14050)
                     /gene="smrA"
                     /locus_tag="ACHOT6_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFP9945415.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(14380..15288)
                     /gene="abgR"
                     /locus_tag="ACHOT6_000123"
     CDS             complement(14380..15288)
                     /gene="abgR"
                     /locus_tag="ACHOT6_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9945416.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYLGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACIRLVAKSDFLSILPEEMGCDPLHGLGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            15464..16774
                     /gene="abgA"
                     /locus_tag="ACHOT6_000124"
     CDS             15464..16774
                     /gene="abgA"
                     /locus_tag="ACHOT6_000124"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFP9945417.1"
                     /translation="MESLNQFVNSLAPKLSYWRRDFHHYAESGWVEFRTATLVAEELH
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVFAIAVETLARTALNFPWTRGI"
     gene            16774..18219
                     /gene="abgB"
                     /locus_tag="ACHOT6_000125"
     CDS             16774..18219
                     /gene="abgB"
                     /locus_tag="ACHOT6_000125"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFP9945418.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYQALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            18256..19782
                     /gene="abgT"
                     /locus_tag="ACHOT6_000126"
     CDS             18256..19782
                     /gene="abgT"
                     /locus_tag="ACHOT6_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFP9945419.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVLSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            19793..20308
                     /gene="ogt"
                     /locus_tag="ACHOT6_000127"
     CDS             19793..20308
                     /gene="ogt"
                     /locus_tag="ACHOT6_000127"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFP9945420.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTISC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISVVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            20503..21255
                     /gene="fnr"
                     /locus_tag="ACHOT6_000128"
     CDS             20503..21255
                     /gene="fnr"
                     /locus_tag="ACHOT6_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFP9945421.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            21407..22357
                     /gene="uspE"
                     /locus_tag="ACHOT6_000129"
     CDS             21407..22357
                     /gene="uspE"
                     /locus_tag="ACHOT6_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFP9945422.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(22407..22664)
                     /gene="ynaJ"
                     /locus_tag="ACHOT6_000130"
     CDS             complement(22407..22664)
                     /gene="ynaJ"
                     /locus_tag="ACHOT6_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFP9945423.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            22908..23939
                     /gene="ynaI"
                     /locus_tag="ACHOT6_000131"
     CDS             22908..23939
                     /gene="ynaI"
                     /locus_tag="ACHOT6_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFP9945424.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
     gene            complement(23990..25603)
                     /gene="mppA"
                     /locus_tag="ACHOT6_000132"
     CDS             complement(23990..25603)
                     /gene="mppA"
                     /locus_tag="ACHOT6_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFP9945425.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKSGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(25940..26839)
                     /gene="pgrR"
                     /locus_tag="ACHOT6_000133"
     CDS             complement(25940..26839)
                     /gene="pgrR"
                     /locus_tag="ACHOT6_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFP9945426.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            26977..27897
                     /locus_tag="ACHOT6_000134"
     CDS             26977..27897
                     /locus_tag="ACHOT6_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9945427.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            27897..28829
                     /locus_tag="ACHOT6_000135"
     CDS             27897..28829
                     /locus_tag="ACHOT6_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9945428.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            28847..29485
                     /locus_tag="ACHOT6_000136"
     CDS             28847..29485
                     /locus_tag="ACHOT6_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9945429.1"
                     /translation="MKNVLILGAGGQIAHHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKCTIVSRKSVAALITDIIDKPEKHIGENIGINQSVTDGDKPLFM"
     gene            29795..30523
                     /gene="mpaA"
                     /locus_tag="ACHOT6_000137"
     CDS             29795..30523
                     /gene="mpaA"
                     /locus_tag="ACHOT6_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFP9945430.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(30498..31463)
                     /gene="ycjG"
                     /locus_tag="ACHOT6_000138"
     CDS             complement(30498..31463)
                     /gene="ycjG"
                     /locus_tag="ACHOT6_000138"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFP9945431.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            31582..32088
                     /gene="tpx"
                     /locus_tag="ACHOT6_000139"
     CDS             31582..32088
                     /gene="tpx"
                     /locus_tag="ACHOT6_000139"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFP9945432.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(32132..33673)
                     /gene="tyrR"
                     /locus_tag="ACHOT6_000140"
     CDS             complement(32132..33673)
                     /gene="tyrR"
                     /locus_tag="ACHOT6_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFP9945433.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(33821..34882)
                     /gene="ycjF"
                     /locus_tag="ACHOT6_000141"
     CDS             complement(33821..34882)
                     /gene="ycjF"
                     /locus_tag="ACHOT6_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFP9945434.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(34879..36276)
                     /gene="ycjX"
                     /locus_tag="ACHOT6_000142"
     CDS             complement(34879..36276)
                     /gene="ycjX"
                     /locus_tag="ACHOT6_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFP9945435.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            36431..37429
                     /gene="ycjW"
                     /locus_tag="ACHOT6_000143"
     CDS             36431..37429
                     /gene="ycjW"
                     /locus_tag="ACHOT6_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9945436.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(37540..38445)
                     /gene="ompG"
                     /locus_tag="ACHOT6_000144"
     CDS             complement(37540..38445)
                     /gene="ompG"
                     /locus_tag="ACHOT6_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFP9945437.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(38490..39572)
                     /locus_tag="ACHOT6_000145"
     CDS             complement(38490..39572)
                     /locus_tag="ACHOT6_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9945438.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(39586..40245)
                     /gene="pgmB"
                     /locus_tag="ACHOT6_000146"
     CDS             complement(39586..40245)
                     /gene="pgmB"
                     /locus_tag="ACHOT6_000146"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFP9945439.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(40242..42509)
                     /gene="ycjT"
                     /locus_tag="ACHOT6_000147"
     CDS             complement(40242..42509)
                     /gene="ycjT"
                     /locus_tag="ACHOT6_000147"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFP9945440.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLMETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LAQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVHDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDY
                     ILPFNRTATTHQEDE"
     gene            complement(42506..43561)
                     /gene="ycjS"
                     /locus_tag="ACHOT6_000148"
     CDS             complement(42506..43561)
                     /gene="ycjS"
                     /locus_tag="ACHOT6_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9945441.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(43571..44359)
                     /gene="ycjR"
                     /locus_tag="ACHOT6_000149"
     CDS             complement(43571..44359)
                     /gene="ycjR"
                     /locus_tag="ACHOT6_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9945442.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(44377..45429)
                     /gene="ycjQ"
                     /locus_tag="ACHOT6_000150"
     CDS             complement(44377..45429)
                     /gene="ycjQ"
                     /locus_tag="ACHOT6_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFP9945443.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILSNEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(45460..46302)
                     /gene="ycjP"
                     /locus_tag="ACHOT6_000151"
     CDS             complement(45460..46302)
                     /gene="ycjP"
                     /locus_tag="ACHOT6_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9945444.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(46289..47170)
                     /gene="ycjO"
                     /locus_tag="ACHOT6_000152"
     CDS             complement(46289..47170)
                     /gene="ycjO"
                     /locus_tag="ACHOT6_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFP9945445.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(47191..48483)
                     /gene="ycjN"
                     /locus_tag="ACHOT6_000153"
     CDS             complement(47191..48483)
                     /gene="ycjN"
                     /locus_tag="ACHOT6_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9945446.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYTKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPSTLQASQKKLDELIEQH"
     gene            complement(48497..50176)
                     /gene="ycjM"
                     /locus_tag="ACHOT6_000154"
     CDS             complement(48497..50176)
                     /gene="ycjM"
                     /locus_tag="ACHOT6_000154"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFP9945447.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQQPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(50388..50702)
                     /gene="pspE"
                     /locus_tag="ACHOT6_000155"
     CDS             complement(50388..50702)
                     /gene="pspE"
                     /locus_tag="ACHOT6_000155"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFP9945448.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(50777..50998)
                     /gene="pspD"
                     /locus_tag="ACHOT6_000156"
     CDS             complement(50777..50998)
                     /gene="pspD"
                     /locus_tag="ACHOT6_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFP9945449.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(51007..51366)
                     /gene="pspC"
                     /locus_tag="ACHOT6_000157"
     CDS             complement(51007..51366)
                     /gene="pspC"
                     /locus_tag="ACHOT6_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFP9945450.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(51366..51590)
                     /gene="pspB"
                     /locus_tag="ACHOT6_000158"
     CDS             complement(51366..51590)
                     /gene="pspB"
                     /locus_tag="ACHOT6_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFP9945451.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(51641..52312)
                     /gene="pspA"
                     /locus_tag="ACHOT6_000159"
     CDS             complement(51641..52312)
                     /gene="pspA"
                     /locus_tag="ACHOT6_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFP9945452.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQQ"
     gene            52479..53456
                     /gene="pspF"
                     /locus_tag="ACHOT6_000160"
     CDS             52479..53456
                     /gene="pspF"
                     /locus_tag="ACHOT6_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFP9945453.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(53576..54841)
                     /gene="puuE"
                     /locus_tag="ACHOT6_000161"
     CDS             complement(53576..54841)
                     /gene="puuE"
                     /locus_tag="ACHOT6_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFP9945454.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPELVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYVDKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(54879..56159)
                     /gene="puuB"
                     /locus_tag="ACHOT6_000162"
     CDS             complement(54879..56159)
                     /gene="puuB"
                     /locus_tag="ACHOT6_000162"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFP9945455.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(56161..57648)
                     /gene="puuC"
                     /locus_tag="ACHOT6_000163"
     CDS             complement(56161..57648)
                     /gene="puuC"
                     /locus_tag="ACHOT6_000163"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFP9945456.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(57923..58480)
                     /gene="puuR"
                     /locus_tag="ACHOT6_000164"
     CDS             complement(57923..58480)
                     /gene="puuR"
                     /locus_tag="ACHOT6_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFP9945457.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(58507..59271)
                     /gene="puuD"
                     /locus_tag="ACHOT6_000165"
     CDS             complement(58507..59271)
                     /gene="puuD"
                     /locus_tag="ACHOT6_000165"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFP9945458.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            59483..60901
                     /gene="puuA"
                     /locus_tag="ACHOT6_000166"
     CDS             59483..60901
                     /gene="puuA"
                     /locus_tag="ACHOT6_000166"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFP9945459.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            61050..61214
                     /locus_tag="ACHOT6_000167"
     CDS             61050..61214
                     /locus_tag="ACHOT6_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945460.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            61204..62589
                     /gene="puuP"
                     /locus_tag="ACHOT6_000168"
     CDS             61204..62589
                     /gene="puuP"
                     /locus_tag="ACHOT6_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFP9945461.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            62723..62968
                     /gene="ymjA"
                     /locus_tag="ACHOT6_000169"
     CDS             62723..62968
                     /gene="ymjA"
                     /locus_tag="ACHOT6_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFP9945462.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            63281..64924
                     /gene="sapA"
                     /locus_tag="ACHOT6_000170"
     CDS             63281..64924
                     /gene="sapA"
                     /locus_tag="ACHOT6_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFP9945463.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYDPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            64921..65886
                     /gene="sapB"
                     /locus_tag="ACHOT6_000171"
     CDS             64921..65886
                     /gene="sapB"
                     /locus_tag="ACHOT6_000171"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFP9945464.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            65873..66763
                     /gene="sapC"
                     /locus_tag="ACHOT6_000172"
     CDS             65873..66763
                     /gene="sapC"
                     /locus_tag="ACHOT6_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFP9945465.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            66763..67755
                     /gene="sapD"
                     /locus_tag="ACHOT6_000173"
     CDS             66763..67755
                     /gene="sapD"
                     /locus_tag="ACHOT6_000173"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFP9945466.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            67757..68563
                     /gene="sapF"
                     /locus_tag="ACHOT6_000174"
     CDS             67757..68563
                     /gene="sapF"
                     /locus_tag="ACHOT6_000174"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFP9945467.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            68631..68984
                     /gene="ycjD"
                     /locus_tag="ACHOT6_000175"
     CDS             68631..68984
                     /gene="ycjD"
                     /locus_tag="ACHOT6_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFP9945468.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            69352..70140
                     /gene="fabI"
                     /locus_tag="ACHOT6_000176"
     CDS             69352..70140
                     /gene="fabI"
                     /locus_tag="ACHOT6_000176"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFP9945469.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            70285..71412
                     /gene="yciW"
                     /locus_tag="ACHOT6_000177"
                     /pseudo
     CDS             70285..71412
                     /gene="yciW"
                     /locus_tag="ACHOT6_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            71480..73414
                     /gene="rnb"
                     /locus_tag="ACHOT6_000178"
     CDS             71480..73414
                     /gene="rnb"
                     /locus_tag="ACHOT6_000178"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFP9945470.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(73492..73596)
                     /locus_tag="ACHOT6_000179"
     CDS             complement(73492..73596)
                     /locus_tag="ACHOT6_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945471.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQMKVS"
     gene            73650..75635
                     /gene="pdeR"
                     /locus_tag="ACHOT6_000180"
     CDS             73650..75635
                     /gene="pdeR"
                     /locus_tag="ACHOT6_000180"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9945472.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGISLSPEHGSDSATIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLALFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(75617..75745)
                     /gene="ymiD"
                     /locus_tag="ACHOT6_000181"
     CDS             complement(75617..75745)
                     /gene="ymiD"
                     /locus_tag="ACHOT6_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFP9945473.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            75782..75955
                     /gene="yciZ"
                     /locus_tag="ACHOT6_000182"
     CDS             75782..75955
                     /gene="yciZ"
                     /locus_tag="ACHOT6_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFP9945474.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            76045..76794
                     /gene="yciT"
                     /locus_tag="ACHOT6_000183"
     CDS             76045..76794
                     /gene="yciT"
                     /locus_tag="ACHOT6_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFP9945475.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            77063..77281
                     /gene="osmB"
                     /locus_tag="ACHOT6_000184"
     CDS             77063..77281
                     /gene="osmB"
                     /locus_tag="ACHOT6_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFP9945476.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(77407..77733)
                     /gene="yciH"
                     /locus_tag="ACHOT6_000185"
     CDS             complement(77407..77733)
                     /gene="yciH"
                     /locus_tag="ACHOT6_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFP9945477.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(77733..78470)
                     /gene="pyrF"
                     /locus_tag="ACHOT6_000186"
     CDS             complement(77733..78470)
                     /gene="pyrF"
                     /locus_tag="ACHOT6_000186"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFP9945478.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDAALAFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDMKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGNDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(78663..79832)
                     /gene="lapB"
                     /locus_tag="ACHOT6_000187"
     CDS             complement(78663..79832)
                     /gene="lapB"
                     /locus_tag="ACHOT6_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFP9945479.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTADTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(79839..80147)
                     /gene="lapA"
                     /locus_tag="ACHOT6_000188"
     CDS             complement(79839..80147)
                     /gene="lapA"
                     /locus_tag="ACHOT6_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFP9945480.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(80296..81060)
                     /gene="pgpB"
                     /locus_tag="ACHOT6_000189"
     CDS             complement(80296..81060)
                     /gene="pgpB"
                     /locus_tag="ACHOT6_000189"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFP9945481.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            81230..81820
                     /gene="ribA"
                     /locus_tag="ACHOT6_000190"
     CDS             81230..81820
                     /gene="ribA"
                     /locus_tag="ACHOT6_000190"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFP9945482.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(81884..>83839)
                     /gene="acnA"
                     /locus_tag="ACHOT6_000191"
     CDS             complement(81884..>83839)
                     /gene="acnA"
                     /locus_tag="ACHOT6_000191"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFP9945483.1"
                     /translation="PVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVGKF
                     VEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELVEK
                     YAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNELE
                     VNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVILG
                     LKRQPWVKASLAPGSKVVSDYLAKAKLTSFLDELGFNLVGYGCTTCIGNSGPLPDPIE
                     TAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLASEPIG
                     HDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDTYG
                     WQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSPAG
                     RYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSDVV
                     SIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRSNL
                     IGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVPCR
                     CRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        20937 a        21520 c        20632 g        20750 t
ORIGIN      
        1 gcatatttgt gttgatcatt aatatgaaca taactaatat tattacagaa gcagcacggc
       61 gctgtaggtt ttagttccgc cacccggcgt taaagggaga gataaaatgg tgcattacga
      121 agtagttcag tatttgatgg attgttgcgg tatcacttac agccaagctg tacaggctct
      181 acgcagcaac gactgggatc tctggcaggc agaagccgct atatgcagca acaagatgtg
      241 agattcgcaa aatgcaaaaa atcgacctcg gcaacaacga atccctagtg tgtggcgtgt
      301 tccccaacca ggatggaacg ttcaccgcca tgacgtatac caaaagcaaa acatttaaaa
      361 ctgaaacagg cgcacgtcgc tggcttgcca aaaataccag ctaatccatt tattggatta
      421 attcaatatt ctcgctgtag aggtatagcc gaggccacca aagcccggag gtggtgaaat
      481 aaaaccgggc ataacacgaa ggcgcatttc cggtatccat aaagagtcgg tcttgtctgt
      541 caaatttaaa tggtgggagt gcgcctccgg ttgtgaataa caacactgct gtgtgtagtc
      601 ttggcggcat cagttttttc ttgaagttcg actgatgtcc gcccttttta aagtgaattt
      661 tgtgatgcgg tgaatgcggc taagcgcacg cggaacagtt aaaaagctat tgtccttcgt
      721 agcagagtta tgggtgaaat atccggcgtt aattgttaac tggttaacgt cacctggagg
      781 caccaggcac cgcatcaaca aagttcactt cggtgatgaa aggtgagaga aaatgttgaa
      841 tgtagctatt gaaaaccaga acgggtggaa ttatagtgca cctgcacctt ataaaacggg
      901 tgccgggcgt ggaaacccga tgatgattac cgcgcataac cgcgctcagg cggttttttt
      961 atgcgtaatg cacagccaca ttcagattat ggtggggcgt gcagggcagc cgcaaagctg
     1021 gccggtttcg gtagtcgccg gtatttccac ccctgtacgt ctcaccaccc ttatggtcgt
     1081 ggaaaacctt ggtggtgagt tatttaaact gactatcgag gctgtcatta tgactactat
     1141 ccctaccctt tctcaccctg acgtaaccat cgaaaacggt cgcgctgtaa ctacatccat
     1201 tgcgatcgca gagttttttg gcaagcaaca tcatcatgtt gtccaaaaaa tagaatccct
     1261 cgaatgctct gaatattttt taaccagcaa cttttcgcgg gttaaattcg aacatcgcgg
     1321 caattcctat aatgcctacc agatcaccaa aaacggcttc gttttcctgg tgatgggctt
     1381 cactggcaaa aaagccgctg cattcaaaga ggcctacatc gctgagttcg accgcatgga
     1441 agcagaacta cgccagaata ataccactcc cacaaacaaa atcattccgg gcgatgggcg
     1501 cactctggtt gttcgcttcg acaaattcgg caacgtcgaa ttcactgaaa ccgttcctga
     1561 tgacgctctc gtctgtaccc tagacacttt ccgcctttat ctggagaaac agggctggac
     1621 tcttgtaaac cgaagcgcaa ttaaaaacat gaccgtcgag cagttgctga gtattaaata
     1681 gttttctgga attttcttaa tacgacaaat ttattaagga gataattatg attgctcatc
     1741 acttcggaac cgatgaaata ccacgccagt gcgtgactcc tggcgattat gttcttcatg
     1801 aaggccggac atatattgcc tcggcaaaca atattaaaaa gcgaaaactt tatattcgta
     1861 gcctaactac aaaaacatgc attactgact gcatgattaa agtcttcctc ggtcgtgatg
     1921 gtttacctgt aaaggcggag tcatggtgac gactaagaaa ataaaatgtg cttaccacct
     1981 ttgcaaaaaa gacgttgaag aaagcaaaac tatagaaaga atgcttcact tcatgcacgg
     2041 gactttatca aaagacgaac cgagaaaata ttgcagtgaa gcttgtgccg aaaaagacca
     2101 gatggcacac gaactttaat taattgacta ttcgaaactg aatttatgcc agaaatggca
     2161 gggattcgct caaccttaat tcaggagaaa aacatgatta ccaattatga aaccactgtt
     2221 gtaactaccg atgacattgt tcacgaggtg aatctggaag gaaagcgcat tggctacgta
     2281 attaaaacaa aaaataaaga aaccccattc actgtggttg atatcgatgg tccatcaggc
     2341 aacgtaaaaa cacttgatga aggtgtcaca aaaatgtgcc tggttcacat cggaaagaat
     2401 ctgcccgcag aaaaaaaagc cggatttctg gcaactctga ttgcaatgaa attaaacggt
     2461 gaaatctgaa agaaatagcc tgcgtatggc gcaggctatg aacagtgtgt atccggcaag
     2521 atcattcact gaacaaaacg aattttaatc tgagttgagg ttaaaaaaca atgagcacaa
     2581 aaccactctt ccttttacgg aaagcgaaaa aatcatccgg tgagcctgac gtcgtcctgt
     2641 gggcaagcga cgattttgaa tcaacctgtg ccactctgga ctacctgatc gttaagtcag
     2701 gtaaaaaact gagcaactat tttaaagctg ttgccacgaa ttttcctgtc gttaatgacc
     2761 tgccccctga aggtgagatc gattttacct ggagtgaacg ctatcaactc agcaaagact
     2821 ccatgacctg ggaactaaaa ccgggagcag caccagacga cgttcaccac caggataatg
     2881 ctcaggaaac caaagaactg gcgggaggcc tggaagaaaa cgcgcaggca gacgcccacg
     2941 gggattgcca ggattgcgaa gtctctgtag ccactttgcg gttcacacag cgtcttctgc
     3001 acatttttac gtatgcggcc ggggatcgga aatacctgca tcatgccacc cgtaaacaac
     3061 gcgaacacat taccgctctt gagatggatc aggaaaacag ctatgtccag aatctgctgt
     3121 tggccatacg cggcatggca gaaccgacaa ctctggataa tgccgccctg ctccgcctga
     3181 ctgatgcaat taaggcagtt ttctctatca cgaaaaaaca tcagccctat gaatttaaga
     3241 atttcatttc agcctggctg gacaccgaac acattgatcg cggtcttcta acaaaagaat
     3301 ggcgaaaagg gaatcgtgtt tcacgcatca ctcgcacaga ttccggtgct aatgcaggcg
     3361 gcgggaacct taccgatcgc ggcgaaggtt tcgttcatga tcaggcgtcg ctggcccgcg
     3421 acgtagccac tggcattctg gcccgttcaa tggacgtgga catctataac cttcatccgg
     3481 cacacgctaa acgcattgag gaaattatcg ctgaaaataa accgcccttt tctgttttcc
     3541 gcgacaaatt catcaccatg cctggcgggc tggattattc ccgcgccatc gtggttgcgt
     3601 ccgtgaaaga agcaccaatt gggatcgagg tcatccccgc gcacgtcact gaatatctga
     3661 acaaagtact gactgaaact gatcatgcca accctgatcc ggaaatcgtg gatattgcct
     3721 gcggtcgttc ctctgccccg atgccgcagc gtgtaacaga agaagaaaaa caggatgatg
     3781 aagaaaaacc gcaaccatat tgcgcaatgg cagatgaaca ggcaacggct gaaacagtgg
     3841 aaccggatgc aactgaacat catcaggaca cgcagccgct ggatgctcag tcacaggtaa
     3901 attctgttga tgaaaaatat cagaaactgc gggcagaact ccatgaaaca cggaaaaaca
     3961 ttccgcccaa aaatcctgtc gatgcagaca aattactggc tgcttcgcgt ggtgagttcg
     4021 ttgaagggat tagcgacccg aacgatccga aatgggtaaa ggggatccag actcgcgact
     4081 ctgtgtacca gaaccagcca gaaacggaac aaaacacgcc agaaactgta aaaaccagcc
     4141 cggatgtgaa acaacctgaa ccagtagtgc aacagaaacc ggaaatagtc tgcaatgcct
     4201 gcggtcagac tggcggggat aactgccctg actgtggtgc ggtgatgggc gacgcaacgt
     4261 atcaggaaac ctttaatgaa gaaaatctgg atgaatctca ggaaaaagag ccggaggaaa
     4321 tggaaggccc tgaacatccg cacaatgaga atgctggcag cgatctgcat cgcgattgca
     4381 gtgatgaaac tggcgaagtc gcagatcccg taatcgcagg agacatagag cctggtattt
     4441 attacggaat ttcgaatgag aattaccacg cgggtcccgg tgtcagtaag tctcagctcg
     4501 acgacattgc tgatactccg gcgctgtatt tgtggcgtaa aaatgcccct gtggacacca
     4561 caaagacaaa aacgctcgat ttaggaaccg ctttccactg ccgtgtactt gaaccggaag
     4621 aattcagtaa ccgctttatc gtagcacctg aatttaaccg ccgtacaaac gccggaaaag
     4681 aagaagagaa agcgtttctg atggaatgcg caagcacagg aaaaacggtt atcactgccg
     4741 aagaaggccg gaaaattgaa ctcatgtatc agagcgttat ggctttgccg ctgggtcaat
     4801 ggcttgtgga aagcgccgga cacgctgaat catcaattta ctgggaagat ccggaaacag
     4861 gaattttgtg tcggtgccgt ccggacaaaa ttattcctga atttcactgg atcatggacg
     4921 tgaaaaccac agcggatatt caacgattca aaacggctta ttacgactac cgctatcacg
     4981 ttcaggatgc attctacagt gacggttatg aagcacagtt tggtgtgctg ccaactttcg
     5041 tttttctggt tgccagcaca actgttgaat gcggacgtta cccgattgag attttcatga
     5101 tgggcgaaga agcaaaactg gcaggccagc aggaatatca ccgcaatctg aggaccctgg
     5161 ctgactgcct caataccgat gaatggccag ctattaagac gttatcactg ccccgctggg
     5221 ctaaggaata tgcaaatgac taagcaacca ccaatcgcaa aagccgatct gcaaaaaact
     5281 cagggaaacc gtgcaccagc agcagttaaa aataacgacg tgattagttt tattaaccag
     5341 ccatcaatga aagagcaact ggcagcagct cttccacgcc atatgacggc tgaacgtatg
     5401 atccgtatcg ccaccacaga aattcgtaac gttccagcgt taggaaactg tgacactatg
     5461 agttttgtca gtgcaatcgt acagtgttca cagctcggcc ttgagccagg tagcgccctt
     5521 ggccacgcat atttactgcc ttttggtaat aaaaacgaaa agagcggtaa aaagaacgtt
     5581 cagctaatca ttggctatcg cggcatgatt gatctggctc gccgttctgg tcaaatcgcc
     5641 agcctgtcag cccgtgttgt ccgtgaaggt gacgagttta gcttcgaatt tggccttgat
     5701 gaaaagttaa tacaccgccc gggagaaaac gaagatgcac cagtgaccca cgtctatgct
     5761 gtcgcaagac tgaaagacgg agggactcag tttgaagtta tgacgcgcaa acagattgaa
     5821 ctggtgcgca gccagagtaa ggcaggtaat aacgggccgt gggtaactca ctgggaagaa
     5881 atggcaaaaa aaacggctat tcgtcgcctg tttaaatacc tgcctgtctc aattgaaatc
     5941 cagcgtgcag tatcaatgga tgaaaaggaa ccactgacaa tcgatccggc agactcctct
     6001 gtattaaccg gggaatacag tgtaatcgat aattcagaag aataattcag ccaggcggtg
     6061 taatgcaccg ccaacgtgag atagttttta tgacaaaaac tttgagatat gacgatgtta
     6121 aaccatgtcc gttttgtggt tgtccatcag taacggtgaa agacatttca tgatattacc
     6181 gggcaaaatg caacggatgc gaatcccgaa ctggctatgg tggaagtgaa aaagaagcgc
     6241 tcgaaagatg ggataaacga accactgaaa atattaatgg aggcgttcat gtataaaatt
     6301 accgctacaa ttgaaaagga aggtggcact cctactaact ggacaagata ttcaaaatct
     6361 aaactaacga aatcagaatg cgaaaaaatg ctctcaggta aaaaagaagc aggcgtttcc
     6421 agagagcaga aagtaaaact gataaatttt aattgcgaga aacttcagtc ctcgtgaatt
     6481 gcattgtatt caaattaaaa cttcatagct gattattaat aatcaacatc aggcgtcaat
     6541 ttcagtctaa cattggcgcc tgccagaggt gatgcgatgg cacaagtaat ctttaatgaa
     6601 gagtggatgg ttgaatacgg cctgatgctt cgcactggtc tgggggccag acaaattgaa
     6661 gcataccgcc agaactgttg ggtggagggc ttccacttca aacgagtatc tcctttaggt
     6721 aagccagaca gcaaacgagg gattatctgg tacaactatc caaagataaa tcagtttatc
     6781 aaagactcat gatatgtcta aattaccaac aggtgtcgag attagaggta gaaacattcg
     6841 catctggttc atgtttcgag gaaaacgatg tcgggaaaca ttaaaaggct gggagattac
     6901 aaacagtaat attaaaaagg ccggaaattt aagagcgctg atagttcatg aaataaactc
     6961 cggtgaattt gagtatttaa gacgttttcc ccagtccagc actggggcaa aaatggtgac
     7021 aacgagagtc ataaaaacgt tcggggagct ttgtgatatc tggacaaaaa ttaaagagac
     7081 agagttaaca acaaacacaa tgaagaaaac gaaatcacaa ttaaaaacac tcagaataat
     7141 aatttgtgaa agtaccccga tatcatatat tcgttatagc gatatcttaa actaccggaa
     7201 taaactgctg catggagaaa cgctttacct ggataatcca agatccaaca aaaaaggaag
     7261 aaccgtgcgc acagttgata actatatcgc cctgctctgt tcgctgttac gttttgcgta
     7321 tcagtcggga tttatatcaa ccaaaccatt tgaaggagta aaaaaattac agcgaaacag
     7381 aataaagcct gacccgttat ctaaaacaga attcaatgca ttaatggaaa gtgaaaaagg
     7441 acagagccaa aacttgtgga aatttgccgt ttactccggg cttcgtcacg gggaactggc
     7501 agctctggcg tgggaggatg tggatttcga gaagggaact gtgaatgtca gaagaaacct
     7561 gacgatactg gatatgttcg gtcccccaaa aacaaatgcc gggatccgga cagtaacatt
     7621 actgcagcct gctcttgaag cactgaagga gcaatacaaa ctgaccgggc atcatcgcaa
     7681 aagcgaaatc accttttatc atcgggagta cggcagaacc gaaaagcaaa aactgcattt
     7741 tgttttcatg cccagggtgt gtaacggaaa acagaaacct tattactcgg taagcagttt
     7801 gggggcaagg tggaatgcag cagtaaaacg tgctggtatt cgccgccgta atccgtacca
     7861 tacgcggcat acttttgcct gctggctgtt gacggcagga gcgaacccgg catttatagc
     7921 cagccagatg gggcatgaaa ctgcgcagat ggtgtatgaa atttacggta tgtggattga
     7981 tgatatgaac gacgaacagg tagccatgtt gaatgcgcgg ctatcgtagt tgcaaagttt
     8041 gcccccaatt tgccccattt agtaccagag aaccgaaata atgcaagaaa atcaaaagaa
     8101 tacgaagaaa gaacaataca acctgaacaa attacaaaaa cgtctgcgtc gtaacgtggg
     8161 cgaagccatt gctgacttca atatgattga agaaggcgat cgcatcatgg tttgcctctc
     8221 cgggggtaaa gacagctata ccatgctgga gattctgcgc aatttgcagc aaagcgcgcc
     8281 aatcaatttt tcgctggtgg ctgttaacct cgatcaaaag caaccaggct tcccggaaca
     8341 cgttctgccc gagtatcttg aaaagctggg cgttgagtac aagattgtcg aagagaacac
     8401 ttacggtatc gtgaaagaga agattccaga gggcaaaacc acttgctcac tgtgttctcg
     8461 ccttcgtcgc ggtatccttt atcgtaccgc aacggaactg ggggcgacga agatcgcgct
     8521 gggtcaccat cgtgacgata tcctgcagac gctgttctta aatatgttct acggcggcaa
     8581 gatgaaaggt atgcctccga aactgatgag cgatgatggc aaacatatcg ttattcgtcc
     8641 gctggcctac tgccgcgaga aagatattca gcgctttgcc gatgcaaaag cgttcccgat
     8701 tattccgtgc aacctgtgcg gttcacagcc taacctgcaa cgtcaggtga ttgctgacat
     8761 gttgcgtgac tgggataaac gttatccagg gcgtatcgag acgatgttca gcgcgatgca
     8821 gaatgtggtg ccgtcgcatc tgtgcgatac caacctgttc gatttcaaag gcatcaccca
     8881 cggttctgaa gtggttaacg ggggtgatct ggcgtttgat cgcgaagaga tcccactaca
     8941 accggcgggc tggcagccag aagaagatga aaatcagttg gatgagttac ggctgaatgt
     9001 ggttgaagtg aaataaccag gatagcgccc gatgcgcaag cgtatcgggc tactcttatg
     9061 gaggccggat aagacgcggc cagcgtcgca tccggcaatc ccgaataaga tgtttactct
     9121 tgcacccggc aattcaacat ttcattattt taataaccgc acccggcatg tttttccttt
     9181 aatcttcccg ccctgtaact gtttccatgc tttatgagca acagcctgac ggaccgcgac
     9241 atagacatgc gccggatgca cggcgatttt gccaatatct gcgccatcaa gcccgatatc
     9301 tcctgtcagt gcacctaata catcacccgg gcgcattttg gcttttttcc cgccatcgat
     9361 acacaacgtt gccatttctg cttccagcgg cacaatggaa ctattaactg gcggtgtttg
     9421 ccagttaagt gttatctgca acatgtcaga aatgatattg gcccgctgtg cttcttccgg
     9481 ggcacagaaa ctgatcgcca gaccgctatt tcctgcacga gctgtacgac cgatgcgatg
     9541 tacatgaact tcagggtccc acgccagctc aaagttcacc accagctcaa gcgatttaat
     9601 atccagacca cgcgcagcaa catcagtcgc aaccagtaca cgggcgctac cgttagcaaa
     9661 acgtaccaga gtctgatcgc gatcgcgttg ctccagatcg ccgtgtaacg acaatgcact
     9721 ttgccctact tcattcagcg cgtcgcagac agcctggcaa tcttttttgg tattgcaaaa
     9781 caccacgcaa gaggatggct gatgcaagct taataaccgt tgcaacagag gaattttgcc
     9841 tttgctggat gtctcataaa attgttgttc aatgggtggc aaagcatctg ttgagtcaat
     9901 ttcaatcgcc aaaggatcgc gttgcactcg tccgctgatt gcggcgatgg cttccggcca
     9961 ggttgccgaa aacagaagcg tctgtcgaga tgcaggcgca aaacggatga catcatcaat
    10021 ggcatcgcta aatcccatat ccagcatgcg gtcggcctca tccatcacca aagtattcag
    10081 cgcatccagt gataccgtgc ctttttgcaa gtgatccagc aaacgacccg gcgttgccac
    10141 gataatatgc ggcgcatgtt gcaacgaatc acgctgcata ccaaacggtt gaccaccgca
    10201 caacgtcaaa attttggtat ttggcagaaa acgcgccagc cgacgcaatt caccggccac
    10261 ctgatccgcc agttcacgcg taggacacag cactaaagcc tgggtttgaa atagcgacgc
    10321 atcaatttgc tgtaacaagc cgaggccaaa agccgccgtt ttgccgctgc cggttttcgc
    10381 ctgcacgcga acatcttttc cggcaaggat cgccggaagc gcggcggcct gcaccggcgt
    10441 catggttaaa taccccaact cattaaggtt cgtgagttgg gcaggaggca aaacattcag
    10501 ggtagaaaaa gcggtcacaa tctattctcg tggtcatcga cgcaaagtta gcaggcgcgt
    10561 atcctcgcag atctacgctc acgatgcgac aatttaatcg gttcttcatc gggtggtggg
    10621 tcaggcatgg gttgcgggcg agggatcgga tcgggcactg gaacaggatc gccaggaatc
    10681 ggttcaggga caggaatttg caaataaata agtgtcgtca tatttccctc cggtcattgg
    10741 gtggactctt aaagggtaga cgctgataaa taacaggcaa aaaaaagccg actcatcaaa
    10801 gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca aaaaaggaag taagacaata
    10861 tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc aagggagata ttgccctgaa
    10921 tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg tttttgtata cagacagccg
    10981 gaaaaattgc ttttgttaca accatttact acggtgcaac cataaagcaa caccaccaat
    11041 aagaacaact aacagaatac aaaaaattga aaatccgaat tgccacccgc cgccagggat
    11101 cccaccaagg ttgacgccaa ataacccggt cagaaaggta ctgggtaaaa agaccattgc
    11161 catcaacgac attgtatagg tacgacgagc taaattttcc tgcatcacct gagcgatttc
    11221 atccgccatc acgccagtcc gtgctataca ggcgtcgatt tcgtcaaggc cgcgcccaag
    11281 gcgatcggca atatcctgca tccgacggcg ttggtcatcg ctcatccacg gcaaacgttc
    11341 actggcaaga cgagcataaa catcacgttg cggtgccata tagcgacgca tcacaattaa
    11401 ttgtttgcgc agcagagcca ggaatccacg cggtggaatt tgctgatcaa ggagattatc
    11461 ttcaaggtcg ataattttat cgtgcagctg ctcgataaat tcgctggaat gatcggtcaa
    11521 cgcatcgcac acatccacca gccaccctcc gcaatcggtc ggacccgtgc cctcttccag
    11581 atcgctcacc acatcgtcca gcgccaacac tttgcgttgt cgggtcgaaa caattaaccg
    11641 cccgtccata tatacacgca tggcgaccag ttgatcgggg cgttcatcgg tgctgccgtt
    11701 tatacagcgc aatgtaatca gcgtgccttc accgagacgg ctgactcggg gccgtgtgct
    11761 ctcacctgcc agcgcatcac gtacgttatt gggaagcagc ggtgttgtcg ccagccattg
    11821 ggcactgtca ttgtgtacat aattaaggtg gagccagcag ggatgcgctt catcaatcac
    11881 atctgtattt tccagcggtt taacgccgcc tctaccatcc agcatccagg caaatactgc
    11941 atccgggaca ttaacgtccg atcccttaat cgcttccaca gtgcctccat catcaacgca
    12001 ttattttgta gtctagcctt ctggccctgt tacgcaacat ctcatcaccc cattaccctg
    12061 aaatgattaa taaaattctg tctaaattga atacaaaaag caaaatgctt ttccgtatac
    12121 aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga attatctgat catatgacgt
    12181 ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa taatgggatc tcaatgatta
    12241 cgcacaactt caataccctg gacttactca ccagtcctgt ctggatcgtt tcgccctttg
    12301 aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt gatgcaagac ctcacgttta
    12361 gtcagctacg aaccggtccc tattccgtct cctcacaaaa agaactgccg aaatacctct
    12421 ccgatctgca aaaccaacac gatattatcg aaatcctcac tgttcagcgt aaagaagagg
    12481 aaacagcatt aagctgtcgg cttgttttgc gagagctgac agaaacagaa ccggtgatta
    12541 ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa agccagtcgc tcggcaaatt
    12601 atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct gactaactct gcaccaatgt
    12661 tgttgattga cccgtcacga gatggacaaa tcgtcgatgc aaacctcgcc gcgctcaatt
    12721 tctatggtta taaccatgaa acgatgtgcc agaaacatac ctgggaaata aatatgctcg
    12781 ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt acccggcggt cataaacctt
    12841 tgaattttgt tcataaactg gcggatggtt ctactcgtca tgtgcagacc tatgccggac
    12901 cgattgaaat ttatggcgac aagctcatgt tatgtattgt gcatgatatt actgagcaaa
    12961 aacggctgga ggagcagctg gaacatgctg ctcaccatga cgcgatgacc ggattactga
    13021 atcggcgaca gttttatcac attacggaac caggccaaat gcagcatctc gccatcgctc
    13081 aggattacag cttgttgctc atcgacaccg atcgttttaa acacattaac gatctctatg
    13141 ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg caccctcgaa agttgcgctc
    13201 gcaaaggcga tttggtgttt cgttggggag gcgaagagtt tgtcttattg ctaccaagaa
    13261 ccccactgga taccgcgctt tcgctggctg aaactatccg tgtaagcgtg gcaaaagtga
    13321 gtatttcggg cttaccacgc tttaccgtca gcattggtgt ggcgcatcac gaaggaaatg
    13381 aaagcatcga tgaactgttt aaacgcgttg atgatgcttt gtatcgggcg aaaaatgatg
    13441 gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc tcgagatcaa cggctgcgct
    13501 tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt tttacgtagt gcgacgtaac
    13561 acgccccgct gccgccatga tgcggtagcg cggtgcaata tgcctgaaca tcatcaaatt
    13621 cggtcagcca gcgcgccaca tagctgcgga caatattggc atgcgattta tcatcccgtc
    13681 ctttgccatg aataatcagc acgttacgca aaccatcagc catggcttgt tgaatgaaac
    13741 tgaacatcat tttgcggcac tcttccaccg gctggcgcaa aagattcagg ctcgcctgtt
    13801 gcggatattt accactgcgc agcttatcca gcaccccatg ttgcaaccct tcccgccgaa
    13861 actccagcgg ctgacttagt gggatgatgt cgagaaatcc ggtggtgagg aaattatcaa
    13921 gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg cgttggatgc cagtggacat
    13981 cggtagcacg tttcagcggc tggacatctt ccatggcgtc aagaaacagc gatttgtcgt
    14041 caaggttcat gttacatcct cccgcaatta agagcgcgat atgataacca gaccgggtcg
    14101 gtccaacaac gtattaccca aaattccagt aataagttcc aaatattgcc gatattttaa
    14161 gcaaaatgct tatgcatgat tattcattca cgatattaat aatgtaactt atattttcgt
    14221 gaaatctgtc actgaagaaa attggcaact aaaggttaaa accgttataa cacagtcacc
    14281 ggcgcagagg agacaatgcc ggatttaaga cgcggatgca ctgctgtgtg tactgtagag
    14341 tctggcggat gtcgacagac tctatttttt tatgcagttt taactttgca gatagccgca
    14401 ttctcgccgg aattgcgtga ttaatgacgc ggtcagtggt gtctggcgac tatcacgccg
    14461 ctgaatcaaa taataggccg ctttcggtaa aatttcgcta accggcaaca tcaccagccc
    14521 cagtccgtgc aagggatcgc agcccatttc ttcaggcagt atgctgagaa aatcgctttt
    14581 tgccaccagc ctgatacagg ctgagaacgt ctcgcagact acaccgacct gtggcgtttg
    14641 cgcctgatcg tcaagcaatt cactcaactg tttgtagtag ctgccgtgtg gcgtcggcat
    14701 tgtccagctg taatccagta actgtttgat cgaacgggca ccaatggcgg ggtgtcccgg
    14761 gcggcaaaag atcgcgaatt gcttttccag taatttctca aaagtaaatt cgtggtcgta
    14821 cggtcccaga taataggtat tgatggtgaa atccaattct ccctgacgca attcattaat
    14881 catcgacacc agttgccctt ccataatgcg tacttttacc tgcggatgct gctgatgaaa
    14941 acgagatatg acagctggca tcagactgcg ggaaatactg gcccccatgc cgatattaat
    15001 ctgccctgcc agttgccctt gtcgttggcg aatatcctct tgggctgcgc gcagttcttc
    15061 aagaataaga ctggcgtgct gataaaaact ttcaccggca tcagttaacg tcacgccttt
    15121 actacggcga aaaaagagtt gcgccgctaa cccttcttcc agctcctgaa tagatttact
    15181 cagtgccggt tgcgacatat tcaacattcg gctcgctccg cgaatgctgc cctgacgagc
    15241 cacttcaaca aaagcccgaa tttgatgaat ttttacctga aaagccatga cgccatcgat
    15301 aaccgttatt tatcagacca aagaaactgg catctacttt aatgcagatg attgtgtcag
    15361 ggtaatttat gaacggttaa aactgtgaaa aatcagttag tgataagtaa aaactatcgc
    15421 tacgtgaacc gggtcacact ttttactgat gacgggaaag gttatggagt ctttgaatca
    15481 atttgttaat tcgcttgccc caaaattatc gtactggcga cgtgattttc atcactatgc
    15541 agagtctggc tgggtggaat tccgcactgc cacccttgtt gcggaagaat tgcaccagct
    15601 cggctattca ctggcgctgg gccgcgaagt agttaatgaa agtagccgga tgggactacc
    15661 tgatgaactc actctacaac gcgaattcga gcgcgctcgt caacagggtg cgctagcaca
    15721 atggattgcg gtttttgaag gtggtttcac tggtatcgtc gccaccctgg ataccggtcg
    15781 ccccggtccg gtgatggctt tccgtgtcga tatggacgcg ctggatctca gtgaagagca
    15841 ggatgtcagc catcgcccct accgcgacgg ttttgcgtca tgtaacgccg gaatgatgca
    15901 tgcctgtggt catgatggac ataccgccat tgggcttggg ctggcgcata cccttaaaca
    15961 attcgagtcc ggactacatg gcgtcatcaa actgattttt cagcctgcag aggaaggtac
    16021 gcgtggcgcg cgggcgatgg tcgatgcagg tgtcgtagat gatgttgatt attttactgc
    16081 cgtgcacatt ggcactggcg tacctgcggg caccgtggtg tgcggcagtg ataattttat
    16141 ggcaaccacc aaatttgacg cgcacttcac cggtaccgcc gctcacgcag gcgcaaaacc
    16201 agaagacggt cacaatgcct tgttggcggc agcacaagcc actcttgcac tgcatgcaat
    16261 cgccccgcac agcgaaggag cttccagagt aaacgtgggc gttatgcagg caggaagcgg
    16321 tcgtaacgtt gttcctgcct cggcgttgct gaaagtggaa acacgcgggg ccagcgacgt
    16381 cattaatcaa tatgtttttg accgtgcaca acaagcgatt cagggcgcag caaccatgta
    16441 tggtgtcggc gttgaaactc gtctgatggg tgcagctacc gccagttctc cttcgccgca
    16501 atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg ggggtcaatc aggccattga
    16561 acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta atgatggccc gcgtgcagca
    16621 acatcaaggg caagcctcct acgtggtgtt tggcacacag ctggcggcag gtcatcacaa
    16681 cgaaaaattc gattttgacg agcaggtttt cgctattgcc gtcgaaacgc tggcgcgcac
    16741 cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg aaatctatcg ttttatcgac
    16801 gatgcgattg aagccgatcg ccaacgttat accgatattg ccgatcaaat ctgggatcat
    16861 ccagaaacac gttttgaaga gttctggtca gcggagcatc tggcttcggc gctggaatct
    16921 gcaggcttca ccgttacccg caacgtaggc aatatcccaa atgcctttat tgcttcgttt
    16981 ggtcaaggca aaccggttat cgccctgctg ggagaatatg acgccctggc aggtttaagt
    17041 cagcaagcag gttgcgcgca acctacatcc gtgacgcccg gtgaaaatgg tcacggttgc
    17101 ggacacaatt tgctgggaac cgccgccttt gccgctgcaa tagccgtcaa gaaatggctg
    17161 gaacaatatg ggcaaggcgg cacggtgcgc ttttatggtt gtcctggcga agaaggcggc
    17221 tcgggtaaaa cgttcatggt tcgcgagggg gtatttgatg atgtggatgc ggcactcacc
    17281 tggcacccgg aagcctttgc cggtatgttc aatacccgca cgctggcaaa cattcaggca
    17341 tcatggcgct ttaaagggat cgcagcacat gccgcgaatt cccctcattt gggacgcagc
    17401 gcccttgatg ccgtaacgtt gatgaccact ggcaccaact tcctcaacga acatattatt
    17461 gaaaaagcgc gcgtacacta tgccatcaca aatagcggcg ggatctcgcc caacgtggtc
    17521 caggcgcagg cagaagtgct ttatcttatc cgcgcccccg aaatgaccga cgtgcagcat
    17581 atttatgatc gggtcgccaa aatcgccgaa ggtgcggcat tgatgaccga aaccacggtt
    17641 gaatgccgct tcgacaaagc ctgttccagt tatctcccga atcgcacctt agaaaatgcc
    17701 atgtaccagg ccctatccca ttttggtacc ccggaatgga actccgaaga actggctttt
    17761 gcgaaacaaa ttcaggctac gctcaccccc aacgatcggc aaaacagtct gaataatatc
    17821 gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac gtcatcgtga aacggtactg
    17881 gcgaatgagg tcgctccata tgccgccacc gataacgtgc ttgcggcatc gactgatgtc
    17941 ggcgacgtca gttggaaact gcctgttgcc cagtgtttca gcccctgttt tgccgtcggt
    18001 acaccgctac atacgtggca actggttagc caggggcgaa catctattgc tcataaagga
    18061 atgctgctgg cggcgaaaac tatggcagca accacagtca atctcttcat tgattcaggg
    18121 ctattgcaag aatgccaaca agagcatcag caagttacgg acacgcaacc gtatcactgc
    18181 cctatcccga aaaacgtgac accgtcacct ttaaaataac aacaacaacg caaacacaac
    18241 aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt cctcccaatc cgggaagctc
    18301 tatggctggg tcgaaagaat tggtaacaag gttccccatc cttttttgct ctttatctat
    18361 ttgattatcg tactcatggt gacgacggca attttgtcag cctttggcgt cagtgcgaaa
    18421 aacccgaccg atggtacgcc ggtggtggtg aaaaacctgc tcagtgtaga aggattacac
    18481 tggtttttac ccaatgtgat taaaaacttt agcggttttg ctccacttgg agcgatcctg
    18541 gcgctggttt taggtgccgg cctagcggag cgcgtcggct tactgccagc gctaatggtt
    18601 aaaatggcat cgcatgttaa tgcccgctac gccagttata tggtgctgtt tattgctttt
    18661 ttcagccaca tttcttccga tgcggcgtta gtgatcatgc caccgatggg tgcgctgatt
    18721 tttctggcgg tgggcaggca tccagttgca ggtttactgg ctgccattgc aggcgtaggt
    18781 tgcggcttta cggctaattt actgattgtc acaaccgacg tgttgctgtc ggggatcagc
    18841 acggaagcgg cggctgcgtt caatccgcaa atgcacgtca gtgtaattga taactggtat
    18901 tttatggcca gctccgtagt cgtactgacg attgttggcg gcctgataac cgacaaaatc
    18961 atcgagccac ggttaggtca atggcaggga aacagcgatg agaaactgca gacattgacc
    19021 gaaagtcagc gttttggttt acgcatagca ggtgtcttat cgctactttt tattgctgcg
    19081 attgcgctga tggtgatccc ggaaaacggg atattgcgcg atccgattaa tcacaccgtg
    19141 atgccatcac cctttattaa aggtatcgtg ccactgatca ttcttttttt ctttgttgtc
    19201 tcgctggctt atggcatcgc tacccgcaca attcgacgtc aggcggattt accgcattta
    19261 atgattgaac cgatgaaaga gatggcggga tttatcgtga tggtttttcc cctcgcccaa
    19321 tttgtcgcca tgtttaactg gagcaacatg gggaaattca tcgccgtggg gctgaccgat
    19381 atactggaaa gttcagggct tagcggcatc ccggcgtttg tcggtctggc gttgctttcc
    19441 tctttcttat gcatgtttat tgccagcggt tccgcaatct ggtcgattct tgcccccatt
    19501 ttcgtaccaa tgtttatgct acttggcttt cacccggcat ttgcgcaaat cctctttcgt
    19561 attgccgact catccgtatt gcctttagcg ccggtatctc cttttgttcc actgtttctt
    19621 ggattcctgc aacgctacaa accagacgcg aaactgggta cttactattc gttagtcttg
    19681 ccctatccgc ttatcttttt ggtggtatgg ctgctgatgt tgctggcgtg gtatcttgtt
    19741 ggtctgccga taggtccggg gatttaccca cgtttgtctt aagagagaac ggatgctgag
    19801 attacttgaa gaaaaaattg ccacgccact ggggccactg tgggtgattt gcgatgagca
    19861 atttcgtctg cgggcggttg aatgggaaga gtacagcgaa cgcatggtgc agctgctgga
    19921 catccattat cgcaaagaag gctatgagcg catttctgcc accaacccag gcggtttaag
    19981 cgacaagctt cgtgattatt ttgccggtaa tcttagcatt attgatacgc ttcccactgc
    20041 cacagggggg acgccatttc agcgcgaagt ctggaaaaca ctacgcacta tctcctgcgg
    20101 gcaggtaatg cattacggcc aactggctga acaattgggc cgccctggcg cggcgcgtgc
    20161 cgttggtgcg gcaaacggat cgaatcccat cagcgtcgtc gtaccttgcc atcgggttat
    20221 tggccgaaac ggcaccatga ccggatatgc aggcggagtt cagcgaaaag agtggttatt
    20281 gcgccacgaa ggttatcttt tgctgtaaac attaaacaat ttgtgccagc ttgttcacac
    20341 ttttatgtaa agttaccctt aacaacttaa ggtttttcaa atagatagac atatatttac
    20401 atctaatatc ggaattctct gctgttaagg tttgcttaga cttacttgct ccctaaaaag
    20461 atgttaaaat tgacaaatat caattacggc ttgagcagac ctatgatccc ggaaaagcga
    20521 attatacggc gcattcagtc tggcggttgt gctatccatt gccaggattg cagcatcagc
    20581 cagctttgca tcccgttcac actcaacgaa catgagcttg atcagcttga taatatcatt
    20641 gagcggaaga agcctattca gaaaggccag acgctgttta aggctggtga tgaacttaaa
    20701 tcgctttatg ccatccgctc cggtacgatt aaaagttata ccatcactga gcaaggcgac
    20761 gagcaaatca ctggtttcca tttagcaggc gacctggtgg gatttgacgc catcggcagc
    20821 ggccatcacc cgagcttcgc gcaggcgctg gaaacctcga tggtatgtga aatcccgttc
    20881 gaaacgctgg acgatttgtc cggtaaaatg ccgaatctgc gtcagcagat gatgcgtctg
    20941 atgagcggtg aaatcaaagg cgatcaggac atgatcctgc tgttgtcgaa gaaaaatgcc
    21001 gaggaacgtc tggctgcatt catctacaac ctgtcccgtc gttttgccca acgcggcttc
    21061 tcccctcgtg aattccgcct gacgatgact cgtggcgata tcggtaacta tctgggcctg
    21121 acggtagaaa ccatcagccg tctgctgggt cgcttccaga aaagcggcat gctggcagtc
    21181 aaaggtaaat acatcaccat cgaaaataac gatgcgctgg cccagcttgc tggtcatacg
    21241 cgtaacgttg cctgattttt ccgcataact cactatcctt ctgtcatatc attaaatttt
    21301 tctgatttat tgatctggca gaaggttcat cactgtttca ttcaccagat atgggttaat
    21361 cttttaatta caaactgcgt tgacagttgt tgtaaggaga ccctgtatgg ctatgtatca
    21421 gaacatgctc gttgttatcg atcctaacca ggacgaccaa ccagcattgc ggcgagctgt
    21481 ttatttacat caacggattg gtggcaaaat taaagccttt ttgccgatct atgacttctc
    21541 atacgaaatg accaccctgc tctccccgga cgaacgtacc gctatgcgtc agggcgtcat
    21601 cagccagcgt acagcctgga tccacgagca ggcaaaatat tatctcaatg ctggcgttcc
    21661 cattgaaatt aaagtggtct ggcataaccg tcctttcgaa gccatcattc aggaagtgat
    21721 cagcggcggg cacgatttgg tgctaaaaat ggcgcaccaa catgaccgtc tggaagcggt
    21781 gatttttacg ccaacggact ggcatctgtt acgcaaatgc ccaagcccgg tgtggatggt
    21841 gaaagaccag ccgtggccgg aaggaggtaa ggcgctggtg gcggtgaatc tcgccagtga
    21901 agagccgtac cataatgcgc tcaatgaaaa actggtcaaa gagacgatcg aactggcaga
    21961 acaagtcaac cataccgaag ttcatctggt tggcgcttat ccggtaacgc caattaatat
    22021 cgcgattgaa ctgccggaat ttgatccgag cgtttataac gatgccattc gtgggcaaca
    22081 tttgctggca atgaaagctc tgcggcagaa attcggcatt aatgaaaaca tgacgcacgt
    22141 agaaaaaggt ctgccagaag aggtgattcc tgatttggcg gagcacttac aggcggggat
    22201 tgtggttctg ggcacggtag gacgcaccgg tatttcagca gcattcctcg gcaacacggc
    22261 ggaacaggtg attgatcatc ttcgctgcga tctgttggtc attaaacctg accagtatca
    22321 gacacccgtt gaactggatg acgaagaaga cgattaacga ctcttcaata actgacaagg
    22381 cccgagttat cgggcctatt ttttacttac tgctcttcgc cgccaaggaa ataaattccc
    22441 aacgggattg ccagcaacac agtaaagacc aggctataaa caaaaatcat cgatgactga
    22501 atcacataca ttgatgtcgt ccactcagac agcggaatat tgtactgttc tataacgccg
    22561 ccgatagtcg cgcgagtcac aaccgacgcc gcaataatga aaaccgccaa caaaccaaag
    22621 agaaatttct tccctttcgc tgacttcagt ttcgccataa tcatgatgta atccttgtgc
    22681 agacaaattt cttgttacct aaacgtaaca ataccatgac atttcaccgt tgtctgctct
    22741 gaaaacctca cctcttagct ggatttacaa tcaacgcatt gattagttag ctaatgttgc
    22801 tacttccttc cctgactatt gatgatctga atgaattgtc gcggtaaaat agccccgcca
    22861 ggggtttccg gcatgacgac aattatctgg aggagcctga taaaacaatg atcgctgaac
    22921 tgtttacaaa taatgcgctt aatctggtca ttattttcgg tagctgcgca gcattgattc
    22981 tgatgagctt ttggtttcgc cgcggaaatc gtaaaagaaa aggtttttta ttccatgcgg
    23041 tgcaattttt aatctacacc ataattatca gtgctgttgg tagcattatt aattatgtta
    23101 tagaaaacta taaactcaaa tttatcaccc caggcgttat cgattttatc tgtacgtccc
    23161 tgattgcggt tattctgacg attaagctat ttttgctgat taatcagttt gaaaaacagc
    23221 agattaaaaa aggtcgcgat atcaccagtg cgcggattat gtcgcgcatt atcaaaatca
    23281 ccattattgt ggtgcttgtt ctgctttatg gcgaacattt cggcatgagc ctttctggct
    23341 tgctgacctt tggtggtatt ggtggtctgg ctgtcggtat ggccggtaaa gatattctga
    23401 gtaatttctt ttccgggatc atgctctatt tcgaccgtcc tttcagtatt ggcgactgga
    23461 tccgttcacc ggacagaaat atcgaaggta cagtagcgga aattggctgg cgaattacca
    23521 aaattacgac ctttgataat cgtccattgt acgtaccgaa ctcgctgttt tcgtcgatca
    23581 gcgtagaaaa cccaggacga atgaccaacc gccgcattac cacgaccatt ggtttacgtt
    23641 atgaggatgc ggcaaaagtg ggcgttattg tcgaagccgt acgtgagatg ctgaaaaatc
    23701 acccggccat cgaccagcga caaaccttac tggtttattt caaccagttt gccgactcgt
    23761 cattgaatat tatggtttat tgctttacca aaaccacggt atgggctgaa tggcttgctg
    23821 cacagcaaga cgtttatttg aagattatcg atattgtgca gtcacacggc gcggactttg
    23881 ccttccccag ccagacgctg tatatggata acattacacc gccggaacag gggcgctaaa
    23941 tcagacatca ctggcgttat ccacaagggt aacgccagtt cccatcacat caatgcttca
    24001 caatatacat agtccggctg tacgcaacat cttccgggtt attaatggga taacctttca
    24061 gccacggctt gattaatcgt ccattggtat attgataaat tggtgcaatc ggtgcttgct
    24121 ccatgaggat tttttctgcc gcgttgtaat cggcattacg cgctttaacg gtattttccg
    24181 tcgatgcctg ggccagaact ttgtcgtatg ccggattgtt aaagcgtgaa atatttcctg
    24241 aatgcgttga cgttaataat gtcaggaaag tggagggttc attataatcc cccacccatg
    24301 aggcgcggat aacatcaaaa ttgccggtgt tacggctatc gatataggtt ttccattcct
    24361 gattttgcaa tttaacatct acgccaaggt tctttttcca catcgatgct acagcaatcg
    24421 caattttttg atggttttct gaagtgttat acaaaagcgt cagcttcagc ggtttttgcg
    24481 gaccataacc agctgcgctc aacaaagttt ttgcctgcgc attcagttct tcctgactca
    24541 tttgttcaaa cggcgaaggt tccggcgtaa atccggcggt aacatctggt gtaaaatgcc
    24601 acgctggctt ttcgcccgtt cccaacactt tttcggtcat caggcggcga tctatcgtca
    24661 tacttaatgc caggcgaacg cgctgatctg ccgtcggccc tttttgcgtg ttaaacgcat
    24721 aataataggt cccgagctgc ggcggcgtat aaacctgccc cggaatatcc ttcaacagct
    24781 tctgatacat atttttcggg aaggattcgg tgatatcaat atcccccgcg aggtaacgct
    24841 tagtggctgc ggattcctga ttaattggca ggaaagtcac tttttgcagc accgttttgg
    24901 cattatccca ataatgggta ttcggtacca cgaccagttt ttcattgact acgcgatctt
    24961 taagaacata agcgccattg ccgatcagat ttccggattt cgtccactct ttaccgcttt
    25021 ctacgttggc tttttgcacc gggaagaagg caaagttagc ggttaaattc acaaaccacg
    25081 gcaacggttt atcaagctga attttcaaag tatgggcatc aactgcggtg acgccaagct
    25141 ggtctggcgt agctttacca tcaataatcg cctgtgcgtt gttgattccc gccagcgcgg
    25201 caaaccatgc aaatggcgat agcgttttcg ggtccaccag acgttgccag ctgtagacaa
    25261 aatcttgcgc cgttaccggt gtgccatccg cccattttgc gttatcgcgc agggtaaaag
    25321 tccagatacg gttgtcatta cttttccact gagtcgcaac gccggggaca atctcccctt
    25381 tctcgttctg attcaccagt ccttcaaaca gatcgcgaat gacctgaatc tctggcaggc
    25441 ccacggcttt agcgggatcc agcgacgcag gctcatcttt aatgtggcgc accagctcct
    25501 gcttctctgc cagtactgtg ccgctcggaa cttctgcagc atacgaaaga gaaatgctgc
    25561 tgaccaacag cgcacaacac gtgactgaaa cagagtgctt cataagtttc cctctaaccg
    25621 acattaaaat tgatgcgtaa ttatttgttt cgccttaaag aaatgcaaac aacttccatc
    25681 aaaaaagtga cacaggaagc ataaaatgtt ttatgtatca atatattaaa tcagatttag
    25741 tgtaaataat cgacgccaaa atgcggtaaa gcacatagaa caccgaacac tacgttcagg
    25801 cttcgcacgt tcaaaccagt tttcggtatc ataatcagca tctgtcgcca ttgtacgaag
    25861 aataaaaaat aaccaaaacg ttgaaaggaa ctgagaatac gttaagagag aaattaaatt
    25921 aatgtatcag caagtagtgc taaacagcac ctttatactt cagcctatct ataaataatg
    25981 agaatgccga gccagcatgt cgacggtgtg gatagtacag gtgataaccg ggtaaatctg
    26041 gtgtgaattt atccaaaaca cgtatcagtt ttttttcttt aatagcccgc tcaacctgat
    26101 cataaggtag atacgccaat ccatgcccat caattgcagc atcaatgatc aggtctatcg
    26161 tattcagtaa aagctgacct tccatgcgaa cacgaacttc acgtccaccg cgtattaatc
    26221 tccagcgatt tgctgtaccc gatgtgggaa gatacaaatt aattgcctga tgatctatta
    26281 attgtgacac tgacgttgga acacttcggc gagaaaaata atccggtgag ccaacaatag
    26341 ccattggtat atctggcccg attcgaatgg cgatcatatc tttatccatc tccccaccca
    26401 gacggacgcc tgcatcaaaa cgttcagaaa cgacatcggt caaaccataa tcaatggtga
    26461 gctgaatatc aatttcagga tgcgatttca ggaatgtgcg cattgctggt aacaatatcg
    26521 tttttgctgc atgttctaca gtagtaatac gtattgtccc ggatgggcgg ttctgcagct
    26581 cgctcaggga tgccagggct gaatctatgt catgcaacat agggccaaga acagacaaaa
    26641 gatgctcgcc cgcttcagtt ggaacaacgc tgcgcgtggt tcgcgtcaga agccgcaatc
    26701 ccaatcgttc ttctatacga cgcactatct ggcttaaagc tgactgcgcc atgctcaggc
    26761 gggctgctgc acgagtgaag ctacgctcct ctgcaacgac gacaaacgcc atcagatcag
    26821 cgatttcttc acgcttcatt cagtatctcc atgcaatcta ttaatcattt aaatgatagg
    26881 tctaatcatg attttgggtc taatcaactt aatgatcaat atctatagtg atatcaccaa
    26941 ccgttaaaac gaaccgacaa ctccatgagg aacggaatga tgaataataa agtcagcttc
    27001 actaacagca ataatccaac catctctttg tctgcagtga tctatttccc tccaaaattt
    27061 gatgaaaccc gtcagtatca ggcaattgtg gtatctcacc caggtggggg cgttaaagaa
    27121 caaacggccg gaacctatgc caaaaaactg gcggaaaagg gatttgtcac tattgcttat
    27181 gacgcatctt atcaaggtga aagtggcggc gagccgcgtc agttagaaaa cccatacatt
    27241 cgtactgaag acatcagtgc cgtgattgat tacctgacaa cgctttctta tgtcgacaat
    27301 acccgaattg gtgcgatggg aatttgtgcc ggtgcaggat atacagccaa tgctgcaatt
    27361 caggatcgcc gcatcaaggc catcggtacc gttagtgcgg tcaatattgg ttcgatgttc
    27421 cgtaatggtt gggaaaacaa tgtgaaatct attgatgccc tgccatacgt tgaagcgggt
    27481 tcaaacgccc gaaccagcga tattagcagc ggagaatatg ccgttatgcc actggcacca
    27541 atgaaggagt ccgatgcgcc gaacgaagaa ctgcgtcagg cctgggagta ttaccacacc
    27601 cctcgcgcgc agtatccaac agcaccgggt tacgctactt tgcgcagcct taaccagatt
    27661 attacctatg atgcttacca tatggcggaa gtgtacctga ctcagccgat gcagattgtg
    27721 gcgggcagcc aggcagggag caaatggatg agtgacgacc tgtacgatcg ggcatcaagc
    27781 caagataaac gctatcacat tgttgaaggc gcaaaccata tggatcttta tgacggcaaa
    27841 gcctatgtgg ctgaagcaat ttcagtatta gctccgttct ttgaggaaac tctgtaatga
    27901 aacatattca aatccgcaat agtgatatgg cctggcatat tgctgccaat attcagtttc
    27961 caccaaattt tgatgagtca aaacagtatc cggcgattat cagcgtccat ccttttggca
    28021 gttgtaagga gcagacgtct ggcaatatct atggcaaagc attggctgaa aaaggctatc
    28081 tcgttctcgc ttatgacgcc agtttccagg gagaatctgg tggggagcct cgctggatag
    28141 aagatccgac acagcgcgta gaggatatta gccgtgttat cgattatgca gtaactctgc
    28201 catatgttaa tgctgaacgt attggcgtgc tgggagtgtg tgggggtggt ggctatgcca
    28261 ttaatgccgc gctgacagaa aaacgaatta aagcagtagt gagtattacg ggtgttaata
    28321 tcgggcgtct ttttcgggaa ggtttcagca attacgatcc gattggcgca ttgaatgcaa
    28381 tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac gaattacttc
    28441 ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt tatgaagcaa
    28501 ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa atgttgttct
    28561 cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg ttactgactc
    28621 agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat cgtgatggac
    28681 tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca ttacccgatt
    28741 tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg gaaaaagtta
    28801 ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga aaaatgtact
    28861 gattcttggt gctggcggtc agatagccca ccatgtgatt aatcaactgg cagataaaca
    28921 aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac cctatccaac
    28981 taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc aggccatgca
    29041 gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc aggcaaattc
    29101 agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc tttccctcgg
    29161 tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta ttggtgagcc
    29221 cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg aatatacaat
    29281 tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta cctcacgcaa
    29341 tgaaccattc aaatgtacga tcgtatcacg taagagtgtc gcagcattaa tcacagatat
    29401 catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaat ccgtgactga
    29461 tggtgacaag ccattattca tgtaaatcaa ttacatagtt accatcgtaa tgtactatgt
    29521 gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga ttaacatgtg
    29581 tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca tctaagtcag
    29641 cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa aaaaagtgat
    29701 acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa tttgcacaac
    29761 actgcctgta atacttcctg aaccagagac gatcatgacc gtaacccgcc cacgcgccga
    29821 acgcggcgca tttccgcccg gaacagaaca ttacggacgt tcattattgg gcgcgccgtt
    29881 aatctggttt cctgcccctg cagccagccg cgaaagtggt ttgattctgg ctggcactca
    29941 cggtgatgaa aactcttcgg tcgtcacgct ctcctgtgct ctgcggacgt tgacaccttc
    30001 tttgcgccgc catcatgtgg tgctgtgtgt gaatcctgac ggctgccagc tggggttacg
    30061 ggccaatgct aatggtgtgg atttaaacag aaactttccg gcggcgaact ggaaggaagg
    30121 tgaaacggtt tatcgctgga acagcgccgc tgaagaacgt gatgtcgtat tactgacagg
    30181 tgacaaacct ggctcagaac ctgaaaccca ggcgctgtgc cagcttatac accgcattca
    30241 acctgcctgg gtggtctcat tccacgatcc actggcctgt attgaagatc ccagacacag
    30301 cgaattaggc gaatggctgg cccaggcgtt tgaattgcct ctggtaacca gcgtcggtta
    30361 tgaaacgcct ggctctttcg gcagttggtg tgcagatctc aacttgcatt gcatcaccgc
    30421 agagtttccg cccatctcct ccgacgaagc cagcgaaaaa tatctctttg cgatggcaaa
    30481 cttgctgcgc tggcatccta aagatgcaat tcgcccgtcg tgaactgaag cgccggttcc
    30541 acatctaccg ccagccaggt cggtccgtca agatcggcga aactgacctg cggcaccagc
    30601 ggtaaagcgg cgctaatggc acgagaggta cacaacatgc agcccagcat cagacgaaaa
    30661 ccttgtgcac gtgcttcagt cgccagcgcc agcgcttccg tcagaccccc ggttttatcg
    30721 agcttaatgt taaccatctc atagcgccct ttcagcgcct tcagattgct acgagtatga
    30781 caactttcat cagcacaaat cggcaacgga tgaataaaat tctccagtgc cgcatcgtcc
    30841 tgcgcaggaa gcggttgttc aagcatcgca acgcctaaat ccgccaatag ctggcaacgc
    30901 gccgccaacc cttctgcacg ccaggattca tttgcatcaa cgatcagcgt cgcatcgggc
    30961 acagcggtgc gaattgccac catccgctca ctgataagat ggttatccag cttcactttc
    31021 agtaatttcg cgcctgcctg ccagagtgtt gatgcactat tggccatctg atcaggcgta
    31081 ccgatgacaa ccgtctgtgc agtgataact gtcccaggaa gcgttatgcc gatcaaatca
    31141 gccagcgatt gctgctgttt tcgcgccgcc aaatcccaca atgcacaatc cagcgcatta
    31201 cgtgctgcgc cggcagggag aattttttgc aactcctccc gtgtcagccc tttctctaat
    31261 tgcggtacga cgctcataat ttgcgccatt accgaggcat cactttcccc ataacgcgga
    31321 tacggcgtgc attcgccggt gcctttaata ccctcttctt ccagttcaac caccaccacg
    31381 cgcgcttcac tgcgacttcc ccgggcaatc acaaacgggg tatgtaaggg ccaggcttcc
    31441 tcgaatacct taacggttct catctttact ccttgttgtc gcggtggtct gcggattaag
    31501 cgcaaatagc gtttgctgtg ttattgacag ttagcataaa ctaggtgtga cgttaactat
    31561 atgtaaacag gaaagataat catgtcacaa accgttcatt tccagggcaa cccggttaca
    31621 gtcgccaatt ccatcccgca ggcgggtagc aaagcgcaga cttttactct cgtagcaaaa
    31681 gatctgtctg acgtcaccct cggtcagttt gcgggtaaac gcaaagtgct gaacattttc
    31741 ccgagtattg ataccggagt ttgcgccgca tcagtacgta agtttaacca actggcaacc
    31801 gagatcgaca acaccgttgt gctgtgtatt tctgccgatc tgccgttcgc ccagtctcgt
    31861 ttctgcggcg cagaaggtct gaacaacgtt atcaccctct ccactttccg taacgctgaa
    31921 ttcctgcaag cctacggtgt ggcaattgct gatggcccac tgaaaggtct ggcagcgcgt
    31981 gccgttgtgg ttattgacga aaatgacaat gtgattttca gccagctggt ggatgaaatc
    32041 accaccgagc cggattacga agcagctctg gctgtactga aagcataatt taatatgcct
    32101 gatggtgttg caccatcagg catattcgcg cttactcttc gttcttcttc tgactcagac
    32161 catattcccg caacttattg gcaatcgcgg tatgtgaaac gccgagacgt tttgccagtt
    32221 tgcgcgtgct gggataattg cgataaagct gggttaatac cgagcgttca aaacggctgg
    32281 tgatttcgtc cagcgaacct tccatcgcat cttcgcccac ggctaccgtt gcggcgtcat
    32341 aatccggcaa caaaatatcc tgtggacgca gctcataacc gtccagttgt gtcagtgcgc
    32401 gatagatagc gttctttaac tgccgcacat ttcccggcca cgcataacgc gtaagtacag
    32461 tattcaggtc agcggccagt ttcggacgcg gcacgccctg ctcgtcggca aagcgggcga
    32521 cgaacagctc agttaacggc atgatgtcct gcggacagtc acgtagcggc ggcagattga
    32581 gcgtcaacac gttcagacga taatagagat cttcacggaa catgcctttt tgcaccagtt
    32641 cgaccagatt cttctgcgta gcgcaaatca cccgcacatc gacatgcacc tcatggtctt
    32701 cgccaacccg acggaaagtg ccatcattaa ggaaacgcag taatttcgcc tgcatccgtg
    32761 gtgacatttc ccctatttca tccaacagca ccgaaccacc gttcgcctgc tcaaagaatc
    32821 ctttcttccc ttccggagca tgaccaaaca gttcactctc gaccgcatct tccggtatag
    32881 acgcacagtt cagcgccagg taaggtttgc ccgctctggg gcttgcctga tggcaggcgt
    32941 aggcaaagag atctttacct gtacctgtgt cacccgtaat cagcagcggc gcgcttagca
    33001 tcgccagttt ctgcgcctgt tcgacaacat gcttcatttt cgggctgacg gcgacaattt
    33061 gactgaaggc gctgacgtcc tgggcggcga cattttgcaa ctggcggccc atacgaatcg
    33121 ttgatcgcaa catcaccacc gcaccggtca ggacgtgttg atcattttca tcctgaagat
    33181 aaacaggcgt aatctccatc aggaaattct gcccattaat aacgacatgc tcgttatggg
    33241 aatcttgcgg ttcgctttcc agccaacgta aaaaattaaa gccgttaatc aattgtgcgg
    33301 cggtatggtt gcgcaggcga tccaattttt gcgcaaaaag ctgacagctc gccgggttcg
    33361 ccatatccac tttgcttttc atatcgacag agagcacagg ttcgggcaac gcctccagta
    33421 acgcgctcaa cgccagatgc tcacgttcgg aaggcatcca cgggacagta cgcacatcgg
    33481 taacacctgc aatacggcgt atttcggcca tcagactgct gaaactctca aactccagtt
    33541 cagcaaaatt gaggtagatt cgcccaatgg gatcaatctc aataccgcgt aaatcaatgc
    33601 ctcttagcac gagtagatcg agtaattcgc gggtcagacc gagtcggtct tcacaaaaga
    33661 cttccagacg catgggaacc ttcacctgaa aaaagaacaa ttaatatgat gatatgacac
    33721 tatttggata gcaggaaggt ttctgtcaac aatcattgac acaaagacgg aaaaacgggc
    33781 actttggcgt aaagatatcc ggtcagcacc cgatattgca ttatttttcg ctgggtgttt
    33841 tgcctttttg cagcgtttct ttcacctgac cgataagctg acgacggaaa tccccgaggc
    33901 gaggtttgtc atcgtcaatc cacggcagcg ggcggcaaag ctccatagct ttaatcccga
    33961 gtcgtgccgt cagaagtcca gcaccaattc cctgagctgc gcgggtagac aaacgagcag
    34021 cgagatcttg cgacatccag tccatcccca cttcgcgcac cagttcgctg gctccggcaa
    34081 aagcgatatt cagcaatacc agcttaaaca gacgcaaacg gctgtaatac cccagttcaa
    34141 tgccatacag cgtggcgatg cgattaatta aacgcagatt gcgccaggcg ataaacgcca
    34201 tatcgaccaa cgccagcggg ctgaccgcaa tcatcaacgt tgattccgcc gccgaacggc
    34261 tgatttcgcg ccgcgcctgg gcatctaaaa ctggctggac caaatgcgca tacaaactga
    34321 ccacttcacg gtcgttttgc gtttcatgga ttgaggcata ccagcgttgc agcgctggat
    34381 gagactgatc aatacccgcc tgctgcgcca gtttttcgca aaatgcgcgg cctttgcccg
    34441 tgccgtggct gtgcaacaaa tcgcgcgctt cgtcgcgttc atgggcgcgc tgtcgcaagc
    34501 gccataagcg ccgccactct gttaccacag aacctacgcc agcgccgata atcaatgccc
    34561 cagcggcaca tccacccagc gccacccagt cctgagtttg ccaggcattc attgtccact
    34621 gtacaccctg cccgacaacg cttgcgccaa acagagccag cccgcccatc accattttgc
    34681 gccacaggct gcgtttcgga cgtaacgctg catccattac cgcttcaact tgcccctctt
    34741 cttcaggcgc ttcatcgagc gtggccgggg caaaattttg cgcctgattt tcgtcaaagg
    34801 tctgctgcgc cctgaatttt ggattctgat cgacctccag cggaccgtcg aaatcaatac
    34861 gtggttttaa cggttcggtc atcgcaattt atctcctatt aaaaattcaa gcgcggcatc
    34921 aagacgaata tgcggcagcg gtttgtcgac atccatcacc tgcgggcgaa acgcttcaaa
    34981 ctggaaccct tgcttgtccc agaacgcctg accgggcaaa cgcgcaggaa cttcaccggg
    35041 ataaacggtg agtggcgcac catcgctgag ccggttaccg cgcaatgccg gaattttctc
    35101 gccgttgacg tcaataatgc cgctggtggt cgcctgcacc gaggccagcc ccaggcaatc
    35161 catactaatc ccttcaaacg ccgcattttg ccaggcatcc tgaatcagtt gttgcagtaa
    35221 tgaaaccata ttggcgtgct gatcgatggt cacatggtcc gctttagtgg cagcaaacaa
    35281 tagcttatcg ataaccggcg aaaacaaacg ccggaacaag gtacgctgcc cgtagtggaa
    35341 actttgcatc agctgcgtca gtgccagacg catatcatta aatgcctgtg gcccactgtt
    35401 gagaggttgc aggcaatcaa ccagcacaat ctggcggtca aagcgcagaa aatgattctt
    35461 atagaacccc ttcaccacct tctcgcagta ataattaaac cgctcgcgca gcattccggc
    35521 attggtgtgc ttatcggcct gcgccagttt ggactcgccc caggcatcga catccggcca
    35581 cgggaagaat tgcagcgcgg gcgcacctgc catatctccc ggcaagacaa agcgcccagg
    35641 ctgaataaag tgcagcccct gctgtttaca gtggtggaga taatcggtcc acgcggcggc
    35701 aatgtctgcc agccggtttt cgtcggcagg cgctagcggg tccagccctt cgcacatcat
    35761 tcgccatttg accgaccatt cgccgcgctg accattgagt aagcccgtca tctggcgtga
    35821 ccagcttaaa tagtcctgcg ccagcatcgg caggtcgagc aaccattcgc cgggataatc
    35881 cacaatctcc agatacagcg tggaggtgtc cttgaagtgg cgtagcagcg aatcgttcga
    35941 tttaaaacgt agcgccaggc ggatttcact gacgccgcgc gttggcgtcg gccaggcggg
    36001 aggatcgcca tataactgcg ccagtccttc atcatatgtg aagcgcggaa tgccaaagtc
    36061 acgctgagga atgcgtttta cgcccagcag gcgctcttca cgcaccgcgc ttaacagcgg
    36121 caagcgtgct ccggcgtgaa tattgagcaa ctgattgacc atcgcagtga taaacgctgt
    36181 tttgccgctg cggcttagtc cggttacggc gaggcgcaga tgtctgtcga caccccgatt
    36241 caccagcgca ttaagttcat ttttaagtcg cttcattgct gtcctgtgct gctctgaaga
    36301 ccagaaaaaa atcattcgta aggatatgaa tgtgggggcc aggccattga ttatcaactg
    36361 cccgtgttgt catcgacggg cgaactccat aagatgtgca gcattcacgt tcgaacggag
    36421 ttcctgacct atgtccccta ctatttatga tattgccagg gttgcaggcg tatcaaaatc
    36481 caccgtatca cgcgtgctga ataagcaaac taatatctcc ccggaagcgc gcgaaaaagt
    36541 gttacgggcc attgaagaat tacagtatca gccaaacaag ctggcccgcg cgctgacctc
    36601 ttcgggtttt gatgccatta tggtgatttc tacccgttcg accaaaacta cggcgggtaa
    36661 tccgtttttc tctgaagttt tgcatgccat caccgccaaa gctgaagagg aaggtttcga
    36721 cgtgatattg cagacgtcgc acaatccggc agaagactta caaaaatgtg aaagcaaaat
    36781 taagcagaaa atgattaaag gcattattat gctaagttcg ccagcggatg agtcattttt
    36841 tgcccaactt gataaatacg atattcctgt cgtggtgatt ggcaaagttg aaggtcaata
    36901 tgcccatgtt tattctgtcg ataccgataa ttttggcgac agcattgcgt tgaccgatgc
    36961 attaattgaa agtgggcatc aaaatattgc ctgcctgcat gctccgcttg atgtccatgt
    37021 ttcagtggat cgggtaaatg gttataagca gagcctggct gcgcataata ttgcagtgcg
    37081 tgatgaatgg attgttgacg gcggttatac ccatgaaaca gcactgcaag ccgcgcggga
    37141 attattaagc cagtcaccgt tgcctgaggc agtgtttgct actgacagcc tgaaattaat
    37201 gagtatttat cgtgcggcag cagagaaaaa tattgctatt ccgcagcagt tagcggtggt
    37261 gggttatagt aatgaaacgc tgtcgtttat tttaacgcct gcgccaggcg gtattgatat
    37321 cccaacacag gagttaggtc aacaaagctg tgagttatta ttccgcttaa tttctggaaa
    37381 accgtcacca cagaatatta ccgttgccac gcatatgacg ctgaaataaa taccagctac
    37441 cttgcctgaa tgccggatgc gatgctagcg catcttatcc ggcctacatt tatagttcgg
    37501 ataaagtact cgtgccgcat ccggcaattt agcccattat cagaacgagt aatttacgcc
    37561 gacacctgca taatggaatt tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc
    37621 gtattccagc gaaacggaaa gcccgttctg gaaatcataa ccgtaaaata aacctacacg
    37681 gttaaaatca tggccttcac gttcaatatc atcctgccag tcccagttac tccagcgatc
    37741 cagcccaatg cgcgtatacg gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc
    37801 gcgaatttct tgcgtagaaa actcaccgtt attgcggctg tcgtccatat taaagccgcg
    37861 ctcgagataa tagttcactc gcaaggcaac cgtttcgttg aaggtatatt gcaggcctgt
    37921 ttccgtttcg actcgggtat cagcgtaacc ggtcgtgttc agatcgttgg caaatttata
    37981 catcgacaac caaccgttga aacgtaaatc gtcagtcagt ttcacatccc agtctggcgc
    38041 gattttccag cgctgcatat tcgccgtgtc tttacccggt tcatcaacgt agtgataacc
    38101 ataattacgg aaaccgccgg tcaggccgaa actgaaatca tcgttttcga ggaactgata
    38161 atgcacctcc agctccgggc gatcaaacca cgttccacgt ttacccgcgc tataatctac
    38221 cggcccttcc tgataatagg ccagagcaat tctccacggc ccgttggcgg cattaaaata
    38281 gactgaaggc tccgccagcc catccatatc ttcgccataa ccctcgacgt tttctatttc
    38341 gtacatcgcg ccgatattaa agtgccagtc gttcctttcc tcggcctgtg cgcaggccat
    38401 tcccgcacac atcaccagtg cggtacaggg taataacttt ttcattattg ttccttgtat
    38461 tattctgggg gaatgccttc cccctgtatt tagcgaattg ctatttccgt ttctgcatca
    38521 aagaaatgac atttcgtcat atcaaaatga atagtgatat tttctcctgc atgataatca
    38581 tttaacgcac cagcacggac cactaactcg tgccccccaa ccgtggtgta gagcataaat
    38641 tccgcaccgg ttaattctgc cacgctaatt ttggcggaaa tgttattttc ctcttgcgcg
    38701 tccggatgaa tatcttccgg acgtattccc atcacgatgg gcttatgcaa acttccctgt
    38761 gttttcagaa ccgctaattt ctcttcggga atggttaatt taagcgtttc cgtaacgaat
    38821 ttatcgccat cgatcgtgcc acgaataaaa ttcatcgccg gtgatccaat aaatccggca
    38881 acaaacatat tcgctggttg gttataaacc gttttcggcg caccaacttg ctgaacaatc
    38941 ccatccttca taatcacaat ccgcgtcgcc atggtcatcg cttcggtctg atcgtgggtc
    39001 acgtagatca tggtggtatt cagcttctgg tgcagcttgc tgatctctgc gcgcatttgc
    39061 acgcgcagct tggcatcaag gttagagagc ggttcatcca ttaaaaacac gcccgcttcg
    39121 cgcacaatcg cccgcccgag cgccacacgc tggcgttgcc cgccggaaag cgcgcccggc
    39181 ttgcgtttca ggtactcacg caggccgaga atttgcgccg cccagttcac ccgctcatca
    39241 atcacctctt tggcgatttt ttgcatcttc aggccaaacg ccatgttgtc gtaaaccgtc
    39301 atatgcggat acaacgcgta gttctggaac accatcgcta tattgcgtgc tttggctgga
    39361 acgtcattca ttcgtttgcc gtcgatcaac agatcgccgc cgctgatctc ctcaagcccg
    39421 gcaatcatgc gcagggtggt cgacttaccg cagcccgacg ggccgacaaa cacgatgaac
    39481 tctttatcgg caatttccag gttgaagtcc ttcaccacat gcacctggtt atcgtagatt
    39541 ttttgaatat gttgtaacga aagctgagcc atgttgattc ctttgctata cgttttgcca
    39601 gaaggccgat aaccgcgacc aggtcagaga gtccgttgaa ggcaacagta attgcgcccc
    39661 ggttaagccc gcgccgatcc ccaccgagcg cataccgctg gcgttaatgg cgtcaatgcc
    39721 cgcctgcgca tcttcaatgc cgatacatgc ctgcggcggc acgcccagcc cggcacaggc
    39781 agcgagaaag atttccggat ccggtttcga gtttttaagc tgggaagcat ccgcgcagaa
    39841 ggtgaaaaac tcgcgcagct ccagcgccgc taaaatcgtc ggcgcattca gggagacgga
    39901 agccagccct accggaatct gctgtgcacg gagctctgcc agcaaattgc gaataccggg
    39961 cagcacagca ttaaccgtca gctcgcgcag tgagtggacg taaagcagat ttttgcgata
    40021 cgccagttgc gcccgctcct gcgggttaaa gtcgccctct ttgcccccgt gttgcagaat
    40081 gcgccgcaga gactcatcgc ggctaatccc ttttagggac tcgttaaact gcgcatcaat
    40141 gctgatgcca atttcagcgg caatctgctg ccacgcctgg aaatgcagat gcgcggtatc
    40201 ggtgattaca ccatccagat cgaaaattac cccttgcagt ttcattcatc ctcctgatgc
    40261 gtggtagcgg tcctattgaa gggcaaaata taatcaccca aacagaaaac agattcttca
    40321 gccacggata taagctgacc gttcaaacgc agtgaaacgg gcgcagaagt tcgaatcgca
    40381 atacgctgcg cgtcgagagt gacctgtaat tcgcagccct gccagaacag agggaaagac
    40441 aactgttgcc actgctcagg taacgccgga ttgagatgca attcaccgtc atgcacgctc
    40501 accccggcaa aaccctgaat cgcccccagc cagatagcgc cagttgcggc tgcgtggata
    40561 ccgtcatcac agctatgcgg atcagcacca agatcgattt cagtcccctc gcgccagaac
    40621 tgataacttt gcgccaacag gccacagcgt gcggcaacaa tgccgtggat tgctttactt
    40681 aatgacgagt cgtgaatagt gcgcggctca taaaattgca gattggcaag acacgatgcc
    40741 gctgagaact gctctggcag catgtaattg agcatcacca catcagcttg tttgaggatc
    40801 tgcatctcgt tcacttctgc gcgtgaataa tccagcagaa tggtttgctt ccccgccgcc
    40861 gctttgtatt tcgccagatt aatcgccggc ttagtcataa acgaatcatc ctgcggcaaa
    40921 acaccgtcgg gctgaatttc tggcagccgc agttctttaa ggaacatttc ggcacgatga
    40981 ataaacgcgt cgtcactaca gccaaactgg cgggcaatac tcagcgcctg ctggacgttg
    41041 taatacacca tatagctggt gaaggcgtta ttattgacat gttcggtata ttcgtctggc
    41101 ccaataacat catgaatttc cagacggtcg ttaaccctca ccgcgcggct aatccagaac
    41161 tttgcagttt ccataagtag cgccatgcct tcatgagcaa tgaaactttc atcccccgtg
    41221 gtctgccagt attgaataac cgcccaggcg atatcggcca caagatgatg ttccgcctgc
    41281 gccgaggcca ctttttgccg cagcccggtg cgaatgttaa tggcggcaaa ttccggcgtc
    41341 tcttcttcgc cgctgcgcgc gctttcccac ggaaacagag cgccctgcca gccgttgcgt
    41401 cgcgctttct cctgcgcgcc tggcaagttg tgccagcgat accgcagtaa acttcgggca
    41461 accgtcggat cgctaaacag atggaacggc agcaaaaata cttctgtatc ccagaaaacg
    41521 tggcctttgt agccttcgcc ggttaagcct tttgccgcaa tactgctgcg ctcgtcgtga
    41581 gcaggcgtca tgatgcgcag atgataaagc gcataatcta acgcttgctg atcgtgcgct
    41641 tcgccgccat ttaccgtgat acgacgtttc tgccaccatt gacgccagtt ttctgttgat
    41701 actgcaagaa gttggtcgta actctgctgc gcgcacattt caagctggcg aagcgacgcg
    41761 ctgccccact cgtctaaaac ggcttgcctg tcatcccgcc agtcgatcca caccagtttt
    41821 tgcaacgtca ctgtctcgcc tgcatgaagc tgcgcactgg tatgttgctg caaacggcgc
    41881 tctttggcgg tatagcattg ctgcacatca ccgctcacca tacagcaaca gctgatggcc
    41941 acatcactgc gtccatcctg ggtggtgtag atcccctgca tcagatgctg accaaacacc
    42001 cgcacctggg tttcgtcgag atgttggcga ccgtggttgg tttgcgtggc gtcgatacct
    42061 gttgaaatca gcactgacgc gtcggcgtcc agtggcgtaa tagtgatttc cagcacaatg
    42121 agcggcagtt ggtctgccga aacaaagcga cggctggcga tggtgtaacc tgtgccgttg
    42181 ctggtacgcc agacaacgct acggcgtaat tctccactgg caaagtcaag ttcacgctgc
    42241 caggcttcgc gggataacga gaaaacctca ccatttatgg caatctccat ccccaagata
    42301 tcaggcaggt tcaccagttc gttgatttca ccttttcccg cccgatgata cagccccgcc
    42361 agatacatcc ctcgcgtctg gcgcgtgtaa tcttcttcat ggctggcgcg aagcccaaga
    42421 tagccgttcc cctgcgccat cagcgatgca tacttgttca gggtatgctg gctgaaatgg
    42481 ggttctgata acgttactgg cctggtcata attccacacg cgtccctgtt tctgcggatt
    42541 gatacaacgc cgccaccagt tgctggatga tgtacccctg ctcggcgtcg gctatcatca
    42601 cgggcttgcc ctgtacgtga ttgataaagg cttccatgct gcgcaaatgg cggttgtcgt
    42661 ctgccagttc ccgttgcatc agcgtcatta attcaccgtt gttatcggtg tagatatgtg
    42721 ctggaaacag cgtcgcaccg gctttatcac cacaaaagct gacgttcata atcgactgtt
    42781 cgcggatgtt gagtgcaaat gacgtttcca gccacagaat gccgccgtta tgaaattcaa
    42841 tggtgccaaa cagcgaatct tcgacgctat aagttgccgg atcccactcg ccaaattgac
    42901 cacagctctt ttgcgtgccg atcttttgaa agctatgcgc attcacgctt ttcaccgccg
    42961 gaaagcccag cacatacatc gcagcatcca gcatatgaat gccgatgtcg atcagcgggc
    43021 caccaccctg cagttcttta ttggtaaaga cgccccagcc gggaacgccg cagcgacgca
    43081 gggcgcgggc ggtggtaacg taaatctctc ccaaaacgcc gttggtcacc tgttcacgca
    43141 gctgttgcgt atcgagcgca aaacgatggt gaaagtcgta ggccagcacc ttgcccagtt
    43201 tgcgcgcggt atcgcacatt tcccgcgcct gttctggcgt catggcgggc ggtttttcgc
    43261 acatcacatg gcagcccgct tccagtgcca tcagggtatg ttcgtaatga aaacggttag
    43321 gtgagcagac gctaaccaca tcaggtttca ccgccagcag catggcctgc ggatcgtccc
    43381 acacggatgc attaccgtat ttatctgcca gcgcctgcgc ctgggaaagg cggctgtcac
    43441 agacagccac cagttccaga tcgttgcggg tgcagtagta cgaagcatga accttatccg
    43501 ccacctggcc tgcgcctatt atcgcgaccc gcagcggaga gcttgtcatt gcacttttca
    43561 caaagacctc ttagcaggta cgcaaccagg ccaacgaatc acggtacgcc tgggcaggat
    43621 cttccgcccg gatacgccct tcgtacacta catagccctg atagttatcc gcgcgcagct
    43681 gttcaaacag cgcgtggaaa tccagggtgc cgctgcccgg ctggtagcga tggttatccg
    43741 caatatgcac atgaccgagc aggtcgcggt tgtcatgcag cgcctgcgcc aggttatctt
    43801 cttcgatatt catgtgatag aaatcgccga taatctgtac atgtttaaga tcgttttcga
    43861 cgatgtaacg gcgggcatcg gcgagggtgt tgatcatatg atcctgatag cggtttaacg
    43921 gttcgagata caccacggtt ccggtacgcg cggcgacctg ttccagtacg tgcagggaat
    43981 cactcaccat tttgcggtcg ccgtccaggc tacgcggcga ggtcatcggc ggtaagcgga
    44041 aggtaaacat gccccacgct gccggaacga cgataccttt accccccact tctgccagcg
    44101 cttcgagaat gcgttcgatc tgctttaagc cattaagacg acgctcttcg ataaagtcgc
    44161 caatccaacc gtcatagcca ccgcaggcgg tggtcaccgg taaaccggtt tctttgattg
    44221 ctgctttgac ttcttcgaga ttgttgacca gcagtttgcc gtcaatctca aaaccatcga
    44281 agcccatctc tttgatataa cgaaatttct ccagaatgtt ttccggaaag aatgcctgat
    44341 tctgtgtgcc tattttcatt ttttcattcc tttgaattaa aacgtaacgc ccattttgat
    44401 gctctgttcc ggatgctgat cgacatactg catgtagctt tccgggctct tggcaaaggt
    44461 cactaccggg tcgattaaat cttcgcaatt gagataaccg ttcatcagca gttcccagca
    44521 ggtttcttca atacgcttac ggctccagcg cggataatcc gggttcggtt cgctgcacgc
    44581 gcgggagaaa acaattttgg cgttattgaa atgcgcttcg cgtccgaggt taaaaccttc
    44641 agcaaatggt ttggcaaacg cgacatagga gatggtgcca ccgtaggcca gaccgcggaa
    44701 cgccgattgc agcgcgtcgg catagccgct ggtttcgata atcacatcgg caccctgctt
    44761 gccggtcagc gttttgatct ctttgcctac gtcagtacca atggggttaa ggcagaaatc
    44821 cgcgccgtgg cgacgggcaa tatcacagcg atgggcgata ggatcgacgc caatcacgac
    44881 cgacgcgcca gcgcgtttcg ccagttgaat ggcaatttga ccaatcgcgc caagccctac
    44941 caccacaaca aaatcaccca cgcgcacgtt ggcatcgcgc acgccgctca tggcaaactg
    45001 cgccgggtcg taacaaacgg cgtttttcca cgagctgcct tccggcattt tgcgcagctt
    45061 gtagttattc accgcgttaa tgatgaccgt ctcggagagc gggccgtagc cgcatacgct
    45121 gtcgcccacc gcgtagtcgg taacgtcgct gccgcactcg ataatgtcgc caaccaccat
    45181 gtttccaagc tggaatttgc caaactcaat gccgcgcggc gcatctgccg gacgcggggt
    45241 gaacatctgc cactcgccgt taaagtcttc atcgataaac gggctggcgg cgcggaagtc
    45301 gaccacttcc gttccgtgtt tcggtgcgcc gaaacgcacg cggatcttca cttcattgga
    45361 taaaatcgcc cgatcttcat actcaaccag cgcagcaaca cgcggtgctg tggctactaa
    45421 ctttttcatg taaaactcct tgtaaagaaa ctggccgctt tagcccttca cgccaccggc
    45481 ggtcaaacca ctcttaataa aacgttccga aagcgcatac ataatcacca ccggcaatgc
    45541 ggtcaccagc gaggccgcca tcatgcgtcc ccagatgtaa tctggcgtac tgaacagcgc
    45601 gttcaggccc accggtaagg tgaaattgct ggcgctggag aggaaaatcg acgcaaacag
    45661 atagtcgttc cacgccacca taaagcagta gacgaacacg gatatcagcc cggacatcgc
    45721 caacggcacg gtgatgcgga agataatttg cagacggttg aggccatcca tcatcgccgc
    45781 ttcttcgatc tcatcgggga tggtgtcgaa gtagcttttc agcataaaca cggcggtggg
    45841 cagagtttgc gtcaccatgg tgatgatcag cgccatctcg gtgtcgtaaa tgcccagcgc
    45901 ggtgatgatt ttgaacagcg gcaccaccag caaaatgcca gagaacatat acaccgtgta
    45961 aaagctggca ttgatggtca tccgaccttt aaagcgcagg cgggaaagcg cgtaagcccc
    46021 aagaatgccg aggaataccg ccaccacgga tgaaaccacc gacaccacca gactgttacg
    46081 gaagtagtcg acaaacggga aaatcatcgg gttaaaaatg tcgacgtaat gctccagcgt
    46141 ccactgctgc ggcagcagcg taggatgcag tgagatcgcc tctttcgcgc ccttgaacga
    46201 ggtcatcagc atcacaaaaa atggaaacag cgtgatgatg agaaacagcg ccagcccgca
    46261 gtaaaaaccg atgcgactga gtgtgcgttt atttgttgcc attgaggttc acccgtttcc
    46321 tggtcagcag aatgacagcg aaaataatga tgaagagcac caccgagatc gccgccgctt
    46381 ttcctaaatc attaaaggca aaggcggttt tgtagagata tacaccgaga atatcgacct
    46441 tggtggtcag caaataaaca tccgcgaaca tgtagaacat ccagatggtg cgcagggtca
    46501 ccaccgtcgc caggaccggc ataatcgcgg gcagcgtgac gatacgaaac cgttgccagg
    46561 cattagcgcc atccatctct gccgcttcgt acagcgattt gtcgatggtc tgcaaaatcg
    46621 ccagaaacga gataaaggca tacgggaagt agcgccagat ggcgaacagc accaccagca
    46681 caaagctact gcccggatta tcgaaccaca gtggtgcctg ctcatagaga tgcagaagat
    46741 cgacgccgag gtagttcaca atgccgtagc cgttgttgaa catgtatttc caggcaaaca
    46801 ccagcgaaat ggacggcgtt acgtaggaga ggatcaccag cgaacgcgcg gttttgcgca
    46861 ggcggaactc gcggttgaaa aacatcgcca ctgccagccc gagcacggtg ctgcccgcca
    46921 ccaccagcgc ggtataccag accgtcatcc acagcgagtg ccagaagccg ggatcggaga
    46981 ggatacgcac atagttactc accccaacaa aggttgactc gatgttggga ttgagcggca
    47041 gacgtaaaaa actgatttcg atattcgaca ccattggcca ggccaccaga ccacccagca
    47101 gtaataagct gggcgcgaga agcagcagcg caaagggcat atcggaacga cctgaaaaaa
    47161 gcctgttcat tgcggattcc tgtcctgggt ttagtgctgt tcgatcagtt catccagctt
    47221 tttctggctc gcttgcagcg tgctagggag atcggcttta cccacggtga cgttatgcac
    47281 catggagctc accacgccag aacccgtcac atcacccatg cgggtaaagt ttttatcccc
    47341 tactgcacca aagacctgaa tatttggcag ttcaccgatt agctgattcg gtagttcacc
    47401 cagcgcctta ataacgtcgt tgtctttcca ggtggcggta gtgacaaccg ctttattcac
    47461 cggcagtgca gcacctggcg acatcatcac ccagtcggca atgttgtctg cctgctccat
    47521 aaaggtgaca aatttttctg ctgcttccgt ctcttcggtc ttttgcccgg cggtaatggt
    47581 cagcgaggtc aacatgccgt agaccgcaga gtttttctcg gttggcacca cgaaaccgac
    47641 gtttttcggg tcgccttctt taatcacagc cggaaggata taggtggagt aaatcgccat
    47701 cggcgcggtg ccgttcataa aggcgtcttt cacttccatg atgtcgttag aacccggcat
    47761 agtgttggca gcaaggtcgc ggtaataggt cagtgcctgc atcatctctg gtgtatcaag
    47821 ggtgattttg ccttcggcgt taaagacgtt agcctggttg gataacgcaa actgggagaa
    47881 ggattgttcc gtcaacacgc tttctgctgt aggcagcgca atgccgtatt ttttattcgc
    47941 cgggtcattc agtttctgtg caacgtccag cagctgttgc cagtttttcg gctcctcaag
    48001 ccccgctttt gccagcacat ctttgcgata ccagataccg ccaatccagg cgctgacagg
    48061 aacaccggtc catgcgctac catcttcggt acgcacaata cgcagtacgc catcgtaaaa
    48121 cgcgccttca ccaacgttgc tgatgactgt ggcaaccgct ttgcgatcga taagctgctc
    48181 tttgtccatc actttggtgt agtcatggct ggtttctatc acttccggca gcgaaccgct
    48241 acgtgaaaga gtaatgactt tagtgttata ggcatcttct tccacgggca cttgcttaac
    48301 ggtgatgcca gggttttctt tttcaaaacg ggcaatcaat ttactgataa cggcctggcg
    48361 ctcctgctcc accgaagaat gcataaattc gatggataca ttcgttttat tttcttcttt
    48421 acagcctgaa atcagggcgc aggaaaccag tgctgataac agcacgattt ttgatttaat
    48481 catgagatgt tcctttttat ttaatccaca taacctgcca ggggcgcagc gttatttcat
    48541 tacccaatat atcaacttca ctaatcagat cccgaccatg cagattagta atatttacat
    48601 gctgaatatt tttactgaca ttaaacaatc cagtcagaca attaccatca gcgttacttc
    48661 tttgaatacg cattacggat gaattaatcg tatcgatggt aaaattatta tccggatgaa
    48721 actcgttatg gctgcgacga agtgtaatta aacgcgacaa ctcatgatat accgcatgcc
    48781 ttaatgtagc ttcatcgttc agttctcggg ttatctcttt actgtgatat tttttacggt
    48841 taatcgcacg gttatatccg agtttttcga cacctgcgta atcattacgc gagccaagaa
    48901 tactttgaat atatatcgct ggcacaccgg ggaaacttaa caaaatcgca tgggcgagaa
    48961 taaacctggc gcaacgttct tcatcgctac tctcacggcg gcttaacgca tccatatagg
    49021 tcacatttat ttcatacgga ctgcgtgtac cgtcgggatt atttttccag tttactaatg
    49081 caccttcctg ctgtaacgcc tcgaccagct ctaatatttc gctttcaggc aacaagcccc
    49141 gtagcgggtt tagcccgatg ccatcgtgag aggcgaggaa gttaaaccag gtggtgttgc
    49201 tggaaggtag tgtcaggttt tgcgcccacg cacaaagcgc ctcaacgttc tgtttttgca
    49261 ccgcatgcag caccagtggc ggcagcgaga actggtacac catatgtgct tcgtcatcgc
    49321 ctgcgccaaa gtaagcaatg ttgtctttat gcggaacatt ggtctcggta atgatcactg
    49381 tacctggcgc aacgttatca ataatcgacc gtaacagttt gataatcaga tgtgtttttt
    49441 ccagatggat gcagcttgtt cccggctctt tccacataaa gccaacggca tccaggcgga
    49501 catactcagc acctttcgca aggtaacaga gtaaaacatc caccatcgcc agcaacactt
    49561 cagggctacg gtaattcagg tcaatttgat cgtcactaaa ggtggtccat aaatggcgcg
    49621 ttgaatgatc gcgcatctgg aatggcgtta ataacggtaa cgcacgcgga cgggtgacgg
    49681 cgctgagatc ggtttgcgga tcaaccgcaa taaaaaaatc ttcaaaacct ggctgctgtt
    49741 gtaaatagtt tttaaaccat tcactttttg ccgacatatg gttgcagaca aaatcaaaca
    49801 ttaaatgact gcattcaccg agttgctgaa tatcctgcca ctcccccgct tcactggcga
    49861 cctgatgata atcaattacc gaaaagccat catcagatga ccatggataa aacggcaata
    49921 aatgaacatg tgaaaaaatg ctttgcagcc attgatggta aaactgatta aatgtgggta
    49981 atggttttaa atcattgctg tgaaattgat cggcataggt aattaacacg acatcacttt
    50041 catcccagtg ttttttacgt cgctgtgtaa ttaatcgttt cgcactctca agacgcgtta
    50101 caagtttctg taaatgagtt gcggtaaatg ttccaccgta gatttcgtcc aggtaatccg
    50161 taattttctg tttcatgttg cctttccgtg gtagcggtcc catgaaacta gagactactc
    50221 cgcagaaaat aaactgtcaa ttgagcacaa gggttttagt cacgcaggaa atcatttttt
    50281 accagggaaa aagcgtattt gtgagctgac gcaaataatc tatggggatg taaataaggt
    50341 gtgacagaaa aaaaacggcg cataagcgcc gctcatggtg aattctttta acctttgacc
    50401 ttcggcattg cgatgtcttt caggccaccg gcgttctcaa cgtgggtata tcccatctcg
    50461 ctaaggatct cttttgcttg ccctgactgg cgtccggcat tgcaatacac tttcacggtg
    50521 tcgtttttat ccggaacggc ggtggcaatg cgctctttca cttccttcag gggaatattg
    50581 atggccccct gaacgtgctc ttgctgatac tgctctggaa cacgaacatc gatccagtgt
    50641 tcagcggcga aaacgggcag tgaaaacacc agtgccagag caagtaagcc ttttttaaac
    50701 atgtaagcgt ccttctatgg atgaaaattg ccatccatta tacgcctgag atttaacaat
    50761 ttttgcgaaa ctcccctcac cttttataac gctgtgccag tttattagca gcccgactta
    50821 acagcggttc cagcgccaca gccagcaaca ttttcagcgg tcggcgagca actgatttta
    50881 tcgcccagcc cgctacaccc gccgggccat agcgcagtgc ggtaagaagt accagcttgc
    50941 ctgctaattt gaaaccaggc tttacctttt gcccggcctg ttgccagcga gtattcataa
    51001 ctttcctcac agttgacgga aacggctacg taacgtgaaa gtatcggaag tgacataacg
    51061 ttccatctcg cgtaaacgcg tttcacttgc cgccagttca cggtcgactt catccagcaa
    51121 ttcgctgctg gaaggtagct gctcaccaaa ggccatgttg tccggcattg gatcaagcgc
    51181 aaatgacaaa atgatgtaag caaccagggt aaacagcgcc agaccgaaga aaatcgacag
    51241 caccaccagg atacgcacca gttttaccgg tacatcaaaa tagttggcaa tcccggcgca
    51301 gacgccgcgg accatgccct gctgtggaat acgccataat tttttattga gattaatgcc
    51361 cgccattagc gatccctcca gttcggatgt tctgcatcaa gaatagattc cagcgcctga
    51421 atacgttcgc gcatccgttt tgcttcatca gccagttgcg ctaatcgctg ctgctcactt
    51481 tgcgacaatt cactgcgacc agaacgattg ctgtaatgca gccataacca gatcggtaaa
    51541 acaaacagca caaaaatggt taacggaata gccagaaata gcgcgctcat aagtactcct
    51601 tagacgatga gcggcggcgc gttcggacgc cgcctggatg ttattgttga ttgtcttgct
    51661 tcattttggc ttttaattgt gccagttgtt cgctgattgc atcatcggct ttcagttcgg
    51721 caaactgatc gtccagcgat ttttgtttac cgaagctgtg gctttctgct tccgcttcca
    51781 tctggtcaat acgacgttcg aaagattcga aacgagccat tgcttcatcc agtttgccac
    51841 tgtccagctg acgacgcaca tcgcgcgacg agtttgccgc ctgatgacgt aacatcaatg
    51901 cctgctggcg agcgcgtgtt tcgctcaatt tgttttccag ctcgccaatc tctttcttca
    51961 tgcgtgccag cgtatcgtcc accagcgtca cttcatgttc cagggactta atcagatcgg
    52021 tcagtttctg tttttcaatt aacgctgcac gtgccagatc ctctctctct ttcagcagcg
    52081 ccagttcggc tttttcctgc cattcaactt cacgcgccga cgcttgttca atacggcgag
    52141 tcagctgttt cttttctgcc aacgcgcgcg ccgaagtaga acgtacttca accagtgtat
    52201 cttccatctc ctggatcatc agacgaacca gtttctgtgg atcttccgct ttctctaaca
    52261 gagcgttgat gttggcattc acgatgtcgg caaagcgaga aaaaataccc ataatgttgt
    52321 cctcttgatt tctgcgttca ggattgtcct gctgaactgt taatacaatt tgcgtgccaa
    52381 ttttttatct ttttgattta taaagatctg attgaagaat caacagcaac atgccaggat
    52441 gagttagcga attacactaa caagtggcga atttcatcat ggcagaatac aaagataatt
    52501 tacttggtga ggcgaacagc tttctcgaag tgctggaaca ggtttcgcat ctcgcaccgc
    52561 tggacaaacc ggtgctcatc atcggcgaac gcggcaccgg taaagagctg attgccagcc
    52621 gcctgcatta tctctcctcc cgttggcaag ggccgtttat ttcccttaac tgcgcggcgt
    52681 taaatgaaaa tctgctggat tccgaactgt ttggtcacga agcgggggcg tttaccggtg
    52741 cgcaaaaacg tcatccaggg agatttgaac gtgccgacgg cggtacgcta tttcttgatg
    52801 aactcgctac ggcaccgatg atggtgcagg agaaattatt gcgtgtgatt gagtacggtg
    52861 aactggagcg cgttggcggc agccagccat tgcaggtgaa tgtgcggttg gtatgcgcga
    52921 cgaatgccga tctcccggcg atggtcaatg aaggcacttt tcgcgctgac ctgctcgacc
    52981 gactggcttt tgatgttgta caactgccac cactgcgcga gcgcgaaagc gacataatgt
    53041 tgatggcaga acactttgcc atccagatgt gtcgggaaat caagctgcct ctgttcccgg
    53101 ggtttacgga gcgcgccaga gaaacattgc tgaattatcg ttggccggga aatattcgtg
    53161 aattgaaaaa cgtggtggaa cgttcagtgt atcgccacgg caccagcgat tatccgcttg
    53221 atgacatcat tattgatccc tttaaacggc gtccgcctga agacgctatc gccgtttcag
    53281 aaaccacctc gcttccaaca ctgccgctgg atttacgtga gtttcagatg cagcaggaaa
    53341 aagagttgct gcaactcagt ttgcaacaag ggaaatataa ccagaagcgc gcggctgaat
    53401 tactggggtt aacttatcat cagttccgcg cgttgttgaa aaagcaccag atttagcgaa
    53461 gttgagcaat acaacttgcc ggatgcggcg tgaatgcttt attcggcata cataatcaat
    53521 acgtcgtttg caggcctgat aagcgcagcg catcagacat tattgcgttg ggctattaat
    53581 cgctcagcgc atcctgcaaa attttcattg ccgcatcgaa ttgcgcatcc gggatggtca
    53641 gcggatacag gaagcgaatc acgttgccgt atgcgccaca ggtcagcagg agcagcccct
    53701 gcgccagcgc gcgttgctgg attttctgtg caatcgccgc tgacggctcg cccgtttgcg
    53761 gatcgttaaa ctctgccgca atcatcgacc ccaggccgcg taccgcagca atggccggaa
    53821 cgctttcttt ggcatcaatc aacgtgtttt tgagacgctg gcccagttga ttcgcgcgtt
    53881 cgcagagtga ttctttgtcg ataatattga gcaccgcgtg cgcggcagcc accgccagcg
    53941 ggttcccggc gtaggtgcca cccaacccgc ccggcgcggg cgcgtccata atattcgcgt
    54001 taccgaccac gcccgaaagc ggcatcccgc ccgcgaggct tttcgccatc gtcattaaat
    54061 ccggcttatc gacgtaatga tccatggcaa acagtttacc ggtacgcgca aagccgcttt
    54121 gcacttcatc ggcaatcatc acaatgccgt gctcgtcgca caggcggcga atggcggcaa
    54181 ccagctcttt tggcgcaacg ttgaaaccgc cctcgccctg caccggttcg aaaataatcg
    54241 ccgccacctg cttcgcttcg atgtctgatt taaacaagcg ttcgatggcg tcgagggagt
    54301 cctgtgttga aatgccgtgt aaatctgacg gataaggtac gtgatacacc gaaccaggga
    54361 acgggccgaa gccgattttg tacggcgcga cctttccagt taacgccata gtcatatacg
    54421 tacgaccgtg gaagccgccg ctaaacgcaa tcacgccagg gcgtccggta tgggcgcgag
    54481 caattttcac cgcgttttcc accgcttccg caccggtagt gaagaacgcg gttttggcct
    54541 gcccgctcac cggggcaagg gcgttgattt tctccgccag ggtgacgtag ctttcatacg
    54601 gcacaatctg atacgcggtg tgggtaaact gttgcagttg ctgctccacc gccgcgacca
    54661 actccgggtg gcgatgcccg gtattcagca ccgcaatgcc tgcggcgaaa tcgatgtatt
    54721 cgttgccctc aacatccttc agcgtggcgt tttcagccga ctgggcgaag aagttacaca
    54781 tcacgccaac cccgcgtggg gtagcagaaa gacgacgctg atggaattca ttgttgctca
    54841 tgtttcacac cttttgactc ttcactgtta atcggaaatt aaacgcccag acgatcgcgc
    54901 aggctgtaat aggctgcgcc catcgcggta aacggtactc gcagcgtgcg cccgccgggg
    54961 aacgggtaat gcggcagatt ggcgaaggca tcgaaacgtt cggcgtcgcc gcgcagcagt
    55021 tcggcaatca gacgcccggc aagatgggta caggtgacgc cgtggccgct gtagccctgc
    55081 atgtaataga tgttggtatc gaggcgaccg aactgcggca tccgtgaaag ggtcagcagg
    55141 aagttgcccg tccagcggta gtcgattttg acgcccttca gttgcgggaa ggttttcagc
    55201 agtttcggca ccacaagacg ctcaacgtca tccgggtcgc gcgcgccgta gaccacgccg
    55261 ccgccgtaca gcaaacggtt gtcggcggta agacggtagt aatcaagcag ataattacag
    55321 tcttccacac agtagttttt cgggatcagc gaacgggcta aatcttccga cagccgttcg
    55381 gtggtgatca cctgcgtgcc gcacggcatg ctgcgtttcg ccagttccgg ctccacttta
    55441 tcgcccaggt acgcattccc ggcgacaatc acatacttcg ccgtcacctg acctttggca
    55501 gtacgcacca cggcgggcgt ggtgtgctgg atctgcgtga ccgcagaaag ctcatacacg
    55561 cgcccgccgt tcaggcggat ggcatcggct tcaccaatgg cgaggtttag cgggtgaata
    55621 tgcccaccgc tgtgatccag cagcgcaccc gtgtagcgat cgctggctac ttcacggcga
    55681 atggcgttag cgtccagcag ctccagctgt ttattgccgt agcgttccca gttctctttc
    55741 tgctcttcga gtgtggcgag ctgtttatca ttcatcgcca caaacaggcc gccggggcgg
    55801 tagtcgcagt caatctgata acgtttgata cgttcgcgga tgatctcgcc accctcgaac
    55861 atcatgctgc cgagcatacg ggcggtgtcc atgccgtagc ttttttcgat cacgtcgatg
    55921 tcgcggctgt aggagttcac aagctgcccg ccattgcgcc cgcttgcgcc aaagccgatg
    55981 cgtgaggctt cgagaaccac tacgtcaaag cctgcttccg ccagatgcag cgcggaggag
    56041 agcccggtat agccgccgcc aaccacgcaa acgtcgcagg tgattgactc attcagtgtg
    56101 tcgaatggcg cgtatttatt cgcactggcg gcgtagtaac tactggtatg ttcggtcatt
    56161 tcaggcctcc aggcttatcc agatggtttt cagttcagtg aatttttcaa gggcatgcag
    56221 ggatttgtcg cgaccgttgc cgctctgctt atagccgcca aacggcacgg tcatatcgcc
    56281 gtcgttgtag ttattgacga agacggaacc ggctttcagg cggcggctca tacggtgcgc
    56341 gcgggagagg tcgcgcgtcc ataccgccgc gccaaggccg tactggctgt cgttggcaag
    56401 ctgtagcgcc tggtcttctg atgtgaaacg cgtgaccacc agcaccggac cgaaaatctc
    56461 ttcgcggctt aaggacgcat tcgggtccac atccacaaag atggtcgggc cgatggcggc
    56521 agccagctcg gcattacggc catccagcag cagttgccct ttgctttcgc cttcccgaat
    56581 aaagctatgg accgagtcgg cgtgggcgca gtcgattaag gtgcccatgg tggttgcggg
    56641 atcaagtgga tggcccggct gccagttttg cgcctgctgt tttaacaggg ctaagaattc
    56701 atcggcgatg ctctcttcca gtaacaaacg cgttccggcg atgcacacct gaccctggtt
    56761 gtagaagatg cctgctgcgg tggagctcgc cgctttttgc agatccggac aatcggcgaa
    56821 aacgatgttg gcgcttttgc cgcccgcttc cagccagacg cgtttcatgt tgctgtcgcc
    56881 cgcatctttc agcagctgtt tcccggtacg ggttgaaccg gtaaaggcaa tggcgtcgat
    56941 atcgttatga cgcgacagcg cctgcccggc ttcatgacca aaacccgtca ccacgtttaa
    57001 cacaccatcc ggcaagcctg cttctttcgc cagccccgcg agacgaatcg cactgagcgg
    57061 tgatttttca gacggtttta gaatcacgct gtttcccgcc gccagcgccg ggccgagttt
    57121 ccagcaagtc agcaacagcg ggaagttcca cggcacgatg gcggcaatca cgccgaccgg
    57181 ttcacgcacg atcatcgcca gctcatggct actggtggtc gccacttcgc catacacttt
    57241 gtcgatcgct tcggcgtacc agcgaatggc gcgcgccgcg ccgggaatat catcacgcag
    57301 actgtgacga atcggtttgc cggtgtcgag agtttccagc agtgccagct cttcggcgtg
    57361 ggcttccatt aaatcggcga gtttattcag taccgcttta cgtttagccg gagaagagag
    57421 tgaccagtcg ccgcgttcaa atacgccgcg tgctgcgctc atcgcacggt cgatatcgac
    57481 gctcttgccg cgggcaattt tcgccagcgg tgcctgggtg accggatcaa cggtttcaaa
    57541 ggtttcattt tccgccgcag cagtatattc accgttaata aataagcggt tttcaatggc
    57601 gagacttaac gctttatcct gccagtaagc cagatgatga aaattcatta tgactcctgt
    57661 ttcacgtcta tcagatatat gcagtttgtg aacgtcaggg cgcagagatc cccgggccgt
    57721 atgcccgttt atttctggcg acattcttta tatacacttc atccttcaag ccgcctctgc
    57781 cttggctgcg ttcgctcccc cgaatcactt acctgagtaa gctcatcggg attcgctcac
    57841 ttgccgcctt gatgcaactt gaatgatttt gtgtatagat tgccgggcgc attgcgctta
    57901 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
    57961 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
    58021 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
    58081 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
    58141 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
    58201 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
    58261 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
    58321 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
    58381 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
    58441 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
    58501 tagtggtcag agtcgctgtt tttcagcgat atggtgctga caagcggtga taaagccctc
    58561 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
    58621 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
    58681 ttcaacacgc aaccgtgggc taacgacctt cgccccttgc ccatgcagag agtttaccca
    58741 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
    58801 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
    58861 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcctg
    58921 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattaacgc
    58981 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
    59041 gtgcggctgc acattgctgg gactaccagg aagataaatg ccatcgagtt tcggcaaaag
    59101 ttgttcaagt aatgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
    59161 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    59221 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    59281 cacaaagtgg actaaattat cgccactact gcggtttaaa tgacctttat gttcaatatt
    59341 ttttcaatct agcagtgggt tttcattttt gcaaactcaa tttaacatct gacaaacatt
    59401 tagtttgcat acagattcga atggtggtca ttatatttta cgctttgata acgagcggaa
    59461 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    59521 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    59581 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    59641 aaaacgtatc cctgtttcaa gcctgaagaa gttggagaaa gggtgttatt tcccggcctc
    59701 ggtgtttgca atggatattc tgggcaacgt agtagaagaa gcgggtctgg gtcaggaaat
    59761 gggcgagccg gatcgtactt gtgttcctgt tctcggttcc ttaacccctt ctgccgccga
    59821 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    59881 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    59941 cccggtcgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    60001 gtatctgcaa ccgccctgcg cgcccggcac cgatgaccgc aatacacaaa gccaagttta
    60061 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg agctggcgca
    60121 gttacagtta attcccgccg acggtgcggt cgccgaagcc tcgccgggtc agtttgaaat
    60181 caacctttac cataccgata acgtgctgga agcctgcgat gatgcgctgg cgctaaaacg
    60241 cctggtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    60301 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    60361 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    60421 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    60481 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    60541 cgtcgccctg cgtattccgt gcggcgaccg tcataatcac cgcgtggaat atcgcgtggc
    60601 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    60661 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    60721 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    60781 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    60841 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    60901 attccccatg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    60961 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    61021 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    61081 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    61141 taaacaggtt tgatttcagg ttgacacgac cgcaaacctt attacgcagg ggaggcagca
    61201 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    61261 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    61321 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    61381 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    61441 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaatcc gcacgtcgga
    61501 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    61561 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    61621 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    61681 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    61741 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    61801 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    61861 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    61921 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    61981 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    62041 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    62101 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    62161 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    62221 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    62281 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    62341 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    62401 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    62461 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    62521 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    62581 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    62641 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    62701 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    62761 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    62821 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    62881 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    62941 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    63001 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    63061 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    63121 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    63181 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    63241 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    63301 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    63361 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    63421 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    63481 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    63541 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    63601 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    63661 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    63721 cttcgacagt ctgcaatttg ccgataacgt gaaaagcgtc cgtaaactgg ataatcatac
    63781 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    63841 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    63901 actcgaccgt caaccggtcg gcaccggtcc gtatcaattg tcggaatacc gcgccgggca
    63961 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    64021 ggtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ctggtgaatg
    64081 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    64141 gcgtttaacg ctgcgtcccg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    64201 gccgctaaat aatcccgctg tccgccatgc gctggcactg gcgattaata accagcgcct
    64261 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    64321 gtgggcctat gacaacgagg ctaaaattac tgaatacgat ccggcgaaat cgcgcgaaca
    64381 gttgaagtcg ttggggctgg aaaatttaac gctgaaactg tgggtgccca cacgttcgca
    64441 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    64501 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    64561 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    64621 cttccgtcct ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    64681 cgatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    64741 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattgccca ttttgccgct
    64801 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    64861 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaagcc
    64921 atgattatct tcaccttacg ccgcattttg ttattgattg ttaccttgtt cctgctgacc
    64981 ttcgttggct tcagtttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    65041 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    65101 attaacggtc aaccaattgc cgaacagtta aaagaggtct tcccggcgac gatggaactg
    65161 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    65221 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    65281 atcccggtgt tctggctggc gcttctgttg acgctgtttt gttcactcac gctaggttgg
    65341 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    65401 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    65461 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    65521 ctgatgcgta tcagcactat cgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    65581 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    65641 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    65701 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    65761 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    65821 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    65881 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    65941 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcggggctgg ctgtactgtg
    66001 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    66061 attgccgccg tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    66121 cggacgcgat gttttaagcc gcttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    66181 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    66241 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    66301 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    66361 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    66421 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    66481 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    66541 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    66601 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    66661 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    66721 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    66781 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    66841 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    66901 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    66961 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    67021 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    67081 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    67141 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    67201 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    67261 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgacc
    67321 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    67381 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    67441 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    67501 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    67561 agcgcgatgc cgcataaaag tcgcctcaat acgctgcccg gcgctatccc actgctggaa
    67621 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    67681 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    67741 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    67801 ccgctaccgg accggctggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    67861 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    67921 gctggcaaaa atgctggcgg gaatgataga gcctaccagc ggcgagttat tgattgacga
    67981 tcatccactg cattttggcg attattcctt ccgcagtcag cgtattcgca tgatttttca
    68041 ggatccttcg acctcgttga atccccgtca acgtatctcg caaattctgg attttccact
    68101 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    68161 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    68221 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    68281 tgaagccctc gcttcactgg atatgtcgat gcgttcgcag ttgattaatc tgatgctgga
    68341 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    68401 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    68461 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    68521 tggcgaagca ttgacggcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    68581 ccagattatc cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    68641 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    68701 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    68761 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    68821 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    68881 gctggaccgt gctgcgtttc tggcataacg agattgattg taatgaggag gcggtgctgg
    68941 agattattct gcaggaactg aaccgccgat caccctcacc ctgaaagggc gagggggccg
    69001 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    69061 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    69121 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    69181 taaggggaaa agcgcccacc ctgccgcagc ctgctccggt cggacctggc aactatagct
    69241 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    69301 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    69361 ctttccggta agcgcattct ggtaaccggt gttgccagca aactatccat cgcctacggt
    69421 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    69481 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    69541 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    69601 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    69661 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    69721 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    69781 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    69841 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    69901 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    69961 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtaccgt tactattgaa
    70021 gatgtgggta actccgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    70081 gtggtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga attgaaataa
    70141 tcattctgtt ggtaaatatt gggcggcgtt ctgccgcccg ttatctctgt tatacctttc
    70201 tgatatttgt tatcgccgat ccgtctttct ccccttcccg ccttgcgtca ggataacgat
    70261 ttcctttacg accaaggagc gcccatggaa caacgccaca tcaccggcaa aagccactgg
    70321 tatcatgaaa cgcaatccag tactgcggag tatgacgttc tgcctctggt cccggaagcc
    70381 gcaaaggtca gcgatccctt tctgctcgac gtgatccttg atgaagaaac gctggctccc
    70441 ttcctttcat ggctggtccc tgcgcgcgtt cttgcagtgg aattgttccc tgaccagctt
    70501 accgtgaccc gttcacagac cttcaccgct tatgaacgct tgtctacggc cctgacggtt
    70561 gctcaggttt gcggcgtcca gcggttatgt aactactatt cggcgcgact tacgccgctc
    70621 cccgggcctg attcctccag ggaaagcaat cataggttgg cacaaatcac gcaatatgcc
    70681 cgccaactgg ctagctcgcc ttctattatc gacaaccgat cgcgccagca tctgaatgac
    70741 gtcggtctta ctgcctggga ctgtgtgatc attaaccaaa tcattggttt tattggcttt
    70801 taggcgcgga ccattgcgac atttcaggct tatctcgggc acccggtacg ctggttaccc
    70861 ggtctggaaa tacaaaacta cgccgacgcg tcactgtttg ctgatgaatc aatacgctgg
    70921 cgaagcagct atgaggtgga aaaactacct gaagagtaca caaaaagttc aactgcagaa
    70981 ctttgccaac tggccgaaac actctctctc caccctattt cactttccct tctcgaaagg
    71041 ttgttaaaca gtacacgggt taatacacag ccggataata agcttgcggc gttgttatgc
    71101 gcgcggataa atggcagtcc tgcttgtttt gccgcctgta tggattcatc aaatgaatat
    71161 aaaaaaatca gcgcccttgt gcgcaagggc gaaaatgaaa ttaaccaatg ggctgaccgt
    71221 cattctgttg agcacgctac cgttcaggcg atacaatggc tgacccgagc acccgatcgc
    71281 tttagcgccg cccagttcag ccctttactc gaacacgaaa aatcatcaac gcagattatt
    71341 aatctgctgg tatggagcgg gctgtgtggc tggataaatc gcttaaaaat cgcgttgggt
    71401 gagacatatt aaccttgccg cgtcagacag attcgcgtaa aactgtcagc cgctctaatg
    71461 gccaccaaaa tagacaatta tgtttcagga caacccgctg ctagcgcagc ttaaacagca
    71521 actgcattcc cagacgccac gcgctgaagg ggtggtaaaa gccacagaaa aaggctttgg
    71581 cttcctggaa gtcgacgcgc aaaaaagtta tttcattccg ccgccgcaga tgaaaaaagt
    71641 catgcatggc gaccgaatta tcgcggtgat ccacagtgaa aaagaacgtg aatccgcaga
    71701 gccagaagaa ctggttgaac cgttcctgac tcgtttcgtg ggtaaagttc agggcaaaaa
    71761 tgaccgtctg gccatcgttc ctgatcatcc actcttaaaa gacgccattc cttgccgcgc
    71821 agcccgtggc ctgaaccacg agtttaaaga aggcgactgg gcggttgccg aaatgcgccg
    71881 tcatccgctg aaaggcgatc gttctttcta tgctgaactg acacaataca tcacttttgg
    71941 tgacgaccac tttgtaccgt ggtgggttac ccttgcgcgc cataatctgg aaaaagaagc
    72001 accagacggc gtcgctaccg aaatgctcga tgaaggtctg gttcgtgaag atctgaccgc
    72061 gctggatttt gtcaccatcg acagtgccag cacagaagat atggatgacg cccttttcgc
    72121 taaggcgttg ccggatgaca aacttcagct gattgtggcg attgccgatc caaccgcgtg
    72181 gattgctgaa ggcagcaagc tggacaaagc cgcgaaaatt cgcgcattca ccaactatct
    72241 gcctggcttc aacatcccta tgctgcctcg cgagctttct gacgatctct gctcactgcg
    72301 cgccaatgaa gtccgcccgg tactggcatg ccgcatgacg ctctccgctg atggcaccat
    72361 tgaagataat atcgaattct ttgccgccac catcgaatcc aaagcgaagc tggtgtatga
    72421 ccaggtttct gactggctgg aaaataccgg tgactggcag cctgaaagtg aagcaattgc
    72481 cgaacaagtc cgtttgttag cgcaaatttg ccaacgccgc ggcgagtggc gtcataacca
    72541 cgcactggtg tttaaagatc gcccggatta ccgctttatt ctcggtgaaa aaggtgaagt
    72601 gctggatatc gtcgccgagc ctcgtcgcat tgccaaccgt atcgtcgaag aagcgatgat
    72661 tgccgctaac atttgtgcgg cccgcgtact gcgcgataag ctcggttttg gcatctataa
    72721 cgtgcatatg ggctttgatc cggcgaatgc cgacgcgctg gcagcgttgc tgaaaacgca
    72781 cggtctgcat gtcgatgccg aagaagtgct cacgctggac ggtttctgca aactgcgtcg
    72841 tgaactggat gcgcaaccaa ctggtttcct cgacagccgc attcgtcgct tccagtcatt
    72901 tgctgaaatt agcactgaac ccggtcctca ctttggcctc ggtctggaag catacgccac
    72961 ctggacttcg ccgatccgta aatatggcga catgatcaac caccgtctgc tgaaagcggt
    73021 tatcaaaggc gaaactgcga cgcgcccaca ggatgaaatc actgtccaaa tggccgagcg
    73081 tcgccgtctc aaccggatgg cagaacgtga tgttggcgac tggttatacg cacgcttcct
    73141 gaaagacaaa gccgggaccg acacccgttt cgcggcggaa attgtcgata tcagccgtgg
    73201 cggcatgcgt gtgcgtttgg ttgataacgg cgctatcgcc tttattccgg cacctttctt
    73261 acacgctatg cgcgatgaac tggtttgcag ccaggaaaac ggcactgtac aaattaaagg
    73321 tgaaacggtt tacaaagtaa ctgacgttat tgacgtcacc attgccgaag tccgcatgga
    73381 aacccgcagc attattgcgc gcccggtcgc gtaatctcct ttcacggccc attcctcatg
    73441 gatgggccgt ttatttcccc gctctacctt cgttatatcc aggcagattt tttaactgac
    73501 tttcatttga aaactggcgt ttttccaaaa ctggattaca aataaatata taaaaaaaac
    73561 aatgcgttta gccgcagtta atttcatgta agtcatgttg ttccttttat ttaaccgcgg
    73621 actccgctgt taaccggagg atatgcatca tgaaaaccgt tagggagtcc acaacgttgt
    73681 acaactttct cggatcgcac aatccatact ggcggttgac ggaaagcagc gatgttttgc
    73741 gcttttctac caccgaaacc acagaacctg atcgtacatt gcagttatct gccgaacagg
    73801 ctgctcgcat cagggaaatg acggtcatca cctccagcct gatgatgagt ctgaccgtcg
    73861 atgaaagcga tctttctgtg catctggtag gacgaaaaat caataaacgg gaatgggctg
    73921 gcaacgcgtc tgcatggcat gacacaccgg cggttgctcg tgatttatca cacgggcttt
    73981 cctttgctga gcaggtagtt tctgaagcac attccgcaat agtgattctc gacagccggg
    74041 ggaatatcca acgcttcaat cggttatgtg aagattacac agggttgaaa gaacacgacg
    74101 tcattgggca aagcgtgttt aaactgttta tgagccgtcg tgaagctgcg gcatccaggc
    74161 gcaataaccg tgtatttttt cgaagcggca atgcatatga agtcgaactg tggataccaa
    74221 catgtaaagg ccagcggctg tttctgtttc gcaataaatt tgtccacagc ggcagtggca
    74281 aaaacgagat ttttttaatc tgttccggca ccgacattac cgaagagcgc cgcgctcagg
    74341 agcgactgcg tattctggca aataccgaca gtatcaccgg actgccgaat cgtaacgcaa
    74401 tgcaggattt aatcgatcac gctattaatc atgcagataa caataaagtt ggggttgtgt
    74461 atcttgattt ggataatttc aaaaaggtca acgacgccta tgggcatttg tttggtgacc
    74521 agttattacg cgacgtgtca ttggctattt taagctgtct cgaacatgac caggtgttgg
    74581 cgcgtccagg tggcgatgag tttctggtac tggcatccaa tacctcacaa agcgcgctgg
    74641 aagcaatggc atcacgaatt ttgacccgct tacgcctccc ctttcgtatt ggtttaattg
    74701 aagtttatac cagctgttca gtaggtattt cgctctctcc cgaacatggt tcggacagcg
    74761 cgactattat tcgtcacgcc gacacagcaa tgtacacagc gaaggaaggc ggacgaggac
    74821 aattttgcgt ttttactcca gaaatgaatc agcgggtatt tgaatatctc tggctggata
    74881 ccaacttgcg taaagcactg gaaaacgatc agttggttat tcactatcaa ccgaaaatca
    74941 cctggcgtgg cgaagtgcgc agtctggaag cactagtacg ttggcagtca cctgaacgtg
    75001 ggttgattcc accgttggac ttcatttcct acgccgaaga gtcagggcta attgtgcctt
    75061 taggccgttg ggtgattctc gatgtcgtac gccaggtggc aaagtggcgg gataaaggca
    75121 taaacctgcg agtggcggta aatatttctg cacgtcagct cgccgatcaa accattttca
    75181 ccgccctgaa acaggttctc caggaactca attttgaata ctgccctata gatgttgaac
    75241 tgacagagag ttgtctgatt gagaatgatg aactggcact gtctgttatt caacaattta
    75301 gccaactagg tgcgcaagtg catctggatg attttggtac cggctactct tcactttcgc
    75361 aactggcgct ctttccgatc gatgccatca aacttgacca ggtttttgtt cgagatattc
    75421 acaaacaacc tgtctcgcag tcactggtcc gggcgatcgt cgctgtggcc caggcattga
    75481 atcttcaggt gatcgccgaa ggtgtagaga gtgcaaagga agatgctttt ttaaccaaga
    75541 acgggatcaa tgagcggcaa ggatttttgt ttgccaaacc gatgcccgcc gtcgccttcg
    75601 aacgctggta taaacgctat ctgaagcgcg cataagtaga tgaatatata tacttagcgc
    75661 tatatcccgt cattatatat cgtattcctg gatgataatt agccgttgct gttaaacact
    75721 tctcttttag cggcaatttt tgcattattc attttccact tttactataa ggatgtcctt
    75781 gatgtctgaa tttgatgccc agcgcgttgc cgaacgtatt gatattgtgt tagatatcct
    75841 tgtggcaggc gattaccact ctgcgatcca taatctcgag atacttaaag cagaactctt
    75901 gcgccaggtt gctgaatcta cgccagacat cccgaaggca ccgtgggaga tttaacaatc
    75961 gtgccttcag gttttcctat ttgaggatgc ccggcgcgtc agtatttttt tatttagtat
    76021 tataacgtta taagaattac agcgatgaat tcccgacaac aaactattct acagatggtc
    76081 attgaccagg gtcaggttag cgtaaccgat ctggcaaaag ccactggagt ttctgaagtt
    76141 accattcgcc aggatctcaa caccctcgaa aaactgagtt acctccgccg tgcacatggc
    76201 tttgcagttt cgcttgatag tgatgacgtc gaaacccgta tgatgagcaa ctatacgctg
    76261 aagcgtgaac tcgccgagtt tgccgcgtct ctggttcaac cgggcgaaac catctttatc
    76321 gaaaatggta gcagcaatgc cctgctcgcc cggactctgg gcgaacagaa gaaaaatgtc
    76381 actatcatca cagtcagcag ctacattgcg catttgctga aagacgcgcc ttgtgaagtc
    76441 attttgctcg gtggcgtgta ccagaaaaaa agcgaaagta tggttggccc tttgacacgc
    76501 cagtgcatcc agcaggtaca tttcagcaaa gcatttattg gtattgatgg ctggcaacct
    76561 gaaactggat tcaccggtcg cgatatgatg cgtaccgatg tggtcaatgc cgtgctggaa
    76621 aaagagtgcg aagcgatagt cctgactgac agctcgaaat ttggtgctgt acattcatac
    76681 tccatcggtc ccgttgagcg attcaatcgc gtgattaccg attcgaaaat acgcgccagc
    76741 gatctgatgc atcttgagca cagtaaactc accgttcacg tcgttgacat ttaaccattt
    76801 cccttctcta cggatgattt gcagtttggc aaatcatccg ctctaagatg attcctggtt
    76861 gataattaag actatttacc tgttattaac acgcttaaga tataaaatta ttatcagcga
    76921 tataacagga agtcattatc acctgcgtga tataaccctg cgcgcgagca gatttcacgg
    76981 aataatttca ccaggcttat tcttagctat tatagttata gagagcttac ttccgtgaat
    77041 cataaattca ggagagagta ttatgtttgt aacgagcaaa aaaatgaccg cggctgttct
    77101 ggcaattact ttggcaatgt ctctgagtgc ctgttctaac tggtctaaac gggaccgcaa
    77161 caccgcaatc ggcgcgggtg caggagcatt aggcggtgca gtactgaccg atggcagtac
    77221 gttgggtaca ttaggtggtg cagctgtcgg tggtgttatt ggtcaccagg tcggtaaata
    77281 atcgtaaata aatgtcgatt atcgactcgc aattataata cgcttgattc tgttcagtac
    77341 gaaataactg aataatacgc acagtaaaat attcgaaagc cacggatata tccgtggctt
    77401 tttatgttaa ccgcctgcga gttttacctt catccctttc gcttcaagta gtgattttaa
    77461 taaatcacgc ttatcgccct ggatttcaat aactccatct ttaactgctc cgccgcagcc
    77521 gcattttttc ttcagttccg ctgcaagttt tgtcagttcg gcatcatcga gatcgacacc
    77581 ggtaatcagg caaacgccct tacctttacg tccactggtc tgacgctgaa tacgcaccac
    77641 accgtcgcct ttagggcgta caggggccgc tttgggctca tcaatacgtc cggtttccgt
    77701 tgagtagacc agacggctgt tggaatcact catcatgcac tccgctgtaa agaggcgttg
    77761 atcgctttca gcgtctgcgc tggatctacc gattgcgtta ccgggcgacc aatcaccata
    77821 taatcaacac cagccgacaa cgcctgttct ggcgtcataa tgcggcgctg gtcaccagct
    77881 tcactcccct gcggacgaat gcccggcgta accagtttga actcctgacc gaatacctgt
    77941 ttgaaacgta cagcttcctg agcagaacac accacaccat caaggccaca tttttgcgtc
    78001 agtgctgcca gacgttctgc ataatctgca ggtgacagtg taacgccaag atcggccagg
    78061 tcgctagctt ccatgctggt caacactgtc acagcaatca aaagcggtgc atcattgcca
    78121 aacggaacca gtgcctcacg cgctgcggtc atcatacgcg ccccaccaga ggcatgaaca
    78181 ttcaccatcc acacgcctaa gtcagctgca gcagcgacag cgtgcgctgc agtgttgggg
    78241 atatcgtgga atttcatgtc aagaaagata tcaaaaccac gctgttgaag ttcgcgcaca
    78301 aactgtggcc caaacaatgt aaacatctct ttgccgacct tcagacgaca atcgcgtggg
    78361 tcgatcttgt cgacaaaggc cagcgcggca tcacgattat gataatcaag ggcaacaacc
    78421 acaggagaat tcgtaacagc gcgggaagaa gatgaagcag ttaacgtcat gaccagacct
    78481 tcttgatgat gggcaccgga agtggcgcga aacaggtaaa cggcgagcat tctacccgtg
    78541 gaaataaaaa attaacagtt gcgatttctc ccctggcagg ctttttctgc tccgtcgttg
    78601 ccggagcaca atggaacatc aatatctagt atgttgtaac taaagtcgga tttttttaaa
    78661 aattacaggc catcaagacc gcgaatcggt ttaatggttg accaggcccg acaagacgga
    78721 caatgccagt agagggtgtc tgcggtaaaa ccacatttct ggcagcgata acgaggctta
    78781 ctacgtacct tttcgccaac catgtcacgc agcaccatca ggctctcttt ggcacgccct
    78841 tcttccgctt catttaagtg gtaatccatt aacttatgga acacacgcat ggtcggatga
    78901 cgctgaagct ggcgcgtaat atagatctgt gcagcctcac taccgtcgcg cgcttcgatg
    78961 atatccgcaa gcatcaattc agcatcggca ccggtgttct cttccaccgc gcgttgcagg
    79021 aattctgccc attcggcagt tttacccaac tgctgatagc aggtttgcaa catttccagc
    79081 gtttcgctga ccagttctct gtcctgggat atcacgcgtt gcaaactttc gacggctttg
    79141 gcgtattctc ctttcgccat aaacacgcgt cccatcatga tggatacgcg ggcgctgttt
    79201 ttatctgccg ccgccccttt ttttagcaag gtcatggcac gatcgagatc gtcgctggcc
    79261 atatgctgca gggctaactc acagtagaaa tgggcaattt cgacgcgctg tttatcttta
    79321 cccagcttca ccaggcgttc ggcaacatca attgctttct gccactcact ggtagcctgg
    79381 tagatttgta gcaactgttg cagcgcgcca atgcggaagt cagtttcatc ggtcagctga
    79441 ttgaacatgt cttccgcgcg gtcgtataac ccggcggcca tgtaatcacg ccctagttgt
    79501 tgaatcgcca acagacgctg ttcataggtc agcgaggcgc tttccattag ggtctgatgg
    79561 atgcgaatag cgcgatcaac ttcgccacgc gaacggaaca ggtttccgag cgtaaggtgg
    79621 gcttcaacgg tgcctgtatc ctctttaagc atatcgagaa acaggtctac cgctttatcc
    79681 tgttgattac taagcaggaa gttaaccccc gctacgtaat cacgcgacaa gcggttggct
    79741 tcatcttgct tgttttgttg cgcacttctg cggcccatat accagccata ggcagcggct
    79801 acaggcaaaa gcagaaacaa caactccagc atagaaagtt attccttcgc cgctgacgag
    79861 tgcggcacta cagccacgtc agtcgcgggt gaaagctggt tttccagtcg ctttatttta
    79921 cgttcagcgc gcgccaggga aacacgaact cgcagccaga acaggccaca aatcaaccaa
    79981 ccgatagcaa accccgcagc aaacaatacc gccagcaatg tggaaatacg gtactcccct
    80041 tgcgctaaca gataattaaa cgtcacctgt tgatcgttct gcgcacccaa cgtgaccgaa
    80101 atcacgaaga tcgctaacac cagtaagaaa atgagtaaat atttcacatt acttcccgtt
    80161 atgtggttag agcgaataaa ttatggtcaa cttaccgcaa tagcccttta aagtaccatt
    80221 ttcgcgccgt ctgcgaaatg gcatccacgc gcattgttga tgatttaagg gctaaattcg
    80281 gaaaatcaac cagcgttaac tttcttgttc tcgttgcgct atttcgcgat tttcttccgc
    80341 aggtggtgtt aatggcccac aaattcgttg cgcaagccac gttgccaccg ccaccagcgc
    80401 ccacgaaatc aacgtagcta ctaccagatc gcgtggccaa tgcatcccga gcagcaggcg
    80461 gcttcccatg actcccgttg cccagaccag caagatagca atggttaacg ttcgccgacg
    80521 cggccacagc aaaccaacgg ccagcagtgc ccaactggca gcaaacatcg tgtgaccgga
    80581 aggaaaggca aaccccgtct ctttctgcca gtgtgaacgc aaatattgtg ggatattttt
    80641 ctcttcagcc aactgttctt tcactaaatt tccgcgttct gctcgcttta aagtgtagaa
    80701 ctcatcaacc ggaatatgat gtgttttttc cagccagata acaaaaggtc gtggttcctg
    80761 gactttgtct ttgatccagg atttaacgcc ttgtcccaca aggattgcgg ccgccagaat
    80821 ggcaaataat acaaaggcag ccttaatgcg aaaacgcaga caccagagaa accagccgaa
    80881 taaaatcaaa tgtgtaatga cgccccaggg ctgggtgaca gtttcagtaa cccaaaaagc
    80941 cgcttttagt agccaacttt gttctccagg ttgccaacgc cagccagaaa tccatacggc
    81001 tactggcatg acaagcaata gtgcagctcc cactgcggta cgtctggcaa tcgaacgcat
    81061 ggcctctcct ttttgataag tcccacaatc ataactgaaa acgccagttc caggaaaaat
    81121 tgacagattt gtgccattcc gtgaacgatc gacgcgtcgt gattaggtga accccttctc
    81181 gttatggcaa aataagccaa tacagaacca gcattatctg gagaatttca tgcagcttaa
    81241 acgtgtggca gaagccaaac tgccaacccc atggggcgat ttcctgatgg tgggatttga
    81301 agaactggca accggacacg atcatgtcgc gctagtctat ggcgatattt ccgggcatac
    81361 cccggtactt gcgcgcgtcc attccgaatg tctgaccggt gacgccctgt tcagcttgcg
    81421 ctgcgattgt ggcttccagc tcgaagcggc attgacgcaa attgccgagg aaggccgtgg
    81481 tattttgctg tatcaccgtc aggaaggtcg taacattggt ctgctgaata aaatccgcgc
    81541 ttacgcactg caggatcaag gttacgatac cgtagaggct aaccaccagt taggcttcgc
    81601 cgctgatgag cgcgacttca ctctttgcgc tgatatgttc aaactccttg gcgtcaatga
    81661 agtccgcttg ttaaccaata acccgaaaaa agtcgaaatt ctgaccgaag cagggattaa
    81721 tattgttgaa cgcgtaccat tgattgtagg tcgtaacccc aataacgaac attatctcga
    81781 taccaaagcc gagaaaatgg gccatttgct gaacaaataa ccctcttgca ttgtgtaatt
    81841 agtttgcttg ccggaagcaa aataaccggc aagcaaatac ttgttacttc agcatattac
    81901 gaatgacata atgcaaaatg ccgtcgttct ggtagtaggt caactccgtc gcggtgtcga
    81961 tacgacaacg gcagggtacg acctcctggc taccatccgc acgcgtaagc gtcaccggaa
    82021 ccgtcgcacc gggttgtaga ttttgcagat cgccaatatc aatcttctct tccccggtta
    82081 gtcctaacgt tttacgcgtt acgccttgcg gaaattccag cggcaggatg cccatgccaa
    82141 ttaaattcga acggtgaatt cgttcaaacg attcggcaat caccacacga attccaagca
    82201 gacgaggacc ttttgccgcc cagtcacggc ttgagcctga accatactct ttcccggcaa
    82261 tcaccgccag cggcgtttgt tcctgcttat agcgcatcgc agcatcataa atagagacta
    82321 cgtcgctgtc aggtaaatgc cgcgtcatcc ccccttcaac gccaggcacc atttcattac
    82381 ggatgcgaat attggcgaag gtgccgcgca tcatcacttc atggttacca cgccgcgaac
    82441 cgtaggagtt aaagtctttt cgctcaacac cccgaccttg tagatatcga cccgctgggc
    82501 tgtcgggctt aatactgccc gccggagaga tatggtcagt ggtgactgaa tcccccagca
    82561 ttgcgaggat ccgcgcaccg tgaatatctt ccactggtgc tggtgttgcc tgcatttcat
    82621 caaagaaagg cgataagcga atataggttg agtcctcctg ccaaccgtag gtatcggatc
    82681 gtgtgacgtt aatttccttc cactctgctg tgccttcaaa aacttctgcg tactctttgc
    82741 ggaacatttc tgtggagact tgttctaccg cacgggcaat ttcttgtgcc gatggccaga
    82801 tatctttcag ataaaccgga tcgcctttac gatcatgacc aataggctca gaagccaggt
    82861 tgatattcat atttcccgcc agcgcatagg caaccaccag cggcggcgag gccagccaat
    82921 tggttttaac cagcggatgg atacggcctt caaagttacg gttgccggac agcactgcac
    82981 cgacggttaa atcgcctttt ttgattgccg tttcgatagg atcgggcagc ggcccagagt
    83041 taccaataca ggtagtacaa ccgtatccca caaggttaaa ccccagttcg tcgagaaacg
    83101 atgtcagttt cgcttttgcc agatagtcag aaacaacttt cgagcctggt gccagcgacg
    83161 ctttgaccca cggttgccgc ttgaggccaa gaattacggc ttttttcgcc agcaagcctg
    83221 cagccatcag cacacttggg ttagaggtgt tggtgcacga ggttatcgca gcaatgacca
    83281 cagcgccatc aggtaactga tactgatgtc cgttcataac ataatcgacc ggctggcgat
    83341 ctttatgcgt ggcattcact tccagttcgt tactggcggc aaatgctttt ggtacatcgg
    83401 gcagtgcaac gcgatcctgt gggcgttttg gccctgccag gctcgcttca acgtcattca
    83461 tatccagttc taacatactg gtaaaaatcg gttcatcgcc cgggttacgc cacatgccct
    83521 gcgctttggc atatttttcg accaactcaa cctgatcttc gctgcgcccg cttaaacgca
    83581 tgtaatcgag ggttacagca tcgattggga agaagccaca ggtggcacca tattctggcg
    83641 acatattggc aatggtggcg cgatccgcca gcggtagtga atccagacca tcaccataaa
    83701 attcgacgaa tttccccacc acgccatgtt tgcgcagcat ttgggtaaca gtgagaacca
    83761 ggtctgtggc ggtaatacct tcacgtaatt taccggtaag tttaaagccc actacgtccg
    83821 ggataagcat ggaaaccgg
//
LOCUS       DAZKIV010000003        79250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZKIV010000003 DAZKIV010000000
VERSION     DAZKIV010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 79250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 79250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..79250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.003"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..649
                     /gene="yraH"
                     /locus_tag="ACHOT6_000192"
     CDS             65..649
                     /gene="yraH"
                     /locus_tag="ACHOT6_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9945484.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            729..1424
                     /gene="yraI"
                     /locus_tag="ACHOT6_000193"
     CDS             729..1424
                     /gene="yraI"
                     /locus_tag="ACHOT6_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9945485.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKIADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1454..3970
                     /gene="yraJ"
                     /locus_tag="ACHOT6_000194"
     CDS             1454..3970
                     /gene="yraJ"
                     /locus_tag="ACHOT6_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9945486.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSNNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGAIASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3981..5072
                     /gene="yraK"
                     /locus_tag="ACHOT6_000195"
     CDS             3981..5072
                     /gene="yraK"
                     /locus_tag="ACHOT6_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9945487.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(5115..5975)
                     /gene="rsmI"
                     /locus_tag="ACHOT6_000196"
     CDS             complement(5115..5975)
                     /gene="rsmI"
                     /locus_tag="ACHOT6_000196"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFP9945488.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            6039..8075
                     /gene="lpoA"
                     /locus_tag="ACHOT6_000197"
     CDS             6039..8075
                     /gene="lpoA"
                     /locus_tag="ACHOT6_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFP9945489.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIYATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVRAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8033..8428
                     /gene="yraN"
                     /locus_tag="ACHOT6_000198"
     CDS             8033..8428
                     /gene="yraN"
                     /locus_tag="ACHOT6_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFP9945490.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDVWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMRESRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8448..9038
                     /gene="diaA"
                     /locus_tag="ACHOT6_000199"
     CDS             8448..9038
                     /gene="diaA"
                     /locus_tag="ACHOT6_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFP9945491.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9048..9623
                     /gene="dolP"
                     /locus_tag="ACHOT6_000200"
     CDS             9048..9623
                     /gene="dolP"
                     /locus_tag="ACHOT6_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFP9945492.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9737..10777)
                     /gene="yraQ"
                     /locus_tag="ACHOT6_000201"
     CDS             complement(9737..10777)
                     /gene="yraQ"
                     /locus_tag="ACHOT6_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9945493.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQVDANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10850..11485)
                     /gene="yraR"
                     /locus_tag="ACHOT6_000202"
     CDS             complement(10850..11485)
                     /gene="yraR"
                     /locus_tag="ACHOT6_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFP9945494.1"
                     /translation="MSQVLITGSTGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPLFYNRVKGEMEEALIAQNWQKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            11613..12131
                     /gene="yhbO"
                     /locus_tag="ACHOT6_000203"
     CDS             11613..12131
                     /gene="yhbO"
                     /locus_tag="ACHOT6_000203"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFP9945495.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12111..12554)
                     /gene="yhbP"
                     /locus_tag="ACHOT6_000204"
     CDS             complement(12111..12554)
                     /gene="yhbP"
                     /locus_tag="ACHOT6_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFP9945496.1"
                     /translation="METLTAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEEIDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12605..12907
                     /gene="yhbQ"
                     /locus_tag="ACHOT6_000205"
     CDS             12605..12907
                     /gene="yhbQ"
                     /locus_tag="ACHOT6_000205"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFP9945497.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGTGFAELLSSLQTPEIKSD"
     gene            complement(12894..13397)
                     /gene="yhbS"
                     /locus_tag="ACHOT6_000206"
     CDS             complement(12894..13397)
                     /gene="yhbS"
                     /locus_tag="ACHOT6_000206"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9945498.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13391..13915)
                     /gene="ubiT"
                     /locus_tag="ACHOT6_000207"
     CDS             complement(13391..13915)
                     /gene="ubiT"
                     /locus_tag="ACHOT6_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFP9945499.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14124..15119
                     /gene="ubiU"
                     /locus_tag="ACHOT6_000208"
     CDS             14124..15119
                     /gene="ubiU"
                     /locus_tag="ACHOT6_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFP9945500.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15128..16006
                     /gene="ubiV"
                     /locus_tag="ACHOT6_000209"
     CDS             15128..16006
                     /gene="ubiV"
                     /locus_tag="ACHOT6_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFP9945501.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16087..17094
                     /gene="yhbW"
                     /locus_tag="ACHOT6_000210"
     CDS             16087..17094
                     /gene="yhbW"
                     /locus_tag="ACHOT6_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HFP9945502.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17212..18456)
                     /gene="mtr"
                     /locus_tag="ACHOT6_000211"
     CDS             complement(17212..18456)
                     /gene="mtr"
                     /locus_tag="ACHOT6_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFP9945503.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18610..20499)
                     /gene="deaD"
                     /locus_tag="ACHOT6_000212"
     CDS             complement(18610..20499)
                     /gene="deaD"
                     /locus_tag="ACHOT6_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFP9945504.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20492..20572)
                     /gene="yrbN"
                     /locus_tag="ACHOT6_000213"
     CDS             complement(20492..20572)
                     /gene="yrbN"
                     /locus_tag="ACHOT6_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFP9945505.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20679..21563)
                     /gene="nlpI"
                     /locus_tag="ACHOT6_000214"
     CDS             complement(20679..21563)
                     /gene="nlpI"
                     /locus_tag="ACHOT6_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFP9945506.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21672..23807)
                     /gene="pnp"
                     /locus_tag="ACHOT6_000215"
     CDS             complement(21672..23807)
                     /gene="pnp"
                     /locus_tag="ACHOT6_000215"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFP9945507.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24054..24323)
                     /gene="rpsO"
                     /locus_tag="ACHOT6_000216"
     CDS             complement(24054..24323)
                     /gene="rpsO"
                     /locus_tag="ACHOT6_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFP9945508.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24472..25416)
                     /gene="truB"
                     /locus_tag="ACHOT6_000217"
     CDS             complement(24472..25416)
                     /gene="truB"
                     /locus_tag="ACHOT6_000217"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFP9945509.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25416..25817)
                     /gene="rbfA"
                     /locus_tag="ACHOT6_000218"
     CDS             complement(25416..25817)
                     /gene="rbfA"
                     /locus_tag="ACHOT6_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFP9945510.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(25981..28653)
                     /gene="infB"
                     /locus_tag="ACHOT6_000219"
     CDS             complement(25981..28653)
                     /gene="infB"
                     /locus_tag="ACHOT6_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFP9945511.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKSEAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28678..30165)
                     /gene="nusA"
                     /locus_tag="ACHOT6_000220"
     CDS             complement(28678..30165)
                     /gene="nusA"
                     /locus_tag="ACHOT6_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFP9945512.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30193..30645)
                     /gene="rimP"
                     /locus_tag="ACHOT6_000221"
     CDS             complement(30193..30645)
                     /gene="rimP"
                     /locus_tag="ACHOT6_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFP9945513.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30852..30928)
                     /locus_tag="ACHOT6_000222"
     tRNA            complement(30852..30928)
                     /locus_tag="ACHOT6_000222"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30892..30894),aa:Met,seq:cat)
     gene            31276..32619
                     /gene="argG"
                     /locus_tag="ACHOT6_000223"
     CDS             31276..32619
                     /gene="argG"
                     /locus_tag="ACHOT6_000223"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFP9945514.1"
                     /translation="MTTILKHLPLGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKVK"
     gene            32803..33756
                     /locus_tag="ACHOT6_000224"
     CDS             32803..33756
                     /locus_tag="ACHOT6_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9945515.1"
                     /translation="MNGEYFVRLALHTLKCTQKDLASHLGVSSTQISKWKKGEHMSAD
                     MEKKFRNITQIGDYSPQLVEWTGSVANAEKWDRLIHFLARRAMDDAETGYITQLLTDE
                     DGFLVEETIDVLKRIGFPTPLSFPEGLNIDDDNADEEEAFWEILESNAHCSVINDIYH
                     ALNDVYGFYIAYVDELIQDDDLDVYSSEAINIQSSLISLAACKIEIDTPVASNFKEFR
                     YRVKKDYENWLNQLKMMAFRAGIPLRAELLEMVYNTADQLSVAAEAERFDFNKSRIHP
                     DIYMNEILTGMRIIHQVLPVIMQKLEITDFKLDETDLCLGK"
     gene            complement(33831..33917)
                     /locus_tag="ACHOT6_000225"
     tRNA            complement(33831..33917)
                     /locus_tag="ACHOT6_000225"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33881..33883),aa:Leu,seq:gag)
     gene            complement(33932..34264)
                     /gene="secG"
                     /locus_tag="ACHOT6_000226"
     CDS             complement(33932..34264)
                     /gene="secG"
                     /locus_tag="ACHOT6_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFP9945516.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(34492..35829)
                     /gene="glmM"
                     /locus_tag="ACHOT6_000227"
     CDS             complement(34492..35829)
                     /gene="glmM"
                     /locus_tag="ACHOT6_000227"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFP9945517.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLGEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(35822..36670)
                     /gene="folP"
                     /locus_tag="ACHOT6_000228"
     CDS             complement(35822..36670)
                     /gene="folP"
                     /locus_tag="ACHOT6_000228"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFP9945518.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(36760..38694)
                     /gene="ftsH"
                     /locus_tag="ACHOT6_000229"
     CDS             complement(36760..38694)
                     /gene="ftsH"
                     /locus_tag="ACHOT6_000229"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFP9945519.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(38794..39423)
                     /gene="rlmE"
                     /locus_tag="ACHOT6_000230"
     CDS             complement(38794..39423)
                     /gene="rlmE"
                     /locus_tag="ACHOT6_000230"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFP9945520.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            39549..39842
                     /gene="yhbY"
                     /locus_tag="ACHOT6_000231"
     CDS             39549..39842
                     /gene="yhbY"
                     /locus_tag="ACHOT6_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFP9945521.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(39998..40474)
                     /gene="greA"
                     /locus_tag="ACHOT6_000232"
     CDS             complement(39998..40474)
                     /gene="greA"
                     /locus_tag="ACHOT6_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFP9945522.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            40722..42155
                     /gene="dacB"
                     /locus_tag="ACHOT6_000233"
     CDS             40722..42155
                     /gene="dacB"
                     /locus_tag="ACHOT6_000233"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9945523.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(42195..43367)
                     /gene="cgtA"
                     /locus_tag="ACHOT6_000234"
     CDS             complement(42195..43367)
                     /gene="cgtA"
                     /locus_tag="ACHOT6_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFP9945524.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRIIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(43383..44348)
                     /gene="yhbE"
                     /locus_tag="ACHOT6_000235"
     CDS             complement(43383..44348)
                     /gene="yhbE"
                     /locus_tag="ACHOT6_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9945525.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVCMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYALCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(44475..44732)
                     /gene="rpmA"
                     /locus_tag="ACHOT6_000236"
     CDS             complement(44475..44732)
                     /gene="rpmA"
                     /locus_tag="ACHOT6_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFP9945526.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(44753..45064)
                     /gene="rplU"
                     /locus_tag="ACHOT6_000237"
     CDS             complement(44753..45064)
                     /gene="rplU"
                     /locus_tag="ACHOT6_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFP9945527.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            45323..46294
                     /gene="ispB"
                     /locus_tag="ACHOT6_000238"
     CDS             45323..46294
                     /gene="ispB"
                     /locus_tag="ACHOT6_000238"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFP9945528.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            46522..46800
                     /gene="sfsB"
                     /locus_tag="ACHOT6_000239"
     CDS             46522..46800
                     /gene="sfsB"
                     /locus_tag="ACHOT6_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFP9945529.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(46848..48107)
                     /gene="murA"
                     /locus_tag="ACHOT6_000240"
     CDS             complement(46848..48107)
                     /gene="murA"
                     /locus_tag="ACHOT6_000240"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFP9945530.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGAAIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(48162..48416)
                     /gene="ibaG"
                     /locus_tag="ACHOT6_000241"
     CDS             complement(48162..48416)
                     /gene="ibaG"
                     /locus_tag="ACHOT6_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFP9945531.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSDDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(48576..48869)
                     /gene="mlaB"
                     /locus_tag="ACHOT6_000242"
     CDS             complement(48576..48869)
                     /gene="mlaB"
                     /locus_tag="ACHOT6_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFP9945532.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(48869..49504)
                     /gene="mlaC"
                     /locus_tag="ACHOT6_000243"
     CDS             complement(48869..49504)
                     /gene="mlaC"
                     /locus_tag="ACHOT6_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFP9945533.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(49523..50074)
                     /gene="mlaD"
                     /locus_tag="ACHOT6_000244"
     CDS             complement(49523..50074)
                     /gene="mlaD"
                     /locus_tag="ACHOT6_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFP9945534.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(50079..50861)
                     /gene="mlaE"
                     /locus_tag="ACHOT6_000245"
     CDS             complement(50079..50861)
                     /gene="mlaE"
                     /locus_tag="ACHOT6_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFP9945535.1"
                     /translation="MLLNVLASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(50869..51678)
                     /gene="mlaF"
                     /locus_tag="ACHOT6_000246"
     CDS             complement(50869..51678)
                     /gene="mlaF"
                     /locus_tag="ACHOT6_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFP9945536.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            51888..52865
                     /gene="yrbG"
                     /locus_tag="ACHOT6_000247"
     CDS             51888..52865
                     /gene="yrbG"
                     /locus_tag="ACHOT6_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFP9945537.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            52879..53865
                     /gene="kdsD"
                     /locus_tag="ACHOT6_000248"
     CDS             52879..53865
                     /gene="kdsD"
                     /locus_tag="ACHOT6_000248"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFP9945538.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            53886..54452
                     /gene="kdsC"
                     /locus_tag="ACHOT6_000249"
     CDS             53886..54452
                     /gene="kdsC"
                     /locus_tag="ACHOT6_000249"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFP9945539.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            54449..55024
                     /gene="lptC"
                     /locus_tag="ACHOT6_000250"
     CDS             54449..55024
                     /gene="lptC"
                     /locus_tag="ACHOT6_000250"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFP9945540.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            54993..55550
                     /gene="lptA"
                     /locus_tag="ACHOT6_000251"
     CDS             54993..55550
                     /gene="lptA"
                     /locus_tag="ACHOT6_000251"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFP9945541.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            55557..56282
                     /gene="lptB"
                     /locus_tag="ACHOT6_000252"
     CDS             55557..56282
                     /gene="lptB"
                     /locus_tag="ACHOT6_000252"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFP9945542.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            56330..57763
                     /gene="rpoN"
                     /locus_tag="ACHOT6_000253"
     CDS             56330..57763
                     /gene="rpoN"
                     /locus_tag="ACHOT6_000253"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFP9945543.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            57786..58073
                     /gene="hpf"
                     /locus_tag="ACHOT6_000254"
     CDS             57786..58073
                     /gene="hpf"
                     /locus_tag="ACHOT6_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFP9945544.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            58191..58682
                     /gene="ptsN"
                     /locus_tag="ACHOT6_000255"
     CDS             58191..58682
                     /gene="ptsN"
                     /locus_tag="ACHOT6_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFP9945545.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            58728..59582
                     /gene="rapZ"
                     /locus_tag="ACHOT6_000256"
     CDS             58728..59582
                     /gene="rapZ"
                     /locus_tag="ACHOT6_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFP9945546.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            59579..59851
                     /gene="npr"
                     /locus_tag="ACHOT6_000257"
     CDS             59579..59851
                     /gene="npr"
                     /locus_tag="ACHOT6_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFP9945547.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            60064..60696
                     /gene="yrbL"
                     /locus_tag="ACHOT6_000258"
     CDS             60064..60696
                     /gene="yrbL"
                     /locus_tag="ACHOT6_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFP9945548.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGNPSITLTEFAEQCR
                     YEEDVAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(60693..61421)
                     /gene="mtgA"
                     /locus_tag="ACHOT6_000259"
     CDS             complement(60693..61421)
                     /gene="mtgA"
                     /locus_tag="ACHOT6_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFP9945549.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSAPSGYVRS
                     RQAWILRQMYQLGGESFMQQHQLD"
     gene            complement(61418..62071)
                     /gene="elbB"
                     /locus_tag="ACHOT6_000260"
     CDS             complement(61418..62071)
                     /gene="elbB"
                     /locus_tag="ACHOT6_000260"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFP9945550.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(62301..64637)
                     /gene="arcB"
                     /locus_tag="ACHOT6_000261"
     CDS             complement(62301..64637)
                     /gene="arcB"
                     /locus_tag="ACHOT6_000261"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFP9945551.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(64733..65662)
                     /gene="yhcC"
                     /locus_tag="ACHOT6_000262"
     CDS             complement(64733..65662)
                     /gene="yhcC"
                     /locus_tag="ACHOT6_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFP9945552.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            66337..70797
                     /gene="gltB"
                     /locus_tag="ACHOT6_000263"
     CDS             66337..70797
                     /gene="gltB"
                     /locus_tag="ACHOT6_000263"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFP9945553.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATIKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTSGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDALAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            70810..72228
                     /gene="gltD"
                     /locus_tag="ACHOT6_000264"
     CDS             70810..72228
                     /gene="gltD"
                     /locus_tag="ACHOT6_000264"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFP9945554.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIIEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TREEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDSKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHSMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            72412..73539
                     /gene="yhcG"
                     /locus_tag="ACHOT6_000265"
     CDS             72412..73539
                     /gene="yhcG"
                     /locus_tag="ACHOT6_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFP9945555.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAILDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(73599..74063)
                     /gene="nanQ"
                     /locus_tag="ACHOT6_000266"
     CDS             complement(73599..74063)
                     /gene="nanQ"
                     /locus_tag="ACHOT6_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFP9945556.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(74060..74935)
                     /gene="nanK"
                     /locus_tag="ACHOT6_000267"
     CDS             complement(74060..74935)
                     /gene="nanK"
                     /locus_tag="ACHOT6_000267"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFP9945557.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(74932..75621)
                     /gene="nanE"
                     /locus_tag="ACHOT6_000268"
     CDS             complement(74932..75621)
                     /gene="nanE"
                     /locus_tag="ACHOT6_000268"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFP9945558.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQSVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(75669..77159)
                     /gene="nanT"
                     /locus_tag="ACHOT6_000269"
     CDS             complement(75669..77159)
                     /gene="nanT"
                     /locus_tag="ACHOT6_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFP9945559.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(77268..78161)
                     /gene="nanA"
                     /locus_tag="ACHOT6_000270"
     CDS             complement(77268..78161)
                     /gene="nanA"
                     /locus_tag="ACHOT6_000270"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFP9945560.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(78283..79074)
                     /gene="nanR"
                     /locus_tag="ACHOT6_000271"
     CDS             complement(78283..79074)
                     /gene="nanR"
                     /locus_tag="ACHOT6_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFP9945561.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19501 a        21378 c        19698 g        18673 t
ORIGIN      
        1 aagatgttga acatatattc acattaaata tgattatgta cttgttacaa ggataaggtt
       61 ataaatgaat aaagttacaa aaacagctat tgtgggctta cttgcgcttt tcgcaggtaa
      121 tgccgctgca accgatggtg aaatagtttt tgatggcgag attttaaaat ccgcttgtga
      181 aatcaatgac tctgataaga aaattgaagt tgctcttggt cactataatg ccgaacagtt
      241 tcgttctgtt ggcgatcgta gcccaaaaat tccattcact attcctttag ttaactgccc
      301 agtgactggg tgggagcacg ataacggcaa cgttgaagcg tctttccgtt tatggctgga
      361 aacgcgggat aatggcaccg tccctaactt ccctaatctg gcaaaagtgg gctcttttgc
      421 tggcacagcg gcaacaggtg tgggtatccg tattgacgat gcggaaagcg gaaaccttat
      481 gccactgaat gctatgggga atgataacac ggtttatcag atcccagcgg actccaatgg
      541 tattgtcaat gttgacctca tcgcttacta cgtatcaacc gttgaagcat cagaaatcac
      601 cccaggggaa gcagacgctg tcgttaacgt aacgctggat taccgttaat acgttacggc
      661 gttatctgac ctttcagata acgccctttt ccttcctctt tctcgttgta tcaggttgaa
      721 aaataactat gtcaaaacga acattcgcgg tgataataac cttgttgtgt agcttctgta
      781 tcggccaggc gcttgcaggg ggtatcgttt tacagcgaac gcgagtgatc tatgatgcca
      841 gccgcaaaga ggctgcgtta cctgtcgcaa acaaaggcgc agaaacgcct tatttactgc
      901 aatcatgggt agataatata gatggtacaa gccgtgcccc atttattata accccaccgc
      961 tatttcgtct tgaagctggc gatgactcat cactgcgaat tattaaaata gctgataacc
     1021 tgcctgaaaa taaagagtcg ctgttctaca ttaatgttcg tgccattcca gcaaagaaaa
     1081 aatcagatga tgttaatgct aacgagttga cgctggtatt taaaacacgg atcaaaatgt
     1141 tttatcgccc cgcacacctg aagggacggg taaacgatgc gtggacatca ctggaattta
     1201 aacgtagtga ccattcactc aatatatata acccaactga atattacgtc gtatttgccg
     1261 gactggcagt cgataaaacc gatctcacaa gcaaaattga atatatcgcg cccggagaac
     1321 ataaacagtt accacttcct gcatctggcg gaaagaacgt gaaatgggct gcaatcaatg
     1381 attatggcgg cagttccggg acagaaactc gtccactgca ataacaaata taaaaaacac
     1441 aggtcatcag ggaatgccac aacgacacca ccagggacat aaacgcacac cgaaacagtt
     1501 ggcgctcatc atcaaacgct gtttgccgat ggtgctcact ggcagcggca tgctttgcac
     1561 taccgctaac gccgaagagt attatttcga ccccattatg ctggaaacta caaaaagtgg
     1621 tatgcaaaca actgatctgt cacgtttttc aaaaaaatac gcacaactac caggaactta
     1681 tcaggttgat atctggctga ataaaaagaa ggtttcacag aaaaaaatta catttaccgc
     1741 caatgcagag caacttctgc agccacagtt tacggtagaa caactacgtg agctgggtat
     1801 taaggtggat gaaatcccgg cgctggctga aaaagatgac gatagcgtga tcaactcgct
     1861 tgaacaaatc attcccggta cagctgctga atttgatttc aatcatcagc gacttaattt
     1921 gagcattccc caaattgcac tgtaccgtga tgcaagaggt tacgtctccc cttctcgttg
     1981 ggacgatggt ataccaacgc tgtttaccaa ctactcgttt acaggttcta ataaccgtta
     2041 ccgccagggc aatcgtagcc aacgacagta cctgaatatg caaaatggtg ctaattttgg
     2101 cccctggcga ttacgtaact attctacgtg gacacgcaac gatcaggcgt caagctggaa
     2161 cactatcagt agttatttac aacgtgatat caaggcgttg aagtctcagt tgcttctggg
     2221 agaaagcgcc accagcggca gtattttttc cagctacacc tttactggcg tgcaactcgc
     2281 ttccgacgat aatatgttgc caaacagcca gcgcggattt gccccaacgg tacgcggtat
     2341 cgcaaacagt agtgcaatcg tgactatcag gcaaaatggt tatgtgatct atcaaagcaa
     2401 cgtgccagca ggtgcctttg aaattaacga tctctacccc tcttccaaca gcggcgattt
     2461 agaagtcacg attgaagaaa gtgacggtac acaacgtcgc tttatccagc cttattcttc
     2521 attacccatg atgcagcgac ctgggcatct aaaatatagc gcgaccgctg gacgctatcg
     2581 cgctgatgca aacagtgata gcaaggaacc cgaatttgct gaagccacgg caatatatgg
     2641 tttgaataat acttttacgc tgtatggcgg cctgctcggt tctgaagatt attatgcgct
     2701 ggggatcggt atcggcggca cacttggcgc actgggcgcg ttgtcgatgg atatcaacag
     2761 agctgacacc caattcgata accagcactc ttttcatggc tatcaatggc gtacgcagta
     2821 catcaaagat atcccggaaa ccaacaccaa tatcgctgtt agctactatc gctataccaa
     2881 cgatggctat tttagttttg atgaagccaa tacccgcaat tgggactata acagtcgcca
     2941 aaaaagtgaa attcaattca acatcagcca gacaatattt gatggggtaa gtctgtatgc
     3001 ctccggttcg cagcaagact attggggcaa taacgataaa aacaggaata tctcagttgg
     3061 ggtttccggc cagcaatggg gaattggtta cagcctgaat tatcaataca gtcgctacac
     3121 ggatcaaaat aatgaccgcg cactctcttt gaatctcagt attccgttag aacgctggtt
     3181 accgcgtagc cgggtttcct atcagatgac cagccagaaa gatcgcccaa cccaacatga
     3241 aatgcgtctt gatggctcac tgctggatga tggtcgcctg agctatagcc tggaacaaag
     3301 tctggatgac gataacaacc ataacagtag cctgaacgcc agttaccgtt caccttatgg
     3361 caccttcagt gccggataca gttacggtaa tgacagcagc caatacaatt acggcgttac
     3421 cggcggcgtg gttatccatc ctcatggcgt gacgctctcg caatatctgg gcaacgcttt
     3481 tgcgcttatc gatgctaatg gggcttctgg cgtgaggata caaaactatc cggggattgc
     3541 tactgatccc tttggctatg cagtggttcc ttatctcacg acttaccagg aaaaccgtct
     3601 ctcggtagat actacgcagc tgcccgataa cgtcgatctt gagcaaacaa cacagtttgt
     3661 ggtgcccaac agaggtgcaa tggtagcggc gcgtttcaac gccaatatcg gttatcgcgt
     3721 acttgttaca gtcagcgatc gcaacggtaa accgttgccc tttggcgcta ttgccagcaa
     3781 cgatgagacg gggcaacaaa gtatcgtcga tgagggcggc atactatatc tctctgggat
     3841 atcgagtaaa tcacaaagct ggactgtacg ctggggaaat caggcagatc aacaatgtca
     3901 gtttgctttt agtacaccgg attcagaacc aacaacctct gtattacaag gcacggcgca
     3961 gtgccattaa ggataaaaaa atgaaaagag cgcctcttat aacaggactt ttgttgatat
     4021 ccacatcctg cgcttatgcc tcctcaggag ggtgtggagc cgacagcact agcggtgcga
     4081 caaattacag cagtgtggtt gatgatgtta cggtgaacca gacagataac gtgacaggac
     4141 gggagtttac ctctgcaacg ctaagtagca ctaactggca atacgcctgt tcctgctctg
     4201 cgggtaaggc agttaaactt gtctatatgg tcagccccgt acttaccacc actggacatc
     4261 agacaggata ttacaaactc aatgacagcc tggatattaa aaccacatta caggcaaacg
     4321 acattccagg actcacaacc gaccaggttg tctctgttaa cacccgattc acacagataa
     4381 aaagcagcac cgtatattct gctgcaaccc aaacgggtgt ttgccagggt gacacgtctc
     4441 gttatggacc cgttaatatt ggtgcaaata ccacctttac cctgtatgtc accaagccat
     4501 ttctcggctc gatgaccatt ccgaaaacgg atattgccgt cattaaaggc gcgtgggtcg
     4561 atggaatggg aagcccgtct acaggtgact tccatgattt agtcaagtta tcgattcagg
     4621 gaaatctcac cgccccacag tcgtgcaaaa ttaatcaggg cgatgttatt aaggttaatt
     4681 ttggattcat caatggtcag aagtttacca cccgcaatgc catgccagac ggttttactc
     4741 cagtagactt tgatatcact tatgactgtg gtgatacttc aaagattaaa aactcgttgc
     4801 aaatgcgcat cgacggtaca actggggtag tagaccagta caacctggtc gccaggcgaa
     4861 gaagttcaga caatgtgccc gatgtcggta ttcgtattga aaatctcggc ggcggagttg
     4921 caaatattcc ttttcagaac ggtatccttc ccgttgatcc ttccgggcat ggcaccgtca
     4981 atatgcgcgc ctggccagtt aatctggtcg gtggtgagct ggaaacagga aaatttcagg
     5041 gcacagccac cattaccgtc atggtgcggt aaaagaagac gttgcgtttt gattaacgca
     5101 acgcttcggc actgttaccc ctgctgctcc agcgcatact tatacagcgc atttttcttc
     5161 acgccatgaa tttctgcgac cagcgccgcc gcttttttca gcggcagttc tgcctgtagc
     5221 agcgccagcg tgcgcagggc atcggcgggt aagtcttctt cctgtgcttt atgaccttcg
     5281 acaatcagca ccatttcgcc tttgcgacgg ttttcatctt cctttaccca cgccagcagt
     5341 tcgccaacgg gcgcgccgtg aatggtttcc caggttttgg tcagctcacg cgccagaacc
     5401 acgtagcggg attcgcctaa taccgcaacg atatcttcca ggctatctaa cagacggtgg
     5461 gtggattcat aaaaaatcag cgtgcgcggc tccgcttcaa tggcttttag cgcatcacgg
     5521 cggccttttg atttggcagg taaaaagcct tcgtaacaga aacggtcaga gggtaaaccc
     5581 gcggcgctta acgcagtgat agcagcacac ggcccgggta gcggcaccac acgaatcccc
     5641 gcttcacggc aggtacgcac cagatggtag ccaggatcgt taattagcgg cgttccggca
     5701 tcggaaacca gcgcaatgtt ttgcccctct tgcagcttcg ccagcagcgt ttcggctttt
     5761 tgttgttcgt tatggtcgtg cagcgcaaac aaccgggcat taatcccaaa atgttgcagc
     5821 aataaaccgg tgtgacgagt atcctcggcg gcaatcagat caacggcctg taatacctct
     5881 aacgctcgct gggtgatatc cgccagattg ccgattggcg tcggtacaat gtaaagctgg
     5941 ccctgagaat tatccgccga ttggtgttgt ttcattgtgt cgtccgtatt gccgatttaa
     6001 tattgagcat tgcgtaaaaa aatatcactg gatacattat ggtaccctca acattttctc
     6061 gtttgaaagc cgcgcgttgt ctgcctgttg ttctggcagc cctgattttc gccggttgtg
     6121 gcacccatac tcccgatcag tccactgctt atatgcaggg cacggcgcag gctgattctg
     6181 ccttttatct tcagcagatg cagcaaagct ctgatgatac caggatcaac tggcaattac
     6241 tcgccattcg tgcactggtg aaagaaggta aaaccgggca ggcggttgag ttgtttaacc
     6301 aactaccgca agaactgaac gattctcagc gtcgcgagaa aacactgctg gcggtagaga
     6361 ttaaactggc gcagaaagat tttgctggcg cgcaaaactt gctggcgaaa atcacacctg
     6421 ccgatttaga acaaaaccag caagcgcgtt actggcaggc aaaaatcgat gccagccagg
     6481 ggcgtccttc cattgattta ctgcgcgcgt taattgctca ggaaccgctg cttggcgcga
     6541 aagaaaaaca gcagaatatt tatgccacct ggcaggcgct ctcctccatg actcaggaac
     6601 aggcgaatac gctggtgatc aacgccgacg aaaatattct gcaaggctgg ctggatctgc
     6661 agcgcgtctg gtttgataac cgtaacgatc ccgatatgat gaaagccggg atcgccgact
     6721 ggcagaaacg ttatccgaac aatccgggcg cgaaaatgct gccaacgcag ttggttaacg
     6781 taaaagcgtt taaaccagcc tcgaccaaca aaatcgccct gctgttgccg ctgaatggcc
     6841 aggcagcggt atttggtcgc actattcagc aaggctttga agcggcgaaa aatatcggca
     6901 ctcagccagt ggcggctcag gtagctgccg cacctgccgc agacgtagca gaacaacctc
     6961 agccgcaaac tgcggatggc gttgccagcc cggcacaagc ctcggttagc gatctgaccg
     7021 gtgatcagcc tgcggcccag ccggtgcctg taagcgcccc ggcgacaagc accgcagcgg
     7081 taagggcacc cgcaaatcca tccgcagagc tgaaaatcta cgatacctca tcacaaccac
     7141 ttagccagat cttaagccag gttcagcagg atggcgcgag tattgtggtt ggtccgttgc
     7201 tgaaaaataa cgttgaagag ttgcttaaga gcaacacccc gctgaacgtg ctggcactga
     7261 accagccgga gaatatcgaa aaccgcgtca atatttgtta cttcgcgctt tcaccggaag
     7321 acgaagcgcg cgatgcagcg cgtcatattc gtgaccaggg taaacaagcg ccgctggtgc
     7381 tgatcccacg cagttcattg ggcgatcgcg tagccaatgc gtttgcgcaa gagtggcaga
     7441 aactgggcgg cggcaccgtt ctgcaacaaa aatttggttc caccagcgaa ttacgcgcgg
     7501 gtgttaacgg cggttctggt atcgctttaa cgggtacccc gattactccc agagcgacaa
     7561 ccgactccgg catgacgacc aacaatccaa cgctgcaaac cacgccaacc gatgaccagt
     7621 tcaccaataa tggcggtcgt gtcgatgcgg tgtacattgt ggcaacgccg ggtgaaatcg
     7681 cttttatcaa accgatgatc gccatgcgta acggtagcca gagcggtgca acgctgtacg
     7741 ccagctcccg cagtgcgcaa gggaccgctg gcccggattt ccgactggag atggaaggct
     7801 tgcagtacag cgaaatcccg atgctggcag gcggtaatct accgttaatg cagcaggcac
     7861 tcagcgcggt gaataacgat tattcactgg ctcgcatgta tgcgatgggc gtcgatgcct
     7921 ggtcgctggc aaatcatttc tcacaaatgc gccaggttca gggttttgaa atcaacggta
     7981 ataccggaag cctgacggct aacccggatt gcgtgattaa caggaagtta tcatggctac
     8041 agtaccaaca aggtcaggta gtccccgcca gttaaccact aaacagaccg gcgatgtgtg
     8101 ggaagcacaa gcgcgtcgct ggctggaagg caaaggactg cggtttatcg ccgctaacgt
     8161 gaacgagcgt ggcggcgaga tcgatctgat aatgcgtgaa agccggacca ccgtttttgt
     8221 cgaggtacgc tatcgccgct ctgcgcttta tggcggcgcg gcagccagtg tgacccgcag
     8281 caaacaacac aaactattac agactgcccg cttgtggctc gcgcgtcata atgggagttt
     8341 tgatactgtg gattgccggt tcgatgtggt agccttcacc gggaatgagg ttgagtggat
     8401 taaggatgcc tttaatgacc actcataatt aaggtttaag gattagcgtg caagaaagaa
     8461 ttaaagcttg cttcactgaa agcattcaaa ctcaaattgc ggcggcagag gcgcttccgg
     8521 atgccatctc ccgtgcagcc atgacgctgg ttcagtctct gctcaatggc aacaaaatcc
     8581 tctgttgtgg taatggaact tccgctgcca atgcacagca ttttgctgcc agcatgatca
     8641 accgtttcga aacggagcgg cccagcttac ctgccattgc actaaatact gataatgttg
     8701 tcttaacggc gattgccaac gatcgcttac atgatgaagt gtatgcaaaa caggtgcggg
     8761 cgctgggtca tgcgggagat gtattgttag ccatttccac ccgtggcaac agccgcgata
     8821 ttgttaaagc agttgaagcc gccgttacgc gtgatatgac cattgtggca ttgaccggct
     8881 atgacggcgg cgaacttgca ggtttgttag ggccacagga tgtggagatc cgcattcctt
     8941 cgcatcgtag tgctcgcatt caggaaatgc atatgctgac ggtaaattgc ctgtgcgatc
     9001 tgatcgataa cacgcttttc cctcaccagg atgattaagg agaatacatg aaggcattat
     9061 cgccaatcgc agtccttatt tccgcgctgc tgttgcaagg ttgtgttgcc gctgccgtag
     9121 tgggtaccgc tgctgtgggt accaaagccg caactgaccc acgcagtgtc ggcacccagg
     9181 tggacgatgg taccctggaa gtgcgcgtga acagcgcatt gtcgaaagac gaacagatta
     9241 agaaagaagc gcgcattaat gtaacggcct atcagggcaa agtgctgctg gttgggcagt
     9301 caccaaatgc tgaactttcg gctcgcgcca aacagattgc tatgggcgta gacggtgcca
     9361 acgaagtgta taacgagatt cgtcagggcc agccgattgg tctgggcgaa gcatctaacg
     9421 atacgtggat caccaccaaa gtgcgttcgc agctcttaac cagcgacctg gtgaaatcgt
     9481 ccaacgtgaa agtgaccacc gaaaacggtg aagtgttcct gatggggctg gtgactgaac
     9541 gtgaagcgaa agcggcggca gatattgcca gccgggtgag cggcgtaaag cgggtaacca
     9601 cagcgtttac gtttattaaa tagcagcccc ttgtaatgcc tgatgcgacg cttgccgcgt
     9661 cttatcaggc ctacaacgat gcaaaccgta ggtcggataa ggcgtttacg ccgcatccga
     9721 catgttttcc ctcaaatcag aacaacagcg ccagcccgcc gacaatcaca ccagacactg
     9781 ccaccatcgc ccctgtcagc cataaggctt tcgccgggaa cgctttgcgc agcataatca
     9841 gtgacggcaa actcactgcc gggagcgtca tcaacaatgc cagcgccgga gcggttccca
     9901 tacctgccag catcatcgtt tgtacaatcg gaatttctgc tgccgtggga atgacaaaca
     9961 agcatcctgc taccgccatc gccaccaccc acatcaggct gttatcgaca gcaccatcgg
    10021 catgggggaa taaccagacg cgtgcggcac ccaacaccag tactgcaagg atgtaaaccg
    10081 ggatcgtact ccagaaaagc gtccatagcg ccctgcccca gcggctaaaa aaaccgctct
    10141 gtgcttccgg tatgtcaatt tcgaccggtg cctgcgtttg cggtgtttca cgcacccatt
    10201 tttgcaccag cgtcgcaatc agcaacacca tcaccagccc ggccaccaga cgaatcgccg
    10261 caaaacccca gccgaggaca aaccccataa acaccagcgt cgccgggttt aacaccggat
    10321 tgcccatcca gaatgccagc gcaccgccca tcgatacctg ttggcgacgc attcccgccg
    10381 cgaccggagc cgcacagcag gtacacatca tgcccggcag cgaaaacagc gttcccagca
    10441 gcgtgccgcg aaagcgcgat tgcccaagcg tacgcaacaa ccagtcacgc gggatcagca
    10501 cctgaatcaa cgaaccgagg atcaccccca gcaccgccgc tttccatact gcgaggaaat
    10561 agatcatcgc gtaatccaac gccgcttgcc acggattagc atccacctgc gcaaggatag
    10621 atttaccgat actgtgggtt tcggctgcag taaacgcttt gccgtagtaa ggttgccatt
    10681 tcacatacca gagaccggca atgacaacga gaaagaaaag cgcgggtttc caccactgaa
    10741 tgggcgttgc cgcctgagat gaagactgac cagtcatagc attccccagg aataagttat
    10801 gataattgag cgcgtgaata ttacgctcac tatcaattct tgggaataat tattcagctc
    10861 ttttgcgtaa ttctgaagag cttaaaatcg tcacgccttc atgctcaggc cgcatcgcct
    10921 ctgccagcat gacccgcgcc acgtctctcg catcaatgga tttccagtta cctggtaaca
    10981 aacggaacag cggcgcaaaa agcgtttcgt tcatccgctg tttgctacga tcgcccagta
    11041 acatcgacgg gcgagcaatg gtcaatttct gccagttctg ggcaattaat gcttcttcca
    11101 tctccccttt gacgcggtta tagaataacg gcgagtgggc attggccccc atagcactga
    11161 ccaccagcat atgctgcgcg cccagccgcc gcccggttaa tgcggtatcc actaccagcg
    11221 tgtaatcggc atgaataaac gcctctttgc tccccgcttc tcgccgcgtg gtgccgagac
    11281 aacaaaacac aatgtcgatg ggatcggtga cctgcgccag cgcgtcggtc agttgcggat
    11341 catggggatt gaacactcct ggcatatcgc ccaacggacg tcgcgtcggc gcggctatgg
    11401 cgttaacttt cggttcgtta atcaacatcc gcagcaggtg accgcccacc agccccgttg
    11461 agcctgtaat cagtacctga ctcatcttcg ctcctttaca gaattgtctg ccttgcgctc
    11521 cccggttcat gcaccaggct taatagacca tgaggtaatt atccccgatt gtgggaaatt
    11581 cgcctcatcc aatgcaacaa cgcggaggaa gcatgagtaa gaaaattgcc gttttaatca
    11641 ctgatgaatt tgaagattca gaatttactt cacccgcaga cgagttccgt aaagccggac
    11701 acgaagtaat taccattgaa aaacaagcgg gtaaaacggt gaaaggcaaa aaaggagaag
    11761 ccagcgtgac catcgataaa tccatcgatg aagtgacgcc agcggagttt gatgctctgc
    11821 tgctaccggg cggccattca ccggattatc tgcgtggtga caaccgtttt gtcaccttca
    11881 cccgcgattt tgtgaatagc ggcaaacctg ttttcgccat ctgtcacggc ccgcagttgc
    11941 tgatcagtgc cgatgtgatt cgggggcgca aactgactgc agttaaaccg atcattattg
    12001 atgttaaaaa cgcgggcgcg gaattttacg atcaggaagt ggtggtcgat aaagatcagc
    12061 tggttaccag ccggacgccg gacgatctgc cagcgtttaa ccgcgaggcg ttacgcctgc
    12121 tcggtgcctg agtcgcgtag ccaaatcatt tttttaccaa agcccagcgt gttgtcggtg
    12181 aatttgattt catcgagccg gatttcccat accggggccg acagcattct ggcaaccgga
    12241 aagcgacgat tgtacgcctt gcgcgcgagg tcgatttcct caccttccag cctgcggatc
    12301 tcacctttaa actgcacacc gcgaattaac gctaccgttt tcggctgacc gtttaccgtt
    12361 ccggcaactg ccgcctgcgg cccgctcatc tgcgcgtggc gcgttttttc ttccgtcaaa
    12421 atgtagaagg cgactttctg cgcatcaaaa agataaaagg cattagcgca ccagagttcc
    12481 ccttcctgct gcacacacca ggtgacaaca tgttgttttg ccagccaacg gctgatggcg
    12541 gtgagtgttt ccattgctgt tctcttttat actgtgggcc tgaccttaac atgcgatgca
    12601 gacgatgaca ccctggtttc tttacttgat ccgtaccgcc gacaataagc tttataccgg
    12661 gatcaccacg gatgtcgaac gccgctatca gcaacatcaa agcggcaaag gcgcaaaagc
    12721 actacgtggg aaaggtgaac tgacgctggc gttttccgcg ccagtgggcg atcgttcgct
    12781 ggcgttacgg gcagaatatc gcgttaagca actgaccaaa cggcaaaaag agcgtctggt
    12841 agcggaaggc acagggtttg cagaactgtt gagcagtctg caaaccccgg agattaaaag
    12901 cgattgaaat gctcgtgata ctcaaccaga ccagttacgc cattcagcgc gtcatccgct
    12961 aaacgatgta cctggaaggc gctttcagtg cccggccagc ggcaacgcag atcgtgatgc
    13021 gccgccagtt caaagccgaa acgactgtac agcgccggat cgcccagcgt caccactgcg
    13081 gcatagccga actcattaag cgaatcgagt ccttcataga ccagttggcg agccagcccc
    13141 tgtccacggt atttttcatc taccgccagc ggtgccatgc cgacccattg caggtcttcg
    13201 ccctgcacat caaccggact aaatgccaca tagccaatga cctgaccttc gtcatctgtc
    13261 gccaccagcc ccagcgtcag aaagccatct tcacgcagat cgtgaaccag cttcgcttcc
    13321 gcatcacttt cgaatgagcg acgcagcagg gcatcaatac ccggcgcatc aatgggaatt
    13381 tctactcgaa ttagcatggt tcacctaccg atgtctgttt ggtttcaggc gcggttttca
    13441 ttcccgcctc aacaaaatcc gccagttgca gcagcatcat gcgtaaggct ttcggcattt
    13501 gctccagttc aatggcgtcc atcaggtttt tcacatacag ccccagctcc gtatcgcctt
    13561 caatcaccag ccgacgctgg aagaagagcg tatctggatc ttgtttacgc gccgcaatca
    13621 tcagcagatc gctggcgtcg gcactaaaac tcacatcagc ttgcgcgttc tggctgacca
    13681 ccagtttgcc attcaccacc gaggtaaacc attgcaggtc aatatcacgc acatgaatac
    13741 ttaaccagcg gccttcaaga aactccagct cgccatcatc cagcgcctga cggaattgcc
    13801 agcttaagac ctgctcaaga acctggcgtt ttagcgcaaa tggcgtcagt tttaccggta
    13861 cactcaacag agatggcccc aaatgcacaa tacgggaacg cagtttatcc aacacgagtt
    13921 ttactccctg tttcaacaat catcctattt tgccatatca gaaaaataac atagcggtat
    13981 aaatcaacaa ttccatatga aattgctgct accaccaata caactttaac tgccttaaat
    14041 caaaaattgt cgcagcaagg ttaactaaaa tcccagttcg ttaacatttt tgcgttttga
    14101 tagcgcaacc ttcaggaaaa attatggagc tgctctgccc tgccggaaat ctcccggcgc
    14161 ttaaggcggc catcgaaaac ggcgcagatg ctgtttatat cgggctaaaa gatgatacca
    14221 atgcccgtca cttcgccggc cttaacttta ccgagaaaaa attgcaggaa gcggtgagtt
    14281 ttgtccatca acatcgccgc aaacttcaca tcgcgattaa cacttttgcg catccggacg
    14341 gttacgcccg ttggcagcgc gccgtggata tggcggcgca gctgggtgcc gacgcgctga
    14401 tcctcgccga cctcgccatg ctggaatacg ccgccgagcg ttatccgcat attgagcgcc
    14461 acgtatcggt gcaggcttcg gcgaccaatg aagaggcgat taacttttat catcgccatt
    14521 ttgacgttgc ccgcgtggtg ctgccgcgcg tgttgtcgat tcatcaggtg aaacagctgg
    14581 cacgggtcac acctgtacca ctggaagtgt ttgctttcgg cagcctgtgc attatgtcgg
    14641 aaggccgttg ctatctgtcg tcgtatctga cgggtgagtc gcctaacacc gtgggcgcgt
    14701 gttctccggc ccgtttcgtg cgctggcaac aaacgccgca ggggctggaa tcccgcctga
    14761 acgaagtgct gatcgaccgt tatcaggacg gcgaaaacgc aggttatccg acgctgtgta
    14821 aagggcgtta tctggtggac ggcgagcgct atcacgcgct ggaagaacca accagtctca
    14881 ataccctgga actgctgccg gagttaatgg cggcgaatat tgcttcggtg aaaattgaag
    14941 gccgccagcg tagcccggcg tatgtcagcc aggtggcgaa agtctggcgt caggctatcg
    15001 accgttgtaa ggccgatccg caaaacttca taccgcaaag cgcgtggatg gagacgctcg
    15061 ggtcgatgtc cgaaggcacg cagaccaccc ttggcgcgta tcaccgtaaa tggcagtgag
    15121 aagagcaatg aaatattcct tagggccagt gctgtggtac tggccaaaag agacgctgga
    15181 agatttttat cagcaggccg ccaccagcag cgccgacgtg atttatcttg gtgaagcggt
    15241 atgcagcaag cgccgggcaa ccaaagttgg cgactggctg gagatggcaa aatcgctcgc
    15301 cggaagtggc aagcagattg tgctctccac gctggcgctg gtgcaggcat cctctgaact
    15361 gggcgaactg aaacgctatg ttgagaacgg tgagtttctg attgaagcca gcgatctcgg
    15421 cgtggtgaat atgtgcgctg aacgcaaact gccgttcgtc gccgggcacg cgctgaactg
    15481 ctacaacgcg gtgacgctga aaatattgct caaacagggc atgatgcgct ggtgtatgcc
    15541 ggtggagctt tcccgcgact ggctggtgaa tctgcttaat cagtgcgatg agctgggcat
    15601 tcgcaaccag tttgaagtgg aagtcctgag ctacggtcat ctaccgctgg cctactccgc
    15661 ccgctgcttt accgcgcgtt cggaagaccg cccgaaagat gagtgtgaaa cctgctgcat
    15721 taagtatccg aacgggcgca acgtgctgtc gcaggaaaac caacaagtgt ttgtactcaa
    15781 tggcattcag accatgagcg gctacgttta caacctcggt aacgagctgg catccatgca
    15841 ggggctggtt gatgtggtcc gcctgtcacc gcagggtact gacactttcg cgatgctcga
    15901 cgccttccgc gctaatgaaa atggcgctgc gccactaccg ttgacggcaa acagcgactg
    15961 taacggctac tggcggcggc tggccgggct ggagctgcaa gcgtaagtat atagctcact
    16021 ttgttaacaa ctttaactac tctttaatgc agtattaaag actaatcggt aacaaagtga
    16081 gctgttatga ctgataaaac cattgcgttt tcactactcg atctggcccc catccccgaa
    16141 ggttcttcag cgcgggaagc gttctcccac tctctcgacc tcgcccgtct ggctgaaaag
    16201 cgcggctatc atcgctactg gctggcggaa caccacaata tgaccggcat tgccagtgct
    16261 gcaacatcgg tattgattgg ctatctggcg gcgaatacca ccacgctgca tctggggtct
    16321 ggcggcgtga tgttgcctaa ccactcaccg ttggtcattg ccgaacagtt cggcacgctc
    16381 aatacgctct atccggggcg aatcgatttg gggctgggtc gtgctccggg tagcgatcag
    16441 cgaaccatga tggcgctacg tcgtcatatg agcggcgata ttgataattt cccccgcgat
    16501 gtcgcggagc tggtggactg gtttgacgcc cgcgatccca atccgaatgt gcgcccggta
    16561 ccaggctatg gcgaaaaaat ccccgtgtgg ttgttaggct ccagccttta cagcgcgcaa
    16621 ctggcggcgc agcttggtct gccgtttgcg tttgcctcac acttcgcgcc ggatatgttg
    16681 ttccaggcgc tgcatcttta tcgcagcaac ttcaaaccgt cggctcgact ggaaaaacca
    16741 tacgcgatgg tgtgcatcaa tattatcgcc gccgatagca accgcgacgc tgagtttctg
    16801 tttacctcaa tgcagcaagc ctttgtgaag ttgcgccgtg gcgaaaccgg gcaactgccg
    16861 ccaccgattc aaaatatgga tcagttctgg tcgccgtccg aacagtatgg tgtgcagcag
    16921 gcgctgagta tgtcgttggt aggtgataaa gcgaaagtgc gtcatggctt gcagtcgatc
    16981 ctgcgcgaaa ccgacgccga tgagattatg gtcaacgggc agattttcga ccaccaggcg
    17041 cggctgcatt cgtttgagct ggcgatggat gttaaggaag agttgttggg atagtgtgtc
    17101 ttaacgcggg aagccttatc cgagctggca acgctgtcct acgtagacct gataagcgaa
    17161 gcgcatcagg cattgtgtag gcagcagaaa tgtcggataa ggcaccgctg attactgata
    17221 caccggcagt aaattaaagc tcgataaaat atggaccagt gcgttgccaa cgccaaacac
    17281 cagaatcagc gtaatcatcg gcttgccgcc ccagacgcgg aatttcgggc tgccaaagcg
    17341 tttacgcgat gcgcgggcta acagcgccgg aacaattgcc gcccagatgg tagctgctaa
    17401 accagcataa ccaatggcgt acaggaatcc gttcgggaac agtagaccac ccacaacagg
    17461 cggggcaaag gtcagcaatg ccgttttcaa gcgccccagg gctgagtcat cgaaaccaaa
    17521 cagatctgcc agatagtcaa acaaacccag cgttacgcca aggaacgaac tcgctaccgc
    17581 aaagtttgag aacacgacca gcagaaggtc caggctacgg ctgttcagca cgccgcttaa
    17641 tgcctgtacc agcacatcaa tattaccgcc cttctctgca ataccgataa actccggacg
    17701 cgggatgtta cccatcgtcg ccagcaacca gatggtatac agcgccagtg ccatcagcgt
    17761 accgtacacc aggcatttca cgatggtttt cggatctttg ccgtaatact tcatcaggct
    17821 tggcacgtta ccgtgataac caaacgacgc caggcagaac ggcaatgtca tcagcagata
    17881 cggcgcataa gatgcattgc tttctgcaac gttgaacaat gtcgcaggct gcacatgccc
    17941 caacaggctg ccaaaggtga ggaagaaggt aatgactttc gcccccagca caatcgccgt
    18001 catgcggctg acggccttag tactcaacca caccacaaac gctaccagca gtgcaaaacc
    18061 aaaacccgcc gcccgtgccg ggacgtttag cgacatctct gcgaaggtgt gatgcagaat
    18121 tgaaccactg gcagaaatat aggcataggt caggatatag agcacaaagg caatggaaat
    18181 accgttgacc acgttccagc ctttgcccag caaatctttg gtgatggtgt caaaactcga
    18241 accgattcgg taattcaggt tagcttccag aatcatcaag ccggaatgca gcatacagaa
    18301 ccaggtaaag atcagcgccg ccattgacca gaaaaaccac gccccggaca tgaccactgg
    18361 cagagaaaac atccctgcgc caataatggt gccgccgata atcaccacgc cgccaagcag
    18421 cgacggtgac gtttgggtgg tggttagtgt tgccatgagg gcttctctcc agtgaaaaat
    18481 agtgcgactg cgttgttatg cattgcactg taccagtaca cgagtacaaa agacagaaaa
    18541 aaagccccga tggtaaaaat cggggctgta tatattattt tacagattgt gttcgctgtt
    18601 cagcgatgat tacgcatcac caccgaaacg acgacgaccg gtagagtcat cacgacgcgg
    18661 agcgcggcct tcacgacgtt cgccgctaaa acgacgacca tcaccacggc caccttcacg
    18721 gcgttcaccg ctgaagttac gaccgccttc acgacgttcg ccaccgaaac cacgaccacc
    18781 gccacgacgc tcaccgccag tatgcggctg tgcatcgccc agtaactgca tgttcatcgg
    18841 cttgttgaga atgcgagtgc gcgtaaagtg ttgcagcact tcacccggca tacctttcgg
    18901 cagttcgatg gtggagtgag aagcaaacag cttgatgtta ccaatgtaac ggctgctgat
    18961 gtcgccttcg ttagcaatcg caccaacgat atgacgaact tcaacaccat catcgcggcc
    19021 cacttcaatg cggtacagct gcatatcgcc aacatcacga cgttcacgac gcggacgatc
    19081 ttcacggtca ccacgcgggc cacggtcgtt acgatcgcgc ggaccacggt catcacggtc
    19141 acggaattca cgtttcggac gcatcggcgc atctggcggt acgatcagag tacgttcacc
    19201 ctgtgccatt ttcagcagtg ccgcagccag agtttcgaga tccagctctt caccttctgc
    19261 agtcggctga attttgctca gcagtgcgcg gtattgatcc agatcgctgc tttccagctg
    19321 ctgctgtact ttagcggcga atttttccag acggcgtttg cccagcagtt ctgcgttcgg
    19381 cagttctact tccggaatag tcagcttcat agtacgttca atgttgcgca gcagacgacg
    19441 ctcgcggttc tcaacgaaca gcagcgcgcg gccagcacga cccgcacgac cggtacgacc
    19501 gatacggtga acgtaagact cagaatccat cgggatatcg tagttaacta ccaggctgat
    19561 acgctcaacg tccaggccac gggctgcaac gtcggtcgca atcaggatgt ccagacgacc
    19621 atctttcagg cgttccagtg tctgttcacg cagcgcctgg ttcatgtcac cgttcagcgc
    19681 ggcgctgttg tagccgttac gctcaagagc ttcagccact tccagagtcg cgtttttggt
    19741 acgaacgaag ataatcgccg catcaaaatc ttccgcttcc aggaaacgta ccagtgcttc
    19801 gtttttgcgc ataccccaga cagtccagta gctctggctg atgtcaggac gggtagtcac
    19861 gctggactga atgcgcactt cctgcggctc tttcataaag cggcgggtaa tgcgacgaat
    19921 cgcttccggc atggttgcag agaacagagc ggtctgatga ccttccggga tctgcgccat
    19981 aatggtttca acgtcttcga tgaagcccat gcgcagcatt tcgtcagctt catccagaac
    20041 cagaccgctc agtttagaga ggtccagagt gccacgtttc aggtggtcca gcagacggcc
    20101 cggagtaccg acaacgatct gcggcccctg acgcagggcg cgtaattgca cgtcataacg
    20161 ctggccgccg tatagagcaa ccacatttac gccgcgcatg tgtttagaga aatccgtcat
    20221 tgcttcagca acctgtaccg ccagttcgcg ggtcggtgcc agcaccagaa tctgtggtgc
    20281 tttcagctca ggatcaagat tctgcaacag aggtaaagag aatgctgcag tttttccgct
    20341 ccccgtctgg gccataccca gaacgtcgcg gccattcagc agatgtggaa tacactctgc
    20401 ctgaattgga gatggttttt cgtaacccag atcgttaagg gcttcaagga taggagcctt
    20461 caggcccaga tctgcaaaag tggtttcgaa ttcagccatg tagtacgtgt gcctcaaaat
    20521 taatggcggc cagtctacat aactcatcat gaaattgatc agcaattttc attgaaaagt
    20581 gtgaaccggc tcaaagtagg tgtattaacg aacaacaacg ccctcacccg ttaaggtgat
    20641 ggcaatcaaa aaagattacg ggctgatgtg tacgtcagct attgctggtc cgattctgcc
    20701 aggtcatctt ggtcctggcc caggagcgat aattccaaca atgcgtatcg gtgctcaaca
    20761 aagttatgaa cgttgttggc aaccgccagt ttgaacagtg ccgtggcgct gtccaaatcc
    20821 cccagactta ggtagtactt acctaaatag aagttggttt cactgagatg ctcagcgagc
    20881 gaggtgttat ccgttgcgtc cgccttgagc ctttccatta acgtttgttc gctaatgttg
    20941 cccaggtaga actcgacaat gttccatccc cactgttcct tatccgattt ttcgaagtgc
    21001 tgtttcaaca cttctttagc ctgcttctca tcgagcttct gctcggcgag ataaagccac
    21061 agactacgga aaggatcatt gggatcgtct tgataaaacg ccagcagatc atcttgcgct
    21121 aacttgtcac gaccgccgta atataatgcg atcccgcgat tcaagtgcgc gtagttgtaa
    21181 gttggatcaa gctcaagtac agaatcaaac gcttcatagg cagcatcaaa attgcctgcc
    21241 tgcgttaaat atatgcctaa gtaattgaat acttcaggca tatccggtcg gattgccagc
    21301 gcttgcgaaa aatcgttacg cgctaatgcc ctcagaccga gactatcata caacactccg
    21361 cgctcatata aaagctgtgc gcgttcgtca tcggttaaag cccgactggc aaggatttgt
    21421 tccatacgtg ccagaatcac ttcctgctgt aaagtcggtt gcaatggtac cgcgaggact
    21481 tcacttttac gccaggaagt attactgcat cctgcaagcg taagtgctgt cgcaacgaaa
    21541 caccagcgca aaaaaggctt catttcccac tcccgaagac cacggttgaa tgaacgtcct
    21601 gttcccggtt gctaacaagg cgtcctgccc ggttaaaagc cccccgccgc agcggagggc
    21661 aaatggcaac cttactcgcc ctgttcagca gccggagctt ccggtgctgc agcaggttga
    21721 gactgctcag tcgcttcttt aatgctcaga cggatacggc cctggcgatc aacttccaga
    21781 actttcaccg gtacttcctg acccatctgc aggtaatcgg tcactttctc aacgcgtttg
    21841 tcagcgattt gagagatgtg gaccagacct tctttaccgc cgccgatggc aacaaatgcg
    21901 ccaaagtcaa cgatacgggt cactttacca gtgtagacgc ggcccacttc gatttctgca
    21961 gtgatctctt cgatacgacg aatagcatgt ttcgctttct cgccgtcggt cgctgcgatc
    22021 ttcacagtac cgtcatcttc gatttcgatg gtagtgccag tttcttcggt cagggcacgg
    22081 ataacagagc cgcctttacc gataacgtct ttgatcttat ccgggttgat cttgatggta
    22141 tggatacgcg gtgcgaactc agagatatcg ccacgcggcg cgttgatcgc ctgttccatt
    22201 acgcccagga tatgcagacg cgcaccttta gcctggttca gcgcaacctg catgatctct
    22261 ttggtgatac cttcaatttt gatatccatc tgcagtgcag agataccgtc gcgggaacct
    22321 gcaactttga agtccatatc gcccaggtga tcttcgtcgc ccaaaatgtc agacagtaca
    22381 acgtagttgt cgccttcttt caccagaccc attgcgatac ccgcaacggc agctttgatt
    22441 ggcacacctg cgtccatcag cgccagagac gcgccgcaca cggaagccat agaagaagaa
    22501 ccgttggatt cggtgatttc agacactaca cgtacggtgt acgggaattt gtccatatcc
    22561 ggcatgactg ccagcacgcc gcgcttcgcc agacgaccgt gaccaatttc acgacgcttc
    22621 ggagaaccga ccatgccggt ttcgcctacg gagtacggag ggaagttgta gtggaacagg
    22681 aaggtatcgg tacgttcgcc catcagttca tcaagaacct gcgcgtcacg agcggtaccc
    22741 agcgttgcag taaccagtgc ctgcgtttca ccgcgggtga acagcgcaga accgtgagta
    22801 cgcggcagca cgccagtacg cacatccaga ccacggatca tatctttttc acgaccgtcg
    22861 atacgcggtt cgcctgccag tacgcggcta cgaacaacgt ttttctcgat agcgtgcaga
    22921 atttcaccca gttcgttttc gtccagggtt tcgtcttcag caagcagcgt cgcgatggtt
    22981 tcagatttga tgacatcaac ctgcgcataa cgctcttgtt tgtcggtgat gcggtaagca
    23041 tcgctcaggc gagcttcagc cagtgcagca acgcgcgcgt tcagcgcttc gtttaccggc
    23101 tccggctgcc agtcccaacg tggtttaccg gcttctttca ccagttcatt gatgttctga
    23161 ataacaacct gctgttgttc atgaccgaac actactgcgc ccagcatctg gtcttcgctc
    23221 agcagttcag cttcagattc aaccatcaga acagcggctt cagtaccggc aacaaccaga
    23281 tccagtttgc tctctttcag ctcgtcctga gtcgggttca gtacgtactg gtcattgatg
    23341 taacctacgc gagcagcacc aatcggacca ttgaacggaa taccagacag agacagtgct
    23401 gcggaagcac caatcatcgc gacgatatcc gggttaactt gcgggttaac agaaaccacg
    23461 gtggcgataa cctgaacttc gttgacgaag ccttccggga acagcgggcg aatcgggcgg
    23521 tcaatcagac gcgcgatcag ggtttcgcct tcgcttgggc ggccttcacg acggaagaag
    23581 ctacccggga tacgaccagc agcgtaggta cgctcctgat agttaacggt cagtgggaag
    23641 aagtcctgac ctggtttggc ttttttctgg ccaacaacgg taacaaatac cgcggtgtca
    23701 tccatgctaa ccataacagc ggcagtagcc tgacgagcca tcatgccggt ttccagagtc
    23761 acggtgtgtt ggccgtactg gaatttacga acgatcggat taagcaatgt aatatccttt
    23821 ctctttctta gacagtacct tacggcactg gtgttaatac ccgatcttct gcgcatcctc
    23881 gcgactaatg acaaccctaa cccagctcta tgtgggtaaa gcctctcatt agccgcgcga
    23941 acctctgcaa cggaagatca ttcatagcaa caatacatta gtttccagtg aattgctgcc
    24001 gtcagcttga aaaaaggggc cactcaggcc cccttttctg aaactcgcaa gaattagcga
    24061 cgcagaccca ggcgctcgat gagctgggtg taacgtgcta cgtctttacg tttcaggtag
    24121 tcgagcagtt tacgacgctg agaaaccatg cgcagcagac cacgacggct gtggtgatct
    24181 tttttgtgct ctgcaaagtg gccctgcagg tggttgatct gtgcagtcag cagtgctacc
    24241 tgaacttcgg tagaaccggt gtcgtttgcg tcacgaccaa actcagaaac gattttagct
    24301 gttgcttcag tacttagaga cattttaaaa ctccaaagta tatagaatga aaggacgccg
    24361 atctctaatt cagcgatccc agtgtacgtt acgcaaagtg ttaaacaatt tacgcgacgt
    24421 taagcggcag tattctactc gtagcgacct gttatcgcaa gacggttaac attacgccgg
    24481 gtattcaacc actaggcgac gaggcgcaac gcggccttca tcgtcaattt cgcccatacc
    24541 gataaatttg ccgttctcac cttctgtaac gcgaaccagt ccttccagcg gcgcaccgga
    24601 tgtacgaact gggttaccat ttttgaagta aacagaagac gttaacggaa gattcaccac
    24661 cgggtagtcc gaagctggac tgtccattgg catcagtaat ggatcaagta actccgcagc
    24721 cggaatatcc tgctgttcgg cttgttcgac cagttcacgc agatgttcca gggtcaccat
    24781 ccgttcaacc ggatatttac ttaccgccag acgacgcagg taaataacat gcgcgccaca
    24841 accgagtttt tcacccaggt catcaatgat ggtgcggata taagtgcctt ttgagcagtg
    24901 aatttccagc tccagctcat tgccttcgtg gcgaataaac agcaattcat aaacggtaat
    24961 cgggcgcgct tcacgcggta cttcaatgcc ctgacgcgca tattcgtaca gttttttacc
    25021 ctgatatttc agtgctgaat acatcgaagg gatctgttcg atatcgccac ggaaagtatc
    25081 cagcgccgcc gccagttgct ctgcgctaaa ggttaccgga cgttcttcaa cgatttgtcc
    25141 gtcggcatca gaagtatcgg tacgctgtcc aagacgcgca atgacccgat agcgtttgtc
    25201 ggagtccagc agatactggg aaaacttcgt cgcttccccg aggcaaattg gcaacatgcc
    25261 agttgccagc gggtccagcg caccggtatg cccggcacgg ttggcgttat aaatccgttt
    25321 cactttttgc agcgcatcat tgctggacat accctgcggt ttatccagca acaaaacgcc
    25381 gttaatgtcg cgaccgcgac gacgaggacg actcattagt cctccttgct gtcgtccggg
    25441 ttaacacgac gttcttcgtc atgtttgacc acgctggtca ccaggtttga catgcgcatc
    25501 ccttcaacca gagagttgtc gtagaagaag gtcagttccg gcacgatacg caggcgcatc
    25561 gctttcccca gcaggctgcg gatgaaacca gaagcttctt gcaacgcttt gatgcccgct
    25621 ttaaccgcgt cttcatcttt gtcgttgagg aacgtcacat atactttggc atacgccagg
    25681 tcgcgagaca tttcgacacc ggaaacggtg gtcatcatgc ccaggcgagg atctttaatt
    25741 tcacgctgca ggatgagagc gatctctttt tgcatttcct gcgctacgcg ctgcgggcga
    25801 ccaaattctt tcgccataat aaattctcct gacaaaaaag gggctgttag ccccttttta
    25861 aaattaattt caggtggaag ggctgttcac gttggcctga taagacgcgc cagcgtcaca
    25921 tcaggcaacg gctgccggat gcggcgtaaa agccttatcc ggcctacaac ccagacaacc
    25981 ttaagcgatg gtacgttgga tttcgatgat ctcgaatact tcgatcacat cgccagtgcg
    26041 gacatcgttg tagttcttaa cgccgatacc acattccata ccgttacgga cttcgttaac
    26101 gtcatctttg aagcggcgca gggactccag ctcgccttcg tagataacca cgttgtcgcg
    26161 cagaacgcgg atcgggttgt gacgtttaac cacaccttcg gtaaccatac aacctgcaat
    26221 ggcaccaaat ttcggtgatt tgaatacgtc acgaacttcc gccagaccga taatctgttg
    26281 tttcagttcc ggagacagca taccgctcat tgccgctttc acttcgtcga tcaggttata
    26341 gatgacggag tagtaacgca gatccaggct ttccgcttca atcactttac gtgcagaggc
    26401 atcagcacgt acattaaagc caaccaggat ggcgttggac gccgcagcca gggtggcgtc
    26461 ggtttcggtg ataccaccta cgccagaacc gatgatcttc actttaactt cgtcagtaga
    26521 cagtttcagc aaggagtcgg agatcgcttc cacagaaccc tgtacgtctg ccttcaggac
    26581 aatgttcact tcgtgaactt cgccttcggt catgttggcg aacatgttct cgagtttaga
    26641 tttctgctga cgcgccagtt taacttcgcg gaatttaccc tgacgataca gtgcaacttc
    26701 acgcgctttc ttctcgtcac gtacaacggt aacttcatca cccgcagccg gtacgccgga
    26761 caggccgagg atttccaccg gaatggacgg acccgcttcc agcacttcct gacccagttc
    26821 gttacgcatc gcacgaacac gaccgtattc gaagccacac agaacgatat cgcccttgtg
    26881 cagagtacct tcacgtacca gaacggtagc aaccggacca cgacctttat cgaggaagga
    26941 ttcgataacc gcaccgctcg ccataccttt acgtaccgct ttcagctcca gaacttccgc
    27001 ctgcagcagg atagcgtcca gcagttcatc gatgccggta cccgctttcg cagatacgtg
    27061 tacgaactgg ctttcaccgc cccactcttc cggcaggatg ccatactggg agagttcgtt
    27121 cttaacacga tccggatcgg cttctggttt atcaatcttg ttcactgcaa ccaccaccgg
    27181 tacctgcgcc gctttcgcgt gctggattgc ttcgatggtc tgcggcatca cgccgtcgtc
    27241 ggcagcaaca accaggacta cgatgtccgt tgcctgcgca ccacgtgcac gcattgaagt
    27301 aaacgcggcg tgccccgggg tgtccaggaa ggtgatcatg ccgttttcag tttcaacgtg
    27361 gtatgcacca atgtgctggg taatgccgcc cgcttcgcca gaggcaactt tcgttgaacg
    27421 aatgtagtcc agcagagagg ttttaccgtg gtcaacgtga cccatgatgg tcacaaccgg
    27481 cgcgcgcggt tcagccgcag cacccgtgtc acggtcgctc attaccgcct cttccagctc
    27541 gttttcacga cgcaggataa ctttgtggcc catctcttca gcaaccagct gtgcggtttc
    27601 ctgatcgata acctggttga tggttgccat tgcgcccagt ttcatcatcg ctttgatgac
    27661 ctgagagcct ttaaccgcca tcttgttcgc cagttcgcca acggtgatgg tttcgccgat
    27721 cacaacgtca cggttaacgg cctgagctgg cttctggaag ccttgctgca gggaagaacc
    27781 tttacgtttt ccgcctttac cgccacgtac tgctgcgcgt gcttcttcac gatcagcttt
    27841 tgattcagcg tgtttgttgc ctttcttcgg acgcgctgct ttcgcgttac gaccacggcc
    27901 acggccgcct tcgacttcac gatcgctttc gtcttctgcc tggcgagcat gttgagaagt
    27961 agtgacgtga taatcgctgg aatcttcagt cggttccgcg ttatcagtcc atttgttttc
    28021 ttccgccata cgacgtgctt cttcagcaac gcgacgtgct tcttcttcga gtttacgacg
    28081 cgcttcttct tcggctttac gcttgagctc tgcagcttcc tgctcacggc gggctttttc
    28141 agcctgggcg tttttagtca tatcgtcttg ttgattgctc actttgtctt tttccgcagc
    28201 ttcacgtttc gcttgttcag cagcttcact cttagcttgt tctgcggcct cacgttcagc
    28261 tttttgttgc gcctcgcgtt tagccgattc ttctgcctca cgacgggctt gctcttccgc
    28321 ttcacgctgc gcttgctctt ccgctgcaag gcgttcagcc tcttgcggat cgcgtttcac
    28381 aaaggtgcgt ttcttgcgga cttcgatttg taccgatttg ctttttccac cggtaccagg
    28441 aatgttaagg gtgctgcgtg ttttacgttg cagcgtcaat ttgtccgggc ctgaattttt
    28501 ctgattcagg tggtcaatca aagtctgttt ctcttgtgcg gacacagagt cgtcagcaga
    28561 cttccggata cctgcatcag caaattgctg taccaggcgt tccacggagg tctgtcgctc
    28621 tgcggccagc gttttaatcg ttacatctgt catgctgttc cttcctgcta cagtttatta
    28681 cgcttcgtca ccgaaccagc aaatattacg ggcagccata atcagtgctc cggctttttc
    28741 gtcggtcaac ccttcgatat cagccagatc atcaatgccc tgttcggcga gatcttccag
    28801 cgtacaaacg ccacgggcgg ccagtttgaa tgccaaatca cgatctatcc cttcaaggtt
    28861 cagcagatcg tcagccggtt tgttatcacc gaggctttct tcctgggcct gtgcaatggt
    28921 ggccagtgca tttttagcac gctcgcgcag tgcttcaacg gtcggctcat caaggccttc
    28981 gatttccaac agctctttca tcggcacata ggccagttct tccagcgtcg agaagccttc
    29041 ttctaccaga acagtcgcga agtcttcgtc gatgtcgaga tatttggtga aggtgtcgat
    29101 cgctgcgtgc gcttccgcct gatgcttagc ctgcaggtcg tcaacggtca tcacgttgag
    29161 ttcccaaccg ctcagctgcg aagccagacg cacgttctga ccgttacggc caatcgcctg
    29221 cgccaggtta ccggcttcaa cggcgatatc catggtgtgt ttatcttcat ccaccacgat
    29281 agaagcaacg tctgccggtg ccattgcgtt aatcacaaac tgcgccgggt tatcatccca
    29341 caggacgata tcgatacgct cgccgcccag ttcagtagaa accgcctgaa cacgcgcgcc
    29401 acgcatacct acacaggcac ctaccggatc gatacgttta tcgttggttt tcaccgcgat
    29461 tttcgcacga gaacccggat cgcgagccgc tgctttaatt tcaatcactt cttcgccgat
    29521 ttctggcact tcaatacgga acagttcgat cagcatttcc ggcttggaac gagtgacgaa
    29581 cagttgcgcg ccacgcgctt ccgggcgaac ggaatagagc acgccacgaa cgcggtcgcc
    29641 agggcggaag ttttcacgcg gcagcatatc ttcgcgcagg atcacggctt cagcgttgtt
    29701 gcccagatcc agagagatgt tgtcgcggtt tacttttttc accacgccgg tgatgatttc
    29761 accttcgtgt tcacggaact gatcaaccac catcgcacgt tcggcttcac gcactttctg
    29821 cacgataacc tgttttgccg tctgggtagt gatacggtca aaggtaacag actcaatctg
    29881 atcttcaacg taatcgccca ggttcaggct ttcatcttca taacgtgcgg cttcaagggt
    29941 gatttccttg gtcggctggg tgacttcatc aacaactaac cagcgacgga aggtgtcaaa
    30001 atcaccgctt ttgcgatcga tctgtacgcg gacgtcgatc tcttgttcat attttttctt
    30061 tgttgctgtc gccagcgcgc tttccaatgc ttcgaaaatc ttctcgcgag gtagcgcctt
    30121 ttcattggat acggcttcaa ctacagccaa aatttctttg ttcatcgcgg gcttttcacc
    30181 tcatccagac tattaaaagt ggggaaccag gttcgccttc tggatattac tcagcgcgaa
    30241 cacttcatct ttaccttcga cggtaactgt gatcatttca ccgtctaccg ctttgataac
    30301 gccctgccat ttacgacggt tttgtaccgc catacggaga accagagtca cctcttctcc
    30361 gacaaaacgg gcgtagtgtt cagccgtgaa cagtgggcga tcgagacccg gtgaggagac
    30421 ttccaggtta taagcaacgg tgatgggatc ttcaacatcc agcacagcac ttacctggtg
    30481 gctcacatca gcacaatcat caacattgat gccatcttca ctatcaatat agatgcgcag
    30541 tgtggatgtg cgaccgcgaa taaattcgat gccaaccagt tcaaaaccca gggcctcaac
    30601 tggcgcagta atcatctctg ttaatttttg ctctaatgtg gacaagccca cccccaagac
    30661 ataaaaaaag ggcttaaagc ccagttattc tgtagtcaga taacaaaaaa ccccgataaa
    30721 tcggggcttt atataactga accctataac cgcaactgcg gtctggagca ctttccagaa
    30781 ggattttttc aaatcccact acgaaggccg aagtcttcac agtatatttg aaaaaggact
    30841 ctaagggaaa gtggttgcgg gggccggatt tgaaccgacg accttcgggt tatgagcccg
    30901 acgagctacc aggctgctcc accccgcgcc tgaaacgtgg caaattctac tcgttttggg
    30961 taaaagatgc aaatactgct gggatttggt gtaccgagac gggacgtaaa atctgcaggc
    31021 attatagtga tccatgccac attttgtcaa cgtttattgc taatcacgtg aatgaatatc
    31081 cagttcactt tcatttgttg aatacttttg ccttctcctg ctctccctta agcgcattat
    31141 tttacaaaaa acacactaaa ctcttcctgt ctccgataaa agatgattaa atgaaaactc
    31201 atttattttg cataaaaatt cagtgagagc ggaaatccag gctcatcatc agttaattaa
    31261 gcagggtgtt attttatgac gacgattctc aagcatctcc cgctaggtca acgtattggt
    31321 atcgcttttt ctggcggtct ggacaccagt gccgcactgc tgtggatgcg acaaaaggga
    31381 gcggttcctt atgcatatac tgcaaacctg ggccagccag acgaagagga ttatgatgcg
    31441 atccctcgtc gtgccatgga atacggcgcg gagaacgcac gtctgatcga ctgccgcaaa
    31501 caactggtgg ccgaaggtat tgccgctatt cagtgtggcg catttcataa caccaccggc
    31561 ggcctgacct atttcaacac gacgccgctg ggccgcgccg tgactggtac catgctggtt
    31621 gctgcgatga aagaagatgg cgtgaatatc tggggtgacg gtagcaccta taaaggaaac
    31681 gatatcgaac gtttctatcg ttatggtctg ctgaccaatg ctgaactgca gatttacaaa
    31741 ccgtggcttg atactgactt tattgatgaa ctgggcggcc gtcatgagat gtctgaattt
    31801 atgattgcct gcggtttcga ctacaaaatg tctgtcgaaa aagcctactc cacagactcc
    31861 aacatgcttg gtgcaacgca cgaagcgaag gatctggaat acctcaactc cagcgtcaaa
    31921 atcgtcaacc caattatggg cgtgaaattc tgggatgaga gcgtgaagat cccggcagaa
    31981 gaagtcacag tacgctttga gcaaggtcat ccggtagcgc tgaacggtaa aacctttagc
    32041 gacgacgtag aaatgatgct ggaagctaac cgcatcggcg gtcgtcacgg cctgggcatg
    32101 agcgaccaga ttgaaaaccg tatcatcgaa gcgaaaagcc gtggtattta cgaggctccg
    32161 gggatggcac tgctacatat cgcttatgaa cgtctgttga ccggtattca caacgaagac
    32221 accattgagc agtatcatgc acatggtcgt cagttgggcc gtctgctgta ccaggggcgt
    32281 tggtttgatt cccaggcgct gatgctgcgt gactctctgc aacgctgggt tgccagccag
    32341 atcactggtg aagttaccct ggagctgcgc cgtgggaacg attattcaat cctgaatacc
    32401 gtctcagaga acctgaccta caagccagag cgtctgacga tggaaaaagg cgactcggtg
    32461 ttctcgccag atgatcgtat tggtcaattg accatgcgta atctggatat cactgatacc
    32521 cgagagaaac tttttggtta tgccaaaact ggcctgctct cctcctctgc cacttcaggc
    32581 gtgccgcagg tggagaatat ggaaaacaaa gttaagtaat atcatagctt tcgtgctaaa
    32641 ggccgcaaat gcggcctttt tttacggcta agagaatgtg actgcgttta ggggccacca
    32701 tgtccgaatt cggaattaaa gtaaaagtta aatgaaataa agtaaatttc agcgcattct
    32761 atttttactt tatgttttta tgctcagtaa aaggaatgac cgatgaatgg tgaatatttt
    32821 gtacgcctcg cattacatac tctgaaatgc actcaaaaag atttagcaag ccacttaggt
    32881 gtttcctcaa cacaaattag caagtggaaa aaaggggaac acatgtccgc tgatatggaa
    32941 aaaaagttca gaaatattac acaaatagga gattattctc ctcaactagt tgaatggacg
    33001 ggctcagtcg cgaatgctga gaaatgggat cgacttatcc attttcttgc tcgacgagct
    33061 atggacgatg ctgaaacggg ttatataaca caacttctta ctgacgaaga tggttttttg
    33121 gttgaagaaa caattgatgt attaaagcga ataggttttc ctacacctct ttcctttcct
    33181 gaaggactta atattgacga cgataatgct gatgaagaag aggctttttg ggagatatta
    33241 gagagcaatg ctcattgttc tgtaattaat gatatttatc atgctctgaa tgatgtttat
    33301 ggtttctaca tagcatatgt tgatgagttg attcaggacg acgatttaga tgtctattca
    33361 agcgaagcca tcaatattca gagttcattg atatcacttg ctgcatgcaa aattgaaatt
    33421 gatactcctg ttgcgtcaaa ctttaaagaa tttcgttata gagttaaaaa agactacgaa
    33481 aattggttga atcaactcaa gatgatggca ttcagggcgg gaattccgtt aagagccgaa
    33541 cttctggaaa tggtttataa caccgctgac caacttagcg ttgcagcaga agccgaacgt
    33601 ttcgatttca ataaatctcg cattcatcca gatatataca tgaacgagat ccttactggc
    33661 atgagaataa ttcaccaagt gcttccggta ataatgcaaa agttagaaat tacagatttc
    33721 aaactagatg aaacagattt atgcctgggt aaatagttat caaattaaga atacttagcc
    33781 tggcttataa aacgaaaaaa gacgcttttc agcgtctctt ttctggaatt tggtaccgag
    33841 gacgggactt gaacccgtaa gccctattgg gcactaccac ctcaaggtag cgtgtctacc
    33901 aattccacca cctcggcacg gatactactt tttagttcgg gatatcgctg gtcggcttag
    33961 ccggagcagc tggttgagtt tgttcggttt tcaccggtgc actcagattt tcccattcgc
    34021 tacctttatt ggttttgttg ctattgatgt tacccagcac cagactgatg atgaagaata
    34081 acgttgccag cagcgccgtc atgcgggtca tgaagttacc agaaccactt gaaccaaaca
    34141 gcgtagcgga agcgcctgct ccgaaggagg ctcccatatc agcgccttta ccttgctgca
    34201 gcatgatcag accaacaagg ccaattgcca caataaggaa aacaactaaa agagcttcgt
    34261 acataatcaa cctgttcctt gcggagttgc cgcgtaccaa tgcttcaacc aataaagcgg
    34321 gagtttttaa tctttcccac tgaagcgggt gtgaatacta accaaagcga atgaccttcg
    34381 caagggcaat tttatcgcat tgtatcaact gcggaaaaaa acagcaaaac cgatatgttc
    34441 gctgagaaaa aaggcgacaa tcgccgcctt tttagccagt tatctaacgc tttaaacggc
    34501 ttttactgca tcggcgatgc ggtgtgcaaa ttcagtcacc tgcgcttcgt cttcgccttc
    34561 caccatcacg cgaattaacg gttcggtgcc ggatttacgc agcaacacgc gtccacggtt
    34621 gcccagcgca gcttcaacct ctgcggtcac ggctttaact gactcatgct caagtggatc
    34681 gccgctacct gcggtgtaac gtacgttaac cagaatctgc gggaacattt tcatgccgct
    34741 gcaaaggtcg tgcaggctca tatggttacg tgccatcgcc gccagcacct gcaagccagc
    34801 aacgatgccg tcaccggtag tagttttatc cagcaggatc acatgaccgg aattctctgc
    34861 accgatacgc cagcctttct cctgcatttt ttccagtacg tagcggtcac ccactttcgc
    34921 gcgcgcaaat ggaattccta actgtttcag cgccagttca agccccatgt tgctcatcaa
    34981 tgtacccaca gcgccaccac gcagctggcc ctgacgaaga ccttcacgcg cgatgatata
    35041 catgatctga tcgccatcga ctttattgcc ttcatggtca accataatca cgcgatcgcc
    35101 gtcgccgtcg aaggcaatac cgagatccgc tttttcaccc agcacacgag cctggagcgc
    35161 gcgaacgtcg gtagccccca cttcggcatt gatgtttaca ccgtttggct cacaaccgat
    35221 agcgataacg ttcgccccca gttcgcgcag cacgttcggc gcgatgtgat aagtcgcacc
    35281 gtttgcacaa tccaccacaa tcttcagttc actgaggcta agttcgttcg ggaatgtggc
    35341 tttgcaaaac tcgatatagc gacccgcggc atcaacgata cggctggctt tacccagttc
    35401 tgccgaatca acgcagctga tctccttttc catttccgct tcgatggcct cttctaccgc
    35461 atccggcagt ttggtgccgt cgatagagaa gaatttaatc ccattatcgt agaacgggtt
    35521 atgcgatgca gatatcacaa ttccggcctc tgcgcggaag gtacgcgtca gataagccac
    35581 ggccggtgtt ggcatcgggc cagtgaagag tgcggaaagg cccgctgccg ccagacccgc
    35641 ttccagtgct gactccagca tatagccaga aatacgcgtg tctttaccaa taatgatctt
    35701 acgggagccg tggcgcgcca gcactttacc cgcggcccaa cccagcttaa gcacaaaatc
    35761 aggtgtgatc ggcgcatccc ctacacgacc acgaatccca tcggtaccga aatatttacg
    35821 attactcata gcgtttgttt tcctttgcag acagagtggc ttccaccacc cgcatcgctt
    35881 ctacggtttc tttgacgtca tgaacacgaa taatgtgcgc gccttgcatt gcggcaatga
    35941 ccgcacaggc cagactaccg ctcaggcgct cggacggccc cacgttcagc agctgcccaa
    36001 tcatcgattt tcgtgacata cctaccaaca gcggcaggtt gaaatggtga aattcagcca
    36061 ggcgcgccag taatgaatag ttatgggaga gatttttacc gaaaccgaat ccggggtcga
    36121 gcaacaattt ctcttttgcg atacccgcct gctcgcaacg tgctatttgc tcaataaagt
    36181 agcgattcac ttctgcaaag acatcgtcat acttcggagc ttcctgcatg gtttttggat
    36241 ttccctgcat atgcatcaga caaaccggta aaccggtttc tgcagccgcc tccagagcgc
    36301 caggttcgga aagggagcgg atatcgttaa taatgtgagc gccaactttc gctgactcac
    36361 ggatgacttc tggtttggat gtatctaccg agatccagac ttcgaagcgt tgagcaattg
    36421 cctcaaccac aggaataaca cgttgcaact cttcttcaac gctaacttcc gccgcccctg
    36481 ggcgcgtgga ctcgccacca acgtcaatga tcgtcgcgcc agcgttgatc atcagattcg
    36541 catgtttcac cgcatctatc agcgagttat gcgtgccacc atccgaaaag gaatcaggcg
    36601 tgacgttgag gatccccatt acgtgaggat ggctaaggtc cagtgaagtg ccctgggcaa
    36661 agagtttcat ggtgttatcc ctggtatgaa ttgataagaa aaaaccccgg agcacgcccc
    36721 ggggttttcg gtacaaatac agtcatctga tgcgggaact tacttgtcgc ctaactgctc
    36781 tgacatggtg ttacccgggt tcggcgtacg cggttcatca accggacgag gagcctttgg
    36841 actaccattg tcgccagaat tgttagaagc gcctggttct tcccagcccg ctggcggacg
    36901 tacatcgcga cgtgccatca ggtcatcaat ctgcggtgcg tcgatagtct catatttcat
    36961 gagagcatct ttcatcgcat gcagaatatc catattgtcg gtcagaagct gacgcgcacg
    37021 attatagtta cgctcaatca gtgctttcac ttcctggtcg atgatacgtg cagtttcatc
    37081 ggacatatgt ttcgctttcg ctacgctacg gccgaggaac acttcacctt cttcttccgc
    37141 gtacagcagt ggacccaatt tctcagagaa gccccactga gtcaccatgt tacgcgccag
    37201 gttggtcgca actttaatat cgttggacgc accggtagaa acatgttccg gcccgtagat
    37261 gatctcttct gccagacgac caccgtacag cgtagaaatc tggctttcca gtttctgacg
    37321 gctggcgctg attgcgtcgc cctcaggcaa gaagaaagtc acacccagcg cacgaccgcg
    37381 tgggataatc gtcactttgt gcaccggatc gtgttccggc accaggcgac cgataatcgc
    37441 atgacccgct tcgtggtaag ccgtcgattc tttctgcgct tccgtcatca ccatggagcg
    37501 acgttccgca cccatcatga ttttatcttt cgctttctcg aactcaacca tcgacacaac
    37561 gcgtttgttg ccacgagcag cgaacagtgc cgcttcgttt accaggttcg ccaggtcagc
    37621 accggagaaa ccaggagtac cacgggcaat gattgccgcg tcgatatcgg gtgccaatgg
    37681 tacgcgacgc atgtgaactt tcaggatctg ctcacgaccg cgaacatctg gcaagccaac
    37741 cacaacctga cggtcgaaac ggccaggacg cagcagggcc gggtcaagaa cgtccggacg
    37801 gttagtcgcg gcgataacga tgataccttc gttaccttcg aagccatcca tctcaaccag
    37861 catctggttc agagtctgtt cacgttcatc gtgaccaccg cccagaccag cgccacgctg
    37921 gcggcctacg gcgtcgattt catcgataaa gatgatgcac ggtgccgctt tcttcgcctg
    37981 ttcgaacatg tcacgaacac gggatgcacc cacaccgacg aacatttcta cgaagtcaga
    38041 accggagata gtaaagaacg gaactttcgc ttcgcctgca atcgctttcg ccagcagcgt
    38101 tttaccggta cccggaggac cgaccatcag gacgcctttc gggatcttac cgccgagttt
    38161 ctggaagcgg ctcggctcgc gcagatattc aaccagttca gcaacttctt cttttgcttc
    38221 gtcgcagccc gcaacgtcag caaaggtcgt tttgatctga tcttccgtca gcatgcgcgc
    38281 tttgctctta ccaaacgaca tggcaccttt gccaccgccg ccctgcattt gacgcatgaa
    38341 gaagatccac acaccaatca gcaacagcat cgggaaccag gagatgaaga tagaagccag
    38401 caggcttggt tcttcaggcg gttcaccgac aaccttgacg ttcttggtca acaggttatc
    38461 cagtaatttc ggatcctgaa ccggaatgta agtggtataa cggttactat ctttcttggt
    38521 aacgttgatt tcacgtccgt tgatacgcgc ttcacgaacc tggtcgttat tgacctcttg
    38581 taggaaggta gagtaatcca ccttacggcc attagactcg ctgggcccaa agctctggaa
    38641 tactgacatc agcacaacgg caatgaccag ccagagtatt aggtttttcg ccatgtcact
    38701 caagggatta acctcttatt acaactgtgt taaaaacagc gtcaggatac tctatatcca
    38761 gcatctttca aactttcgtc tgaaatctcc cggttagggt ttacgcccgg tcgctacaat
    38821 atacacttcc cgcgaacgtg cacgagaaga gtccggctta cgaactttga ccttcgtaaa
    38881 cagggagcga atttccctta gatactcatc gaaaccttcg ccctggaaca ccttcactac
    38941 aaaactgcca cctggcgcta atacatcacg acacatttct aacgccagtt ccaccagata
    39001 catggcacgg gggatatcca ccgccggtgt tccgctcatg tttggtgcca tatcggacat
    39061 gacaacctgg actttgctgt cgccaacgcg ctccagcagt gctttcatca ccagttcatc
    39121 acgaaaatcg ccctgaagaa agtccacacc aacgatagga tccataggta aaagatcgca
    39181 agcgatgatg cggcctttgc cgccaatttg ggtgaccaca tattgtgacc aaccacccgg
    39241 agcagcacca aggtcgacaa ccgtcattcc cggtttaaag agtttgtcac tttgctgtat
    39301 ttcatcaagt ttaaaccagg cacgggaacg taaccccttt ttctgtgcct gttgaacata
    39361 tttatcgcta aagtgttcct gaagccagcg gctggagctg gcagaacgct tcttacctgt
    39421 catttaactt tcccatgggg ataactcatc gtaaccaatt gcgtaaattt ttacgcgcct
    39481 atttggtgat atatgggaga tggcggtaga atgacccgtt ttcaatccca acgtaagcaa
    39541 aaaatacgat gaatctgagt actaaacaaa aacagcacct gaaaggtctg gcacatccgc
    39601 tcaagccagt tgttctgctt ggcagtaatg gtttgaccga aggggtgctg gccgagattg
    39661 aacaagcgtt agagcaccat gaactcatca aggtgaaaat cgccaccgaa gatcgcgaaa
    39721 ctaaaacctt gatcgtggaa gctatcgtgc gcgaaaccgg cgcctgtaat gtacaggtca
    39781 tcggtaaaac gctggtgctt tatcgcccaa ctaaagaacg taaaatctcg ctgccacgct
    39841 aagattatcc taaagttaca cacatttgct gtgtaaaacg aggggttttc cgcaggcagg
    39901 agagcaaaat gccacgctct gttcgttgat aaaaggccgc atagcggcct ttttcctttc
    39961 tttacaatac ctcaacatct tgagtattgg gtaattctta caggtattcc accttaatta
    40021 cttcaaattc tacttcgccg cccggcgttt tgatgaccac aacatcatct tcttctttgc
    40081 cgatcaggcc acgagcaata ggcgagttta cagaaatcag gttttgttta aagtcagctt
    40141 cgtcatcgcc aacgatgcga taagtctgtt cttcgtcaga atccagattc agcaccgtta
    40201 cggtagcacc aaaaataacg cgcccattgt tgggcatttt ggtgacatca atcacctgcg
    40261 cgttcgacag cttggcttcg atgtctttaa tacggccttc gcagaaaccc tgctgttcac
    40321 gagctgcgtg gtattcggcg ttttctttca ggtcgccatg ctcacgcgct tccgcgatag
    40381 cagcaatgat ttcaggacgg cgcacagatt tcagaaaatc cagctcttcg cgtaattttt
    40441 cagcgccgcg taaggtcatc ggaatagctt gcatttgtta tacctcttga atatttctga
    40501 tggggcaagt cttcacccac cccggctgtt cggcctgccc ggcataactc ctcaccggga
    40561 ccagaagcaa aaaaataccg acccgggtac aagtcccagg tcagctacaa ttcacatttt
    40621 gatagtcatt ttaccctgaa gttcccgaag ggtcatcgtt tactttatag ggcgttgcgc
    40681 cgtagtatga cggctcgatt ccaggttgtt agcgcgagat tatgcgattt tccagattta
    40741 tcatcggatt gaccagctgt atagcgttca gtgttcaggc cgcgaatgtt gatgagtacg
    40801 ttactcaact ccccgctggt gccaaccttg ccctgatggt gcaaaaagtc ggcgcgtcgg
    40861 cccccgctat tgattaccac agtcagcaga tggcgctgcc cgccagtacg cagaaagtga
    40921 ttactgcgct ggcggcgttg attcaactcg gccccgattt tcgtttcacc acgacgcttg
    40981 aaaccaaagg caatgtggaa aacggcgtac ttaagggtga cttagtggcg cgatttggtg
    41041 ccgatccgac gttaaaacgt caggatattc gcaatatggt cgcgactttg aaaaaatctg
    41101 gcgtcaacca aatcgatggc aatgtgttga tagatacctc cattttcgcc agccacgata
    41161 aagcccccgg ctggccatgg aatgacatga cacaatgctt tagcgctccg cctgccgctg
    41221 ccatagttga ccgcaactgt ttctccgtct cgctctacag tgccccaaaa cctggtgata
    41281 tggcttttat acgcgtggca tcttattacc ccgttacgat gttcagccag gtacgcaccc
    41341 tcccccgagg ttctgccgaa gcgcaatact gcgaactgga tgtcgtacca ggcgacctga
    41401 accgctttac gctgacggga tgcctgccac aacgttctga gccgctcccg ttggcttttg
    41461 ccgtgcagga tggagccagc tatgccgggg caattctgaa agatgagtta aaacaggcag
    41521 gtatcacctg gagcggaaca ctgctgcgcc agactcaggt taacgaacct ggaacggtag
    41581 ttgccagtaa acagtcggcc ccgttgcacg atctgcttaa gattatgctg aaaaagtcgg
    41641 acaacatgat cgccgatacg gttttccgca tgataggcca tgcgcgcttc aatgtgcctg
    41701 gaacatggcg ggccgggtcg gacgccgtgc gtcagatcct gcgccagcaa gccggtgtcg
    41761 atattggaaa caccattatt gccgatggtt cagggctttc gcggcataac ctgattgccc
    41821 ccgccaccat gatgcaggtg ttgcaataca ttgcccaaca cgacaatgaa cttaacttta
    41881 tctccatgct gccgctggcg ggctatgacg gctctttgca gtaccgtgca ggtctgcatc
    41941 aggcgggcgt ggatggaaaa gtctcagcga aaaccggttc gttgcagggg gtatataacc
    42001 tggcgggatt cattaccaca gcgagcgggc aacgaatggc gtttgtacaa tatctttctg
    42061 gctatgcagt agaaccggcg gatcagcgta atcgccgtat tccgttagtg cgttttgaaa
    42121 gccgtttgta taaagatatt tatcagaaca attagtcaaa aagaaacccc ggcacatggc
    42181 cggggttttg ctgattaacg cttgtaaatg aactcaacgc cttcttcgtc gtcttcgtcc
    42241 cagtcatcat cccagtcctc atcatcctct tcagcaatct cttcaagctg ctggcgatga
    42301 taatcatccc acatgaattc gactttctct ggctgtttcg cttcttcagc ctgcacgacc
    42361 gggttttcaa tgataaaggt catcacatcc cagcagagat ctttcacacc cagtccactc
    42421 gccgcagaga tcagataata tttatcttcc cagcccagcg cttcagcaat cgctttcgct
    42481 ttctcttcag cttctgcctt atccagcaga tcgatcttat tgaacactaa ccaacgcggt
    42541 ttcgccgcca gatcctggct gtatttttcc agctcgctga taataatacg cgcgttttca
    42601 accggatcgg tgccgtcaat cggatcgata tcgatgaggt gcaacaggac gcggcaacgt
    42661 tccaggtgct tcaggaagcg gatacccaga cctgcgcctt ccgcagcgcc ttcaatcagt
    42721 cctggaatat cggcaacaac gaagctcttt tcgttgtcca ttcgtaccac acccagactt
    42781 ggcaccagag tggtaaacgg ataatccgcc actttcggtt tagccgccga taccgcgcga
    42841 ataaaggtcg atttacccgc gtttggcatc cccaacatac cgacgtcagc cagcagcatc
    42901 agctccagca gcagctcacg cttatcaccc ggcgtaccgt tggttttctg ccgtggtgta
    42961 cggttaacgg acgatttgaa acgggtattg cccagaccgt gccagccgcc cttagcaacc
    43021 agcagacgct gaccgtgttt agtcatatcg cccatggttt caccagtacc ttggtcgatt
    43081 atacgcgtac ctaccggcac tttaatcgtc acgtctttac cgcgcttacc ggtacaatcg
    43141 cggcttgcgc cgttctgacc gcgctctgca cggaaagatt tttcaaaacg ataatcgata
    43201 agcgtgttca ggttttcgtc ggcttccatc catacgtcac caccatcacc accgtcgccg
    43261 ccatccgggc cgcctttcgg aatatacttt tcgcggcgga agctcacgca accattaccg
    43321 ccatcacctg caacgaccag aatcgatgct tcatcaacaa acttcatttt attctccgta
    43381 aatcattcgc ctgcgcgggg ttgcgatacc accgttgtat gcttacgtaa tccgccccaa
    43441 atacgatgac caatggcgga atacatcgcg cccgcaacca cgacaaacgc accgagataa
    43501 cctaaaaggt ttaacatcgg tctggcgaag aaatcgggcc aggccagtga taaaagatct
    43561 gaaaaaaaca gcgtaaacag tggggtgagc gtgatgatcg cgctcacctg cgctgcctgc
    43621 cagcgagcca tcgcttccgc cagggcgcca tatcctacca atgtattcag tccgcaaaaa
    43681 attaaacatg cgagctgcca gtggctaagc tgcgctatca ctccaggctt tgccagaggg
    43741 aagagcgcaa ttgtacataa agcgtacagt aaaaacagga tctgcggtga ggccagccga
    43801 cgcaataaaa ccttttgcgc cacgccataa ctcacccaaa ccgtcgccgc accgacccca
    43861 aagataactc cccaggtgta atcggtgagc tttgtaaata tctcgaccag actggtgtta
    43921 aaaaacatca ccaggccgct caggagcatc aatgccccta caacctgagt gctgcgcatt
    43981 ttctctttca ggataaatac gctggcaacc atcatgcaaa ctggcgagag ttgcccaatc
    44041 acctgcgaag cggtcggact caggtattgc aaggatgagc tgaacaggat gaagttccca
    44101 aacagcccgg cggtcgccac tgccaacaaa atcaaccagc gtggcttacg aaacacgcgt
    44161 aatggcggca acctcttctt caccgcaaga atggcaccaa ggccaatact cgccatcaag
    44221 aaacggtaaa acacgattgt cggaggttcc atcacctcca gcacctgctt cattgcgatt
    44281 ggcaacgccc cccagcaaat tgctgtggtg agcgccaaaa gaatgccaat gcctgcctgc
    44341 tgcttcatac cgtattccct gcaaagcgca tttgcggatt aacgtcgcac catgcgcatt
    44401 tttaccggtt atcgaatgta aaaagccccg caacgtgttg cggggctttc atccgttacc
    44461 gggacgcgaa aaacttattc agcttcgatg ctgataaatt tacggttttt cgggccttta
    44521 acttcgaatt tcactttacc gtctgcttta gcaaacagag tgtggtcacg accgcaacct
    44581 acgttagcgc cagcgtggaa tttggtacca cgttgacgaa cgatgatgct acccgccaga
    44641 acggattcgc caccgaaacg cttaacgccc aggcgtttag cttctgaatc gcgaccgtta
    44701 cgtgtggagc cgccagcctt tttatgtgcc atttgaaatc tctcctcagg tcttaggcgc
    44761 tgatgccagt aattttcaca tcagtgaacc actgacgatg gccctgctgc ttacgatagt
    44821 gtttacgacg acgaaactta acgattttaa ctttctcgcc acgaccgtga gcaacaactt
    44881 cagctttgat tacgccgcca tcaacgaaag gaacgccgat tttgacttct tcaccgtttg
    44941 cgatcatcag cacttcagcg aactcaacag tttcgccagt tgcgatgtcc agcttttcca
    45001 ggcgaacggt ctgaccttcg cttactcggt gttgtttacc accactttgg aaaaccgcgt
    45061 acatataaaa ctccgcttcc gcgcacacct tttcaatgat tcagagtgcg ctataaatat
    45121 tcacaatagg gcgcgaatat tacgcaaaac gcacgccttt gacaagtgct acagtcaata
    45181 cacgaagaaa aaaaacacaa cttgtacggt aacgtttatc tgtgccattt tttcagtaca
    45241 atcaccctat attcctaacc ataaacccta agttgccttt gttcacagta aggtaatcgg
    45301 ggcgaaaagc ccggcttttg cgatgaattt agaaaaaatc aatgagttaa ccgcgcaaga
    45361 tatggcgggt gttaatgcgg caatccttga gcagcttaat tccgacgtcc aactgatcaa
    45421 tcagttaggc tattacatcg tcagcggcgg cggtaaacgt attcgtccga tgattgctgt
    45481 actggctgca cgagctgttg gctatgaggg aaatgcgcat gtcaccattg ctgccctgat
    45541 cgagtttatc cacacggcga ctctgctaca cgacgacgtt gtggatgaat cagatatgcg
    45601 caggggtaaa gctaccgcca acgctgcatt tggcaatgcc gccagcgtgc tggtaggcga
    45661 ttttatttat acccgcgcgt tccagatgat gaccagcctc ggttcgctca aagtgctgga
    45721 agtcatgtca gaagccgtaa acgtcatcgc agaaggtgaa gttctgcaac tgatgaacgt
    45781 taacgatccg gacatcactg aagaaaacta catgcgcgtt atctatagca aaaccgcgcg
    45841 tctgtttgag gctgccgcgc agtgttccgg gattctggct ggctgtacgc cggaggagga
    45901 gaaaggcctg caggattatg ggcgctatct cggcactgct ttccagttga tcgacgattt
    45961 actcgattac aatgccgatg gcgaacagtt aggtaaaaat gtcggcgacg atctgaacga
    46021 aggtaaaccg acgctgccgc tgctgcacgc gatgcatcat ggcacaccag aacaggcaca
    46081 gatgatccgt accgccatcg aacagggtaa cggtcgccat cttctggaac cggttctgga
    46141 agcaatgaac gcttgtggat ctcttgaatg gacgcgtcag cgtgccgagg aagaagcaga
    46201 caaagccatc gcagcgttac aggtgctccc ggacacccct tggcgagaag cactcatcgg
    46261 cctcgcgcac atcgctgttc aacgcgatcg ttaatcccct cccctcatcc cgcgcagcgc
    46321 gcgggatggt tccagtaaat tccataaaaa cacttattca gctctataac acccgaaaaa
    46381 cagggatgtg catctttata tattctgaat attcacactc tttacaggaa ctttttagag
    46441 caataggcca tcaggagtat agtgatgctc gacagaagaa gtgttctgaa tgaaagcgac
    46501 taacttaagg agtgaggaaa gatggaaagt aatttcattg actggcatcc cgctgacatc
    46561 attgcgggtt tgcgcaagaa gggaacgtca atggcggcgg agtctcgcag aaatggtttg
    46621 agttcctcaa cgctggcaaa cgcattatcg cgcccatggc cgaaaggaga gatgattatt
    46681 gcgaaagccc tgggaactga cccctgggtt atctggccat cacgctacca tgatccgcag
    46741 acccatgagt ttatcgacag aacgcagttg atgcgtagct acactaaacc gaaaaaatga
    46801 gtggtctggc ggtagccccg cgaacggggc tgccagctct cagacgatta ctcgcctttc
    46861 acacgctcaa tatttgcacc taaagcgcgc agtttgtctt caatgcgttc gtagccacga
    46921 tcgatgtgat aaatacgatc aaccaccgtc gtcccttccg caatacaacc agccagcacc
    46981 aggcttgctg atgcacgcag atcggttgcc ataacctgtg cgccagaaag tttttcaaca
    47041 ccgtgacaaa taacggtatt gctttcgatt tcggcatgcg cgcccatacg gctaagctcc
    47101 ggcacatgca taaagcggtt ttcaaagacc gtttcggtga tgaatccggt cccttctgcc
    47161 accaggttca acagcgtgaa ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg
    47221 gtacgtacgt taacagcctt cggacgtttg ccatgcatat ccaggctaat ccagtcttcg
    47281 ccgacttcga tgtccgctcc agcgtcacgc agtttcgcca gcacggcgtc gagagtatct
    47341 ggctgcgcgt tacggcagat aattttgccg cgagaaatcg ccgccgccac caggaaagta
    47401 ccggtttcga tacgatccgg tagcacgcga tagacaccgc cgcctaaacg ttccacacct
    47461 tcgatgacga tacgatcggt gccctgaccg ctaattttcg cacccagcgt aatcaggaag
    47521 ttcgcggtat cgacgatttc cggttcacgc gctgcgtttt caataatcgt ggtaccttcc
    47581 gccagggttg cagcacacat gatggtcacc gttgcgccaa cgctgacttt atccatcacg
    47641 atatgtgcgc ctttcagacg accatcgacg gaagctttaa cgtaaccttc ttccagtttg
    47701 atggccgcgc ctaattgttc gaggccagaa atgtgtagat caaccggacg cgcaccgatc
    47761 gtacaaccgc caggcagtga aacttgcccc tgaccaaagc gcgctaccag cggccccagt
    47821 gcccagatag aagcacgcat ggttttaacc agatcgtaag gtgcgcagaa tacattaacg
    47881 tcgcgggcat caatatgcac agaaccatta cgttctactt tcgcacccag ctggcttagc
    47941 agcttcattg atgtatcgac gtctttcagt tttgggacgt tctggatctc taccggttct
    48001 tccgccagta gagcggcaaa aaggataggc agagcagcat ttttagcgcc ggaaattgtg
    48061 acttcgccct ggagcttcgt tggcccctga acacgaaatt tatccattta gtttgttctc
    48121 agttaacaat tcatatccgc taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg
    48181 cgatcgcgcg cccactccgc aggggtatac gctttgatcg acacagcatg aatgcggtta
    48241 tccgcaatat attccatcag cgggccatag accgtctgct gttttttaac ccgactcatg
    48301 ccgtcaaaca actcacccac ggcaataacc tgaaagtggc tgccatcgtc ggaaacgtgg
    48361 acttcctgga gggagagagc gttcatcaac acgctctgaa tttcattatt ttccatggga
    48421 tcttcaatca tcagttaata aaccagcgaa acatcttaga gcaaagttgc gctggcataa
    48481 ataagcaaaa agcctcgctg ataaatcaga caaggctcga cttgcaggca ggtttgccgg
    48541 acaggcggtt aacgccatat ccggcctgaa aaaatttaac gaggcagaac atcagcaggc
    48601 aaattataca atttcgccag ggtatacact ttgtcgttta ccccctgaag cgtcacattg
    48661 ttgccctgct ttttcgccag atcgataaga tggagcagca gtgccagtcc ccccgtatcc
    48721 acgcgggaca cacggctaag atcgatgcag gtaatcccct tcaccgcttc ctcacgcatt
    48781 tcccaaagcg gtagcaaaac gtcctgatcc agctctccgg ataacgccag cgtgtcaccc
    48841 gtctgcatcc agctcagtga ctcgctcatt attttttctc ttccagagtg attttctgtt
    48901 gagaaatcga tttcagttgc gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc
    48961 cccactcgtt ttgtttggtg gtgatcatac tgacgccttc agcaatcatg tcgtaagcct
    49021 gccagttgcc cgtctgggag tttttacgcc actggaagtc cagacgcacc ggcgggcggc
    49081 cattcggatc aataatggta acgcgaatcg gcacaatggt tttatcgccc agcggctgtt
    49141 ctggcgcaat ctgataggtt tgaccgtgat acatcgccag cgcctgaccg taagcctgct
    49201 tcaggtactc acggaaagcg gcaaagtagg cttcacgttg agcaggggtc gcactcttgt
    49261 aatactggcc cagcaccagc gcaccggcgt atttcacctg tacgtatggc agcagttcct
    49321 gatcaacaat ggtacgcaga taatccgggt tggcccgaat ttgcggttgc tcattcttca
    49381 ggcgatcgaa cgttttctgc gccgcctcgt ccatcagctt ataaggattg gtctggtctg
    49441 ccgcggttgc cgcactcagc ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa
    49501 acatgcgtcg gttctcctga aattatttcg ttgtacccac aggttcagtg gtttcattat
    49561 tacctggcgc agcagctggc gcatcgccac tattcttatt gtcatcgcct ttactaccgt
    49621 aaaggaactg accaatgaga tcttccagca ccatcgcaga cttagtgtcc tgaattgtat
    49681 cgccatcctt caggatagca gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca
    49741 gatattgttc ccccagcagg ccggaagtac gaatgctcag cgaactggta tctggaatgt
    49801 ggttataacg ttgttcaatt tccagcgtta cgcgtggcag ataggttttc gggtccagcg
    49861 taatatccgc cacccggccc acaacaacgc caccaatact gaccggagag cgcgctttca
    49921 ggccgccaat gttatcgaac gtcgcataaa gcgtgtaggt cggttcagta cgtatggacg
    49981 tcacgttcgc cgccttcagg caaacaaaca gcgccgccag cagtgctgct aataaaaaga
    50041 tacccaccca aatttcattt tttttcgttt gcatgaactc aattcccaaa catcaatgcg
    50101 gtcagcacaa aatccagccc cagaacagcc agagacgagt ggacaacggt gcgagtggtt
    50161 gcccggctaa tcccggcaga cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac
    50221 gtcaccgtga tggcgaacac cacgctctta atcagacagt tgaccagatc catacgccag
    50281 tcgacggcat tttgcattgc cgaccagaag aacccgctat caatgccttt ccaactgacg
    50341 ccgaccagag atccgcccca gatccccacg gcgacgaaaa taaccgtcaa cagtggtaat
    50401 gaaataaccc cagcccagaa acggggagaa ataacccgac gcagcggatc caccgccatc
    50461 atctccatac tggagagttg ctctgtagcg cgcatcaggc cgatttctgc ggttagcgcc
    50521 gaaccagcac gcccggcaaa caacaacgcg gcaaccaccg gccccagttc acgcagtagc
    50581 gataacgcca ccagcatacc cagactggtt tccgcactat aagtggtcag aaccagataa
    50641 ccttgcagcc ccaacaccat tccgatgaac acgccagaaa ctacaataat cagcattgac
    50701 aggacgccga cattatagag ctggcgcacc agcagcggcg catgtttgcg aaattccggt
    50761 ttgccgacca gcgcattgaa taacattaac ccggcccgcc cgaacgttct cagggtttta
    50821 atccctttat gtccgagcga cgccagcaca tttaacagca tgagtggctt aactccctgg
    50881 taaaagatca gcgtgataat cgccggcagg atagcggaac ggaacaggcc cgtcagctat
    50941 cccgtccaga aactgacgta cgcgcggatc aggattcgcc tgcaacgcct gggcactgcc
    51001 atgagcgaca atttttttgt ccgccaggat ccaggcatga tccgcaatac ttaacacttc
    51061 cggcacatcg tgagaaacca ccacacaagt cacgcccagc gcgctgttca gctcagaaat
    51121 cagcttcacc agtacgccca tggtgatggg atcttgccca acaaaaggtt catcaaacat
    51181 gatgagatcc ggctccagcg caatcgcacg cgccagcgct gcacgccgcg ccatcccacc
    51241 ggaaagttca gaaggcatta gtttagccgc tccacgcagc cccacggcct ccagcttcat
    51301 catcaccgta ctatgcaaca atggcgcggg aagttgggta tgttcgcgca gtggataggc
    51361 gacgttgtca aatacgttca tatcagtgaa caacgccccg gactgaaata acatgctcat
    51421 ccgtttgcgc actgtataca ggcgcgaacg agacatcgcc ggaatattct caccatcgaa
    51481 aaggatctca ccatgatctg gtgcgatttg cccgccaatc agacggagta gcgtcgtttt
    51541 accgatgccc gatggcccca tgatcgccgt gatcttccct cgcggcacgg tcagggaaat
    51601 attatcgaag atgcagcgat tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt
    51661 cgccacagac tgctccataa ttcacccttc gtcttgcgtt gattttctaa gcatggcgct
    51721 caatttaacc ttgaacccaa catatttaca gaatattacc cgccgtggtt agcgaaagct
    51781 ggcatttgtt ttacttttta gccgcataaa gtcaaaatta agaatccgtt acggctttct
    51841 gaaaatcttc agcggaccgg cgagtatacc tgaagaaagg acgttagatg cttttagcta
    51901 cggcactgtt aattgttggt ttacttttgg tcgtttacag tgccgaccgc ctggtttttg
    51961 ccgcgtctat tctttgccga acctttggca tcccgccgct gatcatcggc atgacggtgg
    52021 tcagtattgg tacatcgtta ccagaaatca tcgtctcgct tgccgcgtct ctgcacgaac
    52081 aacgcgattt agccgttggt acagccctcg gctcaaacat tatcaatata ttgctgatcc
    52141 tcggtctggc tgcgctggtt cgtcctttta ccgtccattc tgatgttcta cgccgtgaat
    52201 tacccttaat gttgttggtc agcgtggtgg ccggttccgt actctatgac ggacaactta
    52261 gtcgcagcga tggtatcttt ctcctctttc tggctgtgct atggctgctg ttcattgtta
    52321 aacttgcacg tcaggctgaa cgtcagggga ctgacagcct gaccagagag cagcttgcag
    52381 agctgccgcg tgacggcgga ttgcccgtcg cgtttttatg gctcggcatt gcgcttatca
    52441 tcatgccagt ggccacgcgg atggtggttg ataacgccac ggtgctggcg aattactttg
    52501 ccatcagcga gttgacgatg ggtctgacgg caattgctat cggaaccagc ctgccggaac
    52561 tggcaaccgc aatagcgggg gttcgcaaag gtgaaaacga cattgctgtc ggaaatatca
    52621 ttggcgcaaa catttttaat attgtcatcg tgttgggttt acccgcgctg ataacgccag
    52681 gagagattga tccattggcg tacagtcgtg actacagcgt gatgttgctg gtgagcatta
    52741 tttttgcgtt gctgtgctgg cggcgctccc cgcaaccggg ccgtggtgta ggggtattat
    52801 taactggcgg atttatcgta tggctggcga tgttgtactg gttatcgcca atactcgttg
    52861 aataactgga aacgcattat gtcgcacgta gagttacaac cgggttttga ctttcagcaa
    52921 gcaggtaaag aagtcctggc gattgaacgt gaatgcctgg cggagcttga tcaatacatc
    52981 aatcagaatt tcacgcttgc ctgtgaaaag atgttctggt gtaaagggaa agttgtcgtc
    53041 atggggatgg ggaaatcggg gcacatcggg cgcaaaatgg ccgcaacgtt tgccagcacc
    53101 ggtacacctt catttttcgt ccatcctggt gaagccgcgc atggtgattt aggcatggtc
    53161 accccacagg atgtggtgat tgctatctct aactcaggtg aatccagcga aatcacggcc
    53221 ttaattccag tgcttaagcg tcttcacgta ccgttaatct gcatcaccgg tcgcccggag
    53281 agcagcatgg cgcgcgccgc agatgtgcat ctgtgtgtta aagtagcgaa agaagcctgt
    53341 ccgttagggc tggcaccgac cagcagcacc accgccacgc tggttatggg cgatgccctc
    53401 gctgtcgcgc tgttaaaagc acgcggcttt actgctgaag attttgcgct ctcacaccca
    53461 ggcggcgcac tgggtcgtaa acttctgctg cgcgtaaacg atattatgca tacgggcgat
    53521 gagatcccgc atgttaagaa aacggccagt ctgcgtgacg cgttgctgga agttacccgc
    53581 aaaaatcttg gtatgactgt catttgcgat gacaatatga tgattgaagg catctttacc
    53641 gacggtgatt tacgccgtgt cttcgatatg ggcgtggatg ttcgtcagtt aagtattgcc
    53701 gatgtgatga cgccgggggg aatacgtgtg cgccctggca ttctggccgt tgaggcactg
    53761 aacttaatgc agtcccgcca tatcacctcc gtgatggttg ccgatggcga ccatttactc
    53821 ggtgtgttac atatgcatga tttactgcgt gcaggcgtag tgtaaagatt caaggataaa
    53881 caacaatgag caaagcaggt gcgtcgcttg cgacctgtta cggccctgtc agcgccgacg
    53941 ttatagcaaa agcagagaac attcgtctgc tgatcctcga tgtcgatggc gtactgtcag
    54001 atggcctgat ttatatgggc aataatggcg aagagctgaa agcgttcaat gttcgtgacg
    54061 gttatggcat tcgttgtgcg ctcacctctg atattgaagt cgctatcatt accgggcgaa
    54121 aggctaaact ggtagaagat cgttgtgcca cattggggat cactcacttg tatcaggggc
    54181 agtcaaacaa actgatcgcc tttagcgatc tgctggaaaa attggcgatt gccccggaaa
    54241 atgtggctta tgtcggcgat gatctcatcg actggccggt aatggaaaaa gtgggtttaa
    54301 gcgtcgccgt ggccgatgcg catccactgt tgatcccgcg cgccgattac gtgacgcgca
    54361 ttgctggcgg tcgtggcgca gtgcgcgaag tttgcgactt attactcctg gcgcagggca
    54421 aactggatga agccaaaggg caatcgatat gagtaaagcc agacgttggg ttatcattgt
    54481 gctatcactg gcggttctgg tgatgatcgg cattaatatg gccgaaaaag acgataccgc
    54541 ccaggtggtc gtcaacaaca atgatcccac ctataaaagc gagcatacgg acacgctcgt
    54601 ctataaccca gaaggggcac taagctatcg attgattgct caacacgttg aatattattc
    54661 cgatcaggcc gtttcgtggt ttacgcagcc ggtacttacc acgtttgata aggataaaat
    54721 cccgacatgg tccgtaaaag cagataaagc caagctgacc aatgaccgga tgctctattt
    54781 atatggacac gttgaagtca acgcacttgt gccagactct caacttcgca gaatcacgac
    54841 ggataacgcg cagatcaatc tggtgacgca ggatgttacc tctgaagacc tcgtcacgtt
    54901 atacggaaca acatttaact ccagcggtct gaaaatgcgc ggcaacttac gcagcaagaa
    54961 cgccgagctg attgaaaagg ttagaacatc ctatgaaatt caaaacaaac aaactcagcc
    55021 ttaatcttgt gcttgccagc tcacttctgg ccgccagcat tccggcattt gccgtaaccg
    55081 gagacactga tcagccgatc cacattgaat cggaccagca atctcttgat atgcaaggca
    55141 acgtggttac ctttaccggt aatgtcatcg tcacccaggg caccatcaaa attaatgccg
    55201 acaaagtggt cgttacccgt ccgggcggcg aacaaggtaa agaagtgatt gacggctacg
    55261 gtaaaccggc aacgttctac cagatgcagg acaacggtaa acccgttgaa ggtcacgctt
    55321 cccagatgca ctacgaactg gcaaaagatt ttgtcgttct gacgggtaat gcttatctgc
    55381 agcaggtcga tagcaacatt aagggcgata agatcactta cctggtaaaa gagcagaaaa
    55441 tgcaggcttt cagcgacaaa ggcaagcgcg taaccaccgt tctggtgccg tcgcagctgc
    55501 aggacaaaaa caacaaaggc cagaccccgg cacagaagaa gggtaattaa ttcgttatgg
    55561 caacattaac tgcaaagaac cttgcaaaag cctataaagg ccgtcgcgtg gtagaagacg
    55621 tcagcctgac cgtcaactcc ggggaaattg tcggtctgct ggggccaaac ggtgccggta
    55681 agaccaccac tttctacatg gttgtaggca ttgtgccgcg cgatgcgggc aacatcatta
    55741 ttgatgatga cgatatcagt ctgctgcctc tgcatgcacg cgcgcgccgc ggtatcggct
    55801 atctgccaca ggaagcctcc attttccgtc gcctcagcgt ttacgataac ctgatggcgg
    55861 tactgcaaat tcgtgacgac ttgtctgctg aacaacgtga agaccgcgcg aacgagctga
    55921 tggaagagtt tcacattgag cacctgcgtg acagcatggg gcagtcactc tccgggggtg
    55981 aacgtcgccg tgtagaaatt gcccgcgcac tggctgcgaa tccgaaattt attctgctcg
    56041 acgaaccgtt tgccggggtt gacccgatct cggttatcga cattaaacgc atcattgagc
    56101 acctgcgcga cagcggcctg ggcgtgctga tcactgacca caacgtgcgt gaaacactgg
    56161 cggtttgtga acgcgcttat atcgtcagtc aggggcattt gatcgcccac ggcacgccta
    56221 cagaaatctt acaagacgaa cacgttaagc gtgtatacct tggggaagac ttcagactct
    56281 gatagggtag aagtttgcga cgttttagca ggagagtacg attctgaaca tgaagcaagg
    56341 tttgcaactc aggcttagcc aacaactggc gatgacgcca cagctccaac aggcaattcg
    56401 tctgttgcag ttgtcgacgc tggaacttca gcaggagcta cagcaggcgc tggagagtaa
    56461 tccgctgctt gagcaaatcg acactcatga agaaatcgac acccgcgaaa cgcaagacag
    56521 tgaaacgctg gacaccgccg acgcgctcga acaaaaagag atgccggaag agctgccgct
    56581 cgatgccagt tgggacacca tttacaccgc tggtacacca tccggcacca gcggtgacta
    56641 cattgacgat gagctgccgg tctatcaggg cgaaacgacg cagaccttgc aggattacct
    56701 gatgtggcag gtcgagctga caccgttttc cgacactgac cgcgctattg ctacctctat
    56761 cgtcgatgcc gttgatgaca ccggttatct gactgtcccg ctggaagata ttctcgaaag
    56821 tatgggcgat gaagagatcg acatcgacga ggttgaagcc gtccttaagc ggatccaacg
    56881 gtttgatccg gtcggtgtag cggcaaaaga tctgcgtgac tgcctgctaa tccaactctc
    56941 ccaattcgat aaaaccacgc cgtggctgga agaggccaga ctgatcatta gcgatcatct
    57001 cgatctgtta gccaatcacg acttccgcac tttaatgcgc gtcacgcgtc tgaaagaaga
    57061 tgtgctgaaa gaagccgtca atctgatcca gtcgctcgat ccgcgccccg ggcagtcgat
    57121 ccagactggc gaacctgagt atgtcattcc agatgtgctg gtgcgtaagc ataacggtca
    57181 ctggacggta gaactcaaca gtgacagcat tccgcgtctg caaatcaacc agcactacgc
    57241 ctcgatgtgc aataacgcgc gcaacgatgg tgacagccag tttatccgca gcaatctgca
    57301 ggatgccaaa tggttgatca agagtctgga aagccgtaac gatacgctac tgcgcgtgag
    57361 tcgctgtatc gttgaacagc agcaagcctt ctttgagcaa ggtgaagaat atatgaaacc
    57421 gatggtactg gccgatatcg cccaggctgt cgaaatgcat gaatcgacga tatctcgcgt
    57481 gaccacgcaa aaatacctgc atagtccacg aggcattttt gaactgaagt atttcttttc
    57541 cagtcacgtc aataccgagg gcggcggcga agcttcctcc acggcgattc gtgcgctggt
    57601 gaagaaatta atcgcggcgg aaaacccagc gaaaccgttg agcgacagca agttaacctc
    57661 tttgctgtcg gaacaaggta tcatggtggc acgccgcact gttgcgaagt accgagagtc
    57721 tttatccatt ccgccgtcaa accagcgtaa acagctcgtt tgacccaacc gataaggaag
    57781 acactatgca gctcaacatt accggaaata acgtcgagat caccgaggca ctgcgcgaat
    57841 ttgttacagc caaatttgcc aaacttgagc aatattttga ccgaatcaac caggtctatg
    57901 ttgttctgaa agtggagaaa gtcacccaca cctcagatgc aacactgcat gtaaacggtg
    57961 gtgaaattca tgccagcgcg gaaggtcagg acatgtacgc tgccattgat ggcttaattg
    58021 ataagctggc acgccagttg accaaacata aagataaact gaaacaacac taattgtccg
    58081 ggcaattagc atgtgcatgg cggcctgttg tgcggcacaa cgggccattt gtacggttaa
    58141 tgctccgagc ctgttccact gtttgagtgg gcaggttctt aggtgaaatt atgacaaata
    58201 atgatacaac tctacagctt agcagtgttc ttaacaggga atgtacgcga agccgcgtcc
    58261 actgtcagag caaaaaacgc gccctggaaa tcatcagcga gctggcggcg aaacaactta
    58321 gtctgccgcc tcaggtggtt tttgaagcta tcctgacgcg ggaaaaaatg ggcagtaccg
    58381 gtatcggcaa tggtattgcc attccgcatg gcaaactgga agaagatact ctgcgcgccg
    58441 ttggcgtttt cgttcagctc gaaacgccta tagctttcga tgccatcgac aaccaaccgg
    58501 tggatttact ttttgccctg ctggtgccgg cagaccaaac taaaacgcac ctgcatactc
    58561 tgtcactggt ggcgaaacgt ctggcggaca aaaccatctg ccgccgttta cgcgcagccc
    58621 agagcgatga agagctgtat caaatcatta cggataccga aggtactccg gatgaagcgt
    58681 agttattcgg taatgtctct tttagacgtt gtgaggagaa acagtacatg gtactgatga
    58741 tcgtcagcgg acgttcaggt tcaggtaaat ctgtcgccct gcgtgcgctg gaagatatgg
    58801 gtttttactg cgtggataac cttcccgtag tgttgttacc cgatctggct cgaactctgg
    58861 ccgatcgcga gatttctgcc gccgtcagca ttgatgttcg taatatgccg gagtcaccag
    58921 aaatattcga acaggcgatg agtaacctgc ctgacgcttt ctcaccgcaa ctcctgttcc
    58981 tggatgccga ccgtaatacc ttaattcgtc gttacagtga cacgcgccga ctgcatccgc
    59041 tttccagcaa aaacctgtcg ctggaaagtg ctatcgacaa agaaagcgat ttgctggaac
    59101 ctctgcgttc gcgagcggat ctgattgtcg atacctcaga aatgtccgtt cacgagctgg
    59161 ctgaaatgct gcgtacccgt ctgctgggta aacgtgaacg cgaactgaca atggtctttg
    59221 agtctttcgg cttcaaacac ggtatcccta tcgatgcaga ttacgtcttt gacgtgcgct
    59281 tcttgccgaa cccgcactgg gatccgaaac tgcgtccaat gacaggtctt gataagcccg
    59341 tcgccgcgtt cctcgaccgc cacacagaag tacacaattt tatctaccag acgcgaagct
    59401 atcttgagct atggttacct atgctggaaa ccaacaaccg tagctacctg acggtcgcca
    59461 ttggttgtac cggcgggaag caccgttcgg tgtatattgc agagcaactg gcagactact
    59521 tccgctcgcg cggtaaaaac gtccagtcac gccatcgtac gctggaaaaa cgtaaaccat
    59581 gaccgtcaag caaactgttg aaatcacaaa caagctgggc atgcatgccc ggcctgcaat
    59641 gaagctgttt gaattaatgc agggttttga cgctgaagtg ctgttacgta atgacgaagg
    59701 caccgaggct gaagccaata gcgttattgc actgctgatg ctggattctg ccaaaggacg
    59761 gcagattgaa gttgaagcca ccggtccaca ggaagaggaa gcactggccg ccgttatcgc
    59821 cctctttaat tctggttttg atgaagatta atcttcatca ctttgacatt caaatccctt
    59881 caagctcccc cgacaataag aaaagcgcgt actgaaatcg ttctcaatca acgtcatttg
    59941 tacattttgt gcgcttttca ctttcagaag aaccttaaga aaaccttaag aggcattgtt
    60001 taggttttgt ttaagttaat cgaccatact ggagatcgtc agaaaatatt tccaggagat
    60061 ggcatgattc gcttatctga acaaagtccc ctgggcaccg gaagacatcg gaaatgttat
    60121 gcgcatccgg aagatgccca acgctgtatt aagattgtct accatcgtgg cgatggtggc
    60181 gataaagaga tccgccgtga gttaaagtat tacgcgcatc ttggtcgccg gttaaaagac
    60241 tggagtggaa taccgcgcta tcacggtacc gttgagacgg attgcggaac cggttatgtc
    60301 tacgatgtga ttgccgattt cgacggcaat ccctccatta cgctgaccga atttgctgaa
    60361 caatgccgtt acgaagaaga cgttgcacaa ctgcgccagt tactgaaaca gctgaagcgt
    60421 tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc cgcagaatat cctctgtcat
    60481 cgcatcagtg agtcagaggt tatccccgtc gtctgcgaca acattggtga aagcacattg
    60541 attccactgg caacctggtc gaagtggtgt tgtctgcgta agcaagagcg gttatggaaa
    60601 cgatttatcg cccaaccggc gctggctatt gccctgcaga aagatctgca acctcgtgag
    60661 agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca gctggtgctg ttgcataaac
    60721 gactcaccgc ctaactgata catctgccgt aaaatccacg cctgacggct acgcacgtag
    60781 cccgatggcg cggagacttt gaaacgaagt ggattaggta atacagctgc cagtaatgca
    60841 gcttccgatc gggtaagttt gctcgcgggt ttgtggaaat aacgttgtgc cgcagcttcg
    60901 acgccaaaca caccgtcgcc aaattcggcg atattcaggt aaaccgtcag gatacgcttt
    60961 ttgctccaga ctgtttctat ccccagcgtt aatccagctt ccagcccttt tcgaacccag
    61021 ctgcgcccat cccataaaaa gagatttttg gctgtctgct gagaaatcgt tgaagcaccg
    61081 cgaatacggt tttcattgcg ctcgttgtgc gccagggctt tctcaatgga agcaacatca
    61141 aagccccagt gctcaggaaa tttctgatct tctgcggcaa tcaccgccag tcccatccat
    61201 ggcgagattt gatccatact gacccagtca gaatgtgcca cgtaacgaaa attgccatgc
    61261 agccaggcgc tgacctgtcg ctcgaccatt accgctgaga agggaacagg cgcaacacta
    61321 aacaacgcga tgcccccgcc ccagaaaacg gcgaggacaa ccattaaccg caaaagaaaa
    61381 cggcgaacga aactaaacac cgttaagcgg cttttactca ttcagccaga accagcacgc
    61441 gggaaaccag cttatcaatg ccgctcgccg cttctgcaat gttctgcgcc agcatatatg
    61501 ctggggtggt gacaatctta ttgtcttcat caaccacgat atcatcgaca ggacagggca
    61561 catgctccgc gcccatctct tccagcactt ctgcggtatc gatatcagta ccgatggtca
    61621 aacgcagcgg gaaatcgaaa attttcggca gcatcgccgg ggcaatacac ataaaaccaa
    61681 gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt taattcacgg tcaacggtgc
    61741 attcgctacc aagagtggca aaattgctta aattcttcgc cgcgccaaac ccccccggca
    61801 caatcaacgc atccagttca gcggcatcgg cctgggccag aggacggatt tcaccgcgcg
    61861 ttattcgtgc cgcttcaatc agcacgttgc gcgtttccgt catcgcttcg ccagttaaat
    61921 ggttgataac atcaacctgc tgcttatccg gtgcaaagca gaccgcctgc gcaccgctgc
    61981 gtgaaatagc taacaacgtc aacaccgctt catgaatttc agaaccgtca tagacgccgc
    62041 atccgctcag aattacgcca attttcttca ttgtgatcat cctttcgcaa ttgactgaaa
    62101 cacattaatt tttttaataa aaatggtacg catcacacat ttaactgatt catgtaacaa
    62161 atcatttaag ttttgctatc ttaactgcgt gcggcctgaa aaacagtgct gtgcccttgt
    62221 aactcatcat aataatttac ggcgcagcca agatttccct ggtgttggcg cagtattcgc
    62281 gcaccccggt ctagccgggg tcatttttta gtggcttttg ccacccacgc tttcagcact
    62341 tctacgtcgt gacgccactc ttctttcatc tcttcaatcc attcaccgac gttatcttcc
    62401 caggcaggaa ggtcaggaga ctgaatttgc tgacccagct gttgcagatg gcgtaacccc
    62461 actgaccccg ccgcaccttt aattttatgt ccttcctcaa caatgccttt tttatcctgc
    62521 gccgtcagat tcgactccag cacgctgaca tagcccggca tcatcttctc aaacaccgct
    62581 aacccgtcgg tgatcagctt cggtcctaca agttcgagat actgttccag catgggaata
    62641 tcgagcaatg cttctgattt actgttctct tctgtcgtca ccgtactctc ctcatcatcc
    62701 tgggtatccc agaatttctt gatcatcgcg gttagcgccg gaaccgaaag cggcttactc
    62761 agcacatcat ccattccagc attgaggtac tcttgtttgt ctttcagcac gttagcggtt
    62821 aaggccacca gcggcggtaa atcctcgcgc ggataacgct tcgtcagttc acgagagata
    62881 tccagcccgg tcatatctgg caactgaata tccagcaaca ccaggtcgta ttcgcccggt
    62941 ttaaacatct ccagcgccgc cttgccggtc atggcgacat caacgctgtt acctaatttt
    63001 tccagcacag aacgcgcaac aatcacgttc agttcaatgt cttccaccag cagcacattc
    63061 agcgccggta aaggcatatc gtcttcatca aacgcatcat cgacctcttc tgctaccgac
    63121 ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct gttcgctggt aaccgtaata
    63181 tcgccgccca tatttttcgc cagacgacga gaaacggcca gaccaatacc ggtgccggtg
    63241 gcaggtttac cgccatgact gtctttcacc tggtaataca tggcgaaaat tttatccagc
    63301 tcatcctgcg gaatgccgat accagagtct tccacttcaa aatgcagcat atcgccttca
    63361 tcgtagcgca cacgcacggt aacctggcct tgctgggtga atttgacggc gttactgatg
    63421 aggttccaca ggatctgccg taaacgcgtc ccgtcggtaa tgacctgatg cggtaatggc
    63481 agcgtcggct ccaggttaaa gcgcaatcct ttttgttgcg cctgcaatgc ggagagattt
    63541 tccagatcgg caaggaagct ggtgaaatca accggttgat tatcaagctg gaccttgcgc
    63601 cgttccatct tatccatgtc gataatatcg ttaaagatat tccccagcgt gacggccgaa
    63661 acatggatgg tcttgagata tttttcctgc tcggcggtga gttcggtatc cagcagaatg
    63721 cggctcagac cgacgatacc gttcagcggt gtacgcaatt cgtgactgat ggtggagata
    63781 aacgtcgttt tgtcgcggct ggcccgttca agcgcatcct gataccgctt acgctcggta
    63841 atgtcgcgac caaagcccat caaaccgtga cgtttaccca cgcggtcgta gtacggcact
    63901 ttacggattt caaagcaggc tttgcgcccg tccgggtaat ccagccactg ttcataggtc
    63961 agtgacacat tatgacggaa cactttttca tcggtttcaa tgacttttgc ggcggcttcc
    64021 ggtgagtaaa catcagcagg tttcaggtga accagttgtt tttcgctttt tccggtcagc
    64081 agctccatcg cgcggttaca gccggaaaac tctttatctt cgttacgata aaaaaccagg
    64141 tcgggtgaag catcaaggaa ggaacgtaag aatgaggatt gctgctcgag ctgaatttgt
    64201 gtctcttcgc gctctttgat ttcaattttc agttggccga aggtttcctg cagttctgct
    64261 tccgcttttt cacgaacggc aatttcctga tttagctggg caatattatc ttttaactga
    64321 acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt tttgcaccag ccgtgacaga
    64381 cgttgtcgtg actcctccag ttgctcgacg accaccgata gaaagtagac cgcccacggc
    64441 gtaatcagca aaccaaagaa gatagaacga ataacatcaa tgctttcgac ctgaccatgc
    64501 agcaccatgg ttaccgccat ttgtaccaca atggcaagaa cgacgagggc cagcgccagc
    64561 aacattgaga agcgcaccag acctaacttc atcatcaggt caacataata ctgcgccagc
    64621 agacgaattt gcttcattag ggaattcctt cacgacaacc tgccccaata atacccaatt
    64681 ctgacagtta cgttataaat tgtgtgagaa atgcggaatc cccctcaacg acctactccg
    64741 ttggaggtag ccacggacgt cccagcgccg atccctgtac gccatgttca ttcagatagc
    64801 gatccagctc gaccattccc gtccatcgat tttcgcacca cagaggagca agcagcgtcg
    64861 ggcgacgggc gctggcggaa atgcggtggt agatcacttc cggtggcgta tggcgaatca
    64921 tttctccggc agtgagcgtg taatcctcca gttcaatacc gttcaaacgc cccgcttccc
    64981 aggctttcgc cataatgctg cctttcacaa tatgcagcgg atgcagcttt atgccgtcca
    65041 cgccggtttc aaccactcgc tccagcgttt gcaaacattc agcctgccct tcgccaggca
    65101 ggccgacaat taagtgggaa cacaccttca gcccgcgctg acgtgccagc tgggttgtac
    65161 gctgataaca ggcaaaatca tggccgcggt tgatgcgatg cagtgttttg tcgtgggcgg
    65221 tttgtagccc cagctccagc cacacttcgt aaccctggtc cttatattcg caaagcagat
    65281 ccagcaccgc atccggcacg cagtccgggc gggtaccaac acacaaaccg acaatattgg
    65341 cctggctcac cgcctgctga tacatagaac gcagcacctg aacttccgca aaggtgctgg
    65401 tatacgcctg aaagtaggcc agatagcgtt tagcgcggtt aactaaattc gcctggtgcg
    65461 ccagttgctc ggcaatggaa cgatgctgct gcgcttcatc ggcaaacgag gcaacattac
    65521 agaatgtgca gccaccacgc ccgatggtac cgtcacggtt agggcagcta aaaccgccgt
    65581 gtagcgtcag cttatgcacc ttttgcccat aacgacgggt gagatcacca ccaaacatat
    65641 tgactaattt ctgtaactgc ataatctgat agaccgcgcc tcgaaaagag gccaaagcct
    65701 gccattttta gctcaattcg gcgatgacct ggatcaatcg tcccggcctg cttttatcaa
    65761 ctgcataatc aatcaaaatt accgaaattt catgcataat cacataaatc acttttgctt
    65821 atcttgtgtc agattttttt atctccttat ggattttagg caaaaacagt agcatgaaac
    65881 gtcattacca attaaggcag tataaaatgc tggttttctc gtcagttcaa ggcaggataa
    65941 gggttaacac gcctttatga cagtcaggaa ttgactgttt ctctaacgac ttccctttta
    66001 gccttaaaga taaaatccat tttaatttca gtaatttaat aaagaatttt gcgctaaagc
    66061 acatttctgt accaataagc ttgccatttg acctgtatca gctttcccga taagttggaa
    66121 atccgctgga agctttctgg atgagcagcc tgctcatcat atttatgcag taattgagat
    66181 cccctcttca ccgtattaac cgatgcgaaa aggacaacaa gggggcgaat gcgaggcgcg
    66241 cgtatgacac gcaaaccccg tcgccacgct ctttctgtgc ccgtgcgcag cggttcggaa
    66301 gtggggttcc cgcagagcct gggggaggtt cacgatatgt tgtacgataa atcccttgag
    66361 agggataact gtggtttcgg cctgatcgcc cacatagaag gcgaacctag ccacaaggta
    66421 gtgcgtactg caatacacgc actggcccgc atgcagcacc gtggcgcgat tctcgccgat
    66481 ggtaaaaccg gcgacggttg cggcttgctg ttacaaaaac cggatcgctt ttttcgcatc
    66541 gttgcgcagg agcgcggctg gcgtttagca aaaaactacg ctgtcgggat gctcttcctg
    66601 aataaagatc ctgaactcgc cgctgccgca cgccgcatcg ttgaagaaga gctgcaacgc
    66661 gaaaccttgt cgattgtggg ctggcgtgat gtccccacta acgaaggcgt gctgggtgaa
    66721 atcgccctct cctctctgcc ccgcattgag caaattttcg ttaacgctcc ggcaggctgg
    66781 cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc gccgcattga aaagcgtctc
    66841 gaagccgaca aagacttcta cgtctgtagc ctgtcgaatc tggtgaacat ctataaaggt
    66901 ctgtgtatgc cggcggatct gccgcgcttt tatctggatc ttgcggacct gcgtctggaa
    66961 tcggccatct gcctgttcca ccagcgcttc tccactaaca ccgtaccgcg ctggccgctg
    67021 gcgcagccgt tccgctacct ggcgcataac ggtgaaatca acaccatcac cggtaaccgc
    67081 caatgggcgc gtgcgcgtac ctataaattc cagacgccgc tgatccctga tctgcacgac
    67141 gccgcaccgt tcgtcaacga aaccggttct gactccagtt cgatggataa catgctggaa
    67201 ctgctgctgg caggcgggat ggatatcatc cgcgccatgc gtctattagt accacccgcc
    67261 tggcagaaca acccggatat ggacccggaa ctgcgtgcct tctttgactt taactccatg
    67321 catatggagc cgtgggatgg cccggcgggc atcgtgatgt ccgatggtcg cttcgctgcc
    67381 tgtaacctcg accgtaacgg tctgcgtccg gcacgctacg tcattaccaa agacaagctc
    67441 atcacctgcg cctctgaagt cggtatctgg gattatcagc ctgacgaagt ggtcgaaaaa
    67501 ggccgcgtcg ggccaggcga actgatggtg atcgacaccc gcagtgggcg tattctgcac
    67561 tcggcagaaa ccgatgacga tctgaaaagc cgccatccat ataaagagtg gatggagaaa
    67621 aacgtccgcc gactggtacc gtttgaagat ctgcccgatg aagaagtggg tagccgcgaa
    67681 ctggacgacg acacgcttgc cagctaccag aaacagttta actacagcgc ggaagagctg
    67741 gactccgtaa ttcgcgtact gggcgaaaac ggtcaggaag cggtcggttc gatgggcgat
    67801 gataccccat tcgccgtgct ctccagtcag ccgcgcatta tttacgacta cttccgccag
    67861 cagtttgccc aggtgactaa cccgccaatc gacccgctgc gtgaagcgca tgttatgtcg
    67921 ctcgccacca gtatcggtcg tgaaatgaac gtcttttgcg aagcagaggg ccaggcgcac
    67981 cgtttaagct ttaaatcgcc gattctgctc tactccgatt tcaaacagct cacgacgatg
    68041 aaagaggagc actaccgcgc agatacgctg gatatcacct ttgacgtcac taaaaccacg
    68101 ctcgaagcga caatcaaaga gctgtgcgac aaagccgaaa aaatggtacg tagcggcacc
    68161 gtgctgctgg tgctctccga ccggaatatc gctaaagatc gcctgccggt tccagccccg
    68221 atggcggttg gcgcgatcca gacccgtctg gtcgatcaaa gcctgcgttg cgatgccaac
    68281 atcatcgtcg aaaccgccag cgcccgcgat ccgcaccact tcgccgtgtt gctgggcttc
    68341 ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc tgggccgcct ggtagacacc
    68401 catgcgattg ccaaagatta tcgtaccgtg atgctcaact accgtaacgg catcaacaaa
    68461 ggcttgtaca aaatcatgtc caaaatgggc atctccacca tcgcctctta ccgctgctcg
    68521 aaactgtttg aagcggtcgg tctacacgat gatgtagtgg gcctgtgctt ccagggggcg
    68581 gtcagccgca ttgggggggc gagctttgaa gacttccagc aggatctgct gaatctgtcg
    68641 aaacgtgcct ggctggcgcg caagcccatc agccagggcg gcctgctgaa atacgtccac
    68701 ggcggcgaat accacgccta caacccggac gtggtgcgca cgctgcaaca ggcggtacag
    68761 agcggcgagt atagcgacta tcaggaatac gcgaagctgg ttaatgagcg tccggcaacc
    68821 acgctgcgcg atctgctggc aattacgccg ggtgaaaacg cggtcaacat tgctgatgtt
    68881 gaaccggcaa gcgaactgtt taaacgcttc gataccgctg cgatgtctat cggcgcgtta
    68941 agcccagagg cccacgaggc gctggcggaa gcgatgaaca gcatcggcgg taattcgaac
    69001 tccggtgaag gcggcgaaga cccggcgcgc tacggcacca acaaagtgtc gcgcatcaag
    69061 caggtggctt ccggtcgctt tggggttact ccggcgtatc tggtcaatgc cgacgtcatt
    69121 cagattaaag tcgcccaggg cgcgaagcca ggcgaaggcg gtcagttgcc gggtgataaa
    69181 gtcactcctt acatcgccaa actgcgctat tcggtgcccg gagtgacgct gatctccccg
    69241 ccgccgcacc acgatatcta ctctatcgag gacttagcgc agctcatttt cgacctcaag
    69301 caggttaacc cgaaagcgat gatctccgtg aagctggttt ccgaaccggg agtaggcacc
    69361 atcgcgactg gcgtggcaaa agcttatgcg gacttgatca ccatcgcagg ctatgacggc
    69421 ggcaccggcg caagtccgct ttcatcggtg aaatacgcag gctgtccgtg ggagctgggg
    69481 cttgttgaaa cccagcaggc gctggttgct aacggcttgc gtcacaagat ccgtttgcag
    69541 gtcgatggcg gcctgaaaac gggtgtcgat atcatcaagg cggcgattct cggcgcagaa
    69601 agcttcggct tcggcactgg cccgatggtg gcgctcggct gtaaatatct acgtatttgc
    69661 catctgaaca actgcgcaac gggtgtagca actcaggatg acaaactgcg taagaaccac
    69721 tatcacggcc tgccgttcaa ggttaccaat tactttgaat ttattgcccg tgaaacccgc
    69781 gagctgatgg cacagctggg cgtaacgcgt ctggtggatc tgattggtcg caccgacctg
    69841 ctgaaagaac tggacggttt caccgccaaa cagcaaaaac tggcgctgtc gaagctgctg
    69901 gagaccgccg aaccgcatcc aggtaaggca ctctactgca ccgaaaacaa cccgccgttt
    69961 gataacggcc tgctgaacgc gcagttgctg caacaggcga aaccgtttgt cgatgagcgc
    70021 cagagcaaaa ccttctggtt cgatattcgc aacaccgacc gttctgtcgg cgcgtcgctt
    70081 tcaggctata tcgcccagac gcacggcgat caggggctgg cagccgatcc tatcaaagcg
    70141 tacttcaacg gcaccgcagg ccagagcttc ggcgtgtgga acgcgggcgg cgtggaactg
    70201 tacctgaccg gtgatgccaa cgactatgtc ggtaaaggca tggcgggcgg cttaatcgcc
    70261 attcgtcctc cggttggttc cgccttccgc agccatgaag caagcattat cggcaacacc
    70321 tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag gccgcgcggg tgaacgtttc
    70381 ggcgtgcgga actccggtgc tatcaccgtg gtagaaggca ttggcgacaa cggctgtgaa
    70441 tatatgacga gcggtatagt ctgtattctg ggtaaaaccg gcgtgaactt cggcgcgggc
    70501 atgactggcg gcttcgctta cgttctcgat gaaagcggcg atttccgcaa acgcgttaac
    70561 ccggaactgg tcgaggtctt aagcgttgac gctctggcga tccatgaaga gcatctgcgt
    70621 ggtcttatca ccgagcatgt gcagcatacc ggctctcagc gcggtgaaga gattctggcg
    70681 aactggtcaa ccttcgccac taaatttgcg ctggttaaac cgaagtccag tgatgtaaaa
    70741 gcactgctgg gtcaccgtag tcgtagcgca gctgagttgc gcgtgcaggc gcagtaaggg
    70801 gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc tgcagcgcgt tgatccgcca
    70861 aagaaaccgc tgaagatccg caaaattgag tttgttgaaa tttacgagcc gttttccgaa
    70921 ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg gcaacccata ctgcgagtgg
    70981 aaatgcccgg tacacaacta catcccgaac tggctgaagc tcgccaacga ggggcgtatt
    71041 attgaagcgg cggaactgtc gcaccagacc aacaccctgc cggaagtttg cggacgagtc
    71101 tgcccgcaag accgtctgtg cgaaggttcc tgcactctga acgatgagtt tggcgcggtg
    71161 accatcggca acattgagcg ttatatcaat gataaagcgt tcgaaatggg ctggcgtccg
    71221 gatatgtccg gcgtgaagca aaccggtaaa aaagtggcga ttatcggcgc aggcccggca
    71281 ggtctggcgt gtgcggatgt cctgacgcgt aacggcgtaa aagccgttgt cttcgaccgt
    71341 catccagaaa ttggcgggct gctgaccttc ggtattccgg ccttcaagct ggaaaaagag
    71401 gtaatgacgc gccgccgtga aatcttcacc ggcatgggta ttgaattcaa actcaatacc
    71461 gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg attacgatgc cgtgttcctt
    71521 ggcgttggga cttatcagtc aatgcgcggc gggctggaaa acgaagacgc cgatggcgtg
    71581 tacgcagcgc tgccgttcct tatcgccaac accaaacagt taatgggctt tggcgaaact
    71641 cgtgaagaac cgttcgtcag catggaaggc aaacgcgtgg tagtacttgg cggtggcgat
    71701 accgcgatgg actgcgtgcg cacgtctgta cgccaggggg caaagcacgt tacctgtgcc
    71761 tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg aagtgaaaaa cgcgcgggaa
    71821 gaaggtgttg agttcaaatt caacgtccaa ccgctgggga ttgaagtgaa cggtaacggc
    71881 aaagtcagcg gcgtaaaaat ggtgcgtacc gaaatgggcg aaccagactc taaaggccgt
    71941 cgccgcgcgg agatcgtggc agggtcagaa catattgttc cagcagatgc agtgatcatg
    72001 gcgtttggtt ttcgcccaca cagcatggaa tggctggcaa aacacagcgt agagctggat
    72061 tcgcaaggcc gcattatcgc tccggaaggc agcgacaatg ctttccagac cagcaacccg
    72121 aaaatctttg ccggcggcga tatcgtccgc ggttccgatc tggtggtgac cgctattgcc
    72181 gaaggccgta aggcagcaga cggtattatg aactggctgg aagtttaagc tctgtcccag
    72241 cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct ttctttctgg aaagcgcctc
    72301 gcataatctg aagaaaacca atgtaatacg ctcatctgga ttgttaccgg tagatgcccg
    72361 gcgtatagct gattccatta ttcaacgcag cgccatataa agggacagat aatggaatcg
    72421 ctctcagaga gaacctcaac aggctaccag caaatccacg acggtattat tcatctggtc
    72481 gatagcgccc ggacggaaac ggtacgtagc gttaacgcgc tgatgaccgc gacatactgg
    72541 gaaattggcc gacgaattgt cgaatttgaa caaggtggcg aggccagggc tgcgtatggt
    72601 gcgcagctaa tcaagcgact atcaaaggat ttaagtctaa ggtataagcg tgggttctct
    72661 gcaaaaaact tacgccaaat gaggcttttt tacctctttt ttcaacatgt tgaaattcgc
    72721 cagacagtgt ctggcgaatt aacaccattg ggaattccgc agacaccatc tgcagaattc
    72781 ccatcggcaa agatttggca gacgctgtct gccaaatctt tccccctgcc ctggtccacc
    72841 tacgtccgtt tactttccgt taaaaacgct gacgcccgca gcttttatga aaaagagacg
    72901 ctccgctgtg gctggtctgt tcgccagcta gaacggcaaa ttgcgaccca attttatgag
    72961 cgaacactac tgtcacatga caaatcagcc atgctgcaac aacacgctcc taccgagacg
    73021 catattcttc cgcagcaggc gatactcgat ccctttgtgc tcgaatttct ggaattgaaa
    73081 gatgaatact cagaatccga ttttgaggag gcgctgatca accacctgat ggatttcatg
    73141 ctggaacttg gggatgattt tgcctttgtt ggtcgacagc gaaggttacg cattgatgac
    73201 aactggtttc gtgtcgatct gctgtttttc caccgccgtt tacgctgcct actaatcgtc
    73261 gatctaaaag tgggcaaatt cagctatagc gatgccggac agatgaatat gtatctcaac
    73321 tacgccaaag agcactggac gctaccggat gaaaatccgc ccatcggtct ggttctctgt
    73381 gcagagaaag gagccggaga agcacattat gctctggcag gtttgcctaa caccgttctg
    73441 gcaagcgaat ataagatgca actacctgat gagaaacgac tcgcggatga actcgttcga
    73501 acacaggcgg tgttagagga aggctataga cgccgttaat ttcagcggtt tcgaattctg
    73561 cagacggtgt ctgcagaatc tttaaaacga actaagtttt aagccattaa atcagcctta
    73621 accttcacca ccaccttttt aatctcacca ggttcgccaa caacgcatcc tggtttatgc
    73681 ggttcacctg gcataaacac ggcgaacatt cccggtttta agatgatggc ttgctcgtta
    73741 tcaatggtgc tgcaaagctg gtaatcatct tcatggtgga actcttcaca ctgacgcgca
    73801 gtgcctgcca tgccaaacag aatccgttcc tcaccgttta ataacagctg gatatcaatg
    73861 tattgctcgt gcaattccgc ttttttctcg acgggcgatt gagtgttaaa cgtcatgaca
    73921 ttcataaaga tattgtcgcc ctgtaattcg taacgacccg gcgctttttc ttgcggtctg
    73981 gcagctaatg ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc agacggtaat
    74041 gactgtactt cacccatcat cataattttt ctccctgggc caacagcgca gccccaagta
    74101 aacctgcatc atggcggtaa tgcgccgcca gtaaatcaac atgaaatgcc gctggctcct
    74161 gcgccagata cgtttccacc aacgccagat acccttctgc cagaccaacg ctgccaccga
    74221 ccaccacaca ctggcaatca gttgtggctt taatatcagc gatcagcctt gccagcacat
    74281 gtgcggagcg gtgaatcagt tgctgcgcct gctcgtcgcc ctgcccggcg cgtgtgaaaa
    74341 tagttctcgc atccgcgccc atgagttcac cttgcgctgc cgccgcaatg ccgcgaccag
    74401 aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactggacca tgcggatcgg
    74461 caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtaagcagt ttaccgccgc
    74521 tcactacacc gccgccgacg ccagtggaaa cggtgataaa caccatctcg gttacatcgc
    74581 cttccagcgc ctgatactct gcccatgctg cggcctgcgc gtcgttaatg gcaatggtcg
    74641 gcaaattggt aagttgttcc agcgttttga ctaacggaaa gtgtagcaat ccaccgagat
    74701 tatgcggatt cagcgccagc aaactgccgt cgcgaataat tccggttgaa gcgatggcaa
    74761 cccgctgcgc atgagcttgc aacggagaga ctaatgcgga taaggcatca cgcaaggctt
    74821 ctggtgtctg gctggctggc gtaggaagtt cacgacgatc gcggatctgc ccgtcagcgc
    74881 caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg gtcatagcac
    74941 cgcctttttc atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg taatggcaga
    75001 accgaccgtc accgcccacg cgccgtggcg catcgcatct gccgcctgag caggcgtgtt
    75061 gtaacgccct tcggcaatca cccgacatcc ggcgtcgctc aacgttttca ccagcgccag
    75121 atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc caataatttc
    75181 ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg tcatcgccag
    75241 taaaccgtga tggtgaatac gtgccagcag cgtttcaaca ggcaccggac gcgggcggtc
    75301 ggtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga catcttcaat
    75361 ataggccgtg atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc caataatcgg
    75421 cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcaatac gaatggcgac
    75481 cgcgcccgcc tgctctgccg ctaatgccat ggctgcgacg atttcgggtt tatcgagcgg
    75541 gctgtcagga accgactggc aggagacaat caggccaccg ttagcagcga ttttttgatc
    75601 cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac agagaccggg
    75661 caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa cggcacggca
    75721 ccgctgaatg gtttaccgtc gatagcgtca tgagtacgca acgcttccgg gcgcaaccaa
    75781 cgctgaacgc gagaaggcat atccagccca atcagcagga tcaccacgaa cgtcagactg
    75841 aacgagagcg atgccagcgc agtacccaga tccagacgtt gagcgatcaa cgcgccgatg
    75901 attggggcca gtgcaccgcc caatgcgcca acgttgtagg taaagcccag gcccgctgca
    75961 cgctggtcgg tatcgaaata accgccaatc agttttggta agatcccggc gatcccttgt
    76021 ccaagcattt gctggaagaa cagtaacaga ccgagcaccc agacgtttgc gccgccaatc
    76081 gcaaataccg gaataatcag cagctgcgag gccagcaggc tacaaacgta cgctttgcgg
    76141 gttcccagcc agtcaccgag gaagccacct acgcagcatc ccaccgccgc gccaaagcca
    76201 ctaaagaaca gcacattggc tacagtatgc gggttataag ccagatcggt tttcagatac
    76261 gttggcagca gcgcctgaat cggccatgag tagaggaaag caaacaacac gaccaccatc
    76321 agcattacgc ccgttggcca gcgtttgcct gtactctgca ccataaagct gataaagatt
    76381 gcggcgcata acagcccaag aacagcgacg atcgcggcat tttgcaggtt accggcgaag
    76441 cagaaccaca gcgcagtagc cgccgccagt gtcattacga tattggcaat gcgatgttca
    76501 ccacggtaga gaatatccac cattgtgcgt actggtgctt tacctgcgtg tttctctttc
    76561 cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat tggcaaaatg
    76621 ccgataaaga acagcgcacg ccagccccag accggaacca ccaggctata gacctgagcg
    76681 gcaacgacgg cccccacaga gaagcctgaa atcagaaaac cactggcttt gttacgcaga
    76741 tgttttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc cgccatcccc
    76801 atgccgatga ccagacgcgc gataaacatg gtgatgtagc ctggcgcaaa gccgcaggcc
    76861 agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg cccgtagcgg
    76921 tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc tgcggagatc
    76981 agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt gagtaccagg
    77041 gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc ggaaaatgcg
    77101 cgccattgtg cacggttgag atggcgatac cacgggatat tctgggttgt agtactcatt
    77161 gtgagtctcc cgcggtgggc gatgcccaca cgctttggta taaaaattgt agggtacaga
    77221 tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaacaactca cccgcgctct
    77281 tgcatcaact gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca
    77341 aacggtttgc ggcacagcgg cacagaaacg acatccatat aatggaggac agttttcagg
    77401 ccgcggaata cgcccgtttt gatcagtaaa tcaatgactt tattgcattc agtttgcagt
    77461 ttctgcgcgg tctggatatc gccttctttc agcgccttaa cgatcccctg atagcgccag
    77521 cccatgatgt tgtaggtact accgatacca ccatcagcgc ccgccagcag accagaggcg
    77581 aagatttcgt cgtaaccgtt atagagcaca agatcaggat gttcacgacg gatctgctcc
    77641 atctgataga gatcgccaga ggtctgtttc agcgcaccta cgccaggcaa tgtaacaagt
    77701 gtgttgatct gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc
    77761 atcggcaaac catccgccga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag
    77821 ctgaaaggat agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc
    77881 gatgccgcaa gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta
    77941 atcttacctt tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt
    78001 tgtacaaagg cctcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata
    78061 ttgaactgaa ccaggcgacg cagactcgct ttatccagtg cttgttgttg gtcaaaagga
    78121 gtcaggagtg cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg
    78181 cttgtctgat aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc
    78241 atacagaaag cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt
    78301 ggtctgaccg aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg
    78361 atcggcttcg tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata
    78421 actaacgttg ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag
    78481 ccagtcgagc agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag
    78541 cacgcggtgg aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact
    78601 gttgatttcc agtgcttttg ccagcaaatc gatttgctca tcggtggcat gttcagccgc
    78661 atagcgcacc agactggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc
    78721 gggatgagaa aggaaatctt tcgccatgcc ggaaagctca ccgatgatag tgtccgcaga
    78781 aggacgcgag acgcgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa
    78841 cgctgccagc gcttcacgca ccgaaggacg cccgacgtta aagaacgcca tcagttcgcg
    78901 ttcagacggt aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc
    78961 ttcttccacc atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc
    79021 aattgcaggt gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta
    79081 aggtcagtgt gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa
    79141 tggcagggtt ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg
    79201 cgaaaaatta atgtaacgca gataaaaaca tcccgattga attatttata
//
LOCUS       DAZKIV010000004        73768 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZKIV010000004 DAZKIV010000000
VERSION     DAZKIV010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.004"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..237
                     /locus_tag="ACHOT6_000272"
     CDS             <1..237
                     /locus_tag="ACHOT6_000272"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945562.1"
                     /translation="SCNILNLQVFVGRIRRSRRIRHDKAHIDICLSPALRGFFRQKQG
                     DSDEKSVIYLAKLCFIVYPCQTARHKAAECISPF"
     gene            260..1120
                     /gene="mscS"
                     /locus_tag="ACHOT6_000273"
     CDS             260..1120
                     /gene="mscS"
                     /locus_tag="ACHOT6_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFP9945563.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            1259..1894
                     /gene="argO"
                     /locus_tag="ACHOT6_000274"
     CDS             1259..1894
                     /gene="argO"
                     /locus_tag="ACHOT6_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFP9945564.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            1987..2727
                     /gene="yggE"
                     /locus_tag="ACHOT6_000275"
     CDS             1987..2727
                     /gene="yggE"
                     /locus_tag="ACHOT6_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFP9945565.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            2894..3790
                     /gene="ygfI"
                     /locus_tag="ACHOT6_000276"
     CDS             2894..3790
                     /gene="ygfI"
                     /locus_tag="ACHOT6_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9945566.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(3787..5265)
                     /gene="scpC"
                     /locus_tag="ACHOT6_000277"
     CDS             complement(3787..5265)
                     /gene="scpC"
                     /locus_tag="ACHOT6_000277"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFP9945567.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(5289..6074)
                     /gene="scpB"
                     /locus_tag="ACHOT6_000278"
     CDS             complement(5289..6074)
                     /gene="scpB"
                     /locus_tag="ACHOT6_000278"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFP9945568.1"
                     /translation="MSYQYVNVVTINKVAVVEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(6085..7080)
                     /gene="meaB"
                     /locus_tag="ACHOT6_000279"
     CDS             complement(6085..7080)
                     /gene="meaB"
                     /locus_tag="ACHOT6_000279"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFP9945569.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(7073..9217)
                     /gene="scpA"
                     /locus_tag="ACHOT6_000280"
     CDS             complement(7073..9217)
                     /gene="scpA"
                     /locus_tag="ACHOT6_000280"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFP9945570.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDNAAVTAALNVLTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEAAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(9420..10313)
                     /gene="argP"
                     /locus_tag="ACHOT6_000281"
     CDS             complement(9420..10313)
                     /gene="argP"
                     /locus_tag="ACHOT6_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFP9945571.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10696..11355
                     /gene="rpiA"
                     /locus_tag="ACHOT6_000282"
     CDS             10696..11355
                     /gene="rpiA"
                     /locus_tag="ACHOT6_000282"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFP9945572.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKNIV
                     K"
     gene            11611..12843
                     /gene="serA"
                     /locus_tag="ACHOT6_000283"
     CDS             11611..12843
                     /gene="serA"
                     /locus_tag="ACHOT6_000283"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFP9945573.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            13072..13131
                     /gene="ibsC"
                     /locus_tag="ACHOT6_000284"
     CDS             13072..13131
                     /gene="ibsC"
                     /locus_tag="ACHOT6_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFP9945574.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            13464..13523
                     /locus_tag="ACHOT6_000285"
     CDS             13464..13523
                     /locus_tag="ACHOT6_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9945575.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(13624..14226)
                     /gene="fau"
                     /locus_tag="ACHOT6_000286"
     CDS             complement(13624..14226)
                     /gene="fau"
                     /locus_tag="ACHOT6_000286"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFP9945576.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(14247..14430)
                     /gene="ssrS"
                     /locus_tag="ACHOT6_000287"
     ncRNA           complement(14247..14430)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHOT6_000287"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(14472..14801)
                     /gene="zapA"
                     /locus_tag="ACHOT6_000288"
     CDS             complement(14472..14801)
                     /gene="zapA"
                     /locus_tag="ACHOT6_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9945577.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            14969..15547
                     /gene="ygfB"
                     /locus_tag="ACHOT6_000289"
     CDS             14969..15547
                     /gene="ygfB"
                     /locus_tag="ACHOT6_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFP9945578.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            15573..16898
                     /gene="pepP"
                     /locus_tag="ACHOT6_000290"
     CDS             15573..16898
                     /gene="pepP"
                     /locus_tag="ACHOT6_000290"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFP9945579.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            16895..18073
                     /gene="ubiH"
                     /locus_tag="ACHOT6_000291"
                     /gene_synonym="visB"
     CDS             16895..18073
                     /gene="ubiH"
                     /locus_tag="ACHOT6_000291"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFP9945580.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            18096..19298
                     /gene="ubiI"
                     /locus_tag="ACHOT6_000292"
                     /gene_synonym="visC"
     CDS             18096..19298
                     /gene="ubiI"
                     /locus_tag="ACHOT6_000292"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFP9945581.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(19372..19455)
                     /locus_tag="ACHOT6_000293"
     CDS             complement(19372..19455)
                     /locus_tag="ACHOT6_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945582.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            19745..20839
                     /gene="gcvT"
                     /locus_tag="ACHOT6_000294"
     CDS             19745..20839
                     /gene="gcvT"
                     /locus_tag="ACHOT6_000294"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFP9945583.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            20863..21252
                     /gene="gcvH"
                     /locus_tag="ACHOT6_000295"
     CDS             20863..21252
                     /gene="gcvH"
                     /locus_tag="ACHOT6_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFP9945584.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            21371..24244
                     /gene="gcvP"
                     /locus_tag="ACHOT6_000296"
     CDS             21371..24244
                     /gene="gcvP"
                     /locus_tag="ACHOT6_000296"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFP9945585.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            24505..25248
                     /gene="ygfF"
                     /locus_tag="ACHOT6_000297"
     CDS             24505..25248
                     /gene="ygfF"
                     /locus_tag="ACHOT6_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9945586.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(25305..26738)
                     /gene="bglA"
                     /locus_tag="ACHOT6_000298"
     CDS             complement(25305..26738)
                     /gene="bglA"
                     /locus_tag="ACHOT6_000298"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFP9945587.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            26783..27094
                     /gene="yqfB"
                     /locus_tag="ACHOT6_000299"
     CDS             26783..27094
                     /gene="yqfB"
                     /locus_tag="ACHOT6_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFP9945588.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            27258..27917
                     /gene="yqfA"
                     /locus_tag="ACHOT6_000300"
     CDS             27258..27917
                     /gene="yqfA"
                     /locus_tag="ACHOT6_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFP9945589.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHVFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(28113..29093)
                     /gene="ygfZ"
                     /locus_tag="ACHOT6_000301"
     CDS             complement(28113..29093)
                     /gene="ygfZ"
                     /locus_tag="ACHOT6_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFP9945590.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            29336..29602
                     /gene="sdhE"
                     /locus_tag="ACHOT6_000302"
     CDS             29336..29602
                     /gene="sdhE"
                     /locus_tag="ACHOT6_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFP9945591.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            29583..29990
                     /gene="ygfX"
                     /locus_tag="ACHOT6_000303"
     CDS             29583..29990
                     /gene="ygfX"
                     /locus_tag="ACHOT6_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFP9945592.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(30030..30551)
                     /gene="fldB"
                     /locus_tag="ACHOT6_000304"
     CDS             complement(30030..30551)
                     /gene="fldB"
                     /locus_tag="ACHOT6_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFP9945593.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            30663..31559
                     /gene="xerD"
                     /locus_tag="ACHOT6_000305"
     CDS             30663..31559
                     /gene="xerD"
                     /locus_tag="ACHOT6_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFP9945594.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            31584..32294
                     /gene="dsbC"
                     /locus_tag="ACHOT6_000306"
     CDS             31584..32294
                     /gene="dsbC"
                     /locus_tag="ACHOT6_000306"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFP9945595.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLDVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            32300..34033
                     /gene="recJ"
                     /locus_tag="ACHOT6_000307"
     CDS             32300..34033
                     /gene="recJ"
                     /locus_tag="ACHOT6_000307"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFP9945596.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            34124..35222
                     /gene="prfB"
                     /locus_tag="ACHOT6_000308"
     CDS             join(34124..34198,34200..35222)
                     /gene="prfB"
                     /locus_tag="ACHOT6_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFP9945597.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDVLEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            35232..36749
                     /gene="lysS"
                     /locus_tag="ACHOT6_000309"
     CDS             35232..36749
                     /gene="lysS"
                     /locus_tag="ACHOT6_000309"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFP9945598.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(36792..37340)
                     /gene="idi"
                     /locus_tag="ACHOT6_000310"
     CDS             complement(36792..37340)
                     /gene="idi"
                     /locus_tag="ACHOT6_000310"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFP9945599.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSTFAQHN"
     gene            37395..37466
                     /locus_tag="ACHOT6_000311"
     CDS             37395..37466
                     /locus_tag="ACHOT6_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945600.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(37463..37588)
                     /gene="yqfG"
                     /locus_tag="ACHOT6_000312"
     CDS             complement(37463..37588)
                     /gene="yqfG"
                     /locus_tag="ACHOT6_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFP9945601.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(37590..39038)
                     /gene="uacT"
                     /locus_tag="ACHOT6_000313"
     CDS             complement(37590..39038)
                     /gene="uacT"
                     /locus_tag="ACHOT6_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFP9945602.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLDVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            39474..41393
                     /gene="ygfT"
                     /locus_tag="ACHOT6_000314"
     CDS             39474..41393
                     /gene="ygfT"
                     /locus_tag="ACHOT6_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFP9945603.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPQQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            41398..41871
                     /gene="ygfS"
                     /locus_tag="ACHOT6_000315"
     CDS             41398..41871
                     /gene="ygfS"
                     /locus_tag="ACHOT6_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9945604.1"
                     /translation="MVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQRLDSI
                     SAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMITIQSLP
                     GDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGENPL"
     gene            complement(41907..43274)
                     /gene="ghxQ"
                     /locus_tag="ACHOT6_000316"
                     /gene_synonym="ygfQ"
     CDS             complement(41907..43274)
                     /gene="ghxQ"
                     /locus_tag="ACHOT6_000316"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFP9945605.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(43310..44626)
                     /gene="guaD"
                     /locus_tag="ACHOT6_000317"
     CDS             complement(43310..44626)
                     /gene="guaD"
                     /locus_tag="ACHOT6_000317"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFP9945606.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(44644..46044)
                     /gene="xanQ"
                     /locus_tag="ACHOT6_000318"
     CDS             complement(44644..46044)
                     /gene="xanQ"
                     /locus_tag="ACHOT6_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFP9945607.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(46209..49079)
                     /gene="xdhD"
                     /locus_tag="ACHOT6_000319"
     CDS             complement(46209..49079)
                     /gene="xdhD"
                     /locus_tag="ACHOT6_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFP9945608.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKMLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(49076..49855)
                     /gene="ygfM"
                     /locus_tag="ACHOT6_000320"
     CDS             complement(49076..49855)
                     /gene="ygfM"
                     /locus_tag="ACHOT6_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFP9945609.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAISRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(49906..51234)
                     /gene="ssnA"
                     /locus_tag="ACHOT6_000321"
     CDS             complement(49906..51234)
                     /gene="ssnA"
                     /locus_tag="ACHOT6_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFP9945610.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(51237..54335)
                     /gene="ygfK"
                     /locus_tag="ACHOT6_000322"
     CDS             complement(51237..54335)
                     /gene="ygfK"
                     /locus_tag="ACHOT6_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFP9945611.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(54657..55235)
                     /gene="mocA"
                     /locus_tag="ACHOT6_000323"
     CDS             complement(54657..55235)
                     /gene="mocA"
                     /locus_tag="ACHOT6_000323"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFP9945612.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRCNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            55338..56108
                     /gene="yqeC"
                     /locus_tag="ACHOT6_000324"
     CDS             55338..56108
                     /gene="yqeC"
                     /locus_tag="ACHOT6_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFP9945613.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            56156..57781
                     /gene="yqeB"
                     /locus_tag="ACHOT6_000325"
     CDS             56156..57781
                     /gene="yqeB"
                     /locus_tag="ACHOT6_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFP9945614.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVCADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(57822..58754)
                     /gene="arcC"
                     /locus_tag="ACHOT6_000326"
     CDS             complement(57822..58754)
                     /gene="arcC"
                     /locus_tag="ACHOT6_000326"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9945615.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(58802..60187)
                     /gene="hyuA"
                     /locus_tag="ACHOT6_000327"
     CDS             complement(58802..60187)
                     /gene="hyuA"
                     /locus_tag="ACHOT6_000327"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFP9945616.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(60240..61451)
                     /gene="ygeY"
                     /locus_tag="ACHOT6_000328"
     CDS             complement(60240..61451)
                     /gene="ygeY"
                     /locus_tag="ACHOT6_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFP9945617.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(61509..62705)
                     /gene="dpaL"
                     /locus_tag="ACHOT6_000329"
     CDS             complement(61509..62705)
                     /gene="dpaL"
                     /locus_tag="ACHOT6_000329"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFP9945618.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(62763..63950)
                     /gene="ygeW"
                     /locus_tag="ACHOT6_000330"
     CDS             complement(62763..63950)
                     /gene="ygeW"
                     /locus_tag="ACHOT6_000330"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFP9945619.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            64429..66207
                     /gene="ygeV"
                     /locus_tag="ACHOT6_000331"
     CDS             64429..66207
                     /gene="ygeV"
                     /locus_tag="ACHOT6_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFP9945620.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(66247..66726)
                     /gene="xdhC"
                     /locus_tag="ACHOT6_000332"
     CDS             complement(66247..66726)
                     /gene="xdhC"
                     /locus_tag="ACHOT6_000332"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFP9945621.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(66723..67601)
                     /gene="xdhB"
                     /locus_tag="ACHOT6_000333"
     CDS             complement(66723..67601)
                     /gene="xdhB"
                     /locus_tag="ACHOT6_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFP9945622.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(67612..69909)
                     /gene="xdhA"
                     /locus_tag="ACHOT6_000334"
     CDS             complement(67612..69909)
                     /gene="xdhA"
                     /locus_tag="ACHOT6_000334"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFP9945623.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMDDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            70324..71079
                     /gene="actS"
                     /locus_tag="ACHOT6_000335"
     CDS             70324..71079
                     /gene="actS"
                     /locus_tag="ACHOT6_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFP9945624.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            71158..71231
                     /locus_tag="ACHOT6_000336"
     tRNA            71158..71231
                     /locus_tag="ACHOT6_000336"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:71190..71192,aa:Gly,seq:ccc)
     gene            71465..73075
                     /locus_tag="ACHOT6_000337"
     CDS             71465..73075
                     /locus_tag="ACHOT6_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFP9945625.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            73088..73207
                     /locus_tag="ACHOT6_000338"
                     /pseudo
     CDS             73088..73207
                     /locus_tag="ACHOT6_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            73167..73768
                     /locus_tag="ACHOT6_000339"
                     /pseudo
     CDS             73167..73768
                     /locus_tag="ACHOT6_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT        18023 a        18246 c        19350 g        18149 t
ORIGIN      
        1 tcctgcaata tattgaattt gcaggttttt gtaggccgga taaggcgttc acgccgcatc
       61 cggcatgaca aagcgcacat tgatatctgt ctgagccccg ctctgcgggg attttttcgc
      121 caaaagcaag gtgattcaga tgagaaatct gtgatctatt tggcaaaatt atgctttatt
      181 gtttaccctt gtcagactgc ccgtcataag gcggcggagt gtatttctcc attttgagtc
      241 agttgaaaag gaatattgaa tggaagattt gaatgttgtc gatagcataa acggcgcggg
      301 aagctggctg gtagctaacc aggcgctgct gctaagttat gcagtaaaca tcgtggcggc
      361 actcgcgatc atcatcgttg gtttgattat cgcgcggatg atttccaacg cggtgaatcg
      421 cctgatgatc tcccgtaaaa tcgatgccac tgttgctgat tttctttctg cattagtccg
      481 ttacggtatt atcgccttta cgctaatcgc tgccctggga cgcgtgggtg tacaaacagc
      541 gtcagtcatt gctgtactcg gtgccgcagg cttagctgtt ggtctggctt tgcaggggtc
      601 actttctaac ctggccgctg gcgtgttact tgtcatgttc cgcccgttcc gtgccggaga
      661 atatgttgac ctcggcggcg tagccggtac tgtgctgagt gtgcagattt tctccaccac
      721 catgcgtact gcagacggta aaattatcgt tattccgaac ggtaaaatta ttgccggaaa
      781 tattattaac ttctcccgcg agccagttcg ccgtaacgaa tttattattg gcgtggcgta
      841 tgattccgat atcgatcagg ttaagcagat cctgaccaat attatccagt ctgaagatcg
      901 cattttgaaa gatcgcgaaa tgactgtgcg cctgaacgaa cttggtgcat cgtcgattaa
      961 tttcgtggtc cgcgtctgga gcaacagcgg cgatctgcaa aacgtgtact gggatgtgct
     1021 ggagcgtatt aaacgtgaat ttgatgccgc cggtatcagc ttcccgtacc cgcaaatgga
     1081 tgtgaacttt aagcgggtga aagaagacaa agctgcgtaa tcaacgctga ggccagataa
     1141 tactctctgg cctctctctt attagttatt ctgattgcca attaatatta tcaatttccg
     1201 ctaataacaa tcccgcgata tagtctctgc atcagatact taattcggaa tatccaacgt
     1261 gttttcttat tactttcaag gtcttgctct tggggcagct atgattctgc cgctcggccc
     1321 acaaaatgct tttgtgatga atcagggcat tcgtcgtcag taccacatta tgattgcctt
     1381 actttgcgct atcagcgatt tggtcctgat ttgcgcgggg atttttggtg gtagcgcgtt
     1441 attgatgcag tcgccgtggt tgctggcgct ggtcacctgg ggcggcgtag ccttcttgct
     1501 gtggtatggt tttggcgctt ttaaaacagc gatgagtagt aatattgagt tagctagtgc
     1561 cgaagtcctg aagcaaggca gatggaaaat tatcgccacc atgttggcag tgacctggct
     1621 gaatccgcat gtttacctgg atacttttgt tgtactgggc agccttggcg ggcagcttga
     1681 cgtcgaacca aaacgttggt ttgcgctcgg gacaattagc gcctctttcc tgtggttctt
     1741 tggtctggct attctcgcag cctggctggc accgcgcctg cgcacggcaa aatcacagcg
     1801 cattatcaat ctggtagtgg gatgtgtcat gtggtttata gccttgcagc tggcgagaga
     1861 cggtattgct catgcacaag ccttgttcag ttaggcgcta tctgatggaa aaataaaaca
     1921 gaggcgctaa gcttgcctcc agaggtcctg aattttgggc aagtcaatta acacggagag
     1981 actaacgtga agttcaaagt tatcgccctg gcggcattaa tgggtattag cgggatggca
     2041 gcgcaggcta acgaattgcc ggatggaccg catattgtca cctccggtac ggcaagtgtg
     2101 gatgcggtgc cagacattgc cactcttgcg attgaagtta acgtggccgc gaaggatgcc
     2161 gctacagcca agaaacaggc agatgagcgc gtcgcacaat acatttcctt ccttgaactc
     2221 aatcagatcg cgaaaaaaga tatcagctca gcgaatttac gcacccagcc agattatgat
     2281 tatcaggatg gtaaaagtat cctgaaaggc taccgcgctg tgagaacggt ggaagtcacg
     2341 ctccgtcagt tagacaaact gaattccttg ctggatggcg cgctgaaggc gggtcttaac
     2401 gaaattcgtt ctgtgtcgct gggcgtggcg cagccggatg cctataaaga caaagcgcgt
     2461 aaggcagcga ttgataacgc gattcatcag gcgcaggaac tggcgaacgg ctttcatcgt
     2521 aaactggggc cggtatatag cgtgcgctac catgtttcca actatcagcc cagcccaatg
     2581 gtgcggatga tgaaagccga tgccgcgccg gtgtctgccc aggaaactta cgagcaggcc
     2641 gctattcagt ttgatgatca ggtcgatgtg gtcttccagt tagaacctgt ggatcaacaa
     2701 cccgctaaaa cacctgccgc acaataatac ttgtagcccg acgtattcgt cgggctaatg
     2761 cctttacccg atattgcctc ctttttctgt aattttctgt aaaaccatga cgtaatcacc
     2821 tatctttgct cgcggtttca tcaaggatag gctgactgaa acggttaagc ggacaaggat
     2881 gcgggggagg gggatggaca tttttatttc gaaaaagatg cgcaatttca ttttattagc
     2941 gcaaaccaat aatattgccc gggcggcaga aaaaatccat atgacggctt cgccatttgg
     3001 caaaagcatt gccgcgctgg aagaacaaat tggctatacg ctatttaccc gcaaagataa
     3061 taatatcagc ctcaataaag ccggacagga actgtatcaa aaactgttcc cggtttatca
     3121 acggctttct gcgattgata atgaaatcca taactcaggg cgtcgttcac gagaaattgt
     3181 gattggcata gataatactt atccgacaat tattttcgat cagttgatta gtctcggaga
     3241 taagtatgaa ggcgtgaccg cccagccagt tgagttcagc gaaaacggcg ttatcgataa
     3301 tctttttgac cgtcagttag attttattat ctccccacag catgtatctg cacgagttca
     3361 ggaactggaa aacctgacta tcagtgaatt accaccgctg cgattgggct ttctcgtttc
     3421 tcgccgttat gaagagcgtc aagagcaaga gctgcttcag gagctgccct ggctacaaat
     3481 gcgctttcaa aaccgggcta acttcgaagc aatgattgat gcgaatatgc gaccttgtgg
     3541 gatcaatccc accattattt atcgtcccta cagttttatg gccaaaatca gcgccgtgga
     3601 gcgcggacat tttctgacgg tgatcccgca ttttgcctgg cgactggtga atccggcaac
     3661 gctgaaatat tttgatgcgc cgcacaggcc gatgtatatg caggaatatc tttattcaat
     3721 cagaaatcat cgttataccg ccacgatgct tcagcatatt gctgaagatc gtgacgggac
     3781 aaaccattaa cccagcatcg agccggttgc aattaaatta cggtgtaagt cgaagacgtg
     3841 gctaagatcg tggtgaatat gtccgccggg cgcattttcc agatagcgat gcagataatc
     3901 ccgatacata ggatgtgcac aattatcaat gatagtgcgg gcgcgttgaa gcggggaaag
     3961 accgcgcaga tcggcgatcc cttgttcggt gatgatcact ttgacgctgt gttcgctgtg
     4021 atcaacatgg ctgcacattg gcacgacggt tgagatcttg ccttctttag caatcgacgg
     4081 agccataaag atcgacagat acgcgttgcg ttcaaaatca ccgctgccgc cgatgccgtt
     4141 catcagatcg accccggcta cgtgcgttga atttgcatgc ccgtaaatat caaactccag
     4201 gccgacgttc agagcgatga cgcccagacg acggatgatt tccgggttat tggaaatctc
     4261 ctgcggacgc aacacaatgc ggctggcaaa gtaatccata ttgtcgtaaa tcttgcgcag
     4321 ggaatcggcc gagattgtca ggctggaggc gctggccccg ctgattttgc cggtttccag
     4381 taaatgcacc accgattcct gtagcacttc cgaatacatc atgaacggag gaatttccgg
     4441 gttttccccc agacgcgcca ttaccgcatt attgatattg cccacgccac tttgcagcgg
     4501 cagaaattcc ggcggaatac gcccatgcgc catttcctgc aataagaacg tgaccacgtt
     4561 atcggcaatc tgctggcaca tgggattttg cttatccagc atattaccgg cgtcgggcaa
     4621 gttggtttcc acgacggcga caatcttttt cggatcgatt tgcacatagc gggtaccgac
     4681 gcgatccatt gcatggaaga tcgacacgct attgcgccgc ggtggcgcgc caggaatcac
     4741 aatatctgcc agttctgcaa cgcgcggatc gtgatagtga ttaagttcaa tgatcacttt
     4801 cttcgctcgc agcagccagg tcggcgcatt accgatcccg ctggttaacc agactcgacc
     4861 atccggtgcc agtgccgatg cttcaatgac ggcaacatca atgtcgccga agaaaccgta
     4921 attgaccatt tgcgccactt cgctcaaatg caggtcaacg aaactcaccg cgccctgatt
     4981 gatcttttta cgtaaaccgg acgatgtttg atatggcgca cgccaggaaa cagcatcggc
     5041 gtcagaaagt acatcgtcag cggcggcgct gattgacgcc cccgtcagca ggcgaatttg
     5101 atacggcttt ttggcctcat gctgttcgtt agctctgcgg gcaatcgcgg tgggtagggc
     5161 tttcggcgaa cccgctgggg taaagccgct aaatgccacc atgtcgttat gctggataat
     5221 ttctgccgct tcatcggcgg tcatccttgt ccactgagtt tccattttta ctccttcgtt
     5281 cgcagggatt aatgaccaac gaaattaggt ttacgttttt cgaggaaagc gttcatccct
     5341 tcctggtaat cttcgctgtc ataaaccgcg cggcgcatcc cctgaatacg ttcaaattca
     5401 tcggagttca tggtgtgtgc ttcgcccagt acacgcagct cttctttgat aacggcaatg
     5461 gctaacggcg ctttctcaga gatgtggtgc gccatttgta aggtgaaatc ttccagttct
     5521 tccacttcca caacatggtt gaggatgccg acagccagcg cgcgctgggc ggtgattggc
     5581 gaagcggtaa aaatcagctc tttgacaatg tggaagcccg cgtcgcgggt caggttgtga
     5641 atgccgacca ggttatacgg gacgccgagg tttacaggcg tcattgagaa ggttgaggta
     5701 ctggcggcga tgatcagatc ggaactcatg atcatttcaa atgcgccacc ccaaacacta
     5761 ccttccacca tcgaaatgat cggtttcggg aatttttgga tcatgcgggt gatttgacgc
     5821 aatggatcat cataggagag cggatcgcga ccgccagacg gcagttcgtg aatatcgtga
     5881 cctgcggaga agactttgga tccactcggc gcgcgcaaaa tgatacagcg aatttccggc
     5941 cggttgagat cgcttaacgc ctgcataaga tcatcaataa agactttact taaggcatta
     6001 agtttgcggc catagttaaa ctcaacgacc gccactttgt tgatagtgac aacgttaaca
     6061 tactgataag acataaaaat tcctttaatc aaaatattgc gtctggataa attcactgag
     6121 ctgccgcagg ccggtgcgcg gtgagagcgt attgtttttg accgctaaaa gcgtctggcg
     6181 gtaatagcga tcgaaatctt cattcgcgaa caggtgattc agtacttctt cttcggtctg
     6241 cttacgcagc cattccaccg attgttgttg ccgcacttgt tgtaaacgac cactggcagt
     6301 tagcgcggtt ttgaagtcga tgatggcgtg ccagatctca tcgattccac gtttttccag
     6361 tgcgctacaa gtcagaaccc gtggctgcca ttcgtcgtat ttacgtcgca gaatatgcag
     6421 ggcactctcg tacatatgcc gggcaatggc gacattggta tggttatcgc catcgtcttt
     6481 gttgataacg atcagatcag ccacttccat cagccctttt ttaatgccct gcagatcatc
     6541 gccgccaccg gcaatttgca acgagataaa acagtccacc atgcgggcga cttctgtttc
     6601 cgactgcccg acgccaaccg tttcgacaat cactacgtca taacccgctg cttcgcataa
     6661 cagcattaat tcccgcgctc gctgactggc accgcccaga tgaccggagg atggtaccgg
     6721 gcgaataaac gccgcttcgg cacgcgccag gtcattcatg cgggttttat ccccgagaat
     6781 gctaccgcca gtgaccgggc tgctgggatc gaccgcaata accgcgacct ttaatccctc
     6841 tcgaatcaac aacatgccaa aggcctcaag aaaggtactt ttccccgcgc cgggggtgcc
     6901 ggtaacgccc agtcgcaggg tgttaccgca gtacggcata atggcatcaa gcagttgcgt
     6961 acttagcgcc tgatgacgcg ggtgacggct ttccaccagc gtcatggcct gggcgagtgt
     7021 ggcacgctca ccctgacgta agcggcgaat actttctgcc agcgtggctt cattaatcat
     7081 gatgctggct tatcagattc agtacgtcgc gcacactgtc gagcataggt gtacctggac
     7141 cataaatcgc cgccacgccg cgctcttgca ggaaggcgta atcctgcggt ggaatgacgc
     7201 caccggcgac tacgcagata tcttcgcgtc cccatttttt cagcgcttcg accagttccg
     7261 ggatcagcgt tttatgaccg gcagccagtg aggatgcgcc cactacgtga acgtcatttt
     7321 ctactgccag gcgggcgatc tcttcaggtg tagagaacat cgggcttaaa tctacgtcga
     7381 aaccgagatc ggaataggcg ctggcgatca ctttcgcgcc gcgatcgtgc ccatcctggc
     7441 ccattttggc gatcagaatg cgcgggcgac gaccattgtc ggcaaggaac tgctccgttt
     7501 gcgcaacaat ggcatcgaac tcggaggccg atttctcaga ttgatgatag ctttgcgcaa
     7561 tcacgccggt aacacactgg cttggcacca gataacggtc gaaagcggct tccagcgcat
     7621 cggaaatttc acccagggtg gcgcgaacgc gagcggcatt aacagcggca gccagcaggt
     7681 tttcgttatg ctgtgcggcg tgagtcagga cgttcaacgc ggcggttacg gcggcattat
     7741 cacgggtggc gcgaatgcgt tccagcgaag caatttgctc gttacgcacc atcacgttgt
     7801 cgatctcaag tacatcggtt tcgtcttcgt gatccagttt gtacttgttg acaccaacga
     7861 tgacacgctt gccctggtcg atcagcgact gttcgcgcgc tgaggcctct tcgatcattc
     7921 gttttggcag acctgcttcg atcgctttcg ccatgccacc ggcttcgtcg atctgttgga
     7981 taatagctct ggcttgtttg acgatttgat cggtcagcga ctcaatgtaa taggatccgg
     8041 ccagtggatc gacggtgcgg cagagttctg attcttcctg gatgatgatc tgggtgttgc
     8101 gggcaatgcg tgctgagaaa tcggtaggca aaccaagcgc ttcgtcaaag gcgttggtat
     8161 gcagtgactg agtaccgccc agcgtcgcag ccagtgcttc aatggtggtg cggataacgt
     8221 tgttatacgg atcctgttca gtcaggctcc agcctgaggt ctggcagtgg gtacgcagcg
     8281 ccagtgattt cggatcctgt gcgccaaatc cactgaccgc ttcgctccat aaataacgtg
     8341 ccgcacgcaa catggcgacg ttcataaaca gatccatgcc gataccgaag aagaacgaca
     8401 ggcgaggagc gaagtcatca attttcagtc cggcagagat tgctgctttg atgtactcaa
     8461 tcccatcagc gagcgtaaat gctacctgct gcacgcagtt ggcacccgct tcccccatgt
     8521 ggtaaccgct gatactgatg gtattaaatc gcggcatgtt gccggaacac caggcgatga
     8581 tgtcggcgat aatgcgcatt gacggttttg gtgggtaaat ataggtgttg cggcagaggt
     8641 actctttgag aatatcgttt tgaatggtgc cggtcagttt atcaggtgta acaccttgct
     8701 cttctgcggc gacgatataa aacgccagta ctggtagcac tgcgccattc atggtcatcg
     8761 aaaccgacat tttatccagc gggatctggt cgaacaggac tttcatatct tccacggtgt
     8821 cgatagcgac gcccgctttg ccgacgtcac ccgccacgcg tgggttatcg gagtcgtagc
     8881 cacggtgggt ggcaaggtca aacgcaacgg aaagaccttt ttgcccggcg gccaggttac
     8941 ggcgataaaa agcgttggac tcttttgctg ttgaaaaacc agcatactga cggatggtcc
     9001 acggttgggc ggtatacata gtggcacgcg ggccacgaac gtagggcggc aaaccaggaa
     9061 gggtacctgt cacctccaga ttatcgagat cggcttcggt atacagcggc ttgatggcga
     9121 tcccttccgc ggtttgctga accagcgagt cgacagtttt ctcccgacgg ctcaattcct
     9181 tgttggcaag ctgttgccac tcctgctcgt tagacattat ttgctccgta aaacgcaatc
     9241 aaaatgaggg tatttgcgct tacggtgagc tttttacgtg ggtttgtcgc cagcaaaaaa
     9301 atgcattcag acgtcgtttt ttgccgcctg agatttgtct attttgtgat gtgattcaag
     9361 gaatacaaaa agaagtgagg ataacgcctg atatgtgcta tcaggcatta tttgatggat
     9421 taatcctgac gaaggacttt gtgaccataa tcgagcaacg catcagtgac tttacgcatc
     9481 atgcggcttt caggagcaaa gcggtgccag tagagcatcc ggcgttgaaa tagccctggc
     9541 gttaagtcaa tcagttcacc gctggctagt tctttctcga tttgcaggtg cgggatcata
     9601 cagcaggtgg tgccctggcg agcaagttgc acgaacgctt ctgaagaatt aacgatatgg
     9661 cagggcacgc tgcctggagg cagatcgaag ttttgctgca aaaaggcctg gtgcatatcg
     9721 tcaagatggt caaaggcgac cacaggtgct ttcaataatg ccgaacgcgt tacgccgtta
     9781 gggaaatatt tttcggcaaa cggttttgag ctgacgaaca gatagtcaag cgcaccgagc
     9841 ttatcgacaa ggcaactcgg cagcgcctga tgttgaatac tcaccgcgcc gaccacttcg
     9901 ccgcggcgca gacgttcctg agtacgggtt tcatcttcta cctgcaagtt gaggcggata
     9961 ggcgaatcag ccaacacagg agccagtgcc ggaagcaacc acgtcgctag actgtcggcg
    10021 ttgaccgcca gtgaaagcag cagcggagtc gaaccggttt gttcatcgcc cagccactct
    10081 tcttccagca actccacctg gcgcagcagt gccagcagtt tttgcccttg ttccgtcggg
    10141 cgcggcggta cggtacgcac caacagcggc tgcccgaaca tattttccag ttgcttaatg
    10201 cgctgtgaga cggctgattg tgtaatgcac agcttttgtg ccgcgcgctc aaatcctcgt
    10261 tcacgtatca ccgcatccag tgcctgtaat gttctgtagt ccgggcgttt cattgctgtt
    10321 gtctgctcct ggtaaatgct ttttcctgca ctatgacaca attttgtgtc aggttgcata
    10381 taccgcagcc gtcggcgggt aacatttgta tcaatggtta aacgcgcagc ttaaaagatc
    10441 agggatttgc gctattgcat ttcgcttagc gacttcgcgc cctggttatg ttgaatggcg
    10501 tggcgttatt gcctctgttc gcaccgtgga gcggcatacc cagaccgttg tatgctttat
    10561 ttgcggggcg aaaggggatg cccgccattg cgcgatattt tatggatgag ttaaccacgc
    10621 ggcttgccaa cggggtctga atcgcttttt ttgtatataa tgcgtgtgaa atttcatacc
    10681 acaggcgaaa cgatcatgac gcaggatgaa ttgaaaaaag cagtaggatg ggcggcactt
    10741 cagtatgttc agcccggcac cattgttggt gtaggtacag gttccaccgc cgcacacttt
    10801 attgacgcgc tcggtacaat gaaaggccag attgaagggg ccgtttccag ttcagatgct
    10861 tccactgaaa aactgaaaag cctcggcatt cacgtttttg atctcaacga agtcgacagc
    10921 cttggcatct acgttgatgg cgcagatgaa atcaacggcc acatgcaaat gatcaaaggc
    10981 ggcggcgcgg cgctgacccg tgaaaaaatc attgcttcgg ttgcagaaaa atttatctgt
    11041 attgcagacg cttccaagca ggttgatatt ctgggtaaat tcccgctgcc agtagaagtt
    11101 atcccgatgg cacgtagtgc agtggcgcgt cagctggtga aactgggcgg tcgtccggaa
    11161 taccgtcagg gcgtggtgac cgataatggc aacgtgatcc tcgacgtcca cggcatggaa
    11221 atccttgacc cgatagcgat ggaaaacgcc ataaatgcga ttcctggcgt ggtgactgtt
    11281 ggcttgtttg ctaaccgtgg cgcggacgtt gcgctgattg gcacacctga cggtgtcaaa
    11341 aacattgtga aatgatctga cgggggaacc tcccccgtta aaaaaattct cttcattaaa
    11401 tttggtgaca tgtgtcacgc ttttaccagg caattgtcga ttgctctaaa taaatcctct
    11461 aaaccagcat attcatccaa gaattacctt tgcgtgatat ttcctcaaca tcgcgacgca
    11521 aacgttcata ttgccgcaat attatttttt gatatgttga aaggcggatg caaatccgca
    11581 cacaacattt caaaagacag gattgggtaa atggcaaagg tatcgctgga gaaagacaag
    11641 attaagtttc tgctggtaga aggcgtgcac caaaaggcgc tggaaagcct tcgtgcagct
    11701 ggttacacca acatcgaatt tcacaaaggc gcgctggatg atgaacaatt aaaagaatcc
    11761 atccgcgatg cccacttcat cggcctgcga tcccgtaccc atctgactga agacgtgatc
    11821 aacgccgcag aaaaactggt cgctattggc tgtttctgta tcggaacaaa ccaggttgat
    11881 ctggatgcgg cggcaaagcg cgggatcccg gtatttaacg caccgttctc aaatacgcgc
    11941 tctgttgcgg agctggtgat tggcgaactg ctgctgctat tgcgcggcgt gccggaagcc
    12001 aatgctaaag cgcaccgtgg cgtgtggaac aaactggcgg cgggttcttt tgaagcgcgc
    12061 ggcaaaaagc tgggtatcat cggctacggt catattggta cgcaattggg cattctggct
    12121 gaatcgctgg gaatgtatgt ttacttttat gatattgaaa acaaactgcc gctgggcaac
    12181 gccactcagg tacagcatct ttctgacctg ctgaatatga gcgatgtggt gagtctgcat
    12241 gtaccagaga atccgtccac caaaaatatg atgggcgcga aagagatttc gctaatgaag
    12301 cccggctcgc tgctgattaa tgcctcgcgc ggtactgtgg tggatattcc ggcgctgtgt
    12361 gatgcgctgg cgagcaaaca tctggcgggg gcggcaatcg acgtattccc gacggaaccg
    12421 gcgaccaata gcgatccatt tacctctcca ctgtgtgaat tcgacaacgt ccttctgacg
    12481 ccacacattg gcggttcgac tcaggaagcg caggagaata tcggcctgga agttgcgggt
    12541 aaattgatca agtattctga caatggctca acgctctctg cggtgaactt cccggaagtc
    12601 tcgctgccac tgcatggtgg tcgtcgtctg atgcacatcc acgaaaaccg tccgggcgtg
    12661 ctaactgcgc tgaacaaaat cttcgccgag cagggcgtca acatcgccgc gcaatatcta
    12721 caaacttccg cccagatggg ttatgtagtt attgatattg aagctgacga agacgttgcc
    12781 gaaaaagcgc tgcaggcaat gaaagctatt ccgggtacca ttcgcgcccg tctgctgtac
    12841 taattcccct tctctgaaaa ttaacgggca ggtcactgac ttgcccgttt ttttatccct
    12901 tctccacacc gttgccgcgt tgcgcattct actttgcgag tctgctccag aaaataaatc
    12961 aaagtcagat caatgcgttg tgtttattta atggggattt acgatggcgg gacagcatgg
    13021 ggctgtaacg gtaaagccct caccgaagcg agggcttaaa ggagaagggt tatgatgcga
    13081 cttgtcatca tactgattgt actgttactg ataagtttcg gagcttatta acagtcagtc
    13141 tcaggggagg agtcatcctc ccttaccctt actcactaaa ttaggtcaaa gaatcaacga
    13201 tgtcaatcag ggcgatgcgg ggtgcatcgc cctgaaccga actttttgat agccacttca
    13261 tcgcccacgt ttactgccgt aatagatcct ttttcccaaa aattatctct gttttgcatg
    13321 gggtttttcg agcacgctcg aagaaaagat ttaaaggtag atcaatgcgt tgtggttatt
    13381 taaagagagc ttacgatagc tgggcagcat ggggctgtaa cggtaaagcc ctcaccgaag
    13441 cgagggcttg aaggagaagg gttatgatgc gacttgccat catactgatt gtactgttac
    13501 tcataagttt cccagcttat taacagtcag tcacagggga ggagcaatcc tcccttaccc
    13561 ttactcacta aattaggtta aagaatcaac gatgtcaatc agggcgatgc aggtgtatcg
    13621 cccttaccac tcccagactt tcgacggtgt aaccaccgca ggaagaggga tatcccactc
    13681 ttcaacgggg agtttttcca ccaactgaca atcatgcgca taacccaccg gttgcgtttt
    13741 atagtgctgc cagttttgta aggtccgatc ataaaaacca ccgcccattc ccaggcgctg
    13801 accgtactca tcaaaggcga ccagcggtgt gatcagcacg tctaatcggg aaaggggtag
    13861 cacgtcacgc acatccagtt ttggctcatg gatcttcaac ctgttcatca ccagttcgct
    13921 ttgcggatgg taattcagga acagcaaatt accggcacta aagggatgca aaactggaag
    13981 atatacgcgc ttaccggcgc gccagagttg ttctattagc ggctgggtgt cgagttcacc
    14041 atcaaaagag aggaatacag cgaccgtatg cgccatcacc accgggggat aagtcatcat
    14101 ccgggtagcg gcttgttgac ccatttcctg ctgttgttcc ggcgttaacg cacgacgacg
    14161 ttgccgaatc attttgcgga tttcttgtcg ggataatgtc agtgggagtt ctggtagttg
    14221 cgtcatggct ggtgccagat aagaagggaa tctccgagat gccgccgcag gctgtaaccc
    14281 ttgaaccctt ggttcaaggt gaatgtgtcg tcgcagtttt aaggcttctc ggacggaccg
    14341 agcatgctca ccaaccgcgg agcgccacat tcttgtggta tgaaatatcg gctcagggga
    14401 ctggcccgct tgcgaacatc tcagagaaat tttgtcttca cggttactct accacagtaa
    14461 accgaaaagt gtcattcaaa gttttggtta gttttttcgg tgatgcgacc ttgttcaagt
    14521 aacgcttgtt ctatggtctg ctgcagcatc cgaatacgct gttccatact tgccgcgtag
    14581 tcacgagtct tcgctttttc ttgcgctaac tcatagctga tattcaatgc ggcaatgaag
    14641 accaactgtt cagtatttgt gactctagtg cgttctttca gatcttgcaa ccgttggttc
    14701 agatcgtccg ctgcctgatt caacgcatcc ctttggtcag gcgggcagtt cacacgcagt
    14761 gaacggccaa aaatttggat atcgacgggt tgtgcagaca tgccaccttc ctgctgattg
    14821 actgcgctgc cttcgtcttt agacccatgg tctgcgaagg ggcgacacta tagctaccct
    14881 gatgagaaga gacaagccct tttctggtcc accagggccc aaagtggtag catatcatga
    14941 atattcctcc ctttgacgac gaatgcttat gtctatacag aacgaaatgc ctggttacaa
    15001 cgaaatgaac cagtatctga accaacaagg gacgggtctg acccctgctg agatgcatgg
    15061 tttaatcagt gggatgatat gtggcggtaa cgatgacagc tcatggctac cgctacttca
    15121 cgacctgacg aacgaaggca tggctttcgg tcatgagctg gcacaggcac tgcgtaaaat
    15181 gcactctgcc accagcgatg ccctgcagga tgacggcttc ctttttcagc tttatctgcc
    15241 tgatggcgat gatgtcagcg ttttcgatcg ggctgatgca ttggcaggtt gggtcaatca
    15301 cttcctgctt ggtcttggcg ttacgcaacc gaagctggat aaagtgaccg gcgaaaccgg
    15361 tgaagctatc gacgatctgc gtaacattgc gcaactgggt tacgacgaag acgaagatca
    15421 ggaagagctt gaaatgtcgc ttgaagagat catcgaatac gttcgtgttg ccgcgctgtt
    15481 atgccacgac acctttactc atccgcaacc gaccgcgcca gaagtacaaa aaccgactct
    15541 acactaaaaa caaaaaacgt aaggagagtg ttatgagtga gatatcccgg caagagtttc
    15601 agcgtcgccg tcaggccctg gtggagcaaa tgcaacccgg cagcgccgcg ctgatttttg
    15661 ctgcaccaga agtaacacgt agcgccgaca gcgaataccc ctatcgtcag aacagtgact
    15721 tctggtactt caccggcttt aacgaaccgg aagcggtgct ggtgctgatt aaaagcgatg
    15781 acactcataa ccacagcgtt ctgtttaacc gcgttcgcga cctgacggcg gagatctggt
    15841 ttggccgtcg cttaggccag gatgccgcgc cagagaaact gggcgttgac cgcgcactgg
    15901 cattcagcga aatcaatcag caactttatc aactacttaa cggcctggat gtggtttacc
    15961 atgcccaggg cgaatatgca tatgctgatg aaatcgtgaa cagtgcgctg gaaaaactgc
    16021 gtaaaggttc gcggcaaaat ctcaccgcac cggcaacgat gatcgactgg cgtcctgttg
    16081 ttcatgaaat gcgcctgttc aaatcgccag aagagattgc cgtactccgc cgcgcgggag
    16141 aaatcaccgc catggcacat acccgggcga tggaaaaatg ccgtccggga atgttcgagt
    16201 accatctgga aggcgaaatt caccacgaat ttaaccgcca cggtgcgcgc tatccgtcct
    16261 ataacaccat tgtcggcagc ggtgaaaacg gctgcattct gcactacacc gaaaacgagt
    16321 gtgaaatgcg cgacggcgac ctggtgttga ttgacgcggg ttgcgaatac aaaggttacg
    16381 ctggcgatat tacccgcacc ttcccggtca acggcaaatt cactcaggcc cagcgtgaaa
    16441 tctacgacat tgtgctggag tctctcgaaa ccagcttgcg cctgtatcgt ccgggaactt
    16501 ccattctgga agtcactggt gaagtggtgc gcatcatggt tagcggcctg gtaaaactcg
    16561 gcattctgaa aggtgatgtt gatgaactga tcgctcagaa cgcccatcgt cctttcttta
    16621 tgcatggcct tagccactgg ttagggctag atgtccatga cgttggcgtt tatggtcagg
    16681 atcgctcgcg cattctggaa ccggggatgg tactgaccgt agagcctggt ctgtatatcg
    16741 cgccggatgc ggatgtgcca gaacaatatc gcggtatcgg cattcgtatt gaagacgaca
    16801 ttgtgattac cgaaaccggc aacgaaaacc tcaccgccag cgtggtgaaa aagccggaag
    16861 aaatcgaagc gttgatggca gctgcgagaa agcaatgagc gtaatcatcg tcggtggcgg
    16921 catggcgggc gcgacgctgg cgctggctat ttcccggtta agtcacgggg cgctgccggt
    16981 acatttgatt gaagcgaccg cgccagagtc acatgctcat ccgggctttg atggacgagc
    17041 tattgcgctg gctgcgggga cctgtcagca actggcgcgc atcggcgtct ggcaatctct
    17101 ggcggattac gcaaccgcca tcactaccgt gcatgtcagc gatcgtggtc acgctggatt
    17161 tgtcaccctc gccgcagaag attaccaact ggcggcgctg ggacaggttg tcgaattgca
    17221 caatgtcggg caacggctgt ttgcattgct gcgtaaagca cctggcgtaa cgctgcattg
    17281 ccctgatcgc gtggctaacg ttgcccgtac tcagagtcac gttgaagtga cgctggagag
    17341 tggcgagacg ctgacgggcc gcgtgctggt agcagctgat ggcacccatt cagcgttagc
    17401 caccgcgtgc ggcgttgact ggcagcagga gccttacgaa caactggccg tgattgccaa
    17461 cgttgcgact tccgttgcgc acgaaggccg cgcttttgaa cgcttcacgc aacatggccc
    17521 gctggcgatg ttgccgatgt ctgacggacg ctgttcgctg gtctggtgtc atccactgga
    17581 acggcgcgaa gaggtgctgt cgtggagcga cgagaagttt tgccgtgaac tccagtcggc
    17641 ctttggctgg cgacttggaa aaattaccca cgccggtaaa cgcagtgctt atccgctggc
    17701 gttaacccgc gccgccagag ctattaccca tcgtaccgtg ctggtgggca atgcggcgca
    17761 aactctgcac ccgattgccg ggcaagggtt taacctcggt atgcgagatg tcatgagcct
    17821 tgcggaaacc ctgactcagg cgcatgagcg cggagaagac atcggtgatt acggcatatt
    17881 gtgccgctat cagcagcgtc gacagagtga tcgcgaagca accatcggcg tcacggacag
    17941 cctggtacat ctttttgcca accgttgggc accgctggtt gtcgggcgca acatcggact
    18001 gatgacgatg gaattattca ccccggcacg cgatgtgctg gcgcagcgca ccctcggttg
    18061 ggtggcgcgt tgaaggcggt tttaaggaat aaacaatgca aagtgttgat gtagccattg
    18121 ttggtggcgg catggtgggg ctggcggttg cctgcggctt acaggggagc ggcttacgcg
    18181 ttgccgtact ggagcagcgc gtaccggaac ctctggcggc ggatgcacca ccacaactgc
    18241 gcgtttcggc tatcaatgcc gccagcgaaa aattactcac ccgtcttggc gtctggcagg
    18301 acattctctc tcgtagggcc agttgttatc acggtatgga agtgtgggac aaagacagtt
    18361 ttggtcacat ttcgtttgac gatcaaagca tgggctatag ccatcttggg catatcgttg
    18421 aaaactcagt gattcactac gcgctgtgga acaaagcgca gcagtcgtca gatatcactc
    18481 tgttggcccc cgcagaatta cagcaggtcg cctggggaga aaatgaaact ttcctgacgc
    18541 tgaaagatgg cagcatgtta acggcgcgtc tggtgattgg cgcggacggt gctaattcct
    18601 ggttgcgcaa caaagcagat attccgctga ctttctggga ttatcagcat cacgcgctgg
    18661 tagcgaccat tcgcacggaa gaaccgcatg atgcggtggc gcggcaggtt ttccatggcg
    18721 aaggcattct ggccttttta ccgcttagcg atccgcatct ttgctcaatt gtctggtcac
    18781 tgtcgccaga ggaagcgcag cggatgcagc aggcaagtga agatgaattt aatcgcgcgt
    18841 taaatatcgc ttttgataat cgcctgggct tatgcaaggt tgagagcgcg cgtcaggtgt
    18901 tcccactgac ggggcgttat gcgcgccagt ttgccgcgca ccgtctggtg ctggtgggcg
    18961 acgccgcgca taccattcac ccgctggcgg ggcagggagt aaatctcggc tttatggatg
    19021 ctgcagagct gattgctgaa ctgaaacggt tgcatcgtca ggggaaagac atcgggcagt
    19081 acatttacct gcgccggtat gagcgtagcc gcaagcacag tgcggcgctg atgctggctg
    19141 gtatgcaggg attccgcgat ctgttttccg gtaccaatcc ggcgaaaaaa ctgctgcgtg
    19201 atattggttt gaaactggcc gacacgcttc ctggcgttaa accacaactt atccgccagg
    19261 caatgggatt aaacgatttg cctgaatggc tgcgttaaaa atttctcttc tgctgtttat
    19321 ttgataccca tcacactttc atctcccggt tttttcgccg ggagattttc ctcatttgaa
    19381 ataaactaat ttcacctccg ttttcgcatt atattttcta atgtcattat tttttgattt
    19441 agtgtttttt gacatttttt tagctcttaa tattgcctta ttcaaattag ctttctaatg
    19501 gcatcatctt tatatagaaa ctggtgtgtt ttttggtttt ttattctgtc gcgattttgg
    19561 cgttttttaa ccataagcta atgtgatgat caattttacc ttatggttaa cagtctgttt
    19621 cggtggtaag ttcaggcaaa agagaacgat tgcgttgggg accgggagtg gctccgatgc
    19681 tgggtttcgt ggtgataatt tcaccatgaa aagttgtcag ccccgcttat tcaatgagga
    19741 caagatggca caacagactc ctttgtacga acaacacacg ctttgcggcg ctcgcatggt
    19801 ggatttccac ggctggatga tgccgctgca ttacggttcg caaatcgacg aacatcatgc
    19861 ggtacgtacc gatgccggaa tgtttgatgt gtcacatatg accatcgtcg atctccgcgg
    19921 cagccgcacc cgggagtttc tgcgttatct gctggcgaac gatgtggcga agctcaccaa
    19981 aagcggcaaa gccctttact cggggatgtt gaatgcctct ggcggtgtga tagatgacct
    20041 catcgtctac tactttactg aagatttctt ccgcctcgtt gttaactccg ccacccgcga
    20101 aaaagacctc tcctggatta cccaacacgc tgaacctttc ggcatcgaaa ttaccgttcg
    20161 tgatgacctt tccatgattg ccgtgcaagg gccgaatgcg caggcaaaag ctgccacact
    20221 gtttaatgac gcccagcgtc aggcggtaga agggatgaaa ccgttctttg gcgtgcaggc
    20281 gggcgatctg tttattgcca ccactggtta taccggtgaa gcgggctatg aaattgcgct
    20341 gcccaatgaa aaagcggccg atttctggcg tgcgctggtg gaagcgggcg ttaagccatg
    20401 cggtctgggc gcgcgtgaca cgctgcgtct ggaagcgggc atgaatcttt atggtcagga
    20461 gatggacgaa accatctctc cgttagccgc caacatgggc tggactatcg cctgggaacc
    20521 ggcagatcgt gacttcatcg gtcgtgaagc cctggaagtg cagcgtgagc atggcacaga
    20581 aaaactggtt ggtctggtga tgaccgaaaa aggcgtgctg cgtaatgaac tgccggtacg
    20641 ttttaccgat gcgcagggca accagcatga aggcattatc accagcggga ctttctcccc
    20701 gacgctgggt tacagcattg cgctggcacg cgtgccggaa ggtattggcg aaacggcgat
    20761 tgtgcaaatt cgcaaccgtg aaatgccggt taaagtgacg aaacctgttt ttgtgcgtaa
    20821 cggtaaagcc gtcgcgtgat ttactttttt ggagattgat tgatgagcaa cgtaccagca
    20881 gaactgaaat acagcaaaga acacgaatgg ctgcgtaaag aagccgacgg cacttacacc
    20941 gttggtataa ccgaacatgc ccaggagctg ttaggcgata tggtgtttgt tgacctgccg
    21001 gaagtgggcg caacggttag cgcgggcgat gactgcgcgg ttgccgaatc agtaaaagcg
    21061 gcgtcagaca tttatgcgcc agtaagcggt gaaatcgtgg cggtaaacga cgcactgagc
    21121 gattccccgg aactggtgaa cagcgaaccg tatgcaggtg gctggatctt taaaatcaaa
    21181 gccagcgatg aaagcgaact ggaatcactg ctggatgcga ccgcatacga agcattgtta
    21241 gaagacgagt aacggcttta ttcctcttct gcgggagagg atcagggtga ggaaaattta
    21301 tgcctcaccc tcactctctt cgtaaggaga gaggttcaca attcactgca cgtttcagga
    21361 accatcgctc atgacacaga cgttaagcca gcttgaaaac agcggcgctt ttattgaacg
    21421 ccatatcgga ccggacgccg cgcaacagca agaaatgctg aatgccgttg gtgcacaatc
    21481 gttaaacgcg ctgaccggcc agattgtgcc gaaagatatt cagcttgcga ccccgccgca
    21541 ggttggcgca ccggcgaccg aatacgccgc actggcagaa ctcaaggcta ttgccagtcg
    21601 caataaacgc ttcacgtctt acatcggcat gggttacacc gccgtgcagc taccgccggt
    21661 tatcctgcgt aacatgctgg aaaatccggg ctggtatacc gcgtacactc cgtatcaacc
    21721 tgaagtctcc cagggccgcc ttgaagcact gctcaacttc cagcaggtaa cgctggattt
    21781 gactggactg gatatggcct ctgcttctct tctggacgag gccaccgctg ccgccgaagc
    21841 aatggcgatg gcgaaacgcg tcagcaaact gaaaaatgcc aaccgcttct tcgtggcttc
    21901 cgatgtgcat ccgcaaacgc tggatgtggt ccgtactcgt gccgaaacct ttggttttga
    21961 agtgattgtc gatgacgcgc aaaaagtgct cgaccatcag gacgtcttcg gcgtgctgtt
    22021 acagcaggta ggcactaccg gtgaaattca cgactacact gcgcttatta gcgaactgaa
    22081 atcacgcaaa attgtggtca gcgttgccgc cgatattatg gcgctggtgc tgttaactgc
    22141 gccgggtaaa cagggcgcgg atattgtttt tggttcggcg caacgcttcg gcgtgccgat
    22201 gggctacggt ggcccacacg cggcattctt tgcggcgaaa gatgaataca aacgctcaat
    22261 gccgggccgt attatcggtg tatcgaaaga tgcagctggc aataccgcgc tgcgcatggc
    22321 gatgcagact cgcgagcaac atatccgccg tgagaaagcg aactccaaca tttgtacttc
    22381 ccaggtactg ctggcaaaca tcgccagcct gtatgccgtt tatcacggcc cggttggcct
    22441 gaaacgtatc gctaaccgca ttcaccgttt gaccgatatc ctggcggcag gcctgcaaca
    22501 aaaaggtctg aagctgcgcc atgcgcacta tttcgacact ctgtgtgtgg aagtggccga
    22561 caaggcgggc gtactggcgc gtgccgaagc ggctgaaatc aacctgcgta gcgatatcct
    22621 gaacgcggtt gggatcaccc ttgatgaaac caccacgcgt gaaaacgtaa tgcagctttt
    22681 cagcgtgctg ctgggcgata accacgggct ggagatcgac acgctggaca aagacgtggc
    22741 tcacgacagc cgctctattc agcctgcgat gctgcgcgac gacgaaatcc tcactcatcc
    22801 ggtgtttaat cgctaccaca gcgaaaccga aatgatgcgc tatatgcact cgctggagcg
    22861 taaagatctg gcgctgaatc aggcgatgat cccgctgggt tcctgcacca tgaaactgaa
    22921 cgccgccgcc gagatgatcc caatcacctg gccggaattt gccgaactgc acccgttttg
    22981 cccgccggaa caggctgaag gttatcagca gatgattgcg cagctggctg actggctggt
    23041 gaaactgacc ggttacgacg ccgtttgtat gcagccgaac tccggcgcac agggcgaata
    23101 cgcgggcctg ctggcgattc gtcattatca tgaaagccgc aacgaagggc atcgcgatat
    23161 ctgcctgatc ccggcttctg cgcacggaac taaccccgct tctgcacata tggcaggaat
    23221 gcaggtggtg gttgtggcgt gtgataaaaa cggcaacatc gatctgactg atctgcgcgc
    23281 gaaagcggaa caggcgggcg ataacctctc ctgtatcatg gtgacttatc cttctaccca
    23341 cggcgtgtat gaagaaacga tccgtgaagt gtgtgaagtc gtgcatcagt tcggcggtca
    23401 ggtttacctt gatggcgcga acatgaacgc ccaggttggc atcacctcgc cgggctttat
    23461 tggcgcggac gtttcgcacc tcaacctgca taaaactttc tgcattccgc acggcggtgg
    23521 tggtccgggt atgggaccga tcggtgtgaa agcgcatctg gcaccgtttg taccgggtca
    23581 tagcgtggtg caaatcgaag gcatgttaac ccgtcagggc gcagtttctg cggcaccgtt
    23641 cggtagcgcc tctatcctgc caatcagctg gatgtacatc cgcatgatgg gcgcagaagg
    23701 gctgaaaaaa gcaagccagg tggcaatcct caacgccaac tatattgcca gccgcctgca
    23761 ggatgccttc ccggtgctgt ataccggtcg cgacggtcgc gtggcgcacg aatgtattct
    23821 cgatattcgc ccgctgaaag aagaaaccgg catcagcgag ctggatattg ccaagcgcct
    23881 gatcgactac ggtttccacg cgccaacgat gtcgttcccg gtggcgggta cgctgatggt
    23941 tgaaccgact gaatctgaaa gcaaagtgga actggatcgc tttatcgacg cgatgctggc
    24001 tatccgcgca gaaattgacc aggtgaaagc cggtgtctgg ccgctggaag ataacccgct
    24061 ggtgaacgcg ccgcacattc agagcgaact ggtcgccgag tgggcgcatc cgtacagccg
    24121 tgaagttgcg gtattcccgg caggcgtggc agacaaatac tggccgacgg tgaaacgtct
    24181 ggatgatgtt tacggcgacc gtaacctgtt ctgctcctgc gtaccgatta gcgaatacca
    24241 gtaattcact gattcgacta ttttctaaag gcgcttcggc gcctttttag tcagatgaca
    24301 aagtacaaaa gtgctcagac agtcccctcg cccctttggg gagagggtga ggggaacagg
    24361 ccggcactgg cgcgaatatt taccctcacc ccggccctct ccctgaaagg gcgaggggga
    24421 aaagcgtgcc aacattgaat attgagccag tttgttagca atctcaaaga tacgtcaacg
    24481 aatgaatttt tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc
    24541 atcgggcggg caactgcatt actgttggcg caagaagggt atacggtggc ggttaattat
    24601 caacaaaacc tccacgcggc gcaggaagtg atgaacttaa taacgcaagc cggtggcaaa
    24661 gcattcgtgc tccaggcgga tatcagcgac gaaaaccagg tcgttgcgat gtttacagca
    24721 atcgatcagc acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc
    24781 cagtgcaccg ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg
    24841 acgggatatt ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc
    24901 agtggcggcg ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa
    24961 tatgttgatt acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg
    25021 gaagtcgccg cgcaggggat ccgggttaac tgcgtgcggc cagggtttat ttataccgaa
    25081 atgcacgcca gcggcggcga gcctggacgc gtcgatcgcg ttaagtcgaa catccccatg
    25141 cagcgtggtg gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa
    25201 gcctcttacg tcacgggaag ttttatcgat ttggcgggcg ggaaataaaa cagggaagtt
    25261 gtctgaccgg atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg
    25321 ttgctggcaa tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg
    25381 ccagtaccgt cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg
    25441 gtagtgaatg acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca
    25501 tcgtaagtca ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag
    25561 tcgtcgttga tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca
    25621 ataaacagcg gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact
    25681 ggatcaatct gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca
    25741 aaaccagaga tcgcatcgcc ggtgccgcct tcggccttca ctgcgttggt catgtaatag
    25801 ctgaaaccaa gataatcgca ggtgccttca cgcagtacat cgagatcgcc gtcttccatt
    25861 ttgatgttaa atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt
    25921 agctgcacat cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg
    25981 tccgggttac agcagtaggg atagagcggc accatcgcca gcatacagcc gactttcatc
    26041 tccgggttaa tgcgacgcgc agctttcacc gccagggcgc tggcgacaaa ctggtgatgc
    26101 agcacctgat acatcgtctc ttccgggttc tcatgctcgg tataaaccac gccggagcag
    26161 cagtaaccga acagcggtgc acgccagtta cgctggttgt taatttcgtt gaaggtcatc
    26221 cagtatttga ctttatgctt atagcgttca aacaccactt ccgcgaaacg gacaaagaaa
    26281 tcaaccactt tacggttggt ccagctaccg tattgctgca ccagatgcag cggcatttca
    26341 aagtgggaga gggtgatcac cggttcgatg ttgtatttca gcagttcatc gaacatatca
    26401 tcgtagaact tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc
    26461 gtccaggcga tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct
    26521 tccttgtagt gaccataaaa atcaacggct tcatggtttg gatagtattt gcctggcaag
    26581 acttctttgg tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg
    26641 ctcggccctt tgccgccttt gttccagccg ccttcgacct gatgagcggc aactgcgccg
    26701 ccccataaga aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc
    26761 aattcttaca ggaaatagta ccatgcagcc aaacgacatc actttttttc aacgtttcca
    26821 ggatgacatt ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt
    26881 caaaacgggt gatgtgcttc gtgtcgggcg ttttgaagat gacggttatt tttgcacgat
    26941 tgaagtcacc gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca
    27001 ggaaaatatg accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac
    27061 acagttttat gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa
    27121 tttcgcattt aatttcatga tttttaggag attagtctca aatcagaatt tattttagct
    27181 aacaggtgtt cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg
    27241 tttctccgga gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag
    27301 aggaaattgc caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg
    27361 tgttgctact ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca
    27421 gcctctatgg cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc
    27481 ctcatcaacg ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga
    27541 ttgccggaac ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt
    27601 tgatgattgt tatctggagc ctggcattgc tgggtattct gtttaaactg accatcgcgc
    27661 accgattcaa aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg
    27721 taatttatga aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg
    27781 gcgtggttta ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg
    27841 ccatctggca cgtcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt
    27901 atattgggca ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca
    27961 tcaggcatcg gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct
    28021 cgtccctgtt gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat
    28081 caggcgataa atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc
    28141 tcgatatgca gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg
    28201 ttattcatga cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc
    28261 gtaccggtac gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc
    28321 agtcggctgg cgctacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt
    28381 gctcgcgcca ccatctcttg tccggtatag cagccttttt taaagctgat gccgcccagc
    28441 gcctggaggt tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg
    28501 aaacccgctt caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg
    28561 cgcagtttat cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct
    28621 gccgggtgtt caaaccatag caaagtggtc gcgccttctt taactacctg tttttctttc
    28681 gaaggcagtt cactaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca
    28741 agcagcacac gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc
    28801 agttcagtca gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg
    28861 tcgcggaaca gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc
    28921 aggtgctgat cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt
    28981 tcgctgtccg cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc
    29041 ggtaaacggg cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag
    29101 tcctgagttg cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa
    29161 gtgctttcac tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagccatt
    29221 gaattgttgt gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa
    29281 ggcgaaaaac acgttacaat agcggggtac tggattttcg tgagacagga agaagatgga
    29341 cattaacaac aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat
    29401 ttcaatcatg ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat
    29461 ctttattcgt ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg
    29521 taaaccggcc gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga
    29581 acgtggtcct gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc
    29641 tgattcatgg gctggttgcc gctgttattt tactcatgcc ctggccactc agttacaccc
    29701 cgttatggat ggtgttactt tcgctggtgg tgtttgattg cgttcgcagc cagcggcgta
    29761 ttaatgctcg ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc
    29821 aggagtggag catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac
    29881 gttctgatgg cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg
    29941 aatggcggga tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc
    30001 aaagcatatg cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg
    30061 atttgctcgc accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc
    30121 agcgccagac ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca
    30181 tatccttccg ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc
    30241 agcataccga gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac
    30301 agcgcaacaa ttttaccttc gaggttcagg tcgtcgagct gatcccagac ggcttcccag
    30361 tcttcctgga tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc
    30421 tccattaatt tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata
    30481 atatcgcgga ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga
    30541 cccatattca taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga
    30601 atcgctttag tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc
    30661 aagtgaaaca ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa
    30721 atctggctga aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt
    30781 tgcatcaccg cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg
    30841 cagaacggct ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc
    30901 gccgattgtt ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc
    30961 tcgcttcacc gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggttgaac
    31021 gtttattaca ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg
    31081 aagtgttgta tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata
    31141 tcagcctgcg tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtac
    31201 cgttaggtga agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc
    31261 tgctgaatgg tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc
    31321 gacagacctt ctggcaccgc attaaacatt atgctgtgct ggcgggtatc gacagcgaaa
    31381 agctgtcacc gcatgtgttg cgtcacgctt ttgccaccca tttattaaat catggtgcgg
    31441 atttacgcgt ggtgcagatg ctactgggcc acagcgacct ctccaccacg caaatttata
    31501 cgcatgtcgc taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat
    31561 gtctgaaaag aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg
    31621 cgttttcagg ttttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg
    31681 gcatcaaaag cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta
    31741 acagcggcgt gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg
    31801 acgttagcgg cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg
    31861 cgcttgaaaa agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt
    31921 ttactgatat tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg
    31981 cgctggggat caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag
    32041 agaaagaaat gaaagctatc tggtgtgcaa aagataaaaa caaagcgttt gatgatgtga
    32101 tggcaggtaa aagcgccgca ccagccagct gcgacgtgga tattgccgac cattacgcac
    32161 ttggcgtcca gcttgacgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg
    32221 ttccgggtta ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga
    32281 ccagcggtaa ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga
    32341 aacggcagac ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg
    32401 cgtgcgcagt gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact
    32461 gagcggcgtc gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat
    32521 tattgtggtc ggcgattttg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc
    32581 gatgcgctcg cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg
    32641 ttacggctta agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt
    32701 cacggtggat aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat
    32761 tccggttatt gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat
    32821 cattaaccct aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt
    32881 ggcgttttat ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga
    32941 gcgtggcatc gcaattccta acctggcaga actgctggat ctggtagcgc tggggacagt
    33001 agcggacgtc gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg
    33061 catccgtgcc ggaaagtgcc gtccggggat taaagcgctg ctggaagtgg caaatcgcga
    33121 tgcgcaaaaa ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc
    33181 cgggcgactg gacgatatgt ccgtcggtgt ggcgctcttg ctgtgcgaca acatcggcga
    33241 agcgcgcgtg ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca
    33301 aggaatgcaa gttgaagccc tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct
    33361 acccggcggg ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc
    33421 ttcgcgcatc aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg
    33481 tacgctgaaa ggttcaggtc gctccattca ggggctgcat atgcgtgatg cactggagcg
    33541 attagacaca ctctaccctg gcatgatgct gaagtttggc ggtcatgcga tggcggcggg
    33601 tttgtcgctg gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac
    33661 tgagtggctg gacccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc
    33721 ggccgaaatg accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat
    33781 gttcccggag ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga
    33841 acgtcatttg aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc
    33901 ttttaatgtc gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta
    33961 caagctcgat atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat
    34021 ctggccaatt tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct
    34081 cttatcaccg cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc
    34141 ggtaaataat cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg
    34201 actacgacgc caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg
    34261 tctggaacga acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg
    34321 ttgtcgacac cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac
    34381 tggctgtaga agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgtcc
    34441 tggaagaaaa actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg
    34501 ccgactgcta cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga
    34561 gcatgcttga gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca
    34621 tcgaagagtc ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg
    34681 attacgctta cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc
    34741 cgtttgactc cggcggtcgt cgccacacat cgttcagctc tgcgtttgtt tacccggaag
    34801 ttgatgatga tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg
    34861 cgtccggcgc gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca
    34921 tcccgaccgg gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagacc
    34981 aggccatgaa gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg
    35041 agaaacaggc gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt
    35101 atgtccttga tgactcccgc attaaagatc tgcgtaccgg ggtagaaacc cgcaacacgc
    35161 aggccgtgct ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat
    35221 gaggaaccaa catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca
    35281 atgaactgaa aacgcgtcgt gagaagctgg cgaacctgcg cgagcagggg attgccttcc
    35341 cgaacgattt ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag
    35401 agaacgaaga actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc
    35461 gtcgtattat gggtaaagcg tctttcgtta ccctgcagga tgtggggggc cgcattcagc
    35521 tgtacgttgc ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg
    35581 acctcggcga catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt
    35641 ctatccactg taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat
    35701 tccacggctt gcaggatcag gaagcgcgct atcgtcagcg ttatttggat ctcatctcta
    35761 acgatgaatc ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt
    35821 tcatggtgaa ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atccctggcg
    35881 gtgccgctgc gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc
    35941 gtatcgcgcc ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg
    36001 aaatcaaccg taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca
    36061 tgatggaact ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc
    36121 tgttccgtac tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga
    36181 cgctggactt cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc
    36241 gcccggaaac cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat
    36301 ctatcggtat ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg
    36361 aagaagtggc agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag
    36421 tttctccgct ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct
    36481 tcatcggtgg tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg
    36541 cgcaacgctt cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct
    36601 acgatgaaga ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga
    36661 ttggcatcga ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc
    36721 tgttcccggc gatgcgtccg gtaaaataag cattacgtta tgctcacaac cccggcaaat
    36781 gtcggggttt tttaattgtg ctgcgcgaaa gtagacaaca attttcttgc ttcactattg
    36841 gctgcctgca tcaccatcca cggactgaac gcccacggtg tggcatcaat accgtgtaat
    36901 acatctgcta aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca
    36961 ctggtggtgc gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg
    37021 gtggcgcgat agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc
    37081 tcataacggc aacggcggat caccgcgtct tcgttgcttt ctcccagttg tgggtgccca
    37141 caaaccgagt tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact
    37201 aataattgtc ctttggcatt aaacagccaa ctggagaacg cgagatgtaa gcgggtgtct
    37261 gccgtgtgtg cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa
    37321 atgacgtgtt ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat
    37381 aattgaagag tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg
    37441 gatgccgaca ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa
    37501 tggccattgc aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac
    37561 tgaatatagc gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg
    37621 cgcatcagaa gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc
    37681 ttcaccaggt cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta
    37741 agggttgcga gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa
    37801 aagaaatcgt gagagagcgt cggagtcatg ccaacgtcga gactgatcgc cacaatatag
    37861 aggttgtaac ggttggtggt gtagttacag cgcgacaaaa ttcgaatccc tgtcgccagt
    37921 accatgccga acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc
    37981 agcaccgcca tttttggcac catgccaaac aggattaata taattcccga agaaatacac
    38041 acccagcggc tatgaacgcg cgtcacgcta accaggccaa cgttttgtga aaatgacgtg
    38101 tgggggaagc tgttaaacgt tccgcctatc attgtcccta cgccatcgac acgcagcccg
    38161 cgaataatat cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc
    38221 cccatcgact cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga
    38281 tcgaaaatcg gcataccaaa tgacatcggc gtgacaatcg caaaccatga agcatcatgt
    38341 agcccggata aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc
    38401 agtaatacgg cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa
    38461 attaagacag caaaggagat acctaaataa acgggattac catattgcgg attcccttta
    38521 cctccggcgg cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc
    38581 acaccggtaa ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat
    38641 gtggtgataa aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg
    38701 ttcatcccaa tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc
    38761 aggcggatcc ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag
    38821 caaaagagat ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca
    38881 ccaatcataa gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg
    38941 agtatgatca gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc
    39001 ccagaagatg agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc
    39061 cagagtaaaa agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc
    39121 cagtttttgc gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg
    39181 cttaaaccac gaaaatgaca agagaacctg acaatggaat tatctgtaat tttctcatat
    39241 tgatatttca ttaatcattc tggcatgtcg cactctcgca tttaatcgtt tttatctgga
    39301 tagcgctctt ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata
    39361 atttgaactc agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt
    39421 tattgcatcg cgaaatttga gaagacataa ctcattaaat gaaagggatg caaatgaata
    39481 agtttatcgc tgctgaagct gcggaatgta taggctgtca tgcttgtgaa attgcctgtg
    39541 cggtggcaca caatcaagaa aactggccgc tgagtcacag tgactttcga ccgcgtatcc
    39601 acgttgtagg gaaaggccag gctgcgaatc cggtggcctg ccatcactgc aacaatgccc
    39661 cttgtgttac ggcttgtccg gttaatgctc tgactttcca gtccgatagc gtacaactgg
    39721 acgagcaaaa atgtattggc tgtaaaagat gcgcaatcgc ttgccccttt ggcgtcgttg
    39781 agatggtcga tacgattgcg cagaaatgcg acctttgtaa ccagcgcagt tccggcacgc
    39841 aagcctgtat tgaagtctgc ccaacacagg cgttacgact gatggacgat aaagggttac
    39901 agtggataaa ggtggctcgc cagcgcaaaa cggcagcagg aaaagcgtca tcagacgctc
    39961 agccatctcg cagtgcagcg ttgctccccg ttaactcgcg taaaggcgca gataaaattt
    40021 cagcgagtga acggaaaacc cactttggcg aaatatattg cgggctggat ccacaacaag
    40081 cgacttatga gagtgaccgc tgtgtttatt gtgccgaaaa agctaactgc aactggcatt
    40141 gcccgctgca taacgctatt ccggattaca tccgtctggt acaggaagga aagattattg
    40201 aagcggcaga actttgccac cagaccagtt ccttaccaga aatttgcggc agggtatgtc
    40261 cacaggaccg tctttgtgaa ggcgcatgta ctttgaaaga tcactcgggc gcagtctcta
    40321 tcggtaatct ggaacgctac atcaccgata ccgcgctggc gatgggctgg cgtcccgatg
    40381 tcagcaaagt tgttccccgt agcgaaaaag tggcggtgat tggcgctggg ccagcagggt
    40441 tagggtgtgc tgatattctg gcgcgcgcgg gagttcaggt tgatgtcttt gatcgccatc
    40501 cagaaattgg cggtatgctg acttttggca ttcctccttt caaactcgat aaaacggtat
    40561 taagccagcg gcgagagata ttcaccgcaa tgggaatcga ctttcatctt aactgtgaaa
    40621 ttggccgcga tattaccttt agcgatttaa cttctgaata tgatgcagtt ttcatcggcg
    40681 tggggactta cgggatgatg cgagcagatc tgccgcatga agatgcgccc ggtgtcattc
    40741 aggctctacc gttcctgact gcccataccc gccagctcat gggattgccg gagtctgaag
    40801 agtatccgct gacggacgtg gaaggtaagc gagtcgtggt attgggcggt ggcgatacga
    40861 caatggattg tttgcggact tccatccgcc tcaatgccgc cagcgtgacc tgcgcgtatc
    40921 gtcgtgatga agtcagtatg ccgggctcgc gcaaagaggt ggttaatgcg cgcgaggaag
    40981 gtgtcgagtt tcagttcaat gttcaaccgc aatatatcgc ttgtgacgaa gatgggcgtt
    41041 taactgcggt gggcctgatt cgtaccgcca tgggtgagcc ggggccggat ggtcgccgtc
    41101 gtcctcgtcc ggtagcgggt tcagagtttg aattgcccgc cgatgttctg attatggcct
    41161 ttggtttcca ggcgcatgcc atgccgtggt tgcagggcag cggaattaaa ctcgataaat
    41221 ggggcctgat tcaaaccggt gacgtcgggt atttacctac ccagacgcat ctgaaaaaag
    41281 tctttgctgg tggtgatgca gttcatggcg cggatctggt tgtcactgca atggccgcag
    41341 gaaggcaggc ggcgcgcgat atgttaactc tgtttgatac gaaggcatcg tgatgaaatc
    41401 gttaatccgg ctgactgtat tggctgccgt acttgtgaag tagcctgtgt ggtcgctcac
    41461 ccttcagaac aggagttgaa tgccgatgtc tttttacccc ggctgaaggt acagcgactg
    41521 gacagcatca gtgcgccggt gatgtgccat cagtgtgaaa acgccccttg tgttggcgct
    41581 tgccccgtgg gggcgctgac gatgggcgag caggtggtgc agaccaattc tgcccgttgt
    41641 attggctgtc agagttgcgt cagtgcgtgc ccgtttggaa tgatcaccat tcagtcgttg
    41701 ccgggggata cccgacaaca aattgtgaaa tgcgatctct gtgaacagcg ggaagagggg
    41761 ccagcctgtg ttgaatcttg cccgacacag gcgctgcagt tgcttactga aagagaactc
    41821 aggcgagtcc gccagcagcg tattgttgcc agcggtgaga atcctctgtg aaagaaatct
    41881 ccgcgctgaa aggcgcggag aatctattag attgcccaac cacccgcgta aaatgcgacc
    41941 agtgcggcag taatgatcac cgtaccaata ttcaactttt gccattcgcg agcaaagaca
    42001 cggcctacga ccagtgtcac aaagcccagc ataataccgg taacgatatt acaagtcaga
    42061 acgatgaaca cggcacacac caggccagcc attgcgtcaa taaaatcgtt gaaatccagc
    42121 ttcgagacgt tacttaacat cagcaaaccc acgtacatca gagcgggtgc agtggcgtaa
    42181 ccagggatca aaaatgacag cggtgataag aacagaatca gcaggaataa cgcccccact
    42241 acggttgcgg tcaaccctgt tttacccccg gcggcggttc ctgccgctga ttcgatataa
    42301 accgctgcgg gcgctgcgcc caccagaccg gagaatattg aacttactga gtcactggtc
    42361 agggctttgc cgccattgat gatctggttg tctttatcca acaaattcgc ctgaccggcg
    42421 acggcacgga tggtgccagt ggcgtcgaac actgcggtca tcaccaatgc cagtacactc
    42481 ggaagtacgg ttggctggag tgcgcccata atatcgaggc tgaaaatcag agacttacca
    42541 tcttcgccag tcagacttgg catcgccacc agaccgtggt atttcaccgc tgggtcaaag
    42601 attaagccga taatcgaaat tgcgataatc accaacaaga tcccgccggg tacgcggcac
    42661 ttctccagac cgaagatgac cgccagcccc agcaagctca tcatcaccgg gaaggaggta
    42721 aacgcaccga gcgccactgg caagccttca atcgggtttt tgataaccat acccacaccg
    42781 ttagcagcaa tcagcagcag aaacaggccg ataccgatac ctgtaccgtg agcgataccc
    42841 atcggtaaat tacgtaagat ccaggtacgc acaccggtta cggaaatagc ggtgaagatg
    42901 acgcccatca gaaagaccgc gcccagtgcg acaggaacgc taatttgttg cccgagtacc
    42961 agactgaatg cggtaaatgc cgtcaaggaa atcgcacaac caatcgccat tggcaaattg
    43021 gcccacaacc ccatcagcaa cgagccgaag cccgcgacca gacaggtggc aacaaacaca
    43081 gctgcgggag gaaaacctgc tttgcccagc attcccggaa cgacgataac ggaataaacc
    43141 atggccagaa aggtcgttaa gccagccagt acttcctgac gaacggtact gccacgagcg
    43201 gtaattttaa aataattatc cagcgcgccc tgtggctttg gtgcgtccgg tgtttgtagg
    43261 atgtctccag acataatgat gtcctcacga ggatgttaaa gttttattat tagttgcgtt
    43321 cgtacaccag acgaccatca acgtaggtgc ggtagatcga acggtcatcg cccaacgtca
    43381 tcatcacgaa caatttgtcg actaaagaaa cagagttgtc atagcgcagc tgctgtagcg
    43441 gagtggcggt gggttccatc accacgaaat cagcctcttt gccaggtaaa aagttgccaa
    43501 tcaaatcgtc aaggcccaga gatttcgctc cgccgagcgt ggccaggtaa aacgcttcat
    43561 atgccgagag gcgatagcct tgtaattgca atactttgta ggcttcgttc agcgtttgca
    43621 gcatgttgaa agtggttccg gcaccgatat ccgttcccat gcccacttta actttcttct
    43681 gccatgcttt tttcaagttg aataagccgc tgccgaggta aaggttggag gtcggacaga
    43741 aagcaatgct ggatttggtt tcgctgagac gatcccactc tttttcttcg agatggacgc
    43801 agtgagcaaa gacacagttt ttaccggtca ggccgtactg atggtaaaca tccagataac
    43861 catcatggtc aggataaagc gatttcaccc aggcaatttc atctttgttt tcacagagat
    43921 gggtatgtac ccacgtatcc ggatattctt ctttcaggcg ttgcgccatc gccatctgtt
    43981 caggagatga ggtcggggcg aagcgtggcg taatcgcata tagcagacga ccatttttgt
    44041 gccagcgttc aatcagttct ttgctttggt gatagctgct ttcggcagtg tcgagcagat
    44101 aatccggtgc gttgcggtcc atcatcacct taccggcaat catacgcata ttgatatgac
    44161 tggcggcttc aaacagcgca tcaacagatt gcggatgaac agtgccaaac accagcgccg
    44221 tggtggttcc gttacgtaaa agctgcttga tgaagaatgc cgacatttcg cgggcgtact
    44281 ctaaatcctc ataacgacgt tcagtaggga aggtgtgttt attcaaccac tccagcaatt
    44341 gttcaccata ggcccccacc atttcacttt gcggataatg gatatgtgta tcgacaaagc
    44401 ccggtactat cagtttgccg cgatagtcgc gcacgcgaat agtgtcagga atttgatgct
    44461 ttccgtcttc ccattcgcca aaccattcca cttttccctg tttaatgagt aataaaccat
    44521 cctcaataaa ccgcagcgca gaggcaatct cttccgggtt atcgaccgta cgggtgacat
    44581 caataaaact gcctcgtacc gctttcaacg tgtgttctcc tgacatcatt gactccttta
    44641 ctgttaatcc atctcttccg ctgaggtaat accaggcaga acgttttcct gtcggtagcc
    44701 accagggagg ataatattta ataaaatcgc agttaacccg ccagcacaaa tagggttttc
    44761 aactagtaca taaatagagg ctggcaatat tttaaaaatt tcgggatcgt aggagacgcc
    44821 aagtcctaaa cctaaagaag tggcgacaat aagtgtttct cggcgcttta aaccgttggt
    44881 gatgattatg cgaatcccag cgatggcaat catggaaaac atcaacgtca ttgcgcctcc
    44941 cagaactgcc gagggaatgg tcgtgaagaa gccgccaatc atcggaaaca aaccgaggat
    45001 aaccagcatt accgcgatgg ttcgcccgac ataacgtgaa gcgacgccag tcatctgaat
    45061 aaccccatta ttttgcgcaa acgtggttaa gggtaatgac ccgacagcgg aggcgataac
    45121 agaaaccaga ccgtctgcca gcacgccgcc tttcagtcgg gactgatact cttctccctg
    45181 aatgggtcgg cgggaaacca ttgccgtggc ggtgatatcg ccgacagctt ccagcacgct
    45241 aagcagataa atcgtgccaa ccaccaggaa ctgatggaag ctaaaactaa agccgtattt
    45301 gaacggatgc gggatggtga ttaacggcaa attgcgcata ctgctgaaat ccaccatgcc
    45361 caggcataac gatgcgatat agccgacaca tagcccaatg gcgatccctc ccatgcgtag
    45421 caacggactg cgacagcagt taaagccgat caccacaatt aaaaccaata aaccaacgcc
    45481 gagatgttcg taattgccga acgtaccgct gcttttggct gcaaatcctc caccaaaatc
    45541 gataatgccg actttaatca ggcttaagcc gatcatcagt accacaatac cgctgacggt
    45601 aggcgtaata acccgacgta aatagggcaa gataaatgaa gatccgacaa ccagaaatgc
    45661 gccaacgaag gagacgccga gaagcgacga catgattaac tcttcgtgaa aaccgtcgct
    45721 tttcatgctg ctgcctagcg caatcatgac cgtaacaaat gaaaaattga ctgactgaat
    45781 tgagagtagg ccagaaccga cgatgccgta gcggtttact tgtaaccagg taccaatacc
    45841 agaggcgatc atcgccatag aaacaagata ggcagttgtt tcagcggaaa gctgtaaggc
    45901 cgcccccacg attaacgcgg gggttaccat cggaacgaaa attgccaaca gatgggtaat
    45961 ggcaccaacg agagcctgat gaaagggagg gcgatcctcc agttcaaata taaggtcaga
    46021 acctgcattg tttatatcag acatcccttc actcctttta aagtaaattt gatcatctga
    46081 attagtaaaa taaattccca cgaatttggg gcgcaggacg ccttgagaac ggcttatctc
    46141 attttcgcag tcactattta gtcatcgtcc aaacagagaa acaggccgcc gaagcggcca
    46201 tttattattt atattttttc cagcgcagtg agtattttct ccggtgtgaa atgccattcg
    46261 cgtaaccaga tgccgcatgc atcgtgaatt gcggtagcaa tcgccggagc tgcgccattt
    46321 acaccgattt ccgagatcga tttcgccccg aacgggccga ctttatcgtc gctcggcacc
    46381 agcacagcgc ggaaatcacg cggaatgtca ccaattttcg gtgcgccgta actgcgtaaa
    46441 tcacgcgtta acgggtgacc ttcggcgtca taaatgatct cttcgctcat actgtggccg
    46501 atagctcgca gggtagcacc gtagatttgt cccaatgcta attccgggtt gaccggtgta
    46561 ccgcagtcca gcaaggcgta gaatttatcc aggcggattt cacccgtacg cgtgttgacg
    46621 gcaacttcag cgaagtttgc gccatacggg aaggcgaaat caggcgtgat ataactgcca
    46681 gttcccacca gtgaaccaaa gccggtgccg gtttcgcctt tatgagcaat atccccgaag
    46741 ctgacttcgc ctttcttgcc gcgcacgacg cccggcgttg ccagttgaac atctgccact
    46801 gcctcaccca acatttgcgc gccgtggaac agaattttct cccgcagatt ttccgctgcc
    46861 aaacgcgccg cgttacctga gaagcaagta ccggacgagg catatgcgcc tttatcaaac
    46921 aacgcatgat cggtatcacc ggagataaca tgcacgtcct gcggtgggca gtgcagcact
    46981 tctgctgcca gtttcgtcac tacagtatcc agaccagtac caatatccgc accgccagaa
    47041 tgaacgataa aggtgccgtc cgattccagt ttgatcatgc agttagcctg atcgatatcc
    47101 gggatacccg atttctgcat gataatggcg acgccgcgac cgatgtgcca gtcaccattt
    47161 tgcggtttcg gtgacgacca ttggatcatc tcgcgaccct gacgcaggat ctcttccagt
    47221 gcacagctgg cggcggaagg aacggaggtc ggcgctttac cttcaccgat tgcaccgaga
    47281 attttcagct cttgcccttc gtgtacccgg ttacgttcga taatttccag ttggtcgatc
    47341 tgtaactgtt cagccagttc cgctaatgcc atggtgatag cgaagttacc tttcggtgcg
    47401 ccataaccct gataagcacc atttgggcaa atgttgctgt agtaggtggt gacctggaaa
    47461 tcgacgttat cgcacggata taacggcagc gacagcgccg gtccgttaca cggtacggtg
    47521 agtgagtggt tgccgtaagg gccagtgttg gcgcggaaat ccatcttcac tgccgtcagg
    47581 cgaccatctt ttttcgctcc cagtttgacg gtgactttcg cgacgtgacg agaggtgtta
    47641 gcaataaact cttcttcacg ggtgtagcgg aacagtaccg gacgcccggt cacgcaggtt
    47701 gcccaggcgc acacttcttc cagcaggatg tcctgtttgg aaccaaaacc gccgccaact
    47761 cgctctttaa tgacatgaac tttatgctgt ttcatgccca cgaggcgcgc gacctggcgg
    47821 cgtaagtgcc atggtacctg ggtggaggcg tggataacca gacgatcgcc gtccatccgg
    47881 gtaaagcaga tatgtgtttc agtcgggcac tgctgtgcct gcgttgagtt ataggttcgc
    47941 tcaatgatca catcggcatc ggcaaagcct ttgtccatat cgccaatatg accatgaata
    48001 ctggcggcga tatttttgcg agggcgagaa ccgatcggga agttgatgat catatgctcg
    48061 ccgcgctggg ctgcatggct gttatcgtct tccagagtat ctggcgcacc agcaacatac
    48121 accaccggtt catcgtgcac gacaggcgca tcttccgcca ttgcttcgtc gatcgacatt
    48181 accggcttaa gcacttcata ttcaacgtcg atgagcttca atgcttcgag cgcaatttct
    48241 tcactttctg cgacgaccgc agcaacacga tcgccgacgt gacgcatttt cttgccgaac
    48301 atacggcggt caagcggtga cggttccggt gcgctctgac cacccggggt atagtagata
    48361 tccgggcaat tcaggtgagt aataacgtga acgacgcccg gtaaggcttc agctttgctg
    48421 acatccagat gagtaatcag tgcgtgagcg tgtgggctac gtaacatttt aatgacgcag
    48481 gcgtcagccg ttacgcggtc ttcaacatag cagggtttcg cctgcaccat tttcgcggca
    48541 tcagttttag gataatgctt accaatgact tctaggtcgt cacggaaagt cggagcgata
    48601 tcaatggtgg cctgcggatt atttttacgt gcaaccgcca gttcaatgac ctgatagtat
    48661 tgctgccagc cagcatcgcg gctgaacaaa ccagaaagcg cgtcgtcgat ctcttcgcgg
    48721 gtaggtgcgg cgatgcgatc gagaagatcg gtgataatca gagctgcagc tggatcgtta
    48781 taaccagact gcaccacgcc aacatcaacc attgcctgtt gaaccagact taactcgttc
    48841 catttgccca gagattctgc ggtacgaata tctgccttct ctaactgtgc ggcaataagc
    48901 aaggacgcgt taacgatatt accgttaaag attattgcgt cagaaccggc aaacccgaaa
    48961 ccatcatcac tgttgcgtac agagtgcatt cccatgttaa acaacatctt ttgcacgttt
    49021 tcgcctggat taacggttag ctcctgaggc gcgccattta aagtaaagtg gatgatcata
    49081 cggcttcctc cccagcctgt tggcagtcgg catacaggtc ggctaccaga actcccgtga
    49141 tatagcgttt ataggccacg ctgccccgca aatcttcctg cgggaaaatg gcgttggcga
    49201 cagcctgttc aagtgcattg ccttccagat tttgtttttc gacatcatgc agacgcagtg
    49261 ctttactggc gacgccatcc agcgcaattc gcataccgtc gtggtctgtc attgcaacgg
    49321 ctgccgtcac gacggttaaa cctgcctgag aacggctaat tttgcgggtc gcacaggtgc
    49381 gatacggatc tttaatgata atttcggtta gcaggcgatc gcatgggcag gccaggtagt
    49441 cctcgattga cagcgtttcg ccgttgccaa aaaccagttc agcatccagt gccaacagga
    49501 cgggaagcag caccgactct tcctggcggg cggcgatttc accaccaatg gtcgactgat
    49561 tacgaacatg gcgtgagtaa acaaaaccga gggcttcacg cagcgctgca ggaataaatc
    49621 gcgcatcacg cagtggctgc aagcgagata ttgcgccaat ccgcagtgca ccgttatccc
    49681 agtcaaccca gtccagttcc agatcctgta gggaaatggc aatcttttta tcggtacggg
    49741 ttggtgtagc attgagtttg ctgcccccgg cgaaccagac ggcttcatcc tggtagcggc
    49801 gcttcagttc cagcgcctgt tcgacagagt cgggcctgaa aaattgttca atcatcttct
    49861 ttccttattt aaccctcttc cctgcgggaa gagggcatct gtcatttatg ccagcgcatc
    49921 catccgacgc cacatactgg cagcggcttt tctggcttgt gcataaatgg aatcgcaatc
    49981 gaagttaaac tgacggtctt catagaccat cacaccattg accattacgc tgtgaacgct
    50041 gcctgagccc ataccgaaag cgatatgccc ggcgatattg tctgccagca gcggcgtcgg
    50101 cgagttgtaa tcgcaaatgg ttaaatcagc tttgtaaccg gcttccagaa gcccaaattt
    50161 cgcgccaaag ttgcggctca tcagttcgtt accgttagtc agggctttgg caaaactgtc
    50221 aggccacagc ggaccacccg catcgcgatg tttaaagaag gcaaatttca tctcttcaaa
    50281 catgtccgaa ccaatgccgt ccgttcccaa cgccagattg cggatgtcgc taaggtgatg
    50341 gttgtagccg acatggttgt tcatgtttga acgggcgtta tgcaccagga acgcatcgcg
    50401 ctgattgagt agggcgatgt catctttcga caagtacagc ccatgagcga ccagcgtttt
    50461 gctgtcgatg agatcgaatt gcgccagtcg tgccagcagg tctttgccgt accagtggtg
    50521 actgtaggaa acgtcgtaaa ggtcttccgc agcgtgaata tgcaaaccac ggcctgtggc
    50581 tttcacggct tcatgcagca tctccagacc ggcatccggc acggtaaacg gcgcgtgagc
    50641 accgatatgt gcttccacca gatacggctc gcttgtcgct ttcttcgcct catcaatcaa
    50701 acgggcgaaa cggatgtttt cttctacacc ttcctgcaac tctttgatgc cgttgttacg
    50761 gtcagtagtt tcaaaacagg tcattgcgcg caggccaact tttaaaaatg cgtcgcgcaa
    50821 tgtggagagc gacccgccga tatacgccgg agaggcatgg tgatcgataa ccgatgtaca
    50881 tccgctctta atcgcttcca gggaacaaat cagtccgctg taatagagcg actcttcatc
    50941 aagggcgcga tcgagccgcc accagagatt tttcagcgtt gagatgaaat ccgggcaggg
    51001 ggcgatgttt gccataattc cgcgggaaag ccccgagtaa aaatggttgt gcgagcagac
    51061 aattcccggc atcacaattc gaccatgcat ctctttgtag ctggcatcgg ggtagcgttg
    51121 cgtcagggca tcgccgatag cgacaatcac gtcattttcg atagcgatat caacgccttc
    51181 ctgcactttc gccgggtgta actgcactgc ggtgacattc ttcagaatca acatgattac
    51241 acctccacgc ggcccagcag ataatgatga tgctggtgga catggctgat gatgcggcac
    51301 atatcgttca gctccggtgg tacgttgtta aactgacctt cgctgtcgat gtttaacacc
    51361 cagctttggt tattcagacg tactcgtacc cggcaatctt ccacaaggaa gcctgggttg
    51421 ctgctgttat caaagtcttg cgccaggctg aagacggtga ttttgtcttt gtatggttta
    51481 ccgttccacg gacagaactg agcgcagttg ccgcattcgt tacagtaagc gtcgaggtgc
    51541 agcgtctgga atctattctg gaagccaggg actgcaatgg agacgttggc gcggttcggg
    51601 cagacatcca cgcacttgct gcaaacgtag ttacattcaa ggcagcgagc agcttcctgc
    51661 gcgacaaacg catcacgatc gtcactgttc accagcgtga tagagatatc gcctttacgt
    51721 tgatagattt ccgccggatt gacgttgttc cagtatttat tgttctggtg ggaacggata
    51781 ttttcccgac taaggatggc atcggtcgcc cgacgcgcgg ttccgacagc agcgacaatg
    51841 gaggatggtc cgcgctgtac gtcgccgatc ataaagacgt cagtcagacg agtttcgcca
    51901 ttatggtcga cgtctggcca gccgtttttg tccagcggta cgcccatcgc attcagggct
    51961 tcagtatcct gctgttcacc gatggcggtg atcagactgt ctacatgcag tgttactgtt
    52021 tcgttggttt caaccggacg gcgacgacct ttctcatcgg gttcgccaag cgacataacg
    52081 cgcaaggtta aggtgccatc agcatcgaaa cattccggat tattcaggaa acggaactcc
    52141 acgccgtcgt gcaacgcttc ttcatactct tcgcgccatg cgggcatctc ttgcagtgaa
    52201 cgacggtaaa cgatcgttgc tttttctacg cctggaacgc gtaacgccgc acgagcgcag
    52261 tccattgcgg tgttacccgc cccgacaacg accacatgtt tgcccagctt gagcgctgta
    52321 cccttgttgt attcacgcag gaaggggagt gatttccaga cattttggtt gtcacccgcc
    52381 agtttcacac cgctattttt atcagtgccg gtggcaatca gaacatagtg gaagccctga
    52441 ttttttaact gctcaacggt taaatcgggt gagcagccat actcaaattt cacgccgtga
    52501 gcggcaacaa aatcgatatc gtgctgaatt aactctgcag gaatacggaa ctgaggaatg
    52561 atatttttca ccacgccgcc agcattggct tcgcgttcaa acagcgtaac cggatggccc
    52621 gctctggcaa ggaagtaacc tgctgccaga cccgccggac ctgcaccaat cacggcaact
    52681 ggatggcgtg aaccagaacc ggctggtttg tgccagcgtt gcttatattc atcccaacct
    52741 ttttccagcg cgactttttt cagttcgcgg atattcagcg cactgtcgta atccaggcgg
    52801 gtacagttgt attggcactg gtgatcgcaa atatgaccgg taatggcggg cagagcgttg
    52861 cgttggtaga tgagttccag cgcgtcggca tagcggtgtt cgccaagcag acggatgtat
    52921 tccggaatat cttgcttgat agcacaggca gtaacacagg gggcaacgta gcagtcggtc
    52981 agcggcaggt cttctgccac ttcaatacgc tcttctggct tccagtgttt ctgggtgtat
    53041 tccatggtta acgcatctgc tgccagtctg ttcagtcgtt cgacgtcaac atggtcaagc
    53101 ccccaggcgt cggagccttc cagctcgcgc atgcaggcac ttaagcgcag atagccgcct
    53161 ggtttcagca ggtcggttgc catagtaata gggcgaatac ctgtatcaaa aatatcgcgg
    53221 atagtcagct gactggcacc accggaataa gaaatgggca gtttgccgtc aaaggcgcga
    53281 gagagaactg ctgcaacatt gatggagagc gggaacagcg cacggcctga catatacatc
    53341 tcttcaccag gcagtgcgcc tttattgttg atggtgccga gagtgttagt cagttttacg
    53401 ccaaagccga gtgatttttc ttttgccagt gccatcaggc gttccagcat ttccagtgct
    53461 tgcgtcagct tgaggtcgtg atcaaatgac tcttctttta agcctatgta accgaaaccg
    53521 cagacatcga ggatctcacg aacacgcgcg tatcccagta aggtcgggtt aagtttcaca
    53581 aaggtgttga gccctttttc ttccagcatg tagcggcaaa tggcttcgat ttcatgcgga
    53641 ggacagccgt gcatggtgga gagggtgacg ccatgcacca tactggtggg gatgcgagcg
    53701 ggtaaggctt gcaagctttc gcgtttttcc tgcaatccgt ggcgagctaa aaatgcgtca
    53761 tcctggagta atttattcag cgtatcgcga tattgagcga atttcggatg gtcagatgcg
    53821 tccatcatat tgtcgatgaa ctgttgcatc ggcggttgct taataccttc gaggttgtaa
    53881 ccgacgctca tattaaagat gaacgattta ccggaatcag aaggctggaa catcgcttcg
    53941 agaaggtgca gggcaaacca ggctttgagg tattcatccc aggctttaag cagggtaaat
    54001 tcggtagacc attcggtgtt aaagcactcg tcttcggcat cgatacaggg cttttccagc
    54061 tccaggcggt caagaatttg gacggttttt agttcgatga agcgtccgcc agtcagccag
    54121 gaagtgacaa tattttgcgc gagctgcgtg tgcgggccag cggcagggcc gacgggagtg
    54181 gcacaggttt caccgaagac gctaacagtt ttacctttta cgggtgagta aaactgttgc
    54241 tcgggaatac caaagattga gcgttgttgt tggtattcat caaatatgcg cgtcaaaagt
    54301 tcctcaaacg gaatgggacg cataatatcc cccataaccc tctccttagc ttatacagat
    54361 atcattgagt gaccggtgtt gagaaatccg gtggtttagg gggatagagc aacaatcgca
    54421 ccactcttgc caggtttggc gggaaatata aaaaaatgtg aggcggctcg aaacttaagg
    54481 gctttttgtg aatgaactca caggtgctaa atgagaatta aaatcactcc tgaaaattat
    54541 tattgccagc ccatttatca atttttctct tttctcaaaa tcagtgattc cttatcatta
    54601 tgataatgag tgccggaagg attaaatatt tgaacgcaat cgtttaacta cgttttctaa
    54661 atttcagtat acctttcctt cgctgtaata aagtcgtccg gagtatcaat atctaaaatt
    54721 atttctgcat tttctatttc gacggaataa tgatctccca taaggagtgc ctgacgcata
    54781 ttggtgacat tgggtcgttg gattgcctgt atcaagcatg gttttgacac taaaatcgga
    54841 tggccgggga tgccattatg gagtggcagt attgcgccat catttcgtaa cgaccagatt
    54901 tttctaaaaa tatcgatggt gagggttggc atgtcaccgt gggtgagaaa acaatgttct
    54961 gtttgcactg cgggtactgc ggccttgacg gatgttagta aaccctgcgc ataatctggg
    55021 ttgtgaataa tagtgatatt gctctggttc gcataacgtt cgtgcagttc attacaacga
    55081 tagccggtga ctaaaataat tcggctacaa aactgcaacg catttttgat acttgtatca
    55141 agaattgttc cctgttccca gggtaacatc attttccatt gccccattct tgatgataat
    55201 ccggcagcgg taattataca gtcgatggct gacatagtgg ttccattatt tataggggct
    55261 aataacgcct gataattgcg ttacttcctg atggattaag ttggggataa tttatcccag
    55321 agaggtcata aagactcgtg aaaagtatag ttgacccatc ggcattagtc attgatttag
    55381 gtgcgcagaa acgtcccact gtgatttctg ttgttggtgc tggggggaaa accagcttgc
    55441 ttttttggct ggcagaactg ctccaggcta gcggcaggcg tgtgttaatc acgacgacga
    55501 cacatatgtt tatgccaacg tctcactggc ccgtggtttt ctgtcgtgat cccgccatgc
    55561 ttcctcatgc gtctcttaca tcccccattt cattttgttt tcacagctgg aaagcgaacc
    55621 agggaaaagt gcagggattt acgccagaag cgattgatgc actggtgcaa cgaccagagt
    55681 gtgacgtaat tctcattgaa gcagatggct cgcgtggaat gccgttaaaa gcgcctgatg
    55741 agcacgaacc ttgcataccc aaaagcagtt gctgcgtgat tgctgtgatg ggaggacata
    55801 ctttgggcgc gaaagtgagc acagaaaatg tccatcgctg gtcgcagttt gctgatatta
    55861 ccgggttaac acctgatgca accttgcaac tgagcgatct cgttgcgctg gttcgccacc
    55921 ctcagggggc gtttaaaaac gtaccgcaag gttgtcggcg agtctggttc attaaccgtt
    55981 tttctcaatg tgagaatgcg attgcacaaa gcgagctcct tcaaccgctg caacaacacg
    56041 acgtagaggc aatctggctg ggcgatatac aagaacatcc tgcaatcgcg cgcagatttg
    56101 tgaattagcg gcccgggtat tccgggttac tgaaaggata tccattgagg ttggtatgaa
    56161 tattttcaca gaggctgcaa aactcgaaga gcaaaattgt ccgtttgcga tggcgcaaat
    56221 tgttgatagc cgaggctcga ctccccgcca ttctgcacaa atgttagtgt gcgccgatgg
    56281 ttctatcgtc ggtacaattg gtggcggaat ggttgagcgg aaggtgattg aagagtcact
    56341 tcaggcattg caggaacgta agccgcgatt attccatgga cgtatggctc gtaacggtgc
    56401 ggatgctgtc gggtcagatt gtggaggtgc aatgtcagta tttatcagcg tccatggtat
    56461 gcgtccacgt ctggtgttga ttggcgcggg gcatgtcaac cgggcgatag cccagagtgc
    56521 cgcgctatta ggatttgata ttgccgttgc cgatatttat cgcgaaagcc tcaatcctga
    56581 actattccca ccatcaacca cgcttctcca tgctgagtcg tttggtgcgg cagtggaagc
    56641 actggatatt cgccctgata attttgtcct gattgccacg aataatcagg atcgtgaagc
    56701 cctcgacaaa ctcattgaac agcccattgc atggttgggg ttgctggcaa gtcgccgcaa
    56761 ggttcagctt ttcctgcgtc aattgcgtga gaaaggcgtg gctgaagaac atattgcccg
    56821 tttacatgcg cccgttggtt acaacatagg tgcggaaacg ccgcaggaga tcgccatcag
    56881 cgtgctggca gaaatattac aggtgaaaaa taacgcgccg ggtgggctga tgatgaaacc
    56941 ttctcatcct tccggacacc agctggtggt gattcgcggt gcgggagata tcgccagtgg
    57001 tgtggcgcta cgtctgtatc atgcgggttt taaagtgatc atgctggaag tggaaaaacc
    57061 gacagtgatt cgttgtaccg tggcgtttgc ccaggccgtg ttcgatggcg aaatgacggt
    57121 cgaaggcgtc actgctcgcc tggcaaccag ctctgcggaa gcgatgaaac ttaccgaacg
    57181 cggattcatc cctgtgatgg tagatcccgc ctgttcattg cttgatgaac tgaaaccgct
    57241 ttgcgtggtg gacgctattc tggcgaaaca gaatttggga acgcgggcag atatggcacc
    57301 agtaacaatc gcgcttgggc cgggctttac tgcagggaag gattgtcatg cggtaattga
    57361 aacaaatcgc gggcactggc tcggtcaggt gatttactct ggttgtgcgc aggagaatac
    57421 cggtgttcct ggcaatatta tggggcatac cacccgacgg gtgatccgtg ctcctgctgc
    57481 aggcattatg cgatccaacg tgaaattagg cgatctggtg aaagagggcg atgtgattgc
    57541 ctggattggt gagcatgaaa ttaaagcacc gttgacgggg atggtgcgtg gcttgttgaa
    57601 cgacggcctg gcagtggtcg gtggttttaa aattggtgat atcgatcctc gtggtgaaac
    57661 ggctgatttc accagcgttt ctgataaagc ccgggcgatt ggcggcggcg tacttgaggc
    57721 gttaatgatg ttgatgcatc agggcgtgaa agcgacaaaa gaagtgctgg aagtggctta
    57781 aatgaaaatg gccggatggt attacccacc cggccttaaa attactgaca aatcaccgtt
    57841 ccggttttgc cttcaatccc ctctttcgct ttgctcaata cggtaatcag tgcttcacga
    57901 cctgcgcgag agcgagcaaa cgaggcggca gcttccactt taggcagcat agaacctttc
    57961 gcaaaatgac cttcttcgat aaagcgttca gcatcactta atgacaggcg atcgagccac
    58021 tgttcattct ctttaccgaa gttaatggcc actttttcta ccgcagtcag aatgatcagc
    58081 atatcggcat caatcatttc tgctaaacgg gcgctggccc agtctttatc gataaccgcg
    58141 ctggcaccac gcagatggtt accttcacga ataacaggaa taccaccacc gccaacggtg
    58201 atcaccacct ggcctgcatc taccagagct ttaaccgttt ctttttcaat aatatcaacc
    58261 ggttttggcg aggcaacaac acggcgatag ccgcgaccag catcttcttt cagagtgtaa
    58321 ccctgttttg tcagttgctc tgcttcttgc tcggtaaaga acgaaccgat cggcttggtc
    58381 gggttgagga atgccggatc gttagcgtcg acttcgacct gagtaaccag cgttgctacc
    58441 ggtttattaa tgccccgaga aagcagttct tctcttaagg cgttttgtag atcgtaacca
    58501 atataaccct ggcttaaggc aacgcagaca gacatcggca gcattggcga gtgcgcttca
    58561 gtttttgcag cagcttcaaa ggcctggtta atcatgccca cctgcggccc gttaccatga
    58621 gtgacgataa cttcatgtcc ttgggcaatt aaatcaacaa ttgcctgaga agtaattttt
    58681 accgctttca tttgtccggc caggtcatcg cccagcgcat tcccgcccag ggcgagaaca
    58741 attttcttac tcatttttcc tcactcatca ttcttgattg tgtatagatc cctgacagtg
    58801 gttagagcac gggagggaca aacggtttgc gtcgcaggaa acgacctcgc ccggctttgc
    58861 ctgtaaaggt gccgtcacag aaaatcgttt caccacggga taaggttctg acaatcgcgc
    58921 cctgacaggt aaaaccctcc catggcgagt agtcggcgtt gtcgtggaga tggcgatgct
    58981 gaatttgttg gctctgacgt gggtcgataa tcaccacgtc gccatcggaa ccgggcgcta
    59041 ataatccttt ttgcggccac aggccaaaca gcctggcggg cattgcgctg gttaattcaa
    59101 caaagcgttc cggtgttata cgtcccgtca tcacgccact ggaaaacagt aactgcatgc
    59161 ggttttccac accgggtaag ccatttgggc agcgactgaa atcgccttta gaaatttgca
    59221 ggcgttgagc catcgagaag gtgcagtgat cggtcgccac cacgtcaatc gcaccatcgc
    59281 tgatgccaca ccacagtttg tcctgctcgc gtacgttacg cagcggtggg ctaagaatga
    59341 acttcatgcc gtcttctgta tcgtaactgc gttcgtccaa caggagatat tgtgggcagg
    59401 tttcaaccca gactggctgg tgattcgcac gggcaagacg cagataatcc agacctaagc
    59461 cgttagacag gtgcacgata tagagcgggg cgttaccggc aatttgtgcc aggttaatca
    59521 tgcgggcgat ggcttccgct tcgcattcca gagggcgact caaggcatga tagcgcggcg
    59581 cggttaaccc tgcggcgata aattccgccc gcttgctggc gatagccgca tcattttccg
    59641 ggtgcacggt ggtcagcgcg ccggattcat gcagacggcg taatgcctgc aaaacctcgt
    59701 catcgttgag tttgtattga taggttaaat agagtttaaa actgctcagt ccttcctcga
    59761 ccatcatcgg gatttcgtcg aggattgcgt gattaatgtg ctggatcaca ccgtgaaagc
    59821 tgtaatcgat gaccgcttta tgggcggcat aaccacgata aacctccagt tgatggcgta
    59881 accgacagcc gtttgggcca aatcccatat ggtcaataat ggttgttgta ccgccacacg
    59941 cagctgcgcg ggtaccggta aaaaaatcat cacaactgcg cgcgatgccg acatcaatat
    60001 tgaaatgcgt atggacatcc acgccgccag ggaaaacgta acagccagtg gcatcaattt
    60061 cttcatacgg gagctgcggc gaaatattgt tgcccaactg gcgaacaatc ccgctttcaa
    60121 tcagcaaatc ctgtttggct tgtccatctg cgttaacgac agtgccgttt ttgatcaata
    60181 cgcgcatagc aaactccaaa aaccctccgg cgaaccggag gggaataagt gaagagtaat
    60241 tactctgttg ccagccagct taacgggatt gcagcgtaca tcgcggcaca ggtcaccagg
    60301 tgagatttcc aggttttttc gttaggtgca tgcgcttcag gttctttacc cgggccaaag
    60361 ccgatcaccg gaatgccgtg acggcccatg atagatacgc cgttagttga gaaggtccac
    60421 ttatcaacaa ccggcgcttt gccaaacagc ccttcgtagg cattcaccag tgctttaacg
    60481 gtgaagtgat cttcttccac tttccaggtc gggaagtagc attcggttgg gtaaaccagg
    60541 ccagtccagg acggacggtc gtagttgtac atagaaacaa ccgcgttagc tttctgtact
    60601 gcaggcaggg cgcggatttc gtccagcgcg ccttcccagg tttcgcccca ggtcagacgg
    60661 cggtcaatag agacggcgca gctatctgct acagcgcaac ggcttgggga tgtgaagaag
    60721 atttcagaaa cggtgagggt gcctttgccg aggaattcgt cataacccag acgttgggag
    60781 agttcttgta attcgccaag aatcggaccc attttgaaaa tggcgttgtc accgcgttct
    60841 ggcgcagaac cgtggcagct aacaccctga acatcaatac gaatttccat acgaccgcgc
    60901 tgaccacggt atacctggca gtcggttggt tcggtactga ccacaaattc cgggcgaatg
    60961 ccggattgtt caataatgta ctgccagcac agaccgtcgc agtcttcttc ctgcacagta
    61021 ccggtaacca gcagggtata ttcatcttcc agaccgaggt ctttaatgat tttaccggca
    61081 taaaccatag atgccatgcc gccttcctgg tcggaagtac cgcgaccacc gatcagctca
    61141 tcagtttcca tgccttcgta cggatcgaag tcccagtttt tgatgttgcc aatgccgacg
    61201 gtatcgatat gagcgtccat tgccaccaga cgcgggccgt ggccgatata accgagaacg
    61261 ttgcccatcg ggtcgatttc aactttatcg aagccgactt tttccatctc ttctttaata
    61321 cgatgtacta cgcgtttctc gtcgcagctt tcactgggaa tagcaaccat gtcgcgcagg
    61381 aagcgagtca tatccgcctg gtaatctttt gctttttcaa gaatcagttt gaatggaata
    61441 ttcttagcca ttatttttct ccagattttt tactccccgt tttgcaggga gcagttccaa
    61501 tagggtgatt aaggtgctac agcgtgtttg ccttcccaga caacttcgcg gtagtgcttc
    61561 acgtcggtgt cgccttcagt gctgataacc agcaccacgg catctttgtt cagcgccagt
    61621 ttttccatca ggctttgacg ttgcgggtga taatgaaccg ctgcgagaac gcccaaaccg
    61681 acagcgccgg attcaccgga gatgatgcgc gggtcgttgc cgtacggatt acccagcacg
    61741 cgcataccta atgcggcaac gctgtcctgg caggagatga attgggtggc gcagttacgt
    61801 aggatttccc agcccagcgg gttaggttcg ccacaggcca ggcctgccat gatggtggcc
    61861 atatcaccgc caacgttgac gatgtcgcct ttgacgccgg agcgataaat acagtcagct
    61921 ttgtcaggtt caacaataat gctgtgcaga ttttgcgggc tatagacgtc gaccagataa
    61981 cccagcacac caccggccat tgctccgaca ccggcttgca gcagaacgtg cgtcggggtt
    62041 acgcccattt cacgcatttg ctcgacggct tcatctgcca gggttgcgta gccttgcatg
    62101 atccaggttg ggattttggt gtaaccttcc catgccgtgt cctgtaccac ttcccagccg
    62161 tgctgctgcg cgtgttgcat ggtcaggcga acggtatcgt catagttcat atctgtgacg
    62221 atgcactcgg caccgaggtt cagaatggcg tcaacgcgtt cctgagcaga acctttcggc
    62281 atgtaaatca ccgcattctg tccgagttgc tgcgctgccc acgccacacc gcgcccgtgg
    62341 ttgccgtcgg tggtggtcgc gaaagtcatt ttttcgccga tggcattttt caggtgctca
    62401 aatgacagcg tttcgatatc aagatgatat ttttcacaca ataattgagc gatagcgtac
    62461 gcaccgccga gcattttgaa ggcgttcaga ccgaatcgtt ttgattcgtc tttgacaaga
    62521 attttcttca ccccaaaaag gtttgcgaga tcgtccagcg cacaaagcgg tgttgggcga
    62581 taaccagcta ttttctggtg gaactggcgc gccagtttgg cctggctttg cgaaaagagc
    62641 ggtgatgttt cgccgttgaa aaatttgtta tcggcgatat caatcttcaa tgagaaaacg
    62701 gacatatcct atccttttgg cctctggaaa tagagggccg accagctgat cggcccgagg
    62761 ggttatttca cgcgttcttg cgcttctttc agcagttgct cgagcagtgc acctggtttg
    62821 gcgtatttac gggacaggat catcgcggcg atgatataag gcttccagct ggcttctttg
    62881 taggtagcga tacggtattt ttcgaatacg ccttcagtca cttccccttc tttgcaggat
    62941 acgccgctga tatcagctgg caggcagtgc atgtacaggg cttcgccatc acgggtcagt
    63001 tccatcatct cttcagtaca atgccagtct ttgtgttgcg cgttctgtgc cagacactgt
    63061 ttttccagtg cttttaagcc ttcgtgatcg ttagcgcgca gcaattcggt acgctgttcc
    63121 atcactttgt aaggtgccca tgacttcgga taaacgatgt ctgcatcttt gaacgcttct
    63181 tccatgctgg tgacctgacg gaagctaccg ccagatgctt tagcattgtt tttcgccact
    63241 tcaaccacat ccgggatcag gtcgtagcct tccggatggg ccagggtgac atccataccg
    63301 aagcgagtca tcagaccgat gatgccttgt ggtacagaga gcggtttgcc atagcttgga
    63361 gagtaggccc aggtcatggc gattttttta cctttcaggt tttccagtga accaaagtgt
    63421 tcacgtaacc acgcgaggtc agccattgac tgagtcgggt ggtcaatatc gcattgcagg
    63481 ttcactaaag ccggacgctg tggcagtaca ccctgcttgt aaccgtcatc aagtgcagcg
    63541 ccaacttcac gcatataggc gttgcctgcg cccagataca tatcgtcgcg aataccaata
    63601 gcgtcggcgc agaaggagat catattggcg gtttcacgca cggtttcgcc gtgagcgatt
    63661 tgtgatttgc cttcgtcgag atcttgttgt gcaaggccga gcaggttaag cgcggaagca
    63721 taagagaagc gggtacgggt ggagttgtcg cggaatacgg aaatacctaa tccactatta
    63781 aagactttgg ttgagatgtt ttcagcacgc agtgctttta atgctgcggc aacgtccagt
    63841 acttgtttca gttcatctgg cgtctgttcc cacgttaaca aaaaatcttt ctcgtgaagg
    63901 tgagaggtca gcgaattgat atccttaatc agctcattaa cagttttcat cataaagtcc
    63961 cggtagtgga atgacaggca aagtggttta tttcactgtg acttgccaaa gtgattggtg
    64021 ttttcacagc tatacctggg actaacaata agtgtgccag aattatccgc gcggctgcaa
    64081 aagactaatt tgacggataa tgtgtggggt tgatcacaaa ttgatgccag cttctggttg
    64141 tttgtaaaat acgaaaacgt ttggcttttt ttatcgcgtt atttatttgc tgctaagtat
    64201 cagaaataag ataaaggggc aggctatctc tcttcagttt gataaactca ccgccgtaat
    64261 tctcatattg atatagaaat gtgatatctc ttccataatt tcattagaaa atgataaaaa
    64321 tcttatgttt ttatcaaagc caatttcgcc tgagttaata tcatcatgat ctgtgaacca
    64381 tcaacgtctt caatgatcga cttttaagta agtctggaag gtaaagctat ggagcttgct
    64441 actacgcagt cagtattgat gcaaattcaa ccgacaattc agcgttttgc cagaatgctt
    64501 gccagcgttt tgcagcttga ggttgagatc gttgatgaaa acttgtgtcg cgtcgccgga
    64561 acgggcgcgt atgggaagtt tcttggtcgc cagttgagcg gcaactcacg cctgctccgc
    64621 cacgtcctgg aaacgaaaac tgaaaaagtt gtgacacagt ctcgcttcga tcccctttgc
    64681 gaaggttgcg atagtaaaga aaattgccgc gaaaaagcat ttctgggtac gcctgtcatt
    64741 ttacaggatc gttgtgttgg ggtgataagt ttgattgccg ttacccacga gcaacaagag
    64801 catatcagtg ataatttacg cgaattttct gattatgttc gccatatatc caccattttt
    64861 gtttcgaaac ttctggagga tcaggggcca ggagataaca tcagtaaaat attcgcgacc
    64921 atgatcgata atatggatca gggcgtatta gttgttgatg atgaaagtcg ggttcagttt
    64981 gttaatcaga ctgccttaaa aacacttggt gttgtacaaa ataatattat tgggaaacct
    65041 atccgtttca gaccattaac atttgagagt aattttactc atggacatat gcagcatatt
    65101 gtttcgtggg acgataaaag tgaattaatc attggtcaat tgcataacat tcagggccga
    65161 caattatttt taatggcatt tcaccaatcg cataccagtt tttctgtagc aaatgcacct
    65221 gatgaaccac atattgaaca attggttggc gagtgccgtg ttatgcggca attaaaacga
    65281 ctcattagcc gtattgcacc cagcccatcc agcgttatgg tggttggtga aagcggcacg
    65341 ggtaaagaag tcgtcgcccg agcaatccat aagttgagcg gaagacggaa taaacccttt
    65401 attgctatca actgtgccgc gattccggag cagcttctgg aaagcgaact gttcggttat
    65461 gttaaaggcg catttactgg cgcttctgcc aacggtaaaa cagggttgat tcaggcggcg
    65521 aatacgggca cgctgtttct cgatgaaata ggtgatatgc cattaatgtt gcaggctaaa
    65581 ttactgcgcg ctattgaggc gcgtgaaatt ctgccgattg gtgccagtag cccaatacaa
    65641 gtcgacattc gcatcatttc tgcaactaat cagaatttgg cccagttcat tgccgaaggt
    65701 aaattccgcg aagatctctt ctaccgactt aatgttatcc cgataactct gccaccgctg
    65761 cgtgaacgtc aggaagatat tgaactattg gtgcattact ttttacatct gcatacccgt
    65821 cgtctgggat cggtttatcc tggcattgct cccgatgtcg tcgaaatatt gcgtaagcat
    65881 cgttggcccg gaaacctgcg cgagttaagc aatttgatgg aatatctggt taacgtagtt
    65941 ccttcaggtg aagttatcga cagcacgcta ttgccgccaa atctgctgaa taatggcaca
    66001 acggagcaaa gtgatgtaac agaggtcagt gaggcgcacc tgtcactcga tgatgcgggc
    66061 ggcacggcgc tggaggagat ggaaaagcaa atgatccgcg aggcgctttc acgtcataac
    66121 agcaagaagc aagttgctga tgaactgggc atcggcattg ctacgctcta tcgcaagatt
    66181 aagaaatatg agttgttaaa cacataacct cagtgaatcc ggcctgaatt caggccggat
    66241 atccttttac ttcgttttct cgcaatccag aactgtattt acaatcatct gataccccgt
    66301 gcagcgacaa agatttcccg ccagtccgcg acgaatttcc gtaatggtta atggcttctc
    66361 gcgtggtttc gccagcattg ccgtggtagc cataatcagg ccaggcgtac aaaacccgca
    66421 ctgcactgcg ccggattttg cataagcctg ctgaacatga gaaagttttc cgcctttcgc
    66481 ttcaccttcc agcgtgcgga tctcttttcc ttcagcccag gcggcaaggt ataagcaact
    66541 gtctattgct gtgccgtcga ccaacaccgt acaggcacca cattcaccca cgcagcaccc
    66601 ttgtttgaca cttagcagtc cttgttcgcg gagtaattcc gagagcggcg tgcctggtgc
    66661 ggcgtgaagc tgaaaaggca tcccgttaat ggtgcattcg atggtaattg tttcgctgtg
    66721 attcattgca attttccccc cgccgcggcg acggcttcgc taatcacttt tttggtcatc
    66781 gtctggatga gatgcagacg aaactcttta ctggcccgcc atgaagaacg cggggcgaca
    66841 tcttgcagga cagattcgct gatagcttcc agcgtttgca ggtttaatgg cgcattttgt
    66901 gcagtctgtt cggcatgttg gcagcgaatc ggcgttggcg cggcaacacc aaatgccagg
    66961 cgtaattcgc tgaaattgcc gttatctagt cggcaatgtg cggcgcagcc aatcgttgaa
    67021 atatccatcg cgtcgcgcat ggcatattta aaatgcgcgc tgcccgcgtg ttctttcggc
    67081 tgtggcggaa aatgaaaggc gacgaggatt tcgtcatgct caagagacac tttgcccggc
    67141 ccggtgtgaa agccattaat cgggacgaaa cgaacaccgc gcggggagtg gatctccagt
    67201 ttcgcgtcat aaattagcgt tggcgtggca gaatctgcgc tggtggcacc gttgcaaata
    67261 tttccaccgt aggtagcgac gttacggatc tgcggtccag caatggacgt ggccgcagca
    67321 cataacgccg ggagatgacg ttgagttata ggatcttcta ttagctgggt aaatgtcgtt
    67381 gcagagccga tacgtagcga gccatcttcc gccagcgtaa ttccccgcag ctccgccaga
    67441 ttatgaatat caacaatatg gcgataacgg tcattgtgat ggtggagctg aatcagtacg
    67501 tcagtgccac cggcgagcag tttggcctgc gggttgtcag ccagcaggtt gatggcatcg
    67561 gcaagggttg ctgcgcgatg gtaagaagca aaatcaaaca tgatgttatc ctcaaatcaa
    67621 tcctgccaga tggaactctt catataaccg ttttggcgtc agcggcagtg tattgattgc
    67681 aacaccggta gccatcttca ccgcgttacg aatagcagcg gcaacaggaa ttattggtgg
    67741 ctcacccagt gacttatgtc cgtatgcgga ttgcggctca ttgatttcga cgaacgcgct
    67801 ttccagttgt ggcagatccg gcatggtcgg cattttgtaa tccagcagat tggggttacg
    67861 gaccacgccg cttttagcat cgatgatcat ctcttcaaat agcgcccagc caatgcccat
    67921 tcccattccg ccgtgtacct gaccttctgc cagcagtgga ttaagaatat gccctgaatc
    67981 atgaacgttg aggatgcggt tgatggtgac tttgcacagc gcaatatcga ccgtcagatc
    68041 aacaaaggta cagccaaacg ccggtgggtt agtggtggtt ttgatggagc tttcagcaga
    68101 gagctgcccg ccgcgttcag ggtggtagaa agcgtccatc gccaaatctt ttaacgacat
    68161 taacggctct tccggtcgtt caaccagcac gatatggcct tttatcaggg tcagattcat
    68221 cgctgactga tgtagcatga ctgcggcgtg agcgatgatt ttctctttta ataatagtgc
    68281 cgcactgcgc agcgcaggcg cggcaacata gctctggcgt gaggcaaatg cgccgggatc
    68341 gaacggcgta acgtcggtat cttgagttga aataacgcga acgtcgctga ccggaacccc
    68401 cacggtttct gccaccattt gcgagaagac ggtgtcggca ccctgaccga tttccgtcgc
    68461 gccgctttgc acgttgatgg ttccatcctg attcatcaga aggcgcgcgc ctgctatttc
    68521 tacgccgaca ggccaggtgt tagaggtgta gctaaaacag gcgacgccaa cgccgcggcg
    68581 caaattgcct tgctggttct ggcattctgc acggcgtttt tcccattcaa agattttccg
    68641 gcctttttca agacactccg gcaaccctgc gctgtaaata cgtttgcccg tgagcggatt
    68701 agcatctcct tcgcgggcgg cgttgcgtaa acgaatttca acaggatcaa tacctaacgc
    68761 tgtcgcggcg tcatcaagca tagactcaac ggcaaatacg acttgtggcg cgccataacc
    68821 acgcatcgca ccagccgagg ggaggttggt atagcaggtc tttgaactgt aagcgtaggc
    68881 acaacgagga taaaggtaag cgactttatt cccccccgca gaagcgatgg agtgcccgtg
    68941 agatgcataa gcgccggtgt tagacagaac atccagacta taacctttca atgttccgtc
    69001 gcggttcacg cccatttgcc cgtcaatggt aaaagcgtgg cgggtacggg ttgcgaggaa
    69061 acactcttca cggctaaggg aaactttcac cggaatgccg ccaagcttgc tggtcaggaa
    69121 tgccgccatt ggctcttcca gtacatcctg tttattacca aaaccgccac cgacaaacgg
    69181 tttgatgact cgtacgcatg accagggaat atccagcgcc tgaccaacca cgcggcgaac
    69241 aatgtgcggg atctgggtgc tggaaacgat ggtaattcgc gagtcatcat ccatccacgc
    69301 cagcgatgtt acgctttcca tgtgacaatg ttgaataacg ggggtctgat agtgcccctg
    69361 tacctggtag tcggcggcat cgattgtttg ttggacatta cccgtcgaca tcgtgctttg
    69421 tttcagtaaa ttgccaccgt tatggattgg tgcagcgtct tctgccagcg ccgcttctgg
    69481 cgtggtgata acgggtaatt cttgccactc aatgctgacc aattgcgccg ctttttctgc
    69541 cgtgagttca tcgcgggcca cgacgatggc aacggcgtca ccatgatgac gaacatggcg
    69601 agttagcagt gcgcgatcgg cggtatcgcg cttgttttcg tcaagtgtcc aggcatgccc
    69661 agcagtagca aatggaatat caggcacatc ttcccaggta aaaatcgcca gtacgcccgg
    69721 caaacttctg gcttgttcat cattaatact tacggcataa ccatgtgcga tagggctacg
    69781 tacatatttc gcataacaca tgcccgccat aacataatcg tcagtatatc gtgcccgccc
    69841 ggtgacctta gcaatggcat cgacgcgcat gcatgattca cccgtagcgg ttgcttcccg
    69901 cgcttccata gaaatcccct cgatttgaac atttggttct tatggcaaag atttagtcta
    69961 agcaagcaag atttacgcca gaaacgacga taagcgttag gggaggaagg aagtgtgatg
    70021 acgttaaaaa aacacagata tgatacgaaa caggactctg aatagggaca gtgatacgta
    70081 tcaatatgag tggcgataaa atcaatgtga taaatcagga ttattacgca tcaggatggg
    70141 aaatagggta aaaagtcagg aacttcgata cctctacagc gaatattttg ctgatgatac
    70201 ttccctttgc caaatgaaat taacctcttt ctctattacc acgtttttcc agaagcaaga
    70261 gattgggtat caagaggctg gctgctatga tatggcgtct tgttttttaa gcgaggaaag
    70321 attttgagtg cgggacgcct gaataaaaaa tctctgggta tcgtgatgtt gttatcggtt
    70381 ggactgcttt tggcgggctg ttcgggtagc aaatcatccg atacaggaac gtattccggc
    70441 tccgtttaca ccgtgaaacg gggggatacg ctatatcgta tttcgcgcac cacgggaacc
    70501 agcgtaaaag agctggcgcg actgaacggc atctcccccc cttacaccat tgaagttggt
    70561 cagaaactaa aactgggtgg ggcgaaaagt agcagtagta cacgtaaatc aaccgccaaa
    70621 tcaacgacca aaaccgcatc ggttacaccg tcatcagcgg taccgaaatc ttcctggccg
    70681 ccagtagggc aacgttgttg gttatggcca acgacaggga aagttatcat gccgtattcg
    70741 acagcagatg gcggcaataa agggattgat atctcagctc cacggggtac acctatttac
    70801 gccgcgggtg caggaaaggt ggtgtatgtg ggcaaccagc tgcgtggcta cggtaatctc
    70861 atcatgatta aacacagtga agattacatt acggcttacg cccataatga cacgatgctg
    70921 gtaaataatg ggcaaagcgt gaaggctggg caaaaaatcg ccactatggg gagcacggat
    70981 gcggcatctg ttcgcctgca tttccagatt cgttaccgtg caacggcaat tgatccgcta
    71041 cgttacttgc cgccgcaggg cagcaagcca aaatgctgat ggcgaattaa tcagcagtca
    71101 gcagcgcggc acttgctaag gagagcgtaa ggtttataat gccttacgca tctcgaagcg
    71161 ggcgtagttc aatggtagaa cgagagcttc ccaagctcta tacgagggtt cgattccctt
    71221 cgcccgctcc aatttatctt cacctgatca atctgccatt tttgctgtat ctgattttct
    71281 atattatctt aattcaatcg cttcagactt ttcgcaatcg ccgtaatact tccccatttt
    71341 gttgtaaata aaaagcgttt acgcaatgca atccttttgt aacaacccag gtaaaacacc
    71401 ttattttcaa gttgttaaat aagtggttat ctacgctttt aacaaacgat tgatggaata
    71461 ttttatgaaa gggaaatctg cattaactct tctgctcgct ggtatatttt cgtgcggcac
    71521 atgccaggcg acaggggcag aagtgacgag tgaatctgta tttaacattc ttaattccac
    71581 gggggcagca acagataaaa gttatttatc gttaaatcca gataagtatc ctaattatcg
    71641 tcttttgatt cactcagcca aattacaaaa tgaaataaag agccactata ccaaagacga
    71701 aattcaagga ttgctaacat taacagaaaa cacacgaaag ttaacgttga cggagaaacc
    71761 ctggggaacg tttattcttg cttctacttt cgaagatgac aaaacagcgg cagaaaccca
    71821 ctatgatgct gtctggctga gggatagttt atggggctat atggcattag tatcagatca
    71881 gggaaatagt gtagccgcaa aaaaggttct gctcaccctg tgggattata tgtctacgcc
    71941 cgaccagatt aagcggatgc aggacgtcat tagcaacccc aaacgactgg atggagttcc
    72001 cgggcaaatg aatgttgtgc atatccgatt tgacagcaac tcccccgtga tggcagatgt
    72061 gcaggaagaa ggcaagccgc agctgtggaa ccataaacaa aatgatgcgt taggtcttta
    72121 tcttgatctc cttatacaag caatcgacac tgatacaatt aacgctgaag attggcaaaa
    72181 aggtgaccga ttaaaatcgg ttgcgctgct gattgcttat ctggataaag ccaatttcta
    72241 tgttatggaa gattccggag catgggaaga agatgcccga ttaaacacat cttccgttgc
    72301 actggtgacc tctggactgg aacgtttgtc gaacttactc tcgaagaaag actctgtttt
    72361 tgtctctgat ttactacgcg aagctaaagc caatgaactg gatgaacctc tttcaacaac
    72421 gaggttgaac caccttatag ataaaggtta tgaaagaata acgttacagc ttgatttagg
    72481 gggagaatcc ccaggttatc tggaaaaaga taaacattac agagaagccg atgcggcttt
    72541 acttaatgtt atttatcctg caaatcttgc aaagataaac accagaagaa aagagcaggt
    72601 tctaaaaata gttaaaaaac tagcaggtcc gtatggtata aaaagatacg agaaggataa
    72661 ttatcagtcg gcaaattttt ggttcaatga tattaaaacc gataccgatc aaaattcaca
    72721 tgcgaaaaga gaaaagagct tcattccatc aacagaagca gaatggtttt ttgactcatg
    72781 gtatgcaaaa tcagcagcaa ttgtttataa agaatcaaga aaggaagagt atttgaatga
    72841 ctcagtgcaa tttatgaata gatcattggc ccaaataaca ggcgagaata tgattggtgc
    72901 aaatggcagg agtgtacctg aaatggcact tcccgaaagc tataactata tccacaagtc
    72961 gggaactttg catgaagcgc caagccctat cattccgcta aattggtcga aagcttcaat
    73021 gactctcatg ttgaaagaga tgtccaacct tatcaatgat gagggaatta aatagttttc
    73081 ataatgagtt acagcacaac gttgggtatt gactgagcaa gatgaacgac aaggacaacg
    73141 acatcagcct tatgaggagc aagaaaatga aagcaaattc gaaaacgatc agaaggatga
    73201 aagttaatgt tcggtctgag gaaagtaaat cgaaacactc atacgtttga aactactttt
    73261 caaaattgga aagaaaatgg cgaagatgtt gcattattaa tgcccgaatt tagtgctaaa
    73321 tggttaccga tagcagaaga gagtggcagc tggtctggat gggtattgct tggagaaata
    73381 tttcccttaa tttcatctga gctcgctggg atggctttaa tgccagaaac ggaaaggtta
    73441 ataggggaat ggcttagttt atccagttcc ccgttaaatc taaagcatcc tgaactaaaa
    73501 tataatcgtt tgcacgtagg taaagtattt gatggagtat tgagccctgc tcaaccacta
    73561 ataagaatat gaacagggga attgaatctt tggttagata aagtcacagt ctgccaatac
    73621 ggaaacgctc cagtgttaga caaaaaatca ttatattggt ccattcattt tgtaattgga
    73681 tttagtaaaa catgttacag aagtcttgtt gatattgaag ttggtgatgt tttattaatc
    73741 tcaaataatt tggcttatgc ggttattt
//
LOCUS       DAZKIV010000005        73705 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZKIV010000005 DAZKIV010000000
VERSION     DAZKIV010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73705
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.005"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..385
                     /gene="yjfI"
                     /locus_tag="ACHOT6_000340"
     CDS             <1..385
                     /gene="yjfI"
                     /locus_tag="ACHOT6_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HFP9945626.1"
                     /translation="ALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSLVDNALDLAEITEEYSH"
     gene            404..1102
                     /gene="yjfJ"
                     /locus_tag="ACHOT6_000341"
     CDS             404..1102
                     /gene="yjfJ"
                     /locus_tag="ACHOT6_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFP9945627.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1153..1812
                     /gene="yjfK"
                     /locus_tag="ACHOT6_000342"
     CDS             1153..1812
                     /gene="yjfK"
                     /locus_tag="ACHOT6_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFP9945628.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1830..2228
                     /gene="yjfL"
                     /locus_tag="ACHOT6_000343"
     CDS             1830..2228
                     /gene="yjfL"
                     /locus_tag="ACHOT6_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFP9945629.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2238..2876
                     /gene="yjfM"
                     /locus_tag="ACHOT6_000344"
     CDS             2238..2876
                     /gene="yjfM"
                     /locus_tag="ACHOT6_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFP9945630.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2879..4042
                     /gene="yjfC"
                     /locus_tag="ACHOT6_000345"
     CDS             2879..4042
                     /gene="yjfC"
                     /locus_tag="ACHOT6_000345"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9945631.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4126..5751
                     /gene="aidB"
                     /locus_tag="ACHOT6_000346"
     CDS             4126..5751
                     /gene="aidB"
                     /locus_tag="ACHOT6_000346"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFP9945632.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5868..6143)
                     /gene="yjfN"
                     /locus_tag="ACHOT6_000347"
     CDS             complement(5868..6143)
                     /gene="yjfN"
                     /locus_tag="ACHOT6_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFP9945633.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6292..6621)
                     /gene="bsmA"
                     /locus_tag="ACHOT6_000348"
     CDS             complement(6292..6621)
                     /gene="bsmA"
                     /locus_tag="ACHOT6_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFP9945634.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6803..7552
                     /gene="yjfP"
                     /locus_tag="ACHOT6_000349"
     CDS             6803..7552
                     /gene="yjfP"
                     /locus_tag="ACHOT6_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9945635.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQFGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7549..8304)
                     /gene="ulaR"
                     /locus_tag="ACHOT6_000350"
     CDS             complement(7549..8304)
                     /gene="ulaR"
                     /locus_tag="ACHOT6_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFP9945636.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8412..9476)
                     /gene="ulaG"
                     /locus_tag="ACHOT6_000351"
     CDS             complement(8412..9476)
                     /gene="ulaG"
                     /locus_tag="ACHOT6_000351"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFP9945637.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQVDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMNDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9830..11227
                     /gene="ulaA"
                     /locus_tag="ACHOT6_000352"
     CDS             9830..11227
                     /gene="ulaA"
                     /locus_tag="ACHOT6_000352"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFP9945638.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11243..11548
                     /gene="ulaB"
                     /locus_tag="ACHOT6_000353"
     CDS             11243..11548
                     /gene="ulaB"
                     /locus_tag="ACHOT6_000353"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFP9945639.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11558..12022
                     /gene="ulaC"
                     /locus_tag="ACHOT6_000354"
     CDS             11558..12022
                     /gene="ulaC"
                     /locus_tag="ACHOT6_000354"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFP9945640.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQEAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12036..12686
                     /gene="ulaD"
                     /locus_tag="ACHOT6_000355"
     CDS             12036..12686
                     /gene="ulaD"
                     /locus_tag="ACHOT6_000355"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9945641.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12696..13550
                     /gene="ulaE"
                     /locus_tag="ACHOT6_000356"
     CDS             12696..13550
                     /gene="ulaE"
                     /locus_tag="ACHOT6_000356"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFP9945642.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            13550..14236
                     /gene="ulaF"
                     /locus_tag="ACHOT6_000357"
     CDS             13550..14236
                     /gene="ulaF"
                     /locus_tag="ACHOT6_000357"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFP9945643.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVLEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            14333..14884
                     /locus_tag="ACHOT6_000358"
     CDS             14333..14884
                     /locus_tag="ACHOT6_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9945644.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(14959..15234)
                     /gene="yjfY"
                     /locus_tag="ACHOT6_000359"
     CDS             complement(14959..15234)
                     /gene="yjfY"
                     /locus_tag="ACHOT6_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFP9945645.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDNVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            15561..15956
                     /gene="rpsF"
                     /locus_tag="ACHOT6_000360"
     CDS             15561..15956
                     /gene="rpsF"
                     /locus_tag="ACHOT6_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFP9945646.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15963..16277
                     /gene="priB"
                     /locus_tag="ACHOT6_000361"
     CDS             15963..16277
                     /gene="priB"
                     /locus_tag="ACHOT6_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFP9945647.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            16282..16509
                     /gene="rpsR"
                     /locus_tag="ACHOT6_000362"
     CDS             16282..16509
                     /gene="rpsR"
                     /locus_tag="ACHOT6_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFP9945648.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            16551..17000
                     /gene="rplI"
                     /locus_tag="ACHOT6_000363"
     CDS             16551..17000
                     /gene="rplI"
                     /locus_tag="ACHOT6_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFP9945649.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(17112..18083)
                     /locus_tag="ACHOT6_000364"
     CDS             complement(17112..18083)
                     /locus_tag="ACHOT6_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFP9945650.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSQENQAVILKAAKESMSYMKDLWSEEEKEEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(18136..19446)
                     /locus_tag="ACHOT6_000365"
     CDS             complement(18136..19446)
                     /locus_tag="ACHOT6_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFP9945651.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIP
                     PSNTLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQ
                     AGVAIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPK
                     IFLESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILL
                     IIGTFMDMTPAVLIFTPIFLPVVTELGMAPIHFGIVMVLNMCIGICTPPVGSVLFVGC
                     SVSKLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(19489..19965)
                     /locus_tag="ACHOT6_000366"
     CDS             complement(19489..19965)
                     /locus_tag="ACHOT6_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFP9945652.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(19986..22067)
                     /locus_tag="ACHOT6_000367"
     CDS             complement(19986..22067)
                     /locus_tag="ACHOT6_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP9945653.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQHLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(22064..23605)
                     /locus_tag="ACHOT6_000368"
     CDS             complement(22064..23605)
                     /locus_tag="ACHOT6_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA--acetoacetyl-CoA transferase subunit
                     alpha"
                     /protein_id="HFP9945654.1"
                     /translation="MRKITTAGALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVVQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DALFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHIAQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(23615..24391)
                     /locus_tag="ACHOT6_000369"
     CDS             complement(23615..24391)
                     /locus_tag="ACHOT6_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFP9945655.1"
                     /translation="MSDQPVLFSRAAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTI
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKLLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(24401..25243)
                     /locus_tag="ACHOT6_000370"
     CDS             complement(24401..25243)
                     /locus_tag="ACHOT6_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFP9945656.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(25253..26044)
                     /locus_tag="ACHOT6_000371"
     CDS             complement(25253..26044)
                     /locus_tag="ACHOT6_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFP9945657.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNLWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            26257..26904
                     /locus_tag="ACHOT6_000372"
     CDS             26257..26904
                     /locus_tag="ACHOT6_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9945658.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRLKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(26888..27526)
                     /gene="ytfB"
                     /locus_tag="ACHOT6_000373"
     CDS             complement(26888..27526)
                     /gene="ytfB"
                     /locus_tag="ACHOT6_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFP9945659.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            27745..28365
                     /gene="fklB"
                     /locus_tag="ACHOT6_000374"
     CDS             27745..28365
                     /gene="fklB"
                     /locus_tag="ACHOT6_000374"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9945660.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            28674..30086
                     /gene="cycA"
                     /locus_tag="ACHOT6_000375"
                     /gene_synonym="dagA"
     CDS             28674..30086
                     /gene="cycA"
                     /locus_tag="ACHOT6_000375"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFP9945661.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPIGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVIVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(30131..30793)
                     /gene="ytfE"
                     /locus_tag="ACHOT6_000376"
     CDS             complement(30131..30793)
                     /gene="ytfE"
                     /locus_tag="ACHOT6_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFP9945662.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(30901..31866)
                     /gene="ytfF"
                     /locus_tag="ACHOT6_000377"
     CDS             complement(30901..31866)
                     /gene="ytfF"
                     /locus_tag="ACHOT6_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9945663.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVVGVVIAVRAKPEKPLTESVSES"
     gene            complement(31975..32835)
                     /gene="qorB"
                     /locus_tag="ACHOT6_000378"
     CDS             complement(31975..32835)
                     /gene="qorB"
                     /locus_tag="ACHOT6_000378"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001491469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9945664.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSETDFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNK"
     gene            32924..33304
                     /gene="ytfH"
                     /locus_tag="ACHOT6_000379"
     CDS             32924..33304
                     /gene="ytfH"
                     /locus_tag="ACHOT6_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFP9945665.1"
                     /translation="MSQVSLSQQMKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(33422..35365)
                     /gene="cpdB"
                     /locus_tag="ACHOT6_000380"
     CDS             complement(33422..35365)
                     /gene="cpdB"
                     /locus_tag="ACHOT6_000380"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFP9945666.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            35555..36295
                     /gene="cysQ"
                     /locus_tag="ACHOT6_000381"
     CDS             35555..36295
                     /gene="cysQ"
                     /locus_tag="ACHOT6_000381"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFP9945667.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(36285..36842)
                     /gene="ytfJ"
                     /locus_tag="ACHOT6_000382"
     CDS             complement(36285..36842)
                     /gene="ytfJ"
                     /locus_tag="ACHOT6_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9945668.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            37167..37373
                     /gene="ytfK"
                     /locus_tag="ACHOT6_000383"
     CDS             37167..37373
                     /gene="ytfK"
                     /locus_tag="ACHOT6_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFP9945669.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(37435..38778)
                     /gene="paeA"
                     /locus_tag="ACHOT6_000384"
     CDS             complement(37435..38778)
                     /gene="paeA"
                     /locus_tag="ACHOT6_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFP9945670.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(39101..39739)
                     /gene="msrA"
                     /locus_tag="ACHOT6_000385"
     CDS             complement(39101..39739)
                     /gene="msrA"
                     /locus_tag="ACHOT6_000385"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFP9945671.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            39945..41678
                     /gene="tamA"
                     /locus_tag="ACHOT6_000386"
     CDS             39945..41678
                     /gene="tamA"
                     /locus_tag="ACHOT6_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFP9945672.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            41675..45454
                     /gene="tamB"
                     /locus_tag="ACHOT6_000387"
     CDS             41675..45454
                     /gene="tamB"
                     /locus_tag="ACHOT6_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFP9945673.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGILSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPQGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTEGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            45457..45798
                     /gene="ytfP"
                     /locus_tag="ACHOT6_000388"
     CDS             45457..45798
                     /gene="ytfP"
                     /locus_tag="ACHOT6_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFP9945674.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            46010..46261
                     /gene="chpS"
                     /locus_tag="ACHOT6_000389"
     CDS             46010..46261
                     /gene="chpS"
                     /locus_tag="ACHOT6_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFP9945675.1"
                     /translation="MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            46255..46605
                     /gene="chpB"
                     /locus_tag="ACHOT6_000390"
     CDS             46255..46605
                     /gene="chpB"
                     /locus_tag="ACHOT6_000390"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFP9945676.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(46685..47215)
                     /gene="ppa"
                     /locus_tag="ACHOT6_000391"
     CDS             complement(46685..47215)
                     /gene="ppa"
                     /locus_tag="ACHOT6_000391"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFP9945677.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            47525..48481
                     /gene="ytfQ"
                     /locus_tag="ACHOT6_000392"
     CDS             47525..48481
                     /gene="ytfQ"
                     /locus_tag="ACHOT6_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFP9945678.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            48621..50123
                     /gene="ytfR"
                     /locus_tag="ACHOT6_000393"
     CDS             48621..50123
                     /gene="ytfR"
                     /locus_tag="ACHOT6_000393"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFP9945679.1"
                     /translation="MTTNQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            50137..51159
                     /gene="ytfT"
                     /locus_tag="ACHOT6_000394"
     CDS             50137..51159
                     /gene="ytfT"
                     /locus_tag="ACHOT6_000394"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFP9945680.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            51146..52141
                     /gene="yjfF"
                     /locus_tag="ACHOT6_000395"
     CDS             51146..52141
                     /gene="yjfF"
                     /locus_tag="ACHOT6_000395"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFP9945681.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(52174..53172)
                     /gene="fbp"
                     /locus_tag="ACHOT6_000396"
     CDS             complement(52174..53172)
                     /gene="fbp"
                     /locus_tag="ACHOT6_000396"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFP9945682.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            53348..54721
                     /gene="mpl"
                     /locus_tag="ACHOT6_000397"
     CDS             53348..54721
                     /gene="mpl"
                     /locus_tag="ACHOT6_000397"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFP9945683.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(54804..55355)
                     /gene="yjgA"
                     /locus_tag="ACHOT6_000398"
     CDS             complement(54804..55355)
                     /gene="yjgA"
                     /locus_tag="ACHOT6_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFP9945684.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDALAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            55449..56801
                     /gene="pmbA"
                     /locus_tag="ACHOT6_000399"
     CDS             55449..56801
                     /gene="pmbA"
                     /locus_tag="ACHOT6_000399"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFP9945685.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPADVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            56984..57370
                     /gene="cybC"
                     /locus_tag="ACHOT6_000400"
     CDS             56984..57370
                     /gene="cybC"
                     /locus_tag="ACHOT6_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFP9945686.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMEILNDNLKVVEKADN
                     ASQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(57415..57879)
                     /gene="nrdG"
                     /locus_tag="ACHOT6_000401"
     CDS             complement(57415..57879)
                     /gene="nrdG"
                     /locus_tag="ACHOT6_000401"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFP9945687.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(58037..60175)
                     /gene="nrdD"
                     /locus_tag="ACHOT6_000402"
     CDS             complement(58037..60175)
                     /gene="nrdD"
                     /locus_tag="ACHOT6_000402"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFP9945688.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(60569..62224)
                     /gene="treC"
                     /locus_tag="ACHOT6_000403"
     CDS             complement(60569..62224)
                     /gene="treC"
                     /locus_tag="ACHOT6_000403"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFP9945689.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPIYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            complement(62274..63695)
                     /gene="treB"
                     /locus_tag="ACHOT6_000404"
     CDS             complement(62274..63695)
                     /gene="treB"
                     /locus_tag="ACHOT6_000404"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFP9945690.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(63814..64761)
                     /gene="treR"
                     /locus_tag="ACHOT6_000405"
     CDS             complement(63814..64761)
                     /gene="treR"
                     /locus_tag="ACHOT6_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFP9945691.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPENTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            64946..64999
                     /gene="mgtL"
                     /locus_tag="ACHOT6_000406"
     CDS             64946..64999
                     /gene="mgtL"
                     /locus_tag="ACHOT6_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFP9945692.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            65140..67836
                     /gene="mgtA"
                     /locus_tag="ACHOT6_000407"
     CDS             65140..67836
                     /gene="mgtA"
                     /locus_tag="ACHOT6_000407"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFP9945693.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVVQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(68042..68428)
                     /gene="ridA"
                     /locus_tag="ACHOT6_000408"
     CDS             complement(68042..68428)
                     /gene="ridA"
                     /locus_tag="ACHOT6_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFP9945694.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGSMIITSGQIPVNPKTGEVPT
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(68501..68962)
                     /gene="pyrI"
                     /locus_tag="ACHOT6_000409"
     CDS             complement(68501..68962)
                     /gene="pyrI"
                     /locus_tag="ACHOT6_000409"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFP9945695.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IGNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(68975..69910)
                     /gene="pyrB"
                     /locus_tag="ACHOT6_000410"
     CDS             complement(68975..69910)
                     /gene="pyrB"
                     /locus_tag="ACHOT6_000410"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFP9945696.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(69914..70048)
                     /gene="pyrL"
                     /locus_tag="ACHOT6_000411"
     CDS             complement(69914..70048)
                     /gene="pyrL"
                     /locus_tag="ACHOT6_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFP9945697.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(70329..70724)
                     /gene="yjgH"
                     /locus_tag="ACHOT6_000412"
     CDS             complement(70329..70724)
                     /gene="yjgH"
                     /locus_tag="ACHOT6_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9945698.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(70855..71568)
                     /gene="bdcA"
                     /locus_tag="ACHOT6_000413"
     CDS             complement(70855..71568)
                     /gene="bdcA"
                     /locus_tag="ACHOT6_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9945699.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPGEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            71639..72232
                     /gene="bdcR"
                     /locus_tag="ACHOT6_000414"
     CDS             71639..72232
                     /gene="bdcR"
                     /locus_tag="ACHOT6_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFP9945700.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            72377..72829
                     /gene="tabA"
                     /locus_tag="ACHOT6_000415"
     CDS             72377..72829
                     /gene="tabA"
                     /locus_tag="ACHOT6_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFP9945701.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            72952..>73705
                     /locus_tag="ACHOT6_000416"
     CDS             72952..>73705
                     /locus_tag="ACHOT6_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9945702.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFTQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEK"
BASE COUNT        17758 a        18318 c        19436 g        18193 t
ORIGIN      
        1 ggcgctggcg acggcgctgc aaactgtacc tgaacaaaat attgatgtga caaatagcga
       61 gagcgcatta attattaaaa tgaatgatta tggcgatttg caaatcaata ttctttttac
      121 ttcccgccaa atgattatcg aaacctttat ttgtccggta agtagtatca gcaatccaga
      181 tgaatttaat accttcttat taagaaatca gaagatgatg ccgttgtcat cggtaggaat
      241 ctccagcgtg caacaggaag agtattacat tgttttcggg gcgttatcgc ttaaatcttc
      301 tcttgaagat atcctgcttg agataacctc gctggtagat aacgcattgg atctggctga
      361 aattacagaa gaatattcac actaattaca aggacgggta tttatgggaa ttttaaaaag
      421 tttatttacg ctggggaaat cgtttatctc ccaggcggaa gaatccattg aagaaaccca
      481 gggcgtgcgc atgctggaac agcatattcg tgacgctaaa gctgaactcg ataaagccgg
      541 aaaatctcgc gttgatctgc tggcgcgggt gaaattaagt cacgataagc tgaaagattt
      601 acgtgagcgc aaagccagtc tggaagcccg tgcgctggaa gcgttaagca agaacgttaa
      661 tccgtcgttg attaacgaag ttgctgaaga aatcgcgcgc cttgagaatc tcattaccgc
      721 tgaagagcaa gtgttgtcga atctggaagt ctcccgtgat ggcgtggaaa aagcagttac
      781 agcgacagcg cagcgtatcg ctcagtttga gcagcaaatg gaagtcgtta aagccactga
      841 agccatgcag cgtgcacaac aggctgtaac aacctctacc gttggcgcat cttccagcgt
      901 ttcgacagcg gcagaatcct taaaacgcct gcaaacgcgt caggccgaac gtcaggctcg
      961 cctggatgct gctgcacagt tggaaaaagt cgcagacggt cgcgatcttg atgaaaagct
     1021 ggcggaagcc ggaattggcg gtagcaataa aagtagcgcc caggatgtat tagcaagact
     1081 gcaacgccaa cagggcgagt aatttttttg ccagcctcgc taaaaggctg gcaactattt
     1141 taaggataaa atatgtctgg ttttttccag cgtctgtttg gcaaggataa taagcctgct
     1201 atcgctcgcg gtccgctggg acttcatctc aatagtggct ttacgctcga cacgttagcg
     1261 tttcgtttgc tggaagatga attgctgatt gcgctgccgg gcgaggaatt tacggtagcc
     1321 gctgtcagcc acatcgatct gggcggcggt agtcagattt tccgctacta cacttcaggc
     1381 gatgaatttc tgcaaatcaa taccaccggc ggcgaagata ttgatgacat tgatgatatc
     1441 aagctctttg tctatgaaga gagctacggt atcagtaaag agagtcactg gcgcgaggcg
     1501 atcaacgcca aagtgatggg ggcaatgatc ttaaactggc aggaaaaacg ctggcagcga
     1561 ttttttaaca gcgaagaacc gggaaatatc gaacccgtct acatgctgga aaaagtagaa
     1621 aatcaaaacc atgccaaatg ggaggtccat aattttacca tgggctacca gcggcaagta
     1681 accgaagata cttacgaata tttgctgtta aatggtgagg aatcttttaa cgatctcggc
     1741 gagccagagt ggttattttc gcgcgcgttg ggtgtcgata tcccgctgac atcacttcat
     1801 attattggtt aattacaagg acgtttacaa tgcatatact ggattcactt cttgccttta
     1861 gcgcctattt ttttattggc gtggctatgg tgattatttt tctttttatc tattctaaaa
     1921 ttacaccgca caacgaatgg cagttaatca aaaacaataa taccgcggct tcactggcgt
     1981 tcagtggtac attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga
     2041 gtattccaga ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg
     2101 ctggtgtcag actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag
     2161 caggaatgtt tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga
     2221 catggtaatg gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc
     2281 acggagcgat cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct
     2341 acgcacaaaa atacttaacg ttggcgctaa tgggcggtgc cgcttttttt gtattgaaag
     2401 gttgtggcga tagtggcgat gtcgataacg acggcgacgg aacgttttat tcgacagtgc
     2461 aggattgtat tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca
     2521 aagcggcatt ttatgccgat gttccgaaga atatgactca gcagaattgt cagtctaagt
     2581 acgaaaattg ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt
     2641 tgttaagccg ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat
     2701 cctcatttgc ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgctccg
     2761 gttctggcaa aaaagagtct tactcttcag gcggctttac caccaaaaaa gcgtcaaccg
     2821 tttcgcgcgg cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat
     2881 gctgagacac aacgttcctg tgcgacggga tctggaccag atcgccgcta acaacggttt
     2941 cgactttcat atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac
     3001 tctgcgccag attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct
     3061 tgaggtggtg gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc
     3121 gttgtactgg gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg
     3181 gatggatttt gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac
     3241 gccgacctcg ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg
     3301 cagcggcatt attccgcgtg atgccgatca gtacaatgcc attcaggaac gcctgatttc
     3361 acgctttagc gagctttata gccgggagcc gttttatttc tgctgctgtc aggacaccga
     3421 tgaagacagg actaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc
     3481 gcggtttatc tacattgaag atctcggttt gggcgtcggc ggtgtactga ccgatcttga
     3541 tgataatgtc atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga
     3601 taacggtccg ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat
     3661 cttgagtaat aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct
     3721 tcttgcatcc tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg
     3781 taaaccgatc tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt
     3841 cgttgaccac gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc
     3901 tctgccgcga tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc
     3961 gtgcggaatg gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt
     4021 tccgcattac attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg
     4081 cagtgaccat actaatggtg actgccattg atggagggag acacagtgca ctggcaaact
     4141 cacaccgttt ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc
     4201 gcgctctgcg aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt
     4261 attggtcagc agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtgaat
     4321 ccgcctgaat tattgcgcta cgatgcgcaa ggacgccgtc tggacgatgt gcgttttcac
     4381 cccgcctggc acctgctgat gcaggcgcta tgtaccaatc gggtgcacaa tcttgcctgg
     4441 gaagaagacg ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttgcacgca
     4501 caggttgagg cagggtcgtt atgtccgata accatgacct ttgccgccac gccattgctg
     4561 ttacagatgt tacccgcgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac
     4621 gattctcact tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg
     4681 gaaaagcagg gcggttccga tgtcatgagc aacaccaccc gcgcagagcg cctggaagat
     4741 ggctcttatc ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat
     4801 ctggtgctgg cgcagactac gggcggtttg tcctgctttt ttgtgccgcg ctttttgcct
     4861 gacgggcaac gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct
     4921 aacgccagct gcgaagtgga gtttcaggat gccattggct ggttgttggg gcaggaaggg
     4981 gaaggaattc gtctgatcct gaaaatgggt gggatgacgc gttttgattg cgccctgggt
     5041 agccatgcca tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat
     5101 gtttttggta atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca
     5161 cttcagcttg aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt
     5221 gccgatgcca aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc
     5281 tgcaaaagag gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat
     5341 tgcgaggaga gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa
     5401 ggttccggca atattatgtg cctggatgtg ctgcgcgttc tcaataagca agcgggcgta
     5461 tacgacttat tgtcggaagc gtttgtggaa gtgaaagggc aggatcgcta ttttgatcgc
     5521 gcggttcgtc gtttacagca gcagctgcgc aagccagctg aagaactggg gcgagagatt
     5581 actcatcagc tattcctgct gggctgcggt gcgcaaatgt tgaaatatgc ctctccgcca
     5641 atggcgcagg cgtggtgtca ggtgatgtta gatacgcgcg gcggcgtacg gttgtcagag
     5701 cagatccaga atgatttatt gctgcgggcg acggggggag tgtgtgtgta agcgtatacg
     5761 actgatgcga cgctggtttc gattaactca atgaaatatg cgaaaattgt aggccggaca
     5821 aggcgctcgc gccgcatccg gcattgttca tttgctgtat ataacactca tgcatacagt
     5881 atcgcctgta cgcgccagtt atcaggctgc tggtcttcgt acatggtaat aatgcggtac
     5941 catgacgcac cttgttcatc ggcctttaac gccacgatac gttccacgtc ctgcggatcc
     6001 cctgaaatat tgctaactga gatctgacct atttcattca ggcccgtcac gcagtcagca
     6061 ctggcgaatt ctgcagactg tgccgtggcg ctcgcaggat aagtcactaa ttgcagcgag
     6121 ggtgaggcaa gtaattgttt catcgtcagc tccatttttc gtttccccac atgttgcagg
     6181 gcattccatc gcggtaataa acgcccaggc ttccagccgg taatttattg tgcacaggta
     6241 aacgcatagg gacgcaaagc aatgtaaagc tgactatgta agttgtctga tttatttacg
     6301 gtacaaaatg gcttgtgaat accactgtcc tgtcacgatg gtttcgtcca ccatgacgac
     6361 aacgtaataa tcagcttttg cagcgacagc ttttgctctg atttctgcta atgcgtcatc
     6421 cggagaaccc cgaaccatcg tgcttacgct acctattcgc tgtaaccctt gcgtctggtc
     6481 gcggcgaatc tcttgcggat ggttcgttac tggcggtgct ggctgtggcg taccttgcag
     6541 tgcgctacag gcacttaaca tcaacaccaa taataaactg gcaaaccggt aaataacgct
     6601 attacgtttc ctgctaacca tagtgtagtg ccttattaat ttaactttgg gggaatgttc
     6661 ccgaattgtt acctggtacg cgattttcga atgaaaacgt gacgaaagcg taacccacat
     6721 ctcaacatta tgaactaata ccgccagcag aaacttgtgc taaggatttc ttcatgtcat
     6781 accagacaaa aaggagagac agatgattga aatagaatca cgcgagctgg cagatattcc
     6841 cgttcttcat gcttatcctg tcgggcaaaa agataccccg ttaccgtgcg taatttttta
     6901 tcacggcttt acttcatcca gtctggtgta tagctatttt gccgttgcgc tggcgcaggc
     6961 tggtttgcgg gtgatcatgc cggatgcgcc cgatcacggt agccgtttta gtggtgacgc
     7021 agcgcggcgg ttaaatcaat tctggcaaat cttgctacaa agtatgcagg aattcactac
     7081 tttacgtgcg gcaatagccg aagaaaactg gctgcttgat gaccgtctgg cagtcggtgg
     7141 cgcgtcgatg ggcgcgatga cggcactggg aattaccgct cgccacccca cggtgaaatg
     7201 taccgccagc atgatgggat cgggctattt tacatcgctc gcccgttcac tgtttccacc
     7261 gctgatacct gaaacggcag cacagcagaa tgaattcaat aacattatcg cgccgctggc
     7321 agagtgggaa gcgacaaacc acctggaaca atttggggac agacctctac tgctgtggca
     7381 tggcctcgac gatgacgttg tgcctgccga cgaatcacta cgtttgcagc aggccttaag
     7441 cgagacggga cgggataaac tgctaacctg ttcatggcag ccaggcgtgc gtcaccgcat
     7501 tacgcctgag gcgttagatg ctgccgtgac atttttccgc cagcatcttt aaacacgcag
     7561 aatgctgacc ccttgcgctt ccagttgttg caggatttcc gggttagcat ttttgccggt
     7621 gataagcata tcgatttgat cggcacggct aaaaagcatt cccgcgcgtt cgccaatctt
     7681 actgctatca accagtacca ccagtttccc taccacgctc agcatcttct gctctgccat
     7741 tgccgtcagc atatcggttt tatacaaccc ttctgcggtc agtcctttgc cgctggtaaa
     7801 catccagtgc ccggcataga gactgttttc gctgccctgc gggcttaaag tgatggactg
     7861 acttttgttg tactgtccgc ccataatgat cacgctgtca tgttcttgat cgatcaggta
     7921 atttgccagc ggtagataat tagtgatgat ttgcactggc ttgccacaca tttcccgccc
     7981 aagcagaaac gcggtggagc cgcagttgat gactacgctt tcgcccggat taaccagctg
     8041 cgaggccgct ttagcaatac gtactttttc atcgtgattc tgcgcctgat gcagattcat
     8101 cggcgtccag cgcgggcgct gttgggtaat agcttctgcg ccattgcgca cctttttcag
     8161 tttgccgctt tcgtcaagtt tattgatatc gcgtcgcgca gtggcaggtg aaattcccag
     8221 acgctcaacg actttctcaa cggtcacaaa gcccaattgt gcgagcattt ccaggaggat
     8281 ttgatgtctt tgtgcttcag tcatgagcta ttccgataaa atttgatttt tttagatgat
     8341 atttgaaata gccaggaaat actacgccgg atagcgcgaa cttctccacg ctatccggta
     8401 taaacgacag attacaggaa tgacttgaac ggcagatccg gttcaatagt gaagcaatca
     8461 tcgaaaccgc gcggatagtg gtactcgaag ttgtctttat ccagcggcca ggtaaatttg
     8521 ccgccaacct gccagataaa cggcttgaag ccatacttca gacgatcttt tttcatctcc
     8581 cacagcacgc ggatctcttg cggatcggcc tggaagtttg accagatgtc gtggtggaac
     8641 gggataacca ctttcgcatt cagggcttca cccatacgca gcatatcggc gctggtcatt
     8701 ttgtcggtaa taccgcgcgg gttctcacca tacgatccta acgccacatc gatctgatgt
     8761 tcgttaccgt gcttcgcata atagttagag tagtgggaat cgccactgtg atatagggaa
     8821 ccgccagggg ttttgaacag gtagtttact gcgcgatcgt tcatgccatc tggcagtacg
     8881 ccagccgctt tttgatcggc aggcagggtg atcagtgcag tacggtcgaa agcgtcaagc
     8941 gcatgaattt caatgtcttt cacttttaca acatcgcccg gtttgaccac gatgcaacgc
     9001 tctttcggta cgccccagcc aatccacaaa tccacacagg ttttcggtcc aatgaacggt
     9061 acgtcatcag cacagttctg catcacagca gcagcgacgt tgacgtcgat atgatcgttg
     9121 tgatcgtgag tcgccagtac cgcgtcgacc tggcgaatcg caaacggatc aagaacaaac
     9181 ggggtggtgc gcaggtttgg ctgcagtttt ttcacgccag ccatgcgctg catttggtga
     9241 ccctgtttca ttaacgggtt accgtgactt tgtttgccag tgccgcacca gaaatcaacg
     9301 caaacgttgg taccaccttc cgattttaac cagatcccgg tgcagccaag ccaccacatc
     9361 gcaaatgtgc caggagcgac ctgttcttgt tcaatttctt cattcaacca gctaccccac
     9421 tccgggaaag tgctcaggat ccaggattca cgggtgatac ttttcacttt actcatcgcc
     9481 atttaccttc atgatagttc aattcgaatc aatatatgat tggttttgat taatcctgac
     9541 actatttttt caggaaggca atgaccattt tttgactttt tactagggaa gttgttgttg
     9601 attttgagta tggaaagatt taatggaatg tgtaatcaat taactaaatg aatttaaatg
     9661 aataattgtt tcgttgtatg aatcccactc tatccatgtg gaattatttg cgggtcgtgt
     9721 cacatttaat cataaataat cttgttgtga ttacttttga aaattagagt gagtgcacaa
     9781 cattccgggt gtgtggaata cccggttacc tcttcttcag gagatcgtta tggagatcct
     9841 ctacaacatc tttaccgtgt tttttaacca ggtcatgacc aatgccccgt tgttgctggg
     9901 tattgtgacc tgtctgggct acatcctact gcgcaaaagt gtcagcgtta ttattaaagg
     9961 cacgattaaa accataattg gtttcatgtt gttgcaggca gggtccggca tcctcaccag
    10021 caccttcaaa ccggtggtgg cgaaaatgtc cgaagtctac ggcattaacg gcgcaatttc
    10081 cgatacttac gcgtcgatga tggcaaccat cgaccgcatg ggcgatgcct atagctgggt
    10141 aggttacgcg gtgttgttag cgctggcgct gaacatctgt tacgtgctgc tgcgtcgcat
    10201 taccggtatt cgtaccatca tgctgactgg tcatatcatg ttccagcagg cggggctgat
    10261 tgccgttacg ttgtttattt tcggctactc catgtggaca accattatct gcacggcgat
    10321 tctggtttcg ctctactggg gcattacctc caacatgatg tacaagccga ctcaggaagt
    10381 gacggacggc tgcggtttct ccatcggtca ccagcaacag tttgcatcat ggattgccta
    10441 taaagttgcg ccgttcctcg gcaaaaaaga ggaaagcgtt gaagacctca aactgccagg
    10501 ctggctgaat attttccacg acaacatcgt ctccacggcg attgtgatga ccatcttctt
    10561 tggtgccatt ctgctctcct tcggtatcga cactgtgcag gcgatggcag gcaaagtgaa
    10621 ctggacggtg tacatcctgc aaactggttt ctccttcgcg gtggcgatct tcatcatcac
    10681 tcagggcgtg cgcatgtttg tggcggaact ctctgaagca tttaacggta tctcccagcg
    10741 cctgatccct ggtgcggttc tggcgattga ctgtgcggct atctatagct tcgcgccgaa
    10801 cgccgtggtt tggggcttta tgtggggcac catcggtcag ctgattgcgg ttggcatcct
    10861 ggtcgcctgc ggttcctcga tcctgattat tcccggcttt atcccgatgt tcttctccaa
    10921 cgccaccatc ggcgtgttcg ctaaccactt cggcggctgg cgtgcggcgc tgaagatatg
    10981 tctagtgatg gggatgattg aaatctttgg ctgcgtctgg gcggtgaaac tcaccggtat
    11041 gagcgcctgg atgggcatgg cggactggtc gattctggca ccgccgatga tgcagggctt
    11101 cttctccatc ggtatcgcct ttatggccgt catcattgta attgcactgg cttatatgtt
    11161 cttcgctggc cgcgcactgc gcgcagaaga agatgcagaa aaacaactgg cagaacagtc
    11221 tgcttaataa ggagttttga ttatgaccgt acgtattctg gctgtgtgtg gcaacggaca
    11281 aggcagttct atgatcatga agatgaaagt ggaccagttt ttaacccaat caaacattga
    11341 ccacacggta aatagctgcg cggttggcga atacaaaagc gagttgagcg gcgcggatat
    11401 catcatcgct tctacgcaca ttgcgggcga aatcaccgtg accggcaaca aatacgtggt
    11461 tggcgtgcgc aacatgctct ctcctgccga ttttggcccg aaactgctgg aagtgatcaa
    11521 agcgcatttc ccgcaggatg tgaagtaagg acgcgccatg aaattacgtg attcgctggc
    11581 ggagaataaa tccatccgcc tgcaggctga agctgagaca tggcaggagg cagtaaaaat
    11641 cggcgttgac ctgctggtgg cggcggatgt tgtcgagccg cgttactacc aggcgattct
    11701 ggatgcggtt gaacagcatg gtccgtactt tgtgctcgca ccaggcctgg caatgccgca
    11761 cggtcgcccg gaagagggcg ttaagaaaac cggttttgcg ctggtgacgc tgaaaaagcc
    11821 gctggagttc aaccacgaag ataacgaccc ggtggatatc ctcatcacca tggcggcggt
    11881 cgatgccaac acgcaccagg aagtgggcat catgcagatc gtcaacctgt ttgaagatga
    11941 agaaaatttc gaccgtttac gtgcctgccg taccgagcag gaagtactgg atctcattga
    12001 ccgcaccaac gcggcagctt aagaaggaat tgaagatgtc attaccgatg ttgcaagtcg
    12061 cgctggacaa ccagactatg gatagcgcct acgaaaccac tcgcctgatt gccgaagaag
    12121 tcgacattat cgaagtgggc accattctgt gcgtgggcga aggcgtgcgt gcggttcgcg
    12181 atctgaaagc gctctacccg cacaaaatcg tgctggcaga cgccaaaatt gccgatgcgg
    12241 gcaaaattct ttctcgtatg tgcttcgaag ccaacgctga ctgggtgacg gtaatttgct
    12301 gtgcggatat caacaccgcc aaaggcgcgc tggacgtagc aaaagagttt aacggcgacg
    12361 tgcagatcga actgaccggc tactggacct gggaacaggc gcagcagtgg cgtgacgcag
    12421 gcattcagca ggtggtttat caccgcagcc gtgacgcgca ggccgcaggc gtagcgtggg
    12481 gcgaagcgga cattaccgcg atcaaacgtc tttccgatat gggcttcaaa gtcaccgtca
    12541 ccggtggcct ggcgctggaa gatctgccgc tgttcaaagg tattccgatt catgtgttta
    12601 tcgctggtcg cagcatccgt gatgccgctt ctccggttga agctgcacgt cagttcaaac
    12661 gttccatcgc cgaactgtgg ggctaaggag cggatatgtt gtccaaacaa atcccgcttg
    12721 gcatctatga aaaagcgctc cccgccgggg agtgctggct ggaacgcctg caactggcaa
    12781 aaacgttggg cttcgatttt gtcgagatgt cggtagatga aacggacgag cgcctgtcgc
    12841 gcctcgactg gagccgcgag cagcgtctgg cgctggtgaa tgcgattgtt gaaaccggcg
    12901 tgcgcgtgcc gtccatgtgc ctttctgctc atcgtcgttt cccgctgggc agtgaagatg
    12961 atgcggtgcg ggcgcagggg ctggagatta tgcgtaaagc tatccagttc gcccaggacg
    13021 tcggcattcg cgtgatccag ctggcgggct atgacgttta ctatcaggaa gccaataacg
    13081 aaacgcgtcg tcgtttccgt gacggcctga aagagagcgt tgagatggcg agccgcgcgc
    13141 aggtgacgct ggcgatggag atcatggatt atccgttgat gaactccatc agcaaggcgc
    13201 tgggttacgc gcactatctc aacaacccgt ggttccagct ctacccggat atcggcaacc
    13261 tgtcggcgtg ggacaacgat gtgcagatgg agttgcaggc cggaatcggg catatcgtcg
    13321 cggtgcatgt gaaagacacc aaaccgggcg tcttcaaaaa cgtgccattt ggcgaaggtg
    13381 tggtggattt cgaacgttgc ttcgaaacgc tcaaacagag tggctattgc gggccgtacc
    13441 tgattgagat gtggagcgaa acggcggaag acccggcggc agaagtggtg aaagcgcgtg
    13501 attgggtgaa agcgcgcatg gccaaagcag gcatggtgga ggcggcataa tgcaaaagct
    13561 aaaacagcag gtgtttgaag ccaacatgga tctgccgcgc tatgggctgg tgacctttac
    13621 ctggggcaac gtcagcgcta tcgaccgcga acgcgggctg gtggtgatca agcccagcgg
    13681 cgttgcctac gaaaccatga aagcagacga tatggtggtg gtcgatatga gcggcaaggt
    13741 gttagaaggg aagtatcgcc cgtcttctga caccgcgacg catcttgaac tctaccgtcg
    13801 ttacccgtcg ctcggcggca ttgtccatac ccattccact catgccaccg catgggcgca
    13861 ggcggggctg gcgatcccgg cgttaggcac cacgcacgcc gactacttct ttggcgacat
    13921 cccgtgtacg cgtggattaa gcgaagaaga ggtgcagggc gagtatgaac tgaacaccgg
    13981 caaagtgatt atcgaaacgc tgggtgacgc cgagccgctg catacgccgg gaattgtggt
    14041 gtatcagcac gggccgttcg cctgggggaa agatgcccac gacgcggtgc ataacgcggt
    14101 ggtgatggaa gaagtggcga aaatggcgtg gattgcgcgc agtattaacc cacaactgaa
    14161 tcacatcgac agcttcctca tgaataaaca cttcatgcgt aagcacggtc ctaacgccta
    14221 ttacgggcag aagtaagcag tgcgccccgg aattattacc tcctgcggga tgtaattggt
    14281 ctgattccgg ggccgcttca gttaaaaaat cgtataaaca acaggtgttt ttatggctat
    14341 caatggattg tttgtcagaa cggatccgca acgtcgccgc tatggcgttg cgttacttgt
    14401 gggtgtactt tcaggaatta tttcagcatt tgttaaatgg ggagcggaag tccccttgcc
    14461 accgcgcaca ttttccggtg gtcgcgatga atttaacccg ccgtatatct ttctacggga
    14521 ttatttaggt atcgacccaa cccaaactgt gtatacattc tcggaacata ttatcaaccc
    14581 ggtgatggtg acgcatatca tcttctcact ggtttttgcg atagggtatt gtattgtcgc
    14641 ggaaattttc ccgaaagtga aattatggca ggggatatta gcagggctta ttgtgacagt
    14701 ggcagtccac ggtatatttt gcccggcgct gaatttaacg ccgccgctca ctcaattgcc
    14761 ttttgacgaa tatgcctctg aaatcctggg gcatattttc tggttctgga taatagaaat
    14821 tatgcgccgc gacttacgta atcgcattac tcatgagcca gatccggaag tcgcaatccg
    14881 gtgaataacc gtatacaaaa gccccctgcg aagggggcaa agcaaactat ggcaatgttt
    14941 cgttggttat acctggtgct agcgataaat atccgcgctg gcgtgcatat tgccgttact
    15001 ccccggttcc cgcatcagaa ttacgtggta gtacgttgcg ccctgcgcat ctgtctcttc
    15061 atttaatgcc tgacgtgctt cgctttcagt ggcgaaatta tgattgatat aaatcacgcc
    15121 taagctttgc acattatcca tgtttctggc ctggtggtta ttaatttcaa tggctgccca
    15181 tgtatttgca cttagcaaaa gcacagccag aagggctaaa acacgactga acatagatac
    15241 ctcctcgacg gctgactttg tgtgctctcc tctgtgatga tcttctgatt taattttaat
    15301 caatgataaa gaagttgatg gtgaccattt ctgatgcagt tgttcaaaaa aacaccatga
    15361 tgaagtgtga tgaactccaa atcagcggtt tagaggttaa ttgcgaaagg ggagatttat
    15421 ttcggctctg cccttgagtt tagcggggca tacaagtact ataacgcgtc atttttcagc
    15481 cgacctttaa cacgttcctt gcctccccgg gattcggctg acccagacag gaggctgaat
    15541 aatccgtaag gagcaattcg atgcgtcatt acgaaatcgt ttttatggtc catcctgacc
    15601 agagcgaaca ggttccgggc atgatcgagc gctacactgc tgccatcact ggtgcagaag
    15661 gcaagatcca ccgtctggaa gactggggcc gccgtcagct ggcttacccg atcaacaaac
    15721 tgcacaaagc acactacgtt ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc
    15781 tggaaactac cttccgcttc aacgatgccg ttatccgcag catggttatg cgtaccaagc
    15841 acgctgttac cgaagcatct ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg
    15901 atgatttcgc aaacgaaacc gctgatgatg ctgaagctgg ggattctgaa gagtaatttc
    15961 tgatgaccaa ccgtctggtg ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca
    16021 gtccatcagg aattcctcac tgccagttcg tgcttgagca tcgttctgtg caggaggaag
    16081 ccggctttca ccggcaggca tggtgtcaaa tgcccgttat tgttagcgga cacgaaaacc
    16141 aggccattac tcacagtata acggtcggca gtcgcataac cgttcagggg ttcatttcat
    16201 gccacaaggc aaagaacgga ctgagcaaaa tggttttgca tgccgagcag attgaattga
    16261 tagattctgg agactagcca tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc
    16321 accgcggaag gcgttcaaga gatcgactat aaagatatcg ctacgctgaa aaactacatc
    16381 accgaaagcg gtaagattgt cccaagccgt atcaccggta cccgtgcaaa ataccagcgt
    16441 cagctggctc gcgctatcaa acgcgctcgc tacctgtccc tgctgccgta cactgatcgc
    16501 catcagtaat cggtcacggt ccattaatac gactttgaga ggataaggta atgcaagtta
    16561 ttctgcttga taaagtagca aacctgggta gcctgggtga tcaggtaaac gttaaagcgg
    16621 gctatgctcg taacttcctg gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca
    16681 ttgaattctt cgaagcacgt cgcgctgaac tggaagctaa actggctgaa gttctggcag
    16741 ctgctaatgc tcgcgctgag aaaatcaatg cacttgaaac tgttaccatc gcgtctaaag
    16801 ctggcgacga aggtaaactg ttcggttcca tcggtactcg cgacatcgct gacgctgtaa
    16861 ctgcagctgg cgttgaagtg gctaagagcg aagttcgtct gccgaacggc gttctgcgta
    16921 ccactggcga acacgaagtg agcttccagg ttcacagcga agtattcgcg aaagtgatcg
    16981 taaacgtagt agctgaataa ttcgttattc aacgagacgt aaaaacgccg accagtggtc
    17041 ggcgttttgc ttttgtagcg agcacttgca gatatcgata agggccacaa taagcagccc
    17101 tacgaagata attacttagc tgcctgaata tcagccagca ttggggcaag ggctgggttg
    17161 tccttagcga agtttgcgta cattggctgt actttctcga taaacggagc tttatctacc
    17221 tggtagactt tcacgcccat tttatccagt tttgcaaatt cttctttctc ttcctcgctc
    17281 cacaagtctt tcatgtagct cattgattct ttagctgctt ttaatataac tgcctgattt
    17341 tcctggctaa gtttatcaaa cgcagcattg ctcatcagaa ccacatcggg aaccatagtg
    17401 tgttcgtcaa ggctgaagga ttttgccact tcaccgtgac gcatggtagt cagcgccatc
    17461 acgctgtttt ctgccatatc gaccacaccc tgctgtaagg ctgtatacaa ctcgccgtaa
    17521 tccagtggcg tcgggttacc gcccatgact ttgatcattt caactgcgcc agggcttggc
    17581 tggacacgga ctttcatgcc tttcagatcg tctggtttca gtactggttt attgccatag
    17641 aaactgcggg cgcctccatc ataaaaagtc agcccgaaat aacctttgct tttcgttgag
    17701 tcgaggattt ttctcccgat atcgccctgc atgacctgat aataatgatc acgatcgcgg
    17761 aacaaataag gcaggctaaa taatttatag gaattttcaa atgcttccat ttctgccgca
    17821 ttggttttta ccaggggaat agcgccagaa cgaatcagct ccattgtttc tcgttgagta
    17881 cccaatgttc catttggata aatacgaata gtaatatcac cgttagagta ctctttgctc
    17941 tttttcgcaa agaactccat cgctttatga acaggatgag acttatcctg attatgactc
    18001 agttttaatg tcactgccca ggactggctt gcggataaca taagaacagc accgataatg
    18061 ccagcggata cctttatttt catagatgtt ccttttctaa taaatatggc ggcaggtgcc
    18121 gccagtgaat gcgcatcagt agcccagtgc tcttggcaat gccatactga tttgcggaat
    18181 ataggtcacc attgccagca ccaataccat cacggcatag aatggcagca taggtttgat
    18241 aattttatta atcggtaatt tagatacact acaaccgaca aataacacac tgcctacagg
    18301 cggagtacaa ataccgatac acatgttcag taccatgaca ataccaaagt gaatcggggc
    18361 catgcccagt tcggtgacaa ccggtaaaaa tatcggcgta aaaatcagta ccgccggtgt
    18421 catatccata aaagtgccga taatcaacag gataatattg ataataagca ggattattat
    18481 tgggttgtca gaaatagtgt taagcaaatc gagaatcagg aagggaacgt cagcgtttga
    18541 cattgcccat gacataccca tcgaagaacc aattaataac aagacaattg cagtggtaat
    18601 aacggattcc agaaatattt taggtaaatc tttaactgag atttcacgat aaagaaccag
    18661 tgcaaggaaa agggtataaa ttactgcaat tgcggaggcc tctgtcgggg taaaaatacc
    18721 acttaataca ccccccataa ttaaaataat caggaaaata ctggggatag ccatccatat
    18781 cgcaacgcca gcctggcgta aagtcgggcg ttctggcttt ggataaccgc gacgtaccgc
    18841 gataataccg gctattacca tcagtgccag acccagcaaa attcccggaa cataacccgc
    18901 caggaacagt gcggcaatag aagtaccacc actgaccagt gaatagacga ttaacgtatt
    18961 acttggtgga ataagcaaac ccgtcggggc agaggcaacg ttaaccgcag tactgaatgc
    19021 cgggtcgtaa ccctctttaa cctgttgcgg atgcatgacg ccgcccatcg cagccgccga
    19081 agccgccgaa gccacggctg aaccagaaat tgccccgaac agcatattgg cgagaatatt
    19141 acagtgggca agtgagcctg gcagacgacc gccgagaata cgggcaaagt tgattaatcg
    19201 tcgcgctatt cctccatggt tcataatgtt gccagacaag ataaaaaatg ggattgccaa
    19261 caacgtaaaa ctatctaacc cggaggctaa cttttgggaa accacactca atgctgagtt
    19321 gaaatcgagt tgtgtggaaa ttgatgccag tgtcgataaa ccaatggcat aaacaatagg
    19381 tacacctaat gccaacataa aaaagaaggc accaaataac acaataatcg gtaaccagta
    19441 atccataatt tctccattcc tactatggtt ggttgttgtt tgttatcttt agagtggttg
    19501 tttaagttcg gttaaaacca tatctatggt gtatataata ataagtacac cactaatcgg
    19561 gatgctcata tagatatatc ccatttgcca gcctaaagcg ggcgaggtct gacctgaatc
    19621 gaatgtatcc atcgttaata gcccaccacc atataccata atgatggttg cgaagataat
    19681 aacgataatc tgaatacaca aaaataaatg acgtcgggct tttcctttca gcatcatagg
    19741 tagcaggtca atcgccagat gccggttttg ccctgcggtg tatgccccac caaaaagtcc
    19801 aagacaaata aagagcaaac ggctgagttc atctgtaaaa atactgggac tagtaaatat
    19861 aaatcgtgca gccacctgcc acgtcacaca tgccacgatg atcgccaaaa tggtaatgtt
    19921 caatgctgca agcagagcat taatagatct aattatattt cgcatagtga ttaacctttg
    19981 caatatcatt catcctgtgg ctggtaaatt gtcccattaa gaaaaccagg ccagtttttt
    20041 tcaaaccaaa tacctcggat aataggaaac tcacgaacag gtgtttctct gacgtgaaag
    20101 ataccgttgc aataatgaat aagtagttta tcttttttat cctcagtacg ctctgaacga
    20161 aagcgccagg catcacaaaa accatttcgt gtttgcggaa tagaatttcc atccctgtcg
    20221 agaataatac gtgctccccg actaccacca cctgcgtcaa tataatgcgt taattgcgtt
    20281 aatacggcgg cagaagtcag tgccatatgc cgccacataa acagctcggc aacttcaccc
    20341 acatgtttga tagccaatcc atgccgttgg acaaattcgc tcaatagcag ggcatcgcga
    20401 gtggcgcgtc ggactttatc ggcatggcaa ataaatcccg catggtcaga cattcgtgcc
    20461 tgaatttttt ctctcacaac cgacaacggc atcccggtcc cgttatcgtg cgcctgagta
    20521 atgatctctc ttatagaagc cagtgtagga gctaccagct gtgctatatc tccatcaata
    20581 ttccgctttt gcgtgcaacc aataaaacgt gccagacgaa cagcaaaaac ctgcccggca
    20641 ttcaatgctg caccaccagg gcgagtgacg ccgtgtgtgc cagcaacttc ccccacggcg
    20701 aaacagccgg gcagggaggt ttgtccccag atatccactt caatgccgcc attcatatgc
    20761 tgattattca tggcaaactg caaaggctgt gtggtgagat cgtaaccgtg catcttatac
    20821 agcgagatag acagcggatt cattcgttgc agccgttcga tgggcgatgg tgccagagcg
    20881 tcgttatttt ccagatacgc gcgaacgtcg tcgtccagtc gatctaatga gaacggcagg
    20941 tcacccggca ccgcttcagg attgcgattg aaatccataa atacctgacg ccctgattgc
    21001 tgctcttgcg ctactgccat atctaacaag ctggagccaa aatccatcac ccgagtggcg
    21061 tggaacggcc actgatagcc cttacggaaa atgtttgaag ccagctcctg ggtggtgcga
    21121 tagtaatccg cgaggaagtt atactcgtta ccctcagcat ccacggaata gatatacggg
    21181 atcacctgta cataggtgcc ggataaattc cacggaaacg tgctacgcgg cgtgcctata
    21241 ccaaactggc tttcggtcag attggttagc gtcagacctt cctccagcgc cagccccagc
    21301 gagccgaagc atttatgtgg ataaacactg tcgcgataaa gctcgcccgg tccgcccgtt
    21361 gccagtacca cattgggcgc agtcacaatt gccagccccc atgggttatg ggcgcgatga
    21421 ccggtcgcga ggattgcccc tgccacgcgg tcttcgccgt tttcgtcacg ctgatgcagc
    21481 agtttaatca ctgttgcact ggtcaacact ggaatggcga ggtgctgtac ttcttccaac
    21541 agcactttca ccattaacct tgaggtacgc ggcccacacg aggttgcacg cccggcttcg
    21601 tcatggtcgg tttgataacg aagaatcgcg ccatagcgat cttccggtaa ttccagacca
    21661 agatattgca acccgccaag agtgtgcaga gaacctaccg cttcgacata agcggtgtcg
    21721 tgatccatcg ccccaccgct cgccagtgcc tctgccagtt tggtgaagtt gtcgccgttg
    21781 cccgcggtag cggcggtaaa cagtgtctgt ttatcggaac cagaacatgc cgacgtcccc
    21841 atatataacc cggcggtggc gatcagcaca ttttgctggc ggcgtttaag ttccacagct
    21901 gcacgcagcc cggctgcacc actgccgact actagggcat ctgtttgcca gagtgggtaa
    21961 tcaacaccgt ctaccgtgac aacccatgcg ggctctgcct gtgcaaaacc ctttgggaaa
    22021 gtaacgtgcg tcagcgcatc aaggatggtg tcatcaagct gactcatgca tcctccatcg
    22081 gcgtaaacag gctactttcc atcaggcgtg ggtgcttgat aattggacgg aaagccatat
    22141 acggcagaat gtcgcgctca atctccacac ctggtgcgat ttcaattaat tcaacgcctt
    22201 ctgccgttaa ctgaaacacc gcgcgttcag tgatatacaa cacttcctga ccactttgct
    22261 gtgcgtaggc ggcgctaaaa gtgatttttt ccacctgggt aaccagttta gggatttgac
    22321 ctgactgggc gatatgcaag ccatctggtg ttacatcaat cttgctgcct ttggcgtcga
    22381 acgtgccgca gaacaccact ttacgggcgt tttgcgcaat ttcgagaaat ccgccggggc
    22441 caatcagatt gccattgaga tgggagacgt tgacgttacc gtactgatcc atctctccca
    22501 tgccgaggaa ggtgatatcg atccctccgc cactgtagaa ttcgaattga tcgagtgatg
    22561 gaataatggc atcggcgttg cgggcgcagc cgaacagagc atcatccagc atcatgccgt
    22621 tatgcacgcc ttgctcgacg ctcagccata aactttgata aggaacgcct tcgcgcagcg
    22681 ctactcctgg aataccgcca ggaataccaa agccaaagtt ggttgaagcg ccagccacca
    22741 gttcgcgtgc tgcccgacgg gcaatcaggc ggcgcaccgg atgactaacc agttcaatag
    22801 cgtcgttaga actcagacgg cgatgttggc cgctaatggt caggtcgtaa ccaccaagat
    22861 atgtttgcgg ttgttcgcgg tgctcaacga tgccatcgac cagaattcct ggtagcttga
    22921 cccgacgtgg ttcaatggca cctgcttcca gcacatcgcg tacctgtacg aacactttgc
    22981 cgccgctgtt gtgtgctgcc agcgccatgg aatagctatc catatcaatc gcttcttctt
    23041 caaggctgac gttgcctcga ggatcggcat aagtgccacg caaaatcgcg taatccactt
    23101 tgaaagggcg ataacgaagt ttggtttcac catcgatttc aatcagttct accaggtcgt
    23161 cggtagtgca ttcattcgac ttaccgccgc cattgcgtgg atcaacaaac gatcccagcc
    23221 caacgtgggt aaaaagcccc ggacgtccgg cgccgatctc ccgtagcagt gcctgaacca
    23281 cgccaccagg aaagcagtag gcttcaatcg tgttattttt taccagcgcc tgcattttgg
    23341 gcgaccaggt aaagtgcccg gcaataatgc gtttgagcat ctctgcgtgc gcaaaacggt
    23401 tagtgccttt gcagtcgcga tcgccaatac ccagcgagtg gattagcgtc aggtcgcgag
    23461 ggtgtccggt ttgcaggaaa cgggcttcaa tagcagccag aatatggtcg gcttctacca
    23521 taccgccgcc gttaccgcta atagcaatag tcgcaccgtc ctgaatttgc gccgccagtg
    23581 ccccggcggt tgttatctta cgcatacaat ttccttattg attatggaag ctcacgctac
    23641 ttttttcggc aaaggcgcgc gtcgcctgct ggcaatcctg agtggcagac attaatccgg
    23701 ccagtagctg gtgcgggtaa tcggcatgtt tattttccag tgccagaatc agtagcttaa
    23761 tatgcgccag tgccaccgga ccgcattttt ccagctgcgc taactggttc gccatccagc
    23821 tttccacctg ttctttttct accacttcat caattaaatt cgccgcctgg gcttcctgcg
    23881 ccgtcaggcg tttacccagc atcagcatct ggcgaccgac caccggacca acctgattaa
    23941 gcacacgttc aatgcccatc cagccaggca ccatgccgag catcacttcc gggttagaga
    24001 atttcgcgcc ggggcgggca atacgaatat cgcagcacaa ggccagttca ataccaccgc
    24061 cgatggtatg accgttgaga ttcgcaatgg ttaattgggg taagtttcgc aggcgattaa
    24121 aaacgtcatt accgcgttta atccattttc tgcccatatc taatggcgaa taggctgacc
    24181 acgatttaat atcgccgccc gcacaaaaga atttatcgcc ggttgccgtt aattcgacta
    24241 atcgtaatgt ggtgtcgttt tcaatttcat ttaaataatg gtcgagagat tcgatcatct
    24301 cttcgttaat agcatgacat ttatcttcac ggttcaatgt caggcggcag gaagctgccg
    24361 ctctgctgaa cagaacgggt tggtcactca tgatcattcc ttaacaataa tgcgcgatgc
    24421 gatcgacgct aatatccagc gtgctgttaa tatctttctg ccaccagtaa ggcgagaata
    24481 tttccagttc tatagcgcca ttaaatccgg cgttttcaac cagccgacga attgacggaa
    24541 tattgatgac accatctccc ggcattcccc ggtcattgac cagatcggtg gtcggcacta
    24601 accagtcgga aacatgaaac gcgaggatgc gttttccggc acgcaggatc tgactggcga
    24661 gatccggatc ccaccagacg tgataaacat ccaccgccac gccgaggcca aactcaccat
    24721 cgggatcgag ttcatcacac cagtccagcg cctgacgtaa cgtgcacagg caagagcgat
    24781 cggcagctgt catcgggtgc agtggctcca gcgcaattgg cacgccgaca tctttcgaat
    24841 gtggcagcaa ctggcgaatc ccttgtttca cttgttctcg cgcttcatat agatctttag
    24901 tgcccgttgg cagaccgcca accacctgca tataacaggc ggcgttaagc accgctgcgt
    24961 cgtccagcgc ccggcggtta tcatcaatcg ccagtttgcg ttccgccagt gtcggcgcgg
    25021 tgtagtaagt gctacggcac aaaccggaaa cgctcatgtt actggcggca agctggcggg
    25081 tgatttgctg gagatcttca ccctgcaact ccctgcgcca gggggcaatt gcgccaatac
    25141 cgcgtgcggc gcaggcatca ataatggcgg gtagcggcgt tttatagccc agtgtcgcgg
    25201 tattgatact gaataaatcc gggcgttgta acagatctct catgattcag cctcagaagc
    25261 gcggcatact taagccgccg tcgatggcga ccgcctgacc acaggtgtaa atcagtttac
    25321 cttccgccat cgcgcggacg gtggaagcaa tatcagccgg ataaccccaa cgtccccacg
    25381 gcaccagacc tttggcaatc agttcgtcgt aataagccgt tgccggaata gtcatatcag
    25441 ttttgatgag accagggcgc acttcataaa cgccaatttg ttcattacaa aggcgagcag
    25501 caaacagacg agcagcggca gaaacggcgg ttttggcaat ggtgtattcg ccgcgattca
    25561 tcgccagcat gatggcgttg atggaactga cgaaaataat cgagcggtgc ggcagctccg
    25621 cttccggttt cggctgtgcc agtaagcgct tactgaatgc ctgagcaagg aagaacggcg
    25681 cgcgggtgtt aatggcaatg ttttgatcga agctgtctgg ttcgaggtcg aggaggtcac
    25741 cgcgtttttt cactgaaatg cctgcgttgt tgagcaggca gtccagacgt ccccacaggt
    25801 tttgcgccgc gtcgagcatc tcttcatgca aggagagatc gccgacatcg gcagggaagc
    25861 aaatcacttc cacgccttca gctatacatt cttgttgtgt gatatgcagt ttttctacgc
    25921 tgtcggcatc gggaagatcg ttaatcagca ggttaaatcc gccacgagca agctccagcg
    25981 cacagccctt tccaatacca cgagcggctc cggtaatcac ggcaacagga cgagtggatt
    26041 tcatacaata gctctctttt tttgaatatt gtttactaag tgacaattag cttgcgatgt
    26101 gccgcaagaa agatcaatca tctggttggt tgatatgcga tagtgatcac aggacatttg
    26161 ataacttgat gaaagtacaa gtttttatcc tttttactgg agcttctaat taatcgggtt
    26221 aagatagtta tcttgaagag gtcgtgggga agcgttatgt atcttgatac cagcaacgta
    26281 cagagcctga aggaaaagct gctgttatgc gcggttaatg aatttgccga atatggttat
    26341 gaaggggcca gggtcgataa tattgttaag gctgcggggt gtagtaaaca gacggtttat
    26401 catcactttg gtaataaaga aaatttattt attgaagtac tggaatatac ctggaacgat
    26461 attcgactaa aagaaaaggc ccttgatttc tcagatcttc cgccacaaaa agccattgag
    26521 aaaattattg attttacatg ggattattat attgctaatc cctggttttt aaaaattgtg
    26581 catagtgaaa atcaaagtaa aggtgtgcat tatgcaaaat cacagcgtct cttagaaatt
    26641 aaccatgcgc acttgcagtt aatggaatcc ttattggatg aagggaagaa acacaatatt
    26701 tttaagccag atatcgaccc attgcaggtg aatattaata ttgctgcgct tggcggatat
    26761 tatttgatca accagcatac gcttggcctg gtttatcata tcagtatggt ttccccccag
    26821 gcactggaag ccagacgtaa ggtcatcaaa gagacaatcc ttagctggct tttggttgac
    26881 ccttcatcta ccgcgcacga ataaaactgc catccggctg gcgggtgaac aggacctgtt
    26941 gattattccc cgtatcaatg gttaagcccg tcaccacgcc gctggcgttc tggcggattt
    27001 tcaccatttg cccgttttgc aaattactca gcggcttacc cgcgccttct acctgcgcca
    27061 ttgcatagac atcggtagcg ggcagtccgt ggtcgcggaa cagctgcgcc atcgttttac
    27121 cgggttcgac acggtaagag cgccattgat tatcaattcc gctatctggc tggaatggtt
    27181 gtgtttgggt agtttgcggc tgttcttctg gctggccttc ctgtataggt tctggagcga
    27241 ccggagccac ctgatcagga tcattttgtg gtgtgactaa ttgcgcccgc agctgttctt
    27301 ctgtcggcgg ttgtgattgc gactgaatat ctaactgcgc ttcacgcgtc accaccggcg
    27361 cgttgggcgt atcatcagat ggaagcagaa atccgaccac caatacaatg gcggcaataa
    27421 tgatcccccg acgatgcatc ggcggcagcg ggtccatgaa gcgaaaattg tccggcgcgt
    27481 gccagacttt ctccagggtt ggttttagtt caaagcgccc gggcatggtt tcctcctgct
    27541 ccgcgtcttg ttcctcaatc atagcctatg aataagctaa cgctatgatg tccgtggtaa
    27601 acccgccttt attatattca tacgggatat tgctattgtt tctttttccc cgggatttgt
    27661 catcattcct gcgacaaagt tttacccaaa gaagtgtggc tgatatgctg cccgctactt
    27721 ttatacccta agaaaggaaa tacgatgacc accccaactt ttgacaccat cgaagcgcaa
    27781 gcaagctacg gcattggttt gcaggtaggg caacaactga gtgaatctgg cttggaaggg
    27841 ctgctgccag aagcactggt tgcaggtatt gccgatgcgc tggaaggcaa acatccggct
    27901 gttccggttg atgtggtgca tcgcgcgctg cgtgaaatcc acgagcgcgc cgatgccgtt
    27961 cgtcgtcagc gtttccaggc gatggctgct gaaggtgtga aatacctgga agaaaacgcc
    28021 aaaaaagaag gtgtgaatag caccgaatct ggcctgcaat tccgcgtgat caaccagggt
    28081 gaaggcgcaa ttccggcacg taccgaccgc gttcgtgttc attacaccgg taaactgatc
    28141 gacggcaccg tgtttgacag ctccgttgct cgtggtgaac ccgctgaatt tccggttaat
    28201 ggcgtgatcc ctggctggat tgaagcactg actctgatgc cggtaggttc taaatgggaa
    28261 ctgactatcc cgcaggaact ggcatatggc gagcgcggcg caggcgcatc catccctccg
    28321 ttcagcaccc tggtgtttga agtcgaactg ctggaaatcc tctaagcagc gcattctgtt
    28381 cccctcgaac cagaggggag caggcattca gcaataaacc cttcagtttg ccaaacggcg
    28441 ctattttgtg ttgcaaagac cccgtaagcg tgtatttttg tgagctgttt cgcgttatca
    28501 ccgtgatatg acactcactt taaacataaa attaacatta gatctaaatc ttagtattca
    28561 tcccgcgtat tgttacctaa tatcgatgag tcccgataca gattcgtcgt atcatagact
    28621 gactaaaggc cgtagagcct gaacaacaca gacaggtaca ggaagaaaaa aacatggtag
    28681 atcaggtaaa agtcgttgcc gatgatcagg ctccggctga acagtcgcta cggcgcaatc
    28741 tcacaaaccg acatattcag cttattgcca ttggcggtgc cattggtacg gggttgttta
    28801 tggggtctgg caaaacgatt agccttgccg ggccgtcgat cattttcgtt tatatgatca
    28861 tcggttttat gctctttttc gtgatgcggg caatggggga attgctgctt tcgaatctgg
    28921 aatacaaatc ttttagtgac ttcgcttccg atttactcgg gccgtgggca ggatatttca
    28981 ccggctggac ttactggttc tgctgggttg taaccggtat ggcagacgtg gtggcgatca
    29041 ctgcttatgc tcagttctgg ttccccgatc tctccgactg ggtcgcctcg ctggcggtga
    29101 tagtgctgct gctgacgctc aatctcgcca ccgtgaaaat gttcggtgag atggagttct
    29161 ggtttgcgat gatcaaaatc gtcgccattg tgtcgctgat tgtcgtcggc ctggttatgg
    29221 tggcgatgca ctttcagtca ccgattggtg tggaagcgtc attcgcgcat ttgtggaatg
    29281 atggcggctg gttcccgaaa ggtttaagtg gcttctttgc cggattccag atagcggttt
    29341 tcgctttcgt agggattgag ctggtaggta caaccgctgc ggaaaccaaa gatccggaga
    29401 aatcactgcc gcgtgcaatt aactctattc cgatccgtat cattatgttc tacgtcttcg
    29461 cgctgattgt aattatgtcc gtgacgccgt ggagttcggt agtcccggag aaaagcccgt
    29521 ttgttgaact gttcgtgttg gtgggtttgc ctgcggctgc cagcgtgatc aactttgtgg
    29581 tgctgacctc tgcggcgtct tccgctaaca gcggcgtctt ctctaccagc cgtatgctgt
    29641 ttggcctggc ccaggaaggt gttgcaccga aagcgttcgc taaactctct aagcgcgcag
    29701 tacccgcgaa agggctgacc ttctcctgca tctgtctgct cggcggcgtg gtgatgctgt
    29761 atgtgaatcc cagcgtgatt ggcgcgttca cgatgatcac gacggtttcc gcgattctgt
    29821 ttatgttcgt ctggacgatt atcctttgct cgtacctggt gtatcgcaaa cagcgtcctc
    29881 atctgcatga gaagtcgatc tacaagatgc cgctcggcaa gctgatgtgc tgggtatgta
    29941 tggcgttctt tgtgttcgtg atcgtgctgc tgacactgga agatgacact cgccaggcgc
    30001 tgctggttac cccgctgtgg tttatcgcgc tggggttggg ctggctgttt attggtaaga
    30061 agcgggctgc tgaactgcgg aaataaccgc attatcatgc tggatggcgc aatgccatcc
    30121 agcttttaga tcactcaccc gctagggcgc gtgggaacag tacattgttt tccagactga
    30181 tgtgatccat caggtcatca atcagttcat taatgccgtt atacatcgct ttccaggtgg
    30241 tgcaggcttc tggcggcggt gtgacgttat tggtagtgtg tttgatcact tccagcagtt
    30301 cgcccgcttc atcgtgctcg ctttccatta cgctgattgg ccccattgcc tggctgccca
    30361 tgccttgttt gatcatcggg aagaggatct gctcttcttt catcatgtgg ctggaaagct
    30421 cttcatgcag catggtcagg tattttgtca gcccttttgg cacgctcggt ttgtcggcgt
    30481 gaacgcgctc gactttagtc gcttgcagga tcagctccgg cagttgctcg cggtgacgat
    30541 cgtggtagcg cacgatgata tggtcaatga tttctgccag cggggcgcta cgccagtctt
    30601 tctcaatcgg ttgttcagca agctttgcca gttcagcttc aatgacctca acatccagtt
    30661 ctttacgtgc cgccgcgcgc gccagcgtct gcttaccgcc acagcagtaa tccatatcat
    30721 atttacgaaa cagagccgaa gcgcgaggga tagagagcgc cagttcacct aaaggttggt
    30781 cgcgataagc catagctgat acctcattct taataagata agatgtattt taaatgcatc
    30841 tttaaggtga atgctataac ccttacgtag cagaggctta tttaactcac agccagtatg
    30901 tcaactttct gagacagatt cagttaaagg cttttccggt tttgctctga ctgcaatgac
    30961 cacgccaacc actaacagcg cgataccgct cagcgtcatc agcggcggca tttgctggcg
    31021 gagtaaaaag gtgtacagca aacctgccag cgtttcgaaa acaatcagcg gcccgagaat
    31081 cactgtcggt aatcgctggc tggcgacgtt ccagcagagt gcgccaaccc atgagcaaag
    31141 cacggctatc gcaaccatca gactaataaa caccagcgga cgggggccaa aaggtaagga
    31201 gaagtccggc gtttgtatat tcagccagta acaggcgacg agatagccga tgagagaaac
    31261 cggcagcgtg accagcgcct gcgccgtcgc ccacatcatc ggatgtttgt cgggattttc
    31321 ccgcagccag cgggcgttgc gcagggcata ccatgcccag cagaccacgg aaactaacgc
    31381 tagcacgatg ccagaggtat aacgtgccca gtcaaaatcg gggagtccgt ggtttaactc
    31441 agcaatattc acactcgcca ggccgatgcc aatacaaatc agtgccgggg cgagttttcc
    31501 ccacgcgagt ttgccgtcgc gctggctata aagcagattg gcaaagacag gaatgaccac
    31561 cggcagggtg ccgataatca tcgtggaaac aggcgcgcca gtacgttgaa tggcactggc
    31621 aaggcagaag taataaatga ggttgcccat catagtgagc atcaaggcgg taagccagtc
    31681 ccgacgcgcc aactgacgca gacgcacgcg tcccagccag gcaatgggca gcgcaattaa
    31741 ccctaacgcc agataacgcc ccatcgactg caacatcgcc gggtattccg gcacgatcaa
    31801 cggcccgaca aaaataagcc cccacatcaa ccctgctaac agggcgtaca gcacgccgct
    31861 aatcattact ggcatccatt ggtctgtcag aagaaacgtt caggaagcag acatgattgc
    31921 tcccggcatc ccgtcattat cagagaggat gcgggaggat gccactttaa ttaactattt
    31981 attaacatta aaaagatggc ttacgctttc ggctaacgtt gtcgttgggc ggccaatcaa
    32041 tttgctcagc gttttgctgt catcaaacag accgcctttc gatgcgccaa cgtcagaatc
    32101 cgccagcata tccgccagtc cgtcaggcag gccaacgctt ttcagcgccg cagcgaaatc
    32161 ggtttcgctc agattttgat aggtaacctg tttgccgctc tgtttggtca gctctgccgc
    32221 taactgtgtc aacgtccagg cactatcgcc cgccagctcg taaaccttgc cttcgtgacc
    32281 ggcttcgcta atcacgcgtg ccgcagctgc cgcataatct gcccgcgttg ccgaggcaat
    32341 tttgccatcg cccgcggcac cgataaatac gccgtgctcc agtgctgccg gggcgctggc
    32401 gaggtagttt tcgctgtacc agccgttacg cagcagggtg taaacgatgc cagaatcagc
    32461 caacattttc tccgtctcaa tgtgctcatc ggcgaggccg agcggggagg tatctgcatg
    32521 tagcaggctg gtataagcga taaatttcac gccagccgcc tttgcggcat taataacatt
    32581 acgatgctgc ggggcacgtt gacccacttc gctggaagag atcagcagta gtttttccac
    32641 tccctgaagt gcagatgtca gtgcggcttc atcgccgtag tcagcctgac gcacggtaat
    32701 gccttgtgct gccagagcct gggcttttgc cggattacga acgatagcca ctatttggct
    32761 ggcaggaacc gttttcatca aggattcaat aatatagtga ccaagttggc cagtggcacc
    32821 agtaatagcg atcatgggaa gtctccatcg ttttgtcttg tgtttaaata cgctaacacc
    32881 taaacttact tttagtaagt acgtacaaaa aggtaagtat gaaatgagtc aggttagcct
    32941 gtcgcaacaa atgaaagagg gcaatctctt tgcggaacag tgcccgtcgc gcgaggtgtt
    33001 gaaacacgtc accagccgtt ggggggtgtt gattctggtg gcgctacgcg aaggtactca
    33061 tcgctttagc gacctgcggc gcaaaatcgg tggggtgagt gaaaagatgc ttgcgcagtc
    33121 gttacaggcg ttagaacagg atggttttct taaccgtatc gcgtatccgg tggtgccgcc
    33181 gcatgtggaa tatagcctca cgccgctggg cgagcaggtg agcgaaaagg ttgctgcact
    33241 ggcggactgg attgagttga atttgcccga ggtgttggcg gtgcgggatg aacgagcggc
    33301 ataactgccg gatgcgcggc ctacggtgta tgacttgtag ggcggataag atgcgccagc
    33361 atcgcatccg ccaataaatg ccggatgcga ttgatgattt ataggccgaa aaagaagtgt
    33421 tttacttact caaatccacc tgataaatcg caaacccgat atcatcggtc gcgactttat
    33481 tcatcggata ctgccctttc tctttaataa acgctgcggc tttatctgac ggggaagttt
    33541 cgaaacggat atccagtttc ttatcggcag ctatcggtgc taaacgccag ttgttatctg
    33601 ccgccggatg aatttccccc gcacgtttcg actcatcagc aatccacgct gccagcaccg
    33661 agcggttctc atccggtgaa gcaaaagcga tatggctgtc gcccgtaccg gcaaatttgc
    33721 cgccgtaagc gcgatagtta ttggtggcaa cgaggaacat ggcgttcgga tcaatcggct
    33781 tgccgttaaa ggtcaggttc ttaatccttt ccgcattggc attaatcatc tggcactcgc
    33841 cgtcataacg ggcgggttgg gtaacatcaa tctgataatt cacaccatca atcacatcaa
    33901 agttataagt gcggaaacca tcccagttaa tgagtgactg tggtttcgtg ctgttaggat
    33961 caatctggtt aaactgtccg gcagagcatt ccagccactc tttcacctct ttaccgctgg
    34021 ctttcaccac aatcagcgta ttggggtaga gataaagatc ggcggcatta cggaaggtca
    34081 actggccttt ttccacctcc acatagcttg ccgggtcatt tttgcgacca ccaactttaa
    34141 acggtgcggc agctgaaagc actggcagtt ttgccagatc cggatcgccc tgaatgtaat
    34201 gctcgacata cgctttttgc gcgttgttca ccacctgcac ggtcggatcg tcctgcacta
    34261 gtgccagata gctatacata ttgtcggcag atttaccgat tggcttgctg acgaactggc
    34321 gtgtggcatc gtgatcggct ttgagtgttt ctaccagctt gctgtcttcc gccgcgaggg
    34381 attttttatt agcgatgtcg taaatcggtc gtgcttccgc tttcgcctgc gtcacctgcc
    34441 atttaccgct gtcattactg agttgtaagt cgaccacacc aagatgatcg ccccacatgc
    34501 ctggcattac cgccggaaca ccattcagcg tgcctttcgc gatatcagct ccttcgatat
    34561 cagcaaaatc tttacttggg aaaacggcgt gagcatggcc aaacataatg gcgttaacgc
    34621 ccggaatttc actgaggtaa taaactgagt tttccgccat cactttatac ggatcggcag
    34681 acagcccgga atgcgccaga acgacaacga catcggcacc tttctcgcgc atttcaggca
    34741 cgtatttgcg cacggtttcg gtaatatcat tcaccgtcac tttcccggat aaattagctt
    34801 tatcccagcc catgatttgc ggcggtacga cgccaatata gccaatcttc agcgtctgtt
    34861 tttttccgtc tttatcgacc acttcggtgt ctttaattaa atacggtgta aacattggct
    34921 gtttggttct ggcgtcaatg acgttggcat ttacataagg gaatttcgct cccgccagcg
    34981 catttttcag gtaatccaga ccgtagttaa actcatggtt gccaagcgtg ccgacggtat
    35041 agtccagcgt atttaatgcc ttatacactg ggtgaatatc acctgctttt aatcctttcg
    35101 ccgacatgta atctgccagc ggactcccct gaatcaaatc gccgttatcg accagtacgc
    35161 tgtttttcac ttcattgcgg gcatcgttaa tcaggcttgc cgtacgtacc agtccgaatt
    35221 tttccgtggc ggtgtctttg taataatcga aatccatcat gttgctatgc agatcagtgg
    35281 tttccatgat acgcagatcg accgtcgctg cattcacact ggcggcaatc agcgtggcca
    35341 ggagcgttgc gctaaactta atcatcaggg acatcctttt atcatcggga atacgaaaga
    35401 aaagagagaa taaacgtctt acttatagaa cagtgaagaa tgccacaatt ttacgctttg
    35461 aaaatgatga cactatcaca gttggcgcat tcattaacga tagggtataa gtaaaacaat
    35521 aagttaacac cgctcacaga gacgaggtgg agaaatgtta gatcaagtat gccagcttgc
    35581 acggaatgca ggcgatgcca ttatgcaggt ctacgacggg acgaaaccga tggacgtcgt
    35641 cagcaaagcg gacaattctc cggtaacggc agcggatatt gccgctcaca ccgttatcat
    35701 ggacggttta cgtacgctga caccggatat tccggtcctt tctgaagaag atcctcccgg
    35761 ttgggaagtc cgtcagcact ggcagcgtta ctggctggta gacccgctgg atggtactaa
    35821 agagtttatt aaacgtaatg gcgaatttac cgttaacatt gcgctcatcg accacggcaa
    35881 accgatttta ggtgtggtgt atgcgccggt aatgaacgta atgtacagcg cggcagaagg
    35941 caaagcgtgg aaagaagagt gcggtgtgcg caagcagatt caggtccgcg atgcgcgccc
    36001 gccgctggtg gtgatcagcc gttcccatgc agatgcggag ctgaaagagt atctgcaaca
    36061 gcttggcgaa catcagacca cgtccatcgg ctcttcgctg aaattctgcc tggtggcgga
    36121 aggacaggcg cagctgtacc cgcgtttcgg gccaacgaat atttgggata ccgccgctgg
    36181 tcatgctgtg gctgcggctg ccggagcgca cgttcacgac tggcagggta aaccgctgga
    36241 ttacactccg cgcgagtcgt tcctgaatcc ggggttcaga gtgtctattt attaattaat
    36301 ttatgcagca ggtccatcac ttgctgcacc tcttcctgag taagcgcccc gtctttggcc
    36361 cattgcacgc gaccgtcttt atccagcacc actacagcgg aacttttttc atccagctgc
    36421 caggcaccgc gtgcgacgcc attgctatca acaataaact gcgaccaggg ataaagcttt
    36481 ttattactct ccagactgct gcgcacaaac atgccggaac ccggaattgc gtcgtcggtg
    36541 ttaacaatgg tggtggtctg gtaacgatcg tgcggtaact tcgctgattt aatcgcttca
    36601 atcagcgtcg cgtttttctc ttttgcagag gtgcgaccag caatatgttg cagtactcgc
    36661 acttttccca ctaactgcgc gctgttccag gttttgtagc taaactgatc tttatcaagc
    36721 accaactcgc cacgatcggt gatgccaatc ggcggcactc gctgaccggt ttcgaactga
    36781 tgtgcggaag ccatcatcgg caacagcagg caggtgagtg ccagaatctt gcgtagggtc
    36841 atggtgtttc cttcttatga tatgcaggtg atccgaccac ttgggtctga gttttaatca
    36901 tatgtgctat ttatcgaatt tcccgcaagt gtgatgccag tttgcggtca agcgcacaaa
    36961 tcatatgaaa aatgaatgct tatactgaag accgcgcttc ggtaaaaaga taattctgaa
    37021 taattgtaac ctttaggtaa aaaaagttat acgcggtgga aacattgccc ggatagtcta
    37081 tagtcactag gcattaaaat ttgcgcctca taatagttgg gccgattgtg gcaccgcaca
    37141 ggcgtaatac tcagcaggag ataacaatga aaattttcca acggtacaac ccacttcagg
    37201 tggcgaagta cgtaaagatc ctgttccgtg gacggttata catcaaggac gttggcgctt
    37261 ttgaatttga taagggtaag attcttatcc caaaagtgaa ggataagctg catttgtcag
    37321 tgatgtccga agttaaccgt caggttatgc gtctgcaaac agagatggct taaccaaagt
    37381 gctatgcagt aataagacgg ctcctgattc aggagccgtt gatgtttctg ggggttacgc
    37441 gacgctttct tctttatctt tagcgtcagg cagttttggc gaaagggcgg tggccttgct
    37501 gtcgatccgg gtcaccagca gttggtcgat acggtagtta tcgatatcca ccacttcaaa
    37561 tttgtagccg gcgaatttca ccgaatcggt gcgtttcggg atcttacgca gcataaacat
    37621 cataaagccg ccgatggttt cgtagttgcc cgactgcggg aactcgtcaa tatccagcac
    37681 gcgcatgacg tcgtcaatcg gggtgccgcc gtcaatcagc catgagttct catcacgggc
    37741 gacaatctgc tcttccagcc cctgaccgac cagatcgccc atcagcgtgg tcatcacgtc
    37801 gttgagggtg atgatcccca ctaccagcgc gtactcgttc atgatcaccg cgaagtcttc
    37861 acctgcggtt ttaaaacttt ccaacgcttc tgaaagggtt aacgtatccg gcacaatcag
    37921 cgtgttgcga atttgtacgc cgctgttcag tgccaggctt tggttagcca gcacgcggtt
    37981 cagcaggtct ttagaatcga catatccgat gatgtggtca atatcttcat tacagacgag
    38041 aaacttagag tgcggatgtt ccgccacctt attcttcaga ctttgctcat cttcgtggag
    38101 atcaaaccaa atcacgtttt cacgcggtgt cattgaagac ggaacggtac gggattccag
    38161 ctcaaagacg ttttcaatca gctcgtgttc ctgtttacgt aacacgcctg ccagcgcacc
    38221 ggcttccact accgcgtaga tgtcatcaga agtgatgtca tctttacgta ccattggcag
    38281 tttgaaaata cggaagatta tgttcgccag tccgttgaag aaccacacca gcggggtgca
    38341 aacgtacagg cagaagcgca tcgggttgat gatacgcaaa gccacagctt ctggcgcaat
    38401 cataccgatg cgtttcgggg ttaaatccgc aaacaggata aacatgccag tcactaacga
    38461 gaaagagaga ataaagctca gttgctcaga gagctctgcc gacatatagc gggagaacag
    38521 gctgtgaaaa gctggagaaa atgccgcatc accgacgata ccgccgagaa tcgccactgc
    38581 gttcagaccg atttggacca cggtaaagaa catgccggga ttttcctgca tattcagaac
    38641 gcgttgggca tttatattgc cttcatcagc cagcagttta agtttgattt tgcgtgaggc
    38701 ggcaagcgag atctcggaca tcgagaagaa cgcacttaca gcgatcaagc agagtatgac
    38761 taaaatactg tttaacatat cttatccgac ctttcaggcc agatcctcgg aaggggaagt
    38821 tgattaattt gtgtgaaata cacattgaaa gccgattcga agaaagtgaa ccggcatttt
    38881 cagtggctag tatagcgtaa ggtactgtaa agtcgccaga gggttaaaat tcagactgct
    38941 gataaatgca atactgcctg atgcgctatg ccgatcaggc ctacgcagtc cttgcaatat
    39001 attgaatttg gacgattttg taggcctggt aagtcgttta agccgcatca ggcatagaca
    39061 aagagcactt tatcaacaaa ctacatttgt acccgtaacg ctatgcttcc ggcggcagac
    39121 agacgccaat tccgccaatt ccacagtaac catatgggtt tttatgcaga tattgctggt
    39181 ggtcatcttc ggcataataa aacggtgtgg cgttagcgat ttccgtggtg atgtgacgat
    39241 cgtcatcggc ggcaagcatc gccgcctgaa aacgttccag actggcgcga gctgcggcat
    39301 cctgttctgg ggtcagcgga taaatcgctg aacgatactg cgtgccgtgg tcattgccct
    39361 gacgcattcc ctgggcggga tcgtgattct cccaaaatac ctgtagcaac tgctcatagc
    39421 tgatgacgga aggatcgtaa acaatgcgta ccgcttcggc atgacccgta tcaccggagc
    39481 acacttcccg ataagtcgga tttggcgtat agcctccggt atagcctgcg gcggtgctgt
    39541 aaacgccggg taactgccag aacagacgct ccacacccca gaaacaaccc atcgcaaaaa
    39601 tggcaatctc cattccgtca ggtacattgg tcattgagtg accgttgacc gcatgcagcg
    39661 tggctacggg catcggggtg ttacgtccag gcagggcatc ggcgggggaa accagatgct
    39721 ttttatcaaa taaactcatg gtgtcgctct cccgaaaatc ggtcattggg gttaaggttg
    39781 taacaagagg cgtatttgca cacaataacc accgtgaata ggtctaaagt aaaacataag
    39841 aaatatttgg gggttagtct gctttttaat ccatattact ggatttttgt taagccgttt
    39901 aacggcgttc caggggcagg aaaaaaggat attcaggaga aaatgtgcgc tatatccgac
    39961 agttatgctg tgtaagctta ctctgcttaa gcggatctgc cgtcgccgcg aacgtccgtc
    40021 tacaggtcga ggggttatcg ggacagctgg aaaagaacgt tcgtgcgcag ctttctacga
    40081 ttgaaagtga tgaagtgacg ccagaccgtc gctttcgcgc acgcgtcgat gatgccatcc
    40141 gcgaaggtct gaaagcgctg ggttattacc agccgaccat tgaatttgat ctccgtccac
    40201 cgccaaagaa agggcggcag gtattgatcg ccaaagtcac gccaggtgtg ccggtgttaa
    40261 ttggcggcac cgatgtggta ttgcgcggcg gcgcgcggac cgataaagac tatttgaaat
    40321 tgctcgatac tcgcccggct attggtacgg tactgaacca gggcgattat gaaaatttca
    40381 aaaagtcctt aaccagtatt gcgttgcgta aaggttattt cgatagcgaa tttaccaaag
    40441 cgcagctggg cattgcgctc ggcctgcata aagccttctg ggatattgat tataacagtg
    40501 gcgaacgtta ccgctttggg catgtgacct ttgaaggatc acaaatccgc gatgaatacc
    40561 tgcaaaatct ggtgccgttt aaagagggcg atgagtacga atcgaaagat ctggcggaac
    40621 ttaaccgtcg actttctgct accggctggt ttaactcggt cgtagtggct ccacaatttg
    40681 ataaagcgcg cgaaacgaaa gtattaccct tgacgggcgt ggtttcgccg cgaactgaaa
    40741 acaccatcga aaccggggtc ggttactcta cggacgtggg accgcgcgtg aaagcgacgt
    40801 ggaaaaagcc gtggatgaac tcttatggtc acagtctgac caccagtacc agtatttccg
    40861 cgccggaaca gaccctcgac ttcagctata aaatgccgct gctgaagaat ccactggaac
    40921 aatattattt ggtgcagggc ggttttaagc gcactgacct gaacgatacc gaatctgact
    40981 ccactacgct ggtggcttct cgctactggg atctctccag cggctggcag cgtgccatta
    41041 acctgcgctg gagcctcgac cactttaccc agggtgaaat taccaacacc acgatgctgt
    41101 tttatcctgg ggtgatgatt agccgcacgc gttctcgtgg tggcctgatg ccaacctggg
    41161 gcgactcgca acgctactct atcgactact ccaacacggc ctggggctca gatgtcgatt
    41221 tctccgtttt ccaggcgcag aacgtctgga tccgcacact gtacgatcgc catcgttttg
    41281 tgacacgcgg cacgctgggc tggattgaaa cgggtgattt cgacaaagta ccgccggatc
    41341 tgcgtttctt cgccgggggc gatcgcagta ttcgcggcta caaatacaaa tctatcgccc
    41401 cgaaatacgc caacggtgac ctgaaagggg cctcgaagct gataaccgga tcgctggaat
    41461 accagtacaa cgtgaccgga aaatggtggg gcgcggtgtt tgtcgatagt ggcgaagcgg
    41521 taagcgatat tcgccgcagc gactttaaaa ccggtaccgg ggtcggcgta cgctgggaat
    41581 cgccggtcgg gccaatcaaa ctcgattttg ccgtaccggt cgcggataaa gacgagcacg
    41641 ggttacagtt ttacatcggt ctggggccag aattatgagt ttatggaaaa aaatcagcct
    41701 cggcgtggtt atcgttatct tactgttgct gggatcggtg gcgtttctgg tgggcaccac
    41761 cagcggcctg catctggtat ttaaagcggc ggatcgctgg gtgccaggac tggatattgg
    41821 caaggtcacc ggcggctggc gcgatctcac cttgtctgac gttcgttatg agcagccagg
    41881 cgtggcggta aaagcgggca atctgcatct ggcggtcggg cttgagtgcc tgtggaacag
    41941 tagcgtttgt attaatgacc tggcgctgaa agacattcag gtcaacatcg acagtaaaaa
    42001 aatgcctcct tctgaacagg ttgaagaaga ggaagatagc ggtccgctgg atctctccac
    42061 gccgtatccc atcaccctga cacgggtggc gctggacaac gtcaacatca agattgatga
    42121 caccacggtg tcggtgatgg acttcacctc cggcctgaac tggcaggaga aaaccctgac
    42181 cctgaaaccg acgtcgctga aaggcctgct gattgctctg ccgaaagtgg cggaagtggc
    42241 gcaggaagaa gtggtcgaac cgaaaattga aaatccgcag ccggaggaaa agccgctcgg
    42301 cgaaacgctg aaagatctct tttctcgccc ggtattgccg gaaatgaccg acttgcattt
    42361 gccgcttaac ctgaacattg aagagtttaa aggcgagcag ttacgcgtga cgggcgacac
    42421 ggacatcacc gtgcgcacca tgctgctgaa agtgagcagc attgacggca atactaaact
    42481 ggacgccctg gatatcgatt ccaaccaggg gatcgtcaac gccagcggca cggcgcagct
    42541 gtcagacaac tggccggtgg atatcaccct caacagcaca ctgaacgtgg agccgttgaa
    42601 aggtgaaaaa gtgaagctaa aagtgggcgg cgcgctgcgc gaacagctgg agattggcgt
    42661 taatctttcc ggtccggtgg atatggattt acgcgcccag acgcgactgg cggaagccgg
    42721 attgccgctc aacgtggaag tgaacagcaa acagctttac tggccgttca ctggtgagaa
    42781 gcagtatcag gcggatgatc tgaaactgaa acttaccggc aaaatgaccg attacacgct
    42841 ctctatgcgt acggcagtga agggacagga gatcccgcca gccaccatta cccttgatgc
    42901 caaaggtaat gaacagcagg tcaatctcga caaactcacc gtcgcggcgc tggaagggaa
    42961 aactgaactc aaggcgttgc tcgactggca gcaggccatt agttggcgcg gtgagctaac
    43021 gcttaatggc attaacaccg ccaaagagtt cccggagtgg ccgtcgaaac tcaatggctt
    43081 gattaaaacc cgcggtagcc tgtacggcgg cacctggcag atggaggtgc cagaactgaa
    43141 gctgaccggt aacgtcaaac agaacaaagt gaacgttgac ggcacgctga aaggcaacag
    43201 ttatatgcag tggatgatcc ctgggcttca tctggaactc gggccaaaca gtgccgaagt
    43261 gaaaggcgag ctgggggtaa aagatctcaa tcttgatgcc accatcaacg cgccggggct
    43321 ggataacgcg ctgccggggc ttggcggtac agcgaaaggg ctggtgaaag tacgcggcac
    43381 ggtggaagcg ccacaactac tggcggacat caccgcgcgc ggcctgcgct ggcaggaact
    43441 ttccgtggcg caggttcgcg tggaaggcga cattaaatcc accgatcaga tcgccgggaa
    43501 actcgacgta cgcgttgagc aaatttcgca gccggatgta aatatcaacc tcgtcaccct
    43561 gaatgccaaa ggcagcgaaa agcagcacga gctacagttg cggattcagg gcgagcctgt
    43621 ctccgggcag cttaatctgg caggaagttt tgatcgcaaa gaagaacgct ggaagggaat
    43681 tcttagcaat acccgcttcc agacgccggt cggcccgtgg tcgctgaccc gcgatattgc
    43741 tctggattac cgcaataagg agcaaaaaat cagcatcggg ccacactgtt ggcttaaccc
    43801 gaatgcggag ctgtgcgtgc cgcaaactat cgatgcgggg gccgaagggc gtgcggtggt
    43861 gaatctcaac cgcttcgacc tcgccatgct gaaaccgttt atgccgcaaa ccactcaggc
    43921 cagcggtatc ttcacgggta aagcggacgt tgcctgggac accacgaaag aggggctgcc
    43981 gcagggcagt atcacccttt cggggcgtaa cgtgcaggta acgcaaaccg tcaacgatgc
    44041 ggcgctgccc gtggcgtttc agacgctgaa tctgacggcg gaattgcgta acaaccgtgc
    44101 cgaattgggc tggaccatcc gcctgaccaa taacggccag tttgatggtc aggtgcaggt
    44161 gaccgatccg caaggccgcc gtaatcttgg tggcaacgtc aatatccgta acttcaacct
    44221 tgcgatgata aaccccatct ttacccgtgg ggaaaaagca gcggggatgg tgagcgccaa
    44281 cttgcgtctg ggtggtgatg tgcaaagccc gcagttgttt ggtcagcttc aggttacggg
    44341 tgtggatatc gacggcaact ttatgccgtt tgatatgcag ccgagccagc ttgcggtcaa
    44401 ctttaacggt atgcgctcga cgcttgccgg tacagtacgg acccagcagg gtgaaatcta
    44461 cctgaacggt gatgccgact ggagccaaat tgaaaactgg cgggcacgag taacggcgaa
    44521 aggcagtaaa gtgcggatca ccgtgccgcc gatggtacga atggatgtat cgccagatgt
    44581 tgtattcgag gctacaccaa acctgtttac cctcgatggt cgcgtggatg tcccgtgggc
    44641 gcgcatcgtg gtgcacgatc tgccggaaag cgcagtaggc gtctccagcg atgtggtgat
    44701 gcttaacgat aacctgcaac cggaagagcc gaaaacggcg tcgattccga ttaacagtaa
    44761 cctgattgtc cacgttggca acaatgtgcg cattgacgcc tttggcctga aagcgcggct
    44821 gacgggcgat cttaacgtcg ttcaggacaa acaagggctg ggcctgaacg ggcagatcaa
    44881 catccctcaa gggcgcttcc atgcctatgg tcaggatctg attgtgcgta agggtgagct
    44941 actgttctct ggtccgccag atcaaccgta tcttaatatt gaagctattc gtaacccgga
    45001 tgctacagaa gacgacgtaa tcgccggagt tcgcgtcact ggtctggcgg acgaaccgaa
    45061 agcggagatc ttctctgacc cggcgatgtc gcaacaagct gcattgtctt atttgctacg
    45121 tggacaaggg ctggagagcg atcagagcga cagtgcggcg atgacctcga tgctgattgg
    45181 cctgggggtt gcacaaagtg gtcagattgt gggtaaaatc ggcgagacgt ttggcgtaag
    45241 caatttagcg ctcgacaccg agggagttgg cgactcctcc caggtggtgg tcagcggcta
    45301 tgtattgcca ggtctgcaag tgaaatacgg cgtgggtata tttgactcta tagcaacact
    45361 cacgttacgt tatcgcctga tgcctaagct atatctggaa gccgtgtctg gtgtagacca
    45421 ggcactggat ttgctctatc agttcgagtt ttagcaatgc gaatatttgt ctacggcagt
    45481 ttacgccaca aacaaggcaa cagtcactgg atgaccaatg cccagttact gggcgatttc
    45541 agtatcgata actaccagtt gtatagcctg ggccactatc caggcgcagt tccggggaac
    45601 ggaacagtac acggtgaagt ttatcgtatt gacaacgcca cgctggccga acttgatgcc
    45661 ttgcgcacca ggggcggtga atacgcgcgc cagttgattc agacgccgta cgggagtgca
    45721 tggatgtacg tttatcaacg acccgtcgat ggattaaagc taattgaaag cggcgactgg
    45781 ttagacaggg ataagtaacc atatgcatac gccaccttcg ggtggcgttg ttttttgcga
    45841 gacgactcgc attctgtttt gtaattccct caccctttgc ttttctctcc gagccgcttt
    45901 ccatatctgt taacgcataa aaaactctgc tggcgttcac aaatgcgcag gggtaaaacg
    45961 tttcctgtag caccgtgagt tatactttgt ataacttaag gaggtgcaga tgcgtattac
    46021 cataaaaaga tgggggaaca gttcaggtat ggtcattcct aatgttgtga tgaaagaact
    46081 taacttacgg ccggggcaga gcgtggaggc gcaagtgagc aacaatcaac tgattctgac
    46141 acccatctcc aggcgctact cgcttgatga actgctggca cagtgtgaca tgaacgccgc
    46201 ggaacttagc gagcaggatg tctggggtaa atccacccct gcgggtgacg aaatatggta
    46261 aagaaaagtg aatttgaacg gggagacatt gtgctggttg gctttgatcc agcaagcggc
    46321 catgaacagc aaggtgctgg tcgacctgcg cttgtgctct ccgttcaagc ctttaatcaa
    46381 ctgggaatga cgctggtggc ccccattacg cagggcggaa attttgcccg ttatgccgga
    46441 tttagcgttc ctttacattg cgaagaaggc gatgtgcacg gcgtggtgct tgtgaatcag
    46501 gtgcggatga tggatctacg cgcccggctg gcaaagcgta ttggtctggc tgcggatgag
    46561 gtggtggaag aggcgttatt acgcttgcag gcggtggtgg aataaggtgt gtttatttat
    46621 cgcgggcata aaaaaaccct tactaaccga agcccggtgt tcagggttat tgcgccagaa
    46681 gaacttattt attctttgcg cgctcgaagg aggcaacgat ttcagcttta gcggcttctg
    46741 cgttttccca accttcaact ttcacccact tgcctttttc gaggtctttg tagtgctcga
    46801 agaagtgagc gatctgcgct ttcagcagct ctggcagatc gttaacgtct ttaatgtgat
    46861 cgtattcttt gctcagcttg gtgtgcggaa cagcaatcag tttcgcatct tcaccggctt
    46921 cgtcggtcat tttcagaacg ccaaccggac ggcaacggat cacagaaccc ggctgcagcg
    46981 ggtacggagt cgggaccagt acgtcaaccg ggtcaccgtc cagagacagg gtgtggttga
    47041 tgtaaccgta gttgcacgga tagaacatcg cggtggacat gaagcggtca acgaacagtg
    47101 cgccgctctc tttgtcgatt tcgtatttga tcggatctgc gttagccggg atctcaataa
    47161 ccacgtagat gtcttccggc agatctttac ccgcagggac gttgagtaag ctcatgtctg
    47221 tttcctttaa aaatatgtgg caaacaagtg ccgagtatta tagccaactc gcgccgaatg
    47281 tcttcgcttg ttttcgtctt cattttcctt tttcgccagt tttaagacgg tatgcagagc
    47341 aggaaaatcc ataaccgtag ccgcattttt catagtgaga tgaaagcgat tacaaacttg
    47401 tgattaacgt tttatttact tttttgaagt gtgatgtaac gcaatccgtt acataacgaa
    47461 ttgtctatag ttttttcgcg aacatctttt caccaataat aattaccccg acgaggacaa
    47521 ccctatgtgg aaacgcttac ttgtagtctc tgcagtctcg gcagccatgt cgtctatggc
    47581 gttggccgct ccattaaccg taggattttc gcaggtcgga tcggaatccg gctggcgtgc
    47641 cgcagaaacc aatgtggcga aaagtgaggc cgaaaaacgc ggaattacgt tgaaaattgc
    47701 cgatggtcag caaaagcagg aaaaccagat taaagcggta cgttccttcg ttgcacaagg
    47761 ggtggatgcg atctttatcg ctccagtggt tgcgacgggt tgggaaccgg tattaaaaga
    47821 ggcgaaagat gccgaaatcc cggtcttctt gctcgaccgt tccatcgatg tgaaagacaa
    47881 atctctctat atgaccaccg ttactgccga caacatcctc gaaggtaagt tgatcggtga
    47941 ctggctggta aaagaagtga atggcaaacc atgcaacgtg gtggagctgc agggcaccgt
    48001 tggggccagc gtcgccattg accgcaagaa aggctttgcc gaagccatta agaatgcgcc
    48061 aaatatcaaa attatccgct cgcagtcagg tgactttacc cgcagtaaag gcaaagaagt
    48121 catggaaagc tttatcaaag cggaaaacaa cggcaaaaac atctgcatgg tttacgccca
    48181 taacgatgac atggtgattg gcgcaattca ggcaattaaa gaagcgggcc tgaaaccggg
    48241 caaagatatc ctcacgggtt ccattgacgg tgtaccggac atctacaaag cgatgattga
    48301 tggcgaagcg aacgccagtg ttgaactgac gccgaatatg gcgggacccg cttttgacgc
    48361 gctggagaaa tacaaaaaag acggcaccat gcctgaaaag ctgacgttaa ccaaatccac
    48421 cctttacctg cctgataccg caaaagaaga gttagagaag aagaaaaata tggggtattg
    48481 agggttgcta tgcctgatgc cgattcgtag gccggataag gcgctcgcgc cgcatccggc
    48541 ggtggtgcgc tgaagcctga tgcgacgctt accgcgtctt atcatgccta ctgggagcac
    48601 gctttacacc gggggaaacc atgacgacta accaacacca ggagatcctc cgcaccgaag
    48661 gattaagtaa atttttcccc ggcgtcaaag cgttagacaa cgttgatttc agcctgcgcc
    48721 gtggcgaaat catggcgctg ctcggtgaaa acggggcggg aaaatcgacg ctaatcaaag
    48781 cattaaccgg tgtataccac gccgatcgcg gcaccatctg gctggaaggc caggctatct
    48841 caccgaaaaa taccgcccat gcacaacaac tcggcatcgg aaccgtttat caggaagtca
    48901 acctgctacc caatatgtcg gtcgctgata atctattcat aggtcgcgaa cccaaacgtt
    48961 tcggctttct acgccgcaaa gagatggaaa agcgtgccac cgaactgatg acatcttacg
    49021 gtttctccct cgacgtgcgt gaaccgctta accgtttttc ggtggcgatg cagcaaatcg
    49081 tcgccatttg ccgggctatc gatctctccg ccaaagtgct gatcctcgat gaacccaccg
    49141 ccagcctcga cacccaggaa gtggagctat tatttggcct gatgcgtcag ttgcgcgatc
    49201 gcggcgtcag cctgatcttt gtcactcact ttctcgatca ggtctatcag gtcagcgatc
    49261 ggatcactgt cttacgcaac ggcagtttcg taggctgtcg ggaaacgcgc gagctaccgc
    49321 agatcgaact ggtaaaaatg atgctggggc gcgagctgga tacccacgcg ctacagcgtg
    49381 ccgggcgaac attgttgagc gacaaacccg ttgccgcgtt caaaaattac ggcaaaaaag
    49441 gaacgatagc accgtttgat ctcgaagtgc gccccggcga gatcgtcggt ctggctggct
    49501 tgctgggatc cggacgtacc gaaaccgccg aagtgatctt cggtatcaaa cctgctgaca
    49561 gcggcacggc gttgatcaaa ggcaaaccgc aaaccctgcg atcgccacat caggcttcag
    49621 tactgggcat cggattctgc ccggaagaca ggaaaaccga tggcatcatc gccgccgcct
    49681 cggtgcggga aaatatcatc ctcgctctcc aggcccagcg cggctggcta cgacccattt
    49741 cccgcaaaga gcagcaagag attgccgaac gctttatccg ccagcttggc attcgcacac
    49801 cttcaactga acaaccgatt gaatttctct ccggtggcaa tcagcaaaaa gtgctgcttt
    49861 cacgttggct actgacccga ccgcaatttc tgatccttga tgagccaacc cgtggcattg
    49921 atgttggtgc ccacgccgag atcatccgcc tgattgaaac gctatgcgcc gatggtctgg
    49981 cgctgctggt gatctcctcc gaactggaag aactggtggg ctatgccgac cgggtgatca
    50041 tcatgcgcga tcgcaaacag gtggcggaga tcccgctggc agagctttcc gttccggcga
    50101 tcatgaacgc cattgcggcg taaggagaac agtgtgatgc ctcaatctct tccggacacc
    50161 actacgccaa aaaggcgctt tcgctggccg acggggatgc cgcagctggt agcactattg
    50221 ctggtgctgc tggtcgacag cctggtagcc ccgcatttct ggcaggtagt gcttcaggat
    50281 gggcgtttgt tcggtagccc catagacatt cttaaccgtg cagcccccgt tgcgttactg
    50341 gcgattggta tgacgctggt gatcgccacc ggtgggattg atctctccgt aggggcggtg
    50401 atggctatcg ccggagccac aacggctgcg atgacggtcg cgggagtcag cctgccgatt
    50461 gttttgttaa gcgccctggg caccggcatc ctggcgggat tgtggaacgg catactggta
    50521 gcgatcctca aaattcagcc atttgtcgcc accctaatcc tgatggtcgc cgggcgcggc
    50581 gtggcgcaac tgatcacctc cggccagatc gtcacgttta actcgccgga tctctcatgg
    50641 tttggcagcg gatcgctgtt gttcctgcca acgccggtca ttatcgcggt gctgacgctt
    50701 ctcctgttct ggctgttgac ccgcaaaacg gcgctgggga tgtttatcga agccgttggt
    50761 atcaacattc gggcggcaaa aaatgccggg gtaaacacgc gaatcatcgt catgctcacc
    50821 tacgtgttga gcgggctatg tgcggcgatt gcgggcatta tcgtggcggc ggatattcgc
    50881 ggtgccgatg ccaacaacgc cggattatgg ctggagctgg acgccattct cgcggtggtg
    50941 attggcggcg gatcgctgat gggcgggcgc tttaacctgc tgctctcggt agtgggggcg
    51001 ctgattattc aggggatgaa caccggaatt ttgctttcgg gctttccacc ggagatgaac
    51061 caggtggtga aagcggtggt ggtgctttgc gtgctgattg tccagtcgca acgctttatc
    51121 agtctgatta aaggagtacg tagccgtgat aaaacgtaat ttgccactga tgatcaccat
    51181 cggcgtcttt gtgctgggct atttctactg cctgacgcag tttcccggtt ttgcttccac
    51241 aagagtgatc tgcaatatcc tgaccgataa cgcttttctg gggattattg ccgttggtat
    51301 gacctttgtg atcctctctg gcggtatcga tctctccgtc ggttcggtga tcgcttttac
    51361 cggcgtgttt ctggcaaaag tgattggcga cttcggcctc tcgccgttgc tggcatttcc
    51421 gctggtgctg gtaatgggct gtgcctttgg cgcatttatg gggcttctga tcgacgccct
    51481 gaagatcccg gcatttatca ttacgcttgc ggggatgttc tttttgcgcg gcgtcagcta
    51541 tctcgtttcg gaagagtcga ttccgataaa ccatcccatt tatgacacgc tctcaagcct
    51601 tgcgtggaaa atccctggcg gcggtcgctt aagtgcgatg gggctgctga tgctggcggt
    51661 ggtggttatc ggcatattcc tcgcgcatcg tacccgtttt ggtaatcagg tatacgccat
    51721 tggcggcaac gcaacgtcgg caaacctgat ggggatttct acccgcagca ccactattcg
    51781 catttatatg ctctccaccg gactggcaac gctggcgggg attgtcttct cgatttatac
    51841 ccaggccgga tatgcgctgg cgggcgtagg tgtggaactg gacgctatcg cctcagtggt
    51901 gattggcggt acgcttttga gcggtggcgt tggaacggta ttagggacgc tttttggcgt
    51961 ggcgattcag ggactgattc agacttacat aaactttgat ggcacgctga gttcctggtg
    52021 gacgaaaatc gccatcggta ttttgttgtt tatttttata gcattacagc gtggattaac
    52081 ggtgctgtgg gagaatcgtc agagttcgcc agtgacaaga gtcaacattg cgcagcaata
    52141 aaaacgcctc tccgtgtgga gaggcgcagg agattacgcg tccgggaact cacggataaa
    52201 gcgttcgaca tcttcaacca tatggtcgtt gccgacaaag aacgaacggc gctggtgcag
    52261 ggtttccggg ataatatcca gaatacgctc tttgccatcg ctcgctttac cgcccgcttg
    52321 ttccgccagg aatgccatcg ggttgcactc atacagtaaa cgcagtttac cgtccgggtg
    52381 gctggcggtg cttgggtaga gataaatacc gcctttcagc aggttacggt ggaaatccgc
    52441 aaccagtgaa ccgatataac gtgaggtata cgggcggttg gtggatttat cttcttcctg
    52501 gcagaattta atgtacttct tcaccccgtt cggaaactta atgtagtttc cttcgttgat
    52561 ggagtaggtt ttgcctttct ccgggaagcg catccgttcc tggcacaggc agaaaacgcc
    52621 cagcgaagga tcgtaagtaa aggcgtgaac accgcatccg gtggtgtaaa ccagcatagt
    52681 agaggagccg tataccacgt aacctgccgc aacctgtttg ttaccaggct ggaggaaatc
    52741 ttcttccgtt accggcgtgc caacaggcgt aacgcggcgg tagatggaga aaatggtacc
    52801 gacagagacg ttaacatcga tgttggacga gccatccagg gggtccatca gcaccacgta
    52861 ttttgcgtgt tcacagcctt caaagacgac aatctcatct tcttcttcag aggcaatgcc
    52921 cgcaacgata tcgcgtgctt tcagtgcggc tttcagtttt tcattagcga acaagtcgag
    52981 tttctgctga acctcgccct gcacgttctc agcaccgctg gcacccagga tatcaaccag
    53041 tcctgctttg ttgatatcgc gatggataat cttggcgccc agttttattg ccgacagcaa
    53101 agcagtgagc tcaccggtag catgagaaaa ctcgtgctgc ttttcgacaa taaattcacc
    53161 taacgttttc ataaaacttt ccctgcaatg tttatggagt aaagcgaccg caacaatctt
    53221 aacaaataat ctcaatgttg cgctcaggtg aatcgcgcca gcaaattgcg gattatcctg
    53281 aaatgcgttt ctcacttgcc cgacatatgc gtaaaatgag cggcagatta aaaaaggata
    53341 gtgacgtatg cgcattcata ttttaggaat ttgtggcacg tttatgggcg gtctggcgat
    53401 gctggcgcgc cagttaggcc atgaagtaac gggttcggac gccaatgtgt atccgccgat
    53461 gagcacctta cttgagaagc aaggcattga gctgattcag ggttacgatg ccagccagct
    53521 cgatccgcag ccggatctgg tgattattgg caacgccatg acccgtggaa atccgtgtgt
    53581 ggaagcggta ctggaaaaaa acatccctta tatgtcaggt ccacagtggc tgcacgattt
    53641 tgtgctgcgc gaccgctggg tgctggccgt tgcaggtaca cacggcaaaa ccaccaccgc
    53701 gggaatggcg acctggattc tggaacagtg tggttacaaa ccgggctttg taatcggcgg
    53761 tgtgccgggg aactttgagg tttcggcgcg tctgggcgaa agcgacttct ttgttatcga
    53821 agcggatgag tacgactgcg ccttcttcga caaacgctct aaatttgtcc attactgccc
    53881 gcgtacgctg atcctcaaca accttgagtt cgatcacgcc gatatctttg acgacctgaa
    53941 agcgatccag aaacagttcc accatctggt gcgtatcgtt ccggggcagg gccgtattat
    54001 ctggccggaa aacgacatca acctgaaaca gaccatggcg atgggctgct ggagcgagca
    54061 ggagctggtg ggcgagcaag gtcactggca ggcgaaaaag ctgaccactg acgcttctga
    54121 atgggaagtc ttgctggatg gcgaaaaagt gggcgaagtg aaatggtcgc tggtgggcga
    54181 acataatatg cacaatggcc tgatggcgat tgcggcggct cgccatgttg gtgtagcgcc
    54241 tgcagatgcc gctaacgcgc tgggttcgtt tattaacgct cgtcgccgtc tggagttgcg
    54301 tggtgaagcg aatggcgtga cggtatatga cgattttgcc catcacccga cggcaattct
    54361 ggcaacgctt gcggcgctgc gtggcaaagt tggtggtacg gcgcgcatta ttgctgtgct
    54421 ggagccgcgc tcgaatacca tgaaaatggg gatctgcaaa gacgatctgg caccttcatt
    54481 aggtcgtgcc gatgaagtct tcctgctgca accggcgcat attccgtggc aggtggcaga
    54541 agtggcagaa gcctgcgttc agcctgcaca ctggagtggc gatgtggata cgctggcaga
    54601 tatggtggtg aaaaccgctc agcctggcga ccatattctg gtcatgagca acggcggttt
    54661 tggtgggatc catcagaaac tgctggatgg tctggcgaag aaggcggaag ctgcgcagta
    54721 attcggcctc agcctgagat agcattgccg gataaggcgt ttacgccgca tccggcaaac
    54781 cattctcaca tcaacaacga atgttaccct tcgttttccg ctaactcgcg cagatactgg
    54841 aaaatctggc gtgcggattt cggcggctta ttcccttctt tctctttctt cgcattgcgg
    54901 atcagagtac gcagctgctg acgatcggca tcgggccaca gattcaatac ttcagcgaga
    54961 gcgtcatctc cctggtcgat cagacgatcg cgcaggtttt ccagtttatg gaacagcacc
    55021 acctgctggt tgtgacggtt tttcagctta tccagcgcct gacggatcgg ttcaacatcg
    55081 cgctggcgca gcatcttacc aatgagctgc aactggcggc ggcgaccttc catcttaata
    55141 cgctgggcga gctcaatagc cgcgcgcaga tccgcatcta acgggatctt atccagcgcg
    55201 tttttcccca gatcaacaat ttccgcgcca aggcgtttta gctcctcggc atcgcgttta
    55261 atttcacttt tactgaccca gataatttca tcgtcttcgt cttcgatgtc atcaccggga
    55321 acgtcgtcga gccagtcttc gggctgctta gtcatctcag gctccttaaa aaaagaggct
    55381 aatgttacca gttaagatgc gcactgaaaa acggttctct gttagacttc agagaaactc
    55441 tctacattat ggcacttgca atgaaagtaa tctctcaagt tgaagcgcag cgcaagattc
    55501 tggaagaagc agtttccact gcgctggagt tggcctcagg caaatcggac ggtgcggaag
    55561 ttgccgtcag caagaccacc ggcattagcg taagcacgcg ttatggtgaa gtggagaatg
    55621 ttgaattcaa tagcgatggc gcgctgggga tcactgttta tcaccagaac cgcaaaggta
    55681 gcgcatcatc caccgattta agcccgcagg ccattgcccg tactgtacag gcggcgctgg
    55741 atattgcccg ttatacctcg ccagatccct gtgccggcgt ggcagacaaa gagctgctgg
    55801 cctttgacgc accagatctc gacttgttcc accctgcgga tgtttccccg gatgaagcca
    55861 ttgaactggc ggcccgcgca gaacaggcgg cattgcaggc ggacaaacgc atcaccaata
    55921 ccgaaggtgg cagctttaac agccactacg gtgtcaaagt ttttggcaac agccacggca
    55981 tgttgcaggg ttactgctca acgcgtcatt cgctctccag ctgtgtaatt gccgaagaaa
    56041 atggcgatat ggagcgtgat tacgcctaca ccattggccg cgcgatgagc gatctgcaaa
    56101 cgccagagtg ggtcggggcc gactgtgctc gccgtacttt atcgcgcctg tcaccgcgta
    56161 aactctccac catgaaagcg ccggtcattt ttgccaatga agtggcaacc gggctttttg
    56221 gccatctggt gggggcgata gcgggtggat cggtttaccg taaatctacc ttcctgctgg
    56281 attcgctggg taaacaaatt ctgccggact ggctgaccat tgaagagcat ccgcatctgc
    56341 tgaaagggct ggcgtcgacg ccattcgaca gcgaaggtgt gcgcaccgag cgtcgcgata
    56401 ttattaaaga tggcatcctg actcagtggc tgctgaccag ctattcggcg cggaaactgg
    56461 ggctgaaaag caccggacat gcgggcggta ttcacaactg gcggattgcc ggacaagggc
    56521 taagcttcga acaaatgctc aaagagatgg gcaccgggct ggtggtgacg gagctgatgg
    56581 gccagggcgt gagtgccatt actggtgatt attcccgtgg tgctgcgggc ttctgggtag
    56641 agaatggcga aattcagtat ccggttagtg aaatcaccat cgcaggtaat ttaaaagata
    56701 tgtggcgcaa tattgtcacc gtcggtaacg atattgaaac acgcagtaat atacagtgtg
    56761 gttctgtgct gttgccggag atgaaaatcg ccgggcagta aaaaactggc gcgacctgcc
    56821 gcgctataaa ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat
    56881 gagagccggg gagcgtactc gcagtacgtg acccgggcga atgagcgtag ccgtcgatga
    56941 ggcagtgtga aggatgaagt gtaaataaaa aaggaagtga gcaatgcgta aaagcctgtt
    57001 agctattctt gcagtctcct cgttggtatt cagttcggcg tcatttgctg ctgatctgga
    57061 agacaatatg gaaatcctca acgacaattt aaaagtggtc gaaaaagcgg ataacgcgtc
    57121 gcaagtcaaa gacgcgttaa ctaaaatgcg cgctgcggcg ctggatgcgc aaaaagcaac
    57181 gccgccgaag ctggaaggca aatcaccgga cagcccggag atgaaagact tccgccatgg
    57241 tttcgatatt ctggtcggtc aaattgacga cgcgctgaag ctggcaaatg aaggtaaagt
    57301 aaaagaagcg caggccgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa
    57361 gtatcgttaa ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg
    57421 caaatgatgc accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc
    57481 ctgcacaaac ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgcagc
    57541 gttgagttcg tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc
    57601 gcggatgcgt tttaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc
    57661 gccggagagg gaaatcccct ggcgtttgat acgagtgtca ttcagatctt taatgatctt
    57721 gtcttccatt gctttggtaa atggctgacc agaatttacc cgccatgtgc ttttgttata
    57781 gcaaccgggg cattcatgaa cacacccgga gacaaacagg gtgcagcgag tgccggggcc
    57841 gttgacgatg tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt
    57901 ccgcgtgtag gctgcatccg gtaatcaatg cctgatgcga cgctgtcgcg tcttatcagg
    57961 cctacagccg ttgccaaatg taggccggat aaggcgttta cgccgcatcc ggcatttgct
    58021 tagcgcagag tgaagattaa cctatctgcc cattccccaa atgtttaacg cggcgcttaa
    58081 cttcttcctg cttaccagcg ttaaacggac gtgcatccgg gctacctaaa tatccgcaca
    58141 cgcggcgagt taccgacaca cgggaggcgt catggttacc acatttcggg caagtgaagc
    58201 ctttgctggt gcactcgaac tcaccggtaa agccacactc gtagcactca tcaatcggtg
    58261 tattggtgcc gtaatacggt acatgctgat agctgtaatc ccagacatct tccagcgctt
    58321 tcaggttgtg ctgaatgttt ggatactcgc cgtagcaaat gaaaccaccg ttcgccagcg
    58381 gcgggtaagg cgcttcaaag tcgatcttgt cgtacgggtt caccttcttc tccacatcaa
    58441 ggtggaaact gttggtgtag taacctttat cggtcacgcc cggcaccacg ccaaactcag
    58501 cagtatcgag acggcagaag cggtcgcaca ggttttcact cggcgtgctg tagagactga
    58561 aaccataacc cgtttcttct ttccactgat ccactgcctg acgcagacgt tcaacaatgg
    58621 cgataccttt cgcgcgaagc tgctcgttgt cgtaaacatg ctcgccgccg aacagcgcgt
    58681 taatggtttc gtggatgccg atgtaaccca gcgaaataga cgcacgaccg tttttgaaga
    58741 tttcagaaac atcatcgtca gcattcagac gtacgccaca agcaccttcc atatagagga
    58801 tcggggcgac gcgcgctttc acgccttcga gacgagcgat acgggtcatc agtgccttac
    58861 gtgccagcac cagacgttca tccagcagct tccagaaggt ggcttcatcg ccttttgctt
    58921 ccagagcaat acgcggcagg ttcaggctga tcacgccgag gttgttacga ccatcgtgga
    58981 tctgctcgcc gttttcattt tcccacacgc cgaggaagct gcggcagccc atcggggttt
    59041 taaacgaacc ggtgactttc actacctgat cgtagttcag gatatccgga tacatgcgct
    59101 tgcttgcgca ctccagcgcc agctgtttga tgtcgtagtt cggatcgcct tttttatggt
    59161 tcagaccatc gcgaatcgca aacaccagtt tcgggaacac cgcagtttta cggtttttac
    59221 cgaggcctgc gatacggtta cgcaggatcg attcctgaat caggcgcgat tcccagctgg
    59281 tgcccagacc aaaaccaaag gttacaaacg gcgtctgacc gttggcggtg tgcagggtgt
    59341 ttacttcgta ctccagcgac tggaaggcat cgtagcactc tttgatggta cgagagttag
    59401 cgtagccttc ggcgtccggg atgttccact cttctgcggt cttgcgatgt ttgttgtagc
    59461 tggcggtaac aaacggcgcc agcacttcat cgatacggtt aatggtggtg ccgccataaa
    59521 tatggctggc gacctgagca ataatctgcg cagttaccgc ggttgccgta gagatcgact
    59581 tcggcggttc aatctcggcg ttccccattt taaagccctg ggtcagcatg cctttcaggt
    59641 cgatcaacat gcagttgaac atcgggaaga acggcgagta atcgagatcg tgatagtgaa
    59701 tatcgccacg ctcatgtgcc tgcaccacgt cacgcggcag caggtgctgg cgtgcatagt
    59761 gtttagccac gatcccggcc agcaggtcgc gctgggttgg aatcaccttg ctgtctttgt
    59821 tggcgttttc gttgagtaac gaggcgttgg tctgctcgac cagaccacgg atctcctggt
    59881 tcaggcgacc gcgtttttca cgttcaatgt cgcgatcgtg acggtactcg atgtaagcac
    59941 gagccagttg tttgtatgga cccgacatca gctggttttc aactgcggtc tggatctcgt
    60001 tgatatccac ctggttgcgg ccctgcatct gctcgctgac aaccgcggca acagtggcgc
    60061 aataatcggc atcatcgact tccgctgctt tagctgcacg cagaatcgct tctttgatgc
    60121 gctctgattt aaacggcact ttgcagccgt ctcgtttcat cacatgcggt gtcatgatcg
    60181 ctccatattt ttaagaacag gttatccaca gaaattggga aaggcgtttt ccggtttttt
    60241 cgtttcgttt tccggtgctt tccgcaatcg ccatccgctt atccacaacg ccgcaccttt
    60301 ttcgtgggga gctgtagtag caattatagt tgattaatac aacatattgg gttgggacgc
    60361 attttaaagt ctatatatag tgctttgcat caaggatgtt tgaacttttt ttgatgtagc
    60421 tcaaagtaaa aagcagagcg tacggatgac gggcgctaca gcgttatgta aattttttaa
    60481 tgaatttgct ggttgaaaaa tcaacaaaaa caacatgctg acagacaaaa ccccggaatg
    60541 acaggcattc ccggggctta gcggatactt acttctgtaa ccaccagaca gcctcaaaag
    60601 gccgtaaagt catggcacag ggttgtggtg aggcttcttc gtagttatgc atcacaagct
    60661 gccagttgcc gcgcatttgc cctggctgcc agggttggat ctcacggcta aggttggcaa
    60721 tgaccagcaa ggtttgcccc ttccattcac ggcgatagca ccacaataca gggctgtttg
    60781 gcagcagatc ctggtaattg ccccatgtca ggatggcttc ttgcttacgc agtgcgatta
    60841 acttttggta ggtgtaaaac accgaggagt catcggccag cgcggcttct acgttgattt
    60901 gttgatagtt atcgcccagg ccaatccacg gttcgccagc cgtaaacccg gcattatcgc
    60961 cgttgctcca ttgcatgggc gtgcggctgt tgtcacggga tttactggcg aggattgcca
    61021 ataactcgtc ggcatcacga ccatcgttgc gcagctcggc aaacatattg aggctctcca
    61081 cgtcgcgata gtcagtaatg cgcgtgaaat gcgggttggt catgccaatc tcttcgccct
    61141 ggtagatata cggcgttccc tgcatgccat gcagcaccat cgccagcatt tttgccgcag
    61201 gcacgcggta ttcaccttca tcaccaaagc gagaaacaat gcgcggctga tcgtggttac
    61261 accagaacaa ggcattccat gccacgttgt gcattccttg ttgccagtgg cggaacaatg
    61321 ttttcaacgc cacaaagtca ggtttagcca gcgtccattt ttcaccaccg ggataatcga
    61381 ccttcaggtg atgaaaatta aaggtcatcg acaattcact gcctgtcagt gccgcgtatc
    61441 gctggcaatg ctcaaggctg gtggaggaca tttcacctac ggtcattaac ccgcgtggcg
    61501 taaacacatc gcggttcatc tcgtgcaaaa actcgtgtgc tcgtggcccg tcggtgtaga
    61561 agcgacgccc gtcgccatcc aggtcttcag ggaaacgcgg gtctttggag atcagattca
    61621 ccacatccag gcgcaacccg tcgaccccac gatcggccca gaattcacag acttttttca
    61681 gctctgcgcg taccgctgga ttctcccagt tgagatccgc ctgttctggt gcaaagagat
    61741 gcaaatagta ctgttcgctt tccgcatgcc agcgccacgc actaccgcca aattttgaac
    61801 gccagttgtt cggtggcgtt tctggttcgc catcgcgcca gatataaaac tggcggtaag
    61861 ggctttcttt gttcagcgcc tcgcgaaacc aggcatgttg ggtagaggta tggttaaaca
    61921 ccatatcgag aatgatacga atcccgcgcg attttgcctg cgtcaccagt tcgtcaaaat
    61981 cgtccagcgt gccgtagatg ggatcaatcg ccgtatagtt cgctacgtcg taaccgttat
    62041 cgacctgggg agagatataa aagggggtta gccagatggc atcaacgccc agtttatgca
    62101 gatagtccag gcgttggata acgccacgta aatcgccggt gccgctaccc gtggtgtcct
    62161 gaaaactctt tggataaatc tggtagataa cgccgttttg ccaccagtgg ggaagattag
    62221 tcattacgtt attcctgcaa atgcgagggg gcgcaattgc gccccgaaga aaattaaaca
    62281 atatccagcg tgcccaggcg gtatttccgc tgatagataa acgaggtgag tacaatcggg
    62341 atgatgatgg cgataaccat tgccagcgca aatacctgcc agtagctcgg ttgaatcgag
    62401 agaattcccg gcaggccgcc tacgccgata ccattcgcca taacgccgtt caggccgcat
    62461 agtaatcctg ccagaccaga accaatcatc gcgcacagca tcgggaagcg atatttcagg
    62521 ttgatgccgt acattgcagg ctcagtgacc ccaagccagg cggagatagc ggcaggcacg
    62581 gagatctcgc gttcattgtg cttgcggctg gaaatgataa tgcctatcac ggcggagccc
    62641 tgagcgatat tcgacagcgc aatcagcggc cacactggcg tgccacccat gctttgaatc
    62701 atctgcaaat caatagcaag ggtggtctgg tgtacgccgg tgatcaccag cggcgcgtac
    62761 aggaagccaa acaatgccgc accaatcgga gcaaagctgc cggtcatcag gtgacgtacc
    62821 gcaaaggcaa cgccatcgcc aatcatgcga ccaaacggac caatcagcgc atgggcgagg
    62881 aacaccgcga ggatcagcga acagacgggc accaccacca gatagaggta atccggtacg
    62941 atgcgtttaa ggcgagtttc aataacgccc agtgccagcc cggctaacag tgccgggatc
    63001 acctgtgcct gatagcccac tttggcgatg ctgaacatgc caaagtccca cacttccggc
    63061 agctgctgcc cgagcagata agcgttcatc agctggggag aaaccagtgt cacgccaagc
    63121 acgataccaa ggatcggcgt gccgcccatt tttttcaccg ctgaccagca aataccgacc
    63181 ggcaggtaga agaagatcgc ttcaccgatc aaccacagaa aatcgtagat cgtttgcagg
    63241 gaagggtaca tttgcgccag cgtctgaccg ttgctcatgg gcaaatcgcc gatcacattg
    63301 cgaaaaccga ggatcaaacc gccgctaatc aacgcgggca gcaacgggaa gaagatctcc
    63361 gcgaaatgag agatcaactg ctcatgccat ttcatattct gccgggcggc tttttttacc
    63421 tgctctttat caacctgcgc ctgtccggtt gacgctatta gtgcttgata gtaatcaccc
    63481 acgttggtgc caatcaccac ctgaaattgc ccggcattgg tgaaacagcc tttcaccatg
    63541 gggagttgct caatttcttt cggtctggca ttggccggtt ggttgaggac aaagcgcagg
    63601 cgagtaatac agtggctcac cgtcgcaata ttgccgcgcc cgccgaccag ttcaatcaac
    63661 cgatcgatat ccgtttggtt tattttgctc atcataaagc cccatggcag atgacatttt
    63721 tggttggctg cagaatatat tgcgcggaaa aatttaaaaa cgggaacgtt cccgaaacgc
    63781 agcgaagatc acaatttatc gttcaggaaa cgatcaggac agggtggcgg ggatgatgat
    63841 ttgttgcggt tcgctgcgcc cggttacctg tgcgatcaat tggcaggccg cctggcgtcc
    63901 agcttcggcg taaccaggat ctacggtgac gatctccgga tgaaggaatt tcattaatgg
    63961 cgtattaccg acgctcgcca gttgcaaggt gtcgatgcgt tgctcttgca ggtatttact
    64021 tgcgccaagt gccagcgtgt cggttgcgca cagtaaggcg gtattttcag gcgtaatcac
    64081 ttttgctacg ttctcatagc cttgcttcat agcaaggcct ggcagagcgg caacgggatg
    64141 cagtttatgc gctttgcaga acgccaggta ggcttcgtga cgtcgcttcc cggtcgtcac
    64201 gtcactatgc ggcacgccga gataactgat attacgatgc ccctggtcat acagccgttg
    64261 catcaggatc ttgattgccc cttcgtcgtc ataacagacc gaagcaaaac cttttgcgtc
    64321 acgcgccagc agaaccagcg atgactgcca gtgggctaac atttcttctg ttatgccagt
    64381 aaaaccgaac agcactacgc cgtcgatatt acgccgtttc agcaccccca aatgttcggc
    64441 aactaattgc ggggaaaact gactttccat catgattggg tcgtaacctt gttcatagaa
    64501 cgctggcagc atggtttgaa cggcgagatt ttctgacaac gaatccaggc gggtaacaat
    64561 gatggcgacc actttatcgc tttgcccacg catagcgcgc gcagagcggg aaggggaaaa
    64621 tccatgctga ttcatcactg cttcaacacg ctcgcgggtg cgctggctca cgccgctttc
    64681 gttattcagc acccgggaaa ctgtagattt ccccacgccg cttaagcgtg cgatgtcttt
    64741 gatggtcagc cgattttgca tcctgttgtc ctgtaacgtg ttgtttaatt atttgagcct
    64801 aactttaccc gtgcattcag caatgggtaa agtctggttt atcgttggtt tagttgccag
    64861 caggtattat atcgccatag atgctacgaa tattattgga ttctccttat tatttgcggc
    64921 gctttattca cttaccggag gttatatgga acctgatccc acgcctctcc ctcgacggag
    64981 attaaaactt ttccggtaag cccgtctttt cacggcgtta ccggatgcgt aaggccgtga
    65041 cgttttaacg tccctgctca gctttattac cttcaggtaa ggcttcgcca cgcctgaaga
    65101 catttctgta ctgtttcaga cagtgcggag ggactcctta tgtttaaaga aatctttacc
    65161 cggctcattc gccatttacc ttcccgtctg gttcatcgtg atccattgcc tggcgcgcag
    65221 cagacagtga atacggtggt cccgccgtcc ttaagtgcgc attgcctgaa aatggcggtg
    65281 atgcccgaag aagaattgtg gaaaacgttc gacacccatc cggaagggtt aaatcaggcg
    65341 gaagtggaat ctgcccgcga acaacatggt gaaaataaat tacccgcaca acaaccgtcg
    65401 ccgtggtggg tacatttatg ggtctgctat cgcaacccat ttaatatttt actcaccatt
    65461 ctcggcgcta tttcttacgc cacggaagat ttatttgccg cgggcgttat cgcgctaatg
    65521 gtcgctattt ctacgttgct gaactttatt caggaagcac gttccactaa agcggcagat
    65581 gccctgaaag cgatggtcag caataccgcg acggtgctgc gcgtaattaa cgacaaaggc
    65641 gaaaatggct ggctggagat cccgatcgac cagctggtgc ccggcgatat tataaaactg
    65701 gcggcggggg atatgatccc ggcagattta cgtatcttgc aggcgcggga tctgttcgtc
    65761 gttcaggcgt cgttaaccgg tgagtctctg cccgtggaaa aagccgctac cactcgccag
    65821 ccggagcaca gcaatccgct ggagtgtgac accctgtgtt ttatgggcac caccgtggtg
    65881 agcggcacgg cacaagcaat ggtgattgct acaggagcca atacctggtt tggtcaactg
    65941 gcggggcgtg ttagtgagca ggaaagcgag ccgaatgcct ttcagcaggg gatcagccgc
    66001 gtcagtatgc tgctgattcg ctttatgctg gtgatggctc cggtggtgtt gcttatcaac
    66061 ggttacacca aaggcgactg gtgggaagcg gcactgtttg cgctttcggt cgcggtaggc
    66121 ttaacgccgg aaatgttgcc gatgattgtt acctcgacgc tggcgcgcgg cgcagtaaag
    66181 ctgtcgaaac agaaagtgat cgtcaaacat ctggatgcta ttcagaactt tggcgcaatg
    66241 gatattctgt gcactgataa aactggcacc ctgacacagg acaaaattgt gctggagaat
    66301 cataccgata tctccggtaa aaccagcgaa cgcgtgctgc atagcgcgtg gttgaacagt
    66361 cattatcaga ccggacttaa aaacctgctc gatacagcgg tgctcgaagg tacggatgaa
    66421 gagtcagcgc gctcgctggc cagtcgttgg caaaaaattg atgagattcc gtttgatttc
    66481 gagcgtcgtc ggatgtcggt ggtagtggca gaaaataccg agcaccatca gctggtttgc
    66541 aaaggtgcat tgcaggaaat cctcaatgtg tgttcgcagg tgcgtcacaa tggcgagatt
    66601 gtgccgctcg atgacattat gctgcgtaag attaagcggg ttactgatac gctgaatcgt
    66661 caggggctgc gcgtggttgc ggtggcgacg aaatacctgc cagcgcgtga aggagattac
    66721 cagcgggcgg atgaatccga cctgatcctc gaaggatata ttgctttcct tgatccgcca
    66781 aaagagacaa ccgctccggc actgaaggca ttaaaagcga gtggaatcac cgtaaaaatc
    66841 cttactggcg acagtgagtt agttgcagcg aaagtgtgcc atgaagtggg gctggacgcg
    66901 ggagaggtgg tcattggtag tgatattgaa acgctatctg acgacgaact ggcaaatctc
    66961 gcacagcgca ccacgctgtt tgcccgcctg acgccgatgc ataaagaacg cattgtgacc
    67021 ttactgaagc gcgaagggca tgtggttggc tttatgggcg atggtattaa tgatgcgccc
    67081 gccttacgcg ctgcggatat cggcatttct gtggacggcg cggtagatat tgcccgtgaa
    67141 gcggctgata tcatcctgct ggaaaaaagc ctgatggtgc tggaagaggg ggttattgaa
    67201 ggacgtcgca ctttcgccaa catgctgaaa tacatcaaaa tgacggcgag ctccaacttc
    67261 ggtaatgtgt tcagcgtgct ggtggcgagt gctttcttgc ccttcctgcc gatgttgccg
    67321 ttacacttgc ttattcagaa cctgctgtat gatgtgtcac aggtggcgat cccgtttgat
    67381 aacgtcgacg acgagcaaat tcaaaagccg cagcgttgga atccggcgga tctggggcgc
    67441 tttatgatct tcttcggacc gatcagctcg atcttcgata ttctgacgtt ttgcctgatg
    67501 tggtgggtat tccatgccaa cacgccggaa acgcaaacgc tgttccagtc gggatggttt
    67561 gtggtgggct tactgtcgca aacgctgatt gtgcatatga tccgcacccg ccgtgtgccg
    67621 tttattcaga gctgtgcatc gtggccgtta atgatcatga ccgtgatcgt gatgattgtc
    67681 gggatcgcat tgccgttttc accgctggcc agttatctgc agctgcaggc gctgccgtta
    67741 agctatttcc cgtggctggt tgcgattctg gcagggtata tgacattaac tcagttggtg
    67801 aaaggatttt atagccgtcg ttacggctgg cagtaaagaa taaactgggc acgatagccc
    67861 cgattcagat tgcagacaaa gtaagctttg ttcatgccgg atgcggcgta aatgccttat
    67921 ccggccaaca aattcaataa attgcaggaa tcacgtaggc ctgataagcg tagcgcatca
    67981 ggcgattttg cgtttgtcat cagacttaat ccgggcatga tagcccggat ttccatcaag
    68041 attagcgacg aacagcgatc gcttcgatct caatcttcac gtctttcggc agacgggcaa
    68101 cttcaacgca agaacgtgcc gggaaggtgg cgttgtgttc ggtgaagaag gcttcgtaag
    68161 tggcgtttac ggttgcgaag tcgttcagat ctttcacaaa cacggtggtt ttaacgatgt
    68221 cgcccacttt caggccagcg gcttcgacga ttgcttttac gttatccagc gactgacgtg
    68281 cctgtgctgc gacgtctgtc ggtacttcgc ccgttttcgg gtttaccggg atctgcccgg
    68341 aagtgatgat catgctgccc agatcaacgc cctgaacgta aggaccgata gctgccggtg
    68401 cattttccgt cgcgatagtt ttgctcatga tttctccttt attacagcgg taaaaagtct
    68461 ggctcattat agggagccag acttttatta ccaaccgcaa ttaattggcc agcaccacat
    68521 tatgggaaaa ctctttttca cagtatttgc atttgagcgc gatgtcattg gcgcgttttc
    68581 gcacggcaaa gctggaagaa accggttcgg catggctgat acagttgctg ttcgggcaga
    68641 ccagcacatt gccgatgcgc tctggcagac tggggcgcga tttacccacc acttcatagt
    68701 tgtcgatacg gttaaccgtg gcttgcggcg catacaatgc cagttgatct acctgatctt
    68761 cactcaaaaa ggtattttcg attttgatca gatctttgcg gcccatctcg ccagaaggca
    68821 agttcagacc gatggtgatg cgctgatccg tttcggtcag cttgaacaga ctcaacagct
    68881 taaaaccgat ctgggcgggg atatggtcaa ttaccgtgcc gcgtttaata gcttcaacct
    68941 gcaatttatt atcgtgtgtc atctctattt ccccttacag taccagatcg cgattcagaa
    69001 ccagtgccag taacgcctgg cgagcgaaaa tcccgttgcc tgcctgctgg aagtaccagg
    69061 cgtgtggcgt tttatcaaca tccgtcgcaa tctcatcaac acgcggcagc ggatgcagca
    69121 ctttcatatt ggctttggcg ttgtggagat cgctggcgcg aagaacaaac tgcgctttca
    69181 cgttggcgta ctcggacggg tccagacgct ctttttgcac gcgggtcatg tacaggatgt
    69241 ctacttccgc catcacttct tcaatagagc tgtgcagact ccatgcgatc cctttttcat
    69301 cgagcatatc cagaatgtat tgcggcattg ccagcgcgtc cggcgcgatg aagtaaaaac
    69361 ggttgccgtc gaacttcgct aacgcctgag tcagggagtg aacggtgcgg ccatatttca
    69421 ggtcaccaac cattgcgacg tggagattgt ccagacgccc ctgggtttcc tgaatagtga
    69481 ataagtccag caaggtttgc gtcggatgtt ggttggagcc atcaccggca ttcagtaccg
    69541 gtacattgcc ggaaaactcg gtggccaggc gcgccgcacc ttcctgcgga tgacgcatca
    69601 ctatcgcatc gacgtaagtg ctgataaccg aaatggtatc ggccagcgtt tcgccctttt
    69661 tacccagtga tgtattggcg ctgtcggaga agcccaccac gctggccccc aggcggtgca
    69721 tagatgtttc gaaagagagg cgggtacggg tagaggcttc gaagaaacag ctggcaatga
    69781 ctttgtgctt caacagctct ggttgcgggt ttgctttcag tttcgccgct gtcgccagca
    69841 ccagattaag gtcatcgcga ctaaggtcgt ttatggaaat gatatgtttc tgatatagcg
    69901 gattagccat cttttatctc ctgacgcctg ggcaaaaaaa agcccctcga ttgaggggct
    69961 gggaatgggt gatcaacggg aagaaaaacg gcaggccagc gtcttttttc agacgcggta
    70021 agacaaaatg tcgaacacac tgaaccatac atcctcccgg caaattgtcc ggcattatac
    70081 tcatcgtcag aagcggcgca agcatttgat gcaatatttt gtcagcgcaa acggtttatt
    70141 tgaattaaaa gtcaaggtat atgcattttt atttgatgtg attctgcagg ggaactgtcc
    70201 ttcggtatca ataattgcag acattccctg ctttttcctt tttcactttc acgcaatcag
    70261 atatgcattt tattccattc atccgactta tagggcggag tgtgaaagcg aacggctaac
    70321 actattgctt actgctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc
    70381 agccatgtaa cacccaccgc cgtccagttt ggataaggtg gggcgctaaa tatttcattt
    70441 ttcaccgtca tgatgtcttc aaattggttt tctggatcgg tatggaagct cgtaacatca
    70501 atgatatcgt caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaatgcc
    70561 agtctgactt gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc
    70621 ccggaaacaa acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatat
    70681 agtgaatgtc ggccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga
    70741 tttacgacat gaacagaaga tttctcttta ccgggagccg cttttagcgg acgacgtgag
    70801 taaacaaaac ccagacatca tggataatgg ctgggcttaa ttgagcgtag tcggttatgc
    70861 gccaaacgcg ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc
    70921 taaccatgcg accataccag cgacctctcc cggttgccca tgtcttttga tagccatcaa
    70981 actatgcaac atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc
    71041 tggctggacg acgttaatgg tgatcccacg cggtccaaaa tcacgggcca gcccgcgcgc
    71101 catgccttgc agggcagatt tgctggcggc ataagcagcc atgcctgcaa caggcatacg
    71161 atcgccattc acggagccga tgattaagat gcgcccgcct tcgggcatct gccgggcggc
    71221 ttcaacagag gcatgataag gagcatgaat attgattttg aaaaggcgat caatatcgtc
    71281 ggcatttaat tccagggcct cgccaaagac gccaatacct gcatttacca ccaggatatc
    71341 caatgcgccg ctcttacgaa cgacatcaat gacagcgtct ctgtcagcac tatctgtgaa
    71401 tactgctgtc gctccagtct cttgtgccag gcgtttagcg gcatctttcg accccgcata
    71461 ggtgaatcgt acattggccc catcggtgac gaaacgacgt acgatagcgg caccgatacc
    71521 acgactgcca ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg
    71581 atttattatg taacatgcat tacaaaactg ttttaacttt ctgtcaacag gttttgtaat
    71641 ggtcactaaa aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc
    71701 aatctctgcg gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga
    71761 agttactgat tatcttggta ttaacccccc gagcctctac gcggcttttg gcagtaaagc
    71821 tgggttattt agccgtgtac tcaatgaata cgtcggtacg gaagctattc cgcttgccga
    71881 tattcttcgt gatgatcgtc cagtaggcga gtgcctggtt gaggtattaa aagaagcggc
    71941 gcgcagatat agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gtattcatag
    72001 tcatgatcca ctagcgcgtg atattgccgt tcaatattat cacgccgcag aaacgaccat
    72061 ttatgactat atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag
    72121 taccgtgatg tcagggcttt ctgcgaaggc acgggagggg cactcaatcg aacaactctg
    72181 tgcaacagct gcactggcgg gggaagcgat aaaaactctt ctcaaggagt gatgctggcc
    72241 ttgatccgaa aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg
    72301 ttaaggctag cggaattgat catcttttcg tataacgata gaaatgaaac gttgttttaa
    72361 ttaaggagtg gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca
    72421 ggagttacgc caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg
    72481 caagcacgat atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc
    72541 gtacgaagcc cgccgtgcgg agtaccatgc ccgctatctc gacattcaga ttgtgttaaa
    72601 aggtcaggaa ggcatgacct tcagcacgca acctgcaggc acgccggata ccgactggtt
    72661 agctgataaa gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatccttaa
    72721 tgaaggtgat tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg
    72781 tgcaccagcc caggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc
    72841 cgtaaataac tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg
    72901 tgagtaaaat ttgtaatgct tagacgttct tattaactca aggagtccgt catgagcaaa
    72961 atatcaggtt ggaatttttc tcaaaacatt acatcagccg acaattgtaa acaaaaaaat
    73021 gaagacttag atacctggta tgtgggaatg aatgattttg cccgaattgc cggagggcag
    73081 aatagcagaa gcaatattct ttctcccaga gcatttttgg agtttttggc taagatattt
    73141 accctgggtt atgtggattt tagcaaacgc tccaacgaag cgggtagaaa tatgatggct
    73201 catattaagt cctcatctta tatcaaaaat aatgatggta gtgagataat gaagtttgtt
    73261 atgaataatc ctgaagggga gcgagcggat ttatctaagg tggaaataga aattacactt
    73321 gcctcagctt ttaataatgg gattcgtgaa ggacatacag tcattatatt tacacaacct
    73381 gatggttcga ctaatcgtta tgaagggaag tcctttgaaa gaaaagatga gagttcatta
    73441 cacctgatta ctaacaagat tctggcgtgt taccaacgcg aagctaacaa ggaaatagct
    73501 cgtctattaa atattccgca ggagttaaat aattctcagg acttgaataa ctcgcaggtg
    73561 agttgtaaag attcagttga ttctacgatt acggatttat tagaaaaacc attgaataat
    73621 gcattattag caataaggaa agaacatctg ctattaatgc cttatgtatg taatgaatcg
    73681 atttcatact tgcttggtga aaaag
//
LOCUS       DAZKIV010000006        70538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZKIV010000006 DAZKIV010000000
VERSION     DAZKIV010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 70538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.006"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(142..218)
                     /locus_tag="ACHOT6_000417"
     tRNA            complement(142..218)
                     /locus_tag="ACHOT6_000417"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(182..184),aa:Arg,seq:acg)
     gene            complement(282..358)
                     /locus_tag="ACHOT6_000418"
     tRNA            complement(282..358)
                     /locus_tag="ACHOT6_000418"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(322..324),aa:Arg,seq:acg)
     gene            complement(556..632)
                     /locus_tag="ACHOT6_000419"
     tRNA            complement(556..632)
                     /locus_tag="ACHOT6_000419"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(596..598),aa:Arg,seq:acg)
     gene            complement(636..728)
                     /locus_tag="ACHOT6_000420"
     tRNA            complement(636..728)
                     /locus_tag="ACHOT6_000420"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(692..694),aa:Ser,seq:gct)
     gene            complement(1044..1229)
                     /gene="csrA"
                     /locus_tag="ACHOT6_000421"
     CDS             complement(1044..1229)
                     /gene="csrA"
                     /locus_tag="ACHOT6_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFP9945703.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1464..4094)
                     /gene="alaS"
                     /locus_tag="ACHOT6_000422"
     CDS             complement(1464..4094)
                     /gene="alaS"
                     /locus_tag="ACHOT6_000422"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFP9945704.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4222..4722)
                     /gene="recX"
                     /locus_tag="ACHOT6_000423"
     CDS             complement(4222..4722)
                     /gene="recX"
                     /locus_tag="ACHOT6_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFP9945705.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4965..6026)
                     /gene="recA"
                     /locus_tag="ACHOT6_000424"
     CDS             complement(4965..6026)
                     /gene="recA"
                     /locus_tag="ACHOT6_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFP9945706.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(6106..6603)
                     /gene="pncC"
                     /locus_tag="ACHOT6_000425"
     CDS             complement(6106..6603)
                     /gene="pncC"
                     /locus_tag="ACHOT6_000425"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFP9945707.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6748..7833)
                     /gene="mltB"
                     /locus_tag="ACHOT6_000426"
     CDS             complement(6748..7833)
                     /gene="mltB"
                     /locus_tag="ACHOT6_000426"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFP9945708.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            8089..8652
                     /gene="srlA"
                     /locus_tag="ACHOT6_000427"
                     /gene_synonym="gutA"
     CDS             8089..8652
                     /gene="srlA"
                     /locus_tag="ACHOT6_000427"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFP9945709.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8649..9608
                     /gene="srlE"
                     /locus_tag="ACHOT6_000428"
                     /gene_synonym="gutE"
     CDS             8649..9608
                     /gene="srlE"
                     /locus_tag="ACHOT6_000428"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFP9945710.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9619..9990
                     /gene="srlB"
                     /locus_tag="ACHOT6_000429"
                     /gene_synonym="gutB"
     CDS             9619..9990
                     /gene="srlB"
                     /locus_tag="ACHOT6_000429"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFP9945711.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVSDDIAPGSVLKFESVKE"
     gene            9994..10773
                     /gene="srlD"
                     /locus_tag="ACHOT6_000430"
     CDS             9994..10773
                     /gene="srlD"
                     /locus_tag="ACHOT6_000430"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFP9945712.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10878..11237
                     /gene="gutM"
                     /locus_tag="ACHOT6_000431"
     CDS             10878..11237
                     /gene="gutM"
                     /locus_tag="ACHOT6_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFP9945713.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGTHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11304..12077
                     /gene="srlR"
                     /locus_tag="ACHOT6_000432"
                     /gene_synonym="gutR"
     CDS             11304..12077
                     /gene="srlR"
                     /locus_tag="ACHOT6_000432"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFP9945714.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            12070..13035
                     /gene="gutQ"
                     /locus_tag="ACHOT6_000433"
     CDS             12070..13035
                     /gene="gutQ"
                     /locus_tag="ACHOT6_000433"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFP9945715.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(13032..14546)
                     /gene="norR"
                     /locus_tag="ACHOT6_000434"
     CDS             complement(13032..14546)
                     /gene="norR"
                     /locus_tag="ACHOT6_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFP9945716.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPTPEVAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14733..16172
                     /gene="norV"
                     /locus_tag="ACHOT6_000435"
     CDS             14733..16172
                     /gene="norV"
                     /locus_tag="ACHOT6_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFP9945717.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            16169..17302
                     /gene="norW"
                     /locus_tag="ACHOT6_000436"
     CDS             16169..17302
                     /gene="norW"
                     /locus_tag="ACHOT6_000436"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFP9945718.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17430..19682)
                     /gene="hypF"
                     /locus_tag="ACHOT6_000437"
     CDS             complement(17430..19682)
                     /gene="hypF"
                     /locus_tag="ACHOT6_000437"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFP9945719.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGKIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKHVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECQHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19835..20362)
                     /gene="hydN"
                     /locus_tag="ACHOT6_000438"
     CDS             complement(19835..20362)
                     /gene="hydN"
                     /locus_tag="ACHOT6_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFP9945720.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20511..21521)
                     /gene="ascG"
                     /locus_tag="ACHOT6_000439"
     CDS             complement(20511..21521)
                     /gene="ascG"
                     /locus_tag="ACHOT6_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFP9945721.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            21781..23238
                     /gene="ascF"
                     /locus_tag="ACHOT6_000440"
                     /pseudo
     CDS             21781..23238
                     /gene="ascF"
                     /locus_tag="ACHOT6_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
     gene            23247..24671
                     /gene="ascB"
                     /locus_tag="ACHOT6_000441"
     CDS             23247..24671
                     /gene="ascB"
                     /locus_tag="ACHOT6_000441"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFP9945722.1"
                     /translation="MSVFPQGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     ELTPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARIFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVFVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24785..25204)
                     /locus_tag="ACHOT6_000442"
     CDS             complement(24785..25204)
                     /locus_tag="ACHOT6_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9945723.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(25201..25512)
                     /locus_tag="ACHOT6_000443"
     CDS             complement(25201..25512)
                     /locus_tag="ACHOT6_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP9945724.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            complement(25685..26446)
                     /locus_tag="ACHOT6_000444"
     CDS             complement(25685..26446)
                     /locus_tag="ACHOT6_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945725.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWHFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFNSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLNPIAGRDPQTEDLAQTQRCLIKGDVLEG"
     gene            complement(26471..26941)
                     /gene="hycI"
                     /locus_tag="ACHOT6_000445"
     CDS             complement(26471..26941)
                     /gene="hycI"
                     /locus_tag="ACHOT6_000445"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFP9945726.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(26934..27344)
                     /gene="hycH"
                     /locus_tag="ACHOT6_000446"
     CDS             complement(26934..27344)
                     /gene="hycH"
                     /locus_tag="ACHOT6_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFP9945727.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(27341..28108)
                     /gene="hycG"
                     /locus_tag="ACHOT6_000447"
     CDS             complement(27341..28108)
                     /gene="hycG"
                     /locus_tag="ACHOT6_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFP9945728.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHNLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDYLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(28108..28650)
                     /gene="hycF"
                     /locus_tag="ACHOT6_000448"
     CDS             complement(28108..28650)
                     /gene="hycF"
                     /locus_tag="ACHOT6_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFP9945729.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(28660..30369)
                     /gene="hycE"
                     /locus_tag="ACHOT6_000449"
     CDS             complement(28660..30369)
                     /gene="hycE"
                     /locus_tag="ACHOT6_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFP9945730.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(30387..31310)
                     /gene="hycD"
                     /locus_tag="ACHOT6_000450"
     CDS             complement(30387..31310)
                     /gene="hycD"
                     /locus_tag="ACHOT6_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFP9945731.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(31313..33139)
                     /gene="hycC"
                     /locus_tag="ACHOT6_000451"
     CDS             complement(31313..33139)
                     /gene="hycC"
                     /locus_tag="ACHOT6_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFP9945732.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSDAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGSAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(33136..33747)
                     /gene="hycB"
                     /locus_tag="ACHOT6_000452"
     CDS             complement(33136..33747)
                     /gene="hycB"
                     /locus_tag="ACHOT6_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFP9945733.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(33872..34333)
                     /gene="hycA"
                     /locus_tag="ACHOT6_000453"
     CDS             complement(33872..34333)
                     /gene="hycA"
                     /locus_tag="ACHOT6_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFP9945734.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            34545..34895
                     /gene="hypA"
                     /locus_tag="ACHOT6_000454"
     CDS             34545..34895
                     /gene="hypA"
                     /locus_tag="ACHOT6_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9945735.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            34899..35771
                     /gene="hypB"
                     /locus_tag="ACHOT6_000455"
     CDS             34899..35771
                     /gene="hypB"
                     /locus_tag="ACHOT6_000455"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFP9945736.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCLACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            35762..36034
                     /gene="hypC"
                     /locus_tag="ACHOT6_000456"
     CDS             35762..36034
                     /gene="hypC"
                     /locus_tag="ACHOT6_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFP9945737.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            36034..37155
                     /gene="hypD"
                     /locus_tag="ACHOT6_000457"
     CDS             36034..37155
                     /gene="hypD"
                     /locus_tag="ACHOT6_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFP9945738.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            37152..38162
                     /gene="hypE"
                     /locus_tag="ACHOT6_000458"
     CDS             37152..38162
                     /gene="hypE"
                     /locus_tag="ACHOT6_000458"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFP9945739.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFIEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRNIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIELTEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            38236..40314
                     /gene="flhA"
                     /locus_tag="ACHOT6_000459"
     CDS             38236..40314
                     /gene="flhA"
                     /locus_tag="ACHOT6_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFP9945740.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(40351..40704)
                     /gene="ygbA"
                     /locus_tag="ACHOT6_000460"
     CDS             complement(40351..40704)
                     /gene="ygbA"
                     /locus_tag="ACHOT6_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFP9945741.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(40736..40915)
                     /locus_tag="ACHOT6_000461"
     CDS             complement(40736..40915)
                     /locus_tag="ACHOT6_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945742.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            40991..43552
                     /gene="mutS"
                     /locus_tag="ACHOT6_000462"
     CDS             40991..43552
                     /gene="mutS"
                     /locus_tag="ACHOT6_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFP9945743.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            43658..44314
                     /gene="pphB"
                     /locus_tag="ACHOT6_000463"
     CDS             43658..44314
                     /gene="pphB"
                     /locus_tag="ACHOT6_000463"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9945744.1"
                     /translation="MPSTRYQKINTHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK
                     "
     gene            complement(44365..45132)
                     /gene="ygbI"
                     /locus_tag="ACHOT6_000464"
     CDS             complement(44365..45132)
                     /gene="ygbI"
                     /locus_tag="ACHOT6_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFP9945745.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            45328..46236
                     /gene="ltnD"
                     /locus_tag="ACHOT6_000465"
     CDS             45328..46236
                     /gene="ltnD"
                     /locus_tag="ACHOT6_000465"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFP9945746.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            46233..46793
                     /locus_tag="ACHOT6_000466"
                     /pseudo
     CDS             46233..46793
                     /locus_tag="ACHOT6_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417217.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
     gene            46789..47319
                     /locus_tag="ACHOT6_000467"
                     /pseudo
     CDS             46789..47319
                     /locus_tag="ACHOT6_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="member of the Gnt family of gluconate transporters;
                     not involved in gluconate transport; unknown function;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
     gene            complement(47359..47595)
                     /locus_tag="ACHOT6_000468"
     CDS             complement(47359..47595)
                     /locus_tag="ACHOT6_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFP9945747.1"
                     /translation="MICPRCADEQIEVMVKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLAEGKR"
     gene            complement(47606..49033)
                     /locus_tag="ACHOT6_000469"
     CDS             complement(47606..49033)
                     /locus_tag="ACHOT6_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFP9945748.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVAMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRSKPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTTMLAARK"
     gene            complement(49033..49626)
                     /locus_tag="ACHOT6_000470"
     CDS             complement(49033..49626)
                     /locus_tag="ACHOT6_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFP9945749.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALRDMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            49773..50180
                     /locus_tag="ACHOT6_000471"
     CDS             49773..50180
                     /locus_tag="ACHOT6_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFP9945750.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(50301..51293)
                     /gene="rpoS"
                     /locus_tag="ACHOT6_000472"
     CDS             complement(50301..51293)
                     /gene="rpoS"
                     /locus_tag="ACHOT6_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFP9945751.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(51356..52441)
                     /gene="nlpD"
                     /locus_tag="ACHOT6_000473"
     CDS             complement(51356..52441)
                     /gene="nlpD"
                     /locus_tag="ACHOT6_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFP9945752.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVAQQPVQMENGRIVYNRQYGNIPKGSYSG
                     STYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGGNA
                     ITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTAPV
                     TVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAIIA
                     TADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMGST
                     GTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(52626..53252)
                     /gene="pcm"
                     /locus_tag="ACHOT6_000474"
     CDS             complement(52626..53252)
                     /gene="pcm"
                     /locus_tag="ACHOT6_000474"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFP9945753.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(53246..54007)
                     /gene="surE"
                     /locus_tag="ACHOT6_000475"
     CDS             complement(53246..54007)
                     /gene="surE"
                     /locus_tag="ACHOT6_000475"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFP9945754.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(53988..55037)
                     /gene="truD"
                     /locus_tag="ACHOT6_000476"
     CDS             complement(53988..55037)
                     /gene="truD"
                     /locus_tag="ACHOT6_000476"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFP9945755.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(55034..55513)
                     /gene="ispF"
                     /locus_tag="ACHOT6_000477"
     CDS             complement(55034..55513)
                     /gene="ispF"
                     /locus_tag="ACHOT6_000477"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFP9945756.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(55513..56223)
                     /gene="ispD"
                     /locus_tag="ACHOT6_000478"
     CDS             complement(55513..56223)
                     /gene="ispD"
                     /locus_tag="ACHOT6_000478"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFP9945757.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(56242..56553)
                     /gene="ftsB"
                     /locus_tag="ACHOT6_000479"
     CDS             complement(56242..56553)
                     /gene="ftsB"
                     /locus_tag="ACHOT6_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFP9945758.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(56747..57070)
                     /gene="ygbE"
                     /locus_tag="ACHOT6_000480"
     CDS             complement(56747..57070)
                     /gene="ygbE"
                     /locus_tag="ACHOT6_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFP9945759.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(57120..57725)
                     /gene="cysC"
                     /locus_tag="ACHOT6_000481"
     CDS             complement(57120..57725)
                     /gene="cysC"
                     /locus_tag="ACHOT6_000481"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFP9945760.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(57725..59152)
                     /gene="cysN"
                     /locus_tag="ACHOT6_000482"
     CDS             complement(57725..59152)
                     /gene="cysN"
                     /locus_tag="ACHOT6_000482"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFP9945761.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARIDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(59154..60062)
                     /gene="cysD"
                     /locus_tag="ACHOT6_000483"
     CDS             complement(59154..60062)
                     /gene="cysD"
                     /locus_tag="ACHOT6_000483"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFP9945762.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            60314..61351
                     /gene="iap"
                     /locus_tag="ACHOT6_000484"
     CDS             60314..61351
                     /gene="iap"
                     /locus_tag="ACHOT6_000484"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFP9945763.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLVVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   61433..62013
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=61433..61461
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(62108..62332)
                     /gene="cas2e"
                     /locus_tag="ACHOT6_000485"
     CDS             complement(62108..62332)
                     /gene="cas2e"
                     /locus_tag="ACHOT6_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFP9945764.1"
                     /translation="MEVRAGVYVGDTSKRIREMIWQQITQLAGCGNVVMAWATNTESG
                     FEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(62401..63321)
                     /gene="cas1e"
                     /locus_tag="ACHOT6_000486"
     CDS             complement(62401..63321)
                     /gene="cas1e"
                     /locus_tag="ACHOT6_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFP9945765.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(63318..63967)
                     /gene="cas6e"
                     /locus_tag="ACHOT6_000487"
                     /pseudo
     CDS             complement(63318..63967)
                     /gene="cas6e"
                     /locus_tag="ACHOT6_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
     gene            complement(63949..64695)
                     /gene="cas5e"
                     /locus_tag="ACHOT6_000488"
     CDS             complement(63949..64695)
                     /gene="cas5e"
                     /locus_tag="ACHOT6_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFP9945766.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPCWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(64706..65761)
                     /gene="cas7e"
                     /locus_tag="ACHOT6_000489"
     CDS             complement(64706..65761)
                     /gene="cas7e"
                     /locus_tag="ACHOT6_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFP9945767.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEEKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRMAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            complement(65773..66309)
                     /gene="casB"
                     /locus_tag="ACHOT6_000490"
                     /gene_synonym="cse2"
     CDS             complement(65773..66309)
                     /gene="casB"
                     /locus_tag="ACHOT6_000490"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFP9945768.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMSKGRFTRLS
                     AVKTPDELLRQLRRAVRLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQNQ"
     gene            complement(66306..67868)
                     /gene="casA"
                     /locus_tag="ACHOT6_000491"
                     /gene_synonym="cse1"
     CDS             complement(66306..67868)
                     /gene="casA"
                     /locus_tag="ACHOT6_000491"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFP9945769.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDISAPRADL
                     QGAVWQFLLGLLQTSFAPKDHRRWDDIWEDGLEAEKLREALQSLEHAFQFGPDSPSFM
                     QDFEALTGDKVPVASLLPEIPGAQTTKFNKDHFIKRGVTEYLCPHCSALALFSLQLNA
                     PSGGKGYRTGLRGGGPMTTLIELQEYQGNQQTPLWRKLWINVMPQDEADLPLPKKFDD
                     LVFPWLGPTRTSELAGAVVTDEQVNKLQAYWGMPRRIRIDFNTTTVGNCDICGEQSDA
                     LLSLMTTKNYGANYAMWQHPLTPYRVPLKEGGEFYSVKPQPGGLIWRDWLGLIETGKS
                     ENNTELPALVVKLFNASSLKQAKVGLWGFGYDFDNMKARCWYEHHFPLLLDKKEGQIP
                     KLRLAAQTASRILSLLRSALKEAWFSDPKGARGDFSFVDIDFWNKTQHRFLRLVRQIE
                     EGQDADELLSKWNKEIWLFARQDFDERVFTNPYEPVDLERVMTARKKYFTTSAEKQSA
                     KAAREKKQEAAE"
     gene            complement(67966..>70538)
                     /gene="cas3"
                     /locus_tag="ACHOT6_000492"
     CDS             complement(67966..>70538)
                     /gene="cas3"
                     /locus_tag="ACHOT6_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HFP9945770.1"
                     /translation="DVAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHD
                     IGKFARSFQQLYLPPELKIQEGARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSP
                     RKLRRVIEMWMPVTTGHHGRPPDRMDELDNFLPEDKAAARDFLLEIKPLFPLIEIPAF
                     WDDDEGIELIKHLSWYISATVVLADWTGSSTRFFPRVAHPMDIKGYWQKTLIQAQNAL
                     TVFPLKAKVAPFNGINTLFPFIENPTPLQQKVLDLDISQQGPQLFILEDVTGAGKTEA
                     ALILAHRLIAAGKAQGLFFGLPTMATANAMYDRLVKTWLAFYSPESRPSLVLAHSART
                     LMDRFNESLWSGDLVGSEEPDEQTFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVM
                     PFKHNNLRLLGLSNKILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQ
                     RDKLVAAFARGTEGQQEAPFLEKDDYPWLTHVTKSDVNSHRVATRKDVERSVSVGWLH
                     SEQECIARIESAVSQGKCIAWIRNSVDDAIKVHRQLLARGVIPASSLSLFHSRFAFSD
                     RQRIETETLARFGKYCSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPVDLLIQRAGR
                     LQRHIRDINGQLKRDGKDERSPPELLILAPVWDDSPGDEWFGSAMRNSAFVYPDHGRI
                     WLTQRVLREQGAIQMPHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRAMAKKF
                     VLNFRPGYAANINDYLPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRV
                     RRSWWKKHRDEFSLLEGEAFRLWCIEQRQDPEMANVILVNDDESCGYSATEGLIGKVG
                     "
BASE COUNT        16647 a        19367 c        18054 g        16470 t
ORIGIN      
        1 aagagatggt gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta
       61 aagcaacgtt atcatccctg gtgcctgcgg ttaattccgc tacctcttcg ataccttcat
      121 tgctgaagat ttcggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt
      181 tcgtagccga gtactctatc cagctgagct acggatgcat cgggaaaatt tattttactg
      241 ctgatattga taccgctact aaagccatat caagtaagag atggtgcatc cgggaggatt
      301 cgaacctccg accgctcggt tcgtagccga gtactctatc cagctgagct acggatgcat
      361 cgggaaactt actttactgc agattttttg ataccgctac taaagccgta tcaagtaaga
      421 gatggtgcat ccgggaggat tactcgcctt gcggctcgcc ctttgggccg ttgctaaagc
      481 aacgttatcc tccctggtgc ctgcggttat accgcaacct cttccatacc ttcatcgctg
      541 aaagtacgta gaatatggtg catccgggag gattcgaacc tccgaccgct cggttcgtag
      601 ccgagtactc tatccagctg agctacggat gcaaatggcg gtgaggcggg gattcgaacc
      661 ccggatgcag cttttgaccg catactccct tagcagggga gcgccttcag cctctcggcc
      721 acctcaccac acgcctctta cgagtgcttc gaagaacttg tttatcgctc atcgtcgctg
      781 cgtggcgcac atattacttt ctgagactta taagtcaaac aatttttcct acacttttat
      841 cgtttgcaca cttcacgttc aattagtctg taaaaacgtc aaaaagagtg ttttatcaac
      901 agaaaaatgg aggtctgaca gatagtagta atgcaaaaaa atggagactt aagttgaatg
      961 aacgggagta aagcgaaaag actatagagt gaaggagaaa ttttgagggt gcgtctcacc
     1021 gataaagatg agacgcggaa agattagtaa ctggactgct gggatttttc agcctggata
     1081 cgctggtaga tctcttcacg gtgaacagaa acttccttcg gggcatttac gccaatacgt
     1141 acctggttgc cctttacccc taaaactgtc acggtgacct catccccaat catgagggtc
     1201 tcaccaactc gacgagtcag aatcagcatt ctttgctcct tgaaagatta aaagagtcgg
     1261 gtctctctgt atcccggcat tatccatcat ataacgccaa aaagtaagca atgacaaaca
     1321 cattacatgt aagcagtcat ggcattacat tctgttaaac ctaagtttag ccgatataca
     1381 caacttcaac ctgactttat cgttgtcgat agcgttaatg cgaatgccgt gaagcgagtc
     1441 cacggcattg cctgacgctt atattattgc aatttcgcgc tgacccagcc tttcacactg
     1501 gctaacgctg caggtaaggc cgcagcatcc gtaccaccgg cttgcgccat gtcaggacgt
     1561 ccaccaccct tgccgcccac ctgctgagcg accataccaa tcagttcccc tgctttcaca
     1621 cgatctgtga cgtccttaga tacgcctgca atcagagaaa ccttaccttc ggctaccgtt
     1681 gccagcacga taattgtcga ccccagctga ttttttaaat cgtcaaccat ggtacgcaac
     1741 attttcggct caacaccgct aagctcgcta accaacagct taacaccatt aacatcaatt
     1801 gccttactgg aaagatttgc gctctcctgt gcggcagctt gttctttaag ctgttgtagt
     1861 tctttttcta actgacgcgt acgttccagt actgagcgca ctttatccgc cagattattg
     1921 ctatcgcctt tcagcagatg cgcgacttcg cttaagcgat cgctgtctgc atgaacggtg
     1981 gtgatagcgc cttctccggt taccgcttcg atacgacgaa cgcctgcagc agtacccgat
     2041 tcagagatga tgcggaacag accaatatca ccagtgcggc tggcgtgagt accgccacac
     2101 agctcggtgg agaaatcgcc catgctcagc acgcgtacac gctcatcata cttctcgccg
     2161 aacagcgcca tcgcaccttt cgctttcgcc gcttcgagat ccatgatgtt ggtttcgatc
     2221 ggcaaattgc gacgaatctg tgcgttcacc aggtcttcga ccgcacgaat ctcttccggt
     2281 ttcatcgctt cgttgtgtga gaagtcgaag cgcagcacct tgtcgttaac cagtgaacct
     2341 ttctgcgata catgagtccc cagaacctgg cgcagcgcag cgtgcatcag gtgcgttgcg
     2401 gagtgattca gacgaatacg ggcgcgacga gcctcatcaa catcagcctg cacagcgtcg
     2461 cccactttca gagaacccgc agcaagttta ccgatgtgac caatcgcctg gccgtatttc
     2521 tgcgtatctt ccaccgcgaa ggagaagtta gcgcctttca gttcgccttt atcaccaacc
     2581 tgaccgccgg attccgcata gaatggtgtt tgatccagca cgaccacagc ttcctggcct
     2641 gcattgatgg catcaaccgc tttaccatca acaaacagcg cggtcacttt gccgttcagt
     2701 tccagatggt catagccttt aaattcagat gcactatcaa cacggatcat tgcgttgtaa
     2761 tcggcaccaa agccgctggc ttcgcgcgcg cgacgacgct gctcttccat tgctgcttca
     2821 aaaccagctt cgtcaacttt gatgttgcgc tcacgacaaa catcagccgt caggtcaacc
     2881 gggaagccat aggtgtcgta cagacggaaa gccgtttcac catccagcgt atcaccagaa
     2941 agttttgcca gctcttcatc cagcaacgcc agaccgcgct ccagagtacg ggcaaactgc
     3001 tcttcttcag tcttcagcac ctgctcaacc tgcgcctgct ggcgtttcag gtcttcaccc
     3061 gcagagccca taacgtcgat cagcggacca accagtttgt agaagaaggt ttctttcgcg
     3121 ccgagcatat tgccgtgacg cactgcgcga cgaatgatac gacgcagtac ataaccacgg
     3181 ttttcattgg acggcattac gccatccgcg atcaggaacg cacaagaacg aatgtggtca
     3241 gcgattacgc gcagcgattt attgctcaga tcggtcgcgc cagtgacttt cgctaccgcc
     3301 tggatcaacg tgcggaacag gtcgatgtca tagttagagt taacgtgttg cagcaccgca
     3361 gcaatacgct ccagacccat accggtatct acagacggct tcggcagcgg ttccatcgtg
     3421 ccatctgcct ggcggttgaa ctgcatgaag acgatgttcc agatctcaat gtagcggtcg
     3481 ccgtcttctt ccggacttcc cggagggcca ccccaaatgt ggtcgccgtg atcgtagaag
     3541 atttcggtgc acgggccgca cggaccagtg tcacccatct gccagaagtt gtcagatgcg
     3601 tatggcgcac ctttgttatc gccgatgcga ataatacgtt cgcgcgggat cccgacttct
     3661 ttttcccaga tttcgtaggc ttcgtcgtcg ctttcataga cggtaaccca cagacgctct
     3721 ttcggcaggg caaaccattt ttcgctggtc agcagttccc atgcaaactg aatggcatcg
     3781 tgtttgaaat agtcgccgaa gctgaagttg cccagcattt cgaagaaggt atggtgacgc
     3841 gcggtgtaac cgacgttttc caggtcgttg tgtttaccac ccgcacgcac gcagcgttgg
     3901 gaagtggtag cgcgggaata attacgcttg tcgagcccaa ggaacacatc cttgaactgg
     3961 ttcatcccgg cgttggtaaa caacaaagtt gggtcgttat ggggtaccag ggagctgctg
     4021 gcaactacct gatgtccctt actatggaaa aagtcgagaa acgcctgacg gatctcagcg
     4081 gtgctcttgc tcataattat cctgaaatca agctaacgaa atatcgccac cagctccagc
     4141 gtgtcttaac cgccgggctg gtaactgaaa agtgggaata agataagttt tcttgactgg
     4201 gaagtaaaat accgtatgcg ttcagtcggc aaaatttcgc caaatatcct ggatatcttc
     4261 catcagatag ccacgataga gcagaaaacg ctggatctta actttttctg aaaagacagt
     4321 tggcaaaggt tcaccatatt ttcgcgtcgc ctgatcgcgc gccagtgcgc accagtcgat
     4381 gtcacattca cgcatcgctt tttctgtcgc ttcgcgggaa atacctttct gattcagttc
     4441 ctgacgaata cgtgcaggtc cataaccttt gcggctacgg ctggcgataa agcgcgcaac
     4501 aaatcggctg tcatcgagat agccatgttc atggcaccag gcaataacgc gctcgtaatc
     4561 ttctgccgta gcatcaatct cttctgggcc atttttgccc ataatcggtg ccgcgagttt
     4621 acgtcgcagt tcttgctcac tgtgatcgcg caccgccaga atgcgtaccg cacgatccaa
     4681 caggcgagca tatgccgggc gacgggacgt tgattctgtc atggcatatc cttacaactt
     4741 aaaaaagcaa aagggccgca gatgcgaccc aggttttgct gataaacaga tttaatagtc
     4801 aatgagtgtg cagaatgttc ccgcaggccc aattcgttag aggatcgagg tgagagtaat
     4861 ttttcacgcc agtgctggcc tgttcccctc accctaaccc tctccccaaa ggggcgaggg
     4921 gaccgtccgt gcacacatta tctattatca tcaataacga acgattaaaa atcttcgtta
     4981 gtttctgcta cgccttcgct gtcatctaca gagaaatccg gcgttgagtt cgggttgctc
     5041 agcagcaact cacgtacttt cttctcgatc tctttcgcgg tttccgggtt atctttcagc
     5101 caggcagttg cattcgcttt accctgaccg atcttctcac ctttgtagct gtaccacgcg
     5161 cctgctttct cgatcagctt ctcttttacg cccaggtcaa ccagttcgcc gtagaagttg
     5221 ataccttcgc cgtagaggat ctgaaattca gcctgtttaa acggtgcagc gattttgttc
     5281 ttcaccactt tcacgcgggt ttcgctaccc accacgtttt cgccctcttt caccgcgccg
     5341 atacgacgga tgtcgagacg aacagaggcg tagaatttca gcgcgttacc accggtagtg
     5401 gtttccgggt taccgaacat cacaccaatt ttcatacgga tctggttgat gaagatcagc
     5461 agcgtgttgg actgcttcag gttacccgcc agcttacgca tcgcctggct catcatacgt
     5521 gccgcaaggc ccatgtgaga gtcgccgatt tcgccttcga tttccgcttt cggcgtcagt
     5581 gccgccacgg agtcaacgac gataacgtct actgcgccag aacgcgccag ggcgtcacag
     5641 atttccagtg cctgctcgcc ggtgtccggc tgggagcaca gcaggttatc gatatcgacg
     5701 cccagtttac gtgcgtagat tgggtccagc gcgtgttcag catcgataaa cgcacaggtt
     5761 ttaccttcac gctgcgctgc ggcgatcacc tgcagcgtca gcgtggtttt accggaagat
     5821 tccggtccgt agatttcgac gatacggccc atcggcagac cacctgcccc aagcgcgata
     5881 tccagtgaaa gcgaaccggt agagatggtt tccacatcca tggaacggtc ttcacccagg
     5941 cgcatgatgg agcctttacc aaattgtttc tcaatctggc ccagtgctgc cgccaacgct
     6001 ttctgtttgt tttcgtcgat agccattttt actcctgtca tgccgggtaa taccggatag
     6061 tcaatatgtt ctgttgaagc aattatactg tatgttcata cagcatcaag tgttttgtag
     6121 aaattgttgc cacaaggtct gcaatgcata cgcagtagcc tgacgacgca ccgcatcacg
     6181 gtcgccgctg aagcattccc gccgggtaat gccttcaccg cgggcagtgg caaaagcaaa
     6241 ccagacggtg ccgacaggct tctcttcact gccgccatcc ggcccggcga taccactaat
     6301 agacacggca taatcagcac gagccgcttt cagtgcgcct atcaccattt ccaccacgac
     6361 gggttcactc accgcgccat gctgcgccag cgtctcttcg cgtacgccga tcatctgcgc
     6421 tttggcttcg ttactgtagg tgacaaatcc gcgttcaaac caggcggagc taccggcaat
     6481 atcggtaatc actttcgcta cccaaccacc ggtacaagac tcggcagttg ttacggttgc
     6541 gccacgggct ttcagcgcct gcccaacctg ttcacttaac tgcatcagtt cactgtcagt
     6601 catcacaacc tctgtcagtc aaaaatttat gccggacaag atagcacttt cgtcaggaag
     6661 atggtgttgg cgttgcaatt ttacgaggag gattcagaaa aaagctgatt agccagaggg
     6721 aagctcacgc ccccctcttg caaatagtta ctgcacgcgc gccagcgcca cggcttgtcc
     6781 taactgccag accgccattg cgtaatgggt gctgtggtta taacgggtaa tggtgtagaa
     6841 gttcggcaga ccgtaccagt actggtaacc ggtaccgaca tccagacgca gcaggctggc
     6901 ttgttgatgg ttgcccagcg gctgctgtgg cgttaaacct gccgtggcaa gctgcgaaat
     6961 gctgtacctg gttttgaagc catttggcaa gcctggagcc tgaccgtttg ccattaccgc
     7021 gacctgatcg cctttcaccc agccgtgcgc tttgaaatag ttcgccacgc taccgatcgc
     7081 atcaaccgga tcccacaggt tgatatgccc gtcgccgctg aaatctaccg catattgttt
     7141 gtaagacgac ggcataaact gtccgtagcc catcgccccg gcaaaggaac ctttcagatt
     7201 gagcggatcg tcctgctcgt cgcgcgccat caacaggaag gtttccagct cgccagagaa
     7261 atactccgcg cggcgtgggt agttaaatga cagcgttgcc agcgcatcga ggatgcgagt
     7321 tttccccatc acgcgccccc agcgggtttc aacgccgata atcccgacga taatttccgg
     7381 cggtacgcca tacacctgcc acgcgcgatt caacgcatct tcatactgat tccagaaaac
     7441 cacaccgttc tgcacgttgt ccggcgtaat aaattttttg cgataacgga gccatgcgcc
     7501 gttcggacct gatggcggtt tcaccgatgt ggttggtgcc tggttatcca tcagccgcag
     7561 taccgaatcc agacgcttcg cctgggagag aatttcctgc aactgctgac gatcgaaacc
     7621 gtgtttgttc accattttgt cgatgaactg ctgggcattc gggttattag cgaaatcgcc
     7681 gcccatctgc atcacattgt gctgcggctc aagcaggaag ccgccagacg gcgttccggt
     7741 ggtcgtttca gtctcagtag gttttggctt gctgctacag gcggcaagca acacaaaaag
     7801 gggaagcaat gttacataac gacgcttgaa catgaggggt ccatttaaca gattcaacca
     7861 ggggcaagta tggtaaagca tcacgcccca cacaaggaag cggtagtcac tgcccgatac
     7921 ggactttaca taactcaact cattcccctc gctatccttt tacttaaact ttcaaattaa
     7981 aatatttatc tttcattttg agatcaagat aacactttta aatctttcaa tctgattaga
     8041 ttaggttgcc gtttggtaat aaaacaataa atcctgaagg agagaacaat gatagaaacc
     8101 attactcatg gtgcagagtg gtttatcggg ctgttccaaa agggcggaga ggtgtttacc
     8161 gggatggtga ccggcattct tccgctgttg attagcctgc tggttatcat gaacgcactg
     8221 attaatttta tcggtcagca tcgtattgaa cgttttgctc aacgttgcgc cggtaaccct
     8281 gtttcccgtt acctactgtt accgtgcatt ggcacgtttg tcttttgcaa tccgatgacc
     8341 ttaagcctgg gtcgctttat gccggaaaag tacaaaccca gctactacgc ggcggcctct
     8401 tatagctgcc actcaatgaa tggcctcttc ccccatatca accctggcga actgtttgtt
     8461 tatcttggca ttgccagcgg tctgacaacg ctgaacctgc cacttggccc actggcggtg
     8521 agttatctgc tggttggtct ggtcaccaat ttcttccgcg gctgggtgac cgatctgacc
     8581 accgccattt ttgagaaaaa gatgggcatt caacttgaac aaaaagttca cctggcagga
     8641 gcaacatcat gacgcatatt cggatcgaaa aaggaacggg tggctggggc ggcccgcttg
     8701 agctgaaagc cacgccggga aaaaaaatcg tctatatcac cgccggtacc cggcctgcga
     8761 ttgttgacaa actggcacag cttactggct ggcaggctat tgacggattt aaagaaggtg
     8821 aacccgcgga ggcggaaatt ggtgtcgcgg taatcgactg tggcggcaca ttacgctgcg
     8881 gcatctatcc gaaacgacgt attcccacca ttaatatcca ctcgacgggc aagtccggtc
     8941 cgctggcgca gtacattgtg gaagatattt atgtctctgg cgtaaaagaa gaaaacatca
     9001 ctgtagtggg tgatgcgaca ccacaaccct cttccgtggg ccgtgactat gacaccagta
     9061 agaaaatcac cgaacaaagc gatggtttac tggcgaaggt gggaatgggc atggggtccg
     9121 ccgttgcggt gctgtttcaa tctggtcgtg acaccatcga cactgtatta aaaaccattc
     9181 tgccgtttat ggcattcgtc tcggcgctca ttggcatcat tatggcttct ggccttggtg
     9241 actggattgc ccacggtctt gctccgctgg cgagccatcc actgggtctg gtcatgctgg
     9301 cgctcatctg ctccttccca ctgctttcac ctttcctcgg cccaggcgca gttatcgcac
     9361 aggttatcgg cgtattgatt ggcgtgcaga ttggtctcgg caatattccg ccgcatctgg
     9421 ctttaccggc actgtttgcc atcaacgcgc aggcggcctg cgacttcatc ccggtcggtt
     9481 tgtcgctggc ggaagcccgt caggacacgg ttcgcgtcgg tgtcccttct gtactggtga
     9541 gccgcttttt aaccggcgcg ccgactgtac tgatcgcctg gtttgtctcc ggttttatct
     9601 atcaatagag gctgaaacat gaccgttatt tatcagacca ccatcacccg tatcggcgcg
     9661 agtgccactg acgccctcag cgaccagatg ctcatcacct ttcgtgaagg cgcgccagcg
     9721 gacctcgaag agtattgctt cattcattgc cacggcgagt tgaaaggtga actccatcct
     9781 ggtttgcaat ttacgctcgg gcagcatcgc tatccggtga ccgccgttgg cagcgtggcg
     9841 gaagacaacc ttcgcgaact gggtcatgtc accctgcgct tcgatggttt aaacgaagcg
     9901 gaatttccgg gcactgtcca tgtggcaggc cctgtatccg acgatatcgc gccgggatcg
     9961 gttttgaagt ttgaatctgt taaggagtaa aaaatgaatc aggttgccgt tgtcatcggt
    10021 ggtgggcaaa cattaggcgc gttcctgtgc cacggtctgg ctgccgaggg gtatcgcgtc
    10081 gcggttgtcg atattcagag cgacaaagcc gcaaatgtgg cacaagaaat taacgccgaa
    10141 tatggtgaaa gtatggcgta cggttttggt gctgacgcca ctagcgagca aagcgttctg
    10201 gcgctctctc gtggggtaga tgaaatcttt ggtcgcgtgg atttgctggt ctatagcgcc
    10261 ggaatagcaa aagcagcctt tatcagcgac ttccagctcg gcgattttga ccgttcgcta
    10321 caagtgaatc tggtgggtta tttcctgtgt gcccgtgaat tttcgcgttt gatgatccgc
    10381 gacgggattc agggtcgcat tattcagatc aactcgaaat ccggcaaagt gggcagcaaa
    10441 cacaactctg gctacagcgc agcgaaattt ggtggcgtcg ggctgactca atcactggcg
    10501 ctggatctgg cggagtacgg cattacggtg cattcactga tgctcggtaa cctgctgaaa
    10561 tcgccgatgt tccagtcact gttgccacaa tacgcgacca agctgggtat caaaccggat
    10621 caagtcgagc agtattacat cgacaaagta ccgctcaaac gcggctgcga ttatcaggac
    10681 gtgctgaata tgctgctgtt ctacgccagt cctaaggcgt cgtactgcac cggacagtcg
    10741 atcaatgtca ccggcggtca ggtgatgttc tgattaacag cggagatccg ttaaggatct
    10801 ccgtgagact atagaatgcc tgatgcgcta cgctcatcag gcatacagga cttccgccac
    10861 tacattaagg aaaagttatg gtatccgcac tcatcaccgt cgccgttatc gcctggtgtg
    10921 cgcaactggc cttaggcggc tggcaaattt ctcgttttaa ccgtgccttc gacacactat
    10981 gccagcaagg gcgggttggc gtgggccgtt ccagcgggcg ctttaaaccg cgggtcgtgg
    11041 tcgccatcgc gctggacgat cagcagcgca tcgtcgacac cttgtttatg aaaggactga
    11101 ccgtcttcgc ccgaccgcaa aaaattcccg caattaccgg tacgcatgcg ggtgatttac
    11161 agcccgatgt gatctttccc catgatccac tatcacagaa tgctctatca ttggcgctta
    11221 aactgaaacg tggataattt cgttgtgaat gttacttgct tgcgaagtta tcattttgaa
    11281 acctaaatca ggtaatcacg cccatgaaac ctcgtcagcg tcaggccgcc attctggagt
    11341 atctgcaaaa gcagggtaaa tgctcggttg aagaattggc gcaatacttt gacaccacag
    11401 gcacaaccat tcgcaaagat ctggtcattc tggaacatgc cggaaccgtc attcgtactt
    11461 atggcggagt ggtgttgaat aaagaggaat ccgatccgcc tatcgatcat aaaacgctca
    11521 tcaacaccca caagaaagag ctgattgcag aagctgccgt tagttttatc catgatggcg
    11581 attcgatcat tcttgatgct ggcagtaccg ttttgcagat ggttcccctg ctctcgcgct
    11641 ttaataacat cacggtgatg accaacagcc tgcatatcgt caatgcgcta tccgaactgg
    11701 ataacgaaca aactatcctg atgccaggcg gaacgtttcg taaaaaatcg gcctcatttc
    11761 acggacagct ggcagagaat gccttcgaac atttcagttt cgataaattg tttatgggca
    11821 ccgacggcat cgatctcaat gcgggcgtaa ccaccttcaa cgaggtttat accgtcagta
    11881 aggcaatgtg caatgccgcg cgcgaagtga ttttgatggc ggactcatca aagtttggcc
    11941 gtaaaagccc caacgtagtt tgcagtcttg aaagcgtcga taagcttatt accgacgcag
    12001 gtatcgatcc ggcgtttcgt caggcgctgg aagagaaagg gatcgatgtg atcataaccg
    12061 gagagagcaa tgagtgaagc actactgaac gcgggacgtc agacgttaat gctggagttg
    12121 caggaagcaa gccgtttacc ggaacgtctg ggcgatgatt ttgttcgcgc cgccaatatc
    12181 atcctgcact gcgaaggcaa agtggtggtt tcgggaattg gcaaatcggg ccacattggt
    12241 aagaaaatcg ccgcaacact tgccagtacc ggcacaccgg ctttttttgt ccatccggca
    12301 gaagcgctgc acggcgatct ggggatgatt gaaagccgcg atgtgatgct gtttatctct
    12361 tactccggtg gcgcgaagga actggatctg attattccgc gtctggaaga taaatctatc
    12421 gcgctgctgg cgatgaccgg caaaccgacg tcaccgctgg gcctggcggc gaaagcggtg
    12481 ctggatatct ccgtagaacg cgaagcctgc ccgatgcacc ttgcgccgac ctccagcacc
    12541 gtcaataccc tgatgatggg tgacgcgctg gcgatggcgg tcatgcaggc gcgcggattt
    12601 aatgaagaag attttgcccg ctcccaccca gccggggcac tgggcgctcg cttgctgaat
    12661 aaagtgcatc atctgatgcg ccgtgacgat gccatcccac aggtggcgtt aaccgccagc
    12721 gtgatggatg cgatgctgga actcagccgc accggtctgg ggctggtggc ggtatgtgac
    12781 gctcaacaac aggtacaagg cgtctttacc gacggcgatt tacgtcgctg gctggttggc
    12841 ggcggcgcac tcaccacgcc agttaatgaa gcgatgacga caggcggcac cacgttgcaa
    12901 tcgcaaagtc gcgccatcga cgccaaagag atcctgatga agcgcaaaat cactgccgca
    12961 ccggtggtag atgaaaacgg caaactcacc ggcgcaatta acctgcagga tttctatcag
    13021 gccgggatta tttaatcctt cattcccaga cgtttcgcca gccgatgcag gttggcgacg
    13081 tcggtttcca gcatccgcgc gcaggcagcc cagttgtgat gattttgtgc cagcgcctga
    13141 cgaatagttt cacgctggaa cgcttctgtc gcttcacgca ggttttgctt aacaacgggc
    13201 accgccgcca cttctggcgt cggcaacgtc acctcaggaa aagcaaaatg ttgcgcctca
    13261 agaatcactt catcgccgtt gcgggtggct ctcgacagca ctaccgcccg atgaatagca
    13321 tgttccagtt cgcgcacgtt ccccggaaaa cgatagtgtt gcagtaaatt tcgcgctccg
    13381 gcacttaata ccacgcggga gagccccagc cgcaaacgac actgctcgca gaaatacccc
    13441 gccagcagaa tgacatcatc gccccgctca cgcagcggcg gcaccgaaag tggaaacacg
    13501 ctcaggcgat gaaacaagtc agcgcgaaat cgccctgcca gcacctcttc gcgtaagtcg
    13561 cggttagtcg ccgccagcac gcgcacatcg acccgcaaac tgcggtcatc gccaacgcgc
    13621 tgaatatcgc catactgcaa caccctcagc agcttggcct gcaatgccaa cgacaactcg
    13681 ccgatctcat ccagaaacag agtgccgtta tccgccattt caaacttccc gctgcggtta
    13741 ctgatagcgc cagtaaacgc tcctttcaca tgcccgaaca actcactttc cgccacactt
    13801 tccggcagtg cggcacagtt gagatagacc agcggattca ccgcccgtgg cgaggcttca
    13861 tgaatcgctt tcgccaccag ctccttaccg gttccagtct caccgctgat caggacgttg
    13921 agatcggacg ccgccacaat ctcaatctct tttttcaatt gcgtcatgcc tggcgacaag
    13981 ccaatcatct gcgtctgttt caccgcttca aacggcgcgg catcgcccgg cagcatattc
    14041 tggctttcca gttgttcaat cagcaacgca ttgcttaacg ctcccgccgc cagcgcggca
    14101 atcagccgta actcttcgtc gctgaaaaca tcgaactgat cgggctgcat cccgtcgagc
    14161 gtcagtgcgc cgatcaggtt ttgcccggca aacaatggca gaccaacgca ggcgtgaacc
    14221 ttcagactct cctgcccagg aatcaaaccg tcatagggat cgggcaattc gctgtctgca
    14281 ggaaagcgca ccacgtcccc ggcgcgggca atcgcttcaa gccgtggatg cccttccagc
    14341 gcaaagcgtc taccgagtac atcctttgcc agaccgtcga tggcaagcgg aataaactgc
    14401 cgcgaatcgt aacgtagcaa cgcagacgca tcgcactcca gcacctgacg tagcgtggtg
    14461 atcaggcgct gaaaacgatc ctgatgacca atcccacgct gcaattcgat ggcgatattc
    14521 gccagcacat caacggaaaa actcatcttt gcctcactgt caatttgact atagatattg
    14581 tcatatcgac catttgattg atagtcattt tgactactca ttaatgagca taattttatt
    14641 tatagagtaa aaacaatcag ataaaaaact ggcacgtaat ctgcaattag caagacatct
    14701 ttttagaaca cgctgaataa attgaggttg ctatgtctat tgtggtgaaa aataacattc
    14761 attgggttgg tcaacgtgac tgggaagtgc gtgattttca cggtacggaa tataaaacgc
    14821 tgcgcggcag cagttacaac agctacctca tccgcgaaga aaaaaacgtg ctgatcgaca
    14881 ccgtcgacca taaattcagc cgcgaatttg tgcagaacct gcgtaatgaa atcgatctgg
    14941 cggatatcga ttacatcgtg attaaccatg cagaagagga ccacgctggg gcgctgaccg
    15001 aactgatggc gcaaattccc gatacgccga tttactgtac tgccaacgct atcgactcga
    15061 taaatggtca tcaccatcat ccggagtgga attttaatgt ggtgaaaact ggtgacacgc
    15121 tggatatcgg caacggcaaa cagctcattt ttgtcgaaac gccaatgctg cactggccgg
    15181 acagcatgat gacttacctg acaggcgacg cggtgctgtt cagtaacgat gctttcggtc
    15241 agcactactg cgacgagcat ctgttcaacg atgaagtgga tcagacggag cttttcgagc
    15301 agtgccagcg ttactacgcc aatatcctga cgccgttcag ccgcctggta acaccgaaaa
    15361 ttaccgagat cctgggcttt aacttaccag tcgatatgat agcgacctct cacggcgtgg
    15421 tatggcgcga taacccgacg caaattgtcg agctgtacct gaagtgggcc gctgattatc
    15481 aggaagacag aatcaccatt ttctacgaca ccatgtcgaa taacacccgc atgatggctg
    15541 acgctatcgc ccagggaatt gcggaaaccg acccacgcgt ggcggtgaaa attttcaacg
    15601 tcgcccgaag cgataaaaac gaaatcctga ccaatgtctt ccgctcaaaa ggcgtgctgg
    15661 tcggcacttc gacgatgaat aacgtgatga tgccgaaaat cgccgggctg gtggaggaga
    15721 tgaccggatt acgcttccgt aacaaacgcg ccagtgcttt cggatctcac ggctggagcg
    15781 gcggtgcggt ggatcgcctt tccacgcgcc tgcaggatgc gggtttcgaa atgtcgctca
    15841 gcctgaaagc caaatggcga ccagaccagg acgctctgga gttatgccgt gaacacggtc
    15901 gcgaaatcgc ccgtcagtgg gcgctcgcgc cgctgccgca gagcacggtg aatacggtag
    15961 ttaaagaaga aacctctgcc acgacgacgg ctgacctcgg cccacgaatg cagtgcagcg
    16021 tctgccagtg gatttacgat ccggcaaaag gcgagccaat gcaggacgtt gcgccaggaa
    16081 cgccgtggag tgaagtcccg gataacttcc tctgcccgga atgctccctc ggcaaagacg
    16141 tctttgacga actggcatcg gaggcaaaat gagtaacggc attgtgatca tcggttcggg
    16201 cttcgccgcc cgccaactgg tgaaaaatat tcgcaaacag gacgccacta ttccattaac
    16261 cctgattgcc gccgacagca tggatgagta caacaaacct gacctcagcc atgttatcag
    16321 tcaggggcaa cgtgccgatg accttacccg ccagacggcg ggtgaatttg ccgagcagtt
    16381 taatctgcac ctgtttccac aaacctgggt gacggatatc gatgccgaag cccgtgtggt
    16441 gaaaagccag aataatcagt ggcaatacga caagctagta ctggcaaccg gtgccagtgc
    16501 ctttgtcccg cctgtgcctg ggcgtgagtt aatgctgacg ttaaatagtc agcaagagta
    16561 tcgcgcctgt gaaacgcaac tgcgggatgc ccgacgcgtg ttgattgttg gcggtggttt
    16621 gattggtagc gaactggcga tggatttttg tcgtgcaggc aaagcggtca cgctaatcga
    16681 caacgctgcc agtattctgg cgtcgttaat gccaccggaa gtaagcagcc gcttgcagca
    16741 tcggttgacg gagatgggcg ttcatctgct gttgaaatct cagttacagg ggctggaaaa
    16801 aacggattct ggcattctgg caacgctgga ccgccagcgt agcatcgaag tggatgcggt
    16861 aattgccgcc accggactgc gcccggaaac cgccctggca cgacgcgccg ggctgacgat
    16921 taatcgcggc gtttgcgtcg atagttatct gcaaaccagt aatgacgata tttatgcgct
    16981 gggcgattgc gcggaaatca acggtcaggt actgccattc ctgcagccga ttcaacttag
    17041 cgcgatggtg ctggcaaaaa atcttctcgg caataacacg ccgctgaaac taccggcgat
    17101 gctggtgaaa atcaaaacgc cggaattacc gctgcatctg gcaggcgaaa cccagcgtca
    17161 ggatttacgc tggcaaatta ataccgaacg ccagggaatg gtggctcgcg gtgttgacga
    17221 tgctgaccag cttcgcgcct ttgtggtcag tgaggatcgg atgaaagagg catttggatt
    17281 gttgaaaaca ttgccgatgt aggtgggcta ctgtgcctaa aatgtcggat gcgacgctgg
    17341 cgcgtcttat ccgacctacg gggacgcatg tgtaggccgg ataaggcgtt tacgccgcat
    17401 ccggcaatgg tgtccaaatg caacacgttt tatccgttct ggacttcacc cgctaaccaa
    17461 cgcgccgcag caataacccc ctgccccaga gacaaaccgc catcacccgc cggtaaactc
    17521 tgtggaaaga gcaatgtgaa atcagcgaga taatgcgcca gacgtgcacg cagcaaacgg
    17581 ttatgaataa ccccgccgct aaataccagc gtagtgatac cacgcatcgt ggcctgctca
    17641 cgcatcaacg cggcaaaacc ctgcgccagc gcatcatgaa acgcccacgc gcgttgatta
    17701 accggtgcct gccagttcag ccactgctgc cagaaagcgg cgagatccag ttgattgtcc
    17761 accagcggca tcgtcaccgg atgcgtcact ccgtggcatg aggctgcgag cgcctccaga
    17821 gcacaagccg cttcaccttc ataacttaac gtggctggcg cacagcccag tgccgccgcc
    17881 actgcatcga acaaccgccc acacgatgac gccagcggcg cgttaattcc acgctcaatg
    17941 gcccgcgcca gcacgctcca gttttgctgt tgcacacttg ctgtttcaga gtaattctgc
    18001 cactccggca caaagcgcag gcactgcgcc agcaggtttc gccacggctg cttcgctgcc
    18061 aaatcgccac ccggaagcgc caccgcaggc aagccgccca ggtgctggca ttcgcgatag
    18121 ttcacccgca ggcactcgcc gccccacaaa gcgccgttct cccccatacc gataccgtcg
    18181 agcgtcaaag caatgacatc accgccatcc agcggccact gatgctctgc cagacacgcc
    18241 gctgcgtggg catgatgatg cagcaccgtt tgcgtcggca gattcatttc gcgcgcccac
    18301 tggctagaga catagcccgg atgcgcgtca tgcacaacgt attgcggagt gaaatcgtag
    18361 atgttttgca tcaggcgtaa cgcttcgcgc cactgcatct ggatgccatc gtcacttaaa
    18421 tcgcccagat gctgactcaa caccgcttgt tcaccgcgca ccaggcagaa ggtgtttttc
    18481 agatccgcgc cgagacacag cacaggcgga acatttttaa agcccggagg caaagccagc
    18541 gcatccggca catacccccg cgaacggcgc agcatttcgc cgctttcgcg caccaccgaa
    18601 tcatccatcc gctgcacgat gtcgcggtta tgtatcaaga atccgtcggc aatgccctgc
    18661 aaatccgcca gcgcctgttc gttgctgata gccggtggtt taccgctcag gttgccggag
    18721 gtcatcacca gcgggcattg cagttcctgt aacagcaaat gctggagcgg gttcgcaggc
    18781 aacatcactc cgacttcgtt aaggccaggg gcgatatcat cacaaagctc aggtacgtgt
    18841 tttttatcca ccagcacaat cggcgcagcg ggcgtggtaa gcaactggcg cgcagcgtct
    18901 ggtaaacttt ctgctactgg caacatgact gccagcggtt tcgccgggcg gtgtttgcgc
    18961 tcccgcagtg tcgccaccgc gttactgtta cgtgcatcgc aggcaagatg aaatccgcca
    19021 atccctttga tggcgacaat tttgcccatt tttaactgtg cgatagctgc ctgtaatgcc
    19081 gcctcttgtt ccgcatgttc accatgactt acccattcaa gatgcgggcc acactccggg
    19141 caggccaccg gctgggcgtg gaagcgacga tcgagcgggt cgcggtactc tttgtcacaa
    19201 gccggacaca acggaaacgc cgccatcacg gtaaacgggc ggtcgtaagg catggcgcga
    19261 ataatggtga aacgcgggcc gcagtgggta cagttgataa acggataacg ataacgccgt
    19321 tcgcctgggg tattcatttc ggcaaggcaa gcagggcaag tagcggcatc cgggacaatt
    19381 tgcgtattca tggcacctcc cgcgctctgg cggatggtga actcagtggg cagttgtgac
    19441 cagatgtacg gctcacgctc gacgctatca atacgcgcca gcggcgggca gtgctgatgc
    19501 aattgaacaa gaaacgtttc cgggtcttcc cgcagccgga cttctacgcc atcgccgtca
    19561 ttacagacat cgccgtgaag atttaattgc tgtgccagct gccagacaaa cggacgaaaa
    19621 ccgacgccct gcactttgcc acgaatacgc agttggacac cgcaagatgt gttttttgcc
    19681 attgagttat tcccgccatc atgaattgcg taacccgccc agccggacac gacagcgtcg
    19741 catccggcag tcacaggtcg gcgataccgc ccgctccgta ttctacgaat atttccggga
    19801 attcctttga tgccagaaca gttctgtaag atttttagaa catcagcgcc gtacggcggc
    19861 gtttttctgc actcagttgt tcaagtttat tacgatcgac acaaatcagc gcatgagtcg
    19921 ggcaagccgc catacacgcc gggccgtctt cacgatggtt gcacaggtca catttattgg
    19981 cttcggcttt gtcagcccgt acattcagcc ccgcgccgct gttgcgaatc accggacgta
    20041 ccaccacttc catcgcgcca tacgggcaag ccacaacgca ggttttgcaa ccaatgcaac
    20101 gttcctgcat cacatgaaca aaccctttat cacggctgat agcaccattc gggcagacgt
    20161 tagcgcacgg tgcatcttca cactgacggc aaactgtcgc cgtggaaatg ttcacacctt
    20221 taatgacatg gatacgcggt aaaaatgttt ccggggtcag cgatgcacag tcctgatttt
    20281 cctgatgaga aaccacgcac gccacttcac aggtacggca accaatacat ttactcgcgt
    20341 cagcaatgat gaaacggttc atcaaattct ccagcaatga cagttaatgc gccgatacat
    20401 tcacaaatca tgccagtttt taattcactg ttatttaagg aaattaattt ctgtaatgca
    20461 ggaaaaaaga tgtcatcgac actagtgacg atgacatgtg atgacaatgt ttatcgcgaa
    20521 agagcaatga gtgagtcgcg gtggatcagt tttccgctga aggttttcgg cggtgagaaa
    20581 tccccgccat cgagcataaa aatcagccgt ccaataattt cctgaatcat ctcagtcacc
    20641 ggaattttta cgctggagag cgccggaacg atataggggg caattgcgat atcatcgaat
    20701 ccgataactg acacctgctc tggcaccgct acgccgcgct cgtgcaacgc tttcatcgca
    20761 cctatcgcca tatcgtcgtt actggcaact aacgcgctaa atttagcccc acgttcgagc
    20821 aacgtttcta ccccttcggc cccgctggcg ggcgtccatt taccgttagc gataagtttt
    20881 tcattgagcg caataccatg ctgcgccagc gcgtctttat acccagcaag acgttcaatg
    20941 ctggtggggg aatccatcga gccggtaagg aaagcaatct cctgatgtcc ggcgtttatc
    21001 aactctgcca cggcattaaa gctggtctgt ttatgatcgc accagacgct atggctgctg
    21061 tttttgcgca ggcggcgatt aagcaccatt atcggctgac tgtgcgcgtc aatgatgtca
    21121 tcaatctcat ccacgcttaa aaaacgcggg taaatcataa tggcgtcgca gcgcaaatcc
    21181 agcagatact gaatcgcctg gcgctcttct tcggcgctgt gtttaccatc tgccaatagc
    21241 aactgccgcc ctttctcttc cgccattcgc gcggcatgaa acagtaattc actaaaataa
    21301 atgccgtgat aaagcgtgtt ggtcactacc agccccagcg tctgagtact cttcgccgac
    21361 agattgcggg ccagcaaatt tgggcggtaa ccgctctctt ctaccgcctg aaacacgcga
    21421 tctttagtct cctggctgac gtagccatta cctgaaagca cgcgggaaac ggtcgctttt
    21481 gaaaccccgg cgcgcttcgc cacttccagc atcgtcgtca tcattttcat ccctttacac
    21541 gcaatcaacg cagtgtactg caccgtttgc cgattgtcct tgcgcaatcg gcgggaaaaa
    21601 tattcaggtg accggtttca ccaatataaa aaatgaacaa ttaactctct tgcttattta
    21661 gtgacaacta ttcatgattt tgtgaaaccg gtttcctaat tccgtttcag catcggcatt
    21721 tttccgttac gtcgactgat aacaactaca tctaccctac tgataacagg ataaaatccg
    21781 atggccaaaa attatgcggc gctggcacgc tcggtgatag cggcactggg cggcgttgat
    21841 aacatctcgg cggtcacgca ctgtatgacg cgtttgcgct ttgttatcaa agatgaccag
    21901 cttatcgaca gcccgacgtt aaaaaccatc cccggcgtgc tcggcgtggt acgtagtgac
    21961 aaccagtgtc aggtgattat cggcaatacc gtttcacaag cctttcagga agtcgtcagc
    22021 ctgctgccgg gagatatgca gcccgcacag cccgtgggta aacccaaact cacgctgcgt
    22081 cgcattggtg cggggatcct cgatgcgctg atcagcacca tgtcaccgct gatcccggcg
    22141 attatcggcg gatcgatggt caaactgctg gcgatgatcc tcgagatgag cggcgtgctg
    22201 acaaaaggat cgccgacctt aaccattctg aatgtgattg gtgacggtgc tttcttcttc
    22261 ctgccgctaa tggtcgcggc atctgccgcc atcaaattta aaaccaatat gtcgctggcg
    22321 attgccatcg cgggtgtgct ggtacatccg agttttattg aactgatggc gaaagcggcc
    22381 cagggtgaac atgtcgaatt tgccctgatc ccggtcaccg cggtgaaata cacctacacg
    22441 gtgatcccgg cgctggtgat gacctagtgc ctgtcatata tcgaacgctg ggtggatcgc
    22501 attacaccgg cggtgaccaa aaacttcctc aagccaatgc tgattgtgtt gattgccgca
    22561 ccgctggcaa tcctgctgat tggcccgatt ggtatctgga tcggtagcgc catttcggcg
    22621 ctggtttaca ccattcatgg ttatctgggc tggctttcag tcgccattat gggcgcgctg
    22681 tggcctctgc tggtaatgac cgggatgcac cgcgtcttta cgccaaccat cattcagacc
    22741 attgccgaaa ccggcaaaga agggatggtc atgccgtcag agatcggcgc taacctgtcg
    22801 ctgggcggtt catcactggc ggtggcgtgg aaaacgaaaa acccggaact gcgccagacg
    22861 gcgctggcag cggcggcatc agccattatg gcggggattt ccgaaccggc gttatacggc
    22921 gtggcgatcc gcctgaaacg tccgcttatc gccagtctta tcagcggttt tatttgcggc
    22981 gcggttgccg gtatggcggg gcttgccagc cactcaatgg cagcgccggg gttatttacc
    23041 agcgtgcaat tcttcgatcc ggcgaatcca atgagcatcg tctgggtgtt cgcggtcatg
    23101 gcgctggcgg tggtgctgtc gtttatcctc acactgttgc tcggctttga ggatattcct
    23161 gttgaggaag cggctgccga ggcgcgaaag catcagagcg cacaaccgac cgtcgccaaa
    23221 gaagtaagtc ttaattgagg atgaaaatgt cagtatttcc gcaaggtttt ttatggggcg
    23281 gcgcgcttgc cgccaaccag tctgaaggtg cgttccgtga aggtggcaaa ggactgacca
    23341 cggtcgatat gatcccgcac ggcgagcatc gaatggcggt gaaactgggg ctggaaaaac
    23401 gttttcagtt gcgcgatgac gagttttatc ccagccatga ggcaacggat ttttatcatc
    23461 gttataaaga agatatcgcc ctgatggcag agatgggatt caaagtgttc cgtacctcaa
    23521 ttgcctggag ccgcctcttc ccgcagggcg atgaactgac gcccaaccag cagggcattg
    23581 ctttttaccg ttctgtcttt gaagagtgta aaaagtacgg tatcgaaccg ctggtcacgt
    23641 tgtgccactt cgatgtgccg atgcatctgg tcaccgaata tggctcctgg cgtaaccgca
    23701 agctggtgga gtttttcagc cgctacgcca gaacctgctt tgaagcattt gatggtctgg
    23761 tgaaatactg gctaaccttc aatgaaatca acattatgtt gcatagcccg ttctccggcg
    23821 cgggtctggt gtttgaagaa ggtgaaaatc aagatcaggt gaaatatcag gccgcgcatc
    23881 accagctggt tgccagtgcg ctagccacca aaatcgccca tgaggttaac ccgcaaaatc
    23941 aggtgggctg tatgctggcg ggcggcaact tctaccctta cagttgcaag ccggaagatg
    24001 tctgggcggc gctggagaaa gatcgggaaa acctgttttt tatcgatgtg caggcgcggg
    24061 gcgcgtatcc ggcttactct gcccgcatat ttcgcgaaaa aggggtaacc atagataaag
    24121 cgctgggcga tgatgagatc ctgaaaaaca ccgtcgattt tgtctctttc agctattacg
    24181 cctcgcgctg cgcctcggcg gagatgaacg ccaacaacag cagtgcggcg aacgtggtga
    24241 aatcgctgcg taacccgtat ctgcaagtga gcgactgggg ctggggaatt gatccactcg
    24301 gtctgcgtat caccatgaac atgatgtacg accgctatca gaagccgctg tttctggtgg
    24361 aaaacggcct gggcgcaaaa gatgaacttg ctgccaatgg cgagattaac gacgactatc
    24421 gcatcagcta tttacgcgaa catatccgcg caatgggcga agcgattgca gacggcattc
    24481 cgctgatggg ctacaccacc tggggctgta ttgatttggt ttccgcctca acgggtgaaa
    24541 tgagcaaacg ctacggtttt gtctttgttg accgtgacga cgcaggcaac ggcacgctga
    24601 cgcgcacacg taagaaatcg ttctggtggt ataaaaaagt gattgccagt aatggtgaag
    24661 atttagagta agcaacagtg ccggatgcgg cgtgaacgcc ttatccggcc tacatgtcgc
    24721 acgaaccagg aaggcccgat aagcgcagcg catcgggcaa tctggggttt ggcatcagtc
    24781 aaaatcaaag aaaccattcc ggcttaacgc caaatcgggc tgaaagtgcc ttaatgtggg
    24841 atatcgtcag ggatcgttgt ccagacaaaa tctgactgac cagggattta gagccgattt
    24901 cgttcttcaa atccgcatag gtcagattat gttgatcaat cagcgtgcga agtaacgcca
    24961 cgccaacagg cattgccgca acggctttgt cgaactctgc aaactcttgg ttgttgtttt
    25021 catattctgc aatgcggctc gccagaaagt cgataagtgg attggtgtca tcttcttcaa
    25081 tcaaatattc gaccagttcc agtgcttcac gataatcatc ttctgagtca ctacctccca
    25141 gaaaggggac tgcattgacc agttctcttg ttgctttaac agcgcgtgca gcattagcag
    25201 tcattctttg ttctctctgt agtaacgtgt cagtttgtca tactcggcgt gattcgttat
    25261 gtgcttcaca aagaaccgct tattaacaaa gtttatgtag gcaatcaccc ttaggttgtt
    25321 cccacctaca tccagaacgt accatttgtt gcgatattta aaattgtcga ggttgggaaa
    25381 cgccgttcgc atctcttctg gcgtagaaaa atccgtttcc ttaataaccc gatacaatgc
    25441 ctgaagagcc agagcgtcgt taggatattt acgcgcagac tcttcaaatg gcgctttaga
    25501 aatgatgtgc atttttaagt tttaacttcc ttatagtaag taaacagcca catctgcgag
    25561 ttctatcctt atgtgttcgg aaactatagc tcatcaaatc caagttcaca atatgtaaac
    25621 acttgtgcgc gatataatta cccccaaaat cctcatagcc actctgcaaa aagtcagttc
    25681 tggttcaccc ctcaagcaca tcccccttta tcaagcaacg ctgcgtctgc gccagatctt
    25741 ccgtttgcgg gtcgcgcccg gcgatggggt tgaggacgag tttacggttg ggggtataag
    25801 taaaacgcgt gagcgcggga tagcttaact tgccaaacca gccgtgaaca cgctgccatt
    25861 tggcgtcttt tacctgatac agactggtta tccagtaacc agaattaaac tgcatatcca
    25921 gcaattgcgt gtgaccattg ccttgcaaat ccagcaaatc atctacgcca gttttgcttg
    25981 cggtgtagaa tcccctgggt tcaaagacac agggacggcc ttcgcgcgtg aacgtcataa
    26041 gaattaaatg tgcataagga gccagaccat taccggtgct tggtaaccag ataaccagat
    26101 cctgaatgcc gttacgatca agatcggcgc gatagagttg ccctttgccc gctatgtttg
    26161 ccagcatacc cacaggcaca gaccaggatt taccggcgcg atctttaccg ttcaattgcc
    26221 attcactatc atcatttggc atcttttcaa gcgtcacgcc agcaacgccc acttttctaa
    26281 aatgccaggt ttgctcggca tcataaggca tggtcaggag aggtttcagc gcccgctttt
    26341 gcgcgtattc gctgtcactc attggcagat attcggtaaa gctggtggcc tgtgcaggca
    26401 gcactaagct caataaaaaa gcgcacatcc ctgttctgat attcatttcg ctgaattccc
    26461 tgatggagaa ttattcttct tccaccgcta actgcgcgaa gccgccattc ccttcccagc
    26521 cttccagtcg ttgataaacg gtttctaccg catctttaat cggctgagtc atcgggtagt
    26581 aaaagccgac gatatccggc tggatgccga ggaaaatcac ttcgccaatg tcttctttca
    26641 actggtcgat aagatagttc agcggcatgt tatgggtagt catcataaac atctcggcga
    26701 tatcatccgg gtcgatgatg cggatttcgc cggggtttag ccccatatca gtagcgtcaa
    26761 caatcagtaa ccgcgtaggg cgcagttcac ggatggcgac gatgtcgttt tccggtgcac
    26821 taccgccatc aatcaccacc cagttacctt tcggcgcggc ggcgcacttt tccgccagca
    26881 gcgggccagc gccatcatcg cccatcatgc tattgccaac acagagtaaa acgtcagtca
    26941 cgcagtctcc tcaccatcaa atagatggcg ttttcctgat gaatatcatg cagcatactt
    27001 aacagcattg tactccattc cttctgctgc gaagtttgcg ctgcccgggc tttatcgaat
    27061 gcgttggcca gcatcggcac atggttaatg tcgatgacga tctcgccata tttcggtacg
    27121 ccttccattt tgcggcgggc ttcgctgccc gcctccagag tggcaatcca tgccagatat
    27181 tcatcccatg ggcaggtgag cgccgcttcc aggcaatcga taaccccaag gtggtgacca
    27241 atcgccaggc tgtaatagac cacctgctgc gcctcggcgg gcgtggcatc gttctcatca
    27301 ataaatttac ggctcagttg actgaacacc accttttcac tcatcggata cgcgcctctt
    27361 caacaacatg attcagatgg ctgacaatct cgttcagacg cggatcgttt tccgcttcca
    27421 gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt aaggtaatca tcggcaatct
    27481 gacgaccgta acgataaccc gccaggcgac gtgcttcgcg atccactttc acgcgcagcg
    27541 gctgcaccat atcgccatgc agaatctcag ccggttgttc atcctgctca cccgggccac
    27601 gggcgtgaat tttctgctca agcaggccga gtgccattgc aaagccgtac agcgtggcgg
    27661 caggcgttgg cgggcaaccc ggaatgtaga catctaccgg gacgatttta tccgtaccgc
    27721 cccacacgca gtagagattg tggaaaatac caccgctgtt accgcaggca ccgtaggaga
    27781 tacaaatttt cgggtccggc gcggactgcc acgcacgcag cgcaggggat cgcattgcac
    27841 gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg cggtgaagga acgactttaa
    27901 tgccgaagcg ttctgcatca aacagcggcg ataaagtggc gaaaatttcg atttcgcagc
    27961 cgttgcagcc gccgcagtcc acgcggtaaa cataggcaga acgtttgatt tttttcagta
    28021 acgacgcctt catgctggcg atggattcat ccaccgtcat cgggaccgga atgccgttgg
    28081 cgtcacgggg gcctaataaa ttgctcatca gatggcctct ttcatatggc gagtcagttc
    28141 aatacggtcg gacggcacca ggcatttctg gcgcttacat tccgggcaag tctcaaagct
    28201 ttcgcggtgg ttttccgcgc ggctgtcacc attgtgctta agcagcgcaa tggcgtagtc
    28261 gatctctttc tggacggcga aaggacgatt gcagacgcga cagctgcaca gcacaaaacg
    28321 ggactgttgc aggaaatctt ctttcttcca caccgccagt tcgtactctt gcgacagttt
    28381 gatcgctgcc gtcgggcaga cttcttcgca gcgtccacag aagatgcagc gcccgagatt
    28441 aaactcccac gccagctcac cggtggcgag gtcagtttca accgttaagg cgtttgacgg
    28501 gcaggcattg acgcaggccg cacagccgat gcactgctgc gggttctgct ccggcttacc
    28561 acggaagttt ttatcaaccg caatcggctc cagcggataa gacgaggtcg ccgtgccggt
    28621 tttgatgact tttttgataa aggtaaacat ggcgattcct tatttcagcg gcgagttttt
    28681 acgctcaatg ctgtaacgct cgagttcttt gtacggcacc actttgctct ttttcttacg
    28741 cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa gggtccaggc taccgataat
    28801 cagcggcgca tcggaaacgg tgttaccgcg cagcatgtag cgcagggtcg gccagttcgc
    28861 gtaggtcgcg gcacggcagc gccagcggta cagcttctgg ttgtcgccgg tcatgctcca
    28921 gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc gcgaaacggt gcggaatgta
    28981 ggtaaagcct tccaccatca gcggaccgcc cggcaggtta tccagaccgt agtcgatcat
    29041 gttcagcgcg gtataaactt cgttgatacg cactttcaga cgggaaataa cgtcgcaacc
    29101 ctgctcgctg tggacttcca ttggcagcag gccataaccg acaaacgggt gatcggcgcg
    29161 agtatcgcgg gcgtgaccgc tggcgcgaac catcgggcca acgttactga aatcgcgggc
    29221 gatttccggg tccagacgac caatgccgac ggtgcgctgt tccatgttcg gcgtactcag
    29281 cagcacatcc accagctcct gcacttcacg acgcatctgt tgcgccagct ggcgggtctg
    29341 gatcatgtca tctttcaaca gatcgcgacg aatcccgccg atcaggttca ggccgtaggt
    29401 tttacgcgca ccggtgagga tctctgccat tttcatggag gtttcacgca cgcggaagaa
    29461 ctgcataaag ccggagtcaa agccggtaaa gtggcaggcg agaccgaggt tgagcagatg
    29521 cgagtgcagg cgttccacct ccagcagaat ggcgcggatc atctgcgcac gttctggcac
    29581 ctgaataccc atcgcgtttt ctaccgacgt ggtgtaggcg gtgctgtggg caaagccgca
    29641 gatcccgcac acacggtcag agaggaaggt cacttcgttg taacccatac gggtttccgc
    29701 cagtttttcc atgccgcgat gaacgtagaa cagacggtag tcggcgtcga taatgttttc
    29761 gccatcgacg aacagacgga agtggcccgg ttcgtcagaa gtgacgtgca gcggaccaat
    29821 cggcacgacg ttgtttttct tgtcgcccag ttcgttgatg aactcgtagg tttcagcatc
    29881 ggtggtcggc gccggacgct gacgataatc catgctgtct ttacgcagcg gataaagttc
    29941 atccggccag tcatccggca gcaccagacg gcgttcatcc ggcagaccaa ccggaatcaa
    30001 accgtacata tcgcgcactt cgcgctcgcc ccacacagcc gccggaacgc gcggcgtcac
    30061 ggacggatac tccggtttgt tggcgtcaac ttcgacgcga accgttaccc aacacttagt
    30121 gcccttctcc atcgacagca cgtaataaac ggcgtaatga ccattcagtt tgcgttcgtc
    30181 gttaccaaac agcaccgaca gccagccacc ctgtttgtag taaagaaact ccaccacttc
    30241 cggcaggtag ttcaccttta cggtgacagt cagctgatct ttggtctgcc aggcgtggtc
    30301 cagcacgacg cccggaaatg cctcattcag cgcggcgaga taatgttgac ctaatttttc
    30361 ttcagacatg ctcaaactct ctttaatcac gccgccagca aggagacgaa cgctaaaaat
    30421 gcaaagccaa acccagccca ggtaatgcgc ggagtaatat caagacgcag acgggccatg
    30481 ctgttttcga acagcgcgat aaccaggacg ccgaccacca gtttgacgat ggcaatcacc
    30541 agcgccagca gcagtccacc ggcggtgaag gtttccattt gtccccacgg aataaacacc
    30601 ccgacgaaca tctgcaacac caccagctgt ttcaggctga taccccattt catgacgcca
    30661 aagccgctac cgctgtattc ggagagcgga ccttcctgca actcctgctc ggcttccgcc
    30721 aggtcgaacg gcagtttgcc catttcgata aaagtggcga acgcacaggc acaaagcgcc
    30781 agcaccagcg ggatgctctg gctcagcggc cagtgataaa cggtgtcggt gatgttgctg
    30841 atgttggtgg aaccggcaac ctgtgcggca acccacagac caagcagcag catcggttcg
    30901 accagcacgc caagcatcgc ttcacggctc gcgccgatag cagtaaacgg gctaccggta
    30961 tccagaccgg aaatggcaaa gaagaaacgc gcgatggcaa agagatacag taaggtgatc
    31021 aaatcgccca gttgcggcag cggagaaccg acggtcacca ccggcagcgc agtagcgata
    31081 gtcagcatga cgcccaccat cacatacggc gtcaggcgga acacccagcc ggaggcatcc
    31141 gggccaacgc tctggcggcc cagcagtttg ataatgtcac gatactcctg caacacaccc
    31201 ggcccacgac ggttatgtaa gcgggctcgc gccacgcggg taataccgga gagcagcggc
    31261 gcaacggcaa atagcaccag cgcctgaatt aacggatata aaacactcat tctcaggctc
    31321 ctcgtgaaac aataatcacc accagcaccg ccagttcaac cagcgccatc cggcggaaca
    31381 gcaacgcact cccctcgcac tgccagcccg gcaccagaga caccggattc agccatttgc
    31441 gtagtttcag caccggcgca aacgcctgtt tcaccggcat ggcaaaaccg tgagcggtaa
    31501 tcaccattga tttttcgtgg tcgtagccgc acacccaggc cgagccgcgg gaacgcgacg
    31561 gcaaacgatc gcctttgcaa atcgccataa tgatgaatgg cagcagcggg caggcaatca
    31621 gcagcaatgt gatcatgggt tgagaaacgg tggtgttagc aggctccaga ggcagaggta
    31681 cagcatcaga gagcatcggc agcagccacg gcgcagcaac accgccaatt acgcagcaaa
    31741 tcgccagtgc cactacgctt acgctcatca ggagcggcgc acaggtggcg ttttcggctt
    31801 ctttggtacg cggcgcgccg aggaacgtga cgccatagac tttcgccata cacatcaccg
    31861 ccagcgcacc ggtaattgcc agacccacag cgagcagcgg ccccagcaga cgggcaacaa
    31921 acgcgccact attgctcagt ttgaagaagg attgatagat aacccattcc ccggcaaacc
    31981 cgttcagcgg cggcagcgca gccattgcca ttaacccgac taacatggcg atggagataa
    32041 ccggcatttt cttgccaata ccaccgagtt tctcgatatc gcgatgaccg gtacggaacc
    32101 agacgctccc cgccccgagg aacagtacgc ttttgaacag gctatggtta agcagatggt
    32161 acagaccacc gaccaggcca agagcaatca gcgccggttg ttcgagcgcg atacccgtta
    32221 cgccagcgcc cagccccagc aggataatgc cgatattttc cagggtgtgg taagccagca
    32281 ggcgctggat gttgtgttcc atcaacgcat acagaccacc gacgaacgcg gtgatcatgc
    32341 cgagcaccag cagcgcgatc ccccaccaca gcggcgcgtt accacccagc agcgacaggg
    32401 ttaaaatgcc cagcaggcca attttcatga ctaccgtaga aaacaacgcg gcagctggcg
    32461 cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag cggaataatc ccggccagca
    32521 ggccaaagcc aatcacaccg agcagccaga tatcggaacc gagcggcagc tgttgcatac
    32581 gcatatccag caggcgcaga tccagcgtgc cataacgctg ccacagcagc cagcaagcaa
    32641 tcgccagcag cagagtgcca agacgcccca gcgcaaacca cagtttgccc tctttgctgt
    32701 tgctggtaag gaacaccgca cacaaggcca tgacttcagc cattaccacg aacatgccga
    32761 ggttgctggc gatgacggcg cagacggcgg cagccatcaa catattgatc tgcaagccgt
    32821 tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta gaggctgaca aacagaccgc
    32881 acagaccgag cgtaatcagc caaatcgcgt taagcggaga gatttgcaca tcgtagctta
    32941 ccagcgacag cgcaccgctc acgccaaccg cgccagtcag tacagtgaag cccgcggctg
    33001 ccgtatacag actaccaacc gcgccgccaa ttccggctat ccagccactt agcgtctttt
    33061 gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa ccatgccacg ccgctattga
    33121 tcagggaaat tgcgctcatt tagcctctcc actttgagcc tgctgaaaca aggtgagatc
    33181 gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg ctgacgcggg cgatatcggt
    33241 gttatccacc agatgcaggg ctttagtcgg gcacatccgc acgcaggccg gaccttgttc
    33301 atcaaaactg caaaggtcac atttgacggc gatcgcgcga atacccggca cccagtcaag
    33361 cagcgtactg atacgcgccg gagcaggcgg tgccggtggc gctttcgggg tattggcatt
    33421 tgcaggaata tccagcggac ggctgccgga aaattcaatt gcgccaaacg ggcaggcgat
    33481 gccgcacagc ttgcagctta cgcacaggct ttcattcaac tgcacggccc catcaacgcg
    33541 ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca tcttcacagt ggtgacagag
    33601 ctgcggcgca gattcttttt catttagcat cactctcagg cgcggcattg attgcaggcc
    33661 gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg tggcagccga tacagagcgt
    33721 ggagtcagca attacaaaac gattcaccag gcattcctca ggtgattgtc atttttgacg
    33781 aaaacatgcc gatgaaatgt cattttcgac actcatcgac acgcccatcc ccaaacaggc
    33841 gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc cgggctttgt ccctttacca
    33901 gttggcttaa attcaccggc acattgtttt caatggcggt gtataagctg tcgtaaacgc
    33961 cagcgatttt ttcgagatag tgttccagaa cctgttcgcg atcgcggatg ttgaccttgc
    34021 catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat ccgctgtttg tcttcgccat
    34081 cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa gccatccgcc agggtgacgt
    34141 aaatacgaaa atgttcaaaa aggacgaaac agagttcttt gtccggcgcg cagctcatgg
    34201 cgtgatgcag gcgcgctttc gataacgtga tcccctgaac cagcgaattg cagtagatgt
    34261 gccactggtc ctgtaggcga cgatgccgct gtgcgatgta atcggctttc tcgcttattt
    34321 cccaaatagt cattgtcagg ttacccgttt aacagagatg ccagctttaa gcattttttg
    34381 cgccaacttt taatttattg ttattaaaga gatttttaag ctaaagatga atttcgtcgc
    34441 cgtgtcgacg tgtcatttcg acatcatcga cattattcac cgcagggata atcaacactg
    34501 gcacaattat tgcttgtagc tggcaatagt taatgggagg cgatatgcac gaaataaccc
    34561 tctgccaacg ggcactggaa ttgatcgaac agcaggccgc aaaacacggc gcaaaacgcg
    34621 taactggggt ctggctcaaa attggcgcat tttcttgtgt cgaaaccagc tctcttgcct
    34681 tttgttttga tctggtttgc cgcggcagcg tggcggaagg ttgtaaactg cacctcgaag
    34741 aacaagaggc cgaatgctgg tgtgaaacat gccaacagta tgtgacgcta ctgacccagc
    34801 gcgtccgccg ctgtccacag tgtcatggtg acatgctaca gattgtggca gacgacggtt
    34861 tacagattcg gcggatagaa atagaccagg agtgagcgat gtgtacaaca tgcggttgcg
    34921 gtgaaggcaa cctgtatatc gagggtgatg aacataaccc tcattccgcg tttcgtagcg
    34981 cgccatttgc cccggcggca cgcccgaaga tgaaaatcac cggcattaaa gcgcctgaat
    35041 ttacccccag ccagactgaa gaaggcgacc tgcattacgg tcatggcgaa gcgggcactc
    35101 acgcaccggg catgagccag cgtcggatgc tggaagtcga aattgacgtg ctggacaaaa
    35161 ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc ccgcaagcaa ctggtgctca
    35221 acctggtttc cagccctggt tccggtaaaa ccaccctgct gacggaaacc ctaatgcgcc
    35281 tgaaagacag cgttccgtgc gcggttattg aaggcgacca gcaaaccgtg aacgatgccg
    35341 cacgcattcg cgctaccggt acaccagcga ttcaggtgaa caccggtaaa ggctgccatc
    35401 ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc actggacgat aacggtattc
    35461 tgtttatcga aaacgttggc aacctcgtat gcccggccag cttcgatctc ggtgaaaaac
    35521 acaaagtggc ggtgctttcc gttaccgaag gtgaagacaa accgctgaaa tatccgcata
    35581 tgtttgccgc cgcctcgctg atgctgctca acaaagttga cctgttgccg tatctcaact
    35641 ttgacgttga gaagtgcctc gcctgcgccc gcgaagtcaa tccagaaatt gaaatcatcc
    35701 ttatttccgc caccagcggt gaagggatgg accagtggct gaactggctg gagacacagc
    35761 gatgtgcata ggcgttcccg gccagatccg caccattgac ggcaaccagg caaaagtcga
    35821 cgtctgcggc attcagcgcg atgtagattt aacgttagtc ggcagctgcg atgaaaacgg
    35881 tcagccgcgc gtgggccagt gggtactggt acacgttggc tttgccatga gcgtaattaa
    35941 tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac atgtttgacg ttgagccgga
    36001 tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt ttgttgatga atatcgcgcg
    36061 ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac gtgcttcaca tctctcttac
    36121 accgccgaac gccctctgcg gattatggaa gtgtgcggtg gtcataccca cgctattttt
    36181 aaattcggcc tcgaccagtt actgcctgaa aacgttgagt ttatccacgg tccgggttgc
    36241 ccggtgtgcg tactgccgat gggtagaatc gacacctgcg tggagattgc cagccatccg
    36301 gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac cagggaaaca gggatcgctg
    36361 ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt actcgccgat ggatgcgttg
    36421 aaactggcgc aggagaatcc aacccgcaaa gtggtgttct tcggcttagg ttttgaaacc
    36481 accatgccga ccaccgccat cactctgcaa caggcgaaag cgcgtgatgt gcagaatttt
    36541 tacttcttct gccagcacat tacgcttatc ccgacgctgc gcagtttgct ggaacagccg
    36601 gataacggta tcgacgcgtt cctcgcgccg ggccacgtta gtatggttat cggcactgat
    36661 gcctataatt ttatcgccag cgattttcat cgtccgctgg tggtcgctgg tttcgaaccc
    36721 cttgatctac tgcaaggcgt ggtcatgctg gtggagcaga aaatagcggc ccacagcaag
    36781 gtagagaatc agtatcgtcg ggtggtaccg gatgccggta acctgctggc gcaacaggcg
    36841 attgccgatg tgttctgtgt caacggcgac agcgaatggc gcggcttagg cgtgattgaa
    36901 tcttctggcg tgcacctgac gccggattat caacgattcg atgccgaagc acatttccgc
    36961 ccggcaccgc agcaggtctg cgatgacccg cgcgcgcgtt gtggtgaggt attaacgggc
    37021 aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct gtaatcctca aaccgcattt
    37081 ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt ggtatcagta tcgtcagcag
    37141 gagagtgaag cgtgaataat atccaactcg cccacggcag cggcggccag gcgatgcagc
    37201 aattaatcaa cagcctgttt atagaagcct ttgccaaccc gtggctggca gagcaggaag
    37261 accaggcacg tcttgatctg gcgcagctgg tagcagaagg cgatcgtctg gcgttctcca
    37321 ccgacagtta cgtcattgac ccgctgttct ttcctggcgg taatatcggc aagctggcga
    37381 tttgcggcac cgcgaatgac gttgcggtca gtggcgctat tccgcgctat ctctcctgtg
    37441 gctttatcct cgaagaagga ttgccgatgg agacactgaa agccgtagtg accagcatgg
    37501 cagaaaccgc ccgcacggca ggcattgcca tcgttactgg cgatactaaa gtggtgcagc
    37561 gcggcgcggc agataaactg tttatcaaca ccgctggcat gggcgcaatt ccggcgaata
    37621 ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt gctggttagc ggtacactcg
    37681 gcgaccacgg ggcgactatc cttaacctgc gtgagcagct ggggctggat ggcgaactgg
    37741 tcagcgactg cgcggtgctg acgccgctta ttcagacgct gcgtaacatt cccggcgtga
    37801 aagcgctgcg tgatgccacc cgtggtggtg taaacgcggt ggttcatgag ttcgcggcag
    37861 cctgcggttg cggtattgaa ctcactgaag cggcactgcc ggttaaacct gccgtgcgcg
    37921 gcgtttgcga actgctggga ctggacgccc ttaactttgc caatgaaggc aaactggtga
    37981 tcgccgttga acgcaacgcg gcagagcaag tgctggcagc gttacattcc catccactgg
    38041 ggaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa aggtgttcgt cttgccggtc
    38101 tgtatggcgt gaaacgaacc ctcgatttac cacacgccga accgcttccg cgtatatgct
    38161 aataaaattc taaatctcct atagttagtc aatgaccttt tgcaccgctt tgcggtgctt
    38221 tcctggaaga acaaaatgtc atatacaccg atgagtgatc tcggacagca agggttgttc
    38281 gacatcactc ggacactatt gcagcagccc gatctggcct cgctgtgtga ggctctttcg
    38341 caactggtaa agcgttctgc gctcgccgac aatgcggcta ttgtgttgtg gcaagcgcag
    38401 actcaacgtg cgtcttatta cgcgtcgcgt gaaaaagaca cccccattaa atatgaagac
    38461 gaaactgttc tggcacacgg tccggtacgc agcattttgt cgcgccctga tacgctgcat
    38521 tgcagttacg aagaattttg tgaaacctgg ccgcagctgg ccgcaggtgg gctatacccc
    38581 aaatttggtc actattgcct gatgccactg gcggcggaag ggcatatttt tggtggctgt
    38641 gaatttattc gttatgacga tcgtccctgg agcgaaaaag agttcaatcg tctgcaaaca
    38701 tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga gccgcgtcgt taacaatgtc
    38761 gactatgagt tgttatgccg ggaacgcgat aacttccgca tcctggtcgc catcaccaac
    38821 gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg aagtcgccaa agaaatccat
    38881 tactatttcg acattgacga tatcagcatt gtcttacgca gccaccgtaa aaacaaactc
    38941 aacatctact ccactcacta tcttgataaa caacatcccg cccacgaaca gagcgaagtc
    39001 gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta aagagatgct gctgatcaat
    39061 ctccacgagc gggacgattt agccccctat gaacgcatgt tgttcgacac ctggggcaac
    39121 cagattcaaa ccttgtgcct gttaccgctg atgtctggcg acaccatgct gggcgtgctg
    39181 aaactggcgc aatgcgaaga gaaagtgttt accactacca atctgaattt gctgcgccag
    39241 attgccgaac gtgtggcaat cgctgtcgat aacgccctcg cctatcagga aatccatcgt
    39301 ctgaaagaac ggctggttga tgaaaacctc gccctgaccg agcagctcaa caatgttgat
    39361 agtgaatttg gcgagattat tggccgcagc gaagccatgt acagcgtgct taaacaagtt
    39421 gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg gtgaaactgg cacgggtaaa
    39481 gagctgattg cccgcgcgat ccataatctc agtgggcgta ataatcgccg catggtcaaa
    39541 atgaactgcg cggcgatgcc tgccggatta ctggaaagcg atctgtttgg tcatgagcgt
    39601 ggggctttta ccggtgccag cgcccagcgc attggtcgtt ttgaactggc ggataaaagc
    39661 tccctgttcc tcgacgaagt gggcgatatg ccactggagt tacagccgaa gttgctgcgt
    39721 gtattgcagg aacaggagtt tgaacgtctc ggcagcaaca aaatcattca gacggacgta
    39781 cgtttaattg ccgcgactaa ccgcgatctg aaaaaaatgg tcgccgaccg agagttccgt
    39841 agcgatctct attaccgcct gaacgtattc ccgattcacc tgccgccact acgcgagcgt
    39901 ccggaagata ttccgctgct ggcgaaagcc tttaccttca aaattgcccg tcgtctgggg
    39961 cgcaatatcg acagcattcc tgccgagacg ctgcgcacct tgagcaacat ggagtggccg
    40021 ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag tattgctaac acgcggcaac
    40081 gtgctgcagc tgtcattgcc agatattgct ttaccggagc ctgaaacgcc gcctgccgca
    40141 acggttgtcg cccaggaggg cgaagatgaa tatcagttga ttgtgcgcgt gctgaaagaa
    40201 accaacggcg tggttgccgg gcctaaaggc gctgcgcaac gtctggggct gaaacgcacg
    40261 accctgctgt cacggatgaa gcggctggga attgataaat cggcattgat ttaactgcaa
    40321 attgccggac agatctgcct gtccggcata ctattcacga ggttttttcg gacgatattt
    40381 ttccggcagt tctggcaccg gacgcttgtc atcgatgaga tgacgcacgg ttaagatcgg
    40441 atgacgccac agcattctcg gcccggccca acgcataatc tgtttcatct cttcacgctt
    40501 tgcaggctgg taacagtgca ccggacactg cttacaggct ggtttctctt cgccgaacac
    40561 acatttatcc agccgctttt gcgcgtaaac aaacaacgcc tcgtaatgct ccggctccgc
    40621 tgacgcctgc gggcatttcg cttgataaag atcgatcatt tttttaatcg tcagtttttc
    40681 acgagagata cgtttgccgg acatgctgcc tccacctcat taagatgtat ttatattaca
    40741 tcttaatctt aaagggcact atgactctaa agaagaaagg ttcgccaacc gacgcaattt
    40801 tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa cagcatcttt cccggaacca
    40861 gcatcaagaa ctcaccgttc gcttcttccc ctgaaatgat taactccggt atcatgtgcg
    40921 ccttatgtga ttacaacgaa aataaaaacc atcacacccc atttaatatc agggaaccgg
    40981 acataacccc atgagtgcaa tagaaaattt cgacgcccat acgcccatga tgcagcagta
    41041 tctcaggctg aaagcccagc atcccgagat cctgctgttt taccggatgg gtgattttta
    41101 tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg ctggatattt cactgaccaa
    41161 acgcggtgct tcggcgggag agccgatccc gatggcgggg attccctacc atgcggtgga
    41221 aaactatctc gccaaactgg tgaatcaggg agagtccgtt gccatctgcg aacaaattgg
    41281 cgatccggcg accagcaaag gtccggttga gcgcaaagtt gtgcgtatcg ttacgccagg
    41341 caccatcagc gatgaagccc tgttacagga gcgtcaggac aacctgctgg cggctatctg
    41401 gcaggacagc aaaggtttcg gctacgcgac gctggatatc agctccgggc gttttcgcct
    41461 gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg caacgcacta atccggcgga
    41521 actgctgtat gcagaagatt tcgccgagat gtcgctgatt gaaggccgtc gcggcctgcg
    41581 ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc cagcagttga atctgcaatt
    41641 tggcacccgc gatctggtcg gttttggcgt cgagaacgcg ccgcgcggac tttgtgctgc
    41701 cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg accctgccgc atattcgttc
    41761 tatcaccatg gaacgtgagc aggacagcat cattatggat gccgcgacac gtcgtaacct
    41821 ggaaattact cagaacctgg cgggcggtgc ggaaaatacg ctggcttctg tgctcgactg
    41881 caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg ctgcatatgc cagtgcgcga
    41941 tacccgcgtg ttgcttgagc gccagcaaac tattggcgca ttgcaggatt tcaccgccga
    42001 gttgcagccg gtactgcgtc aggtcggcga cctggaacgt attctggcac gtctggcttt
    42061 acgaactgct cgcccacgcg atctggcccg tatgcgccac gctttccagc aactgccgga
    42121 gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta caggcgctac gtgagaagat
    42181 gggcgagttt gccgagctgc gcgatctgct ggagcgagca atcatcgaca caccgccggt
    42241 gctggtacgc gacggtggtg ttatcgcatc gggctataac gaagagctgg atgagtggcg
    42301 cgcgctggct gacggcgcga ccgattatct ggagcgtctg gaagtccgcg agcgtgaacg
    42361 taccggcctg gacacgctga aagttggctt taatgcggtg cacggctact acattcaaat
    42421 cagccgtggg caaagccatc tggcacccat caactacatg cgtcgccaga cgctgaaaaa
    42481 cgccgagcgc tacatcattc cagagctaaa agagtacgaa gataaagttc tcacctcaaa
    42541 aggcaaagca ctggcactgg aaaaacagct ttatgaagag ctgttcgacc tgctgttgcc
    42601 gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg gaactcgacg tgctggttaa
    42661 cctggcggaa cgggcctata ccctgaacta cacctgcccg accttcattg ataaaccggg
    42721 cattcgcatt accgaaggtc gccatccggt agttgaacaa gtactgaatg agccatttat
    42781 cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg attattaccg gcccgaacat
    42841 gggcggtaaa agtacctata tgcgccagac cgcactgatt gcgctgatgg cctacatcgg
    42901 cagctatgta ccggcacaaa aagtcgagat tggacctatc gatcgcatct ttacccgcgt
    42961 aggcgcggca gatgacctgg cttccgggcg ctcaaccttt atggtggaga tgactgaaac
    43021 cgccaatatt ttacataacg ccaccgaata cagtctggtg ttaatggatg agatcgggcg
    43081 tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg tgcgcagaaa atctggcgaa
    43141 taagattaag gcattgacgc tgtttgccac ccactatttc gagctgaccc agctaccgga
    43201 gaaaatggaa ggcgtcgcca acgtgcatct cgatgcgctg gagcacggag acaccattgc
    43261 ctttatgcat agcgtgcagg atggcgcggc gagcaaaagc tacggcctgg cggttgcagc
    43321 tctggcaggc gtgccaaaag aggtgattaa gcgcgcacgg caaaaactgc gtgagctgga
    43381 aagcatttcg ccgaacgccg ccgctacgca agtggatggt acgcaaatgt ctttgctgtc
    43441 agtgccagaa gaaacttcgc ctgcggtcga agcactggaa aatctcgacc cggattcact
    43501 gactccgcgt caggcgctgg agtggattta tcgcttgaag agtctggtgt aataacaatt
    43561 cccgatagtc ttttgctatc gggaatatta acgacaactg acgaatcaaa taaaaatacc
    43621 ctgtataata ggaaagctta ttttacaggg taaaaccatg ccatctacac gctatcaaaa
    43681 aatcaatacc catcactatc gccatatatg ggtcgttggt gatattcatg gtgaatatca
    43741 gttattacaa tcccgcttac atcaactctc ttttttcccc gaaaccgact tacttatttc
    43801 tgtcggcgat aatattgatc gtgggccgga gagtcttgac gtcctgcgcc tgctaaacca
    43861 gccctggttt atctccgtta aaggcaacca cgaagcaatg gcgctggatg cgttcgcgac
    43921 cggcgatggc aatatgtggc ttgccagcgg tggtgactgg tttttcgatt taaatgattc
    43981 agagcaaaaa gaagctacag atctgttgct gaaattccat caccttccac atattattga
    44041 aatcactaac gacaacataa aatatgtcat cgcacatgca gattatccgg ggagtgaata
    44101 tctctttggt aaagaaatag cggagagcga attactctgg cctgttgatc gtgtgcagaa
    44161 atcgcttaat ggcgagttac aacaaataaa cggcgctgat tattttatat ttggacatat
    44221 gatgtttgat aacattcaga cgttcgctaa ccagatttat attgataccg gcacgccgaa
    44281 aagcgggcgg ctgtcatttt ataaaataaa gtagtctcat gcttcttctg tgaagcatga
    44341 gtaaccaaat gttattgcag gccattattc atttttcgct accagcaaag agagatcctg
    44401 cttcgccagc gcgcgactgg cactctccgg caaaccgtcg tctgtaataa tctgatcaaa
    44461 ctcgcttaat ggtaacgcca gccatgtcgc cacctgacca tatttcgtcg catcacagac
    44521 caaaactcgc tggcggctgg cactggcaat cgcccgtttc accgtgactt tatcttccgc
    44581 tggcgtagaa atcccccgca cactccatga cgatgcagaa ataaaagcct gatcaatcat
    44641 caggctgcgc agcatggtcg cagcggcttc cccgacacag gaacggtttt cccgacacac
    44701 tgcaccgcca gtgtgaataa ttgtgcaatt actgttgtcg agcaagtagt tcgcaataac
    44761 gaaatcgttt gtgaccacag tcagtgactc catgtgaatc agatgctgtg ctatcgctaa
    44821 cgtggtcgtt cccgcatcca gatagataca acttcccggc tgaacaagac ttgccgccag
    44881 cttgccaata gccgcttttt gcgtcattgc cagcgcagtt tttacctgat gagaaggttc
    44941 atgcgccacg cgtcccggag actggacgcc tccggacacc agcacaacgg ctccctgctg
    45001 ctccagtttt tgtaaatccc gacgaatggt catatgtgac acattcattc tgtccgttag
    45061 ttcagcaata ctgacaatgc ctttttcagc taccatctca aggatgattt ggcgacgctc
    45121 tacgggtatc aacttttgct ccttcctttg tcctgctgac attctacgct atttgcctgc
    45181 gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa taacacacaa tgttaattta
    45241 tgtgaatcag ctcaccatat cgttatcttc cagcgcttat attcacaata tcaaacaaaa
    45301 tatcacttaa attaacaagg agggcagatg aaaacgggat ctgagtttca tgtcggtatc
    45361 gttggcttag ggtcaatggg aatgggagca gcactgtcat gtgtccgcgc aggtctttct
    45421 acctggggcg cagacctgaa cagcaatgcc tgcgctacgt tgaaagaggc aggtgcttgc
    45481 ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg acgctctgct ggtgctggtg
    45541 gtcaatgcgg cccaggttaa acaggtgctg tttggtgaaa caggcgttgc acaacatctg
    45601 aaacccggta cggcagtaat ggtttcttcc actatcgcta gtgctgatgc gcaagaaatt
    45661 gctaccgctc tggctggatt cgatctggaa atgctggatg cgccagtttc tggtggtgca
    45721 gtaaaagccg ctaacggtga aatgactgtc atggcctccg gtagcgatat tgcctttgaa
    45781 cgactggcac ccgtgctgga agccgttgcc ggaaaagttt atcgcatagg tgcagaaccg
    45841 ggactaggtt cgaccgtaaa aattattcac cagttgttag cgggcgtaca tattgctgcc
    45901 ggagccgaag cgatggcact tgcagcccgt gcggggatcc cgctggatgt gatgtatgac
    45961 gtcgtgacca atgccgccgg aaattcctgg atgttcgaaa accggatgcg tcatgtggtg
    46021 gatggcgatt acaccccgca ttcagccgtc gatatttttg ttaaggatct tggtctggtt
    46081 gccgatacag ccaaagccct gcacttcccg ctgccattgg cctcaacagc attgaatatg
    46141 ttcaccagcg ccagtaacgc aggttacggg aaagaagacg atagcgcagt tatcaagatt
    46201 ttctctggca tcactctacc gggagcgaaa tcatgatcaa gattggcgtt atcgccgatg
    46261 attttaccgg cgcgacggat atcgccagtt ttctggtgga aaacggtcta ccaacggtac
    46321 aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat cgacgcactg gtgatcagcc
    46381 tgaaaacgcg ctcctgtccg gtggttgaag ccacacagca atcgctggcg gctctgagct
    46441 ggttgcaaca gcaaggttgc aaacagatct atttcaaata ctgctctact ttcgacagta
    46501 cggcgaaagg taatatcggc ccggttaccg atgcattaat ggatgctctc gacacgccgt
    46561 ttacggtctt ctctccggcc ctgccggtca acggacgtac ggtttatcag gggtatttgt
    46621 tcgtaatgaa tcaactgctg gccgaatccg ggatgcgcca tcacccggta aatcccatga
    46681 ccgacagcta tcttccccgt ctggttgaag cgcaatccgc agggcgctgt ggcgtcgttt
    46741 cggcacatgt tttcgaacaa ggtgtggatg ccgttcgtca ggagctggct cgctctaatg
    46801 attgcgctgg tgctggcatt ctggttattg gctttacgtc gcggctggag cttacaacac
    46861 accagcgaca ttatgggctc agcccttccc actgcggcgg tagtgatttt ggttactggt
    46921 gctggagggg tatttggcaa agtgctggtg gaatcgggcg tcggcaaagc ccttgccaac
    46981 atgctacaaa tgattgacct gcctctgtta ccagccgcat ttattatttc actggcgctg
    47041 cgtgcatcgc aggggtcagc aaccgtagca atcctgacaa ccggcgggtt actctcagaa
    47101 gcggtgatgg gattgaatcc gattcagtgc gtattggtga cgctggcagc ctgctttggt
    47161 gggcttggcg cgtcacatat taatgactca gggttctgga ttgtgaccaa atatctgggg
    47221 ttgtcggtag cagacggtct gaaaacctgg acagtgttaa cgaccattct cggttttacc
    47281 ggattcttaa ttacctggtg cgtatgggcg gtaatttgac cttaataaaa aggtccgatg
    47341 ggcatcggac ctttttattt agcgcttacc ttccgccagc agcggcggga tgctcggcac
    47401 cattggcgca tcatcaatat ctttctgcgt catgcggaac gcttccgggt aatgttcgcg
    47461 actggtgcgg cgtagcggtt cggtatcgcg ccaggtataa aggcaatgct ggcactgata
    47521 taccgtccag acatctttca ccggcgattt caccatcact tcaatctgtt catcggcaca
    47581 acgtggacaa atcatcttgt tctccttatt tacgtgcagc cagcatagtg gtcagttttt
    47641 cagcccaggc tttggtttcc ggcaaatcca ccaccggctg gctgtagtga ccacggttgt
    47701 ccggggcgac aggcgtggta gcgtcgataa tcagcttgtc ggtgatcccc gccgggcttg
    47761 agccagggtc gagttccagc acggacatat tcggcaactg caccaaatcc cctgccgggt
    47821 ttactttcga ggagagcgcc cacatcacct gcggcaggtt gaacgggtca acgtcttcat
    47881 cgaccataat caccatcttt acgtagccca gaccgtgcgg cgtggtcatc gcacgcaggc
    47941 ccaccgcgcg ggcaaagccg ccgtagcgtt ttttggtgga gataatcgcc agcaggccgt
    48001 gggtgtacat ggcatttacc gcctgcactt ccgggaactc ggctttcagt tgttgataca
    48061 gcggtacgca ggtggctggc cccatcaggt agtcgatttc ggtccacggc ataccgagat
    48121 agagtgattc gaaaatcggt ttgctgcgat aagagacttt atcgatgcgc accacggtca
    48181 tgttgcgccc accggagtag tgcccggtaa actcaccgaa cggcccttcg atttcgcgtt
    48241 tacggctttc gataacccct tcgaggatca cttctgaacc ccacggcaca tcaaaaccgg
    48301 tcagcggggc ggtggcgatc gggtacgggc tttcacgcag cgcgcctgcc atttcatact
    48361 cagactgatc gtatttcagc ggcgtggccc ccatcagcgt gatgatcgga tcgttaccga
    48421 gcgtgatcgc aatcggcaga tcttcaccgc gctcttctgc tttatgcaga tgcagggcga
    48481 tatcgtgcat cggcaccggt tgcaggccga gcttacgctt gcccttcact tccatgcggt
    48541 agatgccgac gttctgcttg ccgaagttat ctgggtcgag cggatcgcgg gaaaccacgc
    48601 aggctttgtc gagatagaaa ccgccatcgc catcgtttaa acgaaacagc ggcaggatat
    48661 cgaacaggtt aatctcttcg ccatcgacgg tgttctgcgc ccaggctgga ttggcgcggc
    48721 gctccggggc aatcgggaag ttatcccagc ggcggataaa ctcatcaatc tgctttttga
    48781 ccggggtgtt tggtggcagg ccaagggaaa tcgcgtggtt ctgccaggaa ccgatggtat
    48841 tcatcgccac gcgggcatcg gtaaagccgc gaatattatc aaaccacagc gcgggcgcgc
    48901 cgtcgccgat acgcccggtg gcgttagctg ctgctgccag atccggctca gcgttcacct
    48961 cttcgctgat tttcagtaac tggccgtggt catcaagcgc ctgtaaaaag ctgcgtaaat
    49021 catcaaatgc cattattcat tctcctgaga aaaattccgg gcctgcggca atccttgcca
    49081 gcgcctggca tggggatgtt cgaggccaaa ttgatccagc acgcgggcta ccacatggtg
    49141 gacaatgtca tctaccgttt cgggatggtt ataaaaggca ggcatcggcg gcaccatcgc
    49201 cacgcccatg cgtgaaagtg cgagcatatt ttcgagatgg atggtgctaa gcggcatttc
    49261 acgcggcacc agcaccagtt tgcggccttc tttgagcacg acgtccgccg cgcgccctac
    49321 caagccatcg gcgtaaccag cgcggatacc ggcgagcgtt ttcatactgc acggaataac
    49381 gatcatgccg tcggtacgaa aagaaccgga tgagatgatc gccgcctgat ccgccgggtt
    49441 atggctgaag tctgcgaggg cagcaacatc gcgagcgctg taaggcgttt ccagttcaat
    49501 ggtggttttc gcccacttcg acatcaccag atgagtctcg acattcggca tatcccgcag
    49561 cgcttgcagt aatgccacac caagaggcgc accggtagcc cctgtcatcc cgacgatcag
    49621 tttcattgct tatcctctta attgttcgta tacgaacgtt attattaaca tactccttca
    49681 tttccacagc ggcaaggatt gaaaagtaac aacatcttct tcgtcactaa aaaaccgcta
    49741 tgatagcgac agagaattag cccggagttt acatggcgtt acgaaataaa gcgttccatc
    49801 agttacgaca gctttttcag cagcacacgg ctcgctggca gcacgagtta cctgacctga
    49861 ccaaaccaca gtacgcggtg atgcgcgcca ttgctgataa acctggtatc gaacaggtgg
    49921 cactgataga agcagctgtc agcaccaaag caacgttagc agaaatgttg gcaagaatgg
    49981 aaaatcgtgg cttagtcaga cgtgaacatg accccgctga taagcggcga cgctttgtct
    50041 ggttgactgc cgaaggcgaa aagatacttg ctgcggcgat acccattggt gacagcgtgg
    50101 atgaggaatt tttggggcgt ttgagtggag aagagcagga gctgtttatg cagctggtgc
    50161 gcaagatgat gaacgcatag ggggcaaatt tccggatgcg acgctatcgc gtcttatcca
    50221 gactacaaat acaccgactc accaaccgaa agaaaaaagg ccagcctcgc ttgagactgg
    50281 cctttctgac agatgcttac ttactcgcgg aacagcgctt cgatattcag cccctgcgtt
    50341 tgcaggattt cgcgcaaacg gcgcaggcct tcaacctgaa tctggcgaac acgttcacgg
    50401 gtgaggccaa tttcacgacc tacatcttcc agtgttgccg cttcgtaccc cagcaaaccg
    50461 aatcgacgtg ccagcacttc acgctgtttg gcgttcagct cgaacagcca tttgacgatg
    50521 ctctgcttca tatcgtcatc ttgcgtggta tcttccggac cgttctcttt ttcatcggcc
    50581 aggatgtcca gcaacgcttt ttcggaatca ccacccagcg gggtgtctac cgaggtaatg
    50641 cgctcgttaa gacgaagcat acggctgacg tcatcaactg gcttatccag ttgctctgcg
    50701 atctcttccg cacttggttc atggtccagc ttatgggaca actcacgtgc ggttcgcagg
    50761 taaacgttca gctcctttac gatgtgaatc ggcaaacgaa tagtacgggt ttggttcata
    50821 atcgcccgtt caatcgtctg gcgaatccac caggttgcgt atgttgagaa gcggaaacca
    50881 cgttccgggt caaacttctc taccgcgcgg atcagcccca ggttgccctc ttcgataagg
    50941 tccagcaacg ccagaccacg attgccataa cggcgggcaa tttttaccac cagacgcaag
    51001 ttactctcga tcatccggcg gcgagaggcg acatctccac gcagtgcgcg acgcgcaaaa
    51061 taaacttctt cttcggccgt taacagtggt gaataaccaa tctcaccaag gtaaagctga
    51121 gtcgcgtcca acacacgctg tgtggctccc tgcgataaca gttcctcttc ggccaaatcg
    51181 ttatcactgg gttcctcttc tactaaggcc ttttcgtcaa aaacctcaac tccgttctca
    51241 tcaaattccg catcttcatt taaatcatga actttcagcg tattctgact cataaggtgg
    51301 ctcctacccg tgatcccttg acggaacatt caagcaaaag cctggttccg ccgatttatc
    51361 gctgcggcaa ataacgcagc gggtttacgg atttcccctt gtaacgaatt tcaaaatgca
    51421 agcgtgttga actggttccg gtgctaccca tggttgctat tttttgcccc gccttcactt
    51481 cttgttgttc ccggaccagc attgtgtcgt tatgggcgta ggcactcagg taatcatcat
    51541 tatgtttgat gataatcaga ttaccgtagc cgcgcagcgc gttaccggca taaacaacgc
    51601 ggccatctgc ggtcgcgata attgcctgtc ctttgctgcc tgcgatatca atccccttgt
    51661 tgcccccctc agaagcgcca aaggtttcga tcactttgcc ctcagtcggc cagcgccagg
    51721 tggagatagg cgtactggtt gatgtactgc tgacagttgg ctcggttgtg cttgctgttg
    51781 gtaccgttac aggcgctgtg accgtggtcg cagctggctt gttgttcggc aacattttgt
    51841 tagcactctg ttcacccgaa gattcagaat acgtaattgt cggttgcgac gcaacagcaa
    51901 cggtggaatt ttgtgcaggc ttgatcacaa ctccttgctc tgctgcgtcg gcctgggtaa
    51961 tggcatttcc gccagtgatt ggcgtaccgg aagcattacc cacctgcaag gtctgaccaa
    52021 cgttcagcgc gtatggtgcc tgaatattgt tgcgctgagc aaggtcacgg aaatcgttgc
    52081 cagtaatcca ggcgatatag aaaagtgtgt cgcctttttt cacggtatag gtactgccgc
    52141 tataactgcc tttcggaatg ttcccatact gacggttata gacgatgcgt ccgttttcca
    52201 tctgtaccgg ctgctgagct actggctgga tttgcggttg ttgagtagcc tgaatttgtg
    52261 gctgctgtac cggctgaatt tgcggttgct gggctgtaga cgtcgtcccc attttcggcg
    52321 gcggcgtaat caacatacca gaattggtat ttgcaggcgc attgccatta acggagctga
    52381 ccggtgccgg tggatttgaa gtgtcagaac agcctgccag ccatagcgaa accagtgaca
    52441 aagccgcaat gcggcgaacg gtgaattttg ggcttcccgc gctcatttat cccccaggaa
    52501 aaattggtta ataaccagtg acataattac cgtgcaaggc accctactga acactggaaa
    52561 agatgttcac gatacgctga cctgcggcaa aataaccagg aaaaatccag gtatttcctc
    52621 acgttttaag ccagctcacc cttcactaaa gggacaaagc gcacggcctc cacggtatcg
    52681 ataataaatt cgcctccccg acgacgcacc cgtttcaaat actggtgctc ctcccctacg
    52741 ggtaagacga gaatcccgcc ttcgtccagc tgcgtcatta gcgcagttgg aatttccggc
    52801 ggtgccgccg taacaatgat agcgtcaaac ggcgcgcgtg cctgccaacc ttgccatcca
    52861 tcgccatgac gggttgaaac attatgtaaa tcaagatttt tcaggcggcg acgcgcctgc
    52921 cactgcaaac ctttaatccg ttcgaccgag caaacatgct ggacaagatg cgccaggatt
    52981 gccgtttgat atcccgaacc ggtgccaatt tccagcaccc gcgactgcgg cgtcagctcg
    53041 agtaattcgg tcattcgcgc caccatatac ggctgcgaaa ttgtctgccc ctgacctatc
    53101 ggcaaggcga tattgtccca ggctttttgt tcaaacgctt catcaacgaa tttttcacgc
    53161 ggcacggcag caagtgcatt cagcacctgc tcatcctgaa taccttgcgc acgtaattga
    53221 tccagaagtg cttgtacgcg tctgcttacc attgcgtgcc aactcccacg ctgtttaacc
    53281 agtctgaaac cacatcttgc gcgctatgcg cagttaaatc cacatgcagc ggcgtgatgg
    53341 agacatagcc ctcatctacc gcagcaaaat cggtccccgg accggcatca catttaccgc
    53401 ccggcgggcc aatccagtac agcgtattgc cgcgcggatc ttgctgcggg atcacctgat
    53461 ctgccggatg tcgtgtaccg cagcgcgtca cgcgaatacc tttgatttga tccaagggta
    53521 aatccggaac gttaatatta agaatacgcc cggtgcgcaa cggctcttta cacagtgcgc
    53581 gcaaaattga acaggttacc gccgcggcag tgtcgtaatg tttatgcccg tcaagcgaga
    53641 cggcaagcgc cggaaaacct aaatgacggc cttccatcgc ggcggctacc gtaccggaat
    53701 aaataacatc atcccccaga ttcggcccgg cgttaattcc ggacacaaca atgtccgggc
    53761 gcggacgcat cagagcattc acgccaagat agacgcaatc ggtcggggtt cccatttgca
    53821 cagcaatatc accattttca aaggtaaacg tgcgcaggga ggattccagt gtcagagaat
    53881 ttgaagcgcc gctgcggtta cgatcggggg cgaccacctg aacgtcagca aactcacgca
    53941 aggctttcgc cagcgtttgt ataccgggtg catgtacccc gtcatcatta ctcagcaata
    54001 tgcgcataat cacctgttgt gttgataagt tccctgacaa cgctggttgc aaaactaccc
    54061 gccggaagcc agaaacggat ctctacggtg acgtcatccc accaattcca gcttaattgt
    54121 tgcggataca gcagcatcgc tctgcgcgcg gcttcaactt tttcgcgcac cagtaaagct
    54181 tgtaattcag tttctgcggc gacagctgct tgttcgaatg ccagcgcttc acgctgagtt
    54241 ccccattcgc cactgcccgg caatgcggcg gttatcatca gctctttatc gttaacgcga
    54301 cgctgtaatt ccgccagttc ttcggtggtt gcgacaaacc agctaccacg tccggctaat
    54361 tgtagcgcat cgccgtcaac aacttgatta acgtctgctt ttttgaggcg ctcagcaaca
    54421 atctgattaa acaacgcact gcgggctgcc gacaaccaaa aactccgttt attgcgatcg
    54481 cgcaccggag tattggtttg cgcccagcgc agcgcgccct gcaagttgct accgccaatc
    54541 ccaaaacgtt gggcaccgaa gtagttcggt acacctttta cgcaaatatc gatcagacgt
    54601 tgttcaacgt catcgcgatt gctcacttcg cgcagaacca gggtaaaggc gttacctttc
    54661 agcgccccta aacgcagctt gcgcttgtgc cgcgcatact ccagcacctg gcagccttcc
    54721 agttgaaagg cgctcagatc gggcatctcc ttgcccggca cgcgagcaca taaccactgt
    54781 tccgtaacag catgtttatc tttttgccca gcgaagctga cttcacgggc atgaattttc
    54841 aggaatttcg ccagtgcatc cgccacaaaa cgggtattgc agccgttttt gaggattcta
    54901 accagaatat gctcaccttc accatcaggc tcaaagccca aatcttccac caccacaaag
    54961 tcttccggat tggctttcag cagcccggtg ccttgcggtt taccgtggag gtaagtgaga
    55021 ttatcaaact caatcatttt gttgccttaa tgagtagcgc caccgcttca caggcaatcc
    55081 cttccccacg tccggtaaat cccagttttt ccgtagtagt ggctttcacg ttaacatcat
    55141 ccatatggca gccgagatct tcggcaataa acacgcgcat ttgtggaatg tgcggcaaca
    55201 tcttcggtgc ctgagcgatg atagtgacat cgacgttgcc aagggtataa cccttcgcct
    55261 gaatacgacg ccaggcttcg cgtagcagct cgcggctatc ggcaccttta aatgccggat
    55321 cggtatccgg gaacagtttg ccgatatccc ccagcgccgc cgcgccaagc aatgcatcgg
    55381 tcaacgcatg gagcgccacg tcgccatcag aatgcgccag caatcctttt tcgtaaggaa
    55441 tgcgtacgcc accaatgata attgggcctt caccgccaaa ggcgtgtacg tcaaaaccgt
    55501 gtccaattcg cattatgtat tctcctgatg gatggttcgg gtgaggtaaa actcggccag
    55561 tgccaaatct tccgggcgcg tgactttaat gttatccgcg cggccttcga ccaactgcgg
    55621 atgaaatccg caatattcca gcgccgaggc ttcgtcagta atagtcgcgc cttcatttag
    55681 agcgcgcgtc agacagtcat gtaacagctc acgagggaaa aattgcggcg tcagcgcgtg
    55741 ccataagccg ttgcgatcaa cggtatgagc aatggcattt ttgcccggtt cggcacgttt
    55801 catcgtatcg cgcactggtg cggctaggat ccctcccgtg cggctggttt cgctcaacgc
    55861 caacaatcgc gcgaggtcat cctgatgcag acaaggacga gcggcgtcat gcaccaatac
    55921 ccactgcgcg tcgccagcgg ctttcaaacc tgccagcacg gaatcggcac gctcatcacc
    55981 gccatctaca acggtgattt gcggatgatt cgccagagga agttgtgcaa aacggctatc
    56041 gccaggactt atggcaatga cgacacgttt cacccgggga tgcgccagca gcgcatgcac
    56101 cgagtgttca agaatggttt gattaccgat tgagagatat tgcttaggac attccgtttg
    56161 cattcgacgg ccaaatccgg ccgccggaac cacggcgcaa acatccaaat gagtggttgc
    56221 catgttaatt cccgggctga tttatcgatt gttttgcccc gcagactgtg cgcgcttcga
    56281 cgcgtcaggc accagacgat aaaaagtttc gcccggcctg gtcatgctga gttcattacg
    56341 cgcacgctct tcgagcgcct cctggccgcc attgagatcg tcaatttcgg caaaaagttg
    56401 atcgtttcgc gctttaagtt tcgcgtttgt agcttgctgt gccgccacat catcattgac
    56461 gcgggtatag tcatgtatac cgttcttacc gaaccacagc gaatactgta gccagaccag
    56521 aatagccagc aacagcagcg ttagtttacc catcctgccc cctgaaaaac ggcatcatca
    56581 tcccatgcat ccgaagacga ctccacatcc tctgttgggg ataccgcgac aacgcgggca
    56641 aatgtaccac atttgtccat tgttacgtat acccagggcg tgcagaacat aatctcatta
    56701 ttagttacgg tttgaattat gaacagagga gacaagaaag tacaaattag cccagtagcc
    56761 acataaacag tgcgccaaac ataatgccta ctgtcaccag ggtgaaaaca atactgtagc
    56821 gtagctttcc gtccatcaat gaatgcagcg caatccccac cactaccgcg acaggcatca
    56881 gtgccagaaa gaaaggccag gtgtagataa agaagaacag cgtgttagag ccataaatca
    56941 acatcggcat cgccagcgca aataaccagg agataaaacc gaccacggca ccaggcagtg
    57001 accatgtggt ttcttcatcc tcagtaaggc tgtcgttatt tgttagtgta atgttatggc
    57061 tattacgcat atttgatcct gttactttga cgaaccgggc atggaaaccc ggtggtgtct
    57121 caggatctga taatatcgtt ctgtctcaac agatctaata attgctgtac caaatttgtt
    57181 actaattgtt caccattgag atgaatttct gccgattcag gcgcttcgta aacggaatct
    57241 attcccgtaa agttgcgcag ttcaccggca cgcgctttct tatataagcc tttgggatcg
    57301 cgggcttcgc aaatcgccag cggcgtatcg acaaacactt cgataaagcg cccttctcct
    57361 acgcgttcgc gaaccatctg gcgttcggcg cggtgtggcg agataaatgc ggtcagcacc
    57421 accagtccgg cttcaaccat caaattcgcc acttcaccga cgcgacggat attctcttta
    57481 cgatcggcat cgctaaaacc gagatcgctg cataatccgt ggcgaacatt gtcgccatcc
    57541 agcagatacg tactgacgcc gagtttatgt aacgcctcct ccagcgcccc ggcgaccgtt
    57601 gatttaccgg acccagagag gccggtaaac cacagcacta caccacgatg accgtggtgt
    57661 agctcgcgtt gttgcacagt gaccggatgg ctatgccaga cgacgttttc gtcatgcagc
    57721 gccattattt atcccccagc aaatcgcgcg cgccccagtg cggaaagtgg cgacgaacca
    57781 gggcattcaa ttccagttcg aatgcactga attcagatga cgcaacggcg gcctcgtttt
    57841 tcggctcatg caccataccg gcaccaacag tcacgttgct caggcgatcg ataaaaatca
    57901 gcccccccgt caccggattt tgttgataac gatctaacac cagcggctcg tcaaaagtga
    57961 gatccacgag gccgatccca ttcagtggca ggttttcaac ttcacgctgg gtaaggttat
    58021 taatatcaac ctgatagcga atgccatcaa tacgcgcgcg cgtcttctta ccggcaattt
    58081 tgatgtcgta actctggccc ggggaaagcg gctgttccgc catccatacc acatccaccg
    58141 acgcgctctg caccgccggt aacgcttcgt ctgccgccag cagcagatcg ccacggctga
    58201 tgtcgatctc atccgtcagc accagggtga tcgcttctcc ggcaaaggct tcttcgcgat
    58261 caccatcaaa agtcacgatc cgcgcgacgt ttgattccac accagagggc agcactttga
    58321 cacgttgccc gacttccacg cgaccggatg ccagcgttcc ggcgtagcca cgaaaatcga
    58381 gattcgggcg attaacgtac tgcaccggga agcgcattgg ctgagcatcc accactcgct
    58441 ggatctccac ggtttccagc acttcgagca gtgtcggacc gctgtaccac ggcatacttt
    58501 cactttgcga agccacgttg tcgccttcca gcgcagagag cggcacaaag cgaatatcca
    58561 gattgccagg taactgttcg gcgaaagtca ggtagtcttc acgaatgcgg gtgaatgtct
    58621 cttcactgta atccaccaga tccattttgt tgatcgctac gaccagatgt ttgatcccca
    58681 gcagcgtgga gataaaactg tgacgacggg tttgatcgag cacgccttta cgggcatcga
    58741 tcagtaagat cgccagctca cacgttgagg cgccggtcgc catattgcgg gtatactgct
    58801 cgtgcccggg ggtgtcggcg ataataaatt tacgcttctc ggtagagaaa tagcggtagg
    58861 ccacatcaat agtgatgccc tgttcgcgct cagcttgcag gccgtccacc agcagagcca
    58921 gatccagttt ttcgccctgg gtgccgtgac gcttactgtc gttatgcagc gatgagagct
    58981 gatcttcgta gatttggcgg gtatcgtgca gcaggcgacc aatcagggta cttttgccgt
    59041 catcgacgct accacaggtc agaaaacgca gcaggctttt atgttgttgc gcaatcatcc
    59101 aggcttcgac gccgccttca ttagcgattt gttgtgcaag tgcggtgttc atcttaaaaa
    59161 tacccctgac gttttttcag ctccatagac cccgcctggt cgcggtcaat cacgcggccc
    59221 tgacgttcac tggtggtgga aaccagcatc tcttcgatga tctccggcag tgtttgtgca
    59281 tttgactcca ccgcaccggt cagcggccag cagcccagcg tacggaaacg caccatccgt
    59341 tttttaatca cttcgcccgg ttgcaggtcg atacggttgt catcaatcat catcaacata
    59401 ccgtcgcgtt ccagaaccgg acgttccgca gcgagataca gcggcacaat gtcgatattt
    59461 tccagccaga tgtattgcca gatatcctgc tcggtccagt tagagagcgg gaaaacgcgg
    59521 atgctttcgc ctttgttaat ctgcccgtta tagttgtgcc acagctccgg gcgctgattt
    59581 ttcgggtccc agcgatggaa gcgatcacgg aaagagtaga tacgctcttt agcacgggat
    59641 ttctcttcgt cgcggcgcgc accaccgaag gcggcatcaa aaccgtattt attcagcgcc
    59701 tgcttcaggc cttcggtctt cataatatcg gtatgtttcg cgctgccgtg cacgaatgga
    59761 ttaatcccca tcgccacgcc ttccgggttt ttatgcacca gcagctcgca gccgtaggct
    59821 ttcgccgtac gatcgcggaa ctcatacatc tcgcggaatt tccagccggt atcgacatgc
    59881 agcaacggga aaggcagcgt acctggataa aacgccttgc gcgccaaatg cagcatgacg
    59941 ctggaatctt taccgataga gtagagcatc accggatttg agaattctgc cgccacctcg
    60001 cgaataatgt ggatgctttc cgcctccagt tgccgcaggt gagtaagtcg tttttgatcc
    60061 ataaccgttc ctttgcaata ccgctatttt cttgccatca gatgtttcga ctatagggag
    60121 cgtaagagaa cgaatgaaat taccaattag aatgagtagt tccttaacgg aataacgatt
    60181 tggcaaagct aatatcaaaa agtgcttaag gcaccggatt tcgggcgttt aggaagattt
    60241 gaaattgttt tagcgcagcg gcagtttcat actatggcgg taaaaaaatt tgcatggtat
    60301 ttaaggactc actatgtttt ccgcattgcg ccaccgtacc gctgccctgg cgctcggcgt
    60361 atgctttatt ctccccgtac acgcctcgtc acctaaacct ggcgattttg ctaataccca
    60421 ggcacgacat attgctactt tctttccggg acgcatgacc ggaactccag cagaaatgtt
    60481 atctgccgat tatattcgcc aacagtttca gcaaatgggt tatcgcagtg atattcggac
    60541 atttaatagt cggtatattt ataccgcccg cgataatcgc aagaactggc ataacgtgac
    60601 aggaagtacg gtgattgccg ctcatgaagg caaagcgccg cagcagatca tcattatggc
    60661 gcatctggat acttacgccc cgctgagcga tgctgacgcc gatgccaatc tcggcgggct
    60721 gacgttacaa ggaatggatg ataacgccgc aggtttaggt gtcatgctgg aattggcaga
    60781 acgcctgaaa aatacgccta ccgagtatgg tattcgattt gtggcgacca gcggcgaaga
    60841 ggaagggaaa ttaggcgctg agaatttact caagcggatg agtgacaccg aaaagaaaaa
    60901 tacgctggtg gtgattaatc tcgataactt aattgttggc gataaattgt atttcaacag
    60961 cggtgtaaaa actcctgaag cagtaaggaa attaacgcgc gacagggcgc tggcaattgc
    61021 gcgcagtcac ggaatagccg caacgaccaa tccgggtttg aataaaaatt atccgaaagg
    61081 cactgggtgt tgtaatgacg cagaaatatt cgacaaagcg ggcattgctg tactttcggt
    61141 ggaagcgact aactggaatc ttgggaataa ggatggttat cagcaacgcg caaaaacagc
    61201 cgcattccct gcgggaaata gctggcatga cgtaagactg gataatcaac agcatattga
    61261 taaggcactt cctggaagaa tagaacgtcg ctgccgtgac gttatgcgga taatgctacc
    61321 gttggtgaag gagctggcga aggcgtcttg atgggtttga aaatgggagc tgggagttct
    61381 accgcagggg cggggaattc taagtgatat ccatcatcgc atccagtgcg cccggtttat
    61441 ccccgctggc gcggggaaca ccagattatc ttctttttca atccagccac cgacggttta
    61501 tccccgctgg cgcggggaac acgattgttt tcgcgcccgt gtcattgtcg aggtcggttt
    61561 atccccgctg gcgcggggaa cacgtgaccg tcggtgttac gctgaactgc tggaacggtt
    61621 tatccccgct ggcgcgggga acacacgcgc gtaccggatc gcggacaaca aattgccggt
    61681 ttatccccgc tggcgcgggg aacacgcaaa ttaccgccgg gctaacgttt gatctcacgg
    61741 tttatccccg ctggcgcggg gaacacatgt cgagcgcgtt gacagctata ttcgccgtcg
    61801 gtttatcccc gctggcgcgg ggaacacatg tcgagcgcgt tgacagctat attcgccgtc
    61861 ggtttatccc cgctggcgcg gggaacacgg tcgctggtca aattcccaat ctgagcagtc
    61921 cggtttatcc ccgctggcgc ggggaacacg cagatttcaa acaggcgcgt aatcccgcgt
    61981 ttcggtttat ccccgctggc gcggggaaca cactaagcat acataactgt ttttaaacaa
    62041 atttattccg catcaacaat ctaccaacta aattaaaaca tttccttatt tttaaagaac
    62101 ggataactta ttgattatca acaggaagaa aagaaaccaa acgtaaccca tccaaatcca
    62161 ccggaatacg tctgttttct ccccaggtct gaaattcaaa acccgactcg gtattggtcg
    62221 cccaggccat caccacattt ccgcaaccag ccagttgggt aatttgctgc cagatcatct
    62281 cccgaatacg ttttgatgta tcaccaacat acacaccggc acgcacttcc agtagtcaga
    62341 ttgcgagccg tccacgtaag cgcggcggga cattttctgt aacaaccacg accatgctca
    62401 tcagccgcgt cctcgatgac cgctatcgcc caacgtttca ggttcgggga tggctggcgg
    62461 taacatatcc ggcgcaggtt gtggcggttc aatttcacct gcggcaagga cttcctcaat
    62521 taacggtatt aatttgcccg ttaacttagt gctacggaaa atatcgcgac aggctaatct
    62581 gacttcttta tcaggttctg cgggttgcct cgctgctatt tcaaatgcct ttggcacaac
    62641 cgaatcaaat ttaatgatat cggctatgtc ataaacaaat gaaagcggtt tgccactatg
    62701 gataaaccca atagcgggcg catatcccgc ggctaatact gccgcttcag atataccgta
    62761 cagacatgat gtagcagcac tgatgcagcg attcacaaca tcgccttttt cccagtcttt
    62821 aggatcgtat ttgcgaccat tccatttcac accatattgt ttcgccagta atgcatacgt
    62881 ctggcgaacg cgagatccct caattccacg taactgctcc actgaacggc gagctggcgg
    62941 tggttcacga aaacgtaatt catacatttt gcgcaccacc ttcaaccgta gatcttccgt
    63001 taaagccagc tttgcctggt agagtaattt atctgcccgc gccccaccgg gttgtccgga
    63061 ggagtaaacg cgaacgcccg cttcaccgac ccagaccagc agtgttccca ccgtggcggc
    63121 cagatgcacc gccgcgtggg aaactctcgt tcctggttcg agcataatgc aggcgaccga
    63181 tcccaccgga atatgcgttc ggatccctgt tttgtcgatc agcacgaaag cgccgtccag
    63241 tacgtcgatc tgaccgtact ggaggaagat catggaggtg cgatctttta atgggatcgg
    63301 gctcagtggt acaaacgtca cgcctcttct ccgggtttga tcagcatcag accacagcga
    63361 acgcccggct tttaccatac ccctggctca ggcgttgcac aaataaccca ggatcggtga
    63421 ccgtaagcat ccccgtataa tccacgctac tgaactggat cagttgccgg gagttttccc
    63481 gccgcagttg ctgttgtcgg taggcatcaa ccgaggtatc cagcagtgta aaaccgcttc
    63541 tctccccctg cgctgccagc cagtccagcg ccgcctgttg ttgatgcaac cagacatcac
    63601 ttccttccgc ctgccccctc acctgccgtt tcgcctccat cagcagatcg tggcgcttgc
    63661 ccgctttaca gattgttgga ttcgcccgca ggttgaaaca aagttgttgt ccggtacaca
    63721 gctcgggcgc aaatgaccgg cattctatgg tgaacgtatc actttccgcc gggcgttcct
    63781 gcgacagtac aaaaaagcga aacgcgccct ggagttcttc ccgacgataa agaaattgcc
    63841 tttctttgcc gccagggaag agatcccaca gccactgatg catcacatat tcaccgcgat
    63901 ccaccagatg cagcaactgc gcaggcgaaa gctggccggt atgtaaggtt attcttgaga
    63961 ggtacatggt tcctccttgc tgagccacgg cccctgattg acggtgcgtt ccccaaacag
    64021 ccactgctga cgatttaaag gaacatcccg gcggcgtaat attttgctcg cgaccaggcc
    64081 gtcgtgtttc ccttcccacc agcattcatc ctgaagtttc gggagtgaga ctttcagttt
    64141 atggaaatga tcctgatact gttggtatgc attacataag gcatcacacg cgttgccttc
    64201 gagcagtaac ggcgcaagag gtaatgccag cggatgactt tttcgcccca gataaagcgg
    64261 aaaaactgga tggcgtaaac cgtcctgcaa ctgttcgagg ctgtaaggcg catcggctgt
    64321 tgttgccacc gccaccatcc accaggcatc ggtgtagtag tcgcgccggg agataatcgc
    64381 gctcaaaagc tcagggtcgc tcaactcttc gcgacggctg aaataacgtg ctttacgcac
    64441 ctcttttggc atctggaccg tgtgataatc ccgtgcccag cacgggttac ggctggcgca
    64501 aaccaccagt gaatagtggc gattaaacgc gtttaagcgt tcggtatcat cacgccgaat
    64561 ccctaccccg gcagccagta gccccagcaa tgctgagcgc gaaggaagtt catgggtatg
    64621 acgaacttca cctggagcat cgacccccca ggacgccatc ggcccatgaa gctgaaaaat
    64681 cagatattgg ctcataagcc gccccttacg cgcagataaa gtccagcacg tccttcatgc
    64741 ttccctgttt gttcatcacg tcaaaacttg cgcattcggt cttctgttcg tagaccgtat
    64801 tcatgttttc gcgaagcgtt gtaatacgct gcaccgccac atctaactga cgtgtgccat
    64861 taatgggttc atagaaagcc gccgccagag agcgtggttg atcggtgcct ttttctgcca
    64921 gcgcccagga ggcgtaggca cggctggcaa agctgttctg tttgccggtt ggggagactt
    64981 taagtgcggc ttccgtaaag gcacgtatgg tctgattagc taacgcttcg tcaccgccaa
    65041 ggttttcgac cagcagatct ttatcaatgc agatataggt gtagaacagt gcagaaccga
    65101 atccggtttc accaagatgc ccggcaccgg catcttcaga agcctggcgc aaatcatcaa
    65161 cggcggtgaa aaaatcatct tcgacaatcg tttcactgac accaaatgcg tgcgctacct
    65221 ggcaggcggc ttcaacatta aactcgggtt tattcgccag catacgacca aacatggcaa
    65281 tatctaccgc catacggtct ttgcgtaaca acgcgagatc ttcctctttt ggcgcgcgct
    65341 tttcttctgc aagctgatgg gccagcgctt ttacggcgtc aaattctgcc gggctgatat
    65401 ggactaactg ttcagtttcg gcgttagtga gcggatcttt tggttttttg tcgtttttag
    65461 ctttcccaag ataatctgca atttttgccg cccattcgat ggcttttttc tcttcgattc
    65521 ctttctcaat caggatagtt gccgcctcac gcgcaatacg cccactgcga ataccaatat
    65581 ggcccgccag tgcctgttca aaaagtgcag aagtgcgcca cgcacgtttc agactttgcg
    65641 aggaaacgcg cagacgcgtt gctccaccca gcaccacagt ttttggcgca ccggtatcat
    65701 cacggttaag gttggcagca gggtaagcgg ttaacaaatg aagctgaata aatgttgtca
    65761 tgagatagtc ctttattgat tttgttcgtt atcggcgtcg ccagcctggt aatattccag
    65821 cgcccagcgg atacggataa attcggttgg ccgctgctgg cggcggtgat gattgagcag
    65881 gtcgtcgctc tcctggcacc aacggaagac cccctctgcc agagagtcaa gattgacgga
    65941 accgtttaat aatctgactg cgcggcgtaa ctggcgcagt aattcatccg gtgtttttac
    66001 tgccgacaga cgagtaaaac gcccctttga cattaccgcc gccagctgcg cggcaaaagg
    66061 ctgccgttca tcaatagctt taacattggc gctaactgcg gctatcagcg ccagtgcggt
    66121 gatacgccac tcaggttcat cctgccactt tatttgtctg ttcttcagaa acaggcgaaa
    66181 tccatccgtc agacaaacat cattaaccgt cgtactacgc cgcagactgg cacgttcgcc
    66241 acgtttctcc tgcaattcct cgtgccattt gcgcagcgtg gctttgtgct cctcttttac
    66301 aatactcatt cagcagcctc ctgctttttc tccctggcgg ctttagcact ttgcttctcc
    66361 gccgatgttg taaaatattt cttgcgcgcg gtcatgacgc gttccagatc aacgggctca
    66421 taaggattgg tgaatacacg ctcgtcaaaa tcctgacgtg cgaataacca aatttcctta
    66481 ttccatttgc tgagtaattc atccgcatcc tgaccttctt caatttggcg cactaacctc
    66541 agaaagcgat gctgagtttt gttccagaag tcgatatcca caaaactaaa atcccccctt
    66601 gcaccttttg gatcggagaa ccatgcttct ttcaatgcac tccgtaacag acttagaatc
    66661 cgtgaagctg tttgcgcagc cagccgcagc ttcggtatct gaccttcttt tttatcgagc
    66721 agcagcggga aatggtgttc gtaccaacag cgcgctttca tgttgtcgaa atcatagcca
    66781 aatccccaca ggcccacttt tgcctgcttg agactgctgg cattgaagag cttcaccacc
    66841 agcgcgggaa gttccgtatt gttttctgat ttacccgttt cgataaggcc taaccagtca
    66901 cgccagatta aaccgcccgg ttgtggttta acggagtaaa actcgccacc ctctttaagt
    66961 ggtacacggt aaggcgttaa gggatgctgc cacatggcat aattcgcacc gtaatttttg
    67021 gtcgtcatca ggctcaggag cgcgtcgctc tgctcaccgc aaatatcgca gttgccgact
    67081 gtcgtggtat taaaatcaat acgaatacgg cgaggcattc cccagtacgc ctggagttta
    67141 ttgacctgct catcggttac caccgcaccg gccagttcgc tggtgcgcgt cgggccaagc
    67201 caggggaaaa ccagatcgtc aaattttttg ggtagcggta aatcggcttc atcctgcggc
    67261 atgacattga tccacagttt gcgccacaag ggagtttgtt gattaccctg atattcctgt
    67321 aattcaatca gagtggtcat cggcccaccg ccgcgtaaac cggtgcggta gcctttgcca
    67381 cctgacggcg cattcaactg tagggagaac agagctaacg cagaacaatg tgggcacagg
    67441 tattccgtta caccacgctt gataaagtgg tctttattaa acttcgttgt ttgagcgccg
    67501 ggaatctcag gcagtagcga agcgaccgga actttatctc cggtgagcgc ctcgaaatcc
    67561 tgcataaatg aaggggaatc tggcccaaac tggaaagcat gttctaatga ctgcaatgct
    67621 tcccgtagct tttcagcttc cagtccgtct tcccagatat catcccaacg acgatgatct
    67681 tttggcgcga agctggtttg tagtagcccc agcaaaaact gccataccgc accctggaga
    67741 tctgcccgcg gcgcagaaat atcgacaaca ttttcatccg ccagatcgac tggcgccagt
    67801 ttgcctgtcg ttccgtcttt aaaacgaacg ggcaaccacg gggttgtcag aagtgaaaac
    67861 gagttcatac gcatcctctc gtaaaaaaga ccatcccagg tcggttgtcc atctttccat
    67921 ccttttcggt gtatcaacta ttcagagagt cctgtcgcag acatctcaac caaccttgcc
    67981 aatcaatcct tccgtagccg aatacccgca actttcgtca tcgttgacta aaatcacgtt
    68041 tgccatctcc ggatcttgtc gctgttcaat gcaccacagc ctgaacgcct ctccctccag
    68101 taaagaaaac tcatcccgat gttttttcca ccagcttcgg cgcactctga caacgctcat
    68161 ttcccatgcg tgagcaccgg tggcataagg cttcaccaca ccgtcaatac aggtagccag
    68221 ccacagggaa acagactcct cagccagacg tgtcgacagt ttttccggga ggtaatcgtt
    68281 gatattggcg gcatagccgg gcctgaagtt cagcacaaac tttttagcca ttgcgcgatc
    68341 acagtaatat ttgcccactt gctcctgctc gctgcgggca aatccttccg gcattaccac
    68401 gtcctcaccg tagacggatt caataagaag ccgggctgcg tgtggcatct gaatagcacc
    68461 ttgctcacgc agcacacgct gcgtcagcca gattcgccca tgatcgggat agacaaatgc
    68521 actgttacgc atggcactgc caaaccattc gtcgccagga gagtcgtccc agacgggagc
    68581 cagaatcagc aattcagggg gggaacgctc gtcctttccg tcacgcttta actgaccatt
    68641 aatatcgcgg atatgccgct gtaatcgtcc tgctcgctga atcagcaaat caacaggggc
    68701 caggtcggag atcatttcgt ccaggtcaat atccacgctt tgctcaatta cttgtgtaca
    68761 aacaatgact tgcgacgctc tctgtaaact acaatattta ccaaagcgtg ccagcgtttc
    68821 cgtttcaatt cgctggcgat cgctaaaagc aaagcggcta tgaaaaagtg aaaggctgga
    68881 agcaggaatg acgccgcggg caagcagctg acgatgaacc ttaatagcgt catcgacaga
    68941 attccggatc caggcgatgc atttcccctg acttaccgcc gattcgatac gcgcaataca
    69001 ctcttgttca ctatgaagcc aacccacgct gacgctacgc tcaacgtctt tgcgcgtcgc
    69061 tacccggtgt gagttcacat cggatttcgt gacatgcgtc agccaggggt aatcatcctt
    69121 ttccaggaac ggagcttctt gctggccctc tgtaccccgc gcaaaggcag cgacgagttt
    69181 gtcgcgctgc tgttgagata acgtggcaga aagcaaaata acgctgtttc cgccacgcgc
    69241 ctgccgctcg atcaaccctt caagaatgca cgacatgtaa gcgtcacagg cgtggatctc
    69301 atcagccagc aggattttgt tactcagccc cagaagccgc agattattat gtttgaacgg
    69361 catcaccgcc atcatcgcct gatccagcgt gccgacgcca atttcagcca gcaacgcctt
    69421 cttgttactg tcggcaaacc aggccgcaca tccctgactg aacgtttgtt catccggttc
    69481 ttctgacccg actaaatcac cagaccagag tgattcatta aagcggtcca ttaatgtgcg
    69541 ggcactatgt gccagcacca ggctggggcg ggattctggc gaatagaaag caagccaggt
    69601 tttgaccagc cgatcgtaca tggcattggc cgttgccatt gttggcagac caaaaaacag
    69661 cccctgagct ttccccgcag ctatcaacct gtgcgccagg ataagcgccg cttctgtttt
    69721 acctgcgcca gtcacatctt ccagaataaa taactgcggt ccttgctggc tgatatccag
    69781 atccagcacc ttttgctgta acggtgtcgg attctcaata aaaggaaaca gcgtattaat
    69841 tccgttgaaa ggcgcgactt ttgcttttag aggaaagacg gttaaggcgt tttgagcctg
    69901 aattaaagtt ttctgccagt aacctttaat atccattggg tgtgcgacgc gtggaaaaaa
    69961 tcgcgttgac gaacccgtcc agtctgcgag cacgacggtt gcagagatat accaggaaag
    70021 gtgttttata agttcaatgc cctcatcatc atcccagaat gcggggatct ctatgagcgg
    70081 aaacagtggt ttgatttcaa ggagaaaatc tcgtgctgca gctttgtctt caggcagaaa
    70141 attatccaat tcatccatac gatcaggggg ccgaccatga tgccctgtcg tcacgggcat
    70201 ccacatttct atcactcgcc taagtttgcg cggagagagc gaagatgaag gaagtaactc
    70261 ctgacattca cttaaataat gattccacaa ccagtagccc agcgttgaat gagagatttt
    70321 ttcgtaattt tttctggcac cttcctgaat cttgagttca gggggcaggt aaagttgttg
    70381 aaaagagcgg gcaaattttc caatatcgtg ccagcacagc aaccaggcga aaaattgagc
    70441 cgcctgttcc ttgtcagaaa tccctaattg acgaaaataa tcagccagcc cgaagcaatt
    70501 tcttttaacc attaaatagc ccatcgcggc tacatcca
//
LOCUS       DAZKIV010000007        67907 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZKIV010000007 DAZKIV010000000
VERSION     DAZKIV010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.007"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            172..1107
                     /locus_tag="ACHOT6_000493"
     CDS             172..1107
                     /locus_tag="ACHOT6_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9945771.1"
                     /translation="MLTKRILFSVMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTL
                     HKMAESGDIDAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYD
                     GEQVRVDYAKAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIY
                     GGPEDFLATCRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGE
                     VANLRVTFSIRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFE
                     RKSCATVRTTIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(1163..1705)
                     /locus_tag="ACHOT6_000494"
     CDS             complement(1163..1705)
                     /locus_tag="ACHOT6_000494"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9945772.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1689..2000)
                     /locus_tag="ACHOT6_000495"
     CDS             complement(1689..2000)
                     /locus_tag="ACHOT6_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFP9945773.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2185..3075)
                     /gene="cyoE"
                     /locus_tag="ACHOT6_000496"
     CDS             complement(2185..3075)
                     /gene="cyoE"
                     /locus_tag="ACHOT6_000496"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFP9945774.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAEPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(3087..3416)
                     /gene="cyoD"
                     /locus_tag="ACHOT6_000497"
     CDS             complement(3087..3416)
                     /gene="cyoD"
                     /locus_tag="ACHOT6_000497"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFP9945775.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3416..4030)
                     /gene="cyoC"
                     /locus_tag="ACHOT6_000498"
     CDS             complement(3416..4030)
                     /gene="cyoC"
                     /locus_tag="ACHOT6_000498"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFP9945776.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(4020..6011)
                     /gene="cyoB"
                     /locus_tag="ACHOT6_000499"
     CDS             complement(4020..6011)
                     /gene="cyoB"
                     /locus_tag="ACHOT6_000499"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFP9945777.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKMNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(6033..6980)
                     /gene="cyoA"
                     /locus_tag="ACHOT6_000500"
     CDS             complement(6033..6980)
                     /gene="cyoA"
                     /locus_tag="ACHOT6_000500"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFP9945778.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7441..8916)
                     /gene="ampG"
                     /locus_tag="ACHOT6_000501"
     CDS             complement(7441..8916)
                     /gene="ampG"
                     /locus_tag="ACHOT6_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFP9945779.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8960..9538)
                     /gene="yajG"
                     /locus_tag="ACHOT6_000502"
     CDS             complement(8960..9538)
                     /gene="yajG"
                     /locus_tag="ACHOT6_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9945780.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9843..10160
                     /gene="bolA"
                     /locus_tag="ACHOT6_000503"
     CDS             9843..10160
                     /gene="bolA"
                     /locus_tag="ACHOT6_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFP9945781.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10504..11802
                     /gene="tig"
                     /locus_tag="ACHOT6_000504"
     CDS             10504..11802
                     /gene="tig"
                     /locus_tag="ACHOT6_000504"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9945782.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            12048..12671
                     /gene="clpP"
                     /locus_tag="ACHOT6_000505"
     CDS             12048..12671
                     /gene="clpP"
                     /locus_tag="ACHOT6_000505"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFP9945783.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12797..14071
                     /gene="clpX"
                     /locus_tag="ACHOT6_000506"
     CDS             12797..14071
                     /gene="clpX"
                     /locus_tag="ACHOT6_000506"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFP9945784.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14259..16613
                     /gene="lon"
                     /locus_tag="ACHOT6_000507"
     CDS             14259..16613
                     /gene="lon"
                     /locus_tag="ACHOT6_000507"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFP9945785.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLVLQNEPSGMQVVTAK"
     gene            16804..17094
                     /gene="hupB"
                     /locus_tag="ACHOT6_000508"
     CDS             16804..17094
                     /gene="hupB"
                     /locus_tag="ACHOT6_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFP9945786.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17286..19157
                     /gene="ppiD"
                     /locus_tag="ACHOT6_000509"
     CDS             17286..19157
                     /gene="ppiD"
                     /locus_tag="ACHOT6_000509"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9945787.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGYALEQQ"
     gene            19308..19679
                     /gene="ybaV"
                     /locus_tag="ACHOT6_000510"
     CDS             19308..19679
                     /gene="ybaV"
                     /locus_tag="ACHOT6_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFP9945788.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            19773..20171
                     /gene="fadM"
                     /locus_tag="ACHOT6_000511"
     CDS             19773..20171
                     /gene="fadM"
                     /locus_tag="ACHOT6_000511"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFP9945789.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20223..20918)
                     /gene="queC"
                     /locus_tag="ACHOT6_000512"
     CDS             complement(20223..20918)
                     /gene="queC"
                     /locus_tag="ACHOT6_000512"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFP9945790.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20983..22683)
                     /gene="ybaE"
                     /locus_tag="ACHOT6_000513"
     CDS             complement(20983..22683)
                     /gene="ybaE"
                     /locus_tag="ACHOT6_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HFP9945791.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKHIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTSEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22783..23601
                     /gene="cof"
                     /locus_tag="ACHOT6_000514"
     CDS             22783..23601
                     /gene="cof"
                     /locus_tag="ACHOT6_000514"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFP9945792.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23754..24212
                     /gene="decR"
                     /locus_tag="ACHOT6_000515"
     CDS             23754..24212
                     /gene="decR"
                     /locus_tag="ACHOT6_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFP9945793.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24242..26014
                     /gene="mdlA"
                     /locus_tag="ACHOT6_000516"
     CDS             24242..26014
                     /gene="mdlA"
                     /locus_tag="ACHOT6_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9945794.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     QQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            26007..27788
                     /gene="mdlB"
                     /locus_tag="ACHOT6_000517"
     CDS             26007..27788
                     /gene="mdlB"
                     /locus_tag="ACHOT6_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFP9945795.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27969..28307
                     /gene="glnK"
                     /locus_tag="ACHOT6_000518"
     CDS             27969..28307
                     /gene="glnK"
                     /locus_tag="ACHOT6_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFP9945796.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28337..29623
                     /gene="amtB"
                     /locus_tag="ACHOT6_000519"
     CDS             28337..29623
                     /gene="amtB"
                     /locus_tag="ACHOT6_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFP9945797.1"
                     /translation="MKIATIKTGLASLAMLPRLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29671..30531)
                     /gene="tesB"
                     /locus_tag="ACHOT6_000520"
     CDS             complement(29671..30531)
                     /gene="tesB"
                     /locus_tag="ACHOT6_000520"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFP9945798.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLHPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30749..31321
                     /gene="ybaY"
                     /locus_tag="ACHOT6_000521"
     CDS             30749..31321
                     /gene="ybaY"
                     /locus_tag="ACHOT6_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFP9945799.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31352..31663)
                     /locus_tag="ACHOT6_000522"
     CDS             complement(31352..31663)
                     /locus_tag="ACHOT6_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFP9945800.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31826..31922
                     /gene="ffs"
                     /locus_tag="ACHOT6_000523"
     ncRNA           31826..31922
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHOT6_000523"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            32042..32395
                     /gene="ybaA"
                     /locus_tag="ACHOT6_000524"
     CDS             32042..32395
                     /gene="ybaA"
                     /locus_tag="ACHOT6_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HFP9945801.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(32437..33987)
                     /gene="pdeB"
                     /locus_tag="ACHOT6_000525"
     CDS             complement(32437..33987)
                     /gene="pdeB"
                     /locus_tag="ACHOT6_000525"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFP9945802.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(34151..34621)
                     /gene="ylaC"
                     /locus_tag="ACHOT6_000526"
     CDS             complement(34151..34621)
                     /gene="ylaC"
                     /locus_tag="ACHOT6_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFP9945803.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(34737..35288)
                     /gene="maa"
                     /locus_tag="ACHOT6_000527"
     CDS             complement(34737..35288)
                     /gene="maa"
                     /locus_tag="ACHOT6_000527"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFP9945804.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTVTHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(35459..35677)
                     /gene="hha"
                     /locus_tag="ACHOT6_000528"
     CDS             complement(35459..35677)
                     /gene="hha"
                     /locus_tag="ACHOT6_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFP9945805.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(35703..36077)
                     /gene="tomB"
                     /locus_tag="ACHOT6_000529"
     CDS             complement(35703..36077)
                     /gene="tomB"
                     /locus_tag="ACHOT6_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFP9945806.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(36623..39772)
                     /gene="acrB"
                     /locus_tag="ACHOT6_000530"
     CDS             complement(36623..39772)
                     /gene="acrB"
                     /locus_tag="ACHOT6_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFP9945807.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(39795..40988)
                     /gene="acrA"
                     /locus_tag="ACHOT6_000531"
     CDS             complement(39795..40988)
                     /gene="acrA"
                     /locus_tag="ACHOT6_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFP9945808.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            41130..41777
                     /gene="acrR"
                     /locus_tag="ACHOT6_000532"
     CDS             41130..41777
                     /gene="acrR"
                     /locus_tag="ACHOT6_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFP9945809.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            41905..45267
                     /gene="mscK"
                     /locus_tag="ACHOT6_000533"
     CDS             41905..45267
                     /gene="mscK"
                     /locus_tag="ACHOT6_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFP9945810.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPDKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            45328..45426
                     /locus_tag="ACHOT6_000534"
                     /pseudo
     CDS             45328..45426
                     /locus_tag="ACHOT6_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45479..45640)
                     /gene="rsmS"
                     /locus_tag="ACHOT6_000535"
     CDS             complement(45479..45640)
                     /gene="rsmS"
                     /locus_tag="ACHOT6_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFP9945811.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(45654..46181)
                     /gene="priC"
                     /locus_tag="ACHOT6_000536"
     CDS             complement(45654..46181)
                     /gene="priC"
                     /locus_tag="ACHOT6_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFP9945812.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            46251..46628
                     /gene="ybaN"
                     /locus_tag="ACHOT6_000537"
     CDS             46251..46628
                     /gene="ybaN"
                     /locus_tag="ACHOT6_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFP9945813.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            46781..47332
                     /gene="apt"
                     /locus_tag="ACHOT6_000538"
     CDS             46781..47332
                     /gene="apt"
                     /locus_tag="ACHOT6_000538"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFP9945814.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            47461..49392
                     /gene="dnaX"
                     /locus_tag="ACHOT6_000539"
     CDS             47461..49392
                     /gene="dnaX"
                     /locus_tag="ACHOT6_000539"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFP9945815.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    48725..48789
                     /gene="dnaX"
                     /locus_tag="ACHOT6_000539"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            49445..49774
                     /gene="ybaB"
                     /locus_tag="ACHOT6_000540"
     CDS             49445..49774
                     /gene="ybaB"
                     /locus_tag="ACHOT6_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFP9945816.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            49774..50379
                     /gene="recR"
                     /locus_tag="ACHOT6_000541"
     CDS             49774..50379
                     /gene="recR"
                     /locus_tag="ACHOT6_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFP9945817.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            50489..52363
                     /gene="htpG"
                     /locus_tag="ACHOT6_000542"
     CDS             50489..52363
                     /gene="htpG"
                     /locus_tag="ACHOT6_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFP9945818.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            52544..53188
                     /gene="adk"
                     /locus_tag="ACHOT6_000543"
     CDS             52544..53188
                     /gene="adk"
                     /locus_tag="ACHOT6_000543"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFP9945819.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            53424..54386
                     /gene="hemH"
                     /locus_tag="ACHOT6_000544"
     CDS             53424..54386
                     /gene="hemH"
                     /locus_tag="ACHOT6_000544"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFP9945820.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(54383..55342)
                     /gene="aes"
                     /locus_tag="ACHOT6_000545"
     CDS             complement(54383..55342)
                     /gene="aes"
                     /locus_tag="ACHOT6_000545"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFP9945821.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            55494..56798
                     /gene="gsk"
                     /locus_tag="ACHOT6_000546"
     CDS             55494..56798
                     /gene="gsk"
                     /locus_tag="ACHOT6_000546"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFP9945822.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYEKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(56931..58607)
                     /gene="ybaL"
                     /locus_tag="ACHOT6_000547"
     CDS             complement(56931..58607)
                     /gene="ybaL"
                     /locus_tag="ACHOT6_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFP9945823.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(58845..60065)
                     /gene="fsr"
                     /locus_tag="ACHOT6_000548"
     CDS             complement(58845..60065)
                     /gene="fsr"
                     /locus_tag="ACHOT6_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFP9945824.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVELAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLAIFLPDNRHKD"
     gene            60283..61935
                     /gene="ushA"
                     /locus_tag="ACHOT6_000549"
     CDS             60283..61935
                     /gene="ushA"
                     /locus_tag="ACHOT6_000549"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFP9945825.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(61972..62451)
                     /gene="ybaK"
                     /locus_tag="ACHOT6_000550"
     CDS             complement(61972..62451)
                     /gene="ybaK"
                     /locus_tag="ACHOT6_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFP9945826.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(62655..63449)
                     /gene="ybaP"
                     /locus_tag="ACHOT6_000551"
     CDS             complement(62655..63449)
                     /gene="ybaP"
                     /locus_tag="ACHOT6_000551"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFP9945827.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            63587..63928
                     /gene="ybaQ"
                     /locus_tag="ACHOT6_000552"
     CDS             63587..63928
                     /gene="ybaQ"
                     /locus_tag="ACHOT6_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9945828.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(64143..66647)
                     /gene="copA"
                     /locus_tag="ACHOT6_000553"
     CDS             complement(64143..66647)
                     /gene="copA"
                     /locus_tag="ACHOT6_000553"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFP9945829.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            66909..67841
                     /gene="glsA"
                     /locus_tag="ACHOT6_000554"
     CDS             66909..67841
                     /gene="glsA"
                     /locus_tag="ACHOT6_000554"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFP9945830.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
BASE COUNT        17029 a        17126 c        17902 g        15850 t
ORIGIN      
        1 tatgctagtt ttatctcact aaataaacga tttggtgaca tggatgataa tgtttcaggc
       61 taaatgtgtg cttattactt ggacctttat ggcactgtat ttattcatta aatggatatt
      121 tggtggttaa ttattatgga aatatatgac agtgaggaca taaaggtata catgttaact
      181 aaaagaatat tattttctgt aatgctgata gtttcaccaa gcgtggtggc cagcgagaaa
      241 gcgcatgaat tgtatgacag catatacggt ggtaaacctg ctcccgatgt tataaataca
      301 ctgcataaaa tggctgaatc gggagatatc gacgcgcaga gcctgttagg ctgggaatat
      361 tatcagcctc gttatgatac caaacctgat gttcaggaag cgattaaatg gttcgagtta
      421 gcggctaaac aaggtgacag agaagctccg ttagcgctgg ggggtatcta ctacgacggt
      481 gagcaggtgc gggtggatta tgccaaagcg tatgcactat ttaatcaggc cgcgcagcac
      541 ggtgtgaatt tagcatggtc caggttgggc attatgtacg ccaatggtca gtatgttgag
      601 gtagattgta agaaagcgaa ggaatatctt gataaaggtg tccacattta tggtggcccg
      661 gaagactttc tggctacttg tcgaaaagac atgattgaca gaaaaacggt tgacgatacg
      721 ttacccgtga ttacggttac ccgttctggg atgcgagata attttttaga taaaggtttt
      781 tcctgcatgg atagcctctt cgccaccacc aataaattag gcgaagtggc caatcttcgc
      841 gtgacattca gcattcgtcg cccatccggt aaggagatta accaaacggt tggcttcgcg
      901 cctttcggtt taaaccggct gaatattagc tttactgatt acctatttgg ttcatttacc
      961 agtaattcgt ctcttattct ttataaaccg gaatttgagc gaaaatcttg tgcaactgta
     1021 aggaccacaa tcgttgccgc aacggcaacc attaacggca aggatgtgga gttgctgaaa
     1081 gcgggggcaa ttgaacaaaa gtggtaacgc tgctcagccc ccggctgagg caaaacttgc
     1141 cctggcgggt ggcgataata atctaagccg cgagaatatg ctgaatcgcc aaaaacatag
     1201 gtacagcacc aaaagcattt ggtgtcgctg aaagctgaac aaagccgaga cgagcataaa
     1261 aattaatggc ggcagggtca gcatcaagat aaaccccttt aattggtaat gcctggtgaa
     1321 ttatttttac atgttcaaaa aaatcacata gtaggtcctg accaaagccc tgcttttgat
     1381 acttccgtgc tacccccaac atgactaatc tgacaacacc aatttctgaa ggttgtgaac
     1441 cctgaaggac gccagaaacg cgttgttttt ccagcgaata tgccgtaaaa gtacagatgc
     1501 cgatcagttc accactttgt ctgtcaataa gtgctttagc cgcacagtcg ctattacgca
     1561 cacttttctt tagcgatgcg cgtacaaatt tatcgataac agggttaccg caatcaaact
     1621 gtttttgccc aggataagta atatcagact gataattaca ggagaggact atgggcagag
     1681 taatctcttc atgcttgtcg accatcgctg tttttccttt tcattaacgc cttgagggcg
     1741 agcgtcggtt gagcaggttc agcgatgagt tggttcatca gttcccagct ttgattcgaa
     1801 agctcacggc gacgttggtt atcaagcaca ctttcggcgc gttccatagc agcgctcaaa
     1861 ataaaagcgc ttaaatctac cccaacggct tgcgcagctt cacgaagttt gtttttggcg
     1921 tcgggactag ttttgagttc gacccgcgca tttttcagcg ttttatcgac atcgggtaga
     1981 gtggctgttt ttccaggcat tatgacctct ttgattaact gaatgaaatc gtagggagtc
     2041 gatctcatac ataatacggc tatcaaccgt atgattaatt tatgttcatc tactcactct
     2101 gtcaagacat acggttatta gccgtatctc tgatctcaga caataaaaaa ggtgccgtag
     2161 caccttttta atagagaggt tttgttacca cacagcagcc agcagcgtat gcgagtccgg
     2221 taccataaaa tcaacggaca tcatcaccga gagggcagtg atggcgatga tagagaagcc
     2281 gaacagcttg cgcgcccaga ttctgtcatc agcaacttta taaccgcgca gagccatacc
     2341 taaccaccag acgctaaccg ccgcggcgac caccagatat ttatatccag cgtaaccgcc
     2401 aagagagagc atcagcgtgg caacggcaaa ggcgatgata tacagcgtga tgtgattctt
     2461 cgccaccgaa atgcctttta ccactggcaa taccggaatg tttgccgcct ggtaatcctt
     2521 aaagcggaaa atggcgatgg cataggagtg aggcatctgc cacaggctga agatagccag
     2581 caggatcgct gcgccgctat cgaactcgcc ggttactgca cagtagccaa tcaccggcgg
     2641 ctcagcgccg gagagcgaac caatcaatgt gccgtagact gagtggcgtt tcatgtacag
     2701 gctatagacg ccgacataaa ccacaaagcc catcaccccc agccagcagg ccagcggatt
     2761 cgcgccaaac cacagcagca taaagccagc aaagcctagc aaggtggcgt acaccagcga
     2821 gacagcagga gagatcaggc ctttaaccag cacccgattc ttcgtccttt ccatctttct
     2881 gtcgatatcc ctgtcgatgt agttgttaaa cacacaaccc gacgccacaa ccagtgacac
     2941 cccaaccagc gtgtagataa acaggggata atcaatgctg ccctttgagg ccagcaggaa
     3001 tcccccaatc accgagatca ggttgccaaa gatgatgcct ggtttcgtta cttgcaggta
     3061 ttgcttaaac atcataaccg ccgctcttag tgcatcatca tgttgtagtt gaggttccac
     3121 ataatccaga tggagcctac aaccaggata gcgatgatta gcacggtgaa gacaaacgcc
     3181 gtcatgttcc agccttcatc tgatttggta ttcatgtgca ggaagcacac cagatgcacc
     3241 agaacctgta ccactgccat tgccaggatt gttcccagaa ttacggccgg agaggcagcc
     3301 cctgtcatca ccatccagaa cggaatcacc gtcagaatga tcgacaggat aaagcctgtc
     3361 atgtaggttt ttacgctgcc atgggacgcg ccgctgtgat cggtagaatg actcattaca
     3421 tcgcccccat cagataaaca acagtgaaca cacagatcca aaccacatcc aggaagtgcc
     3481 agaacaggct caggcacatg atgcgggtac ggttagtgct ggtcaggccg cgacgggcga
     3541 tttgcaccat cagcaccgcc atccagataa gaccagaagt gacgtgcaga ccgtgcgtgc
     3601 cgaccagcgc aaagaacgct gacaggaagc cgctgcgatc cggacccatg ccgttaacaa
     3661 tcaggtgatg gaattcatag atttccatcc cgataaatcc ggcaccaaac aaccaggtca
     3721 acgccagcca ggagataacc tggcttttgt tgtttttgta catggcgata gccgccatgc
     3781 cgtaggtgat ggagctgaac aacagcaaga aagtttcaac cagaacgaac ggcagttcga
     3841 aaatgtcctt acctgtcggg ccgcctgcgg tgccgttcac cagaacggca taggtagcaa
     3901 acaagattga gaacagaatg cagtcgctca tcaggtagat ccaaaatccg aagattttgg
     3961 ttccgcctgc atcgtggtgc ccgtgttcgt gcgcgtgggc agtcgcgtgc gtcaaagtat
     4021 cagttgccat ttttcagccc tgccttagta atctcatcga aatgctggtt ttccagtttt
     4081 tcgatttctg ccaccggcac gtagtaatcc acgtcctcgt cgaagctttt cacgatccag
     4141 gtgatgatca tgcctgcgaa gccaacaatc gccagccacc agatatgcca gatcatggcg
     4201 aaaccgaaga tggtggagaa agctgcaatg acgatacctg caccgctgtt tttcggcata
     4261 tgaatttctt catagtggtc aggcttttta tacgcttcgc ctttctcttt catttcccag
     4321 aatgcatcac gctcgtgaac gtgcggcaca acggcaaagt tatagaacgg aggcggggaa
     4381 gaggttgccc actccagcgt acggccaccc cacgggtcgc cagtcaggtc acggttctgg
     4441 tcgcggtcgc gaatagaaac gtacatctga ataacgaggc agagaatacc cagcgcaatc
     4501 agtactgcac cgctagctgc aatcatcagc atggtgtgga actgcgggtc aatctgctgg
     4561 ctcaaacgac gggtcatgcc catgaagccc agcgcataca gcggcataaa ggcaacgaag
     4621 aagccgatga tccagaacca gaacgcgcgt ttaccccagg tttcattcat tttgaaaccg
     4681 aacgctttag gccaccagta ggtcatccct gcgaagcagc cgaagaccac gccgccgatg
     4741 ataacgttat ggaagtgcgc aatcaggaac aggctgttat gcagaacgaa gtccgcgccc
     4801 ggtactgcca gcagcacgcc agtcatcccg cccaccgaga aagtgacgat aaaaccgatg
     4861 gtccacagca tcgcagaatg gaacacgatg cggccctgat acatggtgaa cagccagttg
     4921 aagatcttca ccccggtcgg gatggcgata atcattgtgg tgataccaaa gaaggcgttt
     4981 acgttcgcgc ccgcacccat cgtaaagaag tggtgcagcc aaacgatgaa cgacagcacg
     5041 gtgatacaga cggttgccca taccagcgag gtataaccaa acagacgttt acgcgagaag
     5101 gttgccgcaa tttcggagaa cacaccgaaa acaggcagga tcaggatgta aacttccggg
     5161 tggccccagg cccaaatcag gttgatgtac atcatcatgt tgccacccat atcgttggta
     5221 aagaaatggg tgcccagata gcgatccagg gtcaacaacg cgacggtaac cgtcagaatt
     5281 gggaaggaag caataatcag tacgttcgcg cacagtgatg cccaggtaaa tactggcatc
     5341 ttgaacatgg tcatgcccgg tgcgcgcatc ttcagaatgg taacgaagaa gttgataccg
     5401 gtaagcgtcg tacctatacc ggatagctgg agactccata tccagtaatc aacaccgact
     5461 cccggactgt actctattcc cgatagcggt ggataggcca gccagccggt ctgcgcaaat
     5521 tcgcccacgc cgagagaaac gttaaccaga atcacaccaa caacagtaaa ccagaagctt
     5581 aagttgttga ggaacgggaa cgcaacgtca cgcgcgccga tctgcagcgg aaccaccagg
     5641 ttcatcagac cgataacgaa aggcatcgcc acgaagaaga tcataatcac gccgtgcgcg
     5701 gtgaagatct gatcgtagtg gtgaggtggc aggaagcccg cttcacccgc cgaggcaaga
     5761 gcctgctggc tacgcatcat aatggcgtca gcaaaaccac gcagcaacat cacaatcgcc
     5821 acgatgatat acatgatacc gaggcgttta tggtcgacgg aggtcagcca ctctttccac
     5881 aggtaggtcc acttaccgaa gtaagtgatc aggccaacga gcgccagacc tcccaaaata
     5941 atgccagcga tcgtaaccat gacgataggt tcatggaacg ggactgcatc aagtgataat
     6001 tttccgaaca tctttattct tcctcaaccc ctttaatggg cggattccgc gtggctcatg
     6061 tccatgcctt ccataccttc gtgtgcactg tgctcacctt ctggctgggt catgtccatg
     6121 ctcttaccgt gagccataaa cttgttaatc acatcggcaa acaagtctgg tttcacgttg
     6181 gagaaatatt ccacctggtt gtattcgcta ggcgcggcca gtttttcgaa cgcagccatg
     6241 tcagacatgg tgttcggcga ctgtttcgct tttgcgaccc attggtcgaa ttcggcgcga
     6301 tccggtgttg caatagcttt gaacttcatg cctgagaagc ccggcccgct gtagctggcg
     6361 gagataccgt cataagtacc gggttcgttg gcgatcagat gcaggcgagt ctgcataccg
     6421 gccatggcat aaatctggct acccagacgc ggaatgaaga aggagttcat cacggagttg
     6481 gaggtcactt tgaagtacac cggagtgttc gccgggaaag cgatttcatt cacggtagca
     6541 atgccctgtt ccgggtagat gaagaaccat ttccagtcca tggaaaccac ttcgatggta
     6601 atgggcttct cgtcgtgtgc cagcggcttg ctaggctcaa gagcgtgagt ggttttccag
     6661 gtcagtaccg caaggaagat gatgattaag ataggtaccg tccagaccac agcttccact
     6721 ttattggagt gtgaccagtt cgggctgtac ttagcatctt tattgctcgc acgatacttc
     6781 caggcgaaac caacagccat caagattgcg ggaataacga caatcaacat caggccaaat
     6841 gccgtcagta tcagtgaacg ttgctccaga ccaatctgtc ctttgggatc taacagcgca
     6901 gaattacagc cactgagcaa tacagtgcct gcaaataatg acaaccatcc caaactttta
     6961 ttgtatttcc tgagtctcat ttaacgacct caattccacg ggatctggtg gcgtttaaag
     7021 tgtgtgggca ttttacggga aggttacatt actgtaaaca tgattaaatt cctgttactt
     7081 ggatttggct gtcaggtcac atatgttgca aaacatatca agttgatttt aagaagctgt
     7141 tgcaaaaggg cgttataacg aaaggggagt gcagaaggag tttggacggg caattggtat
     7201 aaccaatctg gaataaaaca attgtttaac aaataattat cattatggtg acaattgggt
     7261 ggggggaaaa taaagactca ttagctgaat cgtgtaagaa aaaatagaat tcataaattg
     7321 cgtcaataat ttccaaacgc taagccgcac aaaagaacaa atattattaa gcgatactga
     7381 tgtttattta gccgtaataa ttacggcgag tgattactac agctaaataa tatttacaga
     7441 ttacgtcaga tgcgtttttc gtagcgccag ataatccagc aaaccaccaa gcacgacgcc
     7501 agaaagggcg actaaaaccc cgacttccag cagagcaggc aggaaagaga agtgcgtcaa
     7561 atccagcgcg tccatcgtca acagcagtaa ccacacggcc aacaggctga cgcccgccgc
     7621 cagtgtccac atggcaaagg cataacctgc cggatattcg gtacgggaga taaagttgtc
     7681 atttactcgt gtatattcaa gcgtctggcg gcaaaccagc agcaaaataa gccccggtac
     7741 ggcagcggcg acggagaata gatagaatgt cgaccagccg tgtgcttcaa caaaccaacc
     7801 cgccacgggg ccgacataaa ctcgtcctac agcggaaagc gctgagagca gggcaaattg
     7861 agttgcggaa aatgacttat tacacagcgt cattaacagc gcgacaaagg ctgatgtgcc
     7921 catcccgcca cagaggtttt cgaaaaagac ggctgcgccc atgctgtaga gatgcttatc
     7981 agtaatcgac agcagccagt aaccagcgtt agacgcacct tgtaaaatgc cgaaaatcag
     8041 cagcgcccgg aacagcgaca ggcgctgcat caaaatccca ccgtacaatg cgcccacaat
     8101 ggtcgccagt aagccaagcg ttttgttaac cacgcctact tcacccgcat caaacccgac
     8161 gccgcgaatc aaaaacgtgg ttgtcaggct catggcgaat gcgtcgccca gcttatacag
     8221 cacgataaga agcaaaataa gccaggcatt attgcgacca aagaaatctc gcagaggtgc
     8281 aacaaccgct tgttccagcg tttttggcac aggaatggtg tcggtcggtt ctggtgcaag
     8341 caacgtcgcg ataatacaag ggatcaacag cgccgccatc aaccagtaca tgccctgcca
     8401 gcccagccat ttatctgcca gccacagggc taagccgccg gaaaccagca tccctaaacg
     8461 gtaacccagc acgctgattg ccgcgcccgc accacgttct tctgccggga gcacatcggt
     8521 tttccacgca tcgaagacaa tatcctggga ggccgagcaa aaagcgatca ccaccgccag
     8581 tgccgccatc cagcggagtt gggtgccagg ttcgagaaaa cccatcgccg caatggcgac
     8641 taataacagg atttgcgtgg cgagcagcca accgcgccgc cgcccaaaaa atgggggcgt
     8701 gtagcggtcc atcagcggtg accagaggaa tttaaaaacg taagcctggc ctaccagaga
     8761 gaagaaacca atggttttga gatcaatatt ctcgaccgtc atccaggcct gtaaggtgcc
     8821 ggatgtcagg gcgagcggta gcccggaagc aaaacccagg atcagcaata tggctgaacg
     8881 cggctgttga aaaatacgta aatattgact ggacatgggc gtctacaggc ccggcttgca
     8941 ccgggccaga gggcagaaat taacgcgcgt tctgcttgat gaattcgtga atgctggtgt
     9001 cctgagacat atcagcgatg gtatcggtca gcacactgtt aaccgcatcg gcgatatttt
     9061 tgttggaggc ctggaacgca ccttcaacgt tgtagctggc acgatagttt ttggtcattt
     9121 tgttgccatt ctgcgcggta gcgatgatgg cgatatccgc tttggtagcg atgttgtagc
     9181 gcacgttgcc ctgggacacg tcagcataca gttggctaac gatgatttgc agattaaccg
     9241 ggccattcgg accaaccatg taaccacgcg cggtcatctg tttttccagc acttcttgca
     9301 gcaggaaacg cagatcgcgg gaggcggtca gggtaacgat ttgattatcg cgggtgactt
     9361 ttgccagcgc ctgatcggta cgctgatcgg caccattaat gcttacggtg acgcccatca
     9421 ggcttggatc ctgctgtggc agtgtaatcg tcggggaaac ttcaatagtt gttggcggtt
     9481 ttgcgcatcc tgcaagcata aacagagcaa ctaacgggaa gaggattttt ttgaacatgt
     9541 tcgggctctc agagactctt aagcgtgttt ggtaaaaatt cccgccatca taacattgcc
     9601 aacggcgagg ggaagtgggt aaggcatgta aattcatcat gttgacgaaa taatcgcccc
     9661 tggcaaaaga aacactgatg cgaggcctgt gtttcaatct ttaaatcagt aaacttcata
     9721 cgcttgacgg aaaaaccagg acgaaaccta aatatttgtt gttaagctgc aatggaaacg
     9781 gtaaaagcgg ctagtattta aagggatgga tgacatctca gcgttgtcgg aggagatatt
     9841 tcatgatgat acgtgagcgg atagaagaaa aattaagggc ggcgttccaa cccgtattcc
     9901 tcgaagtagt ggatgaaagc tatcgtcaca atgtcccagc cggctctgaa agccatttta
     9961 aagttgtgct ggtcagcgat cgttttacgg gtgaacgttt tctgaatcgt catcgaatga
    10021 tttacagtac tttagcggag gaactctcta ctaccgttca tgcgctggct ctgcatactt
    10081 acactattaa ggagtgggaa gggttgcagg acaccgtctt tgcctctcct ccctgtcgtg
    10141 gagcaggaag catcgcgtaa aaacgcattt gcaactgtcg gcgcttttcc agtatgttgc
    10201 taaagatttt atgaaaaacg gcctgcgggc cgttttgttt tgtctggatt ttgcgctttt
    10261 tgcccagcat tcagacgaaa attgcccggg aattgtgaaa aaatacgcga cagcgcgcaa
    10321 taaccgttct cgactcataa aagtgatgcc gctataatgc cgcgtcctat ttgaatgctt
    10381 tcgggatgat tctggtaaca gggaatgtga ttgattataa gaacatcccg gttccgcgaa
    10441 gccaacaacc tgtgcttgcg gggtaagagt tgaccgagca ctgtgatttt ttgaggtaac
    10501 aagatgcaag tttcagttga aaccactcaa ggccttggcc gccgtgtaac gattactatc
    10561 gctgctgaca gcatcgagac cgctgttaaa agcgagctgg tcaacgttgc gaaaaaagta
    10621 cgtattgacg gcttccgcaa aggcaaagtg ccaatgaata tcgttgctca gcgttatggc
    10681 gcgtctgtac gccaggacgt tctgggtgac ctgatgagcc gtaacttcat tgacgccatc
    10741 attaaagaaa aaatcaatcc ggctggcgca ccgacttatg ttccgggcga atacaagctg
    10801 ggtgaagact tcacttactc tgtagagttt gaagtttatc cggaagttga actgcagggt
    10861 ctggaagcga tcgaagttga aaaaccgatc gttgaagtga ccgacgctga cgttgacggc
    10921 atgctggata ctctgcgtaa acagcaggcg acctggaaag aaaaagacgg cgctgttgaa
    10981 gcagaagacc gcgtaaccat cgacttcacc ggttctgtag acggcgaaga gttcgaaggc
    11041 ggtaaagcgt ctgatttcgt actggcgatg ggccagggtc gtatgatccc gggctttgaa
    11101 gacggtatca aaggccacaa agctggcgaa gagttcacca tcgacgtgac cttcccggaa
    11161 gaataccacg cagaaaacct gaaaggtaaa gcagcgaaat tcgctatcaa cctgaagaaa
    11221 gttgaagagc gtgaactgcc ggaactgact gcagaattca tcaaacgttt cggcgttgaa
    11281 gatggttccg tagaaggtct gcgcgctgaa gtgcgtaaaa acatggagcg cgagctgaag
    11341 agcgccatcc gtaaccgcgt taagtctcag gcgatcgaag gtctggtaaa agctaacgac
    11401 atcgacgtac cggctgcgct gattgacagc gaaatcgacg ttctgcgtcg tcaggctgca
    11461 cagcgtttcg gtggcaacga aaaacaagct ctggaactgc cgcgcgaact gttcgaagaa
    11521 caggctaaac gccgcgtagt tgttggcctg ctgctgggcg aagttatccg caccaacgag
    11581 ctgaaagctg acgaagagcg cgtgaaaggc ctgatcgaag agatggcttc tgcgtacgaa
    11641 gatccgaaag aagttatcga gttctacagc aaaaacaaag aactgatgga caacatgcgc
    11701 aatgttgctc tggaagaaca ggctgttgaa gctgtactgg cgaaagcgaa agtgactgaa
    11761 aaagaaacca ctttcaacga gctgatgaac cagcaggcgt aatttacgca gcataacgcg
    11821 ctaaattcgc acaaaggccc gtcaccgcca ggtggtgggc ttttttttgt catgaatttt
    11881 gcatggaacc gtgcgaaaag cctctttcgg tgttagcgta acaacaaaag attgttatgc
    11941 ttgaaatatg gtgatgccgt acccataaca cagggactag ctgataatcc gtccataagg
    12001 ttacaatcgg tacagcaggt tttttcaatt ttatccagga gacggaaatg tcatacagcg
    12061 gcgaacgaga taactttgca ccccatatgg cgctggtgcc gatggtcatt gaacagacct
    12121 cacgcggtga gcgctctttt gatatctatt ctcgtctact taaggaacgc gtcatttttc
    12181 tgactggcca ggttgaagac cacatggcta acctgattgt ggcgcagatg ctgttcctgg
    12241 aagcggaaaa cccagaaaaa gatatctatc tgtacattaa ctccccaggc ggggtgatca
    12301 ctgccgggat gtctatctat gacaccatgc agtttatcaa gcctgatgtc agcaccatct
    12361 gtatgggcca ggcggcctcg atgggcgctt tcttgttgac cgcaggggca aaaggtaaac
    12421 gtttttgcct gccgaattcg cgcgtgatga ttcaccaacc gttgggcggc taccagggcc
    12481 aggcgaccga tatcgaaatt catgcccgtg aaattctgaa agttaaaggg cgcatgaatg
    12541 aacttatggc gcttcatacg ggtcaatcat tagaacagat tgaacgtgat accgagcgcg
    12601 atcgcttcct ttccgcccct gaagcggtgg aatacggtct ggtcgattcg attctgaccc
    12661 atcgtaattg atgccagagg cgcaactgtg ccgctatact tatccagggc ggcacaacgc
    12721 tgtaagcggc ttgcgcctga gaatggcatt tgcgtcgtcg tgtgcggcac aaagaacaaa
    12781 gaagaggttt tgacccatga cagataaacg caaagatggc tcaggcaaat tgctgtattg
    12841 ctctttttgc ggcaaaagcc agcatgaagt gcgcaagctg attgccggtc catccgtgta
    12901 tatctgcgac gaatgtgttg atttatgtaa cgacatcatt cgcgaagaga ttaaagaagt
    12961 tgcaccgcat cgtgaacgca gtgcgctacc gacgccgcat gaaattcgca accacctgga
    13021 cgattacgtt atcggccagg aacaggcgaa aaaagtgctg gcggtcgcgg tatacaacca
    13081 ttacaaacgt ctgcgcaacg gcgataccag caatggtgtc gagttgggca aaagtaacat
    13141 tctgctgatc ggtccgaccg gttccggtaa aacgctgctg gctgaaacgc tggcgcgcct
    13201 gctggacgtc ccgttcacca tggccgacgc aaccacgctg accgaagccg gttatgtggg
    13261 cgaagacgtt gaaaacatca ttcagaagct gttgcagaag tgcgattacg acgtacagaa
    13321 agcgcagcgc gggattgtct acatcgatga aatcgacaag atttctcgta agtcagacaa
    13381 cccgtccatt acccgagacg tttccggtga aggcgtacag caggcactgt tgaaactgat
    13441 cgaaggtacg gtagctgctg ttccaccgca aggtgggcgt aaacatccgc agcaggaatt
    13501 cttgcaggtt gatacctcta agatcctgtt tatttgtggc ggtgcgtttg ccggtctgga
    13561 taaagtgatt tcccaccgtg tagaaaccgg ctccggcatt ggttttggcg cgacggtaaa
    13621 agcgaagtcc gacaaagcaa gcgaaggcga gctgctggcg caggttgaac cggaagatct
    13681 gatcaagttt ggtcttatcc ctgagtttat tggtcgtctg ccggttgtcg caacgttgaa
    13741 tgaactgagc gaagaagctc tgattcagat cctcaaagag ccgaaaaacg ccctgaccaa
    13801 gcagtatcag gcgctgttta atctggaagg cgtggatctg gaattccgtg acgaggcgct
    13861 ggatgctatc gctaagaaag cgatggcgcg taaaaccggt gcccgtggcc tgcgttccat
    13921 cgtagaagcc gcactgctcg ataccatgta cgatctgccg tccatggaag atgtcgaaaa
    13981 agtggttatc gacgagtcgg taattgatgg tcaaagcaaa ccgttgctga tttatggtaa
    14041 gccggaagcg caacaggcat ctggtgaata attaaccatt cccatacaat tagttaacca
    14101 aaaagggggg attttatctc ccctttaatt tttcctctat tctcggcgtt gaatgtgggg
    14161 gaaacatccc catatactga cgtacatgtt aatagatggc gtgaagcaca gtcgtgtcat
    14221 ctgattacct ggcggaaatt aaactaagag agagctctat gaatcctgag cgttctgaac
    14281 gcattgaaat ccccgtattg ccgctgcgcg atgtggtggt ttatccgcac atggtcatcc
    14341 ccttatttgt cgggcgggaa aaatctatcc gttgtctgga agcggcgatg gaccatgata
    14401 aaaaaattat gctggtcgcg cagaaagaag cttcaacgga tgagccgggt gtaaacgatc
    14461 ttttcaccgt cgggaccgtg gcctctatat tgcagatgct gaaactgcct gacggcaccg
    14521 tcaaagtgct ggtcgagggg ttacagcgcg cgcgtatttc tgcgctctct gacaatggcg
    14581 aacacttttc tgcgaaggcg gagtatctgg agtcgccgac cattgatgag cgggaacagg
    14641 aagtgctggt gcgtactgca atcagccagt tcgaaggcta catcaagctg aacaaaaaaa
    14701 tcccaccaga agtgctgacg tcgctgaata gcatcgacga tccggcgcgt ctggcggata
    14761 ccattgctgc acatatgccg ctgaaactgg ctgacaaaca gtccgttctg gagatgtccg
    14821 acgttaacga acgtctggaa tatctgatgg caatgatgga atcggaaatc gatctgctgc
    14881 aggttgagaa acgcattcgc aatcgcgtta aaaagcagat ggagaaatcc cagcgtgagt
    14941 actatctgaa cgagcaaatg aaagctattc agaaagaact cggtgaaatg gacgacgcgc
    15001 cggacgaaaa cgaagccctg aagcgcaaaa tcgacgcggc gaagatgccg aaagaggcaa
    15061 aagagaaagc ggaagcagag ttgcagaagc tgaaaatgat gtctccgatg tcggcagaag
    15121 cgaccgtagt gcgtggttat atcgactgga tggtacaggt accgtggaat gcgcgcagca
    15181 aggtcaaaaa agacctgcgt caggcgcagg aaatccttga taccgaccat tatggtctgg
    15241 agcgcgtgaa agatcgcatc cttgagtacc ttgcggttca aagccgtgtc aacaaaatca
    15301 agggaccgat cctctgcctg gtagggccgc cgggggtagg taaaacctct cttggtcagt
    15361 ccattgccaa agccaccggg cgtaaatatg tccgtatggc gctgggcggc gtgcgtgatg
    15421 aagcggaaat ccgtggtcac cgccgtactt acatcggttc tatgccgggt aaactgatcc
    15481 agaaaatggc gaaagtgggc gtgaaaaacc cgctgttcct gctcgatgag atcgacaaaa
    15541 tgtcttctga catgcgaggc gatccggcct ctgcactgct tgaagtgctg gatccagagc
    15601 agaacgtagc gttcagcgac cactacctgg aagtggatta cgatctcagc gacgtgatgt
    15661 ttgtcgcgac gtcgaactcc atgaacattc cggcaccgct gctggatcgt atggaagtga
    15721 ttcgcctctc cggttatacc gaagatgaaa aactgaacat cgccaaacgt cacctgctgc
    15781 cgaagcagat tgaacgtaat gcactgaaaa aaggtgagct gaccgtcgac gatagcgcca
    15841 ttatcggcat tattcgttac tacacccgtg aggcgggcgt gcgtggtctg gagcgtgaaa
    15901 tctccaaact gtgccgcaaa gcggttaagc agttactgct cgataagtca ttaaaacata
    15961 tcgaaattaa cggcgataac ctgcatgact acctcggtgt tcagcgtttc gactatggtc
    16021 gcgctgataa cgaaaaccgt gtcggtcagg taaccggtct ggcgtggacg gaagtgggcg
    16081 gtgacttgct gaccattgaa accgcatgtg ttccgggtaa aggcaaactg acctataccg
    16141 gttcgctcgg cgaagtgatg caggagtcta ttcaggcggc gttaacggtg gttcgtgcgc
    16201 gtgcggaaaa actggggatc aaccctgatt tttacgaaaa acgtgacatc cacgtccacg
    16261 taccggaagg tgcgacgccg aaagatggtc cgagtgccgg tattgctatg tgcaccgcgc
    16321 tggtttcttg cctgaccggt aacccggttc gtgccgatgt ggcaatgacc ggtgagatca
    16381 ctctgcgtgg tcaggtactg ccgatcggtg gtttgaaaga aaaactcctg gcagcgcatc
    16441 gcggcgggat taaaacagtg ctaattccgt tcgaaaataa acgcgatctg gaagagattc
    16501 ctgacaacgt aattgccgat ctggacattc atcctgtgaa gcgcattgag gaagttctga
    16561 ctctggtgct acaaaatgaa ccgtctggta tgcaggttgt gactgcaaaa tagtgacctc
    16621 gcgcaaaatg cactaataaa aacagggctg gcaggctaat tcgggcttgc cagccttttt
    16681 ttgtctcgct aagttagatg gcggatcggg cttgccctta ttaaggggtg ttgtaagggg
    16741 atggctggcc tgatataact gctgcgcgtt cgtaccttga aggattcaag tgcgatataa
    16801 attataaaga ggaagagaag agtgaataaa tctcaattga tcgacaagat tgctgcaggg
    16861 gctgatatct ctaaagctgc ggctggccgt gcgttagatg ctattattgc ttccgtaact
    16921 gaatctctga aagaagggga tgatgtagca ctggtaggtt ttggtacttt tgccgttaaa
    16981 gagcgtgctg cccgtactgg ccgcaacccg cagaccggta aagagatcac catcgctgct
    17041 gctaaagtac cgagcttccg tgcaggtaaa gcactgaaag acgcggtaaa ctaagcgttg
    17101 tccccggtgg ggatgtgacg aagttcaagg gcgcatctac tgatgtgcct tttttatttg
    17161 tattcggtga ctttctgcgt cttgtgggct gacaattgcc cccgtttctt gtcacaatag
    17221 gcctttgcgc gcatcgatac gttgcgtgag gtacacagtc atctacagcg gagtgttgtt
    17281 acaccatgat ggacagctta cgcacggctg caaacagtct cgtgctcaag attattttcg
    17341 gtatcattat cgtgtcgttc atattgaccg gcgtgagtgg ttacctgatt ggcggaggca
    17401 ataactacgc cgcaaaagtg aatgaccagg aaatcagccg tgggcagttc gagaacgcct
    17461 ttaacagcga gcgtaatcgc atgcagcaac agctgggcga tcaatactcc gagctggcag
    17521 cgaatgaagg ctatatgaaa accctgcgtc aacaggtgct gaatcgtctg atcgacgagg
    17581 cgctgttgga tcagtacgct cgtgagctga aactgggtat cagtgatgag caggttaaac
    17641 aggcgatttt cgcgacccca gccttccagg ttgacggcaa atttgataac agccgctata
    17701 acggtatcct caaccagatg gggatgaccg ccgatcagta cgcccaggcg ctgcgtaacc
    17761 agctcactac ccaacagctg attaacggtg ttgccggtac cgattttatg ctgaaaggtg
    17821 aaaccgacga gctggcggca ctggtcgcgc aacaacgcgt ggtgcgtgag gcgactatcg
    17881 atgttaacgc gctggcggcg aagcagcctg tgaccgaaca ggagattgcc agctactacg
    17941 aacaaaacaa aaacaatttc atgacgccgg aacaattccg cgtgagttac atcaagctgg
    18001 atgccgcaac gatgcagcaa ccggttagcg atgcggatat ccagagctac tacgatcagc
    18061 atcaggatca attcacccag ccgcagcgta cccgctacag catcatccag accaaaactg
    18121 aagatgaagc gaaagcggta cttgatgagc tgaataaagg cggtgatttt gctgcattag
    18181 ccaaagaaaa atctgccgat attatctctg cccgtaacgg cggcgatatg ggttggttag
    18241 aagatgccac tatccccgac gaactgaaaa atgctggtct gaaagaaaaa gggcaactgt
    18301 ctggtgtcat caaatcttcg gtcggtttcc tgattgtacg tctggacgac attcagccag
    18361 cgaaagtgaa atcgttagac gaagtacgtg acgacattgc ggcgaaagtg aaacacgaaa
    18421 aagccctcga tgcgtactat gcgctgcagc agaaagtgag cgatgcggca agcaacgaca
    18481 cggagtctct ggccggtgca gagcaagctg ccggcgttaa agccactcag acgggttggt
    18541 ttagcaaaga taacctgccg gaagagttga acttcaagcc ggttgccgac gctatcttta
    18601 acggcggtct ggtaggtgaa aacggcgcgc cgggcatcaa ctctgacatc atcaccgtag
    18661 acggcgaccg cgcattcgtg ctgcgcatca gcgagcacaa accggaagcg gtgaaaccgt
    18721 tggcagatgt tcaggaacaa gttaaggcac tggttcagca caacaaagct gaacaacagg
    18781 cgaaagtgga tgctgagaaa ctgctggttg atttgaaagc cggcaaaggt gcggaagcta
    18841 tgcaggctgc cggtctgaaa tttggcgagc cgaaaatctt aagccgttcc ggtcgtgacc
    18901 cgattagcca ggcggcgttt gcactgccac tgccagcgaa agacaaaccg agctacggta
    18961 tggcgaccga tatgcaaggt aatgtggttc tgctggcgct ggatgaagtg aaacaaggtt
    19021 caatgccgga agatcagaaa aaagcgatgg tgcagggtat cacccagaac aacgcacaaa
    19081 tcgtctttga agctctgatg agtaacctgc gtaaagaggc gaaaatcaaa attggctatg
    19141 cgctggaaca gcaataatcc tgaagccgcc tcgcaaaaaa atgcgttgca gctgtaacaa
    19201 gaaaaggtcg ctttcgcggc cttttccatt tctgaacatt gccatttgtt tactgttttc
    19261 actcccgtta aggtgattcc actgttaaca aacaaggaga aaacagtatg aaacacggaa
    19321 ttaaagcact gctcattacc ctgtccctgg cctgtgccgg aatgtctcat agcgcgctgg
    19381 cggcagcttc tgtggcgaaa ccgacggcgg tagaaaccaa agcggaagct cctgcagcac
    19441 aaagtaaagc agcagtaccg gcgaaagcca gtgacgaaga aggcacccgg gtcagcatta
    19501 ataatgccag cgcggaagag ctagcccgcg cgatgaatgg cgttggcctg aagaaagcgc
    19561 aggcgattgt cagttatcgc gaagagtacg gtccgtttaa aacggtggag gatctaaagc
    19621 aggtgccggg gatgggcaat tcgctggtgg aacgtaatct ggcggtatta accctgtaat
    19681 taatttgcat agtggcaatt ttttgccaga ctgaagaggt cataccagtt atgacctctg
    19741 tacttataac aacaacgtaa ggttattgcg ctatgcaaac acaaatcaaa gttcgtggat
    19801 atcatctcga cgtttaccag cacgtcaaca acgcccgcta ccttgaattt ctcgaagaag
    19861 cccgctggga tgggttggaa aatagcgaca gttttcagtg gatgacggcc cataacatcg
    19921 ccttcgtcgt ggtcaatatc aatattaact atcgtcgccc agcggtatta agcgacctgt
    19981 taactattac cagtcagttg cagcaattaa acggtaaaag cggcatctta agccaggtca
    20041 ttacactgga gccggaaggg caggtggtag cggatgcgct tattacgttt gtttgtattg
    20101 atcttaaaac gcagaaagca ttagctctgg aaggggaatt gcgcgaaaag ctggagcaga
    20161 tggttaagta aacgttttgt ggtgccggat gctcaagccg catccggcga cacccggaat
    20221 aattacctca acccggtttt ctgcttcatc gctgccatca ccgtcggttt atcggccaga
    20281 taatgattca aaccgttggc gcgtaaatta catgccgcac aatgaccgca accgtcgcct
    20341 ttaatgccgt tatagcaggt caacgtttcg ttacggacta aatccagttt gccgtaataa
    20401 tctgccagcg cccaggtttc cgctttatca atccacatca gcggcgtttc aaaacgaata
    20461 tctttcgcca tgcccaaact gacggcatgg tttagtgctt tcacaaactc atcgcggcaa
    20521 tccgggtagc cggagaaatc cgtttcacag acgccggtaa ttacggcttc tgcttttacc
    20581 tgatacgcat atattgccgc cagcgtcagg aacaaaatat tacgcccagg gacaaacgta
    20641 ttcgggatgc catcggcttc aggttcataa tcaggcaccg gaatgctgtc acgtgtcagg
    20701 ctgctgaccg ccagctcgtt gagcagcgtt acatcaagaa ccttatgcgc gcgtgctccc
    20761 agtttcagcg ccagttcgcg tgccacgtcg atttctgcgc gatgtcgctg accgtaatcg
    20821 aacgtcacgc aatggacttc atcatattgt tgtaatgcct gcaccagaca ggtggtggaa
    20881 tcctggcctc cactgaacac aacgacagca cgtttcatag ataatcctgc ttgaacaata
    20941 aaagcgttat ggtaacgcct gcgattaacc cggaccagct attcattgcg acggcgcggg
    21001 aagccaggct tcggtaaatt caaaccagcc gcgcggtgtc agtcgcacac cgttcacgcc
    21061 gggaggggca ctaatgcgat agtgatagtt gaacagcggc gtcagcgtcg cgtctgccat
    21121 taactggcta aaaaccgctt tcagggcatt aaatcggttt tcctcatcag gcattacttg
    21181 caccgcgtcc agcgtcgatt gcagatgtgc atatgctgga gcgtcgaaaa catgtggcca
    21241 tagtggatcg caacgtagcc attgctccag agtatattcc ggtgcttcgc caattaatct
    21301 gtcgcccatc atgaggtctg cgtgtgccag tagggtcgtg tcgtcccagt tttttgcgtt
    21361 atgaaaaata attgtgagtt cacagccttc cgctgccagt gtcgcctgta ggcgttctgc
    21421 catggtatga agttctatcg gtaggtgata aaccagcgtc aaggttttcg gtagtttgac
    21481 ttcatccggt acttgccagt gcggaatagt ccagcctggc agtaatgcat gactggcggt
    21541 gatcaggttt tctccgactt ctgacgtttg taataaaccg gattgatgaa taatggagat
    21601 cacttttcgc gcctgccaga gggagagtcg gggacttttg cgcaacgtta aatagcaaaa
    21661 acctaaactg atgccactac tgacctggct gacccgttgc agctcctccg gtttgccgat
    21721 ggtgatttgc acgggatgcc gacaactggt tcccaaatct ttttcgaaaa gcggcggagt
    21781 tatccagtac tcaaccgctt taagcagcgg atgacgcaaa tggtaataat catggctctc
    21841 caggcgcacc agctccgctg tgaattgtgt taagcgaaaa ggaccagtgc cgatcagtgg
    21901 gaattgcgga tgcgccagat ggctgcaata gctcgccagc cggtgcgcaa gccagtaatc
    21961 ggggcgatgt aaaaagaagg tcagacactg cggatgggtg acttcaatat gcttcacgct
    22021 aataaataat tgatccagtg ctggcagttg taacagcatc aataatcgct ggtgtaagtg
    22081 tgaggctttt actgcatcgc cgttatgcca gtgtagggtt gaacgaagat aaaagtccca
    22141 gcgtaacccg tcagtagagg tttcccagtg atgcgctaaa tcgccaatcg ggcgctgagt
    22201 attattatcg aagcgggtca ggccggaaaa tatctgcccg gcgagatgct gctcggcacg
    22261 gccgggcaaa aagcctggtt gtagcggttc gagcgggcga tagtagggaa tacgcaacgt
    22321 gggtgtatcg ttttgccatt gtccgcccat aaacggctgt aacagagtgc gcagctcacc
    22381 tggggccagt tgcgccagct ccagcacatc ttgctgcttt ccggtttcca gtgcctgttc
    22441 catcatcgca ttgcgtagcg attccggcgt gaccagaaag cgtaattgtc cgcgctttcc
    22501 gcgtcctgac tgcgcctgcc actccagcca tcccgcctcc tgtgcctgac gcaacagcgt
    22561 acgaacatgg cgttcgctgc aaaaacagcg ttcggccagt tcgctgacgg tgacggtttg
    22621 cggctttccg gctgaaggtt gccacagacg ttgatactgg ttaagacggt tgagcaatcg
    22681 catataaacc cggaacaata ttatttaact attcactatt acttccgtat atatcaggtg
    22741 atactcaatc accattaacc gtgtcacaga gtggagaaag aaatggctcg tctggcagca
    22801 tttgatatgg atggcacttt attgatgcct gaccatcatt taggtgagaa aaccctctct
    22861 actttggcgc gactgcgtga acgcgacatt accctcactt ttgccacggg gcgtcatgcg
    22921 ctggagatgc agcatattct cggggcgcta tcgctggatg catatttgat taccggcaac
    22981 ggaacgcgcg tgcattctct ggaaggtgaa cttttacatc gtgatgattt acctgcggat
    23041 gtcgcggagc tggtgctgta tcagcaatgg gatacccgag ccagcatgca tatcttcaat
    23101 gacgacggtt ggtttaccgg gaaagagatc cctgcgttgt tgcaggcatt tgtctatagc
    23161 ggttttcgtt atcagataat cgatgtcaaa aaaatgccac tcggcagcgt caccaagatc
    23221 tgcttctgtg gcgatcacga cgatcttaca cgcttgcaga tccagctata cgaagcatta
    23281 ggcgagcgtg cacatttgtg tttttccgcc acggattgcc tcgaagtgct gccggtgggc
    23341 tgcaataaag gcgctgcatt gacggtgctg acccaacatt taggtttatc gttgcgcgat
    23401 tgcatggcct ttggtgatgc gatgaacgat cgcgaaatgt taggcagcgt cggtagcggg
    23461 tttattatgg gcaatgcgat gccgcaactg cgcgcggagc tcccgcattt accggtgatt
    23521 ggacattgcc gaaatcaggc tgtctctcac tatttgacgc actggctgga ctatccacat
    23581 ctaccttatt cccccgaata acgagatccc ttccagcacc gggcaattgc ccggtttttt
    23641 ttgcgttgaa tttgtcattt tgtgccgtgg tgtttaaacc gcacagaata aattgtcgtg
    23701 atttcacctt taaaatagaa ttaaaagaga aaaaaattct ctgtggaagg gctatgttag
    23761 ataaaattga ccgtaagctg ctggccttac tgcagcagga ttgcaccctc tctttgcagg
    23821 cactggctga agccgttaat ctgacaacca ccccttgctg gaagcgcctg aaacggctgg
    23881 aggacgacgg tatccttatc ggcaaagtcg ccctgctgga tccggaaaaa ataggcctcg
    23941 gcctgaccgc ttttgtgctg ataaaaacgc aacatcacag cagcgaatgg tattgccgct
    24001 ttgtcacggt ggttaccgaa atgccagaag tgctggggtt ctggcgcatg gctggtgaat
    24061 acgattatct gatgcgcgtc caggttgccg acatgaaacg ctacgacgag ttttataagc
    24121 gtctggtaaa cagcgtgccg gggctgtcgg acgtcacttc cagcttcgcg atggaacaga
    24181 ttaaatacac cacttcttta cccatcgaat aaatatccag aatcaggtca ggacacaacg
    24241 cgtgcgatta tttgctcaat taagctggta tttccgtcgg gaatggcgtc gctatctcgg
    24301 ggctgtcgcc ttgcttgtca ttatcgcgat gctgcaactg gttccgccaa aagtggttgg
    24361 tattgttgtc gatggcgtga cagaacaaca ctttactacc gggcagatcc tgatgtggat
    24421 cgccaccatg gtgctgattg ccgttgtggt ttatctcctg cgttacgtct ggcgggtatt
    24481 gctgtttggt gcgtcttatc aactggctgt tgaactgcgt gaagattatt accgtcagct
    24541 aagccggcag catcctgagt tttacctgcg tcatcgcacg ggtgatctca tggctcgtgc
    24601 gaccaatgac gtcgatcgcg tcgtgtttgc cgccggagaa ggggtgctga cgctggtgga
    24661 ttcactggtg atgggctgcg ctgtgttgat tatgatgtct acgcaaatta gctggcagtt
    24721 gaccttattt gccctgttgc cgatgccagt gatggcgatc atgatcaagc gcaacggcga
    24781 tgccttgcat gaacgcttta agctggcaca ggcggcgttt tccagtctta atgaccgcac
    24841 ccaggaaagc ctcaccagta tccgcatgat caaagccttt ggtctggaag atcgccagtc
    24901 ggcgttattt gccgcggatg ccgaagatac cggcaaaaaa aacatgcggg tggcgcgtat
    24961 tgatgctcgt ttcgacccga caatctatat cgcgattggt atggcgaact tgctggcgat
    25021 tggcggcggt agttggatgg tggtgcaggg cagtttaacg ctgggccagc tcaccagttt
    25081 tatgatgtat ttaggtctga tgatttggcc aatgctggcg ctggcatgga tgtttaacat
    25141 tgtggaacgt ggtagtgctg cgtacagccg tattcgtgcg atgctggcgg aagcgccggt
    25201 ggtgaacgat ggtagcgaac ccgtgccgga agggcgtggc gaacttgatg taaatattca
    25261 ccagttcacg tatccgcaga ctgaccatcc tgcgctggaa aacgtcaatt tcgccctgaa
    25321 acccggtcag atgctgggta tctgcgggcc gactggttcc ggcaaaagta ccctgttgtc
    25381 gctcattcag cgtcatttcg acgtcagcga gggggatatt cgctttcatg atattcctct
    25441 gacgaagtta caactcgata gctggcgtag ccgcctggcg gtagttagcc agacaccgtt
    25501 ccttttttcc gacaccgtag cgaacaacat cgcgttgggt tgcccgaatg ccacccagca
    25561 agagattgag catgtcgcgc ggttagccag cgtacatgac gatattttgc gtttaccgca
    25621 aggttacgat acagaggtgg gcgagcgcgg cgtgatgctt tccggcgggc aaaaacagcg
    25681 tatctctatt gctcgtgcgt tattagtcaa cgcggaaatc ctcatccttg atgatgcgct
    25741 ttcggcggtg gacggacgca cagagcaaca gatcctgcat aacctgcgtc agtggggaca
    25801 gggaagaacg gtaatcatca gtgcccatcg cctttcggca ctgacggaag ccagtgaaat
    25861 tattgtgatg cagcacggac atatcgccca gcgtggcaat catgatgagc tggcgcaaca
    25921 aagcggctgg tatcgcgata tgtatcgcta tcaacaactg gaggcggcgc tcgacgacgc
    25981 tccggaaatt cgcgaggagg ccgtcgatgc gtagttttag ccaactgtgg ccgactctca
    26041 agcgtctgtt agcgtacggt tcgccgtggc gtaaaccgct ggggattgcg gtcctgatga
    26101 tgtgggttgc ggcagcggca gaagtcagtg ggccgctgct tatcagctat tttatcgaca
    26161 atatggtagc gaaaaataac ctgccgttga aaatggttgc agggctggct gcggcgtatg
    26221 ttgggctaca actgtttgcc gccgggctac attacgcgca gtcgctgctg tttaatcggg
    26281 cggcagtagg cgtagtgcaa cagttgcgta ccgacgtgat ggatgccgca ttgcgccagc
    26341 cattaagcga gtttgatacc caacccgtcg ggcaggtgat ttcccgcgtc actaatgaca
    26401 ctgaagtgat ccgcgatctc tacgttaccg tagtggcaac tgtcctgcgt agcgccgcac
    26461 tggtgggcgc gatgctggtg gcgatgttca gcctcgactg gcgaatggcg ctggtggcga
    26521 taatgatttt cccggtggtg ctggtggtaa tggtgatata ccagcgttac agcacgccga
    26581 ttgtccgtcg tgtgcgcgcc tatctagcgg atatcaacga cggctttaac gaaatcatca
    26641 atggcatgag cgttatccag cagttccgtc agcaggcgcg atttggcgaa cgtatggggg
    26701 aggccagtcg ttcacactat atggccagga tgcaaaccct gcgcctcgac ggttttctgc
    26761 tgcgtccgct gctgagtctg ttttcatcgc tcattctttg tggcttgttg atgctgtttg
    26821 gcttctccgc tagcggcacc attgaagtgg gcgtgctgta tgcgtttatc agctatcttg
    26881 ggcgacttaa cgaaccatta atcgaactga ccacgcaaca ggcgatgctg caacaggctg
    26941 ttgttgctgg tgagcgcgtg tttgaactga tggacggacc gcgccagcaa tatggcaatg
    27001 atgatcgccc gttacagagt ggcaccatcg aagtcgataa cgtgtcattt gcttatcgcg
    27061 atgacaatct ggtgctaaag aacattaatc tctctgtgcc ttcgcgtaac tttgtggcac
    27121 tggtcgggca taccggcagt ggcaaaagca ccctcgccag tttattgatg ggctattacc
    27181 cgctaacgga aggtgagatt cgccttgatg gtcgtccatt aagttcgcta agtcacagcg
    27241 cgctgcgcca gggcgtggca atggtgcagc aagatccggt ggtgctggcg gataccttcc
    27301 tcgccaacgt gacgctgggg cgtgatatct ccgaagaacg cgtctggcag gcgctggaaa
    27361 ccgtgcaact ggcggagctg gcgcgcagca tgagtgatgg tatttatacg ccgctgggcg
    27421 agcaggggaa taatctctcc gtcgggcaaa agcaactgct ggcactggca cgcgtgttgg
    27481 tggagacgcc gcaaatcctg atccttgatg aggcaaccgc cagcattgac tccggtactg
    27541 aacaggcgat tcaacatgct ctggcggctg tgcgtgaaca taccacgctg gtggtgattg
    27601 ctcaccgctt atcgaccatt gttgatgccg acaccattct ggtgcttcat cgtgggcaag
    27661 ccgtggagca gggcactcac cagcaactgc tggcggccca gggacgctac tggcagatgt
    27721 atcaactgca acttgcgggc gaagagctgg cagccagcgt gcgtgaagaa gaatcattga
    27781 gcgcctgaat agcgcaatat ttcatcgttg gtgcaaaaat gtaacgcact gtgcactgtc
    27841 atagtgcgtt ttcattttca atcttcttaa cttcctgctc tctttctcgt ttttcatttc
    27901 tggcacaccg cttgcaatac cttcttcgtg tagcagaacc attaccgaat tctgaccgga
    27961 ggggatctat gaagctggtg accgtgataa tcaaaccatt caagctggaa gacgttcgtg
    28021 aagcgttatc ttccattggt attcagggcc tgaccgtcac cgaagtgaaa ggtttcgggc
    28081 gtcagaaagg gcatgccgag ctgtaccggg gggcggaata cagcgtcaac ttcctgccaa
    28141 aagtaaaaat tgatgtggcg attgctgatg accaactcga tgaagtgatc gatatcgtca
    28201 gtaaggcggc ttacaccgga aaaattggcg acggcaaaat cttcgtcgct gaattgcaac
    28261 gcgtcattcg tattcgtacc ggcgaagccg acgaagcggc gctgtaatct ctggcacaca
    28321 gcaacaggaa cgaaaaatga agatagcgac gataaaaact gggcttgctt cactggcgat
    28381 gctgccgaga ctggtaatgg ctgcacctgc ggtggccgat aaagccgaca atgcgtttat
    28441 gatgatttgt actgcgctgg tgctgtttat gactattccg gggattgccc tgttttacgg
    28501 tgggttgatt cgcggcaaaa acgtgctgtc gatgctgacg caggtgacgg tgacgtttgc
    28561 actggtctgt attctctggg tggtttacgg ttactcgctg gcgtttggtg agggcaacaa
    28621 cttcttcggc aacattaact ggttgatgct aaaaaacatc gaactgacgg cggtgatggg
    28681 cagcatttat cagtatatcc acgtggcgtt tcagggctcg tttgcctgca ttaccgtcgg
    28741 cttgatcgtt ggggcgctgg cggaacgaat ccgcttctca gctgtgttga ttttcgtggt
    28801 ggtatggctg acgctctctt acattccgat tgcgcatatg gtgtggggcg gtggtttgct
    28861 ggcttctcac ggtgcgctgg atttcgcggg tggcacggtg gtgcatatta acgccgcaat
    28921 cgccggtctg gtgggcgcgt atctgatagg caaacgcgtg ggcttcggca aagaggcgtt
    28981 taaaccgcac aacctgccaa tggtcttcac cggtactgcc attctctata ttggttggtt
    29041 tggctttaac gccgggtcag cgggcacggc gaatgaaatc gcggcactgg catttgtgaa
    29101 tactgtggtc gcaacggcgg cggcaattct tggctggatc ttcggtgaat gggcgctgcg
    29161 tggtaagcct tctctgctgg gagcgtgttc tggcgcgatt gccggtctgg tcggcgtgac
    29221 gccagcgtgt ggctacattg gggttggcgg cgcgttgatt atcggcgtgg tagctggtct
    29281 ggcgggcttg tggggcgtta ctatgctcaa acgcttgctg cgggtggatg atccctgcga
    29341 tgtcttcggt gtgcacggcg tttgtggcat tgtcggctgt atcatgactg ggatttttgc
    29401 cgccagttcg ctgggtggcg tgggcttcgc tgaaggtgtg acgatgggcc atcagttgct
    29461 ggtacagctg gaaagcatcg ccattacgat cgtctggtcc ggtgtggtgg catttattgg
    29521 ctacaaactg gcggatctga cggttggtct gcgtgtaccg gaagagcagg agcgagaagg
    29581 gctggatgtc aacagtcacg gcgagaatgc ctataacgcg taacaagcac tgcaaaaaat
    29641 agccggacgg ttttacctcc ggctattttt ttaattgtga ttacgcatca ccccttcctg
    29701 aacggtcgag gcaaccagca cgccgtcctg ggtataaaac tcaccgcgca caaagccgcg
    29761 tgcgctggac gccgaggtgc tctccacgct atacagcagc cattcattca aattaaacgg
    29821 gcgatggaac cacatggaat ggtcaatggt ggcaatctga atccccggtt cgagaaaacc
    29881 gatgccgtgc ggctgtagag ctaccggcag gaagttaaga tcagaagcgt aaccgagcag
    29941 atactgatga acgcgcaggt catccggcac gctaccattt gcgcggatcc acacctgacg
    30001 atgtggttct gcgacgtgac ctttcagtgg gttatgaaac tccaccggac ggacttccag
    30061 cggacgatcg cagatgaatt tatctttaag cactggcggc agcaggtgcg ccagcgattg
    30121 ggcgatttgc gtttccgaag gcaggccatc aggcgctggc gcggacggca ttgttttttg
    30181 atgttcgaaa cccgcttctg gtgcctggaa agaggccgtc atataaaaaa tcggtttgcc
    30241 gttttgaata gcagcaaccc ggcgggcgct gaagctgtta ccgtcacgca gcgtttcgac
    30301 atcataaata atcggcttct tactatcgcc agggtgaaga aagtagctgt gaaacgaatg
    30361 taccagccgc tcttcaggga cggtctcttt tgcagcatac aaggcctgac ccacgacctg
    30421 gccgccaaac acctggcgta aacctaaatc ttcactctgg ccgcgaaaga gtccttcctc
    30481 aattttttcc agatttaaca atgtcagtaa attttttagc gcctgactca tataactctc
    30541 cagtaacaaa gctgccgcag caagccaaag tgagttgagt ataacgcaaa tttgctactg
    30601 gtccgatggg tgcaatggtc tgaattacgg gctaattaca ggcagaaatg cgtgatgtgt
    30661 gccacacttg ttgacgttac tattttgtta accactcttc cggcgaggaa agttagcccg
    30721 ctggtgcatt gataataagg agaaatgaat gaaactcgtg cacatggcca gtggtttagc
    30781 ggttgcgatt gcgttggcgg cttgcgcaga taaaagcgcg gatattcaga cgccagcccc
    30841 ggctgcaaat acgtctattt cagcaacaca acaacctgct atccagcaac cgaatgtctc
    30901 cggtaccgtc tggatccggc agaaagtcgc actgccgcct gatgctgtgc tgaccgtgac
    30961 actttctgac gcgtcgttag ccgatgcacc gtcaaaagtg ctggcgcaga aagcggtgcg
    31021 taccgaaggt aaacagtcac cattcagctt tgttctgcca tttaatccgg cagatgttca
    31081 gccgaacgcg cgtattctgt tgagtgcggc gattaccgtg aatgacaaac tggtatttat
    31141 taccgatacc gttcagccgg tgatcaacaa gggcggaact aaagccgacc tgacattggt
    31201 gccggtgcag caaaccgccg tgccggttca ggccagcggt ggtgcaacga ctaccgtacc
    31261 ttcgacttca ccaactcagg tgaatccgtc ttcggcagtt ccagctccta cgcaatatta
    31321 agcgacgtta accctctccg cccggagagg gttagtagtt ccagcgataa cgctgcaagt
    31381 cgatttgccc gcttcccgat accatcacac cttctgccag taatgcctgt cgctgacgct
    31441 gtaaatccgg tccggttagc gaaattgtgc cgtggcgatt aaccacccgg tgccagggta
    31501 aggtgctgcc ttcagggaga cgctttaaca caccgcccac ctggcgcgcg gcgcggggcg
    31561 atcccgccag tttcgccaca tcaccgtaag tggtgacata gccttcggga atagcggcga
    31621 cgatttgcca gacgcgttgg ggaaatgaat cttctttttc catcttttct tcctgaggta
    31681 atttttcagc ataatctgga aaaacgcccg agtgaagtcg cattgcgcaa gaaaccagca
    31741 tctggcacgc gatgggttgc aattagccgg ggcagcagtg ataatgcgcc tgcgcgttgg
    31801 ttctcaacgc tctcaatggg ggctctgttg gttctcccgc aacgctactc tgtttaccag
    31861 gtcaggtccg gaaggaagca gccaaggcag atgacgcgtg tgccgggatg tagctggcag
    31921 ggcccccacc catttctgcc tcccaccgtt tcgtcaaaaa accccaacat ggctaaactt
    31981 taaccacaac tgacgtcgca agaattgtct ggctgcgcag tacgcttcgg aggtatgtct
    32041 gatgaagtat gttgatggtt ttgtggttgc cgttcctgcc gataaaaagg atgcctatcg
    32101 ggaaatggcc gctaaggctg cgccattgtt taaagagttt ggcgcgcttc gtattgtcga
    32161 atgctgggcc agcgatgtac cggatggcaa agtgaccgat tttcgtatgg cggtgaaagc
    32221 ggaagagaat gaagaggttg tctttagctg gattgaatac ccttcaaaag aggtccgcga
    32281 cgctgctaat caaaagatga tgtcggaccc acggatgaaa gagttcggcg agtccatgcc
    32341 gtttgacggc aagcgaatga tctatggcgg attcgagtca atcatcgacg aataggtagc
    32401 gtgacgggct gtgcggcgtg ccgtcagccc aaaaattcac aaatgttgct cggcccagcg
    32461 taagaaatct tctttcggta atgccttgct gtagagccag ccctgaccgt agtgcacgcc
    32521 atgctggcgt agccactctt cttgtttact ggtttcgatt ccctccgcta ccattttcag
    32581 tttcagtgtt tttgccattt cgatgatatg cggcgtgaca tttttatatt ccagcgcatc
    32641 aacgaaagat ttatcgatct tcagaatgtc gacatccaga tcctgtaaat aacttaaact
    32701 tgaatatccc gtaccaaaat catcaagata aatttcatgg cccgcctccc ggtagcgaga
    32761 aattatcggg gcgctggttt tcggatcggc aaactcgcgt tcagtgagtt caagcgcgat
    32821 ctgtctggga ttaacctgat agtgattgat catttcacgc agcaattgcg ggattttttc
    32881 cgaggtgagc acggtggatt caagattgat cgaaatatgc tgctgtggat gctgacgcag
    32941 ccagtcgccc atatcttcaa agacgcttct tataatcagt agcgtcaatg gctcagaaag
    33001 gcccgtttgc tgtgccagcg gaataaaact atctggtgac aaccaactac cgtctgtctg
    33061 cggccagcgc gccagtgcct cagcaccgac aattttgcca ttggctaagg agacaatcgg
    33121 ctgatagtgc acgcaaatat cacgattttc gatagcatcc tgcagccgat gatgcggtga
    33181 ctggatacgg cgcaaaatac gcagcacaaa catcgctgcc agcaggccaa tcaccaaccc
    33241 gagcggtaac caaataaaga cttgccgatg ccagcctttc tgcaacattt tcgttgaagc
    33301 ccaggtgatg atcgaaatat tcatctccgg gaagggcagg atatcgtaaa tgattccatt
    33361 attttcgata tgctcaccgg gtgttttttg tagcctggta ataattccct gagcaatttc
    33421 atcgctgctg gttatgacaa cgttatgggc attgccaata atggcggcat caatttgcca
    33481 tgagctatag ggaatgacat caataaagga agcggggtcg atcatgacga cataatgtgc
    33541 cgttcccatg gcgaccatgt aacggataat gcctaaatcg ttatgcgatg ttaaccagac
    33601 acgataacca tctttcgaaa ttttacctgg ctcggggaag gtatcgggcg gactttcatg
    33661 ctccagagac gaacactggg gaacgttgtt atcgatataa accacttcct gaatatagcg
    33721 ataactgtaa gatacccgac gcatttccat gagatgggct tcgctacagg cagcgccttg
    33781 ccatctttcc agctcttgca gcgcatcttt cccttgtgtc gccaccttat tggcccgaat
    33841 agcgacgcgg gaggaatagg tatccagctc ttcaataaac gatgtttcta cctgctgatg
    33901 ggccagccag atgcttaagc cgacaggcag caatactgaa agaatcagta ctcccgaaat
    33961 aaggccgacc agatgtcgtg ttctcaccag catatcctta ttaacacagt gtagggaaag
    34021 tatatctgat tgtggaaaaa ggctggcagg agataaggat gtagagctgg tatttcatga
    34081 agtgatgggt ggaaacgatc attgtcattt gaccaggcgc agagctttac gcctggtatg
    34141 taaccctaac ttaagatgtt gattcggcgc gagcgagggt aaaaatatcg taaaaagaca
    34201 gttcaccttt acggacgatc attttttgca gttgttcctt atgaacatcc gcgacacgcg
    34261 gagagttcaa gatggcttca accagcgcgg caggtacaaa gcgcgtgttg taccattcgc
    34321 cgttatagca aacgcggaaa tccagcacgt cgatatcttc atagcggtag cgtgggtaga
    34381 catcgaaaaa gaagaaacca ttaagcagga taaataatac aaccagtagc cagacagagc
    34441 ctgacagcgt ttcggactgc aacatcaccg ccagggtggc aaaaaaagca acgtacatac
    34501 cgataaacag ccccggatgt ttacggataa aactgatact aaagcggggt ttgttgtcgc
    34561 gtttttcgcg ggtattcaga gactcaatcg tttcggtcag caggcgttgt atttcggtca
    34621 tcgtttgcct tcgtggtatc tgcaactttc cagggaacac cctattttaa cgtgggaata
    34681 gtagagggga agtaacagat ttaccacaat tttgcataac agttgcgaaa aaccgattac
    34741 aattttttaa ttattctggc tggattaccg cccacgacaa cgttgtccgg gacatctttt
    34801 gtgacaactg cacctgaggc taccacgacg ttatcaccaa tggtcacacc agggttaatg
    34861 accgcgcgtc cgccaatcca gacgttatta ccgatggtga cgggtttccc cagttcagca
    34921 ccgctattac gtgctacagg gtcgatggga tgtgttaccg tgtagatatg aacgcctggt
    34981 gccaacatac agttatcacc gatgcgaata gggcagacat caagcatcac gcaatcgaag
    35041 ttggcgaaaa aattattacc gagaaaaatg ttatagccat agtcacagcg aaacgttggc
    35101 tcaatataag cctctgtcac ctgaccgaat agatcagcga gaatttgctg gcgtaatgtg
    35161 tgctcttccg ccagggaatg attgtatcgg tgaataagct gacgagcgcg caggcgatcg
    35221 cgagataacg tctcatctgc cgagcgatac aactcaccag caatcatctt ttctttttct
    35281 gtgctcatta ctgacctccg gttgctgaaa atgcggcaac acagtaagac gaaaagtcag
    35341 tgaagagaac gttcccatta catctgtgtg ataaagatca catagggtat gtcttcatgg
    35401 tgaaaagtat aaaattctta ataaacagcc ggttatagct ccgaaagcga attatttatt
    35461 agcgaataaa tttccatact gaggaaggga tcttgtcgta tagtttattc atggtcaatt
    35521 cggcgaggcg gtgatctgcg gctgagtaaa ataccgccag ttcattatct gataattcgt
    35581 atttattttt ctcgataaca cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca
    35641 ttaaataatc ggttttcgtt aaaggttttt cggacatact tctacccatg gttgtaataa
    35701 ttctaacaag ataaactcgc aggattctct ttcgtcgcat tgacaaaaca acggaatagt
    35761 cgattacctg acttccgcca tttctgaaga tcctgcatat taataccata actgctgaac
    35821 aacataaagg tgtcatccag atattcgtcg atctgctcaa tgagcttatt gtcttcatta
    35881 tacttaattt tgtaattaag tgcgaaggtc gcaatatgct caatcagttc attcaactgg
    35941 aggttgatcg ccgaggttgg gtcgttaacc cagccatgat tgctttcttc aaggtttgca
    36001 agacagtcat gatacagggt ttcacagaga aacttaagct gtgcgatatc atgtctttta
    36061 ggtgagtatt catccataac gcgtcccctt cttagtggtt gaactaacgg acacctttcg
    36121 ggatggaaaa aacttactga cctggacttg ccctctcgtt tgttagctta actataagcc
    36181 actctttgca ggttttcatc gcataattac gaaaaattac aattaggaag gaatcgggac
    36241 aggactcact ttttcatact agcactaacc cgcagcaggt aaaagcagtt tgctcgaact
    36301 gttcataaca tttcaggatt atcgccagat gaaattggtg acactaatac caggattgct
    36361 ctgaatatga cgtaataacc gaggaatgaa taaagaatta ccgcaaataa ttaagaatag
    36421 cctctaaatg attatggatt attaaatcta ttagcgctac ttaatataaa ttaactaaca
    36481 atcagtattt tttatgaatt ttatccgtgg ttaatactgg ttttcgtatg agatcctgag
    36541 ttggtggttc aattactcct taatgttcgt aggttatgca taaaaaaggc cgcttgcacg
    36601 gccttagtga ttacacgttg tatcaatgat gatcgacagt atggctgtgc tcgatatctt
    36661 cattcttgcg gctaaagcgg cggcgaacca ccacaaagaa taccggaacg aagaagattg
    36721 ccagtaccgt tgcggtcacc atcccgccca ttacaccggt acctactgcg ttctgcgcgc
    36781 cggaaccagc accagtactg ataaccagcg gcataacgcc gaggataaac gccagcgagg
    36841 tcatcaggat cggacgtaaa cgcatccgca ctgcatcaag cgtcgcttca atcagacctt
    36901 taccttcttt atccatcaag tctttggcga attcgacgat aagtatcgcg ttcttcgccg
    36961 acaacccaat ggttgtgagc aggcctacct ggaagtaaac gtcattggtc aggccacgga
    37021 aggtggcagc cagcaacgca ccgataaccc ccagcggaac gaccagcata acggagaacg
    37081 gaatcgacca gctctcgtac agcgccgcca gacacaggaa tacgacaatc aacgaaatcg
    37141 cgtacagtga aggtgcctgg ttgccggaga ggcgctcctg ataggacatc cccgtccagt
    37201 catagccaac accggtaggc agtttgctcg ccagttgttc catcagctcc attgcttcac
    37261 cggtactttt acccggtgcc gcctggccta agatttccat ggatggcagg ccattgtaac
    37321 gttccagacg cggcgaaccg tactcccaac gagaagagga gaatgccgag aacggcacca
    37381 tctgaccatc agcagcacga acataccagt cgccgatatc atccggcagc atacggtatt
    37441 tcgcttctga catgacgtaa actttcttca cacgaccacg gtcgataaag tcgttcacat
    37501 agctaccgcc ccatgcagcg cccagagtgg tgttaatgtc attgatagaa acacccagcg
    37561 cctgcgcttt ttcctggtcg atatcaatct taaactgcgg ggtatcttcc agaccgtttg
    37621 gacgtacgct ggtcaacata tcagggtgct tcgctgcttc tgcaagcaac tggttacgcg
    37681 cctgagtcag tttttcgtga ccaaggccag cctggtcaat cagctcaaag tcaaagccgg
    37741 ttgcagtacc cagttccacg attgcgggca ggttaaaggc gaaaaccatc gcatctttga
    37801 tttgcgagaa agcgcgtgtt gcacgcatgg taatcgcttc aactttgttt tcttcgcccg
    37861 gacgatcggc ccagtccttc aaggaaacga acgcaatacc ggtattctga ccacgtcccg
    37921 caaagccgaa gccgttaacg gcgaacaccg actcaacgtt gttcttttct ttggtcagat
    37981 agtaatgcgt tacctcattg agcactttct gcgtacgttc ctgcgttgca cctgctggca
    38041 gctgaaccat ggtcataaat acgccctggt cctcatctgg caagaaggag cttggcagac
    38101 gcacgaacag ataggccatg ccgaccacga tgatcagata cagcaccagg taacgccccg
    38161 tactgcgcag aataccgcct acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc
    38221 ggttaaacca gccgaagaag ccttttttac cttccccgtg atcgcctttg gcaatcggtt
    38281 tcagcatggt ggcacaaaga gccggagtca ggatcaacgc taccagtacc gacagcgcca
    38341 ttgctgaaac aatggtaata gagaactgac gatagatagc accagtagaa ccgccaaaga
    38401 aggccatcgg tacgaatacc gctgacagta ccatcgcgat accgaccaga gcgccctgaa
    38461 tctgccccat cgacttacgg gtagcttctt ttggcggcaa accttcttcc gccataacac
    38521 gctccacgtt ttctaccaca acgatagcgt catccaccaa caagccgatg gcgagcacca
    38581 tcccgaacat tgttagcgtg tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc
    38641 cgagcaatac caccggtacg gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga
    38701 acagatacat aaccaggaac acgaggatga tcgcttcgac cagcgtttta accacttcgt
    38761 gaatagagat tttcacgaac ggcgtggtgt cgtacgggta aacaattttc agacccgacg
    38821 ggaagaacgg ttccatcttc gccagttcag cacggattgc cgcagcggta tccagcgcgt
    38881 ttgcaccggt cgccagcttg atccccagac cggaagccgg ttggccgtta aactctgcga
    38941 tgatgtcgta gttctcacca cccagctcaa tcttcgccac atcacgcagc agtacgcggg
    39001 aaccatcctg attcactttc agcaggattt tgccgaactc ttcagtagag gtcagacgcg
    39061 tctgagcaat aatagaggcg ttaagctgtt ggcctttcac cggcggcgta ccaccgagct
    39121 gacccgccgc aacctgggcg ttctgcgctt tgatggcggt aatgacatca accggcgtta
    39181 gctggaattt gttcagctca ttcgggttca tccagatacg catcgcgtac tgtgaaccga
    39241 acaactgaac atcacccacg cccgacgtac ggctgatggc atctttcata ttcgccgcta
    39301 cgtagtcaga gatatcctcc tgcgtcatgg tgccatcggt gttgataacg ccgacaacca
    39361 tcaggaagct gctggatgat ttctcaacgc tcaccccttg ctgctgaact tcttgcggca
    39421 gcaacggcat cgccagctgc agtttgttct gcacctgaac ctgcgcgata tccgcatcag
    39481 taccagactc aaaggtcagg gtgatctgca cggtacccgt ggagtcactg ttagaggaca
    39541 tgtacatcag gttatcgata ccgttcatat tctgttcgat aacctgtgtc accgtgtcct
    39601 gcactgtttt cgcatcagcg ccggggtagg aggcggagat cgttactgcc ggcggtgcaa
    39661 tcgtaggata ttgcgccacc ggcagtttga ggatcgccag cccccctgcc aacatgatga
    39721 taatggcgat cacccacgca aaaatcgggc gatcgataaa gaaattaggc atgtcttaac
    39781 ggctcctgtt taagttaaga cttggactgt tcaggctgag caccgcttgc ggcttgctgg
    39841 ttattatcag cggtaacttc ttgtgctttt acctggacac caggacgcac tttctgcagc
    39901 ccacttatta ctacgcgatc gcctgctttc agaccttcag tcaccagcca cttatcgcca
    39961 atagcctggc ttgcaacgat cggacgggtt tccactttgt catccgcgcc aactaccagt
    40021 acggtggcat caccacgcgg cgtacgggtt acgccctgtt gcgggactaa aatagcgttt
    40081 ggattaagcc cttcttccag acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc
    40141 gggttcggga agatagcgcg tagggtgata gacccagtgg tctgatcaac ggtaacgtca
    40201 gagaattcca gcgtaccgtc ctgcgggaac ttaatgccgt cactggtgat cagcgacact
    40261 ttggctttgc cgttctcttg tttcagcgtg ccattcgcca gttcctgttt caggcgcagg
    40321 aagtcgttgc tggactgggt cacatcaacg tagatcggat caagttgttg cacggttgcc
    40381 agcgcagtcg cctgaccgtt ctgtaccaat gcgccttccg tcacgttcga cttaccaatg
    40441 cgaccgctaa tcggagaggt gactttggtg taagccagat tgatccgcgc agtttcaacg
    40501 gcagctttcg ccgcagttac cgcagcattc gcctgttgcg catcagccag agcctgatcg
    40561 tactcttgct tactgatgta ctgagtaccg agcagtttct gataacgatt caccgtcaat
    40621 tgcgcgatat tggctgcagc ctgggctttc gccagatcac ctttcgcact gtcgtatgtc
    40681 gcctgatagg tcgcaggatc aatctgatag agagagacac cagcttcgat gtcgctacct
    40741 tctttgaaat tacgcttcag gataatcccg ctaacttgag gacgaacttc tgcgatccgg
    40801 taggcactgg tgcgacccgg aagctcggtt gtgatctgca gaggttcagt tttgactgtt
    40861 actacgccaa cggcgggcat ctgctggcca ccttgttggg cctgtttgtc gtcacatcct
    40921 gttagggcta agctgcctga gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt
    40981 ttgttcatat gtaaacctcg agtgtccgat ttcaaattgg tcaatggtca aaagttaata
    41041 aacccattgc tgcgtttata ttatcgtcgt gctatggtac atacattcac aaatgtatgt
    41101 aaatctaacg cctgtaaatt cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag
    41161 aaacgcgcca acacatcctc gatgtggctc tacgtctttt ctcacagcag ggggtatcat
    41221 ccacctcgct gggcgagatt gcaaaagcag ctggcgttac gcgcggtgca atctactggc
    41281 attttaaaga caagtcggat ttgttcagtg agatctggga actgtcagaa tccaatattg
    41341 gtgaactaga gcttgagtat caggcaaaat tccctggcga tccactctca gtattaagag
    41401 agatattaat tcatgttctt gaatccacgg tgacagaaga acgacgtcga ttattgatgg
    41461 agattatatt ccacaaatgc gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac
    41521 gtaatctctg tctggaaagt tatgaccgta tagaacaaac gttaaaacat tgtattgaag
    41581 cgaaaatgtt gcctgcggat ttaatgacgc gtcgggcagc aattattatg cgcggctata
    41641 tttccggcct gatggaaaac tggctctttg ccccgcaatc ttttgatctt aaaaaagaag
    41701 cccgcgatta cgtcgccatc ttactggaga tgtatctcct gtgccccacg cttcgtaatc
    41761 ctgccactaa cgaataaccc tgaatctgac tccaggattt ttcctggaca ttttcgtcgt
    41821 tgctattctg gttcactgcg tcgtgatatt cttgcggttt gactttttca ggtcgttctt
    41881 caggttcaga aaccttcatt catcatgact atgttccagt attacaaacg atcacggcat
    41941 tttgtttttt cagcatttat tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt
    42001 gcgcgggcgt catcgaatgg cgatctgccg acaaaagcgg acctgcaggc gcaacttgac
    42061 tcactaaata aacaaaaaga tctttctgct caggacaaac tggtgcagca ggatctgaca
    42121 gatacattag ccaccctcga taaaatcgat cgcataaaag aagagacagt tcagctacgg
    42181 caaaaagtcg ctgaagcgcc ggataaaatg cgccaggcga ccgcggcgtt aacagcactt
    42241 agcgatgtcg ataacgacga agaaacgcgc aaaattctga gtacgctttc gttgcgccag
    42301 ctggaaactc gcgttgccca ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg
    42361 tcttataaca gccagctggt ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg
    42421 tataacgctt cgcagcagct gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc
    42481 gagactgcct tacgtcccag ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat
    42541 gcggagattg accagcagcg taaaagcctg gaagggaaca ccgtcttgca ggataccttg
    42601 caaaagcaac gtgattacgt gacggcgaac agcgcccgtc tggagcacca gttgcagctg
    42661 ttgcaagaag cggtaaacag caagcgcctg actttaactg aaaaaacggc gcaggaagcc
    42721 gtctccccgg atgaagccgc gcgtattcag gctaatccgc tggtgaagca ggaactggaa
    42781 attaaccagc agttaagtca gcgtctgatt accgcgactg aaaacggtaa tcagttgatg
    42841 cagcaaaaca ttaaagtcaa aaactggctg gagcgggcgc tgcaatcgga acgcaatatt
    42901 aaagagcaga ttgccgtcct gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa
    42961 caacaaacgc tgccctcggc ggatgaactg gaaaacatga ccaaccgcat tgcggatttg
    43021 cgtctcgaac agtttgaagt taaccagcag cgtgatgcac tcttccagag cgatgcattc
    43081 gtcaacaaac tggaagaagg tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta
    43141 ttgcaagtgg ttgatatgcg tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac
    43201 cagctgatga tggccattaa cctgcaaatc aaccagcagc agttaatgag tgtgtcgaaa
    43261 aacctgaaat ccatcctgac tcagcaaatc ttttgggtga acagtaaccg tccaatggac
    43321 tgggactgga tcaaagcgtt cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc
    43381 acggtgaact gggaaaaagc ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg
    43441 ctgctgttga ttgccgggct gatccactgg cgtctgggct ggctgaaagc gtatcaacag
    43501 aaactggctt ccgctgtggg ttccctgcgt aacgatagcc agctcaacac accaaaagcg
    43561 atccttatcg acctgatccg cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg
    43621 attctgttga ccatgcagct caacatcagc gaactgctat ggtcgttcag caaaaaactg
    43681 gcgattttct ggctggtgtt tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc
    43741 gtgcgtcact tcggcatgcc ggagcagcag accagccact ggcgtcggca aattgtccgc
    43801 atcagtctcg cgttgctgcc tatccatttc tggtctgtgg tagcagaact ttccccgctg
    43861 catctgatgg atgatgtgct ggggcaggcg atgattttct tcaacctgct gctgattgcc
    43921 ttcctggtat ggccaatgtg ccgcgaaagc tggcgtgata aagagtcgca caccatgcga
    43981 ctggtcacca ttaccgtgct atcgattatc ccgattgcgt tgatggtgct gactgccacg
    44041 ggttatttct acaccacgct gcgcctctcc gggcgttgga ttgaaaccgt ttatttggtg
    44101 atcatctgga acctgctgta ccagacagta ttgcgcggct taagcgtagc ggcgcggcgt
    44161 atcgcctggc gtcgtgcgct ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt
    44221 gccgaaccgc cggaagaacc caccattgca ctggagcaag ttaaccagca gacgctgcgt
    44281 attaccatgt tgctgatgtt tgcgctgttc ggtgtcatgt tctgggcaat ttggtctgat
    44341 ttgatcaccg tgttcagcta tctcgacagc attacgctct ggcattacaa cggtactgaa
    44401 gctggtgctg cggtggtgaa aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc
    44461 tcaatggtgg cctgggcgtt gattcgcaac ctgcctggtt tactggaagt gctggtgctc
    44521 tcgcgactga atatgcgcca gggcgcgtcg tatgccatta ctaccatcct taactacatc
    44581 attattgccg ttggtgcgat gacggtgttc ggatcgctgg gcgtctcgtg ggataaactc
    44641 cagtggctgg cggcagcatt atccgtaggt cttggttttg gcttacagga gatcttcggt
    44701 aactttgtct ctggtttgat catcctgttc gaacgtccgg tgcgtattgg tgatacggta
    44761 accattggta gcttctcggg gacggtaagt aagatccgta ttcgtgcgac aacgattacc
    44821 gatttcgatc gcaaagaagt gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc
    44881 aactggtcgt tgactgacac tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc
    44941 tccgatctgg aaaaagtgcg taaagtgtta ctgaaggcgg cgactgagca cccaagggtg
    45001 atgcacgaac caatgccgga agtcttcttt acggcatttg gtgccagcac gttggatcat
    45061 gagctgcgtc tgtatgtgcg tgaactgcgt gaccgtagtc gtactgtcga tgagctgaac
    45121 cgtactatcg atcagctgtg ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa
    45181 gtgcatctgc acaacgagaa gggcgatgag gtgacggaag tcaaacgcga ctacaaaggc
    45241 gatgacccga cgccagcggt agggtaataa ccaaaggggg cgacatttag ttgccccgag
    45301 attactgaca aagtgcgcgt tgttcatgcc ggatgcggcg tgaccgcctt atccggccta
    45361 cgaaaccgca agaattcaat gtattgcagg agcggtgtag gcctgataag cgtagcgcat
    45421 caggcagttt tgcgtttgcc cacaatctta ggggacattt agcggcccca tttatttctc
    45481 acttttccgc ctcatcatcg cgcgttaatt tcttttcata atcgcgtttt acaatatcca
    45541 gcgcgcgcag cacggtgcgg gctgggatct gattttcttc cagcagcaca atcaaatcga
    45601 cagccagttt gacatcgtca ggggcatttt ccagtgacat actctctcca ttgctagcgg
    45661 gttaagcgcg ctagcctgtt ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc
    45721 gcttcatagg cttccacttc acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc
    45781 gccaggcggg ctctgcgttc ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc
    45841 tgaatacgtt tacgctgcca gcgggcaatt ttcggtggcg cactgtccca ctcgcgcaat
    45901 gaccaggcgg aggcttcacg cgcgatggct tccagttgtg ccgccagatg ttccgccagc
    45961 caggccactt gcggcagctg ttgctgctca actgcatgac gaagcgcagc cagattatcg
    46021 cccgcctcat cgagacaagc ctgcagtgtt gtcgcacggg tttgaaaaag gtgtctgtca
    46081 aaacgggcac ttagcgtggc aaattgcgct accggggcac aacgctgacg cagcgtagcg
    46141 agctgtcctt ccagtttttc cagcagcagg gcggttttca cgataagacc tcaatgaaaa
    46201 tgataattgt tatgctaaag tagccactct gtaagctgac aacactcaat atgcaacgaa
    46261 tcattttaat catcattggc tggctggcgg tagtgctggg tacgctgggc gtggtattac
    46321 cggtattacc gacgacgccg tttatcctgc tggcggcctg gtgctttgcc cgttcttccc
    46381 cgcgctttca cgcctggttg ctgtaccgct catggtttgg cagctatcta cgtttctggc
    46441 agaaacatca tgcgatgccg cgcggcgtca aaccgcgggc gattttgctt attttgctca
    46501 cgtttgccat ttctctgtgg ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa
    46561 ttctcgcctg tttgcttttc tatatgtggc gaattccggt gattgatgaa aagcaagaaa
    46621 agcactgaag cacaacaatc gcagttgcaa ttattgcgta cagccagtac attctggcgt
    46681 tttcgagcac aggcgcaggc ggtcaaaggt taaacaactg ttacttttga tacgtttaaa
    46741 acgcgccgtg agtaccaccg taacaagcag gcatacactt atgaccgcga ctgcacagca
    46801 gcttgagtat ctcaaaaata gcatcaaaag cattcaggac tacccaaaac ccggcattct
    46861 tttccgcgat gtcaccagct tactggaaga cccgaaagct tacgctctca gcatcgactt
    46921 gctggttgag cgttacaaaa atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg
    46981 cttcttgttt ggcgctccgg tagctctggg tctgggcgtt ggctttgtac cggtccgtaa
    47041 accgggcaaa ctgccgcgtg aaaccatcag tgaaacttac gacctggaat acggcaccga
    47101 tcagctggag atccacgttg atgccatcaa accgggcgac aaagttctgg tggtggacga
    47161 tctgctggca accggcggca ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg
    47221 tgaagtggct gacgctgcgt tcattatcaa cctgttcgat ctcggcggcg aacagcgtct
    47281 cgaaaaacag ggcattacca gctacagcct tgtcccgttc ccgggccatt aattatcgcc
    47341 agtcttgtgc tgcccacgct acggacagca caagatgtgc attcagcctc gccgttctga
    47401 cggggctgtg ttagcattac cccttcgtga atccaccttc cagcgtttca gagcctgcca
    47461 atgagttatc aggtcttagc ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc
    47521 caggaacatg tgctgaccgc actggcgaac ggcttgtcgt tagggcgtat tcatcatgct
    47581 tatctttttt ccggcacccg tggcgtcgga aaaacctcta tcgcccgact gctggcgaag
    47641 gggctaaact gcgaaaccgg cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt
    47701 gaaatcgagc aggggcgctt tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa
    47761 gttgaagata cccgcgacct gctggataac gtccagtacg ctccggcgcg tggtcgtttc
    47821 aaagtttatc tgatcgacga agtgcatatg ctgtcgcgcc acagctttaa cgcattgtta
    47881 aaaacccttg aagagccgcc ggagcacgtt aagtttctgc tggcgacgac cgatccacag
    47941 aaattgccgg tgacgatttt gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc
    48001 gagcaaattc gccatcagct tgagcacatc ctcaacgaag aacatatcgc tcacgagccg
    48061 cgggcgttgc aattgctggc gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg
    48121 accgaccagg cgattgccag cggtgacggc caggtttcaa cccaggcggt cagtgcgatg
    48181 ctgggtacgc ttgacgacga tcaggcgctg tcgctggttg aagcgatggt cgaggccaac
    48241 ggcgagcgcg taatggcgct gattaatgaa gccgctgccc gtggtatcga gtgggaagcg
    48301 ttgctggtgg aaatgctcgg cctgttgcat cgtattgcga tggtacaact ttcgcctgct
    48361 gcacttggca acgacatggc cgccattgag ctgcggatgc gtgaactggc gcgcaccata
    48421 ccgccgacgg atattcagct ttactatcag acgctgttga ttggtcgcaa agaattaccg
    48481 tatgcgccgg accgccgcat gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat
    48541 ccacgtatgc ctctgcctga gccagaagtg ccacgccagt cctttgcgcc cgtcgcgcca
    48601 acggcagtaa tgacgccaac ccaggtgccg ccgcaaccgc aatcagcgcc gcagcaggca
    48661 ccgactgtac cgctcccgga aaccaccagc caggtgctgg cggcgcgcca gcagttgcag
    48721 cgcgtgcagg gagcaaccaa agcaaaaaag agtgaaccgg cagccgctac ccgcgcgcgg
    48781 ccggtgaata acgctgcgct ggaaagactg gcttcggtca ccgatcgcgt tcaggcgcgt
    48841 ccggtgccat cggcgctgga aaaagcgcca gccaaaaaag aagcgtatcg ctggaaggct
    48901 accactccag tgatgcagca aaaagaagtg gtcgccacgc cgaaggcgct gaaaaaagcg
    48961 ctggaacatg aaaaaacgcc ggaactggcg gcgaagctgg cggcagaagc cattgagcgc
    49021 gacgcgtggg cggcacaggt gagtcaactt tcgctaccaa aactggtcga acaggtggcc
    49081 ttaaatgcct ggaaagagga gagcgacaac gcagtatgtc tgcatttgcg ctcctctcag
    49141 cggcatttga acaaccgcgg tgcacagcaa aaactggctg aagcgttgag cacgttaaaa
    49201 ggttcaacgg ttgaactgac tatcgttgaa gatgataatc ccgcggtgcg tacgccgctg
    49261 gagtggcgtc aggcgatata cgaagaaaaa cttgcgcagg cgcgcgagtc cattattgcg
    49321 gataataata ttcagaccct gcgtcggttc ttcgatgcgg agctggatga agaaagtatc
    49381 cgccccattt gatcgtaagc acagcttacg ttcgtcatcc ttaacgtgat tgagagagaa
    49441 acctatgttt ggtaaaggcg gtctgggtaa cctgatgaag caagcccagc agatgcaaga
    49501 aaaaatgcag aaaatgcagg aagagatcgc gcagctggaa gtcaccggcg aatctggcgc
    49561 aggtctggta aaagtgacca tcaacggtgc acacaactgc cgtcgcgtag agatcgaccc
    49621 gagcctgctg gaagacgaca aagagatgct ggaagacctg gtggctgcag cattcaacga
    49681 cgcagcacgt cgtattgaag aaacgcagaa agaaaaaatg gcctctgtat cctccggaat
    49741 gcagctgccg cctggcttta agatgccgtt ctgatgcaaa ccagcccgct gttaacacag
    49801 cttatggaag cactgcgctg tctgccgggc gttggcccga agtcggcgca gcgtatggcg
    49861 ttcacgctgc ttcagcgcga tcgtagcggc gggatgcgtc tggcgcaggc gctcacccgg
    49921 gcgatgtcgg aaatcggcca ctgcgccgat tgccgcactt tcaccgaaca ggaagtctgt
    49981 aacatctgtt cgaatccgcg tcgtcaggaa aacggtcaaa tctgtgtggt ggagagtccg
    50041 gcggacatct acgccattga gcagacgggg cagttttcag gtcgttattt tgtgttgatg
    50101 gggcatctgt caccgctgga cggcatcggt ccggatgata tcgggcttga tcgtctggaa
    50161 cagcgtctgg cagaggaaaa aatcactgaa gtgatcctcg ccaccaatcc cacggttgaa
    50221 ggtgaagcta ccgctaacta cattgccgag ctttgcgcgc aatatgacgt ggaagccagc
    50281 cgaatcgctc atggcgtacc ggttggcggc gaactggaaa tggtcgatgg caccacgctg
    50341 tcacactccc ttgccgggcg tcataagatt cgtttttaag caaacgagag caggatcacc
    50401 tgctctcgct tgaaattatt ctcccttgtc cccatctctc cctcatcctg tttttaacct
    50461 taaaatggca ttattgaggt agacctacat gaaaggacaa gaaactcgtg gttttcagtc
    50521 agaagtgaaa cagcttctgc acctgatgat ccattctctc tattccaata aagaaatctt
    50581 cctgcgtgag cttatctcta acgcctccga tgcggcggac aagctgcgtt tccgtgcgct
    50641 atctaacccg gacctgtacg aaggtgatgg cgaactgcgc gttcgtgtct ctttcgataa
    50701 agacaagcgc acgctgacca tctccgataa cggcgtgggg atgacccgcg acgaagtgat
    50761 tgaccatctg gggactatcg ctaaatccgg taccaaatca ttcctcgaat ccctgggttc
    50821 tgaccaggcg aaagacagcc agctgatcgg tcagtttggt gttggtttct actctgcgtt
    50881 tatcgtggcc gacaaagtga ccgtgcgtac tcgcgcggca ggcgaaaaac cagaaaacgg
    50941 cgtcttctgg gaatcggctg gcgaaggtga atataccgtt gccgacatca ccaaagaaga
    51001 tcgtggtact gaaatcaccc tgcatctgcg tgaaggcgaa gacgagttcc tcgatgactg
    51061 gcgcgtgcgt tccatcatca gcaaatactc cgaccatatc gcgctgccgg tagagatcga
    51121 aaaacgcgaa gagaaagacg gcgaaaccgt tatctcctgg gagaaaatca acaaagcgca
    51181 ggcgctgtgg actcgtaaca agtcggaaat caccgatgaa gagtacaaag agttctacaa
    51241 acacatcgcc cacgacttta atgatccgct gacctggagc cacaaccgtg ttgaaggtaa
    51301 gcaggagtac accagcctgc tgtacatccc gtcccaggct ccgtgggata tgtggaaccg
    51361 cgatcataaa cacggcctga aactgtatgt tcagcgtgtg ttcatcatgg acgacgcaga
    51421 acagttcatg ccgaactatc tgcgcttcgt gcgtggtctg attgactcca gcgatctgcc
    51481 gctgaacgtt tcccgtgaaa tcctccagga cagcacggta acgcgtaacc tgcgcaatgc
    51541 gctgaccaag cgtgtgctgc aaatgctgga aaaactggcg aaagacgacg cggaaaaata
    51601 ccagaccttc tggcaacagt ttggcctggt actgaaagaa ggtccggcgg aagatttcgc
    51661 taaccaggaa gcgatcgcca aactgctgcg ttttgcttct acttataccg attcttctgc
    51721 gcagaccgtg tctctggaag actacgtttc ccgcatgaaa gaagggcagg agaaaatcta
    51781 ctacatcact gcagacagct atgcggcagc gaagagcagc ccgcacctgg aactgctgcg
    51841 taagaaaggc atcgaagttc tgctgctttc cgaccgcatc gatgagtgga tgatgaacta
    51901 tctgactgag ttcgacggta aaccgttcca gtcggtgtct aaagttgacg agtcgctgga
    51961 aaaactggct gacgaagttg atgagagcgc gaaagaagcg gagaaagcac tgactccgtt
    52021 catcgaccgt gtgaaagccc tgctcggcga gcgcgtgaaa gatgtccgtc tgactcaccg
    52081 tctgaccgat acgccagcga tcgtttcgac cgacgcggac gaaatgagca ctcagatggc
    52141 gaaactgttc gctgcggcgg gccagaaagt gccagaagtg aaatacatct tcgaactgaa
    52201 cccggatcac gtactggtga aacgtgcggc agatactgaa gatgaagcga agttcagcga
    52261 gtgggtagaa ctgctgctgg atcaggcgct gctggcagaa cgcggcacgc tggaagatcc
    52321 gaacctgttt attcgtcgta tgaaccagct gctggtttcc tgatgtcatg ccggatgacc
    52381 tttgtgtcat ccggcatttt tcttttcatc atctgcactt tccgcaaatt atctcgccat
    52441 taaccgtttc agcctcaggt gcctttcttg aggcaatcgc ctgttggtgg tatcgtttat
    52501 cgctttttca aaaaattcga cacattttaa ggggattttc gcaatgcgta tcattctgct
    52561 tggcgctccg ggcgcgggga aagggactca ggctcagttc atcatggaga aatatggtat
    52621 tccgcaaatc tccactggcg atatgctgcg tgctgcggtc aaatctggct ccgagctggg
    52681 taaacaagca aaagacatta tggatgctgg caaactggtc accgacgaac tggtgatcgc
    52741 gctggttaaa gagcgcattg ctcaggaaga ctgccgtaat ggtttcctgt tggacggctt
    52801 cccgcgtacc attccgcagg cagacgcgat gaaagaagcg ggcatcaatg ttgattacgt
    52861 tctggaattc gacgtaccgg acgaactgat tgttgatcgt atcgtaggcc gccgcgttca
    52921 tgcgccgtct ggtcgtgttt atcacgttaa attcaatccg ccgaaagtag aaggcaaaga
    52981 tgacgttacc ggtgaagaac tgactacccg taaagacgat caggaagaaa ccgtgcgtaa
    53041 acgtctggtt gaataccatc agatgactgc accgctgatc ggctactact ccaaagaagc
    53101 ggaagcgggt aacaccaaat acgcgaaagt tgacggcacc aagccggttg ctgaagttcg
    53161 cgctgatctg gaaaaaatcc tcggctaatt cgaaagcgcg cacggacagt cccctcgccc
    53221 cctcggggag agggttaggg tgaggggaac aggcccgcac aagcaaactt atcagcaatc
    53281 tcaggccgga tattcattcg gccttttaca aaaacctcaa tctgcgctat tcctttttct
    53341 gatttgacct ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca
    53401 agttgaatcg ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc
    53461 tgggtacgcc cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa
    53521 gcgacagacg cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga
    53581 ttttgccgct gcgctcgccg cgtgtggcga agctgtacgc ctcggtctgg atggaagacg
    53641 gctcgccgct gatggtttac agccgccagc aacagcaggc gctggcacaa cgtttaccgg
    53701 agacgcccgt agcgctggga atgagctacg gctcgccatc actggaaagc gccgtcgatg
    53761 aactcctggc agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct
    53821 gttctacggt cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca
    53881 ttccggggat atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg
    53941 cgaacagcgt acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt
    54001 atcatggcat tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca
    54061 caacgactcg cgaactggct tccgcactgg ggatggcacc ggaaaaagtg atgatgacct
    54121 ttcagtcgcg ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa
    54181 tgctcggaga aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt
    54241 gtctggagac gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgccggcg
    54301 ggaaaaaata tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg
    54361 ctaatcttgt tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa
    54421 gagcctcgtc ggcggttttc atcattcgtg aataatgcaa aaaggcatgc agcgtacctg
    54481 ggtagagttt gaactcacag ggctgctgat gcgccgctaa cgtctggtaa agcagacggc
    54541 tgtcatccag cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag
    54601 tgagatcatt attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat
    54661 acgcctcttc atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca
    54721 gacgacgagt cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa
    54781 ttttaccgca atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg
    54841 cacctgcgga atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct
    54901 gctggtggaa ataacaacaa gcagccacaa tttcctctat cgcttgcgga aaacgcgctt
    54961 caggtgaaag ggggtaatca ataccaatca ccgtacattg gctgtagctt gccagcaggc
    55021 gcatgatgcg atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa
    55081 atagcgtcgc cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt
    55141 ttgttggaac catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc
    55201 gctcaagcgt gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta
    55261 aatccggctg aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag
    55321 gtagtttgtt ttccggcttc atacaaaaac tcctttccaa ttacgtcatt gtaaggaacc
    55381 actgccatga aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc
    55441 ccgtcagatt gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat
    55501 ttcccggtaa acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc
    55561 agcaattcca gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc
    55621 tggtcgatat tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg
    55681 ggcattcact ggtgattgag gatgacgtag ccgaagcgct ttatcaggag ctaaaacaga
    55741 aaaacctgat tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact
    55801 cggtgctcgc ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg
    55861 gcagttatgc ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac
    55921 aaggcgtgga tggcccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta
    55981 cctttgctat cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg
    56041 tgattgccgg agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg
    56101 aacccatgcc ggaagcaacc atgaaagcca ttgagtacga gaagaaatat aacgtaccgg
    56161 tggtgctgac gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat
    56221 tcctcaaaga ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg
    56281 gagaaagcga tccgttgttg gcatctgaca aggcgctgga ctgggtagat ctggtgctgt
    56341 gcaccgccgg gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta
    56401 aaacccagca tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc
    56461 gcgccatgcg ccacaaggat tgccagaatc cgctgcgtgt atattcgcac attgcgccgt
    56521 acatgggcgg gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag
    56581 cgttgctgca tgacattacc gccaacagct accatcgtag caacgtacca aactccagca
    56641 aacataaatt cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc
    56701 gtgtgagcta tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc
    56761 gtgaagacag cctggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca
    56821 gataaggcgt tcacgccgca tctggcattt ggctctcgat gcccgatgcg acgctggcgc
    56881 gtcttatcat gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc
    56941 accacttcac ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt
    57001 tcacccatca ctacctgatt cgcgccacgt tcggtgatat acgccacttc atcgtcataa
    57061 tgggcgcggg caataatctc aatatccgga tttttcgcgc gggcagatgc cacaatctca
    57121 cccgcttcat aaccgttggg aatcgtcagg atcagccatt ttgcacattc cagatgcgcc
    57181 agttgcataa tttcttcgtt cgccgcattg cccaatactg cgcggacccc gcgctctcgc
    57241 agctcatcaa cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc
    57301 aatttctccc ccagcaggct gcctacacga ccgtaaccca ccagtagcgc atggttgcaa
    57361 atatccactg ggatctgctt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc
    57421 gtttcggtct tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc
    57481 gacaggatcg cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc
    57541 attcccagtc ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg
    57601 gttaatgccg tacgttggga gtgaccaaac agtcgcacca ggaaaaatgc ggctaacgac
    57661 ttaccaaaca gaataatcgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt
    57721 aacggatcaa acaacatccc gacggagaca aaaaacagca ccgcaaacgc gtcgcgcaat
    57781 ggcagcgtat cgtgggcggc acggtgactc agttcagact cgttcagtac catcccggca
    57841 aagaacgcac cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct
    57901 aacgccagcg ccagcaccga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt
    57961 gccataatcc acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc
    58021 actttgccga tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt
    58081 tccatcattc ctgccactgc gggcagcaac accagcgtca gaaccattac caggtcttcc
    58141 acaatcaacc aaccgatggc gatttgccca cgctgactgt caattaattg ccgttcttca
    58201 agtgcgcgca gtaacaccac ggtactggcg gtggaaagac ataaaccgaa cacgataccg
    58261 gtcattaacg accagcccag cacggcagag agcgccatac ccagcagcgt cgccacggct
    58321 atctgggcga tcgcaccggg aatggcgatg gcctttaccg ccatcaaatc cttcagcgaa
    58381 aagtgcaaac cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca
    58441 agcttggtat cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga
    58501 tatcccacca gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc
    58561 acaaggccgc caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt
    58621 cctggtggtt attgtccatt tttggccggg aaaaccaaaa ttacaggtaa tagtttatga
    58681 caatttcatt gatgatgttc atgaataatt attgaatttt gcagaaaaat ggaattagct
    58741 gcaaaaaaag cacggatcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga
    58801 ctgtaaaacg atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg
    58861 ttatcaggca ggaatatggc caacatcccc aatagtggca ggaaagcaca gattttatag
    58921 actaactcga tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc
    58981 ataccaaaag caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagcagc
    59041 tcctgagcgt aaaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc
    59101 gttaaaaccc ccgtccagtg caggctggcg tagggtaaaa tcagcgtaaa cggcgcaacg
    59161 ccgaggatag agccccaaat cacatatttc cgcccaattt tatcccctac aggcccgccg
    59221 atcaccgtac ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg agcattctgg
    59281 atagataatc cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata
    59341 tagaaatatt tcgagaaaat gaggattaac agaatgctga ccgccagttc cactttgttg
    59401 cgcggcagtg gattgataat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc
    59461 gagtaccaac ggctgatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac
    59521 caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg ccgccagcaa gggtcccagg
    59581 gaactgccaa agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg
    59641 gaagccatac gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc
    59701 agcgccgccg ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca
    59761 cttaaggtaa agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag
    59821 tagccgacca ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca
    59881 atctgcataa atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc
    59941 gattggatca tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat
    60001 gttcgggcct tggtcgttga cgcagccgcg cccgccacag gctggggttg ttcactcatt
    60061 gccataggaa agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat
    60121 tacccgacta acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat
    60181 ttctaatctg gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgtcgcgtca
    60241 tactattttt caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg
    60301 ggcgtggcgt tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat
    60361 gagcaggata aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt
    60421 tggcgcaatg aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc
    60481 cgcaaagagg ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac
    60541 actggcgtgc ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg
    60601 gtgggctatg acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta
    60661 cgccagcagg aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt
    60721 actggcgagc gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc
    60781 gttattgggc tgacaaccga tgacacagca aaaattggta acccggaata cttcactgat
    60841 atcgaatttc gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca
    60901 gaaaagccag acattattat cgcggcgacc catatggggc attacgataa tggtgagcac
    60961 ggctctaacg caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg
    61021 atgatcgtcg gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag
    61081 gtcgattacg tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg
    61141 caggcgcatg agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa
    61201 atgaaaatgg ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa
    61261 gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc
    61321 tcgctgttat caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcgaa
    61381 accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg
    61441 cggttgattc tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga
    61501 ggcggaattc gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg
    61561 cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac
    61621 ctgaccgccg tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt
    61681 agctttgtgg cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat
    61741 ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat
    61801 ccgcgccttg ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg
    61861 aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag
    61921 gtgagctggc agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg
    61981 gcgggcgata tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc
    62041 gatatccagt ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc
    62101 gtcgataatc gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta
    62161 tcccgtcgaa cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc
    62221 ttttgctact tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg
    62281 tttcatatca ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc
    62341 taattttttg acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg
    62401 gatttgaaac gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt
    62461 cctgccttaa acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt
    62521 catcttgcct aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc
    62581 gcttaaagtg acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga
    62641 tattggccat ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt
    62701 gcaccgaccg cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg
    62761 agatttcgct gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt
    62821 atatcattat tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt
    62881 gcgttggtat gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc
    62941 tggagcaaca tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt
    63001 tgcttcgccg cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt
    63061 tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt
    63121 gagggagaaa tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta
    63181 atgcgctctt ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa
    63241 acatccgcct cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc
    63301 aggggagcca tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg
    63361 ccggggaggg tgatatcgat ggcaggccag gagtaatgat taccgcgcag cgcggcccaa
    63421 agcgttttta cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc
    63481 gcgcggggag ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca
    63541 gtatgttctt gtatcgctat tcacagggaa gcaacagtta aaaactatga aacaggcaac
    63601 aagaaaaccg acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt
    63661 gaaaatcaat gagttagcag agttgctgca tgttcatcgt aatagcgtca gtacactgat
    63721 caataataat cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac
    63781 cacagtcgat ttttggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa
    63841 catgcgcacc caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg
    63901 tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga
    63961 taaggcgttt acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacccgt
    64021 cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat
    64081 ccggcagtca tgcgtcgatg ccaaatgtgc caccctaaag cagcgcatcc gcaatgacat
    64141 acttattcct tcggtttaaa ccgcagcaac cggttggcgt tactcactac ggtaatcgac
    64201 gagagcgcca ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac
    64261 aaaataccgg cggcgaccgg aataccgata ctgttgtaga taaaggcacc gagcaggttc
    64321 tgcttcatgt tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg
    64381 ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc
    64441 gcaatgccga catccgcctg ggccagcgct ggcgcatcgt taatgccgtc gcccaccatt
    64501 gccacctgac gtccttcact ttgcaggcgt ttaatcgctt cggctttacc gtccggcagc
    64561 accccggcga tcacttcatc aatcccggct tctttggcga tcgcattggc ggtggttggg
    64621 ttatccccgg tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc
    64681 acgctatcac tacgcaatgg atcgcgtact gccagcaggg ctaccgcttt cccgtcaacc
    64741 gccagcagca caggcgttgc cccttgcgat gcctgagcag taatctccgc ttcgatagct
    64801 ttggtaccaa cctgttgctc atttaacagc gcctgattgc ccagcaataa cgcatgacct
    64861 tcagcttcac cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggcagc
    64921 tgcatatcac ctgctttatc gaggatcgcc cgtgccagcg gatggctgga accttgctcc
    64981 agtgccgccg ccagacgcaa tgcctgcgct tcatcaacat cagcaaacgt tttcactgcg
    65041 acaacctgcg gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt
    65101 gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc
    65161 ccgacgccgg aaataatcga catcggcgtc gccagcccca gcgcacacgg acaggcgata
    65221 atcagtaccg tggtggcaat caccagggta taaacaattt gcggtgccgg accaaagaag
    65281 taccagattg ccgcactgac cagcgcaatc accaccacca caggtacaaa tacggctgag
    65341 attttatccg ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga
    65401 atgatccgtg acagcgtggt atggctgcca accgcactgg cacgaaacag cacgctaccg
    65461 tcctgtacca ctgtcccggc atggacgctt tcgccttcgc ctttttgctg cgggattggt
    65521 tcgcccgtca gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc
    65581 ggcacgcgat cgccggtcgt caggcgcagc aacatacctg gctgcacttc cgccagaggc
    65641 acgctttttt caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat
    65701 ttctccagcg ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg
    65761 atcagaccga taatcatcgc gctggcttcg taataaagat gtcgcgcttc catcgggaac
    65821 cactgcggcc acaggttgac gctcatcgaa tagagccacg ccacgccagt acccagcgcc
    65881 accagcgtat ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa
    65941 tggccgccgg cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg
    66001 cggttgtcag cggtgaccat catgttatcg ccaatcatcc cccagaccat caccgggata
    66061 cccaccgcca gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc aacggcggtt
    66121 tcctgctggc gctcacggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct
    66181 ttttccaccg cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc
    66241 tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg
    66301 cgggtgacac agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca
    66361 tcggcggtcg ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat
    66421 gactccgcca gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg
    66481 atggtttcga tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac
    66541 acatccgctt gttccacatc cgggcgctgt tcgagacttt ctttcacgcg tttaacgcag
    66601 tgaccgcagg acaggccgtc cagggtcagg tcgatagttt gtgacataaa acactccttt
    66661 aagacagttt tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa
    66721 attaataaac caggcgagta aaagtccgta aagattaaaa aattggctca atttgcatca
    66781 ggattagaca tttatctctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca
    66841 ggatgcctca aaatcggcac cggggtgagg aattacctcc cgcatctata aaaaggagtt
    66901 aacaaaagat gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat
    66961 ttcactcact taacggcggt caaaatgccg actacattcc ctttctggcg aatgtgcctg
    67021 gtcaactggc ggcagtggct atcgtgacct gcgatggcaa ggtctatagt gcgggtgaca
    67081 gtgattaccg ctttgcgctg gaatccatct cgaaagtctg tacgttagcc cttgcgttag
    67141 aggatgtcgg cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct
    67201 ttaactcagt tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg
    67261 ctggcgccat tgccaccacc agcctgatta acgctgaaaa tgttgaacaa cgctggcagc
    67321 gaattttaca tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca
    67381 accagtcgga acaaacaacc aacttccata accgggccat tgcctggctg ctgtactccg
    67441 ccggatatct ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcca
    67501 cgctcctcaa tactgttgaa ctggcaacac ttggcgcgac gctggcggcg gggggagtga
    67561 atccgctgac gcataaacgc gttcttcagg ccgataacgt gccgtacatt ctggccgaaa
    67621 tgatgatgga agggctgtat ggtcgctccg gtgactgggc atatcgcgtt ggtttaccgg
    67681 gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc tggtgtgatg ggaattgccg
    67741 cgttctcacc accgctggac gaagaaggca acagtgttcg cggtcaaaaa atggtggcat
    67801 cggtcgctaa gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg
    67861 taataacggt aacaaacccc tcggtctatg gaacgtcgtt tccatcg
//
LOCUS       DAZKIV010000008        63419 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZKIV010000008 DAZKIV010000000
VERSION     DAZKIV010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 63419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63419
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.008"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            302..388
                     /gene="leuL"
                     /locus_tag="ACHOT6_000555"
     CDS             302..388
                     /gene="leuL"
                     /locus_tag="ACHOT6_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFP9945831.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            481..2052
                     /gene="leuA"
                     /locus_tag="ACHOT6_000556"
     CDS             481..2052
                     /gene="leuA"
                     /locus_tag="ACHOT6_000556"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFP9945832.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEIKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2052..3143
                     /gene="leuB"
                     /locus_tag="ACHOT6_000557"
     CDS             2052..3143
                     /gene="leuB"
                     /locus_tag="ACHOT6_000557"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFP9945833.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3146..4546
                     /gene="leuC"
                     /locus_tag="ACHOT6_000558"
     CDS             3146..4546
                     /gene="leuC"
                     /locus_tag="ACHOT6_000558"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFP9945834.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFDVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEDATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4557..5162
                     /gene="leuD"
                     /locus_tag="ACHOT6_000559"
     CDS             4557..5162
                     /gene="leuD"
                     /locus_tag="ACHOT6_000559"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFP9945835.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5211..6389)
                     /gene="setA"
                     /locus_tag="ACHOT6_000560"
     CDS             complement(5211..6389)
                     /gene="setA"
                     /locus_tag="ACHOT6_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFP9945836.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6491..6622)
                     /gene="sgrT"
                     /locus_tag="ACHOT6_000561"
     CDS             complement(6491..6622)
                     /gene="sgrT"
                     /locus_tag="ACHOT6_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFP9945837.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6711..8366
                     /gene="sgrR"
                     /locus_tag="ACHOT6_000562"
     CDS             6711..8366
                     /gene="sgrR"
                     /locus_tag="ACHOT6_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFP9945838.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGMESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEIDYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8400..8499
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8530..9513
                     /gene="thiB"
                     /locus_tag="ACHOT6_000563"
     CDS             8530..9513
                     /gene="thiB"
                     /locus_tag="ACHOT6_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFP9945839.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9489..11099
                     /gene="thiP"
                     /locus_tag="ACHOT6_000564"
     CDS             9489..11099
                     /gene="thiP"
                     /locus_tag="ACHOT6_000564"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFP9945840.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11083..11781
                     /gene="thiQ"
                     /locus_tag="ACHOT6_000565"
     CDS             11083..11781
                     /gene="thiQ"
                     /locus_tag="ACHOT6_000565"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFP9945841.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(11895..12659)
                     /gene="yabI"
                     /locus_tag="ACHOT6_000566"
     CDS             complement(11895..12659)
                     /gene="yabI"
                     /locus_tag="ACHOT6_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9945842.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12745..13623)
                     /gene="araC"
                     /locus_tag="ACHOT6_000567"
     CDS             complement(12745..13623)
                     /gene="araC"
                     /locus_tag="ACHOT6_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFP9945843.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13962..15662
                     /gene="araB"
                     /locus_tag="ACHOT6_000568"
     CDS             13962..15662
                     /gene="araB"
                     /locus_tag="ACHOT6_000568"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFP9945844.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDTAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15673..17175
                     /gene="araA"
                     /locus_tag="ACHOT6_000569"
     CDS             15673..17175
                     /gene="araA"
                     /locus_tag="ACHOT6_000569"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFP9945845.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17344..18039
                     /gene="araD"
                     /locus_tag="ACHOT6_000570"
     CDS             17344..18039
                     /gene="araD"
                     /locus_tag="ACHOT6_000570"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9945846.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18114..20465
                     /gene="polB"
                     /locus_tag="ACHOT6_000571"
     CDS             18114..20465
                     /gene="polB"
                     /locus_tag="ACHOT6_000571"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFP9945847.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIYHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20630..23536
                     /gene="rapA"
                     /locus_tag="ACHOT6_000572"
     CDS             20630..23536
                     /gene="rapA"
                     /locus_tag="ACHOT6_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFP9945848.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRKQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23548..24207
                     /gene="rluA"
                     /locus_tag="ACHOT6_000573"
     CDS             23548..24207
                     /gene="rluA"
                     /locus_tag="ACHOT6_000573"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFP9945849.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24324..25139)
                     /gene="djlA"
                     /locus_tag="ACHOT6_000574"
     CDS             complement(24324..25139)
                     /gene="djlA"
                     /locus_tag="ACHOT6_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFP9945850.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25394..27748
                     /gene="lptD"
                     /locus_tag="ACHOT6_000575"
     CDS             25394..27748
                     /gene="lptD"
                     /locus_tag="ACHOT6_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFP9945851.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWKGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27801..29087
                     /gene="surA"
                     /locus_tag="ACHOT6_000576"
     CDS             27801..29087
                     /gene="surA"
                     /locus_tag="ACHOT6_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFP9945852.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLITDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29087..30076
                     /gene="pdxA"
                     /locus_tag="ACHOT6_000577"
     CDS             29087..30076
                     /gene="pdxA"
                     /locus_tag="ACHOT6_000577"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFP9945853.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30073..30894
                     /gene="rsmA"
                     /locus_tag="ACHOT6_000578"
     CDS             30073..30894
                     /gene="rsmA"
                     /locus_tag="ACHOT6_000578"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFP9945854.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30897..31274
                     /gene="apaG"
                     /locus_tag="ACHOT6_000579"
     CDS             30897..31274
                     /gene="apaG"
                     /locus_tag="ACHOT6_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFP9945855.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31281..32129
                     /gene="apaH"
                     /locus_tag="ACHOT6_000580"
     CDS             31281..32129
                     /gene="apaH"
                     /locus_tag="ACHOT6_000580"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFP9945856.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     GEAVAS"
     gene            32154..32753
                     /locus_tag="ACHOT6_000581"
     CDS             32154..32753
                     /locus_tag="ACHOT6_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4291 domain-containing protein"
                     /protein_id="HFP9945857.1"
                     /translation="MADYFEIRADYNQHTITIYQAYNDAIADVAVRDGRFGAPFSFNR
                     MTWIKPSFMWMMERSNWGLKKDQQHILAIRIKRTFFDTLLEQAVLTTPEAHVYPHAGI
                     WETLFAQANVYVQWDPERSINGKKLEHRSLQLGISRNLISQFNEDAIVAIDDLTPLVR
                     KCHNLLINGKTTQAKSFLPPEKIYPVSAAARKALGMKNS"
     gene            32789..33256
                     /locus_tag="ACHOT6_000582"
     CDS             32789..33256
                     /locus_tag="ACHOT6_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9945858.1"
                     /translation="MRQINTGLNTREIILEMIDEADAPPFAEQRTWHRRRIAFLCDCI
                     SVAVDYGFFCSVSQQSIMNITKMAEDVWITESKTTQLIDIWQQLKQLYPPETIPYAQK
                     LPTKELLELLLSSPDDLRIIEIPDIIGDVFDDQTWIRLFKKHFADVIQLRCDV"
     gene            complement(33355..33534)
                     /locus_tag="ACHOT6_000583"
     CDS             complement(33355..33534)
                     /locus_tag="ACHOT6_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HFP9945859.1"
                     /translation="MNDKTFDFTSERDFVSRAHCVGLNLQATLQWNEVNEDAISALNE
                     FAEETGCFSDEYRKF"
     gene            complement(33555..34034)
                     /gene="folA"
                     /locus_tag="ACHOT6_000584"
     CDS             complement(33555..34034)
                     /gene="folA"
                     /locus_tag="ACHOT6_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFP9945860.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(34226..36088)
                     /gene="kefC"
                     /locus_tag="ACHOT6_000585"
     CDS             complement(34226..36088)
                     /gene="kefC"
                     /locus_tag="ACHOT6_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFP9945861.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(36081..36611)
                     /gene="kefF"
                     /locus_tag="ACHOT6_000586"
     CDS             complement(36081..36611)
                     /gene="kefF"
                     /locus_tag="ACHOT6_000586"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFP9945862.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(36719..38050)
                     /gene="yaaU"
                     /locus_tag="ACHOT6_000587"
     CDS             complement(36719..38050)
                     /gene="yaaU"
                     /locus_tag="ACHOT6_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9945863.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFEEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(38109..38396)
                     /gene="fixX"
                     /locus_tag="ACHOT6_000588"
     CDS             complement(38109..38396)
                     /gene="fixX"
                     /locus_tag="ACHOT6_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFP9945864.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKVCP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(38393..39679)
                     /gene="fixC"
                     /locus_tag="ACHOT6_000589"
     CDS             complement(38393..39679)
                     /gene="fixC"
                     /locus_tag="ACHOT6_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9945865.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(39730..40671)
                     /gene="fixB"
                     /locus_tag="ACHOT6_000590"
     CDS             complement(39730..40671)
                     /gene="fixB"
                     /locus_tag="ACHOT6_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9945866.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(40686..41456)
                     /gene="fixA"
                     /locus_tag="ACHOT6_000591"
     CDS             complement(40686..41456)
                     /gene="fixA"
                     /locus_tag="ACHOT6_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFP9945867.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            41930..43444
                     /gene="caiT"
                     /locus_tag="ACHOT6_000592"
     CDS             41930..43444
                     /gene="caiT"
                     /locus_tag="ACHOT6_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFP9945868.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            43475..44617
                     /gene="caiA"
                     /locus_tag="ACHOT6_000593"
     CDS             43475..44617
                     /gene="caiA"
                     /locus_tag="ACHOT6_000593"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFP9945869.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            44746..45963
                     /gene="caiB"
                     /locus_tag="ACHOT6_000594"
     CDS             44746..45963
                     /gene="caiB"
                     /locus_tag="ACHOT6_000594"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFP9945870.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKAPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            46037..47590
                     /gene="caiC"
                     /locus_tag="ACHOT6_000595"
     CDS             46037..47590
                     /gene="caiC"
                     /locus_tag="ACHOT6_000595"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HFP9945871.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            47699..48484
                     /gene="caiD"
                     /locus_tag="ACHOT6_000596"
     CDS             47699..48484
                     /gene="caiD"
                     /locus_tag="ACHOT6_000596"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFP9945872.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            48490..49080
                     /gene="caiE"
                     /locus_tag="ACHOT6_000597"
     CDS             48490..49080
                     /gene="caiE"
                     /locus_tag="ACHOT6_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFP9945873.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARSVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(49166..49561)
                     /gene="caiF"
                     /locus_tag="ACHOT6_000598"
     CDS             complement(49166..49561)
                     /gene="caiF"
                     /locus_tag="ACHOT6_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFP9945874.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(49822..53043)
                     /gene="carB"
                     /locus_tag="ACHOT6_000599"
     CDS             complement(49822..53043)
                     /gene="carB"
                     /locus_tag="ACHOT6_000599"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFP9945875.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFSGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVSLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(53061..54209)
                     /gene="carA"
                     /locus_tag="ACHOT6_000600"
     CDS             complement(53061..54209)
                     /gene="carA"
                     /locus_tag="ACHOT6_000600"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFP9945876.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPIFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(54665..55486)
                     /gene="dapB"
                     /locus_tag="ACHOT6_000601"
     CDS             complement(54665..55486)
                     /gene="dapB"
                     /locus_tag="ACHOT6_000601"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFP9945877.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(55653..56567)
                     /gene="rihC"
                     /locus_tag="ACHOT6_000602"
     CDS             complement(55653..56567)
                     /gene="rihC"
                     /locus_tag="ACHOT6_000602"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFP9945878.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(56633..57583)
                     /gene="ispH"
                     /locus_tag="ACHOT6_000603"
                     /gene_synonym="lytB"
     CDS             complement(56633..57583)
                     /gene="ispH"
                     /locus_tag="ACHOT6_000603"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFP9945879.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(57585..58034)
                     /gene="fkpB"
                     /locus_tag="ACHOT6_000604"
     CDS             complement(57585..58034)
                     /gene="fkpB"
                     /locus_tag="ACHOT6_000604"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9945880.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(58159..58653)
                     /gene="lspA"
                     /locus_tag="ACHOT6_000605"
     CDS             complement(58159..58653)
                     /gene="lspA"
                     /locus_tag="ACHOT6_000605"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFP9945881.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(58653..61469)
                     /gene="ileS"
                     /locus_tag="ACHOT6_000606"
     CDS             complement(58653..61469)
                     /gene="ileS"
                     /locus_tag="ACHOT6_000606"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFP9945882.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIELMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(61512..62453)
                     /gene="ribF"
                     /locus_tag="ACHOT6_000607"
     CDS             complement(61512..62453)
                     /gene="ribF"
                     /locus_tag="ACHOT6_000607"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFP9945883.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(62461..62679)
                     /gene="yaaY"
                     /locus_tag="ACHOT6_000608"
     CDS             complement(62461..62679)
                     /gene="yaaY"
                     /locus_tag="ACHOT6_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFP9945884.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            62782..63045
                     /gene="rpsT"
                     /locus_tag="ACHOT6_000609"
     CDS             62782..63045
                     /gene="rpsT"
                     /locus_tag="ACHOT6_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFP9945885.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        15300 a        17254 c        16463 g        14402 t
ORIGIN      
        1 aaatccgcaa aacatataat tcaatacaaa tcatcaggat aggttttgca acgcgtgcat
       61 tttgtcccct ttttcctcgt ggattagatg caaaaattta ttctgaaata tgtcaaccga
      121 tgaaaagcgt cggtagttaa gcagaaatta atatcgctta ctttaaccac cgcagcacaa
      181 ttagctaatt ttacggatgc agaactcatg ctggcgggac gtttttattg cgtcagggtt
      241 gacatccgtt tttgtatcca gtaactctaa aagcatatcg cattcatctg gagctgattt
      301 aatgactcac atcgttcgct ttatcggtct actactacta aacgcatctt ctttgcgcgg
      361 tagacgagtg agcggcatcc agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc
      421 attgcgcggg tttttttatg cccgaagcga ggcgctctaa aagagacaag gacccaaacc
      481 atgagccagc aagtcattat tttcgatacc acattgcgcg acggtgaaca ggcgttacag
      541 gcaagcttga gtgtgaaaga aaaactgcaa attgcgctgg cgcttgagcg tatgggtgtt
      601 gacgtgatgg aagtcgggtt ccccgtctct tcgccgggtg attttgaatc agtgcaaacc
      661 atcgctcgcc aggttaaaaa cagccgcgta tgcgcgttag ctcgctgcgt ggaaaaagat
      721 atcgacgtgg cggccgaatc cctgaaagtc gccgaagcct tccgtattca tacctttatt
      781 gccacttcgc caatgcacat cgccaccaag ctgcgcagca cgctggacga ggtgatcgaa
      841 cgcgctatct atatggtgaa acgcgcccgt aattacaccg atgatgttga attttcttgc
      901 gaagatgccg ggcgtacacc cattgccgat ctggcgcgag tggtcgaagc ggcaatcaat
      961 gccggtgcca ccaccatcaa cattccggac accgtgggct acaccatgcc gtttgagttc
     1021 gccggaatca tcagcggcct gtatgaacgc gtgcctaaca tcgacaaagc cattatttcc
     1081 gtacataccc acgacgattt gggcctggcg gtcggaaact cactggcggc ggtacatgcc
     1141 ggtgcacgcc aggtggaagg tgcaatgaac gggatcggcg agcgtgccgg taactgttcc
     1201 ctggaagaag tcatcatggc gatcaaagtt cgtaaggata ttctcaacgt ccacaccgcc
     1261 attaatcacc aggagatatg gcgcaccagc cagttagtta gccagatttg taatatgccg
     1321 atcccggcaa acaaagccat tgttggcagc ggcgcattcg cacactcctc cggtatccac
     1381 caggatggcg tgctgaaaaa ccgcgaaaac tacgaaatca tgacaccaga atctattggt
     1441 ctgaaccaaa tccagctgaa tctgacctct cgttcggggc gtgcggcggt gaaacatcgc
     1501 atggacgaga tggggtataa agaaagtgaa tataatttag acaatttgta cgacgctttc
     1561 ctgaagctgg cggacaaaaa aggtcaggtg tttgattacg atctggaggc gctggccttc
     1621 atcggtaagc agcaagaaga gccggagcat ttccgtctgg attacttcag cgtgcagtct
     1681 ggctctaacg atattgccac cgccgccgtc aaactggcct gtggcgaaga aatcaaagca
     1741 gaagccgcca acggtaacgg tccggtcgat gccgtctatc aggcgattaa ccgcatcact
     1801 gactataacg tcgaactggt gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg
     1861 ctgggtcagg tggatattgt cgccaactac aacggtcgcc gcttccacgg cgtcggcctg
     1921 gccaccgata ttgtcgagtc ctccgccaaa gccatggtgc acgtactgaa caatatctgg
     1981 cgcgccgcag aagtcgaaaa agagttgcaa cgcaaagctc aacacaacga aaacaacaag
     2041 gaaaccgtgt gatgtcgaag aattaccata ttgccgtatt gccgggggac ggtattggtc
     2101 cggaagtgat gacccaggcg ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc
     2161 gcatcaccac cagccattac gatgtaggcg gcgctgccat tgataaccac gggcagccgc
     2221 tgccgcctgc gacggttgaa ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag
     2281 gcggcccgaa gtgggaacat ttaccaccgg accagcaacc agaacgcggc gcactgttgc
     2341 ctttgcgtaa gcacttcaaa ttattcagca acctgcgtcc ggcaaaactg tatcaggggc
     2401 tggaagcatt ctgtccgctg cgtgctgaca ttgccgctaa cggcttcgac atcctgtgcg
     2461 tgcgcgaact gaccggcggc atctatttcg gtcagccaaa aggccgcgaa ggtagcggac
     2521 aatatgaaaa agcgtttgat accgaggtgt atcaccgttt tgagatcgag cgtattgccc
     2581 gcatcgcgtt tgaatctgcc cgcaagcgtc gccacaaagt cacctcaatc gacaaagcca
     2641 acgtgctgca atcctctatt ttatggcggg agatcgttaa cgagatcgcc acggaatacc
     2701 cggatgtcga actggcgcat atgtacatcg acaacgccac catgcagctg attaaagatc
     2761 catcacagtt tgacgttctg ctgtgctcca acctgtttgg cgacattctg tctgacgagt
     2821 gcgcaatgat cactggctcg atgggcatgt tgccttccgc cagcctgaac gaacaaggtt
     2881 ttggtctgta tgaaccggcg ggcggctcgg caccagatat cgcaggcaaa aacatcgcca
     2941 acccgattgc gcaaattctg tcgctggcat tgctgctgcg ctacagcctg gatgccgatg
     3001 atgcggcttc cgccattgaa cgcgccatta accgcgcatt agaagaaggc attcgcaccg
     3061 gggatttagc ccgtggcgct gccgccgtta gtaccgatga aatgggcgat atcattgccc
     3121 gctatgtggc agaaggggtg taatcatggc taagacgtta tacgaaaaat tgttcgacgc
     3181 gcacgtagtg tatgaagccg aaaacgaaac cccgctgtta tatatcgacc gccacctggt
     3241 gcatgaagtg acctcaccgc aggcgttcga tggtctgcgc gcccacggtc gcccggtacg
     3301 tcagccgggc aaaaccttcg ccaccatgga tcacaacgtc tctacccaga cgaaagacat
     3361 taatgcctgt ggtgaaatgg cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga
     3421 atttgacgtc gaactgtatg acctgaatca cccgtatcag gggatcgtcc acgtaatggg
     3481 gccggaacag ggcgtgacct tgccggggat gaccattgtc tgcggcgact cgcataccgc
     3541 cactcacggc gcgtttggcg cactggcctt tggtatcggc acttccgaag ttgaacacgt
     3601 actggcaacg caaaccctga aacagggccg tgcgaagacc atgaaaattg aagtccaggg
     3661 caaagccgcg ccgggcatta ccgcaaaaga tatcgtgctg gcaattatcg gtaaaaccgg
     3721 tagcgcaggc ggcaccgggc atgtggtgga gttttgcggc gaagcaatcc gtgatttaag
     3781 catggaaggt cggatgaccc tgtgcaatat ggcaatcgaa atgggcgcga aagccggtct
     3841 ggttgcgccg gacgaaacca cctttaacta tgtcaaaggc cgtctgcatg cgccgaaagg
     3901 caaagatttc gacgacgccg ttgcctactg gaaaaccctg caaaccgacg aagacgcaac
     3961 gttcgatacc gttgtcactc tgcaagcaga agaaatttca ccgcaggtca cctggggcac
     4021 caatccaggc caggtgattt ccgtgaacga caatattccc gatccggctt cgtttgccga
     4081 tccggttgaa cgcgcgtcgg cagaaaaagc gctggcctat atggggctga aaccgggtat
     4141 tccgctgacc gaagtggcta tcgacaaagt gtttatcggt tcctgtacca actcgcgcat
     4201 tgaagattta cgcgcggcag cggagatcgc caaagggcga aaagtcgcgc caggcgtgca
     4261 ggcactggtg gttcccggct ctggcccggt aaaagcccag gcggaagcgg aaggtctgga
     4321 caaaatcttt attgaagccg gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc
     4381 gatgaacaac gaccggctga atccgggcga acgttgtgcc tccaccagca accgtaactt
     4441 tgaaggccgc caggggcgcg gcgggcgcac gcatctggtc agcccggcaa tggctgccgc
     4501 cgcggcggtg accggacatt tcgccgacat tcgcaacatt aaataaggag cacaccatgg
     4561 cagagaaatt tatcaaacac acaggcctgg tggttccgct ggatgccgcc aatgtcgata
     4621 ccgatgcaat catcccgaaa cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc
     4681 atctgtttaa cgactggcgt tttctcgatg aaaaaggcca acagccaaac ccggacttcg
     4741 tgctgaactt cccgcagtat cagggcgctt ccattttgct ggcacgagaa aacttcggct
     4801 gtggctcctc gcgtgagcac gcgccctggg cattgaccga ctacggtttt aaagtggtga
     4861 ttgcgccgag ttttgctgac atcttctacg gcaatagctt taacaaccag ctgctgccgg
     4921 tgaaattaag cgatgcagaa gtggacgaac tgtttgcgct ggtgaaagcc aatccgggga
     4981 tccatttcga cgtggatctg gaagcgcaag aggtgaaagc gggagagaaa acctatcgct
     5041 ttaccatcga tgccttccgc cgccactgca tgatgaacgg tctggacagt attgggctta
     5101 ccttgcagca cgacgacgcc attgccgctt atgaagcaaa acaacctgcg tttatgaatt
     5161 aatccccttg cccggtcaaa tgaccgggct ttccgctatc gtccacgtca tcaaacgtct
     5221 ttaacctttg cggttaaaaa taatgcgaca acagaaataa ccgcaattac ccagtagaca
     5281 gcaaagtgcc cccaactttg tgcaattgct ccctgaataa cgccagccag aattaccccg
     5341 gtagaaatac tgttagtaaa taaggtggta gctgcccccg ctcttccagg cattaaatcc
     5401 tgaaaccata gcatcccaat acccgcaaca atgccgataa atacagcgtt aaaaagttgc
     5461 agcgtcatca acgccatacg gctatgaaag aaaatcaatc cggtgtaaaa cagtactcct
     5521 gccgccactg ctatgaccat cattcgccgc ttaccataac gtttgacata gtagccagcc
     5581 agaatcattg ctggtatttc cagtccagct gccgtcccca tcaggaaacc cgccagtttg
     5641 tctggcaatc ctaactcgct actgatccac aacggcatat caataatgta catggtgttg
     5701 caggtccaca ttaacgtcga ggcgacaaat aacatccgta cgttactatc ctgccagcca
     5761 ccttgcattg ataaagcatt ttccgacggc agttctaccc gcgccacaga cggaagcata
     5821 aatgcaatca ataccagact gagtgtgaat atcccggcgg caatcgaaaa catcaccgta
     5881 aagccgtaat tcaacgccag cataaaggcc aacggtggac cgataaccca tgccagagaa
     5941 agctgcgcac gcatcaccga gctaaacatc accacttctc gcgccgagtt atccgcatat
     6001 tcccgcgcca gagcaaataa ctgtggcatt gccgtattgg ccagagatgc cagaagcaca
     6061 ccacaggtga taagcgtcag ataatgacga ttaaatgcaa acaatagcgc attgccgata
     6121 gccatcaaac agcaaaatat aatcagtttt cgccgatcgc cctgactgtc agaacgtttt
     6181 gccaaccaga ggcttacgcc gatcccagca atagcattca ccgtataaaa gaggccgatc
     6241 cagaaaggtt gcgcgccaac ctcacgactc agaaataagc tcaatgtagg agcctgtagc
     6301 gccccggcca cccccatcat aaaagcgacc agcataaatg ccgcgtaaac accgttcata
     6361 cggcgagcca tcgtcattat ccagatcata cgttcccttt ttagcgcggc gagaataaaa
     6421 aaaaccagca ggtataatct gctggcgggt gattttacac caatactcag tcacacatga
     6481 tgcaggcaag tcaactttca gaattgcggt catcccactg catcagtcct tcaagcatgg
     6541 ttaatcgttg tgggacgctt aaccaacgca accagcacaa cttcgctgtc gcggtaaaat
     6601 agtgctgata aaactgacgc atggggcacc tccttgcttc atcgttagga attattgact
     6661 taatataggg aaaataaaat tgctgtcttt tgcgcaggag ttcccctttt atgccatctg
     6721 ctcgtctgca acaacagttc atccgcctgt ggcaatgctg cgagggtaaa tcgcaggaca
     6781 caacgctcaa cgaactggca gcgttattga gctgctcgcg tcgtcatatg cgcaccctgc
     6841 tcaacaccat gcaggatcgc ggctggctga cgtgggaagc ggaagtcggg cgtggtaaac
     6901 gctcgcgtct gacattcctc tataccgggc tggcgcttca gcaacagcgg gcggaagacc
     6961 tgctggagca ggatcgtatc gaccaactag tgcagttagt tggcgacaaa gcgactgtgc
     7021 ggcaaatgct ggtttctcat ctgggccgca gcttccgcca ggggcggcac atcctgcgcg
     7081 tgctctacta tcgtccgttg cgtaatctgc tacctggcag cgcattgcgc cgttccgaaa
     7141 cccatattgc ccggcaaatc ttcagttcgc taacgcgcat aaatgaggaa aatggggaac
     7201 tggaagcaga catcgcccac cactggcagc aaatatcacc gcttcactgg cgtttctttt
     7261 tgcgtccagg agtccatttt catcatggtc gtgaactgga aatggacgac gtgatcgcct
     7321 ctttaaaacg aatcaatacg ctgccgctct attcgcatat tgcggacatt gtgtcgccga
     7381 cgccctggac gctggatatc catctcacgc aaccggaccg ctggttaccg ttactgctgg
     7441 ggcaagttcc ggcgatgatc ctgccgcgcg aatgggaaac cctcagtaac tttgccagcc
     7501 atcccatcgg caccggtccg tatgcggtga tccgcaacac caccaatcaa ctgaaaattc
     7561 aggcattcga tgacttcttc ggttaccggg cattaatcga tgaagttaac gtctgggttc
     7621 tgccggaaat tgccgacgag ccagctggag gtctgatgct aaaaggtcca cagggcgagg
     7681 aaaaagagat tgaaagccgc ctggaggaag gttgctacta tttactattc gacagccgca
     7741 cccatcgcgg ggcgaatcag caagtcaggg actgggtaag ctatgtgctt tctccaacta
     7801 atctggtcta tttcgctgag gaacagtacc agcaactgtg gttcccggct tatggactgc
     7861 tcccccgttg gcaccatgcc cgcaccataa agagcgaaaa accggctggt atggaaagcc
     7921 tcaccctgac cttttatcag gatcacagtg agcatcgggt gattgccggg atcatgcagc
     7981 agattctggc aagccaccag gtcacgctgg aaatcaaaga gatcgactac gatcagtggc
     8041 atgaaggaga gatcgagagc gatatctggc ttaatagcgc caactttacg ctgccgctgg
     8101 atttttcgct gttcgcgcac ttgtgcgagg tgccgctgct ccaacactgt attccgatcg
     8161 actggcaagc cgacgccgcc cgctggcgca atggcgaaat gaacctggcg aactggtgcc
     8221 agcaactggt cgccagtaaa gcaatggtgc cgcttatcca ccactggctg atcattcagg
     8281 gacaacgcag tatgcgcggc ctgcgtatga ataccctcgg ctggttcgat tttaaatcag
     8341 cgtggtttgc gccgccggat ccatgattgc tggtagataa caaaatcact acactaacgc
     8401 cgttctcaac ggggtgccac gcaaacgcgt gcgctgagaa aatacccgtc gaacctgatc
     8461 cggataacgc cggcgaaggg atttgaggct ccttctcaag tcctttgcca ctcttttttg
     8521 aggtgcaaag tgttaaaaaa atgtcttccc ctgctgttgc tgtgcacagc gcccgttttc
     8581 gctaaacccg ttctgactgt ttatacctac gattccttcg ccgccgactg ggggcctggt
     8641 ccaaagatca aaaaagcttt tgaagccgac tgtaactgcg aactgaaact ggtggcgctg
     8701 gaagatggcg tttcgcttct caaccgtcta cggatggaag gcaaaaatag taaagccgat
     8761 gtggtgctgg ggctggataa caatctgtta gacgccgcca gtaaaaccgg actatttgcc
     8821 aaaagcggtg tggcagcgga tgccgttaac gttcccggcg gctggaataa tgacactttc
     8881 gtcccgtttg attacggcta tttcgccttc gtctatgaca agaacaaact gaaaaacccg
     8941 ccgcaaagcc tgaaagaatt ggttgagagc gatcagaact ggcgggtgat ttatcaggat
     9001 ccgcgcacca gtacgccggg gctgggcctg ctgttgtgga tgcaaaaagt ctatggcgat
     9061 gacgccccac aagcctggca aaaactggcg aagaaaaccg tcacggtcac caaaggctgg
     9121 agcgaagcct acggcctgtt tttaaaaggt gaaagcgatc tggtactgag ttacaccacc
     9181 tctccggctt atcacattct cgaagagaaa aaagataact acgccgccgc gaactttagc
     9241 gaaggtcact atctgcaggt ggaagtcgcc gcccgcaccg ctgccagcaa gcagccggag
     9301 ctggcgcaaa aattcctcca gtttatggtt tctccggctt ttcagaatgc gatcccaacc
     9361 ggtaactgga tgtatccggt ggcaaatgtc acgctgcctg ccgggtttga acaattgacc
     9421 aaaccggcca ccacgctgga gttcacacca gctgaagtgg cggcacaacg tcaggcatgg
     9481 attagcgaat ggcaacgcgc cgtcagccgt taattcccgg ctggttaatt ccaggtgtaa
     9541 gcgccgccac gctggtggta gcggttgcgc tcgcggcgtt tctcgccctg tggtggaacg
     9601 cgccgcaggg taactgggtg gcagtctggc aggacagcta cctgtggcat gtggtgcgct
     9661 tctccttctg gcaggcgttt ctctcggcgc tactctctgt cgtacccgcg atattcctcg
     9721 cccgcgccct ctatcgcagg cgctttccgg gtcggctggc gctgttgcgt ctgtgcgcaa
     9781 tgaccttgat cctcccggtg ctagttgctg ttttcggcat tcttagcgtc tatggtcgtc
     9841 agggctggct ggcatcgctc tgccaatcgc tcggtctgga gtggaccttt tcgccctacg
     9901 gcctgcaagg tattttgctg gcgcacgtat tttttaatct gccgatggcg agccgcttat
     9961 tactccaggc actggaaaac attcccggcg aacaacgtca gcttgccgcc cagcttggga
    10021 tgcgcggctg gcattttttc cgcttcgtcg aatggccgtg gttacggcga caaatcccgc
    10081 cggttgctgc acttatcttt atgctctgtt tcgccagctt cgccaccgtg ctatcactgg
    10141 gcggcggtcc gcaggcgacc actatcgagc tggctattta tcaggcgctg agttacgact
    10201 acgatcctgc ccgcgcggcg atgctggcgc tgatccagat ggtgtgttgc cttgggctgg
    10261 tactgttgag tcagcgattg agtaaggcca ttgcgccagg caccacgctg ctgcaaggct
    10321 ggcgcgaccc ggacgatcgt ctgcatagcc gcatttgcga cacggtgtta attgtgctgg
    10381 cgctgctgct gttgctgcca ccgttactgg cggtgatcgt cgatggcgta aatcgccagt
    10441 tgccggaagt gctggcacaa ccggtgctgt ggcaggcgct gtggacctcg ttgcgtattg
    10501 cgctggcggc aggtgtattg tgcgtagtgc tgaccatgat gctgctatgg agcagtcgcg
    10561 aactgcgggc gcggcagaaa atgctggcgg gtcaggcgct ggagatgagc ggcatgttga
    10621 tcctcgccat gccggggatt gtgctggcta ccggcttctt tttactgctc aacaacacca
    10681 ttggcctgcc gcaatctgct gacggcattg tgattttcac caatgcgtta atggcgatcc
    10741 cttatgcgct gaaagtactg gaaaacccga tgcgcgatat caccgcccgc tacagtatgt
    10801 tgtgtcagtc gctgggcatt gaaggctggt cgcgcttaaa agtggtcgag ctgcgcgccc
    10861 tgaaacgtcc actggcgcag gcgctggctt ttgcctgcgt gctgtcgatt ggtgattttg
    10921 gcgtagtggc gttgttcggt aacgatgatt tccgtaccct gccgttttat ctctaccagc
    10981 aaattggctc ctatcgcagc caggacggtg cggtcaccgc gttaattctg ctactactct
    11041 gttttctgct gtttaccgtg attgaaaaac taccggggcg aaatgttaaa actgactgat
    11101 atcacctggc tttatcacca tttgccgatg cgttttagct taacggtaga acgcggcgag
    11161 caggtggcaa tcctcgggcc aagcggcgcg ggtaaaagta ccctgctgaa tttgatcgcc
    11221 ggttttctga cgccagccag cggttcgctg actatcgacg gcgttgatca caccacgacg
    11281 ccgccgtcac gccgtccggt gtcgatgctg tttcaggaaa acaacctgtt cagtcacctg
    11341 acggtcgcgc agaacatcgg gctggggcta aatccgggat tgaaactgaa cgcggcacag
    11401 caggagaaaa tgcacgctat cgcccgccag atggggattg ataatttaat ggcgcggtta
    11461 cctggcgagc tttccggcgg tcagcgacag cgcgtggcgt tagcgcgttg tctggtgcgc
    11521 gaacagccga ttttattgct cgatgaaccg ttctctgcgc tcgatccggc gctacgtcag
    11581 gagatgctga cgctggtgag cacgagctgc cagcagcaaa aaatgacgct attgatggtg
    11641 tcgcacagtg tggaagatgc ggcgcggatc gccacacgct cggtggtggt cgccgacggg
    11701 cgcatcgcct ggcagggtaa gactgaagaa ttgttgagcg gtaaggcgag tgcttcggca
    11761 atattgggga ttacgggtta gtatgcactg tcggatgcgg cgtgaacgcc ttatccgacc
    11821 tacgggtagt gcgttttgta ggcctgataa gacgcggtca gcgtcgcatc aggcaacccg
    11881 cacaagacta tctcctaacc cccaaccact ttacgcaaaa tatcgatata caccggcatc
    11941 aacgggtggc gaattaacac caccagcgcc accacgccga tggcagaaat taacggcgtc
    12001 agccacaaca aacggccgcg cgacaaataa tgacttaaac gatcggttgc tttaccgctg
    12061 cgccataacc gccagcacag ccagccacca acccacaaaa ataccgccgt cgccagcagc
    12121 aaccatttaa actcgccgct ctgcattccg gctggaatat cgatcgccgc gcccgccaga
    12181 atcccaggca ggaagtaaaa cggtggccac agcaggcagc cgataatatt cggcgtaatg
    12241 aattttgcca ccggcagatc cagcattccc gccaccattg gcaccagcgg acgcgtcggg
    12301 ccaacaaagc gaccgaccag aatggtgaac atgctgtgtt gatgcaacgc atgttcagtt
    12361 ttatcgagta atgctttgtt tttcttcaga aatgaccagc gatgcaacgg ctttttaaaa
    12421 cgccagccca gccagaagga gatccagtcg cccagcagac aaccgacaat ccctgccagc
    12481 caggcgtgcc agaaacttaa ctcgccgctg ccaatcagcg ctcccagccc cgccatcagc
    12541 accgtaccgg gtagaatcaa accgaccagc gccagcgact ccagaaaggc caccaacacc
    12601 accgccatca atgaatacac ggtagattgg gtaataaagt gttccagcaa tgcttgcata
    12661 atgtgcctgt caaatggacg aagcagggat tctgcaaacc ctatgctact ccgtcaagcc
    12721 gtcaattgtc tgattcgtta ccaattatga caacttgacg gctacatcat tcactttttc
    12781 ttcacaaccg gcacggaact cgctcgggct ggccccggtg cattttttaa atactcgcga
    12841 gaaatagagt tgatcgtcaa aaccaacatt gcgaccgacg gtggcgatag gcatccgggt
    12901 ggtgctcaaa agcagcttcg cctggctgat acgttggtcc tcgcgccagc ttaagacgct
    12961 aatccctaac tgctggcgga aaagatgtga cagacgcgac ggtgacaagc aaacatgctg
    13021 tgcgacgctg gcgatatcaa aattgctgtc tgccaggtga tcgctgatgt actgacaagc
    13081 ctcgcgtacc cgattatcca tcggtggatg gagcgactcg ttaatcgctt ccatgcgccg
    13141 cagtaacaat tgctcaagca gatttatcgc cagcagctcc gaatagcgcc cttccccttg
    13201 cccggcgtta atgatttgcc caaacaggtc gctgaaatgc ggctggtgcg cttcatccgg
    13261 gcgaaagaaa cccgtattgg caaatattga cggccagtta agccattcat gccagtaggc
    13321 gcgcggacga aagtaaaccc actggtgata ccattcgcga gcctccggat gacgaccgta
    13381 gtgatgaatc tctcctggcg ggaacagcaa aatatcaccc ggtcggcaaa caaattctcg
    13441 tccctgattt ttcaccaccc cctgaccgcg aatggtgaga ttgagaatat aacctttcat
    13501 tcccagcggt cggtcgataa aaaaatcgag ataaccgttg gcctcaatcg gcgttaaacc
    13561 cgccaccaga tgggcattaa acgagtatcc cggcagcagg ggatcatttt gcgcttcagc
    13621 catacttttc atactcccgc cattcagaga agaaaccaat tgtccatatt gcatcagaca
    13681 ttgccgtcac tgcgtctttt actggctctt ctcgctaacc caaccggtaa ccccgcttat
    13741 taaaagcatt ctgtaacaaa gcgggaccaa agccatgaca aaaacgcgta acaaaagtgt
    13801 ctataatcac ggcagaaaag tccacattga ttatttgcac ggcgtcacac tttgctatgc
    13861 catagcattt ttatccataa gattagcgga tcctacctga cgctttttat cgcaactctc
    13921 tactgtttct ccatacccgt ttttttggat ggagtgaaac gatggcgatt gcaattggcc
    13981 tcgattttgg cagtgattct gtgcgagctt tggcggtgga ctgcgccagc ggtgaagaga
    14041 tcgccaccag cgtagagtgg tatccccgtt ggcaaaaagg gcaattttgt gatgccccga
    14101 ataaccagtt ccgtcatcat ccgcgtgact acattgagtc aatggaagcg gcactgaaaa
    14161 ccgtgcttgc agagcttagc gtcgaacagc gcgcagctgt ggtcgggatt ggcgttgaca
    14221 caaccggctc gacgcccgca ccgattgatg ccgacggtaa cgtgctggcg ctgcgcccgg
    14281 agtttgccga aaacccgaac gcgatgttcg tattgtggaa agaccacact gcggttgaag
    14341 aagcggaaga gattacccgt ttgtgccacg cgccgggcaa cgttgactac tcccgctaca
    14401 ttggcggtat ttattccagc gaatggttct gggcaaaaat cctgcatgtg actcgccagg
    14461 acaccgccgt ggcgcaatct gccgcatcgt ggattgagct gtgcgactgg gtgccagctc
    14521 tgctttccgg taccacccgc ccgcaggata ttcgtcgcgg acgttgcagc gccgggcata
    14581 agtcgctatg gcacgaaagc tggggtggct tgccgccagc cagtttcttt gatgagctgg
    14641 acccgatcct caatcgccat ttgccttccc cgctgttcac tgacacctgg actgccgata
    14701 ttccggtggg caccttatgc ccggaatggg cgcagcgtct cggcctgcct gaaagcgtgg
    14761 tgatttccgg cggcgcgttt gactgccata tgggcgcagt tggcgcaggc gcacagccta
    14821 acgcactggt aaaagttatc ggtacttcca cctgcgacat tctgattgcc gacaaacaga
    14881 gcgttggcga gcgggcagtg aaaggtattt gcggtcaggt tgatggcagc gtggtacctg
    14941 gatttatcgg tctggaagca ggccaatcgg cgtttggtga tatctacgcc tggtttggtc
    15001 gcgtactcgg ctggccgctg gaacagcttg ccgcccagca tccggaactg aaagcgcaaa
    15061 tcaacgccag ccagaaacaa ctgcttccgg cgctgaccga agcatgggcc aaaaatccgt
    15121 ctctggatca cctgccggtg gtgctcgact ggtttaacgg ccgccgcaca ccgaacgcta
    15181 accaacgcct gaaaggggtg attaccgatc ttaacctcgc taccgacgct ccgctgctgt
    15241 tcggcggttt gattgctgcc accgcctttg gcgcacgcgc aatcatggag tgctttaccg
    15301 atcaggggat cgccgtcaat aacgtgatgg cgctgggcgg catcgcgcgg aaaaaccagg
    15361 tcattatgca ggcctgctgc gacgtgctga atcgcccgct gcaaattgtt gcctctgacc
    15421 agtgctgtgc gctcggtgcg gcgatttttg ctgccgtcgc cgcgaaagtg cacgcagaca
    15481 tcccatcagc ccagcaaaaa atggccagtg cggtagagaa aaccctgcaa ccgcgcagcg
    15541 aacaggcaca acgctttgaa cagctttatc gccgctatca gcaatgggcg atgagcgccg
    15601 aacaacacta tcttccaact tccgccccgg cacaggctgc ccaggccgtt gcgactctat
    15661 aaggacacga taatgacgat ttttgataat tatgaagtgt ggtttgtcat tggcagccag
    15721 catctgtatg gcccggaaac cctgcgtcag gtcacccaac atgccgagca cgttgttaat
    15781 gcgctgaata cggaagcgaa actgccctgc aaactggtgt tgaaaccgct gggcaccacg
    15841 ccggatgaaa tcaccgctat ttgccgcgac gcgaattacg acgatcgttg cgctggtctg
    15901 gtggtgtggc tgcacacctt ctccccggcc aaaatgtgga tcaacggcct taccatgctc
    15961 aacaaaccgt tgctgcaatt ccacacccag tttaacgcgg cgctgccgtg ggacagtatc
    16021 gatatggact ttatgaacct gaaccagact gcacatggcg gtcgcgagtt cggcttcatt
    16081 ggcgcgcgta tgcgtcagca acatgccgtg gttaccggtc actggcagga taaacaagcc
    16141 catgagcgta tcggctcctg gatgcgccag gcggtctcta aacaggatac ccgtcatctg
    16201 aaagtctgcc gttttggcga taacatgcgt gaagtagcgg tcaccgatgg tgataaagtt
    16261 gccgcacaga tcaagttcgg tttctccgtc aatacctggg cggttggcga tctggtgcag
    16321 gtggtgaact ccatcagcga cggcgatgtt aacgcgctgg tcgatgagta cgaaagctgc
    16381 tacaccatga cgcctgccac acaaatccac ggcgaaaaac gacagaacgt gctggaagcg
    16441 gcgcgtattg agctggggat gaagcgtttc ctggaacaag gtggcttcca cgcgttcacc
    16501 accacctttg aagatttgca cggtctgaaa cagcttccag gtctggccgt acagcgtctg
    16561 atgcagcagg gttacggctt tgcgggcgaa ggcgactgga aaaccgccgc cctgcttcgc
    16621 atcatgaagg tgatgtcaac cggtctgcag ggcggcacct cctttatgga ggactacacc
    16681 tatcacttcg agaaaggtaa tgacctggtg ctcggctccc atatgctgga agtctgcccg
    16741 tcgattgccg tagaagagaa accgatcctc gacgttcagc atctcggtat tggtggtaag
    16801 gacgatcctg cccgactgat cttcaatacc caaaccggtc cagcgattgt cgccagcctg
    16861 attgatctcg gcgatcgtta ccgtctgctg gttaactgta tcgacacggt gaaaacaccg
    16921 cactccctgc cgaaactgcc ggtggcgaat gcgctgtgga aagcgcaacc ggatctgcca
    16981 actgcttccg aagcgtggat cctcgctggt ggcgcgcacc ataccgtctt cagccatgcg
    17041 ctgaacctca acgatatgcg ccagttcgcc gagatgcacg acattgaaat cacggtgatt
    17101 gataacgaca cccgcctgcc agcgtttaaa gacgcactgc gctggaacga agtgtattac
    17161 ggatttcgtc gctaagtagc cacatcaggt atgtaacgcc tgatgcgacg ctgacgcgtc
    17221 ttatctggcc tacacgctgc gattttgtag gccggataag caaagcgcat ccggcattgt
    17281 tttccccctc gcccctttgg ggagagggcc ggggtgaggg gaaacgacct ctcaggagtc
    17341 aacatgttag aagatctcaa acgccaggta ttagaagcca acctggcgct gccaaaacac
    17401 aacctggtca cgctcacatg gggcaacgtc agcgccgttg atcgcgagcg cggcgtcttt
    17461 gtgatcaaac cttccggcgt cgattacagc gtcatgaccg ctgacgatat ggtcgtggtt
    17521 agcatcgaaa ccggtgaagt ggttgaaggt acgaaaaagc cctcctccga cacgccaact
    17581 caccggctgc tctatcaggc attcccctcc attggcggca ttgtgcatac gcactcgcgc
    17641 cacgccacca tctgggcgca gtcgggtcag tcgattccag caatcggcac cacccacgcc
    17701 gactatttct acggcaccat tccctgcacc cgcaaaatga ccgacgcaga aatcaacggc
    17761 gaatatgagt gggaaaccgg taacgtcatc gtagaaacct ttgaaaaaca gggtatcgat
    17821 gcagcgcaaa tgcccggcgt tctggtccat tcccacggcc cgtttgcatg gggcaaaaat
    17881 gccgaagatg cggtgcataa cgccatcgtg ctggaagagg tcgcttatat ggggatattc
    17941 tgccgtcagt tagcgccgca gttaccggat atgcagcaaa cgctgctgga taaacactat
    18001 ctgcgtaagc atggcgcgaa ggcatattac gggcagtaat gactgtataa aaccacagcc
    18061 aatcaaacga aaccaggcta tactcaagcc tggttttttg atggattttc agcgtggcgc
    18121 aggcaggttt tatcttaacc cgacactggc gggacacccc gcaagggaca gaagtctcct
    18181 tctggctggc gacggacaac gggccgttgc aggttacgct tgcaccgcaa gagtccgtgg
    18241 cgtttattcc cgccgatcag gttccccgcg ctcagcatat tttgcagggt gaacaaggct
    18301 ttcgcctgac accgctggcg ttaaaggatt ttcaccgcca gccggtgtat ggcctttact
    18361 gtcgcgccca tcgccaattg atgaattacg aaaagcgcct gcgtgaaggt ggcgttaccg
    18421 tctacgaggc cgatgtgcgc ccgccagaac gctatctgat ggagcggttt atcacctcac
    18481 cggtgtgggt cgagggtgat atgcacaatg gcactatcgt taatgcccgt ctgaaaccgc
    18541 atcccgacta tcgtccgccg ctcaagtggg tttctataga tattgaaacc acccgccacg
    18601 gtgagctgta ctgcatcggc ctggaaggct gcgggcagcg catcgtttat atgctggggc
    18661 cggagaatgg cgacgcctcc tcgcttgatt tcgaactgga atacgtcgcc agccgcccgc
    18721 tgctgctgga aaaactcaac gcctggtttg caaaccacga tcccgatgtg atcatcggct
    18781 ggaacgtggt gcagttcgat ctgcgaatgc tgcaaaaaca tgccgagcgt taccgtcttc
    18841 cgctgcgtct tgggcgcgat aatagcgagc tggagtggcg cgagcacggc tttaaaaacg
    18901 gcgtcttttt tgcccaggct aaaggtcggc taattatcga cggtatcgag gcgctgaaat
    18961 ccgcgttctg gaatttctct tcattctcgc tggaaactgt cgctcaggag ctattaggcg
    19021 aaggaaaatc tatcgataac ccgtgggatc gaatggacga aattgaccgc cgtttcgccg
    19081 aagataaacc tgcgctggca acttataacc tgaaagattg cgagctggtg acgcagatct
    19141 accacaaaac tgaaatcatg ccatttttac tcgaacgagc aacggtgaac ggcctgccgg
    19201 tggatcggca cggcggttcg gtggcagcgt ttggtcatct ctattttccg cgaatgcatc
    19261 gcgctggtta tgtcgcgcct aatctcggcg aagtgccacc gcacgccagc cctggcggct
    19321 acgtgatgga ttcacggcca gggctttatg attcagtgct ggtgctggac tataaaagcc
    19381 tgtacccgtc gatcatccgc acctttctga ttgatcccgt cgggctggtt gaaggcatgg
    19441 cgcaacctga tccagagcac agtaccgaag gttttctcga tgcctggttc tcacgggaga
    19501 aacattgcct gccggagatt gtgactaaca tctggcacgg gcgcgatgaa gccaaacgtc
    19561 agggtaataa gccgctttcg caggcgctga agatcattat gaatgccttt tatggcgtgc
    19621 tcggcaccac cgcctgccgc ttcttcgatc cgcgcctggc atcgtcgatc accatgcgtg
    19681 gtcatcagat catgcggcaa accaaagcat tgattgaagc gcagggctac gacgttatct
    19741 acggagatac cgactcgact tttgtctggc taaaaggtgc acattcggaa gaagaggcgg
    19801 cgaaaatcgg tcgtgcactg gtgcagcacg ttaacgcctg gtgggcggaa acgctgcaaa
    19861 aacaacggct gaccagcgca ttagaactgg agtatgaaac tcatttctgc cgttttctta
    19921 tgccaaccat tcgcggagct gataccggca gtaaaaagcg ctacgccggg ctgattcagg
    19981 agggcgacaa gcagcggatg gtgtttaaag ggctggaaac cgtgcgcacc gactggacgc
    20041 cgctggccca gcagtttcag caggagctgt acctgcgtat cttccgcaac gagccatatc
    20101 aggagtacgt ccgccaaacc atcgacaaac tgatggcggg tgaactggat gcgcggctgg
    20161 tttaccgtaa acgccttcgc cgtccgctga gcgaatatca gcgtaatgtt ccgcctcatg
    20221 tacgcgccgc tcgccttgcc gatgaagaaa atcaaaagcg cggtcgcccc ttgcaatatc
    20281 agaatcgcgg caccattaag tacgtatgga ccaccaacgg cccggagccg ctggactacc
    20341 aacgttcacc actggattac gaacactatc tgacccgcca gctacaaccc gtggcggagg
    20401 gaatactccc ttttattgag gataattttg ctacacttat gaccgggcaa cttgggctat
    20461 tttgagcaaa aaaaagagtt cgccagatac cattttgatg cgtgacgaat gctttgccat
    20521 ccagtaccat agcgcccttt ccattcctgg acctgaataa caccactacc tcataagcac
    20581 ggtagcgggt ggttattgcc tgcaattaaa gatatagagc cgaacacata tgccttttac
    20641 acttggtcaa cgctggatca gcgatacaga aagcgaattg ggacttggaa ccgttgtcgc
    20701 ggtggatgcg cgaactgtca ctttactttt cccatctact ggtgaaaacc gtctgtacgc
    20761 acgcagtgat tcccccgtga cccgcgtgat gttcaaccct ggtgatacca ttaccagcca
    20821 tgacggctgg cagatgcaag ttgaagaagt aaaagaagaa aatggcttgc tgacctatat
    20881 cggtactcgc ctggatactg aagagtccgg cgtagccctg cgtgaagttt tccttgatag
    20941 caaactggtg ttcagcaaac cgcaggaccg tctgtttgcc gggcagattg accgtatgga
    21001 ccgctttgcg ctgcgttatc gcgcgcgtaa gtattccagc gaacagttcc gtatgccgta
    21061 cagcggcctg cgcggtcagc gtaccagcct gattccgcat cagctcaaca tcgctcatga
    21121 tgttggtcgc cgccacgcgc cgcgcgtcct gctggctgac gaagtgggtt tagggaaaac
    21181 cattgaagcc gggatgatcc tgcatcagca actgctctct ggcgctgctg aacgtgtgct
    21241 aattatcgtc ccggaaacct tacagcatca gtggctggta gaaatgctgc gccgtttcaa
    21301 cctgcgcttt gcgctatttg atgatgagcg ttatgccgaa gctcagcacg atgcttacaa
    21361 cccgttcgac accgaacagc tggtgatttg ctcgctggat tttgcccgtc gtagcaaaca
    21421 gcgcctggaa catctctgtg aagccgaatg ggacctgctg gtggtcgatg aagcgcatca
    21481 cctggtgtgg agcgaagatg cgccaagccg tgaatatcag gccattgaac aactggcaga
    21541 gcacgtgccg ggcgttctgc tgctgaccgc gaccccggaa cagctgggga tggaaagcca
    21601 cttcgcccgt ctgcgtctgc tggacccgaa ccgtttccac gattttgcgc agttcgttga
    21661 agagcagaaa aattatcgtc cggttgcgga cgccgttgcc atgctgctgg caggtaacaa
    21721 actgagcaat gacgaactga acatgctcgg cgagatgatc ggcgagcagg atatcgagcc
    21781 gctgttgcag gcagcaaaca gcgacagcga agatgcccag agcgcccgtc aggagctggt
    21841 ttcgatgctg atggatcgcc acggcaccag ccgcgtgctg ttccgtaaca cccgtaacgg
    21901 tgtgaaaggc ttcccgaaac gcgagctgca caccattaag ctgccgctgc cgacgcagta
    21961 tcagacggct attaaagtct ccggcattat gggcgcacgc aaaagtgcgg aagatcgcgc
    22021 tcgcgatatg ctctacccgg agcgtattta tcaggaattt gaaggtgata acgccacctg
    22081 gtggaacttc gatccgcgcg ttgagtggct gatgggctac ctgaccagcc atcgctctca
    22141 gaaagtgctg gtgatctgcg ctaaagccgc taccgcgctg caactggagc aggtactgcg
    22201 cgaacgtgaa ggtatccgcg ccgcggtgtt ccacgaaggc atgtcgatta ttgagcgtga
    22261 ccgcgctgcc gcctggtttg ccgaagaaga caccggcgca caggtactgc tgtgctcgga
    22321 aatcggttct gaaggacgta acttccagtt cgccagccac atggtaatgt ttgacctgcc
    22381 attcaacccg gatctgctgg agcagcgtat tggtcgtctg gatcgtatcg gtcaggcgca
    22441 cgatattcag atccatgtgc cttatctgga aaaaactgct cagtcggtgc tggtgcgctg
    22501 gtatcacgaa ggtctggatg cattcgagca cacctgcccg accggacgca ctatttacga
    22561 tagcgtatac aacgatctga ttaactatct ggcttcaccg gatcaaaccg aaggctttga
    22621 cgatctgatc aaaaactgcc gcgagcagca tgaagcgctg aaagcacagc tggaacaggg
    22681 tcgtgaccgc ctgctggaaa tccactccaa cggtggcgaa aaagcccagg cgctggcaga
    22741 aagcattgaa gagcaggatg acgataccaa cctgatcgcc ttcgccatga acctgttcga
    22801 tattatcggt atcaatcagg acgatcgcgg cgacaacatg atcgtgctga cgccgtccga
    22861 tcatatgctg gtgccggact tccctggcct gtcggaagat ggcatcacca tcacctttga
    22921 tcgtgaagtg gcgctggcgc gtgaagatgc gcagtttatt acctgggagc atccgctgat
    22981 ccgcaacggt ctggatctga tcctttctgg cgatactggt agcagcacga tttcactgtt
    23041 aaaaaacaaa gcgttgccgg taggtacgct gttggtggaa ctgatttatg tcgttgaagc
    23101 ccaggctccg aagcagttgc agctcaaccg cttcctgcca ccgacgccgg tacgtatgct
    23161 gctggataaa aacggcaaca acctggcggc gcaggtagaa tttgaaacct ttaaccgtca
    23221 gcttaacgcg gttaaccgtc acaccggcag caaactggtt aacgccgtgc agcaggatgt
    23281 tcacgctatc cttcagctgg gtgaagcgca gatcgagaaa tctgcccgtg cattgattga
    23341 tgccgcacgt aacgaagccg acgaaaaact gtctgccgag ctgtctcgtc tggaagcgct
    23401 gcgcgcagtg aacccgaaca ttcgtgacga cgaactgacc gccattgaga gcaaccgtaa
    23461 gcaggtaatg gaaagcctgg atcaggcagg ctggcgtctg gatgccctgc gtttgatcgt
    23521 tgtgacgcat cagtaacgga gccgaaaatg gggatggaaa actacaatcc accgcaggaa
    23581 ccctggttgg ttatcctgta tcaggatgac cacattatgg tggtcaacaa gccgagcggt
    23641 ttgttgtcag tgccgggtcg tctggaagag cacaaagata gcgtgatgac gcgcattcag
    23701 cgtgattatc cgcaggcaga atcggtgcat cgtctggata tggctaccag cggcgtgatt
    23761 gtagtggcgc tgaccaaagc cgcggagcgg gagttaaaac gccagttccg cgagcgcgag
    23821 ccgaaaaagc aatatgtggc ccgcgtctgg gggcatccat cccctgcgga aggtctggtg
    23881 gatctgccgc tgatttgcga ctggccaaac cgcccgaaac agaaagtctg ttacgaaacg
    23941 ggcaagcctg cgcagacgga atatgaagtg gtggagtatg cggcggataa cacggcaaga
    24001 gtggtgttaa aaccgattac cgggcgttcg catcaattgc gtgtgcatat gctggcgctg
    24061 ggtcatccga ttctcggcga tcgtttttat gcatcaccag aagcgagagc gatggcaccg
    24121 cgtttgttgc tacatgcaga gatgttgacg attacccatc cggcgtatgg caatagtatg
    24181 acgtttaaag cgccagcgga tttttaatcg tcacgctggc gcagttcggt aggcggataa
    24241 ggcgttcacg ccgcatccgg caattgtgcg ccgattgcct gatgcgacgc gggccgcgtc
    24301 ttatcaggcc tacagtttaa aacttattta aaccctttct gctgctttat cagctcatat
    24361 gcctgctgaa tttcctgcgc tttctgcttc gccatctcca tcatctcagg cggcaaacct
    24421 ttcgccacca gcttatcggg atggtgttca ctcatcagct tacggtaggc acgtttgatg
    24481 gtggtcgcat catccgtcgg cttcacgccc agcacattac aggcatcttc cagcgttggg
    24541 ccacgctgcg cttgctgcca gttaccaccg ccagtttgct gctgataacc gccgccaaac
    24601 tgtgcaccgc cctgcatcat gcgcaaaaac tggtcaaact gagcgcggga gatccctaat
    24661 tcttctgcaa tgacatacag caccgcccgt tcattcgggt gcagtgaacc atcagcaaac
    24721 gccgcctgaa tctggatctc cagaaacata cgaattaagt caaaacgacc aaagcagaca
    24781 ctgcgaaact ggcgcatctt ttcgcgcagc gggtaattgt ctgattttcc cacccggaac
    24841 gcgttttgcg ccgcagtacg ggaagcgcca tgaaggttca ttcggtccat cagctggctg
    24901 gcgatatgaa tatcagcctc cgtgacgcga cctttggatt tggttaaatg ccccataact
    24961 tcaaaagtgg tggcaaaaaa cagcgcctga cgctcacgct ggttggcgaa ccacgccatt
    25021 ttacggctac gggctttatc aaacatatgg ccaattaaca ggcctaacac tacgccccaa
    25081 aagccgccgc ccatcagtaa ggccacggcc acgccaatga tttttcccca atactgcata
    25141 tattccccaa atcgacacac ggatatcagg gctatctccc acaatataaa ggtgctttta
    25201 ccgttttccg gctgcggtca attgtgggac atcgcctata atttgcatta tcatacctgt
    25261 cattcaatgc cgtgcctaac accactgacg ctattcggac aggattaaca ctagcgtaga
    25321 gatgacgagt acgttagtct ctgaccgttt gtcacgcgca acgttaccga tgatggaaca
    25381 ataaaatcaa cgtatgaaaa aacgtatccc cactctcctg gccaccatga ttgccaccgc
    25441 cctttatagt caacagggac tggcagccga tctcgcctca cagtgcatgt tgggcgtgcc
    25501 aagctatgac cgtcctctgg tacagggcga taccaatgac ttacccgtga ctatcaatgc
    25561 tgaccacgcg aaaggggact acccggatga cgccgtgttt actggcagcg tggatatcat
    25621 gcagggtaac agccgtctgc aggccgacga agtgcagctc catcaaaaag aggcaccagg
    25681 acaaccggag ccggtacgta ccgttgatgc gctcggtaat gtccattacg acgataacca
    25741 ggtgatcctc aaagggccga aaggctgggc gaatctgaac accaaagata ccaacgtctg
    25801 gaaaggtgat taccagatgg tgggtcgcca gggccgcggt aaagcggacc tgatgaaaca
    25861 acgtggtgaa aaccgctata ccattctgga taacggtagc tttacctcct gtctgccggg
    25921 ttctgacacc tggagcgtgg taggtagcga aattattcat gaccgcgaag aacaagttgc
    25981 ggagatctgg aacgcccgct ttaaggtggg tccggtaccg atcttttata gcccctattt
    26041 gcagttgccg gtgggtgaca aacgtcgctc tggtttcttg atcccgaacg ccaagtacac
    26101 caccaccaac tactttgagt tctacctgcc atattactgg aacatcgcgc caaatatgga
    26161 tgccaccatc acgccgcatt atatgcatcg tcgtggtaac atcatgtggg agaacgaatt
    26221 ccgctacctc tcccaggcgg gcgctggctt gatggaactg gactatctgc cttcagataa
    26281 agtctatgaa gatgaacacc cgaacgatga cagttcacgt cgttggttat tctactggaa
    26341 ccactccggg gtcatggatc aagtgtggcg tttcaacgtc gactacacca aggtcagcga
    26401 tcctagctac ttcaatgatt tcgataacaa gtacggttcc agtactgacg gctacgcaac
    26461 gcaaaaattc agcgttggct atgcggtgca aaacttcaat gccaccgttt caaccaagca
    26521 gttccaggtt ttcagcgaac agaacaccag tagctactcg gcagagccgc agttagacgt
    26581 taattactac cagaatgatg ttggtccgtt tgatacgcgt atttacggcc aggcagtgca
    26641 ctttgttaac accagagacg acatgcctga agcaacccgt gttcacctgg aaccgaccat
    26701 caatttgccg ctctctaata actggggcag catcaatacc gaagcgaagt tgctggcaac
    26761 ccattatcag caaaccaatc ttgactggta taactccaga aacacgacca agctggacga
    26821 atccgttaac cgcgtaatgc cgcaattcaa agttgacggc aaaatggtct ttgaacgcga
    26881 tatggaaatg ctggctccgg gttataccca aacgctggaa ccgcgcgcgc agtatttgta
    26941 cgtgccgtat cgcgatcaga gcgacatcta taactacgac tcgtctctgc tgcaatctga
    27001 ctactctggc ctgttccggg accggactta cggcggtctt gaccgtattg cctccgctaa
    27061 ccaggtgacg accggtgtca catctcgcat atatgatgat gctgccgttg aacgttttaa
    27121 tatttccgtt ggtcaaatct actatttcac ggagtctcgc actggcgatg acaacataac
    27181 atgggagaat gacgacaaaa cgggttcact ggtgtgggca ggcgatactt actggcgtat
    27241 ctccgagcgt tggggattgc gtggcgggat tcagtacgat acacgtctgg ataacgtagc
    27301 gaccagtaac tccagcattg aataccgtcg ggatgaagac cgtctggtac agctgaatta
    27361 ccgttacgcc agcccggaat atattcaggc tacgctgcct aagtactatt ccactgctga
    27421 gcaatataag aatggtattt cgcaggtagg tgctgtcgcc agctggccaa ttgccgatcg
    27481 ttggtccatt gttggggcct actactacga caccaatgcg aacaagcaag ccgactctat
    27541 gttaggtgtg caatacagct cctgctgcta tgcaattcgc gtcggttacg agcggaagct
    27601 gaacggttgg gataacgata aacaacatgc ggtatatgac aacgcaatcg gctttaacat
    27661 cgaacttcgc ggcctgagct ccaactacgg tctgggtacg caagagatgc tgcgttcgaa
    27721 cattctgccg tatcaaaaca ctttgtgatt tgttgattta ccacgtaatc cgcagtgcgg
    27781 ttaattgaaa tggaaaaagt atgaagaact ggaaaacgct gcttctcggt atcgccatga
    27841 tcgcgaatac cagtttcgct gccccccagg tagtcgataa agtcgcagcc gtcgtcaata
    27901 acggcgtcgt gctggaaagc gacgttgatg gattaatgca gtcggtaaaa ctgaacgctg
    27961 ctcaggcaag gcagcaactt cctgatgacg cgacgctgcg ccaccaaatc atggaacgtt
    28021 tgatcacgga tcaaatcatc ctgcagatgg ggcagaaaat gggagtgaaa atctccgatg
    28081 agcagctgga tcaggcgatt gctaacatcg cgaaacagaa caacatgacg ctggatcaga
    28141 tgcgcagccg tctggcttac gatggactga actacaacac ctatcgtaac cagatccgca
    28201 aagagatgat tatctctgaa gtgcgtaaca acgaggtgcg tcgtcgcatc accatcctgc
    28261 cgcaggaagt cgaatccctg gcgcagcagg tgggtaacca aaacgacgcc agcactgagc
    28321 tgaacctgag ccacatcctg atcccgctgc cggaaaaccc gacctctgat caggtgaacg
    28381 aagcggaaag ccaggcgcgc gccattgtcg atcaggcgcg taacggcgct gatttcggta
    28441 agctggcgat tgctcattct gccgaccagc aggcgctgaa cggcggccag atgggctggg
    28501 gccgtattca ggagttgccc gggatcttcg cccaggcatt aagcaccgcg aagaaaggcg
    28561 acattgttgg cccgattcgt tccggcgttg gcttccatat tctgaaagtt aacgacctgc
    28621 gcggcgaaag caaaaatatc tcggtgaccg aagttcatgc tcgccatatt ctgctgaaac
    28681 cgtcgccgat catgactgac gaacaggccc gtgtgaaact ggaacagatt gctgctgata
    28741 tcaagagtgg taaaacgact tttgctgccg cagcgaaaga gttctctcag gatccaggct
    28801 ctgctaacca gggcggtgat ctcggctggg ctacaccaga tattttcgat ccggccttcc
    28861 gtgacgccct gactcgcctg aacaaaggtc aaatgagtgc accggttcac tcttcattcg
    28921 gctggcattt aatcgaactg ctggataccc gtaatgtcga taaaaccgac gctgcgcaga
    28981 aagatcgtgc ataccgcatg ctgatgaacc gtaagttctc ggaagaagca gcaagctgga
    29041 tgcaggaaca acgtgccagc gcctacgtta aaatcctgag caactaatgg ttaaaaccca
    29101 acgtgttgtg atcactcccg gcgagcccgc cgggattggc ccggacttag ttgtccagct
    29161 tgcacagcgt gagtggccgg tcgaactggt tgtttgtgcc gatgccactc tccttaccga
    29221 ccgggcagcg atgctcggtt tgccgctcac cctccgccct tattccccca actcccctgc
    29281 acaaccgcaa actacgggca cattaacgct acttcctgtc gcgctacgtg aatctgtcac
    29341 tgcggggcag ttagcgattg aaaatggaca ttacgtggtg gagacgctgg cgcgagcgtg
    29401 tgatggctgt ctgaacggtg aatttgctgc gctgatcaca ggccccgtgc ataaaggcgt
    29461 cattaacgac gcaggcattc cgtttaccgg tcataccgag tttttcgaag agcgttcgca
    29521 ggcgaaaaaa gtggtgatga tgctggcgac cgaagaactt cgcgtggcgc tggcaacgac
    29581 gcatttaccg ctgcgcgata tcgcagatgc tatcacccct gcacttttgc acgaagtgat
    29641 tgctattttg catcacgatt tgcgtacaaa atttggtatt gccgaaccgc gcattctggt
    29701 ctgcgggctg aatccgcacg cgggcgaagg cggtcatatg ggtacggaag agatagacac
    29761 cattattccg gtgctcgacg agctgcgggc gcaggggatg aaactcaacg ggccgctgcc
    29821 tgccgatacc ctgtttcagc cgaaatatct cgataacgcc gacgccgtgc tggcgatgta
    29881 ccacgatcag gggcttcccg tgctaaaata ccagggcttc gggcgcggtg tgaacattac
    29941 gctgggcctg ccctttattc gcacatcagt ggaccacggc accgcgcttg aactggcggg
    30001 acgtggcgaa gccgatgtcg gcagttttat tacggcgctt aatctcgcca tcaaaatgat
    30061 tgttaacacc caatgaataa tcgagtccac cagggccact tagcccgtaa acgcttcggg
    30121 caaaactttc tcaacgatca gttcgtgatc gacagcattg tttctgccat taacccgcaa
    30181 aagggccagg caatggtcga aatcggcccc ggtctggcgg cattgaccga gccggtcggc
    30241 gaacgtctgg accagctgac ggtcatcgaa cttgaccgcg atctggcggc acgtctgcaa
    30301 acgcatccat tcttaggccc gaaactgacg atttatcagc aggatgcgat gacctttaac
    30361 tttggcgaac tggccgagaa aatgggtcag ccgctgcgtg ttttcggcaa cctgccttat
    30421 aacatctcca cgccgttgat gttccatctg tttagctata ctgatgccat tgccgacatg
    30481 cactttatgt tgcaaaaaga ggtggtgaat cgtctggttg caggaccgaa cagcaaagcg
    30541 tatggtcgat taagcgtcat ggcgcaatac tattgcaacg tgatcccggt actggaagtt
    30601 ccgccgtcag cctttacacc accacccaaa gtggattccg ccgtcgtgcg cctggttcct
    30661 catgcaacga tgcctcaccc ggttaaagat gttcgtgtgt tgagccgcat caccaccgaa
    30721 gcctttaacc agcgtcgtaa aaccattcgt aacagcctcg gcaacctgtt tagcgtcgag
    30781 gtgttaacgg gaatggggat cgacccggcg atgcgagcgg aaaatatctc tgtcgcgcaa
    30841 tattgccaga tggcgaacta tctggcggag aacgcgcctt tgcaggagag ttaacgatga
    30901 tcaattcgcc ccgagtgtgt attcaggttc aaagcgtcta cattgaggca caatcttcac
    30961 ctgataatga acgttacgtt tttgcttata ccgtaaccat acgcaatctg gggcgagcgc
    31021 cagtgcagtt gttggggcgt tactggctga tcaccaatgg caatggccgt gaaaccgaag
    31081 tccagggcga aggagtggtt ggcgtccagc cacttatcgc gcctggcgaa gagtaccagt
    31141 acaccagcgg tgcaatcatt gaaaccccgc tgggcaccat gcagggtcac tacgaaatga
    31201 tcgatgaaaa tggcgtccct ttcagcatcg acattcccgt attccgactc gccgttccca
    31261 cactcattca ttaaaagaat atggcgacat accttattgg cgacgttcat ggttgttacg
    31321 atgaactgat cgcattgctg cataaagtag aatttacccc tgggaaagat accctctggc
    31381 tgacgggcga tctggtcgcg cgcgggccgg gttcgctgga tgttctgcgc tatgtgaaat
    31441 ccttaggcga cagcgtacgt ctggtgctgg gcaatcacga tctgcatctg ctggcggtat
    31501 ttgccgggat cagccgcaat aaaccgaaag atcgcctgac accgctgctg gaagcgccgg
    31561 atgccgacga gctgcttaac tggctgcgtc gccagccttt gctgcaaatc gacgaagaga
    31621 aaaagttggt gatggcccac gcagggatca cgccgcagtg ggatctacag accgccaaag
    31681 agtgcgcgcg cgatgtagaa gcggtgctat cgagtgactc ctatcccttc tttctcgacg
    31741 ctatgtacgg cgatatgcca aataactggt caccggaatt gcgggggctg ggaagactgc
    31801 gttttatcac caacgccttt acccgtatgc gtttttgctt tccaaacggt caactggata
    31861 tgtacagcaa agaatcgccg gaagaagccc ctgccccact gaaaccgtgg tttgcgattc
    31921 ctggccctgt cgctgaagaa tacagcatcg cctttggtca ctgggcatcg ctggaaggca
    31981 aaggtacgcc ggaaggtatt tacgcgctgg ataccggctg ctgctggggt gggtcattaa
    32041 cctgcctgcg ctgggaagat aagcagtatt ttgtccagcc gtcgaaccgg cataaggatt
    32101 tgggtgaggg agaggcggtc gcgtcttaac gaattaccat tcagggagga ataatggctg
    32161 actattttga aatacgtgct gattacaatc agcacaccat cacgatttat caggcttaca
    32221 atgatgcaat cgctgacgtc gccgtacgtg acgggcgatt tggcgcacct ttttcattca
    32281 accgcatgac ctggattaaa ccctctttta tgtggatgat ggagcgtagc aactggggac
    32341 tgaaaaaaga tcaacaacac attctcgcaa tccgaatcaa acgcaccttc tttgatacat
    32401 tgctggaaca agcggtgcta accacgccgg aagcccatgt gtatccacat gcaggaattt
    32461 gggaaacgtt atttgcacaa gcaaatgtgt atgttcagtg ggacccagaa cgcagcataa
    32521 atggtaaaaa actggagcac cgatcattac agcttggtat ttctcgtaac ttgatcagtc
    32581 aatttaacga agacgcgatt gttgctattg atgatctgac gccgctggtg cgaaagtgtc
    32641 ataacctttt gataaacggc aaaacaacac aggcgaaatc cttcctgccg ccagaaaaaa
    32701 tttacccagt tagtgcagca gcgagaaagg ctttgggaat gaaaaattcc tgagacaaat
    32761 acgcacctat taagaggaaa atatctaagt gcgacaaatc aacaccgggc ttaataccag
    32821 agaaattatt ctggaaatga ttgacgaagc agatgccccc ccgtttgctg agcagcggac
    32881 ctggcatcgc aggcgaatcg cttttctatg tgattgcatc agcgttgctg tcgactacgg
    32941 cttcttctgt tctgtctctc agcagtcaat aatgaacata accaagatgg ctgaagatgt
    33001 atggataaca gaaagtaaaa cgacccaact cattgatatt tggcaacaac ttaaacagct
    33061 ttatccgcca gaaacaattc cctacgccca gaagttaccc actaaggaat tgcttgaact
    33121 gctactttct agtcctgacg atcttcgaat aattgaaatt cctgacatca tcggtgatgt
    33181 ttttgatgac caaacctgga ttcgattatt caaaaaacac tttgctgatg ttattcagct
    33241 acgatgtgat gtgtaacaat ccaaaatata tcgccagacg atagaccaaa aagaaagtat
    33301 ttagcagata ctcaacattc tgacttctgt acagaaatag cagagtttaa aaacttaaaa
    33361 cttccggtac tcatcagaga aacatccagt ttcttctgca aattcattta atgccgagat
    33421 ggcatcctcg tttacttcat tccactgtag agtggcttga aggtttagac ctacgcaatg
    33481 agcgcgactc acaaaatcgc gctcactggt aaaatcaaac gtcttatcgt tcaatttgta
    33541 gtcctggcaa aaaattaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc
    33601 gcatcggcgt cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg
    33661 aaatgggtgt cgccttccac ttctgcgtcg atatgcgtca gatacagttt ttgcgctttt
    33721 ggcaagaact gttcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca
    33781 cacgccgcga tggcttcatc aaccgatttc acccacgtta cgcgatcgtc cgtacccggt
    33841 tgactgctga ggataatatt tttgcgtcct ggcagcggac gaccgatgga ttcccaggta
    33901 tggcggccca taataacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca
    33961 ggcaggttcc acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca
    34021 atcagactga tcattgagat ttcccgataa aaaaaattgt cgccactata cgtaaagcgt
    34081 aaaccgtcgt cgactggtgc gaggatgatg ttgaggaaaa ttttatattc tgctggcgag
    34141 tccacgctct ctccctggac tcgccacatt acaatgaaac aaaaacaaac agttagctgt
    34201 aaagtgtgat ttacgtcact ctttattagg atgagggttt cgtttccggt tcatccgcca
    34261 tgttgccggt atgtttacct tcttctgttc cctgccagcc atgtcgctga attaatgaca
    34321 gatgttcgcg gtcctcggta atgatctcac ttaacatcgc gctggtgcgt ttatagaccg
    34381 ccgcgcgggc tttggtgtca ttctcaacca ttgccatctc ttccaccatc tgaatattaa
    34441 agcggcggaa cacatcggca cgttctcgcg cttcatacgg ccccagacct aaactttcca
    34501 gcgccagacg cccggttttc agcgcacctt caaaggtttc acgctccggc ttttcaacgc
    34561 ctgcctgacg caaacgaatg tagtggtcga catcgcgggc gcgggcaata atctgcaaat
    34621 gcgggaaatg ttctttcacc atctctgtca gttgcaggtt ggtttgcgga tcgtcgatgg
    34681 cgttaatcag cacttccgct ttcgccgctc cggcagattc cagtaaatcc atccgcgtgg
    34741 catcgccata aaacactttc ataccaaatt tacgcaaggt ttcgatatgg tccggatcgt
    34801 gatcgagtac caccattttc accccgctgg agagcagtaa acgtccggta atctgcccaa
    34861 aacgaccgaa tccggcgata atcacgcgcg gctgttcttc gtcgatctca tcggcttcac
    34921 gcgcttcctc agtagaagat tgctcaaggc gattgaggat caccagcaga atcggcgttg
    34981 ctgccatcga cagcgccacc gccagggtca gcgatttcgc ccactccggc tccagcacat
    35041 tcgccatctg cgccgcgcca aataccacaa aggcaaactc actgccctgc cctaacaaca
    35101 ccgcaaacca acgacgctgt ttatttggca cttgcaacgg tcgggcaatc agccacagca
    35161 tggcgatttt gatgatgagg aaaccgagca gcaaaatgac aatgcgcaat gggttttcaa
    35221 gcagcgtgcc aaagtctatc gacatgccaa caccgatgaa aaacagcccc aacagcaaac
    35281 ctttaaatgg ttcgatatcg ctctccagcg catgacggta ttccgagctt gccagcagta
    35341 cgcccgccag aaacgcgccc atcgccatcg acaagccgac ctcttccagc agcaaaccaa
    35401 agccaaacac gaggaataac gccacggcac taaacacttc ccgcaagcca gagcgggcta
    35461 caaaacgcag cgccggacgc gtgacatagc gccccagcaa taccaccagc accagcgcac
    35521 ccgccacttt taacgccgag agagcaaatg cgcccatcgt cgtcgaggca ctgctcgttg
    35581 ccagtagcgg aatcatcgcc accagcggga tcgccgcgat atcctggaac agcagcaccg
    35641 caaaggcact gcggcccatt tgcgtcacca tcagattgcg ttcattcatc gcctgcatgg
    35701 caatcgccgt tgatgagagc gccagcgtca tgccgatcag ttccgcgacc tgccagcgca
    35761 gcccaagtaa catgcagaac agccccagca ggccaccgca aatcaccatc tgcaatgcgc
    35821 cgccgccgaa cactgccgca cgcagcttcc atagcctttg tggatcgagt tcgaggccga
    35881 taataaacag catcagcacc acgccaatct cggcaaagtg caggatagat tcggcatcgg
    35941 tcaccagtcg cagcccccac gggccaataa tgcaaccggc aatcaggtag ccaagtaccg
    36001 atcccagacc aagacgtacc gcaatgggta caatcagcgc cgccgatccg aggtagatca
    36061 gcgcctgaac cagcgtatgg ctatccatga tgggcctcct gccattccag cagacgttgc
    36121 ttatagtgac gcgcctgccc ttcgagggtt tcgtcgtcac aaataaaggt gcagtgcatg
    36181 gcaaacggtg gcagccagtt cagcccgcag tagattgccg tcgcctgtag cggctgcgac
    36241 agcacatcaa agcccggatg cgcaccaatt tcaaaatggc tttccccgcc gccggtcgtc
    36301 accgcccaca gcaaatgttt gccatgcagc gccgtgccgc cgtgaccgta agcccagccg
    36361 tgcgagaaaa ctttatcgat ccaaagtttg aggagcggtg gaatgctgta ccactgcatc
    36421 gggtgctgcc agacgatcag atcggcgcga gacagcgcct cctgctcggc ggcaatatcg
    36481 atattgaagt caggatagag ttgataaaga gagcgaattt cgacgccttc cagcgtcctt
    36541 gcctgttcaa gcatccgttt attcgcatgg gaatgatgcg gatacggatg cgcataaatt
    36601 ataagaatca taggtagccc gcgtgattac gcctttgttt tcccacaaag tgtagtcata
    36661 tattcacgcg gcttatagtc aataatattg attcagaaaa gagagaaatc tgaacaaatt
    36721 accccattct ctgcccgcgg atcgtcatat tgctggtctg cgccagtgac atccctcgag
    36781 tctccggggc aaacgctacg gaaatcaaca agccaaacag cgagataccc gcccccatta
    36841 gcatcgtgtt actgataccg taattattga taaagatcgg cagtgcccaa gtcgaaacaa
    36901 tggtgccaat acgacttaag gacataatca cgcccacggc agaggcgcgg atatccgtcg
    36961 ggaagagttc attaggatag agccactgca aattacccgg cccgccagag aaaaaggcat
    37021 acaccgcaaa ggccatcact accagccaga tccccatatc cgggatcagc cccaaaaccg
    37081 ccagcgccag cgtcatcatg gcaaagctgc caatcaacaa tggacgccgt ccggcagtgt
    37141 ttagccacag catcggcgga atacagccga gcataaagaa caggctaatc accacattcc
    37201 ccagtgccgc gtttttgcca acccccaatc ccaacaaacc aacgatttgc gggccaaagg
    37261 tgtagatggc gaacatcggg atcacctggc aagtccagat ggcggcaaca aacagaacaa
    37321 aaggaaaatg gcggcgatta aacagatcgc gaaaacgggt ttgctgcggc tgctcttcct
    37381 cgaaagccac cggttcgcca aacagtttta tcatcatctc ttcgcactct tttactcgcc
    37441 ctttgcgtaa taaccagcgg ggagattcag gcagttcgaa tcgaccaatc aaaatcaaca
    37501 aacaggggat cgccgcgcta cccagcatcc agcgccagcc gccttccaca tcataaagcc
    37561 agtagccgac cagatcggcg caggtcgcgc ctacatacca catggcggcg ataaagctga
    37621 tggaaaatgc ccgctgacgg gtactggaga actcggtgat cattgaggtg gcgatgggat
    37681 aatctgcacc gatgacaatg ccgataagta cccgcatcac caacagttcg acgggggatg
    37741 aaacaaacat cgtcgccacc gatatcacgc cgatggcgat gatatcaatg aggaacattt
    37801 tgcgccgtcc gactttatcg gaaatatagc caaacagcga tgtgccaacg aacagcccgg
    37861 cgagcgttcc cgcgcccaac aagccaatcc agtcagcgtc cagttttagc gccggagtca
    37921 gttgctccag cgccacgcca atcattacca gtacataacc atccagaaac ggaccaccgc
    37981 ttccccacag caaaatgcgg cggtgaattg aggagaattt gagatcgtca aagtttctgg
    38041 acggttgcat gggttattcc tgtttttttg caatgagagt cagtacggcg ttacgtatca
    38101 aaccaacatc agccgtaacg gaactccaca ccaaaggtgc cgcgcgggta ttcccactgt
    38161 tccagcgccg accccagccc cagaatgcga caggtgccgc actccagaca tccggcgtaa
    38221 tcgaagcgca cactgccgtc atcctgcttc ttgtacagac ctgcggggca cactttcacc
    38281 agcagctcca gggcctgttt atcggcatcg gccttcacaa caatgtgcgg atgctcttca
    38341 tcgacattga atttattgac gcccagtttg acgtccacat tgacgggaga agtcataaaa
    38401 cggtcactcc tttcatgcca tccttgatca gattgatgaa gcccactttc ttgccgtggc
    38461 ggaggatttt cttgcgcatc agttccggcg cactgccgtc aatggtgaac aggtcacgcg
    38521 ccacgcccac cgccagttcc gggtagccgc taaacatgcg tgggttatca aggaaggccg
    38581 gtagtttctg gtacatacgc atatcgcgca gcgggccact ctcaagatgc tgacgatatt
    38641 ccgcgagttt ttgcttactg aaatcgtcgc ttttcatcgc tgaaagcacg gtttttgctg
    38701 cagcttcccc ggcggcaatc gccagatcca taccgcgaat ggtaaaaccg aggttcatac
    38761 acattccggc ggcatcaccg gcaatcaata cgccgtcacc gaccagctcc ggcagcatgt
    38821 tgatgcctgc ttccggcact acgtgagcgg aatattccac cagcttgccg cccgcgatca
    38881 gcggtgccac tgccggatgc tgtttgaaat cttccagcat ttgcggcacc gatttttttg
    38941 cgtcatgcag atgatgcaga ccacaaacca gccccagcga cagggtattt tcattggtat
    39001 aaaggaagcc gccgcccatc aggccatcgg tgggtgatcc cgcaaacaga caagccgcgc
    39061 cctgattacc ctgcaactga aaacggtctt cgattaccga cttcggtaac tcgatcagtt
    39121 ccttcacgcc aaccgccaca tccgtcggtt taacgcgttt tgccatcccc aacttttcgg
    39181 caaggatgga gttcacccca tcagcaagga tcaccgtttt cgcttcaatc acatcgccat
    39241 cggcttctac accgacgact ttgccatcgc gctgtacgag gttatcgaca cgaatcccgg
    39301 taattaactg cgcgcccgct tcttcggcct gctccatcaa ccaggcatca aatttactgc
    39361 gcaaaacgga gtaagaacgc tgggatggcg aggtttcgtc accgttgcag tagtccatag
    39421 tcatcgctga cttttccgtc ataaacgcga gtttttcatg ggtgatcagg cgttctacgg
    39481 gggcagattc ggcgaaacca ggaataatgt gttccaggct gtgggcatag agacgcccgc
    39541 cggtgacgtt cttggcacct gcggaattgc cacgctcgat aactaacact tgcgcccctt
    39601 cgcgggcgag caccagtgcg gcaaccgaac cggcaagccc agcaccgacg atgatggcgt
    39661 caaagatatc ttcggacata agagctccct gtcagcggca gggccaaaat gcagccctgc
    39721 cagagtggat caacgcgcta aagctgcggt cagcgccgga aggatcttca cggcgtcgcc
    39781 aacaatgccg taatccgcgt actggaagat cggcgcattt ttatctttat tgatggcgaa
    39841 aatggtttgc gacgcgttag cgccaaccat gtgctggatc tgcccggaga tccccaccgc
    39901 caggtacagt tcaggtttca gcatcaggtt ggagataccg acatagcgtt cgtgctccat
    39961 ccatttttcg ttttctgcca ccggacgaga acaggccaac tccgcaccta tcgccttgca
    40021 aagctgttct gccagcgcaa tgttctcttt gctgccaata ccgcgaccga cgctgaccac
    40081 caggcgggct ttgtcgagat cgacgctgtt gctctggcgc gcctgggttg ccgtgcgggt
    40141 aatcgccaca gccggagcct gccactccac ggtgtgcgtt tcgccagtgc gtgacgcgtc
    40201 tggctgagcc gcatcgaacg tgccgctgct gatggtcagt accgcatacg gcgtggcaat
    40261 gcgttcttcg ccaatcgcca gaccaccgta aaccatgtgt ttcactgtcg ctttaccgtc
    40321 ctgtacgctg acggtgctgg catcgttaga caccgccgct ttaaggcgat aacccagttt
    40381 tgccgccagt aatttgccgc gacgggtgtt tggcagcagc accaggccgt ctgcgccgtg
    40441 ctggcgaata gtgtcagcca tgacaccggc gtaatcttcg atcatccgat cgtccggttt
    40501 gccgtttaat ttccagacat gattagcgcc gagctggatt gcctgtgcgc cgtcggcatc
    40561 attgaggaca aaggtgttga tttgattagc taaagcctgc gcaccgttca tcagttccgg
    40621 cagacgagaa ggggtatcgc tgaataccca gacttgagaa aacgtgttca tagcatcccc
    40681 tgtaattaaa tgactttgcg aagattttca gcaaatgcgg cgatctgttc ttcgccgtcg
    40741 ccttcaatca cgatgcgctg acgttcgcgc tgtttcggcg cggcaacctg ttgttctgac
    40801 caggctgcct ctgcgttaaa accaatatcc gccgccgacc atacctggac gggctttttc
    40861 gccgcgccga gaatggcttt catcgaagga atttgtgggg aattgatatc agtggaaaca
    40921 gcaacaaccg caggcagcgg aatgcttaag gtttcggttt catcttccag ttcgcgctca
    40981 acggtgaggg tatctgcagt cagggagata attttgctga cgccgttaac tgccggaata
    41041 ttgaggattt cgcccaccag cagaccaacc tgctgggcat aaaggtcgga agaaccatcg
    41101 ccacagagga tcaaatcaaa gcctgctttc tgggcggctg cagccagtgc gctcgccgtt
    41161 tgttgcggca gtgcctgctc gaactggtca tcaatcacca caatcagttc atccgggccg
    41221 cgcgatagca catctttacg ccctttggcg ttggtcaggg ctttaccgcc cacacttaag
    41281 gccgtcacct gcgcctctgc tgcctgttgc tttagctggc aagccgcttc aatagcgttc
    41341 agatcgtatt ggcttatttt ggcatcggct ttgctgaagt ctaatgaacc atcagcatta
    41401 ttgaccgcaa tatcctgttc atcaggcacg cacttatagc aagtaataat cttcattgca
    41461 tctccagaaa tcatgaagga aacgtcgttc atatcggtgg tgttaattac agatatcacg
    41521 ccctttaatt tggaaataaa actttaatca ccaatattga aaatgtcaca cgcagataaa
    41581 tccaactttc aatattgtta agttcctcac caatattgaa aacatggcgt agcaaaaaga
    41641 aattttcaat attgctttat ggatcaccaa tattgaaagt cagatgctat ttttttactc
    41701 acaacacaac ataacaaact gattaataat caaaattaac gagaaaagcg ccctctattc
    41761 acccgaaaca aaaatgtgat aacaatcaca gaatgcagct tattgaatac ccattatgag
    41821 ttagccatta acgcgtccac gaggttaata ataattatat taaatgttaa caaaaataaa
    41881 acaaacggga acgcaaaata aatattcgtt ttcacagtgg aattaactca tgaagaatga
    41941 aaagagaaaa acgggaatag aaccgaaggt tttctttccg ccgttaataa tcgtcggcat
    42001 actttgttgg cttacagtca gagatctgga tgcagcgaat gtcgttatta atgctgtatt
    42061 cagttacgtc accaatgtat ggggatgggc atttgaatgg tatatggtgg tgatgctttt
    42121 cggttggttc tggctggtgt ttggcccgta tgccaaaaag cgtttaggta acgaaccacc
    42181 tgaatttagt accgccagtt ggatctttat gatgttcgcc tcctgtacgt ctgctgccgt
    42241 actgttctgg ggatcgattg agatctacta ctacatctcc accccgccgt ttggcttaga
    42301 accgaactcg acaggggcga aagagttggg gctggcttac agcttgttcc actggggacc
    42361 tctgccgtgg gccacttaca gcttcctttc agtcgccttc gcttacttct tctttgtccg
    42421 caaaatggaa gtgattcgcc ccagctcgac actggtgccg ctggtaggtg aaaaacacgc
    42481 caaagggttg ttcggcacta tcgtcgacaa cttctatctc gtcgccttga tcttcgcgat
    42541 gggtaccagt ctgggccttg ccacgccgct ggtgaccgag tgtatgcaat ggttgtttgg
    42601 cattccgcat accctgcaac tggacgctat catcattacc tgctggatta tcctcaacgc
    42661 catttgcgtc gcttgcggtc tgcaaaaagg ggtacgtatc gccagtgacg tgcgtagtta
    42721 cctgagcttc ctgatgctgg gttgggtgtt cattgtcagc ggtgccagct tcatcatgaa
    42781 ctacttcacc gattcggtgg ggatgttgct gatgtatctg ccgcgcatgt tgttctatac
    42841 cgatcccatc gctaaaggcg gcttcccgca gggctggacc gtgttctact gggcatggtg
    42901 ggtgatttat gctatccaga tgagtatctt cctcgcccgc atctcccgtg gtcgtactgt
    42961 gcgtgaactg tgcttcggca tggtgctggg gctgacagcg tcaacctgga tcctgtggac
    43021 tgtactcggt agtaacactc tgctgttgat agataaaaac atcatcaaca ttccaaatct
    43081 gatcgaacag tacggtgtgg cgcgcgccat cattgaaacc tgggccgctc tgccactcag
    43141 caccgccacc atgtggggct tcttcatcct ctgctttatt gccaccgtta cgctggttaa
    43201 cgcctgctct tataccctgg cgatgtccac ttgccgcgaa gtacgcgatg gtgaagaacc
    43261 acctctgctg gtgcgtatcg gttggtcaat tctggttggc attatcggta ttgttctgct
    43321 ggcgctcggc ggcctgaaac cgattcaaac cgccattatc gccggaggat gcccgctgtt
    43381 cttcgtcaac attatggtga cgctctcctt tattaaagac gcgaaacaga actggaaaga
    43441 ttaattaacc cccaaaatat caagaggttg aaagatggat tttaatttaa atgatgagca
    43501 ggaactgttt gtcgccggta tccgcgaact gatggccagc gaaaactggg aggcctattt
    43561 tgccgagtgc gaccgtgaca gcgtctaccc ggaacgtttt gtcaaagcac tggcggatat
    43621 gggtatcgac agtctgctga tccctgaaga gcacggtggt ctggacgcgg ggtttgttac
    43681 tctcgccgcc gtgtggatgg agctgggacg tctgggggca ccaacctatg tactgtacca
    43741 gttgccgggc gggttcaaca ccttcctgcg cgaaggcaca caagagcaga tcgacaagat
    43801 tatggctttc cgcggcaccg gtaagcagat gtggaactca gcgattaccg aaccgggcgc
    43861 gggctccgac gtgggtagcc tgaaaacgac ttatacccgt agaaatggta agatttatct
    43921 taatggtagt aagtgtttta ttaccagcag cgcctacacc ccgtacatcg tggtgatggc
    43981 gcgcgacggg gcttctccgg acaaacctgt ctacaccgaa tggtttgttg atatgagcaa
    44041 accgggcatc aaagtgacca aacttgaaaa gctcggtctg cgtatggata gctgctgtga
    44101 aatcaccttt gacgacgtgg aactggacga gaaagacatg ttcggtcggg aaggtaacgg
    44161 ctttaaccgc gtcaaagaag agttcgacca tgaacgtttc ctggtagccc tgaccaacta
    44221 cggtacggcg atgtgcgcct ttgaagatgc ggcgcgctac gccaaccagc gcgtgcagtt
    44281 tggcgaggct attggtcgtt tccagttgat tcaggaaaaa ttcgcccaca tggcgatcaa
    44341 attaaactcc atgaaaaaca tgctgtatga agcagcgtgg aaagcagaca acggcaccat
    44401 cacctctggc gatgcagcga tgtgcaaata cttctgcgcc aatgcggcat ttgaagtggt
    44461 ggatagcgca atgcaggtgc tgggcggtgt cgggattgcg ggcaaccacc gcatcagccg
    44521 cttctggcgt gacctgcgtg tagaccgcgt ctccggggga tctgacgaaa tgcagatcct
    44581 gacgctgggt cgtgcggtgc tgaagcaata ccgctaagtc aatgcgctgc ctgatgcgac
    44641 gctgatgcgt cttatcaggc ctacgggagc acgaatgtag gccggataaa gcgtttacgc
    44701 cgcatccggc agtcatgcgc tacataaatt tttcaggagc aaataatgga tcatctaccc
    44761 atgccgaaat tcgggccgtt ggccggattg cgcgttgtct tctccggtat cgaaatcgcc
    44821 ggaccgtttg ccgggcaaat gttcgcagaa tggggcgcgg aagttatctg gatcgagaac
    44881 gtcgcctggg ccgacaccat tcgcgttcaa ccgaactacc cgcaactctc ccgccgcaat
    44941 ttgcacgcgc tgtcgttaaa tattttcaaa gatgaaggcc gcgaagcgtt tctgaaatta
    45001 atggaaacca ccgatatctt catcgaagcc agtaaaggtc cggcctttgc ccgtcgtggc
    45061 attaccgatg aagtactgtg gcagcacaac ccgaaactgg ttatcgctca cctgtccggt
    45121 tttggtcagt acggcaccga ggagtacacc aatcttccgg cctataacac tatcgcccag
    45181 gcctttagtg gttacctgat tcagaacggt gatgttgacc agccaatgcc tgccttcccg
    45241 tataccgccg attacttttc tggcctgacc gccaccacgg cggcgctggc agcactgcat
    45301 aaagtgcgtg aaaccggtaa aggcgaaagt atcgacatcg ccatgtatga agtgatgctg
    45361 cgtatgggcc agtacttcat gatggattac ttcaacggcg gcgaaatgtg cccgcgcatg
    45421 agcaaaggta aagctcccta ctacgccggt tgcggtctgt ataaatgcgc cgacggctac
    45481 atcgtgatgg aactggtggg cattacccaa attgaagagt gctttaaaga tattggcctc
    45541 gcacatctac tcggtacgcc ggaaatcccg gaaggcactc agcttatcca ccgtatcgaa
    45601 tgcccttacg gcccactggt agaagaaaaa ctcgatgcct ggctggcggc acataccatc
    45661 gcagaagtta aagaacgctt tgccgaactg aatatcgcct gtgccaaagt actgaccgta
    45721 ccggaactgg aaagcaatcc acagtatgtt gcccgtgaat caatcactca gtggcaaacg
    45781 atggatggtc gcacctgcaa agggccgaac atcatgccga aattcaaaaa taaccccgga
    45841 caaatctggc gcggaatgcc ctcacatggc atggacacgg ctgccatttt gaaaaatatc
    45901 ggctacagcg aaaacgacat tcaggagttg gtcagcaaag gtctggccaa agttgaggac
    45961 taaacgcgtg gcgtcagtcg gcttgcctga ctagcgcccg cccaataaca aacgaacaaa
    46021 aatggataga ggtgcaatgg atatcattgg cggacaacat ctacgtcaaa tgtgggacga
    46081 tcttgcggac gtttacggtc ataaaacggc gctgatttgt gaatccagcg gcggagtcgt
    46141 taaccggtat agttatcttg agttaaatca ggagattaac cgcacggcaa acctgtttta
    46201 tacgctaggg attcgcaaag gcgacaaggt tgctctacat ctcgacaact gcccggaatt
    46261 tatcttttgc tggttcgggc tggcaaaaat tggcgcgatt atggtgccga ttaacgcccg
    46321 cctgttacgc gaggaaagcg cgtggatcct gcaaaatagc caggcgtgcc tgctggtgac
    46381 cagtgcgcaa ttctatccta tgtatcaaca gattcagcag gaagatgcca ctcaattgcg
    46441 gcacatttgc ctgacagatg tggcacttcc cgctgatgat ggcgtgagtt cgtttactca
    46501 actgaaaaat caacaacctg ccaccttgtg ctatgcaccg ccgctatcga ctgacgatac
    46561 ggcggaaatt ctcttcacct ccggcaccac ctcccgaccg aaaggtgtgg tgattaccca
    46621 ttacaacctg cgcttcgctg gatattactc cgcctggcag tgtgcactgc gtgacgatga
    46681 cgtctacctg acggtaatgc ctgcgtttca tatcgattgc cagtgtactg cggcgatggc
    46741 ggcgttttct gccggggcca cctttgtgct ggtcgagaaa tacagcgccc gcgccttctg
    46801 gggacaggta cagaagtacc gcgccaccgt taccgaatgt attccgatga tgatccgtac
    46861 cttgatggtg cagccgcctt cagcgaacga tcggcaacac cgcctgcggg aagtgatgtt
    46921 ttatctcaac ttgtcggagc aggaaaaaga tgcgttttgt gaacgcttcg gcgttcgctt
    46981 gctgacgtct tatgggatga cggaaaccat tgttggcatt atcggcgatc gccctggcga
    47041 taaacgacgc tggccgtcga ttggtcgggc ggggttttgc tacgaagcgg agatccgcga
    47101 cgatcacaat cgcccgctcc cggcaggtga gatcggtgaa atctgtatta aaggcgtacc
    47161 agggaaaacc atcttcaaag agtattttct caacccgaaa gccactgcga aagtgctgga
    47221 agccgacggc tggctacata ccggcgatac cggatactgc gacgaagagg gcttttttta
    47281 tttcgtcgat cgccgctgca atatgatcaa acgcggtggc gagaatgtct cctgcgtgga
    47341 gctggaaaat atcatcgcca cccatccaaa aattcaggat atcgtggttg tgggtattaa
    47401 agattcgatt cgcgatgaag ccatcaaagc atttgtggtg ctgaatgaag gtgaaacatt
    47461 gagcgaagag gaatttttcc gcttctgcga acaaaatatg gcgaaattta aagtgccctc
    47521 ttatctggag atcagaaaag atctgccacg taattgctcg gggaaaataa ttagaaagaa
    47581 tctgaaataa atgaaacagc agggaactac cctgccagct aataatcacg ctataaaaca
    47641 atatgcattt tttgcaggga tgttgtcgtc cctgaaaaag caaaaatgga gaaaaggaat
    47701 gagtgaatca ttacatctga cccgcaatgg atcaattctg gaaattaccc ttgatcgtcc
    47761 aaaagcgaat gctattgatg caaaaaccag ctttgaaatg ggcgaagtat ttctaaattt
    47821 ccgtgacgat ccgcaattac gtgtcgccat tattaccggt gccggagaga agttcttttc
    47881 cgcgggctgg gatttaaaag cggcagcaga aggcgaagca ccggatgctg actttggccc
    47941 gggtggattt gcgggattaa ccgaaatttt caatctcgac aaaccggtta tcgcagctgt
    48001 gaacggctat gcctttggcg gcggtttcga actggcgctg gcggcagatt ttattgtttg
    48061 tgccgataac gccagcttcg ccctgccgga agccaagctg ggcatcgttc ctgacagcgg
    48121 cggcgtgctg cgtctaccga agatcctgcc gcctgccatc gtcaatgaaa tggtgatgac
    48181 cggcagacga atgggcgcag aagaggcgct gcgttggggg atagtcaacc gcgtggttag
    48241 ccaggcggaa ctgatggata acgcccgcga actggcgcag cagctggtta acagcgcccc
    48301 gctggcgatt gcggcgctga aagagatctt ccgcaccacc agcgaaatgc cggtagaaga
    48361 agcgtatcgc tatattcgca gcggcgtgtt gaaacactat ccgtcggttc tgcattcgga
    48421 ggatgccatt gaagggccgc tggcgtttgc cgagaagcgc gatccggtgt ggaaaggacg
    48481 ttaacaaccg tgagctatta cgcctttgag gggttaattc cggtggttca cccgacggcg
    48541 tttgtccatc ccagtgccgt cttgattggc gatgtgattg tgggagccgg tgtctacatc
    48601 ggcccactcg cctcactgcg tggtgactac gggcggttga tcgtgcaagc gggagccaat
    48661 attcaggatg gctgcattat gcatggctac tgcgacactg acaccatcgt tggggaaaac
    48721 ggccatatcg ggcacggagc gatcctgcat ggttgtgtga ttggtcgcga tgcattggtc
    48781 gggatgaaca gcgtgattat ggatggcgcg gtcattggcg aagagagcat tgttgccgcc
    48841 atgagctttg tcaaagcagg ctttcacggc gagaaacgcc agttgttgat gggtacgcca
    48901 gcccgctctg tacgcagtgt tagtgacgac gagttacact ggaaacggtt gaataccaaa
    48961 gagtatcagg atcttgtcgg gcgctgtcat gcatcgttac atgaaacaca gccactgagg
    49021 caaatggagg aaaatcgccc ccgtttgcag gggacgacgg atgtgacgcc taaacggtga
    49081 tcttgccgga tgcgacctgg ttgctggata agatgctaca acatcgcatc tggcaagcag
    49141 gacgcccggt atcaacgaga taaaattaac gacgcatact ctttgacaac atcatctgcc
    49201 acttctgctc gcggttaagt tccgtgagcg gaacggcagg aatacgcggc gtctttctta
    49261 cgccgactaa tttatcccgg ctgttattgc gcaggcgcgc gtaaatttgc tcatcgatat
    49321 cgaccacttt taccagtcgc tggcactggc atccccgtcc ttccagctta ttagggatca
    49381 tcttaacttc gcagcttatt tctgcgactt ccgacagaat ataagtcagg gtattaaccg
    49441 ccttgctgtg ttcaatatcg aaatgaatag agatctctct tgctatcacc caccgatttt
    49501 cagccatcat ccattcggcg attaacaagt agagtggttt ttcaacatat ccttcacaca
    49561 tacgaactcc agtttcacaa attaaaagtc tggctatgac tcgttttgaa gcaattaaac
    49621 aaggccatat taaaattgtg gatatatcga tagtatgcaa cttcaccgtc gataagtgtg
    49681 agctaaatcc tgcttatgtc atcaaaaata tcgattcccc aaaagattta cttaatgggc
    49741 aaaaaaaacg gtcctcatca gagaaccgta ttacttattc gatcaaatta gcggatgaaa
    49801 aatatccgcc atgacacgct attatttgat ctgcgcgtgc atttcctgca ccgaaattac
    49861 tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg aaaccaccgt tcagggtggt
    49921 gtcataatgc actttatatt gcagcgcact gcgacggatc acgcgggagt cttcaatcgc
    49981 gcgacggcct gaagtggtgt tgatgatgta ggtatattcg ccattcttga tgcggtcctg
    50041 aatgtgcgga cggccttcat gcaccttgtt caccagacgc ggattaatgc ccgcttcgcc
    50101 cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag ccctgtttca gcagttttgc
    50161 cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg gaaagcagcg cacgaccgtg
    50221 tttcttcatg gtggagttgc tgcccagctg cgctttggca aacgcttcag cgaaggtgcg
    50281 gcccacgccc atcacttccc cggtggagcg catttctggc cctaacagcg ggtcaacacc
    50341 cgggaattta ttgaacggca gcaccacttc tttcaccgag tagtacggcg ggataacttc
    50401 tttggttacg ccctgctcag ccagcgattt gccagccatc acacgcgccg ccacttttgc
    50461 cagcgatacg ccggtggctt tggagacgaa cggaacggta cgcgccgcac gcgggttaac
    50521 ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc acgttcatca gaccgcgcac
    50581 ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc acatcctgaa tttcctgact
    50641 taaggtgtag gctggcagag aacatgcgga gtcaccggag tgcacgcccg cctgctcgat
    50701 gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg cagatggcat ccacgtcaac
    50761 ttctaccgcg tcatcaagga agtgatccag caacactggc gcatcgttag acacgctgac
    50821 cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag acgatttcca tcgcccgacc
    50881 gccgagaacg taagacggac gtaccaccag cgggtagcca atctctttcg ccttctctac
    50941 cgccatttca atagcggtaa cggtggcgtt cgccggttgt ttcagtttca gacgctcaac
    51001 cgcatgctgg aagcgttcac ggtcttctgc acggtcgata gcatccgggc tggtgccgat
    51061 aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc agcggggtct gaccgccgta
    51121 ctgaacgata acgcctttcg gcttctcaat acgcacgatt tctagcacat cttccagagt
    51181 taccggctcg aagtagaggc gatcggaagt gtcgtagtcg gtggagacgg tttccgggtt
    51241 acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc gccagcgagg cgtgtacaca
    51301 gcagtagtcg aattcgatac cctgaccgat acggttcggg ccgccgccga gaaccatgat
    51361 tttttcacgg tcggtagacg gattcgcttc gcactcttct tcataagtgg agtacatgta
    51421 agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg cgcttataaa ccgggtgcag
    51481 gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc acgcccgcca gttttgccag
    51541 acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg aattcagcgt tcaggccagt
    51601 gatgcccact tccgctactt tctcttccag acgcaccagt tcttcaatct gtaccaggaa
    51661 ccagcggtca atattggtca ggttgaacac gccgtccaca gacaggcccg cacggaaggc
    51721 atcggcgatg taccagatac gctctgcgcc agcatctttc agttcgcgac ggattttggt
    51781 taacgcttcc gggtcatcga ggctaacttt cgggtcgaat ccagtcgcac cgacttccag
    51841 gccgcgcagc gctttttgca gggattcctg ctgcgtgcga ccaatcgcca tcacttcgcc
    51901 aaccgatttc atctgagtgg tcagacggtc gttagcacca gagaattttt cgaagttgaa
    51961 gcgaggaatt ttagtaacca catagtcgat ggacggctcg aaggaggccg gagtacgtcc
    52021 gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc accgccagtt tcgccgccac
    52081 tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc gaagaacggg acacgcgtgg
    52141 gttcatttcg ataacaatca ggcgaccgtt tttcgggttc accgcaaact ggacattgga
    52201 accaccggtt tcaacgccga tttcacgcag caccgccatt gaggcgttac gcatgatttg
    52261 atattctttg tcggtcagcg tttgggctgg cgcgacagtg atggagtcac cggtgtggat
    52321 gcccatcgca tcaaagtttt cgatagagca gacgatgatg cagttgtcgt ttttatcacg
    52381 caccacttcc atctcgtact ctttccagcc gatcagcgac tcatcaatca gcagctcctt
    52441 ggttggggag agatccagac cgcgggcgca aatttcttca aactcttcgc ggttataagc
    52501 gataccgccg ccgctaccgc ccatggtaaa ggatgggcga ataatgcacg ggaagcccac
    52561 gtcagcggca accgccagcg cttcttccat cgtgtgtgcg ataccggaac gcgcggtttc
    52621 cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg tcttctgctt tatcaatcgc
    52681 atcggcggtc gcaccaatca tagtcacgcc gaactcttcc aacacgccct gacgctccag
    52741 ctccagcgcg cagttcagcg ccgtctgacc gcccatcgtt ggcagcaccg cgtccgggcg
    52801 ctctttttca ataatcttgc gtactacttc ccagtgaatc ggctcgatgt aggtcgcatc
    52861 ggccatttcc gggtcggtca tgatggtcgc cgggttggag ttcaccagaa tgacgcggta
    52921 accctcttcg cgcagggctt tacatgcttg cgcgccagag tagtcaaact cacacgcctg
    52981 accgataaca atcgggcccg cacccagaat caggatactt tttatatctg tacgttttgg
    53041 catggctctt ttactcctga ttacttagcg gttttacggt actgctcaat taactcgata
    53101 aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc tggcttccgg gtgaccctgg
    53161 aagctgaacg ccggtttatc agtgcgatga atgccctgta acgtaccgtc gaacagggat
    53221 ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat ccaccgcaaa accgtggttc
    53281 tgggcggtga tcatcaccac gtttttctcc acatctttaa ccggatggtt gccgccgtgg
    53341 tgaccaaatt tcattttgac agtcttcgca ccgctcgcca gcgccagcag ctgatgaccg
    53401 aggcagatgc cgaatatcgg aatatcggtt tcgaggaatt tctggatggc ggtaatggcg
    53461 taatcgcacg gggccgggtc gccaggaccg ttggagagga agatgccgtc tggattcatt
    53521 ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg tcaggcgaca gcctctatcc
    53581 accagcatcc gcaggatgtt gcgcttggca ccaaaatcat aagccacgac gtggaacggc
    53641 agctcgtctt cttttttcgc ttctggcagg ccgccggtca acgtccagct cccttgtgtc
    53701 cagctatagg cttctgcggt ggtcacttct tttgccagat ccatgccatt cagacctggg
    53761 aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt tatcgcctgc gataatgcag
    53821 ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct tacgggtatc gatatcggca
    53881 atcgccacga tgttatggcg cttcaggtaa gaagagaggt cttcggtatt acggaagttg
    53941 ctggcaatca gcggcagatc gcgaatcacc agaccttgtg catgtacctg agaagattct
    54001 tcatcggcgt cattagtgcc gacattgcca atatggggat aagtaagagt aacgatttga
    54061 cgagaatagg aaggatcagt gaggatttct tgataaccgg tcattgaagt attgaaaacg
    54121 acttccccaa ccgccgaacc tgttgcccct atggcccgac cgtgaaactg ggttccgtct
    54181 tccagaacca atagcgctga cttaatcaaa acaccctcca gagaatattc actcacttta
    54241 tttgcatatt aattcacaat gatgacgtaa atcaatgcaa atctgcttac cgacgaattt
    54301 ctggcaaacg gcggcattct ggagatatgg gcgctaaaag tcaacttaat ggcgggattt
    54361 tttatattat tttgtggctc atgcatgttt tacaggcatt aagcgacaaa aagatcatat
    54421 aacctgttct ggaaaacgct tgcgcagcaa gaagattgca tgaaataaaa gcgagcaggg
    54481 aaaagtggac caaatggtca aaatttacat tgaaacaaca aaaaccgaag acaaaacatg
    54541 taatttttgg cacttataga caatttaaaa acataacact aaaaataaaa gggccaaaaa
    54601 ttaaagccct ttataatcaa taaattaaat gtgttattct tgcaccataa caaatatttt
    54661 gtggttacaa actattgaga tcaagcacat ctcgcatatc aaaaagacca ctttccttac
    54721 cactcaacca caaagccgat cttaccgcgc cgttagcaaa tgtcatgcgg ctggacgcct
    54781 tatgggtgat ctccagacgc tcgccaatat cggcaaacat cgcggtatgt tcgccaacga
    54841 tgtcacctgc acgcacggtg gcaaaaccaa tggtgccagg cacacgttca ccggtgtggc
    54901 cttcacgact gtagaccgcg caatctttca gatctttatc aagggcgtgg gcgatcgcct
    54961 ctcccattgc cagtgcggtg cctgacggcg catcaacttt atgtctatga tgtgcttcaa
    55021 taatttcgat atcggtgtag tcacccatca ctttggctgc tttctccagc agcttaagca
    55081 tgacgttaac gccaacgcta aaattggcgg caaagacaat cgcaatatcg gcggcggcgt
    55141 cacgaatcgc ctgtttaccg gcttcgtcaa accccgtagt gccgatcacc atccctttgc
    55201 catgctggcg acaaaaagcg agatggttca gcgtaccttc cggacgggta aaatcgataa
    55261 acacatcaaa atcatctttt accgcatcga ggctgctttg cacggtaacg cctgttttcc
    55321 ctgcaccagc cagctcaccg gcgtcgctgc ccagtaaaga agatccttcg cgctccagcg
    55381 ccgcgcccag ctgcacgcct tctaatgcca gcgccgcctg aatcaactgg cggcccatac
    55441 gccccccggc tcccgcgatg gcaacgcgga tgtttgcatc atgcatagct attctctttt
    55501 gttaatttgc atagaccgtt ttcagagtaa ccagcgcatc gctacgccgc cagccaaaaa
    55561 cgctgataat tagaaattaa tgataaacag ggagggatat cagtgaaagg catgagtcga
    55621 tgaatgactg catgccgata acatgtgaca ggttacgacg ccagagccag cacctcagcc
    55681 acccactgct ggaagccttt cacatccaga tccaatgcca cctttacatt ggctggtttg
    55741 cccaggcaac cgtcgatatc aaccaccgtc gtgcctgagg taaattcgcc ttgagtttct
    55801 accgcgacaa aacacggctt gagggtgaat aagtccgggc gcaccagcca ggcgatggcg
    55861 cagagatcgt gcattcgcag accgctttgc atactgccgc tacggtagtg gctaaacagt
    55921 gcatgaagca tttttccggt gcgattcaac tccggcaaag tagcgagata gtcaggggtt
    55981 aatattgcct gattggtgac atccagaccg cacatgacga tttcgattcc gctacggaag
    56041 acacaggcag ccgcttctgg atcggcagcg atattaaact cggcgtttgg cgtacagttg
    56101 ccgcgtccgg cagaaccacc catgatcacc agacggcgaa tatacggctt gcattccggg
    56161 cattgtgaaa gtaacagcgc aatattggtt aacgggccga tggccaccag ggtaacaggc
    56221 tctggtgcac gcatcagggc atcccgaatc gccagaaacg ccggtatccc gagcggcttt
    56281 cggttgtgct caacaaagtc gtagccagcc attcccgatt cgccgtgcac agatgccgca
    56341 tcacgcggtg cgcgtaccag cggcacagcg gccccttggg cgagcggaat ctccgcattc
    56401 cagaaatgca gcagttgcag ggcattgcgg gtagttttct caaccgagac attacccgcg
    56461 acggtggtca tcagttgcag gtcgagttcg ggtgcaaaaa tcgcggcggc aatggcgacg
    56521 gcatcgtcaa tgccggggtc agtatcgagg aagataggta aacgcatgtt ttctccataa
    56581 aaaatgccgg taacaagacc ggcattttcg cataacttag gctgctaatg acttaatcga
    56641 cttcacgaat atcgacacgc agctctttcg gcacttcgaa aacaatgttt tcttcacggc
    56701 cttccagcgg aatggcttca ccaccgccca gctgctgcaa acgtgccacc acattctgca
    56761 ccagaatatc cggagccgat gcgcccgcag tcacgccgac gcatttaacc tctttcaccc
    56821 actcttcctg gatgtctttc gcatcgtcaa tcaaaaacgc gcgtttgccc atacgctggg
    56881 ccagctccgc cagacggttg gagttggagg agtttttcga accgaccacc aacacaactt
    56941 ccgcctgttc tgccagggcg cgtaccgctt cctgacggtt agtcgtggcg tagcagatgt
    57001 catctttgcg cggaccgaca attttcggga agcgtttacg cagcgcgtcg atcacatcag
    57061 acgtgtcatc caccgacagc gtggtctggg tcataaagga gagcttctct tcgtttttga
    57121 ccgtcagttt ccacacatcg tccggcgatt cgaccagata cattccccct tccgggttac
    57181 tgtactggcc cattgtccct tccacttccg ggtgcccggc gtgaccgatg agaatagatt
    57241 cttcgccacg gcgactggcg cgggcgactt ccatatgcac tttggtcacc agcggacagg
    57301 tcgcgtcaaa taccgtcaaa tcgcgacttt ttgcttcgtt acgtaccgcc tgagaaacac
    57361 cgtgtgcgga gaaaatcagg atcgcgccgt ccggtacttc gctaatctgc tcaataaaga
    57421 tagccccacg ctcacgcaag ctatcgacca catagcggtt atgtaccact tcgtgacgga
    57481 catatatcgg tgcgccgtaa atggccagcg cgttttcaac aatgctgata gcgcggtcta
    57541 ccccggcaca aaaaccacgc gggttggcca acaggatctg catgttacgc ctccagtgcc
    57601 ggatcgattt ccagcacttc aatatcaaaa tgaacggtct gcccggccag cggatggttg
    57661 aaatcaacgg taatggagtc gccgttaatt tcgcggatca cgccaggcat ctcactgcca
    57721 tccattgcgg taaaaagcat gattgcgcca atttctggct cgcctgcatc cataaattca
    57781 cggcgggaga agtactgaat caggtccggt gacggcacgc caaacgccgc atctggctcc
    57841 aacgagaagg tggttttatc gcccactttc agccccaaca ggtgttgctc cagcccttca
    57901 gaaagagaag catcacccag gcggaacagc gccggtttac cgttgttgcg ggtagactcg
    57961 gcggtggtgc catcgtcgag ttttagcgtg aagtgcacca ggacggcgct attgctctgt
    58021 acagattcag acatgcaggt ttgctcttat atttaaggat ttttgccgga tgcggcgtaa
    58081 acgccttatc cggcctacga tttcgcagca atctgtaggc cggataagat gcgtcagcat
    58141 cgcatccggc agggtttatt attgtttttt cgctttagaa ggtaaaaaac cttccagcac
    58201 aatcagtgcc gcaccgacac aaatggcagt atcggcaagg ttgaaggtgg cgaagtgcca
    58261 gtcgccgacg tagaagtcga tcatatcgac aacgaagccg tgccacaggc ggtcgaacag
    58321 gttgcccagc gcgccgccaa taatcagcgc gtaagcgata ttgtttagct tctgcgtggc
    58381 cttcgagcga tacatcatca ctgccaggat cacgctaata ccaatcgcaa taccggcaaa
    58441 gaaccaacgc tgccaaccgc cgctatcggc aaggaaacta aacgccgcgc catagttacg
    58501 cgcataatgc agattaagcg acgggaacag cgggaccgta tcccccagag caaagttctg
    58561 gaggatcagg tatttgctgc ccagatcgat aatcagcacg actaccacca gccacagcca
    58621 gcgtagccct gttgaacaga tcgattgact catcaggcaa acttacgttt ttcaccgtca
    58681 ccggcgacgt tgctgacaca gcggccgcag atttctgcgt gttccgccac cttgccgaca
    58741 tcctgggtgt agtgccagca gcgtgggcac ttctcacctt cggctttact caacgcgact
    58801 ttcagccctt tgagtacttc gctctgctga gcatcagcag gtgcgtcgtt atagtctgca
    58861 acggtagcgc cggaggtcaa caggacaaat cgtaattcat cgcccagcgc ggtcagtttc
    58921 gccgacagtt ccggttctgc atacaaggtt actgccgctt ccagcgagcc acccactttc
    58981 ttgtcggcac gcgcttgctc aatgactttg ttcacttcgc cacgcacttt caacagctcg
    59041 tcccagaacg catcgttcat cgcttcactg tctgccaggc caaacaggcc ttcgtaccac
    59101 tcaccggtga agacgtattt ttcacgttcg cccggcaggt agccccacac ttcatcagcg
    59161 gtgaaggaga ggattggtgc catccagcgc accagcgctt ctgcgatgtg atacagcgca
    59221 gtctggcagc tacgacgcgc cacactgtcc gctttggcgg tgtactgacg gtctttgatg
    59281 atgtcgaggt agaaggaacc catctcaacg gagcagaagc gcatcagacg ctgtaccact
    59341 tcgtggaaat cgtatgcttc gtacgccttg aggatgtctt cctgtgccgc tttcgcacaa
    59401 cctacggccc agcgatccag taccaccatc tcttccggtt tcaccatatc ttttgctgga
    59461 tcaaaaccgt tcaggtttgc cagcaggaag cgcgcggtgt tacggatacg acgatagcta
    59521 tcggcagcac gtttcaggat ctcgtcagaa acggccattt caccggtgta gtcggttgat
    59581 gccacccaca gacgcagaat atccgcgccc agtttgttca tcacatcctg cggcgaaacg
    59641 gtattgccga tggatttaga catcttgcga ccctgaccat ccacggtaaa gccgtgggtc
    59701 agtacctgac gatacggcgc tttgcctttc atcgcggtgg agatcattag ggaagacatg
    59761 aaccaaccac ggtgctggtc agaaccttcc agatacatgt ccgctgcgtg accggcaaat
    59821 tccggacgca cgtcaacaac agaagagtgg gtagatccgg agtcaaacca tacatccaat
    59881 gtgtccggca ctttcacgta ctgatcagct tcatcgccga ggatctcttt cgcatcaaga
    59941 tcccaccacg cctggatgcc gtcaacttca acgcgttttg ccacttcttc cattagttcg
    60001 agagtacgcg gatgcagctc ttccgtgtct ttgtgcacga acagtgacat cggtacgccc
    60061 caggtgcgct gacgggagat acaccagtca ggacggttag caaccatcaa ctcgatacgc
    60121 gcctggcccc agtccgggat ccactgcacg cctttgatct ctttcagtga ctgcgcacgc
    60181 agacctttct gatccatgct gacgaaccac tgcggcgtcg cgcggaagat gatcggcgtt
    60241 ttgtgacgcc agcagcacgg atagctgtgc tgcattttct caacgtgcag cagcgcgcct
    60301 ttttcctgca gcagcgcaac gacgatgtcg ttcgctttga agacgttcac gccatccagc
    60361 gtcggataag tgcccggcag ataagtgccg tccgggccaa ccgggttagc ggtttccagg
    60421 ccgtatttct gaccgatcac atagtcgtcc gggccgtggc caggcgcggt gtgaacggca
    60481 ccggtaccgg catccagggt aacgtgatcg ccgaggattg ccggaacgtc gaagcccata
    60541 aacggatggg taaagcgcag caactcaagc tccgcacctt ttaccgtgcc gagaatggtg
    60601 taatcggtca cgccgatacg ctgcattacg ctttcaacca gatctttcgc cagaatcacg
    60661 gcctgaccgt cgatctgcac cagcgcatag tcgaaatcag gtgcaataga gattgcgcgg
    60721 ttcgcaggca gagtccacgg cgtagtggtc cagatcacca gcgagattgg gccgttaacg
    60781 ttgcttacgg caaattttgc tttcagtgca tcctgatcga ccgcctgaaa agcaacgtcg
    60841 atggacggag aagttttgtc gtaatactca acttccgctt ccgccagcgc agaacggcag
    60901 tcaacgcacc agtgaactgg cttcgcgcct ttgtgcaggt gaccgttgcc gatgattttg
    60961 cccagcgcgc ggatgatgtt ggcttcagtt ttgaagtcca tggtcaggta cgggtgcgac
    61021 cagtcgccca gcacgcccag acggataaag tctttgcgtt gaccgtcaac ctgggtcgcc
    61081 gcgtattcgc ggcacttggc gcggaactcg gcggcggtga atttctcacc cggcttaccg
    61141 tattcttgct ctactttcag ctcgatcggc agaccgtggc agtcccagcc aggcacatac
    61201 ggcgagtcat aaccggaaag ccctttggac ttcacgataa tgtctttcag aatcttgtta
    61261 accgagtgac caatatgaat gctgccattc gcataaggag ggccatcatg cagaatgaag
    61321 gtttttttgc cttttttagc cgcacggatg atgccgtaca gatcatcatc agtccaacgc
    61381 gccagcattc ccggttcgcg cttggcgaga tcgccacgca tcgggaaccc tgtttccggc
    61441 aaattcaggg ttgatttata gtcactcatc agattctcgg ttccgtattt cggtttgatt
    61501 acataacagg cttaagccgg ttttgttagc ccaaaaaatt cgcgggcggt taattcatca
    61561 cgcgcaatct gcgctttcag ttcgtccagc gacgcaaatc gctgctcatt gcgtattttt
    61621 ttacgcagca ctacttgtat atggcgaccg taaaggtcca ttgcaacatc taacaaatgc
    61681 acttccagct gctggcgaat accggcaacc gttgggcgtg ttccgatgtt tgccacgccg
    61741 ggtaacggct tttcaccgag gcccagcact tctaccgcat aaaccccttt caccggggaa
    61801 acctgacggc gcagcggtac attcgccgtc gggaaaccta tagtgcgccc taattcatca
    61861 ccgtggacta cacgcccgga gatggcaaac gggtgcccca gtaaactttc tgccagagcc
    61921 agattgtcat cagcaagggc ctgacgcacg gcggtgctgc tgatacgcac gccaccttcg
    61981 caaaaagttt gcgtactggt gatatcgaag ccgtattcca tgccagcttt ctgtaataac
    62041 aagaaatcgc cttcacgacc agcgccaaag cggaaatcat cacctacggc aagaaatttt
    62101 acgcgcaaat gcttcaccag aagatcgctg atgaaatttt gcgcggttaa cgccgcgaaa
    62161 cgcctgtcga aacgtacgca cagcacgtaa tcaacgccac actctgcaag gtaacgcaat
    62221 ttttcccgca gccgggtcag tcttgccggg gctttatcgg tagcaaacag ttccagtggt
    62281 tgaggttcaa aaagcatcac catcaccggt aagttgcgct tgcgcccttc ttcctgcaag
    62341 ccctgtaaca gcgcgcgatg accgcgatgc acgccgtcga aattaccaat agtcagcaca
    62401 cacccttctt gcggggcctg gctgagatta tgtatgccgc gtatcagctt catgtctggc
    62461 tcaaaacagt gaaaatcgtc cgagtatacc ttgtacagcg gtcaaggtta accggcgatt
    62521 gagtaccgaa atcagaaaga agtggctttt cattgccatg gcgcaaatca cgggaagaaa
    62581 gtgaccgcct gctgcaattt ttatcgcgga aaagctgtat tcacaccccg caagctggta
    62641 gaatcctgcg ccatcactac gtaacgagtg ccggcacatt aacggcgctt atttgcacaa
    62701 atccattgac aaaagaaggc taaaagggca tattcctcgg cctttgaatt gtccatatag
    62761 aacacatttg ggagttggac cttggctaat atcaaatcag ctaagaagcg cgccattcag
    62821 tctgaaaagg ctcgtaagca caacgcaagc cgtcgctcta tgatgcgtac tttcatcaag
    62881 aaagtatacg cagctatcga agctggcgac aaagctgctg cacagaaagc atttaacgaa
    62941 atgcaaccga tcgtggaccg tcaggctgct aaaggtctga tccacaaaaa caaagctgca
    63001 cgtcataagg ctaacctgac tgcacagatc aacaaactgg cttaatcgcc aatttgctga
    63061 agctttgtga aaaagcccgc gcaagcgggt ttttttatgc ctgctgcttt tatgatgcgt
    63121 gagagaacac actaccggtt gccagatagt caaaagatgc tggcttttgc agacatgccg
    63181 ttacactttc tgaactcttc gtggcttaag aatcgtagaa ccatcggctg atacattctg
    63241 aacaagaata tctactcaat ctccagactc ccctacaaag aatgtggata ttgtgataca
    63301 cattgaggta ttaaacttaa gaccactcta tttttgaatt aaattcctat acactttaac
    63361 cattcataaa tatctcctta ttagaacttg taaataatta acaacaatca acacaggtt
//
LOCUS       DAZKIV010000009        61340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZKIV010000009 DAZKIV010000000
VERSION     DAZKIV010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.009"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..358
                     /locus_tag="ACHOT6_000610"
     CDS             <1..358
                     /locus_tag="ACHOT6_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP9945886.1"
                     /translation="TGYQPLILQSIKTKLLSSRLNKFNDFEDSFNGLGISVHDISAQK
                     ISLLSFQKYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFFRIWFFLQRHKDFAFK
                     PFFTNFNTIERIENYL"
     gene            355..834
                     /locus_tag="ACHOT6_000611"
     CDS             355..834
                     /locus_tag="ACHOT6_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001688100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFP9945887.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDRDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(888..1838)
                     /gene="corA"
                     /locus_tag="ACHOT6_000612"
     CDS             complement(888..1838)
                     /gene="corA"
                     /locus_tag="ACHOT6_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFP9945888.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1990..2046)
                     /gene="ysgD"
                     /locus_tag="ACHOT6_000613"
     CDS             complement(1990..2046)
                     /gene="ysgD"
                     /locus_tag="ACHOT6_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFP9945889.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2208..2972
                     /gene="yigE"
                     /locus_tag="ACHOT6_000614"
     CDS             2208..2972
                     /gene="yigE"
                     /locus_tag="ACHOT6_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFP9945890.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGL
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3119..5281)
                     /gene="uvrD"
                     /locus_tag="ACHOT6_000615"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(3119..5281)
                     /gene="uvrD"
                     /locus_tag="ACHOT6_000615"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFP9945891.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(5365..6081)
                     /gene="yigB"
                     /locus_tag="ACHOT6_000616"
     CDS             complement(5365..6081)
                     /gene="yigB"
                     /locus_tag="ACHOT6_000616"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFP9945892.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(6081..6977)
                     /gene="xerC"
                     /locus_tag="ACHOT6_000617"
     CDS             complement(6081..6977)
                     /gene="xerC"
                     /locus_tag="ACHOT6_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFP9945893.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(6974..7681)
                     /gene="yigA"
                     /locus_tag="ACHOT6_000618"
     CDS             complement(6974..7681)
                     /gene="yigA"
                     /locus_tag="ACHOT6_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFP9945894.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(7678..8502)
                     /gene="dapF"
                     /locus_tag="ACHOT6_000619"
     CDS             complement(7678..8502)
                     /gene="dapF"
                     /locus_tag="ACHOT6_000619"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFP9945895.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(8539..8742)
                     /gene="yifL"
                     /locus_tag="ACHOT6_000620"
     CDS             complement(8539..8742)
                     /gene="yifL"
                     /locus_tag="ACHOT6_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9945896.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            9093..9647
                     /locus_tag="ACHOT6_000621"
     CDS             9093..9647
                     /locus_tag="ACHOT6_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFP9945897.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSITCCGDVSGDTAEVIWT
                     LDMTHEQYLKGMRLEKRNKTLPLPKREWGENYLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            10019..11461
                     /locus_tag="ACHOT6_000622"
     CDS             10019..11461
                     /locus_tag="ACHOT6_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9945898.1"
                     /translation="MHFRNVVCLLSCSLFLSPAWGCKLSSVDPNEYQQQGKGVVYLRP
                     NQNTNLSLPNVNYKRLRRLANLLIDPATLEDWEEIPPLTDLSTDYDVRGANAWYPHYS
                     WHSDGRYIIYAGKIVQNPAGTPPVDVTSFKAWGDFAADKNSLYFRGKRTDDNGGDKPL
                     DSKTLHQRNLPPPWEPDVLGLILRDANFLYINGHRIAETNSFRVLAQKSWDKPGKFSM
                     KFSCIRKPFGPWDTLARTQTKIMLNGEQLDADPDTFTVVRWIPEALISWRDKNGFHRK
                     VLDQYALDSNEEYSCGSFNVKEHRVTWRSSFPGKGAECQQEEIPGLDPEQFHPISDAV
                     AQYQDKLYVIETTPFDELKLNIVTLDDPKLIINKRFNAGKRHGYLLTRKGDEFGDSGL
                     QVFESAGPLILFDNHIPSEREAHQGSSNPYIQKWLARDDKYVYRFDGMQLWRYHTADP
                     RAVRVVNDQLDGKINGDGEFIPTPRDEAKK"
     gene            11458..12768
                     /locus_tag="ACHOT6_000623"
     CDS             11458..12768
                     /locus_tag="ACHOT6_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003879755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY domain-containing protein"
                     /protein_id="HFP9945899.1"
                     /translation="MKVIKKSISLALILLGYTVAIPAVQAEGYEYYSNQGALVYGSNF
                     GGQKKIIAKFTGINSRDLYPVENISGEQERYIIEDEVTTIKEEYHWITDGHVILWRGK
                     IVSNPPGTPTVDVASFKALGRFAVDKYSLYFDGQRTESNSGASKVDLATLKAIEDNSS
                     TLVDRHNLYLLGRRQASSDDFSVLQSKLWGDIERFRPRNEGATSRDLLIRHRQDVFLN
                     GQRIADVDADTFHIIRWIPNSLLVYRDKHGEHRYAYGNPAGKMATTDDANSFEIGEKT
                     VRWRRQLARDDVWHTQLSWDVIWGAWQDIPNVDPEQFHLITDRIAQYQDRLYIIKLSP
                     VGEDQLNVITLDTPDLVVDHVFNGGKKHIYIIKDRTWVQDVHVIATHGPLTMAEGFAW
                     DDRYVYTWWGQRPSRTESPCPEQTVEQDDGIVIKTEASECHRSP"
     gene            12799..14307
                     /locus_tag="ACHOT6_000624"
     CDS             12799..14307
                     /locus_tag="ACHOT6_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9945900.1"
                     /translation="MINTSRQDGFMHYRNVVCLLACSLFLSSAWGCRLSAPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDEWDKEPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADLESFRVLAQK
                     SWDQRGKFSTTFNPCIAVPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLAWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            14304..15617
                     /locus_tag="ACHOT6_000625"
     CDS             14304..15617
                     /locus_tag="ACHOT6_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFP9945901.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRADLRLVKNISGKTGYIPSDEISIKNEEYHWVTDGRVILWRGKIVS
                     NPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTSDRQWSKWIDLPGIEPEQFHLITGNIAQYKDRLYVTKLSIFGEDQLEIIPLDTP
                     DLVIDRSFNGGKQHAYFIRQLRSKSLQIIPVNGPLTKNDRFAYDDRNVYTWTDTEVRI
                     TPSPCPAKTRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
     gene            15665..15985
                     /gene="cyaY"
                     /locus_tag="ACHOT6_000626"
     CDS             15665..15985
                     /gene="cyaY"
                     /locus_tag="ACHOT6_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFP9945902.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(16025..18571)
                     /gene="cyaA"
                     /locus_tag="ACHOT6_000627"
     CDS             complement(16025..18571)
                     /gene="cyaA"
                     /locus_tag="ACHOT6_000627"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFP9945903.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            18958..19899
                     /gene="hemC"
                     /locus_tag="ACHOT6_000628"
     CDS             18958..19899
                     /gene="hemC"
                     /locus_tag="ACHOT6_000628"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFP9945904.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPT"
     gene            19896..20636
                     /gene="hemD"
                     /locus_tag="ACHOT6_000629"
     CDS             19896..20636
                     /gene="hemD"
                     /locus_tag="ACHOT6_000629"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFP9945905.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            20658..21857
                     /gene="hemX"
                     /locus_tag="ACHOT6_000630"
     CDS             20658..21857
                     /gene="hemX"
                     /locus_tag="ACHOT6_000630"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFP9945906.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKMMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPQGDTP
                     AAAPQGE"
     gene            21860..23056
                     /gene="hemY"
                     /locus_tag="ACHOT6_000631"
     CDS             21860..23056
                     /gene="hemY"
                     /locus_tag="ACHOT6_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFP9945907.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            23735..23872
                     /locus_tag="ACHOT6_000632"
                     /pseudo
     CDS             23735..23872
                     /locus_tag="ACHOT6_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(23980..25215)
                     /gene="aslB"
                     /locus_tag="ACHOT6_000633"
     CDS             complement(23980..25215)
                     /gene="aslB"
                     /locus_tag="ACHOT6_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9945908.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRSASDIMQAHLLVVNK"
     gene            complement(25362..25438)
                     /locus_tag="ACHOT6_000634"
     tRNA            complement(25362..25438)
                     /locus_tag="ACHOT6_000634"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25402..25404),aa:Pro,seq:tgg)
     gene            complement(25481..25567)
                     /locus_tag="ACHOT6_000635"
     tRNA            complement(25481..25567)
                     /locus_tag="ACHOT6_000635"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25531..25533),aa:Leu,seq:cag)
     gene            complement(25588..25663)
                     /locus_tag="ACHOT6_000636"
     tRNA            complement(25588..25663)
                     /locus_tag="ACHOT6_000636"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25628..25630),aa:His,seq:gtg)
     gene            complement(25722..25798)
                     /locus_tag="ACHOT6_000637"
     tRNA            complement(25722..25798)
                     /locus_tag="ACHOT6_000637"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(25762..25764),aa:Arg,seq:ccg)
     gene            complement(25901..27286)
                     /gene="yifK"
                     /locus_tag="ACHOT6_000638"
     CDS             complement(25901..27286)
                     /gene="yifK"
                     /locus_tag="ACHOT6_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9945909.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(27477..28217)
                     /gene="wecG"
                     /locus_tag="ACHOT6_000639"
     CDS             complement(27477..28217)
                     /gene="wecG"
                     /locus_tag="ACHOT6_000639"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFP9945910.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(28220..29572)
                     /gene="wzyE"
                     /locus_tag="ACHOT6_000640"
     CDS             complement(28220..29572)
                     /gene="wzyE"
                     /locus_tag="ACHOT6_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFP9945911.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(29569..30648)
                     /gene="rffT"
                     /locus_tag="ACHOT6_000641"
                     /gene_synonym="wecF"
     CDS             complement(29569..30648)
                     /gene="rffT"
                     /locus_tag="ACHOT6_000641"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFP9945912.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVWGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(30645..31895)
                     /gene="wzxE"
                     /locus_tag="ACHOT6_000642"
     CDS             complement(30645..31895)
                     /gene="wzxE"
                     /locus_tag="ACHOT6_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFP9945913.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(31897..33027)
                     /gene="rffA"
                     /locus_tag="ACHOT6_000643"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(31897..33027)
                     /gene="rffA"
                     /locus_tag="ACHOT6_000643"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFP9945914.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(33032..33706)
                     /gene="rffC"
                     /locus_tag="ACHOT6_000644"
                     /gene_synonym="wecD"
     CDS             complement(33032..33706)
                     /gene="rffC"
                     /locus_tag="ACHOT6_000644"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFP9945915.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(33684..34565)
                     /gene="rfbA"
                     /locus_tag="ACHOT6_000645"
     CDS             complement(33684..34565)
                     /gene="rfbA"
                     /locus_tag="ACHOT6_000645"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFP9945916.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(34584..35651)
                     /gene="rffG"
                     /locus_tag="ACHOT6_000646"
     CDS             complement(34584..35651)
                     /gene="rffG"
                     /locus_tag="ACHOT6_000646"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFP9945917.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(35651..36913)
                     /gene="wecC"
                     /locus_tag="ACHOT6_000647"
     CDS             complement(35651..36913)
                     /gene="wecC"
                     /locus_tag="ACHOT6_000647"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFP9945918.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(36910..38040)
                     /gene="wecB"
                     /locus_tag="ACHOT6_000648"
     CDS             complement(36910..38040)
                     /gene="wecB"
                     /locus_tag="ACHOT6_000648"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFP9945919.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(38096..39142)
                     /gene="wzzE"
                     /locus_tag="ACHOT6_000649"
     CDS             complement(38096..39142)
                     /gene="wzzE"
                     /locus_tag="ACHOT6_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFP9945920.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(39154..40257)
                     /gene="wecA"
                     /locus_tag="ACHOT6_000650"
     CDS             complement(39154..40257)
                     /gene="wecA"
                     /locus_tag="ACHOT6_000650"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFP9945921.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(40497..41756)
                     /gene="rho"
                     /locus_tag="ACHOT6_000651"
     CDS             complement(40497..41756)
                     /gene="rho"
                     /locus_tag="ACHOT6_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFP9945922.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(41841..41942)
                     /gene="rhoL"
                     /locus_tag="ACHOT6_000652"
     CDS             complement(41841..41942)
                     /gene="rhoL"
                     /locus_tag="ACHOT6_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFP9945923.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(42083..42412)
                     /gene="trxA"
                     /locus_tag="ACHOT6_000653"
     CDS             complement(42083..42412)
                     /gene="trxA"
                     /locus_tag="ACHOT6_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFP9945924.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            42543..43808
                     /gene="rhlB"
                     /locus_tag="ACHOT6_000654"
     CDS             42543..43808
                     /gene="rhlB"
                     /locus_tag="ACHOT6_000654"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFP9945925.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            43942..45426
                     /gene="gppA"
                     /locus_tag="ACHOT6_000655"
     CDS             43942..45426
                     /gene="gppA"
                     /locus_tag="ACHOT6_000655"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFP9945926.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(45473..47494)
                     /gene="rep"
                     /locus_tag="ACHOT6_000656"
     CDS             complement(45473..47494)
                     /gene="rep"
                     /locus_tag="ACHOT6_000656"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFP9945927.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            47711..48160
                     /locus_tag="ACHOT6_000657"
     CDS             47711..48160
                     /locus_tag="ACHOT6_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFP9945928.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQFSTKYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            48359..48640
                     /gene="ppiC"
                     /locus_tag="ACHOT6_000658"
     CDS             48359..48640
                     /gene="ppiC"
                     /locus_tag="ACHOT6_000658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFP9945929.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGKFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(48727..50202)
                     /gene="ilvC"
                     /locus_tag="ACHOT6_000659"
     CDS             complement(48727..50202)
                     /gene="ilvC"
                     /locus_tag="ACHOT6_000659"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFP9945930.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            50352..51245
                     /gene="ilvY"
                     /locus_tag="ACHOT6_000660"
     CDS             50352..51245
                     /gene="ilvY"
                     /locus_tag="ACHOT6_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFP9945931.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(51297..52841)
                     /gene="ilvA"
                     /locus_tag="ACHOT6_000661"
     CDS             complement(51297..52841)
                     /gene="ilvA"
                     /locus_tag="ACHOT6_000661"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFP9945932.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(52844..54694)
                     /gene="ilvD"
                     /locus_tag="ACHOT6_000662"
     CDS             complement(52844..54694)
                     /gene="ilvD"
                     /locus_tag="ACHOT6_000662"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFP9945933.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(54759..55688)
                     /gene="ilvE"
                     /locus_tag="ACHOT6_000663"
     CDS             complement(54759..55688)
                     /gene="ilvE"
                     /locus_tag="ACHOT6_000663"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFP9945934.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(55708..55971)
                     /gene="ilvM"
                     /locus_tag="ACHOT6_000664"
     CDS             complement(55708..55971)
                     /gene="ilvM"
                     /locus_tag="ACHOT6_000664"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFP9945935.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(55968..57614)
                     /gene="ilvG"
                     /locus_tag="ACHOT6_000665"
     CDS             complement(55968..57614)
                     /gene="ilvG"
                     /locus_tag="ACHOT6_000665"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFP9945936.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(57617..57667)
                     /gene="ilvX"
                     /locus_tag="ACHOT6_000666"
     CDS             complement(57617..57667)
                     /gene="ilvX"
                     /locus_tag="ACHOT6_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFP9945937.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(57754..57852)
                     /gene="ilvL"
                     /locus_tag="ACHOT6_000667"
     CDS             complement(57754..57852)
                     /gene="ilvL"
                     /locus_tag="ACHOT6_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFP9945938.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            58205..59725
                     /gene="yifB"
                     /locus_tag="ACHOT6_000668"
     CDS             58205..59725
                     /gene="yifB"
                     /locus_tag="ACHOT6_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFP9945939.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(59750..60088)
                     /gene="maoP"
                     /locus_tag="ACHOT6_000669"
     CDS             complement(59750..60088)
                     /gene="maoP"
                     /locus_tag="ACHOT6_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFP9945940.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            60207..61046
                     /gene="hdfR"
                     /locus_tag="ACHOT6_000670"
     CDS             60207..61046
                     /gene="hdfR"
                     /locus_tag="ACHOT6_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFP9945941.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDILKINVLD
                     EVY"
     gene            complement(61141..61216)
                     /locus_tag="ACHOT6_000671"
     tRNA            complement(61141..61216)
                     /locus_tag="ACHOT6_000671"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61181..61183),aa:Trp,seq:cca)
     gene            complement(61225..61301)
                     /locus_tag="ACHOT6_000672"
     tRNA            complement(61225..61301)
                     /locus_tag="ACHOT6_000672"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(61265..61267),aa:Asp,seq:gtc)
BASE COUNT        15187 a        16307 c        15528 g        14318 t
ORIGIN      
        1 gacaggatac caacctctta tcctgcaaag cattaaaacc aaattacttt catcaagact
       61 aaataaattt aatgacttcg aggacagttt taatggctta ggaatcagtg ttcatgatat
      121 ttcagctcaa aaaatatctc ttttaagttt tcaaaaatac gctataggat ggagtgcaac
      181 aattcatttt gtagcacagg accacttcgg tcttgatgta actgatatca aaaacaagac
      241 ttacagcaaa tatagatttt ttcgcatatg gttcttttta caacggcata aagactttgc
      301 tttcaaacct tttttcacca attttaacac tatcgaaaga atcgaaaatt atctatgaaa
      361 agcaaactcc agaaaattat attatgttta tttcttctat gctgcatcta taatttatgg
      421 acattgcgtc ctgtacagat tttgtataca tactctgatg ctggcaattc agtctttctg
      481 gtcgttgatc atctgccatg gactgaccgc gataaaataa attggtatct gaagcatcag
      541 aatgaaatta aaaatcaaca tcccctacca gaaggtagtt ggcatacctg gtatgtcatt
      601 gatatcggta atggcttcac tgactataaa aaatatatcg aagggccgta tgaagatctc
      661 tactgtttcc cgacaattaa gagcaatgat aattgcatcg tgaaaaatta cttaatggtt
      721 attaatgaat acccttatcg taacactcat attggtatta acgattttac tgagtatcaa
      781 ttaacacaag aaaataaaat agagcgagtt ttcaatccgc atgactttaa ataagacaat
      841 ttttagaaat aagccggata aaaaaatagc tttatccggc actcatatta cagccagttc
      901 ttgcgcttaa agtacagata cggtgccagg cccgcgagga tcataaagat aatcgcgcct
      961 gggtagccga aactccactt cagttctggc ataaactcaa agttcatccc atagctggaa
     1021 gcaacgagcg tcggcggcag gaataccacg gataccaccg agaaaatttt gatgatgcgg
     1081 ttctgctcga tgttgataaa acccattgcc gcctgcatca ggaagttcac cttctggaac
     1141 agggattcgt tatgcggcag cagggattcg atatctcgca ggatttcacg cgcctgctcc
     1201 agttgcccac ccggtaaacg cgctttacgc accaggaagt tgagcgcgcg ctgggtatcc
     1261 atcagacaca ggcgaacttt ccagccgata tcttccagtt ccgccagcgt ggagagcgcc
     1321 tcgtcgtact catcgccctg atgcccttcc ataatcaccc ggctcaactg ctccaggtcg
     1381 ctatagatat tttcaatttc atctgccaac tgttcgattt tggtttcgaa cagatccagc
     1441 agcaactcgt aggcgttacc gtctaccatc gactggctac gggcacgcat acgatacaga
     1501 cgaaaagcgg gcagttcacg ctcacgcaga gtaaacagac gaccatcacg gatggtaaat
     1561 gccacagtgg agttaccggc gtgatcttcc gcatcttcaa agaagaagaa ggagtgaata
     1621 tgcaggccgt catcgtcttc aaagaaacgt gccgatgctt cgatgtcttc cagttccggg
     1681 cgggttgcca ggctctggcc aagttcagat tgtacgcgca gtcgctcgtc gtcgtccggt
     1741 tcgacaagat caatccatac tgcatttaca aggggttgtg actcttcgac ttccagccgg
     1801 gtcagtcggt tattttccag ttgaaatgcg ctcagcatga ccgggactcc caatgcttaa
     1861 aatatcggac agttcggtgg gcacacagaa acaaattggg tttcagacca ttaaacagcc
     1921 tgactcagcg cgacgggaaa aattgaggtc gctgacaacc gctaaggcta tcagcaaaaa
     1981 gggatagcct taggagttga tcctggatga caggatagtg agccagtatc tactgggtgt
     2041 gtccaaggcg aatgtcctct tagagtgatc gtgcgcgcat gttacgccag caaaattttg
     2101 ccgtcaacac gcaacagaac accacagaac aatagttgaa cttcgcgctt tttggtactc
     2161 gtcaaggtcg ctaacaggat aaaaaaatcc aacatccagg acctataatg gcgcatcggc
     2221 tactcattgg taaaggaatg atcaccttga atctcaaacg gatttttctc accctcacct
     2281 tacttccctt gtttgctgtt gccgctgatg attgcgcact ctcagatccg acgctgaccg
     2341 tacaggcgta taccgttaat cctcaaacag agcgggtgaa aatgtactgg caaaaagcca
     2401 atggcgaagc gtggggaacg ttacatgctc tgctggcgga tattaatagt cagggtcagg
     2461 tgcagatggc gatgaacggc ggcatctatg atgaaagcta tgcgccgctc ggtttgtaca
     2521 tcgaaaacgg tcagcagaag gtggcgttaa atctcgcttc aggtgaaggg aatttcttta
     2581 tccgtcctgg cggcgtgttt tatgtcgcgg gagataaagt cggcatcgtt cgtctggatg
     2641 ccttcaaaac cagtaaagag attcagtttg cggtgcagtc agggctaatg ttgatggaaa
     2701 acggtgtaat taatccgcgt attcatccca acgtcgcctc acgtaaaatt cgtaacggtg
     2761 ttgggattaa taaacatggg aatgccgtgt ttttgttgag ccagcaggcg acaaattttt
     2821 atgattttgc ctgttatgcc aaagcgaaac tgaacgttga gcagctgctt tatcttgatg
     2881 gcacgatttc acatatgtat atgaaaggcg gggcaatccc gtggcaacgc tatccttttg
     2941 tcaccatgat ttccgtagag cggaaaggtt aaaacgatag ttttgtgtca gatactgaag
     3001 atggcgcaga tgccggatgc ggcgtaaacg ccctatccgg cctacatgac gttgcaattt
     3061 attgaatctg aatgattttt taggccaaat aaggtgcgca gcaccgcatc cggcaacgtt
     3121 acaccgactc cagccgggcg taagctgcca ccagccattt gattccctgg ccctggaatg
     3181 ccacctgcaa acggctatgt tcaccgctgc cttccatatt gacaatggtg ccttcaccaa
     3241 acttagcgtg gcgtacgcgc tggccgagct tgtagccgct gtcgttctcg accatcggcg
     3301 tacccatccg ctgatggctg accgggcggc ttaccgtggc gcgcaggcgc acctcttcca
     3361 cacactcttc cggcagctcg ccaataaagc gcgacgggcg atggtaaacc tctttgccat
     3421 acagacggcg agtttccgcg taggtcagcg tcagtttctg catcgcgcgg gttacgccaa
     3481 cgtaggccag acggcgttct tcttccagac gcccgccttc atccagcgac atctggcttg
     3541 ggaacatgcc ctcttccata ccgacgataa acacctgcgg gaactccaga cctttcgccg
     3601 agtgcatggt catcagttgt accgcatcct gccaggtatc cgcctgccct tcgcctgctt
     3661 ccagcgccgc atgggatagg aacgcctgca gcggcattaa atcttcgtct tcttcgttgt
     3721 agctgaactg gcgcgttgcc gtcaccagtt cctctaagtt ttcgatacgc gtctgacctt
     3781 tttcaccctt ctcctgctcg tacatggtac gcaggccgga gtctttaatt acccggtcag
     3841 tctgtacatg cagcggcata tcggcagttt cctgcgctaa ggcgtcgatc agttccataa
     3901 accgctgtaa ggcgctggca gcacgtccgg cgagggcttt ttcctgcaac agttcacgac
     3961 atgcctgcca gagtgttaac tggcgatcgc gcgatgtctg acgtaccacg tccagcgtcc
     4021 ggtcaccaat accccgcgtt ggcgtattca ccacacgctc aaaggccgcg tcgtcgttac
     4081 ggttggcaat caggcgcaga tacgagagcg catctttgat ttcctggcgt tcgaagaagc
     4141 gcatcccgcc gtaaatacgg tacggcatac tcgcctgcaa cagtgcttct tccagcacac
     4201 gcgactgggc gttactgcgg tagagaatgg cgcactcggc aagcgccccg ccgttgtcct
     4261 gccaggtttt gatgcggtta accacaaaac gcgcttcatc gagttcgtta aaagcgcaat
     4321 agagggaaat aggctcaccg tccgcgccat cggtccacag ttttttaccc agacgcccgt
     4381 tattgttttc aatcagggcg ttagcggcgc tcagaatatt gctggtagag cggtagtttt
     4441 gctccagacg aatagtttcg gcaccgggga aatcattaag gaaacgctga atattctcca
     4501 cctgcgcccc gcgccagccg tagattgact ggtcgtcatc accgacgatc atcactttgc
     4561 cggtgtcgcc cgccagcagg cggatccacg cgtactgaat gttgttggta tcctggaatt
     4621 cgtccaccag gatattggta aaacgttcgc ggtagtgttg taggatatgc ggcttgttaa
     4681 gccacagttc gtgagcgcgc agcagcagct cggcgaagtc caccaggccc gcgcggtcac
     4741 acgcttcctg atacgcctga tacaccttct gccaggtctg ctccaccgga ttaccgtagc
     4801 tttgaatatg atgcggacgc aggccttcat ctttctggct gttgatgtac cacattgcct
     4861 ggcgcggcgg ccactgcttc tcgtcgaggt tcatggcttt gatcagacgc ttaagcaggc
     4921 gcagctggtc ctcgctatcg aggatctgga aatcctgcgg cagattggcg tccatatggt
     4981 gcgcacgcag cagacggtgc gccagcccgt ggaaggtgcc gacccacatg ccgccctggc
     5041 tggtgcccat cagctgcccg atacgatgac gcatctccgc cgccgctttg ttggtaaacg
     5101 tcaccgccat aatcgagtat ggcgagcagt tttccacgct catcagccag gcgatacgat
     5161 gcaccagtac gcgcgtctta ccactgcccg cgcccgccag caccagaagg ttgctgcgtg
     5221 gcgcggccac cgcttcgcgc tgtttgtcat taaggctgtc gagcaggtaa gaaacgtcca
     5281 ttggcaccgc cgcgtaaaaa taggttgggc ggagaagcgc aacgccctcc gccaaaaagc
     5341 tgggtatata tacagatttg ctgattatat cagcgaggtc agagatgcca accgggaaat
     5401 ttccagatgc ggcagtaaac ggctgtccca ggtttgcatc agatcgccat tttccggtct
     5461 gatccaacag gcctgcattc cgctgcgaat tgccccaccc acgtcagtgg tgagatcgtc
     5521 cccaacatgt aagatctcgc cgatcggcac gttgagtttt tccgcagcca aaaagtacat
     5581 atcgctgaac ggttttgagc gcccgtgcgg gccagcgcgc agcacaaact caaaataatc
     5641 ccccaaacca aacagttccg gctgggcgtt accgttggtg atcgccacca gcggccattt
     5701 cttcgccagc tgttttaagg tgtcgtgagt ttgctgcggg acgtcgattc ggctgcgcca
     5761 tttggcaaag ttgatcattg ctgcgtgtgc gcctgcactg gcttcttcgg cactcagccc
     5821 ggcgtcgagc atcgcttgtt caatcgaacg aaaacgccaa cgcgtcacgt cgtgataaat
     5881 ctcgggttcc gcttcccgta ccgcctggcg caggcgttgc agatcttcat tctggaagct
     5941 gcgcagcgcc ggatgataat tttgcacaaa ggtaagcgcc tctcgctcgg tgcgcaaaat
     6001 caccggacgg ttatcgtaaa gggtatcatc caggtcaaag gtgagcgccg agatgcgccc
     6061 caaaggccgg taaaaacgca ttatttcccc cgtttggcgc gtggatgcgc cgcatcgtac
     6121 accgaggcaa ggtgttgaaa atcaagatga gtatagattt gcgtggtgga gaggttggca
     6181 tgacccagta gctcctgcac accacgaaga tcgccgctcg actccagcat atgcgtagcg
     6241 aacgagtgac gtaatttatg tgggtgaacg tgattattca gcccttgttt tatgccccat
     6301 tcggcaaagc gtttctgcac attacgcgcg gagatacgct tgcccagttt cgacagaaaa
     6361 agcgcgtcgt cttcgctacc aaacaggtcg cgcaaatcaa gccagtgctc aatccacgcc
     6421 acagcgttgc gaccaatcgg caggcggcgc tctttgctgc ctttccccat aacccacact
     6481 tcaccagact ccaggtcgag gtgtttaata tccagcccca ccagctcaga aagacgcaga
     6541 cccgcgccgt acatcacttc cagcattgca cggtcgcgta cagcgagggg atcattgata
     6601 tcaatatcca gcagccgatt catatcgtcg acgtcgatgt ttttcggcag atgacgcggc
     6661 gctttcggtg ccgaaacacc tttcgccggg ttagctttga gttcgttctg gctgaccagc
     6721 cagtcaaaaa agctacgtag cgcagaaagc cgtaacgcca gacttgctgc tcccagccct
     6781 ttacggcgac tgcgtacagc aaaattgcgc accatcgtca catcacattg ctgccagctt
     6841 tgcaggccgt tttcgctggc aaaattgatg atcgcctcaa gctgacgctg gtagttaagc
     6901 agggttatcg ggctaagctg gcgctccacg ctcagataac gtaggtagcg ttctacatcg
     6961 gtgtgtaaat cggtcatacg cgttcaatcc aacgctccag aagctccggc aacatcagcg
     7021 caatttcatg aagtaactgc gttccttgcc cttgttgata gtgactggca tcgcgactgg
     7081 taaacagcac gacacccaaa tcagcatcgc ttcccagcat cgacatcgcc accgatccca
     7141 ccgctttcgc ttccggtagc accaccagca gctctggtcc gttaagcggc ccaagatagt
     7201 gctgttcctg ccccaaacgc tgaatacgca gcggttcgaa agactgacgg cttaatgcca
     7261 gatgagtgtg gttcgacggc gcacctaagc gccagcgatc cggaaacagg cgcagactcg
     7321 cacctgccag accgagatcg cgcgcccagc ggtgaaagcg catcagcata tcgtcgagac
     7381 tgctggcggc ggtgagactg cgctgcaggt agagtaagcg ataaaacagg ccttcgttgg
     7441 cgatagcctg ttccatcaac aacgccatgt tctcttccag aacatgaata tgattacgtg
     7501 cgcgggccat gtgccattcg accaacgaaa cggtgccgcg taccggatgc ggcacacgta
     7561 tcgcttctac tgcgcgcgca ttacggataa aaaactcagg atttttaatc agataatcga
     7621 caaccgcccg gtcatcaagc tccgtgagtg tttcctgcag ttcttcccct ggttgcttca
     7681 tagatgaata aatccgtcgt agacatgtac cgccgggcca gtcatatata acgggtgacc
     7741 cggacctttc caggcgatat caagacgacc gccggggagt tccacgcgta cttcttcggc
     7801 cagcaaacct tgctggatcc ctaccgccac cgccgcacac gcgccgctgc cgcaggcctg
     7861 ggtttctcct gccccacgct cataaacgcg taaacgaata tgctcgcgct taaccacttg
     7921 cataaaaccg atattggctc gctccggaaa acgctcgtgg ctttccagaa caggaccaag
     7981 cgtttctacc gccgcggtat cgacatcatc gacctgaatc acgcaatgcg gatttcccat
     8041 cgacaccacg ccgcataaga ttgtctgctc ggcggcgcgc ataatatagg tcttttccgc
     8101 tttgttagcg cgaaacggca cggcggaagg ttcgaagttg ggttcgccca tatttacgcg
     8161 gaccagatca tcatcggtga cggtcagaac catccgcccg ttggcggtgc tgacgcggat
     8221 atcgcgctta ttggtcagcc ctttcagacg cacaaaacgg gcaaagcagc gcgcaccatt
     8281 gccgcactgc gccacttcac tgccatcagc attgaaaatg cgatagtgaa aatccagttc
     8341 aggatcatac ggcggctcaa ccaccagcag ttggtcaaac cctactccca ggtgccgatc
     8401 agccaggcga cgaatcagct ccggtgaaaa aaagacattc tgcgttaccg cgtcgacgac
     8461 cataaaatcg ttgccaaggc catgcatttt cgagaactgc atcatttact ccaatcacgc
     8521 gggtacagaa actgactttt agtaattcac ctgggatgga ccatcgccag tggcgcgatc
     8581 gtttttatcc ggcaccgtgg attgcgtttg cgtctctacc ggtttggtcg gcggcggtgc
     8641 gtttttatct gcaggcggga aatagagcgg acctttcaga ccgcagcccg tcaggctgaa
     8701 gagagtaatt aatacagtga gtgccttaaa cacgtttttc attatggatt gcctgtaaag
     8761 ttcatcgctt tctgctttct atcatcgcag gagaaggcgt aaaagcaagc gtttagcgac
     8821 tctttatgaa gtctgaaaag tgaggaaatg ctgcgagtat aacgcttaca cgtggggatt
     8881 tatgttttgc tggtagtttc gtgatcggca cctcgcgcca ggatggcttt atgcactatc
     8941 gcaatgtggt ttgtttgttg tcttgttcat tgttcttatc gtcggcatgg ggcttttata
     9001 tagagtggcg caccatgcaa tttcgttgtc tgaccttagt cgccagtgac atacaacttt
     9061 gccaacctgg tgataagagg gaataaaatc tgatggggct atttacccaa ctggaaaaat
     9121 ttgaccaaaa gcttacgcgc ggttatgccc gctgggggcg ctgggtatgg cggacgttaa
     9181 ttgtcgttcc ggtactggtc gtgttgtgga atataggaca ggcagtatgg ggaggaccaa
     9241 gaggtggagt gattctggaa atccacagtg aaattgaccg tccgatactg ggattcagtg
     9301 ttaacggcgt ggcgggcgcg aatgcttttg caaatggtgg cggttcaatc acctgttgtg
     9361 gtgatgtcag tggggatacg gcagaggtta tctggacatt agatatgact cacgagcaat
     9421 atcttaaagg gatgcgtctg gagaaacgta acaagacttt gcctctccca aaaagagaat
     9481 ggggagaaaa ttatctgcat gtgcatttta tgcctggcga taaagtgctg ctggggtgga
     9541 gtaaagattc tttttctccg tatgaagatc ttcacaacgg ggggtacaag acccgcgttc
     9601 ggcaggatgt gaaagataaa ctttatggta cagggaaaat gaattgatag atttctataa
     9661 aaatcacgtt cggcagaaag cgtaataatg tggcgaatgt tagatacccg tggtggaacc
     9721 tgggtacaac tcgtgaaaga caataaagaa ggcaacgggg ggcggaatcc caccttgcct
     9781 cccttccagg ggtcaatccg gaatattttt atgctattag ccgtactatg gggcagttcc
     9841 gggattgtat ttatatcgtg aatgaaaata tcacatttat tccagcaact atcagaaaat
     9901 agcgatgacc tttatggatt ctgaaaagtg acgatttgct gcgagagtta cgcttacttg
     9961 tgaggattta tgttctgctg gtagtttcgt gatcggcacc tcacgccagg atggctttat
    10021 gcactttcgc aatgtggttt gtttgttgtc ttgttcgttg ttcttatcgc ctgcctgggg
    10081 atgtaagctt tcatctgtgg atcccaatga gtatcagcaa cagggaaaag gcgtggtgta
    10141 cctgcgccct aatcaaaaca ccaatctttc gctaccgaac gtgaattaca agcgcctgcg
    10201 tcggttggcg aatttgttaa ttgacccagc gacactggaa gattgggagg aaataccgcc
    10261 gttaaccgat ctgtctactg attatgatgt gaggggcgca aatgcctggt atcctcatta
    10321 ctcctggcat agcgatggtc gctatattat ttacgctggc aagatagtgc aaaatcctgc
    10381 gggtacaccg ccggttgacg tcacgtcatt caaggcttgg ggcgattttg ccgctgataa
    10441 aaatagcctc tatttcagag gcaaacgcac ggatgacaat ggtggtgaca agcctttaga
    10501 tagcaaaaca ctgcatcaga ggaatttacc gccgccatgg gaacctgacg tcctcgggct
    10561 gatattgcgt gatgctaact tcctgtatat caacgggcat cgtattgctg agaccaatag
    10621 tttccgcgtg ctggcgcaaa agtcctggga taagcccgga aaattttcca tgaagtttag
    10681 ctgcattcgc aaaccattcg gcccctggga tacgctggct cgtacacaga cgaagattat
    10741 gctcaacggc gaacaactgg atgccgaccc ggataccttc accgttgtgc gctggatacc
    10801 tgaagcatta ataagctggc gagataaaaa cggctttcac cgaaaggtac tggatcaata
    10861 tgccctggac agcaatgagg aatatagctg cggtagtttc aatgtaaagg aacaccgcgt
    10921 cacctggcgt agtagcttcc cgggtaaggg agcagaatgc caacaagaag agattcccgg
    10981 cctcgatcct gaacagtttc acccaattag cgatgctgtc gcgcagtatc aggacaagct
    11041 gtacgtcatc gaaacaacgc cattcgacga gctcaagctg aatattgtta cgcttgatga
    11101 cccgaaactg attattaata aacgcttcaa cgccgggaag cgtcacggtt acttgctcac
    11161 aagaaaggga gacgagtttg gggattctgg tttacaggtg tttgaatccg ccgggccact
    11221 cattttattt gataaccaca tccccagcga acgggaagcg catcagggta gcagcaaccc
    11281 ttatatccaa aaatggttag cccgcgacga caaatatgtt tatcgcttcg atggcatgca
    11341 gctctggcgc tatcacaccg cagatccccg agcggtgcgc gtcgtgaacg atcaactcga
    11401 tggcaaaatt aacggtgatg gcgagtttat acccactccg cgcgatgagg ctaaaaaatg
    11461 aaagtgataa aaaaaagtat ctcactggcg ctcatcctgt tggggtacac ggtggcaatt
    11521 ccggccgtcc aggctgaagg gtacgaatac tactctaacc agggtgccct ggtttacggt
    11581 tcaaacttcg ggggacaaaa gaaaatcatt gccaagttta cgggaattaa cagccgcgat
    11641 ttgtaccctg tggaaaatat ctctggcgag caggaacgat atattatcga agatgaagtc
    11701 accacaatta aagaggagta ccactggata acggacggtc acgtcattct ctggcgaggg
    11761 aaaatcgtga gtaatcctcc cggtacgcca acggtcgatg ttgccagctt taaggctctg
    11821 ggccgcttcg ccgtcgataa atacagtctc tattttgacg gtcagcgcac cgaaagcaat
    11881 agcggcgctt cgaaggtgga cctggcaacc ctaaaagcta tcgaagataa cagcagcacg
    11941 ctggttgacc gacataacct ttatttattg ggacgcaggc aggccagtag cgacgatttc
    12001 agcgtactac aaagtaaatt gtggggggat atcgagcggt tccgcccacg caatgaaggg
    12061 gctacctccc gcgacctgct tattcgtcat cgccaggatg tgtttctcaa tggtcagcgc
    12121 attgcagacg ttgatgctga cacctttcac atcattcgat ggatccccaa ttcattattg
    12181 gtctaccgcg acaaacatgg tgagcaccgt tacgcctatg gcaacccggc aggaaaaatg
    12241 gcaacgaccg atgatgcgaa ttcgtttgaa atcggtgaaa aaaccgtccg ctggcgcagg
    12301 caactcgcgc gggatgacgt ctggcacacg caactctcgt gggatgtcat atggggggcc
    12361 tggcaggata tccccaatgt tgatccggaa cagttccatt tgatcactga ccgcatcgcg
    12421 cagtatcagg atcgtctgta tatcatcaaa ctgtcgccag ttggcgaaga ccagcttaac
    12481 gttatcaccc tggatacccc tgatttagtg gttgatcacg tcttcaatgg tgggaaaaag
    12541 cacatttata ttatcaagga cagaacgtgg gtgcaggatg ttcatgtgat tgctacccac
    12601 ggtccactca ccatggctga gggtttcgcc tgggacgatc gctatgtata tacctggtgg
    12661 ggccagcgtc cttccagaac tgagtcgcca tgtccggagc aaaccgttga gcaggatgat
    12721 ggcatagtca ttaagaccga agcatcagag tgccaccgct cgccgtaaaa tgggtcatta
    12781 tttttaacgg gtagtttcat gatcaacacc tcgcgccagg atggctttat gcactatcgc
    12841 aatgtggttt gtttgttggc ttgttcattg tttttatcgt cggcatgggg atgtcggctt
    12901 tccgcacctg aacataatat ttaccagaaa cagggaaaag gcgtggtgta cctgcgccct
    12961 tataaagaaa caaatctttc tctacctgac gtgaattaca agagtttgcg tcggttaccc
    13021 aatttattaa tagacccgac aacactagac gaatgggata aagaaccgcc tctgactgat
    13081 ttgacaaccg actacctcta tgagggcgca caggcctggt atcctcatta ctcctggcat
    13141 agcgatggtc gctatatcct ctacgcgggt gaagttgtgc aaaacccgcc agacaaacct
    13201 cccgtcgatg tcgcctcgtt caaggcgtgg ggtgattttg ccgcagataa acacagtctc
    13261 tatttcgaag gcaagcgcac cgataacaat ggcgggggaa atcgcttaga tatcaaaaca
    13321 ctgcatcagg tggaattccg gcctccctgg gatcctgact tactggggct gatattacgt
    13381 gacgctaatt tcttgtatat caatgggcat cgcctcgctg acctcgagag tttccgtgtg
    13441 ctggcgcaga agtcctggga tcagcgagga aagttttcca ccacgtttaa cccctgcatt
    13501 gccgtaccat ttggcccctg ggataccctg gctcgtacac ggaccaaaat cctgatcaac
    13561 ggcgaacagc ttgatgccga cccggacacc ttttccgtcg tgcgctggat gcccggctca
    13621 ctcttgacct ggcgtgataa aaacgggcta cagcgtaaag tcctcgacaa ggaaaatctg
    13681 gcgtgggatg aagatttaac aaagcactgt ctggattttt ctctgctgga aaagaaagtg
    13741 ttctggcgta aaggacctgc ttgtaaacag gaagaattac ccggactcga tccggagcag
    13801 tttcaaccca tcagtgatgc tgtcgcccag tatcaggact cgctttatac catcatcgaa
    13861 acagagtctg gtgaccgcaa gctggaaatc gtgaaacttg atgatcccaa tcttattatc
    13921 aacaaacgtt tcaacgccgg gaaacgccac ggctatttac ttacgcgtgc cgaagggtgg
    13981 ccataccatt ccggtttaca tgtgtttgaa tctgacggac cgctgatctt actggataac
    14041 cgttctccgg atgaacgcga agcccatctt aatgaccatc cctttttgcg cagatggtat
    14101 gcccgcgata accgctacat ttacagcttt gatggcgcgc agctctggcg ataccgcacc
    14161 gctgatccga aacaagttcg cttaatctgg aaggaacaac attcgggata tggctatggc
    14221 gtaaattaca aaacgggata tctggacgga aaaattactg atgacggcga atttattcct
    14281 gccccgcgca atgaggcgac aaaatgaagg gtcaaataat cacagttgca ctcatcctgt
    14341 tgggacacac atttctgacg cctgctgtac aggccatagg gttcgactat tacaatgatc
    14401 acggtgttat gtcttacggt aaaggttatt ggggggataa gaaaatcatt gcccagtttc
    14461 cggagatgag cagggccgat ttgcgccttg tgaaaaatat ctctgggaaa acgggttata
    14521 taccttccga tgaaataagc ataaaaaatg aggaatacca ctgggtgaca gacggccgcg
    14581 ttattctctg gcgtggcaaa atcgttagca atccaccggg aacccccact gtcgatattg
    14641 ccagctttca ggctatgggc cgcttcgcgg tcgataaata tagcctctat tttgacggac
    14701 agcgcaccga aagtaatagc ggcgcgtcac gtgttgatct ggcgacacta aaagctatcg
    14761 aaggtaactc taccacgctg gtggatagta aaaatctcta tttgtccggt cgacgtcagg
    14821 ggagcagcag taatgttact gtattagaaa aaagatggtg gggtattaat ccacgtctta
    14881 tgagtgtaaa cagaaatttg tattccaatg atctgcttat ccgcagtggg cagaatattt
    14941 atttaaatgg cgttcacctt acggcgaatg cagactcatt tgagataatt cgctggatac
    15001 ctcattcact gctggttttt cgcgacaata agggtatgca tcgttatccc tttggtcaat
    15061 tatcaggcaa agcgataccc gtagatgatg acgtctcttt tgaagtaggg gaaagtcgcg
    15121 ttcgctggcg taaacagctc acgtccgacc gtcagtggag caagtggata gacctaccag
    15181 gtattgaacc tgaacaattt catctgatta ctggcaatat tgcgcaatat aaagatcggc
    15241 tgtatgtaac aaaattatcg atatttggtg aagaccagct tgagataatc ccgctggata
    15301 cgccagacct ggtcattgat cgctcattta atggcggcaa acaacatgct tactttatcc
    15361 gccaattacg gtcaaagagc ttgcaaatta ttccagttaa cggtccgcta actaaaaacg
    15421 atcgcttcgc ttatgacgat cgcaatgttt atacatggac cgatacagag gtaaggatta
    15481 cgccctcccc ctgcccggcg aaaactcgtg tcagagagga gaacgtacgt gaagttcaaa
    15541 acagagacat cattattccg gtgacggatg aatcatgccg gaacgcagca gcagaggtgc
    15601 aaactttgaa gccctgaccc catgccgcta tactgccgcc atcacttaaa aacaggatac
    15661 aacaatgaac gacagtgaat ttcatcgcct ggctgatcaa ctgtggctga ccattgaaga
    15721 gcgtctggac gactgggatg gcgacagcga tatcgactgc gaaatcaacg gcggcgtgct
    15781 gaccattacc tttgaaaacg gcagcaaaat cattatcaac cgccaggaac cgctgcacca
    15841 ggtatggctg gcaaccaaac aaggcggcta ccattttgat ctgaaaggcg atgagtggat
    15901 ttgcgatcgc agcggcgaaa ccttctggga tttgctggaa caggcggcga cgcagcaggc
    15961 gggtgaaaca gtcagtttcc gctaagattg catgccggat aagcctcgct ttccggcacg
    16021 ttcatcacga aaaatattgc tgtaatagcg gcgtatcgtg atcctgattg gcaggcggca
    16081 tgttaccgat agattttgtg cggaacggaa tcacctgttc acgaccatca accttcacaa
    16141 tctgatagaa ctgcggcagg ttgaagttga tgaagcttga gccgtaggtg aagcggtcgt
    16201 gcgatgacga gtagaagcga ctgacgtcac gcaccagttc ctctttgctg ccttcgcagt
    16261 ggtgatacac ctcaacccgg ttgctttcgt caagaatgta gatattaaag ccattctcgt
    16321 cttgcgtttc ttcgaagaag aactggatga tcccttcact agcaaagccg tccaccaccg
    16381 ccggtaattt gacgtgattg gtttcaacct gcactgacag gccgtgcagt ttgttatgcg
    16441 aaatagcgcc ataaaactcg atggcgtttt ccagtttttg taccgataca ttcaggcgtt
    16501 cgaagaacaa cccccaggtt tgaccagaaa cgcgcagcgc cttgaaacgc ccggtttcct
    16561 ggcgggtgct ggaaaggcgc agttcaatac actcagaaac cagttgctgc acgcgagtac
    16621 ggattaaccc gcgcagatgc tggctataac agaagacttc cacgctatct ggcggtgcgg
    16681 cgtcctgatg cattttgccg agaatagttt tcagggcttc gatcatcgat tgctcgccgt
    16741 tgaagtgcag cgtacgcact tcgttccacg agttgcggta cagcaggtca acgctaccta
    16801 ccaggcaatt ttgattctcg ccaaagctga agacatccag cttacggaaa tcaaaatgca
    16861 ccacctgatt gcggaacgcc gctgtcgggt catattccag gttaacgata atcgccagat
    16921 ggcggatctc acacgggctg tagagcgcct tcggtgtcgg tgcaggtaag cgcagcggga
    16981 aatggtgcga cacgtcggcg accatctcct gcaacttagg caaatcgaca atgccgttac
    17041 ctttaatata caaacgggtg cgcgaggtca gcaggccgtt aaaccatgcc cacgccacca
    17101 gtttgttcag gtaacggtta tattccagcg gctgatggct gatgatcgac tcaatatttg
    17161 gcgcgcggtt atacagatac caacctgaac ggttagcacg gcccggcggc acataaataa
    17221 aggtcagatt cggttccgag agatcgggtg aaatctgcgg gtttaccagc gtcactttac
    17281 ctggtaatgc ttcaaacgcg gcatacagct tacgcgtcag cacgccgata tcctgcggac
    17341 tggcggagac gctaaggtta ttgcgacgcg caaagcggat cagattacgg tagctctgca
    17401 tcatcgcgtc gagcaactcg ttgtgcgcct cacgcacctg atcaatcttc cagttagcgc
    17461 ggttatcgag cattgccaga cgagcttcgt cccaacccca ctcgctcact aactggctca
    17521 acactgcgcg acgccagcct acgcaggcgc gttcacggct gagcttttcg cacactttta
    17581 aataaaagca gcgacgtact aaatccagac gggtaaaatc ttcaatcgcc gtcaggtatt
    17641 cagtaacacg ctccagcatc atgcagtatg gatcgagacc aaacgataca atctcgccgt
    17701 cgtgcaaacg ctgtttgata tctttcgcca gcagacgtgg gttcgggtat tcccaggaat
    17761 aggcttccag cagcagtgtt ttcagtaccg ctttgtatgg ggaatcgata ctcttgtaga
    17821 gctgccaaag gctggcacca aagtactctt cagcagaaag cgagcttaag ccaccgagat
    17881 ccagccattc atttggcgtc agcacgccct gcgcgtaaag cgtcatcaca tagtcgtcgt
    17941 aatgctcttc ttcgtcgcac ggcaccatat tccacagaat acgcttaccg gcgagacgca
    18001 cggcggtacg ataaaattcg tcaagcagca gtatatgctg ggtggagcca caatcttcgc
    18061 cccccaggct gccgctttca ttatgacgga agcggttttc atcaatcagg aagaagctga
    18121 cttccacacc cagcgaggcg gcccagcttt ccagcagact acatttacgt tgtagtaact
    18181 ggcgctcttc gctatcgagc caggattgat gacagaccca gatatccagg tcagaggaac
    18241 aactttgccc tacggacgag gtgctgccca tggtgtatac accagtaatt ggaagctcac
    18301 ctttcggtgg gtcctgtact gacattccac gatacagttc aagctcgttc aggtagtggc
    18361 gttgagtttc atcaggcgtg taaaggcaaa tgcctttggg aacgttacca tcaaggtaac
    18421 ccggcattag cggatggtga tagtgcaaca atgtcggcag tagactgtag acctgttgga
    18481 atgcaggccc catagcagca agcgcgcgat ccacacgcaa ttgatttatg gcatccagtc
    18541 tctgtttcag agtctcaata tagaggtaca agacgtatcg cctgatttgc tacccgtcat
    18601 gactgtgatt ccgccaacat caacggtaac acgcggcatt cgggatattt cgtatgtcaa
    18661 aggtaaccgt taccactttt cgcgcctggt ttttttagtt tcacgacgaa aaaatggtct
    18721 aaaacgtgat caatttaaca ccttgctgat tgaccgtaaa gaaagatgcg ctacatacaa
    18781 gtgtagcacc gtttattctc tgtaaattcc ttattacgac ggcgtgaaac gcctgtcagg
    18841 atccactgcc agacctcatt ttacggtttg cgcaggcgtc tacgtttcac cacaacactg
    18901 acatcactct ggcaaggatg ttaggatgga ccacggatga taatgacggt aacaagcatg
    18961 ttagacaatg ttttaagaat tgccacacgc caaagcccac ttgcactctg gcaggcacac
    19021 tatgtcaaag acaagttgat ggcgagccat ccgggcctgg tggttgaact ggtacctatg
    19081 gtgacgcgcg gcgatgtgat tcttgatacg ccgctggcga aagtaggcgg aaaaggctta
    19141 tttgtaaaag agctggaagt cgcgctcctg gagaatcgcg ccgatatcgc cgtacattca
    19201 atgaaagatg tgccggttga attcccgcaa ggtctggggt tggtcactat ttgtgagcgt
    19261 gaagatcctc gcgatgcctt tgtgtccaat aattatgaca gtctggatgc gttaccggca
    19321 ggcagtatcg tcgggacgtc cagtttacgt cgccagtgcc aactggctga acgccgcccg
    19381 gatctgatta tccgctccct gcgcggcaac gtcggcactc gcctgagcaa actagataac
    19441 ggcgaatacg atgccatcat tcttgccgta gccggactaa aacgtttagg tctggagtca
    19501 cgtattcgcg ccgcgttgcc acccgagatt tctcttccgg cggtagggca aggtgcggtg
    19561 ggtattgaat gccgccttga tgatgcacgc actcgcgagc tgcttgccgc gctgaatcac
    19621 cacgaaactg cactgcgcgt taccgcagaa cgcgccatga atacccgtct cgaaggcgga
    19681 tgtcaggtgc caattggtag ctacgccgag cttattgatg gtgaaatctg gctgcgtgcg
    19741 ctggtcggcg cgccggacgg ttcgcagatt attcgcggtg aacgccgcgg tgcgccgcaa
    19801 gatgccgaac aaatggggat ttcgctggca gaagagctac tgaataacgg cgcgcgcgag
    19861 atcctcgctg aagtctataa cggagacgcc ccgacatgag tatcctggtc acccgcccgt
    19921 ctcccgctgg agaagagtta gtgagccgtc tgcgcacact ggggcaggtg gcctggcatt
    19981 ttccgctgat tgagttttct ccgggtcgac aattaccgca acttgctgat caactggcgg
    20041 cgctggggga gagcgatctg ttgtttgccc tctcgcaaca cgcggttgct tttgcccaat
    20101 cacagctgca tcagcaagat cgtaaatggc cccgactacc tgattatttc gccattggac
    20161 gcaccaccgc actggcgcta cataccgtaa gcggacagaa gattctctac ccgcaggatc
    20221 gggaaatcag cgaagtcttg ctacaattac ctgaattaca aaatattgcg ggcaaacgtg
    20281 cgctgatatt acgtggcaat ggcggtcgtg agctaattgg ggataccctg acagcgcgcg
    20341 gtgctgaggt cactttttgt gaatgttatc aacgatgcgc aatccattac gatggtgcag
    20401 aagaagcgat gcgctggcaa tcccgcgagg tgacgacggt cgttgttacc agcggtgaaa
    20461 tgttgcagca actctggtcg ctgatcccac aatggtatcg tgagcactgg ttactacgct
    20521 gtcgactatt ggtcgtcagt gagcgtttgg cgaaactcgc ccgggaactg ggctggcaag
    20581 acattaaggt cgccgataac gctgacaacg atgcgctttt acgggcatta caataactct
    20641 cataacagga agccataatg acggaacaag aaaaaacctc cgccgtggtt gaagagacca
    20701 gggaggccgt ggacaccacg tcacaacctg tcgcaacaga aaaaaagagt aagaacaata
    20761 ccgcattgat tctcagcgcg gtggctatcg ctattgctct ggctgcgggc gtcggtttgt
    20821 atggctgggg taaacaacag gccgtcaatc agaccgccac cagcgatgcc ctggctaacc
    20881 aactgacggc attgcaaaaa gcccaggaga gccaaaaagc cgagctggaa ggcattatta
    20941 agcaacaagc tgcacagctt aagcaggcga atcgtcagca agaaacgctg gcaaaacagt
    21001 tggatgaagt ccaacaaaag gtcgccacca tttccggcag cgatgctaaa acctggctgc
    21061 tggctcaggc cgattttctg gtgaaactcg ccggacggaa gctgtggagc gatcaggacg
    21121 tcacgaccgc tgcagcgttg ctgaaaagtg cagatgccag cctggcggat atgaatgacc
    21181 cgagtctgat taccgttcgt cgggcaatta ccgatgatat cgccagcctt tctgcagttt
    21241 cgcaggtgga ttatgacggc atcatcctta agcttaatca gctttcaaat caggtagata
    21301 acctgcgtct ggccgataat gacagcgatg gttcgccgat ggattccgac ggtgaagagc
    21361 tttccagttc catcagcgaa tggcgtatca atctgcaaaa aagctggcag aactttatgg
    21421 acaacttcat tacgattcgc cgtcgtgatg acaccgccgt accgctgtta gcgccaaatc
    21481 aggatatcta tctgcgcgaa aatattcgct ctcgcctgct ggtcgcagca caagctgtac
    21541 cgcgtcacca ggaagagact tatcgccagg cgctggagaa cgtctccacc tgggtacgtg
    21601 cttactacga tactgatgat gccaccacca aagcgttcct cgacgaggtg gaccagttaa
    21661 gccagcaaaa tatctcgatg gatcttccgg aaaccctgca aagccaggcg atgctggaaa
    21721 aaatgatgca gacccgcgtg cgtaacctgc tggcacaacc ggcagcgggg acaacggaag
    21781 ctaaacctgc acctgcacct gcacctgcac ctgcaccgca aggtgatact ccggcagccg
    21841 cgccgcaagg agaataacca tgctaaaagt gttattactc tttgttttgc tgattgcggg
    21901 gatagtggtt ggcccgatga ttgccggtca tcagggttac gtgctgatcc agactgacaa
    21961 ctacaatatc gaaaccagcg tcacgggcct ggcgattatt ttgattctgg cgatggtagt
    22021 gctgtttgcc attgagtggc tactgcggcg gatcttccgc acaggcgcac acacccgtgg
    22081 gtggtttgtc ggacgtaagc gtcgccgtgc ccgtaagcag accgaacagg cgctgctgaa
    22141 actggcggaa ggcgattatc agcaagttga aaagctgatg gcgaaaaatg ccgatcacgc
    22201 ggaacaaccg gtggtgaact atctactggc tgccgaagcc gcgcaacaac gtggtgatga
    22261 agcacgcgcc aaccaacatc tggaacgcgc agcagagctg gccggcaatg acaccattcc
    22321 ggtagaaatc acccgtgtac gtctgcaact ggcccgtaat gaaaaccatg ctgcacgcca
    22381 cggcgtggat aagctgctgg aagttacgcc acgccatccg gaagtattac gtctggcgga
    22441 acaggcgtat atccgcacag gtgcatggag ttcgctgctg gatatcatcc catcaatggc
    22501 gaaagcccat gttggtgatg aagaacatcg tgcaatgctg gaacaacagg catggattgg
    22561 cctgatggat caggcgcgtg ccgataacgg tagtgaaggt ttgcgtaact ggtggaaaaa
    22621 ccaaagccgg aaaacgcgtc atcaggtagc gttgcaggtg gcaatggcgg aacatcttat
    22681 tgaatgtgac gatcatgata ctgcccagca aattatcatc gatggcctga aacgccagta
    22741 cgacgatcgc ctactgctgc cgattcctcg actgaaaacc aacaatccgg aacagctgga
    22801 aaaagtgctg cgccagcaga tcaaaaacgt cggcgatcgc ccgctgttgt ggagcacact
    22861 gggccagtca ctgatgaagc acggagaatg gcaggaagca tcgctcgcct tccgcgcggc
    22921 gctgaaacaa cgtccggacg cctacgatta cgcatggctt gccgacgcgc tggacagact
    22981 gcacaagccg gaagaagctg cagcgatgcg tcgcgacggt ttgatgttaa cgttgcagaa
    23041 caacccacca cagtagttcc ttctcacccg gaggcaagca cctccgggac ctttctgatg
    23101 cataaaaaaa cgcctgctct tattacggag caggcgttaa aacaggtctg tatgacaaca
    23161 agtgggtgct tcactcaacg ttgtgtccat ggtgtctgat gaggcataag cgacatctgt
    23221 cagtggacga taagcaccgt aaacggctct gcgtcattcc ggagtttatg aggcactaag
    23281 gcgaacataa gagatggaat gagcatctac ccgtttatta tgccacagag aatcgggaaa
    23341 taacatccct taacacttgt tatgagataa ttctgtaatc ctctttgctt cctgagtaat
    23401 aacttcctga gtgaatattt aacctgagct tgatcctaca catacttatt atgaatgata
    23461 aaattcattc aattaataac acatatatta attgccgtta aaactaaaaa aagcaccaat
    23521 aatcaacgcg atataataaa cctgccttac atatcaactg cgccagaggt aggattgaaa
    23581 acgctctcct gattttccaa ttcattttct ggataaataa ataatttatt tttgtcacta
    23641 ttatttatgt aatcatcctg tcagggagag ggatctcaat tatcaatgct taattacgtc
    23701 atcattttga tgacatgaaa ccggagcatt ttgcatggaa ttttcgtttt cacccaaact
    23761 tcttgttgtt gctgtcgccg ccgctcttcc tctcatggcc agcgcagcag ataccccgtc
    23821 aactgccacc gcacgcaaag gctttgccgg atacgatcac ccaaaccagt atccacagaa
    23881 cgctgcaatt tattgaattt gcacgatcat gtaggccgga taaggcgttt acgccgcatc
    23941 cggcaatcaa ccgcaggcgg ccacaaaatg gccgcttttt tacttattca ccaccagcaa
    24001 atgtgcctgc ataatgtcac tggccgagcg accgtgcgcc agcaaatcag ccattgcttt
    24061 aagatatggc ggtagatggc ggaaataacg ctgatacccg gcacacaaat aattcagccc
    24121 cggtttgccg ctggcatcga gcatgaagcg gtgtttcggg cagcctcccc agcacgcttt
    24181 taacacgtta caactgcgac actgcgccgg taattgctta aatttatctt caccaaacac
    24241 ctgctgttgc ggggaatcga tcatttctgc aattgtttgc tgatgcatat tccccagccg
    24301 atattgcgga taaacatagt gatcgcaggc gtaaacgtcg ccgttgtgct caacaatcac
    24361 cgagcgccca caggttggct gatgatggca aaccgcaccc ggcgcaccga caaaattggc
    24421 aaacgcccat tcgatattca tcacgaaaat cttgccgacg tcgcgtttga tccagtggtc
    24481 gaatatcgcc accagaaatt caccaaattc atcggggcgc accgaccatt ccgtcagttc
    24541 cccctgaata tcaccaggcg catgtagttt cagtccatcg ctggcagctg tttcatcagc
    24601 caggcgctcg accaccggaa taaactggat gaattcgact cccgcatcgc acaaaaaatc
    24661 ataaacctgc aacggttgct gcgcgctggt gcggttgacg cagaccagca cgttatagtc
    24721 gacatgatgt ttttgcagca gcgtcagggc acgcatcacc agcttatgcg ttggtctgcc
    24781 acctttggtc acgcgatatt gattgtggat ctcagccgga ccgtccagcg ataacccaac
    24841 aagaaaatga ttttctgcca gaaatgcaca ccatttatca tcgagcagca cgccgttagt
    24901 ctggaagctg ttacttatct tcctgccagc accgtatttc gcctgtaact ttacggcacg
    24961 gcggtaaaaa tccagcccga gtaacgttgg ttcgccgccc tgccaggtaa aagccacttc
    25021 gttttgcggt tcgctggcag cgatatagtg acggacatac gcttccagcg tgtcatcgtc
    25081 catatgcgtg actggctttt cgcggtaaag ggattgtttt tcgagataaa aacagtagtc
    25141 acagttcaga ttacaatcgg aaccactcgg tttcgccatc acatgaaaag cacgcgttgg
    25201 aacctgttgc agcatggtca ctccttgcct gaagtcatcc ttcaatacgt taagggcgaa
    25261 atcataccgt ggggcaatga gttattacgg gcgtttattg cgaagggagg gtgacgaaat
    25321 gcacagaaaa caaaaaaccc cgccgaagcg gggttcaaaa ttggtcggcg agagaggatt
    25381 cgaacctccg acccactggt cccaaaccag ttgcgctacc aagctgcgct actcgccgaa
    25441 atactgcttt ttgaattttt agttcaattc tttaaagtcg tggtgcgagg ggggggactt
    25501 gaacccccac gtccgtaagg acactaacac ctgaagctag cgcgtctacc aattccgcca
    25561 ccttcgcatt gtcacaactt ctaataatgg ggtggctaat gggattcgaa cccacgacaa
    25621 ctggaatcac aatccagggc tctaccaact gagctatagc caccactaca aatcttgtta
    25681 cgcggtatta ctaccaccgc agctcaagcg ccgggactaa atggcgcgcc cgacaggatt
    25741 cgaacctgag acctctgcct ccggagggca gcgctctatc cagctgagct acgggcgctt
    25801 agcgccgttg cgggggtgga taatacggac ttcccacccc tctgtctagt ccctttttaa
    25861 ataaattgcg cgtttggtta cggtttgtgc gttttgctgc ttattcctcc agtttatgcg
    25921 ctttcccgtg gcgattaagg ccaaaaactt tataaatcgc cgtcaccgcc agcataaaga
    25981 tgatgccaac aaacagcgac atacgcgtat cttcattaaa gtacatgccg atcaaaacgc
    26041 aaatcaggaa tgccattgtt acgtagttgg cccacgggaa caggattgag cggaacggat
    26101 ggctggcaat cgccgcttta tgtgcacgcc gaaaacgcag ctggcttatc aatatcacaa
    26161 accatggcac catccccgga agcacgctgg cactgtagac gtagacaaac acacgctgcg
    26221 gattgggaat gatgtagttc aggcatgagc caattagcag aatagcaata gatactgcca
    26281 cacccgcaac gggtacgccg tgacgggaaa ctttcgccat cgccgccggt aactgacggt
    26341 ttttcgccag tgcgtagagc atacgtccgc aactgtacat gccgctgtta cagccagaga
    26401 gcgcagccgt cagcaccaca aagttgataa tgcccgccgc tgcggtaata ccgattttgg
    26461 caaaagtcag tacgaacggg ctgccgttgc tgcctatttc attccacggg aagatggtga
    26521 cgataacgaa aatcgcgcct acgtagaaaa tcaggatccg ccacagcacc ttgcctacgg
    26581 cactgcgcag cgtcacctgc ggattcttcg cttcaccggc agtaatgcca atcagctcca
    26641 cgccctggta ggacgccacc acaatacaca gcgcggtcag gaaccctttc cagccacccg
    26701 caaagaaacc gccatgctct gtgagattgc taaaaccaat cgactgcccg ccattgccaa
    26761 agccaaagaa aatcacgccc aggccaatga caatcatcac gataatcgtg gtgactttga
    26821 tcatcgcgaa ccagaactcg atttcgccgt acaaccgcac cgccgccaga ttcgccaacg
    26881 ccaccagcgc cactgcgatc aatgcgggta tccactgcgc catctccggg aaccagaact
    26941 ggacataaac gccaatggcg gtgatttctg agatccccac ggccatccac ataaaccaat
    27001 aagaccaggc ggtgagataa ccaaagaacg ggctcatata acgatgcgca taaacggcga
    27061 acgaaccggt aaccggttcg aggaacaaca tttcgcccat tgaacgcatg atgaaaaaga
    27121 cgaatagccc ggcgatgata taggccaaca atacggatgg cccggcccat ttcaaggtac
    27181 tggcggcccc cataaacagg ccgacgccaa tggtgccccc cagggcgatg agttcgatat
    27241 gtcgagcttc cagcccacgc tgtagctctg gtttgttatc tgccataaat cctcgtgttg
    27301 tgtttgcatg ctttccggtg ttaccggtta tcgttatggg tacatcgagt gttgcgaatg
    27361 ttttcgtaat tcaggagaaa tggcaaataa agcattaaaa atttgaatgc tttgtgtaat
    27421 aaaaaagcag acaggcgacg gagtgaccac tccgtcgctt tacaaagaga ggaaaatcag
    27481 aggttgccgg tgtagtgcca gcgtaaataa cgcagcaaac gaagctgacg cttaatgcgg
    27541 ctcggctgcg aaagcaggcg gtagagccac tccagtccca gcgtttgcca gattttcggt
    27601 gcgcgtttta cgtgaccggt gaaaacatcg taagtcccgc caacgcccat atacagcgca
    27661 tctggatgca ccagacggca gtcgcgcatg aagatctcct gctttggcga tcccatcgca
    27721 acggtgacga tttgcgctcc gctggcatga atgcgttcaa acagcgcctg acgctgctcg
    27781 ggtttaaaat aaccatcctg actgccaacg atattcacat tccactggtt gcgcagttta
    27841 gcttcagttt gcgccagcac ttcaggttta ccgcccacaa gaaataccgg cgtcccttct
    27901 ttgcctgcgc gcgccatcag ctcttcccag agatcggcac cggcaacgcg ggaaacctgc
    27961 gcctgcgggt actttttacg tactgaacgt acaacgctga tgccatccgc atatttaaat
    28021 tcggcagcgt taattaactc cctgacctcg gcgttatctt caatggtcag cattttttca
    28081 gcattaatgg caaccagcgt tccctgctta agctgcccgt cagcaaacag ataatcgagg
    28141 gcgtgctgca tatcacgcca accaatcaac tgtaagccac gcagcgtata ggtcggtgcc
    28201 gtggtgttgt tattcattgt tatccttcaa cctgcgtccg gagcgatgat tttgtacgtt
    28261 tatgaatgag tccggcgctt tcaaaaagcc agtacaacag ttttgcgatc atcagacatg
    28321 cgccgaagac cacgataaaa aagactacgc gtgagacaaa cgaatccagc ccttcacgtg
    28381 ccagcacgat catattgaag atcgccccaa agcagaaact gtgcaatatc gcagccttat
    28441 agcgattagg ctcgcggttg cccagctcat acagccagtc gaaccatttg atgatcagac
    28501 caaccacgat cgccccgagc gggatgaaca acgcgccgcc catcaccacc agtgagccta
    28561 taagcgtagg cgagatcgcc agtccggagt ggttattcag cacttcccag gtaaagtagt
    28621 tggctgagtt cagcaccata ctcgggcgac ccggccacag ccaggaaggg ataaagacat
    28681 agaaatcgcg gacaattgga gccaggccct ggaagtcgat gttgtcgtag ttctgcaaca
    28741 gcaacgccag attctcccac ggcgagaagg tgtcgcgagt gagatagaga aacgtataga
    28801 acgcttcatc gccgctcaca ttcattccat agcgttttag cgccagccag aacatgccca
    28861 caatccccag cacgcccgcc gccgccagca tccacaacga aatccagccg cgaataatgc
    28921 caataaacag gaagatagcg aatgcgatga tgatattggc gcgagtgccg ccgacaatca
    28981 tataagtcag caagccaaag gcgaccgtgc tgacgaggaa aaacagccac gctttgctat
    29041 cctggcgcag aaagtagacc accagcatcg ccgggatgaa aaagtaaaag aagcgtttta
    29101 acgccacgcc ggagacttca ctggagaaga tctgactgta ggagttaagc cggaacagca
    29161 aaaagccgtt gtgcatgaag aagatgccga cgcttaccag cgcgatgccc atcaggatca
    29221 cccacgtaag attggtctcc acgcggttca tggtaaacag cggacggcgc ggtacatcag
    29281 caacgcgttt gcgtaggcgg gttttgtagg tgacatagta aaccgcgtag aagcagcccg
    29341 cagaaagcaa cgcctgcaac aagatttctg gcggcgcgac accaacatca aagcgaaata
    29401 ccagcacgct ggtcagcggg aagccgaaga aaaaggtgag caaaaacagc aatgaaaaga
    29461 agacattgaa gttaaagcgc acacggcgaa actcaaacca ggtcagcgtg gcaataaaca
    29521 gcgtgcagag cagccagaca acaaacaggc cactgaattg cagcagactc atgcgacctc
    29581 cccggcggca atcgccaacg cccgctgcca gccttgtaga tagttagggc taaagaaggc
    29641 aatggtgttt ttatccaccg acgccaactg gcgctgcgct tcacgcacaa tatcctcgtt
    29701 gagatcgtcg gtagtaaaca gcaccggcaa atgctgttcc gtcatatcct gccagaacgg
    29761 attttcccgg ttaagcacac aaggaatgcc cgcctgaatc agtaagcaaa gcgtaccaat
    29821 gccctgctgg cgggcaaaaa taaagtaacc aagatcgcac tgacgaagta gcgccagata
    29881 ggcgtcaaat tccagttttt cgctcaaaat ttgcagattt tcttcgctga ataactccag
    29941 ccccgcctga cgaacttcct caatgtacgc ttcgttatta ggcggatatc ccatcggcac
    30001 caccactttt accgtatcgc caaattgctg atgaacggcg cacaaggcag caatatggtc
    30061 attgctgcgg tcaccggagt tccccaccag aatggtcatt ttcccttcac gttgccgatc
    30121 gttcgccatc gtattgagag aagggtccat ccgcgtcggg aagtacagca gttcgcccca
    30181 cacctttgga tgcgttttgg caaaaaagct caaatcgccg cgggtggcaa atacacagcc
    30241 gactcgcttt tgcgccaggc gacgtagtgg gtaaaaaagc ttatatctca agccactgga
    30301 aagctcatac aggtctgccc cccagatatg ccagaaaaac tggctgggct taatgccacc
    30361 actcagcaga gccagccaca gtgtgggatt gaactgaccg tggaagaaaa aacgctgctg
    30421 acggttagct tttgctttcg cgatgaccgc ttccgccagc gattttttcc cagggaaaaa
    30481 ttgcacagaa agcgccggac agctatcact taagccgtcg tccttgccaa caaccataaa
    30541 ctcgcgcgcg tgctcgctcg tcgcggccag cgcgtcattg aaaaaccgca aaacggttcg
    30601 gttatggtga gggatatccg atcccagtac gtgaatcagt acagtcatgc ccgcctacgc
    30661 cagagtaaaa acacgccaca acaaagagaa aaatagacga tataagttgc catatatgcc
    30721 tgcgccgcgc ccagtgcacc atgcgcaggg attagccagt gggcaaatac catcaataaa
    30781 gtgaactggc tgacttccgc cagaatataa aaccgcagtg acgctttggc gatcaccaga
    30841 taaccaaaga cataagcgcc cacttttaac acatcaccca ctaactgcca ggcaaagaga
    30901 tcgcgcatag cggtaaattt attcgacaac agcagccaga tagcaaaatc acgcagcagc
    30961 cagacggtaa aactcgccgc cgccactgcc ggtaagacga atttcagcga tttaaccact
    31021 tcccgggtga tatcgcgctt ttccgttagc cgcgacaacg tgggcagcaa atatacgctg
    31081 aacgatgccg taataaattg caggtaggca tcggaaatac tgctcacccc ttgccagatc
    31141 cccacttcat cccagctata ctgcgccgcc agcagtttac gcatcatgat gtaagcaaca
    31201 ggcaaggtca ccgacgtaat caacgccatg agcgtaaatt tgctcaactg ccctgccaga
    31261 ccgttatccc agctgggttt cagatagctt aacgggatga caccacgttt gatcaacatg
    31321 atggcggcag gaattaccac cagcgcggga atcagcgcca gacccagcaa cgccccttca
    31381 taaccgccca aacggtaact gacgtagtac gcgagaacgc caatcaagct gccgacaatc
    31441 agcgataacg cattacctgc ggcatcgcga aagcctttca tcagcgccag taacaggttg
    31501 ccccaggcga tccccatttg caccagcgcc actaaacgca ccagcccctg atagtcggta
    31561 ttaccaaaca atccctggct gattggcgca gctgccagca caaaaaccag cgccatcagc
    31621 gtagagaaac caagtaccat cgctgatgaa gtgccgacca cgcggcgcag ctgttgcgga
    31681 ttatcatggt actgggcaac gtatttggtt acaccgttaa agatgccagc tccggcaagc
    31741 acgccgagca cggtaatcaa ctggcggaaa tttgccgcca gcccaagccc cgccggacca
    31801 aatgacacag ccagcaactt accgaccagt aacccggcac caatcttgac cagtgtactg
    31861 gccgccgtcc acaaggacgc ttttgccaac gacatatcag gagaagtagt tcaacaaagt
    31921 cgcaattacc gtacgctgat tgacgggcga caggttgtag aacagcggca ggcgcagcag
    31981 gcgctcgctc tctttggtgg tgtagcgatc ttcaccgtgg aactcaccaa agcgttcccc
    32041 cgcagggcaa ccgtgcagcg gaatgtaatg gaacaccgcc atgatttccg cttctttcag
    32101 aaagttaatc aacgcgctcc ggtcatcaat atcccgcagt ttaatgtaga acatatgcgc
    32161 gttctgcacg cagccatcgg gaatcgacgg cagctcgata cgcccggctt tcgccagagg
    32221 cgctaacgca tcgtagtagt tttgccacag cgccagacgt tgctggttga tacgatccgc
    32281 tgcttccagt tgcgcccaca ggtacgcagc ttgcagatcg gacatcaaat agctggagcc
    32341 aatatcgcgc caggtatatt tatcgacctg accacggaag aactggctgc ggttagtgcc
    32401 cttttcacgg atgatctcgg ctcgttcgat taacgcttta tcgttaatca gcgtcgcgcc
    32461 gccttcacca cccgccgtat agtttttggt ttcatggaag ctaaagcagc caatatgacc
    32521 aatggttccc agtgcacgcc ctttgtaagt ggacatcacg ccctgagcgg catcttctac
    32581 cacaaacaga ttatgctttt tcgccaacgc cataatggtg tccatttcgc aggccacacc
    32641 cgcgtaatgg accggcacga taacgcgcgt tttgtcggtg atcgccgctt caatcagcgt
    32701 ttcgtcgatg ttcatggtgt ccgggcgaac atccacaaaa acgatttttg cgccacgcag
    32761 cacaaaggca ttggcggtgg agacaaaggt gtagctcggc atgatcactt catcgccagg
    32821 ctggatatcg agcagcagcg ccgccatctc cagcgaagcg gtgcaggacg gcgtcagtaa
    32881 cactttggcg ctgccaaaac gttgctccag ccactgctgg cagcgacggg taaaaccgcc
    32941 atcgccacac agtttgccgc tacccattgc cgactgcata tagtcgagtt cggttcccac
    33001 caccggcggt gcgttaaatg gaatcatgtg atcacctgta taaccagtac gcggtgcttt
    33061 ctacattcgc accactttgt atgtatcgtt taagcgcggc ggtgttgccc atttgggtcg
    33121 ccacccgcaa agttgtttta ccgcgagcat acgcccagtt tagcgccgtt tgcatcagct
    33181 cagcacctac accgcgtcca gccagcaggc caattcgcgc atctgtcgca ttgagttccc
    33241 gtaaagagac ataaccgcga ataccgccgg acgccgcacg taaaatcaga cattgatgat
    33301 caaaggtgcc gcgcacggca ttttcaatcc actgtgcata aaagcgtctg ctggcgtcag
    33361 gcgcatacca cggcgcacga aaacggcttt gcgcaaatgc ggcgctggct aactgacgta
    33421 atgcgggaat atcggtctct tgtgccacta cagcaccact atcactgaca ttgttcacgg
    33481 gtagtgccaa atcaacttca ccttctacca gggagaatcc cagctgttgc agggcatcca
    33541 gttcacccgt atttgatgcc gcaattttgg cctgcacccg tgaccacggc gctaacacgt
    33601 ctggcgtcag gagcggtgct tcagacgtaa tgcgcacgat ggcgctgtta acaccaaaga
    33661 aggcgttttc ccaggttagt ggctcaatac tggcgcggac gggcacgaag taactccagc
    33721 agatattggc cgtagccagt tttcgctaat gaactggcag cacgcttcac accctcgtca
    33781 tcgagccagc cgttacgcca ggcgatctct tccaggcagg caatcttaaa gccctggcgt
    33841 ttttctactg tctgcacaaa ggtactggct tcaatcaggc tgtcgtgagt gccagtgtcc
    33901 agccaggcaa atccgcgccc gagcagttca acggtcaggt tgcccgcctc gaggtacatc
    33961 tggttgatgg aggtaatctc cagttcacca cgctccgacg gcttcacctg ctttgcgtac
    34021 tccacgactt tactgtcgta gaaataaagc ccggtcaccg cccagtttga cttcggctgt
    34081 tttggctttt cttccagcga gatagcgcgg aaattgtcgt caaactccac cacgccaaag
    34141 cgttccgggt ccatcacctg atagccaaat accgtcgccc cttccgtgcg cgccgcaaca
    34201 tgacgcagct tcggactgaa gccctgaccg aagaagatgt tatcgcccag caccagacaa
    34261 gaaggttcac cgttgaggaa ggtttcaccg atgataaagg cctgcgccag accgtccggg
    34321 ctgggctgtt cggcgtattc cagctggata ccgaactcac taccatcgcc cagcaggcgc
    34381 tggaaataac ctttatcttc cggcgtagtg atgatgagaa tttcgcggat accggccaac
    34441 atcagcaccg acagcggata gtaaatcatt ggcttatcgt aaatcggcaa cagttgcttc
    34501 gatacgccgc gcgtaatcgg atgcaatcgg gtgccggaac cgcccgccag gataatacct
    34561 ttcatgtttg cctccgctga aaattagcct ttcagaccta aacgctcgcc ctgatagctg
    34621 ccgtcctgca cctgcttcca ccagctttca ttagccagat accactgcac cgttttacgc
    34681 attccacttt caaaggtttc ctgtggcagc caaccaagtt cacgggcaat tttcgaagca
    34741 tcaatggcat aacgcagatc atgccccgga cggtcagcga caaaggtgat caagtcacga
    34801 tagtgcacca cgccgtgcgg cttgttcgga gccagttctt ccagcagctc gcaaatggtt
    34861 tccacaacat cgagattctt acgctcgttg tgaccaccaa tattataggt ttcaccgact
    34921 ttcccggtgg tcgccacgca atacagcgcg cgggcgtgat cttccacata cagccagtca
    34981 cggatttgct gcccgttgcc atataccggc agcggtttac ccgccagcgc gttgaggatc
    35041 atcagcggga tcagtttttc cggaaagtgg taaggaccgt agttattcga gcagttggtg
    35101 atcagcgtcg gcagaccgta ggtccgcagc caggcgcgca ccagatggtc gctgctggct
    35161 tttgacgcgg aataagggct gctcggcgca tacggcgtgg tttcggtgaa gaaatcatcc
    35221 gtcgagtgca ggtcaccata tacttcgtcg gtggagatat gatgaaaacg gaacgctgat
    35281 tttttatctt ccgtcagcgt attccagtaa gcccgcgccg cttcaagcaa tgtataagtc
    35341 ccgacaatgt tggtttcaat aaacgctgcc gggccgtcaa tagaacggtc aacatggctt
    35401 tcggctgcca gatgcatgac acagtctggc tgatgctcag tgaatacgcg tgccagttct
    35461 gcccgatcgc agatatcaac tttctcaaag gcaaagtgct cgctttgcgc gaccggtgcc
    35521 agcgacatca ggtttccggc gtaggtcagc ttatcgacca ctaccaccgc atcgctcgtt
    35581 tcgttgatga tataacgcac cagcgccgag ccaataaacc cggcaccacc tgttatcaga
    35641 atttttctca tcagcgccag actcctttgg catcgacgac atactgctga tggacattgt
    35701 cgccattgat aactttgaac tgactatgat cgaccagcat caccagcacg tctgccgttg
    35761 ccagcgcctc gtcaagctgc gccagagtac aaagcccggt cagttttttc ggcaactggt
    35821 ggatgttagg ctcaacaacc agcgtttcgc cgctgtgcca ctgggcgatc agttcagcga
    35881 tttccatcgc cgggctttcg cgcaggtcat caatattcgg tttaaacgcc agaccaaagc
    35941 aggcgatttt cagttcactg gcgcgtttat cggtagcagc aaggcagtcg gcaaccgccg
    36001 ctttcacctg atcgataacc cagaacggtt tgtgatcgtt aacttcgcgc gcggtacgga
    36061 taagccgcgc ctgctggggg ttctgtgcca cgataaacca cggatcaaca gcaatgcagt
    36121 gaccgcccac gccagggcca ggctgaagaa tattaacgcg agggtgacga ttcgccaggc
    36181 gaatcagttc ccagacgtta atcccctgat cggcacaaat cagcgacaat tcattagcaa
    36241 aagcgatatt cacatcgcgg aagctgtttt cggtgagctt acacatttcc gccgtccgcg
    36301 agttagtgac gacacactca ccttcgagga aaattttgta cagttcgctg gcgcgggtcg
    36361 aacaaaccgg cgtcatacca ccaatcacgc gatcgttttt aatcagctcg accataacct
    36421 ggcccggtaa cacgcgttcc gggcagtaag caatgttgac gtccgcctgc tcgcccacct
    36481 gctgtgggaa agtgagatcc ggacgcatct ctgctaacca ttctgccatc ttctcggttg
    36541 accccaccgg cgaggtggat tcaaggatca ccagcgcgcc ttttttcagc actggcgcaa
    36601 tggagcgagc agccgattca acgtaggtca tatctggctc atgatcgcct ttaaacggcg
    36661 tgggtacagc aatcagccag gcatccgctt caactggcgt cgtgctcgct cgtaaaaaac
    36721 cgccttctac ggcagttttt actacgctcg ccaaatcagg ctcgacgata tggatttcgc
    36781 cacgattgat ggtatcaacc gcgtgttggt tgatatcgac accaatcacc tgtttttgcc
    36841 gtgaggcaaa cgctgctgct gtaggcagcc cgatgtaacc cagtccgata acagaaatgg
    36901 tcgcaaaact catagtgata tccgattatt ttttaacgct tccagaatgc gagagcatgc
    36961 ctgaccatca ccatacgggt tatgggcgcg gctcatagtt tgatattcgt tttcgtcttt
    37021 taaaagacgc gtcacttcct cgacaattcg ctgcttatcc gtgcctacca gacgcaccgt
    37081 acccgccgtc accgcttccg gacgctcagt ggtatcgcgc atcaccagca ccggtttccc
    37141 cagcgaaggc gcttcttcct gaatgccgcc tgagtcggtc aaaatcagcc aggcgtggtt
    37201 catcagccag acaaacggta aatactcctg gggatcgatc agaatgacat ttttcacatg
    37261 ccccagaatg cgattgaccg gctctctgac gttcgggttg agatgcaccg gatagacaat
    37321 ctggatgtcc tggtgcgtgg tggcgatgtc tgccagcgcg tggcagattt cttcaaagcc
    37381 acgaccgaaa ctttcacgcc tgtgaccggt caccagaatc atctttttat cggggtcgat
    37441 aaacgggtaa tttgccgcca gttctgaacg cagcgtgtcg ctgctcatca cctgatcacg
    37501 cacccataac agtgcatcaa tgactgtatt accagtaatg aagattcggc tatccgcaac
    37561 gttttcacgc agcaagtttt gccgggaagt ttcggttgga gagaagtgat acatcgccag
    37621 atgcccggtc aatgtacggt tagcctcttc cggccacggc gaatagagat cgcccgtgcg
    37681 taaaccagcc tcaacgtgac caacaggaat acgctgataa aacgccgcca ggctggttgc
    37741 cagcgtcgtt gttgtgtcgc cgtgaaccag cacgacgtct ggtttgaact cggcaagaat
    37801 aggttttagc ccttccagaa tccgacaggt tatctctgtc aggccctgtc ctggctgcat
    37861 tatgttgaga tcgtagtcag gtacaatgga aaagagtttc agcacctgat cgagcatctc
    37921 ccgatgctgc gcagtgacgc aaactttagc ctcaaaaaaa ggatcttttg ccaacgcatg
    37981 caccaacgga gccatcttga tggcttccgg gcgcgtacca aatacagtca gtactttcac
    38041 atcgattctc ttcgaataag cggcgagcgc ctttgcgctc gccgcagcag tgttgctatt
    38101 tcgagcaacg gcgggttaat gcgacaccag ccccgatcag ccccccgaca atgccccaca
    38161 taatcatcag gaaggcacga cgtgggctat cgcgttttac cggttcttcc ggcgtacgca
    38221 aatagcgata ggtctgaaaa cgcggatcca gagttggacc aacattcagg gtgtttaaca
    38281 tggcccgatt ctgatcatag tcgagatcaa aggccggacc gacggcctgt aaattttcca
    38341 gtcgagcctg gagcattgga cgcccaagca ggaacatttc tgaatcaggt aattcctcgg
    38401 caggcacatc tgtcgcactg cgcgaaatat tatgctgctc agcaattttc agcgcctgct
    38461 caatgctgtt catccggcgg tcgtagatgg ctttcgccac ctcttcctga cgcttcacct
    38521 gagctttcat ctggatggtg cgtgccgccc atgcgccttt cagctcatca ttcagatggc
    38581 tggctgcacg ctggctggca aaagcaacat actgacgtaa caggttatta gcgtcaggcg
    38641 cggtttcggc aataagcttc acgctgtcat tgaccgcgcg ggtaaagtct ccggggataa
    38701 actggatgtt gttaatcatt tcatccagca acgccgcatc ggctttgctg ttgcccacca
    38761 tccgctgttt gtaatagtcg gtttgcagcc agaactctct gcgggtatcc cacgaggcca
    38821 gttgcataac aaactcttta taggcttcgt ccatgaccga tggttgatcg gcagaagcca
    38881 tgtttgaacg gacatccagg ttacgcaaaa attgctgctg cgagtaatat ccccccagca
    38941 tattcaccgt tggacgatcg gtaatcgccg tcgagctcca ctcctgacga gcaaaaaaag
    39001 tatacgccag cgcgattaac gcaaacgcca gccccatgcc aataatccat agcttcccag
    39061 cccacaaggt acgaaacaac ccacgaatat ccagttcatt ttcagcgtct tcggccggtt
    39121 tcccaggcat tggttgtgtc atcacatcct catttatttg gttaaattgg ggctgccacc
    39181 acgatttcta cgcagtctgc gttttacgcg cttaataaag cgagcaactt tccaggcacg
    39241 cttaatgcaa tatccataga ggaagaatgc tagcaaaaag agcaccagca tgacccactc
    39301 cgggacaaaa tgagaatatt ctgccagcac gccaatggaa gcgagcagtg ctgcggcaag
    39361 ggtaatcagc acaaacgcct gacgggaagt aaacccggca cgcatgatca aatggtgaat
    39421 atgctgacgg tcaggagaga atgggctcat gcctttacgc aggcgacggt acataatcgc
    39481 caccatatcc attagcggaa tggcgattat ccacaaagcg gtaaccgggc tgatgggatg
    39541 ggttttgccc tgggtcgttt cgagcaggat ccagataacg gtaaaaccaa tcagcgtact
    39601 gcccgcatca cccataaaga ctttgtagcg gcgacccagg ataccaaggt taagcatgat
    39661 gtatggcagg atggcggcga tcatcgcaaa gcaccagatt gcgaggctgg tttgcccgtc
    39721 gaaccacaaa atcataccga ttgctgcaaa cgagacgcag gacaacccgc ccagcaagcc
    39781 atcaatgcca tcaaccatgt tgaacgcatt aatggccgcc cagacggcaa atagcgtcag
    39841 gaagtaacca aacggtccga gcaccatctc ccaggagcca aagatataac ccaggctact
    39901 gagataaagt ttgccgaaca ccatcataac aatgccaaca gcggcctgta tggtggcacg
    39961 gatttttacg ctgatatcaa aacggtcatc cagcgcgcca atgaaaacaa gcacaccggc
    40021 acaagcgaga tagagagatg catgcggaat atagtaatcg acaattccga acgtgaagca
    40081 aatccctgcg taaaccgaaa tccccccaac gagaggtatc aatccctggt gacgtttgcg
    40141 gaagtttggt ttatccacta aaccgacttt ttttgccacc ttacgggcaa aaaacagaaa
    40201 cagtgtcgtg aataaaaaaa tactgatgag atcagtactc actgtcagta aattcacaat
    40261 gcatgctctc agagaaaatt attagcagaa gtataaccac gaagaccttt attcagaagg
    40321 gaaagtccgt tccgaaccca ccagtcctgt gcaaaaatta accatttgat atgcaaattg
    40381 cataacatgc cagcaaattc caggaatttt cctaacatcg ccagcgcggc attaagatta
    40441 cagatataaa agcaaaacgc cacgtaaaca cgtggcgttt ttggcataag acaaatttat
    40501 gagcgtttca tcatttcgaa gaaatcgtca ttggtcttgg tcatggccag tttattaatg
    40561 aggaattcca ttgcatcgat ttcgcccatc gggtgaatga ttttgcgcag gatccacatt
    40621 ttctgcagtt cttcctgagt cgtgagcagc tcttctttac gggtaccgga acggttgtag
    40681 tcgatagccg ggaagacgcg tttttcagcg atcttacgag agaggtgcag ttccatgttg
    40741 cctgtacctt taaactcttc gtaaataact tcgtccattt tagaaccggt atcgataagc
    40801 gccgtcgcga taatggtcag gctgccgccc tcttccacgt tacgcgccgc accgaagaag
    40861 cgtttcggac gatgaagggc attggcgtcc acaccaccgg tcaacacttt acctgacgcc
    40921 ggaacaacag tgttgtaagc gcgcgccaga cgagtgatgg agtcgagcag aatgataacg
    40981 tctttcttgt gctcaaccag gcgtttggcc ttctcgatca ccatttccgc aacctgaacg
    41041 tggcgagatg cgggttcgtc aaaggtagaa gcaacaactt caccttttac cagacgctgc
    41101 atctcggtta cttcttccgg acgttcgtcg atcagcagaa ccatcagcac acaatccggg
    41161 tggttgtaag caatgctctg agcaatgttc tgcagcagca tagttttacc ggctttcggc
    41221 ggtgccacaa tcagaccacg ctgaccacga ccgataggtg atgccagatc cagtacgcga
    41281 gcggttaaat cttcagtaga accgttacca cgttccatac gcagacgaga gtttgcgtgc
    41341 agcggggtta agttctcaaa gaggattttg ttgcgggcgt tttcaggttt gtcgaagtta
    41401 acttcgttaa ctttcagcag cgcaaaatag cgttcacctt ctttcggcgg gcgaatctta
    41461 ccagagatgg tatcaccagt gcggaggttg aaacggcgga tttggctagg ggaaacgtag
    41521 atgtcatcag gaccggcgag gtaggagctg tctgcggaac ggaggaaacc aaatccatcc
    41581 tgcaatatct ccagtacgcc atcaccaaag atatcttcgc cactctttgc gtgctgcttc
    41641 aggatggcaa aaataatgtc ctgcttacgc atacgagcca ggttttccag ccccatattt
    41701 tcgccgagag tgatcagctc agaaaccggc gtattcttta attcggtaag attcatagtg
    41761 gtgtgagttc ttaaacttgg ggtaaatctc gaacttaatg ttgtgaatgg tatggcagga
    41821 tcatccatgc ctgtttacgg tcatcgactc atgtcttttc gctgcctggt cacaggagag
    41881 tacgcagaac tgaaacgaca agacggattg agtgacgagc cagaaatctg ttcacttcgc
    41941 atttaagata aaacgggaag cgttggaaat tacaagattc aaacttaata aggtatgttt
    42001 aatacgaagt caacactaag ttagcatgac tcacgccggg cgtccagttt ttagcgacgg
    42061 ggcacccgaa catgaaattc ccttacgcca ggttagcgtc gaggaactct ttcaactgac
    42121 ctttagacag tgcacccact ttggttgccg ccacttcacc gtttttgaac agcagcagag
    42181 tcgggatacc acggatgcca tatttcggcg cagtgccagg gttttgatcg atgttcagtt
    42241 ttgcaacggt cagtttgccc tgatattcgt cagcgatttc atccagaatc ggggcgatca
    42301 ttttgcacgg accgcaccac tctgcccaga aatcgacgag gatcgccccg tccgctttga
    42361 gtacatccgt gtcaaaactg tcgtcagtca ggtgaataat tttatcgctc atatataact
    42421 ccacaggaat aagcctggcg tgttggtttc gttgttggtg taacattaac caactaaagg
    42481 ttgactttat ttcaccggat acgctttcgt aaagcaatag taagctgata ttctaccaca
    42541 ctatgagcaa aacacattta acagaacaga agttttccga cttcgccctg catccgaagg
    42601 ttgtagaagc ccttgaaaaa aaagggtttc ataactgtac acccattcag gcactggccc
    42661 ttccgctgac gctggcgggt cgtgacgtag ccgggcaggc gcaaaccggt accgggaaaa
    42721 cgatggcgtt tcttacgtca acgtttcatt atcttctctc tcatcctgcg attgccgatc
    42781 gcaaggtgaa tcaaccacgt gccttaatta tggcaccgac gcgtgaactt gccgtgcaga
    42841 tccatgccga cgcagaaccg ctggcggaag ctaccggcct gaagctgggt ctggcttacg
    42901 gtggtgatgg ctacgacaaa cagctgaaag tgctggaaag cggcgttgac attctgattg
    42961 gcactacggg gcgtttaatt gactacgcca agcagaacca cattaacctc ggtgccattc
    43021 aggtggtggt actggacgaa gccgatcgca tgtacgatct gggctttatt aaagatatcc
    43081 gctggctgtt ccgccgtatg ccgcctgcaa accagcgcct caacatgctg ttctccgcca
    43141 cgctttcgta ccgggtacgt gaactggcgt tcgagcagat gaacaatgcc gaatatattg
    43201 aagtggaacc ggaacagaaa acgggccacc gtattaaaga agagcttttc tacccttcta
    43261 acgaagaaaa aatgcgtttg ctgcaaacgc tgatcgaaga agagtggcca gacagagcga
    43321 ttattttcgc caataccaaa caccgttgtg aagagatctg gggccacctg gcggcagatg
    43381 gtcatcgtgt cggtttgttg acaggcgatg tcgcgcagaa aaaacgtctg cgtattcttg
    43441 atgaatttac ccgtggcgat ctggatattc tggttgccac cgacgttgcc gcgcgtggtt
    43501 tgcatattcc ggcagtgacg cacgtcttta actacgattt acccgatgac tgtgaagatt
    43561 acgttcaccg tattggtcgt acaggtcgcg caggcgcaag cggtcactct atcagcctgg
    43621 cgtgtgaaga gtatgcattg aatttgcctg ctattgagac ctatattggt cactcaattc
    43681 cggtaagcaa atacaatccg gacgcattga tgaccgatct gccaaaaccg ctgcgcctca
    43741 cgcgcccacg tacaggcaat ggtccgcgtc gtactggcgc tccgcgtaat cgtcgtcgtt
    43801 caggttaaat aaaatgtcat actcaaaatc attcaaactg tatcaagatg gcaagagaat
    43861 gaatcccgat gagcatactg tagtaagtga ttcaggtgaa agaatgcagc caacacagag
    43921 acagattgaa ggatgaagag tatgggttcc acctcgtcgc tgtatgcagc cattgatctc
    43981 ggttcgaata gttttcatat gctggttgtg cgcgaggtgg ctggaagcat ccagacgctg
    44041 acgcgaataa aacgcaaagt gcgtctggct gctggcctga atagcgaaaa tgccctgtct
    44101 aatgaagcaa tggagcgcgg ttggcaatgt ctgcgcctgt ttgctgaacg tctgcaagat
    44161 atccctccct cgcaaattcg cgttgtcgct acggcgacat tacgtcttgc cgtcaatgcg
    44221 ggtgatttta ttgccaaagc gcaggaaatc ctcggttgtc cggtacaggt gatcagcggt
    44281 gaagaggaag cacgtctgat ttatcagggc gttgctcaca caactggcgg tgccgatcag
    44341 cgcctggtgg tggatatagg cggtgccagt actgaactgg taaccggcac gggtgcacaa
    44401 accacctcgt tgttcagcct gtcgatgggc tgcgtcacct ggctggaacg ctattttgcc
    44461 gatcgtaatc tggggcagga aaattttgat gctgcagaaa aagcggcacg cgaagtgtta
    44521 cgtccggttg ccgatgaatt acggtatcac ggctggaaag tgtgcgttgg tgcttccggc
    44581 accgtgcagg cgttacagga aatcatgatg gcacagggga tggatgaacg cattaccctg
    44641 gaaaagttgc agcaattgaa acagcgagcc attcattgcg gtcggctgga ggaactggag
    44701 attgacgggc tgacgctgga acgtgcgtta gtgttcccga gtggtctggc gatcctgatc
    44761 gccattttta ccgaactgaa tattcagtgt atgaccctgg cgggcggtgc gctgcgtgaa
    44821 ggcctggtct acggcatgtt gcatctggcc gtcgagcagg atattcgcag ccgtacgctg
    44881 cgtaatattc agcgccgctt tatgatcgat attgatcagg cgcagcgcgt agccaaagtt
    44941 gcggctaact tcttcgatca ggtggaaaag gaatggcatc ttgaagcaat aagccgcgat
    45001 ttgctcatca gcgcctgtca gcttcatgaa atcggcctga gcgttgactt taaacaagcg
    45061 ccgcaacacg ctgcttatct ggtgcgtaat ctggatcttc ccggttttac ccccgcacag
    45121 aaaaaattgc tggcgacgct actgctcaac cagactaatc cggtcgatct ctcatcgctg
    45181 catcagcaaa atgccgtacc gccgcgcgtc gcagaacaac tctgccgttt actccgcctg
    45241 gccatcattt ttgccagccg tcgccgtgac gatctcgtgc cagagatgac attacaggct
    45301 aaccatgaac tgttgacctt gacgcttccg caaggttggc taacccaaca tccgctgggt
    45361 aaagagatta ttgctcagga aagccagtgg cagagctatg tccactggcc gctggaagtg
    45421 cattaatgag taagtgccgg atgcgatgct gacgcatctt ttccggcctt gattatttcc
    45481 ctcgttttgc cgccatcatc gctttcagat tcgccagatg gctttgccct ttctgcatcc
    45541 gttcttcggc gctgaccact ttgcgctcct gttcccaaat cagatcatcc tgcggcagct
    45601 ccagcaaaaa gcggctcggc tccgggcgca ccagctcgcc gtactgacgg cgttctttac
    45661 acagcgtaaa ggtcaattcc ttctgggcgc gggtaatgcc gacataggcc agccgccgct
    45721 cctcatcgat attatcttca tcgatgctgc tctggtgcgg caaaaaccct tcttccatac
    45781 cgaccatgta gacataagga aactccagcc cttttgacgc gtgcagagtc atcagttgca
    45841 cctgatccag ctcttcttca ctctcaccac gctccatcat gtcgcgcaaa gtaaagcgcg
    45901 tcaccacctg ggtgagcgtc atcggctcat ccagttcgct gccttccagc atctccgtca
    45961 tccagctaaa cagttggttg acgttcttca tgcgcatttc ggcggctttc gggctgggcg
    46021 atgtttcgta cagccaggat tcataatcca tgccgtggat cagatcgcgc accgcggcaa
    46081 tcggctcccg ctccgccaga cgctggattt ctgccaacca gtgagtaaag cgggtcaatg
    46141 cttcataacc acgtccgcta agcgtctgac tcaggcccat atcaaagctg gcggtaaaca
    46201 tgcttttatt gcgcgtcatc gcccactcac ccagcttttt cagcgtcgcc gggccaatct
    46261 ctctcttcgg cgtgttaacg atacgcagaa atgcgctgtc atcgtccggg ttagtcagca
    46321 cgcgcagata agccagcaag tccttgattt caggacgaga gaaaaacgac gtaccaccag
    46381 atattttgta tgggatgcgg ttttgcatca ggaatttttc aaacacccgc gactgatggt
    46441 taccgcgata aagaatggcg taatctttgt actgcgtttt attgacgaag tgatgggcga
    46501 tcagctcgcc agtaacgcgc tcggcttcat gttcctcgtt attcgcgctt aaaactttta
    46561 gctctgtgcc gtaacccagt tcggagaaca gacgcttttc aaagacgtgc ggattattgg
    46621 cgatcaggat gttcgccgct ttcaaaatac gtccggaaga gcggtagttc tgctcaagct
    46681 taatcacctt cagcgccgga aaatcctgac tcagcagcac caggttttgt ggacgtgcgc
    46741 cgcgccagga gtagatcgac tggtcgtcat cgcccaccac ggtaaagcgc gcgcggctgc
    46801 ccaccagcag tttcaccagt tcatactggc tggtgttggt gtcctgatac tcatccacca
    46861 gcagatagcg aattttgttc tgccagcgct cgcggacttc ttcattgcgt tgcagcagca
    46921 acgtcggcaa taaaatcaga tcatcgaagt cgagaacatt gcacgctttc aggtgtgcat
    46981 catacagccc ataacaatgg gcaaaaatac ggtcccgctc gccaatcgca ctggctgccg
    47041 cctgggacgg tgttttgaga tcgttcttcc agttagagat ggtcgaaatc aactgttgca
    47101 ggagaacttt gtcatcttca atcagccctt cggtcaactc tttgagcaac gccagctgat
    47161 cggtatcgtc aaacagcgaa aagttcgcct tcatccccag cgccgcatac tcacgcttga
    47221 tgatatccag ccccagcgtg tggaaggttg agatcatcag cccacgcgcc tctttgcgac
    47281 ccagcgtctg tccgacacgc tctttcatct cgcgcgccgc cttattggta aaggtcaccg
    47341 ccgcgatatg tcgcgcctga taaccgcaac cgcggatcag atgggcgatt ttattggtga
    47401 tcacacgagt tttaccggaa cccgcgcccg ccagcaccag gcaggggccg gtaacgaatt
    47461 cgacagcttg ttgttggccg gggtttagac gcataggtgt attgctcaat cttcgaacgg
    47521 ggggaggatt gtagcagaat cgcatttgaa tgtgaccgcg tgtaagccgc taaccggttg
    47581 gctgcggatg tttaaatcag agagagaaga ccgaaaccgg agttcatcgt gaatccatta
    47641 gacttagaaa atatcgggtc gtaacgttgt gttattactt tttcagagtt tactaaggag
    47701 aggtgtactc gtggcgatta atttcagtcc taaggttggt gaaatactgg aatgcaactt
    47761 tggtaactat cctgttagcc agaatggaca gttcagcacc aagtattacg atggccgcat
    47821 tcctcctgaa atgataaaaa acaggttagt tgtcgtgctc aacggaaaaa ttaatggcaa
    47881 cgcatgtatc gtcgttccat tatcaaccac tcgcgatcat gacaagttaa atcgtggaat
    47941 gcatgttgaa atagccagta atgttatcaa tggcctgcaa ttctttgatc agcagattcg
    48001 ttgggccaaa gccgacctgg tacaacaagt cagtcgaaac cgattaaata gagcaaggac
    48061 ttacagaggg tatcttaatc aatgtttgcc acatgagttg gttgcagaca tacagagggc
    48121 ggtcatcaag tcaatcaatg ccatatcgct cataaattga caataaaata accatccccg
    48181 ataatgactt tgtagcccat agaggcccta agttttctcc ccttgaggga cctgcgaagc
    48241 ccggattttc cgggcttttg ccatatatgt accagcccct ccctgactga caattacgtt
    48301 agcatctgcg gatcatgcta aaatcgccgc ctgacaatta ttcactcaag gcacgatcat
    48361 ggcaaaaaca gcagcagcac tgcatatcct tgtaaaagaa gagaaactgg ctctggatct
    48421 tctcgagcag attaagaacg gtgccgattt cggcaagctg gcgaagaaac actccatttg
    48481 cccatcaggc aaacgcggcg gtgatttagg taaattccgc cagggtcaga tggttccggc
    48541 gttcgataaa gttgtgttct cttgtccggt actggagccg accggcccgc tgcacaccca
    48601 gttcggttac cacatcatta aggtgctgta ccgcaactaa tagcaaggcc ttctccagga
    48661 gaaggccttg agtgttttct ccctctccct gtgggagagg gtcggggtga gggcatcagc
    48721 gcgcacttaa cccgcaacag caatacgttt catatctgtc atatagccgc gcagtttctt
    48781 acctacctgc tcaatcgcat ggctgcgaat cgcttcgttc acatcacgca gttgcgcgtt
    48841 atctaccgcg ccttccggaa tagctttacc caagtcgccc ggttgcagct ctgccataaa
    48901 cggtttcagc aacggcacac aagcgtaaga gaacagatag ttaccgtact cagcggtatc
    48961 agagataacc acgttcattt cgtacagacg cttacgggcg atggtgttgg caatcagcgg
    49021 cagctcgtgc agtgattcat aatatgcaga ctcttcaatg atgccggaat cgaccatggt
    49081 ttcgaacgcc agttcaacgc ccgctttcac catcgcgatc atcagtacgc ctttatcgaa
    49141 gtactcctgc tcgccgattt tgccttcata ctgcggcgcg gtttcaaacg cggttttgcc
    49201 ggtctcttca cgccaggtca gcagtttctt atcatcgttg gcccagtccg ccatcatacc
    49261 ggaagagaat tcgccggaga tgatgtcgtc catatgtttc tggaacagcg gtgccatgat
    49321 ctctttcagc tgttcagaaa gcgcgtaagc acgcagtttc gccgggttag agagacggtc
    49381 catcatcagg gtgatgccgc cctgcttcag cgcttcggtg atggtttccc aaccgaactg
    49441 aattagtttt tctgcgtatg ccggatcggt gccctcttcc accagcttgt cgaagcacag
    49501 cagcgagcca gcctgcaaca taccgcacag gatggtttgc tcacccatca ggtcagattt
    49561 cacttccgca acgaaggacg attccagcac gcccgcacgg tgaccaccgg ttgctgccgc
    49621 ccatgcttta gcgatcgcca taccttcgcc tttcggatcg ttttccgggt gaacggcaat
    49681 cagcgtcggt acgccgaatc cacgtttgta ctcttcacgc acttcggtgc cagggcattt
    49741 cggcgcaacc atgactacgg tgatatcttt acggatctgc tcgcccactt cgacgatgtt
    49801 gaaaccgtgc gagtaaccca gcgccgcgcc gtctttcatc agtggctgta cggtgcgcac
    49861 tacatcagag tgctgcttgt ccggcgtcag gttaaccacc agatccgcct gcgggatcag
    49921 ttcttcgtaa gtaccaactt taaaaccgtt ttcggtcgct ttacgccagg acgcgcgctt
    49981 ctcggcaatc gcttctttac gcagagcgta agaaatatcc agaccagaat cacgcatgtt
    50041 caggccctgg ttcagaccct gtgcgccaca gccgacgatg actacttttt taccctgaag
    50101 gtagctcgcg ccatcggcga attcatcgcg ccccataaag cgacatttgc ccagctgtgc
    50161 cagctgctga cgcagattca gtgtattgaa gtagttagcc atggtgattc ctcgtgatgt
    50221 tgtgcttctt attgttcggt tcgcgtttgc gagattgaat tcactatatg acaggaaatt
    50281 tattgcggaa attgatatat tcacaacgtc acattgcaat ttttgcaacg tcaacatcga
    50341 gggctgcgcc attggattta cgcgatctga aaaccttcct gcatctggcg gaaagccgcc
    50401 attttggccg cagcgcgcgg gcgatgcacg ttagcccatc cacgctctca cggcagattc
    50461 agcgcctgga agaagatctt ggtcagccgc tatttgtgcg cgataaccgc acggtaacgc
    50521 tgacggaagc gggcgaagag ctgcgcgttt tcgcccagca aacgctgttg cagtatcagc
    50581 aattgcgcca caccatcgat cagcaagggc cgtcgctctc tggcgaatta catatcttct
    50641 gctcggtgac cgctgcctac agccatctgc cgccgattct ggatcgcttc cgcgcggaac
    50701 acccgtcggt ggagatcaaa ctcaccactg gtgatgcggc agatgcaatg gaaaaagtgg
    50761 tcacgggaga agcggatctg gcgattgccg ggaaaccgga gacgctaccc ggcgctgtgg
    50821 cgttttcgat gctggagaat ctggcagtag tgctgattgc ccccgcgctg ccctgcccgg
    50881 tgcgtaatca ggtatctgtc gaaaagcccg actggtcaac ggtgccgttt attatggccg
    50941 atcaggggcc ggtacgccgc cgcattgaac tgtggtttcg acgcaataaa atcagcaacc
    51001 cgatgattta cgccacggtt ggcgggcatg aagcgatggt atcgatggtg gcgctcggct
    51061 gtggtgtggc gctgttgccg gaagtggtgc tggaaaacag cccggagcct gtacgcaacc
    51121 gggtaatgat tttagatcgc agcgatgaga aaacgccgtt tgaactgggc gtgtgtgcac
    51181 aaaaaaagcg gctgcatgag ccgctgattg aggcgttctg gaggattttg ccgaaccaca
    51241 aatgacgttg tcgcgcgggt aggcctgata agcgaagcgc tatcaggcat ttttccctaa
    51301 cccgccaaaa agaacctgaa cgccggatta ttggtttcgt cgtggcaatc gtagcccagc
    51361 tcatttagcc gggtttcgaa atccggttca tggtcgccga gttcgaacgc cgccagtacg
    51421 cgcccgtagt cggtgccatg gctgcgatag tggaacaaag aaatgttcca gtgcgtaccc
    51481 agcgtgttga ggaagcgcag cagcgcgccc ggtgattccg ggaattcgaa gctgtagagg
    51541 cgttcctgca acggatgtga cggacgcccg ccgaccatat agcgcacatg caacttcgcc
    51601 atttcgtcgt cggagagatc caccacgctg tagccgccgt cgttgagcat ctgcaaaatt
    51661 tctttgcgct cttcgaggcc gcggctcagg cgcacgccga caaagatgca ggcgtttttg
    51721 gcatcggcaa aacggtagtt gaactcggtg accgatcgcc cgccaagcag ttggcagaat
    51781 ttgaggaagc tgcctttttc ttccggaatg gtcaccgcca acaacgcttc acgctgttcg
    51841 cccagttcgc agcgttccga gacgtagcgc aggccgtgga agttcacgtt ggcaccggaa
    51901 agaatatgcg ccagccgttc gccgcgaatg ttgtgctggg cgatatattt tttcattccc
    51961 gccagcgcca gcgcgccaga gggttccgcc accgcgcgca catcttcgaa taaatccttc
    52021 atcgccgcac agatcgcatc actatcgacg gtgatgatgt cgtcgagata ctcctggcat
    52081 aaacggaagg tttcgtcacc gatgcgtttt accgctacgc cttcagcaaa tagccctacg
    52141 cgcggcagat caaccggatg acccgcatcc agcgctgctt tcaggcaggc ggagtcttcc
    52201 gcttctacgg cgatcacttt gatttgtggc atcagttgtt tgatcagcac cgcaacgccc
    52261 gccgccagac cgccgccgcc gactggcaca aatacgcggt cgagatgggc gtcctgctgg
    52321 agcagttcca gcgccagcgt gccttgcccg gcaatcacca tcggatggtc gaacggcggc
    52381 acccaggtga acccctgctg ctgtgacagt tcgatcgctt tagctttcgc ttcgtcaaag
    52441 ttcgcgccat gaagcagcac ttcgccgccg aatccacgta ccgcatcgac tttgatgtcg
    52501 gcggtggcgg ttggcataac gatcagggcc ttcacgccta accgcgcaga agaaaacgcg
    52561 acgccctgcg cgtggttacc cgcagaagca gtgatcacgc cgtgcgcttt ttgttcttcc
    52621 gtcaggcccg ccatcatggc gtatgcgccg cgcagcttaa agctgtgcac tggctggcga
    52681 tcttcgcgct tcaccagaat gacgttatca agacgcgacg acagtttttc cattttttgt
    52741 agcggcgtaa cctgcgccgc ctcgtaaacc ggcgcgcgca gcactgctct taaatattcg
    52801 gcaccttccg gagcaccgga caggggttgc gagtcagcca ttattaaccc cccagtttcg
    52861 atttatcgcg caccgcacct ttgtcggcgc tggttgccag gctggcgtag gcgcgcaggg
    52921 caaaggaaac ctgacgttca cggtttttcg gcgtccaggc tttgtcaccc cgagcttcct
    52981 gcgcttcacg acgcgccgcc agttcggcat cgcttacctg taactgaatg ccacggttcg
    53041 ggatgtcgat agcgatcagg tcaccatctt caatcaggcc aatgctgccg ccgcttgccg
    53101 cttccggtga gacgtggccg atggaaagac cagaggtgcc gccagagaaa cgaccgtcgg
    53161 tgatcagcgc acaggcttta ccgagaccca ttgatttcag gaagctggtt gggtagagca
    53221 tttcctgcat gccagggccg cctttcgggc cttcatagcg gatcaccacc acatcgcccg
    53281 cgacaacttt gccaccgagg atcgcttcta ccgcgtcgtc ctggctttcg tacactttcg
    53341 ccgggccggt gaatttgagg atgctgtcat cgacgcccgc ggttttaacg atgcagccgt
    53401 tttctgcgaa attaccgtag agcaccgcca ggccgccgtc tttgctgtag gcgtgttcca
    53461 gcgagcggat acagccattg gcgcgatcgt catcgagaga atcccaacgg caatcctgcg
    53521 agaatgcctg tgtggtacga atgcctgcag gacctgcgcg gaacatattt tttaccgcgt
    53581 catcctgggt cagcataacg tcgtattgtt ccagcgtttg cggcaacgtc aggccaagta
    53641 cgtttttcac atcacggttc agtaaccccg cgcgatccag ttcgccgaga ataccgataa
    53701 caccaccagc acggtgaaca tcttccatat ggtatttctg ggtgctcggc gcaactttac
    53761 acagctgtgg aaccttgcgg gaaagcttat cgatatcact catggtgaag tcgatttccg
    53821 cttcctgcgc cgccgccagc aggtgaagta cggtgttagt cgatccaccc atcgcgatat
    53881 ccagcgtcat ggcgttttca aacgccgctt tgctagcaat attgcgcggc agtgcacttt
    53941 cgtcgttttg ctcgtagtaa cgtttggtca attcaacaat gcgtttaccg gcattgagga
    54001 acaactgctt acggtccgcg tgggttgcca gcagcgagcc gttgcccggc tgcgacaggc
    54061 ccagcgcttc ggtcaggcag ttcattgagt tagcggtaaa catcccggag caggagccgc
    54121 aggtcggaca cgcggaacgt tcaacctgat cgctctggga gtcagaaact ttcgggtctg
    54181 cgccctggat catcgcatca accagatcga gtttgattat ctgatcggac agtttggttt
    54241 tcccggcctc catcgggccg ccggaaacaa agatcaccgg aatattcagg cgcagggaag
    54301 ccatcagcat ccccggggtg attttgtcgc agttagagat gcagaccatg gcgtcggcgc
    54361 agtgggcgtt gaccatatac tcaacggaat cagcgatcag ttcgcgagat ggaagtgaat
    54421 aaagcatccc cccgtggccc atggcaatcc catcatccac cgcaatggtg ttgaactctt
    54481 tggcaacgcc gccagccgct tcaatttgtt cggcgaccag tttaccgaga tcgcgcagat
    54541 ggacgtgacc cggtacaaat tgggtgaacg agttcacaac cgcgataatc ggcttgccaa
    54601 aatcggcgtc ggtcattccg gtggcgcgcc acagcgcacg agcacccgcc atattacgac
    54661 catgagtggt ggtggcggaa cggtacttag gcatacttta tttactccca gtgtctgtct
    54721 cgtaaatggg acggtgcgtg ccgtcccatt ttttgtattt attgattaac ttgatctaac
    54781 cagccccatt tatcttcggt ttcgccagtg aagaggccga agaaggcttg ctgaatgcgt
    54841 ttggtaaccg ggccacaacg gccttcgcca acctgaatac catctacact gcgcactggc
    54901 gtgatttctg ccgcagtacc ggacataaac acttcatccg ccaggtacag ggattcgcgc
    54961 gacagaacct gctcacgcac ttcaattccc agctctttcg ccagtttgat gatggcatca
    55021 cgggtaatac ccggcagcgc ggaggaggtg aacggtgggg tgaacagcac accatctttc
    55081 acttcaaaca ggttttcgcc tgcgccttca gagatataac cgttcacatc cagcgcgata
    55141 ccttcctgat aaccgtggcg gcgcgcttcg ctacccacca gcagggaaga gagatagtta
    55201 ccaccggctt ttgccgcggt tgggatggtg tttggtgctg cgcggttcca ggaggaaacc
    55261 atcgcatcga tcccctgctc cagcgcttct gcgcccagat acgctcccca cgggaaagcg
    55321 gcgataatca catcggttga gtatcccgct ggcgggttta cacccatgcc aacatcacca
    55381 acgaagatca gcggacggat ataggcgctg gtgagattgt ttttgcggat cacgtcacga
    55441 caagcttcca tcagctcatc aatgctctgc gaaaccggga agcgatagat tttggcggag
    55501 tcatgcagac gctgcatatg ctcacgatgg cggaatacaa ccggtccttt gtgcgagtcg
    55561 tagcaacgga tgccttcaaa aaccgaggtg ccatagtgca gcgcgtgcga catcacatgc
    55621 accttcgcgt cttcccagcg aaccatctcc ccattgaacc aaatgtaatc agctttcttc
    55681 gtggtcattt ttatattcct tttgcgctca ggcgcggatt tgttgtgatg tggttgtgct
    55741 ctggcagatg gcaacgtgtg cgacgtccac cagtttattt aactgactaa acagtaagtc
    55801 gaccgaccgt gggctggcaa cggtcaattc gatatttata ttttgtgcat cgctggcggc
    55861 ggccatattc attgagcaga cgtggaaacc acgatgacgc accacgcgta aaacacgttc
    55921 taaggtttct ggattgaagc gagccgatac attgacctga tgttgcatca tgataatttc
    55981 tccaacattt ctgaattact ggcgccaggc ggcaccagcg gccagacgtt ctcaagttcg
    56041 tcgattgaga catgaagcag gtatggccca tcactgttca gcatggtgtc gagtgccgct
    56101 tcaacctggt ctttacgggt gatgtgttgg ccagggatgc cgaaggcgct ggctaacatg
    56161 aggaaatcgg ggttatcagt aagggtggtt tcgctgtatc gttcctgaaa aaacagttgc
    56221 tgccattgtc gaaccatccc taaccgttgg ttatcgagta agacgatttt caacggtaac
    56281 tgcttgcgtt ttacggtgcc cagctcttgc acattcatca tgaaagagcc gtcaccggag
    56341 atacagacaa cggtatcgtt cggtcgcgcg acttgtgcgc caaccgccgc cggtaaacca
    56401 aaacccatgg tacctaagcc gctggaggtg atgaaatttt ccgggcgagt gtgggcgatg
    56461 tgctgcgcag cccacatctg gtgctgcccc acatctgtgg tcacgacgca atccgcaggt
    56521 ttacgatccg acagttgttt taacaacaac ggcgcgtaga tagcgtcacc gggatggtcg
    56581 taacgccagg aatgttcatc acgcagctgc gcgcagtgtt gctgccagtc attgatattt
    56641 aacggctgct gtaatgctgg taacagagca tttaaatcac cttgtaatgc cacatgtgcc
    56701 tgacgcagct tgttcatttc tgccgggtcg atatccatat ggataacact ggcgtgtggc
    56761 gcgaaggtgt tcagtttgcc ggtcacccgg tcatcaaaac gtgcacccac ggcgatcagc
    56821 aagtcgcact cctgcaccgc gaagttcgcc gctttggtgc cgtgcattcc cagcatgccc
    56881 agatagtacg gataatctgc ttcaactgcg cccagccctt tcagcgtgca ggtggcaggc
    56941 atttttgtgg tagcgagaaa ttctcgtaaa gcaggaactg cctgcgccat acccacgcca
    57001 ccaccaacgt acagcatcgg tttttgcgct tttgccagca tctggcgcgc ttgctcaact
    57061 tcggcatggg ggaaagtcac ttcgttttca acggtggtga accacggttc cagatcgccg
    57121 ctggctaact ggatatcttt tgggatatcg accagaaccg gaccaggacg acctgagctg
    57181 gcaacgtcga atgcttcagc catgatgcgc ggcaactctt ccagcgactg caccagaaag
    57241 ctgtgcttgg tacaggctaa cgacaatccc aggacatcca cttcctgaaa tgcgtcggtg
    57301 ccgataaacg gtgcggacac ctgaccagtg atggcaacga cagggatgga atctaacagt
    57361 gcgtccgcaa gcccggttat caggttggtt gcgcccggac cagacgtggc gatacatacg
    57421 ccagttttac cggtagcacg ggcataaccg atagccgcca ttgccgcacc ctgctcatgt
    57481 cggcacagca agtgctccac gccgccgtca tacaatgcat cgtaaaccgg cataattgcg
    57541 ccacccggat aaccgaaaac ggtgttcaca ccctgtgccc gcaacgcatg taccacccac
    57601 tgtgcgccat tcatagttag ttccccgtcc tgaatcttga gaaacagaat tttgtgctgt
    57661 tattcattgt ctgctcctcg gttatgtttt taaggtcaaa aaaaaccccc ggacctttcg
    57721 gtgcgggggt cttagttcgt taaggcttga tctctaagcc tttcctcgtc caagtgcagc
    57781 cccgcacggt gggataataa tcaccaccac gctaatcacg accaggctaa tcactcgtag
    57841 aagggctgtc attttgtctt ttcttgcatc ttgttcgaag gaatgcctaa agagttacca
    57901 taggttttgt aaatagtaca agatattttt tggccgattt ttatgatgcg tttaataatt
    57961 tcaatataaa ttgttgtttt ttaaaaataa aatagtgagt gaaaaaaatt caatttcagt
    58021 atgaatgttg ttcccttcct cgtagttcat cctttgtccg ctgcattttt gcgagcatca
    58081 ttccaccgcc agaacaactc aacttcactt gcacaaataa acaggaatca gccactaaaa
    58141 ttcacgaatt agcgggctac agcagcgata tcttatgcac ataatggtga ctcaaggagg
    58201 gcttatgtca ctgtcaattg ttcatacccg cgcagccctg ggagtaaatg cgcccccgat
    58261 cactgttgag gtacatatca gtaaaggtct acccggctta acgatggtgg gcttaccaga
    58321 aacaacggta aaagaagctc gcgatcgcgt gcgcagcgcc attatcaata gcggatatga
    58381 atatccggcg aaaaaaatca ccatcaacct ggctccagct gatctgccaa aagaaggggg
    58441 acgatatgat ttacctatcg ccattgcgtt gctggcggcc tcagaacagc ttacagccaa
    58501 taagttagat gaatatgaat tagtcggaga actggcgctt acaggcgctc tgcgtggcgt
    58561 tcccggcgca atctccagtg caactgaagc tattaagtcg ggcagaaaaa ttatcgtcgc
    58621 gaaagataac gaagatgaag tggggctaat taacggtgaa ggatgcctga tagccgatca
    58681 tctgcaagct gtctgtgcgt ttctggaagg taagcacgct ctcgaacgcc cgaaaccaac
    58741 tgatgcagta tcccgggcgc tacaacatga tctcagtgat gttgtcggtc aggaacaagg
    58801 aaagcgagga ctggaaatta ccgccgctgg cgggcacaac cttttactga ttgggccgcc
    58861 gggaacaggt aaaacaatgc tcgccagccg tattaatggc ctgttgccag atttaagcaa
    58921 tgaagaggca ctggagagtg ctgcgatatt aagtctggta aatgctgaat cagtacaaaa
    58981 acaatggcgg cagcgcccgt tccgctcacc tcatcacagt gcatcgttaa ctgcgatggt
    59041 aggcggtggc gcaattccag ggcccggtga aatttcgctg gcgcataacg gcgtgctttt
    59101 tcttgatgag ttacctgaat ttgaaaggcg tacactggat gccttgcgag agccgattga
    59161 atccgggcag atccatcttt cacgcacgcg agcaaaaata acctatccag cccgtttcca
    59221 gcttgtcgcg gcgatgaatc ccagccctac cggacattat cagggaaacc ataaccgctg
    59281 cacaccagag caaacgctgc gttatctcaa ccgactctct ggtccctttc tcgaccgctt
    59341 cgatctctca ctggagatcc cattaccacc ccccggcatt ttgagtaaaa cggtagtgcc
    59401 gggagaaagc agcgccaccg ttaaacaacg cgtaatggcc gccagagagc gccaatttaa
    59461 gcggcagaat aaactgaatg cctggctgga tagtccggaa atacgccaat tctgcaaact
    59521 tgagagcgaa gatgcgatgt ggttggaagg aacactgatc catctggggt tatcgattcg
    59581 tgcctggcag cggttattga aagttgcacg aaccattgct gatattgatc agtctgacat
    59641 tatcacacgt cagcatttgc aggaggcagt tagctatcgt gcgattgacc gtttgctcat
    59701 ccatctgcaa aaactactga cataaaaaaa gggcatttcg ccctttttat taatcatcag
    59761 aatcggtgta gtcttcagca ccttcaacct gcggtttacc gccggaaagg gtgtgaaaac
    59821 gttttggacg cttaatacgc gtcatatact tggaccacac gcgttctgct tctgtcactg
    59881 gctcacgttc gccacggcat actgctacga agagtttctc ttcctcggta accggctcgc
    59941 gtttgccaag atccaactca ttgaaggcat aaccatgacg ctcaagcagt tgtgcctctt
    60001 tgatggtgaa atcaccatga cgagagaatc cacgtggata atgtttattg tcgaaatatc
    60061 gattagtcgt cgtaaagctt tccgccatcc tgcacgctcc taattctttg accgagctag
    60121 ttatggcgcg gagtattagt tacgcttgac agagtgtaaa acaaaacatt taaatcataa
    60181 cgacaaataa ttttgcggag agcactgtgg atacggaatt gttaaaaact ttcctggaag
    60241 ttagccgaac gcgtcacttt ggtcgagcgg ctgaatcgct ctatctgacc cagtcagcag
    60301 tgagctttcg aatcagacaa ctggaaaatc aactgggtgt gaaccttttc acccgccaca
    60361 gaaacaatat ccgtttaacc gctgccggtg aaaaactact gccttatgca gaaacgctca
    60421 tgagcacctg gcaggccgcc cgtaaggagg tggcgcatac ctcacgacat aacgagtttt
    60481 ctatcggtgc cagcgcctcg ttgtgggaat gtatgcttaa tcagtggctg ggacgtttgt
    60541 atcaaaatca ggatgcccat acaggcttac agttcgaagc gcgaattgcc caacggcagt
    60601 ctctggtaaa acagctgcat gaacgccagc ttgatcttct tattaccact gaagcgccca
    60661 aaatggacga atttagtagt cagttgctgg gatatttcac tttagcgctt tataccagtg
    60721 ccccttcaaa actaaaggga gatcttaatt atctgcgact tgagtggggg ccagattttc
    60781 aacagcatga ggcaggtttg atcggtgctg acgaagtgcc cattctgaca accagttctg
    60841 ctgaactggc acagcaacag attgcgatgc ttaatggttg cacctggcta cccgtcagct
    60901 gggcgcgtaa aaaaggcggc ctgcataccg ttgtcgatag cacaacactt tcacggccgc
    60961 tttatgccat atggctgcaa aatagcgata aaaatgcgtt gattcgcgat attttgaaaa
    61021 ttaacgtgct ggatgaagtg tattaatatg aatggctggc aaggatgccg gtagaaggat
    61081 ttacttcgga gagggttatt tcagataaaa aaatccttag ctttcgctaa ggatgatttc
    61141 tggcaggggc ggagagactc gaactcccaa cacccggttt tggagaccgg tgctctacca
    61201 attgaactac gcccctaatt agggtggcgg aacggacggg actcgaaccc gcgaccccct
    61261 gcgtgacagg caggtattct aaccgactga actaccgctc caccgaattc ttttacaacc
    61321 accggtttta tgaccggctt
//
LOCUS       DAZKIV010000010        59930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZKIV010000010 DAZKIV010000000
VERSION     DAZKIV010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.010"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHOT6_000673"
     CDS             complement(<1..209)
                     /locus_tag="ACHOT6_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9945942.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOT6_000674"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHOT6_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFP9945943.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOT6_000675"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHOT6_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFP9945944.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOT6_000676"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHOT6_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFP9945945.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOT6_000677"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHOT6_000677"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFP9945946.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOT6_000678"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHOT6_000678"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFP9945947.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOT6_000679"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHOT6_000679"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFP9945948.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOT6_000680"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHOT6_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9945949.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOT6_000681"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHOT6_000681"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFP9945950.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOT6_000682"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHOT6_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFP9945951.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOT6_000683"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHOT6_000683"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFP9945952.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAIGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOT6_000684"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHOT6_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFP9945953.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHOT6_000685"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHOT6_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFP9945954.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPAKLWHLRRSIFGVGAAQVLLSAALL
                     AGLLMLTHFSWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     LPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOT6_000686"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHOT6_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFP9945955.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOT6_000687"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHOT6_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9945956.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDDAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOT6_000688"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHOT6_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9945957.1"
                     /translation="MAQITTTDANEFSSSAEFIPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSENPAQAQHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPIDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLTQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOT6_000689"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHOT6_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFP9945958.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOT6_000690"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHOT6_000690"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFP9945959.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOT6_000691"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHOT6_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFP9945960.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHOT6_000692"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHOT6_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFP9945961.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOT6_000693"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHOT6_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFP9945962.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILSKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOT6_000694"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHOT6_000694"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFP9945963.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOT6_000695"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHOT6_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFP9945964.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAEKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOT6_000696"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHOT6_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFP9945965.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOT6_000697"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHOT6_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFP9945966.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOT6_000698"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="ACHOT6_000698"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFP9945967.1"
                     /translation="MFKSTLAAMAAVFALSALSTAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOT6_000699"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="ACHOT6_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFP9945968.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOT6_000700"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="ACHOT6_000700"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFP9945969.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILQPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOT6_000701"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="ACHOT6_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFP9945970.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="ACHOT6_000702"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="ACHOT6_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFP9945971.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cysG"
                     /locus_tag="ACHOT6_000703"
     CDS             26709..28082
                     /gene="cysG"
                     /locus_tag="ACHOT6_000703"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFP9945972.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28329..28496
                     /gene="yhfL"
                     /locus_tag="ACHOT6_000704"
     CDS             28329..28496
                     /gene="yhfL"
                     /locus_tag="ACHOT6_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFP9945973.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28791..30128
                     /gene="frlA"
                     /locus_tag="ACHOT6_000705"
     CDS             28791..30128
                     /gene="frlA"
                     /locus_tag="ACHOT6_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFP9945974.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            30149..31171
                     /gene="frlB"
                     /locus_tag="ACHOT6_000706"
     CDS             30149..31171
                     /gene="frlB"
                     /locus_tag="ACHOT6_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFP9945975.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31221..32051
                     /gene="frlC"
                     /locus_tag="ACHOT6_000707"
     CDS             31221..32051
                     /gene="frlC"
                     /locus_tag="ACHOT6_000707"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFP9945976.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFASDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            32048..32833
                     /gene="frlD"
                     /locus_tag="ACHOT6_000708"
     CDS             32048..32833
                     /gene="frlD"
                     /locus_tag="ACHOT6_000708"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFP9945977.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGVVIVTLGENGSIAWDGAQFWRQTPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32933..33664
                     /gene="frlR"
                     /locus_tag="ACHOT6_000709"
                     /gene_synonym="yhfR"
     CDS             32933..33664
                     /gene="frlR"
                     /locus_tag="ACHOT6_000709"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFP9945978.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33816..34901)
                     /gene="yhfS"
                     /locus_tag="ACHOT6_000710"
     CDS             complement(33816..34901)
                     /gene="yhfS"
                     /locus_tag="ACHOT6_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9945979.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34913..36216)
                     /gene="yhfT"
                     /locus_tag="ACHOT6_000711"
                     /pseudo
     CDS             complement(34913..36216)
                     /gene="yhfT"
                     /locus_tag="ACHOT6_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
     gene            complement(36228..36581)
                     /gene="yhfU"
                     /locus_tag="ACHOT6_000712"
     CDS             complement(36228..36581)
                     /gene="yhfU"
                     /locus_tag="ACHOT6_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFP9945980.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36592..37470)
                     /gene="php"
                     /locus_tag="ACHOT6_000713"
     CDS             complement(36592..37470)
                     /gene="php"
                     /locus_tag="ACHOT6_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFP9945981.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37467..38693)
                     /gene="yhfW"
                     /locus_tag="ACHOT6_000714"
     CDS             complement(37467..38693)
                     /gene="yhfW"
                     /locus_tag="ACHOT6_000714"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9945982.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQSVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGCFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38694..39857)
                     /gene="yhfX"
                     /locus_tag="ACHOT6_000715"
     CDS             complement(38694..39857)
                     /gene="yhfX"
                     /locus_tag="ACHOT6_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFP9945983.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNPLEA"
     gene            complement(39941..40303)
                     /gene="yhfY"
                     /locus_tag="ACHOT6_000716"
     CDS             complement(39941..40303)
                     /gene="yhfY"
                     /locus_tag="ACHOT6_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFP9945984.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(40320..41225)
                     /gene="yhfZ"
                     /locus_tag="ACHOT6_000717"
     CDS             complement(40320..41225)
                     /gene="yhfZ"
                     /locus_tag="ACHOT6_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFP9945985.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGGSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41494..42498)
                     /gene="trpS"
                     /locus_tag="ACHOT6_000718"
     CDS             complement(41494..42498)
                     /gene="trpS"
                     /locus_tag="ACHOT6_000718"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFP9945986.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42491..43249)
                     /gene="gph"
                     /locus_tag="ACHOT6_000719"
     CDS             complement(42491..43249)
                     /gene="gph"
                     /locus_tag="ACHOT6_000719"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFP9945987.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43242..43919)
                     /gene="rpe"
                     /locus_tag="ACHOT6_000720"
     CDS             complement(43242..43919)
                     /gene="rpe"
                     /locus_tag="ACHOT6_000720"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9945988.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43937..44773)
                     /gene="dam"
                     /locus_tag="ACHOT6_000721"
     CDS             complement(43937..44773)
                     /gene="dam"
                     /locus_tag="ACHOT6_000721"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9945989.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44880..46166)
                     /gene="damX"
                     /locus_tag="ACHOT6_000722"
     CDS             complement(44880..46166)
                     /gene="damX"
                     /locus_tag="ACHOT6_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFP9945990.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46258..47346)
                     /gene="aroB"
                     /locus_tag="ACHOT6_000723"
     CDS             complement(46258..47346)
                     /gene="aroB"
                     /locus_tag="ACHOT6_000723"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFP9945991.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47403..47924)
                     /gene="aroK"
                     /locus_tag="ACHOT6_000724"
     CDS             complement(47403..47924)
                     /gene="aroK"
                     /locus_tag="ACHOT6_000724"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFP9945992.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48325..49563)
                     /gene="hofQ"
                     /locus_tag="ACHOT6_000725"
     CDS             complement(48325..49563)
                     /gene="hofQ"
                     /locus_tag="ACHOT6_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFP9945993.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSDQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49475..49879)
                     /gene="hofP"
                     /locus_tag="ACHOT6_000726"
     CDS             complement(49475..49879)
                     /gene="hofP"
                     /locus_tag="ACHOT6_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFP9945994.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(49869..50309)
                     /gene="hofO"
                     /locus_tag="ACHOT6_000727"
     CDS             complement(49869..50309)
                     /gene="hofO"
                     /locus_tag="ACHOT6_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFP9945995.1"
                     /translation="MNMFFDWWFAISPRLRQFCWAVWLLMLVTLIFLSSTHHEESDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(50293..50832)
                     /gene="hofN"
                     /locus_tag="ACHOT6_000728"
     CDS             complement(50293..50832)
                     /gene="hofN"
                     /locus_tag="ACHOT6_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFP9945996.1"
                     /translation="MNLPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50832..51611)
                     /gene="hofM"
                     /locus_tag="ACHOT6_000729"
     CDS             complement(50832..51611)
                     /gene="hofM"
                     /locus_tag="ACHOT6_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFP9945997.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQQFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51731..54283
                     /gene="mrcA"
                     /locus_tag="ACHOT6_000730"
     CDS             51731..54283
                     /gene="mrcA"
                     /locus_tag="ACHOT6_000730"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFP9945998.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54448..55008)
                     /gene="nudE"
                     /locus_tag="ACHOT6_000731"
     CDS             complement(54448..55008)
                     /gene="nudE"
                     /locus_tag="ACHOT6_000731"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFP9945999.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55328..57463
                     /gene="igaA"
                     /locus_tag="ACHOT6_000732"
     CDS             55328..57463
                     /gene="igaA"
                     /locus_tag="ACHOT6_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFP9946000.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            57528..58196
                     /gene="yrfG"
                     /locus_tag="ACHOT6_000733"
     CDS             57528..58196
                     /gene="yrfG"
                     /locus_tag="ACHOT6_000733"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFP9946001.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58207..58608
                     /gene="hslR"
                     /locus_tag="ACHOT6_000734"
     CDS             58207..58608
                     /gene="hslR"
                     /locus_tag="ACHOT6_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFP9946002.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58633..59511
                     /gene="hslO"
                     /locus_tag="ACHOT6_000735"
     CDS             58633..59511
                     /gene="hslO"
                     /locus_tag="ACHOT6_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFP9946003.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(59646..>59930)
                     /locus_tag="ACHOT6_000736"
     CDS             complement(59646..>59930)
                     /locus_tag="ACHOT6_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9946004.1"
                     /translation="TCIEKDACVLVEQDLNSDGQAERILFAFNDDRVIVYGFDSDRKE
                     WDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGETLEINLSK"
BASE COUNT        14339 a        16316 c        15108 g        14167 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcact
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 acacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaggaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcaa ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caatagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggagcac cgccaaatcc tgaaacagca acaccgagaa gcccaactgc ccggattcgc
     9061 tgcgattcat ccctttttcg cgcatcagtt gtagtgccat tgcggtcgaa gacatcgcaa
     9121 gaccgatgcc gcccaccacc gccgcctgcc atgagaaatg ggttagcatc aatagtcccg
     9181 ccagtaatgc cgcgcttaac agcacctggg ctgcgccaac gccaaaaata gagcgccgca
     9241 aatgccagag tttggcaggg tttaactcca gcccgatgat aaacatcagg aacaccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caatcgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaactg gaagcgcagc tgcacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtctcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgacctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 cgcgccaaaa gagaatgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcatccc catgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct tgcgtggagt gaaaaccctg cacaggcgca acataaacct
    12301 cgtctggtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgagtttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaagaag gcaatgatct cccgattgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcacgc
    12721 aagctggcag cctaccccgg aacactgccg attaatctca cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatca tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccctgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacgtc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cggcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctccctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggtaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gctggggcca ttgcacgcaa aattgcttcc
    16021 tgcatcgctg ctcgccgcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accattgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gcgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgattacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gagcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcaaatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacaggc cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaacgcag
    18241 aagcaatttc cgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgcact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg cagccgtcac gccgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcactgc tttcactgcg tggagatcgt
    18541 taaagggaac gtggatgatg tctgccggtt tcggcccaaa gccgtcggaa tattttggct
    18601 gcccacccac cgaaacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacgggcta tgacgcacac aggcgtaatg acgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gttgcctcaa
    18781 tcagtttacg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga ataaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcataatca ccctgttacg
    19081 cataaataaa tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ctatgtgtaa tcggcgaggt tttgccgtgc atgacctttt cggcgcgcac aactttgccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccgggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccaacgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatcaataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gtgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tatccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacatt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gttttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ctgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgttgttgc tggcggatgt tcagccggtt
    20281 acgcatgata tcaaggcctg gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct acaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgagaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggccacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggt
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcgacg gcgatgttca ttctcggggt ggtcagcggc
    21781 attactatgt cgattggtac attcctgata acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttactga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcact gggcaaacat
    22021 gcaccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc cattttttgc acaaacatga
    22741 aatgtcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg acgtaccgtt ttttatgaca agctgatcat ggcaaccggt
    23221 tcctacccgt ggattccacc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg ttcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gaactggaag tcgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtattg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctcggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gccgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggtcac tcaggtactg
    24301 aacgcggaac tggcgaaaca ggggatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccgac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgcagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggca cctactcggt gatcccgcgt
    24601 tctccgggcg gtgaaatcac cccggaaggg ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaagat cactggctcc cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggaaatctg gcgtcagctg attgaagccg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactgcgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggt
    24841 gttggcgata gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcagta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gcgacgagca ggtcgcgatt ttccgcccgt atcacagcga ccaggtgttt gcgatcagca
    25561 acatcgatcc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tcttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggta gcggtctcct ctggcggcac ctctccggtt ctggcacgcc
    27121 tgttgcgcga aaaacttgaa tcactgctgc cgttgcatct gggccaggta gcgaaatacg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgggtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgatcgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaaccacc gaacagttaa tcaacgaacc gcttgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacata gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28261 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28321 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28381 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28441 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28501 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28561 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28621 tttgataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28681 cacattacat aacatcatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28741 tgactgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28801 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28861 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28921 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28981 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    29041 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29101 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29161 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29221 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29281 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccccta
    29341 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29401 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29461 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29521 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29581 cgccgatttc cgacgccctg acctggatcc ccgcactcgg cagcaccgct gggatctttg
    29641 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29701 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29761 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29821 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29881 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29941 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    30001 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gtcatcgtta
    30061 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30121 tgtcgtaatg ttatctggag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30181 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30241 ggtgcacacc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30301 ctgcggatcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30361 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30421 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30481 gctggagctg ggccgggcct gcggcgcact cactgcggcg ttcaccaaac gcgcggacag
    30541 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30601 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30661 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30721 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30781 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30841 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30901 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30961 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    31021 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31081 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31141 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31201 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31261 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31321 gcggtcgccc gcacgcgttc gcgtcggact taaaagcggg cggcatcaaa caaatcaagg
    31381 cgctggcgca gacgtatcag atgccgatta tcggctatac gccagaaacc aacggctatc
    31441 cgtataacat gatgctgggc gatgaacata tgcgtcgcga aagcctcgac atgatcaagc
    31501 tggcgatgga tatggcaaaa gagatgaacg cgggttatac gctgatttcc gcggcccacg
    31561 cgggctatct cacgccacct aatgttatct ggggacggct ggcagagaac ctaagcgaac
    31621 tgtgtgagta cgcggaaaac atcggcatgg atctaatcct cgaaccgtta acgccgtatg
    31681 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31741 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31801 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31861 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgagggaa ctgatgcgcg
    31921 atattatcga ccggggctat gagggttact gtacggtgga gctggtgacg atgtatatga
    31981 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    32041 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32101 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32161 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32221 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32281 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32341 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32401 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32461 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32521 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32581 gaaagcgatt gttgcccgtg gcgcaggcgt ggtgattgtc acgctgggtg aaaacggcag
    32641 cattgcctgg gatggcgcgc agttctggcg tcagactcct gaaccggtga cggttatcga
    32701 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32761 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32821 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32881 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32941 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcgac tgctggatga
    33001 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33061 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33121 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33181 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagg cgacgaaaga
    33241 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33301 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33361 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33421 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33481 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33541 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33601 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta actattgata ataaaagaca
    33661 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcgttgaat
    33721 tttacatccc gtacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33781 cagtacggaa tttagcttga cggtgctatc cagcattaaa tactggcaat actctcacgc
    33841 aaaatccgca gcaccgtctc ttcaccgctg cgattcgggt taatgcgaat cgcacaatgt
    33901 tctgattgtg gattcgcctg gcgaaacgtt ccggaaaggc gataaaagag cggcgggatt
    33961 tcatatttcg actctgcacc caccgggtaa ggcaaggcac cgcgcttttg cgcctcttcc
    34021 agcactctgg cggcaatcgg ctgatgaaac tcgacaatca acaccttcga ctgcgcatta
    34081 gcaatcaccg cgcttttcac ttccggcacc gcaccaccgt taagcaaagc cagcaaccgt
    34141 tcagataccc ctgcctgcac cgcgtgcatc actggcgcaa acaccagacc acgcaatact
    34201 tccagcgcct gtgcgccctg gatctggcta ccgccggagt aaagcgtggc gcgaatacgg
    34261 ttgataacat cagcatcgcc gaccacagca ccaacaccct ctggcccaaa tagcttgaag
    34321 caggaaaatg tcgagacatt cgcgccgcat tcacagccga ttcgcgccac cttcatcacc
    34381 gcatagttgt catcggttaa cgctggaaca cctgccgcgc gcaacgttgc cagcacatct
    34441 gccagcacgt agctgtcctg cggctgctgg cgcgtatgct gcaccagcgc cgcatccggt
    34501 tgttgctcgt cgacgacctg cttcagtgcc gacaggtcat tgaaatcaac agtaataagc
    34561 gtcagcccca tctgctcaat aataacccgt gtcgtcgggt aaacaggcgc gtcatgcacc
    34621 agaagacgct gccccggttt gagcaaagcc gccagcccgg cgcgaatcgc gccagtcccc
    34681 gcgccctgca ccagcgccgc agcctgtgcg tgaaatgcat cagccagcac ctgttccaca
    34741 cgctgggtaa cgcgcggttg attcagtcct ggcgttaagc ctaaatcacc gccagtaaga
    34801 aactcgctgc cggggaaatg gcgacaaatg ctatccacca gcgcaaactg cttttgctgc
    34861 gcctcaataa tcgtcaggct ttgcagagga aacgtcttca taggtgcctt ccttaagccg
    34921 gaacgaacag gccaagccag taaagaacat tcagcagaat accggtgatc attactgcca
    34981 cgaccggtgc cgccattttc tgtaccggac ggcccaggga ttcgttgagg aagtaaatgg
    35041 caaccgcgat agagaatccg gtataacccg ccatcttaat tgctgcgaaa atcgaaccga
    35101 ccagcagcgc cacttccatc agcatattca tggcgttacg gatgttatcc gacgcattac
    35161 gcaccgacgg atagcgcccc agccatttgc cgatcgaacg aagcaataaa acttctgccg
    35221 aaataaccac tgcacctaat accgctgcaa ccatcggatt cggcgagaga tagcccaccg
    35281 cataaacaaa ggtaaagccc gcaactgcat acacacccgt tgctaacgcg gtggtggcaa
    35341 tcaacggcac aaaccccagt ccgcgcataa attctgccag agccgcctga ttaatcagcg
    35401 tttgcgattg ttccggcgtt acgcctgcgg aatatgcttt ctccagtgtg aagatcgaca
    35461 cttcactgcc agcaaaaatc ttcatgctgg caacggcggc aatcaatgct cccacgatgg
    35521 cgatataggg taagtttttg ataatccgtg acgtgcgttc ttcaaacacc gaaagcccgc
    35581 tggcatcaat gtcattttca tcacgatgac gcaggtcgtg agttatcgcg atccccagca
    35641 gcatcaccat gccaataaag atttcgatgg attcagggtt aagatgtggg aaatagcgca
    35701 cgactaccac acgggtcatc agtaccacca cggcggcgat cagactttgc ttccagccaa
    35761 actggtagaa aatcgccacc agcgggaaca gtgcaaaagc tgaaaccacc ggcgagctta
    35821 attcccccag gctacctaat acatccaccg gcagcgcggt cagcagctgg tttactggca
    35881 acaggcaagt aaggatcaac acgccccaga tagcgccaag accaacgcca tcaggctatt
    35941 tatcgccagg acgccgagga tatcggtagg aagaaagagt aaccatgcgt tgagcagccc
    36001 ggttttcagg gtaaaagaga tccccaccga ggccacaaaa ccaatgctca gaccaaaagc
    36061 gatactcccc gcctcgcgac ggttcatata gccttcaatc agttgcggca ggatcgggcg
    36121 gatgccgtca tgaaaaacag ccgccgagcg atgcgccaga agcgatgtca tacccgtcag
    36181 gcacgccacc acgataatct gaatatacag atccatagtc gtgcctttta tttcagatgg
    36241 ttaatcagca tcggaatcgc gtgttcgacg tgctcaacgg aaaggccaaa cgccactttc
    36301 ccttcggcga tcattttggc gatatgctcg tctttcgctt taatgcccgg cttggcaata
    36361 gtgcaacttt tgttatagcc aatcaccgcg atggcaatcg acaatgccgc gcccgcaccg
    36421 gtattacacg cgccgatgta ataatccagt tgcccggatt tcaccttcat tgccgcttcc
    36481 atgtcgttgt gaatgaaaac ttcaaaacag ccaggagccg tcgcttcaat agttttttta
    36541 atctgctcac gctgtaagcc tgcaacgcca atctttttca tgagtctgtc cttattggaa
    36601 aaattgagag ggattttcac gtaacatcac atccacatcg gcctgactga atcctgactg
    36661 gcgcaattgc ggaataaagg tggtcaataa atagtcatag ccataaccac cgttggcttt
    36721 taaatgggag cggcgcgtaa tatccatcga cagcatgacg cggttcagca acccacggtc
    36781 gcgtagcgcg tggagcatcg caatgcgctt ttcgtccggg tagtaactgt tcttgccgat
    36841 ggtgtcgaac tgcacatacg cgccgagatc gatcatcttc aaaatgttgt cgaggttgtc
    36901 tttcagatcg cagtgaccaa cggtgacgcg cgaaagatca actccgtggg cttgcaacag
    36961 agccagttgt tccagcccca tggtgctgaa cgacgtatgc gtggagatcg ggcgtccggt
    37021 ctggttatgc gccagcgcag cggcaataaa taccttctct tccagcggcg taatctttcc
    37081 ttcgctggag ccgatctccg cgatgatccc ggctttcagc tccgtgccat cgataccctg
    37141 ttcaatttca tcgaccatct cctgcgccag ttcctgcacg ctgcgggtca ccacatgttc
    37201 cgggaaaaac gcatcctggt aataaccagt acaggccacc acgttgatcc cggtttcgcg
    37261 cattacatcg agcataaatt gcgcattgcg ccccatgtaa cggttggtca tctcaatcac
    37321 attacgtaca ccccgggtca tcaggtcgtt catctcctgg caaatgaacg catactgatc
    37381 aaggcggcag tccacgttgt ttttaaagcc ggagagatca atatgcagat gctcatgggc
    37441 gagggtgtaa cccgtcggat caaaactcat agggtgtctc ctgcaaaccg gagggaggaa
    37501 agaaaagagc gaccattttg tggcggtggc gcgcggaaaa attcacacac ggtagccccc
    37561 acatcagaaa gcgtggtgcg cacaccgagc tgcgtagcga tcatcccttg ctgataaacc
    37621 agcactggca ccacttcgcg ggtatggtgg ctgtgaccaa tggtcggatc gttgccgtga
    37681 tccgccatca cgaccaggca atcatctggc tgcatcgcct caacaagccg ggcaaggtta
    37741 cggtcaacga cctgcaaacg ttcggcataa cgtgcgacgt cttctgcatg accagcgagg
    37801 tcggtttcct gaatgttggt gcaaataaac gccgtcggat gggtgttaaa ttcgttgagg
    37861 gtgatatcca taatccgctg gctatccacc agattttgcc agctcacgcc ataaggattg
    37921 ttgacgatat ctgccacctt acccaccagc acggttggca cgcctgcttc atacagtttt
    37981 tgtggcacct gcactttttc atcgacgcca tagcccatat gcacgacctg gaaaccgttg
    38041 tcataagcgc cagaacgcgg cgcattaatc ccaataaagc acccttcttt gctttctgcg
    38101 gcatcgagaa tgcgttgact gtcggttaac aggccgccaa atgtaatgac ccgaccgacc
    38161 tgtacctgct cacgcacaat acgaccaatt ttgattgcgt cgtcaaaaga gatcacagag
    38221 agattggcgg taatgttata gacctggcct aaatccgcct cgagattatc gccaatcgca
    38281 accgactgat tgacccacag aaattgcaga tcatcgccac ggcgctccac ctgccaaccg
    38341 gcggatacta atgcctgctc aacacgatca atcacatcgc gaaagggcat ccgcagcggc
    38401 ggtaacgggc gcgtgcctaa aatttcctga tgccccataa aggtatcgcc accttcatgt
    38461 tgcagctctg ccacgcccca ggttgcggag tctgacggct gcatatcgcc tggcgcataa
    38521 cccaatgcgt tgattagccc cagcttctcc agcgttggta gctgcaaatg cggcaactgg
    38581 ctcaggatgt gaccacaggt attcgccccg gcatcctgcg gacgcaccag cgtgacatct
    38641 ttcattgcgc ctacgccaaa gctatcaatc actaacacca caaatcgcgc catttacgcc
    38701 tccagagggt tgccgagact gtcataacga ccaacgattt tcggttcgcc acggtgaata
    38761 ccggacacca gcaccacatc gctacgggtg acaaaaatct gcgtgcggaa acagagcacc
    38821 actgcactgc tcaccggata ctcgcctgcc agcggcaggg tgtagtcgat actgctgtca
    38881 tccacagttt tgagattggt ttcagtaatc ttttgatttt ctggcgtaaa caccagcgca
    38941 tgttgcgcat gaccacgacg gtagtaaccg ccgccgtagc agtagctgtc gccacggaaa
    39001 tgatgggaga tttcacttaa ccagagcatc gcgatacgtt caggctgatc gccctgctgg
    39061 tttgccggaa tagtgcccgt cagcgcatga ccgggttcgg catgagtcac accgtattcc
    39121 gccagcaatg gcagcgaagt gcagctggtc gctgaaggcg cgttcagttg ctcaagtgca
    39181 ataccagatt tcgccagttg atcccgtgcc tgtatcagcg tgtgaagatt cggtgtcggc
    39241 aaaacttttc cggcagcctc atcccaaagc aggcaaggga aatgggtaag tccggctaaa
    39301 tgcagccccg gcagtttctt aatttcagca accacctcgt gcagcgaatg ctgtacaaaa
    39361 ccgctctcct gccccggata aagaaaatca tcgtcgctat aaactttgag cagcacaaac
    39421 tgcacacgtc cggccttcac cgctgccgca gaaacttccc gcgctttgtc gagagtaaac
    39481 acggtgatga cgtcggtgcc ctgttcaacg gcgtcagcaa cctgatgaca agggatttgt
    39541 accagatgcc cctgatgcgc caccgacaaa ccagcgcggc gcatgactcg cgcctctttg
    39601 taatccaccg ccacaatgcc gctgtaacct aatgccagca atttttccgc cagccatggg
    39661 ttacggccaa actgcttggt catcagatac agttcaatgc cgtaaagccg cgccgtctca
    39721 atcagtcgct taccgttttc cagtacctga tccacgtcga tcacccagct gtccggggcg
    39781 atcttgccct gctgccacaa acttagtgcg gcggaaatca gcgccgggtt ctggcgtttc
    39841 aatgcttcta caaacatctt gttgcctcgt ttctgttcct tgaattaaat attcattttt
    39901 ttgaatattt aggttgtgcg acgtggattt gtcgggagct tcaaacctct tcgttagcag
    39961 ccatccataa tccataaagg ttggccagca aatagccttc ttcacacggg ttaacttcca
    40021 gtgcgaattc cttcaacaac acctgatgca attgcacaac ggcttgccag tggctggatt
    40081 gcgccagctc tgccagcaat tcgttatcca gcggctctat ttcttcacca cggcgactgc
    40141 gcatcagtgc actcgccata tgtgtcatcg ccatcgttcc ttgctcactg cgcaccggca
    40201 gatggcactc tgtttccagt acgttgacga cctgcatcat gcctttgcag atgtccttat
    40261 caataacgcc tgcctcgcaa agcaggttga gtctggtttc catatcagat gcctttcgtt
    40321 taataactcg gttcctgttc cccactcact acccgttgtt gatgggcgag cagggcgtgt
    40381 ttatctacaa cggcacgcag cagttgttgc attgggtaat catcgactcg cgtcaggacc
    40441 acggcttcgg tggcctgtaa aaatcgcgga tcgtctgtga gcggcgtcgc ctctaatccc
    40501 agcatggtca gttcgttttc cgccaccacg ttccagatca ccgcatcgac atcgccttta
    40561 acaatgcgtt gtaaactctc gtgataagag agatcgactc gttccacatc actaccgcca
    40621 aaaaaaacat cggtcatgat tttctgatcc gccgaacggt tatccagccc cacgcgcttc
    40681 acgtttgcgg actcgccttt acggcaaatc aactggtgct cgccaacgta ggtgtgcggc
    40741 cccaactcca gcgcgaggca taaccctttt tgcgtgagat aactttccgc cgccagtcgg
    40801 gaaaccaccg ccatgtcata cacgccgtta agtaaacact ccacgcgaat atccgcgccg
    40861 cgcatatgcg catagtaaaa aggaatgcca tcaaactgag ctttcagccc gctcgctaaa
    40921 ccttcgtaca agcgggtata gggcaaaggc atcgcacata ccacgttgtt gatatccaca
    40981 tgactcagca atgctttgtt atccatctcg accagataac tgccattgcg cccacggcgt
    41041 tcaatccgta tcgctccgct tgattccagc gttttcaacg cggcctgcgt caggccaacc
    41101 gatgaacggc attcagttgc cagctcatct atggttttca acctattacc gcatttttca
    41161 cccaacaaat aacgggccag cgtcgtgata acgacgcctt cttttttgat aaacgttcga
    41221 cgcataatat attttcaata aaatgaatgt ttatatcttc attaaaatga agataagtgg
    41281 caactgtgag atgtggaaac ggcgaggcgc ttcacatttt ttcggattat tgcaaatgag
    41341 gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta ttcgacaatg
    41401 cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac cctctcccca
    41461 aaggggcgag gggaccgtcc gagcgttttg atattaaggc ttcgccacaa aaccaatcgc
    41521 ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg cgccgtcttt
    41581 catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt cctgcaattc
    41641 agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat acatcttgcc
    41701 ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa ggatatccag
    41761 caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg gctcgtcgga
    41821 gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca gcaggccgat
    41881 aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca gcagcgacat
    41941 tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct cgccatacag
    42001 cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct ggtcttcacc
    42061 caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca ccggatagtc
    42121 aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa actgggtcat
    42181 gcggctcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact gcgcatgttc
    42241 tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac aagccagata
    42301 caaggccagc gtatccagcg tcgctttacg cagcttctgt gcatcctggc gcacggtgat
    42361 cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt ttacccactg
    42421 acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg cgccactaaa
    42481 aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc cctaatgcgg
    42541 gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg atagcctcgc
    42601 cgtagttata tccgtaggtt aagccaactg atgggcaacc tgccgctttt gccgcctgaa
    42661 tatcattgcg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc atccgctcag
    42721 ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg ccgccgataa
    42781 ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg aacggcgtcg
    42841 gtttgttggt gaccaggcct agcggcaggc ctttagcctg caacgcgccc aacgtatcgg
    42901 caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gctatagtag cgatcgaaca
    42961 gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg ggcggtttac
    43021 ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg cgctccatca
    43081 gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg atgggcaact
    43141 ccagcgcata cagcgccata tctaccgcag cagcaagacc gggagcactg tcgaccagcg
    43201 taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg acttaccttt
    43261 gccagttcac tgcgcatttc atcaatgact tttttgtagt ctggctggtc gaagattgcc
    43321 gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt gttcaccttc
    43381 acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg gcgacgtact
    43441 tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc aggattgacg
    43501 gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct cagaggtgtc
    43561 gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg cagcgtgcgg
    43621 tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc ggcagcgaaa
    43681 tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg ggcggtaatg
    43741 ccatagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg aacatagtgg
    43801 ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt tgcggtatct
    43861 tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata ctgtttcatc
    43921 cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg tacaaagcca
    43981 gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt cgaactttga
    44041 cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg tgattggaga
    44101 tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga tgcgcttgtt
    44161 gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca gacagcggtg
    44221 cataaggcgg atcgcaatag acgacggatg catcatctgc gcgcgccatg ctatcggcgt
    44281 aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac aactctgctt
    44341 ccgggaaata gggttttttg tagcggccaa acggcacatt aaactcaccg cgcagattgt
    44401 aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc gcccgacgga
    44461 acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg gcgcaatttg
    44521 tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc atcttcacaa
    44581 tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga gaaaagtcgg
    44641 tgttgagaaa caccgacccg gcacctacaa aaggctcaac caaacattcg cccttgggca
    44701 aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc aaaaaagcgc
    44761 gatttttctt catgctgact aactaattac accttctccg gctgtggaga aagctccgac
    44821 agcatccagc gcttcaggca actcccgcaa cgataaacca ttgcgggaga taaccttgat
    44881 tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt ggcctggaca
    44941 tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta cacgccagaa
    45001 accaggacat accacggctg accattacgc gtcgtttcat agacaacgta gtttttcaga
    45061 ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct cagctgcaga
    45121 gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt cttccctcca
    45181 gcggctggtg ttgccgtggt ttgcgccggg gatgccgttt gtactggagc cgtagtcgcc
    45241 gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc cacaggagca
    45301 gctggttcag tacgcttcgg cgtctgtgct accggcttcg gttccgtttt cacggttgct
    45361 tgcggttttt tcggttcaat caccgcctgc tgacgagctg gacgcgtagt ggccggacgt
    45421 tcagcagttt gcgttttcgc cgtgtcacgc gatgcattgc cattgcgtac tggcgcgaca
    45481 gttgctggtt cagtcggcaa tgtagaattg accgccacat tgttcaactg ttgctgattt
    45541 tgtggttggg tcagcgcatt gttcaggtca ccctgcactt caacacgttg ttgaccatcc
    45601 gttgccgccg gggtttgccc ttgagtcggc gtagaagaga tcggcggcag agaaacatcc
    45661 tgctgagtat tttccgcaga cgtggttccc ggcgctggct gcacgccatt ggcctgatcg
    45721 gtcgcattgc cagcaagatc aatactcttc tcgccagacg cggtttgatc gctggaagag
    45781 gtcgaggggg cttttagcgc agaaccgata ccgatgatca acagcagtag aaccagaatg
    45841 ccgacgccca tcatcatata ctgacgagaa gcgggtttac tggctgcttt tttgcgctta
    45901 cgcggacgac gctctacgcg ctcttcatcc acggtttcat cttcggattc gtcaatttct
    45961 tcttcgattt ccggttcctc attgcgctct ttttgcgcac gagtcggacg gcgatcgtca
    46021 gtgtcatcaa gttcaatatc atcaaaattg atctgcggtt cgccacgttc agtacgctca
    46081 gaacgttcag aagattgacg agaacgacca gtacgacgat cgctgggatc gggtttcagc
    46141 tcgtcttctg gtttgaattc atccatttaa caccccacta aaaggttaat gcttaccacg
    46201 ttgcaattaa cctgaagcta atagaccgct tgataagcgg cctgaccttt cttgttgtta
    46261 cgctgattga caatcggcaa tggcgttaag aacaagctcg tgcgaaacgc cgctgcgaac
    46321 ttcactctta ccaattgcca acggaagaat taagcgcatc tctcccgcaa ggactttctt
    46381 gtcacgcagc atatgcggta aatacgcctg cgcggacatt tcgcgcggcc cattgaccgg
    46441 taacccagcc cgcgtgagca gggttataat acgctgcgtt tcggcagaac taaactgccc
    46501 gagacgttcc gacgtccgcg ccgccatcac catacccgca gcgaccgctt caccatgtaa
    46561 ccaattgcca taccccattt cagcttcaat ggcatgacca aaggtgtgtc ccagattcag
    46621 taaagcacgt aacccggttt cgcgctcgtc ggcggcgaca acttctgcct tcagttcaca
    46681 acaacggcga atacagtacg ccattgccgg accgtccaga cgcaacaacg catccagatt
    46741 ctcttccagc cagttaaaaa acgcaccgtc aagaataatg ccgtatttga tgacttctgc
    46801 cagccccgac gctaactcac gcgggggaag cgttttcaga cagtcgagat ccaccaccac
    46861 tgaagcaggc tggtagaacg cgccaatcat gtttttaccg aggggatggt tgaccgcagt
    46921 tttgccgcca acggaggaat cgacctgcga cagtaacgtc gtcgggactt gaatgaaacg
    46981 aacaccgcgc tgataactcg ccgccgcgaa gccggtcaga tcgcccacta cgccgccgcc
    47041 aagcgccacc agcgtagtat cgcgaccatg cggcttttgt aacaacgccg taaagacggt
    47101 atcgagtaca gccaggcttt tatactgctc gccgtcaggg aggataacgc tatcgacgtt
    47161 aacacccgcc tgttcaagta cgccgcggac cttatcgaga tacagaggag ccagggtttc
    47221 gttggtgacc aacatgacct gctcgcccga tttcagcggt aagaatgaag ctggttcatt
    47281 aaacaaacca gatgcgatgg taattgggta actacgttcc ccgagagtaa cgacaatcct
    47341 ctccataacg cgacatccac cttaattact gtacccgcag acgagtgtat ataaagccag
    47401 aattagttgc tttccagcat gtgaataatc tggtttgcaa ccactttagc gctttgatca
    47461 tcagtacgaa tggtcacgtc ggcaatctct tcatacagcg gattgcgttc attggccaac
    47521 gcttccagaa cttcacgcgg cggtgtttca acgtgcagca acgggcgttt tttatcacgc
    47581 tgcgtgcgtg caagttgctt ttcgatggtc gtttcaagat aaacgacaac gccacgagcg
    47641 gaaagacggt tacgcgtttc acgggatttc acagagccgc cgccagtagc cagcacaata
    47701 ccctgtttct cggtcaactc attgatgacc ttttcttcgc gatcgcggaa gccttcttcg
    47761 ccttctaaat cgaaaaccca gcccacatca gctccggttc gtttctcaat ctcttgatcg
    47821 gaatcgtaaa attccatatt gagttgttga gctaactggc gcccaatagt gctttttccg
    47881 gcacccatag gcccaaccag aaagatattg cgtttctctg ccattttttc ggtactacta
    47941 agactattcg ttaatgataa acccgcttcg ctcagagagc gccgcaggac atgaactgaa
    48001 acctcataag atattgcgag agtcagactg aaaattatct caatactcca gcgggtttgg
    48061 caactgaata aatcacccat ccccctgcat atctggtcgc tgcaagcgcg ctgccttgct
    48121 accaccgctc tggcgataaa tcaccgggta agattagcgt aaaaaagaca gcaaaatgcc
    48181 gcctgaatga taaatcatca tcatggaacg caggcagcga agagcaccaa ctctcaaatc
    48241 ggtactcctt gtatgctaaa tacctgcgcg cgtcaaatag atgaaacacg ttcagcgtaa
    48301 aaacattacc gcttttacgg ctgtttactc actggaaacc agtcgtggcg tgataaacac
    48361 cactaactcg cgtcgttcat cttcttttcc gtcatgacga aataattgcc cgaaccaggg
    48421 aatgtcgcca agcaacggca cgctatcctg atccgattta tttttacggg taaaaatgcc
    48481 gcccagcgcc aacgtttctc cgcttttgac ctcgacctgc gtttcaatct cctgcttatc
    48541 aatcgccagc acttcgccat cggcctgctg tagcacctgc cccggaacgt tctggctgat
    48601 gtgtaatttc agccgaatgc gacctttttg taacaccgtg ggcgtgacct ccatccccag
    48661 gacggcctct ttaaattcca ccgacgtcgc gccactttcc ccgctggaaa cctgatatgg
    48721 aatttcgctc ccctgtttaa tgctggcagg ctgaagatgt gaggccagca gacgcggact
    48781 ggcgataata tccagctgct gtttttgttc gagcgcggaa agctcaagat ccagcaaacg
    48841 tccgttgatg cgcccaatgt taaaaccgac atgcgttgtc gccgtcgcta cggagaggtc
    48901 gctgccaagc gtggtgactt gcccaacgcc accagcttgt tgcgcatcgg ccagcgtcca
    48961 tttcacgcct aactcacgca aacttttttc attaatggtg acaatatgcg ccgacagctc
    49021 aacctgcccg accggaagat ccatttgcgc tacccactgt tcaagcgcgc ttaacgccgt
    49081 tttgttatct cgtagcaaaa ggcgattggt gcgtttatcg acggtcatac tccctttggc
    49141 actcagtagc ttctcccccg ctttcgccag ttctcccgcg tcggcgtatt gcagggttat
    49201 actgcgattt tccagcggca gatttgcctg cgcccgcgcc tgctccgcct cctggcgggc
    49261 gatattgtta ttctgccagg caatggaatg cactgagaga atgttgtctt cctgccgcgt
    49321 tatcagtccg gcgcttttca ctacagtttg tagtgcctgc ttccagggaa catctgttag
    49381 atgtaacgac accgtaccgc tgacgtctgg cgacacgacc aggttcaact tctcctgttc
    49441 agccagcgcc tgcaacacct gagctaccgg aacgtcatcc accatcagcg tcactttttg
    49501 cggctttgcc gcctgtacgc cgggtatcag catcaacagt agtgcggcta tccattgctt
    49561 catttgtatc tccttgccgt tgccacaacc attgtggcgg ttcgcagttt gtcccggttc
    49621 ccagcgttag tgcgtctggc gtcagctgta aaattgtcca accgttttcc agcacatcgt
    49681 tttgctgcac ccgtcgccat ttcttttgcc cgtcttttat tacaccgatg atgtgctcgc
    49741 ctcgccctac catcccctga tagcgccact ggctaagttc gctaatccgg catagatctt
    49801 ccggcggttt aaaagggtca cgcataccgg ttaaaaggca caatgcaata cctgccaaca
    49861 accagcgttt aaccctcatg cggtgtctcc agttgtagcg tgaacaaaag atcatcacct
    49921 tccacgctta acgaaaaacg gctcacgctg acgttgcgct ctgccagccg tgtaaatgcc
    49981 gacggcactg cttcccacag cgttttcaac gccaactcac ctccctgcgc ggatggatgc
    50041 caggaaacca gttgcgcgcc ggataactga aaatccagag gcgaaaaggg cagcgttttt
    50101 tcctcgctga agggagtggt gtctaccagg cgatacagtg tggcccactg ctggtgatga
    50161 cttgcccgta gtcgaattaa tgcgtcgctc tcttcatggt gtgtcgatga cagaaaaatg
    50221 agcgtaacta acatcagcaa ccagactgcc cagcaaaact ggcggaggcg gggtgatatg
    50281 gcgaaccacc agtcaaagaa catgttcatc gctaaccttc cttgttaact gatactcaaa
    50341 ttgccagcgt ccctgcgcat cctgctgcgt ggccccccgc tgattgagat gaaaagaagc
    50401 atcctggcga agtgacgttt ctagtgcgtt taacgcggta atgcttgttg taagcccctt
    50461 gatctccagc gttccctgct gccagcttat cgttgtcagc caggcgtgtt caggtaaaag
    50521 agccgccagt gcttccagcg cagattgcca gtcgcgggta aattgtcgct ggcgctgcct
    50581 ttgcaaacgc tgctcacgaa tttgttggcg ctccagcaaa cgtggtttcg ttatctgtaa
    50641 gctgtgggcg agttgttgtt ccccctgaag caaaacggcg tctacgcgag cttcggcgct
    50701 gcttgtcaga cgcagtatta gcgttatccc gacggccagc agcagaggcg caacgaacat
    50761 cagcaaccag aaacgcagaa aagcggtccg gcgttgctgt cgccagggca aaaaattaat
    50821 tggcaggttc atcagtacgc cttcccaagc gccagcccca gcgcgatggc aaagtctcca
    50881 cctggcggcg gtagcggcgg ctgacgaaca gaaacggcct cccagggatc aaatccgcct
    50941 tcgccacata tcgcgacgct gtcgggatca acggataacg ctgccgccag ctccttcgcg
    51001 ctagtcatcc ctaccgccag tttgcgcccc cagcgatagc gtgtcgccca cagccactgt
    51061 tcgttatcac gccaggccag acattgctga tgagaaggta aaaaaggcag gaattgctgt
    51121 aatgcactgg catccggggt gatcgcgctc acatgaacac gcaacctttc tgccagagta
    51181 agtagcgttg ccagctcttt gctttgcgcg gcagtcacgt tataggcggg gctgagtgag
    51241 tcttcgctgt aatcgaagcg cagggagtcc ggatccatat ccagctcgcg ggccatcgtc
    51301 cctgacagcc aggccgtttg ctcccgctca ccaagggaca tcgacgggcg cggaaatgac
    51361 cgctgtaatg tgcgactggc gggaaacgcc aacataatgt gatgacgctg cggcagttcg
    51421 cgactccacg gtaacaacgt tttagccagc tgctgcgcat caacaatccg cccatctttg
    51481 ataatgtcgt tctccagcgg caaccgccac cagcgttgca aaaagcattc ttttgctccc
    51541 cgcacgatcg caaccgctac cgcttcttgc tgttgtaaat gcaaaccaat ttgccagatc
    51601 ttaaatgcca ttgtgatgat ctccttatca cccgtcactc tgacgggtat atcaatgcgt
    51661 ctggcttgcc tttatactac cgcgcgtttg tttataaact gcccaaatga aactaaatgg
    51721 gaaatttcca gtgaagttcg taaagtattt tttgatcctt gcagtctgtt gcattctgct
    51781 gggagcaggc tcgatttatg gcctataccg ctacatcgag ccacaactgc cggatgtggc
    51841 gacattaaaa gatgttcgcc tgcaaattcc gatgcagatt tacagcgccg atggcgagct
    51901 gattgctcaa tacggtgaga aacgtcgtat tccggttacg ttggatcaaa tcccgccgga
    51961 gatggtgaaa gcctttatcg cgacggaaga cagccgcttc tacgagcatc acggcgttga
    52021 cccggtgggg atcttccgtg cggcaagtgt ggcgctgttc tccggccacg cgtcacaagg
    52081 ggcgagtacc attacccagc agctggcgag aaacttcttc ctcagtccag aacgcacgct
    52141 gatgcgtaag attaaggaag tcttcctcgc gattcgcatt gaacagttgc tgacgaaaga
    52201 cgagatcctc gagctttatc tgaacaagat ttacctcggt taccgcgcct atggtgtcgg
    52261 tgctgcggca caagtctatt tcggtaaaac ggtcgaccaa ctgacgctga acgaaatggc
    52321 tgtgatagcc gggctgccga aagcgccttc cacctttaat ccgctctact caatggatcg
    52381 tgccgtcgcg cggcgtaacg tcgtactgtc gcggatgctg gatgaagggt atatcaccca
    52441 acaacagttc gatcagacac gcactgaggc gattaacgct aactatcacg cgccggagat
    52501 tgctttctct gcgccgtacc tgagcgaaat ggtgcgccag gagatgtata accgttatgg
    52561 cgaaagtgcc tatgaagacg gttatcgcat ttacaccacc atcacccgca aagtgcagca
    52621 ggccgcacag caggcggtac gtaataacgt gctggactac gacatgcgcc acggctatcg
    52681 cggcccggca aatgtgctgt ggaaagtggg cgagtcggcg tgggataaca acaagattac
    52741 cgatacgctg aaggcgctgc caacctatgg tccgctgctg cctgccgcag tcaccagcgc
    52801 caatcctcag caagcgacgg cgatgctggc ggacgggtcg accgtcgcat tgagtatgga
    52861 aggcgttcgc tgggcgcgtc cttaccgttc ggatactcag caaggaccga cgccgcgtaa
    52921 agtgaccgat gttctgcaaa cgggtcagca aatctgggtt cgtcaggttg gcgatgcatg
    52981 gtggctggca caagtgccgg aagtgaactc ggcgctggtg tcgatcaatc cgcaaaacgg
    53041 tgccgttatg gcgctggtcg gtggctttga tttcaatcag agcaagttta accgcgccac
    53101 ccaggcactg cgtcaggtgg gttccaacat caaaccgttc ctctacaccg cggcgatgga
    53161 taaaggtctg acgctggcaa gtatgttgaa cgatgtgcca atttcccgct gggatgcagg
    53221 tgccggttct gactggcagc cgaaaaactc gccgccacaa tatgccggtc caattcgctt
    53281 acgtcagggg ctgggtcagt cgaaaaacgt ggtgatggta cgcgcaatgc gggcgatggg
    53341 cgtcgactac gctgcagaat atctgcaacg cttcggcttc ccggcgcaaa acattgtcca
    53401 caccgaatcg ctggcgctgg gttcagcgtc cttcacccca atgcaggtgg cgcgcggcta
    53461 cgcggtcatg gcgaacggcg gcttcctggt ggacccgtgg tttatcagca aaattgaaaa
    53521 cgatcagggt ggcgtgattt tcgaagcgaa accgaaagta gcctgcccgg aatgcgatat
    53581 tccagtgatt tacggtgata cgcagaaatc gaacgtgctg gaaaataacg atgttgaaga
    53641 tgtcgctatc tcccgcgagc agcagaatgt ttctgtacca atgccgcagc tggagcaggc
    53701 aaatcaggcg ttagtggcga agactggcgc gcaggagtac gcaccgcacg tcatcaacac
    53761 tccgctggca ttcctgatta agagtgcttt gaacaccaat atctttggtg agccaggctg
    53821 gcagggtact ggctggcgtg caggtcgtga tttacagcgt cgcgatatcg gcgggaaaac
    53881 cggtaccact aacagttcga aagatgcgtg gttctcgggt tacggtccgg gcgttgtgac
    53941 ctcggtctgg attggctttg atgatcaccg tcgtaatctc ggtcatacaa cggcttccgg
    54001 agcgattaaa gatcagatct caggttacga aggcggtgcc aagagtgccc agcctgcatg
    54061 ggacgcttat atgaaagccg ttcttgaagg tgtgccggag cagccgctga cgccgcctcc
    54121 gggtattgtg acggtgaata tcgatcgcag caccgggcag ttagctaatg gtggcaacag
    54181 ccgcgaagag tatttcatcg aaggtacgca gccgacacaa caggcggtgc acgaggtggg
    54241 aacgaccatt atcgataatg gtgaggcaca ggaattgttc tgattaaaaa ggcgcttcgg
    54301 cgccttttca gttggctgac aaaatgcatt tgtttatgcc ggatgcgggt gaacgcgtta
    54361 tccggctaac aaaatcgtga aaaatcaata tattgcagga accccataag cctgataagc
    54421 gttgtgcatc aggcaaactt cacgcattta cactcgcccc tgccctttca accattcgcg
    54481 cacgaggaac agcgcgctga cattacgcgc ttcgttgaag tcagggtctt ccagcaaatc
    54541 catcatatgc gccagcggcc agcgcacctg tggtagcggc tctggctcat cgccttccag
    54601 cgattccggg tagagatctt gcgcgaccac gatattcatt ttgctggaaa agtaagacgg
    54661 tgccatgctg agcttcttca aaaaagtcag atcgttcgca ccaaatccaa cctcttcttt
    54721 tagctcgcgg ttagcggctt cgtagacgct ttcacccgga tcaattaatc ctttcgaaaa
    54781 acctaattcg taggattcag ttcccactgc gtattcgcgg atcaggatca ggtgatcgtc
    54841 cacaatcggc acaatcatca ctgcttcccg gttggttgga cgcattcgtt cataaacacg
    54901 ccgcacgcca ttgctgaact ccagatccac gctctcgacg gtaaacagtc gggaacgggc
    54961 tacagtttca acattcagaa tggtgggttt ttgtaatgat ttgctcatcg tgggatctat
    55021 gctgtgaaat cagcggttat tgtgcgatat cggacacgct ttcggcaatg tgaattgcat
    55081 gttatttaca tttatgtaac ttaataaata atcgtcctca aatcaaatta aaagtcaata
    55141 ggttgaaata actccaggaa tttgctgata ttccgccttc agagggtttg ctatgatcag
    55201 cggttactgt gatgtgctta atgatgctca agttaaactc cacgcttgcc gatagccaac
    55261 cgcagaatca tgtattgtgt ctggtgcgac tgaccacgcc tgacagacta agtaagatgg
    55321 ggaaagcatg agcaccattg tgattttttt agctgctttg ctggcctgct cactacttgc
    55381 gggatggctg ataaaagtgc gatccagacg gcgtcagctg ccctggacca acgccttcgc
    55441 ggatgcgcaa acgcgtaaac tcacacctga agaacgtagc gccgttgaaa attatcttga
    55501 gagcctgacg caggtattac aggtgcctgg cccaacggga gctagcgcgg caccgatctc
    55561 tctggcgctg aatgccgaaa gcaacaacgt catgatgctg acacacgcta tcacacgtta
    55621 cggcatctct accgacgatc cgaataaatg gcgttactac ctcgattcgg tagaagtcca
    55681 cctgcccccc ttctgggaac agtacatcaa cgatgagaat accgttgaac tgattcatac
    55741 cgattcgctg ccgttggtta tttcactcaa cggtcatacg ctgcaggagt acatgcagga
    55801 aacgcgcggc tatgccttgc aacctgttcc gtccacgcag gcgtcgattc gcggggaaga
    55861 gagtgagcaa atcgagctac tcaatattcg caaagaaacg catgaagaat atgcgctgag
    55921 tcgtccgcgc gggctgcgtg aagcgttgct gatcgtcgcc tccttcctga tgttcttttt
    55981 ctgcctgatt accccggatg tatttgttcc gtggctggca ggcggcgcat tactgctgct
    56041 gggcgcaggt ctgtgggggc tattcgcgcc cccggcaaaa tcctccctgc gggaaattca
    56101 ttgtctgcgc ggtacacccc gtcgttgggg attgtttggc gaaaacgatc aggaacagat
    56161 caacaatatt tcactcggta ttatcgacct ggtctatccc gcacactggc agccatacat
    56221 tgctcaggat ctcggtcaac aaaccgatat tgatatctat ctcgaccgcc acgtagtgcg
    56281 tcaggggcga tatctttcgc tgcatgatga agtaaaaaac tttccgttac agcactggct
    56341 gcgcagtacg attatcgctg cgggctcgct gctggtgctg tttatgctgt tattctggat
    56401 cccgctggat atgccgctga aattcactct ctcatggatg aaaggcgcac agaccattga
    56461 agccaccagc gtaaaacaac tggctgacgc tggcgtgcgg gtaggcgata cattgcgtat
    56521 cagcggtacg ggaatgtgta atattcgcac ttccggaacc tggagcgcga aaaccaattc
    56581 acctttttta ccgtttgact gctcgcagat catctggaat gacgcccgct cattgccatt
    56641 accagaatct gaactggtca acaaagcgac ggcattgact gaagcagtta atcgccagct
    56701 gcaccctaaa ccggaagatg aatctcgcgt cagtgcctca ttacgttcag cgattcaaaa
    56761 atccggcatg gtattgcttg atgattttgg cgacattgta ctgaagacag cggatttatg
    56821 ttctgccaaa gatgactgtg tgcgactgaa aaatgcgctg gtcaatctcg gcaacagtaa
    56881 agactgggac gcgctggtaa aacgcgccaa cgccgggaag ctcgatggcg tgaatgtgtt
    56941 attacgcccg gtgagtgcgg aatcgctgga taacctggtg gcaacctcca ccgcgccgtt
    57001 tatcacgcat gaaacggcgc gagcggcaca atcactaaac agtccggccc ccggcggatt
    57061 cctgattgtc agcgacgaag gcagcgattt tgttgatcag ccctggcctt cggcatcact
    57121 ttacgactat ccgccgcaag aacagtggaa cgctttccag aaactggcac aaatgctgat
    57181 gcatacgccg tttaacgccg aaggtatcgt cacaaaaatc ttcactgacg ccaatggtac
    57241 gcagcatatt ggccttcatc caatcccgga tcgttccggc ctgtggcgct atctcagcac
    57301 cacattgctg ctactgacga tgctgggtag cgccatttac aatggcgtac aggcctggcg
    57361 tcgttaccag cgtcatcgca ctcgcatgat gaagattcag gcctattatg aaagctgcct
    57421 gaacccgcaa ctgatcaccc cttcagaaag ccttatcgaa taacacgttt gcgcggcagg
    57481 ttatgctacc ctgtcgcgca aattgcttca ctctggagat ttccctcatg catatcaaca
    57541 ttgcctggca ggacgtagat accgttctgc tggatatgga cggcacgttg ctcgacctcg
    57601 ccttcgataa ctatttctgg caaaagctgg tgcctgaaac atggggcgcg aaaaacgggg
    57661 ttacgccaca ggaagcgatg gaatatatgc gccagcaata tcacgacgta cagcatacgc
    57721 taaactggta ctgtcttgat tactggagtg agcaactggg tctggatatc tgtgcgatga
    57781 ccaccgagat gggaccgcgc gccgtactgc gtgaagatac cattccgttt cttgaggcac
    57841 tgaaagccag cggtaagcag cgaattttgc tcaccaatgc gcatccgcac aacctggcgg
    57901 taaaacttga gcataccggt ctggacgcac accttgattt attactttcc acccacacat
    57961 ttggttatcc gaaagaggat cagcggttat ggcatgcggt ggccgaagct acgggtctga
    58021 aagctgaaag aacgctgttt attgatgaca gcgaagcgat tctcgatgct gccgcgcaat
    58081 ttggtattcg ttactgcctc ggcgtgacta atcctgattc cgggattgcc gagaaacagt
    58141 atcaacgcca tccgtcactg aatgactacc gccgcctgat cccctcgcta atgtgaagga
    58201 gacgccatga aagaaaaacc tgctgttgag gttcgactgg ataaatggct atgggctgcc
    58261 cgtttttata aaacccgcgc gctggcccgt gaaatgattg aaggcggtaa ggtgcattac
    58321 aacgggcagc gcagcaagcc gagcaaaatc gtcgagctga atgccacgct cactctgcgc
    58381 cagggaaatg acgaacgcac ggtgattgta aaggcgatta ctgaacagcg tcgccccgcc
    58441 agcgaggcag ccttgctgta tgaagagact gcggaaagtg tagagaaacg cgaaaaaatg
    58501 gcgatggcac gtaaacttaa tgccttaacc atgccgcacc cggaccgacg cccggacaaa
    58561 aaagagcgcc gcgacctgtt acgatttaaa cacggcgaca gtgaataact gtcacctgca
    58621 agagagatga ttatgccgca acatgaccaa ttacatcgct atctgtttga aaactttgcc
    58681 gtgcgcggcg aactggtaac cgtttcggaa accctgcaac agatccttga gaaccacgat
    58741 tatccgcagc ccgttaaaaa cgtgctggca gaactgctgg ttgcgaccag cctgttaacc
    58801 gctacgctga agtttgatgg tgatatcacc gtacagctgc agggcgacgg tccgatgaat
    58861 ctggcggtta ttaacggtaa caataaccag caaatgcgcg gtgtggcgcg cgtgcagggc
    58921 gaaattccag aaaatgccga cctgaaaacg ctggtcggca atggttacgt ggtgatcacc
    58981 attaccccga gcgaaggcga acgctatcag ggcgtagttg gtctggaagg tgataccctg
    59041 gcggcctgcc tggaagatta ctttatgcgt tctgaacagc tgccgacgcg cctgtttatt
    59101 cgcaccggcg acgtagacgg caaaccggct gcaggcggta tgttgttgca ggtaatgcct
    59161 gcgcaaaatg cccagcagga cgactttgac cacctggcaa cgctaaccga aaccatcaaa
    59221 accgaagaac tgctgacctt accggcaaac gaagtgttgt ggcgtttgta tcacgaagaa
    59281 gaggtgacgg tttacgatcc gcaggatgtg gagttcaaat gcacctgctc gcgtgaacgt
    59341 tgcgccgatg cactgaaaac gctgcctgat gaagaagttg atagcatcct ggcggaagat
    59401 ggcgaaattg acatgcattg tgattactgc ggtaaccact atctgttcaa tgcgatggat
    59461 attgctgaaa tccgcaacaa cgcgtctccg gcagatccgc aagttcatta atgagtatgt
    59521 ccggcagaga gggctctgcc ggattttaca acttaatggc ctgatgtgct gcgcttacca
    59581 gcgcctatga aataaatcgc aaattatttt ggtcagatac agcgtcgtat ccgataaaag
    59641 cgttgtcact tacttaagtt tatttccagc gtttcaccat ctaccgtcag atcgcgccac
    59701 gctttcggcc tcgtccccag ttttccatcc ttcgcagctg tgagtaattt ttctttcgtt
    59761 atttcgttcg gaagtaaact catatcaagc gcgtcccatt cttttctgtc tgagtcaaag
    59821 ccatagacaa tgactctgtc atcattaaaa gcaaacagga tccgctccgc ctgaccatca
    59881 ctattcagat cttgctcgac cagtacgcag gcgtcttttt caatacaggt
//
LOCUS       DAZKIV010000011        55804 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZKIV010000011 DAZKIV010000000
VERSION     DAZKIV010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 55804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55804
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.011"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..309
                     /locus_tag="ACHOT6_000737"
     CDS             <1..309
                     /locus_tag="ACHOT6_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
                     /protein_id="HFP9946005.1"
                     /translation="HADLQQYLSQSCGAFVCMAAQEVIEQRESNSDSAPYTLLKNYAD
                     RFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIKHSQPKNRASGKRVS
                     "
     gene            704..1334
                     /locus_tag="ACHOT6_000738"
                     /pseudo
     CDS             704..1334
                     /locus_tag="ACHOT6_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1437..1940
                     /gene="yfbM"
                     /locus_tag="ACHOT6_000739"
     CDS             1437..1940
                     /gene="yfbM"
                     /locus_tag="ACHOT6_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFP9946006.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(2007..3464)
                     /gene="nuoN"
                     /locus_tag="ACHOT6_000740"
     CDS             complement(2007..3464)
                     /gene="nuoN"
                     /locus_tag="ACHOT6_000740"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFP9946007.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALLYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3471..5000)
                     /gene="nuoM"
                     /locus_tag="ACHOT6_000741"
     CDS             complement(3471..5000)
                     /gene="nuoM"
                     /locus_tag="ACHOT6_000741"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFP9946008.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(5164..7005)
                     /gene="nuoL"
                     /locus_tag="ACHOT6_000742"
     CDS             complement(5164..7005)
                     /gene="nuoL"
                     /locus_tag="ACHOT6_000742"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFP9946009.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(7002..7304)
                     /gene="nuoK"
                     /locus_tag="ACHOT6_000743"
     CDS             complement(7002..7304)
                     /gene="nuoK"
                     /locus_tag="ACHOT6_000743"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFP9946010.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7301..7855)
                     /gene="nuoJ"
                     /locus_tag="ACHOT6_000744"
     CDS             complement(7301..7855)
                     /gene="nuoJ"
                     /locus_tag="ACHOT6_000744"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFP9946011.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7867..8409)
                     /gene="nuoI"
                     /locus_tag="ACHOT6_000745"
     CDS             complement(7867..8409)
                     /gene="nuoI"
                     /locus_tag="ACHOT6_000745"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFP9946012.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8424..9401)
                     /gene="nuoH"
                     /locus_tag="ACHOT6_000746"
     CDS             complement(8424..9401)
                     /gene="nuoH"
                     /locus_tag="ACHOT6_000746"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFP9946013.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9398..12124)
                     /gene="nuoG"
                     /locus_tag="ACHOT6_000747"
     CDS             complement(9398..12124)
                     /gene="nuoG"
                     /locus_tag="ACHOT6_000747"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFP9946014.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(12177..13514)
                     /gene="nuoF"
                     /locus_tag="ACHOT6_000748"
     CDS             complement(12177..13514)
                     /gene="nuoF"
                     /locus_tag="ACHOT6_000748"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFP9946015.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13511..14011)
                     /gene="nuoE"
                     /locus_tag="ACHOT6_000749"
     CDS             complement(13511..14011)
                     /gene="nuoE"
                     /locus_tag="ACHOT6_000749"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFP9946016.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(14014..15816)
                     /gene="nuoC"
                     /locus_tag="ACHOT6_000750"
     CDS             complement(14014..15816)
                     /gene="nuoC"
                     /locus_tag="ACHOT6_000750"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFP9946017.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15910..16572)
                     /gene="nuoB"
                     /locus_tag="ACHOT6_000751"
     CDS             complement(15910..16572)
                     /gene="nuoB"
                     /locus_tag="ACHOT6_000751"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFP9946018.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16588..17031)
                     /gene="nuoA"
                     /locus_tag="ACHOT6_000752"
     CDS             complement(16588..17031)
                     /gene="nuoA"
                     /locus_tag="ACHOT6_000752"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFP9946019.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17661..18599)
                     /gene="lrhA"
                     /locus_tag="ACHOT6_000753"
     CDS             complement(17661..18599)
                     /gene="lrhA"
                     /locus_tag="ACHOT6_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFP9946020.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19519..20736
                     /gene="alaA"
                     /locus_tag="ACHOT6_000754"
     CDS             19519..20736
                     /gene="alaA"
                     /locus_tag="ACHOT6_000754"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFP9946021.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20820..21419
                     /gene="yfbR"
                     /locus_tag="ACHOT6_000755"
     CDS             20820..21419
                     /gene="yfbR"
                     /locus_tag="ACHOT6_000755"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9946022.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFQLSLDEISQDSPL"
     gene            complement(21478..23310)
                     /gene="yfbS"
                     /locus_tag="ACHOT6_000756"
     CDS             complement(21478..23310)
                     /gene="yfbS"
                     /locus_tag="ACHOT6_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFP9946023.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23397..24047)
                     /gene="hxpA"
                     /locus_tag="ACHOT6_000757"
     CDS             complement(23397..24047)
                     /gene="hxpA"
                     /locus_tag="ACHOT6_000757"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFP9946024.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(24058..24552)
                     /gene="yfbU"
                     /locus_tag="ACHOT6_000758"
     CDS             complement(24058..24552)
                     /gene="yfbU"
                     /locus_tag="ACHOT6_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFP9946025.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24635..25090)
                     /gene="yfbV"
                     /locus_tag="ACHOT6_000759"
     CDS             complement(24635..25090)
                     /gene="yfbV"
                     /locus_tag="ACHOT6_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFP9946026.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25428..26630
                     /gene="ackA"
                     /locus_tag="ACHOT6_000760"
     CDS             25428..26630
                     /gene="ackA"
                     /locus_tag="ACHOT6_000760"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFP9946027.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILTQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26705..28849
                     /gene="pta"
                     /locus_tag="ACHOT6_000761"
     CDS             26705..28849
                     /gene="pta"
                     /locus_tag="ACHOT6_000761"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9946028.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            29039..30559
                     /gene="yfcC"
                     /locus_tag="ACHOT6_000762"
     CDS             29039..30559
                     /gene="yfcC"
                     /locus_tag="ACHOT6_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFP9946029.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30592..31134)
                     /gene="yfcD"
                     /locus_tag="ACHOT6_000763"
     CDS             complement(30592..31134)
                     /gene="yfcD"
                     /locus_tag="ACHOT6_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFP9946030.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(31192..31743)
                     /gene="yfcE"
                     /locus_tag="ACHOT6_000764"
     CDS             complement(31192..31743)
                     /gene="yfcE"
                     /locus_tag="ACHOT6_000764"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFP9946031.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31799..32443)
                     /gene="yfcF"
                     /locus_tag="ACHOT6_000765"
     CDS             complement(31799..32443)
                     /gene="yfcF"
                     /locus_tag="ACHOT6_000765"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFP9946032.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLAIMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32579..33226
                     /gene="yfcG"
                     /locus_tag="ACHOT6_000766"
     CDS             32579..33226
                     /gene="yfcG"
                     /locus_tag="ACHOT6_000766"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFP9946033.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33283..33645
                     /gene="folX"
                     /locus_tag="ACHOT6_000767"
     CDS             33283..33645
                     /gene="folX"
                     /locus_tag="ACHOT6_000767"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFP9946034.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33666..34559
                     /gene="yfcH"
                     /locus_tag="ACHOT6_000768"
     CDS             33666..34559
                     /gene="yfcH"
                     /locus_tag="ACHOT6_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFP9946035.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPPVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34607..35497)
                     /gene="rpnB"
                     /locus_tag="ACHOT6_000769"
                     /pseudo
     CDS             complement(34607..35497)
                     /gene="rpnB"
                     /locus_tag="ACHOT6_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
     gene            complement(35694..36467)
                     /gene="hisP"
                     /locus_tag="ACHOT6_000770"
     CDS             complement(35694..36467)
                     /gene="hisP"
                     /locus_tag="ACHOT6_000770"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFP9946036.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36475..37191)
                     /gene="hisM"
                     /locus_tag="ACHOT6_000771"
     CDS             complement(36475..37191)
                     /gene="hisM"
                     /locus_tag="ACHOT6_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946037.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(37188..37874)
                     /gene="hisQ"
                     /locus_tag="ACHOT6_000772"
     CDS             complement(37188..37874)
                     /gene="hisQ"
                     /locus_tag="ACHOT6_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFP9946038.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37964..38746)
                     /gene="hisJ"
                     /locus_tag="ACHOT6_000773"
     CDS             complement(37964..38746)
                     /gene="hisJ"
                     /locus_tag="ACHOT6_000773"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFP9946039.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38967..39749)
                     /gene="argT"
                     /locus_tag="ACHOT6_000774"
     CDS             complement(38967..39749)
                     /gene="argT"
                     /locus_tag="ACHOT6_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFP9946040.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(40015..40584)
                     /gene="ubiX"
                     /locus_tag="ACHOT6_000775"
     CDS             complement(40015..40584)
                     /gene="ubiX"
                     /locus_tag="ACHOT6_000775"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFP9946041.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40678..42195)
                     /gene="purF"
                     /locus_tag="ACHOT6_000776"
     CDS             complement(40678..42195)
                     /gene="purF"
                     /locus_tag="ACHOT6_000776"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFP9946042.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(42232..42720)
                     /gene="cvpA"
                     /locus_tag="ACHOT6_000777"
     CDS             complement(42232..42720)
                     /gene="cvpA"
                     /locus_tag="ACHOT6_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFP9946043.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42979..43641)
                     /gene="dedD"
                     /locus_tag="ACHOT6_000778"
     CDS             complement(42979..43641)
                     /gene="dedD"
                     /locus_tag="ACHOT6_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFP9946044.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43631..44899)
                     /gene="folC"
                     /locus_tag="ACHOT6_000779"
     CDS             complement(43631..44899)
                     /gene="folC"
                     /locus_tag="ACHOT6_000779"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFP9946045.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTTSFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44969..45883)
                     /gene="accD"
                     /locus_tag="ACHOT6_000780"
     CDS             complement(44969..45883)
                     /gene="accD"
                     /locus_tag="ACHOT6_000780"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFP9946046.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(46039..46698)
                     /gene="dedA"
                     /locus_tag="ACHOT6_000781"
     CDS             complement(46039..46698)
                     /gene="dedA"
                     /locus_tag="ACHOT6_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9946047.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46781..47593)
                     /gene="truA"
                     /locus_tag="ACHOT6_000782"
     CDS             complement(46781..47593)
                     /gene="truA"
                     /locus_tag="ACHOT6_000782"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFP9946048.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47593..48606)
                     /gene="usg"
                     /locus_tag="ACHOT6_000783"
     CDS             complement(47593..48606)
                     /gene="usg"
                     /locus_tag="ACHOT6_000783"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9946049.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48672..49808)
                     /gene="pdxB"
                     /locus_tag="ACHOT6_000784"
     CDS             complement(48672..49808)
                     /gene="pdxB"
                     /locus_tag="ACHOT6_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFP9946050.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49907..50902
                     /gene="flk"
                     /locus_tag="ACHOT6_000785"
     CDS             49907..50902
                     /gene="flk"
                     /locus_tag="ACHOT6_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFP9946051.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50899..52076)
                     /gene="yfcJ"
                     /locus_tag="ACHOT6_000786"
                     /pseudo
     CDS             complement(50899..52076)
                     /gene="yfcJ"
                     /locus_tag="ACHOT6_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(52360..53580)
                     /gene="fabB"
                     /locus_tag="ACHOT6_000787"
     CDS             complement(52360..53580)
                     /gene="fabB"
                     /locus_tag="ACHOT6_000787"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFP9946052.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            53739..55745
                     /gene="mnmC"
                     /locus_tag="ACHOT6_000788"
     CDS             53739..55745
                     /gene="mnmC"
                     /locus_tag="ACHOT6_000788"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFP9946053.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMAKLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT        13926 a        15214 c        13938 g        12726 t
ORIGIN      
        1 catgccgatc ttcagcaata cttaagccaa agttgcggtg cgtttgtgtg catggcagcc
       61 caggaagtga ttgaacaaag ggaaagcaat tctgacagcg ccccctatac gttattaaaa
      121 aactatgctg acagatttaa aaaatattca gcagaagagc agtacgaaat tgattttcaa
      181 catcgactgg taaacagaaa ttgttattta gataaatatg gcgatgcacg aatcaatgct
      241 tcttatacac aattagaaat aaaacactca caacccaaaa atagagcatc cggcaaaaga
      301 gtgagttaac atcattgtta atcaattcga ccagtcagta aatatgctga ctggtcgaac
      361 ataatttgct aaaaagtcat cactgactga tgtcagtccc accatctgcc agttcaatct
      421 acgggtattt ccagcgtatc gcggcacgcg attgctggtc tgttgatgta gtggattgta
      481 gttgctgact tttcttaccc tcagatttct gccccacttg tcaattcaat ctgccaataa
      541 ataacataat tatattttta ttcctcacag aaattccttt ttaaaaatag cgaacccact
      601 gtaaaaaaca ctttttgtat atctctacca agcgtaataa aataaacgct cataattcat
      661 tataaataat aagaaaataa cgccttttat tgataattta catataaaaa aattttcctt
      721 ttgttatatt atttctgttt tcactccctc tgattatttt taccaaccct gggtcactgc
      781 attatcgcca acgccccgac acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct
      841 tacacaaact gtgcatccac gtaatgccga caatattgat aatctgaata ggtccgtaga
      901 gtatataaaa gagtactttg tcagtagcgg tgccagagtt accgcgcagg gcgtccccga
      961 ttggctatta cgactctgcg cctggtagcc aggattaccc ttacccgcaa tgtcctggct
     1021 ttatcccgat cggggggact ttattgcctg gtcggcagaa tacaggatat caacgccgtt
     1081 cgtcaggtaa aaacggcatt gttatcatcc cgggatttat ctgtttattc tatgaatgcc
     1141 ccatggttta ttcccggtat tgatttctct gaccacctga attattggca acacgatatt
     1201 cccgccgtaa tgattactga caccgctttt tatcgtaata aacaatacca cttgcccggt
     1261 gatacagcag acagattgaa ttatcagaaa atggctcaga tggtgttaca accttgttac
     1321 acaacagtaa ataataaaaa tttctcgagg cgggtccaga aacagcgaca tgtacatttt
     1381 attttccctc gtctgtttta agatgcgaaa cataaaattc aatggaggaa aaataaatgg
     1441 gaatgattgg ttattttgca gaaattgact cagaaaagat taaccaactg cttgagtcta
     1501 ctgaaaaacc tttgatggat aatatacatg acacgctttc aggattacgc cgactggata
     1561 ttgataaacg ctgggatttt ttacactttg ggctgacagg cacctctgct tttgatcccg
     1621 cgaagaatga tccgctgagt cgtgccgtgc ttggtgaaca cagcctcgaa gatggcatag
     1681 atggcttcct gggattgacc tggaatcagg agttagccgc gacaatcgac cgactggaat
     1741 cactggatcg aagtgagctg cgtaaacagt tttccattaa gcgactcaat gaaatggaga
     1801 tttatccggg agtgaccttt tcagaagagt tagaggggca actctttgct tcgatcatgc
     1861 ttgatatgga aaagctgatt agcgcgtatc gacgtatgct gcgacaaggg aatcatgcgc
     1921 tgacggttat tgttgggtga tggtgtcaga gcgccaaaaa aaaccgccga acatgtcggc
     1981 ggctttctga cttacaaact aacagattac atcagcggca ttgctaaccg cacaatacta
     2041 atcagcggtt gtggccatac acccagcacc agtaccaaca gtgcagaaat cagcaccacg
     2101 ataccgcccg cgctgtactg ccagtttgac ggtgcatcgc gacccggttg ttccggtgcg
     2161 tgcaggtaca ggctcaccgc cacgcgcagg tagtagtaga ggccgattgc cgaaccgaca
     2221 accacggcac ccaccagcca ccacaagtgt gcctggacac cgaccgccag cacgtagaac
     2281 ttaccgataa agcccagcgt catcgggata ccggccagag acagcatcat caccgtcatc
     2341 actgccgcga ggatcggacg atgccagaac agaccgcggt aagagaacag ggaatcagca
     2401 tccgggccac gatacgggct ggacatcagg ctgaccacgc cgaacgcgcc gaggctgctg
     2461 aacagataac cggccaggta aacccctacc gcttccatcg acatctcgcc agtttgcagc
     2521 gcaatcagcg ccaccagtaa atagccgagg tgagagatag atgagtaacc gagcagacgt
     2581 ttgatattgg tctggctcag cgccatcagg ttaccgaaga tgatggaggc aaaggcgata
     2641 atcgccagca ccacgcgaat tgcttcgctg tcacccaccg gtgcgtagag gaacagacgc
     2701 atcaccacac cgaagatagc gattttgctc gccgtcgcca ggaaagtgga aaccggcgca
     2761 ggcgcgccct ggtatacgtc tggcgtccac aggtggaacg gcaccagaga gagtttgaag
     2821 ccgaggccaa caatcatcag gccgaaacct gccagcaaca gcggctcgtt gagcataccg
     2881 tcgccgaggt ttttacccag cgcgacaaac gacaggtcgc cagactgcgc atacagcagc
     2941 gccataccga acagcaggaa agaagacgct gcggcagaaa ggatggtgta tttgatactg
     3001 gcttccagcg aacgtttctg gcggaaagcg taaccgacca ggccaaacag cggcaaagag
     3061 atcagttcga taccgaggaa cagagacgcc agatggttgg cattcgccag caggatcccg
     3121 cccagcgcgg caattaacac caacaggtag aactcatcct tgttgtcgtt atagccttca
     3181 agccacgggt aggcgaaagt acaggtggcg aggctcgcca acaataccag cccggtgtaa
     3241 agcatggcga aaccatcaac gcgcatcagc ggcgtaacgt ccatagcgcc cgcctggcca
     3301 acaaaccaga gcgaaaccag cgccgcgtta agcccaataa ccgagagcgt agcgttgagg
     3361 aaatgattgc gtcgccacgc aatggagagc atcacaacca ccaccgtcaa gccgacgatc
     3421 agcaacggta gcagtgcgat caggttttgt ggagttattg tcatggcgat ttacggcctt
     3481 gtagtagtaa cggaattaac aaaccactgc tggatattgc caatcgcgga gtgcgaggta
     3541 tccagaatcg gctgcggata gaagcccagc agtaccagca gcaccaccag caacaggatc
     3601 ataaacagct cacgcagcga catccctggc agttcctggc tggcaatctg gcttttcgct
     3661 ttaccgaagt aagcgcgatg taacatcgcc agcgaataaa cagatgcaaa gaccagccca
     3721 aaggtagaga taacggtaat caccggtaca acctggaagc tgccgaacag aatcataaat
     3781 tcgccgacga agttaccggt gccaggcatc ccaagcgttg ccaccgcaaa gaacagcgac
     3841 agtgctggca gccatttcat cttgctccac agaccgccca tcatgcgcat gtcgcgggta
     3901 tggatacgtt cataaagctg accacaaaga ataaacagac ccgccgccga caaaccgtgc
     3961 gcaatcatct ggattaccgc gccctggtag gccaactggc tgccggtgta gatagcaatc
     4021 agcacgaagc ccatgtggga aaccgaggtg taggcgatca gacgtttgat atcggtctgg
     4081 gcgaaggcca tccacgcacc gtagaagatg ccgataacac ccagccacat agcaattggc
     4141 gcgaactctg ccgacgcgtt cgggaacagc ggcagggaga aacgcagcaa accgtaagcg
     4201 gcagttttca gcaagatccc cgcgaggtca acggaaccgg cggtcggagc ctgggagtgc
     4261 gcatccggca gccagccatg cagcggaacc accggcattt tgactgcgaa ggcgatgaag
     4321 aagcccagca ttaacaggta ttccacacca ctggacattg gcgtattcag cagctcttca
     4381 tagttgaagg tccagacgcc ggtcgcattg tagtgaacaa aaaccagcgc caggatggca
     4441 atcaacatca ccaggccact cgcctgggtg taaatgaaga acttggttgc cgccgtgata
     4501 cgcgttttac cgtcagaggc tttatgcccc cacagtgcga tcaggaagta catcggcacc
     4561 agcatcattt cccagaagaa gaagaacagg aacatgtcga tggcaaggaa cacgccgata
     4621 acgccgccca ggatccacat caggttgagg tggaagaagc cctgatattt ttcgatctct
     4681 ttccacgaac atagtaccgc cagcacaccg agcagaccgg tcagcacgac catcagcagc
     4741 gacagcccgt caatggcgag atgaatagag ataccaaaac gcgggatcca cggcatgtcg
     4801 aattcagact gccactgcgg aattccggcg gattgcgtca gtgaataacc gccctgcaac
     4861 cacagttgca gcgacagcgc cagcgtcaat cccatggtga tcagcgcgat ccagcgtggc
     4921 accttgacgc caaagcgttc ggtctgccag cacaggaagc cgccaataaa gggaattaat
     4981 attagccagg gtagtaacat ggcgatcttt attccttgta aaagtcccgt caggaccgga
     5041 ttttcaacga attctcacga cagaaaattc tcttcggact gggggtagtg tcggatgcac
     5101 cgcgaaggcg gcttatccga cctacgttct tatatgccca ggggcaatcc cacaatcctt
     5161 aactcaacgc agtaccatca acagtgccag caccacgacc gcaccgatgc tcatggatgc
     5221 cacataccag cgcagatagc cgttctcact taacagcaga cctttacctg caaagcggga
     5281 aaggacagcc gggatgttca tcattgagtt cagcggatcg cgtttcagca accaggcaat
     5341 acccaggaac ggcttgacga acactttgtc atacagccag tcaaatcccc aggcgttgta
     5401 ccaccaggtg cccagcagac ggcccggcgc actgttggcg atggaggtca ccagagtacg
     5461 tttacccagc cacagccagg ctgccagcag aatgccgacc accgcgacca cgccagaggt
     5521 aatttccagg gtcaacatgc tgccgtgcgc cagttccgtc gtttgcggaa gcacgccctg
     5581 cagcggcggt acaatcagtg cgccaacgaa ggtggaaagg atcagcagca caatcagcgg
     5641 caggctgtga gttacccctt tcacggcgtg agcgtgaatt tgttcttttc cgtggaagac
     5701 gatgaaaatc atacggaagg tgtagagcga ggtcataaac gcaccgacca gacctgccac
     5761 catcagattg atatgaccat tcgccatcgc acccgcgagg atctcatcct tactgaagaa
     5821 gcccgcagtg accagcggta gtgccgacag tgctgcgccg cccaccagga agcagagata
     5881 aaccagcgga atagatttac gcagaccgcc catcttgaag atgttctgtt cgtgatggca
     5941 ggccagaatg acggaaccgg atgccaggaa cagcagcgct ttaaagaacg cgtgagtcat
     6001 caagtggaaa atcgccgcat cccatgcctg cacgccaagc gcgaggaaca tgtagccaat
     6061 ctggctcatg gtagagtaag cgagaacacg tttgatgtcg gtctgtacca gcgcggcaaa
     6121 accggccagc agcagcgtaa ccgccccgac aatacccacc agatgcagaa cttccggcgt
     6181 catcaggaac aggccgtggg tacgggcgat caggtagaca cccgcggtta ccatcgttgc
     6241 ggcgtggatc agcgcggaga caggcgtcgg gcccgccatc gcgtcagcaa gccatgtctg
     6301 caacggcaac tgcgcagatt taccgaccgc accgcccagc agcatcagcg tcgcccacat
     6361 cagcatgtta ttgccgtcag caaagtgcgc tggtgccagt tccaccattt cgcggaagtt
     6421 cagggtgccc agttcgttgt aaagaatgaa cagtgcgaaa gcgaggaaaa cgtcacccac
     6481 acgggtcacg acgaacgctt tcattgccgc tgcgccattc ttcggatcgg tgtaatagaa
     6541 accgatcagc agataggagc acaggcccac gccttcccag ccgaggtaca tcagcagcag
     6601 gttatcggcg agaaccagaa ctaccatgct ggcgatgaac aggttggtgt aggcgaagaa
     6661 gcgagagtag ccctcttcac cgcgcatgta ccaggaggcg tacatgtgaa taaggaaacc
     6721 cacaccagtg accaccgaga gcatggtcag cgacaggccg tccagcacca ggttaaaacc
     6781 gatgttaaag tcgcctaccg acatccacgt ccacagcggc tggctgtatg actgctcgcc
     6841 gttagcgaag aaatcaacgc cgataaaggc ggttaccagc gccgccaggc ccacagagcc
     6901 aacgccgacg atcgccgaaa cgttttcaga ccagcgccca cgggagaacg ccagcaggac
     6961 gaagccaatc aatggcaaaa taatggttaa ggcaagcatg ttcatccgcg catctcactt
     7021 actgaatcga tgttcaggtt ctggcgacga cggtgaagtt gcagcagcag cgcaaggccg
     7081 atactcgctt ctgccgccgc gaggctgatg gcgagaatgt acatcacctg accgtcggtc
     7141 tgaccccagt agcttccggc gaccacaaag gccagcgcgg aggcgttaat catgatttcc
     7201 agaccaatca gcataaacag cagattgcga cggataacca gaccggttaa gccaagaatg
     7261 aataagattg ccgcgaggat cagtccatgt tgtaagggga tcatgcgtgc tcctccgttt
     7321 ttcttttcgc gctgtcgtct ttacgattgc tcagcacttc acccgcacgc tcttcacgac
     7381 cgacgtggaa ggccacaacc agacctgcga gcagcagcat agaagccagt tccaccgcca
     7441 gtacgtaagg cccgaacagc gtaataccca ctgctttagc actgattggc gtaccgtcga
     7501 taccctgatc gttaacaccg aggatggcgt aaacaatcac caccagcatg atggccgaca
     7561 aaattgccgg accaatccac acctgcggtt tcagccactg gcgttcctgt tcaatttctg
     7621 aaccgcccag gttgagcatc atcaccacga acacgaacag caccataata gcacccgcgt
     7681 agacgataat ttccagcgca ccggcgaagt aagcgcccag tgagaagaac accccggaaa
     7741 tcgccagcag cgaaataatc aggtacagca gtgcgtgtac cggattggta tgggtgatca
     7801 ctcgcaaggt cgcaagtatg gctatcaggc cacagatata aaaagcgaac tccattgccc
     7861 ctctccttac ggtaacaggc tcttgacgtc gataggcttg gcttcgttct ctgcttcgcc
     7921 cttatctttg ccgtcgattg ccatacctgc catccggtag aagttatatt ccgggtattt
     7981 gcccggaccg gagatcagca gatcctcttt ttcgtaaacc agatcctggc gcttgtattc
     8041 ccccatttcg aaatccgggg ttaactgaat cgccgtggtc ggacaggctt cttcgcacag
     8101 accacagaaa atgcagcgtg agaagttgat gcggaaaaac tccgggtacc agcgaccgtc
     8161 tttggtttct gctttttgca gcgagataca gccgaccggg caggctaccg cgcagaggtt
     8221 acaggctacg caacgctctt cgccgtccgg gtcgcgggtc agaacgatac gaccacgata
     8281 acggggcggc agatagaccg gctcttccgg gtacattcgc gtttcgcgtt tggcgaacgc
     8341 gtgcaggccg atcatccaga tactacgaac ctgggtgccg aaacctacta acaattcttt
     8401 taaggtcatg gtcttattgc cccttattgc gcctgccaga gaatgacagc cgccgttacc
     8461 agcaagttga tcagcgtcag cggcaggcag attttccagc cgaaggacat tacctggtca
     8521 taacgcggac gcggtaacga cgcacgaatc aaaatgaaca tcatcataaa gaacgcggtt
     8581 ttcagcgcga accagatgaa tggcggtaac aacgggcctt gccagccacc gaagaacagc
     8641 gtcaccatca atgcagagat ggtcacaatc ccgatgtact cacccacgaa gaacagaccg
     8701 aacttcatac cggaatattc aatgtggtaa ccatccgcca gttcctgctc ggcttccggc
     8761 tggtcaaacg ggtgacggtg acataccgcc acgcccgcga tggcaaaggt aataaaacca
     8821 aagaattgcg ggataacgtt ccacacatgc gcctggctgt tgacgatgtc ggtcatgttg
     8881 aatgaaccgg cctgcgccac cacgcccatc agggaaagcc cgaggaacac ttcgtagctc
     8941 agggtctgcg cagaagcacg catcgcaccc agcaacgagt atttgttgtt acttgaccag
     9001 cccgcaaaca gcaccgcgta aaccgccaga cctgccatca tcaggaagaa caaaatcccg
     9061 atgttcaggt cggcaaccac ccaacccgga ctgactggca caatcgcaaa ggccagcagc
     9121 agcgaggtaa aggcaatcat cggtgccagg gtaaagatga cgcgatccga gaatttcggg
     9181 atccagtctt ctttaaagaa cattttgatc atgtccgcaa ccagctggag cgaaccgccc
     9241 cagccaacac ggttaggtcc gtaacggttc tggaacagac ccagcaggcg acgttcgcca
     9301 aagctcatga atgccccgca ggtgacaacc accagcagga tcaccaccgc tttgaggatg
     9361 gtcagcagga tctcaatcag ttccggtgat atccaactca ttgttgtgcc tccttgagat
     9421 cctcaagatg cgcgccagcc agcaccggag caataccgga catacccatc ggcaagccca
     9481 cctgccctgc cgtcagccct tcagcgattt caaccggcag cgtgaccgtg ttgccatcgt
     9541 aactaaagga gacgtgtgta cctgcgttca cacctaactt cgcggcatcc gctgggttga
     9601 gtttgatgta cggctgcggc atacggctct ggaagaccgg agcacgctgt gacaattcat
     9661 cgctgccaaa caggtggtaa tacggcgcga tacgccattt cccgtcctgc ggctggaagc
     9721 gtgccggtac gctggtgaag taatccagac cattttcgct ggtttcaaac agacgcacgc
     9781 ccggatcgcc aaagcgcagt ttgccgccca cttcgtcctg gaatttgttc cacgcctgcg
     9841 gggagttcca gcccggcgcc caggcaaacg gcacttgcga acggtgcgca gtcggctggt
     9901 tgttaccttc catcgagaag gtgaacatgg tgtcaatatc ctgcggctga cgcggctcat
     9961 gaacgctgat attggcgcgc atggcggtac gaccgctgta acggtgcggt tcacgggcca
    10021 gtttctgccc acgaatacgg aatgtcgcat ccggcgcagc atctttgata cctgccagtt
    10081 ccgggatttt cgccacaaca gcgtcaatca catggtcgag ctgcgtccag tccacttcac
    10141 ggctcagcag ggtgctgtgc agcgagtgta accagcgcca gctttccagc atgacagttt
    10201 tgctgtcgta ataagcagga tcgtaaacct ggaagaaacg ttgggcgcgg ccttcgttgt
    10261 tgatcaccgt accgtcgctt tcagcaaagc tggcagcaga aagtaccaga tgggcgtttt
    10321 ccataatcgc tgtgcgttga tgatcaacca ccatcaccag cggcgcttta gccagcgcag
    10381 cattcacgcg ggtagcggaa gcgtgacgat gcagatcgtt ttccagcacc accaccgcgt
    10441 cggcgcgtcc ggtttccagt tcggtcaacg cttcttcaag cgaaccgcca cccataatgc
    10501 ccagccccat gctgttgacg gaacgggcaa tcatggtgat accaacgtca gcgccgcgac
    10561 ctttcagggc tttcgcgacg ttagccgcag cctgaatcac ctctatgcta ccggcgttcg
    10621 tcccggagat aatcaacggt ttcttcgcac ctgccagtgc ctgtacgatg acgtcgattt
    10681 tgctttgcag ctcaggttcg ataccgtcaa ccgctggcgc agagttatcc agcgcatggg
    10741 cgatggcaaa acctaaacgc gcctgatctt caaccggtgc gcggtaagtc cacgccgcga
    10801 tatcatccag acgggtgtca tcaacgttgg taacaaacag cggatgcttc gcacgttgac
    10861 cgatgttgag gattgccgca atctgccagt cagccacttt ctgtgctgcc gccatttcgc
    10921 gcgctttacc tttcacagcc tgacgcactg ccagcgcgac gcgcgcgccg gtctgggtaa
    10981 cgtcttcgcc cagcaccagt accgcatcgt aagattcgat ttcgcgcaga gccggagtat
    11041 aaatgccgcc ttcacgcagc actttcagcg ccagttgcag acgttcctgc tcaccgtgag
    11101 cgataccggt gtagaagttt tcttcgccca ccagttcacg cagcgcaaag ttgctttcca
    11161 cgctggcacg cggagaacca ataccgatca ctttcttcga ctgacgcaga atatctgccg
    11221 cgccctgcat tgcctgttcg gcgttgaggg taatgaaatc atcgccacga cgctgtactg
    11281 gttgacgcgg acgatctttc aggttgacgt aaccgtaacc gaaacgacca cggtcgcaga
    11341 ggaagtagtg gtttaccgta ccgttgtaac ggttttcgat acgacgcagt tcgccgtaac
    11401 gttcaccggg gctgatgtta cagccgatgg aacattgctg gcagatgctc ggcgcaaact
    11461 gcatatccca tttacggttg taacgctcgg agtgcgtttt gtcggtaaat acgccggtcg
    11521 ggcaaatttc gaccaggtta ccggaaaatt cgctttccag cgtgccgtct tccgggcgac
    11581 cgaagtagac gttgtcgtgc gcaccgtaaa cgcccagatc tgtaccgtca gcgtaatctt
    11641 tgtagtaacg cacacagcgg tagcaggcga tgcagcggtt catttcgtga gagatgaatg
    11701 gccccaaatc ctgattacgg tgggtacgtt tggtgaaacg gtagcgacgg aagctgtgtc
    11761 cggtcatcac agtcatatcc tgaagatggc agttaccgcc ctcttcacat accggacagt
    11821 cgtgcgggtg gttggtcatc aaccactcga ccacgctttc acggaactgt ttcgcttctt
    11881 cgtcgtcaat ggaaataaag gtgccatcgg aagccggtgt catacaggac atcaccaggc
    11941 gaccacgcgt gtcttccgcg ttttggtatt gcttcaccgc acactggcgg caagcaccga
    12001 cacttcccag cgccggatgc cagcaaaagt aaggaatatc aaggcccaga gacagacaag
    12061 cttccagcag gttgtccgct ccgttgacct cgtattcttt gccgtctaca tgaattgtag
    12121 ccattagcat gcttccagtt ttctcggtca ggaaccgagc gttaatcgaa attcggttac
    12181 cagcgctctt tcagcaggtt cggctgaatc ccattaatca aatgggtatt gctgaacggc
    12241 tgtttgattc ccgcctcaaa ttcttcgcgg aaatatttga tggcgctctg taacggctcc
    12301 actgcaccag gtgcgtgggc acagaaagtt ttacccgggc ctaagaatcg acacagttgc
    12361 tcaagtgttt cgatatcgcc cggctgacct tcaccacgct ccagcgcacg cagaattttc
    12421 acgctccacg gcagaccgtc gcggcacggc gtacaccagc cgcaggactc acgggcgaaa
    12481 aactcttcca ggttacgcac cagcgacacc atgttgatct catggtcaac cgccatcgcc
    12541 agcgccgtac ccagacggct tcccgcttta ccgatacttt cgaattccat cggcaggtca
    12601 aggtgcgctt cggtcaggaa gtcggtgccc gcgccgcctg gctgccaggc tttgaatttc
    12661 agaccgtcac gcataccacc ggcgtaatct tcgaggatct cgcgcgcggt agtaccaaac
    12721 ggcagttccc acagtcccgg atttttcacc cgaccggaga agcccatcag cttggtgcca
    12781 gcatctttac ttttcgagat gttctgatac cactccacgc cgttagcgag gatcgccgga
    12841 acgttacaca gggtttcgac gttgttgaca caggtcggtt tgccccatac gccggaggtt
    12901 gccgggaagg ggggtttcga gcgtgggtta gcacgacgcc cttccaggga gttgattaac
    12961 gctgtttctt ccccgcagat gtagcgccct gccccggtat ggacgaacag ttcgaagtcg
    13021 aaacctgttc ccataatgtt tttgccaagc aagcccgctt cagtggcttc ggcaatggcg
    13081 cggcgcagat ttactgccgc ttcgatatat tcgccacgca ggaagatgta gccacggtaa
    13141 gctttcagcg caaacgcgga gatgagcata ccttccacca gcaggtgcgg cagttgctcc
    13201 atcagcaggc ggtctttata ggtgcctggc tccatttcat cggcgttaca cagcaggtaa
    13261 cggatgttca tggattcgtc tttcggcatc aggctccatt tcaggccggt agaaaagccc
    13321 gcgccaccgc gccctttcag accagcgtct tttacctgat taacgatttc gtccggagac
    13381 agcccggtca gcgccttacg cgcgccttcg taaccgtttt tgctgcgata ttcgtccagc
    13441 cacactggct gtttgtcatc gcgcagacgc caggtcagcg gatgcgtttc gggagtacgg
    13501 ataatgtttt tcatttatac cgctccagca gttcagggat cgcttccggg gtcagatgcg
    13561 cgtgagtatc ctcatcgatc atcatgtttg gccctttatc acagttcccc aggcagcaag
    13621 ttggcagcag cgtaaagcga ccgtcaaatg tcgtttgccc tggtttgatg tttagctttt
    13681 tctcgagcgc cgcctgaata ccctggtaac cgttgatatg acagaccacg ctgtcgcaat
    13741 aacggatcac atggcggcca accggctggc ggaagatctg gctgtagaac gttgccacac
    13801 cttcgacgtc gcttgccgga atacccagca catcggcgat cgcgtggatc gcaccatccg
    13861 gcacccagcc acgctgcttc tgaacgattt tcagcgcttc aatggacgcc gcacgcgggt
    13921 cttcgtagtg gtgcatctcg tgctcaatcg cttcacgctc tgccgcactc agctcaaaag
    13981 cctcggtttg tggttgttga ttctcgtgca taattagcgg tccacatctg acataacaaa
    14041 atcgatactg cccagataaa caatcaggtc agacaccagg ctgccgcgga tcgccgccgg
    14101 aatttgctgc aaatgcgcat aactcggcgt gcggatacga gtgcgataac tcatggtgct
    14161 accgtcgctg gtcaggtagt aactgttgat ccccttggtc gcctcaatca tctggaaaga
    14221 ttcattggca ggcatcaccg gcccccacga cacttgcagg aagtgggtga tcagggtttc
    14281 gatatgttgc agcgtgcgct ctttcggcgg cggcgtggtc agcgggtgat ccgctttgaa
    14341 cgggccttcc ggcatgttgt tgaggcactg ctcaagaatg cgcagactct ggcgcagctc
    14401 ttccactttc agcattacgc gggtgtagca gtcagaaaca ccaccaccaa ccgggatttc
    14461 aaagtcgaag ttttcatagc cagaataagg acgcgcctta cgcacgtcga agtcgatccc
    14521 ggtagcacgc aggcccgcgc cagtggtgcc ccactccagc gcctctttcg cgccataggc
    14581 ggcaacgccc tgggaacgac ctttcagaat ggtattttgc agcgccgctt tctcgtaaga
    14641 cgccagacgt ttcggcatcc agtcgaggaa ctcacgcagc aggcgatccc agccgcgcgg
    14701 caggtcgtgc gctacgccgc caatacggaa ccacgccggg tgcatacgga aacccgtgat
    14761 cgcttccacc agatcgtaaa ttttctgacg atcggtaaag gcgaagaaca cgggcgtcat
    14821 tgcgccgacg tcctgaataa aggtcgagat gtacagcagg tggctgttga tacggaacag
    14881 ttcggagagc ataacgcgaa tgacgttaac gcgatccggc acggtgatcc cggccagttt
    14941 ctctaccgcc agcacgtaag gcatttcgtt aacgcagccg ccgaggtatt caatacggtc
    15001 ggtatacgga atgtagctgt gccaggactg gcgctcgccc attttctccg caccacggtg
    15061 gtggtagccg atgtctggta cgcagtcgac aatctcttcg ccgtcgagtt gtaaaacgat
    15121 acggaaagcc ccgtgcgccg acgggtggtt cggaccgagg ttgaggaaca tgaagtcctc
    15181 gttttcggtg ccgcgcttca tcccccactc ttccggtttg aaggtcaggg cttccatctc
    15241 cagatcctgt ttggctttgg tcagctcaaa cggcgagaat tcggtagcgc gcgccgggta
    15301 atctttacgc agcgggtgac ctttccaggt ttgcggcatc atgatgcggc gcaggttcgg
    15361 gtgaccgtcg aatgtaatgc caaacagatc ccaggtttca cgctcatacc agttagcgtt
    15421 cgggaacagt ttggtgaagg tcggtacgtg caggtcgttt tctgccagcg ccaccttcag
    15481 catgatgtcg cggttacgat cgatagaaat cagatggtag aaaacggaaa aatccgcggc
    15541 aggtaaccct tcgcggtgtg tgcgcagacg ttcgtccatg ccgtgtaagt caaacagcat
    15601 gacgtaaggt ttcggcagtt tctttaagaa atcgccaact tccagtaatt gttcacgctt
    15661 gatccacaca acgggaaccc cggtgcgagt cgcctgaaca gtaaaggcat ccggcccaaa
    15721 acggttgcgc agttcgccaa tcaccggatc atcaagatga tcgcgggtct gccaggcggg
    15781 ttcttgcgcg gttaagtcgg tcatattgtt caccattgca aatggtccgt ggtgactgtc
    15841 ggcgcagtaa gcttcgcgaa atagatttag gaataagcga agaaaaatcc ctttgccgac
    15901 aggcgcaaat taaatctcgt caggtgtacg caggttggtt acggcaatgc gttcaccgcg
    15961 cttgcgttcg cgctctgatt gcatattggc gcgataaacg ccctgatcgc caaccaccca
    16021 ggagagcggg cgacgttctt tgccgataga ttcctgcaac agcatcagtg cctgcatgta
    16081 cgcttcagga cgcggcgggc agcccgggat atacacatca accgggatga atttatcgac
    16141 gccctgcaca acggaataaa tatcgtacat accgccggag ttggcacagg cccccattga
    16201 gataacccat tttggttcca gcatctggtc atacagacgc tgaataaccg gtgccatttt
    16261 ggtaaagcag gttcctgcca ccaccatcag gtcagcctga cgcggcgaag cacgcaatac
    16321 ttctgcgcca aaacgcgcca cgtcatgcac cgcggtaaac gaagtcacca tctcaacgta
    16381 acagcaggaa agaccgaagt tatacggcca aattgagttt ttacgacccc agttaaccat
    16441 gtcattgagc ttgcccataa acacgttttt gttaacttct tgctccagag ggtcggttac
    16501 gatctcctgc ttttgcaggg ggtaacggtc gttctcaccg ttgggatcta tgcgggtgag
    16561 cgtataatcc atcttaatgc ctcgcggtta gcgttgacga ttagcgatac tgttcgtttc
    16621 cgggttcata cgctcgcggc gtgaacgcgc gggcgtccag tccagcgcgc caatacgcac
    16681 cagataaacc agacctgcca gtaacacaaa aataaaaatt gcagcttcca caaagcctac
    16741 ccagccgctt tcgcggatag aggttgacca tgcgaacaga tacagcgctt caacgtcgaa
    16801 gataacgaag aacatggcca ccagataaaa cttggcggac aggcgtaagc gggcggagcc
    16861 gaccgagtcg ataccggatt caaacggtac gtttttcgac ctcgcgcgtg cgcgaccgcc
    16921 taaaaaccaa ccgcctacca gcatcaggca acacaggcca atggcaacga taagaaagat
    16981 agcgaatgcc cagtgatgag cgatgacttc agtggatgtt gacatactca ttgcttactc
    17041 atcaaaagta gcgccagatt cactgctctt cacggcagat ggacgccaca tcgattcatg
    17101 gggaggaata aaaaaaacct tacaatcact gtagaaattc ttttatacag ctaattgatg
    17161 tggtctttta ctcctttcta taaccttttg tcaactttaa caaaagtttc ttcacattag
    17221 tttacataat atcaacacca ttagcattta atgccctttc accccagaac cttgacgact
    17281 ccaggataat tagatgttgt tgaatcgtgt ccgttgtgaa gcaatggaaa aaatacgggt
    17341 ctattttgac aggaatttgt gtcgattcct ccccccaaaa gagagtattt tcttgatctg
    17401 tgacacgctt ttgtcattcc ataacaaaaa cgcagcaaca aatttacgta ttttttaaca
    17461 tcattgtagc aggtgatttt tttcaggcga ttatttgtgc gtacggggcg tgaatctctg
    17521 gtgattgaaa aatgaacagt tttgtgcgtt ctgcacgata cgaggaaggc tttttactaa
    17581 aaaaaagccg ctggagttta aaatcccccc agcggctcgt ttttacacta ttgttcctgg
    17641 aattatctat cgtccgtcga ttactcgata tccctttcaa ttaacaatga gtcatctcct
    17701 tccggagcag acattgggct gtattgccac ggattatggt agctctccat cgcctgatag
    17761 atcacctggg ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatcg
    17821 ggtagcggcg gtaagccatc tactccactc agcacacgca ggtctggact catcatctcg
    17881 accggccttg ccgtcacgcc aagaccggct tttactgccg cacgaactgc cggaagcgtc
    17941 gaggcgacat aagccagtcg ccagggaata tctgctttat taagcgtcgc cagcaccata
    18001 tcgcgaaacg gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat
    18061 acgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac
    18121 gctgaaggac gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc
    18181 atataggcat tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg
    18241 cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac
    18301 acgccctgaa gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt
    18361 ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga
    18421 cgagcgaaca gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg
    18481 gactgagtac gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca
    18541 acaaatgttc tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata
    18601 tattatcact tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc
    18661 aattccattt gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa
    18721 tgcatatgag ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga
    18781 tcacagaata tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat
    18841 gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc
    18901 gctggtcata cgcgaatacg tacaaacagc aatggtgagg cggcatcaag agcaggggtc
    18961 cgtcatttat gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt
    19021 atctgatgca aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc
    19081 aaaacctttt tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc
    19141 ttaacaatag tttcacacct tgcccccatt gcgacacctc ggaaatcatc acgctgttaa
    19201 actctccgta atgcggaaat atcatcatca cgtttcatta gtgaattctt aaatgaggca
    19261 ttttcacaca attatcttac agataaaaaa accagactta caattaacaa tcagaacaat
    19321 tcactatata acattgcatg taaagcattt acacctaatt attttgtcat tattaagtta
    19381 ttaacagcgc aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca
    19441 ccctcctggg ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag
    19501 caaaaaaggt caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt
    19561 tatgacatcc gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag
    19621 gtactgaaac tgaacatcgg caacccagcc ccgttcggtt ttgacgcgcc agatgaaatc
    19681 ctcgttgacg tgatacgcaa cctgcctaca gctcaagggt attgcgattc caaaggtctt
    19741 tactccgcgc gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc
    19801 gtggaagata tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca
    19861 ttgctgaaca gcggggacga aatgttggtt cctgcaccag attacccact atggaccgcg
    19921 gcggtttcgc tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg
    19981 ttcccggacc tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gatcgttatt
    20041 atcaacccaa ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg
    20101 gagattgcac gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc
    20161 tacgacgatg ctgagcatca ctcaattgcg ccgctggcac ctgacctgct gaccattacc
    20221 tttaacggac tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg
    20281 aacgggccga aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg
    20341 cgcctgtgtg ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag
    20401 agcatcagtg aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg
    20461 gaactgatca acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg
    20521 ttcccgaaaa tcgacgccaa acgctttaac attcatgacg atcagaaaat ggtgctggat
    20581 ttcctgttgc aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg
    20641 gatcacttcc gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag
    20701 ttcgcgcgtt tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg
    20761 catccggcag tgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta
    20821 tgaaacagag ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc
    20881 taatgcgcaa cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg
    20941 cccatgcgct ggcagctatc aaaaatcgga aatttggcgg taatgtcaac gccgaacgta
    21001 tcgctttact ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc
    21061 cggtgaaata cttcaattcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc
    21121 agcagaaact ggtcgatatg gttccggaag aactgcggga tatctttgcg ccgttaattg
    21181 acgagcatgc atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg
    21241 catatctgaa atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa
    21301 cgcgactgga agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag
    21361 tatttgttcc cagcttccaa ctttcgctcg atgagattag ccaggattca ccgctgtaag
    21421 cagccggagt ctgcgtcgca tcaggcaata agcgccggat gcgacatcag gctcttgtca
    21481 aaacggaaac agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg
    21541 tacccccaac ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagtgtgtt
    21601 aacaggtgaa gaaaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc
    21661 gaatggataa ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa
    21721 caccgccgtc gcggtattag agataaatag cccaataacc gccgacaaga caaacaaaca
    21781 ccccagcatc atatgtggcc cgtaaccacc gccaatgtcc atcagccctt tcaccgccag
    21841 cgcgacacct cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat
    21901 aatgctcggc cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc
    21961 catcagcagg caggcgatga tagcggcaac aggattagga atttcatctg tcagcattaa
    22021 cgccaccatt agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc
    22081 ttcactcacc tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat
    22141 accgatcagt ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc
    22201 cgccagcgaa ccttccagcg ccacgccatt gcgctttagc cccaccacat tcagtccgta
    22261 gcgggtacga aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa
    22321 tgaaatctct gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac
    22381 catcggctcc agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat
    22441 atcaataagc aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac
    22501 gcgacgaaaa cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa
    22561 tttgagatca tccagccgtt gaccaatcat tggcgatccg gggcgaatag ccagacgtcg
    22621 cgcacgcccg gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc
    22681 ttcgcgctgc ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa
    22741 aatacccagc accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg
    22801 atagccttca cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt
    22861 catcatgccg cttatcagcc cggcaaaact caacggcatc atcagacgtg acggcgaagt
    22921 ttgcatacgc atggcaacgc ttaacaccac gggaataaag atagcgacaa cgccggttga
    22981 actcataaac gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc
    23041 actgttgccc gcaactttga ccagccatgt tcccattacg gtggcaacac cggtacggac
    23101 caaaccatcg ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc
    23161 ggaaaatact tcggggactg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa
    23221 agcgaccgca tccatacgca ctctgcccgt cgcaaacaag acaatggcaa cggccagtaa
    23281 tgaaagaacc caaatcaatt caccgttcac aacttatcct tgttaattga gggggatgac
    23341 ttgattctgc cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac
    23401 tgaataataa catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg
    23461 accaaatcga cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatgg
    23521 caacccgccg ccaggccaga aagcacgcca gcgggagcat cttccaccac cacacactcc
    23581 tgcggcgcaa gccccagcag ctgcgcacct aacagatacg catcaggttc tggttttccg
    23641 cgcttcactc gctcagcggt tacaaacacc tctggtgcgg gaagcccagc tattttatgg
    23701 cgcgctcgcg ctaccggcat ggagccagaa gtcacaatgg cccacggaat acctgcttta
    23761 tttaaatgat tgagtaaggc gattgccccc ggaagcgcgg taataccttc ggtttccgtg
    23821 gcctcgatgt gctccagacg cgtaaactcg gcggcaatat cagcctcgga tttgcccgcc
    23881 ataaaatggc gcaaagaggt gatcgcctgt ttaccgtgaa tgaaagccag cacctcttcc
    23941 ggcgctaacc catgacgtct ggcccagttg ctccacgccc gttctaccgc aggcagggaa
    24001 tccaccagcg ttccatcaag atcaaacaga aaacctttgc accgcacgcg ggcctcctca
    24061 ggcgttgata atttgattaa tttcgttggc gctcaaatgg tactgacgcg ggcaggcatg
    24121 ccacacatta agcatgcgct gatatttttc ccacattggc gtctgggcgt taaaaccgtg
    24181 agttccggcg tcaaaatggg tatagcgccc ttccacatta accataaagc ggacataacc
    24241 gaggtaacgt gcttcagtgg cggcgtcaaa gccgaggaag gtgacacgac gttcatcgat
    24301 ggattgctgg tcctgcaaat tagaccagga aacatgcaac gcatgataca tctccataat
    24361 gtcgatgata gtgcggcagg tttcttcttt cagctcgcca aactcgcgat ccaattcacg
    24421 catctgtaat ccgtaaccac gctcaataat tgtttgcagg cgacggtaac gttcagcatt
    24481 tgccggatcg agcatagtca tcatcttgta ctgattagac aaaataagac gttgcgcgtt
    24541 ggtcatttcc attgttgact cctgtatcac tatactacgg tgaaaaaaaa gaaggctgag
    24601 tatgccttct tttatatgcg taatcagggg tcaattacaa atcatcaagg aaagttttat
    24661 ccagttgttt gaaggcgcgc ttaagcgtgt cagctaatgc ctggtaatca ggcttgcctt
    24721 caacgggtgc caacacctgt ccagactcct gcaatttacc gcgaacttca taaaaccagt
    24781 taaggattgc agggggtaat ggcgttacag aacgcttgcc cagccaccac aatccctgca
    24841 tgggtaaact taaggcgaac agcgcagtgg caactgccgg cccaagctga ccgcccaggg
    24901 caatctgcca gcagagagta aatacggcga tcggcggcat aaaacggatc gcataacgcg
    24961 tcatcttgat aacgcgattt tcgacaaaga ccggggcaag gcgtttttcc agtggccacg
    25021 tctttgagta atgctgtccg cggcgaaaca agctaaaaaa attaacagaa cgattatccg
    25081 gcgttgacat gcttcacctc aacttcacat ataaagattc aaaaatttgt gcaaattcac
    25141 aactcagcgg gacaacgttc aaaacatttt gtcttccata cccactatca ggtatccttt
    25201 agcagcctga aggcctaagt agtacatatt cattgagtcg tcaaattcat atacattatg
    25261 ccattggctg aaaattacgc aaaatggcat agactcaaga tatttcttcc atcatgcaaa
    25321 aaaaatttgc agtgcatgat gttaatcata aatgtcggtg tcatcatgcg ctacgctcta
    25381 tggctccctg acgttttttt agccacgtat caattatagg tacttccatg tcgagtaagt
    25441 tagtactggt tctgaactgc ggtagttctt cactgaaatt tgccatcatc gatgcagtaa
    25501 atggtgaaga gtacctttct ggtttagccg aatgtttcca cctgcccgaa gcacgtatca
    25561 aatggaaaat ggacggcaat aaacaggaag cggctttagg tgcaggcgcc gctcacagcg
    25621 aagcgctcaa ctttatcgtt aatactattc tgacacaaaa accagaactg tctgcgcagc
    25681 tgactgctat cggtcaccgt atcgtacacg gcggcgaaaa gtataccagc tccgtagtga
    25741 tcgatgagtc tgttattcag ggtatcaaag atgcagcttc ttttgcaccg ctgcacaacc
    25801 cggctcacct gatcggtatc gaagaagctc tgaaatcttt cccacagctg aaagacaaaa
    25861 acgttgctgt atttgacacc gcgttccacc agactatgcc ggaagagtct tacctctacg
    25921 ccctgcctta caacctgtac aaagagcacg gcatccgtcg ttacggcgcg cacggcacca
    25981 gccacttcta tgtaacccag gaagcggcaa aaatgctgaa caaaccggta gaagaactga
    26041 acatcatcac ctgccacctg ggcaacggtg gttccgtttc tgctatccgc aacggtaaat
    26101 gcgttgacac ctctatgggc ctgaccccgc tggaaggtct ggtcatgggt acccgttctg
    26161 gtgatatcga tccggcgatc atcttccacc tgcacgacac cctgggcatg agcgttgacg
    26221 caatcaacaa actgctgacc aaagagtctg gcctgctggg tctgaccgaa gtgaccagcg
    26281 actgccgcta tgttgaagac aactacgcga cgaaagaaga cgcgaagcgc gcaatggacg
    26341 tttactgcca ccgcctggcg aaatacatcg gtgcctacac tgcgctgatg gatggtcgtc
    26401 tggacgctgt tgtattcact ggtggtatcg gtgaaaatgc cgcgatggtt cgtgaactgt
    26461 ctctgggcaa actgggcgtg ctgggctttg aagttgatca tgaacgcaac ctggctgcac
    26521 gtttcggcaa atctggtttc atcaacaaag aaggtacccg tcctgcggtg gttatcccaa
    26581 ccaacgaaga actggttatc gcgcaagacg cgagccgcct gactgcctga tttcacaccg
    26641 ccagctcagc tggcggtgct gttttgtaac ccgccaaatc ggcggtaacg aaagaggata
    26701 aaccgtgtcc cgtattatta tgctgatccc taccggaacc agcgtcggtc tgaccagcgt
    26761 cagccttggc gtgatccgtg caatggaacg caaaggcgtt cgtctgagcg ttttcaaacc
    26821 tatcgctcag ccgcgtaccg gtggcgatgc gcccgatcag actacgacta tcgtgcgtgc
    26881 gaactcttcc accacgacgg ccgctgaacc gctgaaaatg agctacgttg aaggtctgct
    26941 ttccagcaat cagaaagatg tgctgatgga agagatcgtc gcaaactacc acgctaacac
    27001 taaagacgct gaagtcgttc tggttgaagg tctggtcccg acacgtaagc accagtttgc
    27061 ccagtctctg aactacgaaa tcgctaaaac gctgaatgcg gaaatcgtct tcgttatgtc
    27121 tcagggcact gacaccccgg aacagctgaa agagcgtatc gaactgaccc gcaacagctt
    27181 cggcggtgcc aaaaacacca acatcaccgg cgttatcgtt aacaaactga acgcaccggt
    27241 tgatgaacag ggtcgtactc gcccggatct gtccgagatt ttcgacgact cttccaaagc
    27301 taaagtaaac aatgttgatc cggcgaagct gcaagaatcc agcccgctgc cggttctcgg
    27361 cgctgtgccg tggagctttg acctgatcgc gactcgtgcg atcgatatgg ctcgccacct
    27421 gaatgcgacc atcatcaacg aaggcgacat caatactcgc cgcgttaaat ccgtcacttt
    27481 ctgcgcacgc agcattccgc acatgctgga gcacttccgt gccggttctc tgctggtgac
    27541 ttccgcagac cgtcctgacg tgctggtggc cgcttgcctg gcagccatga acggcgtaga
    27601 aatcggtgcc ctgctgctga ctggcggcta cgaaatggac gcgcgcattt ctaaactgtg
    27661 cgaacgtgct ttcgctaccg gcctgccggt atttatggtg aacaccaaca cctggcagac
    27721 ctctctgagc ctgcagagct tcaacctgga agttccggtt gacgatcacg aacgtatcga
    27781 gaaagttcag gaatacgttg ctaactacat caacgctgac tggatcgaat ctctgactgc
    27841 cacttctgag cgcagccgtc gtctgtctcc gcctgcgttc cgttatcagc tgactgaact
    27901 tgcgcgcaaa gcgggcaaac gtatcgtact gccggaaggt gacgaaccgc gtaccgttaa
    27961 agcagccgct atctgtgctg aacgtggtat cgcaacttgc gtactgctgg gtaatccggc
    28021 agagatcaac cgtgttgcag cgtctcaggg tgtagaactg ggtgcaggga ttgaaatcgt
    28081 tgatccagaa gtggttcgcg aaagctatgt tggtcgtctg gtcgaactgc gtaagaacaa
    28141 aggcatgacc gaaaccgttg cccgcgaaca gctggaagac aacgtggtgc tcggtacgct
    28201 gatgctggaa caggatgaag ttgatggtct ggtttccggt gctgttcaca ctaccgcaaa
    28261 caccatccgt ccgccgctgc agctgatcaa aactgcaccg ggcagctccc tggtatcttc
    28321 cgtgttcttc atgctgctgc cggaacaggt ttacgtttac ggtgactgtg cgatcaaccc
    28381 ggatccgacc gctgaacagc tggcagaaat cgcgattcag tccgctgatt ccgctgcggc
    28441 cttcggtatc gaaccgcgcg ttgctatgct ctcctactcc accggtactt ctggtgcagg
    28501 tagcgacgta gaaaaagttc gcgaagcaac tcgtctggcg caggaaaaac gtcctgacct
    28561 gatgatcgac ggtccgctgc agtacgacgc tgcggtaatg gctgacgttg cgaaatccaa
    28621 agcgccgaac tctccggttg caggtcgcgc taccgtgttc atcttcccgg atctgaacac
    28681 cggtaacacc acctacaaag cggtacagcg ttctgccgac ctgatctcca tcgggccgat
    28741 gctgcagggt atgcgcaagc cggttaacga cctgtcccgt ggcgcactgg ttgacgatat
    28801 cgtctacacc atcgcgctga ctgcgattca gtctgcacag cagcagtaat ctcgtcatca
    28861 tccgcagctt tgcgctgcgg atatctgaac cggaaataat cactatttcc ggttttttat
    28921 tctcttaatc tgcattaatc ctttctgatt atcttgctta actgcactgc atcaatgaat
    28981 tgcgccatct cactttgcat acttaccact ttgttttgtg caagggaata tttgcgctat
    29041 gtccgcaatc actgaatcta aaccaaccag aagatgggca atgcccgata cgttggtgat
    29101 tatctttttt gttgctattt taaccagcct tgccacctgg gtagttccgg tggggatgtt
    29161 tgacagtcag gaagtgcagt atcaggttga tggtcaaaca aaaacacgca aagtcgtaga
    29221 tccacactca tttcgcattc tgactaacga agcaggcgaa cctgagtatc accgcgtaca
    29281 gctgttcacg acgggcgatg aacgcccggg cctgatgaac ttcccgtttg aagggttaac
    29341 ctcaggatcg aaatacggga cagctgttgg catcatcatg tttatgctgg tgattggcgg
    29401 cgcgtttggc attgtgatgc gtacaggaac cattgataac ggtatcctgg cgcttattcg
    29461 ccatacccgc gggaatgaaa ttctctttat tcctgcgctg tttattctgt tttcacttgg
    29521 cggcgcggta tttggtatgg gagaagaggc cgtcgccttt gccattatca tcgcaccgct
    29581 aatggtccgg ctgggctatg acagtattac caccgtcctg gtgacctata ttgccacgca
    29641 aatcggtttt gccagttcgt ggatgaaccc gttttgtgtg gtcgttgctc aggggattgc
    29701 cggcgttccg gtgctttctg gctccgggtt gcgcatcgtg gtgtgggtta tcgccactat
    29761 gattggcctg atctttacca tggtgtacgc ctcacgagtg aaaaagaatc ctcttctgtc
    29821 acgcgtgcat gagtccgacc gcttctttcg tgaaaagcag gcggatgttg aacaacgtcc
    29881 gtttaccttt ggtgactggc tggtattgat tgtcctgacc gccgtaatgg tctgggtgat
    29941 ttggggcgtg atcgttaatg cctggtttat tccagaaatt gccagccagt tcttcaccat
    30001 gggtctggtg attggcatca tcggcgttgt tttccgcctt aacggcatga cggttaatac
    30061 catggcttca tcctttaccg aaggggcgcg aatgatgatc gcccctgccc tgctggtggg
    30121 tttcgccaaa ggaattttgc tgctggtcgg taatggtgaa gcgggtgatg ccagcgtgtt
    30181 aaataccatc ctcaacagca ttgccaatgc cattagcggt ctggacaacg cggtcgcggc
    30241 ctggtttatg ttgctcttcc aggcggtatt taatttcttc gtgacgtccg gttctggtca
    30301 ggcggcgtta accatgccgt tactggcacc gcttggcgat ctggtcggtg ttaaccgtca
    30361 ggttaccgtg ctggcattcc agtttggtga tggcttcagt cacatcattt acccaacctc
    30421 agcttcgtta atggcaactc tcggtgtgtg cagggtggac ttccgtaact ggctgaaggt
    30481 gggcgcgacc ctacttggac tgctgtttat tatgtccagc gtcgtagtga tcggcgctca
    30541 gttgatgggc taccactaaa aatgttaaga gccgcaattg cggctctttt ttcattctgc
    30601 cgtttcagtc tctacggctt catttttggc attgcgcttc atccacaacg ccagcgcttt
    30661 cagcgagtct ggagtgaact catcgcagcg tgcggtgatt tcttccggcg tcagccagca
    30721 aacttcactg acttcatctt cctgtagggc gaagggaccg tgagagacgc agctgaacaa
    30781 cgcgccccag acacggcaat ttttatcttc gaaatagaac tgcccgtgct cggcaaaggg
    30841 gacaccggca atgcccaact cttcttccgc ttcgcgacgc gcggattcca gcagttgctc
    30901 atcggcctga accacaccgc ctgcggtcgc atctaacatg ccgggtaaaa agtcttttgt
    30961 ctcggtacga cgctggacca gaattttgcc catgccatca tgcacgacga tgtaagttgc
    31021 acgatgacgc agacactgtg cccgcatttg ttcccggctg gcttgtgcaa tgacttcgtt
    31081 ctcttcattg acaatatcca cccattcagt acttgccaaa cgacgctgtt ccattatcag
    31141 gaaaccttct ttttctggcg ctcttacggc gcattttgag ttgtgggtaa attacggatt
    31201 aatcgcgacc tgcgcaatga tactttgatc attgagtgcg attacactaa gtacgtcatt
    31261 atccagcatg ccataactcg ccggattacc gcctttcgga atactcaccg agccggggtt
    31321 gaagtggaaa atctcccccc gctgttctgc cactggcaga tgggtatgac cgtacaccag
    31381 cacatcgttc tggtttaaag caggtagatt ttccgggcca aaaagatgac catgcgtcaa
    31441 aaacagacgt tgtttttcca gtaatacctg ttgccacggc gcggttatcg ggaaatgcag
    31501 cagcatttga tccacttcgc tgtcgcagtt gccgcgcaca gcgataacct tatgtgccac
    31561 ttcattaagc cgttcagcga ctttggctgg cgcgtaaccc tccggtaaag cattacgcgg
    31621 gccgtgattc aacacgtcgc caaggatcac cagccactgg gcaccgcttt gggcaaacaa
    31681 ctccagaaca cgttccgtcg ccggtaacga cccatgaatg tccgatgcaa acatcagttt
    31741 catcactcac tcctcgtcga aaaaacgtaa gctatgatac tggattctgt cgttgctatc
    31801 agccagattg cttcgccgaa agtgcaataa aacgctggac agacgctcgc tgccactgga
    31861 atgtcgcata atccaccagg cgttctggca cctcatcgcc atgtagtacc aggcggttaa
    31921 tcattatcgc cagatcagta tcagcaatgc accattcacc aaataaattc ggctgaccca
    31981 gtgctaacaa atgttctgcc atcgcgaaca gtttctctgc actggctttt ccctcggccg
    32041 ttagtggcgc ttttttcgcc cccgcaaaga caacatccgt cggacgctct tcgcggatgg
    32101 gcatcagatc gctgcgcagc caggcctgaa tctgtcgtgc acgcgcacga ttttctaaat
    32161 caagcggata aatacgttcc caggtcggtg gcgcaaatcg atcttccaga tactccgcaa
    32221 tggcagaaga ttcactcaac tcaaaatcat cgatttgtaa taacggcaca cggcgtgtct
    32281 gaccgtaacc ttgccacgtc ggctgcaaat gttcaccgct gtcgaggtcg atggtcttga
    32341 tatgaaacga caggcctttt tcctgcaacg ccacccaggc ggataacaca taaggggaga
    32401 aaaagtgggc atctgaccaa agcgtgatag cgggtttact cattatgtcc tcggcaataa
    32461 agggggctgg ctatcaaact atagagcctt tttgtcactg tcacgtgacg aatactcacg
    32521 taagtcgccg cgctttctat acttgtggtg ttcagatgat taaacaaccg gagctgcaat
    32581 gatcgatctc tatttcgccc cgacacccaa tggtcacaaa attacgctgt ttctcgaaga
    32641 agcagagctt gattatcgct tgattaaggt agacctgggg aaaggcggtc agtttcgtcc
    32701 ggaatttttg cgcatttcgc ccaacaacaa aattccggca attgttgatc attctcctgc
    32761 cgatggcggc gaaccgttaa gcctctttga gtctggtgcc attttgttgt atctggctga
    32821 gaaaacagga ctctttttga gtcatgaaac gcgtgagcgc gccgccacat tacagtggtt
    32881 attctggcag gtaggcggac tggggccgat gcttgggcaa aatcatcatt ttaatcacgc
    32941 agccccccaa accattcctt acgctattga acgttatcag gttgaaactc agcgtcttta
    33001 ccatgtactg aacaagcggc tggaaaactc gccctggctg ggaggcgaga actacagcat
    33061 tgcggatatt gcctgctggc cgtgggttaa tgcctggact cgccagcgaa ttgacctcgc
    33121 aatgtatccg gcagtcaaga actggcatga gcggatccgt tcgcgccctg ccaccgggca
    33181 ggcactgcta aaagcacaac tcggtgatga gcgttcggat agttaaccga aacaggttct
    33241 cgtgtattat ttcatcctaa gtaaaacaac ggagaacctg caatggcaca acctgccgct
    33301 attattcgta taaagaacct tcgtttgcgt acgtttatcg gaattaagga agaagaaatt
    33361 aacaaccgtc aggatattgt tatcaatgtg acgatccact accccgccga taaagcgcgc
    33421 actagcgaag atatcaacga tgcgctgaat tatcgcaccg taacgaaaaa cattattcag
    33481 catgtagaga ataaccgttt ctctttgctg gaaaaattaa ctcaggatgt gctcgatatc
    33541 gcacgtgaac atcactgggt gacgtatgct gaagtggaga tcgataaact acacgcgctg
    33601 cgctacgccg attcggtatc catgaccttg agctggcagc gttaaccgcc atatcgggag
    33661 gctgcatgaa tatagtgatc accggaggga caggattaat tggtcgccat ttaatcccgc
    33721 gtttgctgga gttgggccat caaatcacgg tagtgacgcg taacccgcag aaagccagtt
    33781 ccgttctcgg ccctcgggtg acactatggc aagggcttgc cgatcaaagc aacctcaacg
    33841 gcgttgatgc ggtaatcaac ctggccggag aaccgattgc tgataaacgc tggactcacg
    33901 agcaaaaaga gcgtctctgc caaagccgct ggaatatcac gcaaaaactg gtcgatttga
    33961 ttaatgccag cgacacgcca ccgccggtac tcatttccgg ctcggcaacg ggctattatg
    34021 gcgacttagg tgaagtggtg gttaccgaag aggaaccgcc gcataacgaa tttacccata
    34081 aactctgcgc ccgctgggaa gaaattgcct gccgggcgca aagtgacaaa acgcgagtgt
    34141 gcctgctgcg taccggtgta gtgctggcac cggatggcgg tattctcggt aaaatgctgc
    34201 cgccgtttcg tcttggcctg ggcgggccga ttggctctgg tcggcagtat ctggcctgga
    34261 ttcatattga tgatatggtc aacggtattc tctggctgct ggataacgac ctgcgcgggc
    34321 catttaatat ggtttcgccc tacccggtac gcaatgaaca atttgcccat gcgctcggtc
    34381 aagcgctgca tcgcccggcc attttgcgcg tccccgcaac cgccattcgg ctgttaatgg
    34441 gcgaatcttc aatactggta ttaggtggac aacgcgcgct gcctaaacgg ctggaagaag
    34501 cgggttttgc gtttcgctgg tacgatttag aagaggcgct ggcggatgtc gttcgctgat
    34561 gtggtttaca gcaaacatcc gccagttaac tcccggtgtt acaggattag tggctttgcg
    34621 cgataagatc gtctggtgaa agtcgggtca ccatcataac taactctctg tctaaacctc
    34681 tatccagcat ctcctgagca atacgcaggg cttcttcgtg tttgccctgc attgcgcctt
    34741 cttcacgtaa tctgtcagca atggtcatca gtttctcctt ttcttgtggt gcgcgttccg
    34801 ctatctcacc aataaatgca cgaaaacgct gggcatcccc tgtttgtaat acgtaattaa
    34861 acagggcttt tagctgtctg tcattagtgt tccctgtaac tagcagcgaa acaatttggt
    34921 cgactaatcc caacagatcg cgctgacgaa tatgtttctg aattaactcc aacagcgcca
    34981 ttttgcggtg ttgcataatc tcgtcatccg gcaccacggt aatatccacc aacggaaacg
    35041 ctgatgaata tattttgcgg gctatagcag gctcggcaaa ttcatcaagc cagcagagtg
    35101 aataaggata aggacttctg caaccatgat aaaacagcat cgggctcacc aatggaagct
    35161 ctttatagcc cgcatcaaga tggttttgca ttgccgcaat ggaataacgc atcatgcgaa
    35221 aagccattaa ttcttccggc ttactttggt gctctatcac tacataaata taacccactc
    35281 cctcctgcgt tttcacagac cacaagaggt cggaataata ttgccgcagg tcgtcatcaa
    35341 taaaactgtt tggttccagt ttaagcgtcg ttaaatcaca cagtttgcgc agcggcgcgg
    35401 gaagatgaat atcaataaaa tcccgcgcgg tgtctggatg gcgtcaaaaa gatttaaata
    35461 ccgcatcatg cggcgtggaa gttgtcgata ttgtcatggc gttccgtcac ccctcaaatt
    35521 taagatgacg cgacaataac cacgattaac acccaatgca gcaggcagtt tttatctttg
    35581 gacagcgaat tcagaagtat ttttagcgga ctgcaacttt tacatttcag cggaagctga
    35641 ctcgcaaatt ccccatgaaa atactccggc agcgttagcc accggagagg aaattatttc
    35701 agcgatccct taaggaactg ttgcagacga gggctttgcg ggttgccaaa taactgctcc
    35761 ggcgcgccct cttcttctat tttcccctga tggaggaaaa tgacatgagt agaaacatgg
    35821 cgagcaaagc ccatttcgtg agtcactacc accatggttt tcccctcttc tgccagttgc
    35881 tgcataatac gcaacacttc gcctaccagt tcaggatcga gcgccgaggt aggttcatca
    35941 aacagtaaaa cttccggttc catcgccagc gcccgcgcga tagaaacacg ctgttgctga
    36001 ccgccggaaa gatgcaccgg atatttcccc tgcgcacgtt cgtctatccc gacttttgcc
    36061 agatacttca ccgcccgctc gcgcgcttcc tgcttgctca ggcccaacac ctgaatcggc
    36121 gcttccatga cgttttccag caccgtcata tggctccaga gattgaagtg ctggaatacc
    36181 atcgtcaggc gtgtgcgcag taagcgcagt tgatttttat cggcgacttt gagttgaccg
    36241 tctttgtcgc gcaccagatt gatcgtctgg ccattgacca cgatcgaccc ttcactcggt
    36301 ttttcgagga agttaatgca gcgcagaaag gtacttttcc ccgatcccga cgatccgatg
    36361 atgcttatta catctccggc attcgcttgc agtgagaccc ctttcagcac ttcatgttcg
    36421 ccgtagcgtt tgtgcaaatc gataacgttt aatttattct cggacatcgt attctcagtg
    36481 cgttgaagaa ggtttcacat gctgcaacca gcgtttttcc gctctgcgaa agaggctgat
    36541 caggacataa gagatgatta aatagagcac cgcggcaatg ccgaaggcgg taaaaggttg
    36601 atacgtggcg gcgttaatat cgcgggctat tttcagcaga tccggcacag tggccgtaaa
    36661 cgccagcgca gtagagtgca gcatcaggat cacttcgttg ctgtatgccg gtaacgcaat
    36721 acgcagcgca gaaggcaaaa taatgcaacg atacatttta aaagtcgaga agccataggc
    36781 tctggcggct tcaatttccc catgcggaac cgaacggatt gccccagcaa aaatctcggt
    36841 agtgtaagcg caggtgttaa gcgtcagcgc cagcacggta cagttcaggc cactgcggaa
    36901 gaaagcgtta aggaattcgg ttcccttaac aatctcaagc gtgtacatgc cggaatagaa
    36961 caccagcaac tgaacataca gcggcgtacc gcgaaaaata taggtaaata accagattgg
    37021 aaactggatg tatttattac tggagacacg accaatcgcc agaaacagcg ccaggactcc
    37081 gcctatcact accgacaaaa taagcagcca cagagtgatc gccacaccag taaagcgata
    37141 accgtcggtc cacagcagcg gtttccagta ttcatgtaag atttcgatca caggtcagcc
    37201 ctcttcacac ccacggagta gcggcgctca aggaacagca gcacaccatt ggaaacagtg
    37261 gtgaaaacca ggtaaatcac gccacagacg atggcgaaat agaacggttc ccaggtactt
    37321 ttgcctgcca gttgggtggc tttgaccaca tcttccaggc cgagtaacga aaccagtgcg
    37381 gtagatttga ggatcacctg ccagttgttg ccaatgcctg gcagggcgta acgcatcatc
    37441 gccggaaaca tgatccgccg aaacacttgc ccacgagtaa aaccgaacgc cgtcgccgcc
    37501 tctatatgcc ctttcggcac tgccataaac gcgccacgga acgtttcggt aaaataagca
    37561 ccgtagataa aaccgagagt aataatacca gcgaccatcg gatcgatatc aatctgcccg
    37621 acgcccatcg cctccgtcac cgtgtttagt gcaatctgca aaccgtagaa aatcagcagc
    37681 atcaacacca agtccggcac gcctcgaatc agcgtggtgt acccttcgaa aatcagcccc
    37741 gaaagccgat tttgcgagag cttaccgcca gcgccaatta aaccgatgat tacagcgagc
    37801 actacagaac tgatagccag ctccagcgtg acgagcgcac cctgtaaaat aacacctgaa
    37861 aacccataca acatgctgcc tgtcctgtcg tgtgtggtga attaccgcct cttgtctccc
    37921 tccggttacc cgaagggagc gggctttctt tcaccgatgg cgattaaccg ccataaacat
    37981 caaaatcgaa gtactttttc gctaatttct cgtaagtacc gtcagcgcgc atttcggcaa
    38041 aggctttgtt cagtgcctcg cgcagttcgt tatcttcttt acgcaggccc atgccggtcc
    38101 ctacgccaaa cagtttttca tctttaacag acgggccacc gaatttgtaa tctttaccga
    38161 caggttgttt gaggaaacct tcgctggcag cgacctcatc ctggaacgcg gcatcaatac
    38221 gtccggcagt caggtcagaa taaatgttgt cctgcccctg atacgagacg atttcaatgc
    38281 cttttggtgc ccaatgttca ttaccgaacg tctcctgggt ggtgccctgc aatacgccta
    38341 cccgtttgcc tttcagcgac tcgactgtcg gctgaatgtc agaatttttc gccaccacca
    38401 aacgagaatc ggcagcgtac agtttgtcgg tgaaggctat ttcttgctga cgtttttccg
    38461 taatggaaag cgatgacatg atggcgtcaa tcttcttcgc ttttaaggac gggattaacg
    38521 catccagcgg attttcgaca aacgtacatt gcgtattgat gcgtttgcat aattcctttg
    38581 ccagatcgat atcgaagcca accagttcgc cttgtgaatt ttttgattca aatggcgcat
    38641 aggtcgggtc ggtaccgatg cggatgtttt gcggaatcgc agcaaacgcc gcagttgcgc
    38701 tggagaaggc cagaaccaga gagagcgata gcaccagttt tttcataatg tcctcaactg
    38761 acagtctttt tatagggaat atttacaggt ttgttacact tatcgtgcca taaaattgac
    38821 catcaaggca aaatatcccg ccccgttaga cgtattattt ttcattaaaa atgcttaagt
    38881 atgcatttca atgcaccacg atagtgcaac aaacctggcg tgcaccatga tgatcattcc
    38941 atcaggtaca gcttcccagc gacgtatcag tcaccgtaga cattaaagtc gaaatacttt
    39001 ttcgccatct tgtcgtaggt gccgtcctga cgcagctcgc caagcgcctt attgaaggca
    39061 gccgtcagtt cagcatcatc tttacgtagc cctacaccgg tgccatcacc gaagtatttt
    39121 ttgtctttta ctgatgagcc agcaaaggcg aaatctttac cagcaggttg cttgaggaat
    39181 ccttcgctgg cagcaacttc atcttgtaac gcagcatcca gacgtcctgc agccagatcg
    39241 gaatagacca aatcctggtt ggcataggcc accacatcca cgcctttgct acgccaggtc
    39301 tcgttagcgt aagcttcctg ggttgatccc tgcagcacac caacatgttt acctttcagt
    39361 gaatccagcg ttggctgaat cggtgaacct ttggcagcaa tcaaacgaga atctgcggcg
    39421 tacagcttgt cggagaaggc aatctcctgc tgacgtttat cggtaatgga aagcgacgaa
    39481 ataatggcgt cgattttttt cgctttcagt gaggggatca gcgcgtcaaa gtcactggca
    39541 acccaggtac atttcacctg catccgtttg cacatctcgt taccgagatc gatatcaaag
    39601 ccaacaaaat cacctttagc atctttcgac gagaacggcg cataggtggt atcggttccg
    39661 atacgtaccg tctccggtag cgccgcatag ctggaagccg ctgcggagag accgactaac
    39721 aaagacagag cgagaatcga cttcttcata cataaccctc aagtgaaaat ggctttttta
    39781 tgttgtgtat tgtgttgtgt gtttgcaggc tctttcatgc aggtcttatg ccatcttgac
    39841 gacaacagta acattcaacg ttaaatatgt taataagacg ttgcattatt gtcctgaagt
    39901 tgaagatagc aggtatagcg gttgaatcgc agcgtttcga ttgaatggca gaaacaaaat
    39961 gtcgaggatt tgatcgcggt tgcaaaattg ccctgaaaca gggcaacagc ggagttatgc
    40021 gccctgccag cgggcaaaga gatcttcagg aagggttatt gcaaactggt caagaacacg
    40081 attaaccgtc tgatttatca catcatcaag ggattgcggg cgatgataaa acgccggaac
    40141 gggaggcata atcaccgcac cgatttctgc cgcctgagtc attaaacgca gatggcctaa
    40201 gtgcaatggt gtttcacgca cgcagagcac caacgggcga cgctctttca gcaccacatc
    40261 tgccgcacgg gtcagtaagc catcagtata gctatggaca atgccggaaa gggttttgat
    40321 tgaacagggt aaaatcacca tccccagcgt ctggaaagaa ccggaagaga tgctggcgga
    40381 aatatcgcgc gcatcgtgcg tgacatcagc taatgcctgc acttcgcgca gggaaaaatc
    40441 cgtttcgagg gataaggtct ggcgcgccgc ctggctcatc accagatgcg tttcgatatc
    40501 tgtgacatcg cgcagaacct gtaataagcg cacgccataa atcgcgccgc tggcaccgct
    40561 gatgcctaca atgagtcgtt tcataaatag ttgccctgtc agacttgcag gcagactttg
    40621 cagtatttcg gcgggagttg caagtcaggg tgccagacgg caccctcagc gaaggcatca
    40681 tccttcgtta tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc
    40741 atcattacgt aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta
    40801 gacgccgttg aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc
    40861 gtcgatcaga tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat
    40921 ttcatcaact tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata
    40981 aacgttcggg aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc
    41041 ttcgcgtgcc atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac
    41101 cagcaggacg tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga
    41161 cttacgacgc agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac
    41221 gaagccctgg cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga
    41281 ggtttctggg atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt
    41341 ctcgcccagt ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgatgaa
    41401 cgagtccggg cgagcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc
    41461 agcacattga cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc
    41521 gacgtcacgc aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat
    41581 atattctgta cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt
    41641 tggatcgcgg aaagcaacca taccgtggcc gataatcatc gccacacagg catatgcgcc
    41701 gcggattaag cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta
    41761 gtggcggaag ttgtccagct cgctagcgaa gatattaagc agaatttccg agtcggaagt
    41821 ggtgttgatg tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt
    41881 cagattgccg ttgtgggcaa gcgtaatgcc atacggagag ttaacgtaaa acggctgcgc
    41941 ttcagaggcg ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc
    42001 ctgcaaacgc tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg
    42061 caaacggaag cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg
    42121 ctgaagcacc gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc
    42181 gacaataccg cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct
    42241 gggcaagaaa cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa
    42301 ttgcgggatc agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc
    42361 gagaaagaag agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc
    42421 cagcacccga tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt
    42481 cacgatagca ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg
    42541 aaccagttcg tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc
    42601 aacaaagaaa gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat
    42661 caggctaacc agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat
    42721 atgtgtccca cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga
    42781 aaacgtttgc gtagggattt ccttcccacg catcaataaa aatggcgctg aaaaaatatt
    42841 caacgccatc gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta
    42901 aacgccttat ccgacctacg gttctaacac tgcccaggcc tgataagacg cgccagcgtc
    42961 gcatcaggca agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga
    43021 aagttgcttc aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac
    43081 cagaatacgg gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc
    43141 ggcaccgcgc agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag
    43201 ttgcacaaca taggctttac ccgtcggtgc agctttttct tccacgaccg gcttcggttc
    43261 tggcttcggc gcaggtggtg cttcaacctt cggtttaggc ggctccaccg gtttcggctt
    43321 cggtggggcg accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc
    43381 cggatcgagc gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg
    43441 cggctgcgtc ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc
    43501 cgcttttgga accagcggga tagccgcaaa ctcatcctga taatgttttt tctgcccgtc
    43561 cagcagccct ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg
    43621 attctgaaac ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga
    43681 ccgtgtggaa agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg
    43741 catcccatgc ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt
    43801 gttctgccgt ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc
    43861 ttttcaacca ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca
    43921 gcacgcgccc gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg
    43981 gattatgcgc gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg
    44041 gcaaaattgc gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg
    44101 cacgcagtgc cgccagcgct gttgcggcat tcggttgcgg gacaagcggc aacggcagat
    44161 tttccagcgt gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc
    44221 actcaacgcc ccgacgttgt agcagtgcac ctttttcctg cgccacatca gcaatggtag
    44281 aaggcatttc cggctcaccg acaattgctg gtttttcgct gcggaagatg cctgctttct
    44341 cgcgaccaat actttcacga tctggcccca gccagtcggt atgatccagc gcaatactgg
    44401 ttactaccgc gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta
    44461 cttccagaat caccacgtca agttgtgcct gtttgaacag ccacaacgcc gacagcgtac
    44521 cgtactcgaa ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg
    44581 tggtgtgggc cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacgca
    44641 ccagatgagg cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca
    44701 gcgtacggca ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg
    44761 gtttcaggac gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag
    44821 ttttactgtg caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag
    44881 gagtgcgttt gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca
    44941 gagccagtgg ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg
    45001 gtaccactac gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg
    45061 ccagaatgct cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatggcgc
    45121 ctttctcgat caggaattca ctgcgctgga atccaggcgg cagtttttcg cgaacggtct
    45181 gttcgataac acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat
    45241 cgcccagcat ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga
    45301 tgtacggcaa gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca
    45361 tcagcgacat cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg
    45421 ggcagttatc ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc
    45481 ccattgaacc gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca
    45541 gagtgccttt catcaccacc agcgcatctt tttcgccagt ttctttctgc gcagatgcca
    45601 gacggtcttt atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc
    45661 tacccagctc cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca
    45721 tacgcatgtg atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt
    45781 ataaaacctg accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc
    45841 gggtgggagt aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct
    45901 gaacctggtt cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac
    45961 agaccatgaa tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc
    46021 gaaccgcgga gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat
    46081 ttcgatgaca cccggcaata tagaaaccac aataatcccg acgatcagca gcttaaggtt
    46141 atcctgaacc atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca
    46201 caataatgcg ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc
    46261 cgcaacaaac ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt
    46321 gccgccatgt ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt
    46381 cgaattggga ttactgaaca acttttcacc gaacaaccgc ccgatagtgt aattgaccgc
    46441 gtcacccaca atcgcggcga tcagcatcag caccaccatc atatggacgt tgagatcgtt
    46501 ggtttccagt gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg
    46561 cgttaccacc agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac
    46621 gccgtactcc gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag
    46681 gaaataaatc aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact
    46741 acgttggcta cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg
    46801 ggcccattgg cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca
    46861 gcccttccgc ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg
    46921 ctatccagct ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc
    46981 tgaccatatg atgtacaaaa gcattcgctt taatatctac caccacataa ggaccgtgac
    47041 gcgtgacatt aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac
    47101 ggaacgaggt gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag
    47161 cgtccagcgg ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat
    47221 gattgtagat gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat
    47281 caggtacagc tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca
    47341 aggtccacgc cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac
    47401 cgtgtacccc tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct
    47461 gggagagcgc cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc
    47521 cgtaatactt actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt
    47581 gctggtcgga cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat
    47641 cagcgcgccg ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat
    47701 accgtagtca ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac
    47761 ctgagtcggg aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc
    47821 acgcgcttct tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc
    47881 gtagaatacc ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat
    47941 tttgcgtact tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa
    48001 caacggcagc atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat
    48061 gccgttgagc aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc
    48121 ggaggctgaa atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg
    48181 tttcagtgcc gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt
    48241 gcggtaatcg gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc
    48301 gagagcaaac agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac
    48361 ccaggcagcg gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc
    48421 gaattcagcg gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc
    48481 gctttcgtta cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt
    48541 ttcaagcagg gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc
    48601 agacatggtg gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca
    48661 tgaagaagag attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat
    48721 gatgccgcac tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga
    48781 tagtttttgc gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg
    48841 tcatcgcggc gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc
    48901 gggccatgca gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg
    48961 tgctgttcat gcccaataaa cttgctatag gcttcaaaca cttgcgtagt accgcgtgct
    49021 ttaccttcca gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat
    49081 tccacgttaa gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct
    49141 tcattcaggc aggtcagcaa cgcagtatta tcgacgactg cgccacggca tgcgttaatc
    49201 agaatcgctc ccggcttcag gctacgaatc agtttttcat ccgccagatg cagcgttttg
    49261 tacggcccgt ctttgaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaac
    49321 tcatccagcg agcggaaatc accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc
    49381 aaggttttaa tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg
    49441 cccacgatcc ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt
    49501 agtgaggaga aaacatattc caccaccgca atcgcattac agccaggtgc cgcggaaaaa
    49561 ccaattcccg cctgctttaa ccatgcttca tcgacatggt cggtccccgc tgtggcagta
    49621 ccaacaaatt taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc
    49681 accatcagcg catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg
    49741 gtcacctcac ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg
    49801 attttcacgt ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc
    49861 tcgcggccag gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat
    49921 ttccggccct cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg
    49981 caatcagcct ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac
    50041 ctcactgacg caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct
    50101 gtcgggagat tcaccgctgc tttcgcgtca cttccctgcc gctgaacaca acctggcgca
    50161 acgtctgctg gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga
    50221 gtatttacgt ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg
    50281 tcagacgccg ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca
    50341 ggaagggaag atggttattc cgcaaccgca gcagcgcgag gcgaccgacc gtcctttatt
    50401 accggcggag cacaatgcgc ttaaacagct ggtgaccaaa cttgcggcgg caacggggga
    50461 acccagcaaa cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt
    50521 aattccagcg aaactgttta accatctggt gacctggctg caggcgcgtc agacgctaag
    50581 ccagcaaaat acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc
    50641 cagtgaactg gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc
    50701 atcgctttct tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa
    50761 agggattgat ccgcgtgata tgcaaccgct gcttaatcct tttccaccaa tgatggacac
    50821 gttgcaaaat atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat
    50881 gctggtctgg ctggttcgtt aaacccgacg aaatgacagt atcgtgacaa taattcccag
    50941 caccgcagag atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat
    51001 tcccgccagc ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag
    51061 tgcggtgccg cgaacttgtg aggggacgcg tttaaccacc tccacgccca gagcaggaaa
    51121 gataagcgaa catccggctc cggttaacgc cgcgcccgct aatgcgaccc acgcacctgg
    51181 ggcttgccag agcaacaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt
    51241 cacgccgcca aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc
    51301 gccaaacgcg gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga
    51361 aacgaaagtc ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt
    51421 ccagataaac ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt
    51481 gcgcactgtg ccgttacagg cccacccagt aagggtaata ccattgtggt gatcgccagt
    51541 gcggcaaaac cgtaatggct atgaatcaac aggccaagcg gcgcaccaac agcgagggca
    51601 ccgtaaatcg ccattccatt ccatgacatc actttgccag agtgttttgc ccctacgatg
    51661 cctaaccccc aggtcagagc gcctgtcagt aactggcttt caccaaagcc aagaatcaaa
    51721 cgcccgatga ccaacagggc aaatttgaac ggtgcggaga caggcaaaat cgccgccagc
    51781 agcaacgcgc cgccagccag accacaagct aacatcccct gaagcgccga acgttttgcg
    51841 ccatattgat cggccagtcg cccggcatag ccacgcgtca gcaccgtagc cagaaactga
    51901 atcccgacgg caatcccgac catggtattg ccatagccca gatcatgatg aacaaacagc
    51961 gggataaccg gcaacggcag ccctacggtc atgtaggtga gaaaaaccgc aaaagcgatg
    52021 cggaagagcg aaaaattggc agaagatcgt gtttcggttt ggcttacagc agtcatgcat
    52081 tactccagaa tgcagcgcaa ggcgaggagt atccccgtct catctctctg gtttcagggt
    52141 tacagtgcgt tggcaggatt taacgcgtac gtcttttcag aaggaaatcg acaaagcggg
    52201 aagtttgcct ggaactggcg gcgattgtca atgatgtgaa aaagggaacc atcaggttcc
    52261 cttttgcgtt agtgccggag gccgcattgg cgcgtaacgt cggatgcgac gctggcgcgt
    52321 cttatccgac ctactccgac tactccgacc tactgcgaat taatctttca gcttgcgcat
    52381 taccagcgtg gcgttggtgc cgccgaagcc gaagctgtta gacataacgg tggtcagttc
    52441 gcgatcggtc gtttcggtca cgatgttcag acctgcagcc tgctcgtcca gctcttcaat
    52501 gttgatgctc ggggcgataa agccgtgttc cagcatcagc agagagtaga tagcttcctg
    52561 tacgccagca gcgcccagag agtgaccggt catggctttg gttgcagaaa tcgccgggct
    52621 cttatcgccg aacacttcac ggatagctgc cagttctttc acgtcgccaa ccggagtcga
    52681 agtaccatga gagttcaggt aatcgattgg ggtatcaacg ccgtgcatcg ccatcttcat
    52741 gcagcgtact gcgccttcgc cagacggagc aaccatgtct gcaccatcag aggttgcgcc
    52801 gtagccaacg atttcagcat agatgtgagc accacgcgcc agcgcgtgtt ccagctcttc
    52861 aaccactacc ataccgccgc cgccagcgat aacgaaaccg tcacggtgag cgtcgtaagt
    52921 acgggaggct ttttccggtg tgtcgttgta tttagtagac agcgcaccca tcgcgtcgaa
    52981 ttcgcaggcc atttcccagc acagctcttc gccgccgcca gcaaacacga tgtcctgttt
    53041 gcccagttgg atctgctcta ctgcgttacc gatacagtgt gcggaagtcg cacacgcgga
    53101 gctgatggag tagttaacgc catgaatttt aaacggcgtg gcgaggcagg cagaaacgcc
    53161 ggatgccatc gctttggtga ccacatacgg gccaaccgct ttcaggccgc gcgggccgcg
    53221 cattgcgtca gcgccgaata cctggaaacg cggggaaccg ccgccagaac ctgcaatcag
    53281 gccaacgcgc gggttattct ggtaagcttc cggagaaagg cccgcatctg cgattgcctg
    53341 ctccatagaa aggaatgcat aaatggatgc gtcgctcata aagcgcacaa ctttgcggtc
    53401 aatgaggcca gtggtatcca gttttacgtt gccccagacg tggctacgca tgccggaatc
    53461 cttcagctcc tgagagaaag tgatccctga acgtccttca cgcagagatg ccaggacttc
    53521 ctgctggtta ttaccgatgc tggaaacaat gcccaggcca gtaatcactg cacgtttcat
    53581 tcaatacctc tgtaagtcgc acatagagta agtttcgaat gcacaatagc gtacacttgt
    53641 acgccgaaca agtccgatca gccatttaat agagaaattt gcgcagcctt acacacatcg
    53701 ctaagatcga gccaccgcct gtaagacgag taacttacgt gaaacactac tccatacaac
    53761 ctgccaacct cgaatttaat gctgagggta cacctgtttc ccgagatttt gacgatgtct
    53821 atttttccaa cgataacggg ctggaagaga cgcgttatgt ttttctggga ggcaaccaat
    53881 tagaggtacg ctttcctgag catccacatc ctctgtttgt ggtagcagag agcggcttcg
    53941 gcaccggatt aaacttcctg acgctatggc aggcatttga tcagtttcgc gaagcgcatc
    54001 cgcaagcgca attacaacgc ttacatttca ttagttttga gaaatttccc ctcacccgtg
    54061 cggatttagc cttagcgcat caacactggc cggaactggc tccgtgggca gaacaacttc
    54121 aggcgcagtg gccaatgccc ttgcccggtt gccatcgttt attgctcgat gaaggccgcg
    54181 tgacgctgga tttatggttt ggcgatatta acgaactgac cagccaactg gacgattcgc
    54241 taaatcaaaa agtagatgcc tggtttctgg acggctttgc gccagcgaaa aacccggata
    54301 tgtggacgca aaatctgttt aacgccatgg caaagttggc gcgtccgggc ggcacgctgg
    54361 cgacatttac gtctgccggt tttgtccgcc gcggtttgca ggacgccgga ttcacgatgc
    54421 aaaaacgtaa gggctttggg cgcaaacggg aaatgctttg cggggtgatg gaacagacat
    54481 taccgctccc ctgctccgcg ccgtggttta accgcacggg cagcagcaaa cgggaagcgg
    54541 cgattatcgg cggtggtatt gccagcgcgt tgttgtcgct ggcgctatta cggcgcggct
    54601 ggcaggtaac gctttattgc gcggatgagg cccccgcact gggtgcttcc ggcaatcgcc
    54661 agggggcgct gtatccgtta ttaagcaaac acgatgaggc gctaaaccgc tttttctcta
    54721 atgcgtttac ttttgctcgt cggttttacg accaattacc cgttaaattt gatcatgact
    54781 ggtgcggcgt cacgcagtta ggctgggatg agaaaagcca gcataaaatc gcacagatgt
    54841 tgtcaatgga tttacccgca gaactggctg tagccgttga ggcaaatgcg gttgaacaaa
    54901 ttacgggcgt tgcgacaaat tgcagcggca ttacttatcc gcaaggtggt tggctgtgcc
    54961 cagcagaact gacccgtaat gtgctggaac tggcgcaaca gcagggtttg cagatttatt
    55021 atcaatatca gttacagaat ttatcccgta aggatgactg ttggttgttg aattttgcag
    55081 gagatcagca agcaacacac agcgtagtgg tactggcgaa cgggcatcaa atcagccgat
    55141 tcagccaaac gtcgactctc ccggtgtatt cggttgccgg gcaggtcagc catattccga
    55201 caacgccgga actggcagag ctgaagcagg tgctgtgcta tgacggttat ctcacgccac
    55261 aaaatccggc gaatcaacat cattgtattg gtgccagtta tcatcgcggc agcgaagata
    55321 cggcgtacag tgaggacgat cagcagcaga atcgccagcg gttgattgat tgtttcccgc
    55381 aggcacagtg ggcaaaagag gttgatgtca gtgataaaga ggcgcgctgc ggtgtgcgtt
    55441 gtgccacccg cgatcatctg ccaatggtag gcaatgttcc cgattatgag gcaacactcg
    55501 tggaatatgc gtcgttggcg gagcagaaag atgaggcggt aagcgcgccg gtttttgacg
    55561 atctctttat gtttgcggct ttaggttctc gcggtttgtg ttctgccccg ctgtgtgccg
    55621 agattctggc ggcgcagatg agcgacgaac cgattccgat ggatgccagt acgctggcgg
    55681 cgttaaaccc gaatcggtta tgggtgcgga aattgttgaa gggtaaagcg gttaaggcgg
    55741 ggtaatctgc tctcgcagtg tttgccggat gcggcgtgaa cgccttatcc gacctacgtg
    55801 tgac
//
LOCUS       DAZKIV010000012        53899 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZKIV010000012 DAZKIV010000000
VERSION     DAZKIV010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53899
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.012"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            174..2417
                     /gene="pdeF"
                     /locus_tag="ACHOT6_000789"
     CDS             174..2417
                     /gene="pdeF"
                     /locus_tag="ACHOT6_000789"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFP9946054.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2456..3997)
                     /gene="ppx"
                     /locus_tag="ACHOT6_000790"
     CDS             complement(2456..3997)
                     /gene="ppx"
                     /locus_tag="ACHOT6_000790"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFP9946055.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHCNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(4002..6068)
                     /gene="ppk1"
                     /locus_tag="ACHOT6_000791"
     CDS             complement(4002..6068)
                     /gene="ppk1"
                     /locus_tag="ACHOT6_000791"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFP9946056.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFNQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6239..6877)
                     /gene="purN"
                     /locus_tag="ACHOT6_000792"
     CDS             complement(6239..6877)
                     /gene="purN"
                     /locus_tag="ACHOT6_000792"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFP9946057.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6877..7914)
                     /gene="purM"
                     /locus_tag="ACHOT6_000793"
     CDS             complement(6877..7914)
                     /gene="purM"
                     /locus_tag="ACHOT6_000793"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFP9946058.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTQLDGKPL
                     ADYLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     DSDSEQRVVIE"
     gene            8239..8865
                     /gene="upp"
                     /locus_tag="ACHOT6_000794"
     CDS             8239..8865
                     /gene="upp"
                     /locus_tag="ACHOT6_000794"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFP9946059.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8951..10240
                     /gene="uraA"
                     /locus_tag="ACHOT6_000795"
     CDS             8951..10240
                     /gene="uraA"
                     /locus_tag="ACHOT6_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFP9946060.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10335..11036
                     /gene="hda"
                     /locus_tag="ACHOT6_000796"
     CDS             10335..11036
                     /gene="hda"
                     /locus_tag="ACHOT6_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFP9946061.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(11174..11533)
                     /gene="arsC"
                     /locus_tag="ACHOT6_000797"
     CDS             complement(11174..11533)
                     /gene="arsC"
                     /locus_tag="ACHOT6_000797"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFP9946062.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11554..13017)
                     /gene="bepA"
                     /locus_tag="ACHOT6_000798"
     CDS             complement(11554..13017)
                     /gene="bepA"
                     /locus_tag="ACHOT6_000798"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFP9946063.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13230..14291
                     /gene="yfgO"
                     /locus_tag="ACHOT6_000799"
     CDS             13230..14291
                     /gene="yfgO"
                     /locus_tag="ACHOT6_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9946064.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14329..15177)
                     /gene="focB"
                     /locus_tag="ACHOT6_000800"
     CDS             complement(14329..15177)
                     /gene="focB"
                     /locus_tag="ACHOT6_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFP9946065.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSICYRAI
                     YLRQES"
     gene            complement(15199..17211)
                     /gene="hyfR"
                     /locus_tag="ACHOT6_000801"
     CDS             complement(15199..17211)
                     /gene="hyfR"
                     /locus_tag="ACHOT6_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFP9946066.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNVYYRYHDDGSDVTATAEYQGEMIDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17241..17654)
                     /gene="hyfJ"
                     /locus_tag="ACHOT6_000802"
     CDS             complement(17241..17654)
                     /gene="hyfJ"
                     /locus_tag="ACHOT6_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFP9946067.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17647..18404)
                     /gene="hyfI"
                     /locus_tag="ACHOT6_000803"
                     /pseudo
     CDS             complement(17647..18404)
                     /gene="hyfI"
                     /locus_tag="ACHOT6_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(18401..18946)
                     /gene="hyfH"
                     /locus_tag="ACHOT6_000804"
     CDS             complement(18401..18946)
                     /gene="hyfH"
                     /locus_tag="ACHOT6_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HFP9946068.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18956..20671)
                     /gene="hyfG"
                     /locus_tag="ACHOT6_000805"
     CDS             complement(18956..20671)
                     /gene="hyfG"
                     /locus_tag="ACHOT6_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFP9946069.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVIHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     CPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVGGEQIVDADYRLLYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVTVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20661..22241)
                     /gene="hyfF"
                     /locus_tag="ACHOT6_000806"
     CDS             complement(20661..22241)
                     /gene="hyfF"
                     /locus_tag="ACHOT6_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFP9946070.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLEPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(22246..22896)
                     /gene="hyfE"
                     /locus_tag="ACHOT6_000807"
     CDS             complement(22246..22896)
                     /gene="hyfE"
                     /locus_tag="ACHOT6_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFP9946071.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22908..24345)
                     /gene="hyfD"
                     /locus_tag="ACHOT6_000808"
                     /pseudo
     CDS             complement(22908..24345)
                     /gene="hyfD"
                     /locus_tag="ACHOT6_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416979.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
     gene            complement(24362..25309)
                     /gene="hyfC"
                     /locus_tag="ACHOT6_000809"
     CDS             complement(24362..25309)
                     /gene="hyfC"
                     /locus_tag="ACHOT6_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFP9946072.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25320..27337)
                     /gene="hyfB"
                     /locus_tag="ACHOT6_000810"
                     /pseudo
     CDS             complement(25320..27337)
                     /gene="hyfB"
                     /locus_tag="ACHOT6_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27337..27954)
                     /gene="hyfA"
                     /locus_tag="ACHOT6_000811"
     CDS             complement(27337..27954)
                     /gene="hyfA"
                     /locus_tag="ACHOT6_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFP9946073.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RLVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(28207..28677)
                     /gene="bcp"
                     /locus_tag="ACHOT6_000812"
     CDS             complement(28207..28677)
                     /gene="bcp"
                     /locus_tag="ACHOT6_000812"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFP9946074.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28677..29249)
                     /gene="gcvR"
                     /locus_tag="ACHOT6_000813"
     CDS             complement(28677..29249)
                     /gene="gcvR"
                     /locus_tag="ACHOT6_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFP9946075.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29395..30273
                     /gene="dapA"
                     /locus_tag="ACHOT6_000814"
     CDS             29395..30273
                     /gene="dapA"
                     /locus_tag="ACHOT6_000814"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFP9946076.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30290..31324
                     /gene="bamC"
                     /locus_tag="ACHOT6_000815"
     CDS             30290..31324
                     /gene="bamC"
                     /locus_tag="ACHOT6_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFP9946077.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            31537..32250
                     /gene="purC"
                     /locus_tag="ACHOT6_000816"
     CDS             31537..32250
                     /gene="purC"
                     /locus_tag="ACHOT6_000816"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFP9946078.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32404..32631
                     /gene="vapB"
                     /locus_tag="ACHOT6_000817"
     CDS             32404..32631
                     /gene="vapB"
                     /locus_tag="ACHOT6_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFP9946079.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32631..33029
                     /locus_tag="ACHOT6_000818"
     CDS             32631..33029
                     /locus_tag="ACHOT6_000818"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFP9946080.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            33176..34039
                     /gene="ypfJ"
                     /locus_tag="ACHOT6_000819"
     CDS             33176..34039
                     /gene="ypfJ"
                     /locus_tag="ACHOT6_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFP9946081.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            34054..36069
                     /gene="tmcA"
                     /locus_tag="ACHOT6_000820"
     CDS             34054..36069
                     /gene="tmcA"
                     /locus_tag="ACHOT6_000820"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFP9946082.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQSIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREARSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            36143..36841
                     /gene="ypfH"
                     /locus_tag="ACHOT6_000821"
     CDS             36143..36841
                     /gene="ypfH"
                     /locus_tag="ACHOT6_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9946083.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMVLESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36951..37151)
                     /gene="ypfN"
                     /locus_tag="ACHOT6_000822"
     CDS             complement(36951..37151)
                     /gene="ypfN"
                     /locus_tag="ACHOT6_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFP9946084.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(37179..38306)
                     /gene="dapE"
                     /locus_tag="ACHOT6_000823"
     CDS             complement(37179..38306)
                     /gene="dapE"
                     /locus_tag="ACHOT6_000823"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFP9946085.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38310..38666)
                     /gene="yffB"
                     /locus_tag="ACHOT6_000824"
     CDS             complement(38310..38666)
                     /gene="yffB"
                     /locus_tag="ACHOT6_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFP9946086.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            39047..39106
                     /gene="ypfM"
                     /locus_tag="ACHOT6_000825"
     CDS             39047..39106
                     /gene="ypfM"
                     /locus_tag="ACHOT6_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFP9946087.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(39205..42318)
                     /gene="acrD"
                     /locus_tag="ACHOT6_000826"
     CDS             complement(39205..42318)
                     /gene="acrD"
                     /locus_tag="ACHOT6_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFP9946088.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(42482..44182)
                     /gene="narQ"
                     /locus_tag="ACHOT6_000827"
     CDS             complement(42482..44182)
                     /gene="narQ"
                     /locus_tag="ACHOT6_000827"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFP9946089.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            44388..46367
                     /gene="aegA"
                     /locus_tag="ACHOT6_000828"
     CDS             44388..46367
                     /gene="aegA"
                     /locus_tag="ACHOT6_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFP9946090.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAAFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            46435..47010
                     /gene="nudK"
                     /locus_tag="ACHOT6_000829"
     CDS             46435..47010
                     /gene="nudK"
                     /locus_tag="ACHOT6_000829"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFP9946091.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            47136..48179
                     /gene="ypfG"
                     /locus_tag="ACHOT6_000830"
     CDS             47136..48179
                     /gene="ypfG"
                     /locus_tag="ACHOT6_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFP9946092.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(48275..50278)
                     /gene="tkt"
                     /locus_tag="ACHOT6_000831"
     CDS             complement(48275..50278)
                     /gene="tkt"
                     /locus_tag="ACHOT6_000831"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9946093.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50298..51248)
                     /gene="tal"
                     /locus_tag="ACHOT6_000832"
     CDS             complement(50298..51248)
                     /gene="tal"
                     /locus_tag="ACHOT6_000832"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9946094.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            51537..53816
                     /gene="maeB"
                     /locus_tag="ACHOT6_000833"
     CDS             51537..53816
                     /gene="maeB"
                     /locus_tag="ACHOT6_000833"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFP9946095.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
BASE COUNT        13101 a        13909 c        14181 g        12708 t
ORIGIN      
        1 acgtaataat tcattaatca gggttatttt gtttgatata tatcaattgg aactttcata
       61 tgacacttag aatcaatgct ctctatagtg atgaataatt atcattcgaa gtcaggtggg
      121 atgcctgtct gaatacacct ccttcaggat gtgggggatt ctgctgagca tctatgaaac
      181 tgaatgcaac ttatataaaa atacgtgata aatggtgggg gcttccgctg ttcttgcctt
      241 ctttaatctt gcccattttc gcccacatta atactttcgc gcatatttct tccggtgagg
      301 tttttctctt ttatctgcct ctggcactga tgatcagcat gatgatgttt ttcagctggg
      361 cggcattgcc agggatcgcc ttagggattt ttgtccgcaa atatgcagag ctgggttttt
      421 acgaaacgct atcattaacg gctaatttta ttatcattat cattctctgt tggggcggtt
      481 acagggtctt tactccccgg cgtaacaacg tttcacatgg tgatacccgt ttaatttccc
      541 agcgtatatt ctggcagatt gtgtttcctg caacgctgtt tctgatactt ttccagtttg
      601 ctgcatttgt aggattactg gcgagcagag aaaatctggt cggtgtcatg ccttttaacc
      661 tcgggacttt aatcaattat caggccttac tggtgggtaa tctgatcggt gtcccgctgt
      721 gctacttcat cattcgggta gtgcgaaatc cattttattt acgtagctat tattcgcaat
      781 taaaacagca ggttgatgcc aaagtcacca aaaaagagtt cgcgctctgg ctactggcat
      841 taggtgcttt attgttgctg ttatgcatgc cgttaaatga aaaaagcaca atttttagca
      901 ccaattatac cttgtcatta ttgctgcccc tgatgatgtg gggagcgatg cgctatggtt
      961 ataagctgat ttctctgctc tgggcggtcg tgttgatgat cagcatccac agctatcaaa
     1021 attacattcc catttatcct ggctatacca cgcagctgac cataacctcc tccagttatc
     1081 tggtattctc ttttattgtc aattatatgg ctgtactggc aacccgtcag agagcggtag
     1141 tcagacgcat tcagcggctt gcgtatgtgg acccggtggt tcatctgcca aatgttcgcg
     1201 ccctgaatcg cgcgttgcgt gatgccccct ggtctgcgct ttgttattta cgcatccctg
     1261 gcatggaaat gctggttaag aactatggca tcatgctgcg gattcaatac aagcaaaaac
     1321 tttctcactg gctgtcaccc ttgctggaac cgggtgaaga tgtttatcag ctttcgggta
     1381 acgatctcgc gctgcgactg aatacagaat cgcaccagga gcgcattacc gcactggata
     1441 gccatctcaa gcaatttcgt ttcttttggg atggcatgcc gatgcaaccg cagattggcg
     1501 tcagttactg ctatgtgcgc tcgccagtga atcatatcta cctgctgctg ggagagctaa
     1561 atacggtcgc cgaactttcc atcgtgacca acgccccgga aaatatgcag cgtcgcgggg
     1621 caatgtattt gcaacgcgaa ttgaaagata aagtcgcgat gatgaatcga ctacagcagg
     1681 cgctggaaca caaccatttt ttcctgatgg cccagccgat taccggtatg cgtggtgatg
     1741 tttaccatga aattcttctg cgcatgaaag gtgagaatga tgaactgatc agccccgata
     1801 gcttcttgcc ggtcgcgcac gaatttggtt tatcgtcgag tatcgacatg tgggtcattg
     1861 agcatacgct gcaatttatg gctgaaaaca gagcgaagat gcccgctcac cgttttgcta
     1921 ttaatctgtc tccaacctcg gtatgtcagg ctcgttttcc ttttgaagtc agtcagttgc
     1981 tggctaaata tcagattgaa gcgtggcaac ttatttttga agtcaccgaa agtaatgctc
     2041 tgaccaatgt taagcaggcg caaatcacct tgcagcatct tcaggaatta ggctgccaga
     2101 ttgcgattga tgatttcggc accggctacg ccagctatgc acggcttaaa aatgtgaatg
     2161 ccgatctgct taaaattgac ggcagtttta tccgcaatat tgtgtcaaat agtctggatt
     2221 atcagatagt ggcatcgatt tgccacctgg cgcgaatgaa gaaaatgcgg gtagtggcag
     2281 agtacgttga aaacgaagag atccgcgagg cggtgctctc tttggggatc gattatatgc
     2341 agggttatct tattggtaag ccgcagccgt taattgatac gttgaatgaa atcgaaccca
     2401 ttcgcgaaag tgcctgaata atgcgggccg acatttctcg tcggcccgca aagcattaag
     2461 cggcgatttc aggtgtactt tcttcttcaa ttttcaaccg ccagccagcc acgccttccc
     2521 agtattcttg ctccttttcc agatcaagca gtaccaacgc attctgacta aaccagtcat
     2581 gcgggaaacg cagtgtccag tgactgtcat cagtaatcag tgtcaatgtt ggcggtgtgg
     2641 tggttgcctg acgttgattg ttgagtaata cgccaaggcg caatagctga atcagtggca
     2701 ggaactgttt cttcttaaac aaggtaaagc gcggcagatc gtcgagttta atcgctttac
     2761 ggtgatagcg caccagtgtc gccatcatca gctgctgttc ctgattaaaa cccggcaagt
     2821 cactgttttg cagaatataa gcggagtggc gatgcaaacc gctgtggttg atattcaacc
     2881 cgacctcatg cagcatggcg gcccatcgca gtagcgcctc cagttgcgga tgcgccagct
     2941 tcggttgctg ttcccgccac tgttcgtaca tttgcatagt ggtatccagc acccgtcggg
     3001 cctgctcgct gtcgatgtga tactggttgg cgaggctgct ggcggtgcga ctacgcacat
     3061 cctgatgacg gaaacgtcct tccatttcat acagtacgcc ttcgcgtaac gccccgtcag
     3121 aaaggcgcag ttcacggatg gctaaagcat caaacacacc gcataaaatc gccagtccag
     3181 gaacgaagac tgttttccgc tcttcggaaa gacccggtaa actcagcgat gcgaaattac
     3241 agtgacgtaa aacttctttt accagttttt ccagacgttc cggggtaatt atcccgtctt
     3301 tctcgcccat ttccattaac acttcatggg cggcttttat ggtaccggaa gcgcccattg
     3361 ccacgttcca gccctgaata cggaattgcc aggttaaagt ttccagtttt tgtgctgccg
     3421 ccatacgagc gcgctgaaaa ttctctttat tgatgacccc gccagggaag taaagctggg
     3481 caaagctgac acaacccatc cggcggcttt caacgagaat cggttcgaaa ttttcaccaa
     3541 tcaccagttc cgtagatccg ccgccaatat caataaccag tttgcgacct ttctccggtt
     3601 gggtatgttc cacgcccata aaaatcagac gggcttcttc attaccggaa ataatttcaa
     3661 tcgggtaggg aatgaccttt tccgcgcgtt tcagaaagtc agtggcgttc agcgcctgac
     3721 gcagcgtatg ggtgccgact atacagacgc tggcaggaga aaacccttgt agccgttcgg
     3781 caaacagcga cagacagttc aaaccgcgcg tcattgcctc ttcactcaac atattatctg
     3841 gccccaggcc gtccgccaga tgcactcgct gtttcaggcg gccaataatc tgcatggcac
     3901 catctaccac acgggctatg accatgtgaa aactgtttga accaagatcg accgcagcaa
     3961 actcctgcgg acgaggggat ttatcgtgta ttggcatagg gttattcagg ttgttcgagt
     4021 gatttgatgt agtcgtaaat cgccaactgc gcccgtactt tgcggcgatt gccgcgggga
     4081 acgtagcgat tactgagttc tttatcgata taacgtgctt tgaccgtatc gctgaacaat
     4141 atgtcgatga tgtccagcac ccgctgcttc aggcgcggat cgagcagcgg tgtcgccact
     4201 tcaatacgat aatcaatatt gcgcgtcatc cagtcggcgg aagaaaggta gaccttttta
     4261 tcgccgccat tttcaaaaat ataaacccgg tcatgttcaa ggtaacggtc aacaatactg
     4321 atggcacgaa tgttgtcgct aatgccttcc agattgggga tcagcgaaca cattccgcga
     4381 accagcagat taaccggtac gccggagctg gaggccgcat acagacgatc aaccaggcct
     4441 ttatcgacaa ggttatttag cttcagggtg ataccactgg gcagcccttg ctgcgcgttg
     4501 gcgatctcgc ggtccaccat ttcatacaac agacggcggg agttttgcgg cgacaccatt
     4561 aaataatcaa atgtcaccgg acggtatggg ttttcaataa agttgaacac ccgccgtact
     4621 tcgttagtga tgcgcgcatc tgcggtcagc aacgaatagt cagtataaag acgcgcggtt
     4681 ttttcgttaa agttcccggt cccgatgtgt gcataacgca ccacttcacc gttttcttta
     4741 cgtgaaatca ggaacagttt ggcgtgaatt ttcagccccg gcgcagagaa gataacgtgc
     4801 acgcctgctt cggtcaggcg cttcgcccag tgaatgttgg cttcttcgtc gaaacgcgcc
     4861 tgtaactcaa ccaccacggt gactttctta ccgttatgtg cggcgtggat catcgagtcg
     4921 atgatgcgtg aatctttcgc cacgcggtaa atgttaattt taatcgccag tacgctcggg
     4981 tcgaacgaag cctgacgcag cagttccagc acatgctcaa aggtgtgata aggataatag
     5041 agcaacacat cgcgttcgcg aatggcatca aaaccattgc ggaactgggc tttatcaaac
     5101 caaatgtggc gtaaacgcgg cagtggtttg ttcaccagat tggctttgcc gacattgggg
     5161 aaattaataa agtctttaaa attatgataa cgaccgccgg ggacgatgga gtcgtagcgg
     5221 gaaatagtca gtttttcgcg taacacttca accagcgcat tgggcatatc gcgctgataa
     5281 acaaaacgca ccggctcagc agttaaacgc tgcttgagac tggaagacat caactccatc
     5341 aggctggctt ccatctcatg cactaaatcg tattcggcat cgcgggtcat cttcattgaa
     5401 taggcattca gcgcgtcata atcaaagaag cctttgaaaa tatcatcaag gcagtaacgc
     5461 agaatgttat ccagaagaat catcggcttg cgtcgacgcg gtgcttctgg cggtaaattc
     5521 acaaagcgcg gcactttatc tgatgggatc tccagcagcg cgtaacggat ggtatcgcca
     5581 cggataattt ccaccgccag ataggtgtaa tcatctttca ggaactgcac taagtcagtg
     5641 tcaggattga ttaaaatcgg cgtaatgtgc tgacgcagat actgattaaa ataatgacga
     5701 agccagtttt gttgattgac ggagagctgg cgttcattaa tcaggaagat ctggttacgc
     5761 gccatctcca gcagcagctc gttgtagagg ccgtcgaatt cctgatcggc tttcagcacc
     5821 cgggactgaa ttttgcccag taaatggcgg gaatgagagt tggagccttg ttcttcgcta
     5881 ataatgatgc gtcgcttcag ttcagcgaag cggactttat agaactcatc aaggttattg
     5941 gaatagatcc ccaggaaacg catcctttca atcagcgggt tagatttgtc cgccgcttcc
     6001 tgaagcacgc gttcattgaa cgataaccaa ctgagctctt tttcgatgta tagcttttcc
     6061 tgacccatta ccacttttac tccgttttat tcacgggaca ctgcctggat attatggcga
     6121 gcaatattgg cagatgtcca actatgccag aaaaggggga aaaaaactgc tggagcttgc
     6181 gatttacagg tgaattgaaa aacgccagcg gcagagctgg cgctttaact acggggcatt
     6241 actcgtcggc agcgtagccc tgcggcggca gacgttgacc atccagccac gcggcgtttt
     6301 cgtgcatttt cagacgacca tcggcaaacc agctaatcac cagtggataa atagcgtgtt
     6361 cctgggtttg cacgcgggcg gtgatgtcat cttccgtatc accagcaaat accgggactt
     6421 tcgcctgtaa aataaccggg ccaccgtcca gttcatcggt gacgaaatgc accgatgtac
     6481 cgtgctcttc atcaccattt tccagcgctt gacgatgggt gtgtaatccg ggatatttcg
     6541 gcagcagaga agggtgaatg ttcagcaaac gcccggcata gtgggagaca aacgccgggc
     6601 tgagaatgcg cataaaacca gccagcacga ccacatcggg tgcgtacatg tcgatttcat
     6661 gaatcaactc ccggtcatag gcttcacgac tgtcaaacgc gctggcgatg agcgtatgcg
     6721 ttgcaatacc cgcctggcgg gcgcgttcaa ggccgaacgc gtcggcctta ttgctgaaaa
     6781 ctgcccgtac ggtgccttta attttgttgg ttttacaggc gtcaataatt gcctgtaaat
     6841 tacttccgtt accggaaata agcaccacaa tattcattat tcgataacca cgcgttgttc
     6901 ggaatcagag tctttgatga taccgatttt ccacgcgttt tcaccgttgg cattgagcag
     6961 ggcgagggct ttgtccactt ccggagcagg cagggcaata atcatcccga cgccgcagtt
     7021 gaaggtgcga tacatttcat ggcgctcaac gttacctgcc gtttgcagcc agttgaacac
     7081 ttccggccac tgccaggaag attcatcaat cactgcctga gtattatctg gcaatacgcg
     7141 tggaatgttt tcccagaagc cgccgccggt caggtgcgca atggcatgca catcgacctt
     7201 ttcaatcaac tccagcactg acttcacgta aatgcgggtc ggtgccagca gataatcggc
     7261 taatggctta ccatcaagtt gggtggtttg cggatcacaa ccgctgactt caagaatttt
     7321 gcgcaccagc gaatagccgt tcgagtgcgg accgctggaa ccgagtgcaa tcagcacatc
     7381 gccgtcgctg actttagagc cgtcgatgat ttctgatttt tctaccacgc caacgcagaa
     7441 acccgcgaca tcgtaatcct caccgtgata catccccggc atttctgccg tttcgccacc
     7501 caccagtgaa cagcccgatt gcagacatcc ttccgcgata ccgctgatca ccgctgaagc
     7561 ggtatcaaca tccagttttc cggttgcgta atagtcgagg aaaaacagcg gctctgcacc
     7621 ttgcaccacc aggtcattaa cgcacatggc gaccagatca ataccaatgg tgtcgtgacg
     7681 ttttaagtcc attgccagac gcagcttggt acctacgccg tcagtgccgg aaaccagcac
     7741 gggttcacga tatttttgcg gcaatgcaca cagcgcaccg aagccgccca gaccgcccat
     7801 cacttccgga cgacgcgttt tcttcactac gcctttgatt cttccaacca gagcattacc
     7861 cgcgtcaata tcaacaccgg catctttgta gctaagagag gttttatcgg tcactgcttg
     7921 ggtccccacg cgttacttgc ggtagaaaaa taaaattcgg cgcaattcta acagggaaag
     7981 caaacgtttg cgagactgct ttacacaacc tttttgcacg tcttttgccc aggcgcgcgg
     8041 cgaaagaaga cttgtaccag ggtaaaggtt agttttcgga tggaataatc ttctttcata
     8101 accatttgaa tataaaataa ctttatctca agccgttatc atattgacta aagtcaacga
     8161 aaagaatatt gccgccttga agaaaggagg tataatccgt cgattttttt tgtggctgcc
     8221 cctcaaagga gaaagagtat gaagatcgtg gaagtcaaac acccactcgt caaacacaag
     8281 ctgggactga tgcgtgagca agatatcagc accaagcgct ttcgcgaact cgcttccgaa
     8341 gtgggtagcc tgctgactta cgaagcgacc gccgacctcg aaacggaaaa agtaactatc
     8401 gaaggctgga acggcccggt agaaatcgac cagatcaaag gtaagaaaat taccgttgtg
     8461 ccaattctgc gtgccggtct ggggatgatg gacggtgtgc tggaaaatgt tccgagcgcg
     8521 cgcatcagcg ttgtcggtat gtaccgtaat gaagaaacgc tggagccggt accgtacttc
     8581 cagaaactgg tttctaacat cgatgagcgt atggcgctga tcgttgaccc aatgctggca
     8641 accggtggtt ccgttatcgc gaccatcgac ctgctgaaaa aagcgggctg cagcagcatc
     8701 aaagttctgg tgctggtagc tgcgccagaa ggtatcgctg cgctggaaaa agcgcacccg
     8761 gacgtcgaac tgtataccgc atcgattgat cagggactga acgagcacgg atacattatt
     8821 ccgggcctcg gcgatgccgg tgacaaaatc tttggtacga aataaagaat aaaaataatt
     8881 aaagccgact ttaagagtcg gctttttttt gagtaaagcg cctataacac ataatacaga
     8941 ggataatact atgacgcgcc gtgctatcgg ggtgagtgaa agaccgccac ttttacagac
     9001 aatcccgctt agtttgcaac atttgttcgc catgtttggt gcaaccgtcc tggtgcccgt
     9061 cttatttcat attaacccgg cgactgtact gttatttaac ggtattggaa cgctgctgta
     9121 tctcttcatc tgtaaaggga aaattccggc ttatcttggt tccagctttg cctttatttc
     9181 accggtattg ttactgttgc cgttagggta tgaagtcgcg ctgggcggct ttattatgtg
     9241 cggcgtgctg ttctgcctgg tttcttttat cgtgaagaaa gcggggaccg gctggctgga
     9301 cgtgctgttt ccacctgcgg caatgggcgc aatcgttgcc gtcatcggtc tggagctggc
     9361 gggcgtagct gccggtatgg cgggtttact cccggctgaa gggcaaacgc cagactccaa
     9421 aaccatcatc atctctatta ccaccctggc ggtcacggtt ttaggttccg tgctgtttcg
     9481 tggtttcctg gcaattatcc cgattttaat tggcgtgctg gtgggctacg cgctctcttt
     9541 cgcaatggga attgtcgata ccacgccgat tattaatgct cactggtttg cgctgccaac
     9601 cctctatacg ccgcgcttcg agtggtttgc cattctgact attctgccag cggcgttagt
     9661 ggttattgcc gaacacgtag ggcacctggt agtaacggct aatatcgtca aaaaagatct
     9721 gctgcgcgat ccaggtctgc accgttcgat gtttgctaac ggcctgtcga ccgtgatctc
     9781 cggcttcttt ggctctacgc caaataccac ttacggagaa aacattggcg tgatggcgat
     9841 cacccgtgtt tacagtacct gggttatcgg tggggcggcg attttcgcta tcctgctttc
     9901 ctgcgtcggt aaactggctg ccgctatcca gatgatccca ttgccggtga tgggcggcgt
     9961 ttcgctgctg ctttatggtg tcatcggtgc ttccggtatt cgtgttttga tcgaatcgaa
    10021 agtggattac aacaaagcac agaacctgat cctgacttcc gtgattttga tcatcggcgt
    10081 cagtggcgcg aaggtaaaca tcggcgcggc ggagttaaaa ggtatggcgc tggcgaccat
    10141 cgtcggtatc ggcttaagtt tgatcttcaa actgattagt gtgctgcgcc ctgaagaagt
    10201 ggtgctggac gcagaagacg cggatataac agacaaataa tgtaccgacc gggcagtgtt
    10261 gctgcccggt tcaaacatca tgggattctg tggtaaactt ctcgcgattt tgtgaaatcc
    10321 tggttgaggt atctctgaac acaccggcac agctctcttt gccactttat cttcctgacg
    10381 acgaaacctt tgcaagtttc tggccggggg ataactcctc tttactggct gcgctgcaaa
    10441 acgtgctgcg tcaggaacat agcggttaca tctatctctg ggcacgcgaa ggcgcggggc
    10501 gcagccatct gctgcacgcg gcttgcgcgg aattgtcgca gcgtggcgat gcggtgggct
    10561 atgtcccgct ggataaacgc acctggtttg ttccggaagt gctcgacggt atggagcatt
    10621 tgtcgctggt ctgtatcgac aacattgagt gtattgcagg cgatgagttg tgggagatgg
    10681 cgattttcga tctctacaat cgaattctgg aatcgggcaa aacacgattg ttgatcaccg
    10741 gcgatcgtcc accgcggcag ttgaatctgg gattaccgga tctcgcgtcg cgactcgact
    10801 gggggcagat ctacaaattg cagccacttt ctgatgaaga taagttgcag gcgctacagt
    10861 tacgcgcgcg tttgcgtggt tttgaactgc cggaagatgt ggggcgtttc ttgctgaagc
    10921 ggctcgacag agaaatgcgc acgctattta tgacgttgga tcagttggat cgtgcgtcga
    10981 ttaccgcgca acgtaagctg accattccgt ttgtgaaaga aattctgaag ttgtagataa
    11041 ggtgtttatt gtcggatgcg gcgcgaacgc cttatccgaa ctacgcgaag ttaatccata
    11101 gtattcgtag gccggataag acgtggcaag cgtcgcatcc ggcaatgtgc ttcaactagc
    11161 gatggagcac atcttaaccc acgatttcca gtacctgctc cggtggacga ccaatccgcg
    11221 ctttgccatt cgcgaccaca atcgggcgct ccatcagctt cggattatcc accattgcct
    11281 gaatcagcgc ctcttcgcta agcgaactgt cagccaggtt cagttcttta taaagatcct
    11341 ctttctggcg catcaattca cgggcgctat tcatccccag cattttcagc aaatcgcgca
    11401 gcgttgccgc atcggcgggt gtctcaaggt agagtaccac ttctggctcc acgccgtttt
    11461 ctttcagcag gttcagcgtt tcccggctct ttgaacagcg tgggttatgg tagattttta
    11521 cctgtttggt cattgttctt cctttaatgc gaattacatc ttggtataag gcttaaagcg
    11581 ttcctgcagc tggcgcaact ggtcgatgcg cgcatcgtaa cgcgcttgtt gcaggctgcc
    11641 taatttcacc tgcgaactgg cgctactcaa cagcgaaatg gcctgatcga gtcgtccggc
    11701 gagcgcataa ccttctgctc gcgcagccag ctcctgatcg cggttattta gcgcggcttc
    11761 cgcctgtgcc agtaaatccc agccgttgct gtcatcttta ttattaaagg tgtagcgatt
    11821 gagaatattg gccgcttctt gtggttgacc gccttgcaga taagcgtttg ccaggttgag
    11881 ctgcaacaca ggattggtgc gcaaatcacg ggcatttttc aggcgattga ttgcctcatt
    11941 ggctttgttt tgcccaagat cgatatcggt agccagatcg agataccatg cgttgccagg
    12001 ttctgccgcc agtaacggtt gcagcgtttt acgcgcctcg tcgtatttat tggcttccat
    12061 cgcctgtaaa gcacgaccat attgtgctgc tcgttgctga cgaacgtttc ctttcgccca
    12121 ttcatccagc aaatcactgg tgagctggtt acgtccggaa ttatacatcc ccagtgtgcg
    12181 cgctttcgcc agatagaaat cttccgacga ctgcaccacc atcgggcgca tctgattagc
    12241 acggttgcgg gcatctgcca gacgactttc cggcaacggg tgagtcaata aaatttccgg
    12301 cggacgcgag gagtaacgcg cctgatcgag caatttttcg aggaaggtcg gcatcgcctg
    12361 cggatcgaat cctgagcgtt gcagcacctg aataccaatg cggtccgctt cctgttcatt
    12421 ttgctgggtg aaactgatca tcccctgacg cgttcccgcc agtgtaccgg tcagcgccgc
    12481 catccccgcc tgcggactgg ccatcgccag taaaatagaa cctaacgcgc cgacccaggt
    12541 cagcggcgcg ctgcgctgct gatcttccat cgctcgcgcc aggtgacgtt gggtgacgtg
    12601 ggagatttcg tgcgccataa ctgaagccag ttgactttcg ttatcggaat aacggaacag
    12661 ggcagagtgc agcaccacgt tgccgccaaa gaaagcaaag gcgttaattt cgtcgttgtt
    12721 gatcagaaaa aaatgaaacg gtgtcttaac cgaattggca tgcgaaacca gacgcatccc
    12781 cagcgaatta atatattgcg ttaacagcgg gtcattaatt aacggcgcgc tgccgcgtag
    12841 ctggcggaca taatagtcgc ccatctgcat ttcctgacca atggaaagcg tgcttcctgc
    12901 ggaggttccc atatccggca aggtgtctgc gctgtcggca aaagccgggg ctacctgacc
    12961 aatggtcata gcagcaatga gggttgcaac caggtttttt ttcaactgcc tgaacataac
    13021 ctctatcctg tatttctgaa gatagtcatt tgaccgatac tgttaaatat cgttcccacc
    13081 gcgccgcctt tgagtgtaac ggcgaagatt agcccactca aatgactttt cagtttcctg
    13141 aatccgtcat aaaaagcgaa gtcttttttg tgacatttcg atacaattgc ggatcgcaat
    13201 cccgctcttg aggttcaggg aaggttttta tgctcgaaat gttgatgcaa tggtatcgcc
    13261 gccgttttag cgacccggaa gcgattgcct tgctggttat tttagttgcc ggatttggca
    13321 ttatcttttt ctttagtggc ctgcttgctc cgttgctggt ggctattgtg ctggcctatt
    13381 tgctggaatg gccaaccgtg cgcctgcaat ctattggctg ctcccgccgc tgggcgacgt
    13441 cgattgtatt ggtggttttt gtcggtatat tgctactgat ggcgttcgtg gtactgccta
    13501 tcgcctggca acagggcatc tacttaatcc gcgatatgcc ggggatgctc aataagcttt
    13561 ctgactttgc cgccacgttg ccgcgccgct atccggcgtt aatggacgcg ggcattattg
    13621 atgcaatggc cgaaaatatg cgcagtcgga tgctgaccat gggcgattcg gtggtgaaaa
    13681 tttccctcgc ctcgctggtc ggtttgctga ccattgccgt ctatctggtg ctggtgccat
    13741 tgatggtctt cttcctgctg aaagacaaag agcagatgct gaacgccgtt cgtcgggtgc
    13801 tgccgcgtaa ccgtgggctg gcaggacagg tgtggaagga gatgaatcaa caaatcacca
    13861 actatatccg cggcaaagtg ctggagatga tcgtggtagg gatcgccacc tggctggggt
    13921 tcttgctctt cgggctgaac tattcgctgc tgctggcggt gctggtcggc ttctcggttc
    13981 ttattccgta cattggcgca tttgtggtga ccattccggt ggttggcgtg gcgctattcc
    14041 agtttggcgc aggcacggaa ttctggagct gcttcgcggt gtatctgatt attcaggcgc
    14101 tggacggcaa cctgctagta ccggtgttgt tctccgaagc ggttaacctg catccgctgg
    14161 tgattatttt atcggtggtg atcttcggtg gtttgtgggg attctggggc gtattcttcg
    14221 ccattccatt ggcgacgctg atcaaagccg tgatccacgc ctggcccgat gggcaaatcg
    14281 cgcaagaata atcttcagcc cgacgaactg tttcgccggg ctgatttttc aggattcctg
    14341 acgtaaataa atagcccgat aacaaatact caccagcact gcaccgccga taatattacc
    14401 cagcatcacc gggagcagat tggcggtaat aaaatggctg accgtcagtg ccggaaaatt
    14461 gtctgcgcta ctgtgcgcca gttgccagaa ggaggtaggg gcgaaatggc gaatggcaat
    14521 ggcgaacgga atcacaaaca aattggcgat acagtgctca aagccactgg cgacaaacag
    14581 ggtgatgggc aaaatcatgg cgacgatttt gtcgcataac gaacgcccgc aataactcat
    14641 ccacagcgcc aggcaaacca tcagattgca cataatgccg aggctgacag attcagtaaa
    14701 tgtatgatgc attttgccct cggcgcagtg taaaaccgcg actccccaca tcgcgttttc
    14761 actcatcacc agcccggaaa accagattaa caaactgaaa caggcaatac ctgccagatt
    14821 accgcaggcc accagaagtg cgttaatcag ccaagttcgc caactaataa cgccccgact
    14881 ttttgccatt accgtcatta ccgacgaggt gaacaggttg gtgccgcaaa ccgccagcaa
    14941 aataaagcca agtgtaaagc aaaggccgcc caccagatgg gttaatgcct gtgaagacgg
    15001 ggcatcggcg ataactgaaa ggtaaaaagt aaatccgatc gccataaaca cgccagcgga
    15061 catcgccatt aaaaagaccg acactttact gcgggcaatt ttatgggcgg caatccgttc
    15121 agcgattgcc gcttttctgg cgctcaactg caagtcgaaa gagagtttgt ttctcatgca
    15181 tactcctgca aaagcagatt acaacacctc gcgaaccgag attcccagac gctgcattcg
    15241 tgacagcagc gtggtgcgct tcatccctaa tcgtgtcgcc gcgccacggg gcccggcaac
    15301 aatgccattg gtttctcgca atacctgaac aatgcgctga cgttcttctt cgtcattctc
    15361 tggcgtcgtc gggtgcagca actgcgccat tgaactgcga agcgctgaat cttcatttag
    15421 cgtcggcagt aaacggcttt gtcggacatt aagatgtaaa ttcagactgt taccgcgagt
    15481 cagcagtacc gctcgctcaa tcacgttttc cagctcgcgc acgttgcccg gccagtccca
    15541 cgacatcaac tggcgtagcg cctcggtcgg gatggcgtca attgaacgat tcatatggcg
    15601 cgccattttt tgcgtgaaat gctttgctaa aagagggata tcttccggac ggtcgcgtag
    15661 cggcggcaat tccagtggga agacattcag gcgataaaag agatcgctgc gaaactggcg
    15721 atcttcaacc atttgccaca aatcacggtt ggtggcggca atgactctga catttaccgg
    15781 gatcgttcta ctcccgccga gacgctcaat ctcccgttcc tgcaatacgc gcagcagttt
    15841 aggctgaagt tctaacggca gatcgccaat ttcatcgaga aacaacgtgc cgccatcggc
    15901 aatttcaaaa cgaccacgat gggtattaat cgcaccagta aacgcccctt tgtcatgacc
    15961 gaataactca ctttccagaa gactggcggg gatggcagcg cagttgattt tgaccagcgg
    16021 cttgtcgcgt cgcgggctaa gttgatggat cgctctggcg atcacctctt tgccggttcc
    16081 ggtttcaccg caaatcaaca ccgtactgtc gctcttcgcc acaatatcta cctgctggag
    16141 taggtcttcc attgcctggc tttgatagat aatgtcaccg acgcccagat tcgaaaggag
    16201 ctgctcgcta agctggtggt tttcttgctg caaactttcc tgcaaatcgg tcattctacg
    16261 ccaggcatcg gcattgccaa cggcaatagc gatgcgatcg gctatgtgtt gtagtagctg
    16321 gcagttttcc tcactaaaga gagtggaaga ggtatgcgcc agcaacaatg cacccggtgt
    16381 atggttgcca aaggtaagcg gtataaggag cgcagattcg cagccgttag atgcaagtaa
    16441 gagtaataac gggtcgcgtt gccagagaaa cagatcatct gcacggtgcg tcaaggtcgg
    16501 ttgtcgggtt tgtagcgttt gtgtcagcaa tacactgtcg ccaggcatat tgcgtggcag
    16561 acacgcacaa tgtgaggcag aaagatcgct gcaccacagg ctgaacttct cgttctttcg
    16621 atgatcgccc agtaccatac tgactgaagc cagaccgaaa aaatgatgga tctcacgagc
    16681 gacgtcagcg atcagatcgt cgagatcaag atgtgacagc actgcattag tgatatccac
    16741 cagtacccga tgatgatcgc gatctttctc cagcaaacgc agcgccacct gctgttcttc
    16801 atcattgagc catgattgca gaaccggggt gataatgcgt gccagccagt taaacagtgt
    16861 gcaatcctgg ggagtaaaac ggcagccgtg ggtgcgaata aagcgtagct ccgccattcc
    16921 actactgccg agaaggacgg cgtgacgcga ataatcgatc atctcgccct gatactctgc
    16981 tgttgccgtc acatcgctgc catcatcatg gtagcggtaa tagacgttgc ttttgagaac
    17041 atgcagttca ataagttgta ccgggctgaa cggcggcggc agcgggatga aggcgcgtaa
    17101 taaagacgcc ttaccgtgtt tctgcgctaa ccgctccgcg agcattccgt ttaccgcttc
    17161 aattgttatt ccttgcggcg gggcaaacat cgcctcgtct gacatagcca tcaacagtcc
    17221 ttattaatga gaaatccttc tcacgcaatt tttctggcca tcagatagat ggccggttcc
    17281 tgcacaattt catccaacag atgaatgaat tgaatgctac gcgcttgcca cacagacgtc
    17341 gcgtcatctg ccagtggcgc gaatgcccgg gtcaacaggg cggtgtggct ggcgtcaata
    17401 acaatctcac caaaagtcat cacccccagc atcttacgtc gggcttgctc ctcttcgacc
    17461 agtgcaagcc aatcttcgta ttccgtcagt gggcagcgga aggcgacatt cagacaatca
    17521 atcacgccaa cgtgatgacc gatagccagc gagtaataca ttacctgaga gctgtgttcc
    17581 ggcatctcgt cgctactggc gacaaacttt tttcgcagcg tccagaaaac aatttccccg
    17641 cactcttcag tcatgtaacc ccctgagtac gcgaaagagt tgctgaacga tactattgag
    17701 acgtggatcg tcggcatcgc gcaaccaggt gttaacccga tttcctgtag gatcgtcgct
    17761 taaatggcgc aggagccgat cgcaaatttc tcgcccctga cgatagcccg ccagccgccg
    17821 cgcttctcgc tcaatggcga tacgctgtga tggcgggatc tgtggccaca acggttgcat
    17881 agtcaccccg gtgggatcgc gataatccac agcgtgaatc ttctgttgca gcaaaccgag
    17941 cgccacggcg aaaccgtgaa tggtggcggc cggtgttggc gggcagccgg ggatccaaac
    18001 atcaatgggg acaatggtgt cgctgccgcc ccagacgctg tagagatcgt ggaaaatacc
    18061 gccgccgaca ccgcacgcgc cgtaggaaac acagatttat gatcgggggc agactcatac
    18121 gcccgaagtg caggcatacg catcgcccgg gtgactgcgc cagtaaataa caaaatatcg
    18181 gcgtgacgcg gtgatgaaac aaccttaatg ccaaaacgtt ctgcgtcgaa tacgggggtg
    18241 atggcagcaa aaatttcgat ttcacaggcg ttgcagccac cgcaatcgac gcgataaacg
    18301 taggctgagc gacggatatc ctgtagcaaa tgccgcttca tattttgcgt ttgctcgtcc
    18361 agcgtgatgg gctggctgac atattgcgta agcactggac tcatagctgc tccttagcca
    18421 ccagcggtac atctgtatcg tcgttgagca gcgtcgcgcg ttgtttgcac tccgggcaga
    18481 cgctcgcttg cgcccataac atttcgcggt tttgtggcgc attttgttgc tgtgccaaca
    18541 attcagcagc cagtgcgacg gttttttgcg gggcaaacgg gcgttcgcaa cggctgcaac
    18601 gttgtagatg gaacgtcgcg cgggtataga gatcggcttt attggtgacg gtcagttcaa
    18661 agttattggt aagctggatg gctctggtcg ggcacacttc ttcacaacgt ccgcagtaaa
    18721 tacaacgccc cagatagagc tgccaggtgc gcgaattttg ctggtcgtcg gtctggatag
    18781 tcagcgcatt tgccggacaa gcacaggcgc aggcaccgca ggcaatacat tggctgggca
    18841 tcaggtccgg tttcccgcga aagcctgggc tgacctccag tggcgcgaag ggatatttca
    18901 ccgtcgcggt tccggcgcgc ataatagttt tcagtaactt cagcatcttc tgtccttact
    18961 tcagcggcga acggttacga tcaatgccgt agcgttcgat ctctttatac ggcacggttt
    19021 ttgactggcg cttgcgcaca tctaccagcg tcacacggtc ggtacaggag tagcagggat
    19081 caaggctacc gataatcagc ggtgcgtcag aaacggtatt gccgcgcagc atgtaacgca
    19141 acaccggcca gttggcgtag gtggcggcac ggcagcgcca gcggaacaat ttttggttat
    19201 caccgagcat gctccagtgc acgtcttcac cgcgtggcgc ttcaacaaag cccagcgcga
    19261 atgcgtgagg tttatagcta aagccttcgg tcagcagtgg ggtatccggc atgttgtcga
    19321 gagcaaattc cagcattgcc agcgaatcaa acgtctcttt aacacggacc gtcacgcggg
    19381 agaaaacatc gccgccggta aaggtaaaca gtgtttttgg aatattgccg tagtcggcgt
    19441 aggggtgatc aaagcgcaaa tcacgggcaa aaccactgcc gcgaatcagc ggccctacag
    19501 ggctataatc acgggcgatt tgtcggtcga gaatgccaat gccctgagtg cgttgttcca
    19561 tattcggcgt agcaagcagc atctctacca gctccgacac gtcggcgcgc atctcgcgca
    19621 ccagtttcag cgtttgcaga cgttgctctt tgagaatatc gcggcgaaca ccaccaatca
    19681 gattcagacc gtaggtttta cgcgacccga tcagcaattc cgccatcgtc atcgactttt
    19741 cccgcacgcg gaaaaattgc ataaagccgg tatcaaaacc aacgaaatgg caggagaggc
    19801 caaggttaag caaatgactg tgtagccgtt cgacttccag cagaatcgag cgaatagtat
    19861 gtgctcgttg cggcacctca atccccagtg cattttcaac cgagttggta taggccacac
    19921 tgtgggcaaa accgcaaatc ccacacacgc ggtccgataa gaaggtcact tcgttgtagc
    19981 ccatccgcgt ttctgccagt ttctccatgc cgcgatggac atataacagg cggtaatcag
    20041 catcgacaat ttgctcgcca cccacaaaca agcggaagtg acccggttca tcggaggtga
    20101 tatgcagcgg gccgacaggg atcacccgcg catcgctgtt gccctcattg ataaacgcat
    20161 acgtttcgga atctgtcgtc ggttcagggc acagtcgata atccatcgca tctttgcgca
    20221 gcggatgcat atcttccggc cagtcatcgg gcaacaccag gcgacgctga tccggcaggc
    20281 caaccggaat cagcccgtac atatcgcgaa tttctcgctc gccccagacc gcggcaggga
    20341 cgcgcggtgt gactgacgga aactcccgac tgtcggcatc gaccagtgcc ttcaccacga
    20401 tccagcattt ttcggcccct tccattgaaa gggcataata aaccgcgtaa tgaccgttaa
    20461 gtgtccgctc gtcgttgcca aacaggactg gaagccagcc atcatgttga taataaagat
    20521 aatgtataac gtcaggcagc agattgattt tcaccgtaat ggtgacctgt tcaggcgttt
    20581 gtcgctcttc atccagcacc gcgccgggga actgcgtttt cagggcggcg agaatcgctt
    20641 caccacgatt tgacgatgaa ttaacgttca ctgtgtttct ccgaaacaga tgcggtgcct
    20701 gaaggcaaaa aatcatgcca ggtgctacgt tgtgcaggca ggtcgtgcgt ccctgagagg
    20761 actatagtgg aagcgcccgc caggatcctg atgacaggtt gtggaatatg cgttcccatc
    20821 gccagcatca tgaccagcag aatcaccatt ggcgaggtgg tcaaccagcc gagatcaccc
    20881 cggttaacgg cctgcggcgg tttcgccatt aacacccgcg ctgccatccg taccaggccc
    20941 gccagcacca gcgttaacag caataacagc aggacgataa tcagcaggtg attacgtgcc
    21001 agtccggcgg taatggtcat aaattcgcta agaaaaatgt tgaagggcgg catccctgcc
    21061 agcgccagcg caccgccgcc aaacagcacg gcggtaaatg gcatgatttt gagcatccca
    21121 cagacgacgt tgagatcgcg cgtgccgtac ttgagcagta cattgccgga accgcagaac
    21181 agcagcgttt ttgccagact gtggtttaag atgtgcagca gcgcggcaaa aattcccagc
    21241 ggcccgccaa tgcctagcgc caccgcgacc agccccatgt tctccacgct ggagtacgcc
    21301 agcagacgct taatgtcccg ctgtaccaga atgaaaaatg ccgccacggc aaccgacaac
    21361 atgccgaaga tgagcaacaa ccggttgggg aaatcgctgc cgatggcctg gcagataatg
    21421 atgtaatagc gaatcagcac caacagcgcg cagttcagca atacggcgga gagcagggcg
    21481 ctgaccggac tcggtgcttc actgtgagca tccggcagcc aggcgtgcat gggaaatagc
    21541 ccggttttgg taccaaagcc aattagcaca aacacaaagg ccaacagcat taatgttggt
    21601 tcaagcaagg acgattgctt aagaacctcg ctccagaata tcgccatttc tgcctgcggc
    21661 ataacgctgg cggcgttggc gtataccagc acggtaccga acagaccaaa agcgacacca
    21721 acagtacaaa taatgatgta cttccatgct gattccagcg atgaacgctg accgtaaatg
    21781 cctaccagaa acgccgagct taaggtggtg gcttcgatcg cagcccacat cacaatcagg
    21841 ttattgctgg taacaaccag cagcatggtg aacaaaaaca gatggaagaa accgtagtaa
    21901 tcgcacagcg taacgggtga aagctcgccg tgtgccactt catgacgcat gtagccaatc
    21961 gagtaaacac cggtgagaaa gccaatcaca ccaagaatgg cgaggaacaa accgcccaga
    22021 ccatcaatat gaagccacag tcccgcagcg aatatttctc ctgcatcagc ggcagtttgg
    22081 accacccaga gtgccagaat cagcagcagt gtgatcccta agctatgtaa tacagtcacc
    22141 gtacaagtcg cagagagccc ccgtttccgg caggcaaaac agagcagcga aaaaagcagc
    22201 ggcgtgagca ggagtaaagc gaacatcaca gaataactca tctcattatc ccttcagcgc
    22261 ggtcaagttg ttcacgtcca gcgtgccgtg ggtacgccat atttttcttg ccagtaacac
    22321 catcacaatg acggcgaaga tggcgtcggt agcgatacct atttccacca gttccggtgc
    22381 tcgccaggca agaagcgcca gcaccagatg ggagccgttt tccatcaggc agtaaccaaa
    22441 aatttgccgc aggatattgc gctggctgac aatgcacagc aggccaagca gaaaatgacc
    22501 taacgctacc gccagcgccg gtttcagccc ggtagccatc ggtagcttca cgggctgaac
    22561 gacaaatgcg caaagcagga caattaacgc cgcgagcagt gccatcattg ccggaccgaa
    22621 taacgctttt tccgggatgt tctggggaat atttcgtgca gcgtaagtca tgattaacgg
    22681 taccagcagc actttggtga taaaggcgct ggcggaccag atcagcagtt gctctgcggc
    22741 gaacaggcac gagagagtgg cgaaaataga caccagcacc agcgactggc aggcgtaaaa
    22801 tccgcatgac aggcgatagc ttttgacgct aatcacaaac agcgatgtca gcatcatcag
    22861 tcccgccaga ttatttacga tcatagaacc ggtcattcat catctcctta ctgcaaccag
    22921 gtcgcggcga ttacgctgga aatcagcgac atcacaatca gtacaatcaa caccaggcgc
    22981 attgaccctg gcagcggtgc ggcatcggcg acggcctcgc tcggtttgcc aggcacaacg
    23041 cgaccaaacc agcgaataaa ccaggcgaaa ctggcgaccg attcaatcat cagcagaatc
    23101 atggcgggca gcaggatcca gtactccact gacaacgcaa aaccggcagc aaacagcggg
    23161 aatttactga agaagccgtt gaacggcggc acgccggtaa tcgccagcgc tgccacgcag
    23221 aaacccacgc ctggcaacgg cagggtgtgc aatacgccac gcagacgcgg caacaagcgc
    23281 gtgccgcagc tgtaactcag cgcacctgct acaaggaaaa acaggctttt agcgaacgcg
    23341 tggttgacga tgtaggcgat gctaccctcc agcgccagcc gcgagccgaa gatggagagc
    23401 gacaagccga agaacatcca gccaagttga gtgatggtcg accaggccag caaccgcttc
    23461 atatcctgct gtggcaaata catcagaaag ccataaagaa tggtgaccag tgccatgacc
    23521 atgccaacgc cgccaatcac atgcgggata ttgccgccgt cgataattgc gcgggcaaaa
    23581 atgtaaacgc ccactttcac catcgatgcg gcgtggagat aggcgctgat cggtgttggc
    23641 gcttccattg catccggtag ccacgcttgc atcggtagct gggccgattt cccccacgcg
    23701 gcaaacagga tcccgccata aaccagataa cgtgcgtcgc cgtgtaactc gctcattgcg
    23761 ctaagcgcaa acgttccggt ctgcaaaaac agcgtggcgg cggcaagata caaccccaac
    23821 gaaccgatat gggtgataag caacgctttt agtgctgaac gctgcgcttt atcgctctgg
    23881 taataactga tcaacgccca ggagcagccg ccctaatttc aaaaaacaac aactgaccga
    23941 gcagcgtcga ggagagtacc agtcccgcca tcgcgccgat aaacaccagc aaaaatgcgt
    24001 aataacgatt cgtgccgtta tgcgggtgtt cgcgattttt atccgtcaga taacccgtgg
    24061 agtagatcgt gaccagcaaa ccgagaaaca ccaccacaaa cagaatcagc gtactcacgc
    24121 gatcaatgac caggccaaac aatgccacat cccccacgtt gaccaacgtc aacgtgacgg
    24181 caactttatc ggcctgataa aacgcggaga tcagcgacaa catgcacagc gtggtcagtg
    24241 cggcgaacaa aacccccatt cggcggcccg acgttgtggc gaaaacgaaa cgaccagtgc
    24301 gccgataaaa ggcagcatta acgtcgtcag agcaagattt tccatattcc gtcagtgctc
    24361 cttacagacc ggttaaccag aagacccatg caagcgcagc aaggctgaag ccaagccagg
    24421 tcacatggtg aatgagtaaa aaacgcccgc gtgccagcgt gttttctgcg attgaggcca
    24481 gtacaaaaat cagcaaaacc ttgagcagcg taacgacaag tgaagtcagc aggcaggcga
    24541 gagaaagttc ttgcgcgcgc ccaaagggca gaaacagggc cacaaacagt gctgccatca
    24601 cgacctgttt cagccccagc ccccatttcg ctagcgccag cccggcaccg gaatattcgg
    24661 tcagcgggcc ttcctgtaat tcctgttctg cttcagcaac atcaaaggga atttttccca
    24721 tctcaatgaa gcaggcaaaa ccacaggcca gtaacgccag tacggtggtt agcggcgagt
    24781 tccagcccgt tgccagcgta ttgctgatca tctcgatatg cgtggaacct gctatcagcg
    24841 ccaataccag cagtgagaga atgagcatgg gttcgaccag aatgccgagc gtcaactcgc
    24901 gactggcacc gactcccgca aacgggcttc cggtatccag cccggaaaga gcaaagaaaa
    24961 aacgaaacag ggcaagaaga tagataaggg tgatcagatc gccgccgccc gcaaaagggg
    25021 aaacggtaat aaaaagtggt agggccatcg ccagcaccag catgctgctg attaataccc
    25081 acggcatcag gcggaacatc agacctgaag atataggcgc aacttcctgg cgtttaaaca
    25141 gtttgtggat atcgcgataa tcctgccaga tccccggccc gcggcgggag tgcatacgcg
    25201 cgcgtatctg ccgggaaata cccgtaaaaa gcggggttag cagcagcaga atcacggcct
    25261 gtgctaacgc aaaaatgacc agatatccgt cgcaaagagt ttgtctcatg gtgatttcct
    25321 tagacggcaa tagcgattag cagcacaacc agtgcggcga ccacatacag gcaatagaga
    25381 cgaaagtcgc cgctttgcag atgctgtatt tctttggcca gccgttgggt ggcgctcacg
    25441 atggggcgga tcacccgctc atcccagaag ggttctgtgc tctgagccct ggcggtgacg
    25501 tgcgcaagat ctttatttag cctcagcgta gggtcgagct gttgtcgtag acgaaataac
    25561 gcagagaaga ccacacgcag cggctgcatc acgccattgc ctgacggagc catcgcgttt
    25621 tcccagccgt agccgcatgc ccaggcatct cctgtgcgac gaaacgctgc gcggcgcgaa
    25681 cgacaccaca gccagtaaag gccaggcagc aaaggcatcg ccagtagtaa cagcaacagt
    25741 aatgaaggag tgacccgtgt atgaaacgtg ccgggtacaa gtgctattcc gctggcgaca
    25801 gtgacgggag gggtattggt aaacgcatgg gcaatatgca ttatcttcgg tgccagccaa
    25861 ctggcactaa gcgcaattaa tacgcagagt gccgcgagca gtagcatggc gacgatcatc
    25921 gtatttggca cttcctgtgc ctcttcagcg tgtgtactgc gcggcgcacc acagaaagta
    25981 ataccgtaca ttttgacgaa gcacattact gccagcccac cagtgactgc cagcattacc
    26041 atagcaatag gacccgcaag ttgtagcgct acggcttcca cacgacttag tgagaacagc
    26101 gactgccagg tgtaccattc gctgataaaa ccattcagcg gaggaatggc tgatatcgcg
    26161 aggcaaccaa tcaggcatgc tgcggctgtc cacggcatcc gtttcgccag tgcccccatt
    26221 ttttccatgt cgtgggtatg caaacgcgaa ataatcgcgc ccgcgccgag aaatagcagc
    26281 cctttgaaca gcgcatggtt gagcagatga aacagtgcgc cgagcagtcc aaccacggtg
    26341 agcagcgggt cgtgcagtga cagaccgtcc atcgccacac cgactgccag caaaataatg
    26401 ccgacgtttt cgacggtact ccaggccagc agccgtttga tatcctgttc cgccagcgca
    26461 tatagcacgc ccaggagcgc ggagattgcg ccgatcgcca tcaccagaat gccccaccac
    26521 agaggcaaac ccgtttgcgc cagcagatcc atcgctactt tcaggatgcc gaaaataccg
    26581 attttgacca ttacgccaga catcaacgcc gaagcgtgcg atggtgcggc agggtgagcg
    26641 cgcggcaacc agctgtgcaa cggcatcatc ccggctttcg cgccaaaacc gaaaaaggcc
    26701 agcagaaaca ccgccgacgc cagccccgga gaaagtgaaa gcgtgcgaaa ctggcaaaat
    26761 cgaggctgcc gctttcgcgc cccatcagca agaaggcgat cataatcagc accgatccgg
    26821 cgtgggcgat aaaaaagtag agcatgccag cgttgatcga cgttttatcc tgcctggcaa
    26881 tgaccagaaa ccaggaagac agcgacatca tttcaaacag cacgatgaac caaaaagcgt
    26941 tgtccatcac cagcagggca accatcgatg cgatgaaaag attcataaag aagcccatcg
    27001 ccgccgcgcc tttgccctcg tattcgcgca tataagtcaa tgaatagagc gaacaaacca
    27061 ccaccagcaa ggagataacc agcaccataa atgccgagag gctatccatt cgcagggtta
    27121 aatcggcaaa gtcaaaaggg gtggcaaaac gggcgactaa agttacacca gcatgtaatt
    27181 gcgtaattcc gctgatgatg ccaatcacgc cgcccaccag cgatgtgatg ccggaaagct
    27241 taatagccag tctgtccaga ccgagtaaaa acagcgaagc cagactggca aacagataga
    27301 ggatcagcga ccaggttaat aattgcaggg catccattag tgttgctcct gagtgaggga
    27361 aaggggatcc tccccgccat tggcaaacca gcttgcggca aggcgctgtt tttctttcat
    27421 ctgacgttgc aggctatcgt cggtgatcag ccgtaacgcc tgattcgggc aagcgcgaac
    27481 gcaggccgga ccttctggca agaaatcaca caggtcgcat ttcaccgcga cggtctggac
    27541 acccggttcc cagcgcagca acgcatgttg gggcagcacg ttttcttcgc cgtctttgag
    27601 tgagccttca gcctgaaaaa catattgcgc atgggcattc accagacggc ttcctgaagc
    27661 gctgattgcg ccaaatgggc agaccacggc gcaaagcttg cagccaatac agaggctttc
    27721 gttgagttgg atcgcatcat ccctctgaga gatggcattg accgggcaga cctgcaggca
    27781 aggggcttcc tcacagtgat gacacacgac aggggcagtg attgttgacg tcttcgccag
    27841 ggccaggcgc gggtgttgct gtaaaccttg cgttttatgc acgtccgaac aggcagcgag
    27901 acaggtatta catcctgtac accacagtgg ttcggccacc acaaagcggt tcatgtcggc
    27961 tccttgaaaa tgatatgaaa cattgcattt catattgcat aaaggatgcc actctgatta
    28021 actcaatata ttcaataagt tatgtattga ctggattctg aacggtacaa aaatgatggt
    28081 cattaacgtt cagcaaaaaa tggattcgac acccgtgtcg caggggaaag cgagaatgtg
    28141 atcttgatca cgagaagtga ggtatgctgg ccgcaagcgc agccagcacg gaatggagca
    28201 aagtaatcag gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat tgctggtttt
    28261 gaaatcgtca aagacatgtt cgattttgcc atcagcgtca atcaggaagc tgatgcgatg
    28321 aatgccatcg taggttttgc ccatgaagga cttttcaccc cagacgccga attgttcgca
    28381 cacctggtgg tcctcatcag acaggagcgt aaagttaagc agctcttttt ccgcaaaacg
    28441 ggagagtttt tcgggtttat cggtgctgat acccagcaca tcaacgcccg cttttttcaa
    28501 ctcatccatg ttatcgcgta agccgcaggc ctgtacggta cagccggggg tcatggcttt
    28561 cgggtagaaa taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc
    28621 gtcttgatcc ggcaagctaa atttcggtgc gatatcaccg gctttcagtg gattcattac
    28681 ttaactccat cctgttcatc atgttgggaa taattgacga cgttaatact gccttgtgca
    28741 ttgagttctg tacatagggc tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg
    28801 gggctgtggg cggttatctg aatatgcaac tgcgcagccc gttcattttc agcaggttgc
    28861 gtgcgcgaca ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt
    28921 tcaattaaat gcggggagtc tgccacatcg acctgaaccc agacagatgc tggcattggc
    28981 ggacgcggac gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc ggcacctttc
    29041 aacggtaacg ttgattcaat cagagtaatg gcattccatg aaccggaaag cagcataata
    29101 aacgtgaact cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta
    29161 ctgacatgac gggtgatggt gttcacaatt ccagggcgat cggcacccaa cgcagtgatc
    29221 accagataat gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc
    29281 ttaaggaaag cataaaaaaa acatgcatac aacaatcaga acggttctgt ctgcttgctt
    29341 ttaatgccat accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc
    29401 acgggaagta ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct
    29461 agcttgaaaa aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt
    29521 ggcaccactg gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg
    29581 acgctggagc tggctgatgg gcgcattccg gtaattgccg ggaccggcgc taacgctact
    29641 gcggaagcca ttagcctgac gcagcgcttc aatgacagtg gtatcgtcgg ctgcctgacg
    29701 gtaacccctt actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc
    29761 gctgagcata ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat
    29821 ctgctcccgg aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag
    29881 gcaacaggga acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt
    29941 ctgctgagcg gcgatgatgc gagcgcgctg gacttcatgc aattgggcgg tcatggggtt
    30001 atttccgtta cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca
    30061 gaagggcatt ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa
    30121 ctatttgtcg aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg
    30181 gcgaccgata cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc
    30241 agagcggcgc ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc
    30301 tgttcaaaag tcgcgcctgg caaaggttgc gggtgtttcg cttgttttat tactcgctgc
    30361 ctgtagttct gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc
    30421 ggcaccgctt gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga
    30481 ttatgcaatc ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc
    30541 accagcccag ccgctggcac tggtttctgg cgcgcgtacc cagttcacgg gcgataccgc
    30601 ttcattgctg gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct
    30661 gcaggcgaaa aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccaccga
    30721 ttgggtacaa tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc
    30781 tgttaagccg cagggttatc agcaggcggt tacggttaaa ctgctgaacc tggaacaggc
    30841 gggcaaaccg gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt
    30901 tatttccgcc ggtctggata aatctgccac tgacgccgcg aacgctgcgc aaaatcgtgc
    30961 ctccaccact atggacgtac aaagtgcagc tgatgacacc ggtttaccaa tgctggtcgt
    31021 acgcgggccg ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat
    31081 gaaagtgacc gacagcaccc gttcgcaggg caacatggcc gtaacttata agccgctgtc
    31141 tgacagcgac tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa
    31201 actgcaggtt ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca
    31261 tactctgact cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa
    31321 gtaaaaatac agggctggaa tcatccggcc cttttttctg atatgatacg caaacgtgtg
    31381 cgtcggcagg aaaacgcgat tttagcggta attcgcacga aatttgtttg tcggacgtag
    31441 ttcggataag gcgttcacgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga
    31501 ttttaaaacg ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat
    31561 cgtggtaaag cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc
    31621 cgcaatgata cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg caaaggtatg
    31681 gtgaacaaca agttcaacta cttcattatg agcaaactgg ctgaagcggg tatcccgact
    31741 caaatggagc gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg
    31801 gttgagtgtg tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct tggaatcgaa
    31861 gaaggtattg agctgaaccc gccgctgttc gatctgttcc tgaaaaacga tgccatgcac
    31921 gatccgatgg tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg
    31981 gcgcgtatga aagagctgac ctacaaagcg aacgacgtgc tgaaaaaact gttcgatgat
    32041 gctggtctga ttctggtcga cttcaagctg gagtttggtc tgtacaaagg tgaagtggta
    32101 ctgggcgatg agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa
    32161 atggacaaag accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc
    32221 gcccgccgcc tgggtgtaca gctggactga tttttctggt tgcttgtttc tttagccgga
    32281 gggtactccg gctaaatgtc tttgataccc ccgctacaat acttctcatt atctcccttt
    32341 gacaggaaag tcataccagg ccaatatgta tatacataac gtatatccca aaaagggggg
    32401 gctatggaaa ctactgtatt tttaagtaat cgcagtcagg ccgtcagatt accgaaagcg
    32461 gtcgctctgc cagaagacgt taagaaagta gaaattattg cgattggtcg tacaagaatc
    32521 atcactcccg ccggtgagtc ctgggacagt tggtttgatg gcgaaaatgt gagtgcggat
    32581 tttatggata tccgtgatca acccgcaatg caggaaaggg aatcattttg atgctgaaat
    32641 tcatgctgga taccaatatc tgtattttca ctattaagaa taaaccagta catgtcagag
    32701 agcggtttaa cctgaattct ggccggatgt gtataagttc tgtaacattg atggagctta
    32761 tctatggggc agaaaaaagt cagatgccag aacgcaattt ggcggttatt gaaggtttcg
    32821 tatctcgttt ggtagtgctt gattatgata gtgctgctgc gacgcatacc gggcaaatca
    32881 gggccgagct tgcccggcag ggaaggcctg ttggaccttt tgaccagatg atagcgggac
    32941 atgctcgcag tcgcggtttg attgtggtta ccaacaatac ccgagagttt gagcgagtgg
    33001 cgggaatcag actagaagac tggagctaac attctctttt acttctaaca ttcgtaaatt
    33061 atataacgtg gcatgaagcc ttaattcctc cgccatttca tcccgctttc ctgtggtaat
    33121 cttcctctga accacctacg atcatttcta taatgaatat attgttgagg tatctatgcg
    33181 ttggcaaggg cgacgtgaaa gtgacaatgt tgaagacagg cgcaacagct ccggtggtcc
    33241 gtctatgggc ggtcccggtt ttcgcctgcc aagcggtaaa ggcgggctga ttttactgat
    33301 agtcgtgctg gttgcaggct actatggtgt tgatttaacc gggttaatga ctggacagcc
    33361 ggtttcccaa caacaatcaa ctcggtccat tagccctaat gaagacgaag ctgcaaaatt
    33421 cacctcggtg attctggcaa ccacggaaga cacctgggga caacagttcg agaagatggg
    33481 taagacctat cagcaaccga agctggtcat gtaccgtgga atgacgcgca ccggctgcgg
    33541 ggctggtcag tccattatgg ggccgttcta ttgcccggcg gatggcacag tttatatcga
    33601 tctctccttc tatgatgaca tgaaagacaa acttggcgcg gatggcgatt ttgcccaggg
    33661 gtacgttatc gcccatgaag tcggtcatca tgtgcagaaa ctgttaggca tcgagccgaa
    33721 agttcgtcaa ctgcaacaaa acgcgacgca ggcggaagtg aaccgcttat ctgtgcgtat
    33781 ggaactccag gccgactgtt ttgccggtgt ctgggggcat aacatgcagc agcaaggcgt
    33841 tctggaaacc ggcgatctgg aagaggcgct gaatgcggcg caggccatcg gcgatgaccg
    33901 tttacaacag caaagtcagg ggcgagtagt accagacagt ttcacacatg gcacttctca
    33961 gcaacgctac agctggttta aacgtggttt cgacagcggc gatccggcac aatgcaatac
    34021 ttttggtaaa agcatttaat ttccggggca gggatggctg aactgactgc gctttacaca
    34081 ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc tggtgttgag cggggaagag
    34141 ggttggtgtt ttgatcatgc gcttaagttg cgtgatgcct tacctggcga ctggctgtgg
    34201 atttcgccgc agccagatgc tgaaaaccac tgttcaccct cggcgctaca aactttactt
    34261 gggcgcgagt tccggcatgc ggtattcgac gcccgccacg gctttgatgc cgctgccttt
    34321 gcagcactta gcggaacgtt gaaagcggga agctggctgg ttttgttact ccctgtatgg
    34381 gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct ggagtgattg ccctgaccct
    34441 attgcgacgc cgcattttgt ccagcatctc aaacgcgtac ttacggcgga taacgacgct
    34501 atcctctggc ggcaaaacca gccgttctcg ttggcgcatt ttactccccg tactgactgg
    34561 caccccgcga ccggcgcacc acaaccagaa caacagcaac tcttacagca gctactgacc
    34621 atgcctccgg gcgtggcagc ggtaacggct gcgcgtgggc gcggtaagtc ggcgttggca
    34681 gggcaactca tttctcgtat tgcgggcagt gcgattgtca ccgcgcccgc aaaagcggca
    34741 acggatgtac tggcacaatt tgcgggcgag aagtttcgct ttattgcacc ggatgccttg
    34801 ttagccagcg atgagcaagc cgactggctg gtggtcgacg aagccgcagc catacctgcg
    34861 ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt tgttaaccac tacggtgcag
    34921 ggctacgaag gcaccggacg tggttttttg ctgaaatttt gcgctcgctt tccgcattta
    34981 caccgttttg aactgcaaca gtcgatccgc tgggcacagg gatgcccgct ggaaaaaatg
    35041 gttagtgagg cactggtttt tgacgatgaa aacttcaccc atacaccaca aggcaatatc
    35101 gtcatttccg catttgaaca aacgttatgg cgaagcgagc cagaaacgcc gttaaaggtt
    35161 tatcagttat tgtctggtgc gcactaccgg acttcgccgc tggatttacg ccggatgatg
    35221 gatgcaccag ggcaacattt tttacaggcg gctggcgaaa acgagattgc cggagcgctg
    35281 tggctggtgg atgagggggg attatctcaa caactcagtc aggcggtatg ggcaggtttt
    35341 cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg cgcacggtag caatccactg
    35401 gcagcgacat tgcgtggacg gcgggtcagc cggatagcag ttcatccggc gcgtcagcgg
    35461 gaaggcacag ggcagcaact tattgctggt gctttgcaat atacgcgtga cctcgactat
    35521 ctttcggtga gttttggtta caccggggag ttatggcgtt tctggcaccg ctgcggtttt
    35581 gtgctggtgc ggatgggtaa tcatcgtgaa gccagaagcg gttgctatac ggcgatggcg
    35641 ctgttaccga tgagtaatgc gggtaaacaa ctggctgaac gtgagcatta ccgtttacgt
    35701 cgcgatgcgc aagctctcgc aaagtggaat ggcgaaacgc ttcctgttga tccactaaac
    35761 gatgccgtcc tttctgacga cgactggctt gaactggccg gttttgcttt cgctcatcgt
    35821 ccgctattaa catcgttagg ttgcttattg cgtctgctac aaaccagtga actggcatta
    35881 ccggcgctgc gtgggcgttt acagaaaaac gtcagcgacg cgcagttatg taccacactt
    35941 aaactttcag gccgcaagat gttactggtc cgtcagcggg aagaggccgc gcaggcgctg
    36001 ttcgcactta atgatgttcg cactgagcgt ctgcgcgatc gcataacgca atggcaattt
    36061 tttcactgac tccttcaatt ttctcccatg gtcattgtga gtcagcggcg taaaattaat
    36121 gacatcagtt aaggagttcg ccatgaaaca tgaccatttt gttgttcaaa gcccggataa
    36181 acctgcgcaa cagttgttac tgcttttcca cggtgtcggg gataacccgg tggcaatggg
    36241 ggaaataggt agctggtttg caccgctgtt tcctgatgcg ctggtggtga gtgtcggtgg
    36301 cgcggagccg agcggtaatc cggcggggcg tcagtggttt tcggtgcagg gtattacgga
    36361 agataatcgc caggcgcgtg tggatgcgat catgccgacg tttattgaaa cggtgcgcta
    36421 ctggcagaaa cagagcggag taggggctaa cgccacggcg ctcatcggtt tctctcaagg
    36481 cgcgattatg gtgctggaga gcattaaagc cgaaccgggc cttgcttcac gcgtcattgc
    36541 ttttaacgga cggtatgcga gcctgccgga aactgcgtct acagccacga caattcacct
    36601 gattcatggc ggtgaagatc cggttatcga tctggcgcat gcggttgcgg cgcaggaagc
    36661 gttaatcagt gccggtggtg acgtgacgct ggatatcgtg gaggatttag gccatgcgat
    36721 cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt tataccattc cgaaacatta
    36781 tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat gatgtgattg aaatgatgta
    36841 aaagacagat tacctggcct gataagacgc gtgagcgtcg catcaggcat cgttcaccat
    36901 tgccggatgc ggcgttaacg ccttatccgg cctacggttg agcactcagc ttacttcttc
    36961 ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact tatcgttgaa atcacgatgc
    37021 gggggaagct ccggcttgtt ggcgagaaat ttcttatggt cgacgcgctt gagatcttta
    37081 atcacgttca gcaggacgcc taccaaaaag acaatcacca gaatccacca atatttagcc
    37141 agccagtcca tgcttatttc ctcttgcaga accactcatc aggcgacgag ctgttccatg
    37201 atacgttgat acatacgggc aagtagctgc aggtcggcag cgttcacaca ttcattaatt
    37261 ttatgaatag tggcattgac cggcccgagt tccaccacct gcgcccccat gcgagcaata
    37321 aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg gtttaatttc attatagtgc
    37381 tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg cggtcaaaaa tggctgcccg
    37441 gaaagccacc agtccaccgt ataacgcagt tgatgttttt caagcagggc aagcacctgt
    37501 gctttgatca tctcatcagt cagttcggtg ctgaagcgga agttaaactg cacaaacagt
    37561 tcacccggaa taacgttgtt actgcccgtt cccgcctgaa tattagcaat ctgcatactg
    37621 gtcgccggga aaaattcatt gccctgatcc cactcaatag ccactaattc attaaggaaa
    37681 ggggctgcgc gatgtactgg attgtcagcc agatgcgggt aggccacatg cccctgaacg
    37741 ccatgaatgg taaggttgca ggttaacgat ccgcgacgac catttttcac cacatcacca
    37801 accacttcga tactcgacgg ttcgccgacc aggcagtaat cgagacgctc attacgcgcc
    37861 attaacgctt cgacgacttt taccgtaccg ttgtgggcac tagcttcttc atcagaggtg
    37921 atcagaaatg ccagtcgccc cgtatggttg ggatgttgtg cgacaaaacg ttctgccgct
    37981 accaccatcg ccgccagcga gcctttcata tctgccgcac cgcgcccgaa taacatgccg
    38041 tcacgaatgg ttggttcaaa cggcggattg atccaacgat cggcgtcgcc aggcggcacc
    38101 acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc cacgccatgc ccaaaaattc
    38161 tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga tcgcctgcaa acgttcaatc
    38221 aacaaagcct ggcatcctgc gtcatcagga ctcagggaag ggcggcgaat aagctgttgt
    38281 gtcagctcaa taaccgggca cgacatagac tacacctcat ggaaaaattg ctgataactg
    38341 gaatcactga aacccagcag cataggctta ccgggcgcgc agagcaatgg acgtttgata
    38401 attgcaggca tttcagtcat taatgccgcc gcagaggccg catcggtgat tttattgcgg
    38461 gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt tgagtaacgc ttcccagcct
    38521 aattcgttga taaaaccgtt caataattcg ctgtccagcc catcgacgcg gtaatcatga
    38581 aaacgatagt cgatgttatt ggcttccagc caacggcgag cctttttaat ggtgtcacaa
    38641 tttttgatgc cgtaaagtgt aaccataatg aatccttttt cgatttatga gaataaatac
    38701 tcatttaagg gtgaaaacat tttgcctgca aatgattatc aggaaatata acattatcga
    38761 agtattcaaa ttaacaacta ttaaataata ttaaacattt ttaacgtaaa cattaagaat
    38821 attcatcttg ttatctgtct gtattttaac cagaattaaa tcagtattag atgccgtatg
    38881 aatatctcga aatgttgctg tgtatcacat acaaattatc accgcaggac catagtattt
    38941 acataaggtt tgtcccccgg aggtagtcat cgcagcaatc gagtaaatta gcttcaccac
    39001 ggtatattgt ttccgtagaa tacccataat tataagagag gttgttatga ttgaacgtga
    39061 actggggaac tggaaagact ttattgaagt tatgcttcgt aagtaattcc tgagacaaag
    39121 tacaaacagc aagaacccgc tgagcaggtt cttaatcgga aagttgaaaa aggcgacaca
    39181 ttagcatgtc gcctttttta ttgcttattc cgggcgcggc ttcagcggga agcggcggcg
    39241 caccagtaca aagaacagcg gcacgaagta aatagccaga atagtggccg aaatcatccc
    39301 gcccattacg ccagtaccca ccgcatgctg accaccggaa ccggcacccg tgctggttgc
    39361 cattggcaat acgccgaaga taaatgccag cgaggtcatc agaatcgggc gtaaacgctg
    39421 acggcaggcg tggagcgtcg cttcaaacag gtcgtggcct ttttggttca tctcgttagc
    39481 aaactcgacg atcaggatgg cgtttttcgc cgataaacca atgaccgtta acaggcccac
    39541 ctggaagtaa acgtcgtttt ccagcccgcg catccaggtt gccagcagcg cgccgataac
    39601 ccccagcggc acgaccagca ttaccgagaa cggtaccgac cagctttcat acaatgccgc
    39661 cagacacagg aatactacca gcaaggaaat ggcgtacagc gccggagcct gcgcgccgga
    39721 aagccgctcc tgatacgaca tcgccgtcca ctccagacca aagccgtttg gcaactgctt
    39781 cactaacgat tccataatat ccatcgccgt accggtactg acccccggtg cggcttcccc
    39841 aacaatctca accgcagaat aaccgttata gcgttccaga cgcggcgagc ctgtctccca
    39901 gcgtgaggtc gcgaaagcag agaagggcac catgccgcca tctttatttc ggacatacca
    39961 gagattaatg tcatctggca gcatgcgata cggcgcagct gcctgcacat agactttctt
    40021 cacgcgaccg cgatccataa agtcattcac atagctcgaa ccccaggcgg tttgcagtgt
    40081 gtcgttaata tcgtcgatag caacgcccag cgcctgagct ttacgctggt cgatatcaat
    40141 ctgcaactgc ggactgtcat cgaggccgtt atggcgcaca cgggttagct ccgggttttc
    40201 cgccgccagc gccagcaact ggttacgtgc tgccatcagc gcatcgtgac ctgctccagc
    40261 gtggtcctgc aactccatat caaaacctgc agaactacca agaccgctaa ttgctggcgg
    40321 gctgctggcg ataacgcgag cttctttaat cttgttaaac gccttcgttg cacgctcgat
    40381 aatggcaaat gaggtgccgg tcttactgtc gcgttcgctc cagtctttca ggcggataaa
    40441 cattcgcgcc acgttttgcc cgttaccccc ggggccagaa ccaacagtgg caaacaccga
    40501 catgatgttg tctttttcat gggtgaagta atatttctcg atttgctcaa cgactttcag
    40561 ggtctgttgt tgggttgaac cgctgggcaa ttgtaccgag gtagtaaaca tgccacggtc
    40621 ttccagcggt aagaacgacg tcggcaaacg caggaacagg aacaccatgc cgccaagcag
    40681 caggacataa atcacaatcc agcgcaggct acggtggaga attttcgcca cccctttttc
    40741 gtagcgttcg gcgttgcggt taaacatctg gttaaaccag gcaaaaaagc ctttttgccc
    40801 gtgatgctca ccttttttca gtggcttaag tagtgtggca cacagagccg gagtgaggat
    40861 catcgctacc agtactgaca gcaccatcgc cgcaacaatg gtaatagaga actggcgata
    40921 gatggcaccg gtggtgccgc cgaagaaggc cattggtaca aataccgccg aaagaaccat
    40981 cgcaatcccg accagtgccc cctggatctg ccccatcgat ttacgtgtgg cttcgcgagg
    41041 agtgagtcct tcctcactca taatacgttc gacgttttcc accaccacga ttgcgtcatc
    41101 caccagcaga ccgatcgcca gcaccatcgc gaacatggtt aaggtgttga cgctgtaacc
    41161 gaaggcgtaa agtacggaga aggttcccat caacaccacc ggcacggcga tagtgggtat
    41221 cagcgtggcg cggaagtttt gcaggaacag atacataacg aggaaaacca gagcgatagc
    41281 ttccagcagc gttttcacca cgtcttcaat ggaggcttta acaaacgagg tggtttcata
    41341 cgccaccttg tattccagtc cgtgcgggaa atactgcgcc agctcgtcga gacgattgag
    41401 caccagctcc gctgtcgcca tttcgtttgc gccggaggcc agttttaccc ccagcccgga
    41461 ggctggctta ccattgaagc ggctaagata atcgtatttc tccgccccca tttcgacggt
    41521 ggcgacatcg cccagcctta cctctgagcc gtcttgattc accctcaagg tgatatcgcg
    41581 gaactgttct ggtgtttgca gcagtgactg ggcgttaatg gtggcgttga gcgcctgctt
    41641 atcgacggaa ggtgtaccac caagttgccc aaccgcaatc tgcgcgttct gtgactcaat
    41701 ggcatcagtg acatctttag ccgtcatctg gaaactgttg agtttcgccg ggtccagcca
    41761 gatacgcatg gaatattgcg aaccataggc atcgatatcc ccgacgccat tcacgcggct
    41821 taacgggtcc tgaatattac tggcaacata atcggcaata tcctgtttat ccatcgaacc
    41881 atcggtagag acgaaagcaa tggtcagaat attggtatcg ccggttttac gcaccgtcac
    41941 gccctgattt tgcaccgcct gcggtaactt tcgcatggct gattgcagct ggttttgtac
    42001 ttgctgcacg gcttcgtccg gatcggtgcc tgctttaaaa cttaaagtga cagatgcctg
    42061 accggtgcca ctgctctgag atgacatata catgagatta tcgaggccgg tcatattttg
    42121 ctcgataacc tgggtcacgg tgttttccag cgtctgggcc gatgcgccgg gatagttagc
    42181 ggtcactcgc acattcggtg gcgcgagatc ggggtattgt tcaacgggca atgagaaaat
    42241 cgccagggta cctgtcagac acaacaggat tgccagcacc caggcaaaaa tggggcgatc
    42301 aataaagaaa ttcgccatta aaagaggacc tcgtgtttct gcgtatcgtt gtaggcttga
    42361 cttgcttcac tgtagcggca aggtacgagc caatcgtgga gaaaaaaagg agttaatgta
    42421 aattatcatg cccttttata ctgaacctta agtgcaagta ttctttggtc agtaggaggc
    42481 attacatcag ctgactttcc tcaccctccg cggagcgaaa gctaatactc actaacgtgc
    42541 cgccgccgga aggttgtgaa aaagtcagcg tcccacccag ccgttccgcg cgttcgcgca
    42601 tgatattcag accataatga ccttcgggct ctttcggttc accgatgcca atcccgttat
    42661 cacggatata aaccgtgtga ttgccgtccg gcgcggtgac gcaactgacg gcgatttcgc
    42721 tggcgttggc gtgcttcatc gcattcagca ccgcttcgcg aataatttgc aacaaatgca
    42781 cctgcatttg cgcatccagc gccagggttg gcagacggca gtcgagggtc agtttggcgc
    42841 tggtttgatt ttgtaacgta tccagcattt ccctcaacgc ggaggggaga tccgcctgct
    42901 gcagcgtcag gcggaaagta gtcaacagct cgcgtaactg ccgataagca tcattcaacg
    42961 cctgggaaaa atcggccatg atactttgtg cggtggcgtt atcttccggt atcgaacgct
    43021 tcagtaacgt caactggata cgtaagtaag aaagtacctg agccagcgag tcgtgcaatt
    43081 cgcgggcgat ggttgcacgt tcttccatca acaataactg ctgaaaatgc ttctgcgcct
    43141 gattaaagta caaaccgcgt cccagcatcg acgaaacgct gttaagcagc ggttctgatg
    43201 atgaaacgtg actattttgc cagtgcagtt cgccgtaaac cgtctcttgc attgtcaccg
    43261 gtaaaatctg catcggcaat tctgggtttg gttgcccttc gctaatccgc cagttttcac
    43321 cgacatttaa ctccagatat tcagccgctt cattgtcgcg aacaatctgc aaaatatggc
    43381 ggaaacaatg cacatcaatc tggctggtgt tcagcgcctg ggaacattga tacaacacct
    43441 ccagacgacg cttggcctcg tggagatcgc gggtcttttc ttctactgac gcctccagcg
    43501 aaaggtacaa tttatgcagc tcgctcgaca tctggttaaa ggtttttgca agcagaccaa
    43561 gctcattcgg cagactggta tccagcggcg gcgagtcgaa ctgcccgtgt tcaatacgtt
    43621 gactggcggt aaccagctga ttcagcgggg caaccacctg atggcgtata cggcgcaaag
    43681 taaaaaagac cagcgtgaaa ataccgatac cgccagccag ggaaatcgcc accaccagca
    43741 gcattttgcg ttcagcgtag tgctgtaaag ccagcacgaa caggtctatc tgattaacat
    43801 aattattaat attggcctga taccacggca aatcgccctt gctgagccga ttattcatct
    43861 ccagccagtt ggcattcaga tgcgcatagc gagtttttac tgcttctggc acataccaga
    43921 cgttgagatt ggttaatacc ggtgaatgca gtgcctgctg aaacaactgg cgatgtgcat
    43981 tgagttgtgg actgccactt tgcaagtcgt agcccaggcg gtaactctgc atacgtagcg
    44041 atccggcaat attgatagcc tcagcgtcgc gcaaactgct cgccagagtt agcagagcga
    44101 tacccgtgga aagaatcgac agcagcacaa tgtaaaaaaa ggcccgggcc agactggccg
    44161 agacgggtcg tttaacaatc acacgcgtct tctccacaaa aaatcattaa tgttccagtt
    44221 agctgaaaca caatttagtc agtgatggga ttatttgatc tgccacagtt tatagcgaaa
    44281 tagttactta ttctgggcac atgaacattg ccgattcccc tgtaagtcgg gtaataacaa
    44341 cactcatata aagaataagg tttttacaac caaaaaagaa ggtcgttatg aatcgtttta
    44401 ttatggccaa cagtcagcaa tgtctgggtt gtcatgcttg tgaaatcgcc tgtgtcatgg
    44461 ctcacaatga tgagcaacat gtcctgagcc aacaccattt tcatccccga attacggtta
    44521 tcaaacatca acagcaacgt agtgcagtga cctgtcacca ttgtgaagat gcgccctgcg
    44581 cccgtagctg ccctaatggc gcaatcagcc acgttgatga cagcattcag gtcaatcagc
    44641 aaaagtgtat tggctgtaaa tcctgcgtgg tggcctgtcc ttttggtacg atgcaaatcg
    44701 tcctgacacc cgtcgcggca ggaaaagtaa aagccacggc gcataaatgc gacctttgtg
    44761 cggggcgcga aaacggtcct gcctgtgttg agaattgccc ggcggacgcg ctgcaactgg
    44821 tcactgacgt cgcactctcc ggcatggcga aatcccgccg cttgcgcacc gcgcgtcagg
    44881 aacatcaacc gtggcatgcc agtaccgcgg cgcaagaaat gccggtaatg agtaaagtcg
    44941 aacaaatgca ggcaacgccc gcgcgtggcg agccggataa actggcgatt gaagcgcgca
    45001 aaaccggttt tgatgaaatt tatctgccat ttcgcgccga ccaggcacaa cgggaagcct
    45061 cgcgctgcct taagtgcggc gagcacagcg tttgtgaatg gacctgcccg ctgcataacc
    45121 atattccgca gtggattgaa ctggtgaaag ccggaaacat cgacgccgcc gtcgagcttt
    45181 ctcaccagac caacaccctg ccggaaatta ccggacgcgt ttgtccgcaa gaccgtttgt
    45241 gtgaaggtgc ctgtactatt cgcgatgagc acggtgctgt aactatcggc aacattgaac
    45301 gctatatttc agatcaggcg ttggcgaaag gttggcgtcc tgacttaagt catgtcacca
    45361 aagtggacaa gcgggtggcg attatcgggg caggcccggc agggctggcc tgtgcggatg
    45421 ttctgacccg caatggcgtg gcggtgacgg tgtacgatcg ccatccagaa attggtggct
    45481 tgctcacttt cggcattcct tctttcaaac tggataaatc cctgctggca cgccgtcggg
    45541 aaatcttcag cgcgatgggg attcacttcg aactcaattg tgaagtgggt aaagatgtct
    45601 ctttggattc gcttttggaa caatacgacg cggccttcgt tggcgtaggc acttaccgtt
    45661 ccatgaaagc gggtttaccc aatgaagatg cgccgggcgt ttatgacgcg ctgccgttcc
    45721 tcattgccaa cactaaacag gtgatggggc tcgaagagct accggaagag ccgtttatca
    45781 ataccgccgg acttaacgtc gtggtactgg gcggcggcga caccgcgatg gactgtgtgc
    45841 gtactgcgct gcgccacggc gcgagtaacg tcacctgcgc ttatcgtcgt gatgaagcta
    45901 acatgccagg ctcgaagaaa gaggtgagga acgcccgcga agagggggcc aacttcgaat
    45961 ttaacgtcca gccggtggcg cttgagctga atgaacaagg tcacgtctgc gggattcgtt
    46021 tcctgcgcac gcgtcttggc gagccggatg cccaggggcg tcggcgacct gtaccgattg
    46081 agggcagtga atttgtcatg ccagccgatg cggtgattat ggcgtttggc ttcaatccac
    46141 acgggatgcc gtggctggag tcgcacggtg taacggtaga caaatggggc cgcatcatcg
    46201 cggatgtgga aagccagtac cgttaccaga ccaccaatcc gaaaatcttc gccggtggcg
    46261 acgccgtgcg tggtgcggat ctggtggtta ccgcaatggc agaaggacgt catgcggcac
    46321 aggggattat tgactggctg ggggtaaaat cagtcaaatc tcactgatag cctgcgcaga
    46381 caaacccgac ttcacagcgt aagataattg ttcatttcgc gctgtggagt cggtatgacg
    46441 caacaaatca ccctcattaa agacaaaatt ctctccgata actatttcac cctgcacaac
    46501 attacctacg atctcacgcg caaagatggc gaagttatcc gccataaacg tgaagtttac
    46561 gatcgcggta atggtgcgac gatcctcctg tacaacgcga agaaaaagag cgtggttctg
    46621 attcgtcagt tccgtgtcgc tacctgggtt aatggcaatg aaagcgggca gctgattgaa
    46681 acctgcgccg ggctgctgga taacgacgaa ccggaagtgt gcattcgtaa agaagcgatt
    46741 gaagagacgg gctatgaagt gggcgaagtg cgcaaattat ttgaactgta tatgtcgcct
    46801 ggcggtgtga ctgagctaat ccactttttt atcgccgaat acagtgacaa tcagcgcgct
    46861 aacgccggtg gcggtgttga agatgaagat attgaagtgc tcgagctgcc gttcagccag
    46921 gcgctggaga tgatcaaaac cggcgagata cgtgacggta agacggtgtt attgcttaac
    46981 tatttgcaaa cgtcacattt aatggactga aaaataacaa taatatttcg ttgtttatta
    47041 ttggctcaat ccgacaaatc tgattgagcc gcgctacctg cgcaacgaag atacgacttg
    47101 tgctgtttgt ttgaacttct ggggtcgtac cgtccatgcg ctatcgcatt ttccttctct
    47161 tttttttcgc tttgttgccg acgtctttgg tgtgggcggc accagcgcaa cgggcgtttt
    47221 ccgactggca ggtcacctgt aataaccaaa acttctgcgt ggcgcgcaat acgggcgatc
    47281 ataatggact ggtgatgacc ctgagccgca gcgccggggc gcataccgat gccgttttac
    47341 gtattgagcg cggcgggttg aagtcgccgg atgcgtctga aggagagata gcgccacggc
    47401 tgctgttaga tggtgagccg ttggcgttaa gtggtgacaa gtggcggatt tcaccatggt
    47461 tattagtaac cgatgatacg gcaaccatca ccgcgttttt gcagatgatt caggaaggga
    47521 aggcgatcac cttacgcgac ggcaatcaga ccatttctct gagtggctta aaagcggcgt
    47581 tgttgtttat tgatgctcag caaaagcgcg ttggcagtga aaccgcgtgg atcaagaaag
    47641 gggacgaacc gccgctcagc gtaccgcccg cgcctgcgct gaaagaggtc gcggtggtta
    47701 acccaacgcc gacgccactc tcactcgaag aacgcaacga tttgctggat tatggaaact
    47761 ggcggatgaa tggtctgcgc tgctcgcttg atccgttacg tcgtgaggtg aatgtcactg
    47821 cgctgactga tgataaagcg ctgatgatga ttagctgtga ggcaggggcc tataacacca
    47881 ttgatttggc atggattgtg tcgcgtaaaa agccactagc ctcgcgcccg gttcggttgc
    47941 gtttgccgtt caacaacggt caggagacga atgaactgga actgatgaac gcaacatttg
    48001 atgagaaatc gcgtgaactg gtgaccttag cgaaagggcg cggattaagc gattgtggca
    48061 ttcaggcgcg ctggcgcttt gacggtcagc gttttcgcct ggtgcgttat gccgcagaac
    48121 ccacctgcga taactggcat gggccagacg cctggcccac gttgtggatc acccgttaaa
    48181 caaaattgcg gggacatgta ggccggataa gacgctgtag gccggataag acgcgtcagc
    48241 gtcgcatccg gcaatcagca tccggcgatc accatcaggc acctttcact cccagcacct
    48301 tatgcgcttt tgccacaata ttctcggcgg taaagccaaa gaacgggaac agcttatccg
    48361 ccggagcaga ttccccgtaa cccgtcatcc cgacaattgc ccctttcaga ccaacatact
    48421 tgtaccagta atcggcaata cctgcttcca ccgccacgcg agccgcaacg ttagaaggca
    48481 acaccgactc ccgatattcc tcatcctggg cgtcgaaaat atcggtcgag ggcagggaaa
    48541 ctacgcgtac attgcgacct tctcctgcta atttctctgc cgcttgcagg gtaatttcca
    48601 tctctgaacc ggtggcaatc agaataatat ctggcttacc gccgctgtct ttcagcacat
    48661 aaccgccacg agcaatctct ttaacctgat ccggcgtacg ttccacctgg gccagattct
    48721 gccttgagag gatcagtgcc gtcggtccgt tgtggcgctc aaccgccagc ttccagccca
    48781 ccgccgcttc cacctgatcg cacggtcgcc aggtgctgaa atttggcgtt aagcgcaggc
    48841 tggccagttg ctcaacagcc tggtgcgtcg gaccatcttc gcccaggcca attgagtcgt
    48901 gggtataaac cataatctgc cgcgctttca tcagtgccgc catccgcgcg gcgttacggg
    48961 cgtattcaac aaacatcagg aacgtcgcgg tatacggcac aaagccgccg tggtgcgcga
    49021 tgccgttggc gatagcggtc atgccaaatt cacgcacccc gtagtgaatg tagttgcccg
    49081 ctggatcttc cttcagcgaa acagaacctt tccagatggt caggttgctg ggagccagat
    49141 ccgccgaacc gccgagcaac tcaggcagca tcggcccgta agcgttaagc gtattttgcg
    49201 aagccttacg ggtagcgatt ttcgccggat ttgcctgtaa ctcattgata tatttctgag
    49261 tcgttttctc ccagtccttc ggtaaaccac cgctcatccg tcgggtaaac tcttctgcca
    49321 gttgcggatg agccttttta taggcggcaa acttctcatt ccagctctgc tgcgcttttt
    49381 cgcctttttc acgggcatcc caggcgtgat aaatcttttt agggatctca aatggcggat
    49441 ggtgccagcc cagtttttgc cgtgccagcg ccacttcttc ttcccccagt ggtgcgccgt
    49501 gcgcctcttc cttacctgct ttattcggcg aaccaaagcc aatcaccgta cggcagataa
    49561 tcagcgacgg cttatctttc acgctttgcg cttcaaggat cgcttccttc accgcctgcg
    49621 gatcgtgacc gtcgatttca tggatcacat gccagtgata ggcttcaaaa cgttttgccg
    49681 tatcgtcggt aaaccagcct tctgtttcac cgtcgatgga aataccgttg tgatcgtaaa
    49741 aaccaatcag cttgcccagt cccagcgtgc ctgccagcga acagacttcg tgggaaatac
    49801 cttccatcag gcagccgtcg cccataaaca cataggtgaa gtgatcgacg atctcatggt
    49861 ctggctggtt aaactgcgcc gccagtgtac gctctgctat cgccagcccg acggcgttcg
    49921 ccaaaccttg tccaagcggg ccggtggtgg tttcaacgcc tggcgtatag ccaatctccg
    49981 ggtggcctgg ggttttcgaa tgcaactgac ggaagttctt cagttcttcc agcggcaggt
    50041 cgtaaccggt cagatgtagc aaactgtaga gcagcatcga cgcgtgaccg ttggaaagaa
    50101 taaagcggtc gcgatcatac caggttgggt cggtaggatt atgtttaaga aaatcgttcc
    50161 acagcacttc ggcaatatca gccatgccca tcggcgcgcc gggatgacca gaattggctt
    50221 tttgtaccgc atccatactg agtgcgcgaa tcgcattggc aaggtctttt cgggacatat
    50281 aacactccgt ggctggttta tagtttggcg gcaagaagat cttccagttt gcgttgatca
    50341 acagcgaaca gacgaatgcc ttcagacagt ttttctaccg ccatcgcatc ctgattgtgc
    50401 tcccaacgga actccgcttc gctcatgggt gctgggcgtg ggaacgtctg agaaggcggg
    50461 attaatttac gtaccactgg cgaaactttt tcctgcagct ccttcagcaa attcggtgcg
    50521 atagtcagtc gatcgcagcc ggttaaggcg aggatttgtt cggtgcgacg gaagctcgcg
    50581 cccatcacaa tggtttcata gtggtgttgc ttatagtagt cgtagatatt gcgcaccgat
    50641 ttaacgcccg gatcttcttc caccacatac gggtccatcg gcttgcgtgc ctgataccag
    50701 tcataaatac gcccgacaaa cggcgaaatc agaaaaacgc ccgcttctgc acaggcccgc
    50761 gcctgggcaa aagaaaacag cagcgtcagg ttgcagttaa taccttcttt ttccagctct
    50821 tcggccgcgc gaattccttc ccaggtcgaa gccagtttga tcaaaatgcg tgatttctca
    50881 acgccttgtt gctgatacaa gtccaccaga tggcgcgcct tctcaatact cttttcttta
    50941 tcaaaagaga ggcgtgcatc aacttctgtt gacacgcgac cgggtacgat tttgagaatt
    51001 tcagcaccga aattgactgc cagtttgtca cacgctgcga ccacctgttg ttcctgggtc
    51061 ttgccatttt ttttacccca ggcgatagca tcgtctatta aatgctcata ttgtgataat
    51121 ccggcagctt tgagtaacag cgaaggattg gtggtggcat cctggggatg ataatggcga
    51181 atggactcaa tatcgccgct gtctgccacg acagtggtga actgtttgat gccgtctaac
    51241 tcgttcatag gaaatactcc ttgaaaagta aagtgttaga tgagtgcgtt aattcacact
    51301 tctgagaaat ttcgctaaac gcatcaaaaa agcatagcag acaggcatgg tattgctgga
    51361 ttaagcaggt aacatcagtg ttataggatt attaccaaaa cattatatta atggtgagag
    51421 tttggacttg ctcaaagtct gtagactccg gcagggtaat aatgtgcgcc acgttgtggg
    51481 caggggctgt tgcccacaca ctttatttgt gaacgttacg tgaaaggaac aaccaaatgg
    51541 atgaccagtt aaaacaaagt gcacttgatt tccatgaatt tccagttcca gggaaaatcc
    51601 aggtttctcc aaccaagcct ctggcaacgc agcgcgatct ggcgctggcc tactcaccag
    51661 gcgttgccgc accttgtctt gaaatcgaaa aagacccgtt aaaagcctac aaatataccg
    51721 cgcgcggtaa cctggtggcg gtgatctcta acggtacggc ggtgctgggg ttaggcaaca
    51781 ttggcgcgct ggctggcaaa ccggtgatgg aaggcaaggg cgttctgttt aagaaattcg
    51841 ccgggattga tgtatttgac attgaagttg atgaactcga cccggacaaa tttattgaag
    51901 ttgtcgctgc gctcgaaccg accttcggcg gaatcaacct cgaagacatt aaagcgccag
    51961 aatgtttcta tattgaacag aaactgcgcg agcggatgaa tattccggta ttccacgacg
    52021 atcagcacgg cacggcaatc atcagcactg ccgcaatcct caacggcttg cgcgtggtgg
    52081 agaaaaacat ctccgacgtg cggatggtgg tttccggcgc gggtgccgca gcaatcgcct
    52141 gtatgaacct gctggtagcg ctgggtctgc aaaaacataa catcgtggtt tgcgattcaa
    52201 aaggcgttat ctatcagggc cgtgagccaa acatggcgga aaccaaagcc gcatatgcgg
    52261 tggtggatga cggcaaacgt accctcgatg atgtgattga aggcgcggat attttccttg
    52321 gatgttccgg cccgaaagtg ctgacccagg aaatggtgaa gaaaatggct cgtgcgccaa
    52381 tgatcctagc gctggcgaac ccggaaccgg aaattctgcc gccgctggcg aaagaagtgc
    52441 gtccggatgc catcatttgc accggtcgtt ctgactatcc aaaccaggtc aacaacgtcc
    52501 tttgcttccc gtttatcttc cgtggcgcgc tggatgttgg cgcaacggct atcaatgaag
    52561 agatgaaact ggcggcggta cgcgcgattg cggaactggc ccacgccgaa cagagcgaag
    52621 tggtggcttc agcgtatggc gatcaggatc tgagctttgg tccggaatac atcattccaa
    52681 aaccgtttga tccgcgcttg atcgttaaga tcgctcctgc ggtcgctaaa gccgcgatgg
    52741 agtcgggcgt ggcgacgcgt ccgattgctg atttcgacgt ctacatcgac aagctgactg
    52801 agttcgttta caaaaccaac ctgtttatga agccgatttt ctcccaggct cgcaaagcgc
    52861 cgaagcgcgt tgttctgcca gaaggggaag aggcgcgcgt tctgcatgcc actcaggaac
    52921 tggtaacgct gggactggcg aaaccgatcc ttatcggtcg tccgaacgtg atcgaaatgc
    52981 gcattcagaa actgggcttg cagatcaaag cgggcgttga ttttgagatc gtcaataacg
    53041 aatccgatcc gcgctttaaa gagtactgga ccgaatactt ccagatcatg aagcgtcgcg
    53101 gtgtcactca ggaacaggcg cagcgggcgc tgatcagtaa cccgacagtg atcggcgcga
    53161 tcatggttca gcgtggggaa gccgatgcaa tgatctgcgg tacggtgggc gattatcatg
    53221 aacattttag cgtggtgaaa aatgtctttg gttatcgcga tggcgttcac accgcaggtg
    53281 caatgaacgc gctgctgctg ccgagtggta acacctttat tgccgatacc tacgtcaatg
    53341 acgaaccaga cgcagaagag ctggcggaga tcaccttgat ggcggcagaa actgtccgtc
    53401 gttttggtat tgagccgcgc gttgctctgt tgtcgcactc caactttggt tcttctgact
    53461 gcccgtcgtc gagcaaaatg cgtcaggcgc tggaactggt cagggatcgt gcgccagatc
    53521 tgatgattga tggtgaaatg cacggcgatg cagcgctggt ggaagcgatt cgcaacgacc
    53581 gtatgccgga cagccctttg aaaggttctg ccaatattct ggtgatgccg aacatggaag
    53641 ctgcccgcat tagttacaac ttactgcgcg tttccagctc ggaaggtgtg actgtcggtc
    53701 cggtgctgat gggcgtggcg aaaccggttc acgtgttaac gccgatcgca tcggtgcgtc
    53761 gtatcgtcaa catggtggcg ctggccgtgg ttgaagcgca aacccaaccg ctgtaattct
    53821 tttttaactc tcacgcttac cctgaatatt cagggtaagc ggtttggctg caatatatta
    53881 gtaaagctta ttactgagt
//
LOCUS       DAZKIV010000013        53172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZKIV010000013 DAZKIV010000000
VERSION     DAZKIV010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.013"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            318..1634
                     /gene="fucP"
                     /locus_tag="ACHOT6_000834"
     CDS             318..1634
                     /gene="fucP"
                     /locus_tag="ACHOT6_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFP9946096.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            1667..3442
                     /gene="fucI"
                     /locus_tag="ACHOT6_000835"
     CDS             1667..3442
                     /gene="fucI"
                     /locus_tag="ACHOT6_000835"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFP9946097.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            3551..4969
                     /gene="fucK"
                     /locus_tag="ACHOT6_000836"
     CDS             3551..4969
                     /gene="fucK"
                     /locus_tag="ACHOT6_000836"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFP9946098.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIERLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGAEQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            4971..5393
                     /gene="fucU"
                     /locus_tag="ACHOT6_000837"
     CDS             4971..5393
                     /gene="fucU"
                     /locus_tag="ACHOT6_000837"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFP9946099.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            5451..6182
                     /gene="fucR"
                     /locus_tag="ACHOT6_000838"
     CDS             5451..6182
                     /gene="fucR"
                     /locus_tag="ACHOT6_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFP9946100.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(6226..7326)
                     /gene="rlmM"
                     /locus_tag="ACHOT6_000839"
     CDS             complement(6226..7326)
                     /gene="rlmM"
                     /locus_tag="ACHOT6_000839"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFP9946101.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7319..7714)
                     /gene="ygdD"
                     /locus_tag="ACHOT6_000840"
     CDS             complement(7319..7714)
                     /gene="ygdD"
                     /locus_tag="ACHOT6_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFP9946102.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(7733..8650)
                     /gene="gcvA"
                     /locus_tag="ACHOT6_000841"
     CDS             complement(7733..8650)
                     /gene="gcvA"
                     /locus_tag="ACHOT6_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFP9946103.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKTLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(9002..9229)
                     /gene="ygdI"
                     /locus_tag="ACHOT6_000842"
     CDS             complement(9002..9229)
                     /gene="ygdI"
                     /locus_tag="ACHOT6_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFP9946104.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            9421..10626
                     /gene="csdA"
                     /locus_tag="ACHOT6_000843"
     CDS             9421..10626
                     /gene="csdA"
                     /locus_tag="ACHOT6_000843"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFP9946105.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSKLLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            10626..11069
                     /gene="csdE"
                     /locus_tag="ACHOT6_000844"
     CDS             10626..11069
                     /gene="csdE"
                     /locus_tag="ACHOT6_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFP9946106.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(11120..11926)
                     /gene="tcdA"
                     /locus_tag="ACHOT6_000845"
     CDS             complement(11120..11926)
                     /gene="tcdA"
                     /locus_tag="ACHOT6_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFP9946107.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(12165..13262)
                     /gene="mltA"
                     /locus_tag="ACHOT6_000846"
     CDS             complement(12165..13262)
                     /gene="mltA"
                     /locus_tag="ACHOT6_000846"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFP9946108.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            13471..13547
                     /locus_tag="ACHOT6_000847"
     tRNA            13471..13547
                     /locus_tag="ACHOT6_000847"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13505..13507,aa:Met,seq:cat)
     gene            complement(13622..14875)
                     /gene="amiC"
                     /locus_tag="ACHOT6_000848"
     CDS             complement(13622..14875)
                     /gene="amiC"
                     /locus_tag="ACHOT6_000848"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFP9946109.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            15107..16438
                     /gene="argA"
                     /locus_tag="ACHOT6_000849"
     CDS             15107..16438
                     /gene="argA"
                     /locus_tag="ACHOT6_000849"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFP9946110.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHQHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(16500..18326)
                     /gene="recD"
                     /locus_tag="ACHOT6_000850"
     CDS             complement(16500..18326)
                     /gene="recD"
                     /locus_tag="ACHOT6_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFP9946111.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(18326..21868)
                     /gene="recB"
                     /locus_tag="ACHOT6_000851"
     CDS             complement(18326..21868)
                     /gene="recB"
                     /locus_tag="ACHOT6_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFP9946112.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRDTVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARKIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVELAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVASVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWELVLTAWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(21861..24749)
                     /gene="ptrA"
                     /locus_tag="ACHOT6_000852"
     CDS             complement(21861..24749)
                     /gene="ptrA"
                     /locus_tag="ACHOT6_000852"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFP9946113.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIVLSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYSSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPEEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(24925..28293)
                     /gene="recC"
                     /locus_tag="ACHOT6_000853"
     CDS             complement(24925..28293)
                     /gene="recC"
                     /locus_tag="ACHOT6_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFP9946114.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKIYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMGAIVEQSQRFLLPLFRFNQS"
     gene            complement(28306..28629)
                     /gene="ppdC"
                     /locus_tag="ACHOT6_000854"
     CDS             complement(28306..28629)
                     /gene="ppdC"
                     /locus_tag="ACHOT6_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFP9946115.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPTNWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(28614..29021)
                     /locus_tag="ACHOT6_000855"
     CDS             complement(28614..29021)
                     /locus_tag="ACHOT6_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFP9946116.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVTMESQS
                     LRRQAIVQSALEWGKMHAWQVQQAVQCSQYAATGARACLRRLADNKTLLIAGYEDVSL
                     WRMGEVIDGNIVFSPRGWIDFCPLKEGALCQLP"
     gene            complement(29018..29581)
                     /gene="ppdB"
                     /locus_tag="ACHOT6_000856"
     CDS             complement(29018..29581)
                     /gene="ppdB"
                     /locus_tag="ACHOT6_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9946117.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(29572..30042)
                     /gene="ppdA"
                     /locus_tag="ACHOT6_000857"
     CDS             complement(29572..30042)
                     /gene="ppdA"
                     /locus_tag="ACHOT6_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFP9946118.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(30227..31021)
                     /gene="thyA"
                     /locus_tag="ACHOT6_000858"
     CDS             complement(30227..31021)
                     /gene="thyA"
                     /locus_tag="ACHOT6_000858"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFP9946119.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(31028..31903)
                     /gene="lgt"
                     /locus_tag="ACHOT6_000859"
     CDS             complement(31028..31903)
                     /gene="lgt"
                     /locus_tag="ACHOT6_000859"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFP9946120.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(32054..34300)
                     /gene="ptsP"
                     /locus_tag="ACHOT6_000860"
     CDS             complement(32054..34300)
                     /gene="ptsP"
                     /locus_tag="ACHOT6_000860"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9946121.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYADAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(34313..34843)
                     /gene="rppH"
                     /locus_tag="ACHOT6_000861"
     CDS             complement(34313..34843)
                     /gene="rppH"
                     /locus_tag="ACHOT6_000861"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFP9946122.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(35196..35342)
                     /gene="ygdT"
                     /locus_tag="ACHOT6_000862"
     CDS             complement(35196..35342)
                     /gene="ygdT"
                     /locus_tag="ACHOT6_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFP9946123.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            35528..36217
                     /gene="mutH"
                     /locus_tag="ACHOT6_000863"
     CDS             35528..36217
                     /gene="mutH"
                     /locus_tag="ACHOT6_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFP9946124.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            36286..36999
                     /gene="ygdQ"
                     /locus_tag="ACHOT6_000864"
     CDS             36286..36999
                     /gene="ygdQ"
                     /locus_tag="ACHOT6_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9946125.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            37137..37355
                     /gene="ygdR"
                     /locus_tag="ACHOT6_000865"
     CDS             37137..37355
                     /gene="ygdR"
                     /locus_tag="ACHOT6_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFP9946126.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            37463..38503
                     /gene="tas"
                     /locus_tag="ACHOT6_000866"
     CDS             37463..38503
                     /gene="tas"
                     /locus_tag="ACHOT6_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFP9946127.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(38535..39728)
                     /gene="lplT"
                     /locus_tag="ACHOT6_000867"
     CDS             complement(38535..39728)
                     /gene="lplT"
                     /locus_tag="ACHOT6_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFP9946128.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(39721..41880)
                     /gene="aas"
                     /locus_tag="ACHOT6_000868"
     CDS             complement(39721..41880)
                     /gene="aas"
                     /locus_tag="ACHOT6_000868"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFP9946129.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARLAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            42466..43497
                     /gene="galR"
                     /locus_tag="ACHOT6_000869"
     CDS             42466..43497
                     /gene="galR"
                     /locus_tag="ACHOT6_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFP9946130.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(43504..44766)
                     /gene="lysA"
                     /locus_tag="ACHOT6_000870"
     CDS             complement(43504..44766)
                     /gene="lysA"
                     /locus_tag="ACHOT6_000870"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFP9946131.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRNHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            44888..45823
                     /gene="lysR"
                     /locus_tag="ACHOT6_000871"
     CDS             44888..45823
                     /gene="lysR"
                     /locus_tag="ACHOT6_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFP9946132.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(45810..46502)
                     /gene="ygeA"
                     /locus_tag="ACHOT6_000872"
     CDS             complement(45810..46502)
                     /gene="ygeA"
                     /locus_tag="ACHOT6_000872"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFP9946133.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(46631..48049)
                     /gene="araE"
                     /locus_tag="ACHOT6_000873"
     CDS             complement(46631..48049)
                     /gene="araE"
                     /locus_tag="ACHOT6_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFP9946134.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(48364..49125)
                     /gene="kduD"
                     /locus_tag="ACHOT6_000874"
     CDS             complement(48364..49125)
                     /gene="kduD"
                     /locus_tag="ACHOT6_000874"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFP9946135.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(49155..49991)
                     /gene="kduI"
                     /locus_tag="ACHOT6_000875"
     CDS             complement(49155..49991)
                     /gene="kduI"
                     /locus_tag="ACHOT6_000875"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFP9946136.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(50278..51459)
                     /gene="yqeF"
                     /locus_tag="ACHOT6_000876"
     CDS             complement(50278..51459)
                     /gene="yqeF"
                     /locus_tag="ACHOT6_000876"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFP9946137.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            51713..52942
                     /gene="yqeG"
                     /locus_tag="ACHOT6_000877"
     CDS             51713..52942
                     /gene="yqeG"
                     /locus_tag="ACHOT6_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9946138.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNPNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT        13119 a        14326 c        13018 g        12709 t
ORIGIN      
        1 ctattcactc aagccaggaa tgcccgtttt cggcataaac aattaatacg gtcatctggt
       61 ttgtgttttt tatgatttat tttctgaaac gggcatgaaa tttcgattat taaagtgatg
      121 gtagtcacat aaagtcacct tccagctaat aagtgtgacc gccgtcatat tacagagcgt
      181 tttttatttg aaaatgaatc catgagttca tttcagacag gcaaatattc actgatatga
      241 agcccgaact cgctggtttt gcacttttga aaacataacc gattacgtgc ttaagcttct
      301 gaacctaaga ggatgctatg ggaaacacat caatacaaac gcagagttac cgtgcggtag
      361 ataaagatgc agggcaaagc agaagttaca ttattccatt cgcgctgctg tgctcactgt
      421 tttttctttg ggcggtagcc aataacctta acgacatttt attacctcaa ttccagcagg
      481 cttttacgct gacaaatttc caggctggcc tgatccaatc ggccttttac tttggttatt
      541 tcattatccc aatccctgct gggatattga tgaaaaaact cagttataaa gcagggatta
      601 ttaccggatt atttttatat gccttgggcg ctgcattatt ctggcccgct gcagaaataa
      661 tgaactacac cttattttta gttggcctat ttattattgc agccggatta ggttgtctgg
      721 aaactgccgc aaaccctttt gttacggtat tagggccgga aagcagtggt cacttccgct
      781 taaatcttgc gcaaacattt aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa
      841 gtcttatttt gtctaacgtg ccacatcaat cgcaagacgt tctcgataaa atgtctccag
      901 agcaattgag tgcgtataaa cacagcctgg tattatcggt acagacacct tatatgatca
      961 tcgtggctat cgtgttactg gtcgccctgc tgatcatgct gacgaaattc ccggcattgc
     1021 agagtgataa tcacagtgac gccaaacaag gatcgttctc cgcatcgctt tctcgcctgg
     1081 cgcgtattcg ccactggcgc tgggcagtat tagcgcaatt ctgctacgtt ggcgcacaaa
     1141 cggcctgctg gagctatttg attcgctacg ctgtagaaga aattccaggt atgactgcag
     1201 gctttgccgc taactattta accggaacca tggtgtgctt ctttattggt cgtttcaccg
     1261 gtacctggct catcagtcgc ttcgcaccac acaaagtcct ggccgcctac gcattaatcg
     1321 ctatggcact gtgcctgatc tcagccttcg ctggcggtca tgtgggctta atagccctga
     1381 ctttatgcag cgcctttatg tcgattcagt acccaacaat cttctcgctg ggcattaaga
     1441 atctcggcca ggacaccaaa tacggttcgt ccttcatcgt tatgaccatc attggcggcg
     1501 gtattgtcac tccggtcatg ggttttgtca gtgacgcggc gggcaacatc cccactgctg
     1561 aactgatccc cgcactctgc ttcgcggtca tctttatctt tgcccgtttc cgctctcaaa
     1621 cggcaactaa ctgaacatat tttccgaata aagtgaggaa tctgtaatga aaaaaatcag
     1681 cttaccgaaa attggtatcc gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc
     1741 gcttgaagaa caaacaatga atatggcgaa agctacggcc gcactgctga ccgagaaact
     1801 gcgccatgcc tgcggagctg ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat
     1861 ggctgaagcc gctgcttgcg aagaaaaatt cagcagtcag aatgtaggcc tcaccattac
     1921 ggtaacgcct tgctggtgct atggcagtga aaccatcgac atggatccaa cccgcccgaa
     1981 agccatttgg ggctttaacg gcactgaacg tcccggcgct gtttacctgg cagcggctct
     2041 ggcagctcac agccagaaag gcatcccggc attctccatt tacggtcatg acgttcagga
     2101 tgccgatgac acatcgattc ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc
     2161 cggtttggcc gtcgccagca tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat
     2221 gggtatcgcc ggttctattg ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt
     2281 ccaggcggtg gatatgaccg aactgcgtcg ccgtatcgat cagaagattt acgacgaagc
     2341 cgaactggaa atggcactga cctgggctga taaaaacttc cgctatggcg aagatgaaaa
     2401 taacaaacag tatcaacgca atgccgagca aagccgcgca gttctgcgcg aaagtttact
     2461 gatggcgatg tgtatccgcg acatgatgca aggcaacagc aaactggccg atattggtcg
     2521 cgtggaagaa tcacttggct acaacgccat cgctgcgggc ttccaggggc aacgtcactg
     2581 gaccgatcaa tatcccaatg gtgacaccgc cgaagcgatc ctcaacagtt catttgactg
     2641 gaatggcgtg cgcgaaccct ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc
     2701 aatgctaatg ggtcaccagc tcaccggcac cgctcaggta tttgccgatg tgcgtaccta
     2761 ctggtcacca gaagcaattg agcgtgtaac ggggcataaa ctcgatggac tggcagaaca
     2821 cggcatcatc catttgatca actcaggttc tgctgcgctg gacggttcct gtaaacaacg
     2881 cgacagcgaa ggtaacccga cgatgaagcc gcactgggaa atctctcagc aagaggctga
     2941 cgcttgcctc gccgctaccg aatggtgccc ggcgatccac gaatacttcc gtggcggcgg
     3001 ttactcttcc cgcttcctta ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat
     3061 catcaaaggc ctgggaccgg tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa
     3121 ggatgtgcat gacatcctca acaaacgcac caactcaacc tggccaacca cctggtttgc
     3181 accgcgcctc accggtaaag ggccgtttac ggatgtgtac tcggtaatgg cgaactgggg
     3241 cgctaaccat ggggttctga ccatcggcca cgttggcgct gactttatca ctctcgcctc
     3301 catgctgcgt atcccggtat gtatgcacaa cgttgaagag accaaagtgt atcgtccttc
     3361 tgcctgggct gcgcacggca tggatattga aggccaggat taccgcgctt gccagaacta
     3421 cggcccgttg tacaagcgtt aatacattac tccctgatgt gatgcccggt cgctccggct
     3481 accgggcctg aacaagcaag agtggttagc cggataagca atgttatccg gctatattgc
     3541 aggagcgatt atgaaacaag aagttatcct ggtactcgac tgtggcgcga ccaatgtcag
     3601 ggccatcgcg gttaatcggc agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag
     3661 cgatatcgcg atggaaaaca acacctggca ccagtggtct ttagacgcca ttttgcaacg
     3721 ctttgctgat tgctgtcggc aaatcaatag tgaactgact gaatgccaca tccgcggtat
     3781 cgccgtcacc acctttggtg tggatggcgc tctggtagat aagcaaggaa atctgctcta
     3841 tccgatcatt agctggaaat gtccgcgaac agcagcggtt atggacaata ttgaacggtt
     3901 aatctccgca cagcggttgc aggctatttc tggcgtcgga gcctttagtt tcaatacgtt
     3961 atataagttg gtgtggttga aagaaaatca tccacaactg ctggaacgcg cgcacgcctg
     4021 gctctttatt tcgtcgctga ttaaccaccg tttaaccggc gaattcacta ctgatatcac
     4081 gatggccgga accagccaga tgctggatat ccagcaacgc gatttcagtc cgcaaatttt
     4141 acaagccacc ggtattccac gccgactctt ccctcgtctg gtggaagcgg gtgaacagat
     4201 tggtacgcta cagaacagcg ccgcagcaat gctcggctta cccgttggca taccggtgat
     4261 ttccgcaggt cacgataccc agttcgccct ttttggcgct ggtgctgaac agaatgaacc
     4321 cgtgctctct tccggtacat gggaaatttt aatggttcgc agcgcccagg ttgatacttc
     4381 gctgttaagt cagtacgccg gttccacctg cgaactggat agccaggcag ggttgtataa
     4441 cccaggtatg caatggctgg catccggcgt gctggaatgg gtgagaaaac tgttctggac
     4501 ggctgaaaca ccctggcaaa tgttgattga agaggctcgt ctgatcgcgc ctggcgcgga
     4561 tggcgtgaaa atgcagtgtg atttattgtc gtgtcagaac gctggctggc aaggagtgac
     4621 gcttaatacc acgcgggggc atttctatcg cgcggcgctg gaagggttaa ctgcgcaatt
     4681 acagcgcaat ctacagatgc tggaaaaaat cgggcacttt aaggcctctg aattattgtt
     4741 agtcggtgga ggaagtcgca acacattgtg gaatcagatt aaagccaata tgcttgatat
     4801 tccggtaaaa gttctcgacg atgccgaaac gaccgtcgca ggagctgcgc tgttcggttg
     4861 gtatggcgta ggggaattta acagcccgga agaagcccgt gcacagattc attatcagta
     4921 ccgttatttc tacccgcaaa ctgaacctga atttatagag gaagtgtgaa atgctgaaaa
     4981 caatttcgcc gttaatttct cccgaactat tgaaagtgct ggcagagatg ggacatggag
     5041 atgaaattat tttttccgat gctcactttc ccgcccattc gatgggaccg caggtgatcc
     5101 gcgctgatgg cctgttggtg agcgacttgc tccaggcgat tatcccgtta tttgaactgg
     5161 acagttatgc accgccgctg gtgatgatgg cggcggtaga aggtgacact ctcgatcctg
     5221 aagtagaacg acgttaccgt aatgcgcttt cactacaagc cccgtgtcct gacatcatcc
     5281 gcatcaatcg ttttgcgttt tatgaacggg cgcaaaaagc ctttgcgatc gttatcacag
     5341 gcgaacgagc gaagtacggg aatattcttt taaaaaaagg ggtaacaccg taatctcata
     5401 ccggtacgcc cgcatgacgc gggcggttat cgaatgatgg ggtgaaaaat atgaaagcgg
     5461 cacgccagca agcgatagtc gacctgctgc tgaaccatac cagcctgacc acggaagctc
     5521 tctctgaaca gctaaaggtc agtaaagaaa ccattcgtcg cgatctcaat gaattacaga
     5581 cgcagggtaa aattctgcgc aatcatggac gcgctaaata tatccaccgt caaaatcaag
     5641 acagtggcga tccctttcac atcaggctga aaagccatta tgcgcataaa gcagatatcg
     5701 cgcgcgaggc gctcgcgtgg attgaagaag ggatggtgat agccttagac gccagttcaa
     5761 cttgctggta tctggcacgc cagttgcctg acatcaacat tcaggtcttc accaatagcc
     5821 atccgatttg ccatgaactc ggtaaacgcg aacgcattca actgatcagt tcaggcggca
     5881 cacttgagcg caaatatggc tgttacgtca atccctcgct gatttcccaa cttaaatcgc
     5941 tggaaatcga tctgtttatt ttttcttgtg aagggatcga tagcagcggc gcactgtggg
     6001 actccaatgc gatcaacgct gattacaaat cgatgctatt aaaacgtgcc gcgcaatcgt
     6061 tgttattgat tgataaaagt aaatttaatc gttcagggga agcccgcatc gggcatctgg
     6121 atgaggtaac gcacattatt tctgatgagc gccaggttgc aacttctttg gtaacagcct
     6181 gacggaaagg gtagcaggcc ggagacgacg ccccggcctt gcctgttatc gctcgtcgcg
     6241 acgaccaccc accgcagccc agatgcggcg gacgtgcacc gtcacttctt cgcgatcgtg
     6301 atacaactgc cgcgcctgaa tctgagcatt tatgccatgt tcatcaagct gtgcctgaat
     6361 atacgccaga ttgtgtgaca cttcttcgta gcgttttttc atcggcagtt tgaggttgaa
     6421 aatggtttca cggcaccagc cattaaccag ccactgcgcc atcaatgccg caactttcgc
     6481 cggtttttca accatatcgc ataccatcca ggagatattg ctgcgcgtcg gacggaattt
     6541 gaaaccgtct tcccgcagcc acgtcacctg tccggtatcc atcagacttt gcgccatcgg
     6601 gccgttgtcg acggaataaa cccacatgtt gcgcttcacc agttggtagg tccagccgcc
     6661 agggcaagcg cctaaatcca ccgcccacat cccgttcgcc aggcgttcat cccactcatc
     6721 cgcaggaata aacacatgaa atgcctcttc cagtttgagc gtggaacgac tcggcgcatc
     6781 tgccggaaat ttcaggcgcg gaatgcccat atagaacggc gaattattgt tgctgtatga
     6841 gtaaccggta tagcagcagc ctggtgcaat aaagaataca tgcacaaccg gacgcttcgg
     6901 cgtttcatag ttcgccagca ccccggcatc gcgcaaggca gcgcgtagcg gcacggtgaa
     6961 tttacggcag aatttcagta actctttgct ttcgttggta tcggcaactt caacacgcag
     7021 ttcaccgccc ttctctacta cgccctgtaa catgccgaca atgggggtaa tacgatcttc
     7081 tggcggcaaa tgctgcagga gttcccccac cacaaaccac tggcgggcaa aaattaatga
     7141 actgaacggc agctcacgga ttaacttatc gccatcatca ggttgataac attcataaat
     7201 gacataaccc gcattctctt tcacgcgggc aaaaccgaaa atttcccgct ggccggcttt
     7261 atcggtaatt tctgcggcgc actctttttc aaagcccgga cggcacagca atacaacctt
     7321 attcatgact tacgcccttg cgctttaaac ggatagcacc aactaacatt aacgcccagc
     7381 ccgcgaggaa gctcacgccg ccaaccggag tgacaaacgc ccacaaacgc agatgggaca
     7441 gcgccaggca ataaaggctg ccgctgaaca acaccgtgcc taacgcgagg aaaacgctac
     7501 tccagtaaaa ccagatactg atgcgacgct gcattgccac cgccagacct aagatcgcca
     7561 gcgtatgaaa cgcctggtat tcgaggccgg tctggatcca gcccatctca acggccccca
     7621 tggttttact taacacatgc gcgccaaaag cgcccagagc cacaaaaatg aagccgctaa
     7681 tggcggcgaa aatcagcata aaacggctgg tcatggtcgt accctacgta aattattgtt
     7741 cataacgaaa gcggaatttt tcttgttcag cagcggcttt cgccaggatc cattggcgaa
     7801 aggcggctat tttacccagt tctgcctggc tgtcatgaca aaccagataa aaagcgtttt
     7861 tactgaccag aacatcatta aacgggcaaa caagacgtcc ggcctcgatt tcagattgcg
     7921 ccatcacgtt atttgccagc gccactccct gcccgtgaat agccgcttgc agcaccatgg
     7981 cgctatgact aaaaattggc ccttgctgaa cgttgatatg atttaacccc aactgtcggg
     8041 tatatgtctg ccagtcacgg cgtgacgcat catgtaataa cgtatgttta gccagatctt
     8101 ccggggtctt caaggttttt tcgcctgtca gcagtagcgg cgaacacacc ggcaataaat
     8161 attcggcgta cagtttttcc acccgtagcc ccggccagtt gccccgacca taaaatatcg
     8221 ccacatcaac atcatccgcc agcttatctt cctgacgatc aaccgcctgg attcgaacgt
     8281 caattcccgg ataagctgaa ttaaagctcg aaagtcgcgg aaccaaccaa tgaatggcga
     8341 aactggggag taaactgacc gtcaacgccc ccttggcgct acgggcctgg agtttacgcg
     8401 tcgcttcggt taattgcgaa aatatctctt tgatatcgag gaaatagctt tgcccttctt
     8461 cggtcagcag gagtgaacga ttacggcggc ggaacagttt tagccccaaa aaatcctcaa
     8521 gagacttgat ttgatgactt actgcggcct gggtcacaaa aagctcttct gctgcgcgag
     8581 tgaaacttaa atggcgtgct gcggcatcaa aaactcgtaa ggcatttagc ggtggtaatc
     8641 gtttagacat ggcttttaaa ctttgatgtt aaatgaattt aacaattaga tcacactgtg
     8701 taacctatta gtttttttaa tctgagccat tataaattgt ccgttgagct tctaccagca
     8761 aatacctata gtggcggcac ttcctgagcc ggaacgaaaa gttttatcgg aatgcgtgtt
     8821 ctggtgaact tttggcttac ggttgtgatg ttgtgttgtt gtgtttgcaa ttggtctgcg
     8881 attcagacca tggtagcaaa gctacctttt ttcacttcct gtacatttac cctgtctgtc
     8941 catagtgatt aatgtagcac cgcctaattg cggtgctttt tttttacctt gcgatcgcga
     9001 attactgatc cagttcgacc atctctttca cgtcagtacg gttgatctgc tgtttgttgc
     9061 cattagcgtc tttatacgaa atcataccgg tatcgttatc agtctgtggt ttgccgtcag
     9121 agacgatggt acgtccgtca ttggtgtgca tcacatagtt cgaaccggaa caggcgctca
     9181 gggcaaaagt cagcatacag gcagaaataa ttgcggcagt ctttttcatc atcttctcct
     9241 taaagcgatt aataaatctt aatagtattg attaacaggc taaaattaac gcctaacact
     9301 attcagcata tgttacttac gcggcttcgc caggatatcc agataattct gatggttagc
     9361 actctccttg tatcaaagtg aattttgcgt cacgatcggt gcatcaagcc gaggagtacc
     9421 atgaacgttt ttaatcccgc gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc
     9481 gtctatctcg atagcgccgc gaccgcgctt aaacctgaag ccgtggttga agccactcga
     9541 cagttttaca gcctgagcgc cggaaacgtc catcgcagcc agtttgccga agcccaacgc
     9601 ctgaccgcgc gttatgaagc tgcacgagag aaagtggcgc aattactgaa tgcaccggat
     9661 gataaaacta tcgtctggac gcgcggcacc actgaatcca tcaacatggt ggcacaatgc
     9721 tatgcgcgtc cgcgtctgca accgggcgat gagattattg tcagcgtggc agaacaccac
     9781 gctaacctcg tcccctggct gatggtcgcc caacaaactg gagccaaagt ggtgaaattg
     9841 ccgcttaatg cgcagcgact gccggatgtc gatttgttgc cagaactgat tactccccgt
     9901 agtcggattc tggcgttggg tcagatgtcg aacgttactg gcggttgccc ggatctggcg
     9961 cgagcgatta cctttgctca ttcagccggg atggtggtga tggttgatgg tgctcagggg
    10021 gcggtgcatt tccccgcaga tgttcagcaa ctggatatcg atttctatgc tttctctggt
    10081 cacaaactgt atggcccgac gggtatcggc gtgctgtatg gcaaatcaaa actgctggaa
    10141 gcgatgtcgc cctggctggg tggcggcaaa atgattcacg aagtgagttt tgacggcttc
    10201 acgactcaat ctgcgccgtg gaaactggaa gcaggaacgc caaatgtggc tggtgtcata
    10261 ggattaagcg cggcgctgga atggctggca gattacgata tcaaccaggc cgaaagctgg
    10321 agccgtagtt tagcaacgct ggcggaagat gcgctggcga aacgtccagg ctttcgttca
    10381 ttccgctgcc aagattccag cctgctggcc tttgattttg ctggcgttca tcacagcgat
    10441 atggtgacgc tgctggcgga gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag
    10501 ccgctactgg cagaattagg cgtaaccggc acactgcgcg cctcttttgc gccatataat
    10561 acaaagagtg atgtggatgc gctggtgaat gccgttgacc gcgcgctgga attattggtg
    10621 gattaatgac gaacccgcaa ttcgccggac atccgttcgg cacaaccgta accgcagaaa
    10681 cgttacgcaa taccttcgca ccgttgacgc aatgggaaga taaatatcgc cagttgatca
    10741 tgctggggaa acagcttccg gctttgccag acgagttaaa agcgcaggct aaagagattg
    10801 ccggatgcga aaaccgcgtc tggctgggat ataccgtggc tgaaaacggt aaaatgcact
    10861 tctttggcga cagcgaagga cgtatagtgc gcggcctgct ggcggtgctg ttgactgccg
    10921 ttgaggggaa aaccgccgcc gaattgcagg cacagtcgcc gctggcattg tttgatgagc
    10981 tgggattacg tgcgcagctt agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg
    11041 cgattatcgc tgcgacgaag caggtttaag cacagaaccg ggtcggataa gacgttcgcg
    11101 tcgcatccga cctgattgtt taaccctgac gcgccgcttt cgccatcatc ttcttcagcg
    11161 catgagaaac cgcaacaaaa ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag
    11221 atgcacaatc catccgcttt ggcccttccg ccgtggcttt cattgcacac accgtaccgt
    11281 ctgactgcgg gtacaccagc gcttcagtag aaaacacgca atccacgccg agcttacctt
    11341 tactgttttt cactacgcca aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg
    11401 ggtcctgaat cgttttcgcc agatcggtaa cctgaatctg cgtcgggtca atttgcccac
    11461 ccgcgccacc ggtcgtgacc agcgggattt tattgcgccg acaataagca atcagcgccg
    11521 ctttgggccg tacactatca atggcatcaa tcacgtacga ataaccggca ctcatatatt
    11581 gcgctacgtt atccggcgtc acgaaatcat ccaccaccgt tacacggcac tccgggttaa
    11641 tctggcgaat acgttccgcc atgacttccg cttttgccag cccgacgtta tcacgcagag
    11701 cgtgaatttg ccgattggta ttggtgacgc acacatcatc catatcgata agcgtgattg
    11761 cgccaatccc cgtgcgcgcc agcgcttccg ccgcccagga accgacaccg ccaataccga
    11821 ccacacaaat atgcgcgtca gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg
    11881 tgccaccaaa acgctgacgc catgcatcac taattaccac agacatagca cctcagaatt
    11941 aaaaagggcg aggttatccc tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc
    12001 aggcctacat aactcctgca atattttgga tttgcatgat ttgtaggccg gataaggcgt
    12061 tcacgccgca tccggcatga acaacgcgca cttagtcaat aatcaggatg tgaggttacc
    12121 cctcaccctg tcatatccgt aaaaacggca tacagaatat cacatcagcc gctaaagacg
    12181 ttacctgcgc ccggggcggt tttcagcacc cagacacgtc catagtggtt ataccaacct
    12241 gcgcggtgtc cggcttccgg cccgatccct tgatagatat cgaagtgttg gcctttgatt
    12301 gcgccaccga catccagcgc caccatcaga cgcaattcgt actgaccgtt aaatttgccg
    12361 ttattatcca gcagcggcac ttctgccagc aaggttgtac ctggcggaat aattgaacga
    12421 tcggaggcaa cagaagcacg gccaaccagc ggcaccgcac ttgccccttt caccggtgca
    12481 aaagattgcg gtttaaagaa aacgaaagac gggttctgtt ccagcagctc gcgaacctcg
    12541 gcttcactgt gtgtttcgcc ccagtgacga atcgcctgca tcgacatatc ttcttttttc
    12601 acttcgccac ggtcgatcag caccttacca atgctgcgat aggcatgacc gtttttccct
    12661 gcatagctga aaaagttaag cggactgcca tcaccaaagt cgatataccc actaccctgc
    12721 acatccataa tgaagttatc catcagggag ttactgtaag cgagaatata tttatcactc
    12781 aatgcccccg cgtagatctc cgcacgagac ggcagacgac cacgttttgg cggcatacgg
    12841 taaataggat actggaactc gccctggcgg gtatggcgcg cctgaattac cggcgtgtaa
    12901 tagcctgtaa actgcacgtt accatagttg tcggcacctt ccatctgcca ggcatcaatg
    12961 ccgaactggc gcatattgcg ggtatcaccg cctgcgcgca gccactcttg caccgcgtta
    13021 taaacattac tctggttgcc atacagacgc ggtgacgaat tacggatatg gttaatttgc
    13081 tcggcaaaat caccggcgtt aatcggcgcg ccaacggcat ctggctgatt caccagagag
    13141 aaaggctggg taaatttccc gtctttatat tgctgtccgc gatcggttgg tttggaagag
    13201 caggcggcaa gcattgccac aaccgtgccc ataagaaggt actttaccca acgtcctttc
    13261 attgttctct tcttcaggtt aaaaaataag gcgcaacgaa gataacaaac cgccgcgttt
    13321 aatgaaatga gcagcctctc cctgacgcaa attttgtaca aaaaacaggc tttagtgatt
    13381 tgtttttgtt caaaatcatg ccagatccgt gatcggggta aaaaaaaggt tgcatgaaaa
    13441 cgcgagcgga gtatagtgcg cttccacgga cgcggggtgg agcagcctgg tagctcgtcg
    13501 ggctcataac ccgaaggtcg tcggttcaaa tccggccccc gcaaccaatt attgaacacc
    13561 ctaacgggtg tttttttgtt tctggtctcc cataaaaaaa gcgccattca gcgccttttt
    13621 atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc cccgcaagaa
    13681 tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct tcctcaacgt
    13741 tactgataaa cgccgtttcg accagaatgg agggaatatc aggcgctttc agcacggcaa
    13801 acccggcctg ttcaacctga tttttatgca gcttgttgat tttaccgagc ttattcagca
    13861 ccgctttacc aaacttcagg ctgtcggcaa tggtcagcga ctgcaccata tcgaacatgg
    13921 tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcg gaggcgttct
    13981 gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag agcgcaaaca
    14041 cagaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag acaaacaggt
    14101 cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag atgtcttcat
    14161 tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc aaacggcgag
    14221 ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca ccggagtctt
    14281 cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct gctttacctg
    14341 gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg ttgtaatcct
    14401 ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag agatccatca
    14461 ccagacgctc tttaaacccg gcgaccggcg caagtgcaaa cagctgcggt tttacgtttt
    14521 gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg cgcgccgact
    14581 tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc gagttcaggt
    14641 ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac tgcttatatt
    14701 tcagctgacg atttgattcc accgtcacgc gggtgtagct ggacgcaggc cagacgcgca
    14761 ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat agccacatgg
    14821 cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct gacatgcctc
    14881 tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga ccggaaactt
    14941 tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt atattcacgg
    15001 cattactgat aaaaaagtcg ctctcgcata aaattcacac ttgcaccctg cgaaaaaaca
    15061 gaataaaaat acaataattt cgaataatca tgcaaagagg tgtgccgtgg taaaggaacg
    15121 taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata cccaccgggg
    15181 aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt tctccagtat
    15241 cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg tctatggcgc
    15301 acgtccgcag atcgacgcaa atctggctgc gcatcaacac gaaccgctgt atcacaagaa
    15361 tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg gaacattgca
    15421 actggatatt accgctcgcc tgtcgatgag tctcaataac acgccgctgc agggcgcgca
    15481 tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg atgacggcgt
    15541 ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc atcgtcaact
    15601 ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg gcgagagctt
    15661 taatctgacc tcggaagaga ttgccactca actggccatc aaactgaaag ctgaaaagat
    15721 gattggtttt tgctcttccc agggcgtcac taatgacgac ggtgatattg tctccgaact
    15781 tttccctaac gaagcgcaag cgcgggtaga agcccaggaa gagaaaggcg attacaactc
    15841 cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg tgcgtcgctg
    15901 tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct cacgcgacgg
    15961 tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa caatcaacga
    16021 tatcggcggt attctggagt tgattcgccc gctggagcag caaggtattc tggtacgccg
    16081 ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc gcgataacac
    16141 gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg aaatggcctg
    16201 tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc tggaacgcat
    16261 tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca cgcgcagtat
    16321 tcactggttc caggaacgtg gatttacccc agtggatatt gatttactgc ccgagagcaa
    16381 aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt tagggtaagc
    16441 gatgaaaatc gccggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg caattacgtt
    16501 tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg agtggcgatt
    16561 gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc gcgggtcacc
    16621 gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa aatcaacgcc
    16681 gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt agtttcgtgc
    16741 tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc aaaccagacg
    16801 cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa cccaagcgcg
    16861 ctgtcattac gggcaatcat caccggtcga ccttcgtacc aacgagagtg cggatgacga
    16921 tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc agccacgcca
    16981 aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg aatgattaaa
    17041 tccggctcgg cacgcgcttg cagcagatcc agataacgtc cataacccgc aagagcttcc
    17101 tcaagcatcg caatataatc ttcgccgctc tgtaaaagcc gtttttcgat atcagtaaaa
    17161 tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttaatcgc cgcagctaac
    17221 tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca gagactgtcg
    17281 cgcaaagatg ccgcttctgt gccagttcct gccggaacgt gagttcccgt caggcggctt
    17341 agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat atcgcccagc
    17401 acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat cactcgcgca
    17461 tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat tgacgcttca
    17521 tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa acgctggcta
    17581 cccggctgcg cacccagcaa tcggtgcaaa gtactggcat cttccggaat gcgtttcttt
    17641 tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt taagcgcgcg
    17701 gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc ggccatttga
    17761 attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg gccgccggaa
    17821 atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca gttaatttca
    17881 tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc aacctcaatg
    17941 gcatgattca cttcgttgaa aaagcgtgcc actgtgcgtt cgttacacca catgcgattc
    18001 aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctccccg gctgaccgct
    18061 tgtgaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact gacacaggtc
    18121 gccaacagcg gatgcgacgc ctcattattt tccagtcgtg aaagcggcaa acagacgtgt
    18181 ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg atgttcatct
    18241 cccgccacag tcagggcaaa ctgcacgtcc agcgggcgta gctgtttgtg ctccacagct
    18301 tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg gcaaacatct
    18361 catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatcccc tgttgtggat
    18421 gttctttatc aacgccacgc aggaacagat aaataacgcc gccaaagtgg cgctcatagt
    18481 cgtaatcagc aatgcgatga cgcagataac gatgcagcgc cagggtataa agctgatatt
    18541 gcagatcata gcggtgtgcc tgcatcgccg ctgccatagc ctgttgggtg taagccgaac
    18601 tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct tcgtggcgga
    18661 acaccaggtc gataaagcct tttaacatgc cacgtacctg catgaactcc agcggcgggc
    18721 agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg gcgatgagcg
    18781 gttcgctaat cggcaggtaa aactccatct ctacctgttt atcgcggtcg gaaagctgac
    18841 tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc cacgcggtca
    18901 acaccagttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt acccagttcg
    18961 ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat gtccccggtg
    19021 acgcaccgcg cgggaactga tgaggtgtta acgtcggttc ttcaacgacg ctggcaacgc
    19081 ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga ccacgctgct
    19141 gcaaaccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt agcgtcctcg
    19201 cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca ccagtttgtg
    19261 ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga agccctgccg
    19321 catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg acgtcggtgt
    19381 cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg caatgccaaa
    19441 ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt tcgacctccg
    19501 caagctcgac gctttctggc gcagcgttaa gatccagaac agcctcaaac gagtggcgat
    19561 cgtgataaaa cgcctgatcc tggacgcgga aattggtgat aaacggcagc cagaccagcg
    19621 gatattccag cccttttgat ttatggatcg tgacaatctg taccagatgt ttatcacttt
    19681 cgaggcgcat ttgttggcta gaggcattac tgtctggctc gaggatatgt tgcgataacc
    19741 agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc agttcgctga
    19801 tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag ttttctgcaa
    19861 tcttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg cgccagattt
    19921 gccgataacc atcgaactct tcgaccaccg cgtcccacgc atgttcgtca ttattcagcg
    19981 tttcgatatc cagcgcgttc agccccatca ttgacgttgc cagcgcacta cgcagggtgt
    20041 tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc gcttccagag
    20101 tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc aacgttaagg
    20161 catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg tccgaagcac
    20221 gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt ccggcttgta
    20281 gccagtcgcg gatttgcgca gcacatacct gtgccatggt actttgataa tcgccaacgc
    20341 cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca cctttaaata
    20401 caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt atttcgcgaa
    20461 acatgaacgc atcatcagtc tggctgaaaa gcttattcac gctgttcacc attcctggtg
    20521 cggaacgcca gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc gccttcatat
    20581 aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg ccaattagca
    20641 acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac tgctgggggt
    20701 cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc gccgctgcca
    20761 acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg tcatcaaaac
    20821 ccaattcgcc acggcggcgt ttttcacgcg ctactgtatc gcggatctca gccaatgcgc
    20881 gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg atcgcctcaa
    20941 acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag aaacgctggg
    21001 agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct gcccaggcgc
    21061 tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga tcaataccag
    21121 aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt tttaccgtat
    21181 caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg ggcggcggtg
    21241 ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc aacgcctgcg
    21301 gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg tagcagtggc
    21361 gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca atcagctgct
    21421 gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg caaaagccgt
    21481 gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac cactgcgcgg
    21541 cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg tcggtggttt
    21601 cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg cgcaattctg
    21661 ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc gggcggggaa
    21721 aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca atcgtaaagg
    21781 ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc aagcgcaaag
    21841 gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat tgtttgctgc
    21901 aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc ggctttcccg
    21961 ttttggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac caccgcctga
    22021 tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac gattttatca
    22081 cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga tgcttcttcg
    22141 ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat ttgcgcaaac
    22201 tcttctggct tcatcgcccg caattttgcc tctgcggttg ggaaaaacgc cttgtaacgc
    22261 tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat gccccactga
    22321 cgccccacgc tcattggaaa cgcaaacacg gcatagccca actgttcttc ggtacgcaac
    22381 tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc tgagctgctg
    22441 tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt gctgttaccg
    22501 gctttttcaa agatgacgga ttgtttttta tcgaccacga catctttgtt acgacaccat
    22561 tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt tgcctgggcc
    22621 tcagtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa ggcgtcgcga
    22681 taggccagca cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc atctcgcgag
    22741 aagtatggta cttgcgagag catctgcgcg ggcataatcg cctgctcaaa cgctttaccc
    22801 ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag ctgatcttcc
    22861 gtagcggtat agctaaagta gccctcgagc aatgcctgga acagctgcgg caggcgctgg
    22921 gtgtaaccat tggcattaac cataaggccg ttgttagcat tggtggaaaa acttatgcct
    22981 ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata atcattgagc
    23041 gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa aatcaggctg
    23101 acatcggctt tgggctcgct ggcaaaataa cggcttggcg catacaccac gcgcagattc
    23161 gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat cagcgagaaa
    23221 tcgtcaggaa tataagggtt aagctctggc aaagagagca caatgtcggc ggcttttttc
    23281 tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc atcgacaaag
    23341 taagccgttt tgttgtgcgg ctctttcggg ctgatatacc aaatacgcgc attctgcggc
    23401 gtcatcatcg caagacgttc ttttactgct ttagcatcgt accgatcagc aatattgact
    23461 gcatccaacg tatgctcaac aggaacgcga atcatggtat ctgccagcca ttcgacgtaa
    23521 tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt cgccagttca
    23581 tcgaagtatt gtttatcgat ccctttttca cgtaacagat tgaggtagct aaaaatggcc
    23641 gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga gatcgctaat
    23701 acaccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc aactaatccc
    23761 tgcttttgca gccagtcaga aagtgtacct gggctgcgat taccaatcag ataggtaatc
    23821 aattcatccg ttttactacg gaacttcgct gagttgttat cgatgcgaaa ctcaacgcgc
    23881 agaactttac gcggcagtgc cgggacgtaa tgaatgataa tgcccttttg cgcgtcggtg
    23941 actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg accaaaagtg
    24001 tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac cgccttcatc
    24061 agattggcgg aatagtactt ctcgtggaaa tctttcagtg cctgctgcac cggattacca
    24121 ggtttgtcgc ttaaagtttc gaggttaccg cctgaaaact ttgaaccggg gtgtgccggg
    24181 ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg cgccatggtt
    24241 aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag caagggttct
    24301 gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc aacttccaga
    24361 tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg cattttgaga
    24421 tattcggcca gactgtcagc ctgtgggtac tttttcgacc ccatcagact catatgttca
    24481 aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac gggcaccacc
    24541 agcgccgata gcgatttaac tgcctgcgga tcagaaacca gcaagaccac cataccgtta
    24601 tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat ggtttcctga
    24661 atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac taacaacaat
    24721 aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact ttattttctg
    24781 gccagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg ctgcacgagt
    24841 cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag taagcggata
    24901 gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg gtaacaggaa
    24961 acgttgcgac tgttcaacga tggcccccat tgtctctggt gttaattgcc gccagagcct
    25021 ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt aagcctgaag
    25081 gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat cgttttgcgc
    25141 gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca atggcgcgga
    25201 catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct gttcggctgc
    25261 aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt caccattacc
    25321 accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg ccacacttaa
    25381 taaagaggga cgccagcgca acaagccatc cggctgcacc tgcggcaacc agccagttat
    25441 ctgcacaccg ttgcaagcga gatcaatttc catactctgc cccggctggc gacaggcaat
    25501 gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa tttcaccaaa
    25561 agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt cggcatcatc
    25621 ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa gtccttccag
    25681 aataaatggc tcggtgtcgg ggatttcgct gtcttcagta cggaagttca cctgcaaacg
    25741 catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt ccagcggcac
    25801 ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt taccagcctg
    25861 gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct ggtagttttg
    25921 cggatcaaat ggcatccggg tatggaggca agtaagatgc gcttttaccc ttgcctcgct
    25981 ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga tgtagtcgat
    26041 cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg aacgcccgat
    26101 atagctgata tagatttttt gctgcgcgga aattaacgct tccaggaaca gatagcggtc
    26161 atcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt caaagcccaa
    26221 tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa ccactttgaa
    26281 cggaattgaa cgcattggca tcagagtaca aatgttaacc ggcccggcga gaaaacgctg
    26341 gctgatacgt tcttgatcca ggcgctgtgc cagttcatca cgcaatagtg acagcggcac
    26401 cgcatcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt gttgttcgat
    26461 cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga gcatatcgcg
    26521 acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac gccagatgtt
    26581 tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc cgctcgattc
    26641 atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt agcccaacaa
    26701 catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt ccagctcgcg
    26761 aacgttgtcg tcatctatcc cccaacgaat gccggactcg ttgacccact ggcgtaaata
    26821 acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat ccagcagcgc
    26881 cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga taaacgcttc
    26941 cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca ggtaacgatc
    27001 cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga tatcagccac
    27061 catcacgatg atgtcgcgcg gagtaagtgt cgggtcttcc tccagcatcg ccagcaggcg
    27121 atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg tgatactgct
    27181 atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga tgttcacacc
    27241 agcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca gcaggttatc
    27301 tggcgtgaca tcgacaaagg cgtccagctc ctgactgctc tccagatcgg aaaggagata
    27361 gatatagtcg cgccccaact tgccccaaga ggccagtagc gggttgccga catcctgttc
    27421 accgtcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg gcaattcgcg
    27481 atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag caggatcttt
    27541 aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt caatatgttt
    27601 acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac cgcatataaa
    27661 gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt cgataaagcg
    27721 ctgatagaga ttggcgcggt gccagcgcgg ttgtccgagt tcatgggtat attccaccag
    27781 cgccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa ccaaatgtcc
    27841 tgtttcccat tgtgccagcc actccgggcg atagaccaga tactggtcaa acaggtccgc
    27901 cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca gataatgccg
    27961 cagcagggta aagtcttcgc gatccagcaa ttgcggcagc agagtcatca gtttccagct
    28021 catgctctgt ttgttaaagg cgctctcttt ggggatctcc ggtaacaccc ggacgaacat
    28081 atcccagata aagctcgcag gcagcggaaa atcaatgttt gccgcaatac caaacttttg
    28141 cgacagggtc atttgcagcc actgtgccat accggtactt tgcaccagaa tcatctctgg
    28201 ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg cttccagcac
    28261 gtccagacga ttggaatggt agacccttaa catagcggct cctgactact gacgattcgg
    28321 gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg taacgctgat
    28381 gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttag taggtggcga
    28441 aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt actggtttct
    28501 gctggcaaga ctgttcatta atgttcgctg gaaacccgat aacgcagtga caatcatcac
    28561 catcaacacc atcgccaaca ttacctccgg caggctaaag ccttgttgat tcttcaggga
    28621 agctgacata acgccccctc tttcagagga caaaaatcga tccagccgcg tggcgaaaat
    28681 acgatattcc catcgatgac ttcccccatt cgccataatg aaacgtcttc ataaccggct
    28741 attagcaagg ttttattatc cgcaagtctt cgcaggcacg ctcgagcacc cgttgcggca
    28801 tattgcgaac actgaacagc ttgctgtacc tgccaggcat gcattttccc ccactcaagc
    28861 gccgactgca caatggcctg acggcgtaat gactggcttt ccatagtcac gcgagaggca
    28921 aaactgcgat cctgctggct cattccttgt aacagcaagc tgcccaaaac cagcagcatc
    28981 aggaccagtg ccagcgatga aacacccttt tcgcggttca caggttgaat cctgtcacgc
    29041 tatagctggc attcaccacg gtttgcggtt cagacttact ggcagcacgc atattaaccg
    29101 tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg tcgataatga
    29161 tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc gcaccgcgta
    29221 gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcggactct tttaccggtt
    29281 cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag tcaccctgtc
    29341 cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc ctctggagat
    29401 gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc ttacgggtgc
    29461 tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc tgcccccagc aacaatacgc
    29521 tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct tttacaggca
    29581 tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa ccaccagcca
    29641 ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat tgcgcaggcc
    29701 aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc gtggcacaaa
    29761 gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca caaggcacca
    29821 taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt tggcatcttc
    29881 acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccaaagcc gttgcgactg
    29941 ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccagaa tcagcatcgc
    30001 gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag tatggagcga
    30061 ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg actatttacg
    30121 ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac ggagggtaaa
    30181 aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag atagccaccg
    30241 gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg aaacggtagt
    30301 cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc ggttcgcggc
    30361 ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtgtcg ccccccgtcc
    30421 agacaaaatc gcccacttcc agatcacact gctgcgccat catatgcacc agtaacgcgt
    30481 agctggcaat gttaaacggc aggccgagga aaacgtcaca ggagcgctga taaagctggc
    30541 aagagagttt gccgtctgcc acatagaact ggaagaacgc atggcacggt gccagcgcca
    30601 ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa tccgggtcgt
    30661 ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc ggcgttggcc
    30721 aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg gcccattcgt
    30781 cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc tgcagaaacc
    30841 acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc agcgggaatc
    30901 catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg gttccggtac
    30961 ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa tactgtttca
    31021 tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc atcatgatca
    31081 cacccgcgac aatcatcggg atggaaagaa tttgtcccat gctgatgtac tgcacccagg
    31141 caccagtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga aacgcgccgt
    31201 aaccaatcag gaacaaacct gagacagctc ccattgggcg cggtttacga atatacaggt
    31261 tgaggataat aaacagcact acaccttcca gcaacagctc gtaaagctgt gatgggtggc
    31321 gcggcagcac accgtaagta tcgaaaatgg attgccactg cgggttggtt tgcagcagca
    31381 aaatatcttc tgtacgggag ccagggaaca gcatggcaaa cgggaagttc gggtcaacgc
    31441 ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga ccaaacggaa
    31501 tgagtggtgc gataaaatca gagacctgga agaaggaacg tttagtacgg cgggcgaaga
    31561 taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg ccgtcccaga
    31621 cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac agaacataac
    31681 caatacgtcc accgaggaag acgccgagga agcccgcata gagtaagttt tcaacttcat
    31741 ttttggtcca gccactgccc ggacgattcg cccgtcgtgt tgccagccac attgcaaaaa
    31801 tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct attgagaaaa
    31861 tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac cacaagttct
    31921 tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc aggcgctcca
    31981 aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt aaaagatatg
    32041 tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga cgctccataa
    32101 aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta cgctgcgcaa
    32161 gattttctgc gtcggcataa tcaatgcgcc gcagcaggta ttttacccgc gctacagaac
    32221 gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg cacatgggat
    32281 cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct tcccgggcga
    32341 tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc gccacccggg
    32401 tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca acagagatga
    32461 aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact tccagcatga
    32521 tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca cgtccggcac
    32581 gttcaatcag gcggcgtgct tcgtcaactt catcgaggct tgtgaccatc ggcaacagaa
    32641 tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg atcaagaaga
    32701 tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga ttctcttcgc
    32761 taatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc aaggtgacgg
    32821 gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct tcttccgacg
    32881 ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg ccatcaatac
    32941 ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg acttttatac
    33001 gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga cggctaagct
    33061 caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc ggatcgacca
    33121 gcaattcacc gcgataacca tcgacgatca gcgtccgacg atgcagcacc gaaggctgaa
    33181 tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca gcatgggagt
    33241 tggctgcgcc atcgcgcacg acaacaccga ctaagcgatc ctggggcaac tcagcaagcg
    33301 ttgtcgctga cagttcatct gccaccagaa tgaaacgttc cggccaggcg ttcggccctt
    33361 gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa tcgccagccc
    33421 gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt tcaatgaccg
    33481 tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat tcgcgacgca
    33541 gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt tctttttgtg
    33601 caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct tccagcgccc
    33661 cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac acctgttcca
    33721 ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt gctgccggta
    33781 atgcgcggat tcgcgtctgg cgatattgcc caaacagggc agtcaactgc gactgagaaa
    33841 gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt tcgtcatact
    33901 ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt tgaataattg
    33961 gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat ttgaagctgg
    34021 ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc aggccgacga
    34081 tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc ccccgggtcg
    34141 ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag acctcggtat
    34201 ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg cgtggtgcgc
    34261 tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg acttaacctc
    34321 ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc agtgacatca
    34381 ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat gacaccacct
    34441 gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt gtactgctgg
    34501 tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat ttttgttttt
    34561 ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat ttgtagcgca
    34621 accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct acttcttcaa
    34681 acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg ccttgcggaa
    34741 attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg cgattacaaa
    34801 tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta cctcaaaata
    34861 aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca ctctctgcag
    34921 ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt ataaacagag
    34981 gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg aagaactacg
    35041 gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca ttttaaaata
    35101 ttcattatta gtcaatgcat tacatgtaat ttgagtaatg aaaaagagta attcgtgacc
    35161 caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta tttatccaca
    35221 gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcatc gcacgtcaag
    35281 gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc tgtggataac
    35341 atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt gcagtgttgc
    35401 gcaactcgat taccggcagc ctaaaaagca atataaatca gggaattgaa caacacatgt
    35461 ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt aatcaaggta
    35521 tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag aacagttgtt
    35581 agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac ttgccgggct
    35641 tgttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac tggagatctg
    35701 gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gccgctctgg gcgtggaact
    35761 taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg tttgtgttgc
    35821 cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc acaagctcaa
    35881 acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc agcgtcgcgt
    35941 aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac gcgaagactg
    36001 ggaagaatta atggatatga ttgttctcgg tcaggttgag cggatcaccg ctcgtcacgg
    36061 ggaatattta cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg aagccattgg
    36121 tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga agaatttcac
    36181 cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac ctgccgcttt
    36241 ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt atttgcatgg
    36301 ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga gatcgttctt
    36361 gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac agcacaacgt
    36421 gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc gctgctggca
    36481 tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag tcaggaaatt
    36541 tctgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa agccagcaag
    36601 gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt ttcatcattc
    36661 ctcggcgcta tcgtgcagat tatgctgctg gatattatct ttagcctcga ctcggtgatt
    36721 acggctgtgg gtctgtcaga tcacctgttt attatgatgg cagccgtggt gattgccgta
    36781 ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca tccttcggta
    36841 aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct ggaaagtttc
    36901 gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat tgcggttgaa
    36961 agcctcaact tgattcgcaa caaaaagaat ccgctctgat actccgtacg ctctcctgcg
    37021 ggagggcgta ctcactcctt ttaccctcac agtttaagat tttcctgctt tcaagatata
    37081 taacgtcggt ttataaacag actattatca taggtaggcc aaatgaggat aaccggatga
    37141 aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc gggtgttcca
    37201 gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga aaacctgaaa
    37261 ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg atgcaaatta
    37321 accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca tccggctggc
    37381 cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt ccttatgtcg
    37441 tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt cgctggaagt
    37501 cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag ccgacgccca
    37561 cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg ccgaaatgta
    37621 cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg gcaactggct
    37681 ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg gaccgtcgcg
    37741 caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata tccgcgaagc
    37801 gctgcatgac agcctcaagc gcctacagac tgattacctc gatctttatc aggtgcactg
    37861 gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg attctgcgcc
    37921 tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg cgggaaaaat
    37981 tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc tgcatctggc
    38041 ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc tgttaaaccg
    38101 cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac tgctggccta
    38161 ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa aacccgctgg
    38221 cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa cgcaaaaagc
    38281 cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc agatggcgct
    38341 cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa ccacgatgga
    38401 tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg tattagctga
    38461 aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac gccggttaaa
    38521 accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat tgccagcgca
    38581 aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctatcat taccgccagc
    38641 gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg tactgcaatc
    38701 gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt gagcggaacg
    38761 acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg cagcagctcg
    38821 tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg catacagcgt
    38881 gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac gataccaatc
    38941 gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag cgccaccggt
    39001 acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa actggtgccc
    39061 accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt caggaaactg
    39121 cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag tttgggaatg
    39181 tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag ggcgacgagg
    39241 acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc cgctatggta
    39301 gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa ttcgccgaga
    39361 ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt atagccgaga
    39421 aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc gttggcaaac
    39481 atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg cgcaaaaaga
    39541 atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg atagaactgc
    39601 gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc agagagaaac
    39661 tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt gtgcactgac
    39721 tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa agtcaggttt
    39781 gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta cagcaagctc
    39841 cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt cgttatctgt
    39901 ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag tggcatggac
    39961 tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca ccatttcgcc
    40021 tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct gctcgtcaaa
    40081 acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa cattctcggc
    40141 ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca ttatgttcgg
    40201 ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca ggcgcgcatc
    40261 cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta cgttgataga
    40321 aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc caaatttatc
    40381 ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca catagcgtag
    40441 acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag tcgaggtgcc
    40501 gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatacggt aatgcagcgg
    40561 gcttggataa agaaacactt ctgcacctgt gagcagtggc gtaaacaggc ctaccgtcag
    40621 cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg tgaagtcggc
    40681 aatcgtttta atctgctcga cgttcgccag aatgctttta tggctatgga cgacgccttt
    40741 cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg gctgctgttt
    40801 tacctgtgcc agacgtggca tcagcagatg cgcgaagatc catactttgt cggcagtggt
    40861 gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt gctccggcag
    40921 atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt tgatttcagc
    40981 cgccgtaata gcactggtca gccctttcac cccggcagtg tagttcatca ttgcgggaat
    41041 gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat tgggcagcat
    41101 taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa caaacagcgt
    41161 tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga cacatttctt
    41221 cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt cacgcgggcg
    41281 caccgccagt cgcgcttcca tcattatttg atgcagcatt tcgccagcga ttttgcgacg
    41341 gtcacgggca cgcggcgcat ccggcatctc cacctgcgtt ggtggcaaaa tatgcagagt
    41401 aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt gcgtaagttc
    41461 cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga caaaacccgc
    41521 gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag ggaaaatcac
    41581 cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag gttgcgtcgg
    41641 gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact gttggcttat
    41701 tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca aaatgccatc
    41761 aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg cctgggtgtc
    41821 acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc taaaaagcat
    41881 tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca cgagataaag
    41941 aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc aggttggtgc
    42001 aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct ctgagatctt
    42061 gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat gaagggaaat
    42121 gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca aaaaaaaacc
    42181 tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac ttcacctatc
    42241 aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc ggtcaatcgc
    42301 aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc ttacttattt
    42361 gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac gggcgttttc
    42421 cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc gaccataaag
    42481 gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat taataattca
    42541 cctaaagcca gtgaagcttc ccggctggct gtgcatagtg caatggagtc tcttagctat
    42601 cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt cggtctgatc
    42661 gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga acaggtggct
    42721 tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca aaaagagcgt
    42781 caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca tgccaaaatg
    42841 atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt gctgatcaac
    42901 cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta cggtgcctgg
    42961 ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct gtgctctaac
    43021 cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct tgctgaaagt
    43081 ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag cggcggcgaa
    43141 caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc ctgttataac
    43201 gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga tgtaccgggt
    43261 gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg tccgcgcctg
    43321 accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact ggctttggcg
    43381 ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac gctggtacgt
    43441 cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag cgactaaccg
    43501 cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc gaatcaaccg
    43561 cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt agttggatga
    43621 cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg ccttcacttc
    43681 cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat cgcccgattc
    43741 acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag aacgaccatc
    43801 agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca tcagatcgtt
    43861 gaacccggca tcaaccagca caaagtggcg gctacccatt tgtttgacgc tccgcacctg
    43921 agtaattaac acgccagact gcgctaccag aaagcgaccc ggttcaattt ccagtttcac
    43981 agggtggccc aaatggcgag cgatttgctc acgcgcggca ttccacagac cataataatg
    44041 ttcggtatca accgcctctt caccctgttg ataaggaata gaaagcccac cgcccgcaga
    44101 aatagcctgt aaatcctgac cgaattcgat gacctgacgt accatagcac cacacacctg
    44161 ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga ccagctgcaa
    44221 atgattacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga taccgtgctt
    44281 gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc ccgggttaac
    44341 gcgcagccat acccgatgcc ctggcgaaac ctggcccagt tggtcgagca tatcaacaga
    44401 acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct gatcgataac
    44461 atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg ccgccaacgc
    44521 acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac gcattaatcg
    44581 caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa actgtttcag
    44641 cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc agccaaattc
    44701 agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga acagtgaatg
    44761 tggcataaca aactccagat gagtgctttt ttatgattac gccacatcat aaaaagaata
    44821 aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg attgcgagag
    44881 agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg taatgaccgc
    44941 cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg tcagccgcga
    45001 actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac gtggacgatt
    45061 acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct ggtacggact
    45121 ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag aactgtctat
    45181 tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac cctttctggc
    45241 acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac ttgaagagtg
    45301 gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc ctgcgggaac
    45361 agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg gtcatccgct
    45421 ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca tcagcctttc
    45481 ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc aggttaaacg
    45541 acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac gggcgggggt
    45601 aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg gtttagtggt
    45661 acggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc tgcaccgccc
    45721 gtcatcagcg ctggttcagg cgtttagtga gcatttacaa gcggggttac cgaaactggt
    45781 cacttctctt gacgcgattt tgtcgtcagc tacgacagca taaaagcgac agcatcctcg
    45841 gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg aaccaataaa
    45901 ccaatttctg tgcagccaaa aatgacaccc tgcgccccct gttctgcaag gcgagcaatc
    45961 acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag ttcttcaaaa
    46021 ataatctgat taatcttcgc ccgttcatcc gcttccggaa taagacaatt gatggaaaat
    46081 tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt acccagcagc
    46141 gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc aatgtgtaag
    46201 aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt attggtacat
    46261 aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc cagaatgtcc
    46321 ccggttttat cccattcccc gcgacgctgg cactcttcta tttcatgaaa atcgacgcta
    46381 tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc ttcatttatc
    46441 agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc aattgttttc
    46501 attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga gacaaacgcc
    46561 tcaacggcca ggtgcccaat ctctattaac gaaaaaaggg ccggatgtat agcacatccg
    46621 gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt ttgcgttcga
    46681 tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg cccacaaacg
    46741 caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgctg tcgagcagcg
    46801 tcaggaaggt cgcgccgata atcatattcg acacccagtt cgtggtggtc gaacaggtga
    46861 tgccaaaatc gcggcatttc agcggctgaa tttcagagca caggatccac accactggcg
    46921 cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag agccaggaca
    46981 agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc accagagtgc
    47041 ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct ttatctaccg
    47101 taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga gtcgcaatca
    47161 tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca tagtacatga
    47221 tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga aacacagcac
    47281 gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac ttcaggcttt
    47341 cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc atacgcaaca
    47401 cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta tttggcagga
    47461 agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac attgcgcgcc
    47521 agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg ccgagtgtga
    47581 ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc atttcagaaa
    47641 gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc acacgagcag
    47701 cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt acaaacagga
    47761 tggcccccgc catcaggctg tatttacgcc ccaggcggaa cgacagccaa ccattaaaca
    47821 gcgcaccaat tgctgcaccg agcatcatgc tactaaccac ccattcctgc aaacgactgg
    47881 tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg atatcaagac
    47941 caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata cgccgcgtat
    48001 cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt ttttcctgcc
    48061 agcagagagt aagacatagt gaaaaaatac gtgaacaact cacacaggtg tcaggtcgga
    48121 aacagcataa atatggatta aattgctacg acatgtcgtt atgtgatgga tattccaatt
    48181 ttcaaattaa gttgaattat tgatattatt attaaccacc taattttaca gcagataaaa
    48241 ttcataaagt tcattaattg ataattgata tggattattt cataaccatg atatggatta
    48301 tgatgatcta cagatataaa aaaccctgcc atgcggcagg gtcataaaag taagaagaat
    48361 gaattaacgc gccagccaac cgccatccac ggcaatggta taaccattca cataatctga
    48421 agcgctggag gcaaggaaca ctaccggccc catcaggtca ctcggcagtc cccaacggcc
    48481 agctggaatg cggtcgagga tttccgcgct acgttgttca tctgcccgta gttgttgagt
    48541 attgttggtc gccatgtaac ccggggctat cgcattaaca ttaatgttgt gtttagccca
    48601 ttcgttcgcc atcaatcgcg tcacacccat cacgccgctt tttgatgcgg tataagaagg
    48661 cacacggatc ccgccctgga aggagagcat tgacgcgata ttgataatct tgccgccatt
    48721 gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc tcttgatatt
    48781 caggttcatg acatcgtccc agtctttttc gctgaactcg agagcgtctt cgcggcgaat
    48841 caatccggcg ttattcacca ggatatcaat atgaccaaac tccgctaccg cgcgatccag
    48901 cagtgctgga ataccatcaa tctttcgcag atcggcggtc aggcttaaaa aacgacgccc
    48961 cagcgccgtg acctgcttga tggtttcagt cggttcaacg atgttaatgc caacaatgtc
    49021 acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat cacagccagt
    49081 gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa taccttattc
    49141 gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc atcaaagacc
    49201 tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac gccggaatga
    49261 atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatgtg acgcgtttct
    49321 tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa atagaaataa
    49381 acttccatcc ggcgctcgtg agtgtgacac ggcatggtgt tccacaagtt acctggagcc
    49441 agctccgtca gccccatact caattggcag gtttccagca catccgggac aaaatatttg
    49501 ttaattgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga tacttcatcc
    49561 ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta ataaaacttt
    49621 gccggagtgg cggtatcaat actggcaaag acaacttctt ttgcaccttt acccacatac
    49681 agggcgtcgc ggtgaccgat ttcatagcat tggccatcga cagtaatcgt accggcaccg
    49741 ccaatattga taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc gagttgttta
    49801 ccaacttccc cgccaacgga aaccgtttta gttatcggca taatgccgcc aacaataatt
    49861 cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctc ttcaaccaaa
    49921 aattcattgc gcagcccttg ggtatccagc gtttttgcgt gcgcactgtg gatgctctgt
    49981 cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa cgatagtgaa
    50041 acactgtttc gttttctcta taaagggatg atatgcccat gggctagggt tttcaattaa
    50101 aaataaaaca atgtttcatt tttaagttaa ggattaaaaa aagtgcagtg tcgatcacga
    50161 atatgtcatg agaatggaaa aatgagggag cccagaaaat gtatgtcgac ttgccagcca
    50221 tcattattca acagattaat gacggcagat atttatcggt aaggaggaaa tgcagagcta
    50281 ctcgtcacgt tcaatggtca atgccacgcc ctggcccccg ccgatacaaa gcgttgccag
    50341 ccctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaaccagga ttcggcaacc
    50401 ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca ttgacattga cccgacgctc
    50461 atcccactca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt cattagcctc
    50521 gataagatcg acatcagcca actgccagcc tacacgctcc aggcaacggc gggtcgcata
    50581 caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg cgcggatgcg
    50641 ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca ttaccgcagc
    50701 tgcgccatcg tttatggatg atgcattccc cgcggtcaca gaaccgagac tatcaaatga
    50761 aggatttaaa cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt catcggtatc
    50821 aacaaccaac gtctgaccgt tactttgggt cattaccggg acgatctcat ctttaaatcg
    50881 tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag catcctgcaa
    50941 ttgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa tatgataatc
    51001 attgaaggca tcccataacc catcatgcac aagactgtca accagctggc tattgccaag
    51061 ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg cggctcatgt tttcctggcc
    51121 accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca gatgcagtgc
    51181 tttaagtccg gaaccgcaga cgtcattaat cgtgattgca gaaacgctat taggcagacc
    51241 acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca acacctgacc
    51301 aagaattact tcatccaccg catatgcagg aacgccggta cgttctatta acgctttcac
    51361 gaccagacta ccaagttcca cggcggaatg acccgctaac gcaccgcgaa agcagccgat
    51421 aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc accaattaac
    51481 agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa agtgattttt
    51541 atcacaaagg aaatatgcct gagcagtagt cagagtcata actggcacgt aaggtttgca
    51601 accactaacc caccaataga ggggtagata gggcgttaat ctcccatact taacctggtt
    51661 tatggtaaat tgccctccat tttgtttaat ttgtagatga tacgttcaga taatgtctaa
    51721 tatttggtct aaagaagaaa ctctgtggag tttcgcgctc tacggcacag ccgttggtgc
    51781 aggcacgctc ttccttccta ttcagttagg ttcggcaggg gctgtggtcc tgtttattac
    51841 tgctctggtc gcctggcctt taacatattg gccacataaa gccttatgcc agttcatcct
    51901 ctcatcgaaa acatcagcag gtgaagggat aacgggcgca gtaacacact actatggcaa
    51961 gaagattggt aatctgatta ccacgctgta cttcatcgcc ttttttgtcg tcgtgttgat
    52021 atatgcagtg gcaattacca actcacttac ggaacagctg gcaaagcata tggttattga
    52081 tcttcgcatc cgtatgttgg tgagtctggg tgttgtatta atcctgaatc tcatttttct
    52141 gatgggacgc catgcaacca ttcgggtaat gggttttttg gtattcccat tgattgccta
    52201 tttcttattt ctttccattt accttgtcgg tagttggcaa cctgatctat taacgaccca
    52261 ggtagagttc aatccgaata cccttcacca gatatggata tcgattcccg tgatggtttt
    52321 cgcctttagc catacgccca ttatttctac gtttgccata gacagacgtg aaaaatatgg
    52381 cgaacacgct atggataaat gcaaaaaaat tatgaaagtc gcttatctca tcatctgcat
    52441 aagtgtactg ttctttgtct ttagctgcct gctttctatt ccaccttcgt atattgaagc
    52501 ggctaaagaa gaaggggtca ccattttatc ggcgctttct atgctgccga acgccccagc
    52561 atggctgtca atttccggga ttattgtcgc agtagttgcg atgtcgaaat cattcctggg
    52621 tacgtacttt ggcgttattg aaggtgccac ggaggtcgtc aaaacaacac tacagcaggt
    52681 tggtgtaaag aaaagtcgtg catttaaccg cgcactatca attatgttgg tatcgctgat
    52741 caccttcatt gtttgttgca ttaacccgaa cgcgatttcg atgatttacg cgatcagcgg
    52801 cccgctcatt gccatgatac ttttcatcat gcctacgttg tcaacgtatc tgatcccggc
    52861 gcttaaaccc tggcgttcca tcggaaatct gattacgctg atcgtgggta tcctgtgcgt
    52921 atcggtaatg ttctttagct gatatccatt gtgaatggtt caaaattgct acaattttga
    52981 accattccag ttttcataat gttcattcgt tattattccg cactatcact tccctcatca
    53041 tatcctagta ttatcagtta gttaaacaca caaagagatc tgaacatctt aaataagact
    53101 atttaagatg cataacttag attcgcaaga tatcttctgg tcattcaaaa acaatttgcg
    53161 atatagatat tc
//
LOCUS       DAZKIV010000014        50560 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZKIV010000014 DAZKIV010000000
VERSION     DAZKIV010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.014"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            120..2654
                     /gene="mrcB"
                     /locus_tag="ACHOT6_000878"
     CDS             120..2654
                     /gene="mrcB"
                     /locus_tag="ACHOT6_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFP9946139.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRSKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            2874..5117
                     /gene="fhuA"
                     /locus_tag="ACHOT6_000879"
     CDS             2874..5117
                     /gene="fhuA"
                     /locus_tag="ACHOT6_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFP9946140.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            5168..5965
                     /gene="fhuC"
                     /locus_tag="ACHOT6_000880"
     CDS             5168..5965
                     /gene="fhuC"
                     /locus_tag="ACHOT6_000880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFP9946141.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSCCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            5965..6855
                     /gene="fhuD"
                     /locus_tag="ACHOT6_000881"
     CDS             5965..6855
                     /gene="fhuD"
                     /locus_tag="ACHOT6_000881"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFP9946142.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAIPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            6852..8834
                     /gene="fhuB"
                     /locus_tag="ACHOT6_000882"
     CDS             6852..8834
                     /gene="fhuB"
                     /locus_tag="ACHOT6_000882"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFP9946143.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(8869..10149)
                     /gene="hemL"
                     /locus_tag="ACHOT6_000883"
     CDS             complement(8869..10149)
                     /gene="hemL"
                     /locus_tag="ACHOT6_000883"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFP9946144.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLLE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            10315..10362
                     /gene="yadX"
                     /locus_tag="ACHOT6_000884"
     CDS             10315..10362
                     /gene="yadX"
                     /locus_tag="ACHOT6_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFP9946145.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            10374..11795
                     /gene="clcA"
                     /locus_tag="ACHOT6_000885"
     CDS             10374..11795
                     /gene="clcA"
                     /locus_tag="ACHOT6_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFP9946146.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            11819..11884
                     /gene="yadW"
                     /locus_tag="ACHOT6_000886"
     CDS             11819..11884
                     /gene="yadW"
                     /locus_tag="ACHOT6_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFP9946147.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            11877..12221
                     /gene="erpA"
                     /locus_tag="ACHOT6_000887"
     CDS             11877..12221
                     /gene="erpA"
                     /locus_tag="ACHOT6_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFP9946148.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(12268..12891)
                     /gene="yadS"
                     /locus_tag="ACHOT6_000888"
     CDS             complement(12268..12891)
                     /gene="yadS"
                     /locus_tag="ACHOT6_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFP9946149.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(12929..13729)
                     /gene="btuF"
                     /locus_tag="ACHOT6_000889"
     CDS             complement(12929..13729)
                     /gene="btuF"
                     /locus_tag="ACHOT6_000889"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFP9946150.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEPVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(13722..14420)
                     /gene="mtnN"
                     /locus_tag="ACHOT6_000890"
     CDS             complement(13722..14420)
                     /gene="mtnN"
                     /locus_tag="ACHOT6_000890"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFP9946151.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            14504..16021
                     /gene="dgt"
                     /locus_tag="ACHOT6_000891"
     CDS             14504..16021
                     /gene="dgt"
                     /locus_tag="ACHOT6_000891"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFP9946152.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFI
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFAELVEKERVKRFPIEMRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            16151..17575
                     /gene="degP"
                     /locus_tag="ACHOT6_000892"
     CDS             16151..17575
                     /gene="degP"
                     /locus_tag="ACHOT6_000892"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFP9946153.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQGGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            17730..18887
                     /gene="cdaR"
                     /locus_tag="ACHOT6_000893"
     CDS             17730..18887
                     /gene="cdaR"
                     /locus_tag="ACHOT6_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFP9946154.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(18976..19362)
                     /gene="yaeH"
                     /locus_tag="ACHOT6_000894"
     CDS             complement(18976..19362)
                     /gene="yaeH"
                     /locus_tag="ACHOT6_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFP9946155.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(19677..20501)
                     /gene="dapD"
                     /locus_tag="ACHOT6_000895"
     CDS             complement(19677..20501)
                     /gene="dapD"
                     /locus_tag="ACHOT6_000895"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFP9946156.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(20532..23204)
                     /gene="glnD"
                     /locus_tag="ACHOT6_000896"
     CDS             complement(20532..23204)
                     /gene="glnD"
                     /locus_tag="ACHOT6_000896"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFP9946157.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAVLFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(23266..24060)
                     /gene="map"
                     /locus_tag="ACHOT6_000897"
     CDS             complement(23266..24060)
                     /gene="map"
                     /locus_tag="ACHOT6_000897"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFP9946158.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            24428..25153
                     /gene="rpsB"
                     /locus_tag="ACHOT6_000898"
     CDS             24428..25153
                     /gene="rpsB"
                     /locus_tag="ACHOT6_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFP9946159.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            25411..26262
                     /gene="tsf"
                     /locus_tag="ACHOT6_000899"
     CDS             25411..26262
                     /gene="tsf"
                     /locus_tag="ACHOT6_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFP9946160.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            26409..27134
                     /gene="pyrH"
                     /locus_tag="ACHOT6_000900"
     CDS             26409..27134
                     /gene="pyrH"
                     /locus_tag="ACHOT6_000900"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFP9946161.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            27426..27983
                     /gene="frr"
                     /locus_tag="ACHOT6_000901"
     CDS             27426..27983
                     /gene="frr"
                     /locus_tag="ACHOT6_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFP9946162.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            28075..29271
                     /gene="ispC"
                     /locus_tag="ACHOT6_000902"
     CDS             28075..29271
                     /gene="ispC"
                     /locus_tag="ACHOT6_000902"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFP9946163.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPENFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMEAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVLHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            29460..30218
                     /gene="ispU"
                     /locus_tag="ACHOT6_000903"
                     /gene_synonym="uppS"
     CDS             29460..30218
                     /gene="ispU"
                     /locus_tag="ACHOT6_000903"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFP9946164.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            30231..31088
                     /gene="cdsA"
                     /locus_tag="ACHOT6_000904"
     CDS             30231..31088
                     /gene="cdsA"
                     /locus_tag="ACHOT6_000904"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9946165.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            31100..32452
                     /gene="rseP"
                     /locus_tag="ACHOT6_000905"
     CDS             31100..32452
                     /gene="rseP"
                     /locus_tag="ACHOT6_000905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFP9946166.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            32482..34914
                     /gene="bamA"
                     /locus_tag="ACHOT6_000906"
     CDS             32482..34914
                     /gene="bamA"
                     /locus_tag="ACHOT6_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFP9946167.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            35036..35521
                     /gene="skp"
                     /locus_tag="ACHOT6_000907"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             35036..35521
                     /gene="skp"
                     /locus_tag="ACHOT6_000907"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFP9946168.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            35525..36550
                     /gene="lpxD"
                     /locus_tag="ACHOT6_000908"
     CDS             35525..36550
                     /gene="lpxD"
                     /locus_tag="ACHOT6_000908"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9946169.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            36655..37110
                     /gene="fabZ"
                     /locus_tag="ACHOT6_000909"
     CDS             36655..37110
                     /gene="fabZ"
                     /locus_tag="ACHOT6_000909"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFP9946170.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            37114..37902
                     /gene="lpxA"
                     /locus_tag="ACHOT6_000910"
     CDS             37114..37902
                     /gene="lpxA"
                     /locus_tag="ACHOT6_000910"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFP9946171.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            37902..39050
                     /gene="lpxB"
                     /locus_tag="ACHOT6_000911"
     CDS             37902..39050
                     /gene="lpxB"
                     /locus_tag="ACHOT6_000911"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFP9946172.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLADRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            39047..39643
                     /gene="rnhB"
                     /locus_tag="ACHOT6_000912"
     CDS             39047..39643
                     /gene="rnhB"
                     /locus_tag="ACHOT6_000912"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFP9946173.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRAPGLAS"
     gene            39680..43162
                     /gene="dnaE"
                     /locus_tag="ACHOT6_000913"
     CDS             39680..43162
                     /gene="dnaE"
                     /locus_tag="ACHOT6_000913"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFP9946174.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            43175..44134
                     /gene="accA"
                     /locus_tag="ACHOT6_000914"
     CDS             43175..44134
                     /gene="accA"
                     /locus_tag="ACHOT6_000914"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFP9946175.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            44233..46374
                     /gene="ldcC"
                     /locus_tag="ACHOT6_000915"
     CDS             44233..46374
                     /gene="ldcC"
                     /locus_tag="ACHOT6_000915"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFP9946176.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            46431..46820
                     /gene="yaeR"
                     /locus_tag="ACHOT6_000916"
     CDS             46431..46820
                     /gene="yaeR"
                     /locus_tag="ACHOT6_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9946177.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            46885..48183
                     /gene="tilS"
                     /locus_tag="ACHOT6_000917"
     CDS             46885..48183
                     /gene="tilS"
                     /locus_tag="ACHOT6_000917"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFP9946178.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENLGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGALQIAPMLAMSDARRAAIIRRWLAGKNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGNIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(48232..48492)
                     /gene="rof"
                     /locus_tag="ACHOT6_000918"
     CDS             complement(48232..48492)
                     /gene="rof"
                     /locus_tag="ACHOT6_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFP9946179.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(48479..48679)
                     /gene="yaeP"
                     /locus_tag="ACHOT6_000919"
     CDS             complement(48479..48679)
                     /gene="yaeP"
                     /locus_tag="ACHOT6_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFP9946180.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            48845..49390
                     /gene="yaeQ"
                     /locus_tag="ACHOT6_000920"
     CDS             48845..49390
                     /gene="yaeQ"
                     /locus_tag="ACHOT6_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFP9946181.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            49387..49809
                     /gene="arfB"
                     /locus_tag="ACHOT6_000921"
     CDS             49387..49809
                     /gene="arfB"
                     /locus_tag="ACHOT6_000921"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFP9946182.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            49823..50533
                     /gene="nlpE"
                     /locus_tag="ACHOT6_000922"
                     /gene_synonym="cutF"
     CDS             49823..50533
                     /gene="nlpE"
                     /locus_tag="ACHOT6_000922"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFP9946183.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPLTPMTLRGMYFYMADVA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        11926 a        12440 c        14070 g        12124 t
ORIGIN      
        1 cctctccctc aagggagagg ggaccgatcg agcacaaatt ttgagagata tcttcttctg
       61 tcttgtaaca gaagaacaga aaatcgggct tttgcgcctg aatattgcgg agaaaaagca
      121 tggccgggaa tgaccgcgag ccaattggac gcaaagggaa accgacgcgt ccggtcaaac
      181 aaaaggtaag ccgtcgtcgt tacgaagatg acgatgatta cgacgattat gatgactatg
      241 aggatgaaga accgatgccg cgcaaaggta agggcaaagg caaagggcgt aagcctcgta
      301 gcaaacgcgg ctggctatgg ctactgctaa aactggctat cgtttttgcc gtgctgatcg
      361 ccatttacgg cgtttatctc gatcaaaaaa ttcgtagccg tattgatggc aaggtctggc
      421 aactgcctgc ggcagtttat ggccgaatgg tcaatcttga gccagatatg accatcagca
      481 agaacgagat ggtgaagcta ctggaggcga ctcagtatcg tcaggtgtcg aaaatgaccc
      541 gtcctggcga atttaccgtg caggccaaca gcattgagat gatccgccgt ccgtttgatt
      601 tcccggacag taaagaaggg caggtgcgtg cgcgtctgac ctttgatggt gatcatctgg
      661 cgacgatcgt caatatggag aacaaccgtc agttcggttt cttccgtctt gatccgcgcc
      721 tgatcaccat gatctcctcg ccaaacggtg agcagcgttt gtttgtgccg cgcagtggtt
      781 tccctgattt gctggtggat actttgctgg cgacagaaga ccgtcatttt tacgagcatg
      841 acggcatcag cctctactcc atcggacgtg cggtgctggc aaacctgacc gccggacgca
      901 cggtgcaggg agcgagtacg ctgacgcaac agctggtgaa aaacctgttc ctctccagtg
      961 agcgttctta ctggcgtaaa gcgaacgaag cttacatggc gctgatcatg gacgcgcgtt
     1021 acagcaaaga ccgtattctt gagctgtata tgaacgaggt gtatctcggt cagagcggcg
     1081 acaacgaaat ccgcggcttc ccgctggcga gcttgtatta ctttggtcgc ccggtagaag
     1141 agctaagcct cgaccagcag gcgctgttag tcggcatggt gaaaggggcg tccatctaca
     1201 acccgtggcg taacccgaaa ctggcgctgg agcgacgtaa tctggtgctg cgtctgctgc
     1261 aacagcaaca gattattgat caagaactct atgacatgtt gagtgcacgt ccgctggggg
     1321 ttcagccgcg cggtggggta atctctccac agccagcctt tatgcaactg gtgcgtcagg
     1381 agctgcaggc aaaactgggc gataaggtaa aagatctctc cggcgtgaag atcttcacta
     1441 cctttgactc ggtggcccag gacgcggcag aaaaagccgc cgtggaaggc attccggcac
     1501 tgaagaaaca gcgtaagctg agcgatctgg aaaccgcgat tgtggtcgtc gaccgcttta
     1561 gtggtgaagt tcgtgcgatg gtcggaggtt ctgagccgca gtttgcgggc tacaaccgtg
     1621 cgatgcaggc gcgtcgttcg attggttccc ttgcaaaacc agcgacttat ctgactgcct
     1681 taagccagcc gaaaatctat cgcctgaaca cgtggattgc ggatgcgcca atcgcgctgc
     1741 gtcagccgaa tggccaggtc tggtcaccgc agaacgatga tcgtcgttat agcgaaagtg
     1801 gcagagtgat gctggtggat gcgttgaccc gttcgatgaa cgtgccgacg gtaaatctgg
     1861 ggatggcact ggggctgcct gcggttacgg agacctggat caaactgggc gtgccgaaag
     1921 atcagctgca tccggttccg gcgatgctgc tgggggcgtt gaacttaacg ccaatcgaag
     1981 tggcgcaggc attccagacc atcgccagcg gcggtaaccg tgcaccgctt tctgcgctgc
     2041 gttcggtaat cgcggaagat ggcaaagtgc tgtatcagag cttcccgcag gcggaacgcg
     2101 ctgttccggc gcaggcggcg tatctgacac tatggaccat gcagcaggtg gtacaacgcg
     2161 gtacgggtcg tcagcttggg gcgaaatacc cgaacctgca tctggcaggg aaaacaggga
     2221 ctactaacaa taacgtagat acctggtttg cgggcattga cggcagcacg gtgaccatca
     2281 cctgggtagg ccgtgataac aaccagccga ccaaactgta tggagccagc ggggcaatgt
     2341 cgatttatca gcgttatctg gctaaccaga cgccaacacc gctgaatctt gttccgccag
     2401 aagatattgc agatatgggc gtggactacg acggcaactt tgtttgcagc ggtggcatgc
     2461 gtgtcttgcc ggtctggacc agcgatccgc aatcgctgtg ccagcagagt gagatgcagc
     2521 agcagccgtc aggcaatccg tttgatcagt cttctcagcc gcagcaacag ccgcaacagc
     2581 aacctgctca gcaagagcag aaagacagcg acggtgtagc cggttggatt aaagatatgt
     2641 ttggtagtaa ttaacgtcta agcgtgaaat accggatggc gagttgccat ccggtaaaat
     2701 aacatcccac ccaaagtatt aaccctttct tttcatctgg ttgtttatta acccttcagg
     2761 aacgctcaga ttgcgtaccg cttgcgaacc cgccagcgtt tcgaatatta tcttatcttt
     2821 ataataatca ttctcgttta cgttatcatt cactttacat cagagatata ccaatggcgc
     2881 gttccaaaac tgctcagcca aaacactcac tgcgtaaaat cgcagttgta gtagccacag
     2941 cggttagcgg catgtctgtt tatgcacagg cagcggttga accgaaagaa gacactatca
     3001 ccgttaccgc tgcacctgcg ccgcaagaaa gcgcatgggg gcctgctgca actattgcgg
     3061 cgcgacagtc agctaccggc actaaaaccg atacgccgat tcaaaaagtg ccacagtcta
     3121 tttctgttgt gaccgccgaa gagatggcgc tgcatcagcc gaagtcggta aaagaagcgc
     3181 ttagctacac gccgggtgtc tctgttggta cgcgtggcgc atccaacacc tatgaccacc
     3241 tgatcattcg cggttttgcg gcagaaggcc aaagccagaa taactatctg aatggcctga
     3301 agttgcaggg caacttctat aacgatgcgg tcattgatcc gtatatgctg gaacgcgctg
     3361 aaattatgcg tggcccggtt tccgtgcttt acggtaaaag cagtcctggc ggtctgttga
     3421 atatggtcag caagcgtccg accaccgaac cgctcaaaga agttcagttt aaagccggta
     3481 ctgacagcct gttccagact ggttttgact ttagcgatgc gctggatgat gacggcgttt
     3541 actcttatcg cctgaccggt cttgcgcgtt ctgccaatgc ccagcagaaa gggtcagaag
     3601 agcagcgtta tgctattgca ccggcgttca cctggcgtcc ggatgataaa accaatttca
     3661 ccttcctttc ttacttccag aacgagccgg aaaccggtta ttacggctgg ttgccgaaag
     3721 agggaaccgt tgagccgctg ccgaacggta agcgtctgcc gacagacttt aatgaagggg
     3781 cgaagaacaa cacctattct cgtaatgaga agatggtcgg ctacagcttc gatcacgaat
     3841 ttaacgacac ctttactgtg cgtcagaacc tgcgctttgc tgaaaacaaa acctcgcaaa
     3901 acagcgttta tggttacggc gtctgctccg atccggcgaa tgcttacagc aaacagtgtg
     3961 cggcattagc gccagcggat aaaggccatt atctggcacg taaatacgtc gttgatgatg
     4021 agaagctgca aaacttctcc gttgataccc agttgcagag caagtttgcc actggcgata
     4081 tcgaccacac cctgctgacc ggtgtcgact ttatgcgtat gcgtaatgac atcaacgcct
     4141 ggtttggtta cgacgactct gtgccactgc tcaatctgta caatccggtg aataccgatt
     4201 tcgacttcaa tgccaaagat ccggcaaact ccggccctta ccgcattctg aataaacaga
     4261 aacaaacggg cgtttatgtt caggatcagg cgcagtggga taaagtgctg gtcaccctgg
     4321 gcggtcgtta tgactgggca gatcaagaat ctcttaaccg cgttgccggg acgaccgata
     4381 aacgtgatga caaacagttt acctggcgtg gtggtgttaa ctacctgttt gataatggcg
     4441 taacacctta ctttagctat agcgaatcgt ttgaaccttc ttcgcaagtt gggaaggatg
     4501 gtaatatttt cgcaccgtct aaaggtaagc agtatgaagt cggcgtgaaa tatgtaccgg
     4561 aagatcgtcc gattgtagtt actggtgccg tgtataatct cactaaaacc aacaacctga
     4621 tggcggaccc tgagggttcc ttcttctcgg ttgaaggtgg cgagatccgc gcacgtggcg
     4681 tagaaatcga agcgaaagcg gcgctgtcgg cgagtgttaa cgtagtcggt tcttatactt
     4741 acaccgatgc ggaatacacc accgatacta cctataaagg caatacgcct gcacaggtgc
     4801 caaaacacat ggcttcactg tgggctgact ataccttctt tgacggtccg ctttcaggtc
     4861 tgacgctggg caccggtggt cgttatactg gctccagcta tggtgatccg gctaactcct
     4921 ttaaagtggg aagttatacg gtcgtggatg cgttagtgcg ttatgatctg gcgcgagtcg
     4981 gcatggctgg ctccaacgtg gcgctgcatg ttaacaacct gttcgatcgt gaatacgtcg
     5041 ccagctgctt taacacttat ggctgcttct ggggcgcaga acgtcaggtc gttgcaaccg
     5101 caaccttccg tttctaattt ctcttttggg gcacggattt ccgtgcccat ttcacaagtt
     5161 ggctgttatg caggaataca cgaatcattc cgataccact tttgcactgc gtaatatctc
     5221 ctttcgtgtg cccgggcgca cgcttttgca tccgctgtcg ttaacctttc ctgccgggaa
     5281 agtgaccggt ctgattggtc acaacggttc tggtaaatcc actctgctca aaatgcttgg
     5341 ccgtcatcag cctccgtcag aaggggagat tcttcttgat gcccagccgc tggaaagctg
     5401 gagcagcaaa gcgtttgccc gcaaagtggc ttatttgccg cagcagcttc ctccggcaga
     5461 agggatgacc gtgcgtgaac tggtggcgat tggtcgttac ccgtggcatg gcgcgttggg
     5521 gcgctttggg gcggcagatc gcgaaaaagt cgaggaagct atctcgctgg ttggcttaaa
     5581 accgctggcg catcggctgg tcgatagtct ctctggcggc gaacgtcaac gggcgtggat
     5641 cgccatgctg gtggcgcagg atagctgttg tctgttgctc gacgaaccga cttcggcgct
     5701 ggatatcgcc caccaggttg atgtgctggc gctggtgcac cgtttaagtc aggagcgtgg
     5761 cctgacggtc attgccgtgt tgcacgatat caatatggcg gcacgctact gtgattatct
     5821 ggtcgccctg cgcggtggtg aaatgattgc tcagggaacg cctgcggaaa ttatgcgcgg
     5881 cgaaactctc gaaatgattt atggcatccc gatgggtatt ttgccgcatc cggcgggtgc
     5941 tgcacctgtg agttttgttt attgatgagc ggcttacctc ttatttcgcg ccgtcgactg
     6001 ttaacggcga tggcgctttc tccgttgtta tggcagatga ataccgccca cgcggcggct
     6061 attgacccca atcgtattgt ggcgctggag tggttgccgg tggaattact gctggcgctc
     6121 ggcatcgtgc cttacggcgt ggcggatacc atcaactatc gcctgtgggt cagcgaacca
     6181 ccattgccgg actcagtgat tgacgtcggt ttgcgtacag aacctaacct tgaactgctg
     6241 accgaaatga aaccgtcgtt tatggtctgg tcggcaggat atggcccttc accagaaatg
     6301 ctggcacgta ttgcgccggg tcgcggattt aacttcagtg acggcaaaca gccgttggcg
     6361 atggcgcgta aatcgctgac ggaaatggca gatttactta acctgcaaag cgcagcagaa
     6421 acgcatttag cgcactatga agactttatc cgcagcatga aaccccgctt tgtgaagcgt
     6481 ggtgcacgcc cgttattgct gacgacgctt atcgatccgc gccatatgct ggtcttcggt
     6541 ccaaacagct tgttccagga aattctcgat gagtacggca tcccaaatgc ctggcaaggg
     6601 gaaaccaact tctggggcag taccgccgtc agtatcgatc gtctggcggc gtataaagac
     6661 gttgatgtgc tctgttttga tcacgacaac agcaaagaca tggatgcgct aatggcaacc
     6721 ccgctgtggc aggccatacc gtttgtccgc gccggacgct ttcagcgcgt acctgcagtc
     6781 tggttttatg gtgcgacgct ctcggcaatg cactttgtgc gcgttctgga taacgccatc
     6841 ggaggtaaag cgtgagtaaa cgaattgcgc tttttccggc gttattgctg gcgctgttag
     6901 tgattgtcgc tacggcgctc acttggatga acttctcgca ggcgctgccg cgtagccagt
     6961 gggcgcaggc tgcctggtcg ccggatattg acgtcatcga gcagatgatt tttcactaca
     7021 gcttgttgcc gcgtctggcg atttcgctgc tggtgggcgc gggcctgggg ctggtgggcg
     7081 tgctgtttca gcaagtgctg cgtaacccgc tggcggaacc gacgacgttg ggcgttgcaa
     7141 caggcgcgca actggggatt accgtcacta cgctctgggc gatcccaggt gctatggcga
     7201 gccagtttgc tgcgctggca ggggcttgcg ttgttggctt aattgtcttt ggcgtcgcgt
     7261 gggggaaacg gctttcgccg gtaacgctta ttctcgcggg gctggtagtg agcctttatt
     7321 gtggggcaat caatcagtta ctggttatct tccatcatga ccaactgcaa agcatgttcc
     7381 tgtggagcac cggaacgctg acgcaaaccg actggggcgg cgttgagcgt ttatggccgc
     7441 agttgctggg cggcgtgatg ctgacgttat tgctacttcg cccgttaacc ctgatggggc
     7501 ttgatgatgg cgtggcgcgc aatctcgggc tggccttatc gcttgcgcgt ctggctgcgc
     7561 tgtcgctggc gatagtcatc agtgcgctgc tggtgaacgc agtggggatt atcggcttta
     7621 tcgggttgtt cgcgccactg ctggcaaaaa tgctgggggc gcggcgtttg ttgccacggt
     7681 tgatgctggc gtcgctgatt ggtgcgttaa ttctgtggct ttctgatcaa atcatcctct
     7741 ggctgactcg cgtgtggatg gaagtttcca ccggttcggt cactgcgttg atcggtgcgc
     7801 cgctgctact gtggctgctg ccgcgtttac gcagcattag cgcgccggat atgaaggtta
     7861 acgatcgtgt cgcgactgaa cgccaacatg tgctggcgtt tgccctcgcg ggcggcgtgc
     7921 tgttgttgat ggctgtggtg gtggcgctgt cgtttggtcg tgatgcgcac ggctggacgt
     7981 gggcgagcgg ggcgttgctc gaggatttaa tgccctggcg ctggccgcga attatggcgg
     8041 cgctgtttgc gggcgtcatg ctggcggtag cgggctgtat tattcagcga ctgaccggca
     8101 acccgatggc aagcccggaa gtgctgggga ttagctccgg cgcggcgttt ggtgtggtgt
     8161 taatgctgtt tctggtaccg ggtaatgcct ttggctggct gttacccgca ggcagtctcg
     8221 gggcggcggt gacgctgttg atcattatga tcgccgccgg acgaggtgga ttttccccac
     8281 accgcatgtt actggcgggg atggcgttaa gtaccgcgtt caccatgctt ttgatgatgt
     8341 tgcaggcaag tggtgacccg cgaatggcgc aagtgctgac ctggatttcc ggttcgacct
     8401 acaacgcgac cgatgcgcag gtctggcgca ccggaattgt gatggtgatt ttgctggcga
     8461 ttaccccgct gtgccgccgc tggctgacca ttttaccgct gggtggtgat accgcccgag
     8521 ccgtaggaat ggcgctaacg ccgacgcgaa ttgcgctgct gctgttagcg gcttgcctga
     8581 cggcgaccgc gacgatgact attggaccgt tgagttttgt tggtttaatg gcaccgcata
     8641 ttgcgcggat gatgggcttt cgacggacga tgccgcacat cgtaatttcg gcgctggtgg
     8701 gtggtttact gctggtgttc gctgactggt gtgggcggat ggtgctgttt ccattccaga
     8761 tcccggcggg gctgctgtca acctttatcg gcgcgccata ttttatctat ttgttgagaa
     8821 agcagagccg ttaattgaat gcctcgcccg gtgggcgagg caaaatcatc acaacttcgc
     8881 aaacacccga cgtgcagcat cgatggtgtt attgatatct tccatgctgt gcgccacgga
     8941 cataaagccc gcttcaaacg ctgacggtgc caggtaaaca ccttcatcca gcatcatatg
     9001 gaagaaacgc ttaaagcgtt ccacgtcaca ggccatcaca tcctgatagc acgtcacgga
     9061 ctcggcgtcg gtaaagaaaa taccgaacat gccgccaacg tggttaacga ccagcggaat
     9121 tccggcttct tctgccgctt ccagcagacc ttctgccaga cgtgatgtca gctcatccaa
     9181 cgtttcgtga acgcccggct gcgcgacttc attcagacag gcgaaaccgg ctgccatcgc
     9241 aattgggtta ccggaaagcg tacccgcctg atagaccgga cccgtcgggg ccagcgcatc
     9301 cattacatca cgacgaccac cgaatgcgcc taccggcatt ccaccgccga tgatttttcc
     9361 gaggcaggtt aaatctggca ctacgccgta ataatcctgt gcaccagcca gtgccacgcg
     9421 gaagccagtc attacttcat cgatgatcag caatgcgcca aattcgtcgc acagcgcgcg
     9481 cagacctggc aggaactcta gcagcggcgg aacacagttc atattgcctg ccaccggctc
     9541 aacgataata caggcaatct cttgcgggta ttgctcaaac gcggcgcgta cagaagccag
     9601 atcgttataa gtacaggtta aggtatgttt ggcgaaatct gccggaacac ccggcgagtt
     9661 tggctggcct aacgtgagtg cgccagaacc ggctttcacc agcaggcagt cagcgtgacc
     9721 gtggtaacaa ccttcaaatt taatgatttt gtcgcgaccg gtaaaaccac gggccaggcg
     9781 gatggcgctc atggtcgctt cagtgccgga gttcaccatg cgcaccatat ccatggtcgg
     9841 gaccagttcg gtcaccagtt gcgccatttt cacttccatt tcggttggtg caccaaagct
     9901 taaaccacgc tcggcggctt caatcacggc attgcggatt gccggatggt tatggcccag
     9961 caccatcggc ccccaggaac cgacataatc gatataggct ttgccatcaa catcgtacag
    10021 ataagcgccg tccgcttttt cgataaacag tggagtgccg cccacgccag taaaggcgcg
    10081 aacaggggag ttcacaccgc cagggatcag ctcgcgcgct gcgctgtaaa gattttcaga
    10141 cttactcatg gagggttcct gattcgtaga aaaagtgaat ggctgctatt ctatgttatt
    10201 cataacaagt taaatactcg tcaaacatca ggctgcttgt actggtgcaa tcctgatttc
    10261 ttagagtata aaagttttgt gcatttgaaa cattacgctt tgcaaaggat tttcatggaa
    10321 cgtgcgagta aaatgccgtc atcttatttg tatgaccaat aagtgatcat tggatgaaaa
    10381 ctgatactcc ctctttagaa acaccgcagg ccgcgcgcct gcgacgcaga caactgattc
    10441 gccaacttct tgagcgcgat aaaaccccgt tagccatttt gtttatggcg gcagtcgtcg
    10501 gcacgcttgt cgggctggca gcggttgctt ttgacaaagg tgtcgcctgg ttgcagaacc
    10561 aacgtatggg ggcgctggta catactgctg ataattatcc gctactgtta accgtcgctt
    10621 ttctctgttc ggcggtgctg gcgatgttcg gctacttttt ggtgcgcaaa tacgcgcccg
    10681 aagcaggggg atcggggatc ccggaaattg aaggggcgct ggaagatcaa cgtcctgttc
    10741 gctggtggcg tgtattgccg gtgaaattct ttggcgggct ggggacactc ggcggaggca
    10801 tggtgctagg gcgcgaagga ccaaccgtgc agatcggcgg caatatcggt cgcatggtgc
    10861 ttgatatttt ccgcctgaaa ggtgatgaag ctcgccatac gctgctggca accggtgctg
    10921 ctgcggggct ggctgcggcc tttaacgcgc cgctggcggg tattttgttt attatcgaag
    10981 agatgcgtcc gcagtttcgc tatacgttaa tttcgattaa agcggtattt attggtgtca
    11041 ttatgtcgac cattatgtac cggattttta atcatgaagt tgcgttgatt gacgtcggta
    11101 aactttctga tgcgccgctt aatacgctgt ggctttatct gatcctcggt attatttttg
    11161 gcattttcgg ccctattttt aataaatggg tgctggggat gcaggatttg ctgcaccgtg
    11221 tgcacggcgg caatattacc aaatgggtac tgatgggtgg tgcgattggc ggtctgtgtg
    11281 gattgctggg gtttgtggca ccagcaacgt cgggcggcgg ttttaacctg attcctatcg
    11341 ctaccgcggg gaatttcagc atgggaatgc tggtgtttat cttcgtcgct cgggtcatta
    11401 ccaccttact ctgcttctct tccggcgcgc cgggcggtat ttttgccccg atgctggcgc
    11461 tgggtactgt gctgggaacc gctttcggaa tggttgccgt tgagctgttt ccgcaatatc
    11521 acctggaggc ggggacgttt gctattgccg gaatgggggc attactggcg gcatctattc
    11581 gcgcgccgtt aacggggatc attctggttc tggagatgac cgataactac cagctcattt
    11641 tgccaatgat tattaccggt cttggcgcaa cactattagc gcaatttacc ggcgggaaac
    11701 cgctatactc ggcgattctt gcgcgcacgc tggcaaaaca ggaagctgag caactggcgc
    11761 gaagcaaggc cgcatcagcc agcgagaata cttgaacgaa ataccagggt attagataat
    11821 ggcgattatt attgggttag aatttgccca attgccgatg tcgtttggag caaaatatga
    11881 gtgatgacgt agcactgccg ctggagttta ccgacgcagc agccaacaaa gttaaaagcc
    11941 tgatcgctga cgaagataac ccgaatctga aattacgcgt gtatatcacc ggtggcggtt
    12001 gcagcggctt ccagtatggt ttcacctttg atgatcaggt gaacgaaggc gatatgacca
    12061 tcgaaaaaca gggcgttggc ctggtggttg atccgatgag cctgcaatat ctggtcggcg
    12121 gttccgttga ttataccgaa ggtctggaag gttctcgttt catcgtgacc aacccgaacg
    12181 cgaaaagcac ctgcggttgc ggttcttcct ttagtatcta atctgttgtt catgtgccgg
    12241 ataaactatc cggcacatct tctctcttca acgcccattc tcatccagcg caaacgtcgg
    12301 tagcttaaga tgccaacgaa tagccgccag ccgaatcaat agcgtcacga ccatgcccat
    12361 catactggct gtttccagtg gtacggaaaa tgtgtaataa gccgtagcgt ggacaatacc
    12421 gccgataata caggcagttg cgtagatttc tgtacgtaaa atcatgggga tttcgcgggc
    12481 cagtacatca cgaatgatcc cgccgccaac gccagtaatg acgcccatac aaaccgcgat
    12541 taacggaccg gcttccgcat taaaggcttt attcacgcca atgccgacaa acaccgccag
    12601 accaacggcg tccagcaccg gcaacatcca ttttggtaag cgtcttggct ggcgcaccag
    12661 cacgatggtc agcatgctgg tgaccattgc aacgaccaga tcggtgggat ctttcaccca
    12721 aaataccggg ccgtgatcca gcgccatgtc gcgaattgtt ccgccgccta ctgcggtaac
    12781 cacgcccagt accagaacac caaaagggtc catacgcaat tttccggcta acaaaacgcc
    12841 ggagatggca aataccgctg tgccgactat atccagccaa tagacgagca ttgttcaatc
    12901 cccactgagc acctgaaaag gtcaggcact aatctacctg tgaaagcgca ttacagagct
    12961 gttgtgcagc gaggataata cgtgggcttg cacgttcaaa ccagtcactc gtgagaggaa
    13021 taacgggaat tttgagctgt tcaccccagt attgtttgat tttaggaatt tggtccggtc
    13081 cgcctgtaat gacaatcgcc tgtggcgagc gtgctaacac ctgttcgcgg ctaacttgcg
    13141 gccagggaac ccggctgtct ttaaagatgt tttctccgcc acaaacttcg agtacctggt
    13201 tctgaatcga ctcttttcca ctggtaaatg gcggattaat gccgaattgc agaaaaacac
    13261 gttttttagg tttatcagca tattgcgctt tcaattgcgc gtactgatcc agcagggatt
    13321 gcgcggcttg ttcggccttg tctggttgcg gactccaggg ggccagttga cgtaacgcat
    13381 tggcaatttg ttcaatgctt gtcgcatcga cccacatcac ttttattccc agcgaagcca
    13441 gctggtcaac ctgccgctcg gcattacctc cacgccaggc aatcaccaga tcgggtttca
    13501 gcgcgacaat gcgttccaga ttcatcccct gccaggtgga aaccggctca atcttttgcg
    13561 cttgtggagg atagtcggaa tagctgctga ccccaaccgg cgtgatcccg gcggcaaagg
    13621 caagttcagt gttggcggga gaaagcgtga tgacgcgcgg cgcggcgttg agccacagtg
    13681 gcgcaagaaa agacagggcg accagcgccc tgaacagtga cttagccatg tgcaagtttc
    13741 tgcaccagtg actcaaccat caggctggac tgtttagcgg caacagccag gaactcatcg
    13801 aagctaagat gagactgttg atcggccacg tcggagatgg cgcgtacgac aacaaacggg
    13861 acgttgaaat tgtggcagac atgggcgatt gccgtcgctt ccatctctac agcaatggcc
    13921 tgtgggaagt tgtggcggat tttcgccaga ccaacagaac cgttgatgaa agcgtcgccg
    13981 ctaacaatca ggccacgtac agcgttaaga ttcagttcgg caatgcaggc ctcagcggca
    14041 gcgatcagtt tatcgtcagc tttaaagcct gccggacagc ctggtaactg accgtattca
    14101 taaccaaatg ccgtgacatc cgcgtcgtga taacgtgctt cgtccgagac aacgatatcg
    14161 cccactttca acgttggtgc caggccaccg gcagaaccgg tgttaataat cacatctggc
    14221 ttgcagtgtt ccaacagcaa agtggcaccc agcgccgcag cgactttacc gatgcccgat
    14281 ttcagaagcg caacctcggt tccattcagt tggccggtat agatttcgca accgccgaga
    14341 ctgatagttt gacggttttc gattttgtca cgcagcagcg taacttcttc ttccattgca
    14401 ccaatgatgc cgattttcat agatttactc gcgataagcc cgatttgaag gcatagttta
    14461 ccatgcgctt acggggaagc gtatttctca cgcgggagag gacatggcac agattgattt
    14521 ccgaaaaaaa ataaactggc atcgtcgtta ccgttcaccg cagggcgtta aaaccgaaca
    14581 tgagatcctg cggatcttcg agagcgatcg cgggcgtatc atcaactctc cggcaattcg
    14641 tcgtctgcaa caaaagaccc aggtttttcc actggagcgc aatgccgccg tgcgcacgcg
    14701 tcttacccac tcgatggaag tccagcaggt ggggcgctac atcgccaaag aaattttaag
    14761 ccgtctgaaa gagcttaaat tactggaagc atacggcctg gatgaactga ccggcccctt
    14821 tgaaagcatt gttgagatgt catgcctgat gcacgatatc ggcaatccgc cgtttggtca
    14881 ttttggcgaa gcggcgataa atgattggtt tcgccagcgt ttgcacccgg aagatgccga
    14941 aagccagcct ctgactgacg atcgctgcag cgtggcggca ctacgtttac gggacgggga
    15001 agaaccgctt aacgagctgc ggcgcaagat tcgtcaggac ttatgtcatt ttgaggggaa
    15061 tgcacaaggc attcgtctgg tgcatacatt gatgaggatg aatctcacct gggcacaggt
    15121 tggcggtatt ttaaaatata cccgtccggc gtggtggcgt ggcgaaacgc ctgagacaca
    15181 tcactattta atgaaaaagc cgggttatta tctttctgaa gaagcctata ttgcccggtt
    15241 gcgtaaagaa cttaatttgg cgctttacag tcgttttcca ttaacgtgga ttatggaagc
    15301 tgccgacgac atctcctatt gtgtggcaga ccttgaagat gcggtagaga aaagaatatt
    15361 taccgttgag cagctttatc atcatttgca cgaggcgtgg ggtcagcatg agaaaggttc
    15421 gctcttttcg ctagtggttg aaaatgcctg ggaaaaatca cgctcaaata gtttaagccg
    15481 cagtacggaa gatcagtttt ttatgtattt acgggtaaac accctaaata aactggtacc
    15541 atacgcggca caacgattta ttgataatct gcctgcgatt ttcgccggaa cgtttaatca
    15601 tgcattattg gaagatgcca gcgaatgcag cgatcttctt aaactatata aaaatgtcgc
    15661 tgtaaaacat gtgtttatcc atccagatgt cgagcagctt gaattgcagg gctatcgggt
    15721 cattagcgga ttattagaga tttatcgtcc tttattaagc ctgtcgttat ccgactttgc
    15781 tgaactggta gaaaaagaac gggtgaaacg tttccctatt gaaatgcgct tattccacaa
    15841 actctcgacg cgccatcggc tggcctatgt cgaggctgtc agtaaattac cgtcagattc
    15901 tcctgagttt ccgctatggg aatattatta ccgttgccgc ctgctgcagg attatatcag
    15961 cggtatgacc gacctctatg cgtgggatga ataccgacgt ctgatggccg tagaacaata
    16021 accaggcttt tgtaaagacg aacaataaat ttttaccttt tgcagaaact ttagttcgga
    16081 acttcgcgct ataaaacgaa tctgaagaac acagcaattt tgcgttatct gttaatcgag
    16141 actgaaatac atgaaaaaaa ccacattagc actgagtgca ctggctctga gtttaggttt
    16201 ggcgttatct ccgctctctg caacggcggc tgagacttct tcagcaacga cagcccagca
    16261 gatgccaagc cttgcaccga tgctcgaaaa ggtgatgcct tcagtggtca gcattaacgt
    16321 agaaggtagc acaaccgtta atacgccgcg tatgccgcgt aatttccagc agttcttcgg
    16381 tgatgattct ccgttctgcc agggaggttc accgttccag agctctccgt tctgccaggg
    16441 gggccagggc ggtaatggcg gcggccagca acagaaattc atggcgctgg gttccggcgt
    16501 catcattgat gccgataaag gctatgtcgt caccaacaac cacgttgttg ataacgcgac
    16561 ggtgattaaa gtccagctga gcgatggtcg taagttcgac gcgaagatgg ttggcaaaga
    16621 tccgcgctct gatatcgcgc tgattcagat ccagaacccg aaaaacctga ccgcaattaa
    16681 gatggcggat tctgatgcac tgcgcgtggg tgattacacc gtagcgattg gtaacccgtt
    16741 tggcctggga gagacggtaa cttccgggat tgtctctgcg ctggggcgta gcggcctgaa
    16801 tgccgaaaac tacgaaaact tcatccagac cgatgcagcg atcaaccgtg gtaactccgg
    16861 tggtgcgctg gttaacctga acggcgaact gatcggtatc aacaccgcga tcctcgcacc
    16921 ggacggcggc aacatcggta tcggttttgc tatcccgagc aacatggtga aaaacctgac
    16981 ctcgcagatg gtggaatacg gccaggtgaa acgcggtgag ctgggtatta tggggactga
    17041 gctgaattcc gaactggcga aagcgatgaa agttgacgcc cagcgcggtg ctttcgtaag
    17101 ccaggttctg cctaattcct ccgctgcaaa agcgggcatt aaagcgggtg atgtgatcac
    17161 ctcactgaac ggtaagccaa tcagcagctt tgccgcactg cgtgctcagg tgggtactat
    17221 gccggtgggc agcaaactga ccttgggctt gctgcgcgac ggtaagcagg tcaacgtgaa
    17281 tctggaactg cagcagagca gccagaatca ggttgattcc agctccatct tcaacggcat
    17341 tgaaggtgct gagatgagca acaaaggcaa agatcagggc gtggtagtga acaacgtgaa
    17401 aacgggcact ccggctgcgc agatcggcct gaagaaaggt gatgtgatta ttggcgcgaa
    17461 ccagcaggca gtgaaaaaca tcgctgaact gcgtaaagtt ctcgacagca aaccgtctgt
    17521 gctggcactg aacattcagc gcggcgacag caccatctac ctgttaatgc agtaatctcc
    17581 ctcaacccct tcctgaaaac gggaaggggt tctccttaca atctgtgaac ttcaccacaa
    17641 ctccatacat cttcatcatc ctttgggcat ttgcacaatg ccgtacgtta cgtacttcct
    17701 tatgctaagc cgtgcataac ggaggactta tggctggctg gcatcttgat accaaaatgg
    17761 cgcaggatat cgtggcacgt accatgcgca tcatcgatac caatatcaac gtaatggatg
    17821 cccgcgggcg aattatcggc agcggcgatc gtgagcgtat tggtgaattg cacgaaggtg
    17881 cattgctggt actttcacag ggacgagtcg tcgatatcga tgacgcggtg gcacgccatc
    17941 tgcacggtgt gcggcagggg attaatctac cgttacggct ggaaggtgaa attgtcggcg
    18001 taattggcct gacaggtgaa ccagagaatc tgcgtaaata tggcgaactg gtctgcatga
    18061 cggctgaaat gatgctggaa cagtcgcggt tgatgcacct gttggcgcag gatagccgtt
    18121 tgcgggaaga actggtgatg aacctgattc aggctgagga gaatactccc gcacttactg
    18181 aatgggcgca acggctgggg atcgatctca atcaaccgcg agtagtggct attgttgagg
    18241 tcgacagcgg tcagcttggc gtggacagcg caatggcgga gttacaacaa ctgcaaaatg
    18301 cgctgactac gcccgagcgt aataatctgg tggcgattgt ctcgctgacc gaaatggtgg
    18361 tgttgaaacc ggcgttgaac tcttttgggc gctgggatgc agaagatcat cgtaagcgag
    18421 ttgaacaact gattacccgc atgaaagagt atggccagct gcgttttcgc gtttcgctgg
    18481 gcaactattt taccggtcct ggcagtattg cccgatccta tcgtacggcg aaaacgacga
    18541 tggtggtggg taaacagcgg atgccagaaa gtcgctgcta tttttatcag gatctgatgt
    18601 tacctgtgtt actcgacagt ttgcgtggcg actggcaggc caatgaactg gcgcgaccgc
    18661 tggcgcggct gaaagcgatg gacaataatg gcttgctgcg acgaacgctg gcggcgtggt
    18721 ttcgccacaa tgtgcaaccg ctggctacgt caaaggcgct gtttattcat cgtaatactc
    18781 tggagtatcg gcttaatcgt atatcggaac tgaccgggct tgatttgggt aattttgatg
    18841 acaggttgct gctgtatgtg gcgttgcagc tggatgaaga gcggtaggtt atgcgttaag
    18901 gtcgaggcgg cgctggctca ttcccctcag cccgaagggt agaggggaat gtccgagtta
    18961 accgtcagtt cggcattatt tacgcgttag tttttccaga tcggcttcaa tctcgctgat
    19021 cttattggtt acgactgact ccaggtgacg taagtcgtcg aggatcttac gcttaagatc
    19081 aacttcgctg cggtcacgct ggcagatttg atcaagctca tcaatgatat accgtagatt
    19141 cgggctgatt tcctggactt ctttataacc ctgacccaca ccatcagcga cgaccgtttt
    19201 acgctgacgc ggatatttaa acttaacgct cttggcgaaa aactcgcctt tgtctttctg
    19261 gaaatagatt ttcagaatat cgttgttggc ttcctgccgg aggctgtaac gatcaatttc
    19321 ttcaggattg gtaataccca gacttttcag attgtcgtac atagcgttac ctcaaaatga
    19381 gtcagtaaat tgtgcttatt ttagcatttg gcctggcccg cccggctcga ctgtgatcgc
    19441 agaaagcctt tttcttccag acttctatca tggcgtaaaa atcaaaaaat tacctgcttt
    19501 attgtggcga tattccatgg gatggattaa cacatttatc ttcgttttgc gaattacatg
    19561 acagtccgaa acaagatgaa aaagtattta aaaaaggggg gaagaagaat gagtaggacg
    19621 aattattctg agccagaaag catacgggca gcatcatgct gcccgtatat gtactcttaa
    19681 tcgatggtac gcagcagttc gttaatgccg actttgccgc gagttttcgc gtcaactttc
    19741 ttaacgataa ccgcacagta gaggctgtat ttgccatctt tcgacggcag attacctgaa
    19801 acaaccacag accccgccgg aacgcgaccg tagtggattt cgccggtttc acggtcgtaa
    19861 atacgggtgc tctgaccaat gtatacgccc atggaaatga cggaaccttc ttcgacaatc
    19921 accccttcaa ccacttcaga gcgtgcgcca atgaagcagt tatcttcaat gatggttggg
    19981 ttagcctgca gcggttccag cacgccgccg atgcccacgc caccggaaag gtggacgttt
    20041 ttaccaatct gcgcacaaga accgacggtc gcccaggtat caaccatggt gccttcatca
    20101 acatatgcgc cgatgttgac gtaagacggc atcagcacgg tgttacgggc aataaacgca
    20161 ccctgacgta ccgccgctgg tggcacaacg cggaagcctt ctttctggaa acgtgcttcg
    20221 tcgtagtcgg cgaatttcat cggcactttg tcgaagtagc ggctttctgc cccttcgatc
    20281 acctgattat cattaatacg gaaagagagc agcaccgctt ttttcaacca ctgatgcgtc
    20341 acccactgac cgtcaatttt ttccgctaca cgcagtgcac cggaatccag cagggcgatc
    20401 acctgattta ccgcttcgcg ggtaacggtg tctgcgttgg ctggcgtgat ctcggcacgg
    20461 cgttcaaaag cggtttcaat aatgttctgt aactgctgca ttgttaaact cttttcatat
    20521 cagtaaacac atcacccttt atcgtttgga ttgagggcct ctgtcaaccg ctgatgcact
    20581 tcctgctgca actcgttatt aagtgcacgc cggtcagcgg tggcaattat gaataaatct
    20641 tctactcgct cgccaatggt tgtaattcgg gcaccatgaa gcgaaattcc cagatcggca
    20701 aaaattttcc cgactctcgc cagcagtcct ggctggtcga gcgcgataag ctcaaggaac
    20761 gatttgcggt cggtatgggt cggcaaaaac gttacttcgg tttcaacagt aaaatggcgt
    20821 aatttggcgg gttggcgacg gggctgcggt ggctgccagc tactttgcgt cagtacttgc
    20881 tccagaccaa accgaataac ctcatgacga tctgcggaca gcgggctgcc atcgggttcc
    20941 agcacgataa aggtatccat cgccataccg tcgcgagtgg tgaaaatttg tgcgtcgtga
    21001 acacttaaat tgcggcggtc taattcggca cagacggcgg caaacagata ggggcggtcc
    21061 gggctccaga taaaaatctc ggtgcctcca cgcgtagcct gcgggctaag caataccagc
    21121 ggtttgctta aatcatgctg taataaatgg cgggcatgcc aggccagttg atttgggcta
    21181 tggcggacaa aatagttagc acgacagcgt gaccaaattt ggtgcagcgc ctcttcgtcg
    21241 atgttatcca tgcgcagtag tgccagtgcc tggagttggt gatggcgaac ccgttcgcgc
    21301 atatccggcg tgttttgcat tccgcgtcgt agctgctttt cggtggcaaa atagagttcg
    21361 cgcaacagac tttgcttcca gctattccac agcgtttcgt tggtggcgca aatgtcagcc
    21421 actgtcaggc ataccagata gcgcagacga ttttccgttt gaacttcttc ggcaaactgc
    21481 ttgatgactt cagggtcctg aatatcgcgg cgttgtgctg tcaccgacat cagcaagtgc
    21541 tggcgaacca gccaggcaac cagctgtgtt tcgcgtgagt tcagcccgtg gagttctgca
    21601 aaatgcacta catcctgagc accgagaatg gagtggtcgc cgccgcgtcc tttggcgata
    21661 tcgtgaaaca gcacggcgat gaaaatcagc tcagttgacg gcaggcgcgg ccagacgtcc
    21721 acacacaacg gatggcgctg gcgcgtttct tcactggcaa aactctccag tttcagcatc
    21781 acgcggatag tatgttcatc caccgtgtag gcgtggaaca gatcaaactg catctgcccg
    21841 acgatatgcg accattgcgg catatacgcg ccgagcacgc tatggcgatg cattggcaat
    21901 agcccgcgcc gcaccgctcc ggggtgacgc agaatgctca aaaacagttt tcgtgcttcc
    21961 ggaatattac acagcggttg ttgcagatgg cgacgggcat ggcgtaactg gcgcagcgtg
    22021 gtggagtaaa tgccggtgat cgcactgttg cgcaccatgg tgtagaacat acgcaagatg
    22081 gcttccggct ggcgcataaa tagtgtttca tcacgcaggt cgattagcgt accgcgtagc
    22141 tgaaactcat cgtcgattgg acgtggtttt tcgtcggcgg gaagggcgag gatggcttca
    22201 tcgaacagtt gcagcagcat ctggttgagt tcactgacgc ggcgtgtaac gcggaagtaa
    22261 tccttcatca tccgctcgac cggctcgttg ccttcaccac tgtaattcag acgctgagcg
    22321 acgctaagtt ggcgatcgaa taacaggcga ttatcgtaac ggctgacgac cagatgcagg
    22381 gcaaagcgaa tacgccacag tatatgcaga cattcgttta attccgcccg ctccgctgag
    22441 gttaagaagc caaacccaac catttcatcc agcgatgttg cgccaaaatg acggcgggcc
    22501 acccattgca gagtatggat atcgcgcaag ccgccagggc tacttttgat gtctggttca
    22561 agattgtagc tggtgccgtg gtaacgctga tggcgctggt tctgttcttc aactttcgcc
    22621 gcgtagaact tgtcggaagg ccagaatcct tcgctaaaaa tatgtttttg cagttcgagg
    22681 aagagcgcaa catcgccaat taataagcgg gattcgatta aattggtggc gacggttaaa
    22741 tccgataacc cttccagcat gcactcttca agcgtgcgca cactatgacc gacttccagc
    22801 tttacatccc agagcagcgt taacagctcg cccacttttt gcgcctgatc gtccgggagc
    22861 tttttacggc ttaaaatcag taaatcgatg tcagaaagtg gatgcagctc gccacgaccg
    22921 tagccaccga cggcgaccaa tgccaggtcg gcaatctggc tgaatcccgc ttcaatccat
    22981 aatcgttgca ggagctggtc gataaactcg gtgcgcgcct caatcaactg ttctgcagag
    23041 atcccattgt caaaggcatc acccagccaa cgctggaaag tatcgatatg ggcttttatc
    23101 ccaccgacgg ttaattcatc acggggccag acgcatggat tttgcggttg accgggcagg
    23161 gtggggagag cggtgtttgc gtactgttct ggaagggtat tcattgtgcg ccacccataa
    23221 gattaaatta tcgcattaaa aaagccggca ttcgccggct tcatcttatt cgtcgtgcga
    23281 gattatcgcc gggatggtgt catccttgcg tagcgtcaga atttcgcagc cgttatcagt
    23341 caccacaata gtatgctcat attgtgcaga caagctgcga tctttggttt ttaccgtcca
    23401 gccatctttc atggtgcgga tctctttttt acccgcgttg accattggct cgatggtgaa
    23461 cgtcatccca ggtttcagta cgacgttggt ttcacgggag tcatagtgca gcacctgcgg
    23521 ttcttcatgg aagccgcgac caataccgtg tccgcaatat tcacgaacga cggagaagcc
    23581 ttctgcttcg acaaatttct gaatcgccgc accgatttcg cgcagattaa tgcctggttt
    23641 gaccatgcgc agcgccaggt acaggctttc ttgcgtgatg cggcacagac gttcgcccat
    23701 gatggtcggc ttaccgacga taaacatttt cgaggtatcg ccgtggaaac catctttgat
    23761 tacggtgaca tcaatgttaa cgatatcgcc atctttcagc agcttcgcat cgtccgggat
    23821 accgtggcac accacttcat taatagagat gcaaacggat ttcggatagc cgtgatagcc
    23881 gaggcaggca gaaaccgcgt gttgttcatt aacaatgtaa tcattacaga tgcgatccag
    23941 ctcgccggtg ctgacgcccg gtttaacata cggttcgatc atctccagca cttcggcagc
    24001 cagtcggcca gcgacgcgca ttttttcgat atcttctggg gtcttgattg agatagccat
    24061 taattctgtc catcagcgtc ggtgataccg acaatatata tgtaagtgcc gtcaatggta
    24121 tcacacccgg gcaaattgag aatcattctg aatttcgcca aacgtgccac tgaacgtttt
    24181 ctataataga aaattcaacg tctgatgctg tacacagcgc caacaattat tggtgtccat
    24241 gacgtatttg tggtataaag cgcgccggac ttccgatcca tttcgtatac acagactgga
    24301 cggaagcgac aatctcactt tgtgtaacaa cacacacgta tcggcacata ttccggggtg
    24361 ccctttgggg tcggtaatat gggatacgtg gaggcataac cccaactttt atatagaggt
    24421 tttaatcatg gcaactgttt ccatgcgcga catgctcaag gctggtgttc acttcggtca
    24481 ccagacccgt tactggaacc cgaaaatgaa gccgttcatc ttcggtgcgc gtaacaaagt
    24541 tcacatcatc aaccttgaga aaactgtacc gatgttcaac gaagctctgg ctgaactgaa
    24601 caagattgct tctcgcaaag gtaaaatcct tttcgttggt actaaacgcg ctgcaagcga
    24661 agcggtgaaa gacgctgctc tgagctgcga ccagttcttc gtgaaccatc gctggctggg
    24721 cggtatgctg actaactgga aaaccgttcg tcagtccatc aaacgtctga aagacctgga
    24781 aactcagtct caggacggta ctttcgacaa gctgaccaag aaagaagcgc tgatgcgcac
    24841 tcgtgagctg gagaaactgg aaaacagcct gggcggtatc aaagacatgg gcggtctgcc
    24901 ggacgctctg tttgtaatcg atgctgacca cgaacacatt gctatcaaag aagcaaacaa
    24961 cctgggtatt ccggtatttg ctatcgttga taccaactct gatccggacg gtgttgactt
    25021 cgttatcccg ggtaacgacg acgcaatccg tgctgtgacc ctgtacctgg gcgctgttgc
    25081 tgcaaccgta cgtgaaggcc gttctcagga tctggcttcc caggcggaag aaagcttcgt
    25141 agaagctgag taataaggct tgataactcc cccaaaatag ttcgagttgc agaaaggcgg
    25201 caagctcgag aattcccggg agcttacatc agtaagtgac cgggatgagc gagcgaagac
    25261 aacgcacctg cggcgcgaaa tatgaagggg gagagccctt atagaccagg tagtacacgt
    25321 ttggttaggg ggcctgcata tggccccctt tttcactttt atatctgtgc ggtttaatgc
    25381 cgggcagatc acatctccga ggattttaga atggctgaaa ttaccgcatc cctggtaaaa
    25441 gagctgcgtg agcgtactgg cgcaggcatg atggattgca aaaaagcact gactgaagct
    25501 aacggcgaca tcgagctggc aatcgaaaac atgcgtaagt ccggtgctat taaagcagcg
    25561 aaaaaagcag gcaacgttgc tgctgacggc gtgatcaaaa ccaaaatcga cggcaactac
    25621 ggcatcattc tggaagttaa ctgccagact gacttcgttg caaaagacgc tggtttccag
    25681 gcgttcgcag acaaagttct ggacgcagct gttgctggca aaatcactga cgttgaagtt
    25741 ctgaaagcac agttcgaaga agaacgtgtt gcgctggtag cgaaaattgg tgagaacatc
    25801 aacattcgcc gcgttgctgc gctggaaggc gacgttctgg gttcttatca gcacggtgcg
    25861 cgtatcggcg ttctggttgc tgctaaaggc gctgacgaag agctggttaa acacatcgct
    25921 atgcacgttg ctgcaagcaa gccagaattc atcaaaccag aagacgtatc cgctgaagtg
    25981 gtagaaaaag aataccaggt acagctggat atcgcgatgc agtctggtaa gccgaaagaa
    26041 atcgcagaga aaatggttga aggccgcatg aagaaattca ccggcgaagt ttctctgacc
    26101 ggtcagccgt tcgttatgga accaagcaaa actgttggtc agctgctgaa agagcataac
    26161 gctgaagtga ctggcttcat ccgcttcgaa gtgggtgaag gcatcgagaa agttgagact
    26221 gactttgcag cagaagttgc tgcgatgtcc aagcagtctt aattatcgaa aaggagccgc
    26281 ctgagggcgg cttctttttg tgcccatctt gtaaattcag ctaacccttg tggggctgcg
    26341 ctgaaaagcg acgtacaatg tcgctagtat taattcattt caatcgttga cagtctcagg
    26401 aaagaaacat ggctaccaat gcaaaacccg tctataaacg cattctgctt aagttgagtg
    26461 gcgaagctct gcagggcact gaaggcttcg gtattgatgc aagcatactg gatcgtatgg
    26521 ctcaggaaat caaagaactg gttgaactgg gtattcaggt tggtgtggtg attggtgggg
    26581 gtaacctgtt ccgtggcgct ggtctggcga aagcgggtat gaaccgcgtt gtgggcgacc
    26641 acatggggat gctggcgacc gtaatgaacg gcctggcaat gcgtgatgca ctgcaccgcg
    26701 cctatgtgaa cgctcgtctg atgtccgcta ttccattgaa tggcgtgtgc gacagctaca
    26761 gctgggcaga agctatcagc ctgttgcgca acaaccgtgt ggtgatcctc tccgccggta
    26821 caggtaaccc gttctttacc accgactcag cagcttgcct gcgtggtatc gaaattgaag
    26881 ccgatgtggt gctgaaagca accaaagttg acggcgtgtt taccgctgat ccggcgaaag
    26941 atccaaccgc aaccatgtac gagcaactga cttacagcga agtgctggaa aaagagctga
    27001 aagtcatgga cctggcggcc ttcacgctgg ctcgtgacca taaattaccg attcgtgttt
    27061 tcaatatgaa caaaccgggt gcgctgcgcc gtgtggtaat gggtgaaaaa gaagggactt
    27121 taatcacgga ataattcccg tgatggataa ataagggtaa gattccgcgt aagtatcgcg
    27181 ggggcgttag tctggttata aggcgttatt gttgcaggca gtttggtcac ggccagcgcg
    27241 cagcaaccgg agcgtacaaa agtacgtgag gatggcgagc actgctcggg gccaaaatgg
    27301 caaataaaat agcctaataa tccagacgat tacccgtaat atgtttaatc agggctatac
    27361 ttagcacact tccactgtgt gtgactgtct ggtctgactg agacaagttt tcaaggattc
    27421 gtaacgtgat tagcgatatc agaaaagatg ctgaagtacg catggacaaa tgcgtagaag
    27481 cgttcaaaac ccaaatcagc aaaatacgca cgggtcgtgc ttctcccagc ctgctggatg
    27541 gcattgtcgt ggaatattac ggcacgccga cgccgctgcg tcagctggca agcgtaacgg
    27601 tagaagattc ccgtacactg aaaatcaacg tgtttgatcg ttcaatgtct ccggccgttg
    27661 aaaaagcgat tatggcgtcc gatcttggcc tgaacccgaa ctctgcgggt agcgacatcc
    27721 gtgttccgct gccgccgctg acggaagaac gtcgtaaaga tctgaccaaa atcgttcgtg
    27781 gtgaagcaga acaagcgcgt gttgcagtac gtaacgtgcg tcgtgacgcg aacgacaaag
    27841 tgaaagcact gttgaaagat aaagagatca gcgaagacga cgatcgccgt tctcaggacg
    27901 atgtacagaa actgactgat gctgcaatca agaaaattga agcggcgctg gcagacaaag
    27961 aagcagaact gatgcagttc tgatttcttg aacgacaaaa acgccgctca gtagatcctt
    28021 gcggatcggc tggcggcgtt ttgcttttta ttctgtctca actctggatg tttcatgaag
    28081 caactcacca ttctgggctc gaccggctcg attggttgca gcacgctgga cgtggtgcgc
    28141 cataatcccg aaaacttccg cgtagttgcg ctggtggcag gcaaaaatgt cactcgcatg
    28201 gtagaacagt gcctggaatt ctctccccgc tatgccgtaa tggacgatga agcgagtgcg
    28261 aaacttctta aaacgatgct acagcaacag ggtagccgca ccgaagtctt aagtgggcaa
    28321 caagccgctt gcgatatggc agcgcttgag gatgttgatc aggtgatggc agccattgtt
    28381 ggcgctgctg ggctgttacc tacgcttgct gcgatccgcg cgggtaaaac cattttgctg
    28441 gccaataaag aatcactggt tacctgcgga cgtctgttta tggaggccgt aaagcagagc
    28501 aaagcgcaat tgttaccggt cgatagcgaa cataacgcca tttttcagag tttaccgcaa
    28561 cctatccagc ataatctggg atacgctgac cttgagcaaa atggcgtggt gtccatttta
    28621 cttaccgggt ctggtggccc tttccgtgag acgccattgc gtgatttggc aacaatgacg
    28681 ccggatcaag cctgccgtca tccgaactgg tcgatggggc gtaaaatttc tgtcgattcg
    28741 gctaccatga tgaacaaagg tctggaatac attgaagcgc gttggctgtt taacgccagc
    28801 gccagccaga tggaagtgct gattcacccg cagtcagtgc ttcactcaat ggtgcgctat
    28861 caggacggca gtgttctggc gcagctgggg gaaccggata tgcgtacgcc aattgcccac
    28921 accatggcat ggccgaatcg cgtgaactct ggcgtgaagc cgctcgattt ttgcaaacta
    28981 agtgcgttga catttgccgc accggattat gatcgttatc catgcctgaa actggcgatg
    29041 gaggcgttcg aacaaggtca ggcagcgacg acagcattga atgccgcaaa cgaaatcacc
    29101 gttgctgctt ttcttgcgca acaaatccgc tttacggata tcgctgcgtt gaatttatcc
    29161 gtactggaaa aaatggatat gcgcgaacca caatgtgtgg acgatgtgtt atctgttgat
    29221 gcgaacgcgc gtgaagtcgc cagaaaagag gtgatgcgtc tcgcaagctg aggataatcc
    29281 ggctacagag agtcgcgcta tttgttagcg tagggcttca gtgatatagt ctgcgccatc
    29341 tgatcgtaag tagttggctt tataaggtca ggtatgccgt ggttttacac ggcttttttt
    29401 tgtataggct tcagtattcc tgagtaccgt aaaccctgtc agggaataaa aaacgcgtga
    29461 tgttgtctgc tactcaacca cttagcgaaa aattgccagc gcatggctgc cgtcatgttg
    29521 cgatcattat ggacggcaat ggtcgctggg caaaaaagca agggaagatt cgtgcctttg
    29581 ggcataaagc cggggcaaaa tccgtccgcc gggctgtctc ttttgcggcc aacaacggta
    29641 ttgaggcgtt aacgctgtat gcctttagta gtgaaaactg gaaccgacca gcgcaggaag
    29701 tcagtgcgtt aatggaactg tttgtgtggg cgctcgatag cgaagtaaaa agtctgcacc
    29761 gacataacgt gcgtctgcgt attattggcg ataccagtcg ctttaactcg cgtttgcaag
    29821 aacgtattcg taaatctgaa gcgctaacag ccgggaatac cggtctgacg ctgaatattg
    29881 cggcgaacta cggtggacgt tgggatatag tccagggagt caggcaactg gctgaaaagg
    29941 tgcagcaagg aaacctgcaa ccagatcaga tagatgaaga gatgctaaac cagcatgtct
    30001 gtatgcatga actggcccct gtagatttag taattaggac tgggggggag catcgcatta
    30061 gtaacttttt gctttggcaa attgcctatg ccgaacttta ctttacagat gttctctggc
    30121 ccgatttcga tgaacaagac tttgaagggg cgttaaatgc ctttgctaat cgagagcgtc
    30181 gtttcggcgg caccgagccc ggtgatgaaa cagcctgatg ggggtcgctt ttgctgaagt
    30241 atcgcctgat atctgctttt gtgttaatac ccgtcgtcat cgcggcgttg tttctgttgc
    30301 cgccggtggg gttcgccatc gtaacgctgg tggtctgcat gctggcagcg tgggaatggg
    30361 gacagcttag cggttttacc actcgttcgc agcgagtatg gttggcggtg ttatgcgggt
    30421 tattgttggc gctgatgctt tttctgttgc cggaatatca ccgaaatatt catcaaccgc
    30481 tggttgaaat ctcactttgg gcttcgttgg gttggtggat tgtcgcgcta ttgctggtgc
    30541 tgttttaccc aggttccgca gcaatctggc gtaactctaa aacattgcgc cttatttttg
    30601 gcgtgctaac cattgttccc ttcttctggg gcatgctggc gttacgggcc tggcactacg
    30661 acgagaatca ttacagtggc gcaatatggc tgctctatgt catgatcctg gtatggggcg
    30721 ctgactctgg cgcatatatg tttggcaaat tgtttggtaa acataagctg gcaccgaagg
    30781 tttctccggg taaaacctgg caaggcttta taggtggact agcgaccgca gcggtaatct
    30841 catggggtta tggcatgtgg gcgaatctcg acgtcgctcc cgtcacctta ctcatttgct
    30901 ctattgtcgc agcgttagcc tcagtgctcg gcgatctgac cgagagtatg tttaagcgtg
    30961 aagcaggaat taaggacagc ggtcatttaa ttccaggaca cggtggtatt ttagatcgta
    31021 ttgatagcct gacggctgcg gtaccggtct ttgcttgctt gttgttactg gtattcagga
    31081 cgctttaacg gaaggtaata tgctgagttt tctctgggat ttggcttcgt tcatcgttgc
    31141 actgggtgta cttatcaccg tgcatgaatt tggtcatttc tgggttgccc ggcgttgtgg
    31201 tgttcgcgtt gagcgtttct caatagggtt tggtaaggcg ctctggcggc gaactgataa
    31261 gctcggcacc gaatatgtta tcgccctgat cccgctgggc ggttatgtca aaatgctgga
    31321 tgagcgcgca gaaccggtcg ttccggaact ccgccaccat gccttcaata ataaatctgt
    31381 cggccaacga gcggcgatta ttgccgcagg tccggttgca aacttcattt ttgctatctt
    31441 tgcctactgg ctggttttta ttattggtgt gcctggcgta cgtccggtgg ttggtgaaat
    31501 agcagccaat tcgatagctg cggaagcaca aattgcacca ggtacggaac taaaagccgt
    31561 agatggtatc gaaacgcctg attgggatgc cgtgcgtttg cagttggtcg ataaaattgg
    31621 cgatgaaagc accaccatta cagtagcgcc atttggcagc gaccaacggc gggatgtaaa
    31681 gctcgattta cgtcactggg cgtttgagcc tgataaagaa gatccggtat cttcgctggg
    31741 gattcgtcct cgtgggccgc aaattgaacc tgtactggaa aatgtgcaac caaactcggc
    31801 ggcaagcaag gcaggtttgc aagcaggcga caggatcgtt aaagtcgatg gtcagccctt
    31861 aacgcagtgg gtgacctttg tgatgcttgt ccgggataac ccgggtaaat ccttagcgtt
    31921 agaaatcgaa aggcagggaa gtcccttgtc tttgacatta atcccggaga gtaaaccggg
    31981 taatggtaaa gcgattggtt ttgtcggtat tgagccgaaa gtcattcctt tgccagatga
    32041 gtataaagtt gtacgccagt atgggccgtt caacgccatc gtcgaagcca cggacaaaac
    32101 gtggcagctg atgaagctga cggtcagtat gctgggaaaa ttgatcaccg gtgatgtgaa
    32161 actgaacaac ctcagtgggc cgatctctat cgccaagggg gctgggatga cagcggaact
    32221 cggggtagtt tattacctgc cgtttcttgc gcttattagc gtgaacttag ggataattaa
    32281 cctgtttccg ttgcccgtac ttgacggggg gcatctgctg ttccttgcga tcgaaaagat
    32341 caagggcgga ccggtatccg agcgggttca agacttttgt tatcgcattg gctcgattct
    32401 gctggtgctg ttaatggggc ttgcactttt caatgatttc tctcggttat gagagttagt
    32461 taggaagaac gcataataac gatggcgatg aaaaagttgc tcatagcgtc gctgctgttt
    32521 agcagcgcca ccgtatacgg tgctgaaggg ttcgtagtga aagatattca tttcgaaggc
    32581 cttcagcgtg tcgccgttgg tgcggccctc ctcagtatgc cggtgcgcac aggcgacacg
    32641 gttaatgatg aagatatcag taataccatt cgcgctctgt ttgctaccgg caactttgag
    32701 gatgttcgcg tccttcgtga tggtgatacc cttctggttc aggtaaaaga acgtccgacc
    32761 attgccagca ttactttctc cggtaacaaa tcggtgaaag atgacatgct gaagcaaaac
    32821 ctcgaggctt ctggtgtgcg tgtgggcgaa tccctcgatc gcaccaccat tgccgatatc
    32881 gagaaaggtc tggaagactt ctactacagc gtcggtaaat atagcgccag cgtaaaagct
    32941 gtcgtgaccc cgctgccgcg caaccgtgtt gacctaaaac tggtgttcca ggaaggtgtg
    33001 tcagctgaaa tccagcaaat taacattgtt ggtaaccatg ctttcaccac cgacgaactg
    33061 atctctcatt tccaactgcg tgacgaagtg ccgtggtgga acgtggtagg cgatcgtaaa
    33121 taccagaaac agaaactggc gggcgacctt gaaaccctgc gcagctacta tctggatcgc
    33181 ggttatgccc gtttcaacat cgactctacc caggtcagtc tgacgccaga taaaaaaggt
    33241 atttacgtca cggtgaacat caccgaaggc gatcagtaca agctttctgg cgttgaagtg
    33301 agcggcaacc ttgccgggca ctccgctgaa attgagcagc tgactaagat cgagccgggt
    33361 gagctgtata acggcaccaa agtgaccaag atggaagatg acatcaaaaa gcttctcggt
    33421 cgctatggtt atgcctatcc gcgcgtacag tcgatgcccg aaattaacga tgccgacaaa
    33481 accgttaaat tacgtgtgaa cgttgatgcg ggtaaccgtt tctacgtgcg taagatccgt
    33541 tttgaaggta acgatacctc gaaagatgcc gtcctgcgtc gcgaaatgcg tcagatggaa
    33601 ggtgcatggc tggggagcga tctggtcgat cagggtaagg agcgtctgaa tcgtctgggc
    33661 ttctttgaaa ctgtcgatac cgatacccaa cgtgttccgg gtagcccgga ccaggttgat
    33721 gtagtctaca aggtgaaaga acgtaacact ggtagcttca acttcgggat tggttacggt
    33781 actgaaagtg gcgtgagctt ccaggctggc gtgcaacagg ataactggtt aggtacaggt
    33841 tatgctgttg gtatcaacgg gaccaaaaac gattaccaga cctatgctga actgtcggta
    33901 accaacccgt acttcaccgt agatggcgta agcctcggtg gtcgtctctt ctataatgac
    33961 ttccaggcag atgacgccga cctgtccgac tataccaaca agagttatgg tacagacgtg
    34021 acgttgggct tcccgattaa cgaatataac tcgctgcgtg caggtctggg ttatgtacat
    34081 aactccctgt ccaacatgca gcctcaggtt gcgatgtggc gttatctgta ctctatgggt
    34141 gaacatccga gcacctctga tcaggataac agcttcaaaa cggacgactt cacgttcaac
    34201 tatggttgga cctataacaa gcttgaccgt ggttacttcc cgacagatgg ttcacgtgtc
    34261 aacctgaccg gtaaagtgac cattcctgga tcggataacg aatactacaa agtgacgtta
    34321 gacacggcga cttatgtgcc gatcgatgac gatcacaaat gggttgttct ggggcgtacc
    34381 cgctggggtt atggtgatgg tttaggcggc aaagagatgc cgttctacga gaacttctat
    34441 gccggtggtt ccagcaccgt gcgtggcttc cagtccaata ccattggtcc gaaagcagtt
    34501 tacttcccgc atcaggccag taattatgat ccggactatg attacgaatg tgcgactcag
    34561 gacggcgcaa aagacctgtg taaatcggat gatgctgtag gcggtaacgc catggcggtt
    34621 gccagcctcg agttcatcac cccgacgccg tttattagcg ataagtatgc taactcggtt
    34681 cgtacttcct tcttctggga tatgggtacc gtttgggata caaactggga ttccagccaa
    34741 tattctggat atccggacta tagtgatcca agcaatatcc gtatgtctgc gggtatcgca
    34801 ttacaatgga tgtccccatt ggggccgttg gtgttctcct acgcccagcc gttcaaaaag
    34861 tacgatggag acaaggcaga acagttccag tttaacatcg gtaaaacctg gtaagtgttc
    34921 tccacaaagg aatgtagtgg tagtgtagcg atgactttag gcgatcaata taagatcgcc
    34981 gggccacgca aagaactgca ccctccggtg caaatgggat ggtaaggagt ttattgtgaa
    35041 aaagtggtta ttagcggcag gtctcggttt agcactggca acttctgctc aggcggctga
    35101 caaaattgca atcgtcaaca tgggcagcct gttccagcag gtagcgcaga aaaccggtgt
    35161 ttctaacacg ctggaaaatg agttcaaagg ccgtgccagc gaactgcagc gtatggaaac
    35221 cgatctgcag gctaaaatga aaaagctgca gtccatgaaa gcgggcagcg atcgcactaa
    35281 gctggaaaaa gacgtgatgg ctcagcgcca gacttttgct cagaaagcgc aggcttttga
    35341 gcaggatcgc gcacgtcgtt ccaacgaaga acgcggcaaa ctggttactc gtatccagac
    35401 tgctgtgaaa tccgttgcca acagccagga tatcgatctg gttgttgatg caaacgccgt
    35461 tgcttacaac agcagcgatg taaaagacat cactgccgac gtactgaaac aggttaaata
    35521 agtaatgcct tcaattcgac tggctgattt agcgcagcag ttggatgcag aactacacgg
    35581 tgatggcgat atcgtcatca ccggcgttgc gtccatgcaa tctgcacaaa caggtcacat
    35641 tacgttcatg gttaacccaa aataccgtga gcatttaggc ttgtgccagg cgtccgcggt
    35701 tgtcatgacc caggacgatc ttcctttcgc gaaaagtgcc gcgctggtag tgaagaatcc
    35761 ctacctgact tacgcgcgca tggcgcaaat tttagatacc acgccgcagc ccgcgcagaa
    35821 cattgcaccc agtgcggtga tcgacgcgac ggcgaagctg ggtaacaacg tatcgattgg
    35881 cgctaacgcg gtgattgagt ccggcgttga actgggcgat aacgtgatta tcggtgccgg
    35941 ttgcttcgta ggtaaaaaca gcaaaatcgg tgcaggttcg cgtctctggg cgaacgtaac
    36001 catttaccat gagatccaga tcggtcagaa ttgcctgatc cagtccggaa cagtggtagg
    36061 cgcagacggc tttggttatg ccaacgatcg tggtaactgg gtgaagatcc cacagattgg
    36121 tcgcgtaatt attggcgatc gcgtggagat cggtgcctgc acaaccatcg atcgcggcgc
    36181 gctggatgac actattattg gcaatggcgt gatcattgat aaccagtgcc agattgcaca
    36241 taacgtcgtg attggcgaca atacggcggt tgccggtggc gtcattatgg cgggcagcct
    36301 gaaaattggt cgttactgca tgatcggcgg agccagcgta atcaacgggc atatggaaat
    36361 atgcgacaaa gtgacggtta cgggcatggg tatggtgatg cgtcccatca ctgaaccagg
    36421 cgtctattcc tcaggcattc cgctgcaacc caacaaagtc tggcgcaaaa ccgctgcact
    36481 ggtgatgaac attgatgaca tgagcaagcg tctgaaatcg cttgagcgca aggttaatca
    36541 acaagactaa cgttccatct tttgttcgcc aaactttacg gcctgtctca ttcttacgat
    36601 tgcggcaggc cgtgttatta ttgtcgtttc ttatattttg acaggaagag tatcttgact
    36661 actaacactc atactctgca gattgaagag attttagaac ttctgccgca ccgtttcccg
    36721 ttcttactgg tggatcgcgt gctggatttt gaagaaggtc gttttctgcg cgcagtaaaa
    36781 aatgtctctg tcaatgagcc attcttccag ggccatttcc ctggaaaacc gattttcccg
    36841 ggtgtgctga ttctggaagc aatggcacag gcaacaggta ttctggcgtt taaaagcgta
    36901 ggaaaactgg aaccgggtga gctgtactac ttcgctggta ttgacgaagc gcgcttcaag
    36961 cgcccggtcg tgcctggcga tcaaatgatc atggaagtca ctttcgaaaa aacgcgccgc
    37021 ggcctgaccc gttttaaagg ggttgctctg gtcgatggta aagtagtttg cgaagcaacg
    37081 atgatgtgtg ctcgtagccg ggaggcctga tacgtgattg ataaatccgc ctttgtgcat
    37141 ccaaccgcca ttgtggaaga gggcgcgtca attggcgcga acgcacacat tggtcctttt
    37201 tgtatcgttg gaccccatgt cgaaattggt gagggtaccg tactgaaatc tcacgttgtc
    37261 gtgaatggtc atactaaaat tggccgcgat aatgagattt atcagttcgc ctccatcggc
    37321 gaagttaacc aggatctgaa atatgctggc gaaccgaccc gtgtggaaat cggcgatcgt
    37381 aaccgcattc gcgaaagcgt caccattcat cgtggcacag tccagggcgg tggattgacg
    37441 aaggtgggca gcgacaactt actgatgatc aacgcgcaca ttgcgcacga ttgtacggta
    37501 ggtaaccgct gtattctcgc caacaacgca acgctggcgg gtcacgtatc ggttgacgac
    37561 ttcgcgatca tcggcggcat gaccgcagtc catcagttct gcatcattgg tgcgcacgtg
    37621 atggttggcg gctgctccgg tgtggcgcag gacgtccctc cttatgtcat tgcgcagggt
    37681 aaccacgcaa cgccgttcgg tgtcaatatc gaagggctga agcgccgcgg attcagccgt
    37741 gaggcgatta ccgctatccg caatgcgtat aagctgattt atcgtagcgg taaaacgctc
    37801 gatgaagtga aaccggaaat tgctgaactg gcggaaacat atccggaagt gaaagccttt
    37861 accgatttct ttgcacgctc aacgcgcggt ctgattcgtt aatgactgaa cagcgtccat
    37921 taacgattgc cctggtcgcc ggagaaacct ccggcgatat cctgggggcc ggtttaatcc
    37981 gcgctctgaa agaacgtgtg cccaacgccc gctttgttgg tgttgccggg ccacgaatgc
    38041 aggctgaagg ctgcgaagcc tggtacgaaa tggaagaact ggcggtgatg ggcattgttg
    38101 aagtgctcgg tcgtctgcgt cgcttactgc atattcgtgc cgatctgaca aagcgttttg
    38161 gcgaactgaa gccagatatt tttgttggta ttgatgcgcc tgacttcaat attactcttg
    38221 aaggtaacct caaaaagcag ggtatcaaaa ccattcatta cgtcagtcca tccgtctggg
    38281 cgtggcgaca gaaacgcgtt ttcaaaatag gcagagccac cgatctggtg ctcgcatttc
    38341 tgcctttcga aaaagcgttt tatgacaaat acaatgtacc gtgccgcttt atcggtcata
    38401 ccatggctga tgccatgccg ttagatccag ataaaaatgc cgcccgtgat gtgctgggga
    38461 tccctcacga tgcccactgc ctggcgttgc taccagggag ccgtggtgca gaagttgaaa
    38521 tgcttagtgc cgatttcctg aaaacggccc agcttttgcg ccagacatat ccggatctcg
    38581 aaatcgtggt gccactggtg aatgccaaac gccgcgagca gtttgaacgc atcaaagctg
    38641 aagtcgcgcc agacctttca gttcatttgc tggatgggat gggccgtgag gcgatggtcg
    38701 ccagcgatgc ggcgctactg gcgtcgggta cggcagccct ggagtgtatg ctggcgaaat
    38761 gcccgatggt ggtgggatat cgcatgaagc cttttacctt ctggttggcg aagcggctgg
    38821 tgaaaactga ttatgtctcg ctgccaaatc tgctggcgga tagagagtta gtcaaagaat
    38881 tattgcagga agagtgtgag ccgcaaaaac tggctgcggc gctgttaccg ctgttggcga
    38941 acgggaaaac cagccacgcg atgcacgata ccttccgtga actgcatcag cagatccgct
    39001 gcaatgccga tgagcaggcg gcacaagccg ttctggagtt agcacaatga tcgaatttgt
    39061 ttatccgcac acgcagctgg ttgcgggtgt ggatgaagtc ggacgcgggc cgttagttgg
    39121 cgcggtcgtc accgctgcgg tgatccttga cccggcgcgc ccgattgccg ggctgaatga
    39181 ttccaaaaag ctgagcgaaa aacgccgtct ggcgctctat gaagagatca aagagaaagc
    39241 gttgagctgg agtctgggcc gcgcggaacc ccacgaaatc gacgagctga acattcttca
    39301 tgcgaccatg ctggcgatgc agcgtgccgt cgctgggctg catattgcgc cggaatatgt
    39361 gttgattgat ggtaaccgct gcccgaaatt accgatgcct gcgatggctg tggtgaaagg
    39421 cgatagccgc gtaccggaaa tcagtgccgc gtctatcctg gcgaaagtga cgcgtgacgc
    39481 cgaaatggcg gcgctggata ttgttttccc gcaatatggt tttgcccaac acaaagggta
    39541 cccaaccgct tttcatctgg aaaaactggc tgaacacggc gcgaccgaac accatcggcg
    39601 cagctttggg cctgtcaaac gcgcaccggg acttgcgtcc tgattcttgt gtcgagatta
    39661 agtaaaccgg aatctgaaga tgtctgaacc acgtttcgta cacctgcggg tgcacagcga
    39721 ctactcgatg atcgatggcc tggccaaaac cgcaccgttg gtaaaaaagg cggcggcgtt
    39781 gggtatgcca gcactggcga tcaccgattt caccaacctt tgtggtctgg tgaagttcta
    39841 cggagcggga catggcgcag ggattaagcc tatcgtcggg gcagatttta acgtccagtg
    39901 cgacctgctg ggtgatgagt taacccacct gacggtactg gcggcgaaca ataccggcta
    39961 tcagaatctg acgttgctga tctcaaaagc gtatcagcgc gggtacggtg ccgccgggcc
    40021 gatcatcgat cgcgactggc ttatcgaatt aaacgaaggg ttgatccttc tttccggcgg
    40081 acgcatgggc gacgtcggac gcagtctttt gcgtggtaac agcgcgctgg tagatgagtg
    40141 tgtcgcgttt tatgaagaac acttcccgga tcgctatttt ctcgagctga tccgcaccgg
    40201 caggccggac gaagaaagct atctgcacgc ggcggtggaa ctggcggaag cgcgcggttt
    40261 gcccgtcgtg gcgaccaacg acgtgcgctt tatcgacagc agcgactttg acgcacacga
    40321 aatccgcgtc gcgatccacg acggctttac cctcgacgat cctaaacgcc cgcgtaacta
    40381 ttcgccgcag caatatatgc gtagcgaaga ggagatgtgt gagctgtttg ccgacatccc
    40441 cgaagccctt gccaacaccg ttgagatcgc caaacgctgt aacgtaaccg tgcgtcttgg
    40501 tgaatacttc ctgccgcagt tcccgaccgg ggacatgagc accgaagatt atctggtcaa
    40561 gcgtgcaaaa gagggcctgg aagagcgtct ggccttttta ttccctgatg aggaagaacg
    40621 tcttaagcgc cgcccggaat atgacgaacg tctggagact gaacttcagg ttatcaacca
    40681 gatgggcttc ccgggctact tcctcatcgt tatggaattt atccagtggt cgaaagataa
    40741 cggcgtaccg gtagggccag gccgtggctc cggtgcgggt tcactggtgg cctacgcgct
    40801 gaaaatcacc gacctcgatc cgctggaatt tgacctgctg ttcgaacgtt tccttaaccc
    40861 ggaacgtgtc tccatgcctg acttcgacgt tgacttctgt atggagaaac gcgatcaggt
    40921 tatcgaacac gtggcggaca tgtacggtcg tgatgcggta tcgcagatca tcaccttcgg
    40981 tacaatggcg gcgaaagcgg tgatccgcga cgtaggccgc gtgctggggc atccgtacgg
    41041 ctttgtcgat cgtatctcga aactgatccc gcccgatccg gggatgacgc tggcgaaagc
    41101 gtttgaagcc gagccgcagc tgccggaaat ctacgaagcg gatgaagaag ttaaggcgct
    41161 gatcgacatg gcgcgcaaac tggaaggggt cacccgtaac gccggtaagc acgccggtgg
    41221 ggtggttatc gcgccgacca aaattaccga ttttgcgccg ctttattgcg atgaagaggg
    41281 caaacatccg gtcacccagt ttgataaaag cgacgttgaa tacgccgggc tggtgaagtt
    41341 cgacttcctt ggtttgcgta cgctcaccat catcaactgg gcgctggaga tgatcaacaa
    41401 gcggcgggcg aagaatggcg agccaccgct ggatatcgcc gcgatcccgc tggatgacaa
    41461 gaagagcttc gacatgctgc aacgctcgga aaccacggcg gtattccagc ttgaatcgcg
    41521 cggcatgaag gacctgatca agcgtctgca acctgactgc ttcgaagata tgatcgcact
    41581 ggtggcactg ttccgccccg gtccgttgca atcagggatg gtggataact ttatcgaccg
    41641 taaacatggt cgtgaagaga tctcctatcc ggacgtacag tggcagcatg aaagcctgaa
    41701 accggtactg gagccaacct acggcattat cctgtatcag gaacaggtca tgcagattgc
    41761 gcaggtgctt tctggttata ccctcggtgg cgcggatatg ctgcgtcgtg cgatgggtaa
    41821 gaaaaagccg gaagagatgg ctaagcaacg ttctgtattt gctgaaggtg cagaaaagaa
    41881 cggaatcaac gctgaactgg cgatgaaaat cttcgacctg gtggagaaat tcgctggtta
    41941 cggatttaac aaatcgcact ctgcggccta tgctttggtg tcatatcaaa cgttatggct
    42001 gaaagcgcac tatcctgcgg agtttatggc ggcggtaatg accgccgata tggacaacac
    42061 cgagaaggtg gtgggtctgg tggatgagtg ctggcggatg gggctgaaaa tcctgccacc
    42121 agatataaac tccggtcttt accatttcca cgtcaacgac gacggcgaaa tcgtgtatgg
    42181 tattggcgcg atcaaagggg tcggtgaagg tccgattgag gccatcatcg aagcccgtaa
    42241 taaaggcggc tacttccgcg aactgtttga tctctgcgcc cgtaccgaca ccaaaaagtt
    42301 gaaccgtcgc gtgctggaaa aactgatcat gtccggggcg tttgaccgtc ttgggccaca
    42361 tcgcgcagcg ctgatgaact cgctgggcga tgcgttaaaa gcggcagatc aacacgcgaa
    42421 agcggaagct atcggtcagg ccgatatgtt cggcgtgctg gccgaagagc cggaacaaat
    42481 tgaacaatcc tacgccagct gccaaccgtg gccggagcag gtggtattag atggggaacg
    42541 tgaaacgtta ggcctgtacc tgaccggaca ccctatcaac cagtatttaa aagagattga
    42601 gcgttatgtc ggaggcgtaa ggctgaaaga catgcacccg acagaacgtg gtaaagtcat
    42661 cacggctgcg gggctcgttg ttgccgcgcg ggttatggtc accaagcgcg gcaatcgtat
    42721 cggtatctgc acgctggatg accgttccgg gcggctggaa gtgatgttgt ttactgacgc
    42781 cctggataaa taccagcaat tgctggaaaa agaccgcata cttatcgtca gcggacaggt
    42841 cagctttgat gacttcagcg gtgggcttaa aatgaccgct cgcgaagtga tggatattga
    42901 cgaagcccgg gaaaaatatg ctcgcgggct tgctatctcg ctgacggaca ggcaaattga
    42961 tgaccagctt ttaaaccgac tccgtcagtc tctggaaccc caccgctctg ggacaattcc
    43021 agtacatctc tactatcaga gggcggatgc acgcgcgcgg ttgcgttttg gcgcgacgtg
    43081 gcgtgtctct ccgagcgatc gtttattaaa cgatctccgt ggcctcattg gttcggagca
    43141 ggtggaactg gagtttgact aatacaggaa tactatgagt ctgaatttcc ttgattttga
    43201 acagccgatt gcagagctgg aagcgaaaat cgattctctg actgcggtta gccgtcagga
    43261 tgagaaactg gatattaaca tcgatgaaga agtgcatcgt ctgcgtgaaa aaagcgtaga
    43321 actgacgcgt aaaatcttcg ccgatctcgg tgcatggcag attgcgcaac tggcacgcca
    43381 tccacagcgt ccttataccc tggattacgt tcgcctggca tttgatgaat ttgacgaact
    43441 ggctggcgac cgcgcgtatg cagacgataa agctatcgtc ggtggtatcg cccgtctcga
    43501 tggtcgtccg gtgatgatca ttggtcatca aaaaggtcgt gaaaccaaag aaaaaattcg
    43561 ccgtaacttt ggtatgccag cgccagaagg ttaccgcaaa gcactgcgtc tgatgcaaat
    43621 ggctgaacgc tttaagatgc caatcatcac ctttatcgac accccggggg cttatccggg
    43681 cgtgggcgca gaagagcgcg gtcagtctga agccattgca cgcaacctgc gtgaaatgtc
    43741 tcgccttagc gtaccgacta tttgtaccgt tatcggtgaa ggtggttctg gcggcgcgct
    43801 ggcgattggc gtgggcgata aagtgaatat gctgcaatac agcacctatt ccgttatctc
    43861 gccggaaggt tgtgcgtcca ttctgtggaa gagcgccgac aaagcgccgc tggcggctga
    43921 agcgatgggt atcatcgctc cgcgtctgaa agagctgaaa ctgatcgact ccatcatccc
    43981 ggaaccgctg ggtggtgctc accgtaaccc ggaagcgatg gcggcatcgt tgaaagcgca
    44041 actgctggcg gatctggccg atctcgatgt gttaagcact gaagatttaa aaaatcgtcg
    44101 ttatcagcgc ctgatgagct acggttacgc gtaattcgca aaagttctga aaaagggtca
    44161 cttcggtggc ccttttttat cgccacggtt tgagcaggct atgattaagg aaggattttc
    44221 caggaggaac acatgaacat cattgccatt atgggaccgc atggcgtctt ttataaagat
    44281 gagcccatca aagaactgga gtcggcgctg gtggcgcaag gctttcagat tatctggcca
    44341 caaaacagcg ttgatttgct gaaatttatc gaacataacc ctcgaatttg cggcgtgatt
    44401 tttgactggg atgagtacag tctcgattta tgcagcgata tcaatcaact taatgaatat
    44461 ctcccgcttt atgccttcat caacacccac tcgacgatgg atgtcagcgt gcaggatatg
    44521 cggatggcgc tctggttttt tgaatatgcg ctggggcagg cggaagatat cgccattcgt
    44581 atgcgtcagt acaccgacga atatcttgat aacattacac cgccgttcac gaaagccttg
    44641 tttacctacg tcaaagagcg gaagtacacc ttttgtacgc cggggcatat gggcggcacc
    44701 gcatatcaaa aaagcccggt tggctgcctg ttttatgatt ttttcggcgg gaataccctc
    44761 aaggctgatg tctctatttc ggtcaccgag cttggttcgt tgctcgacca caccgggcca
    44821 cacctggaag cggaagagta catcgcgcgg acttttggcg cggaacagag ttatatcgtt
    44881 accaacggaa catcgacgtc gaacaaaatt gtgggtatgt acgccgcgcc atccggcagt
    44941 acgctgttga tcgaccgcaa ttgtcataaa tcgctggcgc atctgttgat gatgaacgat
    45001 gtagtgccag tctggctgaa accgacgcgt aatgcgttgg ggattcttgg tgggatcccg
    45061 cgccgtgaat ttactcgcga cagcatcgaa gagaaagtcg ctgccaccac gcaggcacaa
    45121 tggccggttc atgcggtgat taccaactcc acctatgatg gcttgctcta caacaccgac
    45181 tggatcaaac agacgctgga tgtcccgtcg atccacttcg attccgcctg ggtgccgtac
    45241 acccattttc atccgatcta ccaggggaaa agtggtatga gcggcgagcg tgttgcggga
    45301 aaagtgatct tcgaaacgca atcgacccac aaaatgctgg cggcgttatc gcaggcttcg
    45361 ctgatccaca ttaaaggcga gtatgacgaa gaggcgttta acgaagcctt tatgatgcat
    45421 accaccacct cgcccagtta tcccattgtt gcttcggttg agacggcggc ggcgatgctg
    45481 cgtggtaatc cgggcaaacg gctgattaac cgttcggtgg agcgggcgct gcattttcgc
    45541 aaagaggtcc agcggttgcg ggaagagtct gacggttggt ttttcgatat ctggcaaccg
    45601 ccgcaggtgg atgaagccga atgctggccc gttgcgccag gcgaacagtg gcacggcttt
    45661 aacgatgcgg atgccgatca tatgtttctc gatccggtta aagtcactat tttgacaccg
    45721 gggatggacg aacagggcaa tatgagcgag gaggggatcc cggcggcgct ggtggcaaaa
    45781 ttcctcgacg aacgtgggat cgtagtagag aaaaccggcc cttataacct gctgtttctc
    45841 tttagtattg gtatcgataa aaccaaagca atgggattat tgcgtgggtt gacggaattt
    45901 aaacgctctt acgatctcaa cctgcggatc aaaaatatgc tacccgatct ctatgctgaa
    45961 gatcccgatt tctaccgcaa tatgcgtatt caggatctgg cgcaagggat ccataagctg
    46021 attcgtaaac acgatcttcc cggtttgatg ttgcgggcat tcgatacttt gccggaaatg
    46081 atcatgacgc cacatcaggc atggcagaga cagattaaag gtgaagtaga aaccattgcg
    46141 ctggaacaac tggtcggtag agtatcggca aatatgatcc tgccttatcc accgggcgta
    46201 ccgctgttga tgccaggaga aatgctgacc aaagagagcc gcacggtact cgattttcta
    46261 ctgatgcttt gttccgtagg gcaacattac cccggttttg aaacggatat tcacggcgcg
    46321 aaacaggacg aagacggcgt ttaccgagta cgagtcctaa aaatggcggg ataacttgcc
    46381 agagcggctt ccgggcgagt aacgtgctgt taacaaataa aggagacgtt atgctgggtt
    46441 taaaacaggt tcaccatatt gcgattattg cgacggatta tgcggtgagc aaagctttct
    46501 actgcgatat tcttggtttc acgctgcaaa gcgaagtcta tcgcgaagcg cgcgactcgt
    46561 ggaaagggga tttggcgctt aatgggcaat atgtgattga gcttttctct tttccgttcc
    46621 cgccggagcg ccccagccga ccggaagctt gcggcctgcg tcatctggct tttagcgttg
    46681 atgacatcga tgcggcagtg gcgcaccttg aaagccataa cgtgaagtgt gaagccatcc
    46741 gtgtcgatcc atacacgcaa aaacgcttca ccttctttaa cgatccggac gggctgccgt
    46801 tggaactgta tgagcagtaa ggcttgtcat cgccgcattt gcccggtaac gtgccgggca
    46861 ttgctactgt aaaatcgcac catcatgaca ctcacgctca atagacaact tctcacctca
    46921 cgccagattc tggtggcctt tagcggcggg cttgactcca ccgttctgct gcatcagttg
    46981 gtgcagtggc ggacggaaaa tctgggcgtc actctgcgcg ctattcatgt gcatcacggt
    47041 ttaagtgcca atgccgatgc ctgggttacg cattgcgaaa acgtctgcca acagtggcag
    47101 gtgccgctgg tggtcgaacg cgtgcaactt gcgcaagaag gactgggcat tgaggcccag
    47161 gcgcggcagg cgcgttatca ggcatttgcc cgcaccttgt tgcccggtga agtgctggtc
    47221 accgcgcaac atctcgacga tcaatgtgaa acttttctgc tggcgctaaa acgcggtagc
    47281 ggccctgccg ggctttcggc tatggcggaa gtttcggagt ttgccggaac acggcttatc
    47341 cgcccgttgc tcgcccgcac gcggggagaa ctggagcagt gggcattggc gcatggttta
    47401 cgctggattg aagacgaaag taatcaggac gactcatacg atcgtaactt tctgcgtctg
    47461 cgcgtagtgc cgttattaca gcagcgttgg ccgcattttg ccgaagcaac ggcccgcagc
    47521 gccgcacttt gcgctgaaca agagagtctg ttggatgaac tgctggcgga tgatttagca
    47581 cactgccaaa cgtcgcaggg ggcgctgcag attgcgccaa tgctggcgat gagtgatgcc
    47641 cgtcgcgcgg cgattatccg ccgctggctg gcagggaaga atgcaccgat gccttcccgc
    47701 gacgcgctgg tgcggatctg gcaggaagtg gcgctggcgc gggaagatgc ctccccctgt
    47761 ttacgcttgg gcgcgtttga aatccgccgc tatcagtcgc aactgtggtg gattaaatcc
    47821 gtcaccgggc aaagtgaaac cattgtgccg tggcagacgt ggctacaacc gctggaatta
    47881 ccggcggggc tgggaagcgt acagcttact gcgggaggca atattcgccc tccgcgtgca
    47941 gacgaagcgg tcagcgtgcg tttcaaagcg gcaggattgc tgcatattgt cgggcgcaac
    48001 ggcggacgta agctgaagaa aatctggcaa gagttaggcg tgccgccgtg gctacgtgac
    48061 accacgccac tgctgtttta tggcgaaacg ctgattgcgg cggcaggggt atttgtgacg
    48121 caagaaggtg tggctgaagg tgagaatggc gtaagttttg tctggcagaa aacgcttagt
    48181 taagtgaaag ccggataaga cgcatcaaac gtcgcatccg gcgaaagtca atcaggactc
    48241 gctcaccacc accgtaccga tttccgggtg gctaaagctg gtaattttat ccagacgtag
    48301 ctcacgggtt tcgccagcgg cctcgacgac cagatactcc acatttttgc gggagactaa
    48361 atcgctggct tttgcctgca atttttcgcc atctttcagc tcaagtgtca gcattaaatg
    48421 atgctggcag gcgagttcaa gattatcgta atcatcacaa ttgattggtt gatacgtatc
    48481 attcattgac ataatcgctc accagtaagt ttgccgcagc gtatgctgct ttttccctga
    48541 cagcctcaga aagggcgtcg tcggcagcca tttcattcag cactttcaaa acgcagccca
    48601 gcgcgtccgg aacgtatcct aagtctccgc tggcgatttc cgcgtaccgc ttgcgtatta
    48661 actcacaata tttttccaca tgccctcctg tcagcactct gacttaaccg tggatgcaag
    48721 tctaagccta cgaagataaa ctctgtttcg caaggtgact ataccacact catttctgca
    48781 atatcagcgc cgcaactgca cgtattccgt tacaatggcc tcctgattcg aaaggagttt
    48841 tcttatggcg cttaaagcga caatttataa agcgacggtt aatgtggccg atctcgaccg
    48901 caaccagttt ctcgatgcct ctctgacgct ggcgcgccac ccttcagaaa cccaggagcg
    48961 tatgatgctg cgcctgctgg cgtggctgaa atatgctgat gaacgtctgc aatttacccg
    49021 tggtttgtgt gccgatgatg agccggaagc gtggctgcgt aacgatcacc tgggcattga
    49081 tttgtggatt gagctggggc tgccggatga gcggcggatt aagaaagcct gcacccaggc
    49141 cgcagaagtg gcgctgttta cctataatag tcgggcggcg caaatctggt ggcagcaaaa
    49201 tcagagcaaa tgtgtgcagt ttgccaatct ttccgtctgg tatctggacg atgaacaact
    49261 ggcgaaagta agcgcctttg ccgatcgtac catgacgctg caggcaacga ttcaggatgg
    49321 cgtgatctgg ttatcggacg ataagaataa tctggaagtt aacttaaccg cctggcaaca
    49381 accttcatga ttgtgatttc ccgacatgtt gctattcccg atggtgagct tgagatcacc
    49441 gccattcgtg cgcagggcgc gggcgggcag catgttaata agacctcaac ggccattcat
    49501 ctgcgttttg acattcgggc gtccagcctg ccagagtatt acaaagagcg tctgctcgcc
    49561 gccagccatc atttgatcag cagtgatggc gtgattgtca ttaaggcaca ggaataccgc
    49621 agtcaggaac tgaaccgcga agccgcgctg gcccggctgg tggcagtgat taaagattta
    49681 acaacagaac aaaaagcccg acgacccacg cggcccaccc gtgcatcgaa agagcgcagg
    49741 ctggcatcga aagcacaaaa atcaagcgtg aaggcgatgc gcggcaaagt gcgcagcggt
    49801 cgggaataaa aagaaggaat ggatggtgaa aaaagcgata gtgacagcga tggctgtaat
    49861 cagcctcttt actctgatgg gatgtaataa ccgggccgaa gtcgatacgc tttctccagc
    49921 acaggctgcc gaactgaaac cgatgccgca aagttggcgc ggcgtgctgc cgtgtgctga
    49981 ttgcgaagga atcgaaacct ctctgttcct cgaaaaagac ggaacatggg tgatgaatga
    50041 gcgttatctc ggtgctcgtg aagaaccttc ctccttcgct tcctacggta catgggcgcg
    50101 aaccgctgac aagctggtat taaccgacag caaaggtgaa aagtcatatt atcgggccaa
    50161 aggcgatgcg ctggagatgc tcgatcgtga aggcaatccg attgaatcgc agttcaacta
    50221 tacgctggaa ccggcacaat ccagtttacc cctgacgccg atgaccctgc ggggcatgta
    50281 tttttatatg gctgatgtgg cgaccttcac tgattgcgcg accggaaaac gtttcatggt
    50341 agcgaataac gcagagctgg agcgtagcta cctggctgcg cgcggtcaca gtgaaaaacc
    50401 ggtgttactg tcagtagaag gtcactttac gcttgaggct aatccggata ctggtgcgcc
    50461 aaccaaagta ttagcgcccg atacggcggg taaattttac ccgaataagg attgcagtag
    50521 tttggggcag taaacctgct taaggcagaa acaaacgcaa
//
LOCUS       DAZKIV010000015        48887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZKIV010000015 DAZKIV010000000
VERSION     DAZKIV010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.015"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..1709
                     /gene="mqo"
                     /locus_tag="ACHOT6_000923"
     CDS             63..1709
                     /gene="mqo"
                     /locus_tag="ACHOT6_000923"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFP9946184.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(1858..2367)
                     /gene="eco"
                     /locus_tag="ACHOT6_000924"
     CDS             complement(1858..2367)
                     /gene="eco"
                     /locus_tag="ACHOT6_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFP9946185.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(2481..2645)
                     /locus_tag="ACHOT6_000925"
     CDS             complement(2481..2645)
                     /locus_tag="ACHOT6_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946186.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNCYKHIYIAINSPEI
                     FLLVCSLTRI"
     gene            2754..3248
                     /gene="napF"
                     /locus_tag="ACHOT6_000926"
     CDS             2754..3248
                     /gene="napF"
                     /locus_tag="ACHOT6_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFP9946187.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            3238..3501
                     /gene="napD"
                     /locus_tag="ACHOT6_000927"
     CDS             3238..3501
                     /gene="napD"
                     /locus_tag="ACHOT6_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFP9946188.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            3498..5984
                     /gene="napA"
                     /locus_tag="ACHOT6_000928"
     CDS             3498..5984
                     /gene="napA"
                     /locus_tag="ACHOT6_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HFP9946189.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            5991..6686
                     /gene="napG"
                     /locus_tag="ACHOT6_000929"
     CDS             5991..6686
                     /gene="napG"
                     /locus_tag="ACHOT6_000929"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFP9946190.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            6673..7536
                     /gene="napH"
                     /locus_tag="ACHOT6_000930"
     CDS             6673..7536
                     /gene="napH"
                     /locus_tag="ACHOT6_000930"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFP9946191.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            7533..7982
                     /gene="napB"
                     /locus_tag="ACHOT6_000931"
     CDS             7533..7982
                     /gene="napB"
                     /locus_tag="ACHOT6_000931"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFP9946192.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            7992..8594
                     /gene="napC"
                     /locus_tag="ACHOT6_000932"
     CDS             7992..8594
                     /gene="napC"
                     /locus_tag="ACHOT6_000932"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFP9946193.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLSGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            8607..9230
                     /gene="ccmA"
                     /locus_tag="ACHOT6_000933"
     CDS             8607..9230
                     /gene="ccmA"
                     /locus_tag="ACHOT6_000933"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFP9946194.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTGAA"
     gene            9227..9889
                     /gene="ccmB"
                     /locus_tag="ACHOT6_000934"
     CDS             9227..9889
                     /gene="ccmB"
                     /locus_tag="ACHOT6_000934"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFP9946195.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            9931..10668
                     /gene="ccmC"
                     /locus_tag="ACHOT6_000935"
     CDS             9931..10668
                     /gene="ccmC"
                     /locus_tag="ACHOT6_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFP9946196.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            10665..10874
                     /gene="ccmD"
                     /locus_tag="ACHOT6_000936"
     CDS             10665..10874
                     /gene="ccmD"
                     /locus_tag="ACHOT6_000936"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFP9946197.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            10871..11350
                     /gene="ccmE"
                     /locus_tag="ACHOT6_000937"
     CDS             10871..11350
                     /gene="ccmE"
                     /locus_tag="ACHOT6_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFP9946198.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            11347..13290
                     /gene="ccmF"
                     /locus_tag="ACHOT6_000938"
     CDS             11347..13290
                     /gene="ccmF"
                     /locus_tag="ACHOT6_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFP9946199.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAAAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            13287..13844
                     /gene="dsbE"
                     /locus_tag="ACHOT6_000939"
     CDS             13287..13844
                     /gene="dsbE"
                     /locus_tag="ACHOT6_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFP9946200.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            13841..14893
                     /gene="ccmH"
                     /locus_tag="ACHOT6_000940"
     CDS             13841..14893
                     /gene="ccmH"
                     /locus_tag="ACHOT6_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFP9946201.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(14928..15575)
                     /gene="narP"
                     /locus_tag="ACHOT6_000941"
     CDS             complement(14928..15575)
                     /gene="narP"
                     /locus_tag="ACHOT6_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFP9946202.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            15895..18486
                     /gene="yejO"
                     /locus_tag="ACHOT6_000942"
                     /pseudo
     CDS             15895..18486
                     /gene="yejO"
                     /locus_tag="ACHOT6_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(18590..18666)
                     /locus_tag="ACHOT6_000943"
     tRNA            complement(18590..18666)
                     /locus_tag="ACHOT6_000943"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18630..18632),aa:Pro,seq:ggg)
     gene            complement(18741..20501)
                     /gene="yejM"
                     /locus_tag="ACHOT6_000944"
     CDS             complement(18741..20501)
                     /gene="yejM"
                     /locus_tag="ACHOT6_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFP9946203.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWASFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(20521..20748)
                     /gene="yejL"
                     /locus_tag="ACHOT6_000945"
     CDS             complement(20521..20748)
                     /gene="yejL"
                     /locus_tag="ACHOT6_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFP9946204.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            20930..21937
                     /gene="yejK"
                     /locus_tag="ACHOT6_000946"
     CDS             20930..21937
                     /gene="yejK"
                     /locus_tag="ACHOT6_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFP9946205.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(22069..22359)
                     /gene="rplY"
                     /locus_tag="ACHOT6_000947"
     CDS             complement(22069..22359)
                     /gene="rplY"
                     /locus_tag="ACHOT6_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFP9946206.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVALNC"
     gene            complement(22484..24244)
                     /gene="radD"
                     /locus_tag="ACHOT6_000948"
     CDS             complement(22484..24244)
                     /gene="radD"
                     /locus_tag="ACHOT6_000948"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFP9946207.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHEHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            24393..25088
                     /gene="rsuA"
                     /locus_tag="ACHOT6_000949"
     CDS             24393..25088
                     /gene="rsuA"
                     /locus_tag="ACHOT6_000949"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFP9946208.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            25116..26306
                     /gene="bcr"
                     /locus_tag="ACHOT6_000950"
     CDS             25116..26306
                     /gene="bcr"
                     /locus_tag="ACHOT6_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFP9946209.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILAVAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            26639..26983
                     /gene="yejG"
                     /locus_tag="ACHOT6_000951"
     CDS             26639..26983
                     /gene="yejG"
                     /locus_tag="ACHOT6_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFP9946210.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(26987..28576)
                     /gene="yejF"
                     /locus_tag="ACHOT6_000952"
     CDS             complement(26987..28576)
                     /gene="yejF"
                     /locus_tag="ACHOT6_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFP9946211.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(28578..29603)
                     /gene="yejE"
                     /locus_tag="ACHOT6_000953"
     CDS             complement(28578..29603)
                     /gene="yejE"
                     /locus_tag="ACHOT6_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946212.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(29603..30697)
                     /gene="yejB"
                     /locus_tag="ACHOT6_000954"
     CDS             complement(29603..30697)
                     /gene="yejB"
                     /locus_tag="ACHOT6_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFP9946213.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSKYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(30698..32512)
                     /gene="yejA"
                     /locus_tag="ACHOT6_000955"
     CDS             complement(30698..32512)
                     /gene="yejA"
                     /locus_tag="ACHOT6_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFP9946214.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPIYSLGIDTWWYDVNKAAKLPSASKQGE"
     gene            complement(32594..34150)
                     /gene="pdeN"
                     /locus_tag="ACHOT6_000956"
     CDS             complement(32594..34150)
                     /gene="pdeN"
                     /locus_tag="ACHOT6_000956"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFP9946215.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(34331..34897)
                     /gene="mepS"
                     /locus_tag="ACHOT6_000957"
     CDS             complement(34331..34897)
                     /gene="mepS"
                     /locus_tag="ACHOT6_000957"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFP9946216.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(35309..36022)
                     /gene="lpxT"
                     /locus_tag="ACHOT6_000958"
     CDS             complement(35309..36022)
                     /gene="lpxT"
                     /locus_tag="ACHOT6_000958"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFP9946217.1"
                     /translation="MIKNLPQIVLLNIVGLTLFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILSGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(36061..37047)
                     /gene="yeiR"
                     /locus_tag="ACHOT6_000959"
     CDS             complement(36061..37047)
                     /gene="yeiR"
                     /locus_tag="ACHOT6_000959"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFP9946218.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(37165..38631)
                     /gene="yeiQ"
                     /locus_tag="ACHOT6_000960"
     CDS             complement(37165..38631)
                     /gene="yeiQ"
                     /locus_tag="ACHOT6_000960"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HFP9946219.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            complement(38854..39426)
                     /gene="yeiP"
                     /locus_tag="ACHOT6_000961"
     CDS             complement(38854..39426)
                     /gene="yeiP"
                     /locus_tag="ACHOT6_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFP9946220.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            39581..39835
                     /gene="yeiW"
                     /locus_tag="ACHOT6_000962"
     CDS             39581..39835
                     /gene="yeiW"
                     /locus_tag="ACHOT6_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9946221.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(39832..41013)
                     /gene="setB"
                     /locus_tag="ACHOT6_000963"
     CDS             complement(39832..41013)
                     /gene="setB"
                     /locus_tag="ACHOT6_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFP9946222.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            41381..42511
                     /gene="fruB"
                     /locus_tag="ACHOT6_000964"
     CDS             41381..42511
                     /gene="fruB"
                     /locus_tag="ACHOT6_000964"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFP9946223.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNSIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            42511..43449
                     /gene="fruK"
                     /locus_tag="ACHOT6_000965"
     CDS             42511..43449
                     /gene="fruK"
                     /locus_tag="ACHOT6_000965"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFP9946224.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            43466..45157
                     /gene="fruA"
                     /locus_tag="ACHOT6_000966"
     CDS             43466..45157
                     /gene="fruA"
                     /locus_tag="ACHOT6_000966"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFP9946225.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            45581..46522
                     /gene="psuK"
                     /locus_tag="ACHOT6_000967"
     CDS             45581..46522
                     /gene="psuK"
                     /locus_tag="ACHOT6_000967"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFP9946226.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            46510..47448
                     /gene="psuG"
                     /locus_tag="ACHOT6_000968"
     CDS             46510..47448
                     /gene="psuG"
                     /locus_tag="ACHOT6_000968"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFP9946227.1"
                     /translation="MSKLKISPELLQISPEVQEALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            47542..48792
                     /gene="psuT"
                     /locus_tag="ACHOT6_000969"
     CDS             47542..48792
                     /gene="psuT"
                     /locus_tag="ACHOT6_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9946228.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT        11711 a        12005 c        13284 g        11887 t
ORIGIN      
        1 taccgttgat gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca
       61 agatgaaaaa agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga
      121 tggcggcaaa agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg
      181 gcattatgag cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga
      241 ccatggtgga gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aataacgccg
      301 gaaccgggca ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagca
      361 tcagtattga aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg
      421 cgcaccaggt tgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc
      481 atatgagctt tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt
      541 tgcaacaaag ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag
      601 agtgggcacc gttagtgatg gaagggcgcg atccacaaca gaaagtagcg gcaacacgca
      661 cagaaattgg cactgatgtg aactatggcg agatcacccg ccagttaatt gcttccttac
      721 agaagaaatc taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg
      781 acgataacac ctggaccgtt accgttgccg atctgaaaaa cggcactgca cagaacatcc
      841 gcgccaaatt tgtctttatc ggcgcgggcg gtgcggcgct gaagctatta caggaatcgg
      901 ggattccgga agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg
      961 aaaacccgga cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg
     1021 caccaccgat gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc
     1081 tgtttgggcc atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc
     1141 tgatgagttc caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt
     1201 tcgatctggt gaaatatctg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag
     1261 cgttgaaaga gtactatccg caggcaaaaa aagaggactg gcgtttgtgg caagcggggc
     1321 agcgcgtgca gattatcaag cgtgatgccg agaaaggtgg cgtactgcgt ctgggtactg
     1381 aagtggtcag tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt
     1441 caaccgccgc gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca
     1501 gcccgcaatg gcaggctacg ttgaaagcga tcgttccgtc ttatggacgc aagctgaacg
     1561 gtgatgtagc ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg gggctgaaat
     1621 acgacaagcc gcaggcagca gatagtacgc caaaaccgca gttgaaaccg caacccgttc
     1681 aaaaagaagt ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga
     1741 tgtggcgtat gctgataaga cgcgccagcg tcgcatcagg caccggctcg ggcgttagat
     1801 gtcggatgcg gcgtgaccgc cttattcggc ctacggggtg ccgcacttca cagcatttta
     1861 gcgaactacc gcgttgtcaa ttttctcttc cgccttccag acgcggtact tcacatctac
     1921 attgtctggc gtatacacca cgatcggcag cttgctgttg taacgcagca ttccagcatc
     1981 gcccagatac gcggtgacaa atttcttctc tttcttgcca tccgggcagg ccatcatcgt
     2041 ggaaaccggc gagctaactt tatcaaagac ataataatca tagccccagc cttccagcgt
     2101 tttgttttcc agcttcccgc cgagacgatg caaattgcaa tcgacttcca gcgtctgacc
     2161 gattaacagt tctactttca gggtagattc atcttcttgc ggggttaact gaatcacctg
     2221 acgcttcatc cctttttcag cttgtggata aggcgcgatt ttttccagtg gctggacgct
     2281 ttctgccgcc caggcggaag tggtagcgaa agcggcaaac aatactgcag gtagaatggt
     2341 cttcattttt ttgctttcct tcttcattta taggttttca ccgatgatgt cgctgaaggt
     2401 taacatattc atcagattta ttaatttgct ttatgaataa cttaaagaac attcagacaa
     2461 ttttacctgc tcttttagtg ttaaattctg gtgagggagc aaaccagcag aaatatctct
     2521 ggtgaattaa tcgctatata aatatgttta taacaatttg aaatgtgagc aaaagcccgt
     2581 ttttgccacg cttcccgcca ctcttttgat cctgctacag gttttacccc gatcggggta
     2641 tgcatctttg acacatcctt taatatctta gcggctataa aaatggctta ttaattatgc
     2701 ggtttatttg gccgctctca attttcagag cgcgttaatg atggaaggtc aatgtgaaga
     2761 ttgatgcatc ccgtcggggc atactcactg gtcgctggcg caaagccagt aacggtatcc
     2821 gtccgccttg gtccggtgat gaatctcatt ttctgaccca ttgcacccgt tgcgacgcct
     2881 gtattaatgc ttgtgaaaac aacattctgc aacgcggcgc gggcggctat ccgagcgtta
     2941 atttcaaaaa taacgagtgc agcttctgtt acgcctgcgc gcaggcttgc cccgaatcgc
     3001 tattttctcc gcgccacacc agggcctggg atttgcagtt caccattggg gacgcgtgcc
     3061 tggcgtatca gtcagttgaa tgccgccgct gtcaggatag ctgtgagcca atggcaatta
     3121 tctttcgccc gacgctctcc gggatctacc agccgcaact taatagccaa ctttgcaacg
     3181 gatgtggagc atgtgcagcc agctgtccgg tatcagccat aactgcggag tatctccatg
     3241 cacactaact ggcaagtttg cagcctggtc gtgcaggcca aaagcgaacg aatttcagac
     3301 atcagcaccc aactgaacgc ctttcccggc tgtgaagttg ctgtcagcga cgcgccgagc
     3361 ggtcagttga ttgtggtggt ggaagcagaa gacagcgaaa cgctgatcca aaccattgag
     3421 tcagtacgca acgtagaggg cgtgctggcg gtgtcgctgg tttatcacca gcaggaagag
     3481 caaggtgagg aaacaccatg aaactcagtc gtcgtagctt tatgaaagct aacgccgttg
     3541 cggccgctgc ggcggctgcc ggtctcagcg tgccgggcgt tgcccgcgcc gttgttggtc
     3601 agcaggaagc cattaaatgg gataaagcgc cgtgccgttt ctgcggtact ggttgcggcg
     3661 ttctggtcgg aacgcagcag gggcgtgtgg tggcctgtca gggcgacccg gacgcaccgg
     3721 ttaaccgtgg cctgaactgc attaagggct atttcctgcc caagatcatg tacggtaaag
     3781 accgtttgac gcagccgctg ctgcgtatga aaaacggtaa atatgacaaa gaaggcgaat
     3841 ttaccccaat cacctgggat caggccttcg atgtgatgga agagaaattc aaaaccgccc
     3901 tgaaagaaaa aggaccggaa tcgatcggta tgttcggttc tggtcagtgg actatctggg
     3961 aaggttatgc cgcgtccaag ctgttcaaag cgggcttccg ttcgaacaac atcgacccga
     4021 acgcacgtca ctgtatggcg tcggcagtag ttggctttat gcgtaccttt ggtatggatg
     4081 agccgatggg ctgctatgac gacatcgagc aggctgacgc gtttgtgctg tggggcgcta
     4141 acatggcgga gatgcacccg atcctctggt cacgcatcac taaccgtcgt ctctctaacc
     4201 agaacgtcac cgtggcggtg ctttctacct accagcatcg tagcttcgag ctggcggata
     4261 acggcatcat ctttacgcca cagtctgacc tggtgatcct gaactacatc gccaactata
     4321 tcattcaaaa caatgcgata aatcaggact tcttcagcaa gcacgttaac ctgcgcaaag
     4381 gggcgacgga catcggctac ggtttacgtc cgacccatcc gctggaaaaa gcagcgaaga
     4441 atccgggttc tgacgcctcc gaaccgatga gctttgaaga ttacaaagcc ttcgttgccg
     4501 agtatacgct ggaaaaaact gccgaaatga ccggcgtgcc gaaagaccag ttagaacaac
     4561 tggcgcagct gtatgccgat ccgaacaaga aagtcatctc ctactggacg atgggcttca
     4621 accagcatac tcgtggcgtg tgggccaaca acctggtcta caacctgcac ctgctgaccg
     4681 gcaaaatttc ccagccgggt tgcggtccgt tctccctgac cgggcagcct tccgcgtgtg
     4741 gtactgcgcg tgaagtgggc acctttgctc accgtctgcc tgcggacatg gtggtgacta
     4801 acgagaaaca ccgtgatatt tgcgagaaga agtggaatat cccgagcggc accattccgg
     4861 cgaaaatcgg tctgcacgcg gtggcacaag accgtgcact gaaagacggc aagctgaatg
     4921 tttactggac catgtgtacc aacaacatgc aggccgggcc gaacattaat gaagagcgta
     4981 tgccgggctg gcgcgatccg cgcaacttca tcatcgtctc cgatccgtat ccgacagtca
     5041 gtgcgctggc agccgacttg atcctgccga ccgcaatgtg ggtagagaaa gagggcgctt
     5101 acggtaacgc cgaacgccgt actcagttct ggcgtcagca ggtacaggcg ccaggcgaag
     5161 cgaaatcgga tctctggcag ttagtccagt tctcccgccg cttcaaaact gaagaagtat
     5221 ggccggaaga gctgctggcg aagaaaccgg aactgcgtgg caaaacgctg tacgaagttc
     5281 tgtatgccac ccccgaagtg agcaaatacc ctgtatccga actggcggaa gatcagctga
     5341 acgatgaatc ccgcgagctg ggcttctatc tgcaaaaagg gctgttcgaa gagtacgcat
     5401 ggtttggtcg cggtcacggt cacgatctgg caccgttcga tgactaccac aaagcgcgcg
     5461 gtctgcgctg gccggtggtg aacggtaaag aaacgcagtg gcgttacagc gaaggtaacg
     5521 acccgtacgt gaaagcgggc gaaggctata agttctacgg taaaccggat ggcaaagcgg
     5581 tgatcttcgc actgccgttc gaaccggcgg cggaagcacc ggatgaagag tacgacctgt
     5641 ggctctctac cggacgcgtt ctggagcact ggcacaccgg cagtatgact cgccgtgtgc
     5701 cggaactgca ccgcgccttc ccggaagcgg tcttgtttat tcacccgctg gatgcgaaag
     5761 cgcgcgatct gcgccgtggc gacaaagtga aagtggtttc tcgccgtggc gaagtgatct
     5821 cgattgttga aacgcgcggt cgtaaccgtc cgccacaggg cctggtgtac atgccgttct
     5881 tcgacgccgc acagctggtt aacaaactga cgctggatgc gaccgatccg ctctcgaaag
     5941 agacggactt caagaagtgc gcagtcaaac tggagaaggt gtaagccatt atgtcccggt
     6001 cagcgaaacc tcaaaatggt cgccgccgct ttctgcgcga tgtcgttcgc acagcaggcg
     6061 ggctggctgc cgtgggtgtg gcgctggggt tacaacagca aaccgcacgc gcatctggcg
     6121 tgcggttgcg cccgcccgga gccataaacg agaacgcctt tgccagtgcc tgtgtgcgtt
     6181 gcggtcagtg tgttcaggct tgcccttacg acaccttaaa actggcgacg ctggcctctg
     6241 gtctgtcggc gggcacgcca tattttgtcg cacgggatat tccttgcgaa atgtgtgagg
     6301 acattccgtg cgccaaagtg tgcccaagcg gtgcgctgga tcgtgagatt gaatcgatcg
     6361 acgacgcgcg gatggggctg gcggtactgg tggaccagga aaactgtctc aactttcagg
     6421 ggctgcgctg cgatgtttgt tatcgcgaat gcccgaaaat tgatgaggcc atcaccctgg
     6481 agctggagcg caacacgcgt accggtaagc acgcccgctt tctgccgacg gttcacagcg
     6541 acgcctgtac cggttgcggt aagtgcgaaa aagtatgcgt gctggaacaa ccggcaatca
     6601 aggtgttacc gctgtcactg gcgaaagggg agttaggtca ccattaccgc ttcggttggc
     6661 tggaggggaa caatggcaaa tcgtaaacgt gacgccgggc gcgaggcgct ggagaaaaaa
     6721 ggctggtggc gcagtcaccg ttggctggtg ttacgtcgcc tttgtcagtt cttcgttctg
     6781 gggatgtttt tgagcggtcc atggtttggt gtgtggatct tgcacggcaa ctacagcagt
     6841 agcctgttat tcgacaccgt tccgctgacc gatccgctga tgacgctgca aagtcttgcc
     6901 agtggtcatc tgcctgccac ggtggcgttg accggtgcgg tgattatcac cgtgctctac
     6961 gccctggcgg gaaagcgatt attttgcagc tgggtctgcc cgctgaaccc gattaccgac
     7021 ttagcgaact ggttacgcag gcgctttgac ctcaatcagt ctgcgacgat ccctcgccac
     7081 attcgctacg tgctgttggt ggtcattctg gtgggatcag ccttaactgg cacgctcatt
     7141 tgggaatgga tcaaccccgt ttctctgatg gggcgtagcc tggtaatggg cttcggcagc
     7201 ggcgcgctgc ttattctcgc gctgttttta tttgatctac tggtcgttga acacggctgg
     7261 tgcgggcaca tttgtccggt tggcgcgctg tatggcgtgc tgggtagcaa aggtgtgatt
     7321 accgttgcgg ccaccgatcg gcagaaatgt aaccgctgta tggattgttt tcatgtttgc
     7381 ccggaaccgc atgtgctacg tgccccggtg ctggatgaac aaagcccggt gcaggtcacc
     7441 agccgcgatt gcatgacgtg tggtcgctgc gtggatgtct gttctgagga tgtatttaca
     7501 ataactacac gatggagttc gggagcgaaa tcatgaaaag ccatgacctg aagaaagcgc
     7561 tgtgtcaatg gacggcgatg ctggccctgg tggtaagcgg cgcggtttgg gcggctaatg
     7621 gcgtcgattt tagccagtcg ccggaagtat ccgggacgca ggaaggggcc attcggatgc
     7681 caaaagagca ggatcggatg ccgctgaact atgtgaatca gccgccaatg atcccgcata
     7741 gcgttgaagg ttatcaggta acgaccaata ccaaccgctg cttgcagtgc cacggtgtcg
     7801 aaagctatcg caccactggc gcgccgcgta tcagtcctac tcactttatg gacagcgacg
     7861 gcaaagtggg cgcggaagtg gctccgcgtc gttatttctg tctgcaatgt cacgtaccgc
     7921 aggccgatac cgcgccaatc gtggggaata cctttacccc atcaaaaggt tacgggaaat
     7981 aagaggttat tatgggaaat tctgaccgta agcctggtct gattaagcgc ctgtggaaat
     8041 ggtggcgtac ccccagccgt ctggcgctgg ggacgctgct gttgagcggt tttgttggcg
     8101 gcatcgtctt ctggggtggc tttaacaccg ggatggaaaa agccaatacc gaagagttct
     8161 gcattagctg ccacgaaatg cgcaacacgg tgtatcagga atacatggat tccgtgcact
     8221 acaacaaccg tagcggcgtc cgtgcgacct gtccggattg tcacgttccg cacgagtttg
     8281 tgccgaagat gatacgcaag ctcaaagcaa gtaaagagct gtatggtaaa atttttggcg
     8341 ttattgacac gccgcagaaa tttgaagctc atcgtctgac gatggcacag aatgagtggc
     8401 ggcgcatgaa ggacaataac tcgcaggagt gccgtaactg tcacaacttc gagtatatgg
     8461 atacaaccgc ccagaaatcg gttgccgcga agatgcatga ccaggcggtg aaagatgggc
     8521 aaacctgtat tgattgccat aaagggatag cgcacaagct gcccgatatg cgtgaagtcg
     8581 agccaggttt ttaacagggt tattgcgtgg gtatgcttga agccagagag ttactttgtg
     8641 agcgggatga acgaacctta tttagtggct tgtcatttac gctgaacgca ggagagtggg
     8701 tacaaatcac cggtagcaac ggcgcgggga agacaacgct tctccgtttg ctgactgggt
     8761 tgtctcgccc tgacgctggc gaggttctct ggcaagggca gcccttgcat caggtacgcg
     8821 acagctacca tcaaaacctg ttatggatag gccatcagcc ggggatcaaa acccggctga
     8881 cggcgttaga aaatctgcac ttttatcatc gcgatggcga tgccgcacaa tgtctggaag
     8941 ccctggcgca ggccgggctt gccggattcg aagatattcc ggtaaatcaa ctttctgccg
     9001 ggcaacaacg ccgcgtcgct ttagcgcgtc tgtggctgac ccgtgccacg ttatggatcc
     9061 tcgacgagcc ttttaccgcg attgacgtta acggcgtcga tcgtctgacc cagcgtatgg
     9121 cgcagcatac ggagcagggg gggattgtga ttctgactac ccaccagccg ctcaacgttg
     9181 ctgaaagtaa aattcgccgc atttcactga cgcaaacggg ggccgcatga tgttctggcg
     9241 cattttccgt cttgagctgc gcgtagcgtt tcgccatagc gccgaaatcg ccaacccgct
     9301 gtggttcttc ctgattgtaa ttaccctttt tccgctcagt atcggtccgg agccgcaact
     9361 gctggcgcgt attgcaccgg gcattatctg ggttgctgcg ctgctttcat ccttgctggc
     9421 gctggaacga ctgttccgtg acgatttgca ggacggcagt cttgaacaat tgatgttgtt
     9481 gccgttaccc ttgcccgccg ttgtgctggc gaaggtgatg gcgcactgga tggtaaccgg
     9541 tctgccgtta ctcatccttt cgccactggt agcaatgcta ctgggaatgg atgtttatgg
     9601 ctggcaagtg atggcgctga cgctgctgct gggaacgcct acgcttggct ttctcggtgc
     9661 accgggtgtg gcgttgacag tgggacttaa gcgcggtggt gtgctgctca gcatactggt
     9721 gttaccgctg actatcccat tactcatctt tgccaccgcc gcgatggacg cggcttccat
     9781 gcatttgccc gttgacgggt atctggcaat tttaggcgcg ttgctggcag gcaccgcgac
     9841 attaagtcct tttgcgacgg cggcagcgtt acgaatcagc attcaataac gctggattac
     9901 tgatttttgt tcgagtctgg tatcgaaact atgtggaaaa cactgcatca actggcgatc
     9961 cctccacggc tgtatcaaat ctgtggctgg tttataccgt ggctggcaat tgccagtgtg
    10021 gtcgtgctta ccgtcggctg gatctgggga ttcggctttg ctccggctga ttatcagcag
    10081 ggaaatagct accgcattat ctacttgcat gtgcctgcgg cgatctggtc gatgggcatt
    10141 tatgcatcaa tggcggtggc ggcttttatt ggccttgtct ggcagatgaa aatggccaac
    10201 ctggcggtgg cggcgatggc ccccattggt gccgtgttta cctttattgc cctggttacc
    10261 ggctctgcat ggggaaaacc gatgtggggc acctggtggg tatgggatgc acgtctgact
    10321 tctgaactgg tgctgctgtt tttgtatgtg ggtgtgattg ccctgtggca cgccttcgac
    10381 gaccgccgtc tggcgggccg tgcggcaggt atcctggtgc tgattggcgt ggtgaatctg
    10441 ccgattattc attactccgt ggagtggtgg aacaccctgc atcagggatc aacgcggatg
    10501 cagcaaagta tcgatccggc gatgcgttcg ccgctgcgct ggtcgatttt tggcttcctg
    10561 ctgctgtctg ccacgctgac gctgatgcgg atgcgtaatt tgattttgct gatggaaaaa
    10621 cgccgtccgt gggtgagtga actgatactg aaaagaggcc gtaaatgacc cctgcatttg
    10681 cttcctggaa tgaatttttc gcaatgggcg gttacgcctt ttttgtctgg ctggcggtgg
    10741 tgatgaccgt tattccgctg gtggttttgg tcgtgcactc ggtgatgcag catcgcgcaa
    10801 ttctgcgtgg tgtggcgcaa cagcgggcgc gtgaggcgcg tttacgtgct gcgcaacagc
    10861 aggaggctgc atgaatattc gccgtaaaaa ccgcttgtgg attgcctgtg ccgtgttggc
    10921 agggctggcg ctgactatcg gtctggtgct atatgcgctg cgctcgaata tcgatctctt
    10981 ttatacgccg ggggaaattc tctacggcaa gcgtgaaact cagcaaatgc cggaagtcgg
    11041 gcagcgtctg cgcgtcggcg ggatggtgat gccgggtagc gtgcagcgcg atcccaattc
    11101 gctaaaagta actttcacca tttacgacgc tgaaggttca gtagatgtct cttacgaagg
    11161 cattttgccg gatctgttcc gtgaagggca gggcgttgtg gtgcagggcg aactggaaaa
    11221 aggcaatcat atcctcgcaa aagaagtgct ggcgaaacac gacgaaaact acacgccgcc
    11281 agaagttgag aaagcgatgg aagccaatca ccgtcgcccg gcgagtgttt ataaggaccc
    11341 agcatcatga tgccggaaat tggtaacggg ctgctgtgtc tggcgctagg aattgcgctg
    11401 ctgctgtccg tgtatccgct atggggcgta gcgcgcggag atgcgcgcat gatggcgtct
    11461 tcccgcttgt ttgcctggct gctgtttatg tctgtggctg ccgcatttct ggtactggtc
    11521 aatgccttcg tggtcaacga cttcaccgtc acctatgttg ccagcaactc caatacccag
    11581 cttccggtgt ggtatcgcgt ggcggctacc tggggcgcgc atgaaggctc gctcctgctg
    11641 tgggtgctgc tgatgagcgg ctggaccttt gcggtagcga tttttagtca gcgtattccg
    11701 ctggatattg tggcccgcgt actggcgata atggggatgg tcagcgtcgg ctttttgctg
    11761 ttcattctct ttacctctaa cccgttctca cgcacgttgc cgaacttccc gattgaaggg
    11821 cgcgatctta acccgctgtt acaggatccg gggctgatct tccatccgcc tctgctctat
    11881 atggggtacg tggggttctc ggtggcgttt gcttttgcca ttgcttcttt gttgagcggg
    11941 cgtctggaca gcacttatgc gcgttttact cgtccgtgga cgctggcggc gtggatcttc
    12001 ttgacgctcg gcattgtgct cggttccgca tgggcctatt acgaactcgg ctggggcggc
    12061 tggtggttct gggatccggt agaaaacgcc tcgtttatgc cgtggctggt ggggactgcg
    12121 ctgatgcact cactggcggt cactgaacaa cgcgccagct tcaaagcgtg gacattactg
    12181 ctggcaatca gtgccttctc gttgtgtctg ctggggactt tcctggtgcg ttccggcgtg
    12241 ctggtatcgg tacacgcgtt tgcctcagat ccggcacgcg gtatgtttat cctcgccttt
    12301 atggtactgg tgattggtgg ttcgctgctg ctgtttgccg cgcgtggaca caaagttcgc
    12361 tcacgcgtaa acaatgcgct gtggtcgcgg gaatctctgc tgttagcgaa caacgttttg
    12421 ctggtcgccg cgatgctggt ggtattactg gggacgctgc tgccgctggt gcacaagcaa
    12481 ctgggactgg gcagtatttc gattggcgaa ccgttcttca acaccatgtt tacctggctg
    12541 atggtgccgt ttgcgctgct gcttggtgtc ggtcctctgg tgcgctgggg gcgcgatcgc
    12601 ccacgtaaaa tccgcaattt attgattatc gccttcatct caacgctggt gctgtcgctg
    12661 ctgctgccgt ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg cctggcaatg
    12721 gcctgctgga ttgctgtgct ggcaattgcg gaagctgcgc tgcgtatttc acgtggcacg
    12781 aaaaccacct tcagttattg gggaatggtg gcggctcacc ttgggctggc agtgacaatt
    12841 gttggcattg cctttagtca gaactatagc gttgagcgtg atgtgcgcat gaagtccggc
    12901 gatagcgtcg atattcatga atatcgcttc accttccgtg atgtcaaaga ggtgactggc
    12961 ccgaactggc gtggcggtgt ggcgactatc ggcgtaacgc gagagggtaa accggaaaca
    13021 gtgctgtatg cggaaaaacg ttattacaac actgccgggt cgatgatgac cgaagcggcg
    13081 attgacggtg gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct ggaaaacggc
    13141 gcgtgggctg tgcgtcttta ctacaaacca tttgttcgct ggatttgggc gggcgggctg
    13201 atgatggcgt tgggcggact gctgtgtctg tttgatcctc gctatcgtaa gcgcgtgagt
    13261 ccgcaaaaaa ctgcgccgga ggctgtatga agcgcaaagt attgttaatt ccgttgatta
    13321 tcttcctggc gattgccgcg gcgctgctgt ggcagttggc gcgtaatgcc gaaggggatg
    13381 atccgaccaa tctggaatcg gcgctcattg gcaagcctgt gccgaagttt cgtctcgaat
    13441 cactggacaa tccggggcag ttttatcagg cggatgtgct gactcagggc aaaccagtgc
    13501 tgcttaacgt ctgggcgacc tggtgtccga cctgccgtgc ggaacatcaa tatctgaatc
    13561 agctttctgc gcagggcatc cgagtggtcg gcatgaacta taaagacgat cgccagaagg
    13621 caatcagctg gctgaaagag ctgggcaatc cttacgcgct aagcctgttt gatggcgacg
    13681 gcatgttagg gctggatctc ggtgtctatg gcgcgccaga aaccttcctt attgacggca
    13741 acggcatcat tcgctatcgc catgcgggcg atctcaatcc tcgcgtctgg gaagaagaga
    13801 tcaagccgct gtgggagaaa tacagtaagg aggccgcaca atgaggtttt tattgggcgt
    13861 gctgatgctg atgatctccg gctcagcgct ggcgaccatc gacgtgttgc agtttaaaga
    13921 tgaagcacag gaacaacagt tccgtcagct cactgaagaa ctgcgctgcc cgaaatgcca
    13981 gaataacagc attgccgatt ccaactcgat gattgccacc gacctgcgcc agaaagtgta
    14041 tgaactgatg caggaaggta aaagtaagaa agagattgtc gattatatgg tggcgcgtta
    14101 cggcaacttc gtcacttacg atccgccgtt aacgccgctg accgtgctgc tgtgggtgct
    14161 gccagtagtg gctattggca ttggcggttg ggtcatttac gcccgctcgc ggcgtcgggt
    14221 acgcgtggtg ccggaagcgt ttcctgaaca aagcgtgccg gaaggtaagc gtgccggata
    14281 tgtcgcttat ttgccgggca ttgtggtggc gttaattgtg gctggcgtca gctactacca
    14341 gaccggaaat tatcaacagg tgaaaatctg gcagcaggcc acggcacagg ctccggcgtt
    14401 gctggacagg gcgctggatc cgaaagccga tccgctcaac gaagaagaga tgtcgcgtct
    14461 tgcgctgggg atgcgtactc aactgcaaaa aaatccggga gatatagaag gctggattat
    14521 gttgggccgc gttggcatgg cgctgggtaa cgctagtatc gccaccgatg catacgctac
    14581 tgcgtatcgc ctcgatccga aaaacagcga tgccgcactg ggttatgctg aagcgttgac
    14641 acgttcatct gatcccaacg acaaccgcct cggcggcgaa ctgctgcgcc agttggtgag
    14701 aagcgaccac agcaatatcc gtgtgttaag catgtatgcg tttaatgcct ttgagcagca
    14761 gcgatttggc gaagccgttg ccgcgtggga gatgatgttg aaactcttac ctgccaacga
    14821 tactcgccgt gcggtgattg aacgtagtat cgcgcaggcg atgcaacatt tgtcgccgca
    14881 ggagagtaaa taaaaagagg cccgatggtg atgccatcgg gctattttta ttgtgccccg
    14941 cgttgttgca ggaacagaat ggtggctgcc acgcgtgagc ggacattgag tttgcgcagc
    15001 agattgcgaa tatgtacttt tactgtctgc tctgaaatat tcaacaccga ggcaatctgt
    15061 ttatttgaca gcccctgtgc cagctcgtgc agaacatcca gctcgcgctc cgtcagcacg
    15121 ctgaagggat cttcttccgc gccaaacatt tcacgttcac gtaagtactg attgacgcgt
    15181 tcgctaaaga ctttgctgcc tttcgctcca gcacgaattg cttccagcaa tacttccggg
    15241 tcgctgtctt tcaacagata accgtctgcg cctgcgtcta tcagtgcaaa gacatcgctg
    15301 gaggcatctg atacggtcag gataataatt tgcgcggtaa cgccatccct gcgcaaggca
    15361 ttgagggtat ccaggccact catacctttc atattgagat ccagcaagat cacgtcgata
    15421 tccagtctat tcgccagatc gatcgcgctc gcgccgtcgc ccgcttcggc gaccacttca
    15481 aagccaggat caagctccag taactgacga acaccgcgtc gcataagtgg atgatcatcc
    15541 acaatcatca cctgaaaagg cgttgcttca ggcatagtag tctcctgagg ttttattaga
    15601 atcattatgt ttgagcgtag cgttggtaac gctaccaggc gcgtgaatgt taccccattt
    15661 tatgtatgga cttgtagtga aaactacaac cttgtcgttc gctttggcat cattttcatt
    15721 ataaagacgg aattttgcat cagcttctca tcaagaaaaa caactaaaaa atcaatcaca
    15781 atacttgtgc atttctttaa acagttttta aaaactgact ccgggtatgg agctatgggt
    15841 attttccgta cccaatgctt ttaacagcaa ttaatttcat aggatgaaag ctcaattcat
    15901 caatctggtt ctgtttctct ttgtcgttcc gcaatatctg ttctggtggc tacagcgtta
    15961 tattcaccca tagcattggc atcaacagtt gagtatggtg agacagttga tggtgttgtc
    16021 ctggaaaaag atatccagct ggtttatggg accgccaata atacgaaaat caatcctggc
    16081 ggagaacagc atataaaaga atttggtgta agtagtaata ctgaaattaa aggagggtat
    16141 cagtacattg aaatgaatgg taccgcagaa tactcagtat taaatgacgg ttatcaaatt
    16201 gttcaaatgg gtggcgcggc aaaccagact accctcaata atggtgtgct acaggtttat
    16261 ggcgcagcga atgatcccac gattaaaggc gggcgcttaa tcgttgaaaa agatgggatt
    16321 accgtccttg ccgctatcga aaagggagga ttactggagg ttaaagaggg gggattagcg
    16381 attgcggtag atcagaaagc aggcggtgct attaaagcaa gcacgcgggt catggaggca
    16441 ttcggaacaa accgtctcgg tcagttcgaa atcaagaatg gtattgctaa caatatgctg
    16501 ttggaaaacg gcggaagttt gcgagttgaa gaaaatgact tcgcttataa tactactgta
    16561 gatagtggcg gcttactgga ggttatggat ggcgggactg taactggcgt tgataaaaaa
    16621 gcaggcggaa aattaattgt ctcaacgaat gcgctggaag tgagtggtac aaacagtaaa
    16681 ggccaattta gtataaaaga tggtgtgtca aaaaattatg aactggatga tggttccggg
    16741 ctcattgtta tggaggacac gcaggccatt gatactatcc ttgatgagca tgccactatg
    16801 caatcgctgg gaaaggatac tggtacgaaa gtgcaggcaa atgcggtata tgatctcggt
    16861 cgatcagatc agaatggaag tatcacgtat tcctcaaaag ccatctctga aaatatggtt
    16921 atcaacaatg gccgcgctaa cgtctgggct ggcacaatgg ttaacgtttc agtcagaggg
    16981 aatgatggca ttcttgaggt catgaagccg caaataaatt atgcacccgc aatgttggtg
    17041 ggtaaggtag tggtttctga gggcgcttct tttagaacgc atggtgccgt ggataccagc
    17101 aaagcggacg tttcgctcga aaatagcgta tggaccatca ttgccgatat cactacgacg
    17161 aaccaaaaca ccctcctcaa cttagccaac cttgcgatgt ctgacgcaaa tgtgattatg
    17221 atggatgagc cagtgactcg ttcatcagtg acggcaagtg cggaaaattt cattacgttg
    17281 accaccaata ccctgtcggg aaacggcaat ttttatatgc gtaccgatat ggctaatcat
    17341 cagagcgatc agctcaacgt caccggtcag gcaacaggtg atttcaaaat attcgtgacg
    17401 gacaccggtg ccagcccggc agcaggagat agccttacac tggtaacaac gggcggcggt
    17461 gatgctgcat ttacgttggg caatgccgga ggcgttgttg atatcggtac gtatgaatat
    17521 accttgctgg ataatggcaa ccatagctgg agtttggcag agaatcgcgc gcaaattacc
    17581 ccttcaacca ctgatgtgct gaatatggcg gcagcacaac cgctggtatt tgatgcagaa
    17641 ctggacaccg tgcgtgagcg tcttggtagc gtaaaaggcg ttaattacga tacggcgatg
    17701 tggagttcgg caattaacac ccgcaacaac gtgaccactg atgcgggcgc tggctttgag
    17761 caaacattga ccgggctgac gctcggtatc gatagccgtt tctcccgtga agaaaccagc
    17821 acaattcgcg gcttgttctt tggttactct cattctgata ttggttttga tcgcggcggc
    17881 aaaggcaatg tcgatagcta taccctgggg gcttatgccg gttgggagca tcagaacggt
    17941 gcctatgttg atggagtggt gaaagttgac cgttttgcca acaccatcca ttgcaagatg
    18001 agtaatgggg caacagcgtt tggcgattac aatagtaacg gcgcgggtgc tcatgtcgag
    18061 agcgggttcc gttgggttga cggattgtgg agtgttagac cctatctggc ctttaccggc
    18121 tttaccacag atggtcagga ctacacgtta tcaaacggca tgcgcgcgga tgtgggaaat
    18181 acccggatat tacgcgctga agcgggaacg gcggtaagct atcacatgga cctgcaaaac
    18241 ggtacgacgc tggaaccctg actgaaagcc gccgtgcgtc aggaatacgc cgattctaac
    18301 caggtgaaag ttaatgacga tggcaaattt aataatgatg tggctggaac ccgtggcgtt
    18361 tatcaggctg ggataaggtc atcgtttacc ccgacgttaa gcggtcattt gtcagtcagc
    18421 tatggcaatg gcgcaggggt agaatcgccg tggaatactc aggcgggtgt ggtctggacg
    18481 ttctgataac aaaaaataaa caggctgtga tgtgtcacgg tctgtttatc gaattaattg
    18541 cagatataaa aaaaaccaac cgtaaaggtt ggttttttct tgggattttt ggtcggcacg
    18601 agaggatttg aacctccgac ccccgacacc ccatgacggt gcgctaccag gctgcgctac
    18661 gtgccgactc gtggctgcta atactaccgt tttccacacc gattgcaagt aagatatttt
    18721 gctaactgat ttataattaa tcagttagcg ataaaacgct tctcgtctgt cagcacttgc
    18781 aataacaaac ttaactgtgg tttttcatct ttcactcttt caccacgtaa gttgtaagtg
    18841 cggtatttac cgttattgtt cagcaccagc gtctttttcg gggtggtaat tgccagcgta
    18901 tcgttatccg ctgcagtaac ccagtaatga cggcgttgag ggttgaacaa atcctgacct
    18961 tgcgaatatt cgctggcagg tgtgctgaca tgtagcaggc gttgcatcag cgtcgtcatc
    19021 agatcggtat gatcagtcag agcattaata cgctgcgccg gcgtgcctgg ccagtgaatc
    19081 actaatggca cctgcagatg accgtgggac cagtcaaagg tttcttcgtc ttcgctcagc
    19141 ggaatacccc gaccggcagt gataatcaca accgtattgt ccagtttgcc agaatcacgc
    19201 agtgcattga gcacgcggtt gatctggtca tcgacattgc ctgccgcccg gctatatttc
    19261 cgtgcaaatg cctgctgatt gctgtcgtca atgttagtgc cattgaaaga ggcccacgag
    19321 aaccagcggt tatcttcttg tgcgtagcgg cccagccagt tgatccactg cgtggcggtc
    19381 tgctcgtcgg attgggtgcg tacgctcggc atcgagaaat ctgacaacaa tgcctggcga
    19441 tacagcgggc tggtaaagcc atctgatgag aataatccca gctgatagcc ttgctgatta
    19501 agcgcagtaa ttaacgccgc aggggtacgg gtcgacagaa tgccgtccat atagctcggc
    19561 gagatgccat agaacaggcc aaagatgccg ttgtctgtag tgttgccgga gctcatatgg
    19621 cgcgtgaacg aaatattttg ctcagcaaaa cctgccagcg caggcatctg cttctcgaaa
    19681 cgtgagtagt tcaggccatc gacagtaatc aacagcacat tctgcccggt gcccatatcg
    19741 cgatagcgca gttcgcttaa cggatactga acggaaacgg cgtctggatt accttgctca
    19801 ataagacggc gttgatactc ctgcgcatca agcagaccat gcttctcaag aaaacgtcgc
    19861 gccgtcatcg ggtacgaaag cggcaggtta gcgcgctgca tggtgatcgg gcgatagaag
    19921 ttggcatcgg cccagatata caccacatgc gaggcgataa aggcgataaa taagaatgcg
    19981 gccagcgggc gcgcgaagcg ccgacgacgc gtcaggctgc gcagcttttg ccagctccac
    20041 gtcgcaaaca ccagttcaag caataaaata accggcacgc tgatgaacat cagctgccag
    20101 tcgcgcgcca tctcattttc gtctgggttg ataaccagtt gccagacgat gggattaaga
    20161 tggagatgga aacgagtaaa gacttcgcta tcgatcagta atagcgtcat tcccgccgtt
    20221 gccagaatga cggacaaaaa cctcatcagc ctctgggagc cgacgataaa ggtcagcggg
    20281 aagaggatca gcaagtaggt ggcgaacacc aggaagctga aatggccgat aatgcttacg
    20341 taggaataaa tgcgaccagc aagcgttgtc ggccagtcgg cgataaacag gtaacggctg
    20401 ccaatgacga gcgaaagcag aatattgaac agtgcaaacc agtgccccca actgaccatc
    20461 tgggagactt tttcacggta gcgctgacga tgagttacca taacctgtta tctgtttccc
    20521 ttagtgcgct ttgtcttcgt tgatagagga ctgtaaggcg cgggcaaaag agttggcaat
    20581 tgcctggcgt tgggccgggg caatgctggt gttgataagg ttagtgacca tgttgcctaa
    20641 caccatcagg gaaaggtcgg tcggagcctt atgtttttcc agtacgttga gcagctctgc
    20701 gagcaattgt tcaacctgtt catcactgta gcgggaaatt tgtggcataa atcgaaatca
    20761 gttagttgat gaaagggcaa catattaccg tagcaacggt tttttttctg cttttttatg
    20821 cgttaaatca ttctcgttac gctgccttct gtaaaatgac cgccatcttt tattgcgtcg
    20881 cttaaccggc ggtggttgaa taccgcccgg tcttaaagga gagtttatca tgagtctgga
    20941 tatcaaccag attgccctgc accagcttat caagcgtgat gagcagaacc ttgagctggt
    21001 gttgcgcgat tcattgctgg aaccgacaga aaccgtcgtt gagatggtgg cagaactgca
    21061 tcgggtctat agcgccaaaa ataaagctta cggcttgttt agcgaagaga gtgaactggc
    21121 gcagacgctg cgcttacagc gtcagggcga agaggacttt ctggcattta gccgcgcggc
    21181 aaccgggcgc ctgcgtgacg aattagcgaa atatcctttt gctgacggtg gattcgtgct
    21241 gttttgccac taccgttatc tggcggtgga gtatctgctg gtggcagttc tgagcaacct
    21301 gagcagtatg cgcgttaacg aaaatctcga catcaaccca acccattatc ttgatatcaa
    21361 tcatgcggat attgttgcgc gtatcgattt aaccgaatgg gaaaccaatc cagagtccac
    21421 ccgctatctc actttcctga aagggcgagt agggcgcaaa gtggcggatt tctttatgga
    21481 tttcctcggg gccagcgaag gattgaacgc gaaagcgcaa aaccgtgggc tgttgcaagc
    21541 ggttgatgat ttcactgccg aagcacaact ggataaagca gagcgacaga acgtgcgcca
    21601 gcaggtttat agctactgta atgagcagct gcaggcaggc gaagagatcg aactggaatc
    21661 gctgtcgaaa gagctggcag gcgtaagcga agtgagcttc acggagtttg cggctgaaaa
    21721 aggctacgaa ctggaagaga gcttcccggc agaccgtagc acactgcgtc agttgacgaa
    21781 gtttgccggt agcggcggtg gcttgactat taactttgat gcgatgctgt taggtgaacg
    21841 catcttctgg gatccagcaa ccgatacctt gaccattaaa ggcacgccgc cgaatttgcg
    21901 cgaccaattg cagcgccgga cgtctggcgg caattaatcg gttttgtgtt ctgaatgcca
    21961 gatgcggcgt gaacgcctta tctggcctgc aaaaaatagc aataccgtaa tcaggcataa
    22021 tctgcagata caaaaaaccc cgccggagcg gggtttttta caacttattc agcaattaag
    22081 cgcgacgaag tcgatgtgct gcagcttcgg tttgtacggg tgacgctgta cgtcctgagc
    22141 tttaactttg atttctttac cgtcaacaac gatggtcaga acttcgctgt agaattcagc
    22201 tttagcttgc atgttcatga ctttgtcgtg atccagctcg atagccagcg gcgcttcttt
    22261 gccaccgtag atgattgccg ggaacttgtt agcggcacgc aggcggcggc tcgcaccctt
    22321 accctgctct ttacgtactt ctgcgttgat agtaaacatt tctttctctc ttaaattaat
    22381 cctgctacag gcgacccagc aacaggtaag tgatctgctt tgcgtatgca aaagcgggcg
    22441 agattctata ctcaatcgct ttatacatca atcaaaacta ccattaaccg cgtaattcgt
    22501 gcgcccgacg aaaacgacct tcgtaatcga acactttttc acgcacttgc cagtactgac
    22561 ctttcatgcg ggcaacgaca aaatccgggt gtcgcaataa ggcttgctgg gcgaggatat
    22621 cggcggcggt gatccagcgc agagggatgc ccggtgtgcg cgtatgcggg cggataaaaa
    22681 gctgctcgaa ggcagttcgc tgggcgggcg tttgcagacg gaaacgctca ctcacatccg
    22741 cgccgtcttc atcgtaatag gtgattttca accattcgcc tttctcgtcg tgctcatgct
    22801 gcaaagacat gccgctacag cgtaatacca gcgcatcttt cagtcgtagc gccgctttta
    22861 acatatcatc cggatcaacc agtaccgtgt cacattcgcg gcagcggcga gcggcaatat
    22921 cattttctgc gttacattgc gggcaatttt taaaacggaa acggaagtca cactgttcgc
    22981 gatgaccgtc gtcatcttca aaccatccct gacagcggcg accaaagtgt tcaatcaatg
    23041 tcccgtcggc ggtcgttttc ccccaaaagg tgttggcaaa accgcaggca gggcagaaaa
    23101 cctgtaccgg aacgttgtca cttttgcctt tcggtgtacc aacttccggc gcgtagagat
    23161 cgtgaggatt acccgcataa tcaagaatta aacaatcagt cttgcccgga gcgagacgca
    23221 gaccgcgccc gacaatttgt tggtaaagac tcactgattc ggtagggcgc agaatggcga
    23281 taagatcgac gtgcggggcg tcaaatccgg tggtcagtac tgcgacgttg accagatagc
    23341 gaaaacgctg ggctttaaaa tcttcaatta acacatcgcg ctcagcgccg ggggtgtcgc
    23401 cagtaatcag tgcagcatct tcggcaggca gtaatcccac aatctctttt gcgtgttcaa
    23461 cggtggcggc gaaaatcatt acccctttgc gcttttcggc gaactccatg atctggctga
    23521 tgatatgtgg cgtaatgcgt tgctgttttt tcagctcacg gttgagatca gcttcgctga
    23581 acagcccgtt actctgtgcc tgcaagcggc tgaaatcgta ttgcactact ggcatatcca
    23641 gtcgttctgg cggcgtcaga tagccgtgtt taatcatata acgcagcggc agctcataaa
    23701 tgcagtcacg gaaaagggct ttctcatcgc cgcgtaccat gccgtgataa tggaactggt
    23761 agatccagcc tttgcccaat cgaaaaggcg tggcagtcag ccccagcagg cgtaagtggg
    23821 gattcacttt agtcaggtga gtgaggattt gctgatactg gctctcttca tcgtcaccaa
    23881 tacggtggca ttcatcgaca atcaacagcg aaaattcacc ctgaaaggca tcaagattac
    23941 gagcgaccga ctgcacgctg ccaaatacca ctttaccgtg gctctctttg cgctttaacc
    24001 cagcggcaaa aatatcggct tccagcccca gcgcctgata ctttgcatgg ttttgcgcca
    24061 ccagttcttt gacgtgtgcc agcaccagaa cacgaccacg cgccagccgt gccagttcgg
    24121 caatcaccag gcttttacct gcgccggtgg gcagcacgat aacggcaggg gttttatgac
    24181 gacgaaaatg gttgagcgtg gcatccacgg cttcttgctg atatgggcgg agtgtaaaaa
    24241 tcatggtctc gcgtaggtag attattctgc taatagtatg ccatggatct ttttcccttg
    24301 agggatgtta ttagaccgct atactgggca gacaacagtt cagattttcg ggtcaatgcc
    24361 cgacctccgt ttttacaggc aaaatcacac acatgcgact tgataaattt atcgcacagc
    24421 aactcggcgt tagccgtgct attgccgggc gtgaaatccg cggcaatcgt gtcaccgtcg
    24481 atggcgaaat tgttcgtaat gcagcgttca aactgcttcc tgaacatgat gtcgcttacg
    24541 atggcaaccc gctggcgcag caacatggtc cacgttactt catgctcaat aagcctcagg
    24601 gctatgtttg ctccacggac gaccctgatc acccaacggt gctctatttt ctggatgaac
    24661 cggtagcctg gaaactgcac gccgcaggac gtctggatat cgataccacc ggtctggtgc
    24721 tgatgactga tgatggtcag tggtcgcacc gcattacttc tccgcgccat cattgcgaga
    24781 agacctatct ggtgacactg gaatcacctg tagctgacga tacggcagag caatttgcta
    24841 aaggcgtgca gctgcataac gaaaaagatc tcactaagcc tgcggtgctg gaagtgatta
    24901 ccccaacgca ggttcgtctg accatcagcg aagggcgtta tcatcaggtg aaacgcatgt
    24961 tcgccgctgt gggtaaccat gtggttgagc tgcatcgtga acgtattggc ggtattacgc
    25021 tggatgctga tttagccccc ggtgaatatc gtccgttaac tgaagaagaa attgccagcg
    25081 tcgtctaact tttccatgac tttcaggagc ccgttgtgac cacccgacag cattcgtcgt
    25141 ttgctattgt ttttatcctt ggcctgctgg ccatgttgat gccgctgtcg attgatatgt
    25201 atctgcccgc gctaccggta atttcagcgc agtttggcgt acctgcgggc agtacgcaga
    25261 tgaccctcag tacttatatt ctgggctttg cgttggggca gttaatctac gggccgatgg
    25321 cagacagctt tgggcgtaag ccggtggttc ttggcggtac gctggtgttt gccgccgccg
    25381 cggtggcgtg tgcgttggca caaaccatcg atcagctgat tgtgatgcgt ttcttccacg
    25441 ggctggcggc ggctgcggcc agcgtggtta tcaacgcttt gatgcgtgat atctacccga
    25501 aagaagagtt ctcgcggatg atgtcgtttg tcatgctggt gacgaccatt gcaccgctga
    25561 tggcaccgat agttggcggc tgggtgctgg tgtggctgag ctggcattac atcttctgga
    25621 tcctcgccgt ggcggcgatt ctggcttcgg caatgatctt ctttctgatc aaagaaacct
    25681 taccaccgga gcgtcgccag ccttttcaca ttcgtaccac tattggtaac tttgcggcgc
    25741 tgttccgtca taagcgtgtc ctgagttata tgcttgccag tggtttcagc tttgccggga
    25801 tgttctcgtt cttaagcgcc ggaccgtttg tctatattga aattaaccac gtcgcgccgg
    25861 aaaactttgg ttattacttt gcactaaaca ttgttttcct gttcgtgatg accattttta
    25921 acagccgctt cgtccgccgt attggcgcgt taaatatgtt ccgctcgggg ttgtggatac
    25981 agtttattat ggcggcgtgg atggtcatca gtgcgctgct ggggctggga ttctggtcgc
    26041 tggtggttgg cgttgcggcg tttgtgggct gcgtgtcgat ggtgtcatcc aatgcgatgg
    26101 cggtcattct tgatgagttt ccccatatgg cgggaacggc atcttcgctg gcaggaacct
    26161 tccgttttgg cataggggca attgttggcg cattgctttc tcttgcgacc tttaactctg
    26221 catggccgat gatttggtca attgcattct gcgcaagcag ctccattctc ttctgtctgt
    26281 acgccagtcg accgaaaaaa cggtgatcta ttgcacaacg aggaagctaa aaggcttcct
    26341 ttgttgatgc atgtcaacca caaatctatc attcccccga tatatgttta ttttatgtaa
    26401 aatcaattta tgtaaaaagt cacatcattg tagttaaaaa ggttgagtta gatcgcagaa
    26461 acgggtacat atagccccgc aaacgtgacc acgcccgcag atattactta aatcagagcc
    26521 atagaggcca cgcaggcgag gcatcaatct ttacgatctg tataaagacg gattgttgat
    26581 gatgtgttaa aattgatgta aacaaattgt gaagtgaatg tgcttccggg gaaaataagt
    26641 gacttcatta caactctcaa tcgtccatcg actgccgcag aactatcgct ggtctgctgg
    26701 tttcgcaggt tcgaaggttg aaccgattcc gcaaaatgga ccgtgcggtg acaacagcct
    26761 ggtggcgctt aaattgctta gcccggatgg tgataatgca tggtcggtga tgtataaact
    26821 aagccaggcg ttaagcgaca tcgaagttcc atgttcggtg ctggagtgtg aaggtgagcc
    26881 gtgcctgttt gtaaatcgcc aggacgagtt tgctgcaaca tgccgattga aaaattttgg
    26941 tgtggcaatt gccgaaccct tttcaaacta caatcctttt taagcgtcag ctcaatgcca
    27001 gtagctgacg cgtatactcc tgctgcggtg tggcaaacac gcgcgcgcac ggtccttgtt
    27061 ccactacttc cccttgtcgc agtacgataa cctgatgaca taacgcgcgg acaacgtgca
    27121 aatcgtggct gataaacaaa taggccagtt gatgcttttg ttgcaatgat ttcaatagcg
    27181 tcaatatttg cgcctgtacc gttttgtcga gtgatgatgt cggttcatca agtatgatca
    27241 gcgagggctt aagaattaat gccctggcaa tcgcaatacg ttgtcgctga ccaccagaga
    27301 actccgccgg ataacggtgg cgtgtttcag gatctaatcc cacttcatgc atcacggcta
    27361 tcacttgttg ttcgcgttgt gcggcagaaa gcgtcggctg gtgaacccgt aagccttcct
    27421 caataatctg caaaacgttg agtcgtgggt tgagcgagga gtttggatcc tgaaatacca
    27481 cctgaatgcg atgacgaata ggtaacagct ggcggcgatt taaattttgc agtggctgac
    27541 cgtcaaagat gatgctgccc tgagaattaa tcagtcgcag cagcgccagt cccgtcgtac
    27601 ttttcccgga accggactcg cccactaaac ccagtgtttc acccgctcgt agcgtaaaac
    27661 tgatgttttt caccaccaca ttatgatcca caatgcgctt caaaatccct ttgcgaatgg
    27721 ggaaggcaac ctgaagctgt tcaacatcca gcaacgttga ggcaggttct ggcaacggca
    27781 ctggatcgcc tgacggttca ctgttgagta gcttttgtgt gtaaggatga gtgggtgagg
    27841 caaatagcgt agcggcgtaa ttttgctcga cacagcgacc gttttgcatt accgccacgc
    27901 ggtgggccag ttttctgaca atgctgaggt tatgagtaat aaacagcatg cccatattca
    27961 gctcgccttg cagttcgcgc aacagctgta aaatctgcgc ctggacagag acgtccagtg
    28021 cggtggtcgg ttcatcggca attaataatt ccggtcgcgt taacagcgcc atcgcaatca
    28081 tcacccgctg gcgttcgccg ccggagagct gatgcggata atctgtcagc cgttttgccg
    28141 cctggcggat accaacgcga tcaaggcagt taagaatttc gcctcgagcc gcttcccgac
    28201 gcatcccgcg gtggagtgaa agcacttcat aaagctgttt ttccagggta tgcaatggat
    28261 ttaacgacac catcggctct tgaaaaatca tggcgatctt attaccgcgt acaccgcgca
    28321 acgtttgatc gctggcatga agcagcgatt cgccacgaaa acgaatatcg ccggagagat
    28381 attcaaccgg cggggaaggg agcaggcgta aaattgacag cgcggtaacg cttttgcctg
    28441 aacctgactc acccaccagc gccagcgttt cgccagcctc aatctgtagt gaaacatcat
    28501 tgactactgt acgtacggtt tgctgatggc gaaaacccac cgacaaatct tcaatcgcta
    28561 acagagtttg cgtcatgcta caccgcctta ttaggatcaa atgcgtcgcg gacggcttca
    28621 ccaataaaga tcagcaaaga caataatatc gccaccgaca agaaggcggt gatcccaagc
    28681 cacggagcct gaaggttatt tttcccttgt aacagcagtt caccgagtga cggtgaaccg
    28741 agcggcagac cgaagccgag gaaatcgagt gaggtcaggg tggttatcga actacataaa
    28801 ataaacggta aaaaggtgag ggtcgcgacc atggcattag gcaacatatg acgcaggatg
    28861 atactgcgat cgctgacgcc aagcgcctgc gccgcacgaa tgtagtcgaa attacgggta
    28921 cgtaaaaact ccgcccgcac cacgccgacc agactcatcc agccaaacaa gacagtaatt
    28981 gccagcagcc accagaagtt aggctgtacg acgctggaaa gtaaaataat caaaaacagc
    29041 gtcggcatcc ccgaccatac ttcaataaag cgttgccccc agagatcgac tttaccgccg
    29101 taatagcctt gtagcgcccc cgccagcacg cccatcacgc tggaacagag agtcagcatc
    29161 aggccaaaca gaaccgagat ccgcgtgcca tagagaatac gtgccagcac atcgccgccg
    29221 ttggcatccg ttcccagcca gttttgccgg gagggtggag aagggaaggg cttatcggta
    29281 gcaaagttga tactggtagc accaaagcga atcggtgccc acagtaccca gccgttattt
    29341 tccagccgtt gtttcagcca cgggtcctga taatcagcct gacttgccag cgggccgcca
    29401 aaatcgcttt cgctgtagtt tttcaacaac gggaaatacc aactgccgtc ataacgtacc
    29461 agcaacggtt tatcgttggc gataagttca gaacacaaac tcaaaccaaa caaaacgagg
    29521 aaaatccata acgaccagta gccgcgacgg ttatggcgaa aacgcgccca acgggcctga
    29581 ttgacggggc tgagtcgcga cattaacgtc cctcaaaatc tatacgcgga tcgaccagcg
    29641 tatagctgat atcactgaca atattcagca gcaggccaat cagggtgaaa atatacaagg
    29701 taccaaacat tacaggataa tcgcgcgaga cggtcgcttc gtagcccagt aagcccagac
    29761 cattgagtga aaacatcacc tcaatcagca gcgagccggt aaaaaacatg ctgataaacg
    29821 tcgccggaaa accggcaatc accagcagca tggcgttgcg gaacacatgt ttccagagaa
    29881 tatttttttc acttacccct ttcgcacgcg cggtcaccac gtattgcttg cgcacttcat
    29941 caaggaatga gttttttgtc agcatggtca gcgccgcaaa gccaccaatc actgtcgcca
    30001 gcaccggcag cgtgatatgc cacagataat cggtgatttt ctgataccac ggcagcgaat
    30061 caaagttagc ggaaaccagg ccgcgtagag ggaacaggtc gaaatagctg ccgcccgcga
    30121 agaagacaat cagcaggatg gcaaacaaaa aggccggaat ggcgtagccg atgatgataa
    30181 atgcgctact ccagacgtca aagcggctcc cattataaac agctttgcga atgcctaacg
    30241 gaatcgacac cagatagata atcagcgtgc tccacaatcc gagggtgatg gaaaccggca
    30301 gactgtcttt aatcagcgtc agcaccgagg cgctgcgaaa caggctatcg ccaaaatcaa
    30361 agcggatgta gtcccagagc attttgaagt aacgttcgtg gatcggctta tcaaaaccgt
    30421 agcgatgagt gatctcagcg atcacttctg gatctaatcc gcgtccgcca cggtacttac
    30481 tgtcgctgat attgccgaca cccgtttgcg catggctggc acgaacacct tcaccgcctg
    30541 cgccgggtaa tactccggca ttaccaaact caatggcggc gatggcctgg tcgaccggac
    30601 cgccaggcgc aatttgcacg atgaaaaagt tgatggtgat aatcgcccat aatgttggga
    30661 tcaccagcaa cagacggcga atcaggtaag cgcccatcta ctctccctgt ttgctggcgg
    30721 acggcagttt ggccgcttta ttgacgtcat accaccaggt atcgataccg aggctataga
    30781 tggggcgcac ggccggctgg gagaatttat cccaccaggc gagacggtct tccgccatgt
    30841 accacattgg cagcatgtaa taattccacg ttaatacgcg atccagtgct cgccccaacg
    30901 gcagtaattt ttctttattt ccctgcgcgg caataatttg gttgatcagc gagtcgataa
    30961 ccgggctttg cacgccgggg gcattataag tggaattgat atattccgat gaccaggaaa
    31021 tctgtaaatc ggaactgggc cacggcatcg cccgccatag gcgcggcatc atgtcatagt
    31081 cgcgactgcg catgcggtta gtgatttgcg agttatccac cttgcgaatg tccatattga
    31141 tacccagccg ttgcaggctg tgctggaacg gcaataccca ctgactattg ctgcttgcgg
    31201 gaagcaataa ttcaaagctg agcggctgac ccgttgtggc attaacgcgt tgctgaccct
    31261 tcagcaccca gcccgcttcg ttgagaagtt tgtcggcttt taacaggttg tcacgatcgt
    31321 agccatcgcc tttagatacc ggcggctggt agatttgtgt gaagacttct ggcggtagat
    31381 cttttttcat tggtgccaga agcaccagct ccgcggcgtc ggggtaattt ctggccgcgt
    31441 attcggtatt ctgaaaataa ctgttcgtgc gactccaggc gttgtaaaac aacgccttgt
    31501 tcatccattc aaagtcaaag gcgagagtga ttgcttcccg gacccggcga tcgctgaata
    31561 ccggacgttg gatattaaac gccagccagc gcgtatcctg ggctgattcg ttcttttgct
    31621 catctttgat gatgtatttt ttatcgaaat ttttaccggt ataacgcgtg gcccagtttt
    31681 tggcgtcgtt ttccatacgc aaatcaaagg cacctgcttt aaacgcttca aaggcgacat
    31741 tatcatcgag gtaataatcg tagcgaatgg tgtcgaaatt ccagcgtccg cggtttaccg
    31801 gtaagtttgc tgcccagtaa tctttcacgc gggaatagac aatattttgc cccattttcc
    31861 aggacgtaat gcggtacgga ccactggcaa gcggaggcgt ggcgagcggg tcgctaagtt
    31921 tgtgatcctt ccagtacttt tctggaaata ccggcagcga aaacagactc aacatatctt
    31981 ctttgccggg tttagctaac tcaatgcgca cggttaacgg cgcaatggct ttgacggtgg
    32041 tgcctttgta gaccagacga aattgcggca cgccttcggt cataaatttt tgaaaagtaa
    32101 actctacatc gcgggcagta atgggcgaac catcatgaaa acgggcgcgc ggattaatag
    32161 cgacctccac ccaggaataa tcgtcagcat agcgtgcgct ttcagcaatc agcgggtaat
    32221 aactgcctgg ttcgtcatcg gaagtcgtaa atagcgtgtc gtacagctgc tcggtgcgtg
    32281 cgcccgggtt gccgcgcagt gcatagcggt tgaaattatc gaaggtgccg agggctgaca
    32341 acgttatctg cccacctttt ggcgcggcgg ggttcacata atcaaaatgg ttgaaattaa
    32401 acgcgtaccg gggttcgccc agcacggcaa aggcatagct ttccttgata gcctgcgcct
    32461 gcacaccaaa ggtgaacaga gcgataaaca gcagcagtat gcgcacaatc atctgcaacg
    32521 gatttccttt tactcatcat tagccgcaat ccggcatgct ttcactatga ataataaggg
    32581 acgtaagcac accttaccac tgcggcgtat acggtttctt cagccagcgg acaaaatcgt
    32641 ccagcggtaa cgggcgacta atccagtagc cttgcatgaa attaacgccg cgttcgctta
    32701 gccatcgcgc ttgttccggc gtttcgaccc cttcagcaac tgtcagcata ttgagacgtt
    32761 tcgccagcgt cagcaccgcg tcaagtacgg gtgaagtgat cgtttccgta ccgatggcgt
    32821 tgataaatcc acggtcaatt ttcagataat cgagcgtaaa acgctcaaga tagataagcg
    32881 cgctgtgccc ggtgccgaag tcatcaatag caatttctac gccgaccgag tgcagccagg
    32941 cgaagagttg tgtggcttct tgctctttca gcatatcgcg ctcggtaatt tccagcacaa
    33001 tctggaaatg gtgtgcgggt agggaagtga gcagtttctg gatatctgct ttaaagcttt
    33061 cgctgtgcag atggtccggc gcaatgttaa taccaaattt gacgcctact ggcagcactt
    33121 tttctaattc tgcggcatcg cgggcaatta actcaaacag gtgctgagtc agcggcacaa
    33181 tcatcttttg cgattcggca aagttaatga aggcatccgg gggaatttct cccgcgacag
    33241 gatgccgcca gcgtagcagt acttccaggc ccgttactcg caaagcttgt gtatccacca
    33301 ccggttgata cgccacgtaa aattgttcgc gcttgatggc ggtcatgatt tctctgccgg
    33361 ggcgcatacg tacgctcatc aggtaatagc agagcaggcc aacgacagtt ccactcatgc
    33421 cgcccagtaa aaatgcgtac cacacatcgt tccatgtcca gtcatctgca taaagccgaa
    33481 cggtcagagg aatgcccgca attttagttt cacggactgg catgtcggtt aattcgttaa
    33541 cgttcatcaa acgtgaagaa aaggtagata gcgcagtatt accaataatg agggcgacgc
    33601 catcgtaatc ttcctgccgt gaactataaa agagagaagg cgtcaggttg agattcagag
    33661 cggcaaagac gccgctattt ttcagcaaag ggttgcgata ccagatgacg attgcgggtt
    33721 tgttcggcac catcggcgtg ccgggtaaga tcgccatatc gacgttttta ttaatgtcga
    33781 gcgccggaat caattcattg agtggaatgt ccatctcacc ggtcgcagat gaacagaatg
    33841 tttttttatc tttcaccagc acaaacgtac ggacattcat gctaaacgcc gcgcgggcgg
    33901 tcagttcggg gttagcttgc tggcaggtat ctaaggtcag cggctggagc cggtcagtag
    33961 tggatttcag atcggcaaaa taggtatcga gatacttttg tacgtcggta atcagtgtgt
    34021 cgtatttgac ttcgtgctta tgccaggcca ctaaaaattg aaggcaactc accagtatcg
    34081 caatcatcac gcctgcaaca atgcaggtaa gcaggagctt acgtccagaa ttgggagcgc
    34141 gtatgaacat gattccgtta atttcctgaa attacgacaa catttaaaag ataacaatga
    34201 ccggactata gcctggatca agacgcggaa acatcatttt ctctgatgaa tgaaaacaaa
    34261 aaagcactgc ctaagcagtg ctttcagtta actcgtcagg atagccaagg gattgcatcc
    34321 aaacggttta ttagctgcgg ctgagaaccc ggcgtgcttc gttgtaacgc ttcttccagt
    34381 acggttcatt catgctggaa ataataacac cactgctggt ggaagcatgg acaaactgat
    34441 tgttgccgat ataaataccg acatggcgtc ccgttgaacc ggcacggaac agaactaaat
    34501 cacccgtacg caaattactg cgggaaacag atttacccat ttcctgctgt tcgtaagtcg
    34561 aacgcggaag ttctaagcca aattgctcac ggaatgtacg ctgtacgaaa ccagaacaat
    34621 cgataccttt tttagtgctg ccgcccagac gataacgtac gcctttccag tcagcatact
    34681 gatccataat tcgcgatttt acgtcgacat tacgaaccag gttttcaaat tcatcctgag
    34741 aagcttgcag tgatgatgtt tcactaccca ctgcacgtgt ctcaggatgc atattctttg
    34801 cggtgttatt tgcactacat gcagaaagca gaaccgctac tgcaatcgcg ggaatcccgc
    34861 gcaagatata tctcaaaatc ggttgagatt tgaccatgtt gtttgttttc ccttgaagtc
    34921 cttaacgaca aatatcgtta taaaaaaatg ccaagcgaca cgagactaat tacctgggca
    34981 tgttgaaaca attccttaag gccaaatctg tggactaacg cacaaaatta tatgctgtga
    35041 ggataatcta ccgccgaggc aaaacgagtt cgagattacc cgattggtgc ggtcttggcg
    35101 agcggtttta catgattttt tataagaaaa agtgatacag aaagttaata agcgggattg
    35161 gtcaattaaa gtacgattga gtgaaacgca tgcaatccat tcatttactt agagaatgat
    35221 ttttgcgcgc gatggatgaa agcacggtgc gcatcatcag gattatcctc actataaaaa
    35281 taagcctgat gatgttaatt actgtgagtt atttgttttg gaaatgtttg ttttttcccg
    35341 gtagtgattt gtcaaaaaag gtgattaatc gatcacttaa tggcgtcagc aacacccagg
    35401 gcaaaccgct caatatcacg gtcatcgaac cgacaatgat gtcagtaaac cagtgtgcgc
    35461 caatcattac tctgggaaat gcaaaaacca caaaaataat aagggcgata aggcctgcaa
    35521 ctttgccgaa ataacgccac atgaatgccg aaaaaataag cagcatcatg ccgtgatcgc
    35581 cgggaaagct atctcgtgag gcatctttcg tgggaacaga gagcagttcg ctgacgcggt
    35641 taatatcggt aaaagtcaat gttgggctgg cccgttttac aggaattaat gcctgaccca
    35701 gctggtttaa taccactgca gttaatagca tgaccagacc cataatcacg atacgtcgtc
    35761 tgccaggggc gttttctttc agccagaaac tcagcatcaa cattcccatc gccagcagag
    35821 aacaaccgtc gaaagcgcga ttgttggtca atgcaaccag ccacaaaaag gccttacttt
    35881 cgaccagttt ctgattaaag aaataaaaaa tatccgcatc aattggcaac cagaatccat
    35941 gattaacggg aatataccag gaaagaaaca gcgtgaggcc gacaatattc aacaacacta
    36001 tttgcggcaa atttttaatc atattttctc taattatctt actgaaaaac gcaggcaacc
    36061 ttacgcggta gtcgctaaac gaagcttcaa cagcgcgctc tgcaaagcat tccagtcagc
    36121 ttcgctgctg gaaatcagct caatacggct gtccggtggc gcaacgtttt gcgtttcaat
    36181 gtgcaggtca tcgccctgac ggttgattcg taccagccct tcgggaatac gcagcacgcc
    36241 tttgacgcgt tccaccggtg caagtcgcgc ccattccaga atgccaatgg tgtcgaatac
    36301 cgtatcagcg tcgaatatcc agccgcaggc ctgatatcct tgcccgctgt tcagactgcg
    36361 acgccagcgt tgatgctctg gcaggcttaa cgctgctaac ccttttttca cgacatgctg
    36421 atgagaatgc gcggcgctgg cgggcaactc ggctaaattg cgacgcggca aatccagaag
    36481 atgaccgtca actttcccat gctcactgtg aattaattgt cgatcgccac cattttgctg
    36541 ccaccaacgc tgtagcgctt gctcactttc gggcgtcgca cggtcggatt tattggcgac
    36601 aatgatgtct gcggcagcca gctggtcacg gaagttttca ttgctggcgc ttttttcgtc
    36661 cagcagcaag cgcggatcga gaatgcacaa ggtggcgcgc agatctatcc acggttcata
    36721 gactggtgcg gttaacagat cgaggatctg tttcggatgg cccagcccgg tcggctctat
    36781 caacaagcgg tctggttttc cctgacgcag taaggtattc aaccctacct gcatgggtaa
    36841 accattaacg cagcacatgc agccgccggg gatctctttc agcaatgcgc cgctatcggc
    36901 gagcaaagca ccatcaattc cgacttcccc aaattcatta accagtaccg cccatttttc
    36961 gttgggatct ttatgggcta acagatgaag aatcgacgtg gttttcccgc tgccgagaaa
    37021 accggtgatg aggttggtcc tggtcacaac ggctccgcaa tactctggta agttaactga
    37081 gcgttaatcc tggctaaaaa aggcagaaaa gagaagggga gagcggacga aggtgagctt
    37141 catccgcttt aaccgcagaa atcgttaaat tttgagggtg tttaatagcg cctgatgtgc
    37201 gccgaattgt acgatttgtt gccaggcttg ttcgatggcc tgcacaaaat gcgggttatc
    37261 tggcagatca tcaccgaaaa cttcacgcag ggaaagcagg gcggttacgc gttgttcact
    37321 gctgctgccc gcaacaagtt cgcgaatttt atcgctaagc ggatcgcgaa catcaatggc
    37381 atttccggca tcatcaacgc cgctgacgta acgcatccag ccagcaacgc ctaatgccag
    37441 caacgaccag tccgtttcac gccccagatg tatgcgaata cctgccagca tgcgttgcgg
    37501 taatttctgg ctgccatcca tcgcgatttg ccaggtctta tgtttcagcg acggattagc
    37561 aaaacgtgca attaacttat ccgcatattg tgttaaatcg acatctttaa tttgcagtgt
    37621 cggcgcttgc tcatccagca ttaatgttct ggcagcatgg cgaaatacgc gatcctgcat
    37681 gcaatcactg atatgggcga atcctgagag gtaacccaga taagcgagaa aagagtggct
    37741 gccattaagc atacgcagtt tcatctcttc ccatggcagg acatcattca ccatttgtac
    37801 acctgcgact tcccaggcag gacgcccagc gatgaagtta tcttcaacca cccactggat
    37861 aaacggttcg cagctaatcg cgcagggatc atttaccccc agatgctggc tgatttctgc
    37921 cagtgattcg tcggttgcag ccggaacaat gcggtcgacc atggttcccg gaaaactgac
    37981 gtgttctttt atccacccgg cgagttctgg cgaacgtttt tctgccattc ccagcaccgc
    38041 gtttttcacc acatggccat tgtcgggaat attgtcgcag gagagcacgg taaacggtgt
    38101 aaggccgcgc tcacggcggc gtttcagtgc ttcgacgaga atacccggtg cggagtgagg
    38161 ttcttcaggg gtttgtagat cgtgaataat ccgcggatta ctggtgtcga gtgcaccggt
    38221 ggccgggtca atacaatagc ctttttcggt aatcgtcagg gaaacaattg ccacctgtgg
    38281 ctcgcaaaat ttctcaataa ttgccgctaa ggaatccagt tttgcattaa ggcattcgtg
    38341 aacggcaccg acaattatca cctgattgcc gtccgcacct ttttccagca cggtatataa
    38401 atggttctgt gcgcggagct ggctcatcag ttgatcaccg ctgaacaagc tgatttcaca
    38461 gatcccccag tcgccgccct gggcattcag cacacgatcg gtcagtaacg cctgatgagc
    38521 gcggtgaaag gcgccaaagc caaaatgaac gatacgtgat tgcaactgct ggcgatcgta
    38581 gcgtggagcg tgtacatgat gcgggagcgt aacggaggca attgtcttca taggagtcac
    38641 gtcttagtaa cgataaatta acaatgacag tgtaaagtta tatgacagca attgaaattg
    38701 gtctggattg ttaatgcgat gaagtgtgag gacgatcaat caacgagtga aaattgaggg
    38761 cgtaaggtgt tcaggaagcg caaaatagaa gattggtata ataaattaaa aaagggacag
    38821 cctgagctat cccttttctg catgcggctg aagttagtca gcacgcccca tataacggcg
    38881 ttcttcgata tggatacgaa ttttttcgcc cgggcttaag tattctggta cctgaatcac
    38941 cagaccagtg ctcaatgtcg ccggtttgtt acgggcgctg gcagatgccc ctttgatgcc
    39001 tggtgcggtt tcaacgattt ccagatcaac ggtctgcggc agctcaagcg ccagcagttg
    39061 accgtcccag gtcagcacct gcatgtccgg catgccgccc tctggcataa acagcaactc
    39121 ttcttcaatc tgatctttgg tgaaggtata cggggtatag tcttctttat ccataaagac
    39181 atattcgttg ccatcgacat aggagaagtc aacgtaacgg cgggtcagcg tcacggtgtc
    39241 aacgatatca tcacctttga aacgctcttc tactttcagc ccggtacgga catcagaaaa
    39301 acgcattttg tacagcgtag cggcaccgcg ggcagtgggc gactgaatat caatatcctt
    39361 aaccagcagc agtttgccgt tgtaattcag taccatacct tttttaattt cgttcgctct
    39421 tggcatcgaa aaattcctgt agtcgaaaat gtcaaaaata tcgcgacaaa gtactcgcga
    39481 cgggcctttc gggcaagtgg tattcgcact tttgctggtg caaaaaaggt ggtactgtgc
    39541 gcgctcatca atccggtggt taaccttaag agaacaacgt atgaaatgcc gtccgggttg
    39601 cggggcgtgt tgcactgccc cttcaatttc cagccctatt cccggtatgc cagatggcaa
    39661 gcccgccaat acgccctgca ttcagcttga tgaacagcaa cgctgtaaaa ttttcacctc
    39721 gccactgcga ccgaaagtct gtgcaggctt acaagccagc gctgaaatgt gcggtaactc
    39781 gcgtcagcag gccatgacct ggcttatcga tctggaaatg ctgaccgcgc cttaaacatc
    39841 tttaatccgc agtaagcaaa acagagtggc gataatcatc accatcgcaa accagaacac
    39901 agcgtgataa ttccagatct cggcgacgat tcccgccact gatcctgcga tgatccagcc
    39961 cacgcgcgac gtgttggtat agagcgtggt ggctgaaccc gcctgaccgg gcatcagatc
    40021 ctgaaaatag agcatcccga tgccgcccag aatgccaata aaaatagcat ttagcagctg
    40081 caagcccaac agaatgacag gtgaatgcgc catcagcatt cctgcgtaaa aacagacgcc
    40141 acccacggca gcaacgcgca ttaagaaacg cttacccaga cgtttggcga aatatccggc
    40201 aatcaacatc gtcgggattt ccagcccggc ggcggtcccc atcatcacac cggccagttt
    40261 ctcgggaaga tgcagttcgt tgataataaa tagcggcatg ttgatgatgt acaggctgtt
    40321 cgagccccac atcaatgtac aaatgacaaa cagcagcagc gtatcgcgac ggttacggcg
    40381 cggcgcttcg atcgtgccgg tcgccagcgg aagctctttt tgcatcgacg gtaaaaacag
    40441 ccacaccatc acaccgcaaa caataaacgc taccgctgcg ctcagataca ttaccgtaaa
    40501 gctgaaaccc atcgctaagg cataagccag cggtgggcca atgacccatg ccagtgaaac
    40561 ctgagcgcgt aaaaaagagc tgaacatcac cgcctcacgt ccggttttgt cggcatgttc
    40621 acgggcaagg gcaaacattt gcgggttagc ggtcgagcca aagctgctaa gaaagacgcc
    40681 aacgaatagc aaaacaaagt agttgcgatt ccaggcaaaa agggtgcagg ccagcacgcc
    40741 taacaggcag caaaagacaa tcagcgattt gcgatcgccg cgcttatcag agcgcccggc
    40801 gagaaactga cttaccagaa tcccaatgac agcgctgccg gtaaagaaga atcccaccat
    40861 cgccggacga gcatgtactt catcagtaag aaaaatactg agtgtcgggg tttgcagagc
    40921 gcccgcaata ccggtgagaa aggcaacgat taaaaacgcc gtcgaggtca ggtcaaacga
    40981 tttcgcgctg gagactgcgg gggagttatg catgctggtc gatcactgga agaggaagac
    41041 gcggagttta cgctgcttgt cagaaaataa acagacagcg aaatcaaaag cgccacgaat
    41101 aatcaaaatg ccatttcact gttgctgatt ggctgctgac gctgctgaaa tgaaattgct
    41161 gataacggat tttcccatca gcaattagga aaaatggcaa aaaattgtgc agcacatcaa
    41221 acttttgctc ataactttac ggctttcctt gcgtgctgaa acagaagtat tatgctttct
    41281 tgaaacgttt cagcgcgatc ttgtctttaa ccctaagcca ggttggcgct ttttttctca
    41341 tagaggctga atcgtttcaa ttcagcaaga gaggagaact atgttccagt tatccgtaca
    41401 ggacatccat ccgggcgaaa aggccggaga caaagaagag gcgattcgcc aggtcgctgc
    41461 ggcgctggtg caggccggta atgtagcaga aggctacgtc aatggcatgc tggcgcgcga
    41521 acagcaaacc tcaacgttcc tcggcaatag tattgctatt ccacacggca ctaccgacac
    41581 ccgcgatcag gtgctgaaaa ccggcgttca ggtatttcag ttcccggaag gcgtcacctg
    41641 gggtgacggt caggtagcgt acgtggcaat cggtattgct gccagctcgg atgaacatct
    41701 gggcctgcta cgccagctga cccacgtact gagcgatgat tccgttgctg aacaactgaa
    41761 gtcagcaaca acagcagaag aacttcgcgc attactgatg ggcgaaaagc agagtgagca
    41821 gctgaagctc gacaacgaaa tgctgacgct ggatatcgtc gccagcgatc tgctgactct
    41881 tcaggcgctg aacgctgcgc gtctgaaaga ggcgggggca gttgacgcca ctttcgtcac
    41941 caaagccatc aatgaacaac cgctgaatct cggacagggt atctggctga gcgatagcgc
    42001 cgaaggcaat ctgcgtagcg cgattgcggt aagccgtgcg gcaaatgctt ttgatgtgga
    42061 cggcgaaacg gcagccatgc tggtgagtgt ggcgatgaat gacgatcagc ccatcgcggt
    42121 tcttaagcgt ctcgctgatt tattgctcga caataaagct gaccgcttgc tgaaagcgga
    42181 tgcggcaacg ttgctggcgc tgctgaccag cgatgatgcg ccgaccgacg acgtgttaag
    42241 cgcggagttt gtggtgcgca atgaacacgg cctgcatgct cgtccaggta ccatgctggt
    42301 caataccatt aaacaattta acagtgatat taccgtgaca aaccttgatg gtaccggcaa
    42361 accggcaaac ggacgtagtc tgatgaaagt tgtggcactt ggcgttaaga aaggtcatcg
    42421 cctacgcttt accgcccagg gtgcagatgc tgaacaggcg ctgaaagcca ttggcgacgc
    42481 tatcgctgct ggtcttgggg agggcgcata atgagcagac gtgttgctac tatcaccctt
    42541 aatccggctt atgaccttgt tggtttctgc ccggaaattg aacgcggcga agtgaacctg
    42601 gtgaaaacca ccggtctgca tgcggcgggt aaaggcatca acgtggccaa agtattaaaa
    42661 gacctgggaa ttgatgtcac cgttggcggc ttcctgggta aagacaatca ggatggtttt
    42721 cagcaactgt tcagcgagct gggcattgcc aaccgtttcc aggttgtaca ggggcgcacc
    42781 cgaattaacg ttaagctgac ggaaaaagac ggcgaagtga ccgacttcaa cttttcgggt
    42841 tttgaagtca cccccgccga ctgggaacgc tttgtgactg attctctgag ctggctcggt
    42901 cagttcgata tggtctgtgt cagcggaagc ttaccgtcag gcgtcagccc ggaagcgttc
    42961 accgactgga tgactcgcct gcgtagtcag tgtccttgca ttatctttga tagtagccgt
    43021 gaagcgttag tagcaggttt gaaagcggca ccgtggctgg tgaaacctaa ccgccgcgag
    43081 ttggaaatct gggcaggccg taaactgcct gaaatgaaag atgtgattga agctgcacat
    43141 gcgctacgtg aacaaggtat tgcgcatgtt gttatttcac tgggtgccga aggcgcgctt
    43201 tgggttaatg cctccggcga atggatcgcc aaaccaccgt cagtcgatgt cgtaagcacc
    43261 gttggcgcag gggattctat ggttggtggc ctgatttatg gcttgctgat gcgtgaatcc
    43321 agtgaacaca cactgcgtct ggcgacagct gttgcagccc tggcggtaag tcaaagcaat
    43381 gtgggtatta ccgatcgtcc gcagttggcc gcaatgatgg cgcgcgtcga cttacaacct
    43441 tttaactgac agcaggagag gcataatgaa aacgctgctg attattgacg ctaatctcgg
    43501 tcaggcacgc gcctatatgg cgaagaccct gctgggcgcg gcggcgcgaa aagcaaaact
    43561 ggaaatcatc gacaatccga acgacgcaga aatggcgatt gttctcggtg attccatccc
    43621 gaatgacagc gcgctgaacg gtaaaaatgt ctggctgggt gatatttccc gggcagttgc
    43681 gcaccctgag ctgttcctga gtgaagccaa aggccatgcg aaaccttaca ctgcgccggt
    43741 cgctgcgaca gcaccggttg ctgccagcgg tccgaaacgc gtagttgcgg tgactgcttg
    43801 cccgactggc gtagcacaca cctttatggc ggctgaagcc attgaaaccg aagcgaaaaa
    43861 acgtggctgg tgggtgaaag ttgaaacccg tggttctgtt ggcgcgggca atgcaatcac
    43921 ccctgaagaa gtcgcagcag cggatctggt gattgtggcg gcagatatcg aagtggatct
    43981 ggcgaaattt gctggtaaac cgatgtatcg cacctctacc ggtctggcgc tgaagaaaac
    44041 tgcgcaggaa ctggataaag cggttgctga agcaacgccg tatgaaccag cgggcaaagc
    44101 tcaaacagcg accactgaag gtaagaaaga gagtgcaggc gcttaccgtc acttgctgac
    44161 gggcgtctct tacatgctgc cgatggttgt tgcaggtggt ctgtgtatcg cgctttcttt
    44221 tgcttttggt atcgaagcgt ttaaagagcc gggtacgttg gcagcggcgc tgatgcagat
    44281 tggtggtggt tcagcctttg cgctgatggt gccggtactg gcaggttata ttgccttctc
    44341 cattgccgat cgtccgggcc tcacaccggg tctgattggc ggtatgctgg cggtcagcac
    44401 cggttctggc tttattggcg gtataattgc tggcttcctg gctggttaca ttgcgaagtt
    44461 aatcagtacg caattgaaac tgccacagag tatggaggcg ctgaaaccga tcctgatcat
    44521 tcctctaatt tccagtctgg tggtcggtct ggcgatgatc tacctaatcg gtaaaccggt
    44581 tgctggcatt ctcgatgggt tgactcactg gctgcagacc atggggactg cgaatgcggt
    44641 tctgctgggg gcgatcctcg gtggcatgat gtgtactgac atgggcggtc cggtaaacaa
    44701 agcagcgtac gcattcggtg tgggtctgct gagtactcaa acctatggcc cgatggcggc
    44761 gattatggcg gcaggtatgg tgccaccgct ggcaatgggt ctggcaacaa tggtggcgcg
    44821 tcgcaaattc gacaaagcgc agcaggaagg tggcaaagcc gctctggtac ttggtctgtg
    44881 ctttatttcg gaaggtgcaa ttccgtttgc tgctcgtgat ccgatgcgtg tgctaccgtg
    44941 ctgtatcgtg ggtggggcgc tgactggcgc aatctcaatg gcgattggtg cgaaactgat
    45001 ggcaccacac ggtggtctgt ttgttctgct gatccctggc gctattacgc cggtattggg
    45061 ttacctggta gcaattattg ccggtacgct ggtggcgggt ttggcctatg ccttcctgaa
    45121 acgtccggaa gtggacgcag tagcgaaagc agcgtaataa aaggtgtgtt acagggcaga
    45181 aatttcctgc cctgtaacat ttgatgagcg caaagagaat gaccagcttg tgaatgtaat
    45241 tttatgaatt taaatggttt tatctttcag tagaaacttc aaacgttcat caaacactaa
    45301 ggttatttcc tcaagggcag attattttct gccctttttt agtgttcctg ttctgtgcat
    45361 cccgtcacaa attcctcgct tttgtaattc aggcatttaa tcgcattttg tgagcctgct
    45421 caaatctgcc cattttgcct ctctggcggt aatttacttt acgtaaaaca tgcatactat
    45481 gagcacatgt ttaaatatga acacatgttt aaaccgtctt tgcagtatct atattcttgt
    45541 ggattgacag ggttggtaat tttttaccag aggacgtgct atgcgcgaaa aggattatgt
    45601 cgtaattata ggttcggcga atattgatgt cgccggatat tcacatgaat cattaaatta
    45661 tgcggattca aatccaggta aaataaaatt tacgcctggt ggagtagggc gcaatattgc
    45721 acaaaacctg gcgttgctgg gtaacaaagc ctggctactg agcgcagtag gcagtgattt
    45781 ttatggtcaa tcgctgctaa cgcaaaccaa tcaatctggc gtttatgtcg ataaatgcct
    45841 gattgtgccg ggagaaaata cgtctagtta tttatcatta ctcgataata ccggtgaaat
    45901 gctggttgct ataaatgaca tgaatattag caacgctatt acagctgaat atctcgcaca
    45961 gcaccgtgaa tttattcaga gggcaaaggt cattgtcgcg gactgtaata tcagtgaaga
    46021 ggcactggca tggattctgg ataatgccgc caacgtaccc gtatttgtcg atccggtttc
    46081 cgcatggaaa tgtgtcaaag tgcgcgaccg tctaaatcag atccacactc tgaagccaaa
    46141 ccgccttgaa gcggaaaccc tgagtgggat tgcgctgtca gggcgtgacg atgtggcaaa
    46201 agttgctgcc tggttccatc aacatggcct gaaccgactg gtattgagca tgggcggcga
    46261 cggcgtttat tacagcgata tcagcggtga aaatggctgg tctgcgccga tcaaaaccaa
    46321 tgttattaat gttaccggag cgggcgatgc catgatggcg ggacttgctt cgtgttgggt
    46381 agacggaatg ccgtttgccg aatctgttcg tttcgcacag ggatgttcgt caatggcgct
    46441 ctcctgtgaa tacaccaata accccgattt atcgattgcc aacgttatat cgttagtgga
    46501 gaacgcagaa tgtctaaatt aaaaatttcc cctgaattat tacaaatttc cccggaagtg
    46561 caggaagctt taaaaaacaa aaaaccggtt gtggcgctgg aatcgaccat tatttctcac
    46621 gggatgccgt tcccacaaaa tgcccagacc gcaattgaag tagaagaaac tattcgtaaa
    46681 cagggcgcag tacctgccac tatcgccatt attggcggcg tgatgaaagt gggtttaagc
    46741 aaagaagaaa ttgaattact gggtcgtgaa gggcataacg tgaccaaagt tagtcgtcgc
    46801 gatttacctt ttgttgttgc cgccggaaaa aatggcgcaa ccactgtggc ttcaacgatg
    46861 attattgcgg cgcttgccgg aattaaagta tttgccaccg ggggaattgg tggtgtgcat
    46921 cgcggggcgg aacatacctt cgatatttct gccgatttgc aagaactggc aaatactaat
    46981 gtcaccgttg tttgtgccgg ggcgaaatct attctcgatt taggattaac cactgagtat
    47041 ttagaaacct tcggtgtgcc gttaattggc tatcagacta aagcgctgcc tgcgtttttc
    47101 tgccgtacca gctcgtttga cgtcagcatt cgtctcgaca gcgccagtga aattgcccgt
    47161 gcaatggcgg tgaaatggca aagcgggctg aacggtggcc tcgtggtagc gaacccgatc
    47221 ccggaacagt ttgcgatgcc ggaggaatct atcaatgcag ccatagatca agccgtcgcc
    47281 gaagccgaag agcagggcgt tattggtaaa gaaagtacac cgttcctgct ggcgcgcgtt
    47341 gctgaactga ccggcggtga tagcctgaaa tccaatatcc agctggtgtt caacaacgcc
    47401 attctggcga gtgaaattgc caaagaatac cagcgtctcg cgggttaatt tctgacgggc
    47461 agaaagccgt cgcccgtact catcaagcat accaaaacct ggcgctgacg ccgggtttac
    47521 tggcatgaag ggaaaatcat tatggatata atgagaagtg ttgtggggat ggtggtgtta
    47581 ctggcaatag catttctgtt gtcagtgaat aaaaagagca tcagtttgcg cacggttgga
    47641 gccgcactgc tgctgcaaat cgctattggt ggcatcatgc tctacttccc accgggaaag
    47701 tgggcagtag aacaggcggc attaggcgtt cataaagtga tgtcttacag tgatgccggt
    47761 agcgccttca tttttggttc gctggttggg ccgaaaatgg atgtcctgtt tgacggtgcg
    47821 ggttttatct tcgcctttcg cgtacttccg gcgattattt tcgttactgc gctcatcagt
    47881 ctgctgtact acattggcgt gatggggctg ctgattcgca tccttggcag cattttccag
    47941 aaagctctta acatcagcaa aatcgaatct tttgtcgcag tcacaactat tttcctcggg
    48001 caaaatgaga tcccggcgat cgttaaaccg tttatcgatc gcatgaatcg caacgagttg
    48061 tttaccgcaa tttgtagcgg gatggcgtcc attgctggtt cgatgatgat tggttatgcc
    48121 ggaatgggcg taccaattga ctacctgtta gcggcatcgc tgatggcgat cccaggtggt
    48181 attttgtttg cacgtattct tagcccggcc accgagcctt cacaggtcac atttgaaaat
    48241 ctgtcgttca gcgaaacgcc gccaaaaagc tttatcgaag cggcggcgag cggtgcgatg
    48301 accgggttga aaatcgccgc cggtgtggcg acggtggtaa tggcgtttgt cgcaattatt
    48361 gcgctgatca acggcattat cggcggaatt ggcggctggt ttggtttcgc caatgcctct
    48421 ctggaaagta tttttggcta tgtactggca ccgctggcgt ggattatggg cgtggactgg
    48481 agtgatgcca accttgcggg tagcctgatt gggcagaaac tggcgattaa cgaattcgtc
    48541 gcttacctga gtttctcccc atacctgcaa acgggcggca cgctggaagt gaaaaccgtg
    48601 gcgattatct cctttgcgct ttgtggtttt gctaactttg gttctatcgg tgttgtcgtt
    48661 ggcgcatttt cggctatttc gccaaaacgt gctccagaaa tcgcccagct tggtttacgg
    48721 gcgctggcag cagcaacgct ttccaacctg atgagcgcga ccattgccgg gttctttatt
    48781 ggtttagctt gagtttcatt gccggatgta cgcgcttatt aagcgttgca tccggcaatc
    48841 gcgctattgc aaataatttt tactgcatca tcccggagaa tttattc
//
LOCUS       DAZKIV010000016        48837 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZKIV010000016 DAZKIV010000000
VERSION     DAZKIV010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.016"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..282)
                     /gene="chbB"
                     /locus_tag="ACHOT6_000970"
     CDS             complement(<1..282)
                     /gene="chbB"
                     /locus_tag="ACHOT6_000970"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFP9946229.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLK"
     gene            complement(581..919)
                     /gene="osmE"
                     /locus_tag="ACHOT6_000971"
     CDS             complement(581..919)
                     /gene="osmE"
                     /locus_tag="ACHOT6_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFP9946230.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1121..1948
                     /gene="nadE"
                     /locus_tag="ACHOT6_000972"
     CDS             1121..1948
                     /gene="nadE"
                     /locus_tag="ACHOT6_000972"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFP9946231.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2178..2960
                     /gene="cho"
                     /locus_tag="ACHOT6_000973"
                     /pseudo
     CDS             2178..2960
                     /gene="cho"
                     /locus_tag="ACHOT6_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
     gene            complement(2956..3363)
                     /gene="ves"
                     /locus_tag="ACHOT6_000974"
                     /pseudo
     CDS             complement(2956..3363)
                     /gene="ves"
                     /locus_tag="ACHOT6_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
     gene            complement(3566..4051)
                     /gene="spy"
                     /locus_tag="ACHOT6_000975"
     CDS             complement(3566..4051)
                     /gene="spy"
                     /locus_tag="ACHOT6_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFP9946232.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4381..5349)
                     /gene="astE"
                     /locus_tag="ACHOT6_000976"
     CDS             complement(4381..5349)
                     /gene="astE"
                     /locus_tag="ACHOT6_000976"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFP9946233.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5342..6685)
                     /gene="astB"
                     /locus_tag="ACHOT6_000977"
     CDS             complement(5342..6685)
                     /gene="astB"
                     /locus_tag="ACHOT6_000977"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFP9946234.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6682..8160)
                     /gene="astD"
                     /locus_tag="ACHOT6_000978"
     CDS             complement(6682..8160)
                     /gene="astD"
                     /locus_tag="ACHOT6_000978"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFP9946235.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8157..9191)
                     /gene="astA"
                     /locus_tag="ACHOT6_000979"
     CDS             complement(8157..9191)
                     /gene="astA"
                     /locus_tag="ACHOT6_000979"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFP9946236.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKGFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9188..10408)
                     /gene="astC"
                     /locus_tag="ACHOT6_000980"
     CDS             complement(9188..10408)
                     /gene="astC"
                     /locus_tag="ACHOT6_000980"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFP9946237.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            10854..11660
                     /gene="xthA"
                     /locus_tag="ACHOT6_000981"
     CDS             10854..11660
                     /gene="xthA"
                     /locus_tag="ACHOT6_000981"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFP9946238.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            11827..12537
                     /gene="ydjX"
                     /locus_tag="ACHOT6_000982"
     CDS             11827..12537
                     /gene="ydjX"
                     /locus_tag="ACHOT6_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9946239.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12542..13219
                     /gene="ydjY"
                     /locus_tag="ACHOT6_000983"
     CDS             12542..13219
                     /gene="ydjY"
                     /locus_tag="ACHOT6_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFP9946240.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAGKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            13234..13941
                     /gene="ydjZ"
                     /locus_tag="ACHOT6_000984"
     CDS             13234..13941
                     /gene="ydjZ"
                     /locus_tag="ACHOT6_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFP9946241.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            13941..14489
                     /gene="ynjA"
                     /locus_tag="ACHOT6_000985"
     CDS             13941..14489
                     /gene="ynjA"
                     /locus_tag="ACHOT6_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFP9946242.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14499..15665
                     /gene="ynjB"
                     /locus_tag="ACHOT6_000986"
     CDS             14499..15665
                     /gene="ynjB"
                     /locus_tag="ACHOT6_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9946243.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLCLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            15638..17173
                     /gene="ynjC"
                     /locus_tag="ACHOT6_000987"
     CDS             15638..17173
                     /gene="ynjC"
                     /locus_tag="ACHOT6_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFP9946244.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17173..17826
                     /gene="ynjD"
                     /locus_tag="ACHOT6_000988"
     CDS             17173..17826
                     /gene="ynjD"
                     /locus_tag="ACHOT6_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9946245.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMEQWSENYNKLR"
     gene            17893..19200
                     /gene="ynjE"
                     /locus_tag="ACHOT6_000989"
     CDS             17893..19200
                     /gene="ynjE"
                     /locus_tag="ACHOT6_000989"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFP9946246.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19209..19820)
                     /gene="ynjF"
                     /locus_tag="ACHOT6_000990"
     CDS             complement(19209..19820)
                     /gene="ynjF"
                     /locus_tag="ACHOT6_000990"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9946247.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            19907..20314
                     /gene="nudG"
                     /locus_tag="ACHOT6_000991"
     CDS             19907..20314
                     /gene="nudG"
                     /locus_tag="ACHOT6_000991"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFP9946248.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20280..20552)
                     /gene="ynjH"
                     /locus_tag="ACHOT6_000992"
     CDS             complement(20280..20552)
                     /gene="ynjH"
                     /locus_tag="ACHOT6_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFP9946249.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQCQRDDKTLSTNPLVWRRVKP"
     gene            20788..22131
                     /gene="gdhA"
                     /locus_tag="ACHOT6_000993"
     CDS             20788..22131
                     /gene="gdhA"
                     /locus_tag="ACHOT6_000993"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFP9946250.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22248..23288)
                     /gene="ynjI"
                     /locus_tag="ACHOT6_000994"
     CDS             complement(22248..23288)
                     /gene="ynjI"
                     /locus_tag="ACHOT6_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9946251.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGG
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLEDQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANHSSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(23415..25377)
                     /gene="topB"
                     /locus_tag="ACHOT6_000995"
                     /pseudo
     CDS             complement(23415..25377)
                     /gene="topB"
                     /locus_tag="ACHOT6_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
     gene            complement(25382..26425)
                     /gene="selD"
                     /locus_tag="ACHOT6_000996"
     CDS             complement(25382..26425)
                     /gene="selD"
                     /locus_tag="ACHOT6_000996"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFP9946252.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(26542..27093)
                     /gene="ydjA"
                     /locus_tag="ACHOT6_000997"
     CDS             complement(26542..27093)
                     /gene="ydjA"
                     /locus_tag="ACHOT6_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFP9946253.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            27254..29110
                     /gene="sppA"
                     /locus_tag="ACHOT6_000998"
     CDS             27254..29110
                     /gene="sppA"
                     /locus_tag="ACHOT6_000998"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFP9946254.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            29277..30293
                     /gene="ansA"
                     /locus_tag="ACHOT6_000999"
     CDS             29277..30293
                     /gene="ansA"
                     /locus_tag="ACHOT6_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFP9946255.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            30304..30945
                     /gene="pncA"
                     /locus_tag="ACHOT6_001000"
     CDS             30304..30945
                     /gene="pncA"
                     /locus_tag="ACHOT6_001000"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFP9946256.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASLDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(31038..32396)
                     /gene="ydjE"
                     /locus_tag="ACHOT6_001001"
     CDS             complement(31038..32396)
                     /gene="ydjE"
                     /locus_tag="ACHOT6_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946257.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLNAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLAGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFWLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSIQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(32513..33271)
                     /gene="ydjF"
                     /locus_tag="ACHOT6_001002"
     CDS             complement(32513..33271)
                     /gene="ydjF"
                     /locus_tag="ACHOT6_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9946258.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAMLTENIHFYKRASSFYEEKQLIARKALPFIDNKTTMAADSS
                     SAVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            complement(33408..34388)
                     /gene="ydjG"
                     /locus_tag="ACHOT6_001003"
     CDS             complement(33408..34388)
                     /gene="ydjG"
                     /locus_tag="ACHOT6_001003"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HFP9946259.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTAPGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVAASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKSE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            complement(34398..35345)
                     /gene="ydjH"
                     /locus_tag="ACHOT6_001004"
     CDS             complement(34398..35345)
                     /gene="ydjH"
                     /locus_tag="ACHOT6_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9946260.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMTTGGDA
                     INEATIISRLGHRTALMSRIGKDAAGQFILDHCRKDNIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFSQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEAKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            complement(35350..36186)
                     /gene="ydjI"
                     /locus_tag="ACHOT6_001005"
     CDS             complement(35350..36186)
                     /gene="ydjI"
                     /locus_tag="ACHOT6_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HFP9946261.1"
                     /translation="MLADIRYWENDATNKHYAIAHFNVWNAEMLMGVIDAAEEAKSPV
                     IISFGTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSMEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIKLFGSDGK
                     AE"
     gene            complement(36207..37250)
                     /gene="ydjJ"
                     /locus_tag="ACHOT6_001006"
     CDS             complement(36207..37250)
                     /gene="ydjJ"
                     /locus_tag="ACHOT6_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HFP9946262.1"
                     /translation="MKNSKAILQVPGTMKIISAEIPVPKEDEVLIKVEYVGICGSDVH
                     GFESGPFIPPKDPNQEIGLGHECAGTVVAVGSRVRKFKPGDRVNIEPGVPCGHCRYCL
                     EGKYNICPDVDFMATQPNYRGALTHYLCHPESFTYKLPDNMDTMEGALVEPAAVGMHA
                     AMLADVKPGKKIIILGAGCIGLMTLQACKCLGATEIAVVDVLEKRLIMAEQLGATVVI
                     NGTKEDTIARCQQFTEDMGADIVFETAGSAVTVKQAPYLVMRGGKIMIVGTVPGDSAI
                     NFLKINREVTIQTVFRYANRYPVTIEAISSGRFDVKSMVTHIYDYRDVQQAFEESVNN
                     KRDIIKGVIKISD"
     gene            complement(37267..38644)
                     /gene="ydjK"
                     /locus_tag="ACHOT6_001007"
                     /pseudo
     CDS             complement(37267..38644)
                     /gene="ydjK"
                     /locus_tag="ACHOT6_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(38671..39747)
                     /gene="ydjL"
                     /locus_tag="ACHOT6_001008"
     CDS             complement(38671..39747)
                     /gene="ydjL"
                     /locus_tag="ACHOT6_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9946263.1"
                     /translation="MKALARFGKAFGGYKMIDVPQPICGPEDVVIEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            complement(40117..40389)
                     /gene="yeaC"
                     /locus_tag="ACHOT6_001009"
     CDS             complement(40117..40389)
                     /gene="yeaC"
                     /locus_tag="ACHOT6_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFP9946264.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(40431..40844)
                     /gene="msrB"
                     /locus_tag="ACHOT6_001010"
     CDS             complement(40431..40844)
                     /gene="msrB"
                     /locus_tag="ACHOT6_001010"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFP9946265.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            41186..42181
                     /gene="gapA"
                     /locus_tag="ACHOT6_001011"
     CDS             41186..42181
                     /gene="gapA"
                     /locus_tag="ACHOT6_001011"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9946266.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            42265..43149
                     /gene="yeaD"
                     /locus_tag="ACHOT6_001012"
     CDS             42265..43149
                     /gene="yeaD"
                     /locus_tag="ACHOT6_001012"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFP9946267.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(43200..44054)
                     /gene="yeaE"
                     /locus_tag="ACHOT6_001013"
     CDS             complement(43200..44054)
                     /gene="yeaE"
                     /locus_tag="ACHOT6_001013"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFP9946268.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDHADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(44144..44890)
                     /gene="mipA"
                     /locus_tag="ACHOT6_001014"
     CDS             complement(44144..44890)
                     /gene="mipA"
                     /locus_tag="ACHOT6_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFP9946269.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            45326..47260
                     /gene="yeaG"
                     /locus_tag="ACHOT6_001015"
     CDS             45326..47260
                     /gene="yeaG"
                     /locus_tag="ACHOT6_001015"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFP9946270.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            47373..48656
                     /gene="yeaH"
                     /locus_tag="ACHOT6_001016"
     CDS             47373..48656
                     /gene="yeaH"
                     /locus_tag="ACHOT6_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFP9946271.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT        12145 a        12756 c        11877 g        12059 t
ORIGIN      
        1 cttaagcacg cctaaaccat cgactttgcc ataaagcagc gagtcaatta cttcaaccgg
       61 tttgttgggt aacaaacgct ggatttcggg caacatataa gcaatctgcg gccctaataa
      121 cacgacatcg gcattctgac ctttttcacc agccagtgtt tccggaaatg cttcaataat
      181 gaccggaact tcatattttt ctgcctgtgc gcgcattttt gataccagta aagaggtaga
      241 catgcccgca gaacaaaaca gataaatgtg tttcttttcc ataaaactgc cctcatcgac
      301 gattatctgt cagccagaca ctccgcaagc cttaacctgc ttccatgctc tgggtaactt
      361 gcgaaaccaa catgactttt tttgtggctg aaaggagtat acggtataga cctgagcaat
      421 agtatttctt tgaccttata aattgtctct ccttgacctg cggttatgac cctcttcaca
      481 tttcgggcaa ataattcgcg aagataatta agaactcagg ccaaaaaaaa ccggcacaat
      541 ggccggtttc cgttgttact caagctttca gacgaattga ttacttcgca gcctgtggat
      601 cagtgtcgta ttcagcacag gtctgataac cggagttgat gacatgtccg gtatcatcta
      661 acgcgacaaa gtaggtttct gctttaccat cacgttgacc caggatgtag gtctggcaag
      721 taccacgagc atggatcatg ctcacttcag acgaaggttt acccgcaatt tgtgcaacct
      781 gcgcccggct catgcctttt ttgacgtctt tcaccacagg ctgtacaaac tggtctttag
      841 tacgatcata agccgtacaa cccgccagca tggttaatac cgccgctgca ctcagaattc
      901 ctgccatatt cttgttcata ttccgtcctc ttgtttatca gcgtgttaga taagcctgga
      961 atacattggg cgctttttca agcccgtgaa cgaaacggct ccgctttcag aggattcctg
     1021 tatgacgttt taaccaccat tcagcccgct gtcgcttgtc gtttcagtgg caacgggtta
     1081 gctttaagga agttttgtct tttctgtctg gaggggttca atgacattgc aacaacaaat
     1141 aataaaggcg ctgggcgcaa aaccgcagat taatgctgaa gaggaaattc gtcgtagtat
     1201 cgattttctg aaaagctacc tgcaaactta tccgttcatt aaatcactgg tgctcgggat
     1261 cagcggcggt caggactcca cgcttgccgg aaagctgtgc cagatggcga ttaatgagct
     1321 gcgcctggaa accggcaacg aatcactgca atttattgcc gtacgcctgc cctatggtgt
     1381 tcaggccgac gaacaagatt gccaggatgc cattgccttt attcaaccgg atcgcgtatt
     1441 aaccgttaat atcaagggcg cggtattggc cagcgaacag gcattgcggg aagcaggcat
     1501 tgaactgagc gattttgtcc gtggcaatga aaaagcgcgt gagcggatga aagcacaata
     1561 tagcattgcg ggtatgacca gcggtgtcgt ggtgggcacc gatcatgcag cagaagccat
     1621 taccggattc ttcactaaat atggtgacgg cggtacggat attaatccgc tgtatcgtct
     1681 caacaaacgc cagggtaaac agttgctgac ggcattaggt tgcccggaac acctttataa
     1741 gaaagcccca acggccgatc tggaagatga tcgcccttct ctgccagatg aagtggcact
     1801 cggcgtgacc tatgacaata tcgacgacta tctggaaggg aaaaacgtac ctgaacaggt
     1861 cgccagaaca atagagaact ggtatctgaa aaccgaacat aaacgccgtc cgccaattac
     1921 cgttttcgat gatttctgga aaaagtaata atttgcacat aatggtttgt gcgaaaaaga
     1981 gtaatttgtt cacgccggat gcggcgtgaa cgccttattc gacctacaaa actatgcaaa
     2041 ttcaatatat tgcaggagtc gaataggcct gacaggcgta gcacgtcaga cggtgtaacc
     2101 tttgtcatcg acccgcctct tttttaatcg cttcccgcct gttacactgg atagataacc
     2161 agcattcgga gtcaacagtg gtacggcgtt taacttctcc gcggctcgaa tttgaagctg
     2221 cggcaattta tgaatatccc gaacatttac gttcattcct taatgactta cccacccgac
     2281 caggggtgta tctgtttcat ggcgaaagtg acaccatgcc gctctatatc ggcaaaagca
     2341 ttaacatccg cagccgcgtc ctttctcatt tacgtacccc ggatgaagcc gccatgctac
     2401 ggcaatcccg acgcatcagc tggatctgca ccgcaggcga aatcggcgct ctgctccttg
     2461 aagcgcgatt aatcaaagaa caacagccgc tgtttaataa acggttgcgc cgcaatcgcc
     2521 agctctgcgc cctgcaatta aatgaaaagc gcgtcgatgt ggtgtatgcc aaagaggtgg
     2581 atttttcacg agcccccaac ctgtttggcc tgtttgccaa taggcgcgca gctttgcaag
     2641 cattgcagag catcgctgat gaacaaaaac tttgttatgg cctgttggga ctggaaccgt
     2701 taagtcgcgg tcgtgcatgt tttcgttcag cgctaaaacg ttgcgccgga gcatgctgcg
     2761 gtaaagagag ccatgaggaa catgcgctac gcttgcgcca gtctctggag cgtttgcggg
     2821 tggtgtgttg gccttggcaa ggtgcggtgg cactgaaaga acagcacccg gaaatgactc
     2881 aatatcatat tattcaaaac tggctgtggc tgggggcggt taattcgctg gaagaagcga
     2941 caacgttaat tcggacacca ccccgccgcg tgagccaaag gtagtaaacg tacgttcggc
     3001 aattctgact ttcgccttac agacatccag gcgtgtcatg atgttgaaat ccatcgacat
     3061 ctgccctgcc gtcagtttcg cttttaccac ctggtccgct gcaaaggcaa aaggctgtaa
     3121 cggttttaag gtatggttaa agcggtctgc gctttcaagg agcatctcgc cgccttccag
     3181 caacgtcact atcctttcca tgccgggaaa caaagaaaac tccccattcg ccgcgatgga
     3241 tgcaatgctg gcacgccagt aaaaatcacg ttttgccggt gggaaagtgc aaatttcgcg
     3301 cgtttcaccg gcagcatttc gccacagatt caccgacatt ttacgcatat caaagtattc
     3361 catactcgct cccggcccgt tcttttcatt gttatcgttc aggtaaccgt ggatggttat
     3421 cggcaactcg ggtgatttac gttagtggtg atcaggcttt ttttacaact gtttgagaag
     3481 agaaaagaaa accgccgatc ctgtccaccg cattactgca aggtagtgga caagaccggc
     3541 ggtcttaagt tttttggctg aaagattatt cagcagttgc aggcatttta ccttttgccg
     3601 ctggacgttc tgtcagacgc ttctcaaaat tagcattaaa ttgctttttc tgttccggcg
     3661 tcaggatgtt gtaaattttg ttctgggttt ccatgtgcgc cagcatgtta gctttgcgct
     3721 gttcttccat ttttgcgatc tgcgcttcag cttttgcttt atcgaaggta tcgctggcaa
     3781 tgatgtcatg cattgcgcgg cgttcttcca gcggcggacg tttcatctgg tcacgctggc
     3841 ctttcatgat ttcgcggatc tgctgtttct gcgcgtcggt caggttcagg tctttgaaca
     3901 tcatgtcctg atgcggaccg aacttgcctt tgtggtgcat catcggcttc gcgtcagccg
     3961 gtgctgcggt agtggtgtct gcggcatggg ccaggttagc cgcgccaaga gccagggtag
     4021 aggcaacaaa cagtgcagtt aatttacgca tattctatat ccttcctttc agttatttat
     4081 tacggctttc ttaagtagcg tgccgtgttg acgagattaa ctttactgac tttagcgtca
     4141 attaatcaga gcaacggtaa aacaatgaaa gtgtaaaaaa cacttttgcg ccaattatgg
     4201 agaaaaaaag aaaatttgat ggagagtgat gagagaatat tacaacacga tgattttgca
     4261 gagattatga agaactatac cggatgactg gtgataaata aagcaaataa ccaggattaa
     4321 tctgtattaa tttataagaa agcaacttaa tacccgcaga atgatttctg cgggtaagta
     4381 ttagcttatt ttttcgagca ttaatcccgc gcgtaatccc aacgctacca acggattagg
     4441 gaataacaca tactctacat catgggttac ggtaaaacgt tcctctccgt cctgcgccag
     4501 caatgttcct ttctcaaacg gcataaaatt cagcgtgtca cttgccatat gcatttcgaa
     4561 ggacggcgag tggcgagtaa tttgcgaaac cacccgataa cggagcggcg gtgttctcac
     4621 gataccgaca ctctcaccag atagcagcgc agcaattgcg ctggcagtta ctgcaaactg
     4681 gcgaagatcg ttttgcccaa agggcaacgc tttgccaagt tccagcgtac aggccagcgc
     4741 gccaaaatgt ctggcgctga aatgggtaaa cgtaccacca ggttcctgat ggaacaccag
     4801 cgcctccagc cccgccgcac ccagccacgt cagaaatttc tcgtcccagg gaatgtcgcg
     4861 ttgcggtaat acaccgaact gcggatgcaa ggagccacga attgcggtat gtagatcaag
     4921 gtgccagcgc acagattctt tgcccaggtc ataaaaatct tccaggcact gttccagttc
     4981 gcgcgcccga caggtttctc cgctttcagc aaatagctgc caacgaccgc caaacattcg
     5041 attcatatcg ctatggcaat aacgtttccc ttgcttcagc gcaggaggat tcccgaggat
     5101 caccagcaac cgccaacgta acgggatctc gccgtgagat atcgcgccaa gcaacgcgtc
     5161 cagcatctcc acaggtgccg tctcattacc gtgtattccc gctgaaatca ccagtgcgcc
     5221 ttgcggtggc gttaatggcg tcagttccag cacaccatcg cccagccagc gccagcgaac
     5281 gccgttgatt tctcgctcgg tgataaccgg ttttttaccc gttaaggtca gagcaagaaa
     5341 attatccatt gccccctccc tcgcgctgga acggataaac cgaaccgaga ttcagtaatt
     5401 ggctcaatac atccagtgct tcccgccctc cgcgcagcaa ttgcgggtcg gccagatcgg
     5461 cagcagtaag gcgatcgcgg tagtaacgat ccacccagtc attgagcgca ttaaacagcg
     5521 tatcgttcat catcaccgcc ggattcaccg cccggcgttc ttcttctgtc aataccaccc
     5581 gcaaccgcag gcacgccggg ccgccgccat tcgccatgct ttcacgtaaa tcaaagactt
     5641 ttagttcgct aatcgggttg tcagcggcaa ggagttcatt gagataaccc catactccgg
     5701 cgtgttcccg acactcctga ggcagcacca acatcatgga accatcatcg cggctcaaca
     5761 gttggctgtt aaacagatag gtagacaccg catccgacac ggaaacctga gttgccggaa
     5821 cttctatcgc cataaaaccg ttcacccgcg cacgcaggtt tgccagtaac tgtgactggc
     5881 gagcgaacgc ctgttggtgg caaaacagca cctggcggtt actcacggca atcacgtcat
     5941 tatgaaaaac gccctggtcg ataacgtccg ggttttgctg ggcgaaaatc acctgttggg
     6001 gattcacctg attcagcctt gccaccgcct cgctggcttc gcgagtctgt cgcgccggat
     6061 aacgggaagg ccgggtatca ttgccttctt ctcgcccgta gacaaaaagt tgcatacccg
     6121 gctcaccgta atgaccgccg agacgattgt ggtttgccgc cccctcatca ccgagcaacg
     6181 ctacctgcgg caacgccgaa tgaacgctaa atttctcttc gtcgttaaaa atcgctttta
     6241 aaagtgattc ggtgacgtgc gcttccagcg agcggtgaaa tttattgttc aggttggcaa
     6301 cggtgagatg cactttgcca tccagcgtat cggcagatgg cgcgatcgtt gccgcattgg
     6361 ctacccacat tggcgaagcg gaactgacgc tggaaagcca gtgcggtgcc tggcgtgcaa
     6421 ctttttccag tacctgctca tcgctaccac tgaatcccaa ctgacgcagc accggaataa
     6481 acggacgctc gtgcggcggg atcacggcct gggggaatcc cgcatcggca agggctttca
     6541 ttttcagtaa gccctgtttt gccgcctgtc gcgggttaga cacctgaaaa cggtgacggg
     6601 tagaggcttc attaccaaac gacaggccag cgtaatgatg cgtcagccct accagcccgt
     6661 cgaaattaac ttcccaggcg ttcatcgcac cacctcatcg gaaaaatcca gcccggggtt
     6721 aagcgttgcg ggcaaagtta acgagtccga ctccaggctc gccataggcc atgcgcagta
     6781 atctgcggca taccaggcgc tggggcgatg gttaccggat gcaccaatgc cgccgaatgg
     6841 cgcggtactg gcagcaccag taagcggttt gttccagtta acaatccccg cccgcgcctc
     6901 cagcaacagt tgatcgaatt tttcccgctc gggggaaacc agaccgcaag agagtccgaa
     6961 gcgagtgtta ttcgccatta gaatcgcttc ctcgaaagaa tcataacgcc agacgcgcaa
     7021 taacggtccg aacacctctt catctggtac gccagcaacg cctgtcattt caatgatccc
     7081 cggcgtcagc aacgatgtct ctgattgtaa taagcgcggc gcaagcaggg ttcgtccgcc
     7141 catcgcttcc agttgctgcc aggcagtaac cacctgctgt gcggcctgtt cagaaatcag
     7201 cccgccaata aacggctgcg gttcgtcatc ccagttgccc ggcgttaatc gctggctgac
     7261 ggcaaccaga cgagcaagaa acgcatcgcc ctgcgctccg cttttgagca ataaacggcg
     7321 ggcgcaggtg cagcgttgcc cggctgtgac aaacgccgac tgaatggtca gatggacagc
     7381 cgcgtcgata tccgccacct catcgataat tagcggatta ttaccgccca tctcaagggc
     7441 gagaattttc tccggctgac cggagagctg gcgatgcagc tggtagccgg tattggcgct
     7501 accggtaaac agcaaaccgt cgagatcctc cagcgcactc agcgcctgac ccgtttcacg
     7561 cccgccctgc accaggttca gtacgcccgg cggcaagcca gcctgctgcc ataaacgcat
     7621 taccgcttcg ccactccacg gtgtcagttc gctgggttta aagataatgg tgttacctgc
     7681 cagcaatgcc ggaacgatat gtccgttcgg caaatgacca gggaaattat acggcccaaa
     7741 caccgccagc acgccgtgcg ggcgatgtcg caggctcgcc gcaccgtccg gcatttcact
     7801 acgctgctcg ccggtacgaa cgtgatacgc cttaattgat atcgcgattt tattgatcat
     7861 cgccgtcact tcggttgccg cttcccagcg cggcttaccc gtttctctgg caataatcgc
     7921 ggttaattcg cctttattcc tttccagcaa tccggcaaag cgttcgacaa cgacctgacg
     7981 ttcagccaat gagagccgcg cccagcgcgg aaacgccgca cgggctgccc gacaagcctg
     8041 ttcgacctga gcggcatcgg catcattgcc ttgccataac acctcgcccg ataccggatt
     8101 acgcttcaca cgcgatgcgc cctggcccgt tatccagtca ccgttaatcc ataaagtcat
     8161 gctgttttct cctctgcgca caggcgcacc agacgaacgc gatcccctgc gtggcatttg
     8221 agggcatcca gttgtgcggc ggttaaaatc aaacgctcgg ttgccggatc ggtacgcgcc
     8281 agcaccacgc ggaaatggtg ataattttca ttggcgacca ggcaggccgg aaaatcgccc
     8341 tgcgcaggct gcccttctgc cacttccacc agccgacttt tacgaatggc gcgcacgcgg
     8401 tcgatgtcac actcaagcgt tggcccgccg tcgaagatgt cgatatagtt gcggtaacga
     8461 aaaccttctt tctccagcac cgcgcgggca ggcgcggttt gcggatgtac ctgaccgatg
     8521 acgtcctggg cttcctggga taaaaagtgg gtatagatcg gatgtttcgg catcagttct
     8581 gcaataaatg ccttttgccc ggtgccgcag agaaaatcgg cgcggctaaa atccatcgaa
     8641 aagaagcctt taccgaggct ttgccagaac ggtgaatagc cgtgttcgtc aatcaccccg
     8701 cgcatttcag caaccacttt gtcattaaac ttgtcgcgaa aagccgccat aaacataaag
     8761 cgcgatttcg acagcaaata gccgttgccc tctttgcgcc agtccgggtc gagaaacagc
     8821 gtgcacagct cgctgctgcc ggtgtgatcg ttactgagaa acagcgtcgg caatgcgtta
     8881 tagacattca gctcttttga ggcgtgaacc aacgtgccga cgcgatagtt gtaccaggga
     8941 tcgttcagcc caaccgccac ctcaatggca caaatccccg ccacggtgcc tgtctcgcta
     9001 tcttccagca cgaacacata gccctgctca cttttgggca gttcgccttg ccaggttttg
     9061 attgcccttt cgatacgcgc cgaaagcgtg gcttcattgg cgggaagcga cgtcaggccg
     9121 ccgcccgttt tgctggcaag ctgcatcagc gccgagacat ctgatcgctc aacgggacgg
     9181 atgaccatca tgatgaacct cggctaacaa agtgttcgca agcagctgca aagcgatcca
     9241 gtccggtcgt cacctcttct tcgctgacat tgagcgcagg cgcaaaacgc accacgttgc
     9301 cacccgcaat cagtaccatc acgcctgctt tcgccgcttc ctgagagatc tgtttcgctt
     9361 gcccggcgta atcggcattc agtacacagc caatcagcaa acctaagccg cgaacttcac
     9421 tgaacaaacc atagcggtga ttaatagtat taagacgctc aacaaaccag tcgtgacgct
     9481 gtttaacgcc attaagcatc tctggtgtgt tgatgagctc cagcactttg cctgccaccg
     9541 ccgaggccag cggattaccg ccataggtgg tgccatgagt gccaacggtc atcacgcggg
     9601 cgcactcttc ggttgccaac aacgcaccga ccgggaaacc gccgcccagc gctttggcgg
     9661 tagttaacag atcaggcgtc acgccgtagt gcatataggc atacagttcc ccggtgcgcc
     9721 cgacgccggt ttgtacttca tcaaaaatca acagcgcatt gtggcggtta cacaattcac
     9781 gcagaccttg taaaaacgcg ttgctggctg gcaccacacc gccttccccc tggatgggtt
     9841 cgacaatcac cgcacaggta gagtcgtcaa tcagcgcgct ggcagagtta atatcgttat
     9901 atgcagcatg acgaatatcc gccggcagtg gcgcaaaatc ctgtgaatag gctggctgcc
     9961 cccccgcact gacagtaaac agcgtgcgac catgaaacgc atttttgaac gccacgatgc
    10021 cgctcttatg gctgccgtag cggtcgtgag cgaatttacg cgccagtttt agcgccgctt
    10081 cgttggcttc cgcaccggag ttacaaaaga agacgcgatc ggcaaacgtg gcgtcgatca
    10141 attttttcgc cagtcgcagt accggctcgt tggtgtaacc gttgccggta tgccagaact
    10201 tactcgcctg ttcgttcagc gcttcacgca gttccggatg cgcatggccc agcgcgttca
    10261 ccgcaatgcc acccgcgaag tcgatatact ctttcccctg ctgatcccac aagcgcgaac
    10321 cttcgccacg taccggtata aagggtgccg gagcgtaaac aggtatcatc cattcatcaa
    10381 agttttcacg cgtaattggc tgagacatag cgacctctac agtaaataat tcgttattta
    10441 tatgttaaca ataagtaatg tttgcgctgt aaatgtagat tgcagggttc gtgccagcca
    10501 gtgataaaag tgcataaacg gcggaggcta actggaaatc aaggagttat aaccaaacca
    10561 tatgcattta aagtgcatat aaagtgaata cgtttgcgat gtgggtgaat aaaaagaata
    10621 aaaaacgcaa tgttatgcag aagtgaaata taattctgga attgtgatca ttgacgaaat
    10681 ttactggaaa ttactgcgcc attctgacgc agcgcgcacc aaaagcgggc attttttgcg
    10741 ccatcgttga catcattaac aaccatcgat caaatcactt aacaacaggc ggtaagcaac
    10801 gcgaaattct gctaccatcc acgcactctt tatctgaata aatggcagcg actatgaaat
    10861 ttgtctcttt taatatcaac ggcctgcgcg ccagacctca ccagcttgaa gccatcgtcg
    10921 aaaagcacca accggatgtg attggcctgc aggagacaaa agttcatgac gatatgtttc
    10981 cgctcgaaga ggtggcgaag ctcggctaca acgtgtttta tcacgggcag aaaggccatt
    11041 atggcgtggc gctgctgacc aaagagacgc cgattgccgt gcgtcgcggc tttcccggtg
    11101 acgacgaaga ggcgcagcgg cggattatta tggcggaaat cccctcactg ctgggtaatg
    11161 tcaccgtgat caacggttac ttcccgcagg gtgaaagccg cgaccatccg ataaaattcc
    11221 cggcaaaagc gcagttttat cagaatctgc aaaactacct ggaaaccgaa ctcaaacgtg
    11281 ataatccggt actgattatg ggcgatatga atatcagccc tacagatctg gatatcggca
    11341 ttggcgaaga aaaccgtaag cgctggctgc gtaccggtaa atgctctttc ctgccggaag
    11401 agcgcgaatg gatggacagg ctgatgagct gggggttggt cgatactttc cgccatgcta
    11461 atccgcaaac agcagatcgt ttctcatggt ttgattaccg ctcaaaaggt tttgacgata
    11521 accgtggtct gcgcatcgac ctgctgctcg ccagccaacc gctggcagaa tgttgcgtag
    11581 aaaccggcat cgactatgaa attcgcagca tggaaaaacc gtccgatcac gcccccgtct
    11641 gggcgacctt ccgccgctaa tttagcagct ctcctggctc aaactgggtc aggagaatta
    11701 accttgagaa aaatcaacaa actgtcagta atgatttgtt gcctgccgtc gtttgttata
    11761 ccgtcactgc gtttttagtt gtctgaccac ttctctatta tcaagtttga tataggaaac
    11821 tccacgatga acgctgagcg taactttctg tttgcctgtc ttatttttgc gctggtcatt
    11881 tacgctatcc acgctttcgg tttattcgat ctgctcaccg atttacccca cttacagaca
    11941 ctcattcgcc agagcggatt gttcggttat agcctctata ttctgttatt catcattgcc
    12001 accctctttc tgttaccagg aagcatactg gtgatcgctg gaggaatagt ttttggcccg
    12061 ctcttaggga cactactctc attaattgcc gcaacgctgg cctcctcgag ctcattcctg
    12121 ctggcgcgct ggctgggacg tgatttactg ctgaaatacg tgggccatag ccataccttc
    12181 caggccattg aaaaaggcat tgcgcgtaac ggtattgatt ttcttattct gacccgctta
    12241 atcccgttgt ttccttacaa tattcaaaat tacgcttacg gattaaccac catcgccttc
    12301 tggccttata cccttatttc ggcactcacg accctgcccg gtattgttat ttataccgtg
    12361 atggcaagcg atctcgccaa tgaaggcatt acgctgcgct ttattttaca actctgtctg
    12421 gcgggcctgg cgctgtttat tctcgtccag ctcgcaaaac tctacgcccg acacaaacat
    12481 gtggatctgt ctgcttcgcg ccgcagccca cttactcacc caaaaaatga aggatagaac
    12541 gatgtcgcaa cattattcag tgtcatggaa aaaaggactg gctgcactct gcttactggc
    12601 tgttgccggg cttagcggct gcgatcaaaa agagaatgcg gcgggaaaag tggaatacga
    12661 cggactttcg aacagccaac cgctgcgcgt cgatgccaat aaccatacgg taaccatgct
    12721 ggtgcaaatt aatggtcgtt tcctcaccga cgacactcgt cacggtattg tgtttaaaga
    12781 tggctccaac ggacataaat cgctgtttat ggcttatgcg accccgaaag cattttatga
    12841 agccctgaaa gaggcaggtg gtacgccggg cgaaaacatg acgatggata ataaagaaac
    12901 gactcttgtc acaggcagca aactggatat ttcggtcaac tggcaagggg cggcaaaagc
    12961 gtattccttc gatgaagtga ttgttgacag taatggcaag aaactggaca tgcgctttgg
    13021 cggtaattta acggcagcag aagagaagaa aacgggttgc ctggtgtgtc tggatagctg
    13081 cccggtcggc atcgtcagca atgcaacata cacttatggt gcggtggaaa aacgcggcga
    13141 agtgaaattc aaaggcaatg cctcagttct cccggcggat aacacgctgg caacggttac
    13201 ctttaaaatc accgaataaa gccaggataa aggatgatga agatacaatc gcgtaaaatc
    13261 tggtactacc gtataaccct catcatcctg ttgttcgcta tgctgctcgc atgggcgctg
    13321 cttcccggcg tccatgagtt tatcaatcgc agcgttgcgg cgtttgccgc cgtggaccaa
    13381 cagggtatag aacgctttat tcagtcttac ggcgcactgg cggcagttgt ctcgttcttg
    13441 ttgatgattt tacaggccat tgctgcaccg ctacctgcgt ttttgatcac ctttgccaat
    13501 gcgtcgctgt ttggcgcgtt ctggggcggc ttgctgtcgt ggaccagttc gatggccggc
    13561 gcggcgctgt gcttttttat cgccagagtg atgggccgcg aagtggtgga aaaattaacc
    13621 ggcaaaaccg tgcttgacag tatggacggc tttttcactc gctacggcaa acacaccatc
    13681 ctggtctgtc ggttattgcc ttttgtccct ttcgatccaa tcagctatgc tgccggtctg
    13741 acttcaatac gttttcgctc gttttttatc gccaccgggc ttggtcaatt accggcgact
    13801 attgtttatt cctgggcggg cagcatgtta acaggcggta ctttctggtt tgtcaccgga
    13861 ctgtttattc tgtttgccct gaccgtggtg atttttatgg cgaagaaaat atggcttgag
    13921 cgccagaaga ggaatgcctg atgggtttac cgccgcttag caaaattcct ttcattttac
    13981 gtccgcaggc gtggctgcat cgtcgccatt acggcgaggt gctaagcccg attcgctggt
    14041 gggggcggat cccgtttatc ttttatttgg tgtcaatgtt tgttgggtgg ctggagcgca
    14101 aacgctcacc gctcgatccg gtagtgcgat cgcttgtcag cgcgcgcatt gcgcaaatgt
    14161 gcctgtgtga gttttgcgtg gatatcacca gtatgaaagt cgccgagcgt accggcagca
    14221 ccgataaact gctggcagtg gctgactggc ggcaaagccc gctctttagc gatgaagaac
    14281 ggctggcgct ggagtacgcc gaagccgcaa gcgtcacacc gccaacagtc gatgatgccc
    14341 tgcgtacccg actggctgcg cattttgacg ctcaggcgct caccgaactg acggcattga
    14401 tcggcctgca aaacctgtcg gctcgtttta attctgccat ggacattccc gctcaggggc
    14461 tgtgccgtat tcctgaaaaa cgttcttaag gagagatgat gcgccattgt gggtggttgc
    14521 tgggattgtt atcgctgttt tctttggcaa cacatgccag tgactggcag gaaattaaaa
    14581 atgaggccaa agggcaaacc gtctggttta acgcctgggg cggcgatacc gcaattaacc
    14641 gctatctcga ctgggttagc ggcgagatga aaacccatta cgctataaac ctgaagattg
    14701 ttcgtctcgc ggatgccgca gacgcggtga agcgcattca gaccgaagct gctgccggtc
    14761 gtaaaacggg cggctcggtg gatctgctct gggtgaacgg cgaaaacttc cgcaccttaa
    14821 aagaggccaa tttattacaa acgggctggg cggagactct gcccaactgg cgctatgtcg
    14881 acacacagct gccggtgcgg gaagattttt cagtgccgac acaaggtgcg gaatcgccct
    14941 ggggcggcgc acaactgacg tttatcgccc gccgcgatgt tacgccacag ccaccacaaa
    15001 cgccgcaagc cttactggag tttgctaaag ccaatcccgg cacggttact tatccgcgcc
    15061 caccggactt taccggcacg gcgtttcttg aacagttgct gattatgctg acgccagatc
    15121 ccgccgcatt aaaagaagcg ccggacgatg cgactttcgc ccgtgtcact gcgcccttgt
    15181 ggcaatatct tgatgcactg catccgtatt tgtggcgcga aggaaaggat ttcccgcctt
    15241 cgcccgcgcg gatggatgct ctgctgaaag ccggcacatt gtgcctgtcg ctgaccttta
    15301 gccccgcgca tgcgcagcaa aaaatcgcca gcggcgattt gccggcaagc agttacagtt
    15361 ttggctttcg cgaggggatg ataggcaacg tgcatttcgt caccattcca gccaacgcga
    15421 atgccagtgc tgcggcgaag gtagttgcca atttcctgct ctcacccgat gcgcaactgc
    15481 gtaaagcaga tcccgttgtc tggggcgatc cttctgttct cgatccgcaa aaactgcctg
    15541 acgggcagcg cgaaatattg caatcaagaa tgccgcagga tctgccgccg gtactggctg
    15601 aaccgcacgc aggatgggtg aatgcactgg aacaagaatg gttacgccgt tacggtacgc
    15661 attaatcttt ttgctgtggg cgatggtggc ggtgatttat gcaccgctga tcccggcagc
    15721 tctcacgctg atatcgcctg cgttgtcgtt gacacactgg caggcgttat ttgccgatcc
    15781 acagttaccg catgcattac tggcaacgct ggtgtcgaca accatcgcgg cggtcggggc
    15841 attgttgatt gccctgctgg tgattgtggc gctatggcct gggccgaaat ggcagcgtat
    15901 gtgcgcccgt ctgccgtggc tgctcgccat tccccatgtg gcttttgcca ccagcgccct
    15961 gctgctcttt gctgacggag ggctgctata tgactatttc ccgtatttca ctccgccaat
    16021 ggaccgattt ggcatcggtc tgggcctcac ccttgcagtg aaagaaagcg catttctgct
    16081 gtggatctta gcggcagtat tgagcgaaaa acggctgttg cagcaggtca ttgtgctgga
    16141 ttcgctgggc tacagccgct ggcaatgcct gaactggctg ctgttgccct ccgtcgcgcc
    16201 ttcgctggca atggcgatgc tggcgattgt tgcctggtcg ctttcggtcg tggatgtggc
    16261 gattattctt gggccaggta atccaccgac gctggcagta attagctggc agtggttaac
    16321 tcagggcgac gcggatcaac aaacgaaagg cgcactcgcc agtctgctgc tgatgctgtt
    16381 actcgccgcc tacgttttgc tgagctatct gctatggcgc agctggcggc gcactattcc
    16441 ccgcgtagat ggcattcgca agcctgccac acctttatta ccaggcacta cgctggcgag
    16501 ttttttaccc ttaaccggtg tgctgtgtgt ggttctgctg gcgatcctcg cggatcagtc
    16561 gacgatcaat agtgaagcac tcatcaacag tctgacaatg gggctggtag caacattcat
    16621 cgctttgctc ctgttactgc tgtggctgga atgggggcca cagcgtcgcc agttgtggct
    16681 atggttgccc attctattgc ccgctctgcc actggtggcg ggccagtaca cgctggcgct
    16741 atggctgaac ctggatggaa gctggacggc ggtggtctgg gggcatctgc tgtgggtaat
    16801 gccgtggatg ctgtttatcc tgcaacccgc ctggcagcgc attgattcac ggttaatttt
    16861 gattgcgcaa acactgggct ggtcgcgggc caaaatcttc ttttacgtga aatgcccact
    16921 catgttgcgc cctgtgctga ttgccttcgc ggtgggattt tcagtcagta ttgcgcagta
    16981 tatgccgacg ctgtggctgg gcgcggggcg ttttccgacg ctcaccactg aggcggtggc
    17041 attaagcagc ggcggcagca acggtattct cgccgcccag gctttatggc aactgttgtt
    17101 accgcttatt atttttgccc tgaccgcgtt agtcgcaaaa tgggtaggtt atgtcagaca
    17161 aggactccgc taatgctctg cgtgaaaaat gtttcgctac gtttaccaga aagccgcttg
    17221 ctgacaaacg ttaactttac ggttgataaa ggtgacatcg tcacgttaat ggggccgtct
    17281 ggctgtggaa aatccactct gttttcatgg atgattggtg cactggctgg acagttttct
    17341 tgtacaggtg agctatggct caatgagcaa cggattgaca tgctacccac cgcacagcgt
    17401 cagattggca ttcttttcca ggatgcactg ttatttgacc agttcagcgt cgggcaaaat
    17461 ttactgctgg cgctaccgtc gacgctcaag gggactgcca ggcgcaatgc cgtgaaagac
    17521 gcgcttgatc gagcgggact ggctgaaact tatcaccagg accctgcgac attatcaggc
    17581 ggtcagcgag cgcgggtggc cctgctgcgc gcccttctcg ctcagccaaa agcgttactc
    17641 ctggatgagc cattcagccg tcttgatgtg gctctgcgcg ataattttcg ccagtgggtg
    17701 ttcagcgaag ttcgcgaact ggcgatcccc gtcgttcagg taacgcacga tctccaggat
    17761 gttcctgctg atagttctgt tctggatatg gagcagtggt cagaaaatta caacaaactg
    17821 cgataacgca aagtttttct caatgcgtca gttcagaatg gcgcactcaa aactacaatg
    17881 tcgggatttt cgatgaaacg tgtttctcaa atgaccgcgc tggcaatggc tttagggctg
    17941 gcttgcgctt cttcgtgggc cgctgaactg gcgaagcctc ttacacttga ccagcttcaa
    18001 caacaaaatg gcaaagcgat agatactcgc cccagcgcgt tttataacgg ctggccacaa
    18061 accttaaatg gcccttctgg tcatgaacct gccgccttaa acctctctgc cagctggctc
    18121 gacaaaatga gcaccgaaca gctcaacgcg tggatcaagc aacataacct gaaagccgat
    18181 gccccggtgg cgctgtacgg taatgacaaa gatgtcgacg ccgtcaaaac gcgactgcaa
    18241 aaagcaggtt ttacgcatat ctccattctg agtgacgcgc taagcgaacc ttcccgtcta
    18301 caaaaactgc cgcactttga gcagctggtt tatccgcaat ggctgcacga cctgcaacaa
    18361 ggtaaagagg ttacggcgaa acctgccggt gactggaaag tcattgaagc ggcctggggc
    18421 gctcctaagc tttaccttat cagccatatt cccggtgctg actacatcga taccaacgaa
    18481 gtggaaagcg aaccgctgtg gaacaaagtt tctgatgaac aactaaaagc gatgctggca
    18541 aaacacggca ttcgccatga caccacggtc attctgtacg ggcgtgacgt atacgctgga
    18601 gcgcgtgtgg cgcaaattat gctctacgca ggcgtgaaag atgtgcgtct gctggatggc
    18661 ggctggcaaa cctggtccga cgcgggactg cctgttgagc gaggaacgcc accgaaagtg
    18721 aaagcggaac cggatttcgg cgtgaagatc ccggcacaac cgcagttgat gctcgatatg
    18781 gaacaggccc gtggactgct gcatcgccag gatgcatcgc tggtgagcat tcgttcgtgg
    18841 ccagaattta tcggtacgac cagcggttac agctatatta aaccaaaagg tgaaatagcc
    18901 ggagcacgtt ggggacacgc tggtagcgac tcgacgcata tggaagattt tcataacccg
    18961 gatggcacca tgcgcagcgc cgatgatatt accgctatgt ggaaagcgtg gaatatcaaa
    19021 ccagagcagc aagtttcatt ctactgcggc accggctggc gcgcgtccga aacctttatg
    19081 tacgcacgcg ccatgggttg gaagaatgtt tccgtgtatg acggcggctg gtacgaatgg
    19141 agcagcgatc caaaaaatcc ggtagcaacc ggtgaacgcg gcccggacag tagcaaataa
    19201 cattgcaatt actgacgctg gagcgacttc agcgtcagat acccactcca cacccgcgta
    19261 aaggtcgtca tccagcacaa cgcaccgaag atccacgcaa accatgcagg gaataaacat
    19321 cccagcacaa acagtaagat cgtttctgtg ccttcggtta acccgcccag ataataaaac
    19381 gatttatgcg cataaccggg attatcaatc tgatgtttcg ccgccagcgc agcaaaagcg
    19441 agaaaactgc tgcctgtgcc gataaacgca aacagcagcc agccgcctgc cagggcgttt
    19501 tgctgcggtg cggcgaggat aaacccaaaa ggtaccagcg cgtaaaaaag aaaatcgaga
    19561 gaaatatcaa gaaagccgcc cgcgtcggta agccctctgc gccgcgccag cgcaccgtcc
    19621 agaccatcaa gcagcctgtt caacaaaatg acgacaagcg ccgccagata ccagcccagc
    19681 gccagaaaag gcagtgccag cacgccgatg gcaaatccca ctaatgttaa accatctggc
    19741 gtaatgcccg gtttatcaag aacccgcacg cactgatgca acaacggttt aatccgggga
    19801 tgaagatggc ggtctagcac aggcactcct taaatataaa gcctttctga ttgagcaaca
    19861 gtgcggatat tatggcattt ttcgcttatc tgcccgtgtg taatttatga aaatgattga
    19921 agttgttgcc gccatcattg aacgtgatgg caaaatttta ctcgcgcaac gccccgccca
    19981 gagcgatcag gcgggattat gggagtttgc cggtggtaaa gtcgagccgg atgaaagcca
    20041 gcggcaggcg ctggtgcgtg agttacgcga agaactgggc atcgaagcaa ctgtgggtga
    20101 gtatgttacc agccatcagc gagaagtttc gggccggatt atccatcttc atgcctggca
    20161 cgtacccgac ttccacggga cgttacaggc acatgaacat caggcgctgg tctggtgctc
    20221 acctgaagag gcgctgcaat atccgctggc ccctgctgac attccattat tagaggcgtt
    20281 tatggcttta cgcgccgcca gaccagcgga ttagtgctaa gggttttgtc atcccgctgg
    20341 cattgcagca gtattccctc ggctttaatt accgcgcctt cagaataatt tttatcctga
    20401 taaacgcagc actgagtaca gggctgcgct gactgcccgc ctgaactgaa cacttctggc
    20461 ggtacgttta cctccacgtc cggacgataa tgcgggttag ccagtgcaat taatggaaat
    20521 gctaatacta cggcgaacaa tgctcgactc acagggaact ccttaacgtt attgtctctg
    20581 ctactgataa cggtagccgg gtggcaaaac tttagcgtct gagttatcgc atttggttat
    20641 gagattactc tcgttattaa tttgctttcc tgggtcattt ttttcttgct taccgtcaca
    20701 ttcttgatgg tatagtcgaa aactgcaaaa gcacatcaca taaacaacat aagcacaatc
    20761 gtattaatat ataagggttt tatatctatg gatcagacat attctctgga gtcattcctc
    20821 aaccatgtcc aaaagcgcga cccgaatcaa accgagttcg cgcaagccgt tcgtgaagta
    20881 atgaccacac tctggccttt tcttgaacaa aatccaaaat atcgccagat gtcattactg
    20941 gagcgtctgg ttgaaccgga gcgcgtgatc cagtttcgcg tggtatgggt tgatgatcgc
    21001 aatcaggtac aggtcaaccg tgcatggcgt gtgcagttca gctctgccat cggcccgtac
    21061 aaaggcggta tgcgcttcca tccgtcagtt aacctctcca ttcttaaatt cctcggcttc
    21121 gaacaaacct tcaaaaatgc cctgactacc ctgccgatgg gcggtggtaa aggcggcagc
    21181 gatttcgatc cgaaaggaaa aagcgaaggt gaagtgatgc gtttttgcca ggcgctgatg
    21241 actgaactgt atcgccacct gggcgcggat accgacgttc cggcaggtga tatcggggtt
    21301 ggtggtcgtg aagtcggctt tatggcgggg atgatgaaaa agctctccaa caataccgcc
    21361 tgcgtcttca ccggtaaggg cctttcattt ggcggcagtc ttattcgccc ggaagctacc
    21421 ggctacggtc tggtttattt cacagaagca atgctaaaac gccacggtat gggttttgaa
    21481 gggatgcgcg tttccgtttc tggctccggc aacgtcgccc agtacgctat cgaaaaagcg
    21541 atggaatttg gtgctcgtgt gatcactgcg tcagactcca gcggcactgt agttgatgaa
    21601 agcggattca cgaaagagaa actggcacgt cttatcgaaa tcaaagccag ccgcgatggt
    21661 cgagtggcag attatgccaa agaatttggt ctggtctatc tcgaaggcca acagccgtgg
    21721 tctgtaccgg ttgatatcgc cctgccttgc gccacccaga acgaactgga tgttgacgcc
    21781 gcgcatcagc ttatcgccaa tggcgttaaa gccgtcgccg aaggggcaaa tatgccgacc
    21841 accatcgaag cgactgaact gttccagcag gcaggcgtac tgtttgcacc gggtaaagcg
    21901 gctaatgctg gcggtgtagc aacgtcaggc cttgaaatgg cgcaaaacgc tgcgcgcctg
    21961 ggctggaaag ccgagaaagt tgacgcacgt ttgcatcaca tcatgctgga tatccaccat
    22021 gcctgtgttg agcatggtgg tgaaggtgag caaaccaact acgtgcaggg cgcgaacatt
    22081 gccggttttg tgaaggttgc cgatgcgatg ctggcgcagg gtgtgattta agttgtaaat
    22141 gcctgatggc gctacgctta tcaggcctac aaatgggcac aattcattgc agttacgctc
    22201 taatgtaggc cgggcaagcg cagcgccccc ggcaaaattt caggcgttta tgagtattta
    22261 acggatgatg ctccccacgg aacatttctt atgggccaac ggcatttctt actgtagtgc
    22321 tcccaaaact gcttatcgta acgataacac gcttcaagtt cagcatccgt taactttctg
    22381 cgatagcagc agatatgcca gtaaagaaat cccattagac tatttttttg ataatcttct
    22441 tcgctttcga acaactcgtg cgcctttcga gaggcaagca ttatataatg ccaggccagt
    22501 tcttcttcaa ttgtcccgtt ttgaaaagct gtgctcgata tcgagatcat ccatgataat
    22561 tccgccgccc atattagctt cgccgaggat ttaccggagc tatgattagc gcaatcagag
    22621 atatagtctg agggaaaaac agcaaattta ttcaacaagg cgataacctg ctctggggct
    22681 tcctccatgt tcgctttaaa ggtattggct ccatggtcgc cagaaagaaa atgctccatt
    22741 aaggcacaat aactttcgct atcttcgata ccccattgat cctctaaaga ctcgcgtctt
    22801 ttacttatga tatcgatcga gtcaaaggga agcacatgat attggaaggt atctttgcca
    22861 ggttcaggct ttcgcggcca gaactccagc gtttcagacc attgcttatg atagaaccga
    22921 taaggtgcga tcaattgtag cgcctgtaac ttctcgatac tgagcggctc aataccttta
    22981 gcctgataat aatgcagttg ttcttttttt gctttaaaac cggcccgaac aataagcccc
    23041 atcataatta atagataaag aaaagagcat cccgcagtaa tcaggcctct ttcattcaaa
    23101 ccgttggatg ttatcgctgc gaatacaaac attacagcga caacacatgt taaatacccc
    23161 ccccacttac aaagcagcat ggccttattt tctttaatca tccgttcaaa attactatta
    23221 aatatttccc agccattaaa agaatacttc tcgctcccag gatggttttg taataaaact
    23281 tttttcatca cattccctgt tattacatac tttagaggcg ataattttat ccatgcaaaa
    23341 aaatatccaa cgaattttct tgatccgctg gatatttcag aatatgactc gaatagcacg
    23401 aaagattcgc tcgcttacgc tatcgccccg cttccggctt catctgctgg cggacttttt
    23461 tttcgcacta cgtttacgcg gtgcagcctt tttcttatca gcactgccac cactgcccgg
    23521 agccacaatg ccgcgaaact gccgcaccgg agtacgtttg gcttgatcaa taagctgata
    23581 tagcgtcccc accagcggct gcataaagtc ctgatagcga cactgctttt cgctgatttg
    23641 cgtcagcacc gattcccagt gcgcggtcat gtccggtcgc gtagccattt ctggcagcga
    23701 atggaataac gcttttccgg cgtcggtgga gtggatatag cgcccttttt tggtgaggaa
    23761 accacgcttg aacaacagtt caataatccc ggcacgcgtt gcttctgtcc ccagaccatc
    23821 ggtcgcacga aggatctttt tcagatcttt gtcctgcaca aagcgcgcaa tcccggtcat
    23881 cgccgaaagc agtgttgcat cggtaaaatg gcgcggcggc tgggtttgcc gctctaccac
    23941 ttcacctttt tcacacagta actcatcgcc tttcgccacc acaggcaatg gcgtgccgtc
    24001 gttttcttca tcgcgctctt tgctgcctaa cagcgcgcgc caacctgctt cagcaagaaa
    24061 acgcgcttta gcgacaaatt tgcctttggc aatgtccagt tcgataacac acttgcggaa
    24121 caccgcatcc gggcagaact gcatcagata ctgacgggca atcaggttat agaccttcgc
    24181 ttcgttctcc gtcaggttga tcgcagaact ccgtgcggtc ggaatgatgg cgtggtgcgc
    24241 atcgaccttt ttgtcatccc aacagcggtt gcgtatatct ggatctacca ctggctgcgg
    24301 caacagatct ggtgcatgaa cgctgatggc attcatcacc gcgtggcgtc cggcaaaatg
    24361 ttcttctggc aaatagcgac aatcggaacg cggataagtg attaacttgt gtgtttcgta
    24421 cagcttctgg cagatatcaa gcacgttctg cgcgctcaga ccaaagcgtt ttgccgcttc
    24481 aatctgcaac gccgaaagcg aaaacggcag cggcgcggat tctgattccc gtttatcgtt
    24541 atagctggtg acaatagccg gttgaccact aatgcggtta accacatgct ccgccagtgg
    24601 acgatgtaac aagcgccctt cttcatcctg gtacggttca cacgcttcgc tcggttgcca
    24661 gatagcggta aaccgctcat cggcaggtgt cacgatatgc gctttgactt caaagaagtc
    24721 tttcgccacg aagttttcaa tctcttcatc gcggcgcacc accagcccga gcacgggtgt
    24781 ctgcacgcgt cccacggaaa gtacgccctg ataaccggca ttgcgaccga gaatggtata
    24841 cgcacgggtc atattgatgc cgtacagcca gtcggcacgc gctcgcgcca gcgcagaaac
    24901 gcacaatggc acaaactcac tgttggaacg aagacggtcg atcgcccgct caaccgcctg
    24961 cgggttcagg tcgtttatca agcaacgctg cacctgctgg cgcttttccg gtgccagttg
    25021 cagatagtcc agcacttcat ccaccagcaa ttgcccttca cgatccgggt ccccggcgtg
    25081 aacgatttcg ctggcttcat gcaggaagcg tttgatgacg ttaagttgtt tggtcacgga
    25141 gggtcggggc tgtaattgcc acttttccgg gacaatcggc aaatccgcaa gattccagcg
    25201 cgcatagcgg ctgtcgtagg cgtctggctg cgcctgctca agcaggtgac cgatacacca
    25261 agtcaccacc tgaccattac cgcactcgat aaagccatcg cctttacggt gcggtttggg
    25321 caggacatca gcaatggcgc gcgccagact cggtttttcg gcaataaaca accgcattga
    25381 attaacggat ctcaaccatg gcacgaccgc cgcgcgctgg caccagttcg ccaatcgccg
    25441 tcagttcaat gccaaactcg gcggctgtag ttttaacctc attttctgct tccggcatga
    25501 ccgccagcag caaaccgcca gaagtttgcg gatcgcacag cagatcgcgc acttcacgcg
    25561 gcatttcacc catcagatga ccgtagctgg caaagttacg ttcggtgccg ccaggcactg
    25621 cgcccaactt aatgtactct tcaacccccg gcaattttgg gatcgcgtca tagtcaacgc
    25681 gcgcctgcac acctgcgccc tgacacattt cgctcaaatg gcccagcaga ccaaaaccag
    25741 taacgtcggt catcgctttt acgccttcga tgttggcaaa ggacgcacct gcgatgttca
    25801 tccggcacat cacttccgtc gccagtccct gatgttctgg tttcaacagt gattttttct
    25861 cagccgtggt aagaacgccg atccccagcg gtttcgtcag gaacagtttg catccggctt
    25921 gtgcggtact gtttttcttc acccgctcgg tcggtacgat ccccgttacc gccagaccaa
    25981 aaatcggctc cggcgcatcg atggagtgac cgccagccag cgcaataccc gcctgacggc
    26041 atgcatagcg tccaccttcg gttacttcgc gggcaatttc tggggaaagt ttgttaatcg
    26101 gccagccgag gatcgcaatc gccataatcg gtttgccccc catcgcgaag atatcgctga
    26161 tggcgttagt cgccgcaatg cggccaaaat cgaaaggatt atcaacgatc ggcataaaga
    26221 agtcggtggt actgataacg ctggtgccat tgcccagatc gtacaccgcc gcatcgtcgc
    26281 gggtctcatt acccacaagc aaattcggat caacaaactt cgcctgctca ctgtgcagga
    26341 tggtttccaa cacttttggg gaaattttac agccgcaacc agctccgtgg ctgtattggg
    26401 tcaaacgaat cgagttctcg ctcatggaca tctcctgtca atgcaatccg ggtatggtaa
    26461 ccctcattcc gtgaagtgat aagtgagagt gtctgaattc ctgcgccttt gctcacaatc
    26521 cagacagttt cgcgacaatt atcagaaata agttacaaac ggcgtcgggt ccgggacgtt
    26581 aatcgacgta gatgctttca actgcggcgt gccgaggtag agaaaaccga caattttatc
    26641 ctgctcacgg caaccgaatg cttcacgcac taccggactt tcagttaatg cgccactgcg
    26701 ccagatgccg ccaaatccct gggcaacggc tgccatttgc atcgccatga ccgcgcatcc
    26761 ggcagacatt tcctgttccc atcgcgggac tttatgattt tcttcgcatt tcgccaccac
    26821 cgtgatgatg agcggtgcgc ggaacggcgc attacgggct ttgtcgatag ctttgtcatc
    26881 actaccggca gcaatcgccc cctgttccag tacggcgctg aaacgctcgc gcccttcccc
    26941 ttcaatcaca aaaaaatgcc acggttgcat ggacttatgg tccggcgcac gcatacccgc
    27001 acgcaggatg ttttgcagtt gttcacccgt tggcgcgggt tcagccaggc gggaggcgct
    27061 acggcgattg atcaatagtt cgagtgcatc catttgatta actcctgtcg tgatatttat
    27121 tcacaaaatt aacacaagag tggattttgt tacagcacag tccgcaattc ctgctgacaa
    27181 gtgccggttg ggtcattacg ataaccacat ctattgcgcc tgtgacaggt gtgaccttaa
    27241 gttgggagaa tacatgcgaa ccctttggcg atttattgcc ggatttttta aatggacgtg
    27301 gcgtctgctg aatttcgtcc gtgaaatggt acttaacctg ttctttattt tcctcgtact
    27361 ggttggtgtg gggatctgga tgcaggtcag tggtggtgat tcgaaagaaa cggccagtcg
    27421 tggcgcactg ctgctggaca tttctggtgt gatcgtcgat aaacccgaca gttctcagcg
    27481 gtttagtaaa ttaagccgcc agctgcttgg tgccagttcc gatcgtctgc aggaaaactc
    27541 actgtttgat atcgtcaaca ccattcgcca ggcgaaggac gaccgcaata tcaccggtat
    27601 cgtgatggat ctgaaaaact tcgcaggcgg cgaccagccg tctatgcagt acatcggcaa
    27661 agccctgaaa gagtttcgtg acagcgggaa accggtttat gccgttggcg agaactacag
    27721 ccaggggcaa tattatctcg ccagtttcgc caataaaatt tggctgtcac cgcaaggcgt
    27781 ggtggatttg cacggttttg ctaccaacgg tctgtactac aaatcgttgc tggataagct
    27841 gaaagtttcc acccatgtgt tccgcgtggg tacgtataaa tctgccgttg aaccgtttat
    27901 tcgtgatgac atgtcaccgg cagcccgcga agctgacagc cgctggattg gtgagctgtg
    27961 gcaaaactat ctgaatactg ttgccgctaa ccggcagatc cctgctcagc aggtattccc
    28021 tggcgcgcaa gggttgcttg agggtttaac caaaaccggt ggcgataccg cgaaatatgc
    28081 actggaaaac aagctggtcg atgcactggc atcgagtgcc gaaatcgaaa aagctctgac
    28141 caaagagttc ggctggagta agactgataa aaattatcgc gccatcagtt attacgatta
    28201 cgcattgaaa acgccggcag ataccggtga cagcatcggt gtcgtcttcg ctaatggcgc
    28261 aattatggat ggcgaggaaa ctcaggggaa tgttggcggt gataccactg cggcacaaat
    28321 ccgcgacgct cgccttgacc cgaaagtgaa agcgattgtc ctgcgtgtta atagcccagg
    28381 cggcagcgtt accgcgtctg aagtgattcg cgctgaactg gcagcagccc gggcagcggg
    28441 taagcctgtg gttgtatcga tgggcggcat ggcggcatct ggtggttact ggatttccac
    28501 gccagctaat tacattgtgg ctaaccccag caccctgacc ggttctatcg gtatcttcgg
    28561 cgtgatcacc accgtagaaa atagtctgga ttcgattggt gttcatactg atggtgtctc
    28621 aacttcaccg ctggcggatg tttctatcac cagggcactg ccgccggaag cgcagcagat
    28681 gatgcaatta agcattgaga atggctataa acgctttatc acgctggttg ctgatgcgcg
    28741 tcattcgacg ccggagcaaa ttgataaaat cgcccagggc cacgtctgga ccggtcagga
    28801 tgcaaaagct aacgggctgg tcgatagtct cggggatttc gatgatgcgg ttgccaaagc
    28861 agcagagctc gcaaaagtga aacagtggca tctggaatac tacgttgatg aaccgacctt
    28921 cttcgacaaa gtgatggaca acatgtctgg ttctgtccgg gcaatgttgc cagatgcgtt
    28981 ccaggccatg ttacctgcac cgcttgcctc ggtagcctct accgttaaaa gtgaaagcga
    29041 caagctggcc gcgtttaacg acccacaaaa ccgttatgcg ttttgcctga cctgcgccaa
    29101 cgtgcgttaa gtcttgtact gagtggccga cagatcgtcg gccacattat tttttacctc
    29161 gacgaatcct cttcccgctg tctcgcccca tatttcctta tccgcatagt atcaggtgcg
    29221 ctcctcctgc ctcacgtata tacttttgct ctttcgatat cattcatatc aatatcatgc
    29281 aaaagaaatc aatttacgtc gcctacacgg gcgggaccat cgggatgcag cgttccgagc
    29341 agggttatat tccggtgtca ggtcatctac aacgccaact ggcgctgatg ccggaattcc
    29401 atcgcccgga gatgccagat ttcaccattc atgaatatac gccgttgatg gattcttctg
    29461 atatgacgcc ggatgactgg cagcatattg ctgaagatat taaagcgcac tatgacgact
    29521 atgatggttt tgtaattcta cacggtaccg acacgatggc gtataccgcc tctgcgctgt
    29581 cgttcatgct cgagaatctc ggtaaaccgg tcattgtgac agggtcacaa atcccgctag
    29641 ctgagttacg ctctgacgga caaattaatc tgctgaatgc gttgtacgtt gcggcgaatt
    29701 atccgatcaa cgaagtaacg ctctttttca ataaccgatt gtatcgcggc aaccgcacta
    29761 ccaaagccca tgccgatggt tttgatgcgt ttgcctctcc aaaccttcct ccgttactgg
    29821 aagcaggtat ccatattcgt cgtttgaata cgccacccgc cccgcacggt gaaggggaat
    29881 taatcgttca tccaatcacg ccacaaccaa ttggcgtagt gacgatttat ccggggattt
    29941 ctgctgatgt cgtgcgcaat tttctgcgcc aaccggtgaa agcattgatt ctgcgctcct
    30001 atggcgtggg taatgcgcca caaaacaaag ccttcctgca ggaattacaa gaagccagcg
    30061 atcgcggtat tgtggtggtc aacctgacac aatgtatgtc cggtaaagtg aatatgggtg
    30121 gttatgccac cggtaacgcc ctcgcccatg ccggcgttat tggcggtgca gatatgactg
    30181 tagaagccac gctaaccaaa ctgcattacc tgctgagcca ggaactggat actgaaacca
    30241 ttcgcaaggc catgagccaa aacctgcgcg gcgaactgac gccggatgat taaggagact
    30301 gcaatgcccc atcgcgccct gttactggtc gatttacaaa atgatttctg tgctggtggc
    30361 gcgctcgccg taccggaagg tgacagtacg gtggaggttg ctaaccgcct tattgactgg
    30421 tgccagtcgc gcggagaagc ggttatcgcc agcctggact ggcatccggc gaatcacggc
    30481 agttttgcca gccagcacgg tgtagagcct tatacgccag gccaactcga cggtttgcca
    30541 caaaccttct ggccagatca ctgtgtgcag aacagtgaag gcgcacaatt acatccgtta
    30601 ctgaaccaaa aagcgatcgc agcggtgttc cataaaggcg aaaatccttt agttgacagt
    30661 tacagtgcct tttttgataa cggccgtcgg cagaaaacct ctctcgatga ctggttacgc
    30721 gatcatgaaa tcgatgaatt gatcgttatg ggcctggcta ctgactattg cgtgaagttt
    30781 accgtgctgg acgcgttaca gttaggttat aaggtgaacg tgattaccga tggttgtcgt
    30841 ggcgtgaata tccagcccca ggacagtgca cacgcgttta tggagatgtc agcagctggg
    30901 gcaacgctat atacgctggc agactgggaa gagacacagg ggtaatttta cgctggccta
    30961 caattctgta ctggcattgt aggccaaata aaacacgtca gtggtacatc tggcaattga
    31021 tgccatcaac gaaagattta attcacctca gaaatctctt ccagcgacac cttccgcgtt
    31081 tcgatgccaa aaatggagag aaccagcgca cagagcacta acatgacacc aagcaccata
    31141 aacaccgtga tcgacccata atgtgttaat aatgccgcaa cgccataggg cgtgaaaact
    31201 gcgacgatcc gtccgacggc attaacgaaa cccgaaccgc gcaggcgtaa atgcgttggc
    31261 caaagctccg ggatataaac cgctgacgcg aagcaaacat acatgtataa aaagaagatc
    31321 atcaccagac catagattaa aatcgcccac tctgtagtct gaattgaata gatatagcct
    31381 aacacggcaa taataataag taaggcggag ccaaataatc gacgaggaaa atgatcaata
    31441 attaatgccg caataaatat tcctaccgga gcaccaatca taataacagc ggtcattaat
    31501 attgatttat cgacatcaat cccggagtta acaaatatgg tcggtatcca tacggtaatg
    31561 gtataaagcg aaatgttcat tgcaattaaa acagttatcg caactaatgt acgtcgtaac
    31621 atttcccctt taaacaggag ccagaaggta cccttgatta ccttaacctt gctgttgctc
    31681 tggttcaaag taagctgggg taaacgaata ctcttctccc tttcaatttg ctgctctact
    31741 tcacgaagtt ggctttctgc accggcgatt tgccctttcc ctgccagcca tcgtggcgac
    31801 tcgataaagt atttacctga gagaaaccag gctaacagta tgccaatacc acccagaaga
    31861 aacattattc gccaactaaa aaaagcgata accaccacgc ctatcgccgc agacagcatg
    31921 ggcgaccagt taccaacaaa tgagagccgc gcggaccatt ttccacgcac cgtcgcgggg
    31981 ataaactccg taaatgaggc atagccaacc ataatcagcg cccccattcc tgttcccatc
    32041 aggaagcgaa agaaaatgag ccagtacata tcagggacaa aagcggcccc tgttgcagca
    32101 ataccgacga tgagaagatt tatgcgaaac gccctgcgcc gcccaaagta gtcaccaata
    32161 aacccaccag taagtgagcc gatgaaataa ccaaacatta atgccgaggt aaaggcggca
    32221 ttgaggaaat tatttgacca tccattgctt accagctttg ctaagacgac attacctgag
    32281 taactcaaaa accccgttaa taacagacta aagcttatta taccaaatat acgataatga
    32341 aaccgggcca aaggcaagcg gtccagtctt gcgccaattt gatcatattg ttccattagg
    32401 tgcctcggat ttgctaaaaa ggtagcaatt gcggcaaatt cacttgccgc atctttatca
    32461 tcattttatt tcaaggcgca ggaacaaata atattgacaa cgtgtgctta aattaccaca
    32521 ccagctggat attattgtct ttgcaaaatg ctatccactc tgcacccggt gatttatcag
    32581 ttattatgta attaatatga ctaaaatcag ctaactggac aaaagctttg cgatcaaact
    32641 tagagtgatc aaccaataac gcaacttctg tcgcctgacg gatcattgtc tttttgattt
    32701 cagcttccgc ttcattagag tccagcgcgc cgctgttaat atcaagacct ttacaactca
    32761 ttaccatgat atcgacatgg tagcggctga tgatctcttt agtaattctt ccttgcaggg
    32821 aaagtgtatt tttgtttagt tccccccccg ttgaaacgac tttaatttct gactgagcca
    32881 gaacatgaat cgcttctgcc gagttggtta gcaacgtcag gtcactacgg tcctgtaaca
    32941 atttgagcaa ttccattacg gcactactgg aatcggctgc catggtggtt ttattgtcga
    33001 taaagggtaa tgccttgcgt gcaataagct gcttctcttc ataaaacgat gaagcgcgct
    33061 tataaaaatg gatattctcc gtcaacatcg ctgtatttaa aacagcccca ccataggttc
    33121 tggtcaaaaa gccttcatct tccagcttct caagatcgcg gcgaatggtt tcttcggtta
    33181 cctgaaaaat cccactcaaa tttgagactg tcaccttttt atcgttggca accatttgct
    33241 taattgcctg aatcctgtct tttgccgcca cgattacacc cctgtatctt tttacatcac
    33301 attagcgcga ttatcgcata accgatgttt actttcaaaa taacctgttt gaatcacaga
    33361 ttttcatcac agttttcaca gaaacagagg tgaatcgtgt tgagtattta acgctccagg
    33421 gcctctgcca tttccctcat caatgttgcg tctgcatccg ataagttgat attcagtgcc
    33481 gcgacatttt cgcgtacctg ttccggtgca gtagccccac taagaatgga gattaaatca
    33541 ctctgtttta atatccacgc cagtgccaga gtgggaattg tgcactgata acgagcacaa
    33601 agtggctgcc actgttcaag catatcaatc actttcagca tgttttcacg ctggaaccag
    33661 actttatttg cccgagcgcc gcccggaacg taatcacgag tgatggtgcc ggtcaacaat
    33721 ccctgctcta gcggggaata aacctgaacc acaatgccat tatcacgaca tagtggcagc
    33781 agttcgtttt ccattgcccg gtcgaggata ctgtatttcg cctgaataat atccagttca
    33841 ccatattgca gatactcgcg gatatggtca gcatcgacgt tagcagcgcc tatagcgcga
    33901 attttccctt cagactttaa ctcattaagc actgcgacag tttcagcgat cggcgtaaaa
    33961 aatggcggca ccgactgcca gtgcgtcatg tagatatcga tgtaatcaat acccagacgt
    34021 tgcaagctcg ctgctacctc ttcgcggata gattccgggg aaaggttttt atacaactgc
    34081 cgatcgccaa ctttgttgaa taaacttcct tttcgttccc agacaatgcc gcatttggtt
    34141 tctactacaa cctgttcacg gggcagtttt tttaacgcct gaccgacgat aacttcacta
    34201 ttgccaaagt tatatcctgg cgcagtatca atcagattaa tgccacaacg atgggcttca
    34261 agaatcgtat caatacatat ttgccgatcg agatcgccat tccatgcagg accgccgcca
    34321 atggcccatg tccccaaccc cattcgcgaa agcgtaatat ccgttgtgcc taaaggtatc
    34381 tttttcattt gccttcatta tccttcgtat tcttccagca attgttcgac cagctttctg
    34441 ttttttacgc cggtggtggc accgacgctt agaaccgaga tagccgccgt tgcattggca
    34501 aagcgtgcgc attcacgcag atttttgcct tctaacagtg ccgcaataaa acctgaagca
    34561 aagttatcgc ccgcgccaat ggtgtcgatg gcggttattc ctgcgaccgc cggcaccttc
    34621 atcgtcatgt caccacgctt gataaagcag ccgtctttac ccgttttaat caccaccgtt
    34681 tttacgccgc acgcaagaaa gcagtcagca atttcatcca gtgtctcttt cccggtgagt
    34741 aattttgcct cggcaaaatt aggaaacaga taatcgacat aactcaatgc ttcgcaaata
    34801 tcatccagcg tttcattcaa ccgcggtttg atcatatcgg cacagataat catctgccgg
    34861 gctttagctt gcgtaaaaat ttctgttagc gctttaccat ccaatagtgg actgttgaaa
    34921 atactggcca gtgataataa tttcgcctga gaaaaccgcg caaaatcaac atcgtcaata
    34981 ttcaatttcc acagactgcc attacggttt gtaacaaacg tccgctcgcc atcctccgtc
    35041 accagtccaa cgttaataga ggtatctatg ctgacgtcct gcttcaggct ttgaatatca
    35101 atattatctt tgcggcaatg gtcgagaata aattgccctg cggcatcttt accaatacga
    35161 ctcattaatg ctgtgcgatg gcccagacga gaaataattg ttgcttcatt tattgcatca
    35221 cccccggtgg tcattgcgat tctttcaaga ggataagaat ccacatcaaa gatattttta
    35281 ctgaccggtt gcaatggaat atcaacaata gcggcaccta tacaaataac gtcgagatta
    35341 tccatatcat tattccgctt tgccatcaga accgaacagt ttaatttttt ccaatgcccg
    35401 ttcttttaca gctttacgta cttcacgttc taaatgcagg aaaggctgat cctggttttc
    35461 tttcactgca accattgcgg cctgacagag ctccgtatga atgttgattt tcgcgatacc
    35521 caacgaaatt gcagttttaa tgtcggcatc actgatcccc gatgcaccgt gcaaaaccag
    35581 cggaacagaa acggcatcgc gtacgcgttt gacgacctca aagttcaatt gtggctctga
    35641 cgtataaacc ccatgctggt tgccgatggc gacagccaat gaatcacagc ccgtacgttc
    35701 aacaaattca gccgcctggt caggatcggt atagtgatag cccgccagcg cttcctcata
    35761 aacggtttca ttaccgacat gccctaattc cgcctctacc ggaataccca gcggatggaa
    35821 gaaatcgaca gcctctttgg ttaaacgaat attttcttcg aaatcaaacg cggaagcatc
    35881 acgcattaat gaattcatac catgagtcca ggcgttatga ataatctcca tactccgacc
    35941 atgatcccaa tgagttatta ccggcaccgt tgctttttgt gccattgata ccatcatgtg
    36001 agagaaatct tcaaatgagg tgttaccgac aaaacctgta ccaaaagaaa taataaccgg
    36061 ggatttcgct tcttcggctg cgtcgataac gcccatcaac atttctgcat tccatacgtt
    36121 aaaatgggca attgcataat gtttatttgt ggcatcgttt tcccaatatc tgatatctgc
    36181 gagcatgttc tgttctcccg tattaattaa tcgctaattt taataacgcc tttaataatg
    36241 tcgcgtttgt tgttaactga ctcttcaaat gcctgttgta catcccgata atcgtaaata
    36301 tgcgtcacca tcgatttcac atcgaatcgc cctgaagaaa tagcttcaat cgtgaccgga
    36361 taacgattgg catagcggaa taccgtctgg atagtgactt cgcgattgat tttgaggaaa
    36421 ttgattgccg aatcgccggg tacagtacca acaatcataa ttttaccgcc gcgcattacc
    36481 agataaggtg cctgtttaac ggtgaccgca gaacccgctg tttcgaaaac aatatctgcg
    36541 cccatgtctt cggtaaattg ctgacagcgt gcaatagtgt cttcttttgt gccgttaata
    36601 accactgtcg caccaagttg ttccgccatt atcagacgtt tttccagcac atcaacgacg
    36661 gcaatttccg ttgcgcccag gcatttgcac gcttgcaacg tcatcaaacc aatacaacct
    36721 gctcccagaa taattatctt cttacccggt ttaacatctg ccagcatcgc ggcatgcatc
    36781 ccgactgcgg caggctccac cagcgcccct tccatcgtgt ccatattgtc tggcagtttg
    36841 taagtaaagc tctccggatg acacagatag tgcgttaatg cgccgcggta gttgggttgt
    36901 gtcgccataa aatcaacgtc cgggcagatg ttatatttgc cttccagaca gtaacgacag
    36961 tgaccgcaag gaacgccagg ttcgatattt acccgatccc ccggtttaaa tttgcgcacg
    37021 cggcttccca cagccacaac cgtcccggcg cattcatgac ccaggccaat ttcttgattt
    37081 gggtctttag gcggaataaa cgggcctgat tcaaaaccat gtacatctga accacaaata
    37141 ccgacatatt ctactttaat caaaacttca tcttctttag gcactggtat ttctgctgaa
    37201 ataattttca ttgtgcccgg cacctgcaat attgcttttg aatttttcat tttgaatcca
    37261 cccgtattat ttattggcta ctgcatcaat acttaaactt tcaacggaga cacctttggt
    37321 ttcaattcca atggtggcga tagcaattgc gacaacaatt gaaacagccc ccagaagaat
    37381 aaagactccc gttacgccat aactactgag cagcactgca acggcataag gtgcggcaat
    37441 accactgatt cgccctaccg cattcgccag accggagcca cggagtttgg cctctgtcgg
    37501 ccagatttca gggacataca ctgccgaggc atagcaaacg tacatataga cgaaagtaat
    37561 caggaagaaa ccaatcaacg ttatgagcaa catactggtt tgcagtgaat agatatatcc
    37621 gagcaccgca atcaggatta atagccccac acccattgtt ttacgcggaa ttttatccat
    37681 caccagcatg gcaataaaaa taccaaatgg cgcgccaaac atactcatgg tatttaaaac
    37741 aatcgagtct tttaaattaa tcccctgggt catgaatatt gttggcaacc agttaattag
    37801 tgtgtactgc acaacgttca tggcaatcag cacacaagag cctaatatca cgcgtttcag
    37861 taatactcct gtcagtaagg ctgaatacgg caccgcttgt ggcgctttac cgtcatcagc
    37921 aataaccaca ggcggcaaag gtttacccgt ctggcgtatg acgccttctt ctatactccg
    37981 catgactttc tcggcttctt gataccgtcc gcgcgattcc agccagcgcg gggattcagg
    38041 aaagtagcgc caggccagcg ccgtagcgat aagcgacaat attgcaggga taagcagttg
    38101 tacccgccag ttccactctg cactaatcag cggcgtgagt cccatcgcaa tcaatgaaca
    38161 gagcggatat gaccaggcca ataaaggaaa cccgactgga ccacgttccg cgatttctac
    38221 cgggcatata ttcggtgaaa ccagcaaaca gtgtaaccag taaagctccc agcccaacgc
    38281 ccatcacaaa acggcaggca ataagaaaat ccatattcgg tgagaaagca ccgacaacca
    38341 tcgaggcaat atgaatggcc tcatagagga tgaacgcatt tctgcgaccg gttttgtcgc
    38401 caatgatgcc gcccaccagc gcaccgagaa acattccggc ggtcgtgatt gctgagaatg
    38461 tggctgtggt ggaattatct gtccagccca acgctttcag ctgcgcgagg atcaagccac
    38521 caacggcatt actccagcag acaagcaagc caaacgcgac gatggcaaac attgacgaat
    38581 gccagcggca atccggtaag cgatccagcc gtgcaccaca atgcggtttt gttatctgtt
    38641 ccatgcgata acgttccttc aggagagtag ttattcatca aagtcgtaag tcatgatcac
    38701 tttgattgcg gttttatcga ccatcgcatc aaacccttcg cgccattgcg acaggccgat
    38761 acgatgcgtg atcatcggtt tgactttgat agcgccgctg gctaatagcc ggatagcgtt
    38821 acgccatgag gtggagtcat aggccatatg cccaatgatg cttttattcc aggcggtaat
    38881 gtcattaatc gagaaatcaa gaggtttgaa gcccattcca acgcgtacca cttccccatt
    38941 cgggcggagc atttcgatgg cttgtttcag tgcgatattg gcaccggagc attcaatcac
    39001 cagtcccaga ttatctttgc cacaaatttg ctggcagcgc gccaccacat cttcggtaga
    39061 accatttact actgccgtcg cccccagttc ttttgcaacc gggaagcgga ccgccacatc
    39121 ttcttgcaga ccaacgacga cgatatttac cgcccccata atccgcgcca tttgtacgga
    39181 aaacagcccg agtgggccag tgccgatgac gacaacatcc tgaccaggaa ggaatttcga
    39241 ttgctgcgcg atggatttat aggcattaca gatagggtca agtacggctg cgtcctcata
    39301 atcaacacca tcagggattt cccacaacgc atgacgatga attttgagaa tttcaccagg
    39361 aaccagacaa tatttggaaa aaccaccgcc ccaggtattg ttatccagac caaggtttac
    39421 tttttctgta caacacagaa aatcaccctg ttcacaggcc ggacaaacgc cgcaaacgtg
    39481 accgctgtta tccgacacga cgcgttgccc cactttccag tctttgactt tttcaccaac
    39541 ctgcgcaata caacctgcga actcatggcc gcggatagag ttaaactcat cagaaccgct
    39601 atcgacattg tagtgcttca tgtctgcgcc gcagattgcc gcggctttaa tttcaatcac
    39661 tacgtcttcc gggccacaaa tgggttgtgg gacatcaatc atcttgtagc cgccaaaggc
    39721 cttgccaaac cgagccagtg ctttcattgc gtcatcctct gtgttttgtt ttgcttagat
    39781 ggccatcata cttgcccaca tgatttttga ggtcaacatt aaatctgtaa aacacagata
    39841 atgatctgcg ttttacaact tagatcacaa ttacgcagaa aatggctggg tctgttacag
    39901 gttggtggaa ggcgggacgc aaaaagagca aattcgagag ggattaccgg ccaaataggt
    39961 ctgagactta agttcaggag aagtcattca ggcgtgaacg cgactgcaag agatcggact
    40021 gggacttagc caggtcgggt cgagataatt gagacgagtc gtggggtcat tgttggctat
    40081 ccccttcata gccgcggcaa aaaggggata aaccacttac ttaaacatag caataggctt
    40141 tgctgagata ccaaaatctt cttttaactg ctgtttgctc ttcatgacca tctgaccatt
    40201 tgtgtcaatt gtcatatgct gtgcttcggt attatggcgg gcttgccaca gcatcaccag
    40261 ttgcaggcaa ttctcttttt gttcctcggt caacgcaacg ccatcaggcc atttccccag
    40321 ttcaacggcg gtcgacaaac gctggtatac ctcaggcgtc atgctgttga taatgtcatc
    40381 aagattcata atttttccgc tcctgtggaa taagttgctg aatcgttttt tcaaccgttg
    40441 atttcttcgc cgttttcgcc atcggtaaag cgtaaagagg cagagttaac acaataacgt
    40501 tcgcccgttg gctgcggccc gtcagggaag acatgcccca gatgggcatc acagttaccg
    40561 caacgaattt ctatgcgctg cattccatgt gacaagtctt tgatataacg aatggattct
    40621 tcactcaccg gttcgtagaa actgggccaa ccacagccgg aatcatactt ggtttgggaa
    40681 tgaaacagcg gggcatcgca gatcaaacag tgatatacgc cgtcacgctt gttatgcagt
    40741 aaacgacccg taaatggcgg ctctgtccca tgattctgcg tcacgtaaaa ctgcatctcg
    40801 gacaaatttt ttttcagttc ttctgccgaa ggtttattag ccatttgctc acatctcact
    40861 ttaatcgtgc tcacattacg tgactgattc taacaaaaca ttaacactaa ctggcaaaat
    40921 tttgtcctaa acttgatctc gacgaaatgg ctgcacctaa atcgtgatga aaatcacatt
    40981 tttatcgtaa ttgcccttta aaattcgggg cgccgacccc atgtggtttc aagcccaaag
    41041 gaagagtgag gcgagtcagt cgcgtaatgc ttaggcacag gattgatttg tcgcaatgat
    41101 tgacacgatt ccgcttgacg ctgcgtaagg tttttgtaat tttacaggca accttttatt
    41161 cactaacaaa tagctggtgg aatatatgac tatcaaagta ggtatcaacg gttttggccg
    41221 tatcggtcgc attgttttcc gtgctgctca gaaacgttct gacatcgaga tcgttgcaat
    41281 caacgacctg ttagacgctg attacatggc atacatgctg aaatatgact ccactcacgg
    41341 ccgtttcgac ggtaccgttg aagtgaaaga cggtcatctg atcgttaacg gtaaaaaaat
    41401 ccgtgttacc gctgaacgtg atccggctaa cctgaaatgg gacgaagttg gtgttgacgt
    41461 tgtcgctgaa gcaactggtc tattcctgac tgacgaaact gctcgtaaac acatcaccgc
    41521 tggtgcgaag aaagtggtta tgactggtcc gtctaaagac aacactccga tgttcgttaa
    41581 aggcgctaac ttcgacaaat atgctggcca ggacatcgtt tccaacgctt cctgcaccac
    41641 caactgcctg gctccgctag ctaaagttat caacgataac ttcggcatca tcgaaggtct
    41701 gatgaccacc gttcacgcta ctaccgctac tcagaaaacc gttgatggcc cgtctcacaa
    41761 agactggcgc ggcggccgcg gcgcttccca gaacatcatc ccgtcctcta ccggtgctgc
    41821 taaagctgta ggtaaagtac tgccagaact gaatggcaaa ctgactggta tggcgttccg
    41881 cgttccgacc ccgaacgtat ccgtagttga cctgaccgtt cgtctggaaa aagctgcaac
    41941 ttacgagcag atcaaagctg ccgttaaagc tgctgctgaa ggcgaaatga aaggcgttct
    42001 gggctacacc gaagatgacg tagtatctac cgatttcaac ggcgaagttt gcacttccgt
    42061 gttcgatgct aaagctggta tcgctctgaa cgacaacttc gtgaaactgg tatcctggta
    42121 cgacaacgaa accggttact ccaacaaagt tctggacctg atcgctcaca tctccaaata
    42181 agttgagatg acactgtgat ctaaaaagag cgacttcggt cgctcttttt tttacctgat
    42241 aaaatgaagt taaaggactg cgtcatgatt aagaaaattt ttgcccttcc ggtcatcgaa
    42301 caaatctccc ctgtcctctc ccgtcgtaaa ctggatgaac tggacctcat tgtggtcgat
    42361 catccccagg taaaagcctc ttttgcatta cagggcgcac accttctctc gtggaaacct
    42421 gcgggtgaag aagaagttct gtggttgagc aacaacacac cgttcaaaaa tggcgtcgct
    42481 attcgcggtg gcgtaccggt ttgctggccg tggtttggtc cggcggcaca acaaggtctg
    42541 cctgcgcacg gttttgcccg caacctgccg tggacgctga aatcacatcg tgaagatgct
    42601 gatggcgtag cgctgacttt tgaattgaca caaagcgaag agacgaaaaa attctggccg
    42661 cacgacttta cgctgttagc gcatttccgc gtgggtaaaa cctgtgaaat cgatcttgaa
    42721 tcacatggcg aatttgaaac cacctctgcc ctgcatacct actttaacgt gggtgatatc
    42781 gctaaggtaa gcgtcagtgg gctgggcgat cgcttcattg ataaagtaaa tgacgcgaaa
    42841 gaagatgtac tgaccgacgg tattcagacc ttccctgacc gtaccgatcg cgtgtatctg
    42901 aatccgcaag attgcagcgt gattaatgat gaagcgctga atcgtattat cgccgtaggc
    42961 caccagcatc atctgaacgt tgtcggctgg aacccgggac cggcgctttc agttagcatg
    43021 ggcgacatgc cggatgatgg ctacaaaaca tttgtttgtg tagaaacggc ttacgcttca
    43081 gaaacgcaaa aagtgaccaa agagaaacct gcacatctgg cgcaatccat tcgcgttgcg
    43141 aaacgttaat ttgcgttaat gttgtgtgcc gggtgcgatg catccggcac gtatcaacat
    43201 cacaccatat ccagcgcagt ttttcctttt ggtgccggat acgctttatc cagcatggtt
    43261 aattccgctg acgaaagttc gacctcaagc acagccgcat tctgttggac atgggcaatc
    43321 gtggccgctt ttggaatcgc catcacaccc tgatggctga ttacccacgc caacaatact
    43381 tgtgccgcgc tgatattgtg agcatgtgca atttcgttga ctaccgcgtt ttttaacagt
    43441 ccattgcgca accgcccggc ctgggctaat ggactgtaag ccatcaccgg catctgctgt
    43501 tgctggcacc aggggagtag atcgtactca atccctcgtg aaccgagatg gtaaagcacc
    43561 tgattagtgg cacactgatt tccccccggc agctgccaga gttcctgcat atcagcatga
    43621 tcaaggttag aaacgcccca gcggcggatt tttccctggg cgatcaattt ttccatcgct
    43681 gccacagtct cttcaaaagc gaaactgcca gaccagtgta ataagtagag atcgagataa
    43741 tcagtattga gacggcgtaa actggcttcg catgcattta tcgctttttg cccgccagca
    43801 ttccacggat agactttaga gacgagaaag acgttatcgc gcagaccggt taatgcttcc
    43861 ccaaccacct tttcggcacc gccatcggca tacatttcgg cggtatcaat gagggttaaa
    43921 ccgagctcaa tgcccgcgcg tagtgcagca acttctgttt tgcgctgact ggcatcttcg
    43981 cccatatacc atgttccctg ccctatggct ggcagtgaga catcgccact aaattgaatc
    44041 attttttgtt gcattgtttc ctcccaggta ttgcaccacc gtaatgcaaa acagggcgtg
    44101 acgccctgtt tttatgcaca aaatgccctg gaaagatgca ttatcagaat ttgtaggtga
    44161 tcccggtaga aatcaggcca gtccaggatt tatccaccat cgggctgtca gtaacttcat
    44221 cagacagacg ggtatagcgc gcggtaccgt aaacactcca gtcgccgagg aagttgtagc
    44281 tggcgctcag ctccaggtaa gggctccagc tgtcgttcgg gttatagcca cgcagaccgc
    44341 tgcgagcgga ctctttgcgc gatacgccat aatagtattc gttctggttt tcgctgttcc
    44401 actgcacacc gatacccggg gtcacggtca ggccgccgtt ggtgtaacga tacaaccagg
    44461 ccatatccca gacgatgccg ttgctgttat ccagggtatc gccagccagg gtggtacgca
    44521 ggtaaccgta ctgggtaaag tgagcataag acagaccagc catcatggtg ctcttacggt
    44581 catccaggtg acgcatttgg tgatcgccac tgtctttcgc tttgaagtaa agcggcgacc
    44641 aataagcggt aattgaaagt ttgtccgttg cgtcattcca caggtagtaa ccaccaccta
    44701 agccacggaa ccagaagtta tcgccttcat agttgattac cggtactggg taaacatcgg
    44761 tatcgtaatc tttatatggg tgttcaacga cacctacgcc tgcgcccagg gaaaatttac
    44821 cttcagcgtg cgctacgcct gcagacgttg cgataagcac tccaagtgcc agaagtttga
    44881 gtttggtcac aattaatcat tccttaaaca aatgtttagc gggcgacaaa gtttacccgt
    44941 caatacatcg aaacccaacc tttttacgtt ttcatttttt aaagtaactg tttaattttc
    45001 ctgacgcgga tgacaccgtg cttacagcca aatgaattta gcgttactgg cgagccttgt
    45061 ctttacatta attatgcaaa atttatggat gagttgttga tatgccattg aaattaagaa
    45121 agccgtgcag gcaagttttc catttgccat ctacgcttaa ttttgaaggt gtatcaccgg
    45181 gcacgttgtt ctcatcgtcg ataaaatggc atgagagttg ctgtgtttta gcaagagacg
    45241 tcgttcagtt tacctcttcc gggagcctct actattcata tgaacggctc ttaacctgtg
    45301 ctaaaaaacg aaaggacggc ataccatgaa tatattcgat cactatcgcc agcgatatga
    45361 agctgccaag gacgaagagt tcacactgca ggagtttctt accacttgtc ggcaagatcg
    45421 cagtgcttat gccaacgcgg ctgagcggct attgatggct atcggtgagc ctgtcatggt
    45481 cgatacagcc caggaaccca gactttctcg actcttttct aaccgggtca ttgcacgtta
    45541 tccggcattt gaagagtttt acggcatgga agacgcgatt gaacagattg tctcttatct
    45601 gaaacacgcg gctcaggggc tggaagagaa gaaacaaatc ctgtatctgc tggggcctgt
    45661 gggtgggggt aaatcatcgc ttgctgagcg actgaaatca ttaatgcagc tcgtaccgat
    45721 ttatgtattg agcgccaacg gtgagcgtag cccggtcaac gatcatccgt tctgtctttt
    45781 caatccgcag gaagatgctc agattctgga aaaagagtat ggcattcctc gccgttatct
    45841 cggcaccatc atgtcgccat gggcggcaaa acgcctgcat gaatttggtg gcgatatcac
    45901 taagttccgg gtagtgaagg tctggccgtc aattctgcaa caaattgcta tcgccaaaac
    45961 ggaacccggt gatgagaaca accaggacat ctccgcgctg gttgggaaag tcgatattcg
    46021 taaactcgaa cactacgcgc agaatgaccc ggacgcctac ggctattccg gtgcgctgtg
    46081 ccgcgccaat caggggatca tggaattcgt tgagatgttt aaagcaccga taaaagtgct
    46141 gcatcccttg ttaaccgcca ctcaggaagg taactacaac gggacggaag gtatctccgc
    46201 cctgccgttc aacgggatta ttctcgccca ctcgaacgag tccgaatggg tcactttccg
    46261 taataacaaa aacaacgaag ccttcctcga ccgtgtttac atcgtgaagg tgccgtattg
    46321 cttgcgcatt tccgaagaga tcaaaatcta cgagaaattg cttaatcaca gtgaattgac
    46381 tcacgccccc tgcgcccctg gcacgctcga aacactgtca cgtttttcca ttctttcgcg
    46441 cctgaaagag ccagaaaact ccagcattta ttcaaagatg cgggtttatg atggcgaaag
    46501 tctgaaagac accgatccca aagccaagtc gtatcaggaa tatcgtgact acgccggtgt
    46561 cgatgaaggg atgaacggtc tgtcgacgcg ttttgcgttt aagatcctct cccgcgtgtt
    46621 caacttcgat catgtagaag tggcggcaaa cccagtccat ctgttctacg tcctggaaca
    46681 gcagatcgag cgcgagcagt tcccacaaga gcaggcagaa cgctatctgg agttcctgaa
    46741 aggttatctg atcccgaaat atgccgagtt tatcggcaaa gagatccaga cggcctacct
    46801 tgaatcctat tccgaatatg ggcaaaacat tttcgaccgt tatgttacct acgcggattt
    46861 ctggattcag gatcaggagt atcgcgatcc ggataccggg cagctgtttg accgcgagtc
    46921 tcttaacgcc gagctggaga aaatcgagaa accggcgggg atcagtaatc caaaagattt
    46981 ccgcaacgag attgttaact tcgtactgcg cgccagagcg aataacagcg gacgcaatcc
    47041 gaactggacc agctatgaaa aactgcgcac ggtcatcgag aagaaaatgt tctccaatac
    47101 cgaggagctg ttgccggtta tctcgtttaa cgccaaaacg tcaaccgacg agcagaagaa
    47161 acacgacgac tttgtcgacc gtatgatgga aaaaggctac acccgtaaac aggtgcgttt
    47221 actgtgcgaa tggtatttgc gcgtacgtaa atcgtcttaa caaccctggc ccggtacaca
    47281 tgttaccggg cctacaacga caacgaaccg tgggcctgag aagcggcaac acaggcgtag
    47341 catacagttg gcaaatgtag tacggggggc atatgacctg gtttattgac cggcgtctga
    47401 acggcaaaaa caaaagcatg gtgaatcgcc agcgtttttt acgccgttat aaagcgcaaa
    47461 ttaaacagtc gatctccgag gccattaata agcgttcggt gactgacgtc gacagcggcg
    47521 aatccgtatc cattcccacg gaagatatta gcgaaccgat gtttcatcag gggcgtggcg
    47581 gtctgcgcca ccgcgtgcat ccgggcaatg accatttcgt ccagaacgac cgaattgaac
    47641 gtccccaggg tggcggcgga ggttccggca gtggtcaggg ccaggccagc caggatggtg
    47701 aaggtcagga tgaatttgtc tttcagattt cgaaagatga gtatcttgat ttgctctttg
    47761 aagatttggc cttaccgaat ctgaaacaaa accaacaacg ccagctgacc gaatataaaa
    47821 cgcatcgggc gggttatact gctaacggcg ttccggccaa tatcagcgtt gtgcgttcct
    47881 tgcagaactc actggcgcga cgcacagcca tgacggcagg caagcggcgg gaacttcatg
    47941 cactggaaga gaatttggcc atcatcagca acagtgaacc tgcgcagctg ctggaagagg
    48001 aacgtctgcg caaagaaatt gcagaattac gtgccaaaat tgaacgcgtc ccttttattg
    48061 acaccttcga tttacgttac aagaactacg agaagcggcc cgatccctcc agccaggcag
    48121 tgatgttttg cctgatggac gtttccggtt caatggatca atccactaaa gatatggcta
    48181 agcgttttta tattctgctg tatctgttcc tcagcagaac gtataagaac gtggaagtcg
    48241 tatacatccg ccatcatacc caggcgaaag aagtcgatga acatgagttt ttctactcgc
    48301 aggaaacagg cggcaccatt gtttccagcg ccctgaaact gatggatgag gtagtgaaag
    48361 agcgttataa cccggcacag tggaatattt acgctgcaca agcatcggac ggcgataact
    48421 gggccgatga ctctccgctt tgccatgaaa tcctggcgaa aaaattatta cctgttgttc
    48481 gttattacag ctatatcgaa attacccgtc gtgcacatca gacattgtgg cgagaatatg
    48541 agcatctgca atctactttc gacaactttg cgatgcagca catccgcgac caggatgata
    48601 tttatccggt gttccgtgaa ctgtttcata aacaaaatgc aacagctaaa gactaaaact
    48661 atcagccagg tcattatcgc ctggctgatt tttagcttac tgtaaattat ctcatttatt
    48721 acatacatta gcttacaatc gctttaaata tgacagcata acctttacat aatttagttc
    48781 cagaaaacaa tcattcggaa aaatgattca gtcaacacgt atttccatgg ggttatt
//
LOCUS       DAZKIV010000017        48756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZKIV010000017 DAZKIV010000000
VERSION     DAZKIV010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 48756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.017"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..813
                     /locus_tag="ACHOT6_001017"
     CDS             <1..813
                     /locus_tag="ACHOT6_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HFP9946272.1"
                     /translation="YLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQE
                     QWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSG
                     KIEIYSSRLAEIARTWELEKDEVINPLPVYASTFEGWNSPERRTFPLQLFGFHYKSRT
                     HSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPG
                     VSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            874..3297
                     /gene="ynfF"
                     /locus_tag="ACHOT6_001018"
     CDS             874..3297
                     /gene="ynfF"
                     /locus_tag="ACHOT6_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFP9946273.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKHGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISATMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRRKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSPKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            3308..3925
                     /gene="ynfG"
                     /locus_tag="ACHOT6_001019"
     CDS             3308..3925
                     /gene="ynfG"
                     /locus_tag="ACHOT6_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFP9946274.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            3927..4781
                     /gene="ynfH"
                     /locus_tag="ACHOT6_001020"
     CDS             3927..4781
                     /gene="ynfH"
                     /locus_tag="ACHOT6_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFP9946275.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQASGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            4824..5438
                     /gene="dmsD"
                     /locus_tag="ACHOT6_001021"
     CDS             4824..5438
                     /gene="dmsD"
                     /locus_tag="ACHOT6_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFP9946276.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            5597..6889
                     /gene="clcB"
                     /locus_tag="ACHOT6_001022"
     CDS             5597..6889
                     /gene="clcB"
                     /locus_tag="ACHOT6_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFP9946277.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFHHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6842..7537)
                     /gene="bioD"
                     /locus_tag="ACHOT6_001023"
     CDS             complement(6842..7537)
                     /gene="bioD"
                     /locus_tag="ACHOT6_001023"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9946278.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(7662..8882)
                     /gene="mlc"
                     /locus_tag="ACHOT6_001024"
                     /gene_synonym="dgsA"
     CDS             complement(7662..8882)
                     /gene="mlc"
                     /locus_tag="ACHOT6_001024"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFP9946279.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(9017..9910)
                     /gene="ynfL"
                     /locus_tag="ACHOT6_001025"
     CDS             complement(9017..9910)
                     /gene="ynfL"
                     /locus_tag="ACHOT6_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9946280.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            10017..11270
                     /gene="ynfM"
                     /locus_tag="ACHOT6_001026"
     CDS             10017..11270
                     /gene="ynfM"
                     /locus_tag="ACHOT6_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946281.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(11319..11438)
                     /locus_tag="ACHOT6_001027"
     CDS             complement(11319..11438)
                     /locus_tag="ACHOT6_001027"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFP9946282.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            11694..12002
                     /gene="asr"
                     /locus_tag="ACHOT6_001028"
     CDS             11694..12002
                     /gene="asr"
                     /locus_tag="ACHOT6_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFP9946283.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETATTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKYHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            12095..12178
                     /locus_tag="ACHOT6_001029"
     CDS             12095..12178
                     /locus_tag="ACHOT6_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946284.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            12278..13099
                     /gene="ydgD"
                     /locus_tag="ACHOT6_001030"
     CDS             12278..13099
                     /gene="ydgD"
                     /locus_tag="ACHOT6_001030"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFP9946285.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(13138..13467)
                     /gene="mdtI"
                     /locus_tag="ACHOT6_001031"
     CDS             complement(13138..13467)
                     /gene="mdtI"
                     /locus_tag="ACHOT6_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFP9946286.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(13454..13819)
                     /gene="mdtJ"
                     /locus_tag="ACHOT6_001032"
     CDS             complement(13454..13819)
                     /gene="mdtJ"
                     /locus_tag="ACHOT6_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFP9946287.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            14231..15265
                     /gene="tqsA"
                     /locus_tag="ACHOT6_001033"
     CDS             14231..15265
                     /gene="tqsA"
                     /locus_tag="ACHOT6_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HFP9946288.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(15290..16678)
                     /gene="pntB"
                     /locus_tag="ACHOT6_001034"
     CDS             complement(15290..16678)
                     /gene="pntB"
                     /locus_tag="ACHOT6_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFP9946289.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(16689..18221)
                     /gene="pntA"
                     /locus_tag="ACHOT6_001035"
     CDS             complement(16689..18221)
                     /gene="pntA"
                     /locus_tag="ACHOT6_001035"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFP9946290.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            18745..19689
                     /gene="ydgH"
                     /locus_tag="ACHOT6_001036"
     CDS             18745..19689
                     /gene="ydgH"
                     /locus_tag="ACHOT6_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFP9946291.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            19875..21257
                     /gene="ydgI"
                     /locus_tag="ACHOT6_001037"
     CDS             19875..21257
                     /gene="ydgI"
                     /locus_tag="ACHOT6_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9946292.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            21294..22016
                     /gene="folM"
                     /locus_tag="ACHOT6_001038"
     CDS             21294..22016
                     /gene="folM"
                     /locus_tag="ACHOT6_001038"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFP9946293.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(22013..22348)
                     /gene="ydgC"
                     /locus_tag="ACHOT6_001039"
     CDS             complement(22013..22348)
                     /gene="ydgC"
                     /locus_tag="ACHOT6_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFP9946294.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            22477..23196
                     /gene="rstA"
                     /locus_tag="ACHOT6_001040"
     CDS             22477..23196
                     /gene="rstA"
                     /locus_tag="ACHOT6_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFP9946295.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLVNTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            23200..24501
                     /gene="rstB"
                     /locus_tag="ACHOT6_001041"
     CDS             23200..24501
                     /gene="rstB"
                     /locus_tag="ACHOT6_001041"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HFP9946296.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            24577..25506
                     /gene="tus"
                     /locus_tag="ACHOT6_001042"
     CDS             24577..25506
                     /gene="tus"
                     /locus_tag="ACHOT6_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFP9946297.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGARVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(25503..26906)
                     /gene="fumC"
                     /locus_tag="ACHOT6_001043"
     CDS             complement(25503..26906)
                     /gene="fumC"
                     /locus_tag="ACHOT6_001043"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFP9946298.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEYNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(27049..28695)
                     /gene="fumB"
                     /locus_tag="ACHOT6_001044"
     CDS             complement(27049..28695)
                     /gene="fumB"
                     /locus_tag="ACHOT6_001044"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFP9946299.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            28894..30069
                     /gene="manA"
                     /locus_tag="ACHOT6_001045"
     CDS             28894..30069
                     /gene="manA"
                     /locus_tag="ACHOT6_001045"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFP9946300.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            30170..31678
                     /gene="ydgA"
                     /locus_tag="ACHOT6_001046"
     CDS             30170..31678
                     /gene="ydgA"
                     /locus_tag="ACHOT6_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9946301.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGSQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(31723..32988)
                     /gene="uidC"
                     /locus_tag="ACHOT6_001047"
     CDS             complement(31723..32988)
                     /gene="uidC"
                     /locus_tag="ACHOT6_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFP9946302.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQPEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(33027..34400)
                     /gene="uidB"
                     /locus_tag="ACHOT6_001048"
     CDS             complement(33027..34400)
                     /gene="uidB"
                     /locus_tag="ACHOT6_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFP9946303.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHGSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(34397..36208)
                     /gene="uidA"
                     /locus_tag="ACHOT6_001049"
     CDS             complement(34397..36208)
                     /gene="uidA"
                     /locus_tag="ACHOT6_001049"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFP9946304.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(36597..37187)
                     /gene="uidR"
                     /locus_tag="ACHOT6_001050"
     CDS             complement(36597..37187)
                     /gene="uidR"
                     /locus_tag="ACHOT6_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFP9946305.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(37416..38183)
                     /gene="hdhA"
                     /locus_tag="ACHOT6_001051"
     CDS             complement(37416..38183)
                     /gene="hdhA"
                     /locus_tag="ACHOT6_001051"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFP9946306.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(38295..39323)
                     /gene="malI"
                     /locus_tag="ACHOT6_001052"
     CDS             complement(38295..39323)
                     /gene="malI"
                     /locus_tag="ACHOT6_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFP9946307.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAESITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            39498..41090
                     /gene="malX"
                     /locus_tag="ACHOT6_001053"
     CDS             39498..41090
                     /gene="malX"
                     /locus_tag="ACHOT6_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFP9946308.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            41100..42272
                     /gene="malY"
                     /locus_tag="ACHOT6_001054"
     CDS             41100..42272
                     /gene="malY"
                     /locus_tag="ACHOT6_001054"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFP9946309.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRLLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            42376..43377
                     /gene="add"
                     /locus_tag="ACHOT6_001055"
     CDS             42376..43377
                     /gene="add"
                     /locus_tag="ACHOT6_001055"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFP9946310.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(43412..44452)
                     /gene="ydgJ"
                     /locus_tag="ACHOT6_001056"
     CDS             complement(43412..44452)
                     /gene="ydgJ"
                     /locus_tag="ACHOT6_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9946311.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQAMELIELGI
                     ESAKHRATLCLA"
     gene            44695..44820
                     /gene="blr"
                     /locus_tag="ACHOT6_001057"
     CDS             44695..44820
                     /gene="blr"
                     /locus_tag="ACHOT6_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFP9946312.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            45093..45308
                     /gene="ydgT"
                     /locus_tag="ACHOT6_001058"
     CDS             45093..45308
                     /gene="ydgT"
                     /locus_tag="ACHOT6_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFP9946313.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            45394..45834
                     /gene="ydgK"
                     /locus_tag="ACHOT6_001059"
     CDS             45394..45834
                     /gene="ydgK"
                     /locus_tag="ACHOT6_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFP9946314.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWSVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            45911..46492
                     /gene="rsxA"
                     /locus_tag="ACHOT6_001060"
     CDS             45911..46492
                     /gene="rsxA"
                     /locus_tag="ACHOT6_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFP9946315.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            46492..47070
                     /gene="rsxB"
                     /locus_tag="ACHOT6_001061"
     CDS             46492..47070
                     /gene="rsxB"
                     /locus_tag="ACHOT6_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFP9946316.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            47063..>48756
                     /gene="rsxC"
                     /locus_tag="ACHOT6_001062"
     CDS             47063..>48756
                     /gene="rsxC"
                     /locus_tag="ACHOT6_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFP9946317.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVTRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT        11821 a        12615 c        12032 g        12288 t
ORIGIN      
        1 tatctcattt tcctccagcc tgtcaccagc gaaaaattcg aacgcaaacc gatttactgg
       61 atcctgagtg aagtcgcgaa acgtcttgga cctgacgtct atcaaaagtt cacagaaggt
      121 cgcacgcagg aacaatggtt acaacatctg tacgccaaaa tgcttgccaa agatccggcg
      181 ttaccgtctt acgacgaact gaaaaaaatg ggtatctata agcgtaaaga tcccaatggc
      241 cattttgtcg cctacaaagc atttcgtgac gaccccgagg caaatccact taaaacgcct
      301 tccggtaaga ttgaaattta ttccagcagg ctggcggaaa ttgcccgtac ctgggaactg
      361 gaaaaagatg aagtgataaa cccattgcca gtttatgcct caacctttga aggctggaac
      421 tcccctgaac gtagaacctt cccactgcaa ctgtttggtt tccattacaa atcccgtact
      481 cactcgacct acggcaatat tgatctcctg aaggctgcct gccgtcagga ggtgtggatc
      541 aaccctatag atgcgcagaa acgtgggatt gccaacggcg atatggtgcg ggtgtttaac
      601 catcgtggcg aagttcggct accagccaaa gtaacaccac gtattctccc tggagttagc
      661 gctatgggcc agggagcctg gcacgaggcc aatatgtctg gcgataaaat cgaccatggc
      721 ggctgtgtga atacgctaac cactctgcgc ccttcaccac tggcgaaggg aaacccgcag
      781 cacactaatc tggtcgagat cgaaaaaata taacccacga caaccataaa ttctggcatg
      841 acattttgtt tgaaaagcaa taagtgagta atgatgaaaa tccataccac agaggcgctc
      901 atgaaggctg aaatcagccg tagaagtctg atgaaaacct ccgcacttgg cagtcttgcg
      961 ctagcaagca gtgctttcac tctgccattt tcccaaatgg tccgggcggc agaggctccg
     1021 gtagaagaga aagcggtctg gagttcctgc accgttaact gcgggagccg ctgtctgtta
     1081 cgtttgcatg tgaaagatga caccgtgtac tgggtggagt ctgatacgac aggtgacgac
     1141 gtctacggta accatcaggt tcgagcgtgt ttacgcgggc gctctatccg ccgacggatg
     1201 aatcatcctg ataggttgaa atatcccatg aagcgcgtcg gcaagcacgg tgaaggtaaa
     1261 tttgaacgga taagttggga cgaagccctg gataccatca gtgataatct tcggcggatc
     1321 ctgaaagatt acggcaatga ggctgtacat gtcctgtacg gaacaggcgt agatggcgga
     1381 aacatcacca actcaaacgt cccgtaccgt ctgatgaact cttgcggtgg ttttctcagt
     1441 cgctatggca gctacagtac cgcacagatc agtgccacaa tgagttatat gttcggtgcc
     1501 aatgatggca acagcccgga tgatatcgcc aatacgaaac tggtcgttat gttcggaaat
     1561 aacccggcag aaacgcggat gagcggcggt ggtgtcactt actacgtcga gcaagcccgc
     1621 gaacgttcaa acgcacgcat gatcgtcatc gatccacgtt ataacgacac tgctgccggg
     1681 cgtgaagatg aatggctgcc cattcgccct ggcaccgatg gcgcactggc ctgtgcgatt
     1741 gcctgggtac tgattactga aaacatggtc gatcagccat ttctcgacaa atattgtgtt
     1801 ggttacgatg aaaaaacgct gcccgccaac gcaccacgta acgcgcatta taaagcctat
     1861 attctgggcg aagggcctga cggcatagct aaaacgccgg aatgggcagc aaaaatcacc
     1921 agcatcccgg cagaaaaaat tatccagttg gcacgagaga tcggttcagc aaaacctgct
     1981 tatatttgtc agggttgggg gccacaacga cattccaacg gcgagcaaac gtcccgcgct
     2041 attgccatgc tttccgttct caccggcaac gtcggcataa acggcggcaa ctcaggcgta
     2101 cgcgaaggta gctgggatct gggggtagaa tggttcccga tgcttgagaa tcctgttaaa
     2161 acgcagattt ccgtctttac atggacagat gccatcgacc atggtacgga aatgaccgcg
     2221 acccgtgatg gtgttcgtag aaaagaaaaa ctggatgtcc ccatcaagtt tttatggtgc
     2281 tacgccagta acacattgat caatcaacat ggcgacatca atcacaccca tgaggtgctt
     2341 caggatgaca gcaagtgcga gatgattgtt ggcattgacc acttcatgac ggcctcggct
     2401 aagtattgcg atatcctgtt gcccgacctg atgccaacag agcaagaaga ccttatctct
     2461 catgaatctg cagggaatat gggctatgtg atcctcgccc aacccgcaac ctcaccaaaa
     2521 tttgaacgca aacccatcta ctggatgctg agtgaagtcg ccaaacgctt aggaccagac
     2581 gtttatcaaa cctttactga aggtcgcagt cagcatgaat ggatcaaata tctccatgcg
     2641 aaaacgaagg aacgtaaccc tgagatgccc gactacgagg agatgaaaac gaccgggatc
     2701 tttaagaaaa aatgcccgga agaacactac gtcgctttcc gcgcattccg tgaagatcca
     2761 caggcaaacc cgttgaaaac accttcgggg aaaatcgaaa tttattctga acgactggcg
     2821 aagattgcag atacctggga attgaaaaaa gatgaaatta ttcatcccct tcctgcgtat
     2881 accccaggtt ttgatggctg ggacgatccc ctgcggaaaa cctatccact gcagttaacg
     2941 ggcttccact acaaagcgcg tacccactcc agctacggca atattgatgt gttacagcag
     3001 gcctgcccac aagaggtgtg gatcaacccc attgatgctc aggcacgcgg tatccgtcat
     3061 ggcgataccg tgcgggtatt taacaataat ggagaaatgc tgattgccgc aaaagtgact
     3121 ccgcgtattc tgcctggcgt taccgccatc gggcaaggtg cgtggcttaa agcggatatg
     3181 tttggtgacc gggtcgatca cggcggcagt atcaatattc tgacctctca ccgcccttca
     3241 ccgctggcaa agggaaaccc gtcgcacagc aatcttgtcc agatcgaaaa ggtttaagga
     3301 gtagcccatg accacacaat atggattttt tatcgattcc agccgctgta ctggctgtaa
     3361 aacctgcgaa ctggcgtgca aagattttaa agatcttggc ccggaagtca gtttccgccg
     3421 tatttatgaa tatgctggcg gcgactggca ggaagataat ggcatctggc accagaacgt
     3481 gttcgcttat tatctctcca tctcctgtaa ccattgcgat gaccccgcat gtacaaaagt
     3541 ctgccccagc ggcgcaatgc ataagcgtga agatggcttt gtggtggttg atgaagatgt
     3601 ttgtatcggc tgccgctact gccacatggc ttgtccgtac ggcgcaccac agtacaatgc
     3661 tgaaaaaggg cacatgacga agtgcgatgg ttgttattcg cgcgtcgccg aggggaaaca
     3721 acccatatgc gtcgaatcct gcccgctgcg tgcacttgag ttcggtccaa ttgaagagct
     3781 gcgccagaag cacggcactc tggctgccgt cgcgccactg ccgcgcgcgc atttcacaaa
     3841 acccaatatc gttatcaaac ctaacgccaa cagccgcccg accggcgata ccactggtta
     3901 tctggctaat ccggaggaag tgtaaaatgg gaaatggatg gcatgagtgg ccactggtta
     3961 tctttaccgt attgggtcaa tgcgtagtag gcgcgctgat tgttagcgga attggctggt
     4021 tcgccgccaa aaatgacgca gaccgtcagc gtattgtccg tggcatgttt ttcctctggc
     4081 tgttgatggg catcgggttt attgcctctg tcatgcacct gggatcgcca ctgcgagcgt
     4141 ttaactcgct gaatcgaatc ggagcttctg gtctgagtaa cgagattgct gccggttcca
     4201 tattttttgc cgttggcgga ctatggtggc tggtggcggt catcggtaaa atgccgcaag
     4261 cgtcggggaa actctggtta cttgtcagca tggcgctcgg cgtcattttc gtctggatga
     4321 tgacctgcgt gtatcaaatc gacaccgtgc caacctggca taacggttac actacgctgg
     4381 ctttcttcct gacggtattg ttgagcggtc caatacttgc tgccgcaatt ctgcgggcag
     4441 ctcgcgttac atttaatacc acaccatttg ccatcattag cgttttggcg ctgatcgctt
     4501 gtgccggggt gattgtgctg caaggtttgt ctctggcgtg tattcactca tccgtacaac
     4561 aagccagcgc actggtacca gattacgcct ccttacaggt ctggcgtgtg gtattgttat
     4621 gcgcaggtct aggatgctgg gtttgtccgc tcattcgtcg ccgtgaacct cacgttgccg
     4681 gacttattct ggggttaatc ctcattcttg gcggtgagat gatcgggcgt gtgctctttt
     4741 atggcctgca catgacggta ggaatggcta ttgcaggtta acaaattggt gcggggcaac
     4801 ccgcacattt caggatgtta ggaatgaccc atttttcaca gcaagataat ttttctgtcg
     4861 cggcgcgcgt gttgggcgcg ctgttttatt acgctccaga gagtgcggaa gccgcacctc
     4921 tggttgcggt actcaccagt gacggctggg aaactcagtg gcctctacca gaagcgtcat
     4981 tagcgccgct ggtgacggct tttcagacac agtgtgaaga gactcacgcc caggcctggc
     5041 agcgtttgtt tgtcggcccg tgggcactgc cgtctccgcc gtgggggtcg gtctggctgg
     5101 atcgcgaatc tgtgctgttt ggcgattcaa cattggcact tcgtcagtgg atgcgcgaga
     5161 aaggcattca gtttgaaatg aagcaaaacg aaccggaaga tcattttggt tcactgctgt
     5221 tgatggctgc gtggttggcg gagaatggtc gccagacaga atgcgaagaa ttgctggcat
     5281 ggcacctttt tccgtggtca acacgttttc ttgatgtttt tatcgaaaaa gcagaacacc
     5341 ctttctaccg tgcactgggt gaactggctc gcttaacgct ggcgcaatgg caatcacaac
     5401 tgttgattcc tgtcgcggtt aaaccgctgt ttcgatagga tcaccgtaat attgccggat
     5461 ggtgacgatt acgcgcaatc cggcaataat aggttacagt gtcacgtttt tttatctctt
     5521 aaagcacgca ctgcttttgc ggctggcctc ttttgccgca aaatagtcgt ccgtgtttca
     5581 ttgcccattt ctgctcatgc atcatctaca catctatccg gatctgcgca ctatgttccg
     5641 ccgtctgctt atcgcaacag tcgtcggtat tctcgcggcc tttgccgttg ccgggtttca
     5701 tcatgcgatg ctgctactgg agtggttgtt cctcaataat gactccggca gtctggtcaa
     5761 tgcagcgaca aacctttccc cctggcgacg gctgctaact ccggcgctcg gcggactggc
     5821 ggcgggtttg ttgctgatgg gctggcagaa atttacccaa caacgccctc atgcgccgac
     5881 cgattacatg gaagcgttgc aaaccgatgg acagttcgat tacgcagcaa gcctggttaa
     5941 atcgcttgcc tcgctgctgg tagtaaccag cggcagtgca attggtcgcg aaggtgcgat
     6001 gattctttta gctgcccttg ccgcctcctg ttttgcccaa cgttttacgc cacgccagga
     6061 gtggaaatta tggatcgcct gtggggccgc ggcgggaatg gctgcggcct atcgtgcccc
     6121 gcttgctggc agtttattta tagccgaagt gctgtttggc actatgatgt tggcctctct
     6181 cggcccggtg attatttccg ccgtcgtggc attgctggtt agcaatctga ttaatcatag
     6241 cgacgcgtta ctctacaacg tacaactctc agtgacggtt caggctcgtg actatgcgct
     6301 gattatcagt acaggtgtgc tggcaggtct gtgcggacca ctgttgttaa cgttaatgaa
     6361 cgcctgtcat cgtggatttg tgagtctcaa acttgcgccg ccctggcaac tggcactagg
     6421 cgggttgatc gtgggtctgc tttccctgtt cacacctgca gtgtggggca acggctatag
     6481 caccgtacaa tcctttttaa ccgccccacc actgttaatg atcattgccg ggatcttcct
     6541 ttgtaaactg tgtgccgtgc tggcgagtag cggttccggc gcacccggtg gggtctttac
     6601 accgacgcta tttatcggtc ttgccattgg catgttgtat ggtcgtagcc tgggattatg
     6661 gttccctgat ggcgaagaaa ttacactttt actcggattg accgggatgg cgacactgtt
     6721 ggcggcgacc acgcacgcgc cgattatgtc gacgttgatg atatgtgaaa tgaccgggga
     6781 gtatcagcta ctccccggtt tattgattgc ctgcgtaatt gcgtcggtaa tttcgcggac
     6841 gttacaccgt gactctatct accgccagca cactgcgcag catagctaag cggatgtatt
     6901 gccccagttc acgctgttcg gcgcgcggca gataaggcag ttcaccaatg agcggtgccg
     6961 gaagtttttt acccagcaca tcaatgattt ccgcataatg cgccagtcct gggttgattc
     7021 ggttagccac ccagccaatg agcggcagcc cgtcgttggc aatcgcctga gctgttagca
     7081 gtgcatggtt aatgcaacct tcctgaatac cgacaaccat caacaccggc agttgttcct
     7141 gcactaccca ttcagagagt ggacgcaaat cattcatcag actgcgccag ccgccagtcc
     7201 cttctaccac gacatgatcg actttttcgg tcaggtttgc caggccgttt gaaatgaggg
     7261 tgtaattgat tgggcaactg tgcgccacgc tactttcttc ttcgcttaac gcgataggat
     7321 taactgcttc ataaggcagt tcgatggttg aaacactctg caacaccagg gcatctttat
     7381 tacgcagccc ttcgggtgtc tctttgctcc ccttcgctac gggtttatat cccgcaaccg
     7441 tttttccctg ggaggctaac gcttgtagca atgcgcggga aaccaccgtt ttccctacag
     7501 aagtgtctgt accggtaata aagaaacgct tcagcatcac taactccacc gttatgcttc
     7561 acaaatataa accaggaaaa taattaacct tgaaagtcta agttatgctt tcctggccca
     7621 aattgagata gcgcaaattt tggtagaaca gttaaaaaat gttaaccctg caacagacga
     7681 atcaacaaag aaccgttata catcgcgtct tttaccagcg cagcccctgc catcgtaccc
     7741 tggttagaaa actgagtact ctcaacgctg atgtgctgac tatacgcagg aagggcctgc
     7801 tgacggatgc tgtctgagat gaccgggaag aggatatctg ccgctttact taacggtgag
     7861 ccaatcagta ttttttgtgg gttaaataaa ttcaccatga tggcaagaat gcgccctaca
     7921 tgcgcgccca ccccggtaat gatgtctttt gccagcagat cgccgcgcaa tgccgcctga
     7981 cacaatgagt ccacggttaa cggttgtcca tgtaacatcg agctcatgga ttgattaaga
     8041 cgcagctgtg ccagctcaag aatactgtcc acactggcga tggtttcgag gcagccgtga
     8101 ttcccgcaat aacagcgttt cccatacggg tcgacctgtg tgtggcctat ttccacgaga
     8161 ctactgctgc ctgcgtgtag cagatgacca tcggtaatga cgcccgcccc cacgttgtga
     8221 tcgataacca cctgaatcac atcgcgcgcc ccgcgtgagg caccaaacaa ggcctctgcc
     8281 atcgtccatg cgctaatatc atgctgaata taaaccggta cgccggtatg ctgctccagc
     8341 gcctcgccga gcggcatctc ttttacatcc tcgtagaacg gcatgcgatg tacaatacca
     8401 ttttccgtat caataattcc cggcagggtt atggcaatcg aagttagacg ctcaagtttt
     8461 ttctggtggc ggataaaaaa ctgatcgata tgggaaatga tacgatccag caatggcgag
     8521 tcatctttta acgccagttc ctgcgactct tccaccacca gtttgctgct cagatcgcgc
     8581 agagcaagga aaatctcccc gcgactaatg cgcagagaaa gatagtgcca ggcttcagtt
     8641 tcaaccacca gccccaccgc cggacggcca cggttccccg cttctttgat ttccagctct
     8701 tgcaccaggt gtgcttcgag catctcacgg acaattttag tgatactggc aggagccagc
     8761 tgcgccagac gggaaagatc gatacgcgag actggaccaa gctgatcaat caggcgataa
     8821 accgcgcccg cgttggtctg ctttatttga tcaatgtgcc caggctggtt ttcagcaacc
     8881 accgcatact ccctatattt tcgcgctccg aaataatctg taggctatgg tgaagcactt
     8941 caatacgtgt agtcaaattt ttacttaggc atgtgattaa cagcacattt ttcgggcttt
     9001 ttcgctgaaa tttccctcac ctgagagcat tcagcagatg aatacgaaag tttcgcgcag
     9061 ctggactttg ttcatgatgt ttcggccaga ccaaccacat ttcagaaacc gcatcctctt
     9121 ctgcaatcgg cacccagcgc atttcgttga gctgaacacg tttaaatgac gcaggcaaaa
     9181 ttgaaacacc cagaccggcg gaaaccagac cgatgatggt cattgcctcg cccacctcct
     9241 gagtgatgac gggcgtcaaa tggtaacgtc gcatcagccc gagaatatcg tcatacagcc
     9301 ctgtcccgac gtgcggatca aaaaagacga agggttcttt cgccagttca gccagcgtta
     9361 cattcgggtt atttgccagg ggatgatcgt gcgggatcat cgccataagc ggttcatgga
     9421 cgatgactgc gtgttcaagc gtctccggta acgctgtgtt acgcagcaat cccatatcca
     9481 gcgttccttc aatgagcgga gcgatttgct cacgagtgtt catttcgcgg gtttgtaaat
     9541 ggacatcggg ataatcacgg cgaaacagcg ataacgtatc ggacaccgcc cgaataaaag
     9601 gagcggacga agtaaaacca atgcgcaact cccccgcttc accctgatgc agtctttcag
     9661 cgcgagcggc agcgtcatcc accatagaca ggatttgccg actatctgca agaaactgtt
     9721 ttcctgctgc cgtcagcaat acactgcgat tggttcgtgc cagcagtcgg gcaccaattt
     9781 gttgctccag cgcctgaatc tgctgactta gcggtggttg cgaaatattc aggcgggcag
     9841 cggcgcgccc gaaatgcagc tcttccgcaa cagcaacaaa gtaacgcaga tgacgaagtt
     9901 caatattcat atttaaaaca tcttatttga gattattaat atattagaca gaacaattcg
     9961 attttcctaa cctatgtata agcctgatcc acaggcatat ttagcaagga tttcaagtga
    10021 gccgtactac aactgttgat ggcgctccgg caagcgacac tgacaagcaa agcatttctc
    10081 agccaaatca atttattaaa cgcggtacgc cgcaatttat gcgcgtcacc ctggcgctgt
    10141 tctctgccgg actggcaaca tttgcacttc tctattgtgt gcagcctatc cttccggtgc
    10201 tttcgcagga gtttggctta acgcccgcga acagtagtat ttcactgtcc atttccacgg
    10261 cgatgttggc tattggtttg ctgtttactg gccccctatc cgatgccatt ggtcgcaaac
    10321 cagtgatggt cacggcgcta ctgttggcct ccatttgtac gttactttcg acaatgatga
    10381 ccagctggca cggcattttg attatgcgcg ccttgattgg gctttcgtta agtggcgtgg
    10441 cagctgttgg catgacttat cttagcgagg aaatccaccc cagtttcgtg gccttttcga
    10501 tggggttgta tatcagcggc aactcaattg gcggcatgag cggacgctta attagtggtg
    10561 tcttcacgga ctttttcaac tggcgaattg ctctggcggc aatcggttgt ttcgcgctgg
    10621 cctcggcgtt gatgttctgg aaaatccttc cggaatcacg ccattttcgc ccgacttcgc
    10681 tgcgccctaa gacgttattt atcaactttc gtctgcactg gcgtgaccgg ggattaccgt
    10741 tattgttcgc agaaggcttt ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg
    10801 gctatcggtt gatgctttcc ccctggcatg tcagtcaggc cgtggttggc ttattatcgc
    10861 tggcttattt gaccggtaca tggagctcac ccaaagccgg aaccatgacc acccgctacg
    10921 ggcgcggtcc ggtgatgttg ttttcgacgg gggttatgct gtttggttta ctgatgacct
    10981 tattcagctc gctgtggctg atctttgccg gaatgttact cttctcagca ggattcttcg
    11041 cagcccactc agttgccagc agctggattg gcccccgcgc aaaacgcgct aaaggccagg
    11101 cctcctcgct gtatctgttc agttactatc tggggtcgag tattgccggg acgctgggtg
    11161 gtgttttctg gcataactat ggctggaacg gcgtcggcgc atttattgct ctgatgctgg
    11221 tcattgctct gctggtcggg acgcgtttgc atcgtcgtct gcacgcctga aaaataagcc
    11281 cggactgcgg taactacccg tccggactta ttgcttgctc aaaccaacgt taatagccat
    11341 cctaaaatag acgaagcgcc agccaattcc cgcagcgcgt ctagcgtcat caggattata
    11401 agtacccaaa taaacggatt cattttgctg tgtgtcattt attactgatg cgcagttatt
    11461 ctaccgcttt gtaagtagta aaatagttaa cccgatcaag actactatta ttggtagcta
    11521 aatttccctt aagtcacaat acgttattat caatgctgta atttattcag cgtttgtaca
    11581 tatcgttaca cgctgaaacc aaccactcac ggaagtctgc cattcccagg gatatagtta
    11641 tttcaacggc cccgcagtgg ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa
    11701 aagtattagc tctggttgtt gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag
    11761 agactgcgac cacacctgct ccgactgcga cgaccaccaa agcagcgccg gcgaaaacga
    11821 cacatcataa aaaacagcat aaagcagcac ctgcccagaa agcgcaggcg gctaaaaagt
    11881 atcataaaaa tacgaaagct gaacagaaag cccctgaaca aaaagcgcag gcagcgaaga
    11941 aacacgccgg gaaacacagc catcagcaac cggcaaaacc tgctgcacaa cccgcagcgt
    12001 aagtttacaa cagtaatgct ggcgcgcccc ctcgcgcctg aaaattacgg tgctaagcgg
    12061 gtaacgttta gcaccgcctt tagcggaggg tagtatggtg ggccgttatc gctttgagtt
    12121 cattcttatc atccttattt tatgcgcact gattgccgcc cgtttttatc tttcctgact
    12181 gtagttatct gattttactc ccactttcat cccgtcccgt ctatagtatt tatgagggtt
    12241 tgcttttaat aatcataatt acccaccaga gtgtgatatg cgtacaacca ttgctgtagt
    12301 gttgggtgca attagtttga cgtctgcttt tgtgtttgca gataaaccag acgttgccaa
    12361 atcggcaaac gatgaggtca gcaccctgtt ttttggtcat gatgatcgtg tgccagtgaa
    12421 tgacacgacc caatcaccgt gggatgcggt tgggcaactg gaaacggcca gcggcaattt
    12481 atgtacggcg acgctgattg cacccaatct ggcattaacg gcaggacact gtttattgac
    12541 gccgcctaag ggtaaagcgg ataaggcggt agcgctgcgt tttgtgtcaa ataaaggtct
    12601 ttggcgctat gacatccacg acatagaagg ccgcgttgat ccgacactgg gaaagcggtt
    12661 aaaagcagat ggggatggtt ggattgttcc tcccgcagct gcaccatggg acttcggctt
    12721 gattgtgctg cgtaatcccc cttctggcat tacaccgttg ccgttatttg agggagataa
    12781 agccgcgctt actgccgcat taaaatcggc tggtcgtaaa gtgactcagg cgggatatcc
    12841 tgaagatcat ctcgatacgt tatacagtca tcaaaactgt gaagtgactg gctgggcgca
    12901 aacgtcggtg atgtcacatc agtgcgatac cttgccgggt gacagcggtt cgcctctgat
    12961 gttgcatacc gatgacggct ggcaattaat tggggtgcaa agttcggctc ctgccgcgaa
    13021 agatcgctgg cgcgccgata accgggccat ttctgttacc ggttttcgcg acaagctgga
    13081 tcaactgtcg caaaaataat gttcaaacgc tgcccgactg tgcgggcagc gtgttcatca
    13141 ggcaagtttc accatgatca ttccagccaa cagcaagacc aggccaatcc agcctttacg
    13201 atttaaccgt tgaccaaaca agatccaacc tgcggctaac gtggcggcaa taccaaaccc
    13261 gccccacaat gcataagcga cagacaagtc gatcccttta acggcctgag aaagcgcact
    13321 aaaggcagcc agcaccgccg ccagggagag caagccaaat attttgcgac gaaagccgtc
    13381 agaaaatttc aaaaagacgt tagcaacgat ttccagcacg attgccaatg ccagccaggc
    13441 ggcgtgaacc cattcaaact gcgccatggt tcacctccag ttcaggttta cgcgctttac
    13501 gggtacctga ttttatcaac acaatcccgg cgaccagggt ggttaacccg gcaattttca
    13561 tcagcgataa actttcgtcg aataacaaaa cgctaaacaa ggtaataaat aaaataccga
    13621 taccttccca cagcgcataa gctacgccta aggcgatttt tttaacggcg aaagagagaa
    13681 atatatacga cagagaaatc atcaccagca ttaaaataaa gccgccattt ccctcactga
    13741 cgctcgccca tttcatggac agcgtaccgg taatttctgt agcaatagcc agacctaata
    13801 aaatccaata aatatacatt gtccttctcc tgcaagagaa ttattttaat tttcgcttaa
    13861 ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata gggcaagctt aggcagaaga
    13921 aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga gcaagatagt ggatgaggta
    13981 cgaagatggg taaatgtaga aaataacgtc ctgaacaaat tgtccataat attacaatta
    14041 tccgcagtgt tgcttctcgt catcgcggat gataattgtc ctcggtagtt gaacacgcct
    14101 gatttgtatc atagcttaag aattaactca aaatattttc acttctttac ctgagcggtt
    14161 tgattttcgt tatgatgacg gagcgaaaaa gacattatta ttagcaaagg aagaaaaaac
    14221 ggggacaagc atggcaaagc cgatcatcac gctcaatggc ctaaaaatcg tcattatgtt
    14281 gggaatgctg gtcattattc tctgcggtat ccgttttgcc gccgagatca tcgtgccgtt
    14341 tattctcgca ttatttattg ctgttattct taacccgctg gtgcaacaca tggtccgctg
    14401 gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc atcatcgtga tggcgatggt
    14461 gttgctatta gcttatctgg gttccgcgct caacgagttg acgcggacgt taccgcaata
    14521 tcgcaactct attatgacgc cgctgcaagc tcttgagccg ttgttgcaac gcgtagggat
    14581 tgacgtctca gttgaccagc tggcgcatta cattgatccg aacgcggcga tgacgttgct
    14641 caccaactta ttgacgcagt tatctaatgc catgtcatca atatttttat tgctgctgac
    14701 ggtgctgttt atgctgctcg aagtgccaca attgcccgga aaatttcagc aaatgatggc
    14761 gcgtccggtt gaagggatgg cggcgattca acgtgcaatt gacagtgttt ctcattatct
    14821 ggtgctgaaa acagccatca gcatcatcac cggcctggtc gcctgggcga tgctcgccgc
    14881 actcgatgtt cgcttcgctt ttgtctgggg attgctggcc tttgcgctta attacatccc
    14941 taatattggt tcagttctcg cggcaatccc ccctatcgct caggtactgg tgtttaatgg
    15001 cttctacgaa gcgttgctgg tgctggcggg atatctgctg attaatctgg tcttcggcaa
    15061 tattctggag ccgcgcatca tgggacgtgg gctggggctt tccacattgg tggtattttt
    15121 gtcgttgatt ttttggggat ggttgttagg accggtgggt atgctgcttt ccgtgccgtt
    15181 aacaattatt gtcaaaattg cgcttgaaca aacagcggga ggtcaaagca tcgccgttct
    15241 gttaagcgat ctcaataaag agtgacggcc tccgcagagg ccgtcagggt tacagagctt
    15301 tcaggattgc atccacgctg gctttggcgt caccaaacag catgtgggtg ttttccttga
    15361 agaacagcgg gttttgcaca ccagcatagc cagtgttcat cgaacgttta aagacaatca
    15421 cgttctgcgc tttccacact tccagcacag gcataccagc aatcggactc ttcggatcat
    15481 cctgcgccgc cgggttaacc gtatcgttag caccaatcac cagtacggta tcggtatcag
    15541 caaagtcatc gttgatctcg tccatttcca gcacgatgtc atacggtact tttgcttcag
    15601 ccagcaatac gttcatatgt ccaggcaaac gccccgcaac cgggtggata ccgaaacgca
    15661 cgttgatacc acgagcgcgc agtttctcgg taatttcagc gacaggatat tgcgcctgcg
    15721 cgactgccat gccgtacccc ggagtaatga tcactgaatg ggagtttttc agcagttccg
    15781 ctgtctcttc tgcggtgatt tcgcggtgct cacccacttc ctgatcatcg ccagtagaag
    15841 agccgtcggt gccgaaacca cccgcaataa cgctgataaa ggaacggttc atcgccttac
    15901 acataatgta agaaaggata gcccccgaag aaccgaccag cgcaccggtc acaatcagca
    15961 ggtcgttgct gagcataaag cccgcagccg cagccgccca gccggagtac gagttcagca
    16021 tcgacaccac cactggcata tctgcaccac cgatggaggc gactaaatgc cagccgaata
    16081 ccagcgcaat tgcggtcatt atcagcaatg ccagcacttg caggccgacg ctgtccgtgc
    16141 gaacaaatac aatcagcagc aggaaggaaa cgaccagagc cgccaggttc attttgtgac
    16201 ggtttggcag catcaatggt ttagacgaaa tcttgccaca cagtttgccg aacgccacca
    16261 ccgaacccgt gaacgttacc gccccgatga agataccgag gaacacttcc gtcaggtgaa
    16321 tattgaccag aatcggtgcc attcccgcgt catgatgcag atagctgtta aagccaacca
    16381 gcactgccgc cagacccacg aagctatgca ggatcgccac cagttctggc atttcggtca
    16441 tttcaacttt cttcgccaga cggataccaa ttgccccacc aatgaccatc gccagcaaga
    16501 tccagccaac attacccgta tccggtccaa aaatggttgc gattaacgca atcgccatcc
    16561 cggcgatacc gaagttgtta ccctggcgag acgtttcatg tttcgaaaga ccggccagac
    16621 tgaagataaa caggatcgcg gcaacaatgt atgcagctgt aactaatcct ccagacatat
    16681 gttacccctt aatttttgcg gaacattttc agcatgcgct gagtcacggt gaagccaccg
    16741 aaaatattaa tgctggctat aagcaccgcg ataaaactaa ggaagctaac ccagccgccc
    16801 tggccaatct gcaacagtgc tccgacaaca ataatccctg aaatcgcgtt ggtgaccgac
    16861 atcaacggtg tatgcagcgc gtgcgataca ttccacacca cgtaataacc gacaacgcag
    16921 gccagcgcga aaacggtgaa gtgcccaaga aactctttcg gcgcaacgct tgccagccaa
    16981 ccgaaaagga tgattgccag cgccatcaac gcgtatttac gccacggtga gcaggcacat
    17041 ttttcctcag ttttcacttc cggtgccgct ttttgtgccg cctgcggctg agctgatacc
    17101 tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga tcacggtcac gccgcgaatc
    17161 accacatcat caaaatcaac agtgatattg ccgtctttct ctttgcacaa cagtttcagc
    17221 agattaacga ggttagtacc gtaaagctgc gaggattgcg tcggcagacg gcccggaaga
    17281 tcggtataac caatcacttt gacaccattt tccgtagtga agatttcacc cggcacggtg
    17341 tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa tcacactgcc cgccttcatg
    17401 gagtcaacca tttcacgggt aattagcttc ggcgctggtt tgcctggaat aagcgcggtg
    17461 gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga gttccatttc cgctttaatg
    17521 aacgcgtccg acatcacttt ggcatagcca tcgccgctgc ccgcttcctc tttaaaatcc
    17581 agctcgagga attccgcgcc catactttga acttgttctt tcacttccgg acgggtgtcg
    17641 aatgcacgca caatcgcgcc gagactgttt gctgcgccaa tggcggccag acctgcaaca
    17701 cccgcaccaa tcaccatcac ttttgccggt ggcactttcc cggccgcagt aatttgcccg
    17761 gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg cgcgataacc ggcgatgttc
    17821 gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga tacgcggcac agaatccatc
    17881 gccatcacgg tcacgttacg ttccgcaagt ttttgcatta attccggatt ctgcgcaggc
    17941 cagataaaac tcaccagcgt tgtcccagga ttcagtaacg caatttcatc atctaacggc
    18001 gcattgacct tcagaatgat ctctgactgc cagacgctat tcccttctac aatttcagcg
    18061 cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt gacccgcgcc gctctctacc
    18121 gcgacggtaa aacccagttt cagcagctgt tccactgttt ttggcgttgc tgcaacacgg
    18181 gtttcattgg ttaaccgttc tcttggtatg ccaattcgca tgatattccc ttccatcggt
    18241 tttattgatg atggtttgcc tgtgtcagga gccacacaag ccgctcatgt acgagctaaa
    18301 tgttactccg ttaaaataaa ttagtaacaa acgccctata acgtactgaa aattatgcct
    18361 gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg caaaatatta acgattcagc
    18421 cgtgatagtg ggataaacac cttagaacgc cggataaaga ctgataattg tcttcgacgg
    18481 tcgggtaaaa cgagacatcg ccccggcacg attcactact taacattaaa ttaacttata
    18541 caattcagtt gcttcagtag taatgatgct gatacggctg ttttttaagc atagacggtc
    18601 atttgagcag gattaaaatt ggcttaagga atgtgatatg aaaaatgacg cagacagtta
    18661 caccgtttaa atgcaataat cagccacgtt tctcgttaat aacaatacca gtacctggtt
    18721 tgcgcaaggc gaaggattat ttttatgaag cttaagaaca ccctcctggc gtcggcactg
    18781 ctttctgcta cggcattctc cgttaacgca gcaacagaac tgacaccgga gcaagcggca
    18841 gcggttaaac cttttgaccg tgtagtggtt accggtcgtt ttaatgctat tggcgaagcg
    18901 gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg cctcttttta tgttgtcgac
    18961 acttctgatt ttggtaacag cggtaactgg cgtgtggtcg ctgacctcta taaagccgat
    19021 gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg gtgttgtcga actgccgaaa
    19081 gatcaggctg ttctgattga accgtttgac acggtcaccg tccagggctt ctatcgtagc
    19141 cagccagaag tcaatgatgc catcaccaaa gcggcaaaag cgaaaggtgc ctactctttc
    19201 tacatcgttc gtcaaatcga tgccaaccag ggcggcaacc agcgtattac tgcattcatc
    19261 tataaaaaag atgctaagaa acgtatcgtc cagagcccgg atgtgatccc ggcagattcc
    19321 gaagcaggac gtgcagctct ggctgccggt ggcgaagccg cgaagaaagt tgagatccca
    19381 ggtgttgcga ctaccgcatc accaagttct gaagtcggtc gtttctttga aacccagtca
    19441 tcaaaaggcg gacgttacac tgtcacgctt cctgacggca ctaaagtcga agaactgaac
    19501 aaagcgacag cagcgatgat ggtcccgttc gacagcatca aattctccgg caactacggc
    19561 aacatgaccg aagtctccta tcaggttgcg aaacgtgccg cgaagaaagg tgctaagtat
    19621 taccacatca cccgccagtg gcaggaacgt ggtaataacc tgaccgtcag cgcagatctg
    19681 tataaataac tattcagtta ctcctataag gcggcttgat gccgcctttc gcatttttag
    19741 tcacaaattt ccccgcgacc attgcatgca gacacatctc ttcgtaaaat cccgcgcttc
    19801 agtggcaaac tccattttta tgcgttttgc cctaatattt attcttctta tcacgtttta
    19861 atcactggat atcgatggaa aagaaactgg gactgagcgc actcaccgcg ctggtattaa
    19921 gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa tatggcggca gttgccagcc
    19981 cggcagcact gctcatcggc tggggtatta ctggcgctgg cattttattg ctggcctttg
    20041 ccatgctgat cctcacgcgc attcgtcccg aacttgacgg cggtatcttt acctatgccc
    20101 gcgaagggtt tggcgagcta atcggctttt gttccgcatg gggatactgg ctgtgcgcag
    20161 tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc gttaagcttt tttacggaca
    20221 cgccggaatt gcgcctgttc ggcgatggta atacctggca atcgatagtc ggtgcatcgg
    20281 ctttattgtg gattgttcac tttttgatcc tgcgcggggt gcaaaccgct gccagcatta
    20341 acctggtggc gacactggca aaattgttgc cgctgggtct gtttgttgtg ctggcgatga
    20401 tgatgttcaa actggatacc ttcaagctcg acttcaccgg acttgcactt ggcgtacccg
    20461 tgtgggaaca ggtgaaaaac accatgctga tcaccctgtg ggttttcatt ggtgtggaag
    20521 gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga tgttggcaaa gcgacactgc
    20581 tggcggttct ctccgctctg ggcgtttact tgctggtaac gctgctttcg ctgggcgtgg
    20641 tggcacgtcc tgaactggct gaaattcgta acccgtcaat ggccggtctg atggtggaaa
    20701 tgatgggacc atggggcgaa atcatcatcg ctgccggttt gatcgtttcc gtttgcggcg
    20761 cgtacttaag ctggaccatc atggcggcag aagttccgtt cctggctgcc actcataaag
    20821 cattcccacg catttttgcg cgccagaacg cacaagctgc gccatccgcc tcgctgtggt
    20881 tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg gctgaccggt tccgattaca
    20941 acacgttgct gaccatcgcc tcagaaatga ttctggtgcc ctatttcctg gtcggcgcgt
    21001 tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt aggtgtcggt gcctgcattt
    21061 atggcttatg gttattgtat gcctctggtc caatgcactt gctgttatcc gttgttctgt
    21121 atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac gcatacacat gataatgtac
    21181 tgaaccgtca ggaaatggtg ctgattggca tgctgttaat tgcctcagtt ccggcgacct
    21241 ggatgctggt gggataacgt tcatcccatc gttggatagc aaaggagata acgatgggta
    21301 aagcccagcc cttgccaata ttaattactg gcggaggtcg tcgcatcggc ctcgccctcg
    21361 cctggcattt cattaatcaa aagcaaccgg tgattgtcag ctatcggacg cactatccag
    21421 ccattgatgg actgattaag gcaggtgcgc agtgtattca ggctgatttt tcgaccaacg
    21481 acggtgtgat ggcgtttgcc gatgaagtac taaaaagcac ccatggtctg cgtgctattt
    21541 tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc gccactggcc gacgtactgg
    21601 cttgcatgat gcagatccac gttaataccc catacctgct caaccatgcg ctggaaagat
    21661 tactgcgtgg gcacggacac gccgccagcg atatcattca ctttaccgat tatgtggtgg
    21721 agcgcggtag cgacaaacat gttgcgtatg ctgcaagcaa agcggcactg gataatatga
    21781 cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt gaattctatt gcgccatcgc
    21841 tgatcctgtt taatgaacat gatgatgccg aatatcgaca acaggcgctg aataaatcac
    21901 tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct ggtcgattac ttacttacca
    21961 gctgctttgt caccggacgc agtttcccac ttgatggcgg tcgtcatctg cgttaatgca
    22021 gctttatcca gcatataatc aacacccatg cgcttatccc ccaacacgca acagaaccaa
    22081 caaaagcggc gggcaagcgc atcattccgg tgaaatacca cagcgacacc aggtaaacaa
    22141 aatagggaat aatcgaccac atactaaaaa tgatggttgc gcgtaaggct tcaatgccgc
    22201 gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt cgggaaaagt ggaatcagcc
    22261 cggcgatata ataatttttc gtttttgcta aaacaccaat caacagcact accagcgcac
    22321 cgagcgcggc tttgattacc agccccatct ttttacctta acacttccat aacaagtcat
    22381 cagtagaata cctgatgaaa acttgtttag aaacgattga tagtaagtaa aaacagcgcg
    22441 gtgtattgtg acgtttttat atctaccgtg aatgttatga acactatcgt atttgtggaa
    22501 gatgatgcgg aagtcggttc actgattgcc gcgtacctgg caaaacatga tatgcaggtt
    22561 accgtagaac cgcgcggcga ccaggccgaa gaaaccattt tgcgagaaaa tccggatttg
    22621 gtgttactcg acatcatgct accaggcaag gacggcatga ccatttgtcg tgatttacgc
    22681 gcaaagtggt ctggaccgat tgttcttcta acctctctcg atagcgatat gaaccacatc
    22741 ctggcactgg aaatgggtgc ctgcgactat attctcaaaa cgacgccccc tgctgttttg
    22801 ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag ccacactgac caaaggtctt
    22861 caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg gcacgttgac catcgatccc
    22921 atcaaccgcg tagtcaccct ggttaacact gaaatctcgc tctcgacagc tgatttcgaa
    22981 ttattgtggg aattagctac ccatgccggg caaatcatgg accgcgatgc attgctgaaa
    23041 aatttacgcg gcgtcagtta tgacggactg gatcgtagcg tggacgtggc tatttcgcgg
    23101 ttaagaaaaa aactgctcga taacgccgca gaaccttatc gcattaaaac tgtgcgtaac
    23161 aaaggctatc tttttgcgcc tcatgcatgg gaataagcga tgaaaaaact gtttatccag
    23221 ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc tgctggttgg gttggtgtac
    23281 aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg atttgatgaa cagttcgctg
    23341 tatctgatgc gcagcgaatt gcgtgagatc cccccacacg actggggtaa gacgctgaaa
    23401 gagatggatt taaatctctc tttcgatctg cgtgtcgagc cattgagtaa ataccatctt
    23461 gatgatattt ccatgcaccg actgcgtggc ggcgaaattg tcgccctgga cgatcagtac
    23521 acgtttttgc agcgtatccc gcgcagccac tacgtgctgg cagttggtcc tgttccttat
    23581 ctttattatc tccatcagat gcgattgctg gatatcgccc tgatcgcttt tattgctatt
    23641 tccctcgcct ttccggtgtt tatctggatg cgtccgcact ggcaggatat gttaaaactg
    23701 gaagcagcgg cgcaacgatt tggcgatggg catctcaatg agcgtatcca ctttgatgag
    23761 ggttcgagct ttgaacgact tggtatcgca tttaaccaga tggcggacaa tatcaacgcc
    23821 ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc acgaactgcg aacaccgtta
    23881 gtgcgcctgc gttatcgact ggagatgagc gataacctga gcgccgccga atcccaggcg
    23941 ttgaatcgtg atatcagtca acttgaagct ttaattgaag agctgctgac ttatgcccga
    24001 ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag acctgccgtt gtggctgtca
    24061 acgcatctgg cagatattca ggcagtaacg cccgataaaa cggtacggat aaaaacgctc
    24121 gtgcaaggcc attatgcggc gttggatatg cgcttaatgg agcgcgtgct ggataatttg
    24181 ctcaataacg ccctgcggta ctgccattca acggttgaaa ccagcctgct actgtcgggg
    24241 aatagagcga cattaattgt tgaggatgat ggcccaggga ttgccccaga aaaccgcgaa
    24301 catatctttg aaccttttgt tcgcctcgat cccagccggg atcgctcaac cggcggctgc
    24361 gggctggggc tggcaattgt ccactctata gcactggcaa tgggcggtac ggttaattgt
    24421 gacaccagcg aactgggtgg tgctcgcttc tcgtttagct ggccgttatg gcataacatc
    24481 ccgcaattta cctctgcctg acactacgcg cacgatggtc aagtcaccac gactgtgcta
    24541 taaaataagt atgttgtaac taaagtggtt aatattatgg cgcgttacga tctcgtagac
    24601 cgactcaaca ctacctttcg ccagatggaa caagagctgg ctgcatttgc cgctcatctt
    24661 gagcaacaca agctattggt tgcccgcgtg ttctctttgc cggaggtaaa aaaagaggat
    24721 gagcataatc cgcttaatcg tattgaggta aaacaacatc tcggcaacga cgcgcagtcg
    24781 ctggcgttgc gtcatttccg ccatttattt attcaacaac agtccgaaaa tcgcagcagc
    24841 aaggccgctg tccgtctgcc tggcgtgttg tgttaccagg tcgataacct ttcgcaagca
    24901 gcgttggtca gtcatattca gcacatcaat aaactcaaga ccacgttcga gcatatcgtc
    24961 acggttgaat cagaactccc caccgcggca cgttttgaat gggtgcatcg tcatttgccg
    25021 gggctgatca cccttaatgc ttaccgcacg ctcaccgttc tgcacgaccc cgccacttta
    25081 cgctttggtt gggctaataa acatatcatt aagaatttgc atcgtgatga agtcctggca
    25141 cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac cgtggacgcg cgaggagtgg
    25201 caaagaaaac tggagcgaga gtatcaggat atcgctgccc tgccacagaa cgcgaagtta
    25261 aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg tctggtacaa aggagatcaa
    25321 aaacaagtcc aacacgcctg ccctacaccg ctgattgcac tgattaatcg ggataatggc
    25381 gcacgcgtgc cggacgttgg tgagttgtta aattacgatg ccgacaatgt gcagcaccgt
    25441 tataaaccac aggcgcagcc gcttcgtttg atcattccac ggctgcacct gtatgttgcg
    25501 gattaacgcc cggctttcat actgccgacc atctgttctg gccgtaccca gctgtcaaac
    25561 tcggcttcgc taagataccc cagcgcaagg gccgcagctt ttaaggtcag cccttcttta
    25621 tgcgcttttt tggcgatctc cgcggctttg tcataaccaa tgtgggtgtt aagcgcagtc
    25681 accagcatca gcgattcatt gagtaattga ttgattcgct cacgattcgg ttcaataccc
    25741 actgcgcagt gtttgttaaa actttccatg ccatctgcca gcaagcgcac cgattgcagg
    25801 aaattgtgga tcaccattgg acggaagacg ttcagttcaa agttaccgga agcgcccccc
    25861 atgttgatcg ccacgtcgtt ccccatcacc tgacagcaga gcatggttaa tgcctcacac
    25921 tgtgttggat tcactttccc cggcatgatt gagctgcccg gctcattttc cgggattgag
    25981 atttcaccaa ttccgcagcg cgggccagag gccagccagc ggacatcatt ggcgattttc
    26041 atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct gaaccagagc atcacaggtc
    26101 gccagcgctt caaatttgtt cggcgcggta acaaacggtg cacaggtaat gactgccagt
    26161 tcatctgcta cgcgacgcgc atactccgga tgggtattta gtccagtacc caccgctgta
    26221 ccgccaagag ccagttccgc tacgtgaggc aggctgtatt cgatatgttt gagattatac
    26281 tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg ttagcggcgt ggcgtcctgc
    26341 aagtgggttc gaccgatttt gacgatatcg gcaaatgcac gcgatttttc actcagcgtc
    26401 tgtgtcaggg ttttaagctg cggaatgagt tgcttgcgca gcgccagcag cgccgcaacg
    26461 tgcatcgccg tcggaaagac atcgttggaa ctttggcttt tgttcacgtc gtcgttaggg
    26521 tgaactttac gttccatccc gcgcacgccg ccgagtaatt cactggcccg gttagccagc
    26581 acttcgttca tgttcatgtt actttgcgtg ccggagccgg tctgccagat agccagcggg
    26641 aattcgtcgt catgctgtcc tgccagtact tcatccgccg cctgccgaat ggcgctcgct
    26701 ttctcttcag acaacaagcc taaatcttca ttaacttttg ccgctgcgcg cttggttagc
    26761 gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg aaatgcggaa atgctccagc
    26821 gagcgttgag tttgtgcgcc ccacagctta tctgccggga catcaatcgc ccccatcgaa
    26881 tctttttcgc tgcgtactgt attcatggcc tgctcctcac ctgtttaatt ttttctttct
    26941 gttttgcttt cgttaagcaa cttttagctc acttattatt tactatttga taacaaatgt
    27001 ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc ggctctgttt atttcacaca
    27061 gcgagtgcac tgtgtgagtt gtatctgctg gaagaagtca tttcctttat catccacaag
    27121 gataaacgcc gggaaatctt ccacttcaat tttccagatg gcttccattc ccagttccgg
    27181 atattcaaca cattccaggc tcttaatact tccctgcgcc aatacagcgg ccggaccacc
    27241 gatactgcca aggtagaagc cgccgtgttt tttacaggca tccgtcacct gctggctgcg
    27301 gttgcctttc gccagcatga tcatacttcc gccctgcgct tgcagttgat cgacataaga
    27361 atccatccgt cctgcggtcg ttgggccaag agaaccggag gcataacctt ccggcgtttt
    27421 ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac tgcggcagcc cttcaccgtt
    27481 atccatccgc tctttcagtt tggcgtgagc aatatcacga ccgacgataa tcgtgccgtt
    27541 aagcgataag cgtgtagaaa cgggatactg cgacaactgt gcgaggatct ctttcatcgg
    27601 acggttaagg tcaacacgca ccgcttcgcc ttctcccgct ttacgcagct cttcagggat
    27661 atatttacct ggattatgtt ccagtttttc gatccagatc ccctgacggt tgatcttcgc
    27721 tttgatatta cggtcagcag agcaggaaac acccatacca accggacagg atgcgccgtg
    27781 acgtggcagg cgaatcacgc gtatgtcgtg agcgaagtat ttaccaccaa actgcgcacc
    27841 cagaccgaga ttttgcgctt cgatcagcaa ttctttttcc agttccacat cgcggaatgc
    27901 ctgaccgtgc tcattccctt ccgttggcag ttcgtcatag tatttagcgg aagccagttt
    27961 cactgtttta aggttagttt ctgcagaagt tccaccaatt acgaacgcaa tatgatacgg
    28021 aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc aggtaatttt tcagtttccc
    28081 cggcgttagt aacgctttgg tttcctgata gagatacgtc ttgtttgccg aaccaccacc
    28141 tttggcgata cagaggaatt tgtactcgtc gccatcaacg gcataaagat cgatctgcgc
    28201 tggcagattg gtgccggtat tcacctcttt atacatatcc agcggcgcgt tttgcgagta
    28261 gcgcaaatta tcttcgatat aagtgttata gacaccgcgc gccagcgccg cttcatcaca
    28321 gccaccggtc catacacgct gccctttttt accaacaata atcgcagtgc cggtatcctg
    28381 acaggttggc agaacgcctt tcgccgcgat gtcggagtta cgcaggaatt gcagcgccac
    28441 atatttatca ttttcgctgg cctccgggtc acgcagaatg tcggccacct gttgttggtg
    28501 agccggacgc agcataaacg acgcatcatg aaacgcctga cgcgccaaca gagttaacgc
    28561 ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca gatacgctaa cgtgttcgct
    28621 ggttagcagg taatactcag tatcatcttt tttgagtgga aaaggagcct gataatgaaa
    28681 gggtttgttt gacattgttc tctcacttac tgcctggttt ggttatgctc tgggcgggtg
    28741 ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac acttttttaa caaaaactga
    28801 gactagtacg actttttgcg gctccaggtt acttcccgta ggattcttgc tttaatagtg
    28861 ggattaattt ccacattaaa acagggattg atcatgcaaa aactcattaa ctcagtgcaa
    28921 aactatgcct ggggcagcaa aacggcgttg actgaacttt atggtatgga aaatccgtcc
    28981 agccagccga tggccgagct gtggatgggc gcacatccga aaagcagttc acgagtgcag
    29041 aatgccgccg gagatatcgt ttcactgcgt gatgtgattg agagtgataa atcgactctg
    29101 ctcggagagg ccgttgccaa acgctttggc gaactgcctt tcctgttcaa agtattatgc
    29161 gcagcacagc cgctctccat tcaggttcat ccaaacaaac gcaattctga aatcggtttt
    29221 gccaaagaaa atgccgcagg tatcccgatg gatgccgccg agcgtaacta taaagatcct
    29281 aaccacaagc cggagctggt ttttgcgctg acgcctttcc ttgcgatgaa cgcgtttcgt
    29341 gagttttccg agattgtctc cctactccag ccagtcgcag gtgcacatcc ggcaattgct
    29401 cactttttac aacagcctaa tgccgaacgt ttaagcgaac tgttcgccag cctgttgaat
    29461 atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa aatcggcgct cgacagtcag
    29521 cagggtgaac cgtggcaaac gattcgttta atttctgaat tttacccgga agacagcggc
    29581 ctgttctccc cgctgttact gaatgtggtg aaattgaatc ctggcgaagc gatgttcctg
    29641 ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc tggaagtgat ggcgaactcc
    29701 gataacgtgc tgcgtgcggg tctgacgcct aagtacattg atattccgga actggttgcc
    29761 aatgtgaaat tcgaagccaa accggctaac cagttgttga cccagccggt gaaacaaggt
    29821 gcagaactgg acttcccgat tccagtggat gattttgcct tctcgctgca tgaccttagt
    29881 gataaagaaa ccaccattag ccagcagagt gccgccattt tgttctgcgt cgaaggcgat
    29941 gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac cgggtgaatc agcgtttatt
    30001 gccgccaacg aatcaccggt gactgtcaaa ggccacggcc gtttagcgcg tgtttacaac
    30061 aagctgtaag agcttactga aaaaattaac atctcttgct aagcttatta aaggcttata
    30121 acaccttcag gcggccagtc cgcctgattt cattttatgg ataatcatta tgaataaatc
    30181 gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc tggacaggcg gcgcatggta
    30241 tacaggcaag aagattgaaa cccatctcga agacatggtc gcgcaggcga acgcgcaact
    30301 caaactgaca gctcctgaat ccaacctgga agtgagttat caaaactatc atcgcggcgt
    30361 attcagcagc cagttgcaac tgttggtgaa acccattgcc gggaaagaaa atccgtggat
    30421 taaaagcggt cagagcgtca tcttcaacga atcggttgat catggtccct tcccgcttgc
    30481 ccagcttaaa aaactgaacc tgatcccgtc gatggcatca attcaaacca cgctggttaa
    30541 taacgaagta agcaaaccac tgtttgatat ggcaaaaggt gaaacgcctt ttgagattaa
    30601 ctcgcgcatt ggttacagcg gtgattccag ttccgatatt tcgctcaagc cactgaatta
    30661 cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag ttccagttaa atgctgacag
    30721 agacggcaaa gccatctccc tttccgggga ggcgcaaagt ggtcggatag acgcagttaa
    30781 cgaatacaac cagaaagtgc agttgacctt taataatctg aaaaccgacg gttccagcac
    30841 gctggcaagt tttggtgagc gcgtaggaag ccaaaaactg tcactggaaa aaatgaccat
    30901 ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg gagatcagcg gtaaatcgga
    30961 tctggtcaat gacggtaaaa cgatcaatag ccaactggat tactcgctaa acagcctgaa
    31021 ggtacagaat caggatctgg gcagcggcaa gctgacttta aaagtcggcc agattgatgg
    31081 tgaagcctgg catcagttta gccagcaata taacgcgcaa actcaggcgc tgctggcaca
    31141 gccagaaatt gccaacaatc ccgaacttta tcaggagaaa gtgacggaag ccttctttag
    31201 cgccctgccg ctgatgttga aaggcgatcc ggtgattact atcgcgccgc taagctggaa
    31261 aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc ctgaaagatc cggcaacgac
    31321 taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt tcggttaaat ctctggatgc
    31381 gaaactgacc attccggtgg atatggcaac tgagtttatg actcaggtag cgaagctgga
    31441 aggttatcag gaagatcaag cgaaaaaact ggcgaaacag caagttgaag gtgcatcagc
    31501 aatggggcag atgttccgtc tgaccacctt gcaggacaat accatcacca ccagcctgca
    31561 atatactaac ggtcagataa cgttaaacgg gcagaaaatg ccactggaag atttcgttgg
    31621 tatgtttgca atgccggcat taaatgttcc ggtcgtaccc gctattccgc agcagtaatt
    31681 cacagacaaa acgccggaga tttttctctc cggcgttatt ttttacttca gcataaagtc
    31741 atactgaata ctcattttca catcccagta attcaggcga tcgcccagcg tactgccatc
    31801 gcgcttttcc cgccctttat cggcagcaag aaagaaattc atcccttgta acggcccttc
    31861 gctgacccga tagttcatac cacctaagat acccgtgcct tcaatccgca catctcgttt
    31921 actggattca tagccgcgag tcacaatcgg tgcaaatgtc agcgtaaatt gcggcgttaa
    31981 acgatagttg acgcccaatt gccaggactg cattccttca cggttacgat caagacttaa
    32041 tgaaaaggga tagccaatgt cggtatccac cacggcacca ttcattgcat ggcgcaaata
    32101 accaccactg ccaaatacgc ttccccacgg cgcattccag gtcaattgtc cactaaccaa
    32161 cgccgtttca ttgggctggc tggtattacg acttaaacct tcgggctgcg cgtaaaagcc
    32221 gagcaactcc cctttcaacg tataattcgg gtcgagcggt atgcttttct cgagaatact
    32281 ttgtatctga ttggaaacat ctttctgtac cgatgccccc agagaatagc gactggtatc
    32341 atcatgccaa ctggcggcta aaaaagaacg tgcgcgatgt ttatcgtatt ttcccgtctg
    32401 gctgttatac accccttcat cacgaatacc ggttgttgaa tcagtccata aaaatactcg
    32461 tgaacgccac attccccaca gatcaatatt tggtgttaaa gcccaataac ctaatgctcc
    32521 ttcagaaact gtcggtaatg tacgttgcga actgccagcc attaacggaa tacgataagg
    32581 taagctacca taactataat ccgtcccgaa gcgacctaat ttaagcaggc tattttcact
    32641 gggttttatt tttactgccc ccagggcaaa gccaaaactt ttatcaccat caagatacca
    32701 ccgggtactc atatcagcac gagcacctaa tttataaaca taatatgccc cgccttcaac
    32761 gccaataata ttggaataat aaccactatt gaaatcgagc aatcctccct gtacccatgc
    32821 gtaaatatct cttcccgcgc cagcactggg cttatccgcc ctgcgcaatt cgtttttcag
    32881 tctgatgcgt agtcccgctt catcgtcagc cagttgcgcc gcataagcag aggtaaggcc
    32941 agaggcagcc gtcaggcaaa taacggccat ggccactatt tttctcatag ttgcaccttt
    33001 gatgttctga ttatttttat tgaatattaa ttagtgatat cgctgattaa ttgctgctgc
    33061 acttttttac gattatcaat ttcaaccacg atttctttga attttttatc cgtgagcgga
    33121 taaaaccaga taataacgaa tgccagtagc ataaatccgc aaggtactaa ggcaattgat
    33181 gtgcggatgc ccataataac ttccggcgtt tgcacctgat tggcgatata tccgcttaat
    33241 cccaaaataa aggcaggaat tgaacctccg attgcctgac cgcatttacg ggtaaatgag
    33301 aatagtgaat aggtgagccc ttcaattcgc acgccggtca ggtattcacc gtattctacg
    33361 gtatcagctt ccagtgccca catcacagtc atagtaacgc cctgaccaat tgaagcgatg
    33421 gccaacgcaa ccaacgccac cggcagtgac cagacggaaa cccagaagaa tagcagataa
    33481 ccgcaggttc ccagcaaagc gccaatcagg aaggtattct ttttaccgat cctcgcgacc
    33541 atccccggca ccagcggtgc cgatgccaca gtaccaacca ggttttgcac cagtaccagc
    33601 acagtgaaca gcccggtatc atttaacaca tagcgcacgt agaacaacga cgaggcgctg
    33661 accgcgaagg tcgaaatcag cacacacagc gcaccgatgc acaacataaa cagcgggcga
    33721 ttccgtttca gggtttgcag actgatcttc aatgacggct gcgccacgat acgtaccaca
    33781 ttctcacgcg tcgatttgaa gcagatgaag taaagcacca ttccggcaat cgccagcaca
    33841 atcgtccaga aatggtatac cgacaccatc tcttccgggc tggagttctt aatgctcggt
    33901 cctatcagaa atgccaggca gacaaaggtt aatgaagcgg caatcccgcg agccgcgccc
    33961 agacgggcgc gggattgtgg ttgttgggtc atcgcggtag caagtgaacc ataaggaata
    34021 ttcaccaggc tgtagcaaag cccgaggccc atgtaggtca aatatgcata caccactttg
    34081 ctaccatggc tccagtcggt cggcacccag aataccagca cgctgaagat cattaacggc
    34141 gcagtaccga agagtaaaaa cgggcggaat tttccccagc gggtattcac actgtccacc
    34201 actcgtccgg caaagacgtc ggcgaaggca tcgaataccc gcaccagtaa cagcatggtg
    34261 cccgccgcag cggcaccgac gccagcgacg tcggtgtagt aactcaatag gaagagcgcc
    34321 cccattgcga aggcgaagtt attggcgacg tcaccgaggc tgtagccgac gatggtgcgc
    34381 caggagagtt gttgattcat tgtttgcctc cctgctgcgg tttttcaccg aagttcatgc
    34441 cagtccagcg tttttgcagc agaaaagccg ccgacttcgg tttgcggtcg cgagtgaaga
    34501 tccctttctt gttaccgcca acgcgcaata tgccttgcga ggtcgcaaaa tcggcgaaat
    34561 tccatacctg ttcaccgacg acggcgctga cgcgatcaaa gacgcggtga tacatatcca
    34621 gccatgcaca ctgatactct tcactccaca tgtcggtgta cattgagtgc agcccggcta
    34681 acgtatccac gccgtattcg gtgatgataa tcggctgatg cagtttctcc tgccaggcca
    34741 gaagttcttt ttccagtacc ttctctgccg tttccaaatc gccgctttgg acataccatc
    34801 cgtaataacg gttcaggcac agcacatcaa agagatcgct gatggtatcg gtgtgagcgt
    34861 cgcagaacat tacattgacg caggtgatcg gacgcgtcgg gtcgagttta cgcgttgctt
    34921 ccgccagtgg cgcgaaatat tcccgtgcac cttgcggacg ggtatccggt tcgttggcaa
    34981 tactccacat caccacgctt gggtggtttt tgtcacgcgc tatcagctct ttaatcgcct
    35041 gtaagtgcgc ttgctgagtt tccccgttga ctgcctcttc gctgtacagt tctttcggct
    35101 tgttgcccgc ttcgaaacca atgcctaaag agaggttaaa gccgacagca gcagtttcat
    35161 caatcaccac gatgccatgt tcatctgccc agtcgagcat ctcttcagcg taagggtaat
    35221 gcgaggtacg gtaggagttg gccccaatcc agtccattaa tgcgtggtcg tgcaccatca
    35281 gcacgttatc gaatcctttg ccacgcaagt ccgcatcttc atgacgacca aagccagtaa
    35341 agtagaacgg tttgtggtta atcaggaact gttcgccctt cactgccact gaccggatgc
    35401 cgacgcgaag cgggtagata tcacactctg tctggctttt ggctgtgacg cacagttcat
    35461 agagataacc ttcacccggt tgccagaggt gcggattcac cacttgcaaa gtcccgctag
    35521 tgccttgtcc agttgcaacc acctgttgat ccgcatcacg cagttcaacg ctgacatcac
    35581 cattggccac cacctgccag tcaacagacg cgtggttaca gtcttgcgcg acatgcgtca
    35641 ccacggtgat atcgtccacc caggtgttcg gcgtggtgta gagcattacg ctgcgatgga
    35701 tcccggcata gttaaagaaa tcatggaagt aagactgctt tttcttgccg ttttcgtcgg
    35761 taatcaccat tcccggcggg atagtctgcc agttcagttc gttgttcaca caaacggtga
    35821 tacgtacact tttcccggca ataacatacg gcgtgacatc ggcttcaaat ggcgtatagc
    35881 cgccctgatg ctccatcact tcctgattat tgacccacac tttgccgtaa tgagtgaccg
    35941 catcgaaacg cagcacgata cgctggcctg cccaaccttt cggtataaag acttcgcgct
    36001 gataccagac gttgcccgca taattacgaa tatctgcatc ggcgaactga tcgttaaaac
    36061 tgcctggcac agcaattgcc cggctttctt gtaacgcgct ttcccaccaa cgctgatcaa
    36121 ttccacagtt ttcgcgatcc agactgaatg cccacaggcc gtcgagtttt ttgatttcac
    36181 gggttggggt ttctacagga cgtaacataa gggactcctc attaagataa aaatactggt
    36241 caacctttaa tctgattagt taaccaattc tgtgttgcac acagttatct gtgagaaacc
    36301 catggaagag aaatggaaat aaaaagaact gacaccgcga tcaaaaaacc aaaaactgca
    36361 atacccacag cgtgatattg atcgcattaa tctttaaaac ggcgttgaca gaagccataa
    36421 aataagcaac cgaacgttcg ttcgagtata ttgggaacga taaactctca tgacatatgg
    36481 ctacggtgaa tatttttggg ggggaattct ccgatcccgg ctagattgta ttttgtacag
    36541 ggaaattaaa gtatgaaacg cgggcacagt tatcaccagc ccgcgaaatg agaggatcag
    36601 gatgcggtta agataccgcc aatcatcgcg cgtaatccct gagcaaacga cgcttcacgc
    36661 gccagatctt ccgcttcgat atcggccagt acaccgtagg tcagatccag cagtaaacgt
    36721 gaagtcattg acgtgttgat gtctgggctt atctcgcctt tttgctgggc atcacgcatc
    36781 cgctgggcaa caaattcagt gatcgtcata tgcttatttt taagcatggc ggcgacctgt
    36841 gggttacgca tcccttccgc cataatttcg accaccagcg cccgttgccc aaaggcttca
    36901 tgggtgagag agacaataga ctcgaccata tagtcaacaa aatgaatccc ttcaatcggt
    36961 tcccggaaac gggccagcgc cctctcctgg tcctgtaaga taatcgcctg aatcaaggct
    37021 tctttggaga tgaaatggtg atagagcgtc ccgggactaa tggcgcagga tttacagatg
    37081 gctttcatcg aggcactgtg aaatccattt tctgaaaaaa tctctctggc agcattgagg
    37141 atccgggtcc gtgtcggttg tgcttcagtc tgcatgttat ccatcattaa accattatag
    37201 ttagcgtgaa taactagttt accgcgttgt caggtgagcg cctatcattg ctgttgaata
    37261 gtgcgaaggc acactctatt cgtatgcttt tcatccagct attggttaac caatagccgt
    37321 gatttggctg ccagttgttt agcagagacg ttgatgaatt gcacacgcta tcccggtaaa
    37381 caacacatac ggctgtttac cggttcgtta gtgtattaat tgagctcctg taccccacca
    37441 ccggagacgg tgagaatttg tccgcttacc cagctggctg caggcgagca aaggaacagc
    37501 gccgcgttag caatatcttg cggttggccc agacgtctga tcggcgtgtg ctgtaacatt
    37561 ttttgttcaa tttctggtgt aataacggat ttcagggcat cggttaatat tgcccccggc
    37621 gcaatgccat tcacccgaat atttttttca cccaggtcaa atgccatatt tctgaccaga
    37681 tgactggccg cagctttaga tgatgcatag gaagtcatgt ttatattttt attttctgcc
    37741 gccatagaag tgatggtcag aataacgcca ccgccatttt tttccatttc tggcgcaaca
    37801 agttgtgaca gatggaaaaa agaaaacaca ttcagttcat aagcacggcg aaaatctgcc
    37861 attggcatat caaacggttt aggaccaccg ccaccggcgt tgttaaccag gatatcaact
    37921 ttacccagct tactgacggc aaagtctgcc agtgcagaga gttcctgttc ggaagtaata
    37981 tcacaacggc aggcaaatgc ctgaccaccc agttgttgaa tttcgtctac aacatggtta
    38041 gctgcgtcgg cgttaatatc actgaccacc acagatgcgc cagctgtcgc gaatgtaatg
    38101 gcgatttctt taccaatacc tgcacccgca cctgtgatga tggcgcattt tccgtcgagt
    38161 ctcaggttgt cagaattaaa cacataaacc tcctgtgaac ttcagtaaaa ataaaaggca
    38221 ccgcctgtgt gccacatttt ttatcattaa ccttgaggaa aggacactac caggtatagc
    38281 agataaagag aggattattt cgccgcaatg agccgggcgg gaataataag attgcgtgaa
    38341 tgcgtctctt catgggtgat tttttgcatc atgcgatccg caagtgtgat accaagttcc
    38401 cgcgctggcg tgctggccca ggtaacaggt atatcatcaa gtgtggttgg tgtcgcatcg
    38461 gtaaatgccg ccagcgaaac ctgttgctca aagtaacgat cgactccgct ttccccgctt
    38521 tgccgccctg ctttgagcaa accaaaccat gcccccatcg caatagtttc gttatagcaa
    38581 accacggcac tgatggtcgg gttatgacgt aaaagcgccg tgatagattc cgcggcttgc
    38641 ttctggctgg aagtgcactc caacacccaa tcgctatgaa acggcaggcc aaattttagt
    38701 agagttgcac aatagccccc cacccgttct gcacgggtta atgaggaact ttgccctccc
    38761 agccaggcga tccgctgatg cccattgcga atgagatgct ccgtcaacaa ctgtgcagcc
    38821 tgcatgttgt ccgggcgaac cgtatcaaca tcatcaagat aactggcacg ggaagcgaaa
    38881 atcaccggga tagctttttc ttctgccatc cgtcgcaggt catcgctact tcctgcagct
    38941 ccggcaatta ccacaccatc gacaccctga ttcagtaaca gtgaaaaccg ctgtgccagc
    39001 tgctcaccgt ctttaccgcc gtgaagcaaa aaaaccatcc gtccctgcgc ttccagagct
    39061 tccgtcaatc cggccgtcaa ttcggcgtaa aacggcgcag ataaatcacg gacgatcaaa
    39121 ccaatgacgc cgctttgccc gccgcgcagc gccgacgcct ggcgattgcg cacaaatccc
    39181 agctcttcaa tggcggcgtt aacgcgttct cctgtggcgg tagagattcg ccctttgcca
    39241 ctaagcacca gcgaaacggt acttaccgac acgcccgcag ccagcgcaac atcatgaatg
    39301 gttatttttt tggcggtagc catgcagatg acctactccc tgattatgtg acagataaaa
    39361 cgttttacct tttatttatc ttatacccgc tattatcgtt gcgtaatgtg atttatgcct
    39421 cactaaaatt tgataaaacg ttttatcttc tcgcgcaatt tactgaatcc agattgttct
    39481 ctacgaggag tcgttttatg acggcgaaaa cagcaccgaa agtcacgctg tgggagttct
    39541 tccagcagtt aggcaaaacc ttcatgttac ccgtggcatt attgtcgttc tgcggcatta
    39601 tgctcggcat tggtagttct cttagcagcc atgatgtcat aaccctgatc ccggtcctgg
    39661 gcaaccccgt gttgcaggct atctttacct ggatgagtaa gattggctcg tttgctttta
    39721 gtttcctgcc tgtcatgttc tgtatcgcca tcccgctggg cctggcacgc gaaaataaag
    39781 gcgtagcggc attcgctggc ttcgtcggtt atgcggtaat gaacctcgcg gtaaacttct
    39841 ggttgaccaa taaaggcatt ctgccaacca cggatgccgc ggttctgaaa gccaataaca
    39901 tccagagcat tcttgggatc cagtcgatcg ataccgggat cctcggtgcg gtgatcgccg
    39961 gtattatcgt ctggatgctg catgagcgtt tccataatat ccgcctgccg gatgcgctgg
    40021 cattcttcgg cggtacgcgc ttcgtaccaa ttatctcctc gctggtgatg ggccttgtcg
    40081 gcctggtgat tccattagtc tggccgattt tcgccatggg tattagcggc ttaggccata
    40141 tgatcaacag cgcgggtgat ttcggaccga tgctgtttgg taccggtgaa cgtctgctgt
    40201 tgccgtttgg tctgcatcac attctggtgg cattaattcg ctttaccgac gcaggcggca
    40261 cgcaggaagt ctgcggtcaa accgtcagcg gcgcactgac catcttccag gcgcaattga
    40321 gttgcccgac cactcacggt ttttctgaaa gcgccacgcg tttcctttcg caaggcaaaa
    40381 tgcctgcgtt tctcggcggt ctgccaggtg cagcgttagc tatgtatcac tgcgcgcgcc
    40441 cggaaaatcg ccataaaatt aaagggctgc tgatttctgg cctgatcgcc tgtgtcgttg
    40501 gcggcacgac cgaaccgctg gaattcctgt tcctgttcgt agcgccagtt ctgtatgtca
    40561 tccacgcgct gttaaccggc ctcggcttca ccgtcatgtc tgtgctcggc gtcaccatcg
    40621 gtaataccga cggcaatatc atcgacttcg tggtgttcgg tattttgcat ggtctgtcaa
    40681 ccaagtggta catggtgcca gtggtggcgg caatctggtt cgtcgtttac tacgtcatct
    40741 tccgtttcgc tatcacccgc ttcaatctga aaaccccggg gcgcgatagc gaagtggcca
    40801 gttcaatcga aaaagccgtt gccggtgcgc cgggtaaatc aggttacaac gttccggcaa
    40861 tcctcgaagc cttaggcggt gccgacaata ttgtcagcct cgataactgc attacccgtc
    40921 tgcgtttgtc tgtgaaagat atgtcgcttg ttaatgtgca ggcactgaag gacaatcggg
    40981 caattggcgt agtacaactt aatcaacata acctgcaggt tgttatcggg ccacaagttc
    41041 agtcagtaaa agatgaaatg gccggtctga tgcatactgt ccaggcataa ggataagata
    41101 tgttcgattt ttcaaaggtc gtggatcgtc atggcacatg gtgtacacag tgggattatg
    41161 tcgctgaccg tttcggcact gctgacctgt taccgttcac gatttcagac atggattttg
    41221 ccactgcccc ctgcattatc gaggcgctga atcagcgcct gatgcacggc gtatttggct
    41281 acagccgctg gaaaaacgat gagtttctcg cggctattgc ccactggttt tccacccagc
    41341 attacaccgc aattgatacg cagacggtgg tgtatggccc ttctgtcatc tatatggttt
    41401 cagaactgat tcgtcagtgg tctgaaacag gtgaaggcgt ggtgatccac acgcccgcct
    41461 atgacgcatt ttacaaggcc attgaaggta accagcgcac agtaatgccc gttgctttag
    41521 agaagcaggc tgacgactgg ttttgcgata tgggcaagct ggaagccgtg ttggcgaaac
    41581 cagaatgtaa aattatgctc ctgtgtagcc cacagaatcc taccgggaaa gtgtggacgt
    41641 gcgatgagct ggagatcatg gctgacctat gcgagcgtca tggtgtgcgg gttatttccg
    41701 atgaaatcca tatggatatg gtctggggcg agcagccgca tattccctgg agtaatgtgg
    41761 cgcggggaga ctgggcgttg ctgacgtcgg gctcgaaaag tttcaatatt cccgcgctga
    41821 ccggtgctta cgggattata gaaaatagca gcagccgtga tgcctattta tcggcactga
    41881 aaggacgtga tgggctttct tccccttcgg tactggcgtt aactgcccat atcgccgcct
    41941 atcagcaagg tgcaccgtgg ctggatgcct tacgcgtcta tctgaaagat aacctgacgt
    42001 atatctcaga taaaatgaac gccgcgtttc ctgaactcaa ctggcagatc ccgcaatcca
    42061 cttatctggc ctggcttgat ctacgtctgt tgaatattga cgacaacgcg ttgcaaaaag
    42121 cgcttatcga gcaagaaaaa gtcgcgatca tgccggggta tacctatggt gaagaaggtc
    42181 gtggttttgt ccgtctcaat gccggctgcc cacgttcgaa actggaaaaa ggtgtggctg
    42241 gattaataaa cgccatccgc gctgttcgct aaccccaatt gcgcaacgta aaaaatcgtt
    42301 gcgcaatcgt ggatttttac cctgctttgt ttttataatg gtgcgcactt ttatattcag
    42361 aaaaagagtg cgaccatgat tgataccacc ctgccattaa ctgatatcca tcgccacctt
    42421 gatggcaaca ttcgtcccca gaccattctt gaacttggcc gccagtataa tatctcgctt
    42481 cctgcacaat ccctggaaac actgattccc cacgttcagg tcattgccaa cgaacccgat
    42541 ctggtgagct ttctgaccaa acttgactgg ggcgttaaag ttctcgcctc tcttgatgcc
    42601 tgtcgccgcg tggcatttga aaacattgaa gatgcagccc gtcacggcct gcactatgtc
    42661 gagctgcgtt tttcaccagg ctacatggca atggcacatc agctgcctgt agcgggtgtt
    42721 gtcgaagcgg tgatcgatgg cgtacgtgaa ggttgccgca cctttggtgt gcaggcgaag
    42781 cttatcggca ttatgagccg gaccttcggc gaagccgcct gtcagcaaga gctggaggcc
    42841 tttttagccc accgtgacca gattaccgca cttgatttag ccggtgatga acttggtttc
    42901 ccgggaagtc tgttcctttc tcacttcaac cgcgcgcgtg atgcgggctg gcatattacc
    42961 gtccatgcag gcgaagctgc cgggccggaa agcatctggc aggcgattcg tgaactgggt
    43021 gcggagcgta ttggacatgg cgtaaaagcc attgaagatc gggcgctgat ggattttctc
    43081 gccgagcaac aaattggtat tgaatcctgt ctgacctcca atattcagac cagcaccgta
    43141 gcagagctgg ctgcacatcc gctgaaaacg ttccttgagc atggcattcg tgccagcatt
    43201 aacactgacg atcccggcgt acagggagtg gatatcattc acgaatatac cgttgccgcg
    43261 ccagctgctg ggttatcccg cgagcaaatc cgccaggcac agattaatgg tctggaaatg
    43321 gctttcctca gcgctgagga aaaacgcgca ctgcgagaaa aagtcgccgc gaagtaacaa
    43381 aatggatggt gcaaattgca ccatccattt ttcatgcaag gcacaaagtc gcgcgatgtt
    43441 tggcggattc gatgcccagc tcaatcaact ccattgcctg gattgcctgg cttgccggaa
    43501 ccggattttc accatcgcca tttaacgcat cacgaatagc cgcatagtaa gccggataat
    43561 tcccaggcac cgtcaacagc gtttcttcga cacgttcctc accttccacg cgggtaagta
    43621 cgccatcacg catatcgtag ccccagtctt cctgcggtag acgctcgcca tttttcagac
    43681 gttcttcctg tggatcgagg ccatatttca cataactgcc tcgggatcca tgcacgatat
    43741 accgtgctga ctcagcggct gccagcatgg taccgtgtaa aatgactcgc cgctgtggat
    43801 aggacaagat ggcgtggaaa taatcggtcg actgcgctcc gggccgtaac tgtgccaaat
    43861 ctaccgtcat gctgaccggt aaaccaaata gcgtaatggc ctgatcaaga agatgcggtg
    43921 ctaaatcgta ccagataccg ctgcctgggc cgccctgttc acgccaacga tcgcgcacct
    43981 gcggacggaa gcggtcaaaa tgagactcaa agtaagcaac ttcacccagc acgccttccg
    44041 cgagcaaacc ttttagcgtc aagaaatcgc tatcccaacg acggttatgg aatacagaca
    44101 gcacacgccc caggcttttt gccagcgcat ccagctctcg cgcttgtgac agtgtcacgg
    44161 taaagggttt atcaacgacc acatgtttac ccgcctcaag cgccgctttg gctaacggga
    44221 aatgggtatc gttgggtgta ggaatgacaa tcaggtctat gttgggatcg ttaaacagat
    44281 gcttcggctc agagacaacc gtaaccgttg gccagtcggc ttttactttt gtttcatcac
    44341 tgctggagat taccgccagt tccagcccgg gcgtgcccgc aatcaggggc gcatggaagg
    44401 ttttgctcgc ataaccatac ccaatcaacc caacacggat gttgtcgctc atgtcattgc
    44461 ctctcattga agtatgatgg ctatttgaca ctatccttta cccacgctca acagtttaat
    44521 aacctgccag caataaggga tgttgtttaa cttaagtcaa aaaaatagcg aattttccaa
    44581 cgacaaaagc taaatatcgc aaaaacctca gtaaaaatct tgctggagct attattgcta
    44641 agtaacattt acaccctgaa gttaatggat caatcaagag agatgtgggc tgtaatgaat
    44701 cgtcttattg aattaacagg ttggatcgtt cttgtcgttt cagtcattct tcttggcgtg
    44761 gcgagtcaca ttgacaacta tcagccacct gaacagagtg cttcggtaca acacaagtaa
    44821 gctctgcgct tgtagagcgc catgctgccc gtccgggtgc atgttttcac ttgtcggata
    44881 ttaaaccagg aatttattat cttgttcgat gttgttggtg attgtcaggg atagtaaagc
    44941 gcgttactct tttcagagca gtttttcatc cctgaatatt tgccatcagc gcattcttat
    45001 tattgtaata acacaagcgt tcacttttgg tgaaatttta tcatcactaa taatacctac
    45061 ccctttggta ttaattattt tatggacctt ttatgactgt tcaggactac ttattaaaat
    45121 ttcgcaaaat cagttcactc gaaagtctgg aaaaactcta cgaccatctt aattacaccc
    45181 tgacggacga tcaggaactg atcaatatgt atcgtgctgc cgatcaccgt cgcgcagagc
    45241 tggtttctgg cgggcgtttg tttgacctcg gccaggtacc gaagtccgtc tggcactatg
    45301 tccaataaag aaagtagcga tcggttataa aagccttata ataacgcccc taaaattaga
    45361 tttgcctgtt tgcgtcgcta ttctggagaa cctatgacca ccacaacgcc acaacgtatt
    45421 ggaggctggt tgcttggccc gttggcctgg cttttagtcg ctctgttgag tacgacgctg
    45481 gcgttgttgc tgtataccgc tgcgttatct tctccgcaaa catttcaaac gctcggcgga
    45541 caagcactga cgacacaaat cttatggagc gtttctttta ttaccgctat cgccatgtgg
    45601 tattacacac tgtggttgac catcgctttc tttaaacgtc ggcgttgtgt tcctaaacac
    45661 tatattatct ggctgcttat ttccgtattg ctggcagtaa aagcattcgc tttttcaccc
    45721 gtggaagatg gcatagccgt tcgccagttg ctgtttactt tgttggcaac cgcattgatc
    45781 gtcccctatt tcaaacgctc gtcgcgggta aaagcgacgt ttgtgaatcc gtaataacct
    45841 tacagttaac ctgttgtcgc ctgctctgga ttaacggata ataggcggct tttttatttc
    45901 aggccgaaaa atgactgact acctgttact gtttgtcgga actgtactgg tcaataactt
    45961 tgtactggtc aagtttctcg gtctctgtcc gtttatgggg gtttccaaaa aactggaaac
    46021 cgcgatgggc atggggctgg caacaacgtt tgtgatgacg ctggcgtcta tttgcgcctg
    46081 gcttatcgat acgtggattt tgatcccact taatctgatt tacctgcgca ccctggcatt
    46141 tattctggtg attgctgtgg tcgtgcagtt caccgagatg gtggtgcgca aaaccagccc
    46201 ggtgctttac cgtctgctgg ggattttttt gccgcttatc accaccaact gtgcagtgct
    46261 cggcgtggcg ttgctgaata tcaatctcgg gcacaatttc ttgcagtcgg cgctgtacgg
    46321 tttttccgcc gctgtcggtt tctcgctggt gatggtgctc ttcgccgcca tccgcgaacg
    46381 ccttgctgtg gctgatgtcc cggcaccttt tcgcggtaat gccattgcgt taattaccgc
    46441 aggtcttatg tctctggcct ttatgggctt tagtggtttg gtgaagttgt aatgaatgct
    46501 atctggattg ccgttgccgc cgtgagcctg ctgggcctgg cgtttggcgc cattctgggt
    46561 tatgcctccc gccgttttgc ggtggaagac gatccggtcg ttgagaaaat tgacgaaatc
    46621 ttaccgcaga gccagtgtgg tcagtgcggt tatcccggct gtcgccccta cgcggaagcc
    46681 atcagctgta acggtgaaaa aatcaaccgt tgcgccccag gtggcgaagc tgtgatgcta
    46741 aaaattgccg agttgcttaa tgtcgagccg cagccgctgg atggcgaagc gcaagagcta
    46801 acgcctgcgc ggatggtggc ggttattgat gaaaataact gtattggctg cactaaatgt
    46861 attcaggcgt gtccggtaga cgccatcgtt ggcgctaccc gtgccatgca tacggtaatg
    46921 agtgatctct gtacgggctg caatttatgt gttgatccgt gcccgacgca ctgcatctcg
    46981 ttgcaaccgg tcgcagaaac acctgactcc tggaaatggg atctgaacac cattcccgtg
    47041 cgtatcattc ccgtggaaca ccatgcttaa gttattctct gcattcagaa aaaataaaat
    47101 ctgggatttc aacggcggca tccatccacc ggagatgaaa acccagtcca acggtacacc
    47161 cctgcgccag gtaccactgg cgcagcgttt tgttattcca ctgaaacagc atattggcgc
    47221 tgaaggtgag ttgtgcgtta gcgtcggcga taaagtattg cgcggccagc cgcttacccg
    47281 tggtcgcggc aaaatgctgc ctgttcacgc gcccacctcg ggtaccgtta cggctattgc
    47341 gccccactct acggctcatc cttcagcttt agctgaatta agcgtgatta ttgatgccga
    47401 tggtgaagac tgctggatcc cgcgcgacgg ctgggccgat tatcgcactc gcagtcgcga
    47461 agagttaatc gagcgcatac atcagtttgg tgttgccggg ctgggcggtg caggattccc
    47521 gacaggcgtt aaattgcagg gtggcggaga taagattgaa acgttgatta tcaacgcggc
    47581 tgagtgcgag ccgtacatta ccgccgatga ccgtttgatg caggattgcg cggctcaggt
    47641 cgtagagggt attcgcattc ttgcgcatat tctgcagcca cgcgaaattc ttatcggcat
    47701 tgaagataac aaaccgcagg cgatttccat gctgcgcgcg gtgctggcgg actctaacga
    47761 tatttctctg cgggtgattc caaccaaata tccttctggc ggtgctaaac aattaaccta
    47821 cattctgacc gggaagcagg ttccacatgg cgggcgttca tccgatatcg gcgtattaat
    47881 gcaaaacgtc ggcactgctt atgcagtgac acgtgccgtt attgatggcg agccgattac
    47941 cgagcgtgtt gtaaccctga ctggcgaagc aatcgctcgc ccgggcaacg tctgggcacg
    48001 gctggggacg ccagtgcgtc atttattgaa tgatgccgga ttctgcccct ctgccgatca
    48061 aatggtgatt atgggtggcc cgctaatggg ctttaccttg ccatggctgg atgtcccggt
    48121 cgtaaagatt accaactgtc tgttggctcc ctctgccaat gaacttggcg aaccacagga
    48181 agaacaaagc tgcatccggt gtagcgcctg tgctgacgcc tgccctgcgg atcttttgcc
    48241 gcaacagttg tactggttca gcaaaggtca gcaacacgat aaagctacca cgcataacat
    48301 tgctgattgc attgaatgtg gggcttgcgc gtgggtttgc ccgagcaata ttcccctggt
    48361 gcaatatttc cgtcaggaaa aagctgaaat tgcggctatt cgtcaggaag aaaagcgcgc
    48421 cgcagaagcc aaagcgcgtt tcgaagcgcg ccaggctcgt ctggagcgcg aaaaagcggc
    48481 tcgccttgaa cgacataaga gcgcagccgt tcaacctgca gccaaagata aagatgcgat
    48541 tgctgccgct ctggcgcggg tgaaagagaa acaggcccag gctacacagc ctattgtgat
    48601 taaagcgggc gaacgcccgg ataacagtgc aattattgca gcacgggaag cccgtaaagc
    48661 gcaagccaga gcgaaacagg cagaactgca gcaaactaac gacgcagcaa ccgttgctga
    48721 tccacgtaaa actgccgttg aagcagctat cgcccg
//
LOCUS       DAZKIV010000018        47598 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZKIV010000018 DAZKIV010000000
VERSION     DAZKIV010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47598
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.018"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..686
                     /gene="comR"
                     /locus_tag="ACHOT6_001063"
     CDS             54..686
                     /gene="comR"
                     /locus_tag="ACHOT6_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFP9946318.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLHLWPELVK"
     gene            complement(748..1287)
                     /locus_tag="ACHOT6_001064"
     CDS             complement(748..1287)
                     /locus_tag="ACHOT6_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFP9946319.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHOT6_001065"
     CDS             complement(1514..2818)
                     /gene="ndh"
                     /locus_tag="ACHOT6_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFP9946320.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3218..3760)
                     /gene="ycfP"
                     /locus_tag="ACHOT6_001066"
     CDS             complement(3218..3760)
                     /gene="ycfP"
                     /locus_tag="ACHOT6_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFP9946321.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3783..4808)
                     /gene="nagZ"
                     /locus_tag="ACHOT6_001067"
     CDS             complement(3783..4808)
                     /gene="nagZ"
                     /locus_tag="ACHOT6_001067"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFP9946322.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKTISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4819..5643)
                     /gene="thiK"
                     /locus_tag="ACHOT6_001068"
     CDS             complement(4819..5643)
                     /gene="thiK"
                     /locus_tag="ACHOT6_001068"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFP9946323.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5624..6265)
                     /gene="lpoB"
                     /locus_tag="ACHOT6_001069"
     CDS             complement(5624..6265)
                     /gene="lpoB"
                     /locus_tag="ACHOT6_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFP9946324.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6279..6656)
                     /locus_tag="ACHOT6_001070"
     CDS             complement(6279..6656)
                     /locus_tag="ACHOT6_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFP9946325.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6659..7018)
                     /gene="hinT"
                     /locus_tag="ACHOT6_001071"
     CDS             complement(6659..7018)
                     /gene="hinT"
                     /locus_tag="ACHOT6_001071"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFP9946326.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7352..9541
                     /gene="fhuE"
                     /locus_tag="ACHOT6_001072"
     CDS             7352..9541
                     /gene="fhuE"
                     /locus_tag="ACHOT6_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFP9946327.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGIYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGTPRNFSITGTYQF"
     gene            complement(9601..11034)
                     /gene="ptsG"
                     /locus_tag="ACHOT6_001073"
     CDS             complement(9601..11034)
                     /gene="ptsG"
                     /locus_tag="ACHOT6_001073"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFP9946328.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11329..12126)
                     /locus_tag="ACHOT6_001074"
     CDS             complement(11329..12126)
                     /locus_tag="ACHOT6_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9946329.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12137..13141)
                     /gene="holB"
                     /locus_tag="ACHOT6_001075"
     CDS             complement(12137..13141)
                     /gene="holB"
                     /locus_tag="ACHOT6_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFP9946330.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13138..13779)
                     /gene="tmk"
                     /locus_tag="ACHOT6_001076"
     CDS             complement(13138..13779)
                     /gene="tmk"
                     /locus_tag="ACHOT6_001076"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9946331.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13769..14791)
                     /gene="yceG"
                     /locus_tag="ACHOT6_001077"
     CDS             complement(13769..14791)
                     /gene="yceG"
                     /locus_tag="ACHOT6_001077"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFP9946332.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLVLGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14794..15603)
                     /gene="pabC"
                     /locus_tag="ACHOT6_001078"
     CDS             complement(14794..15603)
                     /gene="pabC"
                     /locus_tag="ACHOT6_001078"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFP9946333.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPAKR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15723..16964)
                     /gene="fabF"
                     /locus_tag="ACHOT6_001079"
     CDS             complement(15723..16964)
                     /gene="fabF"
                     /locus_tag="ACHOT6_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFP9946334.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGGGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17052..17288)
                     /gene="acpP"
                     /locus_tag="ACHOT6_001080"
     CDS             complement(17052..17288)
                     /gene="acpP"
                     /locus_tag="ACHOT6_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9946335.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17499..18233)
                     /gene="fabG"
                     /locus_tag="ACHOT6_001081"
     CDS             complement(17499..18233)
                     /gene="fabG"
                     /locus_tag="ACHOT6_001081"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFP9946336.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQS
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18246..19175)
                     /gene="fabD"
                     /locus_tag="ACHOT6_001082"
     CDS             complement(18246..19175)
                     /gene="fabD"
                     /locus_tag="ACHOT6_001082"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9946337.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19191..20144)
                     /gene="fabH"
                     /locus_tag="ACHOT6_001083"
     CDS             complement(19191..20144)
                     /gene="fabH"
                     /locus_tag="ACHOT6_001083"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFP9946338.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20212..21282)
                     /gene="plsX"
                     /locus_tag="ACHOT6_001084"
     CDS             complement(20212..21282)
                     /gene="plsX"
                     /locus_tag="ACHOT6_001084"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFP9946339.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSHAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21363..21536)
                     /gene="rpmF"
                     /locus_tag="ACHOT6_001085"
     CDS             complement(21363..21536)
                     /gene="rpmF"
                     /locus_tag="ACHOT6_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFP9946340.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21588..22109)
                     /gene="yceD"
                     /locus_tag="ACHOT6_001086"
     CDS             complement(21588..22109)
                     /gene="yceD"
                     /locus_tag="ACHOT6_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFP9946341.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            22308..22892
                     /gene="yceF"
                     /locus_tag="ACHOT6_001087"
     CDS             22308..22892
                     /gene="yceF"
                     /locus_tag="ACHOT6_001087"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFP9946342.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23004..23963)
                     /gene="rluC"
                     /locus_tag="ACHOT6_001088"
     CDS             complement(23004..23963)
                     /gene="rluC"
                     /locus_tag="ACHOT6_001088"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFP9946343.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24536..27721
                     /gene="rne"
                     /locus_tag="ACHOT6_001089"
     CDS             24536..27721
                     /gene="rne"
                     /locus_tag="ACHOT6_001089"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFP9946344.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSKETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27918..28871)
                     /gene="flgL"
                     /locus_tag="ACHOT6_001090"
     CDS             complement(27918..28871)
                     /gene="flgL"
                     /locus_tag="ACHOT6_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFP9946345.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRNQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28883..30526)
                     /gene="flgK"
                     /locus_tag="ACHOT6_001091"
     CDS             complement(28883..30526)
                     /gene="flgK"
                     /locus_tag="ACHOT6_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFP9946346.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNTGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30592..31533)
                     /gene="flgJ"
                     /locus_tag="ACHOT6_001092"
     CDS             complement(30592..31533)
                     /gene="flgJ"
                     /locus_tag="ACHOT6_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFP9946347.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31533..32630)
                     /gene="flgI"
                     /locus_tag="ACHOT6_001093"
     CDS             complement(31533..32630)
                     /gene="flgI"
                     /locus_tag="ACHOT6_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFP9946348.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVPLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32642..33340)
                     /gene="flgH"
                     /locus_tag="ACHOT6_001094"
     CDS             complement(32642..33340)
                     /gene="flgH"
                     /locus_tag="ACHOT6_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFP9946349.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYAGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33393..34175)
                     /gene="flgG"
                     /locus_tag="ACHOT6_001095"
     CDS             complement(33393..34175)
                     /gene="flgG"
                     /locus_tag="ACHOT6_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFP9946350.1"
                     /translation="MISSLWIAKTGLDAQQSNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34313..35068)
                     /gene="flgF"
                     /locus_tag="ACHOT6_001096"
     CDS             complement(34313..35068)
                     /gene="flgF"
                     /locus_tag="ACHOT6_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFP9946351.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35088..36293)
                     /gene="flgE"
                     /locus_tag="ACHOT6_001097"
     CDS             complement(35088..36293)
                     /gene="flgE"
                     /locus_tag="ACHOT6_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFP9946352.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKTGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36318..37013)
                     /gene="flgD"
                     /locus_tag="ACHOT6_001098"
     CDS             complement(36318..37013)
                     /gene="flgD"
                     /locus_tag="ACHOT6_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFP9946353.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNNGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37025..37429)
                     /gene="flgC"
                     /locus_tag="ACHOT6_001099"
     CDS             complement(37025..37429)
                     /gene="flgC"
                     /locus_tag="ACHOT6_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFP9946354.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37433..37849)
                     /gene="flgB"
                     /locus_tag="ACHOT6_001100"
     CDS             complement(37433..37849)
                     /gene="flgB"
                     /locus_tag="ACHOT6_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFP9946355.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38005..38664
                     /gene="flgA"
                     /locus_tag="ACHOT6_001101"
     CDS             38005..38664
                     /gene="flgA"
                     /locus_tag="ACHOT6_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFP9946356.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIARGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38740..39033
                     /gene="flgM"
                     /locus_tag="ACHOT6_001102"
     CDS             38740..39033
                     /gene="flgM"
                     /locus_tag="ACHOT6_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFP9946357.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39038..39454
                     /gene="flgN"
                     /locus_tag="ACHOT6_001103"
     CDS             39038..39454
                     /gene="flgN"
                     /locus_tag="ACHOT6_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFP9946358.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKSQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(39494..41029)
                     /gene="murJ"
                     /locus_tag="ACHOT6_001104"
     CDS             complement(39494..41029)
                     /gene="murJ"
                     /locus_tag="ACHOT6_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFP9946359.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41139..42062)
                     /locus_tag="ACHOT6_001105"
     CDS             complement(41139..42062)
                     /locus_tag="ACHOT6_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9946360.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42064..42711)
                     /locus_tag="ACHOT6_001106"
     CDS             complement(42064..42711)
                     /locus_tag="ACHOT6_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFP9946361.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42722..43306)
                     /gene="rimJ"
                     /locus_tag="ACHOT6_001107"
     CDS             complement(42722..43306)
                     /gene="rimJ"
                     /locus_tag="ACHOT6_001107"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9946362.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43542..44750
                     /gene="mdtH"
                     /locus_tag="ACHOT6_001108"
     CDS             43542..44750
                     /gene="mdtH"
                     /locus_tag="ACHOT6_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFP9946363.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44814..45461
                     /gene="grxB"
                     /locus_tag="ACHOT6_001109"
     CDS             44814..45461
                     /gene="grxB"
                     /locus_tag="ACHOT6_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFP9946364.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45595..46155
                     /locus_tag="ACHOT6_001110"
     CDS             45595..46155
                     /locus_tag="ACHOT6_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9946365.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46261..47307
                     /gene="pyrC"
                     /locus_tag="ACHOT6_001111"
     CDS             46261..47307
                     /gene="pyrC"
                     /locus_tag="ACHOT6_001111"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFP9946366.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTGSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            47381..>47598
                     /gene="dinI"
                     /locus_tag="ACHOT6_001112"
     CDS             47381..>47598
                     /gene="dinI"
                     /locus_tag="ACHOT6_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFP9946367.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWE"
BASE COUNT        11504 a        12897 c        11829 g        11368 t
ORIGIN      
        1 gtgattattt ttataataac ctttcattca caatcaccag gtacaacggc atcatggcaa
       61 ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc aaaagtgttc gacagggatg
      121 ccgcgcttga taaggccatg aaattgttct ggcaacacgg ttatgaagcg acttctcttg
      181 cggacctcgt cgaagcgacc ggagccaaag cgcccacgct atacgcggaa tttaccaaca
      241 aagaggggtt atttcgtgcc gttctcgacc gctatatcga tcgttttgcc gctaagcatg
      301 aagcacagct gttttgtgaa gagaaaagcg tggagtctgc gctggctgac tattttgctg
      361 ccatcgccaa ctgctttacc agcaaagaca ccccggcagg ctgcttcatg atcaacaact
      421 gcaccaccct ctccccagat tcaggagata tcgccaatac gttgaaatca cgccatgcga
      481 tgcaagagcg cactttgcag cagtttttat gtcaacgaca agcgcgcggg gaaatcccga
      541 cccactgtga cgtgacacat ctggcagaat tccttaattg tattattcag gggatgtcga
      601 tcagcgcacg cgaaggtgca tcgctggaaa aactaatgca gattgccgga acgactttgc
      661 atttatggcc cgaactggtg aaataagtcg caaaaaaagc ccctcagcct gcgcgaatga
      721 ggggccaaat tgcagagtac agccttgtta tactttgtta ttcaaaatca gttgcccatt
      781 actatctagc gggatctgcg tacccggatc gcggtccatg cggattttgc cctgctgatc
      841 gccaatctta taggtcacat cataaccgag cattttttct gacttgtcat acaccgtttt
      901 acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg agagagccct ggatctggtt
      961 accggcatat ccaccaccca gcgcccccac aacagtggcg acatctttac cgcgaccacc
     1021 gccaaactga tgccctatca cgccgccagc aacagcgccg agcaccgacc cggtaatgcg
     1081 attttcatcc tgcaccggtc gacgatgggt cactgtgacg ttgcgacact cctgacgcgg
     1141 tgttttaacc gtttccttga ttggggttgc agaaacaacc tgagcgtatt gcgggccacg
     1201 ttcaaacacg ttcagactgg ccactgccgc tacgcccagc gcagctgcga caccaatccc
     1261 gatacccgcc aacattgatt tattcacggg aacacctcct tctttgcagt tatgccaatc
     1321 atgcaagcga aggagaaaag cagctatcag acagaagatg aaaagcgcgg gaatggctgg
     1381 aaatacttaa tgattcggag gagtctgaag gaatgcctga tgcgacgctt aacgcgtctt
     1441 atcaggccta ccgctcgtac catttgtcca gggcggatga ggcgtttatg ccacatccgc
     1501 cagtgtacgt cgattaatgc aacttcaaac gcggacggat aacgcggtta atactcccca
     1561 ccagcatcat taatccggtt ttaaagtaac catgcagcgc aatctgatgc attcggtata
     1621 gcgagatata tacaaagcgc gcaattcgtc cttcaatcat cattgagccg cgcgtcaggt
     1681 tacccatcag gctaccaacg gtggagaagt tcgacagcga taccagcgaa ccatgatctt
     1741 tatactgata atttttcagc ggcttaccat tcatctgcgc cagaatgttg ttcattgcgc
     1801 aagtcgccat ctggtgtgca gcctgagcac gcggcggaac aaagccccct tccggacgcg
     1861 ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc gcgggtggtt tgcagcgtcg
     1921 gttccaccac cagctggttg atacggttag tttcaagacc accgatatct ttcaggaagt
     1981 ctggcgcttt gatcccggct gcccacacca tcagatcagc ctcaatatat tcgccatctt
     2041 tagtgtgcag gccgccttca tcagcactgg tgaccatggt ttgcgtcagc acgcgaacgc
     2101 caagtttcgt tagctcgttg tgggccgcag cagagatacg tggcggtaat gcaggcaaaa
     2161 tacgttctcc cgcttctacc agcgttacgt tcagggcttc gttggtcagg cctttgtaac
     2221 cgtagctgtg cagttgcttg accgcgttgt gcaattcagc ggagagttct acacccgtcg
     2281 cgccgccgcc gacaatcgca atgttcactt tgccatttgc gcccaggttg gcagagtatt
     2341 tcaggaacag attcagcatc tcctggtgga agcgacgcgc ctggtgcggg ttatcgagga
     2401 aaatgcagtt ctctttgacg cctggcgtat tgaaatcgtt agaggtgcta cccagcgcca
     2461 tgaccagggt gtcataggcg attttacgtt ccggaaccag cagttcacct ttctcgtcgc
     2521 gtagttctgc aatagtgatt gttttcgctt cacgatcaat atcaatgacg gaacccaact
     2581 ggaactggaa accatgattg cgcgcatggg ccagatagct caacgcatcg acgccttcat
     2641 caagcgagcc agtcgccact tcgtgcagca gcggtttcca caggtggctg tggttacgat
     2701 cgaccagcgt aattttggct tttttcttac gtcccagctt atgccccaac tgtgttgcca
     2761 tttccagccc accagcaccg ccgccgacaa tcacaatctt tttcaatggc gtagtcaacg
     2821 tgaccccctt aaatttatta accaattgtt aattaatggt tattaacata gctcttaatt
     2881 aacaacaggt tacagcaatg aaaatgttct tcgctattga gaataacatg aatggtgcat
     2941 tggtcatacc aaaattgatg ttaatcaagt tttgttgctg aaaagatagg cgactttacc
     3001 gggtgagatc aaaaaataga gtttattgtt tattatttga gcaaattcgg aagagtaata
     3061 tagggtgaag cgtatatcga gggcgtttgg tcgtcctgat aagaggcgca tcaggcattg
     3121 atgctcgact gtcggatgcg gcgtaaacgc cttatccgac ccacagaatc cggagcgcat
     3181 caggtaccgg caaggttacc tgatgcgacg agggcattta cccgagggtt ttgaacgctt
     3241 taatgcgctg taaatgcggg gagatattct taaatttgtg cgtctgctct tcgtcccaga
     3301 caatctcgta ataatgatgc aactcttcag atgtccgctg gctgttaagt gcttcatcat
     3361 tacgcgacaa aataaccaga caacgatcgc gattcttctc gcggaagttg gtcacacact
     3421 tagtggcaat atcggcatac tcttccgggc gatcaatctt gccttccatg ttctcataag
     3481 ggaacaaatt agggttgaag atcacctggc ggatatcgca aagaaaacca atccgttccg
     3541 cccagtatcc acccaggcca acgccgcaaa ttagcggacg ctcgtcaacg ttcagttgca
     3601 acattttgtc cacttcttta agcagatgct gcatatcatg tttcggatgc cgcgtactgt
     3661 agcttatcaa gcgtacatcc gggtcaataa actgcaattg taagactttc tcgtggttac
     3721 ccggactgtt agagtcaaaa ccgtgtaaat agataatcat cgcttcctca cataagccag
     3781 ggttagtgac ctgctttctc ttcctgccag cgttcgtgta actgattcag acgggtgctg
     3841 atcgttttcc agcgagccga gtccatcagt tcctgtcgcg aaaatgaacc tttatgatac
     3901 aaacgtgtaa cacgttctgc cttgatcggt gacagattat ctaacacgct gacggcccct
     3961 ttacgattat tgcagaccag gatcatatcg caacccgcat ccagtgatgc ctgcccgcgt
     4021 tcggcataac tgcccataat cgcggcacct tccatcgata aatcgtcaga gaaaatcacg
     4081 ccgtcaaaac ccagttcctg acgcaaaacg gttttcagcc agtagggaga accgctcgcc
     4141 ggacgcggat caacatcact gtagatcaca tgcgcaggca taatggcgtc gagtttattt
     4201 tcgcggatta aggaactgaa gaccgacata tctttagcgc gaatctccgc ttgtggacgt
     4261 ggatcgcacg gtgtttcttt gtgtgagtct gccgttactg caccgtgtcc tgggaagtgt
     4321 ttcccggtcg ttttcattcc ggcttcatgc ataccatcaa taaaccggct ggcaattgcc
     4381 agggcttttt gtggatcggc atgatatgaa cgctcgccaa tcgccgcgct gatatgcccg
     4441 acatccagca ctggcgcaaa gctgatatca atatccatag cgatcatttc gctggccatc
     4501 agccaacccg cctcttgcgc cagtttgccg ccctcttcca ttcccaacag cgcagcaaag
     4561 gattgtgctg ccggtaagcg ggtaaaacct tcgcgaaaac gctgcacgcg tccaccttcc
     4621 tgatctaccg ccaccaccag atgattgcgc gatgctgcgc ggatctggcg caccagttca
     4681 cgtaactggg caggatcatg atagttacgc gtaaagagaa tcagccctcc caccagcgga
     4741 tgcgccagta tttcacgctc ttccgcgtcc agttcgtaac cttcgacatc caacattact
     4801 ggacccacac tgctctcctt attgttttat taatagctgc cgccaggtgt catcggccag
     4861 cctgataaat tgttgatcgc cggtttgtcg ccagcggtac tcaaaccacc ctgctttgag
     4921 catcagcagc cagggaaacc atcgcctgac ctgacgccat aattgcgccg gataaatctt
     4981 cgcgcgagtg gcatagtcat tgaccaattg ccggtgctgt tcagtatttt ccacccacac
     5041 cgccgccagt tccagcgcga tatcaccatc tccggcatac tcccagtcga tgagttttaa
     5101 ccctgacgcg ctatgcacta aatttccggc gtggacatcc atatgcaatg gacttaagcg
     5161 taaaggccgt ggttcccgcg ctttgcgcag acgttttaac attcgcagcc aacccactgt
     5221 ccgccgcgcc ggatcgcttt gctgccagta cagttccagt aacggcaaca gcgttattcg
     5281 ccagccaaaa cgtggttgtt gatgtagata atacagcaag cctgccagtt cgttggtatc
     5341 cggcaaatac gtttttaccg cgccgggcag atagtcgact accatccagt cacggagata
     5401 taaatgcggc ttcggtgcaa tgcttgcggg tagttgtgat aaagcccgat actggcgcaa
     5461 gaacgcggac tgcggcgcat caggatcgtg cggctgacgc acaacaaaac gctggccctg
     5521 atgttcaatg agaaaactcc cgccactaag cccactatta aacgtcgtga cgggatgaaa
     5581 ctgcgggaaa aagcgcgaca gcaattcgtc gcgcatgatg ggattattgc tgcgaaacgg
     5641 cacctttacc tgaccagata atttcgcccg tctgcaccag catcagctgc atttgtaggg
     5701 tcggagcgtt aacgttgcca gaggcgctgg agtacagcac gtaatgagcg ccgacattgc
     5761 gggcaatgcc tatggcttta ctacgggtac ctaaactgtc ctgcggcgac aaacctaact
     5821 gttgcttagc catcgacagc tgctgggcgg aaaccagggt aaatttcccg ttattagcca
     5881 gcgcatttcg cagcgtttcg gtcgcttctg cggcattcag cgaaccgtta gtacggttgt
     5941 taacgctatc aaccagcagg acgctacccg cagtcacccc gtcagcccca agcatcttac
     6001 tgaccatcgg ctgcattgcg ccattccagt cataatggcg aatatgcggc gcaggcggtg
     6061 ccgtttgatc ttcgtgctca attgggcctg gctgctgcgg gatcgtcggc accgagggca
     6121 ctgtggggac aggctgttgt ggctcggctg gttgttccgg cgctggtttc acttcttcta
     6181 ccggtgcagg ttcacgttgc cccacacacc cggcgagaaa catcgccagc gcggtaatca
     6241 aggcgtagcg actcattttt gtcatcaaga ttcacccctt acaaatatag ataaagtctg
     6301 actttgtgcg cccccagaaa attggcgctg ccgtacagcg ttaccgccga atgtgcggga
     6361 atgctaacgc tgcgtggcct ttccagagga tgcatctcca gccctctggc gtcataccag
     6421 taaaaacgat aatgaacggt aacgggttct tgcctttcgt tataaagcgt tgaggatgct
     6481 gaaggttgaa tatcagacgt cgaaaggacg ggcttttctg cactgatgcc cgcagccaat
     6541 aaagatgact ccatcaccag cgattgctca tcattcaccg gaatttccgg atgtgaacga
     6601 cagcccacca gcagtaacaa cgccagagac accagcccaa agcatccttt tctcatcgtt
     6661 acagaccttt atgcgccagc attggtccca gcggacggcc acccaacaag tgcatatgga
     6721 tgtggtaaac ctcttgtccg ccatggcggt tggtattcat gatcagacga tagccatctt
     6781 cggcaatacc ttcttgctca gcaatttttg ccgctacggt gatcatgcgt cccagcgcct
     6841 gctcatgctc agctgagacg tcgttcacag tcggaatgag gatattcgga atgatcagaa
     6901 tatgcgttgg cgcttgcggc gaaatatcgc gaaacgccgt taccagatca tcctggtaga
     6961 cgatatcgga ggggatctca cgacgaataa ttttgctgaa tatagtttct tctgccacga
     7021 cattttcctt tttcaaatag ccatgcgttg cgccatgcta cgccgcacca cactgcgagt
     7081 atagagtatg gtcgagttaa acctgctctt tcaaccttaa ctcacgattt cttaagcaaa
     7141 aaatcgtttt gctgccgtat atatcgccat tattcccatt tctgcctgcg aatgcgctga
     7201 aaagacattc tctgtttggt ttacacgaca aatatgaatg cgtatatttc tcatttgcat
     7261 ttacaaacaa aattattcgc acactaaata taatcgctca tcaccctcct ccggcatgag
     7321 cctgacgaca acataaacca agagatttca aatgctttca acacaattta acagggataa
     7381 tcaatatcaa gccatcacca aaccgtcact acttgccggt tgcatagcac tggcactatt
     7441 accttctgcc gcttttgctg caccagccac tgaagaaacg gtgattgttg agggttcagc
     7501 cacagctcca gatgatggcg aaaatgatta cagcgtaacg tctacctctg cgggtaccaa
     7561 aatgcagatg actcaacgtg atattcctca gtcggtcact attgttagcc agcagcggat
     7621 ggaagatcag cagttacaaa cgctgggcga agtgatggaa aacacgctgg ggatcagcaa
     7681 aagtcaggcg gattccgatc gtgctcttta ttattcccgc ggattccaga tcgataacta
     7741 tatggttgat ggtatcccca cctattttga atcgcgctgg aatctgggcg acgcactttc
     7801 tgatatggca ctctttgaac gcgtagaagt agtgcgtggc gcgacaggac tcatgaccgg
     7861 gacgggtaat ccatctgcgg caattaatat ggttcgaaaa cacgcgacca gtcgtgaatt
     7921 taaaggcgat gtctcggcgg aatacggtag ctggaacaaa gaacggtatg tggcggattt
     7981 acaaagccca ctcaccgaag acggtaaaat ccgcgcgcga attgtcggcg gctaccagaa
     8041 taacgactca tggctggacc gctacaacag tgaaaagacc ttcttctcgg gcattgtcga
     8101 tgctgattta ggcgatctta cgacgctttc agccggttac gaatatcagc gcattgatgt
     8161 taatagccct acctggggcg gtttaccgcg ctggaatact gatggcagca gcaacagtta
     8221 cgatcgtgca cgcagtaccg cacctgactg ggcgtacaac gataaagaga tcaacaaggt
     8281 ctttatgacc ctgaagcagc ggtttgctga tacctggcaa gcgacactga atgccaccca
     8341 ctctgaagtc gaatttgaca gcaaaatgat gtatgtcgat gcctatgtaa acaaagcgga
     8401 tggtatgctg gttgggccat acagtaatta tggacctggc tttgattatg tcggcggcac
     8461 cggttggaac agtggcaaac gtaaagttga tgcgctggat ttgttcgctg acggtattta
     8521 tgaattgttt ggtcgtcagc acaatctaat gtttggtggc agttacagca aacaaaacaa
     8581 tcgttacttc agttcatggg ccaacatctt cccggatgaa attggcagtt tctacaactt
     8641 taatggcaat ttcccacaaa ccgactggtc accacagagc ctggcacagg acgataccac
     8701 acatatgaaa tcgttatatg ctgccactcg agtcaccctt gccgatccgc tgcatctgat
     8761 cctcggcgca cgttatacca actggcgggt tgatacgctg acttacagca tggagaaaaa
     8821 ccacaccacg ccttacgctg gtctggtgtt tgacatcaat gacaactggt cgacctacgc
     8881 cagctatacc tctattttcc agccgcaaaa tgatcgtgac agttcaggca aatatctggc
     8941 tccaatcacc ggtaacaact acgagctggg tctgaaatcg gactggatga atagccgtct
     9001 gaccaccacg ttagccatct tccgtattga gcaggataat gtcgctcagt ccaccggtac
     9061 acctatcccc ggcagcaacg gcgaaaccgc ctataaagcg gtggatggga cagtcagtaa
     9121 aggggtggaa tttgaactca acggcgcaat taccgacaac tggcagctga catttggcgc
     9181 aacgcgctat attgcagagg ataacgaagg aaacgccgtt aatcctaatc tgccacgcac
     9241 cacggttaaa atgttcacca gctatcggtt gcctgtcatg ccagagttga cagtcggcgg
     9301 tggtgttaac tggcaaaatc gcgtgtatac cgacaccgtg acaccgtatg gcaccttccg
     9361 cgccgagcaa ggtagctacg cgctggtgga tctcttcacc cgctaccagg tgacgaaaaa
     9421 cttctcgtta caggggaacg tcaataacct gttcgacaaa acctacgata ccaacgtgga
     9481 aggttctatc gtctacggca caccgcgtaa tttcagcatt accggcacgt atcaattctg
     9541 aataacacct gtaaaaaagg cagccatctg gctgccttag tctccccaac gtcttacgga
     9601 ttagtggtta cggatgtact catccatctc ggttttcagg ttatcggatt tagtaccgaa
     9661 aatcgcctga acaccagaac cagcaaccac tacgcccgct gcgcccagtt tcttcaggcc
     9721 ggcctgatcc actttagaca catcagcaac gctgacgcgc agacgggtaa tacatgcgtc
     9781 gaggttagta atgttttctt taccaccaaa tgcagcaacc agagccggtg ccatttcgct
     9841 ggtacctgtc gcttttgcat cttcagtcgc gtcttcacga cccggcgttt tcagatccag
     9901 tgctttaatc agcacgcgga agatggtgta gtaaacaatc gcataaccga taccgacgat
     9961 cgggaacagc cacagtttgc tgctgttacc agacagaacg atgaagtcga tcagaccgtg
    10021 cgagaacgac gtaccgtcac gcatccccag aagaatacag attgggaatg ccaggcctgc
    10081 cagaatcgcg tggatgatgt acaggatcgg cgcaacgaac atgaaggaga actcgatcgg
    10141 ctcggtgata ccggtcagga acgaggtcag cgccgcggaa atcataatac cgcccacttt
    10201 cgcgcggttt tctggtttag cagagtgcca gatagcaatt gcggcagctg gcagaccgta
    10261 cattttgaac aggaagccac cagacagttt acccgcagtc gggtcacccg ccatataacg
    10321 cggaatgtcg ccgtggaaaa cctgacctgc tgcgttggtg tattcaccaa tctgcatctg
    10381 gaaaggtacg ttccagatgt ggtgcagacc aaacggtacc aggcaacgtt cgatgaaacc
    10441 gtaaatgcca aacgcaacta ccgggttctg gtaagcagcc cactgagaga aggtctggat
    10501 tgcagaacca atcggcggcc aaatgaagga cagcacaacg ccagtaaaga tggcagccag
    10561 gccagaaatg atcggcacaa agcgtttacc ggcaaagaag ccaagatact caggcagctt
    10621 aatacggtag aaacggttaa acatgtacgc tgcgatcgca ccggagataa tccctccgag
    10681 tacgccagta tccgccaggt gtttagaggc gatttcttca gcaggtaaat gcagtaccag
    10741 tggcgcaacc acggccatgg ttttaaccat gatgccatag gcaacaactg cggccagcgc
    10801 ggatacgcca tcgttattgg taaagccgag ggcgacaccg atcgcaaaaa tcagtggcat
    10861 gtttgcaaag acggaaccgc ctgcttctgc cataacatgc gatacaacgg cgggcagcca
    10921 gctgaaattc gcggaaccga cgcccagcag aatacctgcg ataggcagta cggataccgg
    10981 cagcatcagc gatttaccga ccttttgcag gttagcaaat gcattcttaa acataattga
    11041 gagtgctcct gagtatgggt gcttttttta cgttctcacg cgtggcaagg ggggagagcc
    11101 tcgccgtgta cagggcatct aagcgccctt tatttattac acagagtaaa ataattcagt
    11161 gccaatttgt ttgacggcta tcacgtttca acttcagatc attggagaag ttgcacattt
    11221 tgacaaaaca tatgtcacaa tatttcaaac gcggttattc accgcccaat tttcggcggt
    11281 gaactttact cgttttagcc attaattacg agctttaaaa aaactcattc aacggatgga
    11341 ttgaaggcgg gaagcgtcga tgtgaaacag acgggcgaag ttatcggtgg ttacctgcgc
    11401 cagttcttca acggcaacac ctttcaacac agccatgtat tctgcaacgt cacgaaccat
    11461 cgcaggttga ttctcttttc ctcgatgcgg taccggcgca aggtaaggtg agtcagtttc
    11521 caccagtaat cgatccaggg ggacataacg cgcagcatcg cgcagttgct ccgcattacg
    11581 gaaggtcaca atgccggaaa aggagatgta aaatccgaga tccagtaatt tacccgccgt
    11641 ttctctgtcc tctgtaaaac agtgtagtac gccaccgcaa tccgtcactt tttcttcgcg
    11701 cagaattgcc agcgtatcgg cacgggcgtc acgggtatgg acgataaccg gcttgttcag
    11761 ttcacgacca atctggatat gatggatgaa ggactcttgc tgacgtactt tagtttccgg
    11821 tgtgtaataa taatccagcc cggtttcacc cagcgctaca acaccctctt ctgccgccag
    11881 acggcgtaaa tcttctacat cgtagggatc attctggttg agtggatgca cgccacaaga
    11941 aaatacgacg ttgtcacgtt cgcctaccag atcccgcata tgtaaataac ccggtaatgt
    12001 tgtggcgact gccagacaaa atttcacatc gcgtgcggcg gctttcgcca gaacgtcatc
    12061 cacgtcctta tgcaaagatt cataatccag accatcgaga tggcagtgtg agtcgactaa
    12121 aaacatgatg tctctcttaa agatgaggaa ccggtagcac aacgcccggt tgcaggtaat
    12181 gctcaatacg cagtaaaaga tcggtgatga gaagctcgcg gttgatgcct gtgacagaca
    12241 ttaactgttc acgaatgtgg caaacatccc ccagtatagc ctgcaggcgc gagggagaaa
    12301 gatggtttgc cagttcggcg accaggcccg gcacatcaac attggtcacc tgcgcagcgc
    12361 catgatggcg ttttagcgca tccatcagca acgttgccag ccagtgtaaa cgcgccggag
    12421 cttgttcatg attaagggcc gccagcagcg aataccagtc gcccgatggc acgctatatg
    12481 ccaacgcctg acacaatgtt tcacgagcct gccagttatc tccctgaaac aacgccagtg
    12541 ccgcgccagg cgaaccggcg cttaagcgca atgcggcaag taatgcatcc tgtgacattg
    12601 tcacttcgcg tgaaagccag gtcacggcgt actgttctgg aggcggcgca aggtaatgta
    12661 accgacaacg actacgtaat gttgccagta aacgttcagg ctcgcgggta gccaggaaaa
    12721 accaggtttc tgctggtggc tcttcaagcg ttttcagcaa tgcgttagcc gcggcgtcgg
    12781 tcagtaaggc agcatcggtt acccagacaa ctttcgcacc acctaggcgt gcgtgctcat
    12841 tcagtttttc ggtgacctca cgtaccgcat caatgcccag cgtatttttc cctttttcgg
    12901 gagccagggt gtagtaatcg ggatgcgtgc cagcctgcat caactgacat ccacgacagt
    12961 gaccgcaact tttgtgtccc tgcggttgtt ggcagagtaa ataacggctc agggcgtaga
    13021 ttaaagcatc atcgcccatg cccggtaacg cctgaatgag tagcgcatgg tgacctcttc
    13081 cggcctgata gctggctacc agtttttcga agtcaggtcg taaccatgga taccatctca
    13141 tgcgtccaac tccttcaccc agtgggtcac ggtagtgcgg attgcatcca tcacggcctc
    13201 cagcggctgg gtggcatcaa tggtatgaat acttttatct tgtgcagcca gttccagata
    13261 gcgggcgcgg gtgcgattaa agaaatcgaa agactcttgc tcaatacgat ccagctcgcc
    13321 gcgcgcacgc gcgcgtttta agccaacttc cggggtaaca tcgagataga gcgttaagtc
    13381 ggggcgaaaa tccccgagaa cagcatcacg cagtgttgcc agcatatgtt ggtcaatacc
    13441 acgtcctccg ccctgatacg cctgagtgga gagatcgtgg cgatcgccaa tcacccaggt
    13501 gccgttagcc agagctggtt tgatgaccgt ttctaccagt tgaacgcgcg cggcataaaa
    13561 catcagaact tcggctttat cggtaatgac ttcatcgcct accgatttga tatccagcac
    13621 caggcttctt aacttttcgg caagttgcgt accgccaggt tcccgagtga aaaccatgtc
    13681 gcggataccc agttgctcaa gcgtctcaac caccacatta cgcgcggtag ttttgcctgc
    13741 gccttccagc ccctcaatga cgatatactt actgcgcatt tttttcctta agcactttca
    13801 gataatcctg cacagacttg ttatggctgg caagattggt attaaacgtg tgaccacctt
    13861 taccatcggc cacaaaatag agatacggcg tttttgccgg atgcgcagca gccttcagcg
    13921 aatccgcccc cggcgtagct atcgcacccg gcggcaaacc ggtaatggta taggtgttat
    13981 acgctgtcgg cgtttccagg tctgcacgag aaagtttgcc attataacgc tctcccatcc
    14041 cgtaaatcac ggttgggtcg gtctgcaggc gcataccaat gcgtaaacgg ttgataaata
    14101 ccgaggcaac ctgatcgcgt tcactggcaa cggcggtttc tttttcgata attgatgcca
    14161 tcgtcaccag ctgattttta tctttataag gcagaccgtc cgcacgccct tcccaggcgc
    14221 tatcgaccgc tttcaccatt ttcttgtgcg ctcgcttgag taacgcgaca tcggtggtat
    14281 tggcggtata catccaggtg tctggccaga accaaccttc aatccactcc gggttttcca
    14341 gttcaagtgc ctgcgctacg gtggcgtact tatcgtcact cagcgtgtgc ttgatatacg
    14401 gagcctcacg caattgcttg aggtagtcgc tcagacgcat cccttctacc agtcgcagcg
    14461 ggaactgtgc ttctttaccg ctttccagca atttcagcat ctcgcgcacg gtcatctgcg
    14521 gtgtaaagcg gtaagtcccg gctttaaagt gagaaagatc cggttcgata cgcagcaacc
    14581 attgaaaaac ccgtgggcga ttgatgatct tatcggcata aagctgttca ccaagcacca
    14641 gacgtccggt ccctggcttc agggtaaata tcgtctcttc tttgataagc aatttgctgt
    14701 cggcaagatg gcgaaccttc cagacgccca caccagcggc gatacccagt accaccagca
    14761 ataacaagat aatcaataac acttttttca tgactaattc gggcgctcac aaagtggggc
    14821 taaatattca tataacgttg ctgacgaaaa ggagacatcg ccacaggcac gtacgggcat
    14881 cactggcatt aacgcattac aaataaccat ctcatctgcc tgcaacgcct cttccagaga
    14941 ggcttgcact tcgacaagct gataagagga ttgtgccagc aaacggatac agaattgtcg
    15001 cataatgccg ttaacacctg cctgatccag tcgcggcgta taaactacat tgcccttccg
    15061 ccagaacaaa ttagccgcac agcattccgt aacccaccct tcgctgtcaa ggaccagcgc
    15121 ctcatcagcg tttgtctgct caagatgaga gcggatcaat acttgctcaa gccgattaag
    15181 atgtttaata cctgcaagat gaggattgcg ccccagccgc accgggctta gcgccaacgt
    15241 catcccttcg ttacgcaaac ggtcgtaatg tgcaggataa accgtaacgg agagaatccg
    15301 ctttgccggt ccactgttca atgtgctgta ccctcgcccg ccactaccgc gactgatcac
    15361 gactttcagc acaccatttt gctgttctgc tgccagcgtt ttcatctctt gttcaagctg
    15421 aggccagaag tcacagaaaa tcatcaaccg ctgacaagca tcctgtagtc gctggatatg
    15481 cgccgataac aaactgactt taccgtcgat aactctggcg gtggtaaaac aaccatcacc
    15541 aaactgcgtt gcccgatcgc ttactgccag cgattcctgc ttatgaccgt taattaagaa
    15601 cataccggct ccttatcaga gaattgttag tgtggcagca tgttcactac ggaacaagtc
    15661 ggaataaaag ctaagaaaaa aggcccgcaa gcggaccttt tatatgggtg ggaaatgaca
    15721 acttagatct ttttaaagat caaagaacca ttagtgccac cgaagccgaa ggagttacac
    15781 agcgtgtatt ccattccgct gacctgacgt gcttcatgcg gtacgaaatc cagatcgcaa
    15841 ccttcatccg ggttatccag gttgatggtt ggcggaacag cctgatcgcg cagcgccagg
    15901 atggagtaga tagactctac tgcccccgcc gcacctaaca ggtgaccggt catagatttc
    15961 gtggagctta ccaacacacg gcttgcagct tcaccgaaga tggctttcac cgcctgcgct
    16021 tcagctttat cgccagccgg cgtagaagta ccgtgcgcat taacgtagcc aatctgactc
    16081 gcttcaatgc ctgcatcacg cagagcattt gccattgcca gagctgcgcc tgcgccattt
    16141 tctggcggtg acgtcatatg ataagcatcg ctgctcatac caaagccgac gagttcagcg
    16201 taaattttcg caccgcgttt tttcgcgtgt tcgtactctt ctagtaccag cataccgcca
    16261 ccatcgccca gtacgaagcc atcacgctct ttatcccacg ggcggctcgc cgcttgcggg
    16321 ttatcattgc gggtagataa tgcacgtgcc gcgccaaaac caccaacacc cagcggcgta
    16381 ctggctttct ctgcgccacc tgcaaccatc acgtcagcat cgccatacgc gataatacgc
    16441 gcagcatggc caatgttgtg cacgccggaa gtacaggcgg tcgcgataga gatgcttggg
    16501 ccacgcaggc catacatgat agtcagatga cctgccacca tgttcacaat cgttgacgga
    16561 acgaagaatg ggctgatctt acgtggacca ccgttcatca gagatgtgtg gttttcttcg
    16621 atcagtccga ggccgccaat cccggagcca attgcggcac caatgcgggt tgcgttctct
    16681 tccgttattt caaggccaga atcctgcatg gcctgaacgc cagcgacaat tccatattga
    16741 atgaaggcat ccatcttgcg ctgttctttg cgcgagataa tgtcctcaca gttaaaatcc
    16801 tttactaagc cagcaaattt cgttgcatag gcgctagtat cgaaatggtc gattaggctg
    16861 atgccactct gaccggcaag cagagctttc caggtagact ctacggtatt gccgacagga
    16921 gacaacatgc ccagtccggt cacaactaca cgacgcttag acacgtttgt cctccaggga
    16981 gggaaaaaat gattctagtg ggacaaaaag ataaaactca ggcggtcgaa cgaccgcctg
    17041 gagatgttca cttacgcctg gtggccgttg atgtaatcaa tggcagcctg aacggtggtg
    17101 attttctcag cttcttcgtc cggaatctca gtatcaaact cttcttccag agccattacc
    17161 agctcaacgg tgtcaagaga atccgcgccc aggtcttcaa cgaaagaagc attgttggta
    17221 acttcttcct gcttaacgcc cagctgttcg ccgataattt tcttaacgcg ttcttcgata
    17281 gtgctcatac tcttaaattt cctatcaaaa ctcgctttcg cgatggtttt cgtagtgtat
    17341 aaaatgttga aaaatttgca actaaatccc ggcaggtctt accacgattt tacgttattt
    17401 tgaggcctta ccccccaata acgcaaatca ttttgcacta attattactc ataaccacgc
    17461 agactgcgcg caacatgagc attttgtgca aatcgcggtc agaccatgta catcccgccg
    17521 ttcacatgca aagtttcacc cgtgatgtaa gctgcttcgt cggatgccag gaatgcaacc
    17581 gcgttggcga tttcctgtgc gccgccgagg cgacccgcag gaacctgcgc caggataccc
    17641 gcacgctggt catcgctcag cgcacgtgtc atgtccgttt caataaagcc cggagcaaca
    17701 acgtttacag taataccgcg tgacgcaact tcgcgcgcca gtgatttact gaagccgatc
    17761 aagcccgctt tcgccgcagc gtagttggcc tgaccgccat ttcccatggt accaaccaca
    17821 gaaccgatag tgataatacg accatgacgc tttttcatca tagcgcgcat taccgctttt
    17881 gacagacgga aaacagatga aaggttggtt tcgataatat cgttccactc ttcatctttc
    17941 attcgcatta acaggttatc acgagtgata ccggcattat tgaccaggat atccacttca
    18001 ccaaattctg cgcgaatttt ttccagaaca gattcgatag atgccgggtc ggtcacattc
    18061 aacatcagac ctttgccgtt agcacctaaa taatcactga tcgactgagc gccattttca
    18121 ctggtcgcag tgccaataac tttcgcgcca cgggctgcga gcgtttcagc aattgcgcgg
    18181 ccaattccgc ggcttgcacc ggttaccagt gcgatttttc cttcaaaatt catgattttc
    18241 ctcttttaaa gctcgagcgc cgctgccatc gctgaaggtt cgttcagcgc cgaggcggtc
    18301 agggtgtcga caatgcgttt cgtcaggcca gttagcactt tgcccgggcc gacttcatag
    18361 agatgttcta cgccttgcgc tgccatgtac tcaacagact tcgtccactg aaccgggtta
    18421 tacaactgac gtaccagtgc gtcacgaatg gcatcaccat tggtttcgca tttcacatca
    18481 acgttattca caacaggaac tgttggtgcg ttaaaggtga ttttcgctaa ttctactgcc
    18541 agtttgtcgg ctgctggttt catcagcgca cagtgagacg gtacgctcac tggtaacggc
    18601 agcgcgcgtt ttgcgcccgc agctttacag gcagcgccag cacgctcaac cgcctcttta
    18661 tgaccggcaa taaccacctg tcccggagag ttaaagttta ccggagaaac gacctgacct
    18721 tctgcagctt cttcacacgc tttcgcaata gacgcatcat ccagaccgat gattgccgcc
    18781 atagcgcccg tgccttctgg tacggcttct tgcatgaact tgccgcgcat ctcaaccaga
    18841 cgcaccgcat cagcgaaatc aatcacgcca gcgcaaacca gcgcggagta ttcccccagg
    18901 ctgtgaccgg ccatcattgc cggtgcttta ccgccctgct gctgccatac gcgatacagc
    18961 gcaacagatg cagtcaacag cgctggctgg gtttgccagg ttttattcag ttcttcagct
    19021 ggcccctgct gggtcagcgc ccacaggtcg tagcccagcg ccgcagaagc ttcagcaaac
    19081 gtttcttcga caattggata gctcgccgcc atatcagcca gcattccaac ggtttgagaa
    19141 ccctgtccag ggaacacaaa tgcaaattgc gtcatgtttt aatccttatc ctagaaacga
    19201 accagcgcgg agccccaggt gaatccaccg ccaaaggctt caagcagaac caactgcccc
    19261 ggcttaatgc gcccgtcgcg tacagcttca tccagcgcgc acgggacaga ggccgcagag
    19321 gtattaccgt ggcgatccag cgtcaccacg acattgtcca tcgacatacc gagttttttc
    19381 gccgttgcac tgataatacg caggttagcc tgatgcggaa ccagccagtc cagttgagaa
    19441 cggtcaagat tattcgccgc cagcgtctca tcaacgatgt gcgccagttc cgttaccgca
    19501 accttgaaga cttcgttgcc cgccatcgtc agatgaattg aattctctgg attcacgcgg
    19561 tcggcgtttg gcagcgtcag caattcacca taactaccgt cggcatgcag atgggtggag
    19621 atgattcccg gctcttcaga ggcagccagc accgcagcgc ccgcgccatc gccaaaaata
    19681 ataatagtcc cacgatctgt tggatcgcag gtgcgcgcca gtacatcgga accgacgacc
    19741 agagcatact tcaccgcccc agatttcacg tattgatcgg ctacgcttaa tgcataggtg
    19801 aaacctgcgc aggctgctgc aacgtcaaat gccgggcaac ctttaatgcc caacatgctt
    19861 tgaatctgac aagctgcgct cgggaaagcg tgcgtagcag aagtcgttgc cacaacgatc
    19921 aggccaatct ggtctttctc aatgcccgcc atctcaattg cgcgtgtcgc cgcttcaaag
    19981 cccatggttg aaacggtttc gtttggcgcg gcaatgtggc gttcgcggat accggtacga
    20041 gtgacaatcc actcgtcaga ggtgtccacc attttttcca aatcggcgtt tgtccgcact
    20101 tgttcgggca gatagctgcc agtaccaata atcttcgtat acatgtacgc tcagtcactt
    20161 ttcggttata taccgtcact tgcaaactgc gagttcgctg gcagcgttct gctaccgcag
    20221 agttccgctt ttgccaccgt ccagcagctc aaaaccagct gggtatacag attccaggcg
    20281 agcggcaatt cgctgaggaa cttgtcgctg caccgcctgc actgcctgtt caatcgcgac
    20341 cgcaaaagct cgctgattgg ctgcaccatg actttttatc accgtgccgc gcaatcctaa
    20401 cagacaggcg ccgttatact ggtcggggtt gaggtgactg aatcgcctcg tcaggctctt
    20461 ttgtagccaa cgctttaata acagtagcca ccacgaccgt tttttccctt caccctgaga
    20521 tttcagcaga gaaaggaaca tcctgacaac accttccatc gtctttaacg tgacatttcc
    20581 tgtaaagccg tcacaaacca gcacatctgt cttgccagtt aacaactcat tggcttcaag
    20641 atagccgata tagttgatag aagggattgt tttaagcacc gctgaggcat cccgaatact
    20701 gtcgagaccc tttacttctt cttcaccaat attgagcaac gccacgcgag gattgggaat
    20761 ttccaccacc tcttcagcca gaactgagcc cataatggca aattgcacca acattgtgct
    20821 gtcacaatcg acgttggccc ctaaatcgag gaccaccgtt ttgccctttt gctgatgtgg
    20881 taataccgtc accagcgccg gacgctcaat cccctccagg ggcttgagta ataattttgc
    20941 cagccccatc agtgccccgg tattaccggc actgacacag gcttgcgctc gaccttcttt
    21001 caccagctcc agggccatac gcattgaact cccacgactg gcgcggatag cgtgcgaagg
    21061 ccgggcatca ctggcgataa ctgactgcgc aggaataatc tgcagacgcg aacgttgttc
    21121 aaagtcagct ttagcaagta atggcgtgat ggcgtcggga ttgccgacta aaagaagagt
    21181 gagttgcgaa ttagaattca gtgcctgcaa tgctgcaggc actgtcacgg aagggccaaa
    21241 atcccctccc atgacatcta acgccagggt tagacgtgtc aaggtatcgt cgccgcccgg
    21301 tttggtcttt ccccagttgc ctggggaaaa tcctcactaa gcttcatcac gcagacgcgt
    21361 gattacttag cgatgacctt gcggccgcgg tagtaaccgt cggcagtgat gtggtgacgc
    21421 aggtgttttt caccagaagt tttgtctaca gacaggctgg tgactgcggt cagcgcgtca
    21481 tgggaacgac gcatgccacg tttggaacgg gttggtttat tctgttgtac ggccatggac
    21541 cttactcctc aattacggtt tatccccgat ccaacgggga gcaccaatta cttacgcttt
    21601 aagctggcta atacggcaaa tgggtttggc ttttgcactt cttcaggcag ttcaccaaag
    21661 accatgtccg cttcggacac ttcacagtgt tcagaatcat gcaccggaac tactggcaag
    21721 gcgaggatga tttcatcttc aaccattgca agcagatcga tttcaccgaa ttcgttaacc
    21781 tcaatcggtt catacgcttc cggcagtgct tcagcctgtt cgtctgaacg cacaggacta
    21841 aaacaatacg ttgtgtagac ctgatgagta aacggcttcc cgcaacgctg acactcgagc
    21901 gttaccgtca ccttcgcatc gccgtttaac actgcgagac gttggttatc gatagcgaac
    21961 gacatggagc attccacatc actgtccaca ctgactacgg attcggcgac gcgctcaacc
    22021 tgatcagggg tatagatacc ctggtaatca aggcgttttt gagccgtacg aaccggatcg
    22081 agagtcaggg gtaattttac cttttgcata gggcgcgcat attaactttg taacgtcata
    22141 gagtcaaaga aaaaggcagc ctgtggttgc cttttgccaa taattcgcac acattgcggg
    22201 ttactgcttt attttcgttc agtgctgata acgaacatca acctgcaaaa cgaagcgcat
    22261 agtttaaaat ggctatcatc aatacgctat atctgatgga aaaaaatatg cctaaactta
    22321 ttttagcctc cacctcgcca tggcgtcgcg ctcttctgga aaaactgcaa atctcttttg
    22381 aatgtgcagc accagaggtc gacgaaaccc cacgcagcga cgaatcaccg cgacagttgg
    22441 tgcttcgact ggcacaagaa aaagcgcaat ctctggcgtc acgttatccg gatcatttaa
    22501 ttattggttc agaccaggta tgtgttcttg atggcgaaat caccggtaaa ccgttaacgg
    22561 aagaaaatgc ccgtctgcaa ttacgcaaag ccagcggcaa tatcgtcacc ttctatactg
    22621 gactggcgtt gtttaattcg gcgaatgggc atctacaaac agaagtggag ccttttgacg
    22681 tccatttccg tcacctgagc gaggcggaga ttgataatta cgtgcgtaag gagcatcccc
    22741 tgcactgcgc gggtagcttt aagagtgaag gatttggcat tacgctgttt gagcgtttag
    22801 agggacgtga ccctaacacg ctggttggtt taccgctaat cgcgctgtgt cagatgttac
    22861 gtcgggaagg gaaaaacccg ctgatgggat agttgtttgc gccggatgcg gttacgtcgc
    22921 atccggcaat cggtgcaggc ggcctgatgc ggcgtaaacg ccttatcagg ccaaccgccc
    22981 attatcaggc ttatatgctt gtattagcgc gcgttacgca gcttttgcaa acaacgcttc
    23041 aaaccttcat ccatcggcgc ttcgatacgc atcacttcac cggtccccgg atgggtaaac
    23101 ttcaacgctg cggcgtgcag gaacagacga tttaatcccg tgcctgcttc agtgagctgt
    23161 ctgtcaaatt cacggtcacc gtagcgatcg tcaaaggcaa tcggatgacc cgcatactgt
    23221 gtatgcacac ggatctgatg agtacgtccg gttaccgggc tacaacgcac cagggtggca
    23281 aatgcatagc gttcttccac tttaaagcgt gtttccgacg gtttgccttc ctgactgaca
    23341 cgcacgatac gttcgccgct ttgcagaata tttttcaata acggcgcttg aacgctcttc
    23401 acatgcgact gccactgacc gcgcaccagc gccaggtaat ctttttgcat tcctttttca
    23461 cgtaattgct catgcagaga acgcaacgcc gagcgttttt tcgctaccag caacacacct
    23521 gaagtgtccc ggtcgagacg atgaaccagt tcaaggaacc gcgcttccgg gcgcaacgcc
    23581 cgcaaacctt cgataacgcc gaagcttaaa ccactgccgc catgtaccgc cgtaccggaa
    23641 ggtttattca gcaccaggat atgatcatct tcatataaga tgacgtccgc cagcgccgcc
    23701 accttttgca gatgtggcga aaccgcctct tcttcccgct cagcaacgcg aaccggtggg
    23761 atacgcacct catcacccgc ttcgagttta tattcaggct taatacgttt tttgttcacc
    23821 cgcacttcac ctttacgcaa aatacggtaa atcatacttt ttggtacgcc tttcaattgg
    23881 gtacgcaaaa agttatcgat acgttgcccc gcttcgtcag cggtgatagc aacaattttt
    23941 acggatggag tctctgtttt catggtgggc gattctaatt agccaacagg attcgcgcca
    24001 ctcatttttc tatgcttata tttagttttg caccttatta cttcactacg tgatcacttt
    24061 attgatggtt attaaaccaa tcaccagcaa gaagtgaaaa aactgtgagt aagcgggtga
    24121 taaatggtaa aagtcatctt gctataacaa ggcttgcagt ggaataatga ggccgtttcc
    24181 gtgtccatcc ttgttaaaac aagaaatttt acggaataac ccattttgcc cgaccgatca
    24241 tccacgcagc aatggcgtaa gacgtattga tctttcaggc agttagcggg ctgcgggttg
    24301 cagtccttac cggtagatgg aaatatttct ggagagtaat acccagtctg tttctttgat
    24361 aattgcgctg tttttccgca tgaaaaacgg gcaaccgaca ctctgcgcct ctttgagctg
    24421 acgataaccg tgaggttggc gacgcgacta gacacgaggc catcggttca cacccggaaa
    24481 ggcgttactt tgcccgcagc ttagtcgtca atgtaagaat aatgagtaag ttacgatgaa
    24541 aagaatgtta atcaacgcaa ctcagcagga agagttgcgc gttgcccttg tagatgggca
    24601 gcgtctgtat gacctggata tcgaaagtcc agggcacgag cagaaaaagg caaacatcta
    24661 caaaggtaaa atcacccgca ttgaaccgag tctggaagct gcttttgttg attacggcgc
    24721 tgaacgtcac ggtttcctcc cactaaaaga aattgcccgc gaatatttcc ctgctaacta
    24781 cagtgctcat ggtcgtccca acattaaaga tgtgttgcgt gaaggtcagg aagtcattgt
    24841 tcagatcgat aaagaagagc gcggcaacaa aggcgcggca ttaaccacct ttatcagtct
    24901 ggcgggtagc tatctggttc tgatgccgaa caacccgcgc gcgggtggca tttctcgccg
    24961 tatcgaaggc gacgaccgta ccgaattaaa agaagcactg gcgagccttg aactgccgga
    25021 aggcatgggg cttatcgtgc gcaccgctgg cgtcggcaaa tctgctgagg cgctgcaatg
    25081 ggatttaagc ttccgtctga aacactggga agccatcaaa aaagccgctg aaagccgccc
    25141 ggctccgttc ctgattcatc aggagagcaa cgtaatcgtt cgcgcattcc gcgattactt
    25201 acgtcaggac atcggcgaaa tccttatcga taacccgaaa gtgctcgaac tggcacgtca
    25261 gcatatcgct gcattaggtc gcccggattt cagcagcaaa atcaaactgt acaccggcga
    25321 gatcccgctg ttcagccact accagatcga gtcgcagatc gaatccgcct tccagcgtga
    25381 agtccgtctg ccgtccggtg gttccattgt tatcgacagc accgaagcgt taaccgccat
    25441 cgacatcaac tccgcacgcg cgacccgcgg cggcgatatc gaagaaactg cgtttaacac
    25501 caacctcgaa gctgccgatg agattgctcg tcagctgcgc ctgcgtgacc tcggcggcct
    25561 gattgttatc gacttcatcg acatgacgcc tgtacgccac cagcgtgcgg tagaaaaccg
    25621 tctgcgtgaa gcggtgcgtc aggaccgtgc gcgtattcaa atcagccata tttctcgctt
    25681 tggcctgctg gaaatgtccc gtcagcgcct gagcccatca ctgggtgaat ccagtcatca
    25741 cgtttgtccg cgttgttctg gtactggcac cgtgcgtgac aacgaatcgc tgtcgctctc
    25801 tattctgcgt ctgatcgaag aagaagcgct gaaagagaac acccaggaag ttcacgccat
    25861 tgttcctgtg ccaatcgctt cttacctgct gaatgaaaaa cgttctgcgg taaatgccat
    25921 tgaaactcgt caggacggtg tgcgctgtgt aattgtgcca aacgatcaga tggaaacccc
    25981 gcactaccac gtgctgcgcg tgcgtaaagg ggaagaaacc ccaaccttaa gctacatgct
    26041 gccgaagctg catgaagaag cgatggcgct gccgtctgaa gaagagttcg ctgaacgtaa
    26101 gcgtccggaa caacctgcgc tggcaacctt tgccatgccg gatgtgccgc cagcgccaac
    26161 gccagctgaa cctgccgcgc ctgttgtagc cccagcaccg aaagctgcac cggcaacacc
    26221 agcaactcct gcacaacctg ggctgttgag ccgcttcttc ggcgcactga aagcgctgtt
    26281 cagcggtagt aaagaaacca aaccgaccga gcaaccagca ccgaaagcag aagcgaaacc
    26341 ggaacgtcaa caggatcgtc gcaagcctcg tcagaacaac cgccgtgacc gtaatgagcg
    26401 ccgcgacacc cgtagtgaac gtactgaagg cagcgataat cgcgaagaaa accgtcgtaa
    26461 tcgtcgccag gcacagcagc agactgccga gacgcgtgag agccgtcagc aagctgaggt
    26521 aacggaaaaa gcacgtacca ccgacgagca gcaagcgccg cgtcgtgaac gtagccgccg
    26581 ccgtaacgat gataaacgtc aggcgcaaca agaagcgaag gcgctgaatg ttgaagagca
    26641 atctgttcag gaaaccgaac aggaagaacg tgtacgtccg gttcagccgc gtcgtaaaca
    26701 gcgtcagctc aatcagaaag tgcgttacga gcaaagcgta gccgaagaag cggtagtcgc
    26761 accggtggtt gaagaaactg ccgctgccga accaattgtt caggaagctc cagctccacg
    26821 cacagaactg gtgaaagtcc cgctgccagt cgtagcgcaa actgcaccag aacagcaaga
    26881 agagaacaat gctgataacc gtgacaacgg tggcatgccg cgtcgttctc gccgctcgcc
    26941 tcgtcacctg cgcgtgagtg gtcagcgtcg tcgtcgctat cgtgacgagc gttatccaac
    27001 ccagtcgcca atgccgttga ccgtagcgtg cgcgtctccg gaactggcct ctggcaaagt
    27061 ctggatccgc tatccaattg tacgtccgca agatgtacaa gttgaagagc agcgcgaaca
    27121 ggaagaagta caagtgcagc cgatggtgac tgaggtccct gtcgccgccg ctgtcgaacc
    27181 tgttgttagc gcgccagttg ttgaagaaat ggctgaagtc gtagaagccc ccgtaccggt
    27241 tgccgaaccg caaccggaag tggttgaaac gacgcatcct gaagtgattg ccgccgcggt
    27301 aactgaacag ccgcaggtga ttaccgagtc tgatgttgcc gtagcccagg aagtcgcaga
    27361 acacgcagaa ccggtggttg aaccgcagga agagacggca gatattgaag aagttgccga
    27421 aactgctgag gttgtggttg ctgaacctga agttgttgct caacctgccg cgccagtcgt
    27481 cgctgaagtc gcagcagaag ttgaaacggt agccgcggtt gaaccagaga tcaccgttga
    27541 gcataaccac gctaccgcgc caatgacgcg cgctccggca ccggaatatg ttccggaggc
    27601 accgcgtcac agtgactggc agcgccctac ttttgccttc gaaggtaaag gtgccgcagg
    27661 tggtcatacg gcaacgcatc atgcctctgc cgctcctgcg cgtccgcaac ctgttgagta
    27721 ataattagct caaagtaatc aagccctggt aactgccagg gcttttttat ttcatctttg
    27781 aatcaacata ttaaaattca tcaaatggca tccttgctaa ccaacaatgc aaaataggca
    27841 aaaaaaatgc ccgccccggc gatcccagag caggcagaca aaacataccc agtttcatga
    27901 tatgttttaa agcgagctta tttgttgagc tggaagagcg acaatccctg catatcggta
    27961 aatgctttat acgatgcctg caatgccgtt tgctgcatga tgtaagatga aatagttgca
    28021 ttccagtcca catcaaccag atcgctcatc tgctgcgttt gccctaaagc gcgatcgcta
    28081 cctaatgaat ccagcgactc cagttcgttc agctgcgtgc ctaattccgc gcgaacggta
    28141 aggacattat tcagcgagtt tttcagtcca cggttggttt tatccaatgc cgcagcggcg
    28201 atttctttat ccgcttcgct atccgcgacc ggcgttttca gggctgcgat ggcgctatcc
    28261 agcatggcaa aaagattggt ttcagaagcg ctaccgtctg gttccgctac cgcgttactg
    28321 gtaatactgt cgaaaatttt gtcgcccgta tgtcctatca ccatcgaacg cgaagcatcg
    28381 acctgttgcc taatactttc tgctccaccg acgtatttcc ctttctcttc gctaaacggc
    28441 gcagtctctg ttttataacc ggcaaaaatg tagcgcccgt taccgtcagt ggtgttcgcc
    28501 agattcagca actggttacg aagcccctgg atatccgtag ccagcgaggc ccggtcatcg
    28561 tcactcaaag tgccattgct ggcgtagaca attttttcct gagcattctg gatagcagtg
    28621 gtgacctggc taagtacact ctcttccagt gacacttttt gagtggcgaa agtacgcgcc
    28681 agcgtgtact ggctgttttg cgcctgtgcc tgggagagaa caaccgcttg tgatgctgca
    28741 atgggatcgt cagaagggtt aacgactcgc ttacctgtcg acatctgctc gccgtacttc
    28801 atccattccg cctgagaatt ggtgatgcca cgcatgtttt gctggtacat catctgggta
    28861 ctgaaacgca tcttatctcc ccttagcgaa tgttaatcag cgcatcaaaa atcgcgtttg
    28921 ccgtctgcag aacctgcgca ttcgccaggt aatactgctg aaaacgttgc agatttccgt
    28981 actcctcatc gagattgaca ccggaaatcg actgctgctg attggaaagc tgcgtcacca
    29041 cattaccttg cgtggcgcta ctggttttca acgtcgcggt tttattaccg atatcactca
    29101 ctaacgaggc ataagcgtcg ttaaaggatt tcgcaccgcc caccgtttta ctgttgcttt
    29161 gcagatccag cagggcctga ccgttgcggt tatcgctatc acccgcatct tcttcgctcg
    29221 ccatcgctat tttcgcttca tcggtgatta atacatccat gttgacgata gcgtcactta
    29281 ctggtttcag cgtgaagctg tcgttaacag caggcgttcc tgtaaacgtc aactccagac
    29341 catcaaatgc cactttaccg ttggcatccg gcgtcaccgt aaaagtggta ttactggcaa
    29401 ggcgggtgac ctgccactga ttattatcga acgagatttt gtaatcagtc gccagtaccg
    29461 cggaggcatc agttaccgtg gcaccgatcg caacgtcacc tgtgtttttc gtgttttgca
    29521 gaaccgcggg cttaccgata gcaaagaaat cttcaccagc atcgccgttg gcatcaaatc
    29581 cggctttgtg ttggctgttg aaagcctcgg caaatgccag cgccagttgt ccaagcgtat
    29641 tacgcgtctg gtccagatcc tgagaacgga atgtcagaat gccgcccagc gacccggtat
    29701 tcagtaattt ctccgggatc tcaatattgc ctgccgtccc atcaacgtaa gcgacagtcg
    29761 tacgagaagg gtcggcgctg gaaggaactg ccgccagttg ccgcgccgta cttccctgaa
    29821 ccagtgagta accattggcc atcgtgatgt tataagtacc gccatcctga acgctgactt
    29881 ctacgccaac aatctggttt aattcgctca ccagttgatc gcgttgatcc agcagattgt
    29941 taggtgacgc ccctgccccc acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa
    30001 tttgtttagc gtaattgttg atctgatcaa cgctggcacc tatcgcgata ttgacctgtt
    30061 tgtcctggtc gcgcagatat tgatcggtgg ttttaaactg attcaccaac ccttctgatt
    30121 tcccaatcag cgcctggcgc gctgccgggt cttccgcgtt actcaccagc gtttgcaggc
    30181 tggtgaagaa atcctgcatc tgtgttgcca gcgaagaggt actggtggag agcatattgt
    30241 cgattttcga catctgctca tagcgggcag tcaggccgct actttgcgtc tgcgccgcac
    30301 gtaactggtt ggtaataaaa gcatcatact cacgctgcac accagaaacg tagacaccat
    30361 tgccaaccca gccgccagcg cccaacgtgc tattggcctg cgccataata gtggtttggc
    30421 gggtatatcc ggcaacgtta tagctggaga tattattact tgccgtattt aacgccgcct
    30481 gggccgcgtt cagtccgctc atggcgttat tgatcaagct ggacatggag gttccttata
    30541 agccttcaat tacagagtat tatcggcagc gacccgccgg acttgagtta ttcagaacag
    30601 attatcaatg ttcatgctgt aggttttgct caccttgtcg cttatcgatt tcatctgctg
    30661 gatcatgttg gtgagtttac gggcatagtg aggatcggta gcataccccg cgtcctgtag
    30721 ggcctgcgcc ccctgctccg cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg
    30781 tgttaacagc ccaacgtaat ccgacaatgc ttccagatac gagctgtaga cccgaaactt
    30841 cgcttttact ttcttcgctt cgccgttttc atattcagtc gtggtgattt cagtaactgg
    30901 ccctttccag ttgccagagg ctttgacacc aaacaggtta tagctcggct cgccgttttc
    30961 gcggcggatt tgccgttgcc cccaaccaga ttccagtgcc gcctgagcga ggatcaaatg
    31021 atgtggcaca ccgctttgct ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa
    31081 tgctttactg tcacccggca gcgaatcatc gtagttacgt ggcacggcct tttgcaccag
    31141 ctgcgaaagc gcctgatttt gataacgcac cacggtttcg agcgggaatt tcatcggtgc
    31201 tgctggcgtg gactcctctg gcaatggttg ttctggcgtc atctgtttaa ccatcatctc
    31261 tgcaagcccc agacctttgc ccgccgtcat ctgttgggca atttgctggt catacatact
    31321 ggtatacaga cgagtgtgct cgctgctgaa caggccatct tttggtaaag cgtcgcgcat
    31381 gcttttcaac atcatctgca cgaacatccc ttccacctgg cgggccaccg gacggatatt
    31441 tgccgccgga tcttcgcccg ctttcgcctt tagttcgttg agtgattgcg catcccaggc
    31501 cgcacttgcc agtagtttgc tgtcgctgat catcagatga tttccagttt tgcccgcaga
    31561 catcccgcac tttgcattga ttgcagtatg gacatcagat ccatcggcgt agcgcccagc
    31621 gcattgagcg cgcgcaccac gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa
    31681 ccgccgctct ggcgtaaatc gatctgcgtt tgtggagtaa ccacagtctg tccaccacca
    31741 aacggtgtat ctggctggct gacattggcc tgacgattga ctgttactga gagatttccc
    31801 tgcgctaccg cgcagctgtc gaggggcact tcgcgattca tcaccaccga accggtgcgc
    31861 gagttaatca ctactttagc gtcctgcggg gtgacattaa cctgcatatt ctggatatct
    31921 gcaaggaaac ggacctggga actgttgcca cttggtacgc gcacctgaat agtccgcgca
    31981 tctaacgcgg tggcgctgcc atatccacgc acgcggttga tggtgtcagc gatttgctgc
    32041 gccatgctga aatcttcgtc gttaagttgc aaattaaggg tattcccgac gccaaactgg
    32101 ctgggcaatt cacgttcaat aaccgcacca ttggtgatcc gtccaccgtt cagttggttc
    32161 acctggacac tgctaccgcc agcggaggct cctgcgccgc caaccagaat attgccctgc
    32221 gccagcgcat acacctgact gtcaacgccc ttaagcggcg tcatcaacaa cgtacctcca
    32281 cgcaagcttt tggcatttcc catggaagaa accaccacat cgatggtttg cccctgacgt
    32341 ccaaacggag gaagtgacgc tgtcaccatt accgcagcga cgttttttag ctgcatattg
    32401 gtgcccgtcg gaacggtaat tcccagctgt gagagcatgt tattaagcgt ttgtgtggta
    32461 aacggcgtct gggttgtctg gtcaccggtg ccatccagcc ccaccaccag accatagcca
    32521 atcagtgagt tttgccttac cccctgaaca ctggtgagat cgcgaatacg ctcagcctga
    32581 gccgccgtcg taaccagtag aagaattaat gcagagagaa atttaatcac cacagcctca
    32641 cttacattgg cgacaggtta aggaagaaac gctgcaacca gcccatattt tgcgcttcgt
    32701 taatgtagcc attgcctgcg tattcaatgc gcgcatccgc cacctgagta gacggtacgg
    32761 tattgctgcc gctgatagtg cgtggattaa ccacgccaga gaagcgaata aattcggtac
    32821 cctgattaat ggcaatctgt ttttcaccca ccacatgcag gttgccgttg accagtacct
    32881 ggtcaaccgt caccgtcaac gtgccgctaa aggtattgct ggcattggcc ccgccctttc
    32941 cgttgaacgt gttaccaccg gaggcttcga catcggcacg agcgttacca aacagcccct
    33001 gcaaatagcg cggcacagta tcaaagccaa aattagtttt accgtcacgg ctggcattcg
    33061 cagaggagct tttgctggcg ctgacgttct cctgcaacac gatggtcagc gtatcgccaa
    33121 tattgcgtgg tcgacgatct tcaaacagcg gttgatagcc atagttaatc ggctgagcag
    33181 actggaaaat agaaccgttg gcgacgggcg tcggaccggg aaccggttgt gcactggtcg
    33241 ccccctgcac cagcggcgtg gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca
    33301 agctggaaat ggcataagta tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc
    33361 agtaaaccgg tgaacctgcc accggttaag ccttagagtt gcgtcagttt ttgcagcatc
    33421 tgatcggtgg tggacaccgc tttactgttg atttcgtaag cgcgttgcac ctgaatcata
    33481 ttgaccagtt cttccgccac gttgacgtta gacgtttcaa catacccttg atacagcagt
    33541 cccgcgccgt tcaggcccgg cgtgctttcg ttcggtgcac cagaggattg cgtttcggtg
    33601 tagaggtttt cgccaatgct ctccagcccg gtgtcattca taaaggtggt gagattgagc
    33661 tgcccaacct gaaccggagc tgcctggcct tgttgggtta cgctgaccac gccatcacga
    33721 ccaatggtga tacttaacgc attcgccgga atggtgatcg ctggctgcac ctgaaaacca
    33781 ccagccgtca ccagctgccc gttctgatcc acctggaaag agccgtcacg ggtataggct
    33841 gatgaaccat ctggcaacat cacctggaaa aagccctgcc ctttaatcgc gacatctttg
    33901 ctgttgttgg tctgcgacag gtttccctgg ctgtgtaagc gttcagtggc gaccgggcgt
    33961 acccccgtgc cgatttgtaa tccggagggt aaggtggttt gttcggaaga ctgtgccccc
    34021 ggctggcgaa tggtttgata aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg
    34081 ttagtactga cgtttgccag gttgttggca atgacgtcca tattgctttg ctgggcgtca
    34141 aggcccgttt tggcgatcca taatgaactg atcataaaat gtcctgtagt ggattgctga
    34201 caagcagagc agaagcacgc gacagtgtga atttcccctc accctaaccc tctccccaaa
    34261 ggggcgaggg aatagtccgc gcgcttttat tgcaatgtca tcaatcgttc aattaactca
    34321 tcgacagcag ttggttggca cggcctgcgt tatcatcgac gctgctgatc accttcatct
    34381 gcatttcaaa acgccgcgcg ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt
    34441 tactgccttc cagaaccccc gacatcacac gcaaggttgg atctgcctgc agtatcggcc
    34501 cacgcgtggc ctggctttct gcgcttaaac gaaaaatgcc gtcgtcaccg cgttgcactt
    34561 cgctgcccgt ggctttcacc agtttcagac gccccactgg cgcaaccgta tttgccggat
    34621 cgcccggatt gagcgccgag attgtgccat cggcagcaat agtgatttcc gccccttccg
    34681 gcacagcaat tggccccgcc tcgcctatca ccggatgccc ctgaattgtc agttgcccgg
    34741 tgggatcaac ctgaatgctg ccattacgcg tatacccttc gctgccgtca gcggtctgca
    34801 cggccagcca gccatcctgc tgcaacgcaa cgtccagcgg gcgcgaggtg taatccatct
    34861 tgccgggcgt catgtctgca cccggtgttg acgccgttac cagcgtgcgc gtaggcagtg
    34921 aaagcccttc caccggcact gcacgcaagg cgtttaactg cgcgcgaaaa ccgggcgttg
    34981 aggcattggc cagattactg gcggttaccg cctgttgatt cagtgtctgg ctggctgctc
    35041 ccatcgcagt ataaattgcg tgatccattg agctatcccg tcagcgatta gcgtaagtta
    35101 accagcgtgt tgaggatctg gtcctgggtt ttgatggtct gggcgttaga ctgatagtta
    35161 cgctgggcaa cgatcatatt gaccagttct ttactgagat cgacgttgga cgcttccagc
    35221 gcaccgttgg tcagggtgcc aaagtttccc gtcccggctg tccccaacag cgccacgcca
    35281 gaagattgcg tcgcagacca gacgttgtcg ccttcggatg ccagaccttc gttgttggca
    35341 aagttcgcca gtacaatctg ccccagcagt tgggtttgtt cgttggaata gttgccgaca
    35401 accgtaccgt catcattgat ttgataactc accagatcgc ccggtttgta gccgttctgg
    35461 gtggttgcca caatattgtt agcgccggta ttttgctgca tggagttgag gaagctcagg
    35521 ctaaacgtag cgggatctgc gccgttaatt gcgccagtgg taatattctc tgttggattt
    35581 gaggtcagaa cgccattggc attaaacacc agcttcattg caggctcggc tgtacctgtt
    35641 ggatcactgc tatcctgggt gtagacctgc cagttattat ccccggtctt cacaaagtac
    35701 acgctcatgt catgagcatt accctgacta tcgaaaacag tcaccgaacc ttttttgtta
    35761 tagctatccg cattgctggc gctgaatggc gtaacagtag gaagcggatc actggaattc
    35821 aggttgatct gcatcgacgc cgtggtggta gttttcgctg ccatcagggt attcgggatc
    35881 gaaatattgg tcggattcgc cccttgctga atagtcggcg gcgtaccggt tgccgggtaa
    35941 cccgtcagct gtaaaccttg catattcacc aggttacgat tttcatccag cttaaattgt
    36001 ccgttacggc tgtagaacac cgaaccgttg ctgtctacca gacggaaaaa accattctgg
    36061 ctgatagcaa cgtccagacc acgcccggtg ttggtggtcg tgccatcggt aaagtcctga
    36121 gtgataccgg caacttttac ccccagtccc actttcgaac cggcaaacat atcggcaaaa
    36181 gaagccgtgc ctgttttaaa gccgtaggtg gcggagttgg cgatattgtt gccaataaca
    36241 tcgaggttgg tggcggcagc gtttaatccg ctaaccgctt gagaaaaggc catgactgac
    36301 tcctgaaatg tgaaggctta aattatctgc cgtacttcgt cgagggtggt ggtgccgtaa
    36361 gtgccgagat ccagcgtatt accgttgttg ccgcggatca caccctgcac cagagcaaac
    36421 tgcagcggct gggcaaccag ttgtgtacca ccgttactgg cgctaatcgc tacattgtaa
    36481 gaaccgttcg gcgcagtgct gccgtcagtc aacgtaccgt cccaggtgaa actgtgaact
    36541 ccggcggtca gttcaccaat atcaatggtg cgcacaaccg caccattttt atcggtgatg
    36601 gtggccgtaa ctttgtctgc cgcctgttgc agctcaacac caaacggcgt ggtcgtggtc
    36661 acagctcctt cttcactgcc ggttcccgca agaacagtgg tgccggggat catcacgccg
    36721 tgaccgatca ggttactggc ctgtaacgac tggctgttat caatctgtcc ggaaatagat
    36781 ccgagcgtgg tattgagttt ttcaatcccg ctgaccgtgc tgatttgtgc caattgcgac
    36841 gtcagctcgt tgttttccat tggattggtc gggtcctggt ttttcagctg cgccaccagc
    36901 aaagtcagaa aactgctttg taaatctgcg gcgttgctgc ccgtgagcga actactgctg
    36961 gtggtactga cgccggtatt tgtcggatcg gtggtggtta ccgcaatgga catagctttc
    37021 tcctttattg accgagcgta agggttttca gcatcatact tttcaccgtg ttgagcactt
    37081 caacattggc ctgatagctg cgtgacgccg acatggtgtt aaccatctcc ccgacgacat
    37141 caacgttcgg catttttacg tagccctttg catccgccag cggattaccc ggttcataaa
    37201 ccagtttgtc cggggcctga ctttctataa catcggcaac ctttacgccg cctgtcgcag
    37261 cacctggtgc agcgttaacc tggaatacca cctgttttgc ccgatatggc tgtccatcgg
    37321 gaccggtcac gctatcagca tttgccagat tactggccgc cacgttcagg cgctgggact
    37381 gggcagttaa cgccgacccg gcgatatcaa aaatattcag cagtgccatc cgttaatttc
    37441 cgctctgtaa aacgttcatc atgcctttga tttgcccgct caacgcgcta aggctcatct
    37501 ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg atccatatcg acggtattac
    37561 cgtcaagcga aggctggtcc ggaatacggt attgcagttc tgcggtagga ggcgtcagcg
    37621 cctgcgccgg aatgtgttgc gttgaggtca tcgtcagtgc aaccacactg gttgcatccc
    37681 gtccacgttg catgactttt ttaagttcac tggcaaaatc gatatcgcgc gcctgataac
    37741 caggggtatc ggcattggcg atgtttgctg ccagcacttc ctgacgctgg gcgcgcagat
    37801 tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt atcgagcata tctcctccgc
    37861 aggtatcaaa attctgccat cagcttaaat gcctttacgc gcgcgttatc ggcggaataa
    37921 acgcaaaatg ggtcgctatt tatgccgttg atgaccattg cggacaggta caattcacgt
    37981 tgtagaaatg gctggggggc gaaaatgctg gcaataaaac gtagcgtggc gatcattgcg
    38041 atactgttca gtccgttaag tgcggcgagc aatctcacat cgcaattgca aacctttttt
    38101 agcgcccagc tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat
    38161 ctactaccgc cgtgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc
    38221 aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtacag
    38281 gccacaggaa attatgtggt tgccgcgatg cccattgcgc ggggaggaaa gctggaagct
    38341 ggcaatgtca aactgaaacg cggacggctg gataccctgc caccgcgtac ggtgctggat
    38401 atcaatcaac ttgttgatgc cattagcctg cgcgatctat cacccgatca acctatccag
    38461 ttaacccagt ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc
    38521 agcggtgatg ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca
    38581 cagaatgcgc gggtgcgcat ggtatcggga caggtagtca gcggcgttgt tgatgcagat
    38641 gggaatattc ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa
    38701 gcaacacatg ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg
    38761 cctctgaagc ctgtaagcac cgttcaaccg cgcgaaacca ctgacgcgcc ggtaacgaac
    38821 agccgggcgg caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa
    38881 gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa
    38941 ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc
    39001 aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct
    39061 cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca
    39121 tctctctatg gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc
    39181 actgctggcg acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa
    39241 tagcgttgat attagtcaac gctggcagga aattactgtg aaatcgcagc aactacgcca
    39301 aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct
    39361 ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac
    39421 aacccatcgc ggcggtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg
    39481 ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg
    39541 ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc
    39601 gccagggcat ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc
    39661 cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg
    39721 gagtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg
    39781 ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca
    39841 tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc
    39901 gggaataaaa gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt
    39961 aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga
    40021 acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca
    40081 acaggaaaca aagacgcaac ccccagtcca tcaaacggtt gtattcatca tgattgccac
    40141 tggcaaaact tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac
    40201 cggacggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa
    40261 gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga
    40321 tcgccggtcc catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg
    40381 gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc
    40441 cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa
    40501 acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg agcaaacgcc ggaatcgaga
    40561 agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat
    40621 agggaaaggt aatctttagt agctggctgg tcagggcaaa tttgtcggca gtgtcagcga
    40681 agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa
    40741 ccgccagcgc gagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt
    40801 caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt
    40861 cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg
    40921 ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg
    40981 aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa
    41041 tccagaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct
    41101 ggattatggt gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc
    41161 cagatcttgt caacgatacg ttgcgccagc actgcctgtt cgccggcggt ttgcggaact
    41221 gtctgatttt gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgctca
    41281 agagtgctct gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc
    41341 cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca
    41401 cgctgacttc cggcacggcg atgcatacag gtggtgatct gcaaaggacc agccgaaaaa
    41461 tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga
    41521 gaggctttgc cacccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc
    41581 aacgtgaaat aaagatcgtg tggcccgacg ctattgctac gatgtttatc cattcttaac
    41641 gaggctgcgg tggcgagttg cgtttttaac tctccgtaga gtggtgcgaa acgacggtta
    41701 aaaccgacca tcaacgtcag ttttttccgc gccgccagtt ccaccagccg ttcagcatcg
    41761 cgcagatttt ctgccagcgg tttatcgaca cagacatgta cccccgcatt gagtaacgta
    41821 ctgaccacgt caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg
    41881 gcaaggctgg ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct
    41941 ttcgcgcgcg taggcgacca ggctccttgt aacgtccagt cagacgctgc cgccagcacc
    42001 ggtaaccacg ctttttgcgc aatgccacct aatcccacta cgccgatacg taattttttc
    42061 actttaatct cccagatggg ccagcaacga atcaagacgc tgtttcagtt ctgccacttc
    42121 gatttccagg gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt
    42181 caccgccggc tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt
    42241 acccggttcg cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc
    42301 cagcgtcgac tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg
    42361 cagttcacct ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttccgc
    42421 tgcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac
    42481 ccgattacca aaaccgctca ctgtgcgtag ataatgacgt ttgaccagat tatccagctg
    42541 ttcctgcact tcggattcac tcagattcat gaccggttca cggttcgttt tctgattaca
    42601 ggccgtgact acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc
    42661 cagcaagcaa ccaatcacgc gagcttcaag ggcagtaagt tgatatttca tgagattctc
    42721 cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt
    42781 gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca
    42841 gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt
    42901 gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct
    42961 gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagcca cgaacaacat
    43021 tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataag ccaaagtaga
    43081 aagctgaacc ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg
    43141 gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg
    43201 cgtaataatc cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt
    43261 ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc
    43321 ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg
    43381 aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa
    43441 gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg
    43501 cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtatcgcagg
    43561 cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct
    43621 ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca
    43681 tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg
    43741 gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg
    43801 ccgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc
    43861 tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac
    43921 taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg
    43981 gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct
    44041 gcgccacagg ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg
    44101 catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg
    44161 acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag
    44221 tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcaccctct gccgttaaat
    44281 ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga
    44341 gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa
    44401 gcatgatgcc ggtgggcatg gtcagcggcc tgcaacaact tttcaccctg atttgtctgt
    44461 tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg
    44521 acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg
    44581 ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag
    44641 agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc
    44701 agtttagtca gaaacgcgcc gcgcgtcgtt tgctggaacg cgacgcctga tttgatactc
    44761 ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc
    44821 tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa
    44881 atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg
    44941 tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca
    45001 tggatatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt
    45061 cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgccaacaaa ctgctgttgc
    45121 cgcgttttgc caaatcggca tttgatgagt tttctactcc cgccgcgcgc aaatatttcg
    45181 tcgacaagaa agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc
    45241 tgattaagaa tatcagcgat gatttgcgtg cgctggacaa actgatcgtc aaaccgaacg
    45301 ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga
    45361 cgctggtagc cggaatcaac tggccaagcc gcgttgctga ttaccgcgat aatatggcga
    45421 aacagacaca aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag
    45481 cctgtattcg cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg
    45541 acacatgctg cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac
    45601 aagtttttat ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc
    45661 acccaataca ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc
    45721 tcaaaagata tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc
    45781 agccaaattg gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac
    45841 atgaactccc tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc
    45901 gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg
    45961 gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca
    46021 ttacgcaatt actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc
    46081 gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt
    46141 ccgtttacgg attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa
    46201 cgtttccgct tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta
    46261 atgactgcac catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc
    46321 cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct
    46381 atcgtaatgc ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc
    46441 caacgtattc ttgatgccgt acctgccggg cacaatttca ccccattgat gacctgttat
    46501 ttaacaggtt cgctggatcc taatgagctg gagcgcggat ttaacgaagg cgtgttcacc
    46561 gctgcaaaac tttatccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt
    46621 gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat
    46681 ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc
    46741 gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc
    46801 accaaagatg ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact
    46861 ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg
    46921 tattgtttac ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc
    46981 agcggtttta atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa
    47041 gagagcagtt gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct
    47101 accgtctttg aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc
    47161 ccgcagttct atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa
    47221 caggttgctg aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa
    47281 acggtacgct ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt
    47341 tacctgtata aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat
    47401 agcgaaaact tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg
    47461 ccgtattcag tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc
    47521 gaataattta tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattct
    47581 ccaggaaacg tgggaaag
//
LOCUS       DAZKIV010000019        42494 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZKIV010000019 DAZKIV010000000
VERSION     DAZKIV010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.019"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            137..472
                     /gene="eutS"
                     /locus_tag="ACHOT6_001113"
     CDS             137..472
                     /gene="eutS"
                     /locus_tag="ACHOT6_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFP9946368.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            485..964
                     /gene="eutP"
                     /locus_tag="ACHOT6_001114"
     CDS             485..964
                     /gene="eutP"
                     /locus_tag="ACHOT6_001114"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFP9946369.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            939..1640
                     /gene="eutQ"
                     /locus_tag="ACHOT6_001115"
     CDS             939..1640
                     /gene="eutQ"
                     /locus_tag="ACHOT6_001115"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFP9946370.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGQTMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            1637..2440
                     /gene="eutT"
                     /locus_tag="ACHOT6_001116"
     CDS             1637..2440
                     /gene="eutT"
                     /locus_tag="ACHOT6_001116"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFP9946371.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITHSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            2437..3453
                     /gene="pta"
                     /locus_tag="ACHOT6_001117"
     CDS             2437..3453
                     /gene="pta"
                     /locus_tag="ACHOT6_001117"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFP9946372.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQCLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTKVNRESSLQ
                     TLVE"
     gene            3492..3785
                     /gene="eutM"
                     /locus_tag="ACHOT6_001118"
     CDS             3492..3785
                     /gene="eutM"
                     /locus_tag="ACHOT6_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFP9946373.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            3892..4179
                     /gene="eutN"
                     /locus_tag="ACHOT6_001119"
     CDS             3892..4179
                     /gene="eutN"
                     /locus_tag="ACHOT6_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFP9946374.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            4191..5594
                     /locus_tag="ACHOT6_001120"
     CDS             4191..5594
                     /locus_tag="ACHOT6_001120"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFP9946375.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            5605..6441
                     /gene="eutJ"
                     /locus_tag="ACHOT6_001121"
     CDS             5605..6441
                     /gene="eutJ"
                     /locus_tag="ACHOT6_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFP9946376.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGLRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            6431..7618
                     /gene="eutG"
                     /locus_tag="ACHOT6_001122"
     CDS             6431..7618
                     /gene="eutG"
                     /locus_tag="ACHOT6_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFP9946377.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMVHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            7734..8960
                     /gene="eutH"
                     /locus_tag="ACHOT6_001123"
     CDS             7734..8960
                     /gene="eutH"
                     /locus_tag="ACHOT6_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFP9946378.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            8957..10360
                     /gene="eutA"
                     /locus_tag="ACHOT6_001124"
     CDS             8957..10360
                     /gene="eutA"
                     /locus_tag="ACHOT6_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFP9946379.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECLGAGTDVRSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            10372..11733
                     /gene="eutB"
                     /locus_tag="ACHOT6_001125"
     CDS             10372..11733
                     /gene="eutB"
                     /locus_tag="ACHOT6_001125"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFP9946380.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            11754..12641
                     /gene="eutC"
                     /locus_tag="ACHOT6_001126"
     CDS             11754..12641
                     /gene="eutC"
                     /locus_tag="ACHOT6_001126"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFP9946381.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLAELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            12651..13310
                     /gene="eutL"
                     /locus_tag="ACHOT6_001127"
     CDS             12651..13310
                     /gene="eutL"
                     /locus_tag="ACHOT6_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFP9946382.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            13323..13823
                     /gene="eutK"
                     /locus_tag="ACHOT6_001128"
     CDS             13323..13823
                     /gene="eutK"
                     /locus_tag="ACHOT6_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFP9946383.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDLGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            13869..14921
                     /gene="eutR"
                     /locus_tag="ACHOT6_001129"
     CDS             13869..14921
                     /gene="eutR"
                     /locus_tag="ACHOT6_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFP9946384.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(14927..15826)
                     /gene="hemF"
                     /locus_tag="ACHOT6_001130"
     CDS             complement(14927..15826)
                     /gene="hemF"
                     /locus_tag="ACHOT6_001130"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFP9946385.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(15830..16699)
                     /gene="amiA"
                     /locus_tag="ACHOT6_001131"
     CDS             complement(15830..16699)
                     /gene="amiA"
                     /locus_tag="ACHOT6_001131"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFP9946386.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            16913..17338
                     /locus_tag="ACHOT6_001132"
     CDS             16913..17338
                     /locus_tag="ACHOT6_001132"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFP9946387.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            17325..17774
                     /locus_tag="ACHOT6_001133"
     CDS             17325..17774
                     /locus_tag="ACHOT6_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFP9946388.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            17835..18410
                     /locus_tag="ACHOT6_001134"
     CDS             17835..18410
                     /locus_tag="ACHOT6_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFP9946389.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSKQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            18506..19405
                     /gene="yfeX"
                     /locus_tag="ACHOT6_001135"
     CDS             18506..19405
                     /gene="yfeX"
                     /locus_tag="ACHOT6_001135"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFP9946390.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(19463..20767)
                     /gene="pbp4b"
                     /locus_tag="ACHOT6_001136"
     CDS             complement(19463..20767)
                     /gene="pbp4b"
                     /locus_tag="ACHOT6_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFP9946391.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGVNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(20772..22196)
                     /gene="murP"
                     /locus_tag="ACHOT6_001137"
     CDS             complement(20772..22196)
                     /gene="murP"
                     /locus_tag="ACHOT6_001137"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFP9946392.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(22200..23096)
                     /gene="murQ"
                     /locus_tag="ACHOT6_001138"
     CDS             complement(22200..23096)
                     /gene="murQ"
                     /locus_tag="ACHOT6_001138"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFP9946393.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCWIINDEDKTVPLAVERV
                     LPDIAASIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23260..24117
                     /gene="murR"
                     /locus_tag="ACHOT6_001139"
     CDS             23260..24117
                     /gene="murR"
                     /locus_tag="ACHOT6_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFP9946394.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            24246..25037
                     /gene="ucpA"
                     /locus_tag="ACHOT6_001140"
     CDS             24246..25037
                     /gene="ucpA"
                     /locus_tag="ACHOT6_001140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFP9946395.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            25208..26224
                     /gene="cysP"
                     /locus_tag="ACHOT6_001141"
     CDS             25208..26224
                     /gene="cysP"
                     /locus_tag="ACHOT6_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFP9946396.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     LPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            26224..27057
                     /gene="cysT"
                     /locus_tag="ACHOT6_001142"
     CDS             26224..27057
                     /gene="cysT"
                     /locus_tag="ACHOT6_001142"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFP9946397.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            27057..27932
                     /gene="cysW"
                     /locus_tag="ACHOT6_001143"
     CDS             27057..27932
                     /gene="cysW"
                     /locus_tag="ACHOT6_001143"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFP9946398.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            27922..29019
                     /gene="cysA"
                     /locus_tag="ACHOT6_001144"
     CDS             27922..29019
                     /gene="cysA"
                     /locus_tag="ACHOT6_001144"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFP9946399.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHCWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            29153..30064
                     /gene="cysM"
                     /locus_tag="ACHOT6_001145"
     CDS             29153..30064
                     /gene="cysM"
                     /locus_tag="ACHOT6_001145"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFP9946400.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQQEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(30067..30435)
                     /locus_tag="ACHOT6_001146"
     CDS             complement(30067..30435)
                     /locus_tag="ACHOT6_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFP9946401.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            30540..31391
                     /gene="pdxK"
                     /locus_tag="ACHOT6_001147"
                     /gene_synonym="thiJ"
     CDS             30540..31391
                     /gene="pdxK"
                     /locus_tag="ACHOT6_001147"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFP9946402.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(31433..31942)
                     /gene="crr"
                     /locus_tag="ACHOT6_001148"
     CDS             complement(31433..31942)
                     /gene="crr"
                     /locus_tag="ACHOT6_001148"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFP9946403.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(31983..33710)
                     /gene="ptsI"
                     /locus_tag="ACHOT6_001149"
     CDS             complement(31983..33710)
                     /gene="ptsI"
                     /locus_tag="ACHOT6_001149"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFP9946404.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(33755..34012)
                     /gene="ptsH"
                     /locus_tag="ACHOT6_001150"
     CDS             complement(33755..34012)
                     /gene="ptsH"
                     /locus_tag="ACHOT6_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFP9946405.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(34396..35367)
                     /gene="cysK"
                     /locus_tag="ACHOT6_001151"
     CDS             complement(34396..35367)
                     /gene="cysK"
                     /locus_tag="ACHOT6_001151"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFP9946406.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(35552..36313)
                     /gene="cysZ"
                     /locus_tag="ACHOT6_001152"
     CDS             complement(35552..36313)
                     /gene="cysZ"
                     /locus_tag="ACHOT6_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFP9946407.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            36543..37529
                     /gene="zipA"
                     /locus_tag="ACHOT6_001153"
     CDS             36543..37529
                     /gene="zipA"
                     /locus_tag="ACHOT6_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFP9946408.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            37600..39615
                     /gene="ligA"
                     /locus_tag="ACHOT6_001154"
     CDS             37600..39615
                     /gene="ligA"
                     /locus_tag="ACHOT6_001154"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFP9946409.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            39617..39835
                     /locus_tag="ACHOT6_001155"
     CDS             39617..39835
                     /locus_tag="ACHOT6_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFP9946410.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(39832..40830)
                     /locus_tag="ACHOT6_001156"
     CDS             complement(39832..40830)
                     /locus_tag="ACHOT6_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFP9946411.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            40920..41846
                     /locus_tag="ACHOT6_001157"
     CDS             40920..41846
                     /locus_tag="ACHOT6_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9946412.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(41837..42178)
                     /locus_tag="ACHOT6_001158"
     CDS             complement(41837..42178)
                     /locus_tag="ACHOT6_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HFP9946413.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(42298..42373)
                     /locus_tag="ACHOT6_001159"
     tRNA            complement(42298..42373)
                     /locus_tag="ACHOT6_001159"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42338..42340),aa:Lys,seq:ttt)
     gene            complement(42378..42453)
                     /locus_tag="ACHOT6_001160"
     tRNA            complement(42378..42453)
                     /locus_tag="ACHOT6_001160"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42418..42420),aa:Val,seq:tac)
BASE COUNT         9879 a        10870 c        11955 g         9790 t
ORIGIN      
        1 aagtttatgc accacagcga atatctctcc attcttagtg atctacctca ccttttaaac
       61 gcgcttgccg aattttgtta tttactctga cgaaaaattg tcacgataca cgaaagtttt
      121 tcacaggcgg cgactcatgg ataaagaacg catcattcag gaatttgtgc ccggcaaaca
      181 ggtcacactg gcgcatctca tcgcgcatcc cggtgaggaa ctggcgaaga aaatcggcgt
      241 tccggatgcg ggcgcaattg gcattatgac gctaactccc ggcgaaacgg cgatgatcgc
      301 cggtgattta gcgctgaaag ccgctgatgt gcatatcggt tttctcgacc gcttcagcgg
      361 tgcattggtg atttatggct cggtgggcgc agtagaagaa gcgttatcac aaaccgtcag
      421 cggtttaggt cgcttattaa attatacgct ttgtgaaatg accaaaagtt aatacgaggc
      481 attcatgaaa cgaattgcgt ttgtgggtag cgtcggggca ggaaaaacaa cgttatttaa
      541 tgcgttgcag gggaattata ccctcgccag aaaaacacag gccgtggaat ttaatgataa
      601 gggcgatatt gatacgccgg gtgaatattt tagccatccc cgctggtatc acgccttaat
      661 taccacgcta caggatgtag acatgctgat ctatgtccat ggcgcaaatg acccggaaag
      721 tcggttgcct gccgggttgc tggatattgg cgtcagcaaa cggcaaatcg ccgtcatcag
      781 taaaacggac atgccagatg ctgacgtcgc cgcaacgcga aagttattgc ttgagacggg
      841 tttcgaagag ccgattttcg agctaaacag ccacgacccg caaagcgtgc agcaactggt
      901 ggattatctg gcatctctca ccaaacagga ggaagcaggt gaaaaaactc atcacagcga
      961 atgatattcg tgaagcacac gcgcgcggcg aacaggcaat gtccgtggtt ctgcgcgcca
     1021 gcattattac cccggaagcc cgtgaagtgg cggatctact gggttttacc atcaccgaat
     1081 gtgatgaatc gatcccggta acggcgtccg tgcccgccag cgtgccagcc gacaaaaccg
     1141 aaagccagcg catccgcgaa accatcattg cccagctgcc ggaaggccag tttactgaaa
     1201 gcctggtcgc acagctgatg gaaaaagtga tgaaggaaaa acagtcgctg gagcagggcg
     1261 caatgcagcc gagcttcaaa tcggtgaccg gcaaaggcgg catcaaagtg attgatggca
     1321 gcagcgtcaa gtttggtcgt ttcgacggcg cagagccgca ttgcgtgggc ttgactgatc
     1381 ttgtgaccgg agacgacggc agcagcatgg ccgccggttt tatgcagtgg gagaacgcat
     1441 tcttcccgtg gaccctgaac tacgacgaaa tcgacatggt gctggaaggg gagctgcatg
     1501 ttcgccacga aggccaaacc atgatcgcca aagcaggcga cgtgatgttt atcccgaaag
     1561 gctccagcat cgaatttggt acgacatcca gcgtgaaatt cctgtatgtc gcctggccgg
     1621 ctaactggca atccctatga aagatttcat caccgaagca tggctaagag cgaaccatac
     1681 gctcagcgaa ggcgcagaga tccatctccc tgcggacagt cgcctgacgc cctctgcccg
     1741 ggagttgctg gaaagccgcc atctgcgcat caagtttatt gacgagcagg gccgcctgtt
     1801 tgttgacgat gaacagcagc agccgcagcc cgttcatggg ctgaccagta gcgatgaaca
     1861 tccgcaggcg tgctgcgaac tgtgccgcca gccggtggca aaaaagccgg atacgctgac
     1921 tcacctgtcg gcggaaaaaa tggtcgccaa aagcgatccg cgtctgggtt ttcgcgcggt
     1981 tctagacagc accattgcgc tggcagtgtg gctgcaaatt gaactggcgg aaccgtggca
     2041 gccgtggctg gcggatatcc gttcgcgtct tggcaacatt atgcgcgccg atgcgctggg
     2101 ggaaccgctg ggctgccagg cgattgtcgg gctttctgac gaagatctgc atcggctttc
     2161 tcatcagccg ctgcgctatc tcgaccacga tcatctggtg ccggaagcca gccacgggcg
     2221 cgatgctgcg ttgctcaatc tgctacgcac caaagtgcgg gaaaccgaaa ccgtagcggc
     2281 gcaggtcttt atcacccaca gctttgaagt attacgcccg gacattttgc aagcgctgaa
     2341 ccgcctttcc agcacggtct acgtgatgat gatcctgagc gtaacgaagc agccgctaac
     2401 ggtgaagcag atccaacaac gactggggga aacgcaatga ttattgaacg ttgtcgtgaa
     2461 ctggcgttgc gagcgcccgc cagagtagtt tttccggatg cgttagatca acgtgtgctg
     2521 aaagctgcgc aatatttaca tcaacaatgt ctggcaacgc ccattctggt cgccaatccg
     2581 tttgaacttc gtcagtttgc gctcagtcac ggcgtggcga tggacgggct acaggtgatt
     2641 gatccgcatg gcaacctcgc aatgcgggaa gaatttgctc atcgctggct ggcccgcgcg
     2701 ggcgaaaaaa cgccgccgga tgcgctggaa aaacttaccg atccgctgat gttcgccgca
     2761 gcaatggtca gcgccggtaa agcggatgtc tgtatcgcgg gcaacctctc ttccacggcg
     2821 aatgtgctgc gtgccggatt acgcattatc ggcttgcagc caggctgtaa aacgctctca
     2881 tccattttcc tgatgttgcc acagtacagc ggcccggcgt tgggctttgc cgattgcagc
     2941 gtggtgccac agccgacggc ggcgcaactg gcggatatcg cgcttgccag tgccgaaacc
     3001 tggcgcgcca tcaccggaga agagccgcgc gtggcgatgc tgtcgttttc cagtaacggt
     3061 agcgcccgtc acccctgcgt tgccaacgtc cagcaggcga cagaaatcgt ccgtgagcgc
     3121 gcaccaaagc tggtggttga tggcgagtta cagtttgacg ccgccttcgt gccggaagtg
     3181 gcggcgcaaa aagcgcctgc cagcccgcta cagggcaagg ccaatgtgat ggtttttccg
     3241 tcgctggaag ccggaaatat tggttacaaa atcgcacaac gactcggcgg atatcgtgcc
     3301 gtcgggccac tgatacaagg acttgccgcg ccgatgcacg atctctctcg tggttgtagc
     3361 gtgcaggaaa ttatcgagct ggcgctggtg gcagctgtgc cgcgtcagac aaaagtgaac
     3421 cgcgaaagca gtttacaaac actggttgaa tgaacggtcc cgttctggac cctgataaag
     3481 aggaaagcac aatggaagca ttaggaatga tcgaaacccg gggcctggtt gcactgattg
     3541 aggcttctga cgcgatggtg aaagctgcgc gcgtcaagct ggttggtgtt aagcagattg
     3601 gcggcggcct gtgtactgcc atggttcgcg gtgatgttgc agcctgtaaa gcggcgaccg
     3661 atgccggtgc cgcagcggca cagcgtattg gcgagctggt atccgtacac gttatcccgc
     3721 gtccgcacgg tgacctggaa gaagtcttcc cgatcggcct gaaaggcgat agcagcaacc
     3781 tgtaatcacc ctgtgtgacg tcgattcggg cgtcacgctc tctccttttt cattttacct
     3841 tctgcggagg gtagggggag cttttacctg aaatacgcca cggaggcggg tatgaaactg
     3901 gcagtcgtca ctggacaaat tgtttgtacc gtacgccatc acggactggc gcatgacaaa
     3961 ttgctgatgg tggaaatgat tgatccacaa ggtaatcctg acgggcaatg cgccgtcgcc
     4021 atcgacaata ttggcgcggg aaccggggag tgggtgttac tggtgagtgg cagttccgcc
     4081 cgccaggcgc ataaaagcga aacgtcaccg gtcgatctgt gcgtgattgg cattgtcgat
     4141 gaggtggtgt ctggcggtca ggtaattttc cacaaataag gcagaacatc atgaatcaac
     4201 aggatattga acaggtggtg aaagcggtac tgctgaaaat gcaaagcagt gacacgccgt
     4261 ccgccgccgt tcatgagatg ggcgttttcg cgtccctgga tgacgccgtt gcggcagcca
     4321 aagtcgccca gcaagggtta aaaagcgtgg caatgcgcca gttagccatt gctgccattc
     4381 gtgaggcagg cgaaaaacac gccagagatt tagcggaact tgccgtcagt gaaaccggca
     4441 tgggccgcgt tgaagataaa tttgccaaaa acgtcgctca ggcgcgcggc acgccaggcg
     4501 ttgagtgtct ctctccgcag gtgctgaccg gcgacaacgg cctgacttta attgaaaacg
     4561 cgccctgggg cgtggtggct tcggtaactc cgtccactaa cccggcggca accgtaatta
     4621 ataacgccat cagcctgatt gccgcgggca acagcgtcat ttttgccccg catccggcgg
     4681 cgaaaaaagt ctcccagcgg gcgattacgc tgctcaacca ggcgattgtt gccgcaggtg
     4741 ggccggaaaa cttactggtt actgtggcaa atccggatat cgaaaccgcg caacgcttgt
     4801 tcaagtttcc gggtatcggc ctgctggtgg taaccggcgg cgaagcggta gtagaagcgg
     4861 cgcgtaaaca caccaataaa cgtctgattg ccgcaggcgc tggcaacccg ccggtagtgg
     4921 tggatgaaac cgctgacctc gcccgtgctg ctcagtccat cgtcaaaggc gcgtctttcg
     4981 ataacaacat catttgtgcc gacgaaaagg tactgattgt tgttgatagc gtagccgatg
     5041 aactgatgcg tctgatggaa ggccagcacg cggtgaaact gaccgcagaa caggcgcagc
     5101 agctgcaacc ggtgttgctg aaaaatatcg acgagcgcgg aaaaggcacc gtcagccgtg
     5161 actgggttgg tcgcgatgcg gcgaaaatcg cggcggcaat cggcctgaac gtgccgcaag
     5221 aaacgcgttt gctgtttgtg gaaaccactg cagaacatcc gtttgccgtg accgaactga
     5281 tgatgccggt gctgcccgtc gtgcgcgtcg ccaacgtggc ggatgccatt gcgctggcgg
     5341 tgaaactgga gggcggttgc caccacacgg cggcaatgca ctcgcgcaat atcgaaaaca
     5401 tgaaccagat ggcgaacgcc attgatacca gcattttcgt taagaacgga ccgtgcattg
     5461 ccgggctggg gctgggcggg gaaggctgga ccaccatgac catcaccacg ccaaccggtg
     5521 aaggggtaac cagcgcgcgg acgtttgtcc gtctgcgtcg ctgtgtatta gtcgatgcgt
     5581 ttcgcattgt ttaaggagta accgatggcg cacgacgaac aatggctcac cccacgtctg
     5641 caaacggcgg caacgctgtg taaccagacg cctgccgcga cggaatctcc gctgtggctg
     5701 ggggtggatc tgggcacctg cgatgtggtg tcgatggttg ttgaccgcga cggtcagccg
     5761 gtggcggtat gcctcgactg ggccgatgtg gtccgcgacg gcatcgtctg ggatttcttc
     5821 ggtgcggtca ccattgttcg tcgccatctc gacactcttg agcagcaatt cggtcttcgt
     5881 tttagccatg cggcgacttc atttcctcca ggtacggacc cgcgtatttc catcaacgtg
     5941 ctggaatctg ccgggctgga agtgagccat gtgctggatg agccaaccgc agtcgcggat
     6001 ctgctgcaac tggacaacgc cggtgtagtg gatatcggcg gcggcactac cggcattgcc
     6061 atcgtgaaaa agggcaaggt gacgtactcg gcggatgaag caaccggcgg acatcacatc
     6121 tctctgactc tcgccggaaa tcgccgtatt tcgctggaag aggcggagca gtacaagcgc
     6181 ggtcacggtg aagagatttg gcctacggtg aaaccggtgt acgaaaaaat ggcggacatt
     6241 gtcgcccggc atattgaagg gcagggcatt actgatttat ggctggcggg cggctcttgt
     6301 atgcaaccgg gcgtggcgga actgtttcgc aaacaattcc cggcgttaca ggtgcattta
     6361 ccgcagcaca gtttgtttat gaccccgctg gcgatcgcca gtagtgggcg agagaaagcg
     6421 gaggggctat atgcaaaatg aattgcagac cgcgctcttt caggcgttcg ataccctgaa
     6481 tctgcaacgg gtaaaaacat ttagcgttcc accggtgacg ctttgcggtc cgggctcggt
     6541 gagcagttgc ggacagcaag cgcaaacgcg tgggctgaaa catctgttcg tgatggcaga
     6601 cagctttttg catcaggcag ggatgaccgc cgggctgacg cgcagcctgg ctgttaaagg
     6661 catcgccatg acgctctggc catgtccggt gggcgaaccg tgcatcaccg acgtgtgtgc
     6721 agccgtggcg cagttgcgtg agtcaggctg tgatggggtg atcgcatttg gcggcggctc
     6781 ggtgctggat gcggcgaaag ccgtggcgtt gctggtgacg aacccggata gcacgctggc
     6841 agagatgtca gaaaccagcg ttctgcaacc gcgcttgccg ctgattgcca ttccaacgac
     6901 cgccggaacc ggctctgaaa caaccaatgt aacggtgatt atcgacgcgg tgagcgggcg
     6961 caagcaggtg ttagcccatg cctcgctgat gccggatgtg gcgatcctcg acgccgcatt
     7021 gaccgaaggt gtgccgtcgc atgtcacggc gatgaccggc attgatgcgt taacccatgc
     7081 cattgaagca tacagcgccc tgaacgctac accgtttacc gacagcctgg cgattggcgc
     7141 tattgcgatg attggcaaat cgctgccgaa agcggtgggc tacggtcacg accttgccgc
     7201 gcgcgagagc atgttgctgg cttcatgtat ggcgggaatg gcgttttcca gtgcgggtct
     7261 tgggttgtgc cacgcgatgg tgcatcagcc gggcgcggcg ctgcatattc cacacggtct
     7321 ggcgaacgcc atgttgctgc caacggtgat ggaatttaac cggatggttt gtcgtgaacg
     7381 ctttagtcag attggtcggg cactgcgaac taaaaaatct gacgatcgtg acgctattaa
     7441 cgcggtaagt gagctgattg cggaagttgg gattggtaaa cgactgggcg atgttggtgc
     7501 gacatctgcg cattacggcg catgggcgca ggccgcgctg gaagatattt gtctgcgcag
     7561 taacccgcgt accgccagcc tggagcagat tgtcggcctg tacgcagcgg cgcaataaat
     7621 gccggatgcg acgcttgccg cgtcttatcc ggcctacggg attgcacatg tagggcggat
     7681 aaggcttcac gcagcatccg ctaataaata acaactaaca gggagtaaag gcgatgggaa
     7741 ttaacgaaat catcatgtac atcatgatgt tctttatgct gatagctgcc gtagacagga
     7801 tcctgtcgca gttcggcggt tctgctcgtt tcctcggtaa gttcggtaaa agtatcgaag
     7861 gatcaggcgg tcagttcgaa gaaggcttta tggcaatggg cgctctgggt ctggcgatgg
     7921 tcggtatgac cgcgctggca ccggtactgg ctcacgtact cgggccggta attatcccgg
     7981 tttacgaaat gctcggcgca aacccgtcaa tgttcgccgg aacgctgctg gcgtgcgata
     8041 tgggcggctt cttcctcgcc aaagagctgg cgggcggcga cgtagcagcg tggctatact
     8101 ctgggttaat tcttgggtcg atgatggggc caacgattgt gttttccatt ccggtggcgc
     8161 tcggcattat cgaaccttct gaccgtcgtt atctggcgct cggcgtgctg gcgggcattg
     8221 tgaccattcc gattggctgt attgctggtg gtctggttgc tatgtactcc ggtgtgcaga
     8281 tcaacggtca gccggtggaa ttcactttcg ccctgatcct gatgaacatg atcccggtga
     8341 tcattgttgc gattctggtg gcgctggggc tgaaattcat cccggaaaaa atgatcaacg
     8401 gcttccagat cttcgccaaa ttcctcgttg cattgatcac cctcggtctt gccgctgcgg
     8461 tagtgaaatt cctgcttggc tgggaactga tccccggtct ggatcctatc tttatggccc
     8521 ctggcgataa acccggtgag gtgatgcgcg ccattgaagt tatcggttct atctcctgcg
     8581 ttctgttagg ggcgtatccg atggtgctgc tgctgacccg ctggtttgaa aaaccgctga
     8641 tgagcgtcgg taaagtgctg aatatgaaca acatcgcggc agccggcatg gtggcaacgc
     8701 ttgccaacaa catcccgatg ttcggcatga tgaagcagat ggatacccgc ggcaaagtca
     8761 tcaactgcgc cttcgccgtt tccgctgctt tcgccctggg cgaccactta ggcttcgccg
     8821 ctgccaacat gaatgccatg atcttcccga tgattgtcgg caagttgatc ggcggcgtaa
     8881 cggcgattgg cgtggcgatg atgctggtgc caaaagaaga cgcgaccgcg actaaaaccg
     8941 aagcggaggc acaatcgtga acactcgcca gctattgagc gtcggtatcg atatcggcac
     9001 caccaccacc caggtgattt tctcccacct ggaactggtt aaccgtgcgg cggtgtcgca
     9061 ggtgccgcgc tacgaattca ttaaacgcga aattagctgg caaagcccgg tgttctttac
     9121 ccctgtcgat aaacagggtg gtttaaaaga agcggaactg aaatcgctga ttttagagca
     9181 atatcaggct gcgggcattg cgccggaaag cgttgattct ggtgccatca tcatcaccgg
     9241 tgaaagcgcg aaaacccgca atgctcgccc ggcggtgatg gcgctctctc agtcgctggg
     9301 cgattttgtc gtcgccagcg ccgggccgca cctcgaatcc gtgatcgccg gtcacggtgc
     9361 cggggcgcaa accctttctg aacaacggct gtgtcgggta ctgaatatcg acatcggcgg
     9421 tggcaccgcg aactacgccc tgttcgatgc cggaaaaatc agcggcactg cctgcctcaa
     9481 cgtcggtggt cgcctgctgg aaaccgacag ccaggggcgc gtggtttacg ctcataaacc
     9541 ggggcagatg attgtggatg agtgtttagg tgcaggcact gacgtccgtt cgctgaccgg
     9601 cgcgcagctg gtgcaggtta cccggcggat ggcggcgctg attgtcgaag tgattgacgg
     9661 aacgctttcg ccgctcgcgc aggcattgat gcaaaccggt ttgctgcccg caggtgttac
     9721 gcccgaaatc attacgcttt ctggcggcgt gggcgaatgt tatcgccacc agcccgccga
     9781 cccgttctgt tttgccgata ttggcccact tctggcaacg gcgctgcatg accatccgcg
     9841 cctgcgtgag atgaatgtgc agtttccggc gcaaaccgta cgcgccacgg tgattggcgc
     9901 gggtgcacat accctttcgc tctctggcag cacaatctgg ctggagggcg tacaactgcc
     9961 gctgcgcaat ttgccggtgg cgatcccgat tgatgaaacg gatctggtga gtgcctggca
    10021 acaggcgctg cttcagctgg atcttgatcc caaaactgac gcgtacgtgc tggcgcttcc
    10081 cgcctcgctg cctgtgcgtt acgccgcggt actgacggtc atcaacgcgc tggtcgattt
    10141 cgtcgcgcgt tttccgaatc cgcatcccct gctggtggtg gccgggcagg actttggtaa
    10201 agctctgggc atgttgttgc gcccacagct acaacaactc ccgttggcag tcattgacga
    10261 agtgattgtc cgcgcggggg actatatcga cattggtacg cctctttttg gcggatcggt
    10321 tgtgccggtg acggtgaaat cactcgcatt tccttcctga gggaacgact tatgaaacta
    10381 aagaccacat tgttcggcaa tgtatatcag tttaaggatg taaaagaggt gctggctaaa
    10441 gccaacgaac tgcgttcggg ggatgtgctg gcgggcgttg cagcggcaag ctcacaggag
    10501 cgcgtggcgg caaagcaggt gttgtcggaa atgaccgtag cggacatccg caataatccg
    10561 gtgattgcct atgaagatga ctgcgtgacg cggctgattc aggacgatgt taacgaaacg
    10621 gcctacaacc agattaaaaa ctggagcatc agcgaactgc gtgagtatgt gctgagcgat
    10681 gaaaccagcg tggacgacat tgcctttacc cgcaaagggc tgacctcgga agtggtcgcg
    10741 gcggtagcga agatttgctc caacgcggac ctgatctacg gcgcgaagaa aatgccggta
    10801 atcaaaaagg ccaataccac cattggtatt ccgggcacct tcagcgcccg tttgcagcca
    10861 aatgacaccc gtgacgacgt gcaaagtatc gccgcgcaaa tctacgaagg gctttccttc
    10921 ggggtgggcg atgcggtgat cggcgttaac ccggtgactg acgacgtgga aaacttaagc
    10981 cgcgtgttgg acaccattta cggcgtgatc gacaaattca acatcccaac tcagggctgc
    11041 gtactggcgc acgtcaccac ccagatcgaa gcgattcgtc gcggcgcgcc tggcggactg
    11101 attttccaga gtatctgtgg cagcgaaaaa gggctgaaag agtttggcgt ggaactggcg
    11161 atgctcgacg aagcgcgcgc agtgggtgca gagttcaacc gtatcgccgg ggaaaactgc
    11221 ctctacttcg aaaccggaca aggctctgcg ctatccgctg gcgctaactt cggcgctgac
    11281 caggtgacga tggaagcacg taactatggg ctggcgcgtc attacgatcc gtttatcgtc
    11341 aacaccgtgg tcggctttat tgggccggag tatctctaca acgaccgcca gattatccgt
    11401 gctggcttag aagatcactt tatgggcaag ctgagcggca tctctatggg ctgtgactgc
    11461 tgttacacca accacgctga cgctgaccag aacctcaacg aaaacctgat gatcctgctc
    11521 gccaccgcag gctgcaacta catcatgggg atgccgctgg gtgatgacat catgctcaac
    11581 taccagacca ccgcattcca cgataccgcc actgtgcgtc agttactcaa cctgcgcccg
    11641 tcaccggagt ttgaacgctg gctggaaagc atgggcatta tggcaaacgg tcgcctgacc
    11701 aaacgggcgg gcgatccgtc actgttcttc tgatgacgcg gagataacac atcatggatc
    11761 aaaaacagat tgaagaaatt gtacgcagcg tgatggcgtc aatgggacaa gcggcccccg
    11821 cgccgtcaga agcaaagtgc gccaccacca actgtgcggc accggtgacc tcggaaagct
    11881 gcgcgctgga tttaggctct gctgaagcaa aagcgtggat tggtgttgaa aacccgcatc
    11941 gcgcagacgt attagcagaa ctgcgccgca gcaccgtggc ccgcgtttgt accggtcgtg
    12001 ccggtccgcg tccacgtacg caggcgctgc tgcgtttcct ggccgatcac tctcgttcga
    12061 aagataccgt actgaaagaa gtgccggaag agtgggtgaa agcgcagggc ttgctggaag
    12121 tacgctccga aattagcgac aaaaatctct acctgactcg cccggatatg ggccgccgcc
    12181 tgtgtgcaga agctgttgaa gcgctgaaag cacagtgtgt tgctaatccg gacgtacagg
    12241 tcgttatttc tgatggcttg tcaactgatg ccatcactgt gaactacgaa gagatcctgc
    12301 cgccgctgat ggcgggcctg aaacaggccg ggctgaaagt cggcacgcca ttctttgttc
    12361 gttatggtcg cgtgaagatt gaagatcaga tcggtgagat cctcggcgcg aaagtggtga
    12421 tcctgctggt gggggaacgt ccggggttgg gtcagtcaga aagcctctcc tgctacgccg
    12481 tttactcgcc gcgtatggcg accaccgttg aggctgatcg cacctgcatc tctaacattc
    12541 accagggcgg cacgccgccg gttgaagccg cagcggtaat tgtggatttg gcgaaacgta
    12601 tgctggagca gaaagcatcc ggcatcaaca tgacccgata aggaggcatc atgccagctt
    12661 tagatttgat tcgaccgtcg gtaaccgcca tgcgggtgat tgcctccgtt aacgctgaat
    12721 ttgcgcgtga actaaaattg ccgccgcata ttcgtagcct cgggctgatt tctgccgact
    12781 ccgatgacgt cacctacatt gcggctgatg aagcgaccaa acaggcgatg gtggaagtgg
    12841 tgtatggccg ctcgctgtat gcgggcgctg cacacggccc gtcaccgact gccggtgagg
    12901 tgctgattat gctgggtggg ccaaacccgg cggaagtgcg tgctggtctg gatgcgatgg
    12961 ttgcacatat tgaaaatggc gctgctttcc agtgggctaa tgacgcagaa aacaccgcat
    13021 tcctggcaca tgtagtttcg cgtaccggtt cttatctctc atcaaccgcc gggatcacgc
    13081 ttggcgatcc gatggcgtat ctggtggcac cgccgctgga agcgacctac ggcattgatg
    13141 cagcgttgaa atctgccgac gtacagctgg tgacctatgt cccgccaccg tctgaaacca
    13201 actactcggc agcattttta accggtagcc aggccgcgtg taaagcagcc tgtaacgcct
    13261 ttaccgatgc agtgctggaa atcgcgcgta atccaatcca gcgtgcgtaa cggaggttgc
    13321 cgatgatcaa tgcactggga ttgctggaag tggacggcat ggtcgccgcg atagatgcag
    13381 cggatgccat gctcaaagca gctaacgttc gtctgctcag tcacgaagtg cttgaccttg
    13441 gtcgcttaac gctggtggtg gaaggcgatc tggcggcgtg tcgtgcggcg ctggacgcag
    13501 gttgtgctgc cgcgatgcgt accgggcgtg tcatcagccg caaggagatc ggtcggccag
    13561 acgatgacac ccagtggctg gttactggct ttaaccgcca gccgaagcaa cccgtaaggg
    13621 aacccgacgc gccagttatc gtcgcggaat ctgctgacga gttgttggcg ctgttaacat
    13681 cagtacgtca gggaatgacg gcaggagaag tggctgccca ctttggctgg ccgctggaaa
    13741 aagccagaaa tgcgctcgaa cagctctttt ctgccgggac gttacgtaaa cgcagtagtc
    13801 gttatcgtct caagccccat taacctgtcg gaggtgccgg gtgtcctaca acacccggca
    13861 ttaacatcat gaaaaagacc cgtacagcca atttgcacca tctttatcat gaacccttac
    13921 ccgaaaacct gaagctcacg ccgaaggtcg aagtggataa tgttcatcaa cgacagacaa
    13981 cggatgtcta tgaacatgct ttaacgatta ccgcctggca gcagatttac gatcagctgc
    14041 atccgggcaa gtttcatggt gaatttacgg aaattctact cgatgatatt caggtttttc
    14101 gtgaatacac cggtctggcg ctgcgtcagt cgtgcctggt ctggccgaac tcgttctggt
    14161 ttggcattcc ggcgacgcgc ggtgagcagg gatttatcgg ttcgcaatgt ctgggaagcg
    14221 cggaaatcgc cacccgccct ggtggcactg aatttgaact gagcacgccg gatgattaca
    14281 cgatcctggg cgtggtgctt tctgaagatg tcatcacccg gcaggctaac tttttgcata
    14341 acccggatcg ggtattacat atgctgcgta gccagtcggc gctggaagtg aaagagcagc
    14401 ataaagccgc gctgtggggc tttgtccaac aggcgctggc gacgttttgc gagaacccgg
    14461 aaaatctcca tcagcctgca gtgcgaaaag tgctggggga taatttgcta atggcgatgg
    14521 gggcgatgct ggaagacgcg caaccaatgg tgacggcgga aagcatcagt catcagagtt
    14581 accgtagatt actttcccgc gcccgtgaat atgtgctgga aaatatgtct gagccggtga
    14641 cggtgctgga cttgtgtaat caactgcatg tcagtcgccg cacgctacaa aacgcgtttc
    14701 acgctatttt aggcattggc ccaaacgcgt ggctgaaacg cattcgcctg aacgccgtac
    14761 gccgcgaact gataagcccg tggtcgcaaa gcacaacggt aaaagacgcc gccatgcagt
    14821 ggggattctg gcatctgggg caatttgcca cggattacca gcagctgttt gccgagaagc
    14881 cgtcgttgac gttgcatcag cggatgcggg aatgggggtg agggagttac acccaatccc
    14941 tgaccttaat aaactcactt aacgccgctt ctgggctgcc atcttttggc tgataatcat
    15001 actcccagcg taccagtggc ggcattgaca tcaggatcga ctcggtgcgc ccgccagttt
    15061 gcaggccaaa cagagtgccg cgatcccaga ccagattgaa ctcgacataa cgaccgcgac
    15121 ggtagagctg aaaattgcgc tcgcgctcgc cgtaggccat cgctttacgt cgctctacaa
    15181 ttggtaaata agcgtcggtg tagcctttgc ctaccgcctg cataaaggca aaacagtggt
    15241 cgaaatctgg cgtgttcaga tcatcaaaga acagcccgcc aataccgcgc tgttcgttgc
    15301 gatgtttgag gtagaagtaa tcgtcgcacc actttttgta acggggataa acgtcttcac
    15361 caaatggcag gcacaggtca cgggcggtgc ggtgccagtg aatggcgtct tcttcaaaac
    15421 catagaaagg ggttaaatcg aagccgccgc caaaccacca gacgggctct gcacccggtt
    15481 tttcggcaat aaaaaaacgc acattcgcgt ggctggtggg aacatacggg ttatgcggat
    15541 gcactaccag tgaaacgccc atcgcctcga aactgcgccc ggcaagttcc gggcgatgag
    15601 cggtggcgga agctggcatc gcctcgccgt ggacgtgaga aaagttgacg cctgcctgtt
    15661 cgaaaacacc accattacgc aacacccgac tacgcccgcc gccgccagct tcgcgctgcc
    15721 aactatcttc gacaaattct gcgccatcga cggcggtcag ctgctgacaa atcgtatcct
    15781 gaaggttgag cagaaactgt ttaacctggt gtgcgtcggg tttcataact tatcgctttt
    15841 tcgaatgtgc tttctggttg tcgaaccagt ggaaataact gatcacgcct tcagcaatcg
    15901 ctgtggcgat tttctgacga aacgccgccg tgcctaacag ccgctcttct tccgggttgg
    15961 tgataaacga ggtttccacc agcaccgaag gaaccgacgg cgatttcaac accacaaacg
    16021 cggcttgttc ggtgttgcgg ctgtgcagtt tatgcaccgg cttaatcttt ttcagaatat
    16081 gcgagccgag cgtcaggctg tttttaatag tatcggtttg caccagatca aacagcactt
    16141 gctgcaatag gtgatccttg tcagtcgcct ttttaccggc aacttcatcg gcgcggtttt
    16201 cgcgttcaga caggtatttc gccattgcgc tacttgcccc acggttagag agggcaaata
    16261 ccgaagcacc ggcagctttc gggttggtaa agccatcggc atgaattgac ataaacagat
    16321 ctgcgccatg tttatgggcg atttcaacgc gatcgtaaag tgggataaac gtatcgccag
    16381 aacgcgttaa acgcgcatca atcccatgat tacgcaaaat ggaacggacg tttttagcaa
    16441 tcgccagcac cacatgtttt tctttcgaac cgttgcgtcc gatcgctccg gtatcaattc
    16501 cgccgtggcc tggatcgaga acaacgacac gtttgccgcc agattttttg gctttcggct
    16561 tgctgtgtcc gttgctggtt tttaaaggtt cgtctttggc gatggcttgc gacattcctg
    16621 acaacgtcag ggcagccaaa ccggctttca gcacctggcg gcgcgaagtg agtgttttta
    16681 gtggtttaaa agtgctcata cggcctgagt tgtaataata aagttccaga tgttatatcg
    16741 tatcgcgtaa tccgttacga cggtttgatg ttgagaattg ttttactttt catttcaata
    16801 gttgacaact ccccattatg ccactttttc aaccgatttt cgtcagatat tggctaattc
    16861 gaccgttcac gccataatca gccttttaac ccgcaaaaag gtaacgaata ccatggagat
    16921 acgcgtattt cgccaggaag atttcgaaga ggtcatcacc ctttgggagc gttgcgactt
    16981 gctgcgtccg tggaacgatc cggaaatgga catcgagcgt aagatgaacc atgacgtcag
    17041 tttgtttttg gtcgctgagg taaacggtga agtggtcgga acggtgatgg gcggttatga
    17101 cgggcatcgc gggtctgctt attatcttgg cgtgcatcca gagtttcgtg ggcgtgggat
    17161 tgccaatgcg ttgcttaatc ggctggagaa aaagctgatt gctcgtggct gcccgaaaat
    17221 tcagatcaat gtgccggaag ataacgacat ggtgctcgga atgtatgaac gcctgggata
    17281 tgaacacgcc gacgtgctga gtctgggtaa gcgtttgatt gaagatgaag agtactgagt
    17341 tccatcctgt ccattatgat gcgcacggac gcctgcgttt acccttgctc ttctggcttg
    17401 tgctattgct tcaggcgcga acctgggtgc tgtttgtcat tgccggtgcg tcgcgcgagc
    17461 agggtactgc gctgctaaat ctgttttatc ccgatcacga taatttctgg ctggggctga
    17521 ttccaggcat tccggcggtg ctggcgtttt tgttgagcgg tcggcgggct acatttcctc
    17581 gcacttggca tgtgctctat tttctgttgc tgttggcgca ggtggtttta ctttgctggc
    17641 aaccgtggct gtggctgaac ggtgaatccg ttagcggtat cggtctggcg ctggtagtgg
    17701 cggatattgt ggcgttaatc tggctgctga ccaatcgacg tttacgcgct tgttttaatg
    17761 aagaaaaaga ataacggcac tttttggtga atttgcactc caagcaacgt tattgaataa
    17821 ccaaaggaag tgacatgaaa tcgctgcgtt taatgttatg cgctatgccg ttgatgctga
    17881 ccggctgttc cacgatgtcg tcagttaact ggtcggccgc taacccgtgg aactggtttg
    17941 gatcgtccac caaagtgagc aagcagggcg tgggtgaatt aacggcgtcc acaccactgc
    18001 aagaacaagc cattgctgat gcgcttgatg gcgattatcg cctgcgcagc ggaatgaaaa
    18061 ccgcgaacgg caacgtggtg cgcttttttg aagtgatgaa aggcgacaac gtggcgatgg
    18121 tgattaacgg cgatcaaggc acgatcagtc gcattgacgt gctggatagc gatattcctg
    18181 ctgacaccgg tgttaaaatc ggtacaccgt ttagcgacct ttacagcaaa gcatttggca
    18241 attgccaaaa agctgatggt gatgataatc gtgccgtcga atgtaaagcc gaaggcagtc
    18301 aacatattag ctaccagttc agcggggaat ggcgtggtcc tgaagggtta atgccttcgg
    18361 acgataccct gaaaaactgg aaagtcagta aaattatctg gcggcgttaa tttgcgtctg
    18421 aacaaaccgc cgccgcgaaa aaacacgtaa aatagcgctc aacaatgcca cggattgcgt
    18481 ggcattctta ttttcaggag gaacaatgtc tcaggttcag agtggcattt tgccagaaca
    18541 ttgccgcgcg gcgatttgga tcgaagccaa cgtgaaaggg gaagttgacg ccctgcgtgc
    18601 ggccagtaaa acatttgccg acaaactggc aacttttgaa gcgaaattcc cggacgcgca
    18661 tcttggtgcg gtggttgcct ttggtaacaa cacctggcgc gctctgagcg gcggcgttgg
    18721 ggcagaagag ctgaaagatt ttccgggcta cggtaaaggc cttgcgccga ccacccagtt
    18781 cgatgtgttg atccacattc tttctctgcg tcacgacgta aacttctctg tcgcccaggc
    18841 ggcgatggaa gcctttggtg actgcattga agtgaaagaa gagatccacg gcttccgttg
    18901 ggtggaagag cgtgacctga gcggctttgt tgacggtaca gaaaacccgg cgggtgaaga
    18961 gacgcgccgc gaagtggcgg ttatcaaaga cggcgtggat gccggcggca gctatgtgtt
    19021 tgtgcagcgt tgggagcaca acctgaagca gctcaaccgg atgagcgttc acgatcagga
    19081 gatgatgatc gggcgcacca aagaggccaa cgaagagatt gacggcgacg aacgtccgga
    19141 aacctctcac ctcacccgcg ttgatctgaa agaagatggc aaagggctga agattgttcg
    19201 ccagagcctg ccgtacggca ccgccagtgg cactcacggt ctgtacttct gcgcctactg
    19261 cgcgcgtctg cataacattg agcagcaact gctgagcatg tttggcgata ccgatggtaa
    19321 gcgtgatgcg atgttgcgtt tcaccaaacc ggtaaccggc ggctattact tcgcgccgtc
    19381 gctggacaag ctgatggcgc tgtaagcgtt tctgattcta tgattaaagg ccagccagta
    19441 acaaaactga ctggcctttt tattactttt gcttcaacgc cgcatacacc tgatcaacta
    19501 cccaaccata agttgcaatc ggtaactgac cgctttcgaa catattggga tttttttgtg
    19561 gatcggcaac tggcgaatgg ggcttgttgc ttaacatcac aatcgccata tgattgaccg
    19621 gatcgataac ggttactgtc ccggtccagc cagtgtgacc gtaggtttgc gggcttgcca
    19681 gcgtgccaaa cgtcggcgtc atggtggcat taccattcac gcgccagccg aggccaaaag
    19741 tggcatcttc cttagagctg gtggtgaaca tcttcaccgt ttccgcactg aacagctgca
    19801 catcgccata gcccccgccg ttcagcatcg tttgcattaa caccgcaata tcgccggtat
    19861 tggaaaacaa acctgcgtgc ccggaaacgc cgcccatcga ataaaaggct ttttcatcgt
    19921 gcacctgacc ccagagagtg gaggtgcgga tattcgggaa atggatcacg ccatcgcggg
    19981 tattgccgtt caattccgtg gcggcaattt gttgcggttt aaagcctttc agcagcgggt
    20041 taaataccgt atgtgtcagg ccgagcgggc gataaatcga ctcttcaaca tagcggtcaa
    20101 gcggctgacc ggtaaccgac tcgacgataa atccaagcag catataatcg acatcgctgt
    20161 agatatgttt gctgccgggc tgatattcca gcggcgtgcg cttgatcatc tccagcgtct
    20221 ggcctttatc ctgggaatat aacgcgcccg cgaccgcttt attcgggtat tgcggatctg
    20281 ccgggaaacc gccgctgtga tgcagcaggt cagaaatccg cagggtgttt ttgcctttaa
    20341 tggtgtcatt tggactatcg gcaaaccccg gaatatattt cgcaatccga tcgtcaggat
    20401 gcagtttgcc ttcgctcatc agcttttgca gggcgaagtt cgtggcgtac attttggtgt
    20461 ttgaggccag gtcatacagc gtcccggtgg tggctttgac aggctgttcc atcaacacgc
    20521 tgccatcgta ctttttcgcc gctccccagg ctttgcgata cacaatctga ttatctttaa
    20581 tgatcagcag gtttacgccg ggataaccgg catcaacttg ctggctaatc cagcgatcca
    20641 tctggttaag ccgttcgacg ttaacccctg ctttctctgg cgagctttct gtcagaacag
    20701 ggtattttgc ggcgctgacg ctacaactaa cagccagcag agaaagatag agcattgtcc
    20761 gtttcatgat atcagtccag attgacgtta cggcagccaa agagcgtggt gaaaataaac
    20821 ccgcaaaccc atgccaccag aatcccgcca gcataaaccg ccatggccgg aaggatgcct
    20881 tgtgcggaag tcatcagcgg cagcgctacc agaccagacg gcccaaaggc gctgtttaag
    20941 cccatcggca gaccccacca ggctatcagg ccgataaaca aaccgcccgc cgcaccgcct
    21001 aaacaggcgg taacaaacgg cttcatgcgg ggcagggtga caccgtaaat cagcggttca
    21061 ccaacgccca gcaggccggg aataatcgcc ccgcgtacct gactgcgtag cgcactgtgc
    21121 ggttgcgccc gccagtagag tgccagcgcc gcgcccacct ggcccgcgcc tgccattgaa
    21181 aggatgggaa ataagctgtt gaatccctgg ctgtccatta acgcgaggta aacaggaata
    21241 aagccctgat gcacgccaaa taccacggcg atcaggaaca gcccggctaa aaccgcacaa
    21301 ccgaacggat tactgttcag gtgcataaac agccatgaca taccttcgaa taaccagcca
    21361 cccagcggca taatgatcag gtacgcaagc gtagcggtga tcagcagggt gattaacgag
    21421 gtcagcagca tgtcgagatc gtccggcata aagcggcgca ccatgccttc aatgcgggcg
    21481 caggcccacg cggcaatcag cacgccgata atattgccgc gcggatcgat gggcagacca
    21541 aagaaatcgt gaaaaccggc gtagtaaccg gtggttgcag cggggttata accgagcaaa
    21601 aagagtgcgg caataattgc gccattgacg cccgtgccgc caaatgcctg ggcggcgtta
    21661 tagcccacca gaatcaccag gaaagtgaac aaacctttgc tgaacacctt cataaaattc
    21721 agcgcgtcgg gtagcgtgcc ctgagcatct gccggaacgt gcatcaccgt ggcaattaac
    21781 gtcgctatcc ccagcagcag accggcggca ataaaaccgg ggatcagcgg cgtgaagatg
    21841 gtggcgaatt tggcgagaaa ttgttgcacg ccggaggttt gtttggcttt taactgacgt
    21901 ttgttctggg cggcgatttc tgcggcatcc tgtaccggtg cttctcccag cagctcgctc
    21961 atcgcttttg cggcacgatg cgctttacct ggcccaaaaa cgacctgcac ctggtcgctg
    22021 gtcaaaatga cgcccttcac gccttccagc gttttgatat tgggatcaac cagtgaactg
    22081 tcatgtacac ccagacgcag gcgcgtcata cagttaccac aactggcgat atttcccggt
    22141 ccgccgacac gggtaagaat ggtgttcaga agttcactgc tgatctcttt ggccatgggt
    22201 tattccttgt ctaaaacctg acgaataaag ccgccgtgtt gatccaggcg ttttttagct
    22261 tctgcggcat cgagattttt cagcaccatc acaatggccg ttttacagtt gcgctcgcag
    22321 gcaattaacg ccgcttccgc ttgttcggca ttacatccgg tggcgttttt aacgatattg
    22381 acctggcgca catgcagttt ttcgttggtg gcgaccacat cgaccatcag gttgccgaac
    22441 actttgccgg atttaatcat cagcccggtg gaaagcatat tgagcaccag tttctgcgct
    22501 gtgcctgctt tcattcgcga agaaccagtg accacttcag cacctacaat cggtgtaatg
    22561 gcaaactcgg cggtggtgga aacggcactc cccggattac aggaaattcc cactgtgcgg
    22621 caaccgagct ggcgggcgta ttccagtccg gcaatcacat acggcgtacg accgctggcg
    22681 gcaataccga ccaccacatc ctgcgcggtt aagccgatgt ttttcaaatc attaacaccg
    22741 ccttcccggc tatcttccgc gccttccacc gcgtgctgaa tggcatattc gccgccagca
    22801 atcaaaccaa ccaccagacc cggtttcacg ccgtaggtgg gcggacattc gctggcatcc
    22861 agaatcccca gacgaccgga tgttcccgca ccgaggtaaa tcagacgccc gccgccgctt
    22921 acctgggcgt ggataacatc gatcgacgcg gcgatatccg gcagtacgcg ctcaacggca
    22981 agcggtacgg ttttatcttc atcgttgata atccagcaca tttccagcgt tgatacgcgg
    23041 tcaatttcag ccgaggcggc gttcgagcct tcagtaatca tcttttcaag ttgcatcggt
    23101 ggtgacctta ctatttacga tcaaataatt gcagatgcat ggaatcatat atcccgaatt
    23161 gacagactta aaggaatcat tgagtctaca ctcgcagagt gtgaagagcg tcataagaac
    23221 ttaagcggaa tttttgctgc cagcccggga gagtggaaga tgttgtacct gacgaaaata
    23281 cgcaatgcgg aaagtgaatt taccgagaac gagcaaaaaa ttgctgactt tctgcgggcc
    23341 aacgtgagtg agctgaaatc cgtctcttcg cgcaagatgg cgaaacagct cggcatcagt
    23401 cagtcgagca ttgtgaaatt tgcccagaag ctcggcgcgc aaggatttac tgaattgcgt
    23461 atggcgttaa ttggcgaata cagtgccagc cgggagaaaa cgaatgccac ggcgctgcac
    23521 ctgcacagtt cgattaccag tgatgattcg ctggaagtga ttgcccgcaa actgaatcgc
    23581 gaaaaagagc tggcgctgga gcaaacctgt gcgttgttcg actacgcgcg gctgcaaaaa
    23641 atcattgagg tcatcagtaa agcaccgttt atccagataa cgggcctggg cgggtcggcg
    23701 ctggtggggc gcgatctgtc gttcaaactg atgaaaattg gctatcgcgt ggcctgtgaa
    23761 gccgacacgc acgtacaagc caccgtttcc caggcgttga aaaaaggcga tgtacaaatc
    23821 gctatctctt acagcggcag taaaaaagag attgtcttgt gcgcagaagc ggcacgaaag
    23881 cagggggcaa cggtgattgc cattacttct ctggctgact ctccgctgcg gcggctggcg
    23941 catttcacgc tcgataccgt ttccggtgaa accgaatggc gtagctcgtc gatgtctacc
    24001 cgcacggcgc aaaattctgt taccgattta ctgtttgttg gtcttgtgca actcaatgat
    24061 gtggagtcat tgaaaatgat tcagcgcagc agtgagctaa cacaacgcct gaaatgatgc
    24121 ataaagcagc gactggattg agattttcct gaattagtga gctgatccgc agcaatattt
    24181 tgtttatcct gtattttcag agggaatgga gtgtaacgct ctgtattaac aaggagagca
    24241 ttaaaatggg taaactcacg ggcaagacag cactgattac gggcgcattg cagggaattg
    24301 gcgaaggaat tgccagaact tttgcgcgtc atggcgcaaa cctaatcttg ctggatatct
    24361 cccctgagat cgaaaagctg gcggacgaac tgtgtggtcg tggtcatcgc tgtacggcgg
    24421 ttgtcgccga tgtgcgtgac ccggcgtcgg tagccgcagc tatcaaacgc gcgaaggaaa
    24481 aagaagggcg cattgatatc ctggtgaata acgcaggcgt ttgtcgtctg ggcagtttcc
    24541 tcgatatgag cgatgacgat cgcgatttcc atattgacat caatattaaa ggcgtatgga
    24601 acgtcacgaa ggcggtgctg ccggagatga ttgcccgcaa agatggtcgc attgtgatga
    24661 tgtcttcagt cactggtgat atggtggccg atcctggcga aacggcgtac gccttaacga
    24721 aagcggcgat tgttggcctg acaaaatcgc tggcggtgga gtacgcgcag tctggtattc
    24781 gcgttaacgc catttgcccg ggatacgtgc gcacaccaat ggcggaaagc attgcccgcc
    24841 agtcgaaccc ggaagatcca gagtcggtgc tgactgaaat ggcgaaagca atcccgatgc
    24901 gtcgcctcgc cgatccgctg gaagtcggcg aactggcggc cttcctcgca tcggatgaat
    24961 ccagctattt aaccggtaca cagaatgtga ttgatggcgg cagcacactg ccagagacgg
    25021 ttagcgtcgg tatctgattc acctctgttt cctccctgca tttgtgggga ggatttcgtt
    25081 ttaaacccgg ttcttattcc cttttcaact tccaaatcac caaacggtat ataaaaccgt
    25141 tactcctttc acgtccgtta taaatatgat ggctattaga aagtcattaa atttataagg
    25201 gtgcgcaatg gccgttaact tactgaaaaa gaactcactc gcgctggtcg cttctctgct
    25261 gctggcgggc catgtacagg caacggaact gctgaacagt tcttatgacg tctcccgcga
    25321 gctgtttgcc gccctgaacc cgccgtttga acagcaatgg gccaaagata acggcggcga
    25381 caaactgacg ataaaacaat ctcatgccgg gtcatcaaaa caggcgctgg caattttgca
    25441 gggcttaaaa gccgacgttg tcacttataa ccaggtgacc gacgtacaaa ttctgcacga
    25501 caaaggcaag ctgctcccgg ctgactggca gtcgcgcctg ccgaataata gctcgccgtt
    25561 ctactccacc atgggcttcc tggtgcgtaa gggcaacccg aagaatatcc acgactggaa
    25621 cgacctggtg cgctccgacg tgaagctgat tttcccgaac ccgaaaacgt cgggtaacgc
    25681 gcgttatacc tatctggcgg catggggcgc agcggataaa gctgatggtg gcgacaaagc
    25741 caaaaccgaa cagtttatga cccagttcct gaaaaacgtt gaagtgttcg atactggcgg
    25801 tcgtggcgcg accaccactt ttgccgagcg cggcctgggc gatgtactga tcagcttcga
    25861 gtcggaagtg aacaacatcc gtaaacagta tgaagcgcaa ggctttgaag tggtgattcc
    25921 gaaaaccaac attctggcgg aattcccggt ggcgtgggtc gataaaaacg tgcaggccaa
    25981 cggtacggaa aaagcagcaa aagcctacct gaactggctc tacagcccgc aggcgcagac
    26041 catcatcacc gactattact accgcgtaaa taacccggaa gtcatggaca aactgaaaga
    26101 taaattcccg cagaccgagc tgttccgcgt ggaagacaaa tttggctcct ggccggaagt
    26161 gatgaaaacc cacttcacca gcggcggcga gttagacaag ctgttagcgg cggggcgtaa
    26221 ctgatgtttg cagtttcatc aagacgcgtg ctgccgggct ttaccttaag cctcggcacc
    26281 agtctgctgt ttgtgtgcct gattttgctg ctgccgctct ccgcgctggt gatgcagctg
    26341 gctcagatga gctgggcgca gtactgggag gtgatcacca acccacaagt ggtcgcggcc
    26401 tacaaagtga cgctgctgtc ggcgtttgtg gcatcgattt ttaacggcgt tttcggtctg
    26461 ctgatggcgt ggattttaac acgttatcgc tttccgggcc gcacgctgct tgatgcgctg
    26521 atggatttac cctttgcgct gccaacggca gtcgccggtt taacgctggc ctcgctcttt
    26581 tccgtaaacg gtttttacgg tgaatggctg gcgaagtttg atatcaaagt cacctatacc
    26641 tggcttggta ttgcggtggc gatggccttt accagcattc cgtttgtggt gcgtaccgtg
    26701 cagccggtgc tggaagagtt aggcccagaa tatgaagaag cggcggaaac gcttggtgca
    26761 acgcgctggc agagtttctg caaagtggtg ctgccggaac tttctccagc gctggtggcg
    26821 ggcgtggcgt tgtcgtttac ccgtagtctt ggtgagtttg gcgcggtgat atttatcgcc
    26881 gggaacatcg cctggaagac ggaagtgacg tcgctgatga tttttgtgcg cttgcaggag
    26941 tttgattacc cggcggcgag cgcgattgct tcggtgatcc tcgcggcatc cctgctgctg
    27001 ctgttctcaa ttaacactct gcaaagtcgc tttggtcggc gtgtggtagg tcattaatgg
    27061 cggaagttac ccaattgaag cgttatgacg cgcgcccgat taactggggc aaatggtttc
    27121 tgattggcat cgggatgctg gtatcggcgt tcattctgct ggtgccgatg atttatatct
    27181 tcgtacaggc gttcagcaaa gggctgatgc cggttttaca gaatctggcc gatccggaca
    27241 tgctgcacgc catttggctg acggtgatga ttgcgctgat tgccgtaccg gtgaacctgg
    27301 tgttcggcat tctgctggcg tggctggtaa cgcgctttaa cttccccgga cgccagttac
    27361 tgctgacgct actggacatt ccgtttgccg tatcgccggt ggttgccggt ctggtgtatc
    27421 tgctgttcta cggctctaac ggtccgctgg gcggctggct ggatgaacat aatttgcaga
    27481 ttatgttctc ctggccggga atggtgctgg tcaccatctt tgtgacctgt ccttttgtgg
    27541 tgcgcgagct ggtgccggtg atgttaagcc agggcagcca ggaagacgaa gcggcgattt
    27601 tgcttggcgc gtctggctgg cagatgttcc gtcgcgtcac attgccgaac attcgctggg
    27661 cgctgctgta tggtgtggtg ctaaccaacg cccgcgcgat tggcgagttt ggtgcggtgt
    27721 cggtggtttc cggctcgatt cgcggcgaaa cactttcgct gccgttacag attgaattgc
    27781 tggagcagga ctacaacacc gtcggctcct ttaccgccgc cgcgctgttg acgctgatgg
    27841 cgattatcac cctgttttta aagagtatgt tgcagtggcg tctggagaat caggaaaaac
    27901 gcgcgcagca ggaggagcat catgagcatt gagattgcca atattaagaa gtcgtttggt
    27961 cgcacccagg tgctgaacga tatctcactg gatattcctt ctggtcagat ggtcgcgctg
    28021 ctggggccgt ccggttccgg aaaaaccacg ctgctgcgca ttattgccgg gctggagcat
    28081 caaaccagcg ggcatattcg cttccacggt accgacgtga gccgcctgca cgcacgcgat
    28141 cgtaaagtcg gtttcgtgtt ccagcattac gcgctgttcc gtcatatgac ggtgttcgac
    28201 aatatcgctt ttggcctgac ggtgctgcca cgtcgcgagc gcccgaatgc cgcagccatc
    28261 aaagcgaaag tgacaaaatt gctggagatg gtgcagcttg ctcatctggc agatcgttat
    28321 ccggcacagc tttccggcgg ccagaaacag cgcgtggcgc tggcgcgcgc gctggctgta
    28381 gaaccgcaaa ttctgctgct tgatgaaccg tttggcgcgc tggatgcgca ggtgcgtaaa
    28441 gagctgcgtc gctggctgcg tcaactgcat gaagagctga aattcaccag cgtgttcgtg
    28501 acccacgacc aggaagaagc gacggaagta gctgatcgtg tagtggtgat gagccagggc
    28561 aatattgaac aggctgatgc gccggagcag gtatggcgcg aaccagcgac ccgttttgtg
    28621 ctcgaattta tgggcgaagt gaaccgcctg cagggaacca ttcgcggcgg gcagttccat
    28681 gttggcgcac attgctggcc gctgggctac acacctgctt atcaggggcc ggtggatctc
    28741 ttcctgcgcc cgtgggaagt ggatatcagc cgccgtacca gtctcgattc gccgctgccg
    28801 gtacaggtac tggaagccag cccgaaaggt cactacaccc aattagtggt gcagccgctg
    28861 gggtggtaca acgaaccgct gacggtcgtg atgcatggcg acgatgcccc gcagcgtggc
    28921 gagcgtttat tcgttggtct gcaacatgcg cggctgtata acggcgacga gcgtatcgaa
    28981 acccgcgatg aggaacttgc tctcgcacaa agcgcctgat aggttgagtg aatgttaaac
    29041 gcccggaggc gcttcccgcg atccgggctt tttaatggca aggtttgtaa cctgtagacc
    29101 tgataagacg cgcaagcgtc gcatcaggca acaccacgta tggatagaga tcgtgagtac
    29161 attagaacaa acaataggca atacgcctct ggtgaagttg cagcgaatgg ggccggataa
    29221 cggcagtgaa gtgtggttaa aactggaagg caataacccg gcaggttcgg tgaaagatcg
    29281 tgcggcactt tcgatgatcg tcgaggcgga aaagcgcggg gaaattaaac cgggtgatgt
    29341 cttaatcgaa gccaccagtg gtaacaccgg cattgcgctg gcaatgattg ccgcgctgaa
    29401 aggctatcgc atgaaattgc tgatgcccga caacatgagc caggaacgcc gtgcggcgat
    29461 gcgtgcttat ggtgcggaac tgattcttgt caccaaagag cagggcatgg aaggtgcgcg
    29521 cgatctggcg ctggagatgg cgaatcgtgg cgaaggaaag ctgctcgatc agttcaataa
    29581 tcccgataac ccttacgccc attacaccac taccgggccg gaaatctggc agcaaaccgg
    29641 cgggcgcatt acccattttg tctccagcat gggaacaacc ggcactatca ccggcgtttc
    29701 acgctttatg cgcgaacaat ccaaaccagt aacgattgtt ggcctgcagc aggaagaagg
    29761 cagcagtatt cccggcattc gccgctggcc tgcggaatat ctgccgggga ttttcaacgc
    29821 ttctctggtg gatgaggtgc tggatattca tcagcgcgat gcagaaaaca ccatgcgcga
    29881 actggcagtg cgggaaggaa tattctgtgg cgtcagctcc ggcggcgcgg ttgccggagc
    29941 actgcgggtg gcaaaagcta accctggtgc ggtggtggta gcgatcatct gcgatcgtgg
    30001 cgatcgctac ctttccaccg gggtgtttgg ggaagagcat tttagccagg gggcggggat
    30061 ttaagattat tgcgcaggca ccgttttatc cgtctgcgca attagcgcat gtaaaaaagg
    30121 ctgtgcgttc tcatcgctct taccggggat cttcacaata tacggcttcc cggtaatcga
    30181 tgacgacgaa gcaaccttat caataaactg ctcggcagtg tcaatgcggt tacgggtatt
    30241 gcccagcttc agacgcagat gagaaaccgc ttcatcgcag gtatgttcat caccgttgcg
    30301 cacaaatatc aaatcctttt tctgtgctaa tccctccagc atggcgttga tgcgggcttc
    30361 ttcatgggca gttaacttcg cgtaagcggg gagtgtcatt agtagtgtaa tgaccagaca
    30421 aatgattttc ttcattgaat taccttatcc ttgttttgtg atagccgatt tgactgccag
    30481 aatcaggtaa agtttcggtt ggaatacgat gaggatcatc ggcaaggaga aaaaattaaa
    30541 tgagtagttt gttgttgttt aacgataaga gcagggcgct gcaggcggat atcgtcgccg
    30601 tgcagtcgca ggtggtttac ggcagcgtgg gcaacagcat tgccgtgcct gctatcaaac
    30661 agaacggtct gaatgttttt gccgtgccga cggtattgct gagcaatacg ccgcattatg
    30721 acactttcta cggtggtgcg attccggatg aatggtttag cggttatttg cgtgcgcttc
    30781 aggagcgtga tgcgctgcgc cagctgcgcg ctgtaaccac gggctatatg ggaacggcat
    30841 cgcaaatcaa aatccttgcc gagtggctga ctgcgctacg caaagaccat cctgacctat
    30901 tgatcatggt cgatccggtg attggcgata ttgatagcgg aatttatgtc aaacctgacc
    30961 ttcccgaggc gtatcgacaa tatttactgc cgctggcgca gggaattacc cccaatatct
    31021 ttgagctgga aatcctgacc ggtaaaaatt gccgcgatct cgacagtgcc attgctgccg
    31081 caaaaagtct gctttcagac acattaaaat gggtggtgat taccagcgcc tccggtaatg
    31141 aagaaaatca ggagatgcag gtggtggtgg tcagtgccga cagcgtgaat gtcatttccc
    31201 attcacgggt aaaaaccgac ctgaaaggga ctggcgacct gttttgtgct cagcttatca
    31261 gtggtttgct gaaagggaag gcgttaaacg atgcggtgca ccgtgcgggg ttgcgcgtac
    31321 tggaagtgat gcgctacacc cagcagcatg agagcgatga attgattttg ccgccgctgg
    31381 cggaagcata aaaaatggcg ccgatgggcg ccatttttca ctgcggcaag aattacttct
    31441 tgatgcggat aaccggggtt tcacccacgg ttacgctacc ggacagtttg atcagttctt
    31501 tgatttcgtc catgttggag ataacaaccg gagtcagggt agacttggct ttctcttcca
    31561 gtagcggcag atcaaattca atgacagtat cgccaacttt cacgcgctga ccttcttcag
    31621 caatacgctt gaagccttcg cctttcagtt caacggtgtc gataccgaag tggacgaaca
    31681 gttcaacgcc gctatcagat tcgatagaga acgcgtgatt ggtttcaaag attttaccaa
    31741 tggtaccgtc tacaggcgcg accattttgt tacccgttgg tttgatagca ataccatcac
    31801 caacgatttt ttccgcaaaa acgacatccg gcacgtcttc gatattgacg atctcgccag
    31861 agagcggagc aatgatctca atagttccgg tatccttctt gtcgtcggaa accagagatt
    31921 tcagtttatc gaacaaaccc atgatcttct cctaagcagt aaattgggcc gcatctcgtg
    31981 gattagcaga ttgttttttc ttcaatgaac ttgttaacca gcgtcattaa ctcgtccgtt
    32041 gtcggttgag caagagcctg ctctgctaac accttcgcat cttcgaagtt cgtgttacgg
    32101 ataatcttct taatgcgcgg gatagaaatg gcgctcatag agaattcgtc cagacccatc
    32161 cccagcaaca gaagtgtagc acgttcatcg ccagcaagct caccacacat gccagtccat
    32221 ttgccttcag catgagaagc atcaataact tgcttgatca agttcagcac ggacggtgac
    32281 attggttggt aaaggtgtga aatcatatca ttaccacggt caactgccag agtgtactgc
    32341 gttaaatcat tggtgccgat actaaagaaa tcaacttctt tggctaaatg acgtgcaatt
    32401 gttgcggcag ccggtgtttc caccattacg ccgatttcaa ttgactcgtc aaacgcttta
    32461 ccttcgtcgc gcagttcctg tttgtagatt tcgatctctt tgcgcagtgc acgcacttct
    32521 tcaacagaga tgatcatcgg gaacataatg cgcaatttac cgaaagccga ggcacgcagg
    32581 atagcgcgga gctgatcgcg caggatctct ttacgatcca tcgcgatacg gatagcgcgc
    32641 cagccgagga acgggttctc ttctttcggg aagttcatgt atggcagctc tttgtcgccg
    32701 ccgatgtcca tggtacgaac gataaccgcc tgcgagccac acgcttcagc cactgctttg
    32761 taagcagcaa actgttcttc ttcagtgggc agcgcgtcgc ggtccatgaa caggaactca
    32821 gtacgataca gaccaacgcc ttcagcgccg ttacgctctg caccttcaac gtcacgaacc
    32881 gtaccaatgt tagcgcacac ttctacctgg tgaccgtcca gcgtaatagc tggcagatct
    32941 ttcagtttag caagctctgc tttttcagaa gccacttgct cctgaacagc gcgcatttta
    33001 tcaataactt cgttggttgg attgacgtaa acctgattat ttacggcatc cagaatcaga
    33061 tagtcgtcat ttttcacctg agaggtgacg ctaccggtac ccacgatagc aggcagttcc
    33121 agagaacgcg ccatgataga ggtgtgggaa gtacggccac ccgcgtcggt gatgaaaccc
    33181 agcaccttct tcaggttcag ctgtgcggtt tcggacggcg tcaggtcagc ggcaaccaga
    33241 atgacttcat cctgaatggc gctcaggtcg ataatcttca ggcccaggat gttgcgcagc
    33301 aggcgcttac cgatatcacg tacgtcagcc gcacgttctt tcaggtattc atcatccagc
    33361 tcttccaggg cagaagcctg accttcgata acttcatgag cagctgcgtc agctgtcatg
    33421 tgcttatctt taatcagggc tatgatttcc tgctccagct cctcatcttc gagcagcata
    33481 atatgccctt caaagatggc ttctttttct tcaccgaacg tttcaccagc tttcgttttg
    33541 atcgtttcca gctgggctga tgccttggca cgaccgctca gaaaacgttc aacttcctga
    33601 tcaacctggt cggcagaaat ttttttccgg tcaatgacaa tttcgtcttc tttcagaagc
    33661 agagctttac cgaaagcgat acccggggat gctaaaatgc ctgaaatcat aaccctacct
    33721 tacttgtgac tgatttttaa aagaacccgg gaaattactc gagttccgcc atcagtttaa
    33781 ccagatgttc aaccgctttc tgctcgtctt cgccttctgc ggagatagtc acaacggtac
    33841 cttgagtcag gcccagagtc tgcagtttaa acaggctttt cgcgctggcg cttttgccgt
    33901 tggaagtcac agtaatttca gaagtgaagc ccttagcttc ttttacaaac tgggcagcag
    33961 ggcgggtgtg cagaccgttc ggagcggtaa tggtaacttc ttgctggaac attgtatttc
    34021 cccaacttat aggtttagtg ttgtggaact aaagtctagc ctggcggttc gactttagcc
    34081 tgtattgtta gcgccagttt ttaacagacg cgacgcacga agagcgcaat acagtccatt
    34141 gctggcgggt gttgaccgct gacgtttcgt acgtcattaa acattatgcc gctaaaggaa
    34201 gaattgaacc aaatcataaa atcgattcag ctttggcttt tcctgtaacg attaatttcg
    34261 cgcatcaaaa taattagtct ggttaaatac cagttccgac aggctgaatc aatgccagga
    34321 ggggcgaaag tttgaagcag gccacaaaaa agcacctaaa aaggtgcttt tttacgcatt
    34381 tttaacaagc tggcattact gttgcagttc tttctcagtg aagagatcgg caaacaatgc
    34441 ggtgcttaaa taacgctcac ccgatgacgg tagaataacc acaatattct tgttggtaaa
    34501 gctttcatct tcttgtagtt tcaacgccgc agcaacagct gctccagaag agatacctgc
    34561 aagaatacct tcttcttcca tcagacgacg cgcggtagaa atcgcttctt cattggtgat
    34621 gccaatgact ttatcgacca gcttgagatc gaggttagct gggataaaac cagcgccaat
    34681 accctgaatt ttatgcgggc caggtttaat ctcttcacca gccagtgcct gggcgataac
    34741 tggagaatcg gttggctcaa cggcgacaga gataagatcg gtcttgcctt tggtgccttt
    34801 aatgtagcgg ctgacgccag tcagcgtacc gccagtccca acgccagcaa taaatacatc
    34861 aacctgaccg tcggtatctt cccagatctc cggaccggtg gttttttcgt gaatttcagg
    34921 gtttgccgga ttgctgaatt gttgcagcag caggtatttc tctggattgc tggcgacaat
    34981 ttcttctgct ttttggattg cgcctttcat gcctttagca ccttccgtca gcaccaggtt
    35041 tgcacctaac gctttcagca gcttgcggcg ttcaatactc atggtttctg gcatggtcag
    35101 ggtgagtttg taaccgcgag cggcagctac ataggccagt gcaatcccgg tattaccgct
    35161 ggttggttca accagttcaa cgcctggttt cagcacgccg cgcttttcgg catcccaaat
    35221 catgttggca ccgatacggc acttaacgct gaagctgggg ttacgagatt ccaccttcgc
    35281 cagaatgcgt ccgttaccga tgcgattcag gcgaaccagc ggcgtgtgac cgatagtcag
    35341 cgagttatct tcaaaaatct tactcatggc ctgtccttaa ctgtatgaaa ttgggataca
    35401 acaggtagca tacccgctca gagaatatgc ggaagtaagg atttagcata tctatataca
    35461 gaagggaaat aatgacatgc aagatggaat aaggggcggc ataagccacc cctgtttcac
    35521 acaaacggtt tacaaaataa ccggtagatt gttaccgcca catcgcgtgt ttatcgcgat
    35581 agcaatcgac ccacatcgcc gtcgcgccac aaacggcaac gggcatgatg aacagattaa
    35641 gcagcgggat catcgtaaac aggctggtta aagcaccaaa ctgcatattg gtgattttgc
    35701 gtgtgcgcag ggcggtgcgc atctctttaa acggcacttt gtggttatcg aaggggtaat
    35761 cgcaatactg gatggctaac atccaggcgc taaacaggaa ccacagtacc ggcgcgacgg
    35821 tttgcccaat accagggatg aagtaaagaa ttagtaatac aattgcgcgc ggaagatacc
    35881 aggcaaattt ttgccattcg cgtttcatga ttcgcggcac atctttcatg ataccgaaaa
    35941 tcccggtatc tggcggtgta gcgccagtca atcgtgcttc cagttgttca gccaataaac
    36001 cgttaaacgg agcggcaatc cagttagcaa tcgtggagaa gaaatagcca aacactaaca
    36061 gcacagagat gacagccaga ggccacaaca gataactcag ccattgtagc cagtccggaa
    36121 cgtaactcat gagagtcggg atccagacat cgagctgtgt aaagagccac cagaatgcgc
    36181 cccccatcaa caaaatattg accagcagcg gtaaaataac gaaacgccga atcccaggtt
    36241 gcgagacgag cttccagcct tgcgcaaaat agtaaaaacc gctgcgtggg gcagatgtga
    36301 atgatgaaac cataatcagg atgagctcct tttgaccaat cccaggaaaa ttctgcgtat
    36361 tttaccgggt aattgcgcaa tggacagtta ggatatgttc gaaaaaacag caaaaagcac
    36421 gatttcatct atctttgtgc tgtgaaagtt aatagtgcac ttgcacttga ggtaatcggc
    36481 aaatactctt agtgagtaaa tgtttgccgt ggtggcaagg tgttagaaca acagagaata
    36541 taatgatgca ggatttgcgt ctgatattaa tcattgttgg cgcgatcgcc ataatcgctt
    36601 tactggtaca tggtttctgg accagccgta aagaacgatc ttctatgttc cgcgatcggc
    36661 cattaaaacg aatgaagtca aaacgtgacg acgattctta tgacgaggat gtcgaagatg
    36721 atgagggcgt tggtgaggtt cgtgttcacc gcgtgaatca tgccccggct aacgctcagg
    36781 agcatgaggc tgctcgtccg tcgccgcaac accagtacca accgccttat gcgtctgcgc
    36841 agccgcgtca accggtccag cagccgcctg aagcgcaggt accgccgcaa catgctccgc
    36901 gtccagcgca gccggtgcag cagcctgcct atcagccgca gcctgaacag ccgttgcagc
    36961 agccagtttc gccacaggtc gcgccagcgc cgcagcctgt gcattcagca ccgcaaccgg
    37021 cacaacaggc tttccagcct gcagaacccg tagcggcacc acagcctgag cctgtagcgg
    37081 aaccggctcc agttatggat aaaccgaagc gcaaagaagc ggtgattatc atgaacgtcg
    37141 cggcgcatca cggtagcgag ctaaacggtg aactgcttct taacagcatt caacaagcgg
    37201 gcttcatttt tggcgatatg aatatttacc atcgtcatct tagcccggat ggcagcggcc
    37261 cggcgttatt cagcctggcg aatatggtga aaccgggaac ctttgatcct gaaatgaagg
    37321 atttcactac tccgggtgtc accatcttta tgcaggtacc gtcttacggt gacgagctgc
    37381 agaacttcaa gctgatgctg caatctgcgc agcatattgc cgatgaagtg ggcggtgtcg
    37441 tgcttgacga tcagcgccgt atgatgactc cgcagaaatt gcgcgagtac caggacatca
    37501 tccgcgaagt caaagacgcc aacgcctgat acacttaagg caaattaact cctcttcgaa
    37561 cccccgcttg tcgggggttt ttagcattga tggtgcgata tggaatcaat cgaacaacaa
    37621 ctgacagaac tgcgaacgac gcttcgccat catgaatatc tttatcatgt gatggatgcg
    37681 ccggaaattc ccgacgctga atacgacagg ctgatgcgcg aactgcgcga gctggaaacc
    37741 aaacatccag aactgattac gcctgattcg cctacccaac gtgtaggtgc tgcgccgctg
    37801 gcagctttca gccagatacg ccatgaagta ccaatgctgt cactggataa cgtttttgat
    37861 gaagaaagct ttcttgcttt caacaaacgt gtgcaggacc gtctgaaaaa caacgagaaa
    37921 gtcacctggt gctgtgagct gaagctggat ggccttgccg tcagtattct gtatgaaaat
    37981 ggcgttttag tcagtgccgc gacccgtggc gatggcacca ccggggaaga tatcacgtct
    38041 aatgtgcgta ctattcgcgc cattccgctg aagctgcacg gagagaatat cccggcgcgt
    38101 ctggaagtgc gtggtgaagt gttcctgccg caggcggggt tcgaaaagat taacgaagat
    38161 gcgcgacgca cgggcgggaa agtttttgct aacccacgta atgcggcagc tggttcactg
    38221 cgtcagcttg atccgcgtat tacagcgaag cgaccgctca cttttttctg ctatggcgtt
    38281 ggtgttctgg aaggtggcga gctgccggat actcatcttg gccgtttact gcaatttaaa
    38341 aagtgggggt tgccggtcag cgatcgggta acgctttgtg aatcggcgga agaagtgctg
    38401 gcgttctatc acaaagtgga agaagaccgc ccgacgctgg gctttgatat cgacggcgtg
    38461 gtgattaagg tcaactcact ggaacagcag gagcagcttg gctttgtcgc gcgtgccccg
    38521 cgctgggcgg tagcgtttaa attcccggcg caggagcaga tgacctttgt gcgtgacgtc
    38581 gagtttcagg ttgggcgtac tggcgcgatt acgcctgttg cgcgtctgga acctgtccat
    38641 gttgctggcg tgctggtgag taacgcaacc ttacacaatg cggatgaaat cgaacgtctt
    38701 ggtttacgca ttggcgataa agtggtgatt cgccgcgctg gcgacgtgat cccgcaggtg
    38761 gttaacgtcg tgctttctga acgcccggaa gatacccgtg aggttgtatt cccgacgcat
    38821 tgtccggtat gtggttctga cgttgagcgt gtggaaggtg aagcggttgc ccgctgtacc
    38881 ggtggcctga tttgcggtgc gcagcgtaaa gagtcgctga aacactttgt ttcccgccgt
    38941 gcgatggatg ttgacggaat gggcgacaaa atcatcgatc agctggttga aaaagaatat
    39001 gtccatactc cggcagatct gttcaaactc accgcaggca aactgaccgg actggagcgt
    39061 atggggccaa aatcggcaca aaacgtggtt aacgcgctag aaaaagcgaa agaaaccacc
    39121 tttgctcgct tcctctatgc acttggcatc cgtgaagtcg gcgaggccac cgcagcaggt
    39181 ctggcggcat atttcggcac gctggaagcg ctggaagccg cttcgattga agagctgcaa
    39241 aaggtgcctg atgttggcat tgtcgttgca tcccacgttc acaacttctt tgccgaagaa
    39301 agcaaccgca atgtcatcag cgagctgttg gcggaaggtg ttcactggcc tgcgccgatc
    39361 gttatcaacg cggaagagat tgacagcccg tttgctggta aaaccgtggt gcttacgggc
    39421 agcttaagcc agatgtcgcg tgatgacgct aaagctcgac tggttgaact gggcgcgaaa
    39481 gtcgcgggca gcgtgtcgaa gaaaaccgat ctggtgattg ccggagaagc ggcaggttct
    39541 aaactggcga aagcgcagga actgggcatt gaagtcatcg acgaagcgga aatgctgcgt
    39601 ttgctgggta gctgagatgg aaaaagagca gctgattgaa atagccaata cgataatgcc
    39661 gtttggcaaa tataaagggc gtcgcttaat cgacctgccg gaggagtatc tgctgtggtt
    39721 tgcccgcaaa gatgaattcc cggcagggaa gctcggtgag ctaatgcaaa tcacgctgct
    39781 gattaaaacc gaggggctga cgcaactggt ccagcccctg aagcgtccgc tttaagcttt
    39841 atcggcgctg ctttcctgct gtgcctgtaa ctgttcggtc tggcgtttgt atcgacgcgc
    39901 cagcaccgca cagaccatca attggatctg atggaaaatc attaggggca gcaccatcat
    39961 accgatcacc gatgtgggga acagaatgtt tgccatcggg atgccatttg ccagactctt
    40021 tttcgaacca caaaagacga tagtaatttc atctgcctta ttgaagccca gtcggcgtgc
    40081 cataaagacg ttaactacaa tcacgatagc cagaagaacg cagctgacca ccacgataaa
    40141 cagcaatgat ccccagccaa ctttatgcca gataccatta acgacggctt cgctgaacgc
    40201 tgtataaacc accaacagaa tggacgtctg gtcagttttc gcaatccatt ttttattgcg
    40261 cgacacccag tcaccaatcc acggccggga aagatgcccc aacacaaacg gcagcagcag
    40321 ttgcagcata attttaccga cctgctcaag gctgccccct gcaccgtgaa cattcatcac
    40381 cagaccaacc agcaatggtg aaaggaaaat ccccagcagg ctggatgccg acgcagaaca
    40441 aaccgccgcc gcgacgttac cgcccgccat tgacgtgaag gcgattgcag actgcacggt
    40501 agccgggaga atgcacaagt agagaaaacc ggagtagagc atcgggtcga catttaccgg
    40561 tttccaccag gcaaacagta cacccagaat cggaaacagc acgaaggtgc tgcacattac
    40621 ccacaaatgc agtcgccagt gaccaccgcc agcaataatc gcctcacgcg acaacttcgc
    40681 gccgtgcata aagaacagca gggcaatagc tgcggtggtc agattttcaa agaaggggac
    40741 gaaatcgcct ctggccggaa agaaagaggc cagcaacacc accgtgatca gggttaaggt
    40801 gaaaggatcg aggatacgaa aaagtttcat aaagactcca gatgatcgat ggttctattg
    40861 tgcttcttcc agtttgagaa ataaaattga tttattgcat ccattcatga agaaaacaga
    40921 tgaattattc tttaaaacaa ttaaaggttt tcgtcacagt agcgcaggag aaaagtttta
    40981 gtcgtgcagg agagcgtatc ggcttgagcc agtcggcagt aagtcacagt gtgaaggagc
    41041 tggaaaatca tactggtgtt cgcctgctgg atagaaccac gcgtgaagtg gtgcttacag
    41101 atgcagggca gcagttggct ttgcgtcttg agcgactgct ggatgaactt aacagcacgt
    41161 tgcgcgatac cgggcgtatg gggcaacaac tgagcggaaa agttcgggtc gctgccagcc
    41221 agaccatttc cgcgcatctt attccgcaat gcattgctga aagccatcgc cgctatccag
    41281 atattcagtt tgtcctgcac gatcgtccgc agcagtgggt gatggaaagt attcgccagg
    41341 gagatgtcga ctttggcatc gtcatcgatc caggcccagt gggcgatctg caatgtgaag
    41401 cgattctttc cgagcctttc tttctgcttt gccatcgcga tagcgctttg gccgtggaag
    41461 attatgtacc ctggcaggca ttacagggag cgaaattggt gttgcaggat tacgcgtcag
    41521 gcagccgacc gctgattgac gcagcgctgg cgcgcaatgg tattcaggcg aatattgtgc
    41581 aggagattgg acatccggcg acgctgttcc cgatggtagc cgcaggcatc ggcatcagta
    41641 ttcttcccgc actggcgcta ccattgcctg aaggtagccc actggtggta aagcgcatta
    41701 cgccggttgt tgaacgccag ttgatgctgg tgcgcaggaa gaaccgttca ctttccaccg
    41761 ccgctgaagc gttatgggac gtggtacgag accagggcaa tgcgctaatg gctggtcggg
    41821 aaggggatcc gttgtatcag atatagatat cgatatgagt cgtattttca acaggattta
    41881 gaagggatac attcagagac aaacgctgct ctttctcctg cgccttttct gcctgctgtc
    41941 tctgcaactg cgccagttgt gtttccagta gggtgatttg ctgttgaatc aattcggcct
    42001 gtttttgttt gtcttcagca ctgccgctag tgtctacaat ctctttaatt tgttgagtga
    42061 gtttgatgat ttgctgactt atccgcgcga tttgtgaagc aacatcgcta ccttgtgatg
    42121 ccctgttact actctgtgtt tgtatggaag gaattgaagt tgaagagata gtggccattg
    42181 ttcagtcctc gtcactgtac gaggtaatat cggcagaaga acaaggaact tgagaagaaa
    42241 aatcaatctt acaaatcgca gatagcaaaa aagcgccttt agggcgcttt tttacattgg
    42301 tgggtcgtgc aggattcgaa cctgcgacca attgattaaa agtcaactgc tctaccaact
    42361 gagctaacga cccgaagtgg tgggtgatga cgggatcgaa ccgccgaccc cctccttgta
    42421 agggaggtgc tctcccagct gagctaatca cccacttcgg tacttcacat ttttacaaga
    42481 aatttagctg gcga
//
LOCUS       DAZKIV010000020        42374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZKIV010000020 DAZKIV010000000
VERSION     DAZKIV010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.020"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            204..830
                     /locus_tag="ACHOT6_001161"
     CDS             204..830
                     /locus_tag="ACHOT6_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFP9946414.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            851..2953
                     /locus_tag="ACHOT6_001162"
     CDS             851..2953
                     /locus_tag="ACHOT6_001162"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFP9946415.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKRRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLISSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            2966..3604
                     /locus_tag="ACHOT6_001163"
     CDS             2966..3604
                     /locus_tag="ACHOT6_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFP9946416.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            3668..4336
                     /locus_tag="ACHOT6_001164"
     CDS             3668..4336
                     /locus_tag="ACHOT6_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFP9946417.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4333..5118
                     /gene="phsC"
                     /locus_tag="ACHOT6_001165"
     CDS             4333..5118
                     /gene="phsC"
                     /locus_tag="ACHOT6_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFP9946418.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5122..5934
                     /gene="ydhT"
                     /locus_tag="ACHOT6_001166"
     CDS             5122..5934
                     /gene="ydhT"
                     /locus_tag="ACHOT6_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFP9946419.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(5946..7550)
                     /locus_tag="ACHOT6_001167"
     CDS             complement(5946..7550)
                     /locus_tag="ACHOT6_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFP9946420.1"
                     /translation="MKKIAIVGVGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLNTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(7676..7981)
                     /locus_tag="ACHOT6_001168"
     CDS             complement(7676..7981)
                     /locus_tag="ACHOT6_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFP9946421.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8089..8165)
                     /locus_tag="ACHOT6_001169"
     tRNA            complement(8089..8165)
                     /locus_tag="ACHOT6_001169"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8129..8131),aa:Val,seq:gac)
     gene            complement(8170..8246)
                     /locus_tag="ACHOT6_001170"
     tRNA            complement(8170..8246)
                     /locus_tag="ACHOT6_001170"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8210..8212),aa:Val,seq:gac)
     gene            8554..9810
                     /locus_tag="ACHOT6_001171"
     CDS             8554..9810
                     /locus_tag="ACHOT6_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFP9946422.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDSVTFSVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(9851..11224)
                     /gene="mdtK"
                     /locus_tag="ACHOT6_001172"
     CDS             complement(9851..11224)
                     /gene="mdtK"
                     /locus_tag="ACHOT6_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFP9946423.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVKPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            11439..12080
                     /gene="ribE"
                     /locus_tag="ACHOT6_001173"
     CDS             11439..12080
                     /gene="ribE"
                     /locus_tag="ACHOT6_001173"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFP9946424.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12120..13268)
                     /gene="cfa"
                     /locus_tag="ACHOT6_001174"
     CDS             complement(12120..13268)
                     /gene="cfa"
                     /locus_tag="ACHOT6_001174"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFP9946425.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13559..14770)
                     /gene="punC"
                     /locus_tag="ACHOT6_001175"
     CDS             complement(13559..14770)
                     /gene="punC"
                     /locus_tag="ACHOT6_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFP9946426.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            14883..15815
                     /gene="punR"
                     /locus_tag="ACHOT6_001176"
     CDS             14883..15815
                     /gene="punR"
                     /locus_tag="ACHOT6_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFP9946427.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            complement(15812..16837)
                     /gene="purR"
                     /locus_tag="ACHOT6_001177"
     CDS             complement(15812..16837)
                     /gene="purR"
                     /locus_tag="ACHOT6_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFP9946428.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17136..17225
                     /gene="cydH"
                     /locus_tag="ACHOT6_001178"
                     /gene_synonym="ynhF"
     CDS             17136..17225
                     /gene="cydH"
                     /locus_tag="ACHOT6_001178"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFP9946429.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17391..18560
                     /locus_tag="ACHOT6_001179"
                     /pseudo
     CDS             17391..18560
                     /locus_tag="ACHOT6_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(18706..19287)
                     /gene="sodB"
                     /locus_tag="ACHOT6_001180"
     CDS             complement(18706..19287)
                     /gene="sodB"
                     /locus_tag="ACHOT6_001180"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFP9946430.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19415..20230)
                     /gene="mepH"
                     /locus_tag="ACHOT6_001181"
     CDS             complement(19415..20230)
                     /gene="mepH"
                     /locus_tag="ACHOT6_001181"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFP9946431.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20564..20911
                     /gene="grxD"
                     /locus_tag="ACHOT6_001182"
     CDS             20564..20911
                     /gene="grxD"
                     /locus_tag="ACHOT6_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFP9946432.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(20962..25577)
                     /locus_tag="ACHOT6_001183"
                     /pseudo
     CDS             complement(20962..25577)
                     /locus_tag="ACHOT6_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(25670..26317)
                     /gene="rnt"
                     /locus_tag="ACHOT6_001184"
     CDS             complement(25670..26317)
                     /gene="rnt"
                     /locus_tag="ACHOT6_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFP9946433.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(26420..26827)
                     /gene="gloA"
                     /locus_tag="ACHOT6_001185"
     CDS             complement(26420..26827)
                     /gene="gloA"
                     /locus_tag="ACHOT6_001185"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFP9946434.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(26908..28005)
                     /gene="nemA"
                     /locus_tag="ACHOT6_001186"
     CDS             complement(26908..28005)
                     /gene="nemA"
                     /locus_tag="ACHOT6_001186"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFP9946435.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            complement(28042..28641)
                     /gene="nemR"
                     /locus_tag="ACHOT6_001187"
     CDS             complement(28042..28641)
                     /gene="nemR"
                     /locus_tag="ACHOT6_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFP9946436.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            28744..28983
                     /locus_tag="ACHOT6_001188"
     CDS             28744..28983
                     /locus_tag="ACHOT6_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFP9946437.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            29032..29928
                     /locus_tag="ACHOT6_001189"
     CDS             29032..29928
                     /locus_tag="ACHOT6_001189"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFP9946438.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            30009..30530
                     /gene="sodC"
                     /locus_tag="ACHOT6_001190"
     CDS             30009..30530
                     /gene="sodC"
                     /locus_tag="ACHOT6_001190"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9946439.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALSPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(30531..32543)
                     /locus_tag="ACHOT6_001191"
     CDS             complement(30531..32543)
                     /locus_tag="ACHOT6_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9946440.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(32543..33400)
                     /locus_tag="ACHOT6_001192"
     CDS             complement(32543..33400)
                     /locus_tag="ACHOT6_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFP9946441.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(33403..33639)
                     /locus_tag="ACHOT6_001193"
     CDS             complement(33403..33639)
                     /locus_tag="ACHOT6_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFP9946442.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            33840..34274
                     /gene="slyA"
                     /locus_tag="ACHOT6_001194"
     CDS             33840..34274
                     /gene="slyA"
                     /locus_tag="ACHOT6_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFP9946443.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(34321..34788)
                     /gene="slyB"
                     /locus_tag="ACHOT6_001195"
     CDS             complement(34321..34788)
                     /gene="slyB"
                     /locus_tag="ACHOT6_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFP9946444.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            35062..36171
                     /gene="anmK"
                     /locus_tag="ACHOT6_001196"
     CDS             35062..36171
                     /gene="anmK"
                     /locus_tag="ACHOT6_001196"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFP9946445.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVEPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNLLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            36269..36598
                     /gene="mliC"
                     /locus_tag="ACHOT6_001197"
     CDS             36269..36598
                     /gene="mliC"
                     /locus_tag="ACHOT6_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFP9946446.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            36657..37313
                     /gene="pdxH"
                     /locus_tag="ACHOT6_001198"
     CDS             36657..37313
                     /gene="pdxH"
                     /locus_tag="ACHOT6_001198"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFP9946447.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            37442..38716
                     /gene="tyrS"
                     /locus_tag="ACHOT6_001199"
     CDS             37442..38716
                     /gene="tyrS"
                     /locus_tag="ACHOT6_001199"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFP9946448.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            38775..39638
                     /gene="pdxY"
                     /locus_tag="ACHOT6_001200"
     CDS             38775..39638
                     /gene="pdxY"
                     /locus_tag="ACHOT6_001200"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFP9946449.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(39682..40287)
                     /gene="gstA"
                     /locus_tag="ACHOT6_001201"
     CDS             complement(39682..40287)
                     /gene="gstA"
                     /locus_tag="ACHOT6_001201"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFP9946450.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(40393..41895)
                     /gene="dtpA"
                     /locus_tag="ACHOT6_001202"
     CDS             complement(40393..41895)
                     /gene="dtpA"
                     /locus_tag="ACHOT6_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFP9946451.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT        10706 a        10418 c        11067 g        10183 t
ORIGIN      
        1 aatgttaagg gctcagaata atcacattat tttttctcct aaccataaag gattagttta
       61 tcggcgttaa atcttttctc atacactatc gggttaggga aaatatctgg tgttggggac
      121 agagtcagca gcgtattagt tgaatgctta atatcaggct gctgaattgt cagcactgtg
      181 tttgcgaaga ggatgcgaaa taaatgaacc cggttgatcg tccactatta gatattggct
      241 taacgcgatt agaattcttg cgtatatcag gaaaaggcct tgctggtcta acaattgccc
      301 ctgcgttact gtcacttttg ggttgcaaac aggaagatat tgatagcggc acagtagggt
      361 tgataaatac acccaaaggg gtgctggtga cccagcgagc gcgatgcacg ggttgccacc
      421 gctgtgaaat ctcttgtacc aacttcaacg atggctcagt agggacattc ttctcccgta
      481 tcaaaatcca tcgcaattat ttctttggcg acaacggggt tggctctggc ggcggcctgt
      541 atggcgatct caactatacc gcggacacct gccgtcaatg caaagagccg caatgcatga
      601 acgtctgccc gattggtgcg attacctggc agcagaaaga aggctgtatt accgtcgatc
      661 ataaacgctg tattggctgt agcgcctgta ctacggcatg cccgtggatg atggccaccg
      721 taaataccga aagtaaaaaa tcctcgaaat gtgtgttatg cggtgaatgc gcaaacgcct
      781 gcccaacagg ggcgttaaaa attatcgagt ggaaagatat tactgtgtga atcttgcaaa
      841 ggaaaaaatc atggctaacg gttggacagg taatatatta agagtcaatc tcacgacagg
      901 aaatattacc ctcgaagatt ccagtaagtt taaaagtttt gtcggtggca tgggcttcgg
      961 ctacaaaatt atgtatgacg aagtaccgcc aggcacgaaa cctttcgatg aagcgaataa
     1021 attagtcttt gctaccggcc cattaactgg atctggtgcc ccctgtagtt ctcgcgtaaa
     1081 tatcacctca ctttctactt ttaccaaagg aaatttagtt gtcgatgccc atatgggtgg
     1141 cttttttgca gcgcaaatga aattcgctgg atacgacgtc attattatcg aagggaaggc
     1201 gaaatcaccg gtatggctga agattaaaga tgacaaagtt agcctggaaa aagccgattt
     1261 cttatgggga aaagggacgc gcgcaacgac ggaagaaatt tgtcgattga ccagtccgga
     1321 aacctgtgtg gcggctattg gtcaggctgg ggaaaacctt gttcctctct ctggcatgtt
     1381 gaatagccgt aaccacagcg gcggtgcggg aactggcgca attatgggtt cgaaaaacct
     1441 gaaagcgatt gcggttgaag ggacgaaagg ggtcaacatt gccgatcgtc aggagatgaa
     1501 gcgtctcaac gattacatga tgactgaact tattggtgcg aataacaacc atgtcgtgcc
     1561 aagtacgccg caatcgtggg cagagtattc agatcccaag tcacgttgga cagcacgtaa
     1621 agggctgttt tggggcgcgg ctgaaggtgg tccgattgaa acgggggaaa ttccgccagg
     1681 caatcagaac acggtcggct ttcgtaccta taaatccgtt tttgacttag gtccggcggc
     1741 agagaaatac acagtaaaaa tgagtggctg ccactcttgc ccgatccgtt gtatgaccca
     1801 aatgaatatt cctcgggtga aagagtttgg cgtgcccagc acaggtggta acacttgtgt
     1861 agccaacttt gtccatacca ccatcttccc gaacggtccg aaagattttg aagataaaga
     1921 cgatggtcgt gtgattggta acctggtggg tctgaatctg ttcgatgact acggcctatg
     1981 gtgtaactac gggcagttgc atcgcgactt tacctattgt tacagcaaag gtgtgttcaa
     2041 gcgtgttctg cctgctgaag agtatgcgga aattcgctgg gatcaactgg aagcgggtga
     2101 cgttaacttc attaaagatt tttactaccg tctggcgcat cgtgtgggtg agctgagcca
     2161 cctggctgat ggttcatatg ccatcgcaga acgctggaat ttgggtgaag agtactgggg
     2221 ctacgcgaaa aataaactct ggtcgccgtt tggctatccg gttcaccatg ccaatgaagc
     2281 gtcagcgcag gtcggttcca ttgttaactg tatgttcaac cgtgactgca tgacgcatac
     2341 ccatatcaac tttattggtt ccggcttgcc attgaaactg caacgtgaag tggcgaaaga
     2401 gctttttggt tctgaagatg cctacgatga aaccaaaaac tacacgccaa tcaatgacgc
     2461 aaaaattaaa tatgctaagt ggtcgctgtt gcgggtctgt ttgcataacg ccgtcaccct
     2521 gtgtaactgg gtctggccaa tgaccgtttc gccgctgaaa agacgtaatt accggggcga
     2581 tctggcgctt gaagccaaat tcttcaaagc gatcaccggc gaagagatga ctcaggaaaa
     2641 attagattta gctgcagagc gtatctttac gttgcatcgt gcctacacgg taaaactgat
     2701 gcaaaccaaa gatatgcgta atgaacacga tcttatcagt tcctgggtat tcgacaagga
     2761 tccgcagatc ccggtcttta ctgaaggtac tgacaaaatg gatcgtgacg atatgcatgc
     2821 ttcgctcacg atgttctaca aagaaatggg ctgggaccca cagcttggtt gtccaacccg
     2881 cgaaacattg cagcgtctgg ggctggaaga tattgccgcc gatctggcag cacacaatct
     2941 actgcctgcg taaggaatgg gtaagatgaa ccatcaggat gaattgccgt tagcgaaagt
     3001 cagcgaagtt gacgaggcga agcggcaatg gttgcagggg atgcgtcacc ccgttgatac
     3061 ggtaaccgaa ccagaacctg ctgaaatcct ggctgagttc attcgccagc attctgcggc
     3121 tgggcagctg gtggcgcgca ccgtgtttct ctcgccaccc tattcggttg cagaagagga
     3181 actgtcagtt ttgctggaaa gcattaagca gaacggagac tacgcagata tcgcttgtat
     3241 gacgggaagt caggatgatt actattactc aacgcaggcc atgagtgaaa actacgcggc
     3301 gatgtctttg caagttgtgg agcaggacat ttgtcgcgcc attgcacacg ccgttcgttt
     3361 tgagtgccag acatatcctc gtccttacaa agtcgccatg ctgatgcaag cgccatatta
     3421 cttccaggaa gcgcagattg aagccgcgat cgccgcgatg gatgttgcac ctgaatatgc
     3481 cgatatacgg caggtggagt catcgacggc ggtgttgtac ttgtttagcg agcgatttat
     3541 gacctatggc aaggcttatg gcttatgcga atggttcgag gtagagcagt ttcagaaccc
     3601 ctgaaatttc agggagatgt catggatagg gtggacagtt gcagccactg cattagggga
     3661 taatcagatg tccttcactc gacgcaaatt tgttctgggg atgggaacag taatcttttt
     3721 tactggttct gcgtcttctc tgttagcgaa cacgaggcaa gaaaaggaag ttcggtatgc
     3781 catgattcat gacgagtcac ggtgcaatgg ctgtaatatt tgcgcccgcg catgccgtaa
     3841 aactaaccac gtccccgctc agggaagccg tttatcgata gcacacattc cagtcaccga
     3901 taacgacaac gaaacgcagt atcacttttt tcgccagtca tgtcagcact gcgaagatgc
     3961 tccgtgcatc gacgtatgcc cgacaggggc gtcgtggcgt gatgagcagg ggatcgtgcg
     4021 ggtagaaaaa tcgcagtgca tcggctgcag ttattgcatt ggcgcttgtc cgtaccaggt
     4081 tcgctacctt aaccctgtca ctaaagtggc agataaatgt gatttctgcg ctgagtcccg
     4141 actggcaaaa ggctttccgc ccatttgcgt cagcgcctgt ccggaacacg cattgatctt
     4201 tggtcgcgaa gatagcccag aaattcaggc gtggttgcag gataataaat actatcaata
     4261 tcagctacct ggtgccggga aaccgcatct gtatcgccgg ttcggtcaac atttgattaa
     4321 aaaggaaaat gtatgaaccc gtcgcaacat gctgaacagt ttcagagcca gttagcgaac
     4381 tatgtgccac agttcactcc cgaattttgg ccggtgtggt tgatcattgc cggagtattg
     4441 ctggttggga tgtggttggt gctggggctg cacgccttgc ttcgtgctcg tggcgtgaag
     4501 aaatcagcca ccgatcatgg tgagaaggtt tatctttaca gcaaagcggt cagattatgg
     4561 cactggtcga atgcgttact ctttgtattg ttgctggcaa gtgggctgat aaatcacttt
     4621 gcgatggtgg gcgcaactgc ggttaaaagt ctggttgcgg tgcatgaagt ttgcggattt
     4681 ttgttactgg catgctggct cggctttgtg ctgatcaatg ccgttgggga taatggtcac
     4741 cactatcgca ttcgtcgtca ggggtggctg gaacgagcgg caaaacaaac gcgattttat
     4801 ttgtttggca ttatgcaggg ggaagaacat cctttcccgg caacaacaca gtctaaattt
     4861 aatcccttac agcaggtcgc ctatgttggt gtcatgtatg gattgctgcc gttgttacta
     4921 ttgacggggc tgttgtgtct ctatccgcaa gccgtgggag atgtgtttcc tggcgtaaga
     4981 tactggttat tgcaggcgca ttttgctctg gcatttataa gcctcttttt tatcttcggt
     5041 catctttatc tttgcaccac ggggcgtacg ccacacgaaa cctttaaaag catggtcgat
     5101 ggctatcacc ggcactaacg aatgatcatt acacgagcag acctgcgaga atggcgtatc
     5161 ggagcggtga tgtatcgctg gttcctgcgc catttcccac gcggcggtag ttatgccgat
     5221 attcatcatg cactgattga ggaaggatat acggactggg cggagagtct ggttgagtat
     5281 gcgtggaaaa aatggctggc ggatgaaaac ttcgcgcatc aggaagtttc ctctatgcaa
     5341 aaactggcca cagaccccgg agagatacca ttttgcagtc agtttgcccg cagcgacgat
     5401 catgctcgca tcggctgttg tgaggataac gcccgaatcg caaccgctgg gtatgccgcg
     5461 caaattgcca gtatgggtta tagcgtgcgg attggaagtg tcggctttaa cagtcacatt
     5521 ggcagctccg gtgaacgcgc acgggttgct gtaaccggta attcttcgcg gataagcagc
     5581 gctggagata gtagtcggat tgcgaatacc ggaatgcgag tacgcgtttg tacattgggt
     5641 gaacggtgtc atgtcgccag taatggtgac ctggtgcaaa tcgccagctt tggcgcaaac
     5701 gccagaattg ccaacagcgg cgacaatgta cacatcattg ccagcggtga aaattcaacg
     5761 gtagtgagca cgggcgtggt cgactcaatt attctcgggc cagggggaag cgccgcactg
     5821 gcatatcatg acggcgaaag agttcgcttc gccgtcgcca ttgaaggtga aaacaatatt
     5881 cgaaccggag tccgctatcg tcttaatgaa cagcatcagt ttgttgagtg ctgatagctt
     5941 tacctttaat caaacgaaag acgccgacga gcacgggatg caggctttac gaccgcccga
     6001 gccatcgcct caccaatttc tgcacatgcc gtaagtccct gaacgaaagg ctggtcgtgc
     6061 atcaaccagg gcaacgcgcc gaacgctacg cgtccacgaa tatcttcggg ttgctgtaac
     6121 gtataatctt cgccaacatc agggatttca tcccctgttt tctgtaattg ctcgcgtagc
     6181 ccagggaaag gaatatcttt cactttaagc ggacgctgtc cgcgggcatc aataaaaacg
     6241 tcaaacgagt agctgttgtc ttccgtattc aggacggtgc gcgactcatt aatttccatt
     6301 ttgtagtctt caccgagggc gagaatatga atgattcccg cttcacgtaa ggcaagtagg
     6361 cgacgaatag actctgaagg gattgccgca taattatcga tgaaaaccct ggcaaggcct
     6421 ttactgaacc gtttatggtc gtgttcattc agatgaggaa caatttcctg tacggcttca
     6481 tgcaggcgca gaatgacata acgccaggga acagtatgtt tttctcgttt attgcgttcg
     6541 acttcctgga gatttttttc tgcccagtca aatgggtcgc gttgtttgcg ctcggcaaac
     6601 caggcttgag caaaggaatc gacattcagg ctctctaagg ctatgcgttg gctccagtct
     6661 ggatcagcaa acttgatttc ctctactatc aatctaaata cccgatccaa aaggccctct
     6721 tcaccttttt gaatctcagc atttaatgcc tgatcggtga cgatgtgtaa gggctcgtag
     6781 ggaatagggc aatagaaatc ggcttcgggt aaaatacccg tgcgcgacat caacgtaata
     6841 tttagctttt cacttgcgtt atcgcggtta aaaacgacgt gttgtttatc atcttcaatg
     6901 aacgaaccat gctgaatagc cactgccatt gccgcatcca gtccgctcaa ggatgttccc
     6961 ataataccca cgttacacgc atcgaccttt gcttccatca ggcctgacca cggactggga
     7021 aaatacgttc tggttgcttc ttcttcatca ggccagacgt gacccgtggc aatcaccgct
     7081 aaatcaaacg tctcgctggg taaatcctga tttgtagcga gcatgacgcc agcatttgta
     7141 atttgcagat cggtaacctg gcatgattca taaacagcca ctgcaaattt ttgctgtcgt
     7201 gcctggtcta ctaatcgtaa aaattgatcg cggaaatatt cgcccagcag aattcgcggt
     7261 aaaaactgac gatcgtgcaa ggtttctttt ttaacgccat aacgctggag atggctggct
     7321 tcttgctttt gtagccattc gagatacgta caattaatcg gcggtatttc aatactggca
     7381 atatttgcca gcatcatttt tgagttttcc tcatcactgt atggcatccc gacaccggcc
     7441 tcgtcagcct gctcgaagat agaaatagaa agtggagttt gttgctgtag aagcgagaat
     7501 aaggtgtaga tccccgtagg cccgacaccc acaatagcaa tttttttcat tgttacttac
     7561 ctgtacgcat tctttcgaat ttagtgttat cagataagtt tggttgagat ttttagaatt
     7621 tgagaaattt cagacaggga tgaaaagggc gctaaccgcc cttgattaaa ttctgtcagg
     7681 cgagttttgc ctgattgatt tgactcagtg gctcattgac atcgaaaact ttggcgacaa
     7741 cttcctcaac gccgagattt ttaagtcggg cagtatgttt ttccagatag gcaagggcgc
     7801 ttttttcatc agtaaaaaga tagatcccac cggcttcgtg gttcttttca ctttctgtcc
     7861 ataccttcca cagaaaacca ggttcctgat taatcgactc agcaagcggt ttaagctgct
     7921 cagccattgc gtcgccaaat gggccattaa aagcaaaatg aagttgtaac aaagttgcca
     7981 tagcgattct ccttctgata acgcatacga agacagtgta aatcttttaa cgtattggat
     8041 acagagaaga aacagaggga aaaaggagaa gaccagatta agaaaatctg gtgcgtccga
     8101 gtggactcga accaccgacc cccaccatgt caaggtggtg ctctaaccaa ctgagctacg
     8161 gacgcaggat ggtgcgttca attggactcg aaccaacgac ccccaccatg tcaaggtggt
     8221 gctctaacca actgagctat gaacgcaacg ttgtaggtga caacggggac gaatattagc
     8281 ggcagagtgg gaaggtggca agaggcaaaa cgtaattttc tgcgctattt cgaccgtttg
     8341 cagagctttt aagcaaattg gctatatttt gttgatttgc aagggcgatt tttattcagg
     8401 atcgcattta catctgatac aacccaataa ttcgtgtatc agagtttaaa cagagttgtt
     8461 tgccaccatc tcaaatctgt cgtggataaa tcctccgtag cctttacact taggtagcaa
     8521 taaaggcaca cagatgcagc aaaggaataa atcatgggat ctgatgcgaa aaacttgatg
     8581 agcgacggga atgtgcaaat tgttaagacc ggcgaggtca ttggcgcgac gcaacttact
     8641 gaaggcgagt tgattgttga agctggcgga agagccgaaa ataccgtggt cacgggggct
     8701 ggctggttga aagtggcaac cggtgggatc gccaaatgca cacagtacgg taacaatggc
     8761 acgctatcgg tcagcgacgg tgccattgcc acagatattg ttcagtccga gggaggcgca
     8821 attagtctct ctacgctcgc tacggttaat ggccgccatc ccgaaggtga attcagcgtt
     8881 gatcagggtt atgcctgcgg tttgttgctg gaaaatggcg gtaacctgcg tgtactggaa
     8941 ggacatcgcg cggaaaaaat cattctcgat caagagggcg gcctgttggt taatgggaca
     9001 acctcagcgg tcgtggtaga tgaaggtggt gaattgttgg tgtatccagg tggggaagcc
     9061 agcaattgtg agattaatca gggcggcgtt tttatgctgg ccgggaaagc cagtgatacg
     9121 ttgcttgctg gtggcaccat gaataatctc ggtggtgaag actctgacac tattgttgag
     9181 aatggatcca tttatcgtct ggggacggat ggccttcagc tctacagttc cggtaagacg
     9241 caaaacctgt ccgtgaatgt gggtggtcgg gctgaagtgc atgccggtac gctggaaaat
     9301 gcggtaatac aaggtggaac agtgatcctg ttgtcaccca ccagcgcgga cgaaaatttt
     9361 gtcgtagagg aagatcgcgc accggttgaa ctgaccggga gtgttgcatt actggacggc
     9421 gcttcaatga ttattggcta tggcgcagat ctgcaacaat caacgattac tgtacagcag
     9481 ggcggtgtgt tgattctcga cggcagtacg gtaaaaggtg acagtgtcac ttttagtgtt
     9541 ggtaacatca atctgaacgg cggaaaactg tggctgatca ctggtgcggc aacgcatgtg
     9601 caactgaaag tgaaacgcct gcgcggagag ggagcgattt gcctgcaaac cagtgcgaaa
     9661 gaaatctcac ctgacttcat caatgtgaaa ggggaagtta ccggggatat acacgttgag
     9721 ataacagatg ccagtcggca aactctgtgc aacgctctga aattacagcc agacgaagac
     9781 gggattggcg caacgctcca gcctgcgtaa aaacacaggc gcaacccgtg aaggttgcgc
     9841 cttgtcttta ttagcgggat gctcgttgca gaatgataac tgacggcaga cgttgcagga
     9901 accgcatacg cagcatcatc ataatggctg ccgacgtcag gccaataata aagcctatcc
     9961 agaagcctgc tggccccata ggtttaacga ccagatcggt cagtgccaga atatagccgc
    10021 ttggcaagcc cagcacccag taagccgtaa aggtgatata gaaaatggaa cgcgtatctt
    10081 tataaccacg caaaatccca ctgccaatca cctggattga gtcagaaatc tgatataccg
    10141 ccgccagcaa catcaaatgc gcagccagcg ttacaacctc gggattgtcg ttgtacaaca
    10201 gggcgatttg ctcccgcagt gaaaccgtga atatggccgt cagggttgcc atacagacac
    10261 ccaccataag cccggtccgc gcagcggttt gcgcatccag cgttgagccc tgacccagac
    10321 gataacctac gcggatagtt accgctgccg ccagcgacat tggaagcaca aacatcagtg
    10381 aactaaagtt cagggcaatc tggtgtcctg cgacatcaac aataccgagc ggagacacta
    10441 acagagccac gacggcaaac agtgtcactt caaagaatag tgccagcgca atcggcaaac
    10501 cgagttgaat cagtcgtttc ataaccgcag gatcgggttt cgcggtgcct ttttcgttac
    10561 gaatatcgcg catggagcgg gcgcgtttaa tgtaagaaac catggcaagg aacatgaccc
    10621 aatacaccgc cgcagtagcc acgccacaac caacgccacc gagctcaggc ataccgaaat
    10681 gaccataaat aaagatatag ttcaccggga tgttcaccag caggccgata aagcccatta
    10741 ccatacccgg cttggttttt gccagacctt cacattggtt acgggcaacc tggaagaaaa
    10801 gatagcctgg cgcgccccac agcaacgcac gcagataacc cacggctttg tccgccagag
    10861 ccggatcgat gttttccatt gagcggataa tgtaacctgc attccacagc accagcataa
    10921 tgaggacgga aacaaaacct gccagccaga agccctgtcg cacctgatgc gcaatgcgct
    10981 cacgtcgacc ggaaccattt aattgcgcga taaccggcgt taatgccagc agcagtccgt
    11041 gaccaaagag gatcgccgga agccagatag aagtaccgat agcgaccgcc gccatgtcgg
    11101 tggcactata gccgcccgcc atcacggtat cgacaaaacc catcgcagtt tgggcgattt
    11161 gcgcgagaat caccgggatt gctaatgcta ataacagacg cgcttcactg atatacttct
    11221 gcacgtgaac accttttatt tgtagttata tgagagacta aaaagccgcc tgaatgggca
    11281 gcaaaaagaa gaagcagggg aaattcagtc tattgtagcg aggatttatt aattctccag
    11341 tgaaaaaata gtcattctta ccccttcact ggtaagggcg tttgccacct gctattgtgc
    11401 tgaacagaat gtcttaactg atttcaggag ttgtaagtat gtttacgggg attgtacagg
    11461 gcaccgcaaa actggtgtcg attgacgaga aaccaaattt tcgtacgcat gtggtggagt
    11521 tacccgacca catgctggac ggcctggaaa caggtgcttc cgtggcgcat aacggttgct
    11581 gcctgaccgt gacggaaatt aacggcaacc atgtcagttt tgacctgatg aaagaaacgt
    11641 tacgcattac caatcttggc gatttaaaag tgggggattg ggtaaacgtt gagcgtgcgg
    11701 cgaaattcag tgatgaaatt ggcggacact taatgtcagg tcatattatg accaccgctg
    11761 aagtggcgaa aatattaacc tcggaaaata atcgccagat ctggtttaaa gtccaggata
    11821 gtcagttgat gaaatatatc ctgtacaaag gatttattgg catcgacggt attagcctga
    11881 ccgtcggcga agtcacgcca acgcgttttt gtgtccattt aattccggaa actctggaac
    11941 gcacgactct tgggaagaaa aaactcggcg cacgcgtcaa cattgaaatt gatccacaaa
    12001 ctcaggcagt ggtagatacg gtagaacgtg tgctggcggc acgagaaaat gccatgaatc
    12061 aaccaggtac agaagcctga tctgaaatcg cccccggaga gggggcgata gaatagcctt
    12121 tagcgagcga ctcgaaggcc gttttcaaca ccgcgtgaga acacgacctg ccagagctga
    12181 atatcacggg cgcggaaagc acctgcacag gcattcagat aataggtaaa cattcgttta
    12241 aagcgttcac tgtagttatc cgcaatttct ggccatgcgg cgaggaatcg ttcataccac
    12301 gccatcaacg tagtatcgta atcagcaccg aagttatgcc agtcttccat cacgaagtgg
    12361 ggttcgctgg attgagcaat ctggcgtaca gagggcaggc aaccgttcgg aaaaatatat
    12421 ttattaatcc aaggatcaac attcagatcg gtttttttcg aaccgatagt atggagcagg
    12481 aatatacctt ccggtttcaa attacgatcc accaccgcaa aataggtatc gtaatttttc
    12541 ggtccgacgt gctcgaacat ccccacagaa acaatacgat caaactggtc gttcaggtca
    12601 cgataatctt gcagcaaaat ggtgacatcc aggccttcac agcgttcctg agccattttt
    12661 tgctgttcgg cagaaatggt gacgccgacc acgcttacgt catagttaga tgccatgtag
    12721 tgcgccagtc cgccccagcc gcagccaata tccagtacgc gcatccctgg ttttaactgc
    12781 aatttttcac aaatcatttt gagcttcgcc tgctgggcag attccagatt atcggcatct
    12841 ttccagtaag cgcaggaata ttgcatgaag ggatcaagca tgcggctgaa caagtcatta
    12901 cccaaatcgt aatgctcctt gccgactatc caggcacgtt ttttactctg cagattgaag
    12961 agacgagcgc tggcaatacg cagcgtgtct ttgaaatgat gggggagttg gttctcgaga
    13021 cctgcgcgta agactttgct aaaaaacata tccagtcggt cacattccca ccagccatcc
    13081 atataacttt cgcctaaccc caaagagcct tcttgcagaa cgcgtttaaa aaaatcgggg
    13141 tttttcacac gaatatccgc cggggcagaa ccgttaatgg ctataccggc acggctaagt
    13201 aattcgttgg cgatacggta ccagttgtca tccggtacac tgacttcttc tatacacgat
    13261 gaactcatag tttctccatc actggtcggt gatcagaacc ttaaaacagc gtagacgctt
    13321 tttgggcttt gtgagaaatc tcgcagaaaa aaccgcgagt ctataatccg acgtagaaca
    13381 gagggaaagg aggaaccttt gccgaaatga ccatccatag tgagtcggga acgatcctgt
    13441 ccccgtaaaa acataatatt tatcgtagct ttaacaactc gaaattagta taggccgcta
    13501 atttcaatca tacaacaaaa ttttgttagc agcctaagta taagtatatc gatatagatc
    13561 agtgtgattc gctatgagcg acttcggcat tgccatgatt ctggcagcca acttcttcac
    13621 aacgttgcat catgtaaccc agagcgacca gcactactgt tgataacatc acgctggtgg
    13681 tggtgagcaa tggcgtgctg atactgatca gccaggaaac taccagactt gcgaggaagc
    13741 acagacccag ttgtagagtg ttctgcaacg ctgcggcgcg accagttgcg tgtgggaagg
    13801 gacgcagcgc ctgggcgaca acaatcgggt agatcgcgcc attggcaatc gccatcacac
    13861 agaatgggat caggatttcg accagcgaca catggctaat gaagcctgca gcccaggtcg
    13921 caatgacgct gactgcaaac agcaccagca accacggtaa taactgcttg ccttgccatt
    13981 tctgcagcgc ggcgcgacag ccataaccac caatcagaaa cgcgatagtt tgcgggacat
    14041 aacttaaacc aataactgcc gggctgtagc ccatttcact aaggatgaac ggtgaaccgg
    14101 tcagccatgc aaaaaaactg gctgaacagg ccgcgtatat cagcacgttg ccgcgatagg
    14161 ttttagaacg tagcaggtcg gtaaaggtca gaccatcctg actattgtta cgggccttcg
    14221 tcgtgggttt gagccagaaa ataggcagaa tcagcaccac ggtaatggca aacagggtgg
    14281 cgaaaatcgc ctgccaggaa aaatggacca gcagccagct tcctaacaga ggagccaggg
    14341 ccggagatag acccaccagc ggcatgatgg tcgcaaaaat tcggttaact ttctgtgaag
    14401 gataataatc cgtcactaac gcttgccaga taaccgccgc ggcgcagaca cccacagcct
    14461 gtacaaaacg caataccagc agcgtagcgg cgttttctac ccacagcatc cccagactac
    14521 ctaacgcaaa aattgtcagg ccgattaata acaccggttt acgaccataa cggtcggaga
    14581 gtggccccca cagaagctgg gctgcggcaa aaccggcaag gaacagacta aggctggcac
    14641 tgacagcaga cgcaggcgtt tgcaggtcgg cctgtatggc ggcgaaagca ggcagataca
    14701 tatcggttgc cagaaaaccg agtacgctca aacccgccag ccagactaaa aatcttttcc
    14761 caggttgcat gtatattctc taaatctctc aattaaacac cgccgcaaag tgtagggagt
    14821 gaaattagtg ttgtgaaacg gtaatatttg atgactgatt tcaaaaattt tgcaggcaga
    14881 ctatgtggtc agaatattca ctcgaagttg ttgatgcggt agcgcgtaat ggtagtttta
    14941 gcgctgcggc acaggagctg catcgcgttc cttctgcggt cagctatacc gtgcgtcagc
    15001 tggaagagtg gctggcggtg ccgctctttg aacgacggca ccgtgatgtg gaactgaccg
    15061 ctgccggagc gtggtttctc aaagaagggc gctctgttgt caaaaaaatg cagatcaccc
    15121 gccagcaatg tcagcagata gcgaacggct ggcgcggtca gttagctatc gcagtggata
    15181 atattgtcag gccagaacgt acacggcaga tgatcgttga tttttatcgc cattttgacg
    15241 acgtcgaact tctggtcttt caggaagtgt ttaacggtgt ctgggatgcg ctttccgatg
    15301 ggcgcgtgga actggcgatt ggcgcaacac gcgcgattcc ggtaggcggt cgttatgcct
    15361 tccgggatat ggggatgcta agctggagtt gcgttgttgc cagccaccac ccgctggcgt
    15421 tgatggatgg cccgttcagc gatgatacgt tgcgcaactg gccgtcgttg gtgcgcgaag
    15481 acacctcgcg aacgctacct aaacgtatta cttggttgct ggataaccaa aaaagagttg
    15541 tcgtgcctga ctgggaatca tcggcaacct gtatctcggc aggattatgc atagggatgg
    15601 tgccaacaca tttcgccaaa ccgtggctta atgaggggaa gtgggtggca ttagaactgg
    15661 aaaacccctt tccggattcg gcatgttgtc tgacatggca gcaaaatgat atgtcgcctg
    15721 cgttaacctg gctgcttgag tatctaggcg atagtgagac gctgaataag gagtggctgc
    15781 gggagccgga agagactccc gcaacgggtg attaacgacg atagtcgcgg aacgggccgt
    15841 cagccacgga gcggcgttca atcaagcgcg gatgcacttc aatggactgc ggttcttcac
    15901 gtttgttgac gatacgatcc aacagcatgt tgaacgctgt ttcacccagc gaatcttttg
    15961 gctggtggat cgtggttagc gccggcgtaa aatagcgggc gttgcgcacg ttatcataac
    16021 cgatcagcga aacatcctgc gggacgcgca gacccatttc atcagcagca caaagtgcgc
    16081 ccattgccat gatatcgcca ccacagaaga cggcagtagg gcgatgcggc tgcgacagta
    16141 tttgctgcat ggcgcgataa ccagattcgg gttcaaagtc accctgaaca atccagcttt
    16201 ccggcacctt gatcatcgct tcttccatcg ccttcataaa accggcaagg cggcctgcgc
    16261 cggtgttacg ttccagcggg ccggggatga cgccgatttc gcggtgaccg cgttcaatca
    16321 gataacgccc ggccatgtag ccgccttcga acgcgttatc aatgaccgca tcggtgaagt
    16381 cagcttttgc ttcaccccag tccatcacca ccattgggat atggcgatac tcttccagca
    16441 tcgccagcaa cggctctggg tactcagaac acatcaccag cagaccatcg acgcgttttt
    16501 gcgccatcat cgacagataa gcccgctgtt tctcaagatt gttccacgca ttacccagaa
    16561 tcagggtgta acctttctgg aagcaatttt tttcaaccgc ttcaatgatc tcggcaaaat
    16621 aggccgcttc gctgctggtc gccagcaaac cgatagactt ggtgtggtta accttcaggc
    16681 tacgcgccac cgcgctaggg gagtagtgta attctttaat cgctgcccac acggcgttgc
    16741 gcgtttcttc agcgacgaaa cgtgttttgt tgattacgtg tgacacagtt gtagtggaaa
    16801 cgtttgctcg tttcgctaca tcttttattg ttgccattcc atttcactcc agaccctaac
    16861 ttcagctcct gcaaaatcgc aaggtaaacg tttgccttta cacaccttac gcacaacaaa
    16921 agtgttgcgg tacgccggaa aaacgacacg gagcgtcagg aaggggtcaa tggccagtac
    16981 gcaaatagtg taagcgtgga attttggccg atcttgacga aaaggggaag tggtaaaaac
    17041 acttatcatt ataaatgacg ggagattttt tcatcacagt gtgtaaaaat gcgtaatatc
    17101 actatttgtg tggtaataaa aaaggagaaa acttgatgag caccgacctt aaattttcac
    17161 tggtaacaac gattatcgtc ctcggtttga tcgtggccgt gggtttgact gccgcgctgc
    17221 actgatttcc tctcgaggga gcgcgttctc cctcgcatct ccttcctgtt tgttagcaaa
    17281 atggcaaaag tgttttctct ttttgttatt ttcatttttt tgtgattgct gtcatgcttt
    17341 cgccacttta atgaatatcg cgtaatgacg cgaagaggtg gagataaggc atgaaaatta
    17401 actatccgtt gctggcgctg gcgattggcg cgtttggtat cgggacaacg gagttctcgc
    17461 caatgggctt gttgcccgtc attgcgcgcg gtgtggatgt ctcgattccc gctgccggaa
    17521 tgttaatcag tgcctatgca gttggcgtaa tggttggcgc gccgctgatg acgcttctac
    17581 tttctcatcg tgcccgccgc agtgcgttga ttttcctgat ggcaattttc acgctcggca
    17641 acgtactttc cgccatcgcg ccggattata tgaccctgat gctttcacgc attttgacca
    17701 gcctgaatca cggagcattt tttggtttgg gttcagtcgt ggccgcaagc gtggtgccaa
    17761 aacataaaca ggccagcgca gttgccacta tgtttatggg gttaaccctg gcaaatatcg
    17821 gtggcgtgcc ggcggcgacc tagttgggtg aaaccatcgg ctggcggatg tcatttctgg
    17881 caacggcggg gctgggagtg atttcaatgg taagtctgtt cttctcatta cctaaaggtg
    17941 gtgcaggggc acgacctgaa gtgaaaaaag agctggcggt attaatgcgt ccgcaggtgc
    18001 tgtctgcatt gctgacgacg gtactgggag ctggtgcaat gtttactctc tacacctata
    18061 tctctccggt actacaaagt attacccacg caacaccagt gttcgtcacg gcaatgctgg
    18121 tgctgattgg tgtcggattc tctatcggta actatctcgg cggcaaactg gcagatcgtt
    18181 cagttaacgg cacgttgaaa ggctttttgt tgttgttgat ggtgattatg ctggcaatcc
    18241 cgttcctggc ccgcaatgag ttcggcgcag ctattagcat ggtggtgtgg ggcgcagcaa
    18301 cctttgcggt cgtaccgccg ttacagatgc gcgtgatgcg tgtcgccagt gaagcgccgg
    18361 gtctgtcttc atcagtcaat attggtgcct ttaatcttgg aaatgcgctg ggagcagctg
    18421 ctggtggtgc ggtaatttcc gctgggctgg gatacagctt tgtgccggtg atgggagcga
    18481 ttgtcgcggg actggcatta ttgctggtgt ttatgtcagc cagaaaacaa cctgaaacag
    18541 tttgcgttgc taacagctaa ttaaacaaaa acgcagaagg ataaccttct gcgttacagt
    18601 tgctgacaac gtgcgattta tcttgccgaa tgcgctgtta acgcctcatc cgcgttggca
    18661 tggtaagcga agcgcatcag gcaatgctgc atttgccatc agttattatg cagcgagatt
    18721 tttcgctacg aattcccagt tcaccagcgc ccagaagtgc tccagatagc caggacgtgc
    18781 attgcgatag tcgatgtaat aagcgtgttc ccagacatca acggtcagca gcggagtcgc
    18841 atcggtggtc agcggagtac ccgcgttaga ggttgaaacg atagccagtt taccatcgct
    18901 gtttttcacc agccaggtcc agccagaacc aaagtttttg atcgctgcat cagtaaactg
    18961 cgctttgaaa tcggcaaagc tgccaaaaga tgcggcgata gcttcagcga cttttccagt
    19021 cggttcgcca ccggcgttcg gtgccaggca gttccagtag aaagtatggt tccagacctg
    19081 agctgcgttg ttgaatacgc caccttcaga gctgcgaata atctcttcca gtgatttacc
    19141 ttcaaacgcg gtacctttaa tcaggttgtt caggttagtg acataagtct gatgatgctt
    19201 gccgtagtga tactcgatag tttccgcaga aatgtgcggt gccagagcat ctttagcata
    19261 tggtagtgca ggtaattcga atgacattgc tactctcctt tattattaat ttgcatacgt
    19321 acaatagcct tattgtgcgt atggataggg tagcaaatta aaaggtgaga caaaagatga
    19381 acttaccctg ttgccgtaac aacagggtaa agttttagcg aagtgttttt ggggtcatta
    19441 cccgacgagc gccaacatag tggcgctgcc agtagtcttc actgagagaa gtgatttgaa
    19501 tttcctgacc tgtgcgcggt gactgaataa atttgccgtt gccgacatac acgccgacat
    19561 gatcggctgt gccgcgtccc tgagtacgga aaaagaccag gtcgccgttt ttcagttcac
    19621 tacgttcgat tggcgctgca tcacgcaggt gatacatttc attcgccgta cgcggaatac
    19681 gaattttcac caaatcttta taagcgtaat aaaccaggcc gctgcaatca aaaccggtac
    19741 gcggtgagct gccaccccaa cgatatggct taccaatttg ctgcatcagt ttattcattg
    19801 ccactttcgt agctttttgc accttcgctt tgtgcgcgtc ggcaagagtt cctgacgcgg
    19861 catttttgac tttcacacaa tgcgatttat agcccttacg tttggtacac ttttcggtaa
    19921 agctggctga ggccgtttta ttgacggcgt gtttgctgcg agttttggcg tttttagaag
    19981 cggtaatgga agatttttta acggaagaag cggttttgct tttggtcgta cttttactgg
    20041 cggctttttt cgctgttttc tgggtctttt tggtggttgc cgtgcttttc tttttatctg
    20101 cttttttggt gatatgagtg gtagcagaac tctccctggc ttgctttgaa gcatgggcca
    20161 taggcgttaa aggcagggtg gtaaaaagta aagcacagag cgtgatcgaa atacggttta
    20221 tccgcgccac tgagcgtccc tctgttaagg caggtctatc atgcctgcgt ctgatttaca
    20281 aaacttgatg attctaatgc atagaagaag agaaagttaa tagcttttat tgttaaaaaa
    20341 tacgtttcgt tggcgagcgc aaaaaaatgc agcagtgaaa gctaaaaatg gttataaata
    20401 cgccagtttg ccggcatcag agaagaaacg aatatttgaa atgcaacaaa tagagcaaag
    20461 ggataaaata gcaaaagcgt gatgaaaatg ttattttccg atgccgcact gacccgctac
    20521 aatgtcagat tactgccgta aagaagtttg aggaagcaag acaatgagca ccactatcga
    20581 aaaaatccaa cgccagattg ctgaaaaccc gatcctgctg tacatgaaag gttcaccgaa
    20641 actgccgagc tgcggtttct ctgcccaggc agtccaggcg cttgccgcat gtggcgaacg
    20701 ttttgcctat gttgatattc tgcagaaccc ggacattcgt gcggaactgc cgaaatatgc
    20761 taactggccg accttcccgc aactgtgggt tgacggcgag ctggtaggcg gttgtgatat
    20821 cgtgatcgaa atgtatcagc gtggcgaact gcagcagctg atcaaagaaa ctgccgctaa
    20881 atacaagtct gaagagccgg acgcggaata atccgtcctg caatactgac ggatgcgaac
    20941 aggcatccgt cagtcctttc tctatcccca atccagccct tgtggcgaac ttgaaaatcc
    21001 cgcctcacgc agcagcgtaa acatcggcgt ttgccggacc ggtagatcat tcacttctgt
    21061 tagcgtaaag cgcagccgtg gttcgcgacg cagtgcggta gtcagcgcgt ggaaaacctc
    21121 tggggcgagt aattcctctt tttcctgcca gaccagcatt tttttgccac cttgcgccag
    21181 ataaagtaac aatttgccgc cagaaacgac caccagcgca cccgcccgac gcgttggaac
    21241 cagcgttgcc ggatgtgcag gccagggcag aagatttccc cacacattag ccggatcgtt
    21301 ggcagagagc tccactggtg tatagtggcg cgtttgcgtg gcttgtgtcg ccagatcgcg
    21361 caatcggtca atagtcagac gttcagcgaa ttgcgcgcca cccagacctt ctacaaaacg
    21421 acctcgcata attcgcccgg agtcttccat acttcgacaa agtgtttgca tcgatggaaa
    21481 cccgccaggg atattttcgg ctatcaccgc ctgacgactg atgatgccgt agcggtcgag
    21541 catattttcc gccagcgcca gcatcctttc ggtatcgttt agtggctcca cctgcaataa
    21601 cgaccagcgt ccagccagat ttggtgtgtt gtaagatacc cgcggcgaga cgggttgtgc
    21661 atagacagga cgcccacgac gagcccggtg actgcggcga gttgaggtgc gtgcgttgga
    21721 actgctgcgg gtgagggcgc gtaacggtgc ccaaatatcg ctggtaatga cgccttgcca
    21781 gaccagcgcc cataacgcct cttgcagtgc cgaggaatca accgattcgc cgattttatc
    21841 gtgtatccgt tggctgattt gttgtgcaaa ccaggctcct ccgtcagcca gaacagcgat
    21901 tatcgcttgc tgcagcgccg aacgattcgc ctgatccgct tcggcgggag tgaacgattc
    21961 tgcggcatat tcctgtagat gcagtgccac caggccgtca tcttcaccca gctttttttg
    22021 tcccgaccag ataaccgctc cggttgccag taattcatcg agcatttctg gcgaatagtc
    22081 gcgtacgcgg gcaggcagga tctggctttc ccagagtgag gcgggtaaac cgactccggc
    22141 aagctgttcg atcacccgca tcacgccatc tacgccctca taaacgcctg gcgatgttga
    22201 ggcaaagagc gccgggctac catcggtggc gggtaatacg ccctgacgtt ccagcagcaa
    22261 tcgcgcatag gtcgtggctg caacgggacg cgtcgcttct ctggcggctt gcagcgagcg
    22321 caaacgcaga cgacgaaata cttcatcgct cacccagatg tcttgttgca gattcatcac
    22381 cagaccctgt tcacgcagtt gctgaagctg ctcttcgaca atggcaatac cgagactaaa
    22441 ctcatgagcc agttgttcag ccgtgaccag agcatgagcc cggagatagc gcagaaagag
    22501 atcgcgaagc gggtaactga ctctatgtaa ataaatctct ggcaatgact ctggtagtcg
    22561 tacgccgagg gcatcacgca gcctggcggc atcatccata caggcaagac gctcctgtcc
    22621 gctaatcatc gctgggaaga ttcgttttac tgcaagaaga ttttccagat acgacgcaac
    22681 ctcttcactg ctgcctgtat gccgttgcgc caggtcttca acggtcattg gccccagttc
    22741 gcgcagcagg tcgaacaatc cttcttcacc tttcgctctt ctgccaggag ccagtcgttg
    22801 caactcttct tccacctggc gaatgacctg cgggtcgagt aattcccccg gatcgacctg
    22861 tccgagtaga ttgcgcagta actcgctgtc cagcgacagt acggatgccc ggcgctctgc
    22921 cagcggggcg tcgctctggt acataaattc cgcgacatag ccgaacaata aacttgtggc
    22981 gaaaggcgag ggcgtagtgg tcgttacatc ggatatttga atttcgccac cgttcaggcg
    23041 acgcatcaaa cgttccagtg cgggaagatc ataaacatct tgcagacatt cgcgtagggt
    23101 ttcgagaatg accggaaaat ccggatatcc ctgagcgatt tccagcaact gactggcgcg
    23161 cagccgttgt tgccaaagcg gggtgcgatg gcccggagtg cgccccggca ttaataatgc
    23221 ccgcgcggcg cattcgcgaa aacgggcggc gaaaagtgcc gagctgccta ccgcctcgcg
    23281 gacaatttgc agcaactttt ctggttcaaa caaaaaaatc gcggcgtcgg gcaatttacc
    23341 atcagtgtca ggaatgcgcg caacaatgcc gtcatcactg gcgaccacag acgcgtcagc
    23401 gccccatagg gcatgtattc gcccggcaat cgccagcgcc cagggttcat gcacccgtct
    23461 tccataggga gagtgcaaaa taatacgcca gtcgccaatt tcatcacggc accgttcgag
    23521 gaccagatgg cgactccccg gaacgatgcc cgtcgcgttg cgttgctcgt caatcaaccc
    23581 ctgaatattg gcgatcgtat tttcttctgc cagccacggg ggaatagtcc cggaaaagaa
    23641 cgcgccatcc gccagcaaat gaagaaaatc gccgatcatc tcgcctaatt cagccggacg
    23701 tccgttacct tcaccacgcc agaaggggag ccgggcagaa cgacccggag caggagtcac
    23761 aatcacctga tcgcgggtga tttgctggat ccgccatgag gtagcgccga gagtgataat
    23821 gtcgttcacc cgcgactcat ataccatctc ctcatccagt tcacccaccc gccgcgaact
    23881 ggcctgttct tcaccttcgg gtaataacac gctatacatg ccacgatccg gaatggtgcc
    23941 gccgctggta accgccaaca attgagcgcc aggtcgggcg gtcaatatcc cggtctccct
    24001 gttccagacc agtttggggc gaaaagcaga aaaatcgcca gagggatagc gcccggaaag
    24061 catatccagc gtggcgtcaa aaacacgtct tggcagatct ttccacggtg cggcacggtg
    24121 tacgcgggag taccattcgt ctacctgtaa tgcctccatc gccgcggcgg caacggtttg
    24181 ctgcgcaagg acgtcgagtg gattatgggc ggtgtcaggt tttccagcct gcctgcgaac
    24241 atacactcta caatgactgc ggaatcgact aagtcacgcc gggtacgagg gaaaaacagc
    24301 cctttagata caccgccaac ctgatgtccg gcgcgaccaa tgcgttgtaa cccactggca
    24361 acagaaagcg gcgttgccac ctgaatcacc agatccactg cgcccatatc aatccccagt
    24421 tcgagactgg aggttgcgac cacgcagcgt aactcacccg atttcagcgc ctgctcggtg
    24481 attgctcgtt gttctttaga gacggagccg tggtgtgagc gggcaataaa aacgtcgcta
    24541 ctttgtacac ggttagaggt tgcgccggag gtcgactcga aatgggccgc atcaacggcg
    24601 atagacgggg aacgctgtaa gcgtgcggcg taaagctcat ttaatcgtgc cgtcagtttt
    24661 tccgccagcc cacgtgaatt agtaaagaca atggtcgagc gatggcgcaa cacttcatca
    24721 aggatacccg tttcaatata tggccagatg gagccttccc ggccggcatg gctgtcttcg
    24781 ccggtgctgc tggcgaccga tgagacatca tccatattgg cgaccggtac gacaattcgt
    24841 atctgcggat ggcgcattgc gggcgggttg actaccgtaa ccgggcgatc gccaccaaga
    24901 aatgctgcca catcgctggc tgagcgcaca gtggcagaaa ggccaattcg ctgtgctgag
    24961 gtgtggagca gcgcatcgag ccgctccaga cttaacgcca gatgcgcacc acgtttactg
    25021 cccgctaccg cgtggacttc atcaataatt accgtttcga cgccgcgtag cgtttcgcgc
    25081 gcgcgggagg tcagcatcag atagagtgat tcgggtgtgg tgatcagaat atccggcgga
    25141 ttacgggtga gtttgctgcg ttcctgtgca ggcgtatcgc cagtacggat ccctacgcga
    25201 agattgactt ccgtttcgcc gcgccgccgc cgttcatcgg caataccctt caacgggagc
    25261 tgcaagttgc gctgaacgtc ggtgcccaga gcttttatcg gtgaaatata gaggatgcgt
    25321 gaggttttac gcttatgcgc ctcgcgggta tcttcgccgc cttcgcggaa gagccgatcg
    25381 agggcgtaga gaaatgctgc cagcgttttc ccggagccgg tcggtgcaat caccagcgca
    25441 tgttcgcttc gcgccgccac atgccaggtt tgcgactgga cagcggtcgg ctgtttaaag
    25501 gcgcgaagaa accagtcgcg ggtcgccggt gaaaacacgt ccggcaggag cgatgaaggg
    25561 tctggattat ctgccattaa cgctattccc cgtgaatatc atgcaggatg cgtggaatag
    25621 cctcactata gatgcctgaa tcattgcatg tcatcaggca tcgactcgat tacacctctt
    25681 cggcggcagg tagcggccag cctcccagac gtttccagcg gttgacgatt tcacaaaaca
    25741 gcacagcagt gcgttcggtg tcgtacagcg cggagtgcgc ctgggtgctg tcgaagtcca
    25801 tgccagcggt ctggcaagcc tttgacaata cggtttgtcc gagtgccagc ccggccagtg
    25861 cagcagtgtc aaaagtggcg aaagggtgga acgggttacg tttcagtgag gcgcgttctg
    25921 cggcggccat cataaagctg tgatcaaaat tggcattgtg cgccaccata atggcgcggt
    25981 tacagccgct cgctttaata cctttacgta caactttaaa aatttcgtgc agcgcctcgt
    26041 attcgctgac cgcgccgcga tcgggatcgt tcgggtcaat gccgttgaag gcgagggctt
    26101 ctggttgcaa atttgcgccg acaaatggtt cgacgtggaa atgtaatgtg gtgtccggca
    26161 tcagccagcc ttgttcatcc attttcaggg tgatggcggc aatctcaagc agcgcatcgg
    26221 ttttggcgtt aaatccggct gtttcaacat cgatcacaac aggataaaaa ccacgaaaac
    26281 ggtcgcacag accggtaagt tgagcgttat cggacatctg attctcttaa tacggataaa
    26341 ttgcagcgcg cattatgaca aatattactt gtcgatgcag taaagatgcg ggcgcgatga
    26401 gttcacgccc ggcaggagat tagttgccca gaccgcgacc ggcgtctttc tcttcgatta
    26461 actcaatttt gtaaccgtcc ggatcttcca caaacgcgat aaccgtagta ccgcctttta
    26521 ccggacccgc ttcacgggtc acgttacccc cgttttgacg gattttttcg cacgcttcag
    26581 cggcgttatc tacgctaagc gcgatgtgac cataagcagt gccgagttcg tatttatcca
    26641 cgccccagtt gtaggtcagt tcaatcaccg cttcttcggt ttccgggccg tagccaacaa
    26701 acgccagtga gtatttgtat tccgggtttt cgctggtacg cagcagtttc atgcccagca
    26761 ctttggtata aaaatcgatg gagcgttgca gatcgccaac gcgcagcatg gtatgaagaa
    26821 gacgcatctt tttatcctca aaatggctaa cagattcaaa atgttgtttt agtatagcgg
    26881 cggctttacg ccgctcgcaa tgttggatta caacgtcggg taatcggtat agccttccgc
    26941 gccgccaccg tagaaacttt cggcacgctg tgggttaagc tcagctttgc gctgcaagcg
    27001 ggcgaccaga tccgggttcg caatccagtc acgaccaaat gccaccgcat caattaaccc
    27061 tttgccgatc agcgtttcag ctttttctac tgtgtatgca cctgcgccga taatcggacc
    27121 gtggaaacgg gcgcgtactt tttcgcggaa cgcatcagta tacggttcac cccccgccca
    27181 atctggttct gacatatgca gataagcaat gccgcgttta cccagttgtt caatcagata
    27241 cagtgcatcg gcttcttcat tcgggccgtt atccgtgttc tggaaagtac cgattggcga
    27301 aacgcgaatg ccaatgcgat cggcacccca ttcttcaatc ccggcatcga ccacttccag
    27361 taccagacgt gcgcgatttt ccacgctgcc gccgtactga tcggtacgat ggtttgaaga
    27421 aggagaaagg aactgatgca gcaaataacc gtgagcagag tggagctcta ccagatcaaa
    27481 accggcttca cgcgcgttag caatggcctg acggaaatca ttgacgatac ctggaatctc
    27541 ttccagttca agcgcacgcg gcatggatgt ttcaacacgg atcgcctgac cattttcatc
    27601 gcgcagagaa gtacgtgttc ccgcgctaag tgctgaaggc gctaccggtg cctgaccgcc
    27661 aggttgcagg ctggcgtgag aaatgcgtcc ggtgtgccac agctgcacgg ccatatgacc
    27721 attttcagca tgaacgccag cggtgatttt tttccatgcg gcaatttgct ccggactatg
    27781 gatgccaggc gcacctgcat atccttttgc ctgggcagaa atttgcgtgg cttcactaat
    27841 aatcaaaccg gcactggcac gttggcgata gtattccgcc atcaacgggg taggaatgtc
    27901 acccggttca atactgcgca gacgcgtcag cggtgccata aaaatacggt ttgccgccgt
    27961 gatcgcgccc actttcagtg gggaatacag tttttcagat gacataaagg ctcccggtcg
    28021 tctggtgata aaaaatgcct gctaaacggc aggcgtcgca ataatgtttt ttacatgggc
    28081 cagcgcgttt tccagtggct cgaaactgcg cgaaattttg gcctgcagat tcgcacccag
    28141 ccatagtgcg taaagcactt gtgcctgttg tagcggttcg ccacaaaagg ttaaacaatg
    28201 gttctcacgg ccattttcca gcgcctgaga gagcagggcg atcacgccgc gagcaccttt
    28261 atccatcgcg ctgcgcatat cttctgacag atcgcacact tcggcagaga gttttactgt
    28321 caggcaacca ctgatggttc catgttggca aaactggttc agtgtttgct ggtaataagc
    28381 cagtatgcgg tcgcggtagt taccttcgcc ggattgcagc aactcagtca gtcgctggtg
    28441 atatgcggcg taatgacgct caagcatggc aacgccaaac gcttctttag agcgaaagta
    28501 gtgatagaag gaccctttcg gcacttcagc ggtttttagt aattcgctta gccccatccc
    28561 ggtgaatcca cgttgcaggc aaagttgctc gcccgtcgcc aggagatgtt cgcgagtatc
    28621 atgttcggtg tgtttgttca tgcgttggag tgtagtagac cagtcggtct agtgcaagca
    28681 aagttgctac tttggacatt aacagcttca ataatggtaa taatcttatc tgaggagggc
    28741 gaagtggcgg agcaattaga gttctttccc gtccagagcc cgtgccgggg aatttgccag
    28801 tctgacgaac gcgggttttg tcgcggctgt tttcgtagtc gtgatgagcg ttttaactgg
    28861 aataaaatga gcgatggcga aaaacaagag gtgctgcgcc tttgtcggca gcgattaatg
    28921 cgcaaattac gcgcaaataa gccagcgtca tcagacgaac cggagcaacc atcactcttt
    28981 tgaacctaat cttgcgtata ctcctctcat atctgataag aggaagcgat tatggttcag
    29041 cgtattacta ttgcgccgca aggcccggag ttttcccgtt ttgtgatggg ctactggcga
    29101 ttgatggact ggaatatgtc cgcccgccag ctggtcagtt ttattgaaga gcatctggat
    29161 ctcggcgtga ccaccgtgga ccatgctgat atttatggtg gctatcagtg cgaagcggcg
    29221 tttggcgagg cactgaaact ggcacctcac ctgcgtgaac ggatggaaat cgtcagtaaa
    29281 tgcggtatcg cgacgaccgc gcgtgaagaa aacgtcattg gtcattacat cactgaccgc
    29341 gatcacatca ttaagagcgc cgaacagtcg ctaattaatc tcgcgaccga tcatctggat
    29401 ctgctgttaa tccaccgacc agacccgtta atggatgccg atgaagtggc ggacgcgttc
    29461 aaacatctgc atcagagcgg caaagtgcgt cattttggcg tatcgaactt tacgcctgcg
    29521 caatttgccc tgttgcaatc acgtctgccg tttacccttg ccactaatca ggtggaaata
    29581 tccccggtgc atcagccgtt actgctggat ggcacgctcg accaactaca acaactgcgt
    29641 gttcgtccga tggcgtggtc ctgccttggt ggtggtcgtc tgtttaatga tgattatttc
    29701 cagccgctgc gtgatgaact ggctgtggtg gcagaggagt taaacgcggg ctcgattgaa
    29761 caggtggttt acgcctgggt attacgttta ccatcgcagc cgctgccaat tatcggttca
    29821 ggtaaaattg agcgcgtacg ggcagctgtc gaagcagaaa cactgaaaat gacccgtcaa
    29881 caatggtttc gtatccgtaa agcggcactg gggtacgacg taccgtaagc acttttagga
    29941 atagccgccg ttcaaaaatg tgtcactggt ttacacttat tcaggaatgc acaatgaacg
    30001 gaggtcctat gaaacgtttt agtctggcta ttctggcgct ggttgttgca accggcgcac
    30061 aagctgccag tgaaaaagtc gagatgaacc tcgtcacgtc gcaaggggta gggcagtcaa
    30121 ttggtagcgt caccattact gaaaccgata aaggtctgga gttttcgccc gatctgaaag
    30181 cattatcccc cggtgaacat ggcttccata ttcatgccaa aggaagctgc cagccagcca
    30241 ccaaagatgg caaagccagc gccgcggaat ccgcaggcgg gcatcttgat ccacaaaata
    30301 ccggtaaaca tgaagggcca gaaggtgccg ggcatttagg cgatctgcct gcactggtcg
    30361 tcaataatga cggcaaagct accgatgccg tcatcgcgcc tcgtctgaaa tcactggatg
    30421 aaatcaaaga caaagcgctg atggtccacg ttggcggcga taatatgtcc gatcaaccta
    30481 aaccgctggg cggtggcggt gaacgctatg cctgtggtgt aattaagtaa ttaagaggcc
    30541 agcgtacctt gcggtggtgc ttgctcaagt tgcgaaagcg agcagtacag ccgccagaca
    30601 attgccgcca gctcacgggc ggcaggttga tgatgacggg caaggctgtc gcaaatccgc
    30661 tgtaattctt caagtgtggc cgccagtgat ttttgctgaa cgccacgctc actcatcact
    30721 ccccgcaaca gtgaaataca gttatcccgt actcgcgata acggatcgga acgcgattcc
    30781 cagtcgcgca attgccagac aacatgagaa cagttcagca gcactacacc ccagcgtaat
    30841 aaccaacggc gagccagcgc atcctggctg ttactcaact gactgacgtg atgataagtg
    30901 agcgattcaa attcgctttc actcagtgtt ggatggcggc ttagctgatc gacaaaatcc
    30961 cggcgcagcg cgcgaatatg gcggcggctt ttacgagcat ccgatcctgg acgcagaatg
    31021 gcgaacgcta gccaagccag cgcaacgcca acgatttttg ccagattatc gttaagaaaa
    31081 tcagcaaaat catacaccgg gggattagtg acagcgataa acgaacccat aaaaacaatc
    31141 agttgccccc acagtgcggc aaatttgggc atctgcaatt taagaagctg cattgtcgcc
    31201 agcagtggaa agagaaacag taaaaattgc cacagatcgc taatctggac catcaggcca
    31261 aatttgacca caaagctgaa tagcgaaagt aacaccagcg tgcgcatcag cagtgacaac
    31321 gacttaaacg gtgctgcgac ggcggagtag agtacgcagc taattgctgc cagcgttaat
    31381 gcattggcac cggcatccca ttgcgatgca atactccatg cgccaatcat catcaacgta
    31441 caaaatgtac gcagcccgct ccacatagct tcggcgttgt cggtatgacg agccagagcg
    31501 gatgtccgtg cgagtctggc atgatcatct acaccgcttt gcagacgatg taattcctga
    31561 ctactttgca gataaaggcg gcaaaaataa cgtagtcgct gccagaaggc gacgtgccgg
    31621 tagtcggcga cgttggtcgg gcgtagcggg gcgataatac gtgcgacggt gtaagcatct
    31681 gtttgcgaac tggcgagcgc cgtcagcaat tgttcgagaa tttctcgcgt ggcacctggc
    31741 ggtgagggcc agttgagcaa catacgtcgc aggctggaga tgacactggt catacgtaat
    31801 tgctggtgga gtagcgcatt aaggcgcgcg ttttgctggc gaaaacgata gtggctccag
    31861 aaagcctgga tacgcagcaa attcatggtc agtatctgcc caatcacccc ctcatgtgct
    31921 gcacgaatgg catcgtttgt ttcaggctgc cagagtaaac tggcatgttc cagtaatcgg
    31981 gcgtgcatgt ttttcaatgc ggttaaaagg gctgtagcat cggaactgct aggcaggatc
    32041 atcatcatca ttccgccgca caaaataccg actatcacct cgcaaacgcg cgcctgagcg
    32101 atatcccaca gctggctggc ctcagtaata ttaaccatcg gaaaggcgat gatggcagcc
    32161 gtgtagcccg ccagttgaaa tgcatacgcg acgttattcg tgaagtgcgc acaggcccag
    32221 gtacaaaagc caagccacgc cgacatgctc aatagaaaaa accacggctc attgagcgta
    32281 tgcccggcaa gaagcagtgc cgcaatggct cccagtaaac tgccagcgat gcgtccgagg
    32341 cttttgctga tgacaccgcc aacggtggga aagctaacca ctgcagccga ggtcatcgcc
    32401 caatagggtt catccagatt caaataatag gcaaccgtca gcgccagaca catggcaatg
    32461 gtattgcgta acgcataacg ccattgcgcc agcgtggcct tgaaccaggg caaattgcgc
    32521 aaggaccatg acgatgcgtt cattattgac ctacggcaat gctacaggtg gtgcccgaca
    32581 ccagcgtgac atcgcctgga actttatcca gcgcaaagcg gacgggaacg cgttgggcga
    32641 gacgaaccca ggggacgtta ggtttcacat ccggaataag gcttgagtca ctttcaacgc
    32701 tttgatcata aatcgcccga ccgatactgg aaacgtgacc ctgtaacgtt ttgttatcac
    32761 tatagagggt aatttgtgct ggtgcacctt cgcggatatg gcgcaacttg gtttcttcaa
    32821 aataaccaat gacgtaaaac gagtggctgt cgacaagggc aaacagcggt ttcccggtgt
    32881 cagcgtaatc gccgatgcgg gtagtgaggt tagtcaccca accactcacc ggggcgcgaa
    32941 tttccgtttg cgccagttgc cattgtgtct gtttcagcgt ggcctgtgcg gcattaacac
    33001 tggcctgcat cgctttaaca ttgaggttag cggtatcaag ttcttcggca gaaataaaat
    33061 tttgtgagag atgacgacga cgattcgctt cgttattggc ttttgcgagg tcagattgcg
    33121 ctttcgccag ctgagcctgt gcgttcagct cggcgatctg aaagggcgtt ttatcgatcg
    33181 tgagcagaag atcccccgcg ttaaccagtt ggttgtcttt tatattcagc tcaacaatac
    33241 gtcccgacac ctgtggggtg atagaaacct tttctgcgcg tattttcccg tcgcgtgtcc
    33301 acggagattg catgtaataa ttccacagcc accatccggc aagaaccgca actatcgcta
    33361 caatgattgt tgagaaatac ttaattgttt taattgacat agttaccacg caatcagtat
    33421 tgccagagca aggcaaacgc aaatcgcaaa cagcgataaa tccattaaca agggatgcca
    33481 gatgtcaccg gcgtagatcc agccacgaag caggcggtgt acgacaagcc agatgacgaa
    33541 tccaaacgcg aaggctttga aaaacggagg aaaatagacg gacgcaccga acaccagatc
    33601 ttgtaagggc aatcctgttg cattgagcat aaacttcaca aagtagattc ctttacgacc
    33661 gaatatattg cgttagatta ataaatattc tttaagtgcg aaaaatttac gcgcaatttc
    33721 tgaaaaatat gtctgacggt aaccaaatgc agcaatacat ttgttttagc aatacaattg
    33781 ctgcacacta ttctaaaagc cgcataatat cttagcaagc taattataag gagatgaaat
    33841 tggaatcgcc actaggttct gatctggcac ggttggtgcg catatggcgt gctctgatag
    33901 accatcgcct gaaaccgctg gagttaacac aaacccattg ggttacgtta cacaatatcc
    33961 atcagttacc tccagaccag tcgcaaattc aactggcaaa agcgattggc atcgagcagc
    34021 catcactggt ccgtactctg gaccaactgg aagaaaaagg gttaatttcg cgtcaaactt
    34081 gtgccagcga tcgtcgggct aaacgtatta aactgacgga aaaggcagag ccactgatca
    34141 gcgaaatgga agctgttatt aacaaaaccc gcgcggaaat attacatggc atctccgcag
    34201 aggaactgga gcaactgatt acgctcatcg caaaacttga gcataatatc attgagttac
    34261 aggccaaagg gtgaaatgaa gggggcgtgt ggtcagtaac ctgaccacac gcaaacttat
    34321 ttagcgcgga gaaacggtca cctgactgcc attgctggcc agtacgacac gttggcccgg
    34381 agagaaacga gtgttgcctt gtttctgtac caccatgatg gtattaccat cgtctttacg
    34441 aatttccagc tcgacaccct gcgttttgtt cattgcactc tgtacgccct gaccagctac
    34501 gccacctgca acagcgcctg ctgcagtagc cagagaacgc ccggttccgc caccaacagt
    34561 attccccagg aaaccaccaa gaacagcacc gccaattgca ccgataacgt tggaatcatc
    34621 accgccctga atctgtaccg gacgtacgtt aacgatggtg ccatagctga cattctgtac
    34681 ttgtttcgct tcagaagcgg tataaacatc ccctgacagg gtgtcgttat taacacaacc
    34741 gacaagagac agacctacca ttgaaacaac caatacgcgt ttaatcattg aaaaatctcc
    34801 tgttcaccat gaaacgccac gcaagcatcc ctcatggtca aagtatatgg catatgtgaa
    34861 tccatggtta catgttctac caaatatgag caaatcataa tgaaagtagg atcaaccaat
    34921 tataaacaaa acattcatga tgattattga tttgaagata atgttttact accaacggta
    34981 aaaatcggtc attccgcatg gtattcacga aaagtgtatg tttgagtggt gcgcaaacgt
    35041 cagattgaaa aaggatgaac tatgaaatcg ggccgcttta ttggcgttat gtcaggcacc
    35101 agccttgatg gtgttgatgt tgtgttggcg acaatagatg aacaccgggt cgcacagctg
    35161 gcaagcttga gctggccgat cccggtgtct ctgaaacagg ctgtactgga tatttgccag
    35221 ggccagcagc ttacactttc gcagtttgga cagcttgata ctcaactcgg gcgacttttt
    35281 gctgatgcgg tcaatgcctt gcttaaggaa caaaacctgc aggcgagaga tatagttgcg
    35341 atcggctgtc acggtcaaac cgtctggcat gaaccgacgg gcgtggaacc acacacttta
    35401 cagattggcg ataacaatca aattgtggca cgcaccggaa ttacggttgt cggtgatttt
    35461 cgccgtcgcg atattgcctt gggaggacaa ggcgcaccgc tggtacctgc gttccatcat
    35521 gccttgctgg ctcacccaac cgagcgacga atggtgctca atattggcgg catcgccaat
    35581 ctgtcactgc tcattcctgg gcagccggtt ggcggctacg ataccggtcc tggtaacatg
    35641 ctgatggatg cctggatctg gcgtcaggcc ggtaaacctt acgataaaga tgccgagtgg
    35701 gcacgggcgg gtaaagttat tctcccactg ctgcaaaata tgctcagcga cccatatttc
    35761 tcgcaacctg caccgaaaag caccggacgc gaatacttta actacggctg gctggagcgc
    35821 catttacgcc attttccggg tgttgatccc cgtgatgttc aggccacact ggcagaactc
    35881 accgccgtga ccatttctga acaagttttg ttgagcggtg gctgcgaacg attgatggta
    35941 tgtggtggag gtagtcgtaa tctgctactc atggcgcgtc tggcggcatt actgccaggc
    36001 acagaagtca ccaccaccga tgccgttggc attagtggcg atgacatgga agcattggct
    36061 ttcgcctggc ttgcctggcg gacgctggcg ggattaccag gaaatctgcc ttccgtcact
    36121 ggcgcaagcc aggagacggt actgggggct attttccctg ctaacccgtg atatcagagt
    36181 taactgaatt tttcttaccg cgtctgccgt tacacttgga acattaggga gggcgtattg
    36241 ccctccagac caggaaagtc ttcgggagat gtctatgaaa aaactgttac tgatctgttt
    36301 gccggtgctt ctcaccggct gtagcgcgtt caatcaactg gttgagcaca tgcaaaccgc
    36361 tacgctggaa taccagtgtg atgaaaaacc gttgacggtc aaactgaata atccgcgcca
    36421 ggaagtcagt tttgtttacg ataatcaact actgcatctc aaacagggca tttcagcgtc
    36481 tggtgcacgt tacactgacg gaatctatgt tttctggtcg aaaggcgatg aagcgactgt
    36541 ctataaacgc gaccgcatcg tcttgaataa ctgtcagtta caaaatccac agcgttgaga
    36601 tttttccagg ggcggcgcac aatagcgtca cccactgaca atccgtaaag aaaaccatgt
    36661 ctgataacga cgaattgcag caaatcgcgc atctgcgccg tgaatacacc aaaggcgggt
    36721 tacgccgtcg cgatcttccc gccgatccat taaccctttt tgaacgttgg ctctctcagg
    36781 cgtgtgaagc caaactggca gaccctaccg cgatggtggt cgctaccgtg gatgaacatg
    36841 ctcagcctta tcaacgcatc gttttactca aacattacga cgaaaaaggc atggtgtttt
    36901 acaccaacct cggtagccgt aaagcacatc aaattgaaaa taatccgcgc gttagcctgc
    36961 tgttcccgtg gcataccctt gaacgccagg tgatggtgat cggtaaagca gaacgacttt
    37021 cgactctcga agtgatgaaa tattttcata gccgcccgcg tgatagccag attggtgcat
    37081 gggtttcgaa gcagtccagt cgcatttctg cccgcggtat ccttgaaagt aaattcctgg
    37141 agctgaagca gaagtttcaa cagggcgaag tgccattgcc gagcttttgg ggcggttttc
    37201 gtgtcagcct tgaacagatt gagttctggc agggcggtga gcatcgcctg catgaccgct
    37261 ttttgtacca gcgagaaaat gatgcgtgga agattgatcg tcttgcaccc tgaaaagatg
    37321 caaaaatctt gctttaatcg ctggtactcc tgattctggc actttattct atgtctcttt
    37381 cgcatctggc gaaaagtcgt gtaccggcaa aggtgcagtc gttatataca tggagatttt
    37441 gatggcaagc agtaacttga ttaaacaatt gcaagagcgg gggctggtag cccaggtgac
    37501 ggacgaggaa gcgttagcag agcgactggc gcaaggcccg atcgcgctct attgcggctt
    37561 cgatcctacc gctgacagct tgcatttggg gcatcttgtt ccattgttat gcctgaaacg
    37621 cttccagcag gcgggccaca agccggttgc gctggtaggc ggcgcgacgg gtctgattgg
    37681 cgacccgagc ttcaaagctg ccgagcgtaa gctgaacacc gaagaaactg ttcaggagtg
    37741 ggtggacaaa atccgtaagc aggttgcccc gttcctcgat ttcgactgtg gagaaaactc
    37801 tgctatcgcg gcgaacaact atgactggtt cggcaatatg aatgtgctga ccttcctgcg
    37861 cgatattggc aaacacttct ccgttaacca gatgatcaac aaagaagcgg ttaagcagcg
    37921 tctcaaccgt gaagatcagg ggatttcgtt cactgagttt tcctacaacc tgttgcaggg
    37981 ttatgacttc gcctgtctga acaaacagta cggtgtggtg ctgcaaattg gtggttctga
    38041 ccagtggggt aacatcactt ctggtatcga cctgacccgt cgtctgcatc agaatcaggt
    38101 gtttggcctg accgttccgc tgatcactaa agcagatggc accaaatttg gtaaaactga
    38161 aggcggcgca gtctggttgg atccgaagaa aaccagcccg tacaaattct accagttctg
    38221 gatcaacact gcggatgccg acgtttaccg cttcctgaag ttcttcacct ttatgagcat
    38281 tgaagagatc aacgccctgg aagaagaaga taaaaacagc ggtaaagcac cgcgcgccca
    38341 gtatgtactg gcggagcagg tgactcgtct ggttcacggt gaagaaggtt tacaggcggc
    38401 aaaacgtatt accgaatgcc tgttcagcgg ttctttgagt gcgctgagtg aagcggactt
    38461 cgaacagctg gcgcaggacg gcgtaccgat ggttgagatg gaaaagggcg cagacctgat
    38521 gcaggcgctg gtcgattctg aactgcaacc ttcccgtggt caggcgcgta aaactatcgc
    38581 ctccaatgcc atcaccatta acggtgaaaa acagtccgat cctgaatact tctttaaaga
    38641 agaagatcgt ctgtttggtc gttttacctt actgcgtcgc ggcaaaaaga attactgtct
    38701 gatttgctgg aaataatgca ttaagtggaa agggggagtg agaaatcact ccccctggtt
    38761 tttatacagg gaacatgatg aaaaatattc tcgctatcca gtctcacgtt gtttatggtc
    38821 atgcgggtaa cagtgcggca gagtttccta tgcgccgcct gggcgcgaac gtctggccgc
    38881 tgaacaccgt tcaattttct aatcacaccc aatacggcaa atggactggc tgcgtgatgc
    38941 cgcccagcca tttaaccgaa attgtgcaag gcattgccgc cattgataaa ttacacacct
    39001 gtgatgccgt gttaagtggc tatctgggat cggcggagca gggtgaacat atcctcggta
    39061 tcgtccgtca ggtgaaagcc gcgaatccgc aggcgaaata tttttgcgat ccggtaatgg
    39121 gtcatccgga aaaaggctgt atcgttgcac cgggtgtcgc agagtttcat gtgcggcacg
    39181 gtttgcctgc cagcgatatc attgcgccga atctggttga gctggaaata ctctgtgagc
    39241 atgcggtaaa taacgtcgaa gaagcggttc tggcagcgcg cgaactcatt gcgcaagggc
    39301 cacaaattgt gttggttaaa cacctggcgc gagctggcta cagccgtgac cgttttgaaa
    39361 tgctgctggt caccgccgat gaagcctggc atatcagccg tccgctggtg gattttggta
    39421 tgcgccagcc ggtaggtgtt ggtgatgtga cgagcggttt actgctggtg aaactgcttc
    39481 agggggcaac gctgcaggag gcgctggaac atgtgaccgc tgcagtctac gaaatcatgg
    39541 tgaccaccaa agcaatgcag gaatatgagc tgcaagtggt ggctgctcag gatcgtattg
    39601 ccaaaccaga acattacttc agcgcaacaa agctctgaaa tatctcattt cggcccacaa
    39661 cgacctgtgg gccgatggat attactttaa gccttccgct gacagcgcgt cttgtacttc
    39721 cggacgttca gccatacgtt gcataaatgc tgcaatgtgc tctaaccctt ccagattcag
    39781 tttcaccgcg tatgcccagc gcagaaccgt aaacagatag gcatcagcaa ttgtaaatct
    39841 ttgcccgcag atccagtgct catccttcag tgcctcgttc acatattgca gcttcttctc
    39901 cagctgcgcg cgaactgtcg gtttgtactc ttccggtgta tcagggcgaa atagaggtgt
    39961 gaaaccttta tgcagctcgg tggcgatgta attcagccat tcgatggttt tatagcggga
    40021 aatactgttt accggtgcca gcaactggcg gtcggggacg ctgtcggcaa gatactgcat
    40081 aatcgctacg ccttccgtca gcaaagtacc gtcatccagc agcaatgcag gcacctgccc
    40141 cttagggtta acggcaaagt aatcgtcacc gttttcgaga cgttttttca ttaaatccac
    40201 actgacgagg gtaaaatcct ttccgctctc acgcagggtg atatgggaag cgagagagca
    40261 ggcacccggt ttgtagaaca atttcatcgg taactccttt tgtctgtggt ttaaagtatg
    40321 gtagtgcgtt gctgaacaaa aaaaaagccg ctaacgaaaa gttagcggct tgtaaaagag
    40381 tttccctgaa ttttacgcta cagcagcttt cgccgctttg tctgcagcgt catcctgcgt
    40441 catacggtgc agtttaggcg cggtcagcag catcagtact gcaataatgg cagtagcgac
    40501 accaatctgc aagaatacgc gaccataaac ttccagtgac atcagcgggt cggtaacgtt
    40561 atccggcaca gccatcatac ccgcaacata accaccaatc aggtttgcac cggcagtggt
    40621 caggaaccag ctacccataa tgaagcccat cagacgctgc ggaacgagtt gtgcaaccat
    40681 tgccagaccc agaccagaga tcatcagttc cccgatgctc tgcaggccat agcttgcgac
    40741 cagccagctt acagacacga taccagcgtc agacgcgaat ttcgcaccca gcggcagaat
    40801 caggaacgca ccagaacaca tcaccatgcc gattgcaaac ttggttggca tcggcagggt
    40861 atcgcccatc ttgttataga tagcggccag aatcggacta ccgatgatga tccagaacgg
    40921 gttcagtgcc tgatactgct caggttctac ggccagaccc agaatggagt gctcaacgtt
    40981 acgaatcgca aagaagttca gtgacgttgg catctggctg tacagcacga agaagataat
    41041 ggcttcgagc atcaggatga aggcaacgat cattttacga cgcgcagcac ctttcatcgc
    41101 gaaggcttct ttaccgaaga taaccacgat accgaaggca acaacgccca gcgccatacg
    41161 cgcaacttcc tgattgtgca gcagccaggt ggcgatagcg atcagtgcca caacaccaat
    41221 aatggtcagc agcaggttac ggtagttgat aggctcgaag tctggttttg aaccgtactg
    41281 tttaacccag cgttggcaga aggcgaagtt aacgatagtg atcagcaggc ctacaacgct
    41341 caacgcaaac gcaacactcc agccgtattt cgcggccagc cacggcgtag caatcataga
    41401 gaagaaagag ccgatgttga cggacatgta gtacatggtg aatgcaccgt ccagacgcgg
    41461 gtcgtttttc tcatagcatg tagaaagcag agaagacggg ttagctttaa acaggccgtt
    41521 accgaccgca atagccgcca tacccatata aacgataccg gcgtcgtgac cagaccaggc
    41581 aaccagagca taaccaatcg ccagcacaat agcgccgagc ataattacgc gtttagtacc
    41641 cagtaccttg tcacctaacc agccgccgat agcgaccaga ccataaacca gggcactaaa
    41701 ggaagagaaa agggtgattg aatccgcttc agacataccc agttgtttaa ccaggtaaac
    41761 agccataatt ccttgtaggc cgtaataacc aaaacgttcc cataactcaa tcgagaagat
    41821 gagatagaac gccttcggtt gtttgaaagc gttcaaactg acgctttcag ttggtttttg
    41881 gtttgcagtg gacactttta cctctttttt tacatcccat attaacgggg gtgctttctc
    41941 gcgttatgtc taaaaaacat acgcgcaatt gttatagtgg gaggggaaac ggcaggtaat
    42001 gttcactatc ttgcactttc agacaagtcc tttgtaataa tctgttacat ataactgagg
    42061 cggggaattc aaccattccg ggaaatgtta tccagcgtta aatttttcaa aaaacgtaac
    42121 ggcagatttt ttcactgcac tgagaggggg gtgatggctt aacggcgata tgttctacta
    42181 tttagcgttt ttactagtga gatagtccag tttctgaaaa atagccagtg taatgttttg
    42241 taggtcaata tttgttgcgt gtgtaaattt aaatgatttt aacctgattc ttgcaatgtt
    42301 tttacattgc gacagcatcg tgatcttgat cacgaatgaa tagaaaattt cgctattaaa
    42361 aaaacaatta acct
//
LOCUS       DAZKIV010000021        41756 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZKIV010000021 DAZKIV010000000
VERSION     DAZKIV010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41756
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.021"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..803
                     /locus_tag="ACHOT6_001203"
     CDS             <1..803
                     /locus_tag="ACHOT6_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9946452.1"
                     /translation="LQPYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMS
                     INENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHG
                     ITFDDQRKRYIIGPITVSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRS
                     FNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKH
                     AGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            993..1106
                     /gene="yhiY"
                     /locus_tag="ACHOT6_001204"
     CDS             993..1106
                     /gene="yhiY"
                     /locus_tag="ACHOT6_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFP9946453.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            1169..2236
                     /gene="yhiI"
                     /locus_tag="ACHOT6_001205"
     CDS             1169..2236
                     /gene="yhiI"
                     /locus_tag="ACHOT6_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9946454.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKMGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2233..4968
                     /gene="rbbA"
                     /locus_tag="ACHOT6_001206"
     CDS             2233..4968
                     /gene="rbbA"
                     /locus_tag="ACHOT6_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFP9946455.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILQLKKQEG"
     gene            4968..6092
                     /gene="yhhJ"
                     /locus_tag="ACHOT6_001207"
     CDS             4968..6092
                     /gene="yhhJ"
                     /locus_tag="ACHOT6_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946456.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6191..6466
                     /locus_tag="ACHOT6_001208"
     CDS             6191..6466
                     /locus_tag="ACHOT6_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9946457.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6463..6822
                     /locus_tag="ACHOT6_001209"
     CDS             6463..6822
                     /locus_tag="ACHOT6_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9946458.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6942..7343)
                     /gene="nikR"
                     /locus_tag="ACHOT6_001210"
     CDS             complement(6942..7343)
                     /gene="nikR"
                     /locus_tag="ACHOT6_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFP9946459.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7349..8155)
                     /gene="nikE"
                     /locus_tag="ACHOT6_001211"
     CDS             complement(7349..8155)
                     /gene="nikE"
                     /locus_tag="ACHOT6_001211"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFP9946460.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVTLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLNAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGEKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8152..8916)
                     /gene="nikD"
                     /locus_tag="ACHOT6_001212"
     CDS             complement(8152..8916)
                     /gene="nikD"
                     /locus_tag="ACHOT6_001212"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFP9946461.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLKRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLRTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSHGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(8916..9749)
                     /gene="nikC"
                     /locus_tag="ACHOT6_001213"
     CDS             complement(8916..9749)
                     /gene="nikC"
                     /locus_tag="ACHOT6_001213"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFP9946462.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGS
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9746..10690)
                     /gene="nikB"
                     /locus_tag="ACHOT6_001214"
     CDS             complement(9746..10690)
                     /gene="nikB"
                     /locus_tag="ACHOT6_001214"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFP9946463.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10690..12264)
                     /gene="nikA"
                     /locus_tag="ACHOT6_001215"
     CDS             complement(10690..12264)
                     /gene="nikA"
                     /locus_tag="ACHOT6_001215"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9946464.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12375..12962)
                     /gene="acpT"
                     /locus_tag="ACHOT6_001216"
     CDS             complement(12375..12962)
                     /gene="acpT"
                     /locus_tag="ACHOT6_001216"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFP9946465.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPEMPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13017..14066)
                     /gene="yhhT"
                     /locus_tag="ACHOT6_001217"
     CDS             complement(13017..14066)
                     /gene="yhhT"
                     /locus_tag="ACHOT6_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFP9946466.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNSVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14198..15415
                     /gene="yhhS"
                     /locus_tag="ACHOT6_001218"
     CDS             14198..15415
                     /gene="yhhS"
                     /locus_tag="ACHOT6_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946467.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15419..15976)
                     /gene="dcrB"
                     /locus_tag="ACHOT6_001219"
     CDS             complement(15419..15976)
                     /gene="dcrB"
                     /locus_tag="ACHOT6_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFP9946468.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16049..16714)
                     /gene="yhhQ"
                     /locus_tag="ACHOT6_001220"
     CDS             complement(16049..16714)
                     /gene="yhhQ"
                     /locus_tag="ACHOT6_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFP9946469.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16935..17180
                     /gene="tusA"
                     /locus_tag="ACHOT6_001221"
     CDS             16935..17180
                     /gene="tusA"
                     /locus_tag="ACHOT6_001221"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFP9946470.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17282..19480)
                     /gene="zntA"
                     /locus_tag="ACHOT6_001222"
     CDS             complement(17282..19480)
                     /gene="zntA"
                     /locus_tag="ACHOT6_001222"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFP9946471.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTAVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAAEEPQASRLKENLSLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19554..20180)
                     /gene="yhhN"
                     /locus_tag="ACHOT6_001223"
     CDS             complement(19554..20180)
                     /gene="yhhN"
                     /locus_tag="ACHOT6_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFP9946472.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20321..20680
                     /gene="yhhM"
                     /locus_tag="ACHOT6_001224"
     CDS             20321..20680
                     /gene="yhhM"
                     /locus_tag="ACHOT6_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFP9946473.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLEAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20683..20952)
                     /gene="yhhL"
                     /locus_tag="ACHOT6_001225"
     CDS             complement(20683..20952)
                     /gene="yhhL"
                     /locus_tag="ACHOT6_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFP9946474.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20942..21538)
                     /gene="rsmD"
                     /locus_tag="ACHOT6_001226"
     CDS             complement(20942..21538)
                     /gene="rsmD"
                     /locus_tag="ACHOT6_001226"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFP9946475.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21688..23184
                     /gene="ftsY"
                     /locus_tag="ACHOT6_001227"
     CDS             21688..23184
                     /gene="ftsY"
                     /locus_tag="ACHOT6_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFP9946476.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETA
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23187..23855
                     /gene="ftsE"
                     /locus_tag="ACHOT6_001228"
     CDS             23187..23855
                     /gene="ftsE"
                     /locus_tag="ACHOT6_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFP9946477.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23848..24906
                     /gene="ftsX"
                     /locus_tag="ACHOT6_001229"
     CDS             23848..24906
                     /gene="ftsX"
                     /locus_tag="ACHOT6_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFP9946478.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25151..26005
                     /gene="rpoH"
                     /locus_tag="ACHOT6_001230"
     CDS             25151..26005
                     /gene="rpoH"
                     /locus_tag="ACHOT6_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFP9946479.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26276..27379
                     /gene="livJ"
                     /locus_tag="ACHOT6_001231"
     CDS             26276..27379
                     /gene="livJ"
                     /locus_tag="ACHOT6_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFP9946480.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27578..27961)
                     /gene="panM"
                     /locus_tag="ACHOT6_001232"
     CDS             complement(27578..27961)
                     /gene="panM"
                     /locus_tag="ACHOT6_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFP9946481.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            28385..29494
                     /gene="livK"
                     /locus_tag="ACHOT6_001233"
     CDS             28385..29494
                     /gene="livK"
                     /locus_tag="ACHOT6_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFP9946482.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            29542..30468
                     /gene="livH"
                     /locus_tag="ACHOT6_001234"
     CDS             29542..30468
                     /gene="livH"
                     /locus_tag="ACHOT6_001234"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFP9946483.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            30465..31742
                     /gene="livM"
                     /locus_tag="ACHOT6_001235"
     CDS             30465..31742
                     /gene="livM"
                     /locus_tag="ACHOT6_001235"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFP9946484.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            31739..32506
                     /gene="livG"
                     /locus_tag="ACHOT6_001236"
     CDS             31739..32506
                     /gene="livG"
                     /locus_tag="ACHOT6_001236"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFP9946485.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            32508..33221
                     /gene="livF"
                     /locus_tag="ACHOT6_001237"
     CDS             32508..33221
                     /gene="livF"
                     /locus_tag="ACHOT6_001237"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFP9946486.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGRTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            33618..34934
                     /gene="ugpB"
                     /locus_tag="ACHOT6_001238"
     CDS             33618..34934
                     /gene="ugpB"
                     /locus_tag="ACHOT6_001238"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFP9946487.1"
                     /translation="MKSLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            35032..35919
                     /gene="ugpA"
                     /locus_tag="ACHOT6_001239"
     CDS             35032..35919
                     /gene="ugpA"
                     /locus_tag="ACHOT6_001239"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFP9946488.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVALFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            35916..36761
                     /gene="ugpE"
                     /locus_tag="ACHOT6_001240"
     CDS             35916..36761
                     /gene="ugpE"
                     /locus_tag="ACHOT6_001240"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFP9946489.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            36763..37833
                     /gene="ugpC"
                     /locus_tag="ACHOT6_001241"
     CDS             36763..37833
                     /gene="ugpC"
                     /locus_tag="ACHOT6_001241"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFP9946490.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            37830..38573
                     /gene="ugpQ"
                     /locus_tag="ACHOT6_001242"
     CDS             37830..38573
                     /gene="ugpQ"
                     /locus_tag="ACHOT6_001242"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9946491.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDLIGPNFTAQ"
     gene            complement(38560..39000)
                     /gene="yhhA"
                     /locus_tag="ACHOT6_001243"
     CDS             complement(38560..39000)
                     /gene="yhhA"
                     /locus_tag="ACHOT6_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFP9946492.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            39120..40862
                     /gene="ggt"
                     /locus_tag="ACHOT6_001244"
     CDS             39120..40862
                     /gene="ggt"
                     /locus_tag="ACHOT6_001244"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFP9946493.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(40900..41184)
                     /gene="yrhB"
                     /locus_tag="ACHOT6_001245"
     CDS             complement(40900..41184)
                     /gene="yrhB"
                     /locus_tag="ACHOT6_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFP9946494.1"
                     /translation="MITYHDAFAKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFL
                     ETGDEAARLAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
     gene            complement(41384..41539)
                     /gene="yrhD"
                     /locus_tag="ACHOT6_001246"
     CDS             complement(41384..41539)
                     /gene="yrhD"
                     /locus_tag="ACHOT6_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFP9946495.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         9777 a        10724 c        11611 g         9644 t
ORIGIN      
        1 gtcttcagcc ttatttggtt attaataaag acgcagtcac gccatgtacg tttctgggcg
       61 accagactgg cgatcgattt agtaatatct gtggcgataa atttatcatc gatttattaa
      121 aacgcataat gagtatcaat gaaaacgttc atgtattagc tggaaatcat gaaacgaatt
      181 gcaatggtaa ctatatgcag aacttcacgc gcatgaaacc acttgatgag gatacctact
      241 ctgggataaa agattatcct gtatgtttct atgaccccaa atacaaaata atggcaaatc
      301 atcatgggat aacttttgat gatcagcgaa aacgttacat tataggaccg ataactgtct
      361 ctattgatga aatgaccaat gctctcgatc cggtcgaatt agcggaaatt attaataaaa
      421 agcatcatgc tataattaat ggtaaaaagt ttaaaactag ccgggctatc tcttgtcgat
      481 cctttaatcg ttatttttct gtttcgacag attacagacc taaacttgaa gcactattag
      541 catgctcaca aatgttaggt ataaatcaag ttgttgcaca taacggaaat ggtgggagag
      601 aacgcatagg tgaaacgggg acagttttag gtcttaacgc ccgtgacagt aaacatgctg
      661 gaagaatgtt cagcatgcat aattgccaaa ttaaccctgg tgcagggcct gagataacca
      721 ctccctggaa gtcttaccag catgaaaaaa acagaaatgg acttatgcct ttgatcagaa
      781 ggcgcacaat gttacagctc tgaaccatag acaacatgaa tattaatgtt actatctggt
      841 tgagactgtt gataaaacgt aaaaaggaat gctctgttcc tgcaactgaa caggcgtgaa
      901 acgcgatcca acaggatgga taccgttttg tcagccagtt ttatagatca cacttatgag
      961 tatgcgtgtt tcatttacgg ccaggaatgc tgatgatgac aacattacta cccgttttca
     1021 ctaagccttc cccgttggcg ctcaatgctc tgcgcgctgg tcgtatttgt cgtttccttc
     1081 ttatcccgga tggaagaatc cgctgaacgt cgttcggcgg cagttgtaac acgcgtcgtt
     1141 tgcttacagg gattatggcg ggtcgattat ggataagagt aagcgccatc tggcgtggtg
     1201 ggttgtcggg gcactggcgg tggctgccgt cgtggcgtgg tggctgttgc gcccggcggg
     1261 cgtgccggaa ggctttgctg tcagcaatgg gcgcattgaa gcgacggaag tggatatcgc
     1321 cagcaaaatt gccgggcgta tcgacaccat tctggtgaaa gaaggccagt ttgttcgcga
     1381 aggcgaagtg ctggcgaaga tggatactcg cgtgttgcag gaacagcgac tggaagccat
     1441 cgcgcaaatc aaagaggcac aaagcgccgt tgccgccgcg caggctttgc tggagcaacg
     1501 acaaagtgaa actcgtgccg cacagtcgct ggtcaatcaa cgccaggccg aactggactc
     1561 cgttgccaaa cgtcatacgc gttcccgctc gctcgcccaa cgaggggcta tttctgcgca
     1621 acagctggat gacgatcgcg ccgccgctga gagcgcccga gcggcgctgg aatcggcaaa
     1681 agctcaggtt tctgcttcta aagccgctat agaggcggca cgcaccaata tcattcaggc
     1741 gcaaacccgc gtcgaagcgg cacaagccac tgaacggcgc attgccgcag atatcgatga
     1801 cagcgaactg aaagccccgc gtgacggacg cgtgcagtat cgggttgccg agccagggga
     1861 agtgctggcg gcaggcggtc gggtgctgaa tatggtcgat ctcagcgacg tctatatgac
     1921 tttcttcctg ccaaccgaac aggcgggcac gctaaaactg ggcggtgaag cacggctgat
     1981 cctcgatgcc gcgccagatc tgcgtattcc ggcgaccatc agttttgtcg ccagcgtcgc
     2041 ccagttcacg ccaaaaaccg tcgaaaccag cgatgaacgg ctgaaactga tgttccgcgt
     2101 caaagcgcgt atcccaccgg aattactcca gcagcatctg gaatatgtca aaatgggctt
     2161 gccgggcgta gcgtgggtgc gggtgaatga agaacttccg tggcctgacg acctggtggt
     2221 gaggttgccg caatgacgca tctggaactg gttcccgtcc cgcctgtcgc gcaactggcg
     2281 ggcgtgagcc agcattatgg aaaaaccgtt gcgctgaaca atatcactct cgatattccg
     2341 gcccgctgta tggtcgggct gattggcccg gacggcgtcg ggaagtcgag cttgttgtcg
     2401 ttgatttccg gtgcccgcgt cattgagcag ggcaatgtga tggtgctggg cggcgatatg
     2461 cgcgacccga agcatcgccg cgacgtctgc ccgcgcattg cctggatgcc gcaggggctg
     2521 ggcaaaaatc tctaccacac cttgtcggtg tatgaaaacg tcgatttttt cgcccgcctg
     2581 ttcggtcacg acaaagcgga gcgggaagtg cgaattaatg agttgctgac cagcaccggg
     2641 ttagcaccgt ttcgcgatcg tccggcaggt aaactctccg gcgggatgaa gcaaaaactt
     2701 gggctgtgct gcgcgttaat ccacgacccg gaactgttga tccttgatga gccaacaacg
     2761 ggggttgacc cgctctcccg cgcccagttc tgggatctga tcgacagtat tcgccagcgg
     2821 cagagcaata tgagtgtgct ggtcgccacc gcctatatgg aagaggccga acgcttcgac
     2881 tggctggtag cgatgaatgc cggagaagtg ctggcaactg gcagcgccga agagctgcgg
     2941 cagcaaacgc aaagcgccac gctggaagag gcgtttataa atctgttacc gcaagcgcaa
     3001 cgccaggcgc atcaggcggt agtgatccca ccgtatcaac ctgaaaatgc agagattgcc
     3061 atcgaagcgc gcgatctgac catgcgtttt ggttccttcg ttgccgttga tcacgtcaat
     3121 ttccgcattc cacgcgggga gatttttggt tttcttggat cgaacggctg cggtaaatcc
     3181 accaccatga aaatgctcac tggactgctg cccgccagcg aaggtgaggc gtggctgttt
     3241 gggcaaccgg ttgatccaaa agatatcgat acccgccgtc gggtgggcta tatgtcgcag
     3301 gcgttttcgc tctataacga actcaccgtg cggcaaaacc ttgagttaca tgcccgtttg
     3361 tttcacatcc cggaagcgga aattcccgca agagtggctg aaatgagcga gcgttttaag
     3421 ctcaacgacg ttgaagatat tctgccggag tcattgccgc tcggcattcg ccagcggctt
     3481 tcgctggcgg tggcggtgat tcatcgcccg gagatgttaa tcctcgatga gcctacttct
     3541 ggtgtcgatc cggtggcgag ggatatgttc tggcagttga tggtcgatct ctcgcgccag
     3601 gacaaagtga ctatcttcat ctccacccac ttcatgaacg aagcggaacg ttgcgaccgc
     3661 atctcactga tgcacgccgg aaaagtgctt gccagcggta caccgcagga actggttgag
     3721 aaacgcggag ccgccagtct ggaagaggca tttatcgcct atttgcagga agcggcaggg
     3781 cagagcaacg aagccgaagc gccgcccgtg gtacacgaca ccacccacgc gccgcgtcag
     3841 ggatttagcc tgcgccgtct gtttagctac agccgccgcg aagcgctgga actgcgacgc
     3901 gatccagtac gttcgacgct ggcgctgatg ggaacggtga tcctgatgct gataatgggt
     3961 tacggcatca gtatggatgt ggaaaacctg cgctttgcgg tgctcgaccg cgaccagacc
     4021 gtcagtagcc aggcgtggac actcaacctc tccggttccc gttactttat cgaacagccg
     4081 ccgctcacca gttatgacga gcttgatcgt cggatgcgtg cgggcgatat cacggtggcg
     4141 attgagattc cgcccaattt cgggcgcgat atcgcgcgtg gtacgcctgt ggaactcggc
     4201 gtctggatcg acggagcgat gcccagtcgt gccgaaacgg taaaaggtta cgtgcaggca
     4261 atgcaccaga gttggcttca ggatgtggcg agccgacaat cgacacccgc cagccaaagc
     4321 gggctgatga acattgagac gcgttatcgc tataacccgg acgtaaaaag cctgccagcg
     4381 attgttccgg cggtgatccc gcttctgctg atgatgatcc cgtcaatgct aagcgccctt
     4441 agcgtggtgc gggaaaaaga gcttgggtcg attatcaacc tttacgtgac ccccaccacg
     4501 cgtagtgaat ttttgcttgg taaacagttg ccatacatcg cgctggggat gctgaacttt
     4561 ttcctgctct gcggcctgtc ggtgtttgtg tttggcgtac cgcataaagg cagtttcctg
     4621 acgctcaccc tggcggcgct gctgtatatc atcattgcca ccggaatggg gctgctgatc
     4681 tccaccttta tgaaaagcca gattgccgcc attttcggaa cggcgattat cacattgatc
     4741 ccagcgacac agttttccgg gatgatcgat ccggtagctt cgctggaagg gcctggacgt
     4801 tggatcggcg aggtttaccc gaccagtcat tttctgacta tcgcccgcgg aacgttctcg
     4861 aaagcgctgg atctgactga tttgtggcaa ctttttatcc cgttgctgat agccatcccg
     4921 ctggtgatgg gcttaagtat cctgcagctg aaaaaacagg agggatgatg cgccatttac
     4981 gcaatatttt taatctgggt atcaaagagt tgcgcagtct gctcggtgat aaagcgatgc
     5041 tgacgctgat tgtcttctcg tttacggtgt cggtgtattc gtcagcgacc gttacgccag
     5101 gatcgttgaa cctcgcgccg atcgccattg ccgatatgga tcaatcgcag ttatcgaacc
     5161 ggatcgttaa cagcttctat cgcccgtggt ttttgccacc ggagatgatc accgccgatg
     5221 agatggatgc cggactggac gccggacgct ataccttcgc gataaatatt ccgcctaatt
     5281 ttcagcgtga tgtcctcgcc ggacgccagc cagatattca ggtgaacgtc gatgccacgc
     5341 gcatgagcca ggcatttacc ggcaatgggt atatccagaa tattatcaac ggtgaagtga
     5401 acagctttgt cgcgcgctac cgtgataaca gcgaaccgtt ggtatcgctg gaaacccgga
     5461 tgcgctttaa cccgaacctc gatcccgcgt ggtttggcgg ggtgatggcg atcatcaaca
     5521 acattaccat gctggcgatt gtattgaccg gatcggcgct gatccgcgag cgtgaacacg
     5581 gcacggtgga acacttactg gtgatgccga taacgccgtt tgagatcatg atggcgaaga
     5641 tctggtcgat ggggctggtg gtgctggtgg tatcgggatt atcgctggtg ctgatggtga
     5701 aaggtgtact gggcgtaccg attgaaggct cgatcccgct gtttatgctg ggcgtggcgc
     5761 tcagtctgtt tgccaccacg tcaatcggca tttttatggg gacgatagcg cgttcaatgc
     5821 cgcaactggg gctgctggtg attctggtgc tgctgccgct gcaaatgctt tccggtggat
     5881 ccacgccgcg cgaaagtatg ccgcagatgg tgcaggacat tatgctgacc atgccgacga
     5941 cacactttgt tagcctcgcg caggccattc tctaccgggg tgccggattc gaaatcgtct
     6001 ggccgcagtt cctgacgctg atggcaattg gcggcgcgtt tttcaccatt gcgctgctgc
     6061 gattcaggaa gacgattggg acaatggcgt aaggaaggga aacttgactt catactgtga
     6121 tgtcatacta ttgatgtcat aaagtgaagt cataagaaca tgccatgaga acatgccatg
     6181 agaacatgcc atgagaacca aggtacaggc tttacggaag aaacaaaaaa atacattgga
     6241 tcaaatattt aaaactcctg ttcctcaagg gattaagtgg tctgatatag agtcactggt
     6301 taaagcatta ggcggagaaa ttaaggaagg aagaggttcg cgttgtaagt tcatactaaa
     6361 tatgagcgtt gcgtgtttcc atcggcctca tccgtcgcca gataccgata aaggcgctgt
     6421 agaaagcgtg cgtgactggt tactaagtat aggggtaaaa ccatgatcaa actgaagacg
     6481 ccaaactcta tggagattgc cgggcaacct gcagttatca cttacgtgcc agaactgaat
     6541 gcatttcgtg gcaaattcct gggcttgtca ggttattgtg attttgtctc tgacagcatt
     6601 caggggctgc aaaaagaagg ggaactttcc ctgcgagagt atcttgagga ttgtaaagct
     6661 gcgggtattg agccgtatgc gcgtacagaa aagattaaga cctttacgtt acgctatcca
     6721 gaatcgctga gtgaacgcct taataatgcg gctgcgcagc aacaagtctc tgttaatacc
     6781 tatattattg aaacactgaa tgagcgtttg aatcatttat aacttttagc ggcgtacatt
     6841 acgcagaata tctgccttta caggatctat ctatgcaact gaacttaatt ttataaatta
     6901 agttcagtta atatcttttc tttcctcgtt tcagaaataa ctcaatcttc cttcggcagg
     6961 cactgcaaat gcccgtgccg cacgccgcgc tgggcgataa cgtcatcggc aaaatgctgc
     7021 acgtcgccca tatcaccttt cagcacggcg atttccagac agtcgtcgtg attgatatgc
     7081 acatgcagcg tggcgacgga gagatcgtgg tgatggtgct gggtggagac aatgcggctg
     7141 gctaagtcgc gtttttcgtg ttcatacaca taggagagca ccgcgaaacc ttgcgtgccg
     7201 tgctgctgag tggcctcctg ggccagggcg ctacgcagaa tatcgcggat agcttcggaa
     7261 cggttgttat aaccacgacg ctggctcagg ctgtccagcg tctccagtaa atcgtcatca
     7321 agcgtgatgg tgactcgttg catttgcgtt aaaccttttc tgtggtgcga cggcgcacgg
     7381 ggaatgcggg taataccgcg ttttgtagca cacgtccggc gtcagaggaa aaagttaatt
     7441 tctctcccac cacctgggtt tcgacgattt gtccgttgtc catcaccatc acccgctgac
     7501 agaagcgttc caccaggcgt aagtcgtggg tgatgaacag gcaggcggtg ccaaactgtt
     7561 gttgtagctt tttcagcagg cgaatgacgc ccgcctgtaa cacgagatca aggttagaaa
     7621 cggcttcatc cagaatcagt agtttcggtt cgaccgccag cgcgcgagct aggcagacgc
     7681 gctggagctg gccgccgctt aactgcggtg ggcgcttgtc gagcacgctg tcgtcgagat
     7741 cgaccgcatt cagcatttcg ctggcgcgcg ccagttgttc ggattttttc agcgatagca
     7801 ggtgtcgcat cggttcacgc aggatctcgc gcacggtttt gcgcggattc acggcactga
     7861 tggagtcctg aaacaccatc tgaatatcac ggcggaaggc tttacgttgg gcgcgattaa
     7921 gtttcgccag cgattcgccg cgccagctaa tattcccttg tgagggcgat tctaagccta
     7981 ccagcagccg cgcgagagtg ctttttccgc agccgctgcg ccccagcagg gcgacggttt
     8041 caccgctttt cagggtcagc gtaacgttat taagcaccgc ctgatgctgg tgcttaccgc
     8101 taaatccacc gtgcgcatag tgatgggaaa ggccggagac gttaagtaaa gtcatgatgc
     8161 cagctccata ccgtagaggg cgagatgagc ggaaacaagg ctgcgcgtca ccgcatgttt
     8221 gggggcgtta aacagcgttt cgacatcgcc ctgttcgaca atcttaccgt gcgacatcac
     8281 cgccacgtca tccgccagac gcgccactac acccatatca tgggtgacca gcagcatccc
     8341 cggtgcctgt ttttgcataa tgctttccag cagatcgagg atgcgcgcct gtgctaccac
     8401 gtcgaggtcg gtggttggtt catcggcgat gataaacggt gattcgcaaa gcaccgccat
     8461 cgcaatcatc atgcgctgca acatgccgcc gctcatctcg aacgggtaca gcttcagcac
     8521 gcgtgcggcg ttttccagcc ccaccgcttc tatggcagcg gtaagcgtag cgtcatcggc
     8581 gggtttcccc agcgccaggc aggtttcacg cgcgtgggtg tgcatggtgc gtagcggatt
     8641 aaaggcgctg cgcgggttct gcatgatggt ggcaattttg atgccgcgca gggcgcaagg
     8701 cgaaaccggt ttgccatcgg ctaaaatttc ccccgccgtc tggcgaacgc cagcgggcag
     8761 aatgcccagc gtcgcggcgc aggtcagtga tttcccgctg ccgctaccgc cgactaacgc
     8821 cagcacacgc ccgcgtttaa gggttaacga tacgccgtgc accagcggct gcgcggcctg
     8881 tagcgcgata ttacgtagtt caatctgttg cggcattagt gtgcgtgctc cgtcaccaga
     8941 tgagggtcca gatgatcgcg cagtgcgtca cccaccaggt taaaggccat cacgctgata
     9001 aacagcgcca gccccggcca gaacatttgc agcggctggg tccagatata ctggcgcgcg
     9061 tcgttaatca tcacgcccca ttcggcggtc ggtgcggtca cgccgaggcc gaggaaggac
     9121 atccctgcga cgtgcagcat catatggccg atatccagcg ttgccagcac cagcagcgag
     9181 gggatcaccg cacctgccag atgatctaca aacactcgca catggcccgc gccagaaagc
     9241 cgtgatgcca gcacaaactc gcgctggcgc agtgaaatca ccaggctgcg caccatgcgt
     9301 gcataccacg cccagtgcga cagggcgatg gcgataatta cattggtcag cccggtgccg
     9361 agcacgccaa ccataaagaa cgacagaatc gaggtcggga aggtcataaa catatcggcg
     9421 acgcgcatgg tggcctgatc aacgctcccg ccaatcaacc cggcgctgcc gccaataacc
     9481 agccctaatg tcagcaccag cagcaggcag gccattaccg aaccgagcga cacgcgggtc
     9541 gctgccatca gccgcgagaa aatatcgcga cctaagtgat cggtgcccag ccagtgctgc
     9601 gcatccggcg aaagcaggcg cgacggcaaa tcaatcgcct gggggtcata cggcagccac
     9661 cactggctgg tgagcgcaat cagcgccagc agggcgataa tgatcagcgc caggcgtacc
     9721 gaccagcggg aagagaggaa aaagttcacg cgtgcgctcc ttcatgacga cgaatgcgcg
     9781 ggtccagcgc ggcgttgagc aaatcgacaa tcaaattaca gaccacaaaa accaccacca
     9841 tcatcagcgt aaagcactga atcaccggat agtcacggtt aaaaatcgcc gacaccgcat
     9901 agcgcccgac gcccggccag gcaaagatgt tttcgataat catcgtcccg ccgatcagtt
     9961 cgccgatgtg catccccacg gcggtgatca tcggcagcga ggcattacgc agaatatggc
    10021 ggcgttcggt ctgtttgtcg ttcaggccgc gcagacgcgc ccaggtgacg tgacgctgac
    10081 cggcgacgtc cagcatactg gcgcgcagta aacgcgcgtt aatcgccagc gacataaagg
    10141 caatggaaac cgcaggcaaa atgatgtgct gccagccgcc gtaacccatc gcgggtagcc
    10201 attgcagata caccgaaaac gccatcacca gtaaaaacgc cagccagaag ttaggcatcg
    10261 acacgccaag aaacgcgata aagcgcacgg cgaaatccgg caaacgatcg cggtggcgcg
    10321 ccgcccagat accgagcggt acggaagtga gcagaattaa taccagcgcc gcacctgcaa
    10381 gttccagcgt ggcgggcagg aagttcagca tatcgtccag taccgggcgt tggctggcga
    10441 atgagatacc aaagtcaaga tgcagcgcct tccacaacca ggtaccgtac tggacgtaca
    10501 gcggctgatc cagcccgagc atggtgcggg tagaggccag catctccggc gtcggcggca
    10561 ggttagataa acgcagataa tcgagcgccg ggtcgccggt accgaggcgc agcatcagaa
    10621 aaatgatcac cgaggcggcg agcaccatcg ggatcagcag cagaaagcgg cgtaatacgt
    10681 aacgcaacat taaggtttca ccggtttaat ctgttcgaac ggaatttcgg tggcgatcgg
    10741 cgcgtagggg atattaccca gctccggttt tgataccacc atcatcgaga tgtagctgat
    10801 aggcagataa accgcctcgt catgcagacg ggtcagaatg tcgcgataca gcgcctgacg
    10861 ttgcgtttcg tcatgggtcg ccagcacttc gccgatctct ttatcaatca gcggcttatc
    10921 ggctaatcct tgctgtgcct ggaagtcagc gtgcgacggt acgcgcattg aactcaggaa
    10981 ggcgtgtgga tcgtatggcg cgccccaggt gcggtggaaa atcatgccaa aacgaccgtc
    11041 gcgctgacga gcatagatac tgctctcttc ttcgccaatc agcgagacat ctgcgccaat
    11101 ctggcgcata tcagcctgaa tgatttccgc catcgatttg cttaacgcat cggtgccgat
    11161 gaacgacagt tcaatgcgca gcggctgacc atttttctcg cggatgtctt tgcccgcagg
    11221 cagcgtccaa ccggcttttt ccagcaacgc tttcgctttc tgcggatcgt actggcgcgg
    11281 tttcaggccg agattagcgt aaggcacaga aggggcaaac agggtgtcgg cgacctgctg
    11341 ggtgccatat aacgcgttat caatcagcga ttttttgttt accgcgtaat taagagcttc
    11401 acgtactgcc agctcgttgg tgggggcttt ggcggtattg agcgccagca tcacggtttc
    11461 gatcggctgt gacagttggg tgtggtaagc cggattctgg ctaaagcggg cgaaggtatc
    11521 gagcggtaat aacccttcgt ttccgtacag caggtcgata tcgccagttt caaacgccac
    11581 cgcgcgggta gtcgggtccg ggatgacgtt aaaggtgatc tttttaatcg ctggcttttc
    11641 gccccagtag ttttcgttac ggacgaagac atcgtactga ttcagtttcg attcctgcaa
    11701 aatccatggt ccggtgccaa tcggcgcttt aattccgttc atggtttcat ggtttttaaa
    11761 ctgcgaggga gcgataaaac ggaaaggacg gggcagggcc agttcttgca ggaaaggata
    11821 gtaggcgctt ttcagggtaa tttgcagctc tgttttactg agtgctttaa catcaacaat
    11881 ctggtttgcc agctccagcc aggcgtgacg ttgacggtta tcgagcactg cgcggaagtt
    11941 ttctgccgcc gcctcggcat cgaacggttc accgttggag aatttcacgt catcacgcag
    12001 ggtgaaggtc caggttttac catcttctga atgagtccag ctttttgcca gccacgggat
    12061 caccgaaccg tctgcctgat atttcaccaa tggttcataa accatgctct gggcgaacat
    12121 ctggttaggc gtgtaaaggt gcgggtttag tggcccgaca ttcaccggcc aggcggtagt
    12181 gatttcatct ggtgcagcgg catggacgat aaaagacgca caagccagca gcgcaaatag
    12241 agtgcggcgg agtgtggaga gcatggtaac cccaatggat taaaatagat ggcggaaata
    12301 agtatgacga ttttaagtat tcgtcatact gattacctgt tctggatcaa tagatgggca
    12361 gtcgggtggg cggatcagtt aactgaatcg atccattgca cactgtcggc ggtgagcgta
    12421 aagggagtag gggtgcagat cgccaggctt aaattttcaa gctgacaatg gctgactgac
    12481 agcgaggagt gataggtgct gtctacgctg acgatttgcc aggcgctgcc gccacgctgt
    12541 ttaacgatgg cttctttgcg cgtccagatg cgccagaaca tttccagttg ctgatcagga
    12601 tgcacggcgt ccatctcagc gtgttctccg aggctgaata cggcgttcgc cagccagcgc
    12661 cagttggcgc gcgggcgaat cacttcgata tcgcagccga cttcgccttc atcactcaac
    12721 agcagggcga tatcgtcacc gctatggctt aagttgaacc atagcggcat ttccggcgca
    12781 aatgcaggtt tgccttgttc gccatagatg atctccggta gcggggaaag cgtgtgcgaa
    12841 agcaatgcac gccccgccag ccagcgttcg cgtcgtggac cttgcggtgc ttgctcgcgt
    12901 aaacccggtg gcagtggagc tgcgcttaag gtcgaaactt tccccagaac tatccgatac
    12961 atatcagggc caacgtttaa tggaaaatga aagtgcgtat cgtatcactt gtcgcctcat
    13021 cccggtaacc gacttttcgg tctgcccggc cccagtaaaa tcgccagttt gctgccacct
    13081 ttcgtcgttt ccatccagat tttacacacg ctggttaatg gcaccgaaag tagcatccct
    13141 accgggccga gcagccatcc ccaaattaac aatgaaagaa ataccaccat ggtggacatc
    13201 cccaggcgat ggcccatcat ccgtggttct aaaatattgc cgatgaccat atggaccact
    13261 aaaaacaatg cgccgaccag aatacattcg taaacactat taaacagcag cacctgaatc
    13321 attggcggta cggcggaaat taccgcgccg atattgggca cgtagttgag caaaaacgcc
    13381 agtactgccc acatcagcgc aaactgcacc cccatcagct ccagccccag ccagacgatg
    13441 acacctgtcc ataaactgag tagcgtcttc aatgcaagat agtgcgaaac gcctttaagt
    13501 gcgcggtgta atcctgcgat gtgaatctgt gggttattca gcgcaaaacg cattttgtaa
    13561 gggacgtggc gcacttcaaa cagcataaaa actacggtca tcaccagcaa aagcacgctc
    13621 gccattgccc cggaaagccc ggtcattagc gctgtggtga aggtaaccac tttttccgag
    13681 tccatccgct gcagcattcg ctccggcgac atatgcaaat taagaaaagg caacatctcc
    13741 tgcaatttaa aaagtttgcg cgtcaactcc ttattaaact tcggcagcat agagataaat
    13801 tcgttaaacg atgccgccag tacgccgacc agcgcagtta gtgcgatcag catcaccacc
    13861 accacaatcg taatggcaat ggggcgttgt actccccgac gaataaacca ggtgacgagc
    13921 gggttgagga cgatggcaaa aaacagcgcc agtaacagct gcacaatgat atctgccgct
    13981 gcgtgaatgc ccgcgaggat cactaccagc gaggccagct tgagcagaat gtgcatgccc
    14041 gttttatcgg gttgaggggt ttccattggg gcttccttgt gactttttgt attaagtgta
    14101 gcgggagtca cgccagcaat attctgattc ttactgctga ttttcacgtc agcacgtggt
    14161 aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg cccgaacccg tagccgaacc
    14221 cgcgctaaac ggattgcgcc tgaatttgcg cattgtctct atagtcatgt ttaacttcgc
    14281 cagctacctc accatcgggt tgccgctcgc tgtattaccg ggctatgtcc atgatgtgat
    14341 gggctttagc gccttctggg caggattggt tatcagcctg caatatttcg ccaccttgct
    14401 gagccgccct catgccggac gttacgccga tttgctggga cccaaaaaga ttgtcgtctt
    14461 cggtttatgc ggctgctttt tgagcggtct ggggtatctg acggcaggat taaccgccag
    14521 tctgcctgtc atcagcctgt tattactttg cctggggcgc gtcatccttg ggattgggca
    14581 aagttttgcc ggaacgggat cgaccctatg gggcgttggc gtggttggct cgctgcatat
    14641 cgggcgggtg atttcgtgga acggcattgt cacttacggg gcgatggcga tgggtgcgcc
    14701 gttaggcgtc gtgttttatc actggggcgg cttgcaggcg ttagcgttaa tcattatggg
    14761 cgtggcgctg gtggccattt tgttggcgat cccgcgtccg acggtaaaag ccagtaaagg
    14821 caaaccgctg ccatttcgcg cggtgcttgg gcgcgtctgg ctgtacggta tggcgctggc
    14881 actggcttcc gccggatttg gcgtcatcgc cacctttatc acgctgtttt atgacgctaa
    14941 aggttgggac ggtgcggctt tcgcgctgac gctgtttagc tgtgcgtttg tcggtacgcg
    15001 tttgttattc cctaacggca ttaaccgtat cggtggctta aacgtagcga tgatttgctt
    15061 tagcgttgag ataatcggcc tgctactggt tggcgtggcg actatgccgt ggatggcgaa
    15121 aatcggcgtc ttactggcgg gggccgggtt ttcgctggtg ttcccggcat tgggtgtagt
    15181 ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg ctggcaactt acaccgtatt
    15241 tatggattta tcgcttggcg tgactggacc actggctggg ctggtgatga gctgggcggg
    15301 cgtaccggtg atttatctgg cggcggcggg actggtcaca atcgcgttat tactgacgtg
    15361 gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc gcctcatcat cttaaaactt
    15421 actgaataac cagcgtatta atgatgtttt ctgcggtggt ctgcgctttt tgctgatcgt
    15481 cagctggcag agtaatttgc atggtcagca gttgattacc cacgttaccc agaataacgg
    15541 aagagtacgc cgtctggcct ttcgcggaga taatactgtc taactgctgc attttgtgac
    15601 ctttcagctc aatggcttta ttggttacca cttgcagctg cggatcgcgg ctacgttgct
    15661 gatcttccag acgcttcgcc agcaccgcca gatcttcttt tggatcatcg cccatgatga
    15721 cgatgactgc tttctgcccg gtggcgtcgg accagacatg catgttattg gcctgcgttc
    15781 ccagcttacc gctctggtcg gtcatatccg ctggcagcga gaaacttaac ttgccatcaa
    15841 gcaggttgac gggattcccg gtagcgttac tttccgcgac cgaaccctgc gccgtagcgt
    15901 tagtgtcttt atcatcacag gccgcaagcc ccataaccag caggccaatt ccgacatatt
    15961 taaccagatt gcgcattgac ttcttccttt cgataaacgg ccataacggc tcattcatcc
    16021 atcttatcac aactctgata acgaaccttt aactcgcctg caaagcgttg atttcggatt
    16081 tatctgccag tcttttcaac agcatattga gtaatacgcc atacattggc aggaagaaaa
    16141 cgatactgat taacactttg aaacagtaat cgaccagcgc gatttccatc cagtgttcag
    16201 ccataaaggc atccgggcta cgccagaagg caatgaagaa aaaggccagc gtgtcgctga
    16261 cgttaccgaa cagtgtggac gctgtcggtg ccagccacca gcggcgactt tgacgcaggc
    16321 ggttaaaaac gtgcacgtcg aggatttgcc ccagcgcgta ggccatgaaa ctggcggtgg
    16381 cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc gaatccctgc catgaaccca
    16441 tatagaacag cgacgagatg acataggaga ttaataacgc agggatcatt accgcgaaga
    16501 taatgcgtcg ggccagcggt gcgccaaaaa tacgcacggt caggtcggtc gcgagaaaga
    16561 taaacggaaa actaaacgcg ccccaggtgg tatgaaaacc aaaaatggag accggaagct
    16621 ggaccagata gttactggag gtgatcacca gcagatgaaa taacgataac cagaacaacg
    16681 ccttatagcg ttgagtttgc gagaaaacgt tcatattgta cctttttgat taaccattgg
    16741 ggtgagggaa cccaatacgt acgacacgtc tgttatctta acataataac gtgcttaccc
    16801 ttttttcgag ccgccgcatg atactgcttt acgttgacaa tgcaatggtt aattttcacg
    16861 caatcgttaa cctggtttgc ttacggactc acagggcgta aactagcgcc gtttttttaa
    16921 gtgatgagaa gaaaatgacc gatctctttt ccagccctga ccacacactc gacgcgcttg
    16981 gcctgcgctg cccggaaccg gtgatgatgg tgcgcaaaac cgtgcgcaat atgcagcctg
    17041 gcgaaacttt actgattatc gccgacgatc cggcgacgac ccgcgatatt cctgggtttt
    17101 gtacctttat ggaacacgaa ctggttgcga aagagacgga tggactgcct tatcgttatt
    17161 tgattcgtaa aggcggttga tggggaaaga ttggcttcga tgccgccttt tcccctcacc
    17221 ctaaccctct ccccagagga gcgaggggac cgatcgcgct caatgttgcg atcggtttgc
    17281 cttatctcct gcgcaacaat cttaacgcat tcgctgttac cagcacggtc gcaccggtat
    17341 ctgccagcac cgccagccat agtccggtca tccctaacag cgtggtgacg aggaagatcc
    17401 ctttcagccc cagcgcaata gtgatgttct ggcggatatt ggcgtgagtg gcgcgtgcca
    17461 gttcaatcat ttgcaccagg ccgcgcaggt ggttatgggt taatgctgcg tcggcggttt
    17521 ccagcgccac gtctgtgcca ctgcccattg caatcccaat ggcggcagct ttcatcgctg
    17581 gcgcgtcgtt aataccatca ccgaccatcg ccaggggcgc atgttgattc agcttggtca
    17641 ccgctttgac tttatcttcc ggcaataggc ccgctttaaa ctccagcccc agctccccgg
    17701 caattgccgc cgctgcgcgt ggattatcgc cggtgaggat cactccttta acccccagcg
    17761 cgttcagttc actgatggca gttgctgcgt cggcgcgcag ggtatcctgc aatgcaatga
    17821 tacccagcac gtcatcatta cgtactacca gcacaaccgt ttgcccggcg ctttccagtt
    17881 cattaatcaa accagcaaat gcatcagcgg gatgtttccc ggcagcgcaa atcaatacgc
    17941 gctcaccgtt aacctgcgct tcaatgccag acccgaccag cgcccgctgt gattcggcgg
    18001 tgggaatggc gagttcagca acctgtgctt cgcgtacgat ggcctgcgcc agtggatgcg
    18061 tcgcgccttg ctcgaccgcc gccgccagtg tcagcagttc agattcacta atacccgttg
    18121 ccggatgaat cgcggtaacg cgcggtttac cgacggtcag cgtaccggtt ttatcaaacg
    18181 ccacctgagt aacacgaccc agctgttcca gcgccgctcc gcctttaatc aacgccccac
    18241 gacgcgctgc cgccgccagc ccggaggtaa tcgccgcagg cgttgagata actaacgcac
    18301 acgggcagcc aatcagcagc agcgtcagcc ctttataaat ccactcctgc cagctggcgg
    18361 caaacagcag cggtggcacc agcgtcacca gcagagcgac ggccataatc gcgggcgtat
    18421 agatacggct gaaacggtcg ataaaccgct caatgggtgc gcgacgctct tcggcttctt
    18481 cgatcagttt cagaatccgg tcaatggcgc tggcacccgg ttctgacagc acttccagcg
    18541 ttaccagacg gtctacgctg gtggctcctg caggaacttt atcgcccgtt gcgcgctcca
    18601 ccggaataga ttcgccggtc agggcgcttt catcaaaact ggcaaacggt gagagcagtt
    18661 taccgtcggc aggcaaacgt ccacctgcgg cgacttcaat cacatcgcca gggcgcaggc
    18721 tgttaatcgc cacctcttcc cgctcaccgt tacgcaggcg cgtggcggtt tctggtttca
    18781 gcgccattaa cgcgctgacc ccttgacgtg cgcggctggc ggcccagcct tccagtcgtt
    18841 cgccaatcaa aaacagcagc aacaccatcg cagcttcagc cgttgcgcca ataaacagcg
    18901 caccaatagc ggctacgctc attaacgttt caatggcgaa gtagctgccg gatttgatca
    18961 accgtaatgc ctgacgagca atcgggtaca gcccaaccag cgtggtcgcg ataaacgcca
    19021 gttgcccgaa cggatgattg aactgctcca gaccccagct gattgccatc atcacgatta
    19081 gcgtaatcag cgacagattc tctttcaggc gtgatgcttg cggttcttcg gcggcctgtt
    19141 catcgcgcag ggaatagcct gctttttgca ccgcagattc aacttgtgca cgaatgtcat
    19201 tgtcggcatc gaccaccagt ttttcggtgg cgaacaacac ctgcacctga ttcacgcctg
    19261 caagctggcg cacagcattt tctaccttgc gcgcacaggc ggcgcagtcc atgccgctga
    19321 ctttccagct atagcgggtg ccggagacgt tttcagagag cgttggcgag ctggaacatg
    19381 cgccgtcgca gcaacagtcg ttggcgttct gtaccgcagt tagcggtttg aacgcagcaa
    19441 attgaggggc tttcttgccg tgattgtcag gagtcgacat ggcatcctcc ggttaagttt
    19501 tttctcatta accgaaggat acactctgga gtcgactcca gagtcaagtt ttatcagaga
    19561 tacagcgagc ggacgatgag gaagtgaccg gcaaagtagc aggccgcagc aatcgcgtta
    19621 tccgcacgga agcgtcggcg atagtggctc cccagccaga caaagttact gataaacagc
    19681 aacgaagcgc caacaaacgc agagagcgcc ggagcggtcg gacggaagaa ccacagttca
    19741 cctgccagcc acaccatcac cagcgtcatg ccgataaacg tgcagatagg ccaacggtac
    19801 tcttccagcc gcgtccagat aatcgccagt agcagcgcac ccagaaccag caacaccagt
    19861 ggcagaggcc agaagaaaga gagcgtcatc tgactggcga aatagatggt gtacagcagg
    19921 tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt gacgtggcaa cagggttagc
    19981 gcatcgccca gcagtgaggc gcacagccct gccagcacca gatagctaat ggcgtcgaac
    20041 atcggcgctt gccaggccag taacagcagg agaagaaggg ttaacggttt aaacacccag
    20101 cgttgccagg ttggcccacg atacgatgca tccacagata gccatgcgga aagacagaca
    20161 gcgataaacg accaaagcat cttagttcct tgaattcgtt attttttcgt aagcggaatg
    20221 cctacggtct tgcttccagt ttagtggcga gggtggacgg atgacaacac cataactggc
    20281 agggtatgct tatttccgca ttttccgatg agggataaag atgagcaaac cacctctttt
    20341 ctttattgtt atcattggct taattgtcgt cgccgcatcg tttcgtttta tgcaacagcg
    20401 acgggaaaaa gctgataatg atatggctcc gctccagcaa aagctggtgg tggtgagcaa
    20461 caagcgggaa aaaccgatta acgatcgccg ttcgcgccag caggaagtga ctccggcagg
    20521 caccagtatg cgctatgagg caagcttcaa accgcaaagc ggaggaatgg agcagacgtt
    20581 tcgcctcgag gcccagcagt accacgccct gacagtgggc gataaaggta cgctgagcta
    20641 taaaggaacg cgctttgtca gctttgtagg cgaacaataa ggttattttt taactttaaa
    20701 tttcttctgc cagaccagca gttcaaacac gccgaaaagg aaaatccgca ccttttcggc
    20761 ggtggtcatc tgcgggccat ctttcggtaa agtggatttc aatagcgcca gttgcatacc
    20821 atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa atattcagcg ggcgcgggaa
    20881 gggatgcacc agattgagga ttaaaaatcc ccaaacgcag agcattaaca aacgaccaat
    20941 attaatcagc atcactttct ccttgtgctt cgcgttgata cagccgataa gccacctgac
    21001 ccgccacttt ttcccgatgt aatgaccagt ttgccggaac agtgggcaga ccgttttcga
    21061 cttcgctttc gacataaatc agggcttcgt cagccagcca gccgttatct tccagtaaat
    21121 ttatcgtctc ttctaacaag ccacggcgga acggtggatc gacaaacacg atattatgcg
    21181 gtgtgccttt ttgcgccagg aatgacattg cgttgctgtt caccacgcgt gcattgcctg
    21241 cttttagtgt cgccagattc ttaattaact gctgagaaac tgcgcgatcc atctcaatca
    21301 acgtggcccc cgcagcgtag cgcgataacg cttccagccc cagcgcgccg ctcccggcga
    21361 agcaatccag acattgggcg tcaacaatga ccggagccag ccagttaaac aacgtttcgc
    21421 gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg aaccgggagt ttacggcctc
    21481 gccactgccc gccaataatg cggatttggc cgctgccgga atgattcggt tttttcatga
    21541 taaattgctc aatccgccag ataacacata cttgcaggcg gtgatgtgaa ttaagttaag
    21601 tgatagacta tttcatcatt tttttagctg ctatgtacat agcgttaacg ctgtgccatg
    21661 aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa aaaaacgtgg ctttttttcc
    21721 tggctgggct ttggtcaaaa agagcagacc ccggaaaaag agacagaagt tcagaatgaa
    21781 caaccggttg tagaagaaat cgttcaggcg caagagcctg cgaaggcttc tgaacaagcc
    21841 gtcgaagagc aaccgcaggc gcatactgaa gccgaggcgg aaacttttgc tgccgacgtt
    21901 gtggaagtca ctgaacaggt tgctgaaagt gaaaaagcgc agcctgaagc ggaagtcgtt
    21961 gcacagccgg aaccggtcgt agaagaaacg gcggagccag tggctatcga acgtgaagag
    22021 ctgccgttgc cagaagacgt caacgccgaa gaggtttcgc cagaagagtg gcaggctgaa
    22081 gcggaaaccg tagagattgt cgaagcggca gaagaagaag cggctaaaga agaaatcacc
    22141 gacgaagagc tggaagcaca ggcgctggct gccgaagcgg cagaagaggc ggtgatggta
    22201 gttcctccgg cagaagaaga gcagccggtg gaagaaattg cccaggagca agaaaaaccg
    22261 accaaagaag gtttcttcgc gcgcctgaaa cgcagcctgt taaaaaccaa agaaaatctc
    22321 ggttccggat ttatcagcct gttccgcggt aaaaaaatcg acgatgatct gtttgaggag
    22381 ctggaagaac agctgttgat cgccgatgtg ggcgtggaaa ccacacgtaa aattatcacc
    22441 aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct ctatggcctg
    22501 ctgaaagaag agatgggcga gattctggcg aaagtcgatg agccgctgaa tgttgaaggt
    22561 aaaacgccgt tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac tacgacgatt
    22621 ggtaagctgg cacgtcagtt tgagcagcag ggtaaatcgg tgatgctggc ggcgggcgat
    22681 actttccgtg cagcagcggt tgaacagctt caggtctggg gtcagcgcaa caatattccg
    22741 gtgattgccc agcataccgg tgcggattcc gcctctgtta tcttcgacgc cattcaggca
    22801 gctaaagcgc gtaatatcga cgtcctgatt gccgataccg ccggacgtct gcagaacaaa
    22861 tcgcatctga tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa
    22921 gcgccgcatg aagttatgct gactattgat gccagcaccg ggcagaacgc ggtaagccag
    22981 gccaaactgt tccacgaagc tgttggctta accggcatca cgctaacgaa actggacggc
    23041 acggcgaaag gcggggtaat tttctcggtg gctgaccagt ttggtatccc tatccgctac
    23101 attggtgtcg gcgaacgtat tgaggatttg cgtccgttta aggcggacga ctttatagag
    23161 gcactttttg cccgagagga ttaacaatga ttcgctttga acatgtcagc aaggcttatc
    23221 tcggtgggag acaggcgctg cagggcgtta cgttccatat gcagccgggt gagatggcgt
    23281 ttctgaccgg tcattccggc gcagggaaaa gtaccctcct gaagctgatc tgtgggattg
    23341 agcggcccag cgccgggaaa atctggttta gcggccatga catcacgcgt ctgaaaaacc
    23401 gtgaagttcc gtttctgcgc cgccagattg gcatgatttt ccaggatcac catctactga
    23461 tggaccgtac tgtctacgat aacgtggcga tcccgctgat tatcgctggt gccagcggtg
    23521 acgatattcg tcgccgggtg tcggcggcac tggataaagt cgggctactg gacaaagcga
    23581 agaacttccc tattcagctt tccggcggtg aacaacagcg tgttggcatt gcccgcgcgg
    23641 tggtgaataa gcccgcggta ctgctggcgg acgaaccgac cggtaacctg gacgacgcgt
    23701 tgtcggaagg gattctacgt ctgtttgaag agtttaaccg cgttggggta accgtattga
    23761 tggcaacgca cgacatcaac ctgatttcac ggcgttccta tcgcatgctc accctgagcg
    23821 atggtcactt gcatggaggc gtgggccatg aataagcgcg atgcaatcaa tcatattcgg
    23881 cagtttggtg ggcgtctgga tcgcttccgt aaatcggtcg gcggctcagg cgacggcggt
    23941 cgtaacgcac cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt
    24001 ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc aggatctgaa aagcaaaccg
    24061 ttcgccacgt ttttaacggt gatggttatc gccatctccc tgacgctgcc cagcgtctgt
    24121 tatatggtgt acaaaaacgt taatcaggcg gcgacgcagt attatccgtc accgcaaatc
    24181 actgtttact tgcaaaaaac gctggacgat gacgccgctg cgggcgtggt ggcacagttg
    24241 caggccgagc aaggcgtgga gaaagtgaac tatctttctc gtgaagacgc actgggcgag
    24301 ttccgtaact ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa cccgcttccg
    24361 gcagtggcgg tggtgatccc gaaactcgat tttcagggga cggaatcact caatacgctg
    24421 cgtgatcgta tcacgcagat taacggcatt gacgaagtgc ggatggatga cagctggttt
    24481 gcccgtctgg cggcgttgac cgggctggtc gggcgcgttt cggcgatgat cggcgtgttg
    24541 atggtggcgg cagtgttcct cgtcatcggc aacagcgtac gtctgagcat ctttgctcgc
    24601 cgtgactcca tcaacgtgca gaaactgatt ggtgcgacgg atggtttcat cctgcgcccg
    24661 ttcctgtacg gtggcgcatt gctggggttt tctggcgcat tgttgtcact aattttgtca
    24721 gaaattctgg tactgcgatt gtcatcggcg gttgcggaag tggcacaggt tttcggaacg
    24781 aagtttgata tcaatggttt atcgttcgat gaatgcctgc tattgctgct ggtatgctcg
    24841 atgattggct gggtggcagc gtggcttgcc acggtacaac atttacgcca ctttacgcct
    24901 gaataataaa agcgtgttat actctttccc tgcaatgggt tccgtagcag ggaaagagac
    24961 cccgttgtct cttcccggta tttcatctct atgtcacatt ttgtgcgtaa tttattcaca
    25021 agcttgcatt gaacttgtgg ataaaatcac ggtctgataa aacagtgaat gataacctcg
    25081 ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga
    25141 gaggatttga atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga
    25201 ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct
    25261 ggctgaaaag ctgcattacc atggcgatct ggaagcagct aaaacgctga tcctgtctca
    25321 cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga
    25381 tttgattcag gaaggtaaca tcggcctgat gaaagcagtg cgccgtttca acccggaagt
    25441 gggtgtgcgc ctggtctcct tcgccgttca ctggatcaaa gcagagatcc acgaatacgt
    25501 tctgcgtaac tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt
    25561 caacctgcgt aaaaccaagc agcgtctggg ctggtttaac caggatgaag tcgaaatggt
    25621 ggcccgtgaa ctgggcgtaa ccagcaaaga cgttcgtgag atggaatcac gtatggcggc
    25681 acaggacatg acctttgacc tgtcttccga cgacgattcc gacagccagc cgatggcacc
    25741 ggtgctctat ctgcaggata aatcatctaa ctttgccgac ggcatcgaag atgataactg
    25801 ggaagagcag gcggcaaacc gtctgaccga cgcgatgcaa ggtctggacg agcgtagcca
    25861 ggacatcatc cgcgcgcgct ggctggacga agacaacaag tccacgttgc aggaactggc
    25921 tgaccgttac ggcgtttccg ctgagcgtgt gcgccagctg gaaaagaacg cgatgaaaaa
    25981 attgcgtgct gctatagaag cgtaatttcc gctattaagc agagaaccct ggatgcgagt
    26041 ccggggtttt tgttttttgg gcctctgtaa taatcaattt cccctccggc aaaacgccaa
    26101 tccccacgca gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatcggg
    26161 tatgttttag cagagtatgc tgctaaagca cgggtagtca tgcataaaac gaaataaagt
    26221 gctgaacaac aacatcacaa cacacgtaat aaccagaaga atggggattc tcaggatgaa
    26281 cataaagggt aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct
    26341 ggcagaagat attaaagtcg cggtcgtggg cgcaatgtcc ggtccggtgg cccagtacgg
    26401 tgaccaggag tttaccggcg cagagcaggc ggttgcggat atcaacgcta aaggcggcat
    26461 taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt
    26521 tgcggtggcg aacaaagtcg ttaacgacgg cattaaatac gtgattggtc acctctgttc
    26581 ttcatcaacg cagcctgcgt ctgacatcta cgaagacgaa ggcattttaa tgatcacccc
    26641 tgcggcaacc gcgccggagt tgaccgcgcg cggctatcag ctgatcctgc gcaccaccgg
    26701 cctggactcc gaccaggggc cgacggcggc gaaatatatt cttgagaaag tgaaaccgca
    26761 gcgtattgct atcgttcacg acaaacagca atacggcgaa ggtctggcgc gagcggtgca
    26821 ggacggcctg aagaaaggca atgctaacgt ggtgttcttt gatggtatca ccgccgggga
    26881 aaaagatttc tcaacgctgg tggcgcgtct gaaaaaagag aatatcgact tcgtttacta
    26941 cggcggttat cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg cagggctgaa
    27001 aactcagttt atggggccgg aaggtgtggc taacgtttcg ctgtctaaca ttgcgggcga
    27061 atcagcggaa gggctgctgg tgaccaagcc gaagaactat gatcaggttc cggcgaacaa
    27121 acccattgtt gacgccatca aagcgaaaaa acaggaccca agcggcgcat tcgtttggac
    27181 cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg atccggctga
    27241 aatcgccaaa tacctgaaag cgaactccgt ggataccgta atgggaccgc tgacgtggga
    27301 tgagaaaggc gatctgaaag gctttgagtt cggcgtattt gactggcacg ccaacggcac
    27361 ggcgaccgat gcgaagtaat cattaatcgg caactttggg ttgccgccaa attgctaata
    27421 tcgagtacgt tgcttcatgc cggatgcggc gtaaacgcct tatccggcct acaagatcca
    27481 aagaaatcag taaattgcaa cacacattgt aggcctgata agcgtagcgc atcaggcaat
    27541 ttctcgttgg tcatcacttt tgaaatcaac ttgacgatta acgcttctcc cagccgccct
    27601 gttgcgccgt aaatcccagt gcctgcataa acgccgtcat cacaccgcga tcttccacgc
    27661 ctgcatccgc catccaccag catgaaacgc caggattgtt acgcaaaacc tcttccagca
    27721 gatattgccc cacaccgcga cgtcgggtga cttcccgcac acgcagggaa tccagtgctc
    27781 cctcggtgcc gcttaaggtt acccgtacgg cagcgagcag gcgctcgtta aaacgcgcgg
    27841 cgtagatacg gtggttatcg tcaacctgta aagaggaagg ggaatactcc ggccagatct
    27901 tttgcaggtc aatccggtcc tggtcgctaa agttttctaa tcgaatgatg gtcagcttca
    27961 tgggtaaccc gtgtaaatca caaaagtgga accagtgtag cgaaataatt taatcggagg
    28021 ctttctcttt tttatttctt ttggcaggtg attaattttt taacagcaat aattacaaag
    28081 ttaaaacatt agagaatgaa aaatacccag cataatcccc tgaatggtag tgaattattc
    28141 cgccctttgt gccgttattt tatgctgaca aaggcacttt tttctgttta tctatcaata
    28201 aattcagaat attatctgtt cttaatcgac tgaaaaatag ggattttaat cactattatc
    28261 acaaaatact gcgctaaacc cttaatcaga caggcaaaaa cactgcagta taaaaaaaga
    28321 acaggctgat ttgttaacac ataaaaacaa agcaacacaa catcacgaat ggggattttt
    28381 gactatgaaa cggaatgcga aaactatcat cgcagggatg attgcactgg caatttcaca
    28441 caccgctatg gctgacgata ttaaagtcgc cgttgtcggc gcgatgtccg gcccgattgc
    28501 ccagtggggc gatatggaat ttaacggcgc gcgtcaggcg attaaagaca ttaatgccaa
    28561 agggggaatt aagggcgaca agctggttgg cgtggaatat gacgacgcct gcgacccgaa
    28621 acaagccgtt gcggtcgcca acaaaatcgt taatgacggc attaaatacg ttattggtca
    28681 tctgtgttct tcttctaccc agcctgcgtc agatatctat gaagacgaag gtattctgat
    28741 gatctcgccg ggagcgacca acccggagct gacccaacgc ggttatcaac acattatgcg
    28801 tactgccggg ctggactctt cccaggggcc aacggcggca aaatacattc ttgagacggt
    28861 gaagccccag cgcatcgcca tcattcacga caaacaacaa tatggcgaag ggctggcacg
    28921 ttcggtgcag gacgggctga aagctgctaa cgccaatgtt gttttcttcg acggcattac
    28981 cgctggcgag aaagatttct ccgcgctgat cgcccgcctg aaaaaagaaa acatcgactt
    29041 cgtttactac ggcggttact acccggaaat ggggcagatg ctgcgccagg cccgttccgt
    29101 tggcctgaaa acccagttta tggggccgga aggtgtgggt aatgcgtcgc tgtcgaatat
    29161 tgctggcgat gctgccgaag gcatgttggt caccatgcca aaacgctatg accaggatcc
    29221 ggcaaaccag ggcatcgttg atgcgctgaa agcagacaag aaagatccgt ccgggcctta
    29281 tgtctggatc acttacgcgg cggtgcaatc tctggcaaca gcccttgagc gtaccggcag
    29341 cgatgagccg ctggcgctgg tgaaagattt aaaagcaaac ggtgcaaaca ccgtgattgg
    29401 gccgctgaac tgggatgaaa aaggcgatct taagggattt gattttggtg tcttccagtg
    29461 gcacgccgac ggttcatcca cggcagccaa gtgatcatcc caccgcccgt gaaaaacggg
    29521 cgggtttaga aaggttacct tatgtctgag cagtttttgt atttcttgca gcagatgttt
    29581 aacggcgtca cgctgggcag tacctacgcg ctgatagcca tcggctacac catggtttac
    29641 ggcattatcg gcatgatcaa cttcgcccac ggcgaggttt atatgattgg cagctacgtc
    29701 tcatttatga tcatcgccgc gctgatgatg atgggcattg ataccggctg gctgctggtg
    29761 gcggcgggat tcgtcggcgc aatcgtcatt gccagcgcct acggctggag tatcgaacgg
    29821 gtggcttacc gcccggtgcg taattctaag cgcctgattg cgcttatttc cgcaatcggt
    29881 atgtccatct tcctgcaaaa ctacgtcagc ctgaccgaag gttcgcgcga tgtggcgctg
    29941 cccagcctgt ttaacggtca gtgggtggtg ggccatagcg aaaacttctc tgcctctatt
    30001 accaccatgc aggcggtgat ctggattgtc accttcctcg ccatgctggc gctgacgatt
    30061 ttcattcgct attcccgtat ggggcgcgcc tgtcgtgcct gcgcggaaga tctgaaaatg
    30121 gcgagcctgc ttggcatcaa caccgaccgg gtgattgctt tgacctttgt tattggcgcg
    30181 gcgatggcgg cagtggctgg cgtactgctc ggtcagttct acggcgtcat taacccctat
    30241 atcggcttta tggccgggat gaaagccttt accgctgcgg tgctcggtgg gattggcagc
    30301 attccgggag cgatgattgg cgggctgatt ctggggattg cggaggcgct ctcttctgcc
    30361 tatctgagta cggaatataa agatgtggtc tcattcgccc tgctgattct ggtgctgctg
    30421 gtgatgccga ccggtattct gggtcgcccg gaggtagaga aagtatgaaa ccgatgcata
    30481 ttgcaatggc gttgctctct gccgcgatgt tttttgtcct ggcgggcgtc tttatgggcg
    30541 tacaactgga gctggatggc accaaactgg tggtcgacac ggcttcagac atccgttggc
    30601 agtgggtgtt tatcggcacg gcggtggtct ttttctttca gcttttgcga cctgctttcc
    30661 agaaagggct gaaaagcgtt tccggaccga agtttattct gcctgcaatt gatggctcca
    30721 cggtgaagca aaagctgttc ctcgtggcgc tgctggtgct tgcggtggcg tggccgttta
    30781 tggtgtcgcg cgggacggtg gatatcgcca ccctgaccat gatctacatt attctcggtc
    30841 tcgggctgaa cgtggttgtt ggtctttctg gtctgctggt gctggggtac ggcggctttt
    30901 acgccatcgg cgcttacact tttgcgctgc tcaatcacta ttacggcctg ggcttctgga
    30961 cctgtctgcc gattgccggg ttaatggccg cggcggcggg cttcctgctc ggctttccgg
    31021 tgcttcgctt gcgcggtgac tatctggcga tcgttaccct cggtttcggc gaaattgtgc
    31081 gcatattgct gctcaataac accgaaatta ccggcggccc gaacggaatc agtcagatcc
    31141 cgaaaccgac tttcttcggc cttgagttca gccgtaccgc ccgtgaaggc ggttgggaca
    31201 cgttcagtaa tttctttggc ctgaaatacg atccctccga tcgcgtcatc ttcctgtatc
    31261 tggtggcgtt gctgctggtg gtactaagcc tgtttgtcat taaccgcctg ctgcggatgc
    31321 cgctggggcg tgcgtgggaa gcgttgcgtg aagatgagat cgcctgccgt tcgctgggct
    31381 taagcccgcg tcgtatcaag ctgaccgctt ttaccatcag tgccgcgttt gccggttttg
    31441 ccggaacgct gtttgctgcg cgccagggct ttgtcagccc ggaatccttc acctttgctg
    31501 aatcggcgtt tgtactggcg atagtggtgc tcggcggtat gggatcgcag tttgcggtga
    31561 ttctggcggc aattttgctg gtggtatcgc gcgaattgat gcgtgatttc aacgaataca
    31621 gcatgttaat gctcggtggt ttgatggtgc tgatgatgat ctggcgtccg cagggcttgc
    31681 tgcccatgac gcgcccgcaa ctgaagctga aaaacggcgc agcgaaagga gagcaggcat
    31741 gagtcagcca ttattatctg tgaacggcct gatgatgcgc ttcggcggcc tgctggcggt
    31801 gaacaacgtc aatcttgaac tgtacccgca ggagatcgtc tcgttaatcg gccctaacgg
    31861 tgccggaaaa accacggttt ttaactgtct gaccggattc tacaaaccca ccggcggcac
    31921 cattttactg cgcaatcagc acctggaagg tttaccgggg cagcaaatcg cccgcatggg
    31981 cgtggtgcgc accttccagc atgtgcgtct gttccgtgaa atgacggtga ttgaaaacct
    32041 gctggtggcg cagcatcaac aactgaaaac cgggctgttc tctggcctgt tgaaaacgcc
    32101 ttctttccgt cgcgcccaga gcgaagcgct cgaccgcgcc gccacctggc ttgagcgcat
    32161 tggtttgctg gaacacgcca accgtcaggc gagtaacctg gcctatggtg accaacgccg
    32221 tctggagatt gcccgctgca tggtgacgca gccggagatt ttaatgctcg acgaaccggc
    32281 ggcaggtctt aacccaaaag aaaccaaaga gctggatgag ctgattgccg aactgcgcaa
    32341 tcatcacaac accactatct tgttgattga acacgatatg aagctggtga tggggatttc
    32401 agaccgaatt tatgtggtca atcaggggac gccactggca aacggtacgc cggagcaaat
    32461 ccgtaataac ccggacgtga tccgtgccta tttaggtgag gcataagatg gaaaaagtca
    32521 tgttgtcctt tgacaaagtc agcgcccact acggcaaaat ccaggcgctg catgaggtga
    32581 gcctgcatat caatcagggc gagattgtca ccctcattgg cgcgaacggg gcggggaaaa
    32641 ccaccttgct cggcacgtta tgcggcgatc cgcgcgctac cagcgggcga attgtgtttg
    32701 atgataaaga cattaccgac tggcagacgg cgaaaatcat gcgcgaagcg gtagcgattg
    32761 tcccggaagg gcgtcgcgtc ttctcgcgga tgacggtgga agagaacctg gcgatgggcg
    32821 gcttttttgc cgaacgcgac cagttccagg agcgcataaa gtgggtgtat gaactgtttc
    32881 cacgtctgca tgagcgccgt gttcagcggg caggcaccat gtccggcggt gagcaacaaa
    32941 tgctggcgat tggtcgcgcg ctgatgagca acccgcgttt gctgctactt gatgagccat
    33001 cgctcggtct tgcgccgatt atcatccagc aaattttcga caccatcgag cagctgcgcg
    33061 agcaggggag gactatcttc ctcgtcgagc agaacgccaa ccaggcgcta aagctggcgg
    33121 atcgcggcta cgtgctggaa aacggccatg tggtgctctc cgataccggc gacgcgctgc
    33181 tggcgaacga agcagtgaga agtgcgtatt taggcgggta ataacacgtt gattgataag
    33241 gagtcaaaag actcctttga gacaggtgac aaatgtaaaa ttgcctgatg cgctgcgctt
    33301 atcaggccta ctgggtgagt tgcaatatat tgaatttgca cgatcttgta ggccggataa
    33361 ggcgtttacg ccgcatccgg catgaaacga tgagtaatct gtagagtttg attcagacct
    33421 tctatttttc cgcttatccg tgccccatct cccattttcc ctcacccacg ccgtcaccgc
    33481 cttgtcatct ttctgacacc ttactatctt acaaatgtaa caaaaaagtt atttttctgt
    33541 aattcgagca tgtcatgtta ccccgcgagc ataaaacgcg tgaattcgcg cattcggtac
    33601 aacaagagag ataaacgatg aaatcgttac attatacagc ttcagcactg gcgctcggac
    33661 tggcgttaat ggggaatgca caggcagtga cgaccattcc gttctggcat tctatggaag
    33721 gggaactggg taaagaggtg gattctctgg cccaacgttt taacgccgaa aatccggatt
    33781 acaaaattgt accgacctat aaaggcaact acgaacagaa tttaagcgcg gggattgccg
    33841 catttcgtac cggcaacgct ccggctattt tgcaggttta tgaagttggc accgccacca
    33901 tgatggcatc gaaagccatt aaaccggtat atgacgtgtt taaagaggcg gggattcaat
    33961 tcgatgagtc gcagtttgtg ccgacggttt ccggttacta ctccgacagc aaaacgggcc
    34021 acttactctc ccagccattc aacagctcga cccccgttct ctattacaac aaagacgcct
    34081 tcaagaaagc aggattagac ccggaacaac cgccgaaaac ctggcaggat ctggcggact
    34141 atgccgcgaa actgaaagcc tccggtatga agtgcggcta cgccagcggc tggcagggct
    34201 ggatccaact ggaaaacttt agcgcctgga acggtctgcc gtttgccagc aaaaacaacg
    34261 gctttgacgg cacggacgcg gtgctggagt tcaacaagcc ggagcaggtg aaacacatcg
    34321 ccatgctcga ggagatgaac aagaagggcg acttcagcta cgtcggtcgt aaggatgaat
    34381 ccaccgagaa gttctataac ggtgattgcg cgatgaccac cgcctcttcc ggttctcttg
    34441 ccaacattcg cgagtacgcc aaatttaatt acggcgtagg catgatgcct tacgacgccg
    34501 atgcgaaaga tgcgccacaa aacgccatta tcggcggagc cagcctgtgg gtgatgcagg
    34561 gtaaagataa agaaacgtat accggtgtgg cgaagttcct cgatttcctc gcgaagccag
    34621 aaaacgctgc cgagtggcat cagaaaaccg gttatctgcc aattaccaaa gcggcgtatg
    34681 acctgacccg tgagcagggc ttttacgaga aaaacccagg tgcggatacc gcgacgcgtc
    34741 agatgctgaa taagccgccg ttgccgttca ccaaagggct gcgtctgggc aacatgccgc
    34801 agatccgcgt gattgtggat gaagagctgg agagcgtgtg gaccggtaag aagacaccac
    34861 agcaggcact ggataccgcc gttgagcgtg gaaatcagtt gctgcgccgc tttgagaaat
    34921 cgacgaagtc ttaatcagtg taatgtcgga tgcgtttcgc ttatctgacc tggcatcgcg
    34981 tgtaggccgg ataagcgaag cgcatccggc acagttcagg aattaaccgt aatgtcatca
    35041 tcccgtccgg tgttccgctc gcgctggctg ccttatctgc tggtcgcgcc gcagctcatc
    35101 atcaccgtta tcttttttat ctggcctgcg ggcgaagcgt tgtggtactc gctacaaagc
    35161 gtcgatccgt ttggtttctc cagccagttt gtcggcctgg ataacttcgt cgcgctgttt
    35221 catgacagct actatctcga ctccttctgg acgacgataa aattcagcac ctttgtcacc
    35281 gtcagcggtt tgctggtgtc gctgttcttc gcggcgctgg tggagtacat cgtgcgcggc
    35341 agccgtttct atcaaacctt aatgctgctg ccttacgccg tggctcccgc cgttgccgcc
    35401 gtattgtgga tcttcctgtt taaccccggt cgcgggctga tcacccattt tctcgcggag
    35461 ttcggctacg actggaacca cgcgcaaaac agcggtcagg caatgtttct ggtggtgttt
    35521 gcctcagtat ggaagcaaat cagctacaac ttcctgttct tctatgccgc gctgcaatcc
    35581 attccccgtt cgttgatcga agccgcagcc atcgacggtg caggcccaat tcgccgcttc
    35641 tttaagattg cgctgccgct tatcgctccg gtgagtttct tcctgctggt ggtgaacctg
    35701 gtgtatgcct tcttcgacac cttcccggtg atcgacgccg ccacgtccgg cgggccggtt
    35761 caggccacca cgacgctgat ttataagatc taccgtgaag gctttaccgg actggatctg
    35821 gcttcgtctg ccgcgcagtc ggtggtgttg atgttcctcg tcatcgtgct gacggtggtg
    35881 cagttccgct atgttgaaag caaggtgcgt taccaatgat tgagaaccgt ccgtggctga
    35941 cgatattcag ccataccatg ctgatcctcg ggatcgcggt gatcctcttc ccgctgtacg
    36001 tggcgtttgt cgcggcgacg ctggataaac aggccgtcta tgccgcgccg atgacgctca
    36061 tccccggcac acatctgctg gaaaatatcc acaacatctg ggttaacggg gtaggcacga
    36121 atagcgcgcc gttctggcgg atgttgctta acagctttgt gatggcgttc agcattacgc
    36181 tcggcaaaat taccgtctcg atgctctcgg catttgccat tgtctggttt cgttttccgc
    36241 tacgtaacct cttcttctgg atgattttta tcaccctgat gctgccggtt gaagtacgta
    36301 tcttcccgac ggtggaagtc atcgccaacc tgaagatgct cgacagctac gccggtttaa
    36361 cgctgccgct gatggcctcg gcgaccgcca cgtttctgtt tcgccagttc tttatgacgc
    36421 tgccggatga actggtggaa gcggcgcgga tcgacggtgc gtcaccaatg cgcttctttt
    36481 gcgacatcgt ttttccgctc tccaaaacta atctggcggc gctgtttgtg atcaccttta
    36541 tttacggctg gaaccagtat ttgtggccgt tgttgattat taccgacgtg gatctcggca
    36601 ccaccgtggc agggatcaaa gggatgatcg ccactggcga aggcaccacg gaatggaact
    36661 cagtgatggc ggcgatgttg ttaaccctga tcccaccagt agtgattgtt ttagtgatgc
    36721 agcgtgcctt cgtgcgcggc ctggtcgata gtgagaaata agatggcagg actgaaatta
    36781 caggcagtaa ccaaaagctg ggatggcaaa acccaggtca ttaaaccgct gacccttgat
    36841 gtggcagatg gcgaatttat cgtgatggtc gggccgtctg gctgcgggaa atcgacgctg
    36901 ctgcgcatgg ttgccgggct ggagcgggtg accgaaggcg atatctggat caacgaccag
    36961 cgcgtgaccg aaatggagcc gaaagatcgc gggattgcga tggtgttcca gaactacgcg
    37021 ctttatccgc atatgagtgt tgaagaaaac atggcgtggg ggctgaaaat tcgcggcatg
    37081 ggcaagcagc aaattgccga gcgcgttaaa gaagcggcgc gcattctgga actggacggt
    37141 ctgcttaagc gtcgcccgcg tgagctatcc ggcggtcagc gccagcgtgt ggcgatgggc
    37201 cgcgcgattg tgcgcgatcc ggcggtgttc ttgtttgatg agccgctctc taacctcgat
    37261 gccaagctgc gcgtgcagat gcgtcttgaa ctgcaacagt tgcaccgtcg cctgaaaacg
    37321 acttcactct acgttactca cgatcaggtt gaagcgatga cgctcgccca gcgagtaatg
    37381 gtgatgaacg gcggcgttgc cgaacagatt ggcacaccag ttgaagtcta cgaaaagccc
    37441 gccagcctgt ttgtggcgag ttttatcggc agcccggcga tgaacctgct gacaggccgc
    37501 gtgaataatg aaggcacgca tttcgaactg gacggcggta tcgtgctacc gctaaacggt
    37561 ggctaccgtc agtatgccgg gcgtaaaatg actctcggca ttcgcccgga acatatagcg
    37621 ctaagctcgc aggcagaagg tggtgtgccg ctggtgatgg acacgctgga gatcctcggc
    37681 gcagataacc tggcgcacgg acgctggggc gagcagaagc tggtggtgcg actggcgcat
    37741 caggagcgcc cgacggcagg cagcacgctg tggctgcatt tggcggaaaa tcagctgcat
    37801 ctttttgatg gtgaaacagg acaacgagta tgagtaactg gccttatccc cgcatcgtcg
    37861 ctcatcgtgg cggcggtaag ctggccccgg aaaacaccct ggcggcaatc gacgtcgggg
    37921 caaaatacgg tcataagatg atcgaatttg acgcgaagtt atcgaaagat ggcgagatct
    37981 tcctgctcca tgacgacaat ctcgaacgca ccagcaacgg ctggggcgtc gcgggtgaac
    38041 tgaactggca ggatttactg cgcgtggatg cgggcagttg gtacagcaaa gcgtttaaag
    38101 gtgagccgct gccgttgctt tcgcaggtgg cggaacgctg tcgcgaacac gggatgatgg
    38161 cgaatatcga aatcaaaccc accactggca ctggaccatt aacgggcaaa atggtggcgc
    38221 tggcggcacg ccaactgtgg gccggtatga cgccgccgct gctgtcatcg tttgagattg
    38281 atgctttaga agctgcacaa caggcggcac cggaactgcc gcgtggttta ttgcttgatg
    38341 agtggcgcga cgactggcgc gaactgaccg cgcggctggg ctgcgtctct attcatctca
    38401 atcataagtt actcgataaa gcgcgagtga tgcagttgaa agacgccgga ttacggattc
    38461 tggtttatac cgtcaataaa ccccagcgcg cggcagagtt gctgcgctgg ggcgtggatt
    38521 gcatctgtac cgatgcgatt gacttgattg gtccgaactt tacggcccaa tagttttcaa
    38581 cggaatatca ggctgcggcg tgctgggctg attcagcatg tcgccgtttc tcctttccgg
    38641 cagcatatgc tgctgattca acgaactatc cggattacgg ttgctgttta acatcccgcc
    38701 gttggtgttg ggcagcattt gctgccgggc gggatttcgc tcccccggct gtgactgcaa
    38761 cacccgctga gaattattgt ttatctggtt ttctaaatgc tgctgttgca actgagtttg
    38821 cgttttcagt tgctgattca gcatcccttt ttgctggatt tgctgagtct gcatctgggt
    38881 ttgcatccgc tgctggctgg gtatctgata ccccggctgg ttagggttgt tcagagtatt
    38941 aatgggctgt gcaaagccga caaacggcag gagtgccgta agaatcagaa gtcgtttcat
    39001 cgcgtatcct cctctgaaga tatcctttaa gtttactcgc ttcccgacaa aacgatgatt
    39061 aattcagagt tatataccgg gcttagctgg ggttgcccct taatctctgg agaataacga
    39121 tgataaaacc gacgttttta cgccgggtgg ccattgctgc tctgctctca ggaagttgtt
    39181 ttagcgccgc cgccgcgcct cctgcgccgc ccgtctcgta tggtgtggag gaagatgtct
    39241 tccacccggt acgcgcgaaa cagggaatgg tagcgtctgt ggacgccact gccactcagg
    39301 tgggggtgga tattctcaag gagggcggga atgccgttga tgccgccgtg gcggtgggct
    39361 acgcgctggc ggtaacgcat ccgcaggcag ggaatctggg cggtggtggt tttatgttaa
    39421 tccgctcgaa aaatggcaat accaccgcta tcgatttccg cgaaatggca cccgccaaag
    39481 cgacccgcga tatgttcctc gatgatcagg gcaacccgga cagcaaaaaa tcactcactt
    39541 cgcatctggc ttccggcaca ccgggtacgg tagcaggttt ctcgctggcg ctggataaat
    39601 acggcaccat gccgctgaac aaagtcgtgc agcccgcgtt taaactggca cgcgatggtt
    39661 ttatcgttaa cgacgcgctg gctgacgatc tcaaaaccta cggtagcgaa gtgttgccga
    39721 atcacgaaaa cagtaaagct atcttctgga aagagggcga gccgctgaaa aagggcgaca
    39781 cgctggtgca ggcgaacctg gcaaagagcc tggagatgat tgctgaaaac ggcccggacg
    39841 aattctataa aggcacgatt gcggaacaga tcgcccagga gatgcagaaa aacggtggct
    39901 tgatcactaa agaagattta gcggcctata aagcggtcga acgcactccg ataagcggcg
    39961 attatcgcgg gtatcaggtt tactccatgc caccgccatc ctccggcggg atccatatcg
    40021 tacaaatcct caatattctg gaaaacttcg atatgaagaa atacggcttt ggcagcgccg
    40081 atgcgatgca aatcatggca gaagcggaga aatacgccta cgccgaccgc tcggaatatc
    40141 ttggcgaccc ggattttgtc aaagtcccgt ggcaggcact gaccaataaa gcctatgcca
    40201 aatccattgc cgatcaaatt gatatcaaca aagcgaagcc gtccagtgag attcgccctg
    40261 gcaagcttgc gccttatgag agtaatcaaa ctacccatta ctctgtagtg gataaagacg
    40321 gtaacgcggt ggcggtgacc tatacgctga acaccacctt cggtacgggt attgtcgcgg
    40381 gcgagagcgg tattctgctt aataaccaga tggatgattt ctccgccaaa ccgggcgtac
    40441 cgaacgttta cgggctggtg ggcggtgatg ccaacgccgt cgggccgaac aaacgcccgc
    40501 tgtcgtcgat gtcgccgacc attgtggtga aagacggtaa aacctggctg gttaccggta
    40561 gcccaggcgg tagccggatc atcactacag tgctgcaaat ggtggtgaat agcatcgatt
    40621 atggcatgaa cgtcgccgaa gcgaccaatg cgccgcgttt ccaccatcag tggttgccgg
    40681 acgagctgcg tgtcgaaaaa gggtttagcc cggatacgct caagctgctg gaagcaaaag
    40741 gtcagaaagt ggcgctgaaa gaggcgatgg gcagtacaca aagcattatg gttgggccgg
    40801 acggtgagtt gtacggcgca tccgacccgc gctcggtgga tgatttaacg gcggggtact
    40861 aaggttagcg gccctcttcg tgggaagagg gctattttgt cagggcaagc cgaaggtagc
    40921 cttttttatt tcgtaatcct gtagatattc ttccagcggc tttgccgttc ccagagaatg
    40981 aatttcacca ctgtctttat caataataaa aggtgcgtta ccagctaagc gcgcggcctc
    41041 atctccagtt tcgagaaatt ctcgtgcttc gaaacagaaa taccagccct ggctaaagcg
    41101 tccatgtaga gtaatgacga ccgggagatc tgcatcatca aggtaatggt tcgctttcgc
    41161 gaatgcgtcg tgataagtaa tcataattat aataaatatt attgttgagt gttatattat
    41221 tattcctgtg tgatacattg agcaaagacg cgttcatctt cgtcaaagat attattaaat
    41281 gctggttttg aaaaaagttc atcggataag atatagtcac gaaataagta tctgctaata
    41341 ttttatgatt tctttttcag aacgtcgaac gggattttct tatttaagat atcagaatct
    41401 tatgattcgg gtaaaattct aaataaatca acatgtcatt aaaatcatca tagccattag
    41461 ctatttcggc taaaatagag actacatgtc ttcggtccat ctcacttaag gagtgtagtt
    41521 ccgttgtaag tttttccata gcttgcactg ctaaatttcg aacaaggaat tttctgctgg
    41581 taatctctaa aaagatggca tggtttacaa tgatttttgt ttccttttga ttattatgaa
    41641 caactgtcca tgatttcgtt taagaatgaa gagaaatcac taaacgaact gaatatattt
    41701 tctgtgccaa tattatctct aatttcaaaa aagttacttt taatgtcata agtaaa
//
LOCUS       DAZKIV010000022        41140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZKIV010000022 DAZKIV010000000
VERSION     DAZKIV010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.022"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..1464)
                     /gene="ccp"
                     /locus_tag="ACHOT6_001247"
     CDS             complement(67..1464)
                     /gene="ccp"
                     /locus_tag="ACHOT6_001247"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFP9946496.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1869..3518
                     /locus_tag="ACHOT6_001248"
     CDS             1869..3518
                     /locus_tag="ACHOT6_001248"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9946497.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3569..4171)
                     /locus_tag="ACHOT6_001249"
     CDS             complement(3569..4171)
                     /locus_tag="ACHOT6_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HFP9946498.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            4690..5589
                     /locus_tag="ACHOT6_001250"
     CDS             4690..5589
                     /locus_tag="ACHOT6_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9946499.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDPPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            5638..6651
                     /gene="yhjD"
                     /locus_tag="ACHOT6_001251"
     CDS             5638..6651
                     /gene="yhjD"
                     /locus_tag="ACHOT6_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFP9946500.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPRK
                     TQP"
     gene            7062..8384
                     /locus_tag="ACHOT6_001252"
     CDS             7062..8384
                     /locus_tag="ACHOT6_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946501.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(8618..10678)
                     /locus_tag="ACHOT6_001253"
     CDS             complement(8618..10678)
                     /locus_tag="ACHOT6_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9946502.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDVNAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVYSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(10748..11515)
                     /gene="pdeH"
                     /locus_tag="ACHOT6_001254"
     CDS             complement(10748..11515)
                     /gene="pdeH"
                     /locus_tag="ACHOT6_001254"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFP9946503.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            11747..12676
                     /gene="kdgK"
                     /locus_tag="ACHOT6_001255"
     CDS             11747..12676
                     /gene="kdgK"
                     /locus_tag="ACHOT6_001255"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFP9946504.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(12772..14268)
                     /locus_tag="ACHOT6_001256"
     CDS             complement(12772..14268)
                     /locus_tag="ACHOT6_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9946505.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLTESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINRAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(14489..15775)
                     /gene="dctA"
                     /locus_tag="ACHOT6_001257"
     CDS             complement(14489..15775)
                     /gene="dctA"
                     /locus_tag="ACHOT6_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFP9946506.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            complement(15958..17946)
                     /gene="hmsP"
                     /locus_tag="ACHOT6_001258"
     CDS             complement(15958..17946)
                     /gene="hmsP"
                     /locus_tag="ACHOT6_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFP9946507.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            complement(18028..21501)
                     /gene="bcsC"
                     /locus_tag="ACHOT6_001259"
     CDS             complement(18028..21501)
                     /gene="bcsC"
                     /locus_tag="ACHOT6_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFP9946508.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTLEPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPTQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            complement(21483..22589)
                     /gene="bcsZ"
                     /locus_tag="ACHOT6_001260"
     CDS             complement(21483..22589)
                     /gene="bcsZ"
                     /locus_tag="ACHOT6_001260"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFP9946509.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            complement(22596..24926)
                     /gene="bcsB"
                     /locus_tag="ACHOT6_001261"
     CDS             complement(22596..24926)
                     /gene="bcsB"
                     /locus_tag="ACHOT6_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFP9946510.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERVWYAL
                     ANHPILLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            complement(24937..27555)
                     /gene="bcsA"
                     /locus_tag="ACHOT6_001262"
     CDS             complement(24937..27555)
                     /gene="bcsA"
                     /locus_tag="ACHOT6_001262"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFP9946511.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLMGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            complement(27552..28304)
                     /gene="bcsQ"
                     /locus_tag="ACHOT6_001263"
                     /pseudo
     CDS             complement(27552..28304)
                     /gene="bcsQ"
                     /locus_tag="ACHOT6_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
     gene            complement(28316..28504)
                     /gene="bcsR"
                     /locus_tag="ACHOT6_001264"
     CDS             complement(28316..28504)
                     /gene="bcsR"
                     /locus_tag="ACHOT6_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFP9946512.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            28777..30348
                     /gene="bcsE"
                     /locus_tag="ACHOT6_001265"
     CDS             28777..30348
                     /gene="bcsE"
                     /locus_tag="ACHOT6_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFP9946513.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAATAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRRLDDAVERSS"
     gene            30345..30536
                     /gene="bcsF"
                     /locus_tag="ACHOT6_001266"
     CDS             30345..30536
                     /gene="bcsF"
                     /locus_tag="ACHOT6_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFP9946514.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            30533..32212
                     /gene="bcsG"
                     /locus_tag="ACHOT6_001267"
     CDS             30533..32212
                     /gene="bcsG"
                     /locus_tag="ACHOT6_001267"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFP9946515.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISNLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGNWVPYPQ"
     gene            complement(32299..32406)
                     /locus_tag="ACHOT6_001268"
     CDS             complement(32299..32406)
                     /locus_tag="ACHOT6_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9946516.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(32782..32889)
                     /locus_tag="ACHOT6_001269"
     CDS             complement(32782..32889)
                     /locus_tag="ACHOT6_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9946517.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            33365..34636
                     /locus_tag="ACHOT6_001270"
     CDS             33365..34636
                     /locus_tag="ACHOT6_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFP9946518.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(34666..35670)
                     /gene="dppF"
                     /locus_tag="ACHOT6_001271"
     CDS             complement(34666..35670)
                     /gene="dppF"
                     /locus_tag="ACHOT6_001271"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFP9946519.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSFSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(35667..36650)
                     /gene="dppD"
                     /locus_tag="ACHOT6_001272"
     CDS             complement(35667..36650)
                     /gene="dppD"
                     /locus_tag="ACHOT6_001272"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFP9946520.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(36661..37563)
                     /gene="dppC"
                     /locus_tag="ACHOT6_001273"
     CDS             complement(36661..37563)
                     /gene="dppC"
                     /locus_tag="ACHOT6_001273"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFP9946521.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(37573..38592)
                     /gene="dppB"
                     /locus_tag="ACHOT6_001274"
     CDS             complement(37573..38592)
                     /gene="dppB"
                     /locus_tag="ACHOT6_001274"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFP9946522.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(38900..40507)
                     /gene="dppA"
                     /locus_tag="ACHOT6_001275"
     CDS             complement(38900..40507)
                     /gene="dppA"
                     /locus_tag="ACHOT6_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFP9946523.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(40859..>41140)
                     /locus_tag="ACHOT6_001276"
     CDS             complement(40859..>41140)
                     /locus_tag="ACHOT6_001276"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946524.1"
                     /translation="SVASGNGTALIVGCGATALFDLHTTHFPAKFAPHISLSINGFVT
                     ETIVFFYICLNLFLRYLWLTLAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         9634 a        11263 c        10375 g         9868 t
ORIGIN      
        1 ttaaaaacag actttattat cgtaataaaa atcgcccaaa cattcctgtt cgggcgatca
       61 aattaattat tgtttatcct gcatatacgg cgtgtacacc ccgttcagac tgtgcaggaa
      121 agctacgata tcatccacat cctcctgcgg cagctctttg cctacctgat agcgcagcat
      181 cagtttcacc gccccgtcca gcgtcggcac gtcaccgcga tggaagtacg gtgccgttaa
      241 agcaacgtta cgtaaaccgg gtactttctg acgcaattta tcacgctctt ctttagtcac
      301 attcatacga ccaatatccg ccgccgtaat ttccccaaag ttaaagtctt ttttcagccc
      361 caacggttca aaggaacgtc cgccgagaat aataccacca tgacaagttg cacatttatt
      421 atctttaaat aattgatagc cttttttctg ttgcgccgtc agcgcatttt catctccacg
      481 caaccattta tcaaatgggg aatccggcgt aattaatgtt ttctcaaatt cagcaatggc
      541 atcagtaata ttttcgccac tgaaaccttg cggataaact tcgaggaact gcgctttaag
      601 ctgcggatct ttttccagct tagcaataat ttcgtcccag gatttcgacg ccatttcaat
      661 cgggttcaac ggcggtccac ctgcctgatc ctgcaatgtt gccgcacgac catcccagaa
      721 ctgctcaacg ttaaatactg agttaaatac cgtcggcgcg ttaatcggcc caactgcgcc
      781 accaacacca atcgatgttt ttctgccatc gacgcccccc gcattcaacg catggcaatg
      841 agcgcatgaa atggtgctat cagccgataa acggggatcg tgatacagcg caaagcccaa
      901 cgccactttt tgcgcatcgg taggcagttt ttgcgggatg ggctgcaccg gttcattgcg
      961 atgctccgga gcagtatcat tgctggcgta atattccgcg cgctgttttg caatccaggc
     1021 cagtatttcc gcccgctctt catcactcac cttacccgcc cagtgtagcg cggtataacg
     1081 cgttggtggc atagtttcat actgcatcac ccattcaatc ttattcagat cgctttgcga
     1141 aacgggttta tcagccagca gtgccgcacg cacggcctcg aggttaaaag atttatatcc
     1201 aagcttaatg tcgtaatcca tcaactgttt cgcgccagga atataataat aggcgggtaa
     1261 ttctgccgaa ggcgtgtggc aatagtcgca tcctttttcg cggagaaagc ctaaaacctt
     1321 attattttca ctgacagcag atgcctgaac atcggcctgt ttactgcgtt tattatcgtg
     1381 gtaccacaca taaccagata accctaaata gcaaatcgcg acgccagcca ggccgatcgc
     1441 ggtaatacgt gagaccattt tcattattct tccctcacgg tggtgagtta ttgtgaccca
     1501 gaaaattttc cagaatgctg caaacgtctc tgcgagacaa agccatcata aaaaacaggg
     1561 agatacgctt tttgatagga atcaatcaat tacatgaata taaactatca ataagatagc
     1621 ctgtgcctat tacccagatc aggaatggca tatcatgatt gcggcgagag caggattgaa
     1681 tgttctgaaa atgaaaaaca acacgaaatc cgtattttta taaattccat ggatattagt
     1741 gcgttatatt ttttgacagg ttaataaacg tgatccaccg cacgctttgt cgcccaccag
     1801 gcggagcgaa tgactaccct taaagaaaag cccgataatt agcgacgaat ttcggaggtt
     1861 ggatccttat gctcaatcag aaaattcaaa accctaatcc agacgaactg atgatcgaag
     1921 tcgatctctg ctatgagctg gacccgtatg aattaaaact ggatgagatg atcgaggcag
     1981 aaccggaacc cgagatgatt gaagggctgc ccgcctctga tgcgctgacg cctgccgatc
     2041 gctatctcga actgttcgag catgttcagt cggcgaaaat tttccccgac agtaaaacct
     2101 ttcccgactg cgcacccaaa atggacccgc tggatatttt aatccgctac cgtaaagtgc
     2161 gccgtcatcg tgattttgac ttgcgcaagt ttgttgaaaa tcacttctgg ctgccggagg
     2221 tctactccag cgagtatgta tcggacccgc aaaattccct gaaagagcat atcgaccagc
     2281 tgtggccggt gctaacccgc gaaccacagg atcacattcc gtggtcttct ctgctggcgc
     2341 taccgcagtc atatattgtc ccgggcggcc gttttagcga aacctactat tgggattcct
     2401 atttcaccat gctggggctg gcggaaagtg gtcgggaaga tttgctgaaa tgcatggccg
     2461 ataacttcgc ctggatgatc gaaaactacg gtcacatccc caacggcaac cgcacctatt
     2521 atttgagccg ctcgcaacca ccggtttttg cgctgatggt ggagttgttt gaagaagatg
     2581 gtgtacgcgg tgcgcgccgc tatctcgacc accttaaaat ggaatatgcc ttctggatgg
     2641 acggtgcaga atcgttgatc cctaatcagg cctatcgcca tgttgtgcgg atgccggacg
     2701 gatcgctgct caaccgttac tgggacgatc gcgacacgcc gcgtgacgaa tcctggcttg
     2761 aggacgttga aaccgcgaaa cattctggtc gcccgcccaa cgaggtatac cgcgatttac
     2821 gcgcgggggc ggcctccggt tgggattact cttcccgttg gctgcgtgat actggtcgtc
     2881 tggcgagcat tcgtaccacc cagttcatcc ccatcgatct gaatgccttc ctgtttaaac
     2941 tggagagcgc catcgccaac atctcggctc tgaaaggtga gaaagagaca gaagcactgt
     3001 tccgccagaa ggccagtgcc cgtcgcgatg cggtaaaccg ttacctctgg gatgatgaaa
     3061 acggcatcta ccgcgattac gactggcgac gcgaacaact ggcgctgttt tccgctgccg
     3121 ccattgtgcc actctatgtc ggtatggcga accatgaaca ggccgatcgt ctggcaaacg
     3181 ccgtgcgcag tcggttactg acacctggcg ggattctggc aagcgagtac gaaaccggtg
     3241 aacagtggga taaacccaac ggctgggcac cgttacagtg gatggcaatt cagggattta
     3301 aaatgtatgg cgatgacctt ctgggtgatg aaatcgcgcg aagctggctg aagacggtga
     3361 atcagttcta tctggaacag cacaaactga tcgaaaaata ccatattgcc gatggtgttc
     3421 cccgcgaagg cggcggtggc gagtatccgt tgcaggatgg gtttggctgg actaacggtg
     3481 tggtacgccg tttaattggt ttgtacggcg aaccataata tttttacagc cagccgctaa
     3541 cttcctgctg gccgtaaaat tatcctcttc aggaggagat atttaacatc attgccgcct
     3601 gggtgcgatt tttcacttcc agacggcgat acagggattc cagatgcgct tttaccgttc
     3661 cggtactgat attcagcgct ctgccgatct ccttatttga ttcgcccgcc gctaacatgg
     3721 ttaaaatctc ccgctggcgg gcgcttaacg atttgagatc tttaatgtcc ttttccggcg
     3781 tcgtccgcca gtctccaggc agaaacatca tccccatcgc cgcactattt accgccaacg
     3841 caaatgtctc gacggttgaa tcacgaggca caatggccag cacattaaaa tgaataactt
     3901 cctgtaacca ccgtttattg caatccgtcg ccgtgattaa caccttaacc tcaggaaact
     3961 gcaccacggt tttttgcagc aaccagtagc aaaactcacc atcctgatcg ccatcgagca
     4021 taactaaggc ttcagggtaa ctttccagct tttgccataa ctcgtctgcc tgactggccc
     4081 cctgaatact cactcctgga atacgctgct gtaaactgat tttcattcca tgaataaata
     4141 ttgactgcct gtcaaacatg actatttgca taactgaatc tccacctgaa tacgttaaaa
     4201 agacttaagt agtggaaggg tattacccgc gagaaaaaat aagaattcgc catttggcgg
     4261 tggccattct acagagatga cgcgtagaaa atagttaccg atataaatag ttacagctaa
     4321 acgcctgaaa ttacatgtcg agggcactat ttaaaacaat tttgaggatt tccttatatt
     4381 gatggttagt acgcatgcaa ttaaaaatga aattccgcga ccacaagcca aaataacaaa
     4441 cggcaaggag acaaaaataa gcacaaatag ccaatacatc ctctattcac tttaaaggga
     4501 atcgctgaaa aatacgctct gtttaagggg attcaccttc tcataaagct attccgccct
     4561 tttcctgctg agaaatcgcc acattcgtca tgacaacatt gtgaaacccg gcattagatg
     4621 ttagaaaaaa caataaacaa tgcgatatgc gcgttactgt ttcgctgtga acaacaatcg
     4681 gtcaaagaaa tggataaaat tcacgcaatg cagttgttca tcaaagtcgc ggagctggaa
     4741 agtttttccc gcgcggcgga tttctttgct ttgccaaaag gaagtgtttc gcgccagata
     4801 caggcactgg aacatcaact tggcacccag cttctccagc gcaccacgcg acgggtcaaa
     4861 ctcacgccag aaggcatgac ctattatcaa cgagcaaaag atgtgttgag taatctcaac
     4921 gaactggacg gtctgtttca gcaggatgcc accagtatca gcggtaaatt acgcatcgac
     4981 cccccgccag gaatcgcgaa aagcctgtta ctgccgcgcc tgtcggaatt tctctatctg
     5041 catccgggga ttgagctgga actgagtagc catgaccgtc cggtagatat tcttcatgat
     5101 ggttttgatt gcgtgatacg cactggcgcg ttaccggaag atggcgttat cgcccgtccc
     5161 ctcggcaaac tgaccatggt caactgtgcc agtccgcact atctgacgcg ctttggttat
     5221 ccgcaaagcc ccgatgatct gacttcacac gcaatagtgc gttatacacc gcacctgggt
     5281 gtacatccgt taggttttga ggttgccagc gttaatggcg tccagtggtt taagtctggc
     5341 ggcatgttga cggtaaacag tagcgaaaac tatctcgccg ccggtcttgc cggtctgggg
     5401 attattcaga tcccgcgcat tgccgtgcgc gaagccctgc gtgccgggcg ccttattgaa
     5461 gtgttgccag gctaccgtgc cgagccgctc tccctttcgc tggtttatcc gcagcgtcgg
     5521 gagctttccc ggcgtgtaaa cctgtttatg cagtggctgg ctggcgtaat gaaagagtac
     5581 ctggactgac cgactatact ttttaagaat gaccacgaca gaaggacaaa agagcggatg
     5641 acgcaggaaa acgagatcaa acgtcccacc caggatctgg agcacgagcc gattaagcag
     5701 ttggataata gcgagaaagg cggcaaagtt agccaggcgc tggaaaccgt caccaccact
     5761 gccgaaaaag tccaacgcca accggttatt gcgcacctga ttcgcgcgac agaacgcttt
     5821 aacgatcggc tgggtaacca gtttggtgcg gctatcacct atttctcatt tttatcgatg
     5881 atcccgattt tgatggtgtc gtttgccgca gggggctttg tgctggcctc ccatccgatg
     5941 ttgctacagg atatcttcga caaaattctg caaaacatca gcgatccgac gctagccgcc
     6001 acgttgaaaa acaccatcaa caccgccgtt cagcagcgta cgactgtagg gcttgtcggc
     6061 ctggcggtgg cgctttattc tggcatcaac tggatgggta acctgcgtga agcgattcgt
     6121 gcccagtcgc gcgatgtctg ggaacgctcg ccgcaagatc aggagaagtt ctgggtaaaa
     6181 tatctgcgtg attttatttc actgattggt ttgttaattg cgctgattgt gacgctgtcg
     6241 atcacctcgg ttgccggttc ggcgcagcaa atgattatta gcgccctgca cctgaacagc
     6301 attgagtggc tgaaaccgac gtggcggttg attggcctgg cgatttccat cttcgccaac
     6361 tatctgcttt tcttctggat cttctggcgg ttgccgcgcc accgtccgcg caaaaaagcc
     6421 ctgattcgcg gaacatttct ggctgctatt ggttttgaag tgattaaaat cgtgatgacc
     6481 tacaccttgc catcgctgat gaaatccccc tctggcgcag catttggttc cgtgctgggg
     6541 ctgatggcat ttttctactt cttcgcccgt ttgacgctgt tttgcgcggc gtggattgcc
     6601 accgccgaat ataaagacga cccgcgaatg ccaaggaaaa cgcagcctta aaataggccg
     6661 gatgcggcgt aaacgcctta tccggcatac gattttattc ggcctggctc cccgtaggcc
     6721 ggataagatg cgccagcatc gcatccggct ataatgcgca cataacctct tgaaactctt
     6781 ccccagagcc tcttcagcca tctatttggg agcaaacaat ttcattccaa ctcataaccc
     6841 cagcatataa atccagttgg taacttttat ttaacctgaa accagtttta tccactattt
     6901 ataaaattat gtgaagcatt tcatagaaga aaaatcactg gcctaaacat tatccccttt
     6961 ttgcctggtt tttgaccatt tccgcgattt gttacacatt gaaatatcac ttttgctgtg
     7021 cgtaatatgg ctattcgtta gccaaaaaat aagaaaagat tatgcaagca acagccacaa
     7081 cactcgacca cgagcaagaa tacacgccga tcaactcgcg taataaagtc cttgtcgcct
     7141 ctctcattgg cacggccatt gagttcttcg acttttacat ttacgccact gcggccgtta
     7201 ttgtgtttcc gcatatcttc ttcccgcagg gcgatccaac ggcagcaacg ctacagtcgc
     7261 tcgccacctt cgccatcgcc ttcgtcgcgc gccccattgg ctctgccgtt tttggtcatt
     7321 ttggcgatcg cgttgggcgt aaagcgacgc tggtcgcctc gttgctaacg atggggattt
     7381 cgaccgtggt gattggtctg ctgccgggct atgccacgat tggtattttc gccccgctgc
     7441 tgctggcgct ggctcgattt ggtcagggtc tgggcttagg cggtgaatgg ggcggcgcgg
     7501 cgctgctggc gactgaaaac gccccaccgc gcaaacgtgc actgtatggc tccttcccac
     7561 agctgggcgc accgattggc ttcttctttg ccaacggcac tttcttgctg ctttcctggc
     7621 tactgaccga cgagcagttt atgagctggg gctggcgtgt gccatttatc ttctcggcgg
     7681 tgctggtcat tatcggcctg tatgttcgcg tgtcgctgca tgagtcgccg gtgtttgaga
     7741 aagtcgctaa agcgaaaaaa caggtgaaga tcccgctggg tacgctgctg accaaacatg
     7801 ttcgcgtaac cgtactgggt acgttcatta tgctggcaac ctatacgctg ttttacatca
     7861 tgacggtcta ctctatgacc tttagtaccg ccgccgcgcc agttgggctt ggcctgccgc
     7921 gtaacgaagt gttgtggatg ctgatgatgg cagttattgg ttttggcgtg atggtgccag
     7981 tcgccggatt actggcagat gcctttggtc gccgtaaaag catggtaatt atcaccacgc
     8041 tgatcatcct gttcgcgctg ttcgccttta acccgctgct tggttctggc aacccgattc
     8101 tggtttttgc cttcctgctg ctggggttaa gtctgatggg gctgaccttc gggccgatgg
     8161 gcgcgctgtt accagagctg ttcccaacag aagtgcgtta caccggagca tcgttctctt
     8221 acaacgtagc gtcgattctc ggggcttccg ttgcaccata tatcgcagcc tggttgcagg
     8281 ctaactacgg gttaggtgcg gtggggttat atctggcggc gatggctggc ttgacgttaa
     8341 tcgccctgct gctgacccat gagacgcgac atcagtcgtt gtaagtatcg tgtgggcagc
     8401 caccctcacc ccagccctct ccctgaaagg gagagggaga aaagcggagc gagatgttat
     8461 atcgcgaaca taacagccct cccccagaaa gtgaacggaa aagcggtact cgttgtttca
     8521 acacgcacaa aacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacacgc
     8581 tgctcccgaa ctcaccagca aacacaatcc aacctcatca cttcttcatc tgcgacaata
     8641 tcgtccgaca ctgattagcc tccccttcgg aaggtgagat cagtgccagt aacgccgccg
     8701 ccggtgttac cagcgttgcc agtgccgcag caacagcacc acgggcaatc aacggcccgg
     8761 ctttcacccc agcctgcgga tttttaaacg tcccacgcac atacagcggc gaacgcagtg
     8821 tgataatccg aatcccttta ctctccggat caatagtcaa atccagctgt tccgaagcaa
     8881 aacttgccgt gccggtaaca ttaatcaacg cgttctcagt atcgaaagca aaaatctgcg
     8941 ggcgcgccac gccgttggca atattcagat tcgccgccgc gcagttcacc cgcacctcat
     9001 cgtcaccaaa tatcgcaccg acaatgtagt tgccgacatt cagcccaaca atctccatca
     9061 ggttgcggct caccagcccg tcattcatca acagtttcag gttgccgtta ctgttgccta
     9121 aaagtgctgc caccgagtta ccgctaccgc gtagttccgc gtcaccgttc atttccccca
     9181 gcgtcttctg catcagttcc acatcgggca tcagttcttt cagtttcagt cgacgagcct
     9241 gaatatctgc ccgcccctgc atcggctttt tatcgccttc cagatgaata ttcgccgcaa
     9301 tgctgccgcc cgccatgcca aatttcagcg gttgcaggcg caggtcagca tttttgagga
     9361 tgatatgagt agaaagatcg ctaatcggca ggctactgcc atgctcaatg cgccgccctt
     9421 tgaagcgaac atcggcatcc ataacgtccc atttatcggt ttcgaagcgg tcataaggca
     9481 gcaccttgcc cgcaggctga acgctttttt cgcccttctt ctgttcagac cgtttcgact
     9541 tttctgcccc tttcccggag taaacgccaa ttaacggtcc caggtccgcc agccgcaatt
     9601 gccgcgactc gacatcacct tccagttttg gtcgtggctt tccggtggtg tagaccagag
     9661 aaccgtggat atcgctatcg ccaattcgcc cattaaaacc gcgataatca aagaccgacg
     9721 atttttcagt gtcgattttc gctaccagcc gaccatccgt ttcaaacggc ggggtatcgg
     9781 gcagcagaac gcccgtcagt tcatagagat cacccagtga atcgccagaa aatttaagcc
     9841 gtaaatcgac accgcccatc ttcattgggt cattcacgac gccatcaaaa gcaacacggg
     9901 tgttaccaga gcggaaatca gcctgtaccg gaaacggcgt cccttcgcca cgcagcgcca
     9961 gcataccgcc tatttttccc gtacccgtga gcggttcacc gttataacgt ccctgcgcct
    10021 tcaggccaaa aacgtaatcg cccacctttt ctttatccgc tttacctttc gatccagtaa
    10081 cttcgctgaa cggcagcggc ttgcctaacg gatcaacaaa aatctccaga tccgctttgc
    10141 ttactttgtc atcaatggcg atccgccctt gatcgaaaag aatattatcc agccgaaacg
    10201 accatgccga cggctttgca ttcacgtctt tgttatcatc gttggcgaga ttaaacgtcc
    10261 agttattgtt cttttcggag aggcgaatca ggcgcgcgtc gggcttttcg agcttgatcc
    10321 acggcagcca gaccgttttg gtcagcagcg ccagcggggc cagcgttgcc tctacgcgtg
    10381 gcaaatgcac catcgtgact tcgggaatat ccggtggatt gccaagaatg atgtcttccg
    10441 cgtgtacatg gggccacggc acccagctgc gccagccagt ttcttgtttt tgccgctccc
    10501 acaccacgcc cagatcgcca cggatagcga acggacgatt caactccgca gagactttct
    10561 ggttgatggt cggtttgagt cgattccagt caaaagttgc aatcaaaatg atcgccacga
    10621 caatcaacaa caagaaagcc actgaaatcg cagcggttat tttgcctgcc ttgctcatcg
    10681 ttcgcttcct catattcttc ctgtgccagt cctaaagata gtccagccag gcggaaaatg
    10741 aggcagctta tagcgccaga accgccgtat tcagcgtttc tatcggtgcc gggcgtgaaa
    10801 gaaaccagcc ttgtgcggcg aatgcgggcg agttctgaac atcacgccac tcttccggcg
    10861 tttctacgcc ctcgacaatc accccgcgac aatagcgatt cattagatgt aaaagctgag
    10921 aaaagagtgt gcgtccttcc ggcgactgac gcagcatcac aaacagttct cgcgcgattt
    10981 tgatgtagtc ataacgcact tcacttagtg cagagaaatt tgccatcccg gtaccaaaat
    11041 catccagcca cagcgggcca aattcacaca tcgaggcaaa ggttgaatct ttcggcagac
    11101 ggatatgctc caccagttcg aaacgcagcc agggaagacg ctcaatctgg cgcaggattt
    11161 ttggttgctg acgcagggcg atgagcgtag ggccatcaat attgaccgat gccagcaggc
    11221 cgtgctctat aaagaagtcg gctttttgcg ccagcaaatc aatctgctct ttcacaacct
    11281 ccatccgatg gctgacggtg atttcagtaa aatagcgatc cggcggcagg cgttgcgaag
    11341 ggttcaaggg atgcgtgacc accgttaata gctccacggc cattaaccgt ccgcatgttt
    11401 gatagatcgg ctgccaggtg taagcacgct cacactgcaa ccaaaaacgc cgttcctgca
    11461 agctctcgat gcttgcttca gggttgctta ttcgctggat aacctgcctt atcatcaaag
    11521 atgtcctgtg tttaaaagtg atgctgcccg gactcgacaa agattatcgg cgcgcaaggg
    11581 cagaacttta ccgcctggcc ggggtaaatt tcctcactcc acaacggaat ctttcaggaa
    11641 tacgttctgg ctcaaaaaaa taatggaaca ctgttttaat atggttgacc agcaaaccac
    11701 cacagcgcaa actaacgcta attttttaca gatcaggttc acgactatgt ccaaaaagat
    11761 tgccgtgatt ggcgaatgca tgattgagct ttccgagaaa ggcgcggacg ttaagcgcgg
    11821 tttcggcggc gataccctga acacttccgt ctatatcgcc cgtcaggtcg atcctgcggc
    11881 attaaccgtt cattacgtaa cggcgctggg aacggacagt tttagccagc aaatgctgga
    11941 cgcctggcac ggcgagaacg ttgatacttc cctgacccaa cggatggaaa accgtctgcc
    12001 gggcctttac tacattgaaa ccgacagcac cggcgagcgt acgttctact actggcggaa
    12061 cgaagccgcc gccaaattct ggctggagag tgagcagtct gcggcgattt gcgaagagct
    12121 ggcgaatttc gattatctct acctgagcgg gattagcctg gcgatcttaa gcccgaccag
    12181 ccgcgaaaag ctgctttccc tgctgcgcga atgccgcgcc aacggcggaa aagtgatttt
    12241 cgacaataac tatcgtccgc gcctgtgggc cagcaaagaa gagacacagc aggtgtacca
    12301 acaaatgctg gaatgcacgg atatcgcctt cctgacgctg gacgatgaag acgcgctgtg
    12361 gggtcaacag ccggtggaag acgtcattgc gcgcacccat aacgcgggcg tgaaagaagt
    12421 ggtggtgaaa cgcggggcgg attcttgcct ggtgtccatt gctggcgaag ggttagtgga
    12481 tgttccggcg gtgaaactgc cgaaagaaaa agtgatcgat accaccgcag ctggcgactc
    12541 tttcagcgcc ggttatctgg cggtacgttt gacgggcggc agtgcggaaa acgcggcgaa
    12601 acgcgggcac ctgaccgcaa gtaccgttat tcagtatcgc ggcgcgatta tcccgcgtga
    12661 ggcgatgcca gcgtaaacgc agcatatgca aaaggctgga taaagtgtta cccctctatc
    12721 cagctcatcc ataaatacct gatggttgtg ctaactcatc accatcaggc attactgtgc
    12781 aggtggaata tccgtcactt caggatgtac gtcatccgtg gcgacagagg tggcggcagc
    12841 agcagtcgat ggggccatga tttgatccca ggccgcctgc aacgctttca tgttaaattc
    12901 cggctcgcct ttcggctgca gcagtattaa cgccatatca ttcgacaact gctgacgcag
    12961 atcctgattt aacatctcca ccgtcaggct gttaaggaaa tcctgccgca gtttctgata
    13021 ctgctccggc gcgatatcga cgacctgatt ttgcaacgaa cgcatccgct gacccatcag
    13081 aatatcggta tcagcccgcg cataggcggc aaacagtttc tgcagctcca gtttcttttg
    13141 cgccactaac gcattgaact cttcttccgg cagaccttta tcgcgaacct tcgccagttc
    13201 acgcgccacc agattaaggt tgctgttcag cttgtcgttt ggtgattcga tgttgatggc
    13261 acactgcgca cgcagataca gcacacggca gtcaaatcca agaccgatgt ctttgctgtt
    13321 actggcgctt aacgcttgct gaacatgcca gaacagcgcc tcgcgggcca ggtccgcacg
    13381 ccagtagcgc agcagtgcgg ctgattcacg aatcggctgc cacggcgtat cccacatgat
    13441 agataaccgg tcctgacgca ccgcgtcagt cataatactc accgcttccg cacgcagcgg
    13501 agaaagcgtc ggcaccggag ctggcgtttc acgtttgcct ttcagttcgc caaacgtttt
    13561 gtttatttgg tcgacaaccg agcgcgcatc cacgtttccc accaccagta gcgtcattgc
    13621 atccggggtg taccatttct gatagaaatc tttaattttt tccgcttcaa cgggttgttt
    13681 cagcggatcg gcaggatcgt gacctaacaa ggttgaccct ttcagacgat agcgccacca
    13741 gccctcttta gtatcggcag gccaggttgc caccatgtcc tgactttgca gcgcgcggtt
    13801 gatggtttct ggtgtgatgg tcaatttgcc agtggcattt gccagataag agagcgcttc
    13861 tttcagcaaa tcgttacggt tattgggcaa actcagatta aacagcgtgg tgtcataaga
    13921 gacaattacc ggcggcatcg ggcgtttagg gtcgatcccc tgctgccaca atgaacgcgc
    13981 ctgtgctgcg tcaaggccac cgctttgcgt tagcgcaata cgagggatgg cgtgactgta
    14041 accgctctgt tgtgtacttt cggtgagcga accggtatta accagcaggc gaatttcaac
    14101 acgatcgctg ggacgctggg gggtggtcag cacttgccac tgtaaaccgt tggaaagcgt
    14161 cccctgttgc catgctggat caggctggag cgcatctgcc tgcacatagc cagcagtggc
    14221 catcatcagc aaaccgcccg ctaaaagtcg aatttttgtg ccctgcatgt gaacccctga
    14281 tcaactatcc tggtaataaa aaaactgccc gttgtcggtc agtctaaatg acgttaaaaa
    14341 cacttcgtgt tagaccgcaa gaacatgaaa atgtcacgga agaagtgaaa taaacctgaa
    14401 ctcacccagg tggtaagttc aggcacaggg gtcaattatg cgcaaacacc cgcactcggg
    14461 gaagggagtg cgggcataag tggtgagatt aagaggataa ttcgtgcgtt ttgccatccg
    14521 gcgcacgatt attcagcaca tcgtccagtt ttttgtggtc cagttctttc acccacttag
    14581 caacgacaat ggtcgctacg ccgttaccga ccaggttagt cagcgcacga gcttctgaca
    14641 taaagcggtc gataccgagg atcagcgcca gacccgctac cggcaaatgg cccaccgcag
    14701 agagcgtcgc cgccagcacg ataaagccac tacccgttac ccctgccgcc cctttagaag
    14761 aaagcagcaa cacgattaac agcgtgattt ggtggacgat atccatctga ctgttagtgg
    14821 cctgggcgat aaacaccgcc gccattgtca ggtatatcga tgtgccatca aggttaaacg
    14881 agtagcctgt cgggatgacc agccccacca ccgatttacg gcagccgagt ttctccatct
    14941 tgtcgagcat acgcggcagc gccgactcgg aagatgaagt ccccagtaca atcagcagtt
    15001 cttcacggat gtagcggata aatttgaaga tactgaaacc agtcgcttta gcgattgaac
    15061 ccaataccag caccacaaac aggatacagg taatgtagaa acagataatc agctgcccca
    15121 gttgcaccag tgtgccgacg ccgtatttac cgatggtaaa cgccattgcc ccgaacgcac
    15181 caataggtgc cagacgcatg atcatattga tgatgccgaa gatgacctgc gagaaacttt
    15241 cgatcacgtt aaaaatcagt tggcctttgc tgcccagacg gtggagcgca aaaccaaaca
    15301 gtacggcaaa cagcagcacc tgcagaatgt taccgctggc aaatgcgcca atgacgctcg
    15361 ccgggatgac atccataatg aaggcgacaa tgccctggtc tttcgcctga tcggcgtaaa
    15421 ccgctaccgc tttcgcatca agcgttgccg gatcgacgtt cattccggca ccaggctgca
    15481 cgacgttaac gatgataaga ccaataatca gcgcgatggt actgacaatt tcaaagtaaa
    15541 gcagtgcgac tgcgccggta cgaccgaccg ccttcatgct ttccatgccc gcaatgcccg
    15601 ttacgacggt acaaaagatg acaggagcga tgatcatctt aatgagctta acgaacccgt
    15661 cgccaagcgg tttcatttgc tcgcctattt caggatagaa atggccaagg agaataccaa
    15721 tggctatcgc tgtcaggacc tgaaagtaaa ggcttttaaa cagagaggtt ttcatagggt
    15781 gtcctttagt aaaaccacag gtcttgtaag gttatggggt acctgcggcc ttaaaataac
    15841 acccagacaa catcacagaa atgtacctgg atcataattg aaacaaaaag gttaaaaagt
    15901 ttgagctggc tcgcacaaac cagcactttt taaagttttg taatcagttt ggggtagcta
    15961 cttttcttcc aggtaactct cttcgaagat ttcgataggg agtgggcgag caaaaaggaa
    16021 gccctgggca ataccaacgc ccgcttttgc cagccagtcg cgttgtgctt cagtctccac
    16081 gccttcggca atcatttgta agttcaggct ctgcgccagc atgataattg cagcaatcat
    16141 gctgctatct tccggcaagc cttcaacaaa cattttgtcg attttcagta cgtcgattgg
    16201 caacgatttc atatgctgca gctgacgcag ccctgcgtag cccatgccga aatcatccag
    16261 cgccacccga acgccggcat tgcgcagcgg acggaggatt gccaccgcag catgagggtc
    16321 gtcaatacgt cggctttctg tcacttccag aatcagtgtt cccggctgaa tgcgatagcg
    16381 ggttaacagt tccagcatat ccgccaccat attcgggtgc atcagttgca gcgcagagag
    16441 gtttaccgac aagggcagca taatgccgcg ctcttgccag gctgcaagca atcgacagga
    16501 ctcttccagc acccagtgac cgacggtaac catcagccca cagcactcaa tgcgatcgat
    16561 taagccatcc ggcaggtccc aactgccatc cggttgctgg atacgcagta acacttccgc
    16621 actgaccagt tttccgctgg tcatctcgac ctgtggctgt aaccaaatag caaactgatg
    16681 attttccagt gcattaagga tatcgctctc ttccgtcaac cgcttctggg cggcttccat
    16741 ctgctgcgga tcaaagaact gaatctgatt cttgccttta tggcgagcgg taaatgccgc
    16801 agaaatagcg cgactgtaaa gctgttcggc ggtgagatcg ccgtagaaca tcgccacgcc
    16861 aatgctacag tgcggacgga gttgaatacg ttcaatcggc aggcgctcgc tcatgatagt
    16921 gagcacttgc tgacctaagg tgattgcgtg ccacggttcc tgtacaccgt tggcaatgac
    16981 agcaaagtca taaccgctaa tctgcgcgag gatcatacgt ggcgacagta ccgatttgag
    17041 tttttccacc agcgtcagca gcagaatttc tcgttgcgcc tctttcagca cgcccgcagt
    17101 atcacgcagg gtttcacagg tgatgatcat cagcgcggtg gtttgtttac gcgcgacaac
    17161 ttgctccagc atctccatca gcaaggcttt gttcggcaaa tccgacaccg ggaagcgcat
    17221 cgcattttcg ttctgttctt cataatggcg ctgcagcaat tgctggttga ggttgtaact
    17281 gcgcaccaac ataccgattt catcgtcctg atgcagacgc ggtaatgcca gttggtgacc
    17341 aacaagctcc tgggctggga tggcgttaag ttcgcgagca atattgcgta acggatgcaa
    17401 aatcaggcgg ttaatgcacc agctgatggc gacggtcagg ataagcgaca aaagtaagta
    17461 aatggtcact aacgttgaga gggtgctcat cacgaactta tacatacgga aggaatccgc
    17521 ctgtagtacc agataggcaa ttggctgcgg gtttgccgga cgttcgagcg agtaaacgcc
    17581 cagcgagatt tgaaccggta gctcaaacag gcgagtaacc attaccggca ccgggcgctc
    17641 tggaataaaa cttttacgca gcgcctggaa ctggttaggc agcactacat cggcacggct
    17701 gaccacgcct gccggtttaa tgctggcaag aatagcttcc gcttcgggaa tatcgccttt
    17761 caaaatagct gaagataagg gttcgcggac agagcgagca atgctttcca gttgcgtagc
    17821 cgtgttatag cgattctgct ggaccagatg gaacagcaaa acggtgcaaa aaataaaaac
    17881 gaacaccagg acaacggctg ccaccattgc catctgcttg attgttaacg agcgacttac
    17941 gcgcaaattg tctccccccg gaaatcccag attgccgccc gagtataccc gatcgcagcg
    18001 gcattaagag aggcgctatc tgaaaactta ccagtcggcg taaggtatca gcggctgcgg
    18061 cggtaaatcc atgtcaccct gccatccggc ggcggaataa cgtacgtaga gcaggaaatg
    18121 gctgggtgcg taatctttcg cctgctggat atcaattgcc gtgccaacaa accagttgga
    18181 agtaacacgt cgttcaagta atgcccgcgc cgtgtagccg aagccctgac tgctgccgcc
    18241 atcgttggat tgccgcgcag cttcttcctg ccagtcggtc gggatcagat tcatcagcgg
    18301 ataacgcggc atggttttgg tgcgtgaatg cgaccacgag ccagacgccc ccagctccca
    18361 cgaccagttt tccgtgcgct cccgccacat caccggtatg gcaaacgaca ggtattcctg
    18421 cggactgtag taaccgccct gaccgagtga gtagccactc agatctttgt cgtaatgcca
    18481 gatcatgttg ttcaggccga ttgtgacgcg gcgattgttc tggttaatga ccttatagta
    18541 atagcccgtc atccagcgca cgcgccagtt atcttcgaca tttttaccgg ttaactggtc
    18601 gccactaagc gatgcccaga cgccgtttgc ttcaccttta tcgtagctca gacttagccc
    18661 cacaccgtcg gcacgtacgc caccccattt tttcccggta ttgctcgggg agtctttttg
    18721 cccaccaaag gccagcaaag aactggagat gggccgacgg tgggcgttaa cggtgtaacc
    18781 cagcggcccg atatcatcgc tgtaactgat gccgccgacc acatccacca cgttgaagcc
    18841 catcggcgtg gtaccgatat cccagctcca gacgtcattt cgccagccga ccgccacgct
    18901 ggcaccggaa tccgactggc tgcggttgcc gctacagtcc tgtaatgtac aggtgcccca
    18961 gttatcatcc catttaccat cggcattagt ggagaaactg ccgacgttca tattgacgaa
    19021 atcactgcga aagaacatcc gcccgtcaga atacggcgca tccacctgca acatggtagt
    19081 gtgcgctttc agatcggagt aaccaccggt gccgctcgaa ccccagtaat catgctcgag
    19141 ggtgacgtta agatcctgct ggcgatagag gtccgccgca tcgctgcgca cgccgcgttt
    19201 cagccagtca tctttctcgt cgttacgggt cagtcgggta aaggtgtcgt tatcctgcgg
    19261 acgcgtcgtg gtcacaccgg atgcgaccat ggcgtctttg taggtttcca gcgcctgcgt
    19321 tggatcgccc gcctgcgctt caaacttcgc accgtcgcgc agcaccatcg cgctttccat
    19381 cgacggtggc tgagattttg cctgcgggat caacttatta aacgtctgct gcgccgctgc
    19441 ggtatctcca agctgcgcct gcgccagcgc cacgcgccgc tgtgtgttca gcgaggcgtt
    19501 atcggtagcg ggcagtttcg ccagctggct acgtgccgcc gctgtgtcac cggcagcaat
    19561 atccacttcc gtcagcccca gaatggcatc ggcgttagtt ggctccagcg tcaggacatt
    19621 ctgatatgca gcgcgggcgg cggtgtaatc acgtcgctgc tgcgcccagt cagccagcgt
    19681 gaggtcaata cgcgtggaag gtggttgctg acgcagcatc gcttccgctt cagcttcttt
    19741 gccgctttct cgcaggcgat tagcggtttc cagcacctga tcgctttgca gtcgattaac
    19801 cagctcctga atattgctgt tccactgcgc gcgcggcagg ctgttgatat gcgccagcgc
    19861 cgctctgtcc tggtcatgac cagagaggta cagcccgtaa gcgtaaacct gctccgggtc
    19921 gttcggcttc tgctgcgcca gattgcgcat taacgtatcg gcctggctgc gttgtccggc
    19981 ctgccagaga tcctgcgaaa gtcggtaagt aatccatacg ctgccggggt ccagcgccag
    20041 tcgttgccgc tgaagtgctg ccgcctgcgc ccatttgccc tggttttcca gtgcctctgc
    20101 ctgctgtgcc agacggtcgt tttgcaggct gcgttcgata tcatcaatgc tacgccgctg
    20161 actggcagag agcgaggcga taaacgcttc agctttttct ggcgattgct ggcggtaaat
    20221 atttgccagc ccgcgcacgg cgttagtgtt gccgctgtcc atacgcaagg tctgctgata
    20281 ataacgttcg gcggcgggat aatcttttcg cgccatcgcc acatcgccca gccccagcac
    20341 tgcataactg tcggtgttat cgacattacg cgcctgctgg aacaggcgtt ctgcccggtc
    20401 aggattattg gctttcagcg cagcatcgcc ctgctggatc gccagccagt agcggtttac
    20461 tttcagcaga ctgttccatt tgtcgttgtt gctgctgtgc gggtccagtg cgagggcttt
    20521 ttccagattc gccactgcat tggcgcgatc gcctttctga gaatacgcct ggcccagcgc
    20581 ccccagagct tcactgtctt tcgggttcgc ccgcaccgcc tgttgtagtt cgggaatggc
    20641 tttacccgcc ataccagagt ccaccgccgc taaaccttgc gcacgagcgc ggaaagcagg
    20701 atcggccagc tgtttttgct gttctgccag ttgcgattgc gcagccgcca cgctatcgcc
    20761 atcactaaag atcgagagat attttttcag cgccgacaca ctggcatcac tgacgggcat
    20821 gtctttaatc tgcccgtacc agattttaga ggccccttcg cgcccggcgt tcgatttcgc
    20881 catctgttcc aggacggcaa aaccttcgtc acggcgatcg ctactaaaca gcaatagcgc
    20941 cagattgttt tgcaggcccg tattgcccgg tgcatccgca ttgatgcgtt ttaactgatt
    21001 aatcgcttcg ccacggcgag ccggaatttt cgccaccgta ctccagtact cgacagcaat
    21061 gtcaccttcc ggcggcgcac cgttgaacag tttgttgtaa ctcgccacag cttcttccgc
    21121 atgaccggta gtcgcctgca atcttgcctg ttgcagtgcc tgacgaccat ccggcgtgga
    21181 aagtagcatc gtagtccgcg acgatttata cgcatttgaa ctcggcgcta actgcgacag
    21241 ccgatcgagc tgtttttgcg cgccatcaat atcgccctga cgtaacaaag aacggaaacg
    21301 ggcggcaacg acgtccgggt tattcggatc aataagttcc agccgatata acgactgttg
    21361 caccagatct tcacgatggg tcgcttcgcc taaccgaact tgctccagca actgttgctg
    21421 agcggttggt gctgcctcga ccatcggcat gacggccaga ccgagggaaa gcgtgaatat
    21481 gtttagtgtg aatttgcgca ttcctggccc cagtcaggta ataactcacc ttttgtcgag
    21541 aagcggaaac ggtgttgatc ccagccttgt ccaaacaggg tcagcacata gttgtaatag
    21601 gcatcgctgc cgggaaagtt atcggccacg cgctggcgct gaacggcctg cgcatcgcgg
    21661 ttttgtaaaa aaggcagcat ggcggcagaa aaaccgaccg gtcctttacc ctgcgctttc
    21721 cccgtagcca catccacttt ttccggcgga taaccgtttt tctcggtgaa tgtcgccatc
    21781 ggtttaaacc ggttgagcat ccgcgctttt tgcggatcgc tgtcaggcat catgcctacc
    21841 cacatgtaaa cgcggatagc gtcgtagctg ctgatcaatg ttttttcggc ttttagctgc
    21901 cagcctttgt ctttctcata gcgcacccag tctggcgaaa agcctttcgg ggcggtttcc
    21961 agcaataaac gttgattggt ttcgcgcagc gtggtccacg gcgcgccaaa gcgggtgaaa
    22021 tactgcgcca gcgtcggcgg caggtaactg gggttaaaac gccagctgtt atcctcagca
    22081 aatcccactt tgcccggcaa caacatggaa cccagcccag gcaccgtcac cacttcctcc
    22141 cgcgcaatac gttttaacaa cgcgctgccg atgtcggtat aacgctgctc tttccacaaa
    22201 cgccccgcct ccagcaacga ccaggccatc cagacatcac catcggaggc cgaattgctg
    22261 tccagcactt cccacttact gttctctttc ttgccccaca gccaggcggg caaacgttct
    22321 tttaaagaac cctgagcgag attgttctgc gtccagtcga gaatattatc gaaagctgca
    22381 cggtcgttag ccgccagggc aaagaacatg ccgtaacttt gcccttcgga ggtggtgatt
    22441 ttgcgcgcgt cgctggggtc gatgacgcgc ccttcctgac tgatgtaatc ctttttaaac
    22501 tgctcccagg caggccaggt acaggctgcc tgaacactaa aggcagccag cagcagcatc
    22561 gtcacgattc cactacgcaa cacattcatc ttcaattact cgttatccgg gttaagacga
    22621 cgacgactaa taattcgcag cagacgccac agtacccatg ccagcaatat cacactgata
    22681 gccgccagca gaatcggatg gtttgccagc gcataccaca cgcgctcgaa ccacggcaga
    22741 tgacctacgt aataaacgtc gccaacacgt aggctgttga taccggactc gcggatcacc
    22801 gcgaccgaac cgaacatggt ggcgcgtttg ccgctatcgt tcaccgcatc gttaagcatt
    22861 tcataaccgc gtgggctatc tgccaacagc gcaatcacgc tgcgctggtc gttatacggc
    22921 gactggaagc caatcaccgc cgccatcgca ccggaagagg tcagcgttga ccgggtttct
    22981 gccgcgcgat cgctctcgtc cggcacaatg ccggggaacg gggtctggcg catcggtgtt
    23041 ttcacccagc tttcggtcgc ctgcaccaat aggtcgatct gcttatcgtc tttcagtttg
    23101 tccgggatac caccgatgat catgatgtcg gcatctttgc cctgaatggt gctgccatca
    23161 tcggtcaccg tcagattaat cgccgggaag cccgtctgtg cgccgataaa accaacagta
    23221 ttcagcaacg tttccatctg tgcttcgtta ggcgctttcg gcatcacggt gatggtttgc
    23281 gacagatccg ccatccggct gaatgggaag cccgcgttag caaaggcgcg tagatccggc
    23341 atcgggatga agtggtaata cttcgagaag tcgatggtgg agtcgtcacc aatcaccaca
    23401 tgattctgca ccggctggaa ggtaatacag ttatccaccg aaccgcccgg catcgggttc
    23461 atatactcaa agtcgaagcg cagctggttg gtcgcgccca gtttcagcgc cggaatagag
    23521 acatctgttt tgccatccag caaaccttgt aataccggaa tccgcagcag caggcggttc
    23581 gcctcctgtt tgctgctcag gttgaaggat tgcaggaact ggttattcag gctgatatcc
    23641 atccgcgaac tgtctttcac cggcggcatg gtgtagcggt aattaatatc catatcaatg
    23701 ccggtactgc gcatcaggta gagatccggc ggcaggttta gcgaaacgtt aatcgctgct
    23761 ggctcaagac cgctggattg taactgttct tcataggttt tcagttcgcc aaatgtgacc
    23821 ggacgatcgg tacgtaccca gttcggcgca tcgtacggct tacgcggtag cagcggtttc
    23881 acttcattca ctaccacgct ttcaccacgg aacagaatgt taccctgagc gatacctttc
    23941 gctgcctgca acaggtcttt gtcgtcacga ccaaacacca ccagcagttt gacgtaagga
    24001 ttctgcggat ggttaatcat ctcaatcaca ggggctttta ccgccggatg atcgcgcagg
    24061 aagtccggcc gtttgtcgtt ggttgcaaag acaatggcat tacgatccgg cagttggtta
    24121 tagagcaccg ggaagttctg cccacgccag ccagaacgcg aaccaaacca cgaggcgaca
    24181 atggcagagg cttgttgcag cccaacatcc ggcgcacccg caaagaccat cggcaaggtg
    24241 ttggtgcggt tatcacgcgg atcaaagaat ggcaccggga agtgtgacag gtcattcttc
    24301 acattcaggg tctgataggt cagatccagt ccactgctcc gcccaacatc cagccaaagc
    24361 gtggtgctgg ccgggttttc gcacacgtcc tgataatggc cgacaaactc cagccgcaca
    24421 cggttgaagt cggtaataaa cagtgggtta atgggcattt gcgccagcgt ttttttaccc
    24481 aactgttctt tggtcactgg cagcacgccc atcagctcat cattgagata aacctttaac
    24541 tgcgactgga caggcagtaa cgatggcgat ggcgtgtatt cgaggttgag catcgccttc
    24601 gtcaccactt catcgctgcg cataccaaac tcaatgctgc cgttcgggtt aatgccacgt
    24661 agcaccatgc tgcccggcgg cggcgcaatt tgcgcaaagg tcagcttcac atcacgcgaa
    24721 ggaccgttct gcgccacgac tggcgcatca gcgccctgca cgcccggcat cacttgcccc
    24781 acctgcggat tttgttccgt ctgggcggct acagctggct cagcattgat cagtggttgc
    24841 gttgctggcg tcgcctgcgt catgaaagag gggaacgcac tcatccccat agccactgca
    24901 caaatccaga atagttttct tttcatcgtg ttatcatcat tgttgagcca aagcctgatc
    24961 cgatggttgt gccgtttcgc tccgctccgg gcggcgcgga ataaacgata caacccagga
    25021 aaccagagaa gtcagcacac ggaatatgcc cttcaccgaa gaaggcgcaa actccgccag
    25081 atggcggtag ccacggaagc cgagcttcag aatatccagc agactttcca gcggcttatc
    25141 ttccgggtag ctgtcctgcc agagcgccca tgtatccgca cgggcaaacg tacactgcac
    25201 aaaatcgata tgttgctggg tggtgagcgg cattaatttc agcccaactt cattacccat
    25261 cacgcgcgcc acctgggtcg ggaagacgta ttcctgctga ccgcgtttaa gcaacagatt
    25321 cactttctgc ccttccagaa tctgcgcctg accgttgatc ttgatcccca aaccaccgtc
    25381 ggagaaatcc tgaacggtac acgagaagag gtgaccatct tcgcgggcaa ttgccgcggg
    25441 catcgtcatc tccacgcggt gcgatcggcg tacctgtttg ctttctaccg ataccgcaac
    25501 tgcgccgcca agaacaatca ggttgtagaa cacccacacc atactgacga ccacggtgag
    25561 catctcggtt ggcgggccat agaagtagcg ccagatgcct accgcaacgc ccatcaggtt
    25621 gagtagaaca aggaagatgt agggccgcga gatcacccag tcgacgtact cttcttccac
    25681 cagtccacct ttggcggtga cgttaaattt gcctttgtgc gggttaatca gcgccaccag
    25741 cgtcggtggt gcgatatacc acgccagcac cgtttcgtag atttcactcc agaaagagtg
    25801 gcgatatttg ccctggatct tggagttggt caggctggca tggatcatat gcggcagcac
    25861 gaatagggcg atcatcaacg ctggcgcata gatgatgtag gcatgaagca gcaggaacgc
    25921 cagcggcgca gtcaggaaga tcagtcgtgg aatgcccgac aagaagtgga acatggcgtt
    25981 gacgtagcac agccgctggg cgaatttcag ccctttaccg gtaagcgggt tatcgagacg
    26041 gaagatttgc accatcccgc gtgcccagcg aatacgctga ccgatatgcg ccgacagact
    26101 ttcggtcgcc agccccgccg cctgcggaat acgcatatac gcggaggtat agccacgacg
    26161 gtgcaaacgc agagaagtat gcgcatcttc agtcacggtt tcgacagcaa tgccgccaat
    26221 ttcatccagc ggcttacgac gaatcaccgc acaggaaccg cagaagaaag tggcgtccca
    26281 catatcgttg ccatcctgca ccagaccata gaacagcgtg ccttcgttcg gtgttttacg
    26341 aaaacgcccc aggttgcgtt caaacgggtc cggcgagaag aagtggtgtg gagtctgcat
    26401 catcgccagc tgtttctctt tcaggaacca gcccatggtc atttgcagga acgatcgcgt
    26461 tggtacgtgg tcgcagtcga aaatcgacac gaactcgcct ttggcatatt tcagcgcatt
    26521 gttgatgttg cccgctttcg catgttcatg agtggtacgg gcgatatatt tcacccccac
    26581 gttttgcgca aactggcgaa actcttccct accgccgtca tcgaggatcc agatgttcag
    26641 cttgtctttc ggccagtcga tacccagcga ggcgtaaata gtatttttca ccacgttgag
    26701 atcttcgttg taagtcggga caaagatatc caccgacggc cacagcgaca tatctttcgg
    26761 caatggcacc ggctgacgat tcagcggcca tactacctgg aagtagccga gcaccagcac
    26821 aatccacgcg tacgtttcag cgaagagcag aataagcccg cacaccaggc tgaccggatc
    26881 gtcccagttc agcgtagagg tgtaacgcca ccagatataa cggcaagaaa cggtcagcga
    26941 cagcacaatc aacattagcg ccgagaagcg ccccggcatc cgccgtacga tcagcgctac
    27001 accccacagc agcatcagga agataaactg cgccagcggg ttaaacggtt gagtaacgca
    27061 gattaacgcc agaatcagcg agaaggtgac gatgataccg aggatcaacc gccgcgcccc
    27121 ggcactcaaa tgaccgagct cttttttctc atcaagatgc tgtgttttat ggctaacgcg
    27181 ctcaggcagc tcgttcatcc attgatggta acgtccacga atattctgca gacctgaaaa
    27241 tgcccgcctg cgcggtttcg gcgtttcttt gcgcgatgta ccgatcagta accagcatgt
    27301 ttgaatgaga taacggaccg ggtccagcgg acgcggacgc gaggcgttga tatgcggata
    27361 caggttttta tgttctgcgc gaatacgctg ccagcgcggg tgctccagcg gaataaaaat
    27421 ccaggccagg atcatccaga aacagccgag cgtcgcgctg aaagccgacg caccgtgacg
    27481 acgataatcg cgataacgcc cgataagccg cgcgttgacc ggcgggataa gcaaccaccg
    27541 ggtcaggata ctcatgatgc actcccgact ggcgttttca gcccggagta gttcaacagg
    27601 caccagttcg ccagcgtcag tatctcttca gccgccagcg catcactgcg atattcacct
    27661 actggttgct tagccgccag gcattcagcc atcgcttcat cacgatgaat gagcatcggc
    27721 agtaatcggc gctggctttg caaccaaagc tggtaaatat cgtcctgaac ctgactgcca
    27781 atacggaagt cattaatcaa aatatgtgcg ccatccggca gcgcttgctg atgcagtcgg
    27841 atatggcagt tggcatccac attgacgatt gccagcgagt gatcgcacaa actcagcagc
    27901 tggtgggtta tctgcgaggc atcacgcggt aagtcgatta aaatccactg gtaacgcccg
    27961 ctggctttta gttgctgtaa gccggagcaa atatcgctca gccgggtttg ccagtgctgt
    28021 ggattttctt gttcttcaat ggatcactga ccaaaaggca gcaaatcgag ctgcgaggtg
    28081 tagcgcaacc cagcgtcacg ccagtcctgg ccatccagca tcgctctggc ccagccctga
    28141 cggtgggtaa aatcaacatt aaatgacagg cgcaacaagt tgtccgggca ggcatcgacc
    28201 accaggacat tttctcccaa catttgtaat gaccaggcta atgcggcggt gatggttgtt
    28261 gtccccacgc ctccccgcac cccctgcaat cccagtacgg ccattcacca atcccctact
    28321 tttgttgcgc aaactctgcc agcaacggcc agcgttttaa tgccgcggcc aactgttcgc
    28381 gttgggaaat atcggcataa tcaatatcag gcagtgaaaa tgcctgcttt aacgccacaa
    28441 tatcattctg gaagatatag cctatcgcgg gatcaggcag agtatctggt tcattgttat
    28501 tcattttgct tgatccctat gaaataaccg taccgttcat gagacaattc taaccgtgag
    28561 catttcgccg gtttattgtt tacggcgaga cgggaatttg ctcacttttt gactttccgt
    28621 tacgcaacag aaaaagttaa aaacgaaaag actaaaatca cacgtcttgt gaccttctaa
    28681 attgattcac atgctaaatc tgataagttt taatttcaat ggtaggttta tttcttagct
    28741 ttcgctagta aactgataat cagacaaaat ggtgacatga gggacattgt ggaccctgta
    28801 ttctctatcg gtatctcatc attatgggat gagctgcgac atatgccagc aggcggcgtc
    28861 tggtggttta acgtcgatcg ccatgaagat gctatcagtc tggcgaatca aacaattgca
    28921 tcccaggctg caaccgcaca cgtcgcggtc attagcatgg acagcgatcc ggcgaaaatc
    28981 tttcaattag atgattctca agggccggaa aaaataaaat tattttcaat gctaaatcat
    29041 gaaaaaggtc tatactattt gacccgtgat ttgcagtgtt ctattgatcc ccataattac
    29101 ctttttattc ttgtttgcgc aaataacgca tggcaaaaca ttcctgccga gcggcttcgc
    29161 tcatggttgg ataaaatgaa taaatggagc aggttaaacc attgttcgct tttggtaatt
    29221 aatcccggaa ataataacga taaacaattt tcattgttgc ttgaggaata ccgttcactt
    29281 tttggtcttg ccagtttgcg ttttcagggt gaccaacatt tgctggatat tgccttctgg
    29341 tgcaacgaaa aaggggtcag cgcccgtcag cagcttagcg ttcagcaaca aaatggtatc
    29401 tggacattag ttcaaagcga agaggcggag atccaaccac gcagcgacga aaaacgcatt
    29461 ctgagtaatg ttgctgtact ggaaggtgcg ccgccgctat cggaacactg gcaactgttc
    29521 aacaataacg aagtcctgtt caatgaagcc cgtaccgctc aggcggcgac ggtggtcttt
    29581 tctttacagc aaaatgcgca aatcgagcca ctggcccgca gcattcatac cctgcgtcgc
    29641 cagcgcggta gtgcgatgaa aatcctcgtg cgggaaaata ccgctagcct gcgcgccacc
    29701 gatgaacgtt tgttattggc ctgcggtgca aatatggtta ttccgtggaa tgcgccactc
    29761 tcccgttgtc tgacgatgat cgaaagcgtg caagggcaga agtttagtcg ctatgtgccg
    29821 gaagatatca ctaccttgct gtcaatgacc cagccgctca aactgcgtgg tttccagaag
    29881 tgggatgtgt tctgtaatgc cgtcaacaac atgatgaata accctctatt acctgcccac
    29941 ggtaaaggcg ttctggttgc cctacgtccg gtaccgggta tccgcgttga acaagccctg
    30001 acgctgtgtc gccctaaccg taccggcgat atcatgacca ttggcggtaa tcggctggtg
    30061 ctgtttctct cattctgtcg gattaacgat ctggataccg cgttgaatca tattttccca
    30121 ttgcctactg gcgacatttt ctcaaaccgt atggtctggt ttgaagatga tcaaatcagt
    30181 gccgagctgg tgcagatgcg cttgcttgcc ccagaacaat ggggcatgcc gctgccttta
    30241 acgcaaagtt ctaaaccggt catcaatgcc gagcacgatg gtcgccactg gcgacgaata
    30301 ccagaaccca tgcgacggtt agatgatgct gtggagcgct catcatgatg accatcagcg
    30361 atatcattga aattattgtc gtttgcgcac tgatattttt cccgctgggc tatctggcgc
    30421 ggcactcttt gcgacgcatt cgcgacacct tacgtttgtt ctttgctaaa cctcgttatg
    30481 ttaaaccggc cgggacgtta cgccgcacgg aaaaagccag ggcaaccaaa aaatgactca
    30541 atttacgcaa aataccgcca tgccttcttc cctctggcaa tactggcgcg gcctttccgg
    30601 ctggaacttc tattttctgg ttaagttcgg cctgttgtgg gcgggatatc ttaacttcca
    30661 tccgctcctc aatttggtgt ttgccgcgtt tctgctgatg cccattccgc gctacagcct
    30721 gcatcgcttg cgccactgga ttgccctgcc gatcggcttt gctttgttct ggcatgacac
    30781 ctggttgcct ggcccggaaa gcataatgag ccagggttcg caggtggcgg ggttcagtac
    30841 cgattattta atcgaccttg tcacacgctt tattaactgg cagatgattg gggccatttt
    30901 tgttttatta gtggcctggt tattcctgtc acaatggatt cgcattaccg tttttgtggt
    30961 tgccatactg ctatggctga acgtacttac cctggcggga ccaagtttct ccttgtggcc
    31021 agccggacaa ccgacgacca ctgtaacaac gacgggtggt aacgcagcgg caaccgttgc
    31081 ggcgacgggt ggcgcaccgg tagtgggtga tatgcccgca caaactgcac cgccaacaac
    31141 ggcgaacctt aacgcctggc tgaataattt ctataacgcg gaggcaaaac gtaaatcgac
    31201 cttcccgtct tcgctgcccg ctgatgctca gccatttgaa ctactggtga ttaacatctg
    31261 ttcgctttcc tggtcggata tagaagccgc cgggttgatg tcgcatccac tgtggtcgca
    31321 tttcgatatt gagttcaaga actttaactc cgccacctcc tacagtggcc cggcggcgat
    31381 ccgtttactg cgcgccagct gcgggcagac ttcgcacact aatctgtatc aaccggcaaa
    31441 taacgactgc tatctgtttg ataacctttc gaaactgggc tttacccagc acctgatgat
    31501 ggggcataac ggccagttcg gcggtttttt gaaagaagtt cgcgaaaatg gcggcatgca
    31561 gactgaattg atggatcaaa caaatctgcc ggttattttg ctgggctttg atggttcgcc
    31621 ggtttatgac gataccgccg tgcttaaccg ctggctggac gttaccgaaa aagataaaaa
    31681 tagccgtagt gccacgttct acaacacgct tccactgcat gacggcaacc attatccggg
    31741 ggtcagcaaa acagcggatt acaaagcgcg ggcgcagaaa ttctttgatg aactggacgc
    31801 cttctttact gaactggaga aatcgggtcg taaagtgatg gtggtcgtgg tgccggaaca
    31861 cggcggcgcg ctgaagggcg acagaatgca ggtatctggc ctacgtgata tccctagccc
    31921 gtctatcaca gacgtccccg ttggggtgaa attcttcggc atgaaggcac cacatcaggg
    31981 ggcaccgatt gtcattgacc aaccgagcag cttcctggct atctccaatc tggtggttcg
    32041 cgttcttgat ggcaagattt tcaccgaaga caatgttgac tggaaaaaac tcaccagtgg
    32101 gttgccacaa acagcaccgg tctccgagaa ctcaaatgca gtagttattc aataccagga
    32161 taaaccgtac gttcgcctga acggcggcaa ctgggtgcct tacccgcagt aagcacgaaa
    32221 aaggccgcag agtttaacct ctgcggcctg atccgggcgc aaattgccat tacggcagcc
    32281 tgacgcccgc atggcacgtt acttccgctt gttcagccag ttcacgatca tactggcaag
    32341 aataccagca atgaccggag ccgctaaatc atgccagaag gccatgccca gttctgcgag
    32401 cgtcatatag ccgcctgtat tgtaatgaca acgtttcgcg gctattcttg tattgtttgg
    32461 atgcaagaat ggcccccgtg gtgttgtcag gtgcatacct gcaacgtgcg ggggttttct
    32521 ctctccagca accaatgcca ccagggatta agcccccgca acattgcgcc tcaccggaat
    32581 ccttccggct tgacgctgat tctacgactg tttttctgtt ccggaatccg tctcatcttc
    32641 actctgtgct gcgcattccg cagaatatta tccgcttgca accgttgcat ttttcttacg
    32701 aaaaaggccg cagagtttca ctcctgcggc ctggtccggg cgcaaattgc cattacggca
    32761 gcctgacgcc cacatgacac gctacttccg cttgttcagc cagttcacga tcatactggc
    32821 aagaatgcca gcaatgaccg gagccgctaa atcatgccag aaggccatgc ccagctctgc
    32881 gagcgtcata tagccgcctg tgttgtaatg acaacgtttc gcggctattc ttgagtggtc
    32941 taggttcaag attagccccc gttatgttgt caggtatata cctgcaacgt gcgggggttt
    33001 tctctctcca gcaaccaatg ccaccaggga taaagccccc gcaacattgc gcctcaccgg
    33061 aacccttccg gcttgccgct gattttacga ttgcttttta ctgtcagcac ccggcaatct
    33121 tcctcattgc gcgatgcctt ccagcttaat ttctttattt gcaatagttg cagttcatat
    33181 ccgggctgac tccagactgg tagtgaaatg cgcaattttg tagaattgcg cctcctcgcg
    33241 acctccccag tgaatctgtt gcctcatttt ttacatactg cgtattcgac ttctccacct
    33301 gttgcgcaag agaaactggg tttattcatt tttgcgaggc cgacttcttt ctggacagga
    33361 ctttatgcag cacaacacac tatcgaaaca caatcagaaa ttgccgttta cacgctacga
    33421 cttcggctgg gttttattat gcataggcat ggcgattggt gccggaaccg tgctgatgcc
    33481 agtacaaatt ggcttgaagg gaatttgggt atttattacc gcagcgatca ttgcttatcc
    33541 tgccacctgg gtagtgcagg acatttattt aaaaaccctt tctgaaagcg attcctgtaa
    33601 tgactacacc gatattatca gtcattacct ggggaagaac tggggaattt tcctcggggt
    33661 tatctacttt ttgatgatta tccacgggat ttttatctac tctctctccg tggttttcga
    33721 cagcgcctcg tacctgaaaa ccttcggttt aaccgatgcc gatctttcac aatctctact
    33781 ttataaagtc gctattttcg ccgtactggt ggcgattgcg tctggtggtg aacgattact
    33841 gtttaagatt tccgggccaa tggtggtggt caaagtaggg attattgtcg tgttcggttt
    33901 tgcgatgatc ccgcactgga atttcgccaa tataaccgcc ttcccgcaag cctccgtctt
    33961 tttccgcgat gtcctgctta ccattccatt ttgcttcttt tctgcggtat ttattcaggt
    34021 acttaaccca atgaatattg cctatcgtaa acgggaagca gataaagtac tggcaacccg
    34081 gctcgcgctg cgtacccatc gaattagtta tatcacgctc atcgcggtga tcctgttttt
    34141 tgccttttcg tttaccttct caattagcca cgaagaagcc gtttctgcct ttgaacaaaa
    34201 tatctcagca ctggcgctgg ccgcgcaggt gatccctggg catatcattc atatcacctc
    34261 tacggtgctt aatatctttg ccgtactgac cgcattcttt ggcatttatc tcggtttcca
    34321 cgaggccatt aaaggcatta ttctcaatct gttaagccga attattgata ccaagaaaat
    34381 taactcacgc gtgctgactc tggcgatctg cgcttttatc gtcattacgt tgacgatttg
    34441 ggtttcgttt cgtgtatcgg tgctggtgtt ctttcagttg ggaagcccgt tatatggcat
    34501 tgtgtcgtgc ctcattccgt ttttcctgat ctataaagtc gcacaactgg aaaaacttcg
    34561 cggatttaaa gcctggctaa ttctgcttta cggcattttg ctatgcttgt cgccactgtt
    34621 gaagctgatt gagtaaaccg gagcgcatgg ccccggtttt gtgagttaac gctgcggatt
    34681 ttcatcctga tcaacagcaa aacaagctac cagttgaccg ccgtagtctt ttagctgcgg
    34741 ctgcaactgg gtgcaggggc cgaagcgccg acgacagcgg gcgttgaagg cgcaacccgg
    34801 cggaggattc agcgggcttg gcagttcacc ggtgagtttg atgcgttcac gacgatcgtc
    34861 cgggttcagg cgtggcgtcg cggagagcag cgcctgggtg tacggatggc gcgggttatt
    34921 gaagatttgg tccttcgttc ctttctccac gcagcggccc aggtacatca ccatcacttc
    34981 atcagcaatg tgctccacaa ccgacaggtc gtgggagata aagacataag aaagccccaa
    35041 ctcctgctgc aaatccatca tcagattcag cacctgcgca cgcactgaga cgtccagcgc
    35101 agaaaccggt tcatcggcaa tcaccacgtc cgggtcgagc atcagaccac gggcaatagc
    35161 gatacgctga cgttgtccgc cggagaacat atgcggatag cggtcatagt gctcggtttt
    35221 caggccgact ttcgccatca tcgacagggc tttttcccga cgctgttctt tgctgaagct
    35281 ggtgttaata agcagcggct cttcgagaat ttgcccaact tttttacgcg gattcagcga
    35341 accgtaaggg ttctggaaga caatctggat tttctgccga cgcagcttct gcgcctgcgg
    35401 atcgtgcttg agcagatcct gcccctggta atacagctca ccaccggtgg gcgtttcgat
    35461 catcgtcagc aaccgaccga gggtcgattt accgcagcca gattcgccca ctactgccag
    35521 cgttttgcca cgttcaaggt taaacgaaac gccgtccagc gccttcacca gacgttccgg
    35581 cgcgaacatg cctttcttca ccggataatg ttttttcagg tcgatagcct gcaacagcgg
    35641 ttgttgcgag gtggcctctt gcgtactcat agtgtcggcc tcccggcatc atcaagtggg
    35701 taatggcatt tggactgacg cccatcagcg agcatattca gcgccggttc ttcagcgcga
    35761 catctgtcag tggcataggg gcagcgcggg ttaagcaggc agccgttcgg gcggtcgtac
    35821 ttaccgggaa cgacacctgg caacgacgcc agacgttctt tgtcctgagc aaattctggc
    35881 agcgcacgca gcaatgcctg agtatacggg tgacgcggcg catggaagat ggcgtgcgca
    35941 tcaccggttt ccaccacctg gcctgcatac atcacgatga ttttatgtgc cgcttccgcc
    36001 accagcgcca ggtcatgggt aattaacacc agcgccatgt tctctttctg ctgtagctcc
    36061 agcagtagtt cgatgatttg cgcctgaatg gtcacgtcca gcgcggtggt cggttcatcg
    36121 gcaatcagca gttttggccg acaggcaatc gccatggcga tcatcacgcg ctggctcatg
    36181 ccgccggaaa gctgatgcgg gtaaacatcc agacgcgatg ccggatcggg aataccgacc
    36241 tgattcagca ggtcaatcgc tcgctgacgg cgggtacttt tgttgccgcc ctgatgcacc
    36301 ttaatcgctt ccataatctg gaaacccacg gtgtagcacg ggttaaggct ggtcatcggg
    36361 tcctggaaga tcatcgccac ttcggcaccc accaggttgc ggcgctcttt ttctgagata
    36421 cgctgcaaat cctggccgtt aaactccagt ttttctgcca ttacgcggcc cggataatca
    36481 atcagcccca taatcgccag tgaactgacc gacttaccgg agccggactc acccacaatc
    36541 ccgaccactt cgccctgttt tacgctgtag ctgatgcggt ctacggcgcg gaacggcgcg
    36601 ctttcgtcgc cgaaatgcac cgataattta tctacattta ataacgccat ctcgaacctc
    36661 ttactgcttc agtttgggat cgagcgcgtc acgcagaccg tcacccatca ggttaaatgc
    36721 cagcaccgtc agcaggatcg ccagacccgg gaaggtcacg acccaccagg cgctttgcgc
    36781 gaactgcaac acgtcggaga gcatggtgcc ccactcaggc gttggcggct gtgcccccat
    36841 gccgaggaaa ccaagagcag ccatatcgag aatggcgtta gagaaaccga gcgacgcctg
    36901 aacaatcagc ggcgcaaggc agttcgggaa gatgttaata aacatctgac gcatcgcccc
    36961 ggcacccgcc acgcgagacg cggtgacgta atcgcggtta acttccacca gcacggcggc
    37021 gcgggttaag cgcacatagt gcggcaaggc aacgaaggtc agtgccagcg cggcgttacc
    37081 aatcgacggg ccgaaaattg ccaccagcac cagcgccagc agcagacttg gcagcgccag
    37141 catgatatcg accacgcgca taatgatgtt atcgaccagg ccgccaaagt aaccggcgat
    37201 caggccgaga ataacgccca taatcagcga taacacaact accagacagc caaccagcag
    37261 cgacaggcgc gcaccgtaca tcaggcgcga cagcacatca cggcctacgt catcggtgcc
    37321 cagcaagtgc gccatgctgc cgccttcctg ccaggctggc ggggcgagca gtgcatcgcg
    37381 gaactgttcc gccgggttat agggtgcaat ccagttggca aagatcgcga tgaacagcac
    37441 gatgacgacg taaaccagcc cgacgaccgc gcctttgttg cgtttaaaat agtgccagaa
    37501 ctcctgtaac ggggtcatcg gcaccggtgc gctaatcact ttattttcag taacctgtga
    37561 catgatgtcc ccttacttct tatgacgaat acgcgggttc accacgccgt acagcagatc
    37621 gaccagcaag ttgacgagga taatcatcgt cgccaccagc aatacgccgc cctgcactac
    37681 cggatagtcg cggcgttgca gtgcgtcaat caaccagcgt cccagaccgg gccacgagaa
    37741 gatggtttcg gtcagaatcg cccccgccag caatgttccc acctgcaggc cgataacggt
    37801 caccaccggc agcatcgcgt tacgcagcgc atggacgata atcacccgca tgcgggttag
    37861 ccctttggcg cgcgcggtgc ggatgtaatc ctcgcccagc acttccagca tcgaggagcg
    37921 tgtcatacgc acaatgaccg ccagcggaat agtacccagc acaatggcag gcaagatcat
    37981 atgggcgacg gcatcgataa agttgccgtc ttcaccccag atggcggtgt cgattagcat
    38041 aaaaccggtt aacggattgg agtcatcgag gaacaccata tcgctcacgc gaccggagac
    38101 gggcgtcagg ttccagtgca ccgaaaccag catgatcagc atcatgcccc accagaagat
    38161 cggcatcgag tagccggtca gcgccaggcc aaccgctgtg tgatcgaaaa tggaaccgcg
    38221 tttaaccgca gccagcacac caaccggaat accgactgcc gtagcaaaaa tcatcgcgca
    38281 gacgccaagt tccagcgtgg cctgaaagcg cggcacgaac tcttcccata ctggaattcg
    38341 gcttttcatt gaaatgccta gatcgccgtg cattacgccc caaatgtaat ggagatactg
    38401 ctgccacatc ggtttatcta agccgagttc agccagcagc tgcgcgtgac gctcagggga
    38461 gatcccacgt tcgcctgcca tgatcatcac cggatcgccc gggatcatgt ggacaaaggc
    38521 aaatgtgaga agggtaatac cgataaacgt ggggatgacg agtcccaaac gtcggagaat
    38581 aaactgcaac ataacccgga ttctctatag tgacccgcag cctggaactg cgtgtctgta
    38641 ttgctcacaa atcaattccc gcccttatcg gacagggaat gaagtattgc tgccggatgg
    38701 cgctgcacgc agaggcacag cgccaccggg tatacagatt gttgacaaag tgcgctttgt
    38761 ttatgccgga tgcggcgtta acgccttatc cggcctacaa aatcgtgcaa attcaataca
    38821 ttgcagaatt ttcgtaggcc tgataagcgg agcacatcag gcagttttgc ctttgccatc
    38881 agtcttgtat ggcttttaat tattcgatag agacattttc gaagtgatgt ttgcctaatg
    38941 gatcaaccac atagcctttg acttctttgc gtaccggttc aaacacggtg gagtgagcga
    39001 tgatcagtgc cggagcctga tcgtgcatca ccacctgcgc ttgcttgtac agttcaacgc
    39061 gtttgttatg gtcgtcggta gcacgcgccg gttgaatcag atcttcaaac ggtttgtagc
    39121 accattttga gtagttagaa ccttgttcag aggcggcgca gctgaacagg gtggcgaaga
    39181 agttatccgg gtccccgtta tcgccagtcc agcccatcat taccgtctga tgttcgccat
    39241 ctttcgcacg cttgaggtac tcaccccatt cgtaggtgac gattttggcc tgcacgccca
    39301 ctttcgccca gtctgcctga atcatctccg ccatgcggcg agcgttcggg ttatacggac
    39361 gttgtaccgg catcgcccac aggtcgatgg agaaaccttt ttccagaccc gcttctttca
    39421 gcaaggcttt cgctttttca ggatcgtagg tgtagtcctg aacgtcgtcg ttatagcccc
    39481 acatggttgg cgggatcagg tttttcgctg atacacccgc gccctgataa accgctttga
    39541 tgatcgcgtc tttgttcacc gcgtaggtca gagcctggcg aacttttacg tcatcgagtg
    39601 gttttttctg cacgttatat gaaagatagc cgacgttcag ccccggcatt tccatcaggt
    39661 tgatggattt atcctgcttc atgcgagcga tatctgccgg gttcgggtac ggcatcacct
    39721 ggcattcatt cttctgcaat ttcgcgtaac gcacggaagc atcaggggta atagagaaga
    39781 ccagcgtatc gatctgcggt ttggtgcccc agtagccatc aaacgctttg tagcggatac
    39841 gggaatcttt ttggtactgc tgtaactgga acggaccggt tccgattggg ttgaggtcca
    39901 gtttttccgg tgtaccggct ttcatcatcg catcagcata ttcttttgac agaatagagg
    39961 cgaagtccat tgccagatca gcgaggaacg gcgcttccgg gcgagtcagt acgaactgaa
    40021 ctgtgttgtc gtccaccttt ttcacttcac tgatcagctc cggcaagccc atgccttcga
    40081 agtattcgta gctgccgcca gagactttat ggtacgggtt ttgcgcgttt ttctgacggt
    40141 cgaacgagaa caccacgtca tcggcgttca gttcacgcgt cggtttgaat tctttattgt
    40201 cgtgccactt cacaccttta cgcagatgga aggtataggt tttaccgtct tcgctgactt
    40261 cccacttttc agcgaggccc gggatcactt cagtggtgcc gattttaaat tcaaccaggc
    40321 ggttataaag cggtacggaa gaggcgtcat aggtggtgcc ggaggtaaac agctgcgggt
    40381 taaacccttc cggagatcct tctgagcaat aaaccagagt tttagcctga acacttgctg
    40441 cgacggtcat agccaccagg ctgagaccaa gcttcagcat ccctgacttt ttcaaggaaa
    40501 tacgcattat tctgctccaa ttgtgatgtt tgttgtttta accctttgca gtgggttgtc
    40561 gctgcctgac cttttttgtt ttttgcccgg tcgggtcaac gttatgaggt ggggatgccg
    40621 tactaattaa catcagtgta acagtgcgga tcctccataa aatgcccctc gtgacctaca
    40681 atctgtcaac agaatgtgaa aacgtcaata cagccaaccg ggatttacac caacggtgag
    40741 aatccacaaa caaagattaa aaaaacttca aacagctatt tgcagcagag aaatttgtgc
    40801 tactccaaca tgaccagaac aatcagctta atatttagca acatttggtg attaagtttt
    40861 atgcatttaa tgaaaaaaat ctgaggaaaa ggtggatatc tgagcgatta ataccatgaa
    40921 cgctaaaacg cacagcggaa aatgccaggg tcagccataa gtaacgcaaa aaaagattta
    40981 agcaaatata aaaaaagaca atggtttctg tcacaaatcc gttaatggat agtgagatat
    41041 ggggcgcaaa tttggcaggg aaatgtgtgg tgtgtaggtc gaataaggcg gtcgcgccgc
    41101 atccgacaat aagtgcggtg ccgttgcctg atgcgacgct
//
LOCUS       DAZKIV010000023        40788 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZKIV010000023 DAZKIV010000000
VERSION     DAZKIV010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40788
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.023"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(148..2139)
                     /gene="tkt"
                     /locus_tag="ACHOT6_001277"
     CDS             complement(148..2139)
                     /gene="tkt"
                     /locus_tag="ACHOT6_001277"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFP9946525.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            2417..3175
                     /gene="loiP"
                     /locus_tag="ACHOT6_001278"
                     /gene_synonym="yggG"
     CDS             2417..3175
                     /gene="loiP"
                     /locus_tag="ACHOT6_001278"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFP9946526.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(3231..3974)
                     /locus_tag="ACHOT6_001279"
     CDS             complement(3231..3974)
                     /locus_tag="ACHOT6_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9946527.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(3961..5070)
                     /gene="dgaE"
                     /locus_tag="ACHOT6_001280"
                     /pseudo
     CDS             complement(3961..5070)
                     /gene="dgaE"
                     /locus_tag="ACHOT6_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
     gene            complement(5074..5934)
                     /locus_tag="ACHOT6_001281"
                     /pseudo
     CDS             complement(5074..5934)
                     /locus_tag="ACHOT6_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(5931..6680)
                     /locus_tag="ACHOT6_001282"
     CDS             complement(5931..6680)
                     /locus_tag="ACHOT6_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HFP9946528.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(6706..7191)
                     /locus_tag="ACHOT6_001283"
     CDS             complement(6706..7191)
                     /locus_tag="ACHOT6_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HFP9946529.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(7202..7630)
                     /locus_tag="ACHOT6_001284"
     CDS             complement(7202..7630)
                     /locus_tag="ACHOT6_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9946530.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(7749..10547)
                     /gene="dagR"
                     /locus_tag="ACHOT6_001285"
     CDS             complement(7749..10547)
                     /gene="dagR"
                     /locus_tag="ACHOT6_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HFP9946531.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHAATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(10806..11726)
                     /gene="speB"
                     /locus_tag="ACHOT6_001286"
     CDS             complement(10806..11726)
                     /gene="speB"
                     /locus_tag="ACHOT6_001286"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFP9946532.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(11862..12593)
                     /locus_tag="ACHOT6_001287"
     CDS             complement(11862..12593)
                     /locus_tag="ACHOT6_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946533.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     SVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHVRVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
     gene            complement(12739..14715)
                     /gene="speA"
                     /locus_tag="ACHOT6_001288"
     CDS             complement(12739..14715)
                     /gene="speA"
                     /locus_tag="ACHOT6_001288"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFP9946534.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(14724..14855)
                     /gene="yqgB"
                     /locus_tag="ACHOT6_001289"
     CDS             complement(14724..14855)
                     /gene="yqgB"
                     /locus_tag="ACHOT6_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFP9946535.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            complement(14949..15092)
                     /gene="yqgG"
                     /locus_tag="ACHOT6_001290"
     CDS             complement(14949..15092)
                     /gene="yqgG"
                     /locus_tag="ACHOT6_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFP9946536.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            complement(15203..15454)
                     /gene="yqgD"
                     /locus_tag="ACHOT6_001291"
     CDS             complement(15203..15454)
                     /gene="yqgD"
                     /locus_tag="ACHOT6_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFP9946537.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            15510..16664
                     /gene="metK"
                     /locus_tag="ACHOT6_001292"
     CDS             15510..16664
                     /gene="metK"
                     /locus_tag="ACHOT6_001292"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFP9946538.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            17100..18494
                     /gene="galP"
                     /locus_tag="ACHOT6_001293"
     CDS             17100..18494
                     /gene="galP"
                     /locus_tag="ACHOT6_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFP9946539.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            18571..19068
                     /gene="yggI"
                     /locus_tag="ACHOT6_001294"
     CDS             18571..19068
                     /gene="yggI"
                     /locus_tag="ACHOT6_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFP9946540.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            19163..19870
                     /gene="endA"
                     /locus_tag="ACHOT6_001295"
     CDS             19163..19870
                     /gene="endA"
                     /locus_tag="ACHOT6_001295"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFP9946541.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            19950..20681
                     /gene="rsmE"
                     /locus_tag="ACHOT6_001296"
     CDS             19950..20681
                     /gene="rsmE"
                     /locus_tag="ACHOT6_001296"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFP9946542.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            20694..21644
                     /gene="gshB"
                     /locus_tag="ACHOT6_001297"
     CDS             20694..21644
                     /gene="gshB"
                     /locus_tag="ACHOT6_001297"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFP9946543.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            21753..22316
                     /gene="yqgE"
                     /locus_tag="ACHOT6_001298"
     CDS             21753..22316
                     /gene="yqgE"
                     /locus_tag="ACHOT6_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFP9946544.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            22316..22732
                     /gene="ruvX"
                     /locus_tag="ACHOT6_001299"
     CDS             22316..22732
                     /gene="ruvX"
                     /locus_tag="ACHOT6_001299"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFP9946545.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(22908..23888)
                     /gene="yggR"
                     /locus_tag="ACHOT6_001300"
     CDS             complement(22908..23888)
                     /gene="yggR"
                     /locus_tag="ACHOT6_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFP9946546.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            23906..24610
                     /gene="yggS"
                     /locus_tag="ACHOT6_001301"
     CDS             23906..24610
                     /gene="yggS"
                     /locus_tag="ACHOT6_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFP9946547.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            24628..25194
                     /gene="yggT"
                     /locus_tag="ACHOT6_001302"
     CDS             24628..25194
                     /gene="yggT"
                     /locus_tag="ACHOT6_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFP9946548.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            25191..25481
                     /gene="yggU"
                     /locus_tag="ACHOT6_001303"
     CDS             25191..25481
                     /gene="yggU"
                     /locus_tag="ACHOT6_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFP9946549.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            25489..26082
                     /gene="rdgB"
                     /locus_tag="ACHOT6_001304"
     CDS             25489..26082
                     /gene="rdgB"
                     /locus_tag="ACHOT6_001304"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFP9946550.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREQAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            26075..27211
                     /gene="hemW"
                     /locus_tag="ACHOT6_001305"
     CDS             26075..27211
                     /gene="hemW"
                     /locus_tag="ACHOT6_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFP9946551.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(27366..28373)
                     /gene="yggM"
                     /locus_tag="ACHOT6_001306"
     CDS             complement(27366..28373)
                     /gene="yggM"
                     /locus_tag="ACHOT6_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFP9946552.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAVSDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(28490..29536)
                     /gene="ansB"
                     /locus_tag="ACHOT6_001307"
     CDS             complement(28490..29536)
                     /gene="ansB"
                     /locus_tag="ACHOT6_001307"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFP9946553.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(29712..30431)
                     /gene="yggN"
                     /locus_tag="ACHOT6_001308"
     CDS             complement(29712..30431)
                     /gene="yggN"
                     /locus_tag="ACHOT6_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFP9946554.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(30615..30941)
                     /gene="yggL"
                     /locus_tag="ACHOT6_001309"
     CDS             complement(30615..30941)
                     /gene="yggL"
                     /locus_tag="ACHOT6_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFP9946555.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(30941..31660)
                     /gene="trmB"
                     /locus_tag="ACHOT6_001310"
     CDS             complement(30941..31660)
                     /gene="trmB"
                     /locus_tag="ACHOT6_001310"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFP9946556.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            31821..32873
                     /gene="mutY"
                     /locus_tag="ACHOT6_001311"
     CDS             31821..32873
                     /gene="mutY"
                     /locus_tag="ACHOT6_001311"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFP9946557.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            32901..33176
                     /gene="yggX"
                     /locus_tag="ACHOT6_001312"
     CDS             32901..33176
                     /gene="yggX"
                     /locus_tag="ACHOT6_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFP9946558.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            33241..34320
                     /gene="mltC"
                     /locus_tag="ACHOT6_001313"
     CDS             33241..34320
                     /gene="mltC"
                     /locus_tag="ACHOT6_001313"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFP9946559.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            34522..35778
                     /gene="nupG"
                     /locus_tag="ACHOT6_001314"
     CDS             34522..35778
                     /gene="nupG"
                     /locus_tag="ACHOT6_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFP9946560.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(35828..37963)
                     /gene="speC"
                     /locus_tag="ACHOT6_001315"
     CDS             complement(35828..37963)
                     /gene="speC"
                     /locus_tag="ACHOT6_001315"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFP9946561.1"
                     /translation="MKSMNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVEMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVLYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(38125..38208)
                     /gene="yqgH"
                     /locus_tag="ACHOT6_001316"
     CDS             complement(38125..38208)
                     /gene="yqgH"
                     /locus_tag="ACHOT6_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFP9946562.1"
                     /translation="MHAISLHSLAYRRFARNVSLSMLLLMR"
     gene            38361..39068
                     /gene="yqgA"
                     /locus_tag="ACHOT6_001317"
     CDS             38361..39068
                     /gene="yqgA"
                     /locus_tag="ACHOT6_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFP9946563.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPAMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            39174..39249
                     /locus_tag="ACHOT6_001318"
     tRNA            39174..39249
                     /locus_tag="ACHOT6_001318"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39207..39209,aa:Phe,seq:gaa)
     gene            39447..40712
                     /locus_tag="ACHOT6_001319"
     CDS             39447..40712
                     /locus_tag="ACHOT6_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9946564.1"
                     /translation="MALTDAKIRAAKPSDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRQPISENTVNKALRVMGYDT
                     PQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKFISPFEYAKINNPLKQ"
BASE COUNT         9871 a        10933 c        10216 g         9768 t
ORIGIN      
        1 atatcgtgat gcatattaca taattgatat ttattgcata taaatatttg tgatctacaa
       61 cacgctttat ctattgcttg tccgcaaacg gacatatcaa ggtaataaaa aaggtcgccg
      121 aagcgacctt tttacgcgaa atgctaatta cagcagttct tttgctttcg caacaacgtt
      181 atcaacagtg aagccgaact cttcaaacag cagctctgcc ggagcagatt caccgaaggt
      241 ggtcataccg acgatagcac cgttcaggcc aacatacttg taccagtagt cagcaatacc
      301 cgcttctaca gcaacgcgtg cagtaaccgc tttcggcagt acggattcac ggtaagcagc
      361 atcctgcttg tcaaatgcgt cggtagacgg catggacacc acgcgcgctt tcacgccttc
      421 ggcagtcagt ttttcgtagg cagcaacagc cagttcaact tctgaaccgg tagcgatgaa
      481 aatcagttcc ggctgaccgg cgcagtcttt cagcacataa ccaccgcgcg cgatgtttgc
      541 cagttgctct tcagttcgtt cctgctgcgc caggttctga cgggagagga tcagtgcggt
      601 cgggccgtcc tgacgctcaa caccgtattt ccacgcgacc gcggattcaa cctggtcaca
      661 cggacgccat gtagacatgt tcggggttac gcgcagagaa gcgacctgct caaccggctg
      721 gtgagtcggg ccgtcttcgc ccagaccgat ggagtcgtgg gtgtaaacca tcacctgacg
      781 ctgtttcatc agcgcagcca tacgtacggc gttacgtgcg tattccacga acatcaggaa
      841 ggtggaggtg tacggcagga aaccaccgtg cagggagata ccgttagcaa tcgcggtcat
      901 accgaactcg cgaacaccgt agtggatgta gttacccgca gcatcttcgt tgattgcttt
      961 agaaccagac cacagggtca ggttagacgg tgccaggtca gcggagccgc cgaggaattc
     1021 cggcaacagc gggccgaacg cttcaatagc attctgagac gctttacggc tggcgatttt
     1081 cgccggatta gcctgcagtt tagcgataaa ctctttcgct ttggcgtcga agtcagacgg
     1141 catttcgcct ttcatacggc gggtaaattc agccgcttcc tgcggataag ctttcgcgta
     1201 agccgcaaac ttctcattcc atgcagattc tttcgcctgg cctgcttctt tcgcatccca
     1261 ctgcgcatag atttcagacg ggatttcgaa cggtgcgtat ttccagccca gctgttcgcg
     1321 ggtcagagcg atttctgcat cacccagcgg cgcaccgtgg gagtcgtgag taccggcttt
     1381 gttcggggaa ccgaaaccga tgatggtttt gcacatcagc agggacggtt tgtcagtcac
     1441 tgcgcgcgct tcttctactg cgcgtttgat ggatgccgcg tcatgaccgt cgatgtcgcg
     1501 aataacgtgc cagccgtaag cttcgaaacg cattgcggtg tcgtcggtga accagccttc
     1561 aacgtgacca tcaatagaga taccgttgtc atcgtagaac gcaatcagtt tacccagctt
     1621 cagcgtaccc gccagagagc aaacttcgtg ggagatgcct tccatcatgc agccgtcgcc
     1681 catgaaggcg taggtgtagt ggtcgacaat gtcgtggccc ggacggttaa actgcgccgc
     1741 cagcgttttt tctgcaatcg ccataccgac tgcgttggca ataccctgac ccagcggacc
     1801 ggtggtggtt tccacaccag cggtatagcc tacttccggg tggcccggag ttttagagtg
     1861 cagctgacgg aagtttttca gttcttccat cggcagatcg taaccggtga ggtgcagcag
     1921 gctgtagatc agcatggagc cgtggccgtt ggacagcacg aagcggtcac ggtcagccca
     1981 ggacggattc tgcgggttat gtttcaggaa atcacgccac aggacttcgg caatgtcagc
     2041 catacccata ggggcacccg ggtgaccgga tttggctttc tgtactgcgt ccatgctcag
     2101 cgcacgaata gcattggcaa gctctttacg tgaggacatt ttgactccag atcggatgat
     2161 gaagggcacg cccttaacga cttgacgaca gcgcgttttg ggctacgccg gaaaatttgc
     2221 caacaattta ccgcaagccg cgcgtcatgt acatggaaca tccttttgcc gcttcagaaa
     2281 tctctggatc atgctcgcat gttgcgcaat ctactcgccc gtccgctgcg cttttcctta
     2341 tactgagact gagcgtcggt tcacctgcaa acggcgcatt tttagaataa tcctgacctt
     2401 gtgcggaaga aaaaacatga aaattcgcgc cttattggta gcaatgagcg tggcaacggt
     2461 actgactggt tgccagaata tggactccaa cggactgctc tcatcaggag cggaagcttt
     2521 tcaggcttac agtttgagtg atgcgcaggt gaaaaccctg agcgatcagg catgtcagga
     2581 gatggacagc aaggcgacga ttgcgccagc caatagcgaa tacgctaaac gtctgacaac
     2641 tattgccaat gcgctaggca acaatatcaa cggtcagccg gtgaattaca aagtgtatat
     2701 ggcgaaggat gtgaacgcct ttgcaatggc taacggctgt atccgcgtct atagcgggct
     2761 gatggatatg atgacggata acgaagtcga agcggtgatc ggtcacgaaa tggggcacgt
     2821 ggcgttaggc catgtgaaaa aaggaatgca ggtggcactt ggtacaaatg ccgtgcgagt
     2881 agctgcggcc tctgcgggcg ggattgtcgg aagtttatct caatcacaac ttggtgatct
     2941 gggcgagaaa ttagtcaatt cgcaattctc ccagcgccag gaagcagaag ccgatgatta
     3001 ttcttacgat cttctgcgcc aacgcggcat cagcccggca ggtcttgcca ccagctttga
     3061 aaaactggca aaactggaag aaggtcgcca aagctcaatg tttgacgacc atcctgcatc
     3121 cgccgaacgc gcccagcata ttcgcgatcg tatgagcgcg gatggggtta agtaagtcag
     3181 acataccgat ctacaacata attgtaggtc ggtaacgcct gaatccccga tcagagtagt
     3241 gatttcactt tatttagcaa aacggctaca tcatccggtc gggtatatcc cgtatcaggg
     3301 tctataatcg agctgtaaac atgtggcata atgcgcggca cccccgcctc cagacaagtt
     3361 ttcagaatca cactgaagtt atccaaatca atcccgccag tcggctcaat cagcgtcata
     3421 ccattgcgcg ccgccgtggt cgccagcgcg tatagctccg gaagcgacct ctcgccgccc
     3481 atcggaaaga acttcgccgc gtgtgcgccc atatctagca tcattcgcac cgccgtatcg
     3541 caggagacgc gggcaggcgt accctggctg ctgctaacgc cggtggaaat cagcacttcg
     3601 cccggcgtcc ccgtcgggct aaccagcgcg ttgacatggg tctgctcacc gcccgtagcc
     3661 gcaagcgcgc ctgccgcgaa accacaaccg gtaaaggtct ggttaacgtg tgctggatga
     3721 atctgcgccg caatcatcgc agctttgtaa tactgcgccg ggtcacccgc ccccagacct
     3781 actgaaatgg acggtatttc cgccatccaa cgtttaactt cctggatccc ctcatccacc
     3841 gaggagaact gcgcggaaag cacgccaatc actgcgtggc cttcggcggc gtcaaagatt
     3901 tcacgggcat tggcgatatc tttcgccagc acattaattg ccacgcgatt acgatagaaa
     3961 ttaatctttt gcatgatctg caatctcctt cagtcgcgcc acgataagtg ccatttcccc
     4021 ttccgccacc gtacgcggat caaggctaaa aacgccctgg tggaggttat accgccgtgc
     4081 ataaatcgcg atatcgccac cacgcagttg cgcttcgacg acgcgagcgt caactcccaa
     4141 ttcctgcgca tttactcgaa tacgaatacg ccagattgca cgcccggctt catcctgctc
     4201 aatatcagcc gttaacccgt gaatcgcaga aatcgcttca accactggct ggagctgttc
     4261 cgcggtgatg actgcctgcc cctagtgata attctccagc gcgtaaacca gcccaaccat
     4321 gttctccttg ccgattttca tcgccctggc aatgccgtga tgctgcgctt tgcaggctgc
     4381 aatccagatt tttttaccag tgataaaccc ggaggtcggc gcgttaaagg cttttgcgcc
     4441 gctgtagacc accatatccg ctccgctcac cacccaggca cgtaaatcct cttcggcggc
     4501 ggcatcgaca atcagcggca ggttgtacat ctgcgcgaca tgtgcgaagt cctcgatgtt
     4561 caacattccc ttctgcacgc agtgatgcga cttcacatac agcagcgcgg cggtcgcgtc
     4621 ggtaatggca ctctccagct gccagagagc cgccaggtta cttgaaccca cctccacaac
     4681 gcgcccgcca cctaagcgga tcgcgctgag gatcgatgca ccgtaatcga cgttatggcc
     4741 gcgcagcatc accacttcat tggccatgcc ggagctatcc ggcatgagcg ccacccgggc
     4801 cttgtcgccg cgtgtaatgg cggcggcgac agcgatggcg atccctgctg aggcacagga
     4861 ggtaacgtag ctatcctccg ccccggtaaa gcgagagacc agctcgccgg ttttgtccac
     4921 cagctggtca atctccacga aagaggatgc cgcgcgcgcg gtggcctgca tcacctccgg
     4981 cgcgacgctg gagacgccga gaatcgtcat cttgccgcag gcgttaatca cctgtttcag
     5041 tcccagttgt tgatagatat tctgcgtcat ggcttacagc actcccagca gcgagcagac
     5101 cacgctcaac gccacaatcg acagcagaat cgtggtgtaa cgcggaccct ttttcaccaa
     5161 atagaagtag atggcgaaca ctgccgccag cggcagcagg ccgggcgcga tggagtccag
     5221 aatctgctgt accaccactt cagagccttt cagtgcgcta atttttaaag gtgaggttat
     5281 cttgacaaag ctcgcggata gcgcgcccat catgataagc cccagtacgt tagcgccgta
     5341 gataagctcc ttgatgcgcc ctccctgcag cagactgata atcgaatcgc gccccagcgt
     5401 atagcccttg tgcaccatgc cgtagctgat tgccagcgta atcgccgggt agagaatcag
     5461 cggcaaaata ccgcccatcg cgctgccatt ggcggcgaac gggataaaaa tagcaatcaa
     5521 cagcggcatc accgccgccc agatgatgga gtcgcccatc ccggccagcg gccccatcag
     5581 gccggtttta atcccggtga tagaggcatc gttaatcgcc tcgccgcggg tcttttgctc
     5641 ttccatggcg atggagatcc cctggatcac cgcgccaaac gtctgctctg agttgaagaa
     5701 gttcagatgg cgttttaacg cctccacctg ctcctccttt tgcggataga gctttttgat
     5761 aatcggcgtc atcgaggcgc agaaaatcag gctctgtaaa cgctcatagg agctggaaac
     5821 ttccgcgccc agccagtaga tcaatcacgc tttggtgata tccgctttgg tcagcgcccc
     5881 ggactcccgc gcgcgctcaa ccagttcatg ctgcattacg tcgtcgctca ttatgccgct
     5941 ccttcatttt tcgccaggcc tttaatcagg aaggccacgc aggtgccgaa aatcgccatc
     6001 gccatgatgt ccaccttgag gtacagcacc gcgaagaatc cgccgataaa ccacggcagc
     6061 aggctctttt taccaatcac cataatggtg atggcgaagc ccagcgccgg gagaatcccg
     6121 cccatgattt caaacgagtg ggtcagccag tgcggcatca gcttgaggaa actttccact
     6181 acgttctggc cgaagtagtt ggcggcgaac accactggga aacgtagtac cagccccagc
     6241 agcgccgggt agagaaaggc gcagcgcata atggctgaca tattggccgt ttccgcgtgt
     6301 ttatcggcca tatgcaccca ggccgcattt agcgtgcgac gcaactggtc gaggaatacg
     6361 ccgataacgc caaatggaat cgccagtgca atcgccaggt tcgggtccat attggcctta
     6421 acggcgatag gaatggcgat acaggccgcc agcgccgggt ctgacggcac attgccgcct
     6481 ggcgttgagg tcacacccag atagaccaac tgcatccccg cgccgataat catcgccgca
     6541 ggcatattgc cgagcagcag gccgacaaag accgccacca ccactggctg taacagcatg
     6601 gcggagaagg tatagccaag gcgcaaccgg gcaaaccagt aatacagccc catcaggctt
     6661 gcaaaaagta aggtatccat aatttgttat cccatgttca gagggtcaga attttttcag
     6721 gatatcgtcc agcgactgcg gtttatcttc cgggatggtc tggaaaacag ccttaatgcc
     6781 gcgacttttc aaatcgttaa gaatgccaac gtctttttca tccagcgtta tgttctggaa
     6841 caccgcttta cggtttggcc cgccgcctaa accgccaacc tgaatatcag ccacgtcaaa
     6901 gccctgctgc accgcttcct gaatcgccgc cagcgacggg aacagcacca gcacgttgcc
     6961 gtcgcccagc tggttctctt tccacgcggc cgcgaagctc tggttgctaa agcagtccac
     7021 tttgatattc ggcggcgcgg ccatcaggta gatatttttc ataaacggat cggcgtccag
     7081 ctcgtcgctg accaccgcaa tacggttggc ctgagactgc cctacccact tcgtcaccac
     7141 ctgtccgtgg ataagacggc tgtcaatgcg acataacacg atgtttgcca tacgattttc
     7201 cttactgttg taattgacga atatgggaga cgacgtccag gcaactgttg cgcccagctt
     7261 ccaccagatc ggccacgcag gcggtcagtg ggccatgttc gcgcttgtcc agcgcctcca
     7321 gcagcagcga ggcattaagc ccggagatca ccgccaccgg ataatcggcg ctcagccgcg
     7381 ccgccacgtt agaggtggtg ccgccgataa aatcggtcac gataagcgaa ccttccggca
     7441 tggttttcac cacggcttcg acgcgctggt aaaactcgcc cagcgtatcg acgggcatca
     7501 gcgcgatttc cgtcacgcct tcgatttcgc ccatcaccat gcgcagactg ttgcagagct
     7561 gcagccccca gccgccgtga gttagcagca gaatttcagg taacggatga ataggtacag
     7621 ttgtggtcaa aatagcctcc tttgctccag tgtttacatt tcatcaggag caaaggacgt
     7681 gccagaattt gtgtcacgca cagaggaaat gagattggat ggggcgttaa gccccgtaaa
     7741 ccaggggatc agctgtagag cagttcgtag atataaaaat attctgcgtc cgataaacgg
     7801 atagcgtaag cctcttcaat cggcaaaaag gaggatttga tgacgctaaa ggcacgagcg
     7861 tccagatcaa gccggttttc tagcgccatc tgtaacggtt tgcggttaat cacgatacgc
     7921 tcgaccatgc agcagcagtg gatcagaaaa cgtagtgtca cctggcggct cggtttcagg
     7981 gagagcgtgt tggttaagta gttcagaaca tcctcgatct ctttcagaat tcgctgcggg
     8041 ttgagcaccg aaatatggtt gatgatgctc tccatggtca gcgcgctgat aaagcgcatg
     8101 gcgctgcgct ccatctccag tcggcgctcg ctgcttgaga gatccggcgt cagcagactg
     8161 agcaccagct ccggcccttg ctcggaaaac agttcctcca gcgagataaa cggtatatcc
     8221 ggcagtcccg gctggaaggt gccgacgatc cctgccagcc gttcactggc attaagcgcc
     8281 tgctgaatac gctccaggct gcgcacctcg ttatagtcga gaatcaccat acgcgtgtcc
     8341 tgcgacatca gctcgccaaa gctctcctcc agcacctttt tgatcttttc cgccgtcccc
     8401 attccggtta tacatgagat aaccagcact ttgccaccgc tctcttgctg cggcgtgcag
     8461 agccgacaag ggatattttt actctgcata agcacagcca attgcgatag atcgccagtt
     8521 tcgtagctta aatccagccc aacctccagc aggctggtca gagtgatatt tggcaacaac
     8581 agaacgtcaa tctggaacag cttgctgatg gtgctgccaa aatggaccag cgatccgata
     8641 tccaccatca gtatcagccg ctgccagcac cggtcctgaa tcatccgggt cagggtttcc
     8701 agggtgtcat gcaccgactg ctcaaatggc atatcaattg cgtagaacag atcgcgctcc
     8761 agcacccggt tcacatattg cgcctggctc gttgcggtgg tcgcagcgtg agcgattaga
     8821 atcacgccgc aatccgggct ggtgtcgatg cgctggcggt agtggcgaca ctctttcagg
     8881 aacaggcaca gccagaccac ctccgtcgcc gggcactgaa tatgcaacaa ctcattgatt
     8941 ttgcggcaca gaagcgtggc attgtcatac tcatctttac agcgatccag aatcaggctg
     9001 gatgaataga gttgaggtat aagccctcgc cgcacatagc caatcagcgc aagaaagtgt
     9061 ttacgtaacg ggttcgccag gttttctggc aatgtaaatc ctaatacctg ctccacgtaa
     9121 ccgatcagca gcgtcacacg ctcctcaatt tgatcgccat agcgcggtgg atgcgccgca
     9181 ctatggcggc tataaagacc atattcaaaa atcgaactga gtttgttttt caggatagcc
     9241 agcgtttctg cgggcggcac gttactgttg cgcagattga catattcacg agtcaggaaa
     9301 ctgtaaaaca ggtcgctctc ttctatttcc gcgctggtcg ccaacgaatt ttttaacgcg
     9361 ggcaacgtac gcgcatccac gttcagacga tctttaccgc caaacaacgc atcgaccagc
     9421 agacgctgct ctggcgtggt attaaacgac atctccgcca gtcgtttatc taactctagc
     9481 gtatcattac gctcggtcat tcccgacgcc cacgcctgcg cgcacaagaa ctgaatatca
     9541 cttttaagct ggccgatatt gccttccagg ggtttactta acaaccaatg caagagcgtt
     9601 ttatcaaggc ttatggtacg ttcaatttta cgactttcac gctgaaagaa gcttacaatc
     9661 aattcgagct gctcctcgac cgaacgctgg cgaatacccg gtaagtcaat acatacctgg
     9721 atacgccgct ggaaggtacg caatagtgcc gagttgaccg actctgtagt ggcgcaaata
     9781 aggcgcaccg aaattgaata tgttggccca cttgaaccaa gtgggcggta ttcaccttta
     9841 tcgagaatag aaaagagctt ctcttgtcct tcgtacggga ggcgatgcac ttcatccaga
     9901 agtaaataac ctccgtcagc ttgctccacc aatcccgttt tattctcact tgccccggta
     9961 aatgccccct tatggtgacc aaacagatgt gaggaaagca actccggatt atgagcatat
    10021 tcggcacagt taaagtaaac caacggaggt ggcgaaccta tggtgtgctt gcaagcgaag
    10081 caatgcatta actcagcaaa gaatgttttg ccaacaccag atgcaccggt taacagcaca
    10141 tgtagtccgt ggggataaag caccgctgcg cggccttttt ctaccgcatc acggagacta
    10201 cggtcgtatc cgataagtcc ggaaaacgga tcatcatctg tatatttttc tttctttggc
    10261 aataaactag ccactgaatg aactatgcat tcgcaatcat ccagttttcg attaatgagc
    10321 aattctagtg cgtgacgatg tagaaaaaaa acaggacgtc cctgactttt taccaccagg
    10381 ccttcattcc ataattgatt aagatcttta ctaaccgagt tgcgtgccag cccaaggtta
    10441 aaaccaatcg cctcagcagt aaatgctttc tccttcgcta aatcagacag ttccagccca
    10501 tacgtcagcc gctccagctc cttgtgaata acatcaatcc gtctcatttc tttaccgctt
    10561 aaaaaacaat agttgttcag aacatacact atttgtaaac gtaagaatga gaactatcgg
    10621 gaagaattaa tgttccttgt ggcaatctgg tttctgaata ttcgtgtttt aaatattact
    10681 tatggtctga aacaaccagg caggtgaaca gagctaagaa aaagtagcgt agaaatataa
    10741 aaacatatat cgcactgatg aatgtttccc ggcatgacta tgcacaccgg gaaatattta
    10801 gtaaattact cgcccttttt cgccgcctga atatacagca tctccagcgc cagcgtcgcc
    10861 gcagccagag cggtgatttc cgactgatca tatgccggag ccacttccac tacgtccatc
    10921 ccaacgatgt tgagatcttt caggccgcgt accagtttaa tggcgcgatc ggaggtcaga
    10981 ccgccaatta ccggcgtacc ggttcccggc gcaaatgcag gatccaggca gtcgatatca
    11041 aaggtcaggt aaaccggcat atcacccaca atttgtttca cctgggcaat aacgtcatcc
    11101 acgctgcgat cgttcacctg gcaggcgtcc agcacggtaa agccgttgtc tttatcaaat
    11161 tcggtacgaa taccaatctg cacggaatga ttcggatcga tcagaccttc tttcggcgcg
    11221 gtatagaaca tggtgccgtg gtcaaattca caaccgttcg cgtaggtatc ggtgtgggcg
    11281 tcaaagtgta ccagcgccat tttgccgaaa tgcttcgcat gagcacgcag cagcggcagc
    11341 gtaacaaagt ggtcaccacc gaaggagagc atacgcttac cggcagccag cagcttctcg
    11401 gcgtgcgcct gcagcttctc gctcatctca cgggcatcgc cgaaagcata taccagatca
    11461 ccgcagtcca cgacgttcag acgctcacgc atgtcgaaat tccacgggaa gcggttgtgt
    11521 tcccaggcca gattcgtcga aacctgacgg atagctgccg gaccgtggcg tccccccgca
    11581 cgaccagaag tggccatatc gaacggcaca ccagtaatca cccagtcagc atcgctgtca
    11641 tacggctgga agttcatcgg caggcgtaaa aaaccaaagg cattggaaac cagtgagtta
    11701 tcgtattgat gacctaaggt gctcatggat ggactcctat tacaaagtcg atataaaaaa
    11761 acccttccgc gtcgttaggc ccgacgagga agggttggat ttgcgaaaca caagtttgtg
    11821 ctaattatcg ccttaaatgg gcgaggcatc aagcgaggtt gctatttccg acacggtggc
    11881 ggcgcatagc cattgttatc tgctggaaaa gcttgtccag agcacggtgg cagcgcgttt
    11941 tgcgccgggc cttgtaattc aaaggcggca aagccgatta ccccggtatt acgcacatcg
    12001 ccttgcgccg aattggctgc gtaggaatca acaacgttgg cgaactgaaa agccgcttcg
    12061 gtatgtttat ctttgcggaa tcctttaatc gctaacgaat ctccggcatt cacaatatag
    12121 ccgttgttat tcagcgaacc ttgcttgccg ttcagcacat ccaggccatc aaccgtagca
    12181 acgatctcgt agttggtgtt acgactgtaa ttacgaacat ataattgata attcattccg
    12241 actctgacgt ggacaatata gcgtggttca ttattggatg aatcataaac acgcgtaata
    12301 ggtagagaat taaaattacc atcgcgaata gcatattcca gttcgttaat acgaatgcta
    12361 taaacgcggt catagccgga aggaacatta gtcgaataac taataacgat catctctgca
    12421 ggatcgcgcc aggctcgttc agcagatacg ccctggaccg atgagtgcac atccccaccc
    12481 caggcggtgc cgacattgcc cgatgcactg ctattatact gcgcattact ggaacaaccg
    12541 gccagcagca cgattaatgc caccaacgcg ctacgtactt tccatttttt cataaccgtt
    12601 ccctctgcat ttaattaatg cagcgattgt agggagatta tttaataaga aaagcagcca
    12661 gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata aatcgacccg
    12721 ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca aacctgcctc
    12781 gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct tcacttgatc
    12841 gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca tgtccgccac
    12901 ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca cgaacacgtc
    12961 aaccgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct gatatgcgcc
    13021 gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca ttggcattgt
    13081 cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt cacaggtaat
    13141 atccagcagc acagcgcggc gttccggcac ttgatccagc ccttccagcg gcagaaccgg
    13201 gaacaactgg tcgatccccc acgcatccgg catcgactgg aacagcgaga agttgacgta
    13261 cattttgtct gccatacgtt cctgcagctc gtcgataatc ggacgatgag cacggttttg
    13321 cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt ctgcccatgc
    13381 acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt gcagatccat
    13441 ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt gcatctcctg
    13501 ccaggtttcc cacatgcttt gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac
    13561 cgtatattcg ttacgttcca cgccgatgat attcgatacc agcacggtgt gatgcgcagt
    13621 taccgcacga cccgattcgg tgattaccgt cggatgcggc agaccatttt cttcacacgc
    13681 atcaccaatc gcccagatga tgttgttggc gtattcattg aggccatagt ttacggagca
    13741 gtcagactgc gaacgggtgc cttcatagtc cacgcccaga ccgccgccga cgtcgaagca
    13801 ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc
    13861 tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca gtagttgcag
    13921 gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc
    13981 caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc cctgcgaggc
    14041 cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt catccagcac
    14101 aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca tcttctcgcc
    14161 aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga tgacgctacg
    14221 ggtcatgcca gcatgtgcca gcaccgccat caactctgct ttggaaccgg cttccagacc
    14281 cagcggttcg cccgaatgaa tcagggactc gatcacgcgg cggtgctggt taactttgat
    14341 cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac gtttgaacgc
    14401 ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag
    14461 acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc gagcttccgg
    14521 gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat agtagttatt
    14581 gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg agctcattgc
    14641 aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca aacccataga
    14701 catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca gttaacgact
    14761 atcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag cagtgtatgc
    14821 tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac ataaccgaac
    14881 gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag agcgcccttg
    14941 ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag atgggtataa
    15001 catcggcagg tatgcaaagc agagatgcag agtgcgggga acgaatcttc accagaacgg
    15061 tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca cggccgcctg
    15121 ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg gcaacacgaa
    15181 agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc acaatggcag
    15241 aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt tccagccacg
    15301 tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta
    15361 aaatagccat ccagatgtta atccatccat accgattaac actcagactg ccagtgtttt
    15421 taacttgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac agtttgagct
    15481 aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg tccgagtccg
    15541 tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt ttagacgcga
    15601 tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg
    15661 ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag atcacccgta
    15721 acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct aactcctgtg
    15781 cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg
    15841 atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca actaatgaaa
    15901 ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag cgtcaggctg
    15961 aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt
    16021 tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc actcagcact
    16081 ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc atcaagccaa
    16141 ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg accggtcgtt
    16201 ttgttatcgg tggcccgatg ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata
    16261 cctacggcgg catggcacgt cacggtggcg gagcattctc tggtaaagat ccatcaaaag
    16321 tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc
    16381 tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa ccgacctcca
    16441 tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact ctgctggtac
    16501 gtgagttctt cgacctgcgc ccatacggtc tgattcagat gctggatctg ctgcacccga
    16561 tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg tgggaaaaaa
    16621 ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct
    16681 ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg ataggcattc
    16741 acgccgcatc cggcaaaaag ctgcccgcac aataacatca ttcttcctca acacgtttta
    16801 tcgcaaatta tcatcactac tgaaaccgat tacaccaata acaccagcca aatatttgta
    16861 atattttcat attattattc agttttcaca gttgttacat ttcttttcag taaagtctta
    16921 attgcagata acagcgttta atctatgatg atataactca attattttca tgcacttaaa
    16981 ccataactaa gataaatgtt agtgtaagcg attacactga tgtgatttgc ttcacatctt
    17041 tttacgtcgt actcacctat cttaattcac aataaaaaat aaccatattg gagggcatca
    17101 tgcctgacgc taaaaaacag gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc
    17161 ttgccgctct ggcgggatta ctctttggcc tggatatcgg tgtaattgct ggcgcactgc
    17221 cgtttattgc agatgaattc cagattactt cgcatacgca agaatgggtc gtaagctcca
    17281 tgatgttcgg tgcggcagtc ggtgcggtgg gcagcggctg gctctccttt aaactcgggc
    17341 gcaaaaagag cctgatgatc ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg
    17401 ctgcgccaaa cgttgaagta ctgattcttt cccgcgttct actggggctg gcggtgggtg
    17461 tggcctctta taccgcaccg ctgtacctct ctgaaattgc gccggaaaaa attcgtggca
    17521 gtatgatctc gatgtatcag ttgatgatca ctatcgggat cctcggtgct tatctttctg
    17581 ataccgcctt cagctacacc ggtgcatggc gctggatgct gggtgtgatt atcatcccgg
    17641 caattttgct gctgattggt gtcttcttcc tgccagacag cccacgttgg tttgccgcca
    17701 aacgccgttt tgttgatgcc gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag
    17761 cgaaacgcga actggatgaa atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc
    17821 tgtttaaaga gaacagcaac ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa
    17881 tgcagcaatt caccgggatg aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg
    17941 cgggttatac caacactacc gagcaaatgt gggggaccgt gattgtcggc ctgaccaacg
    18001 tacttgccac ctttatcgca atcggccttg ttgaccgctg gggacgtaaa ccaacgctaa
    18061 cgctgggctt cctggtgatg gctgctggca tgggcgtact cggtacaatg atgcatatcg
    18121 gtattcactc tccgtcagcg cagtatttcg ccatcgccat gctgctgatg tttattgtcg
    18181 gttttgccat gagtgccggt ccgctgattt gggtactgtg ctccgaaatt cagccgctga
    18241 aaggccgcga ttttggcatc acctgctcca ccgccaccaa ctggattgcc aacatgatcg
    18301 ttggcgcaac gttcctgacc atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt
    18361 atgcggctct gaacgtactg tttatcctgc tgacattgtg gctggtaccg gaaaccaaac
    18421 acgtttcgct ggaacatatt gaacgtaatc tgatgaaagg tcgtaaactg cgcgaaatcg
    18481 gcgctcacga ttaatctcct caggcttcct cccatcgcgg aggaagccac ctcttgcagt
    18541 catcttttct tcgctctatc ctctgccgct atgaaaacat cccgtctccc tatcgccatc
    18601 cagcaggccg ttatgcgccg cctgcgggaa aaactcgccc aggccaacct gaagctaggg
    18661 cgtaactacc cggagccaaa actctcttac acccagcgcg gaacctccgc cggaacagcc
    18721 tggctggaaa gctatgaaat ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct
    18781 tttattgaag aagtggtacc gcacgaactg gcacatttgc tggtatggaa acatttcggc
    18841 cgcgtagcgc cacatggcaa agagtggaag tggatgatgg aaagcgtgct gggcgttccc
    18901 gcccgtcgta cgcatcagtt cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc
    18961 tgcaagtgcc aggagcatca gcttaccgta cgccgccata atcgcgtagt tcgtggcgag
    19021 gccgtctatc gctgtgttca ctgcggtgaa cagctggttg cgaaataacc atctgaacta
    19081 tcaggaactt tcctgatctg gctgattgca taccaaaaca actttcgcta cgttgctggc
    19141 tcgttttaac acggagtaag tgatgtaccg ttatttgtct attgctgcgg tggtactgag
    19201 cgcagcattt tccggcccgg cgttggccga aggtatcaat agtttttctc aggcgaaagc
    19261 cgcggcggta aaagtccacg ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa
    19321 ctggcagggc aaaaaaggcg ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa
    19381 tgaaaaccga gccagccgcg tagagtggga acacgtcgtt cccgcctggc agttcggtca
    19441 ccagcgccag tgctggcagg acggtggacg taaaaactgc gctaaagatc cggtctatcg
    19501 caagatggaa agcgatatgc ataacctgca gccgtcagtc ggtgaggtga atggcgatcg
    19561 cggcaacttt atgtacagcc agtggaatgg cggcgaaggc cagtacggtc aatgcgccat
    19621 gaaggtcgat ttcaaagaaa aagctgccga accaccagcg cgtgcacgcg gtgccattgc
    19681 gcgcacctac ttctatatgc gcgaccaata caacctgaca ctctctcgcc agcaaacgca
    19741 gctgttcaac gcatggaaca agatgtatcc ggttaccgac tgggagtgcg agcgcgatga
    19801 acgcatcgcg aaggtgcaag gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg
    19861 aaagagctaa cctacactag cgggactctt tttgttaacc cctaccccac gcgtacaacc
    19921 gcgtggggag acgacgcgga tttttaacta tgcgtatccc ccgcatttat catcctgaac
    19981 cactgaccag ccattctcac atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg
    20041 tactgcgcat ggggccgggg caggcgttgc aattgtttga cggtagcaac caggtctttg
    20101 acgccgaaat taccagcgcc agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga
    20161 tcgacgatcg cgaatctccg ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa
    20221 aaatggaatt tacgatccag aaatcgatcg aactcggtgt aagcctcatt acgccacttt
    20281 tttctgagcg ctgcggcgtt aaactggata gtgaacgtct gaacaagaag ctccagcagt
    20341 ggcagaagat tgcaattgct gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc
    20401 gcccagcgat ggatctggaa gcctggtgtg cagagcagga tgaaggactg aaactgaatc
    20461 ttcacccgcg cgccagtaac agcatcaata cgttgccgtt accggttgaa cgcgtccgcc
    20521 tgctgattgg cccggaaggc ggtttatcgg cagatgaaat tgccatgact gcccgctatc
    20581 aatttactga tatcctgttg ggacctcgcg ttttgcgtac agagacaact gcgctcaccg
    20641 ccattaccgc gctacaagta cgatttggcg atttgggcta acggagaaga ataatgatca
    20701 agctcggcat cgtgatggac cccatcgcaa acatcaacat caagaaagat tccagtttcg
    20761 ctatgttgct ggaagcacag cgtcgtggtt acgaacttca ctatatggag atgggcgatc
    20821 tgtatctgat caatggcgaa gcccgcgccc atacccgcac gctgaacgtg aagcagaact
    20881 acgaagagtg gttttcattc gtcggtgaac aggatctgcc gctggccgat ctcgatgtga
    20941 tcctgatgcg taaagacccg ccgtttgata ccgagtttat ctacgcgacc tatattctgg
    21001 aacgtgccga agagaaaggg acgctgatcg ttaacaagcc gcagagcctg cgcgactgta
    21061 acgagaaact gtttaccgcc tggttctctg acttaacgcc agaaacgctg gttacgcgca
    21121 ataaagcgca gctaaaagcg ttctgggaga aacacagcga catcattctt aagccgctgg
    21181 acggtatggg cggcgcgtcg attttccgcg tgaaagaagg cgatccaaac ctcggcgtga
    21241 ttgccgaaac cctgactgag catggcactc gctactgcat ggcgcaaaat tacctgccag
    21301 ccattaaaga tggcgacaaa cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc
    21361 tggcgcgtat tccgcagggg ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg
    21421 aacctcgtcc gctgacggaa agtgactgga aaatcgcccg tcagatcggg ccgacgctga
    21481 aagaaaaagg gctgattttt gttggtctgg atatcatcgg cgaccgtctg actgaaatta
    21541 acgtcaccag cccaacctgt attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg
    21601 gaatgttaat ggatgccatc gaagcacgtt tacagcagca gtaacccacc ttagcgagaa
    21661 ggatctcgtt gagactctga gtgacagcgc ccttctttcc acgcatactg ggcgctgttg
    21721 cttttttgaa ccaggaaaca gaacctctga caatgaattt acagcatcac tttcttattg
    21781 ccatgcctgc tctccaggat ccgattttcc gtcgttccgt ggtctacatt tgcgaacata
    21841 ataccaatgg tgcaatgggg atcatcgtca acaagccgct ggaaaatctc aaaattgaag
    21901 ggattctgga aaagctgaag atcacgccgg agccgcgtga tgaatcaatc cgtctggata
    21961 aaccggttat gctcggcggt ccgctggctg aagatcgcgg gtttattttg catactccgc
    22021 cctccaattt tgcttccagc attcgcattt cagacaacac agtgatgacc acttcccgcg
    22081 atgtgctgga aacgctcggc accgataaac aaccgtctga cgtattggtg gctctgggtt
    22141 atgcctcctg ggaaaaaggt caactggaac aagaaattct cgataacgcg tggctaacgg
    22201 ccccggcgga tctgaatatt ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg
    22261 caaaactgat tggtgtggat attctcacca tgcctggtgt ggcagggcac gcctgatgag
    22321 tggaacctta ctcgccttcg acttcggcac caaaagcatt ggcgtagcgg tcggccaacg
    22381 cattaccggc actgctcgcc ctttgcctgc aattaaagca caggacggta cgccggactg
    22441 gaacctcatc gaacgtttac tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc
    22501 gctgaatatg gacggcaccg agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg
    22561 tattcatggt cgtttcggtg ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga
    22621 agcccgttcc ggtctgtttg aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga
    22681 ctctgcctct gcggttatta ttctcgaaag ctatttcgag cagggatatt aaggcgatct
    22741 aaaagcctgg cggatagtcg ttaccgccat cttgccggat gcggcgtgaa cgccttatcc
    22801 agcacacatc tggcagcggc tgtaggtctg ataagacgcg ttagcgtcgc atcagacatt
    22861 tattgccttt ataggcccta taaacttgcg catcgggcat ggcaacgtca caaacgccct
    22921 tcccccaccc gctgctgata actctgctga aacgttaaca tccccacctg ctgcccggtt
    22981 tgaataacat gcggtaactg gtgggttttc ccttcgcgaa tcaaattccc caccgcggga
    23041 gtgttaatca ataattcaaa cagcgccacg cgtccttcct gtttatccac ttccagcttt
    23101 tgtgacaaca cggcccgtaa actacctgcc agttgattac gtacgggatc tttttcctgc
    23161 gccggaaatg aatccaccag tcgctcaact gcctgcgcgg caccacgcgt atgtaatgtt
    23221 gccagcacca aatgcccggt ttctgccgcc gtcagtgcca gacggattgt ctcgctgtca
    23281 cgcagctctc cgagcaaaat cacatcagga tcttcccgca atgcggcccg caatcccgat
    23341 gcgaacgtca tacagtgcaa accaatttcc cgctgctgga tcaaacatcg ctggctggca
    23401 tagatatatt caacaggatc ttccagcgtc agaatatgcg catcggcatg ttgattgaga
    23461 tagccaacca tcgccgccag cgtggtagat ttgccgctcc ccgtcgcccc cgtcaccaga
    23521 atcaggccat tctcgctctt gagtaattcc ggcaataccg ttggtgcgcc aagctgttcg
    23581 agctgtggac agtgcgaagg taacaaccgt aatgccagag aaataccttg ccgttgcgcg
    23641 aacgcactgc cacgcaaccg ctggttttcc gccagcgaca cggcaaaatc cagctggcca
    23701 ttttccagca atattgcccg ctgatcgtca tccagccact cccgcagtag ctcttcgacg
    23761 tccggcgcat caaacggcgc agcttccatt agcccgcgaa tgcgccatcg tgcgggccag
    23821 gcgctgcaca ggtgtagatc cgagacgtta tgctttacac taagggccac aatttcttcc
    23881 atattcatac taagatcctc ggaaaatgaa cgatattgcg cataacctgg cacaggtccg
    23941 ggacaaaatc tcagcggctg caacgcgttg cggccgttct ccagaagaaa ttacgctgct
    24001 tgcagtcagt aaaacaaaac ctgcgagcgc catcgcagaa gccattgatg ccgggcagcg
    24061 tcaatttggt gaaaactacg ttcaggaagg ggtagataaa attcgccact ttcaggaact
    24121 gggcgtaaca ggattagaat ggcattttat tggcccgttg cagtctaata aaagccgcct
    24181 ggtggcagag catttcgact ggtgtcatac catcgaccgt ttgcgcattg ctacccgcct
    24241 caacgatcag cgcccggcag aacttccccc tcttaacgta ctgattcaaa ttaatattag
    24301 tgatgaaaac agtaagtccg ggattcaact ggcagaactg gacgagctgg cagcggcggt
    24361 cgctgaacta ccgcgtttac gtctgcgcgg gctgatggca atccctgcgc ctgagtcaga
    24421 atatgtaagg cagtttgaag ttgcacgcca aatggctgta gcatttgccg gactgaaaac
    24481 gcgctacccg catatcgaca cgctctctct gggaatgtcg gacgatatgg aagccgccat
    24541 tgcggcaggt agcacgatgg ttcgtatcgg cactgcaatt tttggtgcgc gtgattactc
    24601 taaaaaataa ggaattaaag gaacgccatg aatacgttga ctttcctgct ttcaacggtc
    24661 attgagctgt ataccatggt gctgttatta cgcatctgga tgcagtgggc tcattgtgat
    24721 ttttacaacc ccttctcaca gtttgtagtg aaggtaacac agccaattat cgggccactg
    24781 cgccgcgtta ttccggcaat gggtcccatt gatagcgcct cgctgctggt tgcgtacatt
    24841 ctcagtttta tcaaagccat cgtgctgttt aaagtggtga ccttcctgcc aatcatctgg
    24901 attgccggtt tactgattct gctgaaaacc atcggcctgc tgattttctg ggtcctgctg
    24961 gtgatggcga ttatgagctg ggtaagccag ggtcgtagtc cgattgaata cgtgctgatt
    25021 cagctggccg atccgctgct gcgcccgatt cgccgcctgc taccggcaat gggtgggatt
    25081 gatttctcgc cgatgatcct cgttctgctg ctgtatgtca tcaatatggg tgtcgcagaa
    25141 gtattacagg caaccggaaa catgctgctg ccggggctgt ggatggcgtt atgagtgccg
    25201 taacagttaa tgatgacggt ctggttttac ggctctatat tcagccgaaa gccagccgtg
    25261 attctattgt cggtttacat ggcgacgaag ttaaagtcgc cattaccgcg ccgccggttg
    25321 acggccaggc caacagccat ctggtgaagt ttctcggtaa gcaattccgg gtagccaaaa
    25381 gccaggtggt aattgaaaaa ggcgaacttg gccgccacaa acaaattaaa atcattaatc
    25441 cgcaacaaat cccgccagaa atcgcggcgt taattaatta ggtatcctat gcaaaaagtt
    25501 gtcctcgcaa ccggcaatgc cggtaaagtg cgtgagctgg cgtcgctgct tagcgacttc
    25561 ggtcttgata tcgtggccca aacagacctc ggcgtagatt ccgctgaaga aaccggcctg
    25621 acctttatcg aaaacgcgat tctgaaagcg cgccatgcgg caaaagtgac cggtttaccg
    25681 gcaattgccg acgactctgg tctggcggta gatgcgctgg gtggcgcgcc gggtatttac
    25741 tccgcgcgtt attccggtga agacgcgacc gatcaaaaga atctgcaaaa actgttggaa
    25801 acactgaaag acgtaccgga cgaccaacgt caggcgcgtt tccactgcgt gctggtatat
    25861 ctgcgtcacg cggaagatcc gactccgctg gtgtgccacg gtagctggcc gggcgtgatt
    25921 actcgtgaac aagcgggcac tggtggcttt ggttatgatc caatcttctt cgtaccttcc
    25981 gaagggaaaa ccgctgccga actgacccgc gaagaaaaga gcgccatttc ccaccgtggt
    26041 caggcattga aactgctgtt ggacgcttta cgtaatggtt aaattaccgc cgctgagtct
    26101 ctacattcac atcccgtggt gcgtgcagaa atgcccgtac tgcgatttca actctcacgc
    26161 gttgaaagga gaagtgccgc acgacgatta cgttcagcat ctgctttgcg atctggacaa
    26221 cgatgtggct tacgctcagg gccgtgaagt aaagacaatc tttattggcg gtggtacgcc
    26281 gagcctgctt tccggcccgg cgatgcaaac gctgctggac ggcgtgcgtg cgcgtttgcc
    26341 gctggcagcg gatgcagaaa ttactatgga agcgaaccct ggtacggtag aagccgatcg
    26401 ctttgtcgat tatcagcgtg ctggtgtgaa ccgcatctct attggtgtgc agagttttag
    26461 cgaagacaag ctgaaacgac ttgggcgtat tcatggcccg caagaagcga aacgcgcggc
    26521 aaagctggcg agcgggctgg ggctgcgtag ctttaacctc gatttgatgc atggactacc
    26581 ggatcaatca ctggaagagg cgcttggcga tctacgccag gccattgaac tgaatccgcc
    26641 gcatctttcc tggtatcaac tgaccatcga acctaatacg ctgtttggtt cacgcccgcc
    26701 ggtactgccg gacgacgacg cgctgtggga tatattcgaa caggggcatc agttattaac
    26761 cgcagcgggt tatcagcaat atgaaacttc cgcttacgcc aaacccagtt atcagtgcca
    26821 gcacaatctc aactactggc gctttggcga ctacatcggt attggctgcg gcgcgcacgg
    26881 caaagtgacc ttcccggatg ggcgcattct gcgtaccact aaaacgcgtc atccgcgtgg
    26941 ttttatgcaa ggaaggtatc tggaaagcca gcgtgatgtc gaagccgcag ataagccgtt
    27001 tgagttcttt atgaatcgct tccgtctgct ggaggccgcg ccgcgcgtgg agtttattgc
    27061 gtataccggg ctttgcgaag atgtgattcg cccacagtta gacgaggcga ttgcccaggg
    27121 ttatctcaca gaatgtgcgg attactggca gataacggaa catgggaagc tgtttttaaa
    27181 ttcgctgctg gagctttttc tggctgagta aacttgtatt gccggatgcg gcgtgaacgc
    27241 cttatccagc ctacatgtgg caacggttgt aggtctgata agacgcgcaa gcgtcgcatc
    27301 agacgttgat tgccggatgc ggggtcaacg ccgcatcctg ctacaaatcg tgcactatat
    27361 caaacttact taatcacccc attgcgccgc agttgttcgg tatctttttt ccagcgttca
    27421 ttagcttggt tgatatcgtc aattttcatc aaaatcgcga cttttttact gtttatttct
    27481 gaaactttca tcgttaacgg atattgccct ttttcgagcg ttacccagtc atcctgcaat
    27541 ttacggctga gtttttgtga ttgcagaaaa gtctgtcgcc gttgttcgtt gatatcgaaa
    27601 tctcgtttct gctgaatcgg cacttcataa cactcttcac tctgtttatg acatgccgca
    27661 atcgccggtt gataaacctc tttgtcatat ttaaccgcga acgcctcgct atcgttctgt
    27721 ttattaaact catcacgctg ttggtgaatt tttttaaagg cttcttcgcg ggtcatttgc
    27781 ttaccgtttg catcttttcc ccaatatgca tccgctgctt tctgcgccgc caccatttgc
    27841 ttatcaagcg ctttcacttc cttttccagc gcaggaatcg agttcttctg ggtattcaac
    27901 gcagattcaa gctgactaaa gcgataattg aaatcttcgc tattcacgat cagatattta
    27961 ttattcgttt tgatatcgtc gaccacccgc ccacgatcgc tgactgccgc ctgaaaagag
    28021 taaaagttca ccgaccagcc agacgcgggc gttcctttac tggttaacat cgccgagaat
    28081 tttaccggtt tatctttcgt ccaggtctgt tcgagcagtt cgtaatctgc caactgaccg
    28141 acccaggtat agagatcgtc actggaagag acatcgcctt ccgctgacca ggtggcctga
    28201 ttacccttag catcaagatt ttttaaggtg atagcatcaa gcttgagaat gccgtgatac
    28261 tgctttttga attgatcttt taagatattt tcagttggcg gttcgccatc tgcccaggca
    28321 ttaccagtca tcaacataag cagtgccgtg ccaacaatcc attgcttttt cacccgaaaa
    28381 acctcacgcg gaataattta ttccggtgat tatcattagg gtaatgaaaa ttggcaatgg
    28441 acgtaagtct gaagtgaaaa agccccggca cgataccggg gcgaggtgat tagtactgat
    28501 tgaagatctg ctggatctgc tgcggatctt tggtttgcgt cagagccagc tgcagcagga
    28561 cgcgcgcttt ttgcgggttc agcgtgccag aggcgacgaa gccgtatttc gcatcatcca
    28621 cttcagcatc ctgagtggta gcacccgtcg gtacgcggga agaacgcact actgcagtgc
    28681 cgtttttcgc ggcggttgcc agggtgtcga acacggattt atacaggtta ccattaccca
    28741 caccagcgct aacgatgcca tcatagcccg catctaccag tgctttagcc ggaagatcgg
    28801 atgcgttagc gtagttataa acgatgccga ctttcggcag ctcattcagc ttagagacat
    28861 cgaatggcgt atcgctggtg tgcttacgtg ccggggtacg ttggtagtca atcttaccgt
    28921 tgtgaatgta tcccagagga ccgtagttaa cagacttgaa ggtcgctacg tcggtggtgt
    28981 tggttttggt gacatcgcga ccgtccagta cggtgtcgtt catcaccacc agcacgccac
    29041 gattagcgga ggctttatca gctgcggtca ctaccgcgtt atacaggttg aatggaccgt
    29101 ctgcgctcat ggacgtggac gggcgcattg cgccgaccat caccaccggt ttgtcgcatt
    29161 tcaccgtcag gtcgaggaag taagcggttt cttccatcgt gtcggtaccg tgggtaatga
    29221 cgaagccgtc ggttttatcg cagtcggtgt taattttttt cgccagtgtc agccagacat
    29281 tatcgttcat gtcctgggag ccgatattca ctacctgctc gcctttaacg ttcgcaatgt
    29341 cttttagttg cggcaccgca ttaaccagat tttctacgcc aactttaccc gctgtgtagt
    29401 tagatttggt tgcggagtca ccaccaccgg caatggtccc gccggttgct aaaatggtga
    29461 tattgggtaa tgccaatgct gcaccactaa aacccataac cagtgcggca agtgccgtct
    29521 ttttgaaaaa ctccatttca ttcctccagt tacgtgaacg ctacgcatta tcccttagct
    29581 ctgtatggga aatttgacgt taaacaattt acaacgtgaa tatattttgg agatctacaa
    29641 agttagaggc aggtaacaaa acgaagaatt aaacggcata aaaaagtatt atgccgtctt
    29701 aaaatagagg attattttaa attcccgacc agggctttgc ggctatcttc cagagtcaca
    29761 acgcggctac aaacatcttt gccaaactgc tggaaatctt tttcctgctt tttccactcg
    29821 gtttggattg aggattgcag cccccccagg cttcccagca cattctgtaa tgggttaccg
    29881 ccgcttttca acaccgcttt cgcgcccatt tcattaatgc tgtcctgtaa aattccaccc
    29941 attgcctgat tcactaattg ctggccttcg gcgcgaacct gatcaatggc tttatagtga
    30001 aacgtcaggc catcgctgcg cgtttcgata atgcggttca tctgctcttt cagctgcgca
    30061 tcaagtttgg tcagacggct gcgcattttg ctgctttcgc ccatctcctg aacgataatt
    30121 ttatccagcg caatacgggc tttttcgacg cggcttttcg cgccttcatc aatccacggc
    30181 agcgtgctgc gtagttcagc ctgataatcc ttcgcctgct cgcgctgggc ggcattcagg
    30241 gaatattgct taccgttata catcacgttg ccgtctggcg tgatcaccag attgccgttt
    30301 tcgcccttca cctgcacggt ttgcgggctg acaatcacat cgtcacgcgg cgtgacgctg
    30361 cactggtagt cggcgtgagc ggtcattgcc gtcactgaaa gtgctgccgc cagcagcatt
    30421 ttgcgcatca tagtcttccc tcaagaaaaa atcaggccag catttgctgg ccccagattg
    30481 ataacaaagt acgcgttttc catgccggat gcggcgtaaa caccttatcc cgcctactcg
    30541 atcgtgcatc tttccaggcc tgataagcgt agcgcatcag gcagttttgc atttgtcatc
    30601 gcccgtatgc tttcttagtc ccaccaaacg tcgaaaagtt cgctggtgcg tacctcatcc
    30661 agtttgcgct cttccagcca cttacgcaca atcgcctgat gttcttcggt gcatttgccg
    30721 atttcctgca tgcagatcag accttcccag gccagataac cgctgccgtc aaaggccagt
    30781 ttgttcggtt cgataacctc gttaataaaa tcatcaacgg ttttatcaat ctgttcttcc
    30841 gatgtacctt ccgggaatcg ccatgccacc gaaaatccta attcctggaa ttcgtcgatg
    30901 tgcatttttt tacgcagacg acggctacgg ttctttgcca ttatttcacc ctctcgaaca
    30961 ttaagtccca tactccgtga ccaagacgat gaccacgttg ttcaaatttc gtcaccggac
    31021 gtgatgccgg acgcggtacg taatcattgc tctctgacag gtttttataa ccgtcaatag
    31081 aagacatcac ttcaagcata tgttccgcat aaggttccca gtcggtcgcc atatggaata
    31141 cgccccccag ctgcagtttg ctttttacca gttcggcaaa cggcacctgt acgatacggc
    31201 gtttattatg gcgcgctttg tgccacgggt cagggaaaaa gagctgcacc atgcgcaatg
    31261 aattgtcagg aatcatttta tgcagcactt caaccgcatc gtgacacatc acgcgcaggt
    31321 tgcttaaacc ttcttcatgc gcagaagcca ggcacgcacc aacgcccggt gaatgcactt
    31381 caatgccgag gaagtcctgc tcagggcgat ctttagccat tgccaccagc gacgccccca
    31441 tgccaaaacc aatctcaagc gtcaccggcg cttcacggcc aaaaagcgcg gggaaatcca
    31501 gcatatcttc gctgaactca acgcccatca ccggccagta gttttccagc gcatgttcct
    31561 ggcctttggt cagtcgcccc tggcggcgca caaaactacg gatacggcgc agtgggcggc
    31621 cgttttcatc aaattccggt gaaatgacgt cgtttttcat aaagatttag tcgcttgtga
    31681 aagtgttctg aaaacgggca ttatccaaag ttagttgccg gatgcaagca tgataaggcc
    31741 gtggctgcgg aaagttccgg tttacaccct gccgtcgctg tgctgcaatc ttgcccccaa
    31801 caacagtgaa ttcggtgacc atgcaagcgt cgcaattttc agcccaggtt ctggactggt
    31861 acgataaata cgggcggaaa acgctgccct ggcaaattga caagacgccc tacaaagtat
    31921 ggctctcaga agtgatgttg caacaaactc aggttgcgac cgttatcccc tattttgaac
    31981 gctttatggc gcgcttcccg acggtgaccg atctcgccaa tgcgccgctc gacgaagttc
    32041 tccacttgtg gaccgggctt ggctattacg cccgcgcgcg caacctgcat aaagcggcac
    32101 aacaagtggc gaccttacac ggcggtaaat tcccggaaac ctttgaagaa gtcgcggcgt
    32161 taccgggtgt cgggcgttcc accgcaggcg cgattctctc gctttctctg ggtaagcact
    32221 ttccgattct cgacggtaac gtcaaacgcg tgctggcgcg ctgctatgct gtaagcggct
    32281 ggcctgggaa aaaagaggtc gagaataaat tatggagttt aagcgagcag gtgacgcccg
    32341 cggtcggcgt ggaacggttt aatcaggcga tgatggattt gggcgcgatg atttgtacgc
    32401 gctcgaaacc gaaatgttcg ctctgtccgc tacaaaacgg atgtattgcc gccgccaaca
    32461 atagctggtc gctttatccg ggcaaaaaac cgaaacagac gctgccggag cgcaccggct
    32521 actttttgct attacagcac gaagatgaag tattgctggc gcagcgtccg ccgagcggat
    32581 tgtggggcgg tttatactgt ttcccgcagt ttgccgacga agaaagtttg cggcattggc
    32641 tggcgcaacg gcagattgct gccgataacc tgacgcagct gaccgcgttt cgccatactt
    32701 tcagccattt ccacttagat attgtgccta tgtggcttcc cgtgtcgtca ttcaccggct
    32761 gcatggatga aggcaatgcg ctctggtata acttagcgca accgccgtca gttggcctgg
    32821 cggctcccgt ggagcgtttg ttacagcagt tacgcactgg cgcgccggtt tagcgcgtga
    32881 gtcgataaag aggatgattt atgagcagaa cgattttttg tactttcctg caacgtgaag
    32941 cagaaggtca ggattttcag ctgtaccccg gcgagctggg aaaacgcatc tataacgaga
    33001 tctccaaaga agcctgggcg cagtggcagc acaaacaaac catgctgatt aatgaaaaga
    33061 aactcaacat gatgaatgcc gagcaccgca agctgctgga gcaggagatg gtcaacttcc
    33121 tgttcgaggg taaagaggtg catatcgagg gctatacgcc ggaagataaa aaataaaaac
    33181 agtgccggag cacgcctccg gcagcttgca taaaaacaaa cacaacacgc acccggaatg
    33241 atgaaaaaat atctcgcgct ggctttgatt gcgccgttgc tcatctcctg ttcgacgacc
    33301 aaaaaaggcg atacctataa cgaagcctgg gtcaaagata ccaacggttt tgatattctg
    33361 atggggcaat ttgcccacaa tattgagaac atctggggct tcaaagaggt ggtgatcgct
    33421 ggtcctaagg actacgtgaa atacaccgat caatatcaga cccgcagcca catcaacttc
    33481 gatgacggta cgattactat cgaaaccatc gccgggacag aacctgccgc gcatttgcgc
    33541 cgggcaatta tcaaaacgtt gctgatgggt gacgatccga gttcggtcga tctctattcc
    33601 gacgttgatg atattacaat ttcgaaagaa cctttccttt acggtcaagt ggtggacaac
    33661 accgggcagc cgattcgctg ggaaggtcgc gcgagcaact tcgcggatta tctgctgaaa
    33721 aaccgtctga agagccgcag caacgggctg cgtatcatct acagcgttac cattaacatg
    33781 gtgccgaacc accttgataa acgtgcgcac aaatatctcg gcatggtccg ccaggcgtca
    33841 cggaaatatg gcgttgatga gtcgctgatt ctggcgatta tgcagaccga atcttccttt
    33901 aacccatatg cggtcagccg ttccgatgcg ctgggattaa tgcaggtggt acaacatact
    33961 gccgggaaag atgtgttccg ctcgcagggg aaatccggca cgccgagccg cagtttcttg
    34021 tttgatcctg ccagcaatat tgataccggc accgcgtatc tggcgatgct gaacaatgtt
    34081 tatctcggcg gaattgataa cccaacatcg cggcgttatg ccgtcatcac cgcctataac
    34141 ggcggcgcag gcagcgtgct gcgagtcttt tcgaatgata agattcaggc tgccaatatt
    34201 attaacacca tgacgccggg cgatgtttat cagacgctga cgacccgcca tccctctgcg
    34261 gaatctcgcc gttatcttta taaagtgaat accgcgcaaa aatcctaccg ccgccgataa
    34321 ttccattaac cgcccctgac gatgctcagg ggcaaaaatg ttatccacat cacaatttcg
    34381 ttttgcaaat tgggaatgtt tgcaattatt tgccacaggt aacaaaaaac cagtccgcga
    34441 agttgataga atcccatcat ctcgcacggt caaatgtgct ttttcaaaca ctcatccgca
    34501 tcacgatgtg aggaaattaa catgaatctt aagctgcagc tgaaaatcct ctcttttctg
    34561 cagttctgtc tgtggggaag ttggctgacg accctcggct cctatatgtt tgttaccctg
    34621 aagtttgacg gtgcttctat tggcgcagtt tatagctcac tgggtatcgc cgcggtattt
    34681 atgcctgcgc tgctggggat tgtggccgac aaatggttaa gtgcgaaatg ggtatatgcc
    34741 atttgccaca ccattggcgc tatcacgctg ttcatggcgg cacaggtcac gacgccggaa
    34801 gcgatgttcc ttgtgatatt gattaactcg tttgcttata tgccaacgct tgggttaatc
    34861 aacaccatct cttactatcg cctgcaaaat gccgggatgg atatcgttac tgacttcccg
    34921 ccaatccgta tctggggcac catcggcttt atcatggcaa tgtgggtggt gagcctgtct
    34981 ggcttcgaat taagccacat gcagctgtat attggcgcag cactttccgc cattctggtt
    35041 ctgtttaccc tgactctgcc gcatattccg gttgctaaac agcaagcgaa tcagagctgg
    35101 acaaccctgc tgggcctcga tgcattcgcg ctgtttaaaa acaagcgtat ggcaatcttc
    35161 tttatcttct caatgctgct gggcgcggaa ctgcagatta ccaacatgtt cggtaatacc
    35221 ttcctgcaca gcttcgacaa agatccgatg tttgccagca gctttattgt gcagcatgcg
    35281 tcaatcatca tgtcgatttc gcagatctct gaaaccctgt tcattctgac catcccgttc
    35341 ttcttaagcc gctacggtat taagaacgta atgatgatca gtattgtggc gtggatcctg
    35401 cgttttgcgc tgtttgctta cggcgacccg actccgttcg gtactgtact gctggtactg
    35461 tcgatgatcg tttacggttg cgcattcgac ttcttcaaca tctctggttc ggtgtttgtc
    35521 gaaaaagaag ttagcccggc aattcgcgcc agtgcacaag ggatgttcct gatgatgact
    35581 aacggcttcg gctgtatcct cggcggcatc gtgagcggta aagttgttga gatgtacacc
    35641 caaaacggca ttaccgactg gcagaccgta tggttgattt tcgctggtta ctccgtggtt
    35701 ctggccttcg cgttcatggc gatgttcaaa tataaacacg ttcgtgtccc gacaggcaca
    35761 cagacggtta gccactaatt acgcaaagaa aaacgggtcg ccagaaggtg acccgttttt
    35821 tttattctta cttcaacaca taaccgtaca accgtttcat gccatccgca tcggtttcgc
    35881 tataaacacc ttgcagctcc ggcgaaaatc ccggcaacaa attcacccct tcttccagtg
    35941 caaggaaata acgttgaacc gccccacccc agacttcccc gggtaccacg caaagcacgc
    36001 caggtggata aggcaacgcc ccttctgccg caattcgccc ttcggcatca cgaatccgca
    36061 ccaactccac ttcaccgcga atataagcgc tatgcgcatc ctgggggttc atcaccactg
    36121 acgggaaact ctgctggcgg aacatcgctt tttgtaggtc tttgacgtcg aaactgacat
    36181 acagatcgtg catctcctga cacaactggc gcaaagtgta gtcgcgatag agcaccggat
    36241 atttgttata aacgctcggc aacacctcag ccagcggcga gtcatcctca atatgctgtt
    36301 caaactgcgc caacatctct accagttgtg ccagcttctc gtggctttcc gccggagtta
    36361 ataagaacag aatggagttg agatcgcact tctccggcac aatgccgttc tcacgcagat
    36421 agtgcgccag aatcgtcgcc ggaacgccaa agtcgctata ttcgccggtt tcggcattga
    36481 tacctggtgt agtgagtaac agcttgcacg gatcaacaaa atactgatcc gcggcatatc
    36541 cttcaaagcc gtgccacttc gcccccggct caaaactgaa aaaacggcgg tcgctggcta
    36601 acactgatgt cggataatcc tgccacaatt tgccatcaac aacgggcggg ataaacgggc
    36661 ggaacagctt acagcgcgca agaatagcct tgcgcgactc aatacccaac tcaacgcact
    36721 cagcccacag ccgacgccca ctctcccctt catgaatttt ggcgttaaca tccagtgcag
    36781 caaacagcgg atagaaaggg ctggtagaag catggagcat aaaggcgtta ttcaaccgct
    36841 tatgcgggca aaaacgcgcc tgtccgcgga tatggttatc ttttttatgg atctgcgacg
    36901 tctgtgagaa tcccgcctgc tgtttgtgca ccgactgagt cacaaagatc cccggatcgt
    36961 tttcgttaag ttctaatagc agcggcgagc tatccgccat catcgggata aactgttcgt
    37021 aaccgaccca cgcggaatca aacagaatgt aatcacacag atgcccaacg gtatcgatca
    37081 cctgacgggc gttatagaca gttccgtcat aggttcccag ctgaataatc gccaggcgaa
    37141 acgggcgcgg cagctcggct ttttctggcg caacgtcgcg aatttgctgg cgcagatact
    37201 cttcattaaa acagtgcgca tcaataccgc caatgaaacc aaacgggttg cgtgaagctt
    37261 ccagatagac cggcgtcgcc cccgcctgaa tcagcgcgcc gtgatgattc gacttatggt
    37321 tgttacggtc gaagagcacc agatcgccac gcgttaacag cgcattcgtc accactttat
    37381 tcgctgccga tgtgccgttc agcacaaaat aggttttatc ggcatgaaag actttggctg
    37441 cgaatttctg cgcatctttc gccgatcctt catgaataag caaatcgccc aattttacgt
    37501 cagcgttaca catatcggcg cgaaagacgt tctcaccaaa gaaatcgtaa aaatggcgtc
    37561 cggcaggatg ctttttaaag aacgcaccat gttgatgtcc agggcaagca aaggtgctgt
    37621 tgcccatttc aacgtactgc gtcagcgtgt cataaaacgg tggcagcaaa ttctcttcat
    37681 actgacaggc tgcggattcc agctccagcc actgctgctc gttgccgttg attaccgccg
    37741 taacgcccgc aggtaattca acagcatgtt cggaatacaa aaacaccggt agatgaaaac
    37801 cggtgcgctt aagcaacgca agaatgccac tgcgactatc cgcagcggta atgacgactg
    37861 ccgcgacgtc cgtaaaatca gtatctccca acgccaccac gcggcgatga gtagaaagtc
    37921 gggataccag ttcactactg gcggcaatat tcattgattt cataagcgca aacccgtttc
    37981 ggggaagtaa gaataccgga caaggtggaa aaccctgccc catgagatat gggtcaaact
    38041 ggtcaccagc tccgaccgcc agacatcagt aaaagcagaa acgctctgat tttactgttg
    38101 tcctgcagtg agcgtgcgtt aacttcaccg catgagcagt aacatagaaa gggaaacgtt
    38161 tcgcgcgaaa cggcgataag cgagagaatg taaggagatg gcgtgcatcg ggcaaactcc
    38221 gtacagagag gagaaaattc gcgcaatcat ggcacctttc gctaaagcgt gcaagccagg
    38281 actttgcgaa caaacaagcc atcgacggca ccaaacggtc ataataagaa aatcaaacaa
    38341 tacagttaac aggaactctt gtggtcatcg gcccttttat caacgctagt gcagtattac
    38401 tgggtggcgt tctcggcgca ctgctcagcc aacgcttacc ggaacgtatc cgcgtctcca
    38461 tgacatctat ttttggtctg gcatcgctgg ggattggtat tttactggtg gtgaaatgtg
    38521 ccaaccttcc agcgatggtt ttagctacgc tacttggcgc tctaattggc gaaatttgtt
    38581 tgctggagaa aggtgtcaat acagcagtca ccaaagcaca aaatctgttt cgtcactcac
    38641 gtaagaagcc agcgcatgaa tcttttattc agaattatgt cgcgattatt gtcctgtttt
    38701 gcgccagcgg caccgggatc ttcggggcga tgaacgaagg gatgaccggc gatccgagta
    38761 ttttaatcgc caagtcattt cttgatttct ttacggcgat gatcttcgcc tgctcgctgg
    38821 gtattgcggt atcggtgatt agtatcccat tactgatcat ccagttaacg ctggcgtggg
    38881 ctgccgcgct gatattaccg ctgaccacac cggcgatgat ggcagacttc agcgccgtag
    38941 gcggtttatt gctgctggca accggattac gcatctgtgg cattaaaatg ttcccggtgg
    39001 tcaacatgct tccagcactc cttctggcaa tgccgctttc cgccgcctgg accgcctggt
    39061 ttgcctgaca atgcgtgcaa tagcggcaaa gtgatgatag attgtgcagt ctgcagtaaa
    39121 ttgaagaaat ttgattgacg agacgaggcg aatcaggttt aatgcgcccc gttgcccgga
    39181 tagctcagtc ggtagagcag gggattgaaa atccccgtgt ccttggttcg attccgagtc
    39241 cgggcaccaa attcatatca acggacctcc acggaggtcc gtttttcgtt tcagaacacc
    39301 atgatttaag cgttctacct acaaatcaat tctaccgaac tcaaccagat tccccccaca
    39361 tcaaccccat tgtgtgggta taattgcggg tataccccag ttcgacagaa tttgtaccct
    39421 ctttaacgca ttcagaaggc tgtactatgg cactgactga cgcaaaaatc cgagctgcaa
    39481 agccctctga caaggcttat aaactcactg acggggctgg catgttcctg ctggttcatc
    39541 ctaatggttc ccgttactgg cgtctccgtt atcgtattct gggtaaggag aagactctgg
    39601 cacttggtgt gtatccagaa gtttctctct ccgaagctcg tacaaaacgg gatgaggccc
    39661 gaaaactgat ttcagagggg attgaccctt gcgaacagaa aagagctaaa aaagtagtcc
    39721 ctgatttaca actctctttt gaacatattg cacgacgctg gcatgccagt aataaacaat
    39781 gggcacaatc acacagcgat aaggtactca aaagcctcga aacacacgtt ttccccttta
    39841 tcggcaaccg ggatatcaca acactcaata ccccggacct gcttatccct gttcgtgctg
    39901 cagaagctaa acaaatttat gaaatcgcca gtcgtctgca gcaaagaata tctgctgtaa
    39961 tgcgttatgc tgtacagtct ggcatcatca gatataaccc ggctctggat atggctggtg
    40021 cattgaccac ggtaaaacgc cagcatcgcc ctgctctgga tctttcacgc ctgcctgaac
    40081 ttctgtcgcg tattgacggt tataaaggcc agcctgtcac ccgactggca gttatgctga
    40141 atttactggt ttttattcgt tccagtgaac tcagatacgc ccgctggtct gaaattgata
    40201 ttgacaatgc catgtggact attccagccg aacgcgaacc tctgccaggc gtaaaattct
    40261 cacaccgggg ctccaagatg cgaacaccac atcttgtgcc actcagcaaa caggctgtag
    40321 ccatactgac agaacttcag acatgggcag gtgaaaatgg tctgatattt acgggtgcac
    40381 atgacccgcg tcaaccaatc agtgaaaata ctgtaaataa agccctgagg gtgatggggt
    40441 atgacacacc ccaggaagtc tgtggccatg gattccgggc gatggcgtgc agtgcattga
    40501 ttgaatcagg tttgtggtcc cgcgatgctg tggaacgtca gatgagccat caggaacgta
    40561 acggtgtacg tgctgcttac attcataaag cagaacatct ggaagaacgc cgcttgatgc
    40621 tacaatggtg ggccgatttt ctggatgcaa acagagaaaa atttatcagt ccatttgaat
    40681 atgcaaagat taataatcca ttaaaacagt aatcatcccg ggcaaatgcc cgggaaccta
    40741 ctccaggatt attttctttg ttaaaaaaac agacaaaacg gtattaac
//
LOCUS       DAZKIV010000024        39265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZKIV010000024 DAZKIV010000000
VERSION     DAZKIV010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.024"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..762)
                     /gene="aqpZ"
                     /locus_tag="ACHOT6_001320"
     CDS             complement(67..762)
                     /gene="aqpZ"
                     /locus_tag="ACHOT6_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFP9946565.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKYAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            1188..2846
                     /gene="ybjD"
                     /locus_tag="ACHOT6_001321"
     CDS             1188..2846
                     /gene="ybjD"
                     /locus_tag="ACHOT6_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFP9946566.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTLRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2843..3799)
                     /gene="ybjX"
                     /locus_tag="ACHOT6_001322"
     CDS             complement(2843..3799)
                     /gene="ybjX"
                     /locus_tag="ACHOT6_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFP9946567.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGNFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3950..5065
                     /gene="macA"
                     /locus_tag="ACHOT6_001323"
     CDS             3950..5065
                     /gene="macA"
                     /locus_tag="ACHOT6_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFP9946568.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5062..7008
                     /gene="macB"
                     /locus_tag="ACHOT6_001324"
     CDS             5062..7008
                     /gene="macB"
                     /locus_tag="ACHOT6_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFP9946569.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADSLAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSIFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7081..7305)
                     /gene="cspD"
                     /locus_tag="ACHOT6_001325"
     CDS             complement(7081..7305)
                     /gene="cspD"
                     /locus_tag="ACHOT6_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFP9946570.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7455..7529
                     /gene="yljB"
                     /locus_tag="ACHOT6_001326"
     CDS             7455..7529
                     /gene="yljB"
                     /locus_tag="ACHOT6_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFP9946571.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7629..7949
                     /gene="clpS"
                     /locus_tag="ACHOT6_001327"
     CDS             7629..7949
                     /gene="clpS"
                     /locus_tag="ACHOT6_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFP9946572.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7980..10256
                     /gene="clpA"
                     /locus_tag="ACHOT6_001328"
     CDS             7980..10256
                     /gene="clpA"
                     /locus_tag="ACHOT6_001328"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFP9946573.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(10600..10687)
                     /locus_tag="ACHOT6_001329"
     tRNA            complement(10600..10687)
                     /locus_tag="ACHOT6_001329"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10651..10653),aa:Ser,seq:gga)
     gene            complement(10941..11159)
                     /gene="infA"
                     /locus_tag="ACHOT6_001330"
     CDS             complement(10941..11159)
                     /gene="infA"
                     /locus_tag="ACHOT6_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFP9946574.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(11444..12148)
                     /gene="aat"
                     /locus_tag="ACHOT6_001331"
     CDS             complement(11444..12148)
                     /gene="aat"
                     /locus_tag="ACHOT6_001331"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFP9946575.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(12190..13911)
                     /gene="cydC"
                     /locus_tag="ACHOT6_001332"
     CDS             complement(12190..13911)
                     /gene="cydC"
                     /locus_tag="ACHOT6_001332"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFP9946576.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13912..15678)
                     /gene="cydD"
                     /locus_tag="ACHOT6_001333"
     CDS             complement(13912..15678)
                     /gene="cydD"
                     /locus_tag="ACHOT6_001333"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFP9946577.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15801..16766)
                     /gene="trxB"
                     /locus_tag="ACHOT6_001334"
     CDS             complement(15801..16766)
                     /gene="trxB"
                     /locus_tag="ACHOT6_001334"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFP9946578.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            17311..17805
                     /gene="lrp"
                     /locus_tag="ACHOT6_001335"
     CDS             17311..17805
                     /gene="lrp"
                     /locus_tag="ACHOT6_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFP9946579.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17940..21929
                     /gene="ftsK"
                     /locus_tag="ACHOT6_001336"
     CDS             17940..21929
                     /gene="ftsK"
                     /locus_tag="ACHOT6_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFP9946580.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQQVAPQPQYQQPQQPV
                     APQPQYQQPQQPVAQQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPV
                     DTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLA
                     RSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDI
                     AGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELS
                     VYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRM
                     MRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKAR
                     AAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDM
                     LYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGA
                     EELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNR
                     EVLAPPPFD"
     gene            22084..22695
                     /gene="lolA"
                     /locus_tag="ACHOT6_001337"
     CDS             22084..22695
                     /gene="lolA"
                     /locus_tag="ACHOT6_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFP9946581.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22706..24049
                     /gene="rarA"
                     /locus_tag="ACHOT6_001338"
                     /gene_synonym="mgsA"
     CDS             22706..24049
                     /gene="rarA"
                     /locus_tag="ACHOT6_001338"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFP9946582.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            24140..25432
                     /gene="serS"
                     /locus_tag="ACHOT6_001339"
     CDS             24140..25432
                     /gene="serS"
                     /locus_tag="ACHOT6_001339"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFP9946583.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDLAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            25671..28115
                     /gene="dmsA"
                     /locus_tag="ACHOT6_001340"
     CDS             25671..28115
                     /gene="dmsA"
                     /locus_tag="ACHOT6_001340"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFP9946584.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDTKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            28126..28743
                     /gene="dmsB"
                     /locus_tag="ACHOT6_001341"
     CDS             28126..28743
                     /gene="dmsB"
                     /locus_tag="ACHOT6_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFP9946585.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28745..29608
                     /gene="dmsC"
                     /locus_tag="ACHOT6_001342"
     CDS             28745..29608
                     /gene="dmsC"
                     /locus_tag="ACHOT6_001342"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFP9946586.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(29643..30269)
                     /gene="ycaC"
                     /locus_tag="ACHOT6_001343"
     CDS             complement(29643..30269)
                     /gene="ycaC"
                     /locus_tag="ACHOT6_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFP9946587.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDTPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            30583..31731
                     /gene="ycaD"
                     /locus_tag="ACHOT6_001344"
     CDS             30583..31731
                     /gene="ycaD"
                     /locus_tag="ACHOT6_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946588.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            31941..33370
                     /gene="ycaM"
                     /locus_tag="ACHOT6_001345"
                     /pseudo
     CDS             31941..33370
                     /gene="ycaM"
                     /locus_tag="ACHOT6_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
     gene            complement(33371..34279)
                     /gene="ycaN"
                     /locus_tag="ACHOT6_001346"
     CDS             complement(33371..34279)
                     /gene="ycaN"
                     /locus_tag="ACHOT6_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9946589.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            34379..34969
                     /gene="ycaK"
                     /locus_tag="ACHOT6_001347"
     CDS             34379..34969
                     /gene="ycaK"
                     /locus_tag="ACHOT6_001347"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFP9946590.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(35051..35791)
                     /gene="pflA"
                     /locus_tag="ACHOT6_001348"
     CDS             complement(35051..35791)
                     /gene="pflA"
                     /locus_tag="ACHOT6_001348"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFP9946591.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(35983..38265)
                     /gene="pflB"
                     /locus_tag="ACHOT6_001349"
     CDS             complement(35983..38265)
                     /gene="pflB"
                     /locus_tag="ACHOT6_001349"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9946592.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(38320..39177)
                     /gene="focA"
                     /locus_tag="ACHOT6_001350"
     CDS             complement(38320..39177)
                     /gene="focA"
                     /locus_tag="ACHOT6_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFP9946593.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9767 a         9560 c        10367 g         9571 t
ORIGIN      
        1 gatgaatcaa catataacaa cattttaaaa aaaaagcctg acattgcgcc aggccttctg
       61 cgttaattaa ttacgttttt ccagtagagt ccggtaaatc agaccaccga taataccgcc
      121 gacaattggc accacccaga agaaccacag ttgttctaat gcccagccgc cctggaagat
      181 agcaaccgcg gtgctgcgcg ccgggttaac agaagtgtta gtcaccggaa tactgattaa
      241 gtgaattagc gttaaggcca gaccaatggc aattggtgca aaaccagcag gagcgtattt
      301 gtcagttgca ccgtggatca ctaacaggaa gcccgcactc aataccagtt caactaccag
      361 cgcggaaagc atggaataac cgcctggtga atgctcgcca taaccgttag aagcaaaacc
      421 gctggctgcc gcgtcaaaac ccgttttacc actggcaatt aaatacagca gcgccgctgc
      481 aacaataccg ccgacaacct gggcaattac gtagccaacg acttcttttg ccggaaaacg
      541 tccgccagcc cataaaccaa tagtgaccgc cgggttaaaa tgaccaccag aaatgtgacc
      601 aacagcaaag gccatcgtca gaacggtcag accgaacgcc agcgccacgc cggcaaaacc
      661 gatgcctaat tccgggaagc ctgcggccag aacagcacta ccacagccac caaaaacaag
      721 ccagaaagta ccaaaacatt cagctgctaa ttttctgaac atatccacct caattaaaaa
      781 ttgaccctgt gaaaaatatg gtcgttttat agggtcgtcg taaaaaaatg acgacggaaa
      841 taatgcgcgg ctattttaaa aacgaaggcg agtcattcac cagataaata aatccagtaa
      901 atttgattta gggcaacagc gggttgcccc atatagtcat ttgtctgatt gacagtgtag
      961 tgcaagcaaa agatttaatc ctttaggcgt aataaaaaat aatttatcat gctaattatt
     1021 tgattttttt gtttttgcag acttatcagc aagagggagt ataacgcgat tattcgctca
     1081 tttttcagac atttgccatg cttaaatgtg atgtcatcac gtattagcaa ggcctttccc
     1141 gttatactgc cagcgtaaag gataagtcac atatttctgg aggggatatg attcttgagc
     1201 gcgttgaaat tgtgggtttt cgcggtatca accgtttgtc gttgatgctc gaacaaaaca
     1261 acgtcctgat tggggagaac gcgtggggta aatccagctt gctggacgcc ttaaccctgc
     1321 tgctatcgcc agaatcagat ctctaccatt ttgagcgcga cgatttctgg ttcccgccgg
     1381 gagatatcaa cgggcgagaa catcatctgc atattatttt gaccttacgc gaatcgctgc
     1441 caggccgaca tcgggttcgc cgttatcggc cgctggaagc gtgctggacg ccatgcaccg
     1501 atggctatca ccgtattttt tatcgtctgg aaggggagag tgcggaagac ggcagcgtga
     1561 tgacactgcg cagttttctc gataaagacg gacatccgat tgatgtcgag gatattaacg
     1621 atcaggcacg ccatctggtg cgtttaatgc cggtgctgcg cttgcgtgat gcccgtttta
     1681 tgcgccgtat tcgtaacggc acggtgccaa atgttcccaa tgtggaagtc accgcgcgcc
     1741 agctcgattt cctcgcccgt gagttgtcct cacatccgca aaatctctct gatggacaga
     1801 ttcgtcaggg actttccgca atggtgcagt tgcttgagca ttatttctct gagcaggggg
     1861 ctggacaggc gcgatatcgt ttaatgcggc ggcgggccag caatgagcaa cgaagctggc
     1921 gctatctgga catcatcaac cggatgattg accgacctgg agggcgctcg tatcgggtta
     1981 ttttgctcgg cctgtttgct actttattgc aggcaaaagg cacattgaga ctggataaag
     2041 acgcgcgtcc gttattgcta attgaagatc cagaaacccg cctgcatccg attatgcttt
     2101 ccgttgcctg gcatctgttg aatcttttgc ctttacagcg tattgctacc accaactctg
     2161 gcgagctgct ttcgttaact ccagtggaac atgtttgccg cctggtgcgt gaatcttctc
     2221 gtgttgccgc ctggcgtctg gggccaagtg gcttgagcac cgaagacagc cggcgcatct
     2281 cttttcacat tcgttttaat cgtccgtcat cgctgtttgc tcgctgctgg ttgctggtgg
     2341 aaggggaaac ggaaacctgg gttatcaatg aactggcgcg ccagtgtgga caccatttcg
     2401 atgccgaagg gatcaaggtc attgagtttg cccagtccgg gctaaagcca ctggttaaat
     2461 ttgcccgccg aatggggatt gaatggcatg tactggtcga tggcgatgaa gcagggaaga
     2521 aatatgccgc tacggtacgc agcctgttga ataacgatcg ggaagccgaa cgagaacatt
     2581 taacggcgtt accggcgctg gatatggaac attttatgta tcgccaggga ttttccgatg
     2641 tgttccaccg cgtggcgcaa atcccggaaa atgtaccgat gaatctgcgc aaaattatct
     2701 cgaaagcgat ccatcgctct tccaaacccg atcttgccat tgaagtggca atggaggccg
     2761 gacgtcgggg tgtggactcc gtaccgacgc tgctgaaaaa aatgttctca cgcgtgctgt
     2821 ggctggcgcg cggtcgcgcg gattaaccgc gaaacatcgt ggccatttgt ggctgaatag
     2881 cgtcgagcat ctcatagcgc cgacggtatt cagcccgttt tttactggcg atttcggcaa
     2941 tctcttttcg tgctatctgt gctggaaggc ggtaatggcg ttcagcatca catacgccgc
     3001 caaccgattc ccagaaagcg ttgtaatcgg catggatctt gccttcttta tcacgataac
     3061 gcaggctgcg gtaaatatgc gtttcattgc tgacggcaat aatctgctct acctgcaaac
     3121 gttgggcaaa cagacaggcc gcttccatca cgaggcgttt gggaaatagc ccgtggcagg
     3181 ctttcgtcgc attctggatt tcctgatgtg gaatttccca ttttgcgcct tgcagtccgc
     3241 caataaacat cgttcttttc ccctgatatt cacacagggt aaacgtgatc tctgccagag
     3301 gaataccttc gctgttgcga aacaggattg tgctgtcacc ttctttatcc attgagatca
     3361 tcatggtcag ctcaagcgtg aactgctcgc cgtttttgcc ttccagcttc gccagttgca
     3421 gcccgggggt attcaaatat aagctgaatt cttccgccga catacatccg cggagtaacg
     3481 cataatggta acgtaacgcc tccagcaatt gcttacggct aagattcgcc gcaaggtaag
     3541 ggcgatgcag acgcacaggc agtcgcggtt ggcgcgttaa caacacattg agattaggcc
     3601 agtgggaaag ttcgttcatc cactcaacgc ttaaacgcgg cataatcaac gagcgcagca
     3661 aaaatttctg gcgaaaacta cggcgatgcc agaagttacc cggccgacac tgtccacgtg
     3721 ccagactaag aaaaagtgac aggctgctga gagattcaga tggcgtaaag gtccgttcag
     3781 ttagctgcga catattcatg aaatcaatgg ttatacatga cgtcgatttc accattgcgt
     3841 atcttaacca aacatcaata gtgtgattac taacgtaaat tttatggttt tgttgatatt
     3901 tcgttgaagt taatgacccg gattggcata tggagtattc agaatattta tgaaaaagcg
     3961 gaaaaccgtg aagaagcgtt acgttattgc gctggtgata gtcatcgccg gactgattac
     4021 gttatggaga attcttaacg cacccgtgcc gacttatcag acactgattg tgcgccccgg
     4081 tgatttacag caaagcgtgc tggcgaccgg aaagctggac gcgctgcgta aggttgacgt
     4141 gggcgcgcag gtcagcggtc agttgaaaac gctgtcggtg gcgattggcg ataaagtaaa
     4201 aaaagaccag cttttagggg tcattgatcc tgaacaggct gaaaaccaga tcaaggaggt
     4261 cgaagcaacg ctgatggagc tacgcgcgca gcggcagcag gcggaagcgg agctgaaact
     4321 ggcgcgggtg acgtattccc gtcagcaacg tctggcacaa acgcaggcgg tttcacagca
     4381 ggatctcgac accgccgcga cggagatggc tgtgaaacag gcgcaaattg gcaccattga
     4441 cgcgcaaatc aagcgcaatc aggcttctct cgatacggct aaaaccaatc tcgattacac
     4501 tcgcatcgtt gccccgatgg ccggggaagt cacgcaaatc accactctgc aaggccagac
     4561 ggtgattgcc gcacaacaag caccgaacat tctgacgctg gcagatatga gcaccatgct
     4621 ggtaaaagcg caggtttctg aagcggatgt aatccacctg aagccggggc aaaaagcctg
     4681 gtttacggta cttggcgatc cactgacgcg ctacgagggg caaatcaagg atgtactacc
     4741 gacgccggaa aaggttaacg acgctatttt ctattacgcc cgttttgaag tccccaaccc
     4801 caatggtttg ctgcggctgg atatgactgc gcaagtgcat attcagctca ccgatgtgaa
     4861 aaatgtgctg acgatccctc tgtcggcgtt aggcgatccg gttggcgata atcgttataa
     4921 agtcaaattg ttgcgtaatg gtgaaacacg cgagcgtgaa gtgacgattg gcgcacgtaa
     4981 cgataccgat gttgagattg tcaaagggct ggaagcgggc gatgaagtgg tgattggtga
     5041 ggccaaacca ggagctgcac aatgacgcct ttgctcgaat taaaggatat tcgtcgcagc
     5101 tatcctgccg gtgatgagca ggttgaggtg ctgaagggca tcaccctcga tatttatgcc
     5161 ggtgagatgg tagcgattgt tggcgcttca ggttccggta aatcgaccct gatgaatatt
     5221 ctcggctgtc tggataaggc caccagcggc acctatcgcg tcgccggtca ggatgttgcc
     5281 acactggacg ccgattcgct ggcgcaactg cgccgcgagc atttcggctt tattttccag
     5341 cgttaccatt tactttcgca tttaaccgcc gagcagaacg ttgaagtacc cgccgtctat
     5401 gccggtcttg agcggaaaca gcggctgctt cgcgcccagg agttactgca acgactgggg
     5461 ctggaagacc gtacagagta ttatccggca cagctttcgg gtggtcagca acagcgcgtc
     5521 agcatcgcgc gggcattgat gaacggcggt caggtaattc ttgccgatga accaaccggc
     5581 gcactggaca gccattctgg cgaagaggtg atggcgatcc tgcatcagct gcgcgatcgc
     5641 gggcatacgg tgattatcgt cacccacgat ccgcaggtcg ctgctcaggc cgagcgggtg
     5701 atcgaaattc gcgacggcga aattgtgcgc aatcctcccg ccattgaaaa agtgaatgtt
     5761 gctggcggga cggagcctgt tgtcaacacg gtgtctggct ggcggcagtt tgtcagcggt
     5821 tttaacgagg cgctgacgat ggcatggcgg gcgctggcag cgaataaaat gcgtacttta
     5881 ctgaccatgc tggggattat tatcggtatt gcgtcggtgg tttccattgt cgtggtgggt
     5941 gacgccgcca aacaaatggt gctggcggat attcgttcta ttggtacgaa tactattgat
     6001 gtctatcccg ggaaagattt tggcgatgac gatccgcaat atcagcaggc gctgaagtac
     6061 gacgacttaa tcgccatcca aaaacaaccg tgggtcgcct cagccacacc tgccgtctcg
     6121 caaaacctgc gcctgcgtta taacaatgtt gatgttgctg ccagtgccaa tggcgtgagc
     6181 ggcgattatt ttaatgtcta tggcatgacc ttcagtgaag gaaacacctt taatcaggag
     6241 cagctgaacg gtcgtgcgca ggtcgtggtt ctcgacagta atactcgccg ccagcttttc
     6301 ccccataaag cagatgtggt tggcgaggtg attctggtcg gcaatatgcc cgccagagtc
     6361 attggtgtgg cggaagaaaa acagtcgata tttggtagca gtaaagtgct gcgtgtctgg
     6421 ctaccttaca gcacgatgtc cgggcgagtt atgggccagt cgtggcttaa ctccattact
     6481 gtcagggtga aagaaggatt tgacagcgcc gaggcggaac agcaactcac gcgtttactt
     6541 tcactgcgcc acggtaagaa ggatttcttt acctggaaca tggacggcgt cttgaaaact
     6601 gttgaaaaga ccacacgtac tttacaactg tttctgacgc tggtggcggt gatttcgctg
     6661 gtggtgggtg gtattggtgt aatgaatatt atgctggtgt cagtgaccga gcggacgcgg
     6721 gaaattggca ttcgcatggc tgtaggtgcg cgagcaagcg atgttttgca acagttcctg
     6781 atcgaagccg tactggtttg cctggtcggt ggcgcgttgg gaataacact gtcactgtta
     6841 attgctttca ccttgcagct tttcttaccc ggctgggaga ttggtttttc accgttggca
     6901 ctgctgctgg cgtttctctg ctcgacggcc accgggattt tatttggatg gttacccgca
     6961 cgaaatgcgg cacgactgga tccagtagat gctctggcac gagagtaatt tttgagataa
     7021 aaatgccagc cgatcgggct ggcattttgc ctttaggatg tacacaatga gacagaagag
     7081 ctatgcgact gccgcttcta cttcgacggg cacaataaca ctggcgtgat tgccttttgg
     7141 cccctggtgg acatcaaact gaacggattg tccagctttt agcgttctgt aaccatccat
     7201 ctgaatggtg gaataatgag cgaaaatatc ttcgccgccg ccttcagggc agatgaaacc
     7261 aaaccctttg gcattgttga accacttaac agtacccttt tccatgcttc gacatccttc
     7321 gcaaatctta tacaagtaag atggaataaa ccggggtcag agagggggct gttcaaaacc
     7381 tcgccaactc tagaaataca atttagagaa ttagggcgag ccgtcaagcg tttgacaggg
     7441 gccagggggc aggtatgaat caaaaatttg aagcagttaa cgctattgac aggaatgtga
     7501 cagatgtcgc tgatgccaac gatagatgat agttaatcta tcatgtggag tagattggtc
     7561 aggcaaataa gctcttgtca gcggcagggc gttctgccga taaccgtaac cgaagatgat
     7621 aactgacaat gggtaaaacg aacgactggc tggactttga tcaactggcg gaagaaaaag
     7681 ttcgcgacgc gctaaaaccg ccatctatgt ataaagtgat attagtcaat gatgattaca
     7741 ctccgatgga gtttgttatt gacgtgttac aaaaattctt ttcttatgat gtagaacgtg
     7801 caacgcaatt gatgctcgct gttcactacc aggggaaggc catttgcgga gtctttaccg
     7861 ccgaggttgc agaaaccaaa gtggcgatgg tgaacaagta cgcgagggag aatgagcatc
     7921 cattgctgtg tacgctagaa aaagcctgaa tgcaggcata aaaattgggg gaggtgccta
     7981 tgctcaatca agaactggaa ctcagtttaa atatggcttt cgccagagcg cgcgagcacc
     8041 gtcatgagtt tatgaccgtc gagcacttgt tactggcgct gctcagtaac ccatctgccc
     8101 gggaggcgct ggaagcgtgt tctgtggatt tggttgcgct ccgtcaggaa ctggaagcct
     8161 ttattgaaca aaccacaccc gttctgcctg ccagtgaaga ggagcgcgac acacagccga
     8221 cgctgagttt tcagcgtgta ctgcaacgtg cggtcttcca tgtccagtcc tccggtcgca
     8281 atgaggttac cggtgcaaac gttctggtcg ctatctttag cgaacaggag tcgcaggcgg
     8341 catatctgtt gcgtaaacat gaagtcagcc gtctcgatgt ggtgaatttt atctctcatg
     8401 gcacgcgtaa agacgagccg acacagtctt ctgatcctgg cagccagcca aacagcgaag
     8461 aacaagctgg tggggaggaa cgtatggaga atttcacgac gaacctgaat cagcttgcgc
     8521 gcgtgggcgg aatcgaccca ctgattggtc gtgagaagga gctggagcgt gctattcagg
     8581 ttctctgccg tcgccgtaaa aacaacccgc tgctggtggg ggaatctggt gtcggtaaaa
     8641 ccgcgattgc agaaggtctt gcctggcgaa ttgttcaggg cgatgtgccg gaagtgatgg
     8701 ctgactgtac gatttactct ctcgatatcg gttctctgtt agcgggcaca aaatatcgcg
     8761 gcgactttga aaaacgtttt aaagcgttgc tcaagcagct ggagcaggac actaacagca
     8821 tcctgtttat tgatgagatc cacaccatta tcggtgcggg ggcagcgtct ggtggtcagg
     8881 tcgatgcggc taacctaatc aaaccgttgc tctccagcgg taaaattcgc gtaattggtt
     8941 cgacaaccta tcaggagttc agcaacattt tcgagaaaga ccgtgctctg gcacgtcgct
     9001 tccagaaaat tgatattact gaaccgtcaa tcgaagaaac tgttcaaatc atcaatggcc
     9061 tgaaaccgaa gtatgaagcg caccacgacg tgcgttatac cgcaaaagcg gtgcgtgcgg
     9121 cggtagagct ggcggtgaaa tacatcaatg atcgtcatct gccggataaa gcgattgatg
     9181 ttatcgacga agcgggcgct cgcgcacgcc tgatgccggt aagcaaacgc aagaaaaccg
     9241 ttaatgtggc ggatattgag tccgtggtgg cccgtattgc acgcattcca gagaagagtg
     9301 tttctcagag tgaccgcgat accctgaaaa acctcggcga tcgcctgaaa atgctggtct
     9361 tcggtcagga taaagccatt gaggcgctga ctgaagccat taagatggcg cgtgcaggtt
     9421 taggtcacga acataaaccg gtcggttcgt tcctgtttgc cggtcctacc ggggtcggga
     9481 aaacagaggt gacggtacag ctttcgaaag ctttgggcat tgagcttctg cgctttgata
     9541 tgtccgagta tatggaacgc cataccgtca gccgtctgat tggtgcgcct ccgggatacg
     9601 ttggttttga tcagggcggt ttgctgactg atgcggtcat caagcatcca catgcggttc
     9661 tgctgctgga cgaaatcgag aaagcgcacc ctgacgtgtt caatattctg ttgcaggtga
     9721 tggacaacgg tacgctgacc gataacaacg gacgcaaagc ggacttccgt aacgtggtgc
     9781 tggtgatgac cactaacgcc ggggtacggg aaactgagcg caaatccatt ggtcttatcc
     9841 accaggataa cagcaccgat gcgatggaag agatcaagaa gatctttaca ccggaattcc
     9901 gtaaccgtct cgacaacatt atctggtttg atcatctgtc aaccgacgtg atccatcagg
     9961 tggtggataa attcatcgtc gagttgcagg ttcagctgga tcagaaaggt gtttctctgg
    10021 aagtgagcca ggaagcgcgt aactggctgg ccgaaaaagg ttacgaccgg gcaatgggcg
    10081 cacgtccgat ggcgcgtgtc atccaggaca acctgaaaaa accgctcgcc aacgaactgc
    10141 tgtttggttc gctggtggac ggcggtcagg tcaccgtcgc gctggataaa gagaaaaatg
    10201 agttgactta cggattccag agtgcacaaa agcacaaggc ggaagcagcg cattaatctg
    10261 attgttaggt aggttggtca agtccgtaat ctcgaaagag gttacggact ttttgtttat
    10321 ggggtggagg aggttcagac ccttttttta atgatgatgg taagttgttg ataattagtg
    10381 ctgcgggaag gtaaggataa aaaagggtgc tgcaggagaa tgggatggtt ttgctttatt
    10441 aacaacgggc taaacgtgta gtatttgagt tcactgccgt acaggcagct tagaaattca
    10501 caggtaacat actccacccg cccaccatgt tcactgccgt acagacagat aaaatgcgaa
    10561 aaaaaagctc gcactttcgt acgagctctt ctttaaatat ggcggtgagg gggggattcg
    10621 aacccccgat acgttgccgt atacacactt tccaggcgtg ctccttcagc cactcggaca
    10681 cctcaccaaa ttgttttgct accaaacctc atgggtggca acggggcgct actataggga
    10741 gttggagtaa aacggtcaag aagaatttta atgataatta ttgtttgctc atactgtaaa
    10801 caagttgtgc agtatatcta catcgagaca agttacggac ttatacttcc aaagtacttc
    10861 atacatatca caaaataaaa aggccggtta aaccgacctt ttactcgttc tttctcttcg
    10921 cccatcaggc ggtaaaacaa tcagcgacta cggaagacaa tgcggccttt gctcaggtcg
    10981 tacggggtca gttcaacagt cactttgtcg cccgtcagga tgcggatgta gtttttgcgc
    11041 attttaccgg agatgtgtgc agtaaccacg tgaccgtttt ctaactctac gcggaacatg
    11101 gtattaggca acgtttcaag aacggtacct tgcatttcaa tattgtcttc tttggccatc
    11161 taatcctctg gggtatcact accgtaattt gaaccggcaa gataatgccg aagttctgta
    11221 aataagtaaa gatttgcgcg ctaaatcgca acaaacaggt tcggcacatt actccgaaaa
    11281 cacacggcta agccgcacca aaagcgcaac gtataaggga gcggtgagat aaacgatggg
    11341 cgttacctga cgcgaaaaat tccttatcgg cagcggggta atgagcgtaa ccaactctgc
    11401 gaccgcaatt ataacactct ggggagaaat gtgccgaaaa cattcattct tgtggtgaaa
    11461 acaagcatcg tggtacccag aaattattcg gcaatcgtcc gaggcgcatt tgattgagat
    11521 aattaaggta atcccggcgg ggaatttcgc aggcaccaag cgatgctgtg tgatcgttaa
    11581 ggacctggca gtcgataagc ttaccgccat gaccgataaa ttcctcacag aataccagaa
    11641 gcgccgtttt agacgcattt tccatccggc tgaacatgga ctcgccacaa aatagcgttc
    11701 cctgggccac gccgtacata ccgccgacaa gctcatcttc acgccagact tcaatggagt
    11761 gggcatggcc gagttcgtga aggcgatggt aggcttcgac cacgccacgc gtgatccagg
    11821 ttccttcttc gcgatcgctg gcacagcctt caatgacctg accaaaagcg taattcatcg
    11881 tgacacgata gggcgagcgt ttatgaaatc gcttcatact acggctgata tgcagtgatt
    11941 ctggccatag caccgcgcgg ggatcgggcg accaccagag gatggggtcg cctggagaaa
    12001 accacggaaa aataccacgc tggtaagcca ttaacaggcg cgcagggcta agatcgcccc
    12061 caagtgccag caggccgtta ggctcacgta atgcgccttc cggggaaggg aaggctattg
    12121 aatggcgaga aagctgaacc aggcgcatga ccgcaaaact ccacgcaagt cggatcgttc
    12181 aataatagct tacaaaccct gcttgaactg gtaataacgc ccctgtctgg caagcagctc
    12241 tgcgtgagta ccttgctcaa taatttgccc gttgtccatc actattattt gttggaaacg
    12301 agagagtccg cgaagtcgat gggtaaccat taacaccgtt ttctcacgca tcatttctgc
    12361 aagcaattca aggatctggc tttcggttgt ggcatctaag ccttcggtag gttcatccag
    12421 caacaccagt ggcgcatcat gtaacagcgc acgggcgata gccagacggc gcagttcacc
    12481 accggagagc tggcgtccgc cttcacctaa ccaactgttg agacctgcat cctcgagcag
    12541 cttctccaga ccaacgcgac gcaagctctc cgccagagcc tcatcactac tgccaggcga
    12601 ggcgagtaac agattatcac gcagcgtggc gctaaacaga tgcactcgct gaggaacaac
    12661 gctgatggtc tgtcgtagag ccgcttcatt caggctggct atggggctat cgttaagcaa
    12721 aatctctccc tgttgcgggt cccaagcgcg ggtcagcagt tgtaacagtg ttgatttgcc
    12781 gcatccggtt cgcccgagaa tcgcaatatg ttccccggcg tttacctgaa gagaaatccc
    12841 tttaagtgcc tgttgggatt gctccggata agtgaactga acatcccgta atgtcagcga
    12901 aacgcgatcg gcaacacgag tttgggtatc agggaaggtg acttccggtt tttgatccgt
    12961 taaatcagtg atccgaacgg cagaggcaat gacttgcccc agatgctgaa atgcacccgt
    13021 tactggtgcc agtgcttcaa acgcggctaa cgcgcagaag acaaacaggg caattaacgc
    13081 gccgggttga gcattgccgc caacgccgcc agacgccatc cacagcatca ggatcaccgc
    13141 taacgcgcca atgagcagca ttatcgcttg cgacaatgcg gtcagttcag attgacggcg
    13201 ttgcgcttcc agccattgaa tttctgtatt ctctagttgc gtgcgataac gatcgctggc
    13261 accaaaaatg gtcagctcag cttgcccttg cagccaggcc gtcagttgtt ggcgatactg
    13321 tccgcgaaga tgagtcagat tttgcccggt gctttttccc gcacgataaa acagcggtgg
    13381 catcaggaaa agcgtcagta acataatgcc gcccagcgta aaggcgaggg tgaaatcaag
    13441 gaaacttaac ccgattgtca ccaccataat caccacaaaa gcgcccacca gcggcgagat
    13501 aacgcgcaga taaagatgat cgagcgtatc aacatccgcc accacgcgat tgagcaattc
    13561 gccctgacga tagcgcgcca gtccggcagg ggagaggggc agcaatttgc tgaaggtgta
    13621 aatgcgcaga tgctgcaaca cgcggaaagt cgcgtcgtga cttaccagac gttcaaaata
    13681 acgcccggca gtacgggtga ttgctgcgcc acgcacgccc gcagcgggta gcatatagtt
    13741 gaagctgtac agtccggcaa cccccgcaac cgctgaggcc gagaggaacc agccggaaag
    13801 tgtcaacaga ccgatactgg cgagcagcgt gacaattgcc agcacgatac caagacttaa
    13861 catccattta tgacgtttat acagtgccag atagggtagc aaagcgcgca tttaaatctc
    13921 ctcctgacga tgggccagta atgtggcgaa tgggccacca gccacactta attccgcgta
    13981 acgtccttgc tcaataatcc ggccatcctg cataacccaa atgacatccc agtcagcaag
    14041 atcttctaac tggtgggtga ccattaacgt tgtctggcgc agagaggcgg cattcagcgc
    14101 ctccattacg cgctgttcac tgtgagcatc aaggctggca gcgggttcat ccaacagtaa
    14161 tagcgaacag ggatttagta acgcacgggc caccgccacg cgctgcgcct gccccacgga
    14221 aaggcgggca gcctggtcgc caacaggtgt atcaacgcct tgtggcagga gcggtagaaa
    14281 ctcgctgacc caggcgttat ccagcgctgc ttgtaattct tgttcgctgg catcaggtcg
    14341 cgccagtagt acgttatccc gcaatgttgc tgccggtaat tgtgggtttt gcccaaccca
    14401 ggagagatgt ttacgccatg attctggtga taaatcgcgt aattctatcc cgttgattcg
    14461 taatgatccc tgatatgaga gaaaaccaga aagcgcgttc agcagtgagc ttttacctga
    14521 accgctgcga ccaaccaaca ctgcacgttg gcctgctggc aaagtaaagt tcagcggtcc
    14581 ggccagcgtt ttaccttccg gcgacgtgat aaacagatcc tcggcctcaa tggtcaccgg
    14641 atcggtcgat gctaattcca cctcgccacg ctgcggatgg gcgagcgggg tttccataaa
    14701 cgttttcaga ctgtcagctg caccaacagc ctgggcttta gcatgataaa acgtaccgag
    14761 atcgcgtaat ggctggaaaa actctggcgc aaggatcagg gccagaaaac ccgcagccag
    14821 cgtcacgcca gtatcgtagt gaccaaaatc cagctcgccg agataggaaa aaccaaagta
    14881 gaccgccacc agagcaattg acagcgaggt aaaaaattcg agaatgccgg aggataaaaa
    14941 tgccagccgt agcacttcca ttgtccgttg gcggaaatct tccgaagcag aacgaatact
    15001 ttcaatttca gcttcaccac gaccaaaaat acgcaatgtt tccatgccgc gcaggcgatc
    15061 gaggaaatgc ccacttaagc gagcaagagc gagaaagtta cgtcggttag catcggcagc
    15121 ccccattcca accagcgcca taaacaacgg aattaacggt gcagtgccca gcagaatgag
    15181 cgccgcagcc cagtttgagg ggaagattgc caccacaatc agcaacggca ccgacactgc
    15241 cagcgccatt tgcggcaggt agcgtgcata gtaatcatgc atatcgtcaa tttgctcgag
    15301 taccagcgtc gcccagctcc ccgcaggttt accctgaatc cacgctggcc ctgcttgttg
    15361 cagacggtcg agaacctgcc ggcggatggc aaagcggata tgctgcccgg cgtgataacc
    15421 cacccgttcg cgtaaccaga ccacccatgc gcgcagtaca aaggtcagaa ccagtaacgt
    15481 aaagggaagc agcagggctt cacggggaat attctccata atcatatgtt gcagaattcg
    15541 cgccatgaac caggcctggg caatgatcaa tatgccgctc acaaagccca gcagacgaga
    15601 aatattcagc caacgttggg agatgacgct ttgctgtttt aaccagcggg ttaactcttt
    15661 ttgacgagat ttattcattg cacgcttagc aggtgagtta tcagaattat ttgcagagca
    15721 atgttacaac ggggaaaaaa taaaggcgac ccatagtcgc atggtgtcgc cttctttact
    15781 tttgttactg atttgtaaaa ttattttgcg tcagctaaac catcgaggta gcgttccgca
    15841 tcaagtgctg ccatgcagcc tgtaccggcc gaagtaatgg cctgacgata aatatgatcc
    15901 atcacgtcac ccgcggcaaa gacgccaggg atgctggtct gggtggcatt accatgaata
    15961 cccgactgta ctttgatgta gccgttttcc agttccagct gcccttcgaa aatggcagta
    16021 ttcgggctgt gaccgatagc aacaaacaga ccggcaacgt cgagtgactc gatgttatcg
    16081 ctgttttgcg tatcgcgcag acgaacgcca gtgacaccca tttgatcgcc ggtcacttct
    16141 tccagcgtac ggttggtgtg cagaatgatg ttgccgttct ccactttatc catcagacgc
    16201 ttaataagga ttttttccgc gcggaaaccg tcacggcggt gaatcagatg cacttccgaa
    16261 gcgatgttag acagatacag cgcctcttca accgcggtat tgccgccgcc gatgaccgca
    16321 actttctggt tgcgatagaa gaaaccgtcg caggttgcac aagcagaaac cccacggcct
    16381 ttaaaggctt cttcagaggg caggccgaga tagcgtgcag aagctccggt ggcaataatc
    16441 agcgcgtcgc aagtgtattc gccgttatcg ccattcagac ggaacggacg gttttgcaga
    16501 tccaccttgt tgatatgatc aaaaatgatc tcagtttcaa acttggtggc atgttcgtgc
    16561 atgcgctcca ttaataacgg accggtcaga tcgtttggat cgccaggcca gttttccact
    16621 tccgtggtgg tggtcagttg gccgcctttt tccatgccgg taatcagcac aggttgcagg
    16681 ttggcgcgcg ccgcgtagac agcagcggtg tatcccgccg ggcctgaacc caggataagc
    16741 agtttactgt gtttggtcgt gcccatgaga tccccatagt tgttggcaga caatgggcag
    16801 gattgtaggg aatttacaga cgtaaaaaaa gagtatgacg attttgttaa caatttgtgc
    16861 aatcggcagc atcgataagc aggtcaaatt ctcccgtcat tatcacctct gctacttaaa
    16921 tttcccgctt tataagccga ttaaatgatg aataaacgcc cctgttaatg aatatctggc
    16981 atgttgtact aaaaatcgat gttttgcttt gacaatcccc tggtgttttg cgaaaacatt
    17041 cgaggaagaa aaaaaacagt attcttatat gcgcataacc atgcatgtaa ataccatgtt
    17101 taccgtgcta gtgaaatcta cgtatggcgt ggacagacgc cattcgtgat gtcgatagct
    17161 gccacaaggc aacggtcttc tcaccgtaga cccaggcatt gcgcgccgtg aatcttcatg
    17221 atttcggtct atcgtgacgg gtagcgactc tgaacagtga tgtttcaggg tcagacagga
    17281 gtagggaagg aatacagaga gacaataata atggtagata gcaagaagcg ccctggcaaa
    17341 gatctcgacc gtatcgatcg taacattctt aatgagttgc aaaaggatgg gcgtatttct
    17401 aacgtcgagc tttctaaacg tgtgggactt tccccaacgc cgtgccttga gcgtgtgcgt
    17461 cggctggaaa gacaagggtt tattcagggc tatacggcgc tgcttaaccc ccattatctg
    17521 gatgcatcac ttctggtatt cgttgagatt actctgaatc gtggcgcacc ggatgtgttt
    17581 gaacaattca ataccgctgt acaaaaactt gaagaaattc aggagtgtca tttagtatct
    17641 ggtgatttcg actacctgtt gaaaacacgc gtgccggata tgtcagctta ccgtaagttg
    17701 ctgggggaaa ccctgctgcg tctgcctggc gtcaatgaca cacggacata cgttgttatg
    17761 gaagaagtca agcagagtaa tcgtctggtt attaagacgc gctaacacgg aacaggtgca
    17821 aaatcggcgt attttgatta cactcctgtt aatccataca gcaacagtac tggggtaacc
    17881 tggtactgtt gtccgtttta gcatcgggca ggaaaagcct gtaacctgga gagcctttct
    17941 tgagccagga atacactgaa gacaaagaag tcacattgac aaagttaagt agcggccgtc
    18001 gccttctgga agcgttgctg atccttattg tcctgtttgc cgtctggttg atggctgcct
    18061 tactaagctt taacccttcg gaccccagct ggtcgcaaac ggcctggcat gaacctatcc
    18121 ataatttagg tgggatgccc ggtgcgtggc tggcagatac gctgttcttt atttttggcg
    18181 tgatggctta caccattccc gtcattattg tcggcggttg ttggtttgcc tggcgtcatc
    18241 agtccagtga cgaatacatt gattattttg ccgtttcgct acgcatcatt ggcgttttgg
    18301 cgctcatcct tacctcctgt ggtctggcgg caatcaacgc tgacgatatc tggtattttg
    18361 cctccggtgg cgtcattggc agcttactaa gcactacgct acaaccactg ctacacagta
    18421 gcgggggaac tattgcgctg ctctgcgttt gggctgcggg cctgacgctg ttcaccggtt
    18481 ggtcatgggt gaccattgct gaaaaactcg gcggctggat tttaaacatt ctcaccttcg
    18541 ctagtaatcg tacccgtcgc gatgatacct gggtcgatga agatgaatat gaagatgacg
    18601 aagagtatga agatgaaaac cacggcaaac agcatgaatc acgccgtgcc cgtattcttc
    18661 gcggcgcgct agcgcgtcgt aaacggttgg cggaaaaatt cattaatccg atggggcggc
    18721 aaacagacgc tgcgttgttc tccggtaagc ggatggatga tgacgaagag attacctaca
    18781 ctgcacgcgg tgtggctgcc gaccctgacg acgtcctatt ttcgggcaat cgtgcaacgc
    18841 agccagaata tgacgaatac gatccattat taaacggtgc gccaattacc gaacctgtcg
    18901 ctgtagcagc tgctgctacc acggcgacac aaagctgggc tgcgccggtt gaacctgtga
    18961 ctcagacgcc gcctgttgcc tctgttgatg ttccaccttc gcaacctaca gtagcctggc
    19021 agcctgtacc gggtccacaa acgggagagc cggttattgc tcctgcaccg gaaggttacc
    19081 cacagcagcc acaatatgcg cagcctgcag tgcaatataa tgagccgctg caacaaccag
    19141 tacagccaca gcagccgtat tatgcacctg cagctgaaca acctgcgcaa cagccgtatt
    19201 atgcccctgc gccagaacaa ccggtggcag gtaacgcctg gcaagccgaa gagcagcaat
    19261 ccacttttgc tccacagtct acataccaga ctgagcaaac ttatcagcag ccagccgctc
    19321 aggagccgtt gtaccaacag ccgcaacccg ttgaacagca gcctgttgtg gagcctgaac
    19381 ccgttgtaga agagacaaaa cccgcgcgtc cgccgcttta ctattttgaa gaagtggaag
    19441 agaagcgagc ccgtgaacgt gaacaacttg cggcctggta tcaaccgatt ccagaaccgg
    19501 ttaaagaacc agaaccgatc aaatcttcgt tgaaagcgcc ttctgttgca gcagtacctc
    19561 cagtagaagc cgctgccgct gtttctccgc tggcatctgg cgtgaaaaaa gcgacactgg
    19621 cgacgggggc tgccgcaacc gttgccgcgc cagtcttcag tctggcaaat agcggtggac
    19681 cgcgtccgca agtcaaagag gggattggcc cgcagttgcc acgaccgaaa cgtatccgcg
    19741 tgccaactcg tcgtgaactg gcgtcttacg gtattaagct gccctcacag cgtgcggcgg
    19801 aagagaaagc ccgtgaagcc cagcgcaatc agtatgattc tggcgatcag tataacgatg
    19861 atgaaatcga tgcgatgcag caggatgaac tggcacgtca gttcgcccag acacagcagc
    19921 aacgctatgg cgaacagtat caacatgatg tgcccgtaaa cgcagaagat gcagatgctg
    19981 cggcagaggc tgaactggct cgtcagtttg cgcaaactca acaacaacgt tattccggcg
    20041 aacaaccggc tggggcgaat ccgttctcgc tggatgattt tgaattttcg ccaatgaaag
    20101 cgttgctgga tgatggtcca cacgaaccgt tgtttacgcc aattgttgaa cctgtacagc
    20161 agccgcaaca accggttgca ccgcagcagc agtatcagca gccacaacag ccggttgcgc
    20221 cacagcagca atatcagcag ccgcaacagc cggttgcgcc gcagccgcag tatcagcagc
    20281 cacaacagca ggttgcgccg cagccacaat atcagcagcc gcaacaaccg gttgcgccac
    20341 agccgcaata tcagcagccg caacaaccag ttgcgcagca gcagcaggat accctgcttc
    20401 atccgctgtt gatgcgtaat ggcgacagcc gtccgttgca taaaccgacg acgccgctgc
    20461 cttctctgga tttgctgaca ccgccgccga gcgaagtgga gccggtagat acctttgcgc
    20521 ttgaacaaat ggctcgcctg gtggaagcgc gtctggctga tttccgtatt aaagccgatg
    20581 tcgtcaatta ctctccgggg ccggttatca ctcgctttga acttaacctg gctcctggcg
    20641 taaaagcggc gcgcatttct aacttgtcac gggaccttgc ccgttcactt tcgacggtgg
    20701 cggtgcgtgt cgttgaagtt attcctggca aaccctatgt aggtctggag ttaccaaata
    20761 aaaaacgaca aaccgtttat ctgcgcgaag ttttggataa cgccaaattc cgcgataatc
    20821 cgtcgccatt aaccgtggtg ctgggtaaag atatcgccgg tgagccggtg gttgccgatc
    20881 tggcgaaaat gccgcacttg ttggttgcgg ggactaccgg ttccggtaaa tctgtcggtg
    20941 tgaacgcgat gatcctgagc atgctttata aagcacagcc agaagatgtg cgtttcatca
    21001 tgatcgaccc gaaaatgctg gagctttcgg tttatgaagg cattccgcat ctgttaacgg
    21061 aagtcgttac tgatatgaaa gatgccgcca acgcgctgcg ctggtgtgtt aacgagatgg
    21121 agcgtcgtta taaactgatg tctgcgctgg gtgtgcgtaa tctggcgggt tataacgaaa
    21181 aaattgctga agccgatcgc atgatgcgtc cgattccaga cccgtactgg aagccgggtg
    21241 acagtatgga tgcccagcat ccggtgctga aaaaagaacc atacattgtg gtgttggttg
    21301 acgaatttgc cgacctgatg atgacggtag gtaaaaaagt ggaagagctg atagcacgtc
    21361 tggcgcaaaa agcccgtgcc gcgggtatcc acctcgtact ggcaactcag cgtccatcgg
    21421 ttgatgttat tactggtctg attaaagcga atattccgac ccgtatcgcc tttaccgtat
    21481 ccagtaagat tgactcacgt accattcttg atcaggctgg cgcggaatca ctgctgggga
    21541 tgggggatat gctctactct gggccgaact ccacgttgcc ggtacgtgtc catggtgctt
    21601 ttgttcgcga tcaggaagtt catgccgtgg tgcaggactg gaaagcgcgt ggtcgcccac
    21661 agtatgttga tggcatcact tccgacagtg aaagcgaagg tggcgcgggc ggtttcgatg
    21721 gcgctgaaga actggatccg ttgttcgatc aggcggtgca gtttgtcact gaaaaacgca
    21781 aagcgtcaat ttctggcgta cagcgtcagt tccgcattgg ttataaccgt gcagcgcgta
    21841 ttatcgaaca gatggaagcg caggggattg tcagcgaaca ggggcacaac ggtaatcgtg
    21901 aagtgctggc cccaccgccg tttgactaat taatgcattg ccggataagg cgcggtagcg
    21961 tcgcatccgg cactctatca actgaaaatt cagtattttc ttctttcctc aagctgatta
    22021 ttagcctgga atagagagta gagggaactc ccgatcggga gtgacgtaat ttgaggaata
    22081 atgatgaaaa aaattgccat cacctgtgca ttactctcaa gcttagtagc aagcagcgtt
    22141 tgggctgatg ccgcaagcga tctgaaaagc cgcctggata aagtcagcag cttccacgcc
    22201 agcttcacac aaaaagtgac tgacggtagc ggcgcggcgg tgcaggaagg tcagggcgat
    22261 ctgtgggtga aacgtccaaa cttattcaac tggcatatga cacaacctga tgaaagcatt
    22321 ctggtttctg acggtaaaac actgtggttc tataacccgt tcgttgagca agctacggca
    22381 acctggctga aagatgccac cggtaatacg ccgtttatgc tgattgcccg caaccagtcc
    22441 agcgactggc agcagtacaa tatcaaacag aatggcgatg actttgtcct gacgccgaaa
    22501 gccagcaatg gcaatctgaa gcagttcacc attaacgtgg gacgtgatgg cacaatccat
    22561 cagtttagcg cggtggagca ggacgatcag cgcagcagtt atcaactgaa atcccagcaa
    22621 aatggggctg tggatgcagc gaaatttacc ttcaccccgc cgcaaggcgt cacggtagat
    22681 gatcaacgta agtagaggca cctgagtgag caatctgtcg ctcgattttt cggataatac
    22741 ttttcaacct ctggccgcgc gtatgcggcc agaaaattta gcacagtata tcggccagca
    22801 acatttgctg gctgcgggga agccgttgcc gcgcgctatc gaagccgggc atttacattc
    22861 tatgatcctc tgggggccac cgggtaccgg caagacaacc ctcgctgaag tgattgcccg
    22921 ctatgcgaac gctgatgtgg aacgtatttc tgccgtcacc tctggcgtga aagagattcg
    22981 cgaggcgatc gagcgcgccc ggcaaaaccg caatgcaggt cgccgcacta ttctttttgt
    23041 tgacgaagtg caccgattca acaaaagcca gcaggatgca tttctgccac atattgaaga
    23101 cggcaccatc acttttattg gcgcaaccac tgaaaacccg tcgtttgagc ttaattcggc
    23161 actgctttcc cgtgcccgtg tctatctgtt gaaatccctg ggtacagagg atattgagca
    23221 agtactaact caggcgatgg aagacaagac ccgtggttat ggtggtcagg atattgttct
    23281 gccagatgaa acacgacgcg ccattgctga actggtgaat ggcgacgcgc gccgggcgtt
    23341 aaatacgctg gaaatgatgg cggatatggc cgaagtcaat gatagcggta agcgggtcct
    23401 gaagcctgaa ttattgaccg aaatcgccgg tgagcgaagc gcccgctttg ataacaaagg
    23461 cgatcgcttt tacgatctca tttccgcact gcataagtcg gtacgtggta gcgcacccga
    23521 tgcggcgctc tactggtatg cgcgaattat taccgctggt ggcgatccgt tatatgtcgc
    23581 gcgtcgttgc ctggcaattg cttccgaaga tgtcggcaat gccgacccac gggcgatgca
    23641 ggtggcaatt gcggcctggg attgctttac tcgcgttggc ccggcggaag gtgaacgcgc
    23701 cattgctcag gcgattgttt acctggcctg cgcgccaaaa agcaacgctg tctacactgc
    23761 gtttaaagct gcgctggcgg atgctcgcga acgtccggat tatgacgtgc cggttcattt
    23821 gcgtaatgcg ccgacgaaat taatgaagga aatgggctac gggcaggaat atcgttacgc
    23881 tcatgatgaa gcaaacgctt atgctgccgg tgaggtttac ttcccgccgg aaatagcaca
    23941 aacacgctat tatttcccga caaacagggg ccttgaaggc aagattggcg aaaagctcgc
    24001 ctggctggct gaacaggatc aaaatagccc cataaaacgc taccgttaat gttatcgttg
    24061 cggtaatgtt gttactgtat ccctgtggtc gcaggctgtg gccacatctc ccatttaatt
    24121 cgataagcac aggataagca tgctcgatcc caatctgctg cgtaatgagc cagacgcagt
    24181 cgctgaaaaa ctggcacgcc ggggctttaa gctggatgta gataagctgg gcgctcttga
    24241 agagcgtcgt aaagtattgc aggtcaaaac ggaaaacctg caagcggagc gtaactcccg
    24301 atcgaaatcc attggccagg cgaaagcgcg cggggaagat atcgagcctt tacgtctgga
    24361 agtgaacaaa ctgggcgaag agctggatgc agcaaaagcc gagctggatg ctttacaggc
    24421 tgaaattcgc gatatcgcgc tgaccatccc taacctgcct gcagatgaag tgccggtagg
    24481 taaagacgaa aatgacaacg ttgaagtcag ccgctggggt accccgcgtg agtttgactt
    24541 tgaagttcgt gaccacgtga cgctgggtga aatgcactct ggcctcgact tggcagctgc
    24601 agttaagctg actggttccc gctttgtggt aatgaaaggg cagattgctc gcatgcaccg
    24661 cgcactgtcg cagtttatgc tggatctgca taccgaacag catggctaca gtgagaacta
    24721 tgttccgtac ctggttaacc aggacacgct gtacggtacg ggtcagctgc cgaaatttgc
    24781 tggcgatctg ttccatactc gtccgctgga agaagaagca gataccagta actatgcgct
    24841 gatcccaacg gcagaagttc cgctgactaa cctggtgcgc ggtgaaatca tcgatgaaga
    24901 tgatctgcca attaagatga ccgcccacac cccatgcttc cgttctgaag ccggttcata
    24961 tggtcgtgac acccgtggtc tgatccgtat gcaccagttc gacaaagttg aaatggtgca
    25021 gatcgtgcgc ccagaagact caatggcggc gctggaagag atgactggtc atgcagaaaa
    25081 agtcctgcag ttgctgggcc tgccgtaccg taaaatcatc ctttgcactg gcgacatggg
    25141 ctttggcgct tgcaaaactt acgacctgga agtatggatc ccggcacaga acacctaccg
    25201 tgagatctct tcctgctcca acgtttggga tttccaggca cgtcgtatgc aggcacgttg
    25261 ccgcagcaag tcggacaaga aaacccgtct ggttcatacc ctgaacggtt ctggtctggc
    25321 tgttggtcgt acgctggttg cagtaatgga aaactatcag caggctgatg gtcgtattga
    25381 agtaccagaa gttctgcgtc cgtatatgaa cggactggaa tatattggct aatacccaat
    25441 ttttctgaat ctaaaaagcg cctgcgggcg ctttttttgt ctccctttga taccgaacaa
    25501 taattactcc tcacttacac gtaatactac tttcgagtga aaatctacct atctctttga
    25561 ttttcaaatt attcgatgta tacaagccta tatagcgagc tgctatagaa ataattacac
    25621 aatacggttt gttactggaa tcaatcgtga gcaagcttga gtgagccatt atgaaaacga
    25681 aaatccctga tgcggtattg gctgctgagg tgagtcgccg tggtttggta aaaacgacag
    25741 cgatcggcgg cctggcaatg gccagcagcg cattaacatt accttttagt cggattgcgc
    25801 acgctgtcga tagcgccatt ccaacaaaat cagacgaaaa ggttatctgg agcgcctgta
    25861 cagttaactg tggtagtcgc tgcccgctac gtatgcacgt cgtggacggt gaaatcaaat
    25921 atgtcgaaac ggacaatacc ggcgatgaca attacgacgg cctgcaccag gttcgcgcct
    25981 gtctgcgtgg acgttccatg cgtcgccgtg tctataatcc ggaccgcctg aaatatccga
    26041 tgaaacgagt cggggcgcgc ggtgaaggca aatttgagcg cattagctgg gaagaagcct
    26101 acgacatcat cgcgaccaat atgcagcgcc tgatcaaaga gtacggcaac gaatccatct
    26161 atctgaacta tggcaccggt acgctgggcg gcaccatgac ccgctcctgg cccccgggaa
    26221 ataccctggt cgcgcgcctg atgaactgct gcggcggtta tctgaaccat tacggtgact
    26281 actcttccgc gcaaattgcg gaaggtttga actataccta cggcggctgg gcagatggta
    26341 acagcccgtc ggatatcgaa aacagtaaac tggtagtgct gtttggtaat aaccctggcg
    26401 aaacgcgaat gagtggcggt ggggtgactt actatcttga acaggcacgc cagaaatcta
    26461 atgcccgcat gatcatcatc gatccgcgct ataccgacac cggtgccggg cgcgaagatg
    26521 agtggatccc tattcgtccg ggaacagatg ccgcactggt taacggtctg gcgtacgtca
    26581 tgatcactga aaacctggtg gatcaggcat tcctcgataa atattgcgtt ggctacgatg
    26641 agaaaaccct gccagccagt gcgccgaaaa atggccacta taaagcttat attctgggtg
    26701 aagggccaga tggcgtggct aaaacaccgg aatgggcctc acaaatcacc ggtgttccgg
    26761 cagacaaaat catcaaactg gctcgtgaaa tcggcagtac caaaccggcg tttatcagcc
    26821 agggatgggg cccgcagcgt cacgctaacg gtgaaatcgc aacccgtgct atctcgatgc
    26881 tggcgattct gaccggtaac gttggtatta acggaggcaa cagcggcgcg cgtgaaggtt
    26941 catacagctt accgtttgtc cgtatgccga ccttggaaaa cccgatccag accagcattt
    27001 cgatgtttat gtggaccgat gccattgaac gtggcccgga aatgacggcg ctgcgtgatg
    27061 gtgtacgcgg gaaagataag ctggatgtgc cgatcaaaat gatctggaac tatgccggta
    27121 actgcctgat taaccagcat tctgaaatca accgtaccca tgaaatcctt caggatgata
    27181 agaagtgcga gctgattgtg gttatcgact gccacatgac ctcatcggcg aaatatgctg
    27241 acatcctgct gcctgactgc accgcttccg aacagatgga ctttgcgctg gatgcatcct
    27301 gcgggaatat gtcttacgtg attttcaacg atcaggtgat taaaccgcgc tttgaatgta
    27361 agaccatcta tgaaatgacc agcgaactgg caaaacgtct tggcgttgag caacagttta
    27421 ctgaaggccg tacccaggaa gagtggatgc ggcatctgta tgcccagtcg cgggaagcga
    27481 ttcctgaact gccaacgttt gaagagttcc gcaagcaggg gatctttaaa aagcgcgacc
    27541 cacaagggca tcacgttgct tataaagcct tccgtgaaga tccgcaggca aacccactga
    27601 ctacgccatc gggcaaaatt gagatttatt cgcaggcgct ggctgacatt gccgctacct
    27661 gggaattgcc agaaggcgat gtgatcgatc cactgccgat ctacacgccg ggctttgaaa
    27721 gttatcagga tccgttgaac aaacagtatc cgctgcagct tacgggtttc cactataaat
    27781 ctcgcgttca ctcaacttac ggcaacgttg atgtgctgaa agcagcttgc cgtcaggaaa
    27841 tgtggatcaa cccgcttgat gcgcaaaaac gcggtatcaa caacggcgat aaagtcagga
    27901 tctttaacga tcgtggtgag gttcatattg aggcgaaagt gacgccacga atgatgccgg
    27961 gtgtggtcgc actgggtgaa ggtgcctggt atgacccgga tacaaaacgt gtcgataagg
    28021 gcggttgtat caacgtactg accactcaac gtccgtctcc tctcgctaag gggaatccgt
    28081 cacatacaaa ccttgttcag gttgaaaagg tgtaaggagt aaccgatgac aacccagtat
    28141 ggatttttta ttgattccag ccgttgcacc ggttgcaaaa cctgcgagct ggcctgtaaa
    28201 gactacaaag atttgacgcc agaagtcagc ttccgccgca tttatgaata tgctggcggc
    28261 gactggcagg aagataacgg tgtctggcac cagaacgtgt ttgcctacta tctgtcgatt
    28321 tcatgtaacc actgcgaaga tccggcttgt actaaagtct gcccgagcgg tgcgatgcat
    28381 aaacgtgaag atggttttgt tgtggtcgat gaagatgtgt gcattggctg ccgctactgc
    28441 catatggctt gcccgtatgg cgcaccgcaa tataacgaaa cgaaaggcca tatgaccaaa
    28501 tgcgatggtt gttatgaccg tgttgcggag gggaaaaagc cgatctgtgt tgaatcttgc
    28561 ccgctgcggg cgctggattt cggccctatc gacgagctgc gtaaaaaaca tggcgatctg
    28621 gcggccgttg cgccgttgcc gcgagctcac tttaccaaac cgaatattgt gatcaaaccc
    28681 aatgccaata gccgcccgac cggggatacc actggctatc tggcaaaccc gaaggaggtg
    28741 tgagatggga agtggatggc atgaatggcc gctgatgatc ttcacggtct tcgggcaatg
    28801 tgtagcaggt ggttttatcg ttctggcttt ggcgctgctc aaaggcgacc tgcgagcaga
    28861 agcccagcag cgtgttatcg cctgcatgtt tggtttatgg gtgctgatgg gcattggctt
    28921 tatcgcctct atgctccatc ttggttcacc aatgcgcgct tttaactcgc tcaaccgggt
    28981 aggggcttca gcactcagta acgaaatcgc cagcggttcg atcttttttg ccgtaggcgg
    29041 catcggctgg ttgctggcaa tgctgaaaaa gctgtcaccg gcattgcgta cgctgtggct
    29101 gatagtgacg atggttcttg gcgtcatctt tgtctggatg atggtgcgtg tgtataacag
    29161 cattgatacc gttccgacct ggtacagcat ctggacgccg atgggcttct tcctgacgat
    29221 gtttatgggc ggcccgttac tgggttacct gttgttgagt ctggctggcg tcgatggctg
    29281 ggcgatgcgt ctgctgccag caatttctgt actggcactg gtagtgagtg gcgtggtgtc
    29341 agtgatgcag ggcgcagagc tggcgactat tcatagttct gtgcagcagg ccgcagcgct
    29401 ggtgccggac tatggtgcgc tgatgtcctg gcggatcgtg cttttggccg ttgccctgtg
    29461 cttgtggatt gcaccacagc taaaaggtta tcagcctgcg gttccgctac tttctgtatc
    29521 attcattctg cttctggcag gggaattaat cggtcgcggc gtattctatg gtttgcatat
    29581 gaccgtgggg atggccgtcg caagctaatc ataacaaccg gggtttcggc cccggttctc
    29641 ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata atcaggaatg
    29701 tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcgcg atgcagctcg
    29761 cacgccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg atcccatgcc
    29821 gaatgccggg taatttcatt aaaggtccct gacgcgtcag tcacgacaaa gacgtcaaac
    29881 ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac cacaccggca
    29941 ataattaact gttttttacc tgtcgctttg acagctttta caaaatcttc gttatcccag
    30001 gcgttaatat ttcccgggcg agcaatgtaa ggtgtatcgg ggaattgtgc tttcagttct
    30061 ggaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt tggtaagttg
    30121 aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc gggttcgata
    30181 tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc agcatcattt
    30241 ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc agtggtacgt
    30301 ctgaaatcga tcagacgcga tgcattgctc tgaaagcata gacgggaaat atgagtttgc
    30361 tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag ttcaataaaa
    30421 acaatcagat gagactaatc atctcttcag cattagcgtt atgaatgagt aatataaatc
    30481 gataataccg ccgctgggat atcgcgtatt ttcacccatt gacaatgttt ttggcggtgg
    30541 catgatgcgc atgaaatttg aacttcctca cggttttaat tcatgtccac gtatacccgg
    30601 cctgtcatgc ttttgctgtc tggcctgctt ttgttgactc tggcgattgc ggtgttaaat
    30661 acactcgtgc cgctttggct cgcccaggaa cacatgtcca catggcaggt aggcgttgtc
    30721 agctcatcct attttaccgg caaccttgtc ggtacattgc tgacagggta tgtcattaag
    30781 cgcattggct ttaaccgcag ctattatctg gcctccttca tttttgccgc tggctgtgcc
    30841 ggccttggcc tgatgattgg attctggagc tggttggctt ggcgttttgt cgcgggcgtc
    30901 ggctgtgcca tgatttgggt ggttgttgag agcgcgctga tgtgcagtgg gacgtcacgt
    30961 aaccgtgggc gtttgcttgc tgcgtatatg atggtttatt acgtgggaac gtttttaggc
    31021 cagttactgg tcagcaaagt ttcaaccgag ctgatgtccg tattgccgtg ggttacaggt
    31081 ttgacgttgg cagggatctt accgctgttg tttacgcgtg tgctgaatca gcaggctgaa
    31141 aaccatgatt cgacgtcaat tacgtcaatg ctaaaactcc gtcaggcgcg gcttggcgtg
    31201 aatggctgca ttatctcagg aatcgttctg ggatctctat atggcctgat gccgctgtac
    31261 ctcaatcaca aaggggtgag caatgccagc attggtttct ggatggcggt actggtcagt
    31321 gcgggtatcc ttggacaatg gccgattgga cgtctggcgg ataagtttgg tcgactgctg
    31381 gtgttgcgtg ttcaggtctt tgtcgtcatt ctcggcagta tcgcgatgct tagccaggcg
    31441 gcgatggccc cagcgttatt catcctcggt gccgctggct ttacgctata tccggtggcg
    31501 atggcatggg cttgcgagaa agttgaacat catcaactgg tggcgatgaa ccaggcctta
    31561 ctgttgagct atactgtggg aagtctgctt ggcccgtcat ttaccgctat gctaatgcag
    31621 aatttctccg ataatttatt gtttatcatg atcgccagcg tatcgtttat ctatttgctg
    31681 atgctgctgc gcaacgccgg tcatacgccg aaacccgttg ctcacgtgta aatgaattca
    31741 agcagagtgt gaacttactg tttcacactc tgcttttttg tttcttctat ctgacttgct
    31801 ttattccaaa ttttattcgt ttaaaaataa aatgtgcagc aggttataat tttgcatttc
    31861 gctatttccg cacttcttat ttgccgcgca taatccctcg ttttacagat gcccctttaa
    31921 ttttggcgaa ggatttgtct atggctggga atgttcagga aaaacagttg cgatggtaca
    31981 acattgcgct gatgtctttt atcactgtct ggggttttgg caacgttgtt aataactatg
    32041 ccaaccaggg gctggtggtt gttttttcat gggtgtttat ctttgcactc tatttcacac
    32101 cttatgcgct aattgttggt cagttaggct cgaccttcaa agatgggaag ggcggggtca
    32161 gtacctggat taaacacacg atgggacccg gactggctta tctcgccgcg tggacctact
    32221 gggtggtgca tattccctat ctggcacaaa aaccccaggc aattctgatt gcgctcggtt
    32281 gggcgatgaa aggcgacggt tcgctaatca aagaatattc agtcgtagcg ttacaggggt
    32341 taacgctggt gctgtttatc ttctttatgt gggttgcttc acgcggtatg aaatcgctga
    32401 aaatcgtcgg ttctgtggca gggattgcga tgtttgttat gtcgctcctg tatgtggcga
    32461 tggcggtaac cgcgcctgcg attactgaag tgcatattgc gaccacaaac attacctggg
    32521 aaacgttcat tcctcatatc gactttacct acattaccac tatttcaatg ctggttttcg
    32581 cggttggcgg agcagagaag atttctcctt acgttaatca aacgcgcaac ccaggaaaag
    32641 aatttccaaa agggatgtta tgcctggcgg tgatggttgc ggtttgtgcc attctgggct
    32701 cactggcgat ggggatgatg tttgactcgc gtaatatccc ggatgactta atgactaacg
    32761 gtcaatatta cgcctttcag aagctgggcg agtattacaa catgggtaat actttaatgg
    32821 tgatttacgc cattgcgaat accctgggac aagtagctgc gctggtattc tcgattgatg
    32881 ccccgcttaa agtgctatta ggtgatgctg acagcaaata tattccagcc agtttatgtc
    32941 gtaccaacgc ttctggtacg cccgttaatg gctattttct gaccctggtg ctggtggcaa
    33001 ttctgattat gctgccgact ctcggcatcg gtgatatgaa caatctctat aaatggctgt
    33061 tgaatcttaa ctcggtagtg atgccgctgc gttatttatg ggtatttgtt gcatttattg
    33121 cagtcgttcg cctggcgcag aaatataaac cagaatatgt ctttattcgt aataagcctc
    33181 tggcaatgac cgtcgggatt tgtgtttttc ctttaccgcc tttgcctgct tgacggggat
    33241 cttcccgaaa atggaagcct tcactgcaga gtggaccttc cagttggcgc tgaatgttgc
    33301 aacgccgttt gtgctggtag gattaggact gatattcccg ctgctggcgc gtaaagcgaa
    33361 tagtaaataa ttattgtggt cggtgacgct gactctggca gatctgaccc gtttttacat
    33421 aatcgagaaa tgcgcgcaag ccgcaggaca tatactggcg gtttggataa tagatctgga
    33481 agcctgaacg ctctgtgctc cagtcttcca gtacacattc taaacgacca gtatcaaggt
    33541 actccttgat ctgttcatag agcagatacc cgataccagc acccatcaaa acggcctcca
    33601 gttcagcatc aacatcatcc agaatgatat ttcctgcgac agcaatttcc agctcctttg
    33661 caaattgcca gtggaaaggt ttgccgctgg gataacgaaa aacaacacat tgatgattaa
    33721 gcagatcatg aggatggcgg ggtttgccat atcttgcaaa gtactctggc gttgctgcta
    33781 cacataattt cactggtggg ccgatcgcca cgctgatcat atctttttcg acgatgccac
    33841 taagtcgaac gccagcgtca aagccctgtt ggacgatatc gacaagagaa tcgtcagttg
    33901 tgagctcgac tttaatatca ggatattcac gtgtgaaacc aaccagtaaa ggcattaaaa
    33961 atatacgtgc agctacccgg gcggcattga ttttgagcgt tcctgtcggt gtcagacgaa
    34021 aatcattcat ctcatctaac atgatctgaa tttcgtcgaa agctgggcgc aatcgttcat
    34081 agagattaga acccgcttcc gttaacgata cgcttcgggt tgtccgattg aataagcgaa
    34141 ttttaagacg ttgttccagt gttttaatac tatggctaat ggcggacgag gataagccta
    34201 actcatcgcc cgctgcacga aagctttgat tacgggcgac ggcaaagaaa gtggcaaagt
    34261 cagacatatt catccgcatt agtgaaaatc cttcattaac tcatcctgaa tcatcacgtt
    34321 tacaggatta tgccgcctgc gtaaagtgcg ctccagaact taacgtggag gtaaaattat
    34381 gcagtctgaa cgtatttatt tggtatgggc ccatcctcgt catgattcat tgaccgcaaa
    34441 tattgctgat gcgatccatc agcgggcaat ggagcggaaa atacaggtga cggaactcga
    34501 tttatatcgg cgtaatttca acccagtgat gacgccggaa gatgaaccag actggaagaa
    34561 tatggataaa cgttattctc cagaggttca tcagctttat tcagagctgc ttgaacatga
    34621 cacgttagtg gtggtttttc ctctctggtg gtacagcttc ccggcaatgc taaaaggata
    34681 tattgacaga gtatggaata atgggctggc ttatggagat gggcacaaat taccattcaa
    34741 taaagttcgt tgggtggcgc tggttggagg agacaaagaa tcatttgtcc agatgggctg
    34801 ggaaaaaaat ataagcgatt atttgaaaaa tatgtgcagt tatcttggta ttgaagatgc
    34861 cgatgtcact ttcttgtgta atacagtggt attcgatggg caagaacttc atgcgagcta
    34921 ttatcagtcg ttattatctc aggtacggga tatggtagat gcactataaa atgtgttaaa
    34981 aacgctgtag cagaatgaag cgcggaataa aaaagcggca actcaataaa gttgccgctt
    35041 tacggggaaa ttagaacatt accttatgac cgtactgctc aagaatgcct ttcacgcgtt
    35101 ccatggtctc tttcttaggt ggtttaacac cgtcgagttt gtactcttca cccattgcca
    35161 cccatttgtg tttgcccagc tcgtggtagg ggaggagttc gattttctca acgttgccca
    35221 tatcacgggt aaattcaccg aggcgatgcg ctgaatcgtc atcgtcagac cagccaggga
    35281 caacaacgta gcggatccac accttcacat ttttgttcgc cagatatttg gcgaactcca
    35341 gcgtgcggtg gttggaaact ccaaccagat tttggtggat ctcgtcgttc atctgtttga
    35401 gatcgagcat taccaggtcg gttacttcca gcagttcatc aatcaccgga tcgtaacgac
    35461 gaacaaaacc gttggtgtcc agacaggtat gaatgccttc ttttttgcag gcgcggaacc
    35521 agtcacgaac aaattcagct tgtaggattg cctcaccgcc ggatgcggta acgccgccgc
    35581 cggaagcgtt cataaagtgg cgataggtca ccacctcttt cattaaatct tcaacggtaa
    35641 cttctttacc gccatgcgta tcccaggtgt cgcggttatg acaatacagg cagcgcatca
    35701 ggcagccctg gaaaaaggtg ataaagcgaa tacctgggcc gtctacggtt ccacaggatt
    35761 caaaggagtg aatgcgacca ataactgaca ttgcggtgtt tctccagatg tggcccatct
    35821 gaggccgtgt tggtgcgcag ctcgaaggct acgtcgagtc tgttttggca gtcaccttaa
    35881 agtatagata gctgacaaaa aaggctctcg tgctaaaaaa ggccccactt tcgtggagcc
    35941 tttattgtac gctttttact gtacgatttc agtcaaatct aattacatag attgagtgaa
    36001 ggtacgagta ataacgtcct gctgctgttc tttagtcagc gagttgaaac gtactgcgta
    36061 gccagataca cggatggtca gctgcggata tttttccggg ttttccatcg cgtcgagcag
    36121 catttcacgg ttcatcacgt taacgttcag gtgctgacca ccttcgatgg atgcttcgtg
    36181 gtggaagtaa ccatccatca gaccagccag gttggtctta cgaacttcgt cgtctttacc
    36241 cagtgcgttc ggaacgatag agaaggtgta ggagatacca tctttagcgt aagcaaacgg
    36301 cagtttagca acggaagtca gagacgctac agcacctttc tggtcacgac cgtgcatcgg
    36361 gttagcaccc ggtccgaacg gcgcgccagc acgacgaccg tctggggtgt taccagtttt
    36421 cttaccatac acaacgttag aagtgatggt cagaacagac tgagtcggga tagcgtcacg
    36481 gtaggtgtgc agtttctgaa ttttcttcat gaaacgttct accaggtcaa cagccaggtc
    36541 atctacacgc ggatcattgt taccaaactg cgggtattcg ccttcgattt cgaagtcgat
    36601 agccagaccg tcttcgtcac gaatcggttt aactttcgca tatttgattg cagacaggga
    36661 gtcagcagca acggacagac cagcgatacc acacgccatg gtgcggataa cgtcacggtc
    36721 gtgcagcgcc atcagagagg cttcgtagct gtacttgtcg tgcatgtagt ggatgatgtt
    36781 cagtgcagtg atgtactgtt tagccagcca gtccatgaag tgatccatgc gctccatcac
    36841 ttcatcatag ttcaggacat cgcctttgat cggttcagac ttcggaccaa cctgcatttt
    36901 cagtttttcg tcaacgccgc cgttgattgc gtacagcatg gttttcgcca ggtttgcacg
    36961 cgcaccgaag aactgcattt gtttaccaac gatcatcggg cttacgcagc aagcaatagc
    37021 gtagtcatcg ttgttgaagt ccggacgcat caggtcatcg ttctcatact gcagagaaga
    37081 ggtgtcgatg gacactttag cggcgaattt cttgaagttc agcggcagtt tttcagacca
    37141 cagaatggtc atgttcggtt ccggagacgg acccatggtg tacagggtgt tcaggaaacg
    37201 gaagctgttt ttggtaacca gggtacgacc gtcgaggccc ataccaccga tagattcggt
    37261 tgcccagatc gggtcgccag agaacagttc atcgtattcc ggagtacgca ggaagcgaac
    37321 catacgcagt ttcatgacca ggtggtcaac catttcctgc gcttcttgtt cggtgatctt
    37381 gccagctttc aggtcacgtt cgatgtacac atccaggaag gtggaggtac gaccgaagga
    37441 cattgcagca ccgttctgag acttaacagc agccaggtag ccgaagtaag tccactggat
    37501 agcttcctga gcgttggtag ccggaccaga gatgtcgtag ccgtatttcg cagccatttc
    37561 tttcatctga cccagagcgc ggtgctgttc agcgatttct tcgcgcagac ggatagtctg
    37621 ttccaggttt acgccgtttt ccagatcagc ctgcagagaa gtgaactgtg ccagtttgtc
    37681 tttcatcagg tagtcgatac cgtacagcgc aacgcgacgg tagtcaccga tgatacggcc
    37741 acggccatat gcatctggca gaccggtcag aacaccagat ttacggcaac gcaggatgtc
    37801 cggagtgtaa acgtcgaaca cgccctggtt gtgagtttta cggtattcag tgaagatttt
    37861 tttgatcatc ggatccagtt cgcggttgta cgctttgcag gaaccttcga tcattttgat
    37921 accaccgaac gggataagag cacgtttcag cggagcttca gtctgcagac caacgatttt
    37981 ctcaagctgc ttgttgatgt agccagcgtc gtgagaggtg atggtggaag caacagcggt
    38041 gtcaaagtca actggcgcgt gagtgcggtt ttccagttta acgccttcca ttactttgtc
    38101 ccacagggtg gtggtcgctt cagtagcgcc agccaggaag gactcgtcac cctcgtacgg
    38161 agtgtagttt ttctgaatga agtcacggac gtttacttca ttctgccagt cacctttggt
    38221 aaaaccttcc caggctgtgg ctaacttttc attaagctcg gacatgtaac acctaccttc
    38281 ttaagtggat tttttattta ctgcgtactt cgacaaccat taatggtggt cgttttcacg
    38341 caggtaaatg acccagtatg tcaacccaac caacaaacca ccaccgataa tgttgccgat
    38401 cgtaaccgga atcaggttat cagtgatgaa attcatcacg gtcaggtgag aaaaattttc
    38461 cggtgcagaa ccgactgcgg tccaaaattc cggggatgcg aagtcgcgga ttacaatacc
    38521 catcgggatc ataaacatgt ttgcgatact gtgctcaaaa ccgctggcaa caaacatcgc
    38581 gaccggcagc accataatga acgctttgtc catcaggctg cggccagaat aactcatcca
    38641 tactgccaga cataccatca ggtttgccag gataccaaga cagacggcct caataaaagt
    38701 atggtgcact ttgtggtcgg cggtttgtag gacgtttagt ccccattgac catttgcggt
    38761 catatactcg ccggaaagcc acattaaaag tacaaacagc agtgcgccga ccaggttgcc
    38821 aaaatagaca tttagccagt ttttcgccaa ctgaccccag gtgatgcgcc cactcgcctt
    38881 agcaacaaca atcaacacgg tggaagtaaa gagatcggct ccgcagacaa cacaaagaat
    38941 cagccccaga gagaagcaaa tgccgccaac cagttttgcc atgccgaagg gcattgtgcc
    39001 tgtgccagtg gttgctgtga tatagaagac gaatgcgatt gagatgaaaa caccggcggt
    39061 aatcgccaga tagaaagtct taagcggatg tttcgttgct ttatagacac ccgcctcttc
    39121 ggccactttg gccattgcag caggaagtaa aagatcaaaa gggttgtcag ctttcacact
    39181 aactctctct ttattaagtc ggcgacgaga tactaacaaa gcattataga tgagaaattg
    39241 atatagatca tatctcgcct ggctt
//
LOCUS       DAZKIV010000025        37584 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZKIV010000025 DAZKIV010000000
VERSION     DAZKIV010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37584
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.025"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            289..1662
                     /gene="aroP"
                     /locus_tag="ACHOT6_001351"
     CDS             289..1662
                     /gene="aroP"
                     /locus_tag="ACHOT6_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFP9946594.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(1705..2559)
                     /gene="ampE"
                     /locus_tag="ACHOT6_001352"
     CDS             complement(1705..2559)
                     /gene="ampE"
                     /locus_tag="ACHOT6_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFP9946595.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVNKVETPRAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(2556..3107)
                     /gene="ampD"
                     /locus_tag="ACHOT6_001353"
     CDS             complement(2556..3107)
                     /gene="ampD"
                     /locus_tag="ACHOT6_001353"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFP9946596.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            3195..4088
                     /gene="nadC"
                     /locus_tag="ACHOT6_001354"
     CDS             3195..4088
                     /gene="nadC"
                     /locus_tag="ACHOT6_001354"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFP9946597.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            4291..4731
                     /gene="ppdD"
                     /locus_tag="ACHOT6_001355"
     CDS             4291..4731
                     /gene="ppdD"
                     /locus_tag="ACHOT6_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFP9946598.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVTKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            4741..6114
                     /gene="gspE"
                     /locus_tag="ACHOT6_001356"
     CDS             4741..6114
                     /gene="gspE"
                     /locus_tag="ACHOT6_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFP9946599.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHIAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVELAGNA
                     VSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLVLVTG
                     PTGSGKTVTLYSALQKLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGVLRAL
                     LRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVARWML
                     SSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGFYGRT
                     ALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEELIRV
                     LGMPHGE"
     gene            6104..7306
                     /gene="hofC"
                     /locus_tag="ACHOT6_001357"
     CDS             6104..7306
                     /gene="hofC"
                     /locus_tag="ACHOT6_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFP9946600.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNTLLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTEKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(7341..8384)
                     /gene="guaC"
                     /locus_tag="ACHOT6_001358"
     CDS             complement(7341..8384)
                     /gene="guaC"
                     /locus_tag="ACHOT6_001358"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFP9946601.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(8540..8584)
                     /locus_tag="ACHOT6_001359"
     CDS             complement(8540..8584)
                     /locus_tag="ACHOT6_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946602.1"
                     /translation="MIIKTLKMSARKRN"
     gene            8609..9229
                     /gene="coaE"
                     /locus_tag="ACHOT6_001360"
     CDS             8609..9229
                     /gene="coaE"
                     /locus_tag="ACHOT6_001360"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFP9946603.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            9229..9972
                     /gene="zapD"
                     /locus_tag="ACHOT6_001361"
     CDS             9229..9972
                     /gene="zapD"
                     /locus_tag="ACHOT6_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFP9946604.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            9982..10179
                     /gene="yacG"
                     /locus_tag="ACHOT6_001362"
     CDS             9982..10179
                     /gene="yacG"
                     /locus_tag="ACHOT6_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFP9946605.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(10278..10667)
                     /gene="mutT"
                     /locus_tag="ACHOT6_001363"
     CDS             complement(10278..10667)
                     /gene="mutT"
                     /locus_tag="ACHOT6_001363"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFP9946606.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(10727..13432)
                     /gene="secA"
                     /locus_tag="ACHOT6_001364"
     CDS             complement(10727..13432)
                     /gene="secA"
                     /locus_tag="ACHOT6_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFP9946607.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(13494..14006)
                     /gene="secM"
                     /locus_tag="ACHOT6_001365"
     CDS             complement(13494..14006)
                     /gene="secM"
                     /locus_tag="ACHOT6_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFP9946608.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLS"
     gene            complement(14237..15154)
                     /gene="lpxC"
                     /locus_tag="ACHOT6_001366"
     CDS             complement(14237..15154)
                     /gene="lpxC"
                     /locus_tag="ACHOT6_001366"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFP9946609.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(15255..16406)
                     /gene="ftsZ"
                     /locus_tag="ACHOT6_001367"
     CDS             complement(15255..16406)
                     /gene="ftsZ"
                     /locus_tag="ACHOT6_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFP9946610.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(16467..17729)
                     /gene="ftsA"
                     /locus_tag="ACHOT6_001368"
     CDS             complement(16467..17729)
                     /gene="ftsA"
                     /locus_tag="ACHOT6_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFP9946611.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(17726..18556)
                     /gene="ftsQ"
                     /locus_tag="ACHOT6_001369"
     CDS             complement(17726..18556)
                     /gene="ftsQ"
                     /locus_tag="ACHOT6_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFP9946612.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPIL
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(18558..19478)
                     /gene="ddlB"
                     /locus_tag="ACHOT6_001370"
     CDS             complement(18558..19478)
                     /gene="ddlB"
                     /locus_tag="ACHOT6_001370"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9946613.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(19471..20946)
                     /gene="murC"
                     /locus_tag="ACHOT6_001371"
     CDS             complement(19471..20946)
                     /gene="murC"
                     /locus_tag="ACHOT6_001371"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFP9946614.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLESVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(21000..22067)
                     /gene="murG"
                     /locus_tag="ACHOT6_001372"
     CDS             complement(21000..22067)
                     /gene="murG"
                     /locus_tag="ACHOT6_001372"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFP9946615.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYTWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(22064..23308)
                     /gene="ftsW"
                     /locus_tag="ACHOT6_001373"
     CDS             complement(22064..23308)
                     /gene="ftsW"
                     /locus_tag="ACHOT6_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFP9946616.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(23308..24624)
                     /gene="murD"
                     /locus_tag="ACHOT6_001374"
     CDS             complement(23308..24624)
                     /gene="murD"
                     /locus_tag="ACHOT6_001374"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFP9946617.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(24627..25709)
                     /gene="mraY"
                     /locus_tag="ACHOT6_001375"
     CDS             complement(24627..25709)
                     /gene="mraY"
                     /locus_tag="ACHOT6_001375"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFP9946618.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLVGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(25703..27061)
                     /gene="murF"
                     /locus_tag="ACHOT6_001376"
     CDS             complement(25703..27061)
                     /gene="murF"
                     /locus_tag="ACHOT6_001376"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFP9946619.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(27058..28545)
                     /gene="murE"
                     /locus_tag="ACHOT6_001377"
     CDS             complement(27058..28545)
                     /gene="murE"
                     /locus_tag="ACHOT6_001377"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFP9946620.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(28532..30298)
                     /gene="ftsI"
                     /locus_tag="ACHOT6_001378"
     CDS             complement(28532..30298)
                     /gene="ftsI"
                     /locus_tag="ACHOT6_001378"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFP9946621.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFVLLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERIFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(30314..30679)
                     /gene="ftsL"
                     /locus_tag="ACHOT6_001379"
     CDS             complement(30314..30679)
                     /gene="ftsL"
                     /locus_tag="ACHOT6_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFP9946622.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(30676..31617)
                     /gene="rsmH"
                     /locus_tag="ACHOT6_001380"
     CDS             complement(30676..31617)
                     /gene="rsmH"
                     /locus_tag="ACHOT6_001380"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFP9946623.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(31619..32077)
                     /gene="mraZ"
                     /locus_tag="ACHOT6_001381"
     CDS             complement(31619..32077)
                     /gene="mraZ"
                     /locus_tag="ACHOT6_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFP9946624.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMICTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(32679..33683)
                     /gene="cra"
                     /locus_tag="ACHOT6_001382"
     CDS             complement(32679..33683)
                     /gene="cra"
                     /locus_tag="ACHOT6_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFP9946625.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(33765..33851)
                     /locus_tag="ACHOT6_001383"
     CDS             complement(33765..33851)
                     /locus_tag="ACHOT6_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFP9946626.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(33863..34354)
                     /gene="ilvN"
                     /locus_tag="ACHOT6_001384"
     CDS             complement(33863..34354)
                     /gene="ilvN"
                     /locus_tag="ACHOT6_001384"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9946627.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(34357..36081)
                     /gene="ilvI"
                     /locus_tag="ACHOT6_001385"
     CDS             complement(34357..36081)
                     /gene="ilvI"
                     /locus_tag="ACHOT6_001385"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFP9946628.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVCSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTNGDVYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            36200..36244
                     /locus_tag="ACHOT6_001386"
     CDS             36200..36244
                     /locus_tag="ACHOT6_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946629.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(36399..37343)
                     /gene="leuO"
                     /locus_tag="ACHOT6_001387"
     CDS             complement(36399..37343)
                     /gene="leuO"
                     /locus_tag="ACHOT6_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFP9946630.1"
                     /translation="MPEVQTDHPETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         9086 a        10454 c         9163 g         8881 t
ORIGIN      
        1 accaattttg tgatttttaa ttcaattaaa acgaatttaa attcattcta catattgaga
       61 ggggttgagg ctgagcttta caaacggttt ctttttaagc aactcatctt caaccatgca
      121 taaagcgggt gcattcgctg ccgcatacca ttattcttga tctgacggaa gtctttttgc
      181 aacaattcaa acttctttga tgtaaacaaa ttaatacaac aaacggaatt gtaaacttac
      241 acacgcatca ctgcgtagat caaaaaaaca accaccgcac gaggtttcat gatggaaggt
      301 caacagcacg gcgagcagct aaagcgcggc cttaaaaacc gccatattca gcttatcgcg
      361 ctgggtggcg cgatagggac cgggttattc ctgggtagcg cctccgtaat acagtccgca
      421 gggccaggga ttatcctggg ttacgccatt gctggtttta tcgcctttct gatcatgcgt
      481 cagctgggtg aaatggtggt cgaagaacct gtcgcaggct cctttagcca ctttgcttat
      541 aaatactggg gcagttttgc cggtttcgcc tctggctgga actactgggt actgtacgtt
      601 ttagttgcca tggctgagct gactgccgtg ggtaaataca ttcagttctg gtatccggaa
      661 atccccacct gggtttctgc cgccgtattc tttgtggtga ttaacgccat caacctgacc
      721 aacgttaaag tgtttggcga gatggagttc tggtttgcca ttatcaaagt tatcgcggtg
      781 gtagcgatga tcatcttcgg cggctggctg ctattcagtg gcaacggcgg cccgcaggcg
      841 agcgttagca acctgtggga tcagggtggt ttcctgccgc acggcttcac cgggctggtg
      901 atgatgatgg cgattatcat gttctcgttc ggtggtctgg aactggtggg gatcaccgca
      961 gcagaagctg ataacccgga gcaaagtata ccgaaagcaa ctaaccaggt tatctaccgc
     1021 atcctgattt tctatattgg ttcgttagcc gttctgctct cactgatgcc gtggacccgc
     1081 gttaccgccg ataccagtcc gtttgtgctg atcttccacg agttaggcga tacctttgtg
     1141 gcgaatgcgc tgaacatcgt ggtactgact gcggcgctct ccgtgtacaa cagctgcgta
     1201 tattgcaaca gccgtatgct gtttggtctg gcacaacagg gtaatgcgcc aaaagcgctg
     1261 gcgtctgtcg ataaacgtgg tgtaccagta aataccattc tggtgtctgc actggtaacg
     1321 gcgttgtgcg tactgattaa ctaccttgcc ccagagtccg ctttcggact gttaatggcg
     1381 ctggtggtat ctgcactggt aatcaactgg gcgatgatta gcctggcgca tatgaaattc
     1441 cgtcgcgcca agcaggaaca aggcgtggta actcgcttcc ctgctctgct ttatccgctg
     1501 ggtaactgga tctgcctgct gtttatggcg gcggtactgg tgattatgct gatgacccca
     1561 ggaatggcga tttcggtata cctgatcccg gtatggctga tcgtgttagg tatcggctat
     1621 ctgtttaaag agaaaaccgc caaagccgta aaagcgcatt aatctctcta cgccctcacc
     1681 cgtacagggt gagggcaata atctttacac caacgccccg taaatcgtca gtagtgcaat
     1741 caccaccacg accacgaacg aggttttctt cgccattgaa accgctgccc tcggcgtctc
     1801 caccttattg acatgcggtt cacgcgccag agagaactgc gccagacgcg ttaacacctg
     1861 atactgcgaa gtatggaaat cacccagcga agcaaaccag gccggtaacg ctttctcacc
     1921 atgaccgatc aaggcatata ccacacccgc aagacgaacc ggcacccaat ccagtacatg
     1981 aagcacggca tcaatgccgg actgtaaacg atgatgcggc gtctgatatc gcgccagcca
     2041 gtattgccat gcacgcaaaa acgcataccc catcagcgta acgggtcccc aggttccccc
     2101 cacaatcagc cagaacagcg gtgcaagata aaaacgaaag ttaatccaca gcaatgcatt
     2161 ttgcagctca cgcaaatact cacgttcgtc gcagcctgcc gggacgccgt gaatcatggt
     2221 gagttcgcca gccatcgtgg cacgggcatg gctatcatta cgtgaagcag ctgtcagata
     2281 agcatgataa tgaagacgaa ctttacctgc gccaatacac agcaaaccaa tcagcagcca
     2341 caccagtagc gtgggaacgt tgaacaatac tccctgcaat gcgcgtaaca gtaaaaaagt
     2401 cacgcccatc gcaataatgg tcatgcctaa cgtgcgcccg agagaaaaat gtttcacccg
     2461 ccgaaagaac gcttcaagac gatgatcaag ctgccagtgc tcgcccaact taaacaggcg
     2521 ctcgaaaatt aacaccagta aggttgtaaa tagcgtcatg ttgtctcctt gctgaccagc
     2581 acacgaaacc gtgcccaatc aaatgcagga ccgggatcgg ttttccgatc cggcgcaata
     2641 tcacaatggc ccgtcatgtt tttagcgata tccggatagc aatcaatcag tgcccgcgta
     2701 accgccgcaa gctgttgata ctgcgcatcg gtatacgcca gcgtatcggt gccttcaagc
     2761 tcaatcccaa tagaaaaatc attgcagcgt tcgcgcccct gatactgaga gactcccgca
     2821 tgccatgcac gtttatcgaa aggaacatac tggactattt caccatcacg gcgaatcaaa
     2881 cagtgagcgg agacgcgcaa atgggcgatc tcagcaaaga aaggatgtgc ctgcggatca
     2941 atagttccag tgaataatgc gtcgatccac ggaccgccaa actcgcctgg cggcaggcta
     3001 atattgtgca ccaccagcag ggtgggtgtt tcgtcatccg ggcggcaatc gtaatgtggt
     3061 gagggaacgc ggcgcgcgcc aaccagccac ccctgttcta acaacatgca ggatctcctt
     3121 atatgtggtg ctaatacccg gttcagagta gcatgtttct accttatgat tcgttagcta
     3181 tctggagttt taacatgccg cctcgccgct ataaccctga cacccgacgt gacgagctgc
     3241 tggaacgcat taatctcgat atccccggcg cggtggccca ggcgctgcgg gaagatttag
     3301 gcggaacagt cgatgccaac aatgatatta cggcaaaact tttaccggaa aattctcgct
     3361 ctcatgccac ggtgatcacc cgcgagaatg gcgtcttttg cggcaaacgc tgggttgaag
     3421 aggtgtttat tcaactggca ggcgacgatg tcaccataat ctggcatgtg gatgacggcg
     3481 atgtcatcaa cgccaatcaa cccttgttcg aacttgaagg cccatcccgc gtgctgttaa
     3541 cgggcgaacg cactgcgctt aattttgtgc aaaccctttc aggagttgcc agtaaggtac
     3601 gccactatgt cgaattactg gaaggcacca acacgcagtt gttggatacg cgcaaaacct
     3661 tacccggcct gcgttcagct ctgaaatacg cggtactttg cggcggcgga gcgaatcacc
     3721 gtctggggct ttctgatgcc ttcctgatca aagaaaacca tattattgcc tccggctcag
     3781 tgcgccaggc ggtcgaaaaa gcgtcctggc tgcacccgga tgcgccagta gaagtcgaag
     3841 tagagaatct ggaagaactt gatgaagccc tgaaagcagg agccgatatc atcatgctgg
     3901 ataacttcga aacagaacag atgcgcgaag ccgtcaaacg caccaacggc aaggcgctac
     3961 tggaagtgtc tggcaacgtc actgacaaaa cactgcgtga atttgccgaa acgggcgtgg
     4021 actttatctc cgtcggtgcg ctaactaaac acgtacaagc actcgacctt tcaatgcgtt
     4081 ttcgctaata gttgacagat ttatccgctc catcgcgggc ggataatctc cccttcccca
     4141 actttcttcg taacgcctcg caaatttctt gtctttcgct gcaacgaaat aaaaaacctc
     4201 ggaagctgct ttcccgtttc gttttttgcc cctcgcttgt gctgatggcg tttgccaaag
     4261 tagcgccaac caaatcaatg agcgaaacag atggacaagc aacgcggttt tacacttatc
     4321 gaactgatgg tggttattgg catcattgcc attttaagcg ccattggtat tcccgcttat
     4381 caaaactacc tgcgcaaagc tgcactcacc gacatgctac aaacctttgt gccttaccgt
     4441 accgccgtag agttgtgcgc gctggaacat ggtggattag atacctgcga cggtggcagc
     4501 aatggcattc cctcgcctac caccacccgc tatgtttcag ccatgagtgt gacaaagggc
     4561 gtggtgtcgc tgaccgggca agaaagtctc aatgggctaa gcgtcgtcat gacgccgggt
     4621 tgggataacg caaacggcgt caccggctgg acgcgcaact gcaatattca aagtgacagc
     4681 gcattgcagc aagcctgcga agatgtcttc cgctttgatg acgccaacta aggagcggca
     4741 atgaatattc cacagctcac tgccctgtgt ctgcgttatc agggagtctt gctggatgcc
     4801 agcgaagagg tggttcatat tgcggtagtc gatgcacctt cgcatgagct actggacgca
     4861 ttgcatttcg ctaccaccaa acgtattgag atcacctgct ggacgcgcca acaaatggaa
     4921 ggtcacgcca gtcgcacaca acagacattg cccgtagctg ttcaggagaa gcatcagccc
     4981 aaagcagagt tgctgactcg aacgttacaa tctgcgctgg aacaacgcgc gtctgatatt
     5041 catatcgaac cagcggacaa tgcctaccgc atccgcttgc gtatcgacgg cgtattgcat
     5101 cctttaccgg atgccggagt cgcattaacc gccagattaa aagtgctggg aaacctggat
     5161 attgcggaac atcgcctgcc gcaggacggg caattcactg tcgaactggc aggaaacgcc
     5221 gtctcatttc gtattgcgac cttaccatgt cggggtggtg aaaaggtggt attaaggttg
     5281 ttacagcagg tgggtcaggc actggatgtc aacacgcttg gaatgcagcc gttacaactg
     5341 gcggactttg ctcatgcctt gcaacaacca cagggactgg tgctggtaac tggccctacc
     5401 ggcagcggca aaacggtcac gctttatagt gccctgcaaa agctgaatac cgctgacatt
     5461 aatatttgta gcgtcgaaga tccggttgag atccccatag ccggactaaa ccagacgcaa
     5521 atccatccgc gtgccggact cacctttcag ggcgttttgc gtgcgttatt gcgccaggat
     5581 cctgacgtca tcatgatcgg agagatccgc gatggcgaaa cagcagagat cgctattaaa
     5641 gcggcgcaaa ctggtcacct ggtgttgtct accctacaca ctaattccac ctgcgaaacg
     5701 ctggtacgtt tacagcaaat gggggtcgcc cgctggatgc tatcatcggc gcttacgctg
     5761 gtaatagccc agcgtctggt acgcaaactt tgcccacatt gtcgccggca gcaaggggag
     5821 cccatccaca ttccagacaa tgtatggcca tcgccgctgc cccactggca ggcacccggt
     5881 tgtgtacatt gctaccacgg tttttatggt cgtacggcct tatttgaagt tctgcccata
     5941 acgccggtca ttcgtcagct tatttccgct aataccgacg ttgaatcgct ggaaacgcac
     6001 gcacgacagg cgggtatgcg tacgcttttt gaaaacggct gcctggccgt agagcaaggc
     6061 ttaaccacct ttgaagagtt aatccgcgta ctggggatgc cgcatggcga gtaagcaact
     6121 ctggcgctgg catggcataa ccggcgacgg caatgcgcaa gatgggatgc tatgggcaga
     6181 gagccgtact ttactgctta tggcactaca gcaacagatg gttaccccac taagcctgaa
     6241 gcgaatcgcc atcaattctg cgcagtggcg aggagataaa agcgcggaag tcattcatca
     6301 actggcgacg ctactcaaag cagggttaac gctttctgaa gggctggctc tgctggcgga
     6361 acagcatccc agtaagcaat ggcaagcgtt gctgcaatcg ctggcgcacg atctcgaaca
     6421 gggcattgct ttttccaata ccttattacc ctggtcagag gtatttccgc cgctctatca
     6481 ggcgatgatc cgcacgggtg aactgaccgg taagctggat gaatgctgct ttgaactggc
     6541 gcgtcagcaa aaagcccagc gtcagttgac cgaaaaagtg aaatcagcgt tgcgctatcc
     6601 catcatcatt ttagcgatgg caatcatggt ggttgtggca atgctgcatt ttgttctgcc
     6661 ggagtttgcc gctatctata agaccttcaa caccccacta ccggcactaa cgcaggggat
     6721 catgacgctg gcagacttta gtggcgaatg gggctggctg ctggtgttgt tcggctttct
     6781 gctggcgata gccaataagt tgctgatgcg ccgccctacc tggcttatcg cgcggcagaa
     6841 attgttgtta cgaatcccga ttatgggttc actgatgagg ggacaaaaac tcacgcagat
     6901 ctttacgatt ctggcgctga cacaaagtgc aggcattact tttttacagg gcgtagagag
     6961 cgtcagagaa acaatgcgct gcccgtactg ggtgcaactt ctgacacaaa tccagcacga
     7021 tatcagtaac ggtcatccca tctggctggc gctaaaaaat gccggtgagt ttagcccgct
     7081 ctgtttgcaa ttagtgagaa cgggagaggc atccggctcg ctggatctca tgttagacaa
     7141 cctcgcccat catcatcggg ataacacaat ggcgctggcg gataacctcg cagccttact
     7201 ggaaccggcg ttgctgatca taacgggagg aattatcggt acgctggtgg tggcgatgta
     7261 tctgccaatt ttccatttag gcgatgcgat gagtgggatg ggataacgct ggcgtgttgc
     7321 tccacgccag cgttgggaga ttacaggttg ttgaagatgc ggttttcttg ttcctgcacc
     7381 cgaataaacg tggtgcgctt ggtcagttct ttcaggcgtg aagccccaac gtatgtacaa
     7441 gctgaacgca agccgcccaa aatatcacgc gcggtatttt caaccgggcc tcgcagcggc
     7501 agcttaacgg ttttaccttc tgctgcgcga tattccgcaa cgccgccaac gtgacgtttc
     7561 atcgcagact cggagctcat gccgtagaac agcataaatt tctcgccgtt ctcctcaacg
     7621 atgcgaccac cgctctcttc gtggcccgcc agcatgccgc caagcatgac gaaatcggca
     7681 ccgccgccaa aggctttcgc gacatcgccc ggcgtggtgc agccaccgtc gctgacgatc
     7741 attccgccca gaccgtgcgc cgcatcggca cattcgatta ccgcagaaag ctgcggataa
     7801 ccgacgcctg ttttgacacg agttgtacaa acagaacctg ggccaatgcc aactttaacg
     7861 atatcagcac cggagaggat aagctcctca cacatttcac cagtcactac gttgcccgca
     7921 caaatggttt tggtcggcca cgcttcacgc gctttcgcaa cgaactgcac gaagtgttcg
     7981 gaataaccat tcgccacatc tatacaaacg aagtttaatg ccgggttcag gtcgagaatc
     8041 tgtttagttt tttcgaaatc cgcatcagac gtaccggtag aaaccatcac atgtttcagc
     8101 acatcagcgg aagaattgtt gataaacgct tgccactctt cgacagaata gtgtttatgc
     8161 acagcagtca aaatatcaaa agaagccagc gcagaggcca tagaaaatgt acctacggtg
     8221 tccatatttg cggcgataat cggcacgccg gaccagctct gacctgaatg tttgaaggtg
     8281 aattgacgtt ccagttcaac atcggaacgg cttttaagag tggagcgttt agggcggatg
     8341 agaacgtctt taaaacctaa cttcagatct tcttcaatac gcatgtgcgg attcctgggg
     8401 ttaatggcta taagggtaag agcgcaactc cagtgacgct atcatacgca ctaatcaatg
     8461 cggcgcaaga ctgcgaaatt gtcttttttt acgctacaat cccataaatt tacctggtaa
     8521 atagtgagcg aacgtttcat caatttcgct ttcgtgcgct catttttaac gttttgataa
     8581 tcatgatgaa ttcccgggaa tataaattat gaggtatata gttgccttaa cgggaggcat
     8641 tggcagtggc aagagtaccg ttgccaatgc gtttgctgat ctcggaatta acgtcattga
     8701 tgccgatatt attgcgcgtc aggtggttga accaggtgca cctgcgctac atgccattgc
     8761 tgatcacttt ggcgctaaca tgattgctgc tgatggaaca ttgcagcgcc gggccttgcg
     8821 cgagcggatc ttcgccaacc cggaagagaa aaactggctt aacgacctgc tgcatccgct
     8881 gattcagcaa gagacgcaac accagatcca gcaagcaact tccccctatg tactgtgggt
     8941 tgtgccattg ctggtagaaa actcactgta taaaaaagcg aatcgagtgc tggtggtgga
     9001 tgtcagccca gaaacgcaac ttaagcgcac catgcagcgc gatgatgtaa ctcgcgaaca
     9061 tgtcgaacaa atccttgctg ctcaggcaac gcgcgaagcc cgccttgccg tggcagatga
     9121 cgtcattgat aataacggcg caccggatgc tatcgcatcg gatgttgccc gcctgcacgc
     9181 acactatttg cagcttgcgt cgcagtttgt ctcacaggaa aaaccgtaat gcagacccag
     9241 gtcctttttg aacatccact aaatgaaaaa atgcgtacat ggctgcgcat tgagtttttg
     9301 attcagcaac tcaccgttaa tttacccatc gttgaccacg ctggcgcgct gcatttcttc
     9361 cgtaatgtca gtgaattact ggatgttttc gagcgcggcg aagtccgcac tgagctgttg
     9421 aaagaacttg accggcagca acgtaaactc cagacttgga ttggcgtgcc tggcgtggac
     9481 cagagccgta ttgaagcatt aattcagcag ttaaaagcgg cggggagcgt attaatttcc
     9541 gcgccgcgta tcgggcaatt tctgcgtgaa gatcgtttga ttgctctggt gcgtcagcga
     9601 ctgagcatcc caggcggctg ttgcagcttt gatttaccta cattgcacat ttggctgcat
     9661 ctaccccagg cgcagcgcga cagccaggta gaaacctgga ttgccagcct gaacccgctc
     9721 acccaggcac ttaccatggt gctggattta attcgccagt cggccccctt ccgtaaacaa
     9781 accagcctga atggttttta tcaggataac ggtggcgatg ccgacttgct gcgcctgaat
     9841 ctgtcgctcg attcacagct ttatccgcaa atttccggac ataagagccg ttttgccatt
     9901 cgttttatgc cgctggacac tgaaaacgga caggtaccgg aacgtctgga tttcgaactg
     9961 gcctgttgct aaggagtgaa aatgtcagaa actattacgg tgaattgccc aacctgcggg
    10021 aaaacggtgg tgtggggtga aatcagcccg tttcggccat tttgctccaa acgttgtcag
    10081 ctgatcgacc tcggagaatg ggctgctgaa gaaaaacgaa tccccagcag tggcgatctt
    10141 tccgaaagcg atgactggag cgaagagcca aagcagtgac atttgtctga tgccgcacgt
    10201 aggcctgata agacgcgcca gcgtcgcatc aggcatcgtg tgcgaatgtc ggatgcggcg
    10261 tgaacgcctt atccgaccta cagacgctta agcttcgcaa ttaccggttc attggctggc
    10321 ggaaaatcat cggcattaag accgaccagc gacatccact caccgggttg cccttcttta
    10381 ccccacggca ccccttccca actttcgacc agccagaacc acagagttat atgcctgtcc
    10441 gggaattcat atgccagttt ttcaaatagc gaaaaatgtt gtggggtaat cccgacctct
    10501 tcctgaagtt cacgcaccac cgcctgttcc ggcgtttcgc ccatttcaat tttaccgccg
    10561 ggaaactcca gtttattcgc catgtgcgca tctgctgcgc gacgcgttat aaagatttca
    10621 ttgttctcgt tgcgaataat acctaccgca atttgcagct ttttcattgt tttaaaccta
    10681 taaaaaaggc gcagaatcct gcgccttttg cttcaacagt tagcttttat tgcaggcggc
    10741 catgacactg cttgtatttt ttaccagaac cgcacgggca aggatcgtta cgtcctactt
    10801 tgcgctcacc ggtttgcgcc gccagtgcag ctgcggctgc agagtcgtca tcctgatggc
    10861 taagctgctg catttgcgct aaacgctcgg cttccatacg acgctgttgt tccagctcct
    10921 caacctcttc aggcatacgt acctgaactt tgctcagcgt actgataact tcatatttca
    10981 acgactccag catcgctgca aacatggaga acgattcacg tttgtattcc tgcttcggat
    11041 ctttctgtgc atagccacgc aggtggatac cctgacgcag atagtccatc gccgccaggt
    11101 gctctttcca cagagagtcg agagtttgca gcatgacgcc tttttcgaag tgacgcatca
    11161 tctcagcacc aaccacttct tctttacgct gatacacttc gatggactgc gccagaattc
    11221 gctcacgcag cgtctcttca tgcagttctg gttctttatc cagccactcg gcaattggca
    11281 aatcgaggtc gaaatcgttc ttcagacgtt cctgcagccc cggaatatcc cacatttctt
    11341 ccagcgactg tggcggaatg taggcatcaa tggtggcttt gaacacatct tcacgaatgc
    11401 tgttgatggt ttcgctcaca tcgctgacat ccagcagttc gttacgctgg gagtaaatgg
    11461 cgcggcgctg atcgttagcc acgtcatcat attccagcag ttgcttacga atgtcgaagt
    11521 tacgactttc aactttacgc tgggcgttgg caatcgcttt agtcacccac gggtgttcaa
    11581 tggcttcgcc tggcttcata cccagtttac gcatcattcc ggatactcgg tcagaagcaa
    11641 aaatacgcat cagcgcatct tccatcgaca ggtagaaacg ggaagaacca gcatccccct
    11701 gacgaccaga acgaccgcgc aactggttat cgatacgacg ggattcgtga cgctcggtac
    11761 cgatgatatg caggccacct gcttccagta ccgcgtcgtg acgtacctgc cagtcggctt
    11821 taattttttc aatttgctct gcggtcggat tttccagcgc ggcaacttct gcctgccagc
    11881 taccaccgag cacgatatct gtaccacgac ccgccatgtt ggtcgcgata gtcaccgcag
    11941 ccggataacc tgcctgagca acaatcgccg cttcgttggc gtggaatttg gcgttcagga
    12001 cattgtgctt aataccggct ttggtcagtt cgtttgacac cagctccgat ttttcgatgg
    12061 agatagtacc caccagcacc ggctggcctt tcgcagtacg ttctttgata tcttcaatga
    12121 tcgcctgaat tttttccgct tcagtcatgt agaccaggtc cggcagatct ttacgaatca
    12181 ttggacggtt ggtcggaaca acgacggtat ccagtttgta gatggagcta aattcgaaag
    12241 cttcggtatc agcagtaccg gtcatccccg ccagtttttc atacagacgg aagtagttct
    12301 ggaaggtgat cgaagccagc gtttggtttt cgttctggat ctgcacacct tctttcgctt
    12361 ccacagcctg gtgcagacca tcggaccagc gacggccctg catggtacga ccggtgtgtt
    12421 cgtcaacgat gataacttca ccatctttaa cgatgtagtc gacgtcacgg gtaaacagcg
    12481 catgagcgcg cagcgccgcc gttacgtggt gcatcagcat gatgttggcc ggagagtaca
    12541 gagactcccc ttcatccatg atgccctctt tcaccagcag ttcttcaatc agcaccagac
    12601 cacgttcggt caggttcacc tggcgagatt tttcgtccac cgagaagtgg ccttcgccct
    12661 ggaaggtttc ggagtcttct ttttcctgac ggatcaggtg cggaataatt ttattcacgc
    12721 gtttatacat ttccgagctg tcttctgccg ggccggaaat gatcagcggt gtacgcgctt
    12781 catcgatcag gatggagtcc acttcgtcca ccagcgcata gtgcagttta cgctgtacac
    12841 gttcttcagg gctgaacgcc atgttgtcgc gcaggtagtc aaagccgtat tcgttgttcg
    12901 taccgtaagt gatgtcagct gcataagctt cacgctttgc cggtgctggc atgcccggca
    12961 ggttaatacc aacagtcagg ccaaggaatt caaacagcgg acggttgttt tcggcgtcac
    13021 gttgcgccag gtagtcgttg acggtaacta cgtgcacgcc tttaccggtt agtgcgttca
    13081 ggtaagcagg cagcgttgcg gtcagggttt taccttcacc ggtacgcatt tcggcgatgc
    13141 agcgttcgtt aagaaccata ccgccgagta actgaacgtc gaagtgacgc ataccaaaga
    13201 cgcgcttact tgcctcacgt accacggcga aagcttccgg gatcagattt tccagcactt
    13261 cgcctttttc cagacgcgca cgaaactctg cggttttccc tttcagttct tcgtcggaga
    13321 gtttttccat ctccggttcc atggcattga tgatgttgac cactttgcgc atccggcgca
    13381 gggtgcgatc gttacgacta ccgaaaactt tagttaataa tttgattagc ataataaaat
    13441 ctcaaacgcc ccgcgttgcg gagttaataa aatgaagtaa aggtttattg ttgttagctg
    13501 aggcgttgag ggccagcacg gatgccttgc gcctggctta tccagacggg cgtactgaat
    13561 tttgcttgtg gggtaaaatg cgcataatca atgcgataac ccttttcaga cggcgtgcct
    13621 tcctgggtca gcagcgcgct gagcgtatcc agtaatgcaa gatgttgcgc ctgaagaggc
    13681 aaagattctt cagcaacggg cagtgtttgc ggtgccattg cgaaagaaag atgacggatt
    13741 accgtgcgaa tagcatgttg atgccagtaa tcaacggaat agttcgaatt agggcggcgc
    13801 gtgttcgctt ccagcaaggc caattgacca aagttaactt tggctgaagg ctcgtggttg
    13861 cgggttgtcg cttttgcggg cgcgtttggt tcggcggcgt tgctgagcgc aggcaaacct
    13921 aaactcgccg cgaccatccc taataagaga tgcggccaga agtagcgttt accaaactgt
    13981 cgccagcgcg tcagtattcc actcacgtta ttgccatccc gatcgaattc aaacgtattt
    14041 agcgcacaaa tcttatcatt aaagcctgaa ctccacagca cgaatgaaga taagttgtgc
    14101 gtaaaagtgc ttaagaaaac agcgttcgca ccgtttttta tagctcttat aaaattcaaa
    14161 aaaaaacgac tggttcacct ggccggagag agtgccagat ttgccagtcg aattttatac
    14221 gacagtataa atgtcgttat gccagtacag ctgaaggcgc tttgaaggcc aacggcagtt
    14281 ctgcgtcgtc ctggaaggtc acatattccc aggcttcctg tttcgccagg acagcctgca
    14341 gcagtttgtt attcagtgca tgaccggatt tataagcggt aaatgcacca ataatattgt
    14401 gaccacacat gaacaagtca ccgatcgcat cgagcatttt gtggcgcaca aattcgtctt
    14461 caaaacgcag gccgtcttcg ttcagtacgc gataatcgtc aacaacgatg gcacaatcga
    14521 agctgccgcc caggcacaaa ccacgggact gcagatattc gatatcacgc atgaaaccga
    14581 acgtacgcgc acggctgatc tggcgcataa acgcatcagc ggagaagttc atcgcatagc
    14641 gctggttgct ggaatcaata gccggatggt taaaatcgat ggtgaaatcc agcgaaaaac
    14701 cattgtacgg cttaaattca gcccacttat cgccatcttc gacacgaaca gtctctttga
    14761 tgcgaacaaa tttcttggcg cagttcaact cgtcgatacc ggcgtcaagc agcaggtata
    14821 caaacggagc ggcgctgccg tccatgatcg ggatttccgg cgcgttaact tcgataacaa
    14881 tgttatcgat gcccaggccc gcgagagcag cattgaggtg ctctacggtt gaaatccgta
    14941 catcatgctc gttgaccaga cacgtacaga gcattgtatc acgcacagat ttggcatcgg
    15001 ccgggaaatc taccggtgga ttcaagtcgg tgcgacgata gatgaccccg gtgttggccg
    15061 gcgcagggcg taacgtcagg gtgactttct tgccggtatg taaaccgaca cccgtcgcct
    15121 gaacgatacg tttaagtgtc ctttgtttga tcatcgtatt atctcgccaa attacctatc
    15181 caaccgaagt gtactataca ttcggcgggc cagtttagca caaagagcct cgaaacccaa
    15241 attccagtca attcttaatc agcttgctta cgcaggaatg ctgggatatc cagataatcc
    15301 ggctctttcg cagtttgcgg cgcattgtca ttcacgactt tagcaaccgg cttctgctcc
    15361 tgggtcagcg gagccatccc atgctgctgg tagcgatcca tcactggctg ctgaacctgc
    15421 ttattggtca ccagagtgat ttcaggacgt ttgtccatgc cgatacctgt cgcaacaacg
    15481 gttacgcgca gctcgtcatt catatccggg tcaagagaag taccgataac cacagtcgcg
    15541 ttgtcggaag caaatgcacg gatggtgtta cctaccgttt cgaactcatc cagacgcagg
    15601 tcgaagcccg ccgtgatgtt aaccagcacg ccgcgcgcgc cagacaggtc gatatcttcc
    15661 agcagcggag aagagatagc catttcagca gcttcttccg cacggtcttc accgctcgcc
    15721 acgccagaac ccatcattgc gtagcccatt tcagacatta cggtgcgtac gtctgcaaag
    15781 tccacgttca tcaaacccgg acgagtaatc agttcagcga taccttgcac agcgcctttc
    15841 agtacatcgt tcgctgcgcc aaacgcatcc agcagggaga taccgcggcc cagaactttc
    15901 agcagtttgt cgttcgggat agtgatcaga gagtccacat gcttggacag ttcagtgatc
    15961 ccctgctccg cgaatgccat acgcttcttg ccttcaaagt tgaaaggctt agtgacgaca
    16021 gcaacggtca ggatacccaa atcttttgcc acttcagcga cgactggtgc tgcacctgta
    16081 ccggtaccac cacccatacc cgcagcaata aagaccatgt ctgcaccttc cagcgccgca
    16141 cgcagtgcat cgcgatcctc atcagccgca ttgcggccaa cttctggatt agcgccagcg
    16201 cccagtcctt tggtgatacc gctaccgatt tgaatcgtct gtccaaccgc tgttttacgc
    16261 agcgcttgtg catcggtatt taccgcgaag aattcaacac cttcaatgcg ctcgcgcacc
    16321 atgtgttcaa cagcattacc gccgccgccg ccgacgccga tgactttaat caccgcgtca
    16381 ttggtaagtt ccattggttc aaacatagtt tctctccgat ttgtgcctgt cgcctgaggc
    16441 cgtaatcatc gtcggcctca taaaaattaa aactcttttc gcagccaact attgagtcgc
    16501 ttgatccacg agccaactga tgctgtaaca cgtttttcta cttcagcttc accgttaaga
    16561 tgtgactctt tcccatagtg aagcaatccc accgccgtcg aataatacgg ctcctgagca
    16621 taatccgtta aaccggtaat gttcagcggc gcgccgatac gcacttgcgt atgaaacacg
    16681 cgctgagcac aggctgcaag accttcgatc tgcgctgcgc caccggttaa tacaatgcct
    16741 gccgccaggt gatgtttaac cccttgttgg cgaagctttt cctgcaactg caatatctct
    16801 tcgttgacca ggttgagcag ctcggtatag cgcggctcga tcacctctgc cagggtctga
    16861 cgttgcagac tccgcggcgg acgaccgcct acgctcggca cttccacgct ctcatctttt
    16921 ccaacgatgg aacccagcgc acaaccgtgg cgaactttaa tcgcttcggc gtcacttggc
    16981 ggcgtgccaa aggcgtaagc gatatcactg gtcacgacat tgccagcata aggaattacc
    17041 ttagtgtggc gcaatgcccc accggtataa acggcgatat ccattgtacc accaccgata
    17101 tcgacgacgc agacacccag ttcacgttca tcttccgtca ataccgaata acttgatgcc
    17161 agcccagcaa atatcagttg gtcaactttc agcccacaac gttcaaccgc tttgacgatg
    17221 tttttcgcca tatcgttgtg acatgtgatc aggtgcactt ttgcctgcat ccgcacgccc
    17281 gaaagtccta ccggattctt gatcccttcc tgatagtcaa tcgcatactc ttgcgggatc
    17341 acatgcagca cacgatgctc atcgcgcaca cgcaccgatt tcgcggtatg gacgacgttt
    17401 tccacatctt cttgcgtcac ttcttcttca gaaataggca ccataccaat ttcattctgg
    17461 cagctgatgt gcttaccaga aagcgccaga tataccgaag agatctgaca atctgccatc
    17521 aattctgcct ggtcaatggc gcgttgtacg cacttgacca cggattcgag gtcgttcacc
    17581 ccgcctttat ccataccacg tgacgggcag ctgcccacgc caatgatatt gaccataccg
    17641 tcgggcagaa cttcccctac taaagcggca accttcgcgg taccaatctc cagtcctact
    17701 accagttttc tgtccgtcgc cttgatcatt gttgttctgc ctgtgcctga ttttgttgct
    17761 gagtagattc ctctggcggc aagggcgccc agcctactgc cgctccagag tcataacgca
    17821 aatcaacgta gctaatccgt ttgccatcgg tttgcgcctg ctgctgtaaa accggataaa
    17881 gttctacaaa gcgagccaaa cgtttcatcg tatcgccccg gccaagattg agcttaatat
    17941 cgttattcag cgtcaactgc caggaacgcc gcgcggtcat cgccgcttcc ttcagagtaa
    18001 atctgtcctt tgccagcatc tgccccattt cgcgatagcc ctgcaacact tcattggcgc
    18061 tgccttccgg gccatacagt attggaagca cctgcttgct ggtgcgatct ggcggcacgc
    18121 tgaaggtgtt tccttccgcg tctaccatgt gttgatcatt ccaccgcgca atcggcacat
    18181 attcaaccag atgaatcttc aattcatcag gccactgctt tctgacgctc acctgcttaa
    18241 tccacggcag gcgttgttct atttgcgtct ggatgatgtt gacatcctgg gtcataaagg
    18301 tacccggctc acccaatgcc aggatcgact gccggatatc gtcattacgc gtgtaatggc
    18361 gttcaccggt caacaccagc tttgagagcg gcaggcgttg cgcatcttcc atccagccca
    18421 acacgaccca gccgctcacc aacactgtcg ttaaaacggt cagcaggaaa aggatccccg
    18481 ccagacgcgt tccattattg cggcgagaag aaacctcttc ttcgctgttt cgcgtgttca
    18541 gagcagcctg cgacatatta gtccgccagt tccagaattc gtactaccaa ctgcgagaag
    18601 ctcatacctg cctgacgtgc cgccatcggc accaggctgt ggctggtcat acccggtgag
    18661 gtattggctt ccagcagata aaactgtcca tcgctgtcca gcataacgtc aatacgtccc
    18721 catcctttgc aacctaacgt cgtccatgct ttcagcacta atgcctgcaa attggcctct
    18781 tgtgacgctt ccagacctgc ggggcagaaa tactgtgtct catcagagag atacttcgcc
    18841 tcataatcat agaaggttcc ggacggttga atacgtattg acggtaaaat ttcttcaccg
    18901 agtatcgcaa ccgtgaactc cggcccactt agccattttt caatcaatac ttcttcatcg
    18961 tgctgaaatg ccaatcttaa tgcatcttgt agagcatttt ctgctactac ttttgacatt
    19021 cccacactgg aaccttcgcg gctcggctta acgataaccg gcaaacccag agcagaaatt
    19081 tctgctaact gcttatcact caggcctttt tcaaactctg cgcgggttaa cgctacccac
    19141 ggagcgaccg gtaaaccggc accttgccac agaagtttgc tgcgtagttt atccattgaa
    19201 agcgcagatg ccatcactcc gctgccggta taaggcaagc ccatcagctc gagcatgccc
    19261 tgcagcgtgc catcttcacc gccgcgaccg tgtagcgcga taaacacttt ctgaaagccc
    19321 atcgacttca gttgcgtcac gtcgacttct ttcgggtcga caggatacgc gtcaataccg
    19381 ccttcacgca gcccggctaa caccgctgcg ccagaattca gagaaacttc ccgctcagcg
    19441 gaggtcccgc ccaacaggac cgcgatttta tcagtcatgt tgttcttcct ccggagtttg
    19501 cggcttcagt ttgatttcag ctaaagaacg ggcaattttt ccgatattac cagccccctg
    19561 aacgagaatc aggtcgttac cggttaatac cggtgccagc atctcggcta cctgcgccgg
    19621 atccggcacc agaatgggat ctattttccc acgtccacga attgtgcgac acagcgaacg
    19681 gctgtccgct cccggaattg gcgcttcgcc agccggatac acttccagca tcaacagggt
    19741 atcaacctgc gtcagcacat tggcgaaatc atcatacagg tcgcgcgtac gggtaaaacg
    19801 gtgcggctga aacagcatta ccaggttttt atccggccag cctgcacgcg ctgctttaat
    19861 agtggcgtcc acttccgtcg ggtggtggcc gtagtcatcg accagcatcg ccgtaccgct
    19921 tttaccattc actgactcca gcgggaattc accgaggaaa tcaaaacggc gaccagtccc
    19981 ctggaagctc tccagcgcac gcaaaatagc ctcgtcgtca atgccctctt ccgtagcaac
    20041 cgcaaccgca gctgcggcgt tcagcgcgtt atgacgacct ggcgcattca gggtgacgcg
    20101 catcggctct ttgtcctggc gcagcagcgt aaagtgcccc tgcgggccaa tctgctgata
    20161 atcttctaca cgcacgtcgg catcttcgct gaagccgtaa gtcgtggtct gacgtcccac
    20221 acgcggtaac aattcgcgga tcacaggatc atcaacacac atcaccgcac gaccgtaaaa
    20281 cggcaggttg tgcagaaaat taataaaagt ctgttttaaa ttctcaaagt cgccctggta
    20341 ggtatccatg tggtcggctt cgatattggt gacaatcgcc accatcggtt gcagatgcag
    20401 gaacgatgca tcactctcat ctgcttcggc aatcaggtac cgaccatgcc ccaaacgcgc
    20461 atgaaccccc gccgctttta ccagcccgcc gttaacgaag gttgggtcga gcccagcttc
    20521 tgcgtagatg ctggaaacca tcgcggtggt tgtcgttttg ccgtgcgttc cggcaatggc
    20581 gatgccatga cgaaaacgca ttaactcagc cagcatttcg gcacgacgga tcaccggaat
    20641 acgcgcttca tgagctgcga caatttccgg gttatcggca gaaatcgcgc tggaaacaac
    20701 gaccacgctg gcatcacgta cgttttccgg gcgatggttg aaataaatcg tcgctcccag
    20761 attcattaac tgttgcgtga ccggatttgg cgctaaatcg gaaccactga tctgataacc
    20821 ttcattggcc agaacttcgg caataccgcc cataccggca ccaccaatgc cgacaaaatg
    20881 tatgtgccga acgcgacgca tttcgggcac gatggaacgc agttttgcca attgttgtgt
    20941 attcattctt tacgccatta acttcttaaa ttcatacgat gcaaaaggca tcgctacaat
    21001 tacgcccggg cagcccggct cacttcattt gccactcgct cggtggcatc cggaatggat
    21061 gcggcgcggg cgcgttctgc catggttaat aaggtttctc gcgaccaccc ggccagggtg
    21121 ttggcgacag catccacgct aagctgtggc tgctcgataa ttttggctgc gcccgctttt
    21181 tccagcggta gcgcattcca gtattgctgg cggtctttat gttgaaacgg cacaaacaac
    21241 gccggtagtc ctgccgcggc gatttcactc accgttaacg caccggagcg gcaaacgacg
    21301 acatccgccc acgtatacgc cgccgccata tcatcaataa attccgtcac tttatgctgc
    21361 ggttgccccg cttcggcata cgcctgttca acggattgtt gcgaaccttt gccgctctga
    21421 tgccagatag tgactgaatc acccagtttc gcagcaacct gcggcattgt ctggttaaga
    21481 atgcgtgcgc cctgggaacc acccactacc agcacacgaa ccggaccttc acgtccagcc
    21541 aaacgttgct gcggcaacgg tagcgccaac acatcggtac gcaccgggtt acccaccact
    21601 tccgcattag ggaaagcacc tggaaacgcc tgcatcactt tggtggcaat cttcgccagc
    21661 catttattgg ttaagcccgc aataccgttt tgttcatgaa gcacaaccgg aatgcctaac
    21721 gaccacgcgg ccagaccacc tggacctgat acgtagccgc ccataccgag caccacgtca
    21781 ggtttgtacg ctttcataat cgcccgcgcc tgacgccagg cgttgaagat acgcagcggg
    21841 gcagctatca gtgcttttat accttttcca cgcagaccag agatacgaat gaaatcaatt
    21901 tcgatgccat gttttggcac taagtccgct tccatacggt cggcagtccc cagccagcga
    21961 acttgccaac cctgagccat tagatggtgc gcaaccgcca gtcccgggaa tacatgtcca
    22021 ccggttccgc ctgccatcac cattaatcgc tttccttgac cactcatcgt gaacctcgta
    22081 caaacgcctg cgctttctcc agacgcgttt cataatcaat acgcaacagc atcatgatgg
    22141 ctgtcgacat aatcagtaag ctcgaaccac cgtaactgat tagcggcaat gtcagacctt
    22201 tagtcggtaa catccccgcc gccgcgccta cgttaaccag cgcctggaag ctaaaccaga
    22261 tgccaataga acaggcgaga aaaccggaaa aacggtggtc aatttctaat gctttacggc
    22321 caatcgacat cgcgcgaaaa gcgacgaaga ataccattaa aagtgccagc accacaccga
    22381 cataccccag ttcttcgccg ataatggcga aaataaagtc agtgtgcgct tccggcagat
    22441 actccagttt ttgtaccgag ttacctaaac cttgccccca aagttcgccg cgaccaaacg
    22501 ccatcagcga ttgcgttaac tgatagccgc tgccaaaggg atcttcccac gggttccaga
    22561 atgcggtaac acggcggata cggtacggtt cggcgagtat cagcaacaca accgctgaaa
    22621 tgcccatacc gataatggca atgaactgcc acaatttcgc tcccgccagg aacaacatcg
    22681 ccagcgtagt cacaaacaac accaccaccg taccaaggtc tggctgtgcc agcagtaaca
    22741 ctgccaacac cagaatcacg cccatcggtt tcaggaagcc gcgcaggtta ttacgtactt
    22801 cgtcgccttt acgcaccaga tagttggcga tatagcaaaa cagcgacagt tttgtcagct
    22861 ccgcaggctg gatacgcagc aaaccgagat cgatccaacg cgatgcccct ttaacagagc
    22921 tacccactac cagcacgatc atcagcagga tgatagatcc gagcagcatc gtggcactgt
    22981 agcgttgcca gaactccatc ggcagacgca gcgtaatgat cgccagaatg aatgccagga
    23041 tcagatagac gccatcacgc ttcgcgaaga agaacggatc gttggttaag cgttgcccta
    23101 tgggcattga cgccgaggtc accatgataa agccaatcgc cgcgaggccg aaggtcagcc
    23161 acagtaaggt gcgatcgtac atgatcaggc tgtcggtatc tttttcccgc gagcccatca
    23221 cccagccctt tagcgccgtg gagatccaga ccaggatact gaatcctggc aggcgcggca
    23281 ttttcaggcg agggagagat aaacgcatca acctaactcc ttcgccagac gggcaaactc
    23341 attgcctcgt tgttcaaagt tcttgaactg atcaaggctg gcacaggccg gggagagcag
    23401 aaccatatcg cccggctgaa cacgcggagc cagcaagcgc atcgcctgtt ccatagtttc
    23461 ggtttgttct gccacttccg ggcgtagcgc cgccaactgc gcgccgtcac gaccgaaaca
    23521 atacagacgt acgttatcgc cattcaggta acgcgccagt gggctaaagt ccgccgattt
    23581 accatcgcca cccagcaaca aatgcagcgt gccgtctacg tgcaggccat tcagcgccgc
    23641 ttccgtactg ccgacgttgg tcgcttttga atcgttaatc caacgtacgc cgttatgctc
    23701 cagcacaact tcaaagcgat gcggcagacc ggtgaatgtg gttaacgctt tcaggctgct
    23761 ggcgcgcggc aaaccagctg cgtctgccag tgccagtgct gcgagcgcat tggtgtagtt
    23821 atgctgcccg gaaagtttca tctctttcac gttcagcact ttctcgcctt tcacccgcag
    23881 ccaggtttcg ccctgctgat gattcaggtg atagtcaccc atgttgacgc caaagctgac
    23941 gcagcgttca tccgcaccgc gaatcggcat tgttaaggca tcatcagcat taaccacgca
    24001 aactttcgcg ttttcgtaaa tgcgcagttt tgctgcacga tactgttgta aaccaaacgg
    24061 atagcgatcc atatgatctt cagtcacgtt cagaatggtc gctgctaccg cctgtaagct
    24121 ggaggtggtt tccagctgga agctcgacag ttccagcacg tacagttcac actcatcatc
    24181 cagtagcatc aacgcaggca ggccaatatt gccacccaca ccaacgttaa cccccgccgc
    24241 tttcgccatt tcacccacta gcgtggtgac cgtgcttttg ccgttagaac cggtaatcgc
    24301 cacaatcggt gcttgtgctt cgcgacagaa cagctcgata tcgccaacga tttcgattcc
    24361 ggcatcagcg gcagcgctta aggatggatg cgccagtgca ataccgggac tggcgacaat
    24421 cagatctgcc gccatcagcc attcatcatt cagactgccc gtgtggcgtt ctacggcttc
    24481 aggtaattta tccaggccag gcggtgtcat acgcgtatcc ataacgcgcg gcgtcacacc
    24541 gcgagcgagg aaaaagtcca cgcaggaaag cccggtgagg cccaggccga taatgacaac
    24601 atttttaccc tggtaatcag ccatgattaa cgtaccttca gcgttgccag accaatcagg
    24661 accagcatca gcgaaataat ccagaaacgc acaatgacgc gcggttccgg ccagcctttc
    24721 agttcatagt ggtgatgaat cggtgccatg cggaaaatac gttgtccgcg cagtttaaag
    24781 gagccgacct gcaggatgac agaaagcgtt tctaccacga acacgccccc cataatcacc
    24841 agcaggaatt cctgacgtag cagtacggcg ataatgccta acgcaccacc taacgccagc
    24901 gaaccgacat cgcccataaa gacctgtgcc ggataggtgt taaaccacag gaagcccagt
    24961 cctgccccga ctatcgcggt acagacaata accagttccc cggcgtgtcg cagatacggt
    25021 atgtgtaaat agctggcaaa gttcatgttg ccggtcgccc acgccaccag cgcaaaacca
    25081 ccggcgacaa atacggtcgg cataattgcc agaccgtcaa gaccatcggt caggtttacc
    25141 gcgttgccag tacccacaat gacgaagtaa gccagcagaa tgtagaacag ccccagttgc
    25201 ggcatcacat ctttaaagaa tgggaccacc agctgcgttg cgggcgtgtc tttgccaaca
    25261 aggtacagcg cgaaggcgac acccagcgca atgaccgaca tccagaaata cttccaacga
    25321 gcgatcaacc ctttggtgtc tttacgcact actttgcgat aatcatcaac aaagccaata
    25381 acaccgtaac ctaccagcac caccaacacg caccagacgt acggattgga cgggtaagcc
    25441 cacagcagta cggagatcac aatcgccgtc aggatcataa tcccgcccat ggtcggcgta
    25501 ccgcgcttgc tgaagtgtga ttcaggaccg tcgttacgca ccacctgacc aaaggaaagt
    25561 ttttgcaaat gagcaatcat acgcgggccc atccacaatg agatgaacag cgcggtcagc
    25621 aggctgacga tggcgcgaaa cgtcagatag gaaaagacgt taaagccgga ataatatttg
    25681 accaaatgtt cggccagcca aactaacatg tcccattctc ctgtaatgcg cgtactacct
    25741 cttccatggc ggcactacgt gaacccttaa ctaaaatcgt aattacctgt tgctcagcaa
    25801 tcagtgattt aagacgcgta attaacgcag ttttatcagc aaaatgttcg ccaacgccgc
    25861 tggcagtgct gatagcatgg ctttgtttac ccacgcttaa cacgcggtca ataccagcag
    25921 cttttgccgc ttcgcccact tgtacatggc aggcttcgct ttcagcgccc agttccgcca
    25981 tatcgcccac caccagcacg cggtagcccg gcatttcagc cagtacctgg actgctgcgg
    26041 tcattgaacc gacattggcg ttgtaggagt cgtcgagcag caactggttt tctgctagtt
    26101 ggatggggaa cagacggcct ggaacagctt tcagatttgc cagccccgct ttgatagcat
    26161 caagcgttgc gcccacggac atggagagcg cagcggctgc cagcgcattc gcaatattgt
    26221 gacgccccgg caacggcagc agaacatcca cgctaccggt tggggtttgc agggtaaatt
    26281 ccgtaccgtg cgaagtcaca tggatattgg tggcggtgaa atcgctgttg gcggcattcg
    26341 gtgagaaacg ccacactttg cgtgagccaa ttacgctctg ccagttcagc cagtcgttgt
    26401 tgtcggcgtt cataatggcg ataccgtttt ccggcaggcc gctaaagatt tcacctttcg
    26461 ctttcgcgac ccccgcaagc gagccaaaac cttccagatg tgccgctgcc aggttgttga
    26521 ccagcgccgc ttccgggcga gtcagactca cagtccaggc tatttcgccc tgatggttcg
    26581 cgccaagttc aataactgcg taatcgtatt ccggcgttaa gcgcaacagc gtcatcggta
    26641 caccgatgtc gttgttgaga ttgcctgccg tataaagcgt gttgccgcac tggcttaaaa
    26701 tcgccgccgt catctcttta acggaggttt tgccggagga acctgtcaga gcaaccacgc
    26761 gcgccggaac ttgctggcga acccatgcag ccagttcacc aaacgccaga cgcgtatcct
    26821 tgacgattaa ctgcggcagg tcgatgtcca gcggacggct aaccagtagt gcgcctgcgc
    26881 cgccagcttt cgcctggtcg gcaaaatcat gagcatcgaa acgttcgcct ttcagggcaa
    26941 caaacaggca gcccggcgtc agttttcggg tatcagtggt tacagcatca agggtgatat
    27001 ctgcaccttg cagttcaccg ttgagaatgt cggtaagttg gctaagggtt acgctaatca
    27061 tgcaatcacc cccagcagac gcgccactgt gacgcgatcg gagtagtcca gacgctgatt
    27121 gccaacaatc tggtaatctt catggccttt gcccgcgacc agcaccacat cgttctcttt
    27181 agcctgcata acggcgcaag tcaccgcttc agcacggcct tccatcactt tggcatgtcc
    27241 ggcatctaac attcccgcca gaatatcgtt gatgatggca cgcggctctt cggtacgcgg
    27301 gttatcgtcc gtcaccaccg ccacgtcagc aaactcttcg gcaattgcgc ccatcagtgg
    27361 acgcttacct ttatcgcgat cgccaccaca gccaaagaca caccacagct tgcccgcaca
    27421 gtgcagacgc gccgcctgta aggctttttc cagtgcatcc ggcgtatgcg cgtaatccac
    27481 caccaccgtc ggtttgcctg gcgcagtgaa cacttccata cgtccgcaaa ccggttgcag
    27541 acgcgcggcg gttttcaaca gatcagccaa tggatagccg agtgccaaca gtgtcgccag
    27601 cgcgagcagc aggttgctga cgttaaaagc gcccatcaga tggctttcaa tttcgccatc
    27661 gccccaactt gagctaaagc gaatcgtcgc accgctgtcg tgatagttca cgtcgatcgc
    27721 ttttaaccag cgtccgtgac agttcggatt gatatgatct tccattgata ccgcaaccgc
    27781 gtccggcagt tttgccagcc agcggcggcc cacttcatcg tcggcgttaa taatcgcctg
    27841 accgcaatga tgttcagaat aaagcagcca tttcgcagct tcgtagtgtt ccatatcacc
    27901 atgataatca aggtgatcgc ggcttaagtt ggtaaagacc gacgccgcaa atttcaatgc
    27961 cgccacacgg tgctgtacca gcccgtggga ggaaacttcc attgcgcaaa aagtcgcgcc
    28021 ctgatccacc agccccgcca gctcatgctg aacatcgact gcggaaccgg tagtattttc
    28081 tgtcgggatc actttcccaa gcagaccgtt accaacggtg cccattaccg cgctggtttc
    28141 gccaagcagt tggctccact gcgccagcag ctgggtagtc gtggttttgc cgttggtgcc
    28201 cgttacgcct acgagacgta aattgtcaga gggttcatgg taaaagcggc ccgccagtgc
    28261 agataaacgc tcattgagct ggctgagata gatgaccggt acgccgtgca tttcacggat
    28321 ttcaccgtcg gtcgcctcat ctttcgcctc tgcaataatg gcagccacac cttgcgctat
    28381 cgcctgcggg atatatcgac gcccgtccgc ctgatgacct actacagcta caaagagatc
    28441 gcccgccgca gccacacggc tgtcgagtgt catctctcgc agtgctcgcg aaggtgcgtc
    28501 tggcacccac ggagcaagaa ggtcgcgcaa attacgatct gccacctgtc ccctcgcctt
    28561 gattaatcac aaattcattt ttatcgcccg ttgtcagcgc atccggctcg atgttcatgg
    28621 tacgcaatac gccgcccatg atggcaccaa agaccggcgc ggaaacggcg ccgccgtagt
    28681 atttacccgc ctgcggatcg ttgataacaa caaccagcgc gaagcgcggc tgactcgcag
    28741 gcgcaacgcc tgcggtataa gcaatatatt tattgatgta gcgaccgtcc ggcccgacct
    28801 ttttcgcggt accggtttta atggcgatac gatagccttt aatcgccgcc ttcacgccgc
    28861 cgccgcccgg tagcgccacg ctttccatca tatgcaccac ggtgcggaca atggattccg
    28921 ggaagatacg ttcaccggga accggggggt caactttggt aatcgacagt gggcgataaa
    28981 tgccgtagct gccgatagtt gcgtagactc gcgctaactg taacggtgtt accattagcc
    29041 cgtagccgaa agagaaggtg gccctctcta tgtcagacca ccgttgtttt tgaggatata
    29101 agccactgcg ttctccgacc aaccccaaat tggtcgcttt tcccagtcca aaacgtgagt
    29161 aagtatctac taacgctgag gacggcatcg ctaacgccag cttggaaaca ccgacgttac
    29221 tcgacttctg taataccccg gtcagggtta attcgctgta gcgtgccacg tctttgattt
    29281 cgtggccgtt aattcgataa ggaacggtat tgagtaccga gttttcccgc accacgccac
    29341 gttgcaacgc ggtcatcacc accatcggtt taaccgttga gcccggttca aacacgtcgg
    29401 tgatggtacg gttacgcatc gcctctttcg gcgtgccact cagattgtta gggttgtatg
    29461 atgggctgtt agccatcgcc agcacttcac cggtgttgac atccaccagc acggcgctac
    29521 cagattcagc cttgttaaag gccaccgcat tgttcagttc gcgataaacc agcgcctgca
    29581 ggcgttcatc aatactcagc gccaggttgt gcgcagcctg gctgtcagta gaagaaatat
    29641 cttcaattac gcgaccatag cggtctttac gcacaatgcg ctcacccggc tgcccggtaa
    29701 gccatttatc gaaactcttc tcaacgccct caatcccttg actatcgacg ttagtaaagc
    29761 caatgaggtg agcagtcact tcgccggatg gataataacg gcgagattct tcacgcagat
    29821 gaatccccgg cagtttcagt tttttgatgt agtccgccat gtcagggttt acctgacgcg
    29881 ccagataaat aaagcgccct ttcgggttgg cgttaatgcg ggcagaaagc tgatccagcg
    29941 gaatattgag cgcgttagcc agcgccttcc agcggtcacc gacgctgata ccgccagcgt
    30001 catgcacttc tttcgggtca gcccaaatcg cttttaccgg cacgctcacc gctaacgggc
    30061 gaccagaacg atcagtaatc atgccgcggg aggtggaaac ttgctgaacg cgaagagaac
    30121 gcatgtcgcc ctctttcacc agcatatccg gggagataac ttgtaaccac gctacgcgtc
    30181 cgagcagaaa agccagcgcc aggagaatac agccgcataa caacacaaaa cgccaactga
    30241 taaagttggc atgttcttcc tgacgttttg gtttctgcgt tttcgccgct gctttcatgc
    30301 gtcgcgttta tccttatttt tgcactacga tattttcttg tgacggatca acatgctgca
    30361 tttgcagctt ttccgtggcg atcctttcca cccggctatg gtcgccgagc gcattctctt
    30421 caaggatcag gttgcgccat tcaatgtcta aagcatctcg ctccagcacc agttgttcgc
    30481 gctgagcggt cagtaaacgg gtatggtgcg ccgtggttac cacggtcacc gccgtcaaaa
    30541 taatgcaaat gaacaggcag agtggcagct tcccaaatcg caaaagatcg tcaccgataa
    30601 caccaggcaa tgcatggcgc tcgtggcttc ccatcgatcc tttaactttg cttagagctt
    30661 ctgtcactct gctgatcatg cgttcgtcct ctctgcaata cgcagaactg aactacgggc
    30721 acgagggttc tcagccacct cttcttcgcc cggcattaac ttgcctagtg ctcgcagctg
    30781 acggccaccc agttttttga gctgctcttc agtcatcggt aaccctgccg gaacttgcgg
    30841 accgcggctg ttttcacgca taaaacgttt cacaatacgg tcttccagcg agtggaagct
    30901 gatgatcgaa agccgcccac ccggggccag cacgttgagc gagcttttta gcgcctgctc
    30961 tatctcctcc agttcactgt ttacccaaat gcgcaccgcc tggaaggtac gggtcgcggg
    31021 atgtttaaac ttatctttca ccggcgttgc agcagccacg acttccgcca gttctttggt
    31081 gcgggtcatc ggctgttcgc ggttacgctc gacaatggcg cgggcaatgc gtttggcaaa
    31141 acgctcttca ccataggttt tcaataccca ggcgatatcg gcttcttctg cggtttgtag
    31201 ccattcagcg gctgactgcc cacgggttgg gtccatacgc atgtccagcg gaccatcgcg
    31261 cataaaggaa aagccgcgtt cagcatcatc aagttgcggt gaagagacac caagatcgag
    31321 gagaatgccg tcgatcttgc cgataagatc gcgctcggca acgtattcgc ccagcgcgga
    31381 aaaaggtccg tggatgatgg agaagcgcgg atcatcaata gtcttcgcaa cggcgatagc
    31441 ctgcgggtcg cgatcgatcg ccagcaaacg cccctcttcg ccaagctgcg agaggatcag
    31501 acgtgagtga ccaccgcgac caaaagtccc atcaatgtag atgccatcag gacggatatt
    31561 gaggccatta acggcttcat ccagcagcac cgtagtatgt ttatagtttt ccatcatttt
    31621 atagagacaa gtcctgcagt cgctccgata agtctccggt agccaactgc tctgcgtcga
    31681 tatcttcctt gacctgttga tgccaggttg tttcatccca cagctcaaac ttgttgaact
    31741 gtccaaccag catcacttct tttgtcagcc cggcatgttg ccgcagtact ggcgcgatta
    31801 acaatcgacc tgcgccatcc atctgacatt cgctggcatg acctaacagt aggcgctgca
    31861 cacggcgctc aaccgggttc atgctcgaca gacgcgataa tttttgctcg ataatttccc
    31921 attcaggcag ggggtaaagc agcaggcacg ggtgatgaat gtcaatggtg caaatcattt
    31981 gaccggcagc gttctcaagc agctgttccc gataacgggt aggcactgat aagcgccctt
    32041 tgctgtcgag attgactaac gttgctcccc ggaacatgcc agcctccccc cttattccca
    32101 ctttgcccca caatttccca ctgaaaggag tttacggagc ggaggaaaag cttgtcaagc
    32161 cagtcacaag gcataccgag gtaaaaaacc cgatgtttac ggctaatatc agcgttgttt
    32221 attttacgtc caacgtgcga agcaaattaa cgttatgaat agctgtaaga aaaaaaacct
    32281 catcgtcatc accgttttaa aatgactcac tattttccac agcaaatata aagtgtcagt
    32341 ttgcgacgcg agcggcattt taggacatat cttccccggt taacagtccc tgttgcgtct
    32401 gtgtggcgct tgatgccagc gggtttgcgc caaaacaaag aaaagtgttt gttaaatcgg
    32461 caattcatcg ccgcatgtaa caaaataatg caaaaacgcg ctgtcgatat cacttaatta
    32521 ttgtgaattt tagctggtga gttaattaac gggcgggttt aaaagcattt atcaataata
    32581 gtcaggaata gtcttattta ctttaaggat attgatgtcc agtcccgtac tctacgcgcc
    32641 agagggaaat tcacctggcg cgtatttttg ttcgcggctt agctacggct gagcacaccg
    32701 cggcgataga gattacgttt aatgcgcgtt aaaccaggtt ttggcttacg cggttcgtcc
    32761 aggcttgcca ggacaatctc cagcacacgc tctgcgacat cgcggtgacg ttgagccact
    32821 gccagcaccg ggcactgtaa gaagtcgagc agttcgttat cgccaaaggt ggcaattgcc
    32881 aggtcagaag gcagtttacc atcgcgacgt agcgtgacat ccatcacccc ttgcaacaac
    32941 gcaaacgacg ttgtgaacag cgcctgcggc atcggatgcg tttccagcca tttttcgaat
    33001 aactgggcag ccgcctcccg ctcatagctg ttggcataca ggaaatgcac ttcgcgcgga
    33061 tcatctttcc aggcagtacg gaaaccttgt tcacgcagga agctgacaga aagctccggc
    33121 agcgcgccaa gataaagcac cgtctcggcg ggaaacttac gtaactcttc cgccagcatt
    33181 tcggcatcat cctgatcggc accaaccacg ctggtgaaat gttcacgatc gagggcgcgg
    33241 tccagcgcga caatcgggaa cgggtcgtta gcccagcgtt gataaaaagg atgctcaggt
    33301 ggcaacgacg tcgaaacaat aatggcatca acctgacgct gtaaaaggtg ctcaatgcag
    33361 cgcatttcgt tgtctggctg atcttctgag caggcaatca gcagttgata accccgttgc
    33421 cgcgcctggc gttcaagata gttagcgatg cgggtatagc tggtgttctc cagatcgggg
    33481 atcacaagac caatagaacg tgtgcgtcca gcacgaagcc cagctgccac ggcgttcggg
    33541 tggtaattgt gctcacgcac cacagccatg actttttcaa cggttttgtc gctcacacgg
    33601 tattgcttcg ctttgccgtt aataacatag cttgcagtgg tccgcgacac tcccgccagc
    33661 cgagcgattt catccagttt cacaattgcc ccttgcgtaa aatgtaaaaa ccatagccat
    33721 tgtactggta tgggttaaat tcattaacat ctaagcgcag aaaattaact gcggcaactg
    33781 cttttattcc aggttcccac tgatttcgca aaaaaagccc aacgtgacat gttgggctgc
    33841 aaattgcgca ctgagatcat tctcaacgca ttattttatc gccgcgcgaa agtccgacca
    33901 caccagagcg agcaacttcc acaattttcg ccacatcgcg aatcgatgct aaaaatgcat
    33961 caagcttatc gctggtgcct gctaattgaa cggtataaag cgagggtgtg acatcgataa
    34021 tttgcccacg gaatatttcc gtattacgtt tcacttcgtc acgcccgtaa ccgctggcct
    34081 gaattttcac cagcatgatt tcccgctcaa catgcgcgcc ctgccccaac tcactcacgc
    34141 gcaagacatc gaccagcttg tgtaattgct tttcgatctg ctcaagtact ttttcatcgc
    34201 ccacggtctg gatggtcata cgcgataatg tcggatcgtc ggttggcgca acggtcaggc
    34261 tttcaatgtt gtagcctcgc tgggaaaaaa ggccaatcac gcgggataac gcgcctgatt
    34321 cattttcgag taagactgat aatatccggc gcataatcag gttctctccg ttttgcttaa
    34381 ccacatttca tccattccgc ccccgcgaat ctgcatcggg tagacgtgct cactgccatc
    34441 gacggtaaca tcaacaaaca ccaggcgatt attgcgcacc tgttccagcg cctcgctaag
    34501 tttgctttcc agctcatgcg gatgagagat ctggatcccg acgtgaccat aggcttccgc
    34561 cagacggacg aaatcgggta gtgattgcat ataagattgt gaatgacggc cggaatagat
    34621 catgtcctgc cactgtttca ccatccccag atagcggtta ttgagattca ccaccagtac
    34681 gggcaactcg tattgcaagg cggtagacag ttcctggatg ttcatctgaa tactgccgtc
    34741 gccagtgacg caaaccacgg tttcttctgg caacgccatt ttgacgccca gtgccgcagg
    34801 taaaccaaaa cccatcgtgc cgaggccgcc ggaattgatc cagcgacgcg gtttgtcgaa
    34861 tggataataa agcgcagcaa acatctggtg ctgcccgaca tcggacgtca cgtaaacgtc
    34921 cccattagtc aaccgccaaa gagtctcgat caccgcctgc ggtttaatct tttcactgtg
    34981 agtgtcatat ttcaggcact gacgagcgcg ccactgttca atctgctgcc accagtcgcg
    35041 gatctcatcc agcggttgat gggcggattc ttgcgacagg agttcaagca tttgttcgag
    35101 gacctggcga gcatccccca caatcgggat atcagcagtc acggttttag aaatggaagt
    35161 aggatcaata tcgatatgca gaacagtggc atttgggcag tactttgcca gattattcgt
    35221 cgttcggtca tcaaatcgta ccccgacggc gaaaatcaca tccgcgttat gcatcgtcat
    35281 attggcttcg taggtaccgt gcattcccag catgcccagt gcctgacgat gcgttgccgg
    35341 aaacgccccc agccccatca atgagcaaac aacgggcaga ttcaacgcct ccaccgtttc
    35401 tttcaactgc tgatggcagc ccgccgtgat tgccccaccg cctacgtaga caactggttt
    35461 ttttgccgct accagcgttt gcagagcacg cttaatttgc cctttatgcc cggtagtagt
    35521 gggattgtaa gaacgcatac tgaccgactc cggccagaca tagggtaatt tgttcgccgg
    35581 attaagaata tctttcggta aatcaacgac tactggtcca ggacgaccac ttgctgccag
    35641 ccagaaagcc tttttcagca cctgcggaat gtcttccgtt tgcttaacca gaaaactgtg
    35701 tttaaccacc ggtcgcgaaa tccccaccat gtcgcactcc tgaaaggcat cgtaacctat
    35761 caacgaggtc gctacctgcc cggaaaggac aactaatgga atggaatcca tataagcggt
    35821 ggcgatgccg gtaatcgcat tggtcgcccc tggacccgac gttaccagca cgacgccgac
    35881 ttccccggtc gcacgcgcca ggccatcggc catatgcacc gccgcctgct catgacgaac
    35941 taatacatga tcaataccac ccacggtatg caatgcatcg taaatatcaa ggactgcgcc
    36001 tccgggataa ccgaatactt gtttaacgcc ctgatcgata agcgatcgga cgaccatctc
    36061 ggctccagac aacatctcca tggcctgcct cactgtttga cggaaaaaat gtgtaaaaca
    36121 cattaaccgt ccgatcttgc ttaagcaatt ggcagccaga gacataaacg cagggtaata
    36181 agaataaaca ggaggaaagg tgaaaagaaa cgataaaatc ctccattacg aaggattaaa
    36241 ttgataataa atcggctgaa tcccacaact tacagcaatt tctctatttt tttaattaat
    36301 cctaaaatag agagcccaca cattcaggat aaaattctac attcaataaa aaatgcagaa
    36361 taaaccagac attcatgtct gacctattct gcattcagtt agcgtttgca aattgagact
    36421 aattgctctt ccatccactg atggccttta tcgcgcccgg cagcttcatg ccaggagaga
    36481 taacaggttc tgctgttttg ttttaacggc agcggtaata cctgtaattc taaggattca
    36541 gcgaactctt cagccaacca acgcggcgca atagcgacca gatgcgtttg cgacaccacg
    36601 ctaagtacgc tcatcattgc catgccctga tacgcgatac tggcttgctt atctaccgtg
    36661 tcataccaag gttgactaaa tgatgcgaaa cgatcaagcg aaaccgccgc atgttgttcg
    36721 ttataaacat catgtttcag taacgggccc ttaattgttg gatgattttt gctggctacc
    36781 agcaccattt catctttaaa taatggtacg ctggtaaatt caggacgatg gaagtcttca
    36841 taactaatca caaactccgt ttcctgataa cgcagctgat gttcagtgtt ctgatttaat
    36901 gaagacttga acataacatg tatatttggc gcaatctgct caatgtgatt ataaatctgc
    36961 gaggtcagaa tgctgtctaa cgggctgcaa acacaaagat gaaatacacg ttcactgctc
    37021 gcgggttcaa aacctgaacc aggcaattca ttttgtacta gttgcaatgc ctggcgaact
    37081 gaaccaaaaa gttgaaatgc gcgagcagtc ggttgaatac cacggccata acgaacaaaa
    37141 agctcgtcat taaacatcac cttcaggcgt gcaacagcgt tactgaccgc aggttgcgac
    37201 attcccagaa catgagcggc acgggtaatg ttttgctcct gcatcacggc atcgaaaacg
    37261 gttaataagt tgagatcgac catgcgtagc tgtggcttgc ttaactccgc cgtctctgga
    37321 tgatctgttt gtacctctgg catacttaac tccactgtca cacttaactc cctttccctt
    37381 attggaatgc agagaaataa tcctggagaa tatgatttat catgcatata aaataagaaa
    37441 aaggaaaatg agtaaaatta ggaaaacata aagatacggg ttcttataaa aacagaatcc
    37501 attgccgtgg aaagcggcat ttaccacagc ataataatcc ataatgtttt tgttgcgaaa
    37561 acaatctaat cataactacc gcga
//
LOCUS       DAZKIV010000026        34313 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZKIV010000026 DAZKIV010000000
VERSION     DAZKIV010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34313
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.026"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            341..919
                     /gene="mntP"
                     /locus_tag="ACHOT6_001388"
     CDS             341..919
                     /gene="mntP"
                     /locus_tag="ACHOT6_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFP9946631.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEDEDEE
                     PRRRHGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGR
                     FIGSIIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(916..1725)
                     /gene="rlmA"
                     /locus_tag="ACHOT6_001389"
     CDS             complement(916..1725)
                     /gene="rlmA"
                     /locus_tag="ACHOT6_001389"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFP9946632.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1891..2100)
                     /gene="cspE"
                     /locus_tag="ACHOT6_001390"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1891..2100)
                     /gene="cspE"
                     /locus_tag="ACHOT6_001390"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9946633.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(2113..2256)
                     /gene="yobF"
                     /locus_tag="ACHOT6_001391"
     CDS             complement(2113..2256)
                     /gene="yobF"
                     /locus_tag="ACHOT6_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFP9946634.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2925..3212)
                     /gene="yebO"
                     /locus_tag="ACHOT6_001392"
     CDS             complement(2925..3212)
                     /gene="yebO"
                     /locus_tag="ACHOT6_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFP9946635.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(3287..3430)
                     /gene="mgrB"
                     /locus_tag="ACHOT6_001393"
     CDS             complement(3287..3430)
                     /gene="mgrB"
                     /locus_tag="ACHOT6_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFP9946636.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3589..3828
                     /gene="yobH"
                     /locus_tag="ACHOT6_001394"
     CDS             3589..3828
                     /gene="yobH"
                     /locus_tag="ACHOT6_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFP9946637.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(3971..4762)
                     /gene="kdgR"
                     /locus_tag="ACHOT6_001395"
     CDS             complement(3971..4762)
                     /gene="kdgR"
                     /locus_tag="ACHOT6_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFP9946638.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4939..6312
                     /gene="yebQ"
                     /locus_tag="ACHOT6_001396"
     CDS             4939..6312
                     /gene="yebQ"
                     /locus_tag="ACHOT6_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946639.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTSWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(6358..7239)
                     /gene="htpX"
                     /locus_tag="ACHOT6_001397"
     CDS             complement(6358..7239)
                     /gene="htpX"
                     /locus_tag="ACHOT6_001397"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFP9946640.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7431..9479)
                     /gene="prc"
                     /locus_tag="ACHOT6_001398"
     CDS             complement(7431..9479)
                     /gene="prc"
                     /locus_tag="ACHOT6_001398"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFP9946641.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9499..10197)
                     /gene="proQ"
                     /locus_tag="ACHOT6_001399"
     CDS             complement(9499..10197)
                     /gene="proQ"
                     /locus_tag="ACHOT6_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFP9946642.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDGQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(10294..10791)
                     /gene="msrC"
                     /locus_tag="ACHOT6_001400"
     CDS             complement(10294..10791)
                     /gene="msrC"
                     /locus_tag="ACHOT6_001400"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFP9946643.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10921..12204
                     /gene="yebS"
                     /locus_tag="ACHOT6_001401"
     CDS             10921..12204
                     /gene="yebS"
                     /locus_tag="ACHOT6_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFP9946644.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            12173..14806
                     /gene="yebT"
                     /locus_tag="ACHOT6_001402"
     CDS             12173..14806
                     /gene="yebT"
                     /locus_tag="ACHOT6_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFP9946645.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSVSGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRKPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            14886..16325
                     /gene="rsmF"
                     /locus_tag="ACHOT6_001403"
     CDS             14886..16325
                     /gene="rsmF"
                     /locus_tag="ACHOT6_001403"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFP9946646.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALNGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            16443..16679
                     /gene="yebV"
                     /locus_tag="ACHOT6_001404"
     CDS             16443..16679
                     /gene="yebV"
                     /locus_tag="ACHOT6_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFP9946647.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16784..16975
                     /gene="yebW"
                     /locus_tag="ACHOT6_001405"
     CDS             16784..16975
                     /gene="yebW"
                     /locus_tag="ACHOT6_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFP9946648.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(16976..17632)
                     /gene="pphA"
                     /locus_tag="ACHOT6_001406"
     CDS             complement(16976..17632)
                     /gene="pphA"
                     /locus_tag="ACHOT6_001406"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFP9946649.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERPKGQGIKGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(18028..18369)
                     /gene="yebY"
                     /locus_tag="ACHOT6_001407"
     CDS             complement(18028..18369)
                     /gene="yebY"
                     /locus_tag="ACHOT6_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFP9946650.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(18382..19254)
                     /gene="copD"
                     /locus_tag="ACHOT6_001408"
     CDS             complement(18382..19254)
                     /gene="copD"
                     /locus_tag="ACHOT6_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFP9946651.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGVGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(19258..19632)
                     /gene="yobA"
                     /locus_tag="ACHOT6_001409"
     CDS             complement(19258..19632)
                     /gene="yobA"
                     /locus_tag="ACHOT6_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFP9946652.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            19771..20001
                     /gene="holE"
                     /locus_tag="ACHOT6_001410"
     CDS             19771..20001
                     /gene="holE"
                     /locus_tag="ACHOT6_001410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFP9946653.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            20103..20759
                     /gene="yobB"
                     /locus_tag="ACHOT6_001411"
     CDS             20103..20759
                     /gene="yobB"
                     /locus_tag="ACHOT6_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFP9946654.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            20783..21445
                     /gene="exoX"
                     /locus_tag="ACHOT6_001412"
     CDS             20783..21445
                     /gene="exoX"
                     /locus_tag="ACHOT6_001412"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFP9946655.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(21442..23500)
                     /gene="ptrB"
                     /locus_tag="ACHOT6_001413"
                     /pseudo
     CDS             complement(21442..23500)
                     /gene="ptrB"
                     /locus_tag="ACHOT6_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
     gene            complement(23709..24368)
                     /gene="yebE"
                     /locus_tag="ACHOT6_001414"
     CDS             complement(23709..24368)
                     /gene="yebE"
                     /locus_tag="ACHOT6_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9946656.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSTDQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEGQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(24695..25051)
                     /gene="yebF"
                     /locus_tag="ACHOT6_001415"
     CDS             complement(24695..25051)
                     /gene="yebF"
                     /locus_tag="ACHOT6_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFP9946657.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCEAGMAEYQRR"
     gene            complement(25118..25408)
                     /gene="yebG"
                     /locus_tag="ACHOT6_001416"
     CDS             complement(25118..25408)
                     /gene="yebG"
                     /locus_tag="ACHOT6_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFP9946658.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            25542..26720
                     /gene="purT"
                     /locus_tag="ACHOT6_001417"
     CDS             25542..26720
                     /gene="purT"
                     /locus_tag="ACHOT6_001417"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFP9946659.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(26776..27417)
                     /gene="kdgA"
                     /locus_tag="ACHOT6_001418"
                     /gene_synonym="eda"
     CDS             complement(26776..27417)
                     /gene="kdgA"
                     /locus_tag="ACHOT6_001418"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFP9946660.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(27454..29265)
                     /gene="edd"
                     /locus_tag="ACHOT6_001419"
     CDS             complement(27454..29265)
                     /gene="edd"
                     /locus_tag="ACHOT6_001419"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFP9946661.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(29500..30975)
                     /gene="zwf"
                     /locus_tag="ACHOT6_001420"
     CDS             complement(29500..30975)
                     /gene="zwf"
                     /locus_tag="ACHOT6_001420"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFP9946662.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            31312..32181
                     /gene="hexR"
                     /locus_tag="ACHOT6_001421"
                     /gene_synonym="yebK"
     CDS             31312..32181
                     /gene="hexR"
                     /locus_tag="ACHOT6_001421"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFP9946663.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            32309..33751
                     /gene="pyk"
                     /locus_tag="ACHOT6_001422"
     CDS             32309..33751
                     /gene="pyk"
                     /locus_tag="ACHOT6_001422"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFP9946664.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAARLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(33811..>34313)
                     /locus_tag="ACHOT6_001423"
     CDS             complement(33811..>34313)
                     /locus_tag="ACHOT6_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NGG1p interacting factor NIF3"
                     /protein_id="HFP9946665.1"
                     /translation="VIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDDMYHVYAI
                     EPDSAINITKRFAKNLKIYNLPGIQFYGDRARIVRTIGIGAGCFCDPIQYMEYQADYY
                     ITINDSIKTWVQTQYSKDSGLPMAVIGYSVAQEAGMRRLASYLDLHSGYPCIHFTGGC
                     DYDWIE"
BASE COUNT         8412 a         8742 c         8352 g         8807 t
ORIGIN      
        1 tattttaacg tttcattgat atataaatct aaatgtaaat cgttatcaat aaagcaatga
       61 aataatatat tccaacagtt gttttatatt ctcaaaatat gttaaggttg cgccctcatt
      121 tggggagtag ccgatttcca gattccggaa atgtacgtgt caacatactc gttgcaaaac
      181 gtggcacgta cggactgaat actttcagtc aggcgagacc atatgcacat caatcgctat
      241 gcctgcatga acgcgaaaac catcgcggct tgttgttcat gtatgcatgt ttgctggggg
      301 cgatgatgtg ttttatggat accccggtca ggacattgtc atgaatatca ctgctactgt
      361 tcttcttgcg tttggtatgt cgatggatgc atttgctgca tcaatcggta aaggtgccac
      421 cctccataaa ccgaaatttt ctgaagcatt gcgaaccggc cttatttttg gtgccgtcga
      481 aaccctgacg ccgctgatcg gctggggaat gggcatgtta gccagccggt ttgtccttga
      541 atggaatcac tggattgcgt ttgtgctgct gatattcctt ggcgggcgaa tgattattga
      601 gggttttcgt ggcgcagatg atgaagatga agatgaagat gaagagccgc gccgtcggca
      661 cggtttctgg ctactggtaa ccaccgcgat tgccaccagc ctggatgcca tggctgtggg
      721 tgttggtctt gctttcctgc aggtcaacat tatcgcgacc gcattggcca ttggttgtgc
      781 aaccttgatt atgtcgacat tagggatgat ggttggtcgc tttatcggct caattattgg
      841 gaaaaaagcg gaaattctcg gcgggctggt gctgatcggc atcggcgtcc agatcctctg
      901 gacgcacttc cacggttaat aagaacgctg ccagaggtga atattaaagt ccgtctggca
      961 gtcgaacact tcttttgctg ccagtgtttg ccagacttct ggcttcgcac gccaggcaaa
     1021 cggcgtcatc tgcaataatg cgacggcttc atcaccgcga agacgcatcg gataacacaa
     1081 ctcatcactc tgctgtaatg taaaaccttc cagttgttct gcatgaggtg catgaagatg
     1141 tacttcattg taaatcagcc ccttcagctc catcaaatgt cgcggtcccg gcgtggcagt
     1201 aatgacccag ccgccgggct tcactactcg tgctaattct tctgctttac acggcgcgta
     1261 aatacgtatt atggcgtcca tactggtatc ggaaaacggc aaacggtggc tggaagcgac
     1321 acaaagagtg acctgcggat agcgtttcgc cgccgctttt atcgctacct tcgagacatc
     1381 caggccaaac gtggtgattt cgggcaacgc atcggcaaat gcgtgtgtgt aatacccttc
     1441 accacagcca atatccagca ccgccgtggc cttttcatca agccgttccc gcagttgggc
     1501 gacaattgca tcacgcagcg gctgataatg tccggcatct aagaatgcgc ggcgtgcttg
     1561 catcatttcc gcgctgtcgc ccggatcacg agaccgttta tgctgaacgg gcagcagatt
     1621 gacataccct tctttcgcca tatcaaactg atgtcgctgg ggacagatat agctgttttt
     1681 ttcacgcgaa agaggctgat ggcaaagtgg acaggaaaac gacatgacaa ctccggcagg
     1741 atactaaagg ccgcaagtgt aacgcgaatc acgcggcggg caaatatccg aacgccagga
     1801 gcgaagataa agcatagtaa aaagcctcgc attcgacgag gctttatagc gagattgaag
     1861 cgtattcaca cttcagatca gtggattcga tcagatagct gttacgttaa cagctgccgg
     1921 acctttctgg ccgtcctgaa tttcgaactc aacgttctga ccttcagcca gagttttgaa
     1981 gccattaccc tggatagcgg agaagtgtac gaacacatct ttgctgccat cagccggagt
     2041 aatgaagcca aaacctttag actcgttgaa ccacttaacc tgacctttaa tctttgccat
     2101 ttgaaaaatt ccttagattg ttttcttcgc ccgcaggcat aacatagata aaactgacac
     2161 attactgcat gaggcaccaa tataaggctc ggcagagaag cggtattcaa cgtcaacgtg
     2221 tttactcagg acttctttac tgaaaatgcc acacataaac agaactgtac ctcgtttaac
     2281 ccgaaatctg ttatcacata caacgttaat tatggcaagc catttttaaa catgtctcga
     2341 tcagacgcac aaatctcgat acgtcttcca ctttttttgc acacttatgc aacggaatac
     2401 gcgccgatat atcattgtgc ttaaccttgc cagttcaggc agatacttaa actggcgtat
     2461 tttctaacat agttcaataa gttcagttgt tccaggccag tgggagaagt tattacatag
     2521 tgcgtgcaat atcacatttt ttgctatgca atgaataaaa agttatatca ctttttctca
     2581 taaaacagtc agttaacggc tatcaattac cctaaagaga agtcaatccc caaagggatt
     2641 gaaaatttaa aataagaaaa attgatgaat gagcaaaaaa tcaagagaga aacgtttcgc
     2701 taataaataa taccacgctt aatcacaaat ccgatgtaaa catcctaata aattaatgag
     2761 gtaaatatct ttttctgata tcccgttacg caaataaata atatgataat tatttgaagt
     2821 cagaccagag ttttaatttg aagcagttgc accatggcag tgcgtataat caggatgaat
     2881 tgagtaaccc ttcaacgacg gggttactca agattgagga aggattatcg ttcggcgacc
     2941 agacgaataa tatcttcgtc ttcaaccgat ttttgactct caacggtctt tttatctgct
     3001 ttctcaggtt cgtttgcttc gcacaaacga cgtaacagtg cattttgacg tttttgctga
     3061 tccagcaacg cctcaagcag ttcaatctgt tcgttagtcc gagaactggc acgattgata
     3121 aaaaaccaca agatgagccc gataagaaga accaccaccg atacaaccaa agacgcaatg
     3181 ttcatcacgc ctgaatttac aacttcgttc atttcaccac ctctgagtag aggcgctatt
     3241 ctaccactgc tggagaggaa gaaaatctag tgctgaaaaa atgatatcac cacgggataa
     3301 actggttaat ggcacaaatt ccgctgaaaa attgtacatc ctgatcgcac atcatgttga
     3361 atacctgcgc ccaaagcagc aagcaagcca acaccacgac aaccagaacg acccatcgaa
     3421 actttttcac tccaatctcc gcttcaccta ccttatgtca ttaaactagc atgcgttagt
     3481 taaacagcgc ctaactatgt cgatattcgt gctttttcgg aatgagtagc ttgcttcagc
     3541 gcatattgcc gctacgatta agcgaacata aaaagagaag aggttgtaat gcgattcatc
     3601 attcgcacag ttatgctgat agcactggta tggataggat tattactcag cggatatggc
     3661 gttttgattg gaagcaaaga gaatgccgca ggattaggtt tgcaatgtac ctatctgact
     3721 gccagaggca ccagcacggt acaatacttg cacactaaaa gtggtttctt ggggataaca
     3781 gattgcccac tgttacgtaa aagcaatatc gtggtcgata atggttaagg gtaataaata
     3841 accagattct cactcatgag taaaatattc ttatagcaag cgcgtttaat ataacgcacc
     3901 ttcaacttta tttttctcat gctaaataaa gaacctgtct gtttgatatt ccgacaggtt
     3961 cttactctca tcagaacgga tagtcgtgat aacccatttg ggcagaaatt ttgcgcgctg
     4021 cggtatgcaa cattgcgaca tattcctgta aacgctcttc agagaaacgc aacgtcggga
     4081 aggagatgct caaaccggca atgaccacgc caaagcgatc aaataccggt accgcaatgc
     4141 atcgcagccc ttcttcctgc tcttcgttat cttcgccata cccctgctcg cgcacctggt
     4201 ccagaacggg taataacgct tctgtactgg tgatggtccg ctcggtactg cgtttatact
     4261 ccacgccctc aagaatttgc ttcacttcat cgcgatcgcg ccatgccagc agtaccttac
     4321 caatcgcggt gctgtacagc ggattacgac gcccaatccg tgaatacatg cgcaaattgt
     4381 acatagagtc aattttgtga atgtaaacaa tactgtcttc gtccagtgcg ccgaggtgga
     4441 tagtttcttt ggtcaggcgg gagatctcac gcatctggat atctgcgcta cgaattaaat
     4501 cgacgttttg taacgcgcga gcgcccagtt caaacaattt cagggtcagg gaatatttct
     4561 ccgactcccc ttcctgcgcc acataaccta aggttttcat ggtctgtaaa aagcgataaa
     4621 cggtgctttt tgacatcatg acgcgctgcg acagctcggt tatccctatt tcgcgctctt
     4681 cacccagcgc ctgcaaaatg ccaaaaactt ttagcacgga agatacagaa tcaggctgtt
     4741 tatccagatc tgcgttagcc atttatcacc tcattgcgag tgttttataa aaatcagaac
     4801 tgttttttat tataatttcg caccagggtg gtcgcaatcc atcttttgcc ggttagttac
     4861 aattctgcga catccaccgt gaatatcagt gctagaatca tacccctgtt gattattcac
     4921 caaagatata aaattcctat gccaaaagtt caggccgacg gcctgccatt gccccagcga
     4981 tacggtgcga tattaaccat tgtgattggc atttcgatgg ctgtccttga cggcgcaatc
     5041 gccaacgtcg ccctgccaac aatcgccacg gaccttcatg ccacgccagc cagttccatc
     5101 tgggtagtga acgcctatca aatcgccatt gtcatctccc tgctctcgtt ttcgtttctg
     5161 ggcgatatgt ttggctatcg acgtatttat aaatgcggtc tggtcgtttt tctgttgtct
     5221 tcactgttct gcgccctttc tgattcgctg caaatgctca cccttgcgcg tgtcatacaa
     5281 ggtttcggcg gtgcagcgtt gatgagcgtt aataccgcac ttatccgcct gatctatcca
     5341 caacgttttc tggggagagg gatgggcata aactcgttta ttgttgccgt ctcttctgct
     5401 gccgggccga caattgctgc agcaatcctc tccatcgcat cctggaaatg gttattttta
     5461 atcaacgtac cgttaggtat tatcgccctg cttctggcga tgcgttttct gccacccaat
     5521 ggttctcgcg ccagtaaacc ccgtttcgac ctgcccagcg ccgtgatgaa cgcgttaacc
     5581 ttcggcctgc ttatcactgc gttgagtggt ttcgctcagg ggcaatcgct gacgttaatt
     5641 gctgcggaac tggtggtaat ggttgttgtt ggtattttct ttattcgccg ccagctttct
     5701 cttcccgtac cgctgctacc ggtggattta ctgcgtatcc cgctgttttc actttctatt
     5761 tgcacatctg tttgctcttt ctgcgcacaa atgctggcaa tggtttccct gcccttttac
     5821 ctgcaaaccg tgctcgggcg tagtgaagtc gaaacaggtt tacttctgac atcgtggccg
     5881 ttagcaacga tggtgatggc tccgctggca ggctatttga ttgaacgcgt acatgcagga
     5941 ttgctgggtg ctttagggtt gttcatcatg gctgcggggc ttttttccct ggttctgctg
     6001 cccgcgtcac ctgcggatat caatattatc tggccgatga tcttatgtgg cgctggattt
     6061 ggcttattcc agtcacccaa taatcacacc attattacct ccgctccgcg cgaacgtagc
     6121 ggtggggcca gtggcatgtt aggaacggct cgtctactgg gtcagagtag tggcgcggcg
     6181 ctggtggcgc tgatgctaaa tcagttcggt gataatggta cgcacgtttc gctgatggct
     6241 gcggctattc tggcagtgat tgctgcctgt gtgagtggtt tacgtatcac tcagccacga
     6301 tccagggcat aaaaaaagcg cgtcgatcag gacgcgcttt ttagtattta cttcatatta
     6361 cttcaggtat tcacccgtac gcagagcttc aattcgttta tccagcggcg ggtgagtcat
     6421 gaacaactca ctgagcgatt tcgacttacc gttaatgcag aaagccatca tgctggttgc
     6481 ttcttgcggt tcatagctgg ttttcaggcg ctgcagcgcg gcaatcattt tctcgcgacc
     6541 aaccagtttt gccgaaccgg catcagcatg gaattcacga tgacgcgaga accacatggt
     6601 gataatgctc gccagaatac caaacaccag ttccagaacc gttgcaaccg caaagtagat
     6661 cagcgggttg ccgttgctct cttcaccttc atcacgattt ccgcccataa aacccgcggc
     6721 aagctgcgcc agaatacggg aaataaagat aacgaaggtg ttcaccacgc cctgaatcag
     6781 cgtcatggta accatatcac cattggcgat gtggctgatt tcgtgagcaa ttaccgcctc
     6841 ggcttcatcc gggctcatgt tctgcagcaa accggtgctg acagcaacca gagaggcatc
     6901 acggcgcgca ccggttgcaa aagcgttgat gtccggcgca tggtagatag ccacttgcgg
     6961 catagcgatc cctgcctgac gagcctgggt tgctacagta ttaaccagcc aacgttccct
     7021 ttcgttacgc ggttgctcga tcacttcccc gccaacagat cgtaatgcca tccatttgga
     7081 catcagaagc gaaacgaagg aaccaccaaa accgaacagc aaggccatga tcatcagccc
     7141 ctgaacgctg ctcgactgta tccctgtcag gctcagtacc agcccgaaaa cgaccattac
     7201 ggccaggttc gttagcagga agagcgcgat tcgcatcata attttctttt tacctcagtt
     7261 taacaaaacg caatatgcga tacccacatc gtatgggtta cgcgactatt ttcaagtctg
     7321 gatagtgcgt aagtcaccag aaagacacaa ctttacattt tgtagcatct gatttacggc
     7381 atcttgccgc tgttaaaaaa tcaggcacaa tttcttgtgc ctgattgata ttacttgacg
     7441 ggagcgggtt gttccgcggg tctggctttt tcaagcttcg ccagatcgag tgcgatattc
     7501 accgtctcat ccagataagg atccggctcc tggtaatctt ttggtagatc atccagcttc
     7561 ttcaactccg gtttaccttc gcgtttaaag cgttcgttca aacgcgccag acgcgtcgca
     7621 tcatcttcat tattctcttt ctcacgcaca gcgtaattca gagaaacgat attgcgcttg
     7681 tccttcatag cgttgaagcg cgcgatatcc ttcatgatgt tctggaactc aggatctttc
     7741 gcgatacgcg cattatgttc cttcagtagc tccggttcaa aggccgttaa atcgcctgat
     7801 ttcacataag tcgcagcatc aatgctatcc cacggcagcg cgttatcttc gaatttctca
     7861 cccgtttccg tttcttcatt acccgtcggc atgatgatgt ctggcgttac gcctttacgt
     7921 tgcgtactgc cgccgttaac gcgatagaat ttctggatcg tgtactgcac agaacccagc
     7981 gctggccatt caggacgtaa catctgatcg taaatacggt tcaatgaacg gtattgctga
     8041 acggtgcctt taccaaacgt cggttcaccc acaaccagcg cacgaccgta atcctgcatt
     8101 gccgcggcaa agatttctga agccgaagca ctgaaacggt caaccagtac caccagcggg
     8161 cctttataga acacctgccc gtcggtatcg ctgtcttcac gaaccttgcc gttgttatcg
     8221 cggacctgaa caatgggacc cgcaggaata aacagacctg agagcgatac cgcttcggtc
     8281 aacgccccac cgccattgct acgcaggtcg atgatcacac tgctgacatt ctgtttttcc
     8341 agtttctgca gttgaacttt gacatcgtct gtcaaaccca catagaagcc cggaatatcc
     8401 agcacgccga ctttctcttt accgacggtc ttcaccgaca ttttaaccgc gcggtcttcg
     8461 agacgaatac gttcacgggt caatgttaca gtgcgggtct tggtcccttt accagcaggt
     8521 aaaatttcca gacgaacttt actgcccttc ggccctttaa ttaaggcaac cacatcatca
     8581 agacgccagc caatcacgtc aaccatcggc ttgcctgttt gaccaacacc gacaattttg
     8641 tcaccaacgc tgatagcttt actcttcgct gccggaccac ctgccaccat cgaattgata
     8701 acggtgtagt catcatccat ttgcagcact gcgccaatac cttccagcga caagctcatt
     8761 tcagtgttga actgttcggt attacgcggg gaaagatagt tggtatgcgg gtcgatttca
     8821 cgcgcaaacg ccgtcattgc cagcgagaaa acatcttcgc tgttggtttg cgccagacga
     8881 cgaatggcaa atttgtagcg gcgtgtcagg gtttcacgaa tttctttatc cgtttttcct
     8941 gccagcttca ggcttaactc gtcgaatttg actttactgt cccacagcgc gttcaactca
     9001 gcctcgtttt tcggccaggg cgctttgctg cggtcaaggt tataagtgtc gttgccggtg
     9061 aaatccatcg gcttttccag taccgacaaa gcgtactggt aacgctcaaa acggcgcttt
     9121 tgcgccagat tgtagagatc gtagaaaacg tcgagtttgc ctgaacgcag ttcatcgcct
     9181 aactcggttt tctttttcgc gaactgttca acatcgcttg ccagcagcac gttgtggctg
     9241 taatcgagca gattcaggta gcggtcaaag attttggccg aaaatgcctg atcgaggtcg
     9301 aactggcgat aatgagaacg ggtgaagcgc gacgttacgc gctcacttac cgtcgcatgc
     9361 tgcgtctctt cctttaatac cggaatttga tcagcacgcg tgatatcttc tacagcgaag
     9421 gtctggcctg ctattgcaag caggccagct aacgcggtaa gcctaaaaaa catgttcatg
     9481 cctggcccgg cctccgtttc agaacaccag gtgttctgcg cgcacaatca aagacatacc
     9541 cgaattcagc tggacgcgga caccgtcttt ggtgatttct aatacggtgg catccatcgc
     9601 gttttgaccc gctttcacct tcagggcttg tccgacagtc agagctgaaa tgtcagaaac
     9661 cggggtgtgc tgttcttcgc gaggtgcttt tactgttttt ggcgctttct ctaccggctt
     9721 ttgcgcacga ggtttacgtt cagcgccttc tttgcggcgt ggcgtggtcg gacgtggctt
     9781 gcgttcgcgg cgcggtgcgt cttctttctc accagcagct gcggcagctt cgcgtttttt
     9841 cgcttgctgt tcagcacgct gtgcctgaac acgcgctttc gcttcttcaa gctgcttgcg
     9901 agcatgctct acatgttgcc cgtccagctc accgcatggg ttgccgtcaa gatcgacacg
     9961 cgttgcgccg ggtttaacac cgtaaagata acgccagctc gaagtgtaga gacgtaaagc
    10021 ggatcgtaat tgcgttttgc tcaggttcat ttccccggca acacgatcga ccaaatcctg
    10081 aaaaataccg attttcagcg gacgcgcttc accttccgca ctgaaacagt ggggaaaacg
    10141 ttcggccaga aacgcgatta cttctttact gctattcaac ttaggttgat tttccatgaa
    10201 atttcctgat tacaacggac gtagccaaca agcgcaggca tgaacaggcg tcattataat
    10261 gacgctacca gtaaatgcta cgttatccgt tgattatcct gcgacgctcg caaagaattt
    10321 tttgtaatcc gtcgttgcaa gcactttttc aagctgtgcc acaagctgac gtaagccttg
    10381 ctcgtcctcg tctgtaaagc gagcgaagac ggtactatcg atgtcaagaa caccaataat
    10441 ctgatttttc accaccagcg gcagaacaat ttcagaatta ctcgccgcat cacaggcaat
    10501 atgcccgtca aacgcgtgaa catcctcgat acgctgcact tgattgcggg caaccgcagt
    10561 gccgcacacc ccgcgcccga cgggtatccg gacacaggca attttgccct gaaatggtcc
    10621 gagtaccagt gtatcgtcct caagcaaata aaaacctgcc cagtttacgt cagtgagacg
    10681 ctcgtataac aacgcactgg tgttcgcaag cgttgccaga aaactggttt ctcccgccat
    10741 cagcgcgtta aagtcgcgat ttaaatccgc gtaaaattct gttttgttca ttatataatc
    10801 acttggttgt cttacctgga actgccagcc tattaaaata agcattaaat gcgttaatgc
    10861 tcaagatcat tcccaacatg ggttaatatt aatgttaatt cttattacat ttggcacgtc
    10921 atggctctta acacaccaca aattacgccg acaaaaaaga taacagtgag gtcaatcggc
    10981 gaggaactgc cgcgtggtga ttaccaacgt tgcccgcaat gcgacatgct gtttagcctg
    11041 cccgagataa attctcatca gagtgcctat tgtccgcgct gtcaggcaaa aattcgcgac
    11101 gggcgcgact ggtcgctaac gcgcctggcg gcaatggctt tcaccatgct gttgctgatg
    11161 ccgtttgcct ggggcgaacc gctgttgcat atctggctgt taggtattcg tatcgacgcc
    11221 aacgtcatgc aaggcatctg gcaaatgacc aaacagggcg atacgataac gggggcgatg
    11281 gtctttttct gcgttattgg tgcccccctc attctggtgt cctccatagc atatttatgg
    11341 tttggtaacc gactgggaat gaatttacgt ccggtactgc tgatgcttga gcgtcttaaa
    11401 gagtgggtaa tgctggatat ctacctggtc ggcattggcg ttgcttctat aaaggtacag
    11461 gattatgccc atatccaggc tggtgtcggc ttgttctctt ttgtggcgtt ggtgatttta
    11521 acgacggtga cgttgtcaca tcttaatgtt gaggagctgt gggagcgata ttatccgcag
    11581 cgccccgcta cgcgtaggga cgagaaactt cgtgtctgtc ttgggtgcca ttttaccggc
    11641 tatcctgatc agcgtggtcg ctgcccgcgt tgccatatcc cgctacgcct gcgtcgccgt
    11701 catagtctgc aaaaatgctg ggcggcgctg ttagcgtcaa tcgttttgtt gttaccagcc
    11761 aacctattgc ctatttctat catttatctg aacggaggac ggcaggaaga tacaattctt
    11821 tccggaatta tgtcgctggc aagtagcaac attgcggttg cgggaatcgt gtttatcgcc
    11881 agtattctgg taccgtttac taaagtgatc gtcatgttca ctttactgtt gagcattcat
    11941 tttaaatgcc agcaaggttt acgcacacgc attctgttac tgcggatggt gacctggatt
    12001 ggacgctggt cgatgctcga cctgtttgtc atatctttaa ccatgtcgct gattaatcgc
    12061 gatcagatcc tcgcttttac tatgggaccg gctgcgtttt attttggcgc agccgtaatt
    12121 ttgactattc ttgctgtgga atggctggac agccgcttac tttgggatgc acatgagtca
    12181 ggaaacgccc gcttcgacga ctgaagcgca gattaaaaat aaacgccgta tctcaccttt
    12241 ctggctgctg cctttcatcg cgctaatgat tgccggttgg ctgatttggg acagttatca
    12301 ggaccggggt aataccgtca ccatcgactt tatgtcggcg gatggtattg ttccgggccg
    12361 tacgcctgtt cgttatcagg gcgttgaagt cggaacagtg caggatatca gcctcagcga
    12421 cgatcttcgt aagattgaag tcaaggtcag catcaagtcc gatatgaaag atgcgctgcg
    12481 cgaagagact caattctggc tggtgacgcc aaaagcatcg ttggcaggtg tctccgggct
    12541 ggacgccctc gtcggtggga actatatcgg catgatgccg ggtaaaggta aagagcagga
    12601 tcactttgtc gcactcgata cccaaccgaa gtatcggctg gataacggcg atctgatgat
    12661 ccacctgcaa gctcccgatc tcggttcgct gaacagcggt tcattggtct atttccgcaa
    12721 gatcccggtg ggaaaagtct acgactatgc catcaatccc aacaagcaag gcgtggtgat
    12781 tgatgtcctg atcgagcggc gttttaccga tctggtgaaa aaaggtagcc gtttctggaa
    12841 cgtttccggc gttgatgcca acgtcagtgt cagtggcgcg aaggtgaaac tggaaagtct
    12901 ggcggcactg gttaacggtg cgattgcctt cgattcacct gaagagtcga aacctgccga
    12961 ggcggaagat acctttggtc tgtatgaaga tctggcccac agccagcgtg gcgtaataat
    13021 aaaactggaa ctgccgagtg gggccggatt gaccgccgac tcgacgccgt taatgtatca
    13081 ggggctggaa gtcggacagc taactaaact ggatttaaat cctggtggta aagtcactgg
    13141 ggaaatgaca gttgatccca gcgtcgttac cctgcttcgt gaaaataccc gcatcgaatt
    13201 acgtaagccg aaattatccc ttagcgatgc caatctcagc gccctgctga ctggcaaaac
    13261 cttcgagttg gtacccggcg atggcgagcc acgcaaagag ttcgttgttg tgccaggcga
    13321 aaaagcactg ctgcatgaac ctgatgttct gacgctgacc ctgaccgcgc cggaaagtta
    13381 cggtattgat gctggtcagc cgctcattct tcacggcgtg caggtaggcc aggttatcga
    13441 tcgtaaactc accagcaaag gcgtcacctt taccgtcgcc atcgagcctc agcatcgaga
    13501 actggtaaaa ggcgatagca aatttgtcgt caacagccgt gtcgacgtga aggtggggct
    13561 ggatggcgtt gagtttctcg gtgccagcgc ctcagaatgg attaacggcg ggatacgtat
    13621 tctgccgggc gataaaggcg agatgaaagc cagctatcca ctgtatgcca atctggaaaa
    13681 agcgctggag aacagcctta gcgatttacc caccacaacc gtgagtttga gtgcagagac
    13741 gctgccggat gtgcaggcag gatcggtagt gctgtaccgt aaatttgaag ttggtgaagt
    13801 tattaccgtg cgtccgcgag ctaacgcgtt tgatatcgat ctgcatatta agccggagta
    13861 tcgcaacctt ctgaccagca atagcgtgtt ctgggcagaa ggcggggcga aagttcagct
    13921 gaatggtagt ggcctgaccg tacaggcatc cccgctctcc agagcattaa aaggggctat
    13981 tagcttcgat aacctcagtg gtgccagcgc cagtcagcgt aaaggcgaca aacgtattct
    14041 gtatgcttcc gaaacagcgg cccgtgcggt tggtgggcag attacgcttc acgctttcga
    14101 tgccggaaaa ctggcggtcg ggatgccaat tcgctatctc ggtattgata tcgggcaaat
    14161 ccagacgctg gatctgatta ccgcgcgcaa tgaagtacag gcaaaggcgg tgctctatcc
    14221 ggaatatgtc cagacctttg ctcgcggtgg tacgcgcttc tcagtggtca caccgcaaat
    14281 ttcggcagct ggcgttgagc atcttgatac tatcctccag ccgtatatca acgtcgaacc
    14341 aggccggggc aatcctcgcc gcgactttga attacaagag gccaccatta ctgattcgcg
    14401 ttacctggat ggcttaagca ttattgttga agcgccggaa gccggttcgt taggtattgg
    14461 tacgcctgtg ctgttccgtg gtctggaagt cggtacggtt acaggaatga cgctggggac
    14521 attgtcagat cgcgtgatga ttgcgatgcg catcagtaaa cgctatcaac acctggtgcg
    14581 taacaattcc gtcttctggt tggcatcggg ttacagtctg gactttggtc tgacgggcgg
    14641 cgtagtgaaa accggcacct ttaaccagtt tatccgtggc ggcatcgcct tcgccacgcc
    14701 tccgggtacg ccactggcac cgaaagccca ggaaggcaaa cacttcctgt tgcaggaaag
    14761 tgaaccgaaa gagtggcgtg aatggggaac tgcgcttccc aaataatgcc cactgctccg
    14821 gcgtgcctgc gccggagcgt ttatgctaaa ctgcgcacct gtttttttgc cagtggtaca
    14881 tgctcgtggc ccaacacacc gtttatttcc cggacgcctt tctgacacaa atgcgcgaag
    14941 cgatgccttc gacgctctca tttgatgatt ttcttgccgc ctgtcagcgc ccgttgcgcc
    15001 gcagcattcg cgttaatacg ctgaaaatct ccgttgctga tttcctgcaa ttaaccgctc
    15061 cttatggctg gacgcttacg ccaattccgt ggtgtgaaga aggtttctgg attgaacgcg
    15121 acaatgaaga tgcattgcca ttgggtagta ccgccgagca tttaagcggc ctgttttata
    15181 ttcaggaagc cagttcaatg ttgcccgttg ccgccttgtt tgctgacgat aatgcaccac
    15241 agcgggtgat ggatgtcgca gctgcgccag gctccaaaac gacgcaaatt gccgcgcgga
    15301 tgaataacga aggggcaatc cttgccaatg agttttccgc cagtcgggta aaagtgttac
    15361 atgccaatat cagccgctgt ggcatcagta atgttgcgct cacacatttt gatggccgcg
    15421 tgtttggtgc ggcagtgcca gaaatgttcg atgccatttt gctggacgct ccctgctctg
    15481 gcgaaggcgt ggtgcgtaaa gatcccgatg cgctaaaaaa ctggtcacca gaaagcaatc
    15541 aggaaatcgc agctacacaa cgggagctta tcgacagcgc ctttcatgca ttacgtcctg
    15601 gtggtacgct ggtttactcg acctgtacct taaaccagga agaaaacgaa gccgtttgcc
    15661 tgtggctgaa agagacttac cccgacgcag tagagttttt accacttggc gatctcttcc
    15721 ctggtgcaaa caaagcgctg accgaagaag gctttttgca tgttttccca caaatttacg
    15781 actgcgaagg cttcttcgtt gctcgtctgc gtaaaactca ggcgatcccc gccttacccg
    15841 cccccaaata caaagtgggc aatttcccgt tcagcccggt gaaagatcgc gaagccggac
    15901 aaattcgtca ggcggctgca agtgttggct taaactggga tggaaacctg cgactctggc
    15961 aacgcgacaa agaactgtgg ttgttcccgg taggcattga agccctgaac ggtaaagtcc
    16021 gattttctcg gttggggatt aaacttgccg aaacgcataa caaaggttat cgctggcagc
    16081 atgaagcggt tattgccctt gcctcccccg acaatgtgaa cgcttttgaa ctgacaccgc
    16141 aggaagcgga agagtggtat cgcgggcgcg atgtttaccc gcaagccgcg ccagtagcgg
    16201 atgatgtatt ggttactttt cagcatcagc cgattggttt agccaaacga attggttctc
    16261 gactgaaaaa cagctacccg cgtgaactgg tgcgcgatgg gaaacttttt accggtaacg
    16321 cctgacagcg cacaaaaaaa gcgcactttt tgactggcac attcggctgc ctcaactagg
    16381 ctgaaaaatg gtgcgatcga actggtcgta ccacaaccgg cagctaaatg gagagcacga
    16441 agatgaaaac cagtgtgcgc ataggcgctt ttgaaatcga cgacggcgaa ttacacggtg
    16501 aatcgccggg tgatcgaacg ttaaccattc cttgtaaatc tgaccccgat ttatgtatgc
    16561 aactggatgc ctgggatgct gaaaccagta tccctgccct gctaaatggc gaacactctg
    16621 tcctttaccg tacccgttac gatcaacaat ctgatgcctg gattatgcgt cttgcctgat
    16681 ccaaaaagaa cccgtcggca tggcgggtta tttgtcctgg ttattccccc gttgtaaaat
    16741 ctctcctaaa cttaacggta cggcaccaca cttcggggat gaaatgttcg cgctggtact
    16801 ttttgtttgc tacctggatg gcggttgtga agatattgtt gtggatgtct acaacacgga
    16861 acagcagtgt ctttattcta tgagcgatca acggatccgc catggcggtt gttttccgat
    16921 tgaggatttt atagatggtt tctggcgacc agcacaggag tacggtgatt tttaattatt
    16981 gcagttgcac aagagtcagc tcgcccccaa agacagcgcc ggtatcaata taatgcaggt
    17041 tgccaatatc cacgcgatgt cgcaacggtg tatgaccaaa ccagaaatga tcagcacctt
    17101 taattccctg cccttttggg cgttcaccta atcgcgagcg gctccacaag acctgatgca
    17161 aatcaacgtc cttttgccat tcataaacat catctggata atcggcatga gcaataacat
    17221 gcttgccggt gcgactgtgt acttcaagaa taaagggcaa atgctgacat ttttccagcg
    17281 ccgttttcgc ttgtttctgt tgattatctg ccagcgcaat aaaccagtcg ccgccattca
    17341 tcaaccacaa agacatctgc tgggatgcca gcgcatccat cgccatctgt tcatgattgc
    17401 ctcttaccgc acaaacccaa tgttgttcca gtaactgcag acaacgtaaa ctttgcggcc
    17461 cacgatcgat aacgtctccc actgagataa gtaaatctcg ccacggatca aaacgacaat
    17521 gccataattt gcggcgcaac tgctcaagac aaccgtgtat atcgccagaa agccagatat
    17581 gtcgccattg atgacccgca attctctgat aaacgggcgc aggctgtttc atcaatattt
    17641 tcctcccgcg ctaaagatca cataatctta acaagaatgt taaaaaacgc tggactcaga
    17701 cagtagagtg tgtgttatgg ttgactataa agtcagcgaa ggaaatgctt ctggctttta
    17761 acagataaaa agagaccgaa cacgattcct gtattcggtc cagggaaatg gctcttggga
    17821 gagagccgtg cgctaaaagt tggcattaat gcaggcttag ttgccttgcc ctttaagaat
    17881 agatgacgac gccaggtttt ccagtttgcg tgcaaaatgg tcaataaaaa gcgtggtggt
    17941 catcagctga aatgttaaaa accgcccgtt ctggtgaaag aactgaggcg gtttttttat
    18001 tggaaatcaa aaggctattt taggtaatta acagagtttt tcagctcgtt ctataaacgg
    18061 tgccagactc attttttcgc cgggattgtt aggatcatca atctgaatca ccgaaatggg
    18121 ttgggcattg gtcttcccac tggcaacttc cttttgtgcg atatcgttta aaggatactg
    18181 cacgagggta ctcggattaa taacatacaa agcattaccc ggtcggcaag tcagcatcac
    18241 ctcttcgcga ttaaacgccc atttgtcttt acccacttca aaacgactga cggtaatcac
    18301 ctgcggtgca gccagcgccg ctgcagaact ggtgagtaac agaaacgcca gaatactttt
    18361 tttcatcatt ttcgtaatcc atcaaaaagg ttcgagagtc gcaagcaggc tgatgatcac
    18421 cagcactacc gcacctatcg cccattcgag tttagtcatc cacacaaacc agggtgccgc
    18481 acgatcttca tcctgcctca tgcgcggtac aagaacataa cgattagcca gcgcaatgac
    18541 caccataatc atcaccagga tggctttgag caataagagt tggccccagt aagtcgtaag
    18601 cgtgggggga aatccggtaa tcaacaatgc attaagcacg ccgcttgcca gtacgccgat
    18661 caccgcaaaa tgcccgcacc aggaaaaacg catcagcgcc tgaatggcct ggtgtcgcca
    18721 acggcctttg atgagttgca tacaccagag caccggcagt aatcccccaa accacgccgc
    18781 cgcacaaatc aggtgaatcg cgtgattagt ctgatggatt ttcgccgtta ccccttcatt
    18841 cagcgtcgca tgccccactc ccgccagcag aataaattgc gcggtagtga gcataaacag
    18901 caaacgtggc atattccgcg gttgcataag ggcgacgatc aacgttacga gggcgagaac
    18961 aatttgccat aaccagacgc caccaaactg cgtttgcaaa acagcctgcc aaatgtttgg
    19021 cgaaaataca tctgtccatc ccgttcccat caacccgccc tgaacggcga gcattgcagt
    19081 ggcactgatc aaactccaga cggcagcgtg ctgttgtaaa cgcagaaaac gcttcgccag
    19141 caagcgacga atcgtcaagg gggccagcca ggcaccgtac attgcgaagc caaaaaccag
    19201 catcagcgag gtgaaatgga taaatcgcag cgcgatccag gtaaacgcca gcatgattta
    19261 tttcacgcta aaggtgtagt gtcctttcgt tttatgcccg tccaccgaaa caacatgcca
    19321 gtcaacggtg taggttccgg gtttcagcga atcggccagt ggaacaatca gttgcttttg
    19381 atcctgctca tttcgcttcg ccggtaatgt tttaatattt tcgttttttg gccccgtgat
    19441 ttttgcacca ctgaatcctg tttcaacacc ttccgagaag tttaaagtga ttgcctgcgg
    19501 tgcagctgtc acttgcgcgt ttgccgcagg atactgatgc gttaaatgcg catgtgccca
    19561 gacggaaggt gtcaccaacg aagtggtcag gattgccagc gcgtagcgaa gggagcgtgc
    19621 agttgaagcc atattatcta ttcctttttg taataacttt tttacagagc ataaccttgt
    19681 ctaatgtctg agtcgaggat catcaattcc ggcttgccat cctggctcac tcttagtaac
    19741 ttttgcccgc gaatgatgag gagattaaga atgctgaaga atctggctaa actggatcaa
    19801 acagaaatgg ataaagtgaa tgtcgatttg gcggcggccg gggtggcatt taaagaacgc
    19861 tacaatatgc cggtgatcgc tgaagcggtt gaacgtgaac agcctgaaca tttgcgcagc
    19921 tggtttcgcg agcggcttat tgcccaccgt ttggcttcgg tcaatctgtc acgtttacct
    19981 tacgagccca aacttaaata aaacttatac agagttacac tttcttacat aacgcctgct
    20041 aaattatgag tattttctaa accgcaccca taatttgcag tcattttgaa aaggaagtca
    20101 ttatgtcgtt ctggaaagtt gcagcagcgc aatatgagcc tcgtaaaacc tcactcaccg
    20161 aacaggtagc tcatcatctg gagtttgtga gggccgccgc caggcaacaa tgtcagctgt
    20221 tagtttttcc atcgctttct ttactggggt gtgattattc gcgacgtgcc cttcctgccc
    20281 cacccgatct ctcactgtta gacccgcttt gttatgccgc aacgacctgg cgaatgacca
    20341 tcattgccgg ccttcccgtt gaatataacg atcgctttat tcgtggaatt gcggtgttcg
    20401 caccgtggcg aaaaacgcct gggatctacc atcagagtca tggtgcatgt ctgggccgtc
    20461 gttccagaac catcacggtg gtcgatgaac aaccggaagg tatggatatg gacccgacct
    20521 gttcactgtt tacgacaggg caatgtctgg gagaacccga cctgctggcg tcggcccgtc
    20581 gcttacaatt tttttcacat cagtactcga ttgctgtgct gatggcaaat gcccgtggta
    20641 acagtgcact atgggatgaa tatggtcgtc tcatcgttcg cgccgatcgt ggttcattat
    20701 tgttagtcgg tcagcgttca tcacagggtt ggcaaggcga tatcattcca ttacgctagg
    20761 ctttttcggc ctggagcatg ccatgttgcg cattatcgat acagaaacct gcggtttgca
    20821 gggagggatc gttgagattg cctctgttga tgtcattgac ggaaaaatcg tcaaccccat
    20881 gagccacctg gtgcgccccg atcgtcctat tagtccacaa gcgatggcga ttcatcgcat
    20941 caccgaagcc atggtcgccg ataaaccgtg gattgaagat gtgatcccac actattacgg
    21001 cagtgaatgg tatgtcgcgc acaacgccag ctttgaccgc cgcgtactgc ctgagatgcc
    21061 cggtgagtgg atttgcacca tgaaactggc ccgtcgcttg tggccaggaa tcaagtacag
    21121 caatatggcg ttatataaaa cacggaagct caatgtacag acgccgccgg gcctgcatca
    21181 tcaccgcgcg ttgtatgact gttatatcac cgccgcgttg cttatcgata ttatgaacac
    21241 ctccggctgg acggcagaac agatggccga tatcaccgga cgtccgtcgt tgatgacgac
    21301 cttcaccttt ggcaaatacc gtggcaaagc ggtttccgac gttgccgaac gcgatccggg
    21361 ctatctgcgc tggttattta ataacctgga cagcatgagc ccggagctgc gtttaacact
    21421 gaaacattat ctggaaaata cttaatccgc aggcgtagcg ggtaatgttc cctgcgccag
    21481 cgcgaccaga aaagcatatt ccatcgctac gccttcgtac gatttaaagc ggccagattt
    21541 accgccatgg cctgagtcca tgtcggtaca gagcaataaa agatggttat cggttttcag
    21601 ctcgcgcaat ttagcgaccc attttgccgg ttcccaatat tgcacctgag aatcgtgtaa
    21661 accggtcgtt accagtaaat gcggataagc ctgtgcggtg acgttgtcat acgggctgta
    21721 gcttttcatg tactcgtaat attgcggatc ctgcgggtta ccccactctt caaactcacc
    21781 agtggtaaga ggaattgatt catcaagcat cgttgttaca acatcaacaa acggtacctg
    21841 ggcgataacg ccgtggaata actccgggcg ttgattaatt gcaacgccca tcaacatgcc
    21901 ccccgcactc ccgcccatcg cataacaaag cgaaggagag ccatagccca gttttaacaa
    21961 tgcatcgcag gcatcaagat aatcattaaa cgtatttttc ttcttcagaa attttccgtc
    22021 ttcgtaccat tgttgcccca gctcaccacc gccgcgaaca tggacaatgg cgtagacaaa
    22081 gccacgatct aacaaactca agcggctaaa actgaaatcg gcatcaatac ttgcgccgta
    22141 agaaccatag ccatacacca gcaacgggtt gtgtcctttg cgaaaatgtt tgcgatggta
    22201 gaccaacgaa accggaactt cgacgccatc acgggcgact atccacaggt gttcactgcg
    22261 gtaattcgcc gcatcaaaac caggaacttc cgtttgtttt aatacacgac gctcaccggt
    22321 atccatatcc agttcaaaca aagtgtctgg tgtggtcatg gaagaataac cataacgcaa
    22381 tcgcgcggtt tcaggttctg gattgtaggc aatccaggtc acataggctg gatcatcaaa
    22441 ggcaatacca atgacttccc gggtcttgcg gttaatttgg cgcaaactgg ttaacccgcg
    22501 ctgacgctct tcaaccacca gccagtcggt aaacagcgta aacccttcca gcatgatgtt
    22561 ttcgcgtggc ggaattaact cttcccactg ttgctcatca cgcatacggg tacggtataa
    22621 gccaaagttt ttgccgtggc ggttggaacg cagataaaaa cgatgctggt agtgatcaag
    22681 gctgtattcg tgatctttgc ggcgcggcag aaaaacaaac ggctcggcat cggccatttc
    22741 cgcgtccagc aggcgaactt cactggtggt ggcgctggcc aaatgaataa ctacatagtg
    22801 cttcgaggtc gttttatgca ggctgacgta ataggtatcg tctttttctt cgtaaatcag
    22861 tttatcttgc gatgctggcg taccgatggc gtgacgccag acctgataag gcagcagcgt
    22921 caccggatgc ttgcgaacat agtagaaagc aggagtcatt tgcccagaca aagctgggtt
    22981 caacgttatc cagcagttcc gggtaccagt tacctgtttc cagattacga aaacgaatgc
    23041 cgtactggcg tcgggaaaga aaatcttctg ccagcgccat gatggtgtta tcgggcgtaa
    23101 tcgccattcc gcccatcgaa taaaactcac tatgagctgc gcgcttattg gcatcgagca
    23161 atgtttccca ctcatcccac tcttcactga atgccgattg acgctggtag atagcatatt
    23221 cacagcctgg ttcataaata tgccgatagc ggtagccatt tttgatgtag ggcgcagaaa
    23281 cttctcgttg cggaatgcgg tcgatgattt cctttaagat gcgatcctgc aaggcttgtt
    23341 gtgaggccat cacccgatga ccgtaactat tttcttgttg caggtagtcc aggacttctg
    23401 gctgagaacg cgtatcgtcc cgcagccagt agtaattatc gatgcgcgta tcgccatgaa
    23461 gcgtcatggc gtggggaatg cgggcggctt ttggtagcat gttattgttc tttctggttg
    23521 aaacatctta taagggtggc aaaactcacc ggggatgcaa gcgaaacagg gtcgtcattg
    23581 cttagatgat gacaggtaat ggcgcggata tcgaatgtta tgcagacaga gaaaatcagc
    23641 ctgttcaaat ggctgtgcgg ttctggatag cccgaaatag tcaacttcag gctatccaga
    23701 gagcggaatt attccgccaa agtgcgtttt tgctgttcga gatcgcgttc aatgccttca
    23761 cgaacatcct gggggatttt cagcgcgtca cccagtgcat tcaggtaact gcgttccata
    23821 aaatggtcaa tatcaatagc cgcgcaactc aggaaataga tttccagcgc ctcttcttca
    23881 ttgcggaccc cggtagccag gcgttgtgga tccagcggtt gttcgattgc ctgctcaatg
    23941 agtacacgcc cctgcccttc cacgccagct tcacgcaatt gctggtcgat tgccgcacgt
    24001 tctttggcat caatatgacc atcactttta gcggcaaaga ccagcgcaag gatcaaacgt
    24061 tctgtacgct catccagcgg cgtactttgc gcgccaaact gcggttcgtc ctgatgcgcc
    24121 gcgcgaattt tatctttgta tttattccac agcaccgtac ccgctaccgc tccgccgcca
    24181 accagtaacg cgttggtgcc atatttcgta agaagtttac gtgctgattt atttgcgacc
    24241 agcagcccag ccagtccgcc taatgcgcct ggcactaaca gtttgcccaa tccctgatcc
    24301 gtagacgagg aggtagaaga actgctttgc ccaagcaggg attgcagttg atttaaccag
    24361 ttagccatat ttgctcctca gtaaccattt ttagtgcctg tagcatagcg gatgaggatg
    24421 tcagaaaatg tatatcgaga caaaagatgc gcaaataagc ggggcggtag gccgttattc
    24481 aaagaaattc gcgccaggta aggtatttac tgtatatcag gtgataaggg tattttaggt
    24541 gaaagtagtt tgtcgttttg agtggcattg tttgatgctg attgcccgat gcgacgctgt
    24601 tgcgtcttct caggcctacg actgtggtgc gttgtggggc ggacaaggca ctcgcgccgc
    24661 atccgccaac ggtcaatcga attatcagaa gccattaacg ccgctgatat tccgccattc
    24721 ccgcttcgca gtccacgctg acctggtaat gaatatcggc acttttacca cgcacggtta
    24781 gcggtactga ccatttatca tctttaccct gaatgtcctg caaactgacc caagccacgg
    24841 gatcggcctg accgacaatt ttttgatcat ctgcccaacg cgccacgcga ttttgttgat
    24901 aatcacgttt tacgctcgcg gcaattccgg cagcatccag accttcacac tttgggaaag
    24961 tgaccgactt gctggtttca ttattggcag cgaaaactga tgcgcaggca gaaaccaaca
    25021 acagccctaa aaacgcccct ctttttttca tgtttttctc catagcacaa tgattcagga
    25081 gaaagcatgg tacaaattgt caggagcgca agttgcttca ggccgcgtgt gaggcatctt
    25141 cctcttcgga ttcagcaccg acgacctgtg gggacggtaa tttgccggtt ttcagaatgg
    25201 tgctcagcac atctttttgt tctgccagcc atagcgaaag ggcttcacgt tgctcgtctt
    25261 ccatttgcac tggagaattt gtcagccagg tgtcaagcag atccgccgta tcaagcattt
    25321 tgtcataagc atcggcttcc tttttgctgg taaacgacat tttctcttcg ccctcacgaa
    25381 tgactacgta tttaacttca accgccattt gcagcctctc ataataactg tgattttata
    25441 cagtatattt cttttcggtt gagaaatcaa catcagcaat aaagacacac gcaaacgttt
    25501 tcgtttatac tgcgcgcgga attaatcagg ggatattcgt tatgacgtta ttaggcactg
    25561 cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc cggtgaactg ggtaaagaag
    25621 tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc cgtcgatcgc tatgccgacg
    25681 caccagccat gcatgtcgcg catcgctccc atgtcattaa tatgcttgat ggtgatgcat
    25741 tacgccgtgt ggttgaactg gaaaaaccac attatatcgt gccggagatc gaagctattg
    25801 ccaccgatat gctgatccaa cttgaagagg aaggactgaa tgttgtcccc tgcgctcgcg
    25861 caacgaaatt aacgatgaat cgcgagggta tccgtcgcct ggcggcagaa gagctgcagc
    25921 tgcccacttc cacttatcgt tttgccgata gcgaaagcct tttccgcgag gcggttgccg
    25981 ccattggcta tccctgcatt gtaaaaccgg tgatgagctc ttccggcaag gggcagacgt
    26041 ttattcgctc tgcagagcaa cttgctcagg catgggaata cgctcagcaa ggcggtcgcg
    26101 ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt tgacttcgaa attaccctgc
    26161 taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc agtaggtcat cgccaggaag
    26221 atggcgacta ccgtgaatcc tggcaaccac agcaaatgag cccgcttgcc cttgaacgtg
    26281 cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg ttatggtctg tttggtgtcg
    26341 agctgtttgt ctgtggtgat gaggtgattt tcagtgaggt ctcccctcgt ccacatgata
    26401 ccgggatggt gacgttaatt tctcaagatc tctcagagtt tgccctgcat gtacgtgcct
    26461 tcctcggact tcctgttggc gggatccgtc agtatggtcc tgcagcttct gccgttattc
    26521 tgccacaact gaccagtcag aatgtcacgt ttgataatgt gcagaatgcc gtaggcgcag
    26581 atttgcagat tcgtttattt ggtaagccgg aaattgatgg cagccgtcgt ctgggtgttg
    26641 cgctggcgac cgctgagagt gttgttgacg ccattgaacg cgcgaagcac gccgccggac
    26701 aggtaaaagt acagggttaa acccggcaaa aaaacgctac aaaaatgccc gatcgtagat
    26761 cgggcgtttt gacttttaca gcttagcgcc ttctacagct tcacgcgcca gcttagtaat
    26821 gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc agccaggaac caccgatgca
    26881 cagcacgctt ttcagcgcca ggtagtcacg gtagttagcc ggagaaatac cacccgtcgg
    26941 gcagaaacgg acctgggaga acggacccgc gatcgcctgc agggctttca cgccgccgtt
    27001 agcttcagcc gggaagaatt tgaactcttt caaaccgtag tccataccca gcatcagttc
    27061 ggaaacagtg ctgatccccg gaattagagg aatagtccct tcggtagcag ctttcagcag
    27121 cggctcggtc agacccgggc taattgcgaa ctgtgcaccc gcttcagtga cttctgccag
    27181 ctgctgtgga ttcagcaccg taccggcacc cacaatcgct tcaggcactt ctttggcgat
    27241 agcacggata gcgtcaactg cacactcggt acgcagagtc acttccagaa cgcgcacccc
    27301 accagcaacc aacgcttttg ccatcggcac tgcgtgttcc agttttttta ccacgataac
    27361 cggtacaacc gggccggtgg tcaggattga ttctgcactt gttttccagt ttttcatcag
    27421 agttttctct cgcctgatta caaatttgtc gtcttaaaaa gtgatacagg ttgcgccctg
    27481 ttcggcaccg gacagttttt cacgcaaggc gctgaataat tcacgtcctg tccccacgcg
    27541 tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc agttccgctt cgtctaccag
    27601 cagcgtcagt tcgcctgtct gtccattcac acgaatgatg tctccgtcgc gcacttttgc
    27661 cagcagcccg ccatcgtagg cttctggtgt tacgtggata gctgacggca ctttacctga
    27721 agcgccggag agtcgtccat cggtaactaa cgcaattttg aaacaccggt ccaataatac
    27781 accaagtggc ggcatgagtt tatgtaattc tggcattccg ttcgcttttg gcccctgatg
    27841 acggacaaca acgacacaat cgcggtccag caaacccgct tcaaaggccg gcataacgtc
    27901 atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc tggttctcaa ccggcacggc
    27961 agaggttttc ataaccgcac ggcccaggtt accgcttaac acttttgtcc caccatgatg
    28021 agagaaaggt tgttcgaagg aagcgatcac attgctgtcg agtgattttt ccgccccttc
    28081 ccgccagtcc agttcaccat tattcagcca tggttcaagg gtataacgag acagaccaaa
    28141 gcctgccacc gtattgacat cttcatgcag caggcctgct ttgagcagtt cacgcaccag
    28201 aaccggtacg ccacctgccg cctggaagtg gttaatatcg gccggaccgt tcgggtagag
    28261 acgtgccatc agcggtacaa catcagaaag gtcagagaag tcatcccagt taatctgaat
    28321 accggccgcg cgcgccatcg ccaccaggtg catggtgtgg ttagtggaac caccggtcgc
    28381 cagcagtgca acgataccgt tcaccaccac tttctcatcg atcatcttac cgatcggcat
    28441 ccattcatta ccattaccgg tcatgcgtgt aacctgacgc gcagcggcag cggtcaaagc
    28501 atcgcgcagc ggagaatccg gatgaacaaa agaagagcct ggcaactgca tccccataaa
    28561 ttccaccacc atctggttgg tgttggcagt accgtagaaa gtacatgttc ccggcgcatg
    28621 gtaagacgcg gcttctgact ccagtaaggc catgcggtcc actttacctt cggcataaag
    28681 ctggcgaata cgcacttttt ctttatttgg caaaccgctt gccatcggtc cagacggcac
    28741 aaacaccgca ggcaaatgac caaacgacag ggctgccatc gtcagacccg ggacaatctt
    28801 gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta tgggacagcc ccaccgccgc
    28861 agacatcgct atcacttcgc ggcttagcag cgacaattcc attccatcct gcccctgggt
    28921 gacaccatca cacatcgccg gaacaccgcc cgcaacctga ccaaccgcat tcgcttcatg
    28981 cagggcttta cgaatgattt ctggatagtg ttcataaggc tggtgcgcgg agagcatgtc
    29041 gttataggag gtgatgatgg cgatattgtt acgcaacatg cttttcaaag aggctttgtc
    29101 ttctggctgg caggcagcga aaccgtgtgc caggttaccg catgccaact gcgaacgatg
    29161 aacggtcgac gttttcgctt gttctatccg ggcgagataa gcagagcgag tctcgcgcga
    29221 acgttcaata attcgatttg ttacgcgtaa caattgtgga ttcataaagg ctcctgaaat
    29281 tgagttgtca gagcaggatg attcacaacg cgtttcattc agaggattta tgagtgaaac
    29341 gcctgtaacc ggagctcata gggcaaaaac gtttcagtca gtgtaataaa aaaagcctcg
    29401 tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt ctacaatctg tgcaagatca
    29461 tgttaccggt aaaataacca taaaggataa gcgcagatat tactcaaact cattccagga
    29521 acgaccatca cgggtaatca tcgccaccga ggcaacgggt ccccaggttc cggcctgata
    29581 cggtttcggc gcatcattgt ccatcgccca cgcctcagta atggagtcta cccatttcca
    29641 ggcttcttcc acttcatcac gacgtacaaa cagtgcctga ataccacgca tggtttccag
    29701 cagcagacgt tcataggcat ccgccagatg cgtctgatta aaggtttctg aatagctcag
    29761 atccagcttg gtgatttgca ggttatgttt gtggtcaagg ccaggaactt tattcagtac
    29821 ctggatatcc acgccttcat caggttgcag acggatagtc agtttattct gcggcagatc
    29881 ctgccacgat tctttaaaca gattcagttc aggtgttttg aaatagacca cgacttcaga
    29941 acatttggtc ggcagacgtt taccagtacg caggtagaat ggcacaccgg cccagcgcca
    30001 gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta ttgctgctct tgttcgcgcc
    30061 ctcttcttcc agatatcccg gcactttttt gccctgggcg aagcccgcag tatattgccc
    30121 gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg cgcagagact tcagtacttt
    30181 cactttttca tcgcggatgc tgtctgcgct caggtcagac ggcggagaca tcgcaatcat
    30241 gcagagaatt tgcagcaggt ggttttggat catatcgcgc atctgaccgg ctttatcaaa
    30301 atagccccag cgcccttcga tccccacttc ttctgccacg gtaatctcaa catgatcaat
    30361 ggtgcgattg tcccagttat tcacaaacag ggagttagca aaacgcagcg ccaacaggtt
    30421 cagcaccgtt tctttaccaa gatagtggtc gatacggtaa acctggcact cctcgaagta
    30481 ttcgccaacc tgatcattga tttcctgcga ggtcgccagc gacgtcccca gcggtttctc
    30541 catgactacg cgtgccggtt tagcattcag tttagcctcg ccaagccctt tgcaaattgc
    30601 gccaaaagtg ctgggcggca tggcaaagta gttaatggtg atacgatttt tttgatccag
    30661 catcgcgccg agacggctga atgcagcagt gtcattgaca tcgagattac aaaaatccag
    30721 acgtgcactc agggtgtccc ataaaccttc atcaatggtt tctttcatga aagtttcgag
    30781 cgcctcgcgg acaactttgg tatatgccgc tttatcccag tcagcacgcc ctacgccgat
    30841 aatccgggtg tccgggttga gctgaccggc tttttccagt tgatacaggg aaggcagcaa
    30901 tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc aggtcacagg cctgggctgt
    30961 ttgcgttacc gccatgtcat tctccttaag ttaactaacc cggtacttaa gccagggtat
    31021 acttgtaatt ttcttacggt gcactgtact gcttttacga gcttgcgaaa actgtaaacg
    31081 cttatccacc cgtgcgatta cggtaaaagc gcgcaaagtg cggcaaaact gataaaaaat
    31141 catcgttttc cattgggtaa aaatctgaca ctgatcatgt tatgaaaaaa aataacaact
    31201 tttttatctg cttttgtcat taacacagca cacaggcgta atatctgaca aaactacatt
    31261 tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt ttctttacat catgaatatg
    31321 ctggaaaaaa tccagtctca gctggaacat ttgagcaaat cagagcgcaa agttgccgag
    31381 gtcattctgg cttcgcccga taacgcgatc cattcgagta ttgctgctct ggcgctggaa
    31441 gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac gcgcggtttt
    31501 cctgatttta aacttcatct ggcacaaagt ctggcgaatg gcactcccta tgttaatcgc
    31561 aatgtcaatg aagatgacag cgttgaatca tacacaggga aaatatttga gtccgcaatg
    31621 gcaacgcttg atcatgtccg tcattcactg gataaatctg ccatcaaccg cgccgtcgac
    31681 ttgctcactc aggcaaaaaa aatcgccttt ttcggattag gctcttcagc cgccgttgcc
    31741 cacgatgcga tgaataagtt ctttcgtttt aatgttccgg tggtgtactc cgatgatatc
    31801 gtgctgcaac gcatgagttg tatgaattgt agcgacggag acgtggtggt gctgatttct
    31861 cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc tggctcgcga aaacgacgca
    31921 atggtgattg ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac gctggcaatt
    31981 accctcgacg taccggaaga tactgacatt tatatgccca tggtttctcg acttgcacag
    32041 ctgaccgtga tagatgtgct ggcgacagga tttactttgc gacgcggtgc aaaattcaga
    32101 gataacttga agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa gcagttactt
    32161 aatttaagtg acgatcgcta aaaacgactg tcactgtcct aatcttatac gacatccgaa
    32221 tgagattaat ttatcgccat cgcggcgtta tttcattcgg atttcatgtt caagcaacac
    32281 ctggttgttt cagtcaacgg agtattacat gtccagaagg cttcgcagaa caaaaatcgt
    32341 taccacgtta ggcccagcaa cagatcgcga taataatctt gaaaaagtta tcgcggcggg
    32401 tgccaacgtt gtacgtatga acttttctca cggctcgcct gaagatcaca aaatgcgcgc
    32461 ggataaagtt cgtgagattg ccgcaagact ggggcgtcat gtggctattc tgggtgacct
    32521 ccaggggccc aaaatccgtg tatccacctt taaagaaggc aaagttttcc tcaatattgg
    32581 ggataaattc ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag aaaaagtcgg
    32641 tatcgactac aaaggcctgc ctgctgacgt tgtgcctggc gacatcctgc tgctggacga
    32701 tggtcgcgtt cagttaaaag tactggaagt tcagggcatg aaagtgttca ccgaagtgac
    32761 cgtcggtggt cccctctcca acaataaagg tatcaacaaa cttggcggcg gtttgtcggc
    32821 tgaagcgctg accgaaaaag acaaagcaga cattaagact gcggcgttga ttggcgtaga
    32881 ttacctggct gtctccttcc cacgctgcgg cgaagatctg aactatgccc gtcgcctggc
    32941 acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg aagccgtttg
    33001 cagccaggat gcaatggatg acattattct cgcctctgac gtggtaatgg ttgcacgcgg
    33061 cgacctcggt gtggaaattg gcgacccgga actggtcggc attcagaaag cgttgatccg
    33121 tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc cagatgatgg agtcaatgat
    33181 tactaacccg atgccgacgc gtgcagaagt catggacgta gcaaacgccg ttctggatgg
    33241 tactgacgct gtgatgctgt ctgcagaaac tgccgctggg cagtatccgt cagaaactgt
    33301 tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca acgtttctaa
    33361 acaccgtctg gacgttcagt tcgacaatgt ggaagaagct attgccatgt cagcaatgta
    33421 cgcagctaac cacctgaaag gcgttacggc gatcattacc atgaccgaat cgggtcgtac
    33481 cgcgctgatg acctcccgta tcagctctgg tctaccaatt ttcgccatgt cgcgccatga
    33541 acgtacgctg aacctgactg ctctctatcg tggcgttacg ccggtgcact ttgatagcgc
    33601 taatgacggc gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata aaggttactt
    33661 gatgtctggt gacctggtga ttgtcaccca gggcgacgtg atgagtaccg tgggttctac
    33721 taataccacg cgtattttaa cggtagagta atctcacctg ccccggccgc tgatgaccgg
    33781 ggtatttagc aacacataaa ttaggactac tcattcaatc caatcataat cacagccccc
    33841 tgtaaaatga atacagggat agcctgaatg gagatctaaa tagcttgcaa gacgtcgcat
    33901 acccgcttcc tgtgcaacac tatatccaat aactgccatc ggtaagccac tatctttgga
    33961 gtattgggtc tgcacccatg ttttaattga atcgttaatc gtaatataat aatcagcctg
    34021 atattccata tattgtattg gatcacaaaa acagcctgcc ccaatcccaa tagttctaac
    34081 tattctggct ctatctccat aaaattggat cccaggaaga ttataaattt tcaaattttt
    34141 ggcaaaacgt ttggtaatat taatggcact gtcaggttct atagcatata catggtacat
    34201 atcatcagaa gcaacaatat ttgtaacatc cagttctaac tgctgagcca aagccttaga
    34261 cattccgaac gtaggctctc gatccatgac atcatggcaa cgaatgatta cca
//
LOCUS       DAZKIV010000027        34199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZKIV010000027 DAZKIV010000000
VERSION     DAZKIV010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.027"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(237..449)
                     /gene="rpmE"
                     /locus_tag="ACHOT6_001424"
     CDS             complement(237..449)
                     /gene="rpmE"
                     /locus_tag="ACHOT6_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFP9946666.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            652..2850
                     /gene="priA"
                     /locus_tag="ACHOT6_001425"
     CDS             652..2850
                     /gene="priA"
                     /locus_tag="ACHOT6_001425"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFP9946667.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVAELEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3006..4031
                     /gene="cytR"
                     /locus_tag="ACHOT6_001426"
     CDS             3006..4031
                     /gene="cytR"
                     /locus_tag="ACHOT6_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFP9946668.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4123..5082
                     /gene="ftsN"
                     /locus_tag="ACHOT6_001427"
     CDS             4123..5082
                     /gene="ftsN"
                     /locus_tag="ACHOT6_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFP9946669.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5175..5705
                     /gene="hslV"
                     /locus_tag="ACHOT6_001428"
     CDS             5175..5705
                     /gene="hslV"
                     /locus_tag="ACHOT6_001428"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFP9946670.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5715..7046
                     /gene="hslU"
                     /locus_tag="ACHOT6_001429"
     CDS             5715..7046
                     /gene="hslU"
                     /locus_tag="ACHOT6_001429"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFP9946671.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7113..8039
                     /gene="menA"
                     /locus_tag="ACHOT6_001430"
     CDS             7113..8039
                     /gene="menA"
                     /locus_tag="ACHOT6_001430"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFP9946672.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8132..8617
                     /gene="rraA"
                     /locus_tag="ACHOT6_001431"
     CDS             8132..8617
                     /gene="rraA"
                     /locus_tag="ACHOT6_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFP9946673.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8702..8947)
                     /gene="zapB"
                     /locus_tag="ACHOT6_001432"
     CDS             complement(8702..8947)
                     /gene="zapB"
                     /locus_tag="ACHOT6_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFP9946674.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9372..10217
                     /gene="glpF"
                     /locus_tag="ACHOT6_001433"
     CDS             9372..10217
                     /gene="glpF"
                     /locus_tag="ACHOT6_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFP9946675.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10240..11748
                     /gene="glpK"
                     /locus_tag="ACHOT6_001434"
     CDS             10240..11748
                     /gene="glpK"
                     /locus_tag="ACHOT6_001434"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFP9946676.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11883..12893
                     /gene="glpX"
                     /locus_tag="ACHOT6_001435"
     CDS             11883..12893
                     /gene="glpX"
                     /locus_tag="ACHOT6_001435"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9946677.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            12990..13736
                     /gene="fpr"
                     /locus_tag="ACHOT6_001436"
     CDS             12990..13736
                     /gene="fpr"
                     /locus_tag="ACHOT6_001436"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFP9946678.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13741..14169)
                     /gene="uspD"
                     /locus_tag="ACHOT6_001437"
     CDS             complement(13741..14169)
                     /gene="uspD"
                     /locus_tag="ACHOT6_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFP9946679.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14196..14495)
                     /gene="yiiS"
                     /locus_tag="ACHOT6_001438"
     CDS             complement(14196..14495)
                     /gene="yiiS"
                     /locus_tag="ACHOT6_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFP9946680.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14707..15147)
                     /gene="yiiR"
                     /locus_tag="ACHOT6_001439"
     CDS             complement(14707..15147)
                     /gene="yiiR"
                     /locus_tag="ACHOT6_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9946681.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15248..15847
                     /gene="yiiQ"
                     /locus_tag="ACHOT6_001440"
     CDS             15248..15847
                     /gene="yiiQ"
                     /locus_tag="ACHOT6_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFP9946682.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            15955..16722
                     /gene="tpiA"
                     /locus_tag="ACHOT6_001441"
     CDS             15955..16722
                     /gene="tpiA"
                     /locus_tag="ACHOT6_001441"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFP9946683.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16777..17532)
                     /gene="cdh"
                     /locus_tag="ACHOT6_001442"
     CDS             complement(16777..17532)
                     /gene="cdh"
                     /locus_tag="ACHOT6_001442"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFP9946684.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17639..18628)
                     /gene="sbp"
                     /locus_tag="ACHOT6_001443"
     CDS             complement(17639..18628)
                     /gene="sbp"
                     /locus_tag="ACHOT6_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFP9946685.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQICKR"
     gene            complement(18948..19910)
                     /gene="pfkA"
                     /locus_tag="ACHOT6_001444"
     CDS             complement(18948..19910)
                     /gene="pfkA"
                     /locus_tag="ACHOT6_001444"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFP9946686.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20091..20993)
                     /gene="fieF"
                     /locus_tag="ACHOT6_001445"
     CDS             complement(20091..20993)
                     /gene="fieF"
                     /locus_tag="ACHOT6_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFP9946687.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21143..21643)
                     /gene="cpxP"
                     /locus_tag="ACHOT6_001446"
     CDS             complement(21143..21643)
                     /gene="cpxP"
                     /locus_tag="ACHOT6_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFP9946688.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21793..22491
                     /gene="cpxR"
                     /locus_tag="ACHOT6_001447"
     CDS             21793..22491
                     /gene="cpxR"
                     /locus_tag="ACHOT6_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFP9946689.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22488..23861
                     /gene="cpxA"
                     /locus_tag="ACHOT6_001448"
     CDS             22488..23861
                     /gene="cpxA"
                     /locus_tag="ACHOT6_001448"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFP9946690.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(23967..24641)
                     /gene="yiiM"
                     /locus_tag="ACHOT6_001449"
     CDS             complement(23967..24641)
                     /gene="yiiM"
                     /locus_tag="ACHOT6_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFP9946691.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENISTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24790..25773)
                     /gene="kdgT"
                     /locus_tag="ACHOT6_001450"
     CDS             complement(24790..25773)
                     /gene="kdgT"
                     /locus_tag="ACHOT6_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFP9946692.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26033..26653)
                     /gene="sodA"
                     /locus_tag="ACHOT6_001451"
     CDS             complement(26033..26653)
                     /gene="sodA"
                     /locus_tag="ACHOT6_001451"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFP9946693.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            26938..27972
                     /gene="rhaT"
                     /locus_tag="ACHOT6_001452"
     CDS             26938..27972
                     /gene="rhaT"
                     /locus_tag="ACHOT6_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFP9946694.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLITHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(27969..28817)
                     /gene="rhaR"
                     /locus_tag="ACHOT6_001453"
     CDS             complement(27969..28817)
                     /gene="rhaR"
                     /locus_tag="ACHOT6_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFP9946695.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFNASAGQPHWRLGSMGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28891..29727)
                     /gene="rhaS"
                     /locus_tag="ACHOT6_001454"
     CDS             complement(28891..29727)
                     /gene="rhaS"
                     /locus_tag="ACHOT6_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFP9946696.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30015..31484
                     /gene="rhaB"
                     /locus_tag="ACHOT6_001455"
     CDS             30015..31484
                     /gene="rhaB"
                     /locus_tag="ACHOT6_001455"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFP9946697.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWDVDSLESAIRLGLNMVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLINQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31481..32740
                     /gene="rhaA"
                     /locus_tag="ACHOT6_001456"
     CDS             31481..32740
                     /gene="rhaA"
                     /locus_tag="ACHOT6_001456"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFP9946698.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYLGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            32982..33806
                     /gene="rhaD"
                     /locus_tag="ACHOT6_001457"
     CDS             32982..33806
                     /gene="rhaD"
                     /locus_tag="ACHOT6_001457"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFP9946699.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33816..34130
                     /gene="rhaM"
                     /locus_tag="ACHOT6_001458"
     CDS             33816..34130
                     /gene="rhaM"
                     /locus_tag="ACHOT6_001458"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFP9946700.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8364 a         8893 c         8884 g         8058 t
ORIGIN      
        1 accccggctg aaccctggac aatgctaccg ccatactgcg tcatgtcgcc ctgacgcgcc
       61 gccggttttc cgctcatcgt aaaatccttt tgaaataaat aagaaccaga agcctagcgc
      121 cagtcaacaa caggaaatga taatgagtta acaccccgga atttttcaga atttataaaa
      181 ggcaaattac aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
      241 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
      301 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
      361 tgaccaacgg tggagcggat tttcattacg ttaccgcaag agcagctagc agtaatttct
      421 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
      481 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
      541 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
      601 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
      661 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
      721 atgacggtta aagccgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
      781 ggggttgtgg tgtcagttag cgatgtcagt gagctgccgc ttaacgagct gaaagcggta
      841 gttgaagtgc tggacgttga gccggtattt actcactccg tctggcggtt actgctctgg
      901 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
      961 ctgcgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
     1021 ggtcaggcgg tggatcttaa cagcctgaaa cgttcgccaa agcaacaaca ggcgctggcg
     1081 gcgttacggc aaggcaaaat ctggcgcgac caggtcgcgg aacttgaatt taatgatgcc
     1141 gcgttgcagg cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
     1201 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacaa
     1261 gccaccgccg ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg
     1321 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
     1381 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
     1441 cgttttcgtg aacgttttaa tgcccccgtg gaagttctgc attccggcct gaacgacagc
     1501 gagcggcttt cggcgtggtt gaaagcgaaa aatggcgagg cggcgattgt gatcggcacc
     1561 cgctccgcgc tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga tgaagagcac
     1621 gacagctcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat
     1681 cgtgcgcata gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg
     1741 ttatgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacccgtcg ggcagggaat
     1801 gcgcgtccgg caattcaaca tgtgctggat ttaaaaggtc agaaggtgca ggcaggtctg
     1861 gctccggcgt taatcacccg tatgcgccag catttacagg ccaacaatca ggttatcctc
     1921 tttcttaatc gtcggggttt tgcgcctgcg ctgctgtgcc acgactgcgg ctggatagcc
     1981 gaatgcccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
     2041 caccactgtg acagtcagcg tccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
     2101 ctggtccccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gatgttcccc
     2161 ggcgtgccca tttcacgtat cgaccgcgat accaccagcc gcaaaggggc gctggaacag
     2221 caactggcgg aagtgcatcg cggcggcgcg aggatcttga ttggcacgca aatgctggcg
     2281 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
     2341 ttttctgccg attttcgctc ggcagaacgt ttcgcccagc tttacaccca ggtcgccggt
     2401 cgtgccgggc gtgcgggtaa acagggcgaa gtggtgctgc aaacgcacca tccggaacat
     2461 cctctgttgc aaacgttgct ctataaaggc tacgacgcct ttgccgaaca ggcgctggct
     2521 gagcgacgca tgatgcagct gccgccgtgg accagccatg tgattgtgcg tgcggaagat
     2581 cataacaatc agcatgcgcc gctgttcctg caacaactgc gtaatctgat cctttccagc
     2641 ccgctggcag acgacaaact gtgggttctc ggcccggttc cggctctggc acctaagcgt
     2701 ggtggccgct ggcgctggca gatcttgttg cagcaccctt cccgcgtgcg cttgcaacac
     2761 atcattagtg gtacgctggc gcttattaat acaataccgg attcccgcaa ggtgaaatgg
     2821 gtgttggatg tcgatcccat cgaaggctaa tgcgcaaacg ttgcgaggcg gatcgaaaaa
     2881 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
     2941 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
     3001 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
     3061 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
     3121 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg
     3181 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
     3241 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
     3301 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
     3361 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
     3421 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattc gcaccggaac
     3481 tggagctacc tacagttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatc
     3541 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccgctgt
     3601 gtcactaccg cctgcaaggt tacgttcagg cgctgcgtcg ctgcggcatt atggttgatc
     3661 cgcaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagcg atgcagcagc
     3721 tgcttgatct tccacaaccg cctactgctg tcttctgcca tagcgatgtg atggcgctcg
     3781 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
     3841 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
     3901 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
     3961 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
     4021 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
     4081 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
     4141 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
     4201 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
     4261 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
     4321 agccagaaag tgaccggtaa cggactacca ccaaaaccag aagaacgctg gcgctacatt
     4381 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
     4441 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
     4501 gctgatatgc gccagcagcc aacgcagctg gttgaagttc cgtggaatga acagacgccg
     4561 gaacagcgtc agcaaacgct acaacgccag cgtcaggcgc agcaattagc ggagcagcaa
     4621 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
     4681 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cggcttctac tcagcagccg
     4741 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
     4801 gcgccggttg ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
     4861 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
     4921 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
     4981 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
     5041 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
     5101 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
     5161 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
     5221 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
     5281 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
     5341 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gtcaaagccg
     5401 ccgttgagct ggcgaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
     5461 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
     5521 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgtg
     5581 cactgttaga aaatactgaa ctgagtgctc gtgaaattgc tgaaaaggcg ttggatattg
     5641 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
     5701 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
     5761 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
     5821 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
     5881 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
     5941 cgccgttcat caaagttgaa gcgaccaaat tcaccgaagt gggctacgtc ggtaaggaag
     6001 tggattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
     6061 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
     6121 tcccacctgc taaaaacaac tggggacaga ccgaacagca gcaggaaccg tccgctgctc
     6181 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
     6241 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccaggcatg gaagagatga
     6301 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
     6361 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
     6421 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
     6481 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
     6541 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
     6601 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
     6661 ctgacctgat cccggaactg caaggtcgtc tgccaatccg cgttgaactg caggcgctga
     6721 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
     6781 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaagcgca
     6841 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
     6901 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
     6961 aaactatcat cattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
     7021 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
     7081 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
     7141 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
     7201 tcgggacagc gctggcatgg tggcaaggtc acttcgatcc gctggtcgcc ctgctggcac
     7261 tgattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
     7321 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
     7381 ttacccagca agagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
     7441 ggctggcact ggttgcagtg gcatgccata cgctggccga ttttgtcggt ttcctgattc
     7501 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
     7561 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttgagt gtcatgggga
     7621 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
     7681 gcctgctggc aacggcagta ctgaatatta ataacctgcg tgatatcaat agcgaccgcg
     7741 aaaatggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
     7801 cctgcctgct aatgggctcg ctggtgtgtc tggcgctgtt taatctattt tcgctgcata
     7861 gcctgtgggg ctggctgttc ctgctggcgg caccattact ggtgaagcaa gcccgttatg
     7921 tgatgcggga aatggacccg gtggcgatgc gaccaatgct ggaacgtact gtcaagggag
     7981 cgttactgac taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac
     8041 tgacaaatat caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg
     8101 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
     8161 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
     8221 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
     8281 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
     8341 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
     8401 ggtcatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
     8461 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
     8521 cgtcaatttt ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg
     8581 gattattctt tctgaagatc cgctggatat tgaatgatag aaaaggcacc gcaaggtgcc
     8641 ttttttctgc gttacctgtt ggcctacaca gtaaagaaat tacgcggaag atgaagcgta
     8701 atcagacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct
     8761 gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa
     8821 cttcctgcga cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag
     8881 tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca
     8941 ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaatcctcgt cccgattacc
     9001 ggtgacgcct taataaatac gagcgcactt tagttagctc cgattgtatg aagccgcgcc
     9061 atcgctgtcc agcggcacgc cttgcagatt acggtttgcc acacttttca tccttctcct
     9121 ggtgacataa tccacatcaa tcgaaaatgt taataaattt gttgcgcgaa tgatctaaca
     9181 aacatgcatc atgtacaatc agatggaata aatggcgcga taacgctcat tttatgacga
     9241 agcacacaca ttttaagttc gatatttctc gtttttgctc gttaacgata agtttacagc
     9301 atgcctacaa gcatcgtgga ggtccgtgac tttcacgcat acaacaaaca ttaactcttc
     9361 aggatccgat tatgagtcaa acatcaacct tgaaaggcca gtgcattgct gaattcctcg
     9421 gtaccgggtt gttgattttc ttcggtgtgg gttgcgttgc agcactaaaa gtcgctggtg
     9481 cgtcttttgg tcagtgggaa atcagtgtca tttggggact gggggtggca atggccatct
     9541 acctgaccgc aggggtttcc ggcgcgcatc ttaatcccgc tgttaccatt gcattgtggc
     9601 tgtttgcctg tttcgacaag cgcaaagtta ttccttttat cgtttcacaa gttgccggcg
     9661 ctttctgtgc tgcggcttta gtttacgggc tttactacaa tttatttttc gacttcgagc
     9721 agactcatca cattgttcgc ggcagcgttg aaagtgttga tctggctggc actttctcta
     9781 cttaccctaa tcctcatatc aattttgtgc aggctttcgc agttgagatg gtgattaccg
     9841 ctattctgat ggggctgatc ctggcgttaa cggacgatgg caacggtgta ccacgcggcc
     9901 ctttggctcc cttgctgatt ggtctactga ttgcggtcat tggcgcatct atgggcccat
     9961 tgacaggttt tgccatgaac ccagcgcgtg acttcggtcc gaaagtcttt gcctggctgg
    10021 cgggctgggg caatgtcgcc tttaccggcg gcagagacat tccttacttc ctggtgccgc
    10081 ttttcggccc tatcgttggc gcgattgtag gtgcatttgc ctaccgcaaa ctgattggtc
    10141 gccatttgcc ttgcgatatc tgtgttgtgg aagaaaagga aaccacaact ccttcagaac
    10201 aaaaagcttc gctgtaatat gactacggga caattaaaca tgactgaaaa aaaatatatc
    10261 gttgcgctcg accagggcac caccagctcc cgcgcggtcg taatggatca cgatgccaat
    10321 atcattagcg tgtcgcagcg cgaatttgag caaatctacc caaaaccagg ttgggtagaa
    10381 cacgacccaa tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa
    10441 gccgatatca gttccgatca aattgcagct atcggtatta cgaaccagcg tgaaaccact
    10501 attgtctggg aaaaagaaac cggcaagcct atctataacg ccattgtctg gcagtgccgt
    10561 cgtaccgcag aaatctgcga gcatttaaaa cgtgacggtt tagaagatta tatccgcagc
    10621 aataccggtc tggtgattga cccgtacttt tctggcacca aagtgaagtg gatcctcgac
    10681 catgtggaag gctctcgcga gcgtgcacgt cgtggtgaat tgctgtttgg tacggttgat
    10741 acgtggctta tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc
    10801 tctcgtacca tgttgttcaa catccatacc ctggactggg acgacaaaat gctggaagtg
    10861 ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt atacggtcag
    10921 actaacattg gcggcaaagg cggcacgcgt attccaatct ccgggatcgc cggtgaccag
    10981 caggccgcgc tgtttggtca gttgtgcgtg aaagaaggga tggcgaagaa cacctatggc
    11041 actggctgct ttatgctgat gaacactggc gagaaagcgg tgaaatcaga aaacggcctg
    11101 ctgaccacca tcgcctgcgg cccgactggc gaagtgaact atgcgttgga aggtgcggtg
    11161 tttatggcag gcgcatccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcc
    11221 tacgattccg aatatttcgc caccaaagtg caaaacacca atggtgtgta tgtggttccg
    11281 gcatttaccg ggctgggtgc gccgtactgg gacccgtatg cgcgcggggc gattttcggt
    11341 ctgactcgtg gggtgaacgc taaccacatt atacgcgcga cgctggagtc tattgcttat
    11401 cagacgcgtg acgtgctgga agcgatgcag gccgactctg gtatccgtct gcacgccctg
    11461 cgcgtggatg gtggcgcagt agcaaacaat ttcctgatgc agttccagtc cgatattctc
    11521 ggcacccgcg ttgagcgccc ggaagtgcgc gaagtcaccg cattgggtgc ggcctatctc
    11581 gcaggcctgg cggttggctt ctggcagaat ctcgacgagc tgcaagagaa agcggtgatt
    11641 gagcgcgagt tccgtccggg catcgaaacc actgagcgta attaccgtta cgcaggctgg
    11701 aaaaaagcgg tcaaacgcgc gatggcgtgg gaagaacacg acgagtaatg taaatgccgg
    11761 atgaagcgtt tatgccgcat ccggtagtcc tgaaacgtgc gggggcaacc ccgcacacat
    11821 caataatccc acccttcccc tgtgctacac ttcgcgccat tccttactgc ttagagtttg
    11881 ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg gcgctggctg
    11941 gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg gtaaacgcca
    12001 tgcgtattat gctcaaccag gtcaacattg acggcaccat cgtcattggt gaaggtgaaa
    12061 tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc ggcgacgcgg
    12121 tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc caggctaacg
    12181 cgctggcggt gctggcagta ggcgataaag gctgcttcct caatgcgccg gatatgtata
    12241 tggagaagct gattgtcggg ccgggagcca aaggcaccat tgatctgaac ctgccgctgg
    12301 cggataacct gcgcaatgta gcggcggcgc tcggcaaacc gttgagcgaa ctgacggtaa
    12361 cgattctggc taaaccacgc cacgatgccg ttatcgctga aatgcagcaa ctcggcgtac
    12421 gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt atgccagaca
    12481 gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt tctgcggcgg
    12541 tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat gacgtcaaag
    12601 gcgacaacga agagaatcgt cgcattggcg agcaggagct ggcacgctgc aaagcgatgg
    12661 gcatcgaagc cggtaaagta ttgcgtctgg gcgatatggc gcgcagcgat aacgtcatct
    12721 tctctgccac cggtattacc aaaggcgatc tgctggaagg tattagccgc aaaggcaata
    12781 tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc cgcattcagt
    12841 ccatccacta tctggatcgc aaagacccgg aaatgcaggt gcacattctc tgagtgattt
    12901 gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag aacgatgata
    12961 aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc aaagtcacta
    13021 aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc gtgcttccgt
    13081 ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc gtccagcgcg
    13141 cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg gtcaccgtcc
    13201 ccgatggcaa attaagccca cgattggcgg cactgaaacc gggcgatgaa gtgcaggtgg
    13261 ttagcgaagc agctggcttc tttgttctgg atgaagtacc ggactgcgaa acgctatgga
    13321 tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta ggcaaagatt
    13381 tagatcgctt caaaaatctg gtcctggttc acgccgcacg ttatgccgcc gacttaagct
    13441 atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc attcagacgg
    13501 tggtcagtcg ggaaacggca gcggggtcgc tcaccgggcg gataccggcg ttaattgaaa
    13561 gtggggaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc catgtgatgc
    13621 tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag acccggcaga
    13681 tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac tggtaagcgg
    13741 ttacttatcg ataaacggca cgatgagcaa atccgcactc atcttattga tcatcccgcg
    13801 atatgccggc atcaaacggt tgataaatga gtgatgatga ccacagacaa ggaggtcgca
    13861 ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt caatacgcag
    13921 ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat ccgacttatt
    13981 cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat acaactcgct
    14041 taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg cgagctccag
    14101 agctttattc accagtaagg catcttcttc attcccggaa attgccacgc caatgtgttt
    14161 ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg cagtgataac
    14221 tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag ctcaacttgc
    14281 ccctccagat ccttaatctt gtaggccacg ctggcccctt catctgcaga ggatgcagcg
    14341 gcggcaagct ctttcagttt ggtcataaaa gactctgctt cttcgcgggt ggcaaaaaag
    14401 cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc aatcgcacat
    14461 cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat attcgcctct
    14521 ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa gtaatgatct
    14581 tatagattac catttttttt gatattgctt aaggtgaaga gttcagaagg attttccggg
    14641 tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta agagatatat
    14701 taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctggg tatctttacc
    14761 atatttattt tcgccttgtg tgccaatgaa cgctccaaga tcgataagca tcatcaccag
    14821 aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta aaatcgccca
    14881 gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc cggaacgccc
    14941 gcgatcatgc aagcgcttaa cggttactgc cgctgttggc caaagcaagc acacaaggca
    15001 aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa gcaccagcat
    15061 gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac gccctttaaa
    15121 tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt ttccaggatt
    15181 ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat aacaaaaggt
    15241 actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat taaccgttac
    15301 aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc tgctggctgg
    15361 agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca gccagaatcc
    15421 cagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag ccaatctcac
    15481 tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg agcgcggtac
    15541 cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag agcaaaaagc
    15601 agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca ccccattaat
    15661 gaccaaaaca caaagccaga aaaaactgca gtcgctgcta acggcgggga aaaacaaacg
    15721 ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaacg gcgaaaaggg
    15781 gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg aaggtttgaa
    15841 taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc acagacctgc
    15901 tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat taaaatgcga
    15961 catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt tcacgagctg
    16021 gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc aatcgcacca
    16081 ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat catgctgggt
    16141 gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc tgccgctatg
    16201 ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg tacttaccac
    16261 aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca gggcctgact
    16321 ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac tgaagaagtt
    16381 tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt cgaaggtgcg
    16441 gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac tccggctcag
    16501 gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc taacatcgct
    16561 gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc agaactgttt
    16621 gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc tgacgctttc
    16681 gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca ggtgccggat
    16741 ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct cacactgatg
    16801 atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt gtgtcgccag
    16861 caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc ccatatgttc
    16921 ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta ccagttcgct
    16981 ttccgttacc cgacgtgcca ggtattcatg cccgcgcaaa ccacctggca gtggcaacca
    17041 gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag gacgaataca
    17101 agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga tcgccaaaga
    17161 aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac gcgcctgcca
    17221 ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt cagtaccgtt
    17281 aatacgatac gttggcatca acagatattg cagcggacca ttaagatctt ttaaaaccac
    17341 gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt gctgctgatt
    17401 gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac cggttaattt
    17461 ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa aaagaagacc
    17521 cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac gcaaaatcgt
    17581 gacaaataaa aaacccggtc gcgagaaacc accgggtttg ataattatcc tggggaaatc
    17641 agcgtttgca gatctgatcg aacgtaccgc cgttagcaaa atgctctttt tgcgctttcg
    17701 tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac gcattttcgt
    17761 actttttcgc cacctcagcg tcgcgcggac ggtagtagtt tttcgcggca atttcctgac
    17821 cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg ccttttttct
    17881 cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca ctcggcgtga
    17941 cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg ttttcccagg
    18001 cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg cgcgcgccag
    18061 aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct tttgcctgat
    18121 cgttgttgtt gtgatgcagc gcgtagcccc aggcagccag gtagttccag cgtgcgccac
    18181 cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga tcgttccagt
    18241 catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa gtgtacggtg
    18301 cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccgcgt tccgcaattg
    18361 cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg ttgattaccg
    18421 acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta tcaccagttt
    18481 gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg cgcgttggat
    18541 catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc gccagcaaaa
    18601 atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg ttgtgatgag
    18661 caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg ctataacttg
    18721 cgggtatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg ctgacaaagt
    18781 gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa agtttgcaaa
    18841 ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg catttttgct
    18901 tctgtcatcg gtttcagggt aaaggaatct gcctttttcc gaaatcatta atacagtttt
    18961 ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat agcgtcgatg
    19021 atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc gtaacctgcc
    19081 agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc gtaaggcacc
    19141 ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg accggtttct
    19201 ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat cgccacgatc
    19261 gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc ttcacggctg
    19321 aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc agccaacgtc
    19381 agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg agaagaagag
    19441 gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa gaaaccgata
    19501 gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat gcacgggaag
    19561 cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgcctat aaccaccagc
    19621 gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt ctcgtcgcgg
    19681 aattccggga agcgcgcaga accgaggaac gtaccgccac ggttgatcat gtcagaaacg
    19741 ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc gtcataaatg
    19801 cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat tgcggcgttc
    19861 atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat gactacctct
    19921 gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat cgaagtgatc
    19981 caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag gtcaggccaa
    20041 atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga ttatgaaagc
    20101 atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat aattacatcc
    20161 gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat atgtgcctga
    20221 accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt cggccctgac
    20281 tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac gatatcaata
    20341 atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac cgcctcatat
    20401 cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa cagagcatcg
    20461 gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc gttcatcata
    20521 acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt ccggcgcacc
    20581 acccaacgct gaaacgagac aaggataatc gtacaaatta gcgccacaat tgtcacgata
    20641 accccgacgc ctggatctgt catcggtgtt ggagatatca gatgttgaat acccgtcaaa
    20701 aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc gagggactct
    20761 gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata tcgcaccacc
    20821 agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc ggcgagaata
    20881 ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcaa cgaagccatc
    20941 gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt cataaatact
    21001 cccgctatca actgacgcta gtataacgga agcaaatcat ctgcaatgca ttaagcagca
    21061 ggcaaattga ggataaaaaa aaaaccccac atcatgtggg ggaagacagg gatggtgtct
    21121 atggcaagga aaacagggtt tactactggg aacgtgagtt gctactactc aatagcttca
    21181 acgatgaact tttttgccat tgcgtcacgt cacgcaactg ctccattcgt tgttgatgtt
    21241 tctcgtttaa aaccgcttgc tgctccggcg ttaacaggcg atacatttgg ttgcggactt
    21301 ttgccatctc aacctgacga gcaatttgct cattcgccat tttttctgcc tgtgcgcgca
    21361 cagcgttttc atcaaaattt tctgcggtga caaggcgatg cattgtctcc agttcgctaa
    21421 cattaacagg aggctgttcg tgccgggcct gttgcataag atctcgcatc tgctgacgct
    21481 gatgttcggt taaacttatg ccgtcgaaca tatggctctg cgtactgcgc tgcgtaagtt
    21541 cttcacccgg atgccagtta tcgcctgaac cgacttcagc agcgtggctt aatgaactga
    21601 ctgccagcgt tgaggccatg acggcagcgg taactatgcg catcatttgc tcccaaaatc
    21661 tttctgtcgc gattcaacga tagagagttt acgattcagg ctgcaaacat gcgtcagggg
    21721 gtgtaaaaca acgtaaagtc atggattagc gacgtctgat gacgtaattt ctgcctcgga
    21781 ggtatttaaa caatgaataa aatcctgtta gttgatgatg accgagagct gacttcccta
    21841 ttaaaggagc tgctcgagat ggaaggcttc aacgtgattg ttgcccacga tggggaacag
    21901 gcgcttgatc ttctggacga cagcattgat ttacttttgc ttgacgtaat gatgccgaag
    21961 aaaaatggta tcgacacatt aaaagcactt cgccagacac accagacgcc tgtcattatg
    22021 ttgacggcgc gcggcagtga acttgatcgc gttctcggcc ttgagctggg cgcagatgac
    22081 tatctcccga aaccgtttaa tgatcgtgag ctggtggcac gtattcgcgc gatcctgcgc
    22141 cgttcgcact ggagcgagca acagcaaaac aacgacaacg gttcaccgac actggaagtt
    22201 gatgccttag tgctgaatcc aggccgtcag gaagccagct tcgacgggca aacgctggag
    22261 ttaaccggta ctgagtttac cctgctctat ttgctggcac agcatctggg tcaggtggtt
    22321 tcccgtgaac atttaagcca ggaagtgttg ggcaaacgcc tgacgccttt cgaccgcgct
    22381 attgatatgc acatttccaa cctgcgtcgt aaactgccgg atcgtaaaga tggtcacccg
    22441 tggtttaaaa ccttgcgtgg tcgcggctat ctgatggttt ctgcttcatg ataggcagct
    22501 taaccgcgcg catcttcgcc atcttctggc tgacgctggc gctggtgttg atgttggttt
    22561 tgatgttacc caagctcgat tcacgccaga tgaccgagct tctggatagc gaacagcgtc
    22621 aggggctgat gattgagcag catgtcgaag cggagctggc gaacgatccg cccaacgatt
    22681 taatgtggtg gcggcgtctg ttccgggcga ttgataagtg ggcaccgcca ggacagcgtt
    22741 tgttattggt gaccaccgaa ggccgcgtga tcggcgctga acgcagcgaa atgcagatca
    22801 ttcgtaactt tattggtcag gccgataacg ccgatcatcc gcagaagaaa aagtatggcc
    22861 gcgtggaact ggtcggtccg ttctccgtgc gtgatggcga agataattac caactttatc
    22921 tgattcgtcc ggccagcagt tctcaatccg atttcattaa cttactgttt gaccgcccgc
    22981 tattactgct gattgtcacc atgttggtca gtacgccgct gctgttgtgg ttggcctgga
    23041 gtctggcaaa accggcgcgt aagctgaaaa acgctgccga tgaagttgcc cagggaaact
    23101 tacgccagca cccggaactg gaagcggggc cacaggaatt ccttgccgca ggtgccagtt
    23161 ttaaccagat ggtcaccgcg ctggagcgca tgatgacctc tcagcagcgt ctgctttctg
    23221 atatctctca cgagctgcgc accccgctga cgcgtctgca actgggtacg gcgttactgc
    23281 gccgtcgtag cggtgaaagc aaggaactcg agcgtattga aaccgaagcg caacgtctgg
    23341 acagcatgat caacgatctg ttggtgatgt cacgtaatca gcaaaaaaac gcgctggtta
    23401 gcgaaaccat caaagccaac cagttgtgga gtgaagtgct ggataacgcg gcgttcgaag
    23461 ccgagcaaat gggcaagtcg ttgacagtta acttcccgcc tgggccgtgg ccgctgtacg
    23521 gcaatccgaa cgccctggaa agtgcgctgg aaaacattgt tcgtaatgct ctgcgttatt
    23581 cccatacgaa gattgaagtg ggctttgcgg tagataaaga cggtatcacc attacggtgg
    23641 acgacgatgg tcctggcgtt agcccggaag atcgcgaaca gattttccgt ccgttctatc
    23701 ggaccgatga agcacgcgat cgtgaatctg gcggtacagg attggggctg gcgattgttg
    23761 aaaccgccat tcagcagcat cgtggctggg tgaaggcaga agacagcccg ctgggcggtt
    23821 tacggctggt gatttggttg ccgctgtata agcggagtta aactccgcat ttgtaggcag
    23881 gataaggcgt ttacgccgca tccggcattt gagcaggatg cctgatgcga cgctgatagc
    23941 gtcttatcag gcctacactc cgggggttat tttccccaca accggcggga aaaatcttcg
    24001 atcttgccgc tcagtcggcg cttttgcatc gtccgcgtcc agcttttcga taacccggcg
    24061 gcggagagta aacggtgata ttgatcgtca tcaaacggca tatgccatgc gatggcggca
    24121 gcctcctgca cggtcacatc gctgacacgg gaaaccaact ccagcggcgc gtccgcagac
    24181 actttccctg gcgctatcac gctatacaac caccccacct tacctgtgtt ttgcatcaac
    24241 tgcgctatat cgctgatatc aaaatggtaa ttgagtttat agcaaggcga gcgcggctgg
    24301 ctgacctgaa ttaatgcctc tccccagcgg aaaatatcgc ccatataaac attactttcc
    24361 gtgagaccgt cggttgagat attttcgcca aacgcaggcg caacaaacaa ctccgcctgt
    24421 tccggaaatt cccgcgccca gtagagataa tgctcacgag gataatgaca cagcgctctg
    24481 tctggtccgc cgtgaacctt tttctccgcc tgctcgtcac cttccagccc cagctccgtc
    24541 agcatcaact caccatcaac ctggattttg gcaattgcgc tgggtttgcc ttcgggataa
    24601 gcctgaatct tgcctgtgta tacatcaacc ggatatcgca tctgttccct ctccacgaga
    24661 aatttcccca ttagaccata tttattttat gtcaggcgtg ctgttttcgc ggtaagtatc
    24721 caggcaggaa ctcaatagca aaccggacga acgttagttc atccggctgt ggggattaag
    24781 gaagcaggtt tatttcaccg tacctaaaat ttcgattttc gctgctctgg ctttgacttt
    24841 acgtgaccag atagaggtaa gaattggcac cagaatcgaa gtcacaatga ccgccgtcgc
    24901 taccagcgaa gttgctgccg gagccatcgg tttaaacgca ggcaccattt ctgcaatcag
    24961 cacaggtgtc gctaccgcgg cccctgcgga actggaagcg gcaattccgg cagtgccatc
    25021 gccaccgcca atcaatttat cggcgataat caacggaata ccggtcacga taattactgc
    25081 cacacccaac aggatcccca gcaaaccagt ctgggcaatc acagtcaaat caatggtatt
    25141 gcccagcgcg aaggcaaaga atggaatcag cgtttgcacc gctttgctga aaaattctcg
    25201 taattcaggg tcaaggttcc ccagggcaaa gcccaccagg aacggtaata ctgcgccgac
    25261 gaaaacatgc ggttcaaacg aggcaatccc tgcagtgccc agaataatca tcgtcatgag
    25321 cggcccggac tccaacgaca tcaacacaaa tgccccagct tcttcttttg tgccgtactg
    25381 ctgcatgatg gaagcgtaaa gtccgccatt ggtcatatcc atcgccgcca ccagcgccag
    25441 cgttgaaagt ccggcaaaga atccaacttc aacaccatgt tccggaataa tgcgcgaggc
    25501 aattgccgca accacccacg cgacggcaat ttttgttacc accagcgtac cggatttacg
    25561 caataccgtt cccgtcgcgc ttaattttat tgacgccccc atgcaaaaaa accacaccgc
    25621 cagaatgggc accgtaccgg taatcatccc gttggtgaat gatccaaaat atttccccgc
    25681 ccccggcgag aaggtgtggc acagtgcgcc aaggaatagc gggacgagca tcatcccccc
    25741 cgggattttc tcaatcgagc gttttatctg catttccatc acctgtcggc atatttttaa
    25801 gggctgatgc gtcatttctc aatggagcat cagtgttgat ggaaacgaag atagacattc
    25861 atttaatcaa taaaagtgat caaaaccaca tcaattgaaa cactgtttta tttttatcgg
    25921 atcattgttt ttattttgaa attgatcaca aaaaaaccac cgccgttggc gatggttcat
    25981 tttttaagct gatatgcggc ctatacgcct cattgcagca ggcggcaaat gattattttt
    26041 tcgccgcaaa acgtgccgct gcttcgtccc agttcaccac gttccagaac tctttaatgt
    26101 agtccggacg gcggttctgg aatttcaggt agtaagcatg ttcccacaca tccaggccca
    26161 gaatcgggaa gccggaagcg ccagaaatag cttcacccat cagcggagaa tcctggttag
    26221 cagtagaaac caccgccagt ttatcgcctt tcagcaccag ccatgcccag ccggaaccaa
    26281 agcgggaagc tgccgctttt tcaaattctg ctttgaagtt atcaacggag ccgaagtcac
    26341 gttcgatagc cgctttcagg tcaccctgca gggtggtgcc ttttttcaga cctttccaga
    26401 acaggctgtg gttagcgtga ccgccagcgt tgttgcgcag tacggttttc ttgtctgctg
    26461 gcagctggtc cagtttggtg atcagctctt caaccggcag gttggcaaat tctggcaggc
    26521 tttccagcgc cgcgttggcg ttgtttacgt aggtctgatg gtgtttggtg tggtggattt
    26581 ccatggtctg cttatcgaag tgcggttcca gggcatcgta agcatacggc agggatggca
    26641 gggtatagct catattcatc tccagtattg tcgggcggcc gattgttaat gccgcgtaag
    26701 cagttggttc attatagtta attaaatgat attgaaaatg attatcaatg ccgtactttt
    26761 cgtaagggta tggttttgca ggaaaatgcc cgagatgtga agcaaatcac ccacttaatg
    26821 ccgtgattgc cagtaaatcg acaacggcgg caacaggcga aaggttaatc gacagcacga
    26881 tttttacact catctcgtcg gagatgtgaa gcgacgaaaa atgatgagga taagaagatg
    26941 agtaacgcga ttacgatggg gatattttgg catttgatcg gcgcggccag tgcagcctgt
    27001 ttttacgctc cgttcaaaaa agtaaaaaaa tggtcatggg aaaccatgtg gtcagtcggt
    27061 gggattgttt cgtggattat tctgccgtgg gccatcagcg ccctgttact accggatttc
    27121 tgggcgtatt acagctcgtt tagtctctct acgctgctgc ctgtttttct gttcggcgct
    27181 atgtggggga tcggtaatat caactatggc ctgaccatgc gttatctcgg catgtcgatg
    27241 ggaattggca tcgccattgg cattacgttg attgtcggta cgctgatgac gccaattatc
    27301 aacggcaatt tcgatgtgct gattaatacc gaaggcggac gcatgacgtt gctcggcgtt
    27361 ctggtggcgc tgattggcgt agggattgta actcgagccg ggcagttgaa agagcgcaag
    27421 atgggcatta aagccgaaga gttcaatctg aaaaaagggc tggtgctggc ggtgatgtgc
    27481 ggcattttct ctgccgggat gtcctttgcg atgaacgccg caaaaccgat gcatgaagcc
    27541 gctgccgcac taggcgtcga tccactgtat gtcgctctgc caagctatgt catcatcatg
    27601 ggcggcggcg cgatcatcaa cctcggtttc tgttttattc gtctggcaaa agtgaaggat
    27661 ttgtcgctaa aagccgactt ctcgctggca aaaccgctga tcactcacaa tgtgttactc
    27721 tcagcactgg gcgggttgat gtggtatctg caattctttt tctatgcctg gggccacgcc
    27781 cgcattccgg cgcaatatga ctacatcagt tggatgctgc atatgagctt ctatgtattg
    27841 tgcggcggta tcgtcgggct ggtgctgaaa gagtggaaca atgcaggacg ccgtccggta
    27901 acggtgttga gcctcggttg tgtggtgatt attgtcgccg ctaacatcgt cggcatcggc
    27961 atggcgaatt aatctttctg cgaattgaga tgacgccact ggctgggcgt catcccggtt
    28021 tcccgggtaa acaccaccga aaaatagtta ctatcttcaa agccacattc ggtcgaaata
    28081 tcactgatta acaggcggct atgctggaga agatattgcg catgacacac tctgacctgt
    28141 cgcagatatt gattgatggt cattccagtc tgctggcgaa attgctgacg caaaacgcgc
    28201 tcactgcacg atgcctcatc acaaaattta tccagcgcaa agggactttt caggctagcc
    28261 gccagccggg taatcagctt atccagcaac gtttcgctgg atgttggcgg caacgaatca
    28321 ctggtgtaac gatggcgatt cagcaacatc accaactgcc cgaacagcaa ctcagccatt
    28381 tcgttagcaa acgacacatg ctgactactt tcatgctcaa gctgaccgat aacctgccgc
    28441 gcctgcgcca tccccatgct acctaagcgc cagtgtggtt gccctgcgct ggcgttaaat
    28501 cccggaatcg ccccctgcca gtcaagattc agcttcagac gctccgggca ataaataata
    28561 ttctgcaaaa ccagatcgtt aacggaagcg taggagtgtt tatcgtcagc atgaatgtaa
    28621 aagagatcgc cacgggtaat gcgataaggg cgatcgttga gtacatgcag gccattaccg
    28681 cgccagacaa tcaccagctc acaaaaatca tgtgtatgtt cagcaaagac atcttgcgga
    28741 taacggtcag ccacagcgac tgcctgctgg tcgctggcaa aaaaatcatc tttgagaagt
    28801 tttaactgat gcgccaccgt ggctacctcg gccagagaac gaagttgatt attcgcaata
    28861 tggcgtacaa atacgttgag aagattcgcg ttattgcaga aaaccatccc gtccctgacg
    28921 aatatcacgc ggtgaccagt taaactctcg gcgaaaaagc gtcgaaaagt ggttactgtc
    28981 gctgaatcca cagcgatagg cgatgtcagt aacgctggcc tcgctgtggc gtagcagatg
    29041 tcgggctttc atcagtcgca ggcggttcag gtatcgctga ggcgtcaatc ccgtttgctg
    29101 cttaagctgc cgatgtagcg tacgcagtga aagagaaaat tgatccgcca cggcatccca
    29161 attcacctca tcggcaaaat ggtcctccag ccaggccaga agcaagttga gacgtgatgc
    29221 gctgttttcc aggttctcct gcaaactgct tttacgcagc aagagcagta attgcataaa
    29281 caagatctcg cgactggcgg tcgagggtaa atcattttcc ccttcctgct gttccatctg
    29341 tgcaaccagc tgtcgcacct gctgcaatac gctgtggtta acgcgccagt gagacggata
    29401 ctgcccatcc agctcttgtg gcagcaactg attcagcccg gcgagaaact gaaatcgatc
    29461 cggcgagcga tatagcacat tggtcagaca cagattatcg gtatgttcat acagatgccg
    29521 atcatggtcg cgcacaaaac agaccgtgcc accggtgatg gtatagggct gcccattaaa
    29581 cacatgaata cccgtgccat gttcgacaat cacaatttca tgaaaatcat gatgatgttc
    29641 aggaaaatcc gcctgcggga gccggggttc tatcgccacg gacgcgttac cagacggaaa
    29701 aaaatccaca ctatgtaata cggtcatact ggcctcctga tgtcgtcaac acggcgaaat
    29761 agtaatcacg aggtcaggtt cttaccttaa attttcgacg ggaaaccacg taaaaaacgt
    29821 cgatttttca agatacagca tgaattttca tgaaatgcgg tgagcatcac accaccacaa
    29881 ttcagcaatt tgtgaacatc atcacgttca tctttccctg gttgccaatg gcccattttc
    29941 ctgtcagtag cgagaaggtc gcgaattcag gcgcttttta gactggtcgt aatgaaattc
    30001 agcaggatca cattatgacc tttcgcaatt gtgtcgccgt cgatctcggc gcatccagtg
    30061 ggcgcgtgat gctggcgcgt tacgagcgtg aatgccgcag cctgacgctg cgcgaaatcc
    30121 atcgttttaa aaatgggctg catagccaga acggttatgt cacctgggat gtggatagcc
    30181 tggaaagtgc cattcgcctt ggattaaaca tggtgtgtga ggaagggatt cgtatcgata
    30241 gcattgggat tgatacctgg ggcgtggact ttgtgctgct cgaccaacag ggtcagcgtg
    30301 tgggcctgcc cgttgcttat cgcgatagcc gcaccaatgg cctaatggcg caggcacagc
    30361 aacaactcgg caaacgcgat atttatcaac gtagcggcat ccagtttctg cccttcaata
    30421 cgctttatca gttgcgtgcg ctgacggagc aacaacctga acttattcca cacattgctc
    30481 acgctctgct gatgccggat tacttcagtt atcgcctgac cggcaagatg aactgggagt
    30541 acaccaatgc caccaccaca caactggtca atatcaatag cgacgactgg gacgagtcgc
    30601 tactggcgtg gagcggggcc aacaaagcct ggtttggtcg cccgacgcat ccgggtaatg
    30661 tcattgggca ctggatttgc ccgcagggta atgagattcc ggtggtcgcc gttgccagcc
    30721 atgataccgc cagcgcggtt atcgcctcgc cgttaaacgg ttcacgcgcc gcttatctct
    30781 cttctggcac ctggtcattg atgggcttcg aaagccagac gccatttacc aatgacacgg
    30841 cgctggcagc caacatcacc aatgaaggcg gggcggaagg tcgctatcgg gtgctgaaaa
    30901 atattatggg cttatggctg cttcagcgag tgcttcagga acggcaaatc aacgatctcc
    30961 cggcgcttat cgccgcgaca caggcacttc cggcctgccg cttcatcatc aatcccaatg
    31021 acgatcgctt tattaatcct gaggcgatgt gcagcgaaat tcaggctgcg tgtcgggaaa
    31081 cggcgcaacc gatcccggaa agtgatgctg aactggcgcg ctgcattttc gacagtctgg
    31141 cgctgctgta tgccgatgtg ttgcatgagc tggcgcagct acgcggtgaa gatttctcgc
    31201 aactgcatat tgtcggcggc ggctgccaga acacgctgat caaccagcta tgcgccgatg
    31261 cctgcggtat tcgggtgatc gccgggcctg ttgaagcctc gacgctcggc aatatcggca
    31321 tccagttaat gacgctggat gaactcaaca atgtggatga ttttcgtcaa gtcgtaagca
    31381 ccaccgcgaa tctgaccacc tttaccccta atcctgacag tgaaattgcc cactatgtgg
    31441 cgcagattca ctctacacga cagacaaagg agctttgcgc atgaccactc aactggaaca
    31501 ggcctgggag ctggcgaaac agcgtttcgc ggcggtgggg attgatgtcg aggaggcgct
    31561 gcgccaactt gatcgtttac ccgtttcaat gcactgctgg cagggcgatg atgtttccgg
    31621 ttttgaaaac ccggaaggtt cgctgaccgg tgggattcaa gctactggta attatccggg
    31681 caaagcgcgt aatgccagtg agctacgtac cgatctggaa caggctatgc ggctgattcc
    31741 ggggccgaaa cggcttaatt tacatgccat ctatctggaa tcagacacgc ctgtctcgcg
    31801 cgatcagatc aaaccagagc acttcaaaaa ctgggttgaa tgggcgaaag ccaatcagct
    31861 cggtctggat tttaacccct cctgtttttc gcatccgcta agcgccgatg gctttacgct
    31921 ttcccatgcc gacgacagca ttcgccagtt ctggattgat cactgcaaag ccagccgtcg
    31981 cgtttcggcc tattttggcg agcaactcgg cacaccatcg gtgatgaaca tctggatccc
    32041 ggatggtatg aaagatatca ccgttgaccg tctcgccccg cgtcagcgtc tgctggcagc
    32101 actggatgag gtgatcagcg agaagctaaa ccctgcgcac catatcgacg ccgttgagag
    32161 caaattgttt ggcattggcg cggagagcta cacggttggc tccaatgagt tttacctggg
    32221 gtatgccacc agccgccaga cggcgctgtg cctggacgcc gggcacttcc acccgactga
    32281 agtgatttcc gacaagattt ccgccgccat gctgtatgtg ccgcagttgc tgctgcacgt
    32341 cagtcgtccg gttcgctggg acagcgatca cgtagtgctg ctggatgatg aaacccaggc
    32401 aattgccagt gagattgtgc gtcacgatct gtttgaccgg gtgcatatcg gccttgactt
    32461 cttcgatgcc tctatcaacc gcattgccgc gtgggtcatt ggtacacgca atatgaaaaa
    32521 agccctgctg cgtgcgttgc tggaacctac cgccgagctg cgcaagttgg aagcggcggg
    32581 cgattacact gcgcgtctgg cactgctgga agagcagaaa tcgttgccgt ggcaggcggt
    32641 ctgggaaatg tattgccaac gtcacgatac cccaacgggt agcgaatggc tggagagcgt
    32701 gcgggcttat gagaaagcga ttttgagcca gcgtgggtaa acactgccgg atgcggcacg
    32761 agcgccttat ccggcctacg ggtcgaatgt ccatgcgggc tgtcaccctc accccggccc
    32821 tctccctgaa agggcgaggg agaaaaacat agcctcgtgc ggcaacggag ctgaacactg
    32881 ctccggacag tcccctcgcc cctacgggga gagggttagg gtgaagggaa caagcagcac
    32941 catgtctaaa caaggcaacc taatttacag gaacacagaa catgcaaaac attacccagt
    33001 cctggtttgt ccagggaatg atcaaagcca ccaccgatgc ctggctgaaa ggctgggacg
    33061 agcgcaacgg cggtaacctg acgctgcgcc tggatgacgc cgatatcgcg ccttataaag
    33121 acaacttcca tgcacaaccg cgttatatcc cgctcagcca acccatgcct ttactggcaa
    33181 atacaccgtt tattgtcacc ggctccggca aattcttccg taacgtccag cttgatcctg
    33241 cagctaactt aggcgtcgta aaagtcgaca gcgacggcgc gggctaccat attctctggg
    33301 ggttaaccaa cgaagccgtt ccaacttctg aacttccggc tcactttctc tcccactgcg
    33361 agcgcattaa agccaccaac ggtaaagatc gggtgatcat gcactgccac gccaccaacc
    33421 tgatcgccct cacctatgta ctggaaaacg acaccgcggt ctttactcgc caactgtggg
    33481 aaggcagcac cgagtgtctg gtggtattcc cggatggcgt tggcattctg ccgtggatgg
    33541 tgcccggcac ggacgaaatc ggccaggcga ccgcacaaga gatgcaaaaa cattcgctgg
    33601 tgttgtggcc cttccacggc gtcttcggca gcgggtcgac gttggatgaa acattcggtt
    33661 taatcgacac cgcagaaaaa tcagcacaag tattagtgaa ggtttattcg atgggcggca
    33721 tgaaacagac catcagccgt gaagagttga tagcgctcgg caagcgcttc ggcgttacac
    33781 cactagccag tgcgctggcg ctgtaaggag caaacatgat ccgcaaagcc tttgtcatgc
    33841 aggttaaccc cgacgcccac gaagagtatc agcgtcggca taaccccatc tggccggaac
    33901 tggaagcagt gttgaaatct cacggtgcgc ataactacgc catttacctc gacaaagcgc
    33961 gtaatctgct gtttgccacg gtagagattg aatctgaaga acggtggaat gcggtagcca
    34021 gtactgatgt ttgccaacgt tggtggaaat atatgaccga tgttatgccc gctaacccgg
    34081 ataacagccc ggtgagtagc gagctgcaag aagtgtttta cctgccgtaa ttgcccaatg
    34141 tgccggagat gcctccggca caatatttta aatttcgatt tgaaaaatta aacatatta
//
LOCUS       DAZKIV010000028        34192 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZKIV010000028 DAZKIV010000000
VERSION     DAZKIV010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.028"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            37..777
                     /locus_tag="ACHOT6_001459"
     CDS             37..777
                     /locus_tag="ACHOT6_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9946701.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIAEIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            812..3412
                     /gene="htrE"
                     /locus_tag="ACHOT6_001460"
     CDS             812..3412
                     /gene="htrE"
                     /locus_tag="ACHOT6_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFP9946702.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            3429..3998
                     /locus_tag="ACHOT6_001461"
     CDS             3429..3998
                     /locus_tag="ACHOT6_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9946703.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRITKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            4013..4615
                     /gene="yadL"
                     /locus_tag="ACHOT6_001462"
     CDS             4013..4615
                     /gene="yadL"
                     /locus_tag="ACHOT6_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFP9946704.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            4642..5238
                     /locus_tag="ACHOT6_001463"
     CDS             4642..5238
                     /locus_tag="ACHOT6_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9946705.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFVNEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDILSGQVMSHILVDVYYE"
     gene            5288..6544
                     /locus_tag="ACHOT6_001464"
     CDS             5288..6544
                     /locus_tag="ACHOT6_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFP9946706.1"
                     /translation="MMKTFFKYFLFLVLSCSCFTAIGNPTSFLVGNNAGVGDGNFKGP
                     STAAQVTFRYASTTNNLVFYKPTQLGPTGVKLRWEQLDTASGGGFLYCNASDRANPDG
                     LMDIENAMVDSGKTYGGHKLFKTSVPGLYYTLFIAKLWSAYSTKTTISDSGLYVGDTT
                     PQKFQWIITDPNLQHDGCDNAIWYDRFWAIGGVVHDLTIEFYTDTDFNPTTNQQVSLS
                     SSSTYLYAFKAYSPGTRVVDHSHHLYIDFDLLNVKLTNPTCFTAVLTGKSVSGSTVKM
                     GEYAPGQIKNGATPVPFDISLENCVRVGDIETKLSSGKLGTENKQLLGNTITGSDAAK
                     GVGVLIEGLANSKSALMILKPNDSASVYKDNTGQTQNNDSDAIYPEDQGITYPLHFQA
                     TLKQDGNIAIEPGEFKATSTFQVTYP"
     gene            6657..7451
                     /gene="panB"
                     /locus_tag="ACHOT6_001465"
     CDS             6657..7451
                     /gene="panB"
                     /locus_tag="ACHOT6_001465"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFP9946707.1"
                     /translation="MKPTTISLLQKYKQEKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVQMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            7463..8314
                     /gene="panC"
                     /locus_tag="ACHOT6_001466"
     CDS             7463..8314
                     /gene="panC"
                     /locus_tag="ACHOT6_001466"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFP9946708.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(8592..9494)
                     /gene="rpnC"
                     /locus_tag="ACHOT6_001467"
     CDS             complement(8592..9494)
                     /gene="rpnC"
                     /locus_tag="ACHOT6_001467"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000339961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFP9946709.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPQVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVVRDRETGGMSMMTLAQWFEEKGIEKGIQQGRQEVSQEFAQRLLSKG
                     MSREDVAEMTNLSLAEIDKLINSN"
     gene            9768..10148
                     /gene="panD"
                     /locus_tag="ACHOT6_001468"
     CDS             9768..10148
                     /gene="panD"
                     /locus_tag="ACHOT6_001468"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFP9946710.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIANFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10152..11381)
                     /locus_tag="ACHOT6_001469"
     CDS             complement(10152..11381)
                     /locus_tag="ACHOT6_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9946711.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYVFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFIVTSRIKRHPQKW
                     DPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(11445..11885)
                     /locus_tag="ACHOT6_001470"
     CDS             complement(11445..11885)
                     /locus_tag="ACHOT6_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9946712.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGELLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(11990..12760)
                     /locus_tag="ACHOT6_001471"
     CDS             complement(11990..12760)
                     /locus_tag="ACHOT6_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946713.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(12757..13683)
                     /locus_tag="ACHOT6_001472"
     CDS             complement(12757..13683)
                     /locus_tag="ACHOT6_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9946714.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFSQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            13792..14454
                     /gene="can"
                     /locus_tag="ACHOT6_001473"
     CDS             13792..14454
                     /gene="can"
                     /locus_tag="ACHOT6_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFP9946715.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(14495..15031)
                     /gene="hpt"
                     /locus_tag="ACHOT6_001474"
     CDS             complement(14495..15031)
                     /gene="hpt"
                     /locus_tag="ACHOT6_001474"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFP9946716.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            15237..17627
                     /gene="gcd"
                     /locus_tag="ACHOT6_001475"
     CDS             15237..17627
                     /gene="gcd"
                     /locus_tag="ACHOT6_001475"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFP9946717.1"
                     /translation="MAINNTGSRRLLITLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVGEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(17674..19224)
                     /gene="cueO"
                     /locus_tag="ACHOT6_001476"
     CDS             complement(17674..19224)
                     /gene="cueO"
                     /locus_tag="ACHOT6_001476"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFP9946718.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVNELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            19390..19737
                     /locus_tag="ACHOT6_001477"
     CDS             19390..19737
                     /locus_tag="ACHOT6_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFP9946719.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            19843..20709
                     /gene="speE"
                     /locus_tag="ACHOT6_001478"
     CDS             19843..20709
                     /gene="speE"
                     /locus_tag="ACHOT6_001478"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFP9946720.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAVHTAAFALP
                     QYLQDALASQPS"
     gene            20725..21519
                     /gene="speD"
                     /locus_tag="ACHOT6_001479"
     CDS             20725..21519
                     /gene="speD"
                     /locus_tag="ACHOT6_001479"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFP9946721.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(21556..21918)
                     /gene="yacL"
                     /locus_tag="ACHOT6_001480"
     CDS             complement(21556..21918)
                     /gene="yacL"
                     /locus_tag="ACHOT6_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFP9946722.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22093..24690)
                     /gene="acnB"
                     /locus_tag="ACHOT6_001481"
     CDS             complement(22093..24690)
                     /gene="acnB"
                     /locus_tag="ACHOT6_001481"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFP9946723.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25045..26802
                     /locus_tag="ACHOT6_001482"
     CDS             25045..26802
                     /locus_tag="ACHOT6_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFP9946724.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGLGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGCHRDSNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNETEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(26873..28297)
                     /gene="lpdA"
                     /locus_tag="ACHOT6_001483"
     CDS             complement(26873..28297)
                     /gene="lpdA"
                     /locus_tag="ACHOT6_001483"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFP9946725.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(28505..30397)
                     /gene="aceF"
                     /locus_tag="ACHOT6_001484"
     CDS             complement(28505..30397)
                     /gene="aceF"
                     /locus_tag="ACHOT6_001484"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFP9946726.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(30412..33075)
                     /gene="aceE"
                     /locus_tag="ACHOT6_001485"
     CDS             complement(30412..33075)
                     /gene="aceE"
                     /locus_tag="ACHOT6_001485"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFP9946727.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELAREGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(33236..34000)
                     /gene="pdhR"
                     /locus_tag="ACHOT6_001486"
     CDS             complement(33236..34000)
                     /gene="pdhR"
                     /locus_tag="ACHOT6_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFP9946728.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
BASE COUNT         8559 a         8917 c         8235 g         8481 t
ORIGIN      
        1 attccctgtt ttaattaact ctgtcaggga tggtttatgt tttttaacac caaacataca
       61 acagctttat gcttcgtaac ctgtatggct tttagttcat cgtctattgc ggagattgtc
      121 atttcgggta ctcgcgtaat atataaaagc gatcaaaaaa gtgtcaacgt acgtctggaa
      181 aataaaggga ataacccgtt gcttgtccag agttggttag atactggcga tgacaacgct
      241 gagcctggca gtattacagt cccttttact gccacgccgc cagtatcgcg tattgatgcc
      301 aaacgtgggc aaacaatcaa attaatgtac acagccagca cctcactgcc taaagacaga
      361 gagagcgtgt tctggtttaa cgtactggaa gttccaccaa aaccagatgc agaaaaggtc
      421 gcgaatcaaa gtctgctgca actggcattt cgcacacgta taaaactttt ctatcgcccg
      481 gatggattga agggaaatcc ctctgaagcc ccgttagccc tgaagtggtt ctggtcaggt
      541 tcagaaggta aggcgtcatt gcgagtgacc aatccaaccc cttactacgt ctcttttagc
      601 agtggtgatt tagaagctag cggtaaacgc tatccgattg atgtgaaaat gattgcacca
      661 tttagtgatg aggtcatgaa agtcactggc cttaatggca aagcaagttc tgcaaaagta
      721 catttttacg ccattaatga ttttggtggc gccattgaag gtaatgccag tctgtaatca
      781 ggcaaggata taattccgca ggaagcataa cgtgactata aaaccaacta aaaattatca
      841 taatcatctg acccgtatcg ctacgttttg cgcgctgctg tattgcaata gtgctttctg
      901 tgctgaactt gttgaatatg accatacctt cctgatgggg cagaatgcct caaatattga
      961 tctcagccgg tatagtgaag gtaaccctgc tataccgggt atgtacgacg tcagtgttta
     1021 tgtcaacgat caacctatta ttaaccaaag cattacattt attgaaattg aaggaaaaaa
     1081 aaacgcacaa gcctgtatca cacttaagaa tttattgcag tttcatataa attctcccga
     1141 tataaataac gaaaaagccg ttctgcttgc cagggatgaa acactcggca attgccttaa
     1201 tttgacggaa attatccctc aggcttctgt tcgttatgac gttaacgagc aacgtctgga
     1261 tatagacgtt cctcaggcat gggtaatgaa aaattaccaa aactatgttg atccatcgtt
     1321 atgggaaaat ggcattaatg cggccatgtt gtcatacaac ctcaacggat atcatagtga
     1381 aactcctggt cgaagaaatg acagcattta tgctgctttt aacggtggga tgaatttagg
     1441 tgcatggcga ctgcgtgcct caggcaacta caactggatg accgattctg gcagtaatta
     1501 tgattttaag aatcggtata tacagcgtga tatcgcctcg ctgcgttctc aactcattct
     1561 tggtgagtct tatacaacgg gcgaaacctt tgattctgtc agtatccgcg gcattcgttt
     1621 atacagcgac agccgcatgt tgcctccgac attagcgagt tttgcgccta tcattcatgg
     1681 cgttgctaat accaacgcca aagtcactat tacgcaaggt ggctataaga tttatgaaac
     1741 gactgtgccg ccaggcgctt tcgtcattga tgatctgagt ccgtcaggtt acggcagcga
     1801 tcttattgtt actgtcgaag aatccgatgg ctcaaagcgg acattctcgc aacctttttc
     1861 atcggtcgtt caaatgttac gccctggcgt tggacgttgg gatattagcg gcggccaggt
     1921 cttaaaagat gatattcagg atgagcctaa tttatttcag gccagctact actacggtct
     1981 gaataactat ctaacgggtt atactggtat tcagattacc gataataact ataccgctgg
     2041 tttgttaggt cttggtctga atacttcagt cggtgcattt tctttcgatg tgactcactc
     2101 caatgttcgt ataccggatg ataaaacata tcagggacaa agttatcgtg tttcctggaa
     2161 caagttattc gaagaaacaa gtacttcact gaatatcgcg gcctatcgct attcgacaca
     2221 gaattacctt ggtcttaatg atgcactaac tctaattgat gaagtgaaac atcccgaaca
     2281 agatcttgaa ccgaaatcca tgcgtaatta ctcgcgcatg aaaaaccagg ttacggtcag
     2341 tattaaccaa ccgttgaaat ttgagaaaaa agattacggt tcattttatc tttccggaag
     2401 ttggtccgat tactgggctt cagggcaaaa tcgtagcaat tattctattg gctacagtaa
     2461 cagtgcatcc tggggcagct acagtgtcag tgcacagcgt tcatggaatg aagacggcga
     2521 cactgacgat agcgtttatc tcagtttcac cattcctatt gaaaaattac ttggcactga
     2581 acaacggact tcaggtttcc agagtattga tactcaaatg agcagtgact ttaagggtaa
     2641 taaccaactc aacgtcagta gcagtggcta tagtgataac gctcgcgtca gttatagcgt
     2701 aaatactggc tatacgatga ataaagccag caaagatttg agttatgttg ggggttatgc
     2761 cagctatgaa tcaccgtggg gaagcctggc aggttcagtt tctgcaaata gcgataacag
     2821 tcgtcaagtg tctctcagca ccgacggtgg ttttgtattg catagcggcg gcctgacctt
     2881 cagtaatgac agttttagcg actccgatac actggcggta gttcaggctc caggtgctca
     2941 aggagcgcga ataaattatg gcaacagtac tatcgatcga tggggttatg gtgtcaccag
     3001 cgctctttct ccttatcatg aaaaccgtat cgcgctggat atcaacgatc ttgagaacga
     3061 tgttgaatta aaaagtacca gtgcagtagc tgtaccgcgt cagggttcag tcgtctttgc
     3121 tgattttgaa accgtgcaag ggcaatcagc cattatgaac atcacacgaa gtgatggtaa
     3181 aaatattcca tttgctgcag atatttatga tgagcaaggc aatgtcattg gtaatgttgg
     3241 acagggtgga caagcatttg ttcgtggcat tgagcagcag ggaaatatca gcattaaatg
     3301 gctcgaagaa agtaaacccg taagttgtct tgcgcattat caacaaagtt cagaagcaga
     3361 aaaaatagcg caatctatta ttctgaatgg aattcggtgt cagattcagt aactacaagg
     3421 gacgtcaaat gataaaaata acgccacata aaatagcgat actgatggga atattattat
     3481 caccatcagt atttgcaacg gatattaatg tagagtttac agccactgtc aaagcgacaa
     3541 cctgtaacat cacacttact ggtaataacg tcacgaatga tggcaataat aactacacat
     3601 tgagaatcac taagatgggt ctggataaga tcgcgaataa aacgacagaa tctcaggctg
     3661 attttaaact ggttgctagt gggtgcagca gtggcatcag ttggattgat accactctga
     3721 ccggaaatgc atcatcaagc tcacctaagc ttattatacc gcagtctggt gattcatctt
     3781 cgacgacaag taatatcggt atgggtttca aaaaacggac tactgatgat gccactttcc
     3841 ttaaacctaa cagtgcggaa aagatacgct ggagcacaga cgagatgcag cccgataagg
     3901 gtcttgaaat gaccgttgcg ctacgtgaaa cagatgcagg gcaaggcgta ccggggaatt
     3961 ttcgtgcact ggcaacgttt aatttcatct atcaataaga aggataatta agatgacatt
     4021 taaaaattta cgttatggat tatccagcag tgtcgttttg gctgcctcat tgttcagcgt
     4081 accctcttat gcggcaacag acagcattgg gctgaccgtg attactaccg tagaaatagg
     4141 tacttgtacc gctacattag taaatgactc tgatcaggac atttctgttg ttgactttgg
     4201 cgatgtatat atttctgaaa tcaatgccaa gaccaaagtt aaaacattca aactcaaatt
     4261 caaagactgt gcgggtatcc ccaataaaaa agcacaaata aaattaacca agcgagccac
     4321 atgcgaaggt actgctaatg acggtgcggg gttcgcaaat ggatccacag ccgcagataa
     4381 agcaagtgcc gtcgccgttg aagtctggag caccgaaact ccggcaacag gtagcgcaac
     4441 acaatttagt tgtgtgacac cagcatctca agaggtcact atctccaatg cagccaacgc
     4501 ggttgtttat tatccgatga gtgctcgact ggtcgtcgaa aaaaataaaa ccgtaagcaa
     4561 tgtcactgcg ggtaaatttt ctgcacctgc cacatttaca gtaacctata actaacagac
     4621 tgacagatcc aggaactata tatgcatccc actcaacgta agctgatgaa gagaataatt
     4681 ctgtttctgt cactacagtt tcccattacc tatccagcca tcgctgggca ggatattgac
     4741 cttgttgcca atgtaaaaaa cagcacttgc aaaagcggaa tcagtaacca gggtaatatt
     4801 gatcttggcg tcgttggggt gggatatttt tcagataatg ttactcctga aagctatcaa
     4861 ccaggtggaa aagagttcac tgtcacagta tcagactgtg cattacaggg aactggcgat
     4921 gtgcttaatc agttacatat tgattttaga gcccttagcg gtgtcatggc tgcaggttca
     4981 aggcaaatat ttgtcaatga agtcgcaacc ggggctaaaa atgtcggtgt cgttctcttt
     5041 tccattcagg acccgaccaa tatattcaat gttattagtt cagcaggaaa ttctcgttcc
     5101 gtgtatccag taatgacttc tgccctgcat aattcttcat ggaaatttta tgcccgaatg
     5161 caaaaaatcg atcccgcact agatatccta tctggtcagg taatgagtca tatattagtt
     5221 gatgtgtact acgaataata gatcaaaaca aacgactgct attatgacag cagcgaatgg
     5281 tggggtaatg atgaagacat tcttcaaata ctttcttttt ttggtgctat cctgttcttg
     5341 ctttacagcg attggcaatc ctacaagttt tcttgttggt aacaatgccg gggtaggcga
     5401 tggtaatttc aaaggaccga gcactgcggc tcaggtcact tttagatatg catcaacaac
     5461 caataattta gttttttata aacctacgca acttggcccg acaggtgtta aattgcgctg
     5521 ggaacaatta gatacagcca gtggtggcgg ttttctttac tgtaatgcct ctgacagggc
     5581 aaatccagat ggactaatgg atattgaaaa tgccatggtt gattctggta aaacctatgg
     5641 cgggcataag ttatttaaaa cctctgtccc tggcttgtat tacaccctat ttattgcaaa
     5701 actttggtct gcatattcaa caaaaacgac tattagcgac tctggtttat atgttggaga
     5761 taccacaccc caaaaattcc agtggatcat cacagaccct aatctacaac atgacggctg
     5821 cgataacgcg atatggtacg atagattctg ggccatcggc ggtgttgtac acgaccttac
     5881 gattgaattt tatactgata ccgattttaa ccctacaaca aaccagcaag tatccttatc
     5941 cagctcatcg acatatcttt atgcgtttaa ggcctatagt cccggtactc gtgttgttga
     6001 tcacagtcat cacctttata tcgactttga tctactcaat gttaaattaa ccaaccccac
     6061 ctgctttact gctgtactta ccggaaaatc agtaagtggt tcgacggtaa aaatgggaga
     6121 atatgcacca gggcagataa aaaatggcgc gactccggtt ccttttgata tttctctcga
     6181 aaactgtgtt cgcgttggtg atatagagac aaagctatct tcagggaagc tgggtacaga
     6241 aaataagcaa ctccttggta acacaatcac tggaagcgac gcagcaaaag gcgttggggt
     6301 attgattgaa ggattagcaa acagtaaaag tgccctaatg atactgaagc cgaatgattc
     6361 agcttctgtt tataaagata atacgggcca gacgcagaat aacgattcag atgcaattta
     6421 cccagaagat caaggtataa cgtatccact tcatttccag gcgacgttaa agcaagacgg
     6481 gaatattgct atcgaacccg gcgaatttaa agccaccagt actttccagg taacctatcc
     6541 ctgataacat tgtggcaccg tctggtaatg gcggtgtcac aacagagaaa tcattgcccc
     6601 caccaatgtg actgataaaa tgccagacag cacaacatca tttatcagga taccttatga
     6661 agccgaccac catctcctta ctgcagaagt acaaacagga aaaaaaacgt ttcgcaacca
     6721 tcaccgctta cgactacagc ttcgcgaaac tgtttgctga tgaaggactt aacgtcatgc
     6781 tggtaggcga ttcgctgggc atgacggttc agggccacga ctctaccctt cccgttaccg
     6841 tcgaagatat cgcctatcat accgccgccg tacgtcgcgg cgcgccaaac tgcctgctgc
     6901 tggctgacct gccgtttatg gcgtatgcca cgccggaaca agccttcgaa aacgccgcaa
     6961 cggttatgcg tgccggtgct aacatggtca aaattgaagg cggtgagtgg ctggtagaaa
     7021 ccgtacaaat gctgaccgaa cgcgcggttc ctgtatgtgg tcacttaggt ttaaccccac
     7081 agtcagtgaa tattttcggt ggctacaaag ttcaggggcg cggcgatgaa gcgggcgatc
     7141 aactgctcag cgatgcatta gccttagaag ctgctggggc acagctgctg gtgctggaat
     7201 gcgtgccggt tgaactggca aaacgtatta ccgaagcact ggcgatcccg gttattggca
     7261 ttggcgcagg caacgtcact gacgggcaga tcctggtgat gcacgacgcc tttggtatta
     7321 ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc cgaaacgggc gacatccgcg
     7381 cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt ttatccgggc gaagaacaca
     7441 gtttccatta aggagtcacg ttgtgttaat tatcgaaacc ctgccgctgc tgcgtcagca
     7501 aattcgccgc ctgcgtatgg aaggcaagcg tgtggcgctg gtgcctacca tgggtaacct
     7561 gcacgatggc catatgaagc tggtcgacga agccaaagcc cgcgccgatg tggtcgtcgt
     7621 cagtattttc gttaacccga tgcagttcga ccgcccggaa gatctggcac gttatccacg
     7681 gaccttgcag gaggactgcg aaaagctaaa caaacgtaaa gtagatttag ttttcgcccc
     7741 ttcggtaaaa gagatctacc cgaacggtac tgaaacccac acttacgttg acgttcctgg
     7801 tctttccacc atgctggaag gtgccagccg tcctggccat tttcgcggcg tttcgactat
     7861 tgtcagcaag ctgttcaacc tggtccagcc ggacatcgcc tgcttcggtg aaaaagattt
     7921 tcagcaactg gcgctgatcc gcaaaatggt tgccgatatg ggcttcgata ttgagatagt
     7981 cggtgtgcca attatgcgcg ccaaagacgg tctggcgctg agttcccgta acggttatct
     8041 gacggcggaa caacgtaaaa ttgcgcccgg tctgtacaaa gttttaagtt cgattgccga
     8101 caaattgcag gctggcgaac gggatctcga tgaaattatt gctattgccg ggcaagaact
     8161 gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc gatgccgaca cgctgctgga
     8221 agtctctgaa accagcaaac gagctgtgat tctggtagcc gcctggcttg gcgatgcccg
     8281 cctgatcgac aataaaatcg tcgaactggc gtaatgcttt tactctaaat aattcaagtt
     8341 gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg aatttgccca gccgcaggct
     8401 ggccttcggt gagagacatg gatgtctctc attaatcccc aggagcttac ataagtaagt
     8461 gactggggtg agcgaacgca ggcgcagcac atgcaacttg aagtatgacg agtataactg
     8521 gcgctacggc tgatggcgcc aattattaat tgccacgtta tctgtaagca attattcata
     8581 acgcagacaa cttagttaga gttaattagc ttatcaatct cagcaagaga taaatttgtc
     8641 atctctgcaa cgtcttcccg agacatccct ttactcagaa gacgctgggc gaattcctga
     8701 cttacttcct gtcttccctg ctgaatcccc ttctcaatcc ctttctcttc aaaccactgc
     8761 gccagcgtca tcatagacat ccctcccgtt tccctgtctc tcaccacacc ataaaacaaa
     8821 tccgcttgtt cagtatgacc gcgttgcagc atatagtttt gcatggcaac taactgactt
     8881 ccgctagtgt acccttcgtc gatcagcgtg accagttgct ccagcaataa cattaagtcg
     8941 cgctggcgaa tatgtttttg cagtagttcg agaatcgcaa tccgccgatg ttgcatgatt
     9001 tcgtcatccg gtgtgatggt gatatccacc tgcgggaaag gactgttata gacgcgtcgc
     9061 gccagctccg gcgagtaaaa catatcaaac cagcacattg atagcggata aggtgtggcc
     9121 tcgccctgat aaaacagtat cggcaccacc agcggcagct tatcgtgatc cgcctccaga
     9181 tgccggtgca tggcagcaat agaataacgc atcatgcgaa aggccatttt cttatccggc
     9241 ttgctttggt gttcaatcac aacatgcaga taaccgggat tgccctgcat ttgtacggaa
     9301 tagagcacgt ccgtgctgtg tcctttcagg ctctcttcaa tgaaactccc cgactctaaa
     9361 tgaagcgtgt tgaggtcaca aagttcgcgc aattccactg gcaaatgtat ctccagaaag
     9421 tcgcgagccg tctccgcatg cattaaaaat tgtttaaata ccgcgtcatg cggtgtggta
     9481 ctcggtgcat ccatcttccc tgtcttccgt gttgtcttgc gtgaaggcag tatccagtca
     9541 aggcgtgcta tcgtcagcca cctctttatt gtttaccgag ccgcgttcag agaaatatca
     9601 attcgttaca ggcgatacat agcacgcttc ggcgcggtac gcaaactgct gatgcaaatg
     9661 ccgtcagatg agtagacact aaacaaaaat cgggcaatac tgcgtgagaa tttcctcaaa
     9721 gcaggcatcg cctgcttcgt taacgacagg gtagaaaggt agaagttatg attcgcacga
     9781 tgctgcaggg caaactccac cgcgtgaaag tgactcatgc ggacctgcac tatgaaggtt
     9841 cttgcgccat tgaccaggat tttcttgacg cagccggtat tctcgaaaac gaagccattg
     9901 atatctggaa tgtcaccaac ggcaagcgtt tctccactta tgccatcgcg gcagaacgcg
     9961 gttcgagaat tatttctgtt aacggtgcgg cggcccactg cgccagcgtc ggcgatattg
    10021 tcatcatcgc caacttcgtt accatgcctg atgaagaagc tcgcacctgg cgacccaacg
    10081 tcgcctattt tgaaggcgac aatgaaatga aacgtaccgc gaaagcgatt ccggtacagg
    10141 ttgcttgatt gttatccctg cggctggtta ctcaccagcc gcgacatcgt ctcgagcgaa
    10201 tccgttctta aaatataaag tcgttttagt aacaacggat tatcccccgg ttttactttg
    10261 cctttcatag ttgtcaccgc caggtgaaat ccggcatcgt ttgctgcctt cacggctttg
    10321 tcattaaagc cgccaaacgg atacgaaaga taccagacat gtggattaaa ttgcgccaga
    10381 gcgcggcgtg aacgtgcaaa atcaaacaga atattgtgct cactacggct cagtaatatg
    10441 ggtcggcgat aaccatctac ccgatgcaaa aaatgggtat gtgactggaa atcaaaaaca
    10501 tcgcgaattt cgttaagctc agaaacgctc ataaattgca gcgattttgg gtcccatttc
    10561 tgcgggtgac gtttgatgcg tgaggtaaca ataaacgccg tcgccttcat gccatattgt
    10621 ttcaacacag gatacgcata gcggctcacc gacttgaggc catcatcaaa ggtaatcacc
    10681 accgctcgcg cagggagatt gatcttattc ttcacgtaac cttccagctg cgccatgctc
    10741 agtgtcgcgt atcccctgtc gcgcagccag gccatctggt tattgaaagc gcgtactgag
    10801 gtggtcgtcg aagtatgacg aaaacgggtg ttttcttcgt cgcgcagaat atggtgatag
    10861 gttagcaccg gcaggccatt atcgggttgg gcatccagcg cgctgatata ggccagtcga
    10921 tcgccaatac gaacctgata ccaggtttga tttaacctgt ctttcagttt atgcaaaatc
    10981 gggtagcgca aattgtccgc cagtacccca aatggcgcac tgcccgcact cggcgcgtta
    11041 tagaccggcg tatctttcca ggtaactaag ttctgattac tcagcggctt gttgaggtca
    11101 cccaaaccgt cttcaacttt ttgtcgcccc tgaaccggct cgagatgccc tttatcgata
    11161 aaccctttac caaagccaaa attaaatacg taataacttg cggcagtggg ctccacggca
    11221 atgatttgtc cggcccgaat attccccacg gtcaccatct tgtcaccaat ttgcgcccag
    11281 accgcagcat tttcgatggt ttgcatataa cgggcaggta acgcggcact gacacttgcg
    11341 gtaaacagca tcagcaggag aataacagct tgtttataca tgaaaaaata accgagggtg
    11401 aaacaacgct tttattgtag caaaagcgcc atcagaatcg cgggttaata ctcatacaaa
    11461 ttatgtttga ggacgaaagg cggatttttt tgttgttggt gccaaagact actcacctcc
    11521 ggcccaccca gttcgacaat acgctcacga aactctgaac tggtcatggt ttcacggcaa
    11581 gccatcatct gttcaattaa cggtaacgtg acaccagaaa tcacttcgca acgggagtgt
    11641 ttatggctta ataatgaagc cacgcggtac ggtggcgctc cggctatatc ggttaagaag
    11701 atgacaccct caccagagtc cgcttcatgc agagcatcgc acatcatgcg gctgagcata
    11761 ttagagctta atccgcgcca gaaattcacg gcccggcact gaagaagttc cccatgtttt
    11821 ttctccagcg catccagtat ctcttgcgcc cggtcatcgt gacaggtaat tacccaacct
    11881 aacatcgcct tcctccttag ccgaaaatca gtctaccctg accattgttg ataaagggtg
    11941 atagccgtca aaactcctct cccccaaatc cagaggagag gaaatagcct tagctacgca
    12001 aaccacgtcc acgttggatc agcgaccaac agatcaaata aaacgccaca ataaagacca
    12061 ccagtacgcc aaaggtagtg accagcggca catcattgat accgaggaag ccgtagcgga
    12121 aaccactgat catataaacg attgggttca ggtgcgacag cccttgccag aacggcggca
    12181 acaaagtcag ggagtaaaac accccgccca aatacgtgag tggcgttaac acaaaggttg
    12241 gcaccaggct gatgtcatcg aacgttttgg caaacacgcc gttcagcaaa cccgcaaggg
    12301 agaatagcac cgccgtgagc accagcgtta aggcaacgaa tacccacgaa tgcacctgaa
    12361 atggcacaaa aaacagtgaa attgccgtca ccagaatgcc aacaaacaga ccacgcgcca
    12421 cgccaccgcc gacatatccg gcaataatga cgtgagtcgg aaccggcgct accagcagct
    12481 cttcaatatt acgctggaac ttggcaccaa aaaatgatga cgcgacgttg gcgtaggcat
    12541 tggtgatcac cgacatcatg atcagccccg gtacgatgaa ctgcatgtag ctgaagccat
    12601 gcatatcgcc aatacgcgaa ccaatcaggt taccgaaaat aataaagtaa agggtcatgg
    12661 tgatgactgg cggcaccagc gtctgcaccc agatacgcat aaagcgatgg atctctttcg
    12721 cccagatgct ttttagcgcc acccagtaaa gatgcatcat gcgcgatctc cttgtttttc
    12781 attaaccagt gaaacaaaca gctcttccag acggttagct ttgttacgca tacttaatac
    12841 ctgaatgccc tgctcactta actgcgaaaa tacgctgttg atcccctgct cacgcagcac
    12901 ttcaacttcc agcgtcgcag tatcgaccag tcgatactga tagccatcga gcttcggtaa
    12961 cgggcttttc ggtgcgagat cgagaataaa ggtttccgat ttcagcttcg ccagcagcgc
    13021 cttcatcgag gtattttcca ccagctcacc gtgttgaata atgccgatat tgcggcacag
    13081 catttctgct tcttccaggt agtgtgtggt gagaatgatg gtggtgcctt tgtcgtttaa
    13141 atccttcaaa aagccccaca ttgagcggcg aagttcaata tccacgcctg cagtcggctc
    13201 gtcgagaatc agtagtttag gttcatgcat taacgcacgg gcaatcatta aacggcgctt
    13261 catcccgcca gataacatgc gcgcacgttc gttgcgtttt ccccataaat cgagctgctt
    13321 aagatatttt tcgctgcgga tgtacgcctc tttgcgctcc acaccgtagt accctgcctg
    13381 attcaccaca atttgctgca cggtttcaaa cgggttgaag ttaaattcct gcggcaccag
    13441 tcccaactga cgtttagcgt tcacgacatc tttctcgaga tcgtaaccaa atacgctgac
    13501 ccgcccggag gttttattta ccagagagct gataataccg atagtggtcg atttcccggc
    13561 cccgttcggc ccgagaagcg cataaaaatc acccgcttcg acctgcaaat ctattccacg
    13621 aagcgcctga acgccgcctg gataggtttt tttaagctgt tgaagttcca gtgcaatggt
    13681 cataaatttt tacttacctt acgttcttac actttatctg tggtttaaat cgtcccggag
    13741 ttgccctata ttagccaaac gtaattattt ggttacaggt cgttaacctc catgaaagac
    13801 atagatacac tcatcagcaa caatgcacta tggtcaaaaa tgctggtgga agaggatccc
    13861 gggttttttg agaaactggc acaagcgcaa aaaccgcgct ttctatggat tggatgttcc
    13921 gacagtcgcg ttcctgcaga acgtttaacc ggtcttgagc cgggcgaact ctttgttcac
    13981 cgtaatgttg ctaacctggt cattcacact gacctgaact gcctttccgt ggttcagtat
    14041 gcagtggatg tactcgaagt tgaacacatt attatctgtg gccactacgg ttgcggcggc
    14101 gtacaagccg cagttgaaaa cccggaactg gggcttatca acaactggct gctgcatatc
    14161 cgcgatatct ggttcaaaca tagctcattg ctcggcgaaa tgccgcaaga gcgccgtctg
    14221 gataccttgt gtgaactgaa cgtcatggaa caggtgtata acctgggcca ctccaccatt
    14281 atgcaatcag cgtggaaacg cgggcagaaa gttaccattc acggctgggc ctatggcatt
    14341 cacgacggct tgctgcgtga tctggatgtt actgccacca accgcgaaac ccttgagcaa
    14401 cgttaccgtc acgggatttc caacctcaag ctgaaacacg ccaaccacaa ataaaaatgc
    14461 catgccggat gcaacacatc cggcaacttc acacttactc gtccagcaga atcactttgc
    14521 cgatatacgg cagatgacga taacgctgag cgtaatcaat gccgtaaccc accacaaact
    14581 catccgggat cgagaaaccg ataaattcta ccgggacgtt cacttcacga cgggacggtt
    14641 tatccagcag cgtacaaatc gccagcgact tcggttcgcg caggcttaag atctcacgca
    14701 ctttcgacag tgtattcccc gagtcgatga tatcttcaac aatcagcacg tccttgccac
    14761 ggatatcttc atccagatct ttgaggattt tcacatcacg ggtggtggac atgccgctac
    14821 cgtagctgga ggcggtcata aagtcgactt catgagatac ctgaacttca cggcacaggt
    14881 ccgccataaa cataaatgag ccacgcagca gacccaccag caccatatcg ctgccgctgt
    14941 ctttgtaacg ctcagtaatc tgacgaccca gttcggcgat acgcgcttta atctccgctt
    15001 cggggatcat tacttctaca gtatgtttca tatctctaac catatgattt aaaaacaaat
    15061 cacttcgcac gggcaggttt tggcaccgca acaaagcgca agacacgcag tataccagta
    15121 aaactattca ttcgcggaat ctgttaataa agctcgaatt gtgatgacga tcacacatgt
    15181 taaaacctat aattaattgc tactaaaata ttaatgaatt gaaatggtgt ctctttatgg
    15241 caattaacaa tacaggctcg cgacgattac tcatcacgct aacagccctt tttgcagcgc
    15301 tttgcgggct gtatctactc attggcggag gctggctggt cgcgattggc ggctcctggt
    15361 actaccctat cgctggcctt gtgatgctcg gcgtcgcctg gatgctgtgg cgcagtaaac
    15421 gcgccgcgct ttggctgtac gcagccctgc tgctcggcac catgatttgg ggcgtcgggg
    15481 aagttggttt cgacttctgg gcgctgactc cgcgcagcga cattctggtc ttcttcggca
    15541 tctggctgat cctgccgttt gtctggcgtc gcctggtcat tcctgccagc ggcgcagttg
    15601 ccgcactggt ggtcgcactg ctgattagcg gtggtatcct gacctgggcc ggatttaacg
    15661 atccgcagga aatcaacggc accttaagcg ccgatgccac acctgccgaa gctatctcac
    15721 ccgttgccga tcaggactgg cctgcctatg gtcgcaatca ggaaggtcaa cgcttttcac
    15781 ctctgaaaca aattaacgcc gataacgtcc ataatctgaa agaagcctgg gtgttccgta
    15841 ctggcgatgt gaagcagcct aacgatccgg gtgaaatcac caatgaagtg acgccgatta
    15901 aagtgggcga caccctttac ctgtgtaccg ctcaccagcg cctgtttgca ctcgatgccg
    15961 ccagcggcaa agagaaatgg cattacgatc ctgagctgaa aaccaacgag tctttccagc
    16021 acgtaacctg ccgtggcgtc tcttatcatg aagccaaagc agaaacggct tcgccggaag
    16081 tgatggcgga ttgcccgcgt cgtatcattc ttccggtcaa tgatggtcgc ctgattgcga
    16141 ttaacgctga aaacggcaaa ctgtgcgaaa ccttcgccaa taaaggcgtg ctcaatctgc
    16201 aaagcaatat gccagacacc aaaccgggtc tgtatgagcc gacttcgccg ccgattatca
    16261 ccgataaaac catcgtgatg gccggttcag ttaccgataa cttctcaacc cgcgaaacgt
    16321 ctggcgtgat ccgtggtttt gatgtcaaca ccggggagct gctgtgggct tttgatcccg
    16381 gcgcgaaaga tccgaacgca atcccgtctg acgaacacac ctttaccttt aactcgccaa
    16441 actcgtgggc accagcggcc tatgacgcga agctggatct ggtctatctg ccgatgggcg
    16501 tgaccacgcc ggatatctgg ggcggtaacc gcacaccgga acaggaacgt tatgccagct
    16561 cgattctggc gctgaatgcc acaaccggga aattggcgtg gagctaccag accgttcacc
    16621 acgacctgtg ggatatggat cttccggcac agccgacgct ggcggacatc accgttaatg
    16681 gtcagaaagt gccagttatt tacgctccgg cgaaaaccgg caacattttt gtgctcgatc
    16741 gtcgtaatgg cgaactggtg gttccggcac cggaaaaacc ggttccccaa ggtgctgcca
    16801 aaggcgatta cgttacccct actcaaccat tctcggaact gagcttccgt ccgacgaaag
    16861 atttgagcgg tgcggatatg tggggagcca ccatgtttga ccaactggtg tgccgcgtga
    16921 tgttccacca gatgcgctat gaaggcattt tcaccccgcc atctgaacag ggtacgctgg
    16981 tcttcccggg taacctggga atgttcgaat ggggcgggat ttccgttgat ccaaatcgtg
    17041 aagtggcaat tgccaaccca atggcactgc cgtttgtttc gaaactgatc ccacgcggtc
    17101 cgggtaaccc gatggaacag ccgaaagatg ccaaaggcac gggtacggaa tccggcattc
    17161 agccacagta cggtgtaccg tatggtgtca cgctcaaccc gttcctctca ccatttggtc
    17221 tgccatgtaa acagccagca tggggttata tctcggcgct ggatctgaaa accaatgaag
    17281 tggtgtggaa gaaacgtatt ggtactccgc aggacagtat gccgttcccg atgccggttc
    17341 cggtgccgtt caatatgggt atgccgatgc tgggcgggcc aatctccacg gcgggtaacg
    17401 tgctgtttat cgccgctacg gcagataact acctgcgcgc ttacaacatg agcaacggtg
    17461 aaaaactgtg gcagggtcgt ttaccagcgg gtggtcaggc gacgccaatg acctatgaag
    17521 tgaatggtaa gcagtatgtg gtgatctccg caggcggtca cggttcattt ggtacgaaga
    17581 tgggcgacta tattgtggct tatgccctgc cggatgatgt gaaataacca taatgcccgg
    17641 agagatccgg gcatatttcc gaatacggtc tttttatacc gtaaacccta acatcatccc
    17701 cgtatcttca tgctccagca gatggcagtg cgccatataa gcatgttctt tcggtgcgtc
    17761 gtgattaaac ttcaccagca cttcactgac attgccttct accttaacgg tatctttcca
    17821 gcccgcgcga tgagccgctg gcggtttgcc attttctgac aagatacgga actgcgtgcc
    17881 gtgaatatgg aacggatgca gcatcatgtc gcccacgcca gagataaccc aacgttcgta
    17941 ctgccctttc gccgccgcaa acatcggctt attcatatca aacgcctgac cgttgatttt
    18001 attggcatgg tggaaatcga acttcccgcc gtggttcata tgattcatat tgccgtgccc
    18061 catatggccc atcatctggc tatgatccat cccggccatc gcctgatcgc catatttctc
    18121 cattagcatc tgcatcccca tcatatcgag catcgggtcc atagaaagtt gcagcttgcg
    18181 taccgtcagc ccttccagcg aaggtaacgc aggcaggcta cttaatgtgt ctggcaaagc
    18241 accggaggca ctaatagcaa tcggctgaat ccgcattacc ggatgaggct tatcaaacgg
    18301 cgcaatcgcc atccccatct ggctgaccgg cagcgtcacc aggtcaaagg gtttgttgtc
    18361 gttaacctcc accagcactt caaaacgctc gcccatcagc accggcagct cgttcacctt
    18421 caccggttca ggtagcagac caccgtcgct ggcaatcaca taaagcgggc gattgtcgct
    18481 ggtggcgaaa ttgagcgagc gggcattaca gccattgagc aaacgcaggc gcagccaacc
    18541 acgcggggca gcgtgttgcg ggtagattgc accgttggtc agcaacgtat cgccaaacca
    18601 gcccacggcg gcggtcatca catccagttg ataatcaatc tgcccgtcgg cgctaaattt
    18661 cttatcctga acgatcaccg gaacatcatc gataccccac tgttttggca gcattaattt
    18721 caggatctcg tcatcttcaa tcaccaccag accagccagc cccatcgcca cctgtcgccc
    18781 ggtcttgcca tgttgatgtg gatggaacca gcaggtagcg gcaggttgat caacgttcaa
    18841 cgtcaccgag cgcttgccac ctggcggaat aattccctgt gggccaccgt cgacttcacc
    18901 cggtacttcc agcccgtgcc agtgcaacgt cgtctcttcc gtcagttggt tatagatatc
    18961 aaccgttacc gctttgccgc gctgtaattt caccgccggc cccagcagat tgccgttata
    19021 gccccaggta gttgcagttt tctcaccaaa ggtagactga cctgcgccaa tagttaactg
    19081 aatgcgatta cgggcatcgg tcgtgagcaa atcagggatc ggtaacgttg ggcgttccgc
    19141 cgcaaagact gcacggctcc acagtggcaa ggctgaagcc acacccagcg cgacggaata
    19201 tttcaagaaa tcacgacgtt gcatagttat ttccttattc ttaagcaggc gaaacaaaat
    19261 caaacgttga gcatagtcct tccccttacg ggaaggtcaa gccgcagaca caatttaatc
    19321 gacaataatg gtcgtcgcct cgctcgcagt gtgctaacgt ttatcttctt taagcccctg
    19381 gtagaagcaa tgaagacgtt tttcagaaca gtgttattcg gcagcctgat agccgtttgc
    19441 gcaaacagtt acgcgctcag cgagtctgaa gccgaagata tggccgattt aacggcagtt
    19501 tttgtctttc tgaagaacga ttgtggttac cagaacttac ctaacgggca aattcgtcgc
    19561 gcactggtct ttttcgccca gcaaaaccag tgggatctca gtaattacga caccttcgac
    19621 atgaaagctc tcggtgaaga cagctaccgc gatctcagcg gcattggcat tcctgtcgct
    19681 aaaaaatgca aagccctggc ccgcgattcc ttaagcctgc ttgcctacgt caaataatcc
    19741 ctgatacctt tttgtagaaa tattgaccgt gcatccgcgg tcaatgttag ctattatgtt
    19801 gcgccctttt tttacgggtg ttaacaaagg aggtatcaac ccatggccga aaaaaaacag
    19861 tggcatgaaa cgctacacga ccagtttggg cagtactttg cggtagataa cgttctgtat
    19921 catgaaaaga ccgatcacca ggatctgatc atttttgaga acgctgcatt tggtcgcgta
    19981 atggcgctgg atggcgtagt acaaaccacc gagcgcgacg agtttatcta tcatgagatg
    20041 atgacccatg ttccgctact ggcccacggt cacgcgaaac atgtgctgat tatcggcggc
    20101 ggcgacggtg ccatgctgcg tgaagtaacc cgacataaaa acgttgagtc aatcacgatg
    20161 gtggaaatcg atgcgggtgt cgtatcgttc tgccgtcagt atctacccaa ccataacgcc
    20221 ggtagctacg acgatccgcg ctttaagctg gtgatcgatg atggcgtcaa tttcgttaat
    20281 caaaccagcc agacctttga tgtcattatc tccgactgca ccgatcctat cggtcccggc
    20341 gaaagccttt tcacttcggc attttatgaa ggctgcaaac gttgcctgaa tcctggcggt
    20401 atcttcgtcg cacaaaacgg cgtctgcttt ttacagcagg aagaagccat cgacagccat
    20461 cgcaaactca gccattactt cagcgacgtt ggcttttatc aggcggcgat cccgacctat
    20521 tacggcggta tcatgacttt tgcatgggcg acagataacg acgccttacg ccatctctca
    20581 accgaaatta ttcaggcgcg ttttctcgct tctggcctga aatgccgtta ttacaatcca
    20641 gcagtccata cggcagcttt tgccttacct cagtatctgc aagacgcact ggcttcacag
    20701 ccgtcctaag gagaagataa gaaattgaaa aaactgaaac tgcatggctt taataatctg
    20761 accaaaagtc tgagtttttg tatttacgat atctgctacg ccaaaactgc cgaagaacgc
    20821 gacggttata ttgcttatat cgatgaactc tataatgcca accgtctgac cgaaatcctg
    20881 tcagaaacct gttccattat cggggccaat attcttaaca tcgcccgcca ggattacgaa
    20941 ccacagggtg ccagcgtcac tattctggtg agtgaagaac cggttgaccc gaaactcatc
    21001 gacaaaacag aacaccccgg cccactgcca gaaacggtcg ttgcccatct cgataaaagt
    21061 catatttgcg tacataccta cccggaaagt catcctgaag gcggtttatg taccttccgc
    21121 gccgatattg aagtctctac ctgcggcgtg atttctccgc tgaaggcgct gaattacctg
    21181 atccaccagc ttgagtccga tatcgtaacc attgattatc gcgtgcgcgg ttttactcgc
    21241 gacattaacg gtatgaagca ctttatcgac catgagatta attcgattca gaactttatg
    21301 tctgacgata tgaaggcgct gtatgacatg gtggatgtaa acgtctatca ggaaaatatc
    21361 ttccatacca agatgttgct taaagagttc gaccttaagc actacatgtt ccacaccaaa
    21421 ccggaagatt taaccgacag cgagcgccag gaaattaccg ctgcgctgtg gaaagaaatg
    21481 cgcgagattt attacgggcg caatatgcca gctgtttaac ggctctggcg gagctccagg
    21541 ctccgccaga tttatttact tctgctgcac gaaattgcgg taagccgcca cgacctgcag
    21601 aaaatcctca acgccgcata gcgacaggct ttcttcgtcg tagtagttca tcccctcttc
    21661 catttcatcg ccagcgaatt ccagttgatt ggcgcgaacc atcacctctt caccgtccat
    21721 ccacagggtg tattcatgcc ctgcccgttg ccaggaccgt tcgctacctt tcagtgcgtg
    21781 cgctgcttgt tccacttcat caagcaaggc caggttttct ttcacctctt cattaaacca
    21841 gtgcccgacc acttcatgcc ccatggacat acgcaccttt accactccgg taatatcgcg
    21901 cagaaattcg taatccatag tgtgttcctc tgctcccggc aatgcgccga aatcgcattg
    21961 cgccattgat gtaattatcg cagccttacc ggagaaaaac agcggaacgg cgatggttta
    22021 gaaataaaaa atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg
    22081 tgcgcttact tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg
    22141 agaaagctgg ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc
    22201 aacgtaggtc tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc
    22261 cagttctgca gaagccagga agacattcgc gccagtcccc agacggttcg ggaagttacg
    22321 ggtagaggtg gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag
    22381 ggaacagcca gggatctcga tacgcgcacc gctcttaccg aagacgctat agtagccttc
    22441 ttcggtcaac tgcgcggcat ccatacgggt tggcggtgcc acccacaggc gcgtcggcaa
    22501 ctggcctttg tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat
    22561 gcaggaaccg ataaacactt cgtcgatctt ctcgccctgt accgcagaca gcggacgtgc
    22621 gtcgtccggg tcgttcggtg cacagaggat tggctcttta atatccgcca gatcgatgtc
    22681 gatcactgcc gcgtattccg catctgcatc ggcttccagc agctcaggat tcgccagcca
    22741 tttttccatg ccctgaatac gacgttccag ggtacgacga tcgccgtaac cttccgcgat
    22801 catccacttc agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag
    22861 cttgatggta caaccagcgg cagaacgctc ggcggacgca tcggtcagtt caaatgcctg
    22921 ctcaactttc agatccggca gaccttcaat ttccagaatg cgaccagaga agatgttttt
    22981 cttgcctttc ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg ggatcgcgtg
    23041 taccagatcg cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga
    23101 ttccggcata tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc
    23161 cgccgggaaa gagataccga tcgggaaacg ggtatgggag tcaccaccgg taccgacggt
    23221 atccggcagc agcatacggt tcagccagga gtggattacg ccgtcacccg gacgcagcga
    23281 cacaccacca cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg
    23341 cttcggatac gccgcggtat ggcagaaaga ctgcatcacc aggtcagccg agaagcccag
    23401 gcacgccagg tctttcagtt catcacgggt catcgggccg gtggtgtcct gtgaacctac
    23461 agaagtcatt ttcggttcgc agtacgcgcc cggacgaata cctttcacac cgcaggcacg
    23521 acctaccatt ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg
    23581 acggaacaca tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg
    23641 cccgataatc agcgggatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt
    23701 cagttcgaag gtcgccagca gttcgccggt ttcgtggtta cgcacttcac ctttgtacgg
    23761 gtaaacgtca atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc
    23821 acccgcgtct tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc
    23881 accgccgcgt ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt
    23941 cgtggcggat ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa
    24001 acctttctgt tgcagagctt caatctgttt gatcgggcca acaacacctg gctggtctgg
    24061 ctcaatacct tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg
    24121 cgaccacgca tccggtgccg gagaaaggtc atcagtgttg gtttcgccag tgactttgaa
    24181 gacggtaacg gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc
    24241 ccaggactgc attacctgct tcgcatattc gttgcctgct ttggctttct cttctacgtc
    24301 atagaagtta tcgaacatca gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag
    24361 tttggcatca tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtacc
    24421 cagcagttcg atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttagcgat
    24481 agcagccagg aagcctgctt tgacataggc ggcttcatcg acgccagggg gaacacggtt
    24541 ggttaataga tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac
    24601 aagtgcggcc atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc
    24661 agctacgtgc ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt
    24721 gcggcaggcg attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt
    24781 accgcaacgg gcagaatagc aggattttga cgatgtgtta atctgtttac aaaaaagcaa
    24841 cataaaaaag tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact
    24901 tcgttaattc gcatggtgat aatcaccttt ctcaacgaat accaagatta gtcaaaattt
    24961 aaactaccgc ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat
    25021 tgcgatttcc ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg
    25081 gcgctaattt gcagtgccgg gctttgtgcc gcctctaccg ggctatatat aaaaagccgc
    25141 acagtggaag cgcctgtgga accgcaatcg acacaacaga ctgcgcctga catcaccgca
    25201 gttacgcttc ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt caaatccgca
    25261 ttcagcactg cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc cgattctctg
    25321 ctttcacaag tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg
    25381 tcgcacgata atccacttaa acaaggcgat gctgctattc aggcggtatc tgaccagccg
    25441 tgggacgcca gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa
    25501 aacccgcaat gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg
    25561 gactcggtac agcgattgcg gcaactggcg caacaaaccg gttcgctgaa gtcatcaacc
    25621 gaacaaaaag ttattaccac aacgaagaaa gctgtaccgg taaaacagac agtcacggca
    25681 cccgtcatac catccaatac cgttttaact gccagccccg tcattacaga gccggcaaca
    25741 accgtcattt ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca
    25801 accgtggttt acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca
    25861 gccggagaac cgtttattga cagctttgtg cgcggattag gctatagcat gggtgttgct
    25921 accacgtacg cactattcag cagcatcgac tgggatgacg acgatcatga ccatcatcat
    25981 catgacgatg atgattatca tcaccacgat ggctgtcatc gtgacagtaa tggctggcaa
    26041 cacaacggcg acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat
    26101 cttactgata agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat
    26161 catgatcagg atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc
    26221 acgcagctac ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag
    26281 caacgaacac acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag
    26341 cggtttaatg aaactgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc
    26401 caacccctga cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc cgcctcgcct
    26461 gagcagcgcc aggcggttcg cgagaaaatg cagactaacc cacagaacca gcagcgaaga
    26521 gaggcagcgc gtgagcgtat tcagtctgca actcccgaac agcgccaggt gtttaaggaa
    26581 aaagtacagc agcgcccact gaaccaacag caacgtgata acgcccgcca gcgtatacaa
    26641 tcggcatcac ctgaacaacg tcaggttttt cgggaaaaag ttcaggagag ccgcccacaa
    26701 cgtctaaacg acagtaacca tactgtcagg ctgaataacg agcaacggtc agcagtacgc
    26761 gaacgtctct ctgagcgcgg agcaaggcga caggaaaggt aaattgcaga cgtaaaaaaa
    26821 gcggcgtggt tagccgcttt tttaattgcc ggatgttccg gcaaacgaac aattacttct
    26881 tcttcgcttt cgggttcggc aggtcggtaa tgctaccttc gaacacttct gccgccaggc
    26941 ccacagactc gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct tcagcatcac
    27001 aacccatttc gattgccagg ccgatttcac ccagcagctc gccgccgttg gtaccgacaa
    27061 tcgcaccacc gatcacacgg tgagattctt tgtcgaaaat cagcttggtc ataccgtctg
    27121 cgcagtcgga agcgatagca cgaccagaag cagcccacgg gaaggtggcg gtttcatagc
    27181 tgatgccttt ctctttcgct tctttctcag tcagacctac ccatgcaact tctggttcgg
    27241 tataggcgat ggacgggata actttcggat cgaagtagtg tttcttaccg gcgataactt
    27301 cagcggcaac gtgaccttcg tgaacacctt tgtgtgccag catcggctga ccgacgatat
    27361 cgccgatagc aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac
    27421 cacggtcgtc cacttccacg ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac
    27481 gaccaatcgc taccagcacg gcgtcgtaac gctgcggttc agcgggtgct tttttgcctt
    27541 ccatcgtcac ataaataccg tcttctttcg cttcaacggc ggtaactttg gtttccagca
    27601 tcaggttgaa tttcttgctg atacgcttgg tgaagacttt aacgatgtct ttgtcagctg
    27661 ccgggataac ctggtcgaac atttcaacca cgtcaatctg tgaacccagc gcgtggtata
    27721 cggtgcccat ttccagaccg atgataccgc cacccattac cagcaggcgt tctggtactt
    27781 ctttcagttc cagcgcgtca gtggagtccc aaatacgcgg atcttcatgc ggaataaacg
    27841 gcagttggat cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg atcacggttt
    27901 tgccgttctc accttcaact tccagggtgt tagccccggt gaatttaccc agaccgttga
    27961 ccactttgac tttgcggcct ttcgccatac cagccagacc accggtcagc tgattgatca
    28021 ctttctcttt ccaggtacga atcttgtcaa tatcggtttt cggttcgccg aagacgatac
    28081 cgtgttcagc cagcgctttg gcttcttcga taacttttgc tacgtgcagc agtgctttag
    28141 aagggataca gccgacgttc aggcaaacac cgccaagggt gttgtaacgt tctacgatta
    28201 cggtttccag acctaaatca gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc
    28261 caagtaccac gacctgagtt ttgatttcag tactcatcat gacctctata tatttatctc
    28321 cggcgggtct gacgtttttt ttatgacgcc cattatccac cgggtcgttc tatccgtcgg
    28381 tattttacaa aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc
    28441 taataacctc aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt
    28501 ttacttacat caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac
    28561 gagcaccatc agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca
    28621 gacgcggcac gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga
    28681 ggatagccac ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga
    28741 tgctggagat ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg
    28801 ctttcttaga aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt
    28861 ctttgaatac cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt
    28921 atttcttcag ggtcagacgc tgaccgtctt ccgacaacga actgttgaag cgaggcatct
    28981 gctcaagagc tgcagcaacg gctttcatga tgaagacaac cggggtgatc ttcacatcca
    29041 gcttacgttt cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat
    29101 cggttttgtc gaagtgagta acatgcggga tcatcaccca gttacggctc aggttcgcac
    29161 cagaaatttt ctggatacgg cccagttcca cttcttcgat ttcaccaaac ttgctgaagt
    29221 ccaccttcgg ccacggcagc atgcctggga taccgccgcc agtcgccgcc ggagctgctt
    29281 ctgcacgttt gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt
    29341 tacggccagt gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga
    29401 tcagcggagt cgcgtgaacg taagcgtcgt tttctgcaaa ttcagatttg ccttccgctt
    29461 tcgcagccgg tgctgctgcc ggagcttcag cttttgctgc cggagccggc gctgccgctt
    29521 cctgtttcgc cggagccgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg
    29581 agccagtttt cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg
    29641 gagccggaac ttccatagaa gctttgtcgc cttctacggt aatcagtgac tgttcagcgg
    29701 caactttgtc gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat
    29761 ccggaacgtt aacttctttc acgccagccg ctggtgcagg ggccgctgcc ggagccgctt
    29821 cctgtttagc cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca
    29881 gcgagccggt agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa
    29941 acggagccgg aacttccata gacgctttat cgccttctac ggtgatcagc gactgttcag
    30001 cttcaacttt atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga
    30061 tatccggaac gttaacatct tttgccgctg cagccgctgg tgctgctgcc ggagctgctt
    30121 ctttcttctc ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa
    30181 tcataatcag tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga
    30241 tacccgcctg cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg
    30301 actgttcggc ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat
    30361 cagccccgat gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag
    30421 acgcgggtta actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt
    30481 cttatcgatt tcgccacgtt tagccagttc gcccagcgcc gctacaacca cgtaggaagc
    30541 atcaacttcg aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt
    30601 acccagtacg cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt
    30661 catatagtcg gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac
    30721 gcgcggagtt tccagcgggt gcagcatgtt ccagcgttca caatcctgac cctctcgcgc
    30781 cagttcggtg aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag
    30841 gatctctgcc gcttcacgga cgtgacgcag gatggaaccg gagcccagca gctgaacttt
    30901 acctttgcta ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc
    30961 accttccggc attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac
    31021 gttctcttgt ttttcaccgt acatacgctc cagaccgtca tgcataatga cagcaacttc
    31081 gtaagcgtaa gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct
    31141 gtgaccatct tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc
    31201 gatcaggaag ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg
    31261 gaagccgaac atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta
    31321 agaggtcgcc gctgccagcc aggaacaacc tgcgcccaac tcgttgatcc cttcctgcag
    31381 aatctgacct ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcggggtgta
    31441 ctgctgaccg ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt
    31501 acgcgcttcg tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat
    31561 tacgttcagg gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag
    31621 cagcgcgccg aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg
    31681 gcttggcaga taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga
    31741 accttccggg aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt
    31801 gaaacggtcg cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat
    31861 gtttttacct tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac
    31921 tgtcgctttg cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc
    31981 gtgaccacca cggttcagtg cccagatctg ttcgtcagtc cagtctgcaa ccagtgctgc
    32041 ggtttccgga tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa
    32101 ggtctggtag tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc
    32161 tttacgcagc agttcatccc aacggctacc ccacatcact ttgatcacgt tccagccagc
    32221 accttcgaag atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc
    32281 aagacgctgc aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc
    32341 gatggtgatc gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta
    32401 aacggtctgt ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc
    32461 ctggtagata gcaccaatcg gacccagacc catagatacg gtcgggaact gccagaattc
    32521 cggcatcagt ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa
    32581 gttatccagc tgctcctgag tcagacgacc ttccaggaaa gcacgagcgt aaacgcccgg
    32641 ggagatgtgg ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa
    32701 gaagtggtta aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc
    32761 gcccagttcg aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat
    32821 agctgaacga atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac
    32881 ggggatggtg ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt
    32941 gcgggcttca gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg
    33001 gatgaccgat tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg
    33061 gaaacgttct gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct
    33121 gtcccattga actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc
    33181 ttttatatgc gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat
    33241 tttttcgttg ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt
    33301 cgagcaaaat ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg
    33361 gcttaccggc cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca
    33421 tctcgcgacg cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct
    33481 ccatacacct tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct
    33541 gatactggag tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta
    33601 tggcgtgatg gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt
    33661 aataagcggc gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgactcag
    33721 gatggtcgga gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga
    33781 caaaagtgcc gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg
    33841 cctcacgcaa ggagggacgg gagacgtcaa actgttttgc cagttcgcgt tccggtggga
    33901 gtttttcgcc cgggcggaga gtgccttcga ggatcaaaaa ctccagttgc tgctcaatca
    33961 catcggagag ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac
    34021 ttgccgaagt caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt
    34081 caccttatgt ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt
    34141 gacatatata acgtttcaaa gttgtaacta tgcacaaatg tagactttac gt
//
LOCUS       DAZKIV010000029        34155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZKIV010000029 DAZKIV010000000
VERSION     DAZKIV010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.029"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(117..359)
                     /gene="yiiF"
                     /locus_tag="ACHOT6_001487"
     CDS             complement(117..359)
                     /gene="yiiF"
                     /locus_tag="ACHOT6_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFP9946729.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(578..796)
                     /gene="yiiE"
                     /locus_tag="ACHOT6_001488"
     CDS             complement(578..796)
                     /gene="yiiE"
                     /locus_tag="ACHOT6_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFP9946730.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1401..1691)
                     /gene="nadS"
                     /locus_tag="ACHOT6_001489"
     CDS             complement(1401..1691)
                     /gene="nadS"
                     /locus_tag="ACHOT6_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFP9946731.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1692..2003)
                     /locus_tag="ACHOT6_001490"
     CDS             complement(1692..2003)
                     /locus_tag="ACHOT6_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9946732.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2232..3140
                     /locus_tag="ACHOT6_001491"
     CDS             2232..3140
                     /locus_tag="ACHOT6_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9946733.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3204..4145)
                     /gene="fabY"
                     /locus_tag="ACHOT6_001492"
                     /gene_synonym="yiiD"
     CDS             complement(3204..4145)
                     /gene="fabY"
                     /locus_tag="ACHOT6_001492"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFP9946734.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4190..4627)
                     /gene="dtd"
                     /locus_tag="ACHOT6_001493"
     CDS             complement(4190..4627)
                     /gene="dtd"
                     /locus_tag="ACHOT6_001493"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFP9946735.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4624..5496)
                     /gene="yihY"
                     /locus_tag="ACHOT6_001494"
     CDS             complement(4624..5496)
                     /gene="yihY"
                     /locus_tag="ACHOT6_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFP9946736.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5490..6089)
                     /gene="yihX"
                     /locus_tag="ACHOT6_001495"
     CDS             complement(5490..6089)
                     /gene="yihX"
                     /locus_tag="ACHOT6_001495"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFP9946737.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6230..7117)
                     /locus_tag="ACHOT6_001496"
     CDS             complement(6230..7117)
                     /locus_tag="ACHOT6_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFP9946738.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEF
                     VTPDKPLVIETRWQFTPES"
     gene            complement(7107..8456)
                     /locus_tag="ACHOT6_001497"
     CDS             complement(7107..8456)
                     /locus_tag="ACHOT6_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946739.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(8555..9988)
                     /locus_tag="ACHOT6_001498"
     CDS             complement(8555..9988)
                     /locus_tag="ACHOT6_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946740.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARIAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(10058..11068)
                     /locus_tag="ACHOT6_001499"
     CDS             complement(10058..11068)
                     /locus_tag="ACHOT6_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HFP9946741.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQSGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(11101..11988)
                     /locus_tag="ACHOT6_001500"
     CDS             complement(11101..11988)
                     /locus_tag="ACHOT6_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFP9946742.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFVAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(12013..12891)
                     /gene="yihU"
                     /locus_tag="ACHOT6_001501"
     CDS             complement(12013..12891)
                     /gene="yihU"
                     /locus_tag="ACHOT6_001501"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFP9946743.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            13064..13960
                     /locus_tag="ACHOT6_001502"
     CDS             13064..13960
                     /locus_tag="ACHOT6_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9946744.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            14000..14788
                     /locus_tag="ACHOT6_001503"
     CDS             14000..14788
                     /locus_tag="ACHOT6_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9946745.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(14894..16717)
                     /gene="typA"
                     /locus_tag="ACHOT6_001504"
     CDS             complement(14894..16717)
                     /gene="typA"
                     /locus_tag="ACHOT6_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFP9946746.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERMETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            17090..18499
                     /gene="glnA"
                     /locus_tag="ACHOT6_001505"
     CDS             17090..18499
                     /gene="glnA"
                     /locus_tag="ACHOT6_001505"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFP9946747.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            18673..19722
                     /gene="glnL"
                     /locus_tag="ACHOT6_001506"
     CDS             18673..19722
                     /gene="glnL"
                     /locus_tag="ACHOT6_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFP9946748.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            19734..21143
                     /gene="glnG"
                     /locus_tag="ACHOT6_001507"
     CDS             19734..21143
                     /gene="glnG"
                     /locus_tag="ACHOT6_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFP9946749.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            21255..21365
                     /gene="yshB"
                     /locus_tag="ACHOT6_001508"
     CDS             21255..21365
                     /gene="yshB"
                     /locus_tag="ACHOT6_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFP9946750.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(21555..22928)
                     /gene="hemN"
                     /locus_tag="ACHOT6_001509"
     CDS             complement(21555..22928)
                     /gene="hemN"
                     /locus_tag="ACHOT6_001509"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFP9946751.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(23117..23626)
                     /gene="yihI"
                     /locus_tag="ACHOT6_001510"
     CDS             complement(23117..23626)
                     /gene="yihI"
                     /locus_tag="ACHOT6_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFP9946752.1"
                     /translation="MKPSSSNSRSKGHAKVRRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(23745..23911)
                     /locus_tag="ACHOT6_001511"
                     /pseudo
     CDS             complement(23745..23911)
                     /locus_tag="ACHOT6_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24208..24840
                     /gene="yihA"
                     /locus_tag="ACHOT6_001512"
     CDS             24208..24840
                     /gene="yihA"
                     /locus_tag="ACHOT6_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFP9946753.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(25222..28008)
                     /gene="polA"
                     /locus_tag="ACHOT6_001513"
     CDS             complement(25222..28008)
                     /gene="polA"
                     /locus_tag="ACHOT6_001513"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFP9946754.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            28372..29304
                     /gene="yihG"
                     /locus_tag="ACHOT6_001514"
     CDS             28372..29304
                     /gene="yihG"
                     /locus_tag="ACHOT6_001514"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9946755.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(29345..30775)
                     /gene="yihF"
                     /locus_tag="ACHOT6_001515"
     CDS             complement(29345..30775)
                     /gene="yihF"
                     /locus_tag="ACHOT6_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFP9946756.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQDVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(30929..31555)
                     /gene="dsbA"
                     /locus_tag="ACHOT6_001516"
     CDS             complement(30929..31555)
                     /gene="dsbA"
                     /locus_tag="ACHOT6_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFP9946757.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(31572..32558)
                     /gene="srkA"
                     /locus_tag="ACHOT6_001517"
     CDS             complement(31572..32558)
                     /gene="srkA"
                     /locus_tag="ACHOT6_001517"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFP9946758.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(32635..32904)
                     /gene="yihD"
                     /locus_tag="ACHOT6_001518"
     CDS             complement(32635..32904)
                     /gene="yihD"
                     /locus_tag="ACHOT6_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFP9946759.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            32974..33558
                     /gene="mobA"
                     /locus_tag="ACHOT6_001519"
     CDS             32974..33558
                     /gene="mobA"
                     /locus_tag="ACHOT6_001519"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFP9946760.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     AQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            33540..34067
                     /gene="mobB"
                     /locus_tag="ACHOT6_001520"
     CDS             33540..34067
                     /gene="mobB"
                     /locus_tag="ACHOT6_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFP9946761.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         8584 a         8737 c         8186 g         8648 t
ORIGIN      
        1 atttctgcaa tatattgaat ttggaagaat ttgtaggccg gataaggcgt tcacgccgca
       61 tccggcatga acaacgcgca cgttgtcagc aatctgattg acatcatttt ttcccattac
      121 cattcctcgc gcagcttacg ctgatattcg acaccatctt cctcacaccc ttgccagatg
      181 ccaggaacac tggttcttgc tgtttgcgat tgatttatca ggtattgatc taccgcttcc
      241 cttaataggt ccgcacgagg aagattacgc tgcacctcga gatcatcaag ttgcttaatc
      301 acctcattcg ataaatcgag taaaattctg cccatatcta tacctgccag agagttcata
      361 aatatctcgc caatatcatt tttgaatcta tggagagaaa agtacccttg ccgaatcttt
      421 aaagaaagct catttacgca tcatttttta tttttgcgag gcgttttcca gatacgatgt
      481 aacagttgca gttattataa gttcaagtaa aacagccttg tattaatacc agaataaggg
      541 catctgtttt tttatattca agaatgaaaa atttttttca ttccttatgc tccttacact
      601 gatcatcaac accaactacc tccccctttt gcctgcctcc ctttgcttta cgcaccggaa
      661 attgctgccg ctccagatat tgctcaaccg cttcgcgtaa aattgcagat cgtgaaacct
      721 tacgccagcc ccgaagtttg ttcagccgtt taattgcctc ttctgataaa tgaagaaaaa
      781 ccgtattcat cgccattacc accaccttac ctatattact cattaatgta tgtgcgaaaa
      841 ttacgccgga aaatcattta aaaaaagcgg atttttgcat gttgttttgt ttttgcgagg
      901 cgttttccag aaataataag agtgttgcag ttgtttcatt tattactcaa aaatggttta
      961 gttatcgccc aggatgagtt atggatgcta tgaatatatt atattcaggg atgtttattt
     1021 tttgattgga tctttatttg cataatctga atggtgtacc cgaaaaatgt cagcatccta
     1081 acagcacccg gatttaaggt gaaattaatc atccatcaca gaccggtcag tactgccacc
     1141 aaccggtctt tacttgttca tcgttataat tttgttggta acagttcggg aaacaaggaa
     1201 ttccttccgg aaccccccgg tatcatccga tagcattgac gtcaatcatc agcgcgacga
     1261 tgagtttttt taatcgcatc atagggtggc gacgttcagc acgattacgg gtcatagcaa
     1321 aactcctggg caggcgacca ctatgccgct ttcacaggag ttctgaatgc tagtgctttc
     1381 tgtatggcat aaccatgcct ctaacggtta agtgcttgca gcacatgttg cggatcattg
     1441 gcaatcgctc gcagcaaagc ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag
     1501 ttacgcaacg tgtctacgct tacagaaata agatcggcaa atttagattg tgacaaccct
     1561 gaagcgcggc gtatttcttt gattttcata gcatcaactt caaaagtccg cgaaggctgt
     1621 cgcgttcctt caataatttc attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt
     1681 gccttatcca tttaccacct cgtatttaac aaccggagca ttgctttttc ctggggagac
     1741 agatcatctt taataccctt acgataaata agcagtaatc tgatctcata atgtttaacc
     1801 tgatgaaagt aaataaccct gacaccagca cgtttcccct ttcctcctga cacccatcga
     1861 acctttctta acccaccagt ttcaggtatt acctcaccat agtcaggatt caaggccagg
     1921 aaaaactgaa aacgactgaa ttcatcgtcg gtcagcagtt tttgaacatc ttcagtaaaa
     1981 atttccgttt cgatgaagag cataattata cgccattggc ttagagtaac aatccttttt
     2041 gtacgtcatt gacgtaccct tgaaaccact agcttactga tagtgctcca gcaaaaccac
     2101 gtctctgccg ggtttgtctt tagatttgcg aggcgctttc cagatttttc acgcccgtga
     2161 atttccctgt atccatccag caaaaacggg tgtataagac tgtacattca tacagcaaaa
     2221 taagttctac tatggcactg gaaaaaggaa ttgcagagtt agtcgaaaag tttatcgctg
     2281 cgggtcgccc ctcatcgcgt caacaaagta ttgcgcagcg cagggagggc tatatcgcca
     2341 gcgctgtcct tgcgggtgag acagaaacgc gggtagatat tcagactatc gaacttgaag
     2401 ggatgacctt acgaatagtg tcgccgctga atgccccgac attactcccg acgataattt
     2461 attactacgg tggctgtttt gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc
     2521 agttagccta ttacgggcaa tgtcgggtta ttgccgtcca gtatcggctg gcaccggaac
     2581 atactttccc cgccgcccat gatgatgcgc aacgaggtgc agaaatagtc tggcaacacg
     2641 ccgaacgact cggcgttgat aaacaacgta taactcttgc cggtgacagt gcgggcggac
     2701 atctggcgct ggtaactgct ttgcgattaa agcgtgctgg cgagtggcaa ccggcacaac
     2761 tcatcttaat ttatcctatg cttgatgcga ctgcgcattt cgaaagctac atacgcaatg
     2821 gtcacgatta catcatcaca cgcgacactt tactgagcgg ctttgagatg tatctgccag
     2881 gcattgaacg caggcatcct gaagccagcc ctctttggcg cgatgatttc aatggcttgc
     2941 cacctgtaca tatcattacc gctgaatacg acccactttg cgatgaaggt gaagcgttgt
     3001 atcaccgcat gacagggcaa ggagtgcaat gcacctgcca acgctacctt ggcgttatcc
     3061 acggattttt tcagttaggt ggtatcagcg aggccgcgcg atcggcacta cgggatgttg
     3121 cctggagagc gggaaggtaa cttcggacct gggccctgaa aaggttttca gattgaaaac
     3181 cttttcagtc aagtaaaacg cccctactct tcttcgttcc cgccctcttc atacgggcca
     3241 aatggcttcg cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg
     3301 ccaaagattt caacttgcat ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga
     3361 tcgccgctta aggcaccaag gtcggctacc gcatgaggtt taccgctaat cggcttgctg
     3421 tagcggatat gcgcatccgc cagaataatc gttccgccga ggtggcgttc acgcagcatc
     3481 agccagataa gtccccaacc ggtgagcgtc gccagcgaga ataaactccc ggcaaacaac
     3541 gtatggtgcg gattctgatt accggtttct ggcatggtgg tgataaattt ttgcccggta
     3601 tattgttgaa tacgcacgcc cattttttca ctaagcggga tatgttcgta ccacgcctgt
     3661 tgcagctgcg cgcaccagtc gccgcgatgc aaaatatcat ccagagtggc aacgggttta
     3721 atcatcaaaa aatggcgaat cggcgtggta gttggggtgg tgatctcccc ctgattaatg
     3781 aatcccagct tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt cacgcgctta
     3841 acgccttcct gacgcgccac cgactccagg gtcatcgcca tcagcgtgcc taaccctttg
     3901 tcctgcacgt ccgggtgaac ggccataaag cgaatggacg cttcattgtc ggcattaata
     3961 tacagtcggc ctaccgccac cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc
     4021 atcgcatccc acgcgtcgcg ttccgaacct tttggttgat gcaggggctt acgcaacatt
     4081 tcccagcgaa actgatagta acgctctaac tcttcttctg tttgtggaac ccgaaggtga
     4141 tacatagcga tactctctct tgtttcccgt gacaaccctg gaagctggct catacctgca
     4201 accagaatgt tacggggcca tcattgacca gcgatacctg catatcggca gcgaagcgtc
     4261 ctgtttgcgt gttcatctct tgctgacggc agcgttcgac gaaatagtca tataacgcct
     4321 ctgcgcgatc cggtgatgcg cctttggaga aacttgggcg catcccccgt tcggtatctg
     4381 cggcgagggt aaactgggaa accaccagca cactgccgcc cgcctgttgc acgttgaggt
     4441 tcatcttgcc ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg cacagacggt
     4501 ttgctttctg ttcgtcgtca tccttttcga cacccaataa caccaaaagt cccgcgccaa
     4561 tttcgcccgt cacttctccc tccacggtga cgctggcacg ggttacgcgt tgaattaatg
     4621 caatcatggt tcgtcgtctt cttcttgttc agctgcttgt tttagtttgc ggtattcccc
     4681 gagagtgaca gtaatttccg cgccaagcaa gacgatacac catgtccagt agacccaaac
     4741 aaagagaatg gggatcaccg ccaacacgcc gtaaatgagc tgatatgacg ggaacatggt
     4801 gatataaagc gcgaaacctt tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc
     4861 gacaatcgcg tcgcggttag gtacgcggat ggtaggaaca atgctgtaca gcaaccagaa
     4921 ggagatccac gacaacagca gcggaaaaat acgcagcacg ttatcgatga cagtattgag
     4981 atcgctcgcc cagcgcaggg agagcaaata ggaactgatc gccagactgg cccctgccag
     5041 cagcggcccc agcgttaaaa tcatccagta cacggcgaac gagtaaattt ttggtcgcgc
     5101 tcgtttactg cgccagatgg tattcaacgc gctatcgatg gagtacatca acaataacgc
     5161 ggtgacgatc agcccgcacg ccccaacggc ggtcatcttg ttggaattgg caacaaattg
     5221 ttcgatatac cgctgaataa catcgccagt agcaggcaga aagttggcaa aaataaagtg
     5281 acgtaactga atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac
     5341 ggcaaccagc ggcactaatg agagcaaaga cacataggca aggttacctg ccagggttgt
     5401 catgttgtcc tcatcaatac gttgccagag tagttttagc caggcccata gtggacgggt
     5461 acggtgcctg gctttgtcct gaatggtttt tagcataaca ccttcgcgaa atagtccggg
     5521 atggtggttt tatctttcac cagaatactg gtaatgccca gctgattggc tccttctata
     5581 ttatcggcgt tatcgtcgaa aaagaccgta tcgctgggtg aaaaaccttc cgcctgcaaa
     5641 acatgctggt aaattcgtgc ttcaggtttg cgcatcccca gatcttgcga cagatagata
     5701 tggtcagcag catcacgaat ttccgggtat tcttccggcc agaaggtggt atgcaggcgg
     5761 ttggtattgg aaagcaccac cacgcgatgc ccctgctcac gcagtttatg catgatggcg
     5821 atcacttccg ggcgcagcgc aacaaacacc gcctgccagc cgtgagagaa ctgctcgtag
     5881 cttagcggta gagccatctc atgacacagc gcctctgcga acgcttcgtc gctaatttcc
     5941 ccacgctcat gctgatgaaa cgcctccccc atatgaaaac tcttcttaag cgatgccagc
     6001 ggaatacgcg ttaaatcgct ccaggctccc agcacacggt taaagtcgat atcgacaatc
     6061 acattaccta aatcaaagat atagagcatt tttgcctcct ttcgcgccac aagaaattaa
     6121 ctgtagcggg aaaggatggg tttggctatg tggcaataag ggtaatcttg tgatgctgat
     6181 tgcgaaaaag cagagtaatt tcactggctt tttcgcaata gatgaatggt taagattcag
     6241 gtgtaaattg ccaacgagtt tcgatgacca gcggcttatc tggtgtcaca aattccggcg
     6301 ttagatttat tgcgttaggt gcctgagtca gcgggttaat acaggtggca tcgggctgtt
     6361 tatcaaatac caccagactg tttgcagaag agatcatggt catcgcgagt ttaccaggcc
     6421 acagtaactt aaccttcacc ccgttataaa aaccaaaaca gtcatcccag ggaccggctt
     6481 gaggagagat acgatttcca gttggcagtt catcactacc cgcttcttct tgccagtccg
     6541 catcaaaaag cacctgcaac gattctgtat tttgcggagt cagctttttc gcaaaccacg
     6601 gatgccagcc tgcggaggcc gggaatgtat cggcatatga atggacttcc agttgcaaaa
     6661 ccagcgcatc gttctccagc aaaaaagtct gaatcacttc cccttgccag ggccacggtg
     6721 aagccagggg catacgtaaa gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg
     6781 agtaacaagc cataccatgc aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt
     6841 gcccaccagc attcagcgtc gcattcccca gtcgccctgc ccacggcacc attgggaaac
     6901 agccatactg aaaagctctg cgctgcggct gccattggcg taatacttca gaaccaaatg
     6961 cctttaacga ggttatccta gcaccatcat gcggataaat ctgcaggtga atattgcctt
     7021 tttccagcgt cactaactct cctacaggcc caggccatgg cgcgtcaaac gcctgttgta
     7081 gcgccaggta atgctcgttt tttcgttcag ttttcatgag attgttccct gatgtagcgg
     7141 taagaagcgg tttcttgttc atcggtcggc tcaatcagcc agacgcaaac aaaggaaaga
     7201 actgagacag caaataaaaa cagagcgaca taccacggtt ttcctccccc tagcgccagc
     7261 aaacttgagg cgataagggg gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa
     7321 gaaaggccgg tatatcgtac tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg
     7381 gcaaacatca tcgtcggacc taagttgtag ccgaggatca tactgcacca gatgatgagc
     7441 gtacttttgc tatccagtaa taagaagaaa ggaaacgcga acaggacaca aaatcctgct
     7501 cccgacaaat acagactttt ttgacctata cgatctgata accatccaac caatggacaa
     7561 ctgaaaatac cgatcgccga ggcaatcaac atacccgtca ggggaatatc tcgcgacaat
     7621 gctaattggc tggaaatata gacaatacca aaggcgttaa taatattaga ggagacactt
     7681 tccgccagcc tcgcacccag tgccaataaa atatttcgtg gatgtttttt gaataattca
     7741 atcaacggag gaacggactt ttctttatta ttcgtcgttt tttgcttttg aaaatccagt
     7801 gtttcttcgg tatgcaggcg gataaacagc ccaacgatca acatcaccgc agaaagccaa
     7861 aacggaatgc gccatcccca ggagagaaac tgctccgaag taagaaaatg gttacagaga
     7921 gcaaaaatac cggttgccag cataataccc acagacgcag ccgtttgcgg gagtgaacct
     7981 aaaaatccgc gtcgggattc gggcgctgat tctatggtca tcaatgccgc tccgccgtac
     8041 tcgccaccaa gaccaaagcc ctgaatcaaa cgcagcacca ttaatacaag cggtgcccaa
     8101 atgccaattt cctgatacgt cggaataaaa ccaatcaaaa atgtggagca accgacaata
     8161 gccagcgtcc agataagtgt tatttttcgc ccgattttat cgccaaaatg accaaagacg
     8221 atgccaccta aaggacgagt cagaaaaccg acaccaaagg tcgcaaaggc ggctataaga
     8281 cctataaaag gatcgctgtc tggataaaac agcggtgcaa ataccagtcc tgctgccgtg
     8341 ccaaaaataa agaagtcata ccattccatt aacgcgcctg cgaaactgcc cataacgaca
     8401 cgtctcatgg ctttaccgga aagcccttca cgcggtgcgg gtaacttttc agacataatt
     8461 ttcctctcct agtaggcgag aaatctggga tacgaaccac taaacgtggg cagtttgaat
     8521 taatgcgcat cggtttaagc cgatgcgcat taatttattt cttcaattca gccagaacct
     8581 ctgcatcctc agcatcaaca tttgcaccca gtggttccca attaatcaat acgcaggtca
     8641 ggaaccccag gaatggcacg attgccaacc agtaaaacgc ttcagttttc aacgcacccc
     8701 acagcatcgg ccagaaaatg agtcctgcaa ttgcccctgt gcgcataata gcgcgggcaa
     8761 atccgacccc agtagggcga atagcgggag gataagagag tgtggcaatg gtcatgccca
     8821 gcccaccagg acccgctgaa tggaagaaaa taattgatcc caataaccct aaagacagcc
     8881 agggattagt cgttgccagt gcgcctaacg acagcaatgc taaggtcaca acagcaaatc
     8941 catacatcga ctgtcggcga gtacccagtc gttggagaat aaacgagccg atcatcccgc
     9001 ctgtcacccc acaaagggca ttcacgacgg cagaaccggt caacgcgcca gtaagcccgc
     9061 cacttattat tcccgccaac gtaagaggaa gataaacacc tacggcgtta tattgccacg
     9121 cctgcatggt tgccacgaca cagccaagaa tggtgcgttt gcggtaacga tcattaaata
     9181 agatgccata tccaccactg tatttattct ctgctttttc cgagaatttc gcttctgttg
     9241 tacccgatgc cgcaatgcga gcctgaacac cataacgttt tcccagaatg tcgcacgctt
     9301 cctggtaacg cccgacgcgt gcggcccaca ttgcggattc accaataaag aagtaacgca
     9361 aaatcatgaa gatgactgca aaaatagctc cgatgaatat tccgtaacgc cacaattgtt
     9421 cttcagcgat acccgacaga agcagcggca aaagtaccaa ataaacaacg gttgtcgaaa
     9481 cataccaggc catttgccag aggttgaccg acgttccggt tttcttcgac gttgaaccac
     9541 gaagctctgc cacggcattg gttgccaaag ggaagtcgat gcctaaacca aagcccatga
     9601 tgacgcggca caccagcacc caccagatac tgggagcaat agctaccgct gcagcaccga
     9661 tggtgcacag ccccatgcca ataataaatg cccgcttttg cccaaagcgg tttattactc
     9721 ggttcgctaa taaaccgcca attaacgcgg cgattaatac tgatgcattg acggttgcag
     9781 caagacccgg cgaaatgccg aattgagcgg caatgtattt attaccaaac cccaccatcg
     9841 ccgcctggta ggcatcaatc aatatgccgc ccaaagcaat aaaaataacg ccgatactgg
     9901 tgttgattgc agagttctta ttaacaatat caattacgtc ctgtggccga ctaattgaat
     9961 aagttgcagg actggtgatt tgactcatcg cagtgtcctt tttaattgtt gtagggtggc
    10021 gctgacgtga cgcagcgcca gacagagatt tagaaaatca ttccggtgca aattgcagca
    10081 tgacctttat ggagtcattt tgctgcgcgc gttcaaatgc cgccgctgca tcctcaatgg
    10141 caaataaatc ggtaaccagt tcactggtat caaaccgccc atccgccagc cattcgataa
    10201 cagcatcgaa atcttcaggg atgtacatgc cagagttgag caagtcacgc tcgaaacgtt
    10261 gcatcatcgg taatggtatt tcacgcggcc cacttggtac gcccatgcac acgacggtgc
    10321 ctccggcata tacacgggta catgcatcgt taagcgtggc ctgcgctgca acgacatcaa
    10381 tcgcccccgt aaatcgcaca tctgcggcca gctcacctgg tgcccaaact tctgctgcgc
    10441 ccagctttaa cgccagcgcg cgtttggcag cgtcaggttc aatcacggta atcttacctg
    10501 cccccataat gcgcagtgcc tgcacgatgg ataagcctat cgttccggca ccaattacca
    10561 gtacgctttc cagagaagct ttcggcattc ggttaacgca gtgacgggca caagccgccg
    10621 gttcggcaaa ggccaacact tttagcggta aagaggctgg tgcgacatgg caattacgcg
    10681 caggaacaat gtgttgtgag cgagcaaagc ccggtgcgcg aaaaccagca acctgcggtg
    10741 gttcacaaag attaaaacgt cctgctttgc aggctcggca ttccatgcaa gccatgatgg
    10801 gatcgaccac aacatgatcg cccgactgta catttttaac gtcgctgcca acttccgtaa
    10861 cgcgcgctgc aatttcatga ccggggacga caggtggttt ggcaaacgga tgaccgcctt
    10921 tcagaacatg cagatccgaa ccgcatatcc catagaacac aggcgcaatc cgcacttcgt
    10981 gcggtttaag ttcctggtgt ggtacttcaa catattgagt ggtgactttc ccaatatctg
    11041 aaaataaaac ctgggtaatt ttatccattg ttattctcca gaatcgatat ccatagggga
    11101 ttagcgttgc gcgatacctt catcgacaat ttctgccaac cgttgtaaac gaggaacaga
    11161 gacatcgcgc agcatagttt gtggatcttg tgcaccaaca accgacgccc aaaccgctcg
    11221 tccagccagg aagcccgacg caccaccttt catggcaata cttactgcac ggccaaaaat
    11281 atcggcatca acgccagaag aaagaattac ccacggcatc ttcatctgat cgttaagttg
    11341 ctgacaggcg gcaagtaagg ttttttcatc gcctttgccc cctaatggca tctcggcttt
    11401 gtaaagatca gcctctgttc caccaagctc tgcggcggcg gcaacaatgg cgctttcacg
    11461 atcaaagtcc caaccgcgac gaggtggacg caccacaggt tcaataatgc tcaccagccc
    11521 ggcactacgg cagcgacgaa caaatttatc aaccattgcc agacgttcct ctgcgggttc
    11581 atcttcacgc cagagtacaa gtaatttcat tgccttagcc cccatttcac gggctttatg
    11641 cgggtcgacg gacatatcta tttcaacgct atcaaccggg atgccattac ctgggataaa
    11701 aagatcggca gcgacaatca ggccgcaaga attagcaaca gcgccctgct cgactatttg
    11761 ctcaagacaa aattgtttat ccgccagaac ggcagaagca taaggagaaa ggattcgggt
    11821 cgcggcaact ttaaaatcgg tgagtacact gtcggcgatg ggttttggct gccctgctgc
    11881 cacaaacatc aagcgcatgg cctcacgctg atcgacagct aacatggcaa atccaccacc
    11941 tgggcgggag atatctttca gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt
    12001 taatagataa aattaaagac cagcctgctg gcgaacctga tttagcaggg cactccagtc
    12061 ctgccgccct ttgccgtctt gacttgctgc agcgtaataa ctttctgctg caatccccat
    12121 attgagcggt gcccccgtct cccgagcgac gtcaactgca atacgcagat ctttcaacgc
    12181 cagatccacc atgaaggcgg gtgaaagatc gcctttcagc actttgccag gccaggtggt
    12241 cgtaaaatgt cctttccctg ctgccgtacc actcatcacc ttaattgcga catcaagatt
    12301 taatcccagt gactcacata acacagcggc ctcggaggac agcgcattaa gcgcaatgct
    12361 catatagttg ttaattagct tcacgcggat ccccattcct gggccgccag cctcgaccag
    12421 ctcattaccc atgcacatga gaatcgggcg tgcacgtgca atctgctcat cagtgccacc
    12481 cgctaaaatc agcaatgtgc catcaatcgc attcacggac gttctgccca cgggagcgtc
    12541 catcatactg atgccttttt cttgtaactc ccgtattaat gcatcggtct gtaagggatg
    12601 aatagtcgac atatcaatca ataacgcgtc agatgaaaca gcctcacaga cgcctttttc
    12661 ccccaacaga acctgtcgaa caatgtcacc attgggaagc atagtgatga taaattcagc
    12721 gcctgctgct gcttccgctg gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc
    12781 gacggcctgt gcattgacat caaatacctg caatgaatgc cctttttgca atagattgtt
    12841 agccattggg ctacccatct gccccagccc gagaaatgca atggttgcca tgaatactct
    12901 cctgtctgac attgacaaaa taatgacaat tgaaaattcg ttttcgtctg tttttgagca
    12961 tatttgtaat ttatgttcaa aaacacgaca cgcatcacta atttgatcat ttgcgtcatt
    13021 taaattgacc aaagacgaca aaattaaaca ggacaagaca aatatgacca gaattgcatg
    13081 cgtgggtatc acagttcagg acagaattta ttctttgccc actctgccgg agggcggcgg
    13141 gaaatatcag gcaaatcatt atcttgagat tggtggcggc ccggcagcaa cagccgctgt
    13201 tgcaatcgca aagcttggcg tggaggttga tttcatcggt cgcgtcggcg atgacagttg
    13261 tggaaatacc ttacttgcag aacttgaggg ttggggcgtc aataccgcat tttgccgccg
    13321 atatcctaac gcccgttcgt cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat
    13381 tatcgttaat tatccaagtc ctgatctcgg tactgatgcc gaatggttag aagcgatcga
    13441 tttctctcgt tatgatctga ttctggccga tgttcgctgg catagcggta ctgaaaaagc
    13501 attctcgcta gcgcgccttg cgggtgtgac aacgctttta gacgccgata tgacaccgca
    13561 ggatatttct ccacttgtcg cgcttgccga tcacgccgtg ttctcaacac cagggctgaa
    13621 acgcatgact ggcctgcagt cacctgaaga aggtttgttc caggcaacga cacaaacagc
    13681 aggtaaagtg tatgttacgc tgggttctga aggatcgttg tggattgaag atggtcattt
    13741 gtgtcaacag gaggcatttt ctgtcaatgt ggttgatacc actggagcag gcgatgtttt
    13801 tcacggcgca ctcgcggtag cgttggcaga aaaaatgcca acaaaagagg ctattcgttt
    13861 tgccagtgcg gttgccgcga tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa
    13921 tcgtgagcaa accgaatcat ttttgtcact ttatgcgtaa aatggtaaac atctaccgag
    13981 gaggggcaag ggagtattca tgagtatcat cgaagtgaca ggtaatccac gccatgacca
    14041 actcgttcat ctaatcgcag aacgcggtta tatgaatatt gaagaactgg cacaactgct
    14101 ggatgtttca acgcaaaccg tgcgccggga tatccgaaaa ctcagtgagc agggcttgat
    14161 cacccgtcat catggagggg ccgggcgcgt ctccagcgtc atgaataccg cttttgagca
    14221 acgggaactt tcgctcaccg ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct
    14281 tcccgaacgc tgtaccgtct ttatcaccat cggtacaacc gtagaagccg ttgccagggc
    14341 attactcaac cggcgtgatt tacgcattat cactaacagc ctacgtgtgg cacagattct
    14401 ttataagaat caggatattg aagtgatggt gccgggaggc actttacgcg ctcataacgg
    14461 cgggattatc ggcccaggag ccgtggattt tattgaaggc ttccgcgcag attatttaat
    14521 caccagtata ggtgccattg aacatgacgg caccctactg gaatttgatg taaatgaagc
    14581 gttagtcgcg agaacgatga ttaaacatgc gcggaataca ttgttagtgg ccgatcatac
    14641 gaagtttgcc gcgtctgctg ccgtttcaat tggcaatgca cggaatgtca gggctttttt
    14701 tactgatgcc ccgcctccca attctttctg ccagttgtta agtgaagaga atgttgaact
    14761 ggtggttgcc gagcaagaag tatcctgaaa ttgaaatttc ggaaaatccg ctgggaattt
    14821 cagcggattt tgttttccat aagcaaaaaa aacctgccat cgctggcagg ttttttatga
    14881 ctaaaaaacg aaattaatcg tctttcggtg cgcggttggc gcgacgacga tcgttttccg
    14941 tcaggtgacg tttacgaata cggatagagg tcggggtcac ttctaccagt tcgtcatcat
    15001 cgatgaactc cagagcttgt tccagagtca tgcggatagg cggaaccaga acaacggctt
    15061 cgtcagtacc ggaagcacgc atgttggtca gtttcttacc ggtcaggcag tttacagtca
    15121 ggtcgttaga gcggctatga ataccgataa tctgaccttc gtaaacttct gcaccgtgac
    15181 cgaggaacag cttaccgcga tcctgcagac cgaacagcgc gaacgcgacc gctttaccct
    15241 gaccgttaga gatcagtacg ccgttctgac gctgacccac ttcacccgga cgtacgtcgt
    15301 cgtagtggct gaaggtggag tacagcagac cagtaccgga agtcatggtc atgaactcag
    15361 aacggaagcc aatcagacca cggcttggga tcacgtagtc gagacgtacg cggcctttac
    15421 cgtctggatt catgtttttc aggtcgcctt tacgttcgcc cagcgcctgc attacagaac
    15481 cctgatgctg ttcttcaacg tccagagtca cgttttcata tggctcttgt ttacgaccgt
    15541 cgatttcacg gaagataact ttcggacggg ataccgccag ttcgaaacct tcacgacgca
    15601 tgttttcgat cagaacagac aggtgtagtt cgccacgacc agaaacgcgg aacgcatcag
    15661 cgtcttcggt ttcttctacg cgcagcgcaa cgttgtgtac cagttctttg ttcagacgat
    15721 ccaggatctg acgagacgtt acgaacttac cttctttacc gcagaacggc gaggtgttaa
    15781 cgcagaagaa catagaaacg gtcggctcat caacggagag tgccggcagc gcttcaacgt
    15841 tttgcgtgtc gcaaacggtg tcagaaatgt tcagttcgcc aaggcccgtg atcgcaacga
    15901 tatcgccagc ttccgccagg tcggtttcca tacgttccag accgaggtgg cccagcactt
    15961 taccgacttt cgcgttgcgg gttttgcctt cgctatcgat gatagtgacc tgctggttcg
    16021 gcttcacttt accgcgcttg atgcggccaa tgccgataac gccaacatag ctgttgtaat
    16081 cgagctgaga aatctgcatc tggaacggac cgtcaaggtc aacgtccggc gcaggaacgt
    16141 ggtcaacaat cgcctggtac agcggggtca tgtcttccgc catatcttcg tggtccagac
    16201 ccgcgatacc gttcagcgca gaagcgtaaa cgatcgggaa gtccagctgc tcgtcggtcg
    16261 cgtcgaggtt aacgaacaga tcgaatacct gatccacaac ccaatcagga cgcgcgccag
    16321 ggcggtcaac tttgttgata acaacaatcg gcttcaggcc gtaagcaaac gcttttttgg
    16381 ttacgaagcg cgtttgcggc atcgggccgt caaatgcgtc aaccaccagc agcactgagt
    16441 ctaccatgga cattacacgt tcaacttcac caccgaagtc ggcgtgcccc ggggtatcaa
    16501 cgatgttgat acggtaatca ttccatttga tagcggtgtt tttcgcgagg atggtaatcc
    16561 cacgctcttt ctccaaatcg ttggagtcca tcacgcgctc ttgggtttcg gcacgagagt
    16621 cgaacgtacc ggattgttgg agcagcttgt ctaccagggt ggttttacca tggtctacgt
    16681 gcgcgatgat ggcgatatta cgcaattttt cgatcacaac tttgcctcag gcattagaaa
    16741 tagcgcgtta ttgtacacgg atcaatcgca ctacaaaaca ggatcacaaa catcctccgc
    16801 aaacaagtat tgcagagtcc ctttgtgatc gctttcacgg agcataaaaa gggttatcca
    16861 aaggtcattg caccatcatg gtgcttaatg tttccattga agcactatat tggtgcaaca
    16921 ttcacatcgt ggtgcagccc ttttgcacga tggtgcgcat gataacgcct tttaggggca
    16981 atttaaaagt tggcacagat ttcgctttat cttttttacg gcgacacggc caaaataatt
    17041 gcagatttcg ttaccacgac gaccatgacc aatccaggag agttaaagta tgtccgctga
    17101 acacgtactg acgatgctga acgagcacga agtgaagttt gttgatttgc gcttcaccga
    17161 tactaaaggt aaagaacagc acgtcactat ccctgctcat caggtgaatg ctgaattctt
    17221 cgaagaaggc aaaatgtttg acggctcctc gattggcggc tggaaaggca ttaacgagtc
    17281 cgacatggtg ctgatgccag acgcatccac cgcagtgatt gacccgttct tcgccgactc
    17341 caccctgatt atccgttgcg acatccttga acctggcacc ctgcaaggct atgaccgtga
    17401 cccgcgctcc attgcgaagc gcgccgaaga ttacctgcgt tccactggca ttgccgacac
    17461 cgtactgttc gggccagaac ctgaattctt cctgttcgat gacatccgtt tcggatcatc
    17521 tatctccggt tcccacgttg ctatcgacga tatcgaaggc gcatggaact cctccaccca
    17581 atacgaaggt ggtaacaaag gtcaccgtcc ggcagtgaaa ggcggttact tcccggttcc
    17641 accggtagac tcggctcagg atattcgttc tgaaatgtgt ctggtgatgg aacagatggg
    17701 tctggtggtt gaagcccatc accacgaagt agcgactgct ggtcagaacg aagtggctac
    17761 ccgcttcaat accatgacca aaaaagctga cgaaattcag atctacaaat atgttgtgca
    17821 caacgtagcg caccgcttcg gtaaaaccgc gacctttatg ccaaaaccga tgttcggtga
    17881 taacggctcc ggtatgcact gccacatgtc tctgtctaaa aacggcgtta acctgttcgc
    17941 aggcgacaaa tacgcaggtc tgtctgagca ggcgctgtac tacattggcg gcgtaatcaa
    18001 acacgctaaa gcgattaacg ccctggcaaa cccgaccacc aactcttata agcgtctggt
    18061 cccgggctat gaagcaccgg taatgctggc ttactctgcg cgtaaccgtt ctgcgtctat
    18121 ccgtattccg gtggtttctt ctccgaaagc acgtcgtatc gaagtacgtt tcccggatcc
    18181 ggcagctaac ccgtacctgt gctttgctgc cctgctgatg gccggtcttg atggtatcaa
    18241 gaacaagatc catccgggcg aagccatgga caaaaacctg tatgacctgc cgccagaaga
    18301 agcgaaagag atcccacagg ttgcaggctc tctggaagaa gcactgaacg aactggatct
    18361 ggaccgcgag ttcctgaaag ccggtggcgt gttcactgac gaagcaattg atgcgtacat
    18421 cgctctgcgt cgcgaagaag atgaccgcgt gcgtatgact ccgcatccgg tagagtttga
    18481 gctgtactac agcgtctaat agttgaagtt gtactacccg gcgcaacaac gccgggattt
    18541 agttgccgtg gaaactttca gcccatctct gcatgggctt ttttctccgt caattctctg
    18601 atgcttcgcg ctttttatcc gtaaaaagct ataatgcact aaaatggtgc aacctgttca
    18661 ggagactgct ttatggcaac aggcacgcag cccgatgctg ggcagatcct caactcgctg
    18721 attaacagta ttttgttaat tgatgacaat ctggcgatcc attacgccaa ccccgccgcg
    18781 caacaactgc tcgcccaaag ctcccgcaaa ttgtttggta caccgttacc ggaactgttg
    18841 agctacttct cattaaatat cgagctgatg caagaaagtc tggaggcggg gcaaggtttt
    18901 accgataacg aagtgacgct ggtcatcgac gggcgctcgc atatcctttc tgtgacggcc
    18961 cagcgtatgc cggacggcat gatcctgctg gagatggctc cgatggataa ccagcgccgc
    19021 ttaagtcagg aacagctaca gcacgcccag caggttgctg cccgtgattt agtgcgcggc
    19081 ctggcacatg agattaaaaa tccgcttggc ggtttacgtg gcgcggcgca gctactcagc
    19141 aaagcgttac ctgacccatc actactcgaa tataccaaag tgattatcga acaggcggac
    19201 cggctgcgaa atctggtcga ccgtctgttg gggccgcagc tgcccggtac gcgcgttacc
    19261 gaaagtattc acaaagtagc tgaacgcgtg gtaacgctgg tgtcgatgga actgccggac
    19321 aacgtgcggt tgattcgtga ttacgatccc agcctgccgg aactggcgca cgacccggat
    19381 caaattgaac aggtcttgct gaatattgtg cgcaatgcgc tacaggcgct ggggccggag
    19441 ggtggtgaaa tcattctgcg tacccgcacc gcgtttcaac tgaccttaca cggcgagcgc
    19501 taccggctgg cggcgcggat tgatgtggaa gataacgggc cgggcattcc gcctcatttg
    19561 caggatacgc tgttttaccc gatggtcagc ggccgcgaag gtggcaccgg gcttggctta
    19621 tccatcgctc gtaatttgat tgatcagcat tcaggcaaaa ttgaatttac cagttggcca
    19681 gggcataccg agttctcggt ttacctgcct atcaggaaat aaaggtgacg tttatgcaac
    19741 gagggatagt ctgggtagtc gatgacgata gttccatccg ttgggtgctt gaacgtgcgc
    19801 tcgctggggc aggtttaacc tgtacgacgt ttgagaacgg cgcagaagtg ctggaggcgc
    19861 tggcgagcaa aacgccggat gtgctgcttt cagatatccg tatgccggga atggacgggc
    19921 tggcgctgct caagcagatt aaacagcgcc atccaatgct tccggtcatc attatgaccg
    19981 cacattccga tctggatgct gccgtcagcg cctatcaaca aggggcgttt gattatctgc
    20041 ccaaaccgtt tgatatcgac gaagcagtgg cgctggttga gcgcgctatc agtcattacc
    20101 aggaacagca gcagccgcgt aatattcagc ttaacggccc aacgaccgat atcatcggcg
    20161 aagcgccagc catgcaggac gtgttccgta ttatcggtcg gctttcgcgt tcttctatta
    20221 gcgtgctgat taacggcgaa tccggcaccg gtaaagaact ggtcgctcat gccctgcatc
    20281 gccacagtcc gcgcgccaaa gcgccgttta tcgcgctgaa tatggcagct atcccaaaag
    20341 atttgatcga atcagaactg tttggccacg agaaaggcgc gtttactggc gcgaatacca
    20401 ttcgtcaggg gcgttttgaa caggccgatg gcggtacatt attcctcgac gaaattggtg
    20461 atatgccgct ggatgtgcag acgcgtttgc tgcgcgtgct ggcagacggt cagttttacc
    20521 gcgttggcgg ctatgcgccg gtgaaagtgg atgtgcggat tatcgctgcc actcaccaga
    20581 atctcgaaca gcgagtgcag gaaggtaagt tccgtgagga tctgttccac cgcctgaacg
    20641 ttatccgcgt tcatctgccg ccgctgcgcg aacgtcggga agatattccc cgtctggcgc
    20701 gccatttttt acaggttgcc gcgcgcgaac tgggcgtaga agcgaagtta ctgcatccgg
    20761 aaaccgaagc tgctctgacg cgtctggcgt ggccaggcaa cgtgcgccag ctggaaaaca
    20821 cctgccgctg gctaacggtg atggccgccg ggcaggaagt gttgattcag gatttgcccg
    20881 gcgaactgtt tgaatcaacg gttgcggaga gtacttcgca aatgcaaccg gacagctggg
    20941 cgacgcttct tgcgcagtgg gcagacagag cgctgcgttc cggtcatcaa aatctgcttt
    21001 ccgaagcgca gccagagctg gagcggacgt tactgacgac cgcgttgcga catacgcagg
    21061 ggcataaaca ggaagcggcg cggctactcg gctggggccg caacaccctg acgcgtaagt
    21121 taaaagagct ggggatggag tgattcacag cttgtgtgta aagattgatt attgagcgca
    21181 aattgctggt attttacgct ttactgttcc gataagttca gtatgatctt gcccggaaaa
    21241 tggggagagt cattatgctg gaatcaataa ttaatctggt atcgagtggc gcagttgaca
    21301 gccacacacc gcaaactgcc gttgctgccg tgctgtgtgc cgcaatgatt gggctgttta
    21361 gttgagtttc agattgctga tgaagtgctc tttgttcatg ccggatgcgg cgtgaacgcc
    21421 ttatccggcc tacaaaagca atattttcaa caggttttgt aggcctgata agcgtagcgc
    21481 atcaggcaat cttgttctca gcacattgat ggcgctttcg ttttctactt tgtaaacgaa
    21541 gcgccattca ctatttaaat cacccgtgag aactgctgca tccgcgcttt ctggcgcaga
    21601 taggtatcaa agcacatgca aatgttgcgg atcagcaagc gacctttcgc cgtcacctgt
    21661 attcccttct catccacatc caccagccca tcttttgcta acggggcgag cagcttgaga
    21721 tcttccgcaa agtaatcagc gaagtgcaaa tcccactgtt tctcaatagg ggcgtaatcc
    21781 agacggaagt tgcagatgag cgacttaatc acatcgcggc gaatacagtc atcacgcgtt
    21841 agcgcaatac cacgccacag cgcattgcct tgttcatcca cttgctgata gtactgcttc
    21901 aactctttct ggttctgcgc gtagcagtcg ccaatcatgc tgatggcgga aacgcccatc
    21961 cccagcagat cggtatcgcc ctgagtggtg tagccctgga agttacgatg cagcacgcct
    22021 tcacgctggg ccaccgccag ctcgtcatcc ggacgggcaa agtgatccat accgataaac
    22081 tgatagcccg attgcgtcag gaaggcgatg gtttcctgca ggatatcgag tttttgctgc
    22141 ggactcggca ggtcagcatc tttgattttg cgctgagcag caaaaatggt cggcagatgc
    22201 gcgtagttaa agacactcag acgatcgggg ttcagctccg ccacacgttt cagggtaaag
    22261 gcgaaactct ccggcgtctg tttcggcagg ccgtaaatca ggtcgatgtt ggtggaggta
    22321 aaaccaatct cacgcgcatg gttaagcagt gcaaagatga actcttcatc ctgctcgcgg
    22381 ttaaccagac gttgcacttc tttgttgaag tcctgcacgc ccatgctcag gcgattaaag
    22441 ccttcggcgc gtaaatgatc gagtacatcc agttcgattt cccgcggatc gacttcgatc
    22501 gaaatctccg catcggcatt gaactggaag ttttcgcgca gcagcttcat caggcggctg
    22561 atttgcgctt tattcagata cgtcggcgtt ccgccgcccc agtgcaattg gctgacgtga
    22621 cgcccggcga acagcggtgc acgatggacg atttcttgct ccagcgcgtc cagatactga
    22681 tcggccttgt gctgctggcg agtaacaatc ttattgcaac cgcagaagta acaaagctta
    22741 tggcagaacg ggatatgtac gtagagagat aatggacgct caggatagcg cgccacggct
    22801 tgtaaaaacg cctgttcgcc gaagtcttct gaaaactcca gcgcggtcgg gtacgaggtg
    22861 tatcgtggcc cggaatagtt atatttctgg atcagggcca gatcccagtc gatttgctgt
    22921 acagacatgc tcactccttc cgatggcgtc tctggcggct acggcgtacc ggcggatgcc
    22981 cgtttcgggt catcagccgg ttgcgcagcc acttttgtcg ccgcgacaac cgctgtagtt
    23041 taacgaataa ccacaccaga taacatataa ccggaagggt tatcagcaga acgggaaggc
    23101 ccacggcggc aatccgttag ttgccccgca gcagacgcat catgtcttct tgcttctcgt
    23161 cttcttcctc ttcttcgtca tcgtcataag agaggccgag tttctgcatc aactcatcaa
    23221 tacgatccag tttagcatcc acccaggatt gctcttcggc actcagggtt tcgcctgctt
    23281 ccagacgttc cagcagcgca tccagacgct catccgtttc cagtaattcc aactccgcct
    23341 gcggtgaaag cataggtttc tcactcttcg gtttgtgctg tttggtgact ttttcagtca
    23401 cgcccaatgg aataggggtt ttactgccaa tacgtggatc ttttggtgcg ttctggcctt
    23461 tgctgcctga cgtggtgttt ccgcccgctg cgcggctgcc cggcgcatga ccacgacgtt
    23521 ttttctgacg cttgcgatca cgagcttcct gatccagctc ctcgcgtgtt tttcgacgca
    23581 cttttgcatg acctttgctg cgtgagtttg aagatgatgg tttcataatg gttcgtcttt
    23641 tagccgtttt attcagtata gatttgcggc ggaatctaac agaaagcaag caaagaaaaa
    23701 aggcgacaga ttactctgtc gccttttttc ctgactcata acccttaacg ggtcttacaa
    23761 tccttgcagg ccgccaacac accctgtttt tcctttagcc ggaacaatcc gtgttgattc
    23821 catttccgtt taattacgtc tccggacgtt tgtcttcctg acaatcctct gtcttcgcct
    23881 cctggcgctc ctgaccctca tcctgagtca ttgttcctgt tagcgtcctc gctctatggg
    23941 tgctacttta cgcctttgct ttaaacaaac aatcaaccgc catcagtatt tgcacatttt
    24001 cagagtttta tataaaagca attcactgat ttatatagct tatttgacat gcatcgcaga
    24061 attttctctg ctatttcgca taaagactat cggcaatgtc tcacaaagcg tatggctttt
    24121 acttacaacg ctcacgccga aaaatccgcc ttttattatg gttcaggtat aatcgccaca
    24181 aagctttgac tgacggagac gaccgctttg actaatttga attatcaaca gacgcatttt
    24241 gtgatgagtg cgcctgatat tcgccaccta ccttccgata ccggaattga agtggctttt
    24301 gcaggccgtt ccaacgcagg taaatccagc gcgctgaaca cgctgactaa ccagaaaagc
    24361 ctggctcgta cctcaaaaac cccagggcgc acccagctta tcaacctgtt tgaagtggct
    24421 gacggcaagc gtctggttga cttgcctggg tacggttatg cggaagtccc ggaagagatg
    24481 aagcgcaaat ggcagcgtgc gctcggcgaa tacctcgaaa aacgtcagag cctgcaaggt
    24541 ctggtggtgc taatggatat tcgccatccg ctgaaagatt tggatcagca gatgattgag
    24601 tgggcggtag acagcaatat cgccgttctg gtgctgctga ccaaagcgga caaactggca
    24661 agcggcgcac gtaaagcgca attgaatatg gtgcgtgaag ctgtactggc gtttaacggt
    24721 gatgtgcagg ttgaaacgtt ttcttcgttg aagaaacaag gcgtggacaa gctgcggcag
    24781 aaactggata cctggtttag cgagatgcag cctgtagaag aaacgcagga cggcgaataa
    24841 ttttcttgcc ttaatgcttg tgccggatgt ggcgtatccg gcccgtaaat tcaatagatt
    24901 acaattctac cgttggcctg ataaacatag cgcatcaggc attacggatc ttttctttcg
    24961 cccaataaaa aacgccccag tcattactga ctggggcggc taaaatattc agccaaatcc
    25021 gattacgtga agtaaaaggt ctgaaagata gaacatctta cctctgtacc ctacgcgact
    25081 aactttactc ttttttgttc agttcagaaa gcactttttg taattttttt tcagttgttg
    25141 cataggaatt tctaatagcc atcacaaaac gtgacagctt atgttgctta cttacgaaaa
    25201 aagggcatgt tcaggcgaat cttagtgcgc ctgatcccag ttttcgccac tccccacttc
    25261 caccagcaac ggcacatcca gacgggtaca gttttccatc agttgatgaa tctgcttcgc
    25321 gacggcatca acatcatctt tatgaacttc aaataccagt tcatcgtgta cctgcatgat
    25381 catacgtaca cgcggttgct cagcctgtaa ccacgcatca acggcaatca tcgcccgttt
    25441 gataatgtcg gcggcggttc cctgcattgg cgcgttaatg gctgcacgtt cagccgctgc
    25501 acgacgagca ccattgctgg atttgatatc cggcagatac agacggcgtc cgtccagcgt
    25561 ttcaacgtag ccctgctctt tcgcctgagc acgggtgcgt tccatatact ccagcacgcc
    25621 agggtagcgt tcgaagtaaa ggtccatgta cttctgcgct tctttacgtg gaatgttcaa
    25681 ttgccgcgcc agaccgaaag cactcatgcc ataaatcaga ccaaagttga tcgctttcgc
    25741 gctacggcgt tgctcgctgg tgacggtttc cagtggcaaa ccaaacactt ctgccgccgt
    25801 tgcccggtgg atatcttttc cttccgcgaa tgcggtcagc aagcctttgt cacgcgaaag
    25861 atgcgccata atgcgcagtt caatctgcga gtagtccgct gagacaatca cataatcctc
    25921 tggcgcaata aacgcctggc ggatacgacg accttcttcg ttacgcaccg gaatgttttg
    25981 caggttagga tcggttgacg ataaacgtcc cgttgcagtt actgcctggt gataagaggt
    26041 atgcacacgc ccggttttcg ggttgatcat cagcggcagc ttgtcggtgt aggtcgattt
    26101 cagcttcgcc agaccacgat actccagaat cacttttggc aacggatagt ccagcgccag
    26161 ttcttccagt acctcttccg acgttgacgg cgcgccaccc ggcgttttct tcagcggttt
    26221 aatgccctgt ttttcaaaga gaatggtttg taactgcttg gtggaagaaa ggttaaattc
    26281 ctcacctgca atttcatgcg ctttcttttc cagctcagcc agacgaaggg tgagctcttc
    26341 agaatgattg tgcagcactt tcggatcgat cttcacaccg ttacgttcaa tgcgtgaaag
    26401 caccggcacc agcggcattt cgatattctc gaagacgttc aacggccctt tgtgtttttg
    26461 cagatccggc cacattttca gatgcaactg caaggtgaca tctgcatctt cggcggcgta
    26521 acgtccggct tcttcgaggg caatctggtt aaaggtcagt tgatttttgc ctttaccagc
    26581 aatctcttca aaagtgatgg ttttgtgctt caaccaacgt tccgcgaggc tgtccatatc
    26641 gtgacgcccg gcaacgctat tgagaatgta ggactccagc atggtatcaa acgcaatccc
    26701 acgcagttca atgccgtagt tcgccagaat accgcgatcg tatttcaggt tttgcccgac
    26761 cttcagcgcc ttttcatctt ccagcagcgg ttttagcaac tcgagtgcac gctcgcgaga
    26821 gatttgatcg ggcgcatcaa gataatcatg agcaaccgga atatatgccg ctacgcctgg
    26881 ctcgatagca aaagaaagcc cgaccaggtt agcagagatg ttatcaaggc tgtcggtttc
    26941 ggtatcaaat gcaaataccg gcgctttttc cagcttcgca atccacgctt tcagtgtttc
    27001 ttcatcaagg atggtgacgt agttgtcata agaaatcacc gttgccgtca cttctggtgc
    27061 ttcgtctgca acactggttt cctgtggctt cgcggctggt tttgcccctt tggcctgtaa
    27121 ccatttgccc gcttcgacat cagcagtcca gcgtttgaac tcatactttt tgaacagccc
    27181 caacaactct tccgctgccg gttgctgcac ttccagttgt tcacaggtca gctccagttc
    27241 aacgtcggtt ttaatcgtcg ccagctggta tgagagataa gcaacttctt tgttttgctc
    27301 gagcttcgct gccattgttt tcgcgccacg gaagctcaac ccagcaattt tttctggctc
    27361 ggcatacagc gtatccagcc cgcccagacc ttgcagcaat gcctgcgcgg ttttttcacc
    27421 gacgcccggt acgccaggga tgttatcgga ggagtcaccc atcagcgcca ggaaatcgat
    27481 gatcagttct ggcggcacgc cgtacttatt caccacctct tccggtccga ggatggtatt
    27541 cgtcatggta ttgataagcg taatatttgg cgtcaccagc tgcgccatat ctttatcgcc
    27601 agtgctgatc agcaccggac gcccggcttt ttcggcttcg cgcgccagag taccgataac
    27661 gtcgtccgct tctacgccag aaaccgccag cagcggcagt cccatcgctt taaccatcgc
    27721 gtgcagaggt tcgatttgtg ctcgcagatc gtccggcatt ggcgggcgat gtgatttgta
    27781 atgttcaaac agttcatcac gaaaggtttt tcccttggcg tcaaagacca ccgctgcatg
    27841 cgtcggttta tattgcatga tcagactgcg cagcatgttg aggacaccat acatcgcacc
    27901 ggtcggctcg cctgcgctgt tagtcagcgg gggaaacgcg tgatatgcgc gataaagata
    27961 agatgaacca tctacaagga taagtggatt ttgggggatc tgaaccataa tgtccgtgcc
    28021 tgtttatcag attatgggta aaggatgcca cagacaggat gaaaacatga gtttttcacc
    28081 gcttttcgtg aaaatctttt gtagatcttc tggatcgctc gcaaatcgtc atgtggataa
    28141 ctttgtgcat aaattattta catcgctttt aatttattat catgcttttt acagcatgat
    28201 gtttgtcttt gtttttcatg cagttaaaaa taaagccatc cctaataatt gagataatgg
    28261 gattttttct aatgtggata gatatgaatt atttttctcc ttaaggatca tccgttattt
    28321 gggtcgtttt ctgctaaaat ctgcgccttg cgcttcttta gcgcactgtc tatggctaac
    28381 cttttgaata aattcattat gacgagaata ctcgctgcga taaccctttt gctgagtatc
    28441 gtattgacca ttttggtcac tattttttgt tccgtcccga tcatcattgc cgggattgta
    28501 aaactattgc tacctgtgcc agtcatttgg cgaaaggttt cgcgtttttg tgattttatg
    28561 atgtattgct ggtgtgaagg tctggcggta ttactgcatc tgaacccaca cctgcaatgg
    28621 gaagttcacg ggctggaagg gttaagtaag aagaactggt atctgcttat ttgtaaccac
    28681 cgtagttggg cagatattgt cgtactgtgc gtgctgtttc gtaagcacat tccgatgaat
    28741 aaatatttcc tcaagcaaca gctggcctgg gtgccatttc ttggcctggc gtgctgggcg
    28801 ctggatatgc cgtttatgaa gcgttattcc cgcgcttatt tattacgtca tccggagcga
    28861 cgtggtaaag atgttgaaac cactcggcgt tcttgtgaaa agtttcgcct gcatcccacc
    28921 accattgtca atttcgttga aggctcccgt ttcacgcaag aaaaacatca gcaaacccac
    28981 tccacttttc aaaacttgtt gccaccaaag gcggcaggca ttgcgatggc gctaaatgta
    29041 ctgggtaaac aattcgataa actgcttaat gttacgctgt gttatccgga caataaccgt
    29101 cagccgttct tcgatatgtt aagcggtaaa ctgacgcgaa ttgtcgttca tgtggattta
    29161 cagcctattg ccgatgagtt acatggcgac tacatcaatg acaaaagctt taagcgccat
    29221 tttcagcaat ggctgaactc actgtggcag gaaaaagacc gacttctgac gtcgttgatg
    29281 tcatcacagc ggcaggacaa gtaaattttg ctcgcctctg aatgagcaga ggcgagtgag
    29341 tattttaatg aacaaaacgt ccggcacgag acataaattc ttcttcgctc atctcctgtc
    29401 cgttaaaaac aactttaccc ggtgtataac gcagttgcag cgaggcggta ttgttgtcaa
    29461 tcgtgattaa ctgaaacatc tgacctaagg tcatcatccc gctgatttgt ttatcagccc
    29521 gcttttgcgc tttttccgca tccattcctt cagataaatt aagctgtttt gcggtttctg
    29581 tgacgacatt aagcggtaac ttcacatcaa aattgagcga tttaatatct ttgtttgtgg
    29641 atgatgaaga tttggctggg tcggcaatac tgatatccag attggcattt agttcgccga
    29701 gtgcgttctt ccatcttacc ggttgtttaa tggtcggctc acttttttgt aataacggta
    29761 aatactcttt gaacaatgca gcattcacat cttgcagagc aacttcatcg tttgctaact
    29821 cagggtgtac agcaagctgc ttctgcatag caatgttata ctggatgata aattggcgca
    29881 ctgctgaggg atcaatagat tcagcaatca atgaccactc accactaccg agttgctgat
    29941 tcccttttgt tagcttatca agttcgtagg tcagattgac attgatataa tctttgtctt
    30001 taacgcgatc cagcatggtt tttgcaacga aagcatcgat ttgggcaacg tcttccccgt
    30061 gattgataat gttaatctga ttaaatttcg actcgctttc accaaccgga aatttctttt
    30121 cttctaatcg cgccagagaa tcaatggtaa aactttttgc cgttactttg ttcattggtg
    30181 ataattgcag aacaatatct tcgactttgc cttcaatatc caggttagac aatgaactac
    30241 catcaccatt aaaagtaaac tggccctcac caaaagaaaa ctttccatac tctgcagggg
    30301 caacatctaa tgtcgtagag aatgagccac caaacgcgaa tcgcaatgtc gcttccacgg
    30361 gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag ttcattattc actaagttca
    30421 cttttgccgc tgccagtgct gggatgacat taccatgcat taacattgtg atgggcaacg
    30481 gaccgtgctc aacatccaca tcaaatacaa ctttctggcc tggcttgata ttgagatcgg
    30541 gtgcaccgtt atcgaatgtc atttgcattt gaaaacgaga attaaaaaaa cctttgtcaa
    30601 aatttttata acttaacgtc atatcgtagg catgttcacc tttcttttta gcatcgttaa
    30661 aatctttaat aaacttttcg acaccaggct gaatttgcgt acctgtgtac catgtgccac
    30721 caccccagat tacggctaac gcaacaataa cacctgtagc tgacttacgt atcatataaa
    30781 tattccctga taaaacagca aaactttaaa tatccgctta tgctgaaata atgtccagcg
    30841 gcaggacgca ttatcagaat gtatttatat ttattaaata ataaaaaaac ccgtgaatat
    30901 tcacgggcct tatgtaattt acattgaatt attttttctc ggacagatat ttcactgtat
    30961 cagcatactg ctgaacaaaa acatccatat tgctagtatc cataccctgc ggattcagct
    31021 gatatttacc gttaacaaac atcgccggaa cgccacgcaa ttgcacgtca gctgcagctt
    31081 tttcctgctg agcgaccaga gatttgacca cgaagctgtt ccacgccgcg tcgtactctt
    31141 cacctttaat acctgcgttg ataaatacat cgcggatatc agatgctgaa cgaatggtct
    31201 gggttttctg tacgccttca aacagcggaa cagtgacttt gtcttccacg cccagcgcca
    31261 tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg aagttgacgt
    31321 ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca gaaatatgca
    31381 gaacttcttc aaactgatag cagtgcggac agaagaaaga gaaaaactcc agcacttgcg
    31441 gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca tactgcgccg
    31501 ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt ttcatgatct
    31561 actctctccg attaatacat aggtgttaat tgtaaagggg gttcttgtag aacttttgcc
    31621 tgttctataa aagtcgccgt ttgtcgcagc cagtaatctt ccccggttaa ccacgggaaa
    31681 tttttcggga acgcgggatc agcccaacgc cgcattagcc aggcaagata ataaaccaaa
    31741 cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc gctaaattct
    31801 tcataagctt caataatagt ttccagttgc atccgctgct cggctttatc gccattgagc
    31861 aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc aacaaacatt
    31921 ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg cagaacggtg
    31981 aaatcttccc gccagtgcgc tgtaacggcg gcgatcagct catctgtcgc tttcaggaat
    32041 gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg tggctcaatg
    32101 agatattcat tcagaccgat ggtcgggcga tggataaaaa gctgtttgcg ccccgtctgg
    32161 tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc agcttcgaac
    32221 tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt caataaagtc
    32281 tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccaa ctgcaacgca
    32341 aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata aaatttgacg
    32401 acaaaacgtc gacgatcttc gtcctgaaat tgatagacac ggttttcata gctgttaagc
    32461 ggggtaagac cggaatccac ccggatccct tgctcaaata gagcatccat gatggtatcc
    32521 gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg aaaacattac
    32581 gaatgatgaa ggatatcatt tcacgatgat tttggtggca ccgcttacaa gcttttactc
    32641 tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat aatctttctg
    32701 caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat agatcagaat
    32761 gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca gtttctgcaa
    32821 aaattgcaac aggttaaggt ctggctcttt ttgccaggct ggctggagga gttcaataac
    32881 ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag acacgttagc
    32941 agggtcaatc ccacaataaa agaggcgata tcggtgaatc tgatgacgac gataacaggc
    33001 gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg attgctggaa
    33061 ttaaacggca aaccattatg gcaacatgtc gctgacgcgc ttatggcgca gctctctcac
    33121 gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caagcggtct gaaagtgatt
    33181 gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt aatgcagcag
    33241 gaagcaggcg agtggttttt gttttgtccg tgcgatactc cttatattcc cgaggattta
    33301 gcagcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca cgacggtgaa
    33361 cgcgatcacc cgactattgc tctggtaaac cgcgcaattg agcctttatt actggaatat
    33421 ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg tcatgcggtt
    33481 gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga gcttgcccga
    33541 tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt accggaaaaa
    33601 ctacgctttt gaaacaactg atcccggcat tatgcgccag agggatccgt ccagggctga
    33661 ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc tatgagctgc
    33721 gcaaggctgg cgcggcacaa accatcgttg ccagccagca acgatgggcc ttgatgacgg
    33781 aaacaccaga cgaagaagag ctggatctac agtttctcgc aagtcgaatg gatacctcaa
    33841 agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag attgtgctgt
    33901 ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat gttattgctg
    33961 tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat gatgttgagg
    34021 ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca agagtaggct
    34081 ggataagacg cgccagcgtc gcatccggca tttttctgca ccagcgcaaa aaggcccagt
    34141 cttccgaccg ggcct
//
LOCUS       DAZKIV010000030        33958 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZKIV010000030 DAZKIV010000000
VERSION     DAZKIV010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.030"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..1370)
                     /gene="codA"
                     /locus_tag="ACHOT6_001521"
     CDS             complement(87..1370)
                     /gene="codA"
                     /locus_tag="ACHOT6_001521"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFP9946762.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(1360..2619)
                     /gene="codB"
                     /locus_tag="ACHOT6_001522"
     CDS             complement(1360..2619)
                     /gene="codB"
                     /locus_tag="ACHOT6_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFP9946763.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(2958..4844)
                     /gene="prpE"
                     /locus_tag="ACHOT6_001523"
     CDS             complement(2958..4844)
                     /gene="prpE"
                     /locus_tag="ACHOT6_001523"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFP9946764.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(4884..6335)
                     /gene="prpD"
                     /locus_tag="ACHOT6_001524"
     CDS             complement(4884..6335)
                     /gene="prpD"
                     /locus_tag="ACHOT6_001524"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFP9946765.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(6369..7538)
                     /gene="prpC"
                     /locus_tag="ACHOT6_001525"
     CDS             complement(6369..7538)
                     /gene="prpC"
                     /locus_tag="ACHOT6_001525"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFP9946766.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNELHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETVDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYYIADRLETVMWETKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(7583..8473)
                     /gene="prpB"
                     /locus_tag="ACHOT6_001526"
     CDS             complement(7583..8473)
                     /gene="prpB"
                     /locus_tag="ACHOT6_001526"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFP9946767.1"
                     /translation="MSLHSPGKAFRGALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEAKLDDLFARSQVK"
     gene            8711..10297
                     /gene="prpR"
                     /locus_tag="ACHOT6_001527"
     CDS             8711..10297
                     /gene="prpR"
                     /locus_tag="ACHOT6_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFP9946768.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDVRQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCKLEEDMQQGRFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(10395..10670)
                     /gene="yahO"
                     /locus_tag="ACHOT6_001528"
     CDS             complement(10395..10670)
                     /gene="yahO"
                     /locus_tag="ACHOT6_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFP9946769.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            10817..11488
                     /gene="yahN"
                     /locus_tag="ACHOT6_001529"
     CDS             10817..11488
                     /gene="yahN"
                     /locus_tag="ACHOT6_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFP9946770.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASIIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(11505..11750)
                     /gene="yahM"
                     /locus_tag="ACHOT6_001530"
     CDS             complement(11505..11750)
                     /gene="yahM"
                     /locus_tag="ACHOT6_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFP9946771.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(12162..12977)
                     /gene="yahL"
                     /locus_tag="ACHOT6_001531"
     CDS             complement(12162..12977)
                     /gene="yahL"
                     /locus_tag="ACHOT6_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFP9946772.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIHP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(13220..14269)
                     /gene="yahK"
                     /locus_tag="ACHOT6_001532"
     CDS             complement(13220..14269)
                     /gene="yahK"
                     /locus_tag="ACHOT6_001532"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFP9946773.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHVKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(14356..15312)
                     /locus_tag="ACHOT6_001533"
     CDS             complement(14356..15312)
                     /locus_tag="ACHOT6_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946774.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(15309..16280)
                     /locus_tag="ACHOT6_001534"
     CDS             complement(15309..16280)
                     /locus_tag="ACHOT6_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946775.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRGPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(16273..17757)
                     /locus_tag="ACHOT6_001535"
     CDS             complement(16273..17757)
                     /locus_tag="ACHOT6_001535"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFP9946776.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EVGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(17806..18789)
                     /locus_tag="ACHOT6_001536"
     CDS             complement(17806..18789)
                     /locus_tag="ACHOT6_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9946777.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(19043..19453)
                     /locus_tag="ACHOT6_001537"
     CDS             complement(19043..19453)
                     /locus_tag="ACHOT6_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFP9946778.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(19752..21134)
                     /gene="yahJ"
                     /locus_tag="ACHOT6_001538"
     CDS             complement(19752..21134)
                     /gene="yahJ"
                     /locus_tag="ACHOT6_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9946779.1"
                     /translation="MKENNSRREFLSQSGKMVTAAALFGTSVPLAHAAVSGTTHCDAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQNGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(21144..22094)
                     /gene="yahI"
                     /locus_tag="ACHOT6_001539"
     CDS             complement(21144..22094)
                     /gene="yahI"
                     /locus_tag="ACHOT6_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFP9946780.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSDSQRDELQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAHEIHADILVITTGVEKVCIHFGKPEQQALDRVDNATMTRYMQEGHFPPGSML
                     PKIIASLAFLEQGGKEVIITTPECLPAALRGETGTHLIKT"
     gene            complement(22237..23655)
                     /gene="yahG"
                     /locus_tag="ACHOT6_001540"
     CDS             complement(22237..23655)
                     /gene="yahG"
                     /locus_tag="ACHOT6_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9946781.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEAADLAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEKQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(23655..25202)
                     /gene="fdrA"
                     /locus_tag="ACHOT6_001541"
     CDS             complement(23655..25202)
                     /gene="fdrA"
                     /locus_tag="ACHOT6_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9946782.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKSGADNEQLLAEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGTKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLKDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(25192..26055)
                     /gene="yahE"
                     /locus_tag="ACHOT6_001542"
     CDS             complement(25192..26055)
                     /gene="yahE"
                     /locus_tag="ACHOT6_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9946783.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWKQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMMGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEIIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(26095..26700)
                     /gene="yahD"
                     /locus_tag="ACHOT6_001543"
     CDS             complement(26095..26700)
                     /gene="yahD"
                     /locus_tag="ACHOT6_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFP9946784.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            26958..27455
                     /gene="yahC"
                     /locus_tag="ACHOT6_001544"
     CDS             26958..27455
                     /gene="yahC"
                     /locus_tag="ACHOT6_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9946785.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGISGFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            27547..28479
                     /gene="yahB"
                     /locus_tag="ACHOT6_001545"
     CDS             27547..28479
                     /gene="yahB"
                     /locus_tag="ACHOT6_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9946786.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(28521..29609)
                     /gene="pdeL"
                     /locus_tag="ACHOT6_001546"
     CDS             complement(28521..29609)
                     /gene="pdeL"
                     /locus_tag="ACHOT6_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9946787.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDQSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(29751..33734)
                     /gene="ehaA"
                     /locus_tag="ACHOT6_001547"
                     /pseudo
     CDS             complement(29751..33734)
                     /gene="ehaA"
                     /locus_tag="ACHOT6_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
BASE COUNT         8141 a         9209 c         8179 g         8429 t
ORIGIN      
        1 ttcagtgctc tacgtaggcc ggataaggcg ttcacgccgc atccggcata aactacgctc
       61 gctttgtcaa cacacagccc agccgatcac cgtttgtaat cgatggcttc tggctgctcc
      121 agatatacgg tggtttgtgc cggttgtgtg ctggcaatca ccttgccgcc acgtaccgaa
      181 taacgtaccg gaacctgacg gcgcagcgca tcaaacccat tttcagccgg caggataatc
      241 aggttggcgc tgtttccggc ggcaatgccg taatcctgca aattcaacgt ccttgcgctg
      301 tggtgggtga ttaaattcag gccatcgtta atctgcccgt agcccatcaa ctggcaaaca
      361 tgcagcccca tatgcagcac ttgcagcata ttcgccgttc ccagcggata ccacggatcg
      421 aagacatcat cgtgaccaaa gcagacgtta atgccggact ccagcatctc tttaacgcgc
      481 gtgatgccgc gacgttttgg atacgtatcg aaacgtcctt gcagatgaat attgaccagc
      541 gggttggcga caaagttaat accggacatt ttcagcaagc ggaacaggcg tgaggtatac
      601 gccccgttat aggagtgcat tgccgtggtg tggctggcgg tgactcgcgc gcccatgcct
      661 tcacggtgcg ccagggcagc aacggtttcg acaaagcgcg actgctcgtc atcgatctca
      721 tcacagtgaa cgtcgatgag acggtcgtat ttttgcgcca gggcgaaggt tttatgcagc
      781 gactccacgc cgtattcacg ggtaaattca aaatgcggaa tcgcccccac tacatctgcc
      841 cctaagcgta acgcctcttc cagcaacgct tcaccgttgg gatacgacaa aatcccttcc
      901 tgagggaagg cgacaatttg cagatcaatc cacggcgcga cttcctgctt cacttccagc
      961 attgctttca gcgcagttag cgttgcatcc gaaacatcga catgggtacg cacatgctga
     1021 atgccgttgg caatctgcca tttcagcgtt tgccaggcgc gttgtttcac atcgtcatgg
     1081 gttaataacg ctttgcgctc ggcccagcgt tcaatgcctt caaacagcgt gccggactga
     1141 ttccagttcg gttgtccggc ggtttgcgtg gtgtccaggt gaatatgcgg ctccacaaac
     1201 ggcggtataa ctaaaccttg ttcggcatcc aggctgtttt cagttatggg catcacgccg
     1261 gattgcgcat caatggcgct gatttttccg tcctgcagat gaatctgcca cagcccctct
     1321 ttgcctggta accgggcgtt aataattgtt tgtaaagcgt tattcgacac tgttaacctc
     1381 cacatgcgtc attgctgctg tcgttttacg attcaaaatc gggttaagga tcagatagct
     1441 cagcgcgcca cctaataccg cgttgaccgg aacaattccc ggtaaccagt ggcctgcggc
     1501 aatccccagc gcgaccgcca gaatcgccac ccaattgaca ctcatcatac gcgtggtcgc
     1561 aaagtgctca tagcggcgac ggttcatcag atagtcggcg atgatcacgc cccctaccgg
     1621 aggaatagct gccgaaagga aggtcaacca gccgacaaaa ttgttataca gccataatgc
     1681 gcagaccgta ccgataatac cgttgattac cgaaagggtt ttgctcgaca taccggtaat
     1741 gttggcgaaa cctaaacccg acgcatagag tgcgttatcg ttggtggtcc agatattcag
     1801 ccccagcacc acaatcgcag gcagcagcag gccctgagca atcatcacat cagagatatc
     1861 cgccatgccc agtgccgcag cgcccgctgc accgaaaata aacatcaacg agttgccgag
     1921 gaaaaaggcc accatcgcca ccagcaccgc cagtttggca ttgcgaccaa accggacaaa
     1981 gtcagcggtg agcgtacccg cactgataaa tgaccccaca accagcgcca gcgcgacatt
     2041 gaaatctaac ggttgtgcgg gaacgaccgc ttttaatgcg tccaggccgc ccatgccgtt
     2101 aacagccagc cacacggaat aaccgcccag gcaggcgata gccggaaccg caatcaccga
     2161 aagaaccgtc agcgccgaaa tgccaaaaaa gacggtgacg gtcatcagta aaccggaaac
     2221 ggcaatcagc aaattaatat ccagcccggt tgccttaccc accggaatgg caaacatcgc
     2281 cacaccgacg ccaaaccagc caacctgagt tccgcccagt agcagtgaag gcagccatga
     2341 gcctttaaca ccaaacgaga agcgagcaag aagatgagtg gtcaggccgg tttttgcgcc
     2401 aatgtaaccg agaaatgaag tgtaaatacc gaggagaaga ttaccgatga gaactgcgag
     2461 gaagaaatca tgatagctaa gaccggttcc gagagtgccg ccggtccaca tactggcgga
     2521 aaagaaggtt aatcccagca tgacgaacgt caatgccaat acccctttcc gcgccgactg
     2581 cgggactggc ccctggctaa agttgttatc ttgcgacacg aaattccccc tttttgctgt
     2641 ttgaaaccca aaaaatccgc cgcattctat tcatctgaag ataaaaagca atcgttttcg
     2701 tggggaaata tattttttat atgaatgagt gcaaaaaggt gggggaaaaa ttatcggtgt
     2761 aaaaacagtg ggtagtgggt agatactgat aagacgcgtc agcgtcgcat caggcatcgg
     2821 cgcggggtgt cggatgcgac gcgagcgcct tatccgacct acggttcagg cctgtaggca
     2881 taataagacg cggtaagcgt cgcatcaggc atcggcgcat ggtgtcggat gcgccacgcg
     2941 acgcatccga caatacctta ctcttccatc gcctggcgga tctgatccaa tgacgcagga
     3001 tcatcaatgg tcgtcagatc cccaggatcg cgtccttcgc aaattgcctg gatcgtgcgg
     3061 cgcagcattt ttccggatcg cgttttcggc aattgcgaga caaaccagac gtgcgccggg
     3121 cgaccaaagt tgccaatctg gctgtccacc agcgccataa tcgccttctc ttgcgaatgc
     3181 gccacatcac gatcttccag actatcgctc tctttcggaa tgacaaacgc caccgccacc
     3241 tgccctttca gcgcatcttt caccccaacc accgccactt cggcaacgcc cggatggctg
     3301 gagatactct cttcaatctc gcgcgtgccc agacgatgcc cggcaacgtt aattacatcg
     3361 tcagtgcgcc cgagaataaa gtgataaccg tcagcgtcac ggatgcccca gtcaaaggtg
     3421 gcgtacaccg ggcgggaaaa cagcgaccag taagtcttca caaagcggtc gtcgtcgccc
     3481 cagatggtct gaatacagcc cggcggcagc ggcccttcca ccaccagcat ccctttctcg
     3541 ttgacgccac acggttcgcc ggtgacttca ttaagcaact gcacgttata gccatacatc
     3601 ggcacgccgg ggcttcccag acgcgtcggc ctgtcgtcca gaccgcgagc aatcgccata
     3661 atcggccagc cggattcggt ctgccagtag ttgtcgatga ccggcacatc cagcgtattg
     3721 ctcacccaac tggcggtcgg ctcgtccagc ggttctccag ccagatagag cacttccagc
     3781 gacgagagat cgtgtttgcg aatttcagcg gtagggaatt ttttcagcac gcgaatggcg
     3841 gtcggcgctg agaacatccg gctaacctga tatttctcga cgattttcca ccacacgccg
     3901 cagtccggcc aggtcggcaa tccttcgtaa acgatagtcg ccatccccgc cagcagcggc
     3961 gcgtagacga tatacgaatg ccccaccacc cagccgatat ccgatgcgca aaagaacacg
     4021 ctgcccgctt tgccgccaaa aatggtgtcc atcgaggtcg ccagcgccac cgcatatccg
     4081 ccgacgtcac gctgcacgcc tttaggtttg ccggtcgtgc cggaagtgta gagaatgcag
     4141 gaggtttcgt tggattccag ccacgccacc ggtacccgcg cgccgatatg ttgatggcgc
     4201 aacgacgcga aatcgacatc ccgcccgctg acgcgcgcca ttttcgccag cccgcgatcc
     4261 accagcaaaa catggcgtgg ctggtgctgc gcctgactta tcgcatcgtc gagcaatttt
     4321 ttatagggaa tgattttgcc accgcgcgct ccggcatcag ccgagacaat cagcaccggt
     4381 ttagcgtcat caattcgcgc cgccacgctg tgcgaggcaa aaccaccaaa taccaccgaa
     4441 tgaatagcac caatgcgcgc gcaggccagc agagtaatat gcgcttcggc aatcatcggc
     4501 atatacacca gcacccgatc gccgcgctgc acgcccagtg agcgcaacat tgaggccacc
     4561 gcgttcactt cgtcatgcag ctggcgaaag gtaaaggtgc gctcttcttc tgtttccgaa
     4621 gagacggcaa tcaatgccag tgcctctggc tgtttctcca gccagcggtc gatagcgttg
     4681 tggcacaaat tggttcggcc ttcacaaaac caacgggcaa acggcggatt gctgtggtcg
     4741 agcgtttgcg taaagggcgt ctgccagtca atacgccggg cctgctcggc ccagaactgc
     4801 tccggttcgt taatcgaacg ctgataaaat tcgctaaaag acataatgct ctcctgttga
     4861 acttacgcct taccgccgtt tacttaaatg acgtacaggt cgagatactc attgaccggc
     4921 atctgttcca ggcgagttct gtcgagagaa acctccagaa tgcgctgctg ttggcgagtc
     4981 gggaactggc gcgcgagatt gattttgaat ttatcgacca gtttcggaat accatcctga
     5041 cggcggcgag catgaccaat ggggtactcc accaccactt cttcaaatcg tgtgccgtcg
     5101 gtgaactcaa gggtaatggc attggcgatg gcgcgttttt ccgggtcgtg gtagtcagcg
     5161 gtaaatgccg gatcttcaaa gcaattgatc ttctcgcgca gggcgtcgat gcgtttatct
     5221 tgcgcaacgt tgtcctcgta atctgccgcc gttaagcgcc cgaataacag cgggatcgcc
     5281 accatgtact gaatgcagtg gtcgcggtca gccgggttat tgagcggccc ctttttgtcg
     5341 atgatgcgaa tacaggcttc gtgggtgcga atggtcactt tttcgatatc cgccgccgtc
     5401 ttgcctgctg cctgcatctg ttcatagagc gtcatcgccg cttcaactgc cgtctgggag
     5461 tggaactccg ccgggaagga gattttgaac agcacattct ccatgacgta agaaccgtac
     5521 ggacgctgga agcggaacga ttcaccttta aaggagacgt cgtagaagcc ccataccggc
     5581 gcggtcaggg ctgacgggta acccatttcg cccgttttcg ccatcagtgc cagacgtacc
     5641 gcgcgggaag tggcatcgcc cgccgcccag gatttacgcg tgccggtgtt cggcgcatgg
     5701 cgataggtgc gcagcgactg accgtccacc cacgccagcg aaacggcgtt gagaatttcc
     5761 tcgcgggtca ggccgagcat ttcggcgacc acggcggtgg aagccacttt cactaacaga
     5821 acgtggtcga ggccgacgcg gttaaaggaa ttctccaacg cgatgcagcc ctgaatttca
     5881 tgggctttga tcattgcggt cagcacctgt ttcatggtca acggcgcttt gccgctggcg
     5941 accgcgttgc gcgaaagcca gtccgccgtt gccagaatgc cgccgaggtt gtcggaagga
     6001 tgcccccact ccgccgccag ccaggtatcg ttgaaatcga gccagcggat catcgcgcca
     6061 atgttaaatg ccgcctggac agggtcgagc tgaaactgag ttccggggac gcgcacgccg
     6121 ttgggtacga cggtgccggg gacaattggc cccagaagtt ttttacaggc tgggtattcg
     6181 agagcttcca gaccgcagcc gagcgtgtcg agcaggcagt aatgtgcggt gtcgtaggcc
     6241 actttagaac tgatttcgta gttcatgacg taatcgacga tatcaacgat ttcacgatca
     6301 aattccgggc ggatgttgtt gatttgagct gacattgggt acgtttcctt attgttattc
     6361 gtagaggttt actggcgctt atccagcgcg acaaactggc ggtcttccgg tccaacataa
     6421 ttggcggaag gacggataat tttgttgtcc tgacgttgtt cgataatgtg cgccgcccag
     6481 ccggtgacgc gggcgataac aaacagtggt gtgaacatct cggtgggaac gcccatcatg
     6541 ttgtaggaaa cagcggagaa ccagtcgaga ttggggaaca tctttttcgt ttcccacatc
     6601 accgtttcca ggcgatcggc gatgtagtac atcttcaacg agccgccttc ctgcgagagc
     6661 tgcttcgcca cacgtttgat cacctggtgg cgcgggtcgg cgatggtata aaccggatga
     6721 ccaaaaccaa tgaccacttc tttgttttcc acccgcttgc ggatatcggc ttcggcttcg
     6781 tccggcgttt cgtagcgttg ctggatctcc agcgacactt cattcgctcc gccgtgtttc
     6841 ggcccgcgca gtgcgccaat cgcgccaata atcgcggaat acatatcaga gccagtgccc
     6901 gcaatcaccc gactggtaaa ggtggaggcg ttaaactcgt gttcggcgta cagcactagc
     6961 gaaatatgca tcgccttttc ccagctttgc gatggctttt cgccgtgcag cagatgcagg
     7021 aagtgaccac cgatggagtc gtcaacggtt tccggctgga tgcgttcgcc gttgtgactg
     7081 tagtgatacc aataaaggag aatcgagcta agtgatgcca gtagtttgtc ggcaatatcc
     7141 cgcgcgccag agacggtatg tccctctttt tctggcagcg tgcagccgag cgcggaaacg
     7201 ccggtgcgca taacatccat cgggtgtgac gccgccggta aggcttccag cacggtacgc
     7261 acgttagccg gtaaaccgcg cagggctttc agtttcgttt tgtaagcggc gagttcgtca
     7321 cgggtcggca gtttgccgtg gatcagcaga tgcgccactt cttcaaattc gcaatgttcc
     7381 gccagatcaa gaatatcgta gccgcggtaa tgcagttcat tgccgctttt acccacggtg
     7441 cagagcgccg tattgcccgc cggaacgcca gaaagtgcca cagatttttt cggtttaatg
     7501 acatgggtac tgttttgcag gatcgttgtg tcgctcatgt tgtcctcgtc atttgtaggg
     7561 tcgagatatt tttcgttgtg ttttatttca cctggctacg ggcaaacagg tcgtcgagct
     7621 tcgcttcgta ctggtagtag ttgatgcttt cgtacagctc gttgcgggtt tgcatggtat
     7681 cgatgacgct tttctgcgtg ccttcctggc gcaggacgtt gtagacatgc tcagcggcgc
     7741 ggttcatggc gcggaacgct gaaagtgggt acagcgccat tgcgacatgg gcgctgcgta
     7801 attcgtcggt ggtaaacagc ggtgttgcgc caaattcggt aatgttggcg aggatcggca
     7861 cctgcaccgc atcggcaaac tggcggtaca tggcgagttc ggtaatcgcc tccgggaaca
     7921 acatctcggc acccgcttca acataggcct gtgcacgctc gatcgccgca tccagcccct
     7981 ctaccgccag cgcatcggtg cgcgccatga tcacaaaatc aggatcggtt ttcgcatcca
     8041 ccgccgcgcg gatccgatcc accatctcct ctttcgagac gatcgctttg ttcgggcgat
     8101 gaccgcagcg tttcgcacca acctgatctt caatatgcaa tcctgccgca ccggctttaa
     8161 tcatcgattt caccgtacgc gccacgttaa aggccgaaga accaaaaccg atatccgcat
     8221 ccaccagcag tggcagcgaa caaacgtcgg tgatacggcg aatatctgtc agcacatcat
     8281 caagggtaga aataccgaga tcgggcagcc ccagtgaccc tgccgccacg ccaccgccgg
     8341 agagataaat tgcctgatat ccggcacgct gcgccaacag cgcatgatta gcgttgatgg
     8401 tgccaacaat ctgcaatgga ttttctttag tcagtgcacc gcgaaacgct ttacctggag
     8461 agtgtagaga catagcccat cctttgttat caacttgtta ttttcgttaa taaaacaaag
     8521 caaggggtgt gccacaattg ccttaataaa attaaatgtt taatttcaat ttgttagaga
     8581 taacgtcatg aactaatccg caaatatgcg tttcagttaa cgtttcaggc aatgtttcac
     8641 cgcgtttcat tgcaacaatt atgaaacaag actaaaccca atattcggtt tcttaacttt
     8701 gcggtgcgct atggcacatc caccacggct taatgacgac aaaccggtta tctggacggt
     8761 atctgtaacg cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg atcacctggc
     8821 gaacattacc cctattcagc ttggctttga aaaagcagtg acctacatcc gcaagaaact
     8881 ggcaaacgaa cgctgtgacg ccatcatcgc tgctggctct aacggcgcgt acctgaaaag
     8941 ccgcctgtca gtgccagtta ttttgattaa accgagcggc tacgatgtgt tacaggcact
     9001 ggcaaaagcc ggaaaactca cctcttctat cggcgttgtc acctatcagg aaaccattcc
     9061 agcgctggtg gcgtttcaaa aaacctttaa tctgcgcctc gatcaacgta gctacattac
     9121 cgaagaagac gcccgcgggc agattaacga gctgaaagct aacggcactg aagcggtggt
     9181 cggcgcgggg ctgattaccg atctggcaga agaagccgga atgaccggaa tttttatcta
     9241 ttccgccgcc accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc gcatgtcgtt
     9301 acgccataac actcacgatg ccacccgcaa cgccctgaga actcgttacg tgctgggcga
     9361 tatgctcggt caatcaccac agatggaaca agtacggcag actattttgc tgtatgcccg
     9421 ctccagtgca gcggtgttga ttgaggggga aacggggacg ggcaaagagc tggcggccca
     9481 ggcgattcat cgggaatatt ttgcccgcca cgatgtgcga cagggcaaaa agtcgcatcc
     9541 gtttgttgcc gtcaactgcg gggcgatagc cgaatcgctg ctggaagcgg agctgtttgg
     9601 ctatgaggaa ggggcgttta ccggctcgcg acgcggaggt cgcgccgggc tgttcgaaat
     9661 tgcccacggc ggtacgctgt ttctggatga gattggcgaa atgccgctac ctttgcagac
     9721 tcgcctgtta cgggtgctgg aagaaaaaga ggtcacccgc gtcggcgggc atcagcctgt
     9781 tccggtagat gtacgggtca ttagcgccac tcactgcaag ctggaagaag atatgcagca
     9841 aggacgtttt cgccgtgatc tgttttatcg gctgagtatt ttgcgtctgc aattgccacc
     9901 actgcgcgag cgggtggcgg atattcttcc gctggcggaa agctttttga aagtgtctct
     9961 ggcggcgctc tccgccccgt tttctgccgc attacgccag ggattacagg caagcgaaac
    10021 cgtgctggtg cactacgact ggccaggtaa tattcgtgaa ctgcgcaata tgatggagcg
    10081 actggcgcta tttttaagtg tggaaccgac gccggattta acgccgcaat ttttgcagct
    10141 gctactgccg gaactggcgc gcgagtcggc gaaaactccc gctccacgct tactgacacc
    10201 acaacaggca ctggagaaat ttaatggcga taaaacagca gcggcgaatt atttaggcat
    10261 cagccggacg acgttctggc ggcggctgaa aagctgaact gccgaatgca gcgcgaacgc
    10321 tttatccggc ataaatttca atagatgatc accgataaac ttacgcatcg ggcatgttta
    10381 catagggttc agaattactt cttcttataa atatttgccg tgccgtggat cttattgtca
    10441 gtttgaccgg aggtcagtac caacacatca gccccttttt catccgcttt tttgatcaaa
    10501 tcttctttcg cgtctgcagt cgacatttca ttgctggttg aaatatcacc tattttttca
    10561 tactgcgatt caactttttc aaattccgct ttggtcatca attcagcggc atagacattg
    10621 gtaacggcta acgctaacgc accgactaac attttagaga ttattttcat attacggctc
    10681 cagagttgtt atcacactat cacccaccca cattattggg ttgattaata attagttcag
    10741 tcagggcata aacgccagta accggaaata agacactttg tgatctctct cgcacccgat
    10801 tatttttggt agaaatatga tgaagttact tcacttattt atggatgaaa tcactatgga
    10861 tcctttgcac gccgtttacc tgaccgtagg actgttcgtg attacttttt ttaatccggg
    10921 agccaatctc tttgtggtag tacaaaccag cctggcttcc ggtcgacgcg caggggtgct
    10981 gaccgggctg ggcgtggcgc tgggcgatgc attttattcc gggttgggtt tgtttggtct
    11041 tgcaacgcta attacgcagt gtgaggagat tttttcgctt atcagaatcg tcggcggcgc
    11101 ttatctctta tggtttgcgt ggtgcagcat gcgccgccag tcaacaccgc aaatgagcac
    11161 actacaacaa ccgattagcg ccccctggta tgtctttttt cgccgcggat taattaccga
    11221 tctctctaac ccgcaaaccg ttttattttt tatcagtatt ttctcagtaa cattaaatgc
    11281 cgaaacacca acatgggcac gtttaatggc ctgggcgggg attgtgctcg catcaattat
    11341 ctggcgagtt tttcttagtc aggcgttttc tttgcccgct gtgcgtcgtg cttatgggcg
    11401 tatgcaacgc gttgccagtc gggttattgg tgcaattatt ggtgtattcg cgctacgcct
    11461 gatttacgaa ggggtgacgc agcggtgagc agataattta ctctctacgt aatcaacctg
    11521 atttgccgaa tcccggccat cggcgatgat agcccccgta atgcgtggtc cttatttccg
    11581 ataagtttac ttgagtcttt agcttcccat ttcagcccac tctcccctgt gtgggctttt
    11641 ttcattggtc tgacccgtcc tgaatagcca aacaatggca atgttccact ttgcagggac
    11701 gataataagg ggatctgatt cagaagagtt ttgaaaagtt ggaccgccat cgaggcctcg
    11761 aaccccgtac catacaacaa tcctggttgt cgtctgctct tcctgatgag ctaatggcgg
    11821 tttttgatat gagccttaca gcatattttc tgtcaggctt gagtgcgatg cgtaagatac
    11881 agaaaagacc ccacacaaac atgatggaat tatgattttt ttcatatgaa tgtcaaccca
    11941 cacattcaca aaacatctgc atgagatcat tttttataca ccgcatggct gcgaaaccac
    12001 tgctggtgct tttttaagcg aagtgtcgcc gatcacaaca aaatatcggc gaaaaggcgg
    12061 tggaatgcca tttggcatac ggatatacag atgaaatgat tacctgcttt gcgattcact
    12121 ctctggtgga ataacaactt gcaggtggaa ccctttctga actaaagagt gtagcggatt
    12181 ttaacgtgct tatttacagg ataatatctg tcacctaatt cctgcactct attcgatatc
    12241 cattcattta atcgtgcgtc atctctgtat gtttttacgt ccagaacaga gagcaataac
    12301 atcaccagtg attcaggaaa aacagggtgg atatttttta agtgtaataa taatccatgc
    12361 gcaagcgcct gcttaatggg cgcgtctgtc tccggaatag acgacatttc gcgaagccat
    12421 ttttccacac gcatacactc tttcccttta gggaaaattc tcagcaaagg catatggtgt
    12481 tcaagaagaa tacatttgca atctgttcgc actgaatcga taaactgcac taatttctta
    12541 agcccggtaa agcgtccagc ctgcacattt gctttaatat caagaaacgc atcatcctgg
    12601 tcatataccc aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa cttaaccggg
    12661 tgatcagggt aatccatcca gtcataaaat cctgggaaat cattaaactg cacatttatg
    12721 gtgagtgcgt tatcgtctga ccactcacag tctcgtacgg cagccatgag atgaatgagt
    12781 ttgtcgtcat cgactaaatc atcacgacaa tcataaggat aatcataata ttgtttaatc
    12841 aaaaccttga atatatcgac acaggaaacc agggagcttt tgatatcctc tggtaactga
    12901 ttgtttttaa ccgtattaag tatgctttgt tgcgtatatg gcatcaaatt attgtgcgga
    12961 gcttttaatg atatcatcgc gatctctctt aaataaaaat aaacgtcgca gataataaag
    13021 cgctgattaa accaggcact atcagaaatc gctcataact aacgtgtgaa gtatagtgta
    13081 ctggagggag ttaatttaac aatttgaatt tacgttatag gttttactgc tactttgata
    13141 ttatttcgct ctccttaact aaaaagttaa gtggagagcg aagttaatat gttaaaccac
    13201 agcgtattta ttaatttttt cagtctgtta gtgtgcgatt atcgataaca aaacgatatt
    13261 tcacatcacc acgcagcatt cgctcatagg cttcattaat ttgatcggcc cgaatcatct
    13321 ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag catctcctga gtttctggaa
    13381 tgccgccaat catagaaccg gctatcgcac ggcgtttcat gatcaggttg aaaacttccg
    13441 gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca cgcttcagca
    13501 aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt attcaaaatg aaatcgaaac
    13561 tcttcacatg agccgccatc tcatcggcat tgcgtgagtt aacaacttca tcggccccca
    13621 gggcttttgc cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca tgcgccccca
    13681 tcgcgtgggc cagcttgatc cccatatgtc ccagaccgcc gatgccgacc acgcccactt
    13741 ttttacccgg cccggcctgc cagtgacgta gcggcgaata cgtggtgatc cctgcacaca
    13801 acaaaggagc caccgccgcc agctgctctt gcgggtgacg aatacgcaga acatatcgct
    13861 catgaacgac gatctgttgt gagtagccgc ccagagtatg gcccggttcg tccggcgtcg
    13921 gcgagttata ggtgccggtc atgtgatcac agtagttttc cagcccgtct tcacactctt
    13981 cgcaatgttt acaactgtcg acaatgcagc cgacaccgac cagatcgccc ggcgcatgtt
    14041 tttctacctg atcaccaacg gctaccacac gccccacaat ttcatgaccc ggtacgcagg
    14101 ggtaaaccgt ccccgcccac tcggaacgga cctggtggag atcggaatgg cagacgccac
    14161 agtaagcgat ttcgattttg acatcattcg gtcccggttc acgccgtgtg atatccatcg
    14221 gttcaagtgg ttgtttagcg gaatatgcac caacagcttt gatcttcatt gtgtttactc
    14281 ctgattagct atgtgtattt ggcgatgcct gatatgtcat cgcggcaaaa cgcgtccatt
    14341 gaatataacc aatatttaaa ccacccgatc cagccccaac atgccgcgaa cctgaatgaa
    14401 acagagcagc aacgttcccc ataaggccat cgtcaggtag gggcttatac cgagaatatt
    14461 gaagccgctt tcaataattt gcagaagaaa cagcgccagc accataccaa ttatccgccc
    14521 gctgccgccg tccggattaa ccccaccgag tactgccgcg aggattgaca ccagcaagta
    14581 cgattcgcca taagaggcct tagccgagtt gagtttcgac atcatcaaaa atgccgctac
    14641 cgcacaaagc aaggcggaaa tgacataaac ccaaatcagc acccgacgag tgttaatgcc
    14701 ggagtaaagt gttgctcgtt cgttagaccc aatcaggtaa atggctttac cgagcggcgt
    14761 tttctccagc aatatccata gccccagtgc cactgcggca aacaaccaca tcggtagcgg
    14821 aataccgaac cattgcgcat gattcaacca cagcacccat gagggataat tggcaatggc
    14881 gctaccacct gtcaccagaa tatttatccc tttgagcaac gtcatcatcc caagcgttgc
    14941 aagaattggc gacactcgaa taccagcaat caatacgccg ttacataaac cgattatcac
    15001 cgccgcgcca gcgcccgcca gtaacgtagc gacaaccgta gcaatacccg gtggatactg
    15061 agtcgccacc cacgccatca ccagtgaaca ggcgttagca gtggctataa ttgacaagtt
    15121 aatgccaccg caaagcatgg tcaccgccat agccaacgcc agaatgccca gcacgggcat
    15181 ttgcgaagcg acggactgga aattacttat cgaccagaaa atctccggca tagtaatgct
    15241 gaacgcggca acagttagca ccagcaggcc gatcaagtaa aactcaacgt tattacgcca
    15301 cgattttttc atagcagact ccggttctga tgctgactcc acgccgtggc actaatgctg
    15361 gcaacgatga tgatgccggt gatcaatgtt tgccagtaag acgagactcc caataaattt
    15421 agcccgtttt gcatcactgc caacaacacc acgccgagca aagtaccggt taacgtgccg
    15481 cgcccgccga gcaaactagt gccgccaagc accaccgcag ccagtactgt cagctcataa
    15541 cccagaagag aatcgggggc gacagtcatc accgtccacg actgcactac acccgcagcg
    15601 ccagacatca atcccatata gccatagacg aaaagttgca gtttcagcac gctaaagccg
    15661 atgcgactgg ctgattcgcg gttcccgcca agggcataaa ttttgcgccc tacactggtg
    15721 aaattcatga taaacgctgt tagcagcacc accgtaatca tggcgatcag cggcagacca
    15781 aggccatagt catagccatc agcatcggtg tacttgaaca acataacccc ctgctcaaac
    15841 cacggcggaa agtcgtacag ccacacacct ttactcaacc ataacagcag gccatagaaa
    15901 atattcagcg tcgagatagt gatgatgata gaaggccccc gtaggcgatt aaccagtaag
    15961 gcgttaatta agccaagtaa aatgccgatg cagcccgcca gtgcaaacgc gacagcgaag
    16021 ttgccaccaa ttttttgcag caacagcacc atgccgtatt gcgcaatgat ggtcattgct
    16081 ggaaacgaaa tatcaattcc accggaaatc aacaccacaa acaaaccaca ggcgagaata
    16141 gtcaacatgg catagttatt ggcgagatcg tacaaattac cgaatgtcag aaactcgtcg
    16201 ctgcgccagg caaggccaac aaacagcacc acaatcagta atgccagcca gaattcatgg
    16261 cgttttttca gttcagccat ttaccacctc cgcaatcctg gcctgagaac tgctgtcagg
    16321 caggaagcta tgggtgatct gacctttttg cattaccaaa atccggtggc tttgatacca
    16381 cgcttcttca atttcatcgc agatcatcaa taccgcaatg ccatgagccg ccagatcgct
    16441 gataatgccg tatattcctg ctttgttggc gatatcaaca ccgactgtcg ggctatcgag
    16501 aatcaacagt cgtggcccaa tcgccagcca tttggcgatc gatactcgct gcgcgttacc
    16561 accagaaagc gtattcaccg gaagatgagg atcagagact ttgatggtca atgccttgat
    16621 tagcctgtcc accaggtcac gaactttagt ttcactcaga aagccgaagc gatcttttac
    16681 cttgtgaaaa acagtgctaa tgatgttgtc ttcaatagat tgcgccatca ccaaaccgcg
    16741 cgacatgcga tcttccgaaa cataaccaat accgtggcgg atagcgtcct ggttactatg
    16801 cagtgtaacc ggctggccgt tgatgaggat ctcgccagca tccggtcggg tcataccaaa
    16861 cagactcaga cataattccg tgcgccccgc accaagcagg ccaacaatcg ataccacttc
    16921 gcccacttcc acccgcaagt taatatttag atattcccca tgacgactta aattacgcac
    16981 ctccagcacc ggggtgctgg attttccctg ccacaactct cgcacctgat attcaaactc
    17041 ctggccggtc atcaggaaac cgagttgttt tgtggtcatc tctgcagccg gaaaagtccc
    17101 gaccagctca ccatctttca gcactgaaat acggtcggaa acttccatca cttcttccag
    17161 acgatgactg acgaagacca cgcagatccc gcgttcacgc aattgatgca ccacctgtaa
    17221 taacccttgc acttcctgat gggtcagcga agcggttggt tcatccatca cgatcaaccg
    17281 tgcgtcctgc gccagtgcac ggcaaatagc caccagctga cagcgggcaa ttgaaagctc
    17341 agaaacgagt gtatccagtg gcaatgtgac gttaatactg gtcattgccg cctgcgccac
    17401 ctcacgcaga cgacggcggt taacaaacag accgtggtga tagtgattca cggcgatatt
    17461 ctcccagaca ctcaggttag gaaataaaga gaggtcctga taaatcacct gaatcccctg
    17521 cgccaccgaa gcggcgggtg tcagctttga ccagtttttc ccaccaagcg taatttccgc
    17581 tccctcatcc gggcgataaa caccggacat aattttaatc aacgtcgatt taccacagcc
    17641 gttttgcccg gcaaggcagt gcacttcacc cgacataaag tcgagagaaa catcgcgcag
    17701 cgctcgcgtg gcatgaaacg ttttgttgat atgacgaagg gaaaggaaag tttccataac
    17761 atcccctggc aataagaatc ggggctttcg ccccgaagaa atggattaat aaagtgagtc
    17821 gatattgtct ttgttgacca gtaatacctt atggaaacgg ataattcgct tatccatatc
    17881 tacgtcggcc ttacccagat tctgcatttc taatccaggt ttgatctctt cacccttcag
    17941 caacgtgctg gcgacggctg cgagtgcgta acctgcagag gccggatcgt aagtaatccc
    18001 ttcggtgata tcaccacttt taatcagtga tgccgcctgt gaagggatca tcatgccata
    18061 gactgcgact ttatttttcg cccgtttctc tttcaccgca cgtcccgcgc caatcggacc
    18121 gtttgaacca aaggagacaa ccgctttcaa gtcaggatag gttttcatca ggtccagtgt
    18181 ggtacgacgt gagtcatcca cactctcggc aaccggcatg cggcgggtaa cttcatgcat
    18241 atccgggtaa tgctctttct ggtatttcac cagcaagtca gcccataagt tatgctgcgg
    18301 cacggtcaaa ctacccacgt aaatcacata gccgcccttg ccccccatgc gtttcgccat
    18361 atgctcaaca tattcagcgg caaatttttc gttatcaatg atttcaatat cccagttagc
    18421 acttggctga ccgggggatt cgttggtcag aaccacaatt ccggcatctc gcgctttttt
    18481 gaataccggt tccagcacgt tggcatcgtt tggcacgata gtaattgcat taaccttacg
    18541 ggcgattaaa tcctcaataa ttttaacttg ttgcggagca tcagtacttg aaggccccac
    18601 ctgtgaggca ttaacaccaa aggctttacc cgcctcaacc acaccttcgc ccatgcgatt
    18661 aaaccacggc ataccatcga ctttagaaat attcaccacg actttttccg ctgcctggag
    18721 cggcgcagaa attagcgcag cgcctaataa cagcgaagac accatattga taacaaaacg
    18781 tttattcatc atttcagcct cattgcaggg tagtcaggaa gtgttcgccc aaattagcgc
    18841 gctggagaga ataagaattt tcgaatattt ttatgcagaa taaacagccc tatttcaggc
    18901 agctattaaa aatatggtta taaatatgga caaaacgggc agcaacaata agtgtgacat
    18961 tcatcacgta aaaaatagca aaataaacaa aattagcgat attttatgtg tgtgagttta
    19021 tttcgcgtag ataaatagtt atctacacaa cgtcaataag ggcatattct tcaaatataa
    19081 cgtccatttc tggtgaatag cttccttctg cctggaaaaa acgacggtgt tcattacgcc
    19141 aatattcaag actgaggtca ccttcacctt ccttacgggc aagctccacg gtgacatcag
    19201 aaaagcgcag taaatgcagg ccaatcactc tgataacaca tacaggctca ccacggctat
    19261 tttctacaat cttatactca ccaatcatcg gaaatgcgtt gtcttcaata catcccgcca
    19321 gcgaaccaca ggaagctgtt ttaacaccag tcgcaatcaa cgttgcgagg tgatcggcca
    19381 acgctgctga atcgccaaaa gtacaaaagg aagcatgggg atatttttgt gagaattttt
    19441 cttttatggt catggcaata tttttgcgat tacggaaaaa caaatcacat cttaattact
    19501 gttggtaacc atcagcaaga gtatcattgg tttttcgata ctgaatcttt tttatccgaa
    19561 gctggtcaca tttatggcct gataaatacc tttgttaaaa ctcacttttg tgtatttctc
    19621 tcaaataatc ccctggaaat tatgccgcga gattataaag ttcaggatca ttcattagca
    19681 aaccattccc gccccactcc ctggggctta aatctcttac aattcaccgt tgcaggcgtt
    19741 tcaaatgcgc atcaacctgc cacactcccc cacaccagtt gccctttatg gaacgttgcg
    19801 gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggcgtccac cagcacaaag
    19861 ctggcatcat cctgcgcttt tggccataca cgctcgcctt tttcattcag cggcaataca
    19921 tcgccagtgg ctagaaacag cgaacgggag aggttctgtt cgttaggacg aatatagagc
    19981 tgcgcgtaga gattggcttt ttccagcatg tcgcccagac cataaggcga ccagtggtcg
    20041 ataacgctgt cagtgccagt catcactttt acgcctttgt cgtgcaactg tttgagcggc
    20101 atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc cgccatccga
    20161 ttcgccagcg catcgacctg ttgttcgttg agcgtagcca gcgcaaaggc gtggctgatg
    20221 gtcaacttac ctttcagttg cggcgttttc tctaccgttt caaccatata attgatggct
    20281 gccacgcccg acggagtggt ttcgtgcagg tgaatatcga cacctttgtc gtagtccagc
    20341 gcaatctgaa acatggtgtc gagggatttt tccatcgcgc catcaacact ggtcgggtcc
    20401 agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc gcattaaggg ttccgatttc
    20461 gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc cgcctgacgt
    20521 cgcgccagca ccgcctgcaa attctgcaga tttttcaggc cggaaaccgg ttcgatattg
    20581 cagtggctgc gggcaatggt ggtgcctttc gactgcaata gatcgatcag tttttctgcc
    20641 cgttcctgcg tgtacggttg cagttccggc agcattttct gctcaagctt gatcatgtcc
    20701 tggatggtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta gaaggttttg
    20761 tcgagatgaa tatgcatatc gcgagtggtg ggcagcatca gcttaccgcc agcatcatag
    20821 tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcaacaat tttgccgttt
    20881 tgaatctcca cggtctggcg cgccgtgcgg gtctgtaccg ccacaccgtt ttcgtagtca
    20941 aaaccggttt ccagtagcac gttgtcgaga tagtaatgcg ggtcagtgat tttcatggtg
    21001 ttattcgcgt cgcagtgggt tgtgccagag actgccgcat gggcgagcgg cacagaggta
    21061 ccaaacagcg cggcagcggt gaccatctta ccgctctggc tcagaaattc acggcggcta
    21121 ttgttttctt tcatcttacg tccttacgtt ttaataaggt gggtgcccgt ttcaccacgc
    21181 agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc ctgttccagg
    21241 aacgccaggc tggcgatgat ttttggcaac atgctgcccg gcgggaaatg cccttcctgc
    21301 atatagcggg tcatggtggc attatctacg cgatccagcg cctgctgctc gggtttgcca
    21361 aaatgaatac acactttttc caccccggtg gtgatcacca gaatgtcggc gtgaatttca
    21421 tgggccagca gcgcggtaga gagatctttg tcgataaccg cgtccacgct ttggtaatcc
    21481 cccgcttcag tacgcactac cggaattccg ccaccgcccg cgccaatcac cacaaagcct
    21541 tgttggatca gcgccttaat ggcgggtgct tcgacaatac gtttcggttc cggcgaggcg
    21601 accacgcggc gatagccccg cccggcatct tcaacaaaac accagtcagg gtttgccttt
    21661 tgtaattcgt cacgctggct gtcgctaaag aatgcgccga tgggcttggt gggatgggca
    21721 aatcccggat cgtttttatc cacttccacc tgagtcacca cggtgacggc ttttttctcg
    21781 ccgtgacgcg ccagtcggtt gttcagcgcc tgttggatca gatagccaat gccgccttgc
    21841 gtatccgcta cacagttcgc cagcggcgtt aatggcagcc cttcgcgctc gtgggcaatc
    21901 tccgcacggc gtaaatccag cccgacctgc ggcccgttgc cgtgggtcag cacaatgtcg
    21961 taatcggaag ccagcatttc cagcaccgta tcggcgacgg ctttcaccgc ctccgcctga
    22021 tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat ggcaacgacc
    22081 acaagttctt tcatgttgtt atgtccgcta cagagtgtga tggggttatg accaacgcca
    22141 gactgcctga tgcgctacgc ttatcaggcc tacaattctc gggccatccg taggccggat
    22201 aaggcgttca cgccgcatcc ggcatgaaca tgtaggtcag acgccgtaac gttcgcacca
    22261 gccaagaatg gctttctcga agcaggcaaa aggtggatgc acaatgcccg cgccaatcat
    22321 gccgacgccc gcatctttat gggcgatggc agtgttgatc accggcagaa tgccgctgct
    22381 acccacgcgg gtgatgtcga ttgccgacgg cacgcccata aaaccgagca gcggaatggt
    22441 gacgttgggg ttttcaccga gagtgatttc acgcatctgg cgagagaaat cgatagcctc
    22501 ttccaccgtg ccgcccacca gcgcaacgat tgcaggcgcg gtcgccatag caaatccgcc
    22561 aataccgtag gtttcagtga tggcgctgtc gccgatatcc agccccgaat cttccggctt
    22621 ataaccggca aacatcgggc cgatgacctg ctgcgccgga ccggtaaacc attgccccgg
    22681 taatccgctg acccgcaggc cgaactcgac gccgttacgc gccatggtgg tgaccacggt
    22741 gctgtattcg atgccgtgcg ccgcatccat cgccgcttta cacatcgcca tccacgtcgg
    22801 gccggagaag tagtcgctgc tggcgacaaa ctcaaacact tcgcgctgtt tctccacgga
    22861 ataacctgcc tgaataatcc ccggcgtcag cgcctgaatc agcaacgtcg tcccggcgtt
    22921 attgcggtta tggcactcat cgcccatatg cagcgcctgc gccagcatta agcgcagatc
    22981 gatctcaccg ataattttca tcgcgtcgcg cagcattggc ccctgcacat cgcgcatcca
    23041 gttcaggcgg tcgatcacgc tctgatcgtt ggcacccata cgcaaaatct tcgccatctg
    23101 ctcgctcata ttggtgtaag cgatgttgcc gtaggttttg tttttcacga tgtgcataaa
    23161 catcgaggcc gaggtgacgc ccgccatcga tcccacacag tcgtgttcgt ggcacggcga
    23221 gaaggtgatc tccccggagg ccgccaggtc agccgcctcg tcgagatctt tcgccagtcc
    23281 ttcgaacacc agcgctccgg tgaccgcgcc tttcatcgcg ccgcacattt tttcccaggt
    23341 gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg cccggcacca cgttaatcgc
    23401 ctgatcaaaa ccaatcagca ccggatgcga ctgaataatg cgctccagcg cctgctggtt
    23461 agcggcggcg attttgtccg ccagcggcga atcggcaatg ttatccagcg cctgcaccac
    23521 ctgcatattg ccctgccccg gcggcgtcca gtcgagttgg gtcacttcta catgctgctt
    23581 tttcaggtca tcgctaaaca tggcgatgcc gacgttaata acgttcaatg gttggctaaa
    23641 cagtgactgg ctcatcaggc ttcctcccct ttgcagataa attcacgcgc cagcaatccg
    23701 gtattggtgc tgctgctcgc cagaatcact ccggcatcaa gcaacatctg gctttgttgt
    23761 tctaatgatg gcgtatcgag atcggtgccc agcacatagg cgagaatgat caactcgcgt
    23821 ccttcggcgg cggcgatcgc tttcgcttct ttgatcgcct cgatggtgga gccgaccgga
    23881 tcttcatgcg atccaaatcc gagcacaaaa tccatcacga tcaccgccac ttctggatcg
    23941 cgcgcttctt cgatcaagcg actgatgcgg ttggtggggt caatcattgg atgcggcttg
    24001 ccgttggtga agtcgtcatc gccaaagtcg aggaaggtgt gtttgatgct gcggttgata
    24061 tctttcaggc ggaattccgg atcgggctga atgttgctgt agacatcgcc atgtttttcc
    24121 atcaccgcga acatggtttc gtcgcacaac gtgccgccgc agaacaggcc acgaatgtat
    24181 ttctgctgcg gttgcagacg cgcacgcaca tccgcaatca acggctggtt aagcgtatgc
    24241 aggtcgagat tttcctgttt cacgccggag agcatcaccg cttttagcgc cgcctctttg
    24301 gtgccgcggg caaactgtag cccctgctca tccaccggcg tttcaacgcg accgaggaag
    24361 cagaccacca ccggcttgcg gcaggcacgt gcgcgttcca gcactttgcg ggccaccgca
    24421 ggtgcgggag gttttgaaat cagcgcgata atttcagttt gcggatcgtt ttccagcatc
    24481 ccgatggcgt cgagcatcat caggccgccg attttctcgc tcaggtcgcg cccgccggtg
    24541 ccaatcagtt gtgaaacgcc gccaccaaat tcatgaatgc ggacgctcag ctcctggctg
    24601 ccggtgccgg atgcgccaac aataccgatg ttgccgcgac gcacggcgtt accaaaacag
    24661 agcgccgcgc cgttgataat cgccgtgcca cagtctggcc ccatcatcag cagccctttt
    24721 tcgtgggcca actgcttgag cgccagttca tcttcaactg agacgttatc ggaaaacagc
    24781 atcacgttga gatcgttttg cagcgcctga cgcgcttcgc gagcggcaaa cagaccgttg
    24841 accgaaatca ccgccaggtt actttccggg atatgctttt tggcgctggc aatagtggcg
    24901 taacgcgcct cgtgcgagcc gctttgcgct ttgctattga acagttcttc aatctccgcc
    24961 agcaactgct cattgtccgc acccgattta ccattgatga caatcatcag gtcgccgttt
    25021 ttcgcctgtt ccagctccgg cgtcagcagt cctaaatttt tcagcacacc tttgttcatt
    25081 tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga gtttatttgc acgcgtggag
    25141 atagacatca gcgagacaga atcaaaatag gtattcggtt taatgactat tttaactgac
    25201 ataattccct ccgtaggttt ggctcagcgc acaaccatcg gccatgccat acagcatgtc
    25261 gcagccggaa ctggagccaa tatgtttaat tatttctatt gcctgagttg aattcccagg
    25321 gacgccataa ataatgttgt gaataaagcg atgaatattt tcccggcagc gttgttgtaa
    25381 tgcggcttcc agcgttatgg cacttaataa cgtggtgtta tttctgccgc gtagcagacc
    25441 gcgataaaat tcctctttgt atttttctgc cggatgcccg ggaataaata aaataagcgc
    25501 cagacctgtt aaataatcat cggctgatgg cgtaaggcca atccctaacc ccatcatact
    25561 ggcgaccgct gcggcgatat tttgcttatc gtttactgtc tgaataactg cccgtcgttt
    25621 aatatgtaat tctttattta acgcctgata aaacggatta tcgccgcgat aattaaacag
    25681 ggtttcattt tcccccaggt gctgatgaat aatgtcgtgc cactgtttcc aggcgataag
    25741 gtgaaaattc acagcggtca tttgcaaggt tggggacagc caacgccgac aacgactcat
    25801 ctctatatga agatgttgac caaccgtaat accttgatcg tcaaactgaa ccttatcacc
    25861 atgccggaac agatcatcaa agtgagtgag tgccaaccga caactgtttg gcgcattgtc
    25921 gtactcttca caaagcagcg tcagcaactg ctggcgagcc gggagtgcga tatttaccgc
    25981 tctggcaaag acctgttcaa cctgtccttg cccccgctgc gccagaaaat ccgcatccgc
    26041 agttagcgcc cacacacacc gtttttctgg tgtgttgata gcaagcctcc cggtttatgc
    26101 acctgcggca atcagtaact gcgcaatctc ttcaaagccc cgttcccgcg ccagttccag
    26161 tggcgttttg ccatatttat cggtcagatg cgggctggca ccgtgttcca gcaataactg
    26221 cacaatcgcc tgctgtttaa taccaccatc attaagcaca atcgcttcca gtagcggcgt
    26281 ccagcctaca tggttggtct ggttaacgtt aatctccgta tgcgccaaaa gctcttttac
    26341 aatacttaaa tggccttttt cacaggcagg cgtcaggccg acaccgccaa aacgggttac
    26401 gcaattaaga tcgggtttag ccggtaaaat aattcgtagt agtgttaaat catcgttcag
    26461 acagctaatt aaaaaaggat ttaaacaggt atgatcttgt ttattaatat ccgctccggc
    26521 gtcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg taattgccgt
    26581 tttcccctga cgatcgcagg tattaatatc gacacccagc gcaaggcagg tttttacttt
    26641 atcgatatca ccctgttgtg cagccaataa atagtcggca ggtagatttt ttatactcat
    26701 tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa cgataatctg
    26761 ataaaagaac ccgcttaaat ttaattcgcc gtatttcgcc tttttgttga atgatgttca
    26821 acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga taccctttca
    26881 ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttt gtccatgtct
    26941 aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc ggtatttgtg
    27001 cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg gaattattag
    27061 gaaccattgg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcggt aaatcggggt
    27121 ttatcagaag tctggcggtt aattattccg ggatggtgtg ggcgtttttc gccgcgctgg
    27181 ctgccggttg gctggcctcg gtgagtggga tttctgggtt ctgggcgagc gtgattacta
    27241 ccgtgccttt ctctgcagta gtggtctggc aagggcgttt ctggttactg tcttttattc
    27301 ccggtggctt tctcggcatg acgctgtttt ttgccagcgg gatgaactgg acggtgacgt
    27361 tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggt ggacaaaaat
    27421 taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat gattatctat
    27481 ctttaaccga tacctctaca gtcatagctg gagaaattca tagtagataa tggcgagaat
    27541 ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac cgctgcgcgt
    27601 tttggcagct tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc cattagctac
    27661 accattaagc gtatggagac ggggctggat gtggtgctgt tcactcgcag tacccgcagc
    27721 attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct gctgaatgat
    27781 tttcacgcca tcaaacgcag tatcgatact atttcgcagg gcatagaagc acgtgtgcgc
    27841 atttgtatta atcagctgct ttatacgccc aaacacaccg cacgcttgtt acaggtgctg
    27901 aaaaagcagt ttcctacctg ccagattacc gtcaccaccg aagtgtataa cggcgtctgg
    27961 gatgcgatta ttaataatca ggcgaatatc gctatcggcg cacccgacac gctgctggac
    28021 ggtggcggca ttgattacac ggagattggc gcgattcgct gggcttttgc catcgccccg
    28081 gatcacccgt tagcctttgt gccggagccg atcgccgaaa gccagctgcg tctctaccct
    28141 aatatcatgg tggaggacac cgcacatacg attaataaaa aagtgggctg gttgctgcat
    28201 gggcaggagt caattctggt gccagatttc aacaccaaat gtcagtgcca gattttaggt
    28261 gaaggaattg gttttttgcc ggattacatg gtccgtgagg cgatgacgca atccctgctg
    28321 gttacccgac aaatccataa tccgcgccag gattcgcgca tgttactggc gactcagcat
    28381 tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat ttgcgccaaa tggcatatta
    28441 acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac ctcccgatgc
    28501 gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga gataaattta
    28561 ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc tttaccgatc
    28621 attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag accaggctta
    28681 cgcgctagtt cgacaatatt gtccacaata tgaccggaga tttcgtcgac actcgccatt
    28741 tgcacaaatg actgatcgat cttaataaaa tcgaccggga acgcctgcaa gtaacgatag
    28801 gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg atgaaggctg
    28861 tcaaatatcg ctctggcttc tggcgttacc ggaatagggt tacgttccgt tagctcgaga
    28921 accagcttga ttttatcgtt acctaatttc ttaaccaggt tcagacactc tttttcaaat
    28981 cccgctgcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt gtccggcagc
    29041 aaatgtttta ccggcatcag aatatccgca gtctgtttca tcagttggcg ggtcattatg
    29101 acaataagac cggatgactc cgccagagga ataaactgat ccggtgggat aattcccgtt
    29161 tgtggatgtt cccagcggac aagtacctca cagcccgtca gtaccccagt ctgcgcgcag
    29221 aaaaccggtt ggatccacgg tttgaattcg tggttttcca acgccagact gatggcttca
    29281 ggcgtgacga tatggtgaca acggttatca gggatataaa aattattttt atttcccgtg
    29341 ccactgatga cttgcggatg atattgaaaa aagatatccc gccagaaggt aatatcactc
    29401 tggatgccta gtttttcata cagctgcttc ttttgatgtg aaattgtctt tgcactgcga
    29461 tttctgtact gtgagatttc tgtaaccgac attccctcca ttagcagctt gagtaaaagc
    29521 cgttcttttt cggacacgat accaggcaac gtcaaattca ccgttgcccc gaactcttcc
    29581 aaaaaaacac tcaaatcatg cgaagccatt gaacacacct tgattaaatc ttgtgcgcca
    29641 gctaaatctg cgagtcttca ctctgactga taaagctggc tgcaagaaaa tgacacagag
    29701 agccgaatta tctcccgttc ggctctctct ccttttactc attattcaga tcagaattgc
    29761 cacttaatgc caaccattgc agaggtgtca ttatatcccc tatcggcaac ctgaacgccc
    29821 acattccccc agacattcag gttggcattt agctgtcctt ccaccccggt ttttatctca
    29881 gcaatatttc gggctccatc ctgagtgacg gacacgccat ccatactggt gctgaaatcc
    29941 ttactgttat gcagccagtt cacttctaca aacggctgga actcgcggga tttaccgtca
    30001 tccattttat ggtggctctt cagccaggtt tttacgccaa gtcgggtttg aacattgcca
    30061 tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt gcttatcggc tttgactccc
    30121 atccaggtaa cctgtgcttg cggctgaaca taccattcat tacgcgttcc ctggctgcca
    30181 ttaaattcag ctaatttgtg tttgtatcca gcttccagtg aagcggtaaa tccttttgat
    30241 ttataggatt cactttgtaa gtcatcccct ttcactgtgt tatcaaacca gctgtactgc
    30301 gcccaactgt cgagatacgc gccattacgc gactcgtcat cggcatacca ggtggcatag
    30361 aggccggtgc tatatccgtt cacactcgct tttgcacgat aaccggttcg cgaggaaatg
    30421 gttttgctgt cgctgttgcc atatcccgcc atgaccccaa catgccagct gtcgctgccg
    30481 ttttggctcc actgcgcgac atcgcctccc tgttgcagaa catagcgatt gctttgggtt
    30541 ttcagctggc cgctgccatc acgccattta ttatgaccac cttcatggcg catccacata
    30601 gtggtttgtt tctgctcacc cgtcaccatg tcggtatagt acgtattacc cagacgctca
    30661 tgcagacgcg tggtgaacat tgtattcgct gctgcaaggt tcgcaatgta gctacccgcc
    30721 tccggtcgca ggtcattatc cacattcaga tccggacccg gcgtaggtgt cgggttaggg
    30781 ttcggctctg gattcgggtt tggctccgga ttcggcatcg ggtctggctc cggctgcagt
    30841 tcaggagaat cactgccgct ggtcagatac cagttaccac tatttgcccc ttgtccacgc
    30901 gcgagagtgt agtcatacgc ccccgcaacg atacgaccag cctgaacaaa ttcgccctca
    30961 gacttaccgt ctacatggat aagttcgata ccgttaaggg ttttcgcacc tgtaccgcca
    31021 gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc cctcgacaac caatttatcg
    31081 gttactgagt catcgccatt cagtaccgta ttcattacta gttgaccgtt attaccgtgg
    31141 tagttcccct tcaccactaa cacattgcca gcttgctgtc ctgacattcc agtcatcagc
    31201 gtaccgttgt tggtcagatt gccgcccagg gtgtaattca cagacgcagc cgctcttttt
    31261 tcagcgctat tattcaacgc cagggtaccg ttgttctcaa cgtttgcgct acccaatccc
    31321 tgcgcatgac ttaccaggac attagccgaa ttagcaatgt tcacattcgc cgtcaggttg
    31381 ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat tcagctctcc gctccccgtt
    31441 aacgaaccgg ttgaagtgcc cccgttggta accgtcagac tgccgccatt taagctcagc
    31501 agtgaatcgg cgctgccatt gagcgtgccc acggtctggc tgtgaccatt catatccagt
    31561 tcagtctctg ccgccagacg cagttcatgg gtattaccca acacgttatc gttattcaac
    31621 aaaagcgtcc cactgcgcag atcggtaaca ccggtatagt cgttgtcttt gttagaaagc
    31681 gatacggtct gacccttctg gctgctgaat gccaggtcac cactgccggt aatctttgcg
    31741 ctgagatcgg cggcattctc gcttttaccg ttagagctca gcaccagagc atcgctgtcg
    31801 gtagcgtgta aatcaagctg ggtcaggccg taaccgatat acagaccgtc attgtttacc
    31861 ccactcgtca gacgatagtc gtaagtgccg acggccgctt tttgcccatt ctgctgaata
    31921 tcacgttggg cactgctgga gaggatttgc ccatttttat cctgcaattg cagattgccc
    31981 gcatcgccca gaacttccgc accttgcgca tcaaccaact taatggtgct gttaccatca
    32041 tcgacttcag tgagcgataa cgcagagtca atatcacgag atactgagct tacaacgtca
    32101 ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca gcgttttact aacgttgacc
    32161 gtcccttctg tcatctgtgc acctgctgtc agggcaccaa aatcaacggt tccgccggag
    32221 aatcccaaag tgctaatgga gtgagcgccc ttacccactg ttaccaggct tccctgaccc
    32281 gcagtgatcc cgcctctctg cagaaactca gcattctttt ccaggttaaa tgtcgtgttc
    32341 tggaacgcca gtgaaccctg gaaccagtct gtataagcaa actcatcgaa ggagaattcg
    32401 tgattcccca tgtttatttc aacaatacct ctaccttgaa gatcgttatc aaatacccaa
    32461 tctttattgg cattgatttg cagtttgcca tcaacatcca ggtccgcacg gccgatgttc
    32521 ttgtcttcag aaataaccgc gacagcacca gaagcgacat caatatatcc gctgaatgca
    32581 tagttattgc cgatcagtgc cactgtccca ctgtcgatac ttattgtacc attgccggaa
    32641 atcttattgg caaaggtcat gctatcgtca gccttcaggt gtaatttccc gcggttgttg
    32701 atctctgctg tacctatatt ttgggtgatc cctaaagcgc cattttgctc aattttgact
    32761 ttaccactaa aggcagagtt atcaccggtt aagtaagtcg tgccattgag cgcattgata
    32821 tagcccaggc cctcgctgcc actgacaata tccgcggaaa tagtcgcgtc tttatccgtg
    32881 tggtttaagg tgattttctt atcggatgtc gaagcccaca caaactcaat tttttcggca
    32941 tcaataatcc ctgccgcctt cgcagcactt ccttcctgtg cacctaacgc taactcagag
    33001 ttggtgctta aactaatttt cggtgcggat attttacctc catctgatat gactaatgtt
    33061 gtatcagaac gttggtcgta ataacccagg ctaatatgtt cacgagcatt aacttcacca
    33121 ccgttattaa ccgacaggaa ctgattaccg ccatatccgc cgtaaacgcc cataaagaag
    33181 gtccctgcat ttacctgact accttcgccg cttacattcc cctgcgtata agtcagacga
    33241 ctggtgccct taacatgaat tttgccgcca ttggagacat taaaatagcc ttgatcatgt
    33301 ctcatgcctg agttttactc atacaaatca tcagcatata actcgctgcc agcattggag
    33361 acattcagga accccgtacc tttcagcaaa aagttaagtg ggccattgat agttaccttt
    33421 cctccggtaa tatctaaccc accaccagaa gcgagagtca gacgcggacg ggaagtatct
    33481 gtcgcattga ttaccagatt ggcattgtca gtcaccgtaa tacggtaatc gatgctctgg
    33541 ttctctttgc caaaatcaat agttttatcc tggtttacga tgacatcatc ggcgaatacc
    33601 agagatgaat ataaacttat cgcgctaatt aaaacagccc gggacttttt tccttttact
    33661 ctgctcccca gctccgagca gacgctccaa cagttacggg acctgttcca ctttagccga
    33721 tatattttat tcatcacaat ctccagcaat acatccaaat aaaaccaaga ttgcataaac
    33781 agcaacgcat taaatgccat tctggttact gttcatcgca tataaaaatt caaaaatttt
    33841 gcatagcatt ggcaaccaca tatctgagta tgtagttcag agggtaataa aatagcaagt
    33901 tattacttaa aacttatttt aagttaacac tcttaacttt atcattacat tcctccca
//
LOCUS       DAZKIV010000031        33841 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZKIV010000031 DAZKIV010000000
VERSION     DAZKIV010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33841
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.031"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(157..1044)
                     /gene="rarD"
                     /locus_tag="ACHOT6_001548"
     CDS             complement(157..1044)
                     /gene="rarD"
                     /locus_tag="ACHOT6_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFP9946788.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1096..1563)
                     /gene="yigI"
                     /locus_tag="ACHOT6_001549"
     CDS             complement(1096..1563)
                     /gene="yigI"
                     /locus_tag="ACHOT6_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFP9946789.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1728..2597
                     /gene="pldA"
                     /locus_tag="ACHOT6_001550"
     CDS             1728..2597
                     /gene="pldA"
                     /locus_tag="ACHOT6_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFP9946790.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2730..4559
                     /gene="recQ"
                     /locus_tag="ACHOT6_001551"
     CDS             2730..4559
                     /gene="recQ"
                     /locus_tag="ACHOT6_001551"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFP9946791.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTVCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4623..5243
                     /gene="rhtC"
                     /locus_tag="ACHOT6_001552"
     CDS             4623..5243
                     /gene="rhtC"
                     /locus_tag="ACHOT6_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFP9946792.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5305..5925)
                     /gene="rhtB"
                     /locus_tag="ACHOT6_001553"
     CDS             complement(5305..5925)
                     /gene="rhtB"
                     /locus_tag="ACHOT6_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFP9946793.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6036..7058
                     /gene="pldB"
                     /locus_tag="ACHOT6_001554"
     CDS             6036..7058
                     /gene="pldB"
                     /locus_tag="ACHOT6_001554"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFP9946794.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7066..7866
                     /gene="yigL"
                     /locus_tag="ACHOT6_001555"
     CDS             7066..7866
                     /gene="yigL"
                     /locus_tag="ACHOT6_001555"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFP9946795.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7942..8841
                     /gene="bioP"
                     /locus_tag="ACHOT6_001556"
     CDS             7942..8841
                     /gene="bioP"
                     /locus_tag="ACHOT6_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFP9946796.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8729..9682)
                     /gene="metR"
                     /locus_tag="ACHOT6_001557"
     CDS             complement(8729..9682)
                     /gene="metR"
                     /locus_tag="ACHOT6_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFP9946797.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9918..12179
                     /gene="metE"
                     /locus_tag="ACHOT6_001558"
     CDS             9918..12179
                     /gene="metE"
                     /locus_tag="ACHOT6_001558"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFP9946798.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALVLGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12301..12423)
                     /locus_tag="ACHOT6_001559"
     CDS             complement(12301..12423)
                     /locus_tag="ACHOT6_001559"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946799.1"
                     /translation="MRKNDSVISVSERAQIAKWLPVMEAIRLLAKCALFMPDAA"
     gene            complement(12464..12709)
                     /locus_tag="ACHOT6_001560"
     CDS             complement(12464..12709)
                     /locus_tag="ACHOT6_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFP9946800.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12699..13184)
                     /locus_tag="ACHOT6_001561"
     CDS             complement(12699..13184)
                     /locus_tag="ACHOT6_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin"
                     /protein_id="HFP9946801.1"
                     /translation="MAGASIVAGAVLGRMRLPDLESLEHFGARGAVSGRPFNPDLAGG
                     PIENLTTDGVTINREGIAIVEKHIARFGHDPVNEVMINRLKDIEKGKIPPEQVDLNFY
                     THECREYQRYCNLGWETGQPDGDAGYALWNHTHTATLEDYKLKGELNDLYHQDALDYD
                     N"
     gene            complement(13164..13825)
                     /gene="tssD"
                     /locus_tag="ACHOT6_001562"
                     /pseudo
     CDS             complement(13164..13825)
                     /gene="tssD"
                     /locus_tag="ACHOT6_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
     gene            complement(14218..15030)
                     /gene="ysgA"
                     /locus_tag="ACHOT6_001563"
     CDS             complement(14218..15030)
                     /gene="ysgA"
                     /locus_tag="ACHOT6_001563"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFP9946802.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            15292..16053
                     /gene="udp"
                     /locus_tag="ACHOT6_001564"
     CDS             15292..16053
                     /gene="udp"
                     /locus_tag="ACHOT6_001564"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFP9946803.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            16194..17621
                     /gene="rmuC"
                     /locus_tag="ACHOT6_001565"
     CDS             16194..17621
                     /gene="rmuC"
                     /locus_tag="ACHOT6_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFP9946804.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFSLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            17716..18471
                     /gene="ubiE"
                     /locus_tag="ACHOT6_001566"
     CDS             17716..18471
                     /gene="ubiE"
                     /locus_tag="ACHOT6_001566"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFP9946805.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18485..19090
                     /gene="ubiJ"
                     /locus_tag="ACHOT6_001567"
     CDS             18485..19090
                     /gene="ubiJ"
                     /locus_tag="ACHOT6_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFP9946806.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19087..20727
                     /gene="ubiB"
                     /locus_tag="ACHOT6_001568"
     CDS             19087..20727
                     /gene="ubiB"
                     /locus_tag="ACHOT6_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFP9946807.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            20806..21075
                     /gene="tatA"
                     /locus_tag="ACHOT6_001569"
     CDS             20806..21075
                     /gene="tatA"
                     /locus_tag="ACHOT6_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFP9946808.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21079..21594
                     /gene="tatB"
                     /locus_tag="ACHOT6_001570"
     CDS             21079..21594
                     /gene="tatB"
                     /locus_tag="ACHOT6_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFP9946809.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            21597..22373
                     /gene="tatC"
                     /locus_tag="ACHOT6_001571"
     CDS             21597..22373
                     /gene="tatC"
                     /locus_tag="ACHOT6_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFP9946810.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22415..23197
                     /gene="tatD"
                     /locus_tag="ACHOT6_001572"
     CDS             22415..23197
                     /gene="tatD"
                     /locus_tag="ACHOT6_001572"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFP9946811.1"
                     /translation="MFDIGGNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRVAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23194..23682)
                     /gene="rfaH"
                     /locus_tag="ACHOT6_001573"
     CDS             complement(23194..23682)
                     /gene="rfaH"
                     /locus_tag="ACHOT6_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFP9946812.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            23849..25342
                     /gene="ubiD"
                     /locus_tag="ACHOT6_001574"
     CDS             23849..25342
                     /gene="ubiD"
                     /locus_tag="ACHOT6_001574"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFP9946813.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDIVAHIDAIWDELAIFNNGKSA"
     gene            25388..26089
                     /gene="fre"
                     /locus_tag="ACHOT6_001575"
     CDS             25388..26089
                     /gene="fre"
                     /locus_tag="ACHOT6_001575"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFP9946814.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26281..27444)
                     /gene="fadA"
                     /locus_tag="ACHOT6_001576"
     CDS             complement(26281..27444)
                     /gene="fadA"
                     /locus_tag="ACHOT6_001576"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFP9946815.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27454..29643)
                     /gene="fadB"
                     /locus_tag="ACHOT6_001577"
     CDS             complement(27454..29643)
                     /gene="fadB"
                     /locus_tag="ACHOT6_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFP9946816.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVNQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            29833..31164
                     /gene="pepQ"
                     /locus_tag="ACHOT6_001578"
     CDS             29833..31164
                     /gene="pepQ"
                     /locus_tag="ACHOT6_001578"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFP9946817.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31164..31778
                     /gene="yigZ"
                     /locus_tag="ACHOT6_001579"
     CDS             31164..31778
                     /gene="yigZ"
                     /locus_tag="ACHOT6_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFP9946818.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            31817..33268
                     /gene="trkH"
                     /locus_tag="ACHOT6_001580"
     CDS             31817..33268
                     /gene="trkH"
                     /locus_tag="ACHOT6_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFP9946819.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33280..33825
                     /gene="hemG"
                     /locus_tag="ACHOT6_001581"
     CDS             33280..33825
                     /gene="hemG"
                     /locus_tag="ACHOT6_001581"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFP9946820.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHTAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7958 a         8511 c         9093 g         8279 t
ORIGIN      
        1 gtagatatac gatgtctcta ggatggagta agtactgggg agttttgggg gtatttcctg
       61 taacaaatat tgttcttttt cttatcttaa tttttttcaa aaaaagataa ataagtcagt
      121 gggtaggctc tttaaacggc ctacccatgt taattcttaa ctttttctac gctgcgtata
      181 aatcgcatcc atcacaaaaa ttgccagcgc cacccaaata aaggcgaaag tcaccatctt
      241 atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca gcgtcgggcc
      301 aatgtactgg aaaaagccta acgttgagag acgcaagcgc gtggcagcgg cggtaaaaca
      361 caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca gcgacatcgg
      421 gttttgcccc atatggctgg ttgagctgtc ggcaatagca aacaggtaaa ttgccgccac
      481 gggcagcagc cacatggttt cgattaacat gccggtttgc gcttcaacgg caatcttctt
      541 gcgtaccaga ccgtagaagg caaaactaaa tgccagtccc agcgcgataa taggtagcga
      601 accaaaagtc cacagctgga ctaacacgcc acatatcgcc agaatcaccg ccagccattg
      661 catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca ccagcgggtt
      721 aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc agataaacag
      781 tagccagttg ccaccaatca gcacggcaga gactgccagc ataaaaattt tctgtggcgt
      841 ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca ccaccataaa
      901 gaaaaacgac cagatcacgc gatgcgtcag gatttcatcg gcgggcacgt agtaaatcaa
      961 cttgaagtac gctggcgcta taccccaaat aaaataagcg gcaagagcga gtaatacgcc
     1021 ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa cagcagttta
     1081 cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa tataaagctg
     1141 ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac gcaacaggct
     1201 actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc gaagatcaat
     1261 ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt cgtggcgggt
     1321 taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg caatgacccc
     1381 gccgtgcaaa atgctttgcg cccagttgcc caccatcatt ggctgatttt taaaggccag
     1441 ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt taaatggcat
     1501 gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca gtacggcaga
     1561 catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc tttgttgttg
     1621 gtttctactt taggaaggga taactaacgg ctatggagtt aagtatgtaa aatagcccgc
     1681 agaaaaatat tcaccttatc aataattcgt tacggagaac acgaccgatg cggactctgc
     1741 agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa gaggcaacgg
     1801 tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat atgctgcagg
     1861 agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatt tacacccaaa
     1921 ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat gcgcgtaagg
     1981 atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt ttaggcccga
     2041 actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc aatagcgaag
     2101 agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt tttgccaccg
     2161 attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac cacgactcta
     2221 acgggcgttc cgacccgacc tcccgcagct ggaaccgcct ttatactcgc ctgatggcag
     2281 aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat actgacgata
     2341 acccggatat caccaaatat atgggttact accagcttaa aatcggctat cacctcggtg
     2401 atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc ggcgcggagt
     2461 taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt tacagcggct
     2521 atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgtg ggggttatgc
     2581 taaacgattt gttttgatga acggttgagt ggttggcaaa tctggaaccc agcatccagg
     2641 attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga aaatagcgcc
     2701 tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg aatctggagt
     2761 ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc cccggccagg
     2821 aagaaattat cgacactgtg ctttccggtc gcgattgcct ggtcgtcatg cccaccggtg
     2881 gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt accgtggttg
     2941 tttcaccgct gatttcgttg atgaaagatc aggtggatca actgcaagcc aacggcgtgg
     3001 cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg atgacagtct
     3061 gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg ctggataact
     3121 ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa gcgcactgta
     3181 tctcccaatg gggccacgat ttccgcccgg aatatgccgc gctcggtcag ttgcgccagc
     3241 ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc acgcgccagg
     3301 atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt tttgaccgtc
     3361 cgaatattcg ctacatgctg atggagaagt tcaaaccgct cgatcagttg atgcgctacg
     3421 tgcaggaaca gcgcggtaag tcaggcatta tctactgcaa cagccgcgcg aaagtagaag
     3481 acaccgctgc gcgcctgcaa agcaagggaa ttagcgcggc ggcctatcat gccgggctgg
     3541 aaaataatgt tcgcgccgat gtgcaggaaa aattccagcg cgatgacctg caaattgtgg
     3601 tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc gtggtccact
     3661 ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggacgcgcc gggcgtgatg
     3721 gcctgcccgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg ctgcgccgtt
     3781 gtctggaaga gaagccgcag gggcagttgc aggatatcga acgtcataaa ctcaatgcga
     3841 tgggcgcttt tgccgaagcg caaacttgcc gtcgtctggt actgctgaac tactttggcg
     3901 aagggcggca ggagccgtgc ggaaactgcg atatctgcct cgatccgccg aaacagtacg
     3961 acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat cagcggtttg
     4021 ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc cgcgactatg
     4081 gtcatgacaa actgaaagtc tatggcatgg gccgtgataa aagccatgaa cattgggtga
     4141 gcgtgatccg ccagctgatt cacctcggcc tggtgacgca aaatattgca cagcattctg
     4201 ccctccaact gacagaggct gcgcgtccgg tgctgcgcgg cgaatcctct ttgcaacttg
     4261 ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc ggcggcaact
     4321 atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc gatgaaagta
     4381 atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct gaacagatgc
     4441 cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag ctggaacgct
     4501 ttggcaaacc gtttatggcg ctgattcgtg cgcatgttga tggcgatgac gaagagtagt
     4561 cagcagcata aaaaagtgcc aggatgaaga ctccgtaaac gtttcccccg cgagtcaaat
     4621 gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt atgagccccg
     4681 gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa gaagcgatga
     4741 tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg ttgcttggcc
     4801 tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggtg ggcggtggcc
     4861 tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa gaggctgtat
     4921 ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg aaaggcttac
     4981 tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc tcattgtttg
     5041 tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt attgtcgaaa
     5101 cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg cgccgtggtt
     5161 atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc ggatttggca
     5221 ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg gataaggcgt
     5281 ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca gtgctcccac
     5341 cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg gtcctttaat
     5401 ccatagagca atccgttgag caagggtggc gtaaccgatc atcacaataa tatcgaccac
     5461 aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg gcatgatgaa
     5521 ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat tcacaaaaac
     5581 tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa ggtcaattgc
     5641 accagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc ctgcccactt
     5701 caacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca cgccaaccag
     5761 cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg cgccgcgata
     5821 accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg acaggcttaa
     5881 aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga actcccggtg
     5941 tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca cgctaaaaaa
     6001 aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa aaagactggg
     6061 aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat ttctggcgtc
     6121 agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt gtccgttttc
     6181 gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag agctacgtaa
     6241 aatatgcgga actggcctat gacctgttcc atttggggtt tgatgtctta atcatcgacc
     6301 atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg catgttaatc
     6361 gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt cagcccggtc
     6421 cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc acattatttc
     6481 tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg tttgggatcg
     6541 tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa gcgcatccac
     6601 gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg tttgctatca
     6661 acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat gctgatgacc
     6721 caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt ctggctggcg
     6781 aacaggtgtt agccggtgcg ggtgatgacg ccacgccaac gcttctctta caagctgaag
     6841 aggaacgcgt ggtggataac cgcatgcatg accgtttttg tgaactccgc accgccgcgg
     6901 gccatcctgt cgaaggagga cgaccgttgg taattaaagg tgcttaccat gagatccttt
     6961 ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat tgttgatttt ttcaacaggc
     7021 ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta ccaggttgtt
     7081 gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc ttacgccaaa
     7141 gaaaccctga agcttctcac cgcgcgcggc atcaactttg tgtttgcgac cggtcgtcac
     7201 cacgttgatg tggggcaaat tcgcgataat ctggagatca agtcgtacat gatcacctcc
     7261 aacggcgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa cttggatcgc
     7321 gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat tactaacgtt
     7381 tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg cttttttaaa
     7441 gaagcggtgt tcaaatatgc gctgtatgag cctggattac tggagccgga aggcgtcagc
     7501 aaagtgttct tcacctgcga ttcccatgaa caactgctgc cgctggagca ggcgattaac
     7561 gctcgttggg gcgatcgcgt caacgttagt ttctctacct taacctgtct ggaagtgatg
     7621 gcgggcggcg tttcaaaagg ccatgcgctg gaagcggtgg cgaagaaact gggctacagc
     7681 ctgaaggatt gtattgcgtt tggtgacggg atgaacgatg ccgaaatgct gtcgatggcg
     7741 gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagaccttca tcccgagctg
     7801 gaagtgattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa actctattta
     7861 tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa tggatactcg
     7921 ttaatcaaag agttttccat tgtggcgcta cttatcatca ccacgattct gtgggccttc
     7981 tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt tgcggtgctg
     8041 gtgcgcgttg gcctggcggc actcgttttt ctgccgtttc tgcgtacccg tggcaatagc
     8101 ctgaaaacag tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt gatgtatatg
     8161 ctgagtttcc gcgcttatct ctacctgacg gtttccgaac tgctgctgtt caccgtactg
     8221 acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct gcgctgggga
     8281 tatgctttta gtgccttgtt ggcggtgatt ggtgccggga ttattcgcta tgatcaggtc
     8341 accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac ttttgccatc
     8401 ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagca taacgccttt
     8461 gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt gctgggaaat
     8521 gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct tggcgtggtg
     8581 gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga cgccggaacg
     8641 ctgggcatta tgaataatat gcacgttccg gcagggctgc tggttaacct ggctatctgg
     8701 caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct ggcctcactg
     8761 tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga tcgcaggcgt
     8821 gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg ccatcgcgca
     8881 cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc accagaccct
     8941 ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc cgcgcggcaa
     9001 ccatctgaat caacaataag gtgttatcga cgcttttcag tgacgggctg acgcctgccg
     9061 gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa attaatagcg
     9121 tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga tggtcaggtg
     9181 ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca ctgcgcggca
     9241 ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc tgcgggtcaa
     9301 acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg aaattttcta
     9361 acgcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc agacgcgtct
     9421 gctgcggttc attgcaggct tgtagtgcct ggctgatttg cggcagcacc tggtttgcca
     9481 gttgcaacag gatttctccc tgcggtgtaa agcgaagcgg ctggctttta cgcacaaata
     9541 gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg gattgcgtct
     9601 gatgcaacgt cgccgcagcg gctgcgagag agccgcagtt ccgcaacgct tgtagcgttt
     9661 tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat ttgcgcttga
     9721 ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta aaatccttcg
     9781 tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta cttcagtaag
     9841 ctcccgggga tgaataaact tgccgccttc cctaaattca aaatccatag gatttacata
     9901 taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct cgcgttggcc
     9961 tgcgtcgcga gctgaaaaaa gcgcaagaga gttattgggc agggaactcc acgcgtgaag
    10021 aactgctggc ggtagggcgt gaattgcgtg ctcgtcactg ggatcaacaa aagcaagcgg
    10081 gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg accaccagtt
    10141 tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta gatatcgaca
    10201 ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg gcagcggaaa
    10261 tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt aaaggccaac
    10321 agttcaaact gacctggacg cagctgctgg aggaagtgga cgaggcgctg gtgctgggcc
    10381 acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg aaagtgaaag
    10441 gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat cagcaagtgc
    10501 tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc gcgctggtac
    10561 tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg ctccagggac
    10621 aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc gacacgatta
    10681 ctgcgctgcc tgttcagggt ctgcatgttg acctcgtaca tggtaaagat gacgttgctg
    10741 aactgcacaa gcgcctgcct tctgactggt tgctgtctgc gggtctgatc aatggtcgta
    10801 acgtctggcg cgccgatctt accgagaaat atgcgcaaat taaggacatt gtcggcaaac
    10861 gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac ctgagcgtgg
    10921 aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa aaatgccatg
    10981 aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg gcagagtgga
    11041 gcgccccgat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggca gtagaaaagc
    11101 gtctggcggc gatcaccgct caggacagtc agcgtgcgaa tgtctatgaa gtgcgtgctg
    11161 aagcccagcg tgcgcgtttt aaactgccag catggccgac caccacgatt ggttctttcc
    11221 cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat ctcgacgcca
    11281 acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag caggaacgtt
    11341 tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg gaatactttg
    11401 gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc tacggttccc
    11461 gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg attaccgtgg
    11521 agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg ctgacggggc
    11581 cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa accatcgcca
    11641 aacagattgc gctggcgctg cgtgatgagg tggccgatct ggaagcggca gggattggta
    11701 tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt agcgactggg
    11761 acgcctatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg gcgaaagatg
    11821 acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg gattccattg
    11881 cggcgctgga tgcggatgtg attaccatcg aaacttcgcg ttctgacatg gagttgctgg
    11941 agtcgttcga agagtttgat tatccgaacg aaatcggtcc aggcgtctat gacattcact
    12001 cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg gcaaaacgca
    12061 ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc ggctggccag
    12121 aaacccgcgc agcactggcg aatatggtgc aggcggcgca gaatttgcgt cggggatgat
    12181 gaatattaac gagtcactgt aaattgcagt acgatgaaag aggcaaaact gcctgatacc
    12241 aggcctacat aatttatgca atttattgaa tttttgggtt ttgttggccg gacaaggcgt
    12301 tcacgccgca tccggcatga acaacgcgca ctttgccaat aatctgatgg cttccatcac
    12361 cggaagccat tttgctatct gcgcccgttc agatacagaa ataaccgaat catttttacg
    12421 caaaatataa tcctgagcga tttgttcagt aatcaacgct acgttattta ttctcacttt
    12481 tcacgaaatt gtatcccttt tcagtaacgt ggtagtgagg catagactta tgtccgctat
    12541 cctcaataat aaccaaaccc tcgttagcta atttgttaac aatgttaaca acattaccga
    12601 atcctggaat ttgccttatg gatagtgtgc gatcgagatt catccagttc cagtttatat
    12661 ctttctcagc cataactttc agtacgttaa tttcagtttc agttatcata gtcaagtgca
    12721 tcctggtggt ataagtcatt aagttctccc ttaagtttat aatcttctag agtagcagta
    12781 tgcgtatgat tccagagtgc atagcctgcg tcaccatcag gttgtcctgt ttcccaacca
    12841 agattacagt aacgctgata ttcccgacac tcatgagtat agaagttaag atctacttgt
    12901 tctgggggga tttttccttt ctcaatgtct ttcagtctat tgatcatcac ttcatttaca
    12961 gggtcatgac caaatctggc tatgtgtttt tcaacaatag cgataccttc cctgttgatt
    13021 gttacgccat ctgtcgtcag attttctatt ggaccaccgg caaggtcggg attaaatggt
    13081 cttcctgata ctgctcctct ggctccgaaa tgctcaagtg attccaaatc agggagtctc
    13141 attctcccca gaactgctcc agcaactatt gaagcaccag cgatgagtaa ccctttgtta
    13201 tccataaatt gatcgtaacc agcaggtgca tctcccccca acttgtctgc tgtttccctg
    13261 aatccttcga tattcccatt gtatatgccg acagcagcaa gcagtctgcc aaccgcttta
    13321 ctattaagtg ttttgaaacc attaccagct ttagtttttg gccgtggtat aaacagacgg
    13381 ttgtagttat cgggaaatgc ccaataacta aattcagtat tagcaatcag atgcctggag
    13441 agtatatatt aataactgac agtgatggct tctgtatcca gattgttctc agtaaacagg
    13501 tgattgattg ctgataagag cgcgccgcgg agttgaatgt tgtaatattt ctcccatcga
    13561 ccaaacctat tgatccgata gaagtcaaac tccatgaaca actgctcgtt attgttaata
    13621 gcgttaataa gaatttacag aaactaaggc catgcaattg tgaccctttg cctgcattcg
    13681 taatactatt agagagcgag aacgcgaaga tttcatcttc atggccaatc tgccagcgat
    13741 tacctattga tgcgctcgtt ccacaaagag aggaaataca tccttgttgt tctccggtta
    13801 tggtcaagta aataatatca ctcatcctta aaaaaccttt tgttaattct tttgtgaaag
    13861 agaaatagta ttgttgtttt tttaccttta tttacgccta aaagtgatga actaaaatgt
    13921 gaaaaacaaa atgtttcact ttaatatctt tttatcagga aggtctgaga tgatttataa
    13981 ttaatgtgaa tgtgttgata ataattaatg tttgtttgtg aatgggagta gatggtaaat
    14041 ctctaaggtg tcttccattg aagagactca tttatgacta tgacttgccg actttcttaa
    14101 ggtcaaatag cggagtcgta atctccaatc aattcatact gattattttt tgggggacag
    14161 agatggcagc aaaacgcact cgttcactca ccatctcaaa tatagactaa gaggaaacta
    14221 actccgcttc ccaccatact gcttaaacca ttccagcata cgctgccagc catctttcgc
    14281 agattcggca tgatagctcg ggcgataatc agcgttgaat gcatgcccgg cgtccgggta
    14341 cacgataatc tctgctttcg cattagcagc ccgcagcgcc tggcgcatgg tttcaacgct
    14401 ctcctgcgga atgctgttat cctgaccacc atataagccg agaaccggcg cgttaagatc
    14461 ggttgcgata tcaacaggtt gtttcggtga attcagcgac ttgtcgcccg tcagtttgcc
    14521 gtaccacgcc actgcggctt ttagctgtgg attatgcgcg gcatacagcc aggtgatacg
    14581 tccaccccag cagaatccgg tgatcattaa acgatgaaca tcgccgccgt tgcgggaagc
    14641 ccaactggcg acatgatcga gatcggccag cacctgcgag tcaggcactt ttgctaccag
    14701 accgctaagc aacgtgggga tatcggcaaa atcattcgga tcgccttcgc ggaagtaaag
    14761 ttcaggtgcg atagccagat acccctccag cgccagacgg cgacaaatgt cgcggatatg
    14821 ttcatgcacg ccaaaaattt cctgcactac aatgaccact ggcagtgggc catcgctttg
    14881 ctttggtctg gcatggtaag caggcatgtt atccccttgt gaagggatag aggtgaagcc
    14941 cgccacaatc gcgtcgtccg gggtctgaac gatggtcgaa gcgagaggcg atgcagcagg
    15001 tgcaaatcca gattgttgtg ttgttgccat ggtattctcc gtacccttat aaaaatgttg
    15061 cgcaatgtta actatagtca gcatgcaaca aatcacattg cctgaatcgg ctcatctttt
    15121 atgcagtcct gcagaatgaa gagtgattta tgtgatttgc atcacttttg gtgggtaaat
    15181 ttatgcaacg catttgcgtc atggtgatga gtatcacgaa aaaatgttaa acccttcggt
    15241 aaagtgtctt tttgcttctt ctgactaaac cgattcacag aggagttgta tatgtccaag
    15301 tctgatgttt ttcatctcgg cctcactaaa aacgatttac aaggggctac gcttgccatc
    15361 gtccctggcg acccggatcg tgtggaaaag atcgccgcgc tgatggataa gccggttaag
    15421 ctggcatctc accgcgaatt caccacctgg cgtgcagagc tggatggtaa acctgttatc
    15481 gtctgctcta ccggtatcgg cggcccgtct acctctattg ctgttgaaga gctggcacag
    15541 ctgggcattc gcaccttcct gcgtatcggt acaacgggcg ctattcagcc gcatattaat
    15601 gtgggtgatg tcctggttac cacggcgtct gtccgtctgg atggcgcgag cctgcacttc
    15661 gcaccgctgg aattcccggc tgtcgctgat ttcgaatgta cgactgcgct ggttgaagct
    15721 gcgaaatcca ttggcgcgac aactcacgtt ggcgtgacag cttcttctga taccttctac
    15781 ccaggccagg aacgttacga tacttactct ggtcgcgtag ttcgtcactt taaaggttct
    15841 atggaagagt ggcaggcgat gggcgtaatg aactatgaaa tggaatctgc aaccctgctg
    15901 accatgtgtg caagtcaggg cctgcgtgcc ggtatggtag cgggtgttat cgttaaccgc
    15961 acccagcaag agatcccgaa tgctgagacg atgaaacaaa ctgaaagcca agcggtgaaa
    16021 atcgtggtgg aagcggcgcg tcgtctgctg taattctctt ctcctgtctg aaggccgacg
    16081 cgttcggcct tttgtatttt tgcgtagcgc ctcgcaggaa atgcctttcc aactggacgt
    16141 ttgtacagca caattctatt ttgtgcgggt aagttgttgc gtcaggaggc gttgtggatt
    16201 tctcaatcat ggtttacgca gttattgcgt tggtgggtgt ggcaattggc tggctgtttg
    16261 ccagttatca acatgcgcag caaaaagccg agcaattagc tgaacgtgaa gagatggtcg
    16321 cggagttaag cgcggcaaaa caacaaatta cccaaagcga gcactggcgt gcagagtgcg
    16381 agttactcaa taacgaagtg cgcagcctgc aaagtattaa cacctctctg gaggccgatc
    16441 tgcgtgaagt aaccacgcgg atggaagccg cacagcaaca tgctgacgat aaaattcgcc
    16501 agatgattaa cagcgagcag cgcctcagtg agcagtttga aaacctcgcc aaccgtattt
    16561 ttgagcacag caatcgccgg gttgatgagc aaaaccgtca gagtctgaac agcctgttgt
    16621 cgccgctacg tgaacaactg gacggtttcc gccgtcaggt tcaggacagc ttcggtaaag
    16681 aagcacaaga acgccatacc ctgacccacg aaattcgcaa tctccagcaa ctcaacgcgc
    16741 aaatggccca ggaagcgatc aacctgacgc gcgcgctgaa aggcgacaat aaaacccagg
    16801 gcaactgggg cgaggtagta ttgacgcggg tgctggaggc ttccggtctg cgtgaagggt
    16861 atgaatatga aacccaggtc agcatcgaaa atgacgcccg ctcgcggatg cagccggatg
    16921 tcatcgtgcg gctgccgcag ggaaaagatg tggtgatcga cgccaaaatg acgctggtcg
    16981 cctatgaacg ctattttaac gccgaagacg actacacccg cgaaagcgcg ctacaggaac
    17041 atatcgcgtc ggtgcgtaac catatccgtt tgctgggacg caaagattat caacagctgc
    17101 cggggctgcg aactctggat tacgtgctga tgtttattcc cgttgaaccc gctttttcac
    17161 tggcgcttga ccgccagccg gagctgatca ccgaagcgtt gaaaaacaat atcatgctgg
    17221 ttagcccgac tacgctgctg gtggcgttgc gtactattgc caacctgtgg cgttatgagc
    17281 atcaaagccg caacgcccag caaatcgccg atcgtgccag caagctgtac gacaagatgc
    17341 gtttgttcat cgatgacatg tccgcgattg gtcaaagtct cgacaaagcg caggataact
    17401 accgccaggc aatgaaaaaa ctctcttcag gtcgcggaaa tgtgctggcg caggcagaag
    17461 cgtttcgcgg tttaggagta gaaattaaac gcgagattaa tccggatttg gctgaacagg
    17521 cggtgagcca ggatgaagag tatcgacttc ggtcggttcc ggagcagccg aatgatgaag
    17581 cttatcaacg cgatgatgaa tataatcagc agtcgcgcta gcccattagg ggtagttaag
    17641 ccgggtagaa atctagggca tcgacgccca atctgttaca cttctggaac aattttttga
    17701 tgagcaggca ttgagatggt ggataagtca caagaaacga cgcactttgg ttttcagacc
    17761 gtcgcgaagg aacaaaaagc ggatatggtc gcccacgttt tccattccgt ggcatcaaaa
    17821 tacgatgtca tgaatgattt gatgtcattt ggtattcatc gtttgtggaa gcgattcacg
    17881 attgattgca gcggcgtacg ccgtgggcag accgtgctgg atctggctgg tggcaccggc
    17941 gacctgacag cgaaattctc ccgcctggtc ggagaaactg gcaaagtggt ccttgctgat
    18001 atcaatgaat ccatgctcaa aatgggacgc gagaagctgc gtaatatcgg tgtgattggc
    18061 aacgttgagt atgttcaggc gaacgctgag gcgctgccgt tcccggataa cacctttgat
    18121 tgcatcacca tttcgtttgg tctgcgtaac gtcaccgaca aagataaagc actgcgttca
    18181 atgtatcgcg tgctgaaacc cggcggccgc ctgctggtgc ttgagttctc gaagccaatt
    18241 atcgagccgc tgagcaaagc ctatgatgca tactccttcc atgtgctgcc gcgtattggc
    18301 tcactggtcg cgaacgacgc cgacagctac cgttatctgg cagaatccat ccgtatgcat
    18361 cccgatcagg ataccctgaa agccatgatg caggatgccg gattcgaaag tgtcgactac
    18421 tacaatctga cggcaggggt tgtggcgctg catcgtggtt ataagttctg acaggagacc
    18481 ggaaatgcct tttaaacctt tagtgacggc aggaattgaa agtctgctca acaccttcct
    18541 gtatcgctca cccgcgctga aaacagcccg ctcgcgtctg ctgggtaaag tattgcgcgt
    18601 ggaggtaaaa ggcttttcga cgtcattgat tctggtgttc agcgaacgcc aggttgatgt
    18661 actgggcgaa tgggcaggcg atgctgactg caccgttatc gcctacgcca gtgtgttgcc
    18721 gaaacttcgc gatcgccagc agcttaccgc actgattcgc agtggtgagc tggaagtgca
    18781 gggcgatatt caggtggtgc aaaacttcgt tgcgctggca gatctggcag agttcgaccc
    18841 tgcggaactg ctggcccctt ataccggtga tatcgccgct gaaggaatca gcaaagccat
    18901 gcgcggaggc gcaaagttcc tgcatcacgg cattaagcgc cagcaacgtt atgtggcgga
    18961 agccattact gaagagtggc gtatggcacc cggtccgctt gaagtggcct ggtttgcgga
    19021 agagacggct gccgtcgagc gtgctgttga tgccctgacc aaacggctgg aaaaactgga
    19081 ggctaaatga cgccaggtga agtacggcgc ctatatttca tcattcgcac ttttttaagc
    19141 tacggacttg atgaactgat ccccaaaatg cgtatcaccc tgccgctacg gctatggcga
    19201 tactcattat tctggatgcc aaatcggcat aaagacaaac ttttaggtga gcgactacga
    19261 ctggccctgc aagaactggg gccggtttgg atcaagttcg ggcaaatgtt atcaacccgc
    19321 cgcgatcttt ttccaccgca tattgccgat cagctggcgt tattgcagga caaagttgct
    19381 ccgtttgatg gcaagctggc gaagcagcag attgaagctg caatgggcgg cttgccggta
    19441 gaagcgtggt ttgacgattt tgaaatcaag ccgctggctt ctgcttctat cgcccaggtt
    19501 cataccgcgc gattgaaatc gaatggtaaa gaggtggtga ttaaagtcat ccgcccggat
    19561 attttgccgg tcattaaagc ggatctgaaa cttatctacc gtctggctcg ctgggtgccg
    19621 cgtttgctgc cggatggtcg ccgtctgcgc ccaaccgaag tggtgcgcga gtacgaaaag
    19681 accttgattg atgaactgaa tttgctgcgg gaatctgcca acgccattca gcttcggcgc
    19741 aattttgaag acagcccgat gctctacatc ccggaagttt accctgacta ttgtagtgaa
    19801 gggatgatgg tgatggagcg catttacggc attccggtgt ctgatgttgc ggcgctggag
    19861 aaaaacggca ctaacatgaa attgctggcg gaacgcggcg tgcaggtgtt cttcactcag
    19921 gtctttcgcg acagcttttt ccatgccgat atgcaccctg gcaacatctt cgtaagctat
    19981 gaacacccgg aaaacccgaa atatatcggc attgattgcg ggattgttgg ctcgctaaac
    20041 aaagaagata aacgctatct ggcagaaaac tttatcgcct tctttaatcg cgactatcgc
    20101 aaagtggcag agctacacgt cgattcaggc tgggtgccac cagataccaa cgttgaagag
    20161 ttcgaatttg ccattcgtac ggtctgtgaa cctatctttg agaaaccgct ggccgaaatt
    20221 tcgtttggac atgtactgtt aaatctgttt aatacggcgc gtcgcttcaa tatggaagtg
    20281 cagccgcaac tggtgttact ccagaaaacc ctgctctatg tcgaaggggt aggacgccag
    20341 ctttatccgc agctcgattt atggaaaaca gcgaagcctt tcctggagtc gtggattaaa
    20401 gatcaggtcg gtattcctgc gctggtgaga gcatttaaag aaaaagcgcc gttctgggtc
    20461 gaaaaaatgc cagaactgcc tgaattggtt tacgacagtt tgcgccaggg caagtattta
    20521 cagcacagtg ttgataagat tgcccgcgag cttcagtcaa atcatgtacg tcagggacaa
    20581 tcgcgttatt ttctcggaat tggcgctacg ttagtattaa gtggcacatt cttgttggtc
    20641 agccgacctg agtgggggct gatgcccggc tggttaatgg caggtggtct gatcgcctgg
    20701 tttgtcggtt ggcgcaaaac acgctgattt tttcatcgct caaggcgggc cgtgtaacgt
    20761 ataatgcggc tttgtttaat catcatctac cacagaggaa catgtatggg tggtatcagt
    20821 atttggcagt tattgattat tgccgtcatc gttgtactgc tttttggcac caaaaagctc
    20881 ggctccatcg gttccgatct tggtgcgtcg atcaaaggct ttaaaaaagc aatgagcgat
    20941 gatgaaccaa agcaggataa aaccagtcag gatgctgatt ttactgcgaa aactatcgcc
    21001 gataagcagg cggatacgaa tcaggaacag gctaaaacag aagacgcgaa gcgccacgat
    21061 aaagagcagg tgtaatccgt gtttgatatc ggttttagcg aactgctatt ggtgttcatc
    21121 atcggcctcg tcgttctggg gccgcaacga ctgcctgtgg cggtaaaaac ggtagcgggc
    21181 tggattcgcg cgttgcgttc actggcgaca acggtgcaga acgaactgac ccaggagtta
    21241 aaactccagg agtttcagga cagtctgaaa aaggttgaaa aggcgagcct cactaacctg
    21301 acgcccgaac tgaaagcgtc gatggatgaa ctacgccagg ccgcggagtc gatgaagcgt
    21361 tcctacgttg caaacgatcc tgaaaaggcg agcgatgaag cgcacaccat ccataacccg
    21421 gtggtgaaag acaatgaagc tgcgcatgaa ggcgtaacgc ctgctgctgc acaaacgcag
    21481 gccagttcgc cggaacagaa gccagaaacc acgccagagc cggtggtaaa acctgctgcg
    21541 gacgctgaac cgaaaaccgc tgcaccttcc ccttcgtcga gtgataaacc gtaaacatgt
    21601 ctgtagaaga tactcaaccg cttatcacgc atctgattga gctgcgtaag cgtctgctga
    21661 actgcattat cgcggtgatc gtgatattcc tgtgtctggt ctatttcgcc aatgacatct
    21721 atcacctggt atccgcgcca ttgatcaagc agttgccgca aggttcaacg atgatcgcca
    21781 ccgacgtggc ctcgccgttc tttacgccga tcaagctgac ctttatggtg tcgctgattc
    21841 tgtcagcgcc ggtgattctc tatcaggtgt gggcatttat cgccccagcg ctgtataagc
    21901 atgaacgtcg cctggtggtg ccgctgctgg tttccagctc tctgcttttt tatatcggca
    21961 tggcattcgc ctactttgtg gtctttccgc tggcatttgg cttccttgcc aataccgcgc
    22021 cggaaggggt gcaggtatcc accgacatcg ccagctattt aagcttcgtt atggcgctgt
    22081 ttatggcgtt tggtgtctcc tttgaagtgc cggtagcaat tgtgctgctg tgctggatgg
    22141 ggattacctc gccagaagac ttacgcaaaa aacgcccgta tgtgctggtt ggtgcattcg
    22201 ttgtcgggat gttgctgacg ccgccggatg tcttctcgca aacgctgttg gcgatcccga
    22261 tgtactgtct gtttgaaatc ggtgtcttct tctcacgctt ttacgttggt aaagggcgaa
    22321 atcgggaaga ggaaaacgac gctgaagcag aaagcgaaaa aactgaagaa taaattcaac
    22381 cgcccgtcag ggcggttgtc atatggagta caggatgttt gatatcggcg gtaatttgac
    22441 cagttcgcaa tttgcgaaag accgtgatga tgttgtagcg cgcgcttttg acgcgggagt
    22501 taatgggcta ctcatcaccg gcactaacct gcgtgaaagc cagcaggcgc aaaagctggc
    22561 gcgtcagtat tcgtcctgtt ggtcaacggc gggcgtacat cctcacgaca gcagccagtg
    22621 gcaagctgcg actgaagaag cgattattga gctggccgcg cagccagaag tggtggcgat
    22681 tggtgaatgt ggtctcgact ttaaccgcaa cttttcgacg ccggaagagc aggaacgcgc
    22741 ttttgttgct cagctacgca ttgccgcaga attaaacatg ccggtattta tgcactgtcg
    22801 cgatgcccac gagcggttta tgacattgct ggagccgtgg ctggataaac tgcctggtgc
    22861 ggttcttcat tgctttaccg gcacacgcga agagatgcag gcgtgcgtgg cgcgtggaat
    22921 ttatatcggc attaccggtt gggtttgcga tgaacgacgc ggactggagc tgcgggaact
    22981 tttgccgttg attccggcgg aaaaattact gatcgaaact gatgcgccgt atctgctccc
    23041 tcgcgatctc acgccaaagc catcatcccg gcgcaacgag ccagcccatc tgccccatat
    23101 tttgcaacgt gttgcgcact ggcgtggaga agatgccgca tggctggctg caaccacgga
    23161 tgccaatgtc aaaacactgt ttgggattgc gttttagagt ttgcggaact cggtgttctt
    23221 cacactgtgc ttaatctctt tattaataag attaagcaat agcatggagc gagcctcacc
    23281 atcgggttcg gtgaaaatgg cctgaaagcc ttcgaacgcg ccttcggtaa taatcacctt
    23341 atcacccgga taaggggttg ccggatcgac aatgtctttc ggtttatata ccgatagctg
    23401 atgaatcacc gccgatggga ctatcgctgg cgacgcgcca aagcgcacga agtggctgac
    23461 accgcgggtc gcgttgatag tcgtggtatg aatcacttct ggatcaaatt cgacaaacag
    23521 atagttggga aacaatggct cactgactgc agtacgtttt ccacgcacga ttttttccag
    23581 ggtgatcatc ggtgccaggc aattcacagc ctgtctttcg aggtgttcct gggcacgttg
    23641 aagttgcccg cgcttgcagt acagtaaata ccaggattgc ataatgactc ttatccgttt
    23701 aatcggggcg caaggatagc aaaagcttta cgctaagtta attatattcc ccggtttgcg
    23761 ttataccgtc agagttcacg ctaatttaac aaatttacag catcgcaaag atgaacgccg
    23821 tataatgggc gtagattaag aggctacaat ggacgccatg aaatataacg atttacgcga
    23881 cttcctgaca ctgcttgaac agcagggtga gctaaaacgt atcacgctcc cggtggatcc
    23941 gcatctggaa atcactgaaa ttgctgaccg cactttgcgt gccggtgggc ctgcgctgtt
    24001 gttcgaaaac cctaaaggct actcaatgcc ggtgctgtgc aacctgttcg gtacgccaaa
    24061 gcgcgtggcg atgggcatgg ggcaggaaga tgtttcggcg ctgcgtgaag ttggtaaatt
    24121 attggcgttt ctgaaagagc cggagccgcc aaaaggtttc cgcgacctgt ttgataaact
    24181 gccgcagttt aagcaagtat tgaacatgcc gacaaagcgg ctgcgtggtg cgccctgcca
    24241 acaaaaaatc gtctctggcg atgacgtcga tctcaatcgc attcccatta tgacctgctg
    24301 gccggaagat gccgcaccgc tgattacctg ggggctgaca gtgacgcgcg gcccgcataa
    24361 agagcggcag aatctgggca tttatcgcca gcagctgatt ggtaaaaaca aactgattat
    24421 gcgctggctg tcgcatcgcg gcggcgcgct ggattatcag gagtggtgtg cggcgcatcc
    24481 gggcgaacgt ttcccggttt ctgtggcgct gggtgccgat cccgccacga ttctcggtgc
    24541 agtcaccccc gttccggata cactttcaga gtatgcgttt gccggattgc tgcgcggtac
    24601 caagaccgaa gtggtgaagt gtatctccaa tgaccttgaa gtgcccgcca gtgcggagat
    24661 tgtgttggaa gggtatatcg aacaaggcga aactgcgccg gaagggccgt atggcgacca
    24721 caccggttac tataacgaag tcgatagttt cccggtattt accgtgacgc atattaccca
    24781 gcgtgaagat gcgatttacc attccaccta taccgggcgt ccgccagatg agcccgcggt
    24841 actgggcgtg gcgttgaacg aagtgtttgt accgattctg caaaaacagt tcccggaaat
    24901 tgtcgatttt tacctgccgc cggaaggctg ttcttatcgt ctggcggtag tgacgatcaa
    24961 aaaacagtac gccggacacg cgaagcgcgt catgatgggc gtctggtcgt tcttacgcca
    25021 gtttatgtac actaaatttg tgatcgtttg cgatgatgac gtcaacgcac gcgactggaa
    25081 cgatgtgatt tgggcgatta ccacccgtat ggacccggcg cgggatactg ttctggtaga
    25141 aaatacgcct attgattatc tggattttgc ctcgcctgtc tccgggctgg gttcaaaaat
    25201 ggggctggat gccacgaata aatggccggg ggaaacccag cgtgaatggg gacgtcccat
    25261 caaaaaagat ccagatattg tcgcgcatat tgacgccatc tgggatgaac tggctatttt
    25321 taacaacggt aaaagcgcct gatgcgcgtt tgttttgccc tatttatcga tccgacagag
    25381 aaagcgcatg acaaccttaa gctgtaaagt gacctcggta gaagctatca cggataccgt
    25441 atatcgtgtc cgcatcgtgc cagacgcggc cttttctttt cgtgctggtc agtatttgat
    25501 ggtagtgatg gatgagcgcg acaaacgtcc gttctcaatg gcctcgacgc cggatgaaaa
    25561 agggtttatc gagctgcata ttggcgcttc tgaaatcaac ctttacgcga aagcagtcat
    25621 ggaccgcatc ctcaaagatc atcaaatcgt ggtcgatatt ccccacggag aagcgtggct
    25681 gcgcgatgat gaagagcgtc cgatgatttt gattgcgggc ggcaccgggt tctcttatgc
    25741 ccgctcgatt ttgctgacag cgttggcgcg taacccaaac cgtgatatca ccatttactg
    25801 gggcgggcgt gaagagcagc atctgtatga tctctgcgag cttgaggcgc tttcgttaaa
    25861 gcaccctggt ctgcaagtgg tgccggtggt tgaacaaccg gaagcaggct ggcgtgggcg
    25921 tactggtacc gtgttaacgg cggtattgca ggatcatggt acgctggcag agcatgatat
    25981 ctatattgcc ggacgttttg agatggcgaa aattgcccgt gacctgtttt gcagtgagcg
    26041 taatgcgcgg gaagatcgcc tgtttggcga tgcgtttgca tttatctgag atataaaaaa
    26101 acccgcccct gacaggcggg aagaacggca actaaaactt tattcagtgg catttagatc
    26161 tatgtcgcat ccggcaattg gtgcacgacg cctgatgcga cgctggcgcg tcttatcagg
    26221 cctacattgg tgccggatcg gtagaccgga taaggcgttc acgccgcatc cggcaagtgg
    26281 ttaaacccgc tcaaacaccg tcgcaatacc ctgacccaga ccgatacaca tcgtcgccag
    26341 accaaactga acgtctttgc gttccatcag attcagcagc gtggtgctga tacgcgcacc
    26401 ggaacagccc agcggatgac ccagcgcgat cgcaccacca ttgaggttga tcttctcgtc
    26461 aatctgctcc attagcccga gatctttaat gcacggcagg atctgcgcgg caaaggcttc
    26521 gttcatctca aacacgccga tatcgctggc agaaagcccc gcttttttca gcgccagctt
    26581 cgaggccgga accgggccgt aacccataat cgatgggtca caaccaacga ccgccatcga
    26641 acgcacacga gcgcgcggct taagacctaa ttcatgggcg cggctttcac tcatcaccag
    26701 catggcagct gcgccatcgg aaagtgcaga agatgtgccc gccgttaccg taccgtttac
    26761 tggatcaaac gccggacgca gcgtggcgag ggcttccacg gtggtttccg ggcgaatcac
    26821 ttcgtcgtaa ttaaactgct tcaggacgcc gtcggcatcg tgaccaccag ttgggatgat
    26881 ttcattttta aatgcggccg actgcgtggc ggcccaggcg cgggcgtgtg accgcgcggc
    26941 aaaggcatcc tgcatttcac ggctgatacc gtgcatacgc gccagcattt ctgccgttaa
    27001 gcccatcatg cccgccgctt tggcgacatt gcggctcagg ccggggtgaa aatcgacgcc
    27061 gtgactcatc ggcacatggc ccatatgctc cacgccgcca accagacatg cctgcgcatc
    27121 gccagtcatg atcattcgtg ctgcgtcatg cagtgcctgc atggatgaac cacacaagcg
    27181 attgacggta accgccggga cagagtgcgg cacttctgcc agcagcgccg cgttacgggc
    27241 gatattaaaa ccctgctcca gcgtctgctg cacacaaccc cagtaaatat cgtcgagggc
    27301 cgccgcttcc agcgccgggt tacgcgccag caggctacgc attaaatgag cggagagatc
    27361 ttctgcacgc acgttacgaa aagcaccgcc cttcgaacgg cccatcgggg tgcgaattgc
    27421 atcgacaatg acaacctgtt ccattgtgac tccttaagcc gttttcaggt cgccaaccgg
    27481 acgggctggc tcaaccggag gatagtacgg ttcgttatga cgcgctttat tacgcagacc
    27541 ttccggcact tcatacagcg ggccgaggtg ctgatattgc tgtgccatat cgaggtattt
    27601 tgcgctaccg agggtgtcca gccagcggaa cgcgccgccg tggaacggag ggaagcccag
    27661 gccgtagacc agcgccatat ccgcttccgc cggagtggcg ataatgcctt cctccagaca
    27721 gcgcaccact tcgttgacca tcgggatcat catgcgggcg ataatctctt cttcgctgaa
    27781 atcgcgcttc ggctggttca cttctgccag caggtcttca acggcggcgt cttcttcttt
    27841 cttcggctta cctttgctgt cttctttata acgccagaaa ccgaggccgt tcttctgacc
    27901 aaagcggttg gcatcaaaca gcgcgtcgat ggcatcgcgg taatctttct gcatccgctg
    27961 cgggaagcct gctgccatga cagcctgagc gtgatgcgcg gtatcaatgc ccacgacatc
    28021 cagcagatat gccggaccca taggccagcc aaactgtttt tccatcactt tgtcgatctt
    28081 gcggaaatcc gcgccgtcgc gcagcagctg gctgaaaccg gcgaaatacg ggaacagcac
    28141 gcggttaaca aagaagccgg ggcagtcgtt aaccacaatc ggcgtcttgc ccatcttgct
    28201 cgcccaggcg acaactttcg cgatggtttc gtcggagctt ttctcgccgc gaataatttc
    28261 taccaacggc atccggtgta ccgggttaaa aaagtgcatt ccgcagaagt tttccgggcg
    28321 ttccagcgcg ttggccagtt cgctgatagg aatggttgaa gtgttagacg ccagcacggt
    28381 atccgggcgt actttttgtt cggtttctgc cagtacggct tttttcactt tcgggttttc
    28441 gacaaccgct tctaccacaa catccacgcg gtcaaacccg gcgtagtcga gcgttgggtg
    28501 gattgtggag atcacgccag ccagtttcag accatcgatc ttgccgcgct caagctgctt
    28561 gttcagcagt ttcgcggctt cggtcatgcc gagggttaac gacttgtcgt tgatatcttt
    28621 catgacaacc ggcacgcctt tccacgcaga ctggtaagcg atgccgccgc ccataatgcc
    28681 tgcacccagc accgcggcct gtttcggggt ttcaacgtct ttggtgagtt tcttcgcttt
    28741 gccttttaca tattgatcgt taaggaaaat gccgaccagt gcgcgggctt cgttggtatg
    28801 cgccagcggg acaaaacttt tgttttccag gtttaaggct tcttcacgac caaaacgggc
    28861 cgcagcttca atggttttta ctgcggtgat gggggccgga taatgtttcc ccgctgtttg
    28921 tgcgaccatc cctttagcga tggtgaagct catggtggct tcaatcttgc tcagttttag
    28981 tggttccagc ttcggctgac gttttgcttt ccagtcgagg tcgccgttaa tggcctggcg
    29041 taaaaccgcc tttgcgcctt caaccagttt ttctgctttg actacgccat ccaccagacc
    29101 gattttcagc gcctgatccg cgccgacatc tttaccggcg gcaatgattt ccagcgcact
    29161 gtcagcgccc agcatacgtg gcatacgtac agaaccgcca aagcccggca tgatgcccag
    29221 tttggtttcc ggcagaccga tgcgcagatc cggcgtcgcc agacgataat cggtcgccag
    29281 cacgcattcg cagccaccgc ccagcgcata gccgttgacg gcagcaatgg tcggcaccgg
    29341 cagatcttcc aggcgattaa acacgctatt ggcaaagtgc agccactgac ttaactgttc
    29401 ttcaggaacg aggaacaggg acaaaaattc ggtgatatca gcaccgacga taaaggctgc
    29461 tttgttcgaa cgcagcagca gcccttttag atctgattgc tgttccagca cgccgatggc
    29521 ctcgccgagg ctggcgacgg tcgcagtgtc gagtttatta actgaacctg gggcatcaaa
    29581 taccagttcg gcaatgccat cttccagcca gtcaaggtac agggtgtcgc ctttgtaaag
    29641 catgtcagtc tcctgaatcc gcaaggtgat ctggtcgtac cagatgagtc gaagtgtgta
    29701 ttttgtgtta aaaatatgca aataaaagat taaagaaatg ccaatctgat cacgctcggc
    29761 agaaatcacg ctctggatga acgatgtgct aagatgcgga gacttaaggt caaaaaaaca
    29821 gaagggtaaa aaatggaatc actggcctcg ctctataaaa atcatatagc taccttacaa
    29881 gaacggactc gcgatgcgct ggcgcgcttc aagctggatg cattacttat tcactccggc
    29941 gagctgttca acgtttttct cgacgatcat ccctatccgt ttaaagtgaa cccgcaattc
    30001 aaagcgtggg tgccggtaac tcaggtgcca aactgctggt tgctggtgga tggcgtgaac
    30061 aagccgaaac tgtggttcta tctgccggtt gattactggc acaacgtcga accgctgccg
    30121 acctccttct ggactgaaga tgtggaagtg atcgcactgc cgaaagccga tggcattggt
    30181 agcctgctgc ccgctgcgcg cggcaatatc ggttatatcg gtccggtgcc ggaacgtgcg
    30241 ctgcaactgg gtattgaggc cagcaacatc aacccgaaag gggtgatcga ctacctgcat
    30301 tactatcgct ccttcaaaac cgagtacgaa ctggcctgta tgcgtgaagc gcagaaaatg
    30361 gcggtcaacg gtcatcgcgc ggcagaagaa gcgttccgtt ctggcatgag cgagttcgat
    30421 atcaacattg cctatctgac cgcgaccggt catcgtgata ccgacgtacc ttacagcaac
    30481 attgtggcgc ttaacgaaca cgctgcggtg ctgcattaca ccaaactgga ccaccaggca
    30541 ccggaagaga tgcgcagctt cctgctggat gccggggccg aatataacgg ctatgcggct
    30601 gacctgaccc gtacctggtc ggcaaaaagt gacaacgact acgcacagct ggtgaaggac
    30661 gttaatgatg aacaactggc gctgatcgct accatgaaag caggcgtcag ctatgtggat
    30721 taccacatcc agttccatca gcgcatcgcc aaactgctgc gtaaacatca aatcatcacc
    30781 gatatgagtg aagaagcgat ggtcgaaaac gatctcaccg gaccgtttat gccgcacggt
    30841 atcggtcatc cgctgggcct gcaggtgcat gacgtagccg gttttatgca ggatgatagc
    30901 ggtacacacc tcgcggcacc ggcaaaatat ccgtacctgc gctgcacccg tattctccag
    30961 ccgggtatgg tgttaaccat cgaaccgggt atctacttca ttgaatcgct actggcaccg
    31021 tggcgtgaag ggcagttcag caagcacttc aactggcaga aaattgaagc actgaaaccg
    31081 ttcggcggca ttcgtatcga agacaacgtg gtgatccacg aaaataacgt ggaaaacatg
    31141 acccgggatc tgaaactggc gtgatggaaa gctggttaat tcctgcggca ccggtcacgg
    31201 tcgttgaaga gatcaaaaag agccgcttca taacgatgtt ggcgcatacc gatggcgttg
    31261 aggcggcgaa agcgtttgtt gaatcggtgc gggcagaaca ccccgatgcc cgccaccatt
    31321 gcgtggcgtg ggtggcgggt gcgccggatg attctcaaca gctgggtttc tctgacgacg
    31381 gggagccggc gggaacggca ggtaaaccga tgctcgccca gctaatgggc agcggcgtcg
    31441 gggaaattac cgctgtggta gtgcgctact acggcggcat attgcttggc accggtgggt
    31501 tagtgaaagc gtatggcggc ggcgtgaatc aggcgctgcg ccagctaacg acccaacgca
    31561 agacgccatt aaccgaatat actttgcaat gtgaatatag tcagttaacc ggcattgaag
    31621 cgttgctggg gcagtgtgac ggcaaaatta tcaacagtga ttatcaggca ttcgttctgc
    31681 tgcgggtggc gcttccggcg gcgaaagtgg ctgaattttc cgcaaagctg gcggatttta
    31741 gccgtggttc attgcaattg ttagcgattg aagaataatc cccacttcgt tttgcagaac
    31801 taaggaagcg gcagagatgc attttcgcgc cattacccga atcgttggac tactggtcat
    31861 cttattttca gggaccatga ttatccctgg gctggtagca ctcatctacc gggatggagc
    31921 gggccgcgct tttacccaga ccttttttgt cgccctcgcc attggttcta tgctgtggtg
    31981 gccgaaccgt aaagagaagg gcgaactgaa atcccgtgag gggtttctga tagtggtgct
    32041 gttctggacc gtgctgggta gtgtcggtgc gctccctttt atcttctcgg aaagcccgaa
    32101 cctcacgatt accgatgcgt tttttgaatc tttctctggc ctgaccacca ccggggccac
    32161 tacgctggtg gggctggatt cgctccctca tgccatcctc ttttatcgcc agatgctgca
    32221 atggtttggc gggatgggga tcatcgtgtt agcggttgcg atactgccta tcctcggcgt
    32281 gggtgggatg cagctctatc gcgcagaaat gcccggcccg ctgaaagata acaaaatgcg
    32341 cccgcgaatt gcggaaacgg cgaaaaccct gtggttgatt tatgtcttgc tgaccgtcgc
    32401 ctgtgcgctg gcgttgtggt ttgccggaat ggatgccttt gatgccatcg gccatagctt
    32461 tgcgactatc gctattggcg gcttctcgac acatgatgcc agtatcggtt atttcgacag
    32521 cccgactatt aacactatca ttgctatctt cctgctgatc tccggctgta actacggtct
    32581 gcacttttca ctgttaagtg ggcgtagtct gaaggtttat tggcgcgatc cggaatttcg
    32641 catgtttatc ggcgtacagt ttacgctggt ggttatttgt acactcgtac tgtggtttca
    32701 taatgtctac agttcggcgc tgatgacaat taaccaggcg tttttccagg tggtatcgat
    32761 ggcgacaacc gccgggttta cgactgacag cattgcccgc tggccgctct ttttgccggt
    32821 actgctttta tgttcagctt ttatcggcgg ttgcgccggg tcaacgggcg gtggcctgaa
    32881 agtgatccgc atcctgctgc tgtttaagca ggggaaccgt gagctgaaac gactggtgca
    32941 tccgaacgcc gtctatagca ttaagctggg gaatcgcgca ctgccggaac gtatcctcga
    33001 agccgtttgg ggatttttct ccgcctatgc attggtgttt attgtcagta tgctggcgat
    33061 tatcgccacg ggcgtggatg acttttctgc ctttgcctca gttgttgcga cattgaataa
    33121 cctggggcca gggcttggcg tggttgctga taactttacc agtatgaacc cggtggctaa
    33181 atggatcctg attgccaaca tgctgtttgg tcgtctcgag gtctttacat tgctggtgct
    33241 ctttaccccg actttctggc gtgaatgatg gagtaatacg tgaaaacatt aattcttttc
    33301 tcaacaaggg acggacaaac gcgcgagatt gcctcctacc tggcttcgga actgaaagaa
    33361 ctggggatcc agacggatgt cgccaatgtg caccgcattg aagaaccaca atgggaaaat
    33421 tatgaccgtg tggtcattgg tgcttctatt cgctatggtc actaccacac tgcgttccag
    33481 gaatttgtca aaaaacatgc gacgcgactg aattcgatgc cgagcgcctt ttattccgta
    33541 aacctggtgg cgcgcaaacc ggagaagcgt actccacaga ccaacagcta cgcgcggaag
    33601 tttctgatga actcgcaatg gcgtcccgat cgctgcgcgg tcattgccgg ggcgctgcgt
    33661 tacccacgtt atcgctggta cgaccgtttt atgatcaagc tgattatgaa gatgtcaggc
    33721 ggtgaaacgg atacgcgcaa agaagttgtc tataccgatt gggagcaggt ggcgaatttc
    33781 gcccgagaaa tcgcccattt aaccgacaaa ccgacgctga aataagcata aagaataaaa
    33841 a
//
LOCUS       DAZKIV010000032        32717 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZKIV010000032 DAZKIV010000000
VERSION     DAZKIV010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.032"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHOT6_001582"
     CDS             complement(<1..281)
                     /gene="tccP2"
                     /locus_tag="ACHOT6_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
                     /protein_id="HFP9946821.1"
                     /translation="MINSINSFFSSIPRSISSVTRNSSFTASQHKSTPNTVKTSSPLS
                     PSNSPASATTIFKVKNSYTESGLQRSTSYTQSSIEKNALHRPLPDVAKRL"
     gene            complement(727..1131)
                     /locus_tag="ACHOT6_001583"
                     /pseudo
     CDS             complement(727..1131)
                     /locus_tag="ACHOT6_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
     gene            complement(1214..1301)
                     /locus_tag="ACHOT6_001584"
     tRNA            complement(1214..1301)
                     /locus_tag="ACHOT6_001584"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1265..1267),aa:Ser,seq:tga)
     gene            1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHOT6_001585"
     CDS             1728..2846
                     /gene="hyaA"
                     /locus_tag="ACHOT6_001585"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFP9946822.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHOT6_001586"
     CDS             2843..4636
                     /gene="hyaB"
                     /locus_tag="ACHOT6_001586"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFP9946823.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHOT6_001587"
     CDS             4655..5362
                     /gene="hyaC"
                     /locus_tag="ACHOT6_001587"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFP9946824.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHOT6_001588"
     CDS             5359..5946
                     /gene="hyaD"
                     /locus_tag="ACHOT6_001588"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFP9946825.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNCDCLSMENYEGVRLRQYRMTQEEQG"
     gene            5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHOT6_001589"
     CDS             5943..6341
                     /gene="hyaE"
                     /locus_tag="ACHOT6_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFP9946826.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGS
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHOT6_001590"
     CDS             6338..7195
                     /gene="hyaF"
                     /locus_tag="ACHOT6_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFP9946827.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            7329..8873
                     /gene="appC"
                     /locus_tag="ACHOT6_001591"
     CDS             7329..8873
                     /gene="appC"
                     /locus_tag="ACHOT6_001591"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFP9946828.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFALKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            8885..10021
                     /gene="appB"
                     /locus_tag="ACHOT6_001592"
     CDS             8885..10021
                     /gene="appB"
                     /locus_tag="ACHOT6_001592"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9946829.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHOT6_001593"
     CDS             10034..10126
                     /gene="cbdX"
                     /locus_tag="ACHOT6_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFP9946830.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            10206..11504
                     /gene="appA"
                     /locus_tag="ACHOT6_001594"
     CDS             10206..11504
                     /gene="appA"
                     /locus_tag="ACHOT6_001594"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFP9946831.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(11619..13799)
                     /gene="etk"
                     /locus_tag="ACHOT6_001595"
     CDS             complement(11619..13799)
                     /gene="etk"
                     /locus_tag="ACHOT6_001595"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFP9946832.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(13819..14265)
                     /gene="etp"
                     /locus_tag="ACHOT6_001596"
                     /gene_synonym="yccY"
     CDS             complement(13819..14265)
                     /gene="etp"
                     /locus_tag="ACHOT6_001596"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFP9946833.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(14253..15392)
                     /gene="gfcE"
                     /locus_tag="ACHOT6_001597"
     CDS             complement(14253..15392)
                     /gene="gfcE"
                     /locus_tag="ACHOT6_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFP9946834.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(15438..17534)
                     /gene="gfcD"
                     /locus_tag="ACHOT6_001598"
     CDS             complement(15438..17534)
                     /gene="gfcD"
                     /locus_tag="ACHOT6_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9946835.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRNWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(17534..18280)
                     /gene="gfcC"
                     /locus_tag="ACHOT6_001599"
     CDS             complement(17534..18280)
                     /gene="gfcC"
                     /locus_tag="ACHOT6_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9946836.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(18277..18921)
                     /gene="gfcB"
                     /locus_tag="ACHOT6_001600"
     CDS             complement(18277..18921)
                     /gene="gfcB"
                     /locus_tag="ACHOT6_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFP9946837.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(19028..19333)
                     /gene="gfcA"
                     /locus_tag="ACHOT6_001601"
     CDS             complement(19028..19333)
                     /gene="gfcA"
                     /locus_tag="ACHOT6_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HFP9946838.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(19775..19987)
                     /gene="cspH"
                     /locus_tag="ACHOT6_001602"
     CDS             complement(19775..19987)
                     /gene="cspH"
                     /locus_tag="ACHOT6_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFP9946839.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            20273..20485
                     /gene="cspG"
                     /locus_tag="ACHOT6_001603"
     CDS             20273..20485
                     /gene="cspG"
                     /locus_tag="ACHOT6_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFP9946840.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            20496..20684
                     /gene="ymcF"
                     /locus_tag="ACHOT6_001604"
     CDS             20496..20684
                     /gene="ymcF"
                     /locus_tag="ACHOT6_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9946841.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHVPLDFTK"
     gene            20659..20889
                     /gene="ymcE"
                     /locus_tag="ACHOT6_001605"
     CDS             20659..20889
                     /gene="ymcE"
                     /locus_tag="ACHOT6_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFP9946842.1"
                     /translation="MCRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            20879..21052
                     /gene="gnsA"
                     /locus_tag="ACHOT6_001606"
     CDS             20879..21052
                     /gene="gnsA"
                     /locus_tag="ACHOT6_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9946843.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(21101..22174)
                     /gene="yccM"
                     /locus_tag="ACHOT6_001607"
     CDS             complement(21101..22174)
                     /gene="yccM"
                     /locus_tag="ACHOT6_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9946844.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIGCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(22246..24990)
                     /gene="torS"
                     /locus_tag="ACHOT6_001608"
     CDS             complement(22246..24990)
                     /gene="torS"
                     /locus_tag="ACHOT6_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFP9946845.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVADLEAWLNKKDLNAI
                     "
     gene            25073..26101
                     /gene="torT"
                     /locus_tag="ACHOT6_001609"
     CDS             25073..26101
                     /gene="torT"
                     /locus_tag="ACHOT6_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFP9946846.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(26074..26766)
                     /gene="torR"
                     /locus_tag="ACHOT6_001610"
     CDS             complement(26074..26766)
                     /gene="torR"
                     /locus_tag="ACHOT6_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFP9946847.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            26896..28068
                     /gene="torC"
                     /locus_tag="ACHOT6_001611"
     CDS             26896..28068
                     /gene="torC"
                     /locus_tag="ACHOT6_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFP9946848.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKDASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            28068..30623
                     /gene="torA"
                     /locus_tag="ACHOT6_001612"
     CDS             28068..30623
                     /gene="torA"
                     /locus_tag="ACHOT6_001612"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFP9946849.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     EAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYEQLKAKVAAGEVEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAHTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAESIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKSWPR"
     gene            30611..31210
                     /gene="torD"
                     /locus_tag="ACHOT6_001613"
     CDS             30611..31210
                     /gene="torD"
                     /locus_tag="ACHOT6_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFP9946850.1"
                     /translation="MATLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LRQWLPEFAARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(31303..31608)
                     /gene="cbpM"
                     /locus_tag="ACHOT6_001614"
     CDS             complement(31303..31608)
                     /gene="cbpM"
                     /locus_tag="ACHOT6_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFP9946851.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(31608..32528)
                     /gene="cbpA"
                     /locus_tag="ACHOT6_001615"
     CDS             complement(31608..32528)
                     /gene="cbpA"
                     /locus_tag="ACHOT6_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFP9946852.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFQHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         7755 a         8316 c         8596 g         8050 t
ORIGIN      
        1 aggcgtttgg ccacatcagg tagaggccga tgtaaagcgt ttttttctat acttgactga
       61 gtataagatg tggaacgttg aagtcctgat tccgtataag aattttttac cttaaaaata
      121 gttgttgcac tggcaggact gttgctggga gaaagaggtg agctggtttt taccgtgtta
      181 ggtgtgcttt tgtgctgtga ggcagtgaag ctactgttac gcgtaacact tgatatactg
      241 cgaggaatgc tggaaaaaaa agaattaatg ctatttatca tatagataac cttataagaa
      301 ctttatgtga tagtctaatt tatccgtttg catgaaatct accttttgtg aaattctcgt
      361 caatatttta ctcaaaatga tcggctgtag gaataaacta tttgatatta ttgcgttttt
      421 agagttgttt tcaatcagct atagataggg cttggcgcca gaatggagaa tatttaacga
      481 gaaatagaag ctttgtatca gacgggcttc cctgaataga gaagttaaat aatcaggatg
      541 atgctaatga atatcaggaa caaggtgagt gtcttcatgt tcactgaaac agggtgtcac
      601 aaaaaatatg gctataagat gatcaatgtg atttgaaata tttataggtt tacagggggc
      661 tgggcatgca tatcttactg caatgcagaa tatgtgctgc ttggcgcagc tctttattga
      721 gaatgttcaa tcttttgttt cagtttcgtg aaggatacgg acggtttctt gtgtaatatt
      781 atacatcgcc tgtttatctt tactgcatac tcctgcaagt gttaatgcta ctccagcata
      841 aatttctgga taactatgtg taaatatatc cagtgagttt acatgtttct ctttcattcc
      901 tgcatcacaa cgaaccttat ttatacctgt cgccattgat tggggagggc tagagccttc
      961 tgcacgaaaa tagtgatgtg acagatctga gatagctttg tatccttctg cctggttcag
     1021 gtctggagca gtccatgttt tacaaaaata ctcggcagcg taacgtgttg caagagcggc
     1081 atcagacgag gaacctgagg ttccgctcat gaccgataat ctcatccgaa taaaaatata
     1141 ccaattggta taccaagttt ataccaagag ccaaaaaaca aaggggctac catttggtaa
     1201 ccccttgatt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     1261 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     1321 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     1381 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     1441 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     1501 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa aagtttcact
     1561 acgctttatt aacaatactt tctggcgacg tgtgccagtg cagaaggatg agctttcgtt
     1621 ttcagcatct cacgtgaagc gatggtttgc cttgctacag ggacgtcgct tgccgaccat
     1681 aagcgcccgg tgtcctgccg gtgttgctag gagaagagac gtgcgatatg aataacgagg
     1741 aaacatttta ccaggccatg cggcgtcagg gcgttacccg gcgcagcttt ctcaaatatt
     1801 gtagtctggc tgccacgtcg ctgggattag gcgcgggaat ggcaccaaag attgcctggg
     1861 cgctggagaa caaaccccgc attccggtgg tatggatcca cggtctggaa tgcacctgct
     1921 gtaccgaatc ttttatccgc tccgctcacc cactggcgaa ggacgtcatc ctttccctga
     1981 tttccctcga ttatgacgat actttgatgg ctgccgccgg aacccaggcg gaagaagtct
     2041 tcgaagacat catcacgcaa tacaatggca aatatatcct cgcagtagaa ggtaatccgc
     2101 cgctgggcga gcaggggatg ttctgtatca gcagcggtcg accgtttatt gagaaactca
     2161 aacgtgccgc tgccggagcc agcgcgatta tcgcctgggg aacctgcgcg tcctggggct
     2221 gcgtgcaggc cgcgcggccc aatccgacgc aggcaacgcc tatcgacaaa gtcatcaccg
     2281 acaaacccat tatcaaagta cctggctgcc cgccgatccc ggatgtgatg agcgccatca
     2341 ttacttacat ggtgaccttt gatcgcttgc cagatgtcga cagaatgggc cgtccgctga
     2401 tgttctatgg tcagcgaatc cacgataaat gctatcgccg cgcccacttc gacgccggag
     2461 agttcgtcca gagttgggat gatgacgctg cccgcaaagg ttactgcctg tacaaaatgg
     2521 gctgcaaagg gcctaccacc tataacgcct gttcctccac acgctggaat gatggcgttt
     2581 cttttccaat ccagtctggt cacggctgcc tgggctgtgc ggaaaatggt ttctgggatc
     2641 gcggttcgtt ctacagccgc gtggtcgata ttccgcaaat gggtactcat tccactgccg
     2701 ataccgtcgg tttaaccgcg cttggcgtgg tggcagcggc tgttggtgtg cacgcagtcg
     2761 ccagcgccgt tgaccagcgc agacgtcata accagcaacc tacagaaacc gaacatcagc
     2821 caggcaatga ggataaacag gcatgagcac tcagtacgaa actcagggat acaccatcaa
     2881 taatgccgga cgccgcctgg tggtcgaccc gattacgcgc atcgaaggcc acatgcgctg
     2941 cgaagtgaat attaacgatc agaatgtgat caccaatgcc gtctcctgcg gcaccatgtt
     3001 tcgcgggctg gagatcattc tgcaagggcg cgacccgcgc gatgcgtggg cgttcgttga
     3061 acgtatctgc ggcgtctgta ctggcgtaca cgccctggct tcggtttacg ccatcgaaga
     3121 tgctatcggt attaaagtgc cggacaacgc caatatcatc cgcaacatta tgctggcaac
     3181 gctctggtgc cacgatcatc tggtgcactt ctatcagctt gccgggatgg actggatcga
     3241 tgtgttagat gcgctgaaag ccgacccgcg gaaaacctcc gaactggcgc aaagtctctc
     3301 ctcttggccg aaatcatccc cgggctattt cttcgacgta caaaaccgcc tgaaaaaatt
     3361 cgttgaaggc gggcagttgg ggatcttccg caatggctac tgggggcacc cgcagtacaa
     3421 gttgccgcca gaagccaacc tgatgggctt tgcccactac ctcgaagctc tcgatttcca
     3481 gcgtgaaatt gtgaaaatcc acgcggtctt tggcggtaaa aacccacatc caaactggat
     3541 tgtcggcggg atgccttgcg ccattaacat tgacgaaagc ggcgcggtcg gggcggtcaa
     3601 tatggaacgc ctgaacctgg tgcagtcgat cattacccgc acggcggatt tcattaacaa
     3661 cgtgatgatc cccgacgcct tagccatcgg tcagttcaac aaaccgtgga gcgaaatcgg
     3721 cacgggtctt tctgataaat gcgttctcag ctacggcgca ttcccggata ttgccaacga
     3781 ctttggcgag aaaagtctgc tgatgcctgg cggcgcggtg attaacggcg acttcaacaa
     3841 tgtgctgcca gtggatttgg ttgatccgca gcaggtgcag gagtttgtcg accacgcctg
     3901 gtatcgatat cccaacgatc aggtcgggcg tcatccgttc gatggcatca ccgacccgtg
     3961 gtacaacccc ggcgatgtca aaggcagcga taccaacatt cagcagctga atgaacagga
     4021 acgctactcg tggatcaaag cgccacgctg gcgcggtaac gcgatggaag tggggccgct
     4081 ggcgcgcacg ttaatcgctt atcacaaagg cgatgctgcg accgttgagt cggtcgatcg
     4141 catgatgtcg gcgttgaacc tgccgctttc cggtatccag tcaacgttag gccgcatttt
     4201 gtgccgcgcg cacgaagcgc agtgggccgc aggtaagttg cagtatttct tcgacaagct
     4261 gatgaccaac ctgaaaaacg gcaatctcgc cactgcttcc acggaaaaat gggaacctgc
     4321 aacctggccg acagagtgcc gtggtgtcgg ttttaccgaa gcgccgcgcg gggcgttagg
     4381 ccactgggcc gccattcgcg atggcaagat tgatctctac cagtgcgtgg tgccgaccac
     4441 ctggaacgcc agcccgcgcg atcccaaagg gcagattggc gcttatgaag cggcgctgat
     4501 gaacaccaaa atggcgatcc ccgagcaacc gctggagatc ctgcgtactc tgcacagctt
     4561 tgacccgtgc ctcgcctgtt caacacacgt gctgggcgac gacggtagcg agctgatctc
     4621 cgtgcaggtg cgttaacagc gaaggagaat catcatgcaa cagaaaagcg acaacgttgt
     4681 cagccactat gtctttgaag cgccagtccg catctggcac tggttgacgg tgttatgcat
     4741 ggcggtgttg atggtcaccg gatactttat cggcaagccg ctaccttccg tcagcggcga
     4801 ggcgacgtat ctgttctata tgggctacat caggttaatt cacttcagcg ccgggatggt
     4861 ttttaccgtg gttttgctga tgcggatcta ctgggctttt gttggcaatc gatactcccg
     4921 cgagctgttt atcgtgccgg tatggcgtaa aagctggtgg cagggcgtgt ggtatgaaat
     4981 ccgctggtat ctgtttctgg caaaacgtcc gagtgccgat ataggccata atcccatcgc
     5041 ccaggcggcg atgttcggct atttcctgat gtcggtcttt atgatcatca ctggttttgc
     5101 gctgtacagc gaacacagcc agtacgctat ttttgcgccg ttccgttatg tggtggaatt
     5161 tttctactgg acgggtggca actcaatgga cattcacagc tggcatcggc tggggatgtg
     5221 gttgattggc gcgtttgtga tcggtcatgt ctacatggcg ctgcgtgaag acatcatgtc
     5281 cgacgacacg gtgatctcca ccatggtcaa cggctaccgt agccacaaat ttggcaaaat
     5341 aagtaacaag gagcgttcat gagcgagcaa cgcgtggtgg tcatggggct gggcaacctg
     5401 ctgtgggccg atgaaggctt cggcgtgcgg gtggcggaac ggctgtatgc ccattaccac
     5461 tggcccgagt atgtggagat tgtcgatggc ggtactcagg gactgaactt gctggggtat
     5521 gtcgaaagcg ccagccatct gttgattctc gatgccattg actacgggct ggaacctgga
     5581 acgctgcgaa cctatgccgg agaacgcatt ccggcttatc tcagcgcgaa gaaaatgagc
     5641 ctgcatcaga acagtttctc cgaagtgttg gcgctggcgg atatccgcgg acatctgcca
     5701 gcacatattg ccctcgtcgg tctgcaaccc gcaatgctcg acgactacgg cggtagcctg
     5761 agcgaactgg cacgggagca actgcccgct gcggaacagg cggcgctggc gcagctggct
     5821 gcgtggggaa ttgtgccgca accggctaat gaatcgcgct gtctcaattg tgactgtctg
     5881 tcgatggaaa attacgaagg cgttcgcttg cgccagtacc ggatgacaca ggaggagcag
     5941 ggatgagcaa cgacacgcca tttgatgcgt tgtggcaacg aatgctggcg cgcggctgga
     6001 cgccagtcag tgaatcccgt cttgacgact ggcttacgca agcgccagac ggcgtggtgt
     6061 tattaagcag tgacccgaaa cgcacgccag aggtcagcga taatccggta atgattggcg
     6121 aattactgcg cgagtttccc gactatacat ggcaggtggc gattgctgac cttgagcaga
     6181 gcgaagccat cggcgatcgc tttggcgtct ttcgctttcc tgccacttta gtgtttaccg
     6241 gcggaagcta tcgcggcgtg ctgaatggta ttcacccgtg ggcggaactg ataaacctga
     6301 tgcgcgggct tgtcgaaccg cagcaggagc gtgcctcatg agcgaaactt ttttccatct
     6361 gctggggcca ggaacgcaac cgaacgatga cagtttcagc atgaatccac tgccgatcac
     6421 ctgtcaggtg aatgatgaac cgagtatggc ggccctggag caatgtgctc acagcccgca
     6481 ggtgattgcg ctgttaaacg agttacaaca tcaactaagc gaacgccaac cgccgttggg
     6541 cgaggtgctg gcagtcgatc tgttaaatct caacgccgac gatcgtcact ttatcaatac
     6601 gcttctcggg gaaggggaag tgtcagtgcg cattcagcag gctgacgaca gtgaaagtga
     6661 aatacaggag gcgatcttct gtggattatg gcgggtgcgc agacgtcgcg gcgaaaagtt
     6721 gctggaggac aaactggagg ctggctgcgc gccgctggcg ttgtggcagg cggcaacgca
     6781 aaatctcttg ccgacagatt cgctgttacc gccgcccatt gatggcctga tgaatggcct
     6841 accgttggcg catgagttac tggcacatgt acgtaacccc gacgcgcagc cgcacagcat
     6901 taatctgacg caattaccca tcagcgaggc tgatcggctt tttctctcac gtctctgtgg
     6961 gccgggaaat attcagattc gtaccattgg ctatggcgag agctatatca acgccacggg
     7021 gttacgccat gtctggcatt tacgctgtac ggacacctta aaaggcccgt tactggaaag
     7081 ttatgaaatc tgcccaatac cggaagtggt gctggcagcg ccagaagatt tggtcgactc
     7141 tgcgcagcgg cttagcgagg tatgtcagtg gctggcggaa gctgcaccga cgtaaaaaga
     7201 cggtaagtat cgctttcagt cttatgaata tcgcaatcgg cgaatacctc tggtcgtaga
     7261 gtttcaggat aaagagggag atctaccatt atcgggttat ttttctctct tcgcctacag
     7321 gagtgcgcat gtgggatgtc attgatttat cgcgctggca gtttgctctg accgcgctgt
     7381 atcacttttt atttgtaccc cttaccctgg ggctgatttt tttgctggct atcatggaaa
     7441 ccatttacgt ggtcaccggc aaaacaatct accgcgatat gacgcgcttc tggggtaagc
     7501 tcttcggtat caattttgct cttggcgtgg ctaccggcct gaccatggag tttcagtttg
     7561 gtactaactg gtcattctat tccaactatg tgggcgatat ttttggcgca ccactggcga
     7621 tggaagcatt aatggccttc ttcctcgaat ccacctttgt cgggctgttc ttcttcggct
     7681 ggcaacggct gaataaatac cagcacctgc tggtgacgtg gctggtggcg ttcggttcaa
     7741 atctctctgc gttgtggata ttgaatgcca acggttggat gcaatacccg accggtgcgc
     7801 attttgatat cgacaccctg cgtatggaga tgaccagctt cagcgagctg gtctttaatc
     7861 cggtcagcca ggtgaaattt gtgcacaccg taatggcggg ctacgtgacc ggggccatgt
     7921 ttattatggc gatcagcgcc tggtatttac tgcgcggacg ggagcgcgat gtcgcattac
     7981 gctcgtttgc catcggttcc gtcttcggta ctctggcgat tatcggtacc ctgcaactcg
     8041 gagacagttc tgcgtatgaa gtcgcgcaag tacaaccggt aaaactggcg gcgatggaag
     8101 gggagtggca aacggaacct gcacctgcac cgttccatgt ggttgcctgg ccggaacagg
     8161 atcaagagcg taacgccttt gccctcaaaa ttcccgcgct gctagggatc ctcgccactc
     8221 actcattaga taaacccgtg ccgggtctga agaatttgat ggctgaaacc tacccacgct
     8281 tgcaacgcgg acgtatggcc tggctgttaa tgcaggaaat atcgcaaggc aatcgtgagc
     8341 cgcatgtgtt gcaggcattc cgggaactgg aaggtgacct gggctacggc atgttgctct
     8401 cccgctatgc gccggatatg aatcatgtca cagccgcaca gtaccaggcg gcgatgcgtg
     8461 gcgcgatacc tcaggttgcg ccggtattct ggagtttccg catcatggtg ggctgtggtt
     8521 ccctgctgct actggtgatg ctgattgcgc ttgtccagac gctgcgtggc aaaatcgacc
     8581 agcatcgctg ggtgctgaaa atggcgctct ggagtttgcc gttgccgtgg attgcgattg
     8641 aagccgggtg gtttatgacc gagtttggtc gtcagccgtg ggcgatacag gacatcttac
     8701 cgacatactc cgcgcactcc gctttaacca caggacaact ggctttctca ctgatcatga
     8761 tcgtagggct ttacaccctg ttcttaatcg ccgaagtcta cctgatgcag aaatatgccc
     8821 gtctggggcc gagcgcgatg cagagtgaac aaccgacgca gcaacagggg taaaggagaa
     8881 aatcatgttt gattatgaaa cattgcgctt catctggtgg ctgctgattg gcgtgatcct
     8941 ggtggtcttt atgatctccg acggatttga catggggatc ggctgtctgc tgccgctggt
     9001 ggcgcgtaat gatgatgaac gccggatagt gataaacagc gttggtgcac actgggaagg
     9061 caaccaggtc tggttgatcc tcgctggtgg ggcattattt gccgcctggc ccagagtgta
     9121 tgcagcggcg ttttccggct tttatgtggc gatgatcctg gtgctgtgct cactgttctt
     9181 ccgcccgctg gcctttgatt atcgcggaaa aatcgccgat gcacgctggc gtaaaatgtg
     9241 ggacgccggt ctggtcatcg gcagtctggt gccgccggta gtcttcggta tcgccttcgg
     9301 caacttgttg ctcggcgtgc cgtttgcctt cacaccgcaa ttacgcgtgg agtatctcgg
     9361 cagcttctgg caactgctga cgccattccc tttattgtgc ggattgctca gccttgggat
     9421 ggtgattttg caaggtggcg tctggttaca actgaaaact gttggtgtga ttcatctgcg
     9481 ttcacagctg gccaccaaac gcgctgcact gttggtgatg ctgtgctttt tgctggcggg
     9541 ttactggctg tgggtcggta ttgatggctt tgtactgctc gcccaggatg ctaacggtcc
     9601 ttccaatccg ttaatgaaac tggtggcagt gctacctggt gcctggatga ataatttcgt
     9661 cgaatcgccc gttttgtgga tcttcccgct gctggggttc ttctgcccat tgctgacggt
     9721 gatggcgatt tatcgtggtc gcccgggttg gggattttta atggcgtcat tgatgcaatt
     9781 tggcgtgatt ttcacggcag gcatcacgct gttccccttt gtcatgccgt caagcgtgag
     9841 tccaatctcc agcctgacgt tgtgggacag cacttccagt cagctgacgc tgagcattat
     9901 gttggtaatc gtgctgatat ttttgcccat tgtgttgctc tacactctct ggagctacta
     9961 caaaatgtgg ggacgcatga caacagaaac tctccgccgt aacgaaaacg agttgtacta
    10021 aggagcagga acaatgtggt atttactttg gttcgtcggc attttgttga tgtgttcgct
    10081 ctccaccctt gtgttggtat ggctggaccc gcgtctgaaa agttaacgaa cgtaggcctg
    10141 atgcggcgca ttagcatcgc atcaggcaat caataatgtc agatatgaaa agcggaaaca
    10201 tatcgatgaa agcgatctta atcccatttt tatctcttct gattccgtta accccgcaat
    10261 ctgcattcgc tcagagtgag ccggagctga agctggaaag tgtggtgatt gtcagtcgtc
    10321 atggtgtgcg tgctccaacc aaggccacgc aactgatgca ggatgtcacc ccagacgcat
    10381 ggccaacctg gccggtaaaa ctgggttggc tgacaccgcg cggtggtgag ctaatcgcct
    10441 atctcggaca ttaccaacgc cagcgtctgg tagccgacgg attgctggcg aaaaagggct
    10501 gcccgcagtc tggtcaggtc gcgattattg ctgatgtcga cgagcgtacc cgtaaaacag
    10561 gcgaagcctt cgccgccggg ctggcacctg actgtgcaat aaccgtacat acccaggcag
    10621 atacgtccag tcccgatccg ttatttaatc ctctaaaaac tggcgtttgc caactggata
    10681 acgcgaacgt gactgacgcg atcctcagca gggcaggagg gtcaattgct gactttaccg
    10741 ggcatcggca aacggcgttt cgcgaactgg aacgggtgct taattttccg caatcaaact
    10801 tgtgccttaa acgtgagaaa caggacgaaa gctgttcatt aacgcaggca ttaccatcgg
    10861 aactcaaggt gagcgccgac aatgtctcat taaccggtgc ggtaagcctc gcatcaatgc
    10921 tgacggagat atttctcctg caacaagcac agggaatgcc ggagccgggg tggggaagga
    10981 tcaccgattc acaccagtgg aacaccttgc taagtttgca taacgcgcaa ttttatttgc
    11041 tacaacgcac gccagaggtt gcccgcagcc gcgccacccc gttattagat ttgatcaaga
    11101 cagcgttgac gccccatcca ccgcaaaaac aggcgtatgg tgtgacatta cccacttcag
    11161 tgctgtttat cgccggacac gatactaatc tggcaaatct cggcggcgca ctggagctca
    11221 actggacgct tcccggtcag ccggataaca cgccgccagg tggtgaactg gtgtttgaac
    11281 gctggcgtcg gctaagcgat aacagccagt ggattcaggt ttcgctggtc ttccagactt
    11341 tacagcagat gcgtgataaa acgccgttgt cattaaatac gccgcccgga gaggtgaaac
    11401 tgaccctggc aggatgtgaa gagcgaaatg cgcagggcat gtgttcgttg gcaggtttta
    11461 cgcaaatcgt gaatgaagca cgcataccgg cgtgcagttt gtaatgcata aaaaagagca
    11521 ttcagttacc tgaatgctct gaggctgatg acaaacgaag aactgtctaa tgcgtagacc
    11581 ggaaaaggcg ttcacgccgc atccggccac tttcagtttt actctttctc ggagtaacta
    11641 taaccgtaat agttatagcc gtaactgtaa gcggtgctgg cgcgtttaat cacaccattg
    11701 aggatagcgc ctttaatatt gacgcctgcc tgttccagac gctgcattga caaactcacc
    11761 tctttggcgg tgttcaagcc aaaacgcgca accagcaggc tggtgccaac agaacgcccc
    11821 acgaccgcgg catcactcac cgccagcatc ggcggcgtat cgacaatcac cagatcgtaa
    11881 tggtcgttcg cccattccag taattgacgc atccgatcgc gcatcagcag ttcagacggg
    11941 ttaggtggca cctgaccgcg agtaatcaca tcaaagcctc ctttgccaaa atgctggatc
    12001 actttgttga gctcatcttt acctgccaga tattccgaca agccatgttc attactcacg
    12061 gtaaacaggt tatgcgaata accacggcgt aagtcggcat caataaataa cactttttga
    12121 tcggactggg cgatcaccgc tgccagagtt gaactgacaa acgttttacc actgtctggc
    12181 gtcgcaccgg tgatcatcag aatgttattc tccgtctcca tcatagcgaa atgcagactg
    12241 gttcgtagcg cacgtacggc ttccacagca gaatccgccg ggttatccac cgccaggaag
    12301 gggatatttt tagtacgatg gcgctgctga ttagaaaata aatttttctt acgcagacgg
    12361 gtgcgtttat ccagccactc ggacattggg atagtggcat aaacgctgat gccgtgctct
    12421 tccagttgtt ccggggcttc tacaccacga cgcaacatcg cacgcgccag cacggcaccc
    12481 acagaaataa acaggccaag aataaaacca agcaccacat tcaacgcttt tttcggtttc
    12541 actggctgcg gctgagtgac tgccgggtcg ataatccgca cgttaccaat ggcactggat
    12601 ttcgaaatac tcaactcctg ctggcggtta agtaattgca gatataccgc acggcccgct
    12661 tctacgtcac gacttaaacg caacacttcc tgttgggtgg aaggcattgc cgatacccgc
    12721 ttattcaggc gtttgcgttc ttgctccagc gtctggcgtt tttccagcag cgcacgataa
    12781 gttgggtgat ctttcttata cagctgggag atctctgcct cgcggaaggt cagctcattg
    12841 agttgattat caacgttcac aatctgctca agaacggctt tggcttccag gttaaggtca
    12901 accgaatcgc gctgctggcg ataaacgttg agtttttctt ccgcctggtc cagctcgctg
    12961 cgcacttcag gtaactggcg ctgtaagaat tcaaggcttt gtgaatcctg cgccgcctgg
    13021 cgagcgatat tctgttgcaa atagttgtta gcgatgctgt tcagaatacg agtaatcaac
    13081 tggggatcat caccagtcat ggtaagttcc agcatcccgc tttctttact gcgttcgcta
    13141 acggtaaagg tttcctgcaa tgcgttaatc gcttccagtt cggtacgctg gctcaggaca
    13201 aactgtgttc ctggtttggc cttaatgtcc gcgatagtca gcgcaacgcc atctttttcc
    13261 agacgctggc cgaccatacc attgacggtg aactcttcac cttccagtgt atagtggccg
    13321 ttttccccaa ccgtgagtgt cagttgctga tcctgaccat tcagttgtgg aatatgcatc
    13381 cagctgatcg ccagctcacc tggtttttct ttggttaatc tcgcccagcc gcgacccaca
    13441 atcggaaaat acttctgctc aactatgtcg cgcagattca gttcagcaat ggttttaccg
    13501 agaatcatgc gcgattgcag cagttggatc tccggtgcag actcgggcga tgagttaggg
    13561 atcatatcgc tcaggccgct gagaatggcg ttgccctgtt tttgctcgac ctggaccaga
    13621 gtatctgcct gataaattgg tgtgcttaac agcgagtaag cgacagcgat cagcgtgaat
    13681 aacgcggtca cgctgataat aaacttacgg tgatcccata actcgccgac cagacgaagc
    13741 agatcgatct cattttcctg agtgctgcct ggtggcgtat tcatattttt agttgtcatc
    13801 gtattttata aactcaaatt accggctgag gcgcttcgcc cattcctgac tggcgcgctc
    13861 caacataccg tagacatgtt caaatgcgtc ctgactttta cgatagggat ccgggatctc
    13921 tttctgttcc agccattgcc caaacagcat tgtcttcccg cggacctcgg gagcaattgc
    13981 ggtaacctga gcaatatgtt ccgactccat tgccagaatc agatcgtaat ttcgtgccat
    14041 ctcagcagtg agcttacgtc cggcatgtcc ttcaagagat acgccatgat tagcggcaac
    14101 atctgccgct gtcgcatctg cagggtgttt taccagacca tgaacacctg ccgatttgac
    14161 ttttacgccc ggcagacgtt tacgcagcaa gcgctcgcca atgggcgaac ggcaaatgtt
    14221 gccggtacaa accaccagga ttgagttaaa ttttagttgg gccatgtttg aatccaccgt
    14281 gaagtctctg tcaggtcatg caccccggaa atcgtcggta ccagcagaga aatcacacgg
    14341 ttccaacgag ccagcggcgc ggtagtgaca taaacaatgt cgtaaggttc aagctggaat
    14401 tccgttccga gtatcattgc cgatgcgtcc ttcgcattca gctgataaat attggcgatt
    14461 ttgccattct gcttgttttg cgttgcacga ataacaaaaa tccccgtcgc atcagcgaca
    14521 tcctgattca taccctcagc gttacccagc gcttccgcca gggtcatacc gctgcggtcc
    14581 attttcagcg tgctctgttt acccacttca cccatcacga agactttcag atcatcgttg
    14641 cgcggaataa ataaaatatc gcccgggtgc agcagtttgt tctggcgcaa gtcgccacgc
    14701 tgcatcagcg catagaggtt cactttggtc tttacgccat tctgggtgag caccacgttg
    14761 cgccagtctg catctgcggt cagaccgccc gcggcgttaa tcgcatccat gatggtcaga
    14821 ggaatattgg taatcggctg ctgacccgat ttgctgactt cgccggtgac gtacgctttt
    14881 tgcgatcgga acgccgcgac gctgacatcg acttgcgggc tttcaatgac tgaatcgaga
    14941 cgcgctgtaa tctcgttgcg tacctgggta agtgttttcc cggccacttt caggcgacca
    15001 atataggggt agaaaatcgc accatcggcg ttaacccagt tgccggtatc actggcgcta
    15061 cggtattgcc ctgcgggggt ggttagttcc gggtgatccc agaccgtaac catcaacaca
    15121 tcaccaatcc cgatacgata ttcatagttt gcgatttgct gctccagctc cgggttagcc
    15181 tgcgacataa tcggtttggc gcgcaactga tcaatcaatc ctggcgtgac gggatagata
    15241 ttgaccattt tgtccaggtc atactgattg tcaggtagtt caatgacatc cttgttactg
    15301 gtcgataaat tctgaccagg aacaagggta catgctgtca aagacagaac agccaatgtc
    15361 agtaccgaaa acttaaaaat gttctttttc attggtttat atttatcagt caatgtaatg
    15421 ttatgaatta cgtcaggtta ttgtccaacc ggtacttctc gctctgccgt cattggatag
    15481 agctggtatt tgcgtgacag catttgtcca ccatcacgcg tcaatggtgt ccacgaaacc
    15541 accgcgcggt tgcggttagg tccaatggtc atcaagtcga atggaatcga gatataaaaa
    15601 cctttactaa agccgccttc gccgtagtca tctttagata cgttactgat tgccgcccat
    15661 acccctaccg ccacgccgct gtcaaagcgt ttggcgacgt cgatcgttgc gcctttatct
    15721 tttgccagat attgcccaac gctaagtttc atcagtacgc cattgagcgt cggcgggttc
    15781 cagtaagccg tcacgaaacc tgttggcgtg gaataatcgg tgaaacgcat catgttgtcc
    15841 cagttacgct gcttcacgta gttaacgtcc acacccagcg cccagctggc atctagcggg
    15901 cgataaagca gctcggaacc gacaccggca tacatcgttt ccagataacc gccatacacc
    15961 tggccataga aaccattgcc taagtcagca aaatagttcg cctgcaagtt gttgagataa
    16021 acgtcattgc gaacgtagtc acggatatgc gtgcgcacgc ggggcagggt agagtccgcg
    16081 ggcaacagcg aagacttaaa cttgtcgtag ttgttgtaaa tattggtgaa aataccgcca
    16141 tcaagcagca ggtggtcggt aaaccagtag cgggcactgg acatcagccc cagctggaac
    16201 atatagaaat cttccgggcc gcccagcgac tgtgaaagtg ttgggttgaa agagtagcta
    16261 aagcgatctt cacgaatacg gtagccccga ccaaaggcag aaagatcttc tgcttcaaca
    16321 cgttgttgtt gcagttgctc tgatttacca ggcgctgtac cagccagctg tttgcgcagg
    16381 ctggctacgt cggtttcggt agtcaccatc gccatatgct cgcgcttttg cgtcacgcta
    16441 atcttctcaa cgccttgcgg caggttattc accagaatcc ggttggcacg atcgaccgct
    16501 tcgcgagaat cacggtattt gtattgttga ccagacatat acagcgtctt atcgcgcagc
    16561 tgaatttccg gtgcttcaaa acctgcgttg tacttcagcg cggtaagttg gttcgctact
    16621 gtggtgtact gcaatccttc agattcaggc gcaggttgat atgccggttt tggcgtatcg
    16681 cgcagggcag ggcgcagatc gttgaaattg gtccgtaacg tgaagccaaa catcaacgtg
    16741 ttaccgcgtt cataactcag gttgagatct gcccagctgg cagcgcgata aactgcgccg
    16801 acgttgaaat ggcttgcctg aggcagtttg ccagcgaaat cattctggta attgttgcca
    16861 tcgtattcga gtttcagacg caggggattc cacggcgttt gatactcaat gccgccaaag
    16921 atggaagccg ggccacgaaa gatatcgcta aagctgatat cgcccgcatc gtgagactct
    16981 gcgcgatgac aatatttatc gctcacccgg caaaacgggt tggtaatatt gcccgcatta
    17041 ccggcgtagc cccatgccat cccgagggtg aaatcaaatg gccccgcttg cttgctggcc
    17101 accagatact caccgtcaaa cagacccgta ccagcaatat cacgtttacc aaacgccact
    17161 tgcggtagcc agtaaccttc ttcccacagg cgaagtttaa aatcgaatga tttgtctttg
    17221 tatgactgat cgccgctgaa atcttcccac tggctatatt tgcgtgtgcg cacatccgta
    17281 taacgaatgg tgccttccag ccatgggaac agcgccacgg aggtggagta gaaccggtat
    17341 tgatcgttat cccggtagtt gacgctgaat tcaccttccg gtgcgatgcg cgcatttggc
    17401 atctgcaaca atcctgtgcc gccaaagtct gattgcgacg aacccagcgg atccgggtag
    17461 gttaatactt cagcatgaca ggcactggag acggcaatgg ccaggcagct taaaagataa
    17521 gaattcttct tcatcagtca ggaacacgtt gcgttaaaac agacacaatc tggtcattta
    17581 aatcagcata tttttcgggc agtacatgcg cagaaaagcc caaccaaagc tggctgccag
    17641 gcggtggttc cacatgtctt ttattccaca gggcaacagg ggcgacgaca gtctcaccct
    17701 caggcgtaat caccataacg ttattcttgt ccgcgcctgc aaggcggggg tgatcttgca
    17761 gataatcggt aacgctacgg cctgcctgcc agggcaattg cccggcccca gaaaccgcac
    17821 ccagcagagt aatagtcacc ggacgctgta cggtatagag cgtatagtcc ccgaccaacg
    17881 ggggattgct gttctcatcc actcgtacaa aatcagggtc cagtttgacc ggtaagcgac
    17941 cagtgatatt gagattgagt aactgctggc gcacggattt aatcgttgcc gccacatcgt
    18001 cgtcagcttc tgcttcccag gaagcaagct gtgccatgac atgttggtag tctttaagcg
    18061 ctttagcttt cgccgcagag tcggtcagca aggcaccagg ccaccagagg cgatcgcgca
    18121 gttgcggttg tgtcaccaac tggacaacat tttccacggg gccaacggaa agtgtctgtt
    18181 gttcgccagg cagataaata gtcaccgttc cttgcgcaaa ggcatggggt gtcattacgt
    18241 aaagtacgct ggcaatgaaa tacgactgta atttattcat ggtgcgggtt taagaaacgt
    18301 catttctacg ggaataaccc ctgcgcctaa catttgccga ctttgacgaa cctggccagt
    18361 agccgaatcg acccagaatg tgttttgcca ctgtttttct ggccgcgtgg aagtcacctc
    18421 ttcctgccag acattacaca gcacattgcg acccgcaata ttcagcgtct ctaatccagc
    18481 aaaggaaaaa gtagaactca gggtcgcaga gcgaaaatgg ttgtcttcac tccagagaat
    18541 atcgcgcgtc cagcggctgc catcttttat tgccagcgcg ttacgcagag ggtcctgccc
    18601 ggaatttgtc acttccagca gattattatt aagtttgacg gtttttaaaa gacgtccatt
    18661 gtgggtaacc agcatggcgt tatcctggga taaccatttg ctttgttctt gttctatata
    18721 tcccagtacc acaaagattc gttgcccttc attaaggcgt aaatacatcg tggaataagg
    18781 caacgcctgg atctgctgat ccgctacggt gatatcctga ttatcgaaaa ggctggcgcg
    18841 aaatgtatcg accatacttt gctggctgtg cgtacatccc gcaagaaata gtgcgaaaat
    18901 cgataaaata agagggcgca cctttaatcc ttgaaaggtt aattaaaaaa atgcaaatct
    18961 ctaaagcccg gtacgaatat cgcaaccggg tatcatttaa ctttaatttc gacgcgttaa
    19021 agcagtatta gcgtgtagtg gacgtggtgg ttgaggtgtt atgagaaccg ccatcgccac
    19081 tgtcatttgc tgcaacaact gccaacgtcg ccacaccaac agccgttgcc acaccgacaa
    19141 ctgtcgccgt gccgacagcg ccagcagcca cgccaccagt ggtagtagca gtcgtggttg
    19201 cgccggtagt ggtcgttgtg gtgccagtag tacccgtcgt tgcctcggtc ccgtttgttg
    19261 cttcaggggc agcaaacgca gctggcgtcg tcaacatgaa ggccataagg attgcagaaa
    19321 gcttgtgttt cataactttt cctttattca tcgcatggac aatacgtacg cttgagatca
    19381 ggattgtcta ctttgagtat aacatccttg tgaaatctcc ctttcctcat accgcttgca
    19441 ctatatactg cgggtaggaa aggcgcgcag agggaaatat aagattgtta actatccccc
    19501 tctggaaata cgacttgtga gcataagata aatcttagca aaatacaaat taaagataag
    19561 gaaattgtta cgaaagctat taattattgt tggtaatata gtttcaagtg gtactataac
    19621 tggctgttaa atggcattta taactattag gtgcagagat attcgcttaa atggattagt
    19681 ttctttgtgg aaaagtaact gatgttataa tgtatgatgt tgactattgc actattccaa
    19741 taaaaccagt cagctttaaa caagcagcgt catattaaga gagataaaca tttgccgctg
    19801 ttggtcctcg caggccattt acgcggcaaa attccacacg taatcctggt ataagcactt
    19861 ctgcgtcgcg gggagtgaat gcggaaatat ggacctgaac ttctttacga ccgtcggagg
    19921 ggataatgaa tcctttgccg cttttgcgat caaaggtttt gacaattcct gtcattttac
    19981 gggacaaaca aattccttac tgaaaatact gcgctgcact atacggggtt aataaaataa
    20041 agccagcgat atttaagacc gccggacggc taaaataaaa tttgcttaat ctcaattatc
    20101 atgcgttaat agctgcgtcg gtttgaaaga cagacagcat acaaagtagt ttactaaagc
    20161 agttctcatt atcaggcatt atccccttct tttgagtctc tctcctgaac actaagtagt
    20221 ttctgtatta aagccctgtt tgccgaaagg cccaaaatga aggaagtaaa atatgtctaa
    20281 taaaatgact ggtttagtaa aatggtttaa cgcagataaa ggttttggct ttatcactcc
    20341 tgatgatggc agcaaagacg ttttcgtcca tttcaccgcc atccagagca atgaattccg
    20401 cacgctgaac gaaaatcaga aagttgaatt ttctattgag caggggcaac gtggccccgc
    20461 ggcagcgaac gttgttacgc tctaaggttg ccgttattac tcaacatctc catttccgct
    20521 gtccatgttg tcatggttca cagtaccgca catcggcatt cgatgtgacg gagcgaaacc
    20581 ctttgggcgc taagtgtatt ttttgtaaat cgacgatgat cacctttgat aacgtcgcgc
    20641 tgcaaatacg cactgaccat gtgccgctgg atttcacaaa ataatatcag gctccctcgt
    20701 ggagcctttt ttatatctgc cttatttttc ttcaacgctg tatgtatagt aagcgataac
    20761 ctgttgatta ttgaatcttt cggggagatg gcttataaca tttcttacct gaccagggta
    20821 ccgggaacca acaccttact ggcgtgttgc tgtcttttaa gaccagaaga ggttaacagt
    20881 gaatattgaa gagttaaaaa aacaagccga aacggaaatc gccgacttta tcgcgcaaaa
    20941 aatcgccgag ctgaacaaga atacagggaa agaagtctct gaaattcgct tcaccgcacg
    21001 agaaaaaatg accgggcttg aaagttatga tgtcaaaatc aaaataatgt gattttgtga
    21061 acatcacccc gtgcgaggtg atgttccgct tgttgctaat ttagtgacca atcattggcg
    21121 cttgtggaat taagcgtcgg tacaattcct ccggcaccgg gctttgccat actcccgcat
    21181 acattgcgta accaatcacc gcgaacataa tccccagaac cagtagcgtc attaaccagc
    21241 cagacaacgc aaaagctttt ttatttgccg caggtttttg cagtgaaaag gtcaatgttg
    21301 aggctaccgg acatgactct acgcaagtca tacagccggt acattccact gttcgtacct
    21361 gaattaattt atctaccggg atccgtgatg ggcaattttt tgcgcatttg ccacagccga
    21421 tacaactttc ggcattgcga cgaatcttaa acggcgataa tagcgaaacc acgcccatca
    21481 gcgcgccata tgggcaaaga taacgacacc aggcatggcg aataaacagg ctggcaatca
    21541 gcaaaacggt cacgctgatt aatgtcgcgg tccccatatg acgaaagaaa tcgagcattt
    21601 ttacgtccat caccacgctg tagggcgaca acataaaata gtgaatcgcc tgagcgggca
    21661 tcaataacgc gatatagaga aaaaaactca acagcaaata cttcacgccg cgcagaggaa
    21721 tatccagcca gcggggaagg acacattgcc gaccaaacag tttgttaccg agatcgccga
    21781 ttaattcaga aagcgtacca accgggcata accatgagca aaaggccttt ttgagtaata
    21841 gactgatgac gataaaagcg accaataaca gcatcgcggc ggcgtggacg gacggtaact
    21901 gacctgttgt aaggctatat ttcagattca tcagaccggc aatcggtagc cagccttcga
    21961 tacctcccgg tctggcgaca aatgtcgtgc tacttgccgt ttcgtaatag cgcacccaat
    22021 accagaacgt gatggcaata taaatattca ttgccaacag taataattgc gtcgctttac
    22081 gccaggtcgt ggcattgcgc cagtcattcc acggtaattt gccgcccgtc gtgcctggcc
    22141 gccgctgcca gcgggttctt tttttctctg ccatgatttt gccagtccgt taagttgcat
    22201 accaaatgcc actattctag ttgttcttaa ctggctgata ttgattcaaa tcgcgttcag
    22261 gtctttctta ttcaaccatg cttccagatc ggcaacactg cgtgtaattt cttcgtgtgg
    22321 aaggggggca gataatggct gctgctccag ttgtgcgcat agctggctgg cgatacgcat
    22381 tcccaaactc gagcaactgc tttttagctg atgcgcggca cgctttattt tctcgctatc
    22441 ctgactggcg cgggcaatgt cgatttcatc gagaagcggc agggcatgtt gtgtaaataa
    22501 taccagccat tcgtggatct tctccgtccc cattaactga gcatcttcat tgagttgcga
    22561 tacatccagc gattgatcgt tattgacttg cagttggaga tagtgcgcca gtaactgacc
    22621 gagcacttca cgcggcaccg gtttagggat aatcccgcgg aataatgaac tggtacgctg
    22681 gcgcagcgtt tcgtcaatga catgggcgct aaagccaatc aaaaccagcg acggatattg
    22741 ctgtgccagt tgtcgggcaa gcgtaatgcc gtcgacatcc ggcagatcaa aatccaccag
    22801 tgcggcagca aacggttcgc tattttgcag tgtctctaaa gcctgcgcgg cattgccaac
    22861 agcaacaacc tgcgcaccac tggtgttcag catctcaacg gtaattcgct gggttagcgg
    22921 gttatcttca attaacagca aacgtaaacc gtcaagacgc accgcctgat tgactgtttt
    22981 tggcacgggt gccgtggcaa cacgtaacgg caagcgtaaa caaaaacagc ttccaacctc
    23041 cggcgtgctg gtggcgctca gttcgccgcc catcgcctgg gccagacggc tactgatagt
    23101 cagccccagc ccggtgccgc cgcgtttgcc gcttacctgc acaaatggct ggaagatttc
    23161 tgccagtttc gcgggatcaa taccgcagcc gctgtcttcc acttcgacca gccattgctc
    23221 gccatcagtg cgactacgca ggataatgta cccttcgtca gtaaaacgca gggcgttgct
    23281 caacaggttg gttataacct gacgaatacg tcgtggatcg cccattaacg cgcacggcat
    23341 atcatcggca attgccgttg ccaggcgaat cgggcgacct ttcacccgtc cgctcattaa
    23401 ttgcagggta ctttccagca gcgggcgcgg ttcaaagggc tcatcgctga ccgaaacatt
    23461 cttgccacct gcttcgatag cggaataatc gagaatatcg ttgaggatgg tcagcaacga
    23521 ttcaccagag tcagtaattg cccgcaaatc atcacgctgg gcgttaagtg cggggttatc
    23581 tgccagcagt tgagcagtgc cgagaatacc gtacagcggt gtgcggatct catggctcat
    23641 cgccgccaga aacgccgatt ttgcctggct ggctttttct gcttccgccc gtgcctgtcg
    23701 gtgttctatc accagttcct gcaattcagc tgtacgcgct ttgacctgcg ccgccagctg
    23761 ttcgcggtgg cgattcagtg catgaacatt gctgcgaaac gcatccatca gccgcccgat
    23821 ggtatccagc tcccgtacgc cagcggtttc cgggaaaggg gagtcaatat caccgtccag
    23881 cagccgttgc agcgcctgcg tttgttcggc aagtggacgc gtgactgagc gataaaccac
    23941 gcgccaaagg atcagaatca gtgcgcaaag tgaaaccatc cccagcaata acaggctgta
    24001 ttgcccgcgt gcactggctt tttccagatg cgccagtccg tgctgattac gcagctcaat
    24061 ggtgtcgacc agctgactga cttcgctgct aaactgcgcg aactgggcga tgttattttg
    24121 tgcgagagtt tgtaggtgat tgctgatttc actgtcctgc tgatacagcg ccagcaaatc
    24181 gctatattgg ctaacggtag ttaacgttgt tgcgacctgc gcacgaacac ccggatcttc
    24241 aatgcgtatt tgccgacgtt gcagaatttt caccgcatta ttgagctgct tttccagcgt
    24301 tggtgcattt ttctggatct gctccagccc cagattcatc accatttgct gcacccgcag
    24361 agcgctaagg cgcagttcat tcatctggtt aacatactca agatcgatat caatcagccg
    24421 atcgagtgca ctttcagcag cctgacgctg atcttgttcg atcaaatcgt aaatcccggc
    24481 ctgggtcgct ccagcggaag ttgtcgcatt attcgcctga ccttgcgcca ggcgtgcgat
    24541 ctcatcggcg gcagcgacta tctgctgact gagttgccgt tgttgctggc gtagttgcag
    24601 acgccgcccc accagttccc cttgctgacg taatgaacgg gagatctcct gctcctgttg
    24661 ttcaatagcg gtggtatcaa aaccttgttc ccgtaacgct tgcagcaacg cattaatctt
    24721 caggctttgt gcggtgagca ttcgcccctg cgcctgccac atcttttcgt tatcggcact
    24781 ggtcaggttc tgcgcggcga aaagttccca ggcgctggct tcgctcaact ggcgcgccat
    24841 attcatggta ggaatcaatg cctgagtgtt gtctttttcc acctggctga taaagcgcag
    24901 gttgtaccat cccaccaggg tactggtcag ggttaacagc gccatcaggg caaagcccat
    24961 ccagagtctt cgggtcaggg ttaaattcac ggtcggtgca cttaaggtga aaaaggttga
    25021 gtcgcaaagc ggaatgcatc tagcataaag ccttattatt gatgaggcta tcatgcgcgt
    25081 actgctattt ttacttcttt cccttttcat gttgccggca ttttcggctg ataacctgtt
    25141 gcgctggcat gatgcgcagc atttcacggt gcaagcctct atgccgctta aagccaaacg
    25201 cgcatggaaa ctgtgcgcgc tttatcccag cctgaaagat tcatactggt tatcgttgaa
    25261 ctatggtatg caggaggcag ctcgccgtta tggtgtggat ttaaaagtgc tggaggcagg
    25321 cggctacagc cagttggcta cccagcaagc acaaatcgac cagtgtaaac agtggggcgc
    25381 agaggccatt ttactcggta gtagcacgac ctcatttccc gatctgcaaa agcaggtagc
    25441 aagtctgccg gtgatcgaac tggtaaatgc tattgatgct ccccaggtga aaagccgcgt
    25501 tggtgtaccc tggtttcaga tgggctatca acctgggcga tatttggtgc aatgggcgca
    25561 cggtaaacca ctgaacgtgc tgttgatgcc cggacccgat aatgccgggg gcagtaaaga
    25621 gatggtcgag ggttttcgcg cagccattgc cggaagcccg gtgcgtattg ttgatattgc
    25681 gcttggtgat aacgatattg aaatccagcg taacctgttg caggagatgc tggaacgcca
    25741 cccagaaatc gacgtcgttg ccggaacggc cattgcggca gaggcggcaa tgggtgaagg
    25801 gcgtaacctg aaaacgccgc ttaccgtggt gtcgttttat ctttcacatc aggtgtatcg
    25861 cgggctgaag cggggaagag tgattatggc tgccagcgat caaatggtct ggcaggggga
    25921 actggcagtt gagcaggcca tcaggcaatt acaggggcaa tcggtgtctg ataatgtcag
    25981 cccaccgatt ttagttctga cgccgaaaaa tgccgaccga gaacatatcc gccgctcgct
    26041 gtcaccaggg ggatttcgtc cggtctattt ttatcagcac acatcagcgg ctaagaaata
    26101 accttcacca tgttgcgtca ccagtaaatc cgcgctgagt ttatgacgta aacgacgaat
    26161 taacacatcg acggtgcgca ggtcagggtt ttccacccga cgcgcagaaa gcatacgtag
    26221 cagacgttca cggctgagaa tttcgcccgg attcgtcaca aatgccacca acatttcata
    26281 ctctgcgcgg gtcagtttaa tcggctcgcc atcccgctcc agcgtatggc gcgacacatt
    26341 caggcaataa ccggcaaagc gatagcagtt gtcctgagtg tgcggttgag cttgtcgcgc
    26401 gaggtcgatt cgccagagca gatttttcac ccgtactacc agttcgcgca gttccagcgg
    26461 tttggtgacg taatcgtctg cgcccatttc cagcccaaca atacggtcaa tccgatcgct
    26521 gcgtccggta accagaataa tccccaccgt tgagcgttct cgcagggcgc gggttaacat
    26581 caggccattt tcatcgggta agttgatatc cagcagaatt aaatctaccg gctgattctg
    26641 cataatttcc cgcagcccgg caccgctcgc tgtaacggaa acggtatacc cctcctgagt
    26701 gaagtaagat tgtaatcgcg cctgggtaac cggctcatct tcaacaataa caatgtgatg
    26761 tggcatcaga gggttttact cattctgttc atatctgttc atattctgcc gtaagccgtt
    26821 catcctgacc agtgccgctg ttcatatttg ctcattaaga tcgcttcact aaaccataat
    26881 tttacagggg ctattatgcg gaaactctgg aacgcgctac gccgacccag tgctcgttgg
    26941 tcggtactgg cgctggtcgc aattgggatt gtgattggca ttgcgctgat tgtattgcca
    27001 cacgttggga tcaaagtcac cagcacaacc gaattttgtg tcagttgcca cagtatgcaa
    27061 ccggtgtatg aagaatataa acagtcggtg catttccaga acgcctccgg cgtgcgagct
    27121 gaatgccatg actgtcatat cccgccggat attccaggca tggtgaagcg caaactggaa
    27181 gcgagcaatg atatctacca gacctttatt gctcactcca ttgatacacc tgaaaaattc
    27241 gaagccaaac gcgcggaact tgccgagcgt gaatgggcgc gaatgaaaga aaacaactcg
    27301 gcaacctgcc gctcctgcca taactacgat gcgatggatc atgcgaagca gcatcctgaa
    27361 gcggcacgtc agatgaaggt ggcagcgaaa gataatcaat cctgcatcga ctgtcataaa
    27421 ggtattgccc accagttacc ggatatgagt agcggcttcc gtaagcagtt cgatgagctg
    27481 cgcgccagtg ctaatgacag tggtgacacg ctgtactcta ttgatattaa gccaatttat
    27541 gcggcgaaag gcgataaaga tgcctctggt tctctgctgc ctgcttcgga agtgaaagtc
    27601 cttaaacgtg acggcgactg gctgcaaatt gaaattaccg gctggacgga aagcgccgga
    27661 cgtcagcgtg tactcaccca attcccaggt aaacgcatct ttgttgcctc gattcgtggt
    27721 gatgtgcagc agcaggtaaa aacgctggag aaaaccaccg ttgccgacac caataccgag
    27781 tggagcaagt tgcaggccac cgcatggatg aagaaaggcg acatggtgaa cgatatcaaa
    27841 ccgatctggg cctatgcgga ttcgttgtac aacggcacct gtaaccagtg ccacggcgca
    27901 ccggaaatcg cccactttga cgctaacggt tggatcggca cgctcaacgg catgattggc
    27961 tttaccagtc tcgataaacg tgaagaacgc actttgttga aatatctgca aatgaatgcg
    28021 tctgacaccg caggtaaggc tcacggcgat aagaaggaag aaaaataatg aacaataacg
    28081 atctctttca ggcatcacgt cggcgttttc tggcacaact cggcggctta accgtcgccg
    28141 ggatgctggg gccgtcattg ttaacgccgc gccgtgcaac tgcggcgcaa gcggcgactg
    28201 aggctgtcat ctcgaaagag ggcattctta ccgggtcgca ctggggggct atccgcgcga
    28261 cggtgaagga tggtcgcttt gtggcggcaa aaccgttcga actggataaa tatccgtcga
    28321 aaatgatcgc cggattgccg gatcacgtac acaacgcggc acgtattcgt tatccgatgg
    28381 tacgcgtgga ctggctgcgt aagcgccatc tgagcgacac ctcccagcgc ggtgataacc
    28441 gttttgtgcg cgtgagctgg gatgaagccc tcgacatgtt ctatgaagaa ctggaacgcg
    28501 tacagaaaac tcacgggccg agtgccttgc tgaccgccag tggttggcaa tcgacgggga
    28561 tgttccataa cgcttcgggg atgctggcga aagctattgc cttgcatggt aatagcgttg
    28621 gtacgggcgg agattactct accggtgctg cgcaggtgat cctgccgcgc gtggttggct
    28681 caatggaagt gtatgaacag caaacctcct ggccgctggt attgcagaac agcaaaacca
    28741 ttgtgctgtg gggctccgat ttgctgaaaa accagcaagc gaactggtgg tgcccggatc
    28801 acgatgttta tgaatattac gagcagctaa aagcgaaagt cgccgccggt gaagttgagg
    28861 tcatcagcat cgatccggtt gtcacatcca cccatgagta tctggggcgc gagcatgtga
    28921 agcacattgc ggttaacccg caaactgacg tgccgctgca actggcgctg gcacatacgc
    28981 tgtacagtga aaacctgtac gacaaaaact tcctcgctaa ctactgtgtg ggttttgagc
    29041 agttcctgcc gtatctgctg ggtgagaaag acggtcagcc gaaagatgcc gcatgggctg
    29101 aaaaactgac cggcatcgat gccgaaagca ttcgtgggct ggcgcggcag atggcggcga
    29161 acagaacgca gattattgct ggctggtgcg tacagcgtat gcagcacggt gaacagtggg
    29221 cgtggatgat tgtggttctg gcggcgatgc tggggcaaat tggcctgcca ggtggtggct
    29281 ttggttttgg ctggcactat aacggcgcag gcacgccggg gcgtaaaggc gttattctga
    29341 gtggtttctc cggctctacg tcgattccgc ctgttcacga caacagtgac tacaaaggct
    29401 acagcagcac tattccgatt gcccgtttta tcgatgcgat cctcgaaccg gggaaagtga
    29461 tcaactggaa cggtaaatcg gtaaaactgc cgccgctgaa aatgtgtatt tttgccggaa
    29521 ctaacccatt ccatcgccat cagcagatca accgcattat tgaaggctgg cgcaagctgg
    29581 aaacggttat cgccatagat aaccagtgga cctcaacctg ccgctttgcc gatatcgtac
    29641 tgcctgcgac cacgcagttt gagcgtaacg atctcgacca gtacggcaac cactccaacc
    29701 gtggcattat cgccatgaaa caggtggtgc cgccgcagtt cgaggcgcgc aacgactttg
    29761 atattttccg cgagctgtgc cgtcgcttta atcgcgaaga agcctttacc gaagggctgg
    29821 acgaaatggg ctggctgaaa cgcatctggc aggaaggtgt acaacaaggc aaaggacgcg
    29881 gcgttcatct gccagcgttt gatgacttct ggaataacaa agagtacgtc gagtttgacc
    29941 atccgcagat gtttgttcgc caccaggcat tccgcgaaga tccggatctc gaaccgctgg
    30001 gcacgccgag tggcctgatt gagatctact cgaaaaccat cgctgatatg aactacgacg
    30061 attgtcaggg gcatccgatg tggtttgaga aaatcgaacg ctcccacggt gggcccggct
    30121 cgcagaagta tccgttgcat ctgcaatctg tgcatccgga tttccgactt cactcgcagt
    30181 tatgtgagtc ggaaactctg cgtcagcaat atacggtagc gggtaaagag ccagtgttca
    30241 ttaacccgca ggatgccagc gcgcgcggta ttcgtaacgg tgatgtggta cgcgtcttta
    30301 acgctcgcgg tcaggtgttg gcaggggcag tagtttctga ccgctatgca cccggcgtgg
    30361 cacgaattca cgaaggggca tggtacgatc cagataaagg cggcgagcct ggtgcgctgt
    30421 gcaaatacgg taaccccaac gtgctgacca tcgacatcgg tacttcgcag ctggcgcagg
    30481 cgaccagtgc gcacactacg ctggtggaaa ttgagaagta caatggagca gtggagcagg
    30541 tgacggcgtt taacggccct gtggagatgg tggcgcagtg cgaatatgtt cccgcgtcgc
    30601 aggtgaaatc atggccacgc tgacagcaca acagattgcc tgtgtttacg cctggctggc
    30661 gcagttgttc tcccgtgaac tggacgatga acaactgacg caaatcgcca gtgcgcagat
    30721 ggctgaatgg ttttcgttgc tgaaaagcga accgccgctg gctgcggcgg tgaatgagct
    30781 ggaaaactgt attgccacgc tgacagtacg tgacgatgcc cgtctggaac tggccgctga
    30841 cttttgcggc ctgtttttga tgactgacaa gcaggcggcg ctgccgtatg catcggccta
    30901 caaacaggac gagcaagaga ttaaacgctt gttagttgag gcagggatgg aaaccagcgg
    30961 caatttcaac gaaccggcag atcatctggc gatctatctc gagttgttga gtcatctgca
    31021 tttttcgctg ggagagggga ccgttcctgc gcgaagaatc gacagtttgc ggcaaaaaac
    31081 actgacggcg ctgcggcaat ggttaccaga gtttgctgcg cgttgccatc agcatgacag
    31141 ctttggtttt tacgcggcac taagccagtt attgctggtg ttagtggagt gcgaccacca
    31201 aaacagataa cgtcgtttgt gcgcctgaaa agacgcgttt agcgtcgcat caggcattat
    31261 ggggcagttg ccggatgcgt catcacatcc ggcaaacaca catcacggat gagctacaaa
    31321 ccgggaaagt cgctggcgca gcaggcggtt ttcctgcttc aggtgcgcaa tatcatccat
    31381 taacgtcagc gccaccgcga tcccaggcca gtccagagcc agttcatgac gcaggcgtac
    31441 tgcgcgttgc accacgatgg cggcatggtc gtcaaatacc caggttgttt cctggatctc
    31501 acgcggttca accaccccca aaccgacaat ttcattcaac tcctcttcag agatgccggt
    31561 atgcaggcaa aattcggtaa tagtaaaagt caccgtaaca ttagccatta tgctttcccc
    31621 caatctttac gtggatcaaa agacgactgg gcgtctgcca gttgctgcca cagcgcggca
    31681 gtgttttcat ccggtttcgg cggcatcacg attttcagta ccgcatacag atcgccggtc
    31741 tgttttttgc tcaccagacc tttgccttta acgcgcaatc gttgcccggc ctggctgcca
    31801 ggcgggatag tcagcaaaat gctttctttc agtgttggaa cggtgacttt agcacccagc
    31861 gccgcttccc acgggctaac cggcaccaca atttccagat cctggccgac aatatcgaac
    31921 agcggatgtg gcgcaatatg aatcaccagc cacaaatcgc catttggacc gccattttcg
    31981 cccggcgtcc cctggccttt caggcggatg cgttgaccat tgccgacgcc cgccgggatc
    32041 ttcacattca gcgttttcgg gatttcctgt tcgatcatgc caaaggcgtt ataaaccggc
    32101 aggttatagc tgatggtacg cttatgctca gtcagcgttt cttcgaggaa taccgccact
    32161 tcgatttcaa tatcgtggcc gcgtgtggcg gggcgttgac ggctctggcg ggcatgctga
    32221 ccgaaaattg acgagaagat atcgtcaaaa tcttcggcgt taaaactctg accgtcgcca
    32281 tgctggaact gacggttaaa ttgcggatcg ttgcgatgtt gccacatctg atcatactca
    32341 gcgcgacgtt gttcatcact taacacttcc caggcttcag cgacctcttt gaagcgggct
    32401 tcggcatccg gttctttgct gacatcagga tggtatttgc gggcaagtcg acgataggcg
    32461 gtcttgattg tcttgagatc gtccgtcggt ttcacgccca tgatggcgta ataatccttt
    32521 aattccatag cgttatctcg cgtaaatcaa cacaaattga aggaacccct gtaaggtaac
    32581 tcctataagt gtagggtaat cctcaaaatt tcatatgcca acacagaata tgttattgaa
    32641 atcatcgcgg agaggaggtc gcctcaaggt ggggtgctga acatatttta aacaggtgaa
    32701 aaagggtgag cgatttt
//
LOCUS       DAZKIV010000033        32555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZKIV010000033 DAZKIV010000000
VERSION     DAZKIV010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.033"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..1832)
                     /gene="ycaO"
                     /locus_tag="ACHOT6_001616"
     CDS             complement(72..1832)
                     /gene="ycaO"
                     /locus_tag="ACHOT6_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFP9946853.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGKAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            1962..2654
                     /locus_tag="ACHOT6_001617"
     CDS             1962..2654
                     /locus_tag="ACHOT6_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFP9946854.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLCGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            2853..3941
                     /gene="serC"
                     /locus_tag="ACHOT6_001618"
     CDS             2853..3941
                     /gene="serC"
                     /locus_tag="ACHOT6_001618"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFP9946855.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            4012..5295
                     /gene="aroA"
                     /locus_tag="ACHOT6_001619"
     CDS             4012..5295
                     /gene="aroA"
                     /locus_tag="ACHOT6_001619"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFP9946856.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            5464..6228
                     /gene="ycaL"
                     /locus_tag="ACHOT6_001620"
     CDS             5464..6228
                     /gene="ycaL"
                     /locus_tag="ACHOT6_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFP9946857.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            6401..7084
                     /gene="cmk"
                     /locus_tag="ACHOT6_001621"
     CDS             6401..7084
                     /gene="cmk"
                     /locus_tag="ACHOT6_001621"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFP9946858.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            7195..8868
                     /gene="rpsA"
                     /locus_tag="ACHOT6_001622"
     CDS             7195..8868
                     /gene="rpsA"
                     /locus_tag="ACHOT6_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFP9946859.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            9028..9312
                     /gene="ihfB"
                     /locus_tag="ACHOT6_001623"
     CDS             9028..9312
                     /gene="ihfB"
                     /locus_tag="ACHOT6_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFP9946860.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            9519..11783
                     /locus_tag="ACHOT6_001624"
     CDS             9519..11783
                     /locus_tag="ACHOT6_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFP9946861.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHYGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            11820..13568
                     /gene="msbA"
                     /locus_tag="ACHOT6_001625"
     CDS             11820..13568
                     /gene="msbA"
                     /locus_tag="ACHOT6_001625"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFP9946862.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            13565..14551
                     /gene="lpxK"
                     /locus_tag="ACHOT6_001626"
     CDS             13565..14551
                     /gene="lpxK"
                     /locus_tag="ACHOT6_001626"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFP9946863.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            14588..15820
                     /gene="ycaQ"
                     /locus_tag="ACHOT6_001627"
     CDS             14588..15820
                     /gene="ycaQ"
                     /locus_tag="ACHOT6_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
                     /protein_id="HFP9946864.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRQASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYLLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            15872..16054
                     /gene="ycaR"
                     /locus_tag="ACHOT6_001628"
     CDS             15872..16054
                     /gene="ycaR"
                     /locus_tag="ACHOT6_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFP9946865.1"
                     /translation="MDHRLLEIIACPVCNGKLCYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            16051..16797
                     /gene="kdsB"
                     /locus_tag="ACHOT6_001629"
     CDS             16051..16797
                     /gene="kdsB"
                     /locus_tag="ACHOT6_001629"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFP9946866.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            16951..17844
                     /locus_tag="ACHOT6_001630"
     CDS             16951..17844
                     /locus_tag="ACHOT6_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFP9946867.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(17821..18600)
                     /gene="elyC"
                     /locus_tag="ACHOT6_001631"
     CDS             complement(17821..18600)
                     /gene="elyC"
                     /locus_tag="ACHOT6_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFP9946868.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPHEQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGSSGEPRQE"
     gene            18736..19521
                     /gene="cmoM"
                     /locus_tag="ACHOT6_001632"
     CDS             18736..19521
                     /gene="cmoM"
                     /locus_tag="ACHOT6_001632"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFP9946869.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDHVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            19518..20840
                     /gene="mukF"
                     /locus_tag="ACHOT6_001633"
     CDS             19518..20840
                     /gene="mukF"
                     /locus_tag="ACHOT6_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFP9946870.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            20821..21525
                     /gene="mukE"
                     /locus_tag="ACHOT6_001634"
     CDS             20821..21525
                     /gene="mukE"
                     /locus_tag="ACHOT6_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFP9946871.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            21525..25985
                     /gene="mukB"
                     /locus_tag="ACHOT6_001635"
     CDS             21525..25985
                     /gene="mukB"
                     /locus_tag="ACHOT6_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFP9946872.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIAELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            26246..28093
                     /gene="ldtD"
                     /locus_tag="ACHOT6_001636"
     CDS             26246..28093
                     /gene="ldtD"
                     /locus_tag="ACHOT6_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9946873.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            28274..28822
                     /locus_tag="ACHOT6_001637"
     CDS             28274..28822
                     /locus_tag="ACHOT6_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFP9946874.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            28849..29496
                     /gene="gloC"
                     /locus_tag="ACHOT6_001638"
     CDS             28849..29496
                     /gene="gloC"
                     /locus_tag="ACHOT6_001638"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFP9946875.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLTALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVR"
     gene            complement(29718..30908)
                     /gene="aspC"
                     /locus_tag="ACHOT6_001639"
     CDS             complement(29718..30908)
                     /gene="aspC"
                     /locus_tag="ACHOT6_001639"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFP9946876.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(31093..32181)
                     /gene="ompF"
                     /locus_tag="ACHOT6_001640"
     CDS             complement(31093..32181)
                     /gene="ompF"
                     /locus_tag="ACHOT6_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFP9946877.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQLLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNISGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         7950 a         7891 c         8724 g         7990 t
ORIGIN      
        1 ggcattaaaa aaacgccccg taatataact cagactaatc attaagccta cattgcgtag
       61 gctatttgat tttattttgc ccagaatgct gctttggcgc gctgcagctt ttcgtaggcc
      121 ttcaacaacg actgatgtgc agcaaacgcg tgcagatcgc tatctaccgg ttgcaggccg
      181 taaaacgccg ctttgccgct cattgccgca ctggcggctt ctacggcatc tgcgccgtac
      241 atgcgaacaa acgcattcag atattgcagc ggctggcgat cttcttcctg tgccagtaat
      301 aacaacgttt gcaggcagcg ataatagttg gcgcgttccg gactaaatac tgatgagtta
      361 aactccatcg tccattcggt ccagaccaga gcctgttcca gatcgccacc agccagcgcc
      421 agcatggctt ttaattcacc gatacgcagg gtgtaccaac cgttatccga cccggtcgcc
      481 agacccaaca gctcacgcac gcgggtaaag tcatcaaaac cttcttcatc cagttgctcg
      541 atgaggttca ggtaatcttc tttttcccac tcgctgcctg gtagcgaaag aatcgtttca
      601 cgtaaatggc tgcccatact gttattcgcg agccacagat cttcagccgg ataaatatcg
      661 gacatgccag gcacgataat gcggcaagcg tagacaccca gatgctcgta atcggcaata
      721 taaacttctt tatcttcctt tttgaagata gccatcagcg tggcgaactc ttcttccgtg
      781 gtgccggaga aattccagtc cacaaacgga taatcggcat cctgcttgaa caggtcccag
      841 gagattaaac cgctggaatc gataaagtgc gtttcgaggt tggtatgttc agcgacttct
      901 tcatcatcga aggttggcgg agtaaacaca tccaaatctt tcaggccacg accttgcagc
      961 agctcggtca cggtacgttc cagtgctacg ccaaaatcag gatgcgcacc gaaagaggca
     1021 aagcaggtac cgttagcagg attgaacagt accacgcaaa tcaccggata ctggccgcca
     1081 agcgaaccgt cataagcgaa gattgggaaa ccctccgctt ccagcgtttc gatggcttca
     1141 actactgctg ggtaacgcgc cagcacgtct gccgggatct ctggcaggct gatgctttca
     1201 gcaataatgc ggtttttcac gtagcgttcg aaaacttcag acaatccctg aacgcgtgct
     1261 tcgttgcggg tattacctgc ggacataccg ttggagacgt acaggttacc aatgatattc
     1321 atcggaatat aaacggtctg attgtcggac tgacgcgtaa acggcaggcc gcaaatacca
     1381 cgatcttcgt taccggattg taggtcaatc agcatgctgc cggtcagttc attctccgga
     1441 tcataaaacg cgcgcagacg gtcatcgagt agtccttctg gcacatcgtc attttcggtc
     1501 agtgggaacc atttttcgtt gggataatgc acgaacggac cgttggcgat ggtttcgccc
     1561 agccagaaat cagcaaaaaa gtagttggtt gagagacgct cgaaatattc accaagtgca
     1621 gaagccagcg ccgctttttt ggttgcgcct ttaccgttgg taaaacacag tgcgcactct
     1681 ttgtcgcgaa tatgtacaga ccagacgtta ggcacaggat tcagccagga ggcctcttca
     1741 atctgaaagc cgaggtctga aagtttttgc tggaagcgag cgatggaatc ttccagagcg
     1801 gcatctttgc cggggataaa tgtttgcgtc atgaaaatca ctttagtcgt acggaaagcg
     1861 cgcaataata cgggttttat ctcaaaggcg ctatcaccgc cgccatgccg atgaactgtt
     1921 gactatgctt ttagcggata aaccacgata agagcataaa aatgaaggcg ttcgatctcc
     1981 accgtatggc atttgataaa gtgccttttg atttccttgg cgaagttgca ctacgtagtc
     2041 tttatacctt tgtactggtt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac
     2101 agatgtcgct gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg
     2161 cgttttatga tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt
     2221 tataccgcct ggtaatgtgg ttgatggcgc acagtgaaaa actggaagat ttactggaag
     2281 gtaagccggt tgttattatt gaagatggcg agctggcctg gtcgaaactc aataactcca
     2341 acatgacgga atttgagttc tttatggagc tacgattgtg tggcgtggag cagctggggc
     2401 aggtacgtct ggcgattctc gaaaccaacg ggcaaatcag tgtctatttc tttgaagatg
     2461 acaaggtgaa accgggttta cttattttac ccagtgattg tacgcagcgt tacaaagtgg
     2521 tgccggagtc ggcggactat gcctgtattc gttgtagtga aatcattcat atgaaagcgg
     2581 gggaaaaaca attatgtccg cgctgtgcaa atccagaatg gacgaaggca agtcgggcaa
     2641 aacgggtgac ctgacagtaa aaacatcggc tttttgctaa taatccgaga gattcttttg
     2701 tgtgatgcaa gccacatttt tgccctcaac ggttttactc attgcgatgt gtgtcactga
     2761 atgataaaac cgatagccac aggaataatg tattacctgt ggtcgcaatc gattgaccgc
     2821 gggttaatag caacgcaacg tggtgagggg aaatggctca aatcttcaat tttagttctg
     2881 gtccggcaat gctaccggca gaggtgctta aacaggctca acaggaactg cgcgactgga
     2941 acggtcttgg tacgtcggtg atggaagtga gtcaccgtgg caaagagttc attcaggttg
     3001 cagaggaagc cgagaaggat tttcgcgatc ttcttaatgt cccctccaac tacaaggtat
     3061 tattctgcca tggcggtggt cgcggtcagt ttgctgcggt accgctgaat attctcggtg
     3121 ataaaaccac cgcagattat gttgatgccg gttactgggc ggcaagtgcc attaaagaag
     3181 cgaaaaaata ctgcacgcct aatgtctttg acgccaaagt gactgttgat ggtctgcgcg
     3241 cggttaagcc aatgcgtgaa tggcaactct ctgataatgc tgcttatatg cattactgcc
     3301 cgaatgaaac catcgacggt atcgccatcg acgaaacgcc agacttcggc aaagatgtgg
     3361 tggtcgccgc cgacttctct tcaaccattc tttcccgtcc gattgacgtc agccgttatg
     3421 gcgtgattta cgctggcgcg cagaaaaata tcggcccggc tggcctgaca atcgtcatcg
     3481 ttcgtgaaga tttgctgggc aaagcgaata tcgcgtgtcc gtcgattctg gattattcca
     3541 tcctcaacga taacggctcc atgtttaaca cgccgccgac atttgcctgg tatctatctg
     3601 gtctggtctt taaatggctg aaagcgaacg gcggtgtagc tgaaatggat aaaatcaatc
     3661 agcaaaaagc agaactgcta tatggggtga ttgataacag cgatttctac cgcaatgacg
     3721 tggcgaaagc taaccgttcg cggatgaacg tgccgttcca gttggcggac agtgcgcttg
     3781 acaaattgtt ccttgaagag tcttttgctg ctggccttca tgcactgaaa ggtcaccgtg
     3841 tggtcggcgg aatgcgcgct tctatttata acgccatgcc gctggaaggc gttaaagcgc
     3901 tgacagactt catggttgag ttcgaacgcc gtcacggtta atgccgaaat tttgcttaat
     3961 ccccacagcc agcctgtggg gtttttattt ctgttgtaga gagttgagtt catggaatcc
     4021 ctgacgttac aacccatcgc tcgtgtcgat ggcactatta atctgcccgg ttccaagagc
     4081 gtttctaacc gcgctttatt gctggcggca ttagcacacg gcaaaacagt attaaccaat
     4141 ctgctggata gcgatgacgt gcgccatatg ctgaatgcat taacagcgtt aggggtaagc
     4201 tatacgcttt cagccgatcg tacgcgttgc gaaattatcg gtaacggcgg tccattacac
     4261 gcagaaggtg ccctggagtt gttcctcggt aacgccggaa cggcaatgcg tccgctggcg
     4321 gcagctcttt gtctgggtag caatgatatt gtgctgaccg gtgagccgcg tatgaaagaa
     4381 cgcccgattg gtcatctggt ggatgcgctg cgcctgggcg gggcgaagat cacttacctg
     4441 gaacaagaaa attatccgcc gttgcgttta cagggcggct ttactggcgg caacgttgac
     4501 gttgatggct ctgtttccag ccaattcctc accgcactgt taatgactgc gcctcttgcg
     4561 ccggaagata cggtgattcg tattaaaggc gatctggttt ctaaacctta tatcgacatc
     4621 acactcaatc tgatgaagac gtttggtgtt gaaattgaaa atcagcacta tcaacaattt
     4681 gtcgtaaaag gcgggcagtc ttatcagtct ccgggtactt atttggtcga aggcgatgca
     4741 tcttcggctt cttactttct ggcagcagca gcaatcaaag gcggcactgt aaaagtgacc
     4801 ggtattggac gtaacagtat gcagggtgat attcgctttg ctgatgtgct ggaaaaaatg
     4861 ggcgcgacca tttgctgggg cgatgattat atttcctgca cgcgtggtga actgaacgct
     4921 attgatatgg atatgaacca tattcccgat gcggcgatga ccattgccac ggcggcgtta
     4981 tttgcaaaag gcaccaccac gctgcgcaat atctataact ggcgtgttaa agaaaccgat
     5041 cgcctgtttg cgatggcaac agaactgcgt aaagtcggtg cggaagtaga agaggggcac
     5101 gattacattc gtatcactcc accggaaaaa ctgaactttg ccgagatcgc gacatacaat
     5161 gatcaccgga tggcgatgtg tttctcgctg gtggcgttgt cagatacacc agtgacgatt
     5221 cttgatccca aatgtacggc caaaacattt ccggattatt tcgagcagtt ggcgcggatt
     5281 agccaggcag cctgaatgaa caacgggcaa taaatagcca aatctttctt tatcaaaacg
     5341 tcggcacatt gtcggcgttt tttttcggac cttgtgagtc attttgatta atggtagcgt
     5401 cgcttgtcaa tgtaagttgt tgatacataa tatttatata tgattaatca acggatgatt
     5461 cacatgaaga atactaaatt actgctggcg attgcgacct ctgcagcatt actgacaggg
     5521 tgtcaaaata cccacggtat tgataccaat atggctatca gctccggttt aaatgcctat
     5581 aaagcagcaa cattaagcga tgccgatgca aaagcgattg ccaatcaggg ctgtgccgaa
     5641 atggacagcg gcaatcaagt cgcaagtaaa tccagcaagt acggtaaacg tctggcaaaa
     5701 atcgccaaag cattgggtaa caatattaat ggcacgccgg tcaactataa ggtttatatg
     5761 accagcgacg tcaacgcatg ggcgatggcg aacggctgtg tccgtgtcta cagtggcctg
     5821 atggacatga tgaatgacaa cgaaattgaa ggtgttctgg gccatgaact gggccacgtc
     5881 gcgttgggtc actcgctggc tgaaatgaaa gcttcttatg cgatcgttgc cgcacgcgat
     5941 gccatttcag ctaccagcgg agtggcttcc cagctttccc gctcacaatt gggtgatatc
     6001 gcagaaggcg ctattaatgc taaatactcc cgtgataaag agtccgaagc agatgatttc
     6061 tcctttgatc tgttgaagaa acgtggcatc agcacccagg ggctggttgg cagctttgaa
     6121 aaactggcta gtctggatgg cggtcgcacc cagtccatgt ttgactctca cccaccatca
     6181 acagagcgtg cgcaacacat ccgtgatcgt atcgcctctg gtaagtaaat cattgtcatc
     6241 tttcgggctg gtcttctgcc agcccgctat aattgcgcaa taaatcccca tctgaataca
     6301 gacaaaactg gtttttgcac acaacgttaa cgatttgtgg cgtcggcgcg tataatgcgc
     6361 gcggttatgt taacggtacg cctgttttaa ggagataaag atgacggcaa ttgccccggt
     6421 tattaccatt gatggcccaa gcggtgcagg gaaaggcacc ttgtgtaagg ctatggcgga
     6481 agcattgcaa tggcatctgc tggattcggg tgcaatttat cgcgtactgg cattggcggc
     6541 attacatcac catgttgatg ttgcgtcaga agatgcgctg gtaccgctgg catcccatct
     6601 ggatgtacgt tttgtgtcaa ccaatggcaa tctggaagtg atcctcgaag gggaagatgt
     6661 cagcggcgaa attcgtactc aggaagtggc gaatgcagct tcacaagtcg cggcattccc
     6721 acgcgttcgt gaagcattat tgcgtcgcca acgcgcgttt cgcgaattac caggcctgat
     6781 tgccgatggc cgcgacatgg gaacggtggt attccctgat gcgccagtga aaattttcct
     6841 tgacgcctcc tcggaagaac gtgcgcatcg ccgcatgcta cagttgcagg agaagggctt
     6901 tagtgttaac tttgagcgcc ttttggccga gatcaaagaa cgcgacgacc gcgatcgtaa
     6961 ccgagcggta gcgccactgg ttccggcagc cgatgcttta gtgttggatt ccaccacctt
     7021 aagcattgag caagtgattg aaaaagcgct acaatacgcg cgccagaaat tggctctcgc
     7081 ataagcgacc gaatttgcag tacccccgtt gcaatggaat gacagcgggt atgttaaaca
     7141 accccatccg gcatggagcc aggtggacgt taaatataaa cctgaagatt aaacatgact
     7201 gaatcttttg ctcaactctt tgaagagtcc ttaaaagaaa tcgaaacccg cccgggttct
     7261 atcgttcgtg gcgttgttgt tgctatcgac aaagacgtag tactggttga cgctggtctg
     7321 aaatctgagt ccgccatccc ggctgaacag ttcaaaaacg cccagggcga gctggaaatc
     7381 caggtaggtg acgaagttga cgttgctctg gacgcagtag aagacggctt cggtgaaact
     7441 ctgctgtccc gtgagaaagc taaacgtcac gaagcctgga tcacgctgga aaaagcttac
     7501 gaagatgctg aaactgttac cggtgttatc aacggcaaag ttaagggcgg cttcactgtt
     7561 gagctgaacg gtattcgtgc gttcctgcca ggttctctgg tagacgttcg tccggtgcgt
     7621 gacactctgc acctggaagg caaagagctt gaatttaaag taatcaagct ggatcagaag
     7681 cgcaacaacg ttgttgtttc tcgtcgtgcc gttatcgaat ccgaaaacag cgcagagcgc
     7741 gatcagctgc tggaaaacct gcaggaaggc atggaagtta aaggtatcgt taagaacctc
     7801 actgactacg gtgcattcgt tgatctgggc ggcgttgacg gcctgctgca catcactgac
     7861 atggcctgga aacgcgttaa gcatccgagc gaaatcgtca acgtgggcga cgaaatcact
     7921 gttaaagtgc tgaagttcga ccgcgaacgt acccgtgtat ccctgggcct gaaacagttg
     7981 ggcgaagatc cgtgggtagc tatcgctaaa cgttatccgg aaggtaccaa actgactggt
     8041 cgcgtgacca acctgaccga ctacggctgc ttcgttgaaa tcgaagaagg cgttgaaggc
     8101 ctggtacacg tttccgaaat ggattggacc aacaaaaaca tccacccgtc caaagttgtt
     8161 aacgttggcg atgtagtgga agttatggtt ctggatatcg acgaagaacg tcgtcgtatc
     8221 tccctgggtc tgaaacagtg caaagctaac ccgtggcagc agttcgcgga aacccacaac
     8281 aagggcgacc gtgttgaagg taaaatcaag tctatcactg acttcggtat cttcatcggc
     8341 ctggacggcg gcatcgacgg cctggttcac ctgtctgaca tctcctggaa cgttgcaggc
     8401 gaagaagcag ttcgtgaata caaaaaaggc gacgaaatcg ctgcagttgt tctgcaggtt
     8461 gacgcagaac gtgaacgtat ctccctgggc gttaaacagc tcgcagaaga tccgttcaac
     8521 aactgggttg ctctgaacaa gaaaggcgct atcgtaaccg gtaaagtaac tgcagttgac
     8581 gctaaaggcg caaccgtaga actggctgac ggcgttgaag gttacctgcg tgcttctgaa
     8641 gcatcccgtg accgcgttga agacgctacc ctggttctga gcgttggcga cgaagttgaa
     8701 gctaaattca ccggcgttga tcgtaaaaac cgcgcaatca gcctgtctgt tcgtgcgaaa
     8761 gacgaagctg acgagaaaga tgcaatcgca actgttaaca aacaggaaga tgcaaacttc
     8821 tccaacaacg caatggctga agctttcaaa gcagctaaag gcgagtaatt ctctgactct
     8881 tcgggatttt tattccgaag tttgttgagt ttacttgaca gattgcaggt ttcgtcctgt
     8941 aatcaagcac taagggcggc tacggccgcc cttaatcaat gcagcaacag cagccgctta
     9001 atttgccttt aaggaaccgg aggaatcatg accaagtcag aattgataga aagacttgcc
     9061 acccagcaat cgcacattcc cgccaagacg gttgaagatg cagtaaaaga gatgctggag
     9121 catatggcct cgactcttgc gcagggcgag cgtattgaaa tccgcggttt cggcagtttc
     9181 tctttgcact accgcgcacc acgtaccgga cgtaatccga agactggcga taaagtagaa
     9241 ctggaaggaa aatacgttcc tcactttaaa cctggtaaag aactgcgcga tcgcgccaat
     9301 atttacggtt aagtttttac tcaaacttga acgcgagaaa agcacctgtc gggtgctttt
     9361 ttcatttctc taatcaggaa ctggaagctg cctcgcagag ttttgagcag ttttcaccct
     9421 ttcgttaaat tcttctgaat gtgcctcggg gaacgcaaaa ttcccaccag acaaccgctc
     9481 aacaaagttg cacactttcc ataaacaggg aggggtgcat gaaaataacg acagtcggcg
     9541 tatgcataat ttgcggaatt tttccgttgc tgattttgcc ccaattgcct gggacattaa
     9601 cccttgcgtt tctgactctc ttcgcctgcg tactggcatt tatccctgtt aaaaccgtcc
     9661 gttatatcgc gctgacgttg ctgtttttcg tttggggcat attatcagca aagcaaattt
     9721 tgtgggcagg agaaacctta actggcgcga cgcaggatgc aattgttgag atcactgcaa
     9781 ctgacggcat gaccactcat tacggtcaaa ttactcatct acaaggtcga cgtatattcc
     9841 ctgcgccagg ccttgtgctg tatggcgaat atcttccgca agcggtttgt gctggacaac
     9901 aatggtcaat gaaactcaaa gttcgtgcgg ttcatggcca acttaatgat ggcggctttg
     9961 atagccagcg ttatgccatt gcccagcatc aaccgctcac cggccgcttt ctgcaggcaa
    10021 gtgttattga accgaattgt agcctgcgtg cacagtatct ggcgtcacta caaacaacgc
    10081 tgcaacccta tccgtggaat gcggttattc ttggtttagg tatgggggaa cggttatccg
    10141 tccccaaaga aatcaaaaat atcatgcgcg atactggaac ggcgcattta atggcgatat
    10201 cgggattgca catcgctttt gcggcgttgc tggctgccgg actcattcgc agtggacaaa
    10261 tttttctgcc tgggcgctgg atccactggc aaataccatt aattggtggg atctgctgtg
    10321 ctgcttttta tgcctggttg acgggaatgc aacctcctgc attgcgtacc gtggtggcgc
    10381 ttgctacgtg gggaatgctt aagttaagtg ggcgacagtg gagtggctgg gatgtatgga
    10441 tatgttgtct ggcggcaatt ttgctgatgg atcctgttgc cattctctcg caaagtttat
    10501 ggctctctgc cgctgcggtc gcggcactga ttttttggta tcagtggttt ccctgtcctg
    10561 agtggcaact gccgccggta ttgcgtgcag ttgtttccct catccatctg caactgggta
    10621 tcacacttct gcttatgccc gtgcaaatcg tcatatttca tggcattagt ctgacctcgt
    10681 ttattgcaaa tctattagca attcccttgg tgacatttat cacggttccg ttgatcctcg
    10741 ccgcgatggt tgtgcattta agcgggccgt taatcctgga gcaagggtta tggtttcttg
    10801 ccgaccggtc tttggcttta cttttctggg ggttaaagag tttgccagaa gggtggatca
    10861 acattgctga acgttggcaa tggctatcat tttccccatg gttcttactg gtggtatggc
    10921 gattaaacgc ctggcgaacg ttgccagcaa tgtgtgtggc tgtaggcttg ctgatgtgct
    10981 ggccgctatg gcaaaaacct cgacctgacg agtggcaggt gtacatgctt gatgtcgggc
    11041 aagggctggc aatggtgata gccagaaacg gcaaagcgat tctctatgac acaggactgg
    11101 cctggcccga aggggatagt gggcaacaac tgattatccc ctggctccac tggcataatc
    11161 ttgaaccgga aggcgttatt ctgagtcatg aacatctgga tcaccgggga gggctggact
    11221 caatattgca cacatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc
    11281 agccctgtgt gcgtggcgaa gcgtggcaat ggcaaggatt gcgtttcagc gtgcactggc
    11341 ctttacaagc tagcaacgat aaaggaaata accattcctg tgtggttaag gttgatgacg
    11401 ggacgaatag cattcttcta accggtgata ttgaagtccc cgctgaacaa aagatgctaa
    11461 gccgttactg gcagcaagtg cagacaacat tgcttcaggt acctcactat ggcagtaata
    11521 cctcatcatc gttgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat
    11581 cgcgctataa cgcatggcga ttgccctcta ataaagttaa gcatcgctat caacagcaag
    11641 gatatcaatg gcttgatact ccacatcagg gtcaagtgac ggtcaatttt tcagcgcaag
    11701 gctggcggat tagcagcctc agagagcaaa ttttacctcg ttggtatcat cagtggtttg
    11761 gcgtgccagt ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa
    11821 tgcataacga caaagatctc tctacgtggc agacgttccg ccgactgtgg ccaaccattg
    11881 cgccttttaa agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg
    11941 ataccttcat gttatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc
    12001 gctccgtgct tgtgtggatg ccgctggtgg tgatcgggct gatgatttta cgtggtatca
    12061 ccagctatgt ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc
    12121 gtcgccgcct gtttggtcac atgatgggaa tgccggtttc attttttgac aaacagtcaa
    12181 cggggacgct gttgtcacgt atcacctacg attccgaaca ggttgcttct tcttcttccg
    12241 gcgcactgat aacggttgtg cgtgaaggtg cgtcaatcat cggcctgttc atcatgatgt
    12301 tctattacag ttggcaactg tcgatcattt tgattgtgct ggcaccgatt gtttcgattg
    12361 cgattcgcgt agtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg
    12421 ggcaggtgac caccagcgcc gaacaaatgc tgaagggcca caaagaagta ttgattttcg
    12481 gtggtcagga agtggaaacg aaacgctttg ataaagtcag caaccgaatg cgtcttcagg
    12541 ggatgaaaat ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt
    12601 tggcgctggc gtttgttctg tatgcggcga gcttcccaag tgtcatggat agcctgactg
    12661 ccggtacgat taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcac
    12721 tgaccaacgt taacgcccag ttccagcgcg gtatggcggc ttgtcagacg ctgtttacca
    12781 ttctggacag tgagcaggag aaagacgaag gtaagcgcgt gatcgagcgt gcgactggcg
    12841 acgtggaatt ccgcaatgtc acctttactt atccgggacg tgacgtacct gcattgcgta
    12901 acatcaacct gaaaattccg gcagggaaga cggttgctct ggttggacgc tctggttcag
    12961 gtaaatcaac catcgccagc ctgatcactc gtttttacga tattgatgaa ggcgaaatcc
    13021 tgatggatgg tcacgatctg cgcgagtata ccctggcgtc gttacgtaac caggttgctc
    13081 tggtgtcgca gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac
    13141 ggactgaaca gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg
    13201 acttcatcaa taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtgctgc
    13261 tctctggcgg tcagcgtcag cgtattgcta tcgctcgagc cttgctgcgt gatagcccga
    13321 ttctgattct ggatgaagct acctcggctc tggataccga atccgaacgt gcgattcagg
    13381 cggcactgga tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta
    13441 ccattgaaaa ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta
    13501 cgcataacga tttgcttgag caccgtggcg tttacgcgca acttcacaaa atgcagtttg
    13561 gccaatgatc gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact
    13621 ctcctggttg tatggcctgg taagtggcgc gatccgtctt tgctataaac taaaactgaa
    13681 gcgcgcctgg cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa
    13741 cggaaaaacc ccggtcgttg tctggctggt ggaacagttg caacagcgcg gtattcgcgt
    13801 gggggtcgta tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc
    13861 agataccaca acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc
    13921 gcctgttgcg gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc
    13981 tgatgtgcag atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt
    14041 ggaaattgtc gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg
    14101 gccaatgcgt gagcgagcgg ggcgcttaaa gtcggttgat gcggtaatcg tcaacggcgg
    14161 tgtccctcgc agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg
    14221 taccggtacg cgttgtgacg ttgctcagct tgaacatgta gtggcgatgg cggggattgg
    14281 gcatccgccg cgcttttttg ccacgctgaa gatgtgtggc gtacaaccgg aaaaatgtgt
    14341 accgctggcc gatcatcagt ctttgaacca tgcggatgtc agtgcgttgg taagcgccgg
    14401 gcaaacgctg gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa
    14461 ttggtggtat ttgcctgtag acgcacagct ttcaggtgat gaaccagcga aactgcttac
    14521 gcaactaacc tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga
    14581 gtcatgaatg tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc
    14641 acaaggcctg ttaaacaaac cccgccgtca agcgtcgttg gaggatattc cggcaacgat
    14701 ctcccgcatg tccttgctgc aaatcgatac catcaatatt gttgcccgta gtccatatct
    14761 ggtgcttttc agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg
    14821 tggcgaatta atggaatact gggcgcatga agcctgcttt atgccgcgta gcgactttcg
    14881 tcttattcgc caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg
    14941 gatgcaggaa catgaggcgg aaattgcaca gttaattcag catattcatg ataaggggcc
    15001 ggtacgttca gccgattttg agcatcctcg taaaggtgca agcggctggt gggaatggaa
    15061 gccgcataaa cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg
    15121 gcgcaacttc cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga
    15181 gcgcgatctc gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag
    15241 cctgggaata ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact
    15301 ggcggcgtgg cgcgaagcga gggctgaaca gcagcaaatc attgctgtgc atgttgaaaa
    15361 attgggcaat ctttggctgc atgctgattt gctgccgcta ctcgagcgtg cgctggccgg
    15421 aaagctcact gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg
    15481 caaacgcgca gagcagcttt ttgattttag ctacctgctg gagtgctata ccccagcgcc
    15541 gaaacgccag tatggctatt ttgttctgcc attattacat cgtgggcaat tagttgggcg
    15601 aatggatgcc aaaatgcatc gccagacagg catccttgaa gttatctctc tgtggttaca
    15661 ggaaggcatt aaaccaacga caatgctgca aaaagggtta cgtcaggcga ttactgattt
    15721 cgcgagctgg cagcaggcaa cgcgggtgac attaggacgc tgcccgcaag gtctctttac
    15781 tgattgccgc actggctggg aaatagaccc cgtcgcataa atgattatga taagatttat
    15841 gcattcataa cccgatccat ctggaggaac tatggatcat cgtctgcttg aaatcattgc
    15901 ctgcccggtt tgcaacggaa aactttgcta taaccaagaa aaacaagaac ttatttgcaa
    15961 actggacaac ctggctttcc cgctgcgtga tggcattcca gtgttgctgg aaacggaagc
    16021 ccgcgtgctg actgctgatg agagtaaatc atgagttttg tggtcattat tcccgcgcgc
    16081 tacgcgtcaa cgcgtctgcc cggtaaacca ttggttgata ttaatggcaa acccatgatt
    16141 gttcatgttc ttgaacgtgc tcgcgaatca ggtgccgatc gcatcatcgt ggcaaccgat
    16201 catgaggatg tcgcccgcgc cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc
    16261 gatcaccagt cagggacaga acgcctggcg gaagttgtcg aaaaatgcgc attcagcgac
    16321 gacacggtga tcgttaatgt gcagggtgat gaaccgatga tccctgcgac aatcattcgt
    16381 caggttgctg ataacctcgc tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc
    16441 cacaatgcgg aagaagcgtt taacccgaat gcggtgaaag tggttctcga cgctgaaggg
    16501 tatgcactgt acttctctcg cgccaccatt ccttgggatc gtgatcgttt tgcagaaggc
    16561 cttgaaaccg ttggcgataa cttcctgcgt catcttggta tttatggcta ccgtgcaggc
    16621 tttatccgtc gttacgtcaa ctggcagcca agtccgttag aacacatcga aatgttagag
    16681 cagcttcgtg ttctgtggta cggcgaaaaa atccatgttg ctgttgctca ggaagttcct
    16741 ggcacaggtg tggatacccc tgaagatctt gagcgcgttc gcgctgaaat gcgctaattt
    16801 cacttcacga cacttcagcc aattttggga ggagtgtcgt accgttacga ttttcctcaa
    16861 tttttctttt caacaattga tctcattcag gtgacatctt ttatattggc gctcattatg
    16921 aaagcagtag cttttatgag ggtaatctga atggaacagc tgcgtgccga attaagccat
    16981 ttactgggcg aaaaactcag tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg
    17041 tgggctttgt atgacagcca gggaaaccca atgccgttaa tggcaagaag ctttagtacg
    17101 cccggaaaag cccgacaact ggcatggaaa accaccatgc tggcaagaag tgggactgtc
    17161 cgtatgccga ctatttatgg tgtgatgacg catgaagaac accccggccc cgatgtcctg
    17221 ctactggagc ggatgcgtgg tgtttcggtg gaggcaccag cccgaacacc agaacgctgg
    17281 gaacaactca aagaccaaat cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc
    17341 ggttgcgtcg gcgcggtcga caatactcag gaaaatttct ggccctcatg gtaccggcaa
    17401 catgttgaag tgctatggac cacgctcaat cagttcaata acaccggtct gacgatgcag
    17461 gataagcgga tcctgtttcg cactcgcgaa tgtctcccgg cattatttga aggctttaac
    17521 gacaattgtg tgctgattca cggtaacttc tgtttacgca gcatgttgaa agattcgcgc
    17581 agcgatcagt tactggcgat ggtcgggccg ggactaatgc tttgggcacc gcgagaatac
    17641 gaactgttcc gactaatgga taattctctg gcggaagatt tgctctggag ttacctgcaa
    17701 cgcgcgccag tggcggagtc gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa
    17761 gttgcgcaac tggttaatac cggacgattt agtcggcgca acttcgatct ggcatcaaaa
    17821 tcactcttgc cgtggctcgc ctgacgaacc ttttagccac tgccagatac ggccaagcgt
    17881 ttcatagcca acacgatcgc tatgcatcaa ccagaccgga gaagggattg cccgttccca
    17941 cgggttaagc ggcgagtcga tggccaactg attcgccggg gctggtagcg gatttaaccc
    18001 ttcctgctga aaaaagatca tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa
    18061 aggggcgtca ccaatcgcct gtttcactgc cgcagcttct tcttcggtat cttttggcaa
    18121 atccagggtg ataatttgct cgtgcggcac gcccagcgat tgcgcaactc tggcacctac
    18181 ttccgctgta ctcaccgtat tggtttttgc tacgcctccc gtgaagatca gttttgatcc
    18241 cggattttcg cgccataagc gaataccttc attcaggcgt ggcagactgt tattgattaa
    18301 attagagctc ggtgcccact gtgggttcca ggtataacca cctccgagca ccacgatata
    18361 gtccactttt tgcgaattat tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg
    18421 gtcggctacc ggttgcaaac tcaataacaa gagtgccagc cacccaatgc taataaaaat
    18481 cttcccggtt ttttgaaaac ggctaaacca cagcagcgcc aggccagcgc caataattaa
    18541 caacatcagc ggaagtggca gcaacatatt gccaatcact tttttcagtg taaaaagcat
    18601 cctttctggt tcctttttta accatatagc aagggatcgc cagcgatatt acaccagacg
    18661 ggttcattct cgccgtggct gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa
    18721 ccggtggaga aagcaatgca ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt
    18781 aacatttacg gcaccaccaa agggcagctt cgacaggcta ttctgtggca ggatctcgat
    18841 cacgtgctgg cggaaatggg cccgcaaaaa ctgcgtgtgc tggatgctgg cggtggtgaa
    18901 gggcagaccg caatcaaaat ggccgagcgt gggcatcagg tcattttatg cgatctttct
    18961 gcgcagatga tcgaccgcgc aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg
    19021 caatttatac attgcgccgc tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg
    19081 atattgttcc atgcggtgct cgagtgggtg gccgatcccc gcagtgtatt gcagaccctg
    19141 tggtcagtgt tgcgtccagg cggagtgttg tcgttaatgt tctacaatgc gcatggtttg
    19201 ttgatgcata acatggtcgc cgggaatttt gattacgtgc aggcgggaat gccgaaaaag
    19261 aaaaaacgga cgctttcgcc agattatcca cgcgacccgg cgcaggttta tctgtggctg
    19321 gaagaagctg gttggcaaat tatgggtaag acaggcgttc gcgtgtttca tgattatctg
    19381 cgcgagaagc accagcagcg cgactgctat gaagcattac ttgaattaga aacgcgttat
    19441 tgccgtcagg aaccgtatat taccctgggg cgttatattc atgtcaccgc gcgcaaaccg
    19501 cagagcaagg ataaagtatg agtgaatttt cccagacagt ccccgaactg gttgcctggg
    19561 ccagaaaaaa tgacttctcc atctcgctgc cggtagaccg actctctttt ctgctggcgg
    19621 ttgccacgct gaacggcgag cgtctggatg gtgagatgag tgaaggcgag ctggtggatg
    19681 cattccgcca tgtgagtgat gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca
    19741 acaacgcgat caacgacatg gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg
    19801 cggaagggaa cgcaatttac cgtctgacgc cgctcggcat cggcatcact gactattaca
    19861 ttcgtcagcg cgagttttct acgctgcgcc tttctatgca gttgtcgatt gtggcgggtg
    19921 agctcaaacg cgcagcagat gccgccgaag agggcggtga tgaatttcac tggcaccgta
    19981 atgtttatgc tccactgaaa tattcggtag cagaaatttt cgacagtatc gacctgacgc
    20041 aacgtctgat ggatgaacag cagcagcagg tgaaggatga tatcgcccag ttgctgaaca
    20101 aagactggcg ggcggcgatt tccagctgtg aattgttgct ttcggaaact tccggaacgc
    20161 tgcgtgaatt gcaggatacg ctggaagcgg caggcgacaa attgcaggct aatctgttgc
    20221 gcattcagga tgcgacgatg acccatgacg atctgcattt cgtcgatcgt ctggtgttcg
    20281 atctgcagag caaactcgat cgtattatca gttggggcca gcaatccatc gacttgtgga
    20341 ttggctacga ccgccacgta cacaaattta ttcgtaccgc gatcgatatg gataaaaacc
    20401 gcgtctttgc tcagcggtta cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc
    20461 taacttatgc caatgccgat cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg
    20521 atgaagaagt gactggggaa cttcctgagg atctggaata cgaagagttt aacgagatcc
    20581 gcgaacagct ggcggcgatc atcgaagaac aacttgccgt gtacaaaacc agacaagtgc
    20641 cgctggatct tggtctggtg gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact
    20701 ttgacgttgc gcgcattgtt attgatcagg cggtacgtct tggcgtagcg caagcagatt
    20761 tcaccggact gccagcgaaa tggcagccga ttaatgatta cggagccaag gtacaggcgc
    20821 atgtcatcga caaatattga acaagtgatg ccggttaagc tggcgcaggc gctggcgaat
    20881 ccgttatttc cggcgctgga cagcgcctta cgttcaggcc gccatattgg cctcgacgaa
    20941 ctggataatc atgcattcct gatggatttt caggaatatc tggaagagtt ttacgcgcgt
    21001 tataacgttg agcttattcg cgcaccggaa gggttcttct atttacgccc acgttccacc
    21061 actcttatcc cgcgttccgt cttgtcggaa ctggatatga tggtcgggaa aatcctctgt
    21121 tatctctatc tcagcccgga acggctggcg aatgagggga ttttcaccca gcaggaactg
    21181 tacgacgaac tgctcaccct ggccgatgaa gcaaaactgc tgaaactggt gaacaaccgt
    21241 tcaaccggtt cagacgttga ccgtcagaag ttgcaggaga aagtacgttc ttcgctcaac
    21301 cgtctgcgtc gtttaggcat ggtgtggttt atgggccacg acagcagcaa gtttcgcatt
    21361 accgaatcgg tgttccgctt cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca
    21421 cagcgtcgcc tgattcgtga tggcgaagca atgccgattg aaaatcatct gcaactcaac
    21481 gatgaaaccg aagagaatca gccagatagc ggagaggaag aataatgatt gaacgcggta
    21541 aatttcgctc actgacgctg attaactgga acggcttttt tgcccgaact tttgaccttg
    21601 acgagctggt cacgacgctt tctggcggta acggggcggg taaatccacc accatggcgg
    21661 cgttcgttac ggcgctgatc cccgacctga ccctgctgca tttccgtaac actacggaag
    21721 ccggggccac cagcggttcg cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct
    21781 gttattcgat gctcgacacc attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc
    21841 tgcaacaggt tgccggacgc gatcgtaaag tggatatcaa accatttgcc attcagggac
    21901 tgccgatgtc ggtgcagccg acacagctgg tgaccgaaac cctgaacgaa cgccaggcgc
    21961 gcgtgctgcc gcttaacgag ctgaaagaca agctcgaggc gatggaaggc gtgcagttta
    22021 aacagttcaa ctccattacc gattaccact cgctgatgtt cgatctgggc atcatcgcgc
    22081 gtcgtctgcg ctccgcatct gaccgtagca aattctatcg tctgatcgaa gcttcgctgt
    22141 atggcgggat ctccagtgcc attacccgtt ctctgcgcga ctacctgttg ccggaaaaca
    22201 gcggcgtgcg taaagcgttc caggacatgg aagcggcgct gcgtgaaaac cgcatgacgc
    22261 tggaagcgat tcgtgtcacc cagtcggacc gtgacctgtt taagcatctg atcagcgaag
    22321 ctaccaacta cgtggcggcg gactacatgc gtcacgccaa cgagcgccgt gtccatctcg
    22381 acaaagccct ggagtttcgt cgcgagttac atacttcgcg tcagcaactg gcggctgagc
    22441 agtacaaaca cgtcgatatg gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc
    22501 tggaagcgga ttatcaggcg gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc
    22561 agcaggagaa gatcgaacgc tacgaagcgg atctcgatga gctgcagatc cgtctggaag
    22621 agcaaaacga agtggtggca gaagccatcg aacgccagga agagaacgag gctcgcgcgg
    22681 aagctgccga actggaagtg gacgagctga aaagccagct tgctgactac cagcaggcgc
    22741 tggacgtcca gcaaacgcgc gcgatccagt ataaccaggc gattgctgcg cttaatcgcg
    22801 ccaaagaact gtgccatctg ccggacttaa ccgccgacag cgccgccgaa tggctggaaa
    22861 ccttccaggc gaaagagctg gaagcgactg aaaaaatgct ctctcttgag cagaaaatga
    22921 gcatggcgca aaccgcgcac agccagtttg agcaggctta tcagctggtg gtggcaatca
    22981 acggcccgct ggcgcgtaac gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg
    23041 tcgatcagcg tcacctggca gagcaggttc aaccgttgcg gatgcgctta agtgaactgg
    23101 aacagcgtct gcgcgagcag caagaagccg aacgtctgct ggcagatttc tgcaaacgtc
    23161 agggcaagaa ttttgatatc gccgaactgg aagccctgca tcaggaactg gaagcacgca
    23221 ttgcctctct ttccgatagc gtgtctaacg cccgtgaaga gcgcatggca ctgcgccagg
    23281 agcaggaaca gctgcagtct cgcattcaga gtttgatgca gcgtgcgccg gtttggctgg
    23341 cagcgcaaaa cagtctcaac cagttgagcg aacagtgcgg cgaagagttt acctccagcc
    23401 aggacgtcac cgaatatctg caacagttgc tggagcgtga gcgagaggcg attgttgaac
    23461 gcgatgaagt gggcgcgcgc aaaaacgccg tcgatgaaga gatcgaacgt ttaagccagc
    23521 ctggcggctc tgaagatcag cgtctgaacg cgctggcgga gcgttttggt ggtgtgctgc
    23581 tgtcagaaat ttatgacgac gttagcctgg aagatgcgcc gtacttctca gcgctgtatg
    23641 gcccgtcacg ccacgccatc gtggtgccag atctgtcaca ggtaactgaa cacctggaag
    23701 gcttgaccga ttgcccggaa gatctctatc tgatcgaagg agatccgcag tcattcgatg
    23761 acagcgtgtt cagcgttgat gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc
    23821 agtggcgtta ttcacgtttc ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc
    23881 gtattgaaag cctccatgcc gagcgtgaag tgctttccga acgcttcgcc acgctctcct
    23941 ttgatgtaca gaaaactcag cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc
    24001 tggcggttgc gtttgagtct gacccggaag cagaaatccg tcaactgaac agccgtcgcg
    24061 tcgaactgga gcgggcgttg agtaatcatg aaaatgataa ccagcagcag cgtattcagt
    24121 ttgagcaggc gaaagaaggc gttacggcgc tgaaccgcat tctgccgcgt ctcaacctgt
    24181 tggctgatga cagcctggcg gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc
    24241 aggaagccgc gcgttttgtt cagcagtttg gcaatcaact ggcgaaactg gaaccgatcg
    24301 tttcggtatt gcagagcgac ccggaacagt tcgaacagct aaaagaagat tacgcgtact
    24361 ctcagcagat gcagcgcgat gcccgtcagc aggcgtttgc cctgacggaa gtggtgcagc
    24421 gtcgtgcgca ctttagctat tctgactcgg cagaaatgct gagcggtaat agcgatctca
    24481 acgaaaagct gcgtgaacgt ctggaacagg cggaagcgga gcgtactcgc gctcgtgaag
    24541 cgttgcgtgg tcacgcagcg cagttgagtc agtacaacca ggtgctggct tcgctgaaaa
    24601 gttcttacga caccaaaaaa gagctactca acgatctgca acgtgaattg caggatatcg
    24661 gcgtgcgtgc tgatagcggg gcagaagagc gggcgcgtat tcgccgtgac gagctgcatg
    24721 cgcaactgag caataaccgt tcacgccgca atcaactgga aaaagcgctt accttctgcg
    24781 aagcggagat ggacaacctg acccgcaaac tgcgcaagct ggagcgggat tactttgaga
    24841 tgcgcgagca ggtagtgacc gccaaagcgg gctggtgtgc ggtgatgcgc atggtgaaag
    24901 ataacggcgt tgagcgccgc ttacaccgtc gtgagctggc ttatctctcc gctgatgatt
    24961 tgcgctctat gtcggataag gcattgggtg cgctgcgtct ggcggtggcg gataacgaac
    25021 atctgcgcga cgtgctgcgc atgtcggaag atccgaaacg tccggagcgt aaaattcagt
    25081 tcttcgtggc ggtttatcag catctgcgtg aacgtattcg tcaggatatt attcgtaccg
    25141 atgatccggt ggaagctatc gaacagatgg agatcgaact tagccgtctg accgaagaat
    25201 taacctcccg tgaacagaaa ctggcgatca gttcccgcag cgtggcgaac atcattcgca
    25261 aaaccattca gcgcgagcag aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat
    25321 cgtttggtca ggtgaacagc gtgcgcctca acgtgaacgt gcgtgaaacg cacgccatgc
    25381 tgctggatgt gctctctgaa cagcacgagc agcatcagga tctgtttaac agcaaccgtc
    25441 tgaccttctc ggaagcgttg gcgaaattgt atcagcgtct taatccgcag attgatatgg
    25501 ggcagcgcac gccgcagacc attggcgaag aactgctgga ttaccgcaac tatctggaaa
    25561 tggaagttga ggttaaccgt ggttccgatg gttggttgcg cgcagagtct ggtgcattgt
    25621 cgaccggtga ggcgattggt accggtatgt cgattctggt gatggtggta caaagctggg
    25681 aagatgaatc tcgccgcctg cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg
    25741 atgaagcagc gcgactggat gctcgttcta tcgccacgct gtttgaattg tgtgagcgtt
    25801 tgcaaatgca gctcatcatc gcagcgccgg aaaatatcag cccggagaaa ggcaccacct
    25861 ataaactggt gcgtaaagtc ttccagaata ccgaacatgt tcatgtcgtc ggcctgcgag
    25921 gatttgcgcc gcaactccct gaaacgcttc caggaactga cgaagcgcct tctcaggcga
    25981 gttaaaatta aggcggcagc aatgccgcct tttctttttc cgaaaactcc gtttctgcac
    26041 taaaaaagtg gcacattacg gcgcgctttt ctttaaactt ctttacatta ggttatgtaa
    26101 aaacgtatcg gcgtttatat actgaagata agcctgatga gtaacaggct tgctcgtcat
    26161 actttcgtga gtattggcgt tgtacaggca agtcgtaaaa taacagcctg gctattcaga
    26221 gtatgataaa aacagggggc aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc
    26281 ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg ttcaatgcgc aggccgatga
    26341 gcctgaagta atccctggcg acagcccggt ggctgtcagt gaacagggcg aggcactgcc
    26401 gcaggcgcaa gccacggcaa taatggcggg gatccagcca ttgcctgaag gtgcggcaga
    26461 aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt tacaagccgg tttatcttaa
    26521 ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc
    26581 tgttaaagcc ttccagcaac agctggcaga ggtggcgatt gccggtttcc agccgcagtt
    26641 taataaatgg gtagagttac tgaccgatcc tggtgttaac gggatggcac gcgacgtagt
    26701 gctctctgat gcgatgatgg gctatctcca tttcattgca aatattccgg tcaaaggcac
    26761 tcgctggcta tatagcagta aaccttatgc acttgcaacg ccgccgctct cggtgattaa
    26821 ccaatggcag ctggcgctgg ataaaggtca attgcctacg tttgttgcag gactggcacc
    26881 gcagcatccg caatatgcgg cgatgcatga atcgttactg gccttactca gtgacaccaa
    26941 accgtggccc caactgaccg gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt
    27001 accggcgttg cgcgaaatat tgcaacgcac aggcatgttg gacggggggc cgaaaattac
    27061 tctacctggc gatgacacgc caactgacgc ggtagtcagc ccatccgctg ttactgttga
    27121 aacagcagaa actaagccga tggacaagca aacgacgtct cgtagtaaac ctgcgcctgc
    27181 cgttcgtgcc gcctacgata atgaactggt ggaagccgtt aaacgttttc aggcatggca
    27241 aggattgggg gcagatggtg ctattggccc ggcaacacgt gactggttaa acgtaacgcc
    27301 cgcccagcgt gctggcgtgt tggctctcaa catccagcga ttgcgcttgc tgccaacaga
    27361 gctttctacc gggatcatgg ttaacattcc ggcctattcg ctggtctact atcagaacgg
    27421 caatcaggtg ctggattcgc gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat
    27481 gagcagtgcc cttaacaacg tagtggtaaa cccgccgtgg aacgtacctc caactctggc
    27541 acgcaaagat attctgccaa aagtgcgcaa cgatccggga tatctcgaaa gccatggcta
    27601 tacggtgatg cgcggctgga acagcagaga agcgattgac ccatggcagg ttgactggtc
    27661 tacaatcacg gcctcgaatt taccgttccg cttccagcag gctccaggcc cacggaactc
    27721 gctggggcgc tataaattca atatgccgag ttcagaggcc atttatttgc atgacacgcc
    27781 gaaccacaat ctgttcaagc gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa
    27841 taaagcttcc gatctggcga atatgctgtt gcaggatgca ggctggaatg acaaacgtat
    27901 ttctgatgcg ctgaagcagg gtgatacacg ttacgtcaat attcggcagt cgattccggt
    27961 gaatctctac tacctgacgg cctttgttgg tgcagatggt cgtacccagt atcgtacaga
    28021 tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca
    28081 attaatcagg taaatgaagt agttcgggga aatcagttgt cgtaataatt agcatgattg
    28141 ggggcgattc tctgcagccc ccgtcactgc tggggttgag tcatcttgac gtctgcttta
    28201 cgggcggtta aggtgcctct tgtgcgccag aagtgcatat aaacgataac attgacctgt
    28261 agacttgatt atcatggaca aattcgacgc taatcgccgc aaattgctgg cgcttggtgg
    28321 cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt gcaacactct ctaccccacg
    28381 cccgcgcatt ttgacactca ataatcttca taccggagag tcaatcaaag cggagttttt
    28441 cgatggcaga ggctatattc aggaagaatt ggcaaaactt aaccattttt tccgcgatta
    28501 ccgcgcgaac aaaataaagt ccatcgaccc aggattattc gaccagttgt atcgcctgca
    28561 agggttgtta ggcacgcgca aaccggtgca actcatttcc ggttatcgtt ctattgatac
    28621 caacaatgaa ctacgcgccc gcagccgtgg agtagcgaag aaaagctatc acactaaagg
    28681 ccaggcgatg gatttccata ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt
    28741 atctatgcgc gcaggtggtg taggatatta tccacgtagt aactttgtgc atattgatac
    28801 cgggccagca cggcactggt agtaattgct taacgaaaca ggggcagtat gaactatcgt
    28861 attattccgg tcaccgcatt ctcccagaac tgttcattaa tctggtgtga acaaacccgt
    28921 ctgaccgcac tggtcgatcc tggcggcgat gcggaaaaaa tcaaacagga agttgatgac
    28981 agcggcctga cactgatgca gatcctgctg acgcatggtc atctggacca cgttggcgca
    29041 gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg gcccggaaaa agaagatgag
    29101 ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg
    29161 ctgacgccag atcgttggct gaacgaaggc gataccatca gcatagggaa tgtgacttta
    29221 caggtgttac attgccctgg gcatacgccg ggtcatgtcg tgttttttga tgatcgggca
    29281 aagctgctga tttctggcga tgttattttc aaaggcggag tagggcgcag tgacttcccg
    29341 cgtggcgatc ataatcaact gatttcttca atcaaagata aattgctgcc actgggggat
    29401 gacgtgacat ttattccggg tcacggacca ttatccacac ttggttatga acgcctgcat
    29461 aatcccttcc tgcaagacga aatgcccgtc aggtaaggca cataaaaaag cccgctttta
    29521 atgcgggcct ggatttctgg caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc
    29581 ttatccggcc tacaaaatcg tgcaaattca aaatattgca ggggacgcgt aggcatgata
    29641 agcgtagcgc atcaggcaat gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc
    29701 ttcattgttt ttaatgctta cagcactgcc acaatcgctt cgcacagcgg agccatgtta
    29761 tctggtgtca tcccggccac atttacgcga ccagaagcaa ccgcatatac gccaaactct
    29821 tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt
    29881 ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt gacgaacaac
    29941 tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca aatcgcacgt
    30001 aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag
    30061 tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt ttcactgtcg
    30121 gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag
    30181 gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct
    30241 tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc
    30301 tcaactgaga gttgtgccag tgtctgccat tgttccagcg tagggtcgat accggttggg
    30361 ttatggcagc agccatggaa cagcactacg tcgccagcct gagcttcgtt caggctgtta
    30421 atcagtgcat cgaagtcaag ggtgtggttt tctgcatcat aataagcgta ttcacgaact
    30481 tccagacctg cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac
    30541 actcgcttaa cgctggtatt ttttgccagg aaatcggcag ctacgcgtag tgcgccagtg
    30601 ccccccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca
    30661 aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt
    30721 ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt caataccggg
    30781 gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca
    30841 tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc
    30901 tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg
    30961 agatttacaa aattccaact attactgatg aaaacgcagg ctgtttttgc aagacgtgag
    31021 attgctctgg aaggcataaa aaaaacagga ccaaagtcct gttttttcgg catttaacaa
    31081 agaggtgtgc tattagaact ggtaaacgat acccacagca acggtgtcgt cagaacctac
    31141 gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt
    31201 tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat
    31261 accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc
    31321 gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctgatgtt
    31381 tgtaaattta ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta
    31441 gatgttgttc gcgtcatact tcagaccagt agcccactgt tcagcttttt taccgttgcc
    31501 aagaagttga gcttcttgca ggttggtacg gtcagctgca ccataagcac caacgatacc
    31561 aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag agcggcgtgc
    31621 agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat caaccagacc
    31681 aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc
    31741 actgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata
    31801 aaccacaccg tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc
    31861 gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg
    31921 gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgag tttccccttt
    31981 aaaaccaaga cgggcatagg tcatgtcgcc attgccaccg taactgtttt caccgttacc
    32041 cttggagaaa taatgcagac cgacagcttt accgtacaga tctactttgt tgccatcttt
    32101 gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc
    32161 cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca
    32221 ctgccgtcaa taagttctgt caataaaaat ttacggaact attgatgaga gtttggtgtc
    32281 tttatgtgtc tgcaggcatc tttccattca aactaacgtt tcgctaccgt gaaagtgcta
    32341 caaagataaa gatttagttt caaaaagaaa aaatatgtaa ccaaaagtaa aaattaagga
    32401 actttgtgaa caccgtcata tttccataga aacgtgatga tatttacagc aatttttatc
    32461 tatttatatg atttacttat atttaaatta actaaacgga aattttgttt ctgatggaaa
    32521 ctttatcgac ctggcacaaa atcctctttc agaca
//
LOCUS       DAZKIV010000034        32553 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZKIV010000034 DAZKIV010000000
VERSION     DAZKIV010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32553
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.034"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            164..2542
                     /gene="ppsA"
                     /locus_tag="ACHOT6_001641"
     CDS             164..2542
                     /gene="ppsA"
                     /locus_tag="ACHOT6_001641"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFP9946878.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYISDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(2599..4245)
                     /gene="fadK"
                     /locus_tag="ACHOT6_001642"
                     /pseudo
     CDS             complement(2599..4245)
                     /gene="fadK"
                     /locus_tag="ACHOT6_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
     gene            complement(4302..4595)
                     /gene="ydiT"
                     /locus_tag="ACHOT6_001643"
     CDS             complement(4302..4595)
                     /gene="ydiT"
                     /locus_tag="ACHOT6_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9946879.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDTGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(4592..5881)
                     /gene="ydiS"
                     /locus_tag="ACHOT6_001644"
     CDS             complement(4592..5881)
                     /gene="ydiS"
                     /locus_tag="ACHOT6_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9946880.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAASAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNNDSVSLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKREQEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(5937..6875)
                     /gene="ydiR"
                     /locus_tag="ACHOT6_001645"
     CDS             complement(5937..6875)
                     /gene="ydiR"
                     /locus_tag="ACHOT6_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFP9946881.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQTV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(6895..7659)
                     /gene="ydiQ"
                     /locus_tag="ACHOT6_001646"
     CDS             complement(6895..7659)
                     /gene="ydiQ"
                     /locus_tag="ACHOT6_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFP9946882.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            7975..8886
                     /gene="ydiP"
                     /locus_tag="ACHOT6_001647"
     CDS             7975..8886
                     /gene="ydiP"
                     /locus_tag="ACHOT6_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9946883.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(8929..10080)
                     /gene="ydiO"
                     /locus_tag="ACHOT6_001648"
     CDS             complement(8929..10080)
                     /gene="ydiO"
                     /locus_tag="ACHOT6_001648"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFP9946884.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(10094..11689)
                     /gene="ydiF"
                     /locus_tag="ACHOT6_001649"
     CDS             complement(10094..11689)
                     /gene="ydiF"
                     /locus_tag="ACHOT6_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFP9946885.1"
                     /translation="MKPVKPPRINGRVPVLSVQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQEMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(11833..12591)
                     /gene="aroD"
                     /locus_tag="ACHOT6_001650"
     CDS             complement(11833..12591)
                     /gene="aroD"
                     /locus_tag="ACHOT6_001650"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFP9946886.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIASVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(12622..13488)
                     /gene="ydiB"
                     /locus_tag="ACHOT6_001651"
     CDS             complement(12622..13488)
                     /gene="ydiB"
                     /locus_tag="ACHOT6_001651"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFP9946887.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVY
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(13500..14765)
                     /gene="ydiN"
                     /locus_tag="ACHOT6_001652"
     CDS             complement(13500..14765)
                     /gene="ydiN"
                     /locus_tag="ACHOT6_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946888.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(14992..16206)
                     /gene="ydiM"
                     /locus_tag="ACHOT6_001653"
     CDS             complement(14992..16206)
                     /gene="ydiM"
                     /locus_tag="ACHOT6_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946889.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(16306..16662)
                     /gene="ydiL"
                     /locus_tag="ACHOT6_001654"
     CDS             complement(16306..16662)
                     /gene="ydiL"
                     /locus_tag="ACHOT6_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFP9946890.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(16798..16905)
                     /gene="rprA"
                     /locus_tag="ACHOT6_001655"
     ncRNA           complement(16798..16905)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHOT6_001655"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(17091..18203)
                     /gene="ydiK"
                     /locus_tag="ACHOT6_001656"
     CDS             complement(17091..18203)
                     /gene="ydiK"
                     /locus_tag="ACHOT6_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFP9946891.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            18592..21648
                     /gene="ydiJ"
                     /locus_tag="ACHOT6_001657"
     CDS             18592..21648
                     /gene="ydiJ"
                     /locus_tag="ACHOT6_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFP9946892.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            21645..22055
                     /gene="menI"
                     /locus_tag="ACHOT6_001658"
     CDS             21645..22055
                     /gene="menI"
                     /locus_tag="ACHOT6_001658"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFP9946893.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGLLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            22155..22343
                     /gene="ydiH"
                     /locus_tag="ACHOT6_001659"
     CDS             22155..22343
                     /gene="ydiH"
                     /locus_tag="ACHOT6_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFP9946894.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            22891..23259
                     /gene="sufA"
                     /locus_tag="ACHOT6_001660"
     CDS             22891..23259
                     /gene="sufA"
                     /locus_tag="ACHOT6_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFP9946895.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            23268..24755
                     /gene="sufB"
                     /locus_tag="ACHOT6_001661"
     CDS             23268..24755
                     /gene="sufB"
                     /locus_tag="ACHOT6_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFP9946896.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            24765..25511
                     /gene="sufC"
                     /locus_tag="ACHOT6_001662"
     CDS             24765..25511
                     /gene="sufC"
                     /locus_tag="ACHOT6_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFP9946897.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRCYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            25486..26757
                     /gene="sufD"
                     /locus_tag="ACHOT6_001663"
     CDS             25486..26757
                     /gene="sufD"
                     /locus_tag="ACHOT6_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFP9946898.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            26754..27974
                     /gene="sufS"
                     /locus_tag="ACHOT6_001664"
     CDS             26754..27974
                     /gene="sufS"
                     /locus_tag="ACHOT6_001664"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFP9946899.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            27987..28403
                     /gene="sufE"
                     /locus_tag="ACHOT6_001665"
     CDS             27987..28403
                     /gene="sufE"
                     /locus_tag="ACHOT6_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFP9946900.1"
                     /translation="MAVLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            28552..29556
                     /gene="ldtE"
                     /locus_tag="ACHOT6_001666"
     CDS             28552..29556
                     /gene="ldtE"
                     /locus_tag="ACHOT6_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFP9946901.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVAVSSGATPTASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(29620..29856)
                     /gene="lpp"
                     /locus_tag="ACHOT6_001667"
     CDS             complement(29620..29856)
                     /gene="lpp"
                     /locus_tag="ACHOT6_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFP9946902.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(30167..31579)
                     /gene="pykF"
                     /locus_tag="ACHOT6_001668"
     CDS             complement(30167..31579)
                     /gene="pykF"
                     /locus_tag="ACHOT6_001668"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFP9946903.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            complement(31587..31781)
                     /locus_tag="ACHOT6_001669"
     CDS             complement(31587..31781)
                     /locus_tag="ACHOT6_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946904.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            32136..32345
                     /gene="fumD"
                     /locus_tag="ACHOT6_001670"
     CDS             32136..32345
                     /gene="fumD"
                     /locus_tag="ACHOT6_001670"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFP9946905.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         8321 a         7955 c         8237 g         8040 t
ORIGIN      
        1 gtcgaagaga gcagatttgc gcaacgctgg gatcagtctt aaaaagtaaa aaaatatatt
       61 tgcttgaacg attcaccgtt tttttcatcc ggttaaatat gcaaagataa atgcgcagaa
      121 atgtgtttct caaaccgttc atttatcaca aaaggattgt tcgatgtcca acaatggctc
      181 gtcaccgctg gtgctttggt ataaccaact cggcatgaat gatgtagaca gggttggggg
      241 caaaaatgcc tccctgggtg aaatgattac taacctttcc ggaatgggtg tttccgttcc
      301 gaatggtttc gccacaaccg ccgacgcgtt taaccagttt ctggaccaaa gcggcgtaaa
      361 ccagcgcatt tatgaactgc tggataaaac ggatattgac gatgttaccc agcttgcgaa
      421 agcgggcgcg caaatccgcc agtggattat cgacactccc ttccagcctg agctggaaaa
      481 cgccatccgc gaagcctatg cacagctttc cgccgatgac gaaaacgcct cttttgcggt
      541 gcgctcctcc gccaccgcag aagatatgcc ggacgcttct tttgccggtc agcaggaaac
      601 cttcctcaac gttcagggtt ttgacgccgt tctcgtggca gtgaagcatg tatttgcttc
      661 tctgtttaac gatcgcgcca tctcttatcg tgtgcaccag ggttacgatc accgtggtgt
      721 ggcgctctcc gccggtgttc aacggatggt gcgctctgac ctcgcatcat ctggcgttat
      781 gttctccatt gataccgaat ccggctttga ccaggtggtg tttatcactt ccgcatgggg
      841 ccttggggaa atggtcgtgc agggtgcggt taacccggat gagttttacg tccataaacc
      901 gacactggcg gcgaatcgcc cggctatcgt gcgccgcacc atggggtcga aaaaaatccg
      961 catggtttac gcgccgaccc aggagcacgg caagcaggtt aaaatcgaag acgtaccgca
     1021 ggaacagcgt gacatcttct cgctgaccaa cgaagaagtg caggaactgg caaaacaggc
     1081 cgtacaaatt gagaaacact acggtcgccc gatggatatt gagtgggcga aagatggcca
     1141 caccggcaaa ctgttcattg tgcaggcgcg tccggaaacc gtgcgctcac gcggtcaggt
     1201 catggagcgt tatacgctgc attcacaggg taagattatc gccgaaggcc gtgctatcgg
     1261 tcatcgcatc ggtgcgggtc cggtgaaagt catccatgac atcagcgaaa tgaaccgcat
     1321 cgaacctggt gacgtgctgg tcactgacat gaccgacccg gactgggaac cgatcatgaa
     1381 gaaagcatct gccatcgtca ccaaccgtgg cggtcgtacc tgtcacgcgg cgatcatcgc
     1441 tcgtgaactg ggcattccgg cggtagtggg ctgtggtgat gcaacagaac gaatgaaaga
     1501 cggtgagaac gtcactgttt cttgtgccga aggtgatacc ggttacgtct atgcggagtt
     1561 gctggaattt agcgtgaaaa gctccagcgt agaaacgatg ccggatctgc cgttgaaagt
     1621 gatgatgaac gtcggtaacc cggaccgtgc tttcgacttc gcctgcctgc cgaacgaagg
     1681 cgtgggcctt gcgcgtctgg aatttatcat caaccgtatg attggcgtcc acccacgcgc
     1741 actgcttgag tttgacgatc aggaaccgca gttgcaaaac gaaatccgcg agatgatgaa
     1801 aggttttgat tctccgcgtg aattttacgt tggtcgtctg actgaaggga tcgcgacgct
     1861 gggtgccgcg ttttatccga agcgcgtcat tgtccgtctc tctgatttta aatcgaacga
     1921 atatgccaac ctggtcggtg gtgagcgtta cgagccagat gaagagaacc cgatgctcgg
     1981 cttccgtggc gcgggacgct atatttccga cagcttccgc gactgtttcg cgctggagtg
     2041 cgaagcagtg aaacgtgtgc gcaacgacat ggggctgacc aacgttgaga tcatgatccc
     2101 gttcgtgcgt accgtagatc aggcgaaagc ggtggttgag gaactggcgc gtcaggggct
     2161 gaaacgtggt gagaacgggc tgaaaatcat catgatgtgt gaaatcccgt ccaacgcctt
     2221 gctggccgag cagttcctcg aatatttcga cggcttctca attggctcaa acgacatgac
     2281 gcagctagcg ctcggtctgg atcgtgactc cggcgtggtg tctgaattgt tcgatgagcg
     2341 caacgatgcg gtgaaagcat tgctgtcgat ggctatccgt gccgcgaaga aacagggcaa
     2401 atatgtcggg atttgcggtc agggtccgtc cgaccacgaa gactttgccg catggttgat
     2461 ggaagagggg atcgatagcc tgtctctgaa tccggacacc gtggtgcaaa cctggttaag
     2521 cctggctgaa ctgaagaaat aaaataaata cccggcggcg ttttgtcgcc gggtttatgt
     2581 gatccccgaa gatgaaactt attcaatctc ttcacagaca tcctgagtta atcgccgcat
     2641 aatatctttc cttaacaaaa acttttgtat tttacctgag gcagtgcgcg gtagtttttc
     2701 gattaccacg atatgttcag gatatttata ttttgcgacc cgtttacggc taaaaaaagc
     2761 caccacctct tccagcgata atgaatgatg cggcgctttc agcacgacat aagcgcatga
     2821 tcgttcacct aaacgttcat cgggcattgc aaccacacag gcatcgtgaa ttttaggatg
     2881 ctgcaataaa atatcttcca cttcaaggct gctaatattt tcgccgccgc ggacaataat
     2941 atcttttttg cgtccggtaa tttttatata gccagcctca tccatacggc agagatcgcc
     3001 gctgtaatac cagccttctt catccagcgc acgggcggtt aattcaggtt catcaaaata
     3061 tcccataaac acattggggc cacgcgaggc ttcttcacct tcgcaacctg gcggtaaggt
     3121 cttgcgtgcg tcatcgacca ctttaatctc tacacctgcg gcagcgtaac catcggtgtg
     3181 cataaagcgc gacaaaggat catcgagatt caccaccgca tgcggcgaac tttctgtgga
     3241 accataaaca cttaataatt taatgctgcg ctgctggcat tcacgcgcca cttttttggg
     3301 gattgtggtg ccaccgcaaa gaaagaagcg cagcgctgaa aggtctgcgg gctgtttctc
     3361 tagcaaattc aaaagatcat agacaaacgg cgttgcgccg agcatacagg tgcaacgctg
     3421 ctgctcaagc agcgcgagac acgcatcagg agtgaaaata tctaacaaca cgctgcgagc
     3481 accaatcaaa aatggtgctg ttacgccatg cagaaagccc gttgcgtggc cgagtggcgc
     3541 aggcatcata aagacatcct gccaggtcag attcagccgc gcgcaataag cccgctcact
     3601 ggcgagaata ttgttatgcg ttagcatcac gccctttggc agaccctcgg ttccggaggt
     3661 aaacagcacc gcagctaatt catcgccgtg ggtcgttatc gccgtggtca gtggggtatt
     3721 gtcggcgata atctgactta attagaggga agaggtggcg ggagccagtt tgtccacgcc
     3781 gacaatttgt tgtagttgtg gaagctgatt ttgcagcggc aggattaaat ctaccgggct
     3841 cgtttgttta aacaacgtcg gtgcaaagaa catttttgcc tgacacttat tgagcaccca
     3901 caccagttct gcttcccgcc aggaaggcaa cagcggcacg gaaaccgcac cgattttcag
     3961 gcaggcaaga tagataacgg taaattcaca ccagccaggc agttgaaatg cgatgcgatc
     4021 gcctgattca atacccttcg ctaacatcca gtttgccaga cagctcgcgg cgtgatcgag
     4081 cgcgctatag gtgtacgatg caccatgatt atcgaccacg gcaattttgt ctggcatcgc
     4141 acgagcggtc tgctgccagt aatcggccag cgaagcatcg ccccatagcc cttgctgacg
     4201 atacgccgca cgacgttgtt cgttaaacgt taatgtcact ttcattttgg actctcgaaa
     4261 cagaacatca ggcccgagat gcgggcctgt gggatgcatg attagccgta gcgaaaatca
     4321 ataccgaagg tgcctgcggg atattgccac tgttcgagaa tagtgttacc gcacagcacc
     4381 cgacaggtgc cgcactccag acaaccggcg gaatcaaaat gaatgtttcc tgtgtcatcc
     4441 tgcttataaa gtccggcagg gcaggctttc attaatttac ggaattcatt gatatcggga
     4501 tttgccgcca gaatgatatg cgggtggccc tcatcaacat ggaatttatt gacgcctaat
     4561 ttgatgtcga cgttaaccgt agcgttctgg ctcatagcgc ggttgctccc ttaatgccat
     4621 ctttcagcaa gttgatcagc ccaattttct tcgcgtgtcc catgatcatt ttgcgtaccg
     4681 gctggtttgg tttgccgtca atggtgaaca tctcgttcat gatgtcggcg accattcgtg
     4741 ggtattggct aaacaggcgc gggttttcca tcagcgccgg gatcttgcgg aaatgctgca
     4801 tatcgcgcat gacgcagctt tgttcctgct cacgtttgta ttgcgccaga ctgctggcgg
     4861 agaaatcctc gcgttctttg gcggcgatca ccgttgtggc ggcagcctga gccgatgcaa
     4921 tggctaaatc catgccacga actgtaaaac ccaaattcag gcagaagcct gcggcgtcac
     4981 caacgatcat cacgccatcg ttaaccagct gcggcaccat tgccagaccg ccttctggca
     5041 ccatatgcgc ggaatattca agcagtttgc cgccgctaat tagtgggcga atggcggggt
     5101 gttgtttaaa atcttccagc atttgcggca cgcttttttg cgcatgggcg atatcaccca
     5161 atccacaaac cagccccaac gatacggaat cgttgttggt atagaggaat ccgccaccca
     5221 tcaggccgtc agaaggggaa ccggcaaaca gccaggcggc accttcctcg cccgtaatat
     5281 taaagcgatc gttgatctgt tctggcgtga ggccaataac ctctttaaca ccaacagcgt
     5341 aatgatgcgg atcggaagcg ggaaccattc ccagcgagcg gccaagcatc gagttaacgc
     5401 catcagccag aatcaccaca ttcgcttcga gaatatcatc cccagcctgc acgccagtta
     5461 ccttgtttcc ttcacgaacc agcgcatcga cgcgaacgcc tgggataaac tgcgcgcccg
     5521 cctgctcggc ttgttccatc aaccacgggt ccagacgatt acgcaatacg gtataagatg
     5581 cgtgttgcgg aacatctggc tgctcgcggt gaaaatcgag ggtaacggcg ctttcttcgg
     5641 ttaagaagga gattttctcg cgtgtgacct tgcgttctac cggcgctgat gctgcaaagc
     5701 ctggaatgat tgcttcaagt gtgtgggcat aaagacgccc gccggtcatg tttttacatc
     5761 cggcactgtc gccgcgttct atcaccagga catccagtcc ggctcgtgcc atgaccagtg
     5821 cggcaacgct accagcaacg cccgcaccga ccacaatggc atcaaatttg tcatccgaca
     5881 tgcgttatgc tcccttcccg ctgcgtttac agcgggagat gtggttgaaa ggggaattag
     5941 cggctcaact ggctaatcag ggcagggacg actttgtaga tatcgcccac cagaccgtag
     6001 tcggcatagt tgaagattgg cgcattttta tctttattga tggcgacaat cacttttgcg
     6061 ccgttgccgc caaccatatg ttggatctgc ccggagatcc ccagcgtcag gtagagatca
     6121 gatttcagca acacgccgga gacaccgata taacgttcac gctccatcca gttctcgcct
     6181 tcggcaattg gacgtgaaca gccgacttca gcattcagca ccgccgccag ttcgtggacc
     6241 attttcaggt catcctgcgc cgcaagacca cgaccgacgc caaccacacg ttttgctttg
     6301 ctcaggtcca cgctgcttgc ggctttcgca cggcgttcgc gacagagaat ttcatgacgc
     6361 ggagcaacat aaggtaccgt ttctgtcggg cactgatgag aggtatcact ggtgcacggt
     6421 tcctgaacac cgggtgcaag ggtaatgatc gccagcgggc tgttgatctt ttcctgagcg
     6481 aacgctaacc cgccatacat ccggtgttcg gcgcaaatgt gaccatcgac aatatccacc
     6541 gccgtggcat cgttcaccag cgccgcattc agttgcacac ttaaccgtgc tgccagagct
     6601 ttaccacgtt tcgtcgcggc caacagcaac atagcggggt gtttatcttt cagcagggca
     6661 gcaatgcttt cggcgtaatt ttcagtgcgt tgcagcgcgt cgttttgctc aagaacataa
     6721 atacattttg gaccataagg cataactgtc tgcgcctggt cggtattttg tacaatggca
     6781 tacacctgtt ggccccattg ctgagcgccg ccaaacagtt cagcataacg ttcaggatta
     6841 tcgctaaaga cccagacgct gtttaattga ctcatcgttt cttctccgta ggcttcagtt
     6901 cagggctttc ttcagatgtt ccgccagctc ggcaatggcc tccggcgaat cgttatcgat
     6961 aatgatgtgc ttacgttctg tttgcggcgg tacgcgaatg ccgaccagtt cagcaagtgg
     7021 cgcgctctgg ctccagtcaa tatcacttgc ctgccactga tttaccggtt ttttacccgc
     7081 gccgagaatg gctttcatcg aaggaatgcg tggcacgtta atatcggagg tgacgcagag
     7141 cacggctgga acagagagtt caataacttc aacatcatct tcaagcgtgc gttcaatcac
     7201 cagtgtattg ccctgacgct gaatagcact cactgcatta atcaccggaa gttgcagaat
     7261 ttctccgacc agcaagccaa cctgctgggc ataaaggtcg ccggaacctt caccaaagat
     7321 cagtaaatcg aagccgatct tttcaattgc tgccgccagc gcttttgcgg tatcgagagg
     7381 cagtgcatgt tcaagttgcg catcctgcac caaatacagg ctgtgcggcc cgcgggatag
     7441 cacgtctttg cgcactttcg agttctgcaa caatgagccg ccaacggtca gcgcggctat
     7501 ctcatcgtca tctgttgcga gctggcttgc agcttcaatg gcattgagat cgaactggct
     7561 gattttggcg tcggcattgt cgaaattcag ggtgtattct ggagtgacaa caatgtcctg
     7621 ttcttcaggc accagcttaa agcaggttat tattttcatg atttctcctg tgagtcgcat
     7681 acggaaccat ctccgatgga aggtaaaaat ggcctgaaaa tagtgtgcga tagcggtgcg
     7741 gggttttcga cggagcaagc tgttttcctt gccaacaatc ttgcaaaaaa aatgcgatcg
     7801 ctaaaacaaa agtgcaattt gcggcaatat agttattcca tgaccgtaca atcttgcatt
     7861 cagtgtaaat aatcttgttt agctctcgct aaacgcggtt gtataaccgc aatacacgcc
     7921 gtattttatc cggcatatat tgtttttgct tactcatgga atagggaggt tgtcatatat
     7981 caacgctgtt ttgataacgc cagtgaaacg ctgtttgtcg ccggtaaaac gccacggctt
     8041 tcacgttttg catttagcga tgatccaaaa tgggagtccg gacatcacgt tcatgacaat
     8101 gaaaccgagt tgatttacgt caagaaaggg gttgcaaggt ttaccatcga ttcttcgtta
     8161 tatgtcgcac atgcggatga cattgtggtg atagaacgcg gcaggctgca tgcggtggcc
     8221 tctgacgtta acgatccggc aacgacgtgt acctgtgcgc tgtacggctt tcagtttcag
     8281 ggggctgagg aaaatcagct actgcaaccg cattcttgtc cggtaattgc cgcggggcag
     8341 ggaaaagaag tcataaaaac cttatttaat gagctaagtg tgattttgcc gcaaagtaaa
     8401 aatagccaaa catcttcgtt atgggatgca tttgcctata cattagcaat tctttactac
     8461 gaaaacttta aaaatgctta tcgttcggag caggggtata ttaaaaaaga tgttctgata
     8521 aaagatattc ttttctatct gaataataat tatcgcgaaa aaatcacttt agaacagtta
     8581 tcgaaaaaat ttcgtgccag cgtcagttat atttgccatg aatttaccaa agagtatcgt
     8641 atttccccta ttaactatgt tattcaacgg cgtatgacgg aagcaaaatg gtcactcacg
     8701 aatactgaat tatcacaggc agagatctcc tggcgtgtgg gttatgaaaa tgtcgatcac
     8761 tttgccaaac tgtttttgcg ccatgtcggt tgttcgccca gcgattaccg tagacaattt
     8821 aaaaactgtt ttgcggaaca agaaatccta tctgaatttc ctcaaccggt aagtcttgtt
     8881 ggataaatct ggcggcgcag tttgttaaga agctgcgccg cctgcagatt atttgttctg
     8941 atagtctttc aggatctgcc gacctgctac gtaaatcata atttcgtctg taccgccgcc
     9001 gatacgttca caacggacat cacgccagaa gcgggagacg cgcgcttcat cggtatagcc
     9061 cagaccgccc atgatttgaa tcgcatcatc aatgacttcc attgcggtac gtgcgcaata
     9121 cagttttgcc agcgccgcgc tggtgcgcag tgactgatgc tgatcggctt gccatgccac
     9181 tttcagcacc atgttgcgca tgttgtcgat cttaatcgcc atcagcgcca gtttttcctg
     9241 gatcatctgg ttatgaccaa tgggcttacc aaaagcgata cgttggttgg catagcgggc
     9301 ggcatcttca aaggcgcatt cggcaaagcc ggtgctgcgc gcggcgttga tcaggcgctc
     9361 catctcaaag ttgtacatca cattgaggaa acccattcct tcttcgccca ccatgtcgct
     9421 ctcttcaact tccacattgt cgagatagac ttcgcaggtg ctgagcatat gccagccgat
     9481 tttatgcagc gggttaatct taatgccggg cttactggag tcgacccacc acagggtgaa
     9541 ggcttttttg ggatctttcg gttgcggatc gcgcgccagc accagcatat acgggtactc
     9601 tttcgcaccg gtaataaagg ttttttgtcc gttgatgtag accttgccgt ttttacgcgt
     9661 gtaagtggta gtggcactgt tgttatctga gccagcacct ggttccgtca acgccagggc
     9721 ataggcggga tcacccgttt ccagggtgct ctctgccgtt ttacgtagct gctctgcaga
     9781 accgaaacgg cgcatactgt gaatacattg accgttggta attaaaaatg ccggagcacc
     9841 gcattttgac acttccatca gcgccagcat ttgggtgacg taatccgcag ggataccacc
     9901 aaattcttcc ggcacgccaa gcatggaaat accgttatcc gccagcgccc gcataaactc
     9961 acgcggatat gtcccgtttt gatcgcaggt gcggaaatac tcttccggaa agttagtcgt
    10021 aatcagttcg cgaatactgg ccagcagcag ttcttgttct tcagttaaag aaaaatccat
    10081 cattatactc ctgttaatga gccgcttcag gcaggacaaa acccatcgcc gcatcgataa
    10141 ataatctttc gtccatcagt ttgagttctg gcgaaatcac tggggtgaag tccattttgt
    10201 cgagaatatc tttttgtaaa tcgacgccag gggcgatttc gattaaatgc aggccgtctt
    10261 ctttcagcgt gaatacagca cgttcggtga tgtaacgaac atccagtccc cgttcgagcg
    10321 cgatttttcc gctaaaagta atttccggta gttcccgaat aaatttattt acccgtcctt
    10381 cctggacgat atgcaatttg ccatcggcaa tttctgtttt taaactgccc gcagttaacg
    10441 tgccgcaaaa aataattttc ttcgaggtgg cactgatatc aataaatcca ccggtaccca
    10501 tgattttgcc attgaattta tgcacgccga cgttaccgtg ctggtcgact tcagcaaaac
    10561 tcaaataaca aacatccaga ccaccaccgt gataaaaatc aaactgggac gtcatatcca
    10621 gaatggcacg ggtattcacg ttcgcgccaa aggcgatccc ctgtgaagta attccgccaa
    10681 tcggacctgt ttctaccgtc agaataaagt catcagcaca accttcttcc cgggcgacca
    10741 ggccaatacc gtcagcaata ccgacgccga cattccccac cgcgccttta cgcatttcga
    10801 ataatgcacg cctcgcaact aatttacgtt ggtttagggg caggctaagt ttggtgctgt
    10861 catcaagggt gaagtcacca gaaataaagc ggttaaccgg cgcaccgcca tacagttggc
    10921 tttgatccgg atcgaccacc acaatatcca ccagataacc cggaatacgg acagatttag
    10981 gatgcagcgt ggctttctta accatttcct gcacctgcat catcacaata ccgccgttat
    11041 tgtgcaccgc ctgggcaata accaatgcgt cgagatacat cacctcatct tcaaaagtgg
    11101 cgtagccttc actgtcgcag gtggtagcgc gaatgaaggc gatatctggc gcaatcgctt
    11161 tgtaatagag atattctttg ttatcaaact cgaccagttt aatcaggtct tctttcgtga
    11221 cttcattcag tttgccgcct tgctggcgtg gatcgacaaa tgtcccgatg ccaatatcac
    11281 taataatacc aggctggtgg gctgcggcgg cgcgtaaggt ttgtgtaagt acaccttgtg
    11341 gatagttata agcaataatt ttattttgtt ctgcgagttc agaaatacgc ggcgattgtc
    11401 cccagtgacc acataatgcc catttcacca gaccttcttg cgccagagga ctaataccac
    11461 ggtcggcgcg atcgccaagc cctgtcggac taataatcga taaattacgt ggcgtttgag
    11521 tctgtttata tttatcagca agagctgtaa ttaacgtggt ggcttccaga ataccgccgc
    11581 cagcgcctaa cacacaaagt gttgcttcgt cgggaatata attcaccgct tcctgtaccg
    11641 acaggaccgg cactcgtcca ttaatacgag gtggttttac aggtttcatt gttaattcct
    11701 gattcagatg tgattttccc tacgccagat ggagaattag cgcacagaga ctcacgaaat
    11761 atttttatta aatacggtta aatatatttt ttaagttcgg cggggagggt gttcccgccg
    11821 aaatattatt gcttatgcct ggtgtaaaat agttaatacc gtgcgtaaat catttaccga
    11881 gatttgccct ggtgcagagg ctttttttac cgcaccaaaa gttgccgccg agccaaatac
    11941 ttcaccagcc agacgagaaa ttacgccagt ttttgccatc gacatcgtga tgattggacg
    12001 atcggcatac tgctcctgca tttccagggt cgcggtaagc aacgtcagca catcgctggt
    12061 actttgcggc atcagcgcaa tcttaggaat atcggcgtcg aaggactgca ttttgcgcag
    12121 acgggcaatg atttcttcgg cttccggcgt tttatggaag tcatggttgg acatgacaac
    12181 tttcacatca tgcgcgtggg cgtaggcgac ggtttcttta acctgatcat cgccggtaaa
    12241 taactccaga tcgatcatat caaccaggcc gctgtcgatg gctgcacgat tgagagcaat
    12301 ataagcctcg gtggaaatcg cctgctcgcc gccttctttg gcactgcgga aggtaaacag
    12361 cagcggtttt tctggcatgg tttcacggag aatttttgcc gccgccatga cagactccac
    12421 attggagagg tcggcaaagt ggtccacacg ccattccaga atatcaaagt ccgcttcacg
    12481 ataggcgaga gcttcggatt tcacgctggc gatatctttc gccatcagcg agacgatgat
    12541 tttaggtgcg cccgtaccaa tgacgagatc ttttacagtt acggttttca ttttttaccc
    12601 tttctgcacg cggtcagcct gtcaggcacc gaaccccatg acctgtttaa catattccag
    12661 agggaaatct ttgccagtcc ataacgtgaa ctgttcagcc ccttgccaca acaacatgcc
    12721 gtatccatca atcgttttgc aaccagcttg ttgtgcctgc tgcaataact tcgtcatatg
    12781 cgggttatac acgcattcag tgaccagcag tcccggatgt aacagactga tatcataaac
    12841 caatgattca ttctcaaggg gtttcatacc cacttttgtg ccattggtta aaatgtcggc
    12901 ggaagccagg gcttcagcaa aggcttgctg atcggcgaga tcggtaaccg tgacgacaca
    12961 atcggtgttt tcgttaaccc gctgcgcgaa ggcgagggct ttatcgaaga actcatcccg
    13021 acggttaaag agtttaattt cttttaaacc ttcaattgcc ccctgcgcgc caatcgccgt
    13081 tgaggcacct ccggccccta acagcaccat tgttttgcct ttgatatcaa aaccgctctc
    13141 tttaatggcg cgaatatggc ccgtgccgtc ggtgttatag ccgcgcaggt agccatcatc
    13201 attaacgatg gtgttgatgg cacccaccag tttggctgct ggcgttaatt catcaacata
    13261 ttcacacgcc agttgtttgt tcggcattga tataccagtt ccgcgcattt tgagggcttt
    13321 taatccttca attgctccgg gaaaactatc gttatccact tcaaaggcca tataggtaaa
    13381 tggcaatccc gctttttcta aggctttatt ctgcatttca ggcgataaac tgtggcggat
    13441 aggataggcc atcagcccaa tcaattcgta ttttgcggta acatccataa ttaactcctt
    13501 taacctctat gcttaattgt gtttaaccgg gtgctaaatt tgcgctcgcc aaaccgcaca
    13561 tcattctcag gaataataaa aacgcggtaa tagcggataa aaacaataat tgcggtaatc
    13621 agggccagca gcgcgaaagt aaaatcgaga acaatgatat attgcaggcc gatgttcgac
    13681 agataaccgg taatcagcgg aataacaaag ttagccagtc cacccatcat catataaata
    13741 ctggtgactt tggctttgct ttttgggaaa aactctgaca tgaccgaaac accgagctgt
    13801 aaaatgccgc cagctgctga gaaaccgata acaaaggcgc cggcattaca caccagtgga
    13861 gaagggtaca ggtagataat ggctgctgtt attgttgcca gtgcagagtt aaatacatta
    13921 gcccagatgg gccggaccat ttttttcagt agtgcggcaa aaataaagac acagaccaac
    13981 gagcccatac tgtaataaga gatggttttt aatgcctcag cttctgacat accagcaaaa
    14041 gccatcgcat atttgggcat ccacaccaca atcacataaa aggtcgagaa tgcggctaca
    14101 ccgaacagta ccgatgaaac accttccagc cagactaacg gtttgctgtt catttgcggt
    14161 aattcattag ctacgctggc gtccaccaac tggctgggga atttgctttt caacagcatc
    14221 agcgtgatca gtacaaacag aatacccgga ataatcagcc catagccgta ccagatatta
    14281 ttgagcaaca tatagctcac cagcattggg tagaacattt gcccaaatga caccatcgct
    14341 ttaaccagta tgaccgccga accagaggct ttcggaaagc attccatgag cgcggggtag
    14401 ccacccgtat ccagcgctga gttagcgata cctacgcaca ctgccagacc gtaggcgaga
    14461 gttaaattcg ggcaagcggg aataccaaag aagaatagca gatacattat tactgccatt
    14521 aatatcaccg cccgacgacc aaacttatcg gagatcacac cgaagaataa aatactgatc
    14581 aatcgcccca aaccaatacc ggaaattaag taggcaatgc ccgcgttgtc agtggaaaac
    14641 ttttccgcca gagatgacat gttttgggca agcgtaataa cactaatgcc gtgcaggaag
    14701 tagctgaagt aaatacaaag aacagccagg ataaatggcg tgctgaaagc cttattttga
    14761 gacattttca cagctccttg ctgaaagata atttcgttac cgtacgtatt gttcttcgac
    14821 ggtttacata attactatac tgaagatgat tttccctggg tacatggcat aaagtttatg
    14881 tttccaggaa tattgcatgt catagtttgt ttttgatcgc gattttcctt agttagcgca
    14941 gaaagagtta tttatccgta agatcttgca tgacctgaaa agaatattgc attacccacc
    15001 cggagcgaca ttttccttta gcgagtgatg ccgcgttttt ttgcggctgc gtaagccgat
    15061 aaacagtgcc agtaaaaaac cgatggcagc gatggcggta tcgaaccaca taatatcggc
    15121 aatacttctt tgggacagat gagccgtaat caacggaata gtaaaggtcg caatactgcc
    15181 cgcactgtaa tagatccctg tagctttacc tttagcgtaa gggaaacgtt cagccattaa
    15241 cgtcaggcca atttgcacaa cacctccagc agaggtaaaa ccaatgacaa aagcaaatat
    15301 tatcaccaca taaaatgtgg gatgcaggca gacggtaaac agagcaataa atgagataaa
    15361 ggtgtacagc atcagtaatg ttgttgggcg aacggtatta cgaatgagtg gagcggtaat
    15421 aaatacacaa agcagcgaac ccacggtgta gatactgagt agtttaatcg acatagtgta
    15481 tgacatgcct gcaacaaatt gtccgtactg tgccagccac tggctaacca gataaaacgt
    15541 tgccattgag atatagccat ataaggtata actggctaaa tcgataattg aacagcgatg
    15601 ttccgtagag ctggtggttt tctttatgac aggtaagcga cgacccggat ggggtgggaa
    15661 cgtacaacgg tataaaaaca gagcgttaat aaacataatg cctgcagcaa tcataaagga
    15721 ccaaccgaac cacagttcag cccacactaa caggctaatg attagcggta ataaaaattg
    15781 tccgctggaa acaaatgctt taattaaaat attggctgtc ccaggtgagc gtggaaaagc
    15841 ttccatcaaa ctgggataag tgcctgcatc gagaaaactg tttgccattc ccgccagaaa
    15901 gccaaaaaca taggcgataa tgatgttatt ggtgtgcagg atgccaaaaa agaaggccat
    15961 atagcagcac atcccgagca tgataaaagg gcggcgacca aagcgatcgg ataataatcc
    16021 tgcaaaaagc aagacactta atcgaccaat gcccagcgat gagataacta tcgagacacc
    16081 cgcggcatta gtctgccaaa gtgtctccag cgaggccata ttcaggctca tcaaaatgac
    16141 gcccatacca tgcaccaggt aattaaaata caacccaagt gcggtagggt aatagggatt
    16201 tttcataggt tctacttagc aaaggtgctt taaaaagtgt agcgatgttg tgtcagtatc
    16261 cattcattat tgttgtaaca acgacaattc ctttctgtgg aaaaattaac aaagccgctc
    16321 cagatcgtgt gcatataatt cagcggctac cgattgatag atcttccagt caataaaatt
    16381 gccatcaggg tagactcgtt gaaatgcggt taagtcgggg aaaaaacgca tggtgttgtt
    16441 gccgatgcgg ttatttattt tctctatgat ggcatgaaga tgtttttttc tttgctgacg
    16501 catagccaac agttgctcga ctacacgatc aggaatggca cacttgccat tttcccactg
    16561 acgccagctt tcactgtcac cggtctgggc aatccaggtc gcgcattcat caatagtcat
    16621 ggcaaaaata tggcggagtg cctggagttc ataagcgttc atcgtgtaat ggctgcatga
    16681 aataaacaat aaaatataaa taacattatc agcctgctga cggcttgaag agagtcacag
    16741 tatcttgtgc aacattattg gaaagagtga ggggcgaggt agcgaagtgg aaaaatgtta
    16801 aaaaaaagcc catcgtggga gatgggcaaa gactacacac agcaattcgt tgtttcactc
    16861 aggggatttc catgcttata aatcaatatg ttgatttata accgtgagct aatagtaggc
    16921 atacggactt tttgcgtcga tcagattcgt ctcattagtt taaaaactgt aaagaatttg
    16981 cgacaggtac taccagctga atggcggtaa gcaggaaaat atggggaaga gcaagcaaat
    17041 atcaattagc gggggttaac aatcaggcgg cagagccgcc tgatgagaaa ttacttattc
    17101 ggtttctcga tttcgccaag ttcttcgaga atttcttccg gttggtccgt cggcggcggg
    17161 acttcttcca cccacgcggc aaacagacgc caggaaacgg ctaacagaac cggaccaata
    17221 aacagaccga tcatcccgaa agcaatcaaa ccaccaataa caccagagag gatcaggatc
    17281 agcggtaaat cggcacccat gcgaattaac attgggcgga tgacgttatc cagcgtgcca
    17341 accacaccgc tccacactaa caataccgtt ccccaggtgg tatcgccagt ccagtagagc
    17401 cagataatcg ccggaatcag taccggcaac gggccaagct ggacaaggca ggagaggatc
    17461 attaacaccg ttagcaacgt tgcataaggt acgccggata cggcgaggcc gataccgcca
    17521 agcactgcct gtactaacgc cgtcaccacc acacccagcg ccaccgcgcg gatagcctgt
    17581 gccgccagca gtacggcggc atctccgcga acgcctgcca gacgggttgc aaaatggcga
    17641 atgccttgtg ccacctgttc accgcgccag tacagcaggg cactgaagag aagcatcagc
    17701 gcacaatgca ccataaagcg cccgatatgc gccgcctgcc caacgaacca ggtggtggtg
    17761 gtgccaatat aagggcggac tttcgccatg atcgccgtcc cccccatatc cagcaagttg
    17821 tgccagcctg catacagctt cgcgccaatc accggaatgg tattaagcca cgccagatcg
    17881 ggtaacgtca tgtcgccgct ggaaatggct ttaattagcg ggccgctgcc gtcgacgata
    17941 ctgttaacca gcaaagcaat agggatgata aacaccatca ctaataacag cgtcatcacc
    18001 agaacggcga gagagcggcg gccaaacatg atcttttgca aacgtaacaa taccggccag
    18061 gtggcgataa ccaccgtacc ggcccatgca aagccgagaa taaagggttg aacaatccac
    18121 agacatgcca caatcatgat ggctaaaaac agcaccgaaa gcagaatttg tgcgacatcc
    18181 ctgggctgac gaacatttac cataactact tttcaccttt tttgtgcgtc aaatcgtcga
    18241 cgcgacgtga acacgcgaaa ctcacctgca taatgatgag caatttcagc ggtttttaac
    18301 aggccgattc tgcccttaat tctgatgggc gtgttagaaa aaaatgtggt acaacaactc
    18361 aggcaacacg caaacgattt actcgtcgta tttcaagttg catgtgctgc ggctacgttc
    18421 gttcacccca gtcacttact attgtaagct cctggagttc actcgcttgc cgccttcctg
    18481 caactcgaaa tccatagagt aaataaacat cacctttcag tgcgctattg cagccgcctg
    18541 aaagaatagt gtatcgacct acatcacaga ccgcaggaaa gggtcaatat aatgattcca
    18601 cagatttccc aggcacccgg cgtcgttcaa ctggtgctta attttttgca agagctggag
    18661 caacaaggtt ttaccggcga tacggcgaca agttatgccg atcgtctgac aatgtcgacc
    18721 gacaacagta tttaccaact tctccccgat gcggtggtat ttccgcgttc aaccgcagat
    18781 gtggcgctga tcgcccgtct tgccgcgcag gaacgctatt catcgctgat ctttaccccc
    18841 cgcggcggcg gcaccggcac taacggtcag gcgctcaacc aggggattat tgttgatatg
    18901 tcccgccata tgaaccgcat catcgaaatt aaccctgaag agggctgggt gcgcgttgag
    18961 gccggggtga taaaagatca actcaatcag tacctgaaac cgttcggcta cttttttgcg
    19021 ccggaacttt cgaccagcaa ccgggcaacg ctcggtggga tgatcaatac cgatgcttct
    19081 ggtcagggat cgctggtcta tggcaaaacg tcggatcacg tacttggcgt acgtgcggtg
    19141 ttgttggggg gcgatattct cgatacgcaa cctttacccg tcgaactggc ggaaacgctg
    19201 ggtaaatcca ataccacaat cgggcgaatt tataacacgg tttatcaacg ttgccgtcag
    19261 caacgccagt taatcatcga caacttcccc aaacttaacc gctttcttac cggttacgat
    19321 ctgcgtcatg tctttaacga tgagatgacc gagttcgacc tgacgcgcat tctgacgggt
    19381 tcagaaggga cgctggcctt tattaccgaa gcgcggctgg atattacgcc cttgcctaaa
    19441 gtgcgccgtc tggtgaacgt caaatatgac tcttttgact ccgcgctgcg taacgcgccg
    19501 tttatggttg aggcgcgggc gctttcggta gagacggtgg actcaaaagt gctgaatctg
    19561 gcgcgggaag atattgtctg gcattccgtc agcgagttga ttaccgatgt gcctgacaaa
    19621 gagatgctcg ggctgaacat tgtggagttt gctggtgatg atgaggcgct gattgacgag
    19681 cgagttaatg ctctctgtgt gcggcttgat gagctgattg ccagccaaca ggcaggtgtg
    19741 attggctggc aggtgtgccg cgagctggcg ggtgttgaac gtatctatgc gatgcgtaaa
    19801 aaagccgttg gtctgcttgg caatgccaaa ggtgccgcta agccaattcc gtttgctgag
    19861 gatacctgcg taccgccgga acatctggcc gattatattg ctgaatttcg cgcgctgctc
    19921 gacagccacg gcttaagcta cggtatgttc ggtcacgtcg acgcaggtgt cttgcacgtc
    19981 cgtccagcgc tggatatgtg cgatcctcaa caagagattt tgatgaagca aatctctgat
    20041 gacgtagtgg cgctgactgc gaaatacggt ggtttgttgt ggggcgagca cggcaaaggt
    20101 tttcgcgctg aatacagccc ggcgtttttc ggtgaggaac tttttgcaga actgcgcaaa
    20161 gtgaaagcgg catttgaccc gcataaccga ctcaacccag ggaagatttg cccgccagaa
    20221 ggtctcgatg cgccgatgat gaaagtggac gcggtgaagc gcggtacatt cgatcggcag
    20281 atccccattg cggtacgcca gcagtggcgc ggtgcgatgg agtgtaacgg caacggttta
    20341 tgcttcaact ttgatgcccg tagtccgatg tgtccgtcga tgaagatcac ccagaaccgg
    20401 attcattcac cgaaagggcg cgcaacgctg gtgcgtgaat ggctgcgttt gttggcggat
    20461 cgcggcgttg atccactcaa actggaacaa gaactgcctg aatccggcgt cagtttgcgg
    20521 acgttaattg cccgcacgcg caatagctgg catgcgaata aaggcgaata tgacttctca
    20581 cacgaagtca aagaggcgat gtcgggctgt ctggcctgta aagcgtgttc gacccagtgc
    20641 cccatcaaaa ttgatgtgcc ggagtttcgc tctcgttttc tgcagctcta tcacacccgt
    20701 tatttacgcc cgctgcgcga ccacctcgtc gctacggtcg agagctacgc gccgctgatg
    20761 gcacgcgcgc cgaaaacctt taacttcttc attaaccagc cgctggtgcg caaactctcg
    20821 gaaaaacata tcggcatggt tgatttgccg ctgctgtcgg tcccctcgct acaacaacaa
    20881 atggtggggc atcgctcggc aaacatgacg ctggaacagc ttgaatctct caatgcagag
    20941 cagaaagcgc gcacagtgtt ggtggtgcag gaccccttta ccagctatta cgatgcgcaa
    21001 gtggtggcgg attttgtccg tctggtcgaa aaattaggtt tccagcctgt gttactgcca
    21061 ttttcgccaa atggcaaagc ccagcatatt aaaggttttc ttaatcgttt tgcgaagacg
    21121 gcgaaaaaga cggcggattt cctcaaccgt atggcgaagc tgggtatgcc aatggtgggc
    21181 gtcgatccgg cgctggtact ttgttatcgc gatgaatata aactggcgct gggcgaggaa
    21241 cgtggcgagt ttaacgtctt actggcgaat gaatggctgg caagcgcact tgagtcacag
    21301 ccagtggcta cagtcagcgg tgaatcatgg tatttctttg gtcactgtac cgaagttacc
    21361 gccttgccgg gtgcgccagc acaatgggcc gcgatatttg cccgttttgg cgcgaaactg
    21421 gaaaatgtca gcgtgggttg ctgcggcatg gcagggactt acggacatga agcgaaaaac
    21481 cataaaaatt cgctcgggat ctatgagtta tcctggcatc aggctatgca gcgactgccg
    21541 cgtaaccgct gtctggcgac cggatattcc tgccgtagtc aggtcaaacg ggttgaaggc
    21601 acgggggtac gccatcctgt gcaggcttta ctggagatta ttaaatgata tggaaacgaa
    21661 aaatcaccct ggaagcactg aatgccatgg gtgaaggaaa catggtgggg ttactggata
    21721 ttcgctttga acatattggt gatgacaccc ttgaagcgac aatgccagta gactcgcgga
    21781 caaagcagcc tttcgggttg ctgcatggag gcgcatccgt ggtgctggcc gaaagtatcg
    21841 gttccgttgc cggttattta tgtaccgaag gtgagcaaaa agtggttggt ctggaaatca
    21901 atgctaatca cgtccgctcg gcacgagaag ggcgggtgcg cggcatatgt aaaccgttgc
    21961 atctcggttc gcgtcaccag gtctggcaga ttgaaatctt cgatgagaaa gggcgtttgt
    22021 gctgttcgtc acgattgacg accgccattt tgtgacgttg tcaccagaaa agtgtgacgc
    22081 atattgcttt tggtcattat ctgcacagta aacggtggta tactgtgcag gtcttgtcta
    22141 aaagcgagga tgctatgtct actcaacttg atcccaccca gctggccatt gaatttttac
    22201 gccgtgacca gagcaatctt tcacctgccc aatatctcaa acgtctgaaa caactagaac
    22261 ttgaatttgc tgacctgctt actctctctt ctgctgaatt aaaagaagaa atctactttg
    22321 cctggcgtct gggcgtgcat tgatcctgac ttaatgccgc aagttcgcca aatcgacctg
    22381 gattaacctc cgtccaggtt cgatgacctt cttaatactg cttatctacc gcctgatatt
    22441 gttcaaaaat aatactaatc gcagtttgtg ttaaaacggc gggttagctt tatgagatta
    22501 ttcttatcgc cccttcaaga gctaagccac tgagagtgcc ggagataagc gccggatggg
    22561 gtagaaaccc ttaagcctgt gtcgcacaga cttaagggtt ttcttatttc tgaacgctgc
    22621 acttttttac ctatctctta aatagtcatt ttcaatacaa ctttttcttt ttcatcacag
    22681 ccttaatgct ccttagataa taaccattat ctaacaatga gatacctaat tcttagcgtg
    22741 cctgttaacc cacacatcag ggtctatgct tattaaaagc aggcaaaaag catgttttac
    22801 ggtaaagccc ctgcgtttgc tgggttgaac tgataatcat tatcactaac atgctgttat
    22861 acgctgaaag cgatgaagtg aggtaaatcg atggacatgc attcaggaac ctttaaccca
    22921 caagatttcg cctggcaagg cttaacgctg acacccgcag cggcgataca catccgtgag
    22981 ctggtggcaa agcagccggg tatggtcggc gtgcgcttag gcgtgaagca aacgggctgc
    23041 gcgggctttg gctatgtgct cgacagtgtt agcgagccgg acaaagacga tctgctgttt
    23101 gaacacgacg gcgcgaagct gtttgtcccg ctgcaagcga tgccgtttat tgatggcacg
    23161 gaagtcgatt tcgttcgtga aggacttaat cagatattca aatttcacaa ccctaaagcc
    23221 cagaatgaat gtggctgtgg cgaaagcttt ggggtatagg cggtactatg tctcgtaata
    23281 ctgaagcaac tgacgatgtc aaaacctgga ccggcggccc gctgaattat aaagaaggat
    23341 tcttcaccca gttagccacc gatgagctgg caaaggggat aaacgaagag gtggtgcgcg
    23401 caatttcggc gaagcgtaat gagccggagt ggatgctgga gtttcgtcta aacgcctatc
    23461 gcgcatggct ggagatggaa gaaccgcact ggttgaaagc gcactacgac aagctgaatt
    23521 atcaggatta cagctactac tcagcaccat cgtgcggtaa ttgtgacgac acttgcgcgt
    23581 ctgaacctgg cgcggtgcag caaactggcg cgaacgcctt tttaagtaaa gaggtggagg
    23641 cggcgtttga gcagttgggc gttcccgtgc gggaaggcaa agaggtggcg gtggatgcca
    23701 ttttcgactc agtttcggtt gccactactt atcgcgaaaa actggcggag cagggaatta
    23761 ttttctgttc ctttggtgag gcgatccacg atcacccgga actggtgcgt aaatatctcg
    23821 gcaccgtggt gccggggaat gacaacttct ttgccgcgct taatgcggcg gtagcctctg
    23881 acggtacgtt tatttatgtg cctaaaggcg tgcgctgccc gatggaactt tccacctatt
    23941 ttcgcattaa cgcggaaaaa accgggcagt ttgagcgcac cattctggtg gccgacgaag
    24001 acagctacgt cagctacatt gaaggctgtt ccgctccggt gcgtgacagc tatcagttac
    24061 acgcggcagt ggtggaagtc atcatccata aaaacgccga ggtgaaatat tccacggtac
    24121 aaaactggtt ccctggcgat aacaacaccg gcggtattct caacttcgtc accaagcgtg
    24181 ctttgtgcga aggcgaaaac agcaaaatgt catggacgca atcagaaacc gggtcagcga
    24241 ttacgtggaa atatcccagc tgcattttgc gcggcgataa ctccattggt gagttttact
    24301 cagtggcgct gaccagcggt catcagcaag cggataccgg caccaagatg atccacatcg
    24361 gtaaaaacac caaatcgacc attatctcga aagggatctc tgccggacat agtcagaaca
    24421 gttatcgcgg cttagtgaaa atcatgccga cggcaaccaa tgcgcgcaat ttcactcagt
    24481 gcgactcaat gctgattggc gctaattgtg gggcgcatac cttcccgtat gtcgaatgtc
    24541 gcaataacag cgcacaactg gagcacgagg caacgacatc acgtattggt gaagatcaac
    24601 tgttttactg cctgcaacgc gggatcagcg aagaagacgc catctcgatg attgttaacg
    24661 gtttctgcaa agacgtgttc tcagaactgc cgctggaatt tgccgttgaa gcacaaaaac
    24721 tcctcgccat cagtcttgaa cacagcgtcg gataaggaat aaacatgtta agtattaaag
    24781 acttacacgt cagcgtggaa gataaagcta tcctgcgtgg attaagcctc gacgttcgtc
    24841 ccggcgaagt tcacgccatt atggggccaa acggttcggg caaaagtacc ttatcggcaa
    24901 cgcttgccgg gcgagaagat tatgaagtga cgggcggcac ggttgagttc aaaggcaaag
    24961 atttgcttgc gctgtcgccg gaagatcgtg caggcgaagg catctttatg gccttccagt
    25021 atccggtgga gattcccggc gtcagtaacc agtttttcct gcaaacggca cttaatgcgg
    25081 tgcgctgcta tcgcggccag gaaacgctcg accgctttga ttttcaggat ttgatggaag
    25141 agaaaatcgc tctcctgaag atgccggaag atttattaac ccgttcggta aacgttggtt
    25201 tttccggcgg cgagaaaaag cgcaacgata ttttgcaaat ggcggtgctg gaaccggagt
    25261 tatgcattct tgatgagtcg gactccgggc tggatattga cgcattaaaa gtggtcgccg
    25321 atggcgtgaa ctcgctgcgt gatggcaagc gctcattcat cattgttacg cactaccaac
    25381 gcattctcga ctacatcaag cctgattacg ttcatgtgct atatcaggga cgaattgtaa
    25441 aatccggcga tttcacgttg gtcaaacaac tggaggagca gggttatggc tggcttaccg
    25501 aacagcagta atgcgctgca acagtggcat cacttgtttg aagctgaagg gacaaaacgc
    25561 tcaccgcaag cacagcagca tttacaacaa ttgctgcgta ccggactgcc gacacgtaaa
    25621 catgaaaact ggaaatatac gccgctggaa gggctgacca atagccagtt tgtcagcatt
    25681 gcgggagaga tatccccaca gcagcgagat gccttagcgt taacgttaga cgctgtgcgg
    25741 ctggtatttg tcgatggacg ttacgtgtca gcactgagcg atgcgactga aggcagcggg
    25801 tatgaagtga gcattaacga cgaccgtcag ggggtacccg acgctattca ggcggaagtg
    25861 tttctgcatt tgacggaaag cctggcacaa agcgtgacgc atatcgccgt gaagcgcggt
    25921 caacggccgg caaagccatt gctgttaatg catattaccc agggcgtggc aggtgaagag
    25981 gtcaacactg cccattaccg acatcatctg gatctggcgg aaggtgccga agcaacggtg
    26041 atcgaacatt ttgtcagcct taatgatgct cgtcacttca ccggcgcacg gttcactatc
    26101 aatgtcgcag cgaacgccca cttgcagcat atcaagctgg cgtttgaaaa cccggtcagt
    26161 caccactttg cccataacga tttgttgctg gctgacgatg ccaccgcatt tagccatagt
    26221 ttcctgctgg gtggcgcagt gttacgacac aacaccagta cgcaactcaa tggcgaaaac
    26281 agcacgctgc ggatcaatag cctggcgatg ccggtgaaaa acgaggtgtg tgatacccgt
    26341 acctggctgg aacacaataa aggtttttgt aacagccgac agttgcacaa aactatcgtc
    26401 agcgacaaag gccgcgcggt atttaacggt ttgatcaacg tcgcgcagca cgccatcaaa
    26461 acggatggtc agatgaccaa caataatctg ctgatgggca aactggcaga agtggatacg
    26521 aaaccgcagc tggaaatcta tgcagatgat gtgaaatgca gccacggcgc gacggtgggg
    26581 cgtattgatg atgaacagat gttctatctg cgctcgcgcg ggatcaatca gcaggatgcc
    26641 cagcagatga tcatttacgc ctttgctgcc gaactgacgg aagcactgcg tgatgagggg
    26701 cttaaacagc aggtgctggc cagaatcggt caacggctgc caggaggtgc aagatgactt
    26761 tttccgtcga caaagtgcgg gccgactttc cggtgctttc tcgtgaggta aacggtttgc
    26821 cgctggctta tctcgacagc gccgccagtg cgcagaaacc gagccaggtg attgacgccg
    26881 aggccgagtt ttatcgtcat ggctacgcgg cggtgcatcg cggtattcat accttaagcg
    26941 cccaagcgac cgagaaaatg gagaacgtgc gcaagcgggc atcgctgttt attaatgccc
    27001 gttcggcgga agagctggtg ttcgtccgcg gcacgacgga agggatcaat ctggtcgcca
    27061 atagctgggg taacagcaat gtgcgggcgg gcgataacat catcatcagt cagatggagc
    27121 accacgctaa cattgttccc tggcagatgc tttgcgcacg cgttggcgca gagctgcgtg
    27181 tgatcccgct caatcccgac ggtacgttgc aactggagac gctgcctacg ctgtttgatg
    27241 agaaaactcg cctgctggca attactcatg tctccaacgt gcttggcaca gaaaatccac
    27301 tggcggaaat gatcacgctt gcgcaccagc atggcgcaaa agtgctggtg gatggcgctc
    27361 aggcggtgat gcatcatccg gtggatgttc aggcgctgga ttgcgacttt tacgtgttct
    27421 ccgggcataa actgtatggc cccaccggaa ttggcattct ttatgtgaaa gaagccttgt
    27481 tgcaggagat gccgccgtgg gaagggggcg gttctatgat cgccaccgtc agcctgagtg
    27541 aaggcacaac ctggaccaaa gcaccttggc ggtttgaagc cggtacaccc aataccgggg
    27601 gcatcattgg tcttggcgcg gcgctggagt atgtttcggc gctggggctt aataacatag
    27661 ccgagtatga acagaatctg atgcactacg cgctatcaca gctggaatct gtaccggatc
    27721 tcactctcta tggtccgcag aacaggcttg gcgttattgc ttttaatctc ggtaaacacc
    27781 atgcctatga tgttggcagt tttctcgata attacggcat tgctgtgcgt accggacatc
    27841 actgcgctat gccattaatg gcctattaca acgtccctgc gatgtgtcgg gcgtcgctgg
    27901 ccatgtataa cacccatgaa gaagtggatc gtctggtgac cggcctgcaa cgtattcacc
    27961 gtctgctggg ataacaggga ggcactatgg ctgtattgcc ggataaagaa aaattgctgc
    28021 gtaatttttt acgctgcgcc aactgggaag agaaatatct ctacattatt gagctgggcc
    28081 agcgtctgcc agaattacgc gacgaagaca gaagcccaca aaatagcatt cagggctgcc
    28141 agagtcaggt gtggattgtc atgcgccaga atgcgcaggg aattattgaa ttgcagggcg
    28201 acagcgatgc ggcgattgtg aaagggctta ttgcggtcgt ctttattctc tacgatcaga
    28261 tgacgccgca ggatattgtc aatttcgatg tgcgtccgtg gtttgaaaaa atggcgctca
    28321 cccaacatct caccccatct cgttcacaag gtctggaagc gatgattcgc gcaattcgcg
    28381 ccaaagccgc tgcacttagc taaactaaag ggacagcttt catccggttg gctcacattg
    28441 ccggatggtc aatgtttaca ctgacctgca caggagtgtt tcggtttcag gtgaacataa
    28501 gcgccatccg acgtttcagc gtgagtctcc ggcaaataca ggaggtttac aatgaaacgc
    28561 gcgtctttgc ttacactcac gcttatcggc gcttttagcg ccatccaggc tgcctgggcg
    28621 gttgattatc cgctaccacc aaccggaagc cgactggttg ggcaaaatca aacgtatacg
    28681 gtgcaggaag gggataaaaa ccttcaggcc atcgcccgac gttttgatac tgcggcaatg
    28741 ttgatccttg aagccaataa cactatcgcc ccggtgccaa aacccggtac gacgataact
    28801 attccttcgc aactgttatt acctgatgcg ccgcgtcagg ggattatcgt taaccttgca
    28861 gagctgcgcc tttattatta tccgccggga gaaaatatcg tgcaggttta tccgataggt
    28921 attggcttgc aggggctgga aacgccggtg atggaaacgc gtgtggggca gaaaatccct
    28981 aacccaacct ggacgcctac tgcaggcatt cgtcaacgtt cgctggagcg tggtattaaa
    29041 ttaccgccag tcgttcctgc cggaccaaat aacccgctag gacgttacgc actgcgcctc
    29101 gcgcatggta atggcgaata cctcattcat ggcaccagtg cgccggacag cgtcggtttg
    29161 cgcgtcagtt cagggtgtat tcgcatgaat gcaccggata ttaaagcctt gttctccagc
    29221 gtgcggacgg gaacgccggt gaaagtgatc aacgaacctg tgaaatattc cgtggagcct
    29281 aacgggatgc gttatgttga agtacatcga ccactatcgg cagaagaaca gcagaacgtt
    29341 cagacaatgc catacacact gccagcaggc tttacgcaat ttaaagacaa taaggctgta
    29401 gatcagaagt tagtcgataa agcgttgtat cgtcgggcag ggtatccggt tgcggtgagc
    29461 agtggagcaa ctcccacagc cagcaatgcg ccttcagtag agtcagcgca gaatggtgaa
    29521 ccagagcaag ggaatatgtt acgcgcgacg cagtagcggt aaacggcaga caaaaaaaat
    29581 ggcgcacaat gtgcgccatt tttcacttca caggtactat tacttgcggt atttagtagc
    29641 catgttgtcc agacgctggt tagcacgagc tgcgtcatct ttagcagcct gaacgtcgga
    29701 acgcattgcg ttcacgtcgt tgctcagctg gtcaacttta gcgttcagag tctgaacgtc
    29761 agaagacagc tgatcgattt tagcgttgct ggagcaacct gccagcagag tagaacccag
    29821 gattaccgcg cccagtacca gtttagtagc tttcattatt aataccctct agattgagtt
    29881 aatctccatg tagcgttaca agtattacac aaactttttt atgttgagaa tatttttttg
    29941 atgggaaggc acttattttt gatcgttcgc tcaaagaagc atcgaacgct gggttttaga
    30001 taaaaaaatt gagagaaaac aggatgcttc catcggattc atcttagata aagtgagatt
    30061 atatagtgaa atccgatttg attttttatt gcttctggtt atcgattaaa taaaaaaagc
    30121 gcccatcagg gcgcttcgat atacaaatta attcacaaaa gcaatattac aggacgtgaa
    30181 cagatgcggt gttagtagtg ccgctcggta ccagtgcacc agaaaccata actacaacgt
    30241 cacctttgtg tgccagaccg ctctgcagag ccagttcttt acccagacgg tagaaatcat
    30301 cagtagaagt gatctcttta accagctgcg gcacaacgcc tttgctcagt accaactgat
    30361 gagccgtttt ttcgttggtg gtcagtgcca ggatggtggc atccgggaag tatttacgta
    30421 ctgcgcgagc agatttaccg ccctgggtag caaccacgat cagcggagca tccagttttt
    30481 cagcagtttc aacggcacca cggcataccg cttcggtaat gcgcagttta cggttgtcat
    30541 tgttgaactc gagacggctg ttcatcacgc ggtcggtacg ttcgcagatg gtcgccatga
    30601 tagaaaccgc ttccagcggg tatttacctt ttgcggattc accagacagc atcactgcgt
    30661 cagtaccgtc gaggatggcg tttgcaacgt caccggcttc tgcgcgagtc gggcgtgggt
    30721 ttttgatcat ggaatccagc atctgggtcg cagtgataac gactttacgt gcacggatac
    30781 atttttcgat catcatcttc tgggcgaaga taacttcttc taccgggatt tctacaccca
    30841 ggtcgccacg cgcaaccatg atgccgtcag aggcttcgag gatttcgtcg aagttgttga
    30901 ggccttcctg gttttcgatt ttggagatga tgtggatgtt ttcgccgccg tgcgctttca
    30961 ggtgctcacg gatttcgata acgtcagaac gcttacgaat aaaggaagca gcaacaaagt
    31021 ctacgccttg ttcgcaacca aagatcaggt cctgtttgtc tttttcagcc agtgctggca
    31081 gagcaatgga aacgccaggc aggttcacac ctttgttttc gcccaggtcg ccgttgttca
    31141 gtactttaca gataactttg ttaccttcaa tggcggtaac ttccataccg atcagaccat
    31201 cgtcaaccag tacggtgttg ccaacagaca ggtcagtagt gaaaccttca tacgttaccg
    31261 caaccatttc gctgttgccg ataacagatt tatcagtggt gaaagtaaag gtctgaccag
    31321 ctttcagaga aacgtcgtta ccgccttcca gtttcatggt gcggatttcc ggacctttgg
    31381 tatcaagcag gatagcggcg gttttaccag ttttgctcat cacgttgcgc agattctgaa
    31441 tgcgctgacc gtgttctgca tagtcaccat gagagaagtt cagacgcata acgttcatgc
    31501 cagcgtccag cattttagct aacatctctt cagattcggt tttcggtccg atggtgcaaa
    31561 caattttggt ctttttcatg acagtcttag tctttaagtt gagaaggatg ggagaaactt
    31621 gctttctggg cgcactgccg gaaagcgaat ccggtattac gaaagtggtg gtgcctcgct
    31681 ctaaggatag gtgacatcga aaaagcgtgc agaggaatgt gtgcttgtgt ctcagcccaa
    31741 cgtgagtgcc tgtttttgag tcgttgagtt ctacagtcca aagtgctgaa accattcaag
    31801 agtcaattgg cgcgcattat acgctgaaat taatccaaaa ggaaaacaaa aacagcgttt
    31861 caaaaaatat acaacttgtt agacaataaa ttgttatcaa agatttaacg ttccagggaa
    31921 aaggttacgc aaaaacttca ctgtttgcgc aacgtctggt gccgaagttg tcagtgtgag
    31981 gataagtgaa agttcatccg gtgtataaat atatcatcgc tcaattgcta cagttaaaat
    32041 aatttatcgc gttatttgtc tgattttgtt tgatttttgt ttaattggtt agattgatat
    32101 atgctgatag ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg
    32161 aactgtaccg agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg
    32221 ggcaggaacc aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac
    32281 gcattcagcg cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa
    32341 aatgaccatt caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc
    32401 cagttaatca ttctaaataa caactgctga aattatacga atttcggtat gtattttaat
    32461 ttatagcgat aatgttattt ttattttctg caatgaacgt cacaaataat tgtcgcttaa
    32521 gttaaattta tttcaaatga taataaatat cag
//
LOCUS       DAZKIV010000035        32535 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZKIV010000035 DAZKIV010000000
VERSION     DAZKIV010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.035"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            89..1378
                     /gene="hpaG"
                     /locus_tag="ACHOT6_001671"
     CDS             89..1378
                     /gene="hpaG"
                     /locus_tag="ACHOT6_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFP9946906.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1375..2841
                     /gene="hpaE"
                     /locus_tag="ACHOT6_001672"
     CDS             1375..2841
                     /gene="hpaE"
                     /locus_tag="ACHOT6_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9946907.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2843..3694
                     /gene="hpaD"
                     /locus_tag="ACHOT6_001673"
     CDS             2843..3694
                     /gene="hpaD"
                     /locus_tag="ACHOT6_001673"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFP9946908.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGIYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3704..4084
                     /locus_tag="ACHOT6_001674"
     CDS             3704..4084
                     /locus_tag="ACHOT6_001674"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFP9946909.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            4152..4955
                     /gene="hpaH"
                     /locus_tag="ACHOT6_001675"
     CDS             4152..4955
                     /gene="hpaH"
                     /locus_tag="ACHOT6_001675"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFP9946910.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4966..5754
                     /gene="hpaI"
                     /locus_tag="ACHOT6_001676"
     CDS             4966..5754
                     /gene="hpaI"
                     /locus_tag="ACHOT6_001676"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFP9946911.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5929..7305
                     /gene="hpaX"
                     /locus_tag="ACHOT6_001677"
     CDS             5929..7305
                     /gene="hpaX"
                     /locus_tag="ACHOT6_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFP9946912.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7315..8205
                     /gene="hpaA"
                     /locus_tag="ACHOT6_001678"
     CDS             7315..8205
                     /gene="hpaA"
                     /locus_tag="ACHOT6_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFP9946913.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LSWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8456..10018
                     /gene="hpaB"
                     /locus_tag="ACHOT6_001679"
     CDS             8456..10018
                     /gene="hpaB"
                     /locus_tag="ACHOT6_001679"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFP9946914.1"
                     /translation="MKPEDFRASPQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10036..10548
                     /locus_tag="ACHOT6_001680"
     CDS             10036..10548
                     /locus_tag="ACHOT6_001680"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFP9946915.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10941..13091
                     /gene="btsT"
                     /locus_tag="ACHOT6_001681"
     CDS             10941..13091
                     /gene="btsT"
                     /locus_tag="ACHOT6_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFP9946916.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13141..13344
                     /gene="yjiX"
                     /locus_tag="ACHOT6_001682"
     CDS             13141..13344
                     /gene="yjiX"
                     /locus_tag="ACHOT6_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9946917.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13355..14311
                     /gene="yjiA"
                     /locus_tag="ACHOT6_001683"
     CDS             13355..14311
                     /gene="yjiA"
                     /locus_tag="ACHOT6_001683"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFP9946918.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14389..16928)
                     /locus_tag="ACHOT6_001684"
                     /pseudo
     CDS             complement(14389..16928)
                     /locus_tag="ACHOT6_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
     gene            complement(17280..17444)
                     /gene="yjiS"
                     /locus_tag="ACHOT6_001685"
     CDS             complement(17280..17444)
                     /gene="yjiS"
                     /locus_tag="ACHOT6_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFP9946919.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLSREDVE"
     gene            17621..19033
                     /gene="yjiR"
                     /locus_tag="ACHOT6_001686"
     CDS             17621..19033
                     /gene="yjiR"
                     /locus_tag="ACHOT6_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9946920.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLVAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWVELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(19275..20231)
                     /locus_tag="ACHOT6_001687"
     CDS             complement(19275..20231)
                     /locus_tag="ACHOT6_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFP9946921.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGRRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKRDPLIEY"
     gene            20698..21930
                     /gene="mdtM"
                     /locus_tag="ACHOT6_001688"
     CDS             20698..21930
                     /gene="mdtM"
                     /locus_tag="ACHOT6_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFP9946922.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVLRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGSVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21971..23251
                     /gene="yjiN"
                     /locus_tag="ACHOT6_001689"
     CDS             21971..23251
                     /gene="yjiN"
                     /locus_tag="ACHOT6_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFP9946923.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDT
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            23367..24518
                     /gene="yjiM"
                     /locus_tag="ACHOT6_001690"
     CDS             23367..24518
                     /gene="yjiM"
                     /locus_tag="ACHOT6_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFP9946924.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24528..25295
                     /gene="yjiL"
                     /locus_tag="ACHOT6_001691"
     CDS             24528..25295
                     /gene="yjiL"
                     /locus_tag="ACHOT6_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFP9946925.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25292..25549
                     /gene="ytiA"
                     /locus_tag="ACHOT6_001692"
     CDS             25292..25549
                     /gene="ytiA"
                     /locus_tag="ACHOT6_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFP9946926.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            25614..26474
                     /gene="yjiK"
                     /locus_tag="ACHOT6_001693"
     CDS             25614..26474
                     /gene="yjiK"
                     /locus_tag="ACHOT6_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9946927.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26542..27720
                     /gene="yjiJ"
                     /locus_tag="ACHOT6_001694"
     CDS             26542..27720
                     /gene="yjiJ"
                     /locus_tag="ACHOT6_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946928.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFALSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFSWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27733..28287)
                     /gene="kptA"
                     /locus_tag="ACHOT6_001695"
     CDS             complement(27733..28287)
                     /gene="kptA"
                     /locus_tag="ACHOT6_001695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFP9946929.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28537..29219
                     /gene="yjiH"
                     /locus_tag="ACHOT6_001696"
                     /pseudo
     CDS             28537..29219
                     /gene="yjiH"
                     /locus_tag="ACHOT6_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
     gene            29216..29677
                     /gene="yjiG"
                     /locus_tag="ACHOT6_001697"
     CDS             29216..29677
                     /gene="yjiG"
                     /locus_tag="ACHOT6_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFP9946930.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29690..30862
                     /gene="iadA"
                     /locus_tag="ACHOT6_001698"
     CDS             29690..30862
                     /gene="iadA"
                     /locus_tag="ACHOT6_001698"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9946931.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30927..31838
                     /gene="hypT"
                     /locus_tag="ACHOT6_001699"
                     /gene_synonym="qseD"
     CDS             30927..31838
                     /gene="hypT"
                     /locus_tag="ACHOT6_001699"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFP9946932.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSLAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31831..32223)
                     /gene="iraD"
                     /locus_tag="ACHOT6_001700"
     CDS             complement(31831..32223)
                     /gene="iraD"
                     /locus_tag="ACHOT6_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFP9946933.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDILTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHTLAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(32220..32303)
                     /gene="idlP"
                     /locus_tag="ACHOT6_001701"
     CDS             complement(32220..32303)
                     /gene="idlP"
                     /locus_tag="ACHOT6_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFP9946934.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(32419..>32535)
                     /gene="ytiD"
                     /locus_tag="ACHOT6_001702"
     CDS             complement(32419..>32535)
                     /gene="ytiD"
                     /locus_tag="ACHOT6_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtiD"
                     /protein_id="HFP9946935.1"
                     /translation="FPPELSSGGDKYFHLRNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         7479 a         8598 c         8521 g         7937 t
ORIGIN      
        1 tcacttactt ttgccaaact acgcaatcgg gaataaaagt aatcattaac atattaatga
       61 ttaaaatgat cgaatccgag gagtggtaat gaaaggcact atcttcgccg tagcgttgaa
      121 ccatcgcagc caacttgatg catggcagga agcgttccag caatccccct acaaagcccc
      181 gcctaaaact gcggtctggt ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc
      241 gattcccttt ccacagggtg aaaaggtact gagcggtgcg actgttgcgc tgattgtggg
      301 aaaaacggcg acgaaagtac gtgaagaaga tgcggcagag tacatcgccg gatatgcgct
      361 ggctaacgac gtcagcctgc cggaagagag cttttaccgc ccggcaatca aagcaaaatg
      421 tcgtgatgga ttctgcccca ttggcgaaac cgtggctctc agcaatgtcg ataatctgac
      481 catctatacc gagatcaacg ggcgtcctgc cgatcactgg aataccgccg atttacaacg
      541 taacgccgcg cagttgctga gcgccctgag cgaatttgcc acgctgaatc caggcgacgc
      601 cattctgctc ggcacgccac aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt
      661 tctcgcagaa ggtttcccgc cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac
      721 gcgcaagagc ttcccaacgc agccacaccc gcacggcacg ctgtttgccc tcggcctgaa
      781 ctacgccgac cacgccagcg aactggaatt taagccaccg gaagaaccgc tggtgttcct
      841 gaaagcgcca aataccctca ctggcgataa ccagacctcc gtgcgtccaa acaatattga
      901 atacatgcac tatgaagcgg agctggtggt agttattggc aaacaggcgc gtaacgtcag
      961 cgaagccgat gccatggatt atgtcgcggg ctacaccgtg tgtaacgact acgccattcg
     1021 cgactatctg gaaaactact accgccctaa cctgcgggtc aaaagccgcg acggactgac
     1081 gccgatgctt tcaaccatcg tgccgaaaga ggcgatcccg gacccgcata atctgaccct
     1141 tcgcaccttc gtcaacggcg agttacgcca gcaaggcacc accgccgatc tgatcttcag
     1201 cgtgcccttc ctgatcgcct acttaagcga atttatgacc ctgaatccgg gcgacatgat
     1261 cgccaccggc acaccaaaag gcttatctga cgtggtgcct ggcgatgaag tggtggtgga
     1321 agtagaaggc gtgggccgcc tggtgaaccg aattgtgagt gaggaaacag cgaaatgaaa
     1381 aaagtaaatc attggatcaa cggtaaaaat gttgcaggta acgactactt ccagactacc
     1441 aatccggcaa cgggtgaagt gctggcggat gtggcctccg gcggtgaagc ggagatcaat
     1501 caggcggtag cggcagcgaa agaggcgttc ccgaaatggg ccaatctgcc gatgaaagag
     1561 cgcgcgcgcc tgatgcgccg tctgggcgat ctgatcgacc agaacgtgcc ggagatcgcc
     1621 gcgatggaaa ccgcggacac gggcctgccg atccatcaga ccaaaaatgt gttgatccca
     1681 cgcgcttctc acaactttga atttttcgcg gaagtctgcc agcagatgaa cggcaagact
     1741 tatccggtcg acgacaagat gctcaactac acgctggtgc agccggtagg cgtttgtgca
     1801 ctggtgtcac cgtggaacgt gccgtttatg accgccacct ggaaggtcgc gccgtgtctg
     1861 gcgctgggca ataccgcggt actgaaaatg tcggaactct ccccgctgac cgccgaccgc
     1921 ctgggtgagc tggcgctgga agctggtatt ccggcgggcg tgctgaacgt ggtacagggc
     1981 tacggcgcaa ccgcaggcga tgcgctggtc cgtcatcatg acgtgcgtgc cgtgtcgttc
     2041 accggcggta cggcgaccgg gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc
     2101 atggaactgg gcggtaaatc gccggtgctg atttttgaag atgctgatat tgaacgcgcg
     2161 ctggacgccg ccctgttcac catcttctcg atcaacggcg aacgctgcac cgccggttcg
     2221 cgcatcttta ttcagcaaag catctacccg gaattcgtta aacgctttgc cgaacgcgcc
     2281 aaccgtctgc gcgtgggcga tccgaacgat ccgaataccc aggttggcgc gcttatcagc
     2341 cagcagcact gggaaaaagt ctccggctat atccgtctcg gcattgaaga aggcgcaacc
     2401 ctgctggcgg gcggcccgga taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac
     2461 ttcctgcgcc caaccgtgct ggcagacgtt gataaccgta tgcgcgtcgc ccaggaagag
     2521 attttcgggc cggtcgcctg cctgctgccg tttaaagacg aagctgaagg cttacgcctg
     2581 gcaaacgacg tggagtacgg cctcgcgtcg tacatctgga ctcaggatgt cagcaaagtg
     2641 ctgcgtctgg cgcgcgggat tgaagcgggc atggtgttcg tcaacaccca gaacgtgcgt
     2701 gatttgcgcc agccgtttgg cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag
     2761 tacagcttcg aagtgttcgc ggaaatgaag aacgtctgca tttccatggg cgaccatcca
     2821 attccgaaat ggggagtctg atatgggtaa gttagcgtta gccgccaaga tcacgcacgt
     2881 accgtcgatg tatctctctg aactgccagg gaaaaaccac ggttgccgcc agggcgcgat
     2941 cgacgggcat aaagagatca gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt
     3001 tttcgatacc cactggctgg tcaacagtgc ttatcacatc aactgtgcag accattttga
     3061 aggcatctac accagcaacg agctgccgca ttttatccgc gatatgacct acaactacga
     3121 gggcaacccg gagttggggc agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc
     3181 aaaagcgcac aacattccca gcctgaaact ggaatacggc acgctggtac cgatgcgcta
     3241 catgaatgaa gacaagcact tcaaagtggt ctccatttcg gctttctgca cggttcacga
     3301 ttttgctgac agccgcaagc tgggcgaagc gattctgaaa gcgatcgaac agtacgacgg
     3361 caccgtggcg gtccttgcca gcggttcgtt atcgcaccgc tttattgacg atcagcgtgc
     3421 agaagaaggg atgaacagct acacccgcga gttcgaccgc cagatggacg agcgcgtggt
     3481 gaagttgtgg cgcgaaggcc agttcaaaga gttctgcaat atgctgccgg agtacgccga
     3541 ctactgctac ggcgaaggca atatgcacga cacggtgatg ctgctgggga tgctcggctg
     3601 ggataaatac gacggcaagg tggagtttat caccgagcta ttcccaagct ctggcaccgg
     3661 tcaggttaac gctgttttcc cgcttcccgc gtaaggaacc accatgccgc actttatcgt
     3721 ggaatgtact gaaaatattc gtgaagaagc ccgcttacct gagctgtttg ccagtgttaa
     3781 cactgcactg gcggcaacgg ggatttttcc gcttggggga atacgtagcc gggcgcactg
     3841 gatcgatacc tggcaaatgg ctgatggcaa gcacgattac gcctttgtgc atatgacgct
     3901 aaaaatcggc agcgggcgca gcctggaaag ccgtcaggaa gtgggcgaaa tgctgtttga
     3961 tctgattaaa acgcacttcg cctcactgat ggagagccgt tacctggcgc tttcatttga
     4021 gattgcagag ctgcacccga cgctgaattt taaacaaaac aacgtgcacg cgttgtttaa
     4081 ataaatttgc accagcttcc aggccggatg ataaggcgca gccgcatccg gcaatgccac
     4141 aggatatcgc tatgttcgat aaacacaccc acaccctgat cgcccagcgt ctggatcagg
     4201 cagaaaaaca gcgcgaacag atccgcgcga tctcgctgga ttacccggag atcaccatcg
     4261 aagacgctta cgcggtgcag cgtgaatggg ttcgactgaa aatcgccgaa ggtcgcacgc
     4321 tgaaaggcca caaaatcggc ctgacctcga aagcgatgca ggccagctca caaatcagcg
     4381 agccggatta cggtgccctg ctggatgaca tgttcttcca cgatggcagc gatatcccga
     4441 ccgatcgctt tatcgtgccg cgcattgaag tggagctggc tttcgtgctg gcaaaaccgc
     4501 tgcgtgggcc aaactgcacg ctgttcgacg tctacaacgc cacggactat gtgatcccgg
     4561 cgctggagct gatcgacgct cgctgccaca acatcgatcc ggaaacccag cgcccgcgta
     4621 aagtgttcga caccatttct gataacgccg ccaatgccgg ggtgatcctc ggtggtcgtc
     4681 ccattaagcc cgatgagttg gatctacgtt ggatctccgc cctgatgtat cgcaatggcg
     4741 tgattgaaga aaccggcgtc gccgctggtg tgctgaatca tccggcaaac ggcgtggcct
     4801 ggctggcgaa caaactcgca ccgtatgacg tacaactgga agccgggcaa atcattctcg
     4861 gcggttcgtt cacccgcccg gttccggcgc gtaagggcga caccttccac gtcgattacg
     4921 gcaacatggg ctccattagc tgccgctttg tttaaggaga gaacgatgga aaacagtttt
     4981 aaagcggcgc tgaaagcagg ccgcccgcag atcggattat ggctggggct gagtagcagc
     5041 tacagcgcag agttactggc cggagcagga ttcgactggt tattgatcga cggtgagcac
     5101 gcgccgaata acgtgcaaac cgtgctcacc cagctacagg cgattgcgcc ctaccccagc
     5161 cagccggtgg tacgtccgtc gtggaacgat ccggtgcaaa tcaaacaact gctggacgtc
     5221 ggcacacaaa ccttgctggt gccgatggta caaaacgctg acgaagcccg tgaagcggta
     5281 cgcgccaccc gttatccccc cgccggtatt cgcggtgtgg gcagtgcgct ggcccgagcc
     5341 tcgcgctgga atcgtattcc tgattacctg caaaaagcca acgatcaaat gtgcgtgctg
     5401 gtgcagatcg aaacgcgtga ggcaatgaag aacttaccgc agattctgga cgtggaaggg
     5461 gtcgacggcg tgtttatcgg cccggcggat ctgagcgccg atatgggtta tgccggtaat
     5521 ccacagcacc cggaagtaca ggccgcgatt gagcaggcga tcgtgcagat ccgcgaagcg
     5581 ggcaaagcgc cggggatcct gatcgccaat gagcaactgg caaaacgcta tctggaactg
     5641 ggcgcgctgt ttgtcgccgt cggcgttgac accaccctgc tcgcccgcgc cgctgaagcg
     5701 ctggcagcac ggtttggcgc acaggccacg accgttaagc ccggcgtgta ttaatgccgg
     5761 atgcgctgca cttatccggc ctacactcgc accaaaccgt aggccggata aggcgtttac
     5821 gccgcatccg gcaaaaagcc gtaccaaatc gcggaccgga taaggcgttt gtgcgcatcc
     5881 gccataaacc ttgtcgtacc ctacaaaaat cccattagag gaagaaaaat gagcgacacc
     5941 tcacctgcca taccggagag tatcgatccg gcgaatcagc ataaagcgct gactgccgga
     6001 caacaggcgg ttattaagaa gctgtttcgc cgcctgatcg tctttctgtt cgtgctgttt
     6061 atcttctcgt tccttgatcg catcaacatc ggctttgccg gactcacgat gggacgcgac
     6121 ctcggtctga gcgccaccat gtttggcctc gctaccaccc tgttctatgc cgcttatgtc
     6181 atcttcggca ttcccagcaa cattatgctg agtattgtcg gtgcgcggcg ctggatcgcc
     6241 accatcatgg tgctttgggg catcgcctct actgccacca tgtttgccac tggccccacc
     6301 agcttgtacg tactgcgtat actggttggc attaccgaag ccggctttct gcccggcatt
     6361 ctgctgtatt taaccttctg gtttccggcc tatttccgcg cccgtgccaa cgccttgttt
     6421 atggtagcaa tgccggtaac gacagcgttg ggatcgatcg tttccggcta cattttgtcg
     6481 ctggatggcg taatggcatt aaaaggctgg cagtggctgt ttttgctgga aggcttcccg
     6541 tcggtattac tcggcgtcat ggtgtggttc tggcttgatg actcaccgga caaagctaag
     6601 tggctgacga aagaagacaa aaaatgcctg caagagatga tggataacga tcgtctgacg
     6661 ctggttcagc cagagggagc catcagccac cacgccatgc aacaacgcag catgtggcgg
     6721 gagatcttca ctccggtggt gatgatgtat accctggcgt atttctgcct gaccaacaca
     6781 cttagtgcga tcagcatctg gacaccgcag atcctacaaa gctttaatca gggcagcagt
     6841 aatatcacca tcggcctgct ggccgccgta ccgcagattt gtaccattct cgggatggtc
     6901 tactggagcc gtcactcaga tcgccgccag gaacgaaggc atcacaccgc ccttccttat
     6961 ttgttcgctg ccgctggttg gttactggct tcggcaactg atcacaacat gatccagatg
     7021 ctggggatca ttatggcttc gaccggatca ttcagcgcaa tggcgatttt ctggacaaca
     7081 ccggatcagt ccatcagcct gcgggcacga gcgatcggta ttgcggtgat caacgccact
     7141 ggcaacattg gttcagcgtt aagtccgttt atgatcggct ggttgaaaga tttgaccggc
     7201 agctttaaca gtggattgtg gtttgttgcc gcgctgctgg tgattggtgc ggggattatc
     7261 tgggcgattc caatgcagtc ctcccgtccg cgagcgaccc cgtaaggaac gacgatgtgt
     7321 gaccgtcaga ttgccaatat tgatatcagc aaagagtacg atgaaagcct gggcacggac
     7381 gatgtgcatt atcagtcgtt cgcccgcatg gcggcatttt ttggccgcca tatgctgcca
     7441 catcgccacg aacagtactt tcagatgcat ttcctcaata gcggacagat tgagctacag
     7501 cttgacgatc atcgctactc ggtggaagcg cccctgtttg tcctgacgcc gccgtcagta
     7561 cctcatgcgt ttattacgga gtctgacgcc gacggtcatg tgttgacggt acgggaagat
     7621 ctgagctggc ccctgctgga agttctttat ccgggcactc gggaaacctt cggcctgccg
     7681 gggatttgcc tgtcactggc agataaaccc gacgaactgg cggcgctgga acactattgg
     7741 caactgatag agcgggaatc ggtagaacaa ctgcctggac gggaacacac cctgacgtta
     7801 ctggcacagg cagtgttcac cctactgctg cgtaacgcaa aactcgacga ccatgccgcc
     7861 agcggaatgc gcggagaatt aaaactgttc cagcgtttta atatgctgat tgaaagccat
     7921 tttcatcagc actggacagt accggattac gctaacgaac tgcatatcac cgaatcacgc
     7981 ctcacggaca tctgccgccg ctttgccaac cgtccgccaa aacggttgat tttcgacagg
     8041 cagctgcgag aagccaagcg gctgctgctg ttttctgata acgccgtgaa caatattgcc
     8101 tggcaactcg gttttaagga tccagcttat tttgcgcgct tttttaatcg cttagtcggt
     8161 tgctcgccca gtgcttatcg tgccaaaaaa gtacctgtga cgtgaaaatc ccttagtctt
     8221 aacgggaaac caggcaccac ctgctattcc cctttctccg cctggagcaa ggggatttag
     8281 taacgtcaaa aatcgctaaa agcgacctcg atcacaaaac cgtacccgac ctgaaaagta
     8341 ccagccattg tcccaaaagt gtcttcccgt aatcgcccct gcaagcttca attgaataac
     8401 aaaaacaaca cacaaaatta acaatcgtat tccgattaat actgtagagg tcgacatgaa
     8461 accagaagat ttccgcgcca gtccccaacg tccgttcacc ggggaagagt atctgaaaag
     8521 cctgcaggat ggtcgcgaga tctatatcta tggcgagcga gtgaaagacg tcaccactca
     8581 tccggcattt cgtaatgcgg cagcgtctgt tgcccagctg tacgacgcac tgcacaaacc
     8641 ggagatgcag gactctctgt gctggaacac cgacaccggc agcggcggct atacccataa
     8701 attcttccgc gtggcgaaaa gtgccgacga cctgcgccag caacgcgacg ccatcgctga
     8761 gtggtcacgc ctgagctatg gctggatggg ccgtacccca gactacaaag ctgctttcgg
     8821 ttgcgcactg ggcgcgaatc cgggctttta cggtcagttc gagcagaacg cccgtaactg
     8881 gtacacccgt attcaggaaa ctggcctcta ctttaaccac gcgattgtta acccaccgat
     8941 cgatcgtcat ttgccgaccg ataaagtaaa agacgtttac atcaagctgg aaaaagagac
     9001 tgacgccggg attatcgtca gcggtgcgaa agtggttgcc accaactcgg cgctgactca
     9061 ctacaacatg attggcttcg gctcggcaca agtaatgggc gaaaacccgg acttcgcact
     9121 gatgttcgtt gcgccaatgg atgccgatgg cgtcaaatta atctcccgcg cctcttatga
     9181 gatggtcgcg ggtgctaccg gctcaccgta tgactacccg ctctccagcc gcttcgatga
     9241 gaacgatgcg attctggtga tggataacgt gctgatccca tgggaaaacg tgctgatcta
     9301 ccgcgatttt gatcgctgcc gtcgctggac gatggaaggc ggtttcgccc gtatgtatcc
     9361 gctgcaagcc tgtgtgcgcc tggcagtgaa actcgacttc attacggcac tgctgaaaaa
     9421 atcactcgaa tgtaccggca ccctggagtt ccgtggtgtg caggccgatc tcggtgaagt
     9481 ggtggcgtgg cgcaacacct tctgggcatt gagtgactcg atgtgttctg aagcgacgcc
     9541 gtgggtcaac ggggcttatt taccggatca tgccgcactg caaacctatc gcgtactggc
     9601 accaatggcc tacgcgaaga tcaaaaacat tatcgaacgc aacgttacca gtggcctgat
     9661 ctatctccct tccagtgccc gtgacctgaa caatccgcag atcgaccagt atctggcgaa
     9721 gtatgtgcgc ggttcgaacg gtatggatca cgtccagcgc atcaagatcc tcaaactgat
     9781 gtgggatgcc attggcagcg agtttggtgg tcgtcacgaa ctgtatgaaa tcaactactc
     9841 cggtagccag gatgagattc gcctgcagtg tctgcgccag gcacaaagct ccggcaatat
     9901 ggacaagatg atggcgatgg ttgatcgctg cctgtcggaa tacgaccaga acggctggac
     9961 tgtgcctcac ctgcacaaca acgacgatat caacatgctg gataagctgc tgaaataacg
    10021 cagcaggagg ttaagatgca attagatgaa caacgcctgc gctttcgtga cgcgatggcc
    10081 agcctgtcgg cagcggtaaa tattatcacc accgagggcg acgccggaca atgcgggatt
    10141 acggcaacgg ccgtctgctc ggtcacggat acaccaccat cgctgatggt gtgcattaac
    10201 gccaacagtg cgatgaaccc ggtttttcag ggcaacggca agttgtgcgt caacgtcctc
    10261 aaccatgagc aggaactgat ggcacgccac ttcgcgggca tgacgggcat ggcgatggaa
    10321 gagcgtttta gcctctcatg ctggcaaaaa ggtccgctgg cgcagccggt gctaaaaggt
    10381 tcgctggcca gtcttgaagg tgagatccgc gatgtgcagg caattggcac acatctggtg
    10441 tatctggtgg agattaaaaa catcatcctc agtgcagaag gtcacggact tatctacttt
    10501 aaacgccgtt tccatccggt gatgctggaa atggaagctg cgatttaagc tccagcccct
    10561 tcacgcattc gtcgaagggg catttttatt gtgatctcaa tcacatatcc acccgatgaa
    10621 taattcttgc ccaaccgctc accgccattc ctgaccgctt acatccctaa aataaccact
    10681 cagttattta ccttacttta cgcgcgcgta actctggcaa catcactgca ggatagcggt
    10741 caatttacct cctcaaacgc aacgcaaacc tagaacggct tcggcctatg attaacaaat
    10801 tactctatga attttggttt gcaggaaggc agcaagtgag tgaatccccg ggagcttaca
    10861 acagtaagtg acaggggtga acgaacgcaa ctgccgcacc tgtaagccaa aagacgacga
    10921 gtaaaatgcc aggttttact atggatacga aaaaactatt caagcacata ccctgggtga
    10981 ttctcggaat catcggtgca ttctgcctcg cggtagttgc attacgtcgg ggggagcacg
    11041 tcagcgccct gtggatcgtg gtcgcctctg tgtcggtgta tctggtggcg tatcgctact
    11101 acagtctgta catcgcccag aaggtgatga aactcgaccc cacgcgcgcg acgcctgcgg
    11161 ttattaacaa cgacggtctg aactacgttc cgaccaaccg ttacgtgttg tttggtcacc
    11221 acttcgccgc tatcgccggt gctggtccgc tggtcggtcc ggttctcgcc gcacagatgg
    11281 gctacctgcc tggcacgctg tggctgctgg cgggggtagt actggccggt gcggttcagg
    11341 actttatggt gctgtttatc tcctctcgcc gtaacggcgc atctctcggt gagatgatca
    11401 aagaagagat gggaccagta ccggggacta tcgcgctgtt tggctgtttc ttaatcatga
    11461 tcatcatcct cgccgtcctg gcgctgattg tggttaaagc cctggccgaa agtccgtggg
    11521 gtgtcttcac cgtttgctca accgtaccga ttgcgctgtt tatgggtatc tacatgcgct
    11581 ttatccgtcc ggggcgtgtg ggtgaagtct ctgtcattgg tatcgtgctg ctggttgcct
    11641 ctatctactt cggtggcgtg attgctcacg atccgtactg gggtccggca ctgaccttta
    11701 aagacaccac cattaccttc gcgctgattg gctatgcgtt tgtttccgca ctgctgccgg
    11761 tgtggctgat cctcgcaccg cgcgattatc tggcaacctt cctgaaaatc ggcgttatcg
    11821 tcggcctggc gctgggtatc gtggtgctga acccggaact gaaaatgcct gcgatgaccc
    11881 agtacattga cggtactggc ccgctatgga aaggcgctct gttcccgttc ctgttcatca
    11941 ccatcgcctg tggtgcggta tctggcttcc acgcgctgat ctcttccggt acgacgccga
    12001 aactgctggc taatgaaacc gacgcgcgtt tcattggcta cggcgcaatg ctgatggagt
    12061 ccttcgtggc gattatggcg ctggttgctg catccatcat cgaaccgggt ctttacttcg
    12121 cgatgaatac cccgcctgct ggccttggca tcaccatgcc taacctgcat gaaatgggtg
    12181 gcgagaacgc gccgatcatc atggcgcagc tgaaagacgt taccgcacac gcggcagcga
    12241 cagtcagctc ctggggcttc gtgatttcgc cagagcagat cctgcaaacc gcgaaagaca
    12301 ttggtgagcc ttctgtcctg aaccgtgcag gtggtgcgcc gacgctggcg gtaggtatcg
    12361 ctcacgtgtt ccacaaagtg ctgccgatgg ctgacatggg cttctggtat cacttcggta
    12421 ttctgttcga agccctgttc atcctgaccg cgctggatgc gggtactcgt tctggccgct
    12481 ttatgctgca agacctgctg ggtaacttca tcccgttcct gaaaaaaacc gactctctgg
    12541 ttgccggtat catcggtact gcgggctgtg tgggtctgtg gggctacctg ctgtatcagg
    12601 gcgtggtcga tccgctgggc ggcgttaaga gcctgtggcc gctgttcggt atttccaacc
    12661 agatgctggc agccgtagcg ctggtactgg gcaccgttgt gctgattaag atgaagcgca
    12721 cccaatacat ctgggtaact gttgttccgg ctgtatggct gcttatctgc accacctggg
    12781 cgctgggtct gaaactgttc agcaccaacc cgcagatgga aggcttcttc tacatggcaa
    12841 gccagtacaa agagaagatt gctaacggta ctgacctgac ggcgcagcag attgccaaca
    12901 tgaaccacat cgttgtgaac aactacacca acgcgggcct gagcattctg ttcctgattg
    12961 tggtgtacag catcatcttc tacggtttca aaacctggct tgcggtgcgt aacagcgaca
    13021 aacgtactga caaagaaaca ccgtacgttc caatcccgga aggcggcgtg aagatctctt
    13081 cgcaccacta accgtattta gccccgcttc ggcggggctt tgttcttatc agagtgaact
    13141 atgtttggta acttaggaca ggcaaaaaaa tatctcggcc aggcggcgaa gatgttgatt
    13201 ggcattccag actacgacaa ctacgtcgag catatgaaga ccaaccatcc cgacaagccg
    13261 tacatgagct atgaagaatt cttccgcgaa cgccagaacg cgcgctacgg cggcgatggt
    13321 aaaggcggta tgcgctgttg ttaatggaga gagcatgaac ccgattgcag ttaccctact
    13381 caccggtttt ttaggcgcgg gcaaaaccac cctgctgcgc catattctta atgaacaaca
    13441 cggctacaag attgccgtga ttgaaaacga attcggcgaa gtctccgttg atgatcaatt
    13501 gattggtgat cgcgcaacgc agatcaaaac gctgaccaac ggctgcatct gctgttcgcg
    13561 ctccaacgag ctggaggacg cgctactcga cctgctggat aatctcgaca agggcaatat
    13621 tcagttcgat cgtctggtca ttgaatgcac cggcatggcc gatcctggcc cgattattca
    13681 aacctttttc tcccatgaga ttttatgcca gcgctacctg ctggacggcg tgattgcgct
    13741 ggtggatgcg gtacatgccg atgagcagat gaaccagttc accatcgccc agtcccaggt
    13801 gggctacgcc gaccgtattc tgctgactaa aaccgacgtc gcaggcgaag cagaaaaact
    13861 gcgtgaacgc ctggcgcgca tcaacgcccg tgcaccggtc tacaccgtta cccacggcga
    13921 catcgacctg ggcctgctgt tcaacaccaa cggttttatg ctggaagaaa acgtcgtcag
    13981 caccaaaccg cgtttccact ttatcgcgga caaacaaaac gatatttcgt cgattgtggt
    14041 ggaactggat tacccggtag atatcagcga agtttcccgg gtgatggaaa acctgctgct
    14101 ggaatcggcg gataaactgc tgcgttacaa agggatgctg tggattgacg gcgaacctaa
    14161 ccgcctgctg ttccagggcg tccagcgcct ctacagcgcc gactgggacc gcccgtgggg
    14221 cgatgaaaaa ccgcatagca cgatggtgtt tatcgggatt cagttgccgg aggatgagat
    14281 tcgggcggcg tttgcgggac tcaaaaagta accagccctc caacatcaca atacctgaga
    14341 ctttatcacc ctcatattcc tccagaatat gagggcattc gggcttaatt atctctgaac
    14401 gtgcaacact tcctgcgact ccttatgcgc ctccacatcc tgcttcgtta accagagcga
    14461 cttacgacca aaattttcgt acattttcag ctgatcttca tagtgctgat caactgttcc
    14521 atcgggagca ataaacccac tctgaccggg tgcgaccaca tcccaggcaa gcaccggatg
    14581 atcgcttgtc gttggtgaga aaacaatcat atcgttttct gttccacggt tttgatactc
    14641 cgcctgatga cgcgtttctt ccgctgcggc ctgcggtaca ccaaagaaat tatttgcccg
    14701 gaacgttaag gccattgcag gtgttttcca gttactcaca ttattgccat agcgtttgga
    14761 aagagtctcc caggtatctt ccagcgcagc caacacaacc tcctgctgtg gtttcccagc
    14821 aaacagatca accgcctgtg ggattggtga tttgtctccc tgcaccgcct catacaaaat
    14881 ttttgctcca acacttatat tcagcgaacc agttgggccg tcctgggttg tttcgtagcc
    14941 actggcgctg taccacttat caaatggcat aggtacggca gccactacgg tacgcttcaa
    15001 catactggtc agccaaacgt tcaggatggc agagcctggc tgctgccagg ttttaccatc
    15061 atcattaagc aaattgatgc catcccaacg tgttaatgta tccaccaact gacggcgcgg
    15121 atcgctctgt gtcaaaccag atgtcgctgc ttgcagagta ggtaaaaaaa gcctcaggtt
    15181 aagatcctga cgactggttt ggcgaataac atcccatgcc tgatcagcag ttaagcgtgg
    15241 cttttgctca agcagtcggt cgatctccgt aacgcgatct gcccccccca caaaaaggca
    15301 aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc aatatatccc
    15361 gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt ccagtcccat
    15421 tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc tggataagca
    15481 ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt gatggtcagt
    15541 gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat ctgatgagtc
    15601 caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc gtaagccgtt
    15661 tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac agtaaaggtc
    15721 tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt cacccactta
    15781 ccattatgca agtagtagcc tggtttctct gcctacagcc gttcagcaaa aatatcgaca
    15841 tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc aaaaaccagc
    15901 ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg cagaccaata
    15961 ccataagtat acgcaggcgc ataccagcca aactacggac catttaccat gattgctttc
    16021 gcatcctggg ctttgctttt gccgatcacc cacatattgc tggtcgttgg ataccccgcc
    16081 agaccattgg caccaccctg tgcaaattgt gcagcaatag tttcccggtt cttccctgct
    16141 gttaacgcca gcagtgcgcc atccgcccct tttgctggtc ggtcaagcat tggtgcaggt
    16201 aaatcgtagc gtggcaacag agctgctgtt tgcgagtttt gctgattaaa tttaagtggg
    16261 tagctactct cttgtacggc aatagtggtt ggcgctgatg ggtttaccag ccatttcaac
    16321 tgattaaata ccgccatgcc ttgtgataca ccatatttat cttttaaagc cgttagcagt
    16381 gccagattat caatttcgct agtgctatca gagaagcggt ttgccatggt gcccacaaat
    16441 atcatcgcga catcaaacgg ttcccagcgc ttaggagtaa agccaaatgt attaaactgt
    16501 tttggtaaga gcgtctctgg attggtattt accttatcaa tccaggcatt cattccatca
    16561 gcgtagcctt gcagaatgga catatcctct ggggaaaggg cagcaatttg cgcccggata
    16621 gcatccggcc agtagttacg acggatatct ttatcaaatt tcacaaaatc tttgccaagc
    16681 acttccgcgg cagtcccttg agtactgcga cgtgccattt ccatctgaaa aaggcgatct
    16741 tgtgctacta catagccata gccataaaat aggtgccatg tatcattggc ataaatatgc
    16801 ggcatgccgt attcatcgcg aacaatcttt atctcacttg acgactgctc agccagtgca
    16861 ggtaagctcc aataatacat cagggaagca gtaacacagt tcacgatcat acgatttcta
    16921 tttttcattg tatcctctgg caggtgtata attagcgaat tgatactagc aacgaagctt
    16981 cggttatcta tttgcagaga attgtttcat tatgaactgt gagcgcgata actaattttt
    17041 gatacaaaaa tttcattaat gtgatctaaa acgaatcgct ttattcaccg ctgatgattt
    17101 tgttctattc ctttttgtga cagatccccc ggacgcatag atgatgaccc ccagagagac
    17161 agctcaggtc attatctgta tgtaaatcag aaaaatatgt tgtaaacgac ttttatggtt
    17221 ggattttgta gcaattttga atgcagacag gcagcatttg ccaacacgcc gcccatagtt
    17281 cactccacat cctccctgct taacccaata tccctcaacc gttcatcact catctgctgt
    17341 aacactcgtc gggtctgcat ttgccgccgc caacgcctca ccgcatgcca gagttgcaca
    17401 agtctgataa acggcgtttt ggctttgttt tcgtgaaatt ccatctgctg cctcctcatc
    17461 tttcagaggc ttcatcatcc gccttctggc agttcacaat acagatgcaa atattagttt
    17521 tatttaacat acagacaggt taaaacagca tctgcatccg gttttttagc ctcatctgta
    17581 ccggtttttc tttctgtatg ggccaaataa ggaatacagc atgacgcgtt atcaacatct
    17641 ggcgactcta cttgccgagc ggattgagca agggctgtat cgtcacgggg agaaattgcc
    17701 gtcggtgcgc agcttaagtc aggagcacgg cgtcagcatc agcaccgtgc aacaggcgta
    17761 tcagacgctg gaaacgatga agctcatcac tccgcagccg cgttcgggtt attttgtcgc
    17821 acaacgtaaa gcccagccgc ctgtaccgcc gatgacgcgt ccggtgcagc gcccggtgga
    17881 aattacccag tgggatcagg tgctggatat gctggtggcg catagcgaca gttccattgt
    17941 tccgttaagc aaaagcacgc cggatgtcga aacgcccagc ctgaaaccgc tctggcggga
    18001 gctaagccgg gtggtgcagc ataatctgca aaccgtgctc ggttatgact tgttagccgg
    18061 tcagcgggta ttgcgagagc agattgcccg cctgatgctc gacagcggct cggtggtcac
    18121 cgccgatgac atcatcatca ccagcggctg ccataactcg atgtcgctgg cgttaatggc
    18181 ggtgtgtaaa ccgggcgata ttgtcgcggt cgaatctccc tgttattacg gttcgatgca
    18241 gatgctgcga ggcatgggcg tgaaagtgat tgaaatccca accgatccag aaactggcat
    18301 cagcgttgaa gcactggaac tggcgctgga acagtggccg attaaaggca tcattctggt
    18361 gccaaactgt aataatccgc tgggatttat tatgccggac gcacgcaaac gggccgttct
    18421 ctctctcgct cagcgtcatg atattgtgat ttttgaagat gatgtctacg gcgaactggc
    18481 gacggagtat ccgcgcccgc ggaccattca ttcctgggat atcgacgggc gagtgctgtt
    18541 gtgcagctcg ttcagtaaaa gcattgcacc tggcctgcgc gtgggttggg tcgcaccggg
    18601 gcgctatcac gataaactgc tgcatatgaa atacgccatc agcagcttta atgtaccgtc
    18661 cacgcaaatg gcggcggcaa cgtttgtgct ggaaggtcac tatcatcgcc atatccggcg
    18721 gatgcggcag acttatcagc gcaatctggc gctttatacc tgctggatac gggaatattt
    18781 tccctgcgaa atctgtatta cgcgcccgaa aggcggattt ttactgtggg tagaattgcc
    18841 tgaacaggtc gatatggtct gcgtcgcgcg gcagctgtac cgcatgaaaa tccaggtggc
    18901 ggcaggctcg attttctcag cgtccggcaa ataccgtaat tgtctgcgca tcaactgcgc
    18961 tttaccgctc agcgaaacct atcgcgaagc actaaagcaa attggcgagg ccgtgtatca
    19021 ggcaatggaa tgaattcgaa atagtggcga agcacctgat ttactttcac gaatcgcctg
    19081 taactcgttt tatctttata gcgacatcgc cacgttccgg catagcttag gcaaagtgtg
    19141 ctttgttcat gccggatgcg gcgtgaacgc cttatccggc ctacaaaacc agcaaattca
    19201 atagattgca gaggtaatgt aggtctgata agcgtagcac atcaggcagc tttgcgtttg
    19261 tcatcagcct ctgattagta ttcgatcaat ggatcgcgct tgctttcagg caagggctga
    19321 tctaatgcct gcatttgctc tgccgaaagt ccggtatatc gttgtatcca ttcaggatcg
    19381 gccccattct gcaacaacaa tcggaggagg ctccgttccc cttctacttt cccttctttc
    19441 ctccccctct cccttcccaa cagccatcca tcattatgaa ttcgctctgc aattgtcatt
    19501 attcgctccc tgtggtgtgg cattcgcctg gtaagttcgc tgataaacgc cttaaaacgc
    19561 gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag ttatctggct gtcattagca
    19621 cactctgtaa ccagtaatac taccagttgg tcgataagcc ccatcagatc gcgctggcga
    19681 atatgctttt ggatcaactc caacagggcg actctgcgat gctgcacaat ctcgtcgtct
    19741 ggcacgacag tgacatccac cagcgggaac gctgcgctat aaagtttccg tgcggtagtc
    19801 gggtcggcaa attcgtccag ccagcacagg gaccagggat aaggactacg tctaccgtga
    19861 taaaatagca tcgggatgac cagcggtagc ggccgacgtt tatcatgctc gatatggcgc
    19921 tgcatcaccg ccatggaata gcgcatcagg cgaaaggcca tatgaatgtc ctcgcggctc
    19981 tgatgttcaa tcaccacata gatataacca tcgccttcgc gggtctttac cgaccacaga
    20041 atatcggaat gtagcgcccg caatttttca tcgacaaagc tggcggattc cagttttaag
    20101 ctgtcgagat cgcacagttc acgtaaatct ttaggtaagt ggatctccat aaaatcccgc
    20161 gcggtgtcag ggtgcgtgag aaaggattta aataatgcgt catgcggcgt gctggtcgag
    20221 aagtttgtca tggcgatccg tcacctgtag ataagatggc ccgactttag cggctaacaa
    20281 aaccattctc acccggcaat ttcccgttcc ccgaggcgct ttccaggcta tttttattct
    20341 tctgcaaacg atacattttt tcggaaacag gctcgcgttt tacatttcct gtacccggca
    20401 gatattcccc tttccaccgc gctcttttga ttaaccgcaa attttaatta cacttaaggt
    20461 gtatattttc tatgcaaccc atcaattaaa gaggtgtaat gtgctgatga ctatttgtaa
    20521 tcgttataca ttctgacctg gagtcagaag gtatttctct gtctgtgtgt tcacaggcag
    20581 tgtggttgat tacatgaaat tcagtacatt tgtccttctc gcctgccttt cgtcattacc
    20641 gacggtattg aatttcgttt tccccgttgg ggttctccgg acaaggagtt gtttgttatg
    20701 ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc cgatggcgtt gattttgtat
    20761 gactttgccg cgtacctgtc gacggatctg atccagccgg ggatcattaa tgtggtccgt
    20821 gattttaatg ccgatgtcag tctggccccg gcagccgtca gtctctatct cgcgggcggt
    20881 atggcgttac agtggctgct ggggccgctt tccgacagga ttggccgcag gccggtgctg
    20941 attaccgggg cgctaatatt tacccttgcc tgcgctgcga caatgttcac cacgtctatg
    21001 acacagtttc ttatcgcgcg tgcaattcag ggcaccagta tctgttttat tgccaccgtt
    21061 ggttatgtca cggtgcagga ggcgttcgga cagacaaaag ggattaagtt gatggcgatt
    21121 atcacctcca tcgtactgat tgcgccgatt attggcccgc tttccggcgc agctctgatg
    21181 cactttgtac actggaaagt cctttttgcc atcattgcgg ttatgggttt tatctcattt
    21241 gttggcttac tgctggcgat gccagagacg gtgaagcgcg gcgcggttcc atttagcgcc
    21301 aaaagcgtct tgcgcgattt tcgtaatgtc ttttgcaatc ggctgttcct ctttggcgca
    21361 gcaaccatct ctttaagcta tatcccaatg atgagttggg tggctgtctc gccggtgatc
    21421 ctgatcgacg cgggcggctt aacaacttcg cagttcgcct ggacacaggt tccggtgttc
    21481 ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg ttaaagatcc gaccgaaccg
    21541 cgctttatct ggcgtgctgt acccattcaa ctggtcggcc tcgcgctgtt gattatcggc
    21601 aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg gcaccagttt gtatgctttc
    21661 gggattggtt tgatcttccc gaccttattc cgctttacgc ttttttccaa taacttaccg
    21721 aaagggagcg tctccgcatc gctgaatatg gtgatcctga tggtgatgtc tgtctcggtc
    21781 gaaatcggcc gctggctgtg gtttaacggc ggtcgcttgc cgtttcatct gttagccgtc
    21841 gtggcgggcg ttatcgtcgt tttcaccctg gcaggattgc ttaatcgcgt gcgccaacat
    21901 caggctgccg agctggcgga ggagcggtga tttttgcgcg atcctgccgt caggctctat
    21961 tcttaacgtt atgaataaac tcattgaact cagacgcgcc aaaatgttgg cgctctcttt
    22021 actgcttatc gccgctgcta cctttgtcgt tacgctgttt ttgccgccca atttttgggt
    22081 gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc gcgctggcgg actggtttgc
    22141 ggtggtggcg ctgtttcgcc gcgtgccgat tccgatcatt tctcgtcata cggcgattat
    22201 cccgcgtaat aaagaccgga ttggcgaaaa tctcggccag ttcgtgcagg aaaaatttct
    22261 cgatacccag tcgctggtgg cattgattcg acgtcacgaa ccggcgttgt tgattggcaa
    22321 ctggtttagt cagccggaga acgcccgccg cgttggtcag catctgttgc agatcatgag
    22381 cggttttctt gaactgaccg atgatgcacg tattcagcgc ctgcttaagc gcgcggtcca
    22441 tcgggcgatt gataaggtcg atctttccgg aaccagtgcg ttgatgctgg agagtatgac
    22501 caaaaacgat cgtcatcagg tgctgctcga tacgctgatc gcacagttga tcgcccttct
    22561 ccagcgcgat aaatcgcgca agtttattgc ccagcagatt gttcgctggc tggagagtga
    22621 gcatccactg aaagccaaaa ttctccccac cgaatggttg ggcgaacata gcgcggagtt
    22681 ggtttctgac gcggtgaatt ctttgcttga tgatatcagc cgtgatcgtg cgcatcagat
    22741 ccgtcatgcg tttgatcgcg ccacttttgc cctgatcgac aagttgaaaa acgatacgga
    22801 aatggcagcg cgagccgatg ccgtaaaaag ttatctgaaa gaagatgaag cttttaaccg
    22861 ctatctcagt gaattgtggg gggatttacg ggaatggctg aaagcggata tcaacagtga
    22921 agattctcgt gtgaaagaac gtatcgcgcg ggcgggtcaa tggtttggcg aaacgttaat
    22981 tgccgatgat gccttgcggg catcgttaaa tggtcacctg gaacaagccg cgcaccgcgt
    23041 cgcgcctgag ttttccgcat tcctgacgcg ccacatcagc gatacagtaa aaagctggga
    23101 tgctcgggat atgtcgcggc aaatcgagtt aaatatcggc aaagatctgc agtttatccg
    23161 tgtcaacggt acgctggttg gcggttgtat tgggctaatt ttatatttgt tgtcgcagct
    23221 cccggccttg ttccccctca gcaattttta gaaatccatg aaaaactaag tggctaaccg
    23281 cgagagagat caaataaacc acactaaaag aatggtaata atgtggcctc ttttcattat
    23341 ctcccgtggt acggggaagg aaaatcatgt cacttatcac cgatctaccc gccattttcg
    23401 atcagttctc tgaagctcgc cagaaaggct ttctcaccgt catggatctc aaggagcgcg
    23461 gcattccgct ggttggcact tactgcacct ttatgccgca agagatcccg atggcagccg
    23521 gtgcggttgt ggtttcgctc tgttctacct ctgatgaaac cattgaagaa gcggagaaag
    23581 atctgccgcg caacctctgc ccgctgatta aaagcagcta cggctttggc aaaaccgata
    23641 aatgccccta cttctacttt tcggatctgg tggtcggtga aaccacctgc gacggcaaaa
    23701 agaaaatgta tgaatacatg gcggagttta agcccgttca tgtgatgcag ttgccgaaca
    23761 gcgttaagga cgatgcctcg cgtgcgttat ggaaagccga gatgctgcgc ttgcaaaaaa
    23821 cggtagaaga acgttttggg cacgagatta gcgaagatgc tctgcgcgat gccattgcgc
    23881 tgaaaaaccg cgaacgtcgc gcactggcta atttttatca tcttgggcag ttaaatcctc
    23941 cggcgcttag cggcagcgac attctgaaag tggtctacgg cgcaaccttc cggttcgata
    24001 aagaggcgtt gatcaatgaa ctggacgcga tgacagcccg cgttcgtcag cagtgggaag
    24061 aaggccagcg gctggccctg cgtccacgca ttttaatcac cggctgcccg attggcggcg
    24121 cagcagaaaa agtggtgcgc gcgattgaag agaatggcgg ctgggttgtc ggttatgaaa
    24181 actgcaccgg ggcgaaagcg accgagcaat gcgtggcaga aacgggcgat gtctacgacg
    24241 cactggcgga taaatatctg gcgattggct gctcctgtgt ttcgccgaac gatcagcgcc
    24301 tgcaaatgct cagccagatg gtggaagaat atcaggtcga tggcgtagtt gatgtgattt
    24361 tgcaggcgtg ccatacctac gcggtggaat cgctggcgat taaacgtcat gtgcgtcagc
    24421 agcacaacat tccttatatc gctattgaaa cagactactc cacctcagat gtcgggcagc
    24481 tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg agtggcagtg acatattcga
    24541 ttggcattga ttctggttct accgccacca aagggatctt actggcagac ggcgtgatta
    24601 cgcgccgttt cctcgttcca acaccctttc gtccggcaac agcaattact gaagcctggg
    24661 aaactctgcg cgaagggtta gagacaacgc cgtttctgac gctcaccggc tatggacggc
    24721 aactggtgga ttttgccgat aaacaagtga cggaaatctc ctgtcatggg ctgggcgcac
    24781 ggtttcttgc gccagcaacg cgcgcggtaa tcgacatcgg tggtcaggac agcaaagtga
    24841 ttcagcttga tgatgacggt aacctgtgcg atttcctgat gaatgacaaa tgcgcggcgg
    24901 gcaccgggcg tttcctggag gtgatctcgc gcacgcttgg caccagcgtc gagcaactcg
    24961 acagcattac cgaaaatgtc acgccgcacg ccatcacgag tatgtgcaca gtgtttgccg
    25021 aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc agaagcgatt ctcgccggag
    25081 tgattaacgc gatggcgcgg aggagtgcca atttcattgc tcgtctctcc tgtgaagcgc
    25141 cgattctgtt tactggtggc gttagtcatt gccagacgtt tgcccggatg ctggaatctc
    25201 atctgggaat gccggtaaat acccatcctg atgcgcaatt tgctggcgca attggcgcgg
    25261 cggtaattgg tcaacgagtg aggacacgcc gatgaaagag tttttatttt tatttcactc
    25321 cacagtcggc gtcatacaaa cccgcaaagc gttgcaggca gcgggcatga cttttcgcgt
    25381 cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta tgcatttggc tgacctgtcc
    25441 ccccggcgag gaaatacaat gggtgatccc tgggctgact gagtcaattt attgccagca
    25501 ggatggtgtc tggcgctgca ttgcacatta ccgggtttct ccgcgttaag gattcattaa
    25561 ggtcgctacg tatacttctc gatatcttat tgatttgcct taaagggatt accgtgacaa
    25621 aaagtatttc gttaagtaaa cgcatctccg ttattgtgat tctttttgcc attgtggcag
    25681 tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa tcatgcagca tcatttcaga
    25741 attaccacgc caccattgac ggcaaagaaa ttgcaggcat aacgaataat atctcttcgc
    25801 tgacctggtc ggcacaaagc aatactcttt ttagcactat caacaaacct gccgccattg
    25861 ttgaaatgac caccaatggt gattttatcc gcacaattcc tctggatttt gttaaggatc
    25921 tcgagacgat cgaatacatt ggcgataatc agtttgtcat tagcgatgag cgcgattacg
    25981 ccatttatgt gatttcactg accccaaact cggaagtaaa aatcctcaaa aaaataaaaa
    26041 tcccgctgca agactcccca accaattgcg gttttgaagg gttggcttat tctcgccagg
    26101 atcacacttt ctggtttttt aaagagaaaa atccgattga ggtctacaaa gttaacgggc
    26161 tgttaagtag caatgaatta cacatcagca aagacgaggc actacaacgg caatttactt
    26221 tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa tacgcttctg gtgctgtctc
    26281 atgaatctcg cgcactccag gaagtaaccc tggttggcga ggttatcggt gaaatatcac
    26341 taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca ggccgaaggg attgcgatgg
    26401 atgcctctgg taatatttat atcgttagcg aaccaaatcg tttttatcgc tttaccccta
    26461 aatcatcgca ttaacaggct ggcatatttc acctttttgt tattaacttg ttaacaacaa
    26521 aaatgacata caaggttaaa catgccttcg tcctcgcatc ccgtagaacg cttttctttc
    26581 agcaccgcgc ttttcgggat gctggttctg accctaggta tgggattagg tcgcttcctt
    26641 tatacgccta tgttgcccgt catgatggcg gaaggggcgt tttcatttag ccagctctcg
    26701 tggattgcca gcggcaacta tgcggggtat ctggctggca gtctgctatt ttcgtttggc
    26761 gcatttcacc agccgtcgcg cctacgccca tttctgttgg tttctgccct ggcgagcggg
    26821 ttgttgatcc tcgcaatggc atggttgccg ccatttattc tggtgttatt gattcgcttc
    26881 ctggcgggtg tcgccagcgc cggtatgctg atttttggtt cgacgctgat tatgcagcac
    26941 acgcgccatc cttttgtgct ggcagctttg ttttctggcg ttggcattgg cattgcactg
    27001 ggcaacgaat atgttctggc aggcctgcat tttgccttat cctcgcaaac gttatggcaa
    27061 ggcgccgggg cgctttctgg catgatgctg attgcactta cgcttttaat gccctcgaaa
    27121 aaacacgcca tcgcaccaat gccattggca aaaacggagc aacagataat gagctggtgg
    27181 ttactggcta ttctgtacgg cctggcggga tttggttata tcatcgtcgc gacttacctg
    27241 ccgctcatgg cgaaagatgc aggctcacca ttgttaaccg cccatctctg gacgttagtc
    27301 ggcttatcaa ttgtgccagg ctgctttagc tggctatggg cagcaaaacg ttggggggct
    27361 ttgccttgcc tgacggcgaa tttgctggtg caggctatca gtgtgctgct tactctcgcc
    27421 agcgactctc ctctcttgct tatcattagt agtatcggtt ttggcggtac ctttatgggc
    27481 acgacatctc tggtgatgac tatcgcccgc caactgagcg ttccgggaaa tctcaatctt
    27541 ttaggctttg tgacgctcat ttacggcatt ggacaaatcc tcggcccggc gctgaccagt
    27601 atgctcggca atggaacatc ggcgcttgcc ggcgccactc tctgcggcgc ggcggcgttg
    27661 tttattgctg cgcttatcag cacagtacaa ttatttaagc tgcaagtggt cacttcatga
    27721 ggagtgtttc ccttaccact caagaaattc gaccgcaacc gttgatgtca gccagactcc
    27781 gttttccgct tgccagaaag ggatgcctcg tttcgccatt tcctgtgctt tgacggttaa
    27841 gataaccggc gatccgtgtc ttgctcccac tttgcgtgcc gttgcttcat cagcagagag
    27901 atgcacgtaa tgacgctccc ctgcaatcag cccctgtttt tttatctcgt caaggaaacg
    27961 gcttgccgta ccgtggtaaa gaaactgtgg cggtgttttt tcggcaaagg aaatggcgac
    28021 ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta ccatcactgg aataactaaa
    28081 acgcttttta tcactggtag cgaccacggt atccagcaat gcacgggtta accgcttgcc
    28141 cgctttttgg gcacagagta taagcttatc gatatccgcc catccttcac ggtcaagtac
    28201 gataccaatc gcctccggtt tgtgtcggag gacaaaactt aaaaacttgc tcgtatccgc
    28261 caactctttt tcgttgtact ttgccatctc gttttcgtcc ctggattttc tgtttttatt
    28321 tactgatgtc attgtcactg ggtttatgaa ttttctgcaa ctcaatcctg cgcagagcct
    28381 tttccacaca gcataatttt tccgctatgc aaaaaacgca tagccagaaa catacttgca
    28441 ttttccccat acccacatgg ctgataaggt ttttccgctc caggaatttt cctggctcaa
    28501 gcaacacaca aacacaaaaa aacaatgggg attgttatga cacaacaagg ggatgctgta
    28561 gccggggagc tggcgacaga aaaagtgggc attaaagggt atctggcttt ttttctcacc
    28621 ataatctttt tttccggtgt ttttccggaa cagacagctg gtggcgcgtt tttgattttt
    28681 ccgttctgaa cggatcgttt gggcaactgc ctggtgcgaa cggagccacc acctcatttc
    28741 gcggggcggg tggcgcgggg gctaaagatg gctttctctt tgcgctggag ctggcaccgt
    28801 cagtcattct ctctctgggg attatttcaa ttaccgatgg tcttggtgga ttacgcgccg
    28861 cgcagcagtt aatgacgcct gtgctaaaac cgttgttggg cattccaggg atctgctcgc
    28921 tggcgctgat cgctaacctg caaaacactg atgccgccgc cggtatgacc aaagagctgg
    28981 cgcaggaagg ggaaattacc gaacgcgata aagtgatttt cgccgcatat caaaccagcg
    29041 gtagcgccat catcaccaac tacttctctt ccggcgtcgc ggtgtttgcc tttctgggca
    29101 cgtccgtaat cgtaccttta gccgtcatcc tggtgtttaa atttgtcgga gccaacattt
    29161 tgcgcgtctg gcttaacttc gaagaacgtc gcaacccaac gcaaggagcg caagcatgac
    29221 aactcaggtc cgcaaaaatg tcatggatat gtttatcgat ggtgcgcgcc gtggctttac
    29281 tatcgccacg accaacctgc tgccaaacgt ggtgatggcg tttgtcatca ttcaggcgct
    29341 gaaaattacc ggcctgctcg actgggtggg gcatatctgt gaacccgtaa tggcgctgtg
    29401 gggattaccc ggtgaagcgg caaccgtcct tttagccgca ttaatgagta tgggcggcgc
    29461 agtcggtgtt gccgccagtc tggcgaccgc cggcgcatta accggtcacg atgtcaccgt
    29521 tttactgccc gcgatgtatt taatgggcaa cccggtgcaa aatgtcggtc gctgcctggg
    29581 gacagccgaa gtgaatgcca aatattaccc gcatatcatt acggtctgcg tgattaacgc
    29641 attgctgtcg atctgggtta tgcagcttat tgtttaacaa ggagttacca tgattgatta
    29701 taccgcagcc ggttttaccc tgctgcaggg agcgcatttg tatgcgccgg aagatcgggg
    29761 aatttgcgat gtcctcgtcg ctaacggcaa aattatcgcc gttgccagca atatcccttc
    29821 tgacattgta ccggactgca cggttgtcga tctcagcggg cagatcctct gtcctggttt
    29881 tattgatcaa cacgtccatt tgattggtgg tggcggcgaa gcaggtccca cgacgcgcac
    29941 gccagaagtg gcgctaagtc gcctgacgga agcgggcgtc acgtcagtgg ttggtctgct
    30001 gggcaccgac tctatctctc gccacccgga atccctgctc gccaagaccc gtgcgctcaa
    30061 tgaagaaggc atcagcgcct ggatgctgac cggcgcttat catgtccctt cccgcaccat
    30121 tacaggttcc gtggaaaaag acgtggcgat tatcgatcgt gtgattggcg tgaaatgcgc
    30181 catctctgat caccgttctg ccgcaccgga cgtttatcac ctggctaata tggcggcgga
    30241 atcccgcgtt ggcggtttgc tcggcggtaa acctggcgtc accgtgttcc acatgggcga
    30301 cagcaaaaag gcgttacagc ctgtctatga cctgctggaa aactgcgatg tgccgatcag
    30361 caagttgctg ccgacccacg ttaaccgcaa cgttccgttg tttgagcagg cgctggagtt
    30421 cgcgcgcaaa ggcggcacta tcgatatcac cagcagcatt gacgaaccgg tcgcccctgc
    30481 cgaaggtatt gcccgcgccg ttcaggcggg tattccgctg gcacgcgtca ccctcagctc
    30541 cgacggcaac ggtagccagc cgttcttcga tgacgaaggg aatttaaccc atatcggtgt
    30601 tgccggtttt gaaacgttgc tggaaaccgt gcaggtgctg gtcaaagact atgatttcag
    30661 tatcagcgat gccctgcgcc cgctcaccag tagcgtagcc ggtttcctta acctgaccgg
    30721 gaaaggcgaa attctgccag gcaatgatgc tgacttgctg gtcatgacgc cagaactgcg
    30781 cattgagcag gtatacgctc gcggcaaact gatggtcaaa gacggcaaag cctgcgtgaa
    30841 aggaacgttt gaaacggctt aaacggttgt cagccgcccg aatggacgta gaatgcccgc
    30901 aagggcggct gacagagtaa aacgtaatgg atgactgtgg tgcgattttg cataatattg
    30961 aaaccaaatg gctttatgat tttctgaccc tggaaaaatg ccgcaatttt tccctggcgg
    31021 cagtcagtcg caacgtctcg caaccggcat tcagccgccg catccgtgcg ctggaacagg
    31081 cgattggtgt tgaattgttt aaccgccagg taacgccgct gcaactctca gaacaaggga
    31141 aaatcttcca ttcgcagatc cgccatctgt tgcaacagtt agagagcaat ctggcagagc
    31201 tgcgtggcgg cagcgattac gcgcaacgaa aaatcaagat agccgctgca cactctcttt
    31261 ccctcgggct gttaccgtcc attatcagcc agatgccgcc gctctttacc tgggcaattg
    31321 aagctattga tgtcgatgaa gcggtcgata aactgcgtga agggcaaagt gactgtattt
    31381 tttcctttca cgacgaagat ttgctggaag cgccgtttga ccacattcgc ttatttgaat
    31441 ctcaattgtt ccctgtctgc gccagtgacg aacacggaga agcacttttt aacctcgcgc
    31501 agccacactt tccgttactg aattacagcc gcaactccta catggggcga ttgattaatc
    31561 gcaccctgac gcgccacagt gagttaagtt tcagcacctt ttttgtctct tcgatgagcg
    31621 agcttttaaa gcaggttgcc ctcgacggct gtgggattgc ctggctgccg gagtacgcca
    31681 tccaacaaga aattcgcagc gggaagctcg ttgtgctgaa tcgggacgaa ctggtgatcc
    31741 cgatccaggc ctacgcatac cggatgaata ctcgcatgaa tcccgttgcc gaacgcttct
    31801 ggcgtgaact gcgcgagctg gagattgtgc ttagctgaca ttctccagca tcgcactaca
    31861 ccaaccgcca ttatcccaga ccagatcaaa cgttagcggt tcaccgtcgt ggtacagcat
    31921 tatctcaagg ctgatcgcca gagtgtggca gtacgcttct ttaaaattaa cctgcaacgc
    31981 cgcaatccgt ggctcatgaa ataacagtgc agaacgcagc tgacggcaat accagctctg
    32041 gagatgtttg tcgctctggt tatgcacttg ccactcgggc atgcctgaaa caagtaatga
    32101 cggtaatgcc gaatgaggag aattaaaaag agtgaggata tcgctgcata ccgatttacg
    32161 cagcaatgac gttttattgc ctgaaatttc aggtaaaaca gcctgaagtg attggcgcat
    32221 cattttgcgc actcctgacg tttagcaaca tatgcggctt gccctgaaca ccgggcacca
    32281 ctgtattgat gctcattttc catagccatt cccttttgtt cattacatcc atgtcagtct
    32341 gagacaggcc tgaatcctgt cagcaaagta taaaacctta cttgcctatg aatatctact
    32401 gacttttcag gaaaaaagtc atgatttgat aaaaatcatc aaactcaaaa aaaaaccgct
    32461 agtatattct gaatattccg aatagttacg tagatgaaaa tacttgtcac cgccgctact
    32521 taattccggt ggaaa
//
LOCUS       DAZKIV010000036        32287 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZKIV010000036 DAZKIV010000000
VERSION     DAZKIV010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.036"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHOT6_001703"
     CDS             complement(39..1403)
                     /gene="rhlE"
                     /locus_tag="ACHOT6_001703"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFP9946936.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NICSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            1632..2303
                     /gene="cecR"
                     /locus_tag="ACHOT6_001704"
     CDS             1632..2303
                     /gene="cecR"
                     /locus_tag="ACHOT6_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFP9946937.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHOT6_001705"
     CDS             2306..3301
                     /gene="hlyD"
                     /locus_tag="ACHOT6_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFP9946938.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTELVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            3294..5030
                     /gene="ybhF"
                     /locus_tag="ACHOT6_001706"
     CDS             3294..5030
                     /gene="ybhF"
                     /locus_tag="ACHOT6_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFP9946939.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            5023..6156
                     /gene="ybhS"
                     /locus_tag="ACHOT6_001707"
     CDS             5023..6156
                     /gene="ybhS"
                     /locus_tag="ACHOT6_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946940.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRTVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            6167..7273
                     /gene="ybhR"
                     /locus_tag="ACHOT6_001708"
     CDS             6167..7273
                     /gene="ybhR"
                     /locus_tag="ACHOT6_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9946941.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(7235..7645)
                     /gene="ybhQ"
                     /locus_tag="ACHOT6_001709"
     CDS             complement(7235..7645)
                     /gene="ybhQ"
                     /locus_tag="ACHOT6_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFP9946942.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGPAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            7778..8539
                     /gene="ybhP"
                     /locus_tag="ACHOT6_001710"
     CDS             7778..8539
                     /gene="ybhP"
                     /locus_tag="ACHOT6_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9946943.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            8536..9777
                     /gene="clsB"
                     /locus_tag="ACHOT6_001711"
     CDS             8536..9777
                     /gene="clsB"
                     /locus_tag="ACHOT6_001711"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFP9946944.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            9777..10733
                     /gene="ybhN"
                     /locus_tag="ACHOT6_001712"
     CDS             9777..10733
                     /gene="ybhN"
                     /locus_tag="ACHOT6_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFP9946945.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGT
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(10769..11482)
                     /gene="ybhM"
                     /locus_tag="ACHOT6_001713"
                     /pseudo
     CDS             complement(10769..11482)
                     /gene="ybhM"
                     /locus_tag="ACHOT6_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(11511..12302)
                     /locus_tag="ACHOT6_001714"
     CDS             complement(11511..12302)
                     /locus_tag="ACHOT6_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9946946.1"
                     /translation="MPDYVHYVISGTDNAFEQTIHCYSITAKKCFAQQNAASHLVVNS
                     SWSMGYGRLCLDISAFALGYVTKVSGAFVFALAALIIGGLWLNNALKRLNVSGACLVY
                     LLIILLVGFLFASLYTFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCG
                     LSIAFVVNLILTSSFSVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCI
                     VPGAITLYFSILSVLFRITVTVLEFINGFSVVVRIDEFYCVNWNR"
     gene            complement(12401..13105)
                     /gene="ybhL"
                     /locus_tag="ACHOT6_001715"
     CDS             complement(12401..13105)
                     /gene="ybhL"
                     /locus_tag="ACHOT6_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFP9946947.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYSWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(13242..13694)
                     /gene="moaE"
                     /locus_tag="ACHOT6_001716"
     CDS             complement(13242..13694)
                     /gene="moaE"
                     /locus_tag="ACHOT6_001716"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFP9946948.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(13696..13941)
                     /gene="moaD"
                     /locus_tag="ACHOT6_001717"
     CDS             complement(13696..13941)
                     /gene="moaD"
                     /locus_tag="ACHOT6_001717"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFP9946949.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(13934..14419)
                     /gene="moaC"
                     /locus_tag="ACHOT6_001718"
     CDS             complement(13934..14419)
                     /gene="moaC"
                     /locus_tag="ACHOT6_001718"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFP9946950.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNWVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(14422..14934)
                     /gene="moaB"
                     /locus_tag="ACHOT6_001719"
     CDS             complement(14422..14934)
                     /gene="moaB"
                     /locus_tag="ACHOT6_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFP9946951.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(14956..15945)
                     /gene="moaA"
                     /locus_tag="ACHOT6_001720"
     CDS             complement(14956..15945)
                     /gene="moaA"
                     /locus_tag="ACHOT6_001720"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFP9946952.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(15933..16077)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            16342..17250
                     /gene="yvcK"
                     /locus_tag="ACHOT6_001721"
     CDS             16342..17250
                     /gene="yvcK"
                     /locus_tag="ACHOT6_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFP9946953.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSIASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(17442..19463)
                     /gene="uvrB"
                     /locus_tag="ACHOT6_001722"
     CDS             complement(17442..19463)
                     /gene="uvrB"
                     /locus_tag="ACHOT6_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFP9946954.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(20043..20720)
                     /gene="bioD"
                     /locus_tag="ACHOT6_001723"
     CDS             complement(20043..20720)
                     /gene="bioD"
                     /locus_tag="ACHOT6_001723"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFP9946955.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(20713..21468)
                     /gene="bioC"
                     /locus_tag="ACHOT6_001724"
     CDS             complement(20713..21468)
                     /gene="bioC"
                     /locus_tag="ACHOT6_001724"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFP9946956.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHALVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     SRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(21455..22609)
                     /gene="bioF"
                     /locus_tag="ACHOT6_001725"
     CDS             complement(21455..22609)
                     /gene="bioF"
                     /locus_tag="ACHOT6_001725"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFP9946957.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDIAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(22606..23646)
                     /gene="bioB"
                     /locus_tag="ACHOT6_001726"
     CDS             complement(22606..23646)
                     /gene="bioB"
                     /locus_tag="ACHOT6_001726"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFP9946958.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSKRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            23733..25022
                     /gene="bioA"
                     /locus_tag="ACHOT6_001727"
     CDS             23733..25022
                     /gene="bioA"
                     /locus_tag="ACHOT6_001727"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFP9946959.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQK
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            25081..25557
                     /gene="ybhB"
                     /locus_tag="ACHOT6_001728"
     CDS             25081..25557
                     /gene="ybhB"
                     /locus_tag="ACHOT6_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFP9946960.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            25709..26992
                     /gene="ybhC"
                     /locus_tag="ACHOT6_001729"
     CDS             25709..26992
                     /gene="ybhC"
                     /locus_tag="ACHOT6_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFP9946961.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVN
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(27226..29487)
                     /gene="ybhJ"
                     /locus_tag="ACHOT6_001730"
     CDS             complement(27226..29487)
                     /gene="ybhJ"
                     /locus_tag="ACHOT6_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFP9946962.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(29670..31103)
                     /gene="ybhI"
                     /locus_tag="ACHOT6_001731"
     CDS             complement(29670..31103)
                     /gene="ybhI"
                     /locus_tag="ACHOT6_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFP9946963.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLMVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            complement(31179..32231)
                     /gene="ybhH"
                     /locus_tag="ACHOT6_001732"
     CDS             complement(31179..32231)
                     /gene="ybhH"
                     /locus_tag="ACHOT6_001732"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFP9946964.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
BASE COUNT         7715 a         8621 c         8293 g         7658 t
ORIGIN      
        1 tcatcagcct gatgccgggc atagcccggc attaaagatt actgcgcagc agcaggttta
       61 cgcggacggc ggcgacgttg ttgttcgcct gccggtttgg catcgccgag gcggcgagac
      121 ggtttttctg caggtttcgc gcttgcagat tttgcgccac cttccccacg gcgtggttgc
      181 tgttgaccgc gaccaccacc ttgcccacga ccgccgccgc cacgttgctg gcgaccgttc
      241 tggatcggtt cggctttgat tgacgggtcc ggctcatagc ccggaatcgc aatgcgcggg
      301 atctcttttt tcagcagttt ttcgatatca cgcagcagtt tgtgttcatc aacacacacc
      361 agcgacaacg cttcaccggt agcagccgca cgaccggtac gcccgatacg gtggacataa
      421 tcttccggta cgtttggcag ttcatagttg acgacgtgcg gcaactcttc gatatccaga
      481 ccgcgcgcag cgatatcggt cgccaccagt acgcgaatgt cacccgattt aaaatcagcc
      541 agcgcacggg tacgcgcacc ttgcgattta ttcccgtgga tcgccgcact acggatgcca
      601 tctttattga gctgctcagc cagatggtta gcgccgtgtt tggtacgggt aaacaccagc
      661 acctgctgcc agttcccttt accaatcatg tgcgacagca attcgcgttt gcgtttctta
      721 tcgacaaagt gaacgtgctg ggtcacctga tcagacgcgg tattgcggcg tgccacttcg
      781 atttccagcg ggttgtgcaa cagtttttcc gccagggctt taatatcgtc agagaaggtc
      841 gcagagaaca gcaggttctg gcgtttcgct ggcagttttg ttaacacgcg acgaatatcg
      901 tggataaagc ccatgtcgag catgcggtca gcttcatcga ggacgaggat ttcaacctga
      961 tccagcttca ctgcattttg atgttccagg tccagcaaac gtcccggtgt tgccaccaga
     1021 acatcaacgc cgccgcgcag cttcatcatt tgcgggttaa tgctgacacc gccaaacacc
     1081 accagcgaac aaatgttcaa atatttgctg taatcacgga cgttttcgcc aatctgcgcc
     1141 gccagttcac gggtcggggt aagaatgagc gcacgtaccg gacgacgccc tttggcgtgc
     1201 ggctggcgag tgatcaggtg ttgcaacagc ggcagcgtaa agcccgctgt tttaccggta
     1261 ccggtctggg cgctagccat caggtcgcgg ccttccagca ccgcagggat cgcctgctgc
     1321 tgaatagggg tgggttcacg gtaaccctgc tcggcaacgg cgcgcaggat atcagggctt
     1381 aaacccaaag aatcgaaaga cataactact ccgaaccgcc ccgaccgtta ccggtgtagt
     1441 tttcagggag atactgaaaa ggaaaatgac aaaaaccaca atgtcatgag ggagcggagt
     1501 gtagcagttt ttgtgacgca gcgcataaat tatccctgtg actggcgtag ctaaaattct
     1561 tagccatact ggacaactcc catatggagt cataatctta atcaatcatt tgattaagaa
     1621 tggactgtgc gatgaataat cctgccatga caatcaaggg tgaacaggcg aaaaaacagc
     1681 tgattgctgc cgcactggcg cagtttggtg aatatggaat gaacgccacc acccgcgaga
     1741 tagccgccca ggccgggcag aatatcgctg ccatcaccta ctacttcggt tcgaaagaag
     1801 atttgtacct cgcctgcgcc cagtggattg ccgattttat tggcgagcag ttccgtccgc
     1861 atgccgagga agccgaacgc ttgttcgccc aaccacagcc tgatcgggct gccatccgtg
     1921 aactgatcct tcgcgcctgc aggaacatga ttaagctgct cacccaggat gacaccgtca
     1981 acctcagcaa gtttatctcc cgtgagcagc tctctcccac ggcagcctat cacctggtgc
     2041 acgaacaggt gattagcccg ctacacagcc acctgacacg cctgattgcc gcctggaccg
     2101 gctgcgacgc caacgatacc cgcatgatcc tccatactca tgcgttgatt ggtgagattc
     2161 tggcgttccg tcttggcaaa gaaacaattc tgttacgtac cggctggacc gcgttcgatg
     2221 aagaaaagac cgaactgatc aaccagacgg tgacctgtca tatcgacctg attttgcaag
     2281 gattatcgca aaggagtttg tagtgatgaa aaaacctgtc gtgatcggat tggcggtagt
     2341 ggtacttgcc gccgtggttg ccggaggcta ctggtggtat caaagccgcc aggataacgg
     2401 cctgacgctg tatggcaacg tggatattcg tacggtaaat cttagtttcc gtgttggggg
     2461 gcgcgttgaa tcgctggcgg tggacgaagg tgatgctatc aaagcgggcc aggtgctggg
     2521 cgaactggat cacaagccgt atgagattgc cctgatgcag gcgaaagcgg gcgtctcggt
     2581 ggcacaggcg cagtatgatc tgatgcttgc cgggtatcgc gatgaagaaa tcgctcaggc
     2641 cgccgcagcg gtgaaacagg ctcaagccgc ctatgactat gcgcagaact tctataaccg
     2701 ccagcaagga ctgtggaaaa gccgcaccat ttcggcaaat gacctggaaa atgcccgctc
     2761 ctcgcgcgac caggcacagg caacgctgaa atcagcacag gataaattgc gtcagtaccg
     2821 ttccggtaac cgtgaacagg acatcgctca ggcgaaagcc agcctcgaac aggcgcaggc
     2881 gcaactggcg caggcggagc tgaatttaca ggactcaacg ttgatagcac cgtctgatgg
     2941 cacgctgtta acgcgcgcgg tggagccagg cacggttctc aatgaaggtg gcacggtgtt
     3001 taccgtttca ctaacgcgtc cggtgtgggt acgtgcctat gttgatgaac ggaatcttga
     3061 ccaggcccag ccggggcgca aagtgctgct ttataccgat ggtcgcccgg acaagcccta
     3121 tcacggacag attggtttcg tttcgccgac tgctgaattt accccgaaaa ccgtcgaaac
     3181 accggatctg cgtaccgagc tcgtctatcg cctgcgtatt gtggtgaccg acgccgatga
     3241 tgcgttacgc cagggaatgc cagtgacggt acaattcggt gacgaggcag gacatgaatg
     3301 atgccgttat cacgctgaac ggcctggaaa aacgctttcc gggcatggac aagcccgccg
     3361 tcgcgccgct cgattgtacc attcacgccg gttatgtgac ggggttggtg gggccggacg
     3421 gtgcaggtaa aaccacgctg atgcggatgt tggcgggatt actgaaaccc gacagcggca
     3481 gtgccacggt gattggcttt gatccgatca aaaacgacgg cgcgctgcac gccgtgctcg
     3541 gctatatgcc gcagaaattt ggtctgtatg aagatctgac ggtgatggag aacctcaatc
     3601 tttacgcaga tttgcgcagc gtcaccggcg aggcacgtaa gcaaactttt gctcgcctgc
     3661 tggagtttac gtctcttggg ccgtttaccg gacgcctggc gggcaagctc tccggtggga
     3721 tgaaacaaaa actcggcctg gcctgtaccc tggtgggcga accgaaagtg ttgctgctcg
     3781 atgaacccgg cgtcggcgtt gaccctatct cacggcgcga actgtggcag atggtgcatg
     3841 agctggcagg cgaagggatg ttaatcctct ggagtacctc gtatctcgac gaagccgagc
     3901 agtgccgtga cgtgttactg atgaacgaag gcgagttgct gtatcagggg gaaccaaaag
     3961 ccctgacaca aaccatggcc ggacgcagtt ttctgatgac cagtccacac gagggcaacc
     4021 gcaaactatt gcaacgcgcc ttgaaactgc cacaggtcag cgacggcatg attcagggga
     4081 aatcggtacg tctgatcctc aaaaaagagg ccacaccaga cgatattcgc catgccgacg
     4141 ggatgccgga aatcaacatc aacgaaacta cgccgcgttt tgaagatgca tttattgatt
     4201 tgctgggtgg tgccggaacc tcagaatcgc cgctgggcgc aatattacat acggtagaag
     4261 gcacacccgg cgagacggtg atcgaagcga aagaactgac caagaaattt ggtgattttg
     4321 ccgccaccga tcacgtcaac tttgccgtta aacgtgggga gatttttggt ttgctggggc
     4381 caaacggcgc gggtaaatcg accaccttta agatgatgtg cggtttgctg gtgccgactt
     4441 ccggccaggc gctggtgctg gggatggatc tgaaagagag ttctggtaaa gcgcgccagc
     4501 atctcggcta tatggcgcaa aagttttcgc tctacggtaa cctgacggtc gaacagaatt
     4561 tacgcttttt ctctggtgtg tatggcttac gcggtcgggc gcagaacgaa aaaatctccc
     4621 gtatgagcga ggcgttcggc ctgaaaagta tcgcctccca cgccaccgat gaactgccat
     4681 taggttttaa acagcggctg gcactggcct gctcgctgat gcatgaaccg gacattctgt
     4741 ttctcgacga accgacgtcc ggcgttgatc cgattactcg ccgcgaattc tggctacata
     4801 tcaacagcat ggtagagaaa ggcgtcacgg tgatggtcac cacccacttt atggatgaag
     4861 cggaatattg cgaccgcatc ggcctggtgt accgcgggaa attaatcgcc agcggcacgc
     4921 ctgacgattt gaaagcacag tcggctaacg atgagcaacc cgatcccacc atggagcagg
     4981 cctttattca gttgatccac gactgggata aggagcatag caatgagtaa cccgatcctt
     5041 tcctggcgtc gcgtgcgggc gctgtgcgtc aaagagacgc ggcagatcgt tcgcgatccg
     5101 agtagctggc tgattgcggt agtgatcccg ctgttgctgc tgtttatttt tggttacggc
     5161 attaacctcg actccagcaa gctgcgggtc gggattttac tggaacagcg tagcgaagcg
     5221 gcgctggatt tcacccacac tatgaccggt tcgccctaca tcgacgccac catcagcgat
     5281 aaccgtcagg aactgatcgc caaaatgcag gcggggaaaa ttcgcggtct ggtggttatt
     5341 cccgtggatt tcgccgaaca gatggagcgc gccaacgcca ccgcaccgat tcaggtgatc
     5401 accgacggca gtgagccgaa taccgctaac tttgtgcagg ggtatgtcga agggatctgg
     5461 cagatctggc aaatgcagcg agcggaggac aacgggcaga cttttgaacc gcttattgat
     5521 gtacaaaccc gctactggtt taacccggcg gcgattagcc agcacttcat tatccccggt
     5581 gcggtgacca tcatcatgac ggtcatcggc gcgattctca cctcgctggt ggtggcgcga
     5641 gaatgggaac gcggcaccat ggaggctctg ctctctacgg agattacccg cacggaactg
     5701 ctgctgtgta agctgatccc ttattacttt ctcgggatgc tggcgatgtt gctgtgtatg
     5761 ctggtgtcag tgtttattct cggcgtgccg tatcgcggtt cgctgctgat tctgtttttt
     5821 atctccagcc tgtttttact cagtaccctg gggatggggc tgctgatttc cacgattacc
     5881 cgcaaccagt tcaatgccgc tcaggtcgct ctgaacgcgg cttttctgcc gtcgattatg
     5941 ctttccggct ttatttttca gatcgacagt atgcccgcgg tgatccgcac ggtgacgtac
     6001 attattcccg ctcgttattt cgtcagcacc ctgcaaagcc tgttcctcgc cgggaatatt
     6061 ccggtggtgc tggtggtaaa cgtgctgttt ttgatcgctt cggcggtgat gtttatcggc
     6121 ctgacgtggc tgaaaaccaa acgtcggctg gattagggag aagagcatgt ttcatcgctt
     6181 atggacgtta atccgcaaag agttgcagtc gttgctgcgc gaaccgcaaa cccgcgcgat
     6241 tctgatttta cctgtgctta ttcaggtgat cctgttcccg ttcgccgcca cgctggaagt
     6301 gactaacgcc accatcgcca tctacgatga agataacggc gagcattcgg tggagctgac
     6361 ccaacgtttt gcccgcgcca gcgcctttac tcatgtgctg ctgctgaaaa gcccacagga
     6421 gatccgccca accatcgaca cacaaaaggc gttactcctg gtgcgtttcc cggctgactt
     6481 ctcgcgcaaa ctggatacct tccagaccgc gccgttgcag ttgatcctcg acgggcgtaa
     6541 ctccaacagt gcgcaaattg ccgccaacta cctgcaacag atcgtcaaaa attatcagca
     6601 ggagctgctg gaaggaaaac cgaaacctaa taacagcgag ctggtggtac gcaactggta
     6661 taacccgaat ctcgactaca aatggtttgt ggtgccgtcg ctgatcgcca tgatcaccac
     6721 tattggcgta atgatcgtca cttcactttc cgtcgcccgc gaacgtgaac aaggtacgct
     6781 cgatcagcta ctggtttcgc cgctcaccac ctggcagatc ttcatcggca aagccgtacc
     6841 ggcgttaatt gtcgccacct tccaggccac cattgtgctg gcgattggta tctgggcgta
     6901 tcaaatcccc ttcgccggat cgctggcgct gttctacttt acgatggtga tttatggttt
     6961 atcgctggtg ggattcggtc tgttgatttc atcactctgt tcaacacaac agcaggcgtt
     7021 tatcggcgtg tttgttttta tgatgcccgc cattctcctt tccggttacg tttctccggt
     7081 ggaaaacatg ccagtatggc tgcaaaacct gacgtggatt aaccctattc gccactttac
     7141 ggacattacc aagcagattt atttgaagga tgcgagtctg gatattgtgt ggaatagttt
     7201 gtggccgcta ctggtgataa cggccacgac agggtcagcg gcgtacgcga tgtttagacg
     7261 taaggtgatg taacttctta tctttcgcca gcaaagacac taccgccggg ccggcaagga
     7321 ttgccggccc tgcaattgcc agcaaaaagt tgttttccag tacacgggac aacagccaca
     7381 gcacaatcag cgctgcgcca aaataccagg tcgtggtcag ctcttccagc acgtctgcca
     7441 cctcacgcca gcgttgctcg gggtggcgct gaaacaccaa catcatcact accgtgacac
     7501 aatacagtgc gcataatccc acgccgacgt gaaccagagt cttactcatc cagcccacca
     7561 ccagcagcgc cactaccagt aaatgcaaca taatctgcca gcaacttaga ccagttgcga
     7621 cacgaacacg ttgttgccac ttcatggctt ctctcctgaa gaatttatct ctacttactc
     7681 agagtaccga actttacgga aaattccgta agaccgcacc tttttgtgac gccgactaca
     7741 ctatttcaat gagataagag gcaaagacag gaaaattatg cccgatcaaa cacaacaatt
     7801 ttcgttcaag gtgctcacca tcaacattca caaaggtttt accgcgttta accgacgctt
     7861 cattttgccg gaacttcgcg acgcggtgcg taccgtcagc gccgatattg tttgcctgca
     7921 ggaagtgatg ggcgcgcacg aagttcatcc gctgcatgtg gaaaactggc ccgatacctc
     7981 gcactacgag tttctcgccg acacgatgtg gagcgatttt gcctacggtc gcaatgccgt
     8041 atacccggaa gggcatcacg gcaacgccgt actgtcgcgt tatcccattg aacattatga
     8101 gaatcgcgat gtttcggtcg atggtgcgga aaagcgcggc gtgctctact gccgcattgt
     8161 gccaccgatg accggaaaag cgattcatgt gatgtgcgta catctgggcc tgcgtgaggc
     8221 gcaccgtcag gcgcagcttg cgatgctcgc cgaatgggtg aatgagctac cggacggcga
     8281 accggtattg gtggcgggtg atttcaatga ctggcggcaa aaagctaatc atccgttaaa
     8341 agtgcaggcc ggactggatg agatttttac ccgcgcccac ggacgcccgg cgcgcacgtt
     8401 tccggtgcaa tttcctctac tacgactgga caggatctac gtcaaaaatg ccagcgccag
     8461 cgcgccaacc gcgttgccgc tgcggacatg gcgacacctt tctgatcatg cccctttaag
     8521 tgcggagatt catttatgaa atgtagctgg cgcgaaggca ataagatcca gttgctggaa
     8581 aacggcgagc aatattatcc cgcggtgttt aaggcgattg gcgaggcaca agaacgcatc
     8641 attcttgaaa cgtttatctg gtttgaggat gacgtcggca aacagctgca tgcggcacta
     8701 ctggcagcag cgcaacgcgg ggttaaagcg gaagtcttgc tggatggcta cggttcgccg
     8761 gatctcagcg atgagtttgt caatgaactg acggcagctg gcgtagtgtt ccgctactac
     8821 gatccccgcc ctcgcctttt tggtatgcgc accaatgtgt ttcgccggat gcatcgcaaa
     8881 attgtggtga tcgacgcgcg tatcgccttt attggcgggc tgaattactc cgccgagcat
     8941 atgtccagct acggtccaga ggctaaacag gattacgcgg tacgccttga agggccgatt
     9001 gtcgaagata ttctccagtt tgagctggaa aacctgcctg gacagagcgc ggcacgacgc
     9061 tggtggcgac gtcatcacaa agcggaagag aaccgccagc cgggagaagc gcaggtattg
     9121 ctggtctggc gcgataacga agaacatcgc gatgatattg aacgtcacta tctgaaaatg
     9181 ctcactcagg cgcggcgaga agtcattatc gccaacgcct acttcttccc cggctatcga
     9241 tttttacacg ccttgcgtaa agcggcacgg cgcggggtgc ggatcaaact gatcattcag
     9301 ggcgaaccgg atatgccgat tgtcagagtc ggtgcgcgct tgctgtataa ctatctggtt
     9361 aaaggcggcg ttcaggtatt tgagtaccgc cgccgcccgc tccacggcaa agtggcattg
     9421 atggacgatc actgggcgac agtagggtcc agtaatctcg atccgctcag tttgtcactg
     9481 aatctcgaag caaatgtcat catccacgat cgtcatttta accagacgct gcgcgataat
     9541 ctgaacggca ttattgccgc agattgtcag caggtcgatg agaccatgct gccgaaacgc
     9601 acctggtgga acctgaccaa aagcgtgctg gcgttccact ttttacgcca cttcccggcg
     9661 ctggttggct ggcttccggc acacacacca cgtctggcgc aggttgatcc ccccgcacaa
     9721 ccgacaatgg aaacgcagga tcgggtagaa actgaaaaca cgggggtaaa accctgatga
     9781 gtaaatcaca cccgcgctgg cgcttagcaa agaagatcct cacctggcta ttttttatcg
     9841 cggtgattgt gttgctggtg gtctacgcca aaaaagtgga ctgggaagag gtctggaagg
     9901 tcatccgcga ctacaatcgc gttgcgctgc ttagtgcggt cgggctggtg gtcgtcagct
     9961 atctgattta cggctgctat gacctgctcg cccgctttta ctgcggtcac aaactggcga
    10021 agcgccaggt gatgctggtg tcgtttatct gctacgcctt caacctgacg ctcagtacct
    10081 gggtcggcgg cattggtatg cgctatcgtt tgtactcccg actagggtta ccgggcagca
    10141 ctattacgcg gattttctcg ctcagtatta ccaccaactg gctgggctat attttactgg
    10201 cagggattat ctttaccgca ggcgtggtgg agttgccgga tcactggtat gtcgatcaaa
    10261 ctacgctgcg tattctcggc attggcttac tgatgattat cgcggtttat ttgtggtttt
    10321 gcgctttcgc gaagcaccgc catatgacca tcaaagggca aaagctggtg ctgccttcat
    10381 ggaaattcgc cctcgctcag atgctgattt ccagcgttaa ctggatggta atgggagcga
    10441 taatctggct gttacttggt caaagcgtga actatttctt tgtactgggc gtgttgctgg
    10501 ttagtagtat tgctggtgtc atcgtgcata ttccggcggg aattggtgtg ctggaagcgg
    10561 tgtttatcgc gctactggct ggggagcata catccaaagg cacaattatc gccgccctac
    10621 tcgcttatcg cgtcctgtat tactttatcc cgctgctgct ggcgctgatc tgttacctgc
    10681 tactggaaag ccaggcgaag aagctacggg cgaaaaatga agcggcgatg tgaagtttag
    10741 ttaagttctt tagtatctgc atttacgact aatgaaaaaa acgcgtatgc ctttgccaga
    10801 caagcgttat agctatcgcg gcaagtatcc caaagaagcc gataaaatag taatagagtg
    10861 ttaatgcacc tgtaacagcg caacgaaccg gactccacaa ctcttcacta tggcatttcc
    10921 ccgccaattc aaggagctta tttcgtcaat gcgagataat gcctgaccat aacacaatca
    10981 ttaagcaact tattacccaa acgggtcgtt cactcattaa tgcggcattc atgatgatca
    11041 ccagggccaa ccctgtcaac gtcatcataa tgataaaacg gtgagaacca ggatcaacat
    11101 taaataataa accggccagc atactgatta aaaacatcgc gcccgcagtg ctgaacacga
    11161 cgataatggc aggtacagga gctaaactag caacgacaat cgccaccaaa gagagtaaaa
    11221 atagatatat tacgcctgcg atgacggcat ttatcttgtt gatatcctta cttaagccat
    11281 acatacaaag taataataat cctatggcga catacatttg cggtgaatca tattttgttg
    11341 ccgtaatttc gagatacttc gctacccagg ccagtcctgc ggttgtcacc aaacctaaag
    11401 cggtaatgag ccatatgctg tttaatatcc tggcttcgtg ctgaaaatca ttagttatgt
    11461 tatttgctga gcgcgaattc attattttta tatccttaag ttaaatatca ttatctattc
    11521 caattaacgc aataaaactc gtcaattcgc actaccacac taaacccatt aataaattca
    11581 agaactgtta cagtaatcct aaagaggacg cttaaaatac taaaatagag tgttattgcc
    11641 ccaggaacga tacagcgaag agaacctgaa atttcagagg tgtcggcaac acggatcaaa
    11701 tcttccagtg ttgttgcttc acaggcagcc gtaatgcccc agataaagac agtcaaaata
    11761 ctcgctaacc acacggaaaa actgctggta aggatcaggt taacaacaaa agcaatactc
    11821 aaaccacaga aaatatatga atataactgt ctgactggtg ggataaccct attactacaa
    11881 gagcaaatca gcgccattgc ggcaaacatc cctcccgtaa ttcccattac cgtcgctatg
    11941 ctcgaaaagg tataaaggct ggcgaagaga aagccaacaa gaaggattat tagcagatag
    12001 accagacatg cgcctgatac attcagtctt ttcagggcgt tgtttaacca gagaccacct
    12061 attatcagcg cagccaaagc gaagacaaac gcacctgaga cttttgtgac gtaacccaat
    12121 gcaaatgctg aaatatccag gcacagcctg ccgtagccca tactccagct gctgttaacc
    12181 accaaatgag aagcagcatt ttgctgagcg aagcactttt ttgctgtaat tgagtagcag
    12241 tggattgtct gttcaaatgc gttatctgtc cctgaaatta cgtagtggac atagtctggc
    12301 atttgccgcg attcagttct catgtaaatg agcggaatag tctttataaa atccgaaaaa
    12361 aatcccggct ccaggccggg atcagcagga agaagagaat ttaacggcgg ttgccgaaga
    12421 tccgcaacaa catcaggaac aggttgatga agtccagata caaggttaac gcgccaagaa
    12481 tggaatattt gcgcaggttc gacgtgtcgc gggtatcaat ctgctcaccc atatttttca
    12541 gtttctgcgt gtcatacgcc gtcaatccga caaagacaat cacgccgatg taggtaactg
    12601 cccacatcaa tgcttcgctt ttcaaccaga agttgaccag cgatgccagc acaatgccga
    12661 ttaacgccat aaacagcata ttgccaaagc cacttaaatc gcgcttcgtg gtgtaaccgt
    12721 acaggctcat tgcgccgaac atcccggcag taacgacgaa agtactggcg atagaagcag
    12781 cggtatagac aatgaatata ctggaaagcg taagacccgt cagcgccgaa taaagcataa
    12841 agagcatcgt cgttacacct gcgctcagct tttgaatcat cgctgataac acaataacca
    12901 atgctaattg cgcgatgatc agaccgatta aaaagacacg gttagtgaac aacagctcca
    12961 tcacggccgc ggaattagcc gcataccagg caacaaatgc ggtcagcaac aagccaacgg
    13021 tcatccagct atagacttgc gccatataag tttgcaagcc agcccggggt tgtacgattg
    13081 aatcagaacg tgggaatctg tccatgacga tctcctgaag atataaggaa tatcttaagg
    13141 atactgcaaa atgatgaggc tgtgcatcga cgcagcgtaa acgtatgtac tgagcggtga
    13201 aattgccgga cgcagcggtg ccttatccgg ctaacaaaaa actaccagcg ttttgccgcc
    13261 tgctgatcgc tctcccgagc ttcaacccag cggtcgcctt ccggcgtggc ttcgcgcttc
    13321 cagaacggtg cgcgggtttt gagataatcc ataataaact gcccggcttc aaacgcactg
    13381 ctgcgatgcg cactggtgac accgacaaaa acgatttcat cgcccggcca taattccccg
    13441 atgcggtgaa tcacagtgac gcgccccagc ggccagcggt tacgcgcttc atcaacaatt
    13501 tctgccagtg ccttttcagt catccccgga tagtgttcga gggttaatgc tttgacgctg
    13561 tcgcccaggt tatggttgcg caccttacca gtaaaggtga ctaccgcacc gtcttcgtca
    13621 cgctccgcca gccacgggta ctcttctcct acgctgaacg gctgcggacc aacaacaatt
    13681 ttggtttctg ccatcttaac ctccggttac cggcgggaag aaagctactt cgtcgccgtc
    13741 agtcagcgga tggtcaaaac tcaccagcgt ctggttgacg gcagccagta atttgccatc
    13801 ttccagcgcc agcgcccagc gatcgctctg cgcagccatg tgctggcgta acgcttcaac
    13861 agttgggaaa tccgcagcca cttcggttgc atctgttccc accaactcgc gcacctgggc
    13921 gaaaaaaaga actttaatca tccgcttcca ccttaaagtc acccgacttg ccgccgctct
    13981 tcgccagcaa acgtaccgga ccaatcacca tatctttttg caccgctttg cacatgtcat
    14041 aaatggtcag cgccgccacg gaggccgcgg ttaatgcttc catttcgaca ccggttttcc
    14101 cggtcaggcg gcataaggtt tctatacgca cccaattgtg ctccggctcg gcctgtaaat
    14161 tgacttcaac tttgctgagc atcagcggat gacagagcgg gatcagatcc caggtgcgtt
    14221 ttgccgcctg aataccggca atacgcgcag tggcaaatac gtcgcctttg tggtggcgac
    14281 catcaataat catcgccagc gtctcgctgc gcatggtgac aaaggcttcc gcccgcgctt
    14341 cacgcacggt ttccgctttg gcggagacat ccaccatgtg cgcttcgcca gcggcgttga
    14401 tatgggtcag ttgcgacata cttatttctt caaatgtgga tggaaattac acggacgcgt
    14461 acgggcatcc agctgcggcg cgatgatatt ttcccatgcg gtacggcacg ctttggtcga
    14521 acccggcatg gcgaaaatca gcgttttgtt ggcgacgccc gctaccgcac gagattgcaa
    14581 cgtggaagtg ccaatctctt caaaagacaa catccggaac acttcaccaa aaccttcaac
    14641 ttcacggtcg aacaacggca gcaatgcttc gggagcctga tcaccttccg tcaggccagt
    14701 accacccgta atcaatacca cttgtacatc gtcgctggcg atccacgcag atacctgagc
    14761 gcgaatagcg tagcggtttt ctttcacaat ggctttatcg acaacgtgat ggcccgcttc
    14821 ttgcgccgaa tcgcgcagat agtgaccgga ggtatcgtct tcttcaccgc gacgattaga
    14881 aaccgtaaga atagcaatac gggtcgggat aaattcagtg cttacctgac tcatctgatc
    14941 tctccttttg acgttttagc cgccaatgta cgataagttt tgcgtaatac cggtgttgtt
    15001 ttgatgcagg aaatgggtct gtttcttctc ccgcagcgcc gctgaaatac gcgcttccag
    15061 cgcctgttgc tgggtatcgt cttccagcag atcgcgcagg ttaacgccgc cttcaccaaa
    15121 caggcagaga tggagtttac caatggagga aacgcgcagg cggttgcaag tggcgcagaa
    15181 gtctttttca tacggcatga taaggccaat ctctccggcg taatctggat ggcaaaagac
    15241 ttgcgcggga ccgtcgctgc gttgacgtaa ttggtggatc cagccgcgac gcagtagctc
    15301 gtcacgcaga acctgaccag agatgtgatg cttacggaag agctcgctgc cctcgcccgt
    15361 ttccatcagt tcgatgaaac gcagctggat agggcgatgc tggatccagt tcagaaaggt
    15421 gtcgagctgg tgatgattaa catcacgcat cagcacggta ttgactttga ccttctcaaa
    15481 accggcctca aatgcagcat caatccccgc catgacctgg ttgaatttat cctgcccggt
    15541 aatagcgtga aactggcggg cgtccagact gtcgacactg acgttaatgc cagtaagtcc
    15601 cgcatcgcgc cagttcgcca catcgcgttc cagacggtaa ccattggtgg tgaccgcaat
    15661 ctggcggata gcgtcgtttt cccgcacagc ggcgatgata tcggtaaagt cgcggcgtaa
    15721 agacggctca ccgcctgtca ggcgcacttt ttcggtgccc agactggcga aggcgcgcgt
    15781 aacccggcga atttcatcga cggtaagaaa gcctttattg gtgacgccgc tcggtttgta
    15841 gccatccggc aggcagtagg tgcaacgaaa gttacacaca tcggtaatcg acaggcgcaa
    15901 gtagtaaaac ttacgcgcaa atgcatcagt cagttgtgaa gccatgtaca cctttccaga
    15961 tacgggaggc gaagtcattt cttccttcgc cctggtggca atctttacga gcattgtcac
    16021 ggccaaagca ccgtatcagt tgacccaggt gcagaggcta gagtgtttag tggttatgcc
    16081 gatactagcg tgtaaatgag atttttacca tccacacttt cgctatgtaa tcatgtatat
    16141 agcgtcatga tcgtgctatt tcgctacaga ataagggaaa atggcggttt tactttgata
    16201 tacatcatca gggaggcata gcgtgcatcg tcctatagag gtagaagaaa atagtttgta
    16261 tcctcaatat tggcaggtta attgctgttt ccccgcaatt tgcgctactg tatcgcggat
    16321 aatctgactc caggaaacta tatgcgcaat cgtacgctgg ctgatcttga tcgtgtcgtt
    16381 gctctcggcg gagggcatgg actgggacgc gttctctcat cactttcgtc tttgggttct
    16441 cgtttaacgg gtatcgtcac caccacggat aatggtggct cgacggggcg tattcgccgt
    16501 tcagaaggcg gcattgcctg gggcgatatg cgcaactgcc tcaaccaact gataacggaa
    16561 ccgagcatcg cctccgcgat gtttgaatac cgttttggtg gcaatggcga actttccggt
    16621 cataatctcg gaaacttgat gttaaaggcg ctggatcacc ttagcgtgcg gcctctggaa
    16681 gccatcaatt taattcgtaa tctgctgaaa gtggatacgc atttgattcc aatgtcagag
    16741 catcccgtcg atctgatggc gattgacgat caggggcatg aagtttacgg cgaggtcaat
    16801 atcgaccagt taactacgcc gattcaagag ttattattaa cgccgaatgt acccgcaacg
    16861 cgtgaggcgg ttcacgctat caatgaagcg gatctcatca ttattgggcc tggcagtttt
    16921 tataccagcc tgatgccaat tctgctgctg aaggaaatcg cccaggcatt acgccgcacg
    16981 ccagcgccga tggtctatat cggcaatctg gggcgtgagt tgagtttacc tgcggctaat
    17041 ttgaagctgg aaagcaagct ggcaattatg gagcagtatg ttggtaaaaa ggtcattgat
    17101 gcggtcatcg tcgggccaaa agtggatgtc tcggcggtga aagagcggat tgtgattcag
    17161 gaggtactgg aggccagcga tattccgtat cgtcatgacc gccagttgtt acataacgcg
    17221 ctggaaaagg cgttacaggc tttaggttaa cggaaatccg aagaaaaatt ccggcttcct
    17281 attgaagaca aagtgcgcgt tgtttatgcc ggatgcggcg tgaacgcctt atccggccta
    17341 caaaccgcgc taattcaatg tattgcagag atcatgtagg cctgataagc gtagcacatc
    17401 aggctgtttt ccgtttgtca tcagtcttct tcgctatcct gttacgatgc ggcaataaac
    17461 aactcacgca actgatgcaa ctggtcacga atttgtgccg cttcttcgaa ctccagattc
    17521 tgcgcgtgtt gcatcatcag cccttccagt tcatggattt tctgctgcaa cgctttcggc
    17581 gacatatcca tcggcacatt atccggctca acaatcgggc gcgattttcc tctgcccttc
    17641 gctttggttt tggcaatgtt ctgccccagc gccaggatat cgaccacttt cttgttcaag
    17701 ccttgcggcg taatgccgtg ttcctcgttg tacttctgct gtttctcacg gcgacgttcg
    17761 gtttcgccaa tcgctttcgc cattgatggg gtgatcttat cgccgtagag aatcgcttta
    17821 ccgttaacgt tacgtgccgc acgaccaatg gtctggatca acgaacgttc ggaacgcagg
    17881 aagccttctt tgtcagcgtc gaggatcgcc accagcgaca cttccggcat atccagacct
    17941 tcgcgcagta agttgatccc taccagcacg tcgaactcac ccagacgcaa gtcgcggata
    18001 atctccatac gttcgacggt gtcgatatct gagtgaagat aacgcacgcg ctcgccatgt
    18061 tcttcgagat attcggtaag atcttccgcc atccgcttgg tcagtgtggt gaccagtacg
    18121 cgttcgttaa ttgccgctcg ctgacgaatc tccgaaagaa gatcatcaac ctgtgtcgcc
    18181 accggccgca cttcgataat cgggtcaagc aatccggttg gacgcaccac ctgatccacc
    18241 acatcgccgc cggatttttc cagctcgtaa ttacccggcg tcgccgaaac atagatggtt
    18301 tgcggcgcta atgcttcgaa ctcttcaaac ttaagcggac ggttatccag cgctgatggc
    18361 aggcggaagc cgtactccac cagtgtctct ttacgcgccc ggtcaccgcg atacatgccg
    18421 ccaatttgtg gaatggtgac atgagattca tcgaccacca gcagcccatc ggcagggagg
    18481 taatcaaaca gcgtcggcgg tggctcaccc ggtccacgac cggagaggaa gcgcgagtag
    18541 ttttcaatcc ccgaacagta gcccagctcg ttcatcatct ccagatcaaa ctgggtacgc
    18601 tgggtcagcc gctgctcttc cagcagtttg ttgttttcca atagcacttt gcgtctggcg
    18661 gccagctctt ctttgatctc ctccatcgcc tgtacgatgc gctcgcgcgg tgtgacgtag
    18721 tgcgttttcg ggtagatggt aaaacgtgga atggtggaaa caatctgccc ggttagcggg
    18781 tcaaataacg acaatcgttc cacttcctcg tcaaacagtt ccacgcgaag tgcaatgtca
    18841 tccgattctg ccgggaagat atcaatcacc tcgccacgaa cgcggaaagt accacgctgg
    18901 aatgcttgat cattacgagc gtattgcagc tccgccagtc ggcgcagaat cgcgcgctga
    18961 tcgataatca taccgaccgt gagatggagc atcatcttga gatataaatc aggatcgccc
    19021 agaccataaa tcgcggaaac agaggccact acaaccacat cacgccgctc cagcatcgct
    19081 ttggtggcgg ataaacgcat ctgctcaata tgttcgttaa ccgaggcatc tttctcaatg
    19141 aaagtgtcgg aactcggtac ataggcttcc ggctgatagt agtcgtagta ggagacgaaa
    19201 tactccaccg cgttttccgg gaagaactct ttcatttcgc catacagctg ggccgccagc
    19261 gttttgttgg gcgcaagtac catggttggg cgctgaaggt cagcaatgac attggcaatg
    19321 gtgaatgttt tcccggagcc agtcacgcca agtaacgtct ggtgcgccag gccatcttcc
    19381 agcccctctt cgagacgtcg aatcgcctct ggctgatcgc cagaaggttt aaaagcggaa
    19441 ttcaatttga acggtttact catgagtcgc tacctgaagg agttgggcgg gcaggtatgt
    19501 aattttactc gtcgtactta attatgccaa caaattatac tggataaaaa aacagttcat
    19561 caccataata tttctgttac agcgtaaact ccgctgtcaa tcatgagcaa gatttactct
    19621 gtggcgagat aaaactccgg ccttaccggg ttatccccaa agcaacggct ttttttaaca
    19681 tttgtcaaga tgagtgatga cagttttatg gcaagagatg cctgttcagt gactgccatt
    19741 gcaattatct aaccaattaa aaaataaaag atatttcttt gagccgtctt taacgccaat
    19801 tcgcgtgcaa gccgcgtatt ctctcgcttg cctcgtgttt tctaactcta atacacatgg
    19861 ttatccacag gaatagtgga taactgtctg cagcccctat cccccgccgc ctgggcaatt
    19921 cccacattca ccgcatcatg tggaggtaaa atttttttcg tgattatttt agaatttaat
    19981 tggttaaatt gcagtcaatc gaagactcga tctcgctcgc aatttaacca aatacaggat
    20041 ggctacaaca aggcaaggtt tatgtacttt ccggttgccg cattttctgg attttctgca
    20101 agccagggga tctctcccag cagcggcgcg ggaatcatgc gggtgagcgt ggtcatatat
    20161 tcagcgtgac gttttcccgg aggcgtaaca tcgttcgcca cccaacccgc cagagtcagt
    20221 ccggcgtgtt gtattgcctg cgcagtcaac atcgcgtgat taatacagcc gagtttcaca
    20281 ccaactacca gtatcaccgg cagttgttcc tgtgttaccc aatctgcaaa agtgaaagtg
    20341 tcagaaagcg gcgtaaacca gccgccagca ccttccacta acacccagtc agcctgttgt
    20401 tcaagcgcgc gtaatccggc gctcattacc aatgattcta tcggcctgcc ctcttgcgcg
    20461 ctgatgatgt gcggcgaggt gggttctgcg aaggtgtaag gatttaccgt tgcgtaatcc
    20521 agctgcaggc tgctgttgtg ctgtaacgcc agcgcgtcac tattacgtaa gccttccggg
    20581 gtcttttcgc tgccagaggc gaccggttta taacctgccg tccgataacc tactgccttc
    20641 gcggcttgta aaagtgcaca actggcgacg gttttcccca cttcggtatc cgttccggtg
    20701 acaaaataac gtttactcac gagcaatcac tcccaaaaaa agatgatacg tcagaggata
    20761 tcgcccctgt tgttgcggcc aggccagttg caatcgctgc aattgcgaac gcgataatat
    20821 tcgcggatcg cgcccttcat gaagatgcgt agcaccgatg cctttcagcg aacgcatggc
    20881 actgagcgca tcatcaaacc atagtgtgat gggctgaata tgatgttgat aatgcacgcc
    20941 gtttagcgac tgttcgattt catctggcgg taaaaagcga ttagcatgcg gacgctcgtc
    21001 caccgcctgc cacgcctgat gcagttcggg taacgatccc tgcaccagcg tggtaaacgc
    21061 caccacgcct ccggggcgca ccacccgata cagctcgcgg agtgccgtgg ataaattacc
    21121 gcaccactgc actgcgagat tgctccatgc aagatcgaac gtcgcagtcg ctaacggcag
    21181 ggattcgata tctcccgcca gataatggtc tgcagcatct ttctggcggg cttgcacaag
    21241 cattggcggt gagagatcta acgccgtcac cagtgcgtga cgctctcgcc agtggcggct
    21301 catccagcct ggcccacacc ccgcgtccag tacgtgggtg tatttacgct gtgggagcat
    21361 tgccagtaag acgtcagcac tccggcgctg taactctgcg tgttgctcat agtgcgcggc
    21421 tgcccgacca aatgccgctg caatggcttg tttattaacc gttgccatgc agcacctcca
    21481 gcagacggtc gatatcctgc atttcatgcg cagcggttag cgttaagcgc agtcgcgcag
    21541 taccagcggg tacggttggc gggcgaatcg ccgtgaccca gcagccttgc tggcgcagtt
    21601 tttctgccag ttgtaacgca cggctgttat caccgacgat caatggctgg atggcgctgc
    21661 atgaatcagc cagcgtaaac ggcaaatcct gtactccggc acgaaaacgc gcaatgagtg
    21721 acaccagttt ttcgcgccgt gcatccccct catcacggcg aatgaccgcc agcgacgcac
    21781 gtaatgcctg cgcctgagcg ggcggcatac tggtgctgta gataaggtgg cgggcgaatt
    21841 gcagcagata atccgccacc gtactggagc aaagcaccgc agccccactg acgccaaatc
    21901 ctttgccaaa agtcaccacc agcaattctg gttttacctt ttgcagccag cagctgccgc
    21961 gcccctgctc cccgataacg cccgtgccgt gggcatcatc gaccatcaac cagccattgt
    22021 gctgttgcgt tacctgctgg atttccgcca gtggcgcaat atcgccgtcc atgctgaaca
    22081 cgccttctgt cactaccagt tgctgccccg gacaggggga agcaagcagt cgcgccagat
    22141 gagtgacatc gttatgagta aaacggcgaa gctgcgacgg gcttaaactg gcagcctcca
    22201 gcaatgaggc atggctaagc cggtcagcga caatgcggtc ctcttttgcc atcatcgcgg
    22261 caatgaccgc ctgattagcg gcaaaaccag agataaatag cagtgcccgc gaatagccca
    22321 gccactcggc cagttcttct tccagcgcct gatgcgcaac gctataaccg ctgacgtgac
    22381 cggaaccgcc gctaccgacg ccaaattgct ccgcaccctg ctgccaggca cggataattt
    22441 gcggatgatg gcttaaacct aaataatcgt tactggaaaa gttcagatac tggcgatcat
    22501 ccgccaccag ccagcgtccg gctccttgcg ccactggata acggcgacgc agggcatcgg
    22561 cagcacgccg cgcatcgagc gccgcgttga ttttctcctg ccagctcata gtgctgccgc
    22621 gttgtaatat tcgtcagtgt ccggggtcat cagtgcctgc tccagacgct gctgttgttc
    22681 gttatcccct gccagcacgg cagtttgctg cggatttagc cccagtttgc gaaacagttg
    22741 caggtcttta tcttcttccg gattcggcgt ggttagcagt ttgcaaccgt agaaaatcga
    22801 gtttgcgcct gccataaagc acatcgcctg agtctgttcg ttcatctgct cgcgtccggc
    22861 agaaaggcgc acgtaagagg ttggcatcat gatccgcgcg accgcaatgg tgcgaataaa
    22921 atcaaaggca tcgacatcat cgttatcggc aagcggcgtg cctttcacct tcaccagcat
    22981 gttgattggt acgctttccg gcggcgtcgg caggtttgcc agttgcagca ataatccggc
    23041 gcgatctttt accgtttcgc ctaagcccac aatgccgcca gaacagactt tgatcccggc
    23101 atcgcgcact ttttccagcg tatcgaggcg ttcctgataa gtgcgggtgg tgatgatatt
    23161 gccgtaaaac tccggcgagg tgtccaggtt gtggttgtag taatccagcc cggcgttcgc
    23221 gaggcgctgc gcctgagatt cactcaacgt gcccagcgtc atacacgcct ccagccccat
    23281 cgcttttacc ccctgcacca tttgttccag gtacggcata tcgcgttcgt ggggattctt
    23341 ccacgccgcg cccatacaga agcgcttcga tcctgccgct ttcgctttgc gcgccgactc
    23401 cagcacctgt tcaacttcca tcaaccgctc ggcttccagc ccggttttgt agcgcgagct
    23461 ttgcgggcag tatttgcaat cttccggaca agctccggtt ttaatcgaca gcaacgtgct
    23521 gacctgtacc tgacgaggat cgaaatgctg acgatgcacc tgctgcgctt caaacagcag
    23581 atccagcaac ggtttttcaa ataattctgt gacttgcgac aatgtccagc gtgggcggtg
    23641 agccatgggg cttctccaaa acgtgttttt tgttgttaat tcggtgtaga cttgtaaacc
    23701 taaatctttt caatttggtt tacaagtcga ttatgacaac ggacgatctt gcctttgacc
    23761 aacgccatat ctggcaccca tacacatcca tgacctcccc gctgccggtt tatccggtgg
    23821 tgagcgccga aggttgcgag ctgattttgt ctgacggcag acgcctggtt gacggtatgt
    23881 cgtcctggtg ggcggcgatc cacggctaca atcacccgca gcttaatgcg gcgatgaagt
    23941 cgcaaattga tgccatgtcg catgtgatgt ttggcggtat cacccatgcg ccagccattg
    24001 agctgtgccg caaactggtg gcgatgacgc cgcaaccgct ggagtgcgtt tttctcgcgg
    24061 actccggttc cgtagcggtg gaagtggcga tgaaaatggc gttgcagtac tggcaagcca
    24121 aaggcgaagc gcgccagcgt tttctgacct tccgcaatgg ttatcatggc gatacctttg
    24181 gcgcgatgtc ggtgtgcgat ccggacaact caatgcacag cctgtggaaa ggctatctgc
    24241 cagaaaactt gtttgctccc gccccacaaa gccgtatgga tggcgaatgg gatgagcgcg
    24301 atatggtggg ctttgcccgc ctgatggcgg cgcatcgtca tgaaatcgcg gcggtgatca
    24361 ttgagccgat tgtccagggc gcaggcggga tgcgcatgta ccatccggaa tggttaaaac
    24421 gaatccgcaa aatatgcgat cgcgaaggta tcttgctgat tgccgacgag atcgccactg
    24481 gatttggtcg taccgggaaa ctgtttgcct gtgaacatgc agaaatcgcg ccggacattt
    24541 tgtgcctcgg taaagcctta accggcggca caatgaccct ttccgccaca ctcaccacgc
    24601 gcgaggttgc agaaaccatc agtaacggtg aagccggttg ctttatgcac gggccaactt
    24661 ttatgggcaa tccgctggcc tgcgcagcag caaacgccag cctggcgatt ctcgaatctg
    24721 gcgactggca gaagcaggtg gcagctattg aagtacagct acgcgagcaa ctggcacccg
    24781 cccgtgatgc cgaaatggtt gccgatgtgc gcgtactggg ggccattggc gtggtcgaaa
    24841 ccactcatcc ggtgaatatg gcggcgctgc aaaaattctt tgtcgaacag ggtgtctgga
    24901 tccggccttt tggcaaactg atttacctga tgccgcccta tattattctc ccgcaacagt
    24961 tgcagcgtct gaccgcagcg gttaaccgcg cggtacagga tgaaacattt ttttgccaat
    25021 aacgagaagt ccgcgtgagg gtttctggct acactttctg caaacaagaa aggagggttc
    25081 atgaaactca tcagtaacga tctgcgcgat ggcgataaat tgccgcatcg tcatgtcttt
    25141 aacggcatgg gttacgatgg cgataatatt tcaccgcatc tggcgtggga tgatgttcct
    25201 gcgggaacga aaagttttgt tgtcacctgc tacgacccgg atgcgccaac cggctccggc
    25261 tggtggcact gggtagttgt taacttaccc gctgataccc gcgtattacc gcaagggttt
    25321 ggctctggtc tggtagcaat gccagacggc gttttgcaga cgcgtaccga ctttggtaaa
    25381 accgggtacg atggcgcagc accgccgaaa ggcgaaactc atcgctacat ttttaccgtt
    25441 cacgcgctgg atatagaacg tattgatgtc gatgaaggtg ccagcggcgc gatggtcggg
    25501 tttaacgttc atttccactc tctggcaagc gcctcgatta ctgcgatgtt tagttaatca
    25561 ctctgccaga tggcgcaatg ccatctggta tcacttaaag gtattaaaaa caactttttg
    25621 tctttttacc ttcccgtttc gctcaagtta gtataaaaaa gcaggcttca acggattcat
    25681 ttttctattt catagcccgg agcaacctgt gaacacattt tcagtttccc gtctggcgct
    25741 ggcattggct tttggcgtga cgctgaccgc ctgtagctca accccgcccg atcaacgtcc
    25801 ttctgatcaa accgcgcctg gtacctcttc tcgcccgatt ctgtcggcaa aagaagcgca
    25861 gaatttcgat gctcaacact attttgcatc cctgacacca ggtgctgcag cgtggaatcc
    25921 ttccccgatt accctgcctg cgcaacctga ctttgttgtc ggcccggcgg gcactcaagg
    25981 tgtaacgcat accacgattc aggcggcggt agatgcggca attatcaagc gtaccaacaa
    26041 gcgccagtat attgccgtga tgcctggtga gtatcaggga acggtatatg tccctgccgc
    26101 tccgggtgga attactctgt acggtacagg tgaaaaaccg attgatgtga agattgggct
    26161 ttcccttgat ggtggcatga gccctgccga ctggcgtcac gacgtcaacc cgcgcggcaa
    26221 atatatgcca ggtaaaccag cgtggtatat gtacgatagc tgccagagca aacgcagcga
    26281 cagtatcggt gttctctgct ctgcggtctt ctggtcacaa aacaatggcc tgcaactgca
    26341 aaatctgacc atcgaaaaca cgctgggcga tagcgtagat gcaggtaacc atccggcggt
    26401 ggcactgcgt actgatggtg accaggtaca gattaacaac gttaacattc tcggtcgtca
    26461 gaacaccttc tttgtcacca acagcggtgt gcagaaccgt ctggaaacga atcgtcagcc
    26521 gcgtacgctg gtgaccaaca gctacattga aggggatgtg gatatcgttt ctggtcgcgg
    26581 cgcagtggtg ttcgataaca ccgaattccg cgtggtgaac tcacgtactc agcaagaagc
    26641 gtatgtgttt gcaccggcta cgctgtccaa catttactac ggtttcctcg ccgtaaacag
    26701 ccgtttcaat gctttcggtg atggtgtggc gcaactgggc cgctcgctgg atgttgatgc
    26761 caataccaac ggtcaggtgg tgatccgtga tagcgccatc aacgaaggtt ttaacacggc
    26821 taaaccgtgg gccgatgcgg tgatctctaa tcgtccgttt gcgggtaata ccggcagcgt
    26881 aaatgataac gacgaaatac agcgcaatct gaatgacact aactacaacc gcatgtggga
    26941 atacaataac cgcggcgtgg gtagtaaagt ggttgcagag gcgaagaagt aagagcaatt
    27001 aactatttgc cggatgcggc gtaaacgcct tatccggcct acggttcgat gcgatttgta
    27061 ggtcggataa gatgcgcaag catcgcatcc gacaataagt gccggatgct gcgaaaatgc
    27121 cttatctggc ctacagattc gatgcgattc gtaggtcgga taagatgcgc aagcatcgca
    27181 tccgacaata agtgccgaat gcgacctaca ttcacatggc gctttttaca tctgacggtt
    27241 tttattgaag ttaatcaaac tacccgcctt gataatctcg cgctcttcag cagtcagact
    27301 ttccatatag agcgtaattt ccgttaccgg cgcatcttca tggatcacat aacctttaaa
    27361 cgtcgtaccc ggattatcca gcgccgcttt aatgccaggg atgtaaatgt aatcccccac
    27421 ttcaaaggtt ggcacttccg ccatctgcag cggtaacatc ccccagttga tgacgttaga
    27481 acggtagcgt ttagtcgcgt actcttcggc aatattcgcc agaccgccaa tcacacgctg
    27541 gcagctcgcc gcctgttcac gcgcagaacc atcgcctggt ttcaccgcat ataccatgct
    27601 gccaatttca gtttgcagcg gatcaatatg ctcctgacca gcaatctgct taatgcgcgc
    27661 aaacacctct gtcagctcgc tgacattccc cgccagacgc tgattttcca gctcagcagt
    27721 cgctttactt ctgccaacat aaccgggatc gcggcgtgac agggtaaact ccgccagacc
    27781 aatcggattt gaacgataag aagaggtttc accggaagga atcagttcgt cggtggtggt
    27841 cacttcgtcg aggatcttcg agcacacttt caggacgata ttgtcagtca gcgcacccaa
    27901 ttccggccag tctttaatgt tcggtccgta aatcagcggt tgctgagttg cccctttcac
    27961 aaagccctga taaacacggt ttttatacgg cgttacatcg aaggcgtact ccggcacgtt
    28021 gtcccagcaa tcaagttcgc tggcagaggt taaatagcca ccgtttgccg cagtcgcagc
    28081 gatagaacga gcgtccatca acgccaccgc tgacatctgc ccattagctg gcttagagcc
    28141 ttcgcggttc gggaagttac gcgtggtgtg gcgaatactc aaaccgttgt tgattggcgt
    28201 atcgcccgcg ccaaagcatg ggccgcagaa cgcggttctg atgattgcgc ctgcgccaat
    28261 caaatctgct accacacctt ttttggcgag atccataaac accggctgtg atgacgggta
    28321 aactgccagc gagaaggtgt cattgccaca ggattgaccg cgcagtgcat tcgccgccgc
    28381 gatgacgttt tcgtagttac cgccagaaca gcccgcgata atcccctgct gcactttcag
    28441 gcgaccattt tccactttat ccagcagcga gagtttggct ttaccgtgcg ccacgcgttc
    28501 ggactcaatt tcaatctcac gcagaatgtc ggtcaggttc tggttcagtg tgtcgatttc
    28561 atacacgttg ctcgggtgga acggcagcgc aatcattggt ttgatggcgc ttaaatcgac
    28621 gctgatgcag ccatcgtagt acgccatcgg ttgagggtta agctggcagt aatcctggcc
    28681 gcgaccgtgc agcgccagcc agttatgcac ttcttcatca gtttgccaga cggagcttaa
    28741 acaggtggtt tccgtggtca tcacgtcaac gctgttacgg aaatcggtag agagcgcgct
    28801 aacgcccggt ccaacgaact ccatgacttt gtttttgacg taaccgtttt tgaacaccgc
    28861 gccaatgata gccagcgcca catcctgtgg ccccacatac ggcgcaggtt ttccggtcag
    28921 atgcaccgca accacgcccg gatagtcgat atcccaggtg tcattaagca gctgttttac
    28981 caactcaccg ccgccctcac cgactgccat tgtccctaat gcaccgtaac gggtgtggct
    29041 gtctgacccg aggatcattt tgccgccgcc tgccatcatc tcacgcatat attgatggat
    29101 gaccgcaatg tgcggaggca caaaaatacc gccataacgc tgggccgccg ataaaccaaa
    29161 aacatggtca tcaccgttaa tagtgccgcc gacggcgcag agtgagttat ggcagttggt
    29221 cagcacatac ggcagcggga aacgttccat accggacgct ttagcggtct gtacaatacc
    29281 aacaaaggta atatcgtgag aggctaatga atcaaactta attttaagtt tatccatatt
    29341 tccggacgta ttatgcgaag agagaataga ccaggcaata gtgccttttt tggcttcttc
    29401 ttttttaatt tcgccggtga aatgttcttc ggcaattatt tcgttattac tggcgagaaa
    29461 cacgcctttt tcagataact tgatcatcaa atgctccaga tatctgcgca ccgtgaagtg
    29521 cgcagaaaga taataaatgg atatactcta aataattcga gttgcaggaa ggcgacaagc
    29581 gagtgaatcc ccaggagctt acataagtaa gtgactgggg tgagcgaacg cagacgcagc
    29641 acatgcaact tgaagtatga cgagaatatt tacagcatgt tccagccgat cagcatattc
    29701 caccaccaca cgccgagggt gatatgcacc aggaaggtta atatcgtcag taccgcaccg
    29761 accaaccacc aggatttaat atcgttataa cccacgccaa agatgaccgg acctgccgcg
    29821 ccgccatagt gagtaaccat gccgccatag gagttggaga acaacagtgc cagcgcggtt
    29881 aacattaacg gtgcgccgga gacgttcgcc agcatggcaa ataccggtaa catagcaacg
    29941 atataggcac taccggaagc gaagaaataa cgcacgataa tgctgaggaa aataataacg
    30001 aagaaagcaa cgttaccgtg accatcaaat gccaggttat ttttaaagac ttcagctaac
    30061 cattcgaaga atttaacttt cgataataag gagcttaatc cgataatacc gccgtaccag
    30121 attaaggtat tccagccgcc tttattttta accacgtctt cccaggtaac gatacccagc
    30181 agcagcatgg tcgccataac aacgattgcc acagtggatt catcaacccc cagagactta
    30241 ctgaaaatcc agcccagcag cgccagcaca aagacaccga gcagcatttt ttcgcggatt
    30301 ttcatcggtc ccagttcggc aaggcccgct ttagcgatgg ttttattatc caccttctta
    30361 atttctggcg gatacatggt ataaatcacc agcggggtga ccatcagcat aatgatgccc
    30421 ggcaatccgg cggccagtgc ccatccaccc cagctaattt gcaggtgcag aatgtcgttg
    30481 atcattttca gcgccagaat gttccccgcc attgcggtaa agaacatata gctggtggtt
    30541 ttggtgacca tgtaaatgga catcatcagg taatgtccga cacgacgcgg acttttttcc
    30601 ggttcggacc ccaaagccac cgccacgctg ttgatgatcg gtaacacaat accgcccgca
    30661 cgcgcggtgt tagacggtgt tgccggagcc agtaccagat cgaggaatac cgtaacgtaa
    30721 cccagaccca gcgtggtgtt accgatttta ccaatcagca gataggcaat acgtttacct
    30781 aaaccggtgg tcacaaatgc ggcgcttaag gtaaacgccg agaacaccag ccaggtagta
    30841 cctgaagagt aaccgcttaa tacggcggtg gtcttaaacg caccgtcgga taagttaccg
    30901 accaccacca tggaggcagc aacggcaatt aacagtacga caggttccgg gaaaggcttg
    30961 ataaccagcc ccacaatggc cgccaggtaa ataccaaaaa gcacccacgc cagttcgctt
    31021 aatcctgccg gagctggcat aaaaccaata atacatggga tcgctaatat cagaattagc
    31081 ttccataacg atttcttatt catttaaaat cctgttcttt ctgtatgact catacagaag
    31141 tcctttattg attgtcctta tcctgacaac gaattttttc agggaagata aacttcaccg
    31201 gaaaatattt ttctggtcgt ccgaataaca gatgcgcgta acgtcgtggc atcctgacct
    31261 tcattactta aatgaacgtc gagcgcacca ctggggtgtt caatattgat attgccgtat
    31321 cctacagaag ggacgatttg tcgggtgacg gtgccttcca atgcacaact actggaaata
    31381 gcaatagcac cggttatcgc cagcgcgcga tggcaagaat gcggcataaa ataacgtaca
    31441 ttaattgccc cgcctttctg cgctggagaa ataagcacag gtttagggat aaccatatta
    31501 ctgacatcac ctaagcccat tgctttaccc gcttgtagac ggatagattc aatgcgtgct
    31561 aataatgctt tgtcggcatc cagttccgcc ggtaattcat aacctgtttt tcccagatat
    31621 tcagccggaa taatgacgac tggcatcgcc atatcgatac aggtcaccgg gacatcgtca
    31681 aaataatcaa tctgattatc agtcgggaag acttttccgg ttttggttcc ggcggcattc
    31741 aggaaagtga gcgcaaccgg tgcggcagta cccggtacgc cgtcaattct ggcgctaccc
    31801 tcgtactcga caacaccatt tggcgtttgc acatcagctt cgatgaacgt accagtattg
    31861 acgttgcgga tacgtacgcg ggtaactggt gaagtcgctg caatcaaacc attttcaatg
    31921 gcgaatgccc caacaccaga cagcatattg ccacaattag gcgtggtatc gacacgttgc
    31981 tcatggacaa taacttgagc aaacagataa tcgacatcag cacgcggatc gctggaacgg
    32041 ctaataatgg cgactttact ggtcagcgga ttaccgccgc caataccgtc aatttccaga
    32101 tcgttaccgg aacccataat tgccatcaat attttatcgc gctgcgtttg atcttcgggt
    32161 aaatgttccg ctaacaggaa cgcgcccctc gaggttccac ctcgcatcat cacgcagggt
    32221 atttttttca taataatgcc ctcaatttaa aaacataact tattgcgata tggttacatt
    32281 aaggaca
//
LOCUS       DAZKIV010000037        31581 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZKIV010000037 DAZKIV010000000
VERSION     DAZKIV010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.037"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2895
                     /locus_tag="ACHOT6_001733"
     CDS             <1..2895
                     /locus_tag="ACHOT6_001733"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9946965.1"
                     /translation="LVVLLTPGDDPLRAFLIWVLSESIGALALVPLGLLFKPHYLLRH
                     RNPRLLFESLLTLAITLTLSWLSMLYLPWPFTFIIVLLMWSAVRLPRMEAFLIFLTTV
                     MMVSLMMAADPSLLATPRTYLMSHMPWLPFLLILLPANIMTMVMYAFRTERKHISESE
                     TRFRNAMEYSAIGMALVGTEGQWLQANKALCQFLGYSQEELRGLTFQQLTWPEDLNKD
                     LQQVEKLISGEINTYSMEKRYYNRNGDVVWALLAVSLVRHTDGTPLYFIAQIEDINEL
                     KRTEQVNQQLMERITLANEAGGIGIWEWELKPNIFSWDKRMFELYEIPPHIKPNWQVW
                     YECVLPEDRQHAEKVIRDSLQSRSPFKLEFRITVKDGIRHIRALANRVLNKEGEVERL
                     LGINMDMTEVKQLNEALFQEKERLHITLDSIGEAVVCIDMAMKITFMNPVAEKMSGWT
                     QEEALGVPLLTVLHITFGDNGPLMENIYSADTSRSAIEQDVVLHCRSGGSYDVHYSIT
                     PLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSASHDALTHLANRASFEKQLRILLQTV
                     NSTHQRHALVFIDLDRFKAVNDSAGHAAGDALLRELASLMLSMLRSSDVLARLGGDEF
                     GLLLPDCNIESARFIATRIISAVNDYHFIWEGRVHRVGASAGITLIDDNNHQAAEVMS
                     QADIACYASKNGGRGRVTVYEPQQAAAHSERAAMSLDEQWRMIKENQLMMMAHGVASP
                     RIPEARNLWLISLKLWSCEGEIIDEQKFRRSFSDPALSHALDRRVFHEFFQLAAKAVA
                     SKGISIALPLSVAGLSSATLVNDLLEQLENSPLPPRLLHLIIPADAILDHAESVQKLR
                     LAGCRIVLSQVGRDLQIFNSLKANMADYLLLDGELCANVQGNLMDEMLITIIQGHAQR
                     LGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIADAQPLDLLVNSSYFAIN"
     gene            complement(2934..3791)
                     /gene="alkA"
                     /locus_tag="ACHOT6_001734"
     CDS             complement(2934..3791)
                     /gene="alkA"
                     /locus_tag="ACHOT6_001734"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFP9946966.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDMARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGTLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            3925..5277
                     /gene="yegD"
                     /locus_tag="ACHOT6_001735"
     CDS             3925..5277
                     /gene="yegD"
                     /locus_tag="ACHOT6_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9946967.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(5289..7229)
                     /gene="yegI"
                     /locus_tag="ACHOT6_001736"
     CDS             complement(5289..7229)
                     /gene="yegI"
                     /locus_tag="ACHOT6_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFP9946968.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(7226..7987)
                     /gene="pphC"
                     /locus_tag="ACHOT6_001737"
     CDS             complement(7226..7987)
                     /gene="pphC"
                     /locus_tag="ACHOT6_001737"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFP9946969.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(7984..8643)
                     /gene="yegL"
                     /locus_tag="ACHOT6_001738"
     CDS             complement(7984..8643)
                     /gene="yegL"
                     /locus_tag="ACHOT6_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9946970.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNTGL
                     VTFRDELLADSLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(8864..8923)
                     /gene="ibsA"
                     /locus_tag="ACHOT6_001739"
     CDS             complement(8864..8923)
                     /gene="ibsA"
                     /locus_tag="ACHOT6_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFP9946971.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(9196..9252)
                     /locus_tag="ACHOT6_001740"
     CDS             complement(9196..9252)
                     /locus_tag="ACHOT6_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9946972.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            9531..10778
                     /gene="mdtA"
                     /locus_tag="ACHOT6_001741"
     CDS             9531..10778
                     /gene="mdtA"
                     /locus_tag="ACHOT6_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFP9946973.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLIALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            10778..13900
                     /gene="mdtB"
                     /locus_tag="ACHOT6_001742"
     CDS             10778..13900
                     /gene="mdtB"
                     /locus_tag="ACHOT6_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFP9946974.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFTLAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            13901..16978
                     /gene="mdtC"
                     /locus_tag="ACHOT6_001743"
     CDS             13901..16978
                     /gene="mdtC"
                     /locus_tag="ACHOT6_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFP9946975.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIVISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            16979..18394
                     /gene="mdtD"
                     /locus_tag="ACHOT6_001744"
     CDS             16979..18394
                     /gene="mdtD"
                     /locus_tag="ACHOT6_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9946976.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            18391..19794
                     /gene="baeS"
                     /locus_tag="ACHOT6_001745"
     CDS             18391..19794
                     /gene="baeS"
                     /locus_tag="ACHOT6_001745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFP9946977.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            19791..20513
                     /gene="baeR"
                     /locus_tag="ACHOT6_001746"
     CDS             19791..20513
                     /gene="baeR"
                     /locus_tag="ACHOT6_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFP9946978.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            20704..21036
                     /gene="yegP"
                     /locus_tag="ACHOT6_001747"
     CDS             20704..21036
                     /gene="yegP"
                     /locus_tag="ACHOT6_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFP9946979.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            21245..21541
                     /locus_tag="ACHOT6_001748"
     CDS             21245..21541
                     /locus_tag="ACHOT6_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFP9946980.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            21543..21839
                     /locus_tag="ACHOT6_001749"
     CDS             21543..21839
                     /locus_tag="ACHOT6_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9946981.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            21942..23303
                     /gene="yegQ"
                     /locus_tag="ACHOT6_001750"
     CDS             21942..23303
                     /gene="yegQ"
                     /locus_tag="ACHOT6_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFP9946982.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     VEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(23634..23951)
                     /gene="yegR"
                     /locus_tag="ACHOT6_001751"
     CDS             complement(23634..23951)
                     /gene="yegR"
                     /locus_tag="ACHOT6_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFP9946983.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            24357..25256
                     /gene="yegS"
                     /locus_tag="ACHOT6_001752"
     CDS             24357..25256
                     /gene="yegS"
                     /locus_tag="ACHOT6_001752"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFP9946984.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(25338..26111)
                     /locus_tag="ACHOT6_001753"
     CDS             complement(25338..26111)
                     /locus_tag="ACHOT6_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9946985.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(26217..27257)
                     /gene="gatD"
                     /locus_tag="ACHOT6_001754"
     CDS             complement(26217..27257)
                     /gene="gatD"
                     /locus_tag="ACHOT6_001754"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9946986.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(27305..28660)
                     /locus_tag="ACHOT6_001755"
     CDS             complement(27305..28660)
                     /locus_tag="ACHOT6_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFP9946987.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(28664..28948)
                     /gene="gatB"
                     /locus_tag="ACHOT6_001756"
     CDS             complement(28664..28948)
                     /gene="gatB"
                     /locus_tag="ACHOT6_001756"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFP9946988.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            complement(28979..29431)
                     /gene="gatA"
                     /locus_tag="ACHOT6_001757"
     CDS             complement(28979..29431)
                     /gene="gatA"
                     /locus_tag="ACHOT6_001757"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFP9946989.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(29441..30703)
                     /gene="gatZ"
                     /locus_tag="ACHOT6_001758"
     CDS             complement(29441..30703)
                     /gene="gatZ"
                     /locus_tag="ACHOT6_001758"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFP9946990.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(30732..31580)
                     /gene="gatY"
                     /locus_tag="ACHOT6_001759"
     CDS             complement(30732..31580)
                     /gene="gatY"
                     /locus_tag="ACHOT6_001759"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFP9946991.1"
                     /translation="MVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAPVI
                     IAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVMID
                     ASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQARE
                     FAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDIQQ
                     TIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIADC
                     GCEGRA"
BASE COUNT         7600 a         8361 c         8063 g         7557 t
ORIGIN      
        1 ctggttgtcc tgctgacgcc cggagacgat cctctcaggg catttttgat atgggtactg
       61 tcagaatcca tcggcgctct ggcactggtg ccgctgggat tgttatttaa accacactat
      121 ctgctgcgcc atcgcaaccc acggttgctt tttgagtcgc tgctcacgtt agccatcaca
      181 ctgacgttaa gctggctttc gatgctgtat ctgccgtggc cttttacttt cattattgtg
      241 ctgttgatgt ggagcgccgt gcgtctgcca cgaatggaag cctttttgat cttccttacc
      301 acggtgatga tggtgtcgct gatgatggcc gcggatccct ccctgcttgc tacgccgcgt
      361 acgtacctga tgagccatat gccgtggcta ccgtttttgc tgatcctgct gcccgccaac
      421 atcatgacca tggtgatgta cgcatttcgt actgaacgaa agcatatttc cgaaagcgaa
      481 acccgttttc gcaacgcgat ggagtattcc gccatcggca tggcccttgt gggaacggaa
      541 ggacaatggc tgcaagccaa taaagcctta tgtcagtttc tcggttacag tcaggaagag
      601 ctgcgcggac tcacctttca gcaactgacc tggccggagg atctcaataa agatctccaa
      661 caggttgaaa agctgataag cggtgaaata aacacctatt caatggaaaa acgctactac
      721 aaccgtaatg gcgatgttgt ctgggcgttg cttgccgtct cactggtgcg ccacacggat
      781 ggcacgccgc tctattttat cgctcagatt gaagacatta acgagctaaa acgcaccgaa
      841 caggtgaatc agcaactgat ggaacgcatc acgctggcta acgaagcggg cgggattggc
      901 atctgggagt gggagttgaa gccgaatatt tttagctggg ataagcggat gttcgagctg
      961 tatgaaattc ctccacatat caaaccgaac tggcaggtgt ggtacgagtg cgtgctgccg
     1021 gaagatcgcc agcatgccga aaaagtgatt cgtgattcgt tgcaatcacg ctcgcccttt
     1081 aaactggaat ttcgcattac cgtaaaagac ggtattcgcc atatccgcgc cctcgccaac
     1141 cgggtactga ataaagaagg cgaagtcgaa cgcctgctcg gcattaatat ggatatgact
     1201 gaggttaaac agcttaacga ggcattgttt caggaaaaag agcgcctgca cattacgctg
     1261 gactccatcg gcgaagccgt ggtctgtatt gatatggcaa tgaaaattac ctttatgaat
     1321 ccagtggcgg agaagatgag cggctggacg caggaagaag cattaggtgt tccgctcctg
     1381 acggtgttgc atattacctt tggcgacaat ggtcccctga tggagaacat ttacagtgcc
     1441 gacacctcac gttccgcgat tgaacaagat gtggtgttgc actgtcggag cggcggcagc
     1501 tacgacgtgc attacagtat tacgccgtta agtactctgg acggcagcaa tattggttcg
     1561 gttctggtga ttcaggacgt taccgaatca cgcaaaatgc tgcgccagct gagctacagc
     1621 gcctcccatg atgcactgac gcatctcgcc aaccgcgcca gttttgagaa acaactgcgt
     1681 atcctgctgc aaacggtaaa cagtacacat cagcgacatg ccctggtgtt tatcgatctt
     1741 gatcgcttta aagcggtgaa tgacagcgcc gggcatgcgg cgggtgacgc tttactgcgc
     1801 gaactggcgt cgttgatgct gagtatgctg cgctccagcg acgtgctggc gcgactcggt
     1861 ggtgatgaat ttggtctact gttgccagac tgtaatatcg aaagtgcgcg ttttatcgct
     1921 acacgcatta tcagtgccgt gaatgattat cactttatat gggaaggccg tgtgcatcgg
     1981 gtaggtgcca gtgccgggat taccttgatt gatgacaaca atcatcaggc ggcagaagtg
     2041 atgtcgcagg ctgatatcgc ctgttatgcc tccaaaaatg gtggacgggg ccgggtgacg
     2101 gtttacgaac cacagcaagc tgccgcacat agcgaacggg cggcgatgtc gcttgatgaa
     2161 cagtggcgga tgattaaaga gaatcagttg atgatgatgg cccacggtgt cgcttcgccg
     2221 cggatcccgg aagcgcgtaa tttgtggctg atttcgctta aactctggag ttgcgaaggc
     2281 gagattattg atgaacaaaa atttcgtcgt agcttcagcg atccggcact tagccatgct
     2341 cttgaccgac gggtattcca cgaatttttc cagctggccg caaaagcggt tgccagtaaa
     2401 ggcataagca tcgccctccc cctttccgtt gccggtttga gtagcgccac gctggtgaat
     2461 gatctgcttg agcagctgga aaatagccct ctaccaccac ggttattaca tctgattatt
     2521 ccggcagacg cgattttaga tcacgcagaa agcgtgcaaa aactgcggct ggcgggatgt
     2581 cggatagtgc tcagccaggt gggccgcgat ctgcaaatct tcaactcgct gaaagcgaat
     2641 atggcagatt acctgctact tgatggtgag ttatgcgcca acgtgcaggg taatttgatg
     2701 gatgagatgc tgattacgat tattcagggg cacgctcagc gactcgggat gaaaaccatc
     2761 gccgggccag tcgttttacc cttagtgatg gatacgcttt ctggcatcgg cgtcgatctg
     2821 atttatggtg atgtgattgc cgatgcccaa ccgctggatt tgctggtgaa tagcagttat
     2881 ttcgcgatta actgagggag cgtttgccca ttgcctgatg cgacgtatca ggcctacaat
     2941 tcgtccgttc cgtctggttg ccagccttcc gtataccaga tatgcaacag cgcataagaa
     3001 cgccagggct tccagcgctc ggcataacgg cggatttgtg ccggtgtcat ccccggaaat
     3061 cgctgtttaa tcagataatc atccggcaga aaaacatctt tcgcctgcca gccacgcaaa
     3121 gcgaaataat tcgccgtcca gcgcccgata cccggaaaag tttgcagcgt tttcatcgcc
     3181 tgctccacat cgcccggtat tgtcattggt aaggtgccct ccagcgccgc atttgccaga
     3241 tgaatcagcg cctctgcccg tttcaacggc atacctaacg cttttaatgc ctgcgggtcg
     3301 gctgctgcca gccgctgagg cgtcgggaag cagatatact ccggaaaatc atccagccgt
     3361 tcgccataaa gctgtaccac tttggcagtc aattttgccg ccatcgccac gctcaccagt
     3421 tggcctaaaa tcgcccgcac accctgctca aaagcatcaa cacagccggg taaacgcaat
     3481 ccgggccggg ccgcgcctaa cttgcccaac gcaccgttaa caatctgcgg gttacattgc
     3541 agatcaagca ggcggctcat tttcgccaga cactcagcgg caacaggttc taaacctgca
     3601 cttaaattta tgtgcagagt atgacgggcc atatccggaa tagcagtcac cacgccgcga
     3661 tattcgccca ccgccagact acgggcataa taatcgtcag cgaccgtttc cacaccactc
     3721 actgcacggg cggcgagaaa tcccaacatc cacgaccagt catacggcgg ctgccagttc
     3781 agggtataca tcgcatctcc ttattcatcc gctttcagca taaacgttat tcagacgcta
     3841 cgctttgctt tcatattccg gttgtcgcga cggcaacatt tcgctaaagt cacgcccctt
     3901 cttcaccggc atggggatta attcgtgttt attggttttg attacggtac agcaaactgt
     3961 tcagtggcgg tcatgcgtga cggtaaaccg caattactga aaatggaaaa cgacagcacg
     4021 ctgctgcctt caatgctttg cgcgccaacg cgtgaagcag taagtgaatg gctgtaccgc
     4081 catcatgatg ttccggcaga cgacgatgaa acgcaggcgc tgttacgtcg ggcgattcgt
     4141 tataaccgcg aagaagatat cgatgttacg gcgaaaagcg tgcagttcgg tctttcctca
     4201 ctggcacagt acattgatga tccagaagaa gtgtggtttg tgaaatcacc aaaatcgttc
     4261 ctcggtgcca gcggcttaaa accgcagcag gtagcgctgt ttgaggatct ggtctgcgca
     4321 atgatgttgc acattcgcca gcaggcgcag gcgcagctgc cagaagcgat tactcaggcg
     4381 gtgattggtc gcccgatcaa cttccagggg ctgggcggcg atgaagcaaa cgcccaggcg
     4441 caagggattc tggaacgcgc ggcgaagcgt gccggattca gggacgtggt attccagtac
     4501 gagccggtcg cggctgggct ggattacgaa gccaccttgc aggaagaaaa acgggtgctg
     4561 gtggtggata tcggcggtgg tacgactgac tgttcattgc tgctgatggg gccgcagtgg
     4621 cgttcgcgtc tcgatcgtga agccagcctg ctgggtcaca gtggttgccg tattggtggt
     4681 aacgatctgg acatcgcact ggcgtttaaa aacctgatgc cgttactggg catgggtggc
     4741 gaaaccgaaa aaggcatcgc cctgccgatt ctgccgtggt ggaatgcggt tgccatcaac
     4801 gacgtacctg cgcagagtga tttctacagt agtgccaacg gtcgtctgct taacgatctg
     4861 gtacgcgatg cccgcgaacc ggaaaaagtg gccctgctac agaaagtctg gcgtcagcgt
     4921 ttaagctatc gcctggtgcg tagcgcagaa gagtgcaaaa ttgctctttc aagcgtagcg
     4981 gaaacccgcg cctcactgcc gtttatcagc gatgaactgg ctacgctgat tagccagcaa
     5041 gggctggaaa gcgccctcag ccagccactg gcgcggattc tggaacaggt gcaactggcc
     5101 ctggataacg cccaggaaaa accggacgtt atctacctga ccggcggtag cgcccgttcg
     5161 ccgctgatta aaaaagcgct ggcagaacag ttgccgggaa ttccgattgc aggcggcgat
     5221 gactttggct ccgtcaccgc cgggctggca cgctgggcgg aagtggtgtt tcgttaaaag
     5281 aaaatggctc agcagcggct taaatctgcc tgcgcctgag ccagtttttc tgccgcctga
     5341 cgtaaaggtt ccatcaacgg catggtccgt gctggagcat gaaggcggaa tcgctgcaac
     5401 tctgtcgcgc cgacagtcaa cgtcgattcc agccgatgtc gtttggcggt cattttcgcc
     5461 attaccgctt gtctgtctgc tggcgttatc gcttcattcg gcctgaacac aaaacggcgt
     5521 tcacagctcg ctttccagtc aataaccgcc tgggtcagat ggtcgccaaa ccctttcact
     5581 tgcttaacgc cacgacgggt aacatctgcg gctgtttcaa taccaaaaga ccgtaacgcc
     5641 gctttacgcg cagggccaac gccgggaata gaggcaacat caataaaaaa tccctccaga
     5701 aacttctgct tctgccgttc ccttgcagtg tcatgaagtg ctgccagagc gcgtttctct
     5761 tcttccggta acccgagaat ttcgtccttc attttttcga gcatcgcccg tttggcgata
     5821 aaaccttcca gcccgcccaa ctgttggatc tggctgacta aatgctcata gtccattttc
     5881 gcgcgattaa atgcctcacg gcgttgctgg atttctgctt tgtacgcttt gcttgtcaga
     5941 ctgccaataa tccagatagc cgacagcaca ggaaccaaaa taatataacg cggttctgcc
     6001 tgaaggccgc aaagcaacag cgataacgct gacagtgcga tctcaatcag aatgatgtat
     6061 tcgcgccgta acaggcctaa aggaagcggc ctgccagtcg gttggaaatg atcgggtaat
     6121 ggcaattgca atgctggcgg tgctactgac gccatcacca tcgcccagac tttcgccagc
     6181 acaaaatcac cgctggtggt aatgacctct tcgccgagat caacaaaata gataacgcct
     6241 tgattatcca gcgcacacca cgggcagtca gccaaatgac cgggataaac atgcattgcc
     6301 gatacggtac atttctttaa ctgttggcgt agagaatcca gtgctgatac ccacgcttta
     6361 gccgtcggac gcccggttgc cacgccactt tccgtaaacg cctgctgaaa catggcttca
     6421 acatcactcg gtaacatcga tagcggaatc gatcgtggcg gcggttttaa accacgtcgc
     6481 tgattatctg acgcgtaggc ataacggaaa tgggtaatat ccgtctccag cgcattaccc
     6541 gcatcagaga taagcggcac accagaataa ggatgccggc caccaaacaa gacgtgaaaa
     6601 atcagcaacg caaggccaaa attatcgtga ttttctgtgc gttcaaagcc gacaaatgac
     6661 gacaaggttt gtagctctgg cggcgtaaaa tgcgacacgc cgacttcgca taaatgcagt
     6721 gtgccattgg cgttaatctg aaaggagtcg ctatcaatca acaccacttt gctgtcgcga
     6781 cctaccataa aactgttctg gtttacgtca cccacgacgt gcccgtgctc gtgaaccgta
     6841 gcaaaagatg aagcaatatt gcgcgcaaca tagagtagaa aatcccacgc acaatgaggg
     6901 taacgttgac gacgatgtgc cgggctatag atcatatgaa tcggttcttt accagaaact
     6961 tttggcatca taaaaccgat aacttttccg cctcgtccac cgtgaagcgt tgcctgcggc
     7021 caggcgacat aattcaacaa ttgcgcgtca gctgtcgcag ccataaaggc aagtttgtcc
     7081 tgttttaagg cgggtggcgg cgtgtgataa atcttggcga cgctatcgac aaactcattg
     7141 atatcataaa ccgcgccttc accgcctttg cccagttcac ggcctggcgt aacgcactca
     7201 ccagtagcag tatataaagt gggtttcatg gtaaccacag cgccagtgcc aacgttttat
     7261 catcatccgt acgctcgttt accgccgggc tggacaaaaa ctgctttaac aattcaggta
     7321 ataaatcgag ttgttcctgc gttgctgacg ccagtccatt gaaaaacggg gtaaagaaag
     7381 gcacatgagg agaattatcc agcatattta acgccagtcg ctggatacca tccgtaaatg
     7441 ctgcaacttt atgcacacgc tcagtgctgg tgaaagtttc cagtctggaa acggcatctt
     7501 catcggtaat gaagtgcgtc atattggcat attcacccac catcggcgta agcggcaatt
     7561 gcagaccgtg accaaaatcc accaccacgc cgccatcgcc aatctgcata atcaaggtgc
     7621 cattagccga tgaaatcaat cccagaaacg tgcaggcaaa atcgcgaact gctaattctt
     7681 tggcttccgc ctcggcaaaa agtcgctgac gaacggtcag cacaatatcc gtggcgagga
     7741 tatcattaag ccccaattcc ccaccctgca ctttttgcga catataagcc atcgcttcat
     7801 tgacggcgag cgtcgcgcct tctcctccct gcgaaacgct acctgcgcca tcagcaagga
     7861 acatcaccag taatggctgc tggtcattta gccaggcaac ctgcatctgg caggcatcct
     7921 ggcaaggcaa gtccgcgctg atgtgcgatg ttccaacagc ggatgcataa accagacgcc
     7981 agctcacact gacgtccagc cttttggcgc ttccagcaca acttccgttc ccggcgtgga
     8041 gcgggaaacc gaacgcagcg aactggataa ccagctaaac agttcacgga attgtaatcc
     8101 ttgcagaggc aaaggctgac gaacgctgat ttgtgccaga gtcttcatat ccgccccctg
     8161 aacaccgatg gaaaagaaag caaacctttt atcttcttcc ccccgaaaca ctttgttggc
     8221 tgcagcctgc cactcatcgg ttggtgcacc atcggtaatc aggaaaatcc acgggcgata
     8281 atacgaaata ccatttgcac gatactcacg tttccgctcc tctaccatat ccagggcttt
     8341 ggtaatggcc gcacccattg gtgtatcgcc ctgggcaaac aggatgggcg gaaaaaaatt
     8401 agcggcgctg gtaaagggtt gttccacatg caccgggccg aacgtcacaa tgccgagttc
     8461 cactcttttt aaagccagcg aatcggcaag cagctcatcg cgaaaggtaa ccaatccggt
     8521 gttaagttca ttaataggtc tgccactcat cgagccagaa acatccagca gcagaatgca
     8581 ggggcaacgt ggttccgggt tactggcaaa atcgctggtg gcaaacgtga tttgttcgct
     8641 cataagaaat cctttcttag agaattattg aggtccggta tcataccgac tgcaataatg
     8701 aaataagagc aaacgaagaa actttcagaa ttaggcccgg cggcgcagaa aattttgcta
     8761 ttcgcctcgc aagaacggag agccgtcgat tgacactaag ggcggagtga cataatttca
     8821 ggagtgaggg ttagggagag gtttccccct ccccctggtg ttcttagtaa gcctggaagc
     8881 taatcactaa gagtatcacc agtatgatga cgtgcttcat cataaccctt tccttattaa
     8941 aagccctctt ctccgggaga ggctttcccg tttcagcgtc ccgctgaaat catcggctta
     9001 cctcctttcg ccctgaatgc agtctatcgc taacgcgtga ataaaatagt ttcctctgtg
     9061 attattgcgt aacgactcgc aaagaatatt tgctggcagg atcgcagact acaaagcctg
     9121 cggattgaca atcttatcgt gaaggcatac tttcaggagt gagggtagag cggggtttcc
     9181 cccgccctgg tagtcttagt aagcggggaa gcttatgact aagagcacca cgatgatgag
     9241 tagcttcatc atgacccttt ccttatttaa ggccccttcc tcgggagggg ctttcccgtt
     9301 tcagcatccg gctgaaatct gcggcttacc tcctttcgcc ctgtatgcag tctatcgcta
     9361 acgcgtagat aaaatagttt cctgtgttat tactggatgc gtgctcgcaa atgtgcccgt
     9421 cattcagacg attccagaca gtgtttcata attcctccat ttttctccct tattggctgg
     9481 ctacactagt atcattccgc gaaacgtttc aggaagagaa actcttaacg atgaaaggta
     9541 gttataaatc ccgttgggta atcgtaatcg tggtggttat cgccgccatc gccgcattct
     9601 ggttctggca aggccgcaat gactcccgga gtgcagcccc aggggcgacg aaacaagcgc
     9661 agcaatcgcc agcgggtggt cgacgtggta tgcgttccgg cccattagcc ccggttcagg
     9721 cggcgaccgc cgtagaacag gcagttccgc gttacctcac cgggcttggc accattaccg
     9781 ccgctaatac cgttacggtg cgcagccgcg tggacggcca actgatagcg ttacatttcc
     9841 aggaaggcca gcaggtcaaa gcaggcgatt tactggcaga aattgacccc agccagttca
     9901 aagttgcatt agcacaagcc cagggccaac tggcaaaaga taaagccacg cttgccaacg
     9961 cccgccgtga cctggcgcgt tatcaacaac tggcaaaaac caatctcgtt tcccgccagg
    10021 aactggatgc ccaacaggcg ctggtcagtg aaaccgaagg caccattaag gctgatgaag
    10081 caagcgttgc cagcgcgcag ctgcaactcg actggagccg gattaccgca ccagtcgatg
    10141 gtcgcgttgg tctcaagcag gttgatgttg gtaaccaaat ctccagtggt gataccaccg
    10201 ggatcgtggt gatcacccag acgcatccta tcgatttagt ctttaccctg ccggaaagcg
    10261 atatcgctac cgtagtgcag gcgcagaaag ccggaaaacc gctggtggta gaagcctggg
    10321 atcgcaccaa ctcgaagaaa ttaagtgaag gcacgctgtt aagtctagat aaccaaatcg
    10381 atgccactac cggtacgatt aaagtgaaag cacgctttaa taatcaggat gatgcgctgt
    10441 ttcccaatca gtttgttaac gcgcgcatgt tagtcgacac cgaacaaaac gccgtagtga
    10501 tcccaacagc cgccctgcaa atgggcaatg aaggccattt tgtctgggtg ctgaatagcg
    10561 aaaacaaggt cagcaaacat ctggtgacgc cgggcattca ggacagtcag aaagtggtga
    10621 tccgtgcagg tatttctgcg ggcgatcgcg tggtgacaga cggcattgat cgcctgaccg
    10681 aaggggcgaa agtggaagtg gtggaagccc agagcgccac cactccagaa gagaaagcca
    10741 ccagccgcga atacgcgaaa aaaggagcac gctcctgatg caggtgttac ccccgagcag
    10801 cacaggcggc ccgtcgcgcc tgtttattat gcgtcctgtc gccaccacgc tgctgatggt
    10861 ggcgatctta ctcgccggga ttatcggtta tcgcgccctg cccgtttcgg cgctgccgga
    10921 agtggactat ccgaccattc aggtggtcac gctctaccca ggtgccagcc cggatgtcat
    10981 gacctctgcc gttaccgcgc cgctcgaacg ccagttcggg cagatgtctg gcctgaaaca
    11041 gatgtcgtcg caaagttccg gcggtgcgtc agttatcact ttgcagttcc agctaacatt
    11101 accgctcgat gtcgccgagc aggaagtgca ggccgcgatt aacgctgcga ccaacttgtt
    11161 gccgagcgat ctgcctaacc cgccggttta cagcaaagtg aacccggcag atccgccgat
    11221 catgacgctc gccgtcacct caaccgccat gccgatgacg caagtggaag atatggtgga
    11281 aacccgcgtc gcgcagaaaa tctcgcagat ttccggcgtc ggcctggtga cgctttccgg
    11341 cggtcagcgt ccggctgttc gcgtcaaact taacgctcag gcgattgccg ccctcggcct
    11401 gaccagcgaa accgtgcgca ccgccattac cggcgctaac gttaactcgg caaaaggtag
    11461 cctcgacggc ccttcccgtg cggtcacgct ttccgcgaac gaccagatgc aatccgccga
    11521 agagtatcgc cagctaatca tcgcctacca gaacggcgcg ccaattcgtc tgggcgatgt
    11581 cgcaactgta gagcaaggtg cagaaaacag ctggctcggc gcgtgggcga acaaagaaca
    11641 ggccattgtg atgaatgttc agcgccagcc cggtgctaac attatctcca ccgccgacag
    11701 cattcggcag atgctgccac agctcactga gagtctgccg aaatcggtga aggtgacagt
    11761 gctttccgat cgcaccacca atatccgcgc atccgtcgat gatactcagt ttgaattgat
    11821 gatggctatc gcgctggtag tcatgattat ctacctgttt ttgcgcaata ttccggcgac
    11881 catcattccc ggtgttgctg taccgctgtc gttaatcggc actttcgcgg ttatggtgtt
    11941 tctcgatttt tcaatcaata acctgacact gatggcgtta actatcgcca ccggattcgt
    12001 ggtcgatgac gccatcgtgg tgatcgaaaa catttcccgc tatatcgaaa aaggcgaaaa
    12061 accgttggcg gcggcgctca agggcgcagg tgaaatcggc tttaccatta tctcgctgac
    12121 cttctcactg attgcggtgt tgatcccact gctgtttatg ggcgatatcg tcgggcgact
    12181 gttccgcgaa tttgctatta cgctggcggt agcgattttg atctcagcgg tggtgtcgct
    12241 gaccctgaca ccgatgatgt gcgcgcggat gctcagccag gagtcgttgc gtaaacagaa
    12301 ccgcttctcc cgtgcctcgg aaaaaatgtt cgacaggata atcgccgcct atggtcgtgg
    12361 actggcgaag gtgctgaatc atccgtggct gaccttaagc gtggcgctca gcacgctgct
    12421 gcttagcgtt ctcttatggg tgttcattcc gaaaggtttc ttcccggtac aggacaacgg
    12481 cattattcag ggcactttgc aggcaccgca atccagctcc tttgccaata tggcccagcg
    12541 acaacgccag gtcgcggacg tgattttgca ggatccggca gtgcaaagcc tgacctcatt
    12601 tgttggcgtt gatggcacta acccgtcgct gaatagtgca cgtttacaaa tcaacctcaa
    12661 accgctggat gaacgtgatg accgagtgca aaaagtcatt gcccgtctgc aaacggcggt
    12721 ggataaagtg ccgggcgtcg atctcttcct gcaaccaacg caggatctga ctattgatac
    12781 tcaggtcagc cgcacccagt accagtttac cttgcaggcc acctcactgg atgcgctcag
    12841 tacctgggtg ccacagttga tggaaaaact ccagcaactg ccacagcttt ctgatgtctc
    12901 cagcgactgg caggacaaag ggctggtggc gtatgtcaat gttgatcgcg acagcgccag
    12961 ccgtctgggg atcagcatgg cggatgtcga taacgccctg tacaacgcgt ttggtcagcg
    13021 actgatttcc actatttata ctcaggctaa ccagtatcgc gtagtgctgg agcacaacac
    13081 cgaaattacc ccgggcctcg cggcgctgga taccattcgc ctgaccagta gcgacggcgg
    13141 cgtggtgccg ttaagctcaa ttgccaaagt tgaacagcgt tttgcgccgc tttccatcaa
    13201 ccatctggat cagttcccgg taacgaccat ctcctttaac gtgccggata actattcgct
    13261 gggcgatgcg gtgcaggcga ttatggacac cgaaaagacg ctgaatctgc cggtggatat
    13321 caccacgcaa tttcagggca gcaccctcgc cttccagtcg gcgctgggca gcactgtctg
    13381 gctgattgtc gcggcggtgg tggcgatgta tatcgtgctc ggcattctgt acgagagctt
    13441 tattcacccg atcaccattc tctcgacgct acccaccgca ggggtcggcg cgctgctggc
    13501 attaatgatt gccggtagcg aactggatgt gattgcgatt atcggcatta ttttgctgat
    13561 cggtattgtg aagaagaacg ccatcatgat gatcgacttc acgctggctg ctgagcgcga
    13621 gcaaggcatg tcgccgcgcg atgcaatcta ccaggcttgt ctgttgcgtt ttcgtccgat
    13681 cctgatgacc accctggcgg ctctgcttgg cgcgctgccg ctgatgttga gtaccggggt
    13741 cggcgcggaa ctgcgtcgtc cgttaggtat cggcatggtc ggcggtctga ttgtcagcca
    13801 ggtgctgacg ctgttcacca cgccggtgat ttatctgctg ttcgaccgcc tggcattgtg
    13861 gaccaaaagt cgctttgctc gccatgaaga ggaggcgtaa gtgaagtttt ttgccctctt
    13921 catttaccgc ccggtggcga cgattttact gtcggtcgcc attaccctgt gcggcatact
    13981 gggcttccgt atgctgccgg tcgccccgct gccgcaggtc gattttccgg tgattatggt
    14041 cagcgcctcg ctgcccggtg cgtcaccaga aacaatggcg tcttccgttg ccacgccgct
    14101 ggagcgctca cttgggcgca ttgccggagt cagtgaaatg acctccagca gttcgctcgg
    14161 cagcacgcgt attattttgc agtttgattt tgaccgggat atcaacggcg cggcgcgcga
    14221 tgtgcaggcg gcgatcaacg ctgcacaaag tttgctgccc agcgggatgc ccagccgtcc
    14281 gacctatcgc aaagctaacc cgtcggatgc gccaattatg atcctcacgc tgacatccga
    14341 tacttattcg cagggtgaac tgtacgattt tgcctcgacg cagctggctc cgacgatttc
    14401 gcaaatcgac ggtgtcggtg atgtcgatgt tggcggcagc tcgctgcccg ccgtacgcgt
    14461 cgggctgaat ccgcaggcgc tgtttaatca gggcgtctcg ctggacgacg tacgcaccgc
    14521 catcagcaat gccaacgtgc gtaaaccgca gggggcgctg gaagatggca ctcaccgctg
    14581 gcagatccag accaatgatg agctaaaaac cgccgccgaa tatcagccgt tgattattca
    14641 ttacaacaac ggcggcgcgg ttcgtctggg cgatgtggcg acggtgaccg actcggtgca
    14701 ggatgtgcgc aacgccggga tgaccaacgc caaaccggct attttgctga tgatccgcaa
    14761 gctgccggaa gccaatatta tccagacggt agacagcatc cgggcaaaat taccggagtt
    14821 gcaggaaacc attccggcgg cgattgatct gcaaattgct caggatcgct cccccaccat
    14881 tcgcgcctcg ctggaagaag tcgagcaaac gctgattatc tcggtggcgc tggtgattct
    14941 ggtggtgttt ttattcctgc gctcgggtcg cgccactatt attcccgccg ttgcggtgcc
    15001 ggtttcgctg attggtacgt ttgcggcgat gtacctgtgc ggattcagtc tcaataacct
    15061 ttcgttaatg gcgctcacca tcgctactgg tttcgtggtg gatgacgcca tcgtggtgct
    15121 ggaaaacatt gcccgtcatc tggaagcggg gatgaaaccg ctacaggccg cgctacaagg
    15181 tactcgcgaa gtcggcttta cggtgctgtc gatgagtctg tcactggtgg cggtgttcct
    15241 gccgctgctg ttgatgggcg gattgccggg ccgactgtta cgcgaatttg ccgtgacgct
    15301 ttctgtcgcc attgttatat cgttgctggt ttctctgaca ttaacgccaa tgatgtgtgg
    15361 ctggatgctg aaagccagca agccgcgtga gcaaaagcga ctgcgtggtt ttggccgcat
    15421 gttggtagcc ctgcaacaag gctacggcaa gtcgctgaaa tgggtgctca atcatacccg
    15481 tctggtgggt gtggtgctgc ttggcaccat tgcgctgaat atctggctgt atatctcgat
    15541 cccgaaaacc ttcttcccgg agcaggacac cggcgtgctg atgggcggga tacaggcgga
    15601 tcagagcatt tcgtttcagg cgatgcgcgg caaattgcag gatttcatga aaattatccg
    15661 tgacgatccg gcagtggata atgtcaccgg ctttaccggc ggttcacgag ttaacagtgg
    15721 gatgatgttt atcaccctca agccacgcga cgaacgcagc gaaacggcgc agcaaattat
    15781 cgaccgtctg cgcgtaaaac tggcgaaaga accgggggcg aatctgttcc tgatggcggt
    15841 acaggatatt cgcgttggtg ggcgtcagtc gaacgccagc taccagtaca cgttgttatc
    15901 cgacgacctg gcggcactgc gcgaatggga gccgaaaatc cgcaaaaaac tggcgacgtt
    15961 gccggaactg gcggacgtga actccgatca gcaggataac ggcgcggaga tgaatctggt
    16021 ttacgaccgt gacaccatgg cacggctggg aatcgacgta caagccgcca acagtctgtt
    16081 aaataacgcc ttcggtcagc ggcaaatctc gaccatttac cagccgatga accagtataa
    16141 agtggtgatg gaagtggatc cgcgctatac ccaggacatc agtgcgctgg aaaaaatgtt
    16201 cgttatcaat aacgaaggca aagcgatccc gctgtcgtat ttcgctaaat ggcaaccggc
    16261 gaatgcccca ctatcggtga atcatcaggg attatcggcg gcctcgacca tttcgttcaa
    16321 cctgccgacc ggaaaatcgc tctcggacgc cagtgcggcg atcgatcgcg caatgaccca
    16381 gcttggtgtg ccttcgacgg tgcgcggcag ttttgccgga acagcgcagg tgttccagga
    16441 gacgatgaac tcacaggtga tcctgattat cgccgccatc gccacggtgt atatcgtgct
    16501 gggtatcctt tacgagagtt atgtgcaccc gctgacgatt ctctccaccc tgccctcggc
    16561 gggtgttgga gcgctgctgg cgctggagct gttcaatgcc ccgttcagcc taatcgccct
    16621 gatagggatc atgctattaa tcggcatcgt gaagaaaaac gccattatga tggtcgattt
    16681 tgcgcttgaa gcccaacggc acggtaacct gacgccgcag gaagctattt tccaggcctg
    16741 tctgctgcgt tttcgcccga ttatgatgac taccctggcg gcgctgtttg gcgcgctgcc
    16801 gctggtgttg tcgggcggcg acggctcgga gctgcggcaa cccctgggga tcaccattgt
    16861 cggcggactg gtaatgagcc agctccttac gctgtatacc acgccggtgg tgtatctctt
    16921 tttcgaccgt ctgcggctgc gtttttcgcg taaacctaaa caaacggtaa ccgagtaaat
    16981 gacagatctt cccgacagca cccgttggca attgtggatt gtggctttcg gcttctttat
    17041 gcagtcgctg gacaccacca tcgtaaacac cgcccttccc tcaatggcgc aaagcctcgg
    17101 ggaaagtccg ttgcatatgc acatggtcat tgtctcttat gtgctgaccg tggcggtgat
    17161 gctgcccgcc agcggctggc tggcggacaa agtcggcgtg cgcaatattt tctttaccgc
    17221 catcgtgctg tttactctcg gttcactgtt ttgcgcgctt tccggcacgc tgaacgaact
    17281 gttgctggca cgcgcgttac agggcgttgg cggcgcgatg atggtgccgg tcggcagatt
    17341 gacggtgatg aaaatcgtac cgcgcgagca atatatggcg gcgatgacct ttgtcacgtt
    17401 acccggtcag gtcggtccgc tgctcggtcc ggcgctcggc ggtctgctgg tggagtacgc
    17461 gtcgtggcac tggatctttt tgatcaacat tccggtgggg attatcggtg cgatcgccac
    17521 attgatgtta atgccgaact acaccatgca gacgcggcgc tttgatcttt ccggcttttt
    17581 attgctggcg gttggcatgg cggtattaac cctggcgctg gacggcagta aaggtacagg
    17641 tttatcgccg ctggcgattg ctggcctggt cgcagttggc gtggtggcac tggtgcttta
    17701 tctgctgcac gccagaaata acaaccgcgc cctgttcagt ctgaaactgt tccgcactcg
    17761 taccttttcg ctgggcctgg cggggagctt tgccggacgt attggcagtg gcatgttgcc
    17821 ctttatgaca ccggttttcc tgcaaattgg cctcggtttc tcgccgtttc atgccggact
    17881 gatgatgatc ccgatggtgc ttggcagcat gggaatgaag cgaattgtgg ttcaggtggt
    17941 gaatcgcttt ggttatcgtc gggtactggt agcgaccacg ctgggtctgt cgctggtcac
    18001 cctgttgttt atgactaccg ctctgttggg ctggtactac gttttgccgt tcgtcctgtt
    18061 tttacaagga atggtcaact caacgcgttt ctcctccatg aacaccctga cgctgaaaga
    18121 tctcccggac aatctggcga gcagcggaaa cagcctgctg tcgatgatta tgcaattgtc
    18181 gatgagtatc ggcgtcacca tcgccgggct gttgctggga ctttttggtt cgcagcatgt
    18241 cagcgtcgac agcggcacca cacaaaccgt ctttatgtac acctggctta gcatggcgtt
    18301 gatcatcgcc cttccggcgt tcatctttgc cagagtgccg aacgatacgc atcaaaatgt
    18361 agctatttcg cggcgaaaaa ggagcgcgca atgaagttct ggcgacccgg tattaccggc
    18421 aaactgtttc tggcgatttt cgccacctgc attgtcttgc tgatcagtat gcactgggcg
    18481 gtgcgtatca gttttgagcg tggctttatt gattacatca agcatggtaa tgaacaacga
    18541 ttacaactgt taagtgatgc gcttggcgag cagtatgcgc agcatggcaa ctggcgcttc
    18601 ctgcgcaaca atgatcgctt tgtctttcag atcctgcgtt catttgaaca cgataattcg
    18661 gaagataaac ccggcccggg tatgccaccg cacggctggc gtacccagtt ctgggtggtt
    18721 gatcaaaaca acaaagtgct ggttggtccg cgagcgccga ttccacctga cggtacacgg
    18781 cgacccattc tggtcaacgg tgcggaagtt ggcgcggtga tcgcctcccc cgttgagcgg
    18841 ttaacgcgca atactgatat caatttcgat aaacaacagc ggcaaaccag ctggctgatc
    18901 gtcgccctgg caacgttact cgcggcgctc gccacctttc tgctggcgcg cggtttgctg
    18961 gcaccggtaa aacgacttgt cgatggcacg cacaaactgg cggcgggcga tttcactacc
    19021 cgcgtaacgc ccaccagtga agatgaactg ggcaaactgg cgcaagactt caaccagctt
    19081 gccagcacgc tggagaaaaa ccagcaaatg cggcgcgatt ttatggccga tatttcacac
    19141 gaactgcgta cgccattagc ggtgctgcgc ggtgaactgg aagccattca agatggcgtg
    19201 cgcaaattca cgccggagac ggtggcgtct ttacaggcgg aagtcggtac actgactaaa
    19261 ctggttgacg atcttcatca gttgtcgatg tctgatgaag gcgctctcgc ctatcaaaaa
    19321 gcaccggtag atttgatccc actgctggaa gtggcgggcg gcgcatttcg cgaacgattt
    19381 gccagccgtg gcctgaaact gcagttttcc ctgccagaca gtattaccgt atttggcgat
    19441 cgcgaccgtt taatgcaatt attcaataac ttactcgaaa acagcctgcg ctacactgat
    19501 agcggcggca gcctgaaaat ctctgccgag cagcacgaca aaacggtgcg cctgaccttt
    19561 gccgacagtg cgccaggtgt cagtgacgag cagctacaaa aattatttga acgtttttat
    19621 cgcaccgaag gttcccgcaa ccgcgccagc ggtggttccg ggctggggct ggcgatttgc
    19681 ctgaacattg ttgaagcaca taatggccgc attattgctg cccattcgcc ttttggcggg
    19741 gtaagcatta cagtagagtt accgctggaa cgggatttac agagagaagt atgaccgagt
    19801 taccaatcga cgaaaacaca ccgcgtattt tgatcgtgga agatgaaccg aagctggggc
    19861 agttgctcat tgattatctg cgtgctgcga gctatgcgcc gacgcttatc agccacggcg
    19921 atcaggtact gccgtatgtg cgccagacac caccggatct gatcctgttg gatctgatgc
    19981 tccctggcac cgatggcttg acgctgtgcc gggaaattcg tcgtttttct gacattccta
    20041 tcgtgatggt gacggcaaaa atcgaagaga tcgaccgcct gctcggactg gagattggcg
    20101 cagatgacta catctgcaag ccgtacagcc cacgggaagt ggtagcgcgc gtcaaaacca
    20161 ttttgcgccg ttgcaaacca cagcgcgaat tgcagcaaca ggatgccgaa agcccgttga
    20221 ttatcgacga aggtcgtttt caggcttcat ggcgcggtaa aatgcttgac ctgacacctg
    20281 cggaatttcg tctgttgaaa acgctctctc atgaaccagg aaaagtgttc tcccgcgagc
    20341 aattgctcaa tcatctttat gacgactacc gcgtagtaac cgaccgcacc attgacagcc
    20401 acattaaaaa cctgcgccgc aagctggaat ctctcgacgc cgaacagtca tttatccgcg
    20461 ccgtttacgg cgttggttac cgctgggaag ccgacgcctg ccgcatcgtt tagttttagc
    20521 gacatgactt tgttagccgg agatggcgtt cagaccagat ccggcaacat tatcccacgc
    20581 atggtcagca aactgacatt atctctccgc cctggcaggc atcagaaaat cgctcatact
    20641 ttaatcggta aacagcacct ttagatgctg ttttcgatac gcaatttcaa tcaaggagtc
    20701 attatggctg gttggtttga actcagtaag agcagtgata atcagttccg gtttgtgcta
    20761 aaagcgggca atggtgagac tatcctcacc agcgagctgt atacctcaaa agcctctgcg
    20821 gaaaagggca tcgcgtcggt gcgtagcaac agcccgcaag aagaacgcta tgagaaaaaa
    20881 acggcaagta acggcaaatt ctatttcaat ctgaaagccg ctaatcatca aattatcggc
    20941 tccagccaga tgtacgccac cgcgcaatct cgtgaaaccg gaattgcctc cgttaaagcc
    21001 aatggcacaa gccagacggt gaaagacaat acgtaattac gatgccgggc gtgggttgtt
    21061 ggcgtccggc tttatatcca aataacgctt agttattaat aattaagagt aaccgccgcc
    21121 attaaaatag tgggcactga cgagcactcg ttgtgctttc tttgtttacc atcccatact
    21181 tatcctcctg aacatgttca tgtgccaaaa tatcttctaa tactttcgtg acgctatccg
    21241 gtttatgaca tacactgatg taagattttt acttacaggc gggcgtaaaa tggccagaac
    21301 gatgacggtt gatctcggcg atgagttgcg tgaatttatc gagtccttaa ttgaatccgg
    21361 cgactatcgc acccaaagcg aagtgatccg tgagtcactg cgattacttc gagaaaagca
    21421 agcagagtct cgcctgcaag cgttacgtga catgctcgca gaagggttga gtagtggtga
    21481 agctcagccg tgggaaaagg acgcgttctt acggaaggtg aaagcaggga taaggaaata
    21541 atgtgcgtat tattaaactc atgccaaaag ccaatgagga tttagaaggc atctggtatt
    21601 acagctatca ccatttcggc gaaccacaag ccgacagata tgtggaacat ctttcagacg
    21661 ttctccagat tctgagtaac aataatatag gtacgcccag acctgaatta ggcgaaggta
    21721 tttttgttct tccttttgaa cgccacgtca tttatttcct gcaatcacca ggggaaatta
    21781 tcgtcattcg tatccttaac caaaaccagg acgccaccag acatttacac tggagctaaa
    21841 cccgccgatt gcgctacaat gcccgccctt aaagtggggg cactccccta accgcttcat
    21901 caggtgaagc ggatctgacc tgtcatcaga acgagagaat tatgtttaaa ccggaactcc
    21961 tttccccggc gggaacgctg aaaaatatgc gttacgcttt cgcttatggc gcagatgctg
    22021 tttatgcggg ccagccgcgt tattccctgc gtgtgcgcaa caacgaattc aaccacgaaa
    22081 atcttcagct cggcatcaat gaagcccacg cgctggggaa aaagttttat gtcgtggtca
    22141 acattgcacc gcacaacgcc aagctgaaaa cctttatccg tgacctgaaa ccggtggtgg
    22201 aaatggggcc ggatgcgctg attatgtccg atccggggct gattatgctg gtgcgggagc
    22261 acttccctga aatgccgatc catctctcgg tacaggctaa cgccgtgaac tgggcgacgg
    22321 tgaaattctg gcagcaaatg ggactgaccc gcgtgatcct ctctcgcgag ctgtcgctgg
    22381 aagagattga agagatccgc aatcaggtgc cggatatgga ggtagagatc ttcgtgcacg
    22441 gcgcgctgtg catggcctac tccggtcgct gcctgctctc tggctatatc aacaagcgcg
    22501 acccgaacca gggcacctgc accaacgcct gccgctggga gtacaacgtc caggaaggga
    22561 aagaagatga tgttggcaac atcgtacaca agtacgagcc gattccggtg caaaatgttg
    22621 agccgacgct gggtatcggc gcaccaaccg acaaagtgtt tatgatcgaa gaagcccagc
    22681 gtccgggcga gtatatgacc gcgtttgaag atgagcacgg cacttacatc atgaactcga
    22741 aagatctgcg cgctatcgcc catgtagaac gcctgaccaa aatgggcgtg cattcgctga
    22801 aaatcgaagg ccgtaccaaa tctttctact attgcgcacg caccgcacag gtttaccgca
    22861 aagctattga tgacgccgct gcgggcaaac cgtttgacac cagcctgctg gaaaccctgg
    22921 aaggcctggc gcatcgtggc tataccgaag gtttcctgcg tcgtcatact cacgacgatt
    22981 atcagaacta cgaatacggt tattcagttt ctgaccgcca gcagtttgtt ggtgagttta
    23041 ccggtgagcg caagggggac ctcgcggcgg tagcggtgaa aaataaattc tccgttggcg
    23101 acagccttga gctgatgacg ccgcaaggca acattaattt tacccttgag cacatggaaa
    23161 acgccaaagg cgaagctatg ccgatagcac caggcgatgg ttatactgtg tggctcccgg
    23221 tcccgcagga tcttgagctc aattacgcgc tgctgatgcg taatttctcc ggggaaacca
    23281 cgcgtaatcc ccacggtaag tgattaattt cgattatttt tcccggatgg aaaattctta
    23341 gaaaccgatc acatacagct gtatttatta aggttatcat ccgtttcgct gaaaaacata
    23401 acccataaaa tgctagctgt accaggaacc acctccttag cctgtgtaat ctcccttaca
    23461 cgggcttatt tttttatgca taagccctat ctctggtaac cgtcttccat tgaccacatc
    23521 gatagaatcc tccttcatag cacgatgcct ttcacttatc ggcatcgtgc tcccacaggt
    23581 tccggctacg cacagccaga acgcgcatat ttgacgctta ccaaaaaata ttctcactct
    23641 ccacatttga atgtcagacg agcgacgcca tgtaatcctg caccttctgt cttcaggtca
    23701 actatctgca tttttttgcc ctgagtaaca cagaaatggg ctgcatcatt ttttactata
    23761 ttttctgcac cagatattct gctcctggct aaagaagctt cggcttcggt gtagtattgg
    23821 ttatcgagtt tacgctgaat attactttta tatgcaagac caaatttacc gatacttgtc
    23881 tcatcattat gcacagcaca accagacata ataaaaatac taattaatga tatagcagct
    23941 atctttttca tctcaccttc ccccattaaa taccaacgac actctctagt gtttaaatat
    24001 aataatggca tgattattat aattgaatag gattataata aatgttctgt acaacatttc
    24061 ctacataagt aggaattacg gacattgagg cccttcaggg taactccatg ggggctttaa
    24121 tatattatat tgaagatgcc actgtttagt tgaatattag gtatatgctc ttttttgaaa
    24181 tttatcggtg gcagccgtta gtattcgctg tccccattgc aagctcctgg tggtaaccac
    24241 tgaatcctcc atacttgaac tgacttttta tcctccgact ttcatcctgt tctgactcca
    24301 ccttttgttt tctgctctac actatctaca gaccaatcat aaaggcacat acgatcatgg
    24361 cagaatttcc cgccagctta ctgattctta atggcaaaag tactgacaat ctacccttgc
    24421 gcgaagcaat tatgctgttg cgtgaggaag gaatgacgat ccatgtgcgg gtcacctggg
    24481 agaaaggcga tgccgcacga tatgtagagg aggcccggaa gttgggcgtc gcaacggtga
    24541 ttgccggtgg tggcgatggc accattaatg aagtttctac ggcgttgatt cagtgtgagg
    24601 gggatgacat acccgcgctg ggaattttgc cattaggaac cgccaatgat tttgccacca
    24661 gtgtagggat tcctgaggca ctggataagg cgctgaaact ggcaattgcc ggtaacgcca
    24721 ttgcgataga tatggcgcag gtcaacaaac aaacctgttt tattaatatg gcgacaggcg
    24781 gatttgggac gcgtattacc acagaaacgc cggaaaaatt aaaagccgcg ctgggtggcg
    24841 tctcttacat cattcatggc ttaatgcgca tggatactct gcaaccggac cgttgtgaaa
    24901 tccgcggtga aaactttcac tggcaaggtg acgccctggt cattggtatt ggtaacgggc
    24961 gtcaggccgg tggcggtcag caattgtgtc cgaacgcgtt aattaacgat ggcttgctgc
    25021 aactgcgcat ttttaccggc gatgaaattc ttccggctct cgtatcaacc ttaaaatctg
    25081 acgaagataa cccgaatatt atcgaaggcg cttcgtcgtg gtttgatata caagccccac
    25141 acgaaatcac ttttaatctt gatggcgaac cgttgagcgg acaaaacttt catattgaaa
    25201 tacttccggc ggcgttgcgt tgtcgattac caccagattg tccattattg cgttaatcaa
    25261 actattctct cttgaaccag ggagaatccg taatgccgga tgcggtgtaa ataccttatc
    25321 cggcatacag aacaatacta caccgtaatt aatttcaccc gtgtcttctg caatgccagt
    25381 ttatccacct cagacacagc atcatcggta ataataatgt caattttttc gattggcagt
    25441 acctgattaa aaccgcgacg gttaaatttc gatgaatcga gaacggcaat cactttatta
    25501 gcggctgtga ccatcgcacc gctaacggaa taaccctcat taaaggtcgt aataccatta
    25561 accgcatcaa taccatcagc accgacaaac attaaatcgg cattaatatc ctgcaatgaa
    25621 cgctcggcaa tggaaccatg catcgagcgc gttttatggc ggaccgtgcc accacagacg
    25681 accagagtaa tgtctttatt ttcggaaagg gcaaacgctg ccgggagact gttggtaatc
    25741 accgtgatat ttttggcggt cattaatcct tcggcgataa gcattgtggt acttccgctg
    25801 tcgagaataa cggtcatccc ttcatggatc attttgactg ccgcctgcgc tattctgttt
    25861 ttgggcgcgc tggcgagctg aaatcgctct ttaaacccga cttcctgggt ctcggtttca
    25921 ctattaccgg acattatttt cgccgcaccg ccatgaaagc gcgtaaccac gcctttttgt
    25981 tcgagaaagc gcaaatcggc acggattgtc gcttccgagg cagcaaatac tcccgccaga
    26041 tcctgaacaa gcacggttcc ctgttcattc actaattgga tgatcttatt ccttcgctcg
    26101 aatgaattca tcgtcattta cccatattca attgtggcta gtgtaaacga agggggacca
    26161 ttggtgaatg atctgttttg cacaataccg ggccagcatc acgctggccc gcggtttcat
    26221 ggaatgagca acactttgcc cggcatggca ttacgagcga tgtcacgcac cgcctgggtg
    26281 aagctttcaa agcttccacg gtgagcgatt aatggctcca ggcttaactt acgttctgtc
    26341 agcaaccggc tcgccgtttc ccactcctgc cccggccaag ggctggaata gttcatccaa
    26401 ctgccgataa ccgtcagctc tttacgcaat attttgccaa acgttgccga tgttaaatgc
    26461 agatcctgat gcaacgtgcc caccagcgcc agttgggcat gaggaccggc aatctctacc
    26521 gccagttcga cagtttgcgg tacgccagcc gtctcgagga taagctgatt aaaacgcagt
    26581 tcgcgtaaaa cgctctgcat ttgcggcgcg ctcatttcgc tactgttaaa tgtttgcatc
    26641 gcaccgaaag attttgccag tgccagtttt tctgagctaa tgtcgatagc cgtcacactc
    26701 tttgctccca gcgcgacagc gcactgaata gccagcaggc caatggttcc ggcaccaata
    26761 ataataacgt ttttattctc acaaccttgc gctaaatgaa aagcatgcag accaacggta
    26821 atcggctcaa taaaagcccc atcctcaata ggcatatccg tgggtagagc aaagacattt
    26881 tttcgcttaa cgacaatata ttcagcaaat ccaccatcac gccgcgagcc aataaaatca
    26941 tattttgcgc actgggaata aaaccctttc agacactctg gacaagtaaa acagggtaat
    27001 aacggcacac aggcaaccgc atcgccaggg tgtaaatcat caacaccgga tcccaccgca
    27061 tcaatatagc cgctaaattc atggcctaac gttattggat aataatgtgc accattttta
    27121 aatatcctgg gtaaatcgga accacataag cccgagctgg caatttttac ccgcacctca
    27181 tcctgatgtt taatttcagg aatgacgctt tctgcaacgc gcacgatacc atcagtatca
    27241 ttcaccactg atttcataaa aactcctgat tgttaagggg gataacctcc ccctcaggta
    27301 aaaattattc tgcgagaacg actttctctt gtttaataaa gccacgcgct ctacgccagg
    27361 tcatgaaaat accggtcaga taaattgcac cgataataat gaaaccggga atattttgcg
    27421 gggagaaaac ctgaatcagt aaccaggtaa tgggagaacc gccctgatcc attgaagcca
    27481 ccataccccc ggcttttaac gccccagcat tagccgccag ttgggtgtgc aaaccaatag
    27541 tttgcgtcgc gatccacagg gtgatgctca taatgatgac acccgagatt aaggtgcgga
    27601 acagatttcc acgatgcacg gcgaccgcca tcgccacgaa gaagccgatg gtggcaagat
    27661 cgccaaacgg cagcacctga ttacccggca cacaaacagc aattaaaatg gtgagtggga
    27721 taaaaatcag gcttgccgat accaccgccg tatgtcccag cagcaacgcc ggatcaaggc
    27781 caatcaggaa ctcctgaccg ccgaacttcg cctgtaaacg actacgagcc tgcttagcga
    27841 tgggcgttaa accatccatg atgggtttaa tcacccgtgg cattagcagc atcactgccg
    27901 ccgtttttac cgccagctgc aatacacctt tgacatcgta acccgcgagg atgccgataa
    27961 tcagccccat cacaaaaccc acggtgacag gctcgccaaa tggaccaaat ttgcgctgaa
    28021 tatcgtcggc gctaaattta attcggttaa cgcctgggat tttctcgatg atagcatcga
    28081 ccagcaccgc aatcggcccc atatacgccg acgtaccgtg cggaatagca ataccttcca
    28141 gctcaaagaa atttcgggta tcgcgggcaa accagtcgcc gagcttataa acaaacgccg
    28201 cgtgaattac cacacctgcc atccctatca tccatgaacc ggttgccaga tgcagcaacg
    28261 cgccggtgaa ggtcatatgc cagatattcc agatatcaac atttaccacc cgcgtcatac
    28321 gggtcagtaa catcgccacg ttaaccaata tcgcaatcgg aatcgccacc agcgcaattt
    28381 gcgacgccca ggtcattggt gaagagcccg gccagccgac atcgaccaca tgcagattca
    28441 ggtcgaaatt ttccgccatc gctttcgccg ccggaccaat ggaatccagc attaagccaa
    28501 tcacaaggcc aatgccaaca aagccaatcc cgatatgcag acccgcttta aagcaatcgc
    28561 ctgccttcat gcctaatatt ttagaaaaaa taatgatgac aatcggaagc atcaccgtag
    28621 ggccgaggtc gagaatataa cgcatgactt ctgaaaacat aggtcacccc tgtaagatag
    28681 tcagaatttt attttgtaat gcttcgatac cgacaccaga aataaaaggc atgccgtgaa
    28741 ctaacggaat atcgccaaaa ctacgatcca ctttggcagt ggtgcatatc aaatgcacac
    28801 catccatata ggtttctatt tcattaaccc gacactggat taattcaaca ggaatattat
    28861 gattctgaca caactcttta atttcttccg ccgccatcgt agaggtcgca accgcgcctc
    28921 cgcaagcgac aataatcttg cgtttcataa atagtccttt ttattgttac agagggattt
    28981 attcgtctga atctaaaaca tactttgtga agtattcctt taactgggtt tcaggaagag
    29041 tgattagtgt ctcgacgata tcgggctgtt gtaacttgcc aaataaacag cgtaaaagtt
    29101 tcaattgctg ctgcggattt tccacaatca acgcaataac caacgatacc tccacgtcgt
    29161 tatcatcatc cgcttgctga aaatgaactt tatttgttgg ccttaacaga taaatggctg
    29221 acgacttagc atgaatcgcc tcacaatgcg gtattgcaat ggcgtgctgc tcaagcatta
    29281 tcccggtagg gaattctgct tctctggcaa ttaatgcctg tggccaggta tcatgaacca
    29341 cacctttggc gagcatctca ttaccgatat gggttaaaac ttcgctacga tcgacaaaag
    29401 aaattccgct acgaacaaac aggttagtca tataccgtcc ttattccgca cagccgtagc
    29461 gataggcgcg caaaacatca taaattttat ccataatcag ctgatgcggt attgctgata
    29521 attccccgga ctgaatgcgt tcaaattgtt tgggaagata ctgactaatc atgcccagtg
    29581 ggatgtccac gccttcaaga ttcaccatca tcgtttcgac gctattttta atccgactat
    29641 gcggccagta ataacgaata cgatccgaca ggctgtaacg aatatccagt aatgaatcgt
    29701 taaaacccgt acgataatat tttttccagt attgcggttc gtcgagcatc acttcttcaa
    29761 ttaccgccag gcaaccgctg cgattttcgg gggcgataag ttcctgctca atttgtgcca
    29821 gtgcaaatat cgcctcgcgt aaagcaaagg ttaatgcggg accgactttc aatattgcaa
    29881 agtgatcgcg gactaattcc caataagccg tccgggtctg gtaatcggta gaatgtgctt
    29941 cataaaccat tcgggtgttt tctatccatt gcgccagcgc ctgcgcttcc tgcggctgat
    30001 aatggataat attgctgtga tcaaattcca cacccggctg caccacgatg gcaatcacac
    30061 gtgttaacgc ctctgtcagc ccacgggcaa taaaggcctt ttgatgcgta cgtaaagtat
    30121 tggcggcatc ttcaacatgg gtgatgtgta ctgactgaat ggcgctggcc tcaccgcccg
    30181 gaaccggaac ttcggtgcca atgacatagc tcagttgctc acgctggcaa tctgtcgcca
    30241 cactttccgc agcaaagcaa agcacagcag ctcgttccgc aaccgtttct ggtgctaacg
    30301 gtatgggatc ccccgcgcag gacattgacg catcaagatg aattttactg aagccagcac
    30361 gaacatatgc ttttaccagc tcgacggatt tttccatcgc cgcatccgca ttttcttgct
    30421 gccagcagtt tggccccaga tgatcgccgc cgagaataat gcgttcgcgt gcaaacccaa
    30481 ctttatcggc aatcgtaaaa acaaattcgc gaaagtctgc cggtgtcatt ccggtataac
    30541 cgccaaattg attgacctgg tttgacgttg cttcaatcag cactttgcgc gtgctgttgc
    30601 gatcaaatgc cagcgccgct tcgataacca acggatgggc agaacagact gaacatatgc
    30661 cgatatgttc accagcttta tgccgggcaa ttaacgtttt catgtttttt ccttattaaa
    30721 tggcaggtgc gttatgccct gccctcgcag ccacaatcgg caatcacttt gctcaccaca
    30781 tcgcgcattg cggatttagc cgactgcaaa taatcccggg gatcggtcgc ttcagggtgc
    30841 tcggtcaggt aattttttaa cgcctgcgag aaggcatttt tcagctccgt tgcaacgttg
    30901 attttgcata tccccagttt gatggtttgc tgaatatcct tagtcgataa ccctgacgcg
    30961 ccatgcagca ccagcggtaa gttcacccac tggcgaatgt tctccagtct agaaaaatca
    31021 agcgccggtg cgctggcata catcccatga gccgtgccga tggcgaccgc cagggaatca
    31081 attccggttg cctcggcaaa ttcacgcgcc tgagcggggt tggtataaaa agcatcggct
    31141 tcattgactt gcacatcatc ttcctggccg ccaagttgcc ccagctccgc ttcgacgctg
    31201 acatcaaagc gatggcaaaa atccaccacc tctttgaccc gtgaaatatt ttgcgcaaaa
    31261 ggcaaatgcg aggcgtcaat catgactgag cgcacgccag aacgaacctt ctgagcgata
    31321 tcgtcaaatt tcgtgtgatg gtcgagatga attgccagtg gatggtgata ttgcttcgcc
    31381 atcgcgctga ccagcgccaa cagattttct gtaccagcat gagtaaatgt gccaggcgtt
    31441 ccggcgatga tgaccggcgc atgcaggttg gcagcggttt ccaccaccac ttgcatcgtt
    31501 tcgagattgt gaatattgaa tgccggaacc gcataaccgc cgcgctgtgc gttgttcagc
    31561 atctgctttg tcgataccac g
//
LOCUS       DAZKIV010000038        31019 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZKIV010000038 DAZKIV010000000
VERSION     DAZKIV010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.038"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHOT6_001760"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHOT6_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9946992.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            461..844
                     /gene="secE"
                     /locus_tag="ACHOT6_001761"
     CDS             461..844
                     /gene="secE"
                     /locus_tag="ACHOT6_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFP9946993.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            846..1391
                     /gene="nusG"
                     /locus_tag="ACHOT6_001762"
     CDS             846..1391
                     /gene="nusG"
                     /locus_tag="ACHOT6_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFP9946994.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOT6_001763"
     CDS             1550..1978
                     /gene="rplK"
                     /locus_tag="ACHOT6_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFP9946995.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOT6_001764"
     CDS             1982..2686
                     /gene="rplA"
                     /locus_tag="ACHOT6_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFP9946996.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOT6_001765"
     CDS             3099..3596
                     /gene="rplJ"
                     /locus_tag="ACHOT6_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFP9946997.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOT6_001766"
     CDS             3663..4028
                     /gene="rplL"
                     /locus_tag="ACHOT6_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFP9946998.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOT6_001767"
     CDS             4348..8376
                     /gene="rpoB"
                     /locus_tag="ACHOT6_001767"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFP9946999.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOT6_001768"
     CDS             8453..12676
                     /gene="rpoC"
                     /locus_tag="ACHOT6_001768"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFP9947000.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHOT6_001769"
     CDS             12889..13428
                     /gene="yjaZ"
                     /locus_tag="ACHOT6_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HFP9947001.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHOT6_001770"
     CDS             complement(13838..14971)
                     /gene="thiH"
                     /locus_tag="ACHOT6_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFP9947002.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHOT6_001771"
     CDS             complement(14968..15738)
                     /gene="thiG"
                     /locus_tag="ACHOT6_001771"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFP9947003.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMMEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETCAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHOT6_001772"
     CDS             complement(15740..15940)
                     /gene="thiS"
                     /locus_tag="ACHOT6_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFP9947004.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHOT6_001773"
     CDS             complement(15924..16679)
                     /gene="thiF"
                     /locus_tag="ACHOT6_001773"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFP9947005.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQKINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHOT6_001774"
     CDS             complement(16672..17307)
                     /gene="thiE"
                     /locus_tag="ACHOT6_001774"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFP9947006.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGVSLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHOT6_001775"
     CDS             complement(17307..19202)
                     /gene="thiC"
                     /locus_tag="ACHOT6_001775"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFP9947007.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19283..19383)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHOT6_001776"
     CDS             complement(19435..19911)
                     /gene="rsd"
                     /locus_tag="ACHOT6_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFP9947008.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLMLLVLDAARVKHPA"
     gene            20006..20779
                     /gene="nudC"
                     /locus_tag="ACHOT6_001777"
     CDS             20006..20779
                     /gene="nudC"
                     /locus_tag="ACHOT6_001777"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFP9947009.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20819..21883
                     /gene="hemE"
                     /locus_tag="ACHOT6_001778"
     CDS             20819..21883
                     /gene="hemE"
                     /locus_tag="ACHOT6_001778"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFP9947010.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21893..22564
                     /gene="nfi"
                     /locus_tag="ACHOT6_001779"
     CDS             21893..22564
                     /gene="nfi"
                     /locus_tag="ACHOT6_001779"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFP9947011.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHOT6_001780"
     CDS             22607..23197
                     /gene="yjaG"
                     /locus_tag="ACHOT6_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFP9947012.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23384..23656
                     /gene="hupA"
                     /locus_tag="ACHOT6_001781"
     CDS             23384..23656
                     /gene="hupA"
                     /locus_tag="ACHOT6_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFP9947013.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHOT6_001782"
     CDS             23669..24364
                     /gene="yjaH"
                     /locus_tag="ACHOT6_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFP9947014.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SNFCRWQPNEKTF"
     gene            complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHOT6_001783"
     CDS             complement(24366..24791)
                     /gene="zraP"
                     /locus_tag="ACHOT6_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFP9947015.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25029..26405
                     /gene="zraS"
                     /locus_tag="ACHOT6_001784"
     CDS             25029..26405
                     /gene="zraS"
                     /locus_tag="ACHOT6_001784"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFP9947016.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKERLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26402..27727
                     /gene="zraR"
                     /locus_tag="ACHOT6_001785"
     CDS             26402..27727
                     /gene="zraR"
                     /locus_tag="ACHOT6_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFP9947017.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHOT6_001786"
     CDS             complement(27724..29013)
                     /gene="purD"
                     /locus_tag="ACHOT6_001786"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFP9947018.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVEDGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHOT6_001787"
     CDS             complement(29025..30614)
                     /gene="purH"
                     /locus_tag="ACHOT6_001787"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFP9947019.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7323 a         8043 c         8477 g         7176 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gtagcaaaag ttctgagcta attgccgata
      241 acatttgacg caatgcgcac taaaagggca tcatttgatg ccctttttgc acgctttcgt
      301 accagaacct ggctcatcag tgattttctt tgtcataatc attgctgaga caggctctgt
      361 tgagggcgta taatccgaaa agctaatacg cgtttcgatt tggtttgcct cgcgatcgcg
      421 gggtgaaaat gtttgtagaa aacttctgac aggttggttt atgagtgcga ataccgaagc
      481 tcaaggaagc gggcgcggcc tggaagcgat gaagtgggtc gttgtggtgg cattgctcct
      541 ggtggcgatt gtcggcaact acctttatcg cgacattatg ctgccgctgc gtgcgctggc
      601 cgtagtaatt ctgattgctg cagcgggtgg tgtcgcgctg ttaacgacaa aaggtaaagc
      661 taccgttgct tttgcccgtg aagcgcgtac cgaagtccgt aaggtcattt ggccgactcg
      721 ccaggaaaca ttgcacacca cgctgattgt ggctgcggtt accgcagtaa tgtcactgat
      781 cctgtgggga ctggatggta ttctggttcg cctggtatcc tttatcactg gcctgaggtt
      841 ctgagatgtc tgaagctcct aaaaagcgct ggtacgtcgt tcaggcgttt tccggttttg
      901 aaggccgcgt agcaacgtcg ctgcgtgagc atatcaaatt acacaacatg gaagatttgt
      961 ttggtgaagt catggtacca accgaagaag tggttgaaat ccgtggcggt cagcgtcgca
     1021 aaagcgaacg taaattcttc cctggctacg tcctcgttca gatggtgatg aacgacgcga
     1081 gctggcacct ggtgcgcagc gtaccgcgtg tgatgggctt catcggcggt acttccgatc
     1141 gtcctgcgcc aatcagcgat aaagaagtcg atgcgattat gaaccgcctg cagcaggttg
     1201 gtgataagcc gcgtccgaaa acgctgtttg aaccgggtga aatggtccgt gttaatgatg
     1261 gtccgttcgc tgacttcaac ggtgttgttg aagaagtgga ttacgagaaa tctcgtctga
     1321 aagtgtctgt ttctatcttc ggtcgtgcga ctccggtaga gctggacttc agccaggttg
     1381 aaaaagccta acccagcgtt caaaaaagcg gcgatttaat cgttgcacaa ggcgtgagat
     1441 tggaatacaa tttcgcgcct tttgttttta tgggccttgc ccgtaaaacg attttttata
     1501 tcacggggag cctctcagag gcgttattac ccaacttgag gaatttataa tggctaagaa
     1561 agtacaagcc tatgtcaagc tgcaggttgc agctggtatg gctaacccga gtccgccagt
     1621 aggtccggct ctgggtcagc agggcgtaaa catcatggaa ttctgcaaag cgttcaacgc
     1681 aaaaactgat tccatcgaaa aaggtctgcc gattccggta gtaatcaccg tttacgctga
     1741 ccgttctttc actttcgtta ccaagactcc gccggcagca gttctgctga aaaaagcggc
     1801 tggtatcaag tctggttccg gtaagccgaa caaagacaaa gtgggtaaaa tttcccgcgc
     1861 tcagctgcag gaaatcgcgc agaccaaagc tgccgacatg actggtgccg acattgaagc
     1921 gatgactcgc tccatcgaag gtactgcacg ttccatgggc ctggtagtgg aggactaaga
     1981 aatggctaaa ctgaccaagc gcatgcgtgt tatccgcgag aaagttgatg caaccaaaca
     2041 gtacgacatc aacgaagcta tcgcactgct gaaagagctg gcgactgcta aattcgtaga
     2101 aagcgtggac gtagctgtta acctcggcat cgacgctcgt aaatctgacc agaacgtacg
     2161 tggtgcaact gtactgccgc acggtactgg ccgttccgtt cgcgtagccg tatttaccca
     2221 aggtgcaaac gctgaagctg ctaaagctgc aggcgcagaa ctggtaggta tggaagatct
     2281 ggctgaccag atcaagaaag gcgaaatgaa ctttgacgtt gttattgctt ctccggatgc
     2341 aatgcgcgtt gttggccagc tgggccaggt tctgggtccg cgcggcctga tgccaaaccc
     2401 gaaagtgggt actgtaacac cgaacgttgc tgaagcggtt aaaaacgcta aagctggcca
     2461 ggttcgttac cgtaacgaca aaaacggcat catccacacc accatcggta aagtggactt
     2521 tgacgctgac aaactgaaag aaaacctgga agctctgctg gttgcgctga aaaaagcaaa
     2581 accgactcag gcgaaaggcg tgtacatcaa gaaagttagc atctccacca ccatgggtgc
     2641 aggtgttgca gttgaccagg ctggcctgag cgcttctgta aactaatgcc tttacgtggg
     2701 cggtgatttt gtctacaatc ttacccccac gtataatgct taatgcagac gtatatccga
     2761 gatattcggg ttgtggcaag gcggcaactg agtgagtcgc caggagcata gctaactatg
     2821 tgactggtgc gaatgaagga agccaacgcc gccacaagtt gaatagcgac ggatagaaaa
     2881 gatttgttcg ttggagcctg gcctatccag gcctccgtcg aagaccgcag gagtttcgca
     2941 agaaacttaa tcccctgcgt agacggtgac agaacgctaa gattattctt ttatattctg
     3001 gcttgtttct gctcaccgta attaagacgc tctctccgtt tggaggagtg aagtgagttc
     3061 cagagatttt ctctggcaaa catccaggag caaagctaat ggctttaaat cttcaagaca
     3121 aacaagcgat tgttgctgaa gtcagcgaag tagccaaagg cgcgctgtct gcagtagttg
     3181 cggattcccg tggcgtaact gtagataaaa tgactgaact gcgtaaagca ggtcgcgaag
     3241 ctggcgtata catgcgtgtt gttcgtaaca ccctgctgcg ccgtgctgtt gaaggtactc
     3301 cgttcgagtg cctgaaagac gcgtttgttg gtccgaccct gattgcatac tctatggaac
     3361 acccgggcgc tgctgcccgt ctgttcaaag agttcgcgaa agcgaatgca aaatttgagg
     3421 tcaaagccgc tgcctttgaa ggtgagctga tcccggcgtc tcagatcgac cgcctggcaa
     3481 ctctgccgac ctacgaagaa gcaattgcac gcctgatggc aaccatgaaa gaagcttcgg
     3541 ctggcaaact ggttcgtact ctggctgctg tacgcgatgc gaaagaagct gcttaatcgc
     3601 agttatcttt ttaacgcatt cgcttacgta taaacttatt ctgatattca ggaacaattt
     3661 aaatgtctat cactaaagat caaatcattg aagcagttgc agctatgtct gtaatggacg
     3721 ttgtagaact gatctctgca atggaagaaa aattcggtgt ttccgctgct gctgctgtag
     3781 ctgtagctgc tggcccggtt gaagctgctg aagaaaaaac tgaattcgac gtaattctga
     3841 aagctgctgg cgctaacaaa gttgctgtta tcaaagcagt acgtggcgca actggcctgg
     3901 gtctgaaaga agctaaagac ctggtagaat ctgcaccggc tgctctgaaa gaaggcgtga
     3961 gcaaagacga cgcagaagca ctgaaaaaag ctctggaaga agctggcgct gaagttgaag
     4021 ttaaataagc caacccttcc ggttgcagcc tgagaaatca ggctgatggc tggtgacttt
     4081 ttagtcacca gcctttttgc gctgtaaggc gccagtagcg tttcacactg tttgactact
     4141 gctgtgcctt tcaatgcttg tttctatcga cgacttaata tactgcgaca ggacgtccgt
     4201 tctgtgtaaa tcgcaatgaa atggtttaag cgtgatagca acaggcattg cggaaagtgt
     4261 tccattttcc ggtcaacaaa atagtgttgc acaaactgtc cgctcaatgg acagatgggt
     4321 cgacttgtca gcgagctgag gaaccctatg gtttactcct ataccgagaa aaaacgtatt
     4381 cgtaaggatt ttggtaaacg tccacaagtt ctggatgtac cttatctcct ttctatccag
     4441 cttgactcgt ttcagaaatt tatcgagcaa gatcctgaag ggcagtatgg tctggaagct
     4501 gctttccgtt ccgtattccc gattcagagc tacagcggta attccgagct gcaatacgtc
     4561 agctaccgcc ttggcgaacc ggtgtttgac gtccaggaat gtcaaatccg tggcgtgacc
     4621 tattccgcac cgctgcgcgt taaactgcgt ctggtgatct atgagcgcga agcgccggaa
     4681 ggcaccgtaa aagacattaa agaacaagaa gtctacatgg gcgaaattcc gctcatgaca
     4741 gacaacggta cctttgttat caacggtact gagcgtgtta tcgtttccca gctgcaccgt
     4801 agtccgggcg tcttctttga ctccgacaaa ggtaaaaccc actcttcggg taaagtgctg
     4861 tataacgcgc gcatcatccc ttaccgtggt tcctggctgg acttcgaatt cgatccgaag
     4921 gacaacctgt tcgtacgtat cgaccgtcgc cgtaaactgc ctgcgaccat cattctgcgc
     4981 gccctgaact acaccacaga gcagatcctc gacctgttct ttgaaaaagt tatctttgaa
     5041 atccgtgata acaagctgca gatggaactg gtgccggaac gcctgcgtgg tgaaaccgca
     5101 tcttttgaca tcgaagctaa cggtaaagtg tacgtagaaa aaggccgccg tatcactgcg
     5161 cgccacattc gccagctgga aaaagacgac gtcaaactga tcgaagtccc ggttgagtac
     5221 atcgcaggta aagtggttgc taaagactat attgatgagt ctaccggcga gctgatctgc
     5281 gcagcgaaca tggagctgag cctggatctg ctggctaagc tgagccagtc tggtcacaag
     5341 cgtatcgaaa cgctgttcac caacgatctg gatcacggcc catatatctc tgaaacctta
     5401 cgtgtcgacc caactaacga ccgtctgagc gcactggtag aaatctaccg catgatgcgc
     5461 cctggcgagc cgccgactcg tgaagcagct gaaagcctgt tcgagaacct gttcttctcc
     5521 gaagaccgtt atgacttgtc tgcggttggt cgtatgaagt tcaaccgttc tctgctgcgc
     5581 gaagaaatcg aaggttccgg tatcctgagc aaagacgaca tcattgatgt tatgaaaaag
     5641 ctcatcgata tccgtaacgg taaaggcgaa gtcgatgata tcgaccacct cggcaaccgt
     5701 cgtatccgtt ccgttggcga aatggcggaa aaccagttcc gcgttggcct ggtacgtgta
     5761 gagcgtgcgg tgaaagagcg tctgtctctg ggcgatctgg ataccctgat gcctcaggat
     5821 atgatcaacg ccaagccgat ttccgcagca gtgaaagagt tcttcggttc cagccagctg
     5881 tctcagttta tggaccagaa caacccgctg tctgagatta cgcacaaacg tcgtatctcc
     5941 gcactcggcc caggcggtct gacccgtgaa cgtgcaggct tcgaagttcg agacgtacac
     6001 ccgactcact acggtcgcgt atgtccaatc gaaacccctg aaggtccgaa catcggtctg
     6061 atcaactctc tgtccgtgta cgcacagact aacgaatacg gcttccttga gactccgtat
     6121 cgtaaagtga ccgacggtgt tgtaactgac gaaattcact acctgtctgc tatcgaagaa
     6181 ggcaactacg ttatcgccca ggcgaactcc aacctggatg aagaaggcca cttcgtagaa
     6241 gacctggtaa cttgccgtag caaaggcgaa tccagcttgt tcagccgcga ccaggttgac
     6301 tacatggacg tatccaccca gcaggtggta tccgtcggtg cgtccctgat cccgttcctg
     6361 gaacacgatg acgccaaccg tgcattgatg ggtgcgaaca tgcaacgtca ggccgttccg
     6421 actctgcgcg ctgataagcc gctggttggt actggtatgg aacgtgctgt tgccgttgac
     6481 tccggtgtaa ctgcggtagc taaacgtggt ggtgtcgttc agtatgtgga tgcttcccgt
     6541 atcgttatca aagttaacga agacgagatg tatccgggtg aagcaggtat cgacatctac
     6601 aacctgacca aatacacccg ttctaaccag aacacctgta tcaaccagat gccgtgtgtg
     6661 tctctgggtg aaccggttga acgtggcgac gtgctggcag acggtccgtc caccgacctc
     6721 ggtgaactgg cgcttggtca gaacatgcgc gtagcgttca tgccgtggaa tggttacaac
     6781 ttcgaagact ccatcctcgt atccgagcgt gttgttcagg aagaccgttt caccaccatc
     6841 cacattcagg aactggcgtg tgtgtcccgt gacaccaagc tggggccgga agagatcacc
     6901 gctgacatcc cgaacgtggg tgaagctgcg ctctccaaac tggatgaatc cggtatcgtt
     6961 tacattggtg cggaagtgac cggtggcgac attctggttg gtaaggtaac gccgaaaggt
     7021 gaaactcagc tgaccccaga agaaaaactg ctgcgtgcga tcttcggtga gaaagcctct
     7081 gacgttaaag actcttctct gcgcgtacca aacggtgtat ccggtacggt tatcgacgtt
     7141 caggtcttta ctcgcgatgg cgtagaaaaa gacaaacgtg cgctggaaat cgaagaaatg
     7201 cagctcaaac aggcgaagaa agacctgtct gaagaactgc agatcctcga agcgggtctg
     7261 ttcagccgta tccgtgctgt gctggtagcc ggtggcgttg aagctgagaa gctcgacaaa
     7321 ctgccgcgcg atcgctggct ggagctgggc ctgacagacg aagagaaaca aaatcagctg
     7381 gaacagctgg ctgagcagta tgacgaactg aaacacgagt tcgagaagaa actcgaagcg
     7441 aaacgccgca aaatcaccca gggcgacgat ctggcaccgg gcgtgctgaa gattgttaag
     7501 gtatatctgg cggttaaacg ccgtatccag cctggtgaca agatggcagg tcgtcacggt
     7561 aacaagggtg taatttctaa gatcaacccg atcgaagata tgccttacga tgaaaacggt
     7621 acgccggtag acatcgtact gaacccgctg ggcgtaccgt ctcgtatgaa catcggtcag
     7681 atcctcgaaa cccacctggg tatggctgcg aaaggtatcg gcgacaagat caacgccatg
     7741 ctgaaacagc agcaagaagt cgcgaaactg cgcgaattca tccagcgtgc gtacgatctg
     7801 ggcgctgacg ttcgtcagaa agttgacctg agtaccttca gcgatgaaga agttatgcgt
     7861 ctggctgaaa acctgcgcaa aggtatgcca atcgcaacgc cggtgttcga cggtgcgaaa
     7921 gaagcagaaa ttaaagagct gctgaaactt ggcgacctgc cgacttccgg tcagatccgc
     7981 ctgtacgatg gtcgcactgg tgaacagttc gagcgtccgg taaccgttgg ttacatgtac
     8041 atgctgaaac tgaaccacct ggtcgacgac aagatgcacg cgcgttccac cggttcttac
     8101 agcctggtta ctcagcagcc gctgggtggt aaggcacagt tcggtggtca gcgtttcggg
     8161 gagatggaag tgtgggcgct ggaagcatac ggcgcagcat acaccctgca ggaaatgctc
     8221 accgttaagt ctgatgacgt gaacggtcgt accaagatgt ataaaaacat cgtggacggc
     8281 aaccatcaga tggagccggg catgccagaa tccttcaacg tattgttgaa agagattcgt
     8341 tcgctgggta tcaacatcga actggaagac gagtaattct cgctcaaaca ggtcactgct
     8401 gtcgggttaa aacccggcag cggattgtgc taactccgac gggagcaaat ccgtgaaaga
     8461 tttattaaag tttctgaaag cgcagactaa aaccgaagag tttgatgcga tcaaaattgc
     8521 tctggcttcg ccagacatga tccgttcatg gtctttcggt gaagttaaaa agccggaaac
     8581 catcaactac cgtacgttca aaccagaacg tgacggcctt ttctgcgccc gtatctttgg
     8641 gccggtaaaa gattacgagt gcctgtgcgg taagtacaag cgcctgaaac accgtggcgt
     8701 catctgtgag aagtgcggcg ttgaagtgac ccagactaaa gtacgccgtg agcgtatggg
     8761 ccacatcgaa ctggcttccc cgactgcgca catctggttc ctgaaatcgc tgccgtcccg
     8821 tatcggtctg ctgctcgata tgccgctgcg cgatatcgaa cgcgtactgt actttgaatc
     8881 ctatgtggtt atcgaaggcg gtatgaccaa cctggaacgt cagcagatcc tgactgaaga
     8941 gcagtatctg gacgcgctgg aagagttcgg tgacgaattc gacgcgaaga tgggggcgga
     9001 agcaatccag gctctgctga agagcatgga tctggagcaa gagtgcgaac agctgcgtga
     9061 agagctgaac gaaaccaact ccgaaaccaa gcgtaaaaag ctgaccaagc gtatcaaact
     9121 gctggaagcg ttcgttcagt ctggtaacaa accagagtgg atgatcctga ccgttctgcc
     9181 ggtactgccg ccagatctgc gtccgctggt tccgctggat ggtggtcgtt tcgcgacttc
     9241 tgacctgaac gatctgtatc gtcgcgtcat taaccgtaac aaccgtctga aacgtctgct
     9301 ggatctggct gcgccggaca tcatcgtacg taacgaaaaa cgtatgctgc aggaagcggt
     9361 agacgccctg ctggataacg gtcgtcgcgg tcgtgcgatc accggttcta acaagcgtcc
     9421 tctgaaatct ttggccgaca tgatcaaagg taaacagggt cgtttccgtc agaacctgct
     9481 cggtaagcgt gttgactatt ccggtcgttc tgtaatcacc gtaggtccat acctgcgtct
     9541 gcatcagtgc ggtctgccga agaaaatggc gctggagctg ttcaaaccgt tcatctacgg
     9601 caagctggaa ctgcgtggtc ttgctaccac cattaaagct gcgaagaaaa tggttgagcg
     9661 cgaagaagct gtcgtttggg atatcctgga cgaagttatc cgcgaacacc cggtactgct
     9721 gaaccgtgca ccgactctgc accgtctggg tatccaggca tttgaaccgg tactgatcga
     9781 aggtaaagct atccagctgc acccgctggt ttgtgcggca tataacgccg acttcgatgg
     9841 tgaccagatg gctgttcacg taccgctgac gctggaagcc cagctggaag cgcgtgcgct
     9901 gatgatgtct accaacaaca tcctgtcccc ggcgaacggc gaaccaatca tcgttccgtc
     9961 tcaggacgtt gttctgggtc tgtactatat gacccgtgac tgtgttaacg ccaaaggcga
    10021 aggcatggtg ctgactggcc cgaaagaagc agaacgtctg tatcgctctg gtctggcttc
    10081 tctgcatgcg cgcgttaaag tgcgtatcac cgagtatgaa aaagatgcaa acggtgaatt
    10141 agtagcgaaa accagcctga aagacacgac tgttggccgt gccattctgt ggatgattgt
    10201 accgaaaggt ctgccttact ccatcgtcaa ccaggcgctg ggtaaaaaag caatctccaa
    10261 aatgctgaac acctgctacc gcattctcgg tctgaaaccg accgttattt ttgcggacca
    10321 gatcatgtac accggcttcg cctatgcagc gcgttctggt gcatctgttg gtatcgatga
    10381 catggtcatc ccggagaaga aacacgaaat catctccgag gcagaagcag aagttgctga
    10441 aattcaggag cagttccagt ctggtctggt aactgcgggc gaacgctaca acaaagttat
    10501 cgatatctgg gctgcggcga acgatcgtgt atccaaagcg atgatggata acctgcaaac
    10561 tgaaaccgtg attaaccgtg acggtcagga agagaagcag gtttccttca acagcatcta
    10621 catgatggcc gactccggtg cgcgtggttc tgcggcacag attcgtcagc ttgctggtat
    10681 gcgtggtctg atggcgaagc cggatggctc catcatcgaa acgccaatca ccgcgaactt
    10741 ccgtgaaggt ctgaacgtac tccagtactt catctccacc cacggtgctc gtaaaggtct
    10801 ggcggatacc gcactgaaaa ctgcaaactc cggttacctg actcgtcgtc tggttgacgt
    10861 ggcgcaggac ctggtggtta ccgaagacga ttgtggtacc catgaaggta tcatgatgac
    10921 tccggttatc gaaggtggtg atgttaaaga gccgctgcgc gatcgcgtac tgggtcgtgt
    10981 aactgctgaa gacgttctga agccgggtac tgctgatatc ctcgttccgc gcaacacgct
    11041 gctgcacgaa cagtggtgtg acctgctgga agagaactct gtcgacgcgg ttaaagtacg
    11101 ttctgttgta tcttgtgaca ccgactttgg tgtatgtgcg cactgctacg gtcgtgacct
    11161 ggcgcgtggc cacatcatca acaagggtga agcaatcggt gttatcgcgg cacagtccat
    11221 cggtgaaccg ggtacacagc tgaccatgcg tacgttccac atcggtggtg cggcatctcg
    11281 tgcggctgct gaatccagca tccaggtgaa aaacaaaggt agcatcaagc tcagcaacgt
    11341 gaagtcggtt gtgaactcca gcggtaaact ggttatcact tcccgtaata ctgaactgaa
    11401 actgatcgac gaattcggtc gtaccaaaga aagctacaaa gtaccttacg gtgcggtact
    11461 ggcgaaaggc gatggcgaac aggttgctgg cggcgaaacc gttgcaaact gggacccgca
    11521 caccatgccg gttatcaccg aagtaagcgg ttttgtacgc tttactgaca tgatcgacgg
    11581 tcagaccatt actcgtcaga cagacgaatt gaccggtctg tcttcgctgg tggttctgga
    11641 ttccgcagaa cgtaccgcag gtggtaaaga tctgcgtccg gcactgaaaa tcgttgatgc
    11701 tcagggtaac gacgttctga tcccaggtac cgatatgcct gcgcagtact tcctgccggg
    11761 taaagcgatt gttcagctgg aagatggcgt acagatcagc tctggtgaca ccctggcgcg
    11821 tattccgcag gaatccggcg gtaccaagga catcaccggt ggtctgccgc gcgttgcgga
    11881 cctgttcgaa gctcgtcgtc cgaaagagcc ggcaatcctg gctgaaatca gcggtatcgt
    11941 ttccttcggt aaagaaacca aaggtaaacg tcgtctggtt atcaccccgg tagacggtag
    12001 cgatccgtac gaagagatga ttccgaaatg gcgtcagctc aacgtgttcg aaggtgaacg
    12061 tgtagaacgt ggtgacgtaa tttccgacgg tccggaagcg ccgcacgaca ttctgcgtct
    12121 gcgtggtgtt catgctgtga ctcgttacat cgttaacgaa gtacaggacg tataccgtct
    12181 gcagggcgtt aagattaacg ataaacacat cgaagttatc gttcgtcaga tgctgcgtaa
    12241 agctaccatc gttaacgcgg gcagctccga cttcctggaa ggcgaacagg ttgaatactc
    12301 tcgcgtcaag atcgcaaacc gcgaactgga agcgaacggc aaagtgggtg caacttactc
    12361 ccgcgatctg ctgggtatca ccaaagcgtc tctggcaacc gagtccttca tctccgcggc
    12421 atcgttccag gagaccactc gcgtgctgac cgaagcagcc gttgcgggca aacgcgacga
    12481 actgcgcggc ctgaaagaga acgttatcgt gggtcgtctg atcccggcag gtaccggtta
    12541 cgcgtaccac caggatcgta tgcgtcgccg tgctgcgggt gaagctccgg ctgcaccgca
    12601 ggtgactgca gaagacgcat ctgccagcct ggcagaactg ctgaacgcag gtctgggcgg
    12661 ttctgataac gagtaatcgt taatccgcaa ataacgtaaa aacccgcttc ggcgggtttt
    12721 tttatggggg gagtttaggg aaagagcatt tgtcagaata tttaaggaat ttctgaatac
    12781 tcataatcaa tgtagagatg actaatatcc tgaaactgac tgaactaatt gagtcaaact
    12841 cggcaaggat tcgatactat tcctgtgtaa ctttcttaag gaacgagaat gaaacaggaa
    12901 gtggaaaagt ggcgaccttt tggacatccg gatggtgata ttcgtgattt atcatttctt
    12961 gatgctcatc aggctgtcta cgttcagcat catgagggca aagagccttt agagtatcgc
    13021 ttttgggtta cctactctct tcactgcttc acaaaagatt atgaacatca gacgaacgaa
    13081 gaaaaacaat cgttaatgta ccacgcgcct aaagaatctc gtcccttctg ccagcaccgt
    13141 tataacttag cgcgcacaca cttaaaaaga actattttgg cgctgccaga aagcaacgtt
    13201 attcatgccg ggtatggtag ctatgccgtg attgaggtgg acttagacgg aggagataag
    13261 gcattttact ttgttgcgtt cagggctttc agggaaaaga aaaaactccg tttgcatgta
    13321 actagcgctt atcccatttc tgaaaaacag aaaggtaaat cagtgaaatt tttcaccatt
    13381 gcctacaact tattgagaaa taagcagctt cctcagccct caaaataaca aaacccacct
    13441 taaggtgggt ttcgccagag aattatctct ggtattcaga acgccattac cggactttgc
    13501 cttgaccttg cgataatcgc aggttgcggg atgtctgaat ttcttcagtc tgctgcatcc
    13561 tggaagatga gaacatgtgt tcttattttc gtctctatca tagttgagta tttactctct
    13621 tacaatcaga tctctttcat tgctcaacag gcgatggctt cagactttgc attacggaat
    13681 ttttaagaaa ggcagggcga aacgaggaag aagcttttcg ccctggacaa cattcctgct
    13741 gacggcacta ccataaaaaa acagaacgct ttcaacaacc ttgcgttttt catcttccgt
    13801 agcggctcgg aataaattta cgttcttaca ttccgtctca tagtctttgc gaggcccgtc
    13861 ccagatagct gtcccagtct ttccataccg gctgcaaacc ctgagcggtt aacgcggcag
    13921 caaccgcttc cggtctgcga tcgtcgtgcg gtgagaactg ttccaactcg gggtgattat
    13981 cggcatagcc acctggctgc gttttcgaga aggcgctgac gttattgatc gccagcggaa
    14041 taacgcgatc gcgaaaccac ggtgattccc gcgtggagag tgacagttca atctccggtg
    14101 caagcagtcg gaaggcgcag atggtttgca ctaactggcg ttcatccata atcgacgcag
    14161 gctcaatgcc gccagtacac gggcgcaggc gcggaaagga gacagagtaa cggctttgcc
    14221 agtaatgctg ttgcagccat agcaaatgtt cggcaaccat atagcagtca acgcgccagt
    14281 tgtcggaaag gccaattagc gcgccgaggc ctatcttatc aatccccgca cgccccagcc
    14341 gatccggcgt ttccagccgc cagaagaagt cctgtttttt gcctttcaga tgatggcggg
    14401 cataagtcgc ctcgtgatat gtctcctgat aaaccatcac gccatccaga ccaagctgct
    14461 ttaactcggc gtattccgtc tccgccagcg gttgcacttc catttgtagt gaagagaact
    14521 gttcacgcag ggcggggaga tgacgacgaa agtaatccat ccccactttc gcctgatgtt
    14581 caccagtgac taatagcaga tgttcaaagc ccatctcccg tatagcggcg ctttccctgg
    14641 caatatccgc ttcatccagc gttttgcgct tgatgcgatt actcatggaa aatccgcagt
    14701 acgtgcagtc gttagcgcaa agattggaaa gataaagcgg gacgtagaaa ctaactgtgt
    14761 tgccaaatcg ctgacgggtc agacgctgcg cccgttgggc cagttgttcc agatagccac
    14821 tggcggctgg cgataacagc gccatcatat cgtcgcgggt gaattgcgag gcatttagcg
    14881 cccgctctac gtcaacagcc gttttgccgt tgatacgcag gcggatgtca tcccagtcca
    14941 gttgtcgcca gcgatcgctg aaggttttca tgccgatgcc tccagaaatc cagtcagcgg
    15001 gctggtggca tgagcaaaat gactgcggct gcccggtccg gactgacgtg ccagcaggcc
    15061 tgcttctacc gccagacgaa atgccttcgc catgttgacg ggatcgtccg ctacggcaat
    15121 cgccgtattc actaacaccg cgtcggcccc catttccagg gcctgcgcgg catggctggg
    15181 aacgccgatg ccagcatcga caaccaccgg aactgtggcc tgctggataa taatctccag
    15241 catggcacag gtttccagtc cctgattcga gccaatcggc gcgccgagcg gcatcaccgc
    15301 tgcacagccg acttcttcta gacgtttaca caataccgga tcggccccgc agtaaggcag
    15361 cacgacaaat ccctgttgta ccagcatttc ggcggctttc agagtttcga tgggatcggg
    15421 caacagccag cgggcgtcag ggtgaatctc caattttagc cagttagtgc ctaacgcttc
    15481 acgtgccaga tgggcggcga aaatggcttc ttccgccgtt ttcgccccgg aagtattcgg
    15541 cagcagggtc acacccgccg cgataagcgg ttcgaggata gcgtcgttgt gctggcgcaa
    15601 gtcgacacgt ttcatcgcca gtgtcaccag ctggctgccg gaagcgcgga tcgcctccat
    15661 catcagttgt gaagaagcga atttccctgt gccggtaaac agatgtgaat caaacgtttt
    15721 gtccgcaata cgtaacatct caaccccctg caataacctg aaaaagcagg atctggtcgc
    15781 catcctgcac gatatgttgc gtccactgct cacgcgggac gatttgctga ttaatcgcca
    15841 gagccgcgcc cgcttgtcgt tggtcgagtt gctccagtag ttcgtgaaca gtttgcccgg
    15901 cggcgcactg catcggttga tcgttaaaca ggatctgcat tgcttcctcc gcataccggg
    15961 caaccactgg cgcggcgcaa cgccaggctg cgccactggc tcgatttacc gtcgaacagt
    16021 cggagttctc ccgcaggtgt ctctataccg cttaataact taatggcttc cagtgcctgc
    16081 aaagtgccca taaccccgac caccgggcca accacgcccg ccgtgcggca gttgcgttct
    16141 ggctcctggt tatctggcca caggcagcgg taacacccct gctcccaggg cggcgtcagt
    16201 accatcaact gaccgccaaa tccgaccgcg ctggcggtga taagcggcgt gttgagtacc
    16261 acgcaggcgg cattaatctt ctggcgagtc gccatattgt cggtacagtc gagcaccaca
    16321 tcggcccgtg caaccgcatc ttttaacgcc tcacccgtta accgttgttg taatgctgtc
    16381 agttgaatgt cgggattcaa ctgtgtcagt cgctgttggc tgacctgcga tttcgggcga
    16441 tcgatatctt cagtggtaaa gaggatttgt cgttgcagat tgcttaaatg cacatcgtcg
    16501 tcatctgcca gtaccagcgt cccgacgcca gcgcccgcta ggtatagcgc agcaggtgta
    16561 cccagcccgc caagaccgat aatcagcacc tggctgtcga gcagtttttg ctgcccgtca
    16621 agagcgatat cgtcgagcag gatttggcgg ctataacgca taaaatcacg gtcattcatc
    16681 gccaactcct gcaatttcca gcaactgtgc cgttgccaaa cgccagtctg cggcttgagt
    16741 aatggcgctg acgacggcga tactgccgac acccgttgct atcaccgcag gcgcgcgtgc
    16801 cagactgaca ccgccaatcg ccacggtggg ataatccgcc agtcgctcaa catgccgtgc
    16861 cagctgttcc agcccctgcg gtgcagaagg catctgtttg gtttgcgtcg ggaacacatg
    16921 tcccagcgcg atataagagg ggcgtgctgc cagcgcgacg tcgatttcca tatcgtcatg
    16981 tgtcgaaacg cccagccgca ggcctgccgc gcggatggca ttgagatcgg tggcttgcaa
    17041 atcttcctgc cccaaatgga cgccatacgc ctgatgcttg atcgccagcc gccagtaatc
    17101 gttgataaac aatcgcgcgt tatagcggcg gcccagcgca attgccgcca cgacatcggc
    17161 ttccacctct tcatcgcgcc gatctttgat gcgtagctgg agagtacgta cgcctgcatc
    17221 caacagacgt tcgatccact gtacgctgtc caccaccggg tacagtccta aacgaaaagg
    17281 tacaggagga aaatcaggct gatacatcac gcttcctcct tacgcaggta gatttctccg
    17341 cctctggcac ggaagttctc cgacatatcc gccattccca tttcaatagt ttgcgtggcg
    17401 gcgtaatcac gcacttcctg gctgattttc atcgagcaga atttcggccc acacatggag
    17461 caaaaatggg cgactttacc tgactcttgc ggcagggttt catcgtgata agcgcgggcg
    17521 gtaaacgggt cgagggccag attaaactgg tcttcccagc gaaattcgaa gcgggctttc
    17581 gacatggcgt tatcgcgaat ttgcgcgccc ggatgccctt tcgccaggtc agcggcgtgg
    17641 gcggcaatct tataggtgat aagcccctgc ttaacatctt ctttattagg cagacccaga
    17701 tgctcttttg gcgttacgta acagagcatc gcgcagccaa accagccaat catcgccgca
    17761 ccaatccccg acgtgaagtg gtcatagccc ggcgcaatat cggtagttag cggccccaga
    17821 gtgtaaaacg gcgcttcgtg gcagtgctct aactcctcgg tcatattgcg gcggatcatc
    17881 tgcatcggca cgtggcctgg gccttcaatc atcacctgca cgtcatattc ccaggcaatt
    17941 ttggtcagtt cgcccagcgt atgcagctcg gcaaactgcg cttcatcgtt ggcgtcctga
    18001 atagaaccgg ggcgcagacc gtcgcccagc gacagcgaaa cgtcataagc ggcacagatt
    18061 tcacaaattt cgcggaagtg ttgatagagg aaattttcct gatgatggga gaggcaccat
    18121 ttcgccataa tcgaaccgcc gcgagagacg ataccggtca ggcgtttcgc ggtcatcggc
    18181 acatagcgca gcagtacgcc cgcatggata gtgaagtaat ccacaccttg ctcggcctgt
    18241 tccagcagcg tgtcgcggaa cgcttcccag gtaagatctt cggcgatccc gttaaccttc
    18301 tccagcgcct ggtagatcgg cactgtaccg atcggcaccg ggctgttacg caaaatccac
    18361 tcgcgggttt cgtgaatata gcgaccggtg gagagatcca tcaccgtatc cgctccccag
    18421 cgcgtggacc ataccagctt ttccacttct tcttcgatgg aagaggtgac cgccgagttg
    18481 ccgatattgg cgttaacttt taccaggaaa ttgcgaccaa taatcatcgg ctccgattcc
    18541 ggatgattaa tgttggccgg gataatcgca cgtccggcag caacttcatc acggacaaat
    18601 tccgcagtga tattttccgg cagatgtgcg ccaaagctca ttcccggatg ctggtggcgt
    18661 aaaacctcgc tacggatgcg ctcgcggccc atattctcgc ggatggcgat gaattccatt
    18721 tccggcgtga tgatgccctg gcgggcgtag tgcagttggg tgacacggcg tcctgctttg
    18781 gcgcgttttg gtgttagtac gccgctaaaa cgcagttcgt cgaggccatc atctgccagc
    18841 cgcgctttag tgtaatcgga actgcgcacg gtaagttctt cggtatcgcc gcgcgcatcg
    18901 atccacggct ggcgtagttt tgccagccct tgctgcacgt taatggcaat ctgcggatca
    18961 ccatacgggc cggaggtgtc gtagaccgga atcgcttcgt tttcttcgta ctgcggctgt
    19021 tctttgctac cgccaattag cgtcgggcta agctggatct cacgcatcgg cacgcgcacg
    19081 ccggggtgtg tgccagtgat ataaatgcgt tttgagttgg gaaaggcggt gccttccagg
    19141 gtgtcgataa aatgttgggc ccgggcgcgt tgttcgcggc gggtcagttt tgttgcagac
    19201 atagctcatt ccaaaaagtt aaggacgtgg cttgtcagac gacggatgaa gcaagagacg
    19261 atcgccgcag gggcgatgcg atagcagatt aactcttgtt cccttcgcag gtattagcct
    19321 gatcaggttc cgcggatccc gaataaacgg tctcagccag ttaaggcact ccgacaagaa
    19381 attagccccg caaatggggc attgaatgta aattacgcgt taacagcgca gaactcaagc
    19441 aggatgtttg acgcgggcgg cgtcaagcac cagcagcatc agcttatctt ccagcacaaa
    19501 gcgcgcttcc agcgcctcgc cgatgtcaga taaaacctgt tgaaactcaa ggtaattatc
    19561 atgatcgatg gcggtttcca gactggaatc gtagtaatcc ataatctgtt gagtattggc
    19621 ttcgagttgc ggccaaatct tcgcggcgcg cgcgagttgc ccgttgcctt ccagcttatg
    19681 aagaatgcgc tcataaatac tgaaatgtcc ggcagacaag taatcgacca ggctctgaca
    19741 aaaatcatca agggcttttt cgtttagcct catgtacgat tctttgccag gcttaatgcc
    19801 aaccagattg tagtaagcca cgagcagatg cttacgtaca tgtagccagc gatcaaccag
    19861 tttgttactt cctctgacgc gctccgtcag gttatcgagc tggttaagca tgattgactc
    19921 cgcaagtttg tattcaaaaa ctgctcagtg agaaatgtaa aaaccatgtt aaacatgcca
    19981 gtgatgcaaa ggtagtgcaa gagctatgga tcgtataatt gaaaaattag atcacggctg
    20041 gtgggtcgtc agccatgaac aaaaattatg gttgccgaag ggagaattgc catatggcga
    20101 agcggcaaat ttcgatcttg tgggtcagcg cgcactacaa atcggcgaat ggcaggggga
    20161 acctgtttgg ttagtacaac agcagcggcg tcacgatatg gggtcggtac gtcaggtcat
    20221 tgatctcgat gttgggctgt ttcaactggc cggacgaggc gtacaactgg cggagtttta
    20281 ccgatcgcat aaatactgtg gttactgcgg gcatgaaatg tatccgagca aaaccgaatg
    20341 ggcgatgctg tgcagccatt gccgtgagcg ttactacccg caaatcgccc cctgcattat
    20401 tgttgccatc cgtcgcgatg attcgatcct cctcgcccag catacccgcc atcgtaacgg
    20461 tgtccataca gtacttgccg gattcgtcga agtgggcgaa accctcgagc aggcagtcgc
    20521 gcgggaagtg atggaagaga gcggaattaa agttaaaaac ttgcgttacg tgacttctca
    20581 gccgtggccg tttcctcagt ctttaatgac cgcgtttatg gcggaatatg acagcggcga
    20641 catcgtgatc gacccgaaag aattgctcga ggcgaactgg tatcgctatg acgatttgcc
    20701 gttactcccg ccgcccggca ccgtagcgcg ccgtctgata gaagatacgg tggcgatgtg
    20761 tcgggcagag tatgagtgat gatacactga ccgcctgacg cactaaggaa cagccaaaat
    20821 gaccgaactt aaaaacgatc gttatctgcg ggcgctgctg cgccagcccg ttgatgtcac
    20881 tccagtatgg atgatgcgcc aggcgggtcg ctatctaccg gaatataaag ccacgcgcgc
    20941 ccaggcgggc gattttatgt cgctgtgcaa aaacgccgag ctggcgtgcg aagtgacttt
    21001 gcaaccactg cgtcgctacc cgctggatgc ggcgatcctc ttttccgata tcctcaccgt
    21061 gccggacgcg atggggttag ggctctattt tgaagccgga gaaggtccgc gttttacctc
    21121 gccagtcacc tgcaaagccg acgtcgataa actgccaatt ccggacccgg aagatgagct
    21181 gggttacgtg atgaacgcgg tgcgtaccat tcgtcgcgaa ctgaaaggcg aagtgccgct
    21241 gattggtttt tccggcagcc cgtggacgct ggcaacctac atggtggaag gcggcagcag
    21301 caaagccttc accgtgatca aaaaaatgat gtatgccgat ccgcaggcgc tgcacgctct
    21361 actcgataaa ctggcgaaaa gcgtcacttt gtatctgaat gcgcagatta aagccggtgc
    21421 tcaggctgtg atgattttcg acacctgggg cggcgtgctt accgggcgcg attatcaaca
    21481 gttctcgctt tattacatgc ataaaattgt tgatggttta ctgcgtgaaa acgacggtcg
    21541 ccgcgtaccg gtcacgctgt ttaccaaagg cggcggacag tggctggaag ccatggcaga
    21601 aaccggttgc gatgcgctgg gcctcgactg gacaacggac atcgccgatg cgcgccgccg
    21661 tgtgggcaat aaagtcgcgt tgcagggtaa tatggatccg tcgatgctgt acgcgccgcc
    21721 tgcccgcatt gaagaagaag tagcgactat acttgcaggt ttcggtcacg gcgaaggtca
    21781 tgtctttaac cttggtcacg gcattcatca ggatgtgccg ccagaacatg ctggcgtgtt
    21841 cgtggaggca gtgcatcgac tgtctgaaca gtatcaccgc taaggagcga ttatggatct
    21901 cgcgtcatta cgcgctcaac aaatcgaact ggcttcttct gtgatccgcg aggatcgact
    21961 cgataaggat ccgccggatc tgatcgccgg agccgatgtc gggtttgagc agggcggaga
    22021 agtgacgcga gcggcgatgg tgctgctgaa atacccctcg cttgagctgg tggagtataa
    22081 agtggcccgc atcgccacca ccatgcctta cattccaggt tttctttcct tccgcgaata
    22141 tcctgcgctc ctggcagcgt gggagatgct gtcgcaaaag ccggatttag tgtttgtcga
    22201 tggtcatggg atctcgcatc ctcgccgtct tggcgtcgcc agccattttg gtttgatggt
    22261 ggatgtgcca acgatcggcg tggcgaaaaa acggctctgc ggtaaattcg aaccgctctc
    22321 cagcgaaccg ggcgcgctgg ccccactgat ggataaaggc gagcagctgg cctgggtctg
    22381 gcgcagcaaa gcgcgctgta acccgttgtt tatcgctacc ggccatcggg tcagcgtgga
    22441 cagcgcgctg gcgtgggtac aacgctgcat gaaaggctat cgtctgccgg agccaacgcg
    22501 ctgggcggac gcggtggcct cggaacgtcc ggcgttcgtg cgctatacag caaatcagcc
    22561 ctaattcagg taaactgcgg ataatttccg tatttgagaa ctcaacatgt tacaaaaccc
    22621 aattcatctg cgtctggagc gcctggaaag ctggcagcat gtcactttca tggcttgctt
    22681 atgcgaacgc atgtacccca attacgccat gttctgccag caaaccggtt ttggtgatgg
    22741 gcaaatttac cgtcgtattc tcgatctcat ctgggaaacg ctgaccgtta aagacgcaaa
    22801 agtaaatttc gacagccaac tggagaaatt tgaagaagcg attccttcag ccgacgattt
    22861 cgatctgtac ggcgtttatc cggcaatcga tgcctgcgtg gcgttaagtg aactggtcca
    22921 ttcgcgtttg agtggtgaaa cgctcgaaca cgcggtggaa gtgagtaaga cctccatcac
    22981 gaccgttgcg atgctggaaa tgactcaggc tggtcgcgaa atgagcgatg aagagcttaa
    23041 agaaaaccca gctgtagagc aagaatggga cattcagtgg gaaatattcc gacttttagc
    23101 tgagtgcgaa gaacgcgata tcgagctgat aaaaggcctt agggcagacc tgcgtgaggc
    23161 gggtgagagc aatattggta taatttttca gcaataagac cagaaaacgt gatttaacgc
    23221 ctgatttgtc gtacctggag tcttcccttt cgccccccgt ctggtctaca tttggggggc
    23281 gaaaaaaagt ggctatcggt gcgtgtatgc aggagagtgc tattctggca tttccgtcgc
    23341 actcgatgct tagcaagcga taaacacatt gtaaggataa cttatgaaca agactcaact
    23401 gattgatgta attgcagaga aagcagaact gtccaaaacc caggctaaag ctgctctgga
    23461 gtccactctg gctgcaatta ctgagtctct gaaagaaggc gatgctgtac aactggttgg
    23521 tttcggtacc ttcaaagtga accaccgcgc tgagcgtact ggccgcaacc cgcagaccgg
    23581 taaagaaatc aaaattgccg cagctaacgt accggcattt gtttctggca aggcactgaa
    23641 agacgcagtt aagtaagatt gcgtggcagt gaacagtttt aacgaagggg tggtttcacc
    23701 ccttttgtct ttctggcgtc gatcattgat gctggcaggt gcgctgtttc tcactgcctg
    23761 tagccacaac tcttcacttc ctccctttac cgccagtgga tttgctgaag accagggcgc
    23821 ggtacgcatc tggcgaaaag acagcggcga taatgtgcat ctgcttgccg tgtttagccc
    23881 gtggcgcagt ggcgatacca cgacgcgaga gtatcgctgg cagggcgata acctcacgct
    23941 catcaatatc aatgtttaca gcaaaccgcc ggtgaatatt cgcgcgcgtt ttgacgatcg
    24001 cggtgatctg agctttatgc aacgtgaatc cgatggggaa aagcagcagc tttctaacga
    24061 ccaaatcgat ttataccgtt atcgtgctga tcagatccgc cagattagcg atgccttacg
    24121 tcaggggaga gtcgtgctgc gccaggggcg ctggcatgcg atggaacaga ccgtgaccac
    24181 ctgcgaaggg caaaccatta aacctgattt agattcgcag gcgatagcgc atatcgagcg
    24241 ccgccagagc cgctcttctg ttgatgtcag cgtggcatgg ctggaagcgc ccgaaggttc
    24301 gcaattactg ttagtggcaa actctaattt ctgtcgctgg caacccaacg agaaaacgtt
    24361 ctgatttacc agtggcccat acccatatga ccgccaccac cgcagccgcc gtagcccatg
    24421 cccattccgg ctccgcgcgg aatacccgct tcagccatcg cgatatctcg tttcacccgt
    24481 aactcatcta acgactgacg caaattctcc atctctttgg cgaccgcgtt aattttgctg
    24541 ctatccggtg ggttcgcggc taacagggca ttgtattcat aacgcttcgt caccagttgc
    24601 tgttgcagtg cgctgctttg agcgtaaaag tcattatgga ttttctgcca cgctgtctgt
    24661 tgttcgctgg tcaaaggcgc ggcattttgc tgccacatac cgtgtccgcc gtgagcaaat
    24721 gcagatgtcg atcccatcgc cattgctgaa agcgccatca ttaccagggc aattttcgtg
    24781 ttccgtttca tggttaatcc tccagtggtt gtcttacttc gggtattgca tcttccgtgc
    24841 caacgatgaa acgctgatat gacgggtaat ctggcatgat aaacgagtaa aaatgactcg
    24901 cctgctgcgg atagcgagtc atttttactc attgaaactt aagcctttgt gttacagcgc
    24961 agggtaagcg ctgataaaag atggcatgat ttctgctgtc agaaagggat gagcaggcaa
    25021 agaagaagat gcgttttatg caacgttcta aagactcctt agctaaatgg ttaagcgcga
    25081 tcctccccgt ggtcattgtt gggctggtag ggttgtttgc ggtgacggtg attcgcgatt
    25141 atgggcgcga gactgccgcc gccagacaaa cgctgctgga aaaaggcagt gtacttattc
    25201 gcgctcttga atccggctcg cgcgtcggca tggggatgcg catgcatcat gcgcagcagc
    25261 aggccttact ggaagaaatg gccgggcagc ctggagtacg ttggtttgcg gtcacggatg
    25321 aacaaggaac aatcgtgatg catagcaact ccggcatggt gggaaaacag ctttattccc
    25381 ctcaggaaat gcagcagtta catccgggag atgaagaagc gtggcggcgg atcgatagcg
    25441 cagacggcga gcctgttctg gaaatttatc gccagtttca accgatgttt gctgctggaa
    25501 tgcaccggat gcgccatatg cagcagtatg ccgcgacacc acaagcaatt ttcatcgctt
    25561 tcgacgccag taatattgtg agtgccgaag atcgtgagca gagaaacacc ctgattattc
    25621 tcttcgccct ggcgacggtc ttgctggcaa gcgtattgtc attcttctgg tatcgccgct
    25681 atctgcgctc gcgccagctt ctacaagatg aaatgaagcg caaagagagg ctggtggcgc
    25741 tggggcatct tgcggcaggc gttgcccacg aaatccgtaa cccactttcc tcgattaaag
    25801 gactggcgaa atactttgcc gagcgcgcgc ctgcaggggg agaagcgcat caactggcgc
    25861 aggtgatggc gaaagaggcc gaccgtttaa accgcgtggt aagcgagttg ctggaactgg
    25921 ttaagccaac gcatctggct ttgcaggcgg tggatctcaa cacgctgatt aaccactcat
    25981 tacagctggt aagccaggat gcaaacagcc gggagatcca gttacgcttt accgccaacg
    26041 acacattacc ggaaattcag gccgatccgg acaggctgac tcaggtcctg ttgaatctct
    26101 atctcaatgc tattcaggcg attggtcagc atggcgtgat tagcgtgacg gtcagcgaaa
    26161 gcggcgcggg cgtgaaaatc agcgttaccg acagcggtaa gggaattgcg gcagatcagc
    26221 ttgaagccat cttcactccg tacttcacca ccaaagccga aggcaccgga ttggggctgg
    26281 cggtcgtgca taatattgtt gaacaacacg gtggtacaat tcaggtcgca agccaggagg
    26341 gaaaaggctc aacgttcacc ctctggcttc cggtcaatat tacgcgtaag gacccacaag
    26401 gatgacgcac gataatatcg atattctggt ggtggatgat gacattagcc actgcactat
    26461 tttgcaggct ttactgcgcg gctggggcta taacgtcgcg ctggcgaaca gcgggcgaca
    26521 ggcgctggag caggtgcggg aacaggtttt tgatcttgtg ctttgcgatg tgcgaatggc
    26581 ggagatggac ggcatcgcca cgctgaaaga gatcaaagcg ttaaacccgg caattccggt
    26641 gctgattatg actgcgtact ccagcgtcga gacggcggta gaggcactga aaactggggc
    26701 gctggattat ctcatcaagc cgctggattt cgataaccta caggcgacgc tggaaaaagc
    26761 gctcgcgcat acgcacagta ttgatgctga aacacctgcg gtgactgcca gccagttcgg
    26821 tatggtcggt aaaagcccgg cgatgcaaca cctgctcagt gaaatcgccc tcgtcgcgcc
    26881 atcggaagcc acggtactga tccacggcga ttccggcact ggtaaagagc tggtcgccag
    26941 ggcgattcac gccagtagcg cacgtagcga aaaaccgctg gtaacgctca actgtgcggc
    27001 actcaacgaa tccttgctgg aatctgaatt gttcggtcac gaaaaagggg cgtttactgg
    27061 agccgataaa cgccgggaag ggcgctttgt tgaggcggac ggcggcacgc tgtttctcga
    27121 tgaaattggc gatatctcgc cgatgatgca ggtgcgtctg ctgcgtgcga ttcaggagcg
    27181 cgaagttcag cgtgtcggta gcaaccagac tatctcggtt gatgtccggc tgattgcggc
    27241 gacccatcgc gatcttgccg cagaggtgaa tgccgggcgt tttcgccagg atctctacta
    27301 tcgcctgaat gtggtggcga ttgaagtacc atcgctgcgg caacggcggg aagatatccc
    27361 tctgctggct ggccattttc tgcagcgctt tgccgagcgt aatcgcaagg cggtaaaagg
    27421 ttttacgccc caggcgatgg atctgttgat tcattacgac tggccgggaa atattcgtga
    27481 gctggaaaac gcggtggaac gggcagtggt gctgctgacc ggggaatata tttccgaacg
    27541 cgagctgccg ctggcgattg ccagtacgcc gatcccgctg ggacaaagtc aggatattca
    27601 gccgttggtg gaagtggaaa aagaggtgat tctggcggcg ctggagaaaa cgggcggcaa
    27661 caaaaccgaa gccgcccgtc agttagggat cacgcgcaaa acgctattgg caaaactgtc
    27721 gcgttagttc tgctcgcgtt cgatagcgcg ccagccgata tctttccggc agaagcagtc
    27781 gtcccagtga atatcggtca ttaaggcata agcgcgtttc tgcgcttctg ccacggtatg
    27841 acccagcgcg gtgacgcaca gtacgcgccc gccgctggtc actacctgct cgtcatccgc
    27901 cagttttgtg cctgcatgga acactttgcc gtcttccact tcttccagcg gcaggccgtg
    27961 gatcacatca ccggtgcggt aatcgcccgg atatccaccc gcagccatca ccacgccgag
    28021 agaagcgcgt tcatcccact cggatgtttt ctcgtccagc ttcccttcac aggctgccag
    28081 gcagagctca accagatcgg acttcatgcg cagcataatc ggctgggttt ccggatcgcc
    28141 aaagcggcag ttaaattcga taaccttcgg attgccctgt ttgtcgatca tcaggcccgc
    28201 gtagagaaaa ccggtgtagg tgttgccttc cgccgccatg cctttcacgg ttggccagat
    28261 gatacgttcc atggtgcgct gatgaacttc atcggttact accggcgcgg gggagtaagc
    28321 gcccatcccg cccgtgtttg gtccggtatc tttatcgcct acgcgtttgt gatcctggct
    28381 ggtggccatc ggcaacacat gctcgccgtc caccatcacg ataaagctcg cttcttcgcc
    28441 atcgaggaac tcttcgataa cgatgcgatg acccgcgtcg ccaaaagcgt tgcccgccag
    28501 catatcgtga acagccgctt ccgcttcttc cagcgtcatc gccacgataa cgcctttccc
    28561 ggcagccaga ccgtccgctt taatgacgat tggcgcgcct ttctcacgca gatacgccag
    28621 cgcaggttct acctcggtga agttctggta ttccgccgta gggatcttat ggcgggccag
    28681 gaaatctttg gtaaacgctt ttgagccttc cagttgggcc gcacctgcgg ttgggccgaa
    28741 gattttcagc ccggcggcgc ggaaggtatc gaccacgcct ttcaccagcg gcgcttccgg
    28801 gccgacgatg gtcagatcaa tcttttcgtt ttgtgcgaaa tccaacagcg ccgggatatc
    28861 ggtcacgcca atagcaacgt tttgcagcgc gggttccagc gcagtgcctg cattacccgg
    28921 agcaacaaaa acagtctcaa ccagcggcga ctgggccgct ttccaggcca gcgcgtgctc
    28981 gcgcccgccg ttaccaatca ctaatacttt catctattgc tccattaatg gcggaagtgg
    29041 cgcatgtcgg tgaagagcat cgcaataccg tgctcgtcgg cggcggcaat cacttcgtca
    29101 tcacggatgg aaccgccagg ctggattacg caggtcacgc ccgcagcggc ggcggcatca
    29161 ataccgtcgc ggaacgggaa gaacgcgtca gaagccatcg aggaaccttt cacttccagg
    29221 ccttcatcag cagctttaat accggcgatt ttcgcggagt acacgcggct catctggccc
    29281 gcgccaatac cgatggtcat attgtttttg gcatagacga tagcattgga tttcacgaac
    29341 ttcgccactt tccagcagaa cagcgcatcg cgcagttcct gttcggtcgg ctgacgttgg
    29401 gtgacgacgc gcagttcttc cgcaccgacc atccccaggt cacgatcctg aaccagcaga
    29461 ccgccgttca cgcgtttgaa atcaagaccc ggaacacgct cgccccactg accgcaggtc
    29521 agaacgcgta cgttctgctt ggcggcggtg attttcaggg cttcttcgct ggcggaaggc
    29581 gcaataatca cttcgacaaa ctgacgagaa atgatggcct gtgcggtttc cgcatccagc
    29641 tcgcggttaa aggcaatgat gccgccgaat gcggaggttg ggtcggtttt gtacgcgcga
    29701 tcgtaagcat caagaatgga attgccgata gctacaccgc aagggttggc gtgcttcaca
    29761 atcacacatg ccggctcggc gaactctttc acgcactcca gcgccgcatc ggtatcggcg
    29821 atgttgttat aagagagggc tttaccctga acctgggttg cggtagcaac ggaggcttct
    29881 ttcacattct cttctatata gaaggcagcc tgctggtggc tgttctcgcc gtaacgcata
    29941 tcctgcttct taatgaagtt caggttcagc gtgcgtggga agcgaccggc ggcttctttg
    30001 ctttcaccgt ggtacgccgg aaccatgctg ccgaagtagt tggcaatcat gctgtcgtag
    30061 gcggcagtgt gttcgaaggc tttgatggcg aggtcgaaac gggttgcaag cgtcagcgat
    30121 ccttcgttgt catccatctc tttaataatg gcgtcgtagt cgctgctctt caccacgatt
    30181 gcgacatctt tatggttctt agcggcggag cgcaccatgg tcgggccgcc gatatcgatg
    30241 ttctcaaccg catcttccag cgagcaacct tcacgggcca cggtctgggc gaacggatac
    30301 aggttaacaa ccaccatatc gataggctgg atctgatgtt cttccataat ggcatcgtcc
    30361 tggccgcgac ggcccagaat gccaccatgt actttcggat gcagggtctt cacgcgtcca
    30421 tccatcatct ccgggaaacc ggtgtaatcg gaaacttcgg ttaccggcag acctttttct
    30481 gctaacagac gggcagtgcc ccctgtagac agcagctcca caccgcgtgc ggaaagtgcc
    30541 tgggcgaatt cgacgatacc ggctttgtca gaaacactga gcagagcgcg gcggactgga
    30601 cgacgttgtt gcatggtaaa tcccctggat ttgactatta cagagagcgt tagctgaatt
    30661 tttcgcgaaa aactcagcta acgcccctaa cggggcatcc ttatttttcg cccgcattgt
    30721 aacgaaaacg tttgcgcaac gctcgcgaat ttttctcttt caatggtgat cacaattttg
    30781 acagtggtta ccgtgggaaa aatacagaaa ttacattgat gattgtggat aactctgtgc
    30841 gtaaaaaggt ataaagcggg gttttgctgg ggaatgcagc agtcagtcat ttttctgcaa
    30901 tttttctatt gcggcctgcg gagaactccc tataatgcgc caccactgac acggaacaac
    30961 ggcaaacacg ccgccgggtc agcggggttc tcctgagaat ctcaacagag aaaagcaaa
//
LOCUS       DAZKIV010000039        30433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZKIV010000039 DAZKIV010000000
VERSION     DAZKIV010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.039"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2448
                     /locus_tag="ACHOT6_001788"
     CDS             <1..2448
                     /locus_tag="ACHOT6_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9947020.1"
                     /translation="SLSVLIIGCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVG
                     KYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGC
                     YPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYAS
                     HYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRP
                     IPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKV
                     TVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTR
                     KGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAG
                     VGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDK
                     NFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNY
                     SLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQD
                     NKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQR
                     DSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGI
                     GVIPSVSSYITTSLSVDTRNLPENVDIENSVIITTLTEGAIGYAKLDTRKGYQIMGVI
                     RLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPN
                     SSNLTTGTVILPCISQN"
     gene            2464..3213
                     /gene="yqiH"
                     /locus_tag="ACHOT6_001789"
     CDS             2464..3213
                     /gene="yqiH"
                     /locus_tag="ACHOT6_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9947021.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3215..4279
                     /gene="yqiI"
                     /locus_tag="ACHOT6_001790"
     CDS             3215..4279
                     /gene="yqiI"
                     /locus_tag="ACHOT6_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9947022.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTTVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4322..4522)
                     /gene="glgS"
                     /locus_tag="ACHOT6_001791"
     CDS             complement(4322..4522)
                     /gene="glgS"
                     /locus_tag="ACHOT6_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFP9947023.1"
                     /translation="MDHRLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            4791..5420
                     /gene="yqiJ"
                     /locus_tag="ACHOT6_001792"
     CDS             4791..5420
                     /gene="yqiJ"
                     /locus_tag="ACHOT6_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFP9947024.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLDIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            5447..7108
                     /gene="yqiK"
                     /locus_tag="ACHOT6_001793"
     CDS             5447..7108
                     /gene="yqiK"
                     /locus_tag="ACHOT6_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFP9947025.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIDREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGGAAGDASAGSVSGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSSTPVEEKAE"
     gene            7346..7405
                     /locus_tag="ACHOT6_001794"
     CDS             7346..7405
                     /locus_tag="ACHOT6_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFP9947026.1"
                     /translation="MMKLVIILVVLLLVSFAAY"
     gene            complement(7525..8958)
                     /gene="hldE"
                     /locus_tag="ACHOT6_001795"
     CDS             complement(7525..8958)
                     /gene="hldE"
                     /locus_tag="ACHOT6_001795"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFP9947027.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGMVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(9006..11846)
                     /gene="glnE"
                     /locus_tag="ACHOT6_001796"
     CDS             complement(9006..11846)
                     /gene="glnE"
                     /locus_tag="ACHOT6_001796"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFP9947028.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(11869..13170)
                     /gene="ygiF"
                     /locus_tag="ACHOT6_001797"
     CDS             complement(11869..13170)
                     /gene="ygiF"
                     /locus_tag="ACHOT6_001797"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFP9947029.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            13412..14032
                     /gene="ygiM"
                     /locus_tag="ACHOT6_001798"
     CDS             13412..14032
                     /gene="ygiM"
                     /locus_tag="ACHOT6_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFP9947030.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            14096..15334
                     /gene="cca"
                     /locus_tag="ACHOT6_001799"
     CDS             14096..15334
                     /gene="cca"
                     /locus_tag="ACHOT6_001799"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFP9947031.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(15497..16318)
                     /gene="bacA"
                     /locus_tag="ACHOT6_001800"
     CDS             complement(15497..16318)
                     /gene="bacA"
                     /locus_tag="ACHOT6_001800"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9947032.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(16409..16777)
                     /gene="folB"
                     /locus_tag="ACHOT6_001801"
     CDS             complement(16409..16777)
                     /gene="folB"
                     /locus_tag="ACHOT6_001801"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFP9947033.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEIAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     VRAANVGVIIERGNNLKENN"
     gene            16882..17499
                     /gene="plsY"
                     /locus_tag="ACHOT6_001802"
     CDS             16882..17499
                     /gene="plsY"
                     /locus_tag="ACHOT6_001802"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFP9947034.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(17512..18444)
                     /gene="ttdR"
                     /locus_tag="ACHOT6_001803"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(17512..18444)
                     /gene="ttdR"
                     /locus_tag="ACHOT6_001803"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
     gene            18651..19562
                     /gene="ttdA"
                     /locus_tag="ACHOT6_001804"
     CDS             18651..19562
                     /gene="ttdA"
                     /locus_tag="ACHOT6_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFP9947035.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAK
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            19559..20164
                     /gene="ttdB"
                     /locus_tag="ACHOT6_001805"
     CDS             19559..20164
                     /gene="ttdB"
                     /locus_tag="ACHOT6_001805"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFP9947036.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GRMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            20213..21676
                     /gene="ttdT"
                     /locus_tag="ACHOT6_001806"
     CDS             20213..21676
                     /gene="ttdT"
                     /locus_tag="ACHOT6_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFP9947037.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(21719..22732)
                     /gene="tsaD"
                     /locus_tag="ACHOT6_001807"
     CDS             complement(21719..22732)
                     /gene="tsaD"
                     /locus_tag="ACHOT6_001807"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFP9947038.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            22969..23184
                     /gene="rpsU"
                     /locus_tag="ACHOT6_001808"
     CDS             22969..23184
                     /gene="rpsU"
                     /locus_tag="ACHOT6_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFP9947039.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            23295..25040
                     /gene="dnaG"
                     /locus_tag="ACHOT6_001809"
     CDS             23295..25040
                     /gene="dnaG"
                     /locus_tag="ACHOT6_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFP9947040.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            25235..27076
                     /gene="rpoD"
                     /locus_tag="ACHOT6_001810"
     CDS             25235..27076
                     /gene="rpoD"
                     /locus_tag="ACHOT6_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFP9947041.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(27154..27660)
                     /gene="mug"
                     /locus_tag="ACHOT6_001811"
     CDS             complement(27154..27660)
                     /gene="mug"
                     /locus_tag="ACHOT6_001811"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFP9947042.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKRAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            27785..27860
                     /locus_tag="ACHOT6_001812"
     tRNA            27785..27860
                     /locus_tag="ACHOT6_001812"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27818..27820,aa:Ile,seq:cat)
     gene            complement(27948..28793)
                     /locus_tag="ACHOT6_001813"
     CDS             complement(27948..28793)
                     /locus_tag="ACHOT6_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9947043.1"
                     /translation="MSAITVSRPEVVNGHTDVICSTSVSHILAVRKSSLLQIDTLIRQ
                     LVEISVLTESIGGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANIWSTFEQLHQNKDEVFERGVINVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADGLADLERMLHLFSGKPLPDNR
                     ENITLRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     QLSAI"
     gene            complement(28878..29075)
                     /locus_tag="ACHOT6_001814"
     CDS             complement(28878..29075)
                     /locus_tag="ACHOT6_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9947044.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRILRQLQFLQQKR"
     gene            complement(29095..29583)
                     /locus_tag="ACHOT6_001815"
     CDS             complement(29095..29583)
                     /locus_tag="ACHOT6_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9947045.1"
                     /translation="MNLSLTLEADSVNVQALNMGRIVVDVDGVNLAELINVACDNGYS
                     LRIVDESDRTSAERTPPFAALTGIRCSTAHITAKDNVWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTGAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGFSLIHLDASAECLPGLPTF
                     DW"
     gene            complement(29580..29960)
                     /locus_tag="ACHOT6_001816"
     CDS             complement(29580..29960)
                     /locus_tag="ACHOT6_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFP9947046.1"
                     /translation="MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFS
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGVPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGRHPEEAKQ"
     gene            complement(30049..30417)
                     /locus_tag="ACHOT6_001817"
     CDS             complement(30049..30417)
                     /locus_tag="ACHOT6_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9947047.1"
                     /translation="MSDTLHETNYPNDNHNRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     DDRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVALYAKGLTCDAD
                     TLGSCGYVYLAVYPTPDMKK"
BASE COUNT         7640 a         7983 c         7503 g         7307 t
ORIGIN      
        1 tcattatccg ttctcatcat tggttgtgcg tcagcttatg ccgttgaatt caacaaagat
       61 ttaatcgaag ccgaagatcg tgaaaacgtt aacctttccc aatttgaaac tgatggccaa
      121 ttacccgtag gtaaatactc attaaacgct cttattaata ataaaaggac gccaatccat
      181 ctggacctgc aatgggtatt aattgataac caaactgcag tttgtctgac tccagagcaa
      241 ttaacattat taggctttac tgatgagatt attgaagtgg ctcagcaaaa cctgatcgat
      301 ggttgttacc ctatcgaaaa agaaaaacaa attacaactt atctcgataa agggaaaatg
      361 caattatcca tatctgcacc tcaggcatgg ttaaaataca aagatgcaaa ctggacgcct
      421 cctgaacttt gggatcatgg tattgctggg gcatttcttg actacaattt atatgcctct
      481 cattatgcac cacatcaggg cgataattca caaaatataa gttcctatgg gcaggctggg
      541 gttaatcttg gggcctggcg cctgcgtact gattaccagt acgatcagtc atttaacaat
      601 ggcaaaagcc aggcgaacaa cctggatttt ccgcgtattt atttgtttcg cccaatccca
      661 gcaattaatg caaaactaac tataggtcaa tacgatactg aatcctctat tttcgactct
      721 ttccattttt ctggcgtttc gttgaaaagc gatgagaata tgttgccgcc tgatctacgt
      781 ggttacgcac cgcaaatcac gggtgttgca caaacgaatg caaaggtcac tgtctcacag
      841 aacaaccgta ttatttatca agaaaatgtt cctcctggcc catttgctat tactaattta
      901 ttcaatacat tacaggggca actggacgtc aaggttgaag aagaggacgg gcaagtcacg
      961 caatggcaag ttgcatctaa tagtattcct tatctgacgc gtaaagggca gattcgctac
     1021 accactgcta tgggtaaacc gaccagcgtt ggtggtgatt ccttacaaca acccttcttc
     1081 tggactggtg aattctcatg gggttggctg aacaatgtat ccctgtatgg tggttcagtt
     1141 ttaacaaacc gtgattatca atctctggct gccggcgttg gctttaacct taactcattg
     1201 ggttcattat cttttgatgt cacacgatct gatgctcagt tgcataatca ggataaagaa
     1261 acgggttata gctaccgcgc taactattca aaacgttttg aatctaccgg tagccagctc
     1321 actttcgctg gttaccgttt ctctgataaa aactttgtga caatgaatga atatatcaat
     1381 gacactaacc attacacgaa ttatcagaat gaaaaagaga gttatattgt cacgtttaac
     1441 cagtatcttg aatcattaag gttaaataca tacgtaagtt tggctcgtaa tacttactgg
     1501 gacgccagca gtaatgtgaa ttattcatta tcacttagcc gcgattttga tatcgggcca
     1561 ttaaaaaacg tctccacttc tctaacattt agccgaataa actgggaaga agacaaccag
     1621 gatcaactgt acctaaatat ttcgattccc tggggaacca gtagaacatt gagctatggt
     1681 atgcaacgaa atcaggataa caagatttcg catactgctt cgtggtatga ctcttccgat
     1741 cgaaataatt cctggagcgt ttctgcttca ggcgacaatg atgaattcaa agatatgaaa
     1801 gcgtcactac gcgccagtta tcagcataat accgagaacg gtcgtctcta tctctccggg
     1861 acatcacagc gagacagtta ttattctctg aatgccagtt ggaatggttc attcacggcg
     1921 actcgtcacg gagccgcttt ccatgactat agtggtagtg ctgactcgcg ttttatgatc
     1981 gacgcagatg gtgcagaaga tattccgttg aacaataaac gcgcggtaac taatcggtat
     2041 ggcatcggag ttattccatc agtcagcagt tacataacaa catcattaag cgttgatacc
     2101 cgaaatcttc cagaaaatgt ggatatcgaa aactcggtta tcatcaccac cttaaccgaa
     2161 ggtgctattg gctacgccaa acttgatacc cgcaagggct atcaaatcat gggggttatt
     2221 cgcctggcag atggtagtca cccaccactg gggattagcg taaaagataa aaccagccat
     2281 aaagaattag gactggttgc tgatggcggc tttgtatacc tcaacggcat tcaggatgac
     2341 agcaaactta ctttacgctg gggtgataaa tcctgtttta ttcaaccacc caatagcagc
     2401 aacttaacca ccggaacggt tattttaccg tgtattagcc aaaattaatt caggatgaaa
     2461 ctaatgcgct acttaaacac aaagaatctc attgcagcag gagtattact cgcctgcatg
     2521 agcagcattg cctggggtgc catcatcccg gaccgtaccc gtatcataat gaatgagtca
     2581 gataaagggg aagcattaaa acttactaat caaagcaaaa atctacccta tctggcgcaa
     2641 acatggatag aagatacgaa aggcaataaa tcacgtgact ttattgtcac ggttccccct
     2701 atggtacgtt taaatcccag tgagcaaatt caaataagaa tgataagtca ggaaaaaatt
     2761 gctcaactgc ctaatgacag agaaacgctc ttctatttta acgtgcgaga aataccgcca
     2821 aaaacagata aaaaaaatgt gatgcaagtc actatgcagc acgcattgaa actattctgg
     2881 cgaccaaaag ctattgaatt agaagatgat ggggtaatgt cttacgaaaa agttgaaatc
     2941 atccgtagaa atgatggttc gatccgcttt aacaataaga tgccttatca cgttacgctg
     3001 ggttatatcg gaacaaacgg aatcacaatg ttgccacaaa cgcaaagttt aatggtaacc
     3061 ccttatagcc atgcagatac gcagtttaaa aatgttccgt cagcttttca ggttggttat
     3121 atcaatgatt ttggtggatt aagtttttat gaaataaact gcccgacggt taataactct
     3181 tgtaatgttt ccgtagccaa acgagataaa taatatgcgc tacttattaa ttgtaattat
     3241 atttattata ggttttagtg cattacctgt atgggcaatg gactgctatg ctgaacatga
     3301 agggggaaat accgtagtca ttggttacgt accaagaatt tctatcccca gtaatggtaa
     3361 aaaaggtgat aagatctggc aaagcagtga gtattttatg aatgtcttct gtaataatgc
     3421 attacctgcc ccatcacctg gagaagaata cccatcagcc tggactaata taatgatgtt
     3481 tttaccaggc ggtcaggact tttataatca gaactcctat atttttggcg taacatacaa
     3541 tggagttgat tatgattcga ctgcccctaa tgctatcgca gcaccagaat gcattgatat
     3601 aaaaggagcg ggtaccttta ataatcacta taaaaacccc gctgtctgta gtggaggtcc
     3661 tgagcctcaa ttatcagtaa cctttccagc tcgcgtacaa ctctatatta aattagcaaa
     3721 aaatgccaat agagttaata aaaacctggt gttacctgat gaatatatcg ctctagagtt
     3781 taaaggtatg agtggcgcag gagctataga agtagacaaa aatttgacct tcaggattcg
     3841 tggattaaat aatattcatg tccttgactg ctttgttaat gtcgcactgg aaccagctga
     3901 tggcgttgtt gactttggta aaataaattc ccgaacaatt aaaaatacca ccgttagtga
     3961 gacgtttagt gtagtcatga caaaagatcc tggtgcggcc tgtactgagc aattcaatat
     4021 tttagggagc tttttcacta cggatatttt gagtgattat agccatctgg atatgggtaa
     4081 tggactgcta ttgaagatat ttcataaaga tggaacagca acggaattta accgcttctc
     4141 acaatttgct tctttttcat cgtctagtgc accttcggtc accgcaccat tcagggcaga
     4201 acttagtgcg aatccggcag agactgttgt tgagggaccg tttagtaaag acgtcatcct
     4261 caaaatcacc tataactagc atctaataca aacactaaaa cgggccatca ggcccgttat
     4321 atcagtgctc taactccagc tctcttgcct gcatcatctg ttgacaatac caggcataac
     4381 gtgcgcaaaa ccaggttcta tgttcttcat ccatgttacc agtaacggca agaatatcga
     4441 ccgggcgacc ttctgcgtgc attctggcaa aactacgcgc caggaaatcg aaattattta
     4501 aagaattaag tctatgatcc ataagcacat cctccggtta tcgcatacct tttaaggcca
     4561 tatgcttttt ctgatttcag tattccccaa tcagtgtagt gcctttaaac ataacgtgcg
     4621 taaatatatt gtccccgatc acacttttta gtgaaaaata aaaaaccata ttaaaaaact
     4681 ttctcttcca gaacttctct aatatgtgtt attattaaca aagataaatg taagcaagga
     4741 aactatccca aagtaaatat tgcgtcacta aatggacatt ggagtgacat atgattttat
     4801 tcgccgacta taatacccct tatcttttcg ctatttcctt tgtactgttg atcggccttt
     4861 tagaaatttt agcgcttatc tgcggtcaca tgctctccgg tgctcttgat gcacatcttg
     4921 accattatga ttccataacc acaggtcata ttagccaggc acttcattat ctcgatatcg
     4981 gaagattgcc agctctcgtc gttctctgct tgctggctgg tttttttggt cttattggca
     5041 tcctgttgca gcatgcctgc atcatggtct ggcaatcacc actgtcgaac ctgttcgtgg
     5101 ttcctgtcag tttactgttt acgattattg cggtgcatta taccggtaaa atagttgcgc
     5161 cctggattcc acgcgaccat agttcagcta ttacagaaga agaatatatt ggtagcatgg
     5221 cattaattac cggtcatcaa gccacttcag gtaatccctg cgaaggaaaa ctcaccgatc
     5281 aatttggcca aatacattat ttactactgg agcctgaaga aggaaaattt ttcactaaag
     5341 gagacaaagt gttaattatt tgccgacttt ctgcaacgcg atatttggcg gaaaacaatc
     5401 cctggccaca aatattgtaa tttattaatc aaaaggaaat ttaataatgg atgatattgt
     5461 taattctgtg ccctcctgga tgtttaccgc gattattgcc gtatgcattc tgtttattat
     5521 tggaattatt ttcgccaggc tctatcgtcg cgcttcggca gagcaagcat ttgtccgtac
     5581 cggcttaggt gggcaaaaag tggtgatgag cggcggcgca atcgtgatgc caatcttcca
     5641 tgaaattatc cctatcaata tgaataccct gaaactggaa gtcagtcgct cgacgataga
     5701 cagcctcata accaaagatc gtatgcgtgt tgatgtagtc gttgctttct ttgtgcgggt
     5761 aaaaccttca gtagaaggga ttgccaccgc tgcccagacg ctggggcaac gcaccctgtc
     5821 gcctgaagac ttacgtatgt tggttgaaga taaatttgtc gatgccctgc gtgcaacagc
     5881 tgcgcaaatg accatgcatg agttacagga tacccgcgag aacttcgtgc agggggtaca
     5941 aaataccgta gctgaagatc tgtcgaaaaa tggcctggaa ctggaaagcg tttcacttac
     6001 caactttaac cagacctcga aagaacattt caatccgaac aatgcctttg acgccgaagg
     6061 tttaaccaaa ctgactcagg agacggagcg ccgtcgtcgc gaacgtaacg aagttgaaca
     6121 ggatgtagaa gttgcggtgc gtgagaaaaa ccgtgatgca ctttcgcgca agttggagat
     6181 tgaacaacaa gaagcgttta tgacgcttga gcaggagcag caggttaaaa cccgtactgc
     6241 tgaacagaat gctcgtattg cggcttttga agctgaacgt cgccgcgaag cagagcagac
     6301 gcgaattctg gctgaacgac agattcagga aacagaaatc gaccgcgaac aagccgtccg
     6361 ctcaagaaag gttgaagccg aacgtgaagt tcgcattaaa gagatcgaac aacagcaggt
     6421 caccgaaatc gccaaccaga cgaaatcgat cgctattgcc gccaaatcgg aacaacagtc
     6481 ccaggcagaa gcgcgtgcta accttgcact cgcagaagca gtaagcgccc aacaaaacgt
     6541 tgaaaccact cgccagaccg cagaagccga tcgagctaaa caagttgccc taatcgctgc
     6601 cgcgcaggat gcagaaacca aagcggttga actgaccgtg cgggcgaaag cagaaaaaga
     6661 agccgcagag atgcaggcgg cggctatcgt tgagttagcc gaagctacac gtaaaaaggg
     6721 tctggcggaa gcagaagcac aacgtgcact gaacgatgct atcaacgtac tttctgatga
     6781 acaaaccagc cttaaattca aactggcatt attgcaggcg ctgcctgcgg taattgagaa
     6841 atccgtggaa ccaatgaagt ctatcgacgg catcaaaatt attcaggttg atgggctgaa
     6901 tcgtggcggc gctgcgggtg atgcgagcgc aggtagcgtt agtggaggaa accttgcaga
     6961 gcaggcattg tctgccgcac tttcttaccg cacacaggca ccgctgattg actccttgct
     7021 caatgaaatt ggcgtttcag gcggctcact ggcggcattg acttcaccct taacctcatc
     7081 aactcccgtc gaagaaaaag cagagtaaac cctcttctat gaataatccc tggcccaaaa
     7141 gccggggatt atttatatct gcgttccgct aaaaggtgca aatgatcagg ctattgcaga
     7201 attttgcgtg acggctcggg gaaggaaaaa ctggctctgg gaaagcattg cgcggggtct
     7261 gacgctcatc aacaatcggg gagtcagcag gggctgaaac gggaaaggcc ctcccgaaga
     7321 aggggcctta tagaaggaaa gggttatgat gaagctcgtc atcatactgg ttgtgttgtt
     7381 actcgtaagt ttcgcagctt attaacagcc aatcagaggg gggagaaatc ctccctcacc
     7441 cttgttcctt tactctaggt tgaaaaaaca acagcgtcaa taggcctgcc atgtacgaag
     7501 cgagatctgt gaaccgcttt ccggttagcc ttttttatcc tgttggatct tcttgataat
     7561 gttagtggtc gagcaaccgt cttcaaagtt gagcaccaac acttcgccgc cgttggccca
     7621 gacttcttta ctcccggcaa tctcttctgg tttataatca ccgcctttca ccagcagatc
     7681 tggcaggatc ccggcgatca agcgctgcgg cgtgtcttct tcaaacgaca ccacccagtc
     7741 gaccgcttcc agtgcgccca gcacaatcat acgctgttcg agcgggttta cggggcggga
     7801 atcccctttc agccgtttgg tggaggcatc gctgttcaca gcgacaatca gacggtcacc
     7861 cagtttgcga gcatttgcca gataggagac gtgcccggcg tgcaggatgt caaagacacc
     7921 gttggtcatc accacttttt caccgcgttt acgcgccgcc gctacagcaa gcttcagttc
     7981 ctcttcggtc atcacaccaa agccggtatc tgcgcgccca cgcactgcat tttccagctc
     8041 gatcggcgaa accgtggagg ttcccagttt gccgaccacc atgcctgccg ccgcattggc
     8101 aaagaagcag gcttcttcca gcgaattacc cgctgccagc gttgccgcca ggacgccaat
     8161 caccgtgtcg cccgcgccgg taacgtcata cacttcctgc gcctgggttg gcatatgcag
     8221 cggcgcttta cccggttgca gcagcgacat accctgttcg gaacgggtca ctaacagagc
     8281 cgagagttcg taatcggcaa tcagtttcat gccgcgctca acaatctctt cttcggtctt
     8341 acatttaccg acaacagctt caaattccga gagattcggc gttaacagcg tagcgccgcg
     8401 gtagcgctca aaatcggtac cttttggatc aatcagcacc ggaacgcccg ctttacgcgc
     8461 cagttggatc atctgctgta cgcttgccag agcacctttg gcgtagtcag aaagcaccag
     8521 cgcgccaatc gaactcagcg cctgattaat ccgctcgtgc agcggctgcg gatcaacacc
     8581 ttcgaaacct tcttcaaaat ccaggcggat cagctgttgg ttgcgggaaa gtacccgtaa
     8641 tttggtgata gtcggatgcg tcggtacaga aacgaagtcg catttgacgt tgacgtcggc
     8701 cagagattta ctcagcgcac gcgccgcatc gtcaatgccc gtcagcccga ccaggcgagc
     8761 attggcaccg agagaagcga tgttcattgc cacgttagcc gcgccgcccg gacgttcttc
     8821 gatggtattg actttaacca cgggcaccgg cgcttccggc gagatacggc tggtgggacc
     8881 gtaccagtaa cgatccagca tcacatcacc aaccaccatc actcctgcac gttcaaactc
     8941 tggcagcgtt actttcattc ctgtctcctg agagattcaa aatttgcgcg cgataatacc
     9001 atacttcatt cttccaccag ccacttctgc cagcttgccc gcaccagttc acgctctgcg
     9061 gtgaagcaat cctccggcac atggcccggc aattcctgta atgccagatg atgaagttca
     9121 tcgcgcagcg tagtataagc acgggtcagc gccatcgctt cctgctcttc cataatgtcg
     9181 ttttgcgcca gtagttccag aatgcgcacg ttgtctgacc agcgcgttaa cttcggtttt
     9241 tcatgagcgt agcgcaacac cagatattgg gtaataaatt cgatatcggt aattccgcct
     9301 tcatcagctt tgatatcaaa gcgatcgcga tgtttattgc cgagatgagc gcgcattttc
     9361 tcgcgcattt cccgcacttc cgtttgcaga gttttacctt cacgcggcag cgtcataatc
     9421 tcgcgacgca ccgcgtcaaa gtgcgcggtg agctgcggat cgccgtacac tacacgcgca
     9481 cgcaccagcg cctgatgctc ccacgtccag gcctcgtttt tctgataatc agcaaatgct
     9541 tctgcggatg tcaccagcat tcccgccgcc ccggacggac gcagtcgtgc atccacttca
     9601 tacaaaatgc cggaagaggt tcgcgtactg aacagatgca taatgcgttg cgccaaacgc
     9661 agataaaact gtcgcccgtc gatttcccgc tcgccgtcag tcatcgcatc cattgggcaa
     9721 tcatggagga agatcaggtc aagatcggaa ctgtagccta actcccagcc gcccagcttg
     9781 ccgtagccga ccaccgcaaa accacggcct tcgcgatcgt tgaggtgatt tggcttaccg
     9841 tagcgggcca ccatttgaac ccacgcctgc tgaacgacgg catctatcat agcctctgcc
     9901 agccaggtta agtgatcgct cactttcatc accggtagcg taccagcgat atccgctgcg
     9961 gcgatgcgta acagctgcgc ctgtttgaac tgacgcagcg cctcaagctg ttgctcttcg
    10021 tcatcttccg gcacgcgcag caaatactgg cgcaactcat cgcggtaggc atcggtcgcc
    10081 gtcggctggt aaagagtgtt tggatcgagc aattcatcca gcaataatgg ataacgcgcc
    10141 agctggctgg caatcatcgg cgatgcggca cacagagaaa tcaaatgttt aagcgccgca
    10201 gggaattcac tgagcaattc taaataggtg gtgcgggtaa caatccccac cagcaaggcg
    10261 gtaatgcgcg acagcgtaac ggcagcgtct tcacgcgcac agacatcact tagcagatgt
    10321 ggcatcagat ggtcgagcac ctgacgtcct cgcggcccga tagtacgctt atccagctct
    10381 ttgcggaaat cagcaatcag cgttaggacc tgtttgcgat catcctcgct aagatgcgcc
    10441 agcactggcg tagtgtcatc ttcctgcaac gcatcctgcc acagctcacg ccactgttcc
    10501 gacagcgact cttcctgagt ttcactttcg tcgtcgccaa tcaattcatt aaacacccgg
    10561 cgcacattgg tcatatgtgc ggtcagcgcc ccggtcagtt gcggccagtc agcaaagtcc
    10621 atcgcccacg ccagccgcgc acggttaagc tcatcagaag gaagcgtttg ggtttgttcg
    10681 tcgttaatgc tttgcagcag gttttccaga cgccgcagga acagatatgc cactcgcaat
    10741 tgttcagcat cgttttcaga aagcagatgc agcgcggcaa tgacgctgag cgttggcagt
    10801 aaagagcgcg actgcagcga cggttcgcgc ccaccgcgaa tgagctggaa cacctgaacg
    10861 ataaattcaa tttcgcgaat gccgcctgcg ccgagtttga tattgtcggt caaaccacgt
    10921 cgacgcactt cacgggcaat catccctttc atgttgcgca gcgactgaat cacgctgaaa
    10981 tcgatgtaac gacggaaaac aaacgggcgc agcatcgcac gcaactcgtt agcatagacg
    11041 ccttcgctat cgcccataat ccgcgccttg accatcgcgt aacgctccca gtcgcgcccc
    11101 tgctcctggt aataatcctc caacgcggca aagctcagca ccagcgggcc actttcgcca
    11161 aacggacgca gccgcatatc cacgcgatag acgaagccat cctgcgttgg ttgatccaac
    11221 actttaatca gccgctgccc catgcgggta aaaaactgcg cgttatccag ttcccggcgt
    11281 ccgccctgcg tacaaccatg ttccggccag gcaaaaatca gatcgatatc tgaggagaaa
    11341 ttcagttccc caccgcccag cttacccatg cctaaaatca gcagcggttg cgcttcgccc
    11401 tgcgcattgc acggcgttcc ccactcgcgg cagcaagcgt catacagcca gtcacgcgcg
    11461 gcgacaatca gcgtctccgc cagatggctg agctgctgca atatgctctc ttcagtaacc
    11521 aaagccagcg tttgcgccca ggcgatgcgc accataatgc gccgccggaa tagccgcagc
    11581 tcgcgcatta acccggcttc gtcactcaca ttgctgagcg cctcctgcaa ccatgccgcg
    11641 taatgctgcc attcatcagc ctgcggcgat tggctttcca gttccgtcag ccactctgga
    11701 tgcgcaatca cgctgtcctg cacaaaatca ctgaaagtaa gtactgactt cgcctgtgcg
    11761 ctaagtgatt tctcggctaa aggctctggc agccgctcaa caacggtctg ccagtactgc
    11821 tgtaacggtg aagagagcgg cttcataagc gattttatcc ttgcctgatt aacgttttcc
    11881 gctgtgcaac cagaacggtt cctgattgtt tgcctcatta cggaaatgtt caatttcaat
    11941 gcgttgcccg gtcgcaatag cgtgacgcag cccctgccag ttctccagcc aggcttgcgc
    12001 gacgacagga tcatagtaac ccgccagcaa cagtattgag tcaatatcac gcgtcagacg
    12061 tggcaactgg tcacggtagc gatcgcccaa cggctggcag aaaacgcttt tcagttcagc
    12121 ggcatggcgg gaaagatgga tatcggcaaa gcgtttgaag gaatcgctca ttttactctg
    12181 cgctttggca tctaaaaacg gctgccaggc tttgcttacc agccattcgg tcaacgccag
    12241 cttcgccatt gccgttttgg tagagtagac cgccgtcacg gcagaaaccg cagaagcgat
    12301 ggtcgcttcg cattgagtca gcaaatcacg taagtgagtg ctcgctttgc gcggcacaat
    12361 accaccgaac agcatcagtg tatgacggac cagaccaatg gctgccagca cctgttcttt
    12421 cgccgcatcg ttgccgcgta cccacagttc ttcatgatac tgccattgcg ctaacgccag
    12481 ctcgagcgcc gcttccagcc cctgttccac atcggctttt gccgcaacat gcaaaatggt
    12541 ggtcggtttg atttcacgcg ccggattgcc ctgcgccaga tgatagccac gcgccgcttt
    12601 gctcaggctg ccctggcgta atccggtttg cgataccagt tggttcgcca gtttcagcac
    12661 cgcgcgcgtg tcgccgctaa gcagttccag ttccagctca cagataggtt cagcaaattc
    12721 ccccgctttc acctccccct gatcgagagc gatttcaatt cggctgtcat cgaccgccac
    12781 cagccatttt tcgcgataaa aatcggtgct gaacagcggt tgtacgcggg aagcgagatc
    12841 ggcgggcaat tcgccgttcg gccagacttc cgtcggtaac tgcgccaggt cgagcgtcgg
    12901 ttcgctcaac gccacgttat attccgggcg ctggtgtaag ccgcctgtca ctcttcctgc
    12961 aactttcatg gtcatctcat agcgaccgtt ttcgccacga atacgtaagc ccatatcgtg
    13021 cccacgcagc cagttatccg gcgtttcgta gtaaatattc agcaactgca cggggtcatg
    13081 gtgctcgccg cccagcgtat tgagatggtc acgcaacgcc tcaacggcac tgtgattaac
    13141 aataaacttt aattcgattt cctgagccat ggcctttact tatcggttat gtcacataag
    13201 tgacgatgaa cggcgaactt aatgcgatct tttttgtcag tagatagtat tttgcgccaa
    13261 attgccatgc aacgggcaat ttgacgggcg taaaagtttg aagcagtggc aagacctgac
    13321 acagaggatt ccgattgatg tcgaatgctt tgcgtagcga cactgatgcc actactatcg
    13381 ttccactttc catgacaata acgacagcct gatgccaaaa ttacgcctga tcggattaac
    13441 tttactcgca cttagcgcca ctgccgtctc acacgctgaa gaaacgcgct atgtttctga
    13501 tgaactgaat acctgggtcc gtagcggtcc gggagatcat tatcgcctcg tgggcacggt
    13561 taacgccggc gaggaagtga ccttattaca aactgacgcc aacaccaatt atgcccaggt
    13621 gaaagacagc tctggccgta ccgcctggat cccgttgaaa caacttagca ctgagccaag
    13681 cctgcgctcc cgtgtgccag atctggaaaa tcaggtcaaa accctgaccg ataaactcac
    13741 caatatcgat aacacctgga atcagcgcac cgcagaaatg cagcaaaaag tggcgcagag
    13801 cgacagcgtg atcaacggat taaaagaaga aaatcagaaa ttgaaaaacg agctgattgt
    13861 cgcgcagaaa aaggtcgatg ccgccagcgt acagctggat gacaaacagc gcaccatcat
    13921 catgcaatgg tttatgtatg gcggtggcgt gctggggctt ggcttgctgc tcggtctggt
    13981 actgccacac ctgatcccaa cgcgcaaacg caaagatcgc tggatgaact aaatcgcctt
    14041 cttcgttgca ctgtcttatt attaggaatt gtgtatttga cgaagggatg atggcgtgaa
    14101 gatttatctg gtcggtggtg ctgttcggga tgcattgtta gggctaccgg tcaaagacag
    14161 agattgggtg gtggtcggca gtacgccaca ggagatgctc gacgcgggct accagcaggt
    14221 aggccgcgat tttcctgtgt ttctgcatcc gcaaacgcat gaagagtatg cgctggcacg
    14281 taccgaacgg aaatccggtt ccggttacac cggttttact tgctatgccg caccggatgt
    14341 cacgctggaa gatgatctta agcgtcgcga tctgaccatt aatgcgctgg cccaggacga
    14401 taacggcgag attatcgacc cgtacaacgg tctgggcgat ctgcaaaatc gtctgttgcg
    14461 ccatgtttcc cccgcttttg gcgaagatcc gttacgcgtt ctgcgcgtgg cgcgttttgc
    14521 tgcgcgttat gcccacctcg gttttcgtat tgccgatgaa actctgacgc tgatgcgcga
    14581 gatgacccat gcgggtgaac tggaacacct gacgcctgaa cgggtatgga aagagacgga
    14641 aagcgccctc accacccgca atccacaggt gttcttccag gtactgcgcg attgtggcgc
    14701 actgcgcgtt ttattcccgg aaattgacgc actgtttggc gttccggccc ctgccaggtg
    14761 gcatccggaa atcgacacgg gtattcatac cttaatgacg ctctcaatgg cggcgatgct
    14821 gagtccgcag gtcgatgtcc gtttcgcaac tttatgccac gatctcggta aaggactgac
    14881 gccgccagaa ctttggccgc gtcatcatgg tcacggtccg gcgggtgtga aattagtgga
    14941 acaactctgc cagcgtctgc gcgtgccaaa tgaaattcgc gatttagcca gactggtggc
    15001 tgagtttcac gatctcatcc acaccttccc aatgctgaac ccaaaaacta tcgtcaaatt
    15061 gtttgattcc atcgatgcct ggcgtaaacc gcagcgtgtc gagcaactgg cgctgaccag
    15121 cgaggctgac gtgcgcggca gaaccggttt tgaatcagcg gactacccgc aaggccgttg
    15181 gttgcgcgaa gcctgggaag tggcgcagtc agtgccgaca aaagcggtcg ttgaagcggg
    15241 atttaaaggt gtggagattc gcgaggagct gacccgacgg cggattgcgg cggtagccag
    15301 ctggaaggaa caacgttgcc caaagcctga ctgagtataa aactaaacgt tagcacgaac
    15361 cgtcacctcg ccccttcggg gagagggtta gggtgagggg aacagaccag caccggtgcg
    15421 aacaatcacc cccaccccgg ccctctcctt acagaccgag ggagaaaacc cggcctgctc
    15481 ccgaaccttt tctcaatcaa aagaacacaa cgtacaccgc agccgccaca ataaagcgat
    15541 aaatggcgaa cgggataaac gaaatgcgct taatcaattg caggaaggtt ttaatcgcta
    15601 tcagcgccac cacaaaagcg gtgataaacc caacggcaaa catcgggata tcgccggttg
    15661 tcaggaaccc ccagcttttg tagagatcga gcgccgttgc gcccatcatc atcggtaccg
    15721 ccagcaggaa cgagaactcg gaagcagcgt aacggctcac gcccatcagc atcccacctg
    15781 aaatggtggc cccggaacgg gagaaacccg gccacagcgc cagacactgg aaacagccaa
    15841 tcataaacgc ctgacgatag gtcatatcat caagacccgg cgcacgcggc tctttcggct
    15901 tcaggcattc ggcggcaatc agcaacaaac cgccaacgac cagtgcatac atcacattta
    15961 tcgggttaaa caatgactta atcgtgtcgt ggaacaacag ccccagtacc accgccggaa
    16021 tcatccccag caaaatgtgg atcagcgtta aacgaccttt gctttctcct tcgtgctgca
    16081 acgggcggcc aaagtggatg ccaatcaggc caaacagacg ccgccagaac atcactacta
    16141 ccgccagaat tgatcctaac tggatcacaa cttcaaaggt tttcgccgtg tcgccctcaa
    16201 accccaacaa gtgaccgaca ataatcatat ggcccgtgct ggataccggc agaaattctg
    16261 tcaatccttc gaccacaccc aatattgccg ctatcagcag cgagtgcata tcgctcatca
    16321 ataaacccct aaattataaa aatgtaacgc ttgtccgaac tactgcagta tgaccaggtt
    16381 ataaccgttt ggtttaacag ctgtaaaatt aattattttc tttcagatta ttgccacgct
    16441 caatgattac gccaacattc gccgcccgca ccactgcccc tggcttgctg agtttgatac
    16501 gcacccacgg cgagttgaag cgtgctaaca gcagctccgc cacctcttca gcaacgcgtt
    16561 ccaccagcgc aaaacgcgcc ccctcgacgt ggctgaccac cgtttctgca atgtcagcgt
    16621 aactgaggca atccgccaca tcatcacttt tcgccgcttt acggttatcc cacgctattt
    16681 cgatatcgaa cactaacttc tgttcgatgg tctgttccca gtcgtaaaca ccaatagtgg
    16741 tgattaccga aagttgctct ataaatacaa tatccatcac gtcctgcctg ctttttggct
    16801 aaccggatac cacttccggc gaaatgtgcg tattatccac agattcatcg ttgaacacga
    16861 attttcaaaa cggaacagct tatgagtgca atcgcgcctg gaatgatcct catcgcgtac
    16921 ctctgcggct ccatttccag tgccattctg gtttgccgct tgtgtgggct gcccgatccg
    16981 cgaaccagcg gctccggcaa tccaggcgca accaatgtgt tacgtatcgg tggcaaggga
    17041 gcagccgtag cagtactgat tttcgacgtt ctgaaaggaa tgttgcccgt ctggggcgcg
    17101 tatgaattag gtgtcagccc cttctggcta ggcttaattg ccatcgccgc ctgtcttgga
    17161 cacatctggc ccgttttctt cggttttaaa ggaggaaagg gcgttgctac cgcttttggt
    17221 gccatcgcac ccattggctg ggatctcacc ggagtaatgg cgggaacctg gttactgacc
    17281 gtgctattga gcggatactc gtcgctggga gcgattgtca gtgcactgat tgctccgttt
    17341 tatgtctggt ggtttaagcc acaattcacc ttcccggttt cgatgctctc ttgcctgatc
    17401 ctgctgcgtc atcatgacaa catccagcgt ctgtggcgtc gtcaggagac aaaaatctgg
    17461 acgaaattca aaagaaagcg cgaaaaggat cccgagtgat ttctggtgga tctacatgac
    17521 ctgatagcct tcatcgggct tgcccagccg ttgctggcac catgccgcca gaaattccac
    17581 gcagacacgt aatttcatgc tgcgatagag cggctcccgg taaacagccc agatattggc
    17641 gctttgtgca tactccggca atacttgcac cagtttgcca ctctccagaa acggcaacac
    17701 atcccactcg gaacgcagca taatcccttt gccctccagc gctcattgca acactatctc
    17761 gccgctattg gaggaaagat gcccgctaac cttcaccgat tttttctcct gaccgttccc
    17821 caactcccat attccgtggg tcatatcgcg ctctttggtc accagacagt catgacgact
    17881 taattcctgt aatgattgtg gctgcggata tttttgcaga tactcaggtg ctgcgcataa
    17941 tattctttta tttttcgtta acagatgcgc aatataataa tcaggaattt cgtcattaat
    18001 acgaatatcc agatcaatat tatcctgcac taaatcaatt tgccgatcga acaattcaaa
    18061 atgcacctgt aactcaggat aattgcgcat aagttcggca atggctggcg caatatggct
    18121 gcgcccaaaa ccaaaactac agccaatacg aatcatcccc tccgggcgcg ttttgatttg
    18181 cgtgacgtca tcgaccagtc gctgatattg ggtgaggatt tccagcgcat gttcgtaaca
    18241 acgttggcca ctttcagtca gcgccacgcc gcgagcggag cggttcagca gcgttgttgc
    18301 cagcgtattt tcaaggattt ggatccgctt ggtgacaaat gcaggcgtct gcccaagcgt
    18361 cgccgctgcg gcgctaaaac tcccgctgtg gacgatttct accagtacct gcaagtcttt
    18421 ggctaaaggc cagctattca gcatcgttaa ttatccgcag ttgtgataag cgcagtgtat
    18481 ttcgcaaaac attgccattc cctgctctgg cgcaatattc attcttattt tcgcgttgat
    18541 tcacgaactg ttaattacat attcacggtg gcaaaaaata taaaaccaca tttttagtgt
    18601 tagtttgtgg cggtgaattt ttccagacaa atacaaaaac tggagttgcc atgatgagcg
    18661 aaagtaataa gcaacaggca gtgaataagt tgacagagat tgtcgctaac tttaccgcca
    18721 tgatttctac ccgaatgcct gatgacgtgg tggataaact aaaacagcta aaggatgcca
    18781 aaacgtcgtc gatggggaaa attatctacc atacgatgtt cgacaacatg caaaaagcga
    18841 ttgacctgaa tcgtcctgcc tgtcaggaca ccggggagat tatgttcttc gttaaagtcg
    18901 gttcccgctt cccactgctt ggcgagctgc aaagcatact caaacaagcc gtggaagagg
    18961 caaccgtcaa agcgccacta cgtcacaatg cggtagaaat ttttgacgaa gtaaacaccg
    19021 gcaaaaatac cggtagcggc gtaccgtggg tcacctggga catcatcccc gacaatgacg
    19081 atgcggaaat cgaagtttac atggcaggcg gcggctgcac gctacctggc cgctcgaaag
    19141 tgttaatgcc gtcagaaggc tacgaaggcg tggtgaaatt cgtcttcgaa aatatctcca
    19201 ccctcgccgt aaacgcctgt ccaccggtac tggtgggcgt gggcatcgcc acctcggtgg
    19261 aaaccgccgc cgtactctcg cgtaaagcca ttttgcgtcc gattggcagc cgccatccca
    19321 atccaaaagc ggcagagctg gagctacgcc tggaagaagg actcaaccgt ctggggattg
    19381 gtccacaagg gctgaccggc aacagttcag tgatgggcgt gcatatcgaa tctgccgccc
    19441 gccatccgtc aaccatcggc gttgctgtct ctaccggctg ctgggcgcat cgtcgcggca
    19501 cgctgctggt tcatgccgat ctcacctttg aaaatctgtc tcacaccagg agcgcgttat
    19561 gaaaaagatc ctgacaaccc cgatcaaagc tgaagatctg caagatattc gcgtcggcga
    19621 tgtgatctac ctgaccggta cgctggtgac ctgccgcgac gtttgtcacc gccgtttgat
    19681 cgaactgaaa cgtccgatcc cttacgatct caacggcaaa gcgattttcc acgctggccc
    19741 catcgtgcgc aaaaacggcg acaaatggga gatggtctcc gtcggcccga caaccagtat
    19801 gcgtatggaa agttttgaac gtgaatttat tgagcagacc ggcgtgaaac tggtggtcgg
    19861 caaaggcaga atggggccgc tgaccgaaga aggctgtcag aaattcaagg cgctacatgt
    19921 gattttccct gcaggctgcg cggtgctggc ggcaacccag gtggaagaga ttgaagaagt
    19981 gcactggaca gagctcggaa tgccggagtc gctgtgggtt tgccgggtca aagagttcgg
    20041 cccgctgatt gtctctattg atacccacgg caacaacctg atagccgaaa acaaaaagct
    20101 gttcgccgaa cgccgcgatc ccatcgtgga agagatctgc gagcacgtcc attacatcaa
    20161 ataaccctcc cggagaggct cgccccctct cctttttcgc aggcataaca cgatgaaacc
    20221 ttccactgaa tggtggcgat accttgcgcc gctggcggtc atcgccatta ttgctctaat
    20281 tccggttccc gcagggctgg agagtcatac ctggctctac tttgccgttt ttactggcgt
    20341 gatcgttgga ctgatcctcg aacccgtgcc gggtgccgtg gtggcgatgg tgggtatctc
    20401 catcatcgcc atactctctc cctggctgct gttcagcccc gaacagctcg cccagccagg
    20461 ctttaaattc actgcaaaat ccctctcgtg ggcggtttcc ggtttttcta attcggttat
    20521 ctggctgatt ttcgccgcct ttatgtttgg cacaggctat gaaaaaaccg ggcttggacg
    20581 ccgcattgcg ctgtttctgg tgaaaaagat gggacatcgc acgctgtttc tcggctatgc
    20641 ggtgatgttc tccgagctga tcctcgcacc tgtaacaccg tccaactcgg cgcgtggtgc
    20701 ggggattatc tatcccatca tccgtaacct gccaccgctc tatcaatcac aaccaaacga
    20761 cagcagttcg cgcagcattg gctcgtacat catgtggatg gggattgttg ccgactgcgt
    20821 gaccagcgcc attttcttga cggcgatggc accaaacttg ctgttaattg gactgatgaa
    20881 aagcgcatct cacgccacgc tgagttgggg cgactggttc ctcgggatgt tgccgctcag
    20941 tattttgctg gtgctgctgg ttccctggct ggcttatgtg ctgtacccgc cggtactgaa
    21001 gtctggcgat caggtgccgc gctgggcaga gacggaactg caggcaatgg gcccgctctg
    21061 ttcgcgtgaa aaacggatgc tggggctgat ggtaggcgcg ctggtgctgt ggattttcgg
    21121 cggtgattat atcgatgccg cgatggtcgg ttacagcgtg gtggcactga tgctgcttct
    21181 gcgcattatc agttgggacg acattgtcag taataaagcg gcgtggaacg ttttcttctg
    21241 gctggcctcg cttatcaccc tcgctaccgg actcaacaac actggtttta ttagctggtt
    21301 tggcaaactg ttagcaggca gcttaagcgg ttattcgcca acgatggtga tggtggcgtt
    21361 gattgtggtg ttttatctgc tgcgctactt tttcgccagc gccacggcgt atacctccgc
    21421 cctcgcaccg atgatgattg ccgctgcgct ggcgatgccg gaaatcccgc tgccggtgtt
    21481 ctgcctgatg gttggcgcgg caattggtct ggggagcatt cttacaccat acgccaccgg
    21541 acccagtccg atttactacg gtagtggtta tctgccaacg gtggattact ggcgactggg
    21601 ggcgattttt ggactgatat tcctcgtgtt gctggtgatt accggcttgc tgtggatgcc
    21661 cgtggtgttg ctttaagtga ggacggtgcc ggaaattgct ctccggcacc ggatagtttt
    21721 acgcagccgg taactctgcc agtggccagc gcggacgcac gctaacgccg agatccgccg
    21781 tcgcgcctgc tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac
    21841 aaaattccgg acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tccgccagct
    21901 tcgcccgtaa cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg
    21961 tctgatccaa cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg
    22021 cgcgggcgat atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc gtgttcgccg
    22081 caaaggtttt cagaccagaa aagctgaaat ccagccccgg acggtcggtc atcggacgcg
    22141 ggaaaacaaa gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggtccgccag
    22201 gataatccag ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga
    22261 tagactcgcc gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac
    22321 cgccggacac cagcagcgcg acaaacggaa attccggcgg gttatcttcc agcatcggcg
    22381 ctaacagatg cccttccata tggtgtaccg ggattgccgg aacgttccag gcaaacgcca
    22441 gagaacgccc cacggttgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat
    22501 aggccacagc atcaatgtct tttgccgtta agccagactc ctttagcgcc gcctggatca
    22561 acggtacggt tttgcgcacg tgatcgcggg aggccagttc aggcacgacg ccgccgtagt
    22621 cagcgtgcaa tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt
    22681 aaatggcgat gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt
    22741 ttacctcgct ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc
    22801 gcccctggag aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaggc
    22861 cgcggtgctt tacaaagcag cagcaattgc agtaaaattc cgcaccattt tgaaataagc
    22921 tggcgttgat gccagcggca aaccgaatta atcaaaggtg agaggcacat gccggtaatt
    22981 aaagtacgtg aaaacgagcc gttcgacgta gctctgcgtc gcttcaagcg ttcctgcgaa
    23041 aaagcaggtg ttctggcgga agttcgtcgt cgtgagttct atgaaaaacc gactaccgaa
    23101 cgtaagcgcg ctaaagcttc tgcagtgaaa cgtcacgcga agaaactggc tcgcgaaaac
    23161 gcacgccgca ctcgtctgta ctaattcccc gagagcgttg ctctccgatc agaccgagtt
    23221 gtagttgtaa ggccgtgctt ccgaaaggaa tgcgcggctt attttcgttt atgaattgct
    23281 aaaaatcggg gcctatggct ggacgaatcc cacgcgtatt cattaatgat ctgctggcac
    23341 gcactgacat cgtcgatctg atcgatgccc gtgtgaagct gaaaaagcag ggcaagaatt
    23401 tccacgcgtg ttgtccattc cacaacgaga aaaccccgtc attcaccgtt aacggtgaga
    23461 aacagtttta ccactgcttt ggatgtggcg cgcacggcaa cgcgatcgac ttcctgatga
    23521 actacgacaa gctcgagttc gttgaaacgg tcgaagagct ggcagcaatg cacaatcttg
    23581 aagtgccatt tgaagcaggc agcggcccca gccagatcga gcgccatcag aggcaaacgc
    23641 tttatcagtt gatggacggt ctgaatacgt tttaccaaca atctttacag caacctgttg
    23701 ccacgtctgc gcgccagtat ctggaaaaac gcggattaag ccacgaggtt atcgctcgct
    23761 ttgcgattgg ttttgcgccc cccggctggg acaacgtcct gaagcggttt ggcggcaatc
    23821 cagaaaatcg ccagtcattg attgatgcgg ggatgttggt cactaacgat cagggacgca
    23881 gttacgatcg tttccgcgag cgggtgatgt tccccattcg cgataaacgc ggtcgggtga
    23941 ttggttttgg cgggcgcgtg ctgggcaacg atacccccaa atacctgaac tcgccggaaa
    24001 cagacatttt ccataaaggc cgccagcttt acggtcttta tgaagcgcag caggataacg
    24061 ctgaacccaa tcgtctgctt gtggtcgaag gctatatgga cgtggtggcg ctggcgcaat
    24121 acggcattaa ttacgccgtt gcgtcgttag gtacgtcaac caccgccgat cacatacaac
    24181 tgttgttccg cgcgaccaac aatgtcattt gctgttatga cggcgaccgt gcaggccgcg
    24241 atgccgcatg gcgagcgctg gaaacggcgc tgccttacat gacagacggc cgtcagctac
    24301 gctttatgtt tttgccggat ggcgaagacc ctgacacgct ggtacgaaaa gaaggtaaag
    24361 aagcgtttga agcgcggatg gagcaggcga tgccgctttc cgcatttctg tttaacagcc
    24421 tgatgccgca agttgatctg agtacccctg acgggcgcgc acgtttgagt acgctggcac
    24481 tgccattgat atcgcaagtg ccgggcgaaa cgctgcgaat atatcttcgt caggaattag
    24541 gcaacaaatt aggcatactt gatgacagcc agcttgaacg attaatgcca aaagcggcag
    24601 agagcggcgt ttctcgccct gttccgcagc taaaacgcac gaccatgcgt atacttatag
    24661 ggttgctggt gcaaaatcca gaattagcga cgttggtccc gccgcttgag aatctggatg
    24721 aaaataagct ccctggactt ggcttattca gagaactggt caacacttgt ctctcccagc
    24781 caggtctgac caccgggcaa cttttagagc actatcgtgg tacaaataat gctgccaccc
    24841 ttgaaaaact gtcgatgtgg gacgatatag cagataagaa tattgctgag caaaccttca
    24901 ccgactcact caaccatatg tttgattcgc tgcttgaact gcgccaggaa gagttaatcg
    24961 ctcgtgagcg cacgcatggt ttaagcaacg aagaacgcct ggagctctgg acattaaacc
    25021 aggagctggc gaaaaagtga tttaacggct taagtgccga agagcgatcg ggaagccccc
    25081 gacagccgca ctgagaggca gcggcaaata tataagtacg ccctcgtaat tatcgttggc
    25141 ggtaaacaac cgttggattt cagcgttaac ggctgaagga catcgggtca atcgcccaac
    25201 accaacctca tgaaataagt gtggataccg tcttatggag caaaacccgc agtcacagct
    25261 gaaacttctt gtcacccgtg gtaaggagca aggctatctg acctatgccg aggtcaatga
    25321 ccatctgccg gaagatatcg tcgattcaga tcagatcgaa gacatcatcc aaatgatcaa
    25381 cgacatgggc attcaggtga tggaagaagc accggatgcc gatgatctga tgctggctga
    25441 aaacaccgcg gacgaagatg ctgccgaagc cgccgcgcag gtgctttcca gcgtggaatc
    25501 tgaaatcggg cgcacgactg acccggtacg catgtacatg cgtgaaatgg gcaccgttga
    25561 actgttgacc cgcgaaggcg aaattgacat cgctaagcgt attgaagacg ggatcaacca
    25621 ggttcaatgc tccgttgctg aatatccgga agcgatcacc tatctgctgg aacagtacga
    25681 tcgtgttgaa gcagaagaag cgcgtctgtc cgatctgatc accggctttg ttgacccgaa
    25741 cgcagaagaa gatctggcac ctaccgccac tcacgtcggt tctgagcttt cccaggaaga
    25801 tctggacgat gacgaagatg aagacgaaga agatggcgat gacgacagcg ccgatgatga
    25861 caacagcatc gacccggaac tggctcgcga aaaatttgcg gaactacgcg ctcagtacgt
    25921 tgtaacgcgt gacaccatca aagcgaaagg tcgcagtcac gctgccgctc aggaagagat
    25981 cctgaaactg tctgaagtat tcaaacagtt ccgcctggtg ccgaagcagt ttgactacct
    26041 ggtcaacagc atgcgcgtca tgatggaccg cgttcgtacg caagaacgtc tgatcatgaa
    26101 gctctgcgtt gagcagtgca aaatgccgaa gaaaaacttc attaccctgt ttaccggcaa
    26161 cgaaaccagc gatacctggt tcaacgcggc aattgcgatg aacaagccgt ggtcggaaaa
    26221 actgcacgat gtctctgaag aagtgcatcg cgccctgcag aaactgcagc agattgaaga
    26281 agaaaccggc ctgaccatcg agcaggttaa agatatcaac cgtcgtatgt ccatcggtga
    26341 agcgaaagcc cgccgtgcga agaaagagat ggttgaagcg aacttacgtc tggttatttc
    26401 tatcgccaag aaatacacca accgtggctt gcagttcctt gacctgattc aggaaggcaa
    26461 catcggtctg atgaaagcgg ttgataaatt cgaataccgc cgtggttaca agttctccac
    26521 ctacgcaacc tggtggatcc gtcaggcgat cacccgttct atcgcggatc aggcgcgcac
    26581 catccgtatt ccggtgcata tgattgagac tatcaacaaa ctcaaccgta tttctcgcca
    26641 gatgctgcaa gagatgggcc gtgaaccgac gccggaagaa ctggctgaac gtatgctgat
    26701 gccggaagac aagatccgca aagtgctgaa gatcgccaaa gagccaatct ccatggaaac
    26761 gccgatcggt gatgatgaag attcgcatct gggggatttc atcgaggata ccaccctcga
    26821 gctgccgctg gattctgcga ccaccgaaag cctgcgtgcg gcaacgcacg acgtgctggc
    26881 tggcctgacc gcgcgtgaag cgaaagttct gcgtatgcgt ttcggtatcg atatgaacac
    26941 cgaccacacg ctggaagaag tgggtaaaca gttcgacgtt acccgcgaac gtatccgtca
    27001 gatcgaagcg aaggcgctgc gcaaactgcg tcacccgagc cgttctgaag tgttgcgtag
    27061 cttcctggac gattaatcgg taggccggat caggcgttac gccgcacccg gcactagtcc
    27121 ctctgcacaa acgccacctt ttcggtggcg tttttatcgc ccacgcacta ccagcgcctg
    27181 gtccagctcg cgatacgctt caaccagttt ctctagtgaa acgcgactta aaccgctggg
    27241 atttggcagc acccaaatct gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc
    27301 accgcgctgg ctgaatccct gttcatatgc tcgtttgccc agaatcgcca acgcctgcgg
    27361 ctgataatct tcaatttttt caatcagctt acgcccacct gcgtgcagct cctgctttga
    27421 aacttcattg gcttgcaccg ttggacggtc taccagtttg gtgacgccac aacgataatc
    27481 cagcagatgc tgtgcctcct gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat
    27541 caccttccag aagcgatttg ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag
    27601 cccagggttg ataccgcaaa acacgacccg taaccctgga gccaaaatat cctcaaccat
    27661 atttactcct ttctaacatc accaggaaaa gtataacgga ttgaaaatac attgtttata
    27721 aaaacagcag gcgcacggta atggctggat tgcgacacgg agttacttta taatccgcta
    27781 ccatggcccc ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgttggttc
    27841 aaacccaaca ggggccacca tatacaacaa agggttagct aataaatcag ctaacccttt
    27901 ttcttttacc tttgaattgg gtcaggtaag ctttttctcc atctgactca gatggctgat
    27961 agttgccccg gataatgctt cgcaatgatg tcatttagcc gatcaaccag ttccggtttt
    28021 ctgaacgtga tgtgtgcgga gcccttctta aagtatctga tgctgaacat ctcatcttca
    28081 tagtcctctt taccctgaac agactgaatg tgatcatcca gacgaagggt gatgttttcc
    28141 cggttgtcgg ggagcggttt gccgctgaac agatgcagca ttctttccag gtcggcgagt
    28201 ccatctgcct gttgcccggt gataaggtga aatccccatc tgtcccacct caccaggttg
    28261 ttgacgataa ttttactgcc aaatttacag ggggagttgg ttttgtaatt ccagctcagc
    28321 cctctgaaga cgttgatcac tcctcgctca aacacttcat ctttattctg gtgcagttgc
    28381 tcaaatgtgc tccaaatgtt cgcttcactg atttccggaa aattatcgta ctccagtgat
    28441 cggtaccatg tatcacgggc ctgcgcatcc attaatgaca gcatcccgga acgttgcatc
    28501 aggtcacgcc agatttcgcg gtcaagattg cgggtgatgg ctttcattgc ggtttccggt
    28561 ttctccatga gccagcagcc acagcgaaaa tcctgtttca tggcccagtc cagagcagtt
    28621 ttacctccaa tactttctgt cagtaccgag atttctacca gttgtcggat aagtgtgtcg
    28681 atttgcagca gtgaactctt tcgtacagcc agaatgtggc tgactgaggt ggagcagata
    28741 acgtccgtat gcccgttgac cacttccgga cgggagactg tgattgcaga cataatcgat
    28801 attccttttc gcagataaat gaaaacgcca gccctgttca ggctggcgtc agtggaatga
    28861 agtatggtga gtgagactca ccgcttttgt tgcagaaact gcagttggcg caggatacgg
    28921 atatcttccc tggcctgttc aatacagggc aacagtgctg ccgaatccgt tttatcctca
    28981 tcgttgtcga agataatccc tgattcgcag tcaatattgt cctgcagcca cgcaatcaga
    29041 atatccagcg ccgtttctgt ggttaataat ttcatccagt gtcttttgtc ctgattacca
    29101 gtcgaaagtg ggtaagcccg gcaggcattc agcgctggca tccagatgaa tgagactgaa
    29161 gccataacgc cgggtgagag tgacgaccag acgacggaac gtttttgaca ggcccaggcg
    29221 cttaagccgc agaaccgggt atgaccacgc accggtacgt aacagatatc cgctacctgt
    29281 gaaatgaatc cattctgatt cgccggtgtc attcgtctga tgtgacagcg aatacaacca
    29341 gacgttgtcc tttgccgtga tatgtgcggt actgcagcgt atgccggtca gggcagcaaa
    29401 tggtggcgtg cgctctgctg atgtccggtc agattcatca acaatacgaa gggagtagcc
    29461 attgtcgcag gccacgttaa tcagttcagc gagattaaca ccatcgacgt caacgacaat
    29521 gcgtcccatg ttcagcgcct gcacgttaac gctgtctgct tccagcgtca gggaaaggtt
    29581 cattgtttcg cctcctccgg atgcctgccc agggtaatgt tatttaccgt tctgtaattg
    29641 tcgcgggtca tcaggccggt tgccctgcgg gcccggagga tatcgatgct gttgattaac
    29701 tgcgacggga ctcctgcgct gaatcccggc tggtcagttc gtaccagcgc gtatttttca
    29761 acgagaaagt tcaccgcgtc acacagtgaa atccctgcct caatatgctg ctcaatcaca
    29821 cgttcatcag agaacggtgt gtcattcagc gtgaggccat agtgctggtc cagcagtcgg
    29881 gtgagcagtg tctgccagat ttcaaccgga gacgggcagc gcgatgccgc ccgtacgtga
    29941 gtgtcgggta atgttttcat actgaagatt ttcctgatat gcaaagataa aaatgggaaa
    30001 gtggcgtggc gaaagcacca ggccggagca gagggttatt ccggtggttt actttttcat
    30061 atcgggcgtc ggataaacag ccagataaac gtaaccacag gagccgaggg tgtcggcatc
    30121 gcaggtcagc cctttcgcat acagtgccac ggtatgctga tggcggggat tcagttcacc
    30181 gctggtgagc atgagttcca gttgtttcat cagcagcgga aaggcctggt ccaggtggta
    30241 cgcatccgcg tcgctgaacc ggcctctgat accggcgcgg tcgtcaaggt aatgcaaccg
    30301 gttaccctcc tgcaccagac gtgccccgaa acagggcgtc acggtgcagg gcagtcccca
    30361 ccaggggcgg ttgtgattgt cgttgggata atttgtctca tggagtgtgt ctgacacgat
    30421 aaaatcctca caa
//
LOCUS       DAZKIV010000040        30244 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZKIV010000040 DAZKIV010000000
VERSION     DAZKIV010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30244
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.040"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..258)
                     /gene="hybG"
                     /locus_tag="ACHOT6_001818"
     CDS             complement(10..258)
                     /gene="hybG"
                     /locus_tag="ACHOT6_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFP9947048.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(271..612)
                     /gene="hypA"
                     /locus_tag="ACHOT6_001819"
     CDS             complement(271..612)
                     /gene="hypA"
                     /locus_tag="ACHOT6_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFP9947049.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(605..1093)
                     /gene="hybE"
                     /locus_tag="ACHOT6_001820"
     CDS             complement(605..1093)
                     /gene="hybE"
                     /locus_tag="ACHOT6_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFP9947050.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPEQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1086..1580)
                     /locus_tag="ACHOT6_001821"
     CDS             complement(1086..1580)
                     /locus_tag="ACHOT6_001821"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFP9947051.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGELPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1580..3283)
                     /gene="hybC"
                     /locus_tag="ACHOT6_001822"
     CDS             complement(1580..3283)
                     /gene="hybC"
                     /locus_tag="ACHOT6_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFP9947052.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3280..4458)
                     /gene="hybB"
                     /locus_tag="ACHOT6_001823"
     CDS             complement(3280..4458)
                     /gene="hybB"
                     /locus_tag="ACHOT6_001823"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFP9947053.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4448..5434)
                     /gene="hybA"
                     /locus_tag="ACHOT6_001824"
     CDS             complement(4448..5434)
                     /gene="hybA"
                     /locus_tag="ACHOT6_001824"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFP9947054.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5437..6555)
                     /gene="hybO"
                     /locus_tag="ACHOT6_001825"
     CDS             complement(5437..6555)
                     /gene="hybO"
                     /locus_tag="ACHOT6_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFP9947055.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6744..7031)
                     /gene="yghW"
                     /locus_tag="ACHOT6_001826"
     CDS             complement(6744..7031)
                     /gene="yghW"
                     /locus_tag="ACHOT6_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFP9947056.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7150..8037)
                     /gene="yghX"
                     /locus_tag="ACHOT6_001827"
     CDS             complement(7150..8037)
                     /gene="yghX"
                     /locus_tag="ACHOT6_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFP9947057.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8194..9234
                     /gene="gpr"
                     /locus_tag="ACHOT6_001828"
     CDS             8194..9234
                     /gene="gpr"
                     /locus_tag="ACHOT6_001828"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFP9947058.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9274..9768)
                     /locus_tag="ACHOT6_001829"
     CDS             complement(9274..9768)
                     /locus_tag="ACHOT6_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFP9947059.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9959..10843
                     /gene="yghA"
                     /locus_tag="ACHOT6_001830"
     CDS             9959..10843
                     /gene="yghA"
                     /locus_tag="ACHOT6_001830"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9947060.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGCKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11115..11540)
                     /gene="exbD"
                     /locus_tag="ACHOT6_001831"
     CDS             complement(11115..11540)
                     /gene="exbD"
                     /locus_tag="ACHOT6_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFP9947061.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11547..12281)
                     /gene="exbB"
                     /locus_tag="ACHOT6_001832"
     CDS             complement(11547..12281)
                     /gene="exbB"
                     /locus_tag="ACHOT6_001832"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9947062.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERQVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12533..13720
                     /gene="metC"
                     /locus_tag="ACHOT6_001833"
     CDS             12533..13720
                     /gene="metC"
                     /locus_tag="ACHOT6_001833"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFP9947063.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13860..14519
                     /gene="yghB"
                     /locus_tag="ACHOT6_001834"
     CDS             13860..14519
                     /gene="yghB"
                     /locus_tag="ACHOT6_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFP9947064.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14559..15458)
                     /gene="yqhC"
                     /locus_tag="ACHOT6_001835"
     CDS             complement(14559..15458)
                     /gene="yqhC"
                     /locus_tag="ACHOT6_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFP9947065.1"
                     /translation="MKREEICRLLADKVNKLKIKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15652..16815
                     /gene="yqhD"
                     /locus_tag="ACHOT6_001836"
     CDS             15652..16815
                     /gene="yqhD"
                     /locus_tag="ACHOT6_001836"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFP9947066.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16920..17747
                     /gene="dkgA"
                     /locus_tag="ACHOT6_001837"
     CDS             16920..17747
                     /gene="dkgA"
                     /locus_tag="ACHOT6_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFP9947067.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17947..18873
                     /locus_tag="ACHOT6_001838"
     CDS             17947..18873
                     /locus_tag="ACHOT6_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFP9947068.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18924..19181
                     /gene="yqhH"
                     /locus_tag="ACHOT6_001839"
     CDS             18924..19181
                     /gene="yqhH"
                     /locus_tag="ACHOT6_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFP9947069.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19224..21443)
                     /locus_tag="ACHOT6_001840"
     CDS             complement(19224..21443)
                     /locus_tag="ACHOT6_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFP9947070.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21554..22966)
                     /gene="ftsP"
                     /locus_tag="ACHOT6_001841"
     CDS             complement(21554..22966)
                     /gene="ftsP"
                     /locus_tag="ACHOT6_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFP9947071.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRVHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23041..23778)
                     /gene="plsC"
                     /locus_tag="ACHOT6_001842"
                     /gene_synonym="parF"
     CDS             complement(23041..23778)
                     /gene="plsC"
                     /locus_tag="ACHOT6_001842"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFP9947072.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24011..26269)
                     /gene="parC"
                     /locus_tag="ACHOT6_001843"
     CDS             complement(24011..26269)
                     /gene="parC"
                     /locus_tag="ACHOT6_001843"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFP9947073.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26815..27297)
                     /locus_tag="ACHOT6_001844"
     CDS             complement(26815..27297)
                     /locus_tag="ACHOT6_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFP9947074.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27350..27742)
                     /gene="ygiW"
                     /locus_tag="ACHOT6_001845"
     CDS             complement(27350..27742)
                     /gene="ygiW"
                     /locus_tag="ACHOT6_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFP9947075.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27894..28553
                     /gene="qseB"
                     /locus_tag="ACHOT6_001846"
     CDS             27894..28553
                     /gene="qseB"
                     /locus_tag="ACHOT6_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFP9947076.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28550..29899
                     /gene="qseC"
                     /locus_tag="ACHOT6_001847"
     CDS             28550..29899
                     /gene="qseC"
                     /locus_tag="ACHOT6_001847"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFP9947077.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29945..>30244)
                     /locus_tag="ACHOT6_001848"
     CDS             complement(29945..>30244)
                     /locus_tag="ACHOT6_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFP9947078.1"
                     /translation="ALLYIMLTYWLIDAFFAFNKYDWMLESGDNICSIPSVSGEDRIL
                     QAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         7464 a         8077 c         7645 g         7058 t
ORIGIN      
        1 agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt ctaatgtggc
       61 tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca gcacccactg
      121 gcccagtaga tcggcagggt taccttcaca aatcagggcg atattcacgt cgcgcttgat
      181 accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca gcacctggcc
      241 tggaacgcca atacacatta ttaactccgg ttattcaact tcaatacttt tgacgatcag
      301 cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact gcgcatcgtg
      361 ctgatgaatc tccaccacct ggctgcaatc ccagcaccaa gcctgggcgg gtttatagac
      421 gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa aactaaaacg
      481 gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg cggtgacgcg
      541 cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct ggcaaagaga
      601 caactcatgc attttcgcca ctcctgcgac caaacagcag ggcgcgacgc cctgcgtgcg
      661 gtacatccgg attcgtgact ggcagcgaaa ggatcattcg tgcgcagtca tccgtcaggc
      721 gttggccctc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa caggagagat
      781 attgcgaaac accgtccagt tcgccaacgg taaaggtcat ggtgccatac ggcagttgca
      841 gaccgatttt ttcactcact ttgcgcagcg gccagagttg ctccgggccg gggaagataa
      901 ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg aacagcgtaa
      961 agtcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc atcgaacgcc
     1021 gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg aaaccagcta
     1081 tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc gcgcagcgca
     1141 gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt taagccgatg
     1201 tgtggctcca gcgattccgg gatcacgccg accaaggtca gctttttcgg taactcgccg
     1261 gtgaagcgca gggccgacaa gacgtcggcc aggccaagct gatgcggaga gattttgttg
     1321 gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg cgtgttcttt
     1381 ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc gccaagcagc
     1441 tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat gtatcgttgc
     1501 tctaacgctt cgacaatccg cacaccgatg gcttcatcgg tcagcaaaat attgccgacc
     1561 cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg ccgtcggcat
     1621 ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta cgcaccactt
     1681 ccagcggttt attcggatca gcaaccggtg tacccaccag cgactgctcg taaggaccga
     1741 cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaact gcctggtagt
     1801 tgctgatgat accgtctttg atcaccatcc agtgagagag cataccgcgc ggcgcttcga
     1861 ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggtttcaca aaggtggtgt
     1921 gatcgccttt gccgatattg gtgatcagtg cactgtattg gttttgcagg atatcctgca
     1981 attcacagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc agctgtgcca
     2041 cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc agtttgttct
     2101 gggtagattc tcgacctgcc gccagtttca ccagcatatt agccagcgga cccacttcta
     2161 ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg tcagaccaac
     2221 catcataagc cggaatggtg gtaccttccc acggcgcctg tggcgcttcg tctttatacc
     2281 aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa tgagaagtga
     2341 tcggacgata cgaggacaga tccgcattct caatgtagcc gcccgggaac aggaagctgc
     2401 cgtttttgct gtcggtcggg aattccggca cgctcaggta gttcaccgca cctttaccgc
     2461 gcgtcagcca ttccgggtag aacgcggcga taaccgcggt atcaacctta taaacctgct
     2521 caacaaagtc gctcagtttg tcgatgaaag acttgatgta catcaggcgc tcaaggttca
     2581 acacgcccaa cccgtcgagg ttgattgggt tcgcgacacc acccactgcc aggttctgaa
     2641 tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc tggcactcca
     2701 gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt ttcatcgctg
     2761 ggtgacccca gtagccatta gcgaaaatac ccaactgacc gctggcaacc agatctttga
     2821 tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc gaaacgcctt
     2881 tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg tccacccagt
     2941 ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc gcagccagaa
     3001 tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca ctttctgccg
     3061 cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt tgcacaatca
     3121 tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc cacatggtac
     3181 cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg cgtaaatgcc
     3241 cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc tcgcctcatg
     3301 acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa tgagtacgat
     3361 gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt cccaggtcgg
     3421 gaagtaggcg taaccgccgc ccgggttgaa tgccaccagc gaataggtca gacgccaggt
     3481 tgcacaacct aacagtgcgc tcagtgctga caggaacagc atgcgggaat cattacgcag
     3541 cttcgccaca cgcagaacca ccagcgggaa gagcatcagc aggacttcaa tccagaacat
     3601 cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga taagctcgcc
     3661 aaagcgcagc acgatgaaaa tcgccagcaa cacactgatg gtgttggtca gcttgacgaa
     3721 cagactcttt tcatccggac cgttgccacg cagacccgcc tgcaccagcg aaccttcaaa
     3781 gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg gcaacatttc
     3841 atagctctgc cacaacggat gcaccttgta gcccgccgag atcatcagtg accccattga
     3901 agactggtgc atggtcggca acagcgcgcc aagtgcaatg atgaagaaca tcaccttgtt
     3961 cagacgcttg agagaaacct tccagcccag acgttcaaac agtgccggag caaactccag
     4021 tgccatcacg ccgatataga tggtcataca gaccgccgtc tcgaacagta ccgagttcac
     4081 gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca cgtcgatagt
     4141 gatcgacaag ccccccagtg agtaaccaaa cagactcgcc aacagcgccg gacgcaccag
     4201 cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc agccgccaca
     4261 ggcaaagccg gtaccaatca gcaggtcaaa cgcgatccac acgccccacg ggaagccgcc
     4321 gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca ggagcataca
     4381 gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc cgcccagcgg
     4441 ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg gtgttgcgac
     4501 gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct ttatacaggg
     4561 tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc ggcaggtcga
     4621 gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc tctttctcgc
     4681 cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca gatttcagcg
     4741 tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt ttcgcctccg
     4801 ccatcagctc ttcacgcgta ccaaaaatta ccgcgcccgc cgggcacact tctacgcagc
     4861 caggcaaacc gcctttatcg agacgttcca cacctttctg gttgcacaac tcgcacttat
     4921 gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtatggg caggcgacca
     4981 tgcagtaacg gcaaccggtg cacacatctt tgtcgtaatg gacaatgccg gttttcggat
     5041 cttttttcag cgcagagacc gggcacacag agacacagtt cggatcgacg cagtgcatac
     5101 actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc cctgtgccgc
     5161 tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg ttcgaccagg
     5221 tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg gtgacgcaag
     5281 cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc ggaattggcg
     5341 ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc aatgccccgc
     5401 aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg agtcagcgtt
     5461 atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac cggcaaccag
     5521 cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc cctctttagc
     5581 gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa gctgatggat
     5641 gcctttatgg aagccgatac cttcttcgtt acagccatag catgggtgac caatcgctac
     5701 cggccacacg ccgccaacat cgcagaattg cagcgttgag cagttgccgt aagtttctgg
     5761 ccctttacag ccgaggtggt acaggcacca gccttcacgg tggccttcat caccgaactc
     5821 tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt gaatcagacg
     5881 gccataggcg aaggtcggac gatttttgtc atccagtttc ggcggtttgc cgtaagtgat
     5941 gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg gaatattgat
     6001 aacggttttg cctggcagaa cttcttgcag gctgactgcg ccagttgggt taactccagc
     6061 tgcggcaaca ccgccccacg cagagcagga accgatagcg ataatcgctg ctgcgccttc
     6121 tgccgctttg cggatgtgat ccacaatcgg ctcaccagca accatgcaat aaataccgtt
     6181 atcttttaat gggatggaac catccacaac caacacatac tgccctttgt acttctcaag
     6241 tgcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca cttcgtgata
     6301 ctccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg cacgaagcag
     6361 agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg gacgctgcgg
     6421 gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca tggtggcggc
     6481 taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat ggatgagggt
     6541 gttatctcca gtcatttata gttattccgt tgcgaagacc tggcatatat tttgcctcaa
     6601 tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat ggttatcgat
     6661 atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt aattaattaa
     6721 agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat aaaaaagcgc
     6781 aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt atccagtcca
     6841 tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc ggtcttttcg
     6901 tacatccggt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca gaattttgtc
     6961 acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc tttaccaaaa
     7021 tggttattca tcgttcacct cattcgctat atatcgccat atatagcgat attttaggca
     7081 aagaacgact tttgcgcacg ctattttttc atgcttgttt aagacgtaat ccagtaagag
     7141 ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc aggcaagatc
     7201 ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat taacccccgg
     7261 atagatatac gcctcataaa ccttattatt ggctttcaac gccgcctcgt aagcaggcca
     7321 gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg cttcaatctt
     7381 cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg ccagttccgg
     7441 atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg taatacccac
     7501 tttgcctgcc gcttgcggat agcgttgcat aaactcaatt gcggcaaaga aatcattcat
     7561 cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat ttcccggata
     7621 acctccaacg gaacttaagc cgtcaggtgc cagggcgata taccccgcct tcgccactcg
     7681 ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca ccaccacggc
     7741 tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct cgccgtgacc
     7801 atttggcgaa ggatacgtga tgtactcagc aacaatctcc gggtcggtaa actctacctg
     7861 agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg tcatcccgcc
     7921 caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gagattttcc cgtgagcgta
     7981 atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac gcggcatcgt
     8041 tgcactcctc aactggtgtt tttttaagca aagcataagc acgtgttttt gcccagtttt
     8101 tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa ctagaaacat
     8161 tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa tcccgaacgt
     8221 tacgggcaga tgcaataccg ctattgcgga aaaagcggtt tacgcctgcc cgcgttatcg
     8281 ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg tgcgatcctg
     8341 cgtaaagcgt ttgatttggg cattacgcac tttgatttag ccaacaatta cgggccgcct
     8401 ccaggaagcg cagaagagaa ctttggtcgc ctgctgcggg aggattttgc cgcttatcgc
     8461 gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc ttacggctct
     8521 ggcggttcac gtaaatacct gctcgccagc ctcgatcaaa gcctgaagcg tatggggctt
     8581 gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat ggaagaaacc
     8641 gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg gatctcctct
     8701 tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa aattccgctt
     8761 ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag cggcctgctg
     8821 gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc tcagggattg
     8881 ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgtatcg tgaagggaat
     8941 aaagttcgtg gtctgacacc gaaaatgctt accgaagcca acctcaacag cctgcgctta
     9001 ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt aagctggttg
     9061 ttgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga gcaactagag
     9121 gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc gcagattgat
     9181 cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa atgacctgtt
     9241 aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag tcagtcggtc
     9301 aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga cgtaccacat
     9361 cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaggt gaatggaaga
     9421 aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca ttttccccag
     9481 ccagttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat tctcataacc
     9541 ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca gcgacagcaa
     9601 cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt gaataatctc
     9661 ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt acacgggggc
     9721 aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac attcctgtcg
     9781 tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa cagcaaatca
     9841 acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc tggttccctt
     9901 atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga gaaaaacgat
     9961 gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca aacagaaaca
    10021 accgacgcca ggcatccagg cgaagatgac accggtaccg gattgcggcg agaaaaccta
    10081 tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg attccggaat
    10141 aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga tcagttatct
    10201 tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat gcggatgcaa
    10261 agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc tggttcacga
    10321 agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga aacaggttgc
    10381 cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg ccattaacgt
    10441 tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag gtgcaagtat
    10501 catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg actatgcggc
    10561 tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg cggagaaagg
    10621 tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa tttccggcgg
    10681 acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc gtgcggggca
    10741 accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga gctacgtcac
    10801 cgcagaagtg cacggtgtgt gcggcggcga gcatttaggt taaaaaaatg cccggttgtg
    10861 aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa acgccttatc
    10921 cgcctacaaa cgcatgaaga ttcaacatgt tgcaatagat tttgtaggcc tgataagcgt
    10981 agcgcatcag gcattgagca cctaatgccg gatgcggcgt gaacgcctta tccggcctac
    11041 aaacgcacga agattcaaca tgttgcaata gatattgtag gcctgatgag cgtagcgcat
    11101 caggcaattc tactttactt cgctttggcg gtttcttcgc cgaccagacc tatcttcagg
    11161 taacccgcct gatgcagcgt atccattacc ttcatcaacg tctcgtaatc gacggtttta
    11221 tccgctcgga agaagatggt ggtgtctttc ttgccttcgg ttaacgcatt caacgccgta
    11281 atcattgttt catcggtgac cggatcgtta ccgataaaca tcgagttgtc tgccttcacc
    11341 gacagataaa ccggtttttc cgggcgcggc tgcggcgtgc tggtagaagc aggcaagttc
    11401 accttcacat ctaccgtcgc taacggtgcc gccaccataa agataatcag cagaaccaac
    11461 atcacgtcga taaacggcgt cacgttgata tcatgcattt cgccgttatc gtcgaggttt
    11521 tcgttaagat gcattgccat cggatattat cctgcgcgta atttttgtgc gacacgaacc
    11581 ggatgcgcag cggcgctggc ttccagatcc aggtcacggc tttgcagcaa caatacctgc
    11641 gctgcaacat cacccagcat cgctttaaag ccgccaatct ggcgtgcaaa tacgttatag
    11701 ataacgaccg caggaatcgc tgccacgagg ccgattgccg ttgctaacag agcttctgcg
    11761 atacccggcg caacgactgc caggttagtg gtctgcgttt gcgcgatacc aataaagctg
    11821 ttcatgatgc cccagaccgt accaaacaga ccaacgaacg gagaaatcgc gccgatggtt
    11881 gccagatagc cgttaccgcg gcccatttga cgacccactg cggcgacctg acgctccaga
    11941 cggaagctgg tacgttcttt aataccttcg ttatcgtcgc tgccttctga cagttccagc
    12001 tcattctgcg cttcattgag caaatgcagg cttaagcttt tgctaccaaa atcagcggcg
    12061 atatcgttgg cctggtttaa ggaacgcgct tcagccagca gttgctgctc gcgcttaagg
    12121 cgacgcttct gattgaagaa ctctacgctc ttactgaaga agattgccca agtgactacg
    12181 gaggccaaaa taagcccaat catcacgcac ttaacgacaa tatcggcgtg ctgatacata
    12241 ccccagacgg aaaggtccgt ctgcattaaa ttattaccca ctgtgtatct ccaggacgca
    12301 agtcacaaaa tctgcgcata ataatatcaa aatgacgtcg aattgatagt cgttctcatt
    12361 actatttgca tactgccgta cctttgcttt cttttccttg cgtttacgca gtaaaaaagt
    12421 caccagcacg ccatttgcga aaattttctg ctttatgcca attcttcagg atgcgcccgc
    12481 gaatattcat gctagtttag acatccagac gtataaaaac aggaatcccg acatggcgga
    12541 caaaaagctt gatactcaac tggtgaatgc aggacgcagc aaaaaataca ctctcggcgc
    12601 ggtaaatagc gtgattcagc gcgcttcttc gctggtcttt gacagtgtgg aagccaaaaa
    12661 gcacgcgacg cgcaatcgcg ccaatggcga gttgttctat ggacggcgcg gaacgttaac
    12721 ccatttctcc ttacaacaag cgatgtgtga actggaaggt ggcgcaggct gcgcgctatt
    12781 tccctgcggg gcggcagcgg ttgctaattc cattcttgct tttgtcgaac agggcgatca
    12841 tgtgttgatg accaacaccg cctatgaacc gagtcaggat ttctgtagca aaatcctcag
    12901 caaactgggc gtaacgacat cgtggtttga tccgctgatt ggtgccgata tcgttaagca
    12961 tctgcagcca aacactaaaa tcgtgtttct ggaatcgcca ggctccatca ccatggaagt
    13021 ccacgacgtt ccggcgattg ttgccgccgt acgcagtgtg gtgccggatg ccatcattat
    13081 gatcgacaac acctgggcag ccggtgtgct gtttaaggcg ctggattttg gcatcgatgt
    13141 ttctattcaa gccgccacca aatatctggt tgggcattca gatgcgatga ttggcactgc
    13201 cgtgtgcaat gcccgttgct gggagcagct acgggaaaac gcctatctga tgggccagat
    13261 ggtcgatgcc gataccgcct atataaccag ccgtggcctg cgcacattag gtgtgcgttt
    13321 gcgtcaacat catgaaagca gtctgaaagt ggctgaatgg ctggcagaac atccgcaagt
    13381 tgcgcgagtt aaccaccctg ctctgcctgg cagtaaaggc cacgaattct ggaaacgaga
    13441 ctttacaggc agcagcgggc tattttcctt tgtgcttaag aaaaaactca ataatgaaga
    13501 gctggcgaac tatctggata acttcagttt attcagcatg gcctactcgt ggggcgggta
    13561 tgaatccttg atcctggcga atcaaccaga acatatcgcc gccattcgcc cacaaggcga
    13621 gatcgatttt agcgggacct tgattcgcct gcatattggt ctggaagatg tcgacgatct
    13681 gattgccgat ctggacgccg gttttgcgcg aattgtataa cattgccact tttggacaat
    13741 tttgcagaca ttttattgtg aaaagtctta aattgttgcg tccgggatca aggcgtcccg
    13801 gacgaatcag gagtaaaata ggcagataaa ggcttaaacg ctgttccaca ggaaagtcca
    13861 tggctgttat tcaagatatc atcgctgcgc tctggcaaca cgactttgcc gcgctggcgg
    13921 atcctcatat tgttagcgtt gtttactttg tcatgtttgc cacgctgttt ttagaaaacg
    13981 gcctgctgcc cgcctcattt ttgccaggcg acagcttgtt gatactggca ggcgcattga
    14041 ttgcccaagg ggttatggat tttctgccta cgattgcgat tctgaccgcc gcagcaagtc
    14101 tgggctgctg gctaagttat attcaggggc gctggttagg gaataccaaa acggtgaaag
    14161 gctggctggc acagcttcct gctaaatatc accagcgcgc cacctgcatg tttgaccgcc
    14221 acggtctgct ggcgctgctg gctggacgtt ttcttgcatt tgtccgtacg ctgctgccaa
    14281 ccatggcggg aatttccggt ctgccaaacc gccgcttcca gtttttcaac tggttaagtg
    14341 gattgctgtg ggtcagcgtg gtaaccagtt ttggctatgc cttaagtatg attccgttcg
    14401 ttaaacgcca tgaagatcag gtaatgacgt tcctgatgat cctgccaatt gccttgttaa
    14461 ccgctggctt gttaggcacg ctgtttgtgg tgattaaaaa aaaatactgt aacgcctgac
    14521 gattttcccc gttcccggct gctgtaccgg gaacgtattt aattcccctg catcgcccgc
    14581 attcttgccg catcttcccc cggcgtcaca ccgaagtaac gtttaaactc acggctaaat
    14641 tgcgatgcgc tttcatagcc gacacgcatc gctgctgcgc tggccttcat gccgtcatgg
    14701 atgatcatca tccgcgcctt atgcagacgg taattcttca aatactgcaa cggcgaggtg
    14761 ctggtgacag acttaaaatt atggtggaac gccgatacgc tcatgttggc ttctgccgcc
    14821 agttgctcga cgctcaggtt ttcggtgtat ttattctcaa tccgtttcag cacgcggcta
    14881 atcagactga agtgagtctg gcgactgacc agcgccagta acgcgccgcc gcaaggtccg
    14941 gtcagcacgt agtacagaat ttcgcggatg atctgtttgc cgagaatacg cgcatccagt
    15001 ggtcgctcca tcacgtcgag taaccgctcc gccgcgcata aaatctcttc tgataacgtg
    15061 gcggagttaa tcccgctggc tgccatcgac ggctggaaat gctcatcttc gccaatgtcc
    15121 atcaacagtt cctgtaactg caaaatatcg acattgagac gcaaccctgc cagcggcacc
    15181 tctgacgtgg cataggtttc gcactcaaac ggcaacggca ccgtcagcag caggtattca
    15241 ttggcatcat aacgaaacac gcgttcattg atataaccga ttttatgccc ggaaaagaga
    15301 attatgatgc caggctcgta catcaccgga gtacgtgcga aaggcgtctc gccatacaac
    15361 aaacgcacat cgggcaacag ttctgacaaa ctattttctt taattttcag tttattaact
    15421 ttatccgcca gcaagcggca aatctcttca cgtttcatat cgcgtaattt cttaggaata
    15481 atgcggcaat ttgattgtgc gcaattttgt agcatttctc cagcactctg gaggaatagg
    15541 caagatattg gcagaaatga gcattgagag ccaaggcgct ggcgatcaca atgaaaaaca
    15601 tcaggcagat cgttctctgc cctcatattg gcccagcaaa gggagcaagt aatgaacaac
    15661 tttaatctgc acaccccaac ccgcattctg tttggtaaag gcgcaatcgc tggtttacgc
    15721 gaacaaattc ctcacgatgc tcgcgtattg attacctacg gtggcggcag cgtgaaaaaa
    15781 accggcgttc tcgatcaagt tctggatgcc ctgaaaggca tggacgtgct ggaatttggc
    15841 ggtattgagc caaacccggc ttatgaaacg ctgatgaacg ccgtgaaact ggttcgcgaa
    15901 cagaaagtga ctttcctgct ggcggttggc ggcggttctg tactggacgg caccaaattt
    15961 atcgccgcag cggctaacta tccggaaaat atcgatccgt ggcacattct gcaaacgggc
    16021 ggtaaagaga ttaaaagcgc catcccgatg ggctgtgtgc tgacgctgcc agcaaccggt
    16081 tcagaatcca acgcaggcgc ggtgatctcc cgtaaaacca caggcgacaa gcaggcgttc
    16141 cattctgccc atgttcagcc ggtatttgcc gtgctcgatc cggtttatac ctacaccctg
    16201 ccgccgcgtc aggtggctaa cggcgtagtg gacgcctttg tacacaccgt ggaacagtat
    16261 gttaccaaac cggttgatgc caaaattcag gaccgtttcg cagaaggcat tttgctgacg
    16321 ctaatcgaag atggtccgaa agccctgaaa gagccagaaa actacgatgt gcgcgccaac
    16381 gtcatgtggg cggcgactca ggcgctgaac ggtttgattg gcgctggcgt accgcaggac
    16441 tgggcaacgc atatgctggg ccacgaactg actgcgatgc acggtctgga tcacgcgcaa
    16501 acactggcta tcgtcctgcc tgcactgtgg aatgaaaaac gcgagaccaa gcgcgctaag
    16561 ctgctgcaat atgctgaacg cgtctggaac atcactgaag gttccgatga tgagcgtatt
    16621 gacgccgcga ttgccgcaac ccgcaatttc tttgagcaat taggcgtgcc gacccacctc
    16681 tccgactacg gtctggacgg cagctccatc ccggctttgc tgaaaaaact ggaagagcac
    16741 ggcatgaccc aactgggcga aaatcatgac attacgttgg atgtcagccg ccgtatatac
    16801 gaagccgccc gctaagcttt ttacgcctca aactttcgtt ttcgggcatt tcgtccagac
    16861 ttaagttcac aacacctcac cggagcctgc tccggtgagt tcatataaag gaggaacgta
    16921 tggctaatcc aaccgttatt aagctacagg atggcaatgt catgccccag ctgggactgg
    16981 gcgtctggca agcaagtaat gaggaagtaa tcaccgccat tcaaaaagcg ttagaagtgg
    17041 gttatcgctc gattgatacc gccgcggcct acaagaacga agaaggtgtc ggcaaagccc
    17101 tgaaaaatgc ctcagtcaac agagaagaac tgttcatcac cactaagctg tggaacgacg
    17161 accacaagcg cccccgcgaa gccctgctcg acagcctgaa aaaactccag cttgattata
    17221 ttgacctcta cttaatgcac tggcccgttc ccgctatcga ccattatgtc gaagcatgga
    17281 aaggcatgat cgaattgcaa aaagagggat taatcaaaag catcggcgtg tgcaacttcc
    17341 agatccatca cctgcaacgc ctgattgatg aaactggcgt gacgcctgtg ataaaccaga
    17401 tcgaacttca tccgctgatg caacaacgcc agctacacgc ctggaacgcg acacacaaaa
    17461 tccagaccga atcctggagc ccattagcgc aaggagggaa aggcgttttc gatcagaaag
    17521 tcattcgcga tctggcagat aaatacggca aaaccccggc gcagattgtt atccgctggc
    17581 atctggatag cggcctggtg gtgatcccga aatcggtcac accttcacgt attgccgaaa
    17641 actttgatgt ctgggatttc cgtctcgaca aagacgaact cggcgaaatt gcaaaactcg
    17701 atcagggcaa gcgtctcggt cccgatcctg accagttcgg cggctaacat gcaaattctc
    17761 ccggtggcgg taatgttccg ctaccggact tttcagaaat catttattcc cctcgcgtcc
    17821 cgcccgttgt tactcttcct tgttcaggaa tgccaaatat aaggacatca tcatgcagag
    17881 ccggaagctc ttaaaagaac aactcatcta tatccgggat aaacgcaacg gagaggtgaa
    17941 aaacagatga aaataatact tctgttttta gcagccctgg caagttttac cgtacacgca
    18001 cagcccccct cactgaccgt agaacaaaca gtccggcata tttatcagaa ctataaatca
    18061 gatgccactg ccccttattt tggtgaaacc ggagagcggg cgataacttc tgcgcgtatt
    18121 caacaggcgc ttaccctgaa cgacaatctt acgctgccgg gcaatattgg ctggctggat
    18181 tatgatccgg tttgtgattg tcaggatttt ggcgatctgg tgctagaaag cgttgcgata
    18241 acccaaactg acgccgatca tgccgatgcc gttgtgcgct ttcgtatctt taaagatgat
    18301 aaagaaaaga ccacgcagac actgaaaatg gtggcggaaa atggtcgttg ggtcattgac
    18361 gatattgtca gcaatcatgg cagcgtctta caagcagtta atagcgagaa tgaaaaaacg
    18421 ctggccgctt tagcttcgtt gcaaaaagaa cagccggaag cctttgttgc cgaactcttt
    18481 gaacatattg ctgattatag ctggccgtgg acgtgggtgg tttccgactc ttaccgccag
    18541 gcggttaatg ccttctataa aaccaccttc aagacggcca ataatcccga tgaagatatg
    18601 caaatagaac ggcaatttat ttacgacaat ccgatctgtt ttggcgaaga gtcgctattt
    18661 tcacgcgttg atgaaattcg agtcctggag aaaaccgccg attccgcccg cattcatgtt
    18721 cgttttacgc tgaccaatgg caacaacgaa gagcaagaac tggttttaca gcggcgcgaa
    18781 ggcaagtggg aaatcgctga ttttatccgc ccgaacagcg gcagcctact taagcagatt
    18841 gaggcaaaaa ccgccgccag attaaagcaa tgagctgaat taaataacaa ttagccggaa
    18901 caataaataa aagggaacac tatatgaaaa cgattttcac cgtgggagct gttgttctgg
    18961 caacctgctt gctcagtggc tgcgtcaatg agcaaaaggt caatcagctg gcgagcaatg
    19021 tgcaaacatt aaatgccaaa atcgcccggc ttgagcagga tatgaaagca ctacgcccac
    19081 aaatctatgc tgccaaatcc gaagctaaca gagccaatac gcgtcttgat gctcaggact
    19141 attttgattg cctgcgctgc ttgcgtatgt acgcagaatg ataaaaaaat ccccggcagc
    19201 atgtcagttg ccggggattt tttttaacgt ccaaccgccg ctttagggcg tttcttcgca
    19261 ccagcattca ccggacgaga ttgcgtagac gacgcttttt ttgccgtagc aggcgtctga
    19321 cgctgggtcg ccatcggcgt atgtttcgtc aacgccggac gggtattgcg gttctggcga
    19381 cgagcttcac gcatctcttc aatggttggc gcaggcacta agcaatcgcg acggctgcca
    19441 atcagatgct ttttgcccat cgcttccagc gcctggcgga ttaacggcca gtttgccgga
    19501 tcgtggtaac gcaacaacgc tttatgcaaa cgacgctgtt tgtcgccctt cggtacgaag
    19561 acgtcttcgc tcttataacc aatcttcgcc agcgggtttt tcccggtgta atacatggtg
    19621 gttgagttcg ccagcggcga tgggtagaag ttctgtacct ggtcgagacg gaaacgatgc
    19681 tttttcagcc acagcgccag attcaccata tcttcatcac gcgtaccggg gtgcgcggag
    19741 atgaaatagg ggatcagata ctgctcttta cctgcctgtt ttgagtaagt atcgaacagc
    19801 tctttaaagc ggtcatagct gcccatgccc ggcttcatca tcttcgataa cggcccttct
    19861 tcggtatgtt ccggggcaat cttcagataa ccgccgacgt gatgggtcgc caactctttg
    19921 atatagcgcg gatcttccac ggcgatgtca taacgtacgc ctgaggcgat gaggattttt
    19981 ttaatgcctt tcagatcacg cgcacggcga tagaggttga tcgtcggttc gtggttagtg
    20041 tccatgtgcg gacaaatatc cggataaacg cacgacaaac ggcgacaagt ttgttcagcg
    20101 cgtggcgatt tgcagcgcaa catatacatg ttggcagttg gcccaccgag atcggaaatc
    20161 acgcccgtaa aacctggaac ggtgtcgcgg atcgcttcga tctcattaat gatcgaatct
    20221 tccgaacggc tctgaataat gcgcccttcg tgctcggtaa tagaacagaa agaacagccg
    20281 ccaaagcagc cgcgcataat gttgaccgaa aaacggatca tttcgtaagc cggaatacgg
    20341 gcattgccat aggccggatg tggcacgcgc ttgtacggca gcgcaaaaac gctgtccatc
    20401 tcttcggtag aaagcgggat agcaggcggg ttgatccaca catagcggtc accgtgtttt
    20461 tgcatcaatg cgcgggcgca gcctgggttg gtttcgtggt gcagaatgcg cgaagcatgg
    20521 gcgtacagca ctttatcgcc cttcactttc tcgaaagaag gcagcaacac gtaggttttt
    20581 tcccacggtt tcgggcgcgg tggctgcacg gttacggctt tggcttcctg ctttttcggt
    20641 gccaccggtt tgttatccgc gcacggcaaa tcttcaccat acggatgcgg gattgggtcg
    20701 atttttccag gggtatcaag acgggtggaa tccacgccgc tccagccagg cagcgcctct
    20761 ttcacgataa tcgcggtatt acgcacatcg cggatttcac taatcggctc gcccatcgcc
    20821 agacggtgcg ccacctccac cagcggacgc tcaccgttac caaacatcag catgtcggct
    20881 ttcgaatcca ccagcacgga acggcgcacg gtatcggacc agtaatcata atgtgcggta
    20941 cggcgcagac tagcctcaat accgccgagg atcaccggta catctttcca cgcctcttta
    21001 caacgctggg tataaaccag tgtggcgcga tccgggcgct tacccgcgac gttatccggc
    21061 gtgtaggcat cgtcatgacg taaacggcga tcggcggtat aacggttgat catcgaatcc
    21121 atgttgccag cagtaacacc gaaaaacaga ttcggtttac ccagacgcat gaaatcgtct
    21181 ttgctgctcc agtctggctg ggcgatgatc ccgacgcgaa agccctgtgc ttccagcata
    21241 cgaccgcaaa tcgccatccc gaagcttggg tgatcgacat acgcgtcgcc agtaaccaaa
    21301 atgatgtcgc agctatccca gccaagttga tccatctctt cacgagacat cggcaaaaac
    21361 ggtgccggtc caaaacaggc ggcccagtac tgcggccagg agaacaggtc gcgatccggt
    21421 tggatcaggg agatagagct cattttgctt ccagaaatga taaaaaaata atcaaaggcc
    21481 ggggattata agccggaacg aaagagaaat cgaaaagtat tccatactcg ccctcctcgg
    21541 gcgagtatga agattacggt accggattga ctaacagttg cccaatcgag ccacggtccg
    21601 ccatttccag cgtctgactg ttgaagtaga acgggaagtg cgcccaggaa ggctgaccga
    21661 aataaacaag cagctccact tgtccgtcaa cccaaacggt atctttccag cctctgtctt
    21721 ccgggaacgg catcgcgcca ttcacgttac ggatctggaa cattacgcct tcaatatgga
    21781 acgcttgcgg ctcgtccgcg cgtaccgtcc agcgttccca cgttccttgc tgcgcggtga
    21841 catcaatacg gttgacgtcc cacagctgtc cattaatacc cgggtcatca cccagactga
    21901 tatcgcgact gcgaattggc gaaccagcca tgatttcagt tggcagcaag cgcatcggaa
    21961 gactgtctgt gaccagcggc agaaggccgg ttgggcgcag cgttagcacc agggtagaaa
    22021 ccagaatact ggatggctca aagaagccgc gaatacgatc aacaatgctc gccgcttcgc
    22081 cacaggtgat cgacacttca tcgccgttgc tcatatccac cagaatctcg cggcgctcgc
    22141 ccggtgccag cgaaagttgc ttcaccgata caggagcagg caggaatccc tgatcgccag
    22201 aaatcacatg taacgggcga ccatcgctca tctgtagttg atagcgacga gagttcgacg
    22261 cgttcagcag tcgcaagcgc acccagccac gcgagacttc aacgtacggg ctttgtacac
    22321 cgttaaccag cagcgtatca ccaacaaagc cgccgcttcc cggttcgttg tattctggcg
    22381 taccaaagtt atccagccgt ttatcctgga taatgaccgg aaaatcatcc acaccataat
    22441 ggttggggat aggcagcgac ttgctgactt catcttccac cagccacatt ccggcaaggc
    22501 cgttatagac ctgctgagcc gtgcggttgg gagtattggc gtgataccac agagtagctg
    22561 cgttctggcg aatgggcagt acgggtgccc agtcagcgtt tggcgacatc atccgtgccg
    22621 gaccgcccat cagcgggcct ggtacctgta gcccggccac cgtcattgag acattttctg
    22681 tcaggcggtt gctgtaaata agcttaacat cgtcgccctt ccagacgcgg atagtcggcc
    22741 ccaggtaacg accattgatt ccccagaccg acgcgcgtgt ccctggcgta aatgaccagt
    22801 gcacacgttg tacagtcata aacagcggtt gcccacggcg agattcaagt agcggcggaa
    22861 cgggtagcgg ttgctgttgc ccggctgcgc tggccttcag gggaacagcg cctgcacaaa
    22921 gtgcaatccc cgatgcctga atgaactgac gccgactgag tgacatattt gctccatgta
    22981 aaactggcta aagtaatacc gtgcaggaaa gtgttccccg caaaaataca gattgccgac
    23041 ttaaactttt ccggcggctt cgcgttctgc gacttcttta tcgagctcgg cgattttttg
    23101 ttccattatc gaacgacaat gggcagccag ctcacgaacc tgatctttgc catactgact
    23161 gacgtcaatt ggcggcagca tttcgacaat caccagaccg ttgtgcagtc gattaagatt
    23221 aatcttattc gaagttgtag agacgcacac gggaataatc gggacgcccg ccgcaattgc
    23281 cgcgtgaaat gctccagtct tgaaaggtag caggccgcga ccacggctgc gggttccttc
    23341 cgggaacatc cagatggaaa tacggcgttt tttgaaatga ttcacgactt ccgcaatggt
    23401 gccgtgagct ttagtgcgat tgtttctgtc gatcaataag ttgccggtta accagtacaa
    23461 ctgcccgaag aaggggatcc acagcaagct ctttttacct accgtcaccg tcggcggttg
    23521 cacaatgttc gatgctgtca ccatgtcata gttgttctgg tggttagcga tatagatagc
    23581 attgccgtag ctttcagcat ctgcaggttt gcggcactca actttcaggc cgaacagcgg
    23641 cgcaagacgg ccaaacatat gcccaaaggt ggccacatgt ttcgggttac gcgggctgaa
    23701 aaggcagtaa atggagccga atacacagac taagatgctg taaatcacgg taataataag
    23761 acgaaagata tatagcataa caccctctga aggtctgaga gactggcatt gtacgtcacc
    23821 tgaaatcagg ttagggcatt gttaaaagcc gctctctgga aaagcgtatt gttaatcgca
    23881 acgcatgaat aaacaacggt ttcaagggaa tttttatcgt ttccgggggt tgaatacgct
    23941 gccggatgac gacttaacgt ttcatccggc gttccttgca agcgggagga aacaggccct
    24001 ccccggcata ttactcttcg ctatcaccgc tgctggcacg gcgaggagag tcgatctcaa
    24061 cacgatcgat acgctgcaaa ccgcgcatca acgtaccgcg gcgtccacgt tcgccagtga
    24121 ctttctgtaa ctcttccggg cgcagtttaa ttttgcgttt cccaacatga atggtcagcg
    24181 tgctttgcgg cggcagaacg tacaattgcg ccagaccatc ttctccacgc gcggcttctg
    24241 ccgatggaat gttgataatc ttgttgcctt tgcccttcga cagctgcggc agatcgctta
    24301 ccgggaacat caacatacgg cctgcctgag tgattgccag cagcatatcg gaagcatctt
    24361 caatcaccac cggcggcata acatgggcat tttccggtaa ggtgatcaaa gccttacctg
    24421 cacggttacg cgccaccaga tcgttaaagg tgcagacgaa accgtaaccc gcatcggaag
    24481 ccatcagcag tttctgatcg tcgctttcca tcagcatatg gtcaacggtc gccccaggcg
    24541 gcaacgttaa tttgccggtg agtggttcgc cctgaccacg cgccgacggc agcgtaatcg
    24601 ggtcgatggc atagctacga ccggtggaat caacaaacac taccggttgg ttgctcttac
    24661 ctttcaccgc cgctttgaag ctatcacccg ctttataatt caggcccggc gcgtcgatat
    24721 catggccttt agcgctgcgt acccagccca tctgcgacag cacaatggtg acaggttcag
    24781 acggcagcat gtcgtgctcg ctcatcgctt tcgcttcttc gcgttcctgc aacggcgaac
    24841 gacgatcgtc accgtaggct tgcgcgtctg cctgcagttc tttcttcagc aggttattca
    24901 ttttacgctc ggaagccaaa atgccctgca actggtcgcg ctctttttcc agctcactct
    24961 gctcaccgcg aatcttcatc tcttccagtt tggcaagatg acgcagtttc agttcgagga
    25021 tcgcttccgc ctgggtttcc gtaaggccaa accgcgacat cagcgccggt ttcggttcat
    25081 cttcgttacg aatgatctca atcacttcgt cgatgttgag aaacgccacc agcaaacctt
    25141 ccaggatatg caggcgcttg aggactttct ccagacgata gttcagtcgg cggcgcacgg
    25201 tatcgcgacg gaacaccagc cattcggaga ggatttccag caggtttttc accgccggac
    25261 gaccatccag accgatcata ttgaggttaa tgcgatagct cttttccaga tcggtggtag
    25321 cgaagaggtg gttcatcacc tgatccatat ccacgcggtt ggaacgcggc acaatcacca
    25381 gacgggtcgg gttctcgtgg tcagattcat cgcgcagatc gtcaaccatc ggcagctttt
    25441 tgttgcgcat ttgcgcagca atttgctcca gtacgcgcgc acctgaaacc tgatgcggca
    25501 atgcgctgat aaccaccgcg ccatcttctt tcttccacac cgcgcgcata cgcactgaac
    25561 cacgtccgtt ctcgtagatt ttacggatct cggcgcgcga agtgataatt tccgcttcag
    25621 tcggataatc cggcccctgc acgatatcca gcagctgatc gagcgtggtt ttcggctggt
    25681 cgattaatgc gattgccgcc tgagccactt cacgcaggtt atgcggtgga atatcggtcg
    25741 ccatgccgac ggcaataccg gtggtgccgt taagcaaaat gtttggcaga cgggcgggta
    25801 gcattttcgg ctcctgcaaa gtgccgtcga agtttggcac ccagtcagcc gtcccctgcc
    25861 ccagctcgct caatagcagc tcggaatatt tcgacaaccg ggattcggtg taacgcattg
    25921 ccgcgaacga tttcggatcg tccggcgcgc cccagttccc ctgaccatca accagcggat
    25981 aacggtaaga gaacggctgc gccatcagga ccatcgcttc ataacaggcg ctatcgccgt
    26041 gcggatggta tttacccagt acgtcaccga cggtacgggc cgatttttta aatttggcgc
    26101 tggcattcag gcccagttca gacatcgcat acacaatgcg gcgctgaaca ggtttaagac
    26161 catcgccaat aaacggcaac gcacggtcca tgatcacgta catggagtag tttaagtagg
    26221 cgttttccgt aaattcatgt agcgcaaggc gctctgccat atcgctcatt aattctgatt
    26281 cctcaactta ttcgccagcc ttcaaagggg caatattgcc ggagatacta cattatctgc
    26341 cgcgtcgagt cacaggagat cagcgtgaat agcgccgccg gacgtgctcc gacggcgata
    26401 aaggcatcaa tgtgccttga tatacaactc tttgctgtag acttgatcgg catagagggc
    26461 agcacaaggt tgcgcccatg ccacaccact cggcatgggg gtccgctccg ggcgattcga
    26521 gaagttgagg ttatgcgagt ccagggaagc ctaaagcatc aaagtagtcg actgtaagta
    26581 tatggttcag cccagtttag ctgtgtctcc accagtgccc cgcgcctcct gccataagtg
    26641 cggcttgctc atgggtaccc ccgagacggc ttacggcgca ccggataata aatttcgcgt
    26701 atacatggta ttccttaccg gagaaattat aagcccaaca acaatacgcc tgtggcattg
    26761 ttcgctcaaa cttatcgcga gtgatttggc tcacactccg gtaaagattc cccatcacgc
    26821 caacggcaca taaacatccg tttccagtcg ctgctctgtc accccttcgg caagattggt
    26881 gtagtgaaac agaatcggtg ctttacgcat tttctcgccg cttgcaggca gccagtggcg
    26941 aataatgccc catatcgtgt gtgaaatatc gtccagctcg ccaacgtggc gggccacggc
    27001 ataacgtcca ccggtaagtt caccattgct cacaccataa cgattatcgg gaatcggttc
    27061 gctaacgctg ccgcagatat caaagcgaaa cgcttccggt gcggtggtgg cagggtcgtc
    27121 ccaggcgacg ccaaaagtct ggctttggtt aacaggagaa agccccgtct ctttacgcca
    27181 catgataaat ttcgccacac tgtagttgag caactcaggg ctacagcggt gcggcaacat
    27241 cgccacaggg attgatggga aatcgataat gtttacatcc agtgtcaggt ttgtcatttt
    27301 gcctccctgc gaaattattc ccgggaacgc gtcccgggag cggtaacaat tacggattta
    27361 ctttgcggat ctgtttgacg tcaatttcaa cagaattcca gtctttatcg acttcaccct
    27421 gaatctcaac cgtatctttc ggcgtcaccg tcacaccgtt ccagcgtttg tggtcgatat
    27481 caacattgat agtaccgctg gcatctttaa acacgtagag atcgtcagag atgcgttcaa
    27541 cgatattgcc gcgcagggtt acccaggtgt cgtcacgcag ggattttgcg ctttctacgg
    27601 tcgttacgct gccgttcggc ccctggaatc ctccggcctg actttgcgtt gccgatgggc
    27661 cagaaaaacc gccctgctct gctgccatca ccggtgcgct gcacagggcc attactgcga
    27721 ttactgctgc gaattttttc atgtttatta ctccctttaa tgtctgtttc cgagcattta
    27781 acaagatagt ccttaacaac ttcttaaggg aaaaaaataa aatttagtgc tgtacagagc
    27841 gcgttacaac acggtttact ggcagcaaat acggttatcg cagggatgaa aaaatgcgaa
    27901 ttttactgat agaagatgac atgctgattg gcgacggcat caaaacgggc cttagtaaaa
    27961 tgggttttag cgtcgactgg tttacacaag gtcgtcaggg aaaagaggcg ctttatagcg
    28021 cgccttatga tgcggtgatc ctggatttaa ccttaccagg catggatggt cgcgatattt
    28081 tgcgcgaatg gcgagaaaaa ggtcagcgtg agccggtact gatcctgacc gcgcgcgatg
    28141 cgctggagga acgtgtagaa gggctgcgtc tgggagctga cgattatctg tgtaaacctt
    28201 ttgcgttgat agaagtcgcc gccaggctgg aagctctgat gcgccgaacc aacggccagg
    28261 ccagcaacga gctgcgccac ggtaacgtca tgctcgaccc cggcaaacgt atcgccacgc
    28321 tggctggcga acccttaaca ctgaaaccaa aagaatttgc cctgctggaa ttactgatgc
    28381 gtaacgctgg tcgggtactg ccgcgcaaac tgattgaaga gaaactgtat acctgggacg
    28441 aagaggtcac cagtaatgcc gttgaagtgc atgtgcatca tctgcgacgc aaactcggta
    28501 gtgattttat tcgtaccgtg catggtattg gctacacatt aggtgagaaa tgaaatttac
    28561 ccaacgtctt agtctgcgcg tcaggctgac gctaatcttt ttaattctgg cctcggtgac
    28621 ctggctgctt tccagctttg tcgcctggaa acaaacaacg gataacgtcg atgaattgtt
    28681 cgacacccaa ctgatgctgt ttgccaagcg gttaagtacg ctcgatctca acgaaatcaa
    28741 cgcggcggat cgcatggcac agacgccaaa taaattaaaa cacggtcatg ttgatgacga
    28801 tgcgctgacc tttgccatct ttacccacga cggcagaatg gtccttaacg atggcgataa
    28861 cggagaagat attccttata gctatcaacg ggaaggtttt gctgacgggc aactggtggg
    28921 cgataaagat caatggcgtt ttgtctggat gacctcacct gatggcaaat atcgcatcgt
    28981 tgttggtcag gaatgggaat accgtgaaga catggcgctg gcgattgttg ccgggcaatt
    29041 gatcccgtgg ctggtcgcac tgccgattat gttaatcatc atgatggtac tattgggtcg
    29101 tgaactcgcg ccgctgaaca aactggcgct ggcgctgcgt atgcgtgacc ctgactcgga
    29161 aaaaccacta aacgcgactg gcgtacccag cgaagtgcgt ccgttggttg agtcgctaaa
    29221 tcaactgttc gcccgcacac atgcgatgat ggttcgtgaa cgacgcttta cctccgacgc
    29281 agctcacgaa ctacgtagcc cgttaacggc gctgaaagtg caaaccgaag ttgcgcagct
    29341 ttctgacgat gatccgcaag cgcggaaaaa agcgctgctt caattacatt acgggatcga
    29401 tcgcgctact cgtctggttg atcaactgct cactctatcg cggctggact cactggataa
    29461 ccttcaggac gtcgcggaga tcccgcttga agatctcctg caatcgtcgg tgatggatat
    29521 ttaccacacg gcgcagcagg cgaaaattga cgtgcgactg acactcaatg cccacggcat
    29581 caaacgcacc ggacaaccgc tattgctaag tttgttggtg cgaaatttgc tggataacgc
    29641 cgtgcgctac agtccacagg gcagcgtggt agacgtcacg ctgaatgctg ataatttcat
    29701 cgtgagggat aatggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt
    29761 ctatcgccca cccggacaaa ccgctaccgg cagcgggctt gggctatcga ttgtccagcg
    29821 aatcgccaaa ttgcatggca tgaatgttga atttgggaat gcggaacaag gtggatttga
    29881 ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac tctcatagat
    29941 tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa accagacaaa
    30001 taccgagcga gaatacatat acccaaaact cgaacatttc ccgcataaag agttttctta
    30061 agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct tgcaatatac
    30121 gatcttcgcc tgaaacggaa ggtatactgc aaatattgtc tcccgactct agcatccagt
    30181 catacttatt gaaagcaaaa aaagcatcta ttaaccaata agtaagcatt atgtataaca
    30241 gagc
//
LOCUS       DAZKIV010000041        29795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZKIV010000041 DAZKIV010000000
VERSION     DAZKIV010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.041"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(209..2782)
                     /gene="clpB"
                     /locus_tag="ACHOT6_001849"
     CDS             complement(209..2782)
                     /gene="clpB"
                     /locus_tag="ACHOT6_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFP9947079.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2912..3643)
                     /gene="yfiH"
                     /locus_tag="ACHOT6_001850"
     CDS             complement(2912..3643)
                     /gene="yfiH"
                     /locus_tag="ACHOT6_001850"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFP9947080.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3640..4620)
                     /gene="rluD"
                     /locus_tag="ACHOT6_001851"
     CDS             complement(3640..4620)
                     /gene="rluD"
                     /locus_tag="ACHOT6_001851"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFP9947081.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHTPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4755..5492
                     /gene="bamD"
                     /locus_tag="ACHOT6_001852"
     CDS             4755..5492
                     /gene="bamD"
                     /locus_tag="ACHOT6_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFP9947082.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5763..6104
                     /gene="raiA"
                     /locus_tag="ACHOT6_001853"
     CDS             5763..6104
                     /gene="raiA"
                     /locus_tag="ACHOT6_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFP9947083.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6207..6332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6208..6255
                     /gene="pheL"
                     /locus_tag="ACHOT6_001854"
     CDS             6208..6255
                     /gene="pheL"
                     /locus_tag="ACHOT6_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFP9947084.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6354..7514
                     /gene="pheA"
                     /locus_tag="ACHOT6_001855"
     CDS             6354..7514
                     /gene="pheA"
                     /locus_tag="ACHOT6_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFP9947085.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7557..8678)
                     /gene="tyrA"
                     /locus_tag="ACHOT6_001856"
     CDS             complement(7557..8678)
                     /gene="tyrA"
                     /locus_tag="ACHOT6_001856"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFP9947086.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8689..9759)
                     /gene="aroF"
                     /locus_tag="ACHOT6_001857"
     CDS             complement(8689..9759)
                     /gene="aroF"
                     /locus_tag="ACHOT6_001857"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFP9947087.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRRTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            9969..10334
                     /gene="yfiL"
                     /locus_tag="ACHOT6_001858"
     CDS             9969..10334
                     /gene="yfiL"
                     /locus_tag="ACHOT6_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFP9947088.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKAADENASTIRLN"
     gene            10484..11002
                     /gene="yfiR"
                     /locus_tag="ACHOT6_001859"
     CDS             10484..11002
                     /gene="yfiR"
                     /locus_tag="ACHOT6_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFP9947089.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10992..12143
                     /gene="dgcN"
                     /locus_tag="ACHOT6_001860"
     CDS             10992..12143
                     /gene="dgcN"
                     /locus_tag="ACHOT6_001860"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFP9947090.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALAALGQQGQFSTAEVRDKQQNILASWHYTRKDPGDTFSNFISHWLFPA
                     PIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAVLTGCILLASGIAITLTRHLHNGL
                     VEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRFALDFNSLLDEMEEWQLRLQAKNA
                     QLLRTALHDPLTGLANRAAFRSGINTLMNNSDARKTSALLFLDGDNFKYINDTWGHAT
                     GDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPF
                     DLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            12159..12641
                     /gene="yfiB"
                     /locus_tag="ACHOT6_001861"
     CDS             12159..12641
                     /gene="yfiB"
                     /locus_tag="ACHOT6_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFP9947091.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12718..13065)
                     /gene="rplS"
                     /locus_tag="ACHOT6_001862"
     CDS             complement(12718..13065)
                     /gene="rplS"
                     /locus_tag="ACHOT6_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFP9947092.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13107..13874)
                     /gene="trmD"
                     /locus_tag="ACHOT6_001863"
     CDS             complement(13107..13874)
                     /gene="trmD"
                     /locus_tag="ACHOT6_001863"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFP9947093.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13905..14453)
                     /gene="rimM"
                     /locus_tag="ACHOT6_001864"
     CDS             complement(13905..14453)
                     /gene="rimM"
                     /locus_tag="ACHOT6_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFP9947094.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14472..14720)
                     /gene="rpsP"
                     /locus_tag="ACHOT6_001865"
     CDS             complement(14472..14720)
                     /gene="rpsP"
                     /locus_tag="ACHOT6_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFP9947095.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(14969..16330)
                     /gene="ffh"
                     /locus_tag="ACHOT6_001866"
     CDS             complement(14969..16330)
                     /gene="ffh"
                     /locus_tag="ACHOT6_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFP9947096.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16497..17288
                     /gene="ypjD"
                     /locus_tag="ACHOT6_001867"
     CDS             16497..17288
                     /gene="ypjD"
                     /locus_tag="ACHOT6_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFP9947097.1"
                     /translation="MPVFALLALVTYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17309..18595
                     /gene="yfjD"
                     /locus_tag="ACHOT6_001868"
     CDS             17309..18595
                     /gene="yfjD"
                     /locus_tag="ACHOT6_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFP9947098.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGSANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            18717..19322
                     /locus_tag="ACHOT6_001869"
     CDS             18717..19322
                     /locus_tag="ACHOT6_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFP9947099.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(19357..19947)
                     /gene="grpE"
                     /locus_tag="ACHOT6_001870"
     CDS             complement(19357..19947)
                     /gene="grpE"
                     /locus_tag="ACHOT6_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFP9947100.1"
                     /translation="MSSKEQKTPEGQATEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            20069..20947
                     /gene="nadK"
                     /locus_tag="ACHOT6_001871"
     CDS             20069..20947
                     /gene="nadK"
                     /locus_tag="ACHOT6_001871"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFP9947101.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            21033..22694
                     /gene="recN"
                     /locus_tag="ACHOT6_001872"
     CDS             21033..22694
                     /gene="recN"
                     /locus_tag="ACHOT6_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFP9947102.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGATRADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYTNETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     MATPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22843..23184
                     /gene="bamE"
                     /locus_tag="ACHOT6_001873"
     CDS             22843..23184
                     /gene="bamE"
                     /locus_tag="ACHOT6_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFP9947103.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(23246..23536)
                     /gene="yfjF"
                     /locus_tag="ACHOT6_001874"
     CDS             complement(23246..23536)
                     /gene="yfjF"
                     /locus_tag="ACHOT6_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFP9947104.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(23526..23963)
                     /gene="ratA"
                     /locus_tag="ACHOT6_001875"
     CDS             complement(23526..23963)
                     /gene="ratA"
                     /locus_tag="ACHOT6_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFP9947105.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            24134..24616
                     /gene="smpB"
                     /locus_tag="ACHOT6_001876"
     CDS             24134..24616
                     /gene="smpB"
                     /locus_tag="ACHOT6_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFP9947106.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24831..25193
                     /gene="ssrA"
                     /locus_tag="ACHOT6_001877"
     tmRNA           24831..25193
                     /gene="ssrA"
                     /locus_tag="ACHOT6_001877"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(25354..28609)
                     /locus_tag="ACHOT6_001878"
                     /pseudo
     CDS             complement(25354..28609)
                     /locus_tag="ACHOT6_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(28674..28793)
                     /locus_tag="ACHOT6_001879"
     CDS             complement(28674..28793)
                     /locus_tag="ACHOT6_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9947107.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(29017..29169)
                     /locus_tag="ACHOT6_001880"
                     /pseudo
     CDS             complement(29017..29169)
                     /locus_tag="ACHOT6_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(29522..>29795)
                     /locus_tag="ACHOT6_001881"
     CDS             complement(29522..>29795)
                     /locus_tag="ACHOT6_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947108.1"
                     /translation="LFDLDTPCIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKL
                     ITGVAPFDDILFMDDDFDDSSSEDDPVENSPVVTSPVVSSSKSSFQ"
BASE COUNT         7557 a         7820 c         6864 g         7554 t
ORIGIN      
        1 aattctcagg agaaccccgc tgacccggcg gcgtgtttgc cgttgttccg tgtcagtggt
       61 ggcgcattat agggagttat tccggcctga caagcgaaaa atataaaaac tttatcgttc
      121 gctcattttt caggcaaaac atcttaaata tagtcttttc cgtctaactt atagacaaaa
      181 acgagccccg aagggctcgt tttatcattt actggacggc gacaatccgg tcttcattaa
      241 cttccaggcg aatcacttta cccggaacca attcaccaga cagtatttgc tgtgccagcg
      301 ggttttcgat ctgctgctgg atagcacgtt tcaaaggacg tgcaccatag actggatcgt
      361 aaccgttctc gctcagcagt ttcagcgcct cgtcagaaat gtggatttca taaccacgtt
      421 cttccagacg tttgtacaga cgtttcaact gaatctgcgc aatcgaggca atgtgctgtt
      481 cacccagcgg atggaagacc accacttcat caatacggtt aatgaattcc ggacggaagt
      541 tatggcttac cacaccgagc accagctctt tcatgtgcgc ataatccagt tcaccgaagc
      601 gttcctgaat caaatcggaa ccgaggttag aggtcataat gacaaccgta ttacggaagt
      661 cgaccgttct cccctgcccg tcagtcagac gaccatcatc cagtacctgc aacagaatgt
      721 tgaagacatc cggatgcgct ttttccactt catccagcag gatgacggaa tacggacgac
      781 gacgcacagc ttcggtcagg tagccacctt cttcataacc gacatatccc ggaggcgcac
      841 caaccagacg agacaccgag tgtttctcca taaactcgga catatcgata cggaccatcg
      901 cctcgtcgct atcaaacata aagttcgcca gcgccttaca aagctcagtt ttccccacac
      961 ccgttgggcc gaggaacagg aatgaaccaa tcgggcgatt tggatccgcc aaccccgcac
     1021 ggctacggcg aatagcgtta gataccgcat caaccgcttc gttctgacca atgacgcgat
     1081 ggtgcagttc ttgctccata cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa
     1141 ccggaatccc cgtccaacgc gccagcactt cagcaatttc ggcgtcggtc actttattac
     1201 gcaacagacg catagttttg ccttcgagct gcgttgcggc ttccagttgc ttttccagtt
     1261 ccgggatttt gccgtattgc agttcagaca tccgcgccag gtcccccaca cggcgagcct
     1321 gttcaatagc gattttcgcc tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg
     1381 atgccttctc tgctttccac tcttcttcta actcggagta ctgacgttct ttgtcgctca
     1441 gttcttcgtt gagcatatcc aggcgttttt tactggcttc atcagactct ttcattaacg
     1501 cctgttgttc cagtttgagc tggatgatac gacgatcgag tcggtcgagt tcttctggtt
     1561 ttgagtcaat ctgcatacga atgctggatg ctgcttcatc gatcaggtcg atggctttat
     1621 ccggcagctg acggtcagca atgtagcgat gagacaacgt cgccgctgca acaattgccg
     1681 ggtcagtaat ttgcacatgg tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg
     1741 caatggtatc ttcaacagaa ggctcggcaa caaacacttt ctggaaacga cgttccagcg
     1801 cagcatcttt ttcgatgtac tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca
     1861 attcaccacg cgccagcgcc ggtttcagca tgtttccggc gtccattgcg ccatcggctt
     1921 tacccgcgcc gaccatggta tgtaattcgt cgataaacag gatgacgttg ccttcctgtt
     1981 tggcaagatc gttaagcacg ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc
     2041 cagccaccag cgcgcccata tccagcgcca gtacgcggcg gcctttcaat ccttccggca
     2101 cttcgccgtt gataatacgc tgcgccagac cttcaacgat ggcagtttta ccgacgccgg
     2161 gttcaccaat cagtaccggg ttatttttag tacgacgttg cagcacctga atggtacggc
     2221 gaatttcttc atcacgacca atcaccggat caagtttgcc ctgttcggct cgttcggtaa
     2281 ggtcgatggt atattttttc aaagcctgac gttggtcttc agcaccttga tcgttcacgc
     2341 tttcacctcc acgcatttgt tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg
     2401 ctttcaggat gtcggccagc gtgccgcgag actcaagtgc cgccagaacg aacagttctg
     2461 acgagataaa gttatcacca cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca
     2521 ccagatcctg tgatggctgg acatcaccac cagtaccttc aacctgcggt aaacgattta
     2581 atgcctgatt gatatctgtg cgcaactggc cagcatttat gccagcggat gttaataaag
     2641 gactaaccga acccccttcc tgattcagca gggcgctcat taaatgaagt ggttcgataa
     2701 attggttgtc gtgcccgagt gcaagtgatt gggcatcggc aagagcaagc tggaatttat
     2761 tagtaagacg atccagacgc ataactcctc ccataacgga tcaaagttgc tactggagat
     2821 taaatgaggt catccctcaa ttattcaagg ttattgacca gattaatgtg aaaagaaaat
     2881 cacgcgtacc ggatcgtctt gattctttag gttatatcag ccaaatgaaa cttgccatac
     2941 gaccggtggt cttgtcgcga cgataagaga agaaagtctc attttccgta tatgtacaac
     3001 ggtcgccgcc gaaaatttgc tcaacaccca cgttcgccag acgctgccgg gcaagctgat
     3061 aaatatccgc cagatactta tcaccatgct gaatgaaagc tgcacttgct ttagcgtcta
     3121 ctgccataaa cgcctcgcga acctccaccc ccacttcgaa cgcgcgtgga ccaattgccg
     3181 gccctaacca ggcgagaata ttttccggat tatcagcaaa acaggaaacc gtctcttcca
     3241 gcacgcctgc gcacagtcca cgccagccag catgagcggc ggcgacttcc gttcccgctc
     3301 gattgcaaaa cagcacaggg aggcagtcgg cagtcatcac tgcgcaaacc gtgccgggcg
     3361 tattgctata agaggcatct gctcgttttg aggcataagg ttcgccagta agcttaagca
     3421 catctttgcc gtgtacctgc tcaagccaga ccggtttaga aggcaaattg cccgcagcaa
     3481 aaagtcgctt gcgattctcc tcaacgtgat ccgggttatc gccacaatgg gcaccgaggt
     3541 tgagcgagtc atacgggggc aagctcacgc cgccgatacg agaagagcta caggccgcaa
     3601 caccttttgg tagcggccac tgcgggacaa tcagcttact cataaccagt ccacttcatc
     3661 cttatgctct tcgaaatcgg cgcgcatcac ctcaatcagc tccaccatat cttgtggaat
     3721 aggcgtatgc cattccattt cgatgccgga gatcgggtga taaagacgca gcatggttgc
     3781 atgaagcgcc tggcggtcaa acttacgcag catggagata aatgcttccg aagcaccttt
     3841 tggcggacgc ggacggccac cataaaccgg atcgcccacc agcggatgag tgatatgggc
     3901 catatgcacg cggatctggt gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg
     3961 cacacggaag tgttccatga tgcgatagtg agtcaccgcc ggtttgccca tcggatgcac
     4021 cgccatgtga gtacgtttgg tcgggtggcg gctgatgggt tcatctactg taccgccagc
     4081 ggtcatatga ccaatcgcca ccgcttcata ctcacgagta atttcacgcc gttgcaaaga
     4141 ttcgactaaa cgcgtctgag ccggaacggt ttttgccaca accatcaggc cagtggtgtc
     4201 tttatccaga cgatggacga tgcccgcacg tggtacatcg gcaatgggcg gatagtaatg
     4261 aagcaacgca ttcagtaccg tgccatccgg gttacccgcg ccaggatgta ccaccaggtc
     4321 gcgcggttta ttaatgacga tgatatcttc atcttcatag acgatatcca gcgggatatc
     4381 ctgcggttca aaacgcgctt cttcttcaat ctcagcgttg atggcaacct gctcgccacc
     4441 caatactttt tctttcggct tatcacaaac tttgccgttt accagcacgc gctggtcgag
     4501 gatccattct tttattcgcg aacgtgaata atccgggaac atttcggcca aagcctgatc
     4561 taagcgttga ccgagttggt tttcggacac cgttgcagtg agctgtactc gttgtgccat
     4621 atatactgct tcttcgttta tcgttggggt tttacggctt tgccgtttaa tatagtgtgc
     4681 tattgtagct ggtcttaacc gggagcagga acagagaatc tcccgtatta cattttgagg
     4741 aaagtcaaaa cgtcatgacg cgcatgaaat atctggtggc agccgccaca ctaagcctgt
     4801 ttttggcggg ttgctcgggg tcaaaggaag aagtacctga taatccgcca aatgaaattt
     4861 acgcgactgc acaacaaaag ctgcaggacg gtaactggag acaggcaata acgcaactgg
     4921 aagcgttaga taatcgctat ccgtttggtc cgtattcgca gcaggtgcag ctggatctca
     4981 tctacgccta ctataaaaac gccgatttgc cgttagcgca ggctgccatc gatcgtttta
     5041 ttcgccttaa cccgacccat ccgaatatcg attatgtcat gtacatgcgt ggcctgacca
     5101 atatggcgct ggatgacagt gcgctgcaag ggttctttgg cgttgaccgt agcgatcgcg
     5161 atcctcaaca tgcacgagct gcgtttagtg acttttccaa actggtgcgc ggctatccaa
     5221 acagtcagta caccaccgat gccaccaaac gtctggtatt cctgaaagat cgtctggcga
     5281 aatatgaata ctccgtggcc gagtactata cagaacgtgg cgcatgggtt gccgtcgtta
     5341 accgcgtaga aggaatgttg cgcgactacc cggataccca ggctacgcgt gatgcgctgc
     5401 cgctgatgga aaatgcatac cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga
     5461 aaatcatcgc cgcaaacagc agcaatacat aacagaaacc tgaaatacaa aacggcagcc
     5521 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
     5581 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
     5641 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat tcccatcaca
     5701 cattttgaca tcaggagcgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
     5761 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
     5821 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
     5881 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
     5941 ttctggttgc cagtggtaaa catgaagata tgtacactgc aattaacgaa ttgatcaaca
     6001 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
     6061 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
     6121 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
     6181 gtactaaagt cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt
     6241 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
     6301 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
     6361 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
     6421 cattactggc agagcggcgc gaattggccg tcgaggtagg aaaagccaaa ctgctctcgc
     6481 atcgcccggt acgagatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg
     6541 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     6601 attccgtatt aactcagcag gctttgctcc agcaacatct caataaaatt aatccgcact
     6661 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     6721 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     6781 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     6841 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     6901 agatgacgtt aactatcgac cattgtttgt tggtctccgg cacgactgat ttatccacca
     6961 ttaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     7021 atcctcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     7081 aggcaaaatc accgcatgtt gctgcgttgg gaagcgaagc tggcggcact ttgtacggtt
     7141 tgcaggtact ggagcgtatt gaagcgaatc agcaacaaaa cttcacccga tttgtggtgt
     7201 tggcacgtaa agccattaac gtgtctgacc aggttccggc gaaaacgacg ttgttaatgg
     7261 cgaccgggca acaagccggt gcgctggttg aagcgttgct ggtgctgcgc aaccacaatc
     7321 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt
     7381 atctggatat tcaggccaat cttgaatcag cggaaatgca aaaagcattg aaagagttag
     7441 gggaaattac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     7501 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     7561 tggcggttgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     7621 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     7681 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     7741 ttacgctctg acgacataat aatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     7801 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     7861 tgaacatttt cttctgccag atgcagccca taagcaaaag tagcaaagtg gcgcagagcc
     7921 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcag ccgagcgccc
     7981 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     8041 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     8101 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     8161 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     8221 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     8281 cagtcatgtt gctccagaat ccgcacctga taacccgata gtgtcagcat cttctcgaac
     8341 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     8401 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     8461 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     8521 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     8581 acttcagcaa ccagttccag acgcttcgct aataaattca gcagcgcttt atcgacttca
     8641 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     8701 agccgtcagc tgcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     8761 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     8821 agaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     8881 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     8941 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     9001 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     9061 gatgacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     9121 aatgccaaca aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     9181 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     9241 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     9301 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     9361 ctcaagcagc aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     9421 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     9481 gcgcattacc agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc
     9541 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     9601 aggatcgcgc ccggcgataa tatctgaaat ggttctacgc gagtcagcaa tctgggcttc
     9661 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     9721 gtcggtaata tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct
     9781 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     9841 tcaatccata tttcgtacat ttttatttac acaggcaatt tagtcgcgat ttcaaccctt
     9901 acttctgtat agataaattt acactccctt tgaaaacaat ccgctatgct ttgaaaaagg
     9961 agaaagaaat gatgaaaaag tttatcgccc ccttgttggc tttactggtt agcggatgtc
    10021 agattgatcc ttatactcac gcgccaactt tgaccagcac cgactggtat gatgttggta
    10081 tggaagatgc gatatcgggc agcgccataa aagatgacga tgcatttagc gattcacagg
    10141 cggatcgcgg tctatacctt aaaggatatg ccgaaggaca aaagaaaact tgccagaccg
    10201 attttactta tgcccgagga ctttccggta aaagctttcc tgccagctgc aacaatgttg
    10261 aaaatgccag ccaactgcat gaagtatggc aaaaagcggc tgatgaaaat gccagcacaa
    10321 tacgattaaa ttaattaaaa ccccacaaat aaattaaaca taagatttta ctcattgtgt
    10381 tttatttctc acattgatga cgatcgcatg aaaaaatgat attttaatga gcattctgaa
    10441 aaaatatcga tgcatttcga gcgaagatgg tggggatccc tgaatgcgtt tttctcaccg
    10501 actgttctta cttctgattc tcctgctgac gggagcacca atactcgctc aggagccttc
    10561 tgacgttgcg aaaaacgtgc gtatgatggt ttccggtatt gtcagttaca cccgctggcc
    10621 tgcgctatca gggccaccaa agttatgtat cttttcatct tcacgtttta gtaccgccct
    10681 acaagagaat gccgcgacat ctttgcctta tctgcctgtc atcattcaca ctcaacaaga
    10741 ggcgatgatt tcaggctgca atggttttta ttttggcaac gagtcaccga cattccagat
    10801 ggaattaacc gaacaatatc cgtcaaaggc gttattatta attgccgaac aaaataccga
    10861 gtgcattatt ggtagcgcct tttgtctgat catccacaat aatgacgtca gatttgccgt
    10921 aaacctggat gccttatcgc gtagcggtgt aaaggtcaac ccggatgtct taatgctcgc
    10981 acggaagaaa aatgatggat aacgataatt ctcttaataa gcgccccacg tttaaaagag
    11041 cattacgcaa catcagtatg accagcatat ttatcactat gatgctgatc tggttgctgc
    11101 tttccgtgac ctcggtgctg accctgaaac agtacgcgca aaaaaacctg gcactggcag
    11161 cgctgggcca gcaagggcaa ttttcaactg cagaagtacg tgataagcag caaaatattc
    11221 tggcatcctg gcattacacc cgtaaggatc caggcgatac tttcagcaat ttcataagcc
    11281 actggctctt ccctgccccc atcattcagc cgattcgtca caatggcgaa accattggcg
    11341 aagtacgctt aaccgctcgc gacagttcaa tcagccattt tatctggttt tcgctcgccg
    11401 tactgaccgg ttgtattctg ctggcatcag gaatcgcaat taccctcacc cgccatttgc
    11461 acaatggcct ggtggaagca ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca
    11521 accgcaattt ttcccgacga gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc
    11581 tcgacttcaa cagtctgttg gatgaaatgg aagagtggca gcttcgttta caggctaaaa
    11641 atgcgcagct tctacgtacc gcgctacatg acccattaac cgggctggct aaccgcgcag
    11701 cgtttcgtag cggcatcaac acgttgatga acaattccga tgcccgaaaa acgtcggcgt
    11761 tactatttct tgatggcgat aatttcaaat acatcaatga tacctggggt catgcgacgg
    11821 gcgatagagt cttgattgaa atcgcaaaac ggttagctga agttggcggg ctgcgacata
    11881 aagcataccg cctgggcggc gatgaattcg ctatggtgct ctatgatgta cagtcagaat
    11941 ctgaagtgca gcagatatgc tcagcactga cacaaatctt taatctcccg tttgatcttc
    12001 ataatggcca tcagaccacc atgacattaa gcattggtta cgcgatgacc attgagcacg
    12061 cctctgcgga aaaattacaa gagcttgccg atcacaatat gtatcaggcc aaacaccagc
    12121 gtgccgaaaa gctggtgaga taacaaggat atatcgatat gataaagcac ctggtagcac
    12181 ccctgatttt cacctcacta atactgactg gctgccagtc ccctcaggga aagtttactc
    12241 ctgagcaagt cgccgctatg caatcttatg gatttactga atccgccggc gactggtcgc
    12301 tgggcttatc agatgccatt ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc
    12361 agcaacagat ccaaaccatg gcagccaaat tggcctcgac agggctaaca catgcccgta
    12421 tggatggaca caccgataac tatggtgaag acagttacaa cgaaggctta tcattgaaac
    12481 gggcgaatgt cgtggccgat gcatgggcta tgggtggaca aattccacgc agcaatctca
    12541 ccacacaggg tttaggaaaa aaatatccca tagccagtaa caagaccgcc cagggccgcg
    12601 ccgagaaccg ccgcgtcgca gtggtgatta caacccctta aaacacgagc aaacagacaa
    12661 aaaaagggcc agccaattgg ccagcccttc ttaacaggat gtcgcttaag cgaaatctta
    12721 gttaagacgc tctttgatac gagcagcctt accagtacgc tcacgcaggt agtacagttt
    12781 agctttacga acagcaccac gacgtttgac agaaatgctg tcaactaccg gagagtgagt
    12841 ctggaagaca cgctcaacgc cttcgccgtt ggaaatttta cgaacagtga atgcagagtg
    12901 cagaccgcgg ttacgaatag cgataaccac gccctcgaat gcctgcagac gttttttgga
    12961 accttcaaca acccatactt tcacttccac ggtatcaccc ggacggaagg aaggtacgtc
    13021 ctgcttcatc tgctcttgtt caagttgctt aataatgttg ctcataattt aatctcttat
    13081 cctgggtaaa ctgatatctc gggggcttac gccatcccat catgtttatg ttgctgttgt
    13141 gcgtgttccg ttttgaactc cgccagcaac cttgcttgct cttcagtcag agccaggttt
    13201 tccagaagtt caggtcttct aagccaggta cggcccagcg actgtttcaa acgccagcga
    13261 cgtatctcgg catggttgcc cgacagtaac actggcggaa cttccatccc ttctaacacc
    13321 tcaggccgcg tatagtgcgg gcaatccagc aatccttcag caaaggaatc ttccgttgcc
    13381 gaggcttcat gtcccagtac tcccggaata aaccgggaaa cggagtcaat cagcgtcatt
    13441 gctggtaact caccaccact gagaacgtaa tcgccgattg accattcttc gtcaatttcg
    13501 gtttggatca cgcgctcatc tataccttcg tagcgaccgc acaccagaat caatttttga
    13561 ttcgttgcca gttcgctgac gcccgcttga tcaagcttgc gtccctgtgg tgacagataa
    13621 atcacctttg cgccttcacc cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt
    13681 tgcaccatca ttaacatccc cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc
    13741 cggtcatgcg tgaagtcgcg aggactccag ctctggatgc tcagcaggcc atttttaact
    13801 gcccggccag ttaccccgta atcggtaatt gcgcggaaca tttcaggaaa caggctaatt
    13861 atgccaatcc acatagcgcc gtcttttacc gtttatccgg tggtttaaaa accaggatcc
    13921 caatctactt cgattgaacg agtagtgaga tcgactttct tgataacctg cccatcgagg
    13981 aacggtacga gacgttcctt gataccaaac gcatctttca ggtttgcctt aatgacgaga
    14041 acgtcattag atccggtttc catcatatcg acgactttac cgagatcgta gccttcagtg
    14101 gttactacct ggcagcccat caggtctttc cagtagtagt ccccctcttc aagctgaggc
    14161 agctgcgatg aatccacgac aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg
    14221 tcaacgcctt tcagcttgat gatcatgtcc tgattgtggt gcttccagct ttccagctgg
    14281 acttgctgcc actgacccgc cttctggata aaccaaggct gatagtcaaa aatgctttcg
    14341 gcgtcttcgg tggaagaaaa cactctgagc cacccacgaa taccgtaaga cgaacccatt
    14401 tttcccaaaa cgatgggatc aacaggtgct tgcgcggtga gttgtttgct catcatgacc
    14461 accgtgacag attaagctgc tttgtttact tctttgatca gcgcagcaac gcgatcagaa
    14521 atagttgcgc cctggccaac ccagtgagcg atgcgatcca gatccaggcg agtgccttct
    14581 tctttttcgc tagcgattgg gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt
    14641 gcattacggc tgtcagcgac aacaacctgg tagaacggac gctttttagc gccgtgacgt
    14701 gctaaacgaa tagttaccat aacatcctct tgtgtgaata aaacaaccgg accccatcga
    14761 ggaacggagt ccggtgtcat attaaaagcc cgaaaatttt actcattttt gcgggaattg
    14821 caatcaacag ttgctaactc tgctgtaaaa ggccgtcggc ggtgcagcca gtttggtgcc
    14881 ggagtgcgcg cagtcaccgg agcgtacacg cagtacgtga ggatgacgag cacatcccgg
    14941 tgccaaaatg gcaaacaagc caggccgatt agcgaccagg gaagcctggg ggcatcatac
    15001 ccttcatgct tctcatcatc ttcgccattc cgcctttctt cattttcttc atcatgcgct
    15061 gcatgtcgtc gaactgcttc agaaggcggt tcacgtcctg cacctgcata ccgcaaccgg
    15121 cagcgatacg acgcttacgc gaacctttga tgatttctgg cttagcgcgc tctttcatcg
    15181 tcatcgagtt gatgatggct tccatacgca ccagcacttt atcgtccatc tgcgacttaa
    15241 cattatccgg gatctgcccc atgcccggca gcttgcccat caggctagcc atgccgccca
    15301 tatttttcat ctggcgcagt tgctcaagaa aatcgttgag atcgaagccg tcgccttttt
    15361 tcagcttgct ggctaatttc tctgcctgcg cgcggtcaac tttgctttcg atatcttcga
    15421 tcagcgacag tacgtcgccc atgccgagaa tacgcgacgc gatgcggtcc ggatggaacg
    15481 gctccagcgc ctcagttttc tcgccaacac cgagaaactt gatcggtttg ccagtgatgt
    15541 gacgaataga gagcgccgca ccgccgcggg catcgccgtc cactttggtc aacactacgc
    15601 cggtaagcgg taacgcttca ttgaatgctt ttgccgtatt ggccgcatcc tgaccggtca
    15661 tggcgtcaac cacaaacagg gtttcaaccg ggttaatcga cgcatggact tgtttgatct
    15721 cgtccatcat cgcttcgtca acgtgcagac gaccagcggt atccaccagc agcacgtcgt
    15781 agaatttcag tttggcttct ttcagcgccg cgttaacgat atctaccggc ttctgaccaa
    15841 catcagaagg gaagaaatca acgcccacct gctctgccag cgtctcaagc tgtttgattg
    15901 ccgccgggcg ataaacgtcg gcagaaacca ccagcacttt cttcttgtgc ttctcgcgca
    15961 ggaacttacc gagtttacca acgctggttg ttttaccggc accttgcagg cccgccatca
    16021 gcacgaccgc aggcggttgc gccgccaggt tcagggtctg gttctcttcg cccatcgccg
    16081 caaccagttc gttacggacg attttgacga actcctgccc cggcgtcagg ctcttattaa
    16141 cttcatgacc aaccgctttc tcttttacgc gattgataaa ctcacgcact accggcagag
    16201 cgacgtccgc ctccagcagc gccatgcgca cttcgcgcag cgtatctttt acgttgtctt
    16261 cagtgaggcg tccacggcca ctgatattgc gcagcgtgcg cgacaaacga tcggttaaat
    16321 tatcaaacat tgtctctcgc ctggggtgga aacggttggc cgcaatcgcg acacatcatc
    16381 agtattttgc cgcagtataa catgaaggcg tctttgttgt tatgcaacgg ttggagcagc
    16441 gttcacctga cgctatactg cttctctttc ttattgctca aactgtcgac atcactatgc
    16501 ccgtttttgc tctgctcgcg cttgtcacct actccgtcag tcttgcgctg attgttcccg
    16561 gtctgctgca aaaaaacggc ggctggcggc gcatggctat tatttctgcg gtcattgcgc
    16621 tggtctgcca cgcaatcgct ctggaagccc gcatcctgcc cgacggtgat agcggacaaa
    16681 acctcagcct gctgaacgtt ggttcattgg tcagtttgat gatctgtacg gtaatgacca
    16741 ttgtggcttc tcgcaatcgt ggctggttgc tgctacccat tgtctatgcc tttgcgctta
    16801 tcaacctggc gctggcgacc ttcatgccca atgaatacat cacccatctg gaagcaacgc
    16861 ctgggatgct ggtgcacatt ggcttatcgc tcttttccta tgccacgcta attatcgccg
    16921 ccctgtacgc gctgcaactg gcgtggattg attaccaact gaagaacaag aagctggcat
    16981 ttaaccagga aatgccgcca ttgatgagta tcgagcgtaa aatgttccac atcacgcaga
    17041 ttggcgtggt gctgctaacg ctcacgcttt gcactggcct gttctacatg cacaacctat
    17101 ttagcatgga aaatatcgac aaggctgtgc tctctatcgt ggcgtggttt gtctatattg
    17161 tgctgctgtg gggacattac catgaaggat ggcgtggacg ccgcgtcgtc tggtttaacg
    17221 ttgcgggcgc ggtaattctg acactggcct acttcggcag ccgaattgtc cagcagttaa
    17281 tcagctaaac ccagaaaagg agtttcccct ggaacacatt tctactacta cgttgatcat
    17341 tattctgatc atcatggtgg tcatttcagc ctatttttcc gggtccgaaa ccggaatgat
    17401 gaccctcaac cgctatcgtc tgcgacatat ggcgaaacag ggtaatcgct cggccaaacg
    17461 cgtcgaaaaa ttgctgcgta agccagaccg cctgataagc ctggtgttaa tcggcaataa
    17521 cctggtcaat attcttgcct ccgcgctcgg cactattgtt gggatgcgtt tgtacggcga
    17581 tgcgggcgtg gcaattgcga ctggtgtgct gacttttgtc gtactggtat ttgctgaggt
    17641 attgccgaaa accattgccg cgctgtaccc ggaaaaagtc gcttatccga gtagttttct
    17701 gctggctccg ctgcaaattt tgatgatgcc gctggtctgg ttgctgaatg ctatcacccg
    17761 tatgctgatg cgcatgatgg gtatcaaaac cgatatcgtg gttagcggct ctttgagcaa
    17821 agaagagttg cgcactatcg tgcacgaatc acgctcacaa atttcccgtc gcaatcagga
    17881 tatgctgctg tcggtgctcg atctggaaaa aatgaccgtt gatgacatca tggtgccgcg
    17941 cagtgaaatt atcggtattg atatcaacga tgactggaaa tcgattctgc gccaactctc
    18001 ccactcacct cacgggcgca tcgtgctcta ccgtgattcg ctggacgacg ccatcagtat
    18061 gctgcgagta cgtgaagcct ggcggttaat gtcggagaaa aaagagttca ccaaagaaac
    18121 catgctgcgc gccgcggacg agatctattt tgtgcccgaa ggtacgccgc tcagcacgca
    18181 gttggtaaag tttcagcgca acaaaaagaa agtcggcctg gtcgtcaacg agtatggaga
    18241 cattcagggg ctggtgacgg ttgaagatat tctggaagag attgtcggcg atttcaccac
    18301 gtcgatgtcg ccaacacttg ccgaagaggt cacgccgcaa aacgacggtt cggtgattat
    18361 tgatggctct gccaacgtgc gagaaatcaa caaagccttt aactggcatc taccggaaga
    18421 tgatgctcgt actgttaacg gtgtcattct ggaagcgctg gaggagatcc cggtagcagg
    18481 cacccgcgtg cgtattggcg agtacgatat cgatattctc gacgtacagg acaatatgat
    18541 taagcaggta aaagtttttc ctgtgaaacc gctgcgcgag agtgtggcgg agtaacgcaa
    18601 tggcgaactc cggttcgctt ttttttatcg ccgtctttta ggattccatc aattcctgtc
    18661 atttacttag caatgtaata atgtcttttc aagcattcgg aaaggatatt gatgttatgt
    18721 cagaagatta tgtaattgaa tgggataagg attttgcaga cgatctcaac gtcgtcgcca
    18781 gcgttttttt acagcacacc ccaaccttat ggcccactat tttctctcaa ctttcgacgc
    18841 aacctgaaat ctttgaagac gaagatgaag acgaatatgg attgcaggac gttctcgatt
    18901 gcagtggtgg cgatctcggg aatcgtgacc tggcgcaggc atttttacag gttctacgcg
    18961 gtgaaggatt aattcaactc gttgactgga aaggtgaaga tgaagaaggt gagctggcta
    19021 attttgctgc agatcgcttt tatgagctca caaaaaacct gatagcttct gaggaattaa
    19081 ggagtcttct tgtcgaaata acccaagagg atgaaatctc cgatgtctgt gaagccggag
    19141 accgctacct tgacgaaatc tttgaacgca tccaaaccga actcaataaa aggggctttc
    19201 agatttttga tctgaatgaa ggatctgaca cctataacgt ggtcgttctg ccaatgaacg
    19261 aatataaaaa aatagatgat ttcaacacgc cgtggctgga agtacaggat tttttaagct
    19321 aaaaaaatgg ccgacttcgc gtcggccatc agatatttac ttcgccttcg ctacagtaac
    19381 catcgccgca cgaatcgtac gaccattcag cgtataaccc ttctgcataa tgccgagtac
    19441 gttacctggc gcaacgtcat cagattccac cattgcgatg gcctgatgca cattcgggtc
    19501 cagtgggacg ttagtttcgg cgatcacttc aacgccaaac ttacgcacaa catccagcat
    19561 cgacttcagc gtcagctcaa tgccttcaac catcgcagac atatccgggt tagctttatc
    19621 agccacttcc agcgcacgat ccaagctatc aatcaccggc agcaattcgt tgatgaattt
    19681 ctccagcgcg aatttgtggg ctttttcaat atccagttca gtacgacgac gcaggttttc
    19741 catttcggct tttacacgca aaatgccgtc acgttcacgg gtctgggctt cagccagctg
    19801 agcttcgaga ttcgcaattt tttcatcgcg cggatccacc tgctcagcag aagcttctgg
    19861 ctcaactgcc tcaatctctt cgtgctgatc catgataatt tcttccgtgg cttgcccctc
    19921 aggcgttttc tgttctttac tactcatgaa tttctccgcg ttttttcgca ttcatctcgc
    19981 taacttcgct tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat
    20041 caatcttcgc aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt
    20101 gggacaccca cggcacccta ctgcactgac aacacatgaa atgctctacc gctggctgtg
    20161 cacaaaaggt tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa
    20221 tgtgaaaact ggcacgctcg cggagattgg gcaactggct gatctcgcgg ttgtcgttgg
    20281 cggcgacggt aatatgctgg gcgcggcgcg cacgctcgcc cgctacgata ttaaagttat
    20341 tggaatcaac cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca
    20401 acaattagcc gatgtgctgg aaggtcacta catcagcgag aaacgttttt tgctggaagc
    20461 gcaagtctgt cagcaagatt gccagaaacg catcagcacc gcgataaatg aagtggtact
    20521 tcatccaggc aaagtggcgc atatgattga gttcgaagtg tatatcgacg agatctttgc
    20581 gttttctcag cgttccgatg gactaattat ttcgacgcct acaggctcca ccgcctattc
    20641 cctctctgca ggcggtccta ttctgacacc ctctctggat gcgattaccc tggtgcccat
    20701 gttcccgcat acgttgtcag cacgaccact ggtgataaac agcagcagca cgatccgtct
    20761 gcgtttttcg catcgccgta acgacctgga aatcagttgc gacagccaga tagcactacc
    20821 tattcaggaa ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca
    20881 tccgaaagat tacagttatt tcaatacatt aagcaccaag ctcggctggt caaaaaaatt
    20941 attctaattt tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata
    21001 acagtaatgg tttttcatac aggaaaacga ctatgttggc acaactgacc atcagcaact
    21061 ttgctatcgt tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg
    21121 agaccggcgc gggtaaatct attgcaatag atgccctcgg tctttgtctc ggtggtcgcg
    21181 ctgaagccga catggtgcgt accggcgcta ctcgcgctga cctgtgcgcc cgtttttctc
    21241 tgaaagatac gccagcggcc ctgcgctggc tggaagaaaa ccagcttgaa gacgggcatg
    21301 aatgtttgct tcgtcgcgtg atcagcagtg atggtcgctc ccgtggtttc atcaacggta
    21361 cagctgttcc tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag atccatggtc
    21421 agcacgctca tcagttactc accaaacccg agcaccaaaa attcctgctt gatggctata
    21481 ccaatgaaac ctctctgctc caggaaatga ccgcacgtta tcagttgtgg catcaaagct
    21541 gccgtgacct cgcgcatcat caacagttaa gtcaggaacg cgctgcccgt gcggaactgt
    21601 tgcaatacca attaaaagaa cttaacgaat ttaatccgca gcccggagag tttgagcaaa
    21661 tcgacgaaga gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg
    21721 cattggcatt aatggccgac ggtgaagacg caaacctgca aagtcagctt tacacggcta
    21781 aacaactggt gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc
    21841 tggaagaagc taccatccag attgctgaag ccagcgatga actgcgccac tactgcgatc
    21901 gtctggatct cgatcccaac cgactgtttg aacttgaaca gcgcatctca aaacagattt
    21961 cgctggcacg taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac
    22021 tggaagaaca gcagcaacta gacgatcagg ccgactcaca agaaacgctc gcgctggcgg
    22081 taacgaaaca tcatcagcag gcactggaaa ccgcgcgcgc attacaccaa caacgccagc
    22141 aatatgcaga agaacttgca cagctgatca ccgacagtat gcatgcgctc tcaatgccgc
    22201 atgggcagtt tacgatcgat gttaaatttg acgagcatca cctaggcgct gacggtgccg
    22261 atcgtattga gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag
    22321 tcgcatccgg tggtgaattg tcccgcatcg cactggcaat ccaggtcatc acggcgcgta
    22381 aaatggcaac cccggcactg atttttgatg aagtggatgt agggattagc ggtccaacag
    22441 cggcagttgt cggcaaactg ctgcgtcaac tcggcgaatc aactcaggtg atgtgtgtta
    22501 cccacctgcc acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg
    22561 atggtgcgat gacagaaacg catatgcaat ccctgaataa aaaagcgcgg ttacaagagc
    22621 tggcgcgcct gcttggtggc agtgaagtca cacgtaatac actggcgaat gcgaaagaac
    22681 tgcttgcggc gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa
    22741 gaccggaaag caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga
    22801 tgagccacgt actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg
    22861 actgctgcag cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg
    22921 gtttaccgtc ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata
    22981 cgtgttggca tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat
    23041 ccatttggta cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta
    23101 acgcagcaaa cgctgacgct gacctttaac agtagcggtg tgttgaccaa tattgataac
    23161 aaacctgcgc tgagtggtaa ctaataataa agttgctctc aaagacgtta aaaaaggtgc
    23221 tcaatgagca ccttttttct gtccgttatt tattcgctga tttttctgct cgttgcctgc
    23281 gaagctcttt cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca
    23341 cactatcgct tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga
    23401 tatcggtacg caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct
    23461 cctgcagcgt cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg
    23521 caattttacc tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta
    23581 gccgccagct ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca
    23641 aagtcgagat gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca
    23701 ccaatcaatt tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc
    23761 aactggttgc gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca
    23821 gtcatctgcc caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga
    23881 ggataagact gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt
    23941 gcggtccggc taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc
    24001 atttatcctg ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa
    24061 gatagagcct tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg
    24121 attcacgacg cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct
    24181 taacaagcgc gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct
    24241 gcaaggctgg gaagttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt
    24301 ccttctgcgt gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc
    24361 ctccacgcat gtggtgtgcg atcctacccg tacccgcaag ttacttctca accagcgcga
    24421 actggactca ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct
    24481 gtactggaaa aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca
    24541 cgataaacgt tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa
    24601 aaacgcccac cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc
    24661 tccggcggcg cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc
    24721 attgcttaat cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat
    24781 tgaggctggt catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt
    24841 ctggattcga cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa
    24901 aaagccgcaa aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc
    24961 ttagagccct ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca
    25021 aaagagatcg cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt
    25081 tgttagtggc gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag
    25141 taccgaggat gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga
    25201 ttggacggta taaggacaac accaataaaa acaggaagtt agcagtctca gcaggacacc
    25261 gaccagacgg tgaagagaca taaaaggata cgcaaaggag ccgcggctcc tggtgacatg
    25321 aaagcccaca gatgtgggct ttttcgttga tggtcagaac gaccagttca caccagctac
    25381 cccgttccac ggggattcca caccggcacc atggctatac cccaccccaa gatgcccgct
    25441 taacgtactg ctgaatgagg ctttaatacc tgcctggtat attccacgtc tgcccgacaa
    25501 atcattgacg aaattaccgt cactattcac tttcacccgg ttatcatcga caaattcttt
    25561 gcgcacagcc gccttcagcc acggctcaac ttccataccg ttccccagac gcatgttgta
    25621 actcagcgtt gcacccagtt cacgatataa actgcgggta tcgactgatt tcgattccat
    25681 gccattggat aaatgatatt cagggttatc agcggtgaac ccccgttaac gatgcatacg
    25741 gcgtcaggtt ccagttacca tcggtaaatc gcatcccggt ttcaatgtga ccgcccagcc
    25801 cgttgctgcg ataactgccg ttggcggctc caccgctgcc aaccgtggag tcgcgagctt
    25861 cggtacctgc taataccaat gaattaccgc cattttccag caacatattg gtcgctaaat
    25921 tgccggaaat ggaaaaagtg ccgtactggt gagtaccgct gatttcaata ccgttagccg
    25981 tgctcgtctg gagagcggca ccgctgttct ggacgatatc tgtcgctttg ccattatcgt
    26041 taactgtcag cgtaccgcct tcattgatct ttgtttttat tgcctctccg ttagctgaaa
    26101 ctgtttgtat tccgccgtcg ttaatcgttg tctcattcgc caccccctcg acgatttgtt
    26161 cgccgccggt gagcgtcgtg cctgtcgcag tggcttttgt tttgacgatc tcccgtccgc
    26221 ccgtattgac ctgtgttttg tcagaagagg tgtctgactc cacggttaac acgccgccat
    26281 ttgccagcag gatattgttc gccgcaccct gctcgatgct gaacgcaacg ccatccgcgc
    26341 gtgttcctgt gacccgcgtc gccctggtgg ttgcaaccaa agcgccctgg ctactctgct
    26401 gtatccccgt tgcgctgcct ttctcccgca catcgagtgt gccgccgtca ttaagcaccg
    26461 agttttcagc cagaccgccc tcattaacta cctgtgaacc cccattaata atggaacctt
    26521 ccgctatccc gtttgccata atttgttgta ggccagagac gatatcggta ttgatcgcct
    26581 taccataatt ctgaacggtt tgtgtgccgc cattgatgtg cgttttgtca gttgacccac
    26641 catcaacaat ttgttcacca ctttcaatat ttgcctcagt ggctaaacca tataccgttt
    26701 gcttgccacc tttgatattt gctttatcag aagtggcact ggcatatatt gtttgggtgc
    26761 cagcactatt aagtacagtg ccaacatctt ttccataaac atccattttg ccgttggcat
    26821 taataatcgt atcaactgcc cgggaaccag tgacgactgt taatgagcca gcgttttcca
    26881 gcactacatt tttagcttct gaattcctga tgtagaaagc atcaccataa ctgttggttc
    26941 cttcgataag ggttccggaa gttgtggaag caattaatgc gccgccggat tgttgctcaa
    27001 catgcttagc ctcaccaccg tcctgaacct ccagaacgcc accattatta agtctggtgg
    27061 tatctgtttt agcctccttc tggacaatca gcttaccgcc agcatcaacg gtagtatttt
    27121 tcgccgaggt tttagccact accgtcagtt cgccggtatt ttccagcaca acataattag
    27181 cctcccctcc ggtaatagtg aagtgagaga gcttgttgta tccttcaata tcagtccctg
    27241 cgcccgtgtt ggcaactaaa gcactgcccg tctcctggtt aaccccatgt gcaataccgc
    27301 cggtatagac aatcagcgag cctccggcgc taatattgct gccaattgcc gtaccatctt
    27361 tctcaacaac ttgctggctc ccggaggata taattgttgt gtcagctttc ccaccgcttt
    27421 tgatattttg cgttccgctg ttgatattgg tgcctgtggc tatgccataa ttattaagat
    27481 tctgtgtgcc accattaatt atggtatttg tcgcgtttcc tgcaacatcc ataaccccgc
    27541 cattatctat tcgggtcgca tcagctttag cattcgttaa aactgacatt gttcctttat
    27601 ctttaataat cgtcttgttt gcggaatgag atacttctaa atggcctcca ttttccagca
    27661 acacattgtc ttccacttta ttggggatgg agaatgcacc ttcactattc gtaccgctca
    27721 ccgtcgtacc gttagtgttt gtttttaaaa ttgcaccatc gtgctgggta acatttgttg
    27781 ccgtaccacc actaacatca agcacgccac cggaataaac ttcaataaca tccgaggtgc
    27841 tggtgttatc aacaatttgc gtgccaccag aatagatctg cgtaattttt gccgttgact
    27901 tactattcag agactgagtt ccgccttcaa tcgtcgtgtc cagcgcacgg ctctcatata
    27961 ctctttgctc accgccattt ttaatggttg ttgtttctac tgtgctctgt tcaacatact
    28021 gtcgaccacc atttatggtt gtgttcgttg ccagacttcc ttgtactacg tcctgagagc
    28081 cagacttatt tatcgttgta ccatcagcat gcccttgaac ttttactatc tggctaccac
    28141 catcaatgag tattccattc gcactccctc cctctacgcg tgaagcaccg cccttaattg
    28201 tcgttccatt gctgataccc cctttataaa cgtcctgatt gccactctcg attgtcgtac
    28261 ctgtggaaat acccccgtca tgaattgact gtctgccacc gttaatggtt gtattattag
    28321 cctgccccac aaaattgtta tgacttccta tatcttgata tccaccagat tcaataagac
    28381 ttccattaga taccccgcca tgaacattct gctggccatg gttgataata tgagtgttat
    28441 ttgttgtacc tcgttcatcc actttttggt tgccatctac agtctcatcg tttaccacac
    28501 caataacatc aggagtgaag gccgccatac cgggcggggc atatatcaag gcagatatca
    28561 ataaggaaag tactgagcgg cgacaataat ggggactggt cctgttcata aatttcatcc
    28621 tctgaaaagt gaatactgag tagcgtttaa gcgaccttag ctctgctgaa acatcagccc
    28681 acaggcacca gaccagggga ttcatcctga agagacagcg caagtgtatt gtgttcaccg
    28741 ctcatcaaag acatcatgat gaaatgatga tattccacat aagaaagaag cattttttaa
    28801 acgcagtgcg ctgaagtatg gttggataaa aaagtcaatc tattcaggga atacgggggt
    28861 attcttttct ttcgacaatc aggccgtcgg caaaataaaa tgatttacat aatcgtttct
    28921 gatgaatatc ttctgctcac ataaaaatca cacaataact ttgagatcgc agattctttt
    28981 acttttacag cattcgtccc cccattgttg ggcaaatata gattgggcca gagcacgaaa
    29041 gttaatacca cgtttgcaca gctcctccaa cagcacgaca agatgccaca tactgcgccc
    29101 cagtcggttc ggtttacaga ctacccgtgc gtccaccgcc gataatgtcc tgagcagttt
    29161 tttcagtccg gaccacctgc cacggtttta cgttgaagag gtaaccctga gcacgcagtt
    29221 catcagtcag gcgtggtgta ccgtaaccct attattgatg ggtaagatca aaaaaacttt
    29281 caggcagcta aggaaagttg aaccagacat tagaataaat atttcaacca attacagcac
    29341 caattcagac accgccagct caacaaataa atcaaggggt tacgtgaaag cgtagcccct
    29401 ttttctttgg tagtggcagc aaaatggttg tagtgtaaaa aataatcccg tttaatcaat
    29461 caataataca tattgtttca atctacgtta ttatctcttt gtaaaaatag ccatttatta
    29521 atcattgaaa actgctttta gaacttgata caacgggact agtcacaaca ggactattct
    29581 caacgggatc atcctcagag gaactatcat caaagtcatc atccataaat aaaatatcat
    29641 cgaatggtgc cacgcccgtg atgagtttta ttttattatt acgatcagtc aagactccac
    29701 ttaaaccgtt ttcgctcaca ggttttaatg atttttcatt actcttgttg taagcaggcg
    29761 cattaaaaat acacggagta tcaagatcaa acaat
//
LOCUS       DAZKIV010000042        29652 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZKIV010000042 DAZKIV010000000
VERSION     DAZKIV010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.042"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..1879)
                     /gene="glmS"
                     /locus_tag="ACHOT6_001882"
     CDS             complement(50..1879)
                     /gene="glmS"
                     /locus_tag="ACHOT6_001882"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFP9947109.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2041..3411)
                     /gene="glmU"
                     /locus_tag="ACHOT6_001883"
     CDS             complement(2041..3411)
                     /gene="glmU"
                     /locus_tag="ACHOT6_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFP9947110.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3762..4181)
                     /gene="atpC"
                     /locus_tag="ACHOT6_001884"
     CDS             complement(3762..4181)
                     /gene="atpC"
                     /locus_tag="ACHOT6_001884"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFP9947111.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTLLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4202..5584)
                     /gene="atpD"
                     /locus_tag="ACHOT6_001885"
     CDS             complement(4202..5584)
                     /gene="atpD"
                     /locus_tag="ACHOT6_001885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFP9947112.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5611..6474)
                     /gene="atpG"
                     /locus_tag="ACHOT6_001886"
     CDS             complement(5611..6474)
                     /gene="atpG"
                     /locus_tag="ACHOT6_001886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFP9947113.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6525..8066)
                     /gene="atpA"
                     /locus_tag="ACHOT6_001887"
     CDS             complement(6525..8066)
                     /gene="atpA"
                     /locus_tag="ACHOT6_001887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFP9947114.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDCFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8079..8612)
                     /gene="atpH"
                     /locus_tag="ACHOT6_001888"
     CDS             complement(8079..8612)
                     /gene="atpH"
                     /locus_tag="ACHOT6_001888"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFP9947115.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8627..9097)
                     /gene="atpF"
                     /locus_tag="ACHOT6_001889"
     CDS             complement(8627..9097)
                     /gene="atpF"
                     /locus_tag="ACHOT6_001889"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFP9947116.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9159..9398)
                     /gene="atpE"
                     /locus_tag="ACHOT6_001890"
     CDS             complement(9159..9398)
                     /gene="atpE"
                     /locus_tag="ACHOT6_001890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFP9947117.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9445..10260)
                     /gene="atpB"
                     /locus_tag="ACHOT6_001891"
     CDS             complement(9445..10260)
                     /gene="atpB"
                     /locus_tag="ACHOT6_001891"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFP9947118.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10269..10649)
                     /gene="atpI"
                     /locus_tag="ACHOT6_001892"
     CDS             complement(10269..10649)
                     /gene="atpI"
                     /locus_tag="ACHOT6_001892"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFP9947119.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11266..11889)
                     /gene="rsmG"
                     /locus_tag="ACHOT6_001893"
     CDS             complement(11266..11889)
                     /gene="rsmG"
                     /locus_tag="ACHOT6_001893"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFP9947120.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11953..13842)
                     /gene="mnmG"
                     /locus_tag="ACHOT6_001894"
     CDS             complement(11953..13842)
                     /gene="mnmG"
                     /locus_tag="ACHOT6_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFP9947121.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14224..14664)
                     /gene="mioC"
                     /locus_tag="ACHOT6_001895"
     CDS             complement(14224..14664)
                     /gene="mioC"
                     /locus_tag="ACHOT6_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFP9947122.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLL"
     gene            complement(14754..15212)
                     /gene="asnC"
                     /locus_tag="ACHOT6_001896"
     CDS             complement(14754..15212)
                     /gene="asnC"
                     /locus_tag="ACHOT6_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFP9947123.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15364..16356
                     /gene="asnA"
                     /locus_tag="ACHOT6_001897"
     CDS             15364..16356
                     /gene="asnA"
                     /locus_tag="ACHOT6_001897"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFP9947124.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16361..17812)
                     /gene="viaA"
                     /locus_tag="ACHOT6_001898"
     CDS             complement(16361..17812)
                     /gene="viaA"
                     /locus_tag="ACHOT6_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFP9947125.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17806..19302)
                     /gene="ravA"
                     /locus_tag="ACHOT6_001899"
     CDS             complement(17806..19302)
                     /gene="ravA"
                     /locus_tag="ACHOT6_001899"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFP9947126.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNEQPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            19525..21393
                     /gene="kup"
                     /locus_tag="ACHOT6_001900"
     CDS             19525..21393
                     /gene="kup"
                     /locus_tag="ACHOT6_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFP9947127.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            21560..21979
                     /gene="rbsD"
                     /locus_tag="ACHOT6_001901"
     CDS             21560..21979
                     /gene="rbsD"
                     /locus_tag="ACHOT6_001901"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFP9947128.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPISKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            21987..23492
                     /gene="rbsA"
                     /locus_tag="ACHOT6_001902"
     CDS             21987..23492
                     /gene="rbsA"
                     /locus_tag="ACHOT6_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFP9947129.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            23497..24462
                     /gene="rbsC"
                     /locus_tag="ACHOT6_001903"
     CDS             23497..24462
                     /gene="rbsC"
                     /locus_tag="ACHOT6_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFP9947130.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            24487..25377
                     /gene="rbsB"
                     /locus_tag="ACHOT6_001904"
     CDS             24487..25377
                     /gene="rbsB"
                     /locus_tag="ACHOT6_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFP9947131.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            25503..26432
                     /gene="rbsK"
                     /locus_tag="ACHOT6_001905"
     CDS             25503..26432
                     /gene="rbsK"
                     /locus_tag="ACHOT6_001905"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFP9947132.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            26436..27428
                     /gene="rbsR"
                     /locus_tag="ACHOT6_001906"
     CDS             26436..27428
                     /gene="rbsR"
                     /locus_tag="ACHOT6_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFP9947133.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(27394..28821)
                     /gene="mdtD"
                     /locus_tag="ACHOT6_001907"
     CDS             complement(27394..28821)
                     /gene="mdtD"
                     /locus_tag="ACHOT6_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFP9947134.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(28844..29536)
                     /gene="yieP"
                     /locus_tag="ACHOT6_001908"
     CDS             complement(28844..29536)
                     /gene="yieP"
                     /locus_tag="ACHOT6_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFP9947135.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         7124 a         7599 c         7584 g         7345 t
ORIGIN      
        1 tataaaaaag ggccaccggt cggcagccct tttttatcta tgatagtgat tactcaaccg
       61 taaccgattt tgccaggtta cgcggctggt caacgtcggt gcctttgatc agcgcgacat
      121 ggtaagccag cagctgcagc ggaacggtgt agaagatcgg tgcaatcacc tcttccacat
      181 gcggcatctc gatgatgtgc atgttatcgc tacttacaaa acccgcatcc tgatcggcga
      241 agacatacaa ctgaccgcca cgcgcgcgaa cttcttcaat gttggatttc agtttttcca
      301 acagttcgtt gttcggtgca acgacgataa ccggcatatc cgcgtcaatc agcgccagcg
      361 ggccgtgttt cagctcgcct gcagcgtagg cttcagcgtg aatgtaagag atctctttca
      421 gcttcaatgc gccttccagc gcgattgggt actgatcgcc acggcccagg aacagcgcgt
      481 gatgtttgtc agagaaatct tctgccagag cttcaatgcg tttgtcctga gacagcatct
      541 gctcaatacg gctcggcagc gcctgcagac catgcacaat gtcatgttca atggaggcat
      601 ccagaccttt caggcgagac agcttcgcca ccagcatcaa cagcacagtt aactgagtgg
      661 tgaatgcttt agttgatgcc acgccgattt ccgtacccgc gttggtcatc agcgccagat
      721 cggattcgcg caccagagaa gaacccggaa cgttacagat tgccagcgaa ccaaggtaac
      781 ccagctcttt cgacagacgc aggcccgcca gggtatccgc ggtttcgcca gactgtgaca
      841 aggtgatcat caggctgtta cgacgcacgg cagatttgcg atagcggaat tcagaggcga
      901 tttcgacgtc gcacggaata cctgccagcg attcaaacca gtaacgggaa accataccgg
      961 agttataaga agtaccacag gcgaggatct gaatatgctc aaccttcgac agcagttcgt
     1021 cggcgttcgg tcccagctcg cttaaatcaa cctgaccgtg gctgatgcgc ccggtaaggg
     1081 tgtttttgat cgcgttcggc tgttcgtaga tctctttctg catgtagtga cggtaaatac
     1141 ctttatcgcc cgcgtcatat tgcagattgg attcgatatc ctgacgtttt acttccgcgc
     1201 cagttttatc gaagatgttt accgaacggc gagtaatttc cgcaatatcg ccctcttcaa
     1261 ggaagataaa gcgacgggtc accggcagca gcgccagctg gtcagaagcg ataaagtttt
     1321 cgcccatacc cagaccaatc accagcggac taccagaacg tgccgccagc agggtatccg
     1381 ggtgacggga gtccatgatc actgtaccgt acgcaccacg cagttgcggg atagcacgca
     1441 gaacagcctc acgcagagta ccgccctgtt tcagctccca gttcaccaga tgggcaatta
     1501 cttcggtgtc tgtttcagaa acgaaggtat agccacgcgc ttttaactct tcgcgcagcg
     1561 gttcatggtt ttcgatgatg ccgttatgca ccaccacaat gtgttcagaa acatgcggat
     1621 gcgcattcac ttctgaaggt tcaccgtggg tcgcccagcg agtatgagca atacccgtac
     1681 cgccatgcag aggatgttct tccgctgcct gagccagcat ctggacttta ccgaggcgac
     1741 gcaggcgggt catatgacct tccgcatcaa caacggccag accggcagag tcatatccgc
     1801 ggtattccag acgacgtaaa ccttcaagaa ggatttctgc tacatcacgt tgcgcgatcg
     1861 cgccaacaat tccacacata gtttttgatt ccgatttata tcgttgtcgg tcaacctgta
     1921 tgcccgtttt ttcgggcgcc ccgagccttg tagagagtgg ggttattttt atagttactg
     1981 cttgtgggag ggagattatg ttatctcctc atcccagatg accggattac ccggccagaa
     2041 ttactttttc tttaccggac gacgccagcc ttctttctga gtctgcggca cacggctgat
     2101 agctaatgca ttttcgccga cattacgcgt cacagttgta cccgcagcaa tggtcgcgcc
     2161 tttgcctact gttaccgggg ccaccagctg agtgtcggaa ccgacaaaca catcgtcgcc
     2221 gataatggtc ttaaatttat tcgcaccatc gtagttgcag gtaatggttc ccgcgccgat
     2281 gttaacgtta tcgccaattt ccgcatcgcc caggtaagtc agatgaccag ctttcgagcc
     2341 tttacccaga cgcgcttttt tcatctcaac aaagttaccg acgtgtgcac cttccagcaa
     2401 ctcagcacca ggacgcagac gggcaaacgg gccaatagta caggccgctg ccagattcgc
     2461 gtcttccaca acggtatacg gactgatttc gcaatcatcg ccaatcacgc tgtttttaat
     2521 cacgcaaccg gtgccgattt tcacgcgatg accgagagtc acgttgccct cgatgataac
     2581 gttagtatca atttcaacat cgcgcccgtg agtaagcgta ccgcgcagat caaaacgcgc
     2641 cggatcgcgc agcataacgc ctgctaacag cagtttttca gcctgttcgg actggtaaac
     2701 gcgctccaga cgggagagtt gcaggcggtt attcacacct tctacttcgc ttaaacgttg
     2761 cggatgaacg gcgacgattt cacgcccttc ctgatacgcc agcgcaataa tgtcggtgat
     2821 gtagtattcg ccctgagcat tattgttggt cagcttcgcc agccagcgtt tcatatctgc
     2881 gccgttagcg atcagaatgc cggtgttgat ctcctgaatc tgacgctgct cgtcggtggc
     2941 atctttgtgc tcaacaatgc cggtaacttt gccattttca cgggtgatac gtccataacc
     3001 ggtcggatca tccagtttca ccgtcagcag accaatgcca ccctgcggtt tagcatcacg
     3061 caggcgctgg agtgtttcga cagagatcag cggcacgtcg ccgtagagca ttaaaatgtc
     3121 ttcatcatcg gcaaagaaag gtgcggcctg ctgcattgca tgacccgtac ccagctgctc
     3181 tgcctgaagc acccagttaa ggttgtcgtc tttcagcgcc tgtttaagca gatcgccgcc
     3241 gtgaccgtac accaggtgaa cgtgcgctgc gcctaattca ttcgcagcat caatgacatg
     3301 ctgaaccatc gctttcccgg caagggtatg cagcactttc ggaagatcgg aatacatgcg
     3361 cgtgcctttg cctgcggcaa ggatcactac gctcatagca ttattcaaca tacgcgtcct
     3421 gactgtaatt tgagaacgaa tttaaaccgc ttcaccttga aaaaactaca tttttttcat
     3481 cgtgaaatgg acagaggata aattgttcaa taacggatta tccccgctga cgaaagtgcc
     3541 attttcgacc atcgtcacgc cttttgtctg cagaaaatta aggcagtacc ttcatttaag
     3601 cgtcaggtgg atgtttttgc tcttatttcg atcaatgaat aaacgggaaa acaggggtgt
     3661 tttatatcag caggatctat gtgaacgctc ttcaggacgg atcacgcgta aaaaaaagcc
     3721 agcctgtttc cagactggct tttgtgcttt tcaagccggt gttacatcgc ttttttggtc
     3781 aactcgataa cgcgcagctg cgcgatcgct ttggccagtt ccgcagacgc ctgagcgtaa
     3841 tctacgtcgc cgtgagagct gctaatgtgc tcttcagcct tacgtttcgc ttccatggct
     3901 cgcgcttcgt cgagatcctg gccgcgaatt gcggtgtcgg ccagaagggt cacgttgcca
     3961 ggctgcactt caagaatgcc gccagacaga tagataaact cttcgtgacc gtgctgtttc
     4021 acgatgcgaa tcataccagg cttaatggcg gtgagcagcg gtgcgtggcc agggtagatc
     4081 cccagttcac cttcgctacc cgttacctgg attttctcga ccagaccaga gaacatttgt
     4141 tgctctgcgc tgacgacgtc caggtggtaa gtcattgcca tatcaccctc cgattaaggc
     4201 gttaaagttt tttggctttt tccacagctt cttcgatgga accgaccatg tagaacgcct
     4261 gctccggcag gtgatcgtat tcgccttcca tgatgccttt aaagccacgg atggtgtctt
     4321 tcagggagac gtatttaccc ggagaaccgg tgaatacttc tgccacgaag aacggctggg
     4381 acaggaagcg ctggatctta cgagcacgcg ctaccaccag tttgtcttct tcagacagtt
     4441 catccatacc caggatggcg atgatgtctt tcagttcctg ataacgttgc aggatggact
     4501 gaacgccacg cgcggtgtcg tagtgttcct gaccaaccac cagcgggtcc agctgacggc
     4561 tggtggagtc cagcgggtca acggccgggt agatacccag agacgcgatc tgacggctca
     4621 gtaccacggt tgcgtcaagg tgcgcaaagg tggttgccgg agacgggtca gtcaagtcat
     4681 ccgcaggtac gtataccgcc tgtacggagg tgatagaacc agttttggtg gaggtgatac
     4741 gttcctgcag aacgcccatc tcttccgcca gggtcggctg ataacctacc gctgaaggca
     4801 tacggcccag cagtgcggat acttccgtac cggccagggt gtaacgatag atgttgtcaa
     4861 cgaacagcag aacgtcacga ccttcgtcac ggaatttctc agccatggtc agaccggtca
     4921 gagcaacgcg cagacggttt cccggcggct cgttcatctg gccatacacc agggatactt
     4981 tgtcgataac gttggagtcg gtcatttcgt ggtagaagtc gttaccctca cgagtacgtt
     5041 cacctacgcc cgcaaacaca gagtaaccgg agtgctcgat cgcgatgtta cgaatgagct
     5101 ccatcatgtt tacggtttta cctacacccg caccaccgaa cagaccaact ttaccgccct
     5161 tagcgaacgg acacatcagg tcgataactt tgataccggt ttccagcagt tcctgagagt
     5221 ttgacagctc ttcgtaggaa ggtgctgcgc ggtgaatcgc ccaacgctct tcttcaccga
     5281 tctcgccttt catgtcgacc ggttcaccca gtacgttcat gatacggccc agagtcgctt
     5341 tacctaccgg gacttcaatc gggtgttcga ggtcttttac atccagaccg cgacgcagac
     5401 cgtcggagga acccattgcg atggtacgta cgataccgcc gccgagctgc tgctgaactt
     5461 ccagcaccag acgctcatta ccattttgca cctcaagagc atcgtacacg cgcggtacgg
     5521 catcctgagg gaattcgacg tcaactacgg cgccgattac ctggacaatc tttccagtag
     5581 ccatcttaaa tcctctacga aataacctgt ttaaaccgcg gcggcccccg agacgatctc
     5641 ggtgagttcc tgagtaatgc tggcctgacg agctttgttg tataccaact gcagctcttt
     5701 aatcaggctg ccgccattgt cggtcgcggc tttcatcgcc accatacggg cggcctgctc
     5761 gctggccagg ttttcaacca cgccctgata aacctgagat tcgacataac gacgcagcag
     5821 ggtatccagc aacgccttcg gatcgggttc gtacaggtaa tcccaggatt tatgtttcag
     5881 atcatcatca tctgatgccg gtaacggcag cagctggctg atggtcggaa cctgagacat
     5941 ggtgttaata aatttgttgc tgacaatgta aagcttgtcc agacggcctt cgtcgtaggc
     6001 ctgcaacatc acttttaccg gaccgatcag ttcggacagg gaagggttat cccccatgcc
     6061 ggtgacctgg gcaacaacat tgccgcccac ggagttgaag aacgacacgc ctttcgagcc
     6121 gatcattgcg aggtcgcatt gaacgccttt gtcggtccag gtcttcattt ccgccagcag
     6181 ttttttgaac aggttaatgt tcaaaccacc gcacaaacca cggtcggtcg acaccaccag
     6241 gtagcccacg cgtttaacgt cgcggtcttc caggtaaggg tgcttatatt ccagattacc
     6301 gtgtgcaagg tgaccaatca ctttgcgcat ggtttctgca taaggacggc tggccgccat
     6361 gcgatcctgc gatttacgca ttttggaagc ggcgaccatc tccatcgctt tagtgatctt
     6421 ttgcgtgttc tggacgcttg cgatcttact acgtatctct tttgcgccgg ccatgagctt
     6481 ctcctcaatg ccttgcggcc tgccctaagg caagccgcca gacgttacca ggattgggtt
     6541 gctttgaagg aatcgaggat gcctttcagc ttgccttcga tttcgtcgtt gtagccaccg
     6601 gtctggttga tctcttgcat caacggagcg tgatcacggt cgacgtaagc cagcagagcg
     6661 gcttcgaagc tgccaatttt cgacagttca acatccgcca ggtaaccacg ttctgctgcg
     6721 aacagaacca gagactgctg cgcaacggac atcggcgcat actgtttctg tttcagcagt
     6781 tcggtcactt tctgaccgtg gtcaagctgc ttacgtgttg catcgtcaag gtcggatgca
     6841 aactgagaga acgctgccag ttcacgatac tgtgccagag cggtacggat accaccggac
     6901 agttttttca tgatcttggt ctgtgctgca ccaccaacac gggatacgga aatacccggg
     6961 ttaaccgcag gacgaatacc ggcgttgaac aggttggttt ccaggaagat ctgaccatcg
     7021 gtaatggaga ttacgttggt cggaacgaac gcagaaacgt cacccgcctg agtttcgata
     7081 atcggcagtg cggtcagaga accggttttc cctttcactt cacctttggt gaaggcttca
     7141 acgtattcgg cgttaacacg tgcagcacgc tccagcagac gagagtggag gtagaaaacg
     7201 tcgcccggga atgcttcacg tcctggcgga cgacggagca gcagggagat ctgacggtaa
     7261 gcaacagcct gtttagacag gtcatcgtaa atgatcagcg catcttcacc gcggtcacgg
     7321 aagtattcgc ccattgcgca accggcatac ggtgccaggt attgcagtgc agcggattca
     7381 gacgcggttg ctaccacaac gatggtgtta gccagtgcgc cgtgctcttc cagtttacgt
     7441 accacgttag aaatggtgga cgctttctgg ccgatagcga catagataca tttgataccg
     7501 gaatcgcgct ggttgatgat ggcatcgata gccagtgcgg ttttacctgt ctgacggtca
     7561 ccaatgatca attcacgctg accacgaccg attgggatca tggagtcaac ggctttataa
     7621 ccggtctgta ccggctgatc tacggactga cgttcgataa cgcccggagc gattgcttct
     7681 acagcagaga agcagtcgtg atccagcgga cctttaccgt cgattggtgc acccagagtg
     7741 ttaaccacac ggcccagcag gccacggcca accggaactt ccaggatacg gccagtacac
     7801 ttaactttca tgccttcggc aaggtcagcg tacggaccca taacaaccgc acctacagag
     7861 tcgcgctcga ggttcagtgc gatagcgtaa cggttacccg gcagggagat catttcaccc
     7921 tgcatacaat cggccaggcc gtgaatgcgg ataacaccgt cacttacaga aacaatagta
     7981 ccttcgttgt gagcttcact cacaacattg aactgagcaa tgcgctgctt gatcagttcg
     8041 ctgatttcgg tggaattcag ttgcatgctc cagtcccctt aagactgcaa gacgtctgca
     8101 aggcgctcaa gacgaccgcg tacgctgcca tcaatgacca tatcacccgc tcggatgata
     8161 acgcctgcca ttacagactt atcgattttg caattcagct taactttgcg tgacagacgt
     8221 ttttccatcg cagcagaaat tttcgcgagc tgttgttcac tcagtgcggc agcggaaatg
     8281 acgtctacct cagcggtagc ctcactcacg gcacgaaggt gaataaactg ctccagaaca
     8341 tccgggagcg cgttaagacg accattttca gccattaccc gaatcaggtt ctgaccgttt
     8401 tcgtccagtt gctcaccaca aactgcgata aacgactcgg cgagcgtttc tggcgcaagc
     8461 gcgccagaga gaagctctgc catttgttcg tttttggtta cctcggcggc aaacgccagc
     8521 atgtcctgcc agcgttctac actttggtgt tcgacggcaa agtcaaaagc tgctttggcg
     8581 taggggcgag ctaccgtaat aaattcagac atcagcccct ccctccttac agttcagcga
     8641 caagtttatc cacgatgtcg ctgttagcag cttcatccac ggaacgttcg atgatcttct
     8701 cggcgccagc aacagccagg atagcaactt gcttacgcag ctcttcacgg gcacgtttac
     8761 gctcggcttc aatttctgcc tgcgcctggg ccacgatttt agtacgttcc tgttctgcct
     8821 cagctttcgc ttcgtccaga atctgcgagc ggcgtttgtt cgcctgctcg atgattacct
     8881 gggcttccgc tttcgctttt ttcagctggt cggtcgcgct ggcctttgca aggtcaaggt
     8941 ccttatgtgc tcgttctgcg gaagcaaggc cgtcagcaat ttctttttga cgtttttcga
     9001 tggctgccat taatggcggc catacgtact tcatgcagaa cagaacgaac aggacaaacg
     9061 cgatggcctg gccgaggatt gttgcgttaa gattcacagc acaatgcctc tatttagtta
     9121 acgttctgat attgctcttt aaataaaagc aacgcttact acgcgacagc gaacatcacg
     9181 tacagaccca gacctacagc gatcatcggg atagcatcca ccagacccat aacgataaag
     9241 aactgagtac gcagcagagg aatcagatca ggttgacgcg ctgcgccttc caggaattta
     9301 cccccgagga tgccgatacc gatcgcagca ccgattgccg ccagacccat catcacagcg
     9361 gcagccatgt acagcagatc catattcagg ttttccatga cagtctccag tttgtttcag
     9421 ttaaaacgta gtagtgttgg taaattaatg ttcttcagac gccatcgaca gatagacgat
     9481 cgtcagaacc atgaagatga aggcttgcag cgtaatgatc aggatgtgga aaatggccca
     9541 cggcacattc aggatccact gtgaccacca cggcaacaga ccagcaatca gaatgaaaat
     9601 cagctcaccg gcatacatgt taccgaacag tcgcaaacca agtgaaactg gtttggacag
     9661 caggcttacc ccttcaagga ttaagttgac aggaatgaac gcccagtgat tgaacggctg
     9721 cagcgtcaac tctttcgtga agccgccgat gcctttcatt ttgatgctgt agaacagaat
     9781 caggataaat acgcccaatg ccatagacag cgttacgttc acgtccgcag acggaaccac
     9841 acgcagtgca ggcagaccca gtacatgttc agcaatgtac ggcagcaggt cgataggcag
     9901 taaatccatc aggttcatca ggaataccca gacgaagatc gtcagggcca gcggagcaat
     9961 cagcttgctt ttgccatggt acatgtcttt cacgctacca ttaacaaagc cgatcaccag
    10021 ctcaatcgcg gtctgaaact tacctggcac accgctggtc gcctttttgg ctacgctacg
    10081 gaataaaacc aggaacaaca gacccagcac caccgagaag aacatggagt caatattgat
    10141 tgtccagaag gtggctgggg ggttttgtgg atccaccagc gagaatgtac gcaggtccag
    10201 ctgaaggtta ttcaggtggt gtcctatgta atcctgcggc gtcatatttt ctgaagccat
    10261 gatgcctttt accctttgtt gttaattaca gccggtgcca gtatctgaac caccagcacc
    10321 aaaacccacg taacgatcag cggcaagaat accgccttta aaaccgccaa cgccaccacc
    10381 agtaacacca acatcgccag aactttgaaa gcttcgccaa atgcgaatgt ccaggccacc
    10441 cggcctttcg ctggtgtatg cgcctggtga cgccaggcaa atatcataaa caaaacgtta
    10501 ggcagaaaga ctgccaggcc cccgcttatt gcagagacgc cccagaaggg gtctttgagg
    10561 ctgaacagca atccacttgc tatcaccacc agtaactgaa cgagcagaag cttccgagca
    10621 acgtttcgac tcacgagcga cacagacatc acgtttttca ctcctgctcc cttcgaggta
    10681 tgccgcgtgt cgtataaaac tttctttaag gcttagagtc aagcatcaaa aagcggtcaa
    10741 attatacggt gcgcccccgt gatttcaaac aataagtagc caaaaggtga ataaatgttt
    10801 aaatattttt ccagtgcata caattgcgac ttttctgcta accctgttcg atcatgaaaa
    10861 actgtaaata acgcgtaaac actggtgata aagcgtgctt cagatcacat attgcgcatg
    10921 ttcgcgcaca gcatatttat ttacttggca aatgatgcct ttgcaagttt atgatatttc
    10981 agtctaaaaa cagatactgt tttaataaat gacatttaca caacaaaaac cacccattga
    11041 catttttaat aatgttttaa cagccaatga tggttcttag cgccgatttt tagcagactg
    11101 atattttcac taatgactta ttttctgctt accaaaaaaa gccacgttat cttgttgatg
    11161 caaaagagtg aacgtggcgt taaatgtaac cagttatatc agtagaaaac ctggttgttg
    11221 ttaacagtct aaccggtcaa ttttttatga tttttttgat aaaaattaaa ttttatttgc
    11281 tttaatcacc accagatgac gttcgccatc cagggctgga acctgaagtt taaccactga
    11341 ttcgacctga tattcttcgg gcaacaaagc gatttcatct tccggcattt gccctttcag
    11401 cgcgtaaaaa cggccttgct caccaggaag atggtggcac cagctcacca tatcgttcag
    11461 agaggcaaaa gcgcggctaa ttacgccatc aaatggcggc tctgaaggaa actcttctac
    11521 cctgctctgt actggttcaa tattctccag tttaagctca tgttgcacct gacgaaggaa
    11581 acgcacgcgt ttaccaaggc tatccaacag agtgaaatgg gcttcaggac gcacgataga
    11641 gagtggaatg cctggcagtc ctggtccggt gccgacatcg ataaaccgtt caccttgcag
    11701 atacggtgcc accacaatgc tatcgagaat atggcgtacc agcatctcat taggatcgcg
    11761 gaccgaagtc aggttgtacg ctttgttcca tttatgcagc atattcacgt aggcaataag
    11821 ctggtttttc tggtgatcgg taagcgaaat acctgcgtct ttcagcagta aggagagttt
    11881 gttgagcacg gtgattacct gttcttgatg cgttgcctgg taagcgggtg cttaccaggc
    11941 atttttaatg cgttatgcgc tacgacgcag cataccctgt tttttcagcc acaccagcag
    12001 aatggagatg gccgcaggcg tgacgccaga aatacgcgaa gcttggccga tagaggctgg
    12061 tttgtgatcg ttaagtttgg cgatcacttc gttagaaaga ccggatacct ggcggtaatc
    12121 cagtgtcgcg ggtagcaggg tgttctcgtt acgcagctgc ttttcgatct catcttgctg
    12181 gcgcgcgata taaccttcgt atttaacctg aatctcaacc tgttccgccg cctgttcgtc
    12241 tgtcaacgca ggggcaaacg gcgtcagcgt ggttaatttt tcataagtca tttccggacg
    12301 acgcagcaga tcttcaccac tggcttcacg ggaaagcggc gcagtcaggt gagcattcac
    12361 ttcggctgca gcttccgccg acggggttac ccaggtcgat ttcagacgct gacgctcacg
    12421 ctcgatattc tcaagtttct cgttaaagcg cgcccaacgt tcgtcatcca ccaggcccag
    12481 ttcacgaccg atttcagtca aacgcagatc cgcattatct tcgcgtagca tcagacgata
    12541 ttctgcgcgc gaagtaaaca tacgatacgg ttctttggtt cctaaagtgc acaggtcatc
    12601 aactagtacg ccgagatacg cctgagaacg tgccggagcc caaccttctt tgtcagcaga
    12661 cagacgggca gcgttaagac cggccagcaa accttgcgca gcggcttctt cgtaaccggt
    12721 agtgccgtta atctgaccag caaagaacag cccctggata aacttgctct ccagcgtcgg
    12781 tttcaggtcg cgaggatcga agaagtcata ctcaatggca taacccggac gcacgatctt
    12841 cgcgttttcc atcccctgca tagagcggac gatttgcatc tgcacatcga acggcaggct
    12901 ggtggagata ccgttcggat aaatttcatt agaggtcagt ccttccggtt caaggaagat
    12961 ctgatgctga tttctgtcgg cgaagcgcat gactttgtct tcgatcgacg ggcagtagcg
    13021 tgggccgaca ccttcgatca cccctgcata cattgggcta cgatcgaggt tactgcggat
    13081 cacatcatgg gttttctcgt tggtatgagt gatataacac ggcacctgct ggggatgctg
    13141 ggacgcattg cccataaacg agaataccgg cattgggtta tcgccatgct gttgcgccag
    13201 cacactaaag tcgatagttc gagcatcaat acgcggtggt gtcccggttt tcagacgacc
    13261 aacgcgcagc ggcagttcac gcaaacggcg agaaagcgga atggacggcg gatcaccagc
    13321 acggccaccg ctgtaattat ccagaccgat atgaatttta ccgtcgagga acgtcccaac
    13381 ggtgagcacg acggctttgg cacggaactt cagtcccatt tgggtaacag caccgaccac
    13441 gcgatcgttt tcgacaataa gatcttcaac cgcctgctgg aagatcatca ggttcggttg
    13501 gttctccagc gccgtacgta ccgcctgacg gtagagcaca cgatccgcct gagctcgggt
    13561 agcgcgaacc gccggtcctt tgcttgcgtt tagtatccta aactggatac ccgcttgatc
    13621 gatcgctttc gccatcagac cgccgagtgc atccacttct tttaccagat gtcccttccc
    13681 aataccgccg atcgccgggt tgcagctcat ctgccccaga gtgtcgatat tgtgtgtcaa
    13741 aagcagagtc tgttgaccca tacgcgccgc ggccatcgcg gcctcggtgc ctgcatgacc
    13801 cccgccaatg atgatgacgt caaaaggatc cggataaaac atggtgattg cctcgcataa
    13861 cgcggtatga aaatggattg aagcccgggc cgtggattct actcaacttt gtcggcttga
    13921 gaaagacctg ggatcctggg tattaaaaag aagatctatt tatttagaga tctgttctat
    13981 tgtgatctct tattaggatc gcactgccct gtggataaca aggatccggc ttttaagatc
    14041 aacaacctgg aaaggatcat taactgtgaa tgatcggtga tcctggaccg tataagctgg
    14101 gatcagaatg aggggttata cacaactcaa aaactgaaca accgttgttc cttggataac
    14161 taccggttga tccaagcttc ctgacagagt tatccacagt agatcgcacg atctgtatac
    14221 ttattagagt aaattaatcc acgatcccag ccattcttct gccggatctt ccggaatgtc
    14281 gtgatcaaga atgttgatct tcagtgtttc gcctgtctgt tttgcaccgg aatttttgag
    14341 ttctgcctcg agtttatcga tagccccaca aaaggtgtca tattcacgac taccaatacc
    14401 gattgcgcca aagcggaccg cagaaagatc gggcttctgt tcctgcaatg cttcatagaa
    14461 aggagaaagg ttgtccggaa tatctccggc gccgtgggtg gagctgataa tcagccagat
    14521 ccccgaggca gataaatctt ccaacagcgg accgtgcagc gtttcggtgg taaaacccgc
    14581 ctcttccagc ttttcagcca ggtgttctgc tacatattcg gcaccgccga gggtgctgcc
    14641 gctgataaga gtgatatctg ccataaaccg ccacctttat taagagtggc gtattgtacg
    14701 ctgtgaacgc gttgggatct acctgtggaa aagtatggga ttaaaaaagc cgatcagggc
    14761 ttgatagtac gcatgatcgg gttttgcagg acgatcaatg tctcggtgga ctgaatttca
    14821 tcaattgttt ggatcttgtt gataagtaca tgctggagag cgtcgatcga acggcacatt
    14881 acttttataa agatgctgta gtggcctgtt gtgtaatagg cttcagtgac ttcatcaagg
    14941 ctttccagct ttgccagcgc ggaagggtag tctttggcgc tctttaatat aatgccgata
    15001 aagcagccta cgtcataacc gagctgcttc gggctgacat caatacgcgc cccggtaatg
    15061 atccccgcct gcttcatttt ctctactcga acgtgaatcg tccccggact gacgccaaat
    15121 tgtttcgcca gttcggcgta agcggtgcgc gcattgccca ttaatgcttc caggatgcca
    15181 cggtccagat tgtcgatcag ataattttcc ataggatttt cttatgcaga ttgatgattc
    15241 attctatttt agccttcttt tttaatgaat caaaagtgtg ttaggctttt tattgaatga
    15301 ttattgcatg tgtgtcggtt tttgttgctt aatcataagc aacaggacgc aggagtataa
    15361 aaaatgaaaa ccgcttacat tgccaaacaa cgccaaatta gcttcgtgaa atctcacttt
    15421 tctcgccaac tggaagaacg tctggggctg atcgaagtcc aggcgccgat tcttagccgt
    15481 gtgggggatg gcacgcagga taacttgtcg ggctgtgaaa aagcggtgca ggtaaaagtg
    15541 aaagctctgc ctgatgccca gttcgaagtg gttcattcac tggcgaagtg gaaacgtcag
    15601 accttagggc aacacgactt cagcgcgggc gaagggctgt acacgcacat gaaagccctt
    15661 cgccccgatg aagagcgtct ttctccgttg cactcggtct atgttgacca gtgggactgg
    15721 gaacgcgtaa tgggcgacgg tgagcgtcaa ttctcgactc tgaaaagcac ggtagaggcg
    15781 atctgggcgg gaattaaagc aaccgaagct gcagttagcg aagagtttgg tttggcaccg
    15841 ttcctgccgg atcagatcca cttcgtacac agccaggagt tactgtctcg ttatccggat
    15901 ctcgatgcca aagggcgtga gcgggcgatt gcgaaagatc ttggcgcggt attcctcgtc
    15961 gggattggcg gcaagctgag tgatggtcat cgtcacgacg tgcgcgcacc ggattatgat
    16021 gactggagca ccccgtcaga gctgggccat gcgggtctga acggcgatat tctggtatgg
    16081 aaccctgtac tggaagatgc gtttgagctt tcttccatgg ggatccgcgt ggatgccgac
    16141 acgttgaagc atcagttggc gctgaccggt gatgaagatc gtctgcagct ggaatggcat
    16201 caggcgctgc tgcgcggtga aatgccgcag accatcggcg gcggtatcgg ccagtctcgt
    16261 ttgaccatgc tgctgctgca actgccgcat atcggccagg ttcagtgtgg agtatggcca
    16321 gctgctgttc gcgagagcgt cccttctctg ctgtaataat ttatcgccgc cagcgtctga
    16381 gcaggcggct tcgcatcccg gtatcaaagc gccagatatg atcgaaaatg cgcatgatgc
    16441 cgggtttgcc gtgtgccgac atcgccacgg catgaaagcg atgctgatgt acccgctgca
    16501 gctctttcac tttactcgtc acgtcgtcag gcaaccgctg agcgataaaa tcagaaatca
    16561 ccaccgcatc ggcatcaaac cattccctgc tttgcaagcg ttccataatg gcgcgaaaac
    16621 aactggcaag atcggtgccg ccacgaaact gctggcttaa aaaacggatt gcttgttcga
    16681 tgccttgtgg gcctgaaagc tcataacgga cgatctcggt ggaaaatagc ataatatagc
    16741 agcgtcggtt ttctgcgaga gcaatgcgca tcaaggccag gcagaacgct ttcgcacact
    16801 gttcattaaa gccgcccatt gagccggaag tatccacaca gacaataaac ggcccgcgcg
    16861 gctgttcatc gtaatcttta tgtaccaccg gacgttcgat cactttttca cgccacgact
    16921 caccgtgcag gcgataggtg agcaactgtt tttccaccag ccgacggtaa aactcatact
    16981 ccagttccgt tatccctagt gtcgccagtt ctggcggcag gagacgtaaa atatcatcgc
    17041 tttgttgcag accatcaacc tgctcaggaa ccgtcgccgg ttcgcgcacc atggtgcgga
    17101 aggtttccat ctgcgcatcg ttgcgcggta ttgatttggc ttcccgagaa cgccccagct
    17161 gctctgccag gcgtttcagt tccggctgtt cgttaagaaa ttcaccgtat ttcacaatca
    17221 actgatagtc gccacgttta agctggccgg cgctcatatc ccacagacga ccagctgcgg
    17281 tattgttatc tgcgagaatc ggttcaagtt gtccgctcag cgtcatgcgt tcctgaactt
    17341 cacttaacag ttgttcgcgt tcttcttcta atagctgttg atttaacgtc gttgcttgca
    17401 cgatcagact taaacgccag cgctggagaa aaagcgtgtg taacgctgaa gtgatcgtgc
    17461 tgttagcatc aaccaactgt cgggcttgtt ctgcccatgg agaattcaga cgatgcagta
    17521 agtccaggat ctgtggtagc tgcacaataa actgcggcgt ggagaggagc tggctttgct
    17581 gatagcacat cacctcttcg gtgagttccg gcgggactcg ggcatctttc agccgactgc
    17641 gcagtgcctc acgccagcgg ggaacatcat cagtgattgc cgccttcagt cgtgggaatt
    17701 tctcaaagaa gactgccagc tgcggtgagg ccagcagcgc gatgatcatc tcttcgatca
    17761 atccctcttc gctgacggcc agcatcacat taagcgtatc cagcgttagc attgttgtgc
    17821 ctggcgaatc tgtgcaccga catcctgtag gctggcttca atgcgaccta accagtcgcc
    17881 agggataaac agacattttt gctgttcgct gaataaagcg tgttgcttgc gccagtcgct
    17941 ttcaagctct tccagttgtt gttttatttc accaggcaga ccttcagccg atgaaccggg
    18001 aagtgccagc gtactgcctt gtaaacttac atcgcgaacg acaagatgtt gggcgctatc
    18061 aacttccaga ttcagtttct gggcaaagcc gataccgttc agtttaccgc gaatttcacc
    18121 acctttgctc agccactgtt ccagcgcgct acgctcaaag gagatatgaa ccacttccat
    18181 atcatgcagt tttaacggtt tttgcagcag tagagtcaga gtggaagcag taacgttaac
    18241 aggaagttga tactgctgac gacggctgaa aatgccgccc agacgaatta ccgttaaggc
    18301 tgttttatcg ctttgttgct gctggagttg caggtgacgt tgcacaatcg cgccaaggcg
    18361 agtcaacatc ccttgctgtt gccaggcgtg accggtcatc aatacatcaa tttgttgttg
    18421 tatcaaattc aggctttgcg cgtcatacca caggcaatct ttcagcaaaa tgagatctac
    18481 cggggcaaca gcactgcgac cgctaaaaaa ggcgctggcc tgcaataatc ggatcgcttt
    18541 tttccagcga cgatccgaga cataaggcgc atccggtaat ttatccagtt gctggcgcag
    18601 cataaaaatc agctcaaata catgatcggg cagcgtaatt tcaccaatct ctttctgcca
    18661 gcgttcatat tcttcatctg tgacctgcaa ggcatcagga accggattgt cgttttcatc
    18721 ctgttgactg gtcagcatgg agcggaaatt cgctttatcc tgcactttat ctaaccacag
    18781 acgaatcagc atgcggtcat ataacgcttc cagactgctg tctgcttccg gctgctcgtt
    18841 ggaggccgcc accagcagac gcatcgggat tttttctacg tgtgcaccgt tgcggaactg
    18901 gcgctcgtta atggcggtga gcaaggtatt aagaattgcc gggcccgctt tccagatctc
    18961 atccagaaag acaatttctg cttccggcag gtaaccgctg gttaaacgtt catagcgccc
    19021 ttcatctttt agcgcctgaa tagaaagggg accaaaaact tcttccggcg tggagaagcg
    19081 ggtcatcaga tattcaaacg cgcgggcatt ctgaaaggcg aattttaagc gccgggcgat
    19141 caaacttttg gcaatacctg gcgggccaag gaggaacaca ctttcaccac ttaatgccgc
    19201 taataaacac aagcggatgg cgtggctacg ttcataaagc cccttttcca acgaactgct
    19261 caggcgggaa attctttccg ctaataaatg agggtgagcc ataatgaagt ggcgtccttt
    19321 cgtcaaaagt tctgcgtaaa ttgcgagtat agacgtttct tgctggtggc taaaatagtc
    19381 tcaaaggggg ggtatttttc tttgagccag gttaatgtgg ccgcatttag gagtacgatt
    19441 ttgccgttaa tcgtgcatac tgtgcgcttt tttgtgggcc aagggactaa gcacacattt
    19501 catatttcaa cgaaagacta gtctatgagc actgataata agcaatcatt gcccgcgatt
    19561 accctcgcgg cgattggagt tgtctacggc gatattggta ccagcccgtt atatacactt
    19621 cgtgaatgtt tgtccggcca gtttggtttt ggcgttgaac gcgatgccgt gtttggcttt
    19681 ttatcgctga tcttctggct gctaatcttt gtggtttcca ttaaatatct caccttcgtg
    19741 atgcgggcag ataacgccgg tgaggggggg atcctgacgt tgatgtcgct tgccgggcgt
    19801 aatacgtcgg cgcgaaccac atcaatgctg gtgattatgg ggctaatcgg cggcagcttt
    19861 ttctatggtg aagtcgtcat aacacccgct atttcggtga tgtcagccat tgaaggtctg
    19921 gaaatcgtcg ccccgcagct ggatacctgg atagttcccc tctcaattat cgttctcact
    19981 ttattattta tgattcaaaa acatggcacg gctatggtcg gtaagctgtt tgcgccgatc
    20041 atgctgacct ggtttttgat tctggcagga ctggggttac gtagcattat tgctaacccg
    20101 gaagtgctgc atgcgctgaa tccaatgtgg gcggtgcatt tcttccttga atacaaaacg
    20161 gtttctttta ttgcattagg ggcagtggtg ttgtcgatta cgggggtcga ggcgctgtat
    20221 gctgatatgg ggcactttgg taagttccct attcgcctgg cgtggttcac cgtcgtatta
    20281 ccttccttga cccttaatta cttcggtcag ggagcgctgt tgttaaagaa cccggaagcg
    20341 attaagaacc cgttcttcct gttggcaccg gactgggcgc tgatcccgct gctgatcatc
    20401 gccgcactgg cgacggtaat tgcctcgcag gcggttatct ctggcgtctt ctcattgacg
    20461 cgtcaggcgg tacgtctggg atatttatcg ccgatgcgca ttattcacac ctccgaaatg
    20521 gagtcagggc aaatctatat tccgtttgtg aactggatgc tctatgtcgc ggtcgtgatt
    20581 gtgattgtca gctttgagca ctccagcaac ctggcggcgg cgtacgggat tgcggtgacc
    20641 ggaaccatgg tgctgacgtc tattctctcg actaccgtgg cacgtcagaa ctggcactgg
    20701 aataagtatt ttgttgcgct gatcctgatt gctttccttt gtgtcgatat tccattgttc
    20761 accgctaacc tcgataaact gctctccggc ggctggttgc cattgagcct cggtacggtg
    20821 atgtttatcg tgatgaccac ctggaagagc gagcgtttcc gcttgctgcg gcggatgcat
    20881 gaacatggta actctctgga agcgatgatt gcttcgctgg agaaatcacc gcctgttcgc
    20941 gtgcccggga ccgcggtgta tatgtcgcgt gcaatcaacg tcattccctt tgcgctgatg
    21001 cataacctta aacataacaa ggtattgcat gagcgggtga ttctgttaac tctgcgtacc
    21061 gaagacgcgc catatgtcca taatgtccgt cgggtacaga ttgaacaact gtcgcccact
    21121 ttctggcgcg tggtggcaag ttatggttgg cgagaaacgc caaacgtgga agaagttttc
    21181 caccgctgcg gtctggaagg attaagttgc cggatgatgg aaacctcctt ctttatgtcg
    21241 catgagtcgt tgatcctcgg caaacgcccg tggtatttgc gtctgcgcgg caagctgtac
    21301 ttgctgctgc aacgtaatgc gctgcgtgca ccagatcaat ttgaaatccc gccaaacagg
    21361 gttatcgaac tgggtactca ggtcgaaatc taacgccaga cgcctccttt ctccataagg
    21421 gggcgttttt gttttcatgg ttaatcacca tgtaaaacgt ttcgaggttg atcacatttc
    21481 cgtaacgtca cgatggtttt cccaactcag tcaggattaa actgtgggtc agcgaaacgt
    21541 ttcgctgatg gagaaaaaaa tgaaaaaagg caccgttctt aattctgata tttcatcggt
    21601 gatctcccgt ctgggacata ccgatacgct ggtggtgtgt gatgctggtt tacccatctc
    21661 caaaagtaca acgcgtatcg atatggcatt aacccagggt gtaccttctt ttatgcaggt
    21721 gctgggcgtc gttacaaatg aaatgcaggt cgaggctgcc attatcgcgg aagagatcaa
    21781 acaacataat ccgcaactcc acgaaacgtt gctcactcac cttgagcagc tgcaaaaaca
    21841 ccagggaaat accattgaaa ttcgttacac cacgcatgaa caattcaaac aacaaaccgc
    21901 agaaagtcag gcggtaattc gcagcggaga atgttctccg tatgcgaata tcattctctg
    21961 tgctggcgtg acgttctgag gccgtcatgg aagcattact tcagcttaaa ggcatcgata
    22021 aagccttccc gggcgtaaaa gccctctcgg gcgcagcgtt aaatgtctat ccgggccgcg
    22081 tgatggcgct ggtgggcgaa aacggcgcgg gtaaatccac catgatgaaa gtgcttactg
    22141 gtatctatgc tcgcgatgcc ggcacgcttt tatggctggg gaaagaaacg acatttaccg
    22201 ggccgaaatc ttcccaggaa gccgggattg ggattatcca tcaggaactg aacctgatcc
    22261 cgcagttgac cattgccgaa aacattttcc tcggtcgtga gtttgttaat cgctttggca
    22321 aaattgactg gaaaaccatg tatgccgaag cggataaatt gctggctaaa cttaacctgc
    22381 gctttaaaag cgacaagctg gtgggcgatc tttccatcgg tgaccagcaa atggttgaaa
    22441 tcgccaaagt gctgagcttt gagtcgaaag tcatcattat ggatgaaccg accgatgcgc
    22501 tgaccgatac cgaaaccgaa tccctgttcc gcgtcatccg cgaactaaaa tcgcaaggcc
    22561 gcggtattgt ctatatctcc caccgcatga aagaaatctt cgagatttgc gatgacgtta
    22621 ccgtttttcg tgacgggcaa tttattgctg agcgcgaagt ggcatcactg accgaagatt
    22681 cgctgattga gatgatggtg ggtcgcaagc tggaagatca atatccgcac ctggacaaag
    22741 cgccgggaga tatccgcctg aaagtcgata atctctgcgg acttggcgtt aacgatgtct
    22801 cttttacttt acgcaaaggc gaaattcttg gcgtctctgg tttgatgggc gcaggtcgta
    22861 ccgaactgat gaaagtgctc tacggcgcac taccgcgcac cagcggttac gtcaccctgg
    22921 atgggcatga agtcgttacc cgttcaccgc aggatggcct ggcaaacggc attgtgtata
    22981 tctccgaaga ccgtaaacgt gacggtttag tgttgggcat gtcagtaaaa gagaacatgt
    23041 cgctgacagc gctgcgctac ttcagccgcg ctggcggcag tttgaagcat gccgatgaac
    23101 agcaggctgt gagtgatttc attcgtctgt ttaatgtgaa aacaccgtcg atggaacagg
    23161 caattggtct gctttccggt ggcaatcagc aaaaagtggc gattgcccgc ggtctgatga
    23221 cacgccccaa agtgttgatc cttgatgaac ctacccgtgg cgtagatgtc ggcgcgaaaa
    23281 aagagatcta tcaactgatt aaccagttca aagccgatgg cttgagcatc attctggtgt
    23341 catcggagat gccagaagta ttaggcatga gcgatcgcat catcgtcatg catgaagggc
    23401 atctcagcgg ggaatttact cgtgagcagg ccacccagga agtgttaatg gctgccgctg
    23461 tgggcaagct taatcgcgtg aatcaggagt aaaaaaatga caacccagac tgtctctggt
    23521 cgccgttatt tcacgaaagc gtggctgatg gagcagaaat cgcttatcgc tctgctggtg
    23581 ctgatcgcga ttgtctcgac gttaagcccg aactttttca ccatcaataa cttattcaat
    23641 attcttcagc aaacctcggt gaacgccatt atggcggtcg ggatgacgct ggtgatcctg
    23701 acgtcgggta tcgacttgtc ggtaggttct ctgttggcgc tgaccggcgc agttgctgca
    23761 tctatcgtcg gcattgaagt caatgcgctg gtggctgtcg ctgccgctct cgcgttaggt
    23821 gccgcaattg gtgcggtaac cggggtgatt gtagcgaaag gtcgcgtcca ggcgtttatc
    23881 gctacgctgg ttatgatgct tttactgcgc ggcgtgacca tggtttatac caacggtagc
    23941 ccagtgaata ccggctttac tgagaacgcc gatctgtttg gctggtttgg tattggtcgt
    24001 ccgctgggcg taccgacgcc agtctggatc atggggattg tcttcctcgc ggcctggtac
    24061 atgctgcatc acacgcgtct ggggcgttac atctacgcgc tgggcggcaa cgaagcggca
    24121 acgcgtcttt ctggtatcaa cgtcaataaa atcaaaatca tcgtctattc tctttgtggt
    24181 ctgctggcat cgctggccgg gatcattgaa gtggcgcgtc tctcctccgc acaacccacg
    24241 gcggggactg gctatgagct ggatgctatt gctgcggtgg ttctgggcgg tacgagtctg
    24301 gcgggcggaa aaggtcgcat tgttgggacg ttgatcggcg cattaattct tggcttcctt
    24361 aataatggat tgaatttgtt aggtgtttcc tcctattacc agatgatcgt caaagcggtg
    24421 gtgattttgc tggcggtgct ggtagacaac aaaaagcagt aataacgact acaggacatc
    24481 ttgaatatga acatgaaaaa actggctacc ctggtttccg ctgttgcgct aagcgccacc
    24541 gtcagtgcga atgcgatggc aaaagacacc atcgcgctgg tggtctccac gcttaacaac
    24601 ccgttctttg tatcgctgaa agatggcgcg cagaaagagg cggataagct tggctataac
    24661 ctggtggtgc tggactccca gaacaacccg gcgaaagagc tggcgaacgt gcaggactta
    24721 accgttcgcg gcaccaaaat tctgctgatt aacccgaccg actccgacgc agtaggtaat
    24781 gctgtgaaga tggctaacca ggcgaacatc ccggttatca ctcttgaccg ccaggcaacg
    24841 aaaggtgaag tggtgagcca cattgcttct gataacgtac tgggtggcaa aatcgccggt
    24901 gattacatcg cgaagaaagc gggtgaaggt gccaaagtta tcgagctgca aggcattgct
    24961 ggtacatccg cagcccgtga acgtggcgaa ggcttccagc aggccgttgc tgctcacaag
    25021 tttaatgttc ttgccagcca gccagcagat tttgatcgca ctaaaggttt gaacgtaatg
    25081 cagaacctgt tgaccgctca tccggatgtt caggctgtat tcgcgcagaa tgatgaaatg
    25141 gcgctgggcg cgctgcgcgc actgcaaact gccggtaaat cggatgtgat ggtcgtcgga
    25201 tttgacggta ctccggatgg cgaaaaagcg gtgaatgatg gcaaactagc agcgactatc
    25261 gctcagctac ccgatcagat tggcgcgaaa ggcgtcgaaa ccgcagataa agtgctgaaa
    25321 ggcgagaaag ttcaggctaa gtatccggtt gatctgaaac tggttgttaa gcagtagttt
    25381 taatcaggtt gtatgacctg atggtgacat aaatacgtca tcgacagatg aacgtgtaat
    25441 ataaagaaaa gcagggcacg cgccacccta acacggtggc gcattttatg gacatcccga
    25501 atatgcaaaa cgcaggcagc ctcgttgttc ttggcagcat taatgctgac cacattctta
    25561 atcttcaatc ttttcctact ccaggcgaaa ccgtaaccgg taaccactat caggttgcat
    25621 ttggcggcaa aggcgcgaat caggctgtgg ctgctgggcg tagcggtgcg aatatcgcgt
    25681 ttattgcctg tacgggtgat gacagcattg gtgagagcgt tcgccagcag ctcgccactg
    25741 ataacattga tattactccg gtcagcgtga tcaaaggcga atcaacaggt gtggcgctga
    25801 tttttgttaa tggcgaaggt gagaatgtca tcggtattca tgccggcgct aatgctgccc
    25861 tttccccggc gctggtggaa gcgcaacgtg agcgtattgc caacgcgtca gcattattaa
    25921 tgcagctgga atcaccactc gaaagtgtga tggcagcggc gaaaatcgcc catcaaaata
    25981 agactatcgt tgcgcttaac ccggctccgg ctcgcgaact tcctgacgaa ctgctggcgc
    26041 tggtggacat tattacgcca aacgaaacgg aagcagaaaa gctcaccggt attcgtgttg
    26101 aaaatgatga agatgcagcg aaggcggcgc aggtactgca tgaaaaaggt atccgtactg
    26161 tactgattac tttaggaagt cgtggtgtat gggctagcgt gaatggtgaa ggtcagcgcg
    26221 ttcctggatt ccgggtgcag gctgtcgata ccattgctgc cggagatacc tttaacggcg
    26281 cgttaatcac ggcgttgctg gaagaaaaac cattgccaga ggcgattcgg tttgcccatg
    26341 ctgccgctgc gattgccgta acacgtaaag gcgcacaacc ttccgtaccg tggcgtgaag
    26401 agatcgacgc atttttagac aggcagaggt gacgcttggc tacaatgaaa gatgttgccc
    26461 gcctggcggg cgtttctacc tcaacagttt ctcacgttat caataaagat cgcttcgtca
    26521 gtgaagcgat taccgccaaa gttgaagcgg cgattaaaga actcaattac gcgccatcag
    26581 ccctggcgcg tagcctcaaa ctcaatcaaa cacataccat tggcatgttg atcactgcca
    26641 gtaccaatcc tttctattca gaactggtgc gtggcgttga acgcagctgc ttcgaacgcg
    26701 gttatagtct cgtcctttgc aataccgaag gcgatgaaca gcggatgaat cgcaatctgg
    26761 aaacgctgat gcaaaaacgc gttgatggct tgctgttact gtgcaccgaa acgcatcaac
    26821 cttcgcgtga aatcatgcaa cgttatccga cagtgcctac tgtgatgatg gactgggctc
    26881 cgttcgatgg cgacagcgat cttattcagg ataactcgtt gctgggcgga gacttagcaa
    26941 cgcaatatct gatcgataaa ggtcataccc gtatcgcctg tattaccggt ccgctggata
    27001 aaactccggc gcgcctgcgg ttggaaggtt atcgggcggc gatgaaacgt gcgggtctca
    27061 acattcctga tggctatgaa gtcactggtg attttgaatt taacggcggg tttgacgcca
    27121 tgcgccaact gttatcacat ccgctgcgtc ctcaggccgt ctttaccgga aatgacgcta
    27181 tggctgttgg cgtttaccag gcgttatatc aggcagagtt acaggttccg caggatatcg
    27241 cggtgattgg ctatgacgat atcgaactgg caagctttat gacgccacca ttaaccacta
    27301 tccaccaacc gaaagatgaa ctgggggagc tggcgattga tgtactcatc catcggataa
    27361 cccagccgac ccttcagcaa caacgattac aacttactcc gattctgatg gaacgcggtt
    27421 cggcttagat ttacgctgtc ttttgatcaa attattacca tcggttgttt tcagaagcat
    27481 gaacattgct gctgaagcaa cagtaataat gcccatcgtg ataaacgtat agtggaattg
    27541 ttcgacagtc gttgtgcctt ccatcccttc ataaacgcga aggacggccg cacttacagc
    27601 aacgcctaaa ctaatcgaca gttgctgcgt gaccgccaga acactgttac cgctgctggc
    27661 gttgtcatcg gtcagatcgg caagtgtgat ggtattcatc gcggtaaatt gcgtcgacat
    27721 agccatccct aatataaaca acggcaagat cagcatccat atcgccattg ccggtgattg
    27781 caaagagaac tgagcgatca ttagcccaat aatcaccgtg atccccacta acgtatggcg
    27841 atagcccaga cgacgtaaga cttgggtaac catcgatttt gcaataatgg aacctaacgc
    27901 tgtcggtgcc atcatacagc cggcaataaa cgcctgataa ccaaatccta cctgtaacat
    27961 caatggcata aggaacggta cacaaccggt ccccagacgg gttgcaatat tgcctacgat
    28021 accgattgag aaagtgcggg ttttaaataa atctaatgaa attaatgggt ttggcgtgtg
    28081 tcgcgcatgg agaatataga gaagcagtaa cccgatgctg gtgacaatta ccgtcaaggc
    28141 aatccagctg gcgacaatct tttccccgaa tagctctatt ccgcttgaga agagaacaag
    28201 gctgaggcca aacagcaaaa agccagtgat atcgaatctg cgtcgtgcgg tggtgaaatt
    28261 gggcatatgt ttgcgcgcgt aaagaaggcc cgcaatacct atggggatat tgattaaaaa
    28321 tatccagtgc caggttgccc aggtgaccag cacgccgcca agaacggggc ctaaaattgg
    28381 ccccaccaga cccggcatgg cgacaaaatt caatactgga agaagttcat tacgaggata
    28441 agcgcgcagt aaggccagcc gagcaacagg catcatcatt gcgccgccta tcccctgaat
    28501 aacccggaag acaaccagct gtggtagcga attagaaagt gcgcaggcca gagaacccaa
    28561 tgtgaacaga ctcacggcaa gggtaaaaat gcgacgcgta ccgaagcgat cggctagcca
    28621 tccgcttacc ggaataagca tcgccaccgt cagcgtataa ctgatgatgg ctgattgcat
    28681 cgcgagagga gaacgattaa ggctatgagc gattgcgggt aaggcggtat tcagaatagt
    28741 ggcatcaagt gcctgcatga agaaggccat cgccgcgatc cacggcaaac ccgccatact
    28801 gcgcttcttt ttatcgctca ttcaatgtcc tgttatcggg ttgtcactta tcaggtgagc
    28861 gtagcagcgc ctgacaagct ttaaatgccg cgtcgccatc gctttggaca atcgcatcga
    28921 caatcgcctg gtgcagatcc agctttatca ctgtgtcgct ggtaattgac gtgaagtaag
    28981 tgtgataaac cgaatggaat agcgaggcga atgaggtcaa aaacggattg gcgctcattt
    29041 catagatatg ctcatgccag gccatatcga cttcgatcca gcgttcacgg cgaaagttct
    29101 cttttaatgc cgccatttcg gccattaacg tattgagatg cgccttctgt tccgcggtgc
    29161 caaccgttgc agccagtagg caagcttgcg gctccagaca gatacgcata accagaaagt
    29221 gatcgatgac ctgatgaaag ttctcttctg tcatccacca ggtaagcaat tcctgatcaa
    29281 gaaaattcca gtttgattgt ggcatgaccc gggtaccaat tcgcggtcgc ggtaaaacca
    29341 tcccttttgc cgttaacgtt ttgaccgctt cgcgtaccgc tgtacgactc actccaaatt
    29401 gctcgcccag ctcaatctca ccaggcaaaa tggtgccggg ttcatattca ccttttaaga
    29461 tgcgttgcgc cagcttctca gccagaacat acgaaaggtt tttctgtgca gctaactgtt
    29521 gtgcgcttaa aggcattact aatcttcctt tttcttttta ttcctcctta gtatgccacc
    29581 aggaagtgtg attacggttg caaaaacggc aaattgcttg ttttatggca cattaacggg
    29641 gggggggttg ct
//
LOCUS       DAZKIV010000043        29514 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZKIV010000043 DAZKIV010000000
VERSION     DAZKIV010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.043"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..356)
                     /gene="yajD"
                     /locus_tag="ACHOT6_001909"
     CDS             complement(9..356)
                     /gene="yajD"
                     /locus_tag="ACHOT6_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFP9947136.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(484..1455)
                     /gene="secF"
                     /locus_tag="ACHOT6_001910"
     CDS             complement(484..1455)
                     /gene="secF"
                     /locus_tag="ACHOT6_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFP9947137.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(1466..3313)
                     /gene="secD"
                     /locus_tag="ACHOT6_001911"
     CDS             complement(1466..3313)
                     /gene="secD"
                     /locus_tag="ACHOT6_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFP9947138.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(3341..3673)
                     /gene="yajC"
                     /locus_tag="ACHOT6_001912"
     CDS             complement(3341..3673)
                     /gene="yajC"
                     /locus_tag="ACHOT6_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFP9947139.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(3696..4823)
                     /gene="tgt"
                     /locus_tag="ACHOT6_001913"
     CDS             complement(3696..4823)
                     /gene="tgt"
                     /locus_tag="ACHOT6_001913"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFP9947140.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(4879..5949)
                     /gene="queA"
                     /locus_tag="ACHOT6_001914"
     CDS             complement(4879..5949)
                     /gene="queA"
                     /locus_tag="ACHOT6_001914"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFP9947141.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            6042..6623
                     /gene="acpH"
                     /locus_tag="ACHOT6_001915"
     CDS             6042..6623
                     /gene="acpH"
                     /locus_tag="ACHOT6_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFP9947142.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(6628..8445)
                     /gene="malZ"
                     /locus_tag="ACHOT6_001916"
     CDS             complement(6628..8445)
                     /gene="malZ"
                     /locus_tag="ACHOT6_001916"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFP9947143.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(8601..9974)
                     /gene="proY"
                     /locus_tag="ACHOT6_001917"
     CDS             complement(8601..9974)
                     /gene="proY"
                     /locus_tag="ACHOT6_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFP9947144.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(10050..11369)
                     /gene="brnQ"
                     /locus_tag="ACHOT6_001918"
     CDS             complement(10050..11369)
                     /gene="brnQ"
                     /locus_tag="ACHOT6_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFP9947145.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(11776..13071)
                     /gene="phoR"
                     /locus_tag="ACHOT6_001919"
     CDS             complement(11776..13071)
                     /gene="phoR"
                     /locus_tag="ACHOT6_001919"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFP9947146.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(13129..13818)
                     /gene="phoB"
                     /locus_tag="ACHOT6_001920"
     CDS             complement(13129..13818)
                     /gene="phoB"
                     /locus_tag="ACHOT6_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFP9947147.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            14008..15210
                     /gene="sbcD"
                     /locus_tag="ACHOT6_001921"
     CDS             14008..15210
                     /gene="sbcD"
                     /locus_tag="ACHOT6_001921"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFP9947148.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQYLFTTTLH
                     TLAGEHEA"
     gene            15207..18350
                     /gene="sbcC"
                     /locus_tag="ACHOT6_001922"
     CDS             15207..18350
                     /gene="sbcC"
                     /locus_tag="ACHOT6_001922"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFP9947149.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     ANQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNTLAAITLTLTADEVASALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGDIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            18476..19660
                     /gene="araJ"
                     /locus_tag="ACHOT6_001923"
     CDS             18476..19660
                     /gene="araJ"
                     /locus_tag="ACHOT6_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFP9947150.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(19803..20711)
                     /gene="mak"
                     /locus_tag="ACHOT6_001924"
     CDS             complement(19803..20711)
                     /gene="mak"
                     /locus_tag="ACHOT6_001924"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFP9947151.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTRVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            20836..21747
                     /gene="rdgC"
                     /locus_tag="ACHOT6_001925"
     CDS             20836..21747
                     /gene="rdgC"
                     /locus_tag="ACHOT6_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFP9947152.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(21825..21908)
                     /locus_tag="ACHOT6_001926"
     CDS             complement(21825..21908)
                     /locus_tag="ACHOT6_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947153.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(21905..22246)
                     /locus_tag="ACHOT6_001927"
                     /pseudo
     CDS             complement(21905..22246)
                     /locus_tag="ACHOT6_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(22394..22678)
                     /gene="ppnP"
                     /locus_tag="ACHOT6_001928"
     CDS             complement(22394..22678)
                     /gene="ppnP"
                     /locus_tag="ACHOT6_001928"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFP9947154.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(22750..23427)
                     /gene="aroM"
                     /locus_tag="ACHOT6_001929"
     CDS             complement(22750..23427)
                     /gene="aroM"
                     /locus_tag="ACHOT6_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFP9947155.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(23685..23876)
                     /gene="yaiA"
                     /locus_tag="ACHOT6_001930"
     CDS             complement(23685..23876)
                     /gene="yaiA"
                     /locus_tag="ACHOT6_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFP9947156.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTVQEAMDAKKRYEDPDKE"
     gene            complement(23926..24450)
                     /gene="aroL"
                     /locus_tag="ACHOT6_001931"
     CDS             complement(23926..24450)
                     /gene="aroL"
                     /locus_tag="ACHOT6_001931"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFP9947157.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQFNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(24633..25091)
                     /gene="yaiI"
                     /locus_tag="ACHOT6_001932"
     CDS             complement(24633..25091)
                     /gene="yaiI"
                     /locus_tag="ACHOT6_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFP9947158.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQNGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            25211..26020
                     /gene="proC"
                     /locus_tag="ACHOT6_001933"
     CDS             25211..26020
                     /gene="proC"
                     /locus_tag="ACHOT6_001933"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFP9947159.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(26037..27152)
                     /gene="adrA"
                     /locus_tag="ACHOT6_001934"
                     /gene_synonym="dgcC"
     CDS             complement(26037..27152)
                     /gene="adrA"
                     /locus_tag="ACHOT6_001934"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFP9947160.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(27254..27574)
                     /gene="psiF"
                     /locus_tag="ACHOT6_001935"
     CDS             complement(27254..27574)
                     /gene="psiF"
                     /locus_tag="ACHOT6_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFP9947161.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(27693..29108)
                     /gene="phoA"
                     /locus_tag="ACHOT6_001936"
     CDS             complement(27693..29108)
                     /gene="phoA"
                     /locus_tag="ACHOT6_001936"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFP9947162.1"
                     /translation="MKQNTIALALLSLLFTPVTKARTPEMPVLENRAAQGDITASGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(29209..29469)
                     /gene="iraP"
                     /locus_tag="ACHOT6_001937"
     CDS             complement(29209..29469)
                     /gene="iraP"
                     /locus_tag="ACHOT6_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFP9947163.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         7337 a         7979 c         7327 g         6871 t
ORIGIN      
        1 cgttttaatc acttcttctt gttcatcatc gctttcaggt cagcgaatgg gttgtacttc
       61 gcttcaccga catctttctg cgcatcttcc cctgcgataa cggtcgtacc atactgatcc
      121 gcttcggtat atttcgaatg ctcatgatcg tggcaataga gacaaagcaa ttcccagtta
      181 ctgccatctt ccgggttatt ggtatggtcg tgatcaatgt ggtgaacagt aagttcacgc
      241 aggttggaat aaacaaactc gcgggaacag cgaccgcaga cccacggata gatttttaat
      301 gctttttcgc gatagccgct ttctaaccgc gcatagtttt ttgggatgat agccatgaag
      361 agttacctgg atgaaatgta gggttacgcg cagtttccca agatgcgggg attttcgcaa
      421 tctccataca tcaaaaaaat cccgatcttc tgaccgggat tttcaacatc aacgggaact
      481 tgattacggc agaattgacg gctgatccgc cccttctttt tccactttct gctgcaacat
      541 gtgttcgcgc ttcataccca gtttcagagc caacgcagat gccacataga tggaagatgc
      601 agtaccgatg gaaacaccga taagcatggt cagcgagaag ccttccagta ccggaccacc
      661 gaagaggtac agcatcagga taaccatcaa ggtagtaccg gatgtgatca aggtacggtg
      721 cagcgtctgg gtcaaggaca cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg
      781 gaagttttca cgaatacggt ccgataccac gatactgtcg ttaagcgagt aaccgataac
      841 cgacatcaac gatgccacaa tggtcaggtc aatctcgata tggaataacg acaaaatacc
      901 cagcgtaatg ataacgtcgt gcgccagcgc aataacaacc cctgccgcca gtcgccactc
      961 aaagcgaaaa cctacgtaca caaggataga cagcagcgcc gccatcaacg ccatcgcacc
     1021 ggtttgcgca aggtctgccc ccacgctcgg accgacgaac tcaatacgct tcactgctgc
     1081 attctgattg gtggattcgt taatcacctt cagaacctgg ctgcccagca cctgaccgcc
     1141 ggtttcgcct tcagcaggcg gcatacggac catgatgtcg tggctgctac caaagttttg
     1201 cagcattggc tcttcaaaac cggctttttg caatgcatca cgcattacgt caatttcagc
     1261 cggtttttcg agcgtaattt cgataaccgt accaccggtg aaatccagcc cccagttaaa
     1321 gccgcgcacg cccataataa cgatagcggc gattaacagc agaccagaga tgccgaaagc
     1381 ccagtagtcc cagcgcataa agtcatagac tttacggccg tggtttagtt gttcaacagt
     1441 atattcctgt gccacatcgc actcctcaga ttgacagctt cttgacacgc ttgccgccat
     1501 atagcaggtt tacgatggca cgcgtaccga caatcgcggt aaacatcgac gtcgccacac
     1561 cgataccggt agtaatcgcg aaccctttaa ttgccccggt acccactgcg tacaggatga
     1621 tgactttaat cagcgtggtg atgttcgcat cgaagataga actgaatgcg ccacgataac
     1681 cttcatcaat tgcctgttga acagtacgtc cgttgctcaa ctcttcttta atacgttcgt
     1741 tgatcagtac gttcgcatcg accgccaccg caagggttaa gacgataccc gcgatacctg
     1801 gcatactcag cgttgcgcct ggcaacagcg acataatgcc gacgattaag atcaagttgg
     1861 caatcagagc actggtcgca atcagaccaa acttcttata gaagatgatc atgaacagaa
     1921 tagaaaccag cagaccggca aggcaagcct ccagcccctg ttcaatgttc tgcataccca
     1981 gggttgggcc aatggtacgt tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac
     2041 gcagcagcag tgacagctga cgggcttcgt tcgggttgtt gatgccggtg atacggaagc
     2101 tgttacccag acgagactgg atgttggcga tgttaatcac ctcttcctgt ttcaccagaa
     2161 ccgcacgacc atttgcatct ttcttaccgc tgtctttgta ctccacaaac agggttgcca
     2221 tcggtttgcc gatgttgtcc ttagtgaagt tagacatgat gttaccacca gcgctatcga
     2281 gcgagatgtt aacctgcggt tggttgtatt cgtcctggct ggaagtggag tcggtgatat
     2341 ggtcaccggt cagaattacg cgtttgtaca gcacaactgg ctgaccttcg cgggtctgtt
     2401 tcacttcaga gtcgcccggt acgcgaccgg atgccgcagc ggcctggtca acgttggtgt
     2461 ttaccagacg gaattccagc gttgccgtcg cacccagaat ctctttcgca cgcgcagtgt
     2521 cctgaatacc tggcagttca acaacgatac ggtcagcacc ctgacgctga accaccggct
     2581 ccgccacgcc aagttggttt acacggttac gcaggatatt gatgttctgc tgcaccgcat
     2641 attcacgcgc ttcactcaga cgagcatcgc tcattaccgc acgcagctgg ttgctgccct
     2701 ggctactaat caccaggtcc ggatggcgct tgctcagata cgcaatggct tcatcacgag
     2761 ctttagcatc gcggaaagta atgctcaggc cgtagttgtt ttctttacga acggtggtat
     2821 acgggatgcc cttttcacgc agatcgctgc gcaggctatc gatattttgt tcctggagtt
     2881 tgccaagcgc ggtgtccata tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa
     2941 ggccgagctt catcggctca gcgtgaatag ctgccagcca gcgcggcgtt gccggggcaa
     3001 ggttaagcgc cacgacgtat ttgtcaccca taacgcccat taatgcttca cgagcgcgca
     3061 gctgggtgtc agtggagtcg aagcgcgcaa gaatagcgcc ctcttccagt gccacagact
     3121 tagcagttat tttttcttct tgtaacgttt tctggacctg gatcagcgtt tgctcactgg
     3181 cggcgactcc gcgcgcacca gtgatctgaa cagccggatc ctcaccaaac aggttgggaa
     3241 gcgcatacag cagaccgatg acaatcacca cgatcagcat gacgtacttc cacaaaggat
     3301 aacggtttaa cacggcaatt cccttaggga aaaattttaa ttacagcgcc ttcatggtgc
     3361 ctttcggcag gacggcagct acgaagtcac gtttaataac tacttcagtg gtgtcattca
     3421 gcgcgatagc aatgtagccg ttttccgcta ctttggttac gcgaccaacc aggccaccgt
     3481 tcgtcagaac ttcatcacct ttggcaatgg agtccatcag ctttttgtgt tctttggtgc
     3541 gcttctgctg tggacgcagg atcatgaaat agaaaatcag accgaatacc accagcatca
     3601 aaatcaaaga catcgggcta ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa
     3661 taaaaaagct cattaaattt ccctcattat taatattaat caacgttcaa aggtggtact
     3721 tctcgcccct gacgctggta aaaatcagtt acgaagctct ctaatttacc ctcttcaata
     3781 gccttgcgta aacccgccat caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt
     3841 cgcgcgccta atatttcgtt gcaacggtca agatgatgca agtaagcgcg tgaataattg
     3901 cgacaggtgt agcaatcaca ctcaggatcg agtgggccag tatcgctctt atacttcgca
     3961 ttgcggattt tcaccacgcc atcggtcacg aacaaatgac catttcgggc gttgcgggtt
     4021 ggcattacgc agtcaaacat atcgatacca cgacgtacgc cttcaaccag gtcttctggt
     4081 ttaccaacgc ccatcaggta acgcggtttg tctgccggaa tttgcgggca tacatgctcc
     4141 agaatgcggt gcatatctgc tttcggctca cccacagcca gaccgccgac agcgtagcca
     4201 tcaaaaccga tatctaccag acctttaaca gaaatatcac gtaaatcttc gtaaacgctg
     4261 ccctgaatga taccaaacag cgcatttttg tttccgagac tgtcaaaacg ctcacggcta
     4321 cgcttcgccc aacgcagaga catctccatg gagcgttttg cgtaatccca gtcagcagga
     4381 tacggcgtac actcatcaaa gatcatgacg atatccgaac caagatcgta ctgaatctcc
     4441 attgattttt caggatcgag gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg
     4501 ccctgttcgg tgattttacg aatatcgcca aggctgaaga cctggaagcc gccggagtcg
     4561 gtgaggatcg gccccttcca ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt
     4621 tcctggcccg ggcgcagcca caggtggaag gtgttgccga ggataatttg cgcgccagtg
     4681 gcttcaactt cttccggcgt catccctttt acggtgccgt aggtgccaac aggcataaaa
     4741 caaggcgttt ccactacgcc acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg
     4801 gtggtgtcca gttcaaattt cattttttct ccactacgtc agaaaaacag tccaacgttt
     4861 taaaccagcg ccgcggaatt actccccgac gcgctcatta attgcctgcg gattgtacgt
     4921 gataaacatc gcatcaccgt aactaaaaaa gcgatatttc tcttctaccg ctgctttata
     4981 ggcgttcatg gtgtgttgat aaccggcaaa ggccgaaacc agcataatca gcgtcgactc
     5041 tggcaagtgg aagttcgtca ccagcgcatc gaccactttg tactggaagc ccggatagat
     5101 aaagatttgg gtatcgtcga agaacggttc aatgagatcg tttttcgctg cctgagccgc
     5161 gctttccagc gaacgtactg aagtggtgcc aaccgcaatc acccggttac cgcgcgcttt
     5221 cgccgccagt accgcgtcta ccacatcctg cggtacttca gcgtattccg agtgcatgat
     5281 gtgatcttca atggtgtcga cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt
     5341 cacaaacgcc atctccacgc ctttggcgcg caatttttcc agcaaaggct cgtcaaaatg
     5401 cagaccagcg gtcggggctg caaccgcgcc cggtttttcg ctataaacgg tttgataaag
     5461 ttcgcggtca gcgtcttcgt ccggacggtc gatatacggc ggcagcggca tatggccgat
     5521 actgttgagg atatccagta ccgaacgatc atcattgaat tcgacttcaa acagtgcgcc
     5581 gtggcgcgcg gtcattgttg cgttaatact ttcgtcatcg cccagcagca gttctgcgcc
     5641 aggttttggc gctttcgagg cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg
     5701 ctcaaccagc acttcaatct tgccgccgct ggctttacgc ccaaacaggc gcgccgggat
     5761 cacgcgggta ttattaaaaa ccagaagatc gccggggttg agcttatcaa gtaaatcggt
     5821 gaaagtaccg tgcgtcagcg cgcccgtcgg cccgtccagc gacagtaaac gacagctact
     5881 gcgttcaggc atgggatagt gggcaatcag ggattcgggc aattcaaagg agaaatcggt
     5941 aacgcgcatg acactgactc agactaaaat aagaggcggg tagtctagtg ccggggcgct
     6001 ctccctgcaa cctttacccc cttttggata gaatataatc gatgaatttt ttagctcacc
     6061 tgcatttagc ccatctcgcg gaaagctcgc tttccggcaa tttactggct gatttcgtac
     6121 gcggaaatcc tgaagaaagt tttccgcccg acgtcgtggc tggcattcat atgcatcgac
     6181 gtatcgacgt attaaccgac aatctgccgg aagtccgcga agcacgggaa tggtttcgta
     6241 gtgaaactcg ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc
     6301 gccactggtc gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg
     6361 agcaagtgat gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact
     6421 tgtggtcaga gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa
     6481 acggcatggc aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag
     6541 acgctcatta tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc
     6601 aggcgtcaca caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg
     6661 caaagccaga atcccgtttg tcagttgccc atggccttct ttgcgttgcc attgcgcgac
     6721 attaagaaac ggtgacgcgg ggagcaccac ttcacaggcc tcgccacggt tgattgccac
     6781 cagtacacgt tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag
     6841 cacctgacag ccgccacgac gcagcgcctg acttttctta cgcagcgcaa tcattcgctg
     6901 gtacagcgcg aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca
     6961 aaacggatcg tttttgccat ccagtcctac ttcatcaccg taataaatgc acggtacacc
     7021 aggccaggtg aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag
     7081 cgttttaaat cgcgcagtat cgtggctgtc gagctgatta aacatacgta attgttgttg
     7141 atgagaaagc cctgcgcggt aattatccat ccaggccata caggtttggg catcaatttg
     7201 ctgcggatcg taagagatat cggtattggc aagaaatccc cacaacggga atgtgaagcc
     7261 acgatagttc atggcggcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa
     7321 atgttcgccg acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccagc
     7381 aacgtgctgc atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag
     7441 ccgccagccg tccatactcc acggcgcttt cagccaatgg cggacaatac tgtcttcccc
     7501 gcgataaatt tcattcacca gactttccga ctgataatcc agcttcggca agctggcata
     7561 gccaagccag tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga
     7621 ttcagggttg tgacaagctc cccccgtgcc acgattgtgc ctgtcaaacc aggcatggga
     7681 atcgccactg tggttaaata cgccgtccag caccagccgc attcccagct gctgcgtatt
     7741 gtgtcgcaaa cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc
     7801 ctcggtatcg tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgccgt
     7861 cacgccaagc tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata
     7921 gaacgttgat ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc
     7981 ttgtccggct gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg
     8041 cgcaaaacga tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat
     8101 atccggtaca tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg
     8161 cggtgtaaac caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg
     8221 ttgtccgctg gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact
     8281 gcgctgctta tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat
     8341 gcgctgcggt gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg
     8401 tttaacaaat gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg
     8461 aaaatctgat caccttgcca tagcctaatg caggatcact catcatctga aggatatttg
     8521 ggggaggagg acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac
     8581 cggcttacag aacgcaagta ttattggttt tcagccagct gacgatcgtg gcgacgctta
     8641 aacatccagc caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc
     8701 gtatccgggt gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg
     8761 gtcgttgcta ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa
     8821 cggcgacgga aggcaatttg cgacagcagg atcataatcc acacccacac cgtggcgaag
     8881 gttgccagcg aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc
     8941 gcaaacagca gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac
     9001 gttttgctga aaattttcgg cgcgctgccc tgctctgcca taccgtggag catacggcct
     9061 acgccaaata catcactgtt aattgccgac agcgaagcag tcagcacaac aaagttaaga
     9121 atgctggcgg caaaggtaat gcccatatgc tggaacgtca gcacgaacgg gctaccggca
     9181 gtgccaacct gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac
     9241 accagaatac gcatcggcac ggagttaatc gcacgcggta tcgatttctc aggatctttc
     9301 gcttcaccgg cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc
     9361 aacgacatta ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta
     9421 tgaataccgg tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg
     9481 acaatcatga tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg
     9541 aataccttca cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa
     9601 atccagtgcg gcaccgtcgg gaaccagaca cccatataga taccaaaagc ggtcacatcg
     9661 gcgatggcga caataaggat ttcaaagcag taggtccagc cggtaatgta acctgccagc
     9721 gggccgaggt tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac
     9781 atttccccca gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac
     9841 aacacgctcg gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca
     9901 attgctgaac ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagcttg
     9961 ttcttacttt ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag
    10021 ccccgtggtt aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct
    10081 gccgcgcgat cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc
    10141 agaccttgtt cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca
    10201 gatgctttga tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc
    10261 acgcgggacg aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac
    10321 ggcggataaa tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg
    10381 ttagaaacca ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt
    10441 acgtactggg caaagaattc tgcacaagca caggtcaggc caactgccgt taccaggcag
    10501 gcgatgaaga ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg
    10561 taagcatgca gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa
    10621 cccagacgga acagcgccag gtacagcaga gtcagaccaa cacccgccat caggccagcc
    10681 cagacggtat aacgggtcag taggcgcgct tcggtaacgc cacgagaacg cgccgcgtta
    10741 acaataacga taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttgacg
    10801 aagccgttag aaaacgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc
    10861 ggccagacaa tcgcggcaac agacaggatg accagcgcga taattttcag cggcgcaagg
    10921 aagttgccca cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg
    10981 aaatagacca ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc
    11041 cccacttcaa aagaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg
    11101 taacaaaccg ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca
    11161 acaccgccgc caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta
    11221 atgaggaagc cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga
    11281 gggaaaataa tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg
    11341 atatcgcgcg atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta
    11401 acgttaaatt tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag
    11461 cgggaaatac tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc
    11521 agtcagtatg acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag
    11581 ggatctaaaa accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt
    11641 tccgtgaaaa agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta
    11701 acgcccgtat tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa
    11761 gatgacaaag gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact
    11821 gaaacgtgtt ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac
    11881 cgcatgtttc acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt
    11941 atcaacgcga taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc
    12001 gttatcttca acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg
    12061 cgtgccttcc ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag
    12121 ctgatcttcg ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt
    12181 tttctgactc agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac
    12241 cttttcattg agcaattgcg tcggtgcggc ttcaattttc gacagcgtta gcaactgctt
    12301 caccagccct tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac
    12361 cgcgccttcc agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg
    12421 cgtacgtaac tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg
    12481 catttgcgtg acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg
    12541 aatttccaga tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt
    12601 tttcaaatat tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc
    12661 ttccggccag cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc
    12721 ctcttccgtg gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt
    12781 aatcagattg cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc
    12841 gtatagtagc ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca
    12901 cagccaccat gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga
    12961 tgccagcaaa aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca
    13021 gcaaagtagc agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac
    13081 tccagttaag aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt
    13141 tgaaaaacga tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc
    13201 cagtgcttta cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt
    13261 agttccccag acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat
    13321 aaaaaagtgc agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat
    13381 cactcggtga gatgtcgggt cgagacttaa tccctgcatc tcaatcacct cttccaccgc
    13441 cattggcgaa atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa
    13501 cggcttggtg atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc
    13561 ccctctggcg gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg
    13621 tttgatgaac tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg
    13681 ccagggttca ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa
    13741 gccattttgt tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac
    13801 cagaatacgt ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg
    13861 acgtcattat gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag
    13921 gttattgatt tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg
    13981 cccatgcttt ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc
    14041 ggccagaact tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg
    14101 ctggagacag cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat
    14161 accggctcgc cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag
    14221 caaactggct gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat
    14281 gaatcgcgcg atatcatggc gttcctcaat actaccgtgg tcgccagcgc cggacatgcg
    14341 ccgcaaatct tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt
    14401 ttacgtccgc gtgacattat taccagccag gcggggctta acggtattga aaaacagcag
    14461 catttactgg cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg
    14521 cgcggcgatc agcctctgcc catcatcgcc acgggacatt taacgaccgt gggggccagt
    14581 aaaagtgacg ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac
    14641 tttccaccag ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc
    14701 atggaacatg ttcgctattg cggctccccc attccactga gttttgatga atgcggtaag
    14761 agtaaatatg tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg
    14821 aacgtaccgg taacgcaacc catggcagtg ctgaaaggcg atctggcgtc gattaccgca
    14881 cagctggaac agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc
    14941 actactgatg agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg
    15001 cctgtcgaag tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc
    15061 caacagcgtg aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca
    15121 ctggaagaac tggatgaatc gcagcagcaa cgtctgcagt atcttttcac cacgacgttg
    15181 cataccctcg ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga
    15241 actcattaaa aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc
    15301 tgtttgctat taccggccca acaggtgcgg ggaaaaccac cctgctggac gccatttgtc
    15361 tggcgctgta tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca
    15421 tgacccgcga taccgccgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt
    15481 accgtgcatt ctggagccag aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc
    15541 cacgcgtaga gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata
    15601 agctggaact gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc
    15661 tgctttcgca ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat
    15721 tgctcgagga gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc
    15781 agcacaaatc ggcccgcaca gagctggaga agctgcaagc gcaggccagc ggcgtcgcgt
    15841 tgctcacgcc ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag
    15901 aaaaacagtt acttaccgcg cagcagcaag aacaacaatc actaaactgg ttaacgcgtc
    15961 tggacgaatt gcagcaagaa gccagccgcc gtcagcaggc cttgcaacag gccttagccg
    16021 aagaagaaaa agcgcaacct caactggcgg cgctcagtct ggcacaaccg gcacgaaatc
    16081 ttcgtccgca ctgggaacgc atcgcagaac acagcgcggc gctggcgcat actcgccagc
    16141 agattgaaga agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc
    16201 accacgcggc gaatcagtca gcagaattac agcagcagca acaaagcctg aatacctggt
    16261 tacaggaaca cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt
    16321 tctcccaaca aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg
    16381 ctgagcaaaa acttaatacg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg
    16441 ctagcgccct ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc
    16501 atgggcagat tgttccccaa caaaaacgtc tggcgcagtt acaggtcgct atccagaatg
    16561 tcacgcaaga acagacgcaa cgtaacgccg cacttaacga aatgcgccag cgttataaag
    16621 aaaagacgca gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa
    16681 cgctggaagc tcaacgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca
    16741 ccagccaccc ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat
    16801 tactggcgct ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc
    16861 aactggatgc attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac
    16921 aagatgagca agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct
    16981 tgcagccaca ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc
    17041 tgcggttact cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa
    17101 ttatccagta tcaacagcaa attgaacaac gccagcagca gctcttaacg gcattggcgg
    17161 gttatgcact gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc
    17221 aagaagcgca gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc
    17281 agcagctgac gccgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag
    17341 aaactgtggc gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc
    17401 agcagcagac gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccagg
    17461 cgcagtttga caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg
    17521 cgctaatgga tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc
    17581 agcgccgtca ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac
    17641 aacaccgacc tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag
    17701 cgcaaactca ccaaaagttg cgtgaaaaca ccacgagtca aggcgatatt cgccagcagc
    17761 tgaagcagga tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa
    17821 tgacgcagca ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg
    17881 ataaattccg caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc
    17941 agcaacttac ccggctgcac gggcgctatc tgttacagcg caaagccagc gaggcgctgg
    18001 aagtcgaggt tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg
    18061 gcggcgaaag tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc
    18121 ataaaacacg tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa
    18181 cgctggatac cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg
    18241 tgattagcca cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga
    18301 tcaacggcct gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca
    18361 ggataatgaa tacagagggg cgaattatct cttggccttg ctggtcgtta tcctgcaagc
    18421 tatcacttta ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa
    18481 aaaagtcatt ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat
    18541 tatgggcgtg ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca
    18601 tatgatctcg tattatgcac tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc
    18661 cagccgctac tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa
    18721 cgccatgttc acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg
    18781 ctttccgcat ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc
    18841 cggaaaagtc accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct
    18901 gggcattccg ctgggaacgt atttaagtca ggaatttagc tggcgttaca cctttttatt
    18961 gatcgctgtt tttaatattg cggtgatggc atcggtctat ttttgggtgc cagatattcg
    19021 cgacgaggcg aaaggaaagc tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg
    19081 gttaattttc gccgccacga tgtttggcaa cgcaggtgtg tttgcctggt tcagctacgt
    19141 aaagccatac atgatgttta tttccggttt ttcggaaacg gcgatgacct ttattatgat
    19201 gttagttggg ctagggatgg tgctgggaaa tatgctaagt ggcaggattt caggacgtta
    19261 ttcaccactg cgcattgcag cagtgactga ctttataatt gtactggcac tgctgatgct
    19321 ctttttctgc ggcggcatga aaacaacgtc gcttattttt gcttttattt gttgcgcggg
    19381 attatttgcc ctttcagcac ctctgcaaat attgttactg caaaatgcca aaggcggaga
    19441 gttattaggt gccgcaggtg ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata
    19501 ttgcggaggt atgatgctga cgctggggct ggcatataat tacgtggcgc tgcctgccgc
    19561 cctgctttcg tttgctgcga tgtcatcgtt gctgctgtat ggtcgctata agcgccagca
    19621 agcggcggat agtccggtgc tggcgaaacc actggggtag attactgtca cggtgcgcgt
    19681 tgcttattgc cggatgcggc gtgaacgcct tatccgccct acgcggttct ggcacatttt
    19741 gtaggcctga taagacgcgg caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt
    19801 ttttactctt gtggccataa ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc
    19861 gccttacgca ccggcgtttc acattcgccg ccgaagacaa attgtttaat caactgccca
    19921 accgtttgat ataaacggtc tacattgctc atcccgcccc ccaggacaat cacatccgga
    19981 tcgagaatat tcacgacatg tgccagcgat tttgccagcc gcagctcgta gcgacgcaat
    20041 gccagttccg ctaccggatc gctttcttca accaggcgga taatttcact gcctttcagc
    20101 gcatgtccgc tcaaacgacg ataatccatc gcgaatcccg tgcccgaaat aaaggtttca
    20161 atacaacctt gtttaccgca ataacaaggg acttcctcgc gataacgcag ttcgtcttcg
    20221 tccatccacg gtagcggatt gtgtccccac tcacctgccg tgccattgcc gccgatatgc
    20281 gcccgcccat tgaatgccac gcccgcgccg catcccgtgc cgataatcac ggcaaatacc
    20341 gtctgcgctc ccgctgccgc gccatctact gcttctgaaa ccgccagaca gttagcgtca
    20401 tttgccagcc gcacttcccg ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg
    20461 ttgagccagg ttgaattggc attcttcacc acacgggtgt aaggcgaaat tgagccagga
    20521 atgcccatac ctaccgttcc gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg
    20581 gcgatcgttt caatagtctg ccggtaatca tcacgcggcg tgggcagacg atggcggtac
    20641 aactgctccc ctgcatcgcc cagtgcaatc acttcagttt tggtgccgcc taaatcgata
    20701 cctatacgca cggtactctc cttatttttt tcaatatcaa tagcgtagag acggacaact
    20761 ggattggcaa tgcaaggccg ccgacaattc gttatcatgc ccgctaaatt taacgacaag
    20821 gccgtggaaa ttatcatgct gtggttcaaa aatttaatgg tttaccgtct tagccgcgag
    20881 atttcgctgc gtgcagaaga gatggaaaaa cagctagcct cgatggcatt taccccatgc
    20941 ggcagccagg acatggcgaa gatgggctgg gttcctccga tgggatcgca cagcgatgcg
    21001 ttaacgcacg ttgccaatgg tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc
    21061 ccgtctccgg tgattaaaca ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag
    21121 gcgcgtaagc tgaagaaaac cgaaaaagat tcgctgaaag acgaagtgct gcactctctg
    21181 ttgccgcgtg ctttcagccg ttttagccag acaatgatgt ggatcgacac ggttaacggt
    21241 ttgattatgg tggactgcgc cagtgccaaa aaagcggaag atacgctggc actgctgcgt
    21301 aaaagcctgg ggtcgttacc tgtggtacca ttgagcatgg aaaacccgat tgaactgacg
    21361 ctgaccgaat gggttcgctc cggtagcgcg gcacagggct tccagctgct tgatgaagcc
    21421 gagctgaaat cgttgctgga agatggcggc gtgatccgcg cgaagaaaca agatctgacc
    21481 agcgaagaga tcaccaatca catcgaagcc ggaaaagtgg tgactaaact ggcgctcgac
    21541 tggcagcagc gcattcagtt tgtgatgtgc gacgatggtt cgctcaagcg tctgaagttt
    21601 tgcgacgagc tgcgcgatca aaacgaagat atcgaccgtg aagatttcgc ccagcgtttt
    21661 gatgccgatt tcatcctgat gactggtgaa ctggcagcgt taattcaaaa cctgattgaa
    21721 ggattaggtg gcgaagcaca acgttaattg ctgattttcc tttaatgccg gatgcgacgc
    21781 ctgccgcgtc ttatccggcg tacgaagcca caccagggat ataattattc gctacggcgg
    21841 gcaataattt ttagcgcagc aatattatgc gttttatgct gtaacttgct ccatggacgt
    21901 tgtgtcattg tttttcctca agccagtgac gcgcttcact gcgaattttc cgcattgccc
    21961 cggttttcac taaatcccgt aacagcgaaa gatcgggttg ttcgacaaat aagagtaatg
    22021 atggatcctc ttttaaccac gtcgttttca catcggcagc gagctgtgga ttattcatag
    22081 ctagcgaccg atgccgagac tcggtcaatg tcgttaacaa cgacgccggg gtatgtgcgt
    22141 tgcgtaatgc cgtttggcgc aaatgatcat tgcgactttg cagcaatgca cgccaccatt
    22201 catcatctga tttatctttt atcgtagcca gttccgcaac aatatctgga gatcttcact
    22261 gatatttata tcaaccttga taaattatga atatatcgga aattgaaaaa atcgcagcaa
    22321 aggaaatatt attatgggtt aaagaaagga tatttcagaa aaatgccctc ccgttcaagg
    22381 ggaaggcgag gaattacaga tagcggcaca gataagaggt gggttcggca acttgcagat
    22441 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacacc tgccagtcgg
    22501 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    22561 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    22621 tgctggaaaa gccgattgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    22681 actggccctg tttggtagtt gaacatctac ttcactatag gggccagagg cgctgactgt
    22741 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagacagcaa
    22801 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    22861 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccggcat caatgatttt
    22921 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    22981 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    23041 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    23101 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    23161 ctgattatcg agcacttcaa ccacaccttg caggtctcgc tccactttac gacgcgaaac
    23221 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    23281 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    23341 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    23401 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    23461 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    23521 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggattg
    23581 ttattgttta atattctaat tattagaaaa acatgaatta tgaaaaatgt gacgcagatc
    23641 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggttactc tttgtcaggg
    23701 tcctcatagc gttttttcgc atccatcgct tcctgaaccg tcggatgttc actgatcaac
    23761 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    23821 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    23881 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    23941 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    24001 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    24061 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    24121 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    24181 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgg
    24241 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    24301 ttcgacgatc tccgcgaccg tcatattgaa ctgtgattgc aaccactgat cggtatcgac
    24361 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    24421 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    24481 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    24541 aaagaaaaaa ttccacttaa agtgaaaatc tcaatacatc ccttactata ccaataaata
    24601 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    24661 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    24721 ccgccgttct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    24781 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcggat taagcgccgc tgcgcctttc
    24841 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    24901 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    24961 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    25021 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    25081 caaattgtca taaagtcatc ctttgttggg taatcctcta ttgtgtcgcg cttttgcctt
    25141 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    25201 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    25261 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatatacccc
    25321 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    25381 gcaagaagtg gcgcaaatcg ccgacatcat ttttgccgcc gttaaacctg gcatcatgat
    25441 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    25501 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    25561 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    25621 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    25681 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagtggtt cttcgccagc
    25741 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    25801 cgcccaggcg tataaatttg ccgctcaggc agtaatgggt tccgcaaaaa tggtgctgga
    25861 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    25921 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    25981 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    26041 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    26101 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    26161 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    26221 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    26281 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    26341 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    26401 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    26461 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcgg
    26521 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    26581 acccagccaa acagcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    26641 ggcgcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    26701 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    26761 cacataatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    26821 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    26881 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    26941 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    27001 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    27061 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    27121 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    27181 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    27241 gcactatcag gtatcaggcg gctttcttga ggcaggcact cataaactta ttacgatcat
    27301 cacctttcag agattgttgc gttgcttgat tattacattc gcgcatcttt tgctgctgtg
    27361 gcgtcaaact tttttcacca ggcgcagact tgctgttctt caggcaatca ctcatgtagg
    27421 tcttacgagc atcccctttc aacgcctgcg ccgtcgcctg ctgattacag gaggtcatac
    27481 gctgttgttg tggggttaag gttctctcgg cagcgccgac ggtggttaaa aaaaccagac
    27541 caaaaagcaa ggtaaccagt aatgttattt tcatagcacc atccctcttc atgttttaac
    27601 catgagcgtt tgcgcccgtg atctgccatt aagtctggtt gctaacagca aaaaaaccac
    27661 ccggcagcga aaattcactg ccgggcgcgg ttttatttca gccccagagc ggctttcatg
    27721 gtgtagaaga gatcggtctg gtcggtcagt ccaacaacat tggcggcatg cgggccatac
    27781 gccgcaatac gcaactgact gccggtatgt tcttgtgaat cctcttcaga gttcccgtaa
    27841 ctcatcacca tcactgcgcc atctttagta tttagcgcct gagtgaggcc cggcgctttg
    27901 gtatccggcg caacaacctg gctggcgtgg gcgtgatcag cggtgactat gaccagcgtg
    27961 ttaccctcct ttttagcgaa ttccagcgcc cgttgtacgg cttcatcgag atcgaccgtc
    28021 tcgccaattt gcccacaagg attcgcagcg tgatcctgtt tatcgattga cgcaccttca
    28081 acttgcagga aaaatccttt ctcattttta ctcaacaatt caatggcttt gtcggtcatc
    28141 tgtgccaggg ttggtacact gtcattacgt tgcggattag gcgtacaggt gactgcgggc
    28201 ttatcgatat tgccgtggta cgttgctttc ggccctagcc agcgcactgg catattgccg
    28261 tcagcaaaca ggccaagcag gggtttttgc tgattcgctt ccgtcaccga attcagtgag
    28321 gcagcatcgt tcaccaactg ataaccacgc gcctgtgcct gttcacgcag cgtttttccc
    28381 tgccattcac cagcggttgc cgtttcagca aaggtttttg cgccgccgcc aagcgtaacg
    28441 tcggcacgag cgttaagcag ctgttcggta atcgatcctt ttccgccttt ttccagagcg
    28501 ttacccggac atttttcact ggtcgcgctc ggaccgtagc atttgcgcga agtcacatgt
    28561 gccaccagcg cagcgggcgt ggcatcctgc aactctgcgg tagaaacgtt accggtcgcc
    28621 agacctgcgg cttttgccat ttccagaatc gttgggtgat ctttttcgtg aatatcgacg
    28681 cccagcgcgc cgttataggt tttgacaccg gttgaccagg cggttgctga tgcagccgag
    28741 tcggtgacgt agtccggttt gccagttttt ttattcagcg catagtgagt gtattgcccg
    28801 gtaagcggta aggcatcgat acctttaaaa aagccgcccg caccttcggc ataattacgt
    28861 gcggctgtaa tttccgaatc ccccatccca tcgccaatca gcaaaataat attttttgca
    28921 ggtttatcgc taagagaatc acgcagagcg gcagtctgat cacccgttaa acggcgagca
    28981 ccgccggatg cagtaatatc gccctgagca gcccggtttt ccagaacagg catttctggt
    29041 gtccgggctt ttgtcacagg ggtaaacagt aacgataaga gtgccagtgc aatagtgttt
    29101 tgtttcactt tattttctcc atgtacaaat acattaaaaa ataaaaacaa agcgactata
    29161 agtctcggtc gtgacaactt tatgacagct gttgaaaaga ttaacttttt actgacgagg
    29221 atgcttcaat aacttcttta cgtaatcgcg cagcagctcc gtatcgtcgt caggaatgct
    29281 ggcatcgggc tttacctcgt acagcgcccc ctctacctga tcaatcaagc gctgttggtc
    29341 attttgcgcc atattgcgaa gcattgcagt gacgataatc tccaaagctt ctacctgcgc
    29401 acacagttct ttcgactctt cttctttttg ggcaagctta aataacaact cagcaatgag
    29461 atttttcatg tctgtatttc cttatccaaa gtatggagaa gttaacatta tgtt
//
LOCUS       DAZKIV010000044        28787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZKIV010000044 DAZKIV010000000
VERSION     DAZKIV010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.044"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..94
                     /locus_tag="ACHOT6_001938"
     CDS             <1..94
                     /locus_tag="ACHOT6_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenylacetyl-CoA ligase"
                     /protein_id="HFP9947164.1"
                     /translation="TDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            195..1145
                     /gene="paaX"
                     /locus_tag="ACHOT6_001939"
     CDS             195..1145
                     /gene="paaX"
                     /locus_tag="ACHOT6_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFP9947165.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEALCQFTR"
     gene            1127..1717
                     /gene="paaY"
                     /locus_tag="ACHOT6_001940"
     CDS             1127..1717
                     /gene="paaY"
                     /locus_tag="ACHOT6_001940"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFP9947166.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            1947..2807
                     /gene="pdxI"
                     /locus_tag="ACHOT6_001941"
     CDS             1947..2807
                     /gene="pdxI"
                     /locus_tag="ACHOT6_001941"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFP9947167.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVTHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRVMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALAHDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     WSTLDGISRE"
     gene            3106..3711
                     /gene="ynbA"
                     /locus_tag="ACHOT6_001942"
     CDS             3106..3711
                     /gene="ynbA"
                     /locus_tag="ACHOT6_001942"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFP9947168.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGIRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLFMSAER"
     gene            3711..4607
                     /gene="ynbB"
                     /locus_tag="ACHOT6_001943"
     CDS             3711..4607
                     /gene="ynbB"
                     /locus_tag="ACHOT6_001943"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFP9947169.1"
                     /translation="MLEKSLATLFALLILATLINRFLLWRLPERKGGEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISIHFPRWLYWVIPLNYLLIGFNC
                     FELFLLFIPLSGFLILATWRVFVGEPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPS
                     MNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            4623..6380
                     /gene="ynbC"
                     /locus_tag="ACHOT6_001944"
                     /pseudo
     CDS             4623..6380
                     /gene="ynbC"
                     /locus_tag="ACHOT6_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
     gene            6394..7686
                     /gene="ynbD"
                     /locus_tag="ACHOT6_001945"
                     /pseudo
     CDS             6394..7686
                     /gene="ynbD"
                     /locus_tag="ACHOT6_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415929.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
     gene            complement(7740..8345)
                     /gene="azoR"
                     /locus_tag="ACHOT6_001946"
     CDS             complement(7740..8345)
                     /gene="azoR"
                     /locus_tag="ACHOT6_001946"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFP9947170.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            8546..12448
                     /gene="hrpA"
                     /locus_tag="ACHOT6_001947"
     CDS             8546..12448
                     /gene="hrpA"
                     /locus_tag="ACHOT6_001947"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFP9947171.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELIIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMLEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            12720..13520
                     /gene="ydcF"
                     /locus_tag="ACHOT6_001948"
     CDS             12720..13520
                     /gene="ydcF"
                     /locus_tag="ACHOT6_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFP9947172.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LHDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            13718..15157
                     /gene="aldA"
                     /locus_tag="ACHOT6_001949"
     CDS             13718..15157
                     /gene="aldA"
                     /locus_tag="ACHOT6_001949"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFP9947173.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERIYVQKGIYDQFVNRLGETMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15199..16200)
                     /gene="gap"
                     /locus_tag="ACHOT6_001950"
     CDS             complement(15199..16200)
                     /gene="gap"
                     /locus_tag="ACHOT6_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFP9947174.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            16389..16919
                     /gene="cybB"
                     /locus_tag="ACHOT6_001951"
     CDS             16389..16919
                     /gene="cybB"
                     /locus_tag="ACHOT6_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFP9947175.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALVHHY
                     FWKDNTLLRMMPRKRS"
     gene            17164..17337
                     /locus_tag="ACHOT6_001952"
     CDS             17164..17337
                     /locus_tag="ACHOT6_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947176.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            17363..17431
                     /gene="yncP"
                     /locus_tag="ACHOT6_001953"
     CDS             17363..17431
                     /gene="yncP"
                     /locus_tag="ACHOT6_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFP9947177.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(17409..17558)
                     /gene="hokB"
                     /locus_tag="ACHOT6_001954"
     CDS             complement(17409..17558)
                     /gene="hokB"
                     /locus_tag="ACHOT6_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFP9947178.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            17957..19597
                     /gene="trg"
                     /locus_tag="ACHOT6_001955"
     CDS             17957..19597
                     /gene="trg"
                     /locus_tag="ACHOT6_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFP9947179.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRILASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(19635..20558)
                     /gene="ydcI"
                     /locus_tag="ACHOT6_001956"
     CDS             complement(19635..20558)
                     /gene="ydcI"
                     /locus_tag="ACHOT6_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9947180.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            20775..22118
                     /gene="ydcJ"
                     /locus_tag="ACHOT6_001957"
     CDS             20775..22118
                     /gene="ydcJ"
                     /locus_tag="ACHOT6_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9947181.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            22343..23998
                     /gene="opgD"
                     /locus_tag="ACHOT6_001958"
                     /gene_synonym="mdoD"
     CDS             22343..23998
                     /gene="opgD"
                     /locus_tag="ACHOT6_001958"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFP9947182.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            24138..24362
                     /gene="ydcH"
                     /locus_tag="ACHOT6_001959"
     CDS             24138..24362
                     /gene="ydcH"
                     /locus_tag="ACHOT6_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFP9947183.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            24425..24961
                     /gene="rimL"
                     /locus_tag="ACHOT6_001960"
     CDS             24425..24961
                     /gene="rimL"
                     /locus_tag="ACHOT6_001960"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFP9947184.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLSKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(24956..25936)
                     /gene="ydcK"
                     /locus_tag="ACHOT6_001961"
     CDS             complement(24956..25936)
                     /gene="ydcK"
                     /locus_tag="ACHOT6_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFP9947185.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            26060..27052
                     /gene="tehA"
                     /locus_tag="ACHOT6_001962"
     CDS             26060..27052
                     /gene="tehA"
                     /locus_tag="ACHOT6_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFP9947186.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVMSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            27049..27642
                     /gene="tehB"
                     /locus_tag="ACHOT6_001963"
     CDS             27049..27642
                     /gene="tehB"
                     /locus_tag="ACHOT6_001963"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFP9947187.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            27944..28612
                     /gene="ydcL"
                     /locus_tag="ACHOT6_001964"
     CDS             27944..28612
                     /gene="ydcL"
                     /locus_tag="ACHOT6_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFP9947188.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(28630..>28787)
                     /locus_tag="ACHOT6_001965"
     CDS             complement(28630..>28787)
                     /locus_tag="ACHOT6_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFP9947189.1"
                     /translation="GYFASSCGGAPISVIRQYIEQQQTPGQVENRALYPRPEGWGFTA
                     HRIKSAI"
BASE COUNT         7108 a         6687 c         7514 g         7478 t
ORIGIN      
        1 taccgatgtg atgatcgtta actgtggcag tatcccgcgt tcagaaggca aggcgtgtcg
       61 ggtgtttgat ctgcgcaata ttgttggtgc ctgacgtgtc gtctggccct ggtggggtaa
      121 agcgccaggg ccagaagtcg atacgacctg tgctatgatt cataaatcac aacaataaca
      181 acagactgaa tcgaatgagt aaacttgata cttttatcca acatgctgta aacgctgttc
      241 ctgtcagtgg cacatctttg atctcctctc tgtatggtga ttcgctttcc caccgtggtg
      301 gtgaaatctg gctgggtagt ctggctgctt tgctggaagg gctgggattt ggtgagcgtt
      361 tcgtccgtac cgctttgttt cgtcttaata aagaaggctg gctggatgtt tcccgcatcg
      421 ggcgacgcag tttctatagc ctcagtgata aaggcttacg cctgacgcga cgggcagaaa
      481 gtaaaattta tcgcgcagag caacctgcat gggatggtaa atggctcctg ttgctctcgg
      541 aaggtttaga taaatcaacg ctggctgatg tcaaaaagca gttgatctgg caaggttttg
      601 gcgcactggc acccagcctg atggcatcgc cgtcgcaaaa actggcggat gtacagacac
      661 ttttgcatga agcgggggtg gcggataacg tgatttgttt tgaagcgcaa ataccactgg
      721 cgctttctcg tgcagcactg cgtgccagag tggaagagtg ctggcattta actgaacaaa
      781 atgccatgta cgaaaccttt attcagtcat tccgcccgct ggtgccgctg ttaaaagagg
      841 cagcagacga gttaaccccg gagcgtgcat ttcatattca gcttttactg atccattttt
      901 atcgccgtgt cgtccttaaa gacccattgt tgccagagga gttgcttccg gcgcactggg
      961 cagggcatac ggcgcgtcag ctgtgtatca acatttatca gcgcgtagcg cctgctgctt
     1021 tagcgttcgt tagtgaaaaa ggtgaaacct cggtcggtga actgcctgcg ccgggaagcc
     1081 tgtattttca acgttttggc ggtttgaata ttgaacagga ggcgttatgc caatttacca
     1141 gatagatggt ctgactccgg ttgtgccaga agagagtttt gtccatccga cagcggtatt
     1201 gatcggcgat gttattctcg gcaagggcgt ttacgttggg ccaaatgcca gtctgcgtgg
     1261 cgattttggt cgtatcgtag tgaaagatgg cgcgaacatt caggataatt gcgttatgca
     1321 cggttttccc gaacaggata ctgtcgtgga agaagatggg catattggtc atagcgctat
     1381 ccttcatggt tgcattatcc gccgcaatgc attagtggga atgaacgcgg tagtgatgga
     1441 cggcgcggtg attggcgaga acagcattgt tggcgcatcc gcatttgtga aagccaaagc
     1501 agaaatgcct gccaattacc tgattgtcgg cagcccggcg aaagcgattc gtgaactcag
     1561 tgagcaggag ctggcatgga aaaagcaggg tacgcatgag tatcaggtgc tggttactcg
     1621 ttgtaagcag acgttgcatc aagttgagcc attgcgggaa gttgaaccga acaggaaacg
     1681 tctggtattt gatgagaatc tgcgaccgaa acagtaacgt ccagatactg aacattctaa
     1741 tttttagcat gtgataccta aaccgcaacg ctgatacagg ttgcggtttt ttattgccgg
     1801 atgcggtact tgacgcgttt tgttttgtgt ttttcaggac aatagagcaa ctcatccagt
     1861 aatcttgttt acgccttcgc attatttatc tcttttcgtt tctatactga tttttctcaa
     1921 tccgttttat tacagggcag ggtgcgatga gcagcaatac atttactctc ggtacaaaat
     1981 ccgttaaccg tcttggttat ggcgcgatgc aactggcagg tcctggggtt tttggtcccc
     2041 caagagatcg ccacgtcgct ataactgtgc tgcgtgaggc gctggcattg ggcgtcactc
     2101 acattgatac cagcgacttt tatggtccgc acgtcaccaa tcagattatc cgcgaagcgc
     2161 tttatcctta ctctgacgac ctgacaattg tcactaaaat tggtgcgcgg cgtggagagg
     2221 acgcttcctg gttgcccgca ttttctccgg cagagctgca aaaagcggtg cacgataatc
     2281 tacgtaatct cgggctggac gtgctggatg tggttaacct gcgcgttatg atgggggatg
     2341 gtcatggccc agcggaagga tcgattgagg ccagcctgac cgtgctggca gagatgcaac
     2401 aacaaggcct ggtaaaacat attggcctga gcaatgtcac accgacgcag gttgcagagg
     2461 cgcgcaagat tgccgaaatt gtctgtgtgc aaaacgaata caacatcgcg caccgtgctg
     2521 atgatgcaat gattgatgct ttggcccacg atggcattgc ctacgtgccg ttcttcccgc
     2581 tcgggggctt tacaccgctg caatcgtcca cactttccga tgttgctgcg agcctgggtg
     2641 caacacccat gcaggtggcg ctggcgtggc tgttacaacg ttcaccgaat attttgctga
     2701 tcccagggac gtcttctgtt gcgcatttac gggagaatat ggctgctgaa aaattgcatc
     2761 tttctgagga agtgtggtct acgttggatg gtatttcgcg agaataacga atatacaaaa
     2821 gggaaagatg catttccctt ttgtttcttt tttaatggca tggagtgcat atgtttaagg
     2881 ccaggatttg cggctggata atcagggaaa aggcaatgga ttcattgatt cgtacagtga
     2941 tcgatggtat ctcactgaca tccagtcggt aatcaaacag atgtgtagga aaaccatcca
     3001 ccacatcttg atatattccg aatttacgtt gatgctccgc gttgtgatat ctccgccatc
     3061 acaattgatg caccatagag caaactgaat atcacggaat gataaatgac gctctaccag
     3121 ataaaaccgc tctttcagtc gctgttaagg ccgacgatgt tttggcttta taagcaccac
     3181 gttacagcga atcacatcac tcttacggca ctggcgcttt ctctttttac cggattgctg
     3241 ttggtgttgg tggcacaacc catcctcttt ttgctattgc ccatcgtgct ttttatccgt
     3301 atggcgctca atgcgctgga tggcatgttg gcgcgtgagt gcaatcagca aacacgtctg
     3361 ggggcgattt tgaatgaaac tggcgatgtt atttccgata ttgcgctcta tttaccgttt
     3421 ttatttttac cggaaagtaa cgcatcactc gtaatactca tgttattttg caccatattg
     3481 actgagtttt gtggtttact cgcgcagaca attaacggta ttcgcagtta tgccggtcca
     3541 ttcggcaaaa gcgatcgtgc gttaatattt gggttgtggg ggctggccgt tgccatttat
     3601 ccacagtgga tgcaatggaa taatctttta tggagcattg cttcaattct gcttctctgg
     3661 actgcgatta atcgttgtcg gagtgtgctt tttatgagtg ctgaaagata atgctggaaa
     3721 aatctctggc aacacttttc gcgttgttaa ttttagcaac gctgataaac cgttttctgc
     3781 tatggcggtt accggagaga aaagggggtg aggttacatt acgtattcgt acctggtggg
     3841 gcatcgtcat ttgtttttca ctggtgattt caggtccacg ctggatgacg ttgacgtttt
     3901 ttgcgctgat aagttttctg gcattgaaag aatattgtac gcttatatcc atacattttc
     3961 cgcggtggtt atattgggtt attcctctta actatttgct tatcggtttt aattgctttg
     4021 agctgtttct gttatttata cctttgtctg ggtttctgat attagccacc tggcgagttt
     4081 ttgtgggtga accctccggt tttctgcata ccgtgagcgc tattttttgg ggctggataa
     4141 tgaccgtttt cgccttgagt catgccgcct ggttattaat gttgccatcc atgaatatcc
     4201 agggcggggc gttactggtg ttatttcttt tggcgttaac cgagtcaaac gatattgcac
     4261 agtatttatg ggggaaatcc tgcggcagaa gaaaagtggt ccccaaagtc agcccgggaa
     4321 aaacgctgga aggtctggtg gggggcgtca tcaccaccat gattgcgtca ctgattattg
     4381 gaccgttgct gacaccgcta aatacattac aggcattatt agcgggtttg ttaattggta
     4441 ttagtggttt ttgcggcgat gtagtgatgt cagccatcaa acgagatatt ggtgttaaag
     4501 atagtgggaa actattacca ggacatggcg gactccttga caggattgac tcattaattt
     4561 tcaccgctcc ggtatttttt tattttatac gctactgctg ttactgaagg aaaatagaga
     4621 aaatggaaaa ttcacgcatc cctggggaac atttttttac caccagtgat aatacagcgt
     4681 tgttttatcg gcactggccc gctttacagc ccggggcgaa aaaggtcatc gtcttatttc
     4741 atcgcgggca tgaacattct ggtcgtctac aacatctcgt tgatgaactg gcgatgccag
     4801 atactgcttt ttatgcctgg gatgcccgag ggcatggaaa aagttcgggg ccgcgtggtt
     4861 atagcccatc tcttgcgcgt tcagtgcggg atgtcgatga atttgtccgt tttgctgcca
     4921 gcgacagcca ggttggactg gaagaggtgg ttgtgatcgc gcaaagcgtc ggcgcagtgc
     4981 tggttgccac atgggttcat gattatgcgc ctgcaattcg cgggctggtg ctggcttctc
     5041 cggcctttaa ggttaaattg tatgtgccgc tggcacgtcc tgcgctgacg ctatggcatc
     5101 gtctgcgtgg tctgtttttt attaattcct atgtgaaagg acgctatttg acccacgatc
     5161 ggcaacgggt ggcgagtttc aataatgatc cgctgatcac acgggcgatt gccgtcaata
     5221 tcttgctcga tctctataaa acgtctgaac gtattattag agatgcggcg gcgattacgc
     5281 tccccacgca acttctgata tcaggcgatg actatgtggt gcatcgccaa ccgcagattg
     5341 atttttatca gagattacgt agccctctga aagagctgca tctgctgcca ggcttttatc
     5401 acgacacgtt gggtgaagag aacagggcgc aggcatttga aaaaatgcaa agctttatta
     5461 gtcgtttata tgctaacaag tcacaaaaat ttgattatca gcatgaagac cgcactggac
     5521 catcagcgga tcgctggcgc ctcctttcag gtggacccgt gccattatcg ccggttgatt
     5581 tggcgtatcg ctttatgcgt aaagcgatga aattgttcgg gacgcactct gcgggcctgc
     5641 atctcggaat gagcactggc tttgattcag gcagttcgct ggattatgtc tatcaaaatc
     5701 aaccgcaagg cagtaacgca ttcgggcgct taatcgacaa aatctacctg aacagtgttg
     5761 gctggcgcgg tattcgccag cgcaaaaccc atttataaat actgattaaa caagccgttg
     5821 ccgatctcca cgccaaaggt ttagccgtcc gcgtggttga cattgccgca gggcatgggc
     5881 gctatgtact ggatgcactg gcaaacgagc ctgccgtaag cgatatttta ttacgtgatt
     5941 acagtgagtt aaatgttgca caggggcaag agatgattgc ccaacgggga atgtctgggc
     6001 gggtgcgttt tgaacagggc gatgcgttta acccggagga actcagcgcg ttaactccgc
     6061 ggcctacgct ggcgattgtc tctggactgt atgagctgtt tcccgaaaat gagcaggtaa
     6121 aaaactcact cgcaggtctt gccaatgcca tcgaaccggg cggcattctc atctacaccg
     6181 ggcagccgtg gcaccctcaa ctggagatga ttgccggggt gttaaccagt cataaagatg
     6241 gtaaaccgtg ggtaatgcgc gtgcgttcgc aaggggagat ggattcactc gtgcgtgatg
     6301 ccggatttga taaatgcaca caacggattg atgagtgggg tatttttacg gtttcgatgg
     6361 cggtgcgtcg tgataactga acgtcggaac gtattgctac aaggcgctgg ctggttattg
     6421 ttgctggccc cgtttttctt cttcacctat ggatctctta atcagttcac cgcggttcag
     6481 gaccttaaca gccatgatat ccccagtcag gtattcggtt gggaaacggc gatccctttt
     6541 cttccctgga ctattgttcc ttactggagt ctggatcttt tatatggatt ttcgctgttc
     6601 gtttgtagca cgacattcga acagcgccga cttgtccacc ggcttattct ggcaacggta
     6661 atggcctgct gcggtttttt gctctatccg ctgaagttta gttttatccg tcctgaagtg
     6721 agtggggtga cgggatggct attttcgcaa cttgaactgt ttgatctgcc ttataaccag
     6781 tctccttcgc tgcatattat tctctgctgg ctactttggc gtcactttcg tcagcatctg
     6841 gctgagaggt ggcgtaaagt ctgtggcgga tggtttttac tcatcgctat ttctactctg
     6901 acgacctggc agcatcattt tattgatgtc atcacagggc tggcggtagg tatgttgatt
     6961 gactggatgg tgcccgtcga tcgtcgttgg aattatcaga aacctgatca acgtcgaatc
     7021 aaaatagcac tgccatatgt cgtaggcgcg ggctcgtgca ttgtgttgat ggagctaatg
     7081 gtgatgattc agttatggtg gtcagtctgg ttatgttggc cagtattatc gctactcatt
     7141 attggccgtg ggtacggtgg gcttggcgcg ataacaacag ggaaagatag tcaggggaaa
     7201 ctcccgcccg ccgtttactg gctgacattg ccctggcgca tcgggatgtg actgtctatg
     7261 cgtttgtttt gtcgtcgcct ggagccggtg agcaaaatga ctgctggtgt ttatttaggg
     7321 gcgtttccac gacatattcc ggcacagaat gcggttctgg acgtcacctt tgaattccct
     7381 cgcggacgag cgacaaaaga tcgactctat ttctgtgtac cgatgctgga tctggtggtt
     7441 ccggaagagg gggagctccg acaggccgtg gcgatgctgg aaacattacg cgaagagcaa
     7501 ggcagcgttc tggtccattg cgcgttggga ttatcgcgca gtgcgctggt agtggcggca
     7561 tggctgttat gttacggaca ctgtaaaacc gttaatgaag cgattagcta tattcgagcc
     7621 agacgcccgc agattgtgct taaggaagag cacaaagcga tgctgaaatt atgggaaaac
     7681 aggtaagtgg attgagatgt ggactgaata tctacagtcc acatcaagac cgtgtccggt
     7741 tatgcagcaa caatgctgtc gatggctgct ttcgcgtcag actgcgcttt cgctgccatt
     7801 tccggaccgt atgcgatccc ttcggcgaag acaaatttca catcggtaat gccgataaag
     7861 ccgaggaacg tggacagata cggcgtcacc aggtccgttg gtccatcttt gtggatccca
     7921 ccgcggctgg taataacgat ggctttttta cccgttacca gaccttccgg accgttctcg
     7981 gtatagcgga aagtaacgcc tgcgcgtgca accaggtcaa aatagttttt caactgagtc
     8041 gagatgttga agttatacat cggtgccgca ataacgataa cgtcgtgggc tttcagctcg
     8101 gcaatcagct catcggaaag cgccagagct tcctgctgac gcggagtcag cggcgcatcg
     8161 ctcggacgca gagcgccaac cagttcgcca tccagtaccg gaatcggatt tgcagccagg
     8221 tcgcgaacgg tgatttcatc agcggagtgc ttttcgcgcc attgttcaac aaaataatcg
     8281 gacaactgat tagactgaga gtaccctgcc aggatgctgg atttaagaac taataccttg
     8341 ctcatggtgt ttccttatag atgtttgaat gggcgatgcc ccgttgcttg ctgacacttt
     8401 attcacaatc ctgccacaga gatagcgcaa taaatcgaag cctatgttcg aatttattga
     8461 acaacgcata gaaagccgcg atgtggtact ctatatctat catttaaaag aaaattaatc
     8521 aggcagacta ctgcccacta acgttatgac agaacaacaa aaattgacct ttactgcttt
     8581 gcagcagcgg ctggattcgc tgatgctgcg cgacagactg cgtttttctc ggcgtctgca
     8641 cggtgttaag aaggttaaaa atcctgatgc acaacaggcc attttccagg agatggcgaa
     8701 agagattgac caggcggcag ggaaagtcct gctgcgtgaa gcggcacgac cggaaatcac
     8761 ttatcctgac aatttaccgg ttagtcagaa aaaacaggac atactcgaag cgattcgtga
     8821 tcaccaggtg gtgatcgtcg ccggggaaac cggttcaggc aaaaccaccc agttgccgaa
     8881 aatctgtatg gagctggggc gcgggattaa aggactgatc ggccataccc agccgcgtcg
     8941 cctggcggca agaacggtgg cgaaccgtat tgcggaagag ctgaaaacgg agccgggcgg
     9001 ttgcatcggt tacaaagtgc gtttcagcga tcacgttagt gataacacga tggtcaagct
     9061 gatgaccgac ggtatcctgc tggcggagat ccagcaagac cgcctgctga tgcagtacga
     9121 caccatcatt attgacgaag cacacgaacg cagcctgaat atcgattttc tgctcggcta
     9181 tttgaaagag ttgctgccgc ggcgtcctga cctaaaaatc attatcactt ccgcgactat
     9241 cgacccggaa cgcttttcgc gccactttaa taatgcgccg attatcgaag tctccggtcg
     9301 gacctatccg gtggaagtgc gctatcgacc gattgttgaa gaagccgacg acaccgagcg
     9361 cgatcagttg caggcgattt ttgacgccgt agacgaactg agtcaggaaa gccctggcga
     9421 cattctgatc tttatgagtg gtgagcggga aattcgcgat accgccgatg cgctgaacaa
     9481 gctaaatctg cgtcataccg aaatcttgcc gctttatgcg cggctttcga acagcgaaca
     9541 gaaccgcgtg ttccagtcgc acagcggacg gcgcattgtg ctggcgacca acgtcgcgga
     9601 aacctcgctg accgtaccgg ggattaaata cgttatcgac cccggtacag cacgtatcag
     9661 ccgctacagc tatcgcacca aagtgcagcg tttgccgatt gaacccattt cgcaggcgtc
     9721 tgccaatcag cgtaaaggcc gctgtgggcg tgtgtccgaa gggatctgta ttcgtctcta
     9781 ttccgaagac gatttccttt cgcgcccgga gtttaccgat ccggagattc tgcgtaccaa
     9841 cctggcctcg gttattttgc agatgaccgc gctggggctg ggcgatatcg ctgcgttccc
     9901 gtttgtcgaa gcaccggata aacgcaatat ccaggatggc gtgcgtctgc tcgaagaact
     9961 gggcgcgatc accacggatg aacaggccag cgcctataaa ctgacgccgc tcggtcgcca
    10021 gctctcgcag ttgcctgtcg acccacgtct ggcgcgtatg gtgctggaag cgcaaaaaca
    10081 tggctgcgtg cgtgaggcga tgattatcac gtccgcgctt tccattcagg atccgcgcga
    10141 gcgtccgatg gataaacagc aggcatcgga cgaaaaacat cgtcgcttcc acgacaaaga
    10201 gtctgacttc ctcgcgtttg tgaatctgtg gaattatctt ggcgagcagc aaaaggcgct
    10261 ttcttccaac gccttccgtc gcctgtgtcg taccgattat ctcaactatc tgcgcgtgcg
    10321 cgaatggcag gatatctaca cccagttgcg ccaggtggtg aaagaacttg gcattccggt
    10381 taacagcgaa ccggcggagt atcgcgaaat tcacattgcg ttgctgacgg gtttactttc
    10441 ccatatcggc atgaaagatg ccgataaaca agaatatacc ggcgcacgta acgcgcgttt
    10501 ctccatcttt cccggttctg gtttattcaa aaaaccgccg aaatgggtaa tggtggcgga
    10561 actggtagaa accagccgcc tgtgggggcg cattgctgcg cgtatcgacc cggaatgggt
    10621 ggagcctgtc gctcagcatt tgattaaacg cacctacagc gaacctcact gggaacgggc
    10681 gcagggcgcg gtgatggcaa cggaaaaagt cactgtttat ggtttgccga ttgttgccgc
    10741 gcgcaaggtc aactacagcc agatcgatcc ggcgttatgc cgtgaactct ttattcgcca
    10801 tgcgctggtg gaaggtgact ggcagacgcg tcacgcattc ttccgtgaaa acctgaaact
    10861 gcgggccgaa gtggaagagc tggaacacaa atcacgtcgc cgcgatattc tggttgatga
    10921 cgaaacgttg tttgagttct acgaccagcg catcagccac gatgtgatct ctgcacgcca
    10981 cttcgatagc tggtggaaaa aagtcagccg cgaaacgcct gatttgctca actttgaaaa
    11041 gagcatgttg atcaaagaag gggcggaaaa aatcagcaag ctggattacc cgaacttctg
    11101 gcatcagggc aatctcaagc tgcgtttgag ctatcagttt gagcccggcg cggatgctga
    11161 cggtgtgacg gtgcatatcc cgctgccgtt acttaaccag gttgaggaga gcgggtttga
    11221 gtggcagatc cccggcctgc gtcgtgagct gatcatcgcc ctgattaaat cactgccaaa
    11281 gccggtacgc cgtaattttg tgcccgcacc aaactacgcc gaagcgtttt taggccgcgt
    11341 cacaccgctg gagttaccgt tgctcgacag ccttgagcgc gagttacggc ggatgaccgg
    11401 tgttaccgtt gaccgcgaag actggcactg ggatcaggtg cccgatcacc tgaaaatcac
    11461 cttccgcgtg gtggacgaca aaaataagaa gctaaaagaa gggcgctcat tacaggatct
    11521 gaaagatgcg ctgaaaggca aagtgcagga aacgctgtcc gcggtggcgg atgacggtat
    11581 cgagcagagc gggctgcata tctggagctt tgggcaactg ccggaaagct acgaacagaa
    11641 gcgcggcaac tataaagtga aggcgtggcc ggcgctggtg gatgaacgtg acagtgtggc
    11701 gatcaaactg tttgataacc cgctggagca aaaacaggca atgtggaacg gtcttcgccg
    11761 tctactgctg ctaaatattc cctcgccgat caagtattta catgagaagt taccaaacaa
    11821 agccaagctt ggactgtact ttaacccgta tggcaaagtg ctggagctga tcgacgactg
    11881 tatctcctgc ggtgtggata aattgatcga cgccaatggt ggcccggtct ggacggaaga
    11941 aggctttgct gcgctgcatg aaaaagtgcg cgccgaactg aatgacacgg tggtggatat
    12001 tgctaagcag gtcgagcaaa tcctcactgc ggtgttcaat atcaacaaac gtctgaaagg
    12061 gcgggtggat atgaccatgg cgctggggct ttctgacatt aaggcgcaga tgggcgggtt
    12121 ggtgtatcgc ggttttgtca ctggtaacgg cttcaaacgg ctgggcgaca cgctgcgtta
    12181 tttgcaggcg attgaaaaac ggctggaaaa actggcggtt gatccgcatc gcgaccgtgc
    12241 tcagatgctg aaagtcgaaa acgtccagca ggcgtggcag caatggatca acaaactgcc
    12301 gcccgcacgt cgtgaggatg aagacgtgaa agagatccgt tggatgttag aagagttgcg
    12361 cgttagttac ttcgctcaac aacttggtac gccttatccg atttcagata agcgtatttt
    12421 gcaggcgatg gaacagatta gcggttaacg cagctatttg ccagataaat ctaaaacccg
    12481 gtaagcattt agcgccgggt tttttattaa ttctaaaacg gtaagcgaaa aaattcagga
    12541 attcagaaaa atacaattct ctgctgcaag atgaataatg tttatctaca gcatttcctt
    12601 aaacgatatg tcaggcttgc ggagtggcgg ttaaggaaat acgacttcct cctttcagag
    12661 tgctccgctt ctcactatta tctcacgcag tattcttaag ggaacgataa ggaggagcca
    12721 tgaacattac cccgtttccg acgctttcgc cggcaaccat agatgctata aatgttatcg
    12781 gacagtggct ggcgcaggat gatttctccg gtgaggtgcc gtatcaggcc gattgcgtga
    12841 tccttgcagg caatgcggtt atgccgacta tcgacgcggc atgtaagatt gcccgcgatc
    12901 agcaaattcc tttactgatt agtggtggta tcggtcactc gacaactttt ttgtatagcg
    12961 ccatcgcaca gcatccgcac tacaacacta tccgcaccac tggcagagca gaagcgacca
    13021 tcctggcgga tatcgctcat cagttctggc acattccgca tgaaaaaatc tggattgaag
    13081 accagtcaac aaactgcggt gaaaacgcac gctttagcat cgcgctattg aatcaggccg
    13141 tagaacgagt tcatacggct atcgttgtac aggaccccac catgcagcgg cgcacgatgg
    13201 caacgttccg ccgtatgacc ggggacaatc ccgatgcacc acgctggtta agttatcccg
    13261 gattcgttcc tcagttagga aataacgcag acagtgtaat ctttattaat cagttacaag
    13321 gattatggcc ggttgagcgt tatctctcac tactcactgg cgagctgccg cgtttacacg
    13381 atgatagcga tggctacggt ccccgcgggc gagattttat cgttcacgtt gattttccgg
    13441 cagaagtcat ccatgcatgg caaacgctga aacatgatgc ggtgctcatc gaggcgatgg
    13501 aaagtcgctc gttacgttaa aaattgcccg tttgtgaacc acttgtttgc aaacgggcat
    13561 gattcctgac ttttatttct gccttttatt ccttttacac ttgtttttat gaagcccttc
    13621 acagaattgt cctttcacga tgccgtcatc tctgatgatt gatgttaatt aacaatgcat
    13681 tcaccgaaaa caaacatata aatcacagga gtcgcccatg tcagtacccg ttcaacatcc
    13741 tatgtatatc gatggacagt ttgttacctg gcgtggagac gcatggattg atgtggtaaa
    13801 ccctgctaca gaggctgtca tttcccgcat acccgatggt caggccgagg atgcccgtaa
    13861 ggcaatcgat gcagcagaac gtgcacaacc agaatgggaa gcgttgcctg ctattgaacg
    13921 cgccagttgg ttgcgcaaaa tctccgccgg gatccgcgaa cgcgccagtg aaatcagtgc
    13981 gctgattgtt gaagaagggg gcaagatcca gcagctggct gaagtcgaag tggcttttac
    14041 tgccgactat atcgattaca tggcggagtg ggcacggcgt tacgagggcg agattattca
    14101 aagcgatcgt ccaggagaaa atattctttt gtttaaacgt gcgcttggtg tgactaccgg
    14161 cattctgccg tggaacttcc cgttcttcct cattgcccgc aaaatggctc ccgctctttt
    14221 gaccggtaat accatcgtca ttaaacctag tgaatttacg ccaaacaatg cgattgcatt
    14281 cgccaaaatc gtcgatgaaa taggccttcc gcgcggcgtg tttaaccttg tactggggcg
    14341 tggtgaaacc gttgggcaag aactggcggg taacccaaag gtcgcaatgg tcagtatgac
    14401 aggcagcgtc tctgcaggtg agaagatcat ggcgactgcg gcgaaaaaca tcaccaaagt
    14461 gtgtctggaa ttggggggta aagcaccagc tatcgtaatg gacgatgccg atcttgaact
    14521 ggcagtcaaa gccatcgttg attcacgcgt cattaatagt gggcaagtgt gtaactgtgc
    14581 agaacgtatt tatgtacaga aaggcattta tgatcagttc gtcaatcggc tgggtgaaac
    14641 gatgcaggcg gttcaatttg gtaaccccgc tgaacgcaac gacattgcga tggggccgtt
    14701 gattaacgcc gcggcgctgg aaagggttga gcaaaaagtg gcgcgcgcag tagaagaagg
    14761 ggcgagagtg gcgttgggtg gcaaagcggt agaggggaaa ggatattatt atccgccgac
    14821 attgctgctg gatgttcgcc aggaaatgtc gattatgcat gaggaaacct ttggcccggt
    14881 tctgccggtg gtcgcatttg acacgctgga agaggctatc tcaatggcta atgacagtga
    14941 ttacggcctg acctcatcaa tctataccca aaatctgaac gtcgcgatga aagccattaa
    15001 agggctgaag tttggtgaaa cttacatcaa ccgtgaaaac ttcgaagcta tgcaaggctt
    15061 ccacgccgga tggcgtaaat ccggtattgg cggcgcagat ggtaaacatg gcctgcatga
    15121 atatctgcag acccaggtgg tttatttaca gtcgtaatga gtaaaagagg cggaggtttt
    15181 ttcctccgcc tgtgcgcgtc agagtttagc gaatttttcg agggtgcgaa taagctgcgt
    15241 gacgaagcca tattcgttat cgtaccaggc gaccgttttc accagttgta aatcgcccac
    15301 ggcggtaatt tccgtttgcg tggcatcaaa caccgaaccg aaatggctgc caatgatatc
    15361 ggaagagact atttcttcat cggtataacc aaatgactcg ttattggtgg ttgcttgttt
    15421 aagtgcgtta ttcacctctt cggcagtcac tttttttccg agaatcgata ccagttcagt
    15481 gaccgaacct gttttcaccg gcacgcgttg cgcatgacct ttcagtttgc cgctcagttc
    15541 cgggatcacc agaccaatgg cttttgccgc ccccgtagtg tggggaatga tattttctgc
    15601 cgctgcgcgt gaagcacgta aatctttacc acgtgggcca tccaccagtg actgggtgcc
    15661 agtataggca tgaatggtcg tcatcgtgcc gacttctatc ccgaaactgt catgcaaggc
    15721 tttggccatc ggcgcaagac agttagtggt gcatgacgcc acggaaacaa tggtgtcgtt
    15781 gccatccaga gtgtcgtcat tgacgttata aacgatagtt ttcatttcac cggcaggggc
    15841 ggaaatcaac accttcttcg caccagcatc aagatgcgcc tgcgatttct cggcggaggt
    15901 ataaaagcca gtacattcga caatgatttc tgcacctttc gctttccacg gaatattttt
    15961 agcctctttt tcggcgtaaa ccgcgatact tttcccatca acgataagtg aatcttccgt
    16021 aaaatcaacg ctccagggga atggtccgta gtttgaatca tgtttcagca ggtaggcgag
    16081 aatttttggg gaagtgagat cattaatagc gacaacgtct atgttgcttt tgacttcaag
    16141 taatcgacgc aacaccagtc gaccgatacg accaaaaccg ttaataccaa ctttactcat
    16201 ggttttctcc tgtcaggaac attcggatga aaattgatcc tttccaggct tagaccagga
    16261 ttgtgggatg ggcaatctcc attcttacag tgaaacgtaa cgtactgaaa acgggtgaac
    16321 aatatttaat gaaattttga gaaaagcccg ttatgttaac ggaaaattat gttaaagcag
    16381 gaaatgttat ggaaaataaa tactcaaggt tacaaatcag cattcactgg ctggtctttt
    16441 tgctggttat cgccgcgtat tgcgcaatgg agtttcgggg tttcttccca cgtagcgatc
    16501 ggccactcat caacatgatt catgtttcct gcggcatctc aattctcgtg ctgatggtcg
    16561 ttcgtctttt gttaaggctg aaatacccaa ccccgccgat tatacctaag ccaaaaccga
    16621 tgatgacggg actggcgcat ttgggacatt tggtgattta tctcctgttt attgcgctgc
    16681 cagtgattgg tttggtgatg atgtataacc ggggcaaccc gtggtttgcg tttggtttga
    16741 cgatgcctta cgcttcagag gccaatttcg aacgggttga tagcttaaag tcgtggcatg
    16801 aaacgctggc gaatctggga tattttgtca tcgggttgca cgctgcggct gcactggtac
    16861 accactattt ctggaaggac aacacacttc tacgcatgat gccgcgtaaa cgttcctgaa
    16921 ggatatttaa agaaaacgcc tgtactaaaa ccgacccgtg gtacaggcga agaatacggg
    16981 tctacatcgg aagcgcctat tatatttatt tgtatgacaa ataaaaccat aatccttgcc
    17041 catacgtcca tctggcttat ttttaatcaa ttcacccgat ctttgatctc atcaacggta
    17101 tcaaaataaa ccagcgtata acgttcaaat cgaacataag attcgataaa ccatggaggt
    17161 tatatgaaaa aactggcact tattttgttt atgggaacgc ttgtttcctt ttatgccgat
    17221 gccgggcgca aaccctgttc tggttcgaaa ggggggatct cacactgtac ggcaggcggc
    17281 aaatttgtct gtaatgatgg ttctattagt gcctcgaaaa aaacatgcac taactgaagt
    17341 gtaaaagggg tgccatgaga aaatgaatct gctagtcaga tgcgcgggga aaatccccgc
    17401 gcttgccctt acctggacgt gcaggccatg agcgcagcaa cctccttatc accgtcccgg
    17461 aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga atgtcagaat cgtaatgcag
    17521 ataatgagca gacacaccac cagagggttg tgcttcatag cctttctcct tgccggatgg
    17581 cgggtaagag gctaagatct gaattgctag gttcattcgt tggcctcggt tgatagaaat
    17641 atcggtcggg gccttcgtct ttctgattcc cggttagcct gaaaacagaa agtctcaggc
    17701 acccgcaggc atcctatgag gtttccttag ggacgaaaat aatcacttca cgaaattgcg
    17761 tgctgttttc cagaattttt cgtcattcgg gttagccagt ttagccattc gttactctct
    17821 tcattccaat agcattaatt ttctctgcaa taattgttgt aaaaatgtga cgcaaagagg
    17881 tttttggtca taagtaatta ccgtcaagtg ccgatgactt tctatcagga gtaaacctgg
    17941 acgagagaca acggtaatga atacaactcc ctcacatcga ttaggttttt tgcatcacat
    18001 caggctggtt ccgttatttg cctgcattct aggcggtatc ttagttctat tcgcattaag
    18061 ttctgctctg gctggctatt tcctctggca ggccgatcgc gatcagcgtg atgttactgc
    18121 ggagattgag attcggaccg ggttagcgaa cagttcagat tttttgcgtt cagcccggat
    18181 caatatgatt caggccgggg ctgcgagtcg tattgcggaa atggaagcaa tgaagcgaaa
    18241 tattgcgcaa gccgaatcgg agattaaaca gtcgcagcaa ggttatcgtg cttatcagaa
    18301 tcgaccggtg aaaacacctg ctgatgaagc cctcgacact gaattaaatc aacgctttca
    18361 ggcttatatc acgggtatgc aacctatgtt gaaatatgcc aaaaatggca tgtttgaagc
    18421 gattatcaat catgaaagtg agcagatccg accgctggat aatgcttata ccgatatttt
    18481 gaacaaagcc gttaagatac gtagtaccag agccaaccaa ctggcggaac tggcccatca
    18541 gcgcacccgc ctgggtggga tgttcatgat tggcgcgttt gtgcttgccc tggtcatgac
    18601 gctgataaca tttatggtgc tacgtcggat cgtcattcgt ccactgcaac atgccgcaca
    18661 acggattgaa aaaatcgcta gtggcgatct gacgatgaag gatgaaccgg cgggtcgtaa
    18721 tgaaatcggt cgcttaagtc gtcatttaca gcaaatgcag cattcactgg ggatgacagt
    18781 agggactgtt cgacagggtg cggaagagat ttatcgtggc accagcgaaa tttcagctgg
    18841 caatgcggac ctgtcatctc gcaccgaaga acaagcggcg gctatcgaac aaactgccgc
    18901 tagcatggag caactcactg cgacggtgaa acagaatgcg gataacgcgc atcatgccag
    18961 caaactggcg caagaggctt ctattaaagc cagcgatggc gggcagacgg tttccggtgt
    19021 agtaaaaacg atgggcgcta tctctacaag ttcgaagaaa atttccgaga tcaccgccgt
    19081 catcaacagt attgctttcc agacgaatat tctggcactg aatgctgccg ttgaagccgc
    19141 gcgagcgggt gagcaaggcc gtggatttgc cgttgtcgcc agcgaagtac ggatactcgc
    19201 aagccgcagc gcccaagcgg cgaaagagat tgaaggcttg atcagtgaat cagtcaggtt
    19261 aattgacctg gggtcggatg aggtggcaac ggcagggaaa accatgagca ctattgttga
    19321 tgccgtcgcg agtgtcacac atatcatgca ggaaatcgcc gccgcctcgg atgaacaaag
    19381 tagaggcata actcaggtta gccaggcgat ttctgaaatg gataaggtga cgcaacagaa
    19441 tgcttctctg gtagaagagg cctcagcagc ggcggtgtcc cttgaagaac aggcggcacg
    19501 attaactgag gcggtggacg tattccgtct gaacaaacat tctgtgtcgg cagaacctcg
    19561 cggagcgggt gaaccagtta gtttcgctac ggtgtgaaaa tgttcaagga gggatcgaca
    19621 gatcccttca cctttcagaa cggcattgat tttcgaatag cgttaatcat caactggcaa
    19681 ccagaagaga acgtcgcatc tacgcgggtc agtattccaa tcggttcgcc tgcaccatgt
    19741 cccggaacag gcagggccac cagcgtggca tgacgcaggt cgtcttttac cgcgccagaa
    19801 gggacaaacc acacgtagtc gtattcaacc gtaagttgac gagatagcga agcagacagc
    19861 gtttcgatac aacccgaagg aattttacag ccctggctct gcactaatgc atctgaatgc
    19921 tggcgtggcg cagtgccttc tggtgataca acgaccggcc attccagcac ccggcttagc
    19981 gttacgttct cctgaagtag cgggtgatta gggcggacaa ccagcttcag cgattcaaga
    20041 aacagcagtt cgtaattaag cccggtcatc agttcaggat ctgacatccg accaatgccg
    20101 atatcgattt ccccggtttt taaacccgcc agaatcatag ggttactcat tgtcgcaact
    20161 tgcaaggtcg tctctttttg ttgctgatga aactgaccta taaccgaagg taatatcccc
    20221 agtgccgcag taggtagtgc accaaccctg acgacatcat tattaagatc ttctttacga
    20281 tgaagcgact gtccggcagt gttgatggcg tcaagaactc tgactgcatg cgttaaaaat
    20341 tgttcgccgg gtaaggtaag ttgcgccccc tgacgaccac gctcaaacaa gcgagcgcca
    20401 gtcagctgct ccagttcatt caatgtctta gagagcgcag gttgactcaa attaagggtt
    20461 tcagccgcgc gccccaaagt tccttgttgt gcgacagcta cgaatgtatg aaggtggcgc
    20521 aaacggatgc gctgactaaa cagactattt ttttccataa gcgatgttaa aaacgaagcg
    20581 gtgtcgctga caagtgaagt tgtttgatta tgataacttg attgcaaaat attattaaca
    20641 attaaagcaa ttatgttaca gcaaaatgga taatattgat gttttcgcgg cgagatcaca
    20701 gtttgtaaat tcttcccgcc agagtgaatg cggttaccta cactccagat tactgaccac
    20761 tggaggcaga cactatggcg aacagcatca cggcggatga gattcgggaa cagttttcgc
    20821 aggcaatgtc agccatgtac cagcaagaag ttccgcaata tggcacgctg ctggaactgg
    20881 tagctgatgt gaatctggct gtgctggaaa acaatcctca actgcacgaa aaaatggtaa
    20941 atgcagacga gctggcgcga ctgaatgttg aacgtcatgg ggcgattcgc gttgggactg
    21001 cacaagagct tgctactctt cggcggatgt ttgccattat ggggatgtac ccggtgagct
    21061 attacgatct ctcgcaggca ggggtgccgg tacattcgac agcatttcgg cccattgatg
    21121 atgcttctct ggcgcgtaat cccttccgcg tttttacctc cttactccgc cttgagctta
    21181 tcgagaacga aattttgcgc cagaaagcgg cggagattct gcgtcagcgc gatatcttca
    21241 ccccacgttg tcgacaactg ttagaggaat atgagcagca gggcggtttt aacgaaacac
    21301 aggcacagga gtttgtgcag gaagccctgg aaacgtttcg ctggcaccag tcagcaacgg
    21361 tagatgaaga aacctatcgc gccttgcaca acgaacatcg gttgattgct gatgtggtct
    21421 gttttcctgg atgccatatc aaccacctga cgccacgtac gctggatatt gaccgggtgc
    21481 agtcgatgat gcctgaatgc ggaattgaac ccaaaattct catcgagggg ccgccgcgcc
    21541 gcgaggtacc gattttacta cgccagacca gctttaaagc actggaagag acggtgttgt
    21601 ttgcggggca gaaacagggc acgcataccg cgcgctttgg tgaaattgag cagcgtggcg
    21661 tggcattaac gccgaaaggg cgacaactgt atgatgatct tctgcgtaac gctggaaccg
    21721 ggcaggataa tctcactcac caaatgcatt tacaggaaac cttccgcact tttcctgaca
    21781 gtgagttttt aatgcgtcag caagggttgg catggttccg gtaccgtctg acgccttcgg
    21841 gtgaggcgca tcgtcaggcg attcatcctg gagacgatcc acagccctta attgaacgtg
    21901 gttgggtagt ggcgcaaccc atcacctatg aagatttctt gcccgttagc gcggcgggga
    21961 tcttccagtc aaatctgggt aatgaaacgc agacacgcag tcacggtaat gccagtcgcg
    22021 aagcatttga gcaggcgttg ggttgtccgg ttttggatga gttccagctt taccaggaag
    22081 cggaagaacg cagtaaacgt cgctgtggtt tgctttaaat tctgaccatc cgcctttgca
    22141 aaaatttgcc tgattttaca aacgaatcag gctcatccca tcgacataaa aaaaatgccg
    22201 atttatgcat attctctcag ttcaacaatt ggattattaa taaatattgt ctagagtgag
    22261 cggtcataaa taagcacttt cttgccgctg aaaacgacca gcgcgggacc attcacaaca
    22321 ccagaaggac tcactttcag gtatggatcg tagacgattt attaaaggtt caatggctat
    22381 ggccgccgtg tgcggtacca gcggcattgc ttctcttttt tctcaggcgg cattcgcggc
    22441 agattctgat attgccgacg ggcaaaccca gcgttttgac ttctccattc tacagtcaat
    22501 ggcgcacgac ttagcgcaaa cagcgtggcg tggtgcgcct cgtccgttac ctgacacgct
    22561 ggcgacaatg acgccgcagg cttataacag tattcaatac gacgccgaaa aatcgctctg
    22621 gcataacgtt gagaaccgtc aactggacgc tcagttcttc catatgggaa tgggattccg
    22681 tcgccgcgtt cgtatgtttt ctgtagatcc agcaacacat ctggcgcgtg aaattcactt
    22741 tcgcccggag ttgttcaaat acaacgatgc gggtgttgat acaaaacaat tagaagggca
    22801 aagcgatctc ggctttgccg gttttcgcgt gtttaaagcc cccgaactgg cgcgccgtga
    22861 tgtagtatca tttctcggcg cgagttattt ccgcgccgtt gatgatacat atcaatacgg
    22921 tttgtcggcc cgcggcctgg cgatcgacac ttacaccgac agtaaagaag agttccccga
    22981 ctttaccgcc ttctggtttg atacggtaaa accgggggca actaccttta ccgtttatgc
    23041 gttgctcgat agcgccagca ttactggtgc ctataagttc actatccatt gtgagaaaag
    23101 tcaggtgatt atggatgtgg aaaatcacct gtatgcgcgc aaagacatta aacagctggg
    23161 cattgcgccg atgaccagta tgttcagctg cggtactaat gaacgtcgga tgtgcgatac
    23221 aattcatccg caaattcatg actctgatcg tctgtccatg tggcggggca acggcgagtg
    23281 gatttgccgt ccgctgaata atccgcaaaa attgcagttc aatgcttaca ccgacaacaa
    23341 cccgaaaggg tttggtttat tgcaactgga tcgtgacttc tcccattatc aggacattat
    23401 gggctggtat aacaaacgcc caagtctgtg ggtggagccg cgtaacaagt ggggtaaggg
    23461 caccatcggc ctgatggaaa tcccaacaac gggcgaaacg ctggataaca ttgtctgctt
    23521 ctggcagcca gaaaaagctg taaaagcagg tgatgagttt gcattccagt atcgtctgta
    23581 ctggagtgcg caaccgcctg ttcattgccc attagcgcgc gttatggcga cgcgtaccgg
    23641 catgggcggt ttcccggaag gttgggcgcc aggtgaacac tatcccgaaa aatgggcgcg
    23701 tcgttttgcc gtcgatttcg ttggtggtga tctgaaagct gccgcgccaa aaggcattga
    23761 gccggtgatt acgctttcca gtggggaagc gaagcaaatc gaaattctct atattgaacc
    23821 catcgatggt tatcgtattc agtttgactg gtatccgact tcggactcca ctgatccggt
    23881 cgatatgcgg atgtatctac gttgtcaggg ggacgctatc agtgaaacat ggctgtatca
    23941 gtatttcccg ccagcgccgg ataaacgtca gtatgttgac gaccgcgtga tgagttaatc
    24001 gttttttctt cggcaccttc ttcgggaggt gccgtctggt taaacacgat cccgctcgca
    24061 tttttcccta agttaaatga gtaatctgat ggtgtgtatt tcagatacac cttgtcagcc
    24121 actaacaggg agtgcgtatg tttccagaat accgagattt aatatcccgt ctgaaaaacg
    24181 aaaatcctcg ctttatgtcc ttgttcgata aacacaataa acttgatcat gaaattgcca
    24241 gaaaggaagg ttccgacggt cgagggtaca atgcggaagt ggtccgcatg aaaaaacaaa
    24301 agctacagtt aaaagatgag atgctcaaaa tcctgcagca ggagagcgtc aaagaggtgt
    24361 aaactttcct aagccgccag ccaggcggct tttttaacaa cagcatggat tgactggaga
    24421 taagatgact gaaacgataa aagtaagcga atcacttgaa ttacatgctg ttgcggaaag
    24481 tcacgtcaca cctctttatc agttaatctg taaaaataaa acctggttac agcagtcgct
    24541 aaactggccg cagttcgttc agagtgaaga ggacacgcga aaaacggtgc agggtaatgt
    24601 gatgttgcat caacgcggct atgccaaaat gttcatgatt ttcaaagaag atgaacttat
    24661 cggcgttatc tcgtttaatc gtattgaacc actgagtaaa actgctgaaa tcggctactg
    24721 gctggacgaa tctcatcagg ggcaggggat catttctcag gcgctgcagg cattgattca
    24781 tcattacgct cagtctggtg aacttagacg cttcgtgatc aaatgtcggg tggacaatcc
    24841 gcaaagcaac caggtcgctt tgcgcaatgg ttttatcctt gaaggttgcc tgaaacaggc
    24901 tgagttcctg aatgatgcct atgatgatgt gaacttatac gcgcgtatta tcgattcata
    24961 ataaccctgc cagcggcgtt cgcgtaatgc gctcctcgcc gttaatgact tttggaccac
    25021 ggacatgaac cgtgtcgcca tcaaaggctt caacgacggc gtggtcggtt aactccacat
    25081 ggttttcaat tatcaccgca cctgaaatac ggctctcacc ttgtattaca acgtgctcgt
    25141 cgagcagaat cggtccacca cgtaccacgg catttccgcc aatcaagaca tgatgtttta
    25201 acacacaatt accttccaca atggcatatt ccgccacctg cgaactgtaa tgaatcgtcg
    25261 gaatggcatc ttcttctatg ccagctttca cctgcgcgtg accgtagact ttagcgcaat
    25321 cgcatagcca gacgttgttc tcttcattac cttcaacact ggcaaaatca aaaacttcgg
    25381 cgcgatgttc aataaaagca taccggacga cggcatcgcc ataaatttgt gcctggtgga
    25441 caatacgcga ggcgctaacc cttgcgcgat cataaatttg gagcaggagt tgatggtcgg
    25501 gcgttaagcc ttgtgcggcg acgatcatag aatgttgatc aattaaggcg tgaccaaata
    25561 ttctacattg cccgcaaacc agtgagtcat gaatggtgac gctgtcactt atatatgcac
    25621 cctggctgat ttcactatta tcgatccaga cattatccgt tgcgtaaacc tctccccaca
    25681 gcacgctggt gccggtgatg cgcgtattac cggaaatcac cgctccgccg aacgcaatag
    25741 cgttctggtc gtaaatccag caatttcctt cctgtgctaa caccgtttcg cgatcgatcc
    25801 agccgccagc ggttcctgct atcacatcgt taaaatcgct aatggcgata atctgccgta
    25861 ataacacatt ttttttagtg ccatcttcct gataactaaa ggctcgctgt tcttcactta
    25921 gacgatattt gcgcatagcg ttttcccaca ggtgacttac tataaccgta gcaaattctg
    25981 cggctctggc tatgctcgag aaaattccat aaaatgcatt tcaaatatac tttataaatt
    26041 aaataaaatg agtaagaaga tgcagagcga taaagtgctc aatttgccgg caggctactt
    26101 tggtattgtg ttggggacga tagggatggg atttgcctgg cgctatgcca gccaggtttg
    26161 gcaggtcagc cactggttag gggatgggct ggtgattctg gcgatgatca tctggggatt
    26221 gttaactagc gcatttatta cccgacttat acgctttccg catagcgtgc tggcagaagt
    26281 gcgccatcca gtgatgagca gttttgtgag tttgtttccg gcaacgacga tgctggtggc
    26341 gattggtttt gttccgtggt ttcgcccact ggcggtgtgc ctgttcagtt ttggtgtcgt
    26401 ggttcagttg gcttatgccg cctggcaaac tgcgggatta tggcgcggat ctcaccctga
    26461 agaagctacc acgcctggac tgtatctgcc gacagttgcc aacaacttta tcagcgcaat
    26521 ggcctgtggt gcgttgggct acaccgacgc cggtctggtg tttttaggcg caggcgtttt
    26581 ctcatggcta agccttgaac ctgtgatctt gcagcgtctg cgcagttcgg gagaattacc
    26641 cacggcactg cggacatcac tcggcattca gctcgctcct gcgctggtgg cctgtagtgc
    26701 ctggttgagc gtcaacggcg gcgagggtga tacgctggcg aaaatgcttt ttggttatgg
    26761 actgctgcaa ctgctgttta tgctacgtct gatgccatgg tatctctccc agccatttaa
    26821 tgcttcattc tggagtttct cgttcggcgt atctgcactg gcaaccaccg gtttgcatct
    26881 ggggagtggc agcgaaaatg gatttttcca tacgctggcg gtaccgctgt ttatctttac
    26941 caattttatt attgcaatac tgctcatccg tacttttgcg cttctgatgc agggaaaatt
    27001 gttagtcaga accgagcgcg ccgttttaat gaaagcagag gacaaagaat gatcattcgt
    27061 gacgaaaact attttactga taaatatgaa ttaacccgca cacactctga agtactggaa
    27121 gcggtgaaag tggttaaacc gggtaaaacg ctggatctgg gctgtggcaa tggtcgtaac
    27181 agtctttacc tggcagccaa tggttatgat gttgacgcat gggataaaaa tgccatgagt
    27241 atcgccaatg tagagcgcat taaatccatt gaaaatctgg ataatttaca cacccgagtc
    27301 gttgatctga ataacctcac atttgataga cagtacgatt ttattctttc gactgtggta
    27361 ctgatgttcc ttgaggctaa aaccatccca gggctgattg ccaatatgca acgttgcact
    27421 aaacctggtg gttacaacct gattgtggcg gcgatggata ccgctgatta tccatgtacc
    27481 gtcggcttcc cgtttgcctt caaagaggga gaattacgtc gatattacga aggctgggag
    27541 agggtgaaat acaatgaaga cgtcggcgag ctgcaccgca ccgacgccaa cggtaatcgt
    27601 attaaactgc gtttcgccac gatgctggca cgtaaaaaat gacccggtaa gcacaaaacg
    27661 cgtgaaaatt ccccacgctg agatgattta ctgttcttct tttcggtaag catatttttt
    27721 atcgaaggga tgtgaaatta atcacagtag tcgaagtttt tagcagctta acttactgaa
    27781 atttaactac tgatgattga cttagcccct ttttcggcat tgactatgtc gtctgaaaag
    27841 gggctgaaaa atttattttc accaacactt tttttgccac aacacgaagc ggcgcttttt
    27901 gctataactt agaaagtaat ataatcatct caggaaacta ttcatgcgta ccacatcatt
    27961 tgcgaaagtt gcagctttat gcggcttgtt ggctctgtct ggttgtgcct ctaaaatcac
    28021 ccagccagat aaatattctg gttttttaaa caattactct gatttaaaag aaacaacctc
    28081 agcaacaggt aaacctgttt tacgttgggt agacccgagt tttgatcaaa gcaaatatga
    28141 cagcatcgtc tggaacccaa tcacttatta tccggtacca aaaccttcta cccaggtagg
    28201 gcagaaagtt ctggataaaa ttttgaacta taccaacacc gaaatgaaag aagcgatagc
    28261 gcagcgtaaa ccagtggtta ccaccgctgg gccgcgtagt ctgattttcc gtggggccat
    28321 taccggtgta gataccagca aagaagggct gcaattctat gaagtggttc ctgttgcatt
    28381 agtggttgcg gggacgcaaa tggctacagg ccaccgtacc atggatactc gcctctattt
    28441 tgaaggtgag ctgattgatg cagcgactaa taaaccggtt atcaaagtcg ttcgtcaggg
    28501 cgaaggtaaa gacctgaata acgaaagtac gccaatggct ttcgaaaata ttaaacaagt
    28561 tattgatgac atggcgaccg atgccaccat gtttgacgtt aacaaaaagt agttccagac
    28621 gcgccatcgt tagatggcgc tttttatccg gtgcgccgta aaaccccatc cttcagggcg
    28681 gggatataag gcgcggtttt ccacctgacc aggtgtttgc tgttgttcaa tgtattggcg
    28741 gatgacggat attggcgcac ctccgcagct actggcaaaa tagccag
//
LOCUS       DAZKIV010000045        28551 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZKIV010000045 DAZKIV010000000
VERSION     DAZKIV010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28551
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.045"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            58..720
                     /gene="acrS"
                     /locus_tag="ACHOT6_001966"
     CDS             58..720
                     /gene="acrS"
                     /locus_tag="ACHOT6_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFP9947190.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQDHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(723..902)
                     /locus_tag="ACHOT6_001967"
     CDS             complement(723..902)
                     /locus_tag="ACHOT6_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFP9947191.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(986..1870)
                     /gene="yhdJ"
                     /locus_tag="ACHOT6_001968"
     CDS             complement(986..1870)
                     /gene="yhdJ"
                     /locus_tag="ACHOT6_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFP9947192.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NRSKRCRVSEVDPDLIAK"
     gene            complement(1956..2252)
                     /gene="fis"
                     /locus_tag="ACHOT6_001969"
     CDS             complement(1956..2252)
                     /gene="fis"
                     /locus_tag="ACHOT6_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFP9947193.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(2278..3242)
                     /gene="dusB"
                     /locus_tag="ACHOT6_001970"
                     /pseudo
     CDS             complement(2278..3242)
                     /gene="dusB"
                     /locus_tag="ACHOT6_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
     gene            complement(3571..4452)
                     /gene="prmA"
                     /locus_tag="ACHOT6_001971"
     CDS             complement(3571..4452)
                     /gene="prmA"
                     /locus_tag="ACHOT6_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFP9947194.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(4464..5915)
                     /gene="panF"
                     /locus_tag="ACHOT6_001972"
     CDS             complement(4464..5915)
                     /gene="panF"
                     /locus_tag="ACHOT6_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFP9947195.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5905..6147)
                     /locus_tag="ACHOT6_001973"
     CDS             complement(5905..6147)
                     /locus_tag="ACHOT6_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFP9947196.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(6256..7605)
                     /gene="accC"
                     /locus_tag="ACHOT6_001974"
     CDS             complement(6256..7605)
                     /gene="accC"
                     /locus_tag="ACHOT6_001974"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFP9947197.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(7616..8086)
                     /gene="accB"
                     /locus_tag="ACHOT6_001975"
     CDS             complement(7616..8086)
                     /gene="accB"
                     /locus_tag="ACHOT6_001975"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFP9947198.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8952..9782)
                     /locus_tag="ACHOT6_001976"
     CDS             complement(8952..9782)
                     /locus_tag="ACHOT6_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947199.1"
                     /translation="MTSLFNRLTGKAVSRTAFVEHLGQEVVQHHPNWKVMISTDHKLM
                     RIDTGHQTVEITLDDAYKRYQAAPDPLQEVAADMLKQLAEMLPTGRCIVPVIKEKAWL
                     DTVRQQLSQYESDPEVLNASLDFAWEDFNDELIILFAEDGSETMRYLMRYEVTIDREQ
                     AITNLKQILPEVTAEIVDTGANDGKVAVISAGGTYEPSLMLIDDLWVQSPDPECGDLL
                     VTVPSRGKLFFTHNDQPANLSALRQLTEQCFREDDHPLTTQIFIRRNGHFYPWVETLH
                     "
     gene            complement(9819..10793)
                     /locus_tag="ACHOT6_001977"
     CDS             complement(9819..10793)
                     /locus_tag="ACHOT6_001977"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFP9947200.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            10945..12885
                     /gene="csrD"
                     /locus_tag="ACHOT6_001978"
     CDS             10945..12885
                     /gene="csrD"
                     /locus_tag="ACHOT6_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFP9947201.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNVGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13190..14233
                     /gene="mreB"
                     /locus_tag="ACHOT6_001979"
     CDS             13190..14233
                     /gene="mreB"
                     /locus_tag="ACHOT6_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFP9947202.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            14299..15402
                     /gene="mreC"
                     /locus_tag="ACHOT6_001980"
     CDS             14299..15402
                     /gene="mreC"
                     /locus_tag="ACHOT6_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFP9947203.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQSPARAPGGQ"
     gene            15402..15890
                     /gene="mreD"
                     /locus_tag="ACHOT6_001981"
     CDS             15402..15890
                     /gene="mreD"
                     /locus_tag="ACHOT6_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFP9947204.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            15899..16492
                     /gene="yhdE"
                     /locus_tag="ACHOT6_001982"
     CDS             15899..16492
                     /gene="yhdE"
                     /locus_tag="ACHOT6_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFP9947205.1"
                     /translation="MTSLYLASGSPRRQELLAQLVVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVATDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            16482..17951
                     /gene="rng"
                     /locus_tag="ACHOT6_001983"
     CDS             16482..17951
                     /gene="rng"
                     /locus_tag="ACHOT6_001983"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFP9947206.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            18019..21819
                     /gene="yhdP"
                     /locus_tag="ACHOT6_001984"
     CDS             18019..21819
                     /gene="yhdP"
                     /locus_tag="ACHOT6_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFP9947207.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDRLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGLHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIELPYHAGATYNVELNGDLKNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTQVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            21975..23420
                     /gene="tldD"
                     /locus_tag="ACHOT6_001985"
     CDS             21975..23420
                     /gene="tldD"
                     /locus_tag="ACHOT6_001985"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFP9947208.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(23548..24477)
                     /gene="aaeR"
                     /locus_tag="ACHOT6_001986"
     CDS             complement(23548..24477)
                     /gene="aaeR"
                     /locus_tag="ACHOT6_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFP9947209.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            24660..24863
                     /gene="aaeX"
                     /locus_tag="ACHOT6_001987"
     CDS             24660..24863
                     /gene="aaeX"
                     /locus_tag="ACHOT6_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFP9947210.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            24871..25803
                     /gene="aaeA"
                     /locus_tag="ACHOT6_001988"
     CDS             24871..25803
                     /gene="aaeA"
                     /locus_tag="ACHOT6_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFP9947211.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            25809..27776
                     /gene="aaeB"
                     /locus_tag="ACHOT6_001989"
     CDS             25809..27776
                     /gene="aaeB"
                     /locus_tag="ACHOT6_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFP9947212.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFNT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            27868..28140
                     /locus_tag="ACHOT6_001990"
     CDS             27868..28140
                     /locus_tag="ACHOT6_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFP9947213.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(28196..28459)
                     /gene="yhcN"
                     /locus_tag="ACHOT6_001991"
     CDS             complement(28196..28459)
                     /gene="yhcN"
                     /locus_tag="ACHOT6_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFP9947214.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         6839 a         7140 c         7578 g         6994 t
ORIGIN      
        1 aaccttcgct attttctcac tctgtgtcga atatatttat ttcctgaata attaatcatg
       61 gcaaaaagaa ccaaagccga agctctgaag acccggcaag aactgattga aactgccatc
      121 gcccagtttg cgcagcatgg cgtaagcaag acgacactca acgacattgc cgacgctgct
      181 aacgttacgc gtggcgctat ctactggcac ttcgaaaaca agactcaact gtttaatgag
      241 atgtggttgc aacagccttc attgcgagag ttaatccagg atcacttgac ggctggatta
      301 gagcatgacc cgtttcaaca attgcgtgaa aaattgattg tcggcttgca atatattgcc
      361 aaaattcccc gccagcaggc gttgctgaaa atcttatatc acaaatgtga atttaatgat
      421 gagatgctgg ccgagggagt gatacgcgaa aagatgggct tcaatccgca gactctccgc
      481 gaagtattgc aggcgtgtca gcaacaaggt tgtgtagcaa ataacctcga tttagatgtt
      541 gtgatgatta ttattaatgg tgccttcagc ggaattgttc aaaactggtt aatgaatatg
      601 gcgggttatg atctttataa acaagccccc gctctggtcg ataacgtatt aagaatgttc
      661 atgccagatg aaaacataac gaaattaatt catcaaacga atgaattaag tgtcatgtaa
      721 attcacattc tgtcctgaaa attcagttcg tcacagttaa ccagttttag tggattaact
      781 gagttcatat tgcggcattt gtctgtttct gttgccagca gttcaatcca ctgcatcagt
      841 aaagtatcaa acagaaaaac gaagacagcg aaaacgacga gccaccaata cttgcgaatc
      901 attacctgag cctgtgtatg tttaaccaag tctggcagga aatatacacg aaatgaataa
      961 tctgaaaacc aggcttacac ttcctttact ttgcaatgag atcggggtca acttcgctaa
     1021 cccgacatcg ttttgaccgg ttgcccgtct ttcttttttt cactttcgcc agttcctccg
     1081 cggagtaatg tgacgcgaca tccagtcgtc gaagccccat tttgatgtac tcgctgttga
     1141 tctcaatacc aatgaatttt cgcccgctgg cgacggctac ggcaccggta gtaaagctac
     1201 cggcaaacgg gtcgagaacg atatcgcctg ggttggaaga ggcgagaata atgcgtttca
     1261 gtaaggcttc cggtttttgc gtcgggtggt tctcatattc atccatcaaa taacgcacgc
     1321 gcggaaaatc ccagacgtta cccggtactt tttgatgatt gtatggctgt ggaggatttt
     1381 tgcgataatc gatcaacgcg cgctgcgatc cggttttggc ttctaccaga atagcatcac
     1441 cgttgaatgt gtagttcttt gcgtctttca ccatcatcag gatgggttcg tacatggagc
     1501 cgtagtgttt tttcgcctgt actccagaac tgtcatatga ccagacgatg cgacttttga
     1561 tggtaaaaag cttgcggcac tggagatcga taaagggcat gttttccgta ctgttcataa
     1621 tgtacatgct gccctgcttt ttcagaacgc ggtggcactc tgcaatcact tcaaacagcc
     1681 agtcgataaa cagatcttct ttccaggctt cgatcagacc atcaaaattt ttaccgatgt
     1741 tatacggtgg gtcggcaaag atcagatcga cgctttcagt aggtagcttt ttaagttcgg
     1801 caagggcatc accgtgaata atggtcttag cttcattacc aaaccgggtc ggttcacatc
     1861 ctgttctcat ggtcactccc tttgtgacac ctataaaaaa ggcgcttccc catgccgagt
     1921 agcgcctttt taatcaagca tttagctaac ctgaattagt tcatgccgta ttttttcaat
     1981 tttttacgca gcgtaccacg gttgatgccc atcatcagcg cagcacgggt ctggttacca
     2041 cgggtgtatt gcatcaccat gtccaacagg ggctgttcta cttcagccag taccagctca
     2101 tagaggtcat tcacatcctg accattcagt tgagcaaaat agttcttcag tgcctgttta
     2161 accgagtcac gcaggggttt ttgggttacc tgatcctgag agttaacggt agaaacggtc
     2221 agtacgtcag aatttacgcg ttgttcgaac atagttctgt cagctcttta tttctgttta
     2281 cgcaaaattt tcgaagtatg cctccaacgc ctccagctgt tcgctggcat cctcaatggc
     2341 gttgaatgtg cgccgaaact ggtcatttgg agcgtgttcc tggagatacc aggaaacgtg
     2401 tttacgtgca attcggtacc cttttgccgg accataaaag tcatgcagtt cccgaacgtg
     2461 cgcgcaaagc aagcgcttaa cctctgccaa aggcagcggg ggcagcaact ccccagtgtc
     2521 cagataatgc tggatttccc gaaagatcca gggtcttccc tgagctgcgc ggcctatcat
     2581 cagggcatcc gcccctgtat agtcgagcac agctctggct ttaagcgggt cagtaatgtc
     2641 gccattcgcg ataaccggaa tggaaacttt ctgcttaact gcccgaatac tgtcgtactc
     2701 agcttctcca ttgaacaaac aggcgcgtgt acggccatga atggtcagag cctgaatgcc
     2761 acagtcttca gccagttggg caatctcttc gcagttacgg tgttccggtg cccagccggt
     2821 gcgaatcttc agggtaacag gaacgtccac tgcattgacg acctcggtaa ggatcgattt
     2881 aacgacatcc gggtactgca agagggctga acctgcgagc ttgcgattca cttttttagc
     2941 tgggcaaccc atattgatat caataatctg ggcaccgctt tccacgttaa tacgtctgca
     3001 tctgccattt ctttcggatc gctaccagca atttgcacgg tgcgaatacc gggttcatca
     3061 atgtgcacca tccgtaaacg agatttgtcg ctttcccaaa cctgtgggtt agaagacatc
     3121 atctcggata ctgtcaatcc ggctcccatc tcgtagcaca acgtccgaaa aggtctgtct
     3181 gtaatgccag ccatgggcgc tgcgatcagg cgatttctga gctgatattg tccgatgcgc
     3241 atgagttaag aaatgaccat actgtgactg caaggcggcg tatattacgc attttttgca
     3301 cgagatgaaa ggccaaactt tgaccaatcc tctgagatgg atcaaagaat tgcatttaaa
     3361 atgagcgtgg tgcgataatt actcataaaa atcatcatat tagaaaatag tgactaaaat
     3421 ttacactcaa agaaatttga gtaagttctc aatttttctt tatgaatgaa aattttggca
     3481 cgcaaatttg cgtaaataac cggcaaattt acgtgccttt tgtgagcttg ctcgcacttc
     3541 gccccgcgtc accctacggc gatgcgaagg ttaattctta cgaccggtaa tacggcacca
     3601 ctcttctttt tccacgaccg ggtccagtgc gaagctatcg gcataagctt cacaaacgct
     3661 ctctgcctgg cttgccagaa taccggaaag gcccagcaaa ccgcctgaaa ccggcaggac
     3721 gctgattaac ggtgccagtt cacgtaatgg gcctgcaagg atgttagcga ccaccacgtc
     3781 ggctttcatt tcttctggct gatctttcgg taagtagagt tccagacggt cagaaacgcc
     3841 attacgttcg gcgttgtcgc ggctggcctg aatcgcctgc ggatcgatat caataccaat
     3901 ggcttttgct gcacccagtt tcagcgccgc gatcgccaga atgccggaac cacagccaaa
     3961 gtcgatgact gttttaccgg ttaaatcgag gctgtcgagc cattgcaggc acagagaggt
     4021 ggttggatgg gtacccgtac caaacgccag ccctggatct aacatcacgt tgacggcgtt
     4081 ttcgtccggc acatcacgcc agctagggca gatccacagt cgttcaccaa agcgcatcgg
     4141 gtggaaatta tccatccatt cgcgctccca gtctttatct tctagttgtt cgattttatg
     4201 cgcgaagcct gcgccgagca gcggatggtt ttccagaatc gccaccacgt cgttcatatc
     4261 ggtttcagcg tcgaacagac caatcacatc ggtgtcgccc cacaggcgcg tttcgcccgg
     4321 cagcggctca aatactggcg tatcgtgggt atcctgaaaa gtgatagaaa cggcacccgc
     4381 ttccatcagc gcatcgctaa gatcttccgc gttcgcgccg gtggtgttca gtttcagttg
     4441 gatccaaggc atggcaaaac tctttattta tcagtagtca aaacggtagc ttgcgggacg
     4501 gatgtaccga aacggtttcc gaccaggaaa gccagcaaac ttagtagtaa cgagggcacg
     4561 atagggtgga agcccaggta ctgaatattc agcgtcgcga gtacggcata cagcacgccg
     4621 ccaacgatca tcgcacttag cgcgcctttg gcgttggcgc gttcccagta aagacccagc
     4681 accagcggcc acaggaaaac ggcttccagc ccaccgaagg ccagcaaatt cagccagatg
     4741 atcatttctg gcggcttcca ggcggcaagc agcagcaacg cgccgagaac taacgtaatt
     4801 accgccgaca tccgcttcag acgcgtctcg ttttgcattt gatccggacg gatattcaga
     4861 tagagatctt taatgatcgt agcggaactt tgcagcaact gggcattgat cgtcgacatg
     4921 atcgcagcca taggtgcagc caggaagatt ccggcagcaa acggtggcag cacttttacc
     4981 attaacgtcg ggatcaccag atccggtacg gtgagatcgg ggatcactgc acgacctaac
     5041 gctccggcca ggtgcatacc gaacatcagg attgcgacta caatcgtacc gatgatgatc
     5101 ccccggtgta ctgctttgct gtctttataa gagatacagc gcaccgcagt atgcggcagg
     5161 ccaatcacgc caaagcacac cagtacccag aacgacgtca taaaggcagg cgacagaata
     5221 tcgtcagcgc cttgtggcgt aaccagttgc ggatcgatgg tttgcaaggt ctgtactgcg
     5281 ttgcttaagc cgccagcggc atgtaccacg ccaataagca gcacaacggt gccaatcagc
     5341 atcacaagcc cttgcatggt gtcgttcagc acgctggcgc gaaagccacc aaaggcggta
     5401 tataacgcaa tgctgatacc aaaaatcagc agcccggttt cataaggaat acccgccgcg
     5461 gtttccagca ggcgcgcacc gccgataaac tgcacggtca tcgcaccaac gaacgcaacc
     5521 agcaaactca aactcgccag ccacaccaga agacgactct ggtagcgggc aaacagcata
     5581 tcgttcagcg tcactgcatt gtagcggcgc gcaagaatcg caaacttctt gccgagaata
     5641 ccgagtgaaa gccagactgc aggaagctga atcatcgcca gcaataccca gcctaggccg
     5701 tatttataag cagctcctgg cccgccgata aacgaactgg cactgatata ggtcgcggtg
     5761 agcgtcatcg ccagcacaat accgcccata gagcggctgc cgaggaaata ctcattaagg
     5821 aaggtgccgg tgctccgttt acgcatcgca taaacagaga taccgaacac caccaccaga
     5881 taggcgacca gcggtagaat tacttcaagc tgcatcgtca tcctccagtg ggatatcgcg
     5941 atagataaat ttcaccatcg cccagcacag tccaataaac agcagcggcg tcaggatgca
     6001 ggccatctca aaccagcgcg gaaagccggt aaaaccgggg gcaacgccag gtaagtaagc
     6061 ggctactaac cataccgcca gatacaaaag ggtcagcccc agcgcccagc gcgcctcttt
     6121 atgggcctga acaaaacgag tgtccatttt ttgtccctga tgggtgaaga aagcgaggat
     6181 tgtaccttat gggagttgtc gatccccagt aataaaaaag gccggaaaat ccggcctttt
     6241 gacgcttttg cagtcttatt tttcctgaag accgagtttt ttctccagat agtggatgtt
     6301 agtgccacca tgctggaagt tctcgtcatt catgatgcgg atctgcagat caacgttggt
     6361 tttgataccg tcgatgatca gctcctgcag cgcattcttc atgcgggcaa tcgccacgtc
     6421 acggttttca ccgtagcaaa tcagcttacc gatcattgag tcatagtacg gcggtacggt
     6481 gtagcccgcg tagatatgag actcccaacg tacgccaaaa ccgccaggtg cgtggaaacg
     6541 ggtgattttg cctgggcttg gcaggaaggt gttcggatct tcggcgttga tacgacattc
     6601 caccgcatgg ccgcgaacgt gaacttcttc ttgcttgatc gacagcggtt gaccggcagc
     6661 gatacgcagc tgttctttga tcaggtcaac gccggtgatc atttctgtaa ccgggtgttc
     6721 tacctgaata cgggtgttca tttcgatgaa atagaactcg ccgttttcga acaggaactc
     6781 gaaagtacct gcaccgcgat agccgatatc aacacacgct ttagcgcaac gttcgccgat
     6841 gtagcgacgc agttccgggg taatgcccgg tgctggtgct tcttcgacca ctttctggtg
     6901 gcggcgctgc atggagcagt cacgttccgc cagatagata gcgttgccct gaccgtcagc
     6961 cagtacctga atctcgacgt ggcgaggatt ttccaggtat ttctccatgt aaaccatatc
     7021 gttgctgaaa gcagctttcg cttccgcacg ggtcatggag atggattgtg ccagttcagc
     7081 gtcgccgcgc actacgcgca taccgcgacc accgccgccg ccggaggctt tgataatcac
     7141 cggataacca atgcgtttag caatggcacg gtttttatcc atatcgtcgc ccaacgggcc
     7201 gtcagaaccc ggtacgcaag ggacgcccgc ttttttcatc gccgcgattg cggatacttt
     7261 gtcgcccatc aggcgaatgg tttctgcttt cgggccgatg aagataaagc cggagcgttc
     7321 aacctgctcg gcaaagttgg cgttctcgga gaggaagccg taacccggat ggattgctac
     7381 tgcgccggtg atttcagcgg cgctgatgat tgccgggatg ttcagataac tttttactga
     7441 cggagcaggg ccaatacaga ccgtttcatc tgccagtaat acgtgtttta gatcgcgatc
     7501 cgcgctggag tgcacagcga cagtcttgat gcccagttct ttacaggcac gaagaatacg
     7561 caatgcaatc tcgccgcggt tggcaataac aattttatcc agcatgttcg cctcgttact
     7621 cgatgacgac cagcggctcg tcaaattcta ccggttgtcc actttcgacc agaattgctt
     7681 tcacggtacc ggatttgtcc gcttcgatct ggttcatcat tttcatggct tcaacgatgc
     7741 acagggtatc gcccacgttg actttctgac ccacttcgat gaacgctttt gcgtccgggc
     7801 ttggggtgcg gtagaaagta ccaaccatcg gggaacgtac gatgtgacca ctgatttccg
     7861 ctgctgctgg cgcttccatg gaaggaacgg tcgccggagc ggctgcgtta gattgagctg
     7921 gctgctgcat cattggtgca gcgtaagctt gttgcatcac agggaaactt gcggcaggag
     7981 ctgcacggct aatgcgtact gactcttcgc cttcagaaat ttccagttcg gagatgcctg
     8041 attcttcaac cagctcgatc agttttttaa tcttacgaat atccatgagt gggttccgta
     8101 ctctttgttt agtgtgattg tgacaggcgt ttcaccgccg tctgtaaagc gtatgccccg
     8161 acagctgtat gcatagcgat aaattccagc aggccgggac gccggtctat tttgccccag
     8221 agtgtcaatg ttagacttga cggacattgt gcagcgatct gctgctatcc tccggcaaaa
     8281 aacaaaatat accttcgttg cgccaatgtc accttttccg cagcgcaaaa actgcgtcag
     8341 ggaggacgag gtcgcacatt ataacgattt cgtagcaatt ggcagctaaa tactggtctt
     8401 atcagggaag ataatcaaca gctaacatgt aaataacctt caacaccgtg taatttgcaa
     8461 caagccgcac aattcacgaa attagcgcca tcatcgacgg aacttcttat aacgtaaggt
     8521 gaaaagctga aaagccagcc ctgcgaagat gaggggctgc aatgagataa tcttcacaga
     8581 ccacaaataa tgtagggatg ccaggatcgc gacaagatag acgacattac gcagcggttg
     8641 ctggggatta cccaattttc actgcatcgt ctggatagat gtgaatgcta aagcacgcaa
     8701 aatcaaccga ctgacaattc tcagcattaa ataggttcga gtggttaatt tgtagtcaaa
     8761 aaaaatcaga ttattcaccc cattttgcag aaggtaaact gttttgtcag gcggcagtgc
     8821 ggctatctga tgctttcaga atttgccacc tgacaataaa aaagccgatt tggtagcaaa
     8881 atcggcttcg gtgagtttgc agcattcatc acatactccg acaagccttt tcaagcatga
     8941 cggaggttgc gttaatgcaa ggtttctacc cagggataaa agtgaccgtt gcggcggatg
     9001 aatatctgtg ttgtcagagg atggtcatcc tcacggaagc attgttctgt tagctgtcgt
     9061 aatgcgctga gatttgctgg ctgatcattg tgggtgaaaa acagttttcc tctggaggga
     9121 acggtcacta acaggtcgcc gcattcagga tcgggtgatt gcacccaaag atcgtcaatg
     9181 agcatcagac tgggctcata agtaccccct gcggagataa cggcaacttt tccatcgttt
     9241 gcgcctgtat caacgatttc agccgtaact tccggcaata tttgcttcaa attagtgatg
     9301 gcttgttcgc ggtcaatggt cacttcgtag cgcatcagat agcgcatggt ttcgctacca
     9361 tcttcagcaa acaagataat taactcatcg ttaaagtctt cccaggcaaa atccagcgag
     9421 gcgttgagga cctctggatc agactcatac tggctaagct gttggcgaac ggtatccagc
     9481 caggcttttt ctttaatcac cggaacaata cagcgcccgg tcggcagcat ttctgcaagc
     9541 tgtttcagca tatctgcagc cacttcttgc agcgggtctg gtgcggcttg ataacgtttg
     9601 taggcatcat caagggtgat ttccaccgtt tggtgacccg tatcaatgcg catcaatttg
     9661 tggtcagtcg aaatcatgac tttccagttt ggatgatgtt gtacaacttc ctgaccaagg
     9721 tgttcgacga aagctgtccg gctaaccgct tttcccgtta accgattgaa taatgatgtc
     9781 aaaatgagtt ccctctctta ttattccttg ctaaatggtt agttaacctt caccagcgtg
     9841 cgaccctgga tctggttatt aatgatggcc tcggcaaagt tcggtgcctc tgacagagat
     9901 atctcttttg ccgcctgggt atagaatgat tccggtaaat cggcgaccag tcgctgccag
     9961 gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca ccccttgcaa acggacatta
    10021 cgcagaataa atggcatgac cgtggttggc agagtaaaac cacccgccag accacaggcc
    10081 gccacgcagc cgccgtaatt catttgcgcc agcacttttg ccagcacttt gtcgccaaca
    10141 gtgtcaattg ccccagccca gacttgtttt tccagaggac gggattcggc aaactcatca
    10201 cgagggagaa tacggctggc acctaaactt ttcagatatt catgggtact ttcgcgaccg
    10261 gaaacggcaa cgacctgata acccaactta tgcagcagcg ccacggcggt actgccgacg
    10321 ccaccactgg cacccgtcac gacaatctcc ccgtcctgcg ggcgaacacc ggcatcttcc
    10381 agcgccatca cacacagcat ggcggtaaaa ccggcagtac cgataatcat tgctttacgc
    10441 gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt tcactcgcgc ctgttccgcc
    10501 agcccacccc agtggttttc accaacgccc cagccagtga gtaacacctc ctgaccggca
    10561 tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa aatcgatccc aggaatcatc
    10621 ggaaaattac ggatgatttt tcccttaccg gtaatcgcca gcgcatcttt gtagttcagg
    10681 ctcgaccagt gaacatcaac cgtgacatcg ccctccggca ggcgactttc gtccagagtc
    10741 tgtaccgatg cgagagtttt gccgtcctgc tgttctaaaa gtaacgcctg cataagtggt
    10801 cctcatgtgc atgatggatt ggaaaatcat ttctgaagac tatactcgct aatggaaaag
    10861 caaagccgat gaagcgcaat aaattgcgag ataattctga tttgctagta tgcccgcttc
    10921 ctcactatcg gagttaacac aaggatgaga ttaacgacga aattttcggc ctttgttacg
    10981 ctgctcaccg ggttaacaat ttttgtgact ttgctgggct gttcgctaag tttctacaac
    11041 gccattcagt ataagtttag tcatcgcgtt caggcggtgg cgacggcgat tgatacccac
    11101 ctagtgtcga atgacttcag cacattaagg ccacaaatta ccgaattaat gatgtcggca
    11161 gatatcgttc gtgtagacct gctgcatggt gataaacagg tttataccct ggccagaaat
    11221 ggtagttatc gtccggttgg caccagcgat ctgtttcgtg aactgagcgt tccgttgata
    11281 aagcatccgg ggatgtcgct gcgtctggtt tatcaggatc cgatgggcaa ctatttccat
    11341 tcgttgatga ccaccgcgcc gctcacgggg gcgattagct ttatcattgt tatgctcttc
    11401 ctggcggtac gctggttaca acggcaactt gccgggcaag aattgctgga aacccgggct
    11461 actcgtatct taaacggtga gcgtggctct aatgtgttgg gaaccatcta tgaatggccg
    11521 cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa ttcagaacgc acgcgaacaa
    11581 cacagccgtc ttgatacgct gatccgctct tatgccgccc aggacgtgaa aaccggcctc
    11641 aataaccgac tctttttcga taatcagtta gcaacgttac tggaagatca ggagaaagta
    11701 ggtacccacg ggatcgtgat gatgattcgt ctgccggatt tcaatatgtt gagcgatact
    11761 tgggggcaca gccaggttga agaacagttc ttcactctga cgaatctgct gtcgacattt
    11821 atgatgcgct accctggcgc actgctggcg cgttaccacc gcagtgattt tgctgcgctg
    11881 ttaccgcacc ggacgttaaa agaggcagag agcatcgccg gtcagttaat caaagccgtc
    11941 gataccttgc cgaacaataa aatgctcgat cgcgacgata tgatccacat tggtatctgc
    12001 gcctggcgta gtggtcagga taccgagcag gtaatggaac atgcagagtc tgccacgcgt
    12061 aatgtgggat tgcagggcgg caatagctgg gctatttacg atgactcgtt gcctgaaaaa
    12121 ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa tgctcagtcg cggcggcccg
    12181 cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg ttcatcatcg cgaactcatg
    12241 tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg agtatatgcc gatggtcttg
    12301 cagtttggct tatcggaaga gtatgaccgt ctgcaaatca gccgtcttat tccactattg
    12361 cgttactggc cagaggaaaa tctggcgatt caggttaccg ttgagtcgct gattcgcccg
    12421 cgttttcagc gttggctgcg cgatacgtta atgcaatgtg aaaaatcaca acgaaaacgc
    12481 ataattattg aacttgcaga ggccgatgta ggtcaacata tcagtcgttt acaacctgtt
    12541 attcgtttag tgaatgcttt aggggtacgg gtagccgtca accaggctgg tttgacgctg
    12601 gtaagcacca gttggatcaa agaacttaat gttgagttac tcaagctcca tccggggctg
    12661 gtcagaaaca ttgagaagcg aacggagaac cagctgctgg ttcaaagcct ggtggaagcc
    12721 tgctccggga ccagcaccca agtttacgcc accggtgtgc gttcgcgaag cgagtggcag
    12781 accctgattc agcgcggtgt tacaggcggg caaggggatt ttttcgcgtc ctcacagcca
    12841 cttgatacta acgtgaaaaa atattcacaa agatactcgg tttaacctgc cgtttaatct
    12901 gttttcacgt agaataacgc gcgctgcgtc tcatgggagt gtgcttgtct gctcgccaga
    12961 ttgttgcagc acatatgcag atgaatgacc ttacgcggtt gcaaacaggc gaggaatgct
    13021 gctgatgcat taagcctttc tggactcagg cagagatttg taacaaagga aacgaactgc
    13081 actaattttc accgtagcag atgatttttg cgccttgtcg ctgctgcgtg tggttggtaa
    13141 agtaagcgga ttttcttttc cgccccagct ttcaggatta tcccttagta tgttgaaaaa
    13201 atttcgtggc atgttttcca atgacttgtc cattgacctg ggtactgcga ataccctcat
    13261 ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc gtggtggcca ttcgtcagga
    13321 tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat gacgcgaagc agatgctggg
    13381 ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa gacggcgtta tcgccgactt
    13441 cttcgtgact gaaaaaatgc tccagcactt catcaaacaa gtgcacagca acagctttat
    13501 gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc gcgacccagg ttgaacgccg
    13561 cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa gtcttcctga ttgaagaacc
    13621 gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa gcgaccggtt ctatggtggt
    13681 tgatatcggt ggtggtacca ctgaagttgc tgttatctcc ttgaacggcg tggtttactc
    13741 ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct atcatcaact atgtgcgtcg
    13801 taattacggt tctctgatcg gtgaagccac cgcagaacgt atcaagcacg aaatcggttc
    13861 ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt ggccgtaacc tggcagaagg
    13921 tgttccacgc ggttttaccc tgaactccaa cgaaatcctc gaagcactgc aggaaccgct
    13981 gaccggtatt gtgagcgcgg taatggttgc actggaacag tgcccgccgg aactggcttc
    14041 cgacatctcc gagcgcggca tggtgctcac cggtggtggc gcactgctgc gtaaccttga
    14101 ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt gctgaagacc cgctgacctg
    14161 tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac atgcacggcg gcgacctgtt
    14221 cagcgaagag taatcggatg caggcagggg aaatgtctgt ttaccctgcc tggtctgata
    14281 cgagaatacg cataacttat gaagccaatt tttagccgtg gcccgtcgct acagattcgc
    14341 cttattctgg cggtgctggt ggcgctcggc attattattg ccgacagccg cctggggacg
    14401 ttcagtcaaa tccgtactta tatggatacc gccgtcagtc ctttctactt tgtttccaat
    14461 gctcctcgtg aattgctgga tggcgtatcg cagacgctgg cctcgcgtga ccaactagaa
    14521 cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa acagtgaact gctgatgctt
    14581 ggacaataca aacaggagaa tgcgcgtctg cgcgagctgc tgggttcccc gctgcgtcag
    14641 gatgagcaga aaatggtgac tcaggttatc tccacggtta acgatcctta tagcgatcaa
    14701 gttgttatcg ataaaggtag cgttaatggc gtttatgaag gccagccggt catcagcgac
    14761 aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga ccagtcgcgt gctgctgatt
    14821 tgtgatgcga cccacgcgct gccaatccag gtgctgcgca acgatatccg cgtaattgca
    14881 gccggtaacg gttgtacgga tgatttgcag cttgagcatc taccagcgaa tacggatatt
    14941 cgtgttggtg atgtgctggt gacttccggt ctgggcggtc gtttcccgga aggctatccg
    15001 gtcgcggttg tctcttccgt aaaactcgat acccagcgcg cttatactgt gattcaggcg
    15061 cgtccgacag cagggctgca acgtttgcgt tatctgctgc tgctgtgggg ggcagatcgt
    15121 aacggcgcta acccgatgac gccggaagag gtgcatcgtg ttgctaatga acgtctgatg
    15181 cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg ggccaaagtt acctgaaccg
    15241 gcaacgggga tcgctcagcc gactccgcag caaccgacga caggaaatgc agctactgcg
    15301 cctgctgcgc cgacacagcc tgctgctaac cgctctccac aaagggctac gccgccgcaa
    15361 agtggtgctc aatcgcctgc gcgtgcgccg ggagggcaat agtggcgagc tatcgtagcc
    15421 agggacgttg ggtaatctgg ctctctttcc tcattgcgct gttgctgcaa atcatgccct
    15481 ggccggataa cctgattgtt ttccggccaa actgggtgtt actcatcttg ttgtattgga
    15541 tcctggcctt gcctcatcgc gtaaatgtgg gcacaggttt tgtgatgggt gccatactgg
    15601 atctgatcag cggctcgacg cttggcgtac gcgtattggc gatgagcatc attgcttacc
    15661 tggtggcgct gaaataccag cttttccgca acctcgcatt atggcagcag gcgctggtcg
    15721 tcatgttgct ttcgctggtg gtggatatta ttgttttctg ggcagagttt ttagtgatta
    15781 acgtctcttt cagaccggaa gtgttctgga gtagtgtagt caatggggtg ctctggccgt
    15841 ggattttctt gctgatgcgt aaagtccgtc agcagtttgc agtgcaataa aggtttctat
    15901 gacttctctg tatttagctt ccggttctcc gcgtcgtcag gagttacttg cgcaacttgt
    15961 cgtgaccttt gaacgtattg ttacgggcat tgaggagcag cgtcagccgc aggagagcgc
    16021 gcagcagtat gttgtgcgtc tggcgcgcga gaaagcacag gcgggtgtcg cgcaaacggc
    16081 gcaggatctc ccggtgctgg gtgcggatac tatcgttatc ctgaatggag aagtgctgga
    16141 gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc aaattatcgg gtcagaccca
    16201 tcaggtgatg acggcagtgg cgttggccga cagccagcac attctcgatt gcctggtggc
    16261 caccgatgtg actttcagaa cgttaacaga cgaagacatc gcgggctatg tcgccagcgg
    16321 tgaaccgtta gataaagcag gtgcatacgg tattcagggg ctgggtggct gttttgtcag
    16381 gaagataaat ggcagctatc acgccgtagt cggcttaccg ctggttgaaa cgtatgaatt
    16441 attaagtaat tttaacgcac tgcgtgagaa aagggataaa catgacggct gaattgttag
    16501 taaacgtaac gccttcggaa acgcgagtgg cgtatattga tggcggtatt ctgcaggaaa
    16561 ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa tatctacaag ggtcgtgtaa
    16621 gtcgtgtact tccgggtatg caggcggctt ttgtagatat tgggctggat aaagccgcgt
    16681 ttcttcatgc atccgacatc atgccgcaca ccgaatgtgt ggcgggtgaa gaacaaaagc
    16741 aattcacggt gcgcgacatt tcggaactgg ttcgtcaggg gcaagatctg atggtgcagg
    16801 tggtgaaaga tccgcttggc actaaggggg cgcgcctgac caccgatatc acgctgcctt
    16861 ctcgctatct ggtctttatg ccaggggctt ctcacgttgg tgtttcccaa cgtattgaaa
    16921 gcgaatcaga acgtgaacgc ctgaaaaaag tggtcgcaga gtattgcgac gagcagggcg
    16981 ggtttatcat ccgtaccgca gcggaagggg ttggcgaggc tgaactggcc tccgatgccg
    17041 cttatctgaa acgcgtctgg accaaagtta tggagcgtaa aaaacgcccg cagacccgtt
    17101 atcagctgta cggcgaactg gcgctggcgc agcgtgttct gcgtgatttc gccgacgccg
    17161 aactggaccg cattcgcgtt gactcacgcc tgacttacga agcattgctg gagtttacct
    17221 cggagtacat tcccgagatg accagcaagc tggagcatta caccggacgc cagccgattt
    17281 tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgct ggaacgcaaa gtagaactga
    17341 aatccggtgg ttatctcatt atcgaccaga ccgaagcgat gaccaccgtg gacatcaata
    17401 ccggagcgtt tgtcggtcat cgcaatctgg acgacaccat tttcaatacc aatattgaag
    17461 cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct gggtgggatt atcattattg
    17521 atttcatcga tatgaataat gaagatcacc gccgccgcgt gctgcactcg ctggagcagg
    17581 cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt ttcggcgctg gggctggtgg
    17641 agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt actgtgtaac gaatgcccaa
    17701 cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt atgctatgaa atcatgcgcg
    17761 agattgttcg tgtccaccat gcttacgact ccgaccgttt cctggtctat gcttctccgg
    17821 cagtagctga agccttgaaa ggcgaagagt cacactcgct ggcggaagtg gaaattttcg
    17881 ttggcaaaca ggttaaagta caaattgaac cgctctataa ccaggagcag tttgacgtcg
    17941 taatgatgta aacagatgct gggccgccat ccggcaaagg gtttttgagt cacattttta
    18001 gcagacaagg agtgacgggt gaggcgattg ccggggattt tactgcttac tggagccgcg
    18061 ctcgttgtga tcgctgccct gctggttagc ggcctgcgta ttgctttacc gcatcttgac
    18121 gcctggcgtc cggaaatcct caacaaaata gaatccgcga ctggcatgcc ggtagaagcc
    18181 agtcagctct cagccagctg gcagaatttt ggcccgacgc ttgaagcaca cgacatccgt
    18241 gcagaactaa aagatggcgg cgaattttcg gttaaacgcg ttactctggc gctggatgtc
    18301 tggcagagct tgttacatat gcgctggcag tttcgcgacc tcactttctg gcagctgcgc
    18361 tttcgcacca acactcctat caccagcggt ggtggtaatg atagactgga agccagtcac
    18421 atcagcgatc tgtttcttcg tcaatttgac catttcgatc tccgcgacag tgaagtcagt
    18481 tttctgacgc catccggtca gcgcgccgag ctggcgatcc cacaactcac ctggctgaac
    18541 gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct ctagccttac cggactgcac
    18601 ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg ggttgttaag caatggtcgc
    18661 gtctggctac aggcggatga catcgacctg aagccgtggc tcggtaaatg gatgcaggac
    18721 aatattgctc tggaaacggc acagttctcc cttgaaggct ggatgacgat cgacaaaggc
    18781 gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg ccagctggtt gggcgagaag
    18841 caaacgcata cgctgtcggt ggataatctg accgcgcata ttacgcgtga aaatccgggc
    18901 tggcagttct ctattcccga tacacggatc acgatggacg gcaaaccctg gccgagcgga
    18961 gcattgacgc tggcctggat accggaacag gacgttggcg gcaaagacaa taaacgcagt
    19021 gacgaactcc ggattcgcgc cagtaatctg gagctggcag gcctggaggg catacgcccg
    19081 ctggccgcga aactttcacc tgcactgggt gatgtttggc gctccacaca accgagcggc
    19141 aagattaaca ctctggcgct ggatatcccg cttcaggcgg cagacaagac ccgttttcag
    19201 gcatcgtgga gcgatctggc ctggaagcaa tggaaattat taccgggtgc ggaacacttc
    19261 tccgggacgc tttccggcag cgttgaaaat ggtttgctta ccgcgtcgat gaagcaggca
    19321 aagatgcctt acgaaacggt attccgtgcg ccactggaaa tcgccgacgg ccaggcaact
    19381 ataagctggc tgaacaatga caaaggtttc cagctggatg ggcgtaatat tgacgttaaa
    19441 gccaaagccg tccatgcgcg cggcggtttt cgttacctgc aacctgctaa cgatgaaccc
    19501 tggctgggta ttctggctgg catcagtacc gatgatggtt cacaagcctg gcgctatttc
    19561 ccggaaaact tgatgggtaa agacctggtt gattacttaa gtggcgcgat tcagggcggt
    19621 gaagcggata acgcgacgct ggtttatggt ggcaatccgc aactcttccc ctataaacac
    19681 aacgaaggtc agtttgaagt gctggtgccg ctgcgtaacg cgaagtttgc cttccagccg
    19741 gactggcctg cattaactaa ccttgatatt gaactggact ttattaacga cggtttatgg
    19801 atgaaaaccg atgacgttaa tctgggcggc gtgcgcgcga gtaatctcac cgcagtgatc
    19861 cctgactact cgaaagaaaa actgctgatt gacgctgaca ttaaaggtcc gggtaaagcc
    19921 gttggccctt actttgatga gacaccgctg aaagattctc tgggtgcgac cctgcaagaa
    19981 ctccagctcg acggcgatgt gaatgctcgc ttacatcttg atatcccgct gaacggcgaa
    20041 ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca gtctgtttat caaaccactc
    20101 gacagcaccc tgaaaaattt gagcggtaaa ttcagcttta tcaatggcga tctgcaaagt
    20161 gaaccactga cagcaagctg gtttaatcag ccgttgaacg tggatttttc caccaaagaa
    20221 ggggcaaaag cctaccaggt tgcggtaaac ctcaacggta actggcaacc ggcgaaaacc
    20281 ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca gcgtggcgtg ggatggtaaa
    20341 gtgggcattg agctgcctta tcatgctggc gcgacctata acgtagagct gaacggcgat
    20401 ctgaagaatg tgagcagtca cttaccttca ccgttagcca aacctgcggg tgaaccacta
    20461 gcggtaaacg ttaaggttga tggcaatctc aacagctttg aattaaccgg acaggctggt
    20521 gcggataacc atttcaatag ccgctggttg cttggtcaaa agctgacgct cgatcgtgct
    20581 atttgggcgg cagacagtaa aacgctcccg ccgttgccgg aacaaagtgg cgttgaactc
    20641 aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt tccagaaagg cgctgcggag
    20701 agtgtcggtg gtgcagcgag tttcccacaa cacataacgt tacgtacgcc tatgttgtca
    20761 ctgggaaatc agcaatggaa taacctgagt attgtttcgc aaccgacggc aaatggcacc
    20821 caggttgagg cgcaagggcg tgaaatcaac gccacgctag cgatgcgtaa taacgcgccg
    20881 tggctggcga atatcaaata tctttattac aacccgagcg tggcgaaaac tcgtggtgat
    20941 tcaacgccgt catcaccttt cccgacaacg gagcgcatta acttccgtgg ctggccggac
    21001 gcacaaatac gatgcacaga gtgctggttc tgggggcaaa aattcggtcg cattgacagt
    21061 gatctcacca tttctggcga tacgttaacg ctgaccaatg gactgattga tactggtttc
    21121 tcgcggctta ctgccgatgg tgaatgggtt aataatccgg ggaatgaacg tacctcgctg
    21181 aaaggaaaac tgcgcgggca gaaaattgat gccgccgcag aattttttgg tgtcacgacg
    21241 cccatacgcc agtcgtcatt taatgtggat tacgatttac actggcgcaa agcaccctgg
    21301 cagccagatg aagcgacgtt gaatggcatc attcatactc aactgggtaa aggcgaaatt
    21361 accgaaatca ataccggaca tgccgggcaa ttgctgcgct tattgagcgt agatgccctg
    21421 atgcgtaagc tgcgttttga tttcagagac acttttggcg aagggttcta ttttgactcc
    21481 attcgcagca ccgcgtggat taaagacggc gttatgcaca ccgacgacac gctggtggat
    21541 ggcctggagg cggatatcgc catgaaaggg tcggtaaatc tggtacgtcg cgacctgaat
    21601 atggaagcgg ttgtcgcacc agagatttct gcgacggtgg gcgtggctgc ggcttttgcg
    21661 gttaacccca ttgttggcgc ggcagtgttt gccgccagta aagtgctggg gccgctgtgg
    21721 agcaaagtct ccattttgcg ctatcacatt tcgggtccgc tggacgatcc gcaaatcaac
    21781 gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat ttgacgaggg cgcgtaattg
    21841 ccccaatctc atatgataat cgttgccaaa ggccaacgag ccggaacata actgtaggcc
    21901 ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat cctctactgc agtaactaac
    21961 gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca attgctagcg gcgaacggcc
    22021 tgaaacatca ggacttgttc gcgatcctcg gtcaactggc cgaacgtcgc cttgattatg
    22081 gcgatctcta ttttcagtcg agctatcacg aatcctgggt tttagaagac cgcattatta
    22141 aagatggttc ttacaacatc gatcagggcg ttggtgtgcg tgcaatcagt ggtgaaaaaa
    22201 ccggatttgc ttacgctgac caaatcagcc tgctggcgct ggaacagagt gcgcaagcgg
    22261 cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca gacgctgggc gcggtagagc
    22321 atagcccgtt gtatacctcg gtagatccgc tgcaaagcat gagccgtgaa gagaagctgg
    22381 atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga caagcgcgta caggaagtga
    22441 ctgccagcct cagtggtgtc tatgaattaa ttctggttgc ggccaccgac ggcacgctag
    22501 cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt tctggtcgaa gaagatggca
    22561 aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg ttatgagttc ttccttgccg
    22621 atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga agcagtgcgt atggcgctgg
    22681 tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc ggtagtactt ggcgcaggtt
    22741 ggccgggcgt gctgttgcat gaagcggtag gtcacggtct ggaaggcgac ttcaaccgcc
    22801 gcggcacttc agtatttagt gggcaggtcg gggagctggt ggcttcagaa ctgtgtaccg
    22861 tggttgatga cggcacgatg gtcgatcgcc gtggttcggt ggcgattgat gacgaaggta
    22921 cgccaggcca gtacaacgtg ctgattgaga acggcattct gaaaggctac atgcaggata
    22981 aactcaacgc gcgtttgatg gggatgacgc cgactggcaa cggtcgccgt gaatcctacg
    23041 cccatctgcc catgccgcgt atgaccaaca cctatatgct gccgggtaaa tcgaccccgc
    23101 aggaaattat tgaatccgtt gagtacggta tctatgcacc gaactttggt ggcggtcagg
    23161 tggatatcac ctccggcaaa ttcgttttct ccacttcaga agcatatctg attgaaaacg
    23221 gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg ttccggtatc gaaaccatgc
    23281 agcagatttc gatggttggc aacgacctga aactggataa cggcgtgggt gtctgcggta
    23341 aagaagggca aagtttgccg gttggcgtgg gccagccaac gctgaaagtt gataacctga
    23401 ctgttggcgg tactgcgtaa taatttcatt attttcagaa ggataattaa tctttctaca
    23461 tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag gggaacccgt tggcacaggt
    23521 ttgtagaacg taacagtaca atatgaatta cttctctttc ccgcgcccgt gcatctcctg
    23581 aaacaattta ccgacctcaa caaaataatc cgtcagcgag ttgatcacga cctgtacctt
    23641 cagcggcagc ttatcttttt cggtatataa cgcataaacc gggcgtggat ctgactgata
    23701 acgcggcagc aggatctcca gctcaccgcg attgatctcg ttgatcaccc acatcagcgg
    23761 cacgtaggcg atcccggcac ccgccgttag ccagcgcacc agcgtcatcg gatcattagt
    23821 cacaaatctc ccttgcggga tcaggcgagt cgagatccct tccggtgcaa tcagttcaaa
    23881 ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg ctacttaaat cggcgggttt
    23941 ttccggtatg ccgtattgtg tgagatagct tttcgcggcg cacaccacca ttggcatcgc
    24001 gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg ccgacgcgga tcaccacatc
    24061 cagaccgtcg gcaatcaggt cgggggctgg aattccggta accagattga cgctcaaacc
    24121 tgggtattct ttcagcattt ttgctgtcag cccggcgaga acattttgtg ccatagttga
    24181 agaacagcca atgcgcagcg tcccgatggg ggtgttattg aaggcataca gttgctcatg
    24241 aacatcctgc acttcatgaa gcatccgacg gcagccctgg tagtaaattc tgccggcttc
    24301 ggtcaggcca atgctgcgtg tgctacggtt taacagcttt acctgcaact catcttccag
    24361 ttttgacacc gtctgactga tggacgaaac gctcatctgt agctgtctgg cggcggcggt
    24421 aaaagagcca aattcaacta ctttggcaaa caccgacatg cgttttagtc gttccattat
    24481 tcactctgac ttaaaagtga tttagatcac ataatataga taacagcata acagttacgc
    24541 taatatatta aatatcaatc tacagcaatg ttgctctcgc ccggctttcc atgcccttct
    24601 ctgcggcgac agatgctgaa aataataacg cctgctctct ctttacaacc aaggtcaaca
    24661 tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt cccaccgata ttttttgaat
    24721 tgcttttatc actggcgatt ttctggctgg tgcgccgggt acttgtgcca acaggtatct
    24781 acgactttgt ctggcatccg gcgttgttca acaccgcgct ctattgctgc ttgttttatt
    24841 tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac taataagaaa attctcccgt
    24901 acggccatca cggtcgtatt agtcattctg gccttcatcg caatttttaa tgcctgggtc
    24961 tattacaccg aatccccctg gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc
    25021 gcgccggacg tttctggact cattacccag gtgaatgttc atgataacca gctggtgaaa
    25081 aaaggacaga tactgttcac catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg
    25141 caagccgatg ttgcttatta tcaggtactg gcacaggaga aacgccagga ggccggacgt
    25201 cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga tcgaccaggc taacaacgta
    25261 ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg
    25321 gatcttgaac gcacggtgat ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc
    25381 tataccggtg agtttattac tcgaggatca acggcggttg cgctggtgaa acagaactcc
    25441 ttctatgtac tggcctatat ggaagaaact aagctggaag gggtgcgtcc ggggtatcgt
    25501 gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc
    25561 gcaggggtca ccaacgccag cagcacgcgt gacgacaaag ggatggcgac tatagactct
    25621 aaccttgaat gggtgcgtct tgcgcaacgt gttccggttc gtattcgtct cgacaaccag
    25681 caagagaaca tctggcctgc gggcaccact gctacagtgg tggtcactgg caaacaagat
    25741 cgcgacgaaa gccaggattc gttcttccgt aaaatggccc atcgcctgcg tgagtttggt
    25801 taatcacgat gggtattttc tccattgcta accaacatat tcgctttgcg gtaaaactgg
    25861 cgaccgccat tgtactggcg ctgtttgttg gctttcactt ccagctggaa acgccacgct
    25921 gggcggtact gacagcggcg attgttgccg ccggtccggc ctttgctgcg ggaggtgaac
    25981 cgtattctgg tgctattcgc tatcgtggct ttttgcgcat catcggcaca tttattggct
    26041 gtattgccgg actggtgatc atcattgcga tgatccgcgc accattattg atgattctgg
    26101 tgtgctgtat ctgggctggt ttttgtacct ggatatcctc gctggtacga atagaaaact
    26161 cgtacgcgtg ggggctggcc ggttataccg cgctgatcat tgtgatcacc attcagccgg
    26221 aaccattgct tacgccgcag tttgccgtcg aacgttgtag cgagatcgtt atcggtattg
    26281 tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc gatcaaacaa gaagtggatc
    26341 gagagctgga aagtttgctg gtcgcgcaat atcaattaat gcaactctgt atcaagcatg
    26401 gcgatggtga agttgtcgat aaagcctggg gcgacctggt gcgacgcacc acggcgctac
    26461 aaggcatgcg cagcaacctg aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt
    26521 taaaagcgat caatacgcta tcgctgacgc tgattaccca atcctgcgaa acttatctta
    26581 ttcagaatac gcgcccggaa ttgatcactg atactttccg cgaatttttt aacacgccgg
    26641 tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct gcggagagtt atcgcctgga
    26701 ccggggaacg ggaaacgcct gtcaccattt atagctgggt cgcggcggca acgcgttatc
    26761 agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc
    26821 tgcaaggcga accggaagtc aaagtagagt cagccgaacg tcatcatgcg atggttaact
    26881 tctggcgaac cacactttcc tgcattctgg gcacgctttt ctggctgtgg acgggctgga
    26941 cttccggcag tggtgcaatg gtgatgattg cggtagtgac gtcactggca atgcgtttgc
    27001 cgaatccacg catggtggcg atcgacttta tctacgggac gctggccgcg ctgccgttag
    27061 ggctgctcta ctttttggtg attatcccta atacccaaca gagcatgttg ctgctgtgta
    27121 ttagcctggc agtgctggga tttttcctcg gtatagaagt acagaaacgg cgactgggct
    27181 cgatgggggc gctggccagc accataaata ttatcgtgct ggataacccg atgactttcc
    27241 atttcagtca gtttctcgac agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca
    27301 ccgttatttt gcttgtgcgg gataaatcgc gcgacaggac cggacgtgta ctgcttaatc
    27361 agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc
    27421 acctcccggc actttatcag cagctgtttt tgctgatgaa taagttccca ggggatttgc
    27481 cgaaatttcg cctggcgctg acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga
    27541 tcccggttaa cgaggattta tcggcgtttc accgacaaat gcgccgcaca gcagaccatg
    27601 tgatatctgc ccgtagcgat gataaacgtc gtcggtactt tggccagttg ctggaagaac
    27661 tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg
    27721 ttcatcggct ggcggggatg ctccataagt atcaacatgc gttgaccgat agttaagtca
    27781 aaaccgacgc caaaagcgtc ggttttttca tggctatact tagcgatgca cggcagaact
    27841 cgccgcgaaa cgtgacggtg gcaacagatg aatatttata cctttgattt tgatgagatt
    27901 gagagtcagg aggattttta tcgtgacttt agccaaacct ttggtctggc taaagataag
    27961 gtacgcgatc tcgactcact atgggatgtg ttaatgaacg atgtcctgcc gctaccactt
    28021 gagattgaat ttgttcatct gggagagaaa acgcgtcgcc gttttggcgc gttaatattg
    28081 ctgtttgatg aggcagagga agagctggaa gggcatttgc gttttaatgt tcgtcattag
    28141 cgcaaaaaaa agcccccgaa ccgggggcaa tatcgtcgga caagacgatg agggtttatt
    28201 tgtacagttc agccgtagcg tgccaggtgt caccgctacg agcttcagta atctggtagg
    28261 ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc acgcatatcc attggcgaag
    28321 acgccacacc acttacggat acggtcccga ttgcttcacg attttgtgct tgtgcagcat
    28381 caatggagtc ggcagcgaat gcaccgaaag agagaacaga aagtacgctt aatgcagcta
    28441 cagtggtttt gattttcatg atttttacct cgacataatc ttttagctgg gcctttgttt
    28501 cgtgacccat atcacaaaat caagtataca ctaattattg aactaattaa t
//
LOCUS       DAZKIV010000046        28236 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZKIV010000046 DAZKIV010000000
VERSION     DAZKIV010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.046"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..1140)
                     /gene="gldA"
                     /locus_tag="ACHOT6_001992"
     CDS             complement(37..1140)
                     /gene="gldA"
                     /locus_tag="ACHOT6_001992"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFP9947215.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(1151..1813)
                     /gene="fsa"
                     /locus_tag="ACHOT6_001993"
     CDS             complement(1151..1813)
                     /gene="fsa"
                     /locus_tag="ACHOT6_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9947216.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(1825..4326)
                     /gene="ptsP"
                     /locus_tag="ACHOT6_001994"
     CDS             complement(1825..4326)
                     /gene="ptsP"
                     /locus_tag="ACHOT6_001994"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9947217.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSGSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            4635..5714
                     /gene="frwC"
                     /locus_tag="ACHOT6_001995"
     CDS             4635..5714
                     /gene="frwC"
                     /locus_tag="ACHOT6_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFP9947218.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            5729..6049
                     /gene="frwB"
                     /locus_tag="ACHOT6_001996"
     CDS             5729..6049
                     /gene="frwB"
                     /locus_tag="ACHOT6_001996"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9947219.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            6100..8397
                     /gene="pflD"
                     /locus_tag="ACHOT6_001997"
     CDS             6100..8397
                     /gene="pflD"
                     /locus_tag="ACHOT6_001997"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFP9947220.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            8363..9241
                     /gene="pflC"
                     /locus_tag="ACHOT6_001998"
     CDS             8363..9241
                     /gene="pflC"
                     /locus_tag="ACHOT6_001998"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFP9947221.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            9243..9584
                     /gene="frwD"
                     /locus_tag="ACHOT6_001999"
     CDS             9243..9584
                     /gene="frwD"
                     /locus_tag="ACHOT6_001999"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFP9947222.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(9571..10422)
                     /gene="yijO"
                     /locus_tag="ACHOT6_002000"
     CDS             complement(9571..10422)
                     /gene="yijO"
                     /locus_tag="ACHOT6_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9947223.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLISHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10571..12304)
                     /gene="eptC"
                     /locus_tag="ACHOT6_002001"
     CDS             complement(10571..12304)
                     /gene="eptC"
                     /locus_tag="ACHOT6_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9947224.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(12487..15138)
                     /gene="ppc"
                     /locus_tag="ACHOT6_002002"
     CDS             complement(12487..15138)
                     /gene="ppc"
                     /locus_tag="ACHOT6_002002"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFP9947225.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(15490..16641)
                     /gene="argE"
                     /locus_tag="ACHOT6_002003"
     CDS             complement(15490..16641)
                     /gene="argE"
                     /locus_tag="ACHOT6_002003"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFP9947226.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            16795..17799
                     /gene="argC"
                     /locus_tag="ACHOT6_002004"
     CDS             16795..17799
                     /gene="argC"
                     /locus_tag="ACHOT6_002004"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFP9947227.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            17807..18583
                     /gene="argB"
                     /locus_tag="ACHOT6_002005"
     CDS             17807..18583
                     /gene="argB"
                     /locus_tag="ACHOT6_002005"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFP9947228.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            18644..20017
                     /gene="argH"
                     /locus_tag="ACHOT6_002006"
     CDS             18644..20017
                     /gene="argH"
                     /locus_tag="ACHOT6_002006"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFP9947229.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            20284..21201
                     /gene="oxyR"
                     /locus_tag="ACHOT6_002007"
     CDS             20284..21201
                     /gene="oxyR"
                     /locus_tag="ACHOT6_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFP9947230.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(21184..22584)
                     /gene="sthA"
                     /locus_tag="ACHOT6_002008"
     CDS             complement(21184..22584)
                     /gene="sthA"
                     /locus_tag="ACHOT6_002008"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFP9947231.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALNCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            22915..23565
                     /gene="fabR"
                     /locus_tag="ACHOT6_002009"
     CDS             22915..23565
                     /gene="fabR"
                     /locus_tag="ACHOT6_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFP9947232.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            23565..23924
                     /gene="yijD"
                     /locus_tag="ACHOT6_002010"
     CDS             23565..23924
                     /gene="yijD"
                     /locus_tag="ACHOT6_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFP9947233.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(23964..25064)
                     /gene="trmA"
                     /locus_tag="ACHOT6_002011"
     CDS             complement(23964..25064)
                     /gene="trmA"
                     /locus_tag="ACHOT6_002011"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFP9947234.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      25178..25368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            25433..27277
                     /gene="btuB"
                     /locus_tag="ACHOT6_002012"
     CDS             25433..27277
                     /gene="btuB"
                     /locus_tag="ACHOT6_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFP9947235.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLVDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            27222..28079
                     /gene="murI"
                     /locus_tag="ACHOT6_002013"
     CDS             27222..28079
                     /gene="murI"
                     /locus_tag="ACHOT6_002013"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFP9947236.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         6466 a         7106 c         7772 g         6892 t
ORIGIN      
        1 ttcaaactcc cggacaagcc gggagtttgg agtaggttat tcccactctt gcaggaaacg
       61 ctgaccgtac tggtcggcta ccagcagagc ggcgtaaacc tgatctggcg tcgcgccgcc
      121 aggcatgttg tggatggttt caccttctgc acatgccgct tctgccacaa ttcgcatttt
      181 cgccgggaca tcttctttaa tatccagttg agcgagagtt attggcaaac ctaccgcatg
      241 gctaagtgca gctacggttt cgatttcctc aaccggcgcg ttttccagta ccagctgcgt
      301 cagcgtaccg aatgccactt tttcaccgtg ataatagtga tgcgcgtccg ggatagcggt
      361 caggccgtta tgcaccgcat gagccgccgc cagtccgccg ctttcaaaac caacgccgct
      421 taaataggtg ttcgcttcaa tcacgcgttc cagcgcaggg gttactacat gctgttcggc
      481 ggcaagcatc gctttttcgc cttcttccag cagagtgttg tagcacagtt ctgccagtgc
      541 cagcgccgcc tgggtgcact tgccgcccgc catagtggta gcgccgctac gggaacaggc
      601 gcgtgcttca aaccaggttg ccagcgcatc gccgataccc gccgctaaca gacgtgcagg
      661 agcgcccgcg acgattttgg tgtcgacaat gaccatattc gggttatttg gcaacagtag
      721 atagcggtca aactcaccct catcggtgta gataacagac aatgcgctgc acggtgcatc
      781 ggtagaggcg atagtcggtg cgatcgctac cggaacaccc atgaaatgtg ccagtgcttt
      841 ggcagtatcg agggtttttc cgccaccgat accgagaatt gcgccacact gcgcagtctc
      901 cgcgatgcca cgcagacgat cgatctcatt ttgcgaacat tcaccgccaa acggcgcaat
      961 ttctactacc agtccagcat ctttaaagct tttctcgaca gtggattgag caaaacctaa
     1021 aacaaatttg tcacccacca ctaaccagcg ttctgccagc ggcttcaggt attcgcccag
     1081 acgattaatc acatcagcgc cctggatgta tttacccggt gattgaataa tgcggtccat
     1141 aattgctcct ttagagatga gtagtgccaa atgcggcatt ccagtcgtgt tcgaacttct
     1201 ctatagctga ctctaccgca ggggtgttga gcatttgttg cgctacatct aagggcaggg
     1261 tgatggattc acatcctgcc agtaaacagt ccagcgcctg acgcggcgtt ttaaagctgg
     1321 ccgccagcac catgctttct ggtgcgtgca tttctaacag cgcttgcagc tcctgaaccg
     1381 tacgaatgcc gtccccgccc tgggcatcta cgcggttaac atacggagca acgtattttg
     1441 cccctgccag cgcggccagt aacccttgtg cagcactgta cactgcggtg ccaagcgtag
     1501 taataccctc ttttttcagc atcttaatgg cggccagacc ttcggaagtc accgggattt
     1561 tcaccacaat gcccggaatg gcgtcgcgca ggcgcttcgc ttcttccacc ataccctgcg
     1621 cgtcacgact catagtctga gcaaacagaa tgccctcatc accaatcgct ttttgcagac
     1681 gcggcagaac ttcccatatg gactccttgc tggcagcgat aatgctcggg ttagttgtca
     1741 ccccggcaat ggggaatatg cgtgccagac gttcgacttc tgcgacgtta gcggtgtcca
     1801 gatacagttc catgatgttt ccttttacag ttccagttca tgttgcagca ggctggcgat
     1861 agcgtcggca gaggcggcgt taaccagcgc gttacggaat tcttcgtgca tgatgcggcg
     1921 agcgaggcgc gagaaaatgc gcatgtgctg atcgcccgca gcgtgtttgt tcagggttaa
     1981 cataatgatg aattgcgctt catcatcgcc ccagcgcacc ggcgcttgca gacgcgccac
     2041 gctgatggtg gattgctcaa tgtgttctga tttgctgtgt ggaatggcaa aactaaagcc
     2101 cagaccggta gagaaaacgg cctcgcgcgc ccataagtcg gcttccagtt tacgcggata
     2161 gcggcagcgg cccgccagca gcaggttatc ggtcatgcct ttgagcactt cttctttgct
     2221 gcgccagtca ctttccagcg tgatgcactc ggcggtgacc agcggtgcgt cctgttgggt
     2281 catgcggaat tgcgccagca ggtgttccac ttccagcgaa gtacggcagg ccattgcctg
     2341 gttgagcaac ttgcggcact cacggctatc aagttgcgcc atccgcgctt tcgccgccgg
     2401 aattgatggt gcgctcatgc tgagttcatc cagccctaag ccgaccagca acggcagcac
     2461 ggaacctttc gctcccagct caccgcacag accaatccat ttgccctggc gatgcaccgc
     2521 ctgcacggcg taatcgagcg cccgcaagaa tgccggattc aggctgttgt agtgacgagt
     2581 aaccttagcg ttatcgcgat ccactgccag cagatattgc gtcaggtcat tactaccaat
     2641 actaaagaaa tcaatctctt cgcagcattg atcgatgatg aacatcaccg acggcacttc
     2701 cagcataatg ccgagctgga tcttctcatc aaacggaatg tgttcgttac gtagttgctg
     2761 tttggcttct gccagttttt ctttcaccca taagatctct tccattgagg agatcatcgg
     2821 gatcatgatt ttcaggctgc cgtgagcgga ggcgcgcagg atcgagcgta gttgtgtggt
     2881 aaataacgac gcgtattctt cataaatacg cacggcgcga taaccgagga acgggtttgc
     2941 ctctgcggga atgtttagat aatcaacggg tttgtcgccg ccaatgtcca tagtgcgcac
     3001 aataatgctg cgtccgttgg cagactccag cgcctgacaa aaaatgttgt acagctcgct
     3061 ttcgcccggt gcactggtgc gatccatata gagcatttca gtgcggaaca aaccaacgcc
     3121 ttccgcacca ttgccgaatg cggcctgcgc ttccacggag tgagcgatat tagcggcaat
     3181 ttcaatgcgg ataccgtcag cggtacgggc ttgttgggtc agccagacac gctgttgctc
     3241 acgcagggcg tcctgtacgc gggcttcttg ctgataataa cgagctacgg cttctcctgg
     3301 ctcaaccaca atcgccccag cgttgccgtc gatataaatc gtttgatgct gccacggagt
     3361 aagggcatca atatctacac caaccagtgt cggaatgttg aacgaacggg caagaattac
     3421 ggtatgtgag gtggtgccgc cacttttcag taacagacct ttgaggtgat ttttatccag
     3481 ttcgaggaac tggctgggag tcagttcatc agccatacaa atggcgggct gcgtcagttt
     3541 gcccggtgcc gggaagcgtt gctcaccgta gatttgctgg agtaactgga agcagacgtc
     3601 gcgtacgtcc agggcacgtt cttgcaggta gctgctgccg gaacgggcga actcttcgca
     3661 aaagtgattc gcactggtga caatcgcttc ggcgcagctt aatccggcgc tgacgcctgc
     3721 cagtaaatgt tcgcgcaggg aagtatcgcc agccagggat cggtgcgctt ccagaatcgc
     3781 gctggtggca ccgtcgctat ccagcagacg aaactcaatg tttttcagta ccagcgtcag
     3841 gccgttttcc agtgcggatt gctcggcgtc aacacctttg gctgcgggaa gattacccag
     3901 cgcattgaga tctaaagaag agatcggcgt cagaatgccg cctgcactac cgctgcacac
     3961 ggtgcgggcg cggataattt gcggattcag attggtcagt gaaaccggca gtggttccag
     4021 ttcgtcagat ttaacttccg ccagcggcgc atcgcagtgg gggaattcat cgcgcagcca
     4081 ttggcttaaa cgctggtgcg cttcctgttc gtcggccccg gaaatcagta actggcagtt
     4141 atcgcccgcc agcgtatcgg tgccaatcag cgcaagggcg cttttggcgt tgcccttgcg
     4201 gtcagtgcgc aggttatgcc actcaatttg tgatgaaaaa gtattacaca gcgtttcaac
     4261 gtggcttgcc ggacgcgcat gtacgccgtt aggtagctca caaataaatt ccacaatcag
     4321 ggccattgcc tctctcccgg tacggttttc tgtttacagg ataagagcgc acggcaacgg
     4381 cctgccatgt gacaaatctg ccaaaagctg gacaaatgta atgtaaccgt caatttgcga
     4441 cgcgtctcac aagacgccgt tttgcggcat gcttccggat tagcgcaagt tatgaggcgg
     4501 atcgcatttt tgtactgata ttacaaaagt ccagtaaatg gcctttttat ccactgtttg
     4561 ctccgcctgc gattgcctat tgtttcctcc atcaaaaata tggacatggg cttttgcaag
     4621 cccgggagca gcttatgaat gagttggtgc agatcctgaa aaatacccgt cagcatttaa
     4681 tgacgggcgt ttcacacatg attcccttcg tggtatcggg cggtattttg ctggcggttt
     4741 ccgtcatgtt gtatggcaaa ggcgcagtgc cggatgccgt agccgatccg aatttgaaaa
     4801 aactgtttga tatcggcgtt gcaggtttga cgctgatggt gcctttcctc gccgcttaca
     4861 ttggttactc cattgcagag cgttctgcgc tggctccgtg cgctatcggt gcctgggttg
     4921 gtaacagctt tggtgcgggc ttctttggtg cgctgatcgc cgggattatc ggcggcatcg
     4981 tggtgcatta cctgaagaaa attccggtgc ataaagttct gcgctcggtg atgcccatct
     5041 tcattattcc tatcatcggc acactgatta ccgcaggcat catgatgtgg ggcttgggcg
     5101 agcctgtagg ggcgttgacc aacagcctga ctcagtggct tcaggggatg cagcagggca
     5161 gcattgttat gctggcggtg atcatgggtc tgatgctggc gttcgatatg ggcggtccgg
     5221 ttaacaaagt ggcctatgcc ttcatgctga tttgcgttgc tcagggtgtt tataccgtgg
     5281 tggccatcgc tgccgtgggt atttgtatcc cgccgctggg gatggggctg gcgacgctga
     5341 ttggtcgtaa aaatttctcc gcagaagagc gcgaaaccgg caaagcggca ctggtgatgg
     5401 gctgtgttgg cgttactgaa ggggcgattc ctttcgccgc tgccgatccg ctgcgtgtca
     5461 ttccttccat catggtcggt tcagtttgtg gtgcagtaac tgcggcgctg gtcggtgcgc
     5521 agtgctatgc aggctggggt ggtctgattg tgctgccggt ggttgaaggc aagctgggtt
     5581 atatcgcagc agtggctgtc ggagcagtgg tgacggctgt ttgtgtgaac gtgctgaaaa
     5641 gtctggcgcg taaaaatggg tcttcgactg atgaaaaaga agacgacctg gatttggatt
     5701 ttgaaattaa ttaattgagg aacgagccat gacgaaaatt attgcagtaa ccgcatgtcc
     5761 ttcaggtgtt gcccatacct atatggcagc agaggcgctg gaaagtgccg cgaaagcgaa
     5821 aggctgggaa gtgaaagtag aaacccaggg gtcaattggt ctggaaaatg aactgactgc
     5881 ggaagatgtg gcgagcgctg atatggtgat tctgaccaaa gatatcggca tcaagtttga
     5941 agagcgtttt gctggcaaga ccatcgtgcg cgtcaacatc agcgatgcag taaaacgtgc
     6001 tgacgccatt atgagcaaaa ttgaagctca cctggcgcaa accgcttaag ttcttctccc
     6061 gctcgcaagg gcgggttcgc tttcccacag gagttcctca tgacgaatcg tatctctcgc
     6121 ctcaaaactg cactgtttgc caatacccgt gaaatctcgc tggagcgggc gttgctttat
     6181 accgccagcc atcggcaaac cgaaggcgaa ccggtgatat tgcgccgggc gaaagcaaca
     6241 gcgtatatcc ttgaacatgt tgaaatttcg attcgtgatg aagaactgat tgccggtaac
     6301 cgcaccgtaa aaccgcgcgc tgggattatg tcgccggaaa tggaccctta ctggctgctg
     6361 aaagagctgg atcaattccc gacgcgtccg caggaccgct ttgctatcag tgaagaagat
     6421 aaacgtatct accgtgaaga gttgttcccg tactgggaaa aacgttcgat gaaagatttc
     6481 atcaacgggc agatgacaga tgaagtaaaa gccgcgacca gcacgcagat tttcagcatc
     6541 aaccagacag ataaaggcca ggggcacatt attattgatt acccacgcct gctgaatcac
     6601 gggctggggg agctggtggc gcagatgcag caacattgtc agcaacagcc ggagaatcac
     6661 ttttatcagg ccgcgctgtt actgctggaa gcctcgcaga aacacattct gcgttacgct
     6721 gaactggcgg aaacaatggc ggcaaactgc acagatgccc agcgtcgcga agaactgctg
     6781 actattgcgg agatctcccg ccataacgcc gaacataagc cgcagacgtt ctggcaggcg
     6841 tgccagttat tctggtacat gaacatcatt ctgcaatacg aatccaacgc cagttcgcta
     6901 tcgttggggc gcttcgacca gtatatgttg ccgttctatc aggcatcttt aacccagggc
     6961 gaagatccgg cgttcctgaa agaactactc gaatctttat gggtgaaatg caacgacatc
     7021 gtgctgttgc gctccaccag cagcgcgcgt tatttcgcag gtttcccgac cggctatacc
     7081 gcactgctcg gcgggttaac cgagaacgga cgtagcgcgg tgaacgtgct ttcgttcctt
     7141 tgccttgacg cttatcaaag cgtgcaatta ccgcaaccga acctcggcgt gcgcactaac
     7201 gccttgatcg acacgccgtt cctgatgaaa accgccgaaa ccattcgcct cggcaccggt
     7261 attccgcaaa tctttaacga tgaagtggtg gtaccagcgt tcctcaaccg tggcgtttcg
     7321 ctggaagatg cgcgcgacta ttccgtagtg ggctgtgtgg aattatctat tcccggcaga
     7381 acctacggct tgcatgacat cgcgatgttt aatctgctga aagtgatgga aatctgcctg
     7441 catgaaaatg aaggcaatgc tgcgctgact tatgaaggtt tactggagca gatccgcgcc
     7501 aagatcagcc actacatcac cctgatggtt gagggcagca atatttgtga tatcggccat
     7561 cgcgactggg cacctgtacc gctgctctcg tcttttatca gcgattgtct ggaaaaaggt
     7621 cgcgatatta ccgatggcgg cgcgcgttat aacttctccg gcgtacaggg gatcggtatc
     7681 gccaacctga gcgattctct ccatgcgttg aaagggatgg tttttgagca acagcgttta
     7741 agttttgacg aattgctgtc ggtattaaaa gccaactttg caacgccaga aggcgaaaaa
     7801 gtccgtgctc gcttaattaa ccgcttcgag aaatacggta acgatatcga cgaggtggat
     7861 aacatcagcg ccgaactgtt acgccactac tgcaaagaag tggaaaaata ccagaacccg
     7921 cgcggcggtt acttcacgcc gggatcgtat accgtttctg ctcacgtccc gttgggatcg
     7981 gtggttggcg cgacgccaga cggtcgtttt gccggggaac aactggcaga cggcggcttg
     8041 tcacctatgc tgggccagga cgcacaaggg ccaacagcgg tactgaagtc agtcagtaag
     8101 ctcgataata cgctgctgtc taacggtacg ttgctgaacg tgaaattcac tccggcgacc
     8161 ctggaaggtg aagcgggatt acgcaaactg gccgacttct tacgggcgtt tacccagctt
     8221 aagttgcagc atattcagtt taacgtggtg aacgccgaca cgttgcggga agcgcaacag
     8281 cgcccacaag attatgccgg gctggtggtg cgcgttgccg gatacagcgc cttctttgtc
     8341 gaactgtcga aggagatcca ggatgacatc atccgccgga cagcgcatca gctgtaacgt
     8401 tgtggaaacg cgccgcgatg atgtggcgcg cattttcaac attcagcgtt attcactgaa
     8461 tgacggcgag ggcattcgta cggtggtctt ttttaaaggc tgtccgcatc tttgcccgtg
     8521 gtgtgctaat ccggagtcga tctccggcaa aatccagacg gtacgcagag aggcgaaatg
     8581 tctgcactgt gcgaaatgtt tgcgtgatgc ggatgaatgc ccctccgggg cgtttgaacg
     8641 gattggccgc gatatcagcc ttgacgcgct ggaacgggaa gtgatgaaag atgacatttt
     8701 ctttcgcacg tccggcggcg gcgtcacgct ttctggcggc gaagtgttaa tgcaggcgga
     8761 gtttgctacc cgttttttac agcggctgcg gttgtggggc gtgtcatgcg ccattgaaac
     8821 tgccggagac gcgccagcca gcaagctatt accactggcg aaattgtgcg atgaagtgct
     8881 gttcgattta aaaattatgg acgcgactca ggcgcgggat gtggtgaaga tgaacctgcc
     8941 acgcgtgctg gagaatctgc gtttgctggt gagtgagggt gtcaacgtga tcccgcgttt
     9001 accgttgatc cctggtttca cgctcagccg ggagaatatg cagcaggcgc tggatgtact
     9061 gatcccgctg aaaatcaggc agatccatct gttaccgttt catcagtacg gcgaaccgaa
     9121 ataccgcctg ctggggaaaa catggtcgat gaaagaggtg cctgcgccgt cgtcagccga
     9181 tgtgacaacg atgcgcgaaa tggcagaacg ggccggattt caggttaccg tgggaggtta
     9241 aaatggcata cctggtggca gtaaccgcct gcgtaagcgg cgtggcgcat acttatatgg
     9301 cggcggaacg gctggaaaag ttgtgccagt tagagaagtg gggcgtcagc attgaaactc
     9361 agggcgcgct gggaacggag aatcgtttag cggacgagga tatccgtcgg gcggatgttg
     9421 ctctgttgat tacggatatc gagcttgccg gtgccgagcg atttgaacat tgccgctatg
     9481 tgcaatgcag catctacgca ttcctgcgtg agccgcagcg ggtaatgagc gcggtgcgca
     9541 aagtgctttc tgcgccgcag caaacccatc ttattctgga gtagtcggtt tttctgtcag
     9601 ttggctgtgg tactgccggc gatattccga cggcgagcgt tcggtgtttt tacgaaacag
     9661 acggcagaag tagttgctgt cgacaaagcc gcaggcgtgc gccacttctt ttaccttcag
     9721 gtcatagcct ttcaataacg tcttagcgtg ctccagtcgc gtgtgattca ggtattcgtt
     9781 aaaaccaatg gcccccgttt tttgaaacag gtgcgagaga tagtttggtg aaatatagaa
     9841 tgcctgtgcg acagattcgc gggtaagcgc ggaggcatag cgttcgtcga tataatcgcg
     9901 aatagcttca aatagtgcct ggctgcgtga ggcggtctgg atttggctgc cgagcaaatc
     9961 gcggcagtgg ctaatcaggc tggctacgat aagccttgcc gtttgctgct cctgcggctg
    10021 catttgcatt tcattgagcg tttgtagcag aaaagaacct atacgtgggc cgcgtcgggc
    10081 gacgtgttgc ttcgccagat tttgatattg tttgccatcc cattgcacga cgctgaagcc
    10141 gagttgctgt ttgccaaaca gcacgctaaa ggtggtagcg ggtgttttcc attgtgggaa
    10201 attccagcca ccagcggcaa cgtacagcac atcgccggga actaacgttg ctccagcgcc
    10261 ggtatcaaca aactcacctt ccagcacaat ttccagccgt ggaaaatcga cctgatacgc
    10321 aagatcgggc accgggccgt tactgctggc aaagtaaatc tggcgcaggg acagcgggcc
    10381 gttgatcagg cgggagagca gatagctgac gtcgtgatac atgttattcc cttaggtatg
    10441 tagacgggaa aacagtaact ggcattatta aaaaaggcca cctgaaaagt gtggcctgag
    10501 gggagttcga tgtaaacatt gcggcagtat cgcatccggc aaatagcgcc agacgcgata
    10561 caaaggtcat ttactgatta cccacctgat cgccatacgg cagagtgtcg taatcgatca
    10621 gcgcgttttt cttgtacggg ttaccaatcc agcgggtagt ttctttgaac tgcggattca
    10681 ccactgaacg ggttggatcg taaccgtcgt aagataagcc cgccaaatct gaccaggtgt
    10741 ggatcagttc cgccaggctg tatttacgat cgacatcctg tgagaaatca cggggatgag
    10801 tcgcttgcca tttttccgac gtccacagca ggaacggaat ggtgtacatg tggcgcgtcg
    10861 ggttgtcttc gttacgcccc tgagttttgt gcggcggcgt gtcgtaaacc tcttcaccgt
    10921 ggtcagagaa gtaaaccagg aatccgttcg ggtctgccgc tttaaagtct ttaatcaggc
    10981 tggcaaccac atgatcgtta tacaggttag cgttgtcata atcgttatat gattccagct
    11041 cttctgcgct taatcctggc ggaacatgat cggtattgcc atcaaacttg ccctgatttt
    11101 ccgggtagcg gtatttgtat ttgatatgcg tacccagcag atgaacgatg atcagtttct
    11161 tcggcgcagg gtcattcagc acatcctgga acggcttcag cacgttggtg tcgtattcac
    11221 gcgcactctg cgtacgttgc tggttcatgt agtactgctt gtcggtctgg cgcgaaaata
    11281 ccgtcagcat ggtattgcgg gcggtcatcg tctgctggtt ggtgatccag aaggttttat
    11341 aacccgcctg tttcatcatg ttcatcagcg acggctgcgt cagatacaga tccgggttct
    11401 tttcattggc aaaggtcagc gcctgttgca ggatttcaat ggtgtacgga cgagacgtaa
    11461 ctacgttatt aaacacggtc agattcggat cggttttatg cagcgcatcc agctccggcg
    11521 tggtttcacg cggataaccg tacagactca tgcgtccgcg ctgggtcgac tcgccaatca
    11581 ccagcactaa agtgcgcggt tcgttacccg attcatcttt gaaattagcc agtggcggca
    11641 aggcattatt ttcattcagt aactttgtta gcgagtttag ttgctgacga tactgataat
    11701 agccggtcag gaattgccac ggtgcggcag gctccatacg cgaggccagg ttatccaacg
    11761 ttttctcaaa cggcttgttt ttgataaacg tattcatggc gatcggatgc agaatcaagc
    11821 cataaagcag ggcaaaagag acaacataac gccacggctt tggaatatag accgggcgca
    11881 ggcgtgtcca cagcagaact gccaccgccg tataggccag cgcgataagc acaattttca
    11941 ggctgaaata ctggcttaaa tactcgctgg cttcgttggt gttggtttcg aacatcacaa
    12001 acagaacgct ctgcgagaac tcctgcccgt agatgacgta gtagcatagc gccgccagag
    12061 aggccgccca tagcaccacg ccgattactg cggcaataat tttaatccgc ttcggaaaga
    12121 ggaataccgg gatcaaccac agcgagctga ataacagcga gtcgcgaatg ccgttggtgc
    12181 cactataacc actgatgtaa ataatggcct gtagcagagt agagaaaaac caaaagtaga
    12241 gcagtgccca acccagggct ttccagctaa aaaggggttt agcctggact tctgtggaat
    12301 gcatagtaag aacctgtctt gaaaaaaata tcgccgaatg taacgacaat tccttaagga
    12361 tatctgaagg tatattcaga atttgaataa aatgcagaca gaaatatatt gaaaacgagg
    12421 gtgttagaac agaagtattt cagaaaaccc tcgcgcaaaa gcacgagggt ttgcagaaga
    12481 ggaagattag ccggtattac gcatacctgc cgcaatcccg gcaatagtga ccattaacgc
    12541 ttgttcgacg cgaggatccg gttcctggcc ttctttttct gcctggcggg agcggtgcag
    12601 caactcggcc tgcaatacgt tcagcgggtc ggtgtaaata ttccgtagct gaatagactc
    12661 tgcaatccac ggcagatcgg ccatcagatg ggaatcgttg gcaatcgcca gcaccacttt
    12721 gatgtcttct tcttgcaggt tgcgtaactc tttacctaac ggccacagtg ctttgtctac
    12781 cagacgttga tcatagtatt ccgccagcca caggtctgct ttggcgaaga ccatctccag
    12841 catgccgaga cgcgtcgaga agaatggcca atcgcggcac atggcttcca gctcgctctg
    12901 tttgccatct tcgaccactt tttgcagcgc cgtacctgca cccagccagg cggggagcat
    12961 caggcggttt tgcgtccagg caaaaatcca cggtatggcg cgcagcgact cgacgccgcc
    13021 ggttgggcga cgtttcgccg gacgtgaacc caacggcagt ttgcccaatt cttgttccgg
    13081 cgtagcggag cggaagtaag gcacaaaatc tttgttttca cgtacgtagc cacggtagag
    13141 atcgcaggag atgactgaca gttcatccat aatgcgacgc cagctctctt tcggctcagg
    13201 tggtggcagc aggttggctt ccagaatcgc cccggtgtac agcgacaggc tgctgacggt
    13261 gatttctggc agaccatatt taaagcggat catctcgccc tgttcagtca cgcgcaggcc
    13321 acctttcagg cttcctggcg gttgtgacag cagcgccgca tgagcaggtg cgccgccgcg
    13381 accaatggaa ccgccgcgac cgtggaacaa cgtcagctca atacccgctt tttcgcaggt
    13441 tttgattaat gcatcctgtg cctgatattg cgcccaggaa gctgccatca ctcccgcatc
    13501 ttttgctgag tcggaatagc caatcatcac catctgtttg ccctgaatca ggccacgata
    13561 ccagtcaata ttgagcagct gggtcatgac atcgttggcg ttgttcagat catcgagggt
    13621 ttcaaacagc ggagcaaccg gcatcgcaaa cccgataccc gcttctttca gcagcaggtg
    13681 gacagccagt acgtcggacg gcgttttcgc catcgagatc acgtaggcgg caatggagcc
    13741 ttgcggcgct tcggcaatca cctggcaggt atcgagcact tcgcgcgttt cggcgcttgg
    13801 ttgccagttg cgcggcagaa gcggacgttt ggagttcagt tcgcggatca ggaacgcctg
    13861 tttgtcggcc tctgaccagc tttcgtagtc gccgataccg aggtagcggg tcagctcgcc
    13921 cagcgcttcg gtatggcggg tgctctcctg acggatatca atacggacca gcggtacgcc
    13981 gaaacatttc acgcggcgca gggtgtcgag cagatcgccg ttggcgataa tacccatgcc
    14041 acacgcctga agtgactggt agcaagcgta gagcggttcc cacagttctt cgttttgtgt
    14101 cagcagccct tctggttttg gcagttcttc gcctttcagg cgcgcttcca gccatgcctg
    14161 tgtcgccatc aggcgagaac gcaggttttt catcagatag cgatacggtt ctgcggcacc
    14221 ttcttcgcca accagcgcca gcagttcagg ggtcgcttca accatcgaca gttcagaaac
    14281 cagcacctga atatctttca ggaacaaatc ggtggctttc cagcggctga gtagcaggac
    14341 gtggcgggtg atatcggcag tgacgttcgg gttgccgtcg cggtcgccgc ccatccacga
    14401 agtaaaacgg accggaacaa attcgacggg cagtttgtag ccgaggttct cttccagttg
    14461 ttcgttcagt tcgcgcaggt aatttggtac gccttgccac aggctgtttt ccactacggc
    14521 aaagccccat ttggcttcat ctaccgggct tggacgcagc ttacggattt catcggtatg
    14581 ccatgactgg gcgattaact ggcgcaggcg acgcatcagc tggttgtgtt cgtagtcagc
    14641 gatatctttg ttatcgagct gttttaagca ggcgttcact tccaccattt tgtggatcag
    14701 tgtacgacgg gtaatttcgg tagggtgagc cgtgaggacc agttccagcg acagcgattc
    14761 cactgctttt ttgatggtgt cttcgctcag ttccggctgg tttttcagtt tacgcagggt
    14821 gcgggcgatc acttccgggt tgctggcagc ttcgcctttc ggcgaaatgc tgtggtattg
    14881 ctcggcggtg ttggccaggt tcaggaactg actaaacgca cgcgcaacgg gcagcagctc
    14941 gtcgttcgac aaattttgta aggtggtgag taactcctgg cggttagcat cattgccagc
    15001 gcgtgaagat ttcgacaact tacggatagt ttctacgcgt tcaagaatgt gttctcccaa
    15061 cgcatccttg atggtttctc ccagcacttt gccgagcata ctgacattac tacgcaatgc
    15121 ggaatattgt tcgttcatat taccccagac accccatctt atcgtttgat agccctgtat
    15181 ccttcacgtc gcattggcgc gaatatgctc gggctttgct tttcgtcgtc ttttataaag
    15241 ccacgtaaaa gcggtgacgt caaatgctgc gaaatcgctt cagcaaacga ataaatagca
    15301 ggaatttacg tcattaaatt cacgacgctt taaataagcg taacttatgg aaatgttaaa
    15361 aaatcgcccc aagtaacacc aaaggtgtag gtcggataag atgcgcaagt atcgcatccg
    15421 acattattgc ggcactggag tttgacaaca gtgccggatg cggcgcgagc gccttatccg
    15481 gcctacgttt taatgccagc aaaaatggtg aattacctgg ataatcaact cacgggtggg
    15541 tttaataaac cgcgtctcca gatattcatc aggctgatga gcttgattga tggaacccgg
    15601 accgagcacc agcgtcgggc ataacgtttg aataaacggc gcttcggtac agtaattcac
    15661 cacttcggtt tttgctccga gcaatttctc aaccacttca accagttgat gattcggtgg
    15721 gcattcatag ccagggatcg gcggatgcag ctcgtcgacc gtcagacgac ccggccagcg
    15781 ttcgctcacc ggagccaatg catcgttgag caaaccatta agttcattga gtgtcatgcc
    15841 aggcagcgga cgaatatcca tatgcaactc acagcaagcg caaatacggt tagaagcgtc
    15901 gccaccgtga atatgcccga ggttgagcgt agggtatggc acggtaaatg cttcgtagtg
    15961 ataacgttct ttcaggttat cgcgcaattg caaaatatgc ccgatggcgt cgtgcattag
    16021 ttcgatagcg ttaactccgc gtgctggatc gctggagtgc cccgactggc cctgaatacg
    16081 gatggcgtta gagatatgac ctttatgtgc gcgtaccggt tgtagtgacg tcggttcgcc
    16141 aatgatggcg caatccgggc gcagggcggt agtttcggca aaataacgcg ctccggccat
    16201 actggtttct tcatcagcag ttgccagaat gtagagcggt tttttcagtt tcgtgacgtc
    16261 gacatcgcgt agcgtatcaa ggataaacgc aaaaaagcct ttcatgtcgg cggtgcctaa
    16321 gccgtaaagc ttgccgtcat gctccgtcag tgtaaacgga tcacgcgtcc agcgaccgtc
    16381 atcaaatggt accgtatcgg tatgtcccgc cagcaacaag ccgccagccc cctgtccgca
    16441 actggccagc atattaaatt tgttgcgagt tcctggcaca ggctgcactt ccacattgaa
    16501 gcctaaatct ttaaaccagt ccgccagcag agtgattaaa tctgcattgc tttgatcgag
    16561 tgcctcttcc gtggcgctta ttgaaggtgt ggcaatcaga gcacggtaaa tctcgataaa
    16621 tggcggtaat ttgtttttca ttgttgacac acctctggtc atgatagtat caatattcat
    16681 gcagtattta tgaataaaaa tacactaacg ttgagcgtaa taaaacccac cagccgtaag
    16741 gtgaatgttt tacgtttaac ctggcaacca gacataagaa ggtgaatagc cccgatgttg
    16801 aatacgctga ttgtgggtgc cagcggctac gctggcgcag agctagtgac ctatgtaaat
    16861 cgccatccgc atatgaacat aaccgctttg actgtttcag cgcaaagcaa tgatgcggga
    16921 aagttaatct ccgatttgca tccgcagcta aaaggcatcg ttgagctgcc gttgcagccg
    16981 atgtcggata tcagcgagtt tagcccaggg gtggacgtgg tgtttctcgc caccgcccat
    17041 gaagttagcc acgatttagc gccgcagttt cttgaagcgg gctgcgtggt gttcgacctt
    17101 tccggcgcgt ttcgtgttaa cgacgccacc ttctatgaaa aatattacgg ctttacccat
    17161 caatacccgg aactgttgga acaggcagcc tacggtctgg cggagtggtg cggtaataaa
    17221 ttaaaagaag cgaatttgat tgcggtgccg ggctgttatc cgacggcggc acagctggcg
    17281 ctgaaaccgt tgattgatgc cgatcttctt gacctcaatc agtggccggt gatcaacgcc
    17341 accagcggcg tgagcggtgc agggcgtaaa gcggccattt caaacagctt ttgtgaagtt
    17401 agcctgcaac cgtatggcgt ctttactcat cgccatcaac cagagatcgc cacacacctc
    17461 ggtgctgacg ttatcttcac cccacatctg ggcaatttcc cgcgcggcat tctcgaaacc
    17521 attacctgcc gcctgaaatc gggtgtgacc caggcgcaag tcgcgcaagt gttacagcag
    17581 gcgtatgccc ataaaccgct ggtgcggctg tatgacaaag gcgttccggc gctgaaaaat
    17641 gtcgttgggc tgccattttg cgatatcggg tttgccgttc agggcgagca tctgattatt
    17701 gtggcgaccg aagacaactt actgaaaggc gcggcggcac aagcggtaca gtgcgccaat
    17761 attcgtttcg gctatgcgga aacgcagtct cttatttaag ggtgcaatga tgaatccatt
    17821 aattatcaaa ctgggcggcg tactgctgga tagtgaagag gcgctggaac gtctgtttag
    17881 cgcactggtg aattatcgtg agtcacatca gcgtccgctg gtgattgtgc acggcggcgg
    17941 ttgcgtggtg gatgagctga tgaaagggct gaatctgccg gtgaaaaaga aaaacggcct
    18001 gcgggtgacg cctgctgatc agatagacat tatcaccgga gcactggcgg gaacggcaaa
    18061 taaaaccctg ttggcatggg cgaagaaaca tcagattgcg gccgtaggtt tgtttctcgg
    18121 tgacggcgac agcgtcaaag tgacccagct tgatgaagag ttaggtcatg ttggactggc
    18181 gcagccaggt tcgcctaagc ttatcaactc cttgctggag aacggttatc tgccggtggt
    18241 cagctccatt ggcgtaacag acgaagggca actgatgaac gtcaatgccg accaggcggc
    18301 aacggcgctg gcggcaacgc tgggcgcgga tctgattttg ctctccgacg tcagcggcat
    18361 tctcgacggc aaagggcaac gcattgccga aatgaccgcc gcgaaagcag aacaactgat
    18421 tgagcagggc attattactg acggcatgat agtgaaagtg aacgcggcgc tggatgcggc
    18481 ccgcacgctg ggccgtccgg tagatatcgc ctcctggcgt catgcggagc agcttccggc
    18541 actgtttaac ggtatgccga tgggtacgcg gattttagct taagttttgt tggccggagg
    18601 cgcagctttc cggcattgaa tttcaaaata aggaaacaga gttatggcac tttggggcgg
    18661 gcgttttacc caggcagcag atcaacggtt caaacaattc aacgactcac tgcgctttga
    18721 ttaccgtctg gcggagcagg atattgttgg ctctgtggcc tggtccaaag ccctggtcac
    18781 ggtaggcgtg ttaaccgcag aagagcaggc gcaactggaa gaggcgctga acgtgctgct
    18841 ggaagatgtt cgcgccaggc cacaacaaat ccttgaaagc gacgccgaag atatccatag
    18901 ctgggtggaa ggcaaactga tcgacaaagt gggccagtta ggcaaaaagc tgcataccgg
    18961 gcgtagtcgt aacgatcagg tagcgactga cctgaaactg tggtgcaaag ataccgttag
    19021 cgagttactg acggctaacc gacagctgca atcggcgctg gtggaaaccg cacaaaacaa
    19081 tcaggacgcg gtcatgccag gttacactca cctgcaacgc gcccagccgg tgacgttcgc
    19141 gcactggtgc ctggcctatg ttgagatgct ggcgcgtgat gaaagccgtt tgcaggatgc
    19201 gcttaagcgt ctggatgtca gcccattagg ctgtggcgcg ctggcgggaa cggcctatga
    19261 aatcgaccgt gaacagttag caggctggct gggctttgct tcggcgaccc gtaacagtct
    19321 cgacagcgtt tctgaccgtg accatgtgtt ggaactgctt tctgctgccg ctatcggcat
    19381 ggtgcatctg tcgcgttttg ctgaagatct gattttcttt aacaccggcg aagcggggtt
    19441 tgtggagctt tctgaccgcg tgacttccgg ttcatcatta atgccgcaga agaaaaaccc
    19501 ggatgcgctg gagctgattc gcggtaaatg cggtcgggtg cagggggcgt taaccggcat
    19561 gatgatgacg ctgaaaggtt tgccgctggc ttacaacaaa gatatgcagg aagacaaaga
    19621 aggtctgttc gacgcgctcg atacctggct ggactgcctg catatggcgg cgctggtgct
    19681 ggacggcatt caggtgaaac gtccacgttg ccaggaagcg gctcagcagg gttacgccaa
    19741 cgccaccgaa ctggcggatt atctggtggc gaaaggcgta ccgttccgcg aggcgcacca
    19801 tattgttggt gaagcggtgg tggaagccat tcgtcagggc aaaccgctgg aagatctgcc
    19861 gctcgacgag ttgcagaaat tcagcccggt gattgacgaa gatgtctatc cgattctgtc
    19921 gctgcaatcg tgcctcgaca agcgtgcggc aaaaggcggc gtctcaccgc agcaggtggc
    19981 gcaggcaatt gcttttgcgc aggcgcggtt ggggtaagaa catttatatg tataaatttg
    20041 agcctggctt atcgccgggc ttttttatgg caaaaaaaag cggatcctgg agatccgcaa
    20101 aagttcacgt tggctttagt tattcgagtt gagaaactct cgaaacgggc agtgacttca
    20161 agggttaaaa gaggtgccgc tccgtttctg tgagcaatta tcagtcagaa tgcttgatag
    20221 ggataatcgt tcattgctat tctacctatc gccatgaact atcgtggcaa tggaggatgg
    20281 ataatgaata ttcgtgatct tgagtacctg gtggcattgg ccgaacaccg ccattttcgg
    20341 cgtgcggcag attcctgcca cgttagccag ccgacgctta gcgggcaaat tcgtaagctg
    20401 gaagatgagt tgggcgtgat gttgctggag cggaccagcc gtaaagtgtt gttcacccag
    20461 gcgggaatgc tgctggtaga tcaggcgcgt accgtactgc gtgaggtgaa agtccttaaa
    20521 gagatggcaa gccagcaggg cgagacgatg tccgggccgc tgcacattgg tttgattccc
    20581 acagttggac cgtacctgct accgcatatt atccctatgc tgcaccagac ctttccaaag
    20641 ctggaaatgt atctgcatga agcacagacc caccagttac tggcgcaact ggacagcggc
    20701 aaactcgatt gcgtgatcct cgcgctggtg aaagagagcg aagcattcat tgaagtgccg
    20761 ttgtttgatg agccaatgtt gctggctatc tatgaagatc acccgtgggc gaaccgcgaa
    20821 tgcgtaccga tggccgatct ggcaggggaa aaactgctga tgctggaaga tggtcactgt
    20881 ttgcgcgatc aggcaatggg tttctgtttc gaagccgggg cggatgaaga tacacacttc
    20941 cgcgcgacca gcctggagac gctgcgcaac atggtggcgg caggtagcgg gatcacttta
    21001 ctgccagcgc tggctgtgcc gccggagcgc aaacgcgatg gggttgttta tctgccgtgc
    21061 attaagccgg aaccacgccg cactattggc ctggtttatc gtcctggctc accgctgcgc
    21121 agccgctatg agcagctggc agaggccatc cgcgcacgaa tggatggcca tttcgataaa
    21181 gtgttaaaac aggcggttta aaccgtttaa cgcagctacc cgataggctt ccgccatggt
    21241 cgggtagtta aaggtggtgt tgacgaagta ctcaatagtg ttgccgccac ctttctgttc
    21301 cataatcgcc tgaccgatat gaataatttc ggcagcgcgc tcgccaaagc agtgaatacc
    21361 cagaatctct tttgtttccc gatggaacaa aattttcagc gtgcccacgt tcatgccgac
    21421 gatttgtgcg cgtgccagat gtttaaactg ggcgcggccc acttcatatg gcactttcat
    21481 cgcggtcagc tgctgttcgg ttttgcccac agagctgatt tccgggatgg tgtaaatgcc
    21541 ggtagggata tcttcaatca gatgcgcggt ggcttcgcct tttaccagcg cctgcgcggc
    21601 aatgcgcccc tggtcatagg ccgctgatgc caggctcgga taaccaatca cgtcgcccac
    21661 cgcgtaaacg tgcggctgtg cggtctgata catgctgttg accttcagct gtccgcgact
    21721 gtcagtttcc agcccaatgt tctgtaacgc cagcgaatcg gtattaccgg tgcgaccgtt
    21781 ggcatagagc aggcagtcag ctttcagttt tttacccgac ttcaaatgca tgatcacacc
    21841 atcgtcacag ccttcgatct tctcgtactc ttcgttgtga cgaatcacta cgccactgtt
    21901 ccagaagtga taggagagag aatctgacat ctcttgatcg agaaatgcca gcaggcgatc
    21961 gcgggtgttg atcagatcca cttttacatc cataccgcgg aagatcgacg catattcaca
    22021 gccgatcact ccagcaccat agataagtac atggcgcggt tcgtggtgca tgctaagaat
    22081 tgagtcgctg tcgtaaatgc gtggatgggt gaaatcaaca tctgttggat gatatggacg
    22141 agagccgcag gcaataacaa atttttcagc ggttagtgtt tcgacgctgc cgtccgggca
    22201 attcagcgcc aacgtatgct cgtcaacaaa gcgagcgttc ccctgcaata tttcacagtg
    22261 attacgttcg taaaatccct gacgcatgcg cgtttgttga ttaatcacgt tatcggcatg
    22321 gttaaggata tcggcaaaag aagagcggag cagtcgggaa tggtcgctgt atagtgggtt
    22381 ttgattgaat tctataatgc ggctgacggc gtgacggaga gctttcgacg ggatggtgcc
    22441 ccagtgggtg caaccgccgc caacattttg ataacgctcg ataactgcga cgcgcgcacc
    22501 ttgcttaacc aggcccattg cagcgccttc gccgccgggg ccggaaccta ttactatggc
    22561 atcgtaatcg taggaatgtg gcatggtagg gcttacctgt tcttatacat agaagcaaca
    22621 gaatggtaac attttatcgc gggtaagcca attgatcccc gtcatttatc tggctatatc
    22681 ctgagcggcc tttgctttgt ctgtttctta cttttaccct gacgttttat tggattttta
    22741 tcgacgatac tctccgttta agcggcaggt ttccgctgta cgtaaaagaa ccggccaaag
    22801 aattgcagta aatatgtttt attgcgttac cgttcattca caatactgga gcaatccagt
    22861 atgttcattc tctggtatag tgccagcagt acttttggca aggattcaga catcgtgatg
    22921 ggcgtaagag cgcaacaaaa agaaaaaacc cgccgttcgc tggtggaagc cgcatttagc
    22981 caattaagtg ctgaacgcag cttcgccagc ctgagtttgc gtgaagtggc gcgtgaagcg
    23041 ggcattgctc ccacctcttt ttatcggcat ttccgcgacg tagacgaact gggtctgacc
    23101 atggttgatg agagcggttt aatgctacgc caactcatgc gccaggcgcg tcagcgtatc
    23161 gccaaaggcg ggagtgtgat ccgcacctcg gtctccacat ttatggagtt catcggtaat
    23221 aatcctaacg ccttccggtt attattgcgg gaacgctccg gcacctccgc tgcgtttcgt
    23281 gccgccgttg cgcgtgaaat tcagcacttc attgcggaac ttgcggacta tctggaactc
    23341 gaaaaccata tgccgcgtgc gtttactgaa gcgcaagccg aagcaatggt gacaattgtc
    23401 ttcagtgcgg gtgccgaggc gttggacgtc ggcgtcgaac aacgtcggca attagaagag
    23461 cgactggtac tgcaactgcg aatgatttcg aaaggggctt attactggta tcgccgtgaa
    23521 caagagaaaa ccgcaattat tccgggaaat gtgaaggacg agtaatgaaa caagcaaatc
    23581 aagatagagg tacgctgctg ctggcgttag ttgctggctt atcgattaat ggtactttcg
    23641 cagcgctgtt tagctccatt gtgccatttt ctgtattccc gattatttcc ctggtgctga
    23701 cggtttactg cctgcatcaa cgttatctta atcgcactat gccggtaggc ttgccgggtc
    23761 tggcagctgc ctgttttatt ctcggcgtac tgctgtacag cacggtagtt cgtgcggaat
    23821 atccggatat cggctctaac ttcttcccgg cagtactctc cgtcattatg gtgttctgga
    23881 ttggcgcgaa gatgcgtaac cgtaagcagg aagttgctga gtaatcggta ttatgccgga
    23941 tgctggcgca tccggcatgg gttttacttc gcggtcagta atacgccgca ctccatatgg
    24001 tgcgtgtagg ggaactgatc aaacagagcc agacgttcga ccttgtgcgt ctggcttaat
    24061 gtttccagat tcttgcataa cgtttccggg ttacaggaga tgtacaaaat acgcggatac
    24121 gcctgcacca ttttctcggt ttcactgtcc agaccgctgc gcggtgggtc gacaaaaatg
    24181 gtttcgcact gataactctt taagtcgatc ccttgcaggc ggttaaactc gcgcacacca
    24241 ttcatcgcct gagtaaattc ttctgccgcc atacgaataa tttgtacgtt atcaatatgg
    24301 ttagctgcga tgttgtattg cgcagcagca accgacggct tagcgatttc ggtggctaat
    24361 acccgatcaa aattgcgcgc cagcgctaat gaaaagttac cgttgccgca gtacagctcc
    24421 agtaaatcgc cttttgagcc tttggttacg tccagcgccc attccagcat ctgaatattc
    24481 atcgccgcgt tcggctgggt aaagctgttt tctacctgac ggtagatcat ctctttccct
    24541 gcgaccggca gacgctcgtc gatgtaatct tgatccagct cgattttggt tttcgttgcc
    24601 cgaccaatca gatgcacatt cagattctgc gcgcgcagtg catcgcgcag ggcctccgct
    24661 tcctgacgcc actcatcatc cagcttctta tggtatagca gggaaaccac cgcctgatta
    24721 ctcagcgtgg tgaggtaatc aatctggaac aacttgtggc gcagaacggg attattacgc
    24781 acacccgcaa tcatcgccgt catcaactgg ttgataagtt cactggcggc ggggaagcta
    24841 tccacgcgga tacggctttt ggtttgttga tcgaaaatga tgtgatacag gtcatcgcca
    24901 tcgtgccaga tgcggaactc cgcacgcatc cggtaatgac tgaccggcga gcgaaacact
    24961 tccggaacca ggtcagaaaa cggtgccatc atactttgca aacgtaccac tttttcggct
    25021 aactgcgctt catactgttc tgttggaagg tgttcggggg tcatgatgta tcctgaaaaa
    25081 ttaaagtacg cggcgattgt agggattgct catcagatgt ccagatcttg atgaattcct
    25141 atttgtgagc tacgtctgga cagtaacttg ttacaacctg tagcatccac ttgccggtcc
    25201 tgtgagttaa tagggaatcc agtgcgaatc tggagctgac gcgcagcggt aaggaaaggt
    25261 gcgatgattg cgttatgcgg acactgccat ccggtgggaa gtcatcatct cttagtatct
    25321 tagatacccc tccaagcccg aagacctgcc ggccaacgtc gcatctggtt ctcatcatcg
    25381 cgtaatattg atgaaacctg cggcatcctt cttctattgt ggatgcttta caatgattaa
    25441 aaaagcttcg ctgctgacgg cgtgttctgt cacagccttt tccgcttggg cacaggatac
    25501 cagcccggat actctcgtcg ttactgctaa ccgttttgaa cagccgcgca gcactgtgct
    25561 tgcaccaacc accgttgtga cccgtcagga tatcgaccgc tggcagtcga cctcggtcaa
    25621 tgatgtgctg cgccgtcttc cgggcgtcga tatcacccaa aacggcggtt caggtcagct
    25681 ctcatctatt tttattcgcg gtacaaatgc cagtcatgtg ttggtgttaa ttgatggcgt
    25741 acgcctgaat ctggcggggg tgagtggttc tgccgacctt agccagttcc ctattgcgct
    25801 tgtccagcgt gttgaatata tccgtgggcc acgctccgcc gtttatggtt ccgatgcaat
    25861 aggtggggtg gtgaatatca tcacgacgcg cgatgaaccc ggaacggaaa tttcagcagg
    25921 gtggggaagc aatagttatc agaactatga tgtctctacg cagcaacaac tgggggataa
    25981 gacacgagta acgttgttgg gcgattatgc ccatactcat ggttatgatg ttgttgccta
    26041 tggtaatacc ggaacgcaag cgcagacaga taacgatggt tttttaagta aaacgcttta
    26101 tggcgcgctg gagcataact ttactgatgc ctggagcggc tttgtgcgcg gctatggcta
    26161 tgataaccgt accaattatg acgcgtatta ctctccgggt tcaccattgg tcgatacccg
    26221 taaactctat agtcaaagtt gggacgccgg gctgcgatat aacggcgaac tgattaaatc
    26281 acaactcatt accagctata gccatagcaa agattacaac tacgatcccc attatggtcg
    26341 ttatgattcg tcggcgacgc tcgatgagat gaagcaatac accgtccagt gggcaaacaa
    26401 tgtcatcgtt ggtcacggta gtattggtgc gggtgtcgac tggcagaaac agactacgac
    26461 gccgggtaca ggttatgttg aggatggata tgatcaacgt aataccggca tctatctgac
    26521 cgggctgcaa caagtcggcg attttacctt tgaaggcgca gcacgcagcg acgataactc
    26581 acagtttggt cgtcatggaa cctggcaaac cagcgccggt tgggaattca tcgaaggtta
    26641 tcgcttcatt gcttcctacg ggacatctta taaggcacca aatctggggc aactgtatgg
    26701 cttctacgga aatccgaatc tggacccgga gaaaagcaaa cagtgggaag gcgcgtttga
    26761 aggcttaacc gctggggtga actggcgtat ttccggatat cgtaacgatg tcagtgactt
    26821 gatcgattat gatgatcaca ccctgaaata ttacaacgaa gggaaagcgc ggattaaggg
    26881 cgtagaggcg accgccaatt ttgataccgg accactgacg catactgtga gttatgatta
    26941 tgtcgatgcg cgcaatgcaa ttaccgacac gccgttgtta cgccgtgcta aacagcaggt
    27001 gaaataccag ctcgactggc agttgtatga cttcgactgg ggtattactt atcagtattt
    27061 aggcactcgc tatgataagg attactcatc ttatccttat caaaccgtta aaatgggcgg
    27121 tgtgagcttg tgggatcttg cggttgcgta tccggtcacc tctcatctga cagttcgtgg
    27181 taaaatagcc aacctgttcg acaaagatta tgagacagtc tatggctacc aaactgcagg
    27241 acgggaatac accttgtctg gcagctacac cttctgaacc acgtcccacc gtgctggtgt
    27301 ttgactccgg cgtcggtggg ttgtcggtct atgacgagat ccggcatctc ttaccggatc
    27361 tccattacat ttatgctttc gataacgtcg ctttcccgta tggcgaaaaa agcgaagcgt
    27421 ttattgttga gcgagtggtg gcaattgtca ccgcggtgca agaacgttat ccccttgcgc
    27481 tggctgtggt cgcttgcaac actgccagta ccgtttcact tcctgcatta cgcgaaaagt
    27541 tcgacttccc ggttgttggt gtcgtgccgg cgattaaacc tgctgcacgt ctgacggcaa
    27601 atggcattgt cggattactg gcaacccgcg gaacagttaa acgttcttat actcatgagc
    27661 tgatcgcgcg tttcgctaat gaatgccaga tagaaatgct gggctcggca gagatggttg
    27721 agttggctga agcgaagcta catggcgaag atgtttctct ggatgcacta aaacgtatcc
    27781 tacgcccgtg gttaagaatg aaagagccgc cagataccgt tgtattgggt tgcacccatt
    27841 tccctctact acaagaagaa ctgttacaag tgctgccaga gggaacccgg ctggtggatt
    27901 ctggcgcagc gattgctcgc cgaacggcct ggttgttaga acatgaagcc ccggatgcaa
    27961 aatctgccga tgcgaatatt gccttttgta tggcaatgac gccagaagct gaacaattat
    28021 tgcccgtttt acagcgttac ggcttcgaaa cgctcgaaaa actggcagtt ttaggctgat
    28081 ttggttgaat gttgcgcggt cagaaaatta ttttaaattt cctcttgtca ggccggaata
    28141 actccctata atgcgccacc actgacacgg aacaacggca aacacgccgc cgggtcagcg
    28201 gggttctcct gagaacttcg gcagagaaag caaaaa
//
LOCUS       DAZKIV010000047        28198 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZKIV010000047 DAZKIV010000000
VERSION     DAZKIV010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.047"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(49..1413)
                     /gene="mnmE"
                     /locus_tag="ACHOT6_002014"
     CDS             complement(49..1413)
                     /gene="mnmE"
                     /locus_tag="ACHOT6_002014"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFP9947237.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1519..3165)
                     /gene="yidC"
                     /locus_tag="ACHOT6_002015"
     CDS             complement(1519..3165)
                     /gene="yidC"
                     /locus_tag="ACHOT6_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFP9947238.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3168..3425)
                     /gene="yidD"
                     /locus_tag="ACHOT6_002016"
     CDS             complement(3168..3425)
                     /gene="yidD"
                     /locus_tag="ACHOT6_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFP9947239.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3389..3748)
                     /gene="rnpA"
                     /locus_tag="ACHOT6_002017"
     CDS             complement(3389..3748)
                     /gene="rnpA"
                     /locus_tag="ACHOT6_002017"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFP9947240.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3765..3905)
                     /gene="rpmH"
                     /locus_tag="ACHOT6_002018"
     CDS             complement(3765..3905)
                     /gene="rpmH"
                     /locus_tag="ACHOT6_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFP9947241.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4135..4215)
                     /locus_tag="ACHOT6_002019"
     CDS             complement(4135..4215)
                     /locus_tag="ACHOT6_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947242.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4512..5915
                     /gene="dnaA"
                     /locus_tag="ACHOT6_002020"
     CDS             4512..5915
                     /gene="dnaA"
                     /locus_tag="ACHOT6_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFP9947243.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            5920..7020
                     /gene="dnaN"
                     /locus_tag="ACHOT6_002021"
     CDS             5920..7020
                     /gene="dnaN"
                     /locus_tag="ACHOT6_002021"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFP9947244.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7020..8093
                     /gene="recF"
                     /locus_tag="ACHOT6_002022"
     CDS             7020..8093
                     /gene="recF"
                     /locus_tag="ACHOT6_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFP9947245.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8122..10536
                     /gene="gyrB"
                     /locus_tag="ACHOT6_002023"
     CDS             8122..10536
                     /gene="gyrB"
                     /locus_tag="ACHOT6_002023"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFP9947246.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10776..11174
                     /gene="yidB"
                     /locus_tag="ACHOT6_002024"
     CDS             10776..11174
                     /gene="yidB"
                     /locus_tag="ACHOT6_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFP9947247.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11289..12101
                     /gene="yidA"
                     /locus_tag="ACHOT6_002025"
     CDS             11289..12101
                     /gene="yidA"
                     /locus_tag="ACHOT6_002025"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFP9947248.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12147..12803)
                     /gene="yidX"
                     /locus_tag="ACHOT6_002026"
     CDS             complement(12147..12803)
                     /gene="yidX"
                     /locus_tag="ACHOT6_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFP9947249.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPSGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13081..13770
                     /gene="dgoR"
                     /locus_tag="ACHOT6_002027"
     CDS             13081..13770
                     /gene="dgoR"
                     /locus_tag="ACHOT6_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFP9947250.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13767..14645
                     /gene="dgoK"
                     /locus_tag="ACHOT6_002028"
     CDS             13767..14645
                     /gene="dgoK"
                     /locus_tag="ACHOT6_002028"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFP9947251.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14629..15246
                     /gene="dgoA"
                     /locus_tag="ACHOT6_002029"
     CDS             14629..15246
                     /gene="dgoA"
                     /locus_tag="ACHOT6_002029"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFP9947252.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15243..16391
                     /gene="dgoD"
                     /locus_tag="ACHOT6_002030"
     CDS             15243..16391
                     /gene="dgoD"
                     /locus_tag="ACHOT6_002030"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFP9947253.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16466..17803
                     /gene="dgoT"
                     /locus_tag="ACHOT6_002031"
     CDS             16466..17803
                     /gene="dgoT"
                     /locus_tag="ACHOT6_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9947254.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17800..18864)
                     /gene="cbrA"
                     /locus_tag="ACHOT6_002032"
     CDS             complement(17800..18864)
                     /gene="cbrA"
                     /locus_tag="ACHOT6_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFP9947255.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHLCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            18965..20179
                     /gene="yidR"
                     /locus_tag="ACHOT6_002033"
     CDS             18965..20179
                     /gene="yidR"
                     /locus_tag="ACHOT6_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFP9947256.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHLMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20181..20513)
                     /gene="yidQ"
                     /locus_tag="ACHOT6_002034"
     CDS             complement(20181..20513)
                     /gene="yidQ"
                     /locus_tag="ACHOT6_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9947257.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20753..20825
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20819..21232
                     /gene="ibpA"
                     /locus_tag="ACHOT6_002035"
     CDS             20819..21232
                     /gene="ibpA"
                     /locus_tag="ACHOT6_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFP9947258.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21344..21772
                     /gene="ibpB"
                     /locus_tag="ACHOT6_002036"
     CDS             21344..21772
                     /gene="ibpB"
                     /locus_tag="ACHOT6_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFP9947259.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            21968..23629
                     /gene="yidE"
                     /locus_tag="ACHOT6_002037"
     CDS             21968..23629
                     /gene="yidE"
                     /locus_tag="ACHOT6_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFP9947260.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVVQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23626..24342)
                     /gene="yidP"
                     /locus_tag="ACHOT6_002038"
     CDS             complement(23626..24342)
                     /gene="yidP"
                     /locus_tag="ACHOT6_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9947261.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMQEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24637..26253
                     /locus_tag="ACHOT6_002039"
     CDS             24637..26253
                     /locus_tag="ACHOT6_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9947262.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            26253..27575
                     /locus_tag="ACHOT6_002040"
     CDS             26253..27575
                     /locus_tag="ACHOT6_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
                     /protein_id="HFP9947263.1"
                     /translation="MTKFSVVVAGGGSTFTPGIVLMLLANQDRFPLRALKFYDNDGAR
                     QEVIAEASKVILKEKAPDIAFSYTTDPEVAFSDVDFVMAHIRVGKYPMRELDEKIPLR
                     HGVVGQETCGPGGIAYGMRSIGGVLELVDYMEKYSPNAWMLNYSNPATIVAEATRRLR
                     PNAKILNICDMPIGIESRMAQIVGLQDRKQMRVRYYGLNHFGWWTSIEDLQGNDLMPQ
                     LRQYVSKHGYVPPLQDTHTEASWNDTYAKARDVQALDPDTLPNTYLKYYLFPDYVVQH
                     SNPEHTRANEVMEHREKQVFDACRAITAAGNSAAGKLEIDEHASYIVDLAAAIAFNTQ
                     ERMLLIVPNNGAIHNFDDEAMVEIPCLVGHNGPEPLVVGDIPQFQKGLMSQQVAVEKL
                     VVDAWEQRSYQHLWQAITLSKTVPSASVAKAILDELLEANKAYWPELR"
     gene            complement(27572..27646)
                     /locus_tag="ACHOT6_002041"
                     /pseudo
     CDS             complement(27572..27646)
                     /locus_tag="ACHOT6_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27712..28137)
                     /gene="tnpA"
                     /locus_tag="ACHOT6_002042"
                     /pseudo
     CDS             complement(27712..28137)
                     /gene="tnpA"
                     /locus_tag="ACHOT6_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         6588 a         7192 c         7613 g         6805 t
ORIGIN      
        1 tgaagcgaag ccgcatctga cagtcagaat acggcttcgt aagcgcggtt acttaccaat
       61 acagaagctg gagaaaatcc gccccagcag gtcgtctgaa gtaaattccc cggtgatttc
      121 gcttaagttc tgctgtgcca gacgcaactc ttccgccagc agttcacctg cccaggctcc
      181 caacagttgc gctttgccct gttgtagatg ttccgctgcc tgttccagcg cctgtaggtg
      241 gcgacgacgc gccaggaagc cgccttccat gttggtgtca aagcccatgc tctgtttgag
      301 atggttacgc agcacgtcca cgccttcacc agtccttgcc gagagacgaa ttaacgcgtg
      361 accgttcact tcactcattc ccagcgtttc gccggtgata tcggctttat tgcgcaccac
      421 ggtgatcggc agtttcgctg gcagacgggc aataaattcc ggccagatct ctgccggatc
      481 cacggcgtct gttgtggtgc catcgaccat aaacagcacg cggtcggcct gttcaatttc
      541 ctgccacgcg cgctcgatac caatacgttc cacttcgtca ctggcttcac gtagcccagc
      601 ggtatcgatg atatgcagcg gcattccgtc aatgtggata tgctcacgca gcacgtcacg
      661 tgtggttccg gcgatatcag ttacgattgc tgcttcacgg cccgccagcg cgtttaacag
      721 gctcgattta ccggcgttag gacgtccggc aatcaccacc ttcatccctt cgcgcaacaa
      781 actaccctga cgtgcttcag cacgcactgc atcgagatcg gcaatcacgt cattaagctg
      841 ggcttcaatt ttgccgtcgg agaggaaatc gatctcttca tcagggaaat caattgccgc
      901 ttcgacgtaa atgcgcaagt gggtgagggc ttccaccaga tggttgaccc gtgcggagaa
      961 tgcgccttgc agcgagttaa gtgccgaacg ggccgcctgt tccgaactgg cgtcgataag
     1021 atcggcaatc gcctcggcct gggctaagtc gagtttatcg ttaagaaacg cgcgttcgga
     1081 aaactcacca gggcgagcaa tccgcaggcc gggaatggtc agaatgcgtt ttaacagcag
     1141 gtcgaggatc accggaccgc catgaccttg cagttccagc acatcttcgc cggtgaacga
     1201 gttcgggcca gggaaccata gcgcaatccc ctgatcgagc acgctgccgt cggcgtcttt
     1261 aaacggaaga taatcggcgt agcgcggctt aggcagttta cccagcacgg tttcggcaac
     1321 ttcacgggct ttgaggccgg agatgcgcag gatgccaaca ccgccacgtc ccggaggcgt
     1381 ggcctgggct acgatagtgt cattatcgct catgatgttc ctgttgcttt gtgtggcgga
     1441 tgcgcggtgc ttatccgccc tacgaaaaga aaaaaggcgg tcaactgacc gcccttattt
     1501 tagcgaaaac tcaccgaatc aggatttttt cttctcgcgg ctatgcaggc cacgtttttc
     1561 cagaccacgg taaatcagct gctgctgaat aatggttacc aggttgctga cgatatagta
     1621 cagcaccaga cctgacggga accacaggaa gaacacggtg aagatgaccg gcataaaggt
     1681 catgatcttc tgctgcatcg ggtcggtcac tgtggtcggc gacatcttct gaatgaagaa
     1741 catcgttacg cccatcagga tcggcaggat gtagtacggg tcctgtgccg acaggtcgtg
     1801 gatccacagt gcaaacggtg cctgacgcag ttcaacggaa cccatcagca tgtagtacaa
     1861 cgccaggaag attggcatct ggatcagcag cgggaagcag ccgcccagcg ggttaacctt
     1921 ctcagctttg tacagcgcca tcatttcctg gctgatacgc tgtttgtcat cgcccagacg
     1981 ctcacgcatt gcctgaatct tcggctgcag catacgcatc ttcgccatgg aggtgtactg
     2041 cgctttggtc agcgggtaca tgatgccacg aacgataaag gtgatgatga taatggagaa
     2101 gccccagtta cccacaaagc tatggatcca tttcagcagt ttgaacagcg gctgagagat
     2161 gaaccacaac caaccgtaat caacggtcag atccaggtgc ggagcaacag ctgccatttt
     2221 gtcctggatt tccgggccaa cccacagggt gctgttcatc gcgccagtct gaccaggctg
     2281 aaccagtacc ggctgagatt tatagccgat agcggcgatg ccgttaccca gattagcggt
     2341 atagaagttg ttggtaccgt cgttatgcgg gatccacgcc gtcgcgaaat actgttgcag
     2401 catcgccacc caaccacctt tcgaagagat gttcaggttt tcgttatcgg caatggtatc
     2461 gaacttgtat ttctcatact tctcgtcagg cgtggagtac gccgcgccac ggaaggtgtg
     2521 cagtgcgaag ttgctgcttc cggtatcgag atgcggtggc agagtgatgg attgcttcaa
     2581 ctgaccaaag gtggagattt ccagcggttt ctcgccagcg ttctgcacgt tgtagttgac
     2641 gttgacagcg taatcaccac gtttcaggac aaacgttttg gtaaacgtgt tgcctgccgc
     2701 gtcggtatac gtcatcggca cctgcagttc gttttgacct tcagccagca cataagcgtc
     2761 tttttcaacg ttatacagcg gacgcgggcc gttagccggg ttatccgggc catcacgacc
     2821 ggtcagaccg ctctgtgcct gataaataaa ctgcggtgaa gtttccagca gctggaacgg
     2881 ctgggtagag ttcagctctt tcgggtaagc cggcagcaga gcttgctcaa catcaccacc
     2941 acgggtgttg atggtcagat caagcacgtc ggtcttaacc gagatcagtt tcccctggcc
     3001 actggccggt acgccctggt cggcggcgct acccgctgcg gtggtcgttg tctgcgtggt
     3061 ctgttgggcc tgaggttgcg ggtttttatc ctgctcccag gcttgccaga tcatgaaaga
     3121 cacgaacagc aaagcgatga ctaaaagatt gcgttgcgaa tccatcgtta gtgttctctg
     3181 gtatcaaatg gtccgggcgg gacgggatcg tcaccaccag ggtgtaaagg gtggcatttt
     3241 aatacgcgtt tcaccgtcaa ccaactgcct tttatcactc caaacctgcg caatgcctca
     3301 attccgtagc ttgaacaggt tggagtgaaa cgacaatgcg gcccgagtag cggactaatc
     3361 aggcgttgat agacccgaat gagggctatc aggacccgcg agccaggcga cagtggcggc
     3421 gccataattt ttccaacgct tccgagagag cacggttatc gaggtcggca accccttttt
     3481 tcgccaccac cacgaaatcc atagccggga gttcatgttg gcgcagacgg aagctttcac
     3541 gcgtcagacg tttaatccga ttgcgttcat gggcgcgtcg aacgtttttc ttggcgactg
     3601 taagaccgat acggggatgc cccagcgaat tcaggcggcc gagaatggta atttgcggcg
     3661 tgccagcccg ttgtggctgc tggaagacga atgtgaattg actgggagtt aacaagcgta
     3721 actccctggg aaatgcgagc ttaaccactc aggggttagc tttattactt agaaacggtc
     3781 agacgagcgc ggcctttagc acgacgacgt gccagaacct gacgaccatt tttagtagcc
     3841 atacgagcac ggaagccgtg agaacggttg cgcttcagta cagacggttg aaaagtgcgt
     3901 ttcatggcga tttctaccta aacttgaata aattcaatgg ctttattgga tatccgccga
     3961 aaaatgaaac gatggacacc gaagccatgg gtgattaaag aggccggatt gtaataattg
     4021 tacactccgg agtcaattct ctttccttat ttaccgcgct tttccgcacc ttttcgcagg
     4081 gaaaatgtac gacctcacac cagtggaaac cagcatggcg cgccgggtgg aggattatac
     4141 gggctgatgg gtaaagcgca aggatcgtcc tggatcttta ttagatcgat taagccaatt
     4201 tttgtctatg gtcattaaat tttccaatat gcggcgtaaa tcgtgcccgc ctcgcggcag
     4261 gatcgtttac acttagcgag ttctggaaag tcctgtggat aaatcgggaa aatctgtgag
     4321 aaacagaaga tctcttgcgc agtttaggct atgatccgcg gtcccgatcg ttttgcagga
     4381 tcttgatcgg gcatataacc gcagacagcg gttcgtgcgt caccctcaag cagggtcttt
     4441 tcgacgtacg tcaacaatca tgaatgtttc agccttagtc attatcgact tttgttcgag
     4501 tggagtccgc cgtgtcactt tcgctttggc agcagtgtct tgcccgattg caggatgagt
     4561 taccagccac agaattcagt atgtggatac gcccattgca ggcggaactg agcgataaca
     4621 cgctggccct gtacgcgcca aaccgttttg tcctcgattg ggtacgggac aagtacctta
     4681 ataatatcaa tggactgcta accagtttct gcggagcgga tgccccacag ctgcgttttg
     4741 aagtcggcac caaaccggtg acgcaaacgc cacaagcggc agtgacgagc aacgtcgcgg
     4801 cccctgcaca ggtggcgcaa acgcagccgc aacgtgctgc gccttctacg cgctcaggtt
     4861 gggataacgt cccggccccg gcagaaccga cctatcgttc taacgtaaac gtcaaacaca
     4921 cgtttgataa cttcgttgaa ggtaaatcta accaactggc gcgcgcggcg gctcgccagg
     4981 tggcggataa ccctggcggt gcctataacc cgttgttcct ttatggcggc acgggtctgg
     5041 gtaaaactca cctgctgcat gcggtgggta acggcattat ggcgcgcaag ccgaatgcca
     5101 aagtggttta tatgcactcc gagcgctttg ttcaggacat ggttaaagcc ctgcaaaaca
     5161 acgcgatcga agagtttaaa cgctactacc gttccgtaga tgcactgctg atcgacgata
     5221 ttcagttttt tgctaataaa gaacgatctc aggaagagtt tttccatacc ttcaacgccc
     5281 tgctggaagg taatcaacag atcattctca cctcggatcg ctatccgaaa gagatcaacg
     5341 gcgttgagga tcgtttgaaa tcccgcttcg gttggggact gactgtggcg atcgaaccgc
     5401 cagagctgga aacccgtgtg gcgatcctga tgaaaaaggc cgacgaaaac gacattcgtt
     5461 tgccgggcga agtggcgttc tttatcgcca agcgtctacg atctaacgta cgtgagctgg
     5521 aaggggcgct gaaccgcgtc attgccaatg ccaactttac cggacgggcg atcaccatcg
     5581 acttcgtgcg tgaggcgctg cgcgacttgc tggcattgca ggaaaaactg gtcaccatcg
     5641 acaatattca gaagacggtg gcggagtact acaagatcaa agtcgcggat ctcctttcca
     5701 agcgtcgatc ccgctcggtg gcgcgtccgc gccagatggc gatggcgctg gcgaaagagc
     5761 tgactaacca cagtctgccg gagattggcg atgcgtttgg tggtcgtgac cacacgacgg
     5821 tgcttcatgc ctgccgtaag atcgagcagt tgcgtgaaga gagccacgat atcaaagaag
     5881 atttttcaaa tttaatcaga acattgtcat cgtaaaccta tgaaatttac cgtagaacgt
     5941 gagcatttat taaaaccgct acaacaggtg agcggtccgt taggtggtcg tcctacgcta
     6001 ccgattctcg gtaatctgct gttacaggtt gctgacggta cgttgtcgct gaccggtact
     6061 gatctcgaga tggaaatggt ggcacgtgtt gcgctggttc agccacacga gccaggagcg
     6121 acgaccgttc cggcgcgcaa attctttgat atctgccgtg gtctgcctga aggcgcggaa
     6181 attgccgtgc agctggaagg tgaacggatg ctggtacgct ccgggcgtag ccgtttttcg
     6241 ctgtctaccc tgccagcggc ggatttcccg aacctcgatg actggcagag tgaagtcgaa
     6301 tttaccctgc cgcaggcgac gatgaagcgt ctgattgaag cgacccagtt ttcgatggcg
     6361 catcaggatg ttcgctatta cttaaacggt atgctgtttg aaaccgaagg cgaagaactg
     6421 cgcaccgtag cgaccgatgg tcaccgtctg gcagtctgtt caatgccaat tggtcaatct
     6481 ttgccaagcc attcggtgat cgtgccgcgt aaaggcgtga ttgaactgat gcgtatgctc
     6541 gacggcggcg acaatccgct gcgcgtgcag attggcagca ataacattcg tgcccacgtt
     6601 ggcgacttta tcttcacctc caaactggtg gatggtcgct tcccggatta ccgccgcgtt
     6661 ctaccgaaga acccggacaa acatctggaa gctggctgcg atctgctcaa gcaggcgttt
     6721 gcccgtgcgg caattctctc taacgagaaa ttccgcggcg tacgtcttta tgtcagcgaa
     6781 aaccagctga aaatcaccgc caacaacccg gaacaggaag aagcggaaga gatcctcgac
     6841 gttacctata gcggtgcgga gatggaaatc ggcttcaacg tcagctatgt gctggatgtt
     6901 ctgaacgcgc tgaaatgcga aaacgtccgc atgatgctga ccgattcggt ttccagcgtg
     6961 cagattgaag atgcggccag ccagagcgcg gcttatgttg tcatgccaat gagactgtaa
     7021 tgtccctcac ccgcttgttg atccgcgatt tccgcaatat cgaaaccgcg gatctcgcct
     7081 tatctcccgg ctttaacttt ctggtaggtg ccaacggcag tggcaaaacc agcgtgctgg
     7141 aagccatcta tacgctcggc catggtcggg cgtttcgtag tttgcagatt ggtcgcgtca
     7201 ttcgccatga gcaggaggcg tttgttctcc acgggcgatt acagggcgaa gagcgcgaga
     7261 cggcgattgg cttaaccaaa gacaaacagg gcgacagcaa agtccgtatt gacggtactg
     7321 acgggcataa ggttgcggag ttggcgcacc tgatgccgat gcagctgata acgcctgaag
     7381 ggtttacttt actcaacggc ggccccaaat acagaagagc attcctcgac tggggatgct
     7441 ttcacaacga acccggattt ttcaccgcct ggagcaatct caagcgattg ctcaagcagc
     7501 gcaatgcggc gctgcgccag gtgacacgtt acgaacagct acgcccgtgg gataaagaac
     7561 tgatcccgct ggcggagcaa atcagcacct ggcgcgcgga gtatagcgcc ggtatcgcgg
     7621 ctgatatggc cgatacttgt aagcaatttc tccctgagtt ctctctgact ttctctttcc
     7681 agcgcggctg ggagaaagag acagaatatg ctgaggtgct ggaacgtaat tttgaacgcg
     7741 atcgccagct aacctacacc gcgcatggcc cgcataaagc ggacttacgc attcgcgccg
     7801 acggtgcgcc ggtggaagat accttatcgc gtgggcagct taagctgttg atgtgcgcct
     7861 tacgtctggc gcaaggagag ttcctcaccc gtgaaagcgg gcggcggtgt ctctacctga
     7921 tagatgattt tgcctctgag cttgatgatg agcgtcgcgg gctgcttgcc agccgcttaa
     7981 aagcgacgca atcacaggtc tttgtcagcg cgatcagtgc tgaacacgtt atagacatgt
     8041 cggacgaaaa ttcgaagatg tttaccgtgg aaaagggtaa aataacggat taacccaagt
     8101 ataaatgagc gagaaacgtt gatgtcgaat tcttatgact cctccagtat caaagtcctg
     8161 aaagggctgg atgcggtgcg taagcgcccg ggtatgtata tcggcgacac ggatgacggc
     8221 accggtctgc accacatggt attcgaggtg gtagataacg ctatcgacga agcgctcgcg
     8281 ggtcactgta aagaaattat cgtcaccatt cacgccgata actctgtctc tgtacaggat
     8341 gacgggcgcg gcattccgac cggtattcac ccggaagagg gcgtatcggc ggcggaagtg
     8401 atcatgaccg ttctgcacgc aggcggtaaa tttgacgata actcctataa agtgtccggc
     8461 ggtctgcacg gcgttggtgt ttcggtagta aacgccctgt cgcaaaaact ggagctggtt
     8521 atccagcgcg agggtaaaat tcaccgtcag atctacgaac acggtgtacc gcaggcaccg
     8581 ttggcggtta ccggtgagac tgaaaaaacc ggcaccatgg tgcgtttctg gcccagcctc
     8641 gaaaccttca ccaatgtgac cgagttcgaa tatgaaattc tggcgaaacg tctgcgtgag
     8701 ttgtcgttcc tcaactccgg cgtttccatt cgtctgcgcg acaagcgcga cggcaaagaa
     8761 gaccacttcc actatgaagg cggcatcaag gcgttcgttg aatatctgaa caagaacaaa
     8821 acgccgatcc acccgaatat cttctacttc tccactgaaa aagacggtat tggcgtcgaa
     8881 gtggcgttgc agtggaacga tggcttccag gaaaacatct actgctttac caacaacatt
     8941 ccgcagcgtg acggcggtac tcacctggca ggcttccgtg cggcgatgac ccgtaccctg
     9001 aacgcctaca tggacaaaga aggctacagc aaaaaagcca aagttagcgc caccggtgac
     9061 gatgcgcgtg aaggcctgat tgcggtcgtt tccgtgaaag tgccggaccc gaaattctcc
     9121 tcccagacca aagacaaact ggtttcttct gaggtgaaat cagcggttga acagcagatg
     9181 aacgaactgc tggcagaata cctgctggaa aacccaaccg acgcgaaaat cgtggttggc
     9241 aaaattatcg atgctgcccg tgcccgtgaa gcggcgcgtc gcgcgcgtga aatgacccgc
     9301 cgtaaaggtg cgctcgactt agcgggcctg ccgggcaaac tggcagactg ccaggaacgc
     9361 gatccggcgc tttccgaact gtacctggtg gaaggggact ccgcgggcgg ctctgcgaag
     9421 caggggcgta accgcaagaa ccaggcgatt ctgccgctga agggtaaaat cctcaacgtt
     9481 gagaaagcgc gcttcgataa gatgctctct tctcaggaag tggcgacgct tatcaccgcg
     9541 cttggctgtg gtatcggtcg tgacgagtac aacccggaca aactgcgtta tcacagcatc
     9601 atcatcatga ccgatgcgga cgtcgacggc tcgcacattc gtacgctgct gttgaccttc
     9661 ttctatcgtc agatgccgga aatcgttgaa cgcggtcacg tctacatcgc tcagccgccg
     9721 ctgtacaaag tgaagaaagg caagcaggaa cagtacatta aagacgacga agcgatggat
     9781 cagtaccaga tctctatcgc gctggatggc gcaacgctgc acaccaacgc cagcgcaccg
     9841 gcattggctg gcgaagcgtt agagaaattg gtgtctgagt acaacgcgac gcagaaaatg
     9901 atcaaccgca tggagcgtcg ttatccgaaa gcaatgctga aagagcttat ctatcagccg
     9961 acgctgacgg aagccgacct ctctgatgag cagaccgtta cccgctgggt gaacgcgctg
    10021 gtcagcgaac tgaacgacaa agaacagcac ggcagccagt ggaagtttga tgtccacacc
    10081 aatgccgaac aaaacctgtt cgagccgatt gttcgcgtgc gtacccacgg tgtggatact
    10141 gactatccgc tggatcacga atttatcact ggtggcgaat atcgtcgtat ctgcacgctg
    10201 ggtgagaaac tgcgtggctt gctggaagaa gatgcattta tcgaacgtgg cgagcgtcgt
    10261 cagccggtag ccagcttcga gcaggcgctg gactggctgg tgaaagagtc tcgtcgcggt
    10321 ctctccatcc agcgttataa aggtctgggc gagatgaacc cggaacagct gtgggaaacc
    10381 accatggacc cggaaagtcg tcgtatgctg cgcgttaccg ttaaggatgc gattgccgct
    10441 gaccagttgt tcaccacgct gatgggcgac gccgtagaac cgcgtcgtgc gtttattgaa
    10501 gagaacgccc tgaaagcggc gaatattgat atttaatggc gttaaccatg cgagcgtgcc
    10561 tgatgcgcta cgcttattag tcctacgaga ctcctgcaat atattgattt tgcatgattt
    10621 tgtaggccgg ataaggcgtt cacgccgcat ccggcaagaa gctataagaa aagggcggag
    10681 atcatctccg ccctttttat ttctgcaatc cgcaattatc atcccgcaaa tctatacttt
    10741 ttgggtcaac acgtaacgct ttacaggtga ttatcatggg gctttttgat gaagttgtcg
    10801 gtgcctttct gaaaggcgat gcggggaaat atcaggctat tttaagttgg gttgaggagc
    10861 agggcggcat tcaggtgctg ctggaaaaac tgcaaagtgg cggcttaggg gccattctct
    10921 caacctggct gagtaatcaa cagggcaatc aatcggttag tggcgagcaa ctggaatcgg
    10981 cgctcggcac aaatgcggtg tccgatctcg ggcaaaaact tggcgtggat accagtacag
    11041 cttccagttt actggcagag caattgccga agattattga tgcgctctca ccgcaaggtg
    11101 aagtgtcacc acaagccaat aacgatctgc tttccgcagg tatggaactg ctgaaaggga
    11161 aactcttccg ctaagcaaat ggggagcatg caacccgcgt tgcatccccc ttttccctcg
    11221 ggtgttgtac tgatttttga gcggaatcgc gttagcatgg gtcaggaacc aatctacctg
    11281 gggaactcat ggctatcaaa ctcattgcta tcgatatgga tggcaccctt ctgctgcccg
    11341 atcacaccat ttcacccgcc gttaaaaatg cgattgccgc agctcgcgcc cgtggcgtga
    11401 atgtcgtgct aacgacgggt cgcccgtatg caggtgtgca caactacctg aaagagctgc
    11461 atatggaaca gccgggcgac tactgcatta cttataacgg cgcgctggta cagaaggccg
    11521 ctgatggtag caccgtggcg caaactgctc tcagctatga cgactatcgt ttcctggaaa
    11581 aactctctcg cgaagtcggt tctcatttcc acgccctgga ccgcaccacg ctgtacaccg
    11641 ccaaccgtga tatcagctac tacacggtgc atgaatcctt cgttgccacc attccgctgg
    11701 tgttctgcga agcggagaaa atggacccca atacccagtt cctgaaagtg atgatgattg
    11761 atgaacccgc catcctcgac caggctatcg cgcgtattcc gcaggaagtg aaagagaaat
    11821 ataccgtgct gaaaagtgcg ccgtacttcc tcgaaatcct cgataaacgc gttaacaaag
    11881 gtacgggggt gaaatcactg gccgacgtgt taggtattaa accggaagaa atcatggcga
    11941 ttggcgatca ggaaaacgat atcgcaatga ttgaatatgc aggcgtcggt gtggcgatgg
    12001 ataacgctat tccttcggtg aaagaagtgg cgaactttgt caccaaatct aaccttgaag
    12061 atggcgtggc gtttgctatt gagaagtatg tgctgaatta atctgtgggc gggcaaacag
    12121 catgaatgcg tttgcccgct tactgattat tggtttactg catatacaac cagaaattgc
    12181 agcggtgcag taatgatgtc cagcgtaaca gtcactggca gtaatgctgc ggaaacacca
    12241 tccggaatgc cactgggtga atagtattca taaaatcgca ctttaaatgg atggtagaac
    12301 gccatcacct ttgagtaatc catgttttta tttttctcat ggattgtgcc ttttaggttt
    12361 tccagtgacc tggtacagtg ttgaacacca caggcaaaac cataatgcgt tgccagtgcc
    12421 ttttcttctt cgttattcca gtagtagtaa agcgatattg tgccggtaaa tttctttttc
    12481 cccgcattta aaacaaatct tgcgtcatcg gcaacctgaa tattgtgccg gttaagtgct
    12541 ggatcattga gcatcttaac gatgtcatcg gcaccatcag tgataaggta atccagcgat
    12601 tcccctacaa aaacatagcc ttttgttcca ttactatctt ttgcctgcga tagcccagtg
    12661 atggtgtctg actgcgattg atactttcct gaactaccct ttgcggtatg ggaaaccaga
    12721 gcatacgaga tacagcctga aagcatcagc gccagtggga ataaaccggc agccttaaaa
    12781 aatcccttaa aattcaactt catgattaat cctttaaacg agtagctaag ccgttgaaag
    12841 catactcttc tttccctcct gtttagcgcc tgatatccct tttcagattt ctgcccgacg
    12901 catgtcattt ttttatgcat tgttcttttt gtgatctaaa ttgtagtgca acaatataag
    12961 tttgtactac attacgcaca cggcaaacgc gaacgtcatc acgctggtac tacaaagttg
    13021 ccgcgttatg catcgatcgg ggtaaagtag agaagaacat acagagcaca aggactctcc
    13081 atgactctca ataaaaccga tcgcattgtc attacgctgg gtaaacagat cgttcacggc
    13141 aaatacgtac ctggctcgcc acttccggct gaggcggagc tctgtgaaga gtttgcaacc
    13201 tcgcgcaaca tcatccgtga ggtgttccgt tcgctgatgg cgaagcggct gattgaaatg
    13261 aaacgttatc gcggggcgtt tgtggcaccg cgtaaccagt ggaattacct cgacactgac
    13321 gtactgcaat gggtgttgga aaacgactac gacccacggc ttatcagtgc catgagcgaa
    13381 gtgcgaaatc tggtggaacc ggcgattgcc cgttgggcag cagagcgcgc gacttccagc
    13441 gatctggcgc agattgaatc ggcgctgaac gagatgattg ccaacaatca ggaccgcgaa
    13501 gcgtttaacg aagcggatat tcgctaccac gaggcggtgc tgcagtcggt gcataacccg
    13561 gtgttacagc aacttagcat tgcgatcagt tcgctgcagc gggcggtttt tgaacgaacc
    13621 tggatgggcg atgaggccaa catgccgcaa acgctccagg aacataaggc gctgttcgat
    13681 gcgatacggc atcaggacgg cgatgcggca gagcaggcgg cgcttaccat gatcgccagc
    13741 tcgacacgaa ggttaaagga aatcacatga cagctcgcta catcgcaatt gactggggat
    13801 cgaccaatct gcgcgcctgg ctttatcagg gcgaccactg cctggagagc aggcaatcag
    13861 aagcaggcgt cacgcgcctg aacggaaaat ctccggctgc ggtgttagca gaagtcacga
    13921 ccgactggcg tgaagagaat acgccagtgg tgatggctgg aatggtcggc agcaatgtcg
    13981 gctggaaagt tgcaccgtat ttatccgttc ctgcccgttt ttcgtctatt ggcgaacaat
    14041 taacgtctgt tggcgacaat atctggatta ttcccggatt atgcgtctct catgatgata
    14101 accacaatgt gatgcgcggc gaagaaacac aattaatcgg cgcgcgagct ctggctcctt
    14161 cctctcttta tgttatgccc ggtacgcatt gcaaatgggt gcaggccgat agccagcaaa
    14221 tcaacgattt tcgcaccgtg atgaccggtg aattacatca tttactgtta aatcactcat
    14281 tgattggcgc aggtttgccg ccgcaggaaa actctgccga tgccttcgcg gctggccttg
    14341 agcgcggcct taatgcgccc gccatattgc cgcagctttt tgaagttcgc gcctcgcatg
    14401 tgctgggaac acttccccgc gaacaggtca gcgaatttct ctctggtttg ttgattggcg
    14461 cagaggttgc cagtatgcgc gactatgtga cccatcaaca cgccatcacc cttgtcgccg
    14521 gaacatcgct gaccgcgcgc taccagcaag cctttcaggc gatgggttgc gacgtgacgg
    14581 cggtggcggg cgacacggca tttcaggctg gtataaggag catcgctcat gcagtggcaa
    14641 actaaacttc cgctgatcgc cattttgcgc ggcattaagc ccgacgaggc gctggcgcat
    14701 gttggcgcgg tgattgacgc cgggttcgac gcggttgaaa tcccgctgaa ttccccacaa
    14761 tgggagcaaa gcattcccgc catcgttgat gcgtatggcg acaaggcgtt gattggcgca
    14821 ggtacggtac tgaaacctga acaggtcgat gcgctcgcca ggatgggctg tcagctcatc
    14881 gttacgccca atatccatag tgaagtgatc cgccgtgcgg tgggctacgg catgaccgtc
    14941 tgccccggct gcgcgacggc gaccgaagcc tttaccgcgc tcgaagcggg cgcgcaggcg
    15001 ctgaaaatat ttccgtcatc ggcttttggt ccgcaataca tcaaagcgtt aaaagcggta
    15061 ttgccatcgg acatcgcagt ctttgccgtt ggcggcgtga cgccagaaaa cctggcgcag
    15121 tggatagacg caggttgtgc tggggcgggc ttaggcagcg atctctatcg cgccgggcag
    15181 tccgtagaac gcaccgcgca gcaggcagca gcatttgtta aggcgtatcg agaggcagtg
    15241 caatgaaaat caccaaaatt accacgtatc gtttacctcc ccgctggatg ttcctgaaaa
    15301 ttgaaaccga tgaaggcgtg gtcggttggg gcgagcccgt gattgaaggc cgcgcccgta
    15361 cggtggaagc ggcagttcac gagctgggtg actatttgat tggtcaggat ccttcgcgca
    15421 tcaatgactt atggcaagtg atgtatcgcg ccggatttta tcgtggcggt ccaatcctga
    15481 tgagcgccat tgccgggatc gaccaggcgt tatgggatat caaaggcaaa gtgctgaatg
    15541 cgccggtctg gcaactgatg ggcggcctgg ttcgcgacaa aattaaagcc tacagttggg
    15601 ttggcggcga tcgtccggcg gatgttatcg acggcattaa aacgctacgc gaaatcggct
    15661 tcgatacctt caaactgaac ggttgtgaag aactggggct aattgataac tcccgcgcgg
    15721 tagatgcggc agtcaacacc gtggcacaaa ttcgtgaagc ttttggcaat cagattgagt
    15781 ttggtcttga tttccacggt cgcgtcagcg cgccaatggc gaaagtgctg attaaagaac
    15841 tggagccgta tcgcccgctg tttattgaag agccggtgct ggcggaacaa gccgaatact
    15901 acccgaaatt ggcggcacaa acgcatattc cactggcggc gggtgagcgc atgttctcac
    15961 gtttcgattt taaacgcgtg ctggaggcag gcggtatttc gattctgcaa ccggatctct
    16021 cccatgcagg cggtattacc gaatgctaca aaattgctgg aatggcagaa gcctatgatg
    16081 tgacccttgc gccgcactgt ccgctcggac cgattgcact ggcggcttgc ctgcatatcg
    16141 actttgtttc ctataacgcg gtacttcagg aacaaagtat gggcattcat tacaacaaag
    16201 gcgcggagtt actcgacttt gtgaaaaaca aagaagactt cagtatggtt ggcggcttct
    16261 ttaaaccgtt aacgaaaccg ggcttaggtg tggaaatcga cgaagctaaa gttattgagt
    16321 tcagtaaaaa tgccccggac tggcgtaatc cgctctggcg tcatgaagat aacagcgtag
    16381 cagagtggta attcctgcca cgtaagcccc tcatcgggca ctaaaacagc aatacaaaaa
    16441 tataaccctc tgtaaattac agggcatggt gagcggcttc gctatgccca aaatctggag
    16501 aaagctcgct atggatattc ccgttaatgc agcaaagccg gggcgtcggc gttatctgac
    16561 gctggtgatg atctttatta cggtggtcat ttgttatgtt gaccgcgcta acctggccgt
    16621 ggcttccgcc catattcagg aagagttcgg cattacgaaa gcggaaatgg gctatgtatt
    16681 ttcggccttc gcctggcttt atacgctatg ccagatcccc ggcggttggt ttttagatcg
    16741 cgtaggttct cgcgtgactt attttattgc gatatttggt tggtcagtgg cgactttatt
    16801 ccagggcttt gccacgggat taatgtcatt aattggtctg cgtgcgataa ccggtatttt
    16861 cgaagcgccc gctttcccga ccaataaccg gatggtgacc agctggttcc cggaacatga
    16921 acgcgcttcc gccgttggtt tttatacgtc tggtcagttt gtcggtctgg cgtttttgac
    16981 tccgctgctg atctggattc aggagatgtt gagctggcac tgggtgttca ttgtcactgg
    17041 tggtatcggc attatctggt cgctgatttg gtttaaggtt tatcagccgc cgcgcctgac
    17101 caaaggtatc agcaaagctg aactggatta cattcgtgat ggcggcggtc tggtggatgg
    17161 tgatgcgccg gtgaagaaag aggcgcgtca gccgttaaca gccaaagact ggaaactggt
    17221 gttccatcgt aaactgatcg gcgtttatct tgggcaattt gcggtggctt ctacactgtg
    17281 gtttttctta acctggttcc cgaactattt aacccaggaa aaaggaatca cggcgctgaa
    17341 agcaggcttt atgaccacgg tgccatttct cgcggcgttt gtcggcgtcc tgctctctgg
    17401 ctgggtagcg gatctgctgg tacgtaaggg cttttcactg ggctttgcgc gtaaaacgcc
    17461 gattatctgc ggcttgctga tctccacctg cattatgggc gctaactaca ctaacgatcc
    17521 gatgatgatt atgtgcctga tggcgctggc attcttcggt aacggttttg cttcgattac
    17581 ctggtcgctg gtttcttctc tggcaccgat gcgcctgatt ggtttaaccg gcggcgtgtt
    17641 taacttcgcc ggtggtctgg gcggcatcac cgttccgctg gtggtggggt acctggcgca
    17701 gggttacggt ttcgcacctg cactggttta tatctccgcc gtcgcgttga ttggcgcgct
    17761 ctcttatatc ctgctggtgg gcgatgtgaa gcgcgttggc taatccttca actgtggaat
    17821 atgtgccaca ccactgcgca taatccactt tcttaaagcc ggtgcggtca ggcatcggct
    17881 ttttactatc ttgccgaaga gttttaaacg cagtttgcgg gtggcgcgcc agtaagcggt
    17941 attgagcttc tctggctgct tcagtaacac cgaacgcaga atgtctgcgc tatccagcgc
    18001 atagctaatc ccctccagcg agctggcgct gataaatccc gccgcttcac caatcagaaa
    18061 ggcgttgtcc ttaccgcaga caaaatcctg ccagcgtgag ggaaacagca ccgtgcattt
    18121 ttcgcttttc accgccttac caaactggaa ctgaaaggcg ctcattttct ctttcagcgt
    18181 cgtgaaacgc gtctgaccgt ctttcattgg ataagcaccg ccaaagataa aataaccgtc
    18241 tttgctgata ctccatgaat aacagttagt tatcgaatta tcaaagatgc aggagtagaa
    18301 cggcaccgga tgtttctccg cgaaccactg ctggatagcg acatatttac ggatttgatg
    18361 atccgggtag agatgtcgcc gcaccatcga gtttgcgcca tcggcaccga ccagatagcg
    18421 ggcggtaata tgttgctccc agccgtctgc acgaaaaatg acatgccatt tatcatcctc
    18481 acgccagatt tttcggcaca ggctatcgtg ataaacctca acgctggtgg ggatcagcga
    18541 tttcatccac aagtcgaaag catggcgatt aatattgata tagcttcgct ggtagttacg
    18601 cgtgagcgat gcggcgacgt cgacagtttt gacgctgaaa atctgtggat tggcgatcac
    18661 atcgacagga agcgtcagtc catcgcgaat aaaagatcgc tgcgcgtccg gtgccagcag
    18721 accgccgcaa ggtttgctga aaccttcagt accacacagg tgctttttat ccagcgcgat
    18781 cacctgcatt ttgcctgcta attttcgcgc caacgccgat cctgccgggc cgaggccaat
    18841 aatcgccacg tcgaaatgtt ccatcttttc actcacattc atcacgatgg caaaagacta
    18901 ttgtctgaaa gtgaagtcct tgtgaagtat gatggtggga cccgttttgt atcaggaccg
    18961 cgccatgaaa cagataacct ttgctccccg taatcacctg ctcaccaata ccaatacctg
    19021 gacgcccgac agccagtggc tggtatttga cgtgcgtcct tctggggcgt cgtttaccgg
    19081 cgagaccatt gagcgtgtga atatccatac cggcgaggtc gaggttatct atcgcgcgtc
    19141 acagggcgca cacgtcggcg tggtgaccgt tcatccgaag tcagagaaat atgtctttat
    19201 tcacggccct gaaaatcctg atgaaacatg gcattacgac ttccatcatc gacgcggagt
    19261 cattgctgaa ggcggcaagg tgagcaatct cgatgcaatg gatattaccg cgccgtatac
    19321 gccaggagcg ctgcgcggcg gcagccatgt gcatgtcttt agcccgaacg gtgaaagggt
    19381 gagctttacc tataacgacc atttaatgca tgaactcgat ccggcgctgg atttgcgaaa
    19441 cgtcggtgtt gctgcgccgt ttggcccggt caacgtacaa aagcagcatc cgcgtgaata
    19501 cagcggtagc cactggtgcg tgctggtgag taaaaccacg cccacgccgc agcctggcag
    19561 cgatgaaatc aatcgtgctt atgaagaagg atgggtagga aatcacgcgc tggcgtttat
    19621 tggcgacaca ctttcgccaa agggcgagaa agtgccggag ctgtttatcg ttgagttacc
    19681 gcaagatgaa gctggctgga aagcggcagg tgatgcgccg ttaagtggaa cggaaaccac
    19741 cctgcccgcg ccaccgcgcg gcgtcgtgca gcgacgttta acctttaccc accatcgggc
    19801 ttatccgggg ttagtcaacg tcccgcgcca ctgggtgcgc tgtaatccgc agggtacgca
    19861 aatcgcgttt ttaatgcgtg atgataacgg cattgtgcaa ctgtggctta tctcgccaca
    19921 gggcggcgag ccgcgccagt taacccataa caaaacggat attcagtctg catttaactg
    19981 gcatccgtca ggagaatggt tgggctttgt gctggataat cgaattgctt gtgcccatgc
    20041 gcaaagcggc gaggttgagt atttaaccga aaaccacgcc aatccacctt ctgcggacgc
    20101 cgtggtcttc tcgccggatg gtcaatggct ggcgtggatg gaaggtggcc agctgtggat
    20161 caccgaaact gatcgctaaa tcagttatca ggcattctgg ccggtggaat aaccgcattg
    20221 gtggcttgcg cgttggcttc gcttttttca acgcgtgagc gcaccgagct gtctttgcgg
    20281 aacacatccc acggcagtaa aagcgtgtcc atcacagcgg taaacggcat atcgagaatc
    20341 gccagcgact tagtgcccca gttggtttca tcgtcgccaa tcatggttgc gctggcgcgg
    20401 gtgccgggat acgttccttc tttccctccg gtatgggaca tcacgctgga gcatccaccg
    20461 agtaatgtca ttccgctgca tatcatgaac gctaacaaca catttcttat cataataaat
    20521 tcatctgttg atcgtgggtg ttggcctgat gagttatagc gatcccttgc tgaaaataac
    20581 atcatcatta cgtcgcactg tggcggctat cgcactttaa cgtttcgtgc tgccccctca
    20641 gtctatgcaa tagaccataa actgcaaaaa aaagtccgct gataaggctt gaaaagttca
    20701 tttccagacc catttttaca tcgtagccga tgaggacgcg cctgatgggt gttctggcta
    20761 cctgacctgt ccattgtgga aggtcttaca ttctcgctga tttcaggagc tattgattat
    20821 gcgtaacttt gatttatccc cgctttaccg ttctgctatt ggatttgacc gtttgtttaa
    20881 ccacttagaa aacaaccaga gccagagtaa tggcggctac cctccgtata acgttgaact
    20941 ggtagacgaa aaccattacc gcattgctat cgctgtggct ggttttgctg agagcgaact
    21001 ggaaattacc gcccaggata atctgctggt ggtgaaaggt gctcacgccg acgaacaaaa
    21061 agagcgcacc tatctgtacc agggcatcgc tgaacgcaac tttgaacgca aattccagtt
    21121 agctgagaac attcatgttc gtggcgctaa cctggtaaat ggtttgctgt atatcgatct
    21181 cgaacgcgtg attccggaag cgaaaaaacc gcgccgtatc gaaatcaact aattccctaa
    21241 ggccgcctgg cgcggcctga catctccatg ctcgccgtca gggagcatat gcgaatcttc
    21301 ggatttgcag gtacttactc gcttcttaga aggagaaatg actatgcgta acttcgattt
    21361 atccccactg atgcgtcaat ggatcggttt tgacaaactg gccaacgcac tgcaaaacgc
    21421 cggtgaaagc cagagcttcc cgccgtacaa cattgagaaa agcgacgata accactaccg
    21481 cattaccctt gcgctggcag gtttccgtca ggaagattta gagattcaac tggaaggtac
    21541 gcgcctgagc gtaaaaggca cgccggagca gccaaaagaa gagaaaaaat ggctgcatca
    21601 agggcttatg aatcagccat ttagcctgag ctttacgctg gctgaaaata tggaagtctc
    21661 tggcgcaacc ttcgtaaacg gtttactgca tattgattta attcgtaatg agcctgaacc
    21721 catcgcagcg cagcgtatcg ctatcagcga acgtcccgcg ttaaatagct aactagctat
    21781 tctctttgcc ccgccattct ggtggggctt tttttgtctt acttactcaa caatgcgtta
    21841 aatttcgact gtttaagata tttcggcacg ttctttcccg tgataatgtg cgctataagg
    21901 caaatctgct tcaccgattc agccataatc cgtagcaatt aaaatgttat ctcaagagaa
    21961 atggacgatg agtgatatag cattaacggt cagtattctg gctttggtgg cagtcgtcgg
    22021 tttgtttatc ggcaacgtca aatttcgcgg cataggatta ggtattggcg gcgtgctgtt
    22081 tggtgggatc atcgtcggcc attttgtttc tcaagcgggg atgacattaa gtagcgatat
    22141 gctgcatgtt attcaggaat ttggcctgat cctgttcgtt tataccatcg ggattcaggt
    22201 agggccgggc ttctttgcct cattgcgtgt ctccggatta cgcctcaacc tgtttgctgt
    22261 tctgatcgtc atcatcggtg gtctggttac cgccatcctg cataaactgt ttgatattcc
    22321 actgccggta gtgctgggga ttttctccgg tgcggttacc aatacgccag cgctgggggc
    22381 agggcagcag attttgcgcg acctgggtac accaatggaa atggtcgatc agatggggat
    22441 gagttacgcg atggcgtatc cattcggcat ttgcgggatt ttgttcacca tgtggatgtt
    22501 gcgggttatt ttccgcgtca atgtcgagac agaagctcag cagcacgagt cttcacgcac
    22561 caatggcggc gcgctgatca agactatcaa tattcgcgtt gagaacccta acctgcatga
    22621 tttagccatt aaagatgtac cgattctcaa cggcgacaaa attatctgct cgcgtctgaa
    22681 acgcgaagaa accctaaaag ttccttcgcc agataccatt atccaactgg gcgatttgct
    22741 gcatctggtg gtgcagccag cggatttaca taatgcgcaa ctggtgattg gtcaggaggt
    22801 cgatacttcg ctgtccacga aaggcactga tttgcgcgtc gagcgtgtgg tggtcaccaa
    22861 tgaaaacgtg ctcggaaaac gtattcgcga cctgcacttt aaagaacgct atgacgttgt
    22921 tatctcgcgc ctgaaccgtg ccggggtcga actggtcgcc agtggcgata tcagcctgca
    22981 gttcggcgat atcctcaacc tggtggggcg tccgtccgca attgatgccg ttgccaatgt
    23041 gctggggaat gcgcagcaaa aactgcaaca ggttcagatg ctgccagtgt ttattggcat
    23101 cgggcttggc gtattgttag gttctattcc cgtctttgtg ccaggattcc cggccgcgtt
    23161 gaaactgggg ctggcgggcg gtccgctgat tatggcgttg atcctcgggc gtatcggcag
    23221 tatcggcaag ctgtactggt ttatgccgcc aagtgccaac ctcgcgctgc gggagctggg
    23281 gatcgtgctg ttcctctcgg tcgttggtct gaaatctggt ggggattttg taaataccct
    23341 ggtcaatggc gaagggctaa gctggattgg ttatggagcc ctgatcaccg ccgttccgct
    23401 gattactgtt ggcattctgg cgcggatgtt agccaaaatg aattacctga ccatgtgcgg
    23461 gatgctggca ggttccatga ccgatcctcc ggcgctggcg tttgctaata atcttcatcc
    23521 aaccagcggt gcggcggcgc tctcttacgc cactgtctat ccgttagtga tgttcctgcg
    23581 cattatcacc ccccaattac tggcggtgct cttctggagt atcggttaac tcttttccgg
    23641 gtggagtcgc cgtaaatggt actccacctg gtattcgctg gcatttctga acatcactga
    23701 ataattcaaa aactccccgc tctcactata tgacaacgag gtgatccgca gcagtggcgt
    23761 gtgttccgct accttcattt gccgcgccag cagtctatcg gcgagcaccg gcgtcaggct
    23821 ttcataatta ccgccaatca aaatcccaca ctcttgttca atatattcaa acttcgaccc
    23881 ttccagatgt tgcagcgaaa ggttacggaa cagttttacc ggcatatagc tgtcttcctg
    23941 catcagcggt ttcccttcca caaaacgaac gcgacgggag aagtagatct gctcgttgat
    24001 ttgaatccgt aactggctgg caatggccgg aggcgcaggc atgatttcaa aaatcagcac
    24061 ctggctggtg accgtttttc cctgccgttt taacacctcc accagcccgg tcaggctggc
    24121 ggtttgatgc agtacatctt tgcgcaccag gtaagtgccg ctgccgtggc ggcggaccac
    24181 caatccccac gctaccagca ggtcaatggc tttacggatg gtcatccgcg ataccgcaaa
    24241 ttcttccgcc aacttttttt caccgggaag aaggctgttt agcgtgaaat ccgcggaatt
    24301 aagtcgaatt cttaaccgct ccgcaatgct tttgtagatc atctctagat ctcttttttg
    24361 cctgcaagcc cgggtaatgc gcccacaagt tctgcttaat cgattgaaaa aacatctttt
    24421 tttaaagatg tgttcgatgc tgtgtaccag tgctcacaga tgtctacttt ttcgcgaaaa
    24481 cgtagatctc taccgcccaa cgaaaagcat gaaagcgatc acgaatccca ttgggtcgtc
    24541 atgttctgcc gatcgcatct tcctatcctc gctccaggcc tgccgcataa ccaatcaggc
    24601 ttcctactta cagaattgag aaaagaggat gtggaaatgc tcagtcaaat tcaacgcttt
    24661 ggcggcgcga tgttcacgcc agtgctgctg tttcccttcg ccgggattgt ggtgggtctt
    24721 gccatcttgc tgcaaaaccc gatgtttgtc ggggaatcac tgaccgatcc gaacagttta
    24781 ttcgcgcaaa tcgtacacat tattgaagag ggcggttgga cggtattccg taatatgccg
    24841 ctgatttttg ctgtcggttt acccattggc cttgctaagc aagcgcaggg gcgtgcttgt
    24901 ctggcggtga tggtgagttt cctgacctgg aactatttca tcaacgcgat gggaatgacc
    24961 tggggaagct acttcggcgt cgatttcact caggacgcgg tggcaggtag cggtctgaca
    25021 atgatggccg ggattaaaac cctcgatacc agcattatcg gcgcaattat catttccggc
    25081 attgtgacgg cgctgcataa ccgtctgttc gataaaaaac tgccggtttt tctcggcatt
    25141 ttccagggga cgtcttatgt ggtgattatc gccttcctgg tgatgatccc ctgtgcctgg
    25201 ctgacgttgc tcggctggcc aaaagtacaa atggggattg aatctctgca agcgttcctg
    25261 cgttcggcgg gtgcacttgg ggtctgggtt tacaccttcc tcgaacgtat tctgatccca
    25321 accggtttac accacttcat ctacggacag tttatctttg gtccggcagc tgttgaaggc
    25381 ggcattcaga tgtactgggc gcagcatctg caagagttca gtttgagcgc cgagccgctg
    25441 aaatcgttgt tcccggaagg cggttttgcc ctgcacggta actcaaaaat ctttggtgcc
    25501 gtgggcattt ctttagcgat gtacttcact gccgcaccgg aaaatcgggt aaaagtggcg
    25561 ggcttgctga ttcccgcaac cttaaccgcc atgctggtgg gaattaccga accgctggaa
    25621 tttaccttcc tgttcatttc accgttgctg tttgcggtac acgccgtgct ggcggcctca
    25681 atgtcgaccg taatgtatct ctttggtgtg gtgggcaaca tgggcggagg tctgattgac
    25741 caggttttac cgcaaaactg gatcccgatg ttcagcaacc acgcggatat gatgctgacc
    25801 caaatcgcca ttgggttgtg ctttaccctg ctgtacttcg tggttttccg cacactgatt
    25861 ctgcagttca acatgtgcac gccgggacgt gaagatgcgg aagtgaaact ctactcaaaa
    25921 gccgaataca aagcctcgcg aggccaaacc accgcggcag agccaaaaaa agagctggat
    25981 caggctgccg gtatcctgca agccctgggc ggggtcggca atatctccag cattaacaat
    26041 tgcgcgacgc gtttacgtat tgcactgcat gacatgtcac aaacgctgga tgacgaagtc
    26101 tttaaaaagc tgggagcgca cggcgtcttc cgtagtggcg atgccattca ggtgatcatt
    26161 ggtctgcatg tatcccagct gcgtgaacag ctcgatagct taattaattc tcatcaatca
    26221 gcagaaaatg ttgccattac ggaggcagta taatgaccaa attctcagtg gttgtcgcag
    26281 gcggtggaag cacctttacg ccaggcatcg tgttgatgct cctggcgaat caggaccgtt
    26341 tcccgcttcg tgcactgaaa ttttatgata acgatggtgc gcggcaggaa gtgattgccg
    26401 aagccagtaa agtcatcctt aaagagaaag cgccggacat tgcgtttagt tacaccaccg
    26461 atcctgaagt ggcattcagc gacgttgatt ttgtcatggc gcacatccgc gtcggcaagt
    26521 acccgatgcg cgaactggat gaaaaaatcc cactgcgcca cggtgttgtt ggtcaggaaa
    26581 cttgcggacc cggcggaata gcgtacggca tgcgttccat tggcggcgtc ctggaactgg
    26641 tggattatat ggaaaaatat tcaccaaatg cctggatgct caactactcc aacccggcaa
    26701 ccattgtcgc agaagccacg cgtcgtctgc gcccgaatgc gaaaatcctc aacatctgtg
    26761 acatgccaat cggtattgaa agccggatgg cgcaaattgt tgggctgcaa gatcgcaaac
    26821 agatgcgcgt gcgctactac ggcctgaacc actttggctg gtggacatca attgaagatt
    26881 tgcagggcaa cgacctgatg ccccagctgc ggcaatatgt ctctaagcac ggttatgttc
    26941 caccgctgca agatacacat actgaagcga gctggaacga cacctatgca aaagcgcggg
    27001 atgtccaggc actggacccg gatacattac caaacaccta tctgaaatat tatctcttcc
    27061 cggattatgt cgttcagcat tccaaccctg aacatacccg cgcgaatgag gtgatggaac
    27121 atcgcgagaa acaggttttc gatgcttgcc gcgccattac ggcggcagga aattcagcgg
    27181 cgggcaagct ggaaattgac gaacatgcgt catacatcgt cgatctggcg gcggcaattg
    27241 ccttcaacac tcaggagcgg atgttgctga ttgtgcctaa caacggggca attcataact
    27301 ttgatgatga agcgatggtc gaaatcccgt gtctggtcgg gcacaacgga ccagaaccac
    27361 tggtggtcgg cgatatcccg cagtttcaga aagggttaat gagtcagcaa gtggcggtgg
    27421 aaaaactggt cgtggacgcc tgggaacagc gttcatatca gcacctgtgg caggcgatta
    27481 cgttatcgaa aacggtaccg agcgcctcgg tcgccaaagc tattctggat gagttgctgg
    27541 aggccaacaa agcgtactgg ccagagttac gttaattgtg tggactggca tccgttgggt
    27601 gctggtccgt attctttagc acggctgacg gcgcataacc aaatttatcc ggtgcgccgt
    27661 aaaaccccgt ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg
    27721 ctgttgttca acgtattggc ggatgacgga tattggcgca cctccgcagc tactggcaaa
    27781 atagccagga ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc
    27841 tcgtcgcagt cacctacccg atacgccttt gaggctgttt accagactgg atatcgccag
    27901 tttgggagga tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag
    27961 ttcagcttca aaatcagcac atacatttga aaagtaagcg cgtagttttt ctgtcgcgtc
    28021 atggtcaaaa atctgacgtc ggtatctggt gacaaagacc aggtgaacat gcatcaggaa
    28081 aacacaatgc cttccccgtc ggatatcggt ttctttattc atagaccaaa gtatcatttg
    28141 gcctgtgaaa cgattacagg catttaaatt ccagttaaga ccaggtggtc aacaggag
//
LOCUS       DAZKIV010000048        28140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZKIV010000048 DAZKIV010000000
VERSION     DAZKIV010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.048"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..877
                     /gene="ygcG"
                     /locus_tag="ACHOT6_002043"
     CDS             5..877
                     /gene="ygcG"
                     /locus_tag="ACHOT6_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9947264.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(937..2235)
                     /gene="eno"
                     /locus_tag="ACHOT6_002044"
     CDS             complement(937..2235)
                     /gene="eno"
                     /locus_tag="ACHOT6_002044"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFP9947265.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2323..3960)
                     /gene="pyrG"
                     /locus_tag="ACHOT6_002045"
     CDS             complement(2323..3960)
                     /gene="pyrG"
                     /locus_tag="ACHOT6_002045"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFP9947266.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4188..4979)
                     /gene="mazG"
                     /locus_tag="ACHOT6_002046"
     CDS             complement(4188..4979)
                     /gene="mazG"
                     /locus_tag="ACHOT6_002046"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFP9947267.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5050..5385)
                     /gene="mazF"
                     /locus_tag="ACHOT6_002047"
     CDS             complement(5050..5385)
                     /gene="mazF"
                     /locus_tag="ACHOT6_002047"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFP9947268.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5385..5633)
                     /gene="mazE"
                     /locus_tag="ACHOT6_002048"
     CDS             complement(5385..5633)
                     /gene="mazE"
                     /locus_tag="ACHOT6_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFP9947269.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5711..7945)
                     /gene="relA"
                     /locus_tag="ACHOT6_002049"
     CDS             complement(5711..7945)
                     /gene="relA"
                     /locus_tag="ACHOT6_002049"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFP9947270.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7993..9294)
                     /gene="rlmD"
                     /locus_tag="ACHOT6_002050"
     CDS             complement(7993..9294)
                     /gene="rlmD"
                     /locus_tag="ACHOT6_002050"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFP9947271.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPIFVPQLEALLPKVRACLGSLQAIRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNACVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9351..12107
                     /gene="barA"
                     /locus_tag="ACHOT6_002051"
     CDS             9351..12107
                     /gene="barA"
                     /locus_tag="ACHOT6_002051"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFP9947272.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12339..13679)
                     /gene="gudD"
                     /locus_tag="ACHOT6_002052"
     CDS             complement(12339..13679)
                     /gene="gudD"
                     /locus_tag="ACHOT6_002052"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFP9947273.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13700..15040)
                     /gene="gudX"
                     /locus_tag="ACHOT6_002053"
     CDS             complement(13700..15040)
                     /gene="gudX"
                     /locus_tag="ACHOT6_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFP9947274.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15042..16394)
                     /gene="gudP"
                     /locus_tag="ACHOT6_002054"
     CDS             complement(15042..16394)
                     /gene="gudP"
                     /locus_tag="ACHOT6_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFP9947275.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16828..17277)
                     /gene="yqcA"
                     /locus_tag="ACHOT6_002055"
     CDS             complement(16828..17277)
                     /gene="yqcA"
                     /locus_tag="ACHOT6_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFP9947276.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17295..18077)
                     /gene="truC"
                     /locus_tag="ACHOT6_002056"
     CDS             complement(17295..18077)
                     /gene="truC"
                     /locus_tag="ACHOT6_002056"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFP9947277.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18077..18406)
                     /gene="yqcC"
                     /locus_tag="ACHOT6_002057"
     CDS             complement(18077..18406)
                     /gene="yqcC"
                     /locus_tag="ACHOT6_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFP9947278.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19028..19573)
                     /gene="syd"
                     /locus_tag="ACHOT6_002058"
     CDS             complement(19028..19573)
                     /gene="syd"
                     /locus_tag="ACHOT6_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFP9947279.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19641..20489
                     /gene="queF"
                     /locus_tag="ACHOT6_002059"
     CDS             19641..20489
                     /gene="queF"
                     /locus_tag="ACHOT6_002059"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFP9947280.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20601..21965
                     /gene="ppnN"
                     /locus_tag="ACHOT6_002060"
     CDS             20601..21965
                     /gene="ppnN"
                     /locus_tag="ACHOT6_002060"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFP9947281.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22522..23811
                     /gene="sdaC"
                     /locus_tag="ACHOT6_002061"
     CDS             22522..23811
                     /gene="sdaC"
                     /locus_tag="ACHOT6_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFP9947282.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            23869..25236
                     /gene="sdaB"
                     /locus_tag="ACHOT6_002062"
     CDS             23869..25236
                     /gene="sdaB"
                     /locus_tag="ACHOT6_002062"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFP9947283.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25348..26103
                     /gene="xni"
                     /locus_tag="ACHOT6_002063"
     CDS             25348..26103
                     /gene="xni"
                     /locus_tag="ACHOT6_002063"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFP9947284.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26158..27306)
                     /gene="fucO"
                     /locus_tag="ACHOT6_002064"
     CDS             complement(26158..27306)
                     /gene="fucO"
                     /locus_tag="ACHOT6_002064"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFP9947285.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALNDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27334..27981)
                     /gene="fucA"
                     /locus_tag="ACHOT6_002065"
     CDS             complement(27334..27981)
                     /gene="fucA"
                     /locus_tag="ACHOT6_002065"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFP9947286.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6844 a         7503 c         6736 g         7057 t
ORIGIN      
        1 tataatgcgc tatttcatac taatgttcac tttcgtgtgt tcctttgttg cagcccaacc
       61 aactattgtc ccacaattac agcaacaagt tacggatctt acgagtagct taaactcaca
      121 agaaaagaaa gaactgacgc acaagttaga atctattttc aataataccc aagtgcaaat
      181 tgctgtatta attgttccca caaccaaaga cgagacaatt gaacaatatg ctacaagagt
      241 ttttgacaat tggcgtttag gagatgccaa acgtaatgat gggatactga tcattgttgc
      301 ctggtcagat cgtactgtcc gcatccaggt aggttatggg ctggaagaaa aggtaaccga
      361 tgctctggct ggagatatca tccgtagcaa catgataccc gcatttaaac aacaaaaatt
      421 tgctcaggga ttagagctag ctataaacgc tttgaataac caactcactt cacaacacca
      481 atatccgact aatccttcag aaagtgaatc agcgtcttcc agtgatcatt attactttgc
      541 tattttttgg gtatttgcag tgatgttctt ccctttctgg ttttttcatc aaggcagtaa
      601 tttttgtcgc gcatgtaaaa gtggagtctg tatttcagca atctatcttt tagatttatt
      661 cctgttctcc gataaaattt tttccattgc tgtattttcc ttctttttta ctttcaccat
      721 atttatggtc tttacctgtt tatgtgtact tcagaaaaga gcatcaggta aaagttatca
      781 ttcagacaac agcggttccg gtggaggctc aagtggcggc ggatttagcg gcggtggcgg
      841 ttcttctgga ggtggcggcg catccggccg ctggtaactc aaccataaaa aatgccagcc
      901 cggaggctgg catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt
      961 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
     1021 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
     1081 aacagccagg tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc
     1141 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
     1201 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
     1261 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
     1321 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
     1381 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
     1441 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
     1501 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
     1561 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
     1621 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
     1681 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
     1741 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
     1801 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
     1861 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1921 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1981 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     2041 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     2101 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     2161 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     2221 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     2281 aacgcgtacc cagggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     2341 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     2401 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     2461 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     2521 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     2581 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     2641 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     2701 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     2761 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     2821 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     2881 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2941 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     3001 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     3061 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     3121 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     3181 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     3241 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     3301 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     3361 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     3421 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     3481 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     3541 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     3601 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     3661 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     3721 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     3781 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     3841 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3901 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3961 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat
     4021 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     4081 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     4141 ttgtcattta aaaaatgaca caaatggcgc ttgaccgcgt aattccctta gagatcaatt
     4201 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     4261 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     4321 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     4381 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     4441 gcctcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     4501 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     4561 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     4621 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     4681 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     4741 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     4801 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     4861 gtttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4921 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacggtcgat ttgattcatt
     4981 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     5041 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     5101 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgatacttt ttacctgatc
     5161 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     5221 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     5281 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     5341 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     5401 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     5461 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     5521 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     5581 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat cataaccctt
     5641 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     5701 aaatttctgc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     5761 gtttacccag cacgcggccc agcacctgca ggttgtaaat ctcaatggtc atgtcgatgg
     5821 ttgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagcacgttc accttctcgt
     5881 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5941 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     6001 gcgcatggga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     6061 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     6121 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     6181 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     6241 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     6301 cgccaccaat cgccgccagc aactcgtcga catcattaaa gttgtaacgc ggcagcagat
     6361 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     6421 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     6481 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     6541 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     6601 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     6661 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     6721 agacatacac ccggtcgtca aagacctggc tgcgtacttc gtcgagcatt tcgccggaat
     6781 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     6841 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6901 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacctg
     6961 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacataat
     7021 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcatagc
     7081 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcaaagg
     7141 cgaggttctt tttctgcatt ttacgccaga tgctgtagat gtgtttcgga cgaccataca
     7201 cttcagcttt aacgccttca gctttcatct cagcgcgcag atgaccgaca aactcttcga
     7261 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     7321 tcgggtgcag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     7381 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acgcgttcat
     7441 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacca
     7501 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     7561 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     7621 cgtgaataag gttaacaacc gacttaccga cgctctcacg cagcacatct tcgctaacta
     7681 cgttggcatc agccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     7741 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     7801 gcccctgcgt ctgttgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     7861 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7921 tatgtgcact tcttaccgca accatcgtcc tctcctttag ggaccagacc tgccgaaatc
     7981 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     8041 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     8101 gctatcccga gccagcgttg caggattaca ggatacataa actatacgaa taggttccag
     8161 ttttataatt tgctgcataa cacctgcggc acctgctcgc gcggggtcca gcaacacttt
     8221 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcatgata
     8281 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     8341 cggaacacct tctacaccca ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     8401 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     8461 cgcacgcgct accatttttt ggttcacaca cgcattgacc tgaataaaat cgcgcgggct
     8521 aaaagttaag cgcaacccgt ttgagtcata ccagggcatc tcaccagaga cggtttcgag
     8581 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     8641 tttttcgcga tcagccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     8701 ggttgcctgt accagttcaa catgaccaag atggcgtata gcttgtaagc tgcccaggca
     8761 tgccctgact ttgggcagca atgcttcaag ttggggcaca aaaatggggc attgtttaac
     8821 gtcgacaatg tcactggagc ccgctttgcg aaagcccatc tgaagttgct gtgttttcgg
     8881 taagtagttc aaacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     8941 ttcagagact tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     9001 atccacgctg gcgtgttgtt gctgacagcc accgcatacg ccaaaatgag ggcagcgtgg
     9061 cgtttcgcgt tccgggctat cgcttaaccg gcgcacgact ttagcgcggg cgtactgttt
     9121 tttatcttca gtaacagtaa cttccgcgtt ttcctgtggc agtaatccag gaataaataa
     9181 cgctttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     9241 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     9301 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     9361 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     9421 tgctgagtat ctttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     9481 ccggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     9541 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     9601 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     9661 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     9721 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     9781 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     9841 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     9901 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9961 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
    10021 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
    10081 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
    10141 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
    10201 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
    10261 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
    10321 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
    10381 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
    10441 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
    10501 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
    10561 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
    10621 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
    10681 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
    10741 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
    10801 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
    10861 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10921 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10981 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    11041 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    11101 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    11161 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    11221 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctacg cgcctgttgc
    11281 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    11341 gcaagctggc aatgacagtc atggcagttg atgacaaccc cgctaacctg aaacttatcg
    11401 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    11461 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgcctgaca
    11521 tggatggcat tcgggcctgc gagctcatcc accagctccc ccatcagcaa caaacgccgg
    11581 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    11641 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    11701 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    11761 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    11821 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    11881 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11941 atggcagttg cggctatagc ggtgtgccgc gtatgaagaa tctctgccaa ctgatcgaac
    12001 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    12061 acgagatgga taatgtagcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    12121 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    12181 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    12241 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    12301 aagccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    12361 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatc gtcacgcgcg
    12421 ccaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    12481 acacccagcc ccggtttttc cggcacctga accagaccgc ctttgatctc aaacggttct
    12541 ttggtcaagc gctgattgcc ttcctgccaa atccagtgcg tatcaatcgc ggtgatttta
    12601 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    12661 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    12721 tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    12781 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    12841 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12901 tctgcataag ccagcgaccc tttcaggtat ttaccaattt taatcgcttc gttcagcgac
    12961 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    13021 atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    13081 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    13141 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt
    13201 gtggctttgc gatcaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    13261 tgaccatcgc ccagcaggga tgccacgttt acccccagat gctgccccag cagatccagc
    13321 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    13381 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    13441 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    13501 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcaca
    13561 atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    13621 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    13681 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    13741 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgaca ggcagacgtt
    13801 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    13861 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13921 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13981 ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg cagccccagg
    14041 tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc gtccagaagt
    14101 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    14161 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgccga aactccgcca
    14221 taacttcacg accggagaaa ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    14281 catcattcag ccctttgcat agagaaatgg cttcatcaag tagccaggca ccgttgggat
    14341 caacggtaat ccgcgcatca gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    14401 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    14461 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    14521 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    14581 cgatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    14641 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    14701 ccgccacggc attaacgcgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    14761 cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc gcaacttcct
    14821 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    14881 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg cgggtgaaat
    14941 atgcgttatg tgccccacca atattaagca acatgctatc gtgcccggcg accggaatga
    15001 ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc cgcgacaggt
    15061 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattaag
    15121 gcatgaacac caacataaat cagcgcccca ttaaaggagc cagtcgtgcc aacgatataa
    15181 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    15241 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    15301 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    15361 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    15421 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc acccagcaca
    15481 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaatcgac
    15541 atcccgcgtg cctgcaccag ataaaccggg aaccaggtaa taaagaagta agtcagggcg
    15601 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagctgtttg
    15661 atctgccccc acttcacgct gaacggcact ttaactttag tgttttgctg atccatattg
    15721 atcagcgcgc cacccgcggc gatatactcc agctctttct gatttacccc cggatgttga
    15781 tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag accgcccata
    15841 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15901 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15961 gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    16021 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    16081 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    16141 tttgaaccaa aacggtccag caaccagcca ccagggatct gcccgataac ataagcccat
    16201 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    16261 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    16321 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    16381 cttaaagaac tcattgttgc actcctgaaa attcgcgtta gccacgctca ctctggactg
    16441 cgacatagcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    16501 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    16561 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    16621 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggtaaaaac
    16681 gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc cagtaaaaat
    16741 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    16801 aaacgacatt taccgctcgc tgacatatca ggacaacagc gtgccccact gttcgaccca
    16861 cggatttgat tccgtttccg gttccgggtt ctcactggcg tcaatcaaca gcatttcgcc
    16921 aacccgctga gcgctctgtt cctgcaacaa ggcatcgaac tgtttgccgc cattgcagaa
    16981 atttacataa ctgctgtcgc cgagcgcaat cacgccataa cgcagattcg gctggaagcc
    17041 cagactatct ttgattccct gaaagagcgg cacaatgcta tcaggaaggt cgccctgccc
    17101 ggtcgtggac gtaaccacca gaacatactt atcctgataa ggcagccagt cgcttaattc
    17161 aggatcttca aataccgttg ctttgtggcc ctgtgcggtc agaattgctt ctgcttcttc
    17221 cgccactaac agcgaatttc cgtacatggt gccgacaaaa ataccaattt ccgccatacc
    17281 gttttctccc tggattagga acttatctct ccatcctgac ccgatggcgc actgaactca
    17341 accctttcat tttcaggaag cagaccgcgc cagccaaatt gtgataacgc ctgcatccag
    17401 gtgtcgtcca ggcccgcgtg aatagtcagc ggctcgccag taaaaggatg cgtcaatgac
    17461 aactgactgg catgtagcat taaccgctgg aggccaaaat gctcagcacc gctgcgattc
    17521 tggcgtaaat cgccatgttt gctatcgcca ataatcggat gacgcaaatg ggcaagatgt
    17581 cggcgaagct gatgtttgcg tccggttttc ggctccagtt ccaccaggcc gtagcgcgtg
    17641 gtcgggtaac gtccggtcgc taccggcatt tctacggtcg ccagaccgcg ataatgcgtc
    17701 actgctggct gcgggccttt atcttcgcgg gcaaatttat cagcgatttt gtccagttct
    17761 tccaccagtg gataatccag caccgcttct tccatcaacc agccgcgcac aatcgcatgg
    17821 taacgtttct ggatttggtg ctgttcaaac tgttgtgcca gcagccgtcc ggcctcgctg
    17881 gataatccca tcaacaacac accagaagtg ggtcggtcca gacgatgagc agtaaaaaca
    17941 tgctgtccta tctggtcacg cacggtttgc atgaccacta ctttctcgtc gcgatccagc
    18001 cagctacggt gaaccagcca gccggagggt ttatttaccg caaccagcca ttcatcctga
    18061 tagagtattt ccagcattag ctcgcatcat ccgcaaaaag agcatccagt ttttccaact
    18121 cagccagaat aagcgcgcgt tgcggatggt ccgtcgccag cgccatttca taatagggtg
    18181 caacagcaaa agcgcccggt aacggctggt tgttatttaa caaatcgtgc attcgcggga
    18241 tcagcaccca ctgcaaccac tccagtggtt ccatggtgtc cataaagaac ggttgggtac
    18301 tattaaattg atgcggctgg ggttcatcgt ttcgccagtg ctgatgttca cgcagtaacg
    18361 cttcaagcgc ctgcaactgg agacgaacgc ggtcatgagt ggtcataaag caacctcaat
    18421 aagaaaaact gccgcgaaga atagcaggaa taaaaaaagg gagcactgta ttcacagcgc
    18481 tcccggttcg tttcgcagca ttccagctac ttttgttgct ccctgctcat ccttgacaac
    18541 ttttcctctg gccttgcggc caatcgttca tcctgaacta ttgcttcctg ctcacaccac
    18601 cccgatgtga tacttcatcc tgaagtgtcc ctggccttcc tgacccaccg aatcatcctg
    18661 accggttctc attctccatc ctggaggtgt cctttaacgc gtcctgcgtc atcctcttcg
    18721 cttcatccag aagcctttcc ctgaaacacc atcctggtgt gtcctgcaga agtgtcatca
    18781 tcctgatgtt cacttcgttg tctgactccc tgtcgacgaa gatagaatcg tctttttcgc
    18841 gaagtcttac aaggcgctta cagacaatgc cttaaggaaa atttcatatg aaagttaaga
    18901 attaacaatc aacattttga ttttaaatga aaataacgat tcttatcttt ttaagtattc
    18961 gcttatttcc tggcgatctc ctacagagtc tgtaagagat ctctcacaac cagatggggt
    19021 atttggatta cagaagaggc ttaagttgat taaggaattc cgcaagattt gaggctaaat
    19081 gcgtccgttt acgtgttccc agcgtctctt tgcacacttc accggagaga ttacacaccg
    19141 agatgacctc taactcttct tccagcgtcg cgataaatag cgtgggcggc agttttagcc
    19201 ttttctgagt caccagatga ccaatcagat tttcctgcac ccgacggaag tcgtcttcac
    19261 tccaggtttg cagcaatgtc agtttgatat cgccaaactg cgcgtgcata tccccggcaa
    19321 actgagtcgt ataaaaggta tgaattgtgg gttgtatcac aatatcaaaa gcgcgttcaa
    19381 ccgcgtttac attttgttcc ccggtaaacg gctggggttg ccagtatacg gcatcttcgg
    19441 tagttgaaat aatgcacggt gaaggaacac cgtacagttc ctcacttaac ggccagcttt
    19501 tatgctcttc atgccacgca tcgcagtagc gtgcagtaaa atctttcagg gcttgtgcgg
    19561 tcaaatcgtc cactaatttc tctcttcact taaaccagat acacttgtcg tttagtttat
    19621 ctggtttatg acggtgaaac atgtcttctt atgcaaacca tcaggcactt gcgggcctga
    19681 ctcttggaaa atcaaccgat taccgggata cctatgacgc cagcctactg caaggcgttc
    19741 cacgcagcct gaatcgcgac ccgctgggtc tgaaagcgga taacctgcct tttcacggta
    19801 cggatatctg gacgctgtat gaactttcct ggctgaatgc gaaaggtttg ccgcaggttg
    19861 ccgtcggtca cgttgaactt gattacacca gcgcaaatct gattgagtcg aagagtttta
    19921 agctctatct caacagtttt aaccagacgc gttttaataa ctgggatgag gtgcgccaga
    19981 cgctggagcg cgacttaagc acttgcgccc agggtaaggt tagcgtggcg ttatatcgtc
    20041 ttgatgaact ggaaggccag ccgataggtc attttaatgg cacttgcatt gatgaccagg
    20101 atatcactat cgataactat gaattcacta ctgactatct ggagaatgcc acctgtggtg
    20161 aaaaagtagt ggaagagacg cttgtcagcc acctgctgaa atcaaactgc ctgatcaccc
    20221 atcaaccaga ttggggttcg atccaaattc agtatcgtgg acgccaaatt gacagagaaa
    20281 aactgctgcg ttacctggtc tcattccgtc atcacaacga gttccacgaa cagtgcgtgg
    20341 aacgcatctt taatgacctg ttacgcttct gccagccaga aaaattgagc gtttacgcac
    20401 gttatacccg tcgtggcggt ctggacatta acccgtggcg cagtaatagc gattttgtcc
    20461 catcgaccac aagactggtt cggcaataaa ttttttctca attttgcgtg ctggattcac
    20521 gcagaaggtt gtgaaaggtc atcaggcagg gctattgtaa tcaaagggaa tgacgatatt
    20581 cgtcccataa ggagtttttc ttgattacac atattagccc gcttggctcc atggatatgt
    20641 tgtcgcagct ggaagtggat atgcttaaac gcaccgccag cagcgacctc tatcaactgt
    20701 ttcgcaactg ttcacttgcc gtactgaact ccggtagttt gaccgataac agcaaagaat
    20761 tgctgtctcg ttttgaaaat ttcgatatta acgtcctgcg ccgtgaacgc ggcgtaaagc
    20821 tggaactgat taatcccccg gaagaggctt ttgtcgatgg gcgaattatt cgcgctttgc
    20881 aggccaactt gttcgcggtt ctgcgagaca ttctcttcgt ttacgggcaa atccataaca
    20941 ccgttcgttt tcccaacctg aatctcgaca actccgtaca catcactaac ctggtctttt
    21001 ccatcttgcg taacgctcgc gcgctgcatg tgggtgaagc gccaaatatg gtggtctgct
    21061 ggggcggtca ctcaattaac gaaaatgagt atttgtatgc ccgtcgcgtc ggaaaccagc
    21121 tgggcctgcg tgagctgaat atctgcaccg gctgtggtcc gggagcgatg gaagcgccga
    21181 tgaaaggtgc tgcggtcgga cacgcgcagc agcgttacaa agacagtcgt tttattggta
    21241 tgacagagcc gtcgattatc gccgctgaac cgcctaaccc gctggtcaac gaattgatca
    21301 tcatgccaga tatcgaaaaa cgtctggaag cgtttgtccg tatcgctcac ggtatcatta
    21361 tcttccctgg cggtgtgggt acggcagaag agttgctgta tttgctggga attttaatga
    21421 acccggccaa caaagatcag gttttaccat tgatcctcac cggcccgaaa gagagcgccg
    21481 actacttccg cgtactggac gagtttgtcg tgcatacgct gggtgaaaac gcgcgccgcc
    21541 attaccgcat catcattgat gacgccgctg aagtcgctcg tcagatgaaa aaatcgatgc
    21601 cgctggtgaa agaaaatcgc cgcgacacag gcgatgccta cagctttaac tggtcaatgc
    21661 gcattgcgcc agatttgcaa atgccgtttg agccgtctca cgagaatatg gctaatctga
    21721 agctttaccc ggatcaaccc gttgaagtgc tagctgccga cctgcgccgt gcgttctccg
    21781 gtattgtggc gggtaacgta aaagaagtcg gtattcgcgc cattgaagag tttggtcctt
    21841 acaaaatcaa cggcgataaa gagattatgc gtcgtatgga cgacctgcta cagggttttg
    21901 ttgcccagca tcgtatgaag ttgccaggct cagcctacat cccttgctac gaaatctgca
    21961 cgtaatctcc cctcccccgg tgacgtttgt tgccgggttt tctcgttttt ggtcacttac
    22021 tcatcaactc atttcatttg ttatatgaat gtttcttacc accctcacgc gacaaatatc
    22081 atcacagtta atatgtcata caatttatgt tgcaacgcaa acgtttccct attttcataa
    22141 accgtatttt atcgctatga aaaagaaatt atcgccatga ttaactaaaa gtattgattt
    22201 tttcagttca acctacatat attgcgcgcc ccggaagaag tcagatgtcg tttaatgggc
    22261 aaatattgcc cttaaattct cttttacttt tgatttacag agtaaagcgt tgggataatc
    22321 tatcttccaa gtagattatt gtatttgaga tcaagatcac tgatagatac ataacttgtg
    22381 tgtatctttc cgccctcaaa ttattacggc ggtaaatgat taagccatcg ccgatagaca
    22441 gatttcattt ttacggtcag gcaccttccc gggctgaact ggctaaaagc tgaattattt
    22501 gcattcctcc aggagaaata gatggaaacg actcaaacca gcacgattgc gtcgaaagac
    22561 tctcgtagtg cctggcgcaa gacagacacc atgtggatgc tgggccttta cggcacggca
    22621 atcggcgcgg gcgtgctgtt cctgccaatc aacgccggtg ttggcggtat gatcccgctg
    22681 atcatcatgg ctatccttgc gttcccgatg acgttttttg ctcaccgcgg cctgactcgc
    22741 ttcgtactgt ctggtaaaaa cccgggcgaa gacatcaccg aggttgtaga agaacacttt
    22801 ggtattggcg caggtaaact gattaccctg ctctacttct tcgctatcta cccgatcctg
    22861 ctggtttata gcgtggcaat caccaataca gttgaaagct tcatgtctca ccagctgggt
    22921 atgacgccac cgccgcgtgc gattctgtcg ctgatcctga tcgtgggtat gatgaccatc
    22981 gttcgcttcg gtgagcagat gatcgttaaa gcgatgagta ttctggtgtt cccgtttgtt
    23041 ggcgtattga tgctgctggc tctgtacctg atcccgcagt ggaacggcgc agcactggaa
    23101 acgctgtctc tggacactgc atctgcaacc ggaaacggtc tgtggatgac cctgtggctg
    23161 gcaattccgg taatggtgtt ctcgttcaac cactctccga tcatctcttc tttcgccgtt
    23221 gcgaagcgtg aagagtacgg cgatatggca gaacagaaat gctcgaagat cctggcattc
    23281 gcacacatca tgatggtgct gaccgtaatg ttcttcgtct tcagctgtgt actgagcctg
    23341 actccggcag acctggctgc ggctaaagag cagaacatct cgattctgtc ttacctggct
    23401 aaccacttta acgcaccggt tatcgcgtgg atggctccga ttatcgcgat tatcgctatc
    23461 accaaatcct tcctcggcca ctacctgggc gcacgtgaag gcttcaacgg tatggtgatt
    23521 aaatctctgc gtggtaaagg taagtctatc gaaatcaaca agctgaaccg tatcactgcg
    23581 ctgttcatgc tggtaacgac ctggattgtt gccaccctga acccgagcat cctgggtatg
    23641 attgaaaccc tgggcggccc aatcatcgcg atgatcctgt tcctgatgcc gatgtacgca
    23701 attcagaaag taccggcaat gcgtaagtac agcggtcaca tcagcaacgt attcgttgtc
    23761 gtgatgggtc tgattgcaat ctccgcaatc ttctactctc tgttcagcta agtcctttcg
    23821 cgccgctttc gggcggcgct tcctccgttt taacgcgatg tatttcctat gattagcgta
    23881 ttcgatattt tcaaaatcgg cattggccct tccagttctc ataccgttgg accaatgaaa
    23941 gcgggtaaac aatttaccga cgatctgatt gcccgtaatc tgcttaaaga cgtgacccgc
    24001 gtggtggttg acgtgtacgg ctcgctctct ctgaccggta aaggccacca cactgatatc
    24061 gccattatta tgggcctggc gggtaacctg ccggataccg tggatatcga ttccatccct
    24121 ggttttattc aggatgtgaa tactcatggt cgcctgatgc tggcaaacgg tcagcatgaa
    24181 gtggagttcc cggttgatca gtgcatgaac ttccatgcag acaacctttc tctgcatgaa
    24241 aacggtatgc gcattaccgc gctggcgggc gataaagtcg tttacagcca gacttactac
    24301 tctattggcg gtggctttat cgttgatgaa gagcattttg gccagcagaa tagcgcaccg
    24361 gttgaagttc cttatccgta cagttcagca gccgatctgc aaaaacattg tcaggaaacc
    24421 gggctgtcac tctctggcct gatgatgaaa aatgagctgg cgctgcacag caaagaagag
    24481 ctggaacagc acctggcgaa cgtatgggaa gtcatgcgtg gcggtattga gcgcggtatt
    24541 tccaccgaag gcgtgttgcc tggcaaactg cgcgttccac gccgtgctgc ggcactacgc
    24601 cggatgctgg tcagccagga taaaaccacc actgacccga tggcggttgt tgactggatc
    24661 aacatgtttg cactggcagt gaacgaagag aacgctgctg gcggacgcgt ggtgactgcg
    24721 ccgactaacg gtgcgtgcgg gattatcccg gcagtgctgg cgtactacga caagtttatc
    24781 cgcgaagtga acgctaactc actggctcgt tacctgctgg tagccagtgc cattggttct
    24841 ctttataaga tgaacgcgtc aatttctggt gctgaagtcg gctgccaggg tgaagttggt
    24901 gtggcgtgct caatggcggc ggctggtctg gcagaactgt taggtgcaag cccggcgcag
    24961 gtgtgcatcg cggcggaaat cgccatggag cacaacctcg gtctgacgtg tgacccggtc
    25021 gccggacagg tccaggtgcc atgcatcgag cgtaacgcca ttgcggcagt aaaagcggtg
    25081 aacgccgcac gtatggcgct acgccgtacc agcgagccgc gcgtctgcct cgataaagtt
    25141 atcgaaacca tgtacgaaac aggtaaagat atgaacgcca agtaccgcga aacctctcgc
    25201 ggcggcctgg caatgaagat cgttgcctgc gattaatcgc tctccagagg cctcgttttg
    25261 cgaggcctct tcccgatttc tcatccagcc gtagcctgtt ccggcatcga atgttaccct
    25321 tatcgcctga tctttaaggg ggttatcgtg gctgttcatt tgcttattgt cgatgcactg
    25381 aatcttattc gtcgcattca tgccgttcag ggatcgccct gtgtcgaaac ctgccagcat
    25441 gcgctcgatc agctcattat gcacagccag ccaacccacg cggtcgccgt ttttgatgat
    25501 gaaaaccgca gtagcggctg gcgtcatcag cgtttaccag attacaaagc gggtcgaccg
    25561 ccaatgccgg aagagttgca cgacgagatg cctgcattac gcgccgcctt tgagcaacgc
    25621 ggcgtcccgt gctggtcaac cagcggcaac gaagccgatg acttagccgc cacgctggcg
    25681 gtcaaagtga cacaggccgg gcatcaggca acgattgttt cgacagataa aggctactgt
    25741 cagttacttt caccgacatt acgtattcgt gattacttcc agaaacgttg gctggatgcg
    25801 ccatttatcg ataaagaatt tggcgttcaa ccgcagcagt tgcccgatta ctggggactt
    25861 gcggggatca gcagttcaaa ggtaccgggt gttgcgggaa tcggaccaaa aagcgccacg
    25921 cagctgctgg tcgagtttca gagtctggaa gggatatatg agaatctgga tgcggttgcc
    25981 gaaaagtggc gcaaaaaatt agaaacccat aaagagatgg cgtttctgtg ccgcgatatt
    26041 gcccgcttac aaaccgattt gcatatcgac ggcaatttac agcaattgcg gttggtacgg
    26101 taacggcgag ccggatacgc cgcaaacgtc gtatccggca ttatcacatc agcgcattta
    26161 ccaggcggta tggtaaagct ctacaatatc ctcaagcgtt gcttcacgcg ggttgccacc
    26221 ggtacaaaca tcattcagtg ccgcctgcgc cagtgccgga atgtcttcct tgcgtacccc
    26281 aacatcacgc aaatgtggcg gaataccgac atcacggttg agagcaaaca ccgcttcaac
    26341 agcggcatta cgcgcctctt ccaggctcat accttccact ttcacgccca taacgcgcgc
    26401 gatatcgcgg tacttctcac cggtaaagtc agcgttatag cgcatgacat gcggtagcag
    26461 gatggcgttt gcaacaccgt gtggagtgtt ataaaacgcg cccagtggat gcgccatacc
    26521 atgcaccaac cctaacccaa cattcgagaa gcccataccc gcaacatact gcccgagcgc
    26581 catttcttct ccggcatcct tatcaccagc aaccgatcct cgcagcgccc cagcaatgat
    26641 ttcaatcgct ttaatgtgca gtgcatcggt tagcgcccac gcgccacggg taatataccc
    26701 ctcaatagca tgagtgagcg catcgacacc cgtcgcagct ttcagcgctg gaggcatacc
    26761 atccatcatg tcagcgtcaa taaacgccac ctgcgggata tcatgcggat caacgcaaac
    26821 aaacttgcgc cgtttttctt cgtcagtgat cacgtagtta atggtcactt ctgccgcagt
    26881 gcctgctgtg gtggggattg ccagaatcgg tacactgggt ttattggtcg gggaaagtcc
    26941 ttccaggcta cgcacatcgg caaactccgg gttgttgctg ataatgccaa tcgctttaca
    27001 agtatcctgt ggagaaccac caccaatagc gatcaggtaa tccgcgccgc tattctggaa
    27061 tacaccgagc ccttctttga cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat
    27121 cgcccatgcc agccctgcag catccatctt atcggtcact ttcgccacca cgccgcattg
    27181 caccagcgtt ttatcggtga cgatcagcgc cttctgataa ccacggcgtt tcacctcatc
    27241 ggttaaagcc ccaacagcac cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt
    27301 agccatcatc cttctccttg ttgctttacg aaattactct tcaattcgta acccataggt
    27361 tttgaatttc tccagcacta cggcaatctc ttcatcgctc agcactggca ccgggtccgt
    27421 aatcgccagg gtcgtcaggt aaagttgcgc cagcacttca acttcatgcg ccagccataa
    27481 cgctttttcc agattcacct cacaagcgat aagcccatga tgttgtaaca aagttgcctt
    27541 acgatttttg agagcctgcg caacatgttc agaaagttca cgtgttccaa aggtcgcata
    27601 aggcgcgcaa ggaatagaat taccgccagc cgccgcaatc atgtagtgaa tagcggggat
    27661 cgatcggtta agaatggaaa ctgccgtgca atgaacggca tgattgtgaa caaccgcgtt
    27721 ggcatccggt ctgctttgat aggccgccat atggaaacgc cattcgcttg aggggagctt
    27781 tccttcctca tgtttaccgt tgccatcaat aaagacaata tgcgactccg tcagtttttc
    27841 atatggaatg cctgtaggcg taatcagcat cccatcctga taacgtacac tgacgttccc
    27901 cgctgtcccc tggttcagtc ccaggcgggt catttccagg caagtgtcaa taatctgacg
    27961 agcaagttta tttcgttcca ttagctacct ctctctgatt caaaacaggg caataatgtt
    28021 gttcctttca cactattgaa ttagccgttt aattacccac catcttcttc ctgattaacc
    28081 agaaagaaat tcacaagctt atattttgtg acctggttca actaatcaca gtaaataact
//
LOCUS       DAZKIV010000049        27540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZKIV010000049 DAZKIV010000000
VERSION     DAZKIV010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.049"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..884)
                     /gene="ycfZ"
                     /locus_tag="ACHOT6_002066"
     CDS             complement(96..884)
                     /gene="ycfZ"
                     /locus_tag="ACHOT6_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFP9947287.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(881..1342)
                     /locus_tag="ACHOT6_002067"
     CDS             complement(881..1342)
                     /locus_tag="ACHOT6_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFP9947288.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            complement(1400..2446)
                     /gene="potD"
                     /locus_tag="ACHOT6_002068"
     CDS             complement(1400..2446)
                     /gene="potD"
                     /locus_tag="ACHOT6_002068"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFP9947289.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(2443..3237)
                     /gene="potC"
                     /locus_tag="ACHOT6_002069"
     CDS             complement(2443..3237)
                     /gene="potC"
                     /locus_tag="ACHOT6_002069"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFP9947290.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            complement(3234..4091)
                     /gene="potB"
                     /locus_tag="ACHOT6_002070"
     CDS             complement(3234..4091)
                     /gene="potB"
                     /locus_tag="ACHOT6_002070"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFP9947291.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNDFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            complement(4075..5211)
                     /gene="potA"
                     /locus_tag="ACHOT6_002071"
     CDS             complement(4075..5211)
                     /gene="potA"
                     /locus_tag="ACHOT6_002071"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFP9947292.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            5461..6687
                     /gene="pepT"
                     /locus_tag="ACHOT6_002072"
     CDS             5461..6687
                     /gene="pepT"
                     /locus_tag="ACHOT6_002072"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFP9947293.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGNIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            complement(6736..7857)
                     /gene="roxA"
                     /locus_tag="ACHOT6_002073"
                     /gene_synonym="ycfD"
     CDS             complement(6736..7857)
                     /gene="roxA"
                     /locus_tag="ACHOT6_002073"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFP9947294.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            8106..9335
                     /locus_tag="ACHOT6_002074"
     CDS             8106..9335
                     /locus_tag="ACHOT6_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9947295.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLSDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWMERLGI
                     LAGTHENVTARPAARRK"
     gene            9691..9879
                     /locus_tag="ACHOT6_002075"
     CDS             9691..9879
                     /locus_tag="ACHOT6_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9947296.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            9937..10437
                     /locus_tag="ACHOT6_002076"
                     /pseudo
     CDS             9937..10437
                     /locus_tag="ACHOT6_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309603.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10380..10559)
                     /locus_tag="ACHOT6_002077"
     CDS             complement(10380..10559)
                     /locus_tag="ACHOT6_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9947297.1"
                     /translation="MATKAVNAKSQTVAARVPHEVMNNVEAVKMPGESTGQFVTAALK
                     REVEYRQRRKAKEPE"
     gene            10759..10965
                     /locus_tag="ACHOT6_002078"
     CDS             10759..10965
                     /locus_tag="ACHOT6_002078"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9947298.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFIALSTAQPRVITIEATSEQEA
                     RQQSPAGCVMVFAARIRQEVCHVQ"
     gene            10955..11419
                     /locus_tag="ACHOT6_002079"
     CDS             10955..11419
                     /locus_tag="ACHOT6_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947299.1"
                     /translation="MCNNIRPDAAAEAIITLMHALIDISVIADMAHKHATSETEYAGA
                     FVPHSLAVMQLSADMALNEAKAILIADCENGGVMRDDRFNSLKQEFSGVPDDAADALS
                     SMPELIIAAFFLLSTREYKSTGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            11412..11645
                     /locus_tag="ACHOT6_002080"
     CDS             11412..11645
                     /locus_tag="ACHOT6_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947300.1"
                     /translation="MRDIYHQTIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLDPVIMELQEIDLLHDRYKEQHTGE"
     gene            11651..11950
                     /locus_tag="ACHOT6_002081"
     CDS             11651..11950
                     /locus_tag="ACHOT6_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9947301.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            11947..13347
                     /locus_tag="ACHOT6_002082"
     CDS             11947..13347
                     /locus_tag="ACHOT6_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase RepA family protein"
                     /protein_id="HFP9947302.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPAEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVIRIDGDDVAPL"
     gene            13548..13799
                     /locus_tag="ACHOT6_002083"
     CDS             13548..13799
                     /locus_tag="ACHOT6_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947303.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            13796..14206
                     /locus_tag="ACHOT6_002084"
     CDS             13796..14206
                     /locus_tag="ACHOT6_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9947304.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            14217..14489
                     /locus_tag="ACHOT6_002085"
     CDS             14217..14489
                     /locus_tag="ACHOT6_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9947305.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            14446..14574
                     /locus_tag="ACHOT6_002086"
     CDS             14446..14574
                     /locus_tag="ACHOT6_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFP9947306.1"
                     /translation="MVLVMKYGAITRTANQTAGEIRLFLHQLKRYGALPGFRHGQA"
     gene            14616..14840
                     /locus_tag="ACHOT6_002087"
     CDS             14616..14840
                     /locus_tag="ACHOT6_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947307.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMSINDYRAFLETGALIEKDQHG
                     FVRCALSGEMLAVNPEQIDALIEFLKEIRD"
     gene            15092..15298
                     /locus_tag="ACHOT6_002088"
     CDS             15092..15298
                     /locus_tag="ACHOT6_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947308.1"
                     /translation="MAEEKKGGVSVYISPDIVKALKERHQQNVKAGIAAGLDPLAMVE
                     PSTGWQVRAYLRAALGMNQTHGGE"
     gene            15298..16353
                     /locus_tag="ACHOT6_002089"
     CDS             15298..16353
                     /locus_tag="ACHOT6_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9947309.1"
                     /translation="MAGKATALNTNQLFAYLNRGDIAEFKFSPLFTTLFFPNVATFNT
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNLIEGEGIERYE
                     IDWKIPESNIIEQADGKKWSELDKETHDPIYDIELYADQGNCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAELACKNLGEVVSFKGYLGDIALVVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMFYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            16366..16701
                     /locus_tag="ACHOT6_002090"
     CDS             16366..16701
                     /locus_tag="ACHOT6_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9947310.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLAENGEFK
                     PWDGQESGKAVYLTSYPVDTSTQKSAQCYKTGIFNIAAVNWPESADTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            16714..17127
                     /locus_tag="ACHOT6_002091"
     CDS             16714..17127
                     /locus_tag="ACHOT6_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HFP9947311.1"
                     /translation="MATNESIMTLPLASKFKAEARAMADRGLSTYEAVYQLNSLEEKD
                     KPRADAIMALHESDDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQAVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            17333..17875
                     /locus_tag="ACHOT6_002092"
     CDS             17333..17875
                     /locus_tag="ACHOT6_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9947312.1"
                     /translation="MKSHLMNKKNMAKSCRVSATAFDKWGVTPVERKGREAFYDVASV
                     IENRVNNAISQLINDKGEIDDDELLRVRIRLLTAQAEAQELKNDRDRGDVIDTEFCLY
                     ALSKLASQISSIMDILPLTMQRSFPQITPAMLDRLKREVVKVCNASARVADNLPQILA
                     DYLKETTGNVPEKLLQKKGE"
     gene            18131..18412
                     /locus_tag="ACHOT6_002093"
     CDS             18131..18412
                     /locus_tag="ACHOT6_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947313.1"
                     /translation="MTEAELLGLIRRVAGISQQHDEQATQPDSVTAENYVRVVAEVMR
                     RDGIELNGVDMRNIRNRVLEMLAYNRRVELYREKEKITYHWKKPEWLRR"
     gene            complement(19014..20474)
                     /gene="phoQ"
                     /locus_tag="ACHOT6_002094"
     CDS             complement(19014..20474)
                     /gene="phoQ"
                     /locus_tag="ACHOT6_002094"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFP9947314.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(20474..21145)
                     /gene="phoP"
                     /locus_tag="ACHOT6_002095"
     CDS             complement(20474..21145)
                     /gene="phoP"
                     /locus_tag="ACHOT6_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFP9947315.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     FFELR"
     gene            complement(21313..22683)
                     /gene="purB"
                     /locus_tag="ACHOT6_002096"
     CDS             complement(21313..22683)
                     /gene="purB"
                     /locus_tag="ACHOT6_002096"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFP9947316.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            complement(22687..23328)
                     /gene="hflD"
                     /locus_tag="ACHOT6_002097"
     CDS             complement(22687..23328)
                     /gene="hflD"
                     /locus_tag="ACHOT6_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFP9947317.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            complement(23364..24470)
                     /gene="mnmA"
                     /locus_tag="ACHOT6_002098"
                     /gene_synonym="trmU"
     CDS             complement(23364..24470)
                     /gene="mnmA"
                     /locus_tag="ACHOT6_002098"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFP9947318.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            complement(24524..24985)
                     /gene="nudJ"
                     /locus_tag="ACHOT6_002099"
     CDS             complement(24524..24985)
                     /gene="nudJ"
                     /locus_tag="ACHOT6_002099"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFP9947319.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            complement(24995..25648)
                     /gene="rluE"
                     /locus_tag="ACHOT6_002100"
     CDS             complement(24995..25648)
                     /gene="rluE"
                     /locus_tag="ACHOT6_002100"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFP9947320.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            25820..27070
                     /gene="icd"
                     /locus_tag="ACHOT6_002101"
     CDS             25820..27070
                     /gene="icd"
                     /locus_tag="ACHOT6_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFP9947321.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSLTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            complement(27173..27496)
                     /gene="iraM"
                     /locus_tag="ACHOT6_002102"
     CDS             complement(27173..27496)
                     /gene="iraM"
                     /locus_tag="ACHOT6_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFP9947322.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGERKITNKCLHNSCIIKIC
                     PYGLK"
BASE COUNT         7277 a         6624 c         7036 g         6603 t
ORIGIN      
        1 aggggacgca attaattgaa tttgcgggtt ttgtgggccg gataaggcgt tcgcgcccca
       61 tccggcataa tacacgcgca cttttccggc ctcttttatc gtttactcgt caataacaat
      121 gcggcaataa acgtacagac accaattagc cctgcccaga gtggcgatat tctggtaatc
      181 gcaaagctat tatcgatata caaacataaa gcctgataac ccatattggc aatagtcatc
      241 gccactgcaa attttaacgc ataaataaaa cgagtcgtgt agcaaaagat ccaggaaaat
      301 aagataaacg tcgcagccca ggcgataaaa cctaatgtgt tggtgctgcc ggggggagca
      361 ttttcatgct gttgtcctgg atgcggataa tacgtcagtg gcggcgtcgg ttgttttaat
      421 atctgtgctt gtagaacagc caatgcatta ttgattccct gataccagag atctgcattt
      481 aaataagcca gcgtgtgatt ttctatcaga aggccgagat gtttatcgtc cagaccactt
      541 tcaacctggc cgttggtgat cataattaac cgtggttcgc gggtagtaaa tagtaacaga
      601 acacttcctt ttacattact caatgaagat gacgcatgcg ccagcatcgc tttaccattt
      661 tgtctgagac tggcttcatt attaatgtcg ggtaaaatca caataaacgg tgcaattccc
      721 gtttgttggc gaaaaagctc ggctttacgt gcgatctctt cagcatccgg tacagtaccg
      781 gtaacatccg taaaaagtgt cttcattatt ggaatcaatg gtttactggt tgctaccaga
      841 ggcaggagta tcagtaaact gagcagtata atgaattttt tcatgggcgg gacttccgtt
      901 tcctgcgaga ttttttggtg gaggggagcg tgattatcag accgacacta ccgaaaataa
      961 tgcctgctat gccaacaaag ccccatttaa aaaaattaac ccagggattg tttatttcgg
     1021 cttgttctgg atgccctggc ggataataga cattgactct gtcaccttcg ctatcttcat
     1081 aaaaatcact accgatattc gaattgaaaa ttagcgtgta atctggcgta gggcgaaagg
     1141 caaccactgg ataccaggaa ccattatcat cgacgtcgtt tgagtgattg ttgtaccaaa
     1201 cggcatcaac aatgattcct tctgtatgaa ccgcgctttt ggtaaaagta aaatggtaat
     1261 aaaagatgat tgcagaaata acgagcaata tcagaccgat accgagaaaa gcgcgggata
     1321 acactttact gtcctgactc attagccaca tccttgctaa ataaaaaacg ggcggtaatg
     1381 ccaccgcccg cttgctgaat taacgtcctg ctttcagctt ctgataatac tcttcataaa
     1441 tgctgctggc tgcgccaacg tcattctgcc attcaccatt tttaatggtt tcagcatccg
     1501 ggtagagcgt tttatcgttc gccacttccg ggcttaacag cttacgcgcc gcaaggtttg
     1561 gcgttggata accgatagtt tcggcaacct gttttgccac atccgggcgc agcaggaagt
     1621 tgatcaattt cagcgcgcct tctttgtttt tggcatttgc cgggatcgcc aggctgtcca
     1681 tccagaaaat gccgccttct ttcggccaca ccacgtcaat tggcgtaccc gcctggcgtg
     1741 caacgaaagc agagccgttc cagatcatgc cgaggttaac ttcgccttcc atgtacgggt
     1801 tagccgggtt atcggagtta aacgctgcga cgtttggcat cagttttttc agctcgttat
     1861 atgcagcttc aatctctttc ggatcggtgg tgttaccgga gtagcccagt ttgcgcagcg
     1921 ccatctggaa cacttcacgg gcatcgtcgg tcagcagcag gctacctttg tactctggct
     1981 tccacagatc tgcccaactg gtgacagatt taggatccac cgcatcaccg ttaacgccaa
     2041 tcgccgtcgc accccagata tacggaatgg agtagtcgtt attcgggtca aacggcttgt
     2101 tgagcatgtc tggatcgaga ttgctgaagt tcgttaactt cgacttgtcg atcttctgga
     2161 tcatcccttc tttacgcatt ttatcgacgt aataggttga aggaaccacc aggtcatagg
     2221 caccgtcttt gtacgttttc agcttggcgt acatggtttc gttcgactcg taagtcgaat
     2281 agataacctt aataccggtt tctttggtga actgttcaag cagtcctggc ggcacgtact
     2341 cggtccagtt gtagaaatac agcgtgttgt tgtcatcggc gtgagcggcg ctcatgccca
     2401 gtgccagagc acccgccgcg agcaggtggc gtgaccattt tttcatttaa cgtcccctgt
     2461 gttacctttc gttttatcac gagcaataag ctggctggca ataaccatca ccagcgacag
     2521 caccagtaat atggttgcca gcgcgttaac ttccggcgat acgccgactt tgaccatcga
     2581 ataaattttt aacggcagaa tttcataact tggtccggtg acgaacgaag aaactaccac
     2641 atcgtccatc gacagggtaa agcttaacac ccagcccgcc gccaccgcag gcattgccag
     2701 tggcagaatg atttttcgca gaatggtaaa ttcactggca ccgagatctt tcgccgcttc
     2761 cagcatccgc acgtcaaaac ctttcaggcg cgaatacacc gtcaccacca caaacggcag
     2821 gcagaaggtg atatgcgaga acagcagcga ccagaagcca agctgaatac ccagcagcat
     2881 aaacagcacc agcagagaaa tcgccatcac gatatctggt gacatcatca ccacaaacag
     2941 cattccgcta acgaacggct taccacgaaa gcgataacgg tacagcgcaa ctgccgtcag
     3001 tgaaccgata agcgtagcaa atgtcgccga aaacaccgcc attgttagtg aatgctgcgc
     3061 tgcctgtaac aggctgtcgt tgttcatcag caggctatac catttggtgg taaaaccctg
     3121 ccagttgatg ccaaagcgcg agctgttaaa ggagttcaca atcaaaataa tgattgggat
     3181 atacaggtac gcgtagatag cggtcataaa accgccgcga agcagtcgac cgatcattcg
     3241 agttccacct ttttattcag cagacgagaa gcgcgccagt aaaccagcaa catcaggccc
     3301 attacgatag tcagcgtaat gctggtggct gcaccaaacg gccagtcacg aatattgagg
     3361 aactggacct tgatgacgtt accgatcagc aggtttttcg caccgcccat caggtcggat
     3421 acatagaaca ggcccatcgc tggcagcatc accagcagac atccggcaat aattcccggc
     3481 atcgtcagtg gaataatgat acggataaaa gtctgtaact tgctggcacc gagatcgcgc
     3541 gccgcctcaa gcaacggctt atccagtttt tcgatactgg agtacagcgg catcaccata
     3601 aacggtagca gaatgtaaac cagaccgata atcaccgcac tgggcgtgaa catgatacga
     3661 attggtgtgt cgataacgcc cagccagagc aaaaaatcgt tgagatagcc tttggtgctg
     3721 aggaaaattt tcagcccgta gatgcgaatc agtgagttgg tccagaaggg aacaatcagc
     3781 agaaacagca gcagcggacg caccttgtgt ggtagcttcg ccagaaacca ggcaaacggg
     3841 tagcccagca ccaggcaggc gagggtggcg atcagcgcca tattcagcga gtgcaatagc
     3901 acttcaaaat agagcggatc gagcagacgc gtgtagttat ccagcgtaaa gaccattttg
     3961 acgaaactgg cgtcgtcgcg ggtcaaaaag ctggtgccaa tgatcatcag gttgggcaga
     4021 aagacaaaca acacaagcca accgacaata gtgacaatca ctacattctg gaacttactt
     4081 gtgttcttca tcagccagta cgacctccca gctttctacc caattaatgg ccattttttg
     4141 gtcgagagag tggtcaaagt cagggtcgtc ttcattgaag aattcgctga ccatcaccat
     4201 cttgccattt tccagttcaa caaccgactc cagcgtcatg cctttgtagt tacgctcacg
     4261 aacgtaacca atcagccctt cagcgtggtt gtcgtcgtta atctcttcaa cacgtaagtc
     4321 ttccgggcgc agcagaacat gcagtttttg ccccggttca acggcgaagt taacgtagat
     4381 attacattcg cggccttcaa cgttggcgcg tacgcgctgc tcgtctagac gttcgatgac
     4441 agtggcgtta aacatattga tttcgccaat gaagccggca acaaacaggt ttttcggctc
     4501 ttcgtaaatt tcgcgcggtg taccatcttg ctcaatacga ccctcgcgca tcaccacaat
     4561 cctgtctgac atggtgagcg cttcttcctg atcgtgagtg acaaagacga aggtaatacc
     4621 aagcttacgc tgtaacgctt tcagctcgtt ctgcatttgc ttacgcagtt tgtaatccag
     4681 cgctgagagc gactcatcca gcaacaacag acgaggcttg ttaaccaccg cgcgagcaat
     4741 ggcgacgcgt tgctgttgac caccagagag ctgatgcggt ttacgttgag cgaaggtttc
     4801 cagttgcacc atccgcaggg cttccatcac gcggggcgta atttcagcag cgggggtttt
     4861 ttgcatgcgc aacccaaagg ccacattttc gaacacggtc atgtggggga aaagtgcgta
     4921 gctttggaaa acagtgttca catagcggtt ttccgccgga acgtgggtga tgtcctcgtt
     4981 atccagcatg atgcgtccgg aatcaacagt ttccagacct gcaatcaggc gaagaacggt
     5041 tgttttaccg cagccagaag ggccaagcag cgtgaggaac tcgccattgt tgatagtcag
     5101 atccagctgg ggaatgacct ctttaccatc aaagcatttg cgaattcccg ccaattgcac
     5161 cagcggtgaa agcgaactcg gttgtttatt caatttttta ctctgtccca tgtaaacgca
     5221 acggatggct taccgatgcg gggtttgtgg ttaaccacct tggtgactct taatgagggc
     5281 ggtaattcta cggcaaaccg cttgaatcgc caatctttgt tgtgaattac tggcttagct
     5341 ttatattcat taaggtaatg ctgataaata ttcccgcttg caggggtaaa agtgacctga
     5401 cgcaatattt gtcttttctt gcttcttaat aatgttgtca caaaaagtga gggtgactac
     5461 atggataaac tacttgagcg atttttgaac tacgtgtctc tggataccca atcaaaagca
     5521 ggggtgagac aggttcccag cacggaaggc caatggaagt tattgcatct gctgaaagag
     5581 cagctcgaag agatggggct tatcaatgtg accttaagtg agaagggcac tttgatggcg
     5641 acgttaccgg ctaacgtccc tggcaatatc ccggcgattg gctttatttc tcatgtggat
     5701 acctcaccgg attgcagcgg caaaaatgtg aatccgcaaa ttgttgaaaa ctatcgcggt
     5761 ggcgatattg cgctgggtat cggcgatgaa gttttatcac cggttatgtt cccggtgctg
     5821 catcagctac tgggtcagac gctgattact accgatggta aaaccttgtt aggtgccgat
     5881 gacaaagccg gtattgcaga aatcatgacc gcgctggcgg tattgcaaca gaaaaacatt
     5941 ccgcatggtg atattcgcgt cgcctttacc ccggatgaag aagtgggcaa aggggcgaaa
     6001 cattttgatg ttgacgcctt cgatgcccgc tgggcttaca ctgttgatgg tggtggcgta
     6061 ggcgaactgg agtttgaaaa cttcaacgcc gcgtcggtca atatcaaaat tgtcggtaac
     6121 aatgttcacc cgggcacggc gaaaggagtg atggtaaatg cgctgtcgct ggcggcacgt
     6181 attcatgcgg aagttccggc ggatgaaagc ccggaaatga cagaaggcta tgaaggtttc
     6241 tatcacctgg cgagcatgaa aggcaccgtt gaacgggccg atatgcatta catcatccgt
     6301 gatttcgacc gtaaacagtt tgaagcgcgt aaacgtaaaa tgatggagat cgccaaaaaa
     6361 gtgggcaaag ggttacatcc tgattgctac attgaactgg tgattgaaga cagttactac
     6421 aatatgcgcg agaaagtggt tgagcatccg catattctcg atatcgccca gcaggcgatg
     6481 cgtgactgcg atattgaacc ggaactgaaa ccgatccgcg gtggtaccga cggcgcgcag
     6541 ttgtcgttta tgggattacc gtgcccgaac ctgttcactg gcggttacaa ctatcatggt
     6601 aagcatgagt ttgtgactct ggaaggtatg gaaaaagcgg tgcaggtgat cgtccgtatt
     6661 gcagagttaa cggcgcaacg gaagtaagcg aaaagggatg cggcatgtga tgccgcatcc
     6721 ggcttaaatc taaacttacc cttcgaagaa ccagtatccg ctattgacca gcgccgcgag
     6781 catcgcgagg aatgacggat cttccagcgc atcgccaaaa ttctccgcgg tcagcgcaat
     6841 gttgctggcg agtgcatcca gtgccggacg gtgcggggaa tcgatcttct caccattggc
     6901 atacacgtcg tcgccaatgc gcaatacgcg cagaccaccc aggcgcacca gcacttcacc
     6961 ttgtttcagc gcatcgtaga tttcatctgg ctgataaggc ggttccggcg gcgcgatatc
     7021 cagttcatga cgtgactggg atataaactc gccaaaccat tgcttaaagt gttccggctg
     7081 gttgatcaat tcgagcatca tctcacgcag tttatccatc tcttgcggca gaacgtccgc
     7141 aggatgagcg cgaggtggaa catccggatc gctgtagtag ttgccgccca gttcacgttg
     7201 cagcacataa tcggcaaatc cgctaattaa ttcccgcgta tttggcgcgc gaaaacccac
     7261 ggaatagttc atcgcatttt ccagcgcgta gccttcatgc gggaatcctg gcggaatata
     7321 aagaatatcg ccaggctcca gctcttcatc gatgatggct tcgaacggat cgacctgtaa
     7381 cagatccggg tgcggacagt gctgtttcat ttgcagcttt tcgcccactc gccagcgacg
     7441 acgaccggta ccctgaatga taaacacgtc gtactgatcg agatgcgggc cgacgccgcc
     7501 gcccggtaca gaaaaagaga tcatcagatc atcaatacgc cagtccggta gttcacggaa
     7561 cggtcgcatc agcgcggcgg tcggctcatg ccagtggttc actgcctgta ccagtaatga
     7621 ccagttggtt tcaccgagat gatcgtagct ttcgaacggg ccgtggctga cctgccattt
     7681 gccatcctgg tgactgacca gtcgactgtc aacttcgctt tccatcgcca gacccgccaa
     7741 ctcgtctgga gagatcgggt caataaaatt attaaagccg cgttttaaca ccaccgggcg
     7801 tttctgccag tgacgttcaa gaaaatcggg ccagttaaga gtgagttggt attccatgtt
     7861 aagcatccac aggctggtat ctgcaaccga ttataacgga tgcttaacgt aatgcgtgaa
     7921 gtatggacat attttatcaa ttagtgtttt ttgccgtttc ataatgtttc agattgttca
     7981 ttatgttttt gattataaac agatttgtga ttttccttgt tccagcttgt ttcacgttgt
     8041 agccctttgt gcaacgatcg gtgtagttaa tggtgtagtt aattgtgatg accggaatca
     8101 aaattatgag cagagcgctt aacaaactga gcgatacaca gctgaggaaa atcaacggca
     8161 cacccgccca aaaaacagcc tttcttaatg acggtggaaa cctgagcgtc aggcattcaa
     8221 ccagtggcct tttaacctgg tatttcactt acagggccgg aacgggaagg ggggcaccac
     8281 cggaacgcat taagctggga aattatcctg atctgagcct gaaatcagcc agggaaaaag
     8341 ccgcccagtg tcgcgcatgg ctggcagagg ggaaaaatcc acgtcatgag cttaattaca
     8401 ccgtacagga agcgttaaag ccggtaacgg ttggcgatgc gctcacctac tggcttgagt
     8461 cgtacgcaaa ggaaaaccgc gtggattatg ccgccctgaa aaagcgcctt aataatcacg
     8521 taatacagca cattggtgct atgccgctgg ataaatgcga gctacggcac tggctggcct
     8581 gttttgacca ggtggcaaag cgaacgcctg ttactgccgg attcttgcta cagacgtgca
     8641 aacaggcgct taagttctgc cggaggcggc gctatgcaat cagcaacgtt cttgatgata
     8701 tgagtgtggc ggacgttggg aaaaaaccgg atataagcga gcgtgtctta agcaccaaag
     8761 aactgggcga attattgcag gcactggaca aaaaaatatt ctccccctac tacatcgcgt
     8821 taatccgcct cctgattgtg ttcggatgcc ggacggtaga actgaggtta tcggagatca
     8881 gcgagtggga ttttaccgaa atgctctgga ccgtgccgaa agaacacagc aaaacgaagg
     8941 tggcaatatt ccggcccata ccggaagcaa tactgccgtt cgtcacgcag ctggtggagc
     9001 agaacaggca cacgggctta ttgctggggg aagtgaaaca ggaaacaagc gtgtcgcagt
     9061 acggcagatt agcgcacagg aggctaaatc accctcactg gtcactacat gacatccggc
     9121 gcacctttac aacgatgctg agcgatttag gcgtggatcc tcacgtcgtg gagcagctta
     9181 caggccacca gatgccagga atgcagcgag tttataatca ttcccgttat ctggatgcta
     9241 aacgcaatgc gctggatatg tggatggagc ggttagggat actggcggga acacatgaaa
     9301 acgtaaccgc acgaccagca gccagaagaa aataattttt ttcgtgtttt ttcagtatgc
     9361 gcatactgga taagcacaca gatacaacgg cgaacaatgt gaaacaacgt ggaacaacca
     9421 cgaacaagag gcgaaaaagt acacaggttt ataagtgact gattttattt gtgtgactgt
     9481 ttttttatac attagcgaat aacgctttaa cgcgtgagaa aaaaggcatg aaacaatatt
     9541 ttatgccttt gtattaacat gaatttaaaa gattttattc ttactgtttc attttctgta
     9601 aatcatacat cctcaatatt tgccattcat cactctagcg ggaacaatac gacacctgat
     9661 gactctttaa gaaacgaaag gggggcaata gtgttaagca ctgatcggtt tatacgtgaa
     9721 aaagaatgcg aaaaactaac cggccttagc cgtacgtgtc gctaccgcct ggaaaaggcc
     9781 ggacaattcc catcacgtcg taaacttggc ggtcgttccg ttggctggtc tttatccgag
     9841 gttctggcct ggaaggatag ctgcaaggca gttcattaat cacgctggcg gcacgcagcc
     9901 gccacacatc aatcatctga acacagagct ataaccatga agattgaata tacgccagaa
     9961 cgtgggcggg gattcgttcg ccctggtgaa actgaaaaac aacaaaattg gggtttttca
    10021 gacataaaaa aagcggcccc gaaatggagc cgcctttctg aacagataac ccgctgcgcc
    10081 gtttgtgtgt gtgatcccaa acataagaac ggcaatgata gtcgctatca ggctggtggg
    10141 caatgctatc agtctggttc agttcgttgc catacctgca atgagcgctt ttccctgtac
    10201 tctttaagga attgctcaag ggcaaaagca catggcgcga atctttctga ttcatgctct
    10261 atctttctgc gccgtctttt ccgtgccggt gataatgttt tggtcaattc tttatcggtc
    10321 attgtgttgt cctgcatagc aatgcgccgt aataccttac accacggcgc tgatggtgat
    10381 tactctggtt ctttggcctt gcgccgctgg cgatattcaa cttctcgttt taatgctgca
    10441 gtaacaaatt gccccgtact ttcgccaggc atttttaccg cctcaacatt gttcataact
    10501 tcatgcggaa cccttgccgc aacggtttgt gattttgcgt ttactgcttt tgtcgccatg
    10561 tggtgtaccc ctcataaaaa acaaatgcag tatgcaggaa aaaaaataag tgttcaacac
    10621 ttgacgtgtt taacacctgg gtttaaattg gtgttcaaca ccttgttggc gcaaggtgca
    10681 gaaacgacga aacccggcag tgcgctaaca ctaaccgggc ttctaaccac caacgatagc
    10741 gaaagtatcg aggtagctat gagaaatcat accacacacc cgcaagggcg ggactcgcac
    10801 aacctgaata aatacatctg gcgttttatc gccctgagca cggcacaacc gcgcgtgatt
    10861 accattgagg ccaccagcga acaggaagca cgccagcaat ctccggctgg ctgcgtgatg
    10921 gtattcgctg cccgtattcg tcaggaggtg tgccatgtgc aataacatcc gtccggacgc
    10981 agccgccgaa gccatcataa cgctaatgca cgcgctgatt gatatttctg ttatcgcaga
    11041 catggcgcat aagcacgcca ccagtgaaac agaatatgcc ggggctttcg ttcctcattc
    11101 gctggcggtt atgcaactta gtgcggatat ggcgctgaat gaggccaaag ccatcctgat
    11161 tgctgattgt gaaaatgggg gggttatgcg tgatgatcgt tttaattccc tgaaacagga
    11221 attttccggc gttcctgatg atgcggctga tgcgctttcg tcaatgccag aacttattat
    11281 agcggctttt ttcttacttt ccacgagaga atataaatca acggggcttg atgtactgaa
    11341 tatcgccgcc gattatgcgg aatatgtggc agaggcgcgt tatagaagaa aatttcctga
    11401 ggatgtaagc catgcgtgat atttaccacc aaacaataga ccgcgcattt cttgcacttt
    11461 ctcacagtga aaacatgctg gaaatattgc gcatatggct tgaaacactt ggcgacaatg
    11521 aacgcgacaa acaaaaatca agaattgcca cggcattaat aacgcttctt gatcctgtaa
    11581 taatggaact gcaagaaata gatctattgc acgacagata taaagaacag cacaccggag
    11641 aataaaaata atgaaactta aatattctgg cttaactgcc agtggcaaca ctcaccctaa
    11701 atttacgcgc ggtgatattt accgcgacca gtacggcggc acggtaatga ttaagggcgt
    11761 ggaagaacgg cgcgtaacct accgccgtga aggttacgaa tatgattgcg tgatgcctgt
    11821 ttatcagttc cggcgtgatt tttctctggt acagaccgcg ccgcataacg tgcccaccag
    11881 caacgccagg gcacgggcaa acatccagaa gctgaaaacc atgattaacg gattcagggg
    11941 caagaaatga aaagcgcacc gaacttaaaa aaacagcctt acgacaagat gaccgaagtc
    12001 attatttttg cgggtagtga tgcctgggca catgcgaaac agtggcagga acaggacggg
    12061 cgactggctg gcgataatgt gcctcccgtt gtgctggctg atgatcaact ggatgaactg
    12121 gcagacctga gaatcatcga cgaggggcgc tattgtgtcc ggctgtacaa ggcaggccat
    12181 atcaggccat caaatattaa tgccattgcg cacaagctgg cggcggcggg tgtaactgat
    12241 gcgaattatt accccgaagg gatgcacagc catatgcggg agaactggcg cgaatacctg
    12301 gaacgggtgc gcgggaaaga gccggcggaa gaaaaaaacc accagcgaaa aaccacgcta
    12361 ccgatgagcg ttggatctac cggatacgac acgcaactgg attacgtggt taaggggatt
    12421 attccggcgg tatcgctatg cagcatatac ggggctagcg ggtcctataa atcattcctt
    12481 gccggatcgt gggcgtgcca tgttgccact ggtcgccagt ggggaggccg cagggttgca
    12541 catggtgcgg ttctctatgt ggttggtgaa ggcggtatag gtgttccgcg tcgtgtaaaa
    12601 gcctgggagg ttgtgcacga tgagcaggtg aaaaatctgt atctggtaaa ccgccccatc
    12661 tttccggctg ccccgcttga tgttgatgaa atggttatcg ctgcccgtca ggtggagcgg
    12721 gaaacgggta aacctgtacg catgattatt ctggatacgc tggcgcgttg ctttggtggg
    12781 aatgatgaaa atgattcccg tgatatgggg gcgtttatcc gtggttgtga cgaactgaaa
    12841 cgacgcacag gggccacggt gctggtggtt caccattccg gcaaggatga gacgaaaggc
    12901 gcgcgtggtt ccagtgcatt tcgtgcttcg ctggatgctg aataccggat acgcagggag
    12961 gacgcaggaa gcgaagcgct ggttatctca tgcaccaaaa tgaaggacgc ggaggaactc
    13021 aaagaagccg catatgactt acgcgtggtg gagcttttta ccgacgctga cggtgaatta
    13081 atcacgtcgc tggtggtggt ggatgatccg cgccctcctg ttgaactgga gcgcatcgag
    13141 gaggcaggga acaagacgga aaaccatacc gcgctatggg ggtgcatccg ttcacgcaca
    13201 cagaacggcg acaagtgcac gatcccgctg ttacgtgatg acatgaaaaa gctggggtat
    13261 gaaatgaaaa acttccggcg ctggctgtac aagctggaaa aagatggggt tattcgtatc
    13321 gatggggatg atgtagcgcc gctataaaag tgaggagcaa aagcgagggg gatagaaaga
    13381 gggccaaaat tagcccgctc tccctcactt ttcgacctgt atacatcctc aaaagtgagg
    13441 ggtaaaaaaa tacttatgaa acacacacat agaaaaaccg aaaatcccaa ctgcgacgaa
    13501 gtgagacgct tgaaaaagtg aggcgaaaaa gtgagaggtt gcgagaaatg acccaaaaac
    13561 gcagagacag aacagagcca aaatataaag cgttagacat gactgagcac accttaaagg
    13621 tggcaatcag aacgatagac cgccacacgc gggaaggata cgcgaaggaa catcccgacc
    13681 tgataagcgc attcatgacc acggcggcgg caaactttgc cacgctgaca gaacgggaga
    13741 ttgccgaagc ggaacaggta acaaccatca acgttaaaac cggagaggtg gaattatgac
    13801 ggcacagata gccgcttacg ggcggctggt ggacgacccg caggtaaaac agaccagcaa
    13861 gggcacaccg atgacgctgg cgcgtatggc ggtatctttg ccatgcagcc aggcacagga
    13921 cggacaggcg acgttatggt tatcggtcat cgcatttggc aaacaggccg acttcctggc
    13981 taaacatcaa aaaggcgatg ttgccagcgt atccggcacg atgcaggtaa gccagtggac
    14041 cggacagaac ggagaaacgc ggcagggctg gcaggttatc gcagacagcg taatcagtgc
    14101 ccgcgcggca cgtccaggcg ggaacagacg caaaaccaca ggcacacagg gtaatcagcc
    14161 accagcggga ggcgatgacc cttacggtga tgatatttcg ttctgagggg gtgacgatgg
    14221 tacatgaccg catagcggag gaactggagg cgaaaggctt ttaccggagg gcggcggcgc
    14281 gatggggtga agtcatgcag ctggtggaga cagacaagga acggcatcac atcacgatgc
    14341 gacggctgga atgttcaagg aaggcacaga gggcaccgga gccgccggat aatttcggag
    14401 acctgaaaaa ggcagtcgat cgcacttatg ccgaaatggg tatagatggt gctggtgatg
    14461 aaatatggcg caattaccag gacagctaat caaacagccg gagaaatccg gctttttttg
    14521 caccagttga aacggtatgg cgcattaccg ggttttcgtc acggtcaggc atagttacta
    14581 tctgaaacaa acagacacaa cagaggaaaa aaacaatgcc gatgaaattt gatgagatat
    14641 taaaacagcg tgataaatac catgctgaca acatggagac gatgagcatc aatgattacc
    14701 gcgcattcct ggagacgggc gcactgattg aaaaggatca gcatggtttt gtgagatgtg
    14761 ctctatccgg tgaaatgctg gcggtaaatc ctgaacagat agatgcattg atagaatttc
    14821 tgaaagagat cagagactga gccagcacac agcacacata gccggagcaa tccggctttt
    14881 ttgcgcccaa aaaaagcccg ataaggtcag agggttctta tcgggctttt gcatatgagg
    14941 tttttggatg cactggcgtt cgtgatcggg ataatcattt cataatttgc aacataactc
    15001 aatatcattg cataaaatgc aattctgatt ataatcagga ctggataaac atccagttat
    15061 gattttttaa gtcgaagagg aatttcttac tatggctgaa gagaaaaaag gcggtgtttc
    15121 ggtgtacata agccccgaca tcgtgaaggc gctcaaggaa cgccaccagc agaacgtaaa
    15181 agcaggcatt gcggcaggac ttgatccgct ggcgatggtt gagccgtcaa caggctggca
    15241 ggtccgcgcc tatttacgcg cggcgctggg catgaatcag acgcatggag gtgaataatg
    15301 gcaggcaaag caacggcact taacactaac cagcttttcg cgtacctgaa tcgcggggat
    15361 attgcagaat ttaaattcag tccgctgttt accacgctgt ttttcccgaa cgtggcgacc
    15421 ttcaacaccc agaacatcat gctggacacc ctggacattg aagaggttac gatgtcagcg
    15481 ttctgttcgc ctatggtggg tagtcaggtc cagcgcgata aagggtacga aaccagcacg
    15541 attaaaccag gctacatgaa acccaaacac gaaatcgatc caacaaaaac catcatgcgc
    15601 atggctggag aagatccggc acagcttaac gatcctacct atcgccgtat gcgcctgatt
    15661 actggcaaca tgcgccgcca gataaacgcc attaaggcac gcgtggaatg gctggcggtg
    15721 aatgcggtaa cgaccggaaa aaacctcatt gagggcgaag gcatagaacg ctatgaaatc
    15781 gactggaaga tcccggaaag caatatcata gaacaggctg acggtaaaaa atggtctgag
    15841 ctggataaag aaacacacga tccaatctat gacatcgagc tttatgctga tcagggcaat
    15901 tgccccgcaa acgtcatgat tatgggcgct gaagtatggc gcacgttacg cagctttaaa
    15961 aaattccgcg aactgtacga cctttcccgt ggttcagaat ccgccgcaga actggcctgt
    16021 aaaaacctgg gtgaagtggt gagctttaaa ggctatctgg gcgatattgc ccttgtcgtc
    16081 tattccggca aatacactga cagcgacggc accgaaaaat atttccttga gcctgatttg
    16141 ctggtcctgg gtaacaccaa caataaaggg ctggtggcct atggtgcgat tatggatcag
    16201 gaagcggtca gaacgggcgc aacgcaaaac atgttttacc cgaaaaactg gattgaggac
    16261 ggcgatccgg cgattgagta cgtgcagacg cacagcgcac cgcagccggt accggcagat
    16321 attcgcaaat ttgttaccgt caaaattggt taacgggggg attctatgga cactccatac
    16381 attgagttat ttgcaggtag tcagcaggta tccacgacgc tggtacattt tgctgctgat
    16441 gctggcgtta ttcaggaatt taccccgctg atgctggcgg aaaatggcga gtttaagccg
    16501 tgggatggtc aggaatctgg caaggctgtt tatctgacct cgtaccccgt ggacacgtcg
    16561 acgcagaaat cagcacagtg ctacaagacg gggatattca atattgccgc cgttaactgg
    16621 cctgagagcg ccgacaccga cgcgaaaaaa tgcgccgcct ttgcgggttc tggcgtatcc
    16681 gttcagccgc tggcgcgata agcaggggga acgatggcaa cgaatgaaag tatcatgacg
    16741 ctaccgctgg cgagtaaatt taaagccgaa gcgcgggcaa tggctgacag aggtttatca
    16801 acctacgagg ccgtatatca acttaacagc ctggaagaga aggacaagcc gcgcgctgat
    16861 gcgattatgg cgcttcatga atctgacgac tatcagccgc tgttacgtgc aatggcaaac
    16921 gtgccttgta ttgatgttga tacggctaaa aacatcctga acatgaccat agagcaggaa
    16981 cgcccgaagg ttgcaccaga gcttaccgca gcctttgaaa actttatgga catgcacagc
    17041 ccacaagccg tatcagctgg catggcatac gatggcagaa acccgggcga tgacggcgac
    17101 atcgatcgca tactgaaaac catctgagac aaggccggag aaatccggct tttttgcggg
    17161 tcctttccgg catatggacc tgttacgggg cggagacctc gcggattttc gctatttatg
    17221 agccttttca ggggggtggt ggtggttttg ttgtttgatc tatctttatg aatgaaaagg
    17281 gaaagatgca agcaatacac caacctgaag cagtaattaa gttggtgtat taatgaaatc
    17341 tcatctgatg aacaaaaaaa acatggcgaa aagctgccgt gtaagtgcga cagcgttcga
    17401 caagtgggga gtaactcccg ttgagcgtaa aggccgtgag gcgttttatg atgttgccag
    17461 cgtgatagaa aatagggtta acaatgcaat tagccagctt ataaacgaca aaggcgagat
    17521 tgacgatgat gaacttttac gagtcaggat cagattactg acagcacagg cagaggcgca
    17581 ggaacttaaa aacgatcgcg atcgcggtga cgtgattgat actgagtttt gcctgtacgc
    17641 gctttcaaag ctggcgagtc agatttcatc aatcatggac atcctcccgc ttactatgca
    17701 aaggagcttc ccacagatta cccccgccat gctggacagg cttaaaaggg aagtggttaa
    17761 agtctgtaat gccagtgcca gagttgccga caacctccca cagatactgg ctgattactt
    17821 gaaagaaaca accggaaacg taccggaaaa gttgctacag aagaaaggcg agtaacagac
    17881 gcgcaattat tgaacaaaaa tgagaaagga tctgataatg gcattttctg gctaaaatgc
    17941 ctcgtgtcag atcctttctt tttttaatat tttcattgtt aaacagtctg ttacagagaa
    18001 gaagatcgga tctgactttt gccggaaaat tctcataaac agataaaaac cgcgaggtcg
    18061 ccgccccgta acgggccata attccaggaa ggacccgacg acaccagact atcagagcga
    18121 tgggggcaca atgacagaag ccgaactact gggattaatc cgccgcgtcg ccggaatcag
    18181 ccagcagcat gacgaacagg ccacacagcc ggacagcgtg accgctgaaa attatgtgcg
    18241 tgttgtagcg gaagtgatgc gccgtgatgg tatagaactt aatggcgttg atatgcgcaa
    18301 catacggaac cgcgttcttg aaatgctggc ctacaatcgc cgcgttgaac tgtatcggga
    18361 gaaagaaaaa ataacgtacc actggaagaa gccggaatgg ctgcggcggt aacttgctga
    18421 tattcccgat aacgcaaaat tgcgctggct acatccctca catctgagga ctgttaacca
    18481 gaaggccgga atatattcac aaactacgaa attttcgtag tttaattaat ggtcagatta
    18541 atcgctaacc cgctgatatt ttcggaaacc tcaatttgag gaagtcggaa gcgtcaccct
    18601 tcatattctg aatggtcata atgaccacgc agaccatcaa ggcaaccaca acgaacatcg
    18661 ttacggttgg agcttttacc ccactggggg aaaggtatta cgataatcat aacacctacc
    18721 gaacaggtaa aacccaccag cctgattaac agttaaccgg aaaaaaaatc cggtagcccc
    18781 gttatgggga tccccatatc gacattaacg cccctcatga attggtgcgc ttccccctgg
    18841 aaagattacc cgcctgtata tttcttgtgt ctatttgttc cacgttgttt catacagtgc
    18901 accgaacggt gtagttaatg gtgtagtcaa tttgctgttt ttagcacttt ttgcagtgtg
    18961 tgttgtttaa ttaaatcaat gggttgcctt gctgtggtga taaaaggaca tatttattca
    19021 tctttcggcg cagaatgctg gcgaccaaaa atcacctcca tccgcgcacc gcccagcatg
    19081 ctctctccgg cgacgatttt accctcatat tgctcggtga tttcgcgggc taccgccagc
    19141 cctacacctt gcccagggcg taaagtatca acccgttgac cacggtcgaa aatgacctct
    19201 cgcttgctta atggaatacc ggggccatca tcctcgacca caatatagag atgctcgtcg
    19261 gtttgccttg cagaaatttc gacaaactcg aggcaatatt tacaggcatt atccagcacg
    19321 ttgcccatca cctcgacaaa atcgttctgc tcaccgacaa agctgatctc tggcgaaata
    19381 tcgagagaga tattgacccc tttgcgttga tacactttgt tcagcgctga ggtgagattg
    19441 tccagcagtg gggcgaccgg atgcagctcg cggctgagca atgtcccgcc gcgcatactg
    19501 gcacgatgca ggtagtagcc aatttgctgt gaaatgcggc tgatttgctc cagcattacc
    19561 ggctcagcat cactgacgct catcttttca ctacgcagag aacgcagcgt actttgcagc
    19621 accgccagtg gcgttttcag actatgggtc aggtcggtga gcgtcgtacg gtatttgtcg
    19681 taacgttcgc gttcactttt taacaatcgg ttcaggtttc gtaccagact ggtcagttct
    19741 cgcgttgtgg ctggattgag caattcgcgg ttatgttctt ccagttcgcg gacttctttt
    19801 gccagggctt cgatggggcg taaactccac caggcggcga cccacagcag cgggatcact
    19861 aacagcagat tggctgagag cacatagata aaccagctcc agaccatata ggaacttttt
    19921 agctccaccg gaatggtatc caccaccaca atggttaatt ttggcatccg cgatgttgcc
    19981 gggtagacgt ttactgccac cgagtgggtc atctccgcgt cgtcatcatc ttcccgcact
    20041 tcctgcaact gttgctgtat cgaatgatct ccactcagca agaggctggt atcgttaaca
    20101 tccgcttcaa tttcatgaaa accattcgat ttcagccagt caggctggat catcttcatc
    20161 agccagggca cgtcacgttg cgcccataaa agctgcccgt tctcatcata aattagcgtc
    20221 atggtggggc tttgcttgtc gatattttcg ggtaactcga catgcaactt attgttttcc
    20281 cacttcgcaa gggtatagaa cagattgctc tcgccacgta acagccgaaa cgtagtttta
    20341 tcgaaactga cgctataacc gatcagcgcg accattccgt aggcaagcga aagcaccagt
    20401 actaccgcag ccgttgccaa cagaaaacgt acccgcagtg agagcgggaa aaaaagacgc
    20461 agtaattttt tcatcagcgc aattcgaaaa aatagccctg gccgcgaacg gtagtaatca
    20521 cttcttgcgg atattgtgcc tgaatttttt tgcgcagacg tcccatcagt acatcaatgg
    20581 tatggctttc ccgcagctcc gcatccggat agagttggag cattaacgaa tctttgctca
    20641 ccactttgcc attattgcgt atcaacgttt ccataatggt gtattcgaac gcggtcagtt
    20701 tgatcacttc gtcattaata gataattcac ggcgagagag atcaacctga aacgggggta
    20761 gcgaaatgac ctgtgaagcc aggccgctat tacgccgcat taatgcctgc attcgcgcca
    20821 tcacctcttc aatatgaaac ggtttagtca cgtaatcatc agcaccggca cttaatactt
    20881 cgactttgtc ctgccagctt tcacgggcgg ttaataccag aatcggcagt gaaacatcat
    20941 tgctacgcca gcggcgaatc agtgacagac cgtcctcgtc aggcaatccg agatcgacaa
    21001 tcgcaatatc cggtaaatgt tcattgagat aataatcggc ttctttggca tcttctgcgt
    21061 catcgacctg atgaccagca tcctgaatct gaactttaag gtggtgacgt aacaacgcat
    21121 tgtcttcaac aaccagtacg cgcattttta tttctccctg tcttaattat taaaatagtg
    21181 taacgcgatt atgtgtttat gggggtaaac attaaataaa ccagcgggga ggggaggtaa
    21241 agtgaaaaaa gaaaaagtgg ataattttaa taaacaggcc ggacagcatc gccatccggc
    21301 actgatacga gtttatttca gctcatcaac catggtgata gctcgaccaa tatagttagc
    21361 cggcgtcatc gctttcaggc gggctttctc ttcttctggc aacgccagac catcgataaa
    21421 ctgcttcatg ccttcggcgt caacgcgctt accgcgagtc agctctttca gcttctcgta
    21481 cggtttttcg atgccatagc gacgcataac tgtctggatt ggctcagcca gcacttccca
    21541 gttgtgatcc agctcatcca gcagatggtc acggtttact tccagtttgc tcacgccttt
    21601 cagggtggat tgatacgcaa tcagcgcata accgataccc acgccgaggt tacgcagcac
    21661 ggtggagtcg gtcaggtcac gctgccagcg ggaaaccggc agtttgcttg ccagatgctg
    21721 caataccgcg ttggaaaggc ccagattccc ttcggagttt tcgaagtcaa tcgggttaac
    21781 tttatgcggc atggtggaag aaccaatctc accagcaatg gttttctgtt tgaagtggtt
    21841 aagggcgata taaccccaga cgtcacggtc aaagtcgatc agaatggtgt tgaagcgcgc
    21901 aacgcaatca aacaattcgg caatgtagtc gtgcggttcg atctgggtgg tgtacgggtt
    21961 ccactgaata cccagcgagg tgacgaactc ttcgctgaac tgatgccagt caacttccgg
    22021 gtaagcggcg atatgggcgt tatagttacc gaccgcgccg ttgattttgc cgaggatctc
    22081 cacctgatta agctggcggt actggcgctc catacggtag gcgacgtttg ccatctcttt
    22141 accgatggtt gacggcgtgg ctggctgacc gtgggtacga gacagcagcg ggatatcgcg
    22201 gtactgaacg gcgagatctt taatgccatc aatcagttga cgccagtacg gcaggatcac
    22261 ttcatcacgc gcggttttca gcattaatgc gtgggagagg ttattgatat cttccgaagt
    22321 acaggcaaag tggatgaatt cagaaaccgc gtgcagttcc gggatctccg ccactttttc
    22381 tttcaggaaa tactcaaccg ctttaacgtc gtggttagtg gtacgctcga tggttttgat
    22441 gcgtgcggca tcttcttcgc tgaaattggc gacaattgca tcaaggtaac cgattgcgtc
    22501 ggcagcaaaa gcaggaactt ccttgatcgc tgcgtgcgcg gccagttttt gcagccaacg
    22561 tacttcaact tgtacacgga atttcagcaa accatattcg ctgaaaatcc cgcgcagcgc
    22621 gctgacttta tcgccgtagc gtccatctac aggggaaacg gcggtcagtg aggataattc
    22681 catagatcac aactccgggg ttaaatgagc aagaatttgt tttgcctgag tggtcaggcg
    22741 attacgagaa aacatcagtt gcagacgtcc gccgccgacc tggtgccaga gcacggcggc
    22801 gcgaatgcct gccagcaggg ttgcgcgaac tttcgcctgc acttgtgggc tttgcagtac
    22861 agcaggggaa ccggtgacct gaatgcgcgg gccaagcggg ctgatcacat caacatagat
    22921 agcagccatc gcgctcatca gcgtttcgga ctgtaaatcg aagtgttcga gctggcgttg
    22981 caggccgttg atccggttgc ccagagtgtc gagcgcgcct ttcgctgagg agagtttgcg
    23041 ctcaagcacc atcaagctga gtgtgtagcg ggttaattcg gcgtttaagc cctggcgact
    23101 gctggcattg agcacgccga gcagggtttc cagcccgacg cgcaggttgg cttcgctacc
    23161 gccaaaaacc gccagcgtcg agctggggtt catatcaata atactgttga gtgagacgtg
    23221 tagcgcatcg gcatcacaat gcccctggtg agcgagttgt tgcaccaggc gtgccgactg
    23281 acaaataccg gccagggcga gggtgatgtc atagtaattc tttgccacgt tcactgcttc
    23341 cttgttttaa gtaaagataa taatcagacc ggcagcggca gacgctgctc aataataccg
    23401 ccaccgaggc acacttcacc gttatagaag acggcagact ggcccggcgt cacggcggca
    23461 accggttcat cgaaaatcac ttcaatgcga tcatcgtcca gcgccttgac ggtgcaaggg
    23521 atatcggtct ggcgatagcg ggtttttacc gtgcaacgca tagtgccggt gaatggttcg
    23581 cgatcgaccc agtgcaactg ctgggcaatc aacccgacag acatcagccg cgggtgttca
    23641 tggccctgag cgacaaccag aatgttgttt tcgacgtctt tgtccaccac ataccacggt
    23701 tcttcggtac cttctttggt gccaccgata cccagacctt tacgctgacc gagagtgtga
    23761 tacatcagcc cctggtgctc accaatttca tcgccatcga cggtaatgat tttgcccggt
    23821 tgcgccggga gataacggcc caagaactcg cggaatttac gttcgccaat gaagcaaatg
    23881 ccggtagagt cttttttctt cgcggtgacc agaccaagat cttcagcaat cttacgcacc
    23941 tgcggttttt ccagttcgcc gaccgggaac aggctttgcg caatctgctc atggctgagc
    24001 gtataaagga agtagctctg gtctttattg ctgtccagac cacgcagcag gcggctcttg
    24061 ccatcgacat cggcccgacg tacgtaatga ccggtagcga tataatcggc acctaaatct
    24121 tcggcggcaa attcgaggaa ggctttaaat ttgatctctt tgttgcacag aatatccgga
    24181 ttcggcgtgc gaccggcttt atattcggca aggaacagtt cgaagacgtt gtcccagtac
    24241 tcggcagcaa agttaacggt gtgcagttca atgccgagct tgtcgcagac agcctgggca
    24301 tcagccagat ccgctgccgc tgtgcaatat tcctcaccgt cgtcttcttc ccagttcttc
    24361 ataaacaggc cttcgacctg atatccctgt tgttgcaaca gccaggcaga aacggaggaa
    24421 tcgacaccgc cggacatgcc gacgattact ttttttgcgg tttcagacat tggatcactc
    24481 acgacattga acttcaaggc ggcgtattct atcacgcagc cttttagatg acaccctttg
    24541 taaaaggcca gttaaaatcg ccaatcatct ccagcggata acgttgcccg ctttgataac
    24601 aacgaatact ttccgccacc agcggcgaac gaagatttga cgcctgtaaa atttcttcgg
    24661 cgctgaccca acggcagcag tcgatatcgc tgtcatgagg ctgggtcggg catatttgct
    24721 caagctcaat ggcaaagagg aaacgcaaaa acggcgtttt atctggcgca atccactgat
    24781 gcatacgaat aaagtgttgc ggttgcgcgc tgatgccggt ttcttcccat agctcacggg
    24841 cggcagcttc cactaaggtt tcatcggctt ccagatgccc ggcaggttgg ttccataacg
    24901 ccttaccatt aatcgtctct tcaacgacta aaaatttacc ctctgcgtgc actacgcaag
    24961 caacggtaac gtgcggttta aacattgtgc atccttaatc tgtcacttct cgccattcgc
    25021 cattggcaag attatccaaa gagtaatctc ccatcgcata gcgaatcagt cgcagcgtgg
    25081 ggaaaccaac atgggcggtc atgcggcgca cctggcgatt acgtccttca tataaggtga
    25141 tcttcagcca gctggtggga atacttttgc gttcacgaat tggtggattc cgcggccata
    25201 accacgctgg ttcgtcaacc agctccgcgc cggcgggcag ggtagggcca tcatttaagg
    25261 ttacgccatt gcgcaaggct tcaagtgcgt cttgtgtggg aataccttcc acctgcacat
    25321 aatagatttt cccggtgcgt ttgcccggct gggttaaacg cgcctgcaac gcgccgttat
    25381 tggtcagcac cagtaaccct tcgctatcgc ggtcaagacg acctgctgca taaacgccct
    25441 gaaccgggat gaattctttt aatgttttgc gtccggcttc atcggtgaac tgcggaagaa
    25501 catcgtaggg tttattgaac aggatcacac gcgtgggctg gttttcaggt ttacgcctgg
    25561 tagaacgttg cgagctgaat cgcttaacct ggtgatttct aaaagaagtt ttttgcatgg
    25621 tattttcaga gattatgaat tgccgcatta tagcctaata atgcgcatct ttcatgacgg
    25681 caaacaatag ggtagtattg acaagccaat tacaaatcat taacaaaaaa ttgctctaaa
    25741 gcatccgtat cgcaggacgc aaacgcatat gcaacgtggt ggcagacgag caaaccagta
    25801 gcgctcgaag gagaggtgaa tggaaagtaa agtagttgtt ccggcacaag gcaagaagat
    25861 caccctgcaa aacggcaaac tcaacgttcc tgaaaatccg attatccctt acattgaagg
    25921 tgatggaatc ggtgtagatg taaccccagc catgctgaaa gtggtcgacg ctgcagtcga
    25981 gaaagcctat aaaggcgagc gtaaaatctc ctggatggaa atttacaccg gtgaaaaatc
    26041 cacacaggtt tatggtcagg atgtctggct gcctgctgaa acccttgatc tgattcgtga
    26101 atatcgcgtt gccattaaag gtccgctgac cactcctgtt ggtggcggta ttcgctctct
    26161 gaacgttgcc ctgcgccagg aactggatct ctacatctgc ctgcgtccgg tacgttacta
    26221 tcaaggcact ccaagcccgg ttaaacaccc tgaactgacc gatatggtta tcttccgtga
    26281 aaactcggaa gacatttatg cgggtatcga atggaaagca gactctgccg acgccgagaa
    26341 agtgattaaa ttcctgcgtg aagagatggg cgtgaagaaa attcgcttcc cggaacattg
    26401 cggtatcggt atcaagccgt gttctgaaga aggcaccaaa cgtctggtcc gtgccgcgat
    26461 tgaatacgca attgccaacg accgtgattc tttgaccctg gtgcacaaag gtaacatcat
    26521 gaagttcacc gaaggcgcgt ttaaagactg gggctaccag ctggcgcgtg aagagtttgg
    26581 cggtgaactg atcgacggtg gcccgtggct gaaagttaaa aacccgaaca ctggcaaaga
    26641 gatcgtcatt aaagacgtga tcgctgatgc attcctgcaa cagatcctgc tgcgtccggc
    26701 tgaatatgat gttatcgcct gtatgaacct gaacggtgac tacatttctg acgccctggc
    26761 agcgcaggtt ggcggtatcg gtatcgcccc tggtgcaaac atcggtgacg aatgcgccct
    26821 gtttgaagcc acccacggta ctgcgccgaa atacgccggt caggacaaag taaaccctgg
    26881 ctctattatt ctctccgctg agatgatgtt acgccatatg ggctggactg aagccgcaga
    26941 cctgattgtt aaaggtatgg aaggcgcaat caatgcgaag accgtaacct atgacttcga
    27001 gcgtctgatg gaaggcgcta aactgctgaa atgttcagaa tttggtgatg cgatcatcaa
    27061 gaacatgtaa tcactacatg tgtttaatat tgcaacgggc gtataacacg cccgttgttt
    27121 tatttatgta ggtattatta atagcatatc gagcatattt atataaaacc ctttacttga
    27181 gcccatatgg gcatattttt ataatgcaac tattatgcaa acatttattt gttatttttc
    27241 tttctcctgg aggacactct tgactgcttt tgagtagact ccataaatcc ttgttgaatg
    27301 gtgcgatgtt ataaatagta ataggatatt ctttatcttt aaggataatt ccagatttaa
    27361 ccggtgtaaa tatactgcca ggagggagaa atatagtaga ttgataccag atgatcattt
    27421 tcatattacc ccatatggct gaaaaagata tgccgcatga aggttgaatt atcgtgtcaa
    27481 ttactatcca cttcatttgt tatgtcttat cccacggtat ttaatatagt tcatttggat
//
LOCUS       DAZKIV010000050        27083 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZKIV010000050 DAZKIV010000000
VERSION     DAZKIV010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.050"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1441)
                     /locus_tag="ACHOT6_002103"
     CDS             complement(<1..1441)
                     /locus_tag="ACHOT6_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFP9947323.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGITFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMVIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQRGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVM"
     gene            1820..3442
                     /gene="pckA"
                     /locus_tag="ACHOT6_002104"
     CDS             1820..3442
                     /gene="pckA"
                     /locus_tag="ACHOT6_002104"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFP9947324.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(3518..4870)
                     /gene="envZ"
                     /locus_tag="ACHOT6_002105"
     CDS             complement(3518..4870)
                     /gene="envZ"
                     /locus_tag="ACHOT6_002105"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFP9947325.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLSVPVTRAQGTTKEG
                     "
     gene            complement(4867..5586)
                     /gene="ompR"
                     /locus_tag="ACHOT6_002106"
     CDS             complement(4867..5586)
                     /gene="ompR"
                     /locus_tag="ACHOT6_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFP9947326.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            5814..6290
                     /gene="greB"
                     /locus_tag="ACHOT6_002107"
     CDS             5814..6290
                     /gene="greB"
                     /locus_tag="ACHOT6_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFP9947327.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            6387..8708
                     /locus_tag="ACHOT6_002108"
     CDS             6387..8708
                     /locus_tag="ACHOT6_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFP9947328.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            9146..9373
                     /gene="feoA"
                     /locus_tag="ACHOT6_002109"
     CDS             9146..9373
                     /gene="feoA"
                     /locus_tag="ACHOT6_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFP9947329.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            9390..11711
                     /gene="feoB"
                     /locus_tag="ACHOT6_002110"
     CDS             9390..11711
                     /gene="feoB"
                     /locus_tag="ACHOT6_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFP9947330.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            11711..11947
                     /gene="feoC"
                     /locus_tag="ACHOT6_002111"
     CDS             11711..11947
                     /gene="feoC"
                     /locus_tag="ACHOT6_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFP9947331.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            12150..13028
                     /gene="rpnA"
                     /locus_tag="ACHOT6_002112"
     CDS             12150..13028
                     /gene="rpnA"
                     /locus_tag="ACHOT6_002112"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFP9947332.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(13057..13827)
                     /gene="bioH"
                     /locus_tag="ACHOT6_002113"
     CDS             complement(13057..13827)
                     /gene="bioH"
                     /locus_tag="ACHOT6_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFP9947333.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIVLTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDVRA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            13865..14548
                     /gene="gntX"
                     /locus_tag="ACHOT6_002114"
     CDS             13865..14548
                     /gene="gntX"
                     /locus_tag="ACHOT6_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFP9947334.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            14607..15182
                     /gene="nfuA"
                     /locus_tag="ACHOT6_002115"
     CDS             14607..15182
                     /gene="nfuA"
                     /locus_tag="ACHOT6_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFP9947335.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            15543..16859
                     /gene="gntT"
                     /locus_tag="ACHOT6_002116"
     CDS             15543..16859
                     /gene="gntT"
                     /locus_tag="ACHOT6_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9947336.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(16904..18988)
                     /gene="malQ"
                     /locus_tag="ACHOT6_002117"
     CDS             complement(16904..18988)
                     /gene="malQ"
                     /locus_tag="ACHOT6_002117"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFP9947337.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(18998..21391)
                     /gene="malP"
                     /locus_tag="ACHOT6_002118"
     CDS             complement(18998..21391)
                     /gene="malP"
                     /locus_tag="ACHOT6_002118"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFP9947338.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            22003..24708
                     /gene="malT"
                     /locus_tag="ACHOT6_002119"
     CDS             22003..24708
                     /gene="malT"
                     /locus_tag="ACHOT6_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFP9947339.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(24751..25767)
                     /gene="rtcA"
                     /locus_tag="ACHOT6_002120"
     CDS             complement(24751..25767)
                     /gene="rtcA"
                     /locus_tag="ACHOT6_002120"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFP9947340.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(25771..26997)
                     /gene="rtcB"
                     /locus_tag="ACHOT6_002121"
     CDS             complement(25771..26997)
                     /gene="rtcB"
                     /locus_tag="ACHOT6_002121"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFP9947341.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAALGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
BASE COUNT         6434 a         7449 c         6856 g         6344 t
ORIGIN      
        1 tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag
       61 aaccaggggc aattaacaca ttttcggcta atactttttc cgatacctgt tcctgaagat
      121 gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct ttcataaacc
      181 cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgacctctc tgttcgagca
      241 tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc atatgactgt
      301 tcacgctaat gcgcatactg tccagcaccg gcgaattaag aagcaccagt atcaccaaca
      361 ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt tgcccattac
      421 gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc accgccagcg
      481 cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat aacgcccagg
      541 cggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg caacgcaacg
      601 gccccgtcca gggaagtgac gctttttgcg gatcgcggac aataaccatt aagatcaatt
      661 gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa atcgcgctca
      721 ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact aacggcagca
      781 accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt gactgtgtac
      841 gcgcgaggat caccgccagt gcggtaacca gacctaatgt aagatacata aaccagtcgg
      901 ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac tcaccccaca
      961 gtaaaagcac cagccacgtc aggccattgg cgagaaaaat caccagtaat ataagtacgt
     1021 tatgccatac tgactggtaa aaatagcgat agcggctact gtcattgcgg atgcgaaggc
     1081 tttgttgtat ccacggtagc aacaacattg ccatcagcag cagatagcaa ccaaaatccc
     1141 aaagcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc aaccagccgc
     1201 tcatccccag cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa
     1261 aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc acgccataaa
     1321 caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa accagcccct
     1381 gaagtaatcc ggtagcaatc atcccccagc gtgttgccgg tgaaagttca acgttatcca
     1441 tgttggttat ccagaatcaa aaggtgggtt aattatcgca tccgggcagt agtattttgc
     1501 ttttttcaga aaataatcaa aaaaagttag cgtggtgaat cgatacttta ccggttgaat
     1561 ttgcatcaat ttcattcagg aatgcgattc cactcacaat attcccgcca tataaaccag
     1621 gatttaacct tttgagaaca ttttccacac ctaaaatgct atttctgcga taatagcaac
     1681 cgtttcgtga caggaatcac ggagtttttt gtcaaatatg aatttctcca gatacgtaaa
     1741 tctatgagcc ttgtcgcggt taacaccccc aaaaagactt tactattcag gcaatacata
     1801 ttggctaagg agcagtgaaa tgcgcgttaa caatggtttg accccgcaag aactcgaggc
     1861 ttatggtatc agtgacgtac atgatatcgt ttacaaccca agctacgacc tgctgtatca
     1921 ggaagagctc gatccgagcc tgacaggtta tgagcgcggg gtgttaacta atctgggtgc
     1981 cgttgccgtc gataccggga tcttcaccgg tcgttcacca aaagataagt atatcgtccg
     2041 tgacgatacc actcgcgata ctttctggtg ggcagacaaa ggcaaaggta agaacgacaa
     2101 caaacctctc tctccggaaa cctggcagca tctgaaaggc ctggtgacca ggcagctttc
     2161 cggcaaacgt ctgttcgttg tcgacgcttt ctgtggtgcg aacccggata ctcgtctttc
     2221 cgtccgtttc atcaccgaag tggcctggca ggcgcatttt gtcaaaaaca tgtttattcg
     2281 cccgagcgat gaagagctgg caggtttcaa accagacttt atcgttatga acggcgcgaa
     2341 gtgcactaac ccgcagtgga aagaacaggg tctgaactcc gaaaacttcg tggcgtttaa
     2401 cctgaccgag cgcatgcagc tgattggcgg cacctggtac ggcggcgaaa tgaagaaagg
     2461 gatgttctcg atgatgaact acctgctgcc gctgaaaggt atcgcttcta tgcactgctc
     2521 cgccaacgtt ggcgagaaag gcgatgttgc ggtgttcttc ggcctttccg gcaccggtaa
     2581 aaccaccctt tccaccgacc cgaaacgccg cctgattggc gatgacgaac acggctggga
     2641 cgatgacggc gtgtttaatt tcgaaggcgg ctgctacgca aaaactatca agctgtcgaa
     2701 agaagcggaa cctgaaatct acaacgctat ccgtcgcgat gcgctgctgg aaaacgtcac
     2761 cgtgcgtgaa gatggcacta tcgactttga tgatggttca aaaaccgaga acacccgcgt
     2821 ttcttatccg atctatcaca tcgataacat tgttaagccg gtttccaaag cgggccacgc
     2881 gactaaggtt atcttcctga ctgctgatgc tttcggcgtg ttgccgccgg tttctcgcct
     2941 gactgccgat caaacccagt atcacttcct ctctggcttc accgccaaac tggccggtac
     3001 tgagcgtggc atcaccgaac cgacgccaac cttctccgct tgcttcggcg cggcgttcct
     3061 gtcgctgcac ccgactcagt acgcagaagt gctggtgaaa cgtatgcagg cggcgggcgc
     3121 gcaggcttat ctggttaaca ctggctggaa cggcactggc aaacgtatct cgattaaaga
     3181 tacccgcgcc attatcgacg ccatcctcaa cggttcgctg gataatgcag aaaccttcac
     3241 tctgccgatg tttaacctgg cgatcccaac cgaactgccg ggcgtagaca cgaagattct
     3301 cgatccgcgt aacacctacg cttctccgga acagtggcag gaaaaagccg aaaccctggc
     3361 gaaactgttt atcgacaact tcgataaata caccgacacc cctgcgggtg ccgcgctggt
     3421 agcggctggt ccgaaactgt aatgatttga agctggagaa tatctatcca gtatcttata
     3481 gaaagcaaaa cgggaggcac cttcgcctcc cgtttattta cccttctttt gtcgtgccct
     3541 gcgcccgcgt taccggcact gacagccagg cgcgaatgga aagcccgccc cgctcgctgg
     3601 tgccaagctc cagcatcccg ttatggttat ccacgatacg ctgcacaatt gccagcccta
     3661 atcccgtgcc gctaatggtg cgcgcactgt cgccgcggac aaacggctgg aacaggtgct
     3721 tacgttgttc cggcgcaatt cccggaccgt catcttccac ctggaaccag gcgcgattcg
     3781 gctccgttcc gctgctgact ttgatccagc cattgccata acgggcggcg ttgaccacca
     3841 tattcgccac cgcgcgtttg atcgacagcg ggtgcatttt cacttcaatg ctgccggggt
     3901 aaagcgcggt ttcaatttcc cgctcatagc cactttcggc agcaatcacc tcaccgagta
     3961 ctgcattaag atccgccatt tccatcggca tctcctgccc ggtgcgcagg tagtcgataa
     4021 actgctcaat gatggcgttg cactcttcga tatctttatt gatcgattct gccagatagc
     4081 catcctgctc gctcatcatc tcagtcgcca ggcgaatacg cgtcagcggc gtgcgcaagt
     4141 cgtgacttac ccccgccatc agcagcgtgc ggtcatccgc cagttgctta acaccagccg
     4201 ccatatggtt aaaggcacgg gtaacggaac gcacctccga agcgccatat tcacgcagcg
     4261 gcggcggaat aatcccttta ccaacctgca aggctgcgtg ttcgagatcg accaacggtc
     4321 ggttctggat acgaataaac agccacgccc cgcctatcgc caatagcata atcgccagcg
     4381 tatagcggaa cagcggagag aaatcgccct gatgaatttc ggtcagcggc acgcgtaccc
     4441 agatattggg cgacagccag gttttcagcc agacgacagg cgaacttttg ttgacctcaa
     4501 cgcgcacttc cgtcgggccg cccagttgct gtgccatctg atggcttaag aattcatagt
     4561 gttgcgccca acgcagacct gcctcttcgg cagcctcgtt ggagtagaga gagatcccca
     4621 gctcacggta gatctcccga cggaaagcgg gaggcacaac caactgcgtg ccgtcctcca
     4681 gttgcagttt gtcggtcatc aacatacgca cttcgtacgc gaggacttta ttaaactgct
     4741 ggaggctcgg caaaatcgcg aagttcagca ccaccagata agtcgtcacc aggctggcga
     4801 acagcaaggt gacgatgagc aataacgtac gggcaaatga acttcgtggc gagaagcgca
     4861 atcgcctcat gctttagagc cgtccggtac aaagacgtag cccaggcccc aaacggtctg
     4921 aatgtaacgc ggatgcgctg gatcttcttc taccatgcgg cgcagacgcg aaatctgcac
     4981 gtcgatggag cgttccattg cggaatattc acgaccacgg gcaaggttca tcagcttatc
     5041 gcgggagagc ggctcacgcg gatggctgac cagtgccttc agtaccgcaa actcaccgct
     5101 ggtgagcggc atcggctcat cttcgcggaa catttcgcgc gtaccgaggt taagtttgaa
     5161 cttaccgaaa gcaattaccg cctcttcctg tgacggtgcg cctggcagtt cgttcgcctg
     5221 acgacgcagc accgcacgaa tacgggccag cagttcacgc gggttaaacg gttttggaat
     5281 gtagtcgtca gcgccaatct ccaggcctac gatacggtcc acttcttccc ctttcgccgt
     5341 caccataatg atcggcatcg ggttgctctg actacgaaga cgtcggcaaa tcgacaagcc
     5401 atcttcacca ggtaacatta aatccagtac cataagatgg aaagattcac gagtcagcag
     5461 gcgatccatc tgttctgcat tagcgacgct tcgaacctgg aagccttgtt cggtgagata
     5521 acgttccagc agcgcacgca ggcgcatgtc gtcatcgacc accagaatct tgtagttctc
     5581 ttgcattgtt tgtactccca aaggttcgca acaatttgta agcgtgtatt cttaaaaaag
     5641 ctcacgttcg tcaccagcta aatctggtat gaatttcagc ctaaattgtt acaaagcata
     5701 ttaaacagca gcttaagtat acaatttatt cggcgaaaca ttattgattc tgttgatatg
     5761 atcacgttat acccaatgtg cgcattatca aacagacaaa gggaatcaac gagatgaaaa
     5821 cgcccctggt tacccgggaa gggtatgaaa aactcaaaca agagcttaat tatctctggc
     5881 gtgaagaacg cccggaggtc acaaaaaagg tgacctgggc cgcaagtctg ggcgaccgca
     5941 gcgaaaatgc tgactatcag tataataaaa agcgtctgcg tgaaatcgac cgtcgcgtgc
     6001 gctatctcac taaatgcatg gaaaatctca aaatcgtcga ttactcccct cagcaggaag
     6061 gcaaagtctt ttttggcgcg tgggtggaga ttgaaaacga cgatggcgtg actcaccgtt
     6121 tccgtattgt cggctacgat gaaatttttg gccgtaaaga ttacatctct atcgattccc
     6181 cgatggcccg cgcattgctg aaaaaagaag tcggcgatct ggcggtggtg aatacccctg
     6241 ccggggaagc gagctggtat gttaatgcta tcgagtacgt gaaaccgtaa ggaagagtct
     6301 taacctcctg ccgatggctg gcatttttgc cagccagtcc gtataactat cccctgattt
     6361 ttgatccgaa aagatgaact caaaccatga tgaatgattc gttctgccgc attattgcgg
     6421 gtgaaattca ggcgcgcccg gaacaggttg acgctgccgt tcgcctgctt gacgaaggga
     6481 ataccgtgcc gtttatcgca cgttatcgta aggaaatcac cggcggtctg gatgacacgc
     6541 agctgcgtaa tctggaaacg cgtctgagct atctgcgcga gctggaagag agacgtcagg
     6601 cgatcctcaa atccatttcc gagcaaggca aactcaccga tgatctggcg aaggccatca
     6661 acgccaccct aagcaaaacc gaactcgaag acctctacct gccctacaaa cctaaacgcc
     6721 gcacccgcgg gcaaatcgcc attgaagcag ggcttgagcc gttggctgac ctgctgtgga
     6781 gcgatccgtc acacacgcca gaagtcgccg ctgcacaata tgttgatgcc gataaaggcg
     6841 tggcagatac caaagccgcg ctggatggcg cgcgctatat cctgatggaa cggtttgccg
     6901 aagatgccgc gctgctggcg aaagtgcgtg attatctgtg gaagaacgcg catttggttt
     6961 ctacggtggt gagcggtaaa gaagaggaag gggcgaaatt ccgcgactat ttcgatcatc
     7021 acgaaccgtt gtccacggtg ccttctcacc gcgcgctggc gatgttccgt gggcgtaacg
     7081 aaggcgtact ccagctttcg ctgaatgccg atccgcagtt cgatgagccg cccaaagaga
     7141 gctattgcga gcaaatcatc atggatcacc ttggcctgcg cctgaacaat gccccggcgg
     7201 atagctggcg caaaggcgta gtgagctgga cctggcgcat caaggtgctg atgcatctgg
     7261 aaaccgaact gatgggtacc gtgcgcgaac gcgcggaaga tgaagcaatc aacgtctttg
     7321 cccgtaacct gcacgatctg ctgatggcgg cccctgccgg actgcgtgca acgatgggcc
     7381 tcgatccggg tctgcgtacc ggggtaaaag tggcggtggt cgatgccact ggcaaactgg
     7441 tggcgaccga taccatttac ccgcacaccg gacaagccgc aaaagcagcg atgactgttg
     7501 ctgcgctgtg tgaaaagcat aacgttgaac tggtggcgat cggcaacggt acagcttccc
     7561 gcgaaaccga acgtttctat ctcgacgtgc agaagcagtt cccgaaagtg accgcacaga
     7621 aagtgatcgt cagcgaagct ggcgcgtcgg tttactcggc ttccgagctg gcagcgcagg
     7681 agtttccgga tctcgacgtt tcgctgcgtg gcgcggtgtc tatcgcccgc cgtttgcagg
     7741 atccgctggc ggagctggtg aaaatcgatc cgaaatctat cggcgtaggt cagtatcagc
     7801 atgacgtcag ccagacgcaa ctggcccgca aactggacgc agtagtagaa gactgcgtaa
     7861 acgccgttgg cgtcgatctc aacactgctt ctgttccgct gttaacccgc gtggcgggcc
     7921 tgacgcgcat gatggcgcaa aacatcgtgg cctggcgcga tgagaacggt cagttccaga
     7981 accgtcagca actgctgaaa gtgagccgtc tggggccgaa agccttcgag cagtgcgcgg
     8041 gcttcttgcg cattaaccat ggtgataacc cgctggacgc gtctaccgtc cacccggaag
     8101 cctatccggt ggtggaacgc attctggcag caacacagca ggcactgaaa gatctgatgg
     8161 gtaacagcag cgaactgcgt aacctgaaag cgtctgactt tactgatgaa aaattcggtg
     8221 tgccgacagt aactgacatc atcaaagagc tggaaaaacc gggtcgcgat ccgcgtccgg
     8281 aatttaaaac cgctcagttt gccgatggcg tcgagacaat gaacgacctg caaccgggta
     8341 tgatcctcga aggtgcggta accaacgtca ccaactttgg cgcgtttgtc gatatcggcg
     8401 tgcatcagga cggcctggtt cacatctctt cattgtcgaa caagtttgta gaagatccgc
     8461 ataccgtggt gaaagcgggc gacattgtga aggtgaaagt gctggaagtg gatcttcagc
     8521 gtaaacgtat cgccctgacc atgcgtctgg acgagcagcc tggcgaaacc aacgcccgtc
     8581 gcggcggcgg taatgaacgc ccgcaaaaca accgcccggc agccaaaccg cgcggacgtg
     8641 aagcgcagcc tgccggtaac agcgcgatga tggacgcgct ggcggcagca atgggcaaaa
     8701 aacgttaaac gccctgattg ccccctaaac ctgcgcgata aggtgtccac gccgcatctg
     8761 gcaacgaacg ccttgtctga cataaaagtg ccggagaata tctccggcat ttttattcca
     8821 cagccaaact cacaatatat tccggcaaca tttatcactt cattaacaac tgaaacctta
     8881 attaaacatt agccagtcct ggttattcgc tattcgaatt atattttcgc tgcgatataa
     8941 ccttgagcca catcaacatt gagtcagatt attattcaaa ccaacattcg cacacatttt
     9001 aagtattgct gatagaaacc attctcatta tcattgtgct gttgattatt taatctctcc
     9061 ttcgttggca aatcatctgg tctcatgtcg ctgtcaaacg ccccatgagg tagttatcca
     9121 gttaatgaga aacaagtagg cacctatgca atacactcca gatactgcgt ggaaaatcac
     9181 tggcttttcc cgtgaaatca gcccggcata tcgccaaaaa ctgctttctc taggcatgtt
     9241 acctggctcc tcttttaatg tggtgcgcgt cgctccactc ggcgacccca ttcatatcga
     9301 aacccgtcgt gtgagcctgg tattacgcaa aaaagatctg gccttattag aagtggaagc
     9361 ggtttcctgt taatacggtg ataacaacaa tgaaaaaatt aaccattggc ttaattggta
     9421 atccaaattc tggcaagaca accttattta accagctcac tggcgcacgt cagcgtgtag
     9481 gtaactgggc tggcgttacc gtcgaacgta aagaagggca attctccacc accgatcatc
     9541 aggtcacgct ggtggacctg cccggcacct attctctgac caccatctca tcgcagacct
     9601 cgctcgatga gcaaatcgcc tgtcactaca ttttgagtgg cgacgccgac ctgctgatta
     9661 acgtggtgga tgcgtctaac cttgagcgta acctgtacct gacgctacaa ctgctggaac
     9721 tcggcattcc ctgcatcgtg gcactgaaca tgctcgacat tgccgagaag caaaatattc
     9781 gtattgaaat tgatgctctg tcggcgcgtc tgggctgccc agtgatcccg ctggtttcaa
     9841 cccgtggtcg cggtattgaa gcgctcaagc tggcgattga tcgctataaa gctaacgaga
     9901 atgtggaact ggtgcattac gcacagccgc tactcaacga agcagattca ctggcaaaag
     9961 tgatgccttc cgacatcccg ctgaaacaac gtcgctggct gggcctgcaa atgctggaag
    10021 gcgatatcta cagccgcgcc tacgccggtg aagcgtcgca gcatctggat gccgccctcg
    10081 cccgtctgcg taatgagatg gacgatccgg cgctgcacat tgccgatgcg cgttaccagt
    10141 gcattgctgc catctgtgat gtggtaagca acaccctgac ggcagaaccc agccgtttca
    10201 ccactgcggt agataaaatc gtgctcaacc gtttcctcgg tctgccgatt ttcctctttg
    10261 tgatgtacct gatgttcctg ctggctatca acatcggcgg ggcgttacag ccgctgtttg
    10321 acgtcggctc cgtggcgcta tttgtgcatg gtattcaatg gattggctac acgctccact
    10381 tcccggactg gctgactatc ttcctcgccc agggcctggg tggcggcatt aacaccgtgc
    10441 tgccactggt gccgcagatt ggcatgatgt acctgttcct ctccttcctt gaggactccg
    10501 ggtatatggc gcgtgcggcg tttgtgatgg accgtctgat gcaggcgctg ggcttgccgg
    10561 ggaaatcctt tgtgccgctg atcgtcggtt tcggttgtaa cgtaccgtcg gtaatgggtg
    10621 cacgtacgct tgatgcaccg cgtgaacgtc tgatgaccat catgatggca ccgtttatgt
    10681 cctgcggcgc gcgtctggct atcttcgcag tattcgcggc tgccttcttc gggcagaacg
    10741 gtgcgctggc ggtcttctcg ctgtatatgc tgggtattgt gatggcggtg ctgactggcc
    10801 tgatgctcaa gtacaccatc atgcgcggtg aagcgacgcc gtttgtcatg gagttgccgg
    10861 tctatcatgt accacacgtt aaaagcctga ttatccagac ctggcagcgt ctgaaaggct
    10921 tcgttctgcg tgctggtaaa gtgatcatca tcgtcagcat tttcctgagc gctttcaaca
    10981 gcttctcgct gagcgggaaa atcgtcgata acatcaacga ctcggcgctg gcgtccgtca
    11041 gccgggtgat caccccggtc ttcaagccaa ttggcgtgca tgaagataac tggcaggcaa
    11101 cggttggcct gtttacaggt gccatggcga aagaagtggt agtgggtacg ctcaacaccc
    11161 tctacaccgc agaaaatatt caggacgaag agttcaatcc ggcagagttt aacctcggtg
    11221 aagagctgtt cagtgcgata gatgaaacct ggcagagcct gaaagacacc ttcagcctta
    11281 gcgtactgat gaaccccatt gaagccagca aaggcgacgg cgaaatgggt accggggcga
    11341 tgggagtgat ggatcagaaa ttcggtagcg cagcagcagc ttacagctac ctgattttcg
    11401 tcctgctgta tgtaccatgt atctcggtga tgggagccat cgcccgtgaa tcaagccgtg
    11461 gctggatggg cttctccatc ctgtgggggc tgaatatcgc ttactcgctg gcaacattgt
    11521 tctatcaggt cgccagctac agtcagcacc caacttacag cctggtgtgc attctggcgg
    11581 ttatcctgtt taacatcgtg gttatcggtc tgctgcgccg cgcgcgtagc cgggtggata
    11641 tcgaactgct ggcaacccgc aagtcggtaa gcagttgctg cgcagccagc accaccggtg
    11701 attgccatta atggcttcac ttattcaggt gcgcgatttg ctggcgttac ggggccgtat
    11761 ggaagcggcc cagataagcc agacattgaa cactccacag ccaatgatta acgccatgct
    11821 gcaacaactg gaaagtatgg gcaaagccgt gcggattcag gaagaacctg acggctgcct
    11881 ctctggcagt tgtaaaagct gcccggaagg aaaagcctgt ctgcgcgagt ggtgggcgct
    11941 gcgttaacct tactccatcg cctgttttgg aaagcccggt atgcgtctgc atccgggctt
    12001 ttttgcgtgc ggctttccat aaaaatgcaa ctcttgcagc acggcgtaag ttcctttgaa
    12061 agcatctcgc agggatgaaa actcgctaat acacaggtgt ggagtggcgc gtagagtcgc
    12121 ggcattcaaa caacaggtga aggaacgcca tgagcaaaaa gcagagttcc accccacacg
    12181 atgcactgtt caaactcttt ttacgccaac cggacacggc tcgtgatttt cttgcgtttc
    12241 atttaccggc acccattcac gcgctttgtg atatgaaaac cctcaagctg gagtcgagca
    12301 gctttattga tgacgatctg cgtgaaagct attccgatgt gctgtggtcg gtgaaaacgg
    12361 aacaaggacc aggatacatc tattgtctga ttgaacatca aagcacctca aacaaactga
    12421 tcgcatttcg catgatgcgt tacgctattg ccgcaatgca aaatcacctt gatgctggat
    12481 acaaaacgtt gccgatggtg gtgccattgt tgttttacca cggtattgaa agcccctatc
    12541 cctattcgct gtgttggctg gattgtttcg ccgatcccaa actggcaagg cagctttatg
    12601 cctccgcatt tccgctgatt gatgtcaccg tcatgcctga tgatgaaatc atgcagcacc
    12661 gacgtatggc gctgctggag ttaattcaaa aacatattcg tcaacgcgat ctgatggggc
    12721 tggtagagca aatggcctgc ttattaagta gtggatacgc taatgacaga caaatcaaag
    12781 ggctgtttaa ttacatactg caaaccggcg acgcggtacg ttttaacgat tttatcgacg
    12841 gcgttgccga acgttcaccg aaacacaagg agagtttaat gacaattgcg gaaagattgc
    12901 ggcaggaggg ggaacaatcc aaagccctgc atatagccaa aataatgctt gaatccggag
    12961 tccctcttac agacatcatg cgctttaccg gggtgtcaga agaagagttg gctgcggcga
    13021 gccagtaaat ttctgtctcg ccatttcaaa agttgcctac accctctgct tcaacgccac
    13081 cagcaggtga caaaactcga ccggatgcga aataaacggc gcatgggccg ctttggcgaa
    13141 gatatatgat tcgctgtgag gccaaagttt atccagcatc ggcaccactt tgcgcggcac
    13201 cagaccgtcg agatagccat acaatcgcaa aaacggcatg gacacgtttt gcagcggctg
    13261 acggagatcg accgttttca ggatttccag cccgccatta agcacgtcaa cctccggcat
    13321 cggtaacgcc agaacggttt tcttcaacgc ccgcacatcc tggcgcgccg tttcagtccc
    13381 catggtttgt aacgccagga accgctccac tgtacgctga aaatcatcac tgagttgctg
    13441 ctgaaatccc gccagcacgt ccggttttat ccccggccac tcgtcacgag cactaaaaca
    13501 aggtgacgac gccacggtga ccagcgcctg aacacgctcg ggatgggtta aaacaatttg
    13561 gcttgccacc agcccgccca gactccagcc taaccaaatg gctttatcag gtgcctgttg
    13621 cagcacggct tcggccatat cagcaagtga cagcgcacca aatccccggc tacgcccgaa
    13681 gccgggcagg tcaacaaggt gcagcgtaaa atgcgagcta agttcctcgt caatgcaacg
    13741 ccacacttcg gcattcagtc cccatccgtg cagcagcaca agatgaacat tcccctgacc
    13801 tttggtctgc caccagatgt tattcatccg ctattgttct cttttgactt acaaggatga
    13861 acatatgcta acagtaccgg gattatgctg gctatgccga atgccactgg cgttaggtca
    13921 ttgggggatt tgttcggtct gttcacgcgc cacccgcaca gataaaacgt tatgcccaca
    13981 atgtggatta cccgccacac actcccatct tccctgcggt cgctgcctgc aaaaaccgcc
    14041 gccctggcaa agactggtca cggttgccga ctatgcgccg ccgttaagtc cgcttatcca
    14101 ccagcttaaa ttttcccggc gcagtgaaat cgccagcgcc ctgtcacgtc tgttactatt
    14161 ggaagtctta cacgctcgtc gcaccaccgg gttgcaattg ccggatcgca tcatcagcgt
    14221 tccgttatgg cagcggcgtc actggcgtcg gggatttaat cagagcgatt tgctgtgtca
    14281 gccgttatca cgctggttgc actgccaatg ggatagcgaa gccgtcacac gtacacgggc
    14341 cactgcgacc cagcattttc tcagtgcccg gctgcgcaag cgcaacctga aaaatgcctt
    14401 tcgtcttgaa ttgcccgtgc aaggtcgcca tatggtgatt gtggatgatg tcgttaccac
    14461 cggaagtacc gtcgcagaga ttgcgcagtt gcttttacgc aatggtgcgg cgactgtcca
    14521 ggtctggtgc ctttgtcgaa ccttgtagag cctcgatgat gggcgtatta taaccaacta
    14581 aaatagtcaa ctattaggcc attactatga tccgtatttc cgatgctgca caagcgcact
    14641 ttgccaaact gctggcaaat caggaagaag ggacacaaat ccgcgtattt gtgattaacc
    14701 ctggcacgcc taacgctgaa tgtggtgttt cttattgtcc gccggatgcc gtggaagcca
    14761 ccgacactgc cctgaaattt gacctgctga ccgcgtatgt tgatgagtta agcgcaccat
    14821 acctggaaga tgcagagatc gactttgtta ccgaccagtt gggttctcag ttaacgctga
    14881 aagccccgaa cgccaaaatg cgtaaagtgg cagacgatgc accgctgatg gagcgcgtgg
    14941 agtatatgct gcagtcgcag atcaacccac agcttgctgg tcacggtggt cgcgtttcgc
    15001 tgatggaaat caccgaagac ggttacgcca ttctgcaatt tggcggcggc tgtaacggtt
    15061 gttccatggt cgatgtgacg ctgaaagaag ggatcgagaa gcagctgctg aacgaattcc
    15121 cggagctgaa aggtgtgcgc gatctcaccg aacaccagcg cggcgaacac tcctactact
    15181 aagttatccc ctcttttgta gattgcccga tgcgacgcta aagcgtcatt tcgggcctac
    15241 gttgaacatg tgccagacgt tggcacagca ttgctttggc aaatccctcc cttcgtttcc
    15301 ccgcgataat atgaccaacc tctcataatt taaatttacc ccgctctggt gattctcaaa
    15361 cgccagatgt tacccgtatc attcacatgg gtaccaaaca tactcctgac atctgactac
    15421 aataattagt tttagtgggt atcagtcgtg gtgccgcaat atctctgttc ccgattggga
    15481 taattagagt ttgtcgtcag aaaattgacg ttacccataa caaatgaaag gccaggtaaa
    15541 tcatgccatt agtcattgta gctatcggtg taatcttgtt gttgctcctg atgatccgct
    15601 tcaaaatgaa cggcttcatc gctctcgtcc tcgtggcgct tgctgttgga ttaatgcaag
    15661 gaatgccgct ggataaagtt attggctcca tcaaagccgg tgtcggcggg acgctcggta
    15721 gccttgccct gatcatgggt tttggcgcaa tgctgggcaa aatgctggca gactgcggtg
    15781 gcgcacaacg tatcgccacc acgctgattg ccaaatttgg taaaaaacac atccagtggg
    15841 cggtggtact gaccggtttt accgttggtt ttgccctgtt ctatgaagtg ggctttgtgc
    15901 tgatgctgcc gctggtgttt accatcgcgg cttctgcgaa tattccactg ttgtatgttg
    15961 gtgtaccaat ggcagctgca ctgtctgtga cccacggctt cctgccaccg catccgggtc
    16021 cgactgcgat tgccaccatt ttcaatgccg atatgggtaa aaccctgctg tacggtacta
    16081 ttctggcaat cccgaccgtg attctcgccg gtccggttta cgctcgcgtg ttgaaaggta
    16141 tcgataagcc aatcccggaa ggtctctaca gcgcgaaaac cttcagcgaa gaagagatgc
    16201 cgagctttgg cgtcagcgtc tggacttctc tggtgccggt tgtgctgatg gcgatgcgtg
    16261 cgattgccga aatgatcctg ccgaaaggtc acgctttcct gccggtagcg gagttcctcg
    16321 gtgacccggt aatggcaacg ctgattgccg tgctgattgc gatgttcacc tttggtctga
    16381 accgtggtcg ttcaatggat cagattaacg acacgctggt ttcttccatc aaaatcattg
    16441 cgatgatgct gttgatcatc ggtggtggcg gtgcgttcaa gcaggtgctg gtagacagcg
    16501 gcgtggacaa atacattgct tccatgatgc acgaaaccaa catttctccg ctgctgatgg
    16561 cctggtcgat tgctgccgta ctgcgtatcg cgctgggttc cgcaaccgtt gcggcaatca
    16621 ctgcgggtgg tatcgcggca ccgctgattg caacgacggg tgttagccca gagctgatgg
    16681 ttattgcggt gggttccggt agtgtgattt tctctcatgt gaacgatccg ggcttctggc
    16741 tgttcaaaga gtactttaac ctgactatcg gcgagaccat caaatcctgg tcgatgctgg
    16801 aaaccattat ctcggtgtgc ggtctggtag gctgtctgct gctgaatatg gtgatttgat
    16861 aacactgact gccggatgca tcgcatccgg catcaaccgc actctacttc ttcttcgctg
    16921 cagctctgcg ccgtctgtcc aaatccttca gcaacttgtt cactccatca tcggcaaaca
    16981 tcgactcaag cgttgcggaa agcttgcgtc gccagttttt atactggtaa ctggtgccag
    17041 gaatattcac cggttcggcc atatccagcc agtcttccgg ctgtagtcct aacagagcac
    17101 tgttactgtc ggcaatgtag cgctgcaaac cacggttcag cgtcggcgtc atcgacatca
    17161 acgatgcctt atgcccggca cgttttggca gacaaccata tttatgcagt gcatccagca
    17221 gcccttgctt cgccagttcg cgatcctgat acagaccgcg cagtaccact tcatccggat
    17281 acagccccag ggttttgccc agcgttagat ccccgctctc ccagtaaccg cgtagcgtag
    17341 gcaggtcatg tgtcgccgca accgccatcg actgctccgg atacgctttc ggtgcacgga
    17401 acgtcttctc gtggtcgttt tcgaaataga gcactttgta ggagtacaca ccgctgctgc
    17461 gcagcttacc gacaatctct accggtacgg taccgagatc ttcaccaatc accatacagc
    17521 gatgacgttt actttcgagt gccagaatcg agagcagatc atccaccgga tagtgaacat
    17581 acgcgccctg atctgccgtc tcgccatacg gtatccacca caaacgcagc atcgacatca
    17641 catggtcaat tcgtaatgcg ccgcagtttt gcatattggc acgcaacagc tcgataaacg
    17701 gttcataggc acgcgcggtg atgatatgcg ggtccattgg cggtaatccc cagttctgcc
    17761 ccaacgggcc gaggatatcc ggcggcgcgc caaccgacgc tttcaggcaa tatagttcac
    17821 ggtcacacca ggtttccgcc ccaccttccg ctacgccaac cgccagatca cgatacaagc
    17881 caatcggcat ttcatagccc tggcttatct cccagcaggc ggcaaactgg ctgtaagcca
    17941 gccactgcaa ccagagataa aaatcgacgt cattacgatg ttcttcgcag aactgacgca
    18001 cttctggtga atccacgttc tgatacatct ctggccatgc aggccagccc cagcgcattt
    18061 cgtcctcttt cacttgctgg gcatgtagcg catcaaaggc tgcctgccag aacaggctgt
    18121 cgccctgctc tgcaacaaac tggcgaaacg cggccatctg ctcatcatca cgttgcgcga
    18181 aacctttcca cgccattcgt aatgctgtca tttttagggc ggtaaccgtg gagtaatcga
    18241 cccagtcggc atcgcgcgcc tgttgcagcg tctgttgcgt ggtcggcaac tgccaccagg
    18301 cctgagcctc ttcgctaaga tggaaatctt cgacggcgtt aacgtcgata taaatcacat
    18361 tcagccaacg gcgagaagac gggctgtatg ggctggcact ctccggattt gccggataga
    18421 gcgcatgaat cgggttcagg ccgatgaacg acccgccacg ttttgccaca tccaccagca
    18481 tcgctttgag atcgccaaaa tccccaatac cccagttttt ttccgatcgc agcgtataaa
    18541 gctgaacgca ggcaccccac agcttttgtt tattcagcaa cgcctgcggt tcgtaacagc
    18601 gtttcggggc gacaatcacc cggcaatgcg cgcgctggtc gtcctgggtg agtgtcagcg
    18661 tgtgataacc ttccggcagc ttcgtcggta gattgaacgc tttgcccccc gttacatggc
    18721 ctttgtactg tgttccttct tcggtggtca gcagccagct atattcgccg ctgccctcca
    18781 ccaccatcgg catttttttg ccgctggtat aaaccatgac attcgggact ggcgttaccg
    18841 ccactttcgt ggcggtacgt tgatgcatcg cgtcaagcaa acgccgtttg gtttcggcgc
    18901 taatcgactg cggtttaccg tgggcattga tgtaattggg gctaatcccc gccgccagcg
    18961 cggcattatc cagacgtttg ctttccatcg agcttcctta gcgttttgcc tgccagatac
    19021 gagcctgata atcgcgaata gagcgatccg agctaaacat accgcagcgg gcggtattga
    19081 ggatcgccgc gcgagtccag gcctcctggt cgcggtacag cacatccacc tgcttttgtg
    19141 cctcaacata ggctgcgaaa tccgccatca ccagatacgg atcgccgcct tgtttgccga
    19201 tactgtgcag catctggtcg aaggcatgct tatcgccgtc gctgtattta ccgctttcca
    19261 gctctttcaa tactgcgtcc agcaccttat ctttcttccg ccatttcacc gggtcgtagc
    19321 ctttggccag aatagccttc acttgttcca cggtatgacc aaaaataaag atattttctt
    19381 caccgacttt ctcggcgatt tcaacgttcg caccatccag cgtaccgaca gtaagcgcac
    19441 cattgagcgc cagtttcata ttgccggtac cggaagcttc tttacctgca gtcgaaattt
    19501 gttcggagat atccgccgcc gggatcagtt tttccgccgc cgaaacgcaa taatccggca
    19561 ggaacaccac cttcaactta tcgccaacca gtggatcgtt gttgatcacg tcagccactt
    19621 tgttgatcgc aaagataata ttcttcgcca ggtagtagcc cggtgccgct ttcgcgccga
    19681 agaggaagac gcgcggtacg cgatcagcct gcgggttttc acgaatttct ttgtacaacg
    19741 ccagaatatg cagcagattc aggtgctggc gtttgtactc atgcagacgt ttgatctgaa
    19801 tatcgaaaat cgcctgtgga ttgatctcaa taccggtacg aactttcaca aactccgcca
    19861 gacggacttt attcgcctgc ttgatctcgc gatattgctg acggaatttc gcatcatcag
    19921 cgaatttttc cagattgatc agctgatcga gatcgttagc ccactctttt tgcagtgatt
    19981 tatccaacag agccgccagt gcagggttgc actgtttgat ccagcgacgt ggggtaatac
    20041 cgttggtgac gttatggaat ttgttcggcc atagctggtg atattccggg aacagatctt
    20101 tcaccaccag atccgagtgc agcgccgcaa caccgttcac cgcgaaaccg ccaaccacac
    20161 acaggttcgc catatgcact tgtttatcgt gcaccaccgc cagtttggcc cacacttttt
    20221 catcgcccgg ccaggttttc tccaccagcg ttttaaagcg agtattaatt tcgttaataa
    20281 tctgcatatg gcgcggcagt aagcctttca ccagtttcac atcccagcgt tccagcgctt
    20341 ctggcatcag ggtatggttg gtgtaagcga aagttttgct ggtaatggcc caggcgtcat
    20401 cccagctcat ctggtgctca tcgatcagca cgcgcagcag ttctggaatc gcgatagttg
    20461 ggtgggtatc gttcagctga ataacttcgt aatccgccag ttcgtgcagt ttacgccccg
    20521 ccagatgatg gcgacgcaaa atatccgcta ccgaacaggc acactggaag tactgctgca
    20581 tcaagcgcag ctttttaccg gcagtatggt tgtcgtttgg atagagaact ttggtcagtt
    20641 tttccgcatt gatgccctgc tgttcggcac gcaggaaatc accgtcgtta aatttagtca
    20701 gatcaaacgg atgcgcgtgc gtcgcctgcc acagacgcag cggctgcgcc acgccattac
    20761 gatagccgac aacggggaga tcccacgctt gaccggtaat ggtaaactcc ggctcccagc
    20821 gtccgtcttt cgtcacttta ccgccaatcc ctacctgcac atccagtgct tcgttgtggc
    20881 ggaaccacgg gtagttactg cgatgccagt catccggcgc ttcaacctgt ttgccatcga
    20941 caaaagactg gcggaacaaa ccatattgat agttcagacc gtaacccgtc gcagactgac
    21001 cgacagttgc cattgagtcg aggaagcacg ccgccagacg tcccagacca ccgttaccca
    21061 gcgccgggtc gatctcttct tccagcaggt ccgtcagatt gatgtcataa gccttcaacg
    21121 aatcctgtac atcctgatac cagccgagat tcaacaggtt gttgcccgtc aggcgaccaa
    21181 tcaaaaactc cattgagatg tagttaacat gtcgctgatt cgccaccggc ttggcgaatg
    21241 gctgagcacg cagcatttcg gccagtgctt cactcactgc tagccaccac tggcgaggag
    21301 tcatttcagc cgcagaattt aagccataac gctgccactg acgtgaaagc gcttcctgaa
    21361 attgcttatc gttaaaaata ggttgtgaca taggagttcc acttttctta gattttcaac
    21421 acaacgttat cgctagtttg ccaggctcga tgttgacctt cctcatcctg cgggggatta
    21481 ggcagggagg agttgcgggg atgagcaagg aaatgtgatc tcaaccactt aaagctagtg
    21541 caaaccacag gattagcagc aaatcaatgc aatacagcgc agaaaatctg tatctaagtg
    21601 caaaaaatgg ccgttgcgta ttttcaaaaa gcggaaggta actctataaa ttaagtaaag
    21661 gagtgaaaca gtttcataag taaaatatcc agtgtgctcc atctcattct taatagattt
    21721 attaagatca tctttttaga tagcactttc atcaggaatg aagaagaaac ccttgcttaa
    21781 atgaatctga tgaacataag ggaaaccagt attcacgctg gatcagcgtc gttttaggtg
    21841 agttgttaat aaagatttgg aattgtgaca cagtgcaaat tcagacacat aaaaaaacgt
    21901 catcgcttgc attagaaagg tttctggccg accttataac cattaattac gaagcgcaaa
    21961 aaaaataata tttcctcatt ttccacagtg aagtgattaa ctatgctgat tccgtcaaaa
    22021 ctaagtcgtc cggttcgact cgaccatacc gtggttcgtg agcgcctgct ggctaaactt
    22081 tccggcgcga acaacttccg gctggcgctg atcacgagtc ctgcgggcta cggaaagacc
    22141 accctcattt cccagtgggc ggcaggcaaa aacgatatcg gctggtactc gctggatgaa
    22201 ggtgataacc agcaagagcg tttcgccagc tatctcattg ccgccgtgca gcaggcaacc
    22261 aacggtcact gtgcgatatg tgagacgatg gcgcaaaaac ggcaatatgc cagcctgacg
    22321 tcactcttcg cccagctttt cattgagctg gcggaatggc atagcccact ttatctggtc
    22381 atcgatgact atcatctgat cactaatcca gtgatccacg agtcaatgcg cttctttatt
    22441 cgccatcaac cagaaaatct caccctggtg gtgttgtcac gcaaccttcc gcaactgggc
    22501 attgccaatc tgcgtgttcg tgaccaactg ctggaaattg gcagtcagca actggcattt
    22561 acccatcagg aagcgaagca gttttttgat tgccgtctgt catcgccgat tgaagccgca
    22621 gaaagcagtc ggatttgcga tgacgtttcc ggttgggcga cggcactaca gctaatcgcc
    22681 ctctccgccc ggcagaatac ccactcagcc cataagtcgg cacgccgcct ggcgggaatc
    22741 aatgccagcc atctttcgga ttatctggtc gatgaggttt tggataacgt cgatctcgca
    22801 acgcgccatt ttctgttgaa aagcgccatt ttgcgctcaa tgaacgatgc cctcatcacc
    22861 cgtgtgaccg gcgaagaaaa cgggcaaatg cgtctcgaag agattgagcg tcaggggctg
    22921 tttttacagc ggatggatga taccggcgag tggttctgct atcacccgct gtttggcaac
    22981 ttcctgcgcc agcgctgcca gtgggaactg gcggcggagc tgccggaaat ccaccgtgcc
    23041 gccgcagaaa gctggatggc ccagggattt cccagcgaag cgattcatca tgcgctggcg
    23101 gcaggcgatg cgctgatgct gcgcgatatt ctgcttaatc acgcctggag tttgttcaac
    23161 catagcgaac tgtcgctgct ggaagagtcg cttaaggccc tgccgtggga cagtttgctg
    23221 gaaaatccgc agttagtgtt attgcaggcg tggctaatgc aaagccaaca tcgctatggc
    23281 gaagttaaca ccctgctagc ccgtgctgaa catgaaatca aggacatcag agaaggcacc
    23341 atgcacgcag aatttaacgc tctgcgcgcc caggtggcga ttaacgatgg taatccggat
    23401 gaagcggaac ggctggcaaa actggcactg gaagagctgc cgccgggctg gttctatagc
    23461 cgcattgtgg caacctcggt gctgggtgaa gtgctgcact gcaaaggcga attgacccgc
    23521 tcactggcgc taatgcagca aaccgaacag atggcacgcc agcacgacgt ctggcactac
    23581 gccttatgga gtttaatcca gcaaagtgaa attctgtttg cccaagggtt cctgcaaacc
    23641 gcgtgggaaa cacaggaaaa agcgttccag ctgatcaacg agcagcatct ggaacagctg
    23701 ccgatgcatg agtttctggt gcgcatccga gcacaactgt tatgggcctg ggcacggctg
    23761 gatgaagccg aagcgtcggc gcgtagcggg attgaagtct tgtcgtctta tcagccacag
    23821 caacagcttc agtgcctggc aatgttgatt caatgctcgc tggcccgtgg tgatttagat
    23881 aacgcccgta gccagctgaa ccgtctggaa aacctgctgg ggaatggcaa atatcacagc
    23941 gactggatct ctaacgccaa caaagtccgg gtgatttact ggcaaatgac cggcgataaa
    24001 gccgccgctg ccaactggtt gcgtcatacg gctaaaccgg agtttgcgaa caaccacttc
    24061 ctgcaaggtc aatggcgcaa cattgcccgc gcacaaatct tgctgggcga gtttgaacct
    24121 gcggaaattg ttctcgaaga actcaatgaa aatgcccgca gtctgcggtt gatgagcgat
    24181 ctcaaccgta acctgttgct gcttaatcaa ctgtactggc aggccggacg taaaagtgac
    24241 gcccagcgcg tgttgctgga cgcattaaaa ctggcgaatc gcaccggatt tatcagccat
    24301 tttgtcatcg aaggcgaagc gatggcgcaa caactgcgcc agctgattca actcaatacg
    24361 ctgccggaac tggaacagca tcgcgcacag cgtattctgc gagaaatcaa tcaacatcat
    24421 cggcataaat tcgcccattt cgatgagaat ttcgttgaac gtctgctgaa tcatccggaa
    24481 gtgccagaac tgatccgcac cagcccgttg acccaacgtg aatggcaggt cctggggctg
    24541 atttactccg gttacagcaa tgagcaaata gcgggtgaac tggaagtggc ggcaaccacc
    24601 atcaagacgc atatccgcaa tctgtaccaa aaactcggcg tcgcccaccg tcaggctgcc
    24661 gtgcagcacg cgcagaaact gctgaagatg atgggttatg gggtgtgagt ttagccggat
    24721 aatgcattgg atccggttta catctttaca tcattcaata ctcacccgcg ttacgccatc
    24781 tgtttctatc aaaccaaacc gcaccggcaa gaaacgctcc accaccgcga tattggtcag
    24841 cagatggcat gaaggatggg cgaccgtaaa ttctcccgcg cccgccagcg ccatcggcag
    24901 taccagctgg tcggcgagat attcccccac tgccgccggg cttgccaggt agcgtttcac
    24961 ctctttcact aactgcgcgg ctaccacctc ggcactgacg cgcttttcac cgacgacaaa
    25021 aaagcgttcg gtgatatttt cactttcgac ttcaagcgag acggtattac ccggcccctg
    25081 gtcacgcggc aggttatgaa tattctgctc atgcagggaa aaactacccg ccagcgtagc
    25141 gatttcacgc tcagcgacat ggcgcggcac gccagctaat aacacctctc cacgcatctg
    25201 cacaatgttc ccgcgctcgc caagttgcaa ggtgttaaac gatgccaccg gcgagacttc
    25261 cgttgccacc acgccgcctc cggcaggata aaaaccgtga cgtaacagcg tggtttgctg
    25321 atgaattcct attttcgcca gcagcggctc cagcacccgg cggataaaat ccgcaggcgg
    25381 ggccgacggg ttatcggtgc ctccgctcac ttcaacacgc gaaggtccat cggcaaacca
    25441 cagcgcgggc agcaccgttt gcagcaccag cgtacagctt ccggcactac cgatagcaaa
    25501 gcggtactcg ccgccgcgca cggtgccagg ccggaagacc agacgctgcg accccagctc
    25561 cgcgccttcc accgttgccc tacaaatttc cgcagccgct tttaccgcgg tcagatgctg
    25621 gcgcaacagc cccggtttcg cccgtccggc tcgaatgcca gtgatggtaa atggctggcc
    25681 ggttatcatc gacaggctca gcgccgagcg caggatctgt ccgccgcctt cgccctgtgc
    25741 gccatccagc gcaatcatcc ttttcatcat ttatcctttt acgcacacca cctgacgcag
    25801 ggtgtagata acttccacca gatcgctttg tgccgccatc accgcatcaa tatctttata
    25861 cgccatcggg atttcgtcga tcacttcggc atctttacgg cattccacat gcgcggtggc
    25921 acgaatttga tcttccacgc tgaacagttt tttcgcttta gtacggctca ttacccgccc
    25981 ggcaccgtgg ctgcacgaac agaacgactc ttcatttccc agcccacgga cgataaagct
    26041 ttttgctccc atcgaaccgg gaataattcc atattgacca gcacgcgcag acaccgcgcc
    26101 tttacgcgtc acgtagatct cttcaccaaa gtgctgttct ttttgcacat agttgtggtg
    26161 acagttgatc tcttccatcg ccagcgtttg tggctgtctg accgttttct gcgtaatgct
    26221 ctgcaacgcc gttaccacat tttccatcat cgcatcgcgg ttaaggctgg cgaaaagctg
    26281 cgcccaggcc acggctctta ggtaatcatc aaagtattcc gtaccttcca taaagtacgc
    26341 cagatcacgc gacggcaacg tctcaagttg gtcctgcatc tctttttgcg ccagatcgat
    26401 aaagtaagtc ccgatggcgt taccaattcc gcgtgaaccg gagtgcagca taatccacac
    26461 ctgctccgac tcatcaaggc agatttcaat aaagtggtta ccggttccca gcgttcccag
    26521 gtgtttatag ttattggtat tcaggaaacg gggatatttt tgcgttaacc actgataacc
    26581 ggcttccagc tcagcccatt tcgcatcgac gttaacaggc gggttttccc aggcaccttt
    26641 atcacgttta caacggccag tggtacgccc gtgcggcacg gccgtttcaa tcgcctgacg
    26701 cagctctgcc aggttttcag gcaggtcttc cgccgttaac gcggtacgca gcgcgttcat
    26761 tccacagcca atatccacgc ccagcgccgc cggaataatc gcccctttgg tcgggatcac
    26821 gctaccaatg gtggaacctt tacccaggtg tacatcaggc attaccgcaa tatgtttgaa
    26881 aataaacggc atcttcgccg tattaataag ttgctgacgc gcatcggcct ctaccggcac
    26941 gcctttggtc cacattttta ccggggcatt ttcagtggtc agtaattcgt aattcatttt
    27001 gtttttctct tttcgttgtt tgctgtcctg ataattgcaa ccgtcgtgcc agaaaattaa
    27061 ataagcagct taatttttta att
//
LOCUS       DAZKIV010000051        26988 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZKIV010000051 DAZKIV010000000
VERSION     DAZKIV010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26988
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.051"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(114..1706)
                     /gene="lsrK"
                     /locus_tag="ACHOT6_002122"
     CDS             complement(114..1706)
                     /gene="lsrK"
                     /locus_tag="ACHOT6_002122"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFP9947342.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVVFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPLGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGVTTSLWKAPGL"
     gene            complement(1785..2738)
                     /gene="lsrR"
                     /locus_tag="ACHOT6_002123"
     CDS             complement(1785..2738)
                     /gene="lsrR"
                     /locus_tag="ACHOT6_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFP9947343.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVPDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAVAILRS"
     gene            2987..4522
                     /gene="lsrA"
                     /locus_tag="ACHOT6_002124"
     CDS             2987..4522
                     /gene="lsrA"
                     /locus_tag="ACHOT6_002124"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFP9947344.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISFDLEEIELMTDRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4516..5544
                     /gene="lsrC"
                     /locus_tag="ACHOT6_002125"
     CDS             4516..5544
                     /gene="lsrC"
                     /locus_tag="ACHOT6_002125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFP9947345.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5544..6536
                     /gene="lsrD"
                     /locus_tag="ACHOT6_002126"
     CDS             5544..6536
                     /gene="lsrD"
                     /locus_tag="ACHOT6_002126"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFP9947346.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6548..7570
                     /gene="lsrB"
                     /locus_tag="ACHOT6_002127"
     CDS             6548..7570
                     /gene="lsrB"
                     /locus_tag="ACHOT6_002127"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFP9947347.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELGVDVTYDGPTEPSVPGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            7597..8472
                     /gene="lsrF"
                     /locus_tag="ACHOT6_002128"
     CDS             7597..8472
                     /gene="lsrF"
                     /locus_tag="ACHOT6_002128"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFP9947348.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            8496..8786
                     /gene="lsrG"
                     /locus_tag="ACHOT6_002129"
     CDS             8496..8786
                     /gene="lsrG"
                     /locus_tag="ACHOT6_002129"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFP9947349.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            8843..9601
                     /gene="tam"
                     /locus_tag="ACHOT6_002130"
     CDS             8843..9601
                     /gene="tam"
                     /locus_tag="ACHOT6_002130"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFP9947350.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALRDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KHYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(9605..10519)
                     /locus_tag="ACHOT6_002131"
     CDS             complement(9605..10519)
                     /locus_tag="ACHOT6_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFP9947351.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(10726..12177)
                     /gene="uxaB"
                     /locus_tag="ACHOT6_002132"
     CDS             complement(10726..12177)
                     /gene="uxaB"
                     /locus_tag="ACHOT6_002132"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFP9947352.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFECFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(12404..13822)
                     /locus_tag="ACHOT6_002133"
     CDS             complement(12404..13822)
                     /locus_tag="ACHOT6_002133"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9947353.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRMLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSSMRYGEEVV"
     gene            complement(13961..14320)
                     /locus_tag="ACHOT6_002134"
     CDS             complement(13961..14320)
                     /locus_tag="ACHOT6_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFP9947354.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(14320..15246)
                     /gene="glsB"
                     /locus_tag="ACHOT6_002135"
     CDS             complement(14320..15246)
                     /gene="glsB"
                     /locus_tag="ACHOT6_002135"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFP9947355.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(15310..16698)
                     /gene="sad"
                     /locus_tag="ACHOT6_002136"
     CDS             complement(15310..16698)
                     /gene="sad"
                     /locus_tag="ACHOT6_002136"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFP9947356.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDHRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            16758..17645
                     /locus_tag="ACHOT6_002137"
     CDS             16758..17645
                     /locus_tag="ACHOT6_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9947357.1"
                     /translation="MNNIKPLIHEFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETE
                     LGVDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTA
                     AVRIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPV
                     YREELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEME
                     SYHGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRP
                     LEAFIQLLDVPDSAKQGYQ"
     gene            17723..18836
                     /gene="yneK"
                     /locus_tag="ACHOT6_002138"
                     /pseudo
     CDS             17723..18836
                     /gene="yneK"
                     /locus_tag="ACHOT6_002138"
                     /inference="COORDINATES: protein motif:HMM:NF033404.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            18986..20176
                     /gene="ydeA"
                     /locus_tag="ACHOT6_002139"
     CDS             18986..20176
                     /gene="ydeA"
                     /locus_tag="ACHOT6_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFP9947358.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(20201..20866)
                     /gene="marC"
                     /locus_tag="ACHOT6_002140"
     CDS             complement(20201..20866)
                     /gene="marC"
                     /locus_tag="ACHOT6_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFP9947359.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            21078..21512
                     /gene="marR"
                     /locus_tag="ACHOT6_002141"
     CDS             21078..21512
                     /gene="marR"
                     /locus_tag="ACHOT6_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFP9947360.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            21532..21915
                     /gene="marA"
                     /locus_tag="ACHOT6_002142"
     CDS             21532..21915
                     /gene="marA"
                     /locus_tag="ACHOT6_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFP9947361.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            21947..22165
                     /gene="marB"
                     /locus_tag="ACHOT6_002143"
     CDS             21947..22165
                     /gene="marB"
                     /locus_tag="ACHOT6_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFP9947362.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(22222..23660)
                     /locus_tag="ACHOT6_002144"
                     /pseudo
     CDS             complement(22222..23660)
                     /locus_tag="ACHOT6_002144"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            complement(23685..25358)
                     /locus_tag="ACHOT6_002145"
     CDS             complement(23685..25358)
                     /locus_tag="ACHOT6_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFP9947363.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(25414..25725)
                     /locus_tag="ACHOT6_002146"
     CDS             complement(25414..25725)
                     /locus_tag="ACHOT6_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFP9947364.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILGHAQDHLMNAILCLDLAREIISLRKELH
                     A"
     gene            complement(25753..>26988)
                     /locus_tag="ACHOT6_002147"
     CDS             complement(25753..>26988)
                     /locus_tag="ACHOT6_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFP9947365.1"
                     /translation="AVRDGFVFTFPIIMASSLIILINFAILSPDGFIAGLLHLNSIFP
                     NLEKAQAIFTPVMNGSVNIMSIMIAFLVARNMAISYEQDDLLCGLTAIGAFFIVYTPY
                     QMIDGQAFLTTKYLGAQGLFVAVIVALITSEIFCRLARNPKITITMPAVVPPAVARSF
                     KVLLPIFFVMVFFSALNYCLTLISPAGLNDLIYTLIQTPLKHMGTNIFAVIILGAVGN
                     FLWVLGIHGPNTTSAIRETVFSEANLENLSWAAQHGTTWGAPYPITWTSINDAFANCG
                     GSGMTLGLLLAIFIASKRAEYRDLAKMSFIPGIFNINEPIMFGLPIVLNPIMMVPFIM
                     VPIVNCAIGYFFVSMEIIPPVAYAVPWTTPGPLIAFLGTGGNWLALLVGFLCLGVATM
                     IYLPFVIAANKVNNMTTNG"
BASE COUNT         6983 a         6755 c         6399 g         6851 t
ORIGIN      
        1 aggatgaatc tgcaagctgg catttaacaa gccagctcta aaagaaggga aataagaata
       61 actatactca aaaactaaca gccacggtca tcatgatgtg gctgtcaatg aaactataac
      121 ccaggcgctt tccataacga cgtcgtcact ccatgatcaa ccagccccag ctgatcctga
      181 taaactgcct gccacttatc gcgtgaatcc tgataaagtt catgcttttc cgggtctggt
      241 gtgtgcgtcc gttcccagcg aaccaggcgt tctccggttt ctgccattga tgaaaaaatt
      301 ccggcaccga cgccagctgc aatggcacat cctaatgcgg tggcttcttt gactaccgga
      361 atattgacgg gtaatcccga gacatcagcg agaatttgac tccataattt cccttttgaa
      421 cctccgccag caaaaaccag cgaggtggga tgaatattcg agaaatcagc aatttgctgc
      481 aagttacacg ctgatacaat cgccgcattt tcttccagcg cacgaaacaa tgtcgctttg
      541 ttacatttat ccgggtcaat ggacaagtta ataaaggaag gtgcggcgtg ataccaggtt
      601 ttaaagcgca ttctgtcgga gaagatcggc attacgcccc acgacccaag cggcacccga
      661 ctggccatct cttccagcag cgtataggtg tcgatgccta aacgttcggc aatcagtttt
      721 tcttcggcac agaaagcatc gcggaaccag cgcatggtga gtccggtaaa aaagcttata
      781 gattcagctt gtaccatgcc aggaataaca tgaggattaa cgcgcacgtt catttctggg
      841 tctgtcaccg gcgcggctaa atttacaact tgctgccaga atgtgccgcc aagaaccgcg
      901 gtttgtgccg gacgcacaac gcctaaccca aggcaaccaa gctgcacgtc gcctcctcca
      961 acgaccaccg gagtgcccgc cttcagaccg cagagttccg ccgcttgtga acttaccacg
     1021 cccagcaatg tgccggtttc tttgacagga gaaagaatat cggcacgcag gccagccata
     1081 tccagcaatg caggtttcca gtcacgggtg gttagatcaa gaagtcccgt ggtgccagcg
     1141 ttagagggat ccaccgccag ttcgccgctg agcatatagg ccagccagtc gctgatcatg
     1201 gtgatggttg atgcctgacg gtaaatatcg gaacgatggt gcgccagcca aagtaatctg
     1261 gggatggcac ttaaagccag tgtttgtccg gtcgcgcgat aaacttcgtt ttcaaaggta
     1321 ttgttgtgca gttctttaag ttcgctaact tcgcgtgccg ctctggcatc cacattggcg
     1381 caggcccaga tcggggctcc ttcattatta tataaaacaa tgccttcacg catcgaacat
     1441 gccgaaacgg cagcgatata ctccggggct atgccggcgt tgtgcagcgc ctggcgcata
     1501 cactcacacg ccagttgcca gtttttgttg agatcaaatt ccatagaacc aggaacgtcc
     1561 ggtactgcca ggtgccgcca ctccgcctgt cccactgcta tttgattgcc ttccaggtcg
     1621 aataccacag cccgaatact tccggtgcct gcatccagcg ccatcaggta gtactttgat
     1681 tctgaagggg taaagagtcg agccattaga ttatcctcgg cttatgcgtt ctgtctgttc
     1741 ctctatacgt tctccatcat tcccggtaat aaggtcatgc aaatttaact acgtaaaatc
     1801 gccactgctg tgtcctgatc ggtaaccagt gcgttgatat aaccgccttt cattgcagcg
     1861 gcaattgctt cggctttatt ttctccccct gccacgccaa cccggacggg tatggtcttc
     1921 agcgcgctta aaggtaagcc aatcagttcg ttatgtattt tgatatccgg gacaacgtca
     1981 ccttttgcat caaaaaagta gcctaaaatg tcgccaaccg ccccttttcg gccaatcatt
     2041 aactgttcgc cctggctgat ataaccggag cgaatgatcg tcgcgtcgtc ttgttgactc
     2101 acggctccaa tgccgacaat cgccacatcg gctgcttgcg cggctaacag aacatctttg
     2161 acgcaatttt cattttttag cgtacgggca atgtcagcgg aggatgcccg caacggagcc
     2221 ggaataatat tcacactgca cgccgcgtta agctgcccga tacccgtcat ataagaaccg
     2281 acgccaccgg agagcgtgac caggcgaatt tgctgtgacg aaataaaacc acttaagcgt
     2341 tgcagcgtat tcatggttgc ctcgccaaaa ccaatcgcca gcatctgttg tggttgaagt
     2401 aaactcatca acatatgcgc cgcacctatc cctagtcgcc cgccgacatc agcatccgca
     2461 agcccaggga tcacccggac atgttgcagc gaaaactgac gacgtaattg agtttcatat
     2521 tccagacagc cttcaaagcg agaattaatc tgtacgcgaa taatgccgga ctgatgccct
     2581 ttctccagca atcgcgatac tttcaaacgg gtcaggccga gacgatcgct gatctcactc
     2641 tgggtcagcc cgtcgtggta gtaaaaccac gcgatccgcg cgacctgttc ttcttcacac
     2701 attccctgtt ctgaaattgc cgaatcgttg attgtcataa ttcattcttc actttgaaca
     2761 tatttaaatc tttaatgcaa ttgttcagtt cttgcgcatt tatatctgtg atggcaacca
     2821 cagtttgact ctacgagcat gaacaaacgc aaccgtgaaa atcaaaatag cataaattgt
     2881 gatctattcg tcggaaatat gtgcaatgcc cacctaaggt tatgaacaaa ttaaaagcag
     2941 aaatacattt gttcaaaact cacctgcaaa actgaacggg ggaaatatgc aaacgagtga
     3001 tacccgcgcg ttaccgctac tttgcgcccg ctcggtttat aaacagtatt caggggtcaa
     3061 tgtcctgaaa ggcatcgatt ttacgttgca tcagggggag gtccacgccc tgctcggcgg
     3121 caatggtgcc ggtaaatcga cgttaatgaa gattattgcc ggtattaccc ctgctgatag
     3181 cggtacgctg gagattgggg gcaacaacta cgccagatta acgccagttc atgctcatca
     3241 gctgggcatt tatctcgttc cccaggaacc gctgcttttc ccaagcctgt cgataaaaga
     3301 aaacatcctg tttgggatgg caaaaaaaca gctctccatg caaaaaatga agaacttgct
     3361 ggcggcgctg ggctgccagt ttgatctgca tagtctggca ggatcgctgg atgtcgccga
     3421 tcgccaaatg gtggaaatcc tccgcgggct gatgcgcgac tcgcggattc tgatcctcga
     3481 tgaacctacc gcctcgctta cccctgcgga aaccgaacgc ttgtttagtc gcttgcaaga
     3541 gctgcttgct actggcgtgg gtattgtttt tatctcgcat aagctgccgg aaattcgcca
     3601 gattgccgat cgaattagcg tgatgcgcga cggaaccatc gccttaagcg gcaaaactag
     3661 cgaactgtct accgacgaca ttattcaggc catcacccca gcggtacggg aaaaatcgct
     3721 ctctgccagc caaaaattat ggctggagtt acctggtaac cgcccacaac atgctgccgg
     3781 aacgccggtg ctgacactgg aaaatctgac cggcgaaggt ttcaggaatg tcagcatgac
     3841 gctcaatgcc ggagaaattc tgggcctggc tgggctggtg ggggccggac gcacagaact
     3901 ggccgagacg ctctatggtc tgcgtacttt gcgtggcgga cgtattatgc tgaatggtaa
     3961 agagatcaat aaattatcca ctggagaacg tttactgcgc ggtctggttt atctgccgga
     4021 agatcgccag tcatccggac tgaatctcga tgcttcgctg gcctggaacg tctgcgccct
     4081 tactcataac cttcgtggat tctgggcgaa aaccgcgaaa gataatgcca ccctggaacg
     4141 ttatcgtcgg gcgctgaata ttaaatttaa ccaaccggaa caagctgcac ggacattatc
     4201 cggtggcaac cagcaaaaaa tcctcattgc caaatgcctg gaagcttcgc cgcaagtatt
     4261 gattgtcgat gagccgacgc gcggcgtgga tgtctcggcc cgtaatgata tctaccagct
     4321 gttgcgcagc atcgccgcac agaatgtggc tgtgctgctt atctcctttg atctggaaga
     4381 gatcgaactg atgacagatc gtgtgtatgt gatgcatcag ggcgaaatta cccactctgc
     4441 actgaccggg cgcgatatta atgtcgagac tattatgcgc gttgccttcg gcgatagtca
     4501 gcgtcaggag gcatcatgct gaagtttatt cagaacaacc gtgaaatcac ggcattgctg
     4561 gcggtggtgc tgctgtttgt attacccggt tttctcgacc gtcagtattt aagtgtgcaa
     4621 acgctgacca tggtttatag cagcgcgcaa atcctgatcc tgctggcaat gggcgcgaca
     4681 ctggtaatgc ttacgcgcaa tattgatgtt tcagtgggtt cgattaccgg aatgtgcgcg
     4741 gtgctgttgg ggatgttact gaacgcagga tattcactac ctgttgcctg tgtcgcgact
     4801 ttactgcttg gtttgctcgc gggatttttc aacggtgtcc tggtcgcgtg gctaaagatc
     4861 cctgccattg ttgccaccct tggcacgtta gggttgtaca gaggcatcat gttgctgtgg
     4921 actggcggta aatggattga agggttaccc gccgaactga aacagctctc cgccccgctg
     4981 ctgcttggca tttcagcaat tggttggttg acgataattc tggtagcatt tatggcctgg
     5041 ctgctggcga agacggcgtt tggacgcagt ttttatgcca cgggcgataa tttacagggc
     5101 gctcgtcaac tgggcgttcg tacggaagcc attcgcattg tggcattttc gttgaacggc
     5161 tgcatggcgg cactggcggg aattgtgttt gcttcgcaga ttggttttat ccccaaccag
     5221 accggtaccg ggctggagat gaaagcaatt gcagcctgcg tgctgggcgg cattagtttg
     5281 ctcggcggtt ccggtgcgat catcggtgcg gtactcggcg catggttcct gacgcagatc
     5341 gatagcgtac tggtgctgtt gcgcattccg gcatggtgga acgattttat cgcgggcctc
     5401 gtgctgctgg cggtgctggt gtttgatgga cgcctgcgtt gtgcgctgga acgtaatcta
     5461 cggcggcaaa aatatgcccg ctttatgacg ccaccgccat ccgttaaacc cgcttcgtca
     5521 ggtaaaaaac gggaggccgc ataatgcgta ttcgctacgg ttgggaactg gctcttgccg
     5581 cactgctcgt tattgagatt gtcgcatttg gtgcaattaa cccgcgaatg ttagatctca
     5641 atatgttgct gttcagcacc agtgacttta tctgcattgg cattgtcgcc ctaccgctga
     5701 cgatggtgat tgtcagtggc gggatcgata tttcgtttgg ttcgaccatc ggcctctgcg
     5761 ccattgcatt gggcgtactg tttcaaagtg gtgtgccgat gccgctggcg atactcctga
     5821 ccttactgct cggcgcattg tgcgggctga tcaacgccgg attaattatc tataccaaag
     5881 ttaacccgct ggtgattacg cttggcacgc tgtatctgtt tgccggaagc gctctgctgc
     5941 tttccggtat ggccggagcg acggggtacg aaggtattgg tggattcccg atggcgttta
     6001 cagatttcgc taacctggat gtgctgggac tccccgttcc gctgattatc ttcctgatat
     6061 gtctcctcgt tttctggctc tggctgcata aaacccatgc cggacgtaat gtgtttttga
     6121 ttgggcaaag cccgcgcgtg gcgctttata gcgcgattcc agttaaccgc accttatgtg
     6181 cgctctatgc catgacgggg ctggcgtctg cggtcgccgc tgtgctgctg gtatcgtatt
     6241 ttggttcagc acgttccgat ctcggtgcgt cgtttctgat gcccgccatc accgccgtgg
     6301 tgcttggcgg ggccaatatt tatggtggtt ccggttccat tatcggcacc gccattgcgg
     6361 ttttattagt gggatatttg caacaaggtt tgcaaatggc aggagtgcca aatcaggtgt
     6421 ccagcgccct ttccggtgcg ctacttatcg tcgttgtcgt aggtcgttcc gttagcctgc
     6481 atcgccagca aattaaagag tggctggcgc gtcgggccaa taacccattg ccataaagga
     6541 tatcttcatg acacttcatc gctttaagaa aatcgcctta cttagcgctc ttggcattgc
     6601 cgcaatctct atgaatgtgc aggccgcaga gcgtattgca tttattccca aactggttgg
     6661 cgtgggattt tttaccagcg gtggcaacgg cgcacaacaa gcgggtaaag agctgggcgt
     6721 tgatgtgacc tacgacgggc cgacagaacc cagtgttcct ggtcaggtac agttgattaa
     6781 taacttcgtc aatcaaggtt ataacgccat tatcgtttct gcggtttcgc ctgatggctt
     6841 gtgtccggca ctgaaacgcg ccatgcaacg tggtgtgaga gtgctgacct gggactctga
     6901 tactaaaccg gagtgccgct cttactacat taatcaggga acgcccgccc agttgggagg
     6961 tatgttggtg gatatggcgg cgcgtcaggt gaataaagac aaagccaaag tcgcgttttt
     7021 ctactcaagc cccaccgtta cggaccaaaa ccagtgggtg aaagaagcga aagcgaaaat
     7081 cgccaaagag catcctggct gggaaattgt cactacgcag tttggctata acgatgccac
     7141 taaatcatta caaaccgcag aaggaatatt aaaagcgtat agcgatctcg acgccattat
     7201 cgcccccgat gccaatgccc tgcccgctgc cgcacaagcc gcagaaaacc tgaaaaatga
     7261 caaagtagcg attgtcggat tcagtacgcc aaatgtgatg cgcccgtatg tagagcgcgg
     7321 cacggtgaaa gaatttggac tgtgggatgt ggttcagcaa ggcaaaattt cagtgtatgt
     7381 cgcggatgca ttattgaaaa aaggatcaat gaaaacgggc gacaagctgg atatccaggg
     7441 cgtaggtcag gttgaagtct cgccaaacag cgttcagggc tatgactacg aagcggatgg
     7501 taatggcatc gtactgttac cggagcgcgt gatattcaac aaagagaata tcggcaaata
     7561 cgatttctga tgtgcattac ttaaccggag taagttatgg cagatttaga cgatattaaa
     7621 gatggtaaag attttcgtac cgatcaaccg caacaaaata tcccttttac cctgaaaggt
     7681 tgcggtgcgc tggattgggg aatgcagtca cgcttatcgc ggatatttaa tccgaaaacg
     7741 ggtaataccg tgatgctggc ctttgatcat ggttattttc aggggccgac taccggactt
     7801 gaacgcattg atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg
     7861 cgcggcattt tgcgcagcgt agttccccca gcgaccaata agccggtggt actgcgggcg
     7921 tcaggtgcga actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat
     7981 gatgccgtgc gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat
     8041 gaacatcagt cgatcaaaaa tattattcag ctggttgatg ccggaatgaa agtgggaatg
     8101 ccgaccatgg ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg
     8161 ctcgcgactc gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa
     8221 aaaggttttg aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa
     8281 aaattaccgg agcgcgaggc gctggaaatg tgctggcagg ctatcgatca gggcgcttct
     8341 ggtgtggata tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc
     8401 gtacaggcgg tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt
     8461 gaaaaacagt aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta
     8521 acgttcatga agacaagatt gacgagttta tcgaagtttt tcgccagaac cacctgggct
     8581 ctgtacagga agaaggcaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc
     8641 gcttttatat ttacgaagcc tataaagatg aagacgcagt ggcattccat aaaaccacgc
     8701 ctcactacaa aacctgtgtc gcgaaactgg aatctttaat gaccggaccg cgtaaaaaac
     8761 gtctgttcaa tggtttgatg ccgtgaggcg aatttatcaa ttttatctac aattggggta
     8821 acgcgctgac gggagtaaat aaatgtctga ctggaacccc tctttatatc tgcacttttc
     8881 cgctgaacga tcgcgtccgg cggtggagct gcttgccaga gtgccgctgg agaatatcaa
     8941 atacgttgcc gatcttggct gtggcccagg taacagcacc gccctgctgc atcaacgttg
     9001 gcctgcggcc aggataacag gcatcgactc gtctccggcg atgattgctg aagcgcgcag
     9061 tgctttgcga gactgccagt tcgtggaagc agacatccgc aactggcaac cggaacaggc
     9121 actcgatctg atttttgcta atgcctcact gcaatggctg cccgaccact acgaattgtt
     9181 tcctcacttg gtttctttac ttaatccgca gggcgtgctg gcagtacaga tgccagataa
     9241 ctggctggag ccgacccatg tgctcatgcg cgaagttgcc tgggaacaaa actacccgga
     9301 tcgcgggcgt gagccgttgg ctggcgttca tgcttactac gatattttga gcgaagccgg
     9361 atgtgaggtc gatatctggc gaaccaccta ctatcaccag atgccgtcgc accaggcgat
     9421 tatcgattgg gtgactgcca ctggattacg tccgtggtta caggatctga ccgagagcga
     9481 acagcagctt tttcttaagc actaccatca gatgctggaa gagcagtatc cactgcaaga
     9541 gaacggacag atactgctgg catttccgcg tctttttatt gttgcccggc gtatggagta
     9601 aatatcacgt cagctggtaa tgacgatcgg gaagaatttt tgctggaatt tcggcttcat
     9661 cgttcatctg taacaggtca atttcaatag cgttgcagat ggcatccagt ggtaaatcat
     9721 tgttttcagt accaaacgga tcttccagtt cttccgccag acaatccagc gaaataaaag
     9781 tgtaggaaat cagcacagag ataaaaggcg tcatgtaatg caggtccacg accagcgcga
     9841 acggcagcat gatacaaaac agataaacgg tacgatgcaa aatcagggtg taggcaaagg
     9901 gaattggcgt ataggcaata cgctcgcatc ctgccaggac cgctgatata tcattaagcc
     9961 gatcgttgag gctaataaac aggatatctg aaagctgtcc attgcggcgc tgaaccgcca
    10021 accattctcc cattattaac aagatacggt tagctggaga gttcgaagcc agtacacgct
    10081 gaagatcttc agtcttgaga taatgagcca gcacttccgc ctgtggctgt ttgcgtaatg
    10141 tcatgcgtaa acagtgggcg aaggcgattt gcagccgggc aaactccctt acacttgccg
    10201 aatccggcaa tgtcgttttt acctcgcgca gtaacgaccg tgaggcaatc atcaactgcc
    10261 cccaaagttt tcgcgcttca acgtaacggg cgtacccggc attattacga aaaccaagaa
    10321 aaatggcgat ggcgacaccg agaatgctga acggtgcgag ggtgaatttg atgcccagat
    10381 gcgtgtacca gggcagcatg aatataacag cgatagaaaa gagaaaattg agtagtaagc
    10441 gcgaggatat cttggataat actgagccgt gccagacaaa aatacggcgc agccagtgtt
    10501 gttgtggacg aacaatcatg gttatcttca ggcgtggaaa agtcgcccta ttaaacgtga
    10561 ttacgatcac attctcaaga cgctacttac aaattaccta caagcttaca acattaacag
    10621 gcgctgcatg tggcacctgt taatgataaa ggcacatagg atgttgtaac taatatagtg
    10681 aaataagaaa cccggtcaaa accgggttca gaagtaacgg tgttattagc acaacggacg
    10741 tacagcttcg cgcatccctt tttcaagaat cgcatccagg tcattagcaa cctgctcgac
    10801 cagaccaggc acttgcgtca ggtcctgctc ccagtggtct ttttctgcca aaacaatagc
    10861 taccagttcc tgggtgccga taacacggtc acgatgctgg ctccagagtt gctgataacg
    10921 ttccagccag tgtgcatcgt cctgtaccgg atatgtttct ccgttacgct caccgcgata
    10981 gaaggcaatc aatgccgcta atgcgaaagt aaggcgcgcc ggaagtgtgc cttttgcctt
    11041 ctgccctgcc agcagctgcg gcaggatgcg ggtgcggaac ttggtcatac cgttgagcgc
    11101 gatagacagc agctgatgct taatgtacgg gttacggaaa cgcccggtga ctgcactggc
    11161 gaaagattcc aattcatcac gaggcaaatc cagtaccgga ataatttctt cgtagatagc
    11221 tttttcaacg aatgcgcaaa tttcagcatc gttcatcgct tcacctacgg tatccagccc
    11281 tgcctggaac gccaccggca ccagcgcggt gtgagcaccg ttgaggattg ccactttgcg
    11341 ctctttatac ggtttaatat cgtcaacaat cagcacgttg agtggatatt tgtccaggcg
    11401 cagctcagtc gctaaggatt tcggcccctg aatcacaaac aggtaaaagt gttcagcggt
    11461 gtcgaggaaa ccatcgtgat aacccagctc ttcttccagt ttagccactt catcgcgcgg
    11521 ataaccggta acgatacggt ccaccagcgt agaacagaag ctgttagcct gatccagcca
    11581 ttgaataaat gcttctggca gtgcccactc ttgcgcatag cgcagcacca gttcacgcaa
    11641 cgcgtcgcca ttgtagtcga tcaactcaca cgggatgatg atccaacctt tatccagcgc
    11701 accgttgaaa tggctaaaac attcgaacag cagacgggtc agttttgccg gataacttac
    11761 cgctggcgcg tcatcgaatt tatcgcccgc atggtagctg atacccgctt cggtggtatt
    11821 agagaaaaca aagcgcattt ccgggttgtg agccagtttc aggaattcat cgtattcact
    11881 gtagacgctg atttcacgat taaccgagcg aatcagacgc gcgtcgctga ccgcttcccc
    11941 cttctcattc aggccacgga taatggtggt atacagacca tcctgcgtgc tcagtgacgg
    12001 tgggaatgaa gtttcaatcg gacgaacaac gaccacacca gaattcagat cggtgtgctc
    12061 attcaggaga tcgatttgcc agtcaacaaa ggcgcgcagg aagttacctt caccaaactg
    12121 aatgatacgt tctggatact gtgcaccggg aaaatcgcga cgatttagtg ttttcacaat
    12181 gggttccctt ctgattagtc atacaacctg tttgaattgg tacgacaggt tagcaaactt
    12241 taatacgccg aacccctgtt ttgatcaact cctgatgatt aatgagcagt tttatgagaa
    12301 aagtgtggcg cggatcatgg tttaatcgag gaaatacgcc ttttcctgga tcataaagtg
    12361 gtagaacaca ttgcattcaa atcgcgcgga atgaataaag atgtcagaca acttcctcac
    12421 cgtaacgcat agagctggta cggttgcgcc catctttctt cgagcgatac agacacgtat
    12481 cggcttcgac catcaattta ttaaaatcat cggtcagcat tcggtacgat gcgcgaccac
    12541 taccgacgcc aatacttacc gtgagataaa gcgttttttg ttgccaggtg aatggttgca
    12601 gttcaacgcc tttacggatt ttttccgcca ttagcagacc atcgacagga ttcaccgacg
    12661 gcactgcaac agcaaattct tcgccgccca tgcgcgccac cagcccctta tcaccgacaa
    12721 tcttctgaat atgccgggca aacacgctta acactttatc gccacattca tgtccatagt
    12781 tatcgttaat gcttttgaaa tagtcgatat caagcagcat gaccgtcaga tgttgtgtct
    12841 gtttcagcga tggacttttc agcgcctcat aaagaccgga ccgtgagtaa acctgagtca
    12901 gaaaatcaaa gtcggctcgc agcgcaactt gcttcattag cgaattgatc gctgccacgc
    12961 taaaagaaac cataattggg catatcgcca tcgtggcaat accgagacgt gcggagaaca
    13021 tttccggaat ggagaacggc gaaccgaccg agatatcaat caccgaattt gccaccagta
    13081 cgatttccac cgcaccggtg acaaaggtca acagacatgt tacctgcggc gtatagcgca
    13141 ctgcacacca gattaatgca ggcagcggga acgccagact ccccgctccg ccaatgacta
    13201 ccgaggcaat aaccgacaca ataagcgcga tggcgggcat catctgttct gctttaaagc
    13261 gcggcagcac tccaggaata gccaacgtca gcatacaagg cacgatcaac acgcccgttg
    13321 agaattgctc actgaaccag tccgcaagca aaggccagaa atccagacta tcaatactga
    13381 ccgaaccaat cgccccgaca atagcgcata ataatgcggc aatgagacag taattaaata
    13441 gccgtaaggc acttactggc tcatacttat ttttccccag acgcttatcc cgcacgacca
    13501 gtaaggcgac ggtaacaata aacatcatat tggagaaatt gataaccagt gaaaccagtc
    13561 cccattcggt ggttatggca tcataaacca gcatcgccac ataactgatc gcatagtaat
    13621 gcagtcgatt aagccagaca tagcgggcaa acacccccgc cattacgcca ttcagcggcc
    13681 agaaaagaga aagttcgtga accagccgta actctgcacc aataaaatag aacagcgtgg
    13741 ttagcgcaaa aatagcgatg ctattacgta gcagatgacc ttcctggaac aaacggaaag
    13801 ttgaaatggg gtgtacatgc attaattatc tgtccataca attgcgggtg gcaattatct
    13861 caacgtaata agtaaattct cataaaggat agcatgttcg ctaacagcgg cggcaaataa
    13921 aggtaccctg ggaatgctat tttatcgttt tccgcaattc tcatggctga ttcatctgta
    13981 caaccagcca gtgatagatt accgccacga tataacgttg ttgttcttcg cttaacgaaa
    14041 caccctgcgg aatgttatgc cacttagcca gacaaccgcg acagcaggtc gcggtggcgt
    14101 gctgagcgat aaacaccgga tgcccgcgca tgggggtttg cttaccgtca ttagccggta
    14161 atgccggagc aagacgttta gcgacaaaat ccgccgcatg ttgttcgatg actggcgcgc
    14221 ctttctccag gcaatactga cgctctttca tgcccagacg aaagcgagag cgaaattttg
    14281 aacgggataa acgcgcgaag agtggatcga gagactgcat taataaaccg aacgccctaa
    14341 ctgtttcgtc aattgttcaa ggacggcaat acccgcaagc gagttacctg catcatccag
    14401 ttccggactc cagacagcga tggccatttc atgcggaaca atcgccacaa taccgccacc
    14461 aacgccagat ttcgccggta gccccacccg ccaggcaaac tcccccgcgt tctggtacat
    14521 accactggtc gccatcagcg cgttaatttg ccgcgcctgc attggcgtca ccacaggttc
    14581 atcaatatga atagctttcc cctgattagc cagaaagaca aacgtccggg ccagctctac
    14641 acagctcatt ttcagagcgc agtaatgaaa gtagttttgc agaacggttg tcacatcatg
    14701 atggaaattg ccaaacgact tcatcagcca ggcgatagcc gcatttcgcg cggaatgttc
    14761 aaattcggaa cgcgctacca ccgtatcgta tgaaatatca gacacaccgc ttaagccgcg
    14821 cacgacttcc agcatacgtt gccgtggtgc gcttaatcgc ccttgcaaca tatcgcaaac
    14881 caccagcgca ccagcattaa taaacggatt acgcggaata ccctgctcca tttccagttg
    14941 cactaaagaa ttgaacggtg atccagacgg atctttgccg acgcgttgcc agatttcctc
    15001 ttcggagtaa tgacgcatgg cgacaacgag actcagcact ttggaaatag actgaatgga
    15061 aaaacgttct tgcgcgtctc cggcctgaaa aagctgtccg tcaacggtac agatagcaat
    15121 gcccaatcgg gaaccgtcta ctgtagccag cgccggaata taatccgcga ctttaccctg
    15181 accaatgagc ggccgcactt gccgcaagat gttttctaaa attgcattat ccatggcgac
    15241 tgccactttc tactcctgga ccgcaggtct gaaaagacct gcgagtatat cagagctgaa
    15301 tatgtcgcgt cagatccggt ctttccacac cgtctggata ttacagaatt cgtgtaagcc
    15361 gaaatgggaa agctcacgac caaagccact ctttttcacg ccaccaaagg ccactcgcgc
    15421 gtcgctggca caataaccat tgataaacac cccaccgcat tccagacgtg ccgccatctg
    15481 tctggcctgt gtttcgtcgg tggtaaaaat ggtcgctgaa aggccgaact cactatcatt
    15541 agccagttcc agcgcatgtt ctgcatcttt cgcaacggtg attgccgcaa cagggccaaa
    15601 catttcttcc cgaaacgcgg tcatttctgg ggtaacattc gccagaaccg ttggcggata
    15661 gtagttacct gccccagcca tcttttcccc gcccagtaac aaacgcgcac cctgcgccag
    15721 ggttttctcc acctgatgat gcagctcatc acgtaaatca aaacgagcca ttggtccgag
    15781 agcgttctct tcatcacggg gatcgcccat tttcaaggct gccgcagctg ccacaaaacg
    15841 ttcggtaaat gcagaagcaa ttccctcttc gataataaag cgttttgccg ctgcacaaac
    15901 ctgtccggta ttctgataac gtccggctac cgccgcttta accgccagtt ccagatcggc
    15961 atcgttaagc acaataaatg gatccgaacc gcccagttcc agtacgcatt ttttcagtgc
    16021 cgctccagcc tgtgcgccaa tagccgctcc cgcacgaaca cttccggtca ccgtgacagc
    16081 agcaatgcgc gaatctttaa tcatttgact gacaccgtcg ttgtcggcat tcagccagcc
    16141 atatacgcct tgcgggattc ccgcatcttt aaacacctgg gcaatgagct gagcacagcc
    16201 catcacattc ggcgcatgtt taagtaagta gccgttacct gcaagaatga tgggaaccgc
    16261 gccacgcatc acctgccata acggaaagtt ccacggcata atcgccagaa tcgtccccaa
    16321 cggtcgatac tcaataactg cctgctgatt ttccaccagc gtaggttccg ccttcagcat
    16381 tgccggacca tgttctgcat accagtcaca caaattcgcc gatttcgcca cttcagcgcg
    16441 cgcctgattg attggtttgc ccatttcacg ggtgatcatt tgcgccattt cttcgctacg
    16501 ggcgcgcaga gccttaccga taccacgcag tttttcagca cgatgatcta tatttgtctc
    16561 gcgccagtcg cgaaagcctg ctgccgccag ctgaagtgcg ttttcgatat cgttagcgcc
    16621 agcccacggc agcacagaaa gttgttcacc cgtggcagga tttattgaaa ttgcatgagt
    16681 tgccggagta atggtcatcg gggtatctcc tttatgagtc atggtatgaa gatacgcaga
    16741 tttactcttg ctttaaaatg aataatatta agccacttat tcacgaattc aacgcggttg
    16801 ccgaagctgg cagcattacc caggctgcag caaaagtgca tcgcgtaccg tcgaatctca
    16861 ctacccgtct acgccagctg gaaacagaac tgggggtaga tctgtttatt cgcgagaatc
    16921 agcgtttacg tctctctcct gccgggcata actttttacg ctatagccaa caaattctcg
    16981 cgttagtgga tgaagcgcgg agcgttgtcg ctggcgatga gccgcaaggt ttattttctc
    17041 ttggttcgct ggaaagcacc gcagcagtgc gcattccggc cacgctggcg gaattcaacc
    17101 atcgttatcc caaaattcag ttttcacttt ccaccggccc ttccggcact atgctggaag
    17161 gagtgctgga gggaaaactg aatgcggcat ttattgatgg gcccattaac catactgcca
    17221 ttgacgggat accggtatac cgcgaggagc tgatgatcgt cacgccacaa ggtcatgcgc
    17281 cagtaatccg cgccagtcag gttaacggca gtaacattta tgccttccgc gccaattgtt
    17341 cgtatcgtcg ccacttcgag agctggtttc atgctgacgg tgccgctccg ggaactattc
    17401 acgagatgga gtcgtatcac gggatgttgg cctgtgtggt cgcaggagca ggcattgcgc
    17461 ttattccacg ctctatgctg gaaagtatgc cggggcatca ccaggttgaa gcgtggccgt
    17521 tagctgagca atggcgttgg ttaacaacct ggctggtctg gcgtcgcggt gcgaaaacgc
    17581 gtccgcttga ggcatttatt caactactgg atgtgcctga ctcggcaaaa cagggttacc
    17641 aatgagctat ttttgatagt tcttgcagct caaatgccct atatagtgat gttccgatga
    17701 cttatgacta tatggggcaa atatggttac gccagtaagc atcagtaatt acatatctct
    17761 tcctgatgat tttcccgtac gtaatattgc acctcaggta aaagaagttt taaaggactt
    17821 tattgatgcg ctaagtacaa taatatgtga tgaagagtgg cgcacgtctt taaacatcaa
    17881 ctcagccaca aaaaagatat ttaataacct tgataactta tcatatattc agaggacttc
    17941 ttttcggggt aacgacacgc tatacaatga aaaggttcag tttaaactca cttatcccgt
    18001 caagaatgga agacacaaag aaaatattga atttcaggta gtaataaatt taagccctat
    18061 ttatttagat aattttcgcc atgatggaga aattaatatt tttgcgctcc caacccaaag
    18121 cctgtcacta tggggcgcgt atttcagacc ggcgtcgagc gtgtgctatt tctgtttctg
    18181 aatgatttta ttgaacaatt tccaatgatc aaccttggcg ttcccatcaa aagagcgcat
    18241 acaccacata ttgaacccct gcctccggat cgccataccg ctgcagatta cttacgccag
    18301 tttgatttgc ttgtcctgaa ttttatctct cgcggtaatt ttgtcatact cccccgatta
    18361 tggagtaact ctgaggttca cagatggttc gtcaataaag atcctaactt gatcaccgcc
    18421 attctcgaca taacagacgg cgaattaaaa gaggatttat tgcaaagcct gatggattca
    18481 ttaggttcta acaaacatgt actacccgaa gtctgcatct gctttttatc ccttttagca
    18541 gaacaagaat ctcctcattt tcaggacttt ttttattttt cgccaatatg ttattgcact
    18601 atcaccaatt tatgaatccc aatgaaagtg atctgaatga cgtgttgatg ccagcatcat
    18661 taagtgatga taaaattatc aaacatatgg cgcgcaggac cctcaaactg tttgtaaaaa
    18721 atgaaacgcc gccaaaagtt actcacgaag acctggtgaa aaacaggcct cgctcccctg
    18781 tcagaccacc tatacccgca accgccaaaa cgccagacct ccctgaacgt cattaaaccg
    18841 tgatgttacc gactctctga cgcggaacag aatcagcgtc agagaaacgg aaaacgcgat
    18901 ccagatcaca aatgcattgt attcacatca ttaaccgttt taagatcatt tcatcacttt
    18961 ttcgcaactc acccgataat ctgttatgac aacaaacact gtttcccgca aagtggcgtg
    19021 gctacgggtc gttacgctgg cagtcgccgc cttcatcttc aacaccaccg aatttgtccc
    19081 tgttggcctg ctctctgaca ttgcgcaaag ttttcacatg caaaccgctc aggtcggcat
    19141 tatgttgacc atttatgcat gggtagtggc gctaatgtca ttgcctttta tgttaatgac
    19201 cagccaggtt gaacggcgca aattactgat ctgcctgttt gtggtgttta ttgccagcca
    19261 cgtactgtcg tttttatcgt ggagctttac cgttctggtg atcagtcgca ttggtgtggc
    19321 ttttgcacat gcgattttct ggtcgattac ggcgtctctg gcgatccgta tggctccggc
    19381 cgggaagcgg gcacaggcct tgagtttaat tgccaccggt acagcactgg cgatggtctt
    19441 aggtttacct ctcgggcgca ttgtgggcca gtatttcggt tggcgaatga ccttcttcgc
    19501 gattggtatt ggggcgctta tcaccctttt gtgcctgatt aagttacttc ccttactgcc
    19561 cagtgagcat tccggttcat tgaaaagcct cccgctattg ttccgccgcc cggcattgat
    19621 gagcatttat ttgttaactg tggtggttgt caccgcccat tacacggcat acagctatat
    19681 cgagcctttt gtgcaaaaca ttgcgggatt cagcgccaac tttgccacgg cattactgtt
    19741 attactcggt ggtgcgggca ttattggcag cgtgattttc ggtaaactgg gtaatcagta
    19801 tgcgtctgcg ttggtgagta cggcgattgc gctgttgctg gtgtgtctgg cactgctgct
    19861 acctgcggcg aacagtgaaa tacacctcgg ggtgctgagt attttctggg ggatcgcgat
    19921 gatgatcatc gggcttggta tgcaggttaa agtgctggcg ctggcaccag atgctaccga
    19981 cgtcgcgatg gcgctattct ccggcatatt taatattgga atcggggcgg gtgcgttggt
    20041 aggtaatcag gtgagtttgc actggtcaat gtcgatgatt ggttatgtgg gcgcggtgcc
    20101 tgcttttgcc gcgttaattt ggtcaatcat tatatttcgc cgctggccag tgacactcga
    20161 agaacagacg caatagttga aaggcccatt cgggcctttt ttaatggtac gttttaatga
    20221 tttccaggat gccgttaata ataaactgca cacccataca taccagcagg aatcccatca
    20281 gacgggagat cgcttcaatg ccacccttgc ccaccagccg cataattgcg ccggagctgc
    20341 gtaggcttcc ccacaaaata accgccacca ggaaaaagat cagcggcggc gcaaccatca
    20401 gtacccaatc agcgaaggtt gaactctgac gcactgtgga cgccgagcta ataatcatcg
    20461 ctatggttcc cggaccagcg gtacttggca ttgctagcgg cacaaaggca atattggcac
    20521 tgggttcatc ttccagctct tccgacttgc ttttcgcctc cggtgaatca atcgctttct
    20581 gttgcggaaa gagcatccga aaaccgataa acgcgacgat taagcctcct gcaattcgca
    20641 gaccgggaat cgaaatgcca aatgtatcca tcaccagttg cccggcgtaa tacgccacca
    20701 tcatgatggc aaatacgtac accgaggcca tcaacgactg acgattacgt tcggcactgt
    20761 tcatgttgcc tgccaggcca agaaataacg cgacagttgt taatgggtta gctaacggca
    20821 gtaacaccac cagccccagg ccaattgctt taaacaaatc taacattggt ggttgttatc
    20881 ctgtgtatct gggttatcag cgaaaagtat aaggggtaaa caaggataaa gtgtcactct
    20941 ttagctaacc ttgcatcgca ttgaccaaaa cttgaaccga tttatcaaaa cgtggcatcg
    21001 gtcaattcat ttatttgact tatacttgcc tgggcaatat tatcccctgc aactaattac
    21061 ttgccagggc aactaatgtg aaaagtacca gcgatctgtt caatgaaatt attccattgg
    21121 gtcgcttaat ccatatggtt aatcagaaga aagatcgtct gcttaacgag tatctgtctc
    21181 cgctggatat taccgcggca cagtttaagg tgctctgctc tatccgctgc gcggcgtgta
    21241 ttactccggt tgaactgaaa aaagtgttgt cggtcgacct gggagcactc acccgtatgc
    21301 tggatcgcct ggtctgtaaa ggctgggttg aaaggttgcc gaacccgaat gataagcgcg
    21361 gcgtactggt aaaacttacc accagcggcg cggcaatatg cgaacaatgc catcaattag
    21421 ttggccagga cctgcatcaa gaattaacaa aaaacctgac ggcggacgaa gtggcaacac
    21481 ttgagcattt gcttaagaaa gtcctgccgt aaacaaaaaa gaggtatgac gatgtccaga
    21541 cgcaatactg acgctattac cattcatagc attttggact ggatcgagga caacctggaa
    21601 tcgccactgt cactggagaa agtgtcagag cgttcaggtt actctaaatg gcacctgcaa
    21661 cggatgttta aaaaagaaac cggtcattca ttaggccaat acatccgcag ccgtaagatg
    21721 acggaaatcg cgcaaaagct gaaggaaagt aacgagccga tactctatct ggcagaacga
    21781 tatggcttcg agtcgcaaca aactctgacc cgaaccttca aaaattactt tgatgttccg
    21841 ccgcataaat accggatgac caatatgcag ggcgaatcgc gctttttaca tccattaaat
    21901 cattacaaca gctagttgat aacgtgacaa cgtcactgag gcaatcatga aaccactttc
    21961 atccgcaata gcagctgcgc ttattctctt ttccgcgcag ggcgttgcgg aacaaaccac
    22021 gcagccagtt gttacttctt gtgccaatgt cgtggttgtt cccccatcgc aggaacaacc
    22081 accgtttgat ttaaatcaca tgggtactgg cagtgataag tcggatgcgc tcggcgtgcc
    22141 ctattataat caacacgcta tgtagttatt gtcaggctct gatcacagag cctgacatct
    22201 ccgtaatatc ttcctcaagt ctcaaatatt ttcgccattg ttggcgatga gatcccgata
    22261 ccagtaaaac gacgctttac gtatccgttc cagcgttccc ttcccttcgt tgtctttatc
    22321 gacataaatc attccgtagc gctttttcat ttcccctgtt ccggcagaaa ccaggtcaat
    22381 gcagccccac ggggtgtagc caattaagtc aacaccatct tcaactacgg cttttttcat
    22441 ttcccgaata tgggaagcaa agtaatcaat gcgatagtga tcgttcaccg tgccgtcagc
    22501 ttgtctctgg tcaaccgcac caaatccatt ttcgacaata aacagcggca actggaaatg
    22561 atcccagaac cagttgagtg aataacgtag ccctgccgga tcaatttgcc ccccccattc
    22621 ggatgtatcg atataagggt tgctgaccaa atctcgtggt tcaacataat ccagttgcgg
    22681 gttatcgggc gaaaattgtg tcgtaaaaga catgtagtag ctaaagccga taaagtcgac
    22741 acaaccactg gcaagaatcg cgttatcttc tgtgtgatat cgaggttgaa tcctttcctg
    22801 gcaaagtaat tcagcatatg ttgcggataa tatccacgag catgaacatc agtaaaccag
    22861 taacgacgat gcatcgcttt cgtggccatc atcatatcgt tgggtgcaca cgtcagagga
    22921 tagatggggc acatggcgat catacagcca atgttaaatt caggattgat cgattttcca
    22981 gtctgtaccg ccagggcact ggcaactaac tcgtaatgta ccgcctggta cattatttgt
    23041 tcgcgctcat tgatatcttc ctctggcgaa tacaagatac cggaattagt aaatggacac
    23101 aggctttcgc tgaaattcac ctgattattg atttcgttaa acgtcatcca gtactttact
    23161 ttttctttat agcgcgtgaa gaccgttgat gcgaagcgga taaaaaagtc gatcagttta
    23221 cggtttcgcc agccaccata ttttgtcacc agatgataag gcatctcaaa atgtgaaagc
    23281 gtcaccacag gttccattcc ctgcttaagg cattcatcga acagatcatc ataaaattgt
    23341 aaaccctctt cattcggctc ctgttcgtca ccttgcggaa agattcgtgt ccaggcaatg
    23401 gaagttcgaa agcatttgaa tcccatctcg gcaaataact gaatatctgt tttatagcga
    23461 tgataaaaat caattgcttc atgattggga taattaagcc cgtcgataac gccttctgtc
    23521 acttcacgcg gcaccccgtg agcgccagca gtcatcacat cagcgatgct gatgcctttt
    23581 cctccttcat tccagccacc ttccaactga tgcgcggcta ccgcgccgcc ccataaaaaa
    23641 ccttttttaa atcctgacat acacaactcc ttaactaaaa tagattacca ccagacttcc
    23701 gcctggatgc cggccataaa ctcgtctttg ctgttctcat taaacttaaa attcgataat
    23761 tcgttatcca gaatattgat atacgttcca tagaagcgaa tttctggtcg tgaacctaaa
    23821 atgctttcac ccactttcaa tgcatgccag agtgtcgttt tataagccga ttcatttagt
    23881 gttacaccct gctgagtttt gttctgttgc ttaaaccagc ctaattcaag ccccgtctga
    23941 ttccatgtat tccagatcca ggccggtctt attacagtgc gaatactgtc aaaatcacta
    24001 tgagcaccac tttcataact ataaacatct tcgccgtgag aatagacaag cgcgttagcc
    24061 ataataatat tgtcagtaag atacatctcg ccctgagaga ttaaacgcca ggcgatccca
    24121 ttggtatgat caccatagta atagcgacca tgcgccatcg tattactggc atctgagaaa
    24181 ctggcaaaac tgctggcata ggaattattg gcaacctgta acgtaaattc attaaacgta
    24241 tcgcgttgca ggttttgccg taaaacagaa gtcagcatcc agctatcttt catttcaaaa
    24301 taactgttgt cattttcgtt atcttgttgt tgatccgttt tattaggtgc ggaatattta
    24361 gccattaatg ataatgtcgc atcatcccat aacgggatat tgcgataacg aacatccaca
    24421 gaattggtat tcatctggct ggtacgcgaa aaatcacgag agtaaacatc gacatcatca
    24481 cggcttaagg acatatcaaa cagccctaca ccaagtgccc agttttcaat ccccacaccc
    24541 gcggcgacat ccgtggttaa ggttttccag tccagcatct ggatctcata ttgcgggagt
    24601 ttatgtttgc cgacccagaa gtctgcctct ggtgcgaagg gtaaaaaacc tcgcgttgtc
    24661 aggtaaatat cgctgaactg catgatattt tcattggcac tgtcaccaaa ccaggcatcg
    24721 ttatactgct cacccacgtt gccatcatag gtaacgaccg catttgccgt tttaccgtcc
    24781 tgattataaa cacgctgatt taaggtcagg tcaaaccaac cgctcatctc attaccaaaa
    24841 cgtcccagag aacccgctgc ataagtttgt ggtgagccgt gattaccagt accccagcct
    24901 gagcggaagt acccctgata gctgaaccca atatcatcct ttatatattt actgatatct
    24961 ttcagggtca cgctttcgat agtggttttg tcgcctttac tttcaataac ggcaacttgc
    25021 tgcgatccat ttatagtatg cgctgcggta aacgagttct gttcaccgtt ttcatttttg
    25081 atgttatcag cgacgggaga tgtcgctgat atttcagcaa gcgaggctga tttatatttg
    25141 ttttccgtga tgcttttttg taaggtcgaa agtcgggatt tatatactcc cagttcattc
    25201 tgtgttgctt tcagctcttg tttattttgc gacaattcat tttcaagcaa ttcaaggcgc
    25261 tgctctatgg ttaattttgt tgccatcgcg ttcaatggaa acaatgctgt aataatagaa
    25321 aaagacaaaa ggctgacgtt aagcgtcttt atattcatga cttatcctta agagtataat
    25381 aatttcctcc agctatcgca ctggaggaaa aaatcaggca tggagttctt ttcttaatga
    25441 aataatttcc ctggctaaat ccaggcataa tattgcattc atcaaatgat cctgtgcatg
    25501 acccagaatt aaattaactt ttattttgcc acagccttca tcctgactaa ttaatgcggt
    25561 ctgaatctta tgtgcctgaa gacaggcaga ttcagaactg gccattagct gagttgcgcc
    25621 ctgccagtct ttttgcccgg cacaacgtat ggcttccatc gcgtcagaac gtgcctgccc
    25681 cgcgttgacc agtaactcca taacaagttc ttcatctgca aacatcacaa tctccttcct
    25741 gggtaaatgg aattatccgt tagttgtcat gttattgact ttgttggcgg caataacaaa
    25801 aggtaaatag atcattgtcg ccacacctaa acataaaaaa ccaaccagta aagccagcca
    25861 gttccccccg gttccgagga aagcaattaa aggtccgggc gtagtccagg gcacggcata
    25921 agcaaccggt ggaataattt ccatcgaaac aaagaagtaa ccaatggcac agttaacaat
    25981 gggaaccata ataaacggca ccatcatgat ggggttaagt acaataggaa ggccaaacat
    26041 tatcggttca ttgatattga aaataccggg gataaatgac atttttgcca gatcacggta
    26101 ttccgcacgc ttagaagcga taaaaatagc caacaataac cccaacgtca tacctgaacc
    26161 gccgcagttg gcgaatgcat cattaataga agtccaggta atcggatatg gcgcgcccca
    26221 ggtagtgcca tgttgagcgg cccaggagag attctccaga ttagcctcag aaaaaacggt
    26281 ttctcgaatt gccgatgtgg tattaggtcc gtggatcccc agcacccaca ggaaattacc
    26341 cacagccccc aggataatta ccgcaaagat attcgttccc atatgtttga gcggcgtctg
    26401 gattaatgtg taaataagat cgtttagtcc tgccggggat atcagtgtca ggcaataatt
    26461 aagtgcggaa aagaacacca tgacaaaaaa tattggcaat aaaactttaa atgaacgcgc
    26521 taccgcaggc ggtacaactg ccggcatcgt tatggtgatt tttgggtttc gagctaagcg
    26581 acaaaatatt tcactggtga tcaatgcaac gataacagca acaaacaacc cctgcgcgcc
    26641 gagatatttg gtcgtcagga atgcttgccc atctatcatc tgatatggcg tataaacaat
    26701 aaaaaatgct cctattgccg ttaatccgca taaaagatca tcttgctcat agctaatcgc
    26761 catattcctg gcgaccagga aagcaatcat aattgacatg atatttacag aaccattcat
    26821 taccggagta aaaatagctt gtgctttttc aaggttgggg aaaatgctat tcagatgcag
    26881 taatccggca ataaaaccgt ctggcgataa tatggcaaag ttaattaata taattaatga
    26941 acttgccatg ataattggaa acgtaaaaac gaatccatct ctcaccgc
//
LOCUS       DAZKIV010000052        26951 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZKIV010000052 DAZKIV010000000
VERSION     DAZKIV010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.052"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..1661
                     /gene="rtcR"
                     /locus_tag="ACHOT6_002148"
     CDS             63..1661
                     /gene="rtcR"
                     /locus_tag="ACHOT6_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFP9947366.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHASLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(1643..2401)
                     /locus_tag="ACHOT6_002149"
     CDS             complement(1643..2401)
                     /locus_tag="ACHOT6_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFP9947367.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2418..3248)
                     /gene="glpG"
                     /locus_tag="ACHOT6_002150"
     CDS             complement(2418..3248)
                     /gene="glpG"
                     /locus_tag="ACHOT6_002150"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFP9947368.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLKARKRK
                     "
     gene            complement(3293..3619)
                     /gene="glpE"
                     /locus_tag="ACHOT6_002151"
     CDS             complement(3293..3619)
                     /gene="glpE"
                     /locus_tag="ACHOT6_002151"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFP9947369.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3809..5314
                     /gene="glpD"
                     /locus_tag="ACHOT6_002152"
     CDS             3809..5314
                     /gene="glpD"
                     /locus_tag="ACHOT6_002152"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFP9947370.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5368..5973)
                     /locus_tag="ACHOT6_002153"
     CDS             complement(5368..5973)
                     /locus_tag="ACHOT6_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947371.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(5976..6743)
                     /locus_tag="ACHOT6_002154"
     CDS             complement(5976..6743)
                     /locus_tag="ACHOT6_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947372.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYQVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6746..7456)
                     /locus_tag="ACHOT6_002155"
     CDS             complement(6746..7456)
                     /locus_tag="ACHOT6_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947373.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7472..8981)
                     /locus_tag="ACHOT6_002156"
                     /pseudo
     CDS             complement(7472..8981)
                     /locus_tag="ACHOT6_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
     gene            complement(9107..11554)
                     /gene="glgP"
                     /locus_tag="ACHOT6_002157"
     CDS             complement(9107..11554)
                     /gene="glgP"
                     /locus_tag="ACHOT6_002157"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFP9947374.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(11573..13006)
                     /gene="glgA"
                     /locus_tag="ACHOT6_002158"
     CDS             complement(11573..13006)
                     /gene="glgA"
                     /locus_tag="ACHOT6_002158"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFP9947375.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(13006..14301)
                     /gene="glgC"
                     /locus_tag="ACHOT6_002159"
     CDS             complement(13006..14301)
                     /gene="glgC"
                     /locus_tag="ACHOT6_002159"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFP9947376.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(14319..16292)
                     /gene="glgX"
                     /locus_tag="ACHOT6_002160"
     CDS             complement(14319..16292)
                     /gene="glgX"
                     /locus_tag="ACHOT6_002160"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFP9947377.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(16289..18475)
                     /gene="glgB"
                     /locus_tag="ACHOT6_002161"
     CDS             complement(16289..18475)
                     /gene="glgB"
                     /locus_tag="ACHOT6_002161"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFP9947378.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(18748..19851)
                     /gene="asd"
                     /locus_tag="ACHOT6_002162"
     CDS             complement(18748..19851)
                     /gene="asd"
                     /locus_tag="ACHOT6_002162"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFP9947379.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            20043..20636
                     /gene="yhgN"
                     /locus_tag="ACHOT6_002163"
     CDS             20043..20636
                     /gene="yhgN"
                     /locus_tag="ACHOT6_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFP9947380.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(20693..22033)
                     /gene="gntU"
                     /locus_tag="ACHOT6_002164"
     CDS             complement(20693..22033)
                     /gene="gntU"
                     /locus_tag="ACHOT6_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFP9947381.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(22037..22564)
                     /gene="gntK"
                     /locus_tag="ACHOT6_002165"
     CDS             complement(22037..22564)
                     /gene="gntK"
                     /locus_tag="ACHOT6_002165"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9947382.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLHDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(22703..23698)
                     /gene="gntR"
                     /locus_tag="ACHOT6_002166"
     CDS             complement(22703..23698)
                     /gene="gntR"
                     /locus_tag="ACHOT6_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFP9947383.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(23922..24617)
                     /gene="yhhW"
                     /locus_tag="ACHOT6_002167"
     CDS             complement(23922..24617)
                     /gene="yhhW"
                     /locus_tag="ACHOT6_002167"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFP9947384.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(24740..25777)
                     /gene="yhhX"
                     /locus_tag="ACHOT6_002168"
     CDS             complement(24740..25777)
                     /gene="yhhX"
                     /locus_tag="ACHOT6_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFP9947385.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEDAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            26110..26598
                     /gene="yhhY"
                     /locus_tag="ACHOT6_002169"
                     /pseudo
     CDS             26110..26598
                     /gene="yhhY"
                     /locus_tag="ACHOT6_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            26835..>26951
                     /locus_tag="ACHOT6_002170"
     CDS             26835..>26951
                     /locus_tag="ACHOT6_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP9947386.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEI"
BASE COUNT         6751 a         7188 c         6704 g         6308 t
ORIGIN      
        1 gagatttccc gcaaagccaa tttaccgata atgaaatatc gtcttttata aggatatcta
       61 agatgcgtaa aacagtggct tttggctttg tcggtaccgt actggattat gccgggcgcg
      121 gcagtcagcg ctggtcaaaa tggcgtccga cactctgttt atgccagcaa gaatcgttgg
      181 tcatcgatcg actggaattg ttgcacgacg cccgctcgcg ctcgctattt gaaacgctta
      241 aacgcgatat cgccagcgtt tcgccagaaa cagaagtggt gagcgttgag attgaactgc
      301 ataacccgtg ggatttcgaa gaggtctacg cctgcctgca tgatttcgcc cgtggttacg
      361 agtttcagcc agaaaaagaa gactatttaa ttcacatcac caccggtacc cacgtcgcgc
      421 agatttgctg gtttctgctg gcagaagcgc gttacctgcc cgcccggctg atacaatctt
      481 cacctccgcg caaaaaagaa cagccgcgcg gcccaggtga agtaacgatt atcgatctcg
      541 atttaagccg ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg
      601 attttcttaa gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga
      661 tcgaaaaagt ggcgatcaaa tcccgcgcgc cgattctgct taacggtcca accggcgcgg
      721 gcaagtcatt tctggcgcga cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg
      781 gcgcgtttgt ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt
      841 ttggtcatgt aaaaggcgcg tttaccgggg cgcgggaatc tcgtgaaggt ttattacgca
      901 gcgccaacgg cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacagg
      961 caatgctgct gaaagccatt gaagagaaaa ccttttaccc gtttggcagc gatcgccaag
     1021 tgagcagtga ttttcagctt atcgccggaa cggtgcgcga tttgcgccag ctggttgccg
     1081 aaggcaaatt tcgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg
     1141 gtctacgcca gcgccaggaa gatattgaac cgaacctgga ttatgaagtg gagcgccacg
     1201 cctcactcac tggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt
     1261 ttgcgacctc tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca
     1321 cgcggatggc cacctttgcc actagcggac gcatcactct ggacgtggtt gaagatgaga
     1381 taaaccgtct gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg
     1441 gcgcagaggc agaaaacatc gatctcttcg accgtatgca actggaacac gttatcgcta
     1501 tctgccgcca ggcaaagtcg ctttccgcag ccggacgcca gctttttgac gtttcgcgcc
     1561 agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc
     1621 tgacgtggga agccgtgcag gatcagcaca gctccagttg aatatggtgg tccgtcagca
     1681 cctgcatcac gcttactggc ggcggggcgt cggtgtagac ggcatctacc atgctgatgc
     1741 tgcccatatt gaccatcgcg ttacggccaa atttcgagtg atcgacaacc agcataacgt
     1801 ggcgcgagtt ctcaataatg gcgcgtttgg tgcgaacttc gtggtaatcg aactccagca
     1861 gcgagccgtc gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg
     1921 agataaaatc gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac
     1981 cggcgagaat aatgcggaaa tcttctttta ccatcaacgt gttagcaaca ttgagattgt
     2041 tggtgacaat acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg
     2101 tgccgatatc gataaacagt gtcgagccat tggggatttg ctccgccact ttgcgggcaa
     2161 tcctctcttt ttcttcggtc tgggtggctt tacgatcgtg ccacggcgtg ttaaccgaac
     2221 tggaaggcag cgccgcaccg ccatggtgac gcaggatcag gttttgctcc gccagctcat
     2281 tgaggtcgcg gcgaatagtc tgcgggctga cggagaaatg ctctaccagc tcttcagtac
     2341 tgacataacc ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca
     2401 tttataaatc cctggaatta ttttcgtttt cgcgccttga gcgaatcaac aaaagccatc
     2461 gctaaaccca cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa
     2521 tcaaaccatc cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa
     2581 atgccacttt gcggatcgcg ttcgccacgt agccagacgt agcccatcag cgcatacacc
     2641 acgccagaaa gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac
     2701 agggcgctaa tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgca
     2761 ccgccgagat accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt
     2821 aacgcgtggg tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc
     2881 agccataaca tcacttcctg atcgccgaga atttgcatgg cgataaacac caccacgcag
     2941 gcgatcatca ccacccaggt taccggacct gcgcgttcac gcaaggcagc aaagaaagga
     3001 taacggcgat aatgcagacc actgccggta tggcccgcct gccagctggc cgccagataa
     3061 cgcggatctg ccgggttttc gagaaaacgc gccagctccg cccgcacgcg ctcggcctgg
     3121 gactcatccg ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc
     3181 tgcgtcgcca tataatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc
     3241 atcaacatcg ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc
     3301 cgtacgccac ctctgcggga aactgacgct gccaggcttc aaaaccgccg tcaatgctat
     3361 agaccacatc gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattgccgt
     3421 gataacacat caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg
     3481 tgtcgttggt taaatggaaa gcctgcaccg catgccccat agcgaaactc tgtggatcgc
     3541 gaatatcgac cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa
     3601 tacattcgaa ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc
     3661 acagtttacg tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt
     3721 tttttcaatg ttacctaaag cgcgattctt tgctaatatg ctcgataacg aacatttatg
     3781 agctttaacg aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg
     3841 cggcatcaat ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat
     3901 gctggaggcg caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg
     3961 tggcctgcgc taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg
     4021 tgaagtgctg ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca
     4081 tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt acgatcatct
     4141 gggtaaacgc accagcctgc cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt
     4201 aaagccggaa attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct
     4261 ggtactcgcc aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg
     4321 cgccacctct gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac
     4381 cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa
     4441 acagttcttc gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg
     4501 cagccatatt gtggtgccgc gtgtgcatac ccagaagcag gcctacattc tgcaaaacga
     4561 agataaacgt attgtgttcg tgatcccgtg gatggacgag ttttccatca tcggcaccac
     4621 cgatgtggaa tacaaaggtg atccgaaagc agtgaagatt gaagagagtg aaatcaatta
     4681 cctgctgaaa gtgtataaca cgcactttaa aaagcagtta agccgtgacg atatcgtctg
     4741 gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aggctattac
     4801 ccgtgattac acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt
     4861 cggcggtaag ttgaccacct accgcaaact ggcggaacat gcgctggaaa aactgacgcc
     4921 gtattatcag ggcattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat
     4981 tgaaggcgac cgcgacgatt atgccgctcg cctgcgccgc cgctatccgt tcctgactga
     5041 atcgctggcg cgtcattacg ctcgcactta cggcagcaac agcgagctgc tgctcggcaa
     5101 tgcgggaacg gtaagcgatc tcggggaaga tttcggtcat gagttctacg aagcggagct
     5161 gaaatacctg gtggatcacg aatgggtccg ccgcgccgac gacgccctgt ggcgtcgcac
     5221 aaaacaaggc atgtggctaa atgcggatca acaatctcgt gtgagtcagt ggctggtgga
     5281 gtatacgcag cagaagttgt cgctggcgtc gtaaggatga tgcccggttg tgaagtgcaa
     5341 ccgggctatt tggattcctt agactattca ttgtaaataa ttgaataacg gcccctgagc
     5401 taaaatcgcc aaacttaagc acgttaccca atcgccccac ttcttcctct ggtttctatt
     5461 ttttctgcac caatggcaat aatatcccaa tgcaaaacca agctgtgcac taatcgggcc
     5521 gacaaaatca gtccaaggct gcggccaaaa aggggaaaaa ttcaggggaa gccagacaaa
     5581 cgtcagaaag aaatttcgga tattttccag caacgctgat ttctctggag aaatataaag
     5641 aatgaaattc gagagaatta accagaaata gaccggcgaa aagaccagcc agcaatgcag
     5701 tctgtttttt aattcaggag gcgttctgcg ggagaattgc caaccgatgc cagccattac
     5761 actgaaaaaa agtgcatatt caaccattaa ctcaagttgt agttcctgaa gagaaatacg
     5821 cacaaccagg agcggaaagg agaatattat ttctgccaga aaagttaacg tgaatataag
     5881 tacggctaag acaataaccg tcggccatac caataatggc acgataacaa aaccgagaat
     5941 tacaggggga atataaagcc ggaatttaat catttttatt ttcctggtga ttcggatgat
     6001 gcgtcataca tcgcgcataa acactcatca attatccagt catcttccca gacctgatat
     6061 tctgtcattg gttcgggctg ttttagcggg ttctcaatcg cttgccgcgc tctttctttc
     6121 agtaaaggat gattatttct agcccaggca ttgatttggg tggtaatgac gttatcgata
     6181 tcgtcctggt tatctctgat ataatcctca caatatctgt tcaataaaca ggcaagagaa
     6241 acagtcgacc aaaagccggg aactgaatta atggcatcca gcaatggcgc aagagcgata
     6301 tcggcacgga aaaacatttc cagcatatac tgtaattcga cagcttcatc gccggatacc
     6361 caatcagtca tatactggca ggcaaatcgc tgtaaaattg cccgttcatc cgcacgccag
     6421 ttttctttgt tggcaaggtt gatacggcgc agagaaaaaa ctacgtgaat attgtcaaaa
     6481 tgcccctgtg caacaaattc cagtaaacgc ggcagaaaat agcggacctc atcgctattt
     6541 tgcggatcag gccctgggct actattccag gcattgatta aggaatacgg gatcgctcgt
     6601 aacgatgtac tccgtaatgc cttttgttcc tgctgactca ggcaatattc acaacaaacc
     6661 acaaatttct gcggcacctg ataacgacga aaggtgtgat aaatctgctc aattaatgcc
     6721 tgcatggcgg ggctgggttg catagttacc ccctcgcccg ctgatcgcgt gtaaacggtt
     6781 gatagacaaa acgagcattc gccgcgttca ggcgaataag ggcgctacgc gaaagggaaa
     6841 agctggggcg aatgcaacta ttgcagtctc gacagctttc ctctgcgcaa ttttccactg
     6901 agtaatgacg cgtgtagagt ttcagttcgg cctgcttatc gcgaaatccg cgtaaactgt
     6961 tattgttatg aaatttccag gcatctttaa agacctgaaa gatatagaca tcctcaatcc
     7021 aggagtcaca ttttttaagc gggcgctttg ctattatcac ccgctcatcc ggatttagca
     7081 tcgccgtaaa ttgtacggtg acgtcatttg tcgtgttcag gtcacattga acatctggta
     7141 catcactgat ataggggtca aatccaaaat cagcaccaca ggcacaaaga cggctaatgg
     7201 tggcggtatc gagggaaaat cccggccata ccttgctgga ggttaagcca atatttaatg
     7261 tgacatcata tcgaagcaat ccctcaagac cgtcagcacc gacgaacttt tccccttcat
     7321 ccagggcctg gttaataagt tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt
     7381 tggcaatcgt aatatccacg cctgtgaagg gatcagtacc ggcaaggctt gcaaataatg
     7441 tattaactaa ttccattttt attatccagc tttaaggtag cgtcttatac atcgggacat
     7501 aacctacatc ttccaccaaa ctctgtccct gcggcgttaa aaaccactcc aacaattttt
     7561 gtgtctctga cgtcgtattt tcccgcgtta ccataaaggc atcgacgata tagggatatt
     7621 tgccgttgcg aatgttttcg gcagtcggtg caataccgtt aatcgctagc agttttatat
     7681 ttttatcggc gttcatttgt gttgcgtaat aacggaaggt gtaacctatt gcgttgtttg
     7741 tattacggta ttcggcaacg actttaatca tgccctccat catacttgcc acttctgttt
     7801 cctgcggcga gatcatgcgg acatttttca tgacctggga ttgcatcacc gtctggctac
     7861 cagagtcttc cgggcgttgc caggtctgga tctcctgatc gttaccgcca acagtaggcc
     7921 agttggtaat tgcaccactg aagatgtcac gcacctgttg ttcagtcagg gaattaaccg
     7981 gattatccgc attgacgatg aaaacaaacg cttcacgggc aaaaggcgtg tacagcaaag
     8041 tagcgcccga ttcctcagcg cgttttttct gcccaccgga aggttgcgcc acaaaaataa
     8101 tatcagcatc acctttaaca atcctgttat acgcatcggg ggtacgagag ctctccagat
     8161 attccctggt gtgaaaatct tccggtataa cgcttaaagc ataaaatgca gaggcataaa
     8221 ttgggtacgc cgccgtagca ccatcgagtc gcggccagtt ttgcgtaaac tgaatttgcg
     8281 gtttaccacg cagtggtgtc agttgattat tgagtttatc gggtcgccag gcccaggtat
     8341 caatattttc gcgcacaccg acgccgggat tgagctgttt atataaataa ccctgataac
     8401 cagcgacaaa accaagcatg agaaggataa acaggaggga attacggcaa cgtagcccgg
     8461 atgtaccggt taaaggatgt ctggttggcc ggccgtaccc cagcgaaaaa ctgatatgcg
     8521 cgccaacggg tataatgact atccaccagt actcacctcc gactactgag actaacaaaa
     8581 cgcccagcca ggggagcata agatcagcca accaacccga tcccatctgg tgaaattgat
     8641 aatctgccag atgcagcgcg attatccagc caaccaggat taataaaccg ggtactaaaa
     8701 aaagacattg tcgccagacg gctatttctc ttttttctgg tgcactatcg cctgcccata
     8761 ctcgccccga gaaaaatagc attaatgcgc ccagcgccag cataaaaatg ttgagcagaa
     8821 ggtaacttgt tggcgtgata aggatattac aaacttcaat aataccggca agtccgacac
     8881 ccagcatagc aacaaccgcc gccaaaaaat tgcgtcagta tggggatgcc gaaaaaagtc
     8941 attaccagcg aggacaaaat ccattttctg ttttgcatta ttctttccat tctttttgaa
     9001 tggtgaaatt atactccccg agtccccttg ccccttctgg acacttttcc gaaatgatgg
     9061 cggaaaaaaa cgggaccctt tggccccgtt ctatttattg gtgaacttac aatctcaccg
     9121 gatcgatatg ccagatatga tcggcgtact ctttgatagt acggtcagaa gagaagtagc
     9181 ccatattggc aatgttcagc atcgcttttg cggtccactc ttcctgaagc tcgtagagtt
     9241 cgtcgacttt atcctgacaa tcgacatagc tgcgataatc cgccagaacc tgatagtgat
     9301 cgccgaagtt gatcagcgaa tcaaccagat cgcgatagcg acccggatct tccggactga
     9361 atacaccgct gccgatttgc gtcagcacct gatgcagctc ctcatctttc tcgtagtatt
     9421 cacgcggttt gtagccctga cgacgcagtt cttccacttc ttccgcagtg ttaccaaaga
     9481 taaagatatt gtcagcaccg acatgatcca gcatctcgac gttcgcaccg tccagcgtac
     9541 cgatagtcag cgcgccgtta agcgcaaact tcatgttact ggtgccggaa gcttccgtcc
     9601 ctgccagcga aatctgttca gacagatctg ccgccggaat gatcaactgc gccaggctaa
     9661 cgctgtagtt cgggatgaac acgactttca gcttatcgcc aatctgcgga tcgttgttga
     9721 tcactttcgc tacgtcattg atcaaatgaa taatgtgctt cgccatgtaa taggccgaag
     9781 ccgccttacc gccaaaaata ttcacgcgcg gtacccactt cgcatccggg tcggccttga
     9841 tgcggttata gcgggtaatc acatgcaaca cattcatcaa ttgacgtttg tattcgtgaa
     9901 tacgtttgat ttgtacatcg aacaacgcct ttggattcac caccacattc agctgctggg
     9961 cgatatactc tgccagacgc tttttgttct ccagcttcgc ctgatgcaca gcgtgattaa
    10021 ccattgggaa atcacagtgt tgttgcagct cattaagcag gctaaggtcg gtgcgccagt
    10081 tacggcccag gtgttcgtcc agcacggctg aaagcgatgg gttcgctacc gctagccagc
    10141 gacgcggcgt cacaccgttg gtgacgttgg tgaaacgacc cgggaagatt ttcgcaaagt
    10201 cggcaaacaa cgattgcacc atcagattag agtgcagttc cgatacaccg ttaactttgt
    10261 ggctcacaac aaccgccagc caggccatac gcacacgacg accgttggat tcatcaatga
    10321 tcgacgcccg tcccagcaga tcggtatcgt tcggatactg ttcctgcaag gttttcagga
    10381 aatagtcgtt gatttcaaag atgatctgca ggtgacgcgg cagaatttta cccagcatat
    10441 caaccggcca ggtttccagc gcctcgctca tcagcgtgtg gttagtgtag gagaagacct
    10501 gacaacacac ctcaaacgcg tcgtcccagc taaattggtg ctcatcgatc agcagacgca
    10561 tcatctcagg aatcgacagt accggatggg tatcattgag atgaatcgcg attttatccg
    10621 ccaggttatc gtaggtttta tgcaactgat aatggcggct taaaatgtcc tgaatggtcg
    10681 aggaaaccag gaagtattcc tgacgcaggc gcagctcacg cccggagtag gtggagtcat
    10741 ccggatacag tacgcgagat acgttctcgg agtggttttt atcttccact gccgcgaagt
    10801 agtcaccctg gttgaattta ccgaggttaa tttcgctact ggcttgcgca ctccacaaac
    10861 gcaacgtgtt ggtcgcgtcg gtgtcgtaac cagggattat ctgatcgtaa gcgactccca
    10921 gaatctcttc ggtttcaatc cagcgcgttt ttttaccttc ctgctgaatg cgaccaccaa
    10981 aacggacttt atagcgcgtg ttgtggcgtt tgaattccca cgggttaccg tattccagcc
    11041 agtagtctgg cgactctttc tggctaccgt taacgatgtt ctgcttgaac ataccgtagt
    11101 catagcggat gccgtaaccg cgccccggca accctaacgt cgccagagaa tcaaggaagc
    11161 aagccgccag acgtcccagg ccaccgttac cgaggcctgg gtcattttct tcatcaatca
    11221 gctcttcgag atttaacccc atcgcttcca gtgcgccctg tacatcttcg taaattccta
    11281 gcgacaacat ggcgttggag agcgtacggc caatcaaaaa ctccatcgac aggtagtaaa
    11341 cctgacgagt ttcttgcgac aactgggcac ggtttgaacg taaccagcgc tccacgagac
    11401 gatcgcgcac agcaaataac gttgcgttca gccattcatg tttattggcg acgaccgggt
    11461 cctttccaat cgtaaacatc agcttgtaag cgatagagtg cttaagagct tctacgctaa
    11521 gcgtgggcga tgaatatgta aacggagcat tcatataggc gtttcctgaa aactatttca
    11581 agcgatagta aagctcacgg tacgacttcg ccgcgacctg ccagctaaaa tccattgcca
    11641 tagcctgacg ttgcacaaac cgccacagtg aaggacggga ccacagtaca aaagcacgtc
    11701 gaatagcccg taacagcgac caggcattac tatcttcaaa gacaaaccca ctggcgacgc
    11761 catctgcaag gttctcaaga gaacagtcag aaaccgtatc agcaagccca ccggtgcgcc
    11821 gcactaacgg cagcgtaccg tacttcaatc cataaagttg cgttaagccg cacggttcaa
    11881 aacggctggg caccagaatg acgtccgcgc cgcccataat gcgatgcgaa aatgcttcgt
    11941 gatagccaat ctgaacgccc acctggccgg ggtattccgc tgccgccgca aggaaacctt
    12001 cctgcagcac cggatcgccc gcgccgagta gcgccagctg cccgccctgc tccagaagac
    12061 ccggtaaggc ttccagcacc aggtcgagac ctttctggct ggtcagacgg ctcaccactg
    12121 caaaaagcgg cactttatcg tcaaccttaa gccccattgc gatttgtaac tggcgcttat
    12181 tttccgcttt atcttccaac gtatcgcggg tgtagcgcga ggccaacagt aagtccgtct
    12241 ctggactcca gattttctcg tccacgccgt tcagtacgcc ggaaagacgc ccttcacggt
    12301 gacgctgttg caacagacct tccataccgt aggcaaactg cggttcggtg atctcgcgag
    12361 cgtaggttgg actgaccgcc gtaatgtgat cggcatagta cagaccggcc ttcaggaaag
    12421 agatttgtcc gttgaattcc agcccatgaa tattaaagaa tgaccatggc aattggatgt
    12481 cattcatatg atgtgcataa aacatgcctt gataggccag gttgtgcaca gtaaacaccg
    12541 acttcgccgg acgcccgcgc gccgccagat acgcaggcgc aaggcctgca tgccagtcgt
    12601 gcgcatgcac cacatcagga cgccagaatg ggtcaagccc gctggccatt tctgccccaa
    12661 cccaccccag cagcgcaaaa cgcaatacgt tgtcggtata ggcaaataag ttggtatcgt
    12721 gatacgggct tcccggacga tcatagagat gcggcgcgtc aatcaggtat atgccaaccc
    12781 cgttgtaatg accgaacaac agcgtgatat gtccggcgaa ggtatcacga cgggatacta
    12841 cctgcgcatc ggtcacgcca cggcgaatat cgggaaatgc aggcaacagt acgcgagcgt
    12901 caacgccgtc tgcgatttgt gctgcgggta atgccccaat aacatcagcc agaccgccgg
    12961 ttttaagcag cgggaacatc tctgaacata catgtaaaac ctgcattatc gctcctgttt
    13021 atgccctaac ttccgtagca tttcgcgcgt taccagcacg atgccttctt ctgaacgata
    13081 gaaacgacgt gcatcttcct ctgcgttttc accaatcacc atgccttccg gaataacaca
    13141 agcacgatcg atgacgcagc ggcgcagacg gcacgagcga cctacccata cttccggtaa
    13201 caatacggcg gaatcaatgt tgcagaatga attcacgcga acgcgcgaga acagaacgga
    13261 ctgcaccacc accgaaccgg agatcacaca gccgccggat accagtgagt taagggtcat
    13321 cccgtggcta ccggagcgat cctgcacgaa tttcgctggc ggtaatgatt cattgtaggt
    13381 gcgaattggc caattgcgat cgtacatatc cagttccggc accacagagg ccagatcgag
    13441 gttcgctttc cagtaagctt ccagcgtacc cacatcgcgc cagtacggct cggcatccgg
    13501 gtcggattgt acgcaagaga gcgggaacgg gtgcgcatag gccagaccgg cttcggtgat
    13561 cttgggaatc aaatctttgc caaagtcgtg gctggagttc tcatcacgat cgtcttcttc
    13621 cagcagttca tacagatagt cggcgtcaaa gacgtagata cccatactcg ccagagattt
    13681 gctcggatcg ttcggcattg acggtgggtt agcaggtttt tcaacgaatt cgataatttt
    13741 atcgttctca tcaaccgcca taacgccaaa tgcggaggct tcttcaatcg gtactggcat
    13801 acaagcaacg gtgcaacgtg cgcctttttc gacgtgatcg ataagcatac gcgagtagtc
    13861 ttgcttgtag atatggtcgc ccgccaggat caccacgtat tccgctttat aacggcggat
    13921 aatgtcgagg ttttgggtga ccgcatctgc ggtgccgcga taccagtttt cccctttcat
    13981 tctctgctgt gctggcagca gatcgacaaa ctcgttcatt tcttcattga agaatgacca
    14041 gccgcgctga atgtgctgca ccagagtgtg ggactggtac tgggtgatca cgcccatacg
    14101 acggatccca gagttgatgc agttagacag cgcaaagtct ataatgcgga acttaccgcc
    14161 gaagtgtacg gccggttttg ctcgcttatt ggttaaatcc ttcaggcggg taccacgtcc
    14221 tcccgccagt atcagggcaa cagatttcaa tggcagctgg cgcgccaaca ttaagtgatc
    14281 gttcttctct aaactaacca tgactaactc cttttttatc atctctggaa cacacacaat
    14341 ccgtgtgcag gtccctgcca gacagccgta atcactgggt tatcctctcc agcgaatggg
    14401 ggaatggcgt gccactcccc agcaggtaaa acaatctctg ttacctcaag cgtggcgtta
    14461 attgcgatca aaaagcgatc cgagagcaga atttgcagct gtttcggccc gttttgccac
    14521 tcatccgtgc ttaaaggttg agcatatcga tttagccaac ggacattgcc gtcgccttct
    14581 tcccaccagc gattctccac caaagcggga atacgcttgc gcagatggat taacgcggcg
    14641 gtaaatgcgg ttaaaccact gcttgcctgc gaccagtcca accaggttaa ttggttatcc
    14701 tgacagtagg cattgttatt gccatgctgg ctgtgaccat gttcgtcacc ggccagtaac
    14761 atcggcgtac cctgggagag caacaacgtt gttaacaggg cgtgaatact gtcgcgccgc
    14821 cgttcaacca ggtcaagaga accgcctaac ccttctttac catgattgtt actgtaattg
    14881 ttgttggtcc cgtcgcgatt ttcttctccg tttgcttcat tgtgtttatg gttgaagcaa
    14941 acgcagtcgc gaagcgtaaa accgtcatgc gcggtgacga gattaatcgc ggcactcggc
    15001 agacgaccat tacgtttaaa aacatcgctg gaggcagcaa aacgcccggc aaacgccccc
    15061 agaggcaaat cataatgtag ccagaaacga cgggcagcat cgcggaaatg atcgttccac
    15121 tcggcaaaca gcggcgggaa atttcccacc tgataaccac caggagcgat atcccacggt
    15181 tcagcaatta acttcacctg cgagagcacc gggcagttct ggatagcggt aaacaacggc
    15241 gcatcctgac ggaactctgg cgtacggccc atgactgccg ccagatcaaa gcggaaacca
    15301 tcgacgtggc aggtttctac ccaataacgc aggcaggcgc tggcataatc caccaccgcc
    15361 ggatgactca aattgagcgt gttgccgcaa ccggtccagt tgtgataatc gccgtcttct
    15421 cttatccaat aatagctacg gttatcgatc ccacgcagcg agaataacgg gccgtcgagg
    15481 tccagttccg cactatggtt gagcacgata tcaagaatga cttcgatacc cgctttatgc
    15541 agtgctttga ttgcatcgcg aaactcatcc agcgccgttt ctggcgagca ggcatacgcc
    15601 ggatgcagcg caaacatcgc caccgggttg taaccccagt agttacttag ccccatgcgt
    15661 tgcagacgtg gttcactggc aaactgcgcc actggcagca gttccagcgc ggtaatgccc
    15721 aattgtttca aatagttgat catcaccgga tgcccgaggg ctttataagt gccacggatc
    15781 tcgaccggga tctccgggtg caagtacgtt aatcctttga catgggcttc ataaatgatg
    15841 gtgctgcccc acggcgtgcg cggcggggca tcatcttccc agtcatagtg atcaaccacc
    15901 actacgcatt tcggcgcaat ggcggcgttg tcgcgatagt caggttcatt atgaccggcg
    15961 tgcagcagcg ggttatcttt aaactccccg tcaatttgcc gcgcgcaagg atcaatcaac
    16021 aacttcgccg ggttaaagcg atgcccctcg gcgggttgcc aggggccatg aacgcgataa
    16081 ccataacgca aacccgggcg cgcatccggc agataaccgt gccaaatgtc gccactgtgc
    16141 cctggcaagt catagcgatg ttcctggcca ttggcgtcaa agacacacag ttctacccgc
    16201 tcggcatgag cggagaaaag tgtgaagttg acgccctgac cgtcgtaatg cgcgccgagg
    16261 ggagcgggtt tgccaatggc gagttgtgtc attctgcctc ccgaaccagc cagatagtgg
    16321 ccagcggtgg tagtgtcagg cttagtgaat gctgacgacc gtggctggca atctcatcgc
    16381 tgtgtaccgt gccgccattg cctgcattac tgccgtgata gtgcatggaa tcggtattga
    16441 ggatttcacg ccatttgcct ggctgattaa tgccgaagcg ataatcatga cgcggcaccg
    16501 gcgtaaagtt actggcaacg ataatttcgt taccctcttt atcgcgacgc acaaagatca
    16561 gcaccgagcg ttctttgtca tccaccacca gccattcaaa gccgtacggg tcaaaatcca
    16621 gttcatgcat tgctttatgg tggcggtagg tgaggttcag atcgcgcacc agacgctgga
    16681 caccgtggtg ccagttatcg ccgccttcca acagatgcca gtcgaggctg gcgtcatggt
    16741 tccactcacg gccttgggca aattcgttcc ccatgaacag cagtttcttg cccgggaatg
    16801 cccacatcca gccatagtag gcgcgcaggt tagcgaattt ctgccatgcg tcgcccggca
    16861 tgcggtcgag aatcgatttt ttaccgtgga ccacttcatc atgcgacaac ggcaggacga
    16921 agttttcagt gtagttgtag agaatcccga aggtcagttt atcgtgatga tactgacgat
    16981 aaaccgggtc gagcttcatg tagtccaggg tgtcatgcat ccagccgagg ttccacttgt
    17041 accagaagcc cagaccgccc atatcctgcg gacgagaaac gccagggaaa tcggtagact
    17101 cctcagccat tgtcaccgca ccggaaacct gctcaccaag aatacggttg gtattacgca
    17161 agaattcaat cgcttcaaga ttctcgcgcc cgccaaattc gttcgggatc cactccccct
    17221 ctttacggct gtagtcgcga taaatcattg acgccaccgc atcgacgcgc agcgcatcaa
    17281 taccaaaacg ttcaatccag taaagcgcgt taccgacgag gaagttactg acttcacggc
    17341 gaccatagtt gtagatcagc gtgttccagt cctgatgata gccttcacgc ggatcgctgt
    17401 gttcatacaa gttcgtgcca tcaaattcgg caagcgcaaa gtcatcagtc gggaagtggc
    17461 ctggcaccca gtcgagaatc acgttcagac cagctgcgtg tgcggcatca atgaaataac
    17521 ggaagtcgtc gcgagtacca aaacggcggg ttggtgcata caggccggtt ggctgataac
    17581 cccaactgcc atcgaaggga tgctcgttaa tgggcagtag ttcgaggtgg gtaaagccca
    17641 tccatttagc ataaggcacc agttgatcgg ccagctcgcg gtagctcaac cagaaattgt
    17701 tgtcggtgtg gcgacgccag gaacccaggt gaacttcata aatagagatt ggcgcatcaa
    17761 actgattcgc ttttttgcgc tcttcagtct gtacaacctt ttccggcagc ccgcaaataa
    17821 gagacgcggt ttccgggcgc atttgcgctt cgaaggcata agggtcggac ttcagacgca
    17881 agttgccatt ggcatcaatc atctcgtatt tatagagctg accgttatgc gccccaggga
    17941 taaacagttc ccagatgccg ctctctttac gcaggcgcat cgggtgacgg cgaccgtccc
    18001 agtagttgaa ttgcccaacc accgagaccc gacgggcgtt tggagcccag acagagaaac
    18061 gcgtacctgt gacgccatcc atagtatctg catgcgcgcc taaggtttca tacgggcgca
    18121 ggtgagtacc ttcagataat agccaggcat ccatttcctg gattagcgga ccaaaacggt
    18181 aaggatcatc aatcaggttt tgctgaccat gccagacaac agccaactga tagcggaaaa
    18241 aattcttacg tcgcggaatg acgccgctaa agaatccccg tgagtcgaga cactccagtt
    18301 ttgcgagttt gcgcccggtt ttcggttcaa tcacccacac atcggtagcg tcgggtaaaa
    18361 gggcacggac ttccagtccc gcggtggttt tatgcattcc cagtacggaa aaaggatccg
    18421 caaaatggcc tgcaattagc gcgttaatca cgtctctatc gatacgatcg gacatgcttg
    18481 tcttcctgtt ttattgtgtc accccatcca attggatttt tgacttctgg ttgtgacatt
    18541 tttttgacct gaacggcgca gcactctgtg catcctctct gcgtcgtcct cacttcaggt
    18601 aaggctgtga atactcatgt attcagccac ccttaaagaa tagccaatgc tctatttaac
    18661 tcccggtaaa tcatgaaaca tctgcgctta ctcctgtatt acgcactaac aggggcggca
    18721 tcgcgcccca gatttaatga ataaagatta cgccagttga cgaagcatcc gacgcagcgg
    18781 ctccgcggcc ccccacagca gctggtcgcc cactgtaaag gctgacagga actctggtcc
    18841 catattcagc ttacgcaggc ggcctaccgg cgtggtcagc gtgccggtaa cggcagctgg
    18901 ggttagctca cgcatagtga tttcccgatc gttcggaacg actttcgccc acggattgtg
    18961 cgcagccagc agttcttcca cggtcggaat agacacatct tttttcaatt taatagtgaa
    19021 tgcctggctg tggcagcgca atgccccgac acgcacacat aaaccatcta ccggaattac
    19081 ggaagatgtg ttgaggatct tgttggtttc cgcctgccct ttccactctt cgcggctctg
    19141 accgttatcg agctgtttgt cgatccacgg aatcaggcta cccgccagcg gcacgccaaa
    19201 gttatccacc ggcagctcac cgctacgggt taaggttgtg actttgcgtt cgatatcgag
    19261 aatagcagag gacggggtcg cgagttcatc tgccacatgg ccatacagat ggcccatctg
    19321 ggttaataac tcacgcatat gtcgcgcacc accgccggaa gcggcctggt aggttgcaac
    19381 ggacacccaa tcaacaagat cattggcgaa taaaccaccc aacgacatca acatcaggct
    19441 tacggtacag ttaccgccaa caaaagtcct gatgccatta tttaatccgt cggtaatgac
    19501 gtcctgattg acggggtcaa gaatgatgat ggcgtcatct ttcatgcgca gagacgatgc
    19561 tgcgtcaatc cagtaacctt gccatccgct ttcacgaagc tttggataga tttcgttggt
    19621 ataatcgccg ccctgacagg tcacaatgat atcgagggcc tttagcgcct ccagatcaaa
    19681 ggcatcctga agtgtgccag tggttccgcc aaaagacggc gcagcctggc caagctgaga
    19741 agtagaaaag aagacagggc gaatggcgtc gaagtcgcgc tcttcaacca tgcgttgcat
    19801 gagaacggag ccgaccatac cgcgccagcc gataaaacca acatttttca taagcgtttt
    19861 ttcctgcaaa gatgtgtgct gtataaatgt gccggtctcc tcttggcaca tctttcacca
    19921 tacaaaaagc agccaaagtc gcaagtgaaa ttaatcaatg atagcgaagc catcagtaat
    19981 gcgacttatc ctgctttgtt agcacgcaga aagtccgcgg caattatcag ggaatttgag
    20041 ttatgaatga aatcatttct gcagcagttt tattgatcct gattatggat ccgctcggaa
    20101 acctgcctat tttcatgtcc gtactgaaac atactgaacc gaaaagacgg cgggcaatca
    20161 tggtgcgaga gttgcttatt gctctcctgg tgatgctggt gttcctgttt gcgggtgaga
    20221 aaattctggc atttctcagc ctgcgagcag aaaccgtctc catttctggc ggcatcattc
    20281 tgtttctaat cgccattaag atgattttcc ccagcgcttc aggaaatagc agcgggcttc
    20341 cggcaggtga agagccattt atcgtgccgt tggcaattcc gttagtcgcc gggccgacta
    20401 ttctcgccac gctgatgttg ttgtctcatc agtacccgaa tcagatggga catctggtga
    20461 ttgctctgct gctggcctgg ggcggcacct ttgtcatcct gctacagtct tcgctatttt
    20521 tacgtctgct gggcgagaaa ggggtgaacg cacttgaacg cctgatggga ttgattctgg
    20581 tgatgatggc aacccagatg ttcctcgacg gcattcgaat gtggatgaag gggtaacgag
    20641 gagagttttt cgaaaagaac ccggtagtgt cacaactacc gggcgaacaa acttaactca
    20701 acagctggaa cgcaatcatc ccaacgatgg caccgacagt gccaaggatg gtttccatca
    20761 tggtccaggt tttcagcgtt tcggcttcgg tcgcgccggt aaatttaccg aacaaccaga
    20821 aaccggcgtc gttaacgtgg ctgacaacaa tcgaaccacc agcgatacaa atcgacagcg
    20881 ccgccatttg cgcaccggag tagttcagtt gttcaataac cggcatcacc agtcctaccg
    20941 ccgttaaaca ggctacggtg gcagaaccct gaatgatgcg cactgcagct gccagcacga
    21001 agcaggtgat agcaatcggc aggcccatgc cagttaacgc ttcgcccagt gccggaccta
    21061 cgccagagtc aaccagcacc tgtttgaaca cgccgcctgc accaatcacc agcagaatga
    21121 tccccgccgg ttgcagcgcg tgaccgcaaa tctccatcac tttgtctttt ggcatgccct
    21181 gacgcattgc caggccataa atcgctacca gacaagcaac cagaatcgcg gtaaacggat
    21241 gaccaataaa ctcgaaccat tcgtaagcgg tagagccttc tggcacaaaa cgcgcggcaa
    21301 tggttttcag ccccaccagc accagcggca acaggatcag cgacaggctg aatccgaaag
    21361 atggcatttt gccttcgccg agatgcggtt cgctgatgtc gtcaggaata tgcaactcca
    21421 cgtagcggct gatgaaatta ccccacagcg gcccggcaat aatcattccc ggaattgccg
    21481 cacacaggcc aatcaggatc atccagccaa aatcggcgtt catctgcgat gccagcagca
    21541 ttggcgctgg ccctggcacc aggaacgccg ccgctgccgc cacgcctgcg aataatggga
    21601 ttaccagctt caccaggttc gtaccggtgt ggcgcgccat tgagaaagca acgctaatca
    21661 gcagaactat cgccacttca aagaacagcg gtagcgcaca gaccaacccc gcaaggccga
    21721 tggcataatg cgcgcggctg tgaccgaagg atttgagcat tttgacggca atctgatcga
    21781 ctgcgccggt ttcatgtaag atcttgccaa acatagctcc cagggcgaca accaccgcca
    21841 ggaagccgag ggtgcctccc atcccttttt ccatcgtcgc tgcgatttta tcgagcggca
    21901 taccggaaaa aaggccagcc cccatggaca ccaccatcaa agccaggaaa gcgtgcatac
    21961 gcgccttcat gactaaaaac agcagcagta aaacagaccc tactgctgtt aaaacaagcg
    22021 ttaatgtagt cactacttat ttgccttttt taataacctc aatggtgctt gccacaacac
    22081 cttccagcgg ttgatcgata tccaccacca gtacatcggt ttcgtccgca cccggctcct
    22141 gcagcgtttc aaactgcgtc accaacattt gggttttaaa gaaatggcct ttgcgcgctt
    22201 tcaagcgact ttcaatcaca tcaaaatcgc ctttcaggta gatgaaagag agattcggat
    22261 taccttcacg cagcaagtcg cgatagtgct ttttcagtgc agaacagacg atcagcgaca
    22321 ctttattggt gcgctgcata gcaaacgcgg cgtcgttcag cgcctgcagc cacggtttgc
    22381 gatcgtcatc atgcagcggt tcgccagacg ccattttttc gatattgcgc cgtggatgga
    22441 ggaaatcgcc atcaagaaac gcggcatgaa gttgatgcgc cacttcactg gcgaccgcag
    22501 atttgccgct gcccgatacg cccatcaaga cgtaaatgtg gtgatcatgg ttagtcgtgc
    22561 tcaaagtggt gcccccacaa tacaagaatt aaaaatgtta cgggtaactg ttatcggtaa
    22621 cattgtccag ccggacaata tcagaagcaa tatccatccg tgccttaagt gtataagtgt
    22681 gagctacttc aaatttgtag gcttaaatag atccgcccgg tgacaaggtg aaacctaaat
    22741 ctaacatttt cggtgtcaca gattcgccac gaatacgcgc cagcaggcgt tcagcgccaa
    22801 tactgcccat ccgctcacgc ggcgtcagca cgctcgctaa ccgcggctcc atcacctggc
    22861 caatgtcatg accgtggaaa ccggcaatcg ccatatcgtc aggaaccttc aatcccagac
    22921 gctgacattc aaacgccgcg ccgaccgcca ggtcatcgtt ggtacagaac acgccatcca
    22981 gctgcggata ttcccgccgc gcctggcgaa tcagttcaat accggaagag taagaagaag
    23041 attgctcaac catcacgcta tacggcacca gacctgcatc cagcatcgcc tgttcgtatc
    23101 ccttctgttt gatgatagta cgttcgtcga gacgcgcgcc gagataggca atgtggcaat
    23161 gcccgcgagc aataatggcg gtggtcatct ggcgtgccgc ttcaaagtta tcaaaaccga
    23221 cggcgatatc gaggcacggc gactggctgt ccatgagttc caccaccggg atccccgcca
    23281 cttcaatcat ctttaaggtg cgcggcgtgt gggtacgttc ggtgaggatc agtccatcga
    23341 tattccatga gagcatggat tcgaggcgtt cttgctccat ttccggttta taaccgtagt
    23401 gcgccagcat ggtttgataa ccgtgcgcgt cggtgacgct ttcgattccg cgtaatactt
    23461 ccgcgaaaac ctggttggtg agagaaggta acaggacgcc aatcgcccgg ctggtagcgt
    23521 tagagaggat atcgggcgca cgattgggaa tatagcccag ttcatcaaga gcggcggcaa
    23581 tcttgccgcg tagagcgacg gaaacctgct ccgggttgcg taaaaaacgg ctgaccgtca
    23641 ttttggtcac gcctacacgg tcagccacat cctgaagtac gggtcttttc tttttcatcg
    23701 tcctgaaggt acaaaagaga tagattctct tagtttaaca cggacgcatc agaaccttcc
    23761 cggggaaaac agtgcttgtt tacaattatt agatatacac taaatcaatt gcatcgcatt
    23821 gtgctaacga cgcggcaaaa ccccggaagc gtgcatcagc acgcgaccgg agcgagcgag
    23881 gaagcctgca aaaaggcagg cttcaaaaat gccgtggagc tttaaaccgg cggcagatcg
    23941 aacagtaaca cttcgctgtc gctatccgca tggatggaaa ttgcctgctc atcccagatt
    24001 gccagaccat cgctggtcga ggctttcacg ccgttaatgg tgacattgcc tttcaccacc
    24061 tggatccaga cgcggcgttc agcggcaatc tgatgcaccg actgctcatc tttcagcaac
    24121 gcccaacgat acagttccat atcctgatgc actttcagcg aaccatctcg cgcatccggc
    24181 gagagcacca gctgtttgcc ctgcacggca tcgaagcgac gctgttcata acgcggcgta
    24241 atgccatttt cttcaggcat gatccagatc tgatacagat gcagacgctc ggtgctgttt
    24301 gggttgtact ctgagtgacg aatacccgta cccgcactca taatctggaa ctcacccgcc
    24361 ggaacctgct ctttattgcc catgctgtcc tgatgctcaa cagtaccttc cagcacgtag
    24421 gtcaaaattt ccatatcttt atgcgggtga gtgccgaagc cctgccctgc ttcaatcacg
    24481 tcgtcgttaa tcacgcgcag cgcggagaag cccataaagt tcggatcgta atagttggca
    24541 aaagagaaag tatgccagga gtccagccag ccatgatttg catgaccacg ttcatttgct
    24601 ttgcgtaagt agatcatttg tattcgcccc ctgaatgatt tcgatgggct tagtgtggac
    24661 ccgatctgcc tgggatgata gagggtgaaa attgacccct ctgttcaaaa aaattgaact
    24721 attcgagggg ccatcaaact tacttagcga gagttactgt ggagggagag gcttgctcaa
    24781 atccgcgttc aaggatttcc agattggtaa gaacttcaga ttccttgacg taatttggcg
    24841 caccgtgggt gatggtttga tacaacgcat cataaacgcg cccgtaatcg cccatctccg
    24901 gcttcatctc ttctctgacc gtcacgccct cgtcattgac atactccagc acaccgaccg
    24961 aatcatccgc tgcgaatccc ggttcgcccg gcataatatt agccttcagg ctggtttcct
    25021 gctggtcgat accgtattta ataaacgaac ctttcttacc gtgaacgata aatttcggat
    25081 aatcgatttt caccagatgg ctggttttga cgatggcttt caggtcgcca taaaacagtt
    25141 gcgcttcaaa ggtgtcgtca ggattggctt tattacgcag gctgcggatg tcataagcga
    25201 cgtgatccgg gcgaccgaac agagaaataa tctggtccat cgtatgcaca ccaaggccat
    25261 aaaacgcgcc atcctgcggc agcccaggtt tggtttctgc caccgggcgg taatagtcaa
    25321 aatggctttc cacttcaaca atctctccca acttgccact ttcaatcgct tttttcgctg
    25381 tcaggaagca ggagtcaaag cgacgattct gatacggcgt gacggtcagc cctttgcttt
    25441 tcgccaacgc aaacagctcc ttcgcctggg caagcgtcgg ggtgaacggt ttttcgacca
    25501 gcacattttt cccggcttcc agcgcgcgtt tcgcgtcctc gaaatggctg tccgcgtggg
    25561 tgcagacaac aaccagctta acatcgggat cgtttagtac ttcgtcgaga tcgctggtga
    25621 aatggatatg ggaataaatg ggagcctgtt cttccggctt cgcatggcga cgaaaaatat
    25681 gcgcgacatg ccagctatcc ttgcggttaa gtacatacgg cagatggtaa cgggtggtgc
    25741 ttttgccgaa gccaataaag gcgcagttga tgaccatgat ttcgtccttt ttaaggtggt
    25801 tatttacacc ttagcgcaaa gcggacgtgg ttcctactgg agtgcgcata acgaacacaa
    25861 gcactcccgt ggataaattg agaacgaaag atcaaaaaaa aagccagcac ccggctggct
    25921 aagtaatact ggaagcaatg tgagcaatgt cgtgctttca ggttctccgc gagggtcttc
    25981 ctgatcgcga gacaataata atcattctca ttcgcacttg tccaacactt tttgcaaaaa
    26041 aatgcatttg actcgcattt gaaagtcaat gatgttgaaa gggacattta ccccaaagag
    26101 gacaaaggaa tgagtgagat agtaatacgc cacgcagaaa cacgggatta cgaggacatc
    26161 aggcagattc acgcccagcc ggaggtgtat tgcaacacac tacaggtgcc tcatccttcc
    26221 gatcatatgt ggcaggagcg actcgccgat cgtcccggca tcaagcaact cgtcgcctgt
    26281 attgatggag acgtcgtggg ccatctcacc attgacgtgc aacagcgccc acgccgcagt
    26341 catgttgccg attttggtat ctgtgtcgac tctcgttgga agaaccgcgg cgtcgccagc
    26401 gccctgatgc gagagatgat tgaaatgtgc gacaactgat tgcgggtaga tcgcattgaa
    26461 ctaaccgtgt ttgtcgataa cgccccggca attaaggtct ataaaaaata cggctttgaa
    26521 attgaaggga ctggtaagaa gtacgcattg cgtaatggtg aatatgtcga tgcatattat
    26581 atggcgcggg tgaagtaaga tagtgccctt tttctgagat ggataaaggg tgtcattcaa
    26641 aatcgacata ccttccttta aggtatttat ttgcccaata catatattga gatttaactc
    26701 ataattactt cctaaagtgt aatattttat tttttaatat atacgcctac aatttcctgg
    26761 agtaaataaa taacaattaa caagcataat attgccattg ataaaatagc atgccataaa
    26821 aggacttttc agggatgagt aatattgttt acctgacagt aacgggagaa caacaaggaa
    26881 gcatctccgc aggttgtggg acttctgagt ctacaggtaa tcgttggcag agcgggcatg
    26941 aggatgaaat a
//
LOCUS       DAZKIV010000053        26349 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZKIV010000053 DAZKIV010000000
VERSION     DAZKIV010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.053"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..231
                     /gene="tuf"
                     /locus_tag="ACHOT6_002171"
     CDS             <1..231
                     /gene="tuf"
                     /locus_tag="ACHOT6_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFP9947387.1"
                     /translation="RHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVT
                     LIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            414..608
                     /gene="bfd"
                     /locus_tag="ACHOT6_002172"
     CDS             414..608
                     /gene="bfd"
                     /locus_tag="ACHOT6_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFP9947388.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            681..1157
                     /gene="bfr"
                     /locus_tag="ACHOT6_002173"
     CDS             681..1157
                     /gene="bfr"
                     /locus_tag="ACHOT6_002173"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFP9947389.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1154..1621)
                     /locus_tag="ACHOT6_002174"
     CDS             complement(1154..1621)
                     /locus_tag="ACHOT6_002174"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9947390.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFTCGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHOT6_002175"
     CDS             2000..2311
                     /gene="rpsJ"
                     /locus_tag="ACHOT6_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFP9947391.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2344..2973
                     /gene="rplC"
                     /locus_tag="ACHOT6_002176"
     CDS             2344..2973
                     /gene="rplC"
                     /locus_tag="ACHOT6_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFP9947392.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2984..3589
                     /gene="rplD"
                     /locus_tag="ACHOT6_002177"
     CDS             2984..3589
                     /gene="rplD"
                     /locus_tag="ACHOT6_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFP9947393.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3586..3888
                     /gene="rplW"
                     /locus_tag="ACHOT6_002178"
     CDS             3586..3888
                     /gene="rplW"
                     /locus_tag="ACHOT6_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFP9947394.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3906..4727
                     /gene="rplB"
                     /locus_tag="ACHOT6_002179"
     CDS             3906..4727
                     /gene="rplB"
                     /locus_tag="ACHOT6_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFP9947395.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHOT6_002180"
     CDS             4744..5022
                     /gene="rpsS"
                     /locus_tag="ACHOT6_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFP9947396.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5037..5369
                     /gene="rplV"
                     /locus_tag="ACHOT6_002181"
     CDS             5037..5369
                     /gene="rplV"
                     /locus_tag="ACHOT6_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFP9947397.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHOT6_002182"
     CDS             5387..6088
                     /gene="rpsC"
                     /locus_tag="ACHOT6_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFP9947398.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6101..6511
                     /gene="rplP"
                     /locus_tag="ACHOT6_002183"
     CDS             6101..6511
                     /gene="rplP"
                     /locus_tag="ACHOT6_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFP9947399.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHOT6_002184"
     CDS             6511..6702
                     /gene="rpmC"
                     /locus_tag="ACHOT6_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFP9947400.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHOT6_002185"
     CDS             6702..6956
                     /gene="rpsQ"
                     /locus_tag="ACHOT6_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFP9947401.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7121..7492
                     /gene="rplN"
                     /locus_tag="ACHOT6_002186"
     CDS             7121..7492
                     /gene="rplN"
                     /locus_tag="ACHOT6_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFP9947402.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7503..7817
                     /gene="rplX"
                     /locus_tag="ACHOT6_002187"
     CDS             7503..7817
                     /gene="rplX"
                     /locus_tag="ACHOT6_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFP9947403.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7832..8371
                     /gene="rplE"
                     /locus_tag="ACHOT6_002188"
     CDS             7832..8371
                     /gene="rplE"
                     /locus_tag="ACHOT6_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFP9947404.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHOT6_002189"
     CDS             8386..8691
                     /gene="rpsN"
                     /locus_tag="ACHOT6_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFP9947405.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHOT6_002190"
     CDS             8725..9117
                     /gene="rpsH"
                     /locus_tag="ACHOT6_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFP9947406.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9130..9663
                     /gene="rplF"
                     /locus_tag="ACHOT6_002191"
     CDS             9130..9663
                     /gene="rplF"
                     /locus_tag="ACHOT6_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFP9947407.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9673..10026
                     /gene="rplR"
                     /locus_tag="ACHOT6_002192"
     CDS             9673..10026
                     /gene="rplR"
                     /locus_tag="ACHOT6_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFP9947408.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHOT6_002193"
     CDS             10041..10544
                     /gene="rpsE"
                     /locus_tag="ACHOT6_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFP9947409.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHOT6_002194"
     CDS             10548..10727
                     /gene="rpmD"
                     /locus_tag="ACHOT6_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFP9947410.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10731..11165
                     /gene="rplO"
                     /locus_tag="ACHOT6_002195"
     CDS             10731..11165
                     /gene="rplO"
                     /locus_tag="ACHOT6_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFP9947411.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEVAGGKIEE"
     gene            11173..12504
                     /gene="secY"
                     /locus_tag="ACHOT6_002196"
     CDS             11173..12504
                     /gene="secY"
                     /locus_tag="ACHOT6_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFP9947412.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHOT6_002197"
     CDS             12536..12652
                     /gene="rpmJ"
                     /locus_tag="ACHOT6_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFP9947413.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHOT6_002198"
     CDS             12799..13155
                     /gene="rpsM"
                     /locus_tag="ACHOT6_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFP9947414.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHOT6_002199"
     CDS             13172..13561
                     /gene="rpsK"
                     /locus_tag="ACHOT6_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFP9947415.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHOT6_002200"
     CDS             13595..14215
                     /gene="rpsD"
                     /locus_tag="ACHOT6_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFP9947416.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHOT6_002201"
     CDS             14241..15230
                     /gene="rpoA"
                     /locus_tag="ACHOT6_002201"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFP9947417.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHOT6_002202"
     CDS             15271..15654
                     /gene="rplQ"
                     /locus_tag="ACHOT6_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFP9947418.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15761..16129
                     /locus_tag="ACHOT6_002203"
     CDS             15761..16129
                     /locus_tag="ACHOT6_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFP9947419.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            16140..16565
                     /gene="zntR"
                     /locus_tag="ACHOT6_002204"
     CDS             16140..16565
                     /gene="zntR"
                     /locus_tag="ACHOT6_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFP9947420.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16621..16839
                     /gene="arfA"
                     /locus_tag="ACHOT6_002205"
     CDS             16621..16839
                     /gene="arfA"
                     /locus_tag="ACHOT6_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFP9947421.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHOT6_002206"
     CDS             complement(16836..17246)
                     /gene="mscL"
                     /locus_tag="ACHOT6_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFP9947422.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHOT6_002207"
     CDS             complement(17376..18752)
                     /gene="trkA"
                     /locus_tag="ACHOT6_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFP9947423.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHOT6_002208"
     CDS             complement(18774..20063)
                     /gene="rsmB"
                     /locus_tag="ACHOT6_002208"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFP9947424.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPETQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHOT6_002209"
     CDS             complement(20109..21056)
                     /gene="fmt"
                     /locus_tag="ACHOT6_002209"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFP9947425.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHOT6_002210"
     CDS             complement(21071..21580)
                     /gene="def"
                     /locus_tag="ACHOT6_002210"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFP9947426.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21710..22834
                     /gene="dprA"
                     /locus_tag="ACHOT6_002211"
     CDS             21710..22834
                     /gene="dprA"
                     /locus_tag="ACHOT6_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFP9947427.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22806..23279
                     /gene="smg"
                     /locus_tag="ACHOT6_002212"
     CDS             22806..23279
                     /gene="smg"
                     /locus_tag="ACHOT6_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFP9947428.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23308..23850
                     /locus_tag="ACHOT6_002213"
     CDS             23308..23850
                     /locus_tag="ACHOT6_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFP9947429.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHOT6_002214"
     CDS             23855..24427
                     /gene="tsaC"
                     /locus_tag="ACHOT6_002214"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFP9947430.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24432..25250
                     /gene="aroE"
                     /locus_tag="ACHOT6_002215"
     CDS             24432..25250
                     /gene="aroE"
                     /locus_tag="ACHOT6_002215"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFP9947431.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25247..25504
                     /locus_tag="ACHOT6_002216"
     CDS             25247..25504
                     /locus_tag="ACHOT6_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFP9947432.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25480..26034)
                     /locus_tag="ACHOT6_002217"
     CDS             complement(25480..26034)
                     /locus_tag="ACHOT6_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFP9947433.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6768 a         6257 c         6828 g         6496 t
ORIGIN      
        1 cgtcatactc cgttcttcaa aggctaccgt ccgcagttct acttccgtac tactgacgtg
       61 actggtacca tcgaactgcc ggaaggcgta gagatggtaa tgccgggcga caacatcaaa
      121 atggttgtta ccctgatcca cccgatcgcg atggacgacg gtctgcgttt cgcaatccgt
      181 gaaggcggcc gtaccgttgg cgcgggcgtt gttgctaaag ttctgggcta attacacgtt
      241 aattagtttt gaattgaaaa gggcgcttcg gcgccctttt tgctgcccgt aatttgagct
      301 cgaaatattt agtcgtaatt ttgctcgctg aactatggcg caaataaaac tgtgctattg
      361 taatcataac cattctcatt tacactttgc gcagaatttg aacgggagca atcatgtacg
      421 tttgtttgtg taatggtgta agtgacaaaa aaattcgcca ggcagtccgt cagtttcatc
      481 cgcaatcttt ccaacaattg cgtaagttta ttcctgtggg aaatcaatgc ggtaagtgca
      541 ttcgcgccgc gcgcgaagtg atgcaggacg agttgatgca gatgccagaa tttaaagaga
      601 ttgcctgaag accacgctta ttttttgaca tcccgctagc cccatctacg ctctaatagt
      661 ggaagcggag ggactataaa atgaaaggtg atgttaaaat cataaattat ctcaataaac
      721 tattgggaaa tgagcttgtc gcaatcaatc agtattttct ccatgcccgg atgtttaaaa
      781 actggggcct gatgcgtctc aatgacgtcg aataccatga atccatcgat gagatgaaac
      841 acgctgataa atatatcgag cgtattttgt ttttagaagg aatccccaac ttacaggatt
      901 taggcaaact ggggattggt gaagatgtcg aggagatgct gcaatccgac ctgcgattag
      961 aactggaagg cgcgaaggat ctccgtgagg ctatcgcgta tgccgacagc gttcatgact
     1021 atgttagccg tgatatgatg atcgagatcc ttgccgatga agaaggtcat attgactggc
     1081 tcgaaactga gctggatctc atcggtaaaa tcggtttaca aaattacctg caatcacaaa
     1141 taaaagttaa agattaacgg cctgctagca aactatccca acctacaacg aaacccgcag
     1201 ccgccagaaa tggcccaaac ggcagcgggt tttttaatga ctcttttcct ctcatgagca
     1261 gaccaacaac tacggctccg caagtgaatg aggcagcaag gaaaaccagt cgtggcaaga
     1321 aggtccaggt atgccaggca ccaagggcgg cgagaaactt cacatcacca tagcctaatc
     1381 cttctttatg acgcagtatg cgataccccc agtaaatgac agcgaatgtg ccgtaaccaa
     1441 taatcgcccc ccataacgca tcggctaaac cgtcaggatt acaaacctga gagaacaaaa
     1501 gacctgacca gagtaacggg caggtaaatc tgtcaggtaa caagccatgc tttgcatccc
     1561 ataaaaaaag taggacagat aaacaagcgt acaaaatcag aaagggaagg gtagcgtcca
     1621 taaaacatcc tctgtaattg gagtatcacc atcgaataac tccggatgct cagcaacaga
     1681 gaaatcgctg atttgttaac ctggttccgg tgaaaaacgc tatcttcaca accttacaaa
     1741 tttcgtggaa tacagctcgc aatctgcgcg ttgcgattaa tttctacccg atgcttgcgt
     1801 caggctcaga tttgcgtata atgcgcgggc ttgtcgtagt tgacagcagg ttcaatctga
     1861 acctcagtta gccgaatttg gctacctaac aatgctccca atcggggagc tacgtaagaa
     1921 cggttacact ctcccatcaa tcgtaatggg tctgaggagt aatcattttc gtttataaaa
     1981 taattggagc tctggtctca tgcagaacca aagaatccgt atccgcctga aagcgtttga
     2041 tcatcgtctg atcgatcaag caaccgcgga aatcgtcgag actgccaagc gcactggtgc
     2101 gcaggtccgt ggtccgatcc cgctgccgac acgcaaagag cgcttcactg ttctgatctc
     2161 cccgcacgtc aacaaagacg cgcgcgatca gtacgaaatc cgtactcact tgcgtctggt
     2221 tgacatcgtt gagccaaccg agaaaaccgt tgatgctctg atgcgtctgg atctggctgc
     2281 cggtgtagac gtgcagatca gcctgggtta atcaggtcat tgagcgattg agaggttgaa
     2341 acaatgattg gtttagtcgg taaaaaagtg ggtatgaccc gtatcttcac agaagacggc
     2401 gtttctatcc cagtaaccgt aatcgaagtt gaagcaaacc gcgttactca ggttaaagac
     2461 ctggctaacg atggctaccg tgctattcag gtgaccaccg gtgctaaaaa agctaaccgt
     2521 gtgaccaagc ctgaagctgg ccacttcgct aaagctggcg tagaagctgg ccgtggtctg
     2581 tgggaattcc gcctggctga aggcgaagag ttcactgtag gtcagagcat tagcgttgaa
     2641 ctgtttgctg acgttaaaaa agttgacgta actggcacct ctaaaggtaa aggtttcgca
     2701 ggtaccgtta agcgctggaa cttccgtacc caggacgcta ctcacggtaa ctccttgtct
     2761 caccgcgttc cgggttctat cggtcagaac cagactccgg gcaaagtgtt caaaggcaag
     2821 aaaatggcag gtcagatggg taacgaacgt gtaaccgttc agagccttga cgtagtacgc
     2881 gttgacgctg agcgcaacct gctgctggtt aaaggtgctg tcccgggtgc aaccggtagc
     2941 gacctgatcg ttaaaccagc tgtgaaggcg taaggagata gcaatggaat tagtattgaa
     3001 agacgcgcag agcgcgctga ctgtttccga aactaccttc ggtcgtgatt tcaacgaagc
     3061 gctggttcac caggttgttg ttgcttatgc agctggtgct cgtcagggta ctcgtgctca
     3121 gaagactcgt gctgaagtaa ctggttccgg taaaaaaccg tggcgccaga aaggcaccgg
     3181 ccgtgcgcgt tctggttcta tcaagagccc gatctggcgt tctggtggcg tgacctttgc
     3241 tgctcgtccg caggaccaca gtcaaaaagt taacaagaag atgtaccgcg gcgcgctgaa
     3301 aagcatcctg tccgaactgg tacgtcagga tcgtctgatc gttgtcgaga agttctctgt
     3361 agaagcgccg aaaactaagc tgctggcaca gaaactgaaa gacatggctc tggaagatgt
     3421 gctgatcatc accggtgagc tggacgaaaa cctgttcctg gctgcgcgca acctgcacaa
     3481 ggttgacgta cgcgatgcaa ctggtatcga cccggttagc ctgatcgcct tcgacaaagt
     3541 cgtaatgact gctgatgctg ttaagcaagt tgaggagatg ctggcatgat tcgtgaagaa
     3601 cgtctgctga aggtgctgcg tgcaccgcac gtttctgaaa aagcgtctac tgcgatggaa
     3661 aaatccaaca ccatcgtact caaagttgct aaagacgcga ccaaagcaga aatcaaagct
     3721 gctgtgcaga aactgtttga agtcgaagtc gaagtcgtta acaccctggt agttaaaggg
     3781 aaagttaaac gtcacggaca gcgtatcggt cgtcgtagcg actggaaaaa agcttacgtc
     3841 accctgaaag aaggccagaa tctggacttc gttggcggcg ctgagtaagt cggaggagta
     3901 atacaatggc agttgttaaa tgtaaaccga catctccggg tcgtcgccac gtagttaaag
     3961 tggttaaccc tgagctgcac aagggcaaac cttttgctcc gttgctggaa aaaaacagca
     4021 aatccggtgg tcgtaacaac aatggccgta tcaccactcg tcatatcggt ggtggccaca
     4081 agcaggctta ccgtattgtt gacttcaaac gcaacaaaga cggtatcccg gcagttgttg
     4141 aacgtcttga gtacgatccg aaccgttccg cgaacatcgc gctggttctg tacaaagacg
     4201 gtgaacgccg ttacatcctg gcccctaaag gcctgaaagc tggcgaccag attcagtctg
     4261 gcgttgatgc tgcaatcaaa ccaggtaaca ccctgccgat gcgcaacatc ccggttggtt
     4321 ctactgttca taacgtagaa atgaaaccag gtaaaggcgg tcagctggca cgttccgctg
     4381 gtacttacgt tcagatcgtt gctcgtgatg gtgcttatgt caccctgcgt ctgcgttctg
     4441 gtgaaatgcg taaagtagaa gcagactgcc gtgcaactct gggcgaagtt ggcaatgctg
     4501 agcatatgct gcgcgttctg ggtaaagcag gtgctgcacg ctggcgtggt gttcgtccga
     4561 ccgttcgcgg taccgcgatg aacccggtag accacccaca tggtggtggt gaaggtcgta
     4621 actttggtaa gcacccggta actccgtggg gcgttcagac caaaggtaag aagacccgca
     4681 gcaacaagcg tactgataaa ttcatcgtac gtcgccgtag caaataattt tagaggataa
     4741 gccatgccac gttctctcaa gaaaggtcct tttattgacc tgcacttgct gaagaaggta
     4801 gagaaagcgg tggaaagcgg agacaagaag cccctgcgca cttggtcccg tcgttcaacg
     4861 atctttccta acatgatcgg tttgaccatc gctgtccata atggtcgtca gcacgttccg
     4921 gtatttgtaa ccgacgaaat ggtcggtcac aaactgggtg aattcgcacc gactcgtact
     4981 tatcgcggcc acgctgctga taaaaaagcg aagaagaaat aaggtaggag gaagagatgg
     5041 aaactatcgc taaacatcgc catgctcgtt cttctgctca gaaggttcgc cttgttgctg
     5101 acctgattcg cggtaagaaa gtgtcgcagg ctctggatat tttgacctac accaacaaga
     5161 aagcggctgt actggtcaag aaagttctgg aatctgccat tgctaacgct gaacacaacg
     5221 atggcgctga cattgacgat ctgaaagtta cgaaaatttt cgtagacgaa ggcccgagca
     5281 tgaagcgcat tatgccgcgt gcaaaaggtc gtgcagatcg catcctgaag cgcaccagcc
     5341 acatcactgt ggttgtgtcc gatcgctgag actctggaga ctagcaatgg gtcagaaagt
     5401 acatcctaat ggtattcgcc tgggtattgt aaaaccatgg aactctacct ggtttgcgaa
     5461 caccaaagaa ttcgctgaca acctggacag cgattttaaa gtacgtcagt acctgactaa
     5521 ggaactggct aaagcgtccg tatctcgtat cgttatcgag cgtccggcta agagcatccg
     5581 tgtaaccatt cacactgctc gcccgggtat cgttatcggt aaaaaaggtg aagacgtaga
     5641 aaaactgcgt aaggtcgtag cggacatcgc tggcgttcct gcacagatca acatcgccga
     5701 agttcgtaag cctgaactgg acgcaaaact ggttgctgac agcatcactt ctcagctgga
     5761 acgtcgcgtt atgttccgtc gtgctatgaa gcgtgctgta cagaacgcaa tgcgtctggg
     5821 cgctaaaggt attaaagttg aagttagcgg ccgtctgggc ggcgcggaaa tcgcacgtac
     5881 cgaatggtac cgcgaaggtc gcgtaccgct gcacactctg cgtgctgaca tcgactacaa
     5941 cacctctgaa gcgcacacca cttacggtgt aatcggcgtt aaagtgtgga tcttcaaagg
     6001 cgagatcctg ggtggtatgg ctgctgttga acaaccggaa aaaccggctg ctcagcctaa
     6061 aaagcagcag cgtaaaggcc gtaaataagg agcgtcgctg atgttacaac caaagcgtac
     6121 aaaattccgt aaaatgcaca aaggccgtaa ccgcggtctg gcgcagggta cggatgttag
     6181 cttcggcagc ttcggtctga aagctgttgg ccgtggtcgt ctgactgccc gtcagatcga
     6241 agcagcacgt cgtgctatga cccgtgcagt taagcgtcaa ggtaagatct ggatccgtgt
     6301 gttcccggac aaaccgatca ctgaaaagcc gctggcagtg cgtatgggta aaggtaaagg
     6361 taacgtggag tattgggttg ccttgattca gccgggtaaa gtcctgtatg aaatggacgg
     6421 tgttccggaa gagctggccc gtgaagcatt caagctggca gcagcgaaac tgccgattaa
     6481 aaccaccttt gtaactaaga cggtgatgta atgaaagcaa aagagctgcg tgagaagagc
     6541 gttgaagagc tgaacaccga gctgctgaac ctgctgcgtg agcagttcaa cctgcgtatg
     6601 caggctgcaa gtggccagct gcaacagtct cacctgttga agcaagtgcg tcgcgatgtc
     6661 gcacgcgtta agactttact gaacgagaag gcgggtgcgt aatgaccgat aaaatccgta
     6721 ctctgcaagg tcgcgttgtt agcgacaaaa tggagaaatc cattgttgtt gctatcgaac
     6781 gttttgtgaa acacccgatc tacggtaaat tcatcaagcg tacgaccaaa ctgcacgtac
     6841 atgacgagaa caacgaatgc ggtatcggtg acgtggttga aatccgcgaa tgccgtccgc
     6901 tgtccaagac taaatcctgg acgctggttc gcgttgtaga gaaagcggtt ctgtaataca
     6961 gtacactctc tcgatacgaa taaacggctc agaaatgagc cgtttatttt ttctacccat
     7021 atccttgaag cggtgttata atgccgcgcc ctcgatatgg ggatttttaa cgacctgatt
     7081 ttcgggtctc agtagtagtt gacattagcg gagcactaaa atgatccaag aacagactat
     7141 gctgaacgtc gccgacaact ccggtgcacg tcgcgtaatg tgtatcaagg ttctgggtgg
     7201 ctcgcaccgt cgctacgcag gcgtaggcga catcatcaag atcaccatca aagaagcaat
     7261 tccgcgtggt aaggtcaaaa aaggtgatgt gctgaaggcg gtagtggtgc gcaccaagaa
     7321 gggtgttcgt cgcccggacg gttctgtcat tcgctttgat ggtaatgctt gtgttcttct
     7381 gaacaacaac agcgagcagc ctatcggtac gcgtattttt gggccggtaa ctcgtgagct
     7441 tcgtagtgag aagttcatga aaattatctc tctggcacca gaagtactct aaggagcgaa
     7501 tcatggcagc gaaaatccgt cgtgatgacg aagttatcgt gttaaccggt aaagataaag
     7561 gtaaacgcgg taaagttaag aatgtcctgt cttccggcaa ggtcattgtt gaaggtatca
     7621 acctggttaa gaaacatcag aagccggttc cggccctgaa ccaaccgggt ggcatcgttg
     7681 aaaaagaagc cgctattcag gtttccaacg tagcaatctt caatgcggca accggcaagg
     7741 ctgaccgtgt aggctttaga ttcgaagacg gcaaaaaagt ccgtttcttc aagtctaaca
     7801 gcgaaactat caagtaattt ggagtagtac gatggcgaaa ctgcatgatt actacaaaga
     7861 cgaagtagtt aaaaaactca tgactgagtt taactacaat tctgtcatgc aagtccctcg
     7921 ggtcgagaag atcaccctga acatgggtgt tggtgaagcg atcgctgaca aaaaactgct
     7981 ggataacgca gcagcagacc tggcagcaat ctccggtcaa aaaccgctga tcaccaaagc
     8041 acgcaaatct gttgcaggct tcaaaatccg tcagggctat ccgatcggct gtaaagtaac
     8101 tctgcgtggc gaacgcatgt gggagttctt tgagcgcctg atcactattg ctgtacctcg
     8161 tatccgtgac ttccgtggcc tgtccgctaa gtctttcgac ggtcgtggta actacagcat
     8221 gggtgtccgt gagcagatca tcttcccaga aatcgactac gataaagtcg accgcgttcg
     8281 tggtttggat attaccatta ccactactgc gaaatctgac gaagaaggcc gcgctctgct
     8341 ggctgccttt gacttcccgt tccgcaagta aggtagggtt actaaatggc taagcaatca
     8401 atgaaagcac gcgaagtaaa acgcgtagct ttagctgata aatacttcgc gaaacgcgct
     8461 gaactgaaag cgatcatctc tgatgtgaac gcttccgacg aagatcgttg gaacgctgtt
     8521 ctcaagctgc agactctgcc gcgtgattcc agcccgtctc gtcagcgtaa ccgctgccgt
     8581 caaacaggtc gtccgcatgg tttcctgcgg aagttcgggt tgagccgtat taaggtccgt
     8641 gaagccgcta tgcgcggtga aatcccgggt ctgaaaaagg ctagctggta attgtcacca
     8701 attgaatcac gggagtaaag acagatgagc atgcaagatc cgatcgcgga tatgctgacc
     8761 cgtatccgta acggtcaggc cgcgaacaaa gctgcggtca ccatgccttc ctccaagctg
     8821 aaagtggcaa tcgccaacgt gctgaaggaa gaaggtttta ttgaagattt taaagttgaa
     8881 ggcgacacca agcctgaact ggaacttact ctgaagtatt tccagggcaa agctgttgta
     8941 gaaagcattc agcgtgtcag ccgcccaggt ctgcgcatct ataaacgtaa agatgagctg
     9001 ccgaaagtta tggcgggtct gggtatcgca gttgtttcta cctctaaagg tgttatgact
     9061 gatcgtgcag cgcgccaggc tggtcttggt ggcgaaatta tctgctacgt agcctaatcg
     9121 gaggaaaaaa tgtctcgtgt tgctaaagca ccggtcgttg ttcctgccgg cgttgacgta
     9181 aaaatcaacg gtcaggttat tacgatcaaa ggtaaaaacg gcgagctgac tcgtactctc
     9241 aacgatgctg ttgaagttaa acatgcagat aataccctga ccttcggtcc gcgtgatggt
     9301 tacgcagacg gttgggcaca ggctggtacc gcgcgtgccc tgctgaactc aatggttatc
     9361 ggtgttaccg aaggcttcac taagaagctg cagctggttg gtgtaggtta ccgtgcagcg
     9421 gttaaaggta atgtgattaa cctgtctctg ggtttctctc atcctgttga ccatcagctg
     9481 cctgcgggta tcactgctga atgtccgact cagactgaaa tcgtgctgaa aggcgctgat
     9541 aagcaggtga tcggccaggt tgcagcggat ctgcgcgcct accgtcgtcc tgagccttat
     9601 aaaggcaagg gtgttcgtta cgccgacgaa gtcgtgcgta ccaaagaggc taagaagaag
     9661 taaggtaaca ctatggataa gaaatctgct cgtatccgtc gtgcgacccg cgcacgccgc
     9721 aagctccagg agctgggcgc aactcgcctg gtggtacatc gtaccccgcg tcacatttac
     9781 gcacaggtaa ttgcaccgaa cggttctgaa gttctggtag ctgcttctac tgtagaaaaa
     9841 gctatcgctg aacaactgaa gtacaccggt aacaaagacg cggctgcagc tgtgggtaaa
     9901 gctgtcgctg aacgcgctct ggaaaaaggc atcaaagatg tatcctttga ccgttccggg
     9961 ttccaatatc atggtcgtgt ccaggcactg gcagatgctg cccgtgaagc tggccttcag
    10021 ttctaaggta gaggtgtaag atggctcaca tcgaaaaaca agctggcgaa ctgcaggaaa
    10081 agctgatcgc ggtaaaccgc gtatctaaaa ccgttaaagg tggtcgtatt ttctccttca
    10141 cagctctgac tgtagttggc gatggtaacg gtcgcgttgg ttttggttac ggtaaagcgc
    10201 gtgaagttcc agcagcgatc cagaaagcga tggaaaaagc ccgtcgcaat atgattaacg
    10261 tcgcgctgaa taacggcact ctgcaacacc ctgttaaagg tgttcacacg ggttctcgcg
    10321 tattcatgca gccggcttcc gaaggtaccg gtatcatcgc cggtggtgca atgcgcgccg
    10381 ttctggaagt cgctggggtt cataacgttc tggctaaagc atatggttcc accaacccga
    10441 tcaacgtggt tcgtgcaact attgatggcc tggaaaatat gaattctcca gaaatggtcg
    10501 ctgccaagcg tggtaaatcc gttgaagaaa ttctggggaa ataaaccatg gcaaagacta
    10561 ttaaaattac tcaaacccgc agtgcaatcg gtcgtctgcc gaaacacaag gcaacgctgc
    10621 ttggcctggg tctgcgtcgt attggtcaca ccgtagagcg cgaggatact cctgctattc
    10681 gcggtatgat caacgcggtt tccttcatgg ttaaagttga ggagtaagag atgcgtttaa
    10741 atactctgtc tccggccgaa ggctccaaaa aggcgggtaa acgcctgggt cgtggtatcg
    10801 gttctggcct cggtaaaacc ggtggtcgtg gtcacaaagg tcagaagtct cgttctggcg
    10861 gtggcgtacg tcgcggtttc gagggtggtc agatgcctct gtaccgtcgt ctgccgaaat
    10921 tcggcttcac ttctcgtaaa gcagcgatta cagccgaagt tcgtctgtct gacctggcta
    10981 aagtagaagg cggtgttgta gacctgaaca cgctgaaagc ggctaacatt atcggtatcc
    11041 agatcgagtt cgcgaaagtg atcctggctg gcgaagtaac gactccggta actgttcgtg
    11101 gcctgcgtgt tactaaaggc gctcgtgctg ctatcgaagt tgctggcggt aaaatcgagg
    11161 aataagtagc agatggctaa acaaccggga ttagattttc aaagtgccaa aggtggctta
    11221 ggcgagctga aacgcagact gctgtttgtt atcggtgcgc tgattgtgtt tcgtattggc
    11281 tcttttattc cgatccctgg tattgatgcc gctgtacttg ccaaactgct tgagcaacag
    11341 cgaggcacca tcattgagat gtttaacatg ttctctggtg gtgctctcag ccgtgcttct
    11401 atctttgctc tggggatcat gccgtatatt tcggcgtcga tcattatcca gctgctgacg
    11461 gtggttcacc caacgttggc agaaattaag aaagaagggg agtctggtcg tcgtaagatc
    11521 agccagtaca cccgctacgg tactctggtg ctggcaatat tccagtcgat cggtattgct
    11581 accggtctgc cgaatatgcc tggtatgcaa ggcctggtga ttaacccggg ctttgcattc
    11641 tacttcaccg ctgttgtaag tctggtcaca ggaacgatgt tcctgatgtg gttgggcgaa
    11701 cagatcactg aacgaggtat cggcaacggt atttcaatca ttatcttcgc cggtattgtc
    11761 gcgggactcc cgccagccat tgcccatact atcgagcaag cgcgtcaagg cgacctgcac
    11821 ttcctcgtgt tgctgttggt tgcagtatta gtatttgcag tgacgttctt tgttgtattt
    11881 gttgagcgtg gtcaacgccg cattgtggta aactacgcga aacgtcagca aggtcgtcgt
    11941 gtctatgctg cacagagcac acatttaccg ctgaaagtga atatggcggg ggtaatcccg
    12001 gcaatcttcg cttccagtat tattctgttc ccggcgacca tcgcgtcatg gttcgggggc
    12061 ggtactggtt ggaactggct gacaacaatt tcgctgtatt tgcagcctgg gcaaccgctt
    12121 tatgtgttac tctatgcgtc tgcaatcatc ttcttctgtt tcttctacac ggcgttggtt
    12181 ttcaacccgc gtgaaacagc agataacctg aagaagtccg gtgcatttgt accaggaatt
    12241 cgtccgggag agcaaacggc gaagtatatc gataaagtaa tgacccgcct gaccctggtt
    12301 ggtgcgctgt atattacctt tatctgcctg atcccggagt tcatgcgtga tgcaatgaaa
    12361 gtaccgttct acttcggtgg gacctcactg cttatcgttg ttgtcgtgat tatggacttt
    12421 atggctcaag tgcaaactct gatgatgtcc agtcagtatg agtctgcatt gaagaaggcg
    12481 aacctgaaag gctacggccg ataattggtc gcccgagaag ttacggagag taaaaatgaa
    12541 agttcgtgct tccgtcaaga aattatgccg taactgcaaa atcgttaagc gtgatggtgt
    12601 catccgtgtg atttgcagtg ccgagccgaa gcataaacag cgccaaggct gattttttcg
    12661 catatttttc ttgcaaagtt gggttgagct ggctagatta gccagccaat cttttgtatg
    12721 tctgtgcgtt tccatttgag tatcctgaaa acgggctttt cagcatggaa cgtacatatt
    12781 aaatagtagg agtgcatagt ggcccgtata gcaggcatta acattcctga tcataagcat
    12841 gccgtaatcg cattaacttc gatttatggc gtcggcaaga cccgttctaa agccatcctg
    12901 gctgcagcgg gtatcgctga agatgttaag atcagtgagc tgtctgaagg acaaatcgac
    12961 acgctgcgtg acgaagttgc caaatttgtc gttgaaggtg atctgcgccg tgaaatcagc
    13021 atgagcatca agcgcctgat ggatcttggt tgctatcgcg gtttgcgtca tcgtcgtggt
    13081 ctcccggttc gcggtcagcg taccaagacc aacgcacgta cccgtaaggg tccgcgcaaa
    13141 ccgatcaaga aataatcggg gtgattgaat aatggcaaag gcaccaattc gtgcacgtaa
    13201 acgtgtaaga aaacaagtct ctgacggcgt ggctcatatc catgcttctt tcaacaacac
    13261 catcgtgact atcactgatc gtcagggtaa cgcgttgggt tgggcaacag ccggtggttc
    13321 cggtttccgt ggttctcgca aatccactcc gtttgcagct caggttgcag cagagcgttg
    13381 cgctgacgcc gtgaaagaat acggcatcaa gaatctggaa gttatggtta aaggtccggg
    13441 tccaggccgc gaatctacta ttcgtgctct gaacgccgca ggtttccgca tcactaatat
    13501 tactgatgtg actccgatcc ctcataacgg ttgtcgtccg ccgaaaaaac gtcgcgtata
    13561 acgcctcgtt ttccaggttt gttggagaaa gaaaatggca agatatttgg gtcctaagct
    13621 caagctgagc cgtcgtgagg gcaccgactt attccttaag tctggcgttc gcgcgatcga
    13681 taccaagtgt aaaattgaac aagctcctgg ccagcacggt gcgcgtaaac cgcgtctgtc
    13741 tgactatggt gtgcagttgc gtgaaaagca aaaagttcgc cgtatctatg gtgtgctgga
    13801 gcgtcagttc cgtaactact acaaagaagc agcacgtctg aaaggcaaca ccggtgaaaa
    13861 cctgttggct ctgctggaag gtcgtctgga caacgttgta taccgtatgg gcttcggtgc
    13921 cactcgtgca gaagcacgtc agctggttag ccataaagca attatggtaa acggtcgtgt
    13981 tgttaacatc gcttcttatc aggttagtcc gaatgacgtt gtaagcattc gtgagaaagc
    14041 gaagaagcag tctcgcgtga aagccgctct ggagctggct gagcagcgtg aaaagccaac
    14101 ctggctggaa gttgatgctg gcaagatgga aggtacgttt aagcgtaagc cggagcgttc
    14161 tgatctgtct gcggacatta acgaacacct gatcgtcgag ctttactcca agtaaagctt
    14221 agtaccaaag agaggacaca atgcagggtt ctgtgacaga gtttctaaaa ccgcgcctgg
    14281 ttgatatcga gcaagtgagt tcgacgcacg ccaaggtgac ccttgagcct ttagagcgtg
    14341 gctttggcca tactctgggt aacgcactgc gccgtattct gctctcatcg atgccgggtt
    14401 gcgcggtgac cgaggttgag attgatggtg tactacatga gtacagcacc aaagaaggcg
    14461 ttcaggaaga tatcctggaa atcctgctca acctgaaagg gctggcggtg agagttcagg
    14521 gcaaagatga agttattctt accttgaata aatctggcat tggccctgtg actgcagccg
    14581 atatcaccca cgacggtgat gtcgaaatcg tcaagccgca gcacgtgatc tgccacctga
    14641 ccgatgagaa cgcgtctatt agcatgcgta tcaaagttca gcgcggtcgt ggttatgtgc
    14701 cggcttctac ccgaattcat tcggaagaag atgagcgccc aatcggccgt ctgctggtcg
    14761 acgcatgcta cagccctgtg gagcgtattg cctacaatgt tgaagcagcg cgtgtagaac
    14821 agcgtaccga cctggacaag ctggtcatcg aaatggaaac caacggcaca atcgatcctg
    14881 aagaggcgat tcgtcgtgcg gcaaccattc tggctgaaca actggaagct ttcgttgact
    14941 tacgtgatgt acgtcagcct gaagtgaaag aagagaaacc agagttcgat ccgatcctgc
    15001 tgcgccctgt tgacgatctg gaattgactg tccgctctgc taactgcctt aaagcagaag
    15061 ctatccacta tatcggtgat ctggtacagc gtaccgaggt tgagctcctt aaaacgccta
    15121 accttggtaa aaaatctctt actgagatta aagacgtgct ggcttcccgt ggactgtctc
    15181 tgggcatgcg cctggaaaac tggccaccgg caagcatcgc tgacgagtaa ccggatcaca
    15241 ggttaaggtt ttactgagaa ggataaggtc atgcgccatc gtaagagtgg tcgtcaactg
    15301 aaccgcaaca gcagccatcg ccaggctatg ttccgcaata tggcaggttc actggttcgt
    15361 catgaaatca tcaagacgac tctgcctaaa gcgaaagagc tgcgccgcgt agttgagccg
    15421 ctgattactc ttgccaagac tgatagcgtt gctaatcgtc gtctggcatt cgcccgtact
    15481 cgtgataacg agatcgtggc aaaactgttt aacgaactgg gcccgcgttt cgcgagccgt
    15541 gccggtggtt acactcgtat tctgaagtgt ggcttccgtg caggcgacaa cgcgccgatg
    15601 gcttacatcg agctggttga tcgttcagag aaagcagaag ctgctgcaga gtaatctgaa
    15661 gcaacgtaaa aaaacccgcc ccggcgggtt tttttatacc cgtagtatcc ccacttacct
    15721 acaatagctg tactcttttt gttcatcccc tggagtattt atgtggttac ttgaccagtg
    15781 ggcagagcgc catatagcag aagcgcaagc gaaaggtgag tttgataacc tggcaggtag
    15841 cggcgaacca ttgatactgg atgatgattc tcacgtgcca ccggaattac gtgcggggta
    15901 tcgcttgctg aagaatgccg gttgcttacc gccagaactt gagcaacgga gagaagcaat
    15961 tcagcttctg gatattctca aaggtatccg tcacgatgat ccgcaatatc aagaggttag
    16021 ccgtcgattg tcattactgg aattgaagct gcgacaagct ggattgagta ccgatttttt
    16081 acgcggcgat tatgctgaca agttgttgga caaaatcaac gataactagt ggagtatgta
    16141 tgtatcgcat tggtgagctg gcaaaaatgg cggaagtaac acctgacacg attcgttatt
    16201 acgaaaaaca gcagatgatg gagcatgaag tgcgtactga aggcgggttt cgcctgtata
    16261 ccgaaagcga tctccagcga ttgaaattta tccgccatgc cagacaacta ggtttcagtc
    16321 tggagtcgat ccgcgagttg ctgtcgatcc gcatcgatcc tgaacaccat acctgtcagg
    16381 agtcaaaagg cattgtgcag gaaagattgc aggaagtcga agcacggata gccgagttgc
    16441 agagtatgca gcgttccttg caacgcctta acgatgcctg ttgtgggacc gcccatagca
    16501 gtgtttattg ttcgattctt gaagctcttg aacaaggggc gagtggcgtt aagagtggct
    16561 gttgattttt tgcactggca ggattacact cgcgccgtaa aataaccaac tggagttttt
    16621 atgagtcgat atcagcatac taaagggcag ataaaggata atgcgataga agcattacta
    16681 catgatccct tattccgaca gcgcgtagag aaaaataaga aggggaaagg cagttacatg
    16741 cgaaaaggaa aacatggcaa tcgggggaac tgggaggcca gtggcaagaa agtaaatcac
    16801 ttttttacca ctggtcttct gctttcagga gcttgttaag agcggttatt ctgctctttc
    16861 agcaaatcac gaatttctgt cagtaatact tcttctttag ttggtgcagg tgcggctgct
    16921 ggttcttctt ttttccgatt cagtttgttg attagcttaa tcgccataaa gatggcaaag
    16981 gccacaatca gaaaatcaaa gacgttttga atgaagacac cgtaatgcat cacaacagca
    17041 gggatatccc cctgcgcatc gcgtagcgtg acagcaaact gtttaaagtc gatcccgcca
    17101 attaataagc ccagaggtgg catgatgata tcggcaacca gtgaagatac aatcttcccg
    17161 aatgccgcac cgataatgac acccaccgcc aaatccacca cgttcccgcg catcgcaaat
    17221 tcgcgaaatt ctttaataat gctcatgtta ttctccctat gaagccgaca accataagtc
    17281 taacaaatgt taagccattt tcctgccagg aatgaattta attaatcctt gataatcaaa
    17341 gggaaataat aaggcgtcat tagacgcctt attaattaca agaagaaagg gcttggctgg
    17401 aagagtcttt cgacgtcggt aataaacttt ttatctgtga ggaacataat tacatgatcg
    17461 ccttgctcaa tgcgcagatt gtcattggca atcatcacgt cgtttccacg taccaccgct
    17521 ccaataatcg ttcctggcgg tagcttgatt tcgtcaatga ctctgccgac aacgcgtgag
    17581 gtgctttcat caccgtgagc aacggcttca atagcttctg ctacgccgcg gcgcaacgag
    17641 gaaacaccaa caatatctgc ttttcgcaca tggctaagca acgcagaaat agttgcttgt
    17701 tgtggtgaaa tcgcaatatc gatcacactc ccctgaacca gatccacata agcgcgacgc
    17761 tggatcaata ccatcacctt tttcgcaccc atacgtttgg caagcatggc ggacatgata
    17821 ttggcctcgt catcgttggt gacggcaata aacagatcaa cttgatcgat atgttcttca
    17881 gccagcagtt cttgatccga cgcatcacca aaaaagacga tcgtattctg caacttttcc
    17941 gccagttcgg cagcgcgctg ctgattacgt tcgatgagtt taacgctgta atctttttcc
    18001 agacgacgcg ccagccctgc accgatatta ccgccaccaa ccagcatgat ccgcttatac
    18061 ggtttttcca gtcgctgtaa ttcactcatc accgcgcgga tatgctgtga agcggcaata
    18121 aagaacactt catcaccagc ttcaacaatg gtcgaacctt gcggacgaat ggggcgatcg
    18181 tggcggaaaa ttgctgccac acgagtatcg atatgtggca tatgttcgcg catggtcgaa
    18241 agtgcattac caatcagcgg gccgccataa taggctttaa ccacagccag gctgacttta
    18301 ccctcagcga agtttaccac ctgcaatgcg ccgggatact caatcagtcg gtaaatatta
    18361 tcgataacca actgctctgg tgcgatcaga tgatcaatcg gtacagcatc tgaatgaaat
    18421 agcttatcgg catcgcgcac gtagtctggt gagcggatgc gagcgatgcg attaggggtg
    18481 ttgaaaagcg agtaggctac ctggcaggca accatattgg tttcatctga actggttaca
    18541 gcaaccagca tatcggcgtc gtcggcacct gcctcccgca atacgcgtgg atgagagcca
    18601 tgcccctgca cgacccgcag gtcaaattta tcctgtaagg tccgcagacg ctcaccgttg
    18661 gtatcgacaa cagtaatatc gttgttctcg ccaaccaggt tttccgccag tgtgccgcca
    18721 acctggccgg cacccagaat gataattttc atcagtcgcg acccgttatc tcatcacttt
    18781 ttgattagct tagcgtaaaa gaagccgtcg ccctcttcgg caccaggtag attttgttta
    18841 cccggttgct ctggtgttcc tgtttcacaa agttctgcat cagcggtacg ttgcaaaaaa
    18901 gctttaatct gcaggctatt ttcttccggt aacaccgaac aggtggcata gaccagggtt
    18961 ccaccggatt ttaaatgcgg ccaaatggcg tcgagaattt cagactgcaa ttgcgcgagt
    19021 tccgggatat cgcgatcgcg acgtaaccat ttaatatccg gatggcgacg aatcacaccg
    19081 gttgctgaac aaggcgcatc taataaaatg cgatcaaact gttgctcgcc acaccattgg
    19141 gaagggtagc ggccatcacc ttgtttcacg gtcgccttca taccaaggcg ttttaaattg
    19201 tcgtaaacgc gagagaggcg ctgttcgtca atatcaaccg caacaacctg cgtttctggt
    19261 gccacctcaa ggatatgcgt tgttttaccg ccgggggcgg cacaaagatc caaaatgtgt
    19321 tcaccgtttt gtggcgcaag ccaggtcatg caaccttgtg ctgatgcatc ctgaacggta
    19381 acccatccgt cttcaaaacc aggtagcgca tgaacaggtg caggtgtttc cagacgtaca
    19441 gcatcagggt aatccgcatg cgggaaacct ttcattcctg cttcatccag caatgcaagc
    19501 cagctgtcgc gggaatgatg cgtacgatta acgcgcagcc acattggcgg acgctggtta
    19561 ttggcttcga cgatggattg ccactgctct ggatacgctt tttgcagacg cttcagcaac
    19621 caggaaggat gcagataacg tgcatcactg gcattaaact cggctaataa ctcttcttgc
    19681 tgacgctgga actggcgtaa tacaccgttt atcaaccctt taagttgcgg acgcttaatt
    19741 gcgatagcgc cttcaaccgt ttcagccagc gcagcatgag gtggaatgcg ggtataaagc
    19801 agttgataca aaccaaccat aatcaggtaa tgcacggtcc gctgtttgcc ggtcatcgga
    19861 cgggccatta acttattaat cagccagtct aactgcgaaa gcgtacgcag tacgccaaag
    19921 cacaactctt gaagaagtgc tttgtcttta tcggaaactt tttgctggag cggtggcaga
    19981 atgttgctta atgattgccc ttgctcgacg acttgttcaa cggcctgggc cgccatgcta
    20041 cgtaaattac gttgtttttt cataagtata aaaatgcccg gcaagaccgg gcttagaaga
    20101 gtggactatc agaccagacg gttgcccgga acaaaccatt cccgacgaga gttcaggagg
    20161 tcttgcgcgc tcatcgcttt cttacccgca ggttgtaacg agagcaggtt caggatgcca
    20221 tcaccagtcg caacctgaat gccttgttta ttggcttcaa ggatcgttcc tggtgcagcg
    20281 ttggttgccg tatcaatgac cgacgctttc cagactttaa ccggttgtcc ttcaatttcc
    20341 agccagctca ttggccatgg gttgaaagcg cgaatgcagc gttcaagctg cgctgccgaa
    20401 agcgaccagt caatacgcgc ttcttcttta ctcaacttct cggcgtaagt gacaagagtt
    20461 tcgtcctgaa cttctggttt cgccgtgccg tctgccagtt gtttcaacgt ggtgataagc
    20521 ccttgtgggc caagctctgc cagcttgtcg tacagcgtac cactggtatc ttctgcagta
    20581 atcgggcagg agagcttata gagcatgtca ccggtgtcta aaccaacatc catttgcata
    20641 atggtcacac cagtttctgc atcacccgcc catagtgagc gttggattgg tgcagcaccg
    20701 cgccagcgtg gcagcagtga accatgaacg ttgatacagc caagacgcgg catctccagc
    20761 acagctttag gcagaattaa accataggcg acgacgacca taacatcagc ctgcagatcg
    20821 gcgaccagtt gctggttttc ttgtggacgc agggaaacag gttgaaaaac gggcagacct
    20881 ttttcctcag ccagaacttt aatcgggctg ggcatcagtt ttttaccgcg tcctgccggt
    20941 cggtctggct gggtgaacac gccaacgacg ttatgaccag aagacaacag cgcgtcgaga
    21001 tgacgcgctg caaagtcagg tgtacccgca aaaataatac gtagtgattc tgacacgtta
    21061 gttcttatcc ttaagcccgg gctttcagac gatccagttt ttcaactttc tgacgaatac
    21121 gttgttgttt cagcggtgac agataatcca taaacagttt gccgaccagg tgatccatct
    21181 catgctgaat acagatggct aacagaccat ctgcctccag ttcaaatggt ttaccgtcgc
    21241 ggtccagggc gcgaatttta actttctctg cgcgtggcac taaagcacgt tgttcaggga
    21301 tcgacaggca accttcttca atgcctgttt cgccgctttt ttctaaaagc tccggattga
    21361 ttaacaccag ccgttcgtca cggttttccg aaacatcaat aacaatgata cgttgatgga
    21421 tatcaacctg ggttgccgcc aggccaatac cttcttctgc atacatcgtc tcgaacatat
    21481 catcgacgat acgctgaatt tctgcattca cttcttctac cggtttagca actttgcgaa
    21541 gccgctcgtc cggaatatgt aacacttgca aaactgacat aaatctccag agatgtgttc
    21601 aggagttaga aagattattt cttctattct agacaaatcc ccctctgatt gacagcatca
    21661 ctgaccaatc gcaaagattg ctaaggctgc ttatggcagg gagataagga tggtcgatac
    21721 agatatttgg ctgcgtttaa tgagcatcag cagcttgtac ggcgatgata tggtccgtat
    21781 agctcactgg ctggcaaaac agtcgcatat tgatgcggtt gtattgcagc aaacagggct
    21841 tacattgcgg caggcacaac gctttctttc atttccgcga aagagtatcg aaagctcact
    21901 ttgttggttg gagcaaccca accatcattt aatccctgcg gacagcgaat tttatcctcc
    21961 tcaacttctg gtgacgacag attaccccgg cgcactgttt gttgaaggag aactgcacgc
    22021 gctgcattca tttcagcttg ccgtagtggg gagtcgggcg cattcatggt atggcgagcg
    22081 atggggacgg ttattttgcg aaactctggc gacgcgtgga gtgacaatta cgagtggact
    22141 ggcgcgtgga atcgatggtg tggcgcataa agctgcctta caggtaaatg gcgtcagcat
    22201 tgctgtattg gggaatggac ttaataccat tcatccccgc cgccatgccc gactggctgc
    22261 cagtctgctt gaacatggcg gagctctcgt ctcggaattt cccctcgatg ttccacccct
    22321 tgcttacaat ttcccacgaa gaaatcgcat tatcagtggt ctaagtaaag gtgtactggt
    22381 ggtggaagcg gctttgcgca gtggttcgct ggtgacagca cgttgtgcgc ttgagcaggg
    22441 gcgtgaagtt tttgccttgc caggtccaat agggaatccg ggaagcgaag ggcctcactg
    22501 gttaataaaa caaggtgcga ttcttgtgac ggaaccggaa gaaattctgg aaaacttgca
    22561 atttggattg cactggttgc cagacgcccc tgaaaattca ttttattcac cagatcagca
    22621 agacgtggca ttgccatttc ctgagctcct ggctaacgta ggagatgagg taacacctgt
    22681 tgacgtcgtc gctgaacgtg ccggccaacc tgtgccagag gtagttactc aactactcga
    22741 actggagtta gcaggatgga tcgcagctgt acccggcggc tatgtccgat tgaggagggc
    22801 atgccatgtt cgacgtacta atgtatttgt ttgaaaccta tattcacaca gaagctgagt
    22861 tgcgtgtgga tcaagacaaa cttgaacagg atcttaccga cgcaggattt gatcgagaag
    22921 atatctacaa tgccctgctt tggctggaaa agcttgcgga ttatcaggaa ggactggcag
    22981 aaccgatgca actggcctct gatccactct ccatgcgtat ttatacccct gaggagtgtg
    23041 aaagactgga tgccagctgt cgtggttttc tgcttttcct tgagcagatt caggtgctca
    23101 accttgaaac tcgtgaaatg gtgatagagc gagtgctcgc gctggataac gcagagttcg
    23161 aactggatga tctgaaatgg gtaatcctga tggtgttgtt caatattccg ggctgcgaaa
    23221 atgcgtacca gcaaatggaa gaattactct ttgaagtgaa tgaaggtatg ctgcattaat
    23281 catttcttta attcagcata agttgttatg gcgaaatcag cactgttcac ggtgcgtaat
    23341 aatgagtcct gcccaaagtg cggggctgaa ctggttattc gatccgggaa acacggtccg
    23401 tttcttggat gctcacagta tccggcgtgt gactacgtcc gtcctctgaa atcttcagcg
    23461 gatggacata ttgtcaaagt tctggagggg caggtttgcc ctgcatgtgg cgcaaatctg
    23521 gtattacgcc agggacgctt tggtatgttt attggttgca gtaactaccc tgaatgcgaa
    23581 cataccgaac ttatcgataa accggacgaa acagcaatta catgccccca atgtcggacg
    23641 ggccatctgg tccagcgccg ctcccgttat ggcaaaacat ttcactcctg tgatcgctac
    23701 ccggagtgtc aatttgccat taacttcaaa ccaatagctg gagaatgccc tgagtgtcat
    23761 tatccgctac tcatcgaaaa gaaaaccgcg cagggtgtaa aacacttttg tgccagtaaa
    23821 caatgtggaa agccggtttc ggcggaataa taacgtgaat aataacctgc aaggagacgc
    23881 tatcgcagct gcgatagatg tcctcaatga agaacgtgtc atcgcctatc caacggaagc
    23941 cgttttcggt gttgggtgcg atcctgatag cgaaacagca gtgatgcgac tgttggagtt
    24001 aaaacagcgt ccggttgata aggggctgat tctaatcgcc gcaaattacg agcagcttaa
    24061 accctatatt gatgacacca tgctgactga tgcgcagcgt gaaaccattt tttcccgctg
    24121 gccaggtcct gtcacctttg tctttcccgc gcctgcgaca acaccgcgct ggttgacggg
    24181 ccgctttgat tcgcttgctg tacgagtcac cgaccatccg ttggtggttg ctttgtgcca
    24241 ggcttatggt aaaccgctgg tttctaccag tgccaacttg agtggattgc caccttgtcg
    24301 aacagtagac gaagttcgcg cacaatttgg cgcggcgttc ccggttgtgc ctggtgaaac
    24361 gggggggcgt ttaaatcctt cagaaatccg cgatgccctg acgggtgaac tgtttcgaca
    24421 ggggtaacat aatggaaacc tatgctgttt ttggtaatcc gatagcccac agcaaatcgc
    24481 cattcattca tcagcaattt gctcaacaac tgaatattga acatccctat gggcgcgtgt
    24541 tggcacccat caatgatttc atcaacacac tgaacgcttt ctttagtgct ggtggtaaag
    24601 gtgcgaatgt gacggtacct tttaaagaag aggcttttgc cagagcggat gagcttactg
    24661 aacgggcagc gttggctggt gctgttaata ccctcaagcg gttagaagat ggacgcttgc
    24721 agggtgacaa taccgatggt ataggtttgt taagcgatct ggaacgtctg tcttttatcc
    24781 gtccgggttt acgtattctg cttatcggcg ctggtggagc atctcgcggc gtactactac
    24841 cactcctttc cctggactgt gcggtgacaa tcactaatcg gacggtatcc cgcgcggaag
    24901 agttggctaa attgtttgcg cacactggca gtattcaggc gttgggtatg gacgaactgg
    24961 aaggtcatga gtttgatctc attattaatg caacatccag tggcatcagt ggtgatattc
    25021 cggcgatccc gtcatctctc attcatccag gcatttattg ctatgacatg ttctatcaga
    25081 aaggaaaaac tccttttctg gcatggtgtg agcagcgagg ctcaaagcgt aatgctgatg
    25141 gtttaggaat gctggtggca caggcggctc atgcctttct tctctggcac ggtgttctgc
    25201 ctgacgtaga accagttata aagcaattgc aggaggaatt gtccgcgtga atcaggccat
    25261 ccagtttccg gacagagaag agtgggacga gaataaaaaa tgtgtatgtt ttcccgctct
    25321 cgtgaatggc atgcaactga catgtgcgat ctctggcgag agtctggcgt atcgttttac
    25381 tggagatacg ccagaacagt ggttagcgag ttttcgtcag catcgctggg atctggaaga
    25441 agaagcggaa aacttaattc aggaacaaag tgaagatgat caaggctggg tctggttacc
    25501 ctgatccaga tattcgtcct tccatttcac gtaattattc gcggaatagc gtaacccagc
    25561 cttctcttca tcacttaacg ggcggatctg tttgacgggg ctaccgagat acagatatcc
    25621 gctctccagc cgtttatttt gtgggaccag actacccgca ccaatcatca catcatcttc
    25681 tactattgcg ccatcaagta aaattgagcc catcccaacc aaaactcgat tgccaatggt
    25741 gcagccgtgg agcatcacct tgtgaccaac agtgacatct tcgccaatgg ttaatgggtt
    25801 gccatctggg ttgtacgagg atttatgagt gacatgcaac atactgccat cctggatatt
    25861 ggtgcgtgct ccgatctgta cataatgtac atctccacga atcacaacga gcggccagat
    25921 ccccacatca tcagccagac gaacgtcacc aatcacgaca ctgctatcgt cgatcattac
    25981 gcgctgaccg atttgtggaa aaagatcgcg gtatgggcgt aaaacatcag acatacttac
    26041 ctcagcaata aatgatttac taatgacttt gggggcatta ttggccttgt gcaagtcttt
    26101 tagtatgcaa aaaagcaccg ttttgtgtgc gattgcagca aaaagggtga aaaaacaaca
    26161 aacagaaaaa aagatcaaaa aaatacttgt gcaaaaaatt gggatcccta taatgcgcct
    26221 ccgttgagac gacaacgtga aacacttcac aggatggtcg gaacaacgaa gagaaaaaat
    26281 cctgaaattc agggttgact ctgaaagagg aaagcgtaat atacgccacc tcgcgacagt
    26341 gcgctaaag
//
LOCUS       DAZKIV010000054        26218 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZKIV010000054 DAZKIV010000000
VERSION     DAZKIV010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.054"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1222)
                     /gene="paaK"
                     /locus_tag="ACHOT6_002218"
     CDS             complement(<1..1222)
                     /gene="paaK"
                     /locus_tag="ACHOT6_002218"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFP9947434.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGIS"
     gene            complement(1249..2454)
                     /gene="paaJ"
                     /locus_tag="ACHOT6_002219"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(1249..2454)
                     /gene="paaJ"
                     /locus_tag="ACHOT6_002219"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFP9947435.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRSAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(2454..2876)
                     /gene="paaI"
                     /locus_tag="ACHOT6_002220"
     CDS             complement(2454..2876)
                     /gene="paaI"
                     /locus_tag="ACHOT6_002220"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFP9947436.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(2866..4293)
                     /gene="paaH"
                     /locus_tag="ACHOT6_002221"
     CDS             complement(2866..4293)
                     /gene="paaH"
                     /locus_tag="ACHOT6_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFP9947437.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVKRGKLTAETCERTLKRLIPVTDIHALAAANLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAVPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGTQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(4295..5083)
                     /gene="paaG"
                     /locus_tag="ACHOT6_002222"
     CDS             complement(4295..5083)
                     /gene="paaG"
                     /locus_tag="ACHOT6_002222"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFP9947438.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLIRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLAWHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(5083..5850)
                     /gene="paaF"
                     /locus_tag="ACHOT6_002223"
     CDS             complement(5083..5850)
                     /gene="paaF"
                     /locus_tag="ACHOT6_002223"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFP9947439.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(5847..6917)
                     /gene="paaE"
                     /locus_tag="ACHOT6_002224"
     CDS             complement(5847..6917)
                     /gene="paaE"
                     /locus_tag="ACHOT6_002224"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFP9947440.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASLDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYARDHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDDAETALKALGMPEKAIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(6925..7422)
                     /gene="paaD"
                     /locus_tag="ACHOT6_002225"
     CDS             complement(6925..7422)
                     /gene="paaD"
                     /locus_tag="ACHOT6_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFP9947441.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMSTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVCCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(7437..8183)
                     /gene="paaC"
                     /locus_tag="ACHOT6_002226"
     CDS             complement(7437..8183)
                     /gene="paaC"
                     /locus_tag="ACHOT6_002226"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFP9947442.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(8192..8479)
                     /gene="paaB"
                     /locus_tag="ACHOT6_002227"
     CDS             complement(8192..8479)
                     /gene="paaB"
                     /locus_tag="ACHOT6_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFP9947443.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(8491..9420)
                     /gene="paaA"
                     /locus_tag="ACHOT6_002228"
     CDS             complement(8491..9420)
                     /gene="paaA"
                     /locus_tag="ACHOT6_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFP9947444.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGVEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMAVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            9705..11750
                     /gene="paaZ"
                     /locus_tag="ACHOT6_002229"
     CDS             9705..11750
                     /gene="paaZ"
                     /locus_tag="ACHOT6_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFP9947445.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLLHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLENLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            11998..14271
                     /gene="tynA"
                     /locus_tag="ACHOT6_002230"
     CDS             11998..14271
                     /gene="tynA"
                     /locus_tag="ACHOT6_002230"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFP9947446.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(14328..15827)
                     /gene="feaB"
                     /locus_tag="ACHOT6_002231"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(14328..15827)
                     /gene="feaB"
                     /locus_tag="ACHOT6_002231"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFP9947447.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            16063..16968
                     /gene="feaR"
                     /locus_tag="ACHOT6_002232"
     CDS             16063..16968
                     /gene="feaR"
                     /locus_tag="ACHOT6_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFP9947448.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLDTHFAKGL
                     KLSTVTTNGVNLSRTWQEIKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(17140..17469)
                     /locus_tag="ACHOT6_002233"
     CDS             complement(17140..17469)
                     /locus_tag="ACHOT6_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFP9947449.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(17474..17659)
                     /locus_tag="ACHOT6_002234"
     CDS             complement(17474..17659)
                     /locus_tag="ACHOT6_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFP9947450.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(17656..20295)
                     /locus_tag="ACHOT6_002235"
     CDS             complement(17656..20295)
                     /locus_tag="ACHOT6_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFP9947451.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKNLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            20503..21492
                     /gene="ldhA"
                     /locus_tag="ACHOT6_002236"
     CDS             20503..21492
                     /gene="ldhA"
                     /locus_tag="ACHOT6_002236"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9947452.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKTQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKSETCPNELV"
     gene            21603..22025
                     /gene="hslJ"
                     /locus_tag="ACHOT6_002237"
     CDS             21603..22025
                     /gene="hslJ"
                     /locus_tag="ACHOT6_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFP9947453.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(22022..22288)
                     /locus_tag="ACHOT6_002238"
     CDS             complement(22022..22288)
                     /locus_tag="ACHOT6_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFP9947454.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            22562..26086
                     /gene="nifJ"
                     /locus_tag="ACHOT6_002239"
     CDS             22562..26086
                     /gene="nifJ"
                     /locus_tag="ACHOT6_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFP9947455.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         6351 a         7050 c         6491 g         6326 t
ORIGIN      
        1 tagagatccc caccatcgac ttaatccgat ggcgcagctg gtggcatacc tggcaacgct
       61 gctcatgcgt caatgtcagg ctactttctt tcaactccac tttcacagaa agtgaatcaa
      121 gatgcccccg gcggttcacc tccagttggt aatgcggcga taaatgttcg aatttgacaa
      181 tctcttcttc cagttgtgac ggaaagacat ttacaccgcg aataatcaac atgtcatcgc
      241 ttcgtccgct gatgcgatcc atgcggcgca tagtccgcgc cgttcctggt aacagtcggg
      301 ttaaatcacg cgtgcggtaa cgaatgaccg gcaatgcttc tttggtcagc gtggtgaata
      361 acagttcgcc atgttcacca tcggcaagcg gtgtgccgtc atgaggatta acaatttcag
      421 gatagaaatg atcttcccaa atagttggac cgtcggtagt ttccagacac tccatcgcca
      481 cccccggccc catcacttct gacaggccat aaatatccag tgcggtgatc cccagacgcc
      541 gttcaatctc tttacgcatg gcctgtgtcc acggctcagc accaaacacg ccaacccgca
      601 gcgagcaacc gctggcatca ccgcccaact gccgctccag ctcttcaatc aggttaaggc
      661 aataagatgg cgtaaccatg atcatatctg gttgaaaatc gcggatcagt tgcgcttgtt
      721 tttccgtctg cccgccagac atcgggatca ccgtagcgcc cagacgttcg gcaccatagt
      781 gcgcgcccag cccaccagta aacaggccgt aaccataggc aacatgaatt ttgtctttgg
      841 gcgagccccc tgccgcacgc aaagaacgcg ctacaatatt ggcccagtta tcaatatcgt
      901 tttgcgtata accgacaact gtcggtttac ctgtggttcc cgaagaagca tgaatgcgca
      961 ccacttgttc catcggcacg gcaaaggtgt caaagggata gttgtcgcgc agatcctgtt
     1021 tggtagtaca gggaaattta cgcaggtctg aaagttccct gaaatcatca ggatgtacgc
     1081 ctgctgcgtc gaatttgcgc cgatacatcg ggacattttc atacgcgtgt ttgagcgtcc
     1141 atttcagacg ctgtgtttgc aacgcctgta actcatcaac agacgcggtt tcaatcgggt
     1201 caagctttgt attggttatc attgtagggt actcgcaggt tgatatgctc aaacacgctc
     1261 cagaatcatg gcgatgccct gaccgacacc gatgcacatg gtgcataatg cgtaacgacc
     1321 gttacgccga tgcagctcat ggctggcagc cagtgccagg cgggcaccac tcattcccaa
     1381 cggatggcct aaggcgatag cgcctccgtt ggggttaaca tgtggggcat catcaggcag
     1441 ccccaattcg cgtagtacac ccaacgcctg ggccgcgaac gcttcgttca attcaatcac
     1501 gtccatatcg tgaatactca gccccgcgcg ttccagcacc cggcgagttg caggcaccgg
     1561 accaagcccc atcaggcgcg gttccacccc ggcggttgcc atggctacga tacgcgctcg
     1621 cggtgtcagt ccttgcgctg ctgccatctg ttcactggca ataatcaacg cagcggctcc
     1681 gtcattcacc ccggaggcat tgcctgcggt gatcactcca ttggcacgaa atggtgcttt
     1741 tagtccacgt aactgttcca gcgtcgtttc cgggcgcaaa tgctcgtcat gttgtatttc
     1801 tgttacaaca cctttcttat ttttcaacac aaccggaaca atctcttcag ctaaaatgcc
     1861 tgaagattgc gcttttgccg aacgttgctg actgcgtagc gcaaaactat cttgatcttc
     1921 tcgcgagatt tttaacagtt cagctacatt ctctgccgtt tccggcatgc tgtcagttcc
     1981 aaattgttga gccatgagcg ggttcacaaa tcgccagcca atagtggtat caaacatctc
     2041 agcctgacga gaaaatgcac tggttgcctt gcccataaca aacggtgccc gagacattga
     2101 ctccacgcca ccggcgatca gcaaatcgcc atcgcccgct ttaatcgccc gtgcggcgaa
     2161 ccccagtgcg tccagcccgg aaccacacaa gcggttaatg gttgtgccgg aaacactctg
     2221 cggcagcccc gccagtaaag tcgccatccg ggctacgtta cggttatctt ctcccgcctg
     2281 attagcacag ccgaggatca catcatcgat acactccgca tcgagacgcg ggtttcgcac
     2341 cagcagctcc cgcaaaggga tagcagccag atcgtcagcc cgaacacctg ataatgcccc
     2401 accatagcgg ccaattggcg tacgaattcc gtcacagata aaggcttcac gcatcaggct
     2461 tctcctgtaa tggtgccgcc gatgcggtga gatttaccgc gaaacagcgc aaccgttttt
     2521 tgttgttggt taacaatttc gatgtcgtaa acaccggttt gcttgccctg atgacgtacc
     2581 tgcgcagtag cagttaaggt gtctccggca aagcctggac gcaaaaaatc aatcgtgcag
     2641 gcagaagcga cggctgccag cccctggcta ttgcaggcgt aggcaaaggc agtatcagcc
     2701 agtgaaaata gctgcccgcc gtggcaactt tgatgaccat taagcatttg tgcagtgacg
     2761 gtcatggtca ctacagcaaa gccttcatcc attgagataa tgtcgatgcc aagcgcttta
     2821 gcgcaggcat cgttctcata cattgcatgg gcattttgcc aggccttatg actcataacc
     2881 gctctccaga agcgctcgtt ggcgcagcaa tgaacatggg cgatagcgtt cttcgccata
     2941 gtgatgttgt agattttcaa ggagccttaa tattcgctgc cagccaagtt gcgttcccca
     3001 ggcaagcggg ccatatggat aattcacccc aagacgcatg gcggtatcga tatcctgttc
     3061 agaggccacg cctttttgca gcgcatcaag ggcttcattg atgatcattg ctaccgttcg
     3121 ccaaatcagc attcctgggt aatctgcaat ttgcagcact gttttgccct gctgttgcag
     3181 gtaataaatg gccttgcggg tcgctgagtc cgggttcacc gctgccgcag caatggtcac
     3241 caccttgccc gccattttgt cgaccactac caccgggcgt gctagtcgta ttgccagcgc
     3301 ctgtgccgtc tcgccttgtg tctcaatcaa taaaacatcg tcaatttccg tgacaccgtc
     3361 acttttcttt tctactttca ttgggctaaa actgtcgctt accgcttcca ggccaacaac
     3421 tgcctcacgt tccgcgcgcc agtcgtacac gcccagccca cttttcttgc ccaaccgtcc
     3481 accaatcacc agttcctgtt gcaccagcga aggtaaaaaa cgacgctcct gccagaaagc
     3541 gttaaacacc gaacaggtga cagcaaaatt gacgtcctga ccaatcagat cggttaattc
     3601 cagcggcccc atcgggaaac cagcgccatc gcgaagtgca gcgtcaatca cttctggtac
     3661 agcaacctgc tcttccagtg cccgccaggc ctcggaataa taaggacgcg caacacggtt
     3721 aacgataaat ccaggagtcg aatgacagcg cacaggctgc ttaccccaac tcaacgttag
     3781 ttcacacaac tgctcaacaa cttccgccgc cgttgccagc ccactgacca cctccaccaa
     3841 cttcattacc ggtgccgggt taaaaaaatg cagccccgca acacgttcag gatttttcac
     3901 ctccgcagca atcgcggtaa tagagattga cgatgtgtta gtggtcaata gcgtttgtgg
     3961 cgggcaaact tccgccagct gtgcaaagag cgcttttttg acttccagac gttcagacgc
     4021 cgcttcaata accagattcg ctgctgccag cgcatgaata tcggtcaccg ggatcaggcg
     4081 tttcaatgtg cgttcacaag tttcagcggt cagttttccc cgcttaacgc gtgaatttag
     4141 ccgcgcgtgt atcccgtcga ttgcgcgggt cagcgcttca gcagaaatgt catacagtaa
     4201 aacctgatgt ccatgactgg cggcaacttc agcaatgcct gcccccatgg tgccgctccc
     4261 aatcactgcc acagtttgca catttatcat catgctattt ccccgtgaac tgcggtgagc
     4321 gtttagccag gaacgcactg acaccttcac gataatctgc gctccgtccg gcaagtcgct
     4381 gatagtcgcg ttccagatcc agttgcgtat cgagcgtatt ggtttcagcg ctatttatcg
     4441 cttgcttgat aagtccaaga ccaaatgtcg gttgtgtcgc cagatgccat gccagctgtt
     4501 gcgcggtatc tgccagcgtt tcatcatcaa caacctgcca gatcatcccc cattcgtgcg
     4561 cctgttcagc actcagttga ttccccagca gtgccagccc catggcgcgc gctcgtccgg
     4621 caacgcgtgg cagtaaccag gttccaccgc aatcgggtac taagcctaac ttactaaacg
     4681 ccatgacgaa ttttgctgaa cgggcagcaa taacgatgtc gcatcccagt gccagtgttg
     4741 cgccagcccc tgccgccacg ccattgactg cacagatcac cggttttggc agttttgcca
     4801 ggcgacgtat cagtgggtta tagaaacgtt caactgacat ccctaaatcc ggtgcggggc
     4861 ctgtgggatc aacgttacga tcgttaagat cctgaccagc acaaaatccg cgcccggcac
     4921 ctgtaagtaa caggcaacgg atagtgtcgt cgcgctcgac ctgtttcaga cactctgcca
     4981 gttgtgcgtg catctcatca ttaaaactgt tcaggcgttc cgggcggttg agcgttagtg
     5041 tcatcacgcc cttttctaca tgactgagga tgaattccat cattagcgtc ctttaaagtc
     5101 gggcgtgcgt ttttgtaaga aagcggagat gccttcatga cgatcttctg ttgccgccag
     5161 caaggtgaat aactgtcgct cctgggcaag tccggcttgc aaagccactt cctgcgactg
     5221 gcgcagcgct tgctttgccg cttgtaaggc cagcggcgag tgacgtgcca ttttcgacgc
     5281 cagctgtaag gcgtattcga gggttaaatc gctggggaag acgtcgctaa ccagcccggc
     5341 ctgctgtgct tgctgagcgg tgatactttc tccgctcagc accattttgc tggctaacga
     5401 tttaccgaca ctacggatta aacgttgcgt tcctcctgcg cctggcatga tgccgagagt
     5461 gatttccggc aaaccaaaac gcgcgttctc tccggcaacc accacatcgc acaacaatgc
     5521 cagttcgcaa cccgcaccaa gcgcgtaacc gttgacggct gcgatgagag gtttgttgaa
     5581 ggcctgcaat cgcgcccata gctgcgggcg tgtatcgttt aaggtggccg cgagatcttt
     5641 ttctgccatt tcgttgagat cggccccagc ggcaaaaaag cgtgcattac cggtaatcac
     5701 acagaccgaa atgctgctat cggtagccgc agcttccagt tcatttacca gttgcgtcag
     5761 aagggcatta tttagcgcat tacgtgcggc gggacggtta agggtcagca acaatacttg
     5821 ttgctgacgg ctgacgatca gttcgctcat gccatcccct tcgcgtcaaa gtcaaccacc
     5881 acatcgctgg tcagcggcag tgcctggcaa ctcaacacat agcctgcggc cagttcatcc
     5941 ggttccagac tgtaattggt ttccatcgcc actttgccac gcagcacttt gcatttgcag
     6001 gtcgcacaga cgccgccttt gcaggcatag ggcagatcag ccccctggcg caatgccgca
     6061 tcgagaatgc tttcatcgtc ggcattaagc acgatttccc gatcccgccc atcctgacgt
     6121 acagtcactt tttgtccgtc actttgcacg ttaacgctac gtttgacgcg cgtgccaggc
     6181 gtattaaacc gctccagatg aatcgctttt tctggcattc ccagtgcttt taaggcggtt
     6241 tccgcgtcat ccatcatcgc cgccggccca caaataaatg cctcatcata aagacgaaaa
     6301 ttaatgagcg aggccccaag tgactgtaat ttttcaccgt caatacgccc gtgaagcaga
     6361 tcgctgtcga gggtttcctg actgaaaatg cacaacaact gtaaacgctg aggatatttg
     6421 tctttcaggt ctgccagtgc ctggcgaaac atcatgctct ggctggtacg gttaccgtag
     6481 attagggtga actgactttc aggctcggtt tgtaaagtgg tggcgataat cgccagcatt
     6541 ggcgtaatac ctgatcctgc tgcaatagcc agatagcgcc cctggcgttc ggcctgcggc
     6601 tgatagccga aatggccctg cggcaccatg acttccagcg tcatcccctg acggatgtga
     6661 tcgcgggcat agcgggagaa acgtccgcct tcaatggctt ttaccgccac actaatttcg
     6721 ccaggcaggt agctgcggca aatggagtaa caacggcgta attcttcacc atcaaggctg
     6781 gcttttaagg tcaagtgttg accggggcga aagcgatacg cctcctgcaa aggctggggc
     6841 accgcaaagg taatggtcac cgcatcacgg gtttccggct ccacttttgc caccgttaag
     6901 gaatgaaacg ttgtcatggc atcctcaaat acatttgaaa taatcgaaag gttcgcggca
     6961 actatcgcag cgatacaatg ctttgcaggc cgtggaacca aattcactga taagtgtggt
     7021 atggacgctg gcacagcgcg ggcaacatac ttctggcggc aaatgggcat ggcaactgtg
     7081 tccggcgggc gggctaatgc catactgtcg cagacgctca cgggcatcgg gggtcatcca
     7141 gtcggtggtc catgccgggt caagttgcag cacaacctga acgggggtaa acccgtgggt
     7201 tgacattgcc tcacgtatcg cgccaatcaa atgttccgtt gccggacaac cggaatatgt
     7261 cggcgtaaag ccgatcaccc atccttctcc catctgtgtc acattacgca ccatgcctaa
     7321 atcagtaatg gtcagcaccg ggatctccgg gtccgggatc tggctgagta gcgcccatat
     7381 ctcatgaacc tgtggcggtg caatggtggc cagacgttgc atacccatct cctctgttac
     7441 cattgctgac cgggcaagac acgctggaga tactgcattt ctgccagcat gggtccaagg
     7501 tgttcggtat gcagtccttt tttaccgcca gtgcgatacg cctgctcttg cggtacgttc
     7561 aatgtggctt cgttgatccc ggcaaaaact tcggcttccc acgctgcgcg taaagtgcgt
     7621 ggatcaaccg caataccctc ttcactcagt gcaatatcaa tctcgtcggc atcgaacagt
     7681 tcggcggtaa aacgccacaa cttgttgatg gcctgctgca tcttttgccc tgatacgtca
     7741 gtaccattgc ccagtcgctc cagccagcca cgactaaaac gcaggtgata acgcgcttct
     7801 ttaattgctt tggcagaaat cgccgccagt tgcggatcac ggctctccat cagacgggta
     7861 aagagcgcca catgccatgc atcgatgaaa tactggcgtg caatggtgtc ggcaaaattg
     7921 ccgtttggct gttcaaccaa caataagttg ctgaactggc gctcgtctcg ggtgaaggcc
     7981 agggtatctt catcgccttc tcccgctaat tcagcggcat acgataagaa gttgcgtgcc
     8041 tggcctaata aatcgagtcc aatatttgcc agtgcgagat cgatttccag ttccggtgca
     8101 tgaccgcacc attcacccaa ccgctgggag agcaccaggc agttatcgcc caggcgcaag
     8161 gtgtaagccg ttaactgatt catttccgac ctcacatgtg ctcaatgcca tcagggatgg
     8221 tgtaaaacgt tggatggcga tagaccttgc tttcagccgg atcaaaaaat tcaccgcgtt
     8281 cttccggttg cgaggcaaca atttcactcg ccttcaccac ccaaattgaa catccttcgc
     8341 tacgacgggt gtaagcatca cgggcatttt ccagtgccat ccgctcatcg gcagcatgta
     8401 aactgccaac atggcggtgt gataagccct gtttgccacg cacgaaaact tcgtataacg
     8461 gccagtaaac attactcatt ttgaatctcc ttatgcgacc ttacgggcaa gttgtttttg
     8521 tgcatgggcc agcgctgctt cccgtaccca ggtaccttct tcccaggctt tacgtttggc
     8581 gtcgagcctc tcctgattac aaattccgcg accgttaatt acttcgttaa actcctgcca
     8641 gtcgatctca ccaaagcggt agtgaccgct ttcagtgtca aaatgcagat ccgggtcagg
     8701 aacggccata ccgagcattt caacctgtgg aacggtgttg tccacgaaac gctggcggag
     8761 ttcgtcgttg gtgaaacgtt tgattttcca ggcgagactt ctggcgctgt ttggcgagtt
     8821 atcatcgttt ggcccgaaca tcattaaggc tggccaccag aaacggttaa tggcgtcttg
     8881 caacatctgc ttttgagctt cgctgccctg cgccagtgcc atgcaggctt caaccccctg
     8941 acgctggtga aaactctctt ctttgcagat tttcaccatt gctctggcat acgggccata
     9001 agaagtgcgg cataacgcca cctgattgac gatcgctgcg ccatccacca gccagccgat
     9061 aacgccgata tcagcccaac tcaatgtcgg ataattaaag atggaggaat atttcatccg
     9121 tccgtcgagc attttttggt agatgtcctc tctggcgcag cccagtgttt cggcggcgct
     9181 atagagatac agaccatgac cggcttcatc ctgcactttg gccaacagaa tggctttacg
     9241 ccgcaaggtt ggtgcgcgag tgatccagtt accttcaggc aacataccaa caatctcgga
     9301 gtgcgcatgc tgcccaatct ggcggatcag tgtctttcgg taagcatcgg gcatccagtc
     9361 ctgtggctcg atagccgttt cctgggctat ccgttgctca aagcgttctt cttgggtcac
     9421 gtcatcacct ttacgattcc agttttcgtg aattaattaa aaacacgaat cacattgttt
     9481 tatgaaagtt acacaatggt taactaaaat cacaaatttt gtatgcttga actgtgacgg
     9541 atttcgcacc ctatttttat aaaccattat ttatcagtgc attaatgttt tccgcagttg
     9601 ctatacagat cgcatagtta acatttcgtt aaaagacccc ttgcttttta tgattcgcga
     9661 tttaactatt agcaacagaa atgtgaaaca tctggagagt agcgatgcag cagttagcca
     9721 gtttcttatc cggtacctgg cagtctggcc ggggccgtag ccgtttgatt caccacgcta
     9781 ttagcggcga ggcgttatgg gaagtgacca gtgaaggtct tgatatggcg gctgcccgcc
     9841 agtttgccat tgaaaaaggt gcccccgccc tccgcgcgat gacctttatc gaacgtgcgg
     9901 cgatgcttaa agcggtcgct aaacatctgc tgagtgaaaa agagcgcttc tatgctcttt
     9961 ctgcgcaaac aggcgcaacg cgggcagaca gttgggttga tattgaaggt ggcattggga
    10021 cgttatttac ttacgccagc ctcggtagcc gggagctgcc tgacgatacg ctgtggccgg
    10081 aagatgaatt gatcccctta tcgaaagaag gtggatttgc cgcgcgccat gtactgacct
    10141 caaagtcagg cgtggcagtg catattaacg cctttaactt cccctgctgg ggaatgctgg
    10201 aaaagctggc accaacgtgg ctgggcggaa tgccagccat catcaaacca gctaccgcga
    10261 cggcccaact gactcaggcg atggtgaaat caattgtcga tagtggtctt gttcccgaag
    10321 gcgcaattag tctgatctgc ggtagtgctg gcgacttgtt ggatcatctg gacagccagg
    10381 atgtggtgac tttcacggga tcagcggcga ccggacagat gctgcgagtt cagccaaata
    10441 tcgtcgccaa atctatcccc ttcactatgg aagctgattc cctgaactgc tgcgtactgg
    10501 gcgaagatgt caccccggat caaccggagt ttgcgctgtt tattcgtgaa gttgtgcgtg
    10561 agatgaccac aaaagccggg caaaaatgta cggcaatccg gcggattatt gtgccgcagg
    10621 cattggttaa tgctgtcagt gatgctctgg ttgcgcgatt acagaaagtc gtggtcggtg
    10681 atcctgctca ggaaggcgtg aaaatgggcg cactggtaaa tgctgagcag cgtgccgatg
    10741 tgcaggaaaa agtgaacata ttgctggctg caggatgcga gattcgcctc ggtggtcagg
    10801 cggatttatc tgctgcgggt gccttcttcc cgccaacctt attgtactgt ccgcagccgg
    10861 atgaaacacc ggcggtacat gcaacagaag cctttggccc tgtcgcaacg ctaatgccag
    10921 cacaaaacca gcaacatgct ctgcaactgg cttgtgcagg cggcggtagc cttgcgggaa
    10981 cgctggtgac ggctgatccg caaattgcgc gtcagtttat tgccgacgcg gcacgtacgc
    11041 atgggcgaat tcagatcctc aatgaagaat cggcaaaaga atccaccggg catggctccc
    11101 cactgccaca actgttacat ggtgggcctg gtcgcgcagg aggcggtgaa gaattaggtg
    11161 gtttacgagc ggtgaaacat tacatgcagc gaaccgctgt tcagggtagt ccgacgatgc
    11221 ttgccgctat cagtaaacag tgggtgcgcg gtgcgaaagt cgaagaagat cgtattcatc
    11281 cgttccgcaa atattttgag gagctgcaac caggcgacag cctgttgact ccccgccgca
    11341 caatgacaga ggccgatatt gttaactttg cttgcctcag cggcgatcat ttctatgcac
    11401 atatggataa aattgctgct gccgaatcta tttttggtga gcgtgtggta catggatatt
    11461 ttgtgctttc tgcggctgcg ggtctgtttg tcgatgccgg tgtcggtccg gtcatagcta
    11521 actacgggct ggaaaacttg cgttttatcg aacccgtaaa accaggcgat accatccagg
    11581 tgcgtctcac ctgtaagcgc aagacgctga aaaaacagcg tagcgcagaa gaaaaaccaa
    11641 caggtgtggt ggaatgggcg gtagaggtat tcaatcagca tcaaaccccg gtggcgctgt
    11701 attcaattct gacgctggtg gccaggcagc acggtgattt tgtcgattaa tcggtgaatg
    11761 aagggcaacg gcgaatagtt gcccttttat ttcactaaat tttgtgacgt tgtcacatta
    11821 tgcatgatgt gtacatctat tttcagggca tccactgtat gaaaagctgg cacacctgcc
    11881 aaacaacctg gcaggtgcag gcaatcccct ttgcatcagt actgataatg tgaacctgac
    11941 taaaccgccc acagagcgcg gttgctaaca agaacacaac atctgacgag gttaataatg
    12001 ggaagcccct ctctgtattc tgcccgtaaa acaaccctgg cgttggcagt cgccttaagt
    12061 ttcgcctggc aagcgccggt atttgcccac ggtggtgaag cgcatatggt gccaatggat
    12121 aaaacgctta aagaatttgg tgccgatgtg cagtgggacg actacgccca gctctttacc
    12181 ctgattaaag atggcgcgta cgtgaaagtg aagcctggtg cgcaaacagc aattgttaat
    12241 ggtcagcctc tggcactgca agtaccggta gtgatgaaag acaataaagc ctgggtttct
    12301 gacaccttta ttaacgatgt tttccagtcc gggctggatc aaacctttca ggtagaaaag
    12361 cgccctcacc cacttaatgc gctaactgcg gacgaaatta aacaggccgt tgaaattgtt
    12421 aaagcttccg cggacttcaa acccaatacc cgttttactg agatctccct gctaccgcca
    12481 gataaagaag ctgtctgggc gtttgcgctg gaaaacaaac cggttgacca gccgcgcaaa
    12541 gccgacgtca ttatgctcga cggcaaacat atcatcgaag cggtggtgga tctgcaaaac
    12601 aacaaactgc tctcctggca acccattaaa gacgcccacg gtatggtgtt gctggatgat
    12661 ttcgccagtg tgcagaacat tattaacaac agtgaagaat ttgccgctgc cgtgaagaaa
    12721 cgcggtatta ctgatgcgaa aaaagtgatt accacgccgc tgaccgtagg ttatttcgat
    12781 ggtaaagatg gcctgaaaca agatgcccgg ttgctcaaag tcatcagcta tcttgatgtc
    12841 ggtgatggca actactgggc acatcccatc gaaaacctgg tggcggtcgt tgatttagaa
    12901 cagaaaaaaa tcgttaagat tgaagaaggt ccggtagttc cggtgccaat gaccgcacgc
    12961 ccatttgatg gccgtgaccg cgttgctccg gcagttaagc ctatgcaaat cattgagcct
    13021 gaaggtaaaa attacaccat tactggcgat atgattcact ggcggaactg ggattttcac
    13081 ctcagcatga actctcgcgt cgggccgatg atctccaccg tgacttataa cgacaatggc
    13141 accaaacgca aagtcatgta cgaaggttct ctcggcggca tgattgtgcc ttacggtgat
    13201 cctgatattg gctggtactt taaagcgtat ctggactctg gtgactacgg tatgggcacg
    13261 ctaacctcac caattgctcg tggtaaagat gccccgtcta acgcagtgct ccttaatgaa
    13321 accatcgccg actacactgg cgtgccgatg gagatccctc gcgctatcgc ggtatttgaa
    13381 cgttatgccg ggccggagta taagcatcag gaaatgggcc agcccaacgt cagtaccgaa
    13441 cgccgggagt tagtggtgcg ctggatcagt acagtgggta actatgacta catttttgac
    13501 tggatcttcc atgaaaacgg cactattggc attgatgccg gtgctacggg catcgaagcg
    13561 gtgaaaggtg ttaaagcgaa aaccatgcac gatgagacgg cgaaagatga cacgcgctac
    13621 ggcacgctta tcgatcacaa tatcgtgggt actacacacc aacatattta taatttccgc
    13681 ctcgatctgg atgtagatgg cgagaataac agcctggtgg cgatggaccc agtggtaaaa
    13741 ccgaatactg ccggtggccc acgcaccagt accatgcaag ttaatcagta caacatcggc
    13801 aatgaacagg atgccgcgca gaagtttgat ccgggcacga ttcgtctgtt gagtaacccg
    13861 aacaaagaga accgcatggg caatccagtt tcctatcaaa ttattcctta tgcaggtggt
    13921 actcacccgg tagcaaaagg tgcccagttc gcgccggacg agtggatcta tcatcgttta
    13981 agctttatgg acaagcagct ttgggtaacg cgttatcatc ctggcgagcg tttcccggaa
    14041 ggcaaatatc cgaaccgttc tactcatgac accggtcttg gacaatacag taaagataac
    14101 gagtcgctgg acaacaccga cgccgttgtc tggatgacca ccggcaccac acatgtggcc
    14161 cgcgccgaag agtggccgat tatgccgacc gaatgggtac atactctgct taaaccatgg
    14221 aacttctttg acgaaacgcc aacgctaggg gcgctgaaga aagataagtg attgtttcag
    14281 aaaaaaaacg gcaccaggtg ccgtttttta cttatgagcg aaccagatta ataccgtaca
    14341 cacaccgact tagtttcaca ccaaccgtcc agccagtcgg ggccaaaatc acggcccgtt
    14401 cctgactgct tcatcccacc aaacggtaag ttagcgtcaa ttaaggtatg gctgtttacc
    14461 cacaccgtcc ctgcctgtaa gcgatcgcta tattccagag cctgggagag attttgcgtc
    14521 cagacactgg cagttaagcc atattccgtg tcgtttgcca gttgtaacgc ctcttctcca
    14581 tccgctactc gcaccaggtt taccaccgga ccaaacacct cttcacgagt taagcgcaat
    14641 ttagcatcgg gatttaccac cagcgttggc gcaacataat acccctctcc ggctggtcca
    14701 ttcgacccgc gaatcagctc tgcttgctgt gcctgcgcat cgtcgaggaa tgaacacact
    14761 ttgtcgcagt gcgcacgaga aaccaaaggg ttaatctgtg caacaggtga catccccggt
    14821 cccacttgca acgattttac cgcctgctca aatccactaa ccagcgtgtc aaacaacggc
    14881 gcttcaatat aaattcgcga actggcggcg catacttgcc cttgattcag gaagcttccg
    14941 gtcatcaagc cttcaataac ccattgcgga tcagcatctt ttaatacaat tgccgggttt
    15001 ttaccgccca gttccagcgt tacacgcgtt aagtgatcag cagcagttct ggcaatacct
    15061 tttcccgtcg cggttgaacc ggtaaaactg attttcgcaa catgaggatg tgacgtcagg
    15121 gccgcgccgc atacagcacc tgacccggtg accacattaa aaacgccatc agggatacca
    15181 gcctcgctgg ccagttccgc cacgcgcaac atcgtcagtg gcgtggtttc cgaaggctta
    15241 atcacgattg aacagcctgc tgccagtgct ggcatcacct tccacatgcc aatcatcaac
    15301 ggaaagttcc atggcacaat tcccgctacc acgccaaccg gctctttacg cgtccaggcc
    15361 tgataacgcg ccccctgggg taagggaatc gacaagtcca gcgttttacc cgcgattttg
    15421 gtcgttaacc cggcggtata acgcatccag ttcagcgtac agcccacttc aaaagcacgg
    15481 gaaatggcaa ttgacttgcc ttgctccagg gtttccagtt gcgccagctc ctcactgtgc
    15541 tgctccacca gatcagcaaa acgtagcaga atacgttcac gctctgcggg taatcgcccg
    15601 gcccagcgac gcgagacaaa ggcccgccat gcagacatga ctgcgttatc tacatccgct
    15661 tcgttggcat cagcagttga tgcaatttct tgcccggttg ccggatcaaa gatcgccaac
    15721 cgtttttcac tttgtgcggg gccaggacga ccatcaatat aaagaccgtg ttgacgatcg
    15781 agaaactgtt ggacctggct taatactgct acatgcggct ctgtcatcac ttttccttat
    15841 tatttaccca gtgtgatttc agtgtacgca gcgacaagat aagtcacttt tttcgctgtg
    15901 acattcgctt tgtcagctgt gacaagctcc gcaaatcgtg acaataaccg cttatgtctc
    15961 tatgcctgac aagacttatt tcctatagtc aggctcatta gcttcgatta tcgcaacaca
    16021 aatgcaacaa taaaaacaca tttcacagag cgaaaacgtg ccatgaaccc cgcagtggat
    16081 aatgagtttc agcaatggct ttcccaaatc aatcaggtat gcggaaattt taccggacgc
    16141 ctgctgactg agcgttacac gggtgtactg gacacgcatt ttgccaaagg actaaagctg
    16201 agtaccgtga caaccaacgg ggtgaattta tcccgcacct ggcaggaaat aaaaggcagc
    16261 gacgatgcct ggttttacac cgtttttcag cttagtggtc aggcaataat ggagcaggat
    16321 gagcgtcagg tgcagattgg cgctggcgat attacgttac tcgatgcctc acgcccctgt
    16381 tcgctttact ggcaggagtc ttctaaacag atttcattac ttttgccacg cactctgctg
    16441 gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa gactggacgc tgacttaccc
    16501 atggtgcaac tcagtcatcg cctgttacag gagagcatga ataatccggc actttctgaa
    16561 acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc ttcgcccggt acttcatcag
    16621 cgggaatctg tccaacctcg ccgtgaacgt cagtttcaaa aagtggttac gttgatagac
    16681 gataatattc gcgaagagat attacgcccg gagtggatag ccggagagac aggtatgtca
    16741 gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg tagtcgcgca atatattcgt
    16801 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
    16861 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
    16921 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgctaact aattcgcgtt
    16981 gtgtgtactg caacgccaat tagttaaaac agatagttga ctatgcattt ttcagggcga
    17041 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
    17101 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
    17161 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
    17221 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
    17281 tttacgttcg gcattaattt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
    17341 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
    17401 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
    17461 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
    17521 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
    17581 taggtgcagc gacttcaatg cgaggcgtac agccaaccag cataaaagat gacgtcaacg
    17641 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
    17701 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
    17761 ccacagcgta aaaatattct cttcatgata gtaattaagg tttaccgtac cgcttttacc
    17821 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
    17881 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
    17941 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
    18001 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
    18061 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
    18121 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgct gcggcatacg
    18181 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
    18241 tggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
    18301 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
    18361 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
    18421 catccaggca ctaccgcctt tcaacacgcc gtgccctccc gcgcggaatc cttgttcagg
    18481 cgcagcagaa aatgcaacct gagcatacag ttcaccatcg cgtaagttca tcttccagtc
    18541 gggtggcacc agcggctgga atacagtcag tgattgttta ggccaccagg cgttaccgcg
    18601 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
    18661 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
    18721 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
    18781 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
    18841 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agagatcggt
    18901 cagcgtaatc gtgctgtcat gccactcacc ttttcctgcg acatcccagg tggcattcat
    18961 cggtgtaaaa ctcccttttc cccagtagcg ccactgccag cggccagcgt cagggagaaa
    19021 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatttt catgcgcctg
    19081 caagatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
    19141 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
    19201 gcctggctca aaggccaacg ttgggtcagt cagacttccg cttagctgcg caggtaaacg
    19261 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agttgcagag gcagctgact
    19321 gttatccatg cttaattttc ctgggccaaa attaagcacc gcgttgcctt tacccgcttg
    19381 cccctgggtc agcacgctca gtcgtccgct gaccagagcg ttctcgagcc ctgcctgcca
    19441 gttgtcgact ttaacaccca gtcggccact caaaggaaaa cctgcatacg gccagctcca
    19501 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
    19561 atcgccgtta tcccgtgcca gcacgatcaa ttgcccgcta ttttcctgcc agtccagctc
    19621 ggcatccacc agtgaaggct cctgcggcaa gtttaacgtt gcagtagcat gaccacttac
    19681 aggaagtcca tccggcacta gcggcatagt aaattccccc accagtttaa ccggcggctg
    19741 attttcaaac gcgacgacat ccagttcgct gactgtaagt tgttgccctt tcagctgacc
    19801 ttgaaattta actttttcgc cctgataacg cagttgctgg atatcagagg ttaatgcgag
    19861 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
    19921 ggtgttaggc agcatggact gccattgcgc gaggtttttt ggagcggctg gcgattgctc
    19981 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accgtgccga cgttcaataa
    20041 ccagcggcta ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
    20101 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
    20161 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
    20221 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
    20281 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
    20341 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcatagttgt
    20401 tgatgaattt ttcaatatcg ccatgacttt caattatatt ttaaattttg taaaatattt
    20461 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
    20521 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
    20581 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaacggccaa tggctgcgaa
    20641 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
    20701 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
    20761 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
    20821 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
    20881 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
    20941 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
    21001 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
    21061 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
    21121 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
    21181 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
    21241 ctgaaaactc agaaaattgg ttcgttgggt atggacgtgt atgagaacga acgcgatctg
    21301 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
    21361 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
    21421 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaagcgaaac ctgcccgaac
    21481 gaactggttt aatcttgccg ctcccctgca ttccagggga gctgattcag ataatcccca
    21541 atgacctttc atcctctatt cttaaaatag tcctgagtca gaaactgtaa ttgagaacca
    21601 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
    21661 atgacaaaat tgctgtaacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
    21721 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
    21781 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
    21841 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
    21901 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
    21961 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
    22021 attaatagct gccacagctc ccggcggcaa gtgactgttc actacagcgt ttgccgttgg
    22081 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
    22141 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
    22201 cgtgcgccgc agtcgcctgc tgaacaggct cactactaca cgccgacaac aataaagcgg
    22261 cacaccctac ccaaaacgct gctcgcatct cttcttcctc ttatcttcaa gccaaacgac
    22321 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
    22381 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
    22441 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaaag
    22501 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
    22561 tatgattact attgacggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
    22621 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
    22681 cggaaacggc ttgaagaacg tttggggaga cacaccacgc gtggttgaaa tgcagtcgga
    22741 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
    22801 tacgtcatcg cagggtttgc tgctgatgat cccgacgctg tacaagctgg caggcgaact
    22861 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
    22921 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
    22981 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
    23041 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca ataaaattgt
    23101 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
    23161 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
    23221 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
    23281 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
    23341 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
    23401 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
    23461 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
    23521 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
    23581 gatgaccgca ctggcagaag cctttaataa tggcgagcgt gaaaccctgc cccgtgtcat
    23641 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
    23701 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
    23761 gactaatctg tcactgccgt tgccggaaaa caccctgcca aactcggcga aactggaagc
    23821 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
    23881 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaggc
    23941 gggcggcctg acggtttctc accttcgagt gagcgaacag ccgattcgtt ccgcttatct
    24001 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
    24061 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acaccgtaca gcgcagatga
    24121 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
    24181 ctatgtgatt aacgcggcga aaatcgcccg cgaatgtggc ctggcggccc gtattaatac
    24241 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
    24301 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
    24361 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
    24421 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
    24481 aaccgtaacc gctgcgatgc tcgccgggct tggtgatgcc ctccccgttt cggcgctgcc
    24541 gccagacggc acctggccga tgggcactac gcgctgggaa aaacgcaata tcgccgaaga
    24601 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg ccgcttgccc
    24661 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
    24721 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
    24781 ggcaccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
    24841 tcagaatcca gagattaaag ccatcaatat gatgtctcgc ctggaacatg tcgaagaaga
    24901 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
    24961 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattcag gtgcttgctc
    25021 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
    25081 gatcgctaac gccactggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
    25141 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctgtttg aagataatgc
    25201 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
    25261 gctggatcaa tttgccgata aaatcccggc ggaattactg acagcgttga aatcagacgc
    25321 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
    25381 cgaagcacat gaactgctgc gtgacgcaga tgcactggtg gaaaaatcaa tctggctgat
    25441 tggtggcgac ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
    25501 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
    25561 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
    25621 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
    25681 ttctctcggc gcgcagctca accagacggt gaaagcgatt caggaagcgg aagcgtatcc
    25741 ggggccatcg ctgatcatcg cttatagccc atgtgaagag catggttacg atctggcact
    25801 cagccacgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
    25861 tccgcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
    25921 agcactggaa gaaacgttac ttcacgagca acgtttccgt cggctgaatt cgcagcagcc
    25981 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
    26041 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
    26101 gattatccaa ttctaaaaaa aaagcccgga cgactgttcg ggcttgtctt tttatatgtt
    26161 ggaaaatcag tggcaatgca atggcccaac agaaaataaa atctgtgaca ataaaggc
//
LOCUS       DAZKIV010000055        25787 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZKIV010000055 DAZKIV010000000
VERSION     DAZKIV010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.055"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..991
                     /gene="ybhD"
                     /locus_tag="ACHOT6_002240"
     CDS             38..991
                     /gene="ybhD"
                     /locus_tag="ACHOT6_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9947456.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWANIVERQRHALQEIS"
     gene            complement(1032..2027)
                     /gene="pgl"
                     /locus_tag="ACHOT6_002241"
     CDS             complement(1032..2027)
                     /gene="pgl"
                     /locus_tag="ACHOT6_002241"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFP9947457.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            2182..3000
                     /gene="ybhA"
                     /locus_tag="ACHOT6_002242"
     CDS             2182..3000
                     /gene="ybhA"
                     /locus_tag="ACHOT6_002242"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFP9947458.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(3001..4059)
                     /gene="modC"
                     /locus_tag="ACHOT6_002243"
     CDS             complement(3001..4059)
                     /gene="modC"
                     /locus_tag="ACHOT6_002243"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFP9947459.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(4062..4751)
                     /gene="modB"
                     /locus_tag="ACHOT6_002244"
     CDS             complement(4062..4751)
                     /gene="modB"
                     /locus_tag="ACHOT6_002244"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFP9947460.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(4751..5524)
                     /gene="modA"
                     /locus_tag="ACHOT6_002245"
     CDS             complement(4751..5524)
                     /gene="modA"
                     /locus_tag="ACHOT6_002245"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9947461.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(5690..5839)
                     /gene="acrZ"
                     /locus_tag="ACHOT6_002246"
     CDS             complement(5690..5839)
                     /gene="acrZ"
                     /locus_tag="ACHOT6_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFP9947462.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            5968..6756
                     /gene="modE"
                     /locus_tag="ACHOT6_002247"
     CDS             5968..6756
                     /gene="modE"
                     /locus_tag="ACHOT6_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFP9947463.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            6824..8296
                     /gene="modF"
                     /locus_tag="ACHOT6_002248"
     CDS             6824..8296
                     /gene="modF"
                     /locus_tag="ACHOT6_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFP9947464.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTIFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            8557..9573
                     /gene="galE"
                     /locus_tag="ACHOT6_002249"
     CDS             8557..9573
                     /gene="galE"
                     /locus_tag="ACHOT6_002249"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415280.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFP9947465.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGNSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            9583..10629
                     /gene="galT"
                     /locus_tag="ACHOT6_002250"
     CDS             9583..10629
                     /gene="galT"
                     /locus_tag="ACHOT6_002250"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFP9947466.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            10633..11781
                     /gene="galK"
                     /locus_tag="ACHOT6_002251"
     CDS             10633..11781
                     /gene="galK"
                     /locus_tag="ACHOT6_002251"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFP9947467.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMEESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            11775..12815
                     /gene="galM"
                     /locus_tag="ACHOT6_002252"
     CDS             11775..12815
                     /gene="galM"
                     /locus_tag="ACHOT6_002252"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFP9947468.1"
                     /translation="MLNETPALTPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            13018..13770
                     /gene="gpmA"
                     /locus_tag="ACHOT6_002253"
     CDS             13018..13770
                     /gene="gpmA"
                     /locus_tag="ACHOT6_002253"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFP9947469.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(13937..14989)
                     /gene="aroG"
                     /locus_tag="ACHOT6_002254"
     CDS             complement(13937..14989)
                     /gene="aroG"
                     /locus_tag="ACHOT6_002254"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFP9947470.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            15305..15685
                     /gene="ybgS"
                     /locus_tag="ACHOT6_002255"
     CDS             15305..15685
                     /gene="ybgS"
                     /locus_tag="ACHOT6_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFP9947471.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            15799..16740
                     /gene="zitB"
                     /locus_tag="ACHOT6_002256"
     CDS             15799..16740
                     /gene="zitB"
                     /locus_tag="ACHOT6_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFP9947472.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(16737..17456)
                     /gene="pnuC"
                     /locus_tag="ACHOT6_002257"
     CDS             complement(16737..17456)
                     /gene="pnuC"
                     /locus_tag="ACHOT6_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFP9947473.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(17494..18537)
                     /gene="nadA"
                     /locus_tag="ACHOT6_002258"
     CDS             complement(17494..18537)
                     /gene="nadA"
                     /locus_tag="ACHOT6_002258"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFP9947474.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKVYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSISV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDSAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(18786..18911)
                     /locus_tag="ACHOT6_002259"
     ncRNA           complement(18786..18911)
                     /ncRNA_class="other"
                     /locus_tag="ACHOT6_002259"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(18942..19017)
                     /locus_tag="ACHOT6_002260"
     tRNA            complement(18942..19017)
                     /locus_tag="ACHOT6_002260"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18982..18984),aa:Lys,seq:ttt)
     gene            complement(19051..19126)
                     /locus_tag="ACHOT6_002261"
     tRNA            complement(19051..19126)
                     /locus_tag="ACHOT6_002261"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19091..19093),aa:Lys,seq:ttt)
     gene            complement(19273..19348)
                     /locus_tag="ACHOT6_002262"
     tRNA            complement(19273..19348)
                     /locus_tag="ACHOT6_002262"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19313..19315),aa:Lys,seq:ttt)
     gene            complement(19352..19427)
                     /locus_tag="ACHOT6_002263"
     tRNA            complement(19352..19427)
                     /locus_tag="ACHOT6_002263"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19392..19394),aa:Val,seq:tac)
     gene            complement(19577..19652)
                     /locus_tag="ACHOT6_002264"
     tRNA            complement(19577..19652)
                     /locus_tag="ACHOT6_002264"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19617..19619),aa:Lys,seq:ttt)
     gene            complement(19655..19730)
                     /locus_tag="ACHOT6_002265"
     tRNA            complement(19655..19730)
                     /locus_tag="ACHOT6_002265"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19695..19697),aa:Val,seq:tac)
     gene            complement(19866..19941)
                     /locus_tag="ACHOT6_002266"
     tRNA            complement(19866..19941)
                     /locus_tag="ACHOT6_002266"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19906..19908),aa:Lys,seq:ttt)
     gene            complement(20106..20897)
                     /gene="cpoB"
                     /locus_tag="ACHOT6_002267"
     CDS             complement(20106..20897)
                     /gene="cpoB"
                     /locus_tag="ACHOT6_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFP9947475.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(20907..21428)
                     /gene="pal"
                     /locus_tag="ACHOT6_002268"
     CDS             complement(20907..21428)
                     /gene="pal"
                     /locus_tag="ACHOT6_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFP9947476.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(21463..22755)
                     /gene="tolB"
                     /locus_tag="ACHOT6_002269"
     CDS             complement(21463..22755)
                     /gene="tolB"
                     /locus_tag="ACHOT6_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFP9947477.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSEQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(22888..24099)
                     /gene="tolA"
                     /locus_tag="ACHOT6_002270"
     CDS             complement(22888..24099)
                     /gene="tolA"
                     /locus_tag="ACHOT6_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFP9947478.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAAAKAAAEA
                     DDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKF
                     YDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYE
                     VFKNAPLDFKP"
     gene            complement(24164..24592)
                     /gene="tolR"
                     /locus_tag="ACHOT6_002271"
     CDS             complement(24164..24592)
                     /gene="tolR"
                     /locus_tag="ACHOT6_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFP9947479.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(24596..25288)
                     /gene="tolQ"
                     /locus_tag="ACHOT6_002272"
     CDS             complement(24596..25288)
                     /gene="tolQ"
                     /locus_tag="ACHOT6_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFP9947480.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(25285..25689)
                     /gene="ybgC"
                     /locus_tag="ACHOT6_002273"
     CDS             complement(25285..25689)
                     /gene="ybgC"
                     /locus_tag="ACHOT6_002273"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFP9947481.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
BASE COUNT         6293 a         6816 c         6365 g         6313 t
ORIGIN      
        1 ccacccaaaa tcgtcgcttc cattttccgg agtaataatg aagcatgaat tatcaagtat
       61 gaaggcattt gtcatactgg cagagtccag ttcatttaac aatgccgcta aattactcaa
      121 tattacgcaa cctgcattaa cgcgaagaat aaaaaagatg gaggaagatt tacatattca
      181 actatttgag cgtacaactc gcaaggttac gttaacaaaa gcaggaaaaa ggttgctccc
      241 ggaggcgcgg gaattaataa agaaattcga tgaaacgctt tttaatattc gtgatatgaa
      301 tgcttatcat cgtggtatgg tgacgttagc gtgtattccg accgcagtat tttatttttt
      361 accgctggca atcggtaaat ttaatgagct gtatcccaat attaaagtgc ggattctgga
      421 acaaggcacg aataattgca tggaatcggt gctgtgtaac gaatcagact ttggcataaa
      481 catgaacaac gtcacaaatt catccattga ttttacccca ctggtcaacg agccattcgt
      541 gctggcctgt cggcgtgacc atccattggc caaaaagcag cttgtagaat ggcaggagtt
      601 agtgggttac aaaatgattg gcgtacgttc ctccagcggc aaccgattgt tgatagaaca
      661 acagctagcc gacaagccct ggaagctgga ctggttttac gaagtgcgtc atctttcgac
      721 gtcattagga ctagtggagg caggactggg gatttcggcg cttcctggtc tggcaatgcc
      781 ccatgcgccc tattcttcga ttattggtat cccactggtg gaacccgtta tacgtcggac
      841 attggggatt attcgccgca aagatgccgt gctttctccg gcagctgaac gcttttttgc
      901 cttgctgatt aatttgtgga ctgacgataa agacaatttg tgggccaata tcgtcgaacg
      961 ccagagacat gcgttacagg agataagtta atacaccggc gcaggagaga ctgcgccggt
     1021 taaatcagcg gttagtgtgc gttaaccacc acccacattg gtccctgccc gaccgcataa
     1081 cggccttttt catgcagtag tccctgctcg ccaacaattt cgtataccga gatgtggtga
     1141 gatttttgcc cggcggcaat cagatacttg ccgctgtgat caacattgaa gccgcgcggc
     1201 tgggtttccg ttggctggaa gccttcttta ctcaacacgc tgccatcttc cgaaacgctg
     1261 aaaacggtta tcaggctggc ggtacggtcg caggcgtata aatggcgacc atccggggtg
     1321 atatgaatat cagccgccca acgggtgtcg gagaagtttt ccggcatcat atccagcgtc
     1381 tggacacatt cgatattacc gtgcggatct ttcagttccc agacatccac tgagctgttt
     1441 aactcattga cgcaatacgc atattgttcg tttggatgga ataccatatg acgcgggccg
     1501 gccccttcaa cggtggtcac ttccgcaggg tcctgcgcca cgagatgacc atcatcgctg
     1561 accgtaaaca ggcaaatgcg atcctgcttt aatgccggaa cccacagcgt acggttgtcc
     1621 ggtgagatat tggcggaatg gcaaccgtcc agcccctcga ccacatcgac gacgcccact
     1681 ggcaggccat cttccagacg cgttacgctc acgttacccg cattgtaaga acctacaaag
     1741 acaaactgcc cctggtgatc ggtggaaata tgcgtcggac tacccggcag cgcagactct
     1801 gcggcaaagg tcagtgcgcc atcgtccggg gcaatacgat acgccaggac gcgaaactca
     1861 gggcgaacac caacatagag ataacgtttg tccgggctga ccaccatcgg ctgcacctgc
     1921 cccggcacat cgacaacctg tgtcagcgtc agtgcgcctt catgattcag gttccagacg
     1981 tgaatttgct ggctctcagg gctggcgata taaactgttt gcttcatgaa tgctcctttg
     2041 catttagcaa ctgactgtaa cagctaaaat tagtcgcttt tggcggtgaa tgctgaatta
     2101 aaacgcagaa ttttgtccga tagtggtatc ggtgtaccat tcgcgcagac gaaaattctt
     2161 aaccttaatc tggaatacgc catgaccaca cgcgtgattg ctctcgactt agacggcacc
     2221 ttattgaccc cgaaaaagac cctgcttcct tcatcgatag aagccctggc ccgcgctcgc
     2281 gaagcaggct atcaattaat catcgtcaca ggtcgccatc acgtcgctat tcatcctttt
     2341 tatcaggcgc tggcgctgga tacacctgct atttgctgta atggcaccta tttgtatgat
     2401 tatcatgcaa aaaccgtgct ggaagcggac ccaatgcccg ttaataaagc cctgcaactc
     2461 attgagatgc tgaatgaaca ccacattcac ggtctgatgt atgtggatga tgcaatggtc
     2521 tatgagcacc cgaccgggca tgtcattcgc acgtctaact gggcgcaaac cctgccgccg
     2581 gaacagcgcc cgactttcac acaagtcgct tctctggctg aaacggcgca acaagttaac
     2641 gccgtatgga agttcgccct gacgcacgat gacctgccgc aattgcagca ttttggtaag
     2701 catgtcgaac atgaactggg actggagtgt gaatggtcct ggcacgatca ggttgatatt
     2761 gcacgcggcg gcaacagcaa aggtaaacgt ttgacgaaat gggttgaggc gcaaggctgg
     2821 tcgatggaaa acgtcgtggc gttcggcgat aactttaatg atatcagtat gctggaggcc
     2881 gctggtacag gcgtggcgat gggcaacgcc gatgacgcgg taaaagcgcg cgccaacatt
     2941 gtgattggtg ataacaccac cgacagcatt gcccagttca tttatagcca cctgatttaa
     3001 tcaggcggtt atcgacacac ttttaatttg cgcgtacagc cacaggccag gtttgatcac
     3061 cagttcatcc ctggcccacg ggctgatacg cgcccacagc gttttacctc cgacttccag
     3121 ttccacttcc acctggccgt tgtcgtcata actattaaca acttttgccc gcaatacgtt
     3181 acgaatgctg gtttgctgcg gcggttgtaa aaccagagaa acatcggaag cctgaatgcg
     3241 gatacgtagc gcagcttgca gcggttcgtc cagcttattg acccacaaat gctgatcgcc
     3301 cagcgccagc gcagtcatcg cgtaatgcgg atgatgctca agtaccgtca ctttcagaat
     3361 gctactttgt tgctctttcg gcagccacgg attcatcacg ctactacccc agacttcttc
     3421 cagtgcgcca aaggctttca cctgaccgtt ttccagtacc atcactctgt ctgccagatg
     3481 gaggatctca tccagcgaat ggctgacata caacatcggg atgttgattt cccgcgtcag
     3541 acgttgcaga taaggcaaca gttcgcgttt acgcggaata tccagtgacg ccagcggttc
     3601 atccagcaac agcaattctg gtgccgtcag caaagcccgc ccaatcgcta cgcgctgttt
     3661 ttcgcctccg gacaggctgc ctggtaaacg gtcaagcaac ggttcaatgc ctaaaagcgc
     3721 caccagctta tcgaactgat cgaccatgct tttcgccatg ccgtagcgca gattgccacg
     3781 cactttgtaa tgcggaaaca gccgcgcatc ctgaaaaaca tagccaacgc gacgcttttc
     3841 tggcgtcagg cagatacctt tttcggcatc atttagtacc cgaccattga ggacaatcct
     3901 ccctttttgc gggcgcgtca gtccactgat ggcgttaatc agcgaagttt ttccggcacc
     3961 ggagacgcca aagatagcag tgatgccatt ggcgggcagc gtttcattaa tggtcaggca
     4021 atggttgccc aaggtctggg aaaaattcag ttccagcatg attagcgccc cgcccgttca
     4081 cggctgattc tggccagcca ttctgaaatc aacagggaga tcatcgccag cgcaatagaa
     4141 ataatgcaca gtctcgccgc gccactttcc ccgccggggg tctggatcag ggtatacatg
     4201 gcagaaggaa tggttcgcgt ttcaccagga atgttcgaca caaaggtgat ggttgcacca
     4261 aactcaccga gagaacgagc aaaagccagt accgtaccaa caataattcc cggtaaggtc
     4321 agcggtaacg tgatagtaaa gaaaacgcgc cagcgcccgg cccccagtgt tcttgcggcc
     4381 tgttcgagtt tgacatcaac cccttccagc gccagacgaa ttgcccgcac catcagcgga
     4441 aacgacatga cggcggcagc gagaaccgcg ccgcgccagc taaaggcgaa ggtaatacca
     4501 aaccagtcat acagacgttc accgataaat ccgcgccgtc ccatcgaaac taataataag
     4561 taaccgacga ccacgggcgg taacaccagc ggtagatgca gtacgctgtc gagcagagct
     4621 ttgcccggaa acgtgcaacg caccagtaac caggcaaaaa agatcccaaa cggcaggcta
     4681 aacagcacag ccagggaaga aactttcagg cttaataaaa ctgcctgcca ttctggatcg
     4741 gtcagtatca ttacttggtt gtaaatccgt aacgtttaaa gatttccgca gcctgcggcc
     4801 ctttcaaata atcataaaat gctttcactg tcgcattgtt atgcccttcc acaacagcaa
     4861 ccggatattc cactttttta tgtgaatctt ccgggaaggt ggcaaccact tttacccctt
     4921 tgctggcaac tgcgtcagaa ccgtaaacaa tgcccaacgg cgcttcattg cgttcgacca
     4981 acgccagcgc gccacgaaca tcttccgccg gagccagttt cggagagagc gtatcccatg
     5041 cgcccagttt ttgcagtgct tctttcgcat aaatgccagc agggacatgt tccggatcgc
     5101 caaccgcgag gcgaccgcca ttcagcagcg aagtccagtt ggttttgctg tcgatggtga
     5161 aatctttctg ctcgctggct ttcggtgcta caacgaccag gctattgccg agcagtgtct
     5221 ggcgcgaagc cgtatcgatc gcttttttat caaccgcata atccatccat ttctgatcgg
     5281 cagaaataaa cagatccgca ggcgcacccg cttcaatctg acgggccaaa gtagatgacg
     5341 aagcgaaaga agaaaccaca tccacgcctt tttctttttt atactgcgta gcaatgtcct
     5401 gcattgcgtt agtcagtgat gcggcggcga acaccgtgat tttcccttca tctgccagtg
     5461 tattgccagc aacagcgaaa gagagtgccg ccccggcaaa caagttcaac catttacgag
     5521 ccattggtaa ccccttaatg taacgttata taggtgacaa tataacggct agctcagaat
     5581 tttcccagca gttattgcta acctttgtga ggtagataag aaaaaatatc ggctggaaaa
     5641 gcaggaagtt gagaaaaaga aatgcccgac taagcgggca ttcagggaat caatgatttt
     5701 gtccgggctg gtctttttta ccaacaccag aaaagatgtt gaatacttca ccaagaccgt
     5761 aaatcagacc caggatgatg gccatcacga caggtaccat gattacggcg aataccagac
     5821 tttttaataa ctctaacatg gtcaactcca gatatagtca tgagactatt ctaaccgcta
     5881 agcacagaaa agcactcccc ttttgtgcgg tcagctttgc gtggcgttcg ttttccgtca
     5941 caataagact tttgccagga cattgttatg caggccgaaa tccttctcac ccttaagctc
     6001 caacaaaaat tattcgccga cccgcgccgg atttcgctac taaaacacat tgcgctttcc
     6061 ggttccatta gccagggagc gaaagatgcc ggtatcagct ataaaagcgc ctgggatgcc
     6121 attaacgaga tgaatcagtt aagtgagcat attctggtcg agcgcgcaac aggcggtaaa
     6181 ggtggcggcg gcgcagtact gacccgctat ggtcagcgac tgattcagct ctatgactta
     6241 ctggcgcaaa tccagcaaaa agcctttgat gtgttaagtg acgatgacgc cctgccgctg
     6301 aacagcctgc tggccgcgat ctcacgtttt tcactgcaaa ccagcgcccg taaccagtgg
     6361 ttcggtacca tcaccgcccg cgatcatgat gacgttcaac agcatgttga tgtcttactg
     6421 gctgacggaa aaacacgcct gaaagtcgca attaccgcac aaagcggcgc acgtctgggg
     6481 ctggatgaag gcaaagaagt gttgatattg ctaaaagcgc cgtgggtagg cattactcag
     6541 gacgaagcgg tcgcacaaaa cgccgacaac caattaccgg gtattattag tcatattgag
     6601 cgcggcgcag agcagtgcga agtattaatg gcgctacccg acgggcaaac actgtgcgcc
     6661 acagtgccgg taaatgaagc gacttctctt cagcaagggc agaatgtcac ggcctacttt
     6721 aatgccgaca gcgtgattat cgccacgctg tgctaagcgt gttgacaatt tgttatgaaa
     6781 cacgtatccc tgtcagtaat cgctgcacaa aatgggatat aaaatgtcat cgttgcaaat
     6841 tttgcaaggc acgtttcgtc ttagcgacac aaaaacgctg caattgcctc agctaacgtt
     6901 aaacgcgggt gatagttggg cgtttgtcgg ttcgaatgga agcgggaaat cggccctggc
     6961 ccgcgcgctg gcgggggaac ttccgctttt gaaaggtgaa cggcaaagcc agttttccca
     7021 catcactcgt ctctccttcg agcaattgca aaaactcgtc agcgacgaat ggcagcggaa
     7081 taacaccgat atgctcggcc ctggcgaaga tgacaccgga cgcactacgg ctgagatcat
     7141 tcaggatgaa gtaaaggatg caccgcgttg catgcaactg gcgcagcagt tcggtattac
     7201 cgccctcctc gaccgacgct ttaaatacct ttccactggc gagacgcgaa aaaccctgct
     7261 gtgtcaggcg ctgatgtcgg agcctgactt gttgattctt gatgagccgt tcgatggcct
     7321 ggatgttgcc tcacgtcagc agctggctga gcgactcgcc tcgttacatc agtccggtat
     7381 tactctggta ctggtgctca atcgcttcga tgagatcccg gagtttgtcc agtttgctgg
     7441 cgtgctggcg gattgcacgt tagcggaaac tggcgctaaa gaggaactgc tccaacaagc
     7501 actcgtcgcg caactggcgc atagtgaaca gcttgaaggt gtgcaactgc cggagccgga
     7561 tgaaccttca gcacgtcacg ccttacccgc caacgaaccg cgcattgtgc tgaacaatgg
     7621 cgtggtttct tataacgatc gccccattct taataacctt agctggcagg tgaatccagg
     7681 tgaacactgg caaattgtcg ggccaaatgg tgcaggaaaa tcgacgttat taagcctggt
     7741 tactggcgat catccgcaag gttacagcaa cgatttgacg attttcggac gacgtcgcgg
     7801 cagcggcgaa accatctggg atatcaaaaa gcatatcggt tacgtcagca gtagtttgca
     7861 tctggattac cgggtcagca ctaccgtgcg taatgtgatt ctttctggct attttgattc
     7921 gattggcatt tatcaggccg tttcggatcg ccagcaaaaa ctggtgcagc agtggctgga
     7981 tattctcggc attgataaac gcacggctga cgctccgttc catagtcttt cctggggaca
     8041 gcagcgtctg gcgctgatcg tccgcgcgct ggtgaaacat ccgacgttgc ttattctcga
     8101 tgaaccacta caggggcttg atccgctcaa tcgccagctt atccgccgtt ttgttgatgt
     8161 gctgattagc gaaggtgaaa cgcaattgtt gtttgtttcg caccacgctg aagatgcgcc
     8221 tgcctgtatt acccatcgcc ttgagttcgt gccggacggt ggactctatc gctatgtgct
     8281 gacaaaaata tattgagtcg gtagtgctga ccttgccgga ggcagcctaa gcgccctctc
     8341 cggccaacgg ttcgacgcat gcaggcatga aaccgcttct tttttcagat aaaaagcgca
     8401 atcattcata aaccctctgt tttataatca cttaatcgcg cataaaaaac ggctaaattc
     8461 ttgtgtaaac gattccacta atttattcca tgtcacactt ttcgcatctt tgttatgcta
     8521 tggttatttc ataccataag cctaatggag cgaattatga gagttctggt taccggtggt
     8581 agcggttaca ttggaagtca tacctgcgtg caattactgc aaaacggtca tgatgtcatc
     8641 attcttgata acctctgtaa cagtaagcgc agcgtactgc ctgttatcga gcgtttaggc
     8701 ggcaaacatc caacgtttgt tgaaggcgat attcgtaacg aagcgttgat gaccgagata
     8761 ctgcacgatc acgctatcga caccgtgatc cacttcgccg ggctgaaagc cgttggcgaa
     8821 tcggtacaaa aaccgctgga atattacgac aacaatgtca acggcactct gcgcctgatt
     8881 agcgccatgc gcgccgctaa cgtcaaaaac tttattttta gctcctccgc caccgtttat
     8941 ggcgatcagc ccaaaattcc atacgttgaa agcttcccga ccggcacacc gcaaagccct
     9001 tacggcaaaa gcaagctgat ggtggaacag atcctcaccg atctgcaaaa agcccagccg
     9061 gactggagca ttgccctgct gcgctacttc aacccggttg gcgcgcatcc gtcgggcgat
     9121 atgggcgaag atccgcaagg cattccgaat aacctgatgc catacatcgc ccaggttgct
     9181 gtaggccgtc gcgactcgct ggcgattttt ggtaacgatt atccgaccga agatggtact
     9241 ggcgtacgcg attacatcca cgtaatggat ctggcggacg gtcacgtcgt ggcgatggaa
     9301 aaactggcga acaagccagg cgtacacatc tacaacctcg gcgctggcgt aggcaacagc
     9361 gtgctggacg tggttaatgc cttcagcaaa gcctgcggca aaccggttaa ttatcatttt
     9421 gcaccgcgtc gcgagggcga ccttccggcc tactgggcgg acgccagcaa agccgaccgt
     9481 gaactgaact ggcgcgtaac gcgcacactc gatgaaatgg cgcaggacac ctggcactgg
     9541 cagtcacgcc atccacaggg atatcccgat taaggaacga ccatgacgca atttaatccc
     9601 gttgatcatc cacatcgccg ctacaacccg ctcaccgggc aatggattct ggtttcaccg
     9661 caccgcgcta agcgcccctg gcagggggcg caggaaacgc cagccaaaca ggtgttacct
     9721 gcgcacgatc cagattgctt cctctgcgca ggtaatgtgc gggtgacagg cgataaaaac
     9781 cccgattaca ccgggactta cgttttcact aatgactttg cggccttgat gtctgacacg
     9841 ccagatgcgc cagaaagcaa cgatcctttg atgcgttgcc agagcgcgcg cggtaccagc
     9901 cgggtgatct gcttttcacc ggatcacagt aaaacgttgc cagaactgag cgttgcggca
     9961 ttgacggaaa tcgtcaaaac ctggcaggag caaaccgcag agctgggaaa aacgtacccg
    10021 tgggtgcagg tctttgaaaa caaaggtgcg gcgatgggct gctctaaccc gcatccgcac
    10081 ggacagattt gggcaaatag cttcctgcct aacgaagctg agcgcgaaga ccgcctgcaa
    10141 aaagaatatt ttgccgaaca gaaatcacca atgctggtgg attatgttca gcgcgagctg
    10201 gcagacggta gccgtaccgt tgtcgaaacc gaacactggt tagccgttgt gccttactgg
    10261 gctgcctggc cgttcgaaac gctactgctg cccaaagccc acgttttacg gatcaccgat
    10321 ttgaccgacg cccagcgcag cgatctggcg ctggcgttga aaaagctgac cagtcgttat
    10381 gacaacctct tccagtgctc cttcccctac tctatgggct ggcacggcgc accatttaat
    10441 ggcgaagaga atcaacactg gcagctgcac gcgcactttt atccgcctct gctgcgctcc
    10501 gccaccgtac gtaaatttat ggttggttat gaaatgctgg cagagaccca gcgagacctg
    10561 accgcagaac aggcagcaga gcgtttgcgc gcagtcagcg atatccattt tcgcgaatcc
    10621 ggagtgtaag aaatgagtct gaaagaaaaa acacaatctc tgtttgccaa cgcatttggc
    10681 taccctgcca ctcacaccat tcaggcgcct ggccgcgtga atttgattgg tgaacacacg
    10741 gactacaacg acggtttcgt tctgccctgc gcgattgatt atcaaaccgt gatcagctgt
    10801 gcaccacgcg atgaccgtaa agttcgcgtg atggcagccg attatgaaaa tcagctcgac
    10861 gagttttccc tcaatgcgcc cattgtcgcg catgaaaact atcaatgggc gaactacgtt
    10921 cgtggcgtgg tgaaacatct gcaactgcgt aacaacagct tcggcggtgt ggacatggtg
    10981 atcagcggca atgtgccgca gggtgccggg ttaagttctt ccgcttcact ggaagtcgcg
    11041 gtcggaaccg tattgcagca gctttatcat ctgccgctgg acggcgcaca aatcgcgctt
    11101 aacggtcagg aagcagaaaa ccagtttgtt ggctgtaact gcgggatcat ggatcagcta
    11161 atttccgcac tcggcaagaa agatcatgcc ttgctgattg actgtcgctc actggggacc
    11221 aaagcagttt ccatgccgaa aggtgtggct gtcgtcatca tcaacagtaa cttcaaacgt
    11281 accctggttg gcagcgaata caacacccgt cgtgaacagt gcgaaaccgg tgcgcgtttc
    11341 ttccagcagc cagccctgcg cgatgtcacc attgaagagt tcaacgctgt tgcacatgag
    11401 ctggacccaa tcgtggcgaa acgcgtgcgg catatcctga ctgaaaacgc ccgcaccgtt
    11461 gaagctgcca gcgcgctgga gcagggcgac ctgaaacgta tgggcgagtt gatggaggag
    11521 tctcatgcct ctatgcgcga tgatttcgaa atcaccgtgc cgcaaattga cactctggta
    11581 gaaatcgtca aagctgtgat tggcgacaaa ggtggcgtac gcatgaccgg cggcggattt
    11641 ggcggctgta tcgtcgcgtt gatcccggaa gagctggtgc ctgccgtaca gcaagctgtc
    11701 gctgaacaat atgaagcaaa aacaggtatt aaagagactt tttacgtttg taaaccatca
    11761 caaggagcag gacagtgctg aacgaaactc ccgcactgac acccgatggt cagccgtacc
    11821 gactgttaac tttgcgtaac aacgcaggga tggtagtcac gctgatggac tggggtgcga
    11881 ctttactttc agcccgtatt ccgctttccg atggcagcgt ccgcgaggcg ctgctcggct
    11941 gtgccagccc ggaatgctat caggatcagg ccgcgtttct gggggcctct attggtcgtt
    12001 atgccaaccg catcgccaat agccgttata cctttgacgg tgaaaccgtg accctttcgc
    12061 caagtcaggg cgttaaccag ctgcacggcg gcccggaagg gttcgacaaa cgtcgctggc
    12121 agattgtgaa ccagaacgat cgtcaggtgc tgtttgccct gagttcagat gatggcgatc
    12181 agggcttccc gggtaatctc ggcgcaacgg tgcaatatcg tctgaccgac gataaccgta
    12241 tctccattac ttatcgcgcc acagttgata aaccttgccc ggtgaatatg actaatcacg
    12301 tctatttcaa tcttgacggc gagcagtctg acgtgcgcaa tcacaagttg cagattctgg
    12361 cggacgaata tcttccggtt gatgaaggcg gcattccgca cgacggcttg aaatctgtcg
    12421 ccggaacgtc ttttgatttc cgcagcgcca aaatcatcgc cagtgagttt cttgccgacg
    12481 acgatcagcg caaagtgaaa ggttacgatc acgcattctt gttacaggcc aaaggcgatg
    12541 gcaagaaagt ggcggcgcat gtctggtcag cagatgaaaa attgcagctg aaggtctaca
    12601 ccaccgctcc ggctcttcaa ttctactccg gcaacttcct cggcggcaca ccgtcgcggg
    12661 gaaccgaacc ttacgccgac tggcaagggc tggcgttgga aagcgaattt ctgccggaca
    12721 gcccgaacca ccctgaatgg ccgcaaccgg actgcttcct gcgtcctggc gaagagtatt
    12781 ccagcctgac ggaatatcag tttattgctg agtaatgtta agccctcgtc atgagggctt
    12841 tatcttcata ttgttcaaat caccagcaaa caccgacata tttgcaactc aatattcaca
    12901 acaaccttac actgcgccac tattttcgct atggttatgc gtaagcattg ctgttgcttc
    12961 gtcacggcaa tataatgaga attattatca ttaaaagatg atttgaggag taagtatatg
    13021 gctgtaacta agctggttct ggttcgtcat ggcgaaagtc agtggaacaa agaaaaccgt
    13081 ttcaccggtt ggtacgacgt ggatctgtct gagaaaggcg taagcgaagc aaaagcagca
    13141 ggtaagctgc tgaaagagga aggttacagc tttgactttg cttacacttc tgtgctgaaa
    13201 cgcgctatcc ataccctgtg gaatgtgctg gacgaactgg atcaggcatg gctgcccgtt
    13261 gagaaatcct ggaaactgaa cgaacgtcac tacggtgcgt tgcagggtct gaacaaagcg
    13321 gaaactgctg aaaagtatgg cgacgagcag gtgaaacagt ggcgtcgtgg ttttgcagtg
    13381 actccgccgg aactgactaa agatgatgag cgttatccgg gtcacgatcc gcgttacgcg
    13441 aaactgagcg agaaagaatt gccgctgacg gaaagcctgg cgctgaccat tgaccgcgtg
    13501 atcccttact ggaatgaaac cattctgccg cgtatgaaga gcggtgagcg cgtaatcatc
    13561 gccgcacacg gtaactccct gcgtgcgctg gtgaaatatc ttgataacat gagcgaagaa
    13621 gagattcttg agctgaatat cccgactggc gtgccgctgg tgtatgagtt cgacgagaat
    13681 ttcaaaccgc tgaaacgcta ttatctgggt aatgctgacg agatcgcggc gaaagcagca
    13741 gccgttgcga accagggtaa agcgaagtaa acgtcattcg tttaaaaaat gagaaagccg
    13801 actgcaagtg agtcggcttt tttgtttgtc gttgctaaca atggagcaca ttgcctgatg
    13861 cgacgctgcg cgtcttatca ggcctgtggt gattcatcgg atacgccact ctgacggcgc
    13921 atccgataat taaaccttac ccgcgacgcg cttttactgc attcgccagt tgacgtaaca
    13981 gagcatcggt atcttcccag ccaatgcagg catcggtgat gctcttaccg taggccagcg
    14041 gctccccgct ctcgaggctc tgattgcctt ccaccagatg gctttccacc atcacgccaa
    14101 taatggcctt ttcgccaccg gcaatctgct ggcaaacatc agcacaaaca tccatctgct
    14161 ttttgaattg tttggacgag ttagcatggc tgaaatcgat catcacctgt gctggcaggc
    14221 ctgctttgtt cagcccttct ttcacttcag caacgtgctt cgcgctgtag ttaggctctt
    14281 taccgccgcg cagaatgata tggcaatcac cgttaccgct ggtattcaca atcgccgaat
    14341 gcccccattt cgttacggac aggaagcagt gcggcgcacc ggcggcatta atagcatcga
    14401 tagccacttt aatcgtaccg tcggtgccat ttttgaagcc gaccggacaa gaaagccctg
    14461 atgccagttc gcggtgcacc tgcgattcgg tggtacgtgc gccaattgcg ccccagctca
    14521 tcaggtcagc gagatattgt ggggtgatca tatcgagaaa ctcacccgcc gctggcagac
    14581 cgctgtcgtt aatatcaagc agcaatttac gggctatacg cagaccgtcg ttgatctgga
    14641 agctgttatc catatgcgga tcgttaatca gccctttcca gcccaccgtg gtacgcggct
    14701 tttcaaaata gacgcgcatt acgatttcca gctcatcttt cagctcttca cgcagcgcca
    14761 gcaagcgagt ggcatactct tttgctgcga caggatcatg aattgagcat gggccaatca
    14821 ccaccaacag gcgatcatca ttacctttca ggatcttatg gatcgctttt cgggcatggg
    14881 caaccgtatt cgcggcattt tcagtagcgg ggaatttttc cagcaatgcg acaggaggaa
    14941 gtaactcttt gatttctttg atgcgtaaat cgtcgttctg ataattcatg tctgttccag
    15001 tgttgccata cttatcttag tgaatgcaat acttccaatc tatatcttcc gtcagaatgt
    15061 gtaaacgggg ttttacacta tgaacggatt aatcctggaa ttagcaagaa aaacgccaga
    15121 ttgtcgcgaa aaacgagatc tctcctacaa tttctaactg taactccttt gtttgttaat
    15181 tatttcaaga ttctctgctg cgtttcataa cctggctgaa atcttaaacc aatgccttat
    15241 attcacctgc aaatgcactg ttggaagagg ttatccgaca taacgaccat aacaggagca
    15301 tcctatgaaa atgacaaaac tggccacatt atttctgact gccactctaa gccttgccag
    15361 cggtgccgca ctggctgccg atagcggagc gcaaactaat aacggccagg caaacgccgc
    15421 agctgatgcg ggccaggtag cccctgacgc ccgtgaaaat gtcgcgccaa ataacgtcga
    15481 caataacggg gtaaataccg gttctggtgg cacaatgctg cattcggatg gttcttcaat
    15541 gaacaatgac ggaatgacca aagatgaaga gcacaagaac accatgtgca aagatggtcg
    15601 ctgtccggat atcaataaaa aggtacaaac cggtgatggc atcaataatg atgtcgatac
    15661 caaaaccgac ggtaccacgc agtaaccctg caaaatagcc aataatggga gagcggagtc
    15721 tctccctttt aatttttcac ttctaaaagc agtatgatgt cataaagtta agtgagaata
    15781 attaagggat gactcgtcat ggcgcactca cactcacaca cgtcctcaca cctgccagaa
    15841 gataataatg ctcgtcgctt gttgtatgct ttcggcgtga ctgccgggtt tatgctggta
    15901 gaagtcgttg gtggttttct ttctggttct ctggcattgc tggccgatgc gggtcatatg
    15961 ttgaccgata ctgccgccct gcttttcgcc ctactcgccg tgcaattttc acgtcgtcct
    16021 cccaccattc gccacacttt cggttggcta agactgacca cgctcgcggc gtttgtgaat
    16081 gccatagccc tcgtggtaat taccattttg attgtctggg aggcgataga acgcttccgc
    16141 acgccgcgtc cggtcgaggg cggcatgatg atggcaattg ccgtggctgg gttgctggca
    16201 aatatacttt ctttctggtt acttcatcac ggcagcgaag agaaaaacct caacgtacga
    16261 gcagcggcac tgcatgtgct cggggacctg ctgggttcgg ttggggctat tattgccgcg
    16321 ttgattatta tctggactgg ctggacacct gctgacccca ttctctcgat actggtgtcg
    16381 cttctggttc tgcgtagcgc gtggcgactg ttgaaagata gcgtgaatga attacttgaa
    16441 ggtgcaccgg tatcgctgga tatcgctgaa ctgaagcgtc gtatgtgccg ggaaatcccg
    16501 gaagtccgca atgtgcacca tgtacatgta tggatggtag gcgagaagcc ggtgatgacg
    16561 ttgcatgtgc aggtgatccc gccacacgat cacgatgcct tgttggatca gatccaacac
    16621 tacctgatgg atcactatca gattgagcac gccaccattc agatggaata tcaaccttgt
    16681 catgggccag actgccacct gaatgaaggg gtgtctggcc attcacatca tcaccattaa
    16741 tgggacagcg cgcgtgagcc tctttcacgt gcgctgttga tccacatccg gctgccgttg
    16801 agcgcaataa aggtcaggat gatgtactcc agagacatgg cgtaaacgcc ctgaagtgca
    16861 aaaataacga cgctaatcac gttaataatc acccacaaca gccagttttc cacatactta
    16921 cgcgtcatca gaatcattgc cacgatagat aacaccatca tgcatgaatc ccagaacggg
    16981 aaagcgtccg gttgcagttc aggcatcaca acctgtaatc ctaatgcttg catgatcatg
    17041 actgccacgc gggtcaaaaa tgcaaacacc ggattgataa ataccgtcat cagaccaatc
    17101 gaaacaacgc aaaccgccaa ccagctgagt gccttcggca atggcaacca gcgaattttc
    17161 aactccgcct cgttctgact ggtttgtcgc gaccacgcat accaaccgta aatattcgcg
    17221 gcaaagaaaa acacctgtaa tagcaggctg gcatacagct gaatctgaaa gaaaataatg
    17281 ccaaacaagg tgacgttgat caggccaaag aagtagttgc tgatcttctc cagactggca
    17341 aggccaatac acagcaaccc ggcgatcgtg cctaccgctt cgatccatga gagatcataa
    17401 ccgcctgccc ctattggtat atgtaccagg atattctgca cactaaaaaa atccatcttt
    17461 cccccaaagc gtaacgttac gccttattat tcgttatcca cgtagtgtag ccgcaaaatc
    17521 cagcatacga ttgagcggca ccagcgccct ctctcgcagc ctttcatcaa catgaacctc
    17581 gtgattgctt ccttcctgtt ctaatgcctc tgcgatggac tgaaggccat tcatggccat
    17641 ccacggacaa tgcgcgcagc tgcggcaggt tgcaccctca cctgcggttg gtgcttccag
    17701 taactcttta tctggcactg cctgctgcat tttgtagaaa atgccccgat cggttgccac
    17761 gataagccgc tgatgtggca atgttttcgc agcagcgatc agttgactgg tggaaccgac
    17821 cgcatccgcc atatcgacaa tagcttgtgg tgattctgga tgcaccagta tggcagaatc
    17881 cgggtattct tcttgcaagc gggttaacgc ctgagtctta aattcatcat gcacaataca
    17941 ggcaccctgc cagcatagaa tgtctccacc cgtctgtttt tgcacgtaac gccccagatg
    18001 tttgtcgggt gcccagatga ttttttcacc caaactatca agatgatcaa taagttcgac
    18061 ggaaatgctt gaagttacca cccaatctgc gcgcgctttt accgcagcag aagtgttggc
    18121 gtagacgacg acagtacgat cgggatgggc atcgcaaaat gcgttaaatt cttcaacagg
    18181 gcagccgaga tccagtgaac attcagcctg aagtgtcggc atcagaattg ttttttccgg
    18241 actgagaatt ttggcggttt ctcccataaa tctcacccca gcgactaaca aagtagaagc
    18301 gggatgcttt gcaccgaagc gcgccatttc cagagaatca gaaatacagc caccggtttc
    18361 ttctgccagt tgttgaattt cgggatcggt atagtagtgg gcaaccatca ccgcattacg
    18421 ttcttttagc agacgtttta tcttctcgcg gtaatacact ttttcatcaa tgcttaacgg
    18481 cgtcggcttc ggggggaaag gataaatcgc cgtgtctgga tcaaacatta cgctcatctt
    18541 accatctcgt tttacaggct taacgttaaa accgacatta gcgtaatatt cgctgttatc
    18601 ggtgtaattt gttttatatg ctaaacaaga tagtcgaatg gtggggaaaa gtcactcgaa
    18661 ttacgcctaa ttccaccgac gtagagttgc ttgcacagta agctctgacg atgtcatcag
    18721 gtttacaaag tgaaattgag gatgcctgaa tagcaaaaaa gcgcctttag ggcgcttttt
    18781 tacattggtg ggtcgtgcag gatgactcgc ttcgctcgcc cttcgggccg tcgccgcgag
    18841 cggctccgtt gtttcgctgc gctcaactca aacctgctgc aggttcgaat ctttgagtat
    18901 cgcagatagc aaaaaagcgc ctttagggcg cttttttaca ttggtgggtc gtgcaggatt
    18961 cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta acgacccctt
    19021 gcgggattat gctgctttaa tcattcagga tggtgggtcg tgcaggattc gaacctgcga
    19081 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccatac gggtgttacc
    19141 tgaaagattt ctactcgggg ccaatcttta agattggtgt gtcgtgcagg atgactcgct
    19201 tcgcttgccc tacgggccgt tgctggcgca acgttatcct tcacgcttaa catctgagtt
    19261 tgatgttaaa attggtgggt cgtgcaggat tcgaacctgc gaccaattga ttaaaagtca
    19321 actgctctac caactgagct aacgacccga gtggtgggtg atgacgggat cgaaccgccg
    19381 accccctcct tgtaagggag gtgctctccc agctgagcta atcacccgat actgcgctgg
    19441 atactacaaa ctacttccac cgaacccacc gtaattagtg gtgggtcgtg caggatgact
    19501 cgcttcgctc gccctacggg ccgttgctga cgcaacgtta tcctacacgc ttaacatctg
    19561 tcttcgatgc taaaactggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    19621 gtcaactgct ctaccaactg agctaacgac ccggtggtgg gtgatgacgg gatcgaaccg
    19681 ccgaccccct ccttgtaagg gaggtgctct cccagctgag ctaatcaccc aatactacgc
    19741 tggatactac aactaactat gttagtggtg ggtcgtgcag gatgactcga cttcgtctcg
    19801 cccttcgggc cgttgctgat gcaacgttat ccttcacgtt ttactaccgc ttccacctta
    19861 gcgattggtg ggtcgtgcag gattcgaacc tgcgaccaat tgattaaaag tcaactgctc
    19921 taccaactga gctaacgacc cactttttcg ttgctttcgg tttgtttgat atcccgtggc
    19981 aacggcggca tatattactg atttcagact tgagcgcaac aaaaatttcg atgcagatca
    20041 ctcaactgct tatgattcgc acgacacgac cagaaataat gcgacttctg gtcgtgtgtt
    20101 atgcattaca tcgcgttcag acgtttttgt gcctgtttag cgccatcagt accagggtat
    20161 ttactgataa cctgctggta tacggctttc gcttttgcgg tgtcaccttt gtcctgcatg
    20221 atgacgccga ctttaaacat cgcatcagca gcctttggtg acttcggata gtttttcact
    20281 accgaagcaa aatagtacgc cgcatcatct tttttaccct tgttgtagtt taactgaccc
    20341 agccaataat tggcgtttgg caggtaagtt gaatcagggt aatttttgat gaaattctga
    20401 aatgccacca ttgcgtcatc ctggcgggat ttatcctgca ccagcgcaat agctgcattg
    20461 taatccgtgt ttgcatcacc gcttttcacc ggcgcgccag cattcgcagt accagcatca
    20521 gctgtcggcg tcgttgatgc cgccgcaccg ctttgatcgc tgctggttga ttgcgccgct
    20581 gcaccaccgc tgctgaggct gtcgatctgc aacaggatct gcttttgccg ctccacgacc
    20641 tgattcagtt gatactgatt ttcctgaatc tgaccacgca gggaatcaat atcggattga
    20701 ttatcagaaa gttgttgctg gagttgggtt aaaagctggc tgtgagcatt agaaatacgc
    20761 tcaagttgag tgacgcggtc ttcgaccgag cctgagccga cactactgat tggtgcctga
    20821 gcaaaagcgg cccagggggc cgctatacca accagtaacg acagactcaa tagttgatgt
    20881 ctgaagttac tgctcatgca attctcttag taaaccagta ccgcacgacg gtttttggag
    20941 tatgccgctt cgtcatgacc cagtactgca ggtttttctt taccgtaaga aacgatggag
    21001 atctggtctg cagaaacgcc tttaccctgc aggtacatct taacggcgtt cgcacgacgt
    21061 tcacccaggg agatgttgta ttccggagta ccacgttcgt ccgcgtgacc ttctacggtg
    21121 actttgtaag acgggttgct acgcaggaag tttgcatgtg catccagcat ttgagcgaag
    21181 tcagaacgga tatcgtactt gtccagatcg aagtaaacga tgttgttctg ctgcagctgt
    21241 tgcatttgca gacgagcctg ctcttcggaa gacatgttgc cgttgccgcc gttcgcatcc
    21301 ataccagtgc cggcacccag catgccttcg ctgccgtcat tgctggcgtt cttgttggaa
    21361 gaacatgccg caattgccat aacaggcaga gcaatcatca gccctttcag cactttgttc
    21421 agttgcattt caatgattcc tttactattc aattaattat tatcacagat acggcgacca
    21481 ggcagggaat ttgacctgtc catcagttgc cggaagacgc gctttgaaac gcccatctgt
    21541 agaaaccaaa ttcagcacgg atcccatccc ctgagaagag ctgtagatta ccatagtgcc
    21601 gttaggtgcc agacttggcg tttcatccag gaacgtggac gacagaactt gtacgcctcc
    21661 cgttgccaga tcttgtttgg caatgtgctg ctgcccacca ttggagctga ccattaccat
    21721 aaatttaccg tcgctgctga catccgcatc ctggttctgc gaaccttccc aggtaatacg
    21781 ttgtggcgca ccgccgttga tattcacttt ataaacctgc ggacgaccgg cctggtcaga
    21841 agtaaatgcc aggttctggc tatccgggaa ccaggtcggt tcggtattgt tactgcgacc
    21901 atcagtcacc tggcggatct gaccagaagc caaatccatt acgtacaggt tcagactacc
    21961 ggttttcgac aaggcgaatg ccagtttgct gccgtctggc gagaatgcag gtgcaccgtt
    22021 gtgacgcggg aatgaagcca cctgacgtac agcgccattt gccagcgtct gaataaccag
    22081 cgcggaacga ccgctttcga aggtcacata agccagttta gaaccgtctg gtgaccacgc
    22141 cggagacatc agcggctgcg gtgaacggtg aacgacaaac tggttgtaac cgtcatagtc
    22201 agatacgcgc agttcatacg ggaactgacc gccgttggtc tgaacaacgt aggcaatacg
    22261 ggtacggaac gcacctttaa tgccggtcag tttttcaaac acttcatcac tggcggtatg
    22321 accagcataa cgcagccact gcttgttcac tttgtacgag ttctgagcaa gtacagtacc
    22381 cggtgcgccg ccagtgtcaa caagttgata agcaacattg taagaaccat ccggattcgg
    22441 agtgacctga ccgacaacta cagcgtcaat gcccagtgcg gaccatgcag ctggttgtac
    22501 ttcctgctca ctacccggct gctgtggcag acgagcgcga tctaacggat taaatttacc
    22561 gctgttacgc aagtcagcag caacgatgcc gccaatatct tcaggtgccg caccaggccc
    22621 cgcccactgg aaaggaacaa caccaatagg acgaccggaa tctacaccgc tgtcgatcac
    22681 aatgcggact tcagcatgca gaactgatgc ccacagtatg agaaaaccaa atgctactcg
    22741 taatgcctgc ttcatcatat ctcccttatc tggaccgatg gcccacgata atttagcaga
    22801 atgttaacaa actaaaatac acaaaactac cagaaccccg tggcaaccgg tgcctgatgt
    22861 tgaccgtccg aacagtcaac atcgcgatta cggtttgaag tccaatggcg cgtttttgaa
    22921 cacttcatat actgcctggc ttggtggttt cgggatcttc gcaagtttag ctgctgccaa
    22981 cgcagcctga caaagtgcgg gatcgccacc ttcaggtttg atatccagta acataccatc
    23041 gggtgccagt tttatgcgca gcgtacaggt tttgcctgca taggacgatg cgtcatagaa
    23101 cttactttcg atagcagatt taatctgccc ggcatagtta ttgatatcgg cccctgatgc
    23161 gccattgttt ttagtattac cactcccggc aggcgaagca ttgttccctt tcgcccctcc
    23221 ccccgttttc ggtgcattct taccagagct tagctcaccg aaaatatcat ctgcctctgc
    23281 ggccgctttt gctgcagcgg ctttttctgc cgctgctttc ttatcagctg ctgccttttc
    23341 agcagccgct ttttctgctg cttttttgtc ggctgcagct ttctctgctg ccgctttctt
    23401 atcagctgca gccttttcag cagccgcttt cttctcagct tctgctttgg ctttttcagc
    23461 agcttcagtt gccgctttct ttcttgctgc agctgcttct gccgcttccg ctttcttctt
    23521 cagtgccgca gcggctgcct cggctttttt ctgcgcttcg gctgcggctt tggcggcttc
    23581 tgcttctgct tttttctttg cgtctgcagc cgctttcttc gctgcttctt ccgcagcttt
    23641 agcatctgct tcggccttcg ctttagcatc tgccgccgct ttcgctgccg cctcttcagc
    23701 ttgcttctgc tttaactcgg cctgttttgc ggcttcttca gcctgctttt tctgctcctg
    23761 agccgctaac cgctctttct caagttgctt caggcgttcc tgttcagccg cttgtttctc
    23821 acgcagttct tcagcagcct gctgttcctt catcttgcgc tgttcatcag aacgcttcgc
    23881 gcttgattcc tggctttgca tgcgtttgta ctgctcaact accgcacctg aatcaaccat
    23941 gacagcgtcg atggacgaac caccgccgcc tccggctgaa gcttctatat tctcatcgaa
    24001 cgaactccag atcagcgccg caaataagat gacatgcagc actgctgaaa taattatcgc
    24061 ccgcttgagc ttgtcgtttt gttcggttgc ctttgacact ctcggtttcc aaaaactgtt
    24121 cgcctgttac ccgctctctt tcaagcaagg gaaacgcaga tgtttagata ggctgcgtca
    24181 ttaaaccaac cgatttcaca cccgcactat gtaacaagtt cagtgcttta attatttcat
    24241 cgtaaggcac atcttttgcg ccaccgatca gaaagaccgt tttcgggttg gccttgaaac
    24301 ggctggacac ttccgccacc acctgctctg gtggtaaacg ctccaggcga tctttctcaa
    24361 ccaccacggt gtactgacca ataccagaca cttcaacaat cactggcgga ttatcgttac
    24421 tgctcaccgc ctgtgattca gtagcgtctg gcagatcgac ctccacgctc tgggtgatga
    24481 tgggcgctgt cgccatgaag atcagcaaca gcaccagcag tacgtccagc aacggtacaa
    24541 tgttgatttc ggacttaagt tcgcgacgac ctcgtccacg cgctctggcc atggcttacc
    24601 ccttgttgct ctcgctaacg gtaaacgcct ggcggtgcag aatcgcggta aactcttcca
    24661 taaagttgtc gtaattcagt tccagtttgt ttacgcgctg gttgaggcgg ttataagcca
    24721 ttaccgccgg aattgcggcg aacagaccaa ttgcagtcgc aatcaacgct tctgcgatac
    24781 cgggcgcaac catttgcagt gttgcttgtt ttaccgcccc gagggcgata aaggcgtgca
    24841 tgatccccca gaccgtacca aacagaccaa tatacgggct gatggagcca accgtaccga
    24901 ggaacggaat gtgcgtttcc agattttcaa gttcacggtt catggagata cgcatagcac
    24961 gcgacgcccc ttccactacg gcttccggcg catggctatt ggcacgatgc aggcgcacaa
    25021 actctttgaa cccgctgtaa aagatttgtt ccgaaccagt cagattatcc cgtttcccct
    25081 ggctctcttg atagaggcga gagagttcga ttccagacca gaatttatct tcaaacgctt
    25141 cggcttcgcg cgccgctgcg ttaagaatac gggtccgctg gataataatg gcccaagatg
    25201 cgattgaaaa accaatcaaa atcaacatga taagtttaac cagaaggcta gccttcagga
    25261 acaaatcaag gatattcatg tcagtcactg cttaaactcc gcgacaatag acttgggaag
    25321 cgcacgaggc ttcattttga gtgggtcaac gcaaacaacc agaacctctg cttcattcag
    25381 caaagtattc tcggcgttga caatacgttg cgtgaaaacc aaagaggtgc cacgcattga
    25441 tgttatttca gtctgtattt cgagcatatc gtcgagccgc gcaggtgcgt aatattccac
    25501 cgtcatttta cgtaccacaa aggcaacgcg ttcagccatc agcgcctgct gactgaagtg
    25561 atgatgacgc agcatctctg tgcgtgctct ttcataaaaa gcgacgtaac tggcgtggta
    25621 caccacacca ccggcatcgg tatcttcata gtagacgcga accggccatc gaaacagcgt
    25681 tgtattcact ttacatcccg gtaatgcaac aaaagttaga gcttttaaac gctgctacta
    25741 tacgcgcgcg attgaggttt gggaatgagg gcaagttaag ggagcgt
//
LOCUS       DAZKIV010000056        25564 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZKIV010000056 DAZKIV010000000
VERSION     DAZKIV010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25564
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.056"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            238..657
                     /locus_tag="ACHOT6_002274"
     CDS             238..657
                     /locus_tag="ACHOT6_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFP9947482.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            657..1925
                     /gene="umuC"
                     /locus_tag="ACHOT6_002275"
     CDS             657..1925
                     /gene="umuC"
                     /locus_tag="ACHOT6_002275"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFP9947483.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDEVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(1971..2501)
                     /gene="dsbB"
                     /locus_tag="ACHOT6_002276"
     CDS             complement(1971..2501)
                     /gene="dsbB"
                     /locus_tag="ACHOT6_002276"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFP9947484.1"
                     /translation="MLRFLNQYSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2647..4188)
                     /gene="nhaB"
                     /locus_tag="ACHOT6_002277"
     CDS             complement(2647..4188)
                     /gene="nhaB"
                     /locus_tag="ACHOT6_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFP9947485.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHIREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4409..5128
                     /gene="fadR"
                     /locus_tag="ACHOT6_002278"
     CDS             4409..5128
                     /gene="fadR"
                     /locus_tag="ACHOT6_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFP9947486.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5180..6712)
                     /locus_tag="ACHOT6_002279"
     CDS             complement(5180..6712)
                     /locus_tag="ACHOT6_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFP9947487.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7042..8340
                     /gene="dadA"
                     /locus_tag="ACHOT6_002280"
     CDS             7042..8340
                     /gene="dadA"
                     /locus_tag="ACHOT6_002280"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFP9947488.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8350..9420
                     /gene="dadX"
                     /locus_tag="ACHOT6_002281"
     CDS             8350..9420
                     /gene="dadX"
                     /locus_tag="ACHOT6_002281"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFP9947489.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9807..11543)
                     /locus_tag="ACHOT6_002282"
     CDS             complement(9807..11543)
                     /locus_tag="ACHOT6_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFP9947490.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTAQEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11638..12552)
                     /gene="ldcA"
                     /locus_tag="ACHOT6_002283"
     CDS             complement(11638..12552)
                     /gene="ldcA"
                     /locus_tag="ACHOT6_002283"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFP9947491.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQYDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTINGHPVLKM"
     gene            12652..13263
                     /gene="emtA"
                     /locus_tag="ACHOT6_002284"
     CDS             12652..13263
                     /gene="emtA"
                     /locus_tag="ACHOT6_002284"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFP9947492.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13265..13999)
                     /locus_tag="ACHOT6_002285"
     CDS             complement(13265..13999)
                     /locus_tag="ACHOT6_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFP9947493.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14200..14454
                     /locus_tag="ACHOT6_002286"
     CDS             14200..14454
                     /locus_tag="ACHOT6_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9947494.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            14632..15072
                     /gene="ycgY"
                     /locus_tag="ACHOT6_002287"
     CDS             14632..15072
                     /gene="ycgY"
                     /locus_tag="ACHOT6_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFP9947495.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15151..16848)
                     /gene="treA"
                     /locus_tag="ACHOT6_002288"
     CDS             complement(15151..16848)
                     /gene="treA"
                     /locus_tag="ACHOT6_002288"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFP9947496.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17168..18586)
                     /gene="dhaM"
                     /locus_tag="ACHOT6_002289"
     CDS             complement(17168..18586)
                     /gene="dhaM"
                     /locus_tag="ACHOT6_002289"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFP9947497.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(18597..19229)
                     /gene="dhaL"
                     /locus_tag="ACHOT6_002290"
     CDS             complement(18597..19229)
                     /gene="dhaL"
                     /locus_tag="ACHOT6_002290"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFP9947498.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVGEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19240..20310)
                     /gene="dhaK"
                     /locus_tag="ACHOT6_002291"
     CDS             complement(19240..20310)
                     /gene="dhaK"
                     /locus_tag="ACHOT6_002291"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFP9947499.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20538..22457
                     /gene="dhaR"
                     /locus_tag="ACHOT6_002292"
     CDS             20538..22457
                     /gene="dhaR"
                     /locus_tag="ACHOT6_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFP9947500.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22557..25424)
                     /locus_tag="ACHOT6_002293"
     CDS             complement(22557..25424)
                     /locus_tag="ACHOT6_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9947501.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSSIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTAAAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDEMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         6319 a         6360 c         6587 g         6298 t
ORIGIN      
        1 atcaaacaat gtttaatgtc attatggcga atgcttctat tctatttttt tgccgggtga
       61 tatttttcat ttctgctgga tgagcgtcgt cgccagaagg ccacgtgagc acaggataag
      121 agaacgaaaa atcagcagtc tatgcagcga caaatattga tagcctgaat cagtattgat
      181 ctgctggcaa gaacagacta ctgtatataa aaacagtata acttcaggca gattattatg
      241 ttgtttatca agcctgcgga tctacgcgaa attgtgactt ttccgctatt tagcgatctt
      301 gttcagtgtg gctttccttc accggcagca gattacgttg aacagcgcat cgatctgaat
      361 caactgttga tccagcatcc cagcgcgact tacttcgtca aagcaagtgg tgattctatg
      421 attgatggtg gaattagtga cggtgattta ctgattgtcg atagcgctat taccgccagc
      481 catggtgata ttgtcatcgc tgctgttgac ggcgagttta cggtgaaaaa attgcaacta
      541 cgcccgacgg tacagcttat tcccatgaac agtgcgtact cgcccattac catcagtagc
      601 gaagatacgc tggatgtctt tggtgtggtg atccacgtcg ttaaggcgat gcgctgatgt
      661 ttgctctctg tgatgtaaac gcgttttatg ccagctgtga gacggtgttt cgccctgatt
      721 tatggggtaa accggtggtt gtgctatcga ataatgacgg ttgcgttatc gcccgaaacg
      781 ctgaggcaaa ggcgctgggt gttaaaatgg gcgatccctg gttcaaacaa aaagatctgt
      841 ttcgtcgctg tggcgtggtt tgctttagca gcaattatga gctttacgca gacatgagca
      901 atcgggtgat gtcgacgctg gaagaactat cgccccgcgt cgagatttac agtattgatg
      961 aggcattctg cgatctgaca ggtgtgcgta attgtcgcga tctgactgat tttggcagag
     1021 aaattcgcgc aacggtgcta caacgtaccc atcttactgt tggtgtgggg atcgcccaga
     1081 ccaaaacgtt ggctaagctt gccaatcatg cggcaaaaaa atggcagcgg cagacgggtg
     1141 gggtggtgga tttatcaaat ctggaacgcc agcgtaaatt aatgtctgct ctccccgtgg
     1201 atgaagtctg ggggattgga cggcggatca gcaaaaaact ggacgcgatg gggatcaaaa
     1261 ccgttctcga tttggcggat acagatatcc ggtttatccg taaacatttt aatgtcgtgc
     1321 tcgaaagaac ggtgcgtgaa ctgcgcggcg aaccctgttt gcaactggaa gagtttgcac
     1381 cgacgaagca ggaaattatc tgttcccgct cgtttggtga acgcatcacg gattatccat
     1441 cgatgcggca ggccatttgt agttacgctg cccgggcggc ggaaaaactt cgcagcgagc
     1501 atcaatattg tcggtttatc tccacattta ttaagacgtc accatttgcg ctcaatgaac
     1561 cttattacgg caatagcgcg tcggtaaaac tgctgacgcc cactcaggac agcagggata
     1621 tcattaacgc tgctacgcga tctctggatg ccatctggca agcgggccat cgctaccaaa
     1681 aagcgggcgt gatgctgggg gatttcttca gtcagggagt cgcgcagctc aatttattcg
     1741 atgacaacgc accgcgcccc gggagtgagc aattgatgac ggtaatggat acactgaatg
     1801 ctaaagaggg cagaggaaca ctctattttg ccgggcaggg gatccagcaa caatggcaga
     1861 tgaagcgtgc catgctttca ccacgttata caacgcgaag ttctgattta ctgcgggtca
     1921 aataaatata gcggcaggaa aaaagcgctc ccgcaggagc gctgaaggga ttagcgaccg
     1981 aacaggtcac gttttttcgc tttaaacggc tgggaaatca ccaccagcac cgcgacaatc
     2041 aggtaagcga taaaaatacc gagcagccac tgcggcattt ccagacctaa aaaatcccac
     2101 tgacgctcgg cacaatcgcc agaggcgaca aacacttgcg gcacccattt atccagcggc
     2161 agccattccg ggaaacgaac cataaaatca caggtggcaa acggcgaagg atagagctga
     2221 agcatggtgt gctcgtaagt taactgcaca ccgcggaacg cactatacaa ccagataacc
     2281 atcgctacat aacgcagcgg agttttcggg gcgatcgcgc caatcagcgc agcacccaga
     2341 acgccgaata acgcacaacg ttcataaata cagagcacgc aaggtttcag taacatcaca
     2401 tgctggaacc acagcgccgt cagttccagt gccagagcag taaacgccat caacagccac
     2461 gcgccccggc cttgtgaata ttggttcaaa aatcgcaaca taatcatttc cctgcaatat
     2521 gcatagagtg cgcagtttaa accaattcat tcgctgcgcc accaggaggc agcaaaaata
     2581 ggaagcaatt ggacaattat cgggcgaaaa ggcaaaccgg gcatttatat gcccggtaag
     2641 ttgttatcaa agcgttgcta tccagcccat ttgcataaac cattcggtta caggggcaag
     2701 cgtaaactcg acgcagagca agccgacgag tgtcagtacg agggtgtaag gcagggccat
     2761 ccacaccatg cggccataag agaggcgaat caatggcgcg agtgcagagg tcagcaggaa
     2821 caggaacgca gcctgaccgt tcggcgtagc gacggagggc agattggtac cggtattaat
     2881 ggcgaccgcc agcagctcgt attgcttcaa cgtgatagcg ccactttcca ttgccgcttt
     2941 cgcttcgttg atataaatcg tccccacgaa gacgttatcc gaaatggatg acagcagacc
     3001 gttgaaaata tagaacagcg acagctgagc atgttccgat gcctgcaaca caaactgaat
     3061 aattggcgaa aacagttgtt ggtcgataat caccgcgact accgagaaaa acaccgtcaa
     3121 cagtgcggtg aatggcagag attcggtgaa ggctttaccg atagcatgtt catcggtgac
     3181 accggtcaat gatgtcgcca gaataatgac tgacaaacca atcaagccaa cttccgccag
     3241 atgcaacgcc agcgcagtca ccagccagac gccaataatc gcctggacaa tcagacggat
     3301 tttatcctga cgggtacgct ggtggcggct ttgatcgtca aactgttgca acacttcgcg
     3361 gactttctcc ggcagcgttt caccgtagcc aaaccaacgc agcttctcta ccagcaggca
     3421 ggttaacagg ccacaaatca gaaccggaac ggtcaccggc gacatgcgca ggaagaaatc
     3481 gccaaaatgc cagccagccg ctttagcgat gatcaggttc tgtggttcgc ccaccatggt
     3541 catcacgccg cctaatgcag tgccgacacc ggcatgcatc atcaggctac gcagaaagcc
     3601 acggaactgt tccagaacca ctttgtaatg cttgtcgata tgactatcgt cttgcaggtc
     3661 ggtatcttca gtacgggaag aggctacgcg gtgataaata ccataaaaac cgactgcaac
     3721 gctgatcacc accgccacga cggttaaggc atcgaggaac gcggagagga acgccgccgc
     3781 cacgcaaaaa gagagcgaca gcagcatttt ggagcgaatg cttaacagca aacgggtaaa
     3841 tatgaacagc aacagctgtt tcataaaata gatacccgcc accataaaca tcaataacag
     3901 caagacttca agatttgccg ccacctcttc acggatgtgt tccgcgctgg tcatgccgat
     3961 gaataccgct tcgatagcca acagaccacc ggggagtagc gggtagcatt tcagggccat
     4021 cgccagagtg aaaataaatt ccgctaccag caaccagccc gcgacgaaag ggctgatgag
     4081 gaaaattaac gggtttacga ttaagaaaat gatgagggcg agtttgtacc agtcggggga
     4141 ctggcccaaa aaattgcgcc atagcgcgcg gccccaggag atctccatga tggtttccct
     4201 taccttacaa ataatcaatg atgtttttat gtttaaacgc aaagcttaac ggtcaggcag
     4261 gagtgaggca agtcttgata gtcaagggga aagagatgcg gaaaatgaag ccttgatccc
     4321 tttttcttct ttttgtctgc tatcagcgta gttagccctc tggtatgatg agtccaactt
     4381 tgttttgctg tgttatggaa atctcactat ggtcattaag gcgcaaagcc cggcgggttt
     4441 cgcggaagag tacattattg aaagtatctg gaataaccgc ttccctcccg ggactatttt
     4501 gcccgcagaa cgtgaacttt cagaattaat tggcgtaacg cgtactacgt tacgtgaagt
     4561 gttacagcgt ctggcacgag atggctggtt gaccattcaa catggcaagc cgacgaaggt
     4621 gaataatttc tgggaaactt ccggtttaaa tatccttgaa acactggcgc gactggatca
     4681 cgaaagtgtg ccgcagctta ttgataattt gctgtcggtg cgtaccaata tttccactat
     4741 ttttattcgc accgcgtttc gtcagcatcc cgataaagcg caggaagtgc tggctaccgc
     4801 taatgaagtg gccgatcacg ccgatgcctt tgccgagctg gattacaaca tattccgcgg
     4861 cctggcgttt gcttccggca acccgattta cggtctgatt cttaacggga tgaaagggct
     4921 gtatacgcgt attggtcgtc actatttcgc caatccggaa gcgcgcagtc tggcgctggg
     4981 cttctaccac aaactgtcgg cgttgtgcag tgaaggcgcg cacgatcagg tgtacgaaac
     5041 agtgcgtcgc tatgggcatg agagtggcga gatttggcac cggatgcaga aaaatctgcc
     5101 gggtgattta gccattcagg ggcgataatc ccttccgttt aaagagcaaa cccctcaaac
     5161 gaggggtttt ttgttgtttt tacagatttc ccattcttgg cgggcaacgt tccagcaact
     5221 cgatgctgcc gtcttcgttt tgctgttcga gcatcacatc aaatccccac aggcgatgca
     5281 catgcttcag cacttctttg cgcccccgat ccagcggtgc gcgattatgt ggaatgtaac
     5341 gcagcgtcag cgaacggtcg ccgcgcaaat ccacgttcca gatctgaata ttcggctcca
     5401 gattacttaa gttatattgc gacgataacc ggttacggat ctcccgataa ccttcttcat
     5461 tatgaatagc ggaaatctcc agataattat gccgatcatc atccagcacg gtgaagaagc
     5521 ggaaatcacg catcactttc ggtgacagaa actggctgat aaaactctca tctttgaaat
     5581 cacgcatcgc gaaatgcagc gtttccagcc agtcggaacc ggcgatatcc gggaaccagt
     5641 atttgtcttc ttccgttggc gactgacaaa tccgtttaat atcctggaac atggcgaacc
     5701 cgagggcata cgggttgatg ccgctgtacc acgggctgtt ataggggggc tggaagacca
     5761 cattggtgtg gctgtgcaaa aactccagca taaaacgttc cgttactttc ccttcatcat
     5821 acagatggtt aaggatggta tagtgccaga aggtcgccca gccttcgttc atcacctgag
     5881 tctgtttttg cggataaaaa tactggctca ccttacgcac aatacgcagg atttcacgct
     5941 gccatgattc cagcagtggc gcatttttct ccataaaata gagcaggttt tcttgtggtt
     6001 cggacggata gcggcgcgct tcagcaaccg ttttctcttc ctcgcgcttc ggcagggtac
     6061 gccagagcat attgacctga ctttgtagat actcttcgcg acttttctgc cgggctttct
     6121 cttcttgcag cgagattttt tgcgggcgtt tgtaccggtc cacgccgtag ttcatcagcg
     6181 cgtggcacga gtccagaagc cgttctactt catcaacgcc ataacgttct tcgcactcgg
     6241 taatatattt acgggcaaaa atcagataat cgacaatcga actggcgtcg gtccaactgc
     6301 ggaataagta attgttttta aagaaagagt tatgcccata gcaggcgtgt gccatcacca
     6361 gcgcctgcat ggtaatggtg ttctcttcca tcaggtaagc gatacacggg ttagagttaa
     6421 tgacgatttc ataggccagt ccttgctgac cgtgcttata cagccgttca gtctcgataa
     6481 actttttacc gaatgaccag tgcggatagt taattggcat gccgacgctg gagtaggcat
     6541 ccatcatctg ttctgaggtt atcacttcaa tctggtgcgg gtaggtatcc agccggtaga
     6601 gtttcgccac ccggtctatc tctgccagat aaacatccag caggtcgaac gtccagtcgg
     6661 gtccatcgct caaacgtgtg gtgtccttat tcatagaatc gatcgtcgcc atacgcgcac
     6721 ctcattgttg tcggcgctct ctgtgtggag cacctcattt caagcataga acacctgtta
     6781 aaaaccgcgt cgccggagaa tttttttctt tgcgatttct tattatcaga gtgccactaa
     6841 tccgcttctg aacggaattt tatgctggat aaaaagggcg ttcagcagga gatactaaag
     6901 acgccatatt gccgcagagt cagggagatg tgagccagct caccataaaa aagccgtatg
     6961 ttgaataata ttttcaactg agttatcaag atgtaattag attattattc ttttactgta
     7021 tctaccgtta tcggagtggc tatgcgagtt gtcatactgg gaagtggtgt ggtaggcgtt
     7081 gccagcgcct ggtacttaaa tcaggcagga catgaggtca ccgtcattga tcgggagccg
     7141 ggggccgccc tggaaaccag cgccgcgaat gccgggcaaa tctcccccgg atatgctgca
     7201 ccgtgggcgg caccaggtgt gcctttaaaa gcgatcaaat ggatgttcca gcgccatgcg
     7261 ccgctggcgg ttcgtctcga cggtacgcag tttcagttga aatggatgtg gcaaatgtta
     7321 cgtaactgcg acaccagcca ctacatggaa aacaaagggc ggatggtgcg tctggcggaa
     7381 tacagccgtg attgcctgaa agcattgcgc gccgaaacca atattcagta tgaagggcgt
     7441 cagggtggga cgctgcaact gttccgtacc gaacaacagt atgaaaatgc gacccgcgat
     7501 atcgccgtgc tggaagatgc cggcgtaccg tatcagctgc tggaatccag ccgcctggcg
     7561 gaagtggagc ccgcgctggc agaagtagcg cacaaactga cgggtggtct gcaactgccc
     7621 aatgatgaaa ccggagactg ccagctgttt acccagaatc tggcgcggat ggcagagcag
     7681 gcgggggtta aattccgctt taatacgccc gttgaccaac tgctttgcga cggcgagcaa
     7741 atctacggcg tgaagtgtgg cgatgaagtg attaaggccg atgcgtatgt gatggcgttt
     7801 ggttcttact cgacggcgat gctcaaaggc attgttgata ttccggtgta tccgctgaaa
     7861 ggctactcgc tgactattcc aattgcgcag gaagatggtg cgccagtatc caccattctt
     7921 gatgaaacct acaaaatcgc cattacccgt ttcgataacc gcattcgtgt tggcggaatg
     7981 gcggaaattg ttggttttaa caccgagctg ttgcaaccgc gtcgtgaaac gctggaaatg
     8041 gtggttcgcg atctctatcc acgcggcggt catgtcgagc aggcgacttt ctggacgggt
     8101 ttgcgcccga tgacgccaga cggtacgccg gttgtcgggc gtacacgctt taaaaatctg
     8161 tggctgaata ccggtcacgg cacgctcggc tggacgatgg cttgcggttc cggtcagttg
     8221 ttaagcgatc tgctctctgg tcgcacgcca gcgatcccat atgaggatct aagcgtagcg
     8281 cgctacagcc gtggatttac gccatcacgt ccgggccatt tacatggcgc acacagctaa
     8341 ggaaacgaga tgacccgtcc gatacaggcc agcctcgatc tgcaggcatt aaaacagaat
     8401 ctgtccattg tccgccaggc cgcgccgcac gcgcgcgtct ggtcggtggt aaaagcgaac
     8461 gcttacgggc atggtattga gcgtatctgg agcgcgctcg gggccaccga tggctttgca
     8521 ttacttaacc tggaagaggc aataacgtta cgtgagcgcg gctggaaggg gccgatcctg
     8581 atgctggaag gatttttcca tgcacaggat ctggagattt atgaccagca ccgcctgacc
     8641 acctgcgtac acagcaactg gcagctcaaa gcactgcaaa atgcgcggct aaaagcaccg
     8701 ttggatattt atcttaaagt gaacagtggg atgaatcggt tgggcttcca gcccgatcgc
     8761 gtgcttaccg tctggcagca gttgcgggca atggcgaatg ttggcgagat gaccctgatg
     8821 tcgcattttg ccgaagcgga acatcctgat ggaatttcca gcgcgatggc gcgtattgag
     8881 caggcggcgg aagggctgga gtgtcggcgt tcgttgtcca attcggcggc gactctgtgg
     8941 cacccggaag cgcattttga ctgggttcgg cctggcatta ttttgtatgg cgcttcgccg
     9001 tccggtcagt ggcgtgatat cgccaatacc ggattacgtc cggtgatgac gctaagcagt
     9061 gagattattg gtgtccagac gctaaaagcg ggtgagcgtg tgggctacgg cggtcgctat
     9121 actgcgcgcg atgaacagcg aatcggcatt gtcgccgcag ggtacgccga cggttatccg
     9181 cgccacgcgc ctaccggtac ccctgtttta gtggacggcg tgcgcaccat gacggtgggg
     9241 accgtctcga tggatatgct agcggtcgat ttaacgcctt gcccgcaggc gggtattggt
     9301 acgccggttg agctgtgggg caaggagatc aaaattgatg atgtcgccgc cgctgccgga
     9361 acggtgggct atgagttgat gtgcgcgctg gcgctacgcg tcccggttgt gacggtgtaa
     9421 cttgttgtaa gccggatcgg aggcaacgtc ttctgggtgc aaaaaaaatc atccatccgg
     9481 ctggtcagca actgtagttg ttaatgtgac agagccattg cccatgatag tgtccattaa
     9541 aaggatggac actatttccc cggaacctga actcaccgca caggcgttct acataaaacg
     9601 cttacgcttc attgttgact cgaactcgac ttcagataaa tcacgcttta cccttgatgg
     9661 agcctgtaca tagatttgtg taattgcctg attttgatat gttcaattca acatcaaatg
     9721 aaggttaaat tatggacgac aaacaattgc aggctcaggc tgcgttcagc aaagcatcgc
     9781 aaccggcgat agatgcttca ttaaatttaa gattcagctt cctcttcagc cacccgtacg
     9841 ccaatcttca acacttcatt atcttctttc tcggccaccg tccagatcat cccggcaaac
     9901 tctacctggt caccgacaac cggtgccgcg cctaacaact gctggacaat ttcacccagc
     9961 gtttgctgct tatcacgata ttctcgcccg tcttccaggc catatatcag cgcaacatca
    10021 gcatatttgg cgctggcttc gagaatgaag tcaccaaaga agcgttgatc cagcgcgacc
    10081 ggcggcgact ggctgaacag tttaccgagc gccggaagat cgcgttcccg accaattaca
    10141 cacaacacat cgccttcgcg cagtcgggtg ctgccggtag gatgaagcaa ctggttatca
    10201 cgaaacagtg ccgcaatacg cgtctctttt ggcatatgca aatcgcgcag tgccgcgccc
    10261 acgcaccatt tatcggcact caattgataa acaaactgct cccacggatt ttccggatga
    10321 atatccaggc caacgcgtga caccggacgt cccactggcg gaaccaccac tttggctttt
    10381 ttagccgccc acgagagtga tgttccctgc aagagcagtg aaaccagaac cacaaagaag
    10441 gcgacattaa agaacagacg tgcattctcc agccccgcca tcatcgggaa cacggccagg
    10501 atgattggca ccgcgccgcg taatcctacc cagctgataa acacgcgctc acgcagattg
    10561 aaaccgcgga aggggagcaa tccagcaaat accgaaaggg gacgggcgaa gaatatcatc
    10621 catgccgaca aaatgagcgc cggaatggca ataggcagca gatcgcttgg gttaaccaat
    10681 agccccagca ccaggaacat ggcgatttgc gccagccagg cgaggccgtc gaaattttgc
    10741 aggatgccgt agcggttgcg aatcggccga ttacccagca gaaaaccgca cagataaaca
    10801 gccagaatac cgctgccttc ctgcgccgtg gttaaagcaa aaattaaaat accgccgctt
    10861 aatgccagca atggatataa tccggcaggc agggcgatgc gattaatcat ttgcagcagt
    10921 aaataaccac cgccaagtcc gatgacaatc ccgaggccaa attgctgcag aatatcgacc
    10981 acgaacatcc aactgacact gctttcatgt tgctggatca tcgcaatcag ggtaatcgtc
    11041 agaaagaccg ccattggatc attactgccg gattcaattt ccagcgtcga gccaacacgt
    11101 tcgttaagcc ccttaccacc cagcagagaa aagaccgctg cagcatcggt agagccgacg
    11161 atagcgccga ttaataagcc ttcaatcaaa tcaagattaa acagccacgc cgccatcatg
    11221 ccggttaaac cagaggtgat aagcacgccc agcgtcgcca gcgacagtgc cggtcctaac
    11281 gccacacgaa aggaactggc ctgagtgcgc atcccgccgt cgagcaaaat aatcgccagt
    11341 gccaggttac ttaccatgta ggcgaagggg taattatcaa acgggatacc gccgacgcca
    11401 tcgactcctg ccagcatgcc gatcgccaga aaaataacca gaataggaat gccaagacgg
    11461 gaagaaaatg aactaagtaa tatactgctg gtgacaagga tggatcctaa gatgaaaagg
    11521 ctaattattg ttgtggcatc caacggtcgg ttactcctga ttacgctgtc tcttatataa
    11581 accctaccat attagcggca gagacagcgt tttacttagc cctgaagcgt gattttttta
    11641 cattttaaga acaggatgac cgttaatcgt caactgagtg ccttcccggg tgttattcag
    11701 aatggcatgt gcgcccaacg gcagcgtaac cgtgcgttgt tcatgaccaa aatcgaggcc
    11761 ggtaatgagt ggaatcgaca ggcgggaacg caaaaacgcg tacactgact ccaggttgta
    11821 acctgcgtca taatcattgg gcgtgctgcc gctaaagcta ccgagaataa tcgccttctg
    11881 acgtggcaaa attcccgcat gatagagctg caacagcata cgttcgacgc ggaaagggtg
    11941 ctcgttaata tcttccagca ccagaatacc gttctcaatt tttggcatcc acggtgtacc
    12001 aatcagtgaa atcagcatcg caagattgcc tccccacaac gtgccttcgg tctgacatgt
    12061 cgggccttcc ccttgccatt caatggtgaa ggtttcattg cgtaacgcca gccagaaatg
    12121 gtgctcggta aaggcgttca gttcatccgc gccaaagttt gctaccagca tggggccgct
    12181 aaaggtgatg acattgcctt gcgccagaag accgcactga atggcggtaa aatcgctatg
    12241 cccgcatatg agcaacgggt catattgttg gcgagcaacc agtgcctgcc agtcaatatc
    12301 tgccagtaaa cgactggcac cgtaaccgcc gcgtacagcc agcacgatgg tgttgggggt
    12361 ggtcagtctg gcaagggaat tgagatcttc caggcgctcc gtttccgtac ccgcaaaacg
    12421 ctcacaacga cgggcaataa cctcgacgtt attgacctga tgcccctcgt cggtaaggcg
    12481 ctggatacca cgcagcgcgg cgtgctgttt aatgcagtaa cccgatgggg caattaaatg
    12541 aaacagagac atggcaattc cttgctgaca acagaaacga aatgtatatc atgccgctta
    12601 ggtgtgccgt tgtcacctca acggcgattc caggctataa ggatagaaga agtgaaattg
    12661 agatggtttg cctttttgat tgtgttatta gcgggttgtt catcaaagca tgactatacg
    12721 aacccgccgt ggaacgcgaa agttccggtg caacgtgcga tgcagtggat gccaataagc
    12781 cagaaagccg gtgcagcctg gggcgtcgat ccacaattga tcacggcgat tatcgctatc
    12841 gaatcgggtg gtaatccgaa cgcggtgagt aaatcgaatg ccattggttt gatgcagtta
    12901 aaagcttcaa cctccggacg tgatgtttat cgccgtatgg gctggagtgg tgagccgacg
    12961 accagcgagc tgaaaaatcc agagcgtaat atttcaatgg gggcggctta cctgaatatt
    13021 ctggaaaccg gcccgctggc aggcattgaa gatccgaagg tactgcaata tgcgctggtg
    13081 gtgtcatacg ctaacggggc aggtgcgctg ctacggactt tctcgtcaga tcggaaaaag
    13141 gcgatcagca aaatcaacga tttggatgct gacgagttcc tcgatcacgt agcgcgaaat
    13201 catcctgcgc cgcaggctcc gcgctatatc tacaaacttg agcaggcact ggacgcgatg
    13261 taaatcagtc gcgcactttg tccgcttttt ctcgggcttc tcgctcgaga gagaaaataa
    13321 tccgctgtaa ttgccgctcc accgtcgggc tgacgttaag aaaacggaag ctcagacggg
    13381 gagtggtgat ggtttcattc ttgccatcaa tcactttgcg ctcgctgatg gagattaact
    13441 gggcgtcaaa gtgaaaaaca ccccattgcc ccatgttgac ttcaatctga gcgaagcgca
    13501 tgccttcttg taattcggca ggctttgctg tttccagtaa tgcgcccatg ccgcctaacg
    13561 acaaatcata caggcggaaa cgtaacgtac tgttatccgc cagtttggtc tggcaaaaat
    13621 aaggcggatg gagtggggcg gagatgcgga aatatcggcg tcgttgtaca aaccataagg
    13681 tgggaggcgg tacggtgata aatgccggaa gctgcaagta ttcactctgc tgtagttgtt
    13741 caacagtaaa ctcgactttc gcaccctgag tttcggcggt aatggtaatg tgctgtgcct
    13801 ttagcacggc gatgttgtct tcggcttgac tgccgaaatc cagcaccagt ttatccgggg
    13861 ttattgccag tattttgctg atcagctgcc cgccattcca actgagacgc aaaggaattg
    13921 cggctttgtg caaatcgcgt aacacgccca ggacggctaa cggattttgt ttcaggaact
    13981 gctcatggta atgactcacg cggaaaaact cctgactgac aaactgtctt tggtttatcg
    14041 gtcacagtta acaaaactta atacaaatgt gtgaatattt tttacatgct gttcttaaat
    14101 cagccgacat acgcccagca tcgatcccgc ccttgcctat acttagagcg ttgacgtaag
    14161 catttcttgc gtccggttca tcgaaacgag ggcctgaaca tgggaattat tgcctggatt
    14221 atttttggcc tgatagccgg cattatcgcc aagctaatca tgccggggcg tgatggtggt
    14281 ggatttttcc tgacctgtat tctcgggata gtcggtgcgg tggtcggcgg ctggctggcg
    14341 accatgtttg gcattggcgg ctccatcagt ggttttaatc tgcacagctt cctggtggcg
    14401 gtagtgggcg ctatcctggt gctgggcgta ttccgcctcc tgcgaagaga ataagatttt
    14461 cataaggcgg atagcgatac agatgccgct atccgctttc acatcagaac gtatactcga
    14521 cacctgcttt acgggtggaa caaatcaata tagcacttag atgcccgata aagtcggttc
    14581 ccggtaacaa caacagaatc cttttttaat tattgtttcg ttgtttttgg tgtgattcag
    14641 aaaataaata ttttagacag tagccagtca ggcgttaaaa atggggcgga tagtgaggcg
    14701 gttttggctg cgcttattga acatttagaa ttgatcaatc cttctggcag attaagccaa
    14761 aatacccgtt cggcaatgct tcagttacgc gaagaggaat ggtctgaatt ctttttttgg
    14821 ttgctaaatt ctctggagtg tctggattat gtgattatta atttaactcc agaaagcaaa
    14881 aaaacgttga tgagtgaaca ccgtaacaat attcaggtag caattgatgc tttatatagc
    14941 caacgtcggc gtaaatcgcc gggtgatgaa agtgaaacac ttacccgtag aaatgacgct
    15001 atcttcggca accacgtatg gcagacattt gcgcaatact tcccgccagg acttgaaaaa
    15061 ccgtctgtgt agaaaaaacc atagatatgg tgttatctgc cgacaacgcc atccggagaa
    15121 gccgaatgac gttgtcggag taagcgctgg ttaaggtgtg ggttgtgcct ctttggttga
    15181 gggttgcgtc gttgctgact taacggtcgg acgcgtcgcc ggaacattgt cacacggttg
    15241 ctctttcggg cagatcaaat ccagcatttt cagcgtcacg ccattggtcc agccaaagcc
    15301 atcctgtaat ggatattcgc caccgccgcc ccccgttccg gtggtgctga catcatattt
    15361 ttccaccagc tttttctccc ggtcataggt gtgctgaaca ttggtcagga agtgccagct
    15421 aatgtccatc gccacctctt tttgcccgta gttttgtaat ccttctgtcg cgacccactg
    15481 taacggtgcc cagccatttg gcgcatccca ttgttgccca cttttcaccg acgtggtgtt
    15541 caggccgccg ggttgcagca gatgtgtttt cgtcgccgtc gccattttgt tggcgcgatc
    15601 tttcgctgcc gcattgacgt acagcgggaa cagggcggcc gcggttaact gattgcgcac
    15661 tttatgactt ttcaggtcgt aatcggcata ccagccttgt tgatcgttcc acatgtattt
    15721 ttcgatccct ttttgacggg catttgccag cgtttcgtac tggtttgcca tcgcgttatc
    15781 tccggcagct ttgctggcgc gggcgaggat tttttccatt ttaaacatca ggctgttcag
    15841 atcgaccggt acgatgctgg tggtgcgtaa ggtatttaac tgctgcgggt tgtccatcca
    15901 gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc aggtcgcggt aaatttcagt
    15961 ggcaggtcga ttcggattgc ttttggcggt ggcaatatct tccacccatg actctggtcg
    16021 tggcgtatcg cgatcgtccc agtagcggtt gagaagggta ccatcctgaa gttttacaac
    16081 gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg tccatccagt aagcatattc
    16141 tttttgcatt tgcggcaggt attgcttcaa cgcggcatcg ccttcatgct gcgccagtaa
    16201 ctctaccatc agggcaaaga agggcggttg cgagcggctt aaatagtaac tgcggttgcc
    16261 gttgggaata tgaccgtaag tgtctatttc atgagcaaaa ttggccacca tatccgcgac
    16321 tttatcccag tgaccgcttt cggcaagtcc taacatggtg aagtaactgt cccagtaata
    16381 tacctcgcga aaacgtccgc ccggcacgac ataaggttcc ggcagcggta acagagaatc
    16441 ccatttttcg gtgttttcgg tagaacgcgt taataccggc caaagtccgt caatatgttc
    16501 gcgcagtgac tgtccctctg gcggaacata tttctcgccc tctttcggca gggtgaaatt
    16561 gacgttaacg aaatggcgca gatcaaatcc gctctggttt tgctgcatcc gataatcagc
    16621 aaggatcatc agcggatcgc tgttcggcac ggcatcggca aaggtttttt ggtccggaaa
    16681 aagtttggcg ttttgcacat cattaaacag cggccctaat aaaatatcag gcggctgtgg
    16741 tgttaccggt gtttcttctg cctgcaccga tagcgcagcg aaacacaaaa agatgcaggc
    16801 tggaattaac gccatttttt gcgggcgaga aggtgcgggg gatttcatca atcattctcc
    16861 tttggcgaaa ccgaataaaa cggtattcaa gtatcagaaa accttagttc aggatcgcac
    16921 tagctgcatg atcgaaatct cattctgcga actgtcaggc attttagaaa agggaattaa
    16981 agggataaat aacggcgaaa tagagtgatc aaataacgtc tgtttattag ccagccagcg
    17041 acatttggtt cgcgattagc gaagaaaaat catcataaat tggtaaaaaa tataacaggt
    17101 agttaaaaaa catcagtgct gggtaaattg ccggatgaca tcagaacgat gccatccgaa
    17161 cggtggctta accctgacgg ttgaaacgtt gcgttttaac gtccagcgtt agcgtttctt
    17221 ctggttgtat cgcatacagt ttctcaccct gctggcaaat ccagccaatc cccagttcac
    17281 gggcgattag ggcgctgtgg gataccggac ttccggcgct aaggcagata ccttttacaa
    17341 ccgccggatc cagttgcagt actgtggaag gataaatgtt ctccgccagt agaatagttg
    17401 gcgagttaaa ctgcgggagt tcttctttcg tttgggtcag gtggaccagg gtgcgatgca
    17461 gaagatcgtc cacatcaata tagcgagctt gtagatattc atcatccagt tgctggtatt
    17521 gctggctaag ttctttaaga acttgctgcc aggcatattc tgccgtgcaa tgttcatgct
    17581 gaaggagttc gcttgccgcc gccagcagtt ccggatcatc taacagtgta tggtgaccag
    17641 aaaagattgc ggcaatatcg tcaagcccgc tggcttctgc tttcgctgtt aacgtcatca
    17701 gatctaataa cgtgaagtca atagcctggc gtaatcgatc ttgttcttct tccacggtca
    17761 gggttgattt tgcctgtacc gtacataaaa ctggttgata ataaaaggct ttacccgaaa
    17821 caggcggaac gggacgcaga gtaggtggag cgacttcctc cgtttcacca aagttatctt
    17881 cagccagctg acggaaagcg atcagtgcct cttcagcttc tggccctttc gcaatcaggc
    17941 gcagcgtatc gttatagcga acttgtagta acgcaatctg gttaatactc tctggtgtga
    18001 cgcatttgcc gtttttttcc agcaacatat cggcattaaa tgtcgataag gtataaacca
    18061 gccgggaggc cggacgtaca tgcaggccgt tacggttttt tatgaccacc gccagagaac
    18121 gggcttcttc atcgtacgca ggacatgtgt cagagatttc agtgtcggag gacggtaaac
    18181 ccagttgttc acgtttggct tccagcgcat gcatagcgtc aaagataact ttgtcgatat
    18241 ccgctcccga ggccgcgctg accgttgctg ccagtgtacc ttcgaccaac ggcgcagcac
    18301 acaaacgtac ttttgcggcg atcttgggag ccagcaattc cagcgcagtt tcagcactca
    18361 ataatgcgct acccatatcc atcatgacca gcacatggtc ggcatcagca acagattcga
    18421 tggcctccat cactttgacg gcatcggtac caataggatt ttgtggatcg tcaattcccg
    18481 cggcaatggc gattttacaa ctatcactca ttaacatctg acgggctaat tcaccgacac
    18541 cttctcccag tcggctgcta tgtgaaacta tgaccaggtt taccatcacc aattccttac
    18601 tcttttgcgg ctaacgccaa catttgcatc ataaacatca ccgaggtcgc gccgggatcc
    18661 tggtgaccaa tactgcgttc accgagataa ctggcgcggc ctttgcgggc ttgcatcgta
    18721 atcgtacttt gtgcagcgga ttcggcgatt ctactggcag cttcgagcgc caccggaaca
    18781 gagagatttt gctcgctgga ctgacgtaac gattccacca ccggcaccca cacatcacac
    18841 atggttttat cgccaggttc ggctttcccg cgactgatta cgccgtccgc gccatcgcgg
    18901 aacatctgat aaagctcttc cagtgtcagg ctttgccgtg cctgggtcgc ctgtgcggcg
    18961 cggataaaga aggtaccgaa cagcggacca ctggcaccac cgacgctgga aagcagcgtc
    19021 ataccggtat tcttgagaat gaaaccgata tctttatctg cgatagcagg gagtttttcc
    19081 cccactttgc taaagcctcg gttcatattt agcccgtggt cagcgtcgcc aatttcgcga
    19141 tccagtccgg taagatactc gctctcggtg ctgaaaatat cgccacaacg agtgagccag
    19201 ttaacaattt gagttctgct cagtgacatt gctttctcct tatttacccc agttaagggc
    19261 cggggtgtgg accggggcgt cccagagtgc cagcgtttcg tcatcaactt tcagtaaggt
    19321 gattgagaaa ccggtcatat ccagtgaggt gcagtacgcg ccaattaaat tacgttcgat
    19381 agtcaatccc gcttgctggc aacgtgtggt caggcggtta tagacgccgt acagctcaga
    19441 aagcggagtt gcgccaagat tgttaaccag cgcaatcacc cgatcgccag actggagcgg
    19501 ttgtttggtt tgttgttctt cctgccaact gccttgttga taatcccaga aacgcaaagt
    19561 gcgatggtat gagccatttt ccagcagggt gtcgaacatt tcatcgacgg tttgatcaag
    19621 ggaagagaag gagcggcggt caatacccgg ctcaccatga atgccgacgc caaactccat
    19681 ctcattatcc gccagggtaa aagaaggttt gcccgcggca ggaacggtac aggcaccgag
    19741 agcgatacct attgagtggc cttgattatt caacttacgc cccagttccg cacaggcgtc
    19801 cagtgagtcg ccacgctccg ccgctgcgcc tacgagtttt tcaattaata cggtgttggc
    19861 aacgccgcgt cgcccggcgg tataaagact gtcttttacc gcaacgtcgt catcaatgac
    19921 cacagtggtc acttttacgc cgctatcgtg cagtaactcg gtcgctgttt caaagttaag
    19981 aatatcgccg gtgtaatttt tgataatcaa cagtacacct tcgccgccat caatttgcat
    20041 ggagcattca aagattttat cgggcgtcgg tgaggtgaaa atttcgcccg gacaggcccc
    20101 cgaaagcatc ccctgaccga tataaccaca gtgcatcggc tcgtgtccgc tgccgccacc
    20161 cgacagcagg gcgacttttc ctgcaacagg ggcatcagct cgggtgacat acaccggatc
    20221 ctgatgcagt gtcagcgatg gatgcgcttt cgccagtcct gccagttgtt cgtccagtac
    20281 gtcttgcaca tcattgatca attttttcat tattttgctc cagcaattac ggtagggcat
    20341 ggatgatgtt caacgacacg gcgaccactg actgccgatg aatccattgt gcatcaggca
    20401 agggaaaaga aaattagccc aaattatgtt tcatagtgaa acatatgctt taatgaatgt
    20461 tccatattga aacttttacg tgtattaata cttaaaattg cgagccggaa caccttttgt
    20521 cataagggat gcgggatatg agtggcgctt ttaacaacga tggtcggggc atatctccct
    20581 taattgcaac ctcctgggag cgatgcaata agctgatgaa acgggagaca tggaacgtac
    20641 cacatcaggc ccagggcgtg acatttgctt ctatttatcg gcgtaagaaa gcgatgctga
    20701 cgctcgggca ggctgcgctg gaagatgcct gggaatatat ggcaccgcga gagtgtgcgc
    20761 tgtttatcct cgatgaaacc gcctgcattc tcagccgtaa tggcgatccg caaaccttgc
    20821 agcagctaag tgcactggga ttcaatgacg gcacgtattg cgccgaggga attattggta
    20881 cttgtgcgct atcgttagcg gctatctctg gtcaggccgt gaaaacgatg gccgatcaac
    20941 atttcaaaca ggcactctgg aactgggcct tttgtgcaac gccgttgttt gacagcaagg
    21001 gccgattgac gggaacaata gcgctggcgt gtccggttga gcaaactacc gcagctgatt
    21061 tgccgttgac gttggcaatc gcccgcgagg tcggaaattt actgctgacg gacagtttgc
    21121 tcgctgaaac taaccgtcat ttaaatcaac ttaatgccct gttagaaagt atggatgatg
    21181 gcgtgattag ctgggacgag cagggtaatt tgcaatttat taatgcccag gcggcgcggg
    21241 tcttgcgcct tgacgcgacg gcaagtcagg gacgggcaat cactgaactc ttaacgttac
    21301 ccgccgtatt gcaacaagca ataaaacagg cacatccgct caaacacgta gaagcaacct
    21361 ttgaaagcca gcaccagttt attgatgcgg tgataaccct taaaccgata atagaaacgc
    21421 agggaaccag ctttattttg ttgctccatc ctgtggaaca gatgcggcag ttgatgacca
    21481 gtcaattagg aaaagtcagc cataccttcg ctcatatgcc acaggacgat ccgcaaaccc
    21541 gccgcttgat tcattttggt cgccaggcgg cgcgcagtag ctttcctgtc ctgctttgtg
    21601 gagaagaggg cgtgggcaag gcactgctaa gtcaggcaat tcataatgaa agcgagcgtg
    21661 ctgcaggtcc ttatatcgcc gtcaattgtg agttatatgg tgatgcggca ctggcggaag
    21721 aatttattgg tggcgatcgc acggacaatg aaaatggccg tctgagtcgg ctggaactgg
    21781 cgcacggcgg cacgctgttt cttgaaaaga ttgaatatct ggcggtggag ttacagtctg
    21841 ctttgcttca ggttatcaag cagggggtta tcacgcgact ggatgcgcgg cgtttaatac
    21901 caattgatgt caaagtgatt gcaacaacga ccgcggacct cgcaatgctg gtggaacaaa
    21961 atcgttttag tcgccagctg tattacgcgc tgcatgcatt tgaaattacc atcccgcctc
    22021 tgcgtatgcg gcgtggcagc attccggcgc tggtgaataa caaattacgc agtcttgaaa
    22081 aacgcttctc tacgcggctg aaaattgatg acgatgccct cgctcgcctg gtttcttgtg
    22141 catggccagg caacgatttt gaactttaca gcgtcatcga gaatcttgct ctgagtagtg
    22201 ataatgggcg cattcgcgtc agtgatttgc cggaacatct gtttaccgag caggcaacag
    22261 atgatgtcag cgccacccgc ctttccacca gtctgtcatt tgcggaagtt gaaaaagagg
    22321 caattattaa cgcagcccag gtcacaggcg gtcgcattca ggaaatgtcg gctttacttg
    22381 gaatcggccg cactacgctg tggcggaaaa tgaagcaaca tggcattgat gcagggcagt
    22441 ttaagcgccg ggtatgaaag acagaaacga tttctaatac atcagagtga tctatatttc
    22501 attccggcgc acgctaacat ttttcagcat cgtttaaggg cttgtctatc acgcacttaa
    22561 aagctgaagc gatatcctcc gttgacttgc ttctgatcaa agaggttacc ctgcgtgtaa
    22621 tccgcttcga ggtagaatgt gtgctgtttg ttatactgtg cactgactcc cacgccgtta
    22681 ttccagccat tacctttgaa actgtacttc tcccgggagt tgttcaacag atattcggta
    22741 tcccctgaaa actcgcggat agcgccagtc ttcacataga cattcagctg gctgttgcct
    22801 gcggtgatgt cataccccag tatcatgctg gcacgcccca gcagcgattc gtagtgattc
    22861 agatgtatat tgaggccatt actcgccttc atagccatct cattctggtg gctgtatgta
    22921 agctgggttt gcggctctat atagaaccca taaccagtag gggacaggtt gaacctctgc
    22981 ccggcttcca gggagatgct cattccattc gcagtgccgt tggcgttaac gccgttgttc
    23041 tgactgtcca gtacgtggaa actatttttc tggcgcgatg cttttataac gagatcgctg
    23101 taaaaaccgt tttgtgccat gtaactggcg tacattccca tgtagtctga acgtgcggta
    23161 ccgtcgcctc cgctatagtc cggcgatgca tgtgttgagc caatatacag accgacatac
    23221 aacggcattt catcagagag acgtttatcc ccaccaaact ggataccgct gtaacccatg
    23281 tcaaagccgc tcagtttgcc actggcaaag gagtccaggc ttcccccata actgcgcaac
    23341 cagatattac cgtctttact ctgatttcgc aggtcaccca tccgttgcat cagcgtacgg
    23401 ttttcaacat agttcaataa gtaaccgaca ttgagataat tgccgccagc agctgcggtc
    23461 gtggtgggtt taggcgtggg agcgggttca ggcgtaggat cggggttgac tatgggaggc
    23521 tgagcgggag ctggtgtggg ttcaggatta ggagtcggtt ccggaactgt cccggaagcg
    23581 taaagctccc agttagtgcc atttttacgc acatcgtaca gatatccccc caactcaacc
    23641 tgagaagacg cgctgaacga ggccgcgccg tcagtggttt ttaccactgt cagaacttca
    23701 tttcccgttg tggcctcgct gccctggttg cggatagcca atacgtgatt accagcacta
    23761 ctcccgctga tattcaataa atccccttta ttattaacgc cattaccctc gccaacaaca
    23821 tcagcacgca taataaaggt actgttaccg ctcaggttct ctacgtttaa tgtggcaaat
    23881 gtgccggcag ttgacccgtg gctggcaaaa tcgacagttg aatggctcag cgccagcgtg
    23941 tcgagattag aattacttgt tacgttccag acgctgttat tcattgcaac gtccagtttt
    24001 ccgccattga cattatcgct gagggaggaa cctgtccaaa ccgacccagg gtgcatatcc
    24061 agattgatta gcccaccttt ggaaagaacg ctaccgttga taagcatacg acccgaggcg
    24121 ttgatgcggc tggcggcata accatcgtca tgttgcgttg caatggccat ctggtcgggt
    24181 gtactcatat caatgaccag atcgctcgtg aggtcgatct ggctgttggt catggcataa
    24241 attcccctgg ctcctgccgc gccggtgata gccaaactgt ctccggttat tctaccgccg
    24301 ctgagcgccc acaaacccag cgccagactg ccattacgat caactgtaat atcggtattt
    24361 tgcatgttga caacagccgt tgccgtctgg gccgaggcac cataagaacc gccggaaaag
    24421 atgctgtttc gttgcgctgc cgtgccagaa aaattgattg tcgcgtctga accactggtg
    24481 acgaggccac cgccctgcgc ggaagaaata tggctatcag caccgatagt ggttgtacca
    24541 ccacgaactt cgacgccatt ggccgcggct ccagttacat caacagtgag tgcattcgcg
    24601 ctaacctggc caaagctcca gaggccgtgt gcattatcgc cattggtttt aatggaactg
    24661 tttgttccga gatcgacaac agagtttttc tgtacgttta tccccatggc gctgtagccc
    24721 tgaacatcga ttgtcaggtc tgtagccgta aaacgcgcag caccattggc gttattgccg
    24781 ttgagaccac cgatataaac acctgtactt ccatcggtcg tgattttact tccgcttcca
    24841 agatcgacac tggtgccata gtcattgatg gttaggccta taccgttcga gttttcaatg
    24901 gtgaattgag tggctgttag tgttgagcta tgcccaataa tgatgccgtc atcgttactc
    24961 ttaatggtgc tgcctgtgcc taagtcagca tgggtatagt caccaattaa gttaatgccg
    25021 atggcagagg tttgaccaac aacatctact gtgagtcggt tagctgtcag ggagctgttt
    25081 ttgccctgaa taatgattcc ttttgcatat ccagaggcgt cgttgacatt gacggtgaca
    25141 tcattaccca ggttgatatt gccaggcgtg tttcctgctg gggtcattac accatagagg
    25201 tttcctgatg gatcggcggt atctcctgta attctgtcac catcattaat agtaatattg
    25261 gtgccatatt gacttcctgt atcagcagct atcagcaggg gggaaaagat aattgcattc
    25321 aaaccaatag cgccaaattt tatcagttga attgagggcg aacggatttt taattcagcg
    25381 agacgatctg ctttggtatt cccattgtgt tgtttgatgc ccataccact cctatatagt
    25441 acccagacta tgggtctatg gattttgtca ttttgtttaa atcaatgaat aatatcctct
    25501 tatcatcatg atatttgtat ggtgcaatca tgatgagctt ctcagagaat aattctctat
    25561 tacc
//
LOCUS       DAZKIV010000057        25325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZKIV010000057 DAZKIV010000000
VERSION     DAZKIV010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.057"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..354
                     /gene="ydjO"
                     /locus_tag="ACHOT6_002294"
                     /pseudo
     CDS             1..354
                     /gene="ydjO"
                     /locus_tag="ACHOT6_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="protein YdjO"
     gene            complement(358..1749)
                     /gene="tcyP"
                     /locus_tag="ACHOT6_002295"
     CDS             complement(358..1749)
                     /gene="tcyP"
                     /locus_tag="ACHOT6_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFP9947502.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1882..2472)
                     /gene="ydjM"
                     /locus_tag="ACHOT6_002296"
     CDS             complement(1882..2472)
                     /gene="ydjM"
                     /locus_tag="ACHOT6_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9947503.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLF
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2635..3303)
                     /gene="hxpB"
                     /locus_tag="ACHOT6_002297"
     CDS             complement(2635..3303)
                     /gene="hxpB"
                     /locus_tag="ACHOT6_002297"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFP9947504.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRHEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3450..3986
                     /gene="yniB"
                     /locus_tag="ACHOT6_002298"
     CDS             3450..3986
                     /gene="yniB"
                     /locus_tag="ACHOT6_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFP9947505.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4027..4887)
                     /gene="yniA"
                     /locus_tag="ACHOT6_002299"
     CDS             complement(4027..4887)
                     /gene="yniA"
                     /locus_tag="ACHOT6_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFP9947506.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAERDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNALSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4993..5283)
                     /gene="ghoS"
                     /locus_tag="ACHOT6_002300"
     CDS             complement(4993..5283)
                     /gene="ghoS"
                     /locus_tag="ACHOT6_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFP9947507.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5384..6313)
                     /gene="pfkB"
                     /locus_tag="ACHOT6_002301"
     CDS             complement(5384..6313)
                     /gene="pfkB"
                     /locus_tag="ACHOT6_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFP9947508.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMALKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            6600..7358
                     /gene="ydiY"
                     /locus_tag="ACHOT6_002302"
     CDS             6600..7358
                     /gene="ydiY"
                     /locus_tag="ACHOT6_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFP9947509.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEYF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(7411..7518)
                     /gene="yniD"
                     /locus_tag="ACHOT6_002303"
     CDS             complement(7411..7518)
                     /gene="yniD"
                     /locus_tag="ACHOT6_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFP9947510.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(7690..9559)
                     /gene="espL1"
                     /locus_tag="ACHOT6_002304"
                     /pseudo
     CDS             complement(7690..9559)
                     /gene="espL1"
                     /locus_tag="ACHOT6_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10083..12011
                     /gene="thrS"
                     /locus_tag="ACHOT6_002305"
     CDS             10083..12011
                     /gene="thrS"
                     /locus_tag="ACHOT6_002305"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFP9947511.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALGKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12015..12557
                     /gene="infC"
                     /locus_tag="ACHOT6_002306"
     CDS             12015..12557
                     /gene="infC"
                     /locus_tag="ACHOT6_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFP9947512.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            12654..12851
                     /gene="rpmI"
                     /locus_tag="ACHOT6_002307"
     CDS             12654..12851
                     /gene="rpmI"
                     /locus_tag="ACHOT6_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFP9947513.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            12904..13260
                     /gene="rplT"
                     /locus_tag="ACHOT6_002308"
     CDS             12904..13260
                     /gene="rplT"
                     /locus_tag="ACHOT6_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFP9947514.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13377..13501
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13383..13427
                     /gene="pheM"
                     /locus_tag="ACHOT6_002309"
     CDS             13383..13427
                     /gene="pheM"
                     /locus_tag="ACHOT6_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFP9947515.1"
                     /translation="MNAAIFRFFFYFST"
     gene            13710..14693
                     /gene="pheS"
                     /locus_tag="ACHOT6_002310"
     CDS             13710..14693
                     /gene="pheS"
                     /locus_tag="ACHOT6_002310"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFP9947516.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            14708..17095
                     /gene="pheT"
                     /locus_tag="ACHOT6_002311"
     CDS             14708..17095
                     /gene="pheT"
                     /locus_tag="ACHOT6_002311"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFP9947517.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17100..17399
                     /gene="ihfA"
                     /locus_tag="ACHOT6_002312"
     CDS             17100..17399
                     /gene="ihfA"
                     /locus_tag="ACHOT6_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFP9947518.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17500..18480
                     /gene="btuC"
                     /locus_tag="ACHOT6_002313"
     CDS             17500..18480
                     /gene="btuC"
                     /locus_tag="ACHOT6_002313"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFP9947519.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            18543..19094
                     /gene="btuE"
                     /locus_tag="ACHOT6_002314"
     CDS             18543..19094
                     /gene="btuE"
                     /locus_tag="ACHOT6_002314"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFP9947520.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19094..19843
                     /gene="btuD"
                     /locus_tag="ACHOT6_002315"
     CDS             19094..19843
                     /gene="btuD"
                     /locus_tag="ACHOT6_002315"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFP9947521.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            19921..20385
                     /gene="nlpC"
                     /locus_tag="ACHOT6_002316"
     CDS             19921..20385
                     /gene="nlpC"
                     /locus_tag="ACHOT6_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9947522.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            20632..21345
                     /gene="ydiV"
                     /locus_tag="ACHOT6_002317"
     CDS             20632..21345
                     /gene="ydiV"
                     /locus_tag="ACHOT6_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFP9947523.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21408..22844
                     /gene="selO"
                     /locus_tag="ACHOT6_002318"
     CDS             21408..22844
                     /gene="selO"
                     /locus_tag="ACHOT6_002318"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFP9947524.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVVRTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRRRLQQDEVSDIERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(22848..23039)
                     /gene="hemP"
                     /locus_tag="ACHOT6_002319"
     CDS             complement(22848..23039)
                     /gene="hemP"
                     /locus_tag="ACHOT6_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFP9947525.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23171..24217)
                     /gene="aroH"
                     /locus_tag="ACHOT6_002320"
     CDS             complement(23171..24217)
                     /gene="aroH"
                     /locus_tag="ACHOT6_002320"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFP9947526.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24374..25207)
                     /gene="ppsR"
                     /locus_tag="ACHOT6_002321"
     CDS             complement(24374..25207)
                     /gene="ppsR"
                     /locus_tag="ACHOT6_002321"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFP9947527.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
BASE COUNT         6484 a         5925 c         6462 g         6454 t
ORIGIN      
        1 ccgggagcat ttttgctgcg attcaaaaaa aatgagagtc tcctaaagtt tccttcgata
       61 atatcaatgc ttaatatcaa tcttcttttt aatccactaa atcttccggg aatgggaagt
      121 ggtgtgcttg aagatattat gagtattcca gatagttcac ttagaaaacg tctcgggtat
      181 gaggttttaa gcttctcact acaggcacat tcattgagcc aggaatgtat tgataagttg
      241 gacatttttt tcgctgacga tctgtttaaa tatgagtcag tatgcatcgc cgcgatggaa
      301 catttaaaaa gtaaagcaac ggcccctata caaaacggac cgttgccagc ataagaatta
      361 acggtgtgcc agttcggcgt cgtcttcgct atccagaatg gctttatcgg tttgcttcag
      421 ccactggctg gtcagcgtgc cagctgtcat cgagccacta acgtttagcg ccgtacggcc
      481 catgtcgata agcggttcaa cggagattaa cagcgccacc agggttactg gcaggcccat
      541 cgcaggcagc acaatcagtg cggcgaaagt tgcaccacca ccgacaccgg caacgcctgc
      601 ggaactaacg gtaacaatac cgaccagcgt cgcaatccac atcgggtcca gcgggttaat
      661 gccaaccgta ggcgcaacca tcaccgccag cattgccgga tacaaaccgg cgcagccgtt
      721 ctgaccaatg gttgcaccga aagaggcggc gaaactggcg atggattcag gaacgcccag
      781 acgacgcgtt tgtgcttcca cattcagtgg gatagacgca gcactggaac ggctggtaaa
      841 ggcaaacgtc agcacaggcc ataccttacg gaagtacttc agcggactca cgccattaat
      901 gcccagcaga atgccatgca ctgcaaacat aatcagcaga ccgaggtagg acgcgacaac
      961 gaaacttccc agtttgatga tgtcttgcaa gttagaacct gcaaccactt tggtcattag
     1021 agccagaacg ccgtaagggg tcaactgcat gacaaggcga accagtttca tcacccagct
     1081 ttgtagggta tcgatagcgg ctaagacgcg ttcacctttc ggcgcatcat ccttcagcag
     1141 tttcaatgca gctacgccga ggaatgcggc aaaaattacc acgctgataa ttgacgtcgg
     1201 attggctccg gtaagatcgg caaacgggtt tttcgggata aaggacaaga ccagctgcgg
     1261 aacgctcaga tcagagactt taccaacata gttactttca atggcgttca gacgtgcagt
     1321 ttctgcacca ccctgaacca gaccttcagc cgtcaaacca aacaggttgg tgaccagcac
     1381 accgaccagc gccgcaatca gcgtggtaaa caaaagcgta ccgatggtca gaaaactgat
     1441 tttgcctaac tgagatgcgt tatgcagacg ggcaaccgcg ctcagaatag aggcgaagac
     1501 taacggcata acgatcattt gcagcagttg aacatagccg ttaccaacga tgttaaacca
     1561 ctgtacagaa tctttaagta cctggctgtc agaaccataa atggtatgca gggcaaggcc
     1621 aaaaaccaca cccatcacca gacccaccag cacttttttc gccagactcc actgtttatg
     1681 gcgggtctga gccagcgcaa acagcagtac aacgaacacc acgatgttcg caattaatgg
     1741 aaagttcatc cccgttctcc tgatcttatt atttctatca ctgcgtagca gtcatagtgt
     1801 tggcgcaaga ttaacagaac tgtgagctgg ctcttatatt caaatggaat tgcttattcc
     1861 agaaaggtga tttttgtctt tttagtattc aacctgatgc ttaataagcc gatgaaactg
     1921 gatctgcaag gtattgatca tttgcgatga ccaacgaaca gcgctgttct cgggtaatga
     1981 atggggcatc cataccgacc agacaaataa tgccatgcag ataaaacgtt ccagttggtt
     2041 gcccttttgg ggaaccagga taggcaagcg gaaacgccag cggcatggcc agagcagggg
     2101 aacaccggcg ggtgtcagca tatcggcaag tatgtggctc aaataaccca gcaccattcc
     2161 ttgtagcgca tcagccggaa tgaaccagcc ttccggaacc ttaaggtaaa aggttgccag
     2221 cagcgcaaat accgccagca gactgtgggt aaaaccacga tgcccaaaag cgcgggcgat
     2281 cggttttgat atccatttta atcgctgccc aagaaacgac tttgggtgat cgatgtccgg
     2341 gaacaaacac gtcaggattg cggaagggac aatatgccac cagtcaccct gtgccagcac
     2401 gggcgtcagc tcggcatttt tggcaaatac cgcacaagca atagaaaaga gaaggtgacc
     2461 ttccgccgtc atgataaaac tcactaaact gtcgatacgt acagtatagg atttttatac
     2521 agtgcacgga agtggtgacg gtgtaactct ttctcttaaa ttgacgatta atccgtcagt
     2581 tgaatgcaaa ttcagataaa gaaccagggg cagcgtgatc actgcccctg gtcattaacc
     2641 gagaaggtct tttgcggtga gttctgtcag cgatgagagt ttgacgtttg ctaatacaaa
     2701 acgtggatca ttttgcgctt ctggcgcagg aacgacgatg gaacgcatgc gggctgcttt
     2761 agaagcgatc atgccattta ccgagtcttc cagcgccacg caggtcagtg ggtcaacgcc
     2821 cagttttgct gcgcagtcga gatatacttg cggatgcggc ttgctgtaag gcagtttttc
     2881 agccgaggcg agggcatcga aactgtcgcg taagtcaaac atggtcaaca ctttttccag
     2941 catatgtagt ggtgacgcgg aggccagtcc caccaataaa ccttgttctt tgcataacgc
     3001 aacggcttcg cgcacgcctg gtaataatgg acgtgtctct tcaaccagtg aaatggcacg
     3061 ggcaataacc cgttctacta cttcgtgacg gcttggccca ttccacggtt gccgggcgta
     3121 ccaaagatcg accaccatat cgatgcgtaa acctaaggtg tccggcagct cgttacgacg
     3181 ggagatatcc acccccaggc ttgccatcac atccagttcg gctcgatccc ataaaggttc
     3241 tgagtcgata agtaatccat ccatatcaaa aattgcagca agaatctgac gcggggttga
     3301 cattacaacc tctcctttag agcattatgt gtaaggtatt aagccgtgct tatgattaag
     3361 cgcctatcat atcgccttta aagaatgggc gaaaacagat atctacaggt aaactaagtg
     3421 aaacgacgcg tcttaatcaa ggagaactca tgacgtatca acaagctgga cgcattgctg
     3481 ttttgaaaag gattttgggt tgggtgattt ttatacctgc attgatctct acattgattt
     3541 ctttgctgaa atttatgaat acccgacagg aaaaccagga aggcattaat gcggtcatgc
     3601 tcgactttac tcatgtcatg atcgatatga tgcaggcgaa tacgcctttt ttgaatctgt
     3661 tctggtataa ctccccgacg cccaatttta atggtggcgt gaacgttatg ttctgggtga
     3721 ttttcatcct gatttttgtc ggactggcgt tgcaggattc cggtgcccgg atgagtcgcc
     3781 aggcgcgctt cttgcgggaa ggtgttgaag atcaactcat tctggaaaaa gccaaagggg
     3841 aagaggggct aacgcgtgaa caaattgagt cccgtattgt tgttccgcac catactattt
     3901 tcctgcagtt tttctcactg tatatcctgc cggttatttg tattgctgca ggttatgtgt
     3961 tcttttctct gcttgggttt atttaatcaa aaggccgcac agagcggcca ttatcttcaa
     4021 cccatatcat gctgctaata atctatccag tgactgctga gcaataacca aatgctgacc
     4081 gccaaataac cttgcacgat ttaacagcgt gtagagttgg taaaccggtt gacgttcaag
     4141 gaaatcggca ggtagcgggg atactgactg atagccgtca tagatttgtg gcggctgttc
     4201 agtatgcagc ggtaacatcg ccaggtcgca ctctcggtca ccccagtagc aggccgggtc
     4261 gaaaatgtac gggccatccg gacccagcgc acagttgccg gaccataaat cgccgtgcaa
     4321 cagagaaggc tgcggttgat gcgaggccag acgctgctga atatgctcga cgatggcgtc
     4381 gatattgccg aaagcgatcc ctttctctgc tgccagttcc aactgccagc caatccgttg
     4441 ttcagcaaaa aacgttgacc agcgacgttg ccaggtgttg ggctgtggag ttgtggagag
     4501 cgcgttatcg aaatcaaggc caaattgtgg ttggtcactc cattgatgta aacgcgcaat
     4561 ttgctgacca agaataaatg cgctatgcgc atccagcgga cgaggtggga gataatccat
     4621 caccagaaaa ctgtagtcac ggtcagcgcc aactgcccaa accttaggta cggtgacggt
     4681 tttactacgc gacagtaact ccagttggtc ggcttcggcg gtaaaaccgg gaagcagttc
     4741 cctttcatca catttgacga aaaagtcacg ttctgcatag cgcaaatgcc atgcggcatg
     4801 gacttctccg ccaggcagtt cattacgcag ttcgatttcg ccttcaccta actgctcgct
     4861 caaaagacga ctgattgcct gccacatgat gtctctccca tgttgtctgc cagatcataa
     4921 ggttagcgca agaatacggt gtaaaaacac gagctaacgc acaccacaga ctgattattg
     4981 cccaaaaacc atttattttc tgttgctatt ccattcctcc caggtcgtga tggtgatttc
     5041 aggatctttg ccggttgcac tttgggtaag ccccgaaacc agttctctaa tttcttcttc
     5101 actttgggta ctgataagtc caaaagtgtt agttcccagc tcatggatat ttccctcatc
     5161 atccgtcatg gtgagcaaaa aaccgtcgcg agtcaggtaa ttattcaact cgttaatttc
     5221 agtcaatgta tcctcatgca acatgacggt tatgacataa cggacaagat cgccactggc
     5281 catagttcac ccctttgtta tcacatgact tttgagcata gtcggagaaa cgcgttgccg
     5341 acaggttggt ggtgattccc ccaatgctgg gggaatgttt ttgttagcgg gaaaggtaag
     5401 cgtaaatttt ttgcgtatcg tcatgggagc acagacgtgt tccctgattg agtgtggctg
     5461 cactccccgc agctacgcca aaacggacca tctcttcaag agaggcattt tctgccagtt
     5521 tcagtgccat cgcgccgacc atgctgtcac cagcgccaac ggtactctgg cttttcaccg
     5581 gtggtggcac cacctgaatg cagttttcac tatcaacacc cagcgctcct tgtggaccaa
     5641 gggaaacgac aacccgtttg gccttgccgc tattaacgat ttcctgcgcg gctttgcgga
     5701 catcgtccgg ctgggtgagt tcgcgattca ccagcgcact gagttctttt tggttaggct
     5761 taaccaactc gatgttacca attgccagtg ctgcacttaa cgcttcgcca gaactgtcga
     5821 cgatgcagcg gatcccttgt ttttgcgcag cggaaatcag ttgggttaat ttttccagct
     5881 tcacacctgg cggcaggctt ccgcttatga ccaggatggc cccggattca atttccagaa
     5941 cttgctcttc aagctggcga aactcatctt catttaatgc cgcgcctggc ataacaaaac
     6001 gatactgctc accgcttgct tccacatgta catgtaaatt ctgccgggtc cagtctttgg
     6061 cttctacagt agcgacgggg acattttcat ccgccaacag tgaaaccagg tgttcgccgg
     6121 tcgcgccacc cgccgggaag atcgctgtgg cactgcctcc aagatgggca atggcgcggg
     6181 cgacgttgat gccgccgccc ccgggttcga acaccggtgc ggtacagcgc agttttcctt
     6241 cgggataaat ttgcggggta attgttgcgc tatcgagaga gggcgcaagt gtcaacgtat
     6301 agatacgtac catcatttcc tcctataggc tgatttcagt ctggcaccga atcagcggaa
     6361 agtgaagaaa ttaacaatat gatttattgg agctttattt aaaattttgc agataaatat
     6421 atataaataa aaatttgttt tcgccacatg cttattcaaa aaatgaaata agaatacatt
     6481 gctaagttat tcgcgccttt ttataattcg ttacctttcc agggacgcta atctttgatc
     6541 cttaagaaag tttccgggac cgcgatggtc tctttttttg ttgtacggac tcttgataaa
     6601 tgaagctttt gaagacagtt cctgccatcg ttatgttggc ggggggcatg tttgcgtcac
     6661 tgaatgcagc tgccgatgat tccgttttta ctgtcatgga cgatcctgcc agcgctaaaa
     6721 aacctttcga aggtaatctg aacgcaggat atcttgcaca atcaggcaat acgaaaagct
     6781 cctcacttac ggctgacacc accatgacct ggtatggaca aaccactgcc tggtcgctgt
     6841 gggggaacgc cagtaatact tcttctaatg atgagcgttc ttcagaaaaa tatgcggcgg
     6901 gcgggcgtag ccgttttaat ttaactgatt atgactattt atttggtcag gcaagctggc
     6961 tgacagaccg ttataacggc tatcgagagc gcgatgtgtt gaccgcgggt tatggtcgcc
     7021 agtttttgaa tggcccggta cacagtttcc gctttgaatt cggcccaggt gtgcgttacg
     7081 acaaatatac cgacaatgcc agtgaaaccc agccgctggg ctatgcctcc ggggcttatg
     7141 catggcagtt gactgacaac gctaaattta cgcagggtgt ttcggtcttt ggcgcggaag
     7201 atacaacgct gaactccgaa agcgcattaa atgttgctat taatgaatat tttggactga
     7261 aggtggctta caacgtcaca tggaactctg aacctccaga gtctgcaccg gaacataccg
     7321 atcgtcggac aaccttatcg ctgggctaca gcatgtaaaa ttatctgccg gaccgtgtta
     7381 tgcggtctgg ctgtcttcgt ttgtcactgc tcagccccaa atcattgctg cccaacgtag
     7441 caacaacata gcgccacaga ttgccagtag caccaccacc atcttccagt gttttttatc
     7501 aaagcgttta gtcggcatac cagtgtcctg catatttctt gtgcgtgata acggagtgta
     7561 acaaaaggga cgggcatcaa gggatttttt gttattacag ccgttgggtg gcgttgtaca
     7621 cctggtcact gtttctgcgt tttatgaaaa aaataaaatt atgtatcgat atatatcgta
     7681 aatagctggt tataaatacg tctttatttc atcagcacta aaactcaggc tttggctgat
     7741 gacatttata gcagcataat atgtttctac agttttataa tgcttatgat gaatggctat
     7801 atgaacagct gacatgttgt catgattttt agcggccaat agtgtaaata actgatgttg
     7861 actaaaagaa tgtttttttg caaaagcacc cagcgtcgat atataagata acacgacgtc
     7921 ttcattaccc ttgctcatgg cgatgtataa tgcaggggtt ccctgtgcta gctttgctca
     7981 acttgtattt aaaggcgatg ccattaattt tagagaggaa ccgtgtgaca cacaaagggt
     8041 gattattttc cattgcggcg tataatcctg gctcaccatc atgtattttc atggctaaaa
     8101 tactcatcac cagttttttg tttaaaagct cttgtgctat tggagtggtt aatgcattca
     8161 gaaagatatc gattatttca tcgaatccat acaacatgga gatataaagt ccaggtgttt
     8221 cgttagcact tttagtttgc agtaatttaa ccagattatc ttcatgaatg atgtgattat
     8281 ctaccagtga ctgaatttct tgcataaata ttttcacgtt gtcagtatgt ccgttcatca
     8341 tagccaaaaa taaagcaggt gtaccaacgc tattctgcgc agttattatt tcaatgattt
     8401 ctttttcagg gagtatgggc aactgtttga ataatgtaat aagctgttga gtctggcctg
     8461 tagccattat taacaacatt gccgatggat gtataggggc aaatggcatt cgtgatgcaa
     8521 tacccgcgaa aatagcccct ggaactccca caggtaatag gggcagaata gcaattgcac
     8581 agttgcgaac atacctatca gcccaggtct gagcatcggg acctgaactg atgaacttat
     8641 caagcgaaag aaaggcccgt gctgtcctgt gggtgtctct gacggcaacg ttggtatcgt
     8701 tcgggtcgta aacggacaca acatagttta attgtccata cactgactgc tttattcgta
     8761 gccttactga caaagtatga ttgtctatat ataaaatcgc ggacatatga ctgacattgt
     8821 ttgccatcat tcgacaaaag aaaatatcta aagcgaagcc aaaatcctta ctcgcgataa
     8881 cgcgactccc tgcaggagga tggcgaagta atgctctgaa ctccgcgtcc cggactaagg
     8941 ggacatgctg ctgtatggat tcaacggagg agtaatgttt aatcaggttg actttgcctt
     9001 ttccatttct caatgaatca agggcgtatt gcaatgacag atggcgacaa aaaatagcgt
     9061 cagaaggaga ttgcaaaaaa catgcactac cattgagatt tactgttggg tgtatagttt
     9121 tttctctatg gattttttta gacagtttca tacaatggtt acgatcttca gcagttaact
     9181 gttcctgttc aatataatcc agtatgcttc gcaaatggtt agctttaata ggcgtggttt
     9241 tcagtaataa ggaaatgaaa tcttttttat agtgtaatgt aatgagtata ttttctgcat
     9301 ggatgggtga agtggcaaag ttaatggtaa aaagggcagt ggaataacaa ttttgtgata
     9361 tttttcgaca gtttgcggta aattcctgaa taacacagta tgacagcatc gaacttaagt
     9421 cagaggtatc aagtagggtg atgagctggc gcgcaaattt attaataggc tctgatgaag
     9481 gtgttggtat gtcgctatcg atgtgcaatc tatgaggaga cttataatga cttctgataa
     9541 tgatatcgtt ttgactcata atctattcgc cttatatata tctggaggtt ttgagaatga
     9601 tgattaatta ttatcgccag aacgaaatgt gcacgaaaaa ttgttataag aattgtgtca
     9661 cttttatcat tgtgatatga ccacttcgtc attaatctca tgagcgttta aaatactacc
     9721 gccaaatcac gcaacgaact ttgctggatt cagtttatgc tgctgtaaat ccgctcgcag
     9781 taaacctttc agacgcaccg tgatgttatc agtggttctt taagcgtttt gctggtgtac
     9841 tcactacaaa cgaattgcga atcgaatcaa tgtgaaacgg aaaggtacaa tctccctttt
     9901 tttagtgtta acatcgctca accgggtgga aggtgagagg ttaatgtctt tcatttgtgt
     9961 acctgattgc gaaccaattt agcatttgtt ggctagatgg tttcgcaatg aactgttaat
    10021 aaacaaattt ttctttgtat gtgatctttc gtgtgggtca ccactgcaaa taaggatata
    10081 aaatgcctgt tataactctt cctgatggca gccaacgcca ttacgaccac gctgtaagcc
    10141 ccatggatgt tgcgctggac attggtccag gtctggcgaa agcctgtatc gcagggcgcg
    10201 ttaatggcga actggttgat gcttgcgatc tgattgaaaa cgacgcacaa ctgtccatca
    10261 ttaccgccaa agacgaagac ggtctggaga tcattcgtca ctcctgtgcg cacctgttag
    10321 ggcacgcgat taaacaactt tggccgcata ccaaaatggc aatcggcccg gttattgaca
    10381 acggttttta ttacgacgtt gatcttgacc gcacgttaac ccaggaagat gtcgaagcac
    10441 tcgggaagcg gatgcatgag cttgctgaga aaaactacga cgtcattaag aagaaagtca
    10501 gctggcacga agcgcgtgaa actttcgcca accgtgggga gagctacaaa gtctccattc
    10561 ttgacgaaaa catcgcccat gatgacaagc caggtctgta cttccatgaa gaatatgtcg
    10621 atatgtgccg cggtccgcac gtaccgaaca tgcgtttctg ccatcatttc aaactaatga
    10681 aaacggcagg ggcttactgg cgtggcgaca gcaacaacaa aatgttgcaa cgtatttacg
    10741 gtacggcgtg ggcagacaaa aaagcgctta acgcttacct gcagcgcctg gaagaagccg
    10801 cgaaacgcga ccaccgtaaa atcggtaaac agctcgacct gtaccatatg caggaagaag
    10861 cgccgggtat ggtattctgg cacaacgacg gctggaccat cttccgtgaa ctggaagtgt
    10921 ttgttcgttc taaactgaaa gagtaccagt atcaggaagt taaaggtccg ttcatgatgg
    10981 accgtgtcct gtgggaaaaa accggtcact gggacaacta caaagatgca atgttcacca
    11041 cgtcttctga gaaccgtgaa tactgcatta agccgatgaa ctgcccgggt cacgtacaaa
    11101 ttttcaacca ggggctgaag tcttatcgcg atctgccgct gcgtatggcc gagtttggta
    11161 gctgccaccg taatgagccg tcaggttcgc tgcatggcct gatgcgcgtg cgtggattta
    11221 cccaggatga cgcgcatatc ttctgtactg aagaacaaat tcgcgatgaa gttaacggat
    11281 gtatccgttt agtctatgat atgtacagca cttttggctt cgagaagatc gtcgtcaaac
    11341 tctccactcg tcctgaaaaa cgtattggca gtgatgaaat gtgggatcgt gctgaggcgg
    11401 acctggcggt tgcgctggaa gaaaacaaca tcccgtttga atatcaactg ggtgaaggcg
    11461 ctttctacgg tccgaaaatt gaatttaccc tgtatgactg cctcgatcgt gcatggcagt
    11521 gcggtacagt acagctggac ttctctttgc cttctcgtct gagcgcttct tatgtaggcg
    11581 aagacaatga acgtaaagta ccggtaatga ttcaccgcgc aattctgggg tcgatggaac
    11641 gtttcatcgg gatcctgacc gaagaattcg ctggtttctt cccgacctgg ctcgcgccgg
    11701 ttcaggttgt tatcatgaat attaccgatt cgcagtctga atacgttaac gaattgacgc
    11761 aaaaactatc aaatgcgggc attcgtgtta aagcagactt gagaaatgag aagattggct
    11821 ttaaaatccg cgagcacact ttgcgtcgcg tcccatatat gctggtctgt ggtgataaag
    11881 aggtggaatc aggcaaagtt gccgttcgca cccgccgtgg taaagacctg ggaagcatgg
    11941 acgtaaatga agtgatcgag aagctgcaac aagagattcg cagccgcagt cttaaacaat
    12001 tggaggaata aggtattaaa ggcggaaaac gagttcaaac ggcgcgccct aaccgtatca
    12061 atggcgaaat tcgcgcccag gaagttcgct taacaggtct ggaaggcgag cagcttggta
    12121 ttgtgagtct gagagaagct ctggagaaag cagaagaagc cggagtagac ttagtcgaga
    12181 tcagccctaa cgccgagccg ccggtttgtc gtataatgga ttacggcaaa ttcctctatg
    12241 aaaagagcaa gtcttctaag gaacagaaga aaaagcaaaa agttatccag gttaaggaaa
    12301 ttaaattccg tcctggtaca gatgaaggcg actatcaggt aaaactccgc agcctgattc
    12361 gctttctcga agagggtgat aaagccaaaa tcacgctgcg tttccgcggt cgtgagatgg
    12421 cgcaccagca aatcggtatg gaagtgctta atcgcgtgaa agacgatttg caagaactgg
    12481 cagtggtcga atccttccca acgaagatcg aaggccgcca gatgatcatg gtgctcgctc
    12541 ctaagaagaa acagtaaggc cttcaagtag cactgtctgt ggagcctttg ggcttcacag
    12601 gcattgttcg cctttgtttc gtttattaac aatgcgaagt ggaagttatt aaaatgccaa
    12661 aaattaagac cgtacgcggt gctgctaagc gcttcaaaaa aaccggtaaa ggtggtttta
    12721 agcacaagca cgctaacctg cgtcacattc tgaccaaaaa agcgaccaaa cgtaaacgtc
    12781 acctgcgtcc gaaagccatg gtttccaaag gcgatctggg cttggtaatc gcgtgcctgc
    12841 cgtacgcata agccgttaac gtttttaact ttttaattag aatatagata caggagagca
    12901 catatggctc gcgtaaaacg tggtgttatt gcacgcgcac gtcacaagaa aattttgaaa
    12961 caagctaaag gctactacgg tgcgcgttct cgcgtatacc gcgttgcctt ccaggctgtt
    13021 atcaaagctg gtcagtatgc ttaccgtgac cgtcgtcaac gtaagcgtca gttccgtcaa
    13081 ctgtggattg cgcgtatcaa cgcagcagca cgtcagaacg gtatttctta cagcaaattc
    13141 atcaatggcc tgaaaaaagc ctctgttgaa atcgaccgta agatcctggc tgatatcgca
    13201 gtattcgaca aagtagcgtt caccgctctg gttgaaaaag cgaaagcagc tctggcataa
    13261 gccagttgaa agagggagct agtctccctc ttttcgtttc aacgccatca aaacattgac
    13321 ttttatcgcc gtagcctttt caataaaggt cttttgaaga gtaccaaaag gtaacgcaag
    13381 caatgaatgc tgctattttc cgcttctttt tttactttag cacctgaatc caggaggcta
    13441 gcgcgtgaga agagaaacgg aaaacagcgc ctgaaagcct cccagtggag gctttttttg
    13501 tatgcgcgtt ttgaaaaata aaaagccagt ctatcaggcg taattgtcgc agccagtctg
    13561 gacacggcca gcgcggagaa accggagcgt atttaagtac gtgagaattt cgagcactgc
    13621 ccgggaccaa aatggcaagt aaaatagcct gatgggatag gctctaagtc caacgaacca
    13681 gtgtcaccac tgacacaatg aggaaaacca tgtcacatct cgcagaactg gttgccagtg
    13741 cgaaggcggc cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat
    13801 atttgggtaa aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag
    13861 aagagcgtcc ggcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc
    13921 tgaatgcgcg taaagcggaa ctggaaagcg cagcgctgaa tgcgcgtctg gcggcggaaa
    13981 cgattgatgt ctccctgcca ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc
    14041 gtaccatcga ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc
    14101 cggaaatcga agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg
    14161 cgcgtgctga ccacgacact ttctggtttg acgctacccg cctgctgcgt acccagacct
    14221 ctggcgtaca gatccgcacc atgaaagccc agcagccacc gattcgtatc atcgcgcctg
    14281 gccgcgttta tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag
    14341 gtctgattgt tgataccaac atcagcttta ccaacctgaa aggcacgctg cacgacttcc
    14401 tgcgtaactt ctttgaggaa gatttgcaga ttcgcttccg tccttcctac ttcccgttta
    14461 ccgaaccttc tgcagaagtg gacgtcatgg gtaaaaacgg taaatggctg gaagtactgg
    14521 gctgcgggat ggtgcatccg aacgtgttgc gtaacgttgg catcgacccg gaagtttact
    14581 ctggtttcgc cttcgggatg gggatggagc gtctgactat gttgcgttac ggcgtcaccg
    14641 acctgcgttc attcttcgaa aacgatctgc gtttcctcaa acagtttaaa taaggcagga
    14701 atagattatg aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag
    14761 cgatacgctg gcgaatcaaa tcactatggc gggcctggaa gttgacggtg tagaaccggt
    14821 tgccggtagc ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa
    14881 cgctgacaaa ctgcgtgtga caaaagtgaa tgtcggcggc gatcgcctgc tggacatcgt
    14941 ctgcggtgcg ccaaactgcc gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt
    15001 tctgccgggt gatttcaaaa ttaaagcggc gaaactgcgt ggcgaaccgt ctgaagggat
    15061 gctgtgctcc ttttctgagc tgggaatttc tgacgatcac agtggcatta tcgaactgcc
    15121 tgcggatgcg ccgattggca ccgacatccg cgaatacctg aaactcgatg ataacaccat
    15181 cgaaatcagc gtgacaccaa accgtgccga ctgcttaggt atcattggtg ttgcgcgtga
    15241 cgttgccgtg ctgaatcagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac
    15301 catcgacgac acgttgccga ttacagtcga agcgccggaa gcctgcccgc gctatcttgg
    15361 ccgtgtggta aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact
    15421 gcgtcgttgc gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtactgct
    15481 cgaactgggc cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt
    15541 gcggatggca aaagagggcg aaacgctggt gctgcttgat ggcactgaag cgaagctgaa
    15601 tgctgacact ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg
    15661 tggcgaacac tctggcgtga atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt
    15721 cagcccgctg tctatcaccg gtcgtgctcg tcgtcatggc ctgcatactg atgcgtctca
    15781 ccgttatgag cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg
    15841 tctgctgatc gacatctgcg gtggtgaggc tggtccggta attgatatca ccaacgaagc
    15901 aacgctgccg aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg
    15961 ccatcatatt gctgatgaac aggtaactga cattctgcgt cgtctcggct gcgaagtgac
    16021 cgaaggcaaa gacgagtggc aggcagttgc gccgagctgg cgtttcgata tggagattga
    16081 agaagatctg gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc
    16141 ggtacaggca agcctgatta tgggtactca ccgtgaagct gacctgtcgc tcaagcgcgt
    16201 gaaaacgctg ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc
    16261 gaaagtgcag cagatgatcc atccaggcgt tgaagcctta ctgctgccaa gcccgatctc
    16321 tgttgaaatg tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta
    16381 caaccagaac cgtcagcaga accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc
    16441 agatactcag gcaccgttgg gcattcgtca ggatctgatg ttagccggtg tgatttgcgg
    16501 taaccgttac gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa
    16561 aggcgatctt gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc
    16621 agaagcgaat ccggcactgc atccggggca atccgcagcg atttatctga aaggggaacg
    16681 tattggtttt gttggagttg ttcatcctga actggaacgt aaactggatc ttaacggtcg
    16741 cactctggtg ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg
    16801 cgagatttct cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt
    16861 tcccgcagcg gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt
    16921 aaacttattt gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat
    16981 aagcctgatc ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt
    17041 cgccaaatgt gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta
    17101 tggcgcttac aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc
    17161 gggatgccaa agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg
    17221 gcgaacaggt gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc
    17281 cgggacgtaa cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct
    17341 tcagacccgg gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat
    17401 ctgatctaac taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc
    17461 gtaaaatcaa cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa
    17521 caacagcgac aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt
    17581 ctcttaagcc tttgcgccgg tgaacaatgg atctcgccag gtgactggtt tactcctcgt
    17641 ggcgaactgt tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt
    17701 gcggcgctgg ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa
    17761 cctggactac ttggcgtctc taacggcgca ggcgtggggc ttatcgccgc ggtattgctt
    17821 gggcaagggc aactccccaa ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc
    17881 atcactttaa tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg
    17941 gctggcgttg cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc
    18001 acctctgttg atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac
    18061 tggcggcaaa gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag
    18121 tccaggccga tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc
    18181 ctgtggttct ggcgcaatgt gctggtggcc gcgaccggct ggatggttgg cgtcagtgtg
    18241 gcgctggcgg gtgctatcgg ctttattggt ctggtgatcc cacatattct tcggttgtgt
    18301 ggtttaaccg atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg
    18361 ctgctggccg atattgtagc gcgcctggca ttagctgccg cagagctgcc tattggcgtg
    18421 gtcaccgcaa cgttgggtgc gccggtgttt atctggttat tgttaaaagc aagacgttag
    18481 ccgcaaaaag acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa
    18541 cgatgcaaga ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc
    18601 tggagaagta cgctggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa
    18661 cgccgcaata tgagcagttg gagaatattc agaaagcctg ggccgatcga ggttttgtgg
    18721 tgctgggatt cccgtgcaac cagtttctgg aacaagaacc gggcagcgat gaagagatta
    18781 aaacttactg taccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta
    18841 atggcgaagg acgccatccg ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg
    18901 cgccggaaga gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc
    18961 ctgatgatat tttatggaat tttgagaaat ttttagttgg cagggacggg ttggtcatcc
    19021 agcgtttttc cccggatatg acgcctgaag atcccattgt gatggaaagc attaaactgg
    19081 cgttggcaaa ataatgtcta ttgtgatgca gttacaggat gttgcggaaa ctacccgcct
    19141 ggggccgctt tctggcgagg ttcgggctgg ggagatcctg cacctggtgg ggccgaatgg
    19201 cgcgggtaag agtaccttgc ttgcacgaat ggccggaatg accagcggta agggaagcat
    19261 tcagttcgcg gggcaaccac tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc
    19321 ctacctttca caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact
    19381 gcatcagcac gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct
    19441 tgatgacaaa ctcggacgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg
    19501 tcttgctgcg gtggtgctgc aaatcacacc acaagccaat cccgcaggtc aattgctgct
    19561 tcttgatgag ccgatgaaca gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct
    19621 gagcgctctg tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac
    19681 attgcgtcat gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg
    19741 cagagaagag gtgctcacgc cgccaaatct ggcgcaggcc tatgggatga attttcgccg
    19801 tctggatatc gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga
    19861 aaatcgggta attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga
    19921 atgcgtttct gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa
    19981 gcaccgccgc caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac
    20041 cagctacaaa gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg
    20101 gactgttcgg gatttgtggt tgtaacgatg cgcgatcgtt tcgatttgca gctgccccga
    20161 gaaaccaaag aacaagcctc tatcggcacg caaattgata aagacgagtt gctgcctggt
    20221 gacctggtct ttttcaaaac gggttccgga caaaatggtt tgcatgtagg tatttacgat
    20281 accaacaacc aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcacttgat
    20341 aatgtctatt ggcagaaaaa tttctggcag gcgagacgaa tctagtatgt cgaaaaatgg
    20401 acggcgatga tacatcgccg tccatttact cctgaataca gtagtatttt gcagacaaaa
    20461 attccgtaaa tggattattc gtttataatc aggataatct aaaaaaagga cccctgaaaa
    20521 agtggttaac atggaacaaa tgaaagttac tccttctgta attgttatgt cttatggcct
    20581 catccctttt agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt
    20641 tttttggaga atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa
    20701 ctccttgtcg gcgttgagtt aattacccat ttctccagtg aagatggcac ggtgcgtatc
    20761 ccgacgagtc gggttatagc gcaacttacc gaggagcaac attggcaatt attttcagag
    20821 cagttggaat tactaaaatc atgccaacat ttttttattc aacataaact ctttgcctgg
    20881 cttaatttaa cgccacaagt ggctacatta ttattagaga gggataatta cgcaggcgaa
    20941 ctattaaaat atccgtttat tgaactgttg attaatgaga attacccgca tctcaatgaa
    21001 ggaaaagaca atcgcggtct tttatcctta tcgcaagtgt atccccttgt gttaggtaat
    21061 ttgggagcag gcaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg
    21121 ctggataaaa gttttattca gcaacaaatc acgcaccgct ccttcgaacc gtttattcgt
    21181 gcgatccagg cgcaaatttc cccttgttgc aattgcatca tcgcgggagg cattgatacg
    21241 gcagaaatat tggcacagat tacccccttc gatttccatg ctctccaggg atgcctgtgg
    21301 cctgccgttc ctataaatca gataacgacg ttggttcagc gataaccctc ctgtttgctg
    21361 gtgtttaaga cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt
    21421 ttattacccg ctggcgcgat gaattgccag aaacctatac agcactttcc cctacgcctt
    21481 taaataatgc ccggctgatt tggcataata ccgaactcgc taacacgctg agtattccat
    21541 cgtcgctgtt taaaaatgct gcaggtgtct ggggcggcga aaccctactg cctggtatgt
    21601 caccactggc gcaggtttac agtggtcatc agttcggcgt ctgggcgggc caactgggtg
    21661 atgggcgcgg cattttactt ggcgaacaac tgcttgctga tggcactaca atggactggc
    21721 atctgaaagg tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac
    21781 gttcgacgat acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga
    21841 cccgcgcgtt aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtagagccag
    21901 gtgcgatgct gatgcgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt
    21961 actatcgccg cgagccggaa aaggttcgtc agttagctga ctttgccatt cgtcattact
    22021 ggtcacatct tgaagatgat gaggacaaat accgtctctg gtttaacgat gttgtcgtac
    22081 gtaccgcatc gttaattgcc caatggcaga cggtcggctt tgctcatggg gtgatgaata
    22141 ccgacaacat gtcgctgctg gggctgacgc ttgattacgg gccgtttggt tttcttgatg
    22201 attacgaacc cggttttatt tgtaatcact cggatcatca agggcgttac agctttgata
    22261 atcaacctgc cgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg
    22321 ttgccgtaga tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt
    22381 atggacaacg gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc
    22441 tactgaatga attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc
    22501 gcatgctgag tctgaccgag cagcacagcg cggcgtcacc gctacgtgat gagtttattg
    22561 atcgtgcggc atttgatgac tggtttgccc gttatcggag gcgtttgcaa caagacgagg
    22621 ttagcgatat tgagcgtcag caactgatgc aaagcgttaa cccagctctg gtgttgcgca
    22681 actggttggc gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg gaattgcacc
    22741 gcctgcatga ggcgttgcga aatcctttca gcgacagagc tgatgactat gtcagtcgtc
    22801 cacctgactg gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttggtcaac
    22861 agcagcttgc cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc
    22921 agtttgccat ccggacctaa cagtgtctgg ctggtaattc gccggatggg ctgcggggaa
    22981 gcggtcttgt gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata
    23041 aaaattaata ataattgata taacaatgat tcttattatc aatattatgg caggccagag
    23101 caagcagtaa tgtgttgaga agacaacaag ttgcgcagaa atgcccatcc catcaggaat
    23161 gggcacgcat tcagaagcgg gtatctaccg cagaggcgag tttttcgacc aggcgttcgg
    23221 tatcttccca gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc
    23281 cgacgatttt ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg
    23341 tagagccatt gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct
    23401 gcttctggca gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt
    23461 gcagcgtatc gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac
    23521 gcataataat gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt
    23581 tgtctggcga gaggaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc
    23641 gcgtgttgcc atcggtacca tttttaaaac ctaccggaca ggagagtgct gaagccattt
    23701 cgcggtggat ctgactttcg gtagtacgag cgccaatcgc gccccaactg attaaatcag
    23761 caataaactg accggtcacc atatcgagga actcagtcgc ggtagggaca cccagctcat
    23821 ttacctgtaa aagtaatttg cgcgccagct ccagaccgtg attaacccga tagctgccgt
    23881 ttaaatccgg atcggagatt agccctttcc agccgacaac agttcgtggt ttttcaaaat
    23941 aggtgcgcat tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg
    24001 tggcgtactc cattgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca
    24061 gtcgcttatc ttcaccattc agtatttttt caattctgcg gcgggagtcg gtgacatggg
    24121 tggcgacgcc aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct
    24181 caatacgcgc agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc
    24241 ttctacggca ggactaccgg gaagtgatgc gcatcacgat aaaccaatcg cagctgattt
    24301 caagtgcgag gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aaataagcta
    24361 atgcactagt tctctagtac attcggcggc taagccccat gatatcgagg atcttggtgg
    24421 caatctcttc taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca
    24481 acgcttctac ttccgcgact tccatcctgc actgacgaag cgaggcatag cggctgttct
    24541 cccgacgttc ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt
    24601 tatgctgaag cggtttgagc gacgcgggta gcaccagatt gtccatatcg tcggcaataa
    24661 aggggtagtt tgccgcgcgg ataccaaact gcattgccag atacagactg gtgggggttt
    24721 taccacagcg agaaacaccg agcaggatca cctgagcctg gtccagattg cgcaacgaaa
    24781 tgccgtcatc gtgggcgagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga
    24841 gattattagg gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct
    24901 cttgttgtag cggggcaacc agcgcctgaa cgatatcctg gcaaaagcct tcactttgca
    24961 agatgatggc gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg
    25021 tctggtgata aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga
    25081 caaacggcag cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta
    25141 atacctccgc agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat
    25201 tatccatttt gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg
    25261 cacagaagcg tagaacgtta tgtctggttt ataaaatgaa ccttcaattt tattttttat
    25321 gaaaa
//
LOCUS       DAZKIV010000058        25178 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZKIV010000058 DAZKIV010000000
VERSION     DAZKIV010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25178
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.058"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..632)
                     /gene="fimB"
                     /locus_tag="ACHOT6_002322"
     CDS             complement(30..632)
                     /gene="fimB"
                     /locus_tag="ACHOT6_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFP9947528.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            2089..2805
                     /gene="nanC"
                     /locus_tag="ACHOT6_002323"
     CDS             2089..2805
                     /gene="nanC"
                     /locus_tag="ACHOT6_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFP9947529.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            2825..3931
                     /gene="nanM"
                     /locus_tag="ACHOT6_002324"
     CDS             2825..3931
                     /gene="nanM"
                     /locus_tag="ACHOT6_002324"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFP9947530.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTNIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGESAGGKAVTDSVLISVKDNKVTVQN"
     gene            3996..4976
                     /gene="nanS"
                     /locus_tag="ACHOT6_002325"
     CDS             3996..4976
                     /gene="nanS"
                     /locus_tag="ACHOT6_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9947531.1"
                     /translation="MDAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYHDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWGEK"
     gene            complement(4984..5088)
                     /locus_tag="ACHOT6_002326"
                     /pseudo
     CDS             complement(4984..5088)
                     /locus_tag="ACHOT6_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            5188..6060
                     /locus_tag="ACHOT6_002327"
     CDS             5188..6060
                     /locus_tag="ACHOT6_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000168579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015666 - restriction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorothioated DNA-binding restriction
                     endonuclease"
                     /protein_id="HFP9947532.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEEHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            6230..8305
                     /locus_tag="ACHOT6_002328"
     CDS             6230..8305
                     /locus_tag="ACHOT6_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9947533.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKSALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            8298..9641
                     /locus_tag="ACHOT6_002329"
     CDS             8298..9641
                     /locus_tag="ACHOT6_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HFP9947534.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAAIYSLVKHGVKDCFKLRPDLLIQSNKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     PQHFAFSETLKLWVVPFRIMAKRGERMMWPNDVSCTMSPQLDRMAASVSYSGGMR"
     gene            9638..10024
                     /locus_tag="ACHOT6_002330"
     CDS             9638..10024
                     /locus_tag="ACHOT6_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947535.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            10075..12222
                     /locus_tag="ACHOT6_002331"
     CDS             10075..12222
                     /locus_tag="ACHOT6_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HFP9947536.1"
                     /translation="MAKEAKKQDPQQQLEALFNKLVKQHSEFTQPLVELPEHEFAKAG
                     LIQGLVEGNLTPPVMLIALTEGHWEYAADTAKFDDLAYQLLSDIEGDWPVYAVVDDGL
                     NHRILALFGADGSDGTHGADTLPGLEELRSFDRMERDPTFRWSMRVYTRLMQRFDAFH
                     ENVYRVTKDKVNDKNDIIEEVAKLLFLETFRLHHDEDLTFKDDEGNTLRFRDVFNWQY
                     VESHGDKAVAQIKAAFEEFKSHENYVVISDDGSRNPIFSKETHLRLSVAKNYQDLLEA
                     IQNLGPVKTNDGKIAKEHGTLADVSGDLLGRVFDVFLRANFESKGGLGVYLTPNPVKQ
                     AMLEIAMHDIDDDDEMRSRLANGDFRFCDPTCGSFGFGSVALSQIDKWIDFKLVLADD
                     KKESLKQKLRDCAFTGADAAPRMVMLARVNMALQGAPKAQIFYTDNSLTTNALKPNSF
                     DLICTNPPFGTPKFDKGKNGQNSKANYEANMEQVLGGFRPTKKVVDSYNAWYDHVKMK
                     WEDIGDLELDENGEPKWAGYRTDLRRTGGTDKKPFYSLQPTTAGLALGSKPDSKGNWQ
                     PVGATIDPAVLFIDRCLQLLKPGGRLLIVLPDGILCNSGDRYVREYIMGKKDEKTGEF
                     VGGKAIVKAVISLPSDCFKLSGTGAKTSILYLQKRHANPNQPEQFLPEPQTDVFMAVA
                     ETLGYVVKNNIEDYNAGVANDLDKIVSAYKRGE"
     gene            12222..13748
                     /locus_tag="ACHOT6_002332"
     CDS             12222..13748
                     /locus_tag="ACHOT6_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFP9947537.1"
                     /translation="MDKFFRTNSSILVERLDPNFYQPSLVENDKLIKSKGWVELQSLY
                     EASGIGNTSAVERYYSEAADSIPYISGKVIKSFNIDLDECQRISLDSHKNELTKSALK
                     PTDVLVIRKGDMGNACVVPSEVNEANCSSEVIYLKMKASSDPYYLVSYLNCDQGQKAF
                     KRLGRGTIIPGVSLLDVPRLPIPKVSEFVQKYIGDKVRQAEQLRAWAKLLRTSVDAHL
                     NSLNLPINEPPALLNRVSAQTMEDRLDPRPYRTHYLCLVREIEKLPHDSISTLVELAS
                     GCPVSSNDFLENSGIPLVRIRNIGFDDFIGLDTGVSQDVYQDATKYQAKDKMIVVGMD
                     GIFRSQFFISDELPMLVNQRVAMLSPQNIRGELLTHWLNRPEGQMQLNQWAVKTTVEH
                     TSLSDIGRVLIPRLDKSLENKLADYLLNARLAYRYAKFLTQVAKTLVEALIEGQLTEQ
                     QLIQAQQALEDGDNSFDQAILSKLSAEGYAIEGATPLFSDVDELYSLLEEAAQAEAEE
                     "
     gene            13766..15052
                     /locus_tag="ACHOT6_002333"
     CDS             13766..15052
                     /locus_tag="ACHOT6_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947538.1"
                     /translation="MDSITTRQNNDPVNSEAALNLCLQLWHQGGLTASKAALLLAAVP
                     ALRSLLQPIIQPKKKDAETDIISAFSLTAPLLDAFSDLSQSGEWQLALLGLNPDVRQH
                     WINLAAARCQEAGAMSDPMVLVKLIQQLGNASEWVLAQLENADFSPQIIASPLAQTER
                     DLLGHSLNDNAAIPALCRILRTSHTLFTVSEQNEPPASIQAVDVTAKQLTNNWCSGRL
                     LALPNTLLDEHNLKPNADWLLVSRSGHDNMPLTELFAQQPWLFLLSLIIFVQDAWAAE
                     QRGGLLLTLPAGQNAFAPGQINLAVQGIEGDEVSLGSLAEFLVLLLGELNIPLYPALD
                     ANTESINRLNRVLSSFIAELLAKKIWQFTEAGRGEAGQYRIHTSFSDACYSLPLAPLF
                     GYKSQTLQRTIKQLAQNCYANKKRAANRINLQGSSL"
     gene            15049..19335
                     /locus_tag="ACHOT6_002334"
     CDS             15049..19335
                     /locus_tag="ACHOT6_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947539.1"
                     /translation="MSFELNEQMVDSLAKQHRWAATGVSDYPPRDPLVGQSRFFKRFQ
                     TFLHTVDHDDDRFAHVFAVEAEWGRGKSRLGHELVAQINDCSRGWFVRDEQGQLHDKQ
                     LFNRDTQDKYLALYIRYSQVASDYQNSDNWFAFGLYQALLPLATQTFDGSIQSEIAKQ
                     AYERLMPFGFKSELLADALQLNANHSEEDLYTDQTLVVSLVQAAYKVLKSFGVQYMLV
                     VLDELETVAEAATFGLEDEQDKRLDGQAIRLIGKAIKEEDPRRKLPWLRYVALCSPLL
                     GQQLREIQSVARRFELVELEHNAFADVSDYVASLLKDGKLSFNYPAGLVEAAYAMSGA
                     NFGWFNVVMANVDAVLSQYQQAGRSIKDVGDLFEAVLAGSGRVARHVLDKGAIEGIQT
                     RDQALLTECRSLLYGQLPVRLAEMGYATELLALKNEDLEPIAAHYRKLSIDRLQCRQA
                     LEQAKFRRDKDEWYYPAVDQALNLDTLLDNLRTFSIKEQAASGGKGAVLLPLSKGEFK
                     YLLSLLYDHPAIEFAADALWHKLVGETTELDASEATHIGPSVAMLLRLDLRYRSAQQN
                     SLIFKDPAMGDAHEQAMSELSKQSSQKPLIKALARVTGFVRLLDNNWTYDENLLSNKA
                     GSDDAPLAILTEPRGRQGGLLTLDGFKLHPEGKALFAWVSNLIELNNLHTFAANHSHQ
                     NGRTPVLALTASAHLMEQYSRLDERNELRDAILLHYVNPSEADQLERIGLSLAACQLH
                     GVNLTADSFTAKFKNKLHALTTFATDAIHKWRQRLQQRGLIAWPLKVDGKLSPNDRDL
                     LFKGWYQLAIAHPELNGILDLQQQHGVPVNELSSLLDKLKVPGSYIAKGYTADEHAGL
                     FTELNNVQRSQAQIPLFLARIAHPNKKHKWQFEGFKQQFYFAYVAETSVTAKGVFNDW
                     MWWCGELNLLTLTNPTEKQAIWEHYPRSRLENAIREAQNWFRGNDMGSYAANVEVMSR
                     VYGYARINEMFAPLGKNKLGFVTVEAKEQLEKAQSLFNVLKQQEEQLADMVEANDTKV
                     LAGLIHKRAEVLELVAKVKPLNSSRPMLKDAHILSLEDKTTSLYQRIEQACLFAEFVE
                     CSAERINNRLVDLIIDVETECAPLTNFPKRLYTNTLRTIGHILDGALKDDTSSATGRK
                     EQQADSDTLLHYLRKLDLGRAHDKLSALAQEVGLNLHNDQQLPIAEIQGHILSSYRNC
                     KERFSKLVNNLTEQKLRAQQLQEWLSSATAEYQFADDIAELPKLVMKLQLIEDAIADL
                     PSDAESKRQSMQNSLRNGQFSSLRDLPEELLKPARGQLTPIQGQLLKIEERLNQVRRN
                     SIAQVNSWLPLLKPLLASQKQAEPAALTLEDVSNLGVRELQQVCESTQAKWQSQGEQI
                     LNDTGLTLADWQPIYKALSQNQEPVLTPEQQKGLVDKGIVKMRLTFATGL"
     gene            19346..20194
                     /locus_tag="ACHOT6_002335"
     CDS             19346..20194
                     /locus_tag="ACHOT6_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947540.1"
                     /translation="MSRYLSAALASNRKGRFLQTVAGATPLMKDWISSPPASGLLIVQ
                     AEELTDANTMQHLYHWAMQAGCAALVINLKAEQFTLLAQLPYPLDWQLVPASLRGQEP
                     GLTALLASETDQAIAGFTGSADRYQHQAGDVVHTRYIRKHSNSGLLAFTTLPLWSLTL
                     LDHSELLVSWLNWFVDHAGIAERIIEPKAPSTDYTPDKHDLVVLLLLYAGGGMNLQAL
                     SEHNAVKLMFDVNSLDIVKRGEMLRQHDFIDDAGITATGKTCLQASQYWAYAPLLGEQ
                     LHTGTL"
     gene            20197..21354
                     /locus_tag="ACHOT6_002336"
     CDS             20197..21354
                     /locus_tag="ACHOT6_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001004905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947541.1"
                     /translation="MNENKTMNLAQSLAIDSQLRLPGSAGRLLKQVRDIEQVAPLFRD
                     AGLVKAVPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDS
                     VSELDDIDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLER
                     NVRHKQEYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTE
                     QGRKQILASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRI
                     EPNIEAKQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNA
                     MPLPQLLGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            21344..23029
                     /locus_tag="ACHOT6_002337"
     CDS             21344..23029
                     /locus_tag="ACHOT6_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HFP9947542.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTALETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRYADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            23037..23579
                     /locus_tag="ACHOT6_002338"
     CDS             23037..23579
                     /locus_tag="ACHOT6_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947543.1"
                     /translation="MPEVAWVNETFPLLASGKLLDQDVLVHSLGCSVWNTFGHEQSFM
                     AVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAKGQQKLEEKLKNLLEA
                     AQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGTQVIAAPDVEVVFSRF
                     LFIKNLNMIVLDRIHYEVLM"
     gene            23576..25045
                     /locus_tag="ACHOT6_002339"
     CDS             23576..25045
                     /locus_tag="ACHOT6_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFP9947544.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDIGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
BASE COUNT         7211 a         5564 c         5914 g         6489 t
ORIGIN      
        1 taacaccatc cctggtatct caactatctc tataaaacag cgtgacgctg tcgtcctctg
       61 gctctatccc agatgccgta aaaacgccct gcattgctgg cggtatacca gacagtatga
      121 cgaatattgc gatgcccaag ataatcctgg ataagtcgcg tatctattcc catattcgcc
      181 aaagcaaaac cacacgaatg gcgtaacatg tgcggatgaa tctccagtga caacccggca
      241 ttaccaccgg aagtcgagat aatatggtaa aactgttgcc gagaaagcgg atttccctta
      301 cgagataaaa atacccactc gctctcagca tgcgggtacg aagtacggat actcaaccag
      361 ttttttaaag cctgaacttc tttgttcaat agcggatgcg ttgttgaaaa gccttttttt
      421 aatcgatgga tatatataca ctttgcctta agatcaatat ccgaaatcct caatcgacaa
      481 atttcactcg cccggaaacc atgaataaaa caaagcaaag tcagacaata attacgtgct
      541 gcatgaggcc cggtatttgc tgctttaagg agtgattcga tttcactatg ggtcaggaag
      601 ttcctttttt tgttatcagc cttattcttc atcgtttttc ccttataatt acagacgcgc
      661 actagctgtg ctgggttagc aataatccaa catttttatc ctgattatgt ttataaaagc
      721 gaacgtttgc ttaataacta acgtaagtgg accagttctt ctgagtgaac ttaaatggag
      781 tagcaacagt taattataat aacgttgcca tacaaacgcc atgctcttgc atgctatgta
      841 cctttatata tttatcaatc ggatacgaat ctaataataa ccctcatcat taatgcaact
      901 aatcttatct atatatcatg tgatatgttc tgtaacaagt aaaatcaaca taaaaaattc
      961 agcaaaattt aatattcgtt caactagccg tctcacatat gacattatta gtcaactcca
     1021 tttgccacaa aatggctatt tcatggagcg cagtaatttc ttagtggcta ttatcatgct
     1081 aattcttagg tccctagcga ttattcctgg ctgattcgtc agcttacgcc atgagattgt
     1141 tacattcctt tttatcctgg agatatgggg gaacgcagga gatgattttt tcatctacat
     1201 tcgttggtaa atatagcttt ggtcactacg acttaaacct tcctcaatca cagccattcg
     1261 ccgggcgata cggcaaacaa actaagtgaa acatcatcct gaaaacacca acatcaacaa
     1321 gcctctccag atcgacttca gaagtgacca gttacaagcc acaaaccagg aaacatattt
     1381 catgatgaga attatgctca gcctgatggc gggtaaacac tgattttcac cacgctcact
     1441 gagttagctt gcaaatcttt ccttgtttat actttaaata aattacttat aaaacagata
     1501 attaaacaac aatctttggt ttgtgaaggc ctccgcaagt gttcgtctgg cttcacatgg
     1561 catcttcttc ttagaaaaat atcgacatat tttgtgacac gaattgcaaa tctggttttg
     1621 ttgtatggat tgcgtgattt ttgatctggt ataacaggta taaaggtaca ccaagatagt
     1681 caatgagaca gggcatctcg caatctatgg caaacatcac ttcagttctt tctcatcggg
     1741 tgatgaaaac gcacttcagt ctgaaaggaa tatgaaaatg agatcaacag acattctatt
     1801 ttatgactct gggtaaaatg gattgagtaa gtgatatagc ttacgaacat tcaaatcaat
     1861 taaacatcag aagagatttt atactcaggt atttaatctg gatctctgtt tatttaaata
     1921 atgtgaaaag agatttttca caggagacat tatacaaaaa aatataaaat acagctaccg
     1981 gttgccaaag acactataag cctggcaaaa aaatattaca caacataaat gctaattgtt
     2041 tatgcgggct ttgtattgct ttctgtatcc tacaaatgag tgaaatttat gaaaaaggct
     2101 aaaatacttt ctggcgtatt attactgtgc ttttcgtccc cattaatttc tcaggctgcg
     2161 acactggacg tacgtggtgg atatcgtagt ggaagccacg cctatgagac tcgactcaaa
     2221 gtcagtgagg gatggcaaaa tggatggtgg gcaagcatgg aaagtaatac ctggaatacc
     2281 attcatgata ataaaaagga aaatgccgca ctcaatgatg ttcaggttga agttaattac
     2341 gcgattaaac ttgatgatca atggacggtg cgcccgggaa tgttaacgca ttttagcagc
     2401 aacggcacac gctacggacc ctacgtaaaa ctgtcctggg acgcgacaaa agatcttaat
     2461 tttggcattc gctatcgtta cgactggaaa gcttaccgac aacaagactt atccggtgat
     2521 atgtctcgtg ataacgttca tcgttgggat ggatatgtca cttaccatat taatagtgat
     2581 ttcaccttcg catggcaaac gacgctatac agcaaacaga acgattatcg ctatgcaaac
     2641 cataagaaat gggcgacgga aaatgcattt gttctacaat accatatgac gcccgatatt
     2701 acgccataca tagaatatga ctacctggac cgtcagggtg tttacaacgg cagagataat
     2761 ttatcggaaa acagttatcg cattggtgtg tcatttaaac tgtagtagac aggagacagt
     2821 cacaatgaat aaaacaataa cggcgcttgc tatcatgatg gcttcatttg ccgcaaacgc
     2881 gtctgtatta cctgaaactc ctgtgccatt taaaagtggt accggagcaa ttgataacga
     2941 cactgtctac attggtttag gtagcgcagg tacggcatgg tacaagctgg atacacaggc
     3001 caaagataaa aaatggacag cgttagctgc attccctggt ggaccaagag atcaagcaac
     3061 ctcggcattt attgatggca atctgtatgt gtttggcggc attggcaaaa acagcgaggg
     3121 cttgactcag gtatttaatg acgtacacaa atacaacccc aaaaccaata gctgggttaa
     3181 attgatgtcg cacgcaccga tgggcatggc aggccatgtg acttttgtac acaacggcaa
     3241 ggcttatgtt actggcggtg ttaaccagaa tatcttcaat ggctattttg aagatctcaa
     3301 cgaggctgga aaagattcaa ccgctgtaga taaaatcaac gctcactatt ttgacaaaaa
     3361 agcagaagat tatttcttca ataagtttct gttgtctttt gatccctcaa cacagcaatg
     3421 gagttacgct ggcgaatctc cctggtacgg aacggctggt gcggcggttg tgaataaagg
     3481 tgataaaacc tggcttatta atggcgaagc caaaccagga ttgcgaacgg atgccgtatt
     3541 tgaacttgat ttcaccggta ataatttaaa atggaataag cttgctcccg tctcatcacc
     3601 agatggcgtc gctggcggtt ttgcggggat aagcaatgat tctcttatat ttgccggagg
     3661 ggccggattc aaaggttcac gagaaaatta ccagaacggt aagaactatg cgcatgaagg
     3721 cctgaaaaaa tcatatagca ctaatattca tctttggcat aacgggaaat gggataaatc
     3781 gggtgaatta tcgcaaggtc gggcctacgg agtatcattg ccctggaata atagtctatt
     3841 gattattggc ggtgaatctg caggcggcaa agcggtgacg gattcagttt taatctctgt
     3901 gaaggataat aaagtcacag tacaaaacta acgcttcagg gccccagtaa aggggccctg
     3961 ctatcaattt gtcatttcaa ttacgcgaaa attatatgga cgcaataata tcgccagatt
     4021 attactatgt tcttaccgtt gctggtcagt ctaatgccat ggcgtatggc gaaggactgc
     4081 cattaccaga cagggaagat gcgcctcatc ccagaattaa acaattagcg agatttgcgc
     4141 atacgcatcc cggaggcccg tcatgtcact ttaacgacat tattccactg actcactgcc
     4201 cacacgatgt tcaggatatg cagggttatc accatcctct ggcaacgaat catcaaacac
     4261 agtacggcac cgttggccag gcattgcata ttgcacggaa attactgccc tttattcctg
     4321 ataatgcagg ggttctcatc gttccatgtt gccgtggcgg atcggctttt accgcaggca
     4381 gcgaagggac atattcagaa cggcacggag ccagccatga tgcttgtcgt tggggaacgg
     4441 atactccgct gtatcatgat ttagtcagca gaacgcgagc cgcactggca aaaaatccgc
     4501 agaacaaatt cctcggcgta tgctggatgc aaggcgaatt tgacttaatg accagtgact
     4561 acgcgtcaca ccctcaacac tttaatcata tgattgaagc ctttcgtagg gatctaaaac
     4621 aataccattc tcagcttaat aatattactg acgcgccgtg gttttgcggc gataccacct
     4681 ggtactggaa agaaaatttc cctcatgcgt atgaagttat ttatggcaat tatcaaaata
     4741 atgttttagc caatattatt ttcgtcgact tccagcaaca aggtgaaaga ggactgacga
     4801 acgcgcctga tgaagacccg gacgatttaa gcacgggata ttacggttca gcgtaccggt
     4861 caccggagaa ctggacgacg gcactgcgaa gcagtcattt cagcgcggca gcccgtcggg
     4921 ggattatttc tgacaggttt gtagaagcaa ttttgcagtt ttggggcgaa aagtgagcgt
     4981 tcattaattt gatttattcg catccatcaa cgcttttaac tcctccggcg taaacggtgg
     5041 tttgcgtcgg tgcagcatcg catggcaatt agggtacaca ggcagcaaaa aaacaaatga
     5101 gatcaaagta atatatcttc acttgcaggc ttgaacagcc gaaaatttgc gagcatgatc
     5161 gcaattattt cactgccagg gatatcaatg gcttccagta aatcgctaca gcaggcaatt
     5221 gccaatataa aaatctggca caagggtgaa cagcgcgcgc cgcataagcc attgttattg
     5281 ctatacgtat tagcgggata cctgaatgga catccacgcc ttttcgatta tggatcggaa
     5341 atctacgaac ctctgcacag tttactggaa cgttttgggc cacagcgttc acagtaccga
     5401 cctgatatgc cgttctggcg attacaaggc gacggattct ggcaattaca caatgccgag
     5461 ctttgctcaa cagcagggag cagccgacaa ccgccagtga aggaactgaa cgaataccac
     5521 gttgcaggcg gtttcgatga acagcactac gccctggtaa ccggcaataa aaaactcatc
     5581 aatactctgg cgcaacagat actcgaagag cacttcaccg agagtattca ggaagaaatt
     5641 gctgacgagc tgggttttga tctccagcaa atccgtaaac agcgcgaccc gttatttcgc
     5701 aaaaatgtat tacgcgctta taactaccag tgcgcgatat gtggctttaa tatgcgccac
     5761 gacgacacca ccgtcgcgct ggaagcggcg catataaaat ggaaacagca cggcggtcct
     5821 tgtgaaattc ctaatggcct ggcgctttgc gcaattcacc ataaagcctt tgataaagga
     5881 tcgattggcc tggacgaaaa tatgcgggta ctggtatcag atgcggtaaa cggcggaggg
     5941 atcgttgaga gattattctg ggattttgac gggaaaacta ttgcgttgcc gcaggtgcgt
     6001 aaaaattacc cttatgaggt gtttgtagag tggcatcgga aagaggtttt tagggggtga
     6061 ggttgataac agcattcacc ctacaacctt acattgtatt taaattagag ctgagttaac
     6121 agatagttgt atatataata ggcacttgtc acaacgatta actcaatata gttagaatac
     6181 ctcatattaa ttaaaaaata agtaatgtca caacagagga aaagtcacga tgactcttgt
     6241 agatagtgtc gaagcaggta ggctaacgat tagtgaattg attgatgccc tggcgaaaga
     6301 taaaaattac acagcttcca gatggtatca gcgataccgt gcatttacga ctttgctaca
     6361 gcaaacctca acttttgctg agcctgcaac agatggtctg gtcaaacagc tttggtatga
     6421 gcgtgacaac ggcattgcaa gtattcgcca gggcgttcca tccttagcag aatatcagca
     6481 aagcctgcca ctgcttagag aactaactga acgaattcgg caacagccgg atgaagaaac
     6541 ttaccaatat gttggcaatg cacttcaaca agctaaagaa aacggacttc tcaagcgtat
     6601 gtattggagt ttgagaaatc gcgtctttgc cgcgttctcg ccagaaaact acaccagtac
     6661 tgtggatgag aatgctttta ataaagcagc agaattctta aatcagcact tccatctcgg
     6721 tttggtactg accggaaatt ggttacagaa aaactatgaa ttgaaacaag ccatacacgc
     6781 ccaatctcct gatacagatc cttattatgt gaatatggcc atctggcatc tctatgaatt
     6841 gctccgtgaa cgcgataatg aacaaaagca ggagaaagta gctagcacta catccataac
     6901 ccgcagtgag cccatcgaga acaagatcat cctacattca ccaactaacg tgatcttctt
     6961 tggcccccct ggcactggca agaccttcag gttgcagcaa aaaatgaaag agtacacttc
     7021 tcatgctgtt cccgctgatc gtgatgcctg gctggattct cgccttgaat cgttgaactg
     7081 gatgcaggtt ataacgctgg tgctgctcga tcttgggaaa cgagcgaaag ttcgccaaat
     7141 tattgaacat atgtggtttc aacgtaaggc attattaaac ggtcgtaatg gcaatctatc
     7201 gaatactgcc tgggcagctt tgcaatccta tacagttccc gagtcgttaa ccgttgatta
     7261 taagaatcgg cgtgagcctg ccgtatttaa caaaacagat aacagcgaat ggtttctagt
     7321 tgattcacag ctcgagcaag tggaggattt ggtagagctc tacgccgaac ttaaacgtgg
     7381 ccctaaatct gccgaagcca tccagcgttt tgcggtggtt acgttccacc aatcttacgg
     7441 ctatgaagaa tttattgaag gtatacgcgc tcgctctgac gagagtggca atatctctta
     7501 tcccattgag ccgggtatct ttatgcgcct ttgccaacgt gcgaatgccg atccaggaca
     7561 tcgctacgcc attttcattg atgagatcaa tcgcggtaac atatccaaga tctttggtga
     7621 actaatctca ctcattgaag tagacaagcg tgcaggcatg cccaatgcga tgagcctgca
     7681 actggcttat agcggtgatc acttcagcgt acccggcaat gtcgatatca tcggagccat
     7741 gaatacagcg gaccgttctt tagctctgat ggacacggct ttgcgccgtc gctttgactt
     7801 tgtcgaaatg atgcctgatc tctctttact gagtgaagct aaggtgaaag gcatagagct
     7861 cgagtcgttg ttagagaaac tcaatagccg catcgaggct ctttacgatc gtgaacatac
     7921 gctggggcat gcgttcttta tgccggtaaa aagtgcactc gatgccggtg atgaagaagc
     7981 tgcgtttaaa caattgaaga tcgcattcca gaaaaagatc attccgcttt tacaggaata
     8041 ctttttcgat gactggaaca agatccggtt ggtgctggca gacaatcaaa agcaagacga
     8101 caacctgcaa ttcgtgattg agaaaaccga cgatctcgat acgctttttg gtaacaacca
     8161 tggtttacga cgccatgatc agcaatcaac agcttatgag ctcaaagatt tcgatcaaga
     8221 gatctggaat attccacagg cttatcgttc aatttatcag ccccaacaga ctccccttga
     8281 tgagcaggca gtaaatcatg ggtgaagtta tctccgtttt tgaatatgac ctcttgggga
     8341 gtggaaaagc ggcttcagtt ggcgcaaaac cggtgcctcc acaggtcttt aattatttag
     8401 aagcgcttag cctggcaagt aatcaaggga gccagttcct caaactcacc tctcgctcag
     8461 gcttcaaatt gcttcaggtt caaaactacg cagggatgct ttcaacacct cacggtttcc
     8521 agcttgagat actgcccaag gttggtaaaa acctcacagc ggctaatgca cgtcaaacat
     8581 tgctgacgat gctaagccat ctgcctgggt tccgacatat tcaaacccag caagccactc
     8641 ttcaggctca gcgtatgcct ttgcttgaga tttttattaa ccagtttttg catagcgtca
     8701 gccaattgct taaacagggt ttacgctccg actatgtgag caagcaaggt aatctggctt
     8761 ttatgaaggg caagctgatg ctttctgcac agctgcgaca taacgcggtg aatcgccata
     8821 agttttgtgt cgattatgat gactacatgc ctgattgtgc agccaatcgg ctcttacact
     8881 ccgcactaga taagcttctt agtctgcagc tgtcatcaga taatcaacgc tggctttacg
     8941 aactgcgttt tgcgtttgac ggtattccac ttagccggga tattgaacgt gatataaaca
     9001 acttacgcct cgagcgtggt atggctcatt acaacgagcc aatggcatgg gcgcaattga
     9061 tcctgagggg aatgagcccg agtgctttgc agggaaacac caaagcgata tcacttttgt
     9121 tccctatgga agcggtattt gaatcctttg tggcacagac cctacccgac gagttaccac
     9181 ctcacctcaa agttttacct caagcagcaa tttattcttt ggtaaagcat ggagttaaag
     9241 attgctttaa gcttcgcccc gacttgctga ttcaatcaaa taaaccagtt caaaccgaaa
     9301 tggtgatgga tacaaaatgg aagctggtga atagcagcca gcagacaaaa tcactctatg
     9361 gactggcaca agctgacttc tatcaaatgt ttgcctacgg acaaaaatat cttggtggga
     9421 atggcgaaat gtatctgatt taccctgcgc atgatgactt tagccaaccg ataccgcagc
     9481 attttgcttt ctcggagact ttaaaattat gggttgtgcc attccggata atggcaaaac
     9541 gcggtgagag gatgatgtgg ccgaatgacg tgtcctgtac aatgagcccc caactggatc
     9601 ggatggctgc ttcagtatcc tatagtggag gcatgagatg acccatgaat accagcgaca
     9661 aagcctggaa caactaataa ctcagcaaac agcaactccc agaagcgccg ccgttgtatt
     9721 taatgagaac caaaccctct ggcaaacact tggctttagc cagtctcaag tttcgctatg
     9781 gcttgcctca ttgcctcaaa gtaatcatct aaaagggccc acaacggcaa cgtaccaggt
     9841 tatacctgat atcgcggcac atttggtatc attgttgcat caagctggtg ggcgcatgcc
     9901 ccttgcacaa gtactgaaaa agctccctgc tggtattacg accagtgaac agcagatacg
     9961 caaactggca cagaagcacg cgcagataga gattaaaggc cccttgctgg tactgataaa
    10021 ttagccggca tttgtagtaa ccattggctg tattaaagga ttttagaaaa catcatggca
    10081 aaagaagcaa aaaaacaaga cccgcaacag cagctagaag cactgttcaa taaactggtc
    10141 aaacagcact ccgagtttac tcagccattg gtcgaattac ccgagcatga attcgccaag
    10201 gctggtctca ttcaaggcct ggttgaaggc aacttaacgc caccagtcat gttgattgcg
    10261 ctgacagaag gccattggga atacgccgct gacactgcaa agtttgatga tttagcctat
    10321 cagctgttaa gtgatatcga aggtgactgg ccagtatatg ccgttgtgga cgatggcttg
    10381 aaccaccgta ttttggcctt gttcggtgcc gatggatctg acggtaccca cggtgcagat
    10441 accctgcctg gtctggaaga attacgtagc tttgatcgca tggagcgtga tccaaccttt
    10501 cgctggtcga tgcgtgttta tacacgctta atgcagcgat ttgatgcctt ccatgaaaat
    10561 gtttatcgcg ttaccaaaga caaggtaaac gacaagaacg acattattga ggaagtagct
    10621 aagcttctgt ttttagaaac tttccgcctg catcacgatg aagaccttac ctttaaagat
    10681 gatgagggaa ataccctgcg ttttcgtgat gtctttaatt ggcaatatgt ggagagccac
    10741 ggcgataaag ccgttgccca aatcaaagcc gcctttgagg aatttaaaag ccacgaaaac
    10801 tacgttgtga tctcagatga tggctcacgt aaccccatct ttagtaaaga aacacacctg
    10861 cggctgtctg ttgcaaaaaa ctatcaggat ttgctggaag caatacaaaa cctgggaccg
    10921 gtaaaaacca acgacggtaa aatcgctaaa gaacacggca cgttggctga tgtgtcagga
    10981 gatttactcg gcagagtgtt tgatgtattc ctacgcgcta actttgaatc caaaggtggc
    11041 cttggcgttt atttaacacc taacccagta aaacaggcca tgctagaaat tgctatgcac
    11101 gacatcgacg atgacgatga aatgcgatcg cgcttagcca atggcgattt tcgtttctgc
    11161 gaccccacct gtggctcctt tggttttggc tcggtggcgt taagccagat tgataaatgg
    11221 atcgatttta agctggtgct ggccgacgat aaaaaagaat cattgaaaca aaaattacgc
    11281 gactgtgcat ttaccggtgc cgatgctgca ccacgcatgg taatgctggc ccgcgtgaat
    11341 atggcattgc aaggtgcccc caaagcacag attttctata ccgacaactc actcaccact
    11401 aacgccctga agccgaatag ctttgacctt atttgcacca acccgccgtt cggtacgcct
    11461 aaatttgata aaggcaaaaa tggtcaaaac tcaaaagcca attacgaagc caatatggag
    11521 caagtgctcg gtggttttcg tccgaccaaa aaagtcgttg atagctacaa cgcctggtat
    11581 gaccacgtaa aaatgaagtg ggaggatatc ggtgatttag agctggatga aaacggcgag
    11641 cctaaatggg cgggctatcg taccgatttg cgcagaacgg gcgggactga taaaaagccg
    11701 ttttacagcc tgcagcctac aaccgcaggg ctcgcgctgg gcagcaagcc cgatagcaag
    11761 ggcaactggc aaccggtagg tgccactatt gacccagcag tactttttat cgaccgctgc
    11821 ctgcaactgc ttaaacccgg aggccgtctg ttaatagtgc tgcccgatgg tattttatgt
    11881 aactccggcg accgttatgt acgcgagtac atcatgggga aaaaagacga aaaaaccggc
    11941 gagtttgttg gcggtaaagc cattgtaaaa gcagtaattt cattgccgag cgactgcttt
    12001 aagctctctg gcacgggcgc caaaacctcg attttgtatt tgcaaaaacg ccatgctaac
    12061 cccaaccagc cagagcaatt tttgcccgag ccacaaactg acgtgtttat ggcagtggct
    12121 gaaaccttgg gctacgtggt gaaaaataat attgaagact ataacgctgg tgtcgcaaac
    12181 gatctggata agattgtatc ggcttataag cggggtgagt gatggacaaa ttttttcgaa
    12241 caaacagctc aattcttgtg gaaagacttg atcctaactt ttatcaaccg agcctagtag
    12301 aaaatgacaa actgataaaa tccaaaggct gggtggaact ccagtcactt tatgaagcga
    12361 gtggtattgg aaacacgtca gcagtcgaac ggtattactc tgaagctgct gatagtattc
    12421 catatatttc gggaaaggta attaaaagct tcaatattga tttagatgaa tgccaacgaa
    12481 tatctctcga ttcacataaa aatgagctta caaaatcagc gctcaaacca acagatgttt
    12541 tagttattag aaaaggagat atgggaaatg cgtgcgtagt tccatctgaa gttaacgaag
    12601 caaactgttc ttcagaagtc atatacctga aaatgaaggc ctctagcgat ccttactatc
    12661 tcgttagcta cctgaactgt gaccaaggac aaaaggcttt taagcgacta ggccggggga
    12721 ctattattcc tggagtgagc ttgttagatg ttccacgttt acctattccg aaagtgtctg
    12781 agtttgttca aaaatacatc ggggataaag ttcgccaagc cgagcagctg cgcgcttggg
    12841 cgaagctgct tcgcacatca gttgacgctc atcttaatag tctaaatctg ccgatcaacg
    12901 agccccccgc actattaaac cgagtcagcg cgcaaacaat ggaggatcga ctcgatccac
    12961 gtccttatcg gactcactac ctatgtttag tgcgtgaaat tgaaaaacta cctcatgaca
    13021 gcattagcac tttggttgaa ttggcatctg gttgcccagt atccagtaac gactttttgg
    13081 aaaactcagg cataccttta gttcgcatcc gcaatatcgg ttttgatgat tttattggtt
    13141 tagatactgg cgttagccaa gatgtatatc aggatgcgac taagtatcaa gctaaagaca
    13201 aaatgatcgt tgtcggtatg gacggtattt tccgctcgca gtttttcata tctgacgagc
    13261 tacctatgct ggttaatcaa cgggttgcaa tgctctcacc tcaaaatatt cgtggcgaac
    13321 tattaacaca ttggctaaat cgccccgaag gacaaatgca gctaaatcaa tgggcagtta
    13381 aaacaactgt agaacatact tccctatctg atattggtcg cgtactaatt cctcgactgg
    13441 ataaaagctt agaaaacaag cttgctgatt atttgctcaa tgcgaggctg gcttatcgtt
    13501 atgccaaatt tttaacacaa gtagccaaaa ccctcgtcga agccctcatc gaaggtcaac
    13561 tgaccgagca acaattaatc caggctcaac aagccctgga agatggcgac aacagctttg
    13621 atcaagcgat tttaagtaaa ctgagcgctg agggttatgc catagaaggc gccacaccgc
    13681 tatttagcga tgtggatgag ttatacagtt tgttggaaga ggcggcgcaa gcggaggctg
    13741 aagagtaaag atggagacaa ggggcatgga cagcattaca accaggcaaa ataacgaccc
    13801 cgtaaatagc gaagcggcac ttaacctctg cctgcagctt tggcatcaag gcggtttaac
    13861 cgccagtaaa gcagcgttgt tactggcggc tgtgcctgcc ctcaggtcac tgttacaacc
    13921 aataatacag ccgaaaaaga aagacgctga aacggatatc attagcgcct ttagcctcac
    13981 tgcaccatta cttgatgcct ttagtgattt aagccagtcg ggtgaatggc aattagcgtt
    14041 gttgggctta aacccagatg ttcgccaaca ttggataaat ttagccgccg ctcgctgcca
    14101 ggaagctggc gcgatgagtg acccaatggt attggttaaa ctcattcagc aactgggcaa
    14161 tgccagtgaa tgggtcttag cccagttgga aaacgcagac ttctcaccac aaatcattgc
    14221 aagcccattg gcgcaaaccg aacgggattt actcggtcat agcctaaacg acaatgctgc
    14281 cattcccgct ctatgccgta ttttgcgcac cagccatacc ctgtttaccg tgagcgaaca
    14341 aaatgaacca ccggcatcta tacaagcggt ggatgtcacc gcaaaacaac tgacaaataa
    14401 ctggtgtagt gggcggttgt tagcactccc taataccttg cttgatgaac ataaccttaa
    14461 acccaatgcc gattggttat tggtgtcccg ctcgggtcat gacaacatgc cgcttaccga
    14521 gctattcgcc caacagccct ggttattttt actcagtcta attatctttg tgcaagatgc
    14581 ctgggctgcc gagcaacgtg gcggtttgtt gctcacctta cctgccgggc aaaatgcctt
    14641 tgcccccggt caaataaatt tggcggtaca gggcattgaa ggtgatgagg tgagtctggg
    14701 tagtttggcc gagtttcttg tgcttctgct cggtgagctc aatatacccc tctacccggc
    14761 actcgatgcg aacacggaaa gcatcaaccg cctaaaccgc gtcctgagct cattcattgc
    14821 cgaattgttg gcaaaaaaaa tctggcaatt taccgaggcg ggtcgtggag aggccggcca
    14881 atatcgaatt catacaagct tcagcgatgc ctgttatagc ctgccgttag caccactatt
    14941 tggctacaaa agccaaacgt tacaacggac cattaagcaa ctggcgcaaa actgctatgc
    15001 caataaaaaa cgcgccgcca atagaataaa ccttcaagga tcgtcgttat gagttttgaa
    15061 ttaaacgaac agatggttga tagcctggca aaacaacatc gctgggcggc gacgggtgta
    15121 tcagattatc cacctcgcga cccgttagtg ggccagagcc gcttttttaa acgctttcaa
    15181 acctttttgc atacggtgga tcacgacgat gatcgctttg cccatgtgtt tgccgtcgaa
    15241 gccgaatggg gtcggggtaa atcacgttta ggccatgaat tggtcgcaca aattaacgac
    15301 tgctccaggg gctggtttgt ccgcgacgaa caaggccaac tgcacgataa gcagctgttt
    15361 aatcgcgaca cgcaagacaa atacctggcg ctctacattc gctactcgca agtggcatca
    15421 gattatcaaa actcggataa ctggtttgct tttggtttat atcaggcgct attaccactg
    15481 gccactcaaa cctttgatgg atcgattcaa agtgaaattg ccaaacaagc ctatgaacgc
    15541 ttaatgccat ttggctttaa atctgaactg ttagcagatg ccttacagct gaacgccaac
    15601 cacagcgaag aagacctcta tactgaccaa acgctggtag tcagtttggt gcaagccgcc
    15661 tataaagtat taaagagttt tggcgtgcag tatatgttgg tggtgctgga tgagctggaa
    15721 accgttgcag aagccgccac ctttggcctg gaagatgaac aagataaacg cctcgatggc
    15781 caggccattc gcctgattgg taaagcaatt aaagaggaag accctcgtcg taaacttcca
    15841 tggttgcgct atgtggcgct gtgttcacca ctgcttggcc agcaattgcg tgaaattcaa
    15901 tcggtcgccc gccgctttga gttagtggaa cttgagcata atgcctttgc cgatgtatca
    15961 gactatgtgg caagcctctt aaaagacggc aagctgagtt ttaattaccc tgctggttta
    16021 gtggaagctg catacgccat gagtggtgcc aacttcggtt ggtttaacgt ggtcatggct
    16081 aatgtggatg cagtgctgag ccaataccaa caagcaggcc gcagtatcaa ggatgtgggc
    16141 gatttatttg aagctgtgct ggcaggctct ggccgcgtag ccaggcatgt gctggataaa
    16201 ggcgcgatag aaggcattca aactcgcgac caggcactat tgaccgaatg tcgtagtttg
    16261 ctctacggcc aattacccgt gcgcttagct gaaatgggct atgccacaga attgctggcg
    16321 ctgaaaaacg aagatctgga acccatagcc gctcattacc gcaaactgag cattgaccgc
    16381 ctacagtgcc gacaagccct ggagcaagcc aaattccgcc gcgataaaga tgaatggtac
    16441 taccctgcag tagatcaagc gctgaactta gatactttgc tggataacct gcgtactttt
    16501 tcaattaaag aacaagccgc aagcggcggt aaaggcgcgg tattactacc tctgtcaaaa
    16561 ggtgagttta aatacttact cagcttgctg tatgaccacc cagcgattga attcgctgcc
    16621 gatgccttat ggcacaagct agtgggtgaa actaccgaac tggacgccag cgaagcaacg
    16681 catattggcc ccagtgtggc gatgttactg cgcctggatt tgcgctatcg cagtgcccag
    16741 caaaactcac tgatttttaa agatcccgcc atgggcgacg ctcatgaaca ggcaatgagt
    16801 gaacttagca aacaaagtag ccagaagccg ctcatcaagg cattagcccg tgtaacgggc
    16861 tttgtgagat tacttgataa caactggacc tatgatgaaa acttgctgag taataaagcg
    16921 ggcagcgacg atgcaccttt ggccatttta accgagcccc gtggccgcca gggtggttta
    16981 cttaccctgg atggatttaa gcttcaccca gaaggtaaag ccttgtttgc ctgggtcagc
    17041 aacctaattg agctgaacaa tcttcacacc tttgcggcaa accacagcca ccaaaatggt
    17101 cgcacgccag tattagcgtt aactgcttct gcacatctca tggagcaata cagccgactg
    17161 gatgaaagaa atgagttgcg tgatgcgata ctgctgcatt atgtcaaccc aagcgaagca
    17221 gaccagttag agcgtattgg cttaagtttg gcagcatgtc agttgcacgg cgtgaattta
    17281 actgccgaca gctttaccgc gaaattcaaa aacaagctgc atgccctgac aacctttgcc
    17341 acggatgcca ttcacaagtg gcgccagcgt ttgcagcagc gcggtttaat tgcctggccg
    17401 ttaaaggtag atggtaaatt atcaccgaat gaccgagacc tgttgtttaa aggctggtat
    17461 cagctggcca ttgctcaccc tgagttaaat ggcattctcg atttacagca gcaacacggc
    17521 gtgccagtga atgagctgag cagcttgttg gataaactta aggtgccggg cagctatatt
    17581 gccaaaggtt acactgccga tgaacatgcc gggctattta ctgaacttaa taatgtgcag
    17641 cgaagtcagg cgcagatccc attattttta gcccgtattg ctcaccctaa taaaaagcac
    17701 aagtggcaat tcgaaggctt taagcaacag ttttatttcg cctatgtggc tgaaacgagt
    17761 gttaccgcca aaggcgtgtt taatgactgg atgtggtggt gtggagagct gaacttactc
    17821 actttaacca atccaacaga aaaacaggca atttgggagc actacccgcg ctcccgctta
    17881 gaaaatgcca ttagagaagc gcaaaactgg ttccgtggta acgatatggg cagctatgca
    17941 gccaatgtgg aagtcatgag ccgcgtgtat ggttatgcac gcattaatga aatgttcgcg
    18001 ccactgggta aaaataagct gggtttcgtc accgtggaag ctaaagagca gctggaaaaa
    18061 gcacaatcgc tatttaacgt gctcaaacag caagaagaac aattggctga tatggtcgag
    18121 gccaacgaca cgaaagtatt ggcagggctt atccacaaac gcgctgaagt gctggagctg
    18181 gtagcaaagg ttaaaccgct taatagttcc aggcctatgc taaaagatgc ccatattctc
    18241 agcttagaag acaaaacgac ctcgctttat caacggattg aacaggcctg tttatttgcc
    18301 gagtttgttg agtgcagcgc tgagcgtatc aacaaccgac tcgttgattt aatcatagat
    18361 gttgaaacag agtgtgcccc gctgacgaat tttccgaaac gtctgtacac caacacgctg
    18421 cgcaccatag gtcatatttt agatggagcg ttaaaagacg ataccagcag tgccactggc
    18481 cgaaaagagc agcaagcaga tagcgatacc ttactgcatt acctgcgtaa actcgattta
    18541 ggtagagcac atgacaagct ttcagcctta gcgcaagaag tgggtttaaa cctgcacaat
    18601 gatcaacagt tgccgatagc ggagattcag gggcatattt tgtccagcta ccgcaactgc
    18661 aaagaacgat ttagtaagct ggttaataac ctcacagagc aaaaactacg tgcacaacaa
    18721 ctgcaagaat ggcttagtag cgccacggct gagtatcagt tcgccgatga cattgcagag
    18781 ctaccaaaac tggtaatgaa attacagctc attgaagatg ctattgccga tctccccagt
    18841 gatgcagaga gcaagcgtca aagtatgcaa aacagtttgc gcaatgggca gttttcatcg
    18901 ttacgggatt taccagaaga gcttttaaaa ccggcacgcg gtcaacttac accgattcaa
    18961 ggccagctac ttaaaattga agagcgtcta aaccaagtaa gacgcaactc catcgcacag
    19021 gtaaacagct ggctgccgtt attaaaaccg ctattggctt ctcaaaagca agcggaacca
    19081 gcagcgttga ctctggagga tgtgagcaat ttgggtgtca gagaacttca acaggtgtgt
    19141 gaaagcaccc aggccaaatg gcagagtcaa ggtgagcaaa tattaaatga tacaggctta
    19201 acactggcgg attggcaacc gatttataag gctttgagcc aaaatcagga acccgtgtta
    19261 acacctgagc aacagaaagg acttgtagac aaaggcattg taaaaatgcg ccttaccttt
    19321 gccactggtt tgtagaggtg acgcgatgag tcgttattta tctgcggcac tggccagtaa
    19381 tcgtaaaggc cgttttttgc aaactgttgc gggggctacc cccttaatga aggattggat
    19441 tagctcgccg ccagccagcg gtttgttaat agtgcaagca gaagagttga ctgacgctaa
    19501 tactatgcag cacctgtatc actgggctat gcaggcaggt tgtgccgctt tggtaataaa
    19561 cctgaaagca gaacaattta ccttattagc gcaattgccc tatccactgg attggcagtt
    19621 agtaccagcg agcttgaggg ggcaggaacc ggggctaacc gctttgctag ccagtgaaac
    19681 agaccaggct attgctggct ttactggcag cgcagatcgc taccagcacc aggccggtga
    19741 tgtagtgcat acccgctaca tacgaaaaca cagcaacagt gggctattag cctttaccac
    19801 gttacctttg tggtcgctga ctttactcga tcatagtgag ctcctggtga gctggttgaa
    19861 ttggtttgtg gaccatgcag gcattgctga acgaataatc gaacctaaag cgccatcaac
    19921 tgactacacg ccagataagc acgatctggt tgtattgcta ttactctacg ctggcggtgg
    19981 catgaactta caagcgttgt ctgagcataa tgcggttaag ctgatgtttg atgttaatag
    20041 ccttgatatt gtcaagcggg gtgagatgtt gaggcagcac gattttatcg acgacgcggg
    20101 cataacagct accggtaaaa catgcctaca agcctcgcaa tattgggctt atgccccatt
    20161 acttggcgag cagctacaca caggaacgct ataagcatga atgagaacaa aacgatgaat
    20221 ttagcgcaat cgctcgccat cgatagccag cttcgcttac cgggaagtgc aggacgcttg
    20281 cttaaacagg tccgtgatat tgagcaagtg gcgccgctat tccgtgatgc tggcttagtg
    20341 aaagcggtcc ccaagcccac aattgttaat ttacgcgtag ccggtattgc gactaaatcg
    20401 tgcttagaca aggtattggg tggttttatt tatgccagtg cagcagtgcg cacagatttg
    20461 ttaatagatg acaataaagt cagcacggtg ggtagcgact cggtatcaga actggatgat
    20521 attgattttg aagctcagcg taaacggctt gatttaattg aaattcgtca ggcttatcag
    20581 ttagttgaga aaaaactttt aggttatgag cctggcgatt taattttgct ggatacgccg
    20641 ttgtttttag cacgggatat ggctccgctg gagagaaatg tccgtcataa acaagagtac
    20701 gaaaaaacca gacaagtgat agaaactttt tggcaaaacc atcgctccag aatttttccc
    20761 tggaatcaaa atggtgtagt acttgcgagc attttggctg agcgctttag tgccattgta
    20821 agtatcgcca aacaagattt acgtaccgaa caaggacgca aacaaatttt agcatctgat
    20881 gggttttcta aagagttgat gcaaaatctg gaaggactgg atgaaagttt ggttggcgtg
    20941 ggagatttgc gatttatcaa tggcatttta ggcaactatt cgcgcaccat cgcatttcgt
    21001 ttgtctgaac aagaaaagcg cattgaaccc aatattgaag ctaagcaagg tctaattggt
    21061 tttcacttcc gtagtgcccg aggtggacag ataaaaatgg tgcaattagc gggtgacgaa
    21121 ccagaatgga aaagtgctga tctcgattta gttgcctctc gcctgatggt gctggatatg
    21181 caaaacaaag gtaatgcaat gccgttgcca caattacttg gttatcaaca gcttggtatt
    21241 ttacctaaat ttgctacgtt ttatcgccag ggtttacacc aggcactaaa gaataatgat
    21301 attgaggcag ggtggttggc aggcctcgat gagggattga ataatggctt ataaagtttt
    21361 aggtaaatta gtcggtaaca ctggtgatgc cagtcagtta actatggttg tacaagactc
    21421 gttttcagta cgccgtggtg agtttgtacg cattatgcac caggaacgca aagatgaggg
    21481 gcaagtcgcc gtactggggc gagtgaccaa gataagccgt actaatatgc tctataacgc
    21541 cggctttggc gatggtgtaa ccgagcttga gcttttgccc ggtgcgaatg taactggtga
    21601 gaatatgttt gctcaggtcg agctggttgg ttaccgcgat cctgttactc gtcaaatcaa
    21661 aattccacgt cgcccgctta acccgggtac cgctcttgaa accgtcgatt ttcagttttt
    21721 gagtgatttt tacgaattta atgaacactc cagtttgcac ttaggtaact tagtaggcta
    21781 cgacaaaggt gagaatacag ttcctgtttt tattgacgtg aacaagttgg tcactgagca
    21841 tttggctgtt ctggcaatga cgggttcagg taagtcttat acagttggcc gcattattga
    21901 gcgtttagtt gcggttaata acggtacggt agtagtattt gacccacatg gtgaatacgg
    21961 taaagccctc gctggcggta atctgcaatt ctcaagcttc ttagaagcca ctgacgataa
    22021 gcgtgatcaa gaagcactgc cactgataaa acagaccttt gaaaagctgc aagccgccgg
    22081 tgcaggtatt caggtttact cgccgcagca tgaatctttt aaacataaat acgccagtaa
    22141 gaatacgcca ttagcgcttc agtttgacca ttttgaaatg gacgatattg ctgaaatatt
    22201 acctggctta acggaaccgc agcaacgagt actcgatgtt gcaattcgtt attggcgcta
    22261 tgcagataag actgagccac gagatattaa ccgcctacgc ttcttattag gtgatggtat
    22321 tgaagagtta aaagagtggg acgacctttc tgaagctgag gcgaaggcgt tatcaggccg
    22381 tagtgccgcc gtcgcctcga tgaagctttc acgtgtgctt aatgaagcta aaagttttta
    22441 cagcgcaaca atggctgagc caactgatat ttacaaaatg gtgggtcgcc cgagcaatca
    22501 acaggggcgt ttagtcgttg ttgacttaca aggtttgagt gataccgcta agcaagtcat
    22561 ttgtgcactg ttatctagtg aaatattaaa agcagcctcg agcaaaacgg acccgctgcg
    22621 cccatgcttt atcatttacg aagagggtca taattttgcc ccggccggcg gcaacgcagt
    22681 cagtcaccgc attattaaaa agatcgctgg tgaaggtcgt aaatttggtg ttggatttgg
    22741 cattgtgagc cagcgtccat cgaaattaga ttcagatgtg acctctcagt gcaatacgct
    22801 aattacgatg cgcttaaaga accctgacga tcaacgattt atagctaaag catcagatat
    22861 ggtgagcaaa gctgatctgg aggaactccc gagtttatca accggtgaag ctttggtgtg
    22921 tggtcgttca atccctgcac cactgcttgt taaagttggt agtaaggcat taattcacgg
    22981 tggtgaatca ccagaggtac tgcgtgtttg gggctctttc agtggctaag gtgcctatgc
    23041 ctgaagttgc ctgggtaaat gagacatttc cacttttagc aagcggtaag ctattggacc
    23101 aggatgtatt ggtccacagc ctgggctgta gcgtttggaa tacattcggg catgaacagt
    23161 catttatggc ggtagtggaa tgcccggctc cagggacatt tggtgctgat attcgctctg
    23221 actcgggctg gtttcataaa tcttctgcat cgcctgtgtg tttaatcgaa tttgaacgct
    23281 ttgatggttc agcgaagggg cagcaaaagc tggaagaaaa attaaaaaac ttactggaag
    23341 cagcacagcg ctggaatcat tgccccaaaa ccctggtcct cagtgcctgg agccagggct
    23401 tagtaggtgt acctgatact caaaaactga aagacatatg ccgtatgggt tttacttcgt
    23461 ccacgggtac ccaggtaatc gccgctcctg atgttgaggt ggtatttagt cgctttttat
    23521 ttatcaaaaa cctgaacatg attgttttgg acagaattca ctatgaggtg ttgatgtgaa
    23581 tataaaatca gcacattttt tgccagggac gaataaacgc cttggtgaca aatacctgtt
    23641 acttgaatgc cttggtgatg gcagccatgg ctgggtatgg cgggcggaac gcctgaaaga
    23701 tggaaaaatt gtcgcagtaa aaatccctaa ggatattact cgggaagacc gtcagcttgc
    23761 cgaaggtaaa gagcttttaa acgtcgagcc gcacgaaaat attgtgcaaa tttttgatat
    23821 tggccgtata gataatgaat ggttctatat tgaaatggag tattttccca gccaaaccct
    23881 ggcgcaaaag ctcgatgaca ggcagcgtaa ttttggccag acctatgaac ggttgttcag
    23941 aatatatcgt caagtgctct gtgctgtgca ttatttggcc gaattaccag taccaatatc
    24001 tcatggagat attaagccac ataacatttt ggttggagag cgggacctgg tgaagttgac
    24061 ggactttggc agctcagctt taccagaaga aatttacgtt cgtacccgtg aaaatggcgg
    24121 gactgtgctg tattcggccc cagagttctc caacgtcgat agccgtagag gcagcctgga
    24181 agagctacta ttgggcgaca tctatagctt aggtgttttg ctataccagc tgctgacagg
    24241 aaagctgccc catgatacgc cagcacaagt tcagcgccac gcacctttca agcttccgac
    24301 agagattaat agctctattc atacggacct ggaacaagtg gttcttactt gtttacaaaa
    24361 acgagcggag gatcgctttt ccactatttc cgacttaatt catgcatttg attctgctgc
    24421 cactaagcaa ttaaaagttg gtgctattgc tcccgttttt caaactaaac ctgctcagga
    24481 ctggtcaagt gcggtacttg aagcaatgtc cgatcaatct taccaaaaag ctgctcaatt
    24541 agcgtcgcag gagtatagcc gcagtaaaga tctacaagct ttacatcagc aacttattgc
    24601 cttgtatcga gccaatcgct tattcgactt tgaaaaagtt gtagaagata ataaagcgat
    24661 cttacttgag ggtaaattga ctcaaccagc agctttgttc gagttgatag ttaaggcaaa
    24721 cctacagtta cgcaatatca gtcaagcaaa tttctggctg ttgcaacgaa agcaagctga
    24781 agcagaaaac gcggccacca tgtatttaga atcatctatc cttggccttg aagcgaaata
    24841 ttctgaagca agagtactat tggagaaagt aaaccaaaca actccaatga agttccatgt
    24901 gttaagccaa ctcattttag tttgcgagca aatgcgtgat tactctggtg cagcggctta
    24961 tttgaaagcg gcattgcgag tagcaccatt ggataacagc atgaaagaga aaaaagaatt
    25021 gtatgcaaag cttggagtta tttgattgaa aggatgacca ttgcgcgaac aaggcatagt
    25081 ggatatttaa cacttccgtt cacataccgg acaaaaaaat aaagttttac ataacgattt
    25141 ccttcttgat tgagttaaga caaaagcctc tccccatt
//
LOCUS       DAZKIV010000059        25146 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZKIV010000059 DAZKIV010000000
VERSION     DAZKIV010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.059"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..1650)
                     /gene="ybiT"
                     /locus_tag="ACHOT6_002340"
     CDS             complement(58..1650)
                     /gene="ybiT"
                     /locus_tag="ACHOT6_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFP9947545.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1869..2789
                     /gene="ldtB"
                     /locus_tag="ACHOT6_002341"
     CDS             1869..2789
                     /gene="ldtB"
                     /locus_tag="ACHOT6_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9947546.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2848..3966)
                     /gene="ybiR"
                     /locus_tag="ACHOT6_002342"
     CDS             complement(2848..3966)
                     /gene="ybiR"
                     /locus_tag="ACHOT6_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFP9947547.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLDNVS
                     HLSDPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDHRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3963..4430)
                     /gene="mntR"
                     /locus_tag="ACHOT6_002343"
     CDS             complement(3963..4430)
                     /gene="mntR"
                     /locus_tag="ACHOT6_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFP9947548.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4616..4744
                     /gene="mntS"
                     /locus_tag="ACHOT6_002344"
     CDS             4616..4744
                     /gene="mntS"
                     /locus_tag="ACHOT6_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFP9947549.1"
                     /translation="MNEFKRCMRVFSHSPFRVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5016..6599
                     /gene="opgE"
                     /locus_tag="ACHOT6_002345"
     CDS             5016..6599
                     /gene="opgE"
                     /locus_tag="ACHOT6_002345"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFP9947550.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6648..7163)
                     /gene="ompX"
                     /locus_tag="ACHOT6_002346"
     CDS             complement(6648..7163)
                     /gene="ompX"
                     /locus_tag="ACHOT6_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFP9947551.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7216..7281)
                     /locus_tag="ACHOT6_002347"
     CDS             complement(7216..7281)
                     /locus_tag="ACHOT6_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947552.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7516..8403
                     /gene="rhtA"
                     /locus_tag="ACHOT6_002348"
     CDS             7516..8403
                     /gene="rhtA"
                     /locus_tag="ACHOT6_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFP9947553.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            8702..9205
                     /gene="dps"
                     /locus_tag="ACHOT6_002349"
                     /gene_synonym="pexB"
     CDS             8702..9205
                     /gene="dps"
                     /locus_tag="ACHOT6_002349"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFP9947554.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            9609..10355
                     /gene="glnH"
                     /locus_tag="ACHOT6_002350"
     CDS             9609..10355
                     /gene="glnH"
                     /locus_tag="ACHOT6_002350"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFP9947555.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            10494..11153
                     /gene="glnP"
                     /locus_tag="ACHOT6_002351"
     CDS             10494..11153
                     /gene="glnP"
                     /locus_tag="ACHOT6_002351"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFP9947556.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            11150..11872
                     /gene="glnQ"
                     /locus_tag="ACHOT6_002352"
     CDS             11150..11872
                     /gene="glnQ"
                     /locus_tag="ACHOT6_002352"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFP9947557.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            11989..14214
                     /gene="ybiO"
                     /locus_tag="ACHOT6_002353"
     CDS             11989..14214
                     /gene="ybiO"
                     /locus_tag="ACHOT6_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFP9947558.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGESLSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGATPAEPPPPGEPT
                     L"
     gene            complement(14211..15137)
                     /gene="rlmF"
                     /locus_tag="ACHOT6_002354"
     CDS             complement(14211..15137)
                     /gene="rlmF"
                     /locus_tag="ACHOT6_002354"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFP9947559.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            15413..15673
                     /gene="mcbA"
                     /locus_tag="ACHOT6_002355"
     CDS             15413..15673
                     /gene="mcbA"
                     /locus_tag="ACHOT6_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFP9947560.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            15937..18219
                     /gene="fiu"
                     /locus_tag="ACHOT6_002356"
     CDS             15937..18219
                     /gene="fiu"
                     /locus_tag="ACHOT6_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFP9947561.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPTGVAKGSPVTTVDTAK
                     SGNLMNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            18261..18938
                     /gene="ybiX"
                     /locus_tag="ACHOT6_002357"
     CDS             18261..18938
                     /gene="ybiX"
                     /locus_tag="ACHOT6_002357"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFP9947562.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTCSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            19054..19278
                     /locus_tag="ACHOT6_002358"
     CDS             19054..19278
                     /locus_tag="ACHOT6_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DksA/TraR family C4-type zinc finger protein"
                     /protein_id="HFP9947563.1"
                     /translation="MNSTIEDAIARARGEIPRGESLDECEECGAPIPQARREAIPGVR
                     LCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            19543..19803
                     /gene="ybiJ"
                     /locus_tag="ACHOT6_002359"
     CDS             19543..19803
                     /gene="ybiJ"
                     /locus_tag="ACHOT6_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFP9947564.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(19906..19965)
                     /gene="ybiE"
                     /locus_tag="ACHOT6_002360"
     CDS             complement(19906..19965)
                     /gene="ybiE"
                     /locus_tag="ACHOT6_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFP9947565.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(20031..21116)
                     /gene="hcxB"
                     /locus_tag="ACHOT6_002361"
     CDS             complement(20031..21116)
                     /gene="hcxB"
                     /locus_tag="ACHOT6_002361"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFP9947566.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGRTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQGFCQQLAS"
     gene            complement(21257..22219)
                     /gene="ybiB"
                     /locus_tag="ACHOT6_002362"
     CDS             complement(21257..22219)
                     /gene="ybiB"
                     /locus_tag="ACHOT6_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFP9947567.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAIQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            complement(22247..24397)
                     /gene="dinG"
                     /locus_tag="ACHOT6_002363"
     CDS             complement(22247..24397)
                     /gene="dinG"
                     /locus_tag="ACHOT6_002363"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFP9947568.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAINDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILDLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            24517..24999
                     /gene="ybiA"
                     /locus_tag="ACHOT6_002364"
     CDS             24517..24999
                     /gene="ybiA"
                     /locus_tag="ACHOT6_002364"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFP9947569.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
BASE COUNT         6121 a         6684 c         6212 g         6129 t
ORIGIN      
        1 tttgcatttg tcatcagccg ccgtcacccc gacagtatcg gggtgacggc taatcactta
       61 ctcgatccct ttactacgca ggtaatcttc gtaattaccg ctaaagtcga tcacgcgttc
      121 cggggtgatt tccagaatgc gggtcgccag ggagcttacg aactcacggt cgtgtgaaac
      181 aaagatcagc gtgccctgat acagttccag cgccatgttc agtgactcga tggattccat
      241 atccaggtgg ttggtcggtt cgtccatgat cagaatgttc ggcttctgca tcattaactt
      301 accaaacagc atccgccctt tttccccacc ggaaagcact ttagctggct ttttgatgtc
      361 gtcctggctg aacagcaaac gaccgagaat gctgcgtacc gcctgctcgt catcgccttc
      421 ctgcttccac tgactcatcc attcgaacac ggtcagatca ttttcaaact catattcgtg
      481 gtcctgggcg tagtaaccaa tgcgcgcgtt ttcagaccat tttacggtgc cgctgtccgg
      541 ttgcagatcg cccaccagcg ttttcagcag cgttgattta ccgacgccgt tggtacccag
      601 taccgcaagt ttttcaccga cctccagcag caggttgaga tttttaaaca gtggaccgtt
      661 atcaaaccct ttggtcagac cttccacttc cagcgcgtta cggaacagtt tcttatcctg
      721 ttcaaaacgg atgaacgggt tctgacggct ggaggctttc acctcttcca gtttgatttt
      781 atcaatctgg cgcgcgcgcg aagttgcctg gcgagatttc gaggcgttgg cgctaaagcg
      841 gctaacgaaa gattgcaact cagcaatctg cgctttcttc ttggcgttat cggccagcag
      901 acgttcacgc gcctgggtcg ccgccgtcat gtactcatcg tagttacccg gataaacgcg
      961 cagctcgccg taatccagat ccgccatgtg ggtacagacc atgttaagga agtgacggtc
     1021 gtgcgagatg atgatcatgg tgctgtcacg ctcgttcagc acctgttcca gccagcgaat
     1081 ggtgtcgatg tcgaggttgt tggtcggttc gtcgagcagg agaatatccg gatcagcaaa
     1141 cagcgcctgc gccagaagca cacgcagctt ccagccagga gcaacttcac tcatcgggcc
     1201 gtagtgctgt tccactggaa ttcccacgcc aagcaacaat tcaccggcgc gagcttccgc
     1261 agagtaaccg tccatttcgc cgtatttaac ttccagatcg gccactttat agccgtcttc
     1321 ctcactcatt tccggcaaag catagatgcg gtcacgctcc tgcttcactt cccacaactc
     1381 tttatgcccc atgatcaccg tatccagcac agtgaactct tcaaaggcaa actgatcctg
     1441 acgcagctta ccaatgcgct cgttgggatc gagggaaacg ttacccaaag tcggctctaa
     1501 gtcgccgccg aggatcttca taaaggtgga tttaccacta ccgttcgcgc caatcaggcc
     1561 gtaacggttg ccgccgccaa atttgacgga aatgttttca aacaacggct tactgccgaa
     1621 ctgcatggtg acgttactgg aaactaacac aggcgtatcc tgaaaagaga tatgacaaac
     1681 cgcgcattat gccataagtc tgaaagaaga tcctgctcct cgcttaaggt ctacactcaa
     1741 gccaaaggca aaaaatgtga tttcgtacac atctgatttc actgtgagct ggaatgaact
     1801 tataatgcgc ttccaatact ctaatattct caacccaatg gcctgccagg cacaaaatct
     1861 cgcttaacat gaatatgaaa ttgaaaacat tattcgcagc ggccttcgct gttgtcggct
     1921 tttgcagtac cgcctctgcg gtaacttatc ctctgccaac cgacgggagt cgcctggttg
     1981 gtcagaatca ggtgatcacc attcctgaag gtaacactca gccgctggag tattttgccg
     2041 cggagtacca gatggggctt tccaatatga tggaagcgaa cccgggtgtg gataccttcc
     2101 tgccgaaagg cggtactgta ctgaacattc cgcagcagct gatcctgccg gataccgttc
     2161 atgaaggcat cgtcattaac agtgcagaga tgcgtctgta ttactatccg aaagggacca
     2221 acaccgttat cgtgctgccg atcggcattg gtcagttagg caaagatacg cctatcaact
     2281 ggaccaccaa agttgagcgt aagaaagcag gcccgacctg gacgccgacc gccaaaatgc
     2341 acgcagagta ccgcgctgcg ggcgaaccgc ttccggctgt cgttccggca ggtccggata
     2401 acccgatggg tctgtatgca ctctacatcg gtcgcctgta tgctatccat ggcaccaacg
     2461 ccaacttcgg tatcggcctg cgtgtaagtc atggttgtgt gcgtctgcgt aacgaagaca
     2521 tcaaattcct gttcgagaaa gtaccggtcg gtacccgcgt acagtttatt gatgagccgg
     2581 taaaagcgac caccgagcca gacggcagcc gttatattga agtccataac ccgctgtcta
     2641 ccaccgaagc ccagtttgaa ggtcaggaaa ttgtgccaat taccctgacg aagagcgtgc
     2701 aaacagtgac cggtcagcca gatgttgacc aggttgttct tgatgaagcg attaaaaacc
     2761 gctccgggat gccggttcgt ctgaattaat cttcaaaact taaagcaaaa ggcggactca
     2821 taatccgcct tttttatttg ccagccccta gttggccggg agtataacta acaaaacata
     2881 tcccaccaac gccgcccaca acagcatcgg tattgaatag agatggaaac gccaccagat
     2941 gcggtgatcg ttcgccatac gtagcgcaat caaatttgcc agcgatccgg gtaataaccc
     3001 aaagccacct acgtttaccg cccataccag taataaagac ggcggcacat agttcagcaa
     3061 caatatggta ctcggcacgt tactgatcac ctgcgataaa ccgattgccg ttaaccataa
     3121 cccggggtca gatagatgac tcacgttatc caacacgcct tgcaacgctg gaagctgggt
     3181 cagtaaatgg acgtcgataa acatcgccat aaacaccagc agcagcgtcc agtccacact
     3241 gagcaccacg cgacgtgcca gcagcgcaaa gcctgccgcc acaatcacca gtccccacag
     3301 ctcttgtttg aactccagcg ccgtcagaaa gacgatatac agccccagac aactccacac
     3361 cagccgcggt ttccactccg gtgtttgcac ccccgtatgg tattgcagcg cctttccagg
     3421 gaaacaacac cagcacagga gcaggagcgt cagcatcatt gcgccagcca gcggtgccat
     3481 ttgggcaata aatccggcaa acgaaagacc agaacgtccc cagataagaa tattttgcgg
     3541 gttgccaatt ggcgtcagta gcgaaccagc attgactgcc agcgcctcaa aaataatcag
     3601 ccgattaacc gggatctcac acagtctttt tagcgtgata gtcagcggaa caacaataaa
     3661 cagcgcgaca tcgttggtca gaaaggtaga aagcagcgcc gccgccagca ccataaacat
     3721 cgccagccga cgctccgtag caaagcggcg caccattttg cgccccagca catcaaaata
     3781 accgcttaac tccacacctt tggtcagcag catcaggccg cttaaggtga tgatggtgtg
     3841 ccagtcgata gcagcaggcc aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat
     3901 accaacaaga attaataact gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag
     3961 gctcatttgg caccgtgttt ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta
     4021 acatggtgct ccatgccttc cgcgtcgcga cgggcgattt ccggactgac gcccaacacc
     4081 aacaagaaat tttcgactat ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct
     4141 tctgccgtta aaaacacgcc acgccagggg atcatttcaa tcagccccat ggttgccagc
     4201 cgcttaagca ttttagccac cgtcggttgc gaaactccca gacgagcagc catgtccacc
     4261 tgacgagctt cccccacttc cctgatcaag tcagaaatca gctcaacgta atcatcaata
     4321 agctcgcgcc gatgcgcctc tctgacctgg cggaaccctt caacgtgctc ttccacgttc
     4381 actaactgcg tcactttttt tgctgttggc gtacctgcgc gacgactcat tgtgcttcct
     4441 cattatggtg acgcatacat agcgttctca tttcgtgcgc acattgtaaa ccagagttgc
     4501 gaaggtacaa aaaattaacg ttttagcaat agctatataa tatagcctgt gctatatctg
     4561 tatgtaatgc aatcatccct caaggatcga cgggattagc aagtcaggag gtcttatgaa
     4621 tgagttcaag aggtgtatgc gcgtgtttag tcattctccc tttagagtac ggttaatgct
     4681 gctctctatg ttgtgcgata tggtcaacaa caaaccgcag caagataaac cttccgataa
     4741 atagcggcgt cgcggtacgc cgcttcactc ctgctttcat gcaggcataa cgcgttttgg
     4801 tctgaaaaac cccacttttt gtcggatttg caatcccctt cgcaaaagat ttgttcgtca
     4861 gtagttgacc tgaacggcgg ctcgctctat cttcttgcag ccctgcgtat attgcggctc
     4921 gcggatgcgg acccctttcc actcttcacg cactcttgca ggtattgacc cttgacgcca
     4981 gggtaagcac atggcgtttg ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc
     5041 ttgttacccg tagccgggta tttagcccgt ggactgcgtt ctacttttta cagtcgctat
     5101 taattaacct cggcttaggt taccccttca gtttgctcta caccgctgcg tttacggcta
     5161 ttttgctttt gctatggcga acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt
     5221 cgctggtggc ggcttgttat ttcccttttg ctcaggccta cggcgcgcct aatttcaata
     5281 cattgctggc attgcactcc accaatatgg aagagtcgac cgaaatcctg acgatttttc
     5341 cgtggtacag ctacatggtc ggcatattta tttttgtgct cggcgtaata gcaatcaggc
     5401 gaaaaaaaga gagtgaaaaa gcgcgctgga ataccttcga cagcctgtgt ctggtattca
     5461 gtgtggcgac attttttgtt gctcccgtgc aaaacctggc ctggggcggc gtatttaaac
     5521 tgaaagatac tggctatccg gtatttcgtt ttgctaagga tgtcatcgtc aataataacg
     5581 aggtgattga agagcaagaa cggatggcaa aactttccgg aatgaaagat acctggacgg
     5641 tcactgccgt taagccgaag tatcagacct atgtggtggt gatcggtgaa agcgcgcgtc
     5701 gcgatgccct cggtgccttt ggcggtcact gggacaatac cccgtttgcc agcagcgtta
     5761 acggtttgat atttgctgac tacattgccg ccagtggctc cacgcagaaa tcgcttggct
     5821 taacgctcaa tcgcgtagtc gatggcaaac cacagtttca ggataacttt gtcaccctgg
     5881 caaatcgcgc gggcttccag acctggtggt tttccaacca gggtcaaatc ggcgaatacg
     5941 ataccgctat cgccagcatc gccaaacgag cagatgaagt gtacttcctg aaagaaggta
     6001 attttgaagc agataaaaat accaaagacg aagcgttact ggatatgacc gctcaagtgc
     6061 tggcgcaaga gcactcgcaa ccgcagctga ttgttctaca tctgatgggc tcacatccgc
     6121 aggcctgcga caggacacaa ggcaaatacg aaacctttgt gcagtcgaaa gaaacgtcgt
     6181 gctatctcta taccatgacg caaacggacg atttactgcg caagctgtac gatcagttac
     6241 gcaacagcgg cagcagcttc tcgctggttt acttttctga ccacggtctg gcctttaaag
     6301 agcgcggcaa agacgtgcaa taccttgccc atgatgataa gtatcagcaa aatttccagg
     6361 tgccttttat ggtcatttcc agcgacgata aagcgcatcg ggtgattaaa gcccgccgct
     6421 cagccaatga cttcttaggt tttttctccc agtggacggg aattaaagcg aaggaaatta
     6481 acatcaaata cccgtttata tctgagaaga aagccgggtc gatatacatc accaacttcc
     6541 agttacagaa ggtggattac aaccatctcg gaaccgatat tttcgacccg aagccttaaa
     6601 aacaaaaatc cgccccgaga ggcggatttt ttatatcacc aaagtgatta gaagcggtaa
     6661 ccaacaccgg caatccaggt gcctacgtca acgctacgaa tacggctctg ctcgtaagag
     6721 aagtccagag caacgttttc catcgggttg aactgcagac cagcaccgta ggagaaaccg
     6781 tagtcgctgg tgtcgtgttt gtaggtcggg tattcagtgg tctggaattt accataaccc
     6841 acacccacta caccgtagat gcttgcccag tcgttaatgc ggtaagccgg accagcagtg
     6901 atgccgtagt actggttttt gttgtagtca ccagagcttg cagtacggct tttctcggtg
     6961 taagtgaaag aaccgatcac acccagcggg ctgttgtctt cttcatagcg gtatttcagg
     7021 ttgaaaccgc ccattttgtt catttggccc tgagcgtcgc tctgtgcgta accgccagtt
     7081 acggtagaag tcgcagctac ggaagtacct gcggtgaaag ccagaactgc ggccagtgct
     7141 gaaagacatg caattttttt cataaccacc tcaaatgtga ttcaaataag tcctaagttt
     7201 taaatatatc aaaaattaat gggaaactct tcgcgatttg tgatgtctaa cgggccattt
     7261 catgtaacag aacgtttcca tacaccgcta tccatctaaa tttaaatcac tttttcagag
     7321 aactgcgtaa gtattacgca tgttttccct gtcattcatc cagattattc ctaatcacca
     7381 gactaataat tccatcaatc ctggcgcatt ttagtcaaaa cgggggaaaa ttttttcaac
     7441 aaatgctcaa ccagcattgg gtatatccag tacactccac gctttactta agtctagata
     7501 tttgtgggag aaaggatgcc tggttcatta cgtaaaatgc cggtctggtt accaataatc
     7561 atattgctcg ttgccatggc gtctattcag ggtggagcct cgttagctaa gtcacttttt
     7621 cctctggtgg gcgcaccggg tgtcactgcg ctgcgtctgg cattaggaac gctgatcctc
     7681 atcgcgttct ttaagccatg gcgactgcgc tttgccaaag agcaacggtt accgctgttg
     7741 ttttacggcg tttcgctggg tgggatgaat tatctttttt atctttctat tcagacagta
     7801 ccgctgggta ttgcggtggc gctggagttc accggaccac tggcggtggc gctgttctct
     7861 tctcgtcgcc cggtagattt cgtctgggtt gtgctggcgg ttcttggtct gtggttcctg
     7921 ctaccgctgg ggcaagacgt ttcccatgtc gatttaaccg gctgtgcgct ggcactgggg
     7981 gccggggctt gttgggctat ttacatttta agtgggcaac gcgcaggagc ggaacatggc
     8041 cctgcgacgg tggcaattgg ttcgttgatt gcagcgttaa ttttcgtgcc aattggagcg
     8101 cttcaggctg gtgaagcact ctggcactgg tcggttattc cattgggtct ggctgtcgct
     8161 attctctcga ccgctctgcc ttattcgctg gaaatgattg ccctcacccg tttgccaaca
     8221 cggacatttg gtacgctgat gagcatggaa ccggcgctgg ctgccgtttc cgggatgatt
     8281 ttcctcggag aaacactgac acccatacag ctgctggcgc tcggcgctat catcgccgct
     8341 tcaatggggt ctacgctgac agtacgcaaa gagagcaaaa taaaagaatt agacattaat
     8401 taaatttaca tttctgcatg gttatgcata accatgcaga atttctcgct acttttcctc
     8461 tacaccgtct ttatatatcg aattatgcaa aagcatattt attccgaaaa ttcctggcga
     8521 gcagataaat aagaattgtt cttatcaata tatctaactc attgaatctt tattagtttt
     8581 gtttttcacg cttgttacca ctattagtgt gataggaaca gccagaatag cggaacacat
     8641 agccggtgct atacttagtc tcgttaatta ctgggacata acatcaagag gatatgaaat
     8701 tatgagtacc gctaaattag ttaaatcaaa agcgaccaat ctgctttata cccgcaacga
     8761 tgtctccgac agcgagaaaa aagcaacagt agagttgctg aatcgccagg ttatccagtt
     8821 tattgatctt tctttgatta ccaaacaagc gcactggaac atgcgcggcg ctaacttcat
     8881 tgccgtacat gaaatgctgg atggcttccg caccgcactg atcgatcatc tggataccat
     8941 ggcagaacgt gcagtgcagc tgggcggtgt agctctgggg accactcaag ttatcaacag
     9001 caaaaccccg ctgaaaagtt acccgctgga catccacaac gttcaggatc acctgaaaga
     9061 actggctgac cgttacgcaa tcgtcgctaa tgacgtacgc aaagcgattg gcgaagcgaa
     9121 agatgacgac accgcagata tcctgaccgc cgcgtctcgc gacctggata aattcctgtg
     9181 gtttatcgag tctaacatcg aataaatcca tcgctgatgg tgcagaactt tagtacccga
     9241 taaaagcggc ttcctgacag gaggccgttt tgttttgcag cccacctcaa cgcacttatt
     9301 tagtgcatcc atctgctatc tccagctgat taagtaaatt ttttgtatcc acatcatcac
     9361 acaatcgtta cataaagatt gttttttcat caggttttac gctaaataat cactgtgttg
     9421 agtgcacaat tttagcgcac cagattggtg ccccagaatg gtgcatcttc agggtattgc
     9481 cctataaatc gtgcatcacg tttttgccgc atctcgaaaa atcaaggagt tgcaaaactg
     9541 gcacgatttt ttcatatatg tgaatgtcac gcaggggatc gtcccgtgga tagaaaaaag
     9601 gaaatgctat gaagtctgta ttaaaagttt cactggctgc actgaccctg gcttttgcgg
     9661 tttcttctca tgccgcggat aaaaaattag ttgtcgcgac ggataccgcc ttcgttccgt
     9721 ttgaatttaa acagggcgat aaatatgtgg gctttgacgt tgatctgtgg gctgccatcg
     9781 ctaaagagct gaagctggat tacgaactga agccgatgga tttcagtggg atcattccgg
     9841 cactgcaaac caaaaacgtc gatctggcgc tggcgggcat taccatcacc gacgagcgta
     9901 aaaaagcgat cgatttctct gacggctact acaaaagcgg cctgttagtg atggtgaaag
     9961 ctaacaataa cgatgtgaaa agcgtgaaag atctcgacgg gaaagtggtt gctgtgaaga
    10021 gcggtactgg ctccgttgat tacgcgaaag caaacatcaa aactaaagat ctgcgtcagt
    10081 tcccgaacat cgataacgcc tatatggaac tgggcaccaa ccgcgcagac gccgttctgc
    10141 acgatacgcc aaacattctg tacttcatca aaaccgccgg taacggtcag ttcaaagcgg
    10201 taggtgactc tctggaagcg cagcaatacg gtattgcgtt cccgaaaggt agcgacgagc
    10261 tgcgtgacaa agtcaacggc gcgttgaaaa ccctgcgcga gaacggaact tacaacgaaa
    10321 tctacaaaaa atggttcggt actgaaccga aataataacg ctacacctgt aaagcgcact
    10381 ggcagttccc tctcccctct ggggagagga ttagggtgag gggcgcaaac ccgctccggg
    10441 gccattaatt accctgaatt tgattattta caccacggta acaggaacaa catatgcagt
    10501 ttgactggag tgccatctgg cctgccattc cgcttctgat tgaaggtgcc aaaatgaccc
    10561 tgtggatttc ggtcctcggt ctggctggcg gtctggtaat cggattgctg gcaggttttg
    10621 cacgcacctt cggtggttgg atagccaacc acgtcgcgct ggtctttatt gaagtgatcc
    10681 gcggcacacc tatcgtcgtc caggtgatgt ttatctattt cgccctgccg atggcgttta
    10741 acgacttacg catcgaccca tttactgcgg cggtggtcac catcatgatc aactccggcg
    10801 cgtacattgc ggaaatcacg cgtggtgcgg tgctgtctat ccacaaaggt tttcgtgaag
    10861 caggactggc gctcggtctt tcacgttggg aaaccattcg ctacgtcatt ttaccgctgg
    10921 cactgcgtcg tatgctgccg ccgctgggta accagtggat catcagcatt aaagacacct
    10981 cgctgtttat tgtgatcggc gtggcggaac tgacccgtca ggggcaagaa attattgccg
    11041 gtaacttccg cgcccttgag atctggagcg ccgtggcggt gttctatctg attattaccc
    11101 tggtgctgag ctttattctg cgtcgtctgg aaagaaggat gaaaatcctg tgattgaatt
    11161 taaaaacgtc tccaagcact ttggcccaac ccaggtgctg cacaatatcg atttgaacat
    11221 tgcccagggc gaagtcgtgg tgattatcgg gccgtccggt tccggtaaat cgaccctgct
    11281 gcgctgcatc aacaaactgg aagaaatcac ctccggcgat ctgattgtcg atggcctgaa
    11341 ggttaacgat ccgaaagttg acgagcgcct gattcgccag gaagcaggta tggtgttcca
    11401 gcagttttac ctcttcccgc atctgacggc gctggaaaac gtcatgtttg gtccgctacg
    11461 cgtgcgtggc gcgaacaaag aagaggcgga aaaactggcg cgtgagctgc tggcgaaagt
    11521 cggtctggca gaacgtgcac atcactaccc ttccgaactt tctggtggtc aacagcagcg
    11581 tgtggcgatt gcccgcgcgc tggcggtgaa gccgaaaatg atgctgtttg atgaaccgac
    11641 ttccgctctt gacccggaac tgcgccatga agtgctgaag gttatgcagg atctggctga
    11701 agaagggatg acgatggtga tcgtgaccca cgaaatcggt tttgccgaga aagtagcttc
    11761 gcggcttatc tttatcgaca aaggccggat tgcggaagat ggcgatccgc aggtattgat
    11821 caagaacccg ccgagccagc gcttgcagga atttttgcag cacgtctctt aacttctctg
    11881 cctccctttc acccgaaagg gaggcacacc agattcctct catttaaaat cgcccctccc
    11941 ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc
    12001 ctgttcatcc tcttctgcct actgggcgca cctgcccacg cggtatccat acccggcgtt
    12061 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
    12121 aaaaaagcgg cctatggcgc actggcggat gtgctggata acgacacctc gcgtaaagaa
    12181 ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg cggaaccggt cccgaagatc
    12241 gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc
    12301 cattatggtg aatccctttc cgcccgcttc gggcaacttt atcgcaatat caccggctcc
    12361 ccgcataagc catttaatcc acaaaccttc agcaatgcgc tgagccattt ttcaatgtta
    12421 gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcactgcc gctgtatcgc
    12481 aaaatgggtc agtgggcgag gcaaaaaaat cgtgagcgca gcaactggtt gcagcttccg
    12541 gcgatgatta tcggggcgtt tattatcgac ctgctgttac tggccctgac attatttgtc
    12601 ggccaggtat taagcgacaa cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt
    12661 ttgtttctca acgcctttgc cctgattgaa tttttcaaag ccgtactacg cctgattttt
    12721 tgcccaaacg tggcggagct gcgcccgttc acgattcagg acgagagcgc ccgttactgg
    12781 agtcgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt ggccgtgccg
    12841 attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg
    12901 tgcatgaccg tctgggcgtt gtacctgatc tttcgtaata aaaaagagat tacccagcat
    12961 ttgctcaact ttgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg
    13021 ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc
    13081 gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt
    13141 ggtattgcag cgtttgtttc cggtatgttt tcccgctggc tggcaaaaac catcactctc
    13201 tcgccacata ctcagcgtaa ctatccagag ttgcaaaaac ggctgaatgg ctggctttcg
    13261 gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cggtaatgtt gctgttgagc
    13321 gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta
    13381 gatatcctga tccgtatcgc acttattctt tttttctcgg cggttggctg gacggtgctc
    13441 gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc
    13501 cgtacgcgta ccctgctgac gctgttccgt aacgcgctgg cggtgattat cagtaccatc
    13561 accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggggcc
    13621 ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga tatcattacc
    13681 ggggtgttta ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg
    13741 ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg
    13801 gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt ccgcggtatt
    13861 ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa agccaatcag
    13921 gcactgaaag atgcggtagc ggaattgatg gaaaacgaag aaattcgcgg gctgattatt
    13981 ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt
    14041 tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg
    14101 aaaaaacatt tcgacctggc gggcgttcgc gcgccagtgc agacttatca ggtgctgcct
    14161 gctccgggcg cgaccccggc tgaaccgccg ccgccggggg agccaacgct ttaacgttgg
    14221 cgattaacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc
    14281 tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcgcccac gtcggtcaga
    14341 gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa ccacatcacc
    14401 tgcttcgcga agcctttgct ctcttcaatc atctttttga taaagttgac ttcaccgcct
    14461 tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt gttcagcccc
    14521 aggttacggc gtttacgctc actacctgcc cgcgccgcag cggcggaatc gtggaatggc
    14581 gggttacaca aggtcgcgtc gtattgctcg tttttgtgga tgatgccgtt aaaaatcgcc
    14641 ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt agcactgata
    14701 atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa acgccagcca
    14761 tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg
    14821 ctggcattcg ctggaattgt tccgcttgct tcagccagta aatcggcaag gtgatgaata
    14881 taatccgccc ggcccggtac tggtgggcag agaaaaccgt cggggatatc ccagttcgct
    14941 acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca
    15001 aagtctacgc tttgctcccc tgcgggtgta agcgtgagga attgcctgag ttcaggattg
    15061 acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc
    15121 ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc cgcataacgc
    15181 gacagggtaa atatctggct gcgtttatca ttccccaaaa ccttaacatg gctatggttt
    15241 atggcaattc atcataaaaa tcagcattat gcatgtcatt gatttgtctt attttgtaaa
    15301 aatatatcgt tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt ttcatcgccc
    15361 aggcgaccta cacttatcag gcactacccg ttgtatttat gaggatgtca ttatgaaaaa
    15421 gtgcctcaca ctactgattg ccaccgtcct gagcggaatc tctctcacgg cttatgccgc
    15481 gcaaccgatg agtaacctgg acagcggtca gctacggccc gccggtaccg tttcggcaac
    15541 tggcgcatca aacctaagcg atctggagga taaactggca gaaaaagcgc gcgagcaagg
    15601 cgcgaaaggt tatgtcatta attccgcagg cggaaatgac cagatgttcg gtactgcaac
    15661 catctacaaa taaccccacc aacgtaactg cattcgcctc tggatgcagt tttcgcctgt
    15721 tttgtaatca aaaaacatca aatatgaacg caatgtaaat aaatgtattt ctttttcgcg
    15781 caatgggtga tagaaaatcg cgccaagtga taatgcttat caaaattatt atcacttgca
    15841 cgaacactat cacgagatta acagtggcat cgcatccgca gagaggcttt ctcgtggcag
    15901 tgaaaatttc aacatataaa aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct
    15961 gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggcat cacgcctgtg
    16021 gctcaggcac tcgccgccga agggcaaact aacgcggatg acacgctggt tgtcgaagca
    16081 tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta
    16141 gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca
    16201 accaacctta ccgacgcgct caaaaacgtc cccggcgtgg gtgcgttttt tgcgggtgag
    16261 aacggtaact ccaccactgg cgacgccatt tatatgcgcg gtgctgatac ctctaacagt
    16321 atttatattg atggcattcg cgatatcggc agcgtctcgc gcgacacctt caatactgag
    16381 caggtcgaag tgattaaagg gccttccggc accgactacg ggcgcagcgc gccgacgggc
    16441 tcgatcaata tgatcagcaa gcagccgcgc aatgattccg gcattgacgc ctccgccagt
    16501 attggcagcg cctggttccg ccgcggcacg ctggacgtca atcaggtcat tggtgatacc
    16561 actgcggtgc gcctgaatgt gatgggcgaa aaaacgcacg atgccggacg cgacaaagtc
    16621 aaaaatgagc gttacggcgt cgccccttct atcgcttttg gccttggtac agcgaatcgt
    16681 ttgtatctta attatctgca tgtcacccag cacaacacgc cagacggcgg cattccgacc
    16741 atcggtttgc cgggttattc tgccccatct gcgggaacag cggctctgaa tcattccgga
    16801 aaagttgata ctcataactt ttacggcacg gattccgatt acgacgattc gaccaccgac
    16861 accgccacca tgcgttttga gcacgacatc aacgataaca ccaccattcg caatactacc
    16921 cgttggtcgc gcgtaaagca ggattacctg atgacggcga ttatgggcgg ggcgtcgaat
    16981 attactcagc ccaccagcga tgtgaatagc tggacctggt cacgcacggc gaataccaaa
    17041 gatgtgagta ataaaattct caccaaccag accaacctga cctcgacgtt ctataccggt
    17101 tctatcggtc atgatgtcag taccggcgtg gaatttaccc gtgaaacgca gactaactac
    17161 ggcgttaatc cggtgacgtt acccgcggta aatatttatc atcctgacag cagcattcat
    17221 cccggcggcc tgacgcgcaa cggcgcaaac gccaatggtc agacggatac cttcgcaatt
    17281 tacgccttcg atacgctgca aatcacccgt gattttgagc tgaacggcgg gatccgtctg
    17341 gataattatc atactgaata tgacagtgcc accgcctgcg gcggcagcgg acgcggtgcc
    17401 atcacctgcc caactggtgt ggcaaaaggt tctccggtca ccaccgtcga caccgccaag
    17461 tcgggcaatc tgatgaactg gaaagccggg gcgctgtatc acctgacgga aaacggcaat
    17521 gtctatatta actatgccgt ttcccagcag cctccgggcg gcaacaactt cgcccttgcg
    17581 cagtctggca gcggtaacag tgccaaccgc accgatttta aaccgcaaaa agccaacacc
    17641 agcgagattg gcaccaaatg gcaggttctg gataaacgtc tgttgctcac cgccgcgctg
    17701 ttccgtactg atatcgaaaa tgaagttgag caaaatgatg acggaactta ctcgcaatac
    17761 ggtaagaaac gcgtcgaagg ctatgagata tccgtggccg ggaatatcac tcccgcgtgg
    17821 caggtgattg gcggctatac ccagcaaaaa gcaaccatca aaaacggcaa agatgttgcc
    17881 caggatggtt cctcatcgct gccgtatacc ccggagcacg ccttcacctt atggagccaa
    17941 tatcaggcaa ccgacgatat ctctgttggc gcgggcgcac gctatatcgg cagtatgcat
    18001 aaaggttcag acggcgcggt gggaacgcca gcatttaccg aaggttactg ggtcgccgat
    18061 gccaaactgg ggtatcgggt taatcgcaat ctcgacttcc agctaaacgt ctacaacctg
    18121 tttgataccg attacgtcgc ctcaatcaac aagagcggct accgttatca cccgggcgag
    18181 ccaagaacct tcttgctcac agccaatatg catttctgat tcagatgtgg ggcgcaggcc
    18241 ccactttttg gagaaattgt atgatgtacc acattcccgg cgtgttatcg ccacaggacg
    18301 tcgctcgttt tcgcgaacaa ctggaacaag ccgaatgggt ggatggacgc gtcaccaccg
    18361 gcgcacaagg tgcgcaagtt aagaacaatc aacaggtcga cacctgcagc acgttatacg
    18421 ccgccctgca aaatgaggtg ctgaacgcgg ttaaccaaca tgctttattc tttgccgcgg
    18481 ccttgccgcg taccctttcc acgccgctgt ttaatcgcta tcagaacaat gaaacctatg
    18541 gtttccatgt ggatggcgca gtacgcagcc atccacaaaa cggctggatg cgtactgacc
    18601 tttctgccac gctgttttta agcgatccac aaagctacga cggcggcgaa ctggtcgtta
    18661 atgacacctt cggacaacat cgggtaaaac tcccggcagg cgatctcgtg ttgtatccct
    18721 ccagcagcct gcattgcgtg acacccgtaa cccgcggcgt acgagtggca tcatttatgt
    18781 ggatccagtc gatgatccgc gatgataaaa agcgcgccat gctgtttgaa ctggacaaca
    18841 atattcagtc gctgaaaagc cgctacggcg aaagtgaaga gatcctgtcg ctgcttaatc
    18901 tttatcataa tctgctgcgg gaatggtcgg agatctgatg cttgaactgc ccgagttcgt
    18961 ccctggatta gctacactta actgtacaag tattgatatg gggaggtcga tatggcatcc
    19021 ggttgagcta acgatgacgc cgtcaacgaa cagatcaaca gtacaattga agatgcgatt
    19081 gcccgcgctc ggggtgaaat tccgcgcggc gaaagcctgg atgaatgtga agagtgcggt
    19141 gcccccatcc cacaggcccg tcgggaagcc attcctggcg tgcgcttatg tattcattgt
    19201 cagcaggaga aagatttaca aaaaccagct tatacaggat ataatcgcag aggttcgaaa
    19261 gacagccagt tacgttaact acaactggcg ggatgtatag cgagtataat ttgtattttg
    19321 cgtggcattc cgtgccttta acgctgtcgt gactgccacg cggtataaaa caagttcata
    19381 agtacaacaa ataaatggtt tatcagtatg ttagatatca atcaatttat ttgaacaagg
    19441 cggtcaattc tcttcgattt tatctctcgt aaaaaaacgt gatactcatc acatcgacga
    19501 aacaacgtca cttatacaaa aatcacctgc gagagattaa ttatgaaaac tatcaatact
    19561 gttgttgctg ctatggctct ttcaactctg tcatttggcg tgtttgccgc ggaaccggta
    19621 acggcatccc aggcacagaa catgaataaa atcggcgtgg tttctgccga tggcgcatcc
    19681 accctcgatg ccctggaagc gaaactggct gagaaagcgg cggcagccgg tgccagtgga
    19741 tacagcatca cttccgccac caacaacaat aaattaagcg gtactgcggt aatttataag
    19801 taattcgtag gacggataag gcgtttacgc cgcatccggc aacagatgcc tgatgcgacg
    19861 ctaccgcgtc ttatcaggcc tacaaaatcc gaaccgttgg acggatcagg cgtttacgct
    19921 acgtccagca caagtatttc cctgtctgtt tgccgacaga cgcatatgct ctaaccctca
    19981 ttgatcctat attacccttg tttgcccgtc cgttactgga cgggcttttt ttagctggct
    20041 aactgctgac agaaaccctg caacgtctct tccgacatac caatctgccg cgccgcatca
    20101 caaatggcct gccagcttcc cgcatccaat ggaatcccct gctcctgccg ttcgcgacgc
    20161 gtgttcactt cccactcgcc cggtagcaaa atcggcttat catcatcatg cggcgaggct
    20221 ttcacccact cggcaaaggc ttcggtctgc gcgctacaat ccggcgcgcc gaagagttcc
    20281 gggttgatga tgatagtggt catgcagtta agaatggcat cgggactggt ttgtaacgtt
    20341 tcctgatgcg tcgttctacc gccggaaagc gccccgccaa gaatttcaca catcgctgca
    20401 agggcgtagc ctttatgttc ggcaaaggtc aacagcgaac ccaacggcga ctcctgcatt
    20461 accgccggat tggtcgtcgg cacgccgtta acgtcaatca ggcaacctgg cggcacgggg
    20521 acgcctttat gccaggcgac gcgggttttg ccaaatgcaa tggcgctggt ggcgtaatca
    20581 agcaacagcg ggaaattatc tttacgaggg aaaaccacac agaagggatt ggtgccaaag
    20641 cggctgtcgc gaccgtggaa cggcgcgacc attggaatac cgaccacgct aacaaagtgg
    20701 atagagacaa accccgccgc tgcacactgc tccgcccagt aaccgatacg gccgatatga
    20761 tgcgagttat gtagcgccac ggcggcaata ccgtgctgat gcgctttctc aatccccagc
    20821 gccatcgctt catgtgccgc gacctgacca aatgcgcgat cgccatcgag cgtgaccgcc
    20881 gcccccgact ctttaacggt tttggcatga tggttaattt gcaggtgccc ctgactccag
    20941 gagcgtacat agcttgggat catgccaata ccatgtgaat catgccctgc caggtttgcc
    21001 gcgattaaat gatcggcaac taatttcgct tcttgttcct cgctacccat ctgacgaaat
    21061 acagcctgaa taaaactgtg cagcgtctga gcatcaaagc gatgaccact ttccatggtt
    21121 aactcctgtt tatgttatgt gtttgttgta tttttatgct gcacgccggg cgtgggaagg
    21181 tcaataaaga tgcaggaatt tcatattgcg gtggggtaaa agccggatga catggctcat
    21241 ccggctgaga aaagaattaa aatgcctgat taacgcgcgc caggccgtcg ctgatagttg
    21301 ccgcttcacc cgtagccacc aggcagcaag ccatctggat tttcagcgat tcgggaatcg
    21361 gttcgctgcc agcaaggcaa cgctcaatcc actgcgccgt ggtttccgga tcttttgctt
    21421 gtggcagtaa ctcgctacca gcagtgtcct gtttttcata cagcacccgc atcccttcac
    21481 ggtcaatgag attgatctgc gggcagcgct gcggattagc atacacttca ccttcagtac
    21541 catgcattag tagcgcccgc ccgccgatat cgctaaagaa cttcgcgacg cgtccaatgt
    21601 attccggatg cgaaacgctg gaaagacgca gcgcctcacc ttcggcaaat ggcgttgcca
    21661 gtttcgccag ggtatgtgca ctgttacgca cgcccatccg ccagcgcatc gccagttgtt
    21721 tttccagcgg cgggcaaaac gcgccgactg gcataaacac cggttgatgt tcgtcgagct
    21781 tcgcctgcgc ctgcccgccg tgcaacgttg gcgtgatacc catcaattca aaaatggttt
    21841 cagtcagcac gcgggttgga tcttcgctaa ccccgtgaac caccacagga aaaccgagtt
    21901 tgtgcaggag aatcgccaac aacggcgtca ggttggcctg tttacgcgcg ccgttgtaac
    21961 tggggatgac aatcggcatc ggcttgcctg caggcggcgt cagcttgatg gtgtgatttt
    22021 gtatggcttc gtaaaagccg agcatctctg cttccccttc ccctttgata cgcagcgcaa
    22081 tcaatacgcc gcccaactca aggtcaggga cttcaccatt gagcatatgg gcatacagac
    22141 cgcgcgcagt atcccggtct aaatcgcgcg cgtggttttt cccgcgcccg atctctttaa
    22201 tgattttgcg atagtccatt tacgactcct tacctgactc acatcattaa cgccgccgac
    22261 ggcgtgggga tttcgttttt tctttctttt taactatacc ttcagggact tccggttgct
    22321 ctatcggaaa taccggtaat gcatccagta gtcgcttgcc ataatttttg gtcagcaagc
    22381 gtttgtcgta gataaccact tcgccccagc aaccgtggct tcgaatcagt cgcccaacct
    22441 gctgaatcag gttaaacgag gcgctcggca ggctttgcac ctcaaacgga tagcggttga
    22501 ggcttttcag ccattcccct tcggtgatca ccaccgggct gtcgatgggc ggaaaagcga
    22561 ttttgtggat atgcacctgg ctgagcagat cacctttcaa atcaagccct tcggcaaatg
    22621 actgtaagcc caccagcaca ctacgctcac cgttggcaac gcgtttgcgg tgcagttcaa
    22681 ctaagcgata acgcggctga tcgccctgca ccagcagcat caggcgtaaa tccgtcacat
    22741 agtcgagaaa gcgctgcatc gcccgtccgc tggcaaacag taccaacata ccgagatgtt
    22801 ttttgctctc cacctgctta cggaaaaagg ccgccatttc ggcaatatgc tgctcttcgt
    22861 tgtcgatgga aggctcaaag cgcatccggg gaataacaat tttgccctgt tcgcagtggt
    22921 taaaggggga atccagcgcc acaaaacggt cgcccgcttt ctctttcaga ccactcatct
    22981 cctgcaaacg cgaaaaactg ttcagcgaac gcaaggttgc ggaggtgaca ataatgtgcg
    23041 gaatactgcg ccacagcagc ctttccagct gatcgctgac acgtattccc acgcagtgaa
    23101 accagagatg cagctgccct tcgcgctctt cccgcgtcgc ccatttggtc accggtgcac
    23161 cggaagattg cgccagagac gccagccgcc agagtttgct ttgcgcctcg aacatcccca
    23221 acgcgcggtt catctgcaaa atcaaccgat gcaggcgtac aatgtcatgg ctgccggttt
    23281 tctcacttaa atcgttaaga aataactccg ccagaccacg cagcatctcg gtgagttttg
    23341 ccagccgctg gcagatctcc agcacttcat ctggcagttc gcccatcgca aaacggtgct
    23401 ctgcctcctg cccggcaggc atgtagagat cgagaatgtt gtttaacgag gcgataagct
    23461 catacaactc ttcacaatgc gcattcaaac gttcagggat cgccagcggc gggatggtct
    23521 tcgggcgaaa ctgctccatg caggtagcga ccagtttcgt gaacaagtcc agctgtagtc
    23581 gataccaggg tgcggtgatt tcggcgctca tctccagcgc atcccgcgcc acatccggca
    23641 ggtgatgacc ttcgtccagc accagcagta aatttttcgg gtcaggcaat accgcttcgc
    23701 tttccatcgc cgccatcacc agcgcatggt ttgccaccac cacttccgct tcctgaatct
    23761 cccgacgagc aacaaaaaac gggcattcac gatagtagta acagttacgg ttgaggcagc
    23821 tggctttgtc ggtactcaaa cgacgccaga gatcgtcatt tatagcgata tccgtatgat
    23881 cgcgcagacc atcccattta taagtatcga gatcgccctt cagcttcgca caacgttttt
    23941 gctcttcctg attgttcggc gtcagttcgt cgtcaagaaa cgccagcaga tcctgttgcg
    24001 tgggttcagt actggcgagc gccgtcagat tacgcggaca aacgtagcgc ccacgcccaa
    24061 aagcggcagt gaatttaaga tcgggaatga tctttttcag cagcggtaaa tctttgctgt
    24121 aaatctgatc ctgcaatgcc acgttggcgg tactcaccac cagcgttttt tgctcttcgc
    24181 gggcaatggc gatgccggga atcaaataag agagcgtttt cccaacgccg gtgggggctt
    24241 caatcgccag atgtcgccct tcttctccgg ccagcgtttt ggcgacgtcc gcaatcatct
    24301 gccgctgcgg cgcacgggga ataaagtcgg ggatctgttc ctgaagcgcc ttataccagg
    24361 cggcaatttg cgctttaagc gcggcggtta atgccatgag aaaacctgaa atactgtata
    24421 aacagccaat attgtggcat ttttcggttt tcaggggtaa tggtttttct gtttctggaa
    24481 agcggctcgc aataaccacc aacataatgc ggtttaatgc ccgttcgagc acaaaggatc
    24541 caacacgtca tgcaagacac catcataaat ttctacagca ccagcgatga ctacggtgat
    24601 ttttctaact ttgccgcctg gcccatcaag gttgacggga aaacctggcc cacctcagaa
    24661 cactattttc aggcacaaaa attccttgat gaaaaatacc gcgaagagat ccgtcgggtt
    24721 tcttccccta tggtcgccgc acgcatgggg cgcgatcgtt ctaagcctct gcgtaaaaac
    24781 tgggagtcgg tcaaagaaca ggtgatgcga aaagcacttc gcgctaaatt cgaacagcat
    24841 gcagaactgc gtgcgctctt gctggcaacc gcgcccgcaa aactggttga gcatacggaa
    24901 aacgatgctt actggggaga cggtggtaat ggtaagggca agaatcgact gggctacctt
    24961 ttaatggagt tgcgcgaaca attggctata gagaagtaac gtctcggtgg cttatgggcg
    25021 gtaaaacaga taaatttttt cccgtgttct caaattgctg acaagtgctt gttgtgaaat
    25081 gccggatgcg gcgtaaacgc cttatccggc ctacaaaagt atgcaaattc aatatattgc
    25141 agagtt
//
LOCUS       DAZKIV010000060        25074 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZKIV010000060 DAZKIV010000000
VERSION     DAZKIV010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25074
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.060"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(58..306)
                     /locus_tag="ACHOT6_002365"
     CDS             complement(58..306)
                     /locus_tag="ACHOT6_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9947570.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            526..2112
                     /gene="prfC"
                     /locus_tag="ACHOT6_002366"
     CDS             526..2112
                     /gene="prfC"
                     /locus_tag="ACHOT6_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFP9947571.1"
                     /translation="MTSLLAAEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAGT
                     VKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRTL
                     TAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVENE
                     LKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAAV
                     GEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWAP
                     APMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQVR
                     TAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPNF
                     APELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVVS
                     RLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSMV
                     NLRLAQERYPDVQFHQTREH"
     gene            2505..3110
                     /gene="osmY"
                     /locus_tag="ACHOT6_002367"
     CDS             2505..3110
                     /gene="osmY"
                     /locus_tag="ACHOT6_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFP9947572.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            3237..3398
                     /locus_tag="ACHOT6_002368"
     CDS             3237..3398
                     /locus_tag="ACHOT6_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFP9947573.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            3520..4593
                     /locus_tag="ACHOT6_002369"
     CDS             3520..4593
                     /locus_tag="ACHOT6_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFP9947574.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            4590..5372
                     /locus_tag="ACHOT6_002370"
     CDS             4590..5372
                     /locus_tag="ACHOT6_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9947575.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAARVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(5485..6348)
                     /locus_tag="ACHOT6_002371"
     CDS             complement(5485..6348)
                     /locus_tag="ACHOT6_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFP9947576.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHIRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(6320..7870)
                     /locus_tag="ACHOT6_002372"
     CDS             complement(6320..7870)
                     /locus_tag="ACHOT6_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFP9947577.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADADLKQVSPNLTFIYDPEITPDDLLLKVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            8128..8907
                     /gene="deoC"
                     /locus_tag="ACHOT6_002373"
     CDS             8128..8907
                     /gene="deoC"
                     /locus_tag="ACHOT6_002373"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFP9947578.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            9034..10356
                     /gene="deoA"
                     /locus_tag="ACHOT6_002374"
     CDS             9034..10356
                     /gene="deoA"
                     /locus_tag="ACHOT6_002374"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFP9947579.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            10408..11631
                     /gene="deoB"
                     /locus_tag="ACHOT6_002375"
     CDS             10408..11631
                     /gene="deoB"
                     /locus_tag="ACHOT6_002375"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFP9947580.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            11711..12430
                     /gene="deoD"
                     /locus_tag="ACHOT6_002376"
     CDS             11711..12430
                     /gene="deoD"
                     /locus_tag="ACHOT6_002376"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFP9947581.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(12591..12854)
                     /locus_tag="ACHOT6_002377"
                     /pseudo
     CDS             complement(12591..12854)
                     /locus_tag="ACHOT6_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(12886..13902)
                     /gene="lplA"
                     /locus_tag="ACHOT6_002378"
     CDS             complement(12886..13902)
                     /gene="lplA"
                     /locus_tag="ACHOT6_002378"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFP9947582.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            complement(13930..14574)
                     /locus_tag="ACHOT6_002379"
     CDS             complement(13930..14574)
                     /locus_tag="ACHOT6_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFP9947583.1"
                     /translation="MARTKLKFQLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            14680..15648
                     /gene="serB"
                     /locus_tag="ACHOT6_002380"
     CDS             14680..15648
                     /gene="serB"
                     /locus_tag="ACHOT6_002380"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFP9947584.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15697..17079
                     /gene="radA"
                     /locus_tag="ACHOT6_002381"
     CDS             15697..17079
                     /gene="radA"
                     /locus_tag="ACHOT6_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFP9947585.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            17100..18332
                     /gene="nadR"
                     /locus_tag="ACHOT6_002382"
     CDS             17100..18332
                     /gene="nadR"
                     /locus_tag="ACHOT6_002382"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFP9947586.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(18639..20306)
                     /gene="ettA"
                     /locus_tag="ACHOT6_002383"
     CDS             complement(18639..20306)
                     /gene="ettA"
                     /locus_tag="ACHOT6_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFP9947587.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            20517..22454
                     /gene="sltY"
                     /locus_tag="ACHOT6_002384"
     CDS             20517..22454
                     /gene="sltY"
                     /locus_tag="ACHOT6_002384"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFP9947588.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     MKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            22544..22870
                     /gene="trpR"
                     /locus_tag="ACHOT6_002385"
     CDS             22544..22870
                     /gene="trpR"
                     /locus_tag="ACHOT6_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFP9947589.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(22912..23424)
                     /gene="yjjX"
                     /locus_tag="ACHOT6_002386"
     CDS             complement(22912..23424)
                     /gene="yjjX"
                     /locus_tag="ACHOT6_002386"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFP9947590.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVY"
     gene            23476..24123
                     /gene="gpmB"
                     /locus_tag="ACHOT6_002387"
     CDS             23476..24123
                     /gene="gpmB"
                     /locus_tag="ACHOT6_002387"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFP9947591.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            complement(24120..24989)
                     /gene="robA"
                     /locus_tag="ACHOT6_002388"
     CDS             complement(24120..24989)
                     /gene="robA"
                     /locus_tag="ACHOT6_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFP9947592.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
BASE COUNT         5898 a         6454 c         6863 g         5859 t
ORIGIN      
        1 acttccgata gtgcgagtat tgaatgattt ccagccgtta ccgattttac gtgttaatta
       61 gtgaacaaac cactcgtcag cagactccca ggtatctttc agagtttcct gaacaaaagt
      121 tttagctgaa tctttatcgg cggtgcgcgt aacagaaagg ccatcgttgc tggtggcttt
      181 tacgatcacc tctacatcgt cataacgctt actgatgcgt cgagttaatt cttcctttaa
      241 cgcatccaca gcaccgtttg gcattttagt cattttctct ttggctatgc agatctcaat
      301 acgcataaaa atccctctac actgtgtttg tatacagtgt tatttttaac tgtatgaata
      361 aacagtgtca atagaacaaa gattgctgca tgtacaaatg ttctttggct acaaatacac
      421 ctactgatca tgttctgcta tgctctgata cctccaaaat gtacaatcag cggcatgaat
      481 ttttctttgt acatatgatt gtacataatc aaagagacaa caattatgac aagccttttg
      541 gccgcagagg tagcgaaaag aagaaccttt gcaattattt ctcacccgga tgccggtaag
      601 actaccatca ccgagaaggt gctgctgttc ggacaggcca ttcagaccgc cggtacagta
      661 aaaggccgtg gttccaacca gcacgctaag tcggactgga tggagatgga aaagcagcgt
      721 gggatctcca ttactacgtc tgtgatgcag tttccgtatc acgattgcct ggttaacctg
      781 ctcgacaccc cggggcacga agacttctcg gaagatacct atcgtaccct gacggcggtg
      841 gactgctgcc tgatggttat cgacgctgca aaaggtgttg aagatcgtac ccgtaagctg
      901 atggaagtta cccgtctgcg cgacacgccg atcctcacct ttatgaacaa acttgaccgt
      961 gatatccgcg acccgatgga actgctcgat gaagttgaga acgagctgaa aatcggctgc
     1021 gcaccgatca cctggccgat tggctgcggc aagctgttta aaggcgttta ccacctttat
     1081 aaagacgaaa cctatctcta tcagagcggt aaaggccaca cgattcagga agtccgtatt
     1141 gttaaagggc tgaataaccc ggatctcgac gctgcggttg gtgaagatct ggcacagcag
     1201 ctacgtgacg aactggaact ggtgaaaggc gcgtctaacg agttcgacaa agaattgttc
     1261 cttgcgggcg aaatcactcc ggtattcttc ggtactgcgc tgggtaactt cggcgttgat
     1321 catatgctgg atggcctggt ggagtgggcc cctgcgccga tgccgcgtca gactgatacc
     1381 cgtaccgtag aggcgagcga agacaaattt accggcttcg tatttaaaat tcaggccaac
     1441 atggacccga aacaccgcga ccgcgtggcg tttatgcgcg tagtatccgg taaatatgaa
     1501 aaaggcatga agctgcgcca ggtgcgtact gcgaaagatg tggttatctc cgacgcgctg
     1561 acctttatgg cgggcgaccg ttcgcacgtt gaagaagcgt atccgggcga tatcctcggc
     1621 ctgcacaacc acggcactat tcagatcggc gacaccttta cccagggtga gatgatgaag
     1681 ttcaccggta ttccgaactt cgcaccagaa ctgttccgtc gtatccgcct gaaagatccg
     1741 ctgaagcaaa aacagctgct caaagggctg gtacagcttt ccgaagaggg cgcggtgcag
     1801 gtgttccgtc cgatctccaa caacgatttg atcgttggtg ctgttggtgt gctgcagttt
     1861 gatgtggtgg tatcgcgcct gaagagcgaa tacaacgttg aagcagtgta tgagtcagtc
     1921 aacgttgcca ctgcccgctg ggtagaatgt gcggacgcga agaaatttga agagttcaag
     1981 cgtaagaacg aaagccaact ggcgcttgat ggcggcgata atctcgctta catcgctacc
     2041 agcatggtta acctgcgcct ggcacaggaa cgttatccgg acgttcagtt ccaccagacc
     2101 cgcgagcatt aattcttgcc tccagggcgc ggcaaccgcc tcgccctgtc aatttccctt
     2161 ccttattagc cgcttacgga atgttcttaa aacattcact tttgcttatg ttttcgctga
     2221 tatcccgagc ggtttcaaaa ttgtgatcta tatttaacaa agtaatgaca tttctgacgg
     2281 cgttaaatac cgttcaatgc gtagatatca gtatctaaag ccgtcgattg tcattctacc
     2341 gatattaata actgattcag aggctgtaat ggtcgttatt catcacccat cgcttttgtg
     2401 atggcgacca ttgacttctg tagagggtga agtctctccc tattcagcaa tgcaacctcg
     2461 tgttgccagg ctcaaattac gagcaaacat acaggaataa atcgatgact atgacaagac
     2521 tgaagatttc gaaaactctg ctggctgtaa tgttgacctc tgccgtcgcg accggctctg
     2581 cctacgcgga aaacaacgca cagactacca atgaaagcgc agggcaaaaa gtcgatagct
     2641 ctatgaataa agtcggtaat ttcatggatg acagcgccat caccgcgaaa gtgaaggcgg
     2701 cgctggtgga tcatgacaac atcaagagca ccgatatctc tgtcaaaacc gatcaaaaag
     2761 tcgtgaccct gagcggtttc gttgaaagcc aggctcaggc cgaagaggca gtgaaagtgg
     2821 cgaaaggcgt tgaaggggtg acctctgtca gcgacaaact gcacgttcgc gacgctaaag
     2881 aaggctctgt gaagggctac gcgggtgaca ccgccaccac cagtgaaatc aaagccaaac
     2941 tgctggcgga cgatatcgtc ccttcccgtc atgtgaaagt tgaaaccacc gacggcgtgg
     3001 ttcagctctc cggtaccgtc gattcccagg cacagagcga ccgtgctgaa agtatcgcca
     3061 aagcggtaga tggtgtgaaa agcgttaaaa atgatctgaa aactaagtaa ttcgtcgtaa
     3121 ttcgtcctcc cgaactttgt cgggaggcgc gatgtgcacc acactaaaaa tatcgcattg
     3181 agtgattttc acgctcatat caagcggtga cattaactat ggtaaaggag acgcttatgt
     3241 ttcgttgggg catcatattt ctggttatcg cgttaatcgc cgccgcactt gggtttggtg
     3301 gtctggccgg taccgctgca ggcgcagcta aaattgtctt tgtcgtcggg attattctgt
     3361 tcctggtgag tttgttcatg ggccgaaaac gaccctagat ttcagaccat taagaaaatt
     3421 ttatccaaag ccagtccagc ggactggctt ttgcggtttt agcgaataat aaattgcgtt
     3481 actttgtcat tctttcacaa caaaaacagg aaggcagagg tggggcagcg aatacctgta
     3541 acgcttggta atattgcgcc gttgtcgcta aggccgttcc agcctggacg aatagctctg
     3601 gtgtgcgaag gcggcggaca gcgtggaatt ttcacggctg gcgtgctgga tgagtttatg
     3661 cgcgcgcagt ttaatccttt cgatctttat ctcggcacat ctgccggggc gcagaacctc
     3721 tcggcgttta tctgcaatca gcccggttac gcgcgcaaag tcatcatgcg ctataccaca
     3781 aaacgcgaat ttttcgatcc attgcgcttt gtccgtggag gaaatcttat cgatctcgac
     3841 tggctggtgg aggccaccgc aagccagatg ccgttgcaaa tggataccgc cgcgcggttg
     3901 tttgacagcg gcaaatcgtt ttatatgtgc gcctgtcgtc aggatgacta cgcgcctaat
     3961 tactttttac caaccaaaca aaactggctg gatgtgattc gcgcctccag tgcgatacct
     4021 ggcttttatc gtagcggagt gtcgctggaa ggcattaact acctggatgg cgggatcagt
     4081 gatgcgattc cggttaaaga ggcggcaagg cagggcgcta aaacgttggt cgtcatccgc
     4141 actgtgccgt cacaaatgta ctacacgccg cagtggttca aacgcatgga acgctggctg
     4201 ggtgacagta gcctgcagcc gctggtcaat ctggtgcagc atcatgaaac cagctatcgt
     4261 gacattcagc aatttattga gaaaccaccg ggcaagctgc ggatattcga aatttatcca
     4321 ccgaagccat tacatagtat cgcgcttggc agtcggattc cggcgctgcg tgaagactat
     4381 aaacttgggc gtttatgcgg tcgttatttc ctcgccacgg ttggcaagct attaactgaa
     4441 aaagcgccgc ttacccgcca tctggtgcca gtggtgacgc cggaatcgat cgttattccg
     4501 cctgcgccag tcgccaacga tacgctggtt gccgaagtga gcgatgctcc gcaggcgaac
     4561 gacccgacat ttaacaatga ggatctggct tgatttgccg ttttatcgat acccactgcc
     4621 attttgattt cccgccgttt agtggcgatg aagaggccag cctgcaacgc gcggcacaag
     4681 cgggcgtagg caagatcatt gttccggcga cagaggcgga aaattttgcc cgcgtgcagg
     4741 cgttagcgga aaaatatcaa ccgctttatg ccgcattggg gttgcatccg gggatgttgg
     4801 aaaaacatag cgatgtgtct cttgatcagc tacagcaggc gctggaaagg cgtccggcga
     4861 aggtggtggc ggtgggggag atcggtctgg atctcttcgg cgacgatccg caatttgaga
     4921 ggcaacagtg gttactcgac gaacagctga aactggcgaa acgctacgat ctgccggtga
     4981 tcctgcattc acggcgcaca cacgacaaac tggcgatgca tcttaaacgc cacgatttat
     5041 cgcgcactgg cgtggttcac ggtttttccg gcagcctgca acaggccgag cggtttgtac
     5101 agctgggcta caaaatcggc gtaggcggta ctatcaccta tccacgcgcc agtaaaaccc
     5161 gcgatgtcat cgcaaaatta ccgctggcat cgttgttgct ggaaaccgac gcgccggata
     5221 tgccgctcaa cggctttcag gggaagccta accgcccgga acaggctgcg cgggtgtttg
     5281 acgtgctttg cgagttacgt ccggaaccag aagatgagat tgccgaagtg ttgcttaata
     5341 atacgtatgc ggtgtttagc gttcgtgggt agtttaccct tcgtaggcag catccggcag
     5401 tcggcgcata atgcctgatg cgacgcttgt cgcgtcttat catgcctaca ggtttgtgcc
     5461 gaaccgtagg ccgtatccgg catgtcacaa atagagcgcc ggaaatatca accggctcac
     5521 cccgcgcacc tttaacgcat cagccaacgg ctcaacgtct tccggcgtgg cgctcgccca
     5581 gctttgcgcc tcgccataca cgccgtgggc atgaaacgca ttcaggcgaa ccggaacatc
     5641 gccaagtctc ttgataaacg tcgccagctc atcgatgtga tgcaaataat ccacctggtc
     5701 tggaattacc agcaaacgca gttccgccag cttaccgcgc tctgccagca aacagatgct
     5761 gcgcttaatc tgctgattat cgcgtccggt taattgctga tgacattcgc tcccccacgc
     5821 tttgagatcg agcatcgcgc cgtcgcacac cgggagtaat ttttcccagc cggtttcgct
     5881 caacataccg ttactgtcca cgaggcaggt gaggtggcgc agttgcggat cgtttttgat
     5941 ggctgtaaat agcgccacta caaacggcag ttgggtcgtg gcttcgccgc cgcttaccgt
     6001 tatcccttca ataaacagca ccgctttgcg gatatggcta agcacctcat ccacgctcat
     6061 ggattgcgcc attggcgtgg catgttgcgg acacctcttc aggcaggtat cacactgctc
     6121 gcaaaccaca gcgttccaca ccactttgcc gtcaacaatc tgcaacgcct gatgcggaca
     6181 ctgtggcacg cactccccac agtcattgca acgtcccatc gtccacggat tgtgacagtt
     6241 tttgcagcgc agattgcagc cctgcaaaaa cagagccaga cgactgcctg gcccgtcaac
     6301 gcaggagaag gggataatct tactgactaa agcgcatctg ctgttcatgg cttatcacgc
     6361 gcggctggcg ttccagaata cgagtgttgc gtgcggcttc ttcgcccagc caggtggtgt
     6421 tggtgcgtga accttcggcg cgatattttt ctaagtccga caaacgcacc atataaccgg
     6481 taacgcgaac cagatcgtta ccgctgacat tggcggtaaa ttcacgcatt ccggctttaa
     6541 aggcaccgag gcaaagctgt accagtgcct gcgggttacg tttgatggtt tcgtcgagcg
     6601 tcagaatgtc gctgatgccg gaataataat aagcatgatg cggggcgaca gtttgcagat
     6661 gggtgatcgg atctggctca tcgccatacg gcaaacgcgc gcccggcgtg gtgccgatat
     6721 cggaactgat ccccgactgt gcgtgtaaca tggcgcgttt ttgccagcca tatttcacgg
     6781 gggtattggc gacaaactcc gccagttgcg cgctgatgcg ataacccact tcatttgcgg
     6841 cggcttcttt accgtagcgc gcggcaatcc cttctttctc acacagcaag ttaaccgctt
     6901 ccgccagccc atacatgcca aacattggca caaaacgttc agggttaatc agcccttctt
     6961 tcaccaggaa gctattctca aagaagtgtg attgttgata gaggaattca caccgcgcat
     7021 cgatgatggc gatctgctgc tggcagtagt gcggtagagt gcgcgtaaag aagtcatcca
     7081 gcgattcgct gcgctcggca atggctttca ggttaaggcg taccagcgtg ctgccaccac
     7141 ccgccagcgg cagtgagttg taacagctca caatcccgta gccccctttt gtgaaaattt
     7201 tatcatgcac cggaccgttg gcgatgtgcg gtttgctaca ttcacagatg ttcttcgcca
     7261 ctttcagcag caggtcatca ggggtgattt caggatcgta gataaaggtc aggttcggtg
     7321 aaacctgctt caaatctgcg tctgcacgta agatcgcgcg ggtaatgggc gaatcagacg
     7381 ggccgatatt ggcgtgcata aaggcgtctg gcagggttct gtcgaggtaa cgccagaaac
     7441 gttttattcg aacatcgatc tcgtcttgtg ttagaattct aacatatggt tgcaacaacg
     7501 catccagttg ccccaggtag accggcatcg atgtgaccga tggtacgtgg tggtaaagaa
     7561 tggtcagcag agagagtgcg tcatcaagat ctttcgcgcc ttccagctcc agccattcgg
     7621 aaccgttcgc cagaaaacgg gcgtaatcgg gtaagacata gcgcggtttg tacggcgcat
     7681 gaccttcaaa catatcgcag attacaccct catccagcgc gcggcgggct tcggcaggaa
     7741 gctgcggata aggcagattg ttttctgctt ccagagccag aaaatggcgc ttctgctccg
     7801 ggctaagcac tgggctggtg acaatttgct ggcaacgttg ttgcagtgca ttttcatgag
     7861 aagtgggcat cttcttttcc ttttatgccg aaggtgatgc gccattgtaa gaagtttcgt
     7921 gatgttcact ttgatcctga tgcgtttgcc accactgacg cattcatttg aaagtgaatt
     7981 atttgaacca gatcgcatta cagtgatgca aacttgtaag tagatttcct taattgtgat
     8041 gtatatcgaa gtgtgttgcg gagtagatgt tagaatacta acaaactcgc aaggtgaatt
     8101 ttattggcga caagccagga gaatgaaatg actgatctga aagcaagcag cctgcgtgca
     8161 ctgaaattga tggacctgac caccctgaat gacgacgaca ccgacgagaa agtaattgct
     8221 ctgtgtcatc aggccaaaac tccggtcggc aataccgccg ctatctgtat ctatcctcgc
     8281 tttatcccga ttgctcgcaa aacgctgaaa gagcagggca ccccggaaat ccgaatcgct
     8341 acggtaacca acttcccaca cggtaacgat gacatcgaaa tcgcgctggc agaaacccgt
     8401 gcggcaatcg cctacggtgc tgatgaagtt gacgttgtgt tcccgtaccg cgcgctgatg
     8461 gcgggtaacg agcaggttgg ttttgacctg gtgaaagcct gtaaagaggc ttgcgcggca
     8521 gcgaatgtac tgctgaaagt gatcatcgaa accggcgaac tgaaagacga agcgctgatc
     8581 cgtaaagcgt ctgaaatctc catcaaagcg ggtgcggact tcatcaaaac ctctaccggt
     8641 aaagtggctg tgaacgcgac gccggaaagc gcgcgcatca tgatggaagt gatccgtgat
     8701 atgggcgtag aaaaaaccgt tggtttcaaa ccagcgggcg gcgtgcgtac tgcggaagat
     8761 gcgcagaaat atctcgccat cgcagatgaa ctgttcggtg ctgactgggc agatgcgcgt
     8821 cactaccgct ttggtgcttc cagcctgctg gcaagcctgt tgaaagcgct gggccacggt
     8881 gacggtaaga gcgccagcag ctactaagta agatgcttta cgcctgatgc gctgcgctta
     8941 tcaggcctac gagacgtatc tacccgtagg ccggataagg cgtagacgca tccggcaaaa
     9001 gccgcctcat actcttttcc tcgggaggtt accttgtttc tcgcacaaga aattattcgt
     9061 aaaaaacgtg atggtcatgc gctgagcgat gaagaaattc gtttctttat caacggcatt
     9121 cgcgacaaca ctatctccga agggcagatt gccgccctcg cgatgaccat tttcttccac
     9181 gatatgacaa tgcctgagcg tgtctcgctg accatggcga tgcgagattc aggaaccgtt
     9241 ctcgactgga aaagcctgca tctgaatggc ccgattgttg ataaacactc caccggcggc
     9301 gtcggcgatg tgacttcgct gatgttgggg ccgatggtcg cagcctgcgg cggctatatt
     9361 ccgatgatct ctggtcgcgg cctcggtcat actggcggta cgctcgacaa actggaatcc
     9421 atccctggct tcgacatttt cccggatgac aaccgtttcc gcgaaattat taaagacgtc
     9481 ggcgtggcga ttatcggcca gaccagctca ctggctccgg cagataaacg tttctacgcg
     9541 acccgcgata ttaccgcaac cgtggactcc atcccgctga tcactgcctc gatcctggcg
     9601 aagaaactgg cggaaggtct ggatgcgctg gtgatggacg tgaaagtggg tagcggcgcg
     9661 tttatgccga cctacgaact ctctgaagcc cttgccgaag cgattgttgg cgtggctaac
     9721 ggcgctggcg tgcgcaccac cgcgctgctc accgacatga atcaggtact ggcctccagt
     9781 gcaggtaacg cggttgaagt tcgtgaagcg gtgcagttcc tgacgggtga atatcgtaac
     9841 ccgcgtctgt ttgatgtcac gatggcgctg tgcgtggaga tgctgatctc cggcaaactg
     9901 gcgaaagatg acgccgaagc gcgcgcgaaa ttgcaggcgg tgctggacaa cggtaaagcg
     9961 gcagaagtct ttggtcgtat ggtagcggca caaaaaggcc cgaccgactt cgttgagaac
    10021 tacgcgaagt atctgccgac agcgatgctg acgaaagcag tctatgctga taccgaaggt
    10081 tttgtcagtg aaatggatac ccgcgcgctg gggatggcag tggttgcaat gggcggcgga
    10141 cgccgtcagg catctgacac catcgattac agcgtcggct ttactgatat ggcgcgtctg
    10201 ggcgaccagg tagacggtca gcgtccgctg gctgttatcc acgcgaaaga cgaaaacagc
    10261 tggcaggacg cggcgaaagc ggtgaaagcg gcaattaaac ttgccgataa agcaccggaa
    10321 agcacaccaa ctgtctatcg ccgtatcagc gaataacggt atactgatct gatcatttaa
    10381 atttgaagca ctgagtacgg agaacatatg aaacgtgcat ttattatggt gctggactca
    10441 ttcggcatcg gcgctacaga agatgcagaa cgctttggtg acgtcggggc tgacaccctg
    10501 ggtcatatcg cagaagcctg tgccaaaggc gaagctgata acggtcgtaa aggcccgctc
    10561 aatctgccaa atctgacccg tctggggctg gcgaaagcac atgaaggttc taccggtttc
    10621 attccggcag gaatggacgg caacgctgaa gttatcggcg cgtacgcatg ggcgcacgaa
    10681 atgtcatccg gtaaagatac cccgtctggt cactgggaaa tcgccggtgt cccggttctg
    10741 tttgagtggg gatacttctc cgatcacgaa aacagcttcc cgcaagagct gctggataaa
    10801 ctggttgaac gcgctaacct gcctggttat ctcggtaact gccactcttc cggtacggtc
    10861 attctggatc agctgggcga agagcacatg aaaaccggca agccgatttt ctatacctcc
    10921 gctgactccg tgttccagat tgcctgccac gaagaaacct ttggcttgga taaactctac
    10981 gaactgtgcg aaatcgcccg tgaagagctg accaacggcg gctacaacat cggtcgtgtt
    11041 atcgctcgtc cgtttattgg cgacaaagcc ggtaacttcc agcgtaccgg taaccgtcac
    11101 gacctggctg ttgagccgcc agcaccgacc gtgctgcaga aactggttga tgaaaaacac
    11161 ggtcaggtgg tttctgtcgg taaaattgca gacatctacg ccaactgcgg tatcaccaag
    11221 aaagtgaaag caactggcct ggacgcgctg tttgacgcca ccatcaaaga gatgaaagaa
    11281 gcgggtgata acaccatcgt cttcaccaac ttcgttgact tcgactcttc ctggggccac
    11341 cgtcgcgacg tcgccggtta tgctgcgggt ctggagctgt tcgaccgccg tctgccggag
    11401 ctgatgtctt tgctgcgcga tgacgacatc ctgatcctca ctgctgacca cggttgtgat
    11461 ccgacctgga ccggtactga ccacacgcgt gaacacattc cggtactggt ttacggcccg
    11521 aaagtaaaac cgggttcact gggtcaccgt gaaaccttcg cggatatcgg ccagactctg
    11581 gcaaaatatt ttggtacttc tgatatggaa tatggcaaag ccatgttctg ataatgggtg
    11641 cggcttgatg ccctcacccc ggccctctcc cacagggaga gggtgaaaac atgttgataa
    11701 ggataaaaca atggctaccc cacacattaa tgcagaaatg ggcgatttcg ctgacgtagt
    11761 tttgatgcca ggcgacccgc tgcgtgcgaa gtatattgct gaaactttcc ttgaagatgc
    11821 ccgtgaagtg aacaacgttc gcggtatgct gggcttcacc ggtacttaca aaggccgcaa
    11881 aatttccgta atgggtcacg gtatgggtat cccgtcctgc tccatctaca ccaaagaact
    11941 gatcaccgat ttcggcgtga agaaaattat ccgcgtgggt tcctgtggcg cagttctgcc
    12001 gcacgtaaaa ctgcgcgacg tcgttatcgg tatgggtgcc tgcaccgatt ccaaagttaa
    12061 ccgcatccgt tttaaagatc atgactttgc cgctatcgct gactttgaca tggtgcgtaa
    12121 cgcggtagac gcggctaaag cactgggcat tgatgctcgc gttggtaacc tgttctccgc
    12181 tgacctgttc tactctccgg acggcgaaat gttcgacgtg atggaaaaat acggcatcct
    12241 cggcgtggaa atggaagcgg ctggtatcta cggcgtcgct gcagaatttg gcgcgaaagc
    12301 cctgaccatc tgtaccgtgt ctgaccacat ccgcactcac gagcagacca ctgccgctga
    12361 gcgtcagacc accttcaacg acatgatcaa aatcgcactg gaatccgttc tgctgggcga
    12421 taaagagtaa ttgtgtttcg ctgaaaggcg attgtcgtgt gaagccggag tgggagactg
    12481 ctccggcttt ttagtatcta ttcatcaata gcaactgatt tatagattta ctattatttt
    12541 tcatatggat agaaaataaa atctattcaa gtatctattc acgaatctat tcatttatgt
    12601 tcagccgtca tccaggaagg gcatatcagc aactctggtg gacaaccata aatttctgcc
    12661 attttgcttt tgatgtttga gctaaaccgt tccgtctttt cgatctattt tagagaatta
    12721 atcgagatac ctaacatttt acttacttca gcaagcgata atttatgcct gatacgcacg
    12781 gcggctagcc aggaaatacg ctggcttaac attaaattga taatctcccg ctcctggttt
    12841 tccagatcga tcatattccc ttcctttggt attttaatca gcgagttacc ttaccgcccc
    12901 cgctatccac gtcgacaact cccgtagctc tttttcctgg tccgggaagt caaccaacag
    12961 cgcttcgcac tcttgttgca gcatatccgc gcggtacagg cagccttgca gtcgcccggc
    13021 gagggcttcc agcggcgcgg ggttgaggct gtcggtaaac acctgggcgc gggtgatatg
    13081 gcctttttca acgtcgaaat gcagttccac gccgccccag ctaaagcgtt catccagcag
    13141 atgcgagaat gccggagcct gaccgaagtt ccattcccag ctactctgac gggcaaaggt
    13201 ttcggcgaag tttggcaagt ctggcgtttt gtccggggag atgatttccg cttctacacg
    13261 ctcgccataa tgggcgaaaa aggccttggt tatggcctcg caaacctgct catgggtgat
    13321 ccccggcagc agctcggtga ggttggtcac gcgggaacgc actgaggtaa tgcctttcgc
    13381 cgccagtttc tttttatccg gattgagata gtttgccagg cggctaaggt cggcattgag
    13441 cagcaaagtg ccgtggtgga agccacgatc tttggtttcg cgataggccg agcctgagac
    13501 tttgcggtcg ccttcgactg ttttcaccac cagatcgtta cgtccggacg cttcggcgct
    13561 gacgccgagc gcgttcagcg cattgagcac aatcgacgta gagatggttt tatcgtactc
    13621 cggcttgcca gccataaagg taaagcaggt attgccgaga tcgtggaaca ccgcgccgcc
    13681 accgctactg cgccgcgcca ggcggacgtt atcttcttcc atccgccggg tattacactc
    13741 tttccacggg ttctgcgcgc gaccaatcac taccgtgtcg gcattgcgcc agagaaacag
    13801 aacgcgctgc gtggcgggca tttggcgaaa aatacactct tccaccgcca ggttaaacca
    13861 cgggtcgtaa gagtcagaga tgagcaggcg taatgtggac ataacgattt cctttcttgt
    13921 agtaatgggt cactcttttt tctcgctttc ttcctcttcc ggcaccggtt tgctggcggt
    13981 taacaggaag ggcgattgct gccagcgggt acgtttaccc tgtagcagcg tgcgggtcag
    14041 cactacgccg attgccagtg aaagcagcaa catcaggcgt aaaatgttag tggtgttatc
    14101 cacctgttgg gcttcggtgg cgagcgtatg ggtgtcgagc gtcaggcgca gatagccgag
    14161 cggtccgttt ttcccggcaa ttggttcgac aatctgctgg ttaaaatagc cgcccgcttt
    14221 tttaccgtcg agcgccagcc ggtcgcgcac ttcgacgctt tcgccagaac gtgcgataag
    14281 atcgccttgt tcgtcataca cacccgcgtc gaggatacgg ctttcatccg ttaactgatc
    14341 gaggatcgcc tgaatgcgtt tttcatccgg tgagtcggta cgcatcagcg gtgcaacgtt
    14401 cagcgttacc tgacgcgcca gggtgcgggc cagttcttcc agctgtggat tacgttgtcg
    14461 ctggtggttt tgactaaacc atgacgctcc ctgcatcagc gccactaaca aggcaagaca
    14521 gaacaggaca atcactgccc gatgcagctg gaatttcagt tttgtgcgag ccatcttcca
    14581 ccctttgaaa atttgagact taatgttgcc agaagcaatg gatacaaggt agcctcatgc
    14641 gttattttcc ctgcttcgaa cgattttaca ggagccttaa tgcctaacat tacctggtgc
    14701 gacctgcctg aagatgtctc tttatggcct ggtctgcctc tttcattaag tggtgatgaa
    14761 gtgatgccac tggattacca cgcaggtcgt agcggctggc tgctgtatgg tcgtgggctg
    14821 gataaacagc gtctgaccca ataccagagc aaactgggtg cggcgatggt gattgttgcc
    14881 gcctggtgcg tggaagatta tcaggtgatt cgtctggcag gttcactcac cgcacgggct
    14941 acgcgcctgg cccatgaagc gcaactggat gtcgccccgc tggggaaaat cccgcacctg
    15001 cgcacgccgg gtttgctggt gatggacatg gattccaccg ccatccagat tgaatgtatt
    15061 gatgaaattg ccaaactggc cggaacgggc gagatggtgg cggaagtaac cgaacgggcg
    15121 atgcgcggcg aactcgattt taccgccagc ctgcgcagcc gtgtggcgac gctgaaaggc
    15181 gctgacgcca atattctgca acaggtgcgt gaaaatctgc cgctgatgcc aggcttaacg
    15241 caactggtgc tcaagctgga aacgctgggc tggaaagtgg cgattgcctc cggcggcttt
    15301 actttctttg ctgaatatct gcgcgacaag ctgcgcctga ccgccgtggt agccaatgaa
    15361 ctggagatca tggacggtaa atttaccggc aatgtgatcg gcgacatcgt agacgcgcag
    15421 tacaaagcga aaactttgac tcgcctcgcg caggagtatg aaatcccgct ggcgcagacc
    15481 gtggcgattg gcgatggagc taatgacctg ccgatgatca aagcagcagg gctggggatt
    15541 gcctaccatg ccaagccaaa agtgaatgaa aaggcggaag tcaccatccg tcacgctgac
    15601 ctgatggggg tattctgcat cctctcaggc agcctgaatc agaagtaatt gctcgcccgc
    15661 catcctgcgg gcggcacagc attaacgagg tacaccgtgg caaaagctcc aaaacgcgcc
    15721 tttgtttgta atgaatgcgg ggccgattat ccgcgctggc aggggcagtg cagtgcctgt
    15781 catgcctgga acaccatcac cgaggtgcgt cttgctgcgt cgccaacggt ggcgcgtaac
    15841 gagcgtctca gcggctatgc cggtagcgcc ggggtggcaa aagtccagaa actctccgat
    15901 atcagccttg aagagctgcc gcgtttttcc accggattta aagagttcga ccgcgtacta
    15961 ggcggcggcg tggtgccagg aagtgccatt ctgattggcg gtaaccctgg tgcggggaaa
    16021 tccacgctgt tgctgcaaac gctgtgcaaa ctggcccagc agatgaaaac gctgtatgtc
    16081 accggcgaag agtcgctgca acaggtggca atgcgcgctc atcgccttgg cctgccgact
    16141 gacaatctca atatgttgtc ggaaaccagc atcgagcaga tctgcctgat tgccgaagaa
    16201 gagcaaccga aactgatggt aattgactcg atccaggtga tgcatatggc ggatgtacaa
    16261 tcatcgcctg gtagcgtggc gcaggtgcgt gaaacggcgg cttatctgac gcgcttcgcc
    16321 aaaacgcgcg gtgtggcgat tgtcatggtt gggcacgtaa ccaaagatgg ctcgctggct
    16381 ggccctaaag tgctggaaca ctgtatcgac tgttcggtgc ttctggatgg tgatgccgac
    16441 tcccgttttc gcaccttgcg cagccataaa aaccgcttcg gcgcggtgaa tgagctgggc
    16501 gtcttcgcga tgaccgaaca ggggctgcgt gaagtcagca acccttcggc aattttctta
    16561 agtcgcggag atgaagtgac ctccggcagc tccgtgatgg tggtgtggga aggaacgcgt
    16621 ccgttgctgg tggagattca ggcgctggtc gatcactcga tgatggcgaa tccgcgccgc
    16681 gtggcagtgg ggctggaaca aaaccgtctg gcaatcctgc tggctgtctt gcaccgtcac
    16741 ggtggtctgc aaatggccga tcaggatgta tttgtgaacg tggtcggcgg cgtgaaggta
    16801 accgaaacca gtgccgattt agcgttactg ctggcgatgg tttccagcct gcgtgacaga
    16861 ccgctgccac aggatctggt ggtgtttggt gaagtcgggc tggcagggga gatccgcccg
    16921 gtgcccagcg gtcaggaacg aatttcagaa gcggcgaaac acggttttcg ccgggcgatt
    16981 gttccggcgg ctaacgtacc gaaaaaagcg ccggaaggga tgcagatttt tggcgttaaa
    17041 aaactctccg acgcgcttag cgtgttcgac gacttataat gagagatacg gagggagata
    17101 tgtcgtcatt tgattacctg aaaactgcca tcaagcaaca gggctgcacg ctacagcagg
    17161 tagctgatgc cagcggtatg accaaagggt atttaagcca gttactgaat gccaaaatta
    17221 aaagccccag cgcgcaaaag ctggaggcgt tgcaccgttt tttggggctt gaatttcccc
    17281 gacagaagaa aaccattggt gtcgtattcg gtaagttcta cccgctgcat accggacata
    17341 tctaccttat ccagcgcgcc tgtagccagg ttgacgagct gcatatcatt atgggttttg
    17401 acgatacccg cgaccgcgcg ttgtttgaag acagcgccat gtcgcagcag cccacggtgc
    17461 cggatcgtct gcgctggtta ttacaaactt ttaaatatca gaaaaatatt cgcattcatg
    17521 ctttcaacga agagggcatg gagccgtatc cgcacggctg ggatgtgtgg agcaacggca
    17581 tcaaaaagtt tatggctgaa aaggggattc agccggacct gatctacacc tcggaagaag
    17641 ccgatgcgcc acagtatatg gaacatctgg ggatcgatac ggtgctggtc gatccgaaac
    17701 gtacctttat gagtatcagc ggtgcgcaga tccgcgaaaa cccgttccgc tactgggaat
    17761 atattcctac ggaagtgaag ccgttctttg tacgtaccgt ggcgatcctt ggtggcgagt
    17821 cgagcggtaa atccaccctg gtaaacaaac ttgctaatat cttcaatacc accagtgcgt
    17881 gggaatatgg tcgcgattat gtcttttcac acctcggcgg tgatgagatc gcattgcagt
    17941 attccgatta cgataaaatc gcgctgggcc acgcacaata cattgatttt gcagtgaaat
    18001 atgccaataa agtggcgttt attgacactg attttgtcac tacccaggcg ttctgcaaaa
    18061 agtacgaagg gcgtgagcat ccgttcgtac aggcgttgat tgatgaatac cgtttcgatc
    18121 tggtgatcct gctggagaac aacacgccgt gggtggcgga tggtttacgc agcctcggca
    18181 gttcggtgga tcgcaaagag ttccagaact tgctggtgga gatgctggaa gagaacaata
    18241 tcgaattcgt gcgggttgaa gaggacgatt atgacagccg tttcctgcgc tgcgtggagc
    18301 tggtgcggga gatgatgggg gagcagagat aaccgcgatg aaacggctca aaggcgaggt
    18361 ataaaataag ttttttgagg agttgaaaac ctctctggaa gaggctgtcg agattaaaca
    18421 aggtttaaaa gaatcagcac gggtgacccg ctacgaattt gcggatacta aggctgttgt
    18481 aggccggaaa aggcgttcac gccgtatccg gcattcggtg ctcaatgcct gatgcgacgc
    18541 ttaccgcgtc ttatcaggcc tggagattca caacgtattg aaatttcttg gtttgtaggc
    18601 cggataaggc gttcacgccg catccggcat tttacgcatt acttcgcaat acgcttgtac
    18661 ttgatacgct tcggctccag tgcgtctgcg cccagcgtgc gtttcttgta ctcttcgtac
    18721 tcagtaaagt taccttcgaa gaactcaact ttaccttcat cctggtagtc caggatgtgc
    18781 gtggcgatac ggtcgaggaa ccaacggtcg tgcgagataa ccatcgcaca gcccgggaac
    18841 tccagcaggg cgttttccag cgcgcgcagg gtttcgatat ccaggtcgtt ggttggttcg
    18901 tcgagcagca gcatgttgcc gccaacctgc agcagcttcg ccagatgcag acgaccgcgc
    18961 tcaccaccgg aaagttcacc aacgcgttta ccctgatcaa cccctttaaa gttaaagcgg
    19021 ccaacgtagg cgcggcttgg catctcggtg ttgccgatct tcataatatc cagcccgccg
    19081 gaaacttctt cccaaacggt tttgctgtta tccattgagt cacggaactg atcaaccgat
    19141 gccagtttca ccgtttcacc taaagtgatg gtgccgctgt ccggctgttc ctgaccagag
    19201 atcatacgga acagggtcga tttacccgcg ccgttcggac cgatgatccc gacgattgcc
    19261 cctttcggga tcgagaagct caggtcatca atcagcaggc gatcaccgta ggatttacgc
    19321 aggttgctga cttccagcac tttatcgccc agacgcggtc caggtggaat aaacagttcg
    19381 ttggtttcgt tacgtttctg atattcggtg ctgttcagct cttcaaagcg tgccagacgt
    19441 gctttacctt tcgactggcg gcctttagtt ccctgacgta cccactccag ctctttctcg
    19501 atcgacttac gacgcgccgc ttcttgtgaa gcttcctgcg ccaggcgctg atctttctgc
    19561 tccagccagg aggagtagtt gccttcccac ggaatacctt caccgcggtc aagttcgagg
    19621 atccagcctg caacgttatc gaggaagtaa cggtcgtgag taatcgccac aacggtgcct
    19681 tcgaagtcgt gcaggaagcg ttccagccag gccacggatt cggcatccag gtggttggtc
    19741 ggttcgtcga gcagcagcat gtctggtttt tccagaagca ggcggcacaa cgccacgcga
    19801 cgacgctcac caccggagag gttagcgatt ttcgcgtccc aatctggcag acgcagcgca
    19861 tccgccgcac gctccagctg tacgttcagg ttatgaccgt cgtgagcctg aatgatctct
    19921 tccagacggc cttgttcagc ggccagcttg tcaaaatcgg catccggatc ggcgtacagc
    19981 gcatacactt catccaggcg tttcagggcg ttaaccactt cagaaaccgc ttcttcaatg
    20041 gactcacgca cggtgtgttc cgggttcagc tgcggttcct gcggcaggta accaatcttg
    20101 atgtctggct gcggacgcgc ttcaccttcg atgtctttat caatgcccgc cataatgcgc
    20161 agcagggtag acttacccgc gccgttcaga cccaggacac caatttttgc cccagggaag
    20221 aaactcagag agatgttttt caaaatatga cgtttcggcg gaacaacttt gccgacacga
    20281 tgcatggtat aaacgaattg agccacgttg gacttcgcct ctatgtttat cgtgatgatg
    20341 agttttcaaa ggcgaagtgt agcctttttc cccgcctaat cccagttggt cgatcacctt
    20401 cgcagtaaaa gtaaaaaagt gtccgtaacg tggcgtaaac ggcaatgact ggttagcata
    20461 aatctattac gcggcatgac gctgcattga tgtatttaca cttagaggat gcgcttgtgg
    20521 aaaaagccaa acaagttacc tggcggctgt tggctgccgg tgtctgtctg ctgacggtca
    20581 gcagcgtggc gcgagccgac tcactggatg agcagcgtag ccgttacgcg caaatcaagc
    20641 aggcctggga taatcgacaa atggatgtgg tcgaacaaat gatgcctgga ctgaaggatt
    20701 atccgcttta tccctacctg gaataccgcc agatcaccga tgatctgatg aatcaaccgg
    20761 cggtgacggt cactaacttt gttcgcgcta accccacgct tcctcccgct cgcacgctgc
    20821 aatctcgttt cgtcaatgaa ctggcgcggc gtgaagactg gcgtggcttg ttagccttta
    20881 gcccggaaaa gcccggaact accgaagcgc aatgtaatta ctactatgcg aaatggaaca
    20941 ccgggcagag tgaagaagcc tggcaagggg cgaaagagct gtggctaacc ggcaagagcc
    21001 agcctaacgc ctgtgacaag ttatttagcg tctggcgtgc gtcaggtaaa caagatccgc
    21061 tggcgtattt agagcgtatc cgtctggcga tgaaagcggg taacactggc ctggtaacag
    21121 tgctggcagg gcagatgcct gccgattacc agactatcgc ctcggcaatc atttcactgg
    21181 cgaacaaccc taatacggta ctgaccttcg cgcgtacaac cggagcgacc gattttaccc
    21241 gtcaaatggc agcggtggcg tttgccagtg tggcgcggca ggatgctgaa aatgcacggc
    21301 tgatgatccc gtcgcttgcc caggcgcagc agcttaatga agatcagatt caggagctgc
    21361 gcgatatcgt cgcctggcgt ttgatgggca acgatgtcac cgacgagcag gcgaaatggc
    21421 gcgatgacgc cattatgcgc tcgcaatcca cgtcgttgat tgaacgccgt gtgcgaatgg
    21481 cacttggcac cggcgatcgt cgcggcctga atacctggct ggcgcgtctg ccgatggaag
    21541 caaaagagaa agatgaatgg cgttactggc aggcggattt attgctggaa cgcggacgtg
    21601 aagctgaagc gaaagagatt ttgcatcaac tcatgcaaca gcgtggtttc tacccgatgg
    21661 ttgcagcaca acgcatcgga gaagagtatg agatgaagat tgataaagcg ccgcagaatg
    21721 ttgacagcgc cctgactcag gggccggaga tggcgcgcgt gcgcgagttg atgtactgga
    21781 atctcgataa caccgcgcgt agcgagtggg ccaatctggt gaagagcaag tcaaaaacag
    21841 agcaggctca actggcgcgg tatgctttca acaaccaatg gtgggatctt agcgttcagg
    21901 caacgatcgc cgggaagcta tgggatcatc tggaagagcg attcccgctg gcttacaacg
    21961 atcttttcaa acgctacacc agcggtaagg agatcccgca aagctatgcg atggcgattg
    22021 cccgtcagga gagcgcctgg aatccaaaag tgaaatcacc ggtaggggcc agcggcctga
    22081 tgcagattat gcctggtaca gcgacccata cggtgaagat gttctctatt cccggttata
    22141 gtagccctgg gcaattgctg gatccggaaa cgaatatcaa cattggcacc agttatctgc
    22201 aatatgttta tcagcagttt ggcaataacc gtattttctc ctcagcagct tataacgccg
    22261 gaccagggcg ggtgcgaacc tggcttggca acagcgccgg gcgtatcgac gcagtggcat
    22321 ttgtcgagag tattccgttc tccgagacgc gtggttatgt gaagaacgtg ctggcttatg
    22381 acgcttacta ccgctatttc atgggggata aaccgacgtt gatgagcgcc acggaatggg
    22441 gacgtcgtta ctgatccgca cgtttatgat atgctatcgt actctttagc gagtacaacc
    22501 gggggaggca ttttgcttcc cccgctaaca atggcgacat attatggccc aacaatcacc
    22561 ctattcagca gcgatggcag aacagcgtca ccaggagtgg ttacgttttg tcgacctgct
    22621 taagaatgcc taccaaaacg atctccattt accgttgtta aacctgatgc tgacgccaga
    22681 tgagcgcgaa gcgttgggga ctcgcgtgcg tattgtcgaa gagctgctgc gaggcgaaat
    22741 gagccagcgt gagttaaaaa atgaactcgg cgcgggcatc gcgacgatta cgcgtggatc
    22801 taacagcctg aaagccgcgc ctgtcgagct gcgccagtgg ctggaagagg tgttgctgaa
    22861 aagcgattga ttttgttgcc tggtaagacg cgtcagcgtc gcatcaggcg cttaatacac
    22921 cgcattatga aacggactca gcgccaggat caccgcctgg tgatagacgc tggcgcgagt
    22981 gagtttcccg gcggtaaaca cgccgatcgc cccttcctta cgaccaatct catcaatacc
    23041 ggtataacgc gacattacgg gaccaagtgc ctcaccttca cgcacttttt ccagaatcac
    23101 cgcaggcaac ggcaaagtag ccgaacgcgc ctcgccgcgc tggctggcgt tttcaatcac
    23161 cacccaactg aaagtgctgt caccatcgat gccagcttca atcgccaccc aaaaatcagc
    23221 ctctggaagt aaacggcggg catttgctac ccgatttcgt gcgccagcgc gcgtttcctc
    23281 actgccaaag ggttgttccg gtacaccgct ctcgacggca acggatgcaa tatggcagga
    23341 tccttcgccg aagatctcgt gaaatgcctg cagaatggcc tgaattttag cgggattggt
    23401 ggtcgcacag acaacttggt gcataatcag cattactcag aaaattaacg ttacagcagt
    23461 atacggaaaa aaagcatgtt acaggtatac ctagtccgcc acggtgaaac gcagtggaac
    23521 gccgagcgac gtattcaggg ccagtctgac agcccgctga ccgccaaagg tgagcaacag
    23581 gcgatgcagg tggcaacccg tgccaaagag cttggcatta cgcatatcat cagtagcgat
    23641 ttaggacgca cccggcgtac ggcggaaatc atcgcccagg cctgcggctg tgacatcatc
    23701 tttgattctc gcctgcgtga attaaacatg ggtgtgctgg aaaaaagaca tatcgattct
    23761 ctgaccgaag aagaagagaa ctggcgtcgg cagctggtca atggcaccgt tgacgggcgt
    23821 attcctgaag gcgagtcaat gcaggagctc agcgatcgcg tcaatgcggc actggaatct
    23881 tgccgggact taccgcaggg aagccgaccg ctgctggtga gccatggtat tgcactggga
    23941 tgtctggtga gtacgattct cggattacca gcatgggcag agcgccgctt acgtctgcgt
    24001 aactgttcta tttcgcgcgt ggattatcag gaaagcctgt ggctggcgtc tggctgggtt
    24061 gttgaaaccg caggggacat ctcgcatctg gacgcccctg cattagatga gttgcagcgt
    24121 taacgacgga tcggaatcag cagttcacag cgcagattaa ttgggcgatc tcccgctttg
    24181 gcatcttctg ccgggtagta tcgctcaata tcctgacctt tacggcgcgt cagattgagc
    24241 attggcatgc acgttccgta taccgtcagg ataaactcct gcacgccggt tcccagacct
    24301 tcataggtaa acatcacata ttcgccgccc tgcagcatca ccggatgccc cgtcagtaca
    24361 tagccatctg cctgatcctg ggctaacgcg gtggtataga atacctcttg ttcgtcgtct
    24421 ttatcctgac tcggacgcgt ttcattcagg ccgtagagca ccggcggaat ggtcggcgcg
    24481 ttgccgagaa aatcgtgcca gaactgataa cgcatttcat ggcggaaatc agagatttgc
    24541 tccagcgaac aggagtagct ctgggtaaca ccaatcagcg gcgtatcttc cagggtgaca
    24601 aatttgtgct ctggcattgt gaattcaccc agacgtagcg gcgggcgaat accaaaggcg
    24661 ctccattcag gagaacggcg gtaaagtgca ggagtctggg caaactgctt cttgaatgcg
    24721 cgggtaaatg tctgttgaga gtcgaagcgg tattgcagcg cgatgtccag aatcggacgc
    24781 gcagtcaggc gtagtgcgac cgccgatttc gacaaacgac gagcacgaat atacgcgcca
    24841 atagcatggc cagtgacatc tttaaacatt ctctgtaagt gccacttgga ataacctgct
    24901 ttcgccgcta cattgtcgag cgacaggggc tgatccagat gaccttccag ccagattaaa
    24961 aggtcgcgaa taatgccggc ctgatccata aaatatcctc atcctttcaa caacgagcac
    25021 ctgacatcag gtaattggat aatagcattt tttgctgttt tagcattcag tgtt
//
LOCUS       DAZKIV010000061        24801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZKIV010000061 DAZKIV010000000
VERSION     DAZKIV010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.061"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..153)
                     /locus_tag="ACHOT6_002389"
     CDS             complement(4..153)
                     /locus_tag="ACHOT6_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947593.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            462..1601
                     /gene="wza"
                     /locus_tag="ACHOT6_002390"
     CDS             462..1601
                     /gene="wza"
                     /locus_tag="ACHOT6_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFP9947594.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1607..2050
                     /gene="wzb"
                     /locus_tag="ACHOT6_002391"
     CDS             1607..2050
                     /gene="wzb"
                     /locus_tag="ACHOT6_002391"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFP9947595.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2053..4215
                     /gene="wzc"
                     /locus_tag="ACHOT6_002392"
     CDS             2053..4215
                     /gene="wzc"
                     /locus_tag="ACHOT6_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFP9947596.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLTKEGVTLMVEAIHASRGSEFTITKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4393..5232
                     /gene="wcaA"
                     /locus_tag="ACHOT6_002393"
     CDS             4393..5232
                     /gene="wcaA"
                     /locus_tag="ACHOT6_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFP9947597.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5235..5723
                     /gene="wcaB"
                     /locus_tag="ACHOT6_002394"
     CDS             5235..5723
                     /gene="wcaB"
                     /locus_tag="ACHOT6_002394"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFP9947598.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     AENMACPHIGNGVELGANVIILGDITIGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5720..6937
                     /gene="wcaC"
                     /locus_tag="ACHOT6_002395"
     CDS             5720..6937
                     /gene="wcaC"
                     /locus_tag="ACHOT6_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFP9947599.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLINYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6912..8129
                     /gene="wcaD"
                     /locus_tag="ACHOT6_002396"
     CDS             6912..8129
                     /gene="wcaD"
                     /locus_tag="ACHOT6_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFP9947600.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGMEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8140..8886
                     /gene="wcaE"
                     /locus_tag="ACHOT6_002397"
     CDS             8140..8886
                     /gene="wcaE"
                     /locus_tag="ACHOT6_002397"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFP9947601.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIFNLRFISEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKS"
     gene            8902..9450
                     /gene="wcaF"
                     /locus_tag="ACHOT6_002398"
     CDS             8902..9450
                     /gene="wcaF"
                     /locus_tag="ACHOT6_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFP9947602.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETK"
     gene            9477..10598
                     /gene="gmd"
                     /locus_tag="ACHOT6_002399"
     CDS             9477..10598
                     /gene="gmd"
                     /locus_tag="ACHOT6_002399"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFP9947603.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10601..11566
                     /gene="fcl"
                     /locus_tag="ACHOT6_002400"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10601..11566
                     /gene="fcl"
                     /locus_tag="ACHOT6_002400"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFP9947604.1"
                     /translation="MSRQRIFIAGHRGMVGSAITRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNESYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11569..12048
                     /gene="gmm"
                     /locus_tag="ACHOT6_002401"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11569..12048
                     /gene="gmm"
                     /locus_tag="ACHOT6_002401"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFP9947605.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            12045..13268
                     /gene="wcaI"
                     /locus_tag="ACHOT6_002402"
     CDS             12045..13268
                     /gene="wcaI"
                     /locus_tag="ACHOT6_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFP9947606.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNIIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRLALLLPKYNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13271..14707
                     /gene="cpsB"
                     /locus_tag="ACHOT6_002403"
     CDS             13271..14707
                     /gene="cpsB"
                     /locus_tag="ACHOT6_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFP9947607.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14900..16270
                     /gene="cpsG"
                     /locus_tag="ACHOT6_002404"
                     /gene_synonym="manB"
     CDS             14900..16270
                     /gene="cpsG"
                     /locus_tag="ACHOT6_002404"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFP9947608.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVERTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16325..17719
                     /gene="wcaJ"
                     /locus_tag="ACHOT6_002405"
     CDS             16325..17719
                     /gene="wcaJ"
                     /locus_tag="ACHOT6_002405"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFP9947609.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17721..19199
                     /gene="wzxC"
                     /locus_tag="ACHOT6_002406"
     CDS             17721..19199
                     /gene="wzxC"
                     /locus_tag="ACHOT6_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFP9947610.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGIVVCVAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGASVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIATGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19475..20755
                     /gene="wcaK"
                     /locus_tag="ACHOT6_002407"
     CDS             19475..20755
                     /gene="wcaK"
                     /locus_tag="ACHOT6_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFP9947611.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKHSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20752..21972
                     /gene="wcaL"
                     /locus_tag="ACHOT6_002408"
     CDS             20752..21972
                     /gene="wcaL"
                     /locus_tag="ACHOT6_002408"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFP9947612.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKP
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21983..23377
                     /gene="wcaM"
                     /locus_tag="ACHOT6_002409"
     CDS             21983..23377
                     /gene="wcaM"
                     /locus_tag="ACHOT6_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFP9947613.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPSGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23552..24445
                     /gene="galF"
                     /locus_tag="ACHOT6_002410"
     CDS             23552..24445
                     /gene="galF"
                     /locus_tag="ACHOT6_002410"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFP9947614.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6092 a         6198 c         6730 g         5781 t
ORIGIN      
        1 ttgtcaacct aaagaaactc ctaaaaacca tattgaatga cacttaatat aattcttaaa
       61 aatagccaat tgccgaattg ttaccttgcc tgctattccg ttagctgtaa cacttcctcc
      121 tgcattattg gagagccaat attcaattac cataatgtca ccttatattc ggaagttaat
      181 ttccgcaata accctttctc atcaacgatt gtatgcagag agggtttgaa aaattaagct
      241 aaatcagtgt attggtagct aaaaagccag gggcggtagc gtgtctggat gcctgaaaga
      301 ccagtctgaa ttattctgtc aatagcctgc ggatgaaatc aattttttta ggactgatgc
      361 cagttaaatt tgtattcaat taactgcaag gtaaatgtgc atggcgacat tgctattaat
      421 agtgcacagg ataattactc tgccaaagtg ataaataaac aatgatgaaa tccaaaatga
      481 aattgatgcc attattggtg tcagtaacct tgataagcgg ttgcacagta cttccgggca
      541 gcaatatgtc gacgatgggc aaagacgtca tcaaacagca ggacgctgat ttcgatctcg
      601 acaaaatggt gaatgtttat ccgctgaccc cgcgcctgat tgaccaatta cgcccacgcc
      661 cgaatgtagc gcgccccaat atgacgctgg aaagtgagat cgcgaattac cagtatcgcg
      721 tcgggccggg cgacgttctg aatgtcaccg tctgggatca cccggaactc accacgccag
      781 ccggtcagta ccgcagctcc agcgacaccg gcaactgggt acagcctgac ggcactatgt
      841 tttacccgta tatcggcaag gtccacgtag tcgggaaaac gctcgctgaa atccgcagtg
      901 atattaccgg gcgcttagcg acgtacatcg ctgacccgca ggtggatgtt aatatcgccg
      961 ccttccgctc gcaaaaggcc tatatctcag gtcaggtgaa taaatccggt caacaggcga
     1021 tcaccaacgt accactgacc attctcgacg ccatcaacgc cgctggtggc ctgaccgaca
     1081 ccgctgactg gcgcaacgtg gtgctaacac acaatggtcg tgaagaacgc atttctttgc
     1141 aggcgctgat gcaaaacggc gacctcaatc agaaccgact gctttacccc ggcgatattc
     1201 tctacgtgcc gcgtaatgat gatctgaaag tgtttgtgat gggtgaagtg aagaaacaga
     1261 gcaccctgaa aatggacttt agcggcatga ccctgactga agccctgggc aatgccgaag
     1321 gtatcgacat gaccacctcc aacgccagcg gcatctttgt cattcgtccg ctgaaaggcg
     1381 agggcgggcg taacggcaag attgccaata tctaccagct ggatatgtcc gatgcgacgt
     1441 cgctggtgat ggcgacagaa ttccgcctgc aaccttatga cgtggtgtat gtcaccaccg
     1501 ccccggtttc ccgctggaac cgtctgatca atcagttgct gccaactatt agcggtgtcc
     1561 gttacatgac ggatacagcc agcgacattc ataactggta atcgtcatgt ttaacaacat
     1621 attagttgtc tgtgtcggca atatttgccg ttccccgacg gcggaacgct tactgcaacg
     1681 ttatcacccg gagctgaaag tggagtccgc tggactcggc gcgctggtcg gtaagggcgc
     1741 tgatcctacc gctatcagcg tcgccgcaga acatcaactg tctctggaag gtcactgtgc
     1801 acggcaaatc agccgccgcc tgtgtcgcaa ctatgacctg attttgacca tggaaaagcg
     1861 ccatatcgaa cgcttatgcg agatggcacc tgagatgcgc ggcaaagtga tgctgtttgg
     1921 tcactgggat aacgaatgtg aaatccccga tccgtatcgc aaaagccggg aaacgtttgc
     1981 agcggtgtac acattacttg aacggtctgc ccgccagtgg gcgcaggcat tgaacgcaga
     2041 gcaggtataa gaatgacaga aaaagtaaaa caacatgccg ctccggtaac gggcagtgat
     2101 gaaatcgata ttggtcgcct ggtcggcacc gtcattgaag cgcgctggtg ggtgattggc
     2161 atcaccgctg tatttgccct ctgtgctgtg gtttacactt tcttcgccac gccgatttat
     2221 agtgccgacg cactggtaca aatcgaacaa aacagcggca attcgttagt gcaggacatt
     2281 ggctcggcat tagccaacaa accgcctgca tcggacgccg agatccagtt gattcgttcg
     2341 cgtctggtgc ttggtaaaac ggtggacgat ctagacctcg atattgcggt gagcaaaaac
     2401 acgttcccga ttttcggtgc gggctgggat cgcctgatgg gacgtcagaa cgaaacggtg
     2461 aaagtgacca cctttaaccg tccgaaagag atggcggatc aggtgtttac gcttaatgtg
     2521 ctggacgata aaaactacac tctgagcagc gatggcggct ttagcgcccg tgggcaagcg
     2581 ggccagatgc tgacaaaaga aggcgtcacg ctgatggttg aagctattca cgctagccgg
     2641 ggcagtgagt ttaccatcac caaatactcc acgctgggga tgatcaacca actgcaaaac
     2701 agcctgacgg taacggagaa cggcaaagac gcaggcgtac tgagcctgac ttataccggt
     2761 gaagatcgcg aacagatccg cgacattctt aacagcatcg cccgtaacta tcaggaacaa
     2821 aatattgagc gcaaatcggc ggaagcgtcg aaaagcctcg ctttcctcgc gcaacagtta
     2881 ccggaagtac gtagccgcct cgatgttgcc gaaaacaaac tgaatgcctt ccgtcaggat
     2941 aaagattctg ttgatctgcc gcttgaagcg aaagcggtgc tcgattcgat ggtgaacatc
     3001 gacgcgcagt tgaacgaact gacctttaaa gaggcggaaa tctccaaact gtacaccaaa
     3061 gttcacccgg cgtaccgcac gctgctggag aaacgtcagg cgctggaaga agagaaagcc
     3121 aaacttaacg gtcgcgtaac ggcgatgccg aaaacccagc aggaaattgt ccgtctgacc
     3181 cgcgatgtcg agtctggtca gcaggtctat atgcaactgc tgaataaaga gcaggagctg
     3241 aaaatcaccg aagccagcac cgtcggcgat gtgcgcattg ttgacccggc aatcactcag
     3301 cctggtgtgc taaaaccgaa gaaagggctg attatcctcg gcgcgattat ccttggcctg
     3361 atgctctcta tcgtgggtgt gctgctgcgc tcgttgttta atcgcggcat cgaaagcccg
     3421 caagtgctgg aagaacacgg aatcagcgtc tatgccagca tcccgctgtc ggagtggcag
     3481 aaagcgcgcg atagcgtcaa aaccatcaaa ggggttaaac gctataaaca gagccagcta
     3541 ctggcagtgg ggaatccaac cgatctggcg attgaagcca tccgtagtct gcgtaccagt
     3601 ctgcacttcg cgatgatgca ggcgcagaac aatgtgttga tgatgaccgg ggttagcccg
     3661 tcaattggta aaacctttgt ctgcgccaac ctggcggcgg tgatcagcca gaccaataaa
     3721 cgggtgttgt tgatcgactg tgatatgcgc aaaggctaca cccacgagct gttgggcacc
     3781 aataacgtca atggtctgtc ggaaattctg attggtcagg gcgatattac taccgctgct
     3841 aaaccgacct ctattgccaa atttgacctg atcccgcgcg gtcaggtgcc gccaaatcct
     3901 tctgagctgt tgatgagcga acgctttgcc gaactggtga actgggcgag taaaaactat
     3961 gacctggtgt tgattgatac gccgccgatt ctggcagtga ccgatgccgc aattgttggt
     4021 cgtcatgtcg gaaccacgtt aatggtggcg cgttatgcgg tcaacacatt gaaagaagtg
     4081 gaaaccagtc tgagccgctt tgagcaaaac ggtattccgg tgaaaggggt gattctgaac
     4141 tccatcttcc gccgcgccag cgcgtatcag gattatggct attacgaata cgaatataag
     4201 tcggatgcga aataacgaca tccgctgtcg gatgcgatgc tgacgcatct tatccgacct
     4261 accttcacgc cgcatccggc aagcaaacca gctccgtagg ccggataagg cgctcacgcc
     4321 gcatccggca acagaacccg taaacaagct tataaaccgg cagaacatct cgacactccg
     4381 ggagaacaac ctatgaaaga caatccgctg atttcaatct atatgccgac ctggaaccgc
     4441 caacaactgg cgattcgggc gataaaatcg gtcctgcgcc aggactacag caactgggag
     4501 atgatcatcg tggatgattg ctccacctcc tgggaacaac tgcaacagta cgtcactgcc
     4561 ctcaacgatc cgcgtattac ctacattcac aacgacatta acagcggggc gtgcgcggta
     4621 cgtaaccagg cgattatgct ggcgcaaggg gaatacatta ccgggatcga tgacgatgat
     4681 gaatggacgc ccaaccgcct gagcgtcttc ctcgcccata aacagcaact ggtcactcac
     4741 gcctttttgt acgctaacga ctacgtatgc cagggagaag tttattccca accggcaagc
     4801 ttaccactgt atcccaaatc gccgtactca cgccgcctgt tctataaacg caatatcatt
     4861 ggcaatcagg tttttacctg ggcgtggcgt ttcaaagagt gcttgttcga taccgaactg
     4921 aaagcagcac aggattacga catcttcctg cggatggtgg tggagtatgg cgaaccgtgg
     4981 aaagtggaag aggcgacgca gatcctgcat atcaatcacg gtgaaatgca gatcacctcg
     5041 tcaccgaaaa aattctccgg gtacttccac ttttaccgca agcacaaaga taaatttgac
     5101 cgtgcaagca aaaaatatca gctgtttacc ctctaccaga tccgcaataa acgtatgacc
     5161 tggcgcactt tgctgacgct gctgtcggtg cgtaacggca aacgtctggc tgatggtatt
     5221 cgggggcgct aaagatgctg gaagatctgc gtgccaacag ctggagttta cgcccgtgct
     5281 gcatggttct tgcctatcgt gttgctcatt tttgttcggt gtggcgcaaa aagaacgtcc
     5341 tcaacaatct gtgggcggcc ccgctgctgg tgctgtatcg cattatcacc gaatgctttt
     5401 tcggttatga aatccaggcc gccgcgacca ttggccgccg ctttactatc catcacggtt
     5461 acgccgtagt gatcaataaa aacgtagtag cgggggatga ttttaccatt cgccacggcg
     5521 tcactatcgg caatcgtggt gccgaaaaca tggcatgtcc acacattggc aacggcgtcg
     5581 aactcggtgc caacgttatt attcttggtg atatcacgat tggtaacaac gtcaccgtgg
     5641 gcgcgggcag cgtggtgctt gactctgtcc cggacaacgc gctggtggtg ggagaaaaag
     5701 cgcgagtgaa ggtaatcaaa tgaatattct gcaatttaat gtgcgactgg cggaaggcgg
     5761 ggcagcaggt gtggcgttag atctccacca gcgtgcgctg caacaggggc tggcgtcaca
     5821 ttttgtctac ggctacggca aaggcggcaa agagagcgtc agccatcaga actatccgca
     5881 ggtcatcaaa catacgccgc ggatgaccgc gatggcgaac attgccctgt ttcgtctgtt
     5941 taatcgcgat ctgtttggca atttcaatga gttatatcgc accattactc gtacaccggg
     6001 tccggtggtc ctgcattttc atgtgctgca cagctactgg ctaaatctta agagcgtggt
     6061 gcgcttttgc gaaaaggtga aaaaccacaa accggatgtc actctggtct ggacgctgca
     6121 cgaccactgg agcgttaccg ggcgctgcgc ctttaccgac ggttgtgaag gctggaaaac
     6181 gggctgccag aaatgcccga ccttaattaa ttatccgccg gtgaagattg atcgcgcaca
     6241 ccagctggtg gcgggcaaac gccagttatt ccgtgagatg ctggcgctgg gctgtcagtt
     6301 tatttccccc agccagcatg tggctgacgc tttcaatagc ctgtacggtc cagggcgttg
     6361 ccggattatc aataatggca ttgatatggc aaccgaagcg attctggcgg acttgcctcc
     6421 ggtgcgcgaa acccagggta agccgaaaat cgcggtggtg gcgcatgacc tgcgttacga
     6481 cggcaaaact aaccagcaac tggtgcgcga gatgatggcg ctgggcgaca aaatcgaact
     6541 gcataccttt ggtaagttct cgccgttcac cgctggcaac gtggttaatc acggctttga
     6601 aactgacaag cgcaagttga tgagcgcgct caatcagatg gatgcgctgg tgttcagttc
     6661 tcgcgtcgat aactacccgc tgattttgtg tgaggcgcta tcgattggcg tgccggtgat
     6721 tgccacccat agcgatgcgg cgcgggaagt gttgcaaaaa tccggcggta aaaccgtcag
     6781 cgaagaagag gtgctgcaac tggtgcagtt aagcaaaccg gaaatcgcgc aggcgatatt
     6841 tggtaccacg ctggctgagt tcagccaacg cagccgcgcc gcctacagtg gacaacagat
     6901 gctggaggag tatgtcaact tctatcagaa tctgtagcta cctgctgctg ccgctgatct
     6961 acctgctggt taacgtcaaa atcgcccagc ttggcgaaag tttccccatc actatcgtca
     7021 cttttttacc tgtcttgtta ctgctgtttt tagaacgcat cagcgttaaa aaattgatga
     7081 ttgccttagg gattggtgcg ggactcacgg cgtttaacta cctgtttggt cagtcgctgg
     7141 atgccagtaa atacgtcact tcaaccatgc tgtttgtcta tattgtgatc attattggca
     7201 tggtgtggag tattcgtttt aaaacaattt cgccacacaa ccatcgcaag atattacgtt
     7261 tcttttatct ggtggtcggg ctggtggtgg cgctggcggc ggtggagatg gcgcaaatta
     7321 tcctcaccgg cggcagcagt attatggagt cgatttcgaa atatctgatt tacagcaaca
     7381 gctatgtact gaatttcatt aaattcggcg gtaagcgtac gacagcactt tatttcgaac
     7441 cggcattttt cgctctggca ttaatctcaa tttggctcag catcaaacag tttggtatca
     7501 aaacgcctaa aaccgatgct atgattctcg caggaataat attatccgga tcgttttcag
     7561 gggttatgac ctttatcctg ttttatttgc tggagtgggc attccaatat ctgaataaag
     7621 aggcgattaa gaaaaagttg ccgttagcat tgatttctct ggctgtattc ctggttggtg
     7681 tggtaatcgc gtttccttat atttccaccc gtctgggcga tttaggtatg gaaggatcgt
     7741 catcgtatta tcgtattgtc ggtccgctgg taatggtcgg ttattctttg acccatattg
     7801 acggtgtagt cagatttggc tcactttatg aatatgtcgc atcattcgga atatttaacg
     7861 gtgcggatgt cggaaaaacc atagacaatg gtttgtatct gctgattatt tatttttcct
     7921 ggttcgcagt gtttttatcg ctgtggtaca tggggaaagt gataaaaatg atgatcaacg
     7981 cttttggtga taaccgcaat tttcgcgtgc aattatatct tttcactccg gtatcgctgt
     8041 tttttaccgg ttcgatattt agcccggaat atgcattttt aatcgtctgt ccgtttattt
     8101 tgcgaaaagc gttaaatatt acgaggtaag aataagaaca tgttgcttag cataatcact
     8161 gtcgcgtttc gtaacctcga agggatagtc aaaacacatg cctcgctggc gcatctggcg
     8221 caggcggaag atatcagctt cgaatggatt gttgtcgatg gcggttccaa cgacggcacc
     8281 cgtgagtatc tggaaaatct caatggtatc tttaacctac gctttatcag cgagccagat
     8341 aacggtatct acgacgccat gaacaaaggc attgcgatgg cacaaggcaa gttcgcgttg
     8401 tttctcaact cgggcgatat ttttcatcag gatgccgcaa attttgtccg taagttaaaa
     8461 atgcaaaaag ataacgtgat gatcaccggc gatgcgctgc tggattttgg cgacgggcat
     8521 aaaattaagc gtagtgctaa accgggttgg tatatttatc acagcctgcc cgccagccat
     8581 caggcgatat ttttcccggt atccggcttg aaaaaatggc gttatgacct ggaatataaa
     8641 gtatcttccg attacgcgct ggcggctaaa atgtataaag ccggttatgc atttaaaaaa
     8701 ctcaatggcc tggtgtctga attttccatg ggtggggtat caaccaccaa taatatggaa
     8761 ttgtgtgctg acgccaaaaa agtccaacgg caaatattac atgtgcctgg cttctgggca
     8821 gaattatcct ggcatttacg ccaacgtact acctcaaaga cgaaagcctt atataacaaa
     8881 agctgaatat aagggaaaac gatgcaagat ttaagcggtt tctcggtgcc gaaagggttc
     8941 cggggcggca acgctattaa agtgcaatta tggtgggcag tacaggcaac aatatttgcc
     9001 tggtcgccac aagtattgta tcgctggcgg gcttttttat tacgtttatt cggcgcaaaa
     9061 ataggaaaaa acgtagttat tcgtccgtca gtaaaaatta cctatccgtg gaaattaacc
     9121 ttaggtgatt acgcgtgggt cggcgatgac gtcaatttat ataccctcgg tgaaataacc
     9181 attggcgcac attcggtgat atcgcaaaaa agttatttat gcaccggtag ccacgaccat
     9241 gcaagtcaac atttcaccat taatgccacg cctattgtga ttggcgagaa atgctggctg
     9301 gcaaccgatg tttttgttgc ccctggcgtc acgattggcg acggcaccgt cgtgggtgca
     9361 cgaagcagtg tttttaaatc gcttccggca aatgtggttt gccgggggaa tcccgcagtg
     9421 gtgatacgcg aacgcgttga aactaaataa attcaaaaaa tacagaggaa taagacatgt
     9481 caaaagtcgc tctcatcacc ggtgtaaccg gacaagacgg ttcttacctg gcagagtttc
     9541 tgctggaaaa aggttacgag gtgcatggta ttaagcgtcg tgcatcgtca ttcaacaccg
     9601 agcgcgtgga tcacatttat caggatccgc acacctgcaa cccgaaattc catctgcatt
     9661 atggcgacct gagtgatacc tccaacctga cgcgcatttt gcgtgaagtg cagccggatg
     9721 aagtgtacaa cctgggcgca atgagccacg ttgcggtgtc ttttgagtca ccggaatata
     9781 ccgctgacgt cgacgcgatg ggtacgctgc gcctgctgga ggcgatccgc ttcctcggtc
     9841 tggaaaagaa aacccgtttc tatcaggctt ccacctctga actgtacggt ctggtgcagg
     9901 aaattccgca gaaagaaacc acgccgttct acccgcgatc tccgtatgcg gtcgccaaac
     9961 tgtatgccta ctggatcacc gttaactacc gcgaatccta cggcatgtac gcctgtaacg
    10021 gtattctctt caaccatgaa tccccgcgcc gcggcgaaac cttcgttacc cgcaaaatca
    10081 cccgcgcaat cgccaacatc gcccaggggc tggagtcgtg cctgtacctc ggcaatatgg
    10141 attccctgcg tgactggggc cacgccaaag actacgtaaa aatgcagtgg atgatgctgc
    10201 aacaggaaca gccagaagat ttcgttatcg cgaccggcgt tcagtactcc gtacgtcagt
    10261 tcgtggaaat ggcggcagca cagctgggca tcaaactgcg ctttgaaggc acgggcgttg
    10321 aagagaaggg cattgtggtt tccgtcaccg ggcatgacgc gccgggcgtt aaaccgggcg
    10381 atgtgattat cgccgttgac ccgcgttact tccgtccggc ggaagttgaa acgctgctcg
    10441 gcgacccgac caaagcgcac gaaaaactgg gctggaaacc ggaaatcacc ctcagagaga
    10501 tggtgtctga aatggtggct aatgacctcg aagcggcgaa aaaacactct ctgctgaaat
    10561 ctcacggcta cgacgtggcg atcgcgctgg agtcataagc atgagtagac aacgcatttt
    10621 tatcgctggc catcgcggaa tggtcggttc cgccatcacg cggcagctcg aacagcgcgg
    10681 tgatgtggaa ctggtattac gcacccgcga cgagctgaac ctgctggaca gccgcgcggt
    10741 gcatgatttc tttgccagcg aacgcattga ccaggtctat ctggcggcgg cgaaagtggg
    10801 cggcattgtt gccaacaaca cctatccggc ggatttcatc taccagaaca tgatgattga
    10861 gagcaacatc attcacgccg cgcatcagaa cgacgtgaac aaactgctgt ttcttggatc
    10921 gtcctgtatc tacccgaaac tggcaaaaca gccgatggca gaaagcgagt tattgcaggg
    10981 cacgctggag ccgaccaacg agtcttacgc cattgccaaa atcgccggga tcaaactgtg
    11041 cgaatcatac aaccgccagt acggacgcga ttaccgctca gtcatgccga ccaacctgta
    11101 tggcccgcat gacaacttcc acccgagtaa ttcgcatgtg atccctgcat tgctgcgtcg
    11161 cttccacgag gcgacggcac agaatgcacc ggacgtggtg gtatggggca gcggtacacc
    11221 gatgcgtgaa ttcctgcacg tcgatgatat ggcggcggcg agcattcatg tcatggagct
    11281 ggcgcacgaa gtctggctgg agaacaccca gccgatgctg tcgcacatta acgtcggcac
    11341 gggcgttgac tgcactatcc gcgagctggc gcaaaccatc gccaaagtgg tgggttacaa
    11401 aggtcgggtg gtttttgatg ccagcaaacc ggatggtacg ccgcgcaaac tgcttgatgt
    11461 gacgcgcctg catcagcttg gctggtatca cgaaatctca ctggaagcgg ggcttgccag
    11521 cacttaccag tggttccttg agaatcaaga ccgctttcgg gggtaatgat gtttttacgt
    11581 caggaagact ttgccacggt agtgcgctcc accccgcttg tctctctcga ctttattgtc
    11641 gagaacagtc gcggcgagtt tctgcttggc aaaagaacca accgcccggc gcagggttac
    11701 tggtttgtgc cgggagggcg cgtgcagaaa gacgaaacgc tggaagccgc atttgagcgg
    11761 ctgacgatgg cggaactggg gctgcgtctg ccgataacag caggccagtt ttacggtgtc
    11821 tggcagcact tttatgacga taacttctcc ggcacggatt tcaccactca ctatgtggtg
    11881 ctcggttttc gcttcagagt agcggaagaa gaactgttac tgccggatga gcagcatgac
    11941 gattaccgct ggctgacgcc ggacgcactg ctcgccagtg acaatgttca cgctaacagc
    12001 cgcgcctatt ttctcgctga gaagcgtgcc ggagtacccg gattatgaaa atactggtct
    12061 acggcattaa ctactcgccg gagttaaccg gcatcggcaa atacaccgga gaaatggtgg
    12121 aatggctggc ggcgcaaggt catgaggtgc gggttattac cgcaccgcct tactacccgc
    12181 agtggcaggt gggcgagaac tattccgcct ggcgctacaa acgagaagag ggggccgcca
    12241 cggtgtggcg ctgcccgctg tacgtgccaa aacagccgag caccctgaaa cgcctgttgc
    12301 atctcggcag ttttgccgtc agcagttttt tcccgttgat ggcgcaacgt cgctggaagc
    12361 cggatcgcat tatcggcgtg gtgccaacgc tgttttgcac gccgggaatg cgcctgctgg
    12421 cgaaactctc tggtgcgcgt accgtgctgc atattcagga ttacgaagtg gacgccatgc
    12481 tggggctggg ccttgccgga aaaggcaaag gcggcaaagt ggcacagctg gcaacggcgt
    12541 tcgaacgtag cggactgcat aacgtcgata acgtctccac gatttcgcgt tcgatgatga
    12601 ataaagccat cgaaaaaggc gtggcggcgg ataacatcat tttcttcccc aactggtcgg
    12661 aaatcgcccg ttttcagcat gttgcagacg ccgatgttga tgcccttcgt aaccagcttg
    12721 gcctgccgga taacaaaaaa atcattcttt actccggcaa tattggtgaa aagcagggac
    12781 tggaaaacgt tattgaagct gccgatcgcc tgcgcgatga accgctgatt tttgccattg
    12841 tcgggcaggg cggcggcaaa gcgcggctgg aaaaaatggc gcagcagcgt ggactgcgca
    12901 acatgcaatt tttcccgctg caatcgtatg acgctttacc cgcactgctg aagatgggcg
    12961 attgccatct ggtggtgcaa aaacgcggcg cggcagatgc cgtattgccg tcgaaactga
    13021 ccaatattct ggcagtaggc ggtaacgcgg tgattaccgc agaggcccac acagaactgg
    13081 gacagctttg cgaaaccttt ccgggcattg cggtttgcgt ggaaccggaa tcggtcgagg
    13141 cgctggtggc ggggattcgt ctggcgctcc tgctgcccaa gtacaacacg gtggcacgtg
    13201 aatatgccga acgcacgctc gataaagaga acgtgttacg tcaatttata aatgatattc
    13261 ggggataatt atggcgcagt cgaaactcta tccagttgtg atggcaggtg gctccggtag
    13321 ccgcttatgg ccgctttccc gcgtacttta ccccaagcag tttttatgcc tgaaaggtga
    13381 tctcaccatg ctgcaaacca ccatctgtcg cctgaacggc gtggagtgcg aaagcccggt
    13441 ggtgatttgc aatgagcagc accgctttat tgtcgcggaa cagctgcgtc aactgaacaa
    13501 actcaccgag aacattattc tcgaaccggc agggcgtaac actgcacctg ccattgcgct
    13561 ggcggcgctg gcggcaaaac gtcatagccc ggagagcgac ccgttaatgc tggtgctggc
    13621 ggcggatcat gtgattgccg atgaagacgc gttccgtgcc gccgtgcgta atgccatgcc
    13681 atatgccgaa gcgggcaagc tggtgacctt tggcattgtg ccggatctac cagaaaccgg
    13741 ttatggctat attcgtcgcg gtgaagtgtc tgcgggtgag caggatacgg tggcctttga
    13801 agtggcgcag tttgtcgaaa aaccgaatct ggaaaccgcc caggcctatg tggcaagcgg
    13861 cgaatattac tggaacagcg gcatgttcct gttccgcgcc ggacgctatc tcgaagaact
    13921 gaaaaaatat cgcccggata ttctcgatgc ctgtgaaaaa gcgatgagct ccgtcgatcc
    13981 ggatcttgat tttattcgtg tggatgaaga agcgtttctc gcctgcccag aagagtcggt
    14041 ggattacgcg gtcatggaac gtacggcaga tgccgttgtg gtaccgatgg atgcgggctg
    14101 gagcgatgtc ggctcctggt cctcattatg ggaaattagc gcccacaccg ccgagggcaa
    14161 cgtttgccac ggagatgtga ttaatcacaa aactgaaaac agctatgtgt acgccgaatc
    14221 tggcctggtc accaccgtcg gggtgaaaga tctggtggtg gtgcagacca aagatgcggt
    14281 gctgattgcc gaccgcaacg cggtgcagga tgtgaaaaaa gtggtcgagc agatcaaagc
    14341 cgatggtcgc catgagcatc gggtgcatcg cgaagtctat cgtccgtggg gcaaatatga
    14401 ctctatcgac gcgggcgacc gctaccaggt gaaacgcatc accgtgaaac cgggcgaagg
    14461 cttgtcggta cagatgcatc atcaccgcgc ggaacactgg gtggttgtcg cgggaacggc
    14521 aaaagtcact atcgatggtg atatcaaact gcttggtgaa aacgagtcga tttatattcc
    14581 gctgggggcg acgcactgcc tggaaaaccc ggggaaaatt ccgctcgatt taattgaagt
    14641 gcgctccggc tcttatctcg aagaggatga tgtggtgcgc ttcgcggatc gctacggacg
    14701 ggtgtaaacg tcgcatcagg cgctgtttgt cggatgcggc gtgaacgcct tatccgacct
    14761 acggttcggt tttgtaggcc tgataagacg cggcagcgtc gcatcaggca atgaatgcga
    14821 aaccgagatg taaataacga caaaaacaaa attggccgtt tcggtcaggg ccaactattg
    14881 cctgaaaaag ggtaacgaca tgaaaaaatt aacctgcttt aaagcctatg atattcgcgg
    14941 gaaattaggc gaagaactga atgaagatat cgcctggcgc attggtcgcg cctatggcga
    15001 atttctcaaa ccgaaaacca ttgtgttagg cggtgatgtc cgtctcacca gcgaaacctt
    15061 aaaactggcg ctggcgagag gtttacagga tgcgggcgtc gatgtgctgg atatcggcat
    15121 gtccggcacc gaagagatct atttcgccac gttccatctc ggcgtggatg gcggcattga
    15181 agttactgcc agccataatc cgatggatta caacggcatg aaactggtgc gcgaaggggc
    15241 tcgcccgatc agcggcgata ccggactgcg cgacgtccag cgtctggcag aagctaacga
    15301 cttccctcca gtcgatgaaa ccaaacgcgg tcgctatcag caaatcaatc tgcgtgacgc
    15361 ttacgttgat cacctgttcg gttatatcaa cgtcaaaaac ctcacgccgc tcaagctggt
    15421 gattaactcc gggaatggcg cagcgggtcc ggtggtggac gccattgaag cccgctttaa
    15481 agccctcggc gcgcccgtgg aattaatcaa agtacacaac acaccggacg gcaatttccc
    15541 caacggtatt cctaacccgc tgctgccgga atgtcgcgac gacacccgca atgcggtcat
    15601 caaacacggc gcggatatgg gcattgcctt tgatggcgat tttgaccgct gcttcctgtt
    15661 tgacgaaaaa gggcagttta ttgagggtta ctacattgtc ggcctgctgg cagaagcgtt
    15721 cctcgaaaaa aatcccggcg cgaagatcat ccacgatcct cgtctctcct ggaataccgt
    15781 tgatgtggtg actgccgcag gtggcacccc ggtaatgtcg aaaaccggac acgcctttat
    15841 taaagaacgt atgcgcaagg aagacgccat ctacggtggc gaaatgagcg cccaccatta
    15901 cttccgtgat ttcgcttact gcgacagcgg catgatccca tggctgctgg tcgccgaact
    15961 ggtgtgcctg aaaggaaaaa cgctgggcga actggtgcgc gaccggatgg cggcgtttcc
    16021 ggcaagcggt gagatcaaca gcaaactggc gcaacccgtt gaggcgatta accgcgtgga
    16081 acagcacttt agccgtgagg cgctggcggt ggagcgcacc gatggcatca gcatgacctt
    16141 tgccgactgg cgctttaatc tgcgctcctc caacaccgaa ccggtggtgc ggttgaatgt
    16201 ggaatcacgc ggtgatgtgc cgctgatgga agcgcgaacg cgaactctgc tgacgttgct
    16261 gaacgagtaa tgtcggatct tcccttaccc cactgcgggt aaggggctaa taacaggaac
    16321 aacgatgaca aatctaaaaa agcgcgagcg agcgaaaacc aatgcatcgt taatctctat
    16381 ggtgcaacgc ttttcagata tcaccatcat gtttgccgga ctatggctgg tttgcgaagt
    16441 cagcggactg tcattcctct acatgcacct gttggtggcg ctgattacgc tggtggtgtt
    16501 ccagatgctg ggcggcatca ccgattttta tcgctcatgg cgcggtgttc gggcagcgac
    16561 agaatttgcc ctgttgctac aaaactggac cttaagcgtg attttcagcg ccggactggt
    16621 ggcgttcaat aatgatttcg acacgcaact gaaaatctgg ctggcgtggt atgggctgac
    16681 cagtatcgga ctggtggttt gccgttcatg tattcgcatt ggggcgggct ggctgcgtaa
    16741 tcatggctat aacaagcgta tggtcgccgt agcgggggat ttagccgccg gacaaatgtt
    16801 gatggagagc ttccgtaatc agccgtggtt agggtttgaa gtggtgggcg tataccacga
    16861 cccaaaaccg ggcggcgttt ctaacgactg ggcgggcaat ctgcaacagc tggtcgagga
    16921 cgcgaaagcg ggcaagattc ataacgtcta tatcgcgatg cagatgtgcg atggcgcgcg
    16981 agtgaaaaaa ctggtccatc aactggcgga caccacctgt tcggtgctgc tgatccccga
    17041 tgtctttacc ttcaacattc tccattcacg tctcgaagag atgaacggcg ttccggtggt
    17101 gccgctttac gacacgccgc tttccggggt taaccgcctg ctaaaacgtg cggaagacat
    17161 tgtgctggcg acgcttattc tgctgctgat ctccccggtg ctgtgctgta ttgcgctggc
    17221 ggtgaaactc agttcaccag ggccggttat tttccgccag actcgctatg gcatggatgg
    17281 caaaccgatc aaagtgtgga agtttcgttc catgaaagtg atggagaacg acaaagtggt
    17341 gacccaggcg acgcagaacg atccgcgcgt caccaaagtg gggaactttc tgcgccgcac
    17401 ctcgctggat gaactgccgc agtttatcaa tgtgctgacc ggggggatgt cgattgtcgg
    17461 tccacgtccg cacgcagtag cgcataacga acagtatcgc cagctcattg aaggctacat
    17521 gctgcgccac aaagtgaaac cgggcattac cggctgggcg cagattaatg gctggcgcgg
    17581 cgaaaccgac acgctggaga aaatggaaaa acgcgtcgag ttcgaccttg agtacatccg
    17641 cgaatggagc gtctggttcg atatcaaaat cgttttcctg acggtattca aaggcttcgt
    17701 taacaaagcg gcatattgat atgagcttac gtgaaaaaac catcagcggc gcgaagtggt
    17761 cggcgattgc tacggtgatc atcatcggcc tcgggctggt acagatgacc gtgctggcgc
    17821 ggattatcga caaccaccag ttcggtttgc ttaccgtgtc gctggtgatc atcgcgctgg
    17881 cagatacgct ttctgacttc ggtatcgcta actcgattat tcagcgaaaa gaaatcagtc
    17941 accttgaact caccacgttg tactggctga acgtcgggct ggggatcgtg gtgtgcgtgg
    18001 cggtgttttt gttgagtgat ctcatcggcg acgtgctgaa taacccggac ctggcaccgt
    18061 tgattaaaac attgtcgctg gcgtttgtgg tgatccccca cgggcaacag ttccgcgcgt
    18121 tgatgcagaa ggagctggag ttcaacaaaa tcggcatgat cgaaaccagc gcggtgctgg
    18181 caggcttcac ttttacggtg gttagcgccc atttctggcc gctggcgatg accgcgatcc
    18241 tcggttatct ggtcaatagt gcggtgagaa cgctgctgtt tggctacttt ggtcgaaaaa
    18301 tttatcgccc cggtctgcat ttctcgctgg cgtcagttgc gccgaactta cgctttggtg
    18361 cctggctgac ggcggacagc atcatcaact atctcaacac caacctttca acgctggtgc
    18421 tggcgcgtat tctcggcgcg agcgtggcag gggggtacaa cctggcgtac aacgtggccg
    18481 ttgtgccacc gatgaagctg aacccaatca tcacccgtgt gttgtttccg gcgtttgcca
    18541 aaattcagga cgacaccgaa aagctgcgta ttaacttcta caaactgctg tcggtggtgg
    18601 ggattatcaa ctttccggcg ctgctggggc tgatggtggt gtcgaataac ttcgtaccgc
    18661 tggtattcgg cgaaaagtgg aacagcatta ttccggtgct gcaattgctg tgtattgtgg
    18721 gtctgctgcg ctccgtgggt aacccgattg gttcgctgct gatggcgaaa gcgcgggtcg
    18781 acatcagctt taaattcaac gtattcaaaa cctttctgtt tattccggcg attgttattg
    18841 gtggtcagat ggcgggcgca atcggcgtca cgctcggttt cctgctggtg cagattatca
    18901 acaccattct gagttacttc gtgatgatta aaccggttct tggttccagt tatcgccagt
    18961 acatcctgag tttgtggctg ccgttttacc tctcgctgcc gacgctggtg gttagttatg
    19021 cgctgggcat tgtgctgaaa gggcaactgg cgctggggat gctgctggcg gtgcaaatag
    19081 ccacgggtgt gctggcgttt gtggtgatga ttgtgctgtc gcgccatccg ctggtggtgg
    19141 aagtaaagcg tcagttttgt cgcagcgaaa aaatgaaaat gcttttacgg gcggggtgag
    19201 ttgttgtttt cggttccgac aacggttgcc gggtgcagcg taaaacgcct tatccatcct
    19261 acggttctgt gctcgggacc tgtaggcctg ataagacgcg tcagcgtcgc atcaggcact
    19321 gaatgccgga tgcggcgtga acgccttatc cggcctacgg ttctgtgctc gggacctgta
    19381 ggcctgataa gacgcgtcag cgtcgcatca ggcaaaaagc ggcaacgaac aaaacaaacg
    19441 cagcctcacc tggcatcaaa tttatgagga aaaaatgaaa ttacttattc tgggcaacca
    19501 cacttgcggc aaccgcggcg acagcgccat cctgcgcggc ttacttgatg ccatcaacat
    19561 cctcaatcca cacgccgaag tggacgtaat gagccgctat ccggtcagtt cttcctggct
    19621 gctcaaccgc ccggtaatgg gtgatccgct gttccttcaa atgaaacaac acaacagcgc
    19681 ggcgggcgtt gtcgggcgcg ttaaaaaagt cctccgtcgc cgctatcagc accaggtatt
    19741 gctctcacgc gtcaccgaca ctggcaagct gcgcaatatc gccatcgccc agggattcac
    19801 cgacttcgtg cgcctgctgt caggttacga cgccattatc caggtcggcg gatcgttttt
    19861 tgtcgatctc tacggcgtgc cgcagtttga acacgcgctt tgcacattta tggcgaaaaa
    19921 gccgctgttt atgattggtc acagcgtcgg ccccttccag gatgagcaat ttaaccaact
    19981 ggcgaactac gtttttggtc actgcgacgc gctgatcctg cgcgaatcgg tcagccttga
    20041 tctgatgaaa cacagcaata tcaccaccgc aaaagtggaa catggcgtcg ataccgcgtg
    20101 gctggtcgat caccacaccg aagacttcac cgccagctat gccgtccaac actggctgga
    20161 cgttgccgca caacagaaaa cggtggcaat taccctgcgc gaactggcac cgttcgacaa
    20221 acgcctcggc accactcagc aagcgtatga aaaagccttt gccggggtgg tcaatcgcat
    20281 tctcgacgaa gggtatcagg tgattgcgct ctccacctgt acgggcatcg acagctataa
    20341 caaagacgac cgcatggtgg cgctcaacct gcgccagcac atcagcgatc ctgcccgtta
    20401 tcacgtagtg atggatgaac tcaacgatct ggaaatgggc aaaattctcg gtgcctgtga
    20461 actcaccgtc ggtacgcgcc tgcactccgc cattatctcg atgaattttg ctactccggc
    20521 aattgccatc aactacgaac ataaatccgc cgggattatg caacaactgg ggctgccaga
    20581 gatggcaatt gatattcgtc atttattaga cggcagcctg caagcgatgg ttgcggatac
    20641 cttaggccag cttccggcgc tgaatgcgcg acttagtgaa gccgtcagtc gtgagcgtca
    20701 gacaggaatg cagatggtgc agtccgtact ggaacgcatc ggggaggtga aatgaaggtc
    20761 ggcttcttct tattgaaatt tccgctgtcg tcggaaacct tcgtcctcaa ccaaatcact
    20821 gcgtttattg atatgggctt tgaggtggag attgtcgcgc tgcaaaaagg cgacacacaa
    20881 aacacccacg cggcatggac gaaatacaac cttgccgcca gaacccgctg gttacaggac
    20941 gaacctacgg gcaaagtggc gaaactgcgc caccgagcca gtcagacctt gcgcggcatt
    21001 catcgtaaaa atacttggca ggcgctcaac ctcaaacgct atggtgccga gtcgcggaac
    21061 ctgattttgt ctgccatttg cggccaggtc gcaacaccat tttatgccga tgtctttatc
    21121 gctcattttg gccctgcggg ggtaaccgca gcaaaactac gcgaactggg tgtcattcgc
    21181 ggcaaaattg ccactatctt ccacggtatt gatatctcca gtcgggaagt gctcaaccac
    21241 tacactcccg aatatcaaca actgtttcgc cgtggcgacc tgatgttacc gataagcgat
    21301 ttgtgggccg gaaggctgca aaaaatgggc tgcccgaggg aaaaaattgc cgtatcgcgt
    21361 atgggcgtgg acatgacgcg ttttagcccg cgtccggtga aaccgcccgc aacgccgctg
    21421 gaaatcattt ccgtcgcacg cttaaccgag aaaaaaggcc tgcatgtggc gatcgaagcc
    21481 tgccgtcagt tgaaagagca gggcgtggca tttcgctatc gcatcctcgg cattggcccg
    21541 tgggaacgac gcctgcgcac gctcatcgaa caatatcaac tggaagatgt ggtggagatg
    21601 ccgggcttta aaccgagcca tgaagtgaaa gcgatgctcg acgatgcgga tgtcttcctc
    21661 ttgccatcgg ttacaggtgc ggacggcgat atggaaggca ttccggtggc gctgatggaa
    21721 gcgatggcgg tcggcattcc ggttgtttct actctacata gcggaatacc ggaactggtg
    21781 gaggccgata aatccggctg gctggtgcct gagaacgatg ctcgcgcact ggcgcaacgc
    21841 ctggcggcat ttagccaact ggacaccgac gaactggctc cggtcgtcaa acgtgcacgc
    21901 gaaaaagtcg aacacgattt taaccagcag gtgattaatc gagaactcgc cagcttgctg
    21961 caggctttat agaggagaac gtatgccatt taaaaaactc tcccgacgca ccttcctgac
    22021 ggcaagctcc gcgcttgcct tcctccatac acctttcgcc cgtgcacttc ctgcccggca
    22081 aagcgttaac attaacgact acaaccctca cgactggatc gcctcattta aacaagcctt
    22141 cagcgaaggg catacggtcg tcgtgcctgc cggattggtt tgtgacaata tcaacaccgg
    22201 catcttcatt ccttctggca aaacgttaca catccttgga agcctgcgcg gcaacggcag
    22261 agggcgattt gtcttacagg atggcagcca ggtgacaggg gaggagggcg gcagtatgca
    22321 taacatcacc ttggatgtgc gcggctctga ctgcaccatc aaagggctgg cgatgagcgg
    22381 ctttggcccg gtaacgcaga tttatattgg tggcaaaaac aaacgggtca tgcgcaacct
    22441 gaccatcgat aacctcactg tcagccacgc taattacgcc atcttacgcc agggatttca
    22501 taaccagatt atcggtgcca acatcaccaa ctgtaagttc agtgatttac agggcgacgc
    22561 cattgaatgg aacgtggcaa ttaacgacag tgatattttg atctctgacc atgtcatcga
    22621 gcgcatcaac tgtaccaacg gcaaaatcaa ctggggaatc ggcataggcc ttgcggggag
    22681 tacttatgat aataactacc cggaagacca ggcagtgaaa aactttgtcg tggcgaatat
    22741 cacgggatcg gattgtcggc agttgatcca tgttgaaaat ggtaaacatt ttgttattcg
    22801 taatatcaaa gcccgcaata tcacgccgga tttcagtaag aaagcaggta ttgataacgc
    22861 cacagtcgct atttacggtt gtgacaattt cgtgattgat aatattgaaa tgattaatag
    22921 cgccgggatg ttaatcggct atggggtaat taaaggcaaa tatctctcga taccacaaaa
    22981 tttccgagtg aataatattc agctggataa tacccacctt gcttataaat tgcgcggcat
    23041 ccaaatctcc gccgggaatg ctgtctcctt tgtggcactg actaacattg agatgaagcg
    23101 tgcgtcgctt gagctacaca ataaaccgca acaccttttt atgcgaaata tcaatgtgat
    23161 gcaggaatcc tcagttggac cagcattgag catgaacttc gacatgcgca aagacgttcg
    23221 cggcgtcttt atggcgaaaa aagaaacact gctgtctctt gcaaatgttc atgcggtgaa
    23281 tgaaagaggg caaagctccg tcgatatcga caggattaat caccatattg ttaatgtgga
    23341 aaagattaac tttagattgc cggaacggag ggagtagatt tgcgaccatt cctggaaaaa
    23401 tggagccata cttaggaaca atgctactgc aatccacaac gaagcggcgt aacatcacaa
    23461 gtaattcagt aatcaattca gggtaattga tgctggcgaa aaaaatcgaa caagctataa
    23521 ttcagcaacc attttacagg tggatgaaat aatgacgaat ttaaaagcag ttattcctgt
    23581 agcgggtctt gggatgcata tgttgcctgc cactaaggcg attcccaaag agatgctacc
    23641 gatcgtcgac aagccaatga ttcagtatat tgttgacgag attgtggctg cagggatcaa
    23701 agaaatcctc ctggtaactc atgcgtccaa gaacgcggtc gaaaaccact tcgacacctc
    23761 ttatgaatta gaatctcttc ttgagcagcg cgtgaagcgt cagctgctgg cggaagttca
    23821 gtccatctgc ccgccgggcg tgaccattat gaacgtacgt cagggcgaac ctttaggttt
    23881 aggtcactcc attttgtgtg cacgacccgc cattggtgac aacccatttg tcgtggtgct
    23941 gccagacgtt gtgatcgacg acgccagtgc cgatccgctg cgttacaacc ttgctgccat
    24001 gattgcgcgt ttcaacgaaa cgggccgcag ccaggtgctg gcaaaacgta tgccgggtga
    24061 cctctctgaa tactccgtca ttcagaccaa agaaccgctg gatcgcgaag gcaaagtcag
    24121 ccgcattgtt gaatttatcg aaaaaccgga tcagccgcag acgctggact cagacatcat
    24181 ggccgttggt cgctatgtgc tttctgcaga tatttggccg gaacttgaac gcactcagcc
    24241 aggtgcatgg ggacgtattc agctgactga tgccattgcc gaactggcga aaaaacagtc
    24301 tgttgatgcc atgctgatga caggtgacag ctatgactgc ggtaaaaaaa tgggttatat
    24361 gcaggcattt gtgaagtatg gactacgcaa cctgaaagaa ggggcgaagt tccgtaaagg
    24421 gattgagaag ctgttaagcg aataatgaaa atctgaccgg atgtaacggt tgataaggaa
    24481 attataacgg cagtgaagat tcgtggcgaa agtaatttgt tgcgaatatt cctgccgttg
    24541 ttttatataa acaatcagaa taacaacgag ttagcaatag gattttagtc aaagttttcc
    24601 aggattttcc ttgtttccag agcggattgg taagacaatt agcgtttgaa tttttcgagt
    24661 taagcgcgag tgggtaacgc tcgtcacatc gtagacatgc atgcagtgct ctggtagctg
    24721 taaagccagg ggcggtagcg tgttttattt actgcatgta tttgagaaaa gtagacatga
    24781 taaatattaa tatgcgtaaa a
//
LOCUS       DAZKIV010000062        23904 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZKIV010000062 DAZKIV010000000
VERSION     DAZKIV010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23904
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.062"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..3862)
                     /gene="entF"
                     /locus_tag="ACHOT6_002411"
     CDS             complement(<1..3862)
                     /gene="entF"
                     /locus_tag="ACHOT6_002411"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFP9947615.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHVDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQVQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPII"
     gene            complement(3859..4077)
                     /gene="ybdZ"
                     /locus_tag="ACHOT6_002412"
     CDS             complement(3859..4077)
                     /gene="ybdZ"
                     /locus_tag="ACHOT6_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFP9947616.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCPQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(4080..5282)
                     /gene="fes"
                     /locus_tag="ACHOT6_002413"
     CDS             complement(4080..5282)
                     /gene="fes"
                     /locus_tag="ACHOT6_002413"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFP9947617.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5525..7765
                     /gene="fepA"
                     /locus_tag="ACHOT6_002414"
     CDS             5525..7765
                     /gene="fepA"
                     /locus_tag="ACHOT6_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFP9947618.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            7805..7903
                     /locus_tag="ACHOT6_002415"
                     /pseudo
     CDS             7805..7903
                     /locus_tag="ACHOT6_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7940..8560
                     /gene="entD"
                     /locus_tag="ACHOT6_002416"
     CDS             7940..8560
                     /gene="entD"
                     /locus_tag="ACHOT6_002416"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFP9947619.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(8682..8834)
                     /gene="hokE"
                     /locus_tag="ACHOT6_002417"
     CDS             complement(8682..8834)
                     /gene="hokE"
                     /locus_tag="ACHOT6_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9947620.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(9185..9337)
                     /gene="hokE"
                     /locus_tag="ACHOT6_002418"
     CDS             complement(9185..9337)
                     /gene="hokE"
                     /locus_tag="ACHOT6_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFP9947621.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            9306..9575
                     /locus_tag="ACHOT6_002419"
     CDS             9306..9575
                     /locus_tag="ACHOT6_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947622.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            9790..10908
                     /gene="ybdK"
                     /locus_tag="ACHOT6_002420"
     CDS             9790..10908
                     /gene="ybdK"
                     /locus_tag="ACHOT6_002420"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFP9947623.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            10974..11222
                     /gene="ybdJ"
                     /locus_tag="ACHOT6_002421"
     CDS             10974..11222
                     /gene="ybdJ"
                     /locus_tag="ACHOT6_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFP9947624.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            11287..11655
                     /gene="ybdF"
                     /locus_tag="ACHOT6_002422"
     CDS             11287..11655
                     /gene="ybdF"
                     /locus_tag="ACHOT6_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9947625.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            11749..12402
                     /gene="nfsB"
                     /locus_tag="ACHOT6_002423"
     CDS             11749..12402
                     /gene="nfsB"
                     /locus_tag="ACHOT6_002423"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFP9947626.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            12510..13757
                     /gene="ybdG"
                     /locus_tag="ACHOT6_002424"
     CDS             12510..13757
                     /gene="ybdG"
                     /locus_tag="ACHOT6_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFP9947627.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(13825..15201)
                     /gene="pheP"
                     /locus_tag="ACHOT6_002425"
     CDS             complement(13825..15201)
                     /gene="pheP"
                     /locus_tag="ACHOT6_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFP9947628.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(15303..18446)
                     /gene="cusA"
                     /locus_tag="ACHOT6_002426"
     CDS             complement(15303..18446)
                     /gene="cusA"
                     /locus_tag="ACHOT6_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFP9947629.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTFDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIASVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(18458..19681)
                     /gene="cusB"
                     /locus_tag="ACHOT6_002427"
     CDS             complement(18458..19681)
                     /gene="cusB"
                     /locus_tag="ACHOT6_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFP9947630.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(19697..20029)
                     /gene="cusF"
                     /locus_tag="ACHOT6_002428"
     CDS             complement(19697..20029)
                     /gene="cusF"
                     /locus_tag="ACHOT6_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFP9947631.1"
                     /translation="MKKALQVAMFSLLTVIGFNAQANEHHHETMSEAQPQVISATGEV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(20053..21435)
                     /gene="cusC"
                     /locus_tag="ACHOT6_002429"
     CDS             complement(20053..21435)
                     /gene="cusC"
                     /locus_tag="ACHOT6_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFP9947632.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIHQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            21592..22275
                     /gene="cusR"
                     /locus_tag="ACHOT6_002430"
     CDS             21592..22275
                     /gene="cusR"
                     /locus_tag="ACHOT6_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFP9947633.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            22265..23713
                     /gene="cusS"
                     /locus_tag="ACHOT6_002431"
     CDS             22265..23713
                     /gene="cusS"
                     /locus_tag="ACHOT6_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFP9947634.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         5763 a         6525 c         6059 g         5557 t
ORIGIN      
        1 gaataatcgg cccaattttt acaaacgccc ctggagagat aatatccaca tgcgcacaat
       61 cctgacgata gatatccagc tccgcaatcc acggcgacca agcacgttcg ggactcatac
      121 cttcctgaag tgtgcgctca gcaacaaata acgttgcttt tccatcaaac ggaacgctat
      181 gggcggtggt taacaagcgc acagcatcag cgtagttgcc ttcaatggtg gtaaacaact
      241 ccgttgaagt acttccctgc tgtgccgcca ggaaggcctc gcgttcgcgg ttaatctccg
      301 ccagcacttc cgggtccaga ccattagcct ctttttcctg ccagttttgc gtctctggcg
      361 gccaggtatc cagcaaacca agaaatgcca cctgttcgcc acgggcacgc agtcgcgccg
      421 caataccctg cgccagcgta ccgccaaggg aataccccag caggtaataa gggccgtgcg
      481 gttgttgctc aagtaacgtt gccagatgcg cttcgcagac ttcatccagg tttgtcgctg
      541 tctgcatggg tccatgaggg cgcggcgact gaatgccaat aatcgaccat tgtggatcga
      601 gataacgcga gagcacgcta aactgccagg caaaaccgga cgccggatgg aaacaaaaca
      661 gcgtcgggcc attaccttca cgcaacggca gaatggtttc gaatcccatg cgccgggagc
      721 tgtcctcttc accatcaata atcgttgcca gtttggcgac ggttgacgcg accatcacct
      781 gccccggcgt cacctgacga gcaaactgtc gacttaactg cgctgccagt ttcattgcca
      841 gtagcgaatg accgcccagc gcaaagaaat cagcatcggc atcctgcacg tcacaaccca
      901 gcaacgacgc gaatgccgcg gcgataatcg tttcactgcc cgctttcggt gcacgtcccg
      961 acgcttgtgt cttcagttca ggcaacggta aggctttgcg atccagcttg ccgttggcgc
     1021 taagcggcaa ctgcggaagt tgcagcagaa cgaccggcac catatgcggc ggcaatgttt
     1081 cgcgaagctg tacctgtaat gcgctggtat ccaacggcag tcccgattgc gacaccagat
     1141 agcccaccaa ctgacgcgca tcaccaccgg tggctgccgc ctggttaatc acacaggcgt
     1201 gggtaactgc ctgttcgaca tccggcagcg cctgcatcac gcgatcgatt tcgcccagtt
     1261 cgatacgctg cccgcgaatt tttagctgat cgtcactgcg cccgaggtac tccaccgcgc
     1321 cgttatccag ccagcgggcg acgtctccgg tacgatacat ccgttcacct ggggcaaaag
     1381 gatcggcaat aaaacggctg gcggtcagat cgggtcgtcc aagatacccc tgcgccagtt
     1441 ggatgccggt gagatagaga tcacccgcca cacccggcgg caccggatgc atcatcgcat
     1501 caagaatacg cagacccgta ttccataccg gataaccaat cggcacactg ctgccgcgca
     1561 cctgtgccag ttcctcgcca aaagccggat accagctgac atctaccgcc gcttccgtcg
     1621 ggccatacag gttatgcaac ggcgcgccgg ttaactgttg ccattcgcgg cataaatcag
     1681 ccggtaaggc ctcaccacta cagaaaacct gtttcaacgt cgcgcaattc tggcgagcgg
     1741 tttgcggcgt cagcgatgca acaaatgccg ccagcatcga cggcacaaag tgcgtggtcg
     1801 ttacgccata ttcggcaaag aattgctgca tagcgagcgg gtcacggtgc gcttccggtt
     1861 cagccatcac cagttttgcc cctgcgataa acggccagaa aaactcccac accgagacat
     1921 caaaactgca cggcgttttt tgggcaacga catcttcgcc tgtaagcggg tagtgatttt
     1981 gcatccataa caggcggtta acgatagccg tctgcccgac cattacccct ttcggcctgc
     2041 cggtggagcc agaggtaaag atgatataag ccgtgtggtg cggttgtgaa agttgcagcg
     2101 gcgcactgcc ctggggggta agcggggcgt tatagcaaag gcttgttaaa ttgggaacat
     2161 cgctaaagcg cggcagttga tcgtcggtgg taattaacag cgacggacgc gcatcttcca
     2221 gcatcatttt caggcgatcg tccggatagc cggtatccag cggtagccag gccgcgccag
     2281 cttcaactat tgcatggagt gccagggtca aaaagaccga gcgcggtagt gccaccgcca
     2341 cgctgtcccc tggtttaacg ccgcgctcac gcagcagatt cgccagcgcc accacctgct
     2401 cgcgcatttc ccgatagctg aacaggtaac gcgcatctgc cagcgccgga gcatccggtg
     2461 tttttgccgc ttgttctgcc accagcgcgc tcagcgtggt ttctggaatc tcaacctgag
     2521 tggcgttgat ctgcgccagc tgcgcatact cacctggcag cataatatcg acatcgccgc
     2581 acaacagcgc cggatccgcg gcgaactggg caatcagcat tttcaggcgt tcagcatgct
     2641 ggattaacgt tggctcatcg taacgctgtt tattggcgag gatctcaata ctcaaatcaa
     2701 cgtgttcatc cgggaacagg gccagttcaa ggtcattaac cggaccggtt gccagggtat
     2761 gggtttgcgt ctgaacacca ggaatatcca gttggtaatc aaataccttg atattgagta
     2821 ccggaccgaa cagcggttcc tctcctgccg ctcgcccgct gtcacggaca atttgttcgg
     2881 catcgtaacg ttgatgacga cgcatttttt tcagttgtgc tgccagtcgg gttgccagtt
     2941 ccggcagcgt ttcttgcgcc gcaatgtgaa tacccaacgg taaaacgttg agcacgggtc
     3001 cgctagccgt cagcgccgcc gagcccagtc gacgcataaa gataaatccg gcggcatagt
     3061 ccatgcgatt gcacaatcgc cccagccaca aggctgccag cgcaagggct aaatcggtac
     3121 gctgcacacc tgaaagttgc gtagccagct ggcggaattc cccgtcggta aattccagtt
     3181 tcaggcgcag aatatctgcc gaagcgctgc gccccgctaa aggtgccgga gaaagtgacg
     3241 cgggcggcgg cagttgacga cgctgttctg cccagaatgc cgcatcacgc tgccaggctt
     3301 cgctttcgcg gtattgctgg tactcttcca ctacatcagc gaaaggcgta aacggcgaag
     3361 caggcgttgg ttcgccacgc agccatgtgc agtaaatatt ggcgatctgg cgagtaatgg
     3421 ccgggaaact gaagccatcg accagcaaat ggtgataacg ctgataccag taccagcggt
     3481 tatccgcaac atgtatcagc tgatgaaaga ccagtggttt accgctatcg acgcgcaaat
     3541 cttgttgcaa atccgccagc attaatgcct gcgcagtacc gtgcggatca atattggttc
     3601 gcaggtcgat aatttctggc agttcgaata tcagcgcatc atcgacccac tgccagactt
     3661 cgccgttatc ttccgtaaaa cgcatccgca acgtatctgc ttgcgctagt ccggcaacca
     3721 ccgcgcgggc cagtaatggc gcatcaacat ctccagttaa ctcaacgtaa tgcgccacgc
     3781 tccaggcgga gggtaaatcc gacagttttt ctgccatcca gatgccgggc tgtgcggcga
     3841 ccaaaggtaa atgctggctc attgtgcctc ctgcaactgg gtgaaattgg ccggtgtcag
     3901 agtacgccag tggacttcca gccactgcgg gcaggacgcc tgtgactgcg gctgacacac
     3961 aatgtcccag cctgccggta aggcgcattg ttgcggccac agactgaatt gcccctgcgc
     4021 attgcgcaaa atgtaaaacg ctccctgtgg atcatcgaag ggattactga atgccatatt
     4081 caactcctgt catggaaaag tggttgccag aggtcgatta gcccctgcat caagccaccg
     4141 cgccaacaaa gcgcatcatg tccgccgtca acctgacgcc agaaaatgga ttcttttatg
     4201 gggtgtaatt gcgcatacag cgcctgattg gcccgcataa tcatcggctc gcgaataccc
     4261 gcttccagca caatgcgcag accttcggcg ctaacttcac cagctttaag cttttcaagt
     4321 aacacgccct cttgctgccc gccccgatgc ggccaccagt acgatcctga ctggcttaat
     4381 acacagccaa agcgttcagg ccagtgcagt ccggcataca gcgcggacag cccaccaaaa
     4441 ctctgcccgg cgaccacggt gcgatcggcg cgatcgctaa aaggggcaat agctttcacc
     4501 aggggtaata actcttgctg tactgccagc cagaaatccg cattacacgg cagttcgtgg
     4561 gcgcggtgcg tggtgtcgat agcgtcgatc aacacataca cggcgggagg aagttgctga
     4621 cgatgggtca gcgaagtcag cacaggccag acgggcatac tttgcgccca aaattcgcca
     4681 tcgagcaaaa ctgccagcgg acgttcttct gctgttgcat cgccggtggt aaaaatccat
     4741 acacgacgtg aatttttcaa ccgttcactt ttccagataa tttctttggc tgctatttct
     4801 ggcgcttgcg gacaatccca tcccggttgc agaggcgctt gcggcatttc gagtgcagaa
     4861 acagcgtgcc ctcgcccgcc tttccagctt tgtggattca gcggatcggc tattgcctgg
     4921 ggtaatagtt ttcgccatcc ttcacgtaat tcgaggcgat cgggggatgg tgcagaaaaa
     4981 atgtcatcgc gttcggtggg aataaagcag tagctgccgc gccagttggc attgagttgt
     5041 gtcgtccact gccagacatc agtacctgca attcgctgca tcgactgggg ctggctgttc
     5101 tgatgatgat cggtcacacc agtgatgtag atccatacgc gctttatcgt cgagtattct
     5161 tcagaacctt ggggatcacg ccaccagaaa gtgacctcaa acatttcgtc attcagacgc
     5221 tgccattccg ggccatgttt cgactgccac cagctctcac ttcctacttt taacgccgtc
     5281 acaccataac cccatgttta ctgtgcaatt tttcattgat tgcagaaata tattgataat
     5341 attattgata accatttgca tttgcaatag cgtaatggcg cgccgtggga agcgcggaca
     5401 tttattaacc aactgcactg cgtgtctttc aggatcaaag gttttcgcgg tagcgggatg
     5461 cgtcgtgttg aggacgacca tgcccgacag ttgcaattcg tggcaaaaat gcaggaataa
     5521 aacaatgaac aagaagattc attccctggc cttgttggtc aatctgggga tttatggggt
     5581 agcgcaggca caagagccga ccgatactcc tgtttcacat gacgatacca ttgtcgttac
     5641 cgccgccgag cagaacttac aggcgcctgg cgtttcgacc atcaccgcag atgaaatccg
     5701 caaaaacccc gttgcccgcg atgtgtcgga gatcatccgt accatgccag gcgttaacct
     5761 gaccggtaac tccaccagtg gtcagcgtgg gaataaccga cagattgata ttcgcggtat
     5821 gggtccggaa aacacgctga ttttgattga cggcaagccg gtaagcagcc gtaactcggt
     5881 gcgtcagggc tggcgtggcg agcgcgatac ccgtggtgat acctcctggg tgccacctga
     5941 aatgattgaa cgtattgaag ttctgcgtgg tccggcagct gcgcgttatg gcaacggcgc
     6001 ggcgggcggc gtggttaaca tcattaccaa aaaaggcagc ggtgagtggc acggctcctg
     6061 ggacgcttat ttcaatgcac cagaacataa agaggaaggt gccactaaac gcaccaactt
     6121 cagcctgacc ggtccgctgg gcgacgaatt cagcttccgc ttgtatggca acctcgacaa
     6181 aacccaggct gacgcgtggg atatcaacca gggccatcag tccgcgcgtg ccggaacgta
     6241 tgccacgacg ttaccagccg ggcgcgaagg ggtgatcaat aaagatatta acggcgtggt
     6301 gcgctgggac ttcgcaccat tgcaatcgct ggaactggaa gcgggttaca gccgccaggg
     6361 taacctgtat gcgggcgata cccagaacac caactctgac gcttacactc gctcgaaata
     6421 tggcgatgaa accaaccgcc tgtatcgcca gaactacgcg ctgacctgga acggtggctg
     6481 ggataacggc gtgaccacca gcaactgggt gcagtatgaa cacacccgta actcgcgtat
     6541 tccggaaggt ctggcgggcg gtaccgaagg gaaatttaac gaaaaagcga cacaggattt
     6601 cgtcgatatc gatctcgatg acgtgatgct gcacagcgaa gttaacctgc cgattgattt
     6661 cctcgttaac cagacgctga cgctgggtac ggagtggaat cagcaacgga tgaaggactt
     6721 gagttccaat acccaggcgc tgaccggggc gaacaccggc ggcgctattg atggtgtgag
     6781 tgccaccgac cgtagcccgt attcaaaagc agaaattttc tcgctgtttg ccgaaaacaa
     6841 catggagctg actgacagca ccatcgtaac gccggggctg cgtttcgatc atcacagtat
     6901 tgtcggcaat aactggagcc cggcgctgaa catatcgcaa ggtttaggcg atgacttcac
     6961 gctcaaaatg ggcattgccc gtgcttataa agcaccgagc ctgtaccaga ctaacccgaa
     7021 ctacattctc tacagtaaag gtcagggctg ctatgccagc gcgggcggct gctatctgca
     7081 aggtaacgat gacctgaaag cagaaaccag catcaacaaa gagattggtc tggagttcaa
     7141 acgcgacggc tggctggcgg gcgtcacctg gttccgtaac gattatcgca ataagattga
     7201 agcgggctat gtggctgtag ggcaaaacgc agtcggcacc gatctctatc agtgggataa
     7261 cgtgccgaaa gcagtggttg aaggtctgga aggatcgtta aacgtaccag ttagcgaaac
     7321 ggtgatgtgg accaataaca tcacttatat gctgaagagt gaaaacaaaa ccacgggcga
     7381 ccgtttgtcg atcatcccgg agtacacgtt gaactcaacg ctgagctggc aggcgcggga
     7441 agatttgtcg atgcaaacga ccttcacctg gtacggtaag cagcagccga aaaaatacaa
     7501 ctacaaaggt cagccagcgg ttggcccgga aaccaaagaa atcagtcctt acagcattgt
     7561 gggtctgagc gcgacctggg atgtgacgaa gaatgtcagt ctgaccggcg gcgtggacaa
     7621 cctgttcgac aaacgtttgt ggcgtgcggg taatgcccag accacgggcg atctggcagg
     7681 ggccaactat atcgcgggtg ccggggcgta tacctataac gagccgggac gtacgtggta
     7741 catgagcgta aacactcact tctgatgcta acgtcagatt gttgacaaag tgcgcgtcgt
     7801 tcatgccgga tgcggcgtga acgccttatc cggcctacaa aaatcttgcc aattcaatat
     7861 attgcaggac catgtaggcc tgataagcgt agcgcatcag gcagtttagc gtttgtcatc
     7921 agtctctaat atggtcgaca tgaaaactac gcatacctcc ctcccctttg ccggacatac
     7981 gctgcatttt gttgagttcg atccggcgaa tttttgtgag caggatttac tctggctgcc
     8041 gcattacgcg caactgcaac acgctggacg gaagcgcaaa gccgaacatt tagccgggcg
     8101 gatcgctgca atttatgcgc tgcgggaata tggctataaa tgtgtgcccg caatcggcga
     8161 gctacgccaa ccggtgtggc ctgcggaggt atacggcagt attagccact gcggggctac
     8221 ggcattggcc gtggtttccc gtcaaccgat tggcgttgat atcgaagaaa ttttttctgc
     8281 gcaaacagcg accgaattga cagacaacat tattacacca gcagaacacg agcgactcgc
     8341 agactgcggt ttagcctttt ctctggcgct gacactggca ttttccgcca aagagagcgc
     8401 atttaaggca agtgagatcc aaactgatgc aggttttctg gactatcaga taattagctg
     8461 gaataaacag caggtcatca ttcatcgtga gaatgagatg tttgctgtgc actggcagat
     8521 aaaagaaaaa atagtcataa ctctgtgcca acacgattaa ttgacaacat ctggtacgat
     8581 tcgcccgcag ccatcactga ccgcgggcga aagtgtaaag caggtgcctt accactctga
     8641 cctgacaacc ggatatgcgg ggattgctcc ccgcacaacg gctacttctt cggttcgtaa
     8701 gcgagaacag ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgagtct
     8761 ccgaccagaa gcgtaaatcc cagtaccgtc aaacacagca ctatgactgc cacaagggca
     8821 tatttcgtca gcatgtttat attgcctcca tgcgaagaag ggactaccat ccgagttgtt
     8881 gaggctcgga gtggcagccc caggattgat agaaatatct cctggggctt catctttcca
     8941 gacctcagag ttaacctgaa accggaaagc ttcaggcacc cgccgttatc ttacccttcc
     9001 tcaacaaaat agctgtttat ttatacagtg aatcctgttt cggacttctg tcggcaaaac
     9061 tcatcattgc tcttaaaacg aaatcgcccg cagccatcac tgaccgcggg cgaaagtgta
     9121 aagcaggtgc cttaccatcc tgacctgaca accggatatg cggggattgc tccccgcaca
     9181 acggctactt cttcggttcg taagcgagaa cagccttaaa ctcaatatta cgttccttta
     9241 cggtgaactc acacagcgaa tccccgacca gaagcgtaaa tcccagcacc gttaaacaca
     9301 gcactatgac tgccgcaagg gcatatttcg tcagcatgtt tatattgcct ccatgcgaag
     9361 aagggactac catccaaatt gttcaggttt gggtaggtag ccccagggtt gatagaaata
     9421 tcgtcctggg gcttcatctt tccagacctc agagtaaacc tgaaaccgga aagcttcagg
     9481 cacccgcagt tatcttaccc ttgcttagca aaatagctgt ttatttatac agtaagtcct
     9541 gtttcggact tctcactccc catttgatca actgaagctg aacccgtttt tttctgcatg
     9601 cctcaatcgt caaaattctt cgccaccagt cagcactgcc ataacttgtc acattgcaaa
     9661 agtagccaaa ttgcttctgt cataatcagt tcaccatcac caaaaaaaga aacattccag
     9721 aacttccgca tctcgcccag ccgctcccac ttctatactg aatagaaaac gccaacataa
     9781 gagaaaccta tgccattacc cgattttcat gtttctgaac cttttacact cggtattgaa
     9841 ctggaaatgc aggtggttaa tccgccgggc tatgacttaa gccaggactc ttcaatgctg
     9901 attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa agcacgatat caccgaaagt
     9961 atgctggagc tggcgacgga tgtttgccgt gatatcaacc aggctgccgg gcaattttca
    10021 gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc atctggaaat ttgcggcggt
    10081 ggcacacacc cgtttcagaa atggcagcga caggaggtat gcgataacga gcgctatcaa
    10141 cgaacgctgg aaaactttgg ttatctcatt caacaggcga ccgtttttgg tcagcatgtt
    10201 catgttggct gcgccagtgg cgatgacgcc atttatttgc tgcacggcct gtcacggttt
    10261 gtgccgcact tcatcgccct ttccgccgcg tcgccatata tgcaaggaac ggatacgcgt
    10321 tttgcctcct cacgaccgaa tattttttcc gcctttcccg ataatggccc gatgccgtgg
    10381 gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt tgagttacac cacgatgatc
    10441 gacagcatta aagatctgca ctgggatatt cgccccagtc ctcattttgg cacggtggag
    10501 gttcgggtga tggatacccc gttaaccctt agccacgcgg taaatatggc gggattaatt
    10561 caggctaccg cccactggtt actgacagaa cgcccgttca aacatcagga gaaagattac
    10621 ctgctgtata aattcaaccg tttccaagcc tgtcgctatg ggctggaagg cgtcattacc
    10681 gatccgcaca ctggcgatcg tcgaccgcta acggaagaca ccttgcgatt gctggaaaaa
    10741 atcgcccctt ccgcacataa aattggtgca tcgagcgcga ttgaggccct gcatcgccag
    10801 gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt tcgtcgccga tggtggctcg
    10861 ctgattgggc tggtgaaaaa gcattgtgag atctgggccg gtgactaagt agggaattgc
    10921 cttacagaag aatatccctg acaatggtgt tttaacttac atttaacaat gttatgaaac
    10981 accctttaga aaccttgact accgcagcag gcattttgct gatggctttc ctctcttgcc
    11041 tgctgctgcc cgcccccgca ctggggctga cgctggcaca aaaactggtg accatgttcc
    11101 atctgatgga tcttagccag ctttacactt tattgttttg tctgtggttt ttagtgctgg
    11161 gcgctattga gtattttgtt ctgcgcttta tctggcgacg ctggttctcg ctggcggatt
    11221 aaacgtcgat tatcgccgca cttcgcggca tactttgctt attctccttc gcctaaagga
    11281 acgtttatgg ataagcaatc actgcacgaa acggcgaaac gcctggccct tgagttaccc
    11341 tttgtcgagc tttgctggcc ttttggcccg gagttcgatg tttttaaaat tggcggcaaa
    11401 atttttatgc tgtcgtcgga gctgcgcggc gtccccttta tcaatctgaa gtccgatcca
    11461 caaaaatccc tgttaaatca gcagatatac ccgagcatta agccagggta tcacatgaat
    11521 aaaaagcact ggatttcggt gtatcccggc gaggaaatct ccgaagcatt acttcgcgat
    11581 ctgatcaacg attcgtggaa tctggtggtt gatggtctgg ctaaacgcga tcaaaaaaga
    11641 gtgcgtccag gctaaagcgg aaatctatag cgcatttttc tcgcttacca tttctcgttg
    11701 aaccttgtaa tctgctggca cgcaaaatta ctttcacatg gagtctttat ggatatcatt
    11761 tctgtcgcct taaagcgtca ttccactaag gcatttgatg ccagcaaaaa acttaccccg
    11821 gaacaggccg agcagatcaa aactctcctg caatacagcc catccagcac caactcccag
    11881 ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag cgcgtgttgc caaatccgct
    11941 gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg cctcgcacgt cgtggtgttc
    12001 tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg ttgttgacca ggaagatgct
    12061 gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg ataaaggtcg caagttcttc
    12121 gctgatatgc accgtaaaga tctgcatgat gatgcagagt ggatggcaaa acaggtttat
    12181 ctcaacgtcg gtaacttcct gctcggcgtg gcggcgctgg gtctggacgc ggtacccatc
    12241 gaaggttttg acgccgccat cctcgatgca gaatttggtc tgaaagagaa aggctacacc
    12301 agtctggtgg ttgttccggt aggtcatcac agcgttgaag attttaacgc tacgctgccg
    12361 aaatcacgtc tgccgcaaaa catcacctta accgaagtgt aattctctct tgccgggcat
    12421 ctgcccggct atttcccctc agattctcct gatttgcata accctgtttc agccgtcatc
    12481 ataggctgct gttgtataaa ggagacgtta tgcaggattt aatatcccag gttgaagatt
    12541 tagcgggtat tgagatcgat cacaccacct cgatggtgat gattttcggt attatttttc
    12601 tgaccgccgt cgtggtgcat attattttgc attgggtggt actgcggacc ttcgaaaaac
    12661 gtgccatcgc cagttcacgg ctttggttgc aaatcattac ccagaataaa ctcttccacc
    12721 gtttagcttt taccctgcag gggattatcg tcaatattca ggcggtattc tggctgcaaa
    12781 aaggcaccga agcggcagat attctgacta cctgcgcgca gttgtggatc atgatgtatg
    12841 cgctgctttc agtcttctcg ttgctggatg tgattttgaa tctggcgcag aaattcccgg
    12901 cggcatctca gttaccgctg aaagggatat ttcaggggat taaactgatc ggcgcgattc
    12961 tggtcggcat tttgatgatc tcgctgctga ttggtcagtc gccagcgatt ctgatcagcg
    13021 gtcttggtgc aatggctgcc gtgctgatgt tggtttttaa agatccgatt cttggtctgg
    13081 tggcaggtat tcagctttcc gcgaacgata tgctgaaact gggcgactgg ctggagatgc
    13141 cgaaatacgg cgcggatggc gcggtgatcg atattgggtt aaccaccgtc aaagtgcgta
    13201 actgggacaa taccattacc actattccca cctggtctct ggtttctgac tcctttaaaa
    13261 actggagcgg gatgtcagca tctggcgggc gacgtattaa gcgcagtatc agtattgatg
    13321 tcaccagtat tcgttttctt gatgaagacg aaatgcaacg tctgaataaa gcgcacttgt
    13381 taaaacctta tttaaccagc cgccatcagg aaattaatga gtggaatcgc cagcaaggtt
    13441 ctacggagtc gatattaaac ctgcgccgaa tgaccaatat tggaaccttt cgtgcctatc
    13501 tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga tatgacttta atggtacgcc
    13561 aactggctcc aggtgataac ggtttaccgc tcgagatcta tgcgtttacc aacaccgtgg
    13621 tgtggctgga atatgaaagc attcaggctg atatattcga tcacatattt gcgattgtcg
    13681 aagagtttgg tctgcgactt catcagtcgc caaccggcaa tgatattcgc tctctggcgg
    13741 gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa tggatgagag cggacatcgt
    13801 tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc gttttaaatg ccacaaacag
    13861 gaatacaatc cacaccggca gcaggatcgc tgacaagcgc atatcatcca tcgtgcacat
    13921 cagcagcaaa atcatgccga ggaaggcaat gcaaagatag ttgccgaacg gatagagcag
    13981 cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct gcacgaaaac gcagatgcgc
    14041 caggcagatc ataatccagt tcaacagcag cgttgctacc accagcgcca tcagcagacc
    14101 aaacgctttt tgcggcagca gatagttgat taataccacc agcgaagtga tcgctccgga
    14161 aagcatcagc gaattaatcg gcacaccgcg acggctgacg cgagtcaaaa acttcggcgc
    14221 attaccctgc acagaaaggc caaacagcat gcggctgtta gagtaaaccc cgctgttata
    14281 cactgacagc gatgctacca gaatgacgaa gttcagcgca gaagctacca cgttgctgtc
    14341 gagattatgg aaaatcatca caaacgggct actgttggat ttcacttcca cccacggata
    14401 gagcgccagt aaaaccacca gtgagccgat gtaaaacagc aggatgcgat acaccacctg
    14461 atttaccgct tttggaatgc ttttttccgg atcgcgcgct tcagcggcag taatcccaat
    14521 cagctccaga ccgccgaagg agaacataat taccgccagc gacaaaatca gcccattcca
    14581 gccggtggca aagaaaccac cgtagcgcca gaggttgtcg atactggctt tctcgccgcc
    14641 gtgaccagaa aacagcagcc acaggccaaa gccgatcata ccgatgatcg ccagcacttt
    14701 aatcagcgca aaccagaact cggtttcacc atataagcgc acgttcacca ggttaacggc
    14761 gttgatgata ataaagaagg cggcagccca aatccacgtt ggaacatccg ggaaccagta
    14821 ctgcatatag atgcccgcag cggtcagctc tgccattccc accagcacga acattaccca
    14881 gtagttccag ccagagagga agcccgcaaa cggtccccag tatttatagg caaagtgggc
    14941 aaatgaaccg gataccggct cttcgaccac catctcgcca agctggcgca taatcaggaa
    15001 agcgatgatc ccggcgacgc cgtagcccag caatacagcc ggacccgcca tctgaatcgc
    15061 cggaccaatg ccaagaaaca gaccagtacc aattgcgcca cccagcgcaa tcagttgaat
    15121 atgacggtta tgtaatccgc gatgaagcgt cggctcttga ttcgacgcag tatcttccga
    15181 tacggttgac gcgtttttca cgcctttccc ctgtgtgtct tttttgttga ggggcacctt
    15241 ttaacattta gtgcccatcg tcgcaagaca caatccacac ggttaaacgg ggtatcctgc
    15301 ttttatttcc gtacccgatg tcggtgcagc cacatcagct tatacgccgc cgggataata
    15361 aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca tcggtgcggc aatccggctc
    15421 atcacctctg aaccagcccc cgttccccac agaatcggca gcagaccggc gataatcacc
    15481 gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc cgtgatacaa cgcctcatcc
    15541 agcttctgct cactgaatgt ttgcggatta ttcaacgacg gctcggcctc tatggcgtga
    15601 cgtaaataca tcagcatcac cacgccaaat tcggcggcga ccccggcaag ggcaataaaa
    15661 ccggtgcccg ttgccacaga aagatgaaag cccatccacc acagcaacca gatgccgccc
    15721 accagcgcaa acggtacgct gctgataatc agcaacgctt cgcccacccg acggaatgcc
    15781 agatataaca gcacgaagat gatcatcagt gtcatcggca ccatgagttt cagcttatgg
    15841 ttggcgcgct ccagtagctc gaactgcccg gagaatgcca cgctggtgcc cggttttaac
    15901 tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca ccgacaccat gtcacgatcg
    15961 cgggcatcga tataaatcca gctcgtcggg cgcgcattct cggttttcag catcgacggt
    16021 ccggtagaga ctttaacgtc ggccacgtct gccagggtga tttgctgctt catcggcgtc
    16081 aggatcggca actggcgcag tgcctgcgga ctatcgcgcc agctttgcgg ataacgcaga
    16141 ttaatgggat aacgggcaat cccttccacc gtttcgccaa ccatcgcccc gcccaccgca
    16201 gaagtcacaa acaactgcac atccgccacc gtcataccgt aacgcgcggc tttttcacgg
    16261 ttaatctcaa cgttgatata gcgtccacct tccagacgct cggcaagcgc agaagccacg
    16321 cctggtaccg ttcgtgccac ctcttcaatt tgctccgcca tcgcgtcgat atccgccagc
    16381 acagtgccgg aaactttaat gccgatgggg cttttaatgc cagtcgagag catatcgata
    16441 cggttacgaa ttggcggcac ccacagattc gccagccccg gcagacgcac ggtgttatcc
    16501 agttcctcaa tgattttgtc catcgtcatg cctggccgcc actgctcctg cggcttaagc
    16561 tggatggtcg tttctaccat ctccagcgga gcagaatcgg tggcggtttc cgctttcccg
    16621 gttttgccaa atacccgcgc cacttcaggt acgctcataa ttagcttgtc ggttttttgc
    16681 agcatactcg ccgcctctgc tgcggaaatc cccggcagcg tcgatggcat atacaacaag
    16741 tcgccttcat tgatctgcgg taaaaattcc ccgccaactt tattgagcgg ccagagaacc
    16801 gtcagcaccg aaagcgccgc caccagcagc gtggttttcg gccagtgcag tactttcagc
    16861 aacagcggat gataaacacg aatcaaaaag cgattgagcg ggttactgct ttccggcgga
    16921 attttgccac ggatccagta gcccatcagg atcgggatca ctacgatcgc cagcagcgcc
    16981 gcacccgcca tcgcatacgt tttggtgaac gccaacgggc caaacagacg cccttcctgc
    17041 ccttccaggg tgaagatcgg gataaacgac aacgtgataa tcagcagact gataaatagc
    17101 gccggcccca cttcaacaga cgcatcggtg atcacctgcc agcgcgtttt attatccagc
    17161 gtggcgtcag gatgctggtg ctgccactct tccagccgtt tatgcgcatt ctcgatcatg
    17221 acgatagcag catcgaccat cgccccgacg gcaatcgcaa tgccacccaa cgacataata
    17281 ttggcattca gtccctggaa gtgcatgaca ataaaagcaa tacacaaccc cagcggcaac
    17341 gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa acagcgcaca gactaccgcc
    17401 accacaataa actcttccag caacttgccg ctgaggttgt cgatggcgcg gtcaatgagc
    17461 tggctgcgat cgtatgttgt aactatctcc acgccttccg gcagactact tttcagcgtt
    17521 tccagtttgt ccttcacgga ggcgatcact tctcgggcgt ttttgccgga gcgcaggatc
    17581 accaccccgc ccgccacttc accttcgcca ttcagttcgg caatgccccg gcgcatctcc
    17641 gggccaatct ggaccttcgc aacatcacgc agataaacgg gcacgccatt ttcactggct
    17701 tttaaaacga tgtgattaaa gtcgtcgaac gtttgcagat agccgctggc gcgcaccata
    17761 tattccgctt ccgccagttc gatcgacgaa ccgcccgctt cctggtttga agcatccagc
    17821 gcgcttttta cttcggctag actgatgcca tattgcgcca ggcgctgggg atcgataacc
    17881 acctgatact ctttcaccac gccgcccacc gacgccactt ccgcaacgtc agggatggtt
    17941 tttagctcat atttgagaaa ccagtcctgc aatgagcgta aatcggccag atcgtgctta
    18001 ccgctgcgat ccaccagtgc atattcatag atccagccaa cacccgtggc atctggtccc
    18061 agctcggcgc tgactcccgc aggcagctta ccctgtacct ggttgaggta ctccagcacc
    18121 cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa tgacatacac ataagagtct
    18181 ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca ccgacaacat ggtggtggtt
    18241 agcggataag tcacctgatt ttcaacgatt tgcggtgcct gaccgggata gctggtttta
    18301 ataatcacct gcacatcgga gagatccggc agcgcatcca ctggcgtgtt aatgatggtc
    18361 caggtgcccc agatgctcag aaacaacgcg cccatcagca ccaaaaaacg gttcgccacc
    18421 gagcgacgaa taatccattc aatcattggt tattccctca atgcgcattg gtagcacttt
    18481 cagagcgcat ccgctccagt gcgccagaaa tattggcttc ggaatcaatc aggaacaggc
    18541 cgctggaaac caccttttca ccttccgcca gaccagagtg taacgcggtg acgccttgcg
    18601 atgcctggaa aacggcaacg cgtttcggta caaagcgtcc gtcggcatca acggtaatca
    18661 cccgctgttc gttgccggta tcaatcagcg cctgtgacgg aatgagcagc atcggttcgc
    18721 tggcggtgtt gagttgcaac caggcgttca tgcccggttt tagcgcctcg tcggcgttgt
    18781 cgacttccag acgcagctgc agcgtgcggg tcgcggcatc cacgccaggt agcagcgtcc
    18841 atttgcggat ggtgagtgtt ttatccggtc gcgccggaac ggtgagcgta aactgcgagg
    18901 catctttcac cagccaggcg atagactccg ggatcgcagc agtgacccac accgggtcca
    18961 taccctgaat tttcgctacc acgttatctt tggcgatatt cattcccgcg cgcagatcaa
    19021 acgcggtgat cacgccatca atgggtgctt tgagcgtaaa gcgagtctgg attttttgcg
    19081 tggcgatcag gcggcgaata tccgcctccg gcattcccgc cagtcgcagc cgctcaagaa
    19141 tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag cagtaaatac tcactctgcg
    19201 cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt gcccttttgt actttatcgc
    19261 ccacggtaag cggatacacc ttgtcgataa acccggcagc gcgagcctgc acaatggcat
    19321 actgatactc gttgtaactg acattcgccg ggaaactctg ggcaaaagtc agcggcccgc
    19381 gcgtgacggt agccgttttc acccccagat tctgcgtctg agtcgggtca atgcgcacac
    19441 cagacgcaga actctcttca tcggcatatt tcggcaccag atccatatcc ataaacggcg
    19501 atttacctgg tttatcgaac cgcgtattgg gatacatcgg gtcgtaccag aataagattt
    19561 tacgttctgc ggtcgacgtt ttttctgcgg gtggttccgc ctttgcaaac caggtaaaac
    19621 ctgccgcaga aataataccg cccgcgatca tgctgccgat aataagcgcg atttttttca
    19681 tctcattaaa cctgggttac tggctgactt taatatcctg taataaagaa aggttgccct
    19741 gctggacaaa attaaacgcc actttgtcgc cagttttaat ttcactcatt ttcgtctgcg
    19801 gagtgatggt aaagcgcatg gtcatctccg gccagttcac ggcagcaatc ggatcgtgat
    19861 ggatggtgat ttttttgctt tccagatcaa taccctttac ctcgccagtg gcgctaataa
    19921 cctgtggttg tgcttcgctc atggtttcat gatgatgttc gttagcctgg gcattaaagc
    19981 caataacggt taacagactg aacattgcta cttgcagtgc ttttttcatt tattctctcc
    20041 tggagttaaa agttattgct gccaaccgcc accaagtgcg gtatataaag aaatttcgtt
    20101 aacctgacgg gcataattca gatcaagtaa agtttgtcgg gttgcaaata aagaacgctc
    20161 ggcatccagc acttccagat aacttactgc gccgtgctga tataatgccc gcgcccgttg
    20221 caaagtaatt tgcagcgacg ccagataacg ttgctgggcg ctaatttgat cgttcaggct
    20281 ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg ttctggattt tctgttcata
    20341 attcaccacc gactgctgct ggtgaatttc ggcgatatcc agattggcct ggttgcgtcc
    20401 ggcattaaaa atggggatct caattttagg aataaaattc cacatcccgc tgctagcgtt
    20461 aaataatgaa gacagatcgc tgctggcggt tgatattccg ctggtcaggc tgatagacgg
    20521 gaaaaatgcc gcacgcgcag cgccaatatt ggcattagcc gccattaacg cgtgttcagc
    20581 ttccataata tccgggcgct gcaataagat ttgcgacgac aatcccgccg gtaatttaac
    20641 gctttgcagg ctgtcgctgt ttactgtctg cgcttgcggc agcttgccgt agcttcccaa
    20701 taacagttgc aatgcattat tcgcctgcgc cagttccccc tgacgtttag cgatgtcgct
    20761 gcgggtactt tcaatcaccc cgcgagcctg ttccagcgcc agaacattac tgctacctgt
    20821 caacagttgt ttttcgacaa acgcatatga ctgctgataa ttacgcagcg tttcttcggc
    20881 tatttgcaat tgcgcatacg ctagctgctg attgaaataa ctttgcgcga cattagaaac
    20941 cagcaaaata tgcaccgcgc gctgggcttc ctcagtggct aaataatttt ggcgctcggc
    21001 ttcgctcatg ttctttaagc gaccgaaaaa atcgagatca aagctggcgt taaggccggt
    21061 cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta ccgctccagc tgccgctgcc
    21121 ctcgccattg agctgtgggt agcggtcggc atcggtcaga cgatattgcg cccgcgcttc
    21181 ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc accagcgcct cgctaatcag
    21241 cgtctttacc tgattatcaa caaaaaaggt gcgccagccc acgttctggt agttatctgc
    21301 tgcgttaacc aagccgttct ggctgagtga gaactgctgc ggcacgggca ttgccggacg
    21361 ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg gccacacaaa atggcagaag
    21421 tttacaagga gacataggct cataatttct ggtgatttta taccgccaac tttactcgcc
    21481 aggctctgat tttccggtga caggaaaatg acaaaattgt cattttgcca ctaagcgatt
    21541 gccatctgat cccgctactc tagaattgcc cgggcaacat gcggaggaaa aatgaaactg
    21601 ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga ccaaagggtt aaccgaagcc
    21661 ggttttgtgg tcgatttggc cgacaacggg ttgaatggct accatctggc gatgaccggt
    21721 gattatgatc tgataatcct cgatattatg ctgccggacg tgaacggctg ggatatcgtg
    21781 cgcatgttgc gttccgccaa taaagggatg ccgattctgt tgcttaccgc gcttggcacc
    21841 attgaacatc gcgtcaaggg gctggagttg ggggccgatg actatctggt gaagccgttc
    21901 gcttttgctg aactgctggc gcgggtgcgc actctcctgc ggcgcggggc ggcggtgatt
    21961 atcgaaagtc agtttcaggt tgccgatctg atggtcgatc tcgtcagccg caaagtcacc
    22021 cgcagcggca cgcgcatcac tctgaccagt aaagagttta ctctgctgga gttttttctc
    22081 cgccatcagg gcgaagtgct gccccgctcg cttatcgcct cgcaggtgtg ggacatgaat
    22141 ttcgacagcg acactaacgc cattgatgta gcggtgaagc ggctgcgcgg caaaatcgac
    22201 aacgactttg agccgaagct aattcagacc gtgcgcggcg tgggctacat gcttgaggtg
    22261 ccggatggtc agtaagccat ttcagcgccc gttttccctg gcaacccgcc tgaccttttt
    22321 tatcagcctg gccaccatcg cggcgttttt cgcctttgca tggatcatga tccactcagt
    22381 aaaagtgcat tttgccgagc aggatattaa tgatttaaaa gagattagcg ccacgcttga
    22441 acgggtactt aatcaccctg acgaaacgca agcccgacgc ttaatgacgc tggaagatat
    22501 cgtcagtggt tattccaacg tgttgatttc cctggcagat agccacggca aaacggtgta
    22561 tcactccccc ggtgcgcctg atatccgcga gtttgcacgt gatgccatac ccgataaaga
    22621 cgcgcggggt ggcgaggtgt atctcctttc cggcccgacg attatgatgc caggccacgg
    22681 tcacgggcat atggaacaca gcaactggcg gatgattaac ttgtcggttg gcccgttggt
    22741 ggacggcaaa ccgatttata cgctctacat cgcgctttcg atcgattttc atcttcatta
    22801 cataaatgat ttgatgaata aacttattat gaccgcatcg gtaatcagca tcctgatcgt
    22861 ctttatcgta ctgttggcgg tacataaagg tcacgcgccg atccgcagcg tcagccgtca
    22921 aatccagaat atcacctcga aagatctcga cgttcgcctt gacccgcaga ccgtgcccat
    22981 tgagctggaa cagctggtac tgtcgttcaa ccatatgatc gagcgtattg aggatgtctt
    23041 tacccgccag tccaatttct cagcggatat cgctcacgaa attcgcacgc cgattaccaa
    23101 tctcatcacg caaacggaaa tcgccctcag ccagtctcgc agccagaagg agctggaaga
    23161 tgtgctctac tctaatctcg aagagctgac gcgaatggcg aaaatggtca gcgatatgct
    23221 gtttctcgct caggccgata acaaccagct aatccccgaa aagaaaatgc tcaacctggc
    23281 ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta gcggaagatc gcggcgtgga
    23341 gttgcgattt gttggcgacg aatgtcaggt tgcgggcgat ccgctgatgc tgcgtcgggc
    23401 gttaagcaac ctgctttcta atgccctgcg ttatacgccg cccggagagg caattgtagt
    23461 gcgctgccag acggtcgatc accaggtgca agttaccgtc gaaaaccccg gtacgcccat
    23521 tgcgcccgag cacttaccgc gattgtttga ccgtttctac cgcgttgacc cttcccgcca
    23581 gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg aaatcgattg ttgtcgcgca
    23641 taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca aggtttgtga ttatgctgcc
    23701 ggagagagag tgattattaa gttcaaattc agggcgtggt gcatcacctc ctggtattgt
    23761 tgatcaacaa attagagaaa aacttattaa acatatgaaa tatcgaaggc ttttattttt
    23821 caaagtttca cgagatgttt gtatatgaaa cttagttaaa tctgtgtttc ttccaattgt
    23881 agatcacaat gccctttcta aatt
//
LOCUS       DAZKIV010000063        23870 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZKIV010000063 DAZKIV010000000
VERSION     DAZKIV010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23870)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23870)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23870
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.063"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1646
                     /gene="gcl"
                     /locus_tag="ACHOT6_002432"
     CDS             <1..1646
                     /gene="gcl"
                     /locus_tag="ACHOT6_002432"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFP9947635.1"
                     /translation="LARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSA
                     SADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFH
                     LMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRP
                     VIVAGGGVINADAAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYG
                     NATLLASDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDA
                     KAALTLLVEVAQEMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMN
                     KAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPER
                     KVVAISGDFDFQFLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAF
                     ENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVI
                     LERVTNISMGSELDNVMEFEDIADNAADAPTETCFMHYE"
     gene            1659..2435
                     /gene="hyi"
                     /locus_tag="ACHOT6_002433"
     CDS             1659..2435
                     /gene="hyi"
                     /locus_tag="ACHOT6_002433"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFP9947636.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR"
     gene            2535..3413
                     /gene="glxR"
                     /locus_tag="ACHOT6_002434"
     CDS             2535..3413
                     /gene="glxR"
                     /locus_tag="ACHOT6_002434"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9947637.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            3582..5036
                     /locus_tag="ACHOT6_002435"
     CDS             3582..5036
                     /locus_tag="ACHOT6_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFP9947638.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGKINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            5096..6457
                     /gene="allB"
                     /locus_tag="ACHOT6_002436"
                     /pseudo
     CDS             5096..6457
                     /gene="allB"
                     /locus_tag="ACHOT6_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            6514..7815
                     /locus_tag="ACHOT6_002437"
     CDS             6514..7815
                     /locus_tag="ACHOT6_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFP9947639.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            7837..8982
                     /gene="glxK"
                     /locus_tag="ACHOT6_002438"
     CDS             7837..8982
                     /gene="glxK"
                     /locus_tag="ACHOT6_002438"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFP9947640.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDVNYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(9210..9996)
                     /gene="allE"
                     /locus_tag="ACHOT6_002439"
                     /pseudo
     CDS             complement(9210..9996)
                     /gene="allE"
                     /locus_tag="ACHOT6_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
     gene            complement(10007..11242)
                     /gene="allC"
                     /locus_tag="ACHOT6_002440"
     CDS             complement(10007..11242)
                     /gene="allC"
                     /locus_tag="ACHOT6_002440"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFP9947641.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSVEKAKRMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            complement(11264..12313)
                     /gene="allD"
                     /locus_tag="ACHOT6_002441"
     CDS             complement(11264..12313)
                     /gene="allD"
                     /locus_tag="ACHOT6_002441"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFP9947642.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12630..14297
                     /locus_tag="ACHOT6_002442"
     CDS             12630..14297
                     /locus_tag="ACHOT6_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFP9947643.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQAL
                     TQVRRWDSACQKLPDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIAQAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAIAPVSSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERAPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIYPLSPAAQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            14307..15566
                     /locus_tag="ACHOT6_002443"
     CDS             14307..15566
                     /locus_tag="ACHOT6_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFP9947644.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            15577..16392
                     /locus_tag="ACHOT6_002444"
     CDS             15577..16392
                     /locus_tag="ACHOT6_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFP9947645.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSYPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            16389..17279
                     /locus_tag="ACHOT6_002445"
     CDS             16389..17279
                     /locus_tag="ACHOT6_002445"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFP9947646.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGLV
                     ILTDADAVYENWGTPQQRAIRHATPDELAPFSKVDGSMGPKVTAVSGYVRSRGKPAWI
                     GALSRIEETLAGEAGTCISL"
     gene            complement(17416..18483)
                     /gene="purK"
                     /locus_tag="ACHOT6_002446"
     CDS             complement(17416..18483)
                     /gene="purK"
                     /locus_tag="ACHOT6_002446"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFP9947647.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(18480..18989)
                     /gene="purE"
                     /locus_tag="ACHOT6_002447"
     CDS             complement(18480..18989)
                     /gene="purE"
                     /locus_tag="ACHOT6_002447"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFP9947648.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            complement(19107..19829)
                     /gene="lpxH"
                     /locus_tag="ACHOT6_002448"
     CDS             complement(19107..19829)
                     /gene="lpxH"
                     /locus_tag="ACHOT6_002448"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFP9947649.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            complement(19832..20326)
                     /gene="ppiB"
                     /locus_tag="ACHOT6_002449"
     CDS             complement(19832..20326)
                     /gene="ppiB"
                     /locus_tag="ACHOT6_002449"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFP9947650.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            20500..21885
                     /gene="cysS"
                     /locus_tag="ACHOT6_002450"
     CDS             20500..21885
                     /gene="cysS"
                     /locus_tag="ACHOT6_002450"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFP9947651.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            complement(21921..22442)
                     /locus_tag="ACHOT6_002451"
     CDS             complement(21921..22442)
                     /locus_tag="ACHOT6_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFP9947652.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(22550..22762)
                     /gene="ybcJ"
                     /locus_tag="ACHOT6_002452"
     CDS             complement(22550..22762)
                     /gene="ybcJ"
                     /locus_tag="ACHOT6_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFP9947653.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            complement(22764..23630)
                     /gene="folD"
                     /locus_tag="ACHOT6_002453"
     CDS             complement(22764..23630)
                     /gene="folD"
                     /locus_tag="ACHOT6_002453"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFP9947654.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
BASE COUNT         5542 a         5926 c         6551 g         5851 t
ORIGIN      
        1 ttctggcgcg tcatgtggaa ggtgcttcgc acatggcgga aggttatacc cgcgcaacgg
       61 cagggaatat cggcgtatgt ctggggactt ccggtcctgc gggcacggac atgatcaccg
      121 cgctctattc tgcttctgct gattccattc ctattctgtg tattaccggc caggctccgc
      181 gcgcccgtct gcataaagaa gatttccagg ctgttgatat cgaagctatc gccaagccgg
      241 tcagcaaaat ggcggttaca gtacgtgaag cggcgctggt gcctcgtgtg ctgcaacagg
      301 catttcacct gatgcgttct ggtcgtccgg gtccggtact ggtggattta ccgttcgacg
      361 ttcaggttgc ggaaatcgag tttgatcctg atatgtacga accgctgccg gtctacaaac
      421 ctgctgccag ccgtatgcag atcgaaaaag ctgtagaaat gttaatccag gccaaacgtc
      481 cggtgattgt tgccgggggc ggggtaatta atgctgacgc agctgcactg ttacaacagt
      541 ttgctgaact gaccagcgtt ccggtgatcc caacgctgat gggctggggc tgtatcccgg
      601 acgatcatga actgatggcc gggatggtgg gtctgcaaac cgcgcatcgt tacggtaacg
      661 caacgttgct ggcgtccgac atggtgtttg gtatcggtaa ccgttttgct aaccgtcata
      721 ccggttcggt agagaaatac accgaagggc gcaaaatcgt tcatatcgat attgagccga
      781 cgcaaattgg ccgcgtgctg tgtccggatc tggggattgt ctctgatgct aaagcggcgc
      841 tgacactgct ggttgaagtg gcgcaggaaa tgcaaaaagc agggcgtctg ccatgccgta
      901 aagagtgggt tgctgagtgc cagcagcgca aacgtacttt gttgcgtaaa acacacttcg
      961 acaacgttcc ggttaaaccg cagcgtgtgt atgaagagat gaacaaagct tttggacgtg
     1021 atgtttgcta tgtcaccacc attggtctgt cgcaaattgc cgctgcgcaa atgctgcatg
     1081 tctttaaaga ccgccactgg atcaactgtg ggcaggcagg cccgctgggc tggaccattc
     1141 cggcggcgct ggggggttgt gccgctgatc cagagcgcaa agtggtggcg atttccggtg
     1201 acttcgactt ccagttcctg attgaagaat tggccgttgg cgcacagttc aacattccgt
     1261 acatccatgt actggtcaat aacgcttatc tggggctgat tcgccagtcg cagcgcgcgt
     1321 ttgatatgga ctactgcgtg caactcgctt tcgagaatat caactccagt gaagtgaatg
     1381 gctacggtgt tgaccacgta aaagtagcgg aaggtttagg ttgtaaagcg attcgcgtct
     1441 tcaaaccgga agatattgcg ccagcctttg aacaggcgaa agccttaatg gcgcaatatc
     1501 gggtaccggt agtcgtggaa gttattctcg agcgtgtgac caatatttcg atgggcagcg
     1561 aactggataa cgtcatggaa tttgaagata tcgccgataa cgcagcggac gcaccgactg
     1621 aaacctgctt catgcactat gaataaggga gataaataat gttacgtttc tctgctaatt
     1681 tatcgatgtt atttggagaa tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg
     1741 gttttcgcgg cgttgaattt atgtttcctt atgactacga cattgaagaa ttaaaacagg
     1801 tgctggcgag taataaactc gaacatacgc tgcacaattt accggcgggt gactgggcgg
     1861 cgggggagcg cggtattgcc tgtattcctg gccgtgaaga agagtttcgg gatggcgtag
     1921 cagcagcgat tcgttatgcc cgtgcgctgg gtaataaaaa aattaactgt ctggtcggta
     1981 aaacgccggc tggtttcagc agtgaacaga ttcacgcaac gcttgtagaa aacctgcgtt
     2041 atgccgcgaa tatgctgatg aaagaagata ttttattact gattgaacct attaaccatt
     2101 ttgatattcc tggtttccat ctcaccggaa ctcggcaggc gctgaaattg attgatgatg
     2161 ttggttgctg caatttgaaa attcagtatg acatttatca catgcagcgg atggaaggtg
     2221 aattaaccaa caccatgact cagtgggctg ataaaattgg tcacctgcaa attgccgata
     2281 atccgcatcg cggcgaaccg ggaaccggag aaattaatta tgattatctc tttaaggtaa
     2341 tcgaaaattc tgactacaac ggttgggttg ggtgtgaata taaaccccaa accaccacgg
     2401 aagccggttt acgctggatg gatccgtacc gttaaaacgt aacgctattc agacaatgct
     2461 tttttaggcc gctaagttgg caggggatcg tgttgtctga attcaggaaa agcgaaattt
     2521 aaaagaggtt aattatgaaa ctgggattta ttggcttagg cattatgggt acaccgatgg
     2581 ccattaatct ggcgcgtgcc ggtcatcaat tacatgtcac gaccattgga ccggttgctg
     2641 atgaattact gtcactgggt gccgtcagtg ttgaaactgc tcgccaggta acggaagcat
     2701 cggacatcat ttttattatg gtgccggaca cacctcaggt tgaagaagtt ctgttcggtg
     2761 aaaatggttg taccaaagcc tcgctgaagg gcaaaaccat tgttgatatg agctccattt
     2821 ccccgattga aactaagcgt ttcgctcgtc aggtgaatga actgggcggc gattatctcg
     2881 atgcgccagt ctccggcggt gaaatcggtg cgcgtgaagg gacgttgtcg attatggttg
     2941 gcggtgatga agcggtattt gaacgtgtta aaccgctgtt tgaactgctc ggtaaaaata
     3001 tcaccctcgt gggcggtaac ggcgatggtc aaacctgcaa agtggcaaat cagattattg
     3061 tggcgctcaa tattgaagcg gtttctgaag ccctgctatt tgcttcaaaa gccggtgcag
     3121 acccggtacg tgtgcgccag gcgctgatgg gcggctttgc ttcctcacgt attctggaag
     3181 ttcacggtga gcgcatgatt aaacgcacct ttaatccagg tttcaaaatt gcgctgcacc
     3241 agaaagatct caacctggca ctgcaaagtg cgaaagcact tgcgctgaat ctgccaaaca
     3301 ccgcgacctg ccaggagtta tttaatacct gtgcggcaaa cggtggcagc cagttggatc
     3361 actctgcgtt agtgcaggcg ctggaattaa tggcgaatca taaactggcc tgatacccgc
     3421 aataaaaatg gccgatatca gaatatgaat cggccagcaa tattaaaaaa gaaagcagcc
     3481 aaagatgttg cttcagtatt aaaaataata tttttatttt atttgttcct catagctaga
     3541 ttaaaacaac gttattcgat acgtgaaatt aagagggatt tatggaacat cagagaaaac
     3601 tattccagca acgcggctat agcgaagatc tattgccgaa aacgcaaagc cagcggacct
     3661 ggaaaacatt taactatttt accttatgga tgggttcggt tcataacgtt cccaattatg
     3721 tgatggtcgg cggctttttt attctcggct tgtctacctt tagtattatg ttggcaatta
     3781 tcctcagcgc ctttttcatt gccgcggtaa tggtattaaa cggtgctgcg ggcagtaaat
     3841 acggcgtacc gtttgccatg atcctgcgtg cttcttacgg cgtacgtgga gcactgtttc
     3901 caggattatt aagaggcggt attgctgcca ttatgtggtt tggcctgcaa tgttacgcgg
     3961 ggtcactggc ctgcttgatt ctgattggca aaatctggcc gggattttta actctcggtg
     4021 gtgatttcac gctgttaggg ctttctctac cgggcttaat tactttctta ctcttctggc
     4081 tggtcaacgt tggtataggt tttggcggtg gcaaagtttt aaataaattc actgccattc
     4141 ttaacccgtg catctatatc gttttcggcg gtatggcgat ttgggcgatt tcgctggtcg
     4201 ggctcggtcc aatctttgac tacattccga gcggtattca gaaagcagaa aacagtggct
     4261 tcctgttcct ggtggtgata aacgcggtag ttgcggtctg ggcggcaccg gcggtgagcg
     4321 catccgactt tacgcaaaac gcccactcgt ttcgtgagca agcgctgggg caaacgctgg
     4381 gtttagttgt ggcctatatt ctgtttgcgg tcgcgggggt atgtattatt gccggagcca
     4441 gtattcatta cggcgctgat acctggaacg tgctggatat tgttcagcgt tgggacagct
     4501 tgttcgcctc gttctttgcg gtactggtta ttctgatgac gactatctcc actaacgcca
     4561 ccggtaatat tattcctgcc ggttatcaga ttgccgctat tgcaccgacg aaactgacct
     4621 ataaaaacgg cgtactgatt gccagtatta tcagcctgct gatctgcccg tggaaattaa
     4681 tggaaaatca ggacagtatt tatctgttcc tcgatattat cggcggaatg cttggtccgg
     4741 taattggtgt catgatggcg cattattttg tggtgatgcg tgggaaaatt aatcttgatg
     4801 aactgtatac cgcacctggc gattataaat attacgataa cggttttaac ctcactgcgt
     4861 tttcagtaac tctggtggcc gttattttat ctcttggcgg taagtttatt ccctttatgg
     4921 aaccgttatc gcgtgtttca tggtttgtcg gcgtcatcgt cgcctttgcg gcctacgcct
     4981 tattaaagaa acgtacaaca gcagaaaaaa caggagagca aaaaaccata ggttaattaa
     5041 tcccgatatt gaacattgag ttaaaaacca atctgtattt tacaaggagt ttgttatgtc
     5101 ttttgattta atcattaaaa acggcaccgt tattttagaa aacgaagctc gcgttgtaga
     5161 tatcgccgtt aaaggcggga aaattgctgc tatcggtcag gatctgggcg atgcaaaaga
     5221 agttatggat gcgtctggtc tggtggtttc gccgggcatg gttgatgcgc acacccatat
     5281 ttctgaaccg ggtcgtagcc actgggaagg ttatgaaacc ggtactcgcg cagcggcaaa
     5341 aggtggtatc accaccatga tcgaaatgcc gctcaaccag ctgcctgcaa cggttgaccg
     5401 cgcttcaatt gaactgaagt tcgatgccgc ttaaggcaag ctgactatcg atgcggcaca
     5461 actcggtggc ctggtgtctt acaacatcga ccgtctgcat gagctggatg aagtgggcgt
     5521 tgtcggcttc aaatgcttcg ttgcgacctg tggcgatcgc ggtatcgaca acgacttccg
     5581 tgatgtaaac gactggcagt tcttcaaagg tgcgcagaag ctgggcgaac tggggcagcc
     5641 ggtgctggtg cactgcgaaa acgcgctgat ctgtgacgca ctgggcgaag aagcgaaacg
     5701 tgaaggtcgc gtaactgccc atgactatgt ggcttcgcgt ccggtattta ccgaagtgga
     5761 agcgattcgc cgcgtgctgt atctggcgaa agttgccggt tgccgtctgc acgtttgcca
     5821 tatcagcagc ccggaaggcg ttgaagaagt gactcgtgca cgtcaggaag gtcaggacgt
     5881 tacttgtgaa tcctgcccgc attactttgt actggatacc gatcagttcg aagaaatcgg
     5941 cactctggcg aagtgttcac cgccgatccg cgatctggaa aaccagaaag gcatgtggga
     6001 aaaactgttt aacggtgaaa tcgactgcct ggtttccgac cactcaccat gccctccgga
     6061 aatgaaagcc ggcaacatca tggaagcatg gggcggtatt gccggtctgc aaaactgtat
     6121 ggacgtgatg ttcgatgaag cggtacagaa acgcggaatg tctctgccaa tgttcggcaa
     6181 attaatggcg actaacgcag cagatatttt cggtctgcag caaaaaggcc gtatcgcccc
     6241 aggaaaagat gccgacttcg tcttcattca gccgaatagc agctatgttc ttaccaatga
     6301 cgatctggaa tatcgccaca aagtcagccc gtatgttggc cgtaccattg gcgcgcgtat
     6361 cacgaaaacc atcttacgtg gtgatgtgat ttacgacatt gaacagggct tccctgttgc
     6421 gccgaaaggt caatttatcc ttaaacatca gcagtaatct ggcccctgca atgcccgtcc
     6481 ttgcggcggg cattctccgg ttaaggtgtg tttatgttca attttgcagt cagccgcgaa
     6541 agcctgttat caggatttca gtggtttttc tttatttttt gcaacacggt tgtggttcct
     6601 cctacgctac tttctgcttt tcagttgccg caaagtagcc tgcttacgct cacgcaatat
     6661 gcttttcttg ctaccgcact ggcctgcttc gctcaggcgt tctgcggtca tcgtcgcgct
     6721 attatggaag ggccgggtgg cctgtggtgg ggaaccatcc ttactatcac ccttggcgaa
     6781 gcatcgcgcg ggacaccgat caacgatatc gctaccagcc tggcggtggg gattgcgctc
     6841 tccggcgtgc tgacgatgtt gattggtttt agcggattag gccatcgcct ggcacggtta
     6901 tttacgccgt cggtgatggt cttgtttatg ttgatgctgg gcgcgcagct gaccactatc
     6961 tttttcaaag gtatgctcgg gctgccgttt ggcatagccg acccaaattt taaaattcag
     7021 ttaccgccgt tcgcgctctc ggtggcggtg atgtgcctgg tactggggat gattatcttc
     7081 ctgccgcaac gttttgcccg ttatggcctg ctggtcggca ccataaccgg ctggttgttg
     7141 tggtactttt gctttccttc ttcgcactcg ctctccggtg agttgcactg gcagtggttc
     7201 ccgctcggca gtggaggtgc tttgtctccg ggaattattc tgacggcggt gattacaggt
     7261 ctggtgaata tcagcaatac ctacggtgcg attcggggca cggatgtttt ttatccgcag
     7321 cagggcgcgg gaaatacgcg ttatcgtcgc agctttgtgg cgaccggatt tatgacgctg
     7381 ataaccgtac cgctggcggt aattccattt tcaccgtttg tttcatccat tggtttatta
     7441 acccagacag gcgattacac gcggcgttcg tttatttatg gcagcgttat ttgcctgctg
     7501 gtggcgctgg ttccggcact cacacgactg ttttgcagta tccctttacc cgtgagtagc
     7561 gcggtcatgc tggtttctta tctgccttta ctcttttccg cgctggtgtt tagccagcaa
     7621 ataaccttta ccgctcgcaa tatttatcga ctcgcattgc cgttatttgt cggcatattt
     7681 ttaatggcat taccgcctgt gtatctgcaa gaccttccat taacgcttcg tcctctgctc
     7741 agtaacggct tattggtcgg gattttactg gctgttctta tggataacct tattccgtgg
     7801 gaacgtatcg aataatttgt tgaaaaagga ttgataatga agattgtcat tgcgccagac
     7861 tcttttaaag agagcttaag tgcagaaaaa tgttgtcagg caattaaagc cgggttttcg
     7921 accctctttc ccgatgtgaa ctatatctgt ttgccgatag cggatggcgg cgaaggaacg
     7981 gtggatgcga tggtcgccgc gacgggcggc aacatcgtga cgcttgaagt ctgcgggccg
     8041 atgggcgaaa aagtgaatgc tttttatggc cttaccggcg acgggaaaac ggcggtgatt
     8101 gagatggcgg cagcaagtgg cctgatgctg gtcgcgcctg aaaagcgtaa tccgttgctg
     8161 gcctccagtt ttggtacggg ggagttaatt cgtcatgcgc tggataacgg cattcgccat
     8221 attattctcg gcattggcgg cagtgcgacg gtcgacggtg gtatgggcat ggcgcaggcg
     8281 ctcggtgtgc gtttccttga tgccgacggt caggtgctgg tggctaacgg cggtaattta
     8341 gcgcgcgtgg caagcattga gatggatgaa tgcgatccgc gtctggcgaa ttgccatatt
     8401 gaagtagcat gtgacgttga taacccgctg gtaggggcac gcggcgcggc ggcggtgttt
     8461 ggcccgcaaa aaggggcaac gccggaaatg gtcgaagagc tggaacaggg gctgcaaaat
     8521 tacgcccgtg ttttacaaca gcaaactgaa atcaacgtct gccagatggc gggcggcggc
     8581 gctgcgggcg gtatgggtat tgcggcggcg gtatttctca atgcggatat taaaccaggt
     8641 attgagattg tgttgaatgc ggtcaatctt gcgcaggcag tgcagggcgc agcactggtg
     8701 atcaccgggg aagggcgcat cgactcgcaa acggcaggcg gtaaagcgcc gctgggtgtg
     8761 gcgtcggtgg cgaagcagtt taatgtaccg gtgattggga ttgctggcgt attgggtgat
     8821 ggcgtggaag tggtgcacca gtacggcatt gatgcggtat tcagcatttt gccacgtctg
     8881 gcacctttag ccgaagtact cgccagcggt gaaaccaatc tcttcaacag cgcacgaaat
     8941 attgcctgcg ccattaaaat aggtcaggga attaaaaact gacccttacc tttaaagcgg
     9001 atgcgattta tatcgcataa gagtgcagta ctcatgccgg atgcggcatg agtactatat
     9061 ccttcctgaa aatcgcgcaa attctatata ttgcagagat catgtaggcc tgataagcga
     9121 agcgcatcag gcaatgttac aaaaaaagcc acggtataaa ccgtggcaaa atccaacata
     9181 gctaaaaata atcaggcgag tggtatgact taaatctcta cgtcgcggtt gcaatctttc
     9241 gagtaaatat agctgaacgc ttcaccacgc cctacaccat aaccagcctg taaagaataa
     9301 gcgcccataa agatgtaatc gccttttttc accgggatcc agttattgtc gaggttataa
     9361 accccctgac cggaaagaat ataggcaccg tgttcctgaa catgtgtttc gatataaccg
     9421 tggctggcac ctggtgcaaa agagaggata tgcatgttca tatcaaaacc taactctttg
     9481 ggcagaaaat ccagcaggat cacatcatcc ataccttcat agtgaatgcg ttccagttcg
     9541 ctggcattgc cagaaaccag ccacggtgca tggccttcta ccggaacata gcggcgctta
     9601 tataaaaaga tttggctgtc ttcggcctgg gcgttaacaa acgtcattaa ggagcctggt
     9661 ggggcaataa agatagccac cttcgcttaa ggcaaatgtt ttgccttcgg ctttggcagt
     9721 gatatttcca gagatcacat acaggaacgt ttcaatgcct tcgccaccga agccctgttg
     9781 gttgccaccg ttttgatgca gtgtgaccag ataatcaaca aaagaggcac ccagctttgg
     9841 cgtggagagg attgtcgcgt cacaattttc aaagcccgga ataatatttt ttaccagacc
     9901 atccggggtt aacagtgcga aattaccgtg tttaacaatc gcacggttag ccagtaaatc
     9961 atcgcggtaa ccggtgacgt tatttaaata tcccatttat gactccttat ttctgccagg
    10021 caagttgata aagcatgagt gccaacgttt tgaccccttc ggcaaggtcg gtaatattgg
    10081 tgcgttccgc cgggttatgg ctgatcccgt tgatgctggg aatgaaaatc atgcaggttg
    10141 gtacgcgagg cgcgaaaatt tgcgcgtcgt gcccggcacc actgtgcatc acccggtaat
    10201 tcagtttttc actttcacac aattctgtca gggtggcgac cagctcctta ttcatcggca
    10261 cgggttcttc gtccatccat aaatcgatat caataccaat gtccatttca tcgcaaatcg
    10321 cccgcatgtc gttttctaac tgttgggtga aatcgcgcag cacggcagcg tcggtatgac
    10381 gacaatcaat ggtgaacgtg gttttacccg gcaccacatt taccgtattc gggcgcggct
    10441 ctactttgcc aaaggtcaga accagcggat cgcccatcct tttcgctttt tcgaccgact
    10501 gatggcaaat gcgactgaaa gcgtaaactg tatcacgacg ataacccatc ggcgtggtgc
    10561 ctgcatggtt tgattcgccg ttcagcgtta ccgtataacg acgctgcccg acaattgcat
    10621 tcaccacgcc aattgattgc ccattacttt ccagcacaca gccctgttca atatgcagtt
    10681 caacaaaggc tttaatatcc tgacgcggag ttagtggggc gttcggaaga gtaaatccgc
    10741 aagccttcat cgcatcgaca aaactatttc ctttggcatc acagatattc cgcacgtcgt
    10801 caggattcgc cagcccaaag atatttttac tgccccagaa gacatacggg aagcggctgc
    10861 cttcttcttc tgccatcgcc accacttcga ccgtacgtag cggcgcgccg tattgcgttt
    10921 tcagccagtc aattgccagc cacgccgcca gcgcgccgaa ttgcccgtcg aggttaccgc
    10981 cgttaaccac ggtatcgata tgcgaaccgc tcagaatcac ttcctgtgga tattcggtac
    11041 cattcaggcg accgtataaa ttccctactt catcgaaacg tgtttccagc ccacttgctg
    11101 ccattctttt tttaaattgc tgctgggttt ccagccattc cggcgaataa agtaaacggg
    11161 tcatcccacc cgttgggtca gcgccaaaag aggaaagcca gggcagcgtt tcttctatgg
    11221 cttgacgaaa atgtgtaatc ataagaaagt cctgtctcaa taattattgc gcaaagggat
    11281 ttttcgtttc gtatgacgtg ttataaagcg cgtcggaaat taaatactgg taaatatcat
    11341 caacaatttc gatgccttcg acggcggctt tgcgttgttt aatatcctga tcctgtccgg
    11401 gataataaac ttggttaaaa ccgggcgcgg gggtaatggc atttaattcg cgcatggtct
    11461 ggctaagatg ttgacggaat aattcgctgg aggagaaaaa gttcggatta ataactatat
    11521 gtaattgccc caaattacgc cctgcgtgta aatcgtcata catcgaacta acctgtcgcc
    11581 cgaacggtaa gccgagtaag acgcctgaga ggacgtcaat catcatcatc aggccatacc
    11641 cttttggccc ggcggcgggg agcagagcat gtaccgcgaa cggatcggtt gttggtgcac
    11701 cgtttttatc gaccgcccag gtatccggga tagacatatt acgcgagcgg gcgtcgagca
    11761 cttttcccca tgcctgtacg gtagtcgcca tatcaaaggt aaggatctcg tcgccttctc
    11821 ccggcgcggc aaaggccagg gggttagtac cgtagtaaat ttccgcgccg ccaaacggca
    11881 ccaccattgg atcggactgg cacatcgaaa tgccaatgaa tccggcgcgg gctgcctgct
    11941 gcacaaaata agagattgcg ccgctgtgac ccatccggct gataccgacc accgcaacgc
    12001 cattttgctg ggcggttttg atggcatgtt ccatacccat tttcgccgcg acctgtccgg
    12061 cggcattgtc ggcatgtaaa attgccgagc acggcccggt ttcctcaaga cgaaactccg
    12121 gttcgcggtt ggtgccgcct tttgaaatgc gttccgcgta gtattccacg cgcaccgcgc
    12181 catgagagtg gatccctctg gcatcggcgt aaaccaatac ttcagccacg gttgcagcgt
    12241 gctcacgttt taacccagcc tggcagagtt tattctcaat tagctggtgg agtgtttccc
    12301 gactgatttt catctgtctt cctttttaac gacggtgtga agcatgactg caattaacat
    12361 acagggaaaa tatctggatt atgtgatcca gacaggcaaa aaaatatagt tagaatttat
    12421 ttgataatcc gctcgctttt aacctggttt ttaaaacaac cacgcttatt aaaaaataac
    12481 acgtaatagc atggcggtta tttgaattct tttgttaata atccctgtgt gatattcatc
    12541 accttattta cccgttgtca tcgataccgt aatcgccaca ttaacactgc tcgtgcaatt
    12601 gccatgggtg caatttttaa ggagttgtta tgatccacgc ctttattaaa aaagggtgtt
    12661 ttcaggattc ggtcagttta atgattattt cacgaaaact cagcgaatca gaaaatgttg
    12721 atgatgtttc cgtaatgatg ggtacgcccg ccaataaagc gttattagat accacaggtt
    12781 tctggcatga cgattttaat aacgccacgc cgaacgatat ttgcgtggca attcgtagcg
    12841 aagcggcgga tgcggggatc gcgcaggcga ttatgcagca gcttgaagag gcgctaaaac
    12901 aactggcgca ggggtcaggc agcagccagg cgttgacgca ggtgcgtcgc tgggacagcg
    12961 cctgtcagaa attacccgat gccagtctgg cgctgatttc agtggctggc gagtatgcgg
    13021 cggagctggc gaatcaggcg ctggatcgca acctcaacgt gatgatgttc tccgataacg
    13081 tcacgctgga agatgaaatc caacttaaaa cccgcgcgcg agaaaaaggc ttgctggtga
    13141 tggggccgga ctgcggtacg tcgatgattg ccggcacgcc gctggctttt gctaacgtga
    13201 tgccggaagg caacattggc gtcattggcg cttccggtac cgggattcag gaactgtgtt
    13261 cgcagattgc gcaggcaggg gagggaatta ctcacgcgat tggtcttggc gggcgcgacc
    13321 tcagccgtga agtaggcggc atcagtgcgc tgacggcgct ggaaatgctc agtgcagacg
    13381 agaaaagcga agtgctggca tttgtttcaa agccacctgc cgaagctgtg cgtctgaaaa
    13441 ttgttaatgc catgaaagca accggcaaac cgacggtggc gctgttttta ggttataccc
    13501 cggcggtggc ccgcgacgag aatgtctggt ttgcctcctc gctggatgag gccgcacgcc
    13561 tggcttgcct gctttcacgc gtcacggcgc gacgtaacgc aatagcgcct gtcagcagcg
    13621 gatttatttg cggtttgtat accggcggta cgctggctgc cgaagcggcg ggattacttg
    13681 ctggacacct tggcgtggaa gccgacgata cccatcatca cggcatgatg ctcgacgccg
    13741 atggtcacca gattattgac ctcggcgatg atttctacac cgtcgggcgt ccccatccga
    13801 tgatcgaccc agccttacgc aaccagttaa ttgccgatct cggcgctaaa ccacaagtgc
    13861 gcgtattgct acttgatgtc gtgattggct tcggtgcgac tgccgatcct gccgcctcgc
    13921 tggtaagcgc ctggcaaaaa gcctgtgccg cgcgttcaga taatcaacca ctgtatgcca
    13981 ttgccacggt gacaggcact gaacgtgccc cgcaatgccg ctcgcagcaa atcgccacgc
    14041 tggaagatgc ggggatcgcg gtagtgagtt cgctgccgga agccaccttg ctggcggcag
    14101 cgttaattta tccgctctcg cctgccgcac agcaacatac accgtcatta ctggaaaacg
    14161 tcgccgtgat taacatcgga ttacgcagct ttgcgctgga gctacaaagc gccagcaaac
    14221 cggttgtgca ttaccaatgg tcgccagtcg ccggcggcaa taaaaaactg gctcgtttat
    14281 tagaacgttt gcaataaggg gttcccatgt ttacatcagt ggcgcaagcc aatgctgcgg
    14341 ttatcgaaca aattcgtcgc gctcgtccac actggctgga tgtgcaaccg gcttcttcac
    14401 ttatcagcga actaaacgag ggcaaaacac tgcttcacgc cgggccgcca atgcgctggc
    14461 aggagatgac cggacccatg aaaggggcgt gcgtgggcgc atgtctgttc gaaggctggg
    14521 cgaaagatga agcgcaggcg ctggcaatac tggagcaggg ggaagtgaac ttcattcctt
    14581 gtcaccatgt gaatgccgtc ggtccaatgg gcggtattac ttctgccagt atgccgatgc
    14641 tggtggtcga gaacgtgacc gacggcaacc gggcgtactg caacctcaac gaaggtatcg
    14701 gcaaagtgat gcgttttggc gcttacggcg aagatgtcct gactcgccat cgctggatgc
    14761 gcgatgtgtt aatgccagta ttaagcgcgg cgctggggcg catggagcgc ggtatcgatc
    14821 tcacggcgat gatggcgcag ggcattacga tgggcgatga gttccatcaa cgcaatattg
    14881 cttcctctgc actgttaatg cgtgcgctgg ccccacaaat tgctcgcctc gatcatgata
    14941 aacagcacat cgccgaagtg atggatttcc tcagcgtgac cgatcagttc ttcctcaacc
    15001 tcgcgatggc ttactgcaag gcggcgatgg atgctggcgc gatgatccgc gcaggcagca
    15061 tcgtcacggc aatgacccgc aacggcaata tgttcgggat tcgggtaagc gggctgggcg
    15121 aacgctggtt tactgcgcct gtaaacactc cgcaaggtct gtttttcacc ggcttctcgc
    15181 aggagcaggc gaacccggat atgggcgata gcgcgattac cgaaaccttt ggtatcggag
    15241 gtgcggcaat gatcgcagcg cctggcgtaa cgcgctttgt cggtgcgggt ggcatggaag
    15301 cggccagagc ggtatctgaa gagatggcgg aaatttacct tgagcgcaat atgcagttgc
    15361 agatcccagg ctgggatttt cagggcgcgt gcctaggact ggacattcgt cgcgtggtag
    15421 aaaccggcat tacgccactc atcaataccg gtatcgccca taaagaggcg gggatcgggc
    15481 agattggcgc aggcactgtg cgggcaccgc tggcgtgttt tgaacaggcg ctggaagcac
    15541 tggctgaaag catggggatt ggttgaggaa cgcgcaatga cgatcatcca tcctctgctt
    15601 gccagccgta gtgcaccgaa ttatcgccag tcctggcggt tagcgggagt gtggcgacgg
    15661 gcgattaacc tgatgacgga aagcggcgaa ctgttaacgc tgcatcgtca gggtagtggt
    15721 ttcggccccg gaggatgggt gcttcgccgc gcgcaattcg atgcgttatg cggtggatta
    15781 tgcggcaatg aacgaccaca ggttgtggct caagggattc gcctcgggcg tttcacggtt
    15841 aaacagccac agcgttattg tttgctgcgt attacgccgc cttcgcatcc tcaaccgctt
    15901 gcagctgcat ggatgcaacg cgcggaggaa accggacttt tcggaccact ggcgatggcg
    15961 gcaagcgatc cgctgcccgc tgaattacgc cagtttcgtc actgttttca ggccgcgctc
    16021 aatggcgtta agaccgactg gcggcactgg ctgggtaaag gccccggatt aacgccaagt
    16081 catgatgaca cgttgagcgg aatgctgctg gcggcctggt attatggcgc tttagatgcg
    16141 cgcgccggtc gcccgttttt tgcctgttcc gacaatcttc aactcgttac cacagcggtg
    16201 agcgtcagtt atttacgtta tgccgcgcaa ggatatttcg cctcgccact cctgcacttt
    16261 gttcatgctc tgagttaccc gaaacgtacc gctgttgcga ttgattcgct gctggcgctg
    16321 gggcatacgt caggggcaga tacgctgctg gggttctggc ttggtcaaca attattacaa
    16381 ggaaaaccat gaaaacactg gttgtagctc ttgggggcaa cgccttactc cagcgcggtg
    16441 aggcgctgac ggcagaaaat caatatcgca atatcgccag tgctgtaccc gcgctggcac
    16501 gtctggcccg ttcttatcgg ctggcgattg ttcacggcaa cgggccgcag gtggggctgc
    16561 tggcgttaca gaatctggcg tggaaagagg tagaaccgta tccgctggat gtgctggtgg
    16621 cggaaagcca ggggatgatt ggctatatgc tggcgcagag cttgagcgca cagccgcaga
    16681 tgccgcctgt tacgacggtg ctgacgcgca ttgaggtttc gcctgatgat ccggcgtttt
    16741 tgcagccaga gaaatttatt ggtccggttt atcagccaga agaacaagag gcactggaag
    16801 cagcttacgg ctggcagatg aaacgtgatg gtaaatactt gcgccgggtg gtggcgtctc
    16861 cgcaaccgcg taaaattctc gacagcgaag ccatcgagtt gttgctcaaa gaggggcatg
    16921 tggtgatttg cagtggcggc ggtgtgcctg tgacggaaga cggggcaggg agtgaagcag
    16981 tgattgataa agatctcgcc gctgcgttgc tcgccgagca gattaatgcc gatgggctgg
    17041 tgatcctcac tgatgctgat gcggtatatg aaaactgggg aacgccgcag caacgcgcca
    17101 ttcgccatgc cacaccggat gagttagcgc cattttccaa agtcgatggt tcgatggggc
    17161 cgaaggtaac ggcggtgagt ggttatgtca gaagccgtgg taaacccgcg tggattgggg
    17221 cgttatcgcg aattgaagag acgctggcgg gcgaagcggg gacctgtatt tcgctgtagt
    17281 gatgagagca tccggcaatc ggtgcaccat gcctgatgcg acgcttgacg cgtcttatca
    17341 ggcctacaat gggtaccgga gcggtaagcc ggataaggcg tttacgccgc atccggcaat
    17401 caaatgagca cctgcttaac tgaacttact ctgcgcccaa atcacgccgc tggcatactc
    17461 tggcggcagc agcgggatta aggcttccag cgtcgcagtc agacgcgatg tgtcgctgtc
    17521 ggtcaaattc agatgcccca ctttacgccc cggacggact tctttgtcgt accagtgcag
    17581 atgcaccagc ggcaatttca gccagtcata attcgcatcg ctaccaatca gattgatcat
    17641 caccgacgga ttattcacta ctggttgcgg taacggcaga tcggtaatcg cccgcagatg
    17701 cagctcaaac tggctgatgc tggcaccgtt ttgtgtccag tgaccgctgt tatgcacacg
    17761 cggagccagt tcgttgatca gcagaccttg cggggtgaca aaacactcca tcgccatcac
    17821 gcccacatag cccagctcct gcataatcgc cgacagcatc tcttcggctt gcgcctgctg
    17881 ctgtgcgttg gcctgcggaa aagcgacgct ggtgcgcaaa ataccgtcct gatgcaggtt
    17941 atgcgtcagc ggataaaaca cggtgctgcc atcaaagccg cgcgcgccaa ccagcgacac
    18001 ttcaccagag aagttaatgc cctgctcgac aatacattcg ccgtaacact ctgccggtaa
    18061 ctgttcggtt tcattggcgc gtaaacgcca ttgaccgcga ccgtcatagc caccagtgcg
    18121 acgcttaaca atcgccagtt cacctaaacg atcaaacacc gcaggccact cgctgcgatc
    18181 ggcaagtaac tgccacggcg cggtcggcag gtggagctta tcgaaaagct gcttctgagt
    18241 cagacggtca gcaataatcg ggaacacatc gcggttcaca aaggccggat gacgcgccag
    18301 ctcgcgggtt aatgcggttt ccggccagcg ttcgatctca gcggtaatca cgctttgttg
    18361 aaaaggcacc gccgccggtt cagcgtccag cccgaccggc cagacagcaa tgcctaacgg
    18421 ttcgcctgcc tgacgcagca tacggcctaa ctgcccgtta ccgaggacgc aaacctgttt
    18481 catgccgcac ctcgcgggtc cgggttttcc agcacttcgt cggtctgggc tttgcgccag
    18541 tcattcagac gctggtgtag ctctttatcg tgagtcgcga gaatttgtgc tgccagtaat
    18601 gccgcgtttg ccgcgccagc tttaccaatc gccagcgtac ccaccggaat gccgcgcggc
    18661 atttgtacga tggagtagag gctatcgaca ccgctcagtg cggcgctctg tactggcacg
    18721 cccagcaccg gcaccagcgt tttggcggca atcatgcctg gcaggtgcgc tgcgccacct
    18781 gcgcccgcaa taatcacctg ataaccgttc tcttcggcgc tttcggcgaa gctgaacagt
    18841 ttatcggggg tgcggtgagc agaaaccact tcaacgtggt gcgggacatt caggatttcg
    18901 aagatttcgg cggcgaactg catggtagcc cagtcgcttt tggaccccat cacgatggcg
    18961 acacgcgccg gattattgcg ggaagacatg cgtcttaaaa ctcctgtggt gcacaactct
    19021 cggctttaga gggcacagag aatagcacgg aaagagagca aggaaaacgg ttgcgtggct
    19081 gtgaaatcag caaagttgcg ggttttttaa aacggaaaat gaatcagctc aacgtcatcc
    19141 gccgtgactt tcaccatcga accttccgta tgccaggcac cgagtaccac gcgaaaagca
    19201 ggttgctggt tggcgataag ttcatgtacc gccgggcgat gggtatgtcc gtggatcagc
    19261 cattgcacct gatgtttttc catcgcactg actaccgcgt tttggttaac gtccatgatc
    19321 gccagcgatt tactgctgtt ggcttcttta ctgttcgctc gcattcgcgc ggcaatgcgt
    19381 ttgcgcacaa acaacggcag ggcgaggaat agcatttgca gccagggttt gtggactttg
    19441 gcgcgaaaag cctgataacc cgcgtcatcg gtgcacagcg tgtcgccatg cataatcaac
    19501 acccggcgac cataaagttc gagcaccttt tcttccggca ataatgtcat gccgctttca
    19561 cgggcaaagc gtttgccgag cagaaaatca cggttgccat gaatgaaata acagggaacg
    19621 cctgaatcgg acaccgcttt gatcgctgcc gccatctggc gatggagtgg gttgggatcg
    19681 tcgtcgccaa tccatgcttc aaacagatcg ccaagaatat acagcgcgtc ggccttgcgg
    19741 gcttcccccg ctaaaaaacg cagaaaaccg gcggtgatcg ccggttcttc cacgcagaga
    19801 tgaagatctg caataaagag tgtcgccacg attactcgct aacggtcacg ctttcaatga
    19861 taacgtcttc ttttggcacg tcctggtgca taccgctacg accggttgca acacctttga
    19921 ttttgtctac cacgtccatg ccttcaacca cttcagcaaa cacgcagtag ccccaacctt
    19981 gcaggctttc gccagagaag ttcaggaagt cgttatcaac cacgttgatg aagaactgtg
    20041 cagttgcaga gtgtggagcc tgagtacggg ccattgccag cgtaccacgg gtatttttca
    20101 gaccgttgtt ggcttcgttt ttgatcggtt ctttggtggc tttttgtttc atgcccggtt
    20161 caaaaccgcc gccctgaatc ataaagccgt tgataacacg gtggaaaatg gtgttgttgt
    20221 aaaaaccttc gcggcagtag tccaggaagt ttttaactgt ttcaggtgct ttatcgtcaa
    20281 aagttttgat gacaatatcg ccgtgattgg tgtggaaagt aaccattttt gcatcctgtt
    20341 ccgtttgatt ggtgcttcaa cccagttcgg gtcatatata gggtggtgtt atagcataac
    20401 cgcacgatcg gatcatcacg caatgtatgc tgattcgcgc gggaaatatg ggtattatac
    20461 gcaactcaat tacccacaca tgtctaaacg gaatcttcga tgctaaaaat cttcaatact
    20521 ctgacacgcc aaaaagagga atttaagcct attcacgccg gggaagtcgg catgtatgtg
    20581 tgtggaatca ccgtttacga tctctgtcat atcggtcacg ggcgtacctt tgttgctttt
    20641 gacgtggttg cgcgctatct gcgtttcctc ggctataaac tgaagtatgt gcgcaacatt
    20701 accgatatcg acgacaaaat catcaaacgc gccaatgaaa atggcgaaag ctttgtggcg
    20761 ctggtggatc gcatgatcgc cgaaatgcac aaagattttg atgctttgaa cattctgcgc
    20821 ccggatatgg agccgcgcgc gacgcaccat atcgcagaaa ttattgaact cactgaacaa
    20881 ctgatcgcca aaggtcacgc ttatgtggcg gacaacggcg acgtgatgtt cgacgtcccg
    20941 accgatccga catatggcgt gctgtcgcgt caggatctcg accagctgca ggcaggtgcg
    21001 cgcgttgacg tggtcgacga caaacgcaac ccgatggact tcgttctgtg gaagatgtcg
    21061 aaagagggcg aaccgagctg gccgtctccg tggggcgcgg gccgtccagg ctggcacatt
    21121 gaatgttcgg caatgaactg caagcagctg ggtaaccact ttgatatcca cggcggcggt
    21181 tcagacctga tgttcccgca ccacgagaac gaaatcgcgc agtccacctg tgcccatgat
    21241 ggccagtatg tgaactactg gatgcactcg gggatggtga tggttgaccg cgagaagatg
    21301 tccaaatcgc tgggtaactt ctttaccgtg cgcgatgtgc tgaaatacta cgacgcggaa
    21361 accgtgcgtt acttcctgat gtcgggccac tatcgcagcc agttgaacta cagcgaagag
    21421 aacctgaagc aggcgcgtgc ggcgctggag cgtctctaca ctgcgctgcg cggcacagat
    21481 aaaaccgttg cgcctgccgg tggcgaagcg tttgaagcgc gctttattga agcgatggac
    21541 gacgatttca acaccccgga agcctattcc gtactgtttg atatggcgcg tgaagtaaac
    21601 cgtctgaaag cagaagatat ggcagcggcg aatgcaatgg catctcacct gcgtaaactt
    21661 tccgctgtat tgggcctgct ggagcaagaa ccggaagcgt tcctgcaaag cggcgcgcag
    21721 gcagacgaca gcgaagtggc tgagattgaa gcgttaattc aacagcgtct ggatgcccgt
    21781 aaagcgaaag actgggcggc ggcagatgcg gcgcgtgacc gtcttaacga gatggggatc
    21841 gtgctggaag atggcccgca agggaccacc tggcgtcgta agtaattgcg ctgttgccgg
    21901 atgcgagttt tcgcatccgg ttatcgcctg cgccaccaca acattcccat aagcaccatc
    21961 cccggcaacc acacccacat caattcagaa attatcacct gatgcccgta cggcgtggtg
    22021 taacgagaca atgcaaacgg cgcgacttta atcacctgcc agggagcgaa aaagcgttca
    22081 tctgaccacg gccacagcca gccaacgccc ttaccgccag tggttaccga atccagcaag
    22141 ctgtgcgata gcaacgagac ggttaaaaac agccagcagc gaatcagccc agccctgaac
    22201 catcggcgtc caatcagcac acataacagc gggacaacaa acgcaaacac cagcgaatgg
    22261 gtaaacccgc gatgaccaaa aacattgccg taagcaacgc caaatttaaa cgacaatacg
    22321 tcggcgtcgg gcagcatcgc caggatgatt ccggcaaata acagacgcgg agggatgact
    22381 ttcgaaccca accctaaacc aatgcatagg ggaacggcgg cgtgcgtaat aacggttggc
    22441 atgatggtcg cttcggcaaa atgtcgatgc tatcagcatg gatgaacggg gcgtagaggg
    22501 caaaagtctg aaaagagaac cggcctgttg atacaggccg ggaaagggat caggcaacaa
    22561 cctgtacgct gtgacctgca aaactcactg tctgaccggc gacgattttg cagcgtttgc
    22621 gcgtttcaac cgcaccgtcg actttcacct ggccttcggc aatcgcgatt ttcgcctgcg
    22681 cgccgctttc gctccagcct tccagtttca gcaagtcgca cagctcaacg tgcggatgtt
    22741 tacctaaaga aaatgtcgcc atgttactca tcctgtggat catgatattc aacgcacgcc
    22801 tgtagcgtgt tttcaatcag cgtggcaacc gtcatcgggc caacgccgcc gggaacaggc
    22861 gtaatgtatg aggcgcgttt agccgcgtct tcaaacacga cgtcgcccac aactttgcca
    22921 ttttccagac ggttgatgcc gacatcaatc acaattgcgc cttctttgat ccagtcaccg
    22981 ggaataaagc ctggcttgcc aacggcaacg atcaacagat cggcattttc tacgtgatga
    23041 cgcagatttt tagtgaagcg gtgagtcacg gtagtggtgc aacctgcgag caacagttcc
    23101 atgctcattg ggcgaccgac gatattcgat gcgccaatca ccacggcgtt gaggccgaag
    23161 gtatcaatgt tgtaacgctc aagcagcgtg acgataccgc gcggggtgca gggacgcaga
    23221 cgcggcgcgc gctggcacag acgaccgacg ttgtaaggat ggaaaccgtc cacgtctttg
    23281 tccggatgaa tacgttccag cactttgacg ttatcaatac ccgccggtaa cggcagttga
    23341 accagaatgc catcgatggt gttgtcggca ttcagcgtat cgataagctc cagcagctcc
    23401 gcttcgctgg tggtttccgg gaggtcataa gagcgggaga cgaacccgac ttcttcacaa
    23461 gccttgcgtt tgcttgcgac ataaatttgc gatgcagggt tactacccac cagcacaacg
    23521 gccagtcctg gtgcccgcag tccggctgca atacgcgcct gaactttttg agcaacttca
    23581 gagcgcacct gctgcgcaat cgttttaccg tcaataatct ttgctgccat cagagagagg
    23641 attccatctg ttacgtaggt cgaaggggat gcgtctattt tgtcagaagc ggggcgcgct
    23701 gtcaggtttc gtttcagatt tatcgcgtga agcgacctct tgcgaaggtt aggcgcaccg
    23761 tcgctgaggt cgctgagatt gaaagcttca tttttcgtcc atgatggcgt tgtaaatctg
    23821 gaactgattt atttccttgt ttaaggatta agataattta agaaatacct
//
LOCUS       DAZKIV010000064        23845 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZKIV010000064 DAZKIV010000000
VERSION     DAZKIV010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.064"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(552..719)
                     /locus_tag="ACHOT6_002454"
     CDS             complement(552..719)
                     /locus_tag="ACHOT6_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9947655.1"
                     /translation="MDTITDKKAEQLESQGLWRRAAARWLDVMKEAHTDPQREHIARR
                     REICLANFRML"
     gene            complement(721..1023)
                     /locus_tag="ACHOT6_002455"
     CDS             complement(721..1023)
                     /locus_tag="ACHOT6_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9947656.1"
                     /translation="MIRDDKAEALENSGLWRRAAERWGELFRQAKTDEERHYITQRRA
                     QCTAKATRPKTTEWGHVCAVRAAATRTLKDMGIDQKKEDPLRGTKYRGETGRRDGS"
     gene            1108..1494
                     /locus_tag="ACHOT6_002456"
     CDS             1108..1494
                     /locus_tag="ACHOT6_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947657.1"
                     /translation="MRLTQYKKEILSYFTPDNLAWVTAEIGSPPFDVSGVAYLLHGMD
                     SFNSRYQIESTRRTLEAMVRDGLLERINVYERREGAGDGVRRKVARYGLPGHSVVVRD
                     GEGRNGAIDGECIRINATDVVLVEGQ"
     gene            complement(1941..2234)
                     /locus_tag="ACHOT6_002457"
     CDS             complement(1941..2234)
                     /locus_tag="ACHOT6_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9947658.1"
                     /translation="MMTDSVAQKLEERGLWRRAATRWAEVLLHAETDSEREEAARRRA
                     ICISKTRRMPEQFVTFGDVKKAADRTLKEMGINPQDEWRNYSFSDAGDDLALP"
     gene            complement(2254..2670)
                     /locus_tag="ACHOT6_002458"
     CDS             complement(2254..2670)
                     /locus_tag="ACHOT6_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFP9947659.1"
                     /translation="MNQQTTQQDQTTRKPSRRIPWQHMTKRQRKNRRRINRLAEYWPE
                     LFGAENIKPLKAGVIHDMLQDAKARELAIGHGVLRGALTSYIHTTRYLKAIVAGGARY
                     GLNGQPCGEVTEEDKSVASELLKRRLEQMKQKNSQQ"
     gene            complement(2687..3136)
                     /locus_tag="ACHOT6_002459"
     CDS             complement(2687..3136)
                     /locus_tag="ACHOT6_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9947660.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADALARHEKGDLISVAGNMQISQWTGQNGEIRQGWQVIADSVISA
                     RSARPGGKRGQQGKATEALERAKRQTGTRGNQPPAGGDDPFSDDIPF"
     gene            complement(3133..3582)
                     /locus_tag="ACHOT6_002460"
     CDS             complement(3133..3582)
                     /locus_tag="ACHOT6_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947661.1"
                     /translation="MTEKHAKKSHSHAIDLTEPESVVRFVSVRGRATLYIPDEHLHHC
                     DKHKLPILIVWKRTVYADVTWLNDSLMLIHRDLFEREEFRRDIEERAGKIYEKYAANS
                     KRAARAITYHFMTLYDLKAEDAEKAACDLFDMTMGIIQEYRNKERRP"
     gene            complement(3870..5696)
                     /locus_tag="ACHOT6_002461"
     CDS             complement(3870..5696)
                     /locus_tag="ACHOT6_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9947662.1"
                     /translation="MKSAPNLKKQPRGKKHADTEVIIFAGSDAWAHAKQWQEQDGRLA
                     GDNVPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAVAGVRDA
                     DYYPEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGA
                     SQRGEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQN
                     AIKSAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPP
                     AEGETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSG
                     KSVMAEICTMLAGKANTVSASMKALEDARERALVVGFSLIILPDMTRYAGDGAGIKAI
                     TGGDKVAIDPKHKAPYSTRIPAVVLAVNNSAMSFSDRSGGISRRRVIFNFSEVVPENE
                     RDPMLPEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCG
                     YLMSSVMCDGLLVGNAEIIPFSPRRYLYHAYLSYMRAHGFSKPVTLTRFGADMPGAMA
                     EYGREYMKKRTKEGVRSNVILTDESEDWLPSCAPVKNEGDKN"
     gene            complement(5873..6064)
                     /locus_tag="ACHOT6_002462"
     CDS             complement(5873..6064)
                     /locus_tag="ACHOT6_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947663.1"
                     /translation="MQQITLAEASLQAQQVEVISRMIERNHEEIDDHDLSVIAGLIKQ
                     LSSNVAVWLMTEEEKRGEQ"
     gene            complement(6109..6318)
                     /locus_tag="ACHOT6_002463"
     CDS             complement(6109..6318)
                     /locus_tag="ACHOT6_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947664.1"
                     /translation="MCDFSDMTVDEAAKSAMQAELICSLMMGNTGGMTEGEIESLLAL
                     IKQLTGKAGSWLIAASGDDAICVND"
     gene            complement(6322..6507)
                     /locus_tag="ACHOT6_002464"
     CDS             complement(6322..6507)
                     /locus_tag="ACHOT6_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947665.1"
                     /translation="MLKTFRVFSRAVNKRGHTIGITQNVKAENVQAAISTVINKSAKA
                     GLSGVIIHAVTCIQDEE"
     gene            complement(6500..6670)
                     /locus_tag="ACHOT6_002465"
     CDS             complement(6500..6670)
                     /locus_tag="ACHOT6_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9947666.1"
                     /translation="MAVSARPYFVWRFMQCLTDSIAIFTVTAATEREARARLPHAHLI
                     FVARIRQEVTHA"
     gene            complement(6725..6808)
                     /locus_tag="ACHOT6_002466"
                     /pseudo
     CDS             complement(6725..6808)
                     /locus_tag="ACHOT6_002466"
                     /inference="COORDINATES: protein motif:HMM:NF022029.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7142..7330
                     /locus_tag="ACHOT6_002467"
     CDS             7142..7330
                     /locus_tag="ACHOT6_002467"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947667.1"
                     /translation="MTNEELQEIALSILERQREKAQRQRRLLLNPLPLTRPTRFALED
                     FVEKYPRRLKATKNRPPG"
     gene            complement(7637..8485)
                     /locus_tag="ACHOT6_002468"
     CDS             complement(7637..8485)
                     /locus_tag="ACHOT6_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFP9947668.1"
                     /translation="MKRPNMPVQGQGFVQSEELSQQYFAAIIPVSPGIIGGYETGIVS
                     ARALHKALGVGRDFTTWIKGRIEEYGFKKGVDFEVVENLYFDSPKRGSQSVNIEQFDS
                     GWTTKRGGDRRSEDYLISTNMAKELAMVERTEQGRAIRQYFIKCEEELHKAAPERAAA
                     LRRELKARVTVASFFKPMCAALELYRAELGKETHQHHYTTESNMLARIVLGGMTAKQW
                     AQANGIKGKPRDSMSVMQLEHLSYLDQSNITLIELGMTYQQRKAELIRLSQRWLARRM
                     GEHEHA"
     gene            complement(8553..8768)
                     /locus_tag="ACHOT6_002469"
     CDS             complement(8553..8768)
                     /locus_tag="ACHOT6_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9947669.1"
                     /translation="MTANHQEAIPTTGYIRRCNLAKLLDTSVSTIDRWVRIGKLPKPT
                     KLGEKNTVFNAVEINHWLNERRNNTKQ"
     gene            complement(9155..9805)
                     /locus_tag="ACHOT6_002470"
     CDS             complement(9155..9805)
                     /locus_tag="ACHOT6_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947670.1"
                     /translation="MINILKKSPIERLCASVSITPHEMALALADLNPSMRIGDVPQDK
                     FEQVESARTHIARAIRLHSGVKTSKDEPYRAGDIFLASFPFMEAGTPEAIIKAVTDTI
                     DDLRGTKNWEEKALNLGGRDLVRYIKETSRSGRGQHRKLDEEQGNMKMMGLLVLLLVK
                     KSGTTAYIQDGEPNRSAIYRDMEALMKEKGISPKGLAKSTFMQKISAALLAVSQAD"
     gene            complement(9802..11064)
                     /locus_tag="ACHOT6_002471"
     CDS             complement(9802..11064)
                     /locus_tag="ACHOT6_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9947671.1"
                     /translation="MPKLTDIQIRAWIKSGERFEGRSDGGGLYLRYRKEDQIPTWRFR
                     YKFAGKARAMVIGSYADYSLSKARETAKELSARVALGYDVAAEKQERKAEALAKIEAE
                     KKAVRVSELAAEYFERQILPRWKHPDILRRRIDKDINPRIGHMKAEDVKPRHIDEMLK
                     AIVDRGAPTIATDVLRWTRRIFDYGVKRHVLEINPCSAFEVSDAGGKESSRDRWLTRE
                     ELIRFFQAMRTAKGFSRENELTFKLLLALCVRKMELCAARWEEFDLDNAVWHLPEERS
                     KNGDPIDIPLVHPVIEWLRELRVLSCNSAWVLPARKMQHRMIPHIQESTLPVALAKVR
                     AEMPEVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRYQYFNERRD
                     ALAKWTTLLVSLERGEHYNVTPIRRAKS"
     gene            complement(11254..12117)
                     /gene="yicC"
                     /locus_tag="ACHOT6_002472"
     CDS             complement(11254..12117)
                     /gene="yicC"
                     /locus_tag="ACHOT6_002472"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFP9947672.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNVE"
     gene            12244..12960
                     /gene="rph"
                     /locus_tag="ACHOT6_002473"
     CDS             12244..12960
                     /gene="rph"
                     /locus_tag="ACHOT6_002473"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFP9947673.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13026..13667
                     /gene="pyrE"
                     /locus_tag="ACHOT6_002474"
     CDS             13026..13667
                     /gene="pyrE"
                     /locus_tag="ACHOT6_002474"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFP9947674.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(13704..14300)
                     /gene="slmA"
                     /locus_tag="ACHOT6_002475"
     CDS             complement(13704..14300)
                     /gene="slmA"
                     /locus_tag="ACHOT6_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFP9947675.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(14407..14865)
                     /gene="dut"
                     /locus_tag="ACHOT6_002476"
     CDS             complement(14407..14865)
                     /gene="dut"
                     /locus_tag="ACHOT6_002476"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFP9947676.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(14843..16063)
                     /gene="coaBC"
                     /locus_tag="ACHOT6_002477"
     CDS             complement(14843..16063)
                     /gene="coaBC"
                     /locus_tag="ACHOT6_002477"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFP9947677.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16235..16903
                     /gene="radC"
                     /locus_tag="ACHOT6_002478"
     CDS             16235..16903
                     /gene="radC"
                     /locus_tag="ACHOT6_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9947678.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17120..17356
                     /gene="rpmB"
                     /locus_tag="ACHOT6_002479"
     CDS             17120..17356
                     /gene="rpmB"
                     /locus_tag="ACHOT6_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFP9947679.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHCFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            17377..17544
                     /gene="rpmG"
                     /locus_tag="ACHOT6_002480"
     CDS             17377..17544
                     /gene="rpmG"
                     /locus_tag="ACHOT6_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFP9947680.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            17642..18451
                     /gene="mutM"
                     /locus_tag="ACHOT6_002481"
     CDS             17642..18451
                     /gene="mutM"
                     /locus_tag="ACHOT6_002481"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFP9947681.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(18490..18969)
                     /gene="coaD"
                     /locus_tag="ACHOT6_002482"
     CDS             complement(18490..18969)
                     /gene="coaD"
                     /locus_tag="ACHOT6_002482"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFP9947682.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(18977..20254)
                     /gene="waaA"
                     /locus_tag="ACHOT6_002483"
     CDS             complement(18977..20254)
                     /gene="waaA"
                     /locus_tag="ACHOT6_002483"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFP9947683.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            20667..21725
                     /gene="rfaQ"
                     /locus_tag="ACHOT6_002484"
     CDS             20667..21725
                     /gene="rfaQ"
                     /locus_tag="ACHOT6_002484"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFP9947684.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            21722..22846
                     /gene="waaG"
                     /locus_tag="ACHOT6_002485"
     CDS             21722..22846
                     /gene="waaG"
                     /locus_tag="ACHOT6_002485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9947685.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            22839..23636
                     /gene="rfaP"
                     /locus_tag="ACHOT6_002486"
     CDS             22839..23636
                     /gene="rfaP"
                     /locus_tag="ACHOT6_002486"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFP9947686.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5526 a         6022 c         5941 g         6356 t
ORIGIN      
        1 ttgatactga caattcaacg tccgtcatcg ctcccccaat gtgttgaggt taggtagaaa
       61 tgataacaaa tacaccgctc ctgcccctgt aacctcccct tttttatacg ctcatttttg
      121 cgcacttttt cgatcatcac gtatcggttt cgttttgcgc ttcctgttgc gtttcgtttt
      181 tgagaaaaat gcgcagtttt ttgcgcactt tttgaggtgt ctatccccct gcaagccgcg
      241 tcatatctgg ctttgctctg tttgtctgtg tgatcgattt tgtgatcagt tttatgatcg
      301 atttgcgcac ttttttgcgc attttttcag ggatttttca ccccatcgaa gaggcttcta
      361 aggggctgtg aagtgggtag cattttgctg tgatggaagg actaccgcta ctgtctgata
      421 atggtgacgg aggccgctat gttcggctct gattgcagcc tgccgtatat tcaccaattt
      481 acctggctgt tatttccata acccgtaatg tcgccgatcc cgtctgattc ccatatcgtc
      541 aacagtctta tttacagcat cctgaagttc gcaaggcata tttcccgccg tctggctata
      601 tgttctcgct gcggatcggt gtgggcctct ttcattacgt caagccaacg agcggcggct
      661 cttcgccata gtccctgaga ttccagttgt tcggcttttt tatcagttat tgtgtccata
      721 ttaacttccg tctcttcgcc ctgtttcacc cctgtacttc gtgccccgta aaggatcttc
      781 ttttttctgg tcaatcccca tatctttaag cgttctggtc gctgctgccc tgacggcgca
      841 tacatgcccc cactctgttg tttttggtct ggtggctttt gccgtgcatt gcgcccttcg
      901 ttgggtaata tagtgcctct cttcatcagt ttttgcctgt ctgaacaatt ctccccagcg
      961 ttcagcagca cgccgccaga ggccgctatt ctccagcgct tcagccttgt catcacgtat
     1021 catctgtacc ccgccgattc tgtcctttca aaatttgtct tatggcttat tgtatgaggg
     1081 attcatccta caacaagaaa ggtcactatg agactgacgc aatacaaaaa agagatcctc
     1141 agttacttca ctcccgacaa tctggcgtgg gttactgccg aaattggcag tccgccgttt
     1201 gatgtctccg gtgtggctta cctgctgcat ggtatggatt cattcaacag ccggtatcag
     1261 atcgaatcaa caaggcgcac gctggaagct atggtgcgtg atggtcttct tgagcggatt
     1321 aatgtgtatg agcgtcgtga gggcgctggt gatggtgttc gtcgaaaggt ggcgcgttat
     1381 ggcctgcctg gtcattctgt tgtggtgcgt gacggtgagg gccggaacgg tgcaattgac
     1441 ggcgaatgta tacgcatcaa tgctacagat gtggtgctgg tggaggggca ataaaaaacc
     1501 ctgaaagatt tctccgggtg ctccagttgc acgtggcaga aatccttcag ggtcagccgt
     1561 aacgggcctc tgtacagagg gctccagttg cccagagatg agcacccgct acagcctgtt
     1621 gtgataagcc gctttacagt gagctccagt tgctcaaaga ggcgcgtttt tatcacaacc
     1681 tgaccgcgat aacccgctgt acagagggct tcaattgccc aaagaggcgc attcttatcg
     1741 cggcatgacg aaatcaccgt gatgttttcg tcgttcactc atcccgatgt gtgggagatg
     1801 caccggaatg agtgccgaaa agccgctggc tggcttcatg cagtcggact cagttgcccg
     1861 gggcgaacac catcaacggc cttttggtgg tgattatagc gaatcgcgat aattgagcaa
     1921 atacggtgat gagatgcgta tcacggtagc gctaaatcgt cgcctgcatc actgaagctg
     1981 taatttctcc attcatcctg cggattaatc cccatctctt tcagcgttct gtctgcggcc
     2041 tttttcacat cgccaaaggt gacgaactgc tcaggcatac gacgggtttt actgatgcat
     2101 atcgcccgac gtctggcagc ttcctcgcgt tcactgtccg tttctgcgtg aagaagaacc
     2161 tcagcccatc gggtcgccgc acgtcgccat aaaccgcgct cctcaagttt ctgtgccacg
     2221 ctgtccgtca tcactggctt tacctccggc tgattactgc tgactgtttt tctgcttcat
     2281 ctgctccagt ctccgcttca gtagttcgct ggctactgat ttatcttctt ccgtgacttc
     2341 accgcaaggt tgcccgttca gtccataccg tgcgccgcca gcgacaatgg ctttcaggta
     2401 tcgcgtcgtg tggatgtatg aggttaatgc gccacgtaat acgccatgac caatcgccag
     2461 ttcccgcgcc ttcgcgtcct gcaacatatc gtgtatcacg ccagccttaa ggggttttat
     2521 attctctgcc ccaaacagtt caggccagta ctcagccagt cggtttatcc tcctgcggtt
     2581 ttttcgctgc cgctttgtca tgtgctgcca cggtatgcgc cgtgatggtt tacgtgtggt
     2641 ctggtcctgt tgtgtggtct gctggttcat gttgtgctcc tgtcattcag aacggaatat
     2701 cgtcagagaa cgggtcatca cctcccgctg gtggctgatt cccccgtgtt ccggtctgtc
     2761 gttttgctct ttccagtgct tcggtcgcct tcccctgttg cccccgcttc ccgccaggac
     2821 gcgccgaacg ggcactgatt acgctgtcag cgataacctg ccagccctgc cgtatttctc
     2881 cgttctgtcc ggtccactga ctgatctgca tgttacccgc cacgctgata aggtcgcctt
     2941 tttcatgtct cgccagcgcg tcggcctgct taccaaatgc caggactgat agccataacg
     3001 tcgcctgtcc gtcctgtgcc tgactgcatg gcaaagatac cgccatacgc gccagcgtca
     3061 tcggtgtgcc cttgctggtc tgttttacct gcgggtcgtc caccagccgt ccgtaagctg
     3121 caatctgtgc tgtcatggtc tgcgctcctt gttccggtac tcttggataa tacccattgt
     3181 catatcgaat aaatcacatg ccgctttttc tgcatcttcc gctttcaggt catacagcgt
     3241 catgaaatgg taagtgatcg cccgtgctgc tcttttgctg tttgctgcat atttctcata
     3301 gatttttcct gcccgctctt caatatcccg tcggaactct tcacgttcaa acaggtcacg
     3361 gtgtatcaac atcagcgaat cattcagcca tgtcacatcg gcgtataccg ttcgcttcca
     3421 gacaatcagg atcggaagtt tatgtttgtc gcagtgatgc agatgttcgt caggtatata
     3481 aagcgtggct ctgcctcgta ccgatacgaa cctcacaaca gattccggtt cggttaaatc
     3541 gatagcgtgt gaatgtgatt ttttggcgtg tttctctgtc ataaaatttt tctcaaagga
     3601 ataaataaaa atttatgccc aaaaggtgtt caactgttca ccttttgcta tttcgccttt
     3661 agattcatga agttaagggg tgaacgcttt ttgtaaaggt gttcaccaac tgttcaccct
     3721 gctcacccgc gactgttcaa aaatcataca aaaaggtgaa caggtgaata ctttggtgaa
     3781 tagttttaca aaaagtgttc acccattaac atattgatac taaatacatt tattgcaggg
     3841 tgaacagtgg tgaacagttt ttctataact taatttttat caccctcatt tttcactggt
     3901 gcacatgacg gcagccagtc ctcagactca tccgtcagta tcacgtttga tcgcacacct
     3961 tctttagttc tctttttcat atactccctg ccatattccg ccattgcgcc tggcatatct
     4021 gcaccgaagc gcgtcagtgt tacgggctta ctgaaaccgt gcgccctcat atacgagaga
     4081 taggcgtgat aaagataacg gcgtggactg aaaggaataa tctcagcatt accgactaac
     4141 aggccgtcac acattaccga cgacatgaga tagccgcaga agtccaccag cgaatccccc
     4201 tcacgcttta tcgccagtgc ttcttctgat ttctgctgct catataaaag gcgtctggct
     4261 tcgtcctgat cagcaaaccg tgtaagcaga tgacgaatca ctacagcaag ctcaccttct
     4321 attttttccg gcagcatcgg atcgcgttcg ttttccggta caacctccga aaaattgaat
     4381 atcacacgac gacgtgagat ccccccgctg cggtcactga atgacatggc gctgttgtta
     4441 accgccagca caaccgccgg aatacgcgtt gagtatggcg ctttgtgttt cgggtcaatt
     4501 gccaccttgt caccgcccgt aatggccttt atccctgcac catcacccgc gtagcgggtc
     4561 atatccggca gaataatcag tgaaaagcca accactaatg cgcgttccct tgcatcctcc
     4621 agtgccttca tgcttgccga taccgtgttg gccttacctg ccagcatggt acaaatctcc
     4681 gccataacac ttttaccgct tccaccagga cctgttacct caatgaataa ctgccagtcg
     4741 taccggttcg ccagcaccat aaataacgct gccagaacgc gatctgcctt gcggtcattc
     4801 tcagccaccg aacggcgcaa ccatttccag aaattcggcg catgtgttgc cagcgtttcc
     4861 ccctctgctg gtgggctgaa aggtaattca ctggcaatta acaaccagtc gtttttgtta
     4921 tgctcccgaa aattacctgt tctggtatca aataccccgt tactgaatcc aatcaggtta
     4981 cgggctgtat tccccattac aggcaaactt aacttcatgg tatcgaccgc cgatttaatg
     5041 gcgttctgcg aatagctgat ctccgcatca atgaaaatct gtgccatagc tcgctgtaat
     5101 tctttatcct gtactggctc ccatacaacg ccgttgtaat ggtgaacagt gtcagagtca
     5161 gcatgaatcg ccagttcacc gccataatgt gccaggagaa cttcgccgcg ttgacttgct
     5221 cccatctggt taagcgctag tgatgaagcg ttatcgtctt ttacccgctc ttttttcttt
     5281 acaggcagtt caactacctt tttcttttcc gcctgctctg cccgttcacg ttccagatat
     5341 tcgcgccagt tctcccgttt ctggctgtgc attccttcag ggtaataatc agcatccctg
     5401 acacctgcca ctgccagttt ctgcccgatg gtattaacaa gccccggacg caataacccc
     5461 gcctgataga gacgcacgcg atagcgtccg tccggtacga tttgcaggtt gtccagttcg
     5521 gcaagttgtt gctctccaag ccagacaggt ggcacgttat cgccagccag tcgcccgtcc
     5581 tgttcctgcc actgcttcgc atgtgcccac gcatcactgc cagcgaaaat aatcacttcc
     5641 gtatcggcgt gttttttccc tcgaggctgt ttttttaagt tcggtgcgct tttcatttct
     5701 tacctctgct gatcatcgtc atgtggctac cgtattcatt gcggtaaata tccccgcaca
     5761 taaattcggg gtgagttttg ctactggcag ttaagccagt attttttgat gccattgaat
     5821 taatctcctg aaggagtcgt cttaatcctt atttcccgtt tatttttagc cgttattgtt
     5881 cgccgcgttt ttcttcctcc gtcattaacc acaccgccac attgctggaa agttgtttga
     5941 taagtcctgc aataacagaa aggtcatggt cgtctatttc ttcgtggttg cgttcaatca
     6001 tgcggctgat tacttcgacc tgttgcgctt gcagtgaggc ttctgccagt gttatttgct
     6061 gcattactgc acccccttaa tttcatgata ttcagaacaa caaaggattc agtcattgac
     6121 gcatattgca tcatcacccg atgcagcaat cagccagcta cctgccttac ctgtaagctg
     6181 cttaatcagg gcgagcaatg actcaatctc accctcagtc attccacctg tgttacccat
     6241 catcagagaa caaataagtt ctgcctgcat tgcgcttttt gctgcttcat caacagtcat
     6301 atcggaaaaa tcacacatga attactcctc atcctgaata catgtaactg cgtggataat
     6361 tacgccggat aaaccggctt tagctgattt gttaattact gtgctgattg cagcctggac
     6421 gttttcggcc ttcacgttct gtgtgatgcc gatggtatgg ccgcgcttgt taacagcacg
     6481 ggaaaagacg cggaaggttt taagcatgag tcacctcctg acggatacgg gcgacaaaaa
     6541 tcagatgtgc gtgcggcagc cgggcgcgtg cttcgcgctc agtggcagcg gtaacggtga
     6601 atattgcaat gctgtctgtc aggcactgca taaagcgcca gacaaagtaa gggcgtgcgg
     6661 atacagccat gtgaaaggct cctctgactt tgggattata cccaccgccc aagaggccaa
     6721 tcttgctggc ggtggactgt acggaattgg ccttactggt gttagaggaa accagccttc
     6781 ccgaaggaag ttccatacag cccaccattg ttgaggtgtg cgtgtgcgcc gatacaaaaa
     6841 aaaagacgct ggcgcgtcgt gtatcgcctc taactccagc gggaggccaa tcccgatccc
     6901 cgttttataa gggatagcct cagtatatgc cgcttttttg cttaagtggt cggatgtgtt
     6961 gcgagagaag tttgtgcgaa aaatacctgc gttttctttg aaagcacata ataacatcac
     7021 gttatttatg tttatgcagt gtgtcgcaaa gcgacctgaa tcatcacgca aattacaacc
     7081 ataaaaaata cctgcgctgt gatatgaggt actacagcgc accgctttac gggtacagac
     7141 aatgacaaat gaagaattac aggaaatcgc attatccata cttgagcgac agcgcgaaaa
     7201 agcacaacgg caaaggaggt tgttacttaa tccactgcca ctcaccaggc caacacgatt
     7261 tgcccttgaa gattttgtcg agaaataccc acggcggtta aaagccacta aaaaccgacc
     7321 gcctggctga tagccttgtc caccagctac actcatggca tagttggtga tgcgcttgat
     7381 ttcctttatc tccattggcg acccttccgt ggtcgccttt gttttgcctg ccattcctgc
     7441 atcatgcgga tattcgcgcc atacagggcc accagcgcga taatttaata aaacctcctt
     7501 ctgataatgc ttaacgtgtt tcatgcgctc agcggcgaaa tggctcacga tatggtgcat
     7561 gtaatcagaa tggttgaggc tcagttgtgc gaacgtatcc gccgtgtcga tgtacagggc
     7621 ggcagcgttg cggatgtcag gcatgttcat gctctcccat gcgacgggcc agccagcgct
     7681 gtgaaaggcg gattaattcg gcttttcgct gctggtaagt catacccaac tcaatcagcg
     7741 tgatattgct ctggtcaagg taggaaaggt gttcaagctg cataacgctc atgctgtcac
     7801 gcggttttcc tttgatgccg ttcgcctgcg cccattgttt cgccgtcatg ccgcccagca
     7861 cgatacgcgc cagcatattt gattctgtgg tgtagtggtg ttgatgagtc tcttttccca
     7921 actctgcgcg gtacaactcc agcgcagcgc acataggctt aaaaaaactg gcaacggtga
     7981 cacgcgcttt cagttcacgg cgcaaagctg cggcacgttc cggcgctgcc ttgtgtagct
     8041 cttcttcaca tttgatgaag tactggcgaa ttgcgcggcc ctgttcagtg cgttcgacca
     8101 tcgccagttc tttagccatg ttcgtggaga tcaaatagtc ttcactgcgg cgatcaccgc
     8161 cgcgctttgt tgtccagcca gaatcaaact gttcaatatt tacactttgg ctcccccgtt
     8221 tcggggaatc aaagtacaaa ttttctacaa cctcaaaatc cacacctttt ttaaatccgt
     8281 actcctcaat acgccctttg atccaggtag tgaaatcacg gcctacaccg agcgctttat
     8341 gcagtgcgcg tgcgctgaca atgcccgttt catatccgcc aataatgcca ggtgataccg
     8401 gaataattgc ggcgaaatac tgttggctca gttcttcgga ctgaacgaag ccctgcccct
     8461 gtacgggcat attcggacgt ttcattgaaa actcctgctg tcgaatcagg tttggtgctg
     8521 acgggcgcgg aactgtacgc ccgtcatgct gatcactgct tggtgttgtt gcggcgctcg
     8581 ttcagccagt ggttgatctc caccgcatta aatacggtgt ttttctcccc cagcttcgtg
     8641 ggcttcggta atttgccgat acgcacccag cggtcgatag ttgacacgct ggtatcaagc
     8701 aacttagcca ggttgcagcg gcggatgtag cccgtggtag ggatagcttc ttgatggtta
     8761 gctgtcatga ttccctcttg ggttaaaagt tgatgttttc tctattctat tggttgtttg
     8821 tacagcattc catcaaatct ggaatctatc aaaatattca cagattctat tgaaataata
     8881 tatttccagt aaaagattgt gtgagcggga agaggtgcta agactttgaa agtttttgct
     8941 ttattggcga taggtcactg aatccgtata catcctgata ccgtgatata cccaatgcgt
     9001 cgttatgcgc tgttatggtt tgccatcttt tgccagtttt taacgtgtta atcctgttta
     9061 taccgattta taacgattaa taacggtgaa taaattttgc tggtggggga tgtatgggga
     9121 ttacagcccc gtgaatacag ggcgtgttgg ctggttaatc tgcctggctt actgccagta
     9181 atgcggctga tattttttgc ataaacgtgg atttggctag cccttttggc gatatccctt
     9241 tctccttcat cagcgcttcc atgtcgcggt aaatggctga acggtttggt tctccgtcct
     9301 gaatgtatgc ggttgtgcct gattttttca cgaggagtaa aaccagcaac cccatcatct
     9361 tcatgtttcc ctgttcttcg tcgagtttcc tgtgctgccc ccttccgctg cggcttgttt
     9421 ctttgatgta acggaccaga tcccgcccgc caagattaag ggctttttcc tcccagtttt
     9481 ttgtcccacg caaatcatcg atagtatctg ttaccgcctt gattattgcc tctggcgtgc
     9541 ctgcctccat gaacgggaag gaagcaagga atatatcccc cgctctgtat ggctcatcct
     9601 tgcttgtttt tactccacta tgcagcctga tagcacgtgc gatatgagtt ctggctgatt
     9661 cgacctgttc gaacttgtcc tgcggtacgt cgcctatcct cattgacgga ttaagatcag
     9721 ctaacgcaag ggccatttcg tggggagtga ttgaaacaga agcgcaaagg cgctctattg
     9781 gcgatttctt caggatgtta atcatgattt agcccttctg attggtgtca cgttgtagtg
     9841 ttctccgcgt tccagcgaca ctaacagagt ggtccacttc gccagtgcgt cccgtcgttc
     9901 attgaaatac tgatagcggt tgtatatgcc ttcgactccc ttgatgcggt gattgaggca
     9961 tctttcagcg actaccggat caacaccaag cgcagctaga tgtgtgcgtg ctgtacggcg
    10021 gaagtcgtga atagtgaaat ttggaacttc cggcatctct gcgcggactt ttgccagcgc
    10081 aacaggtagg gtgctctcct gaatgtgggg gatcatcctg tgctgcattt tccgcgccgg
    10141 aagcacccag gcactattgc atgacaacac acgcaactct cgcagccatt caataacggg
    10201 atggacgagt ggaatatcga tggggtcgcc gtttttgctg cgttcctccg gcagatgcca
    10261 tacagcgtta tcaaggtcga attcttccca tctggcggcg cacaactcca tcttcctgac
    10321 acaaagcgcc agcaatagct taaatgtgag ttcattctcg cggctgaaac cctttgcggt
    10381 ccgcatagcc tgaaagaatc ggattagctc ttcacgagtc agccaacggt cgcgggagga
    10441 ctccttgcca ccagcatcag agacttcaaa cgcagagcat gggttaatct ccagcacatg
    10501 acgttttacc ccgtagtcga atatgcggcg cgtccagcgt agaacgtccg ttgcaatggt
    10561 tggcgctcca cggtcaacaa ttgcctttag catctcgtca atgtgtcgtg gttttacgtc
    10621 ctcagccttc atatgaccaa tacgcgggtt tatgtctttg tcgatgcgac ggcgcaggat
    10681 gtcgggatgt ttccatcgtg gcagtatctg acgttcgaaa tattcggcag caagctctga
    10741 tacgcgcacg gccttttttt ctgcctcaat ttttgccagg gcttcggcct tacgctcctg
    10801 tttctctgct gctacgtcgt acccaagtgc gacgcgagcg gatagttcct ttgctgtttc
    10861 tctggctttt gatagcgagt aatcagcata ggaaccaatg accattgccc gtgcttttcc
    10921 tgcgaatttg tagcggaatc gccatgttgg tatctggtct tctttccggt aacgaaggta
    10981 taagccaccg ccatctgacc gcccttcgaa tcgttcacct gattttatcc atgccctgat
    11041 ctgaatatct gtgagctttg gcattaagaa ctcctgaaca ttgaatagag aggtgtaccc
    11101 aaacagatgt tccaaaatgc aattgggtac acctttgggt acacgctaat catgcgcttt
    11161 cttgcgtcgt tatgatagcc attaattaca aaaaaacccg cattatcgcg ggtttgattg
    11221 aattattgcg ctttattgat tccctctgat agcttactcc acattttgaa tctgctcgcg
    11281 catctgctca atcaacactt tcagctcaat cgcggagttt gtcacttcgg cattgataga
    11341 cttcgacgca agagtgttcg actcgcggtt gaattcctgc atcataaagt ccagacgacg
    11401 accaacggct tcttttttct tcagaatgtt gtaggtctct ttgacatgcg cttccaggcg
    11461 gtccagttct tcggcaacgt caattcgttg tgccagcaga accagctcct gctccagacg
    11521 gttgttttcc aactgaacct gagcatcttc cagcttcgcg accagacgct cacgctgcca
    11581 ttgcaggatt tccggcatat gggcgcggac tttgaccact tcggcggtga cgccttccag
    11641 acgctgctcg atcaatgctt tcagtgcctg accttcggtt tcgcgcgcga caataaagtc
    11701 gtccagcgta ccatccagcg ccgcgagaat ttcagcggca atggcgtcaa gatcctgctc
    11761 ctgggctgcc atcacgcccg gccagcgtag aatatcaacc gggttgattt ccccttcgtc
    11821 actctgcatt tttacccagt tcgcggcagt taccagctgt ttagccagtt tttcgttgag
    11881 gatcagctca ccttgcgcgc taacatctgg ctcatagcgc agggtacatt ccactttacc
    11941 gcgcgtcagg cgagaacgaa tacgctcgcg aacgacaggt tcaaggctac ggaactgctc
    12001 cggcagacga aagtaagttt ccagataacg ctggtttacc gagcgcattt cccaggttgc
    12061 gctcccccat tcacccttga tttcacgccg ggcgtaggcg gtcatactgc ggatcataga
    12121 cgttcctgtt tataaaagga gaggtggaag gattatagcc atcgatgcct tgtcaggata
    12181 ggaataaccg ccggaagtcc gtataatgcg cagccacatt tgtttcaagc cggagatttc
    12241 aatatgcgtc cagcaggccg tagcaataat caggtgcgtc ccgttaccct gactcgtaac
    12301 tatacaaaac atgcagaagg ctcggtgctg gtcgaatttg gcgataccaa agtgttgtgt
    12361 accgcctcta ttgaagaagg cgtgccgcgc ttcctgaaag gtcagggcca gggctggatc
    12421 accgcagagt acggcatgct gccacgttct acccacaccc gtaacgctcg tgaagcggcg
    12481 aaaggtaagc agggtggacg cacaatggaa atccagcgtc tgatcgcccg tgctcttcgc
    12541 gcggcagtag atttgaaagc gctgggtgag ttcaccatta cgctggactg cgacgtgctt
    12601 caggctgatg gtggcacgcg taccgcgtcg attacgggtg cctgcgtggc gctggcagat
    12661 gcgctacaga agctggtgga aaacggcaag ctgaaaacca acccgatgaa agggatggta
    12721 gccgcagttt ctgtcggtat tgtaaacggt gaagcaattt gcgatctgga atacgttgaa
    12781 gactcagccg cagagaccga catgaacgta gtgatgaccg aagacgggcg catcattgaa
    12841 gtgcagggga cggcagaagg cgagccgttc acccatgaag agctactcac cttgttggcg
    12901 ctggcccgag ggggaatcga atccattgta gcgacgcaga aggcggcgct ggcaaactga
    12961 tttttaaggc gactgatgag tcgccttttt tttgtctgta gaaaagtaag atgaggagcg
    13021 aaggcatgaa accatatcag cgccagttta ttgaatttgc gcttagcaag caggtgttaa
    13081 agtttggcga gtttacgctg aaatccgggc gcaaaagccc ctatttcttc aacgccgggc
    13141 tgtttaatac cgggcgcgac ctggcactgt taggccgttt ttacgctgaa gcattggtgg
    13201 attctggcat tgagttcgat ctgctgtttg gccctgctta caaagggatc ccgattgcga
    13261 ccactaccgc cgtggcgctg gcggagcatc atgaccttga cctgccgtac tgctttaacc
    13321 gcaaagaggc aaaagaccac ggtgaaggcg gcaatctggt tggtagcgcg ttacaaggac
    13381 gcgtaatgct ggtagatgat gtgatcaccg ccggaacggc gattcgcgaa tcgatggaga
    13441 ttattcaggc taatggcgcg acgcttgctg gcgtattgat ttcgcttgat cgtcaggaac
    13501 gggggcgcgg cgagatttcg gcgattcagg aagttgagcg tgattacaac tgcaaagtga
    13561 tctctatcat caccctgaaa gatctgattg cctatctgga agagaagccg gaaatggcgg
    13621 aacatctggc ggcggttaag gcctatcgcg aagagtttgg cgtttaaaga aactcgccgg
    13681 atgaaaagtc atccggcgtc atattactgc aactgcgcgg caattagcgg ccagcgggcg
    13741 tcaaaatcat ccgtcgggcg gtatttaaat tcgctgcgga caaaacgtga cagcatacct
    13801 tcacagaagg ccaggatctg gcttgccagc agggtttcat cggtggtgta accttcaccc
    13861 tcacgcattc tcttttcacg caatacctga cgcagctgcg cttcaatacg ctcgaacagc
    13921 tggttgatac gcccttgcag gcgatcctgt tcaaacatca gcgcatgacc agtgaggatg
    13981 cgggtcaggc caggattacg ctcaccaaaa ccgagaagca gcaacacaat cagacgcagg
    14041 cgcgctgtgg tgtctttctc atctttcaga atcaggttga tgcgagtaat caggctatct
    14101 tcgataaact caatcaggct atcgaacatg cgggtcttac tggggaagtg gcgatacagt
    14161 gccgcttcgg aaacgccgac agaggcggcc agttttgccg tcgtgatacg ttggcttcca
    14221 tcgctggatt ccagcatcag cgccagagac tgaagtattt cctcgcgacg gttccttttc
    14281 gcagtttgtt tttctgccat gttacaaaat acccctgaaa ataagcactt gccaggcggc
    14341 acccacgcta tgaccgcaaa cgaaatgttt gcggctatgt tatgacgtta ttcggatgcg
    14401 tatgtgttac tgacgaccag agtgaccaaa gccgccttca ccgcggtcgg tggcgtcgaa
    14461 atcttccacc agattaaatt cagcctgtac taccggaaca aaaatcatct gggcgatgcg
    14521 ttcgccaggt tgaatggtga agctgtcctg accacggttc cacacggaaa tcatcaactg
    14581 gccctgatag tcggaatcga tcaatcctac caggttacca agcacgatac cgtgcttatg
    14641 tcccaatccg gagcgcggca gcatcattgc cgccagtgaa ggatcggcaa tatgaatcgc
    14701 cagcccggtc ggaaccagcg tagtgtcacc cggagccagt tctacggcgt cgtcgagaca
    14761 ggcacgcagg tcaagtccgg cagagccaga ggtggcataa gtcgggagcg gaaattcctt
    14821 cccaacgcgc gggtccagaa tcttaacgtc gatttttttc atcataacgg gtcacgatct
    14881 cgtcgagtaa taattggcca aggagctctt tgcgctcaag cggtaagact ttatctccgt
    14941 cctgccagaa aaggtgtaat gcgttgttgt cgctgttaaa tccttgagtt ggctgggaaa
    15001 catcgttcgc gcagatcaga tcaaggtttt tacggatacg tttttgccgg gcgtattctt
    15061 ccacattatt tgtttcggcg gcaaatccaa cgacgtaggg tcgatggtct tttagtgcgg
    15121 caacgcctgc gacgatatcg gggtttttaa ccatttttat tgttaattca tcaccctgcg
    15181 tggcctgctt tttgattttc tctggggcca cggtagctgc gcgataatcc gccacggcgg
    15241 cgcaaccgat aaaaatattt tgctgctgta cagaagcatt cacggcggct tccatttcca
    15301 gcgcagtcat cacatcaaca cgtttaacaa acggtggcgt cggtagtgaa accggacctg
    15361 ataccagcgt gacgttcgcg ccacgacggg cagcggcggc ggcgatagca aaacccatct
    15421 tgccggagct gtgattagag atataacgca ccggatcgag cggttcacgc gtcgggccgg
    15481 cggtaatcat aatgttcaga tgtttcaggt cgttgacggg cgaaaaatgc gccaccgcca
    15541 tatccacaat ggttaacgga tcgagcattc gcccaggacc gatatcacca caagcctgac
    15601 tgccactgtc tggcccccag atgagcatac cacgggaagc aagcacctct aaattatgct
    15661 gcgtggcagc ggcacggtac atctgctggt tcatggcggg gagcacggct acaggcgcag
    15721 gtgtagccag acaaatcgtc gataccaggt cattcgccat tccggcagca atacgggcaa
    15781 tcaaatctgc cgtggcaggg gcgagaatca ctaaatcagc ccatttaccc agctcaatat
    15841 ggcccatagc ggcttcggct gccgggtcca gcagactgtc ggaaacggga taaccagaaa
    15901 ccgcctgcaa gctaagtggg gtgataaagg cttttgccgc ttcggtcatg gctacgcgga
    15961 cgtcggcccc gcgatcgcgc aaacgacgca ccagttcagg ggttttatag gcagcaatac
    16021 cgccgctaac gccgagaacg atttttttac cggccaggct catgatgatt tttcctgtag
    16081 ggtgacacca gaagttggcg attttatcac aatacttttg ttgtcgtgcc ttcacctgag
    16141 attcactttg cgaggcgctt tccaggattg aaaactggcc gtcgatttaa cgaaacggct
    16201 atgacaggat gcgagcacca caaaggaggt gaaggtgaaa aacaattcac agctgttgat
    16261 gccgcgcgaa aaaatgctga agtttggtat tagcgcctta acggatgtcg agctgctggc
    16321 gctatttctg cgtaccggaa cgcgcggtaa agatgtatta accctggcaa aagagatgct
    16381 ggagaatttc ggctctcttt atggcttgtt aacctctgaa tatgaacaat ttagtggcgt
    16441 tcatggaatt ggcgtggcga aatttgccca gttaaagggg attgctgaac tggcgcggcg
    16501 ttactacaac gtgcggatgc gtgaagaaag ccctttactc agcccggaga tgacgcggga
    16561 atttttacaa agccagctca cgggtgagga gcgggagatc tttatggtga tctttctcga
    16621 ctcccaacac cgggttataa cgcatagccg tcttttttcc ggcacgctaa accatgttga
    16681 agtccatcct cgggaaatta tccgcgaagc gataaaaata aacgcctcgg cgctgatcct
    16741 tgcacataat cacccttcgg gttgtgctga acccagtaaa gcggataaac tcattactga
    16801 acggataata aagagttgtc agttcatgga tttacgcgtg ctcgaccata tcgtgattgg
    16861 gcgtggagag tatgtttctt ttgccgaacg cggctggatt taacccgcta tgcgcgatcc
    16921 ttcgggatct ttgtctgttc gggacttgag cacatcgctg agtcagcgta tactacgcca
    16981 cctttgagaa tctcgggttt ggcatttggg cctggcaatc gagagttcac atagaactgc
    17041 gatgaccggg ctgtaaagcc tgacgaggcg ccaatacccc atacgaagct cgagctaatt
    17101 tgatttttgg agaatagaca tgtcccgagt ctgccaagtt actggcaagc gtccggtgac
    17161 cggtaacaac cgttcccacg cactgaacgc gactaaacgc cgtttcctgc cgaacctgca
    17221 ctctcactgt ttctgggttg agagcgagaa gcgttttgtc accctgcgcg tatctgctaa
    17281 aggtatgcgt gtaatcgata aaaaaggcat cgatacagtt ctggctgaac tgcgtgcccg
    17341 tggcgaaaag tactaagtac ttagaggaaa taaatcatgg ctaaaggtat tcgtgagaaa
    17401 atcaagctgg tttcttctgc tggtactggt cacttctata ccactacgaa gaacaaacgt
    17461 actaagccgg aaaaactgga actgaaaaaa ttcgatccag ttgttcgcca gcacgtgatc
    17521 tacaaagaag cgaaaatcaa ataattctcg ctttgatgta acaaaaaacc tcgctccggc
    17581 ggggtttttt gttatctgct tgcccccata ttgactgcat ctgttcattc ctggagatgc
    17641 tatgcctgaa ttacccgaag ttgaaaccag ccgccgcggc atagaaccgc atctcgttgg
    17701 tgcaaccatt cttcatgcgg tggtgcgcaa cggacgcttg cgctggccgg tttcagaaga
    17761 gatctaccgt ttaagcgacc aaccagtgct tagcgtgcag cggcgggcta aatatctgct
    17821 gctggagctg cctgagggct ggattatcat tcatttgggg atgtctggca gcctgcgcat
    17881 ccttccagaa gaacttcccc ctgaaaagca tgaccatgtg gatttggtga tgagcaacgg
    17941 caaagtgctg cgctacaccg atccgcgccg ctttggtgcc tggctgtgga ccaaagagct
    18001 ggaagggcac aacgtgctgg ctcatcttgg accggagccg cttagcgacg atttcaatgg
    18061 tgagtatctg catcagaagt gcgcgaagaa aaaaacggcg attaaaccgt ggctgatgga
    18121 taacaagctg gtggtagggg tagggaatat ctatgccagc gaatcactgt ttgcggcggg
    18181 gatccatccg gatcggctgg cgtcatcact gtcgctggca gagtgtgaat tgttagctcg
    18241 ggtgattaaa gcggtgttgc tgcgttcgat tgagcagggt ggtacaacgc tgaaagattt
    18301 tctgcaaagt gatggtaaac cgggctattt cgctcaggaa ttgcaggttt acgggcgaaa
    18361 aggtgagccg tgtcgggtgt gcggtacgcc gattgtggcg actaaacatg cgcagcgggc
    18421 aacgttttat tgtcggcagt gccagaagta attcatgcgc gccggatggc ataccatccg
    18481 gcataaacgc tacgctaact tcgccatcag cgcctgatgg acattctccg gcaggaaatg
    18541 ggtgacatcg ccctgatggc gtgccacctc tttcaccaac gatgaagaga taaacgacca
    18601 ctctttcgac ggcatcagaa acacactttc cagttccggc attaagtggc gattcatatg
    18661 cgccagctgc atttcatatt caaaatccgc caccgcacgc aggccacgaa tcagcaccgt
    18721 agcgtgttga ttacgggcga agttcgccat caaatcacta aacccgacca cttccacgtt
    18781 ccccagatgc gcggttgcct gctgtgccag tgccacacgc tcttccaggg taaacatcgg
    18841 ttttttactg gggctggcgg caatcgccag aataacgtga tcgaacatct gcgtggcgcg
    18901 cgtcacgata tcgatatgac cattggtaat gggatcgaaa gtacccggat aaatcgcccg
    18961 tttttgcata acaacctcaa tgcgttttcg gtggcaggta aggttccagc agttgaagca
    19021 gacgctgtag cgcgccctgg ttttgataca gtacttcaac ggcatgacgg ccatagaaac
    19081 tacggtaatc ggcgtcggtg agtaaagagg aaacctcttt tgcaagcgta gtggcatcgg
    19141 taacggtaat aagcccgctt gcctgctcca gccgcgcgca aatgtcttta aagttaaaag
    19201 tatgcggccc catcaatacc ggaatagcgt gtgcggcagc ttccagcgga ttatgcccac
    19261 cacgttcaac cagtgaaccg ccaacaaagg cgagatcggc aatgccgtac agtaacatca
    19321 actcgcccat cgtatcgcca accacaacct gcgtgctggt ggaggggact tcccctgaag
    19381 agcgtgtgat atagcttagt ccagcctggc ggacaaggtt aatcgcatcc gggaagcgtt
    19441 ctggatgacg gggtaccagg atgagcaata aattcgggaa ttgctgtaac aatgcctgat
    19501 gtgcggcaat gaccacgctc tcttcacctt cgtgagtgct ggtggcaatc cataccgggc
    19561 ggtgtggtgc ccattggcgg cgtagcgtca cggctttagc agccaactgc ggcgttacag
    19621 aaatatcgaa tttcaggcta ccggtaacgg ttacctggtt attttttgcg cccagcgcca
    19681 caaaacgtgc accatcttct tcattttgcg cagcaatcag cgtaatacga cgcagcaagc
    19741 ggcggacgaa tttacccagt ttggcataac ctgcggccga gcgggcagag agtcgcgcgt
    19801 tagcgatcac cagcggaatt ttacgtttat gtagcgccgc aatcaggtta ggccatagtt
    19861 cggtttccat aatcaacacc agtttggggt cgactttatt caggaaacgg ttgagcgcat
    19921 cgggcaggtc atacggcaga taaacgtgct gaacatcctt cccgaaagcc gattgtacgc
    19981 gctccgaacc ggttggcgtc atggttgtaa cggtaatcgg taaatcagga taacgatgac
    20041 gcagcgcgcg caccaacggg atcgccgcca gagtttcacc gacggagacg gagtgcagca
    20101 taatgccgcc tggttttagc ggatggcggt aaaaaccgta acgttcaccc cagcgttttc
    20161 gataggccgg agccttacgt ccgcgcaccc agagccgtat ccagatcagc ggctgaataa
    20221 ggtagagaag ggcggtgtaa agcaattcga gcatagtaaa tagctgactt atggatgtgc
    20281 tggggattct atgtatttag ctgtggcttt accattactt ttcccgtttt tgacttaaat
    20341 agcaatttac ccgccaattc ttgctgaaca tctatacttt ttttgtattt ttatgcgatt
    20401 cattgatttc tgctgtacgt gcaggaagga atcgcagagc aacagtgaaa gaatatggtt
    20461 gctaatgatt ttatttgatt gatttcttta ttgaaaatca acaatgtgct taaaaattat
    20521 tcgggtcgga ttgccgcgaa agaagtgata cactagcgcg tcaatatcag tgcggtatca
    20581 gtattcaggt agctgttgag cctggggcgg tagcgtgctt tttctgcttc accttaatcg
    20641 gataatctca acaaaaagag ttacttgtgg ataagccatt tcgaagaatt ttgctcatta
    20701 agatgcgttt tcatggggat atgttattaa ctactcccgt cattagttcg ctgaaaaaaa
    20761 attaccctga cgcaaaaatc gatgtgctgc tttatcagga caccatcccg atcctgtctg
    20821 aaaatccaga gattaacgcg ctctacggca taaaaaataa aaaagcaaaa gcctcagaaa
    20881 aaattgccaa cttttttcat ctcatcaagg tattacgtgc caataagtat gaccttatcg
    20941 tcaatctcac cgatcaatgg atggttgcta tactggttcg cttattaaat gcccgtgtga
    21001 aaatttccca ggattatcat catcggcagt ctgctttttg gcgtaaaagt ttcacccatt
    21061 tggtgccgtt gcagggtgga aatgtggtgg aaagtaactt atccgtgctg accccattgg
    21121 gagttgattc gttggtgaag cagacaacca tgagttaccc gcctgcaagc tggaaacgta
    21181 tgcgtcgcga acttgatcac gctggtgttg gacaaaatta tgtggttatc caacctacgg
    21241 cgcggcaaat cttcaaatgc tgggacaacg ccaagttttc cgctgtgatt gatgccttac
    21301 atgctcgtgg ttatgaagtt gttctgacgt ccggcccaga taaagacgat ctggcctgcg
    21361 tcaatgaaat tgcgcaggga tgccagacgc caccagtaac ggcgctggct ggaaaggtga
    21421 ccttcccgga acttggtgcg ttaatcgatc atgcgcagct gtttattggc gttgattccg
    21481 caccggcgca tattgccgct gcagttaata cgccgctgat atcgctgttt ggtgcgacag
    21541 accatatttt ctggcgtccc tggtcaaata acatgattca attctgggcg ggagattacc
    21601 gggaaatgcc aacgcgcgat cagcgtgacc gaaatgagat gtatctttcg gttattccgg
    21661 cggcagatgt cattgctgct gtcgataaat tactgccctc ctccacgaca ggtacgtcgt
    21721 tatgatcgtt gctttttgtt tatataaata ttttcccttt ggcggtttgc agcgtgattt
    21781 tatgcgtatt gctcagacag tcgccgcccg aggtcatcat gttcgggttt atacccagtc
    21841 gtgggaaggc gaatgccctg atgtatttga actgatcaaa gtgccggtta aatcgcatac
    21901 caatcacggg cgcaatgcgg agtattttgc ctgggtgcaa aaacatttac gcgaacatcc
    21961 cgtcgataaa gtcgttggat tcaacaaaat gccggggctg gacgtttatt atgccgctga
    22021 tgtttgttat gccgagaaag tagcgcagga aaaaggcttt ttctatcgcc tgacgtcacg
    22081 ttatcgccat tatgccgcct ttgagcgggc aaccttcgaa cagggtaagc cgacacagtt
    22141 gctgatgctg acagataagc aaatcgccga tttccagaaa cattatcaga ctgaagcgga
    22201 gcgttttcat attctgccac cggggattta tcctgatcgt aaatatagcc agcagccagc
    22261 caatagccgt gaaatcttcc gtaagaagaa tggaataacc gaacaacaat atttattgtt
    22321 gcaggtcggt tcagacttca cgcgtaaagg tgtcgatcgt tccattgaag cacttgcttc
    22381 gttaccggat tcgctgcgcc acaacacatt gctatatgtt gttgggcagg ataaaccgcg
    22441 aaaatttgag gcgctggcag aaaaacgcgg cgtgcgcagt aatgttcact ttttctcggg
    22501 gcgcaatgat gtctcggaat taatggcggc ggcggattta ttactgcatc ctgcctacca
    22561 ggaagcggcg ggaattgtgc tgctggaagc gattactgca ggattaccgg tactaacaac
    22621 agccgtttgt ggctatgcgc attatattgt cgacgctaat tgcggcgagg ctattgctga
    22681 gccattccgc caggaaacat tgaatgagat tttacgcaaa gcgttaacgc aatcttcatt
    22741 gcgccaggcc tgggcggaaa atgcgcgaca ttatgctgat acacaagatt tatacagtct
    22801 gccagagaaa gcggcggata tcataacggg tggtctggat ggttgaactt aaagagccgt
    22861 ttgccacgtt atggcgcggc aaagatcctt ttgaggaagt taaaaccttg cagggtgagg
    22921 tatttcgtga actggaaact cgccgtactc tgcgctttga aatggcgggc aaaagctatt
    22981 ttctcaaatg gcatcgcggc acgaccctga aagagataat caaaaattta ctctcattgc
    23041 ggatgccagt attaggcgct gaccgcgaat ggaatgcgat tcatcgactg cgggatgtcg
    23101 gcgttgatac tatgtatggg gtggcatttg gcgaaaaagg catgaatccg ctgaccagaa
    23161 cttcatttat tattaccgaa gatctgacac caaccataag tcttgaagat tactgtgctg
    23221 actgggcgac taaccctcca gatgttcgcg taaagcgtat gcttattaag cgtgtcgcga
    23281 cgatggtgcg cgatatgcat gctgcgggca ttaaccaccg tgactgttat atctgtcatt
    23341 tcctgctgca cttgcctttt tccggtaagg aagaggagtt aaaaatttcg gtaattgacc
    23401 tgcaccgggc gcagcttcgc acgcgcgttc cacgtcgttg gcgggataaa gatcttattg
    23461 ggctttattt ttcttcgatg aatatcggcc tgactcagcg ggatatctgg cggtttatga
    23521 aagtgtattt tgccgccccg cttaaagaca ttctcaagca ggaacaagga ctgctgtcgc
    23581 aagcagaagc aaaagccaca aaaatcaggg aaagaacgat tcgaaaatcg ttgtaacccg
    23641 taacttattt ttgccaaaat tttggataca gaataaatat gtctcaactc aatgatagtg
    23701 acatcatcct ttttgagtat aattttcatt atcaaaatat aagatctaaa aatactcttg
    23761 atatagcatt tggtattgac agaaattttc tttttggatg tggtgtagcc atcgcatcta
    23821 ttctattaaa caatagagaa atctc
//
LOCUS       DAZKIV010000065        23752 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZKIV010000065 DAZKIV010000000
VERSION     DAZKIV010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.065"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            110..613
                     /gene="ftnB"
                     /locus_tag="ACHOT6_002487"
     CDS             110..613
                     /gene="ftnB"
                     /locus_tag="ACHOT6_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFP9947687.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(692..943)
                     /gene="yecJ"
                     /locus_tag="ACHOT6_002488"
     CDS             complement(692..943)
                     /gene="yecJ"
                     /locus_tag="ACHOT6_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFP9947688.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1058..1144)
                     /gene="azuC"
                     /locus_tag="ACHOT6_002489"
     CDS             complement(1058..1144)
                     /gene="azuC"
                     /locus_tag="ACHOT6_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFP9947689.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1408..1731
                     /gene="yecR"
                     /locus_tag="ACHOT6_002490"
     CDS             1408..1731
                     /gene="yecR"
                     /locus_tag="ACHOT6_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFP9947690.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            1902..2399
                     /gene="ftnA"
                     /locus_tag="ACHOT6_002491"
     CDS             1902..2399
                     /gene="ftnA"
                     /locus_tag="ACHOT6_002491"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFP9947691.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2437..2676)
                     /gene="yecH"
                     /locus_tag="ACHOT6_002492"
     CDS             complement(2437..2676)
                     /gene="yecH"
                     /locus_tag="ACHOT6_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFP9947692.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIHSEEGFSTDQSKICRH"
     gene            2867..4078
                     /gene="tyrP"
                     /locus_tag="ACHOT6_002493"
     CDS             2867..4078
                     /gene="tyrP"
                     /locus_tag="ACHOT6_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFP9947693.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4140..4805)
                     /gene="yecA"
                     /locus_tag="ACHOT6_002494"
     CDS             complement(4140..4805)
                     /gene="yecA"
                     /locus_tag="ACHOT6_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFP9947694.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5001..5087)
                     /locus_tag="ACHOT6_002495"
     tRNA            complement(5001..5087)
                     /locus_tag="ACHOT6_002495"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5051..5053),aa:Leu,seq:taa)
     gene            complement(5100..5173)
                     /locus_tag="ACHOT6_002496"
     tRNA            complement(5100..5173)
                     /locus_tag="ACHOT6_002496"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5139..5141),aa:Cys,seq:gca)
     gene            complement(5228..5303)
                     /locus_tag="ACHOT6_002497"
     tRNA            complement(5228..5303)
                     /locus_tag="ACHOT6_002497"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5268..5270),aa:Gly,seq:gcc)
     gene            complement(5455..6003)
                     /gene="pgsA"
                     /locus_tag="ACHOT6_002498"
     CDS             complement(5455..6003)
                     /gene="pgsA"
                     /locus_tag="ACHOT6_002498"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFP9947695.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6060..7892)
                     /gene="uvrC"
                     /locus_tag="ACHOT6_002499"
     CDS             complement(6060..7892)
                     /gene="uvrC"
                     /locus_tag="ACHOT6_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFP9947696.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(7889..8545)
                     /gene="uvrY"
                     /locus_tag="ACHOT6_002500"
     CDS             complement(7889..8545)
                     /gene="uvrY"
                     /locus_tag="ACHOT6_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFP9947697.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8687..8758
                     /locus_tag="ACHOT6_002501"
                     /pseudo
     CDS             8687..8758
                     /locus_tag="ACHOT6_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8841..9017
                     /gene="yecU"
                     /locus_tag="ACHOT6_002502"
     CDS             8841..9017
                     /gene="yecU"
                     /locus_tag="ACHOT6_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFP9947698.1"
                     /translation="MIKIFIGHYINAFNSTVDDTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9004..9228
                     /gene="yecF"
                     /locus_tag="ACHOT6_002503"
     CDS             9004..9228
                     /gene="yecF"
                     /locus_tag="ACHOT6_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFP9947699.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9295..10017)
                     /gene="sdiA"
                     /locus_tag="ACHOT6_002504"
     CDS             complement(9295..10017)
                     /gene="sdiA"
                     /locus_tag="ACHOT6_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFP9947700.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLSNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(10247..10999)
                     /gene="tcyN"
                     /locus_tag="ACHOT6_002505"
     CDS             complement(10247..10999)
                     /gene="tcyN"
                     /locus_tag="ACHOT6_002505"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFP9947701.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(10996..11664)
                     /gene="tcyL"
                     /locus_tag="ACHOT6_002506"
     CDS             complement(10996..11664)
                     /gene="tcyL"
                     /locus_tag="ACHOT6_002506"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFP9947702.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(11679..12665)
                     /gene="dcyD"
                     /locus_tag="ACHOT6_002507"
     CDS             complement(11679..12665)
                     /gene="dcyD"
                     /locus_tag="ACHOT6_002507"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFP9947703.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(12770..13570)
                     /gene="tcyJ"
                     /locus_tag="ACHOT6_002508"
     CDS             complement(12770..13570)
                     /gene="tcyJ"
                     /locus_tag="ACHOT6_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9947704.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(13658..14209)
                     /gene="fliZ"
                     /locus_tag="ACHOT6_002509"
     CDS             complement(13658..14209)
                     /gene="fliZ"
                     /locus_tag="ACHOT6_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFP9947705.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCSGLVQKSSSLPASDIY"
     gene            complement(14255..14974)
                     /gene="fliA"
                     /locus_tag="ACHOT6_002510"
     CDS             complement(14255..14974)
                     /gene="fliA"
                     /locus_tag="ACHOT6_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFP9947706.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(15294..16625)
                     /locus_tag="ACHOT6_002511"
     CDS             complement(15294..16625)
                     /locus_tag="ACHOT6_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliC/FljB family flagellin"
                     /protein_id="HFP9947707.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSTSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISLAQTTEGALSEINNNLQRVRELTVQATTG
                     TNSDSDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLSKDGSMKIQVGANDGQTISIDL
                     KKIDSSTLGLNGFSVSKNSLNVSNAITSIPQAAGNEPVDVNFGDTDESAAIAAKLGVS
                     DTSSLSLHNILDKDGKATADYVVQSGKDFYAASVNAASGKVTLNTIDVTYDDYANGVD
                     DAKQTGQLIKVSADKDGTAQGFVTLQGKNYSAGDAADILKNGATALELTDLNLSDVTD
                     TNGKVTTTATEQFEGASTEDPLALLDKAIASVDKFRSSLGAVQNRLDSAITNLNNTTT
                     NLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLSKANQVPQQVLSLLQG"
     gene            16871..18286
                     /gene="fliD"
                     /locus_tag="ACHOT6_002512"
     CDS             16871..18286
                     /gene="fliD"
                     /locus_tag="ACHOT6_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFP9947708.1"
                     /translation="MASISTLGVGSGLDLSSILDSLEAAEKSTLTPISKQQSSYTAKL
                     SAYGTLKSALESFQTANTALNKADLFTATSTTSSSSAFSATTTGSAIAGKYTISVSQL
                     AQAQTLTTKISQKDSKAAIATSDSVLTIQQGGGKDPVTIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSITANDTGNDNAMKLSVSGDSALESFMGYNGTSGDSSNGM
                     IESVTAQNAKLTVNNVEIENSSNTISDALEDITLNLNDVTTGNQTLTISKDTSKAENA
                     VKAWVDAYNTLQDTFSSLTKYTAVDAGAESQDSSNGALLGDSTLRTIQTQLKTLLANT
                     HSSSNYKTLAQIGITSDASTGKLEIATDKLQTALKKDAAGIGEMFIGDGKSTGVTTGI
                     SSNLTSWLSSTGIIQAAKDGVSKTLNNLTDQYNAASERIDTLMTRYKAQFTQLDVLMN
                     SLNSTSSYLTQQFDTSNSNSK"
     gene            18302..18712
                     /gene="fliS"
                     /locus_tag="ACHOT6_002513"
     CDS             18302..18712
                     /gene="fliS"
                     /locus_tag="ACHOT6_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFP9947709.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEKSKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            18712..19077
                     /gene="fliT"
                     /locus_tag="ACHOT6_002514"
     CDS             18712..19077
                     /gene="fliT"
                     /locus_tag="ACHOT6_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFP9947710.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVDPSTTMQEQIRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            19155..20642
                     /gene="amyA"
                     /locus_tag="ACHOT6_002515"
     CDS             19155..20642
                     /gene="amyA"
                     /locus_tag="ACHOT6_002515"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9947711.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(20676..21089)
                     /gene="yedD"
                     /locus_tag="ACHOT6_002516"
     CDS             complement(20676..21089)
                     /gene="yedD"
                     /locus_tag="ACHOT6_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFP9947712.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            21276..22481
                     /gene="yedE"
                     /locus_tag="ACHOT6_002517"
     CDS             21276..22481
                     /gene="yedE"
                     /locus_tag="ACHOT6_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFP9947713.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQALRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            22478..22711
                     /gene="yedF"
                     /locus_tag="ACHOT6_002518"
     CDS             22478..22711
                     /gene="yedF"
                     /locus_tag="ACHOT6_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFP9947714.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            22820..23491
                     /gene="yedK"
                     /locus_tag="ACHOT6_002519"
     CDS             22820..23491
                     /gene="yedK"
                     /locus_tag="ACHOT6_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFP9947715.1"
                     /translation="MCGRFAQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(23531..>23752)
                     /locus_tag="ACHOT6_002520"
     CDS             complement(23531..>23752)
                     /locus_tag="ACHOT6_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9947716.1"
                     /translation="TSQRCACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAA
                     GHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         5897 a         6021 c         5748 g         6086 t
ORIGIN      
        1 ctccacgatc acatttcgga ccggcagaaa ggaattattc tacaaacagt aattatggtg
       61 ttttgattta tcttgcacct ctccacttct ggatataagg atattaggta tggcaaccgc
      121 tggaatgctt ctcaaactca actctcaaat gaaccgcgag ttttacgcat ccaatctcta
      181 ccttcacctg agtaactggt gttctgaaca gagtctgaac ggcaccgcca ctttccttcg
      241 cgcccaggca cagagtaatg tgacccaaat gatgcgcatg tttaacttta tgaagagtgt
      301 cggcgctacc cccatcgtta aagccattga tgttcccggt gaaaaactga actctctgga
      361 agaactgttc caaaaaacga tggaagaata cgagcaacgt tcaagcacgc tggcgcagtt
      421 agccgatgaa gcgaaagaac tgaatgatga ttcaaccgtc aatttcctgc gcgatcttga
      481 aaaagaacag cagcatgatg gcctgttgct gcaaaccatt cttgatgaag tgcgcagtgc
      541 gaaacttgcg ggtatgtgcc ctgtgcagac cgaccaacat gttctgaatg tcgtgtcaca
      601 ccagctgcat tgatcatcat cggcgctaat gcattgcgcc gatgaaggtt ttgagaaacc
      661 gctgcctcat ctgtttgaag cagcggtttt tttaatggga ttcaccctgt ggggtaaact
      721 tgagttcaat aagcgcgatg gctttttgga ttgcccgcat ggtgaccggg tctgcggcgg
      781 cgggatggtt agtaaagtcg atattcttca gctgactgga cattttttca cgaacttcaa
      841 cgggcgcgat tacatcgaga acatccagaa tttgtttgat aaccaactgg caagcaacca
      901 catcagaaac caattcctga tcggcattca gcggctggga catcgtaaac tcctgatagc
      961 attttgaaag ccgttatagt agcggcttca catcttcagc gatagtcaca tccaccgtca
     1021 tcaggagaca aaaaaacctg ccggagcagg ttttttgtta tcggaacata ttgcctggcg
     1081 gtacgtcttt gaacgtcttg caatagttat tgaacatact tttcaggatt ttgcgcagtt
     1141 tcatcgcggc actccgagca tttgttatac aggtgttatt gtctttgcac ttataatatg
     1201 acaaccatca caaaaatcaa tctttatgtg atatgaatca cataaatacc ccctttaatg
     1261 ttataaaaat gataatcaaa aagcagcccc cccatttctg acacctacag atggcaagaa
     1321 atagcgcctg ccaggcgtct tttccggcca ttgtcgcagc gctgtaacgc gtaaaatagt
     1381 gctttctctt actcttctgg ctggaccatg agacttctga ttctgactct ttcattaata
     1441 acgctggcgg ggtgtacggt tactcgccaa gcccatgtga gcgaagttga cgcggcaaca
     1501 ggtattgtac ggttggttta tgaccaggct tttttgcagc atgcccatac cgatcgttat
     1561 gtcagtcgcg ggatcgccga tcgcgcatgc cagcaggaag gctataccca cgcgatcccc
     1621 tttggtcagc cagtaggcaa ctgtagcctt tttgcgggtt ccttatgttt gaatacggaa
     1681 ttcactttgt catatcagtg ccatcattcc gccttccctg tctttctcta aatattttaa
     1741 tcagcgaggg gatcttcgct gattaaataa atagcggtaa tgcgttttat tcccattcgt
     1801 atttttaata attgaagttt atattttacc ttttgcaaat aataaaataa caaattatag
     1861 tgacgccaca gcaacaaata taacctttgt ggagcactat catgctgaaa ccagaaatga
     1921 ttgaaaaact taatgagcag atgaacctgg aactgtactc ttcactgctt tatcagcaaa
     1981 tgagcgcctg gtgcagctat cataccttcg aaggtgctgc cgcgttcctg cgccgtcacg
     2041 cccaggaaga gatgacgcat atgcagcgtc tgtttgatta cctgactgat accggcaatt
     2101 taccgcgtat taataccgtt gaatctccgt ttgctgaata ttcctcactt gatgaattat
     2161 tccaggaaac ctataaacac gaacaattaa tcacgcagaa aattaacgaa ctggctcatg
     2221 ctgcaatgac caatcaggac tacccaacat ttaatttcct gcaatggtat gtttctgagc
     2281 agcatgaaga agagaaactg ttcaaatcga ttattgataa attaagcctg gcaggcaaaa
     2341 gcggcgaagg tctgtatttt atcgacaaag aactctctac cctcgacaca caaaactaat
     2401 gctctcaggc ggcaggtttt ctgccgccgt ttcaaatcag tgacggcaaa tcttactctg
     2461 atcggtcgaa aaaccttctt ctgaatgtat aaatttgcct tttgctgcca gaaacgctac
     2521 cagctctccc gcagtcatcc cttctgccga gcaggtgtga aaacgtgcct gttcaccaaa
     2581 acgcgcttta attgctgctt ccagactggc atgcgtatat tgctcgcctg attcaatcat
     2641 catatttaac acttcatgac cgtgaataga gtccatcgtt cctcctcaaa aaaagcctag
     2701 cgtagcgatt gccgcttatg aagactttgc gccagagcag aactgaatgc tttttattgt
     2761 acatttatat ttacaccata tgtaacgtcg gtttgacgaa gcagccgtta tgccttaacc
     2821 tgcgccgcag atatcactca taaagatcgt caggacagaa gaaagcgtga aaaacagaac
     2881 tctgggaagt gtttttatcg tggcgggaac cacaattggc gcaggcatgc tggcaatgcc
     2941 gctggctgcg gcaggtgttg gttttagcgt cacgttaatc ttgttgattg ggctttgggc
     3001 gttgatgtgc tacacggcgc tattactgct ggaggtgtac cagcatgttc cggcagatac
     3061 cggtcttggc acgctggcaa aacgctatct gggacgctac ggtcagtggc tgacgggctt
     3121 cagtatgatg ttcttaatgt atgctctgac tgcggcatac atcagcggtg ccggtgaatt
     3181 gttggcctcc agcatcagtg actggacagg tatttcaatg tcggcaaccg ctggcgtgct
     3241 gttgttcact tttgttgccg gtggcgtggt ttgtgtcggg acatcgctgg tcgatttgtt
     3301 taaccgtttt ctgttcagcg ccaaaattat ttttctggtg gtgatgctgg tattgctgct
     3361 accgcatatt cacaaagtga atcttttaac cctgccgttg caacagggac tggctctgtc
     3421 tgcaatcccg gtgattttta cgtcgtttgg ttttcacggt agcgtgccga gtattgtcag
     3481 ctatatggat ggcaacattc gtaagctacg ctgggtgttt ataatcggta gtgcgatccc
     3541 cctggtggca tatattttct ggcaggtggc gacgcttggc agcattgatt caacaacctt
     3601 tatgggattg ctggctaatc atgccggatt aaacgggctg ttacaggcat tacgcgaaat
     3661 ggtggcctct ccgcatgttg agctggcagt gcatttattt gctgatttag ccctcgccac
     3721 gtcatttctc ggcgttgcgt taggtttatt tgattatctg gcagatttgt ttcagcgttc
     3781 aaataccgtt ggtggacgat tgcaaactgg tgcaattacc tttctgccgc cgttggcgtt
     3841 tgcactgttt tatccacgag gatttgtgat ggcgctgggt tacgccggtg tggcgctggc
     3901 ggtactggca ttgattatcc cttcactatt gacctggcaa agcagaaagc acaatcctca
     3961 ggcgggttac cgggtcaaag gtggtcgtcc ggcgctggtg gtggtgtttc tctgtggtat
     4021 tgctgtgatt ggcgtgcaat ttttgattgc ggcagggttg ttaccagaag tggggtgatc
     4081 agatagcctc aaattcctta ttgggtgcca gaattaacgc tgacacccaa tttgacctct
     4141 taatgcaggc agcactgctt aaatttctta ccactgccgc acggacaagg atcgttacgc
     4201 cccggtttct cctctgcttt gatcggttgc tgaacagctt tttcctgcgg atgcgccatc
     4261 cagtatgcat gtaaatcaag cgccgccagt cgaatggcat ctacgctctc ttcaaacgct
     4321 tctggcgaca ttttctctac ccgctcgaag ttttcctcag taccgtgcag cgcaatcgcc
     4381 tccagcgctg gttttaacga atcgggcaac gttgaccagt cagaaagtgc cacgccccgc
     4441 atatagccaa agcaccactc ctcaacaatc gtcagctcgc tgccatcaac ttctcgcaag
     4501 ccgaataacg gctcaaactg ctccgggaat tcgttcagac gctctgcggt atcggccata
     4561 tgttgaaaag ccaggttcat aaagcgcgtc atctctttct ctgacgccca gcgcggcaca
     4621 tagtcagccc caccccacac ggcaaccagc cactgttccg gttcaatctc ttgcggagaa
     4681 ctcaacaccg ccgtcaataa accgtccagc tccgccacat caaggatggc gtggtcagtg
     4741 ttgtatttgg tcagaatatc gtccagccat tccaactcac tttcgtttaa cggtcccgtt
     4801 ttcatacgct tttccttgtg gatctcaact cgccagcacc tatcttacat gccggtccgt
     4861 atcagagata ctttttgagt ggctttgctg gtgattaaaa attaaggagg gtgtaacgac
     4921 aagttgcagg cacaaaaaaa ccacccgaag gtggtttcac gacactgctt attgctttga
     4981 ttttattctt atctttccca tggtacccgg agcgggactt gaacccgcac agcgcgaacg
     5041 ccgagggatt ttaaatccct tgtgtctacc gattccacca tccgggctcg ggaagaaagt
     5101 ggaggcgcgt tccggagtcg aaccggacta gacggatttg caatccgcta cataaccgct
     5161 ttgttaacgc gccaaattct tcaggccttt cagccagaca tccgcttgag gccgatgtct
     5221 tttaaactgg agcgggaaac gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc
     5281 tctaccaact gagctattcc cgcattcatc aagcaatcag ttaatcactt gattttatta
     5341 tcgtctggca atcagtgccg ccgttcgatg cgttgcattc tacttacctg gcgcgatgag
     5401 tcaacgatat ttttcaccac ttttgatcgt ttgctgaaaa ttgcgccgaa acgatcactg
     5461 atcaagcaaa tctgcacgcg cagcgctcaa atattgcaac attgaccaca gagtcagtac
     5521 cgcagccaca aagaaaagtg caataccggc gtactcaacc caaatgttcg gacgccacag
     5581 cagccatgcc aacgccacca tctgggcagt ggttttcact ttcccaatcc aggagacagc
     5641 cacgctactg cgtttaccca actccgccat ccattcgcgt agcgcagaaa taataatttc
     5701 acgggcgatc atcgttgccg ccggtaaggt cacccaccag ctgtgataat gctcggttac
     5761 cagcaccatg gcgatagcca cgagaacttt atctgcgaca gggtcaagga aagcaccaaa
     5821 ccgggtactc tggttccagc ggcgtgccag aaaaccatcg aaccagtcag tcaccgccgc
     5881 gacgcagaaa atgagcgcgg cggcaaacgg cgaccaggtg acaggcagat aaaagaccaa
     5941 tacaaagaat gggataagga tgacacggaa cagtgtaagc aacgtaggga tattaaattg
     6001 cataatgacg ggtaactatc tgttgtcagt aagattaccc ctatgttgct acagagacat
     6061 caatgtttca acgaccagaa gatcttttct gccagacctt gcgaaatacc cggcactttt
     6121 gcaatttcct cgacgctggc gttacgtaaa ccttgcaaac cgcccatata tttcaacaac
     6181 atttgccgac gttttggccc gacgccttca atggtttcca gggagctggt atttttgacc
     6241 ttcgcccgtt ttttacggtg cccgccaatc gcgtgatcgt gtgattcatc gcgaatatgc
     6301 tggataacat gcagcgcggg agaatctggc ggcaaactga atccctcacc ttccggctca
     6361 aagaacagtg tttccagccc agccttacga tctgctcctt tggcaacgcc aagtagcagt
     6421 ggatgatttt tatcccatga gacatccagt tcggcgaaga catttttcgc ctgcgcaagc
     6481 tggcctttgc cgccgtcgat aaggatcaca tccgggatct tactgtcgtc aatggcttta
     6541 ccataacgcc gacgcagcac ctgattcatc gccgcataat catcgcccgg cgtgatgcct
     6601 gtaatgttat agcgccgata ctccgcacgc agcgggccgt tagcatcaaa caccacacag
     6661 gaagcgacgg tttgttcgcc catggtatgg ctgatgtcaa agcactccat ccgcttcact
     6721 tccggcaatt tcaacacgct ggcaagcgcg gtcagtcgct ggtgaacggt agattgctgc
     6781 gaaagtttgc tggttaaggc cgtcgccgca ttggtgcgcg cgagtttcag ataacgcgcc
     6841 ctatcgccgc gaggtttggt ttgaacatta atcttgcgtc ccgccagttc tgaaagggaa
     6901 tcggcgagca gcgttttatc gctaagatta aaatcgagca ggatctcacc cggtaaggtg
     6961 cgcatctggc tgccttgtaa atagaactgg cctacgaagg tttctaccac ctcgctcagt
     7021 tccgtaccgc caggcacttt cgggaaatag ctgcggctgc cgagcacttt gccctgacga
     7081 atgaacaata cgtggacaca agccatgccc gcatcgaacg ccacaccaat aacgtcgagg
     7141 tcgtcgccgg tattggaaac aaactgtttt tcggtgacgc gtcgcaccgc ctgaatttgg
     7201 tcgcgaatac gtgccgcttc ttcaaactcc agattctggc tggcagtttc catacggcta
     7261 atcagttgcg taagcacctg atcatctttg ccagacaaaa acaggcgcac atactcgacc
     7321 tgctgagcgt attcttcttc actcaccagt ccttcaacgc acggtcccag acagcgccct
     7381 atctgatatt gcagacacgg acgcgagcga ttgcgataaa cactattttc gcactggcga
     7441 atggggaata tcttttgcag tagcgccagt gtttcacgta cggcatagcc attcgggaac
     7501 gggccgaaat attcaccttt ggcatgcttc gcaccacgat gcatcgccag acgcgggtgg
     7561 gtatcgccac tcaggaagat aaaaggataa gatttatcat cgcgtagcaa aacgttgtaa
     7621 cgcggctgat agagtttgat gtagttgtgt tccagcaaca gcgcttcggt ttctgtgtga
     7681 gtaaccgtta catcaatttg ctggatctgg gcgaccagcg cttcggtttt gcgcgaagcg
     7741 aggttgctac ggaaatagct ggaaagccgt tttttcaggt ctttcgcttt gccgacatag
     7801 ataaccgtac caccagcatc gtacatgcga taaacgcctg gctggctggt tacggttttt
     7861 aaaaacgctt ttgcgtcaaa ctgatcactc actgacttga taatgtctcc gcattacaca
     7921 gaccatggcg aattgccagg tgagtcagct caacatcgcc atgaatgttt agtttactga
     7981 acatacgata gcggtagctg ttcaccgttt tcggactgag attgagctgt tctgagatct
     8041 cattgacctt ctggcccttg gtgatcatca gcataatctg caattcacgt tcagacaaac
     8101 tggcaaatgg gctttctgtt ttttctggtt cgatctggct taacgccatt tgttgagcga
     8161 tgtcagaagc aatgtaacgc tgccctgaat agacagaacg aatcgcactc acgacttcct
     8221 gcggagccgc gcctttgctg aggtagcccg cagcaccggc ctgcatgact ttcgctggta
     8281 aagggttttc tgtatggacg gtaagcatga tgattttgac atcagctgtg gaacgcgcga
     8341 ttttacgcgt cgcctcaaga ccgccaatgc ccggcatact catgtccatt agcaccacgt
     8401 caacggcatt tgtccggcac cacttaacgg cgtcttcacc gcacgatgcc tcaccgacga
     8461 cttttatacc ctttatatct tccagaatgc gtcgtatccc tgcgcgcacc agttcgtggt
     8521 catcaacaag tagaacgttg atcaaaggaa tatctccaga aatagggata acgctactga
     8581 tagttagtca ttcgtatatt agcggttttt attgcaactt tgaaacgtta aaaatggtgg
     8641 gtattcgatt tttctctcgt ttttgtcaat tcgattgtcc acagttatgg tcacgcccgt
     8701 caccaacggt ttaccggatc gtttcacagt aagccctacg atgaaatgtg acaaaaattg
     8761 actttattca gcaaaaatga aaatcagccc gctgaatatg taacattaat taacccgaac
     8821 aatacgggca aaaaacattc atgataaaaa tatttattgg tcattatatt aacgcattta
     8881 atagcaccgt tgacgatacg ctcaaaaaac aaccactgct atttttagca aagcttatgg
     8941 tatactccgc cgccttaaca tttttcaccg caaattttca ttgcaacatg acgaggaaaa
     9001 taaatgagta cgcctgattt ttctactgcc gagaataatc aagaactggc aaatgaagtc
     9061 tcctgcctga aagcgatgct gacgctgatg ctgcaggcga tgggacaagc tgacgcgggc
     9121 cgcgtgatgt taaagatgga aaaacagctt gcgctgatcg aagacgaaac ccaggctgca
     9181 gtattttcca aaacggttaa gcaaattaaa caagcctacc gtcagtaata taaaaccggc
     9241 tgagagcgtg cctttcagcc ggtttttgca tctgacacgc agaaagaaaa gagatcaaat
     9301 taagccagta gcggccgcgt aacaggccac ctgggtctta tttggtgcat tgattttttt
     9361 ctgcatgttt ttctgatgga aattgaccgt attctcagag attgacaaaa tcatcgctat
     9421 ctctgctgat gtcttccctt ccgccgtcca tttcagaatt tctttttcgc gcttgctgaa
     9481 attcatctct ggcgtcatca ctatttcatc atttaaacgc atcagagcca tcagactttc
     9541 gcgcaccagt aactgcattt ttaattgcag ttcatcacta agaatgggta tttcgcgcgt
     9601 gctgcaacgg gaaaaggaca aaaagcccag cgcccgattt gacagcatta aatactgagt
     9661 gacaccgcgg cgtaaaccat gtgcgtgcgc ggcttcccat aatggctgtg cttcgctgaa
     9721 taagtcatca ttccacatta aatggccctg actaaagttt tcagggttca gcacaggatc
     9781 aattgcgaga aaattttttg cctgataata actaacccac gactcagggt aattggtgta
     9841 gaaagccact ttaggtcgcg tgaatggtac cgggtggcga acacataacg aatagtaatc
     9901 gtactccagc tgctgagcct gaagctctat ttcatggtag acctcttctg cggcctccat
     9961 cctctgaaaa cgcaacagca tcgtgcgacg ccagctgaaa aaatccgtat cctgcatagt
    10021 aaaccgcaac gcccctgaga gtgagtatca tttataatga taattcaaac tatcacataa
    10081 ataagattta tatacaattt atattattca ggcaatgaat tacttttgca agccatcgca
    10141 ttctcttatg ttattaatga gttatgctga tttgttaagc agttttatca cgcttgaaat
    10201 ggcgtcccgc cccgacaggt gaatcgtcgg ggctgatttt ttcttattat tgcagcagaa
    10261 acttctcgag gaactggcgg gtgcgaggct gctggggatc agcaaataac gcttttgctg
    10321 ccccctgctc gactatccgc ccctggtcca taaaaatcgc ccggtccgca acatcccggg
    10381 caaagctcat ttcgtgcgtc acaatcacca tcgtgcgctt ttcctgcgcc agctggcgga
    10441 ttgtgttcag gacttcgccc accagctctg gatccagcgc tgacgttggc tcgtcaaaca
    10501 aaatcacctc aggacgcatt gccagcgcac gcgcaatcgc aacacgctgc tgttgaccgc
    10561 cagacaaacg acgtggatag ctggtttctt taccggccag cccaactttc gccagcagct
    10621 cgcgagcgcg cgccgtggcc tcttctttcg gttcgccttt gacgatcacc ggcccttcaa
    10681 taatgttttc cagcaccgta cgatgcggaa acaaattaaa gttctggaag acaaacccga
    10741 cgtgctgacg caactggcga atcagcgatt tttgctgact taatgaacgt gcagtatcaa
    10801 tagtgatatc gccaacggtg atcgttcccg cttccggttg ttccagcaga tttatgctac
    10861 gtagcaatgt ggttttgccg gaaccactcg gaccgataat tgccaccact tcgccaggct
    10921 ttacctcgag gtcgataccg tgcagcaccg tctgaccgtg gaattttttc accaggttct
    10981 taacttcaat ggcactcatt ttggttctct ctcctggcga ttaagttgat tctcaaaatg
    11041 gttctgcaac gtcgataaca cagtcgccat gatccagtag atgagcgaag ccgccaggta
    11101 catggtgaac acctccagcg tgcgcgaggt aatcaactgc gcctgacgga acagctccgg
    11161 cacctggatt gtcgcggcca gcgacgtatc ttttaccaga ctgataaagc tgttcgacag
    11221 cggcggcaac gccacccgcg ccgcctgtgg caaaatcgcg cgacgcatgg tctgccacgg
    11281 cgtcatacca atactggcag cggcttccca ctgaccttta tcaatagaag aaatggcggc
    11341 gcgcagcgtt tcggcggcat aggcggcagt gttcagcgac agaccaatca ttgctgacgg
    11401 aatgggatct aattcgatac caaactgcgg caggccgtaa tagatcataa acagttgtgc
    11461 gatgagcggc gtaccacgaa aaatagagat ataaaaacgc gccagccagc gcacgggcca
    11521 gattggcgac aggcgcatca gcgcgagaat aaacccgagt agtaagccaa aaaacatgcc
    11581 gccgatactg agttgcaggg tatacccagc ccctttcaac aggaacggca aggaatcaat
    11641 aaccagttgt atactttctt gcatgagcgt tcccggagct aaacgtgggg atgataggcg
    11701 aacagcgcag gcgcgccgcc ggtatgaata aacagaatcg gcccttcatc tttgaagcgt
    11761 ttctgactga taccgtcaat cagccccgcc atcgcttttc cggtatacac aggatcaagc
    11821 agaatgcctt ccagacgcgc cagcaatttc actgcttcca tgccttcgtc gttcggcacg
    11881 ccgtagccag gtgcaaaata gtcatcccag agtaaaattt ccgctgatgc ggtcagctcc
    11941 agttctttcg caatcgcctg ttgtaggtta accactttcg gcaattgatc ggcaacggaa
    12001 cgcgacacgg taacgccaat cagttcgctt tcaggcataa ggtgttccag cccaacagcc
    12061 agtccggcgt gagttccggc actgcccgat gcgactacca ccgacgaaat attaaccgcc
    12121 ccttcacact gttgcgcgat ttccagcgca ctctccacat aacccagcgc gcccagagca
    12181 ttagaaccgc caaccggaat gacatacggg cgaaagcctt gtgcttcgac tcgcgtcgcc
    12241 agctcttcca gttgggcatt gggatcggtc agtgcgtcac acatttcaat ctgggtattg
    12301 aacagatcca gcaacaaacg attgccgttg gttaaatagt tttctgcggt tgtgccaata
    12361 ggattttcca gcagcgccac gcagtgcaga ccgagtttcg ccgcgactgc ggcagtctgg
    12421 cgcacatggt tagactggat cgcgccggca gtaatcagcg tatcggcacc ttcgcgcagg
    12481 gcatctgccg cgagaaattc cagcttacgt aatttattgc cgcccattgc catgggtgtg
    12541 acgtcatccc gtttgatgaa aatttcccgt ccaagataat cagaaaagcg cggcagatat
    12601 tcgagcggcg ttggcgcgcc gataaactcc agccgtggaa aacgggttaa attatgcagt
    12661 ggcataatag cctccgatgt gtgttgttgt gattttctta ttatgcacgc tgaaaacgcg
    12721 taaataaaaa aggcgctagt gaaagcgccc ttttttgtca ttatgctgat tatttggtca
    12781 catcagcacc aaaccatttt tcggaaaggg cttgcagagt gccatctttt tgcatttccg
    12841 caattgcatc gttcactgct ttcagcaggt cttcatttcc tttacgcagc gccacgccag
    12901 actcctgacg ggagaatgct tcaccggtta ctgccagcgt atcgttggtt ttcttcacca
    12961 gatccagcgc cgccagacgg tcaacgagga tcgcatcgat acggcctacg cgcagatcct
    13021 gatatttggt cgggtcatca tcataggtac gcacatcgac gccctgaaca ttctgccgca
    13081 gccactcttc atagttggtg cccagaccga cacccacttt tttgcctttc agatcagcag
    13141 ccgttttaat ggtgccttcg ttaccttttt tcaccagcgc ctgaatacca gaaatggtgt
    13201 acggggtaga gaaatcgtat tttttcttgc gctcatcaga aatggtgacc tgattaatca
    13261 ccacatcaat gcgtttagag tccagcgacg ccagcatacc gtcccatttg gtcggtttta
    13321 gtgacgcctc aacgccaaga tgttttgcca gctgttgggc aaattccact tcaaaaccgg
    13381 ttaatttgcc gtcatccccc tgaaaactga acggcggata agttccttcc agccctacca
    13441 gtagcgtacc gcgctctttg actttattaa gcagaccttc atctgcaaaa cttttaacgc
    13501 tcatgcccgc aaccagcgcc acggccatca cacccatcaa tgcctgacgt cccagatgtg
    13561 ctaatttcat attcaccccg aatgttgtta tgtctgtttg cagtgtagag ccattgtttg
    13621 taaacacaaa aacaactccg ctacatctta ttcttattta atatatatca gaagcaggca
    13681 ggctggagga tttctgcaca agtccgctac acgtttgacg ctgatagtgt tgatacttcc
    13741 gtaatgcgat gcggtaattg ttggtgctgg tggtgggcag ccacggggcg aggatttcat
    13801 caaggaaacc gtcctgcagc ggatcgaggg aaatattttg ctcgtgtaga tgattcccca
    13861 aacggcgcaa acgaacgaca tattcacgca ccgtacctgg gctcatttca gtttgctcaa
    13921 aaagaaattg cttaaagccg ataatatcga aaaaatcact ctgcgttttg caatgtaaat
    13981 caccgcaaaa tcggcacaat gccgcccatg atttttgttc cgtttgccag ccattttcat
    14041 caagcagcgc gtccaggcgg gaaatctcga ttttattcac tattttgccg tcgcgaacga
    14101 gggtaatgcg atcgagtaat ttacggcaat gcgcgcaatg agtctggctg tgtttaaaat
    14161 ctttaagata gcggcttaat ggccgtcttt tcaggtgctg caccatcatt aagaactcct
    14221 ggtagtcaaa attaaagtgc ggcatttact gacgttataa cttacccagt ttagtgcgta
    14281 accgtttaat ggcctggctg tgtaactgac tgacccgcga ttccccgacc tccagcaccg
    14341 caccaatctc tttgagattc agctcttcct ggtaatagag ggttaatacc agtttttcgc
    14401 gctccggcaa cgtttcgata gcttccatca cccgctggcg cagattactg tccagcagtt
    14461 gttgtagcgg gttttctcgc tgatgatcat cagtaaccag ttcgatgcta tcgccgtgct
    14521 cttcgcgcca ctcatcgtag gagaagagct ggctgttatt ggtgtcgagc aacatttggc
    14581 gataatcggc aatatcgatc cctaaacgtt ccgctacctc agtttccgtg gcgttgcggc
    14641 caagttcctg ctccagttgc cctattgcct gtgccacttc acgcgcgttg cgtcgcacgc
    14701 tgcgcggcac ccagtcacgg ctgcgaagtt catccagcat agcgccacgg atacgctgca
    14761 ctgcgtaagt tgtaaatgcc gttccttgta gggcgtcata gcgttcgacg gcattaagta
    14821 acccaatgcc gcccgcctgt agcagatcgt caagttccac gctcgcgggc agtcgaacct
    14881 gcaggcgcaa tgcttcgtga cgcaccagcg ggacataacg ctgccacagc gagtgtttat
    14941 ccattacacc ttcagcggta tagagtgaat tcacgataaa cagccctgcg ttatatgagt
    15001 tatcggcatg attatccgtt tctgcagggt ttttaatcgg acgattagtg ggtgaaatga
    15061 ggggttattt gggggttacc ggtaaattgc ggagagaaaa acccccgccg gtggcggggg
    15121 agcacgttgc tgacaaattg cgcttcatgt tgccggatgc ggcgtaaacg ccttaaccgg
    15181 cctacaaaaa tgtgcaaatt caataaattg caattcacct tgtaggcctg ataagcgcag
    15241 cgcatcaggc aatttggcgt tgccgtcagt ctcagttaat caggttacga cgattaaccc
    15301 tgcagcagag acagaacctg ctgcggtacc tggttagctt tggacagcac ggagttacct
    15361 gcctgctgga tgatctgcgc tttcgacatg ttggacactt cggtcgcata gtcggcgtcc
    15421 tgaatgcggg actgcgcttc agacaggttg gtggtggtgt tgttcaggtt ggtgatagcg
    15481 gaatcgagac ggttctgcac ggcacctaga gaagaccgga atttgtcgac tgatgcaata
    15541 gctttatcca gaagcgccag cggatcctca gttgaagcac cttcaaattg ctcagtcgca
    15601 gttgtggtta ccttaccatt agtatcagta acatcactta aattcagatc agttaactca
    15661 agagctgttg ctccattctt aagaatgtct gccgcatcac cagcagaata gtttttgcct
    15721 tgaagtgtga cgaaaccttg agctgtgccg tctttatctg ctgaaacttt gatcagctga
    15781 cctgtttgct tggcatcgtc aacaccgttc gcataatcat cataagtaac atcaatggtg
    15841 tttaaggtta ctttacctga agcggcatta acagaagcag catagaagtc tttacctgac
    15901 tgaacaacat aatcagctgt tgccttacca tctttatcaa ggatgttgtg cagcgacagg
    15961 cttgacgtat cggaaacccc caatttggct gcgattgctg cagactcatc agtatcaccg
    16021 aagttaacat caacaggttc attgccagcg gcttgcggga tagatgtgat agcattgctg
    16081 acattaagag agtttttaga aactgagaag ccattcagcc ccaaagttga agaatcaatt
    16141 ttcttcagat cgatggagat agtttgacca tcattcgcac caacctgaat tttcattgaa
    16201 ccatctttgg aaagaacatt aacgccgttg aactgagtct gaccggatac gcggtcaatt
    16261 tcatcgagac gggatttgat ttcgtcctgt attgaagaca ggtcagaatc agagttagta
    16321 ccggtagtgg cctgaacggt caattcacga acacgctgca ggttgttgtt gatttcagac
    16381 agtgcgcctt cagtggtctg cgccagagag ataccgtcgt tggcgttacg tgcggcctga
    16441 gtcagacctt tgatgttaga agtgaagcgg ttagcaatcg cctggcccgc agcgtcatct
    16501 ttagcgctgt taatgcgcag accagaagag aggcgctcga tagaagtcga cagcgcagac
    16561 tggtttttgt tgatgttgtt ctgagtgatc agcgagaggc tgttggtatt aatgacttgt
    16621 gccatgattc attttcctgt tttcaagtct acggtttaag tattgggctt ccacccttcg
    16681 gcttcgtcgc cgtcaaccct gttatcgtct ggtcactcac aacctttaga atttttttct
    16741 agcggtcgaa atactcacct tcctcgttgc tgttcgtgtc attaagccaa aaaataaatc
    16801 gtaaactttg ccccgcttac gccgataacc ccggtattcg ttctatacgt cgcatgatta
    16861 aggaatttat atggctagta tttcaacgct tggggttggg tcggggctgg acttaagcag
    16921 catccttgac agtctggagg cggcagaaaa atctaccctg acgccgatat caaaacagca
    16981 aagctcttac accgcgaaac tgagcgccta cgggacgctg aaaagtgcac tagagagttt
    17041 ccagacagcc aacacagcac tgaataaagc cgatttattt accgccactt caaccaccag
    17101 cagttcatcg gctttcagcg ccaccaccac gggcagcgcg attgccggga aatacaccat
    17161 tagcgttagc caactggcgc aggcacaaac gctgaccaca aaaattagtc aaaaagacag
    17221 taaagctgcc atcgccacca gcgatagcgt actgactatc cagcaaggcg gcggtaaaga
    17281 tccggtcacg attgatatca gcgcggctaa ctcgtcactt tccggcattc gcgatgccat
    17341 taacaacgcc aaagctggtg tcagcgccag tatcatcaat gtgggaaacg gcgaatatcg
    17401 tctgtccatc accgctaacg atacgggcaa cgataacgct atgaaactta gcgtcagcgg
    17461 cgacagcgcc ctggaaagtt ttatgggtta caacggaacg tcgggtgata gcagcaatgg
    17521 catgattgaa agcgtcaccg cgcaaaacgc caaactgaca gtcaataacg ttgagattga
    17581 aaacagcagt aacactatca gcgacgcgct ggaagatatc acactcaacc tgaatgacgt
    17641 caccaccggt aatcagacac tgaccatcag taaagacaca tccaaagctg aaaatgcagt
    17701 caaagcatgg gtggatgcct ataacacatt gcaggataca ttcagtagct taaccaaata
    17761 caccgctgtc gatgccggag cagaaagtca ggactccagt aatggagcat tacttggtga
    17821 ctccacactg cgtaccatcc agactcagct taaaaccttg ctcgccaata ctcacagcag
    17881 ttcaaactat aaaacgctgg cacagattgg cattaccagc gatgccagta ccggcaagct
    17941 ggaaatcgcg accgacaaac tgcaaacagc actaaaaaaa gatgccgctg gcatcggtga
    18001 aatgtttata ggtgacggta aaagtacagg ggtaactacc ggcattagta gcaacctgac
    18061 cagttggctc tcttccaccg ggataatcca ggccgcaaaa gatggcgtca gcaaaacgct
    18121 gaataatttg accgaccagt acaacgcagc cagtgagcgt atcgatacct taatgacacg
    18181 ctataaagcc cagttcaccc aattagatgt gttaatgaac tcgcttaact ccaccagtag
    18241 ctatctgaca cagcagttcg acacctcaaa tagcaactcg aaataatctt caggagaaac
    18301 tatgtacgcg gcaaaaggca cccaggccta tgcacaaatt ggcgtcgaaa gcgccgtaat
    18361 gagcgccagc cagcagcagc tggtcaccat gctatttgat ggagtgctga gcgcactggt
    18421 tagagcgcgc ctgtttatgc aggacaacaa tcagcaaggc aaaggcgtct ctttgtcaaa
    18481 agcgatcaac atcattgaga acggactgcg ggtgagtctt gatgaaaaga gcaaagacga
    18541 attaacccaa aacttgattg ctctttatag ctatatggtc aggcgcttgc tgcaagccaa
    18601 tttacgcaac gatgtctccg cagtcgaaga agtggaagca ttaatgcgta atattgccga
    18661 tgcctggaaa gagtcgttac tctccccttc tttgattcag gacccagtct gatgaaccat
    18721 gcaccgcatt tgtatttcgc ctggcaacaa ctcgtcgaaa aaagccagct catgttacgc
    18781 ctggcaacgg aagaacaatg ggacgaactc atcgccagcg aaatggcgta tgtgaatgcg
    18841 gtgcaggaga ttgcacattt gactgaagag gttgacccgt ccaccacgat gcaggagcag
    18901 atccgcccga tgctgcgcct gattctcgac aacgaaagca aggtaaagca gttattacag
    18961 attcggatgg atgaactggc gaaactggtc ggtcagtcat cggtgcaaaa atcggtgtta
    19021 agtgcctatg gcgatcaggg cggctttgtg ctggctccgc aggataacct cttttgaatc
    19081 tgaacgagtc gatggctcgc gaataatctg attacggcta cgcttctaat gttccccttg
    19141 aatggagtcg aagaatgcgt aatcccacgc tgttacaatg ttttcactgg tattacccgg
    19201 aaggcggtaa gctctggcct gaactggccg agcgcgccga cggttttaat gatattggta
    19261 tcaatatggt ctggttgccg cccgcctata aaggcgcatc gggcgggtat tcggtcggct
    19321 acgactccta tgatttattt gatttaggcg agtttgatca gaaaggcagc atccctacta
    19381 aatatggcga taaagtacaa ttgctggccg ccattgacgc cctgaaacgt aatgacattg
    19441 cggtgctgtt ggacgtggta gtcaaccaca aaatgggcgc ggatgaaaaa gaagctattc
    19501 gcgtgcagcg tgtaaatgct gacgaccgta cgcaaattga tgaagaaatc attgagtgcg
    19561 aaggctggac gcgttacacc ttccccgccc gtgccgggca atactcacag tttatctggg
    19621 atttcaaatg ttttagcggt atcgaccata tcgaaaaccc tgatgaagat ggcatattta
    19681 aaattgttaa cgactacacc ggcgaaggct ggaacgatca ggttgatgat gaattaggta
    19741 atttcgatta tctgatgggc gagaatatcg attttcgcaa tcatgccgtg acggaagaga
    19801 ttaaatactg ggcgcgctgg gtgatggaac aaacgcaatg cgacggtttt cgtcttgatg
    19861 cggtcaaaca tattccggcg tggttttata aagagtggat cgaacacgta caggaagttg
    19921 cgccaaagcc gctgtttatt gtggcggagt actggtcgca tgaagtcgat aaactgcaaa
    19981 cgtatattga tcaggtggaa ggcaaaacca tgctgttcga tgcgccgctg cagatgaaat
    20041 tccatgaagc atcgcgcatg gggcgtgact acgacatgac gcagattttc acgggtacat
    20101 tagtggaagc cgatcctttc cacgccgtga cgctcgttgc caatcacgac acccaaccgt
    20161 tgcaagccct cgaagcgccg gtcgaaccgt ggtttaaacc gctggcatat gccttaattt
    20221 tgttgcggga aaatggcgtt ccttcggtat tctatccgga cctctacggt gcgcattacg
    20281 aagatgtcgg cggtgacggg caaacctacc cgatagatat gccaataatc gaacagcttg
    20341 atgagttaat tctcgcccgt cagcgtttcg cccacggtgt acagacatta tttttcgacc
    20401 atccgaactg cattgccttt agccgcagtg gcactgacga atatcccggc tgcgtggtgg
    20461 tcatgtcgaa cggggatgat ggcgaaaaaa ccattaatct gggagagaat tacggcaata
    20521 aaacctggcg tgattttctg gggaaccggc aagagagcgt agtaaccgac gaaaacggtg
    20581 aagcgacctt cttttgcaac ggcggcagcg tcagcgtgtg ggttatcgaa gaggtgattt
    20641 aaattcatcc ccggcggcaa gccggggaga tttcattacg gcagtggcgt cggtaacggc
    20701 gctttatcca gcgcggcggc acactctgcc gtcgggcgat ccacacgctc catcgtcata
    20761 ccatcgtact caatggtgtt accgtcacgc tcaacctcgt acagctcacg ttttacggta
    20821 acgttagtga gatcatcaga catcaatgtc aacttgcccg gcacggcaat cacccgctgc
    20881 cactgacggc aatccagggt atcgccctct ttagtgacaa tcaggctgcc tatcgcttcc
    20941 ggactgacca gcgcacgttg cggccctttg gtctgccagt agcccgccag ccagtccggc
    21001 gcgggggttt tcacgacatt gttatagttt tcgacttcgg cacagcccgc cagcaacatg
    21061 agcgcgcctg caattgcgag ttttttcatc attcgtcctg catgagaaga aaagaggatt
    21121 gtggcattaa agccctgatg tcgccagcct ttccaggttc agagagaaat ttgatctaac
    21181 tattattttt gtgaattatt accccgaaaa taaatttgca tgatgtatga ttcgcgattc
    21241 ttgtttcaaa ccttctgagt tcagaggcta cattcatgtc atggcagcaa ttcaaacacg
    21301 cctggttgat taaattctgg gcacccatcc ctgcggtcat cgcggcgggt attctctcca
    21361 cttactattt tggcattact ggcacctttt gggctgtcac gggtgaattt acccgttggg
    21421 gcggtcagct cctgcagctg tttggcgtcc atgctgaaga gtggggttat tttaaaatta
    21481 tccatctgga aggatcgcca ttaacccgca tcgacgggat gatgatcctc ggcatgtttg
    21541 gcggctgctt tgccgcagca ctgtgggcca acaatgtcaa actacgcatg ccgcgcagcc
    21601 gtatccgcat tatgcaggcc attattggcg gcattatcgc cggttttggc gcgcgtctgg
    21661 caatgggctg taacctggcg gcgttcttta ccggcattcc tcagttctcg ctgcatgcct
    21721 ggttctttgc catcgccact gccattggtt catggtttgg cgcgcgcttt accctgctgc
    21781 ccatcttccg tattcccgtg aaaatgcaga aagtttctgc cgcctcaccg ctgacgcaaa
    21841 aaccggatca ggcgctgcgt cgttttcgtc tcgggatgct ggtctttttc ggcatgctgg
    21901 gctgggcgct gctcacggcg atgaaccaac ccaaactggg gctggcaatg ctgtttggcg
    21961 tcggctttgg tttactgatt gaacgtgcgc aaatctgctt tacttcggcg ttccgcgata
    22021 tgtggatcac cggacgtacc catatggcga aagcaatcat tatcggtatg gcggtgagtg
    22081 ccatcgggat cttcagttac gtacagttag gcgttgaacc caaaatcatg tgggcgggac
    22141 caaacgcggt aattggtggt ttactgtttg gttttggcat cgtgctggct ggcggctgcg
    22201 aaaccggctg gatgtaccgc gcggtagaag gccaggtgca ctactggtgg gtcggtctgg
    22261 gcaatgtgat aggctcaacg attctggcgt attactggga tgatttcgct ccggcgctgg
    22321 ccaccgactg ggacaaaatc aacctgctga aaacctttgg tccgatgggt ggcctgctgg
    22381 tgacatattt gctgttgttt gctgcgctga tgttgattat cggctgggaa aaacgcttct
    22441 tccgccgtgc ggcaccgcag actgctaagg agatcgcatg aaaaatatcg ttcctgatta
    22501 ccgccttgat atggtgggtg aaccctgccc ttatccggca gtcgcaactc ttgaggcgat
    22561 gccgcagtta aaaaaagggg aaatcctgga agtggtgagc gactgtccgc agtcgatcaa
    22621 taatatacca ctggatgcgc gtaatcacgg ctatacggtg ctggatattc agcaagacgg
    22681 gccgaccatt cgttatttaa ttcagaagta atccctcatt cccgctgtgg cccacagcgg
    22741 gaacatcgtt acctttgtat cacatagatt ttacccgccc cctctcctat tcttttcagt
    22801 atgatctcag gaggtttcta tgtgtggacg ctttgcccaa tcccaaacgc gtgtagatta
    22861 ccttgcgctt ctcgcggaag atattgaacg cgatattccc tatgatcccg aacccattgg
    22921 cagatacaac gtcgcgccgg gaaccaaagt ccttctgctc agtgaacgtg acgaacacct
    22981 tcatctggat ccggttttct ggggatatgc tcccggatgg tgggataaac cgccactgat
    23041 taacgcccgc gtagaaactg ccgcaaccag tcgcatgttt aaaccgctct ggcaacatgg
    23101 tcgggcaatc tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaca
    23161 gccttatttt atctatcgcg ctgatggaca acctgttttt atggccgcga taggtagcac
    23221 accgtttgag cgcggtgacg aagccgaagg atttttaatt gtcactgcag cagcagacca
    23281 gggtctggta gatattcatg accgccgccc gctggttctg tcgccagaag ctgcacagga
    23341 atggatgcgg caagagattg gcggtaaaga agcatcagaa attgctacca acggctgtgt
    23401 gccagcaaac cagttcacct ggcacccggt atcgcgcgcg gtaggtaatg tcaaaaacca
    23461 gggcgcggag ttaattcaac ctgtttgttg acatcctcca cgccctgaag gacgaggatc
    23521 cctgctacgt tcaggctgtc gcctgaatca tttcggtggg ttcctgcttc aacgggcggc
    23581 ctgactgcac catctctcca caggcaagaa cggcgtgccc tgcagctaaa atgttacgag
    23641 cgccatttac atcggcgttc gctgtatatc cacatacctg gcatctgaat ttactttgtg
    23701 acaggcggtt ttctttcgct gtatgaccac agcacgcgca acgctggctt gt
//
LOCUS       DAZKIV010000066        23238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZKIV010000066 DAZKIV010000000
VERSION     DAZKIV010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.066"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1101
                     /locus_tag="ACHOT6_002521"
     CDS             <1..1101
                     /locus_tag="ACHOT6_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9947717.1"
                     /translation="LAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVSIFYIFRQLS
                     FICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYNADYSLYVVD
                     YCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLASLCCNLLLL
                     TLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLTNIYNRRYFF
                     NSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQQSIRPDDIL
                     ARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTISIGTVFSTG
                     DTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1282..2772
                     /gene="dgcJ"
                     /locus_tag="ACHOT6_002522"
     CDS             1282..2772
                     /gene="dgcJ"
                     /locus_tag="ACHOT6_002522"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFP9947718.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNRSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQAGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            2815..3318
                     /gene="yeaK"
                     /locus_tag="ACHOT6_002523"
     CDS             2815..3318
                     /gene="yeaK"
                     /locus_tag="ACHOT6_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFP9947719.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHKAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(3319..3423)
                     /gene="yoaI"
                     /locus_tag="ACHOT6_002524"
     CDS             complement(3319..3423)
                     /gene="yoaI"
                     /locus_tag="ACHOT6_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFP9947720.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3593..4039
                     /gene="yeaL"
                     /locus_tag="ACHOT6_002525"
     CDS             3593..4039
                     /gene="yeaL"
                     /locus_tag="ACHOT6_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFP9947721.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(3996..4817)
                     /gene="nimR"
                     /locus_tag="ACHOT6_002526"
     CDS             complement(3996..4817)
                     /gene="nimR"
                     /locus_tag="ACHOT6_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFP9947722.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSGRNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            4914..6095
                     /gene="nimT"
                     /locus_tag="ACHOT6_002527"
     CDS             4914..6095
                     /gene="nimT"
                     /locus_tag="ACHOT6_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFP9947723.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISHGYSKAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6150..6497
                     /gene="yeaO"
                     /locus_tag="ACHOT6_002528"
     CDS             6150..6497
                     /gene="yeaO"
                     /locus_tag="ACHOT6_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFP9947724.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTSQ
                     NHALVLADWLRSL"
     gene            complement(6519..6773)
                     /gene="yoaF"
                     /locus_tag="ACHOT6_002529"
     CDS             complement(6519..6773)
                     /gene="yoaF"
                     /locus_tag="ACHOT6_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFP9947725.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            6956..7981
                     /gene="dgcP"
                     /locus_tag="ACHOT6_002530"
     CDS             6956..7981
                     /gene="dgcP"
                     /locus_tag="ACHOT6_002530"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFP9947726.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8014..8112
                     /gene="yoaK"
                     /locus_tag="ACHOT6_002531"
     CDS             8014..8112
                     /gene="yoaK"
                     /locus_tag="ACHOT6_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFP9947727.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8115..8189
                     /gene="yoaJ"
                     /locus_tag="ACHOT6_002532"
     CDS             8115..8189
                     /gene="yoaJ"
                     /locus_tag="ACHOT6_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFP9947728.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8248..8496)
                     /gene="yeaQ"
                     /locus_tag="ACHOT6_002533"
     CDS             complement(8248..8496)
                     /gene="yeaQ"
                     /locus_tag="ACHOT6_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFP9947729.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(8644..8826)
                     /gene="yoaG"
                     /locus_tag="ACHOT6_002534"
     CDS             complement(8644..8826)
                     /gene="yoaG"
                     /locus_tag="ACHOT6_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFP9947730.1"
                     /translation="MGKATYTVTVTNNSNGVSIDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(8830..9189)
                     /gene="yeaR"
                     /locus_tag="ACHOT6_002535"
     CDS             complement(8830..9189)
                     /gene="yeaR"
                     /locus_tag="ACHOT6_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFP9947731.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(9362..10000)
                     /gene="leuE"
                     /locus_tag="ACHOT6_002536"
     CDS             complement(9362..10000)
                     /gene="leuE"
                     /locus_tag="ACHOT6_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFP9947732.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(10127..11050)
                     /gene="dmlR"
                     /locus_tag="ACHOT6_002537"
     CDS             complement(10127..11050)
                     /gene="dmlR"
                     /locus_tag="ACHOT6_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFP9947733.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            11153..12238
                     /gene="dmlA"
                     /locus_tag="ACHOT6_002538"
     CDS             11153..12238
                     /gene="dmlA"
                     /locus_tag="ACHOT6_002538"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFP9947734.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            12489..14099
                     /gene="yeaV"
                     /locus_tag="ACHOT6_002539"
                     /pseudo
     CDS             12489..14099
                     /gene="yeaV"
                     /locus_tag="ACHOT6_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
     gene            14131..15255
                     /gene="yeaW"
                     /locus_tag="ACHOT6_002540"
     CDS             14131..15255
                     /gene="yeaW"
                     /locus_tag="ACHOT6_002540"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFP9947735.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            15311..16276
                     /gene="yeaX"
                     /locus_tag="ACHOT6_002541"
     CDS             15311..16276
                     /gene="yeaX"
                     /locus_tag="ACHOT6_002541"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFP9947736.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(16330..17445)
                     /gene="rnd"
                     /locus_tag="ACHOT6_002542"
     CDS             complement(16330..17445)
                     /gene="rnd"
                     /locus_tag="ACHOT6_002542"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFP9947737.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(17527..19212)
                     /gene="fadD"
                     /locus_tag="ACHOT6_002543"
     CDS             complement(17527..19212)
                     /gene="fadD"
                     /locus_tag="ACHOT6_002543"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFP9947738.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(19417..19998)
                     /gene="yeaY"
                     /locus_tag="ACHOT6_002544"
     CDS             complement(19417..19998)
                     /gene="yeaY"
                     /locus_tag="ACHOT6_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFP9947739.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKNRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(20038..20733)
                     /gene="tsaB"
                     /locus_tag="ACHOT6_002545"
     CDS             complement(20038..20733)
                     /gene="tsaB"
                     /locus_tag="ACHOT6_002545"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFP9947740.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(20791..22701)
                     /gene="yoaA"
                     /locus_tag="ACHOT6_002546"
     CDS             complement(20791..22701)
                     /gene="yoaA"
                     /locus_tag="ACHOT6_002546"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFP9947741.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGGGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            22833..23177
                     /gene="yoaB"
                     /locus_tag="ACHOT6_002547"
     CDS             22833..23177
                     /gene="yoaB"
                     /locus_tag="ACHOT6_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFP9947742.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5689 a         5930 c         5639 g         5980 t
ORIGIN      
        1 cttgcatttt taagtggcct tatctattta gttgaaacca ttgtaattat ccataaacca
       61 attaacggca gtacactgat ccagacaaag tcgaatgatg tttctatttt ctatattttc
      121 cgccaactca gttttatttg tttaacctcg ctggcactct tttgttatgg aaaagacaac
      181 atccttgaca acaataagaa aaaaacggga atcctgttgc tggcgctgat tcctttttta
      241 gtttttcccc ttctggcaca caatctgagc agttataacg ctgactattc tttgtatgtc
      301 gtcgactact gtccggacaa ccatactgcg acctggggaa tcaactatac aaaaatattg
      361 gtttgtctgt gggcattttt actgttcttt attatcatgc gtacacgatt agccagcgaa
      421 ctatggccgt taatagcatt attatgtctg gcatcgctat gctgcaactt acttctactg
      481 actctggatg agtataatta caccatctgg tatatcagtc gcgggattga agtttccagt
      541 aaactgtttg ttgtgtcttt tctgatttat aacatttttc aggaactgca actctccagc
      601 aaactggcag ttcatgatgt gctgaccaat atttataatc ggcgctactt tttcaacagc
      661 gtagagtcat tattgtcgcg acctgttgtt aaggacttct gtgtcatgct ggttgatatt
      721 aatcagttca aacgcatcaa tgcccaatgg ggacatcgtg tgggtgataa agtgctggtt
      781 tcaattgtcg atattatcca gcaaagcatc cgccctgatg atattttagc gcgactggag
      841 ggtgaggtgt ttggcttgct atttaccgaa ctcaatagtg cccaggcaaa aatcattgcg
      901 gaacgtatgc gtaaaaatgt cgaactcctg accggcttta gtaacagata tgatgttcct
      961 gaacaaatga ccatcagtat tggcacggtt ttttcaacgg gtgacacgcg taatatctcg
     1021 cttgtcatga cggaagcaga taaagcctta cgcgaagcga aaagcgaggg gggcaacaaa
     1081 gtgattattc atcatattta agtgcaaaaa tattcagagc catgcttttt gcgtggcttt
     1141 tgcatacaat ttattactat tacccctaaa tttcccctcc ccagcggcgc agagatgagt
     1201 ataattagcg cccctgtgcc aggccgcaat cgaactttat ctggttttct cgtttcacta
     1261 accgaaggag tgccatttat catgaaattg caccatagaa tgctccggca ttttatcgcc
     1321 gcaagtgtca ttgtgctgac atcttccttc cttatttttg aacttgtcgc cagcgacaga
     1381 gcaatgagtg cctatctgcg ctatatcgtg cagaaagcag actcctcctt tctttatgat
     1441 aagtatcaga atcagagtat tgccgcgcat gtgatgcgcg ctctcgctgc tgagcagtcg
     1501 gaagtgtcgc cagaacagcg gcgcgccatc tgcgaggctt ttgagtctgc caataatacc
     1561 catggcttaa acctgactgc ccataaatac ccgggcttac gcggcacact acaaaccgca
     1621 tccactgact gcgacacaat tgtggaagct gcagcactat tacccgcttt tgatcaggca
     1681 gtggaaggca accgccacca ggatgattac ggttcaggtc ttgggatggc cgaagagaaa
     1741 tttcactatt atctcgatct caatgaccgc tatgtctatt tttatgagcc ggttaatgtt
     1801 gaatactttg cgatgaataa caggtccttc ctgcagtcag gaagtattgg catcgatcgc
     1861 aaagatattg aaaaggtatt taccgggcgt accgtattgt cgagcattta ccaggatcag
     1921 cgtactaaac agaacgtgat gagtttgctg acgccggtat atgtcgcagg gcagctaaaa
     1981 gggattgtgc tgctggatat taacaaaaac aatttgcgga atatctttta cactcatgac
     2041 cgccctctcc tctggcgttt tctcaatgtc acgctaaccg ataccgattc ggggcgcgac
     2101 attatcatca accagagcga agataatctg ttccagtatg tcagttacgt ccatgactta
     2161 ccgggcggca ttcgtgtctc gttatccatt gatattcttt actttatcac gtcttcgtgg
     2221 aaaagcgttc tgttctggat tttgacggcg ttaattttgc tgaatatggt ccggatgcac
     2281 ttccgtttat accaaaatgt gtcgcgagaa aatattagtg atgcgatgac tggactgtat
     2341 aatcgcaaaa ttttaacccc tgaactggag cagcggttgc agaaactggt gcaagccggt
     2401 tcttcggtga tgtttattgc aattgacatg gacaagttaa agcaaataaa tgacaccctc
     2461 ggtcatcagg agggggattt agcgattacg ttattagctc aggcgattaa acaatcgatt
     2521 cgtaaaagtg attatgccat ccgactcggt ggcgatgaat tctgcatcat tcttgtcgat
     2581 tcgacgccgc aaattgcagc acaactgcct gaacgtatcg aaaaacgtct gcaacatatc
     2641 gcgccgcaga aagagattgg cttctcttcc ggtatttacg cgatgaaaga aaacgatacg
     2701 ttacatgatg cgtataaagc ttccgatgag cgtttatatg tcaataagca gaacaaaaac
     2761 agccgttcat gataaccttc tgtggttgtt tgcttgtaat ctcaggagcg tgaaatgact
     2821 gaaatggcta aaggaagcgt gacgcatcag cggttaatcg ctttattatc acaagaaggt
     2881 gctaacttcc gcgtcgttac ccataaagcg gtaggtaaat gtgaagcggt gtcagaaatt
     2941 cgtggcaccg ctttaggcca gggagctaag gcactggtat gtaaagtcaa aggcaatggc
     3001 gtaaatcaac atgttctggc gatcctcgct gccgatcaac aggccgatct gagccaactc
     3061 gccagccata taggcggatt acgcgcttcg ttggccagcc cggcggaagt tgatgaactc
     3121 acaggctgtg tcttcggcgc gatcccccct ttcagcttcc atccaaaact caaactggtt
     3181 gccgaccccc tactcttcga acgatttgat gaaatcgcct tcaatgcagg catgctggat
     3241 aaatccgtta ttttgaaaac cgcggattat ttgcgcattg cgcaaccaga actggtcaat
     3301 ttccgccgca ctgcgtaact agcctgtccg ttcaataagc agaacggaca aaaccagcac
     3361 aacagcaatg gcaaaaaacg atgacgtgat aatcagtgtc tcgacgaaca tttgatcgtt
     3421 catagcatgc ccccgggata tagctgtttt cacgttattg ttagcgtgca caaatggcag
     3481 tttgatgaca gttcgcctga ttttttatgc aaaaaacgta aagattttct acttccttcc
     3541 tgcagcaagc gtaaagtaag caggcttatt attttttggc aaggaaacca cgatgtttga
     3601 tgtcactttg ctgatcctgc tcgggttagc tgcgctgggc tttatcagtc ataacaccac
     3661 tgtcgccgtt tcaattctgg tgttaatcat tgtccgcgtc acaccgttaa gcactttttt
     3721 cccgtggatt gaaaaacagg ggctaagtat cgggataatc attctgacga ttggcgttat
     3781 ggcccccatc gccagcggga cgctaccgcc ctcaaccctg attcactcgt ttctgaactg
     3841 gaaatcactg gtggcaattg cagtaggcgt tattgtctcc tggctgggtg ggcgcggcgt
     3901 gacgttaatg ggcagccagc cgcaactggt cgccgggctg ctggtaggca ctgttttagg
     3961 cgtggcgctg tttcgcggcg taccggttgg cccgcttatt gccgcgggtc tggtttcgct
     4021 gattgtgggg aaacagtagt taatcccgca atatatcggc ctggcgtttg ccccaggcct
     4081 ttcttaaaca tagtaataaa ggccgttgtt gaatcatacc ccagcgtatg agcaactttc
     4141 tgtacagtat cgcctttcac taatccctga agcgccataa tcagctgtaa ctgctgacgc
     4201 cactggcgaa aactcaaccc cgtctctttt actatcagtc gggcaagatt gcgtccactc
     4261 atcgcaaaaa agccagccca ttgccccaat gacccccact cgacgggccc tttcgccatc
     4321 atctccacca tcgtgcggat tttaggatga gaagaaacgg gcaggtgcag ctgttgttgt
     4381 ggctgctgag ggagttcatc aaacaggacc tgaattaatc tgcgggtcat tggctcagca
     4441 cgctgagtcg tcgtcctgtt tgccagggtt aaaatcaatt cccggcataa gggcgatatt
     4501 ttcaatgtgc aacatgttgt cggcattgtc actgcgctgg gttcaataaa cagaaaacag
     4561 agttcagcgt tagctgtcac ttgattgcta tgttccaccc cgcccggtat ccaaaccgca
     4621 tattgcggcg gcaccatcca caaagcattt tccaccgtac aggtaattgc accatgtagc
     4681 gccagtatca gctgtccttt gcggtgctga tgtacaggac tggttaactc atccgttccg
     4741 gcgtgaatac aaaatgcgac cgcggcttca tggtgtcggt cgggttcata gccattgaga
     4801 ttcaacctgt gcatcatttt gtccgaattt agcgataatt tgtcatttta gcttgattca
     4861 acataacaat aaaaacggta aggtacagcc tcgtttgtaa caatgagaag catatgacct
     4921 gttcaacttc attaagcggc aaaaacagga ttgtccttat cgctggcatt ctgatgattg
     4981 ccacaacatt acgcgtcacc tttaccggcg cagcaccgtt actggatacg attcgttccg
     5041 cttactcgct gacgacagcg caaaccggct tattgaccac cctgccatta ttggcctttg
     5101 cgctaatatc acctttggcc gccccggtag cgcgacgttt tggtatggaa cgtagcctgt
     5161 ttgccgcgtt acttttgatc tgtgctggta tcgcaattcg ctctctccct tcgccttact
     5221 tattatttgg cggtacagcg gtcattggcg gtgggattgc attaggcaat gtcttactgc
     5281 caggattaat taaacgcgat ttccctcatt ccgtcgccag acttaccggc gcatattccc
     5341 tgacaatggg agctgcggcg gcacttggat cggctatggt agtgccgctg gctttgaacg
     5401 gttttggctg gcaaggcgcg ttgctcatgc tgatgtgttt tcctctgctg gctctttttt
     5461 tatggctgcc acagtggcga agccaacaac atgcaaattt gagtacctcg cgcgccttac
     5521 atactcgggg tatctggcgt tcgccgcttg cctggcaggt cacattgttt cttgggatca
     5581 actcactggt ctattacgtg attattggct ggcttccggc gatcctcatc agtcacggct
     5641 atagcaaagc acaggcgggt tcactgcatg gtttgctgca actagccaca gcagctcccg
     5701 gtttgctgat ccctcttttc ttacatcatg tgaaagatca gcgtggtatt gcagcgttcg
     5761 ttgccttgat gtgcgcagtg ggcgctgttg ggctctgctt tatgccagcg cacgcgatca
     5821 cctggactct gcttttcggt tttggttccg gcgcaacaat gatactgggg ttgacgttca
     5881 ttggtcttcg ggcaagttct gcgcatcagg cggcggcact ctcggggatg gcacaatccg
     5941 tcgggtattt gctggcagcc tgtgggccgc cgctaatggg taaaatacac gatgctaacg
     6001 gtaactggtc cgtaccactg atgggtgttg caatactttc actactgatg gcgattttcg
     6061 gactttgcgc cgggagagac aaagaaattc gctaatatcc ggtgctatag tgacgtgaca
     6121 aatcatgcgt gaaagggaga acaaacacga tgaatattca gtgcaaacgc gtttatgatc
     6181 cggctgaaca gagcgatggt tatcgcgtac tggtcgaccg cctctggccg cgcggtatca
     6241 aaaaaaccga tttagccctt gatgagtggg ataaagaaat cacgccgtca acggaactgc
     6301 gcaaagcctt tcacggcgaa gtcgtcgatt ttgcaacctt tcgcgagcaa tatcttgcag
     6361 aactggcgca acacgagcaa gaaggaaagc ggctggcgga catcgccaaa aaacagccgc
     6421 tgaccctgct ctacgcagca aaaaacacct cgcagaacca tgcgctggtg ctggccgact
     6481 ggctacgtag cttgtgattt tagtacagca tccggcggtt atttttcacc agccggatgg
     6541 tcccgtcgcc acaatgccca ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg
     6601 ctgacccctt gcgctgtcga cactggaatg agcgtcccgc ccttctgctg gcaatagacc
     6661 gacgccggat ttgccatacc aatctgcggc ggtttaggtg cttctggctg agaagaggtt
     6721 gaacaaccag ccaggaccag caagcaaggc aaagcaaaac tgataatttt catttattga
     6781 tctcactcat ttatctacgc ttagagtatc gcggtatcgt tttgttttgc agcactattt
     6841 ttattacatt cactcaaaac atattacgtc ttgtttcatc tttgttggtg atgttttatc
     6901 atgcctgcaa agattaaata atcagcattt acccgccgta tcctggagtt gttttgtgtc
     6961 agatcagatt atcgcccgcg tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct
     7021 ggtccgacag cttctggaga tgctggaaat ggtcactgat atggaatcaa cctacctgac
     7081 caaagtggat gtcgaagcgc gcctgcagca tattatgttt gcccgtaaca gccagaaaat
     7141 gcacatcccg gagaatttta ccgtctcgtg ggattactcg ttatgcaaac gcgccattga
     7201 tgaaaactgc tttttcagcg atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg
     7261 caatcttggc atcaccacat ttctgagcac gccaattcac ttaccggatg gatcattcta
     7321 tggcacgctt tgcgccgcca gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt
     7381 tttacagtta ttcgccggac ttattgcaca atatattcaa aaagaggcac tggttgaaca
     7441 gctgcgcgaa gccaatgctg cgctgattgc gcaatcgtat accgactcgt taaccgggct
     7501 accgaatcgg cgggcgattt ttgaaaatct gacgacgctg ttttccctcg cccggcatct
     7561 taaccataag ataatgatcg cgtttatcga tctggataac ttcaaattaa tcaatgatcg
     7621 ttttggtcat aatagtggcg atctgtttct cattcaggtt ggcgagcgcc ttaatacgct
     7681 ccagcaaaat ggcgaagtta ttggtcgtct cggtggtgat gagtttttgg ttgtttcact
     7741 aaacaacgag aatgcggata tttcgtcgct tcgagaacgt attcagcagc aaatacgtgg
     7801 agaatatcac ttaggtgatg ttgatttgta ttatcccggt gccagtcttg gcatagtaga
     7861 agtcgatcct gaaacaaccg atgcagacag tgccctgcat gctgccgata ttgcgatgta
     7921 tcaggagaaa aaacacaaac agaaaacacc ttttgtcgcg catccagcgc tacattcctg
     7981 aggcgtattc acatcctttt gattggtgat aacatgcgaa tcggtattat ttttccggtt
     8041 gtaatcttca ttacagcggt cgtattttta gcatggtttt ttattggcgg ctatgctgct
     8101 ccgggggcat aaagatgaaa aaaacaacga ttattatgat gggtgtggcg attattgtcg
     8161 tactcggcac tgagctggga tggtggtaac gtcacctcta aaagatagca aaggctgcct
     8221 gtgtgcagcc tttgtgcaat ttacgcgtta acttttaatc ttcctgtaga taaatagcac
     8281 gacaatcgca ccaataacgg caaccacgaa gctgccaaaa ttgaagccat cgactttacc
     8341 aaagccaaac agcgtgctga tccatccgcc gactacagca ccgactatcc ccagcaggat
     8401 agtcataaag aatccaccac catctttacc tggcatgatc cacttcgcca gaataccggc
     8461 aataagccca aaaataatcc atgacagaat gcccattgtt tcctcactta tctgttttgc
     8521 attagcgggt tagtcgctga taaaaagcat agcacaacat cgggagggca agatttgtga
     8581 cgagcatcac ggaggatttt tttgcgatgg cgcagaaatt gcgccatcaa cgatcagtga
     8641 taattaccaa ccacaaacat catgttcgtt ttccgtgtca taagaacgca cggtattcac
     8701 cagatctttt atcacttcag ccgccacttc tggcaccagc aaagtcatcg gcgtctctgt
     8761 ttcataatcg atagaaacgc cattactgtt attggtgacg gtcacggtat acgttgcttt
     8821 gcccatgatt catttcccgt tatgaatgac tttccgttgt tgcgcacctt ccatcaggac
     8881 ttcaggagcc acgaagaagt caatgttgaa ataagtatcg tcagtcatgg cttcaatgtt
     8941 gtgccacttt tctggaggga acaccgcaaa ctgccccgct tcgataagga tcacctgatc
     9001 aggctctgca ctgtgttcat cagcgtagcc gagatatttg accgccccat gcataacgga
     9061 aaggcgtggg taaacccccg ggcgcgttcc tttatcaaga tgacgttcga atatgccggc
     9121 aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta tgaatataat tctgtgggat
     9181 ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa acggtttatt ccagccgttt
     9241 cagggtacgc ctgataattt gcattttaaa taccatttat tggttacttt ttagcaccat
     9301 atcagagaaa aatcagggag gattacaagt gggaagcccg cgacaacgcg ggctgaatgt
     9361 atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac gaacatcaaa ccaatcagtg
     9421 agttgccaac tttagccagt ttctttttgg tacgtatgta ctgcgtgaca aaagcaccag
     9481 aaataatcag gaagctcaaa tagcagaaac tcaccagttc cagcgtcgtc gccagaataa
     9541 agaatgaaat tcccgtatgt ggggcattaa catcgataaa ctgtacgaaa aacgacacat
     9601 agaacaaaat ggctttcgga ttagtcaggc tcaaaattaa cgcgcgttta aaaatggccc
     9661 cgtattgggg ctcatcggat ttggcctcgt tatttttacc cttcagggtc gcgtagagaa
     9721 ttttactccc cagatagagc aaataaaacg caccaagata acgtacaatg ttgaataata
     9781 tcggggtggt cttaattaat gtcgccactc cagcccatgc cagaaacatc aatactgcat
     9841 cgccaataaa tacaccgcag gccgcaagat aaccgccttt cataccgcta ctgacgctat
     9901 ttttgagtac aaacagggta tttggccctg gcaccaacac aataaaaatg gccccaacca
     9961 gataggtcca gtaattcaga accccgtatt cagcgaacac attaacctct ttaattatct
    10021 ttcgatcatg cgcgattaaa ggtgaatatg ctaaccaatc tgtagcggct tagaaaggag
    10081 aaaatcaggt tttaacccga tatcaacccg ataattgaat aattaatcag gcatgctcca
    10141 gtgaaaaatt cgggtagtgc tcggcaaaat actggcgtaa aaattctacc gttatccgca
    10201 ctttcgctga cgtcgccagc cttgaaacat aaacggccca gacgttcgct ggctggtaat
    10261 attccggtag cacttgcact aaatgaccac tggcaatgtt ttcgctaaca tcccaccagg
    10321 aacgcagagc aatcccctgc ccatcgagac accactgatg gacaatttct ccatgattag
    10381 acgacagtgg gccggtgact ttaatcgcat ggtggccttc tttattgcgt aattgccaga
    10441 caccaaaagg atgatcgcgc tctttaatga ccaggcacgg caatgcagaa agatcggtta
    10501 aatgtttcgg cgcaccatgt tgcgcaataa attccggcga cgcgcagaga atgcggtaat
    10561 tagtcgccag tttgcgggcg attaaattag gtgcgatgtc atcgccaatg cgaatatcga
    10621 gatcgacacc ttcattgacc aaatcgacca accggtcttc cacatcaaaa cgtaattcca
    10681 gttgcggata cgccttcgcc agcgctgata acgccggagc caccacctgt cgtccaaaac
    10741 caaagctgct gataatacgc agcatcccct ggggcacctg acgcacgtca gaaagttcgt
    10801 ccatcatctg accgacatcc tgcaaaatcc gctgcgccca ttcataaatc cgctctcctt
    10861 cttcggtaat ggtgacgcgg cgggtggtgc ggtgtaacaa caccacgttt agcgtttgct
    10921 ccagcaaggc gatgcgcttg ctgacgaacg ccggtgaaac gcccagttct tccgccacgg
    10981 cggcaaaacc agcccggcga gccaccagca taaagacgcg caaatcgttc agcagcggta
    11041 aattattcat gattcgtgtt ttatgtttca ccagttacgg ggattaattc cttttcagtc
    11101 aattatagga tggtgatgtt gtcaattttg atggtcagga agtgagaacc caatgatgaa
    11161 aacgatgcgt attgctgcga tcccgggaga cgggattggc aaagaagtcc ttcctgaagg
    11221 gattcgcgtg ttacaggctg ccgctgagcg ctggggcttc gccttgagtt ttgagcaaat
    11281 ggagtgggcg agctgcgagt attacagcca tcacggtaaa atgatgccgg acgactggca
    11341 tgagcaactt agccgtttcg acgccatcta ttttggtgcc gtcggctggc cggacaccgt
    11401 tcctgaccat atttcgttgt ggggttcgct gctgaaattt cgtcgtgaat tcgaccagta
    11461 cgtcaacctg cgcccggttc gtctctttcc tggcgttccc tgcccgctgg cgggaaaaca
    11521 gcctggcgac atcgattttt acgtggtcag ggaaaacacc gaaggcgaat attcctcgct
    11581 cggcggtaga gtgaatgaag gtacagagca tgaagtcgtc attcaggaat cggtatttac
    11641 gcgtcgtggt gtcgatcgca ttttgcgtta tgccttcgaa cttgcgcaaa gccgcccacg
    11701 taagacgcta acttctgcca ctaaatcaaa cggtttagcc atcagcatgc cgtactggga
    11761 tgagcgagtg gaagcaatgg ccgagaatta cccggagatc cgctgggaca agcagcatat
    11821 tgatattctc tgcgcgcgtt ttgtgatgca gccggaacgc ttcgatgtgg tggtggcgtc
    11881 caatttattt ggcgatatcc tttccgatct tggcccggcc tgcaccggca ccattggcat
    11941 tgccccatcc gccaacctga atccggaacg cactttcccg tcgctcttcg agccagtcca
    12001 cggttccgcg ccggatatct acgggaaaaa cattgctaac cctatcgcca cgatttgggc
    12061 cggtgcaatg atgctcgatt ttctcggcaa tggcgatgag cgtttccagc aagcgcataa
    12121 cggtattctg gcagcaattg aagaagtgat tgctcacggg ccgaaaacac ctgatatgaa
    12181 aggcaatgcc accacgccac aggttgccga cgcgatttgc aaaattattt tgcgttaagg
    12241 tcaaaccagt ttatttgaac cgcgtcactg acgcggtttt tttattcgtt ctttgcagta
    12301 aataacctgc gtcatttcac cttttattgt ttccgtttcg tgttttatgg ctttccgtat
    12361 tcttaattgt ttaatttatg taacatgcaa ataatcaatt tgttaaacac aacatacaac
    12421 tcattaccct gcaaataatt acaactggca aaagccagag acgggctttt atgcccagga
    12481 gatgactcat gatgagcaat gttaagaaaa aagatgtgcc gctgataagc atcagcctgg
    12541 tggccattct tttcatcgca gctgcattaa gccttttccc acaacaatcg gccgacgcgg
    12601 ccaacgcaat atacactttt gttacgcgta cgttaggttc cgccgtacag gtattggttt
    12661 tgctggcaat gggactggtg atttatttag ccaccagtaa atacggcaat attcgtcttg
    12721 gcgaaggaaa accggaatac agcacgctct cctgactgtt tatgtttatt tgtgccggtt
    12781 taggttcttc tacgctttat tggggggttg ctgaatgggc ctattattat caaacgcctg
    12841 gattaaatat cgcaccgcgt tcacaacagg cactcgaatt tagcgtccct tactccttct
    12901 tccactgggg catcagcgcc tgggcaactt atacgctggc ctcattaatc atggcttatc
    12961 actttcatgt gcgtaaaaac aaaggtctga gtctttccgg cattattgcc gcgattaccg
    13021 gcgttcgccc gcaaggccca tggagaaaac tggttgattt gatgttcctg atcgccactg
    13081 tcggcgcact gactatttcc cttgttgtca ccgcagcaac ctttactcgt ggactttccg
    13141 cgctgaccgg tttacccgat aacttcaccg tgcaggcatt tgtgatcctg ctttccggcg
    13201 gcattttttg cctaagctcg tggattggta tcaacaacgg tttgcaacgt ctgagcaaaa
    13261 tggttggctg gggcgcgttc ctgctgccat tactggtgct gattgtcggc ccaaccgaat
    13321 ttattaccaa cagcatcatc aatgccatcg gtctgaccac gcaaaacttc ctgcaaatga
    13381 gcttattcac cgatccgctt ggcgatggtt catttacccg caactggacc gttttctact
    13441 ggctgtggtg gatctcatac acccctggcg tagcaatgtt tgtcacccgc gtttcccgcg
    13501 gtcgtaagat taaagaagtt atctggggac tgatcctcgg cagcaccgtc ggttgctggt
    13561 tcttcttcgg cgtaatggaa agctatgcca ttcatcagtt tatcaatggc gtaatcaacg
    13621 tcccacaggt gctggaaaca ctgggcggcg aaacagctgt acagcaagtt ctgatgtcgt
    13681 tgccagccgg taaattgttc ctcgccgcat acctgggcgt gatgattatt ttccttgcct
    13741 cgcatatgga tgcggtggcc tacaccatgg ctgcgaccag tacgcgtaat ctccaggaag
    13801 gtgacgatcc tgaccgtggg ctgcgtcttt tctggtgcgt ggtgatcact ctgatcccgc
    13861 tttccatctt gtttaccggt gcttcgctgg aaacgatgaa aaccaccgtc gtgctcacag
    13921 cccttccctt cctcgtcatt ttactggtga aagtcggcgg atttattcgc tggctgaaac
    13981 aggattacgc cgacattccg gctcatcaag ttgaacatta tctcccgcag acaccggttg
    14041 aagccctgga aaaaacgcca gtgctccctg cgggaaccgt attcaaaggc gacaactgag
    14101 cgccatcatc ctaacgataa aggtatccct atgagcaatc tgagccctga ctttgtacta
    14161 cccgaaaatt tttgcgctaa cccgcaagag gcgtggacca ttcctgcccg tttttatacc
    14221 gatcagaacg cgtttgaaca cgaaaaagag aacgtcttcg ccaaaagctg gatttgcgtc
    14281 gctcacagca gcgaactggc gaatgccaat gattatgtga cgcgtgagat cattggcgaa
    14341 agcatcgtgc tggtacgcgg tcgtgataag gttttgcgcg cgttctataa cgtgtgtccg
    14401 caccgtggtc atcagttgtt gagcggtgaa ggaaaagcaa aaaatgtgat tacctgcccg
    14461 tatcacgcat gggcatttaa actcgatggc aacctggccc atgcacgtaa ctgcgaaaac
    14521 gtcgccaatt tcgatagcga caaagcgcaa ctggttccgg tgcgtctgga agaatatgcc
    14581 ggattcgtct tcatcaacat ggaccccaac gccaccagcg tagaagatca attacccggc
    14641 ctgggcgcga aagtgctgga agcctgcccg gaagtccacg atctgaaact ggcggcccgc
    14701 tttaccaccc gcacgcctgc caactggaag aacattgtcg ataactatct cgagtgctat
    14761 cactgtggtc cggcgcatcc aggtttctcc gactccgtac aggttgatcg ttactggcac
    14821 accatgcacg gtaactggac gctgcaatac ggtttcgcca aaccgtccga acagtcgttt
    14881 aaatttgaag agggtacgga tgcggcattc cacggtttct ggctgtggcc gtgcacgatg
    14941 ctgaacgtca ccccgatcaa agggatgatg acggtcattt atgaattccc ggtggattct
    15001 gaaactaccc tgcaaaacta cgatatttac ttcaccaatg aagagttaac cgacgagcaa
    15061 aaatcgctga ttgagtggta tcgcgatgtg ttccgtccgg aagatttacg tctggttgaa
    15121 agcgtacaga aagggctgaa atcgcgtggc tatcgtggtc aggggcgcat catggccgac
    15181 agtagcggta gtggcatttc cgaacatggt atcgcccatt tccataatct gctggcgcag
    15241 gtgtttaagg actaatgaca tcggcggcgg tattttccgc cgctgggctg atttttgatg
    15301 gagtacagca atgtcagact atcaaatgtt tgaagtacag gtgagccagg ttgaacccct
    15361 taccgaacag gtgaaacgct tcacgctggt ggcaaccgat ggcaaaccat tacctgcgtt
    15421 taccggagga agtcacgtca ttgtgcagat gagcgatggt gataaccagt acagcaatgc
    15481 gtattcacta ctgagttcgc cgcatgacac ctcttgttat cagattgccg ttcggctgga
    15541 ggaaaactcg cgcggcggtt cccgcttttt gcatcagcag gtaaaagtgg gcaatcggtt
    15601 aacgatttca acgcctaata acctgtttgc gctaattccc tcagccagaa agcatctgtt
    15661 tatcgcgggc ggtattggta tcaccccttt cctgtcgcac atggcagagc tgcaacacag
    15721 cgacgtcgac tggcagctac attactgctc gcgaaatcca gaaagttgcg catttcgtga
    15781 tgagctagtc cagcatccgc aggctgagaa agttcacttg catcactcat caaccggaac
    15841 acgactggaa ttagcgcgat tattggcgga tatcgaacct ggcacacacg tttatacctg
    15901 tggtcccgag gcgctaaatg aagcggtaag aagtgaagct gcgcgtctgg acatcgccgc
    15961 cgatacgctg cactttgagc aatttgctat cgaagacaaa accggcgatg cctttaccct
    16021 ggtgcttgcc cgttccggaa aagagtttgt ggtgccggaa gagatgacta ttttgcaggt
    16081 tattgaaaat aataaagccg cgaaagtgga atgtttatgt cgtgaagggg tatgcggaac
    16141 ctgcgaaaca gcaatactgg aaggtgaagc tgaccatcgg gatcagtatt ttagcgatga
    16201 agagcgtgcc agccagcaaa gtatgttgat ctgttgttcg cgtgcgaagg gtaaacgcct
    16261 ggtgttggat ttgtagtttg ccaaagccgg atgtggcgct gagcgcgccc agtccggctt
    16321 cggaagattt tactgcggat attcctgcaa taaattgtgt aatgcttccg ccatcagctc
    16381 accacgccag ccggaaatca gctccggcaa attgttctgc ggtttcagtt tccagtgcca
    16441 gttcagcagt tggttgattt gccgacgcga tgccagcaat tcggcgctga tcttatgcgt
    16501 ttcgctcacg tcagtaatca gcgacttaat cgctttaaac gctttacgat aaccaggcat
    16561 gtccatcagg ttaagcatcg gctgcggtaa ggcctcttcc ggcaatgtct gcgctttttc
    16621 caccagcgcc agcagcgttt taccgtgaaa gcggatttcg ctaccggata aacccaggct
    16681 gtccagttcg cctaaacttc ccggcatata acgcgcgacc gaccacaaat gctcttcacg
    16741 cacgacaaag ttcaccgcca gatcgcgctc tcgcgccttg cgcagtcgcc agtcggctaa
    16801 cagttgcaga caggccagtt ggcgtgtgcg taattgccag gcattggtga tatcacgcca
    16861 ggcatcttcc ggcgcaacga cttcctgacg acgcatttgc atcaggcggc attcatccag
    16921 cgccgcaggt agccagccgg aggcctccgt ttctaccata agcttggcgg tgatcggtaa
    16981 cagataccag acatccgccg ctgcgtattc acactgacgt tcggtcagcg gtctggccag
    17041 ccagtcggtg cgcgattcac tcttgtccag cgtaacgccg gaatactctt ccaccatgga
    17101 agcgaaaccc catgacatcg ggcgtccgca gaaggcagca aggatttgcg tgtcaatcaa
    17161 gggttgtggt aattcgccaa agacattgag gaacacttcc agatcttcac tgcctgcatg
    17221 gagaaatttt gtgatggacg gatcgcgcag gatcgctttc agcggtgacc agtcggtgat
    17281 cccgagtgga tcgattagcg ccagatgctc gccatcgaaa agttgaatca accccagctg
    17341 cgggtaataa gtgcgcgtac gaacaaattc agtatccagg gctatcgccg gaaaggcacg
    17401 gacggcttca cacaaagaag ccagcgcatc gtccgtggta atcatttggt aattcaaatt
    17461 gttttctctt tagtgggcgt caaaaaaaac gccggattaa ccggcgtctg acgactgact
    17521 taacgctcag gctttattgt ccactttgcc gcgcgcttcg tcacgtaatt ctcgtcgcaa
    17581 aatttttccg acgttagatt tcggtaactc atcacgaaac tccaccagct tcggtacttt
    17641 gtatcccgtg agctgacggc ggcaaaaagt caccagtgac tcttcggtaa gcgatggatc
    17701 ttttttcact acgaagattt tcaccgcttc accactggag ccggaaggta cgccaacagc
    17761 cgcgacctcc tgtacgccag gatgctgcat gacgacatct tcaatctcgt tgggatagac
    17821 gttaaaaccg gaaaccagaa tcatgtcttt tttacgatca acaatgcgca ggaatccttc
    17881 ttcatccatt accgcgatgt cgccggtgtg taaccagcca tttttgatga tttcatcggt
    17941 agcatcggga cgctgccagt aacccagcat cacctgcggt cctttgacac aaagctcacc
    18001 cggttgacct ggtggtactt cattatcatc atcatccacc agtttggctt ccgtcgacgg
    18061 caccggcaaa ccgatgctac cactatgata atcaatatca tatgggttaa cgctgaccag
    18121 cggcgcacac tcggtaaggc catagccttc cagcagatac tgtcctgtca gtttcaccca
    18181 acgctctgcc accacttgct gcactggcat ccctccgcct gcggaaagat gcagactgga
    18241 gaaatccagc tgctggaact ctttattgtt cagcaacgca ttgaacaagg tgttaacgcc
    18301 cgtgatagcg gtaaacggat atttcgctaa ctcttttacc aaccctggaa tatcgcgcgg
    18361 gttagtgata agcaggttct gcccacccag ttcgataaac agcaggcagt taatggtcag
    18421 ggcaaaaatg tgatacagcg gcagtgccgt tactaccagt tctttacctg gatgcagcaa
    18481 cggcccatag gtggcgttga cctgttccag gttcgccagc atattgcggt gagtcagcat
    18541 cgcgcctttc gccacacctg tggtgccgcc ggtgtattgc aggaaggcta aatcttccgg
    18601 caccagttcg ggtttgacgt actgcatccg gtagccgtta tgcagtgcgc tacgaaatga
    18661 aatggcatct ggcagatggt atttcggcac caaacgcttg atgtatttaa caacgaaatt
    18721 gactaccgtg ccttttgccg tagatagctg atcgcccata cgggtcagaa ttacgtgctg
    18781 aacggcggtt ttatcaacca ctttttccag tgtgtgagca aagttagaca cgataacaat
    18841 cgccgatgcg ccgctatcgt taagctgatg ctcaagctca cgcggggtat acaacgggtt
    18901 aacgtttacg acgatcatcc cggcacgcaa aatgccaaac agcgccaccg gatattgcaa
    18961 taaattaggc atcatcaacg caacgcgatc gcctttcttc agccccaacc cttgttgcaa
    19021 ataagcggca aacgcgcgac tgcgttcttc cagcttgcgg aaggtcatta cctcccccat
    19081 attcacaaac gcaggctgat cggcgtagcg cgcgaccgac tgctcaaaca tatctaccag
    19141 agattgataa cggtcagggt tgatctccgt cggaacgtcc gcgggataac ggttaagcca
    19201 aaccttcttc aattcttcac ctctaaaatg cgtgttcgtc gtcatcgcaa ccccaaatga
    19261 tatacatgcc gttaacataa tattaactca tcataccagc ttgataatta cccaacgaaa
    19321 aggttgcgaa gcgcgtcact atttattttt atctttaccg taagaatgca gaaacagcgg
    19381 accagccgct gtttcttttt ctttaaaaac aagcaattat tcagttacaa ctgtttgtac
    19441 tctcgcggga ccgggattat accagcccca tccgccgtag ccatagggcc agccacgacc
    19501 gccataaaac catggatcaa tcggctgagg cggcataatc acctgctggg ttaaatgcca
    19561 acgtttgtaa cccgttactt gcatcaccat aaatttatag ggcgtattgc cgattttgcc
    19621 gtcaaccgca ccagtgattg gcccgactac cgtaaccagt tgtccacgga aatccaccgg
    19681 gtccagaaaa ccgttcacat cggcataaat gcgaccgcga gaaggttctc ccagcgtcgg
    19741 tctggctccg ctgtccagcg gtacggtagc aatttccagg cggtttttcc cttgctggtt
    19801 ttgtaccgca accactttgc caccaaagcg tgcctcctga ccaacgtaca gctgcggcgc
    19861 actcatcacc cgaactaaat cttgttgcgg cgtgggactg ctgcctttaa tggcgtccgg
    19921 cacagtgaca caaccgctca gcattagcgc aaacgtacct gccagtatgc ctttgataac
    19981 attcttttga accgccatga tgcgactcct ttttttctca gggcatactc ttaagattca
    20041 ttctttgccc ggaagtttct tccatgcgac gttgttacgt aaataaaccg gttcggcatg
    20101 ttccaccgcc accgttttac cctcggcaaa catctgacac gcaatcggca gcatatcttc
    20161 cgccgctggc agtaaaactt ctccgtcgcg caaaaccagc ccgctctctt taccgagatc
    20221 cggccaggct tgccagcccg tccctaccgt tacccattcg ccggaaagct gttgcattcg
    20281 ttcatgaacg agttcgggtt tgagtacggc ttcggtttct tcaccgtgcc agataccgtt
    20341 ttcatcacgc tgatattcgg cccagtaaac ttcgcccatt cgcgcgtcaa tggctgccag
    20401 cacgcgggtt gcgccgtttt tgcgccacgc cccttgcgcc atcgtcatta gcgtggagac
    20461 gccaatcatc ggtagctctg cgccaagtgc cagcccttgc gcaatgccaa taccaatgcg
    20521 cacgccggta aagctaccgg ggccgcgccc gtaagccaga gcgttaatat cagtcaggga
    20581 agttccgctg gtggtcagga tatcctgcac catcggtaag attcgttgag tatgttcacg
    20641 agggcaaagc tcaaaatgag cgttgacagt accgtcgttc cacagggcga cagagcaggc
    20701 ctctgtcgcg gtatcgatag ccagaattcg catgggtctt cgtgcttaga tcaataaaaa
    20761 ggcgcgcatc ataccatact ccgtaacaaa ttacctggag gatggtatcg caaggaaacg
    20821 aaccgcacgg gcaatgtcac gagtgcgtgg cgcgggcggc agactggcga gaaacgtcgc
    20881 gccgtaagga cgcatcacca gccgattgtc acaaatcacc agcacgccac gatcgtcggc
    20941 gtcgcgaatc agtcgccctc ccccctgttt gagagtaatg acggcatctg gtagttgcac
    21001 ttcatcgaac gggtcgccac cgcgcaaacg acaatcttcc atgcgcgctt ttaacagtgg
    21061 atcatccggc gaggtaaacg gcaatttgtc gataattacc aatgacaatg tatcgccacg
    21121 cacgtccacc ccttcccaga aactactggt tgccacaaga agcgcgttac cggcgctgac
    21181 aaattgctgc aacagttgcc ctttgctggt ttccccctgc aacaaaacgg gaagcgtcat
    21241 ggtagcgcgg aactgctcgg ccagatcacg catcatggcg tgcgaggtac aaagcataaa
    21301 acaacgaccg ttgttagctt cgatgatcgg tcgcagcatt gccgctaact ggcgagcaga
    21361 acctggctgg ttggtttgcg gcagattgcg cggcacacag agtaacgcct ggcggctgta
    21421 atcaaacggg cttggcaata gcaacgactc cgcctgttct atcccaagcc gcgaggtgaa
    21481 atgatgcaga tcgtcgttca ccgacagcgt tgctgaggtg aagatccagc taccaggctt
    21541 ttgcgccatt aactctttga atttatccgc cacgctgagc ggcgtgagag ccagagtaaa
    21601 atggcgcgaa gtgcattcgt accagtagct gtagcccggc tgattgatct cttttagccg
    21661 cttcagccgt gtgcgataca acgtagcgcg ctcaaatgcc gcatccagca aggcggaacg
    21721 ccccagtgac agtttcgcca cgtcataaca aagttccagg gtgtcatcga gcagtaaaaa
    21781 tgcccgctga atttgcggat tagctaacag ctcacgcagg ttaccgcgat aacctggctc
    21841 accgagttgc agacgaaaat cctgcgcact ctgggcaaga cgatcagcgc acttttgtaa
    21901 ctgctgggtg tcttttaatt cggtgcggta agcgatggtg atgtcttttg ccaggtcgag
    21961 cagttgtcga ctggagagtg actgaccaaa atactggctg gcaatgtccg gtagctggtg
    22021 ggcttcgtcg aagatcatga cgtccgcttc cgggatcaac tcgccaaatc cactctcttt
    22081 aaccaccata tccgccagaa agagatgatg gtttaccacc accacatcgg cgtccatcgc
    22141 ttttttacgt gctttgacca caaagcaatc tttatacatc gggcagtcgc tgccaagaca
    22201 gttgtcgttg gtgctggtaa ccagcggcca cgcctgtgaa tcttccgcca cgctgacgca
    22261 ggtgctgata tcaccatcga ctgtttgatt agaccaggag cgcagcagga tcacatcgct
    22321 taagatttgt accggcagat cgccccccgc cagcgcctgc tgttcgagac gttcgaggca
    22381 gaggtagttt gagcgccctt tcagcagcgc cacgttgccc gtatatttca atgcctttga
    22441 gactgttggc aaatcgcggc tgtagagctg atcctgcaac gcttttgagc cggtcgagat
    22501 aatgactttc tttttcgccc gcagcgcagg agccaggtaa gcgtaggttt tgcccgtacc
    22561 ggttcctgct tccaccacca gcggctggcc tttttctatc gcctgggtga cggctaccgc
    22621 catctgtcgc tgtggttctc gcggcttaaa gcctggtatc gctttcgcca gctgaccgtc
    22681 tggtgcaaaa tcgtccgtca cactaccccc tgttgatttg aacagggatt atgtcaggat
    22741 gagggcgctt tcgccagttg aagtggtgac ggcaacctca cattgtggca gtctttgcag
    22801 cacgaaaatg gaagtataat gaggacaaaa tgatgactat cgttcgtatc gatgctgaag
    22861 cccgctggtc tgatgtagta atccacaaca acacgctcta ctacactggt gtaccggaaa
    22921 acctcgacgc cgatgctttt gagcaaaccg ccaacacgct ggcgcagatt gacgccgtgc
    22981 tggaaaaaca gggcagcaat aaatcgagca ttctggatgc caccattttc ctggctgata
    23041 aaaacgactt cgcggcgatg aataaagcgt gggatgcctg ggttgtggcg ggtcatgcgc
    23101 cggtgcgctg cacggtgcaa gcggggctga tgaacccgaa gtataaagtt gaaattaaga
    23161 ttgtggctgc ggtgtaagct ttatcgaagc aaaataagtc agacgataat ttatcgataa
    23221 tactggtcgg ttttacat
//
LOCUS       DAZKIV010000067        23180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZKIV010000067 DAZKIV010000000
VERSION     DAZKIV010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.067"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            190..1326
                     /locus_tag="ACHOT6_002548"
     CDS             190..1326
                     /locus_tag="ACHOT6_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HFP9947743.1"
                     /translation="MYKIRLQKNITILFIFIVMLSFSYWLANSFGMKWGVLAIIGFFL
                     GFTLSFSRFGIVAGWRDMLTMSNSYYVRIHLITIALEVVLFTLCLSFSHAFFGGKMVG
                     NITAISIPFLVGAFVFGIGMQIAGACASGTLYCCGEGRPAFWFVLCCYGIGTLIGNHY
                     KPVLKPIFPGEIITTKDITGGLWSGMLLSLVFILILYLVFSYIEKKRTGKISKIITSG
                     GSFWKDNRFTVLSGGLIIAVLNSLVLAMHGSAWTITGAIYDTSLHTASFLGVLSDNSV
                     ITKPLFQNPMVGMFVGGLLGAAMATVLTGKRIKEPLTRGSIMAGITGGLLMGTGGAYA
                     SCNLGGFFDGTASGSLHGWLWMFMALAGSVIGINLRRFFICSRC"
     gene            1638..2030
                     /locus_tag="ACHOT6_002549"
     CDS             1638..2030
                     /locus_tag="ACHOT6_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001694413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9947744.1"
                     /translation="MRDIQQVLERWGAWAANNYEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGKTFMALARKHGCSDGHIGKRLQKA
                     EGVVEGMLMMLDVKLEMDRYVERDSVTA"
     gene            2378..2584
                     /locus_tag="ACHOT6_002550"
     CDS             2378..2584
                     /locus_tag="ACHOT6_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000399369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947745.1"
                     /translation="MEGVVSQEQAGHRASKNNRFVLFFSQGKNSTGINPSSGGISRQH
                     DEQAAQPDTASRRYYDFRNTFSKA"
     gene            3579..4403
                     /gene="dinD"
                     /locus_tag="ACHOT6_002551"
     CDS             3579..4403
                     /gene="dinD"
                     /locus_tag="ACHOT6_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HFP9947746.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYLAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQQKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            4695..5312
                     /gene="yicG"
                     /locus_tag="ACHOT6_002552"
     CDS             4695..5312
                     /gene="yicG"
                     /locus_tag="ACHOT6_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFP9947747.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(5309..6991)
                     /gene="ligB"
                     /locus_tag="ACHOT6_002553"
     CDS             complement(5309..6991)
                     /gene="ligB"
                     /locus_tag="ACHOT6_002553"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFP9947748.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTEKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            7249..7872
                     /gene="gmk"
                     /locus_tag="ACHOT6_002554"
     CDS             7249..7872
                     /gene="gmk"
                     /locus_tag="ACHOT6_002554"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFP9947749.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            7927..8202
                     /gene="rpoZ"
                     /locus_tag="ACHOT6_002555"
     CDS             7927..8202
                     /gene="rpoZ"
                     /locus_tag="ACHOT6_002555"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFP9947750.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            8221..10329
                     /gene="spoT"
                     /locus_tag="ACHOT6_002556"
     CDS             8221..10329
                     /gene="spoT"
                     /locus_tag="ACHOT6_002556"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFP9947751.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            10336..11025
                     /gene="trmH"
                     /locus_tag="ACHOT6_002557"
     CDS             10336..11025
                     /gene="trmH"
                     /locus_tag="ACHOT6_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFP9947752.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            11031..13112
                     /gene="recG"
                     /locus_tag="ACHOT6_002558"
     CDS             11031..13112
                     /gene="recG"
                     /locus_tag="ACHOT6_002558"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFP9947753.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLAAGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(13097..13963)
                     /locus_tag="ACHOT6_002559"
     CDS             complement(13097..13963)
                     /locus_tag="ACHOT6_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947754.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(13966..15171)
                     /gene="gltS"
                     /locus_tag="ACHOT6_002560"
     CDS             complement(13966..15171)
                     /gene="gltS"
                     /locus_tag="ACHOT6_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFP9947755.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            15451..16842
                     /gene="xanP"
                     /locus_tag="ACHOT6_002561"
     CDS             15451..16842
                     /gene="xanP"
                     /locus_tag="ACHOT6_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFP9947756.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            16963..18672
                     /gene="yicH"
                     /locus_tag="ACHOT6_002562"
     CDS             16963..18672
                     /gene="yicH"
                     /locus_tag="ACHOT6_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFP9947757.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGVDIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQAPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDIRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQHWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVQ"
     gene            complement(18724..21042)
                     /gene="yicI"
                     /locus_tag="ACHOT6_002563"
     CDS             complement(18724..21042)
                     /gene="yicI"
                     /locus_tag="ACHOT6_002563"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFP9947758.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQGNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACTWYTDKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWS
                     VLKETVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     EVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPVYVRDNTLLALGNNDQRPDYAW
                     HEDTAFHLFNLQDGHETVCEVPAADGSVIFTLKAARTGNAITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEHGLVVTPQGNALTITL"
     gene            complement(21052..22434)
                     /gene="yicJ"
                     /locus_tag="ACHOT6_002564"
     CDS             complement(21052..22434)
                     /gene="yicJ"
                     /locus_tag="ACHOT6_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFP9947759.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            22727..22821
                     /locus_tag="ACHOT6_002565"
     tRNA            22727..22821
                     /locus_tag="ACHOT6_002565"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22761..22763,aa:Sec,seq:tca)
     gene            22900..23061
                     /gene="rhuM"
                     /locus_tag="ACHOT6_002566"
                     /pseudo
     CDS             22900..23061
                     /gene="rhuM"
                     /locus_tag="ACHOT6_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         5888 a         6020 c         5780 g         5492 t
ORIGIN      
        1 atcaattcgc tgcattatct cacagaaagt acttgagcgt catattgacg gttatttata
       61 ggtgagtatc attctggaac aggcattttg ggtaacgtgt tcaggatatt gcgtagatac
      121 atttctggaa cgattccttt tcagcaagta catcagcgca tcacgtggaa acatataggt
      181 atattaaaaa tgtataaaat aaggcttcag aaaaacataa ctatattatt tatttttatt
      241 gtcatgctgt catttagtta ctggcttgct aatagttttg gtatgaagtg gggcgtactt
      301 gcaattattg gttttttctt gggttttaca ttgtctttta gtcgatttgg gatagttgca
      361 ggttggagag acatgctgac catgagcaac tcatactatg taaggattca cttaattacc
      421 atcgctcttg aggtggtttt gtttacactc tgcctgtcat tcagccatgc tttttttggc
      481 gggaagatgg ttgggaatat tacagcaata agtatccctt ttctggtggg tgcgtttgtt
      541 tttggtattg gaatgcaaat tgccggagca tgtgcgtcag gaacactcta ttgttgtggt
      601 gagggccgtc cggcattttg gtttgttttg tgctgctatg gtatagggac attaattggc
      661 aatcactata agccagtgct aaaaccaatt tttcccggag aaattattac caccaaagat
      721 attactggcg gcttgtggtc aggcatgtta ttaagtcttg tatttatatt gatactatac
      781 cttgtttttt catacattga gaaaaaacgc accgggaaaa tatctaaaat tatcaccagt
      841 ggtggttctt tctggaaaga taacagattt actgttcttt ctggcgggtt aattatagct
      901 gtactgaact cactggttct ggcaatgcac ggttcagcat ggacaattac cggagccatc
      961 tacgatacat ccttgcatac tgcatcattt cttggggttt tatctgataa ttctgttatt
     1021 actaaaccat tatttcagaa tcctatggtt gggatgttcg taggtggatt gctgggggct
     1081 gcaatggcta ctgtactaac gggaaaaagg atcaaagagc ctctgactcg tggaagcata
     1141 atggcaggaa taactggtgg attgcttatg ggaacaggag gagcgtatgc ttcctgtaat
     1201 ttgggaggat tcttcgatgg aactgcatca ggaagtttac atggatggtt gtggatgttt
     1261 atggctctgg ctggcagtgt aattgggata aatcttcgga gattttttat ttgttcacgc
     1321 tgttaaaaac aaataattga atcctgacag gaaactgtgg gaagaattct attatcagaa
     1381 agtaaagccc ccttgtttta aatgtataca agggggatgc atgctgaaaa agcgtactat
     1441 caaaaaacag taactacgcg acgtagtact acctactcta gaaaaattag ctgagcaacc
     1501 gactatctga aatatactca caacctacga aattttcgta ttttaacctc cgtccggtat
     1561 ataccccgaa ttacggggat gtacggagtt actgtacgga gttaccgttg ccaataatta
     1621 ttcgcaaggt gatgttgatg cgtgatattc agcaggttct ggaacgatgg ggggcgtggg
     1681 cggcaaataa ctatgaggat gttacatggt cgcccattgc tgccggattt aagggactga
     1741 tccccgaaaa agtaaaatca cgtccacagt gttgtgacga tgacgcgatg attatatgcg
     1801 ggtgtatggc tcgccttaac aggaacaaca gcgatctgca tgacttgctg gttgattatt
     1861 acgtgttggg gaaaacgttt atggcgctgg cacgtaagca tggatgctct gatggacata
     1921 tcggaaagcg gctacagaaa gcggaaggtg ttgttgaagg catgctgatg atgctggatg
     1981 tgaagctgga gatggatcgg tatgttgagc gcgattcagt gacggcataa cgcgcataaa
     2041 aaaaaccgca gtcatgagga aactgcggcg gagaagtcag acttgccaat caatcatgaa
     2101 aaacgtaatc gcctgataat tatgccacga agcacggaaa aacagtactg gtgctaagac
     2161 atgaaccatt tgcattgcaa taagttaagc cgaaatgcgt cgggtcctga atggtgataa
     2221 tcaccacgca gacaatcaaa gctaccataa cgaacatcgt tacagttgga gcttttaccc
     2281 cgttggggaa aaggtgcaca tctggatcat cattatggtg atcgagggta tgtaaagccc
     2341 accagcctga ttaaaggtta accggaaaaa gccaggtatg gagggagtgg ttagccaaga
     2401 acaagccgga caccgcgcca gtaaaaacaa cagattcgtg cttttcttca gccagggtaa
     2461 gaactccacg gggattaatc cgtcgtctgg tggaatcagt cggcagcatg acgaacaggc
     2521 cgcgcagccg gacaccgcca gcagaaggta ttatgatttt cgtaatacct ttagcaaagc
     2581 gtgaatcatc attacggtag ggatggtcat aatgaccaca cctccccccc accggaacag
     2641 ggaggatgac ctacgaaaat ttcgtaaaat tcccccccaa atcccccacg gttgcccgtt
     2701 aagtgcaaga gcacaggaac gacctcgcta atcagttacc agcgctcacg ctcaaatatc
     2761 cacaggaacg gggctaaatc gcattctggc gcaatgttca ctatcggggc aagcattcac
     2821 cggaaaacgt catacaggct tacaggcggc ttaaaattct atgggcggat aacagggcgg
     2881 gatcgggagg cttacgaaaa tttcgtaacc cccctacaca aaaagtgcag gttactaccc
     2941 gaaaagtgta gtgccattgc tgggggctaa aaaaaaccgc agtgtgataa cactacggca
     3001 cgccaccaaa gaaacataac aaggaatcag caatgagtaa tacctgtaag ccttcggggt
     3061 gattgtaaca catgagcaat cttttactgg aagctgtttt tcactggatc acgtatcgtt
     3121 atggggattc ccatatcgga gagcatccac cagcttcata acggataacc gaaaaatgat
     3181 aacggttaac ctggaaaaaa ttccggtgaa tatttgccct gacagttccc atatcggcgg
     3241 cacacctgaa aaaaccgtgt acacaaggat gtacacaata aaaatcgtta ttacagaaat
     3301 cccagtaatg acaagccttt gatgcagata ctcaatgagc agattcaaca ttgaataact
     3361 cgtaacgcca attcttactt ttcagccttc acaaatgccg cccactcaaa cagagcggca
     3421 tttttcttcc ccgcaacttc aattctgttt tgcgtgcctg ctccagattt tgcgatgttt
     3481 ttttgcccag cacactgaga acgtgagata ctcacaactg tatataaata cagttacaga
     3541 tttactttct ttgcaatcga tatcacatgg agtgggcaat gaacgaacat catcaacctt
     3601 ttgaagaaat acggcattat ggcactgaag ggcaagaatt ttggtcagct agagaattgg
     3661 caccgttact ggattatcgg gactggcgca attttcaaaa ggtactggcc cgcgcgactc
     3721 aagcgtgcga agccagcaat caagctgcat ctgaccattt cgttgaaacc accaaaatgg
     3781 ttgtattagg ttcaggggct caacgagaac ttgaagatgt tcatctctcc cgctatgcct
     3841 gttacctggt agtacaaaac ggcgaccctg cgaaaccggt tattgcggca gggcaaactt
     3901 atcttgctat ccagacccga cggcaggagc tggctgacga tgaagctttc aggcaacttc
     3961 gtgaagacga aaagcgtctt tttctgagaa atgaattgaa agaacataac aaacaattgg
     4021 ttgaggcagc gcagcaagct ggggtagcga cagctatcga tttcgccata tttcagaatc
     4081 atggttaccg ggggctgtat ggtggattag atcagaaagc catccatcag cagaaggggc
     4141 tgaaaaagag tcagaagatc ctggatcata tgggttcaac agaactggcg gctaatctct
     4201 ttcgagctac ccaaacagaa gaaaaactca agcgggatgg cgtgaattca aaacagcaag
     4261 ctaataccac acactttgac gtgggtagca aggtgaggca aaccattcag gaacttggcg
     4321 gaaccatgcc tgaggagttg ccgaccccgc aggtcagcat caagcagttg gaaaacagtg
     4381 taaaaattac agagaagaaa tagcagaaac taaaaactct ttttgttgat tgagataccc
     4441 gatgcgtaag ccaaagtcca ggtgcggtga acacaatggc taaacattgc acctttcttt
     4501 tccacccctc aattttaacc tattttttct tatgcatttt ctcagacaag aagtcagaag
     4561 aatgcatcta cgctacagaa aatagcgatt tcacataact acaagttatc aatttcctct
     4621 caccttaaaa aatctcaatc gtgacaatac gcacaaatcg ctaccctgcc agacagattt
     4681 ttagggagag aaccatgctg ttacacattt tgtatttggt tggcattact gccgaagcca
     4741 tgacgggggc tctggcggcc gggcgacgcc gcatggatac atttggcgta attattattg
     4801 cgaccgccac cgcaattggc ggagggtcag tgcgcgatat tctgctgggc cactatccgc
     4861 tcggctgggt caaacacccg gaatatgtga ttatcgtcgc taccgccgca gtactcacca
     4921 ctatcgttgc tcctgtaatg ccttacctgc gcaaagtgtt tctggtgctc gatgcgctcg
     4981 gactggtggt cttttctatc atcggcgcac aggttgcact ggatatgggg cacggcccaa
     5041 ttattgccgt tgtcgcggcg gtaaccaccg gtgtatttgg cggcgttttg cgcgatatgt
     5101 tctgtaaacg cattccgctg gtctttcaaa aagagttgta tgccggtgtc tcatttgcct
     5161 ccgccgtgct gtacatcgcg ctgcaacact atgtttctaa ccatgatgtg gtgattatct
     5221 ccaccctggt attcggcttt tttgctcgtt tactggcgct acgtcttaag ctgggattac
     5281 cggtctttta ctacagccac gaaggtcact aaggttcaaa acctgtgatc tgctgggcag
     5341 ccagccaact gcccagcttc ttgatttgcg cattttcctt ccattcaata acctgtctgg
     5401 cgcgcccaga tccagtcccc ggcagctgct gccagaactg ctccgtgcta aataaaagtt
     5461 gcgaccagga ccgttcatca ctggcattaa gcgccgcccg ggttagcggt attcccattg
     5521 ccatcaccca gcgagtaaaa ggctggttac gagccagatt aaactgatgc cataactgcg
     5581 cacttttact tttcgcgatc cccggcgtgt tctgtaattg ctctggcgtt aataaaagcc
     5641 aggaaaagat atgctcaaag cgatgagtct gatgcagcgc gcgccaaccg gcctcaccaa
     5701 tgccatccag ccccagaacc tgttttgacc ccagccagac taagcgtgaa atgaactgtt
     5761 cctgacaaac atcagaagca aagtagcagg tcaacgagtt aaagcggttt tctggtggtg
     5821 tcggttttgt acgttctgca ccgcgccaga ccacatcatc aatgcgagga ataccctgac
     5881 cggcaaggct gacgaggatc tgatcaccag gcgcaatatc ccactcctgc cagcgcctga
     5941 cggaaccaat attcacccgc tggacttttt tatcatccag catgacaggt gcgagtgacg
     6001 caaccaccga tattttaccg ctcttaccca ccgcaaactg aatcgccttc acttcggcaa
     6061 cctgagctac aggttgatat ttccaggcca ccagccactc tgcctggccc ggtagccaat
     6121 ggcgggattc tggctctttc gccgctcgta caactacgcc atcggtgacg aagggtaatt
     6181 tcgctttcca ccactcattg cgtacgcgcg caacctcatc agcatttttc accgcacggg
     6241 tatacctctg cgttagagta aaacctgcgg tagccagctc ctttaaacga tcggtcatta
     6301 actgcggtcc atccggccat gcccagacaa aaacagccag tgagtttagc gtgtcgctat
     6361 tcccctggcg catcatcagg ccagcaactt ttgcgcgggc atttattccc cccatttgtt
     6421 gttggatatg cccctcgcgc tggagaaata tttccccctg aagcgtactg ttggctaaag
     6481 gcccgctaac ggtttgcggc acagcggaaa ttaagctaac tttctccgtc cagtcctcgc
     6541 ctttcaggcc attaccgcga ctgattgctt tgttcagttt cccgtcccga taaaccaggg
     6601 ttaccgcaac gccatcaact tttggctgca cccaaagatc gctacgttct cgcatccaca
     6661 gacttaatgc gtttttatcc accattttgc gtacacccgt atgggcaacc ggatgcataa
     6721 ccgcaccatt taaaggcggc atcatcacgt cgcggggctc gctcccaaag cagcgttgcc
     6781 actgcgtaag acgggcgctt aactgatcgt aaacaccgtc ttccacctca ctttttcctt
     6841 ccttccagta gtcatcgtcc cactgtttta tttgctgttg caggcgggaa atttcttcct
     6901 gtgctctggc tggcgaccag gccggacaga ccgcccacac agatgattgc cagcacaaga
     6961 tacttattaa tatcgccatc catactttca tcatcacctc cgctgtagat agtcaggcag
     7021 atatacaacg tggtgaaggc aaagccgagt ggcaaaaacg gagtctgcga ggacgcttcc
     7081 tgagaatcgt ctttattgca gtgaatgaca ggcaaatgcg gaaacagcta cgtaaaacgc
     7141 aacaactttg cgcaaaaagt gtgagcaagg gctacgtcac atggccgcgc cgtgtataat
     7201 aagctcgtat gtaggcttta tttcgctaat cacatacgaa agatactcat ggctcaaggc
     7261 acgctttata ttgtttctgc ccccagtggc gcgggtaaat ccagcctgat tcaggcttta
     7321 ttaaaaaccc aaccgttgta tgacacccag gtttctgttt cacacaccac acgccaaccg
     7381 cgtcctggtg aagtccacgg tgaacattat ttctttgtta atcatgatga atttaaagaa
     7441 atgattagca gagatgcgtt cctcgaacac gcagaagttt ttggtaatta ctatggcact
     7501 tcgcgtgagg ccattgagca agtactggcg accggtgtcg atgtttttct cgatatcgac
     7561 tggcagggcg cgcagcaaat tcgccagaaa atgccgcacg cgcggagtat ctttatttta
     7621 ccgccgtcca aaattgaact ggaccgccgt ctacgcggtc gcggtcagga cagcgaagag
     7681 gtcattgcaa agcgtatggc gcaagctgtt gcagaaatga gccattacgc cgaatatgat
     7741 tacctgattg tgaatgatga cttcgatacc gcgttgaccg atttgaagac cattattcgc
     7801 gccgaacgtc tgcgcatgag ccgccaaaag cagcgtcatg acgctttaat cagcaaattg
     7861 ttggcagact gaacctgatt tcagtattat gcccagtcat ttcttcacct gtggagcttt
     7921 ttaagtatgg cacgcgtaac tgttcaggac gctgtagaga aaattggtaa ccgttttgac
     7981 ctggtactgg tcgccgcgcg tcgcgctcgt cagatgcagg taggcggaaa ggatccgctg
     8041 gtaccggaag aaaacgataa aaccactgta atcgcgctgc gcgaaattga agaaggtctg
     8101 atcaacaacc agatcctcga cgttcgcgaa cgccaggaac agcaagagca ggaagccgct
     8161 gaattacaag ccgttaccgc tattgctgaa ggtcgtcgtt aatcacaaag cgggtcgccc
     8221 ttgtatctgt ttgaaagcct gaatcaactg attcaaacct acctgccgga agaccaaatc
     8281 aagcgtctgc ggcaggcgta tctcgttgca cgtgatgctc acgaggggca aacacgttca
     8341 agcggtgaac cctatatcac gcacccggta gcggttgcct gcattctggc cgagatgaaa
     8401 ctcgactatg aaacgctgat ggcggcgctg ctgcatgacg tgattgaaga tactcccgcc
     8461 acctaccagg atatggaaca gctttttggt aaaagcgtcg ccgagctggt agagggggtg
     8521 tcgaaacttg ataaactcaa gttccgcgat aagaaagagg cgcaggccga aaactttcgc
     8581 aagatgatta tggcgatggt gcaggatatc cgcgtcatcc tcatcaaact tgccgaccgt
     8641 acccacaaca tgcgcacgct gggctcactt cgcccggaca aacgtcgccg catcgcccgt
     8701 gaaactctcg aaatttacag cccgctggcg caccgtttag gtatccacca cattaaaacc
     8761 gaactcgaag agctgggttt tgaggcgctg tatcccaacc gttatcgcgt aatcaaagaa
     8821 gtggtgaaag ccgcgcgcgg caaccgtaaa gagatgatcc agaagattct ttctgaaatc
     8881 gaagggcgtt tgcaggaagc gggaataccg tgccgcgtca gtggtcgcga aaagcatctt
     8941 tattcgattt actgcaaaat ggtgctcaaa gagcagcgtt ttcactcaat catggacatc
     9001 tacgctttcc gcgtgatcgt caatgattcc gacacctgtt atcgcgtgct cgggcaaatg
     9061 catagcctgt acaagccgcg tccgggtcgc gtgaaagact acatcgccat tccaaaagcg
     9121 aacggctatc agtctttgca cacctcgatg atcggcccgc acggtgtgcc ggttgaggtc
     9181 cagatccgta ccgaagatat ggatcagatg gcggagatgg gtgttgccgc gcactgggct
     9241 tataaagagc acggcgaaac cagcaccacc gcgcaaatcc gcgcccagcg ctggatgcaa
     9301 agcctgctgg agctgcaaca gagcgccggt agttcgtttg aatttatcga gagcgttaaa
     9361 tccgatctct tcccggatga gatttacgtt ttcacaccgg aagggcgaat tgtcgagctg
     9421 cctgccggtg caacgcccgt cgacttcgct tatgcagtgc ataccgatat cggtcatgcc
     9481 tgcgtgggcg cacgcgttga ccgccagcct tacccgctgt cgcagccgct tactagcggt
     9541 cagaccgttg aaatcattac cgcaccgggc gctcgcccga atgccgcgtg gctgaacttt
     9601 gtcgttagct caaaagcgcg cgccaaaatt cgtcagttgc tgaaaaacct caagcgtgat
     9661 gattctgtaa gcctgggccg tcgtctgctc aaccatgctt tgggtggtag ccgtaagctg
     9721 aatgaaatcc cgcaggaaaa tattcagcgc gagctggatc gcatgaagct ggcaacgctt
     9781 gacgatctgc tggcagaaat tggtcttggt aacgcaatga gcgtggtggt cgcgaaaaat
     9841 ctacaacatg gggacgcctc cattccaccg gcaacccaaa gtcacggaca tctgccaatt
     9901 aaaggtgccg atggcgtgct gatcaccttt gcgaaatgct gccgccctat tcctggcgac
     9961 ccgattatcg cccacgtcag ccccggtaaa ggtctggtga tccaccatga atcctgccgt
    10021 aacatccgtg gctaccagaa agagccagag aagtttatgg ctgtggaatg ggataaagag
    10081 acggcgcagg aattcatcac cgaaatcaag gtggagatgt tcaatcatca gggtgcgctg
    10141 gcaaacctga cggcggcaat taacaccacg acttcgaata ttcaaagttt gaatacggaa
    10201 gagaaagatg gtcgcgtcta cagcgccttt attcgtctga ccgctcgtga ccgtgtgcat
    10261 ctggcgaata tcatgcgcaa aatccgcgtg atgccagacg tgattaaagt cacccgaaac
    10321 cgaaattaat gttttatgaa cccaacacgt tatgcacgca tctgcgaaat gctcgccagg
    10381 cggcagcctg atctgaccgt ctgcatggag caggtccaca aacctcataa cgtttctgcg
    10441 attattcgta ccgcagatgc cgttggcgta catgaagttc acgccgtctg gcctggtagc
    10501 cgcatgcgca ccatggcttc ggcagcggcg ggtagtaaca gctgggtaca ggtgaaaaca
    10561 caccgcacca ttggcgatgc cgtcgctcat ctcaaaggcc agggcatgca gattctggca
    10621 acccatcttt ctgataacgc tgtcgatttc cgcgaaattg attacactcg cccgacctgc
    10681 attttgatgg gacaggagaa aacgggcatc acgcaggaag cattggccct ggcggatcag
    10741 gacatcatca ttccgatgat cggcatggtg cagtcgctga atgtttccgt tgcctcagcc
    10801 ctcattcttt acgaagccca gcgtcagcgg caaaatgcag gcatgtacct gcgtgaaaac
    10861 agcatgttgc cggaagcaga gcaacaacgc ctgttgtttg aaggcggcta tccggtgctg
    10921 gcgaaagtcg caaaacgcaa aggcctgcct tatccccacg tcaatcagca aggcgagatc
    10981 gaagctgatg ccgactggtg ggctactatg caggctgcag ggtaagtgcc atgaaaggtc
    11041 gcctgttaga tgctgtccca ctcagttccc taacgggcgt tggcgcagca cttagtaaca
    11101 aactggcgaa aatcaacctg cataccgtgc aggatctact cttacacctt cccctgcgct
    11161 acgaagatcg cacccatctc taccccatcg gagaactact gccgggcgtt tatgccacgg
    11221 tggaaggcga agtgctgaac tgcaatatct ccttcggcgg tcggcggatg atgacctgcc
    11281 agatcagcga cggttccggc atcctcacca tgcgcttttt caatttcagc gcggcaatga
    11341 aaaatagcct ggcggcgggc cgccgtgtac tggcttatgg cgaagcaaag cgcggtaaat
    11401 atggtgcgga gatgatccac ccggaatacc gcgtgcaggg cgatctcagc acgccagaat
    11461 tacaggaaac gctcacgccg gtttatccaa caacggaagg cgtaaagcag gccacgctgc
    11521 gtaaattaac cgaccaggcg ctggatctgc tcgacacctg cgccattgaa gaactcctgc
    11581 cgccggaact gtcacaagga atgatgacgc taccggaagc gttgcgcact ttgcaccgcc
    11641 cgccaccgac gctacagctt agcgatctgg aaaccgggca gcatccggcg caacgtcgtc
    11701 tgattctgga agaactgctg gcgcacaacc tcagcatgtt agccttacgt gccggagcac
    11761 agcgttttca tgcccagccg ctgagcgcca atgacacgct gaaaaataaa ctcctcgccg
    11821 ccttaccgtt caagcaaacg ggcgcacagg cacgcgtagt ggcggagatc gagcgcgata
    11881 tggcgctgga tgtgccgatg atgcgtctgg tgcagggcga tgtaggttcc ggtaaaacgc
    11941 tggtcgccgc cctcgccgcg ttgcgtgcga ttgcccacgg caaacaggta gcattgatgg
    12001 caccaaccga attactcgcc gagcagcacg ccaataactt ccgcaactgg tttgaaccgc
    12061 tcggtatcga agtgggctgg ctcgccggta agcagaaagg caaagcgcga ctgtctcagc
    12121 aggaagccat cgccagcggt caggtgcaga tgattgttgg tacgcacgcc atcttccagg
    12181 aacaggtgca gtttaacggt ctggcgctgg tgattatcga cgaacaacat cgctttggcg
    12241 tgcatcagcg tctggcgtta tgggagaaag gccagcagca aggcttccat ccgcatcagt
    12301 tgatcatgac cgccacgccg atcccccgca cgctggcaat gactgcgtat gccgatctcg
    12361 atacctcggt gatagatgag ctgccgccag gccgcacgcc agtgactacg gtcgctattc
    12421 ctgatacccg ccgtaccgac atcattgacc gcgtgcgcca cgcctgcata actgaaggtc
    12481 gtcaggcgta ctgggtttgt acgttgattg aagagtcgga attgctggaa gcgcaggcgg
    12541 cggaagctac ctgggaagag ttgaaactgg cgctaccaga gttgaacgtt ggcctggtac
    12601 acgggcggat gaaacctgcc gagaaacagg cggtgatggc gtcgtttaaa caaggtgagc
    12661 tacacctgct ggttgccaca accgttattg aagtcggcgt tgatgtgcct aacgccagtc
    12721 tgatgattat cgaaaacccg gagcgtctgg gtctggcgca gttacaccag cttcgcgggc
    12781 gtgtaggtcg tggtgcggtg gcttctcact gcgtgctgct ctacaaaacg ccgctctcca
    12841 aaaccgcgca aattcggctg caagtgctgc gcgacagcaa cgacggtttt gtgattgcac
    12901 agaaagactt agagattcgc ggaccgggtg aactgttagg tacgcgtcag acgggtaatg
    12961 ctgagtttaa agtggcggat ttactgcgcg atcaggcgat gatcccggaa gttcagcgcc
    13021 tggcgcgaca tattcacgaa cgttacccac aacaagcaaa agccctgata gaacgctgga
    13081 tgccggagac ggaacgttac tccaacgcat gaccagactc atcacaagac aagtcacgca
    13141 gtaaatcttc aatctggata gtgaggatat ccattgcctc atacgcatcc tgtgcaattc
    13201 gcgccgccat tgcgccttcc ggttcaccac gcagaatttc agccgctgcc tgtacgccag
    13261 ccacaatcat ttcaggtgtt aacatttggc gaagaaaagc gcgattatca gaggaccagc
    13321 catctttatg gcctaatgga tgcccccagc ctatccattc ttgcgaccat aacggattgg
    13381 cttctatggg ataaattaga tgcgcggcat actgaatagc acacaccgct tttcctggcg
    13441 tatccagcgc ccaccagcgc gaccagagag aacgaattaa cggcgcggcc ccacccagag
    13501 tattgaacac cgccagccag caaagaacgt tattgaaacc acgctcgtta tccatacgct
    13561 gcaacgcggt gtccagtaaa aattgccgta cctgctggcg ttgggaagca ttcatcatct
    13621 cctgccagag atagggccga cacaatgcct tcattaagga ttcttcgaag tgatcaccat
    13681 aaccgccagc taaaacggtt tcatgccatt gcgccaggca gagcggaaac agatagcgaa
    13741 acaactctat ttgcatcggt tcgttataaa cgagattgag tacatagtaa gcactcaaat
    13801 cccattcgct gatctgctgc cactctttag ccgccagcgc cttgagttcg gcatctgcgt
    13861 aatcaaattc cgtttcccat acacagagca ctggcggctc atcaccaaaa atatggcgac
    13921 agtgttcacg tagcgtttcc ggttccttcc gacgcttttt catttttaac cggcaaaaat
    13981 cggcaacatt aaatacagct taatcaccag cgcattgacg atatcgataa agaacgcacc
    14041 gaccatcggc accaccaaaa acgccatgtg cgacgggcca aagcgttcag tgatcgcctg
    14101 catgttggcg attgccgttg gcgttgcacc gaggccaaaa ccacagtgac ccgcagccag
    14161 cactgccgca tcgtagtttt tgcccatcat gcgccaggta acgaagatgg catacaacgc
    14221 catgaagatg gtctgtacca ccagaatcgc cagcatcggc agcgccagcg aagccagctc
    14281 ccacagtttc agccccatca gcgccatcgc caggaacaac gacaggctta cgttacccag
    14341 cacggatacc gcacgctcaa agacgcggta aaagcccatc attgacagac cgttgctcag
    14401 aatcacgcca acaaacagta cacagacgaa ggtcggcagt tcaaaagcag tgccagccaa
    14461 aagttgcgca acaattttcc ccaccgtcag gcagatagca atcagcgcga tagtttcaat
    14521 cagcaccagc gaggtgatca tgcggcccac atccggcttt tcaaacgccg tcgggacttc
    14581 ctggtcatcc ggaataccgt tcggcgtggt ggagtgtttc accagatagc gcgccaccgg
    14641 accgccaatc aagccgccca gcaccagacc aaacgtcgca caggccatcg ccacttccgt
    14701 cgcattggtg aagccatagc gttcaatgaa caatttactc cacgcagcgc ccgtaccgtg
    14761 accgccggaa agcgtaatag aaccggccaa cagccccatc agcggatcaa gccctaacag
    14821 gctagccata ccaatgccaa tggcattttg catcaccaac agaccaacaa ccacaatcaa
    14881 gaagatgcca accacacgcc caccggcacg caaactggca atgttggcgt tcaggccaat
    14941 ggtggcgaag aatgccagca ttaacggatc gcgcagggac atatcaaagt tgacttccca
    15001 gcccatgctt tttttcagta ccagtagcgc cagcgccacc aacaaaccac ccgcaacagg
    15061 ttccggtatg gtgtatttct tcaaaaagga gacggaatgg accaacttac gcccgagcag
    15121 caacgtcagc gttgcggcaa caagcgttgc taaagtatcg agatgaaaca tagttactcc
    15181 tttgtatccg catcactctt tcatactcgt catacttcat cgcgcagtgg caacttcatg
    15241 aactatgacg ggtatcgagt tatgctttcc tgggtcaaat tctcagctgt taatgaacaa
    15301 atggcatgga ttttaagcag aaaaatacca aaagttatat aaaaaagtgc atttatctca
    15361 tttaaatatt tagatgagga tgtggcaaac gtttgctttt ctctttcggt cagctaaaat
    15421 gcccgctttg ctaccacggg attgttttcg atgtctgttt ccaccctcga gtcagaaaat
    15481 gcgcaaccgg ttgcgcagac tcaaaacagc gaactgattt accgtcttga agatcgtccg
    15541 ccgcttcctc aaaccctgtt tgccgcctgt cagcatttgc tggcgatgtt cgttgcggtg
    15601 atcacgccag cgctattaat ctgccaggcg ctgggtttac cggcacaaga cacgcaacac
    15661 attattagta tgtcgctgtt tgcctccggt gtggcatcga ttattcaaat taaggcctgg
    15721 ggtccggttg gctccgggct gttgtctatt cagggcacca gcttcaactt tgttgccccg
    15781 ctgattatgg gcggtaccgc gctgaaaacc ggtggtgctg atgttcctac catgatggcg
    15841 gctttgttcg gcacgttgat gctggcaagt tgcaccgaga tggtgatctc ccgcgttctg
    15901 catctggcgc gccgcattat tacgccgctg gtttctggcg ttgtggtgat gattatcggc
    15961 ctgtcgctaa ttcaggtggg gctgacctcc attggcggcg gttacgcagc catgagcgat
    16021 aacaccttcg gcgcaccgaa aaatctgctg ctggcaggcg tggtcttagc cttaattatc
    16081 ctgcttaacc gtcaacgtaa cccttactta cgcgtggcct cactggtgat tgcgatggcg
    16141 gccggatatg cgctggcgtg gtttatgggc atgttgccag aaagcaacga accgatgacg
    16201 caagaactga ttatggtgcc aacgccgctc tattacggtc ttggcattga atggagtctg
    16261 ctgctgccgc tgatgctggt ctttatgatc acttcgctgg aaaccattgg cgatatcacg
    16321 gcgacctctg acgtttccga acagccagtg tccggtccgc tgtacatgaa acgcctgaaa
    16381 ggcggcgtgc tggcaaacgg cctgaactcg tttgtttcgg cggtgtttaa caccttccct
    16441 aactcctgct tcgggcagaa caacggagtg atccagttaa ccggtgttgc cagccgctat
    16501 gtcggttttg tcgtcgcgct gatgttgatc gtgctgggtc tgttcccggc agtgagcggt
    16561 tttgtacaac acattcctga accggttctg ggcggcgcaa cgcttgtaat gtttggcacc
    16621 atcgccgcct ccggtgtgcg tatcgtttct cgtgagccgc tgaaccgtcg ggcgattctg
    16681 attatcgcgc tgtcgctggc ggttggtctg ggcgtgtctc agcagccgct gattttgcag
    16741 tttgcccctg aatggctgaa aaacctgctc tcctccggga tcgccgcggg cggtattact
    16801 gccatcgtgc tgaatctgat tttcccacca gaaaaacagt aattcactgg cggcggtaag
    16861 ataattaccg ccgcagtctc acctctttca ccattcccgc cttgaggatt gcgcgtaaat
    16921 cgtgcataac tcccttatgt gcatttcacg ggatggaaga ccatgaaatt tattgggaag
    16981 ctgcttctct acattctcat cgctctgtta gtggtgatcg ctggcctcta ttttcttctg
    17041 caaacccgct ggggagcaga acatatcagc gcatgggttt ccgagaatag cgactatcat
    17101 ctggccttcg gggcgatgga tcaccgtttt tccgcgccat ctcatatcgt gctggagaac
    17161 gtcacgtttg gtcgtgatgg ccagcccgcg accctggtgg ccaaaagtgt cgacattgcg
    17221 ctaagtagtc ggcaactgac cgaaccacgc catgtcgata ccatcctgct ggaaaacggg
    17281 acgctgaatc tcaccgacca gaccgcgccg ctaccgttca aagccgatcg tctgcaactg
    17341 cgtgatatgg cgtttaatag cccgaatagc gaatggaaac tgagcgcgca gcgggtaaat
    17401 ggcggcgtag ttccgtggtc accaaaagcc ggtaaagtgc tgggtacgaa ggcacagatt
    17461 cagtttagtg ccggatcgct ttctctcaat gatgttcctg ccactaatgt actgattgaa
    17521 ggcagtattg ataacgatcg cgttacgctg actaacctgg gtgtcgacat cgcccgcggg
    17581 acattaaccg gaaacgcgca gcgtaacgcc gacggcagct ggcaagtgga aaacctgcgc
    17641 atggcggata tacgtctaca aagcgaaaaa tcgctaaccg acttctttgc gccattacgc
    17701 tctgtcccgt cgttgcagat tggtcgcctg gaagtgatcg atgctcgttt gcaagctccg
    17761 gactgggcgg tgaccgacct cgatctcagc ttgcgcaaca tgaccttcag taaagatgac
    17821 tggcagacac aagaaggcaa actgtcgatg aacgctagcg agttcattta tggttcgctg
    17881 catttatttg acccgattat aaacgcggaa ttttccccgc agggcgtagc gctgcgccag
    17941 ttcaccagcc gctgggaagg gggtatggtc agaacgtcag ggaactggct gcgtgacggg
    18001 aaaacgttga tccttgatga tgcggcaatt gccggactgg aatatacctt gccgaaaaac
    18061 tggcaacagt tgtggatgga aacgacaccc ggttggttaa acagcctgca actgaagaga
    18121 tttagcgcca gccgcaatct gatcattgat atcgaccctg acttcccgtg gcagctcacc
    18181 gcgctcgatg gttacggtgc caacctgacg ctggttaccg atcataaatg gggcgtctgg
    18241 agtggctcgg cgaatctgaa tgccgccgcc gcgacgttta atcgtgttga tatccgtcgt
    18301 ccgtcgctgg cgctaaccgc caacagcagc acggtgaata tcagcgaact gagtgcattt
    18361 actgaaaaag gcattctgga agccaccgcc agtgtttcac aaacgccaca acgtcagacc
    18421 catatcagcc tgaatggacg cggtgtgccg gtgaatattt tgcaacattg gggatggcct
    18481 gaattaccgt tgactggcga cggcaatatt cagcttaccg ccagtggcga tattcaggcc
    18541 aatgtcccgc tgaaacctac ggttagcggg caattgcatg ccgtgaacgc cgcaaagcag
    18601 caagtgactc aaaccatgaa tgcgggtgtc gtttccagcg gcgaagttac atcgacggag
    18661 ccggtgcagt aaacatcaat ctcgccgggt acgactcaga cgtacccggc attcttcaat
    18721 agattacaac gtaattgtca gcgcattccc ttgaggcgtc accaccagcc catgctcact
    18781 ttcagcctgc gaaccacctt gcagaccatt tactttcaca acattgcgca agcacagtgt
    18841 ccagttcttc gcctcgcctg caccagtcac agtaatcgcg ttgccagtac gtgctgcttt
    18901 taaagtaaag atcaccgaac cgtcagcagc gggcacttca cagacggttt catgcccgtc
    18961 ttgcagatta aagaggtgga atgccgtgtc ttcgtgccac gcgtaatcgg gacgttgatc
    19021 gttgttgccc agcgccagta gggtgttatc acgcacataa acgggcagac tcaggaagct
    19081 gtgctgctgt ttatgccagc gactaccgtc gagctcatcg ttgtgccaca ggtgtgtcca
    19141 gcgaccttcc ggcaagtaga actgcacctc gcccgcttca gtgaacaccg gagcaaccat
    19201 cacgttgtcg cctagcatgt attgacggtc aaggtaatca caagccggat cgtccgggaa
    19261 ctccatcatc atggcccgca tcatcggcgt accccgcgca ttcgcacgcg cagcttcacg
    19321 atacagatac ggcatcatgc ggcatttcag ttgcgtgaag aagcgcacca catcacagga
    19381 ctcatcatca taggcccacg gcacacgata agatttactg ccatgtagac ggctatggct
    19441 ggagagcaaa ccaaacgcgc accagcgttt gtaaacgtgc gccggagcgg tattctcaaa
    19501 gccgccgata tcgtggctcc agaagccaaa acctgaaagt ccaatagaca aaccaccgcg
    19561 caggctttcc gccattgatt cgtagttagc gtaacagtca ccaccccaat gtaccgggaa
    19621 tttctgcgca ccgacggagg ccgagcgggc aaacaagacg gcttcttcct cgccgacggt
    19681 ttccttgagt acgctccaca ccagttcgtt gtagatgtat gcataatggt tatgcatttt
    19741 ctgcggatcg gaaccgtcaa accactgaac atcggttggg atacgttcgc caaagtcggt
    19801 cttaaagcaa tcaacgccca tcgcgaccag acctttcagt ttgtcggtgt accatgtgca
    19861 ggcatccgga ttggtaaagt cataaattgc cagacctggc tgccatttat cccactgcca
    19921 cagcgaaccg tccgggcgtt tgagtaaata acctttctct tgtaactctt taaagacggg
    19981 ggatttttgc ccaatatagg ggttaatcca gacgcagatt ttcagccctt tcgctttcag
    20041 gcggcggatc atcccttccg ggtccgggaa agtcagcggg tcccactcaa aatcgcacca
    20101 ctggaaggct ttcatccaga aacagtcaaa gtggaaaaca tgcagcggta gattgcgttc
    20161 cgccatacca tcgataaagc tgtttaccgt cgcttcgtcg tagttggtgg taaatgaagt
    20221 ggttagccac agaccgaagg accacgcggg cggcagcgcc ggacgaccag taaagcgggt
    20281 ataacgatca agtaccgctt tcggcgtcgg gccgtcgata acaaagtatt cgagatattc
    20341 actctcaacg ctgaactgca ctttggagac tttctccgat cccacttcaa aagagacgca
    20401 ttggggatga ttgaccagta ccccataacc acggttagtc atgtagaacg ggatattttt
    20461 atacgcctgt tcagtacttg tgccgccgtc ccggttccag gtctctaccg tttgaccatt
    20521 gcgcaccagg gcagtgaagc gctctcccag accataaact gtttcgccaa cgccaagatc
    20581 cagccgctca aacatataat ttcgttgatt attcgtgtcc tgcacgtagc cattattttt
    20641 cacctgacta ccggtaatac gttcgccgtt gcgcagaaaa tccagtgacc agaactcacc
    20701 tttgctgaca cgcgcactta agttgccact tttaaactca gcataacgct ctgtgttttc
    20761 gattgtgacc ttcacgtcct gcaaaatatt gagcggataa tgaggaccgt tattcagcgc
    20821 cccctgaaaa tgctcaatcc gcacaccgac aataccttcc tgtggggaga aaaagcgcag
    20881 cgtaaataaa ggcgtgtcaa gctgccaggt acgttcacgt acatcacggg gggcagcata
    20941 gaccaccatt tcattaccct gctgttcaac ctcgaacacc tgaagcgggt gaatcaaatt
    21001 gaggccaggt tgaatcaacc agtttccatc gctaattttc attgccgttc cttagttctg
    21061 cacttcttga gaggtgaatt gttgctggca acggcgtttg ccctgggcca gctgttccat
    21121 aacggttttc agattgtgcg tcgtgagtga gtagtagcgt ttagcgataa tcgcgctcag
    21181 caaataacag atcgccggaa caatcgtgaa tagcgcaata atgatgctaa tcgtggcgct
    21241 gttctgcgct ttttctgccg catcatatcc gccataagcc agcatccagc cgataagagc
    21301 gccgccgaag gccaacccca gtttgagcac aaacagcgtg ccagcaaaac tgatcccggt
    21361 caggcgttta ccattgcacc actcgccgta gtcgacggta tcggacatca ttacccactg
    21421 gataggtgtc accagttgat gcaacacacc aatcacgaag atgaagacaa acatagtgat
    21481 gctggcctgc atgggaacaa agaacatcgc gaggctaatc actgccagca gggcgttcgt
    21541 ccaccagaag atagtgactt tacatttcca gtcggtcagc ggttttgcca gagcggaacc
    21601 aatcaggtta cccacgcaat aagtggtgag aaaagcgaca aacacttccg gcgtgcccaa
    21661 aatccatgtg acgtaataca tcatcgcccc accgcgtacg cacaccgcca ggatattgaa
    21721 aatggttagt aaaccgacaa tccgccactg gtcgttttgc cagatatcac gtaaatcttc
    21781 ccgcatagac gttgttgtag gtggtgcttc aacgcgttct ttagtggtga agaaacaaaa
    21841 tgccagcatc atgaatgcca ccacggaaag gaccgcgata ccgccctgga aaccgagtgg
    21901 tttattatca ccgccaatta aattaaccag tggcatcatc agaacagtag aaagcatgcc
    21961 tcccgcggtc gccagcacaa aacgccagga ttgcagcgag atacgctgag tcgggtcatt
    22021 ggtgattaca ccacccaatg cgcagtaagg gatattgacg acggtataaa gtaaggtaag
    22081 tagggtgtaa gtaattgctg catagatcat tttgccgttc atactgagat ctggcgtgct
    22141 ataggccagt acacagacga tcccgaatgg cagtgcgcca aacagtaccc acggacgaaa
    22201 tttaccccag cgagagcgcg ttcgatcggc caacaacccc atgcaaggat cggaaatcgc
    22261 atccagtgca cgagcgacca aaaacatggt tccgacaaaa ccggcaggaa tgccaaaaat
    22321 atcggtataa aagaacatca tatataacat tacgttatcg aaaataatgt ggctggcggc
    22381 gtctcccatg ccataaccaa ttttctcttt aacggacaac acttcactct tcatctgctt
    22441 ttccttgatg ctataggggt gctgagaccg ataacgcatt ttgtaaacca tccctgtgag
    22501 agcgaatatt ccgatatctg gttattaatt tatgattctt gttttatgtg atcgtggtag
    22561 cgttaattcc gctcatatat cattgtaaaa tatgggtttt atatgaacta taatgctttc
    22621 gtgataatac gctgcgtgta ttaggcggaa aaaactgatc tgggggatgt agaaactcaa
    22681 ggaagtagct ataatgcgcc ccgcctccat gtagcaatcg aggcgcggaa gatcgtcgtc
    22741 tccggtgagg cggctggact tcaaatccag ttggggccgc cagcggtccc gggcaggttc
    22801 gactcctgtg atcttccgcc aaaatgcctc tcctgacgtt ctccaacccc ttgtaatact
    22861 gtcaatccag atattttcta ctgctcaata ttcaatcggc ttcaggaata cctggtctaa
    22921 ggttttgtga tggacgatga gcggctgaag aatccgcctg tcggttcttc tgtcctcgaa
    22981 cgcatccgcg atattcacgc cagtatgcgc cgtgtttatt tgcaggtgat ttgcccccct
    23041 gctgactctc agccaactgt atcgccatta aggtgaagat gttgttttac tcgccccaga
    23101 acaccctttt ttatacgact attctatttc tttaaccgct ggcgaaaaaa actgactata
    23161 aacttcacca ataatcagac
//
LOCUS       DAZKIV010000068        23030 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZKIV010000068 DAZKIV010000000
VERSION     DAZKIV010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23030
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.068"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..530
                     /gene="ybeT"
                     /locus_tag="ACHOT6_002567"
     CDS             <1..530
                     /gene="ybeT"
                     /locus_tag="ACHOT6_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFP9947760.1"
                     /translation="AIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSPGSLPLTTAA
                     TELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRAQSKLGWIYL
                     KGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQKWLKLAAKQ
                     HYKNAERLLAGLPAH"
     gene            complement(540..1967)
                     /gene="djlB"
                     /locus_tag="ACHOT6_002568"
     CDS             complement(540..1967)
                     /gene="djlB"
                     /locus_tag="ACHOT6_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFP9947761.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEDIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALMLQWCRQHRVDYYPLVVMMIEARDLVNDKGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1964..2671)
                     /gene="ybeR"
                     /locus_tag="ACHOT6_002569"
     CDS             complement(1964..2671)
                     /gene="ybeR"
                     /locus_tag="ACHOT6_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9947762.1"
                     /translation="MDMESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRNYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQ
                     YFAGYTFGRQYWQSPEDDNLQLLREFLARKEYDDSGNDMFYQLFASDNAYYPTLSWQP
                     LAYYSACPETLKDMSDL"
     gene            2835..3812
                     /gene="ybeQ"
                     /locus_tag="ACHOT6_002570"
     CDS             2835..3812
                     /gene="ybeQ"
                     /locus_tag="ACHOT6_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFP9947763.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIREIEDLL"
     gene            complement(3882..4364)
                     /gene="ybeL"
                     /locus_tag="ACHOT6_002571"
     CDS             complement(3882..4364)
                     /gene="ybeL"
                     /locus_tag="ACHOT6_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFP9947764.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4599..7181
                     /gene="leuS"
                     /locus_tag="ACHOT6_002572"
     CDS             4599..7181
                     /gene="leuS"
                     /locus_tag="ACHOT6_002572"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFP9947765.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKVMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7196..7777
                     /gene="lptE"
                     /locus_tag="ACHOT6_002573"
     CDS             7196..7777
                     /gene="lptE"
                     /locus_tag="ACHOT6_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFP9947766.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7777..8808
                     /gene="holA"
                     /locus_tag="ACHOT6_002574"
     CDS             7777..8808
                     /gene="holA"
                     /locus_tag="ACHOT6_002574"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFP9947767.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8810..9451
                     /gene="nadD"
                     /locus_tag="ACHOT6_002575"
     CDS             8810..9451
                     /gene="nadD"
                     /locus_tag="ACHOT6_002575"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFP9947768.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9475..10086
                     /gene="cobC"
                     /locus_tag="ACHOT6_002576"
     CDS             9475..10086
                     /gene="cobC"
                     /locus_tag="ACHOT6_002576"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFP9947769.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARRVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            10346..10663
                     /gene="rsfS"
                     /locus_tag="ACHOT6_002577"
     CDS             10346..10663
                     /gene="rsfS"
                     /locus_tag="ACHOT6_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFP9947770.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10667..11134
                     /gene="rlmH"
                     /locus_tag="ACHOT6_002578"
     CDS             10667..11134
                     /gene="rlmH"
                     /locus_tag="ACHOT6_002578"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFP9947771.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11165..13066
                     /gene="mrdA"
                     /locus_tag="ACHOT6_002579"
     CDS             11165..13066
                     /gene="mrdA"
                     /locus_tag="ACHOT6_002579"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFP9947772.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYASALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            13069..14181
                     /gene="mrdB"
                     /locus_tag="ACHOT6_002580"
                     /gene_synonym="rodA"
     CDS             13069..14181
                     /gene="mrdB"
                     /locus_tag="ACHOT6_002580"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFP9947773.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14192..15280
                     /gene="rlpA"
                     /locus_tag="ACHOT6_002581"
     CDS             14192..15280
                     /gene="rlpA"
                     /locus_tag="ACHOT6_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFP9947774.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVHGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15419..16630
                     /gene="dacA"
                     /locus_tag="ACHOT6_002582"
     CDS             15419..16630
                     /gene="dacA"
                     /locus_tag="ACHOT6_002582"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFP9947775.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16741..17004
                     /gene="ybeD"
                     /locus_tag="ACHOT6_002583"
     CDS             16741..17004
                     /gene="ybeD"
                     /locus_tag="ACHOT6_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFP9947776.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            17105..17746
                     /gene="lipB"
                     /locus_tag="ACHOT6_002584"
     CDS             17105..17746
                     /gene="lipB"
                     /locus_tag="ACHOT6_002584"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFP9947777.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQLQPEATTNNIAPRLLQNILALLNNPDFEYITA"
     gene            18005..18958
                     /gene="ybeF"
                     /locus_tag="ACHOT6_002585"
     CDS             18005..18958
                     /gene="ybeF"
                     /locus_tag="ACHOT6_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFP9947778.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRAPILHGVIDVIRNAF"
     gene            19167..20132
                     /gene="lipA"
                     /locus_tag="ACHOT6_002586"
     CDS             19167..20132
                     /gene="lipA"
                     /locus_tag="ACHOT6_002586"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFP9947779.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20233..20436)
                     /gene="tatE"
                     /locus_tag="ACHOT6_002587"
     CDS             complement(20233..20436)
                     /gene="tatE"
                     /locus_tag="ACHOT6_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFP9947780.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20565..21353)
                     /locus_tag="ACHOT6_002588"
     CDS             complement(20565..21353)
                     /locus_tag="ACHOT6_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFP9947781.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIVPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21446..21829
                     /gene="crcB"
                     /locus_tag="ACHOT6_002589"
     CDS             21446..21829
                     /gene="crcB"
                     /locus_tag="ACHOT6_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFP9947782.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21883..22092)
                     /gene="cspE"
                     /locus_tag="ACHOT6_002590"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21883..22092)
                     /gene="cspE"
                     /locus_tag="ACHOT6_002590"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFP9947783.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22267..22827)
                     /gene="pagP"
                     /locus_tag="ACHOT6_002591"
     CDS             complement(22267..22827)
                     /gene="pagP"
                     /locus_tag="ACHOT6_002591"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFP9947784.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQAWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGQSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5909 a         5782 c         5697 g         5642 t
ORIGIN      
        1 tcgcaatatt tatagttgca gcaattacct gtattagcca acccaagaaa acaacgttgc
       61 gtgataaagc catggtgaat tatgcctttg attatttaag ctcaccaggc agtctaccgt
      121 taaccacggc agccacggag ctttccgcga ttcatggtca ctcaacatcg caatatcgcc
      181 ttggcgaatt ttatcttcat ggtagcgacg gtaaaccact ggattataca caggcgagat
      241 actggtatga gcaatcagca gaacaggaaa atccgcgcgc acaaagcaaa ctggggtgga
      301 tctacctgaa aggcctggga gtcaaacccg atacccgtaa agcaattctc tggtataagg
      361 aagcagctga acaagggtat gctcatgctc aatatacttt gggtttgatc tacagaaatg
      421 gcactggtat taatgttaac cattatgaat ctcaaaaatg gttaaaactg gccgccaaac
      481 aacattacaa aaatgcggaa agattacttg ccggacttcc cgcacattaa cgtcgactgt
      541 cacccgaaga gatggagtat tttccctatc aaaccggcaa agataaaaat gtagaaaata
      601 attttgatga cgcccagaca gcccagcccg ccatcgtcaa cgggcattgg ctgcggttgc
      661 ggctccggct cgacggtttc ctgaccggta aaattcgccg cctgaattaa taatcgacga
      721 ataattaatg cttccagtgg acaacgatct tctgcatcaa ggcggctcac aatcggcatt
      781 aatgattctt ctgtcggttg gctggcctgc actaattgtt tggcaactaa atataatggc
      841 cataagggat gttctgtatc acgcgtgagt gagatccgcg gcttacgtcc tttgtgcaaa
      901 accatctcga ccagtgaacg ccccagcgca gagagttttt catcgctgag tatttcgtat
      961 aaatgaaaac gcgtacgggc gctgtcgccg gggacataaa gtagcggttt tcccttatcg
     1021 ttaaccagat cacgcgcttc aatcatcatc accactaatg ggtaataatc gacccgatgc
     1081 tgacggcacc attgcaacat taaagcatcc gcctgggtgg agggatactc gcgccagtag
     1141 tcacaaagtt ctgccagcaa gctttcgcct tctccacaat agacgcgttg cgcgtattca
     1201 taataacgcg catcttcatt atcaggttca gcttcccgcc acgaccgggc gtaatccaga
     1261 agttcgggga tatccaggcg acctgcgcta taccagcgga gtaaatttct gtgcagtttt
     1321 gcatcatcgg ggatgatcac tgcgcgatgt tgcgctaaat acaatgtcag ccattctgga
     1381 tgcgatgacc aaatccgagc gaacatttgg taaaaatcga tggtgtcatt ttgcacggca
     1441 acgggcagat gcagaatggt ctggaagtta aaaacattcc ccttcgcaat ggcatataaa
     1501 aaggattcca gttcttcttc gtctatttcc ccgacgtttt cctcctgcaa ccaccgcaat
     1561 ctttctgcta ataacaccac gcactcgaag gataaatggg cagtgttcat ggcgattgtg
     1621 cacagcgacc agcgtaattc atcaatatct tccatcgagc aataatttaa ttgctgaatg
     1681 aatcgctgcc aggcggaggg cagaaaacgt tcgctatcag aggcaagtaa cgcgcgaaag
     1741 gcgagcagaa tttcatgttc tgctacctca tattcttctg gttgccaaac agatttagca
     1801 ggcgactgcg caatccggag cgcctcctca tacgcctgac gaagttgttt aaaaccctgc
     1861 ggatcggttt ctggatgaaa ggacggtaac agcgccagat aagcgcggcg gataatatcg
     1921 acgtcagtcg tttcctctat ttcgaggatc ttccagcaat ttttcatagg tcgctcatat
     1981 ccttaagcgt ttccgggcat gcagaatagt aagccaatgg ttgccaggac aaggtagggt
     2041 aatacgcatt atcactggca aataattgat aaaacatatc attgccggaa tcgtcgtatt
     2101 ctttacgcgc taagaattca cgtaataact gcagattatc atcctcggga gactgccaat
     2161 attgccgacc aaaggtataa ccggcaaagt attgccgcca gttacgataa taacgtagag
     2221 cccgtagatg aatgcgcgac tggatccata gactttcttc ttcgctcaac cagttattaa
     2281 gtacgcccat tcggcttaaa aaacccatcc ggacataatc ccaggcttta attcctccct
     2341 caccacagca ttcggcggtg ctggcgacaa attgcgcata agcctgacct tgctcattta
     2401 gttcagcaag atagttacgc cattcacatg gagaataacg aaaccagcgg tgataaaacc
     2461 ctgccaaccg agcggcatga ccgttctcga tcattcggtg aattgtgcgc agcaggtcat
     2521 cacggtcatc aattccccat gaggaagaaa ggtcaaaaaa gcatgtacct aaatacattt
     2581 tgggtgatga atgcgaaggt aaacagcatt cattgcgtat ttccatcgga gtggaaagtg
     2641 caaacaatat tttttgcgac tccatgtcca tataatttat ccttaaatat tcattaaatg
     2701 aatattagcc gcattgcgag gctcgtttat ttatactttt ctgacgcgac ataatacgca
     2761 taaaataagg cttacgcaaa aatggaacac ataaaaatat aatatgtttt atctgcaacc
     2821 ataaggaaat gataatgatt ttcacgtcaa cttgctgcga taatttatca atagatgaaa
     2881 ttatcgaacg tgctgaaaaa ggcgattgcg aggctcaata tattgttggg ttttattata
     2941 atcgcgatag cgcaattgat tctccggacg acgaaaaagc cttttactgg ctgaagctgg
     3001 ccgccgagca aggtcattgt gaagcacaat attccttagg acaaaagtat actgaggata
     3061 aaagccgtca taaagataat gagcatgcca tcttttggct gaaaaaagct gccctacaag
     3121 gccatacttt cgcttccaac gcccttggct ggatactgga tcgtggagaa gaccccaatt
     3181 ataaagaagc ggttgtctgg tatcagatag ccgcggagag cggaatgtct tatgcgcaaa
     3241 ataatcttgg gtggatgtac agaaatggca acggagtcgc aaaagactat gcgctggcat
     3301 ttttctggta caaacaagct gcattacaag gccatagtga cgcgcaaaac aatctggccg
     3361 atctttatga agacggaaaa ggcgttgctc aaaacgagac actcgccgca ttctggtatt
     3421 tgaaaagcgc acagcagggt aatcggcatg ctcagtttca aattgcatgg gattataacg
     3481 ctggcgaagg ggtggaccag gactataagc aagcgatgta ctggtatctg aaggctgccg
     3541 ctcaggggag cgtcggcgct tacgtcaaca tcggttatat gtataaacac ggacaaggcg
     3601 ttgagaaaga ttatcaggct gcctttgaat ggtttacgaa agccgctgaa tgcaatgacg
     3661 ccactgcctg gtataacctg gccattatgt atcactacgg agaaggaaga cctgtcgatc
     3721 tccgacaggc tctcgacctg tatcgtaaag ttcagtcatc cggaaccaga gatgtcagtc
     3781 aagaaattcg tgagattgaa gatttactgt agagataaaa acaagggccg gggaatttca
     3841 ttcggcccat attctcttta ttgctgtgcg aaatcagact attacggctc aaacgggcgt
     3901 ctctggaact ggtcatgacc acatttcggg catagcgtca gcacttccgg tgtgtagatc
     3961 gggagatgga agtgacattt ctcgcagacc agatttccca gcccgaccac ttctccgctg
     4021 tgataaaccc catgatgatt gaggtcctgg aaaacttcgc gccattcaag ctgcgtttta
     4081 tcggtgatgt ctgccagctc ctgccacaag ctttctttaa tcacccgcat aaagacgctg
     4141 tcagattctt ctttcaggct ctcttcatag ctcatggcga actcttccag gtcacgtctg
     4201 acagctcgcg tcagctcatc gacctcggtt cgcgttaact cccctgtttt tattacgcgc
     4261 tcgcgcgcct gttccaccag tgcgtcgata tcacgttcgc cattgcgcag gcgttcgctc
     4321 agtgacgcaa ccagttcacg gtaatattga gcaaccttgt tcatcgtttc atctcccggg
     4381 tcgttaactg actataataa tagacgttct ttgttccttg ctttgctaat acgcccacaa
     4441 actctgtaaa ttaatgtatg aataatccgg gcatcatttc aacgtcgagt gcgcgaaagg
     4501 ctgttttgac gcggacgttt gggctatgct atgcggatct gaaaaaccac ctcaacgcta
     4561 catttgtagc cgtattgaaa acaggaccac tggctgccat gcaagagcaa taccgcccgg
     4621 aagagataga atccaaagta cagcttcatt gggatgagaa gcgcacattt gaagtaaccg
     4681 aagacgagag caaagagaag tattactgcc tgtctatgct tccctatcct tctggtcgac
     4741 tacacatggg ccacgtacgt aactacacca tcggtgacgt gatcgcccgc taccagcgta
     4801 tgctgggcaa aaacgtcctg cagccgatcg gctgggacgc gtttggtctg cctgcggaag
     4861 gcgcggcggt gaaaaacaac accgctccgg caccgtggac gtacgacaac atcgcgtata
     4921 tgaaaaacca gctcaaaatg ctgggctttg gttatgactg gagccgcgag ctggcaacct
     4981 gtacgccgga atactaccgt tgggaacaga aattcttcac cgagctgtat aaaaaaggcc
     5041 tggtatataa gaagacttct gcggtcaact ggtgcccgaa cgaccagacc gtactggcga
     5101 acgaacaagt tatcgacggc tgctgctggc gctgcgatac caaagttgaa cgtaaagaga
     5161 tcccgcagtg gtttatcaaa atcactgctt acgctgacga gctgctcaac gatctggata
     5221 aactggatca ctggccagac accgttaaaa ccatgcagcg taactggatc ggtcgttccg
     5281 aaggcgtgga gatcaccttc aacgttaacg actatgacaa cacgctgacc gtttacacta
     5341 cccgcccgga cacctttatg ggttgtacct acctggcggt agctgcgggt catccgctgg
     5401 cgcagaaagc ggcggaaaat aatcctgaac tggcggcctt tattgacgaa tgccgtaaca
     5461 ccaaagttgc cgaagctgaa atggcgacga tggagaaaaa aggcgtcgat actggcttta
     5521 aagctgttca cccattaacg ggcgaagaaa ttcccgtttg ggcagcaaac ttcgtattga
     5581 tggagtacgg cacgggcgca gttatggcgg ttccgggtca cgaccagcgc gactacgagt
     5641 ttgcctctaa atacggcctg aacatcaaac cagttatcct ggctgctgac ggctctgagc
     5701 cagatctttc tcagcaagcc ctgactgaaa aaggcgtgct gttcaactct ggcgaattca
     5761 acggtcttga ccatgaagcg gccttcaacg ccatcgccga taaactgact gcgatgggcg
     5821 ttggcgagcg taaagtgaac taccgcctgc gcgactgggg tgtttctcgt cagcgttact
     5881 ggggcgcgcc gattccgatg gtgacgctgg aagacggtac cgtaatgccg accccggacg
     5941 accagctgcc ggtgatcctg ccggaagatg tggtgatgga cggcattacc agcccgatta
     6001 aagcagatcc ggagtgggca aaaactaccg ttaacggtat gccagcgctg cgtgaaaccg
     6061 acactttcga cacctttatg gagtcctcct ggtactatgc gcgctacact tgcccagagt
     6121 acaaagaagg tatgctggat tccgaagcgg ctaactactg gctgccggtg gatatctaca
     6181 ttggtggtat cgaacacgcc attatgcacc tgctctactt ccgcttcttc cacaaactga
     6241 tgcgtgatgc aggcatggtg aactctgacg aaccagcgaa acagttgctg tgtcagggta
     6301 tggtgctggc agatgccttc tactatgttg gcgaaaacgg cgaacgtaac tgggtttccc
     6361 cggttgatgc tatcgttgaa cgtgacgaga aaggccgtat cgtgaaagcg aaagatgcgg
     6421 caggccatga actggtttat accggcatga gcaaaatgtc caagtcgaag aacaacggta
     6481 tcgacccgca ggtgatggtt gaacgttacg gcgcggacac cgttcgtctg tttatgatgt
     6541 ttgcttctcc ggctgatatg actctcgaat ggcaggaatc cggtgtggaa ggggctaacc
     6601 gcttcctgaa acgtgtctgg aaactggttt acgagcacac agcaaaaggt gatgttgcgg
     6661 cactgaacgt tgatgcgctg actgaagatc agaaagcgct gcgtcgcgat gtgcataaaa
     6721 cgatcgctaa agtgaccgat gatatcggcc gtcgtcagac cttcaacacc gcaattgcgg
     6781 cgattatgga gctgatgaac aaactggcga aagcaccaac cgatggcgag caggatcgcg
     6841 ctctgatgca ggaagcgctg ctggccgttg tccgtatgct taacccgttc accccgcaca
     6901 tctgcttcac gctgtggcag gaactgaaag gcgaaggcga tatcgacaac gcgccgtggc
     6961 cggttgctga cgaaaaagtg atggtggaag actccacgct ggtcgtggtg caggttaacg
     7021 gtaaagtccg tgccaaaatc accgttccgg tggacgcaac ggaagaacag gttcgcgaac
     7081 gtgctggcca ggaacatctg gtagcaaaat atcttgatgg cgttaccgtg cgtaaagtga
     7141 tttacgtacc aggtaaactc ctcaatctgg tcgttggcta agcgcgggag gaagcgtgcg
     7201 atatctggca acattgttgt tatctctggc ggtgttaatc accgccgggt gtggctggca
     7261 tctgcgtgat accacgcagg ttccttccac tatgaaggtc atgatcctgg actcaggcga
     7321 tccgaacggg ccattaagcc gtgcggtgcg taaccagtta cgtctgaatg gtgtcgagtt
     7381 gcttgataaa gaaaccacgc gtaaggacgt tccatccctg cgtttgggta aagtgagcat
     7441 cgcgaaagat accgcatcgg tattccgtaa cggtcaaaca gcagagtatc agatgatcat
     7501 gacggtgaat gcgaccgtgt tgatccccgg ccgtgatatc tacccgatta gcgccaaagt
     7561 cttccgttcg ttctttgata acccgcaaat ggcgttagcg aaagataacg aacaagacat
     7621 gatcgtaaaa gagatgtacg accgtgctgc cgaacagctg attcgtaagc tgccaagcat
     7681 ccgtgctgcg gatattcgtt ccgacgaaga acagacgtcg accacaacgg atactccggc
     7741 aacgcctgca cgcgtctcca ccacgctggg taactgatga ttcggttgta cccggaacaa
     7801 ctccgcgcgc agctcaatga agggctgcgc gcggcgtatc ttttacttgg taacgatcct
     7861 ctgttattgc aggaaagcca ggacgctgtt cgtcaggtag ctgcggcaca aggattcgaa
     7921 gaacaccaca ctttttccat tgatcccaac actgactgga atgcgatctt ttcgttatgc
     7981 caggctatga gtctgtttgc cagtcgacaa acgctattgc tgttgttacc agaaaacgga
     8041 ccgaatgcgg cgatcaatga acaacttctc acactcaccg gacttctgca tgacgaccta
     8101 ctgttgatcg tccgcggtaa taaattaagc aaagcgcaag aaaatgccgc ctggtttact
     8161 gctcttgcga atcgcagcgt gcaggtgacc tgtcagacac cggagcaggc tcagcttccc
     8221 cgctgggttg ctgcgcgcgc aaaacagctc aacttagaac tggatgacgc ggcgaatcag
     8281 gtgctctgct actgttatga aggtaacctg ctggcgctgg ctcaggcact ggagcgttta
     8341 tcgctgctct ggccagacgg caaactgaca ttaccgcgcg ttgaacaggc ggtgaatgat
     8401 gccgcgcatt tcaccccttt tcattgggtt gatgctttgt tgatgggaaa aagtaagcgc
     8461 gcgttgcata ttcttcagca actgcgtctg gaaggcagcg agccggttat tttgttgcgc
     8521 acattacaac gtgaactgtt gttactggtt aacctgaaac gccagtctgc ccatacgcca
     8581 ctgcgtgcgt tgtttgataa gcatcgggta tggcagaacc gccggggcat gatgggcgag
     8641 gcgttaaatc gcttaagcca gccgcagtta cgtcaggccg tgcaactcct gacacgaacg
     8701 gaactcaccc tcaaacaaga ttacggtcag tcagtgtggg cagagctgga agggttatct
     8761 cttctgttgt gccataaacc cctggcggac gtatttatcg acggttgata tgaaatcttt
     8821 acaggctctg tttggcggca cctttgatcc ggtgcactat ggtcatctta aacccgtgga
     8881 aacgctggcg aatttgattg gtctgacgcg ggtcacaatc atccctaata atgttcctcc
     8941 gcatcgtccc cagccggaag cgaacagcgt gcagcgtaaa cacatgcttg aactggcgat
     9001 tgccgacaag ccattattta ctcttgatga acgcgagcta aagcgcaatg ccccctctta
     9061 cactgcgcaa acactgaaag agtggcggca ggaacaaggg ccggacgtgc cgctggcgtt
     9121 tattattggt caggattcat tgctgacctt tccgacctgg tacgaatacg aaacgatact
     9181 cgacaatgca catttgatcg tctgtcggcg tccaggttac ccacttgaaa tggcgcaacc
     9241 gcaataccag caatggctgg aagatcattt gacacataac ccggaagatc ttcaccttca
     9301 gcctgccggt aaaatttatc tggctgaaac gccgtggttt aacatctcgg cgaccatcat
     9361 ccgcgaacgt ttgcaaaacg gtgaatcgtg tgaggattta ttgccggaac cggtactgac
     9421 ttacattaac caacaaggct tgtatcgctg atacctgtcg ttacggaggg catgatgcga
     9481 ctgtggttaa ttcgtcatgg cgaaacgcaa gcgaatgtcg atggtctgta cagcggtcat
     9541 gcgcccacac ccctgaccgc acgcggtatt gagcaagcgc aaaatctgca tacactgcta
     9601 gatgatgttt cctttgacct ggttttatgc agtgaactgg aacgggcaca gcataccgcg
     9661 cgacgggttc tcagtgaccg ccagctcccc gttcatatca tccctgaact caacgaaatg
     9721 ttttttggcg actgggagat gcgacatcat cgcgacctca tgcaagaaga tgccgaaaac
     9781 tatagcgcgt ggtgcaatga ctggcagcat gctatcccca cgaacggtga aggatttcag
     9841 gcattttcgc aacgtgtgga aagatttatc gcaaggctta gtgaatatca gcactatcag
     9901 aatattttaa tcgtcagcca tcagggcgta ctgagtctgt taattgctcg tctaattggc
     9961 atgcctgccg aatctatgtg gcattttcgc gttgaccagg ggtgttggag cgccattgat
    10021 atcaaccaaa aattcgcgac gctacgcgtc ctcaatagcc gtgccatcgg ggtcgagaat
    10081 gcatgacttt tctgtttttt tacgggtaag ccgcaacgac cattgacagc cccgggcagg
    10141 ctgatattct ccgcagccag actttttccg ccagacacga ctttgtagaa attgttttac
    10201 aaaaatggcg atgcaatctg cggcgcgggg tgggatgata gcccactttc gaaagccgat
    10261 tcggcgacaa ttgtcccgaa atcgcctctg gttcaggtat actgacagac catttttatc
    10321 tatttgattc acccaggggg aaaacttgca gggtaaagca ctccaggatt ttgttatcga
    10381 caaaattgat gacctcaaag gtcaggacat catcgcctta gacgttcagg gcaaatccag
    10441 catcaccgac tgcatgatca tctgtacggg tacgtccagc cgtcatgtta tgtccattgc
    10501 tgaccacgtt gtgcaggagt ctcgcgcagc gggcctgtta ccgctcggcg tagaaggtga
    10561 aaacagcgcc gactggattg tcgtggattt gggcgatgtg attgtccatg tcatgcagga
    10621 agagagccgt cgcctgtatg aactggaaaa actctggagt taatgcgtga agctgcaact
    10681 tgtcgccgtg ggaacgaaaa tgccggactg ggtacaaacc ggttttaccg agtacctgcg
    10741 tcgttttccg aaagatatgc ccttcgagct gattgaaatt ccggccggaa aacgcggcaa
    10801 gaatgcggac atcaagcgca tactcgacaa agagggtgag cagatgttgg cggccgcagg
    10861 caaaaaccgc attgtcaccc tcgatattcc aggcaagccc tgggatacac cgcagttagc
    10921 cgctgagctg gaacgctgga agctggatgg tcgcgacgtc agtctactga ttggtgggcc
    10981 tgaagggttg tcgcctgcct gtaaagcggc ggcggagcag agctggtcgc tgtcggcgct
    11041 taccctcccc catccgctgg ttcgcgtgct ggtcgcagag agtctgtacc gggcgtggag
    11101 catcaccacc aaccatcctt atcaccgtga gtgataaggg agctttgagt agaaaacgca
    11161 gcggatgaaa ctacagaact cttttcgcga ctatacggca gagtccgcgc tgtttgtgcg
    11221 ccgggcgctg gtcgcctttt tggggatttt gctgctgacc ggcgtgctta tcgccaacct
    11281 gtataatctg caaattgttc gctttaccga ctaccagact cgctctaatg aaaaccgcat
    11341 taagctggtg cctatcgcgc ccagccgcgg cattatctac gaccgtaatg gtatccctct
    11401 ggccctcaac cgcactatct accagataga aatgatgcca gagaaagtcg ataacgtgca
    11461 gcaaacgctg gacgctttgc gcagcgtggt tgatctgacc gatgacgata ttgctgcatt
    11521 ccgaaaagag cgcgcacgtt cacaccgttt cacctctatt ccggtgaaaa ctaacctgac
    11581 cgaagtacaa gtagctcgct ttgccgtcaa tcagtaccgt tttccgggtg tcgaagttaa
    11641 aggctataaa cgtcgttact atccttacgc ttcggcgttg acccacgtca tcggctatgt
    11701 gtcgaaaatc aacgataaag acgtcgaacg cctgaataat gacggcaaac tggccaacta
    11761 tgcggcaacg catgatatcg gtaagctggg cattgagcgt tactatgaag acgtgctgca
    11821 cggtcagacc ggttatgaag aggttgaagt taacaaccgt gggcgtgtta tccgccagtt
    11881 aaaagaagta ccaccgcaag ccggacacga tatttacctg acgctggatc tcaaactcca
    11941 gcaatatatt gaaacgctgc tggcgggtag ccgcgcagct gtggttgtca ccgatccgcg
    12001 tacaggtggg gtgctggcgc tggtttccac gcctagttat gacccaaacc tgtttgttga
    12061 cggtatctcc agcaaagatt attccgcctt gttgaacgac ccgaatacac cgctggtgaa
    12121 ccgcgccaca cagggggttt atcctcccgc gtctacagtt aaaccctatg tggcggtttc
    12181 ggcattgagc gccggggtga tcacgcgcaa tacgacgctg tttgacccag gctggtggca
    12241 actgccaggt tcggaaaaac gttatcgtga ctggaaaaaa tggggccacg ggcgtctgaa
    12301 tgtcacaaga tcgctggaag aatctgcgga taccttcttc tatcaggtgg cctacgatat
    12361 ggggatcgat cgcctctccg aatggatggg taaattcggt tatggtcatt acaccggtat
    12421 cgacctggcg gaagaacgtt ccggcaacat gcctacccgc gaatggaaac agaaacgctt
    12481 taaaaaaccg tggtatcagg gtgacaccat tccggttggt atcggtcagg gttactggac
    12541 agcgacccca atccagatga gtaaggcact gatgatcctg attaatgacg gtatcgtgaa
    12601 ggttcctcat ttgctgatga gcaccgccga agacggcaaa caggtgccat gggtacagcc
    12661 gcatgaaccg cccgtcggcg atattcattc cggttactgg gagctggcga aagacggtat
    12721 gtacggtgtt gctaaccgcc ctaacggtac ggcgcataaa tactttgcca gcgcaccgta
    12781 caaaattgcg gcgaaatccg gtaccgctca ggtcttcggt ctgaaagcga acgaaaccta
    12841 taatgcgcac aaaattgccg agcgtttacg tgaccacaaa ctgatgaccg cctttgcgcc
    12901 atacaacaat ccgcaagtgg ctgtcgccat gattctggag aacggtggtg cgggtccggc
    12961 ggttggtaca ctgatgcgcc agatcctcga ccacattatg ctgggtgata acaacaccga
    13021 tctgcctgcg gaaaatccag cggttgccgc agcggaggac cattaatcat gacggataat
    13081 ccgaataaaa aaacattctg ggataaagtc catctcgatc ccacaatgct gctgatctta
    13141 ctggcattgc tggtttacag cgccctggtt atctggagcg ccagcggtca ggatattggc
    13201 atgatggagc gtaaaatcgg ccaaatcgcg atgggtctgg tcatcatggt ggtgatggcg
    13261 caaattcctc cacgcgttta tgaaggctgg gccccctatc tctatatcat ctgtattatt
    13321 ttgctggtgg cggtagatgc tttcggtgcc atctctaaag gtgctcaacg ctggctggac
    13381 ctcggtattg ttcgttttca gccgtcggaa attgccaaaa tagccgtacc actgatggtt
    13441 gcgcgcttta tcaaccgcga cgtttgccca ccatcgttga agaacactgg catcgcgctg
    13501 gtgctgatat ttatgcccac gctgctggtg gctgcacagc ctgacctggg aacatcaatc
    13561 ctcgttgcgc tttccggtct gtttgtactg ttcctctctg gccttagctg gcgtctgatt
    13621 ggcgtcgcag tagtgctggt ggcggcgttc attccgattc tgtggttctt cctgatgcat
    13681 gattaccagc gccagcgcgt aatgatgctc ctggacccgg aatcagaccc actcggcgcg
    13741 ggctatcaca ttattcagtc taaaattgct attggctccg gcggattacg cggcaaaggc
    13801 tggctgcacg gcactcagtc acagcttgaa tttctccccg aacgccatac cgactttatc
    13861 ttcgcggtac tggcggaaga gctgggatta gtgggcattc tgattctgct cgctctctac
    13921 attctgctga tcatgcgcgg gctgtggata gccgccagag cgcaaaccac ctttggtcgc
    13981 gtcatggctg gtggcttaat gctgatatta ttcgtttatg tcttcgtaaa tattggtatg
    14041 gtaagcggta ttctgccggt tgtaggggtt ccgctcccac tggtcagtta tggaggatcg
    14101 gcgctaattg tgctgatggc tgggttcggg attgtaatgt caatccacac ccacaggaaa
    14161 atgttgtcga aaagcgtgta agaggtgcgc aatgcgtaag cagtggctcg ggatctgcat
    14221 cgcggcagga atgctcgcgg catgtacaag cgatgatggt cagcaacaga cggtaagtgt
    14281 accgcagcct gcggtatgta acggccccat agttgaaatt agcggggcgg atccgcgttt
    14341 cgaaccactg aacgcgacgg caaatcagga ttaccagcgc gacggtaaaa gctacaaaat
    14401 cgtgcaggat ccgtctcgat ttagccaggc gggactggcg gcaatctatg atgccgaacc
    14461 aggcagtaac ctgacggcct ctggcgaagc tttcgatccg acacagctga cggcggccca
    14521 tccaacgctt ccgatcccca gctacgccag aatcactaac ctggctaacg ggcgaatgat
    14581 cgtggtgcgc attaatgatc gcggccctta cggcaacgac cgcgttattt cgctttctcg
    14641 cgcggcagct gaccgtctta acacgtcaaa caacaccaaa gttcgtatcg atccgattat
    14701 tgtcgcccag gatggttcgc tttctggtcc tggtatggct tgtaccacag tcgccaaaca
    14761 gacttacgcc ctgccagcac ctcccgattt aagcggtggc gcgggaacaa gttcagtgtc
    14821 tggcccgcag ggtgacattc ttccggtcag taattcgacg ctaaaaagcg aagatccgac
    14881 cggcgcgccg gtaaccagta gcggtttcct cggcgctcca acgaccttag cgcctggtgt
    14941 actggaaggc agcgaaccga cgcctgcgcc acagcccgtt gttacagctc cgtcgacaac
    15001 gcctgcaacc tcgcctgcaa tggtgacacc gcaagccgcc tcgcaaagcg ccagcggcaa
    15061 ctttatggtg caagtcgggg ccgttagcga tcaggctcgt gcgcaacagt accaacagca
    15121 actgggacag aagttcggcg tccacggtcg cgtaactcaa aatggcgcgg tctggcggat
    15181 ccagcttggc ccattcgcca gcaaagccga agccagtacc ttgcagcaac gtttgcaaac
    15241 cgaagcccaa ttacagtcat ttattactac cgcgcagtag cgtaaagcag gcatctgatg
    15301 tgtcaatttc tgtaaatgac gttaacaaag tcacgcggaa agtcagatgc ctgccggtag
    15361 tgcatttgct atagtagggc acttttttaa ttccatcacg gatgtcgtag ttcagaccat
    15421 gaataccatt ttttccgctc gtatcatgaa gcgcctggcg ctcaccacgg ctctttgcac
    15481 agcctttatc tctgctgcac atgccgatga cctgaatatc aaaactatga tcccgggtgt
    15541 accgcagatc gatgcggagt cctacatcct gattgactat aactccggca aagtgctcgc
    15601 cgaacagaac gcagatgtcc gccgcgatcc tgccagcctg accaaaatga tgaccagtta
    15661 cgttatcggc caggcaatga aagccggtaa atttaaagaa actgatttag tcactatcgg
    15721 caacgacgca tgggccaccg gtaacccggt gtttaaaggt tcttcgctga tgttcctcaa
    15781 accgggcatg caggttccgg tttctcagct gatccgcggt attaacctgc aatcgggtaa
    15841 cgatgcttgt gtcgccatgg ccgattttgc cgctggtagc caggacgctt ttgttggctt
    15901 gatgaacagc tacgttaacg ccctgggcct gaaaaacacc cacttccaga cggtacatgg
    15961 tctggatgct gatggtcagt acagctccgc gcgcgatatg gcgctgatcg gccaggcgtt
    16021 gatccgtgac gtaccgaatg aatactcgat ctataaagaa aaagaattta cgtttaacgg
    16081 tattcgccag ctgaaccgta acggcctgtt atgggataac agcctgaatg tcgacggaat
    16141 caaaaccgga cacactgaca aagcaggtta caaccttgtt gcttctgcga ctgaaggcca
    16201 gatgcgcttg atctctgcgg tgatgggcgg acgtactttt aaaggccgtg aagccgaaag
    16261 taaaaaactg ttaacctggg gcttccgttt cttcgaaacc gttaacccac tgaaagtagg
    16321 taaagagttc gcctctgaac cggtttggtt tggtgattct gatcgcgctt cgttaggggt
    16381 tgataaagac gtgtacctga ccattccgcg tggtcgcatg aaagatctga aagccagcta
    16441 tgtgctgaac agcagtgaat tgcatgcgcc gctgcaaaag aatcaggtcg tcggaactat
    16501 caacttccag cttgatggca aaacgatcga gcaacgcccg ctggttgtgt tgcaagaaat
    16561 cccggaaggt aacttcttcg gcaaaatcat tgattacatt aaattaatgt tccatcactg
    16621 gtttggttaa aaattgaaca cttgaaagtg taatttccgt ccccatatac taagcatcag
    16681 taaaaaaact cccgccttct ggcgggagtt gctatttaat tacgttacgc cggagctgac
    16741 atgaaaacca aacttaacga actgcttgaa ttccctactc cttttactta caaagttatg
    16801 gggcaggcgt tacctgagct ggttgatcag gtggttgaag tggtacagcg ccatgcgcca
    16861 ggtgactaca ccccaacggt aaaaccaagc agcaaaggca actaccactc ggtatctatc
    16921 actatcaacg ccactcatat cgagcaggtt gaaacactgt atgaagaact gggcaaaatc
    16981 gatattgtcc gcatggttct gtaactcgct tctccgttac ccggctcctg gtcgggtaac
    17041 tcccccctcc tcgctgtgat atacttttcc cccactttta ctcattctcc acggagatgc
    17101 cgttttgtat caggataaaa ttcttgtccg ccagctcggt cttcagcctt acgagccaat
    17161 ctcccaagct atgcatgaat tcaccgatac ccgcgatgat agtacccttg atgaaatctg
    17221 gctggtcgag cactatccgg tattcaccca agggcaggca ggaaaagcgg agcacatttt
    17281 aatgccgggt gatattccgg tgatccagag cgatcgcggt gggcaggtga cttatcacgg
    17341 gccggggcaa caggtgatgt atgtgttgct taacctgaaa cgccgtaaac tcggtgtgcg
    17401 tgaactggtg accttgcttg aacaaacagt ggtgaatacc ctggctgaac tgggtataga
    17461 agcgcatcct cgggctgacg cgccaggtgt ctatgtcggg gaaaagaaaa tttgctcact
    17521 gggtttacgt attcgacgcg gttgttcatt ccacggtctg gcattaaacg tcaatatgga
    17581 tctttcacca tttttacgta ttaatccttg tgggtatgcc ggaatggaaa tggctaaaat
    17641 atcacaattg caacccgaag cgacgactaa taatattgct ccacgtttac tgcaaaatat
    17701 tttagcgcta ctaaacaatc ctgacttcga atatattacc gcttaattcc atacatcaat
    17761 ggcccaattt acactgggtc attactcata atacctgcta atgctttaca tctccttcgc
    17821 gtttgtatat tttctttatc acttgcagaa atgcgatgct acgccttgct ccgtaactca
    17881 ccccattgat taacctattg aaatagttga ttatggtgag caaaagcgtt ccgttccaat
    17941 tagacataag attattgtaa ttttttaaat attatttttc catgaataaa attggagtaa
    18001 gtgcgtggat agtaataatc aaattgaacc ctgtttaagc agaaagtcgt cagaaggtaa
    18061 accacaaata tttacaacct tacgaaatat tgatcttaac cttctgacta tttttgaagc
    18121 tgtatatgta cataaaggga tcgttaatgc agcgaaagtg cttaatctga ccccctcggc
    18181 aatcagtcag tctattcaga aactgcgcgt tatattccct gacccattgt ttattcgcaa
    18241 aggccagggt gtcactccta ccgcatttgc gatgcatcta catgagtata tcagtcaggg
    18301 ccttgagtcc attcttggcg cactggatat cgaaggaagc tatgataagc aacgaacgat
    18361 aactattgcg accactccct cggtcggagc cctggtcctt cctgtcatct atcgggcgat
    18421 taaaactcac tatccgcagc ttttactgcg caacccgccc atcagcgacg cggaaaacca
    18481 actcagtcag tttcaaaccg atctcatcat cgataacatg ttttgcacca atcgtacggt
    18541 gcaacatcat gttctgttca ccgacaatat ggtgttaatt tgccgcgagg gaaatccact
    18601 actctcttta gaagatgaca gagagactat cgacaacgct gcgcatgtac tcctgttacc
    18661 ggaagggcaa aatttcagcg gtctgcggca gagagttcaa gagatgtttc cggaccggca
    18721 aatcaatttc accagctaca acattttgac aatcgctgca ctggttgcca acagtgacat
    18781 gttagcgatt attcccagcc gtttttataa cctgtttagt cgttgctggc cgctggaaaa
    18841 actgcctttt ccgtccttaa atgaggagca aatagatttc tctatccact acaacaaatt
    18901 cagcctgcgt gctcccatcc tgcacggggt aatagatgtt atccgtaatg cattttgaaa
    18961 aagtattgtt acagccagat cacagcgcag aacgataaaa tggcacaatc caacaacaat
    19021 tttacatttt cgcgaccgct ttggctgctt tcaggtccgt ttcaatgata tactgccagt
    19081 cgttaattca aaaatagttg ataattacaa caatctattg aattgaaacg ctttccttcg
    19141 taattcgcaa ctggaacacg cacgctatga gtaaacccat tgtgatggaa cgcggtgtta
    19201 aataccgcga tgccgataag atggccctta tcccggttaa aaacgtggca acagagcgcg
    19261 aagccctgct gcgcaagccg gaatggatga aaatcaagct tccagcggac tctacacgta
    19321 tccagggcat caaagccgca atgcgcaaaa atggcctgca ttctgtctgc gaggaagcct
    19381 cctgccctaa cctggcggaa tgcttcaacc acggcacagc aacgtttatg atcctcggcg
    19441 ctatttgtac ccgccgttgt ccgttctgtg acgttgccca cggtcgcccg gtagctcctg
    19501 atgccaatga accagtgaaa ctggcgcaga ccattgccga tatggcgctg cgttatgtgg
    19561 ttatcacctc cgttgaccgt gatgacctgc gcgatggcgg tgcccagcac tttgcggatt
    19621 gcattactgc cattcgggaa aaaagcccgc aaatcaaaat tgaaactctg gtgccggatt
    19681 tccgtggccg tatggatcgt gctctggata ttctgactgc aacgccacca gatgtgttca
    19741 accataacct ggaaaacgta ccgcgtattt accgtcaggt acggcctggt gcagattaca
    19801 actggtcgct gaagctgctg gaacgcttta aagaagcgca tccggaaatc ccgaccaagt
    19861 ctggtctgat ggtgggactg ggtgaaacca atgaagaaat tattgaggta atgcgcgacc
    19921 tgcgccgtca tggtgtgacg atgttaacgc tggggcaata tttgcagcca agccgccatc
    19981 acctgccggt tcaacgttac gttagcccgg atgagttcga cgaaatgaaa gccgaagcgc
    20041 tggcgatggg ctttacccat gctgcatgcg gtccgtttgt ccgctcttct taccacgccg
    20101 atttgcaggc gaaagggatg gaagttaagt aattctgtaa taattactta catctgtctg
    20161 ataaaaaacc cgctttttga agcgggtttt ttgtatcgaa caagatattg agggagcgtc
    20221 ctgctcgcca cgtcactctt tatgagagag cttttcagcc tgaagatcaa cgtctgcgcc
    20281 ttttttcgcc gcagcatcgt catcattcat cgccttcttg aaccctttaa tggccgctcc
    20341 aaggtctccg cccagcgtac gtaacttctt agtcccaaac agcagaacga ccagcgccgc
    20401 aactaccagc agtttggtaa tactaatctc acccatagat accttcttga catataaccg
    20461 gcaacagaac gcgccttaaa aaccagagaa tattaacggt cattcgacgc gcgcacacaa
    20521 tagcaatctg taacaaggtg aatcaagcgc cgagtaaaaa aacatcataa taattgcggc
    20581 ggcgcaaagc gacggttgtt taagacgggc agttgcgcgc gcacctgacg cacacgttcg
    20641 ggcgtcactt ccgccataat gagtgcaggc atttctgacg ctgccgcaat ggtgacgcca
    20701 aagggatcta taatccggct ttgcccgata tttttgttcc cgcactcccc cgccgccacc
    20761 atataacagg tggtatccag cgcacgagcg gcaagcaacg ttgaccagtg atgctctttg
    20821 agcggcccgc gaacccaggc ggcaggaagt accaggattt cagctccctg taatgcctgt
    20881 gccagcgcca gctctggaaa gcgtaagtca tagcaggtca tcagaccgac cttcatccct
    20941 tccacctcca gtaacggaac gatttcatta ccagcatcaa catggcgtga ttcctgaatg
    21001 gcaaatgcat catagagatg cagtttggca taacgggcga cgatgtttcc tgcctgtagc
    21061 gccaccagca tattccatgc gcgccccggc gttgaaggaa catgaatcgt cagaattgtc
    21121 gtcatcatgt tacgtttact ttctcgccgt aaacgtccga ggaattcacc ttccagcagc
    21181 tgtgctgatt taaccgatag atctgcatca tgatcatcgc gcgccagcaa tgcttccggc
    21241 aggacaaaca gcgatacgtc gttttccgcc gcctgcgcca tcaacgaggc acaaatctca
    21301 gcgttctttt cccacacaga tgtaacagca aactgtcctg cagcaaccag cattgccacc
    21361 tcctgtcaga gtcgtaaaat tcgccaacga cgattattcg gcgttacact tatcactcat
    21421 acaaatcaaa tagcaggatt ttgcagtgtt acaacttctt ttagcagttt ttattggcgg
    21481 tggtacggga agcgtggcga gatggctgtt aagtatgcga tttaacccac tgcatcaggc
    21541 gattccgttg gggacgctgg cagcaaacct gattggggca ttcatcatag gaatgggatt
    21601 cgcctggttc agcaggatga cgaacattga tcccgtgtgg aaagtattaa tcaccaccgg
    21661 attttgtggc ggtctaacaa ccttctcaac attttcggca gaagtggtgt ttttgttaca
    21721 agagggccgc tttggctggg cgttactgaa cgttttcgtc aaccttctgg ggtcttttgc
    21781 catgaccgca ctggctttct ggctgttttc ggcctcaacc gcacactaaa gaccaaaaaa
    21841 aacccgctga ttaagcgggt tttgaattct tgctgacgta tcttacagag cgattacgtt
    21901 tgcagcagaa gggcctttgg caccgttagt gatttcgaac tctacgcgct gaccttcagc
    21961 aagagtttta aaaccattag tctggattgc agagaagtgt acgaacacgt ctttgctgcc
    22021 gtcttccgga gtaatgaaac cgaatccttt ggactcatta aaccacttaa cgttaccttt
    22081 aatcttagac atcaaaatta cctttacatg aaaaatagac acaaatgctg tgtcggttac
    22141 cagtacacca attgtggtac gctttgtcca gtgcaacttt actaaaaagt gataaaagtc
    22201 gcgttatttt tgatgttact tattttagtt attgatttta aagaagttac taaaacttca
    22261 tttgtctcaa aactgaaagc gcatccaggc aaagtacaca ttgccattgt tgtaggtacc
    22321 cggaatgtag gtcatctgaa aagtcactgg gccataacct acggaggcca atggcagtag
    22381 aaccgggaga gggatgtaat tccagttatc gcgtgccgtt acgccagcgg tgaatcccag
    22441 acctaaatga aaattttcat ccgccagcgg tcgccaggta ctttcccatc cgtatccggc
    22501 aatcggttcc catttgttcc acgagtcctt aaatgccatg gcatacaggc catgccagtt
    22561 tcctttttca tcccaacgcg actgaccaaa accgccaccc cacggtcgct cgttatagcg
    22621 atcggttttt tctttgtcgt aagcgaaacg tgcatgccag gtgatggcag gaatatataa
    22681 atcataatgt tcaggctgtt gccaggcttg tgcaatattt tctctaaacg ttgtcatcca
    22741 ctcatctgcg ttagcaaaaa ctttaccaac gctgattaac tgaataaaaa caaaggaaaa
    22801 gatagcgaca tatttactca cgttcatttg tgaccataaa acatttatca aaaatctact
    22861 actagcatag caaagctaca attaacataa ccttaatagc cccaacataa agaataatct
    22921 gaatagctgc tctatttatt tccgcttgtt caagttaaat atttccgcgc atcgattaaa
    22981 gatgatgcgt cgttttctgc gatgggaata gtcaaaaaag aaaaaccaag
//
LOCUS       DAZKIV010000069        22664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZKIV010000069 DAZKIV010000000
VERSION     DAZKIV010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.069"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..306)
                     /locus_tag="ACHOT6_002592"
     CDS             complement(<1..306)
                     /locus_tag="ACHOT6_002592"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947785.1"
                     /translation="MEIKDVIALVGLIIPGITAAVGVYKLLNDVSWFLSKTTKFSHIL
                     ENYREYIDPSELEFMKAEIKREIKKSILGMVNLKLRPLILYIRTYSELDMPSWRWWFL
                     "
     gene            333..2396
                     /locus_tag="ACHOT6_002593"
     CDS             333..2396
                     /locus_tag="ACHOT6_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HFP9947786.1"
                     /translation="MSNILWGDLETYCEIPITNGTHAYAEGVEVMLFAWAINDGPVNV
                     WDITAGGGIPHGLYEAIADPETLLYFHNSHFDRTVLRYAMPRLAPPVERWRDTMVQAL
                     AHGLPGSLGELCEVLGVPQDKAKDKEGKALIQLFCKPRPKNSKLRRATSKTHPEEWRR
                     FVAYAGLDIEAMREVYKRLPKWNYQGTELALWHRDQQINDRGVCMDVELARAAIDAVD
                     QEQKRLAKRTQEMTDGEVQAATQRDALIKHIVESYGVELPDMQRSTLERRIADPDLPS
                     AVKELLAIRLQASTTSTSKYKALMKGVSHDGRLRGTLQFCGASRTGRWAGRLFQPQNL
                     PRPSLKQEQIDEGIEALKAGCADLLFDNIMELTSSALRGCIIAPTGKKLVVSDLSNIE
                     GRMLAWLAGEKWKLNAFREYDAGTGPDLYKLAYAKAFDIAPDDVDKHMSQIGKVMELG
                     LGYGGGVSAFITFALVYGLDLDELANAALPNIPRDVIREAKSWYDESVKRKSTYGLSE
                     RVFIACDSLKRLWRRAHPATCDFWYELERTVRTAIATPQKTLYCGYLKIRRDGAWLRI
                     QLPSGRAVCYPSPVIEQGNITYMGVNSYSRKWQRLKTYGGKLVENVTQEAARDVLAGN
                     MPLIEDAGYSIVLTVHDEVITESPDTEDFNDKALSALLSTNPEWAPDIPLNAGGFEAY
                     HYRKD"
     gene            2402..2617
                     /locus_tag="ACHOT6_002594"
     CDS             2402..2617
                     /locus_tag="ACHOT6_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001419980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9947787.1"
                     /translation="MPSKYRDNPLYYRAAREALRLEQFGEYDRAAKVWAKANRESHHE
                     LNQEWSERRSDFCLMQNMREKRKAVEE"
     gene            2614..2913
                     /locus_tag="ACHOT6_002595"
     CDS             2614..2913
                     /locus_tag="ACHOT6_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9947788.1"
                     /translation="MIVYVAGPMSGYEQFNRPAFHSAAKRLTDKGYVVLNPATLPNGL
                     TQAQYMDICLAMLRCADTIYMLKEWEYSAGARAENALAEKLELNVLFEEWDSDGL"
     gene            2903..3172
                     /locus_tag="ACHOT6_002596"
     CDS             2903..3172
                     /locus_tag="ACHOT6_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HFP9947789.1"
                     /translation="MAYERESLIEKHLVAEVKKAGGVAFKFVSPGHRSVPDRIVLLPG
                     SRIVFVECKSPGKPPRPDQLREHERLRKLGFTVVVLDSKNLEGIL"
     gene            3169..3345
                     /locus_tag="ACHOT6_002597"
     CDS             3169..3345
                     /locus_tag="ACHOT6_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9947790.1"
                     /translation="MSKRDDPQLRVRIPQDLKDALEKISRENDRTLTAEITRRLRKSL
                     EDESTIHSQDLATS"
     gene            complement(3314..3643)
                     /locus_tag="ACHOT6_002598"
     CDS             complement(3314..3643)
                     /locus_tag="ACHOT6_002598"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9947791.1"
                     /translation="MSKRDDPQLRVRIPELLKEALEKKARANKRTLTSEIVSRLESTI
                     LQDKLLHTSRGFEEAADEILILRDLLEKLKTTYHREYQAEWVYENKYELIEAMEKLKK
                     LLNPENE"
     gene            3760..3936
                     /locus_tag="ACHOT6_002599"
     CDS             3760..3936
                     /locus_tag="ACHOT6_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947792.1"
                     /translation="MAIQITQLHQADIFGYIADMLETARLLSSLEKGEQLAFELIYFA
                     QQAAREAANKPWDE"
     gene            3996..4802
                     /locus_tag="ACHOT6_002600"
     CDS             3996..4802
                     /locus_tag="ACHOT6_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001094884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFP9947793.1"
                     /translation="MNTLTLINDTKQPMMGSRKIAEVTGKQHKHVVRDILAMLEQLQI
                     YSPDLDDDDFKGFIIKRKEYKGRDVIDEIWLDENLSMTLVTGYDAKRRLALIEQWQAM
                     KIELEQPRPASLPVPAPAIQVNDNIVQFARVVAEATASATMKAIVEVMAPRQVQNVTL
                     STPGHETNWVEDLKQQVRESQPHSNGEYVPVSKAAWKTGLSDSTCRKLIGFARIPVRS
                     DTCVRGLLVKLPAIEQAAQKLLNESTPPKGNRKRWRHPQFGNFTYYTDLI"
     gene            4846..6240
                     /locus_tag="ACHOT6_002601"
     CDS             4846..6240
                     /locus_tag="ACHOT6_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HFP9947794.1"
                     /translation="MGQIFTPRPYQDLIINHQIETLRGNIWAGMGMGKTVATLTTLED
                     LFMAGAETRPALVLAPLRVAANTWPDEAMKWGHLRNIEVQPIVGNAKARSAALANSNA
                     SVFTINYDNLVWLVEELGERWPFGTVIPDESTRLKSFRLRGGGKRAAALGKVAHKYVR
                     RWINLTGTPAPNGLVDLWGQSWFVDQGQRLGRTYGAFTSRWFNSIQFPGQSWTKLEPF
                     AHSQGEIQRALADVTLSLDAADWFDIKDPIHNVIRVDMPPKARQQYREMEKEMFLELN
                     GEGIEAPNAAAKTLKCLQIASGAVYTDDTGSWSELHDTKLQALDSILTEAAGAPVLVA
                     YHWKHDLERLLKAFPRGRHLDQDPQTLRDWNSGKIPVLFAHPASAGHGLNMQDGGNIL
                     VFFSHWWDLEQYQQIIERIGPTRQIQAGHNRPVFIHHIIATDTMDEMVMERRNSKRTV
                     QDILLDAMKKRGIA"
     gene            6237..6437
                     /locus_tag="ACHOT6_002602"
     CDS             6237..6437
                     /locus_tag="ACHOT6_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9947795.1"
                     /translation="MSEKPDDLLTPDEVCQKLGITQKTLCEWNIKHRHRAILAPIRFS
                     AKVVRYERRNVDAFIQKCRSQY"
     gene            complement(6434..7831)
                     /locus_tag="ACHOT6_002603"
     CDS             complement(6434..7831)
                     /locus_tag="ACHOT6_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9947796.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARRSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELAIQLA
                     REKALGMADGGLRAESVHAALEMYRDDLKAKVARQKLSPDSFTTYGVRIDRIKATFGE
                     REVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWKFCAPTLCNGRNVAASLPD
                     DYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNAVRFMIITGVRPINVHNLR
                     WDYVHEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRRIIDEQKAWRDSVPECNRD
                     YVFLQPRDPMQPFSKRSLDKLVKTYSPDGAVKGIKHDGTVKGKDGAFNTMCRKFLKSN
                     VIALMKERGYSRSDRREISLLCLHHSSKSDDPMAEHYDFSDEILQEEIALKREAFEAH
                     ERSILAQVALLRRRG"
     gene            8046..8306
                     /gene="yfhL"
                     /locus_tag="ACHOT6_002604"
     CDS             8046..8306
                     /gene="yfhL"
                     /locus_tag="ACHOT6_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFP9947797.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(8500..8580)
                     /gene="shoB"
                     /locus_tag="ACHOT6_002605"
     CDS             complement(8500..8580)
                     /gene="shoB"
                     /locus_tag="ACHOT6_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFP9947798.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(9001..9381)
                     /gene="acpS"
                     /locus_tag="ACHOT6_002606"
     CDS             complement(9001..9381)
                     /gene="acpS"
                     /locus_tag="ACHOT6_002606"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFP9947799.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(9381..10112)
                     /gene="pdxJ"
                     /locus_tag="ACHOT6_002607"
     CDS             complement(9381..10112)
                     /gene="pdxJ"
                     /locus_tag="ACHOT6_002607"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFP9947800.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(10124..10852)
                     /gene="recO"
                     /locus_tag="ACHOT6_002608"
     CDS             complement(10124..10852)
                     /gene="recO"
                     /locus_tag="ACHOT6_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFP9947801.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(10864..11769)
                     /gene="era"
                     /locus_tag="ACHOT6_002609"
     CDS             complement(10864..11769)
                     /gene="era"
                     /locus_tag="ACHOT6_002609"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFP9947802.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPVSAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(11766..12446)
                     /gene="rnc"
                     /locus_tag="ACHOT6_002610"
     CDS             complement(11766..12446)
                     /gene="rnc"
                     /locus_tag="ACHOT6_002610"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFP9947803.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(12719..13693)
                     /gene="lepB"
                     /locus_tag="ACHOT6_002611"
     CDS             complement(12719..13693)
                     /gene="lepB"
                     /locus_tag="ACHOT6_002611"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFP9947804.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(13709..15508)
                     /gene="lepA"
                     /locus_tag="ACHOT6_002612"
     CDS             complement(13709..15508)
                     /gene="lepA"
                     /locus_tag="ACHOT6_002612"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFP9947805.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(15706..16185)
                     /gene="rseC"
                     /locus_tag="ACHOT6_002613"
     CDS             complement(15706..16185)
                     /gene="rseC"
                     /locus_tag="ACHOT6_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFP9947806.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(16182..17138)
                     /gene="rseB"
                     /locus_tag="ACHOT6_002614"
     CDS             complement(16182..17138)
                     /gene="rseB"
                     /locus_tag="ACHOT6_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFP9947807.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(17138..17788)
                     /gene="rseA"
                     /locus_tag="ACHOT6_002615"
     CDS             complement(17138..17788)
                     /gene="rseA"
                     /locus_tag="ACHOT6_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFP9947808.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(17821..18396)
                     /gene="rpoE"
                     /locus_tag="ACHOT6_002616"
     CDS             complement(17821..18396)
                     /gene="rpoE"
                     /locus_tag="ACHOT6_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFP9947809.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(18393..18548)
                     /gene="rseD"
                     /locus_tag="ACHOT6_002617"
     CDS             complement(18393..18548)
                     /gene="rseD"
                     /locus_tag="ACHOT6_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFP9947810.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            18804..20426
                     /gene="nadB"
                     /locus_tag="ACHOT6_002618"
     CDS             18804..20426
                     /gene="nadB"
                     /locus_tag="ACHOT6_002618"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFP9947811.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(20411..21148)
                     /gene="trmN"
                     /locus_tag="ACHOT6_002619"
     CDS             complement(20411..21148)
                     /gene="trmN"
                     /locus_tag="ACHOT6_002619"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFP9947812.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            21280..22614
                     /gene="srmB"
                     /locus_tag="ACHOT6_002620"
     CDS             21280..22614
                     /gene="srmB"
                     /locus_tag="ACHOT6_002620"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFP9947813.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT         5707 a         5899 c         5626 g         5432 t
ORIGIN      
        1 taagaaccac caccgccaag atggcatatc taattcagaa tacgttctta tataaagaat
       61 taacggccta agtttcaaat taaccatacc caagatgctt tttttgattt ctctttttat
      121 ttctgccttc ataaactcta actcagaagg atcaatatac tcccgataat tttcaagaat
      181 atgtgagaat tttgttgttt tcgacaaaaa ccaactaaca tcattcaata atttataaac
      241 accgactgct gcggttatac ctgggattat gagtccgaca agtgcaataa catcttttat
      301 ttccatacaa ctaacccttc ggaataaata ccatgtccaa tatactatgg ggcgacctag
      361 aaacctattg cgaaatccct attacgaacg gcacccatgc gtatgccgaa ggcgttgaag
      421 tgatgctttt cgcatgggct atcaacgacg ggccagtaaa cgtgtgggat atcactgccg
      481 gtggtggtat tccacacggc ttatacgaag caatcgcaga ccctgaaacc ctgctttatt
      541 tccataactc tcacttcgac cgcaccgttc tgcgttatgc aatgccgcga ctggcaccgc
      601 cagtcgaacg ttggcgcgac acgatggtgc aggcgctggc gcacggtctc ccggggtctc
      661 tgggggaact ctgcgaagta ctaggcgtcc cgcaagacaa agcgaaggac aaagaaggta
      721 aagcgctgat ccagctgttc tgtaagccac gcccgaaaaa cagcaaactg cgtcgggcca
      781 ccagtaaaac ccacccggaa gaatggcggc gctttgttgc ttacgccgga ctggatatcg
      841 aggcaatgcg cgaagtctat aaacgtctgc cgaagtggaa ttatcagggg acagagctgg
      901 cgctctggca tcgtgatcag cagatcaatg accggggcgt ctgcatggac gtggaactcg
      961 cacgcgctgc gatcgacgcg gtagaccaag aacaaaagcg cctggcaaag cgtacacagg
     1021 aaatgactga tggcgaagtg caggcagcca cacaacgaga cgcgttgatt aagcacatcg
     1081 ttgaatccta cggtgtggag ctaccagaca tgcaacgcag taccctagaa cgtcgtatag
     1141 ccgaccccga tttaccatct gccgtgaaag aactgctggc tatccgcctg caggccagta
     1201 ctaccagcac cagtaaatac aaggcactga tgaaaggcgt aagccacgac ggacgcttac
     1261 gcggtacact acagttctgc ggggcgtcac gtaccggtcg ttgggccgga cggctattcc
     1321 agccccagaa ccttccccgc ccttcactaa aacaggaaca aatagacgaa ggcatcgaag
     1381 cactgaaagc cggatgtgca gacctgctgt ttgataatat catggaacta accagttcag
     1441 cgttacgtgg ctgcattatc gcgccaacag gcaaaaagct ggtggtaagt gacttgtcga
     1501 acattgaagg ccgtatgctg gcatggctgg cgggagaaaa atggaaactg aatgcattca
     1561 gagagtacga cgccggaacg ggtccggact tatataaact ggcgtatgca aaagctttcg
     1621 atattgcacc agatgatgtt gataaacaca tgagtcagat cggtaaagtc atggaactcg
     1681 gtctgggtta tggtggtggt gtgtcggcct tcatcacttt tgcactggtt tacggtctcg
     1741 atctcgacga gctggcgaac gccgcactac caaacattcc ccgcgatgtt atccgcgagg
     1801 cgaaaagctg gtacgacgaa tcggttaaac gcaagtcaac ctacgggctt tctgaacggg
     1861 tatttatcgc ctgcgactca cttaaacgtc tctggcgcag ggcgcacccg gcgacctgcg
     1921 atttctggta cgagctggag cgcactgtcc gcacagcaat cgccacaccg caaaaaacat
     1981 tgtattgcgg ttatcttaaa atccgccgcg atggcgcgtg gctgcgcata cagctaccat
     2041 ccggacgcgc tgtatgctac ccgtctccgg ttatcgaaca agggaatatc acctacatgg
     2101 gtgttaactc ttattcgcgt aaatggcaac gactcaaaac ctacggcgga aagctggtgg
     2161 agaacgtcac ccaggaggcc gcccgcgacg ttctggccgg aaacatgccg cttatcgaag
     2221 atgccggtta cagcattgtg ctgacggtac acgacgaggt gatcaccgaa tcacctgaca
     2281 cagaagattt caacgataaa gcgctttccg cgcttctctc cactaacccc gaatgggcgc
     2341 ccgatatccc actgaacgct ggcggttttg aggcgtacca ctaccgtaag gattaactcc
     2401 tatgccatct aaataccggg ataatccact ttattacagg gctgcaaggg aggctttgcg
     2461 acttgaacaa ttcggcgaat atgaccgggc agcgaaggtc tgggcaaaag ccaaccgcga
     2521 atcacatcac gaactgaatc aggaatggag tgaacgcaga tctgattttt gcctgatgca
     2581 gaatatgcgt gaaaagcgta aggcggtcga agaatgatcg tttacgttgc cgggccgatg
     2641 agcggttacg agcaatttaa ccgcccggct tttcattccg cagcaaagag gctaacggac
     2701 aagggatatg ttgtgcttaa ccccgcaact ttacccaatg gtttgacaca ggctcagtat
     2761 atggacatct gcctcgcaat gcttcgctgt gccgatacga tttatatgct caaagagtgg
     2821 gagtactctg ctggcgcacg tgcagaaaac gcgttggcag agaaactgga attgaatgtg
     2881 ctttttgaag agtgggattc agatggccta tgaacgtgaa agccttatcg aaaagcacct
     2941 cgtcgctgaa gtgaaaaaag ctggcggagt ggcctttaag tttgtatctc ccggtcaccg
     3001 ctcggtaccg gatcgcattg tcctgctacc cggtagtcgt atcgttttcg ttgaatgtaa
     3061 atcacccggc aagccaccac ggcctgacca attgcgcgaa catgaacgtc tgcggaaact
     3121 gggctttacc gtagtggtgc tggatagcaa aaatctggag gggatattgt gagtaaacgt
     3181 gacgacccac aattgcgggt acgcatcccg caagacttga aagacgcact ggaaaaaatt
     3241 tccagagaaa acgacagaac actaaccgcc gaaatcaccc ggcggttacg taaaagttta
     3301 gaggatgaat ccactattca ttctcaggat ttagcaactt cttaagtttt tccatcgctt
     3361 caataagctc gtatttgttt tcataaaccc attcggcctg atactctcga tggtacgttg
     3421 ttttaagttt ctcgagaaga tccctgagta tgagaatctc atcagcagct tcctcaaacc
     3481 ccctagatgt atgaagtaat ttgtcctgaa gaatagtaga ttccaaccgg ctaacgatct
     3541 cagaggtcag tgttctctta ttagcccgtg ctttcttctc caaagcctct tttagcaatt
     3601 ctggtatgcg taccctcaac tgcgggtcgt ctcttttgct catatatgat cccttgaaag
     3661 tgtgaggaca acatgcctca caaacatgtt gacttcaagc ctcattgtga gtactaataa
     3721 ataaagtcct cacaatgagt acaaaacaag gctaaagcta tggctattca gatcacacag
     3781 ttacatcagg cagatatttt cgggtatatc gcagacatgc tggaaacagc gagacttctc
     3841 agttcgctgg agaaaggaga gcagttagct tttgaattga tttattttgc gcaacaggcc
     3901 gcaagggaag cagcaaacaa gccgtgggat gaataaacaa aacacgctga tagttgcagc
     3961 taccagcgca tcttttactg aatggagaat acagcatgaa tactttaaca ctgataaacg
     4021 ataccaagca accaatgatg ggcagccgga aaattgcaga agtcactggc aaacagcata
     4081 agcatgttgt acgggatatt ctggctatgc ttgaacagtt acaaatctat agtccagatt
     4141 tggatgatga tgattttaaa ggatttatta tcaagcgcaa agaatacaag ggacgtgatg
     4201 tcatcgacga gatttggctt gatgaaaatc tttccatgac tctcgtcacc ggttacgacg
     4261 caaaacgccg gctggcactc atcgaacagt ggcaggcgat gaagattgaa ttagagcaac
     4321 ctcgtccggc atcattaccg gtgccagctc ctgcaattca ggtaaacgac aacatcgttc
     4381 agtttgcccg tgtcgttgca gaagctacgg catccgccac gatgaaagca attgtcgaag
     4441 tgatggctcc ccggcaagtt caaaacgtaa cgctttcaac tccgggacat gaaaccaact
     4501 gggtagaaga cttaaaacaa caagtcagag agtcacaacc acacagtaac ggtgaatatg
     4561 tccccgtcag caaagcagca tggaaaacag gtttatctga ttcaacatgc cggaaattga
     4621 tcggcttcgc caggataccg gtacgttcag acacctgtgt tcggggattg ctcgttaagc
     4681 tcccagcgat cgaacaagca gctcaaaagc tgttaaacga atcgacaccg ccgaaaggta
     4741 accgcaagcg ctggagacat ccgcagtttg gcaacttcac ctactacacc gatctaatct
     4801 gacattattg cgcgggatta cccgcgccct actggctaaa acactatggg acaaattttc
     4861 accccccgcc cgtatcaaga cctgatcatc aatcaccaaa tcgaaaccct gcgcggcaac
     4921 atctgggcag ggatggggat gggtaaaacc gtagcaacac tcactacgct ggaagatctt
     4981 ttcatggcag gggcagaaac acgccccgcg ctggtcctcg cgccgctacg tgttgcagca
     5041 aacacctggc cggatgaagc aatgaagtgg gggcatttgc gcaatatcga ggtgcagccg
     5101 attgttggta acgccaaagc gcgctctgca gcgctggcga acagcaacgc gagcgttttc
     5161 accatcaact acgataacct tgtctggctg gttgaggaat tgggagaacg atggccgttc
     5221 ggtactgtca ttccagatga aagcacccgg ctaaaatcct tccggctgcg aggtggtggt
     5281 aagcgcgcgg cggcgctggg caaagtggcg cataagtatg tccggcgctg gataaatctc
     5341 accggtacgc cagcaccgaa cggcctggta gatttgtggg gacaatcgtg gtttgtggac
     5401 caagggcaac gtctcgggcg cacttacggc gcgtttacct cacgctggtt caactcgata
     5461 cagtttccgg gacagagctg gactaaactg gagccttttg ctcactcaca gggtgaaata
     5521 cagcgagcat tagccgatgt gaccctctcg ctggatgcgg ccgactggtt cgatatcaaa
     5581 gaccccatcc ataacgtaat ccgcgtggat atgccgccga aggcccgtca gcagtatcgt
     5641 gaaatggaaa aggaaatgtt cctcgagctg aatggcgaag gcatcgaagc accgaacgcc
     5701 gcggcaaaga cactgaagtg tctgcaaatc gccagtggcg cagtatacac agacgacacc
     5761 ggaagttggt cagaactgca tgacaccaaa ctacaggcgc tggacagcat actgaccgaa
     5821 gcagctggcg caccggtgct ggttgcttat cactggaaac acgatcttga acgcttgctt
     5881 aaagcattcc ctcgcggtcg tcacctcgac caggatccac agacactgcg cgactggaat
     5941 tccggaaaga ttcctgttct ctttgcacac ccagccagcg cgggccacgg cctgaacatg
     6001 caggacggcg ggaatatact ggtatttttc tcacactggt gggacctgga gcagtatcag
     6061 caaattattg aacgtatcgg ccccaccagg cagatacagg ccggacacaa tcgtccggta
     6121 tttattcacc acattattgc taccgacact atggatgaaa tggtgatgga gcggcgcaac
     6181 tcaaaacgaa cagtgcagga catcctgctc gatgccatga aaaagagagg tatagcatga
     6241 gcgagaaacc cgacgattta ctcaccccgg atgaagtatg ccaaaagtta ggtattacac
     6301 agaaaacgct atgtgagtgg aatattaagc atcgtcatcg ggctatcctg gcaccaattc
     6361 gtttcagtgc aaaagtagtt cgttatgagc gccgaaatgt cgacgctttt attcaaaaat
     6421 gtcgcagcca gtattaacct cgccgccgta gcaatgccac ctgcgcaagt atgctccgct
     6481 cgtgagcctc gaaagcttcg cgcttcaacg caatctcttc ctgtaaaatc tcatcagaaa
     6541 agtcgtaatg ttctgccatc gggtcatctg acttactgga gtggtgaagg caaaggaggc
     6601 tgatttccct tcggtctgat cgggaatagc ctctttcctt catcaaggca ataacattgc
     6661 tcttaaggaa tttacggcac atcgtattaa atgcaccgtc tttcccttta acagtcccat
     6721 catgttttat tccttttaca gccccgtccg ggctgtatgt tttcaccagc ttatccagtg
     6781 atcgttttga aaatggctgc attggatcac gtggctgcaa aaatacataa tccctgttgc
     6841 actcaggaac tgaatcacgc caggctttct gctcgtcgat aatccgccgg atctcaggcg
     6901 ttatcggcag gcggaaagcc ttttgtgttt tcatagcccc tcgcatgccg ataacccctt
     6961 ccggataaac aatttcacca gcctcctcgt gaacgtagtc ccagcgcagg ttatggacat
     7021 taatcgggcg aacgccggtg atgatcatga agcgaacagc attcttctgg tgtacagagg
     7081 tgcaggcggc aacattgagc cagagccggg cgattgactc aatatcggta aaaagccgtg
     7141 tgggggtggg tttctgtacg cgggaggaaa cataatcatc tggcagactg gcggcaacat
     7201 tgcggccgtt gcaaagagta ggtgcgcaga acttccagaa ccgacggagc tcggcaaaca
     7261 actctagggc gttattgttc gagcgagtgg cgatccactc gtccagtact tccaccagcc
     7321 gattgtatgt tacgtcgctg aacacctctc gctcgccgaa cgttgcttta atccggtcga
     7381 tacgcacccc gtaggttgtg aaactgtccg ggctcagctt ctgccgtgct actttagctt
     7441 tgaggtcatc ccggtacatt tccagcgctg catgtacgga ctctgcccgc agtccaccgt
     7501 cagccatacc tagtgctttt tcgcgcgcca gttgaatagc gagctccggc cactcgccaa
     7561 gctttttacc tttgaggccc atcttttttg gaaactcggc gtaaaatgta accttaccgg
     7621 ctttgctgaa atcgatacgg agatagttct ctttttcgta tttggaacgg cgagccacgc
     7681 cggaagcagc gaggatgatt ttggcagcag caacacagat tttcatgtgt gcgctggtat
     7741 aggggggttt acaggcgtcc catttttcag atgcggctaa aacatcgtca ttattggggc
     7801 tatccggatt atgtgttaca gtgcgcggca ttctcaatcc ttatctgcgt aggcgcagaa
     7861 aacaagctca cacatacaag tcttttctac gggacaaaat gcaatgtgtt gcggttttgt
     7921 gttgctggac tgagtttatc aaggttaaat acactggatc aacatacagt aagttaatga
     7981 cagtaaagca tacaaactcg atacaactta ctgagtttaa aatgatttaa cggtaatcca
     8041 ttgaaatgtc tttactaatt actaaacgct gtattaattg tgatatgtgt gaacccgaat
     8101 gcccgaatga ggcgatttca atgggagatc atatctacga gattaacagc gataagtgta
     8161 ccgaatgcgt agggcactac gagacaccaa cctgccagaa ggtgtgcccg atccccaata
     8221 ctattgtgaa agatccggcg catgtcgaga cagaagaaca gttgtgggat aaatttgtgc
     8281 tgatgcacca cgcggataaa atttaatatt ctactctgga agtagagtat taattatatt
     8341 actgggaagc cttaacgcca ttatatttat ttaattgatg acattagcat aatcattcac
     8401 taagttaatt tatatagtat ctgccaagac acttatttat agttattaaa ggcgcgtccg
     8461 attggttcac cggacgcacc ttaagtacgt ttccttgtgt tataagaaca gaaggatcag
     8521 ctgtaaaaca gcaatgatga ttttgatgac ccgtttaatc aggtatcggc aatcagtcat
     8581 tcgtttttcc ttaaacaagg aatgcagccg ttcagattag cccttacatc tccccaaaac
     8641 tgaacgtgcg agttattgag ggtgcatgct gcactccaca ccagagcttt gacgacacca
     8701 ctcgtttcaa tgggggaatt ctgtggcatg gtgtaaagca cagcaaaatc ttcaataacg
     8761 aagccaattt taatgtactt acgaattggc ggtcacgtta atctttccat caatattgct
     8821 ttcttcgtaa aggctcgagt ttttatgcta aagattgcaa gttgcttgta aaagataagt
     8881 acactgatcc ataatcgctg ttgttgaggg tgcatgctgc acaaaattaa agttaaaaag
     8941 taaaaccccc gttccttacc agttcggggg ttttactttt taaagagaac ggtattattt
     9001 ttaactttca ataattaccg tggcacaagc atagtgccgc tcatcggcaa gcgttacatg
     9061 catatttaca acgccgagct tttccgccag ttttaatgcc tcaccccata gccgtagccg
     9121 tggtttgccg agctcatcat tgaatacttc aaattgatta aacgccagac cattgcggat
     9181 cccggtgcca aacgcttttg ctgcggcttc tttcacagca aaacgcttcg ccagaaaacg
     9241 caccggctgg tggtgcgttt tccagattgc ccattcgtta tcgcttaata cgcggcgtgc
     9301 caggcgctca ccggatcggg cgatcactgc ttctatgcga gcgatctcta cgatatccgt
     9361 gcctaaacct aatattgcca ttagccacgc gcttccagca taagacgctt catttctgcc
     9421 accgcatctt tcagtccggt catcactgca cgaccaataa tggcatgacc gatattcagt
     9481 tcatgcatct cagggatggc ggcaatagct ttcacgttgt gataggtcag accgtgacca
     9541 gcgttaactt tcagaccgag actagcggca aaggtcgcgg ctttggcgat acgcgccagc
     9601 tcttgcgcct gttcggcgtc agttttggca tcagcatagc aaccggtgtg gatctcgata
     9661 aacggtgcgc caacctctgc cgcagctttg atctgctctt catcggcgtc gataaagaga
     9721 gaaacctgga tcccggcatc tgccagacgt ttgcaggcat cgcgcatttt gtcacgctgc
     9781 cctgcgacgt ccaggccgcc ttcggttgtt acttcctgac gcttttccgg taccagacag
     9841 caaaaatgtg gcttcgtctc aacggcgatc gccagcatct cttcggtcac cgccatctcc
     9901 agattcatgc gggtatccag tgtctgacgc aggatgcgca catcacggtc agtaatgtgg
     9961 cggcgatctt cacgtaaatg cacggtaatg ccgtccgctc ccgcctgctc agcaataaac
    10021 gccgcctgca ccggatccgg gtaagcagta ccgcgtgcgt tacgcagcgt tgcaatatgg
    10081 tcaatgttga cgcctaacag taattcagcc atgacaatcc tcatcattca taatgtgttt
    10141 tcaccgttcg cttaggcata aactgccgga acagttccct gctctttaat ggtttaccgc
    10201 caagatacgg cttaagcgcc atgcgggtaa agcgtttcgc ggcgcgtagt gtgtctgcgt
    10261 caggaaattc ccgcgcgttt aacgctttta actgccttcc ggtgaacgtt ttattgtcga
    10321 taacgacgct tgcgataaac cctttttctt cgcgataacg atacgtcatg gtgtcatcta
    10381 ccggctcgcc gctacccgca caatgggtaa aattgacgcc atagcccaga tgcccgagca
    10441 gtgccagttc aaagcggcgc agcgcgggtt ctggcgtacc agtggcacct gcaagagact
    10501 gaatgcagtg caagtaatcg aaaaagagtt cagagaagcg cgtctcgtat tccagtacgc
    10561 gggagagaag ttcgttgatg tacagaccgc tgtaaagcgt gataccgctt aatggcagcg
    10621 ccagcgaaac agcttcagca ctgcgcagcg ttttgacttc gccacgcccg ccgaaacgta
    10681 gcaagagagg ggtgaaaggc tgtaatgcac ctttcagagt agagcgttta gagcgtgcgc
    10741 ctttggcaac cagacgcacg cgacccgatt cctccgtgaa gacgtccagc atcaggctgg
    10801 tttcgctcca cgggcgacta tgcaggacaa atgcgcgctg ccagccttcc atcggagtga
    10861 ctcttaaaga tcgtcaacgt aaccgagact gcgcagtgcg cgttcgtcgt cggcccaacc
    10921 ggatttcact tttacccaca gctcaaggtg aacaggcgct tcgaacattt cctgcatgtc
    10981 tttacgcgct tcaatcccga tggttttgat cttggcccct ttgttgccaa taaccatctt
    11041 cttctgccct tcacgctcaa cgagaatcaa accgttgatg tcataaccac cgcgttcgtt
    11101 agagacaaaa cgttcgatct ccacggtcac ggagtacggc agttcagcgc cgaggaaacg
    11161 catcagtttt tcgcggatga tttcagacgc cataaaacgc tgtgagcgat cggtgatgta
    11221 atcttccggg aagtgatggg tcgcttcagg cagatgctta cgcacgattg ccgcaatagt
    11281 gtcaacattc aacccggttt cggcagagac cggcacgata tcgaggaagt tcatctggct
    11341 tgccaggaac tgtaagtgtg gcagcagatc ggctttttcc tgcacgttat ccactttgtt
    11401 caccgcgagg atcaccggag ctttgccatc gcgcagtttg ttgagcacca tttcatcgtc
    11461 cggcgtccag cgggtgcctt ctacgacaaa aatgaccagc tcaacgtcgc caatggagct
    11521 gctcgccgct ttgttcatca ggcggttaat ggcgcgtttt tcttccatat gcaggcccgg
    11581 ggtatcgacg tagatcgcct gatacgcgcc ttcagtatgg atccccacaa tgcggtgacg
    11641 agttgtttgc gccttgcggg aggtgatgga gatcttctgc cccagcagtt tattcaacaa
    11701 tgtggatttg ccaacattcg gacgtccgac gatagcaata aatccgcagt aacttttatc
    11761 gatgctcatt ccagctccag ttttttcaac gcctgttcgg cggcagcctg ctcagcctta
    11821 cgacggcttg aacctgtgcc taccaccggt tcactcaggc cgctgacctg gcagtggata
    11881 gtaaattcct gatcgtgcgc ttcgccacgt acctggacta ccagataagt cggcagcggc
    11941 agatggcgac cctgcaaata ttcttgcaag cgcgttttcg gatctttttg tttatcgcct
    12001 gggctaattt cgtccagacg ggtttgatac cagttaagga ttaatttctc gacggtttga
    12061 atatcactgt cgaggaatac gccaccaatt aatgcttcga cggtgtcggc gagaattgac
    12121 tcacgacgaa atccaccgct tttaagttca cctggcccta aacgtaagca ctcgcctaac
    12181 tcaaattcgc gcgccagttc tgccagcgta ttgccacgga ccagcgtggc gcgcatccgg
    12241 ctcatatcgc cttcatccac acgagggaaa cggtgataaa gcgcattggc gataacgtag
    12301 ctcagaatag agtcgcctaa aaattctaaa cgctcgttat gtttgctgct ggcactacga
    12361 tgagttaatg cctgctgcaa cagttcctga tgattaaaag tgtagcccag cttccgttga
    12421 agccgattaa ttacgatggg gttcatgcga taccaataaa taaatgcgtc aacaattcag
    12481 cacacgaaac agacctgata tacatggctc tgctaactgc ttcgctgcag tttcagttta
    12541 ccggtatatg gggaccaacg ctgtttcgtg tgccgtggca acctgaaggt gccaacctta
    12601 aacttcgggg gaatattcta tacacaacga cgggggatgt cgttagccac gggagattta
    12661 tctcataaat aattcacgtt gtcgccataa cggcgacaac gtgaacgaag atgggctatt
    12721 aatggatgcc gccaatgcga cttaagcgca cacctgtcgg ccattcgcct tcttgcttat
    12781 cgaaactcat ccagatagcc gttgcccgac cgaccagatt cgcttcaggc acaaaacccc
    12841 agtaacggct gtccgcgctg ttgtcgcggt tgtcgcccat catgaagtat tgtcccggcg
    12901 gaacaatcca ggttgccagt tgttgccctg gctgctggta atacatcccc acctggtcct
    12961 gcgcaatcgg cactgtcaga attcggtgcg tcacatcacc caatgtctct ttacgctcgg
    13021 aaagacgaat tccattttct ttggtttcgt ttttcggcac ttcaaagaat ccgctggtcg
    13081 cttccccacc attacggcgt gagaaggtct gaacgaaatc gctcggttcc acgttggagt
    13141 aggtgaccgg cagcgcgttt tcacatgcct ggccggaact gcatcccggt tgaatcgtca
    13201 gctctttcga gaccggatca taagtaactt tatcgcccgg taaacccacc gcgcgcttga
    13261 tgtaatcaag ctttggatct tccggatatt taaagaccac gatatctccg cgtttcggat
    13321 gaccggtttc gatcagcgtt ttctggtaga taggatcttt aatgccataa gcaaactttt
    13381 ctaccagaat aaaatcaccg attaacagag tcggcatcat cgaacctgac gggatctgga
    13441 acggttcata aataaacgaa cgcacaatca atacgatagc cagtaccgga aaaacagaag
    13501 caccggtttc cagccagcca ggcttcggcg caaccttttt caacgttgct ttatccagtg
    13561 agtcaccggc agccgcctgc gccgctgcct gacgttcccg ccgtttaggt gcgaaaaaga
    13621 atttatccac gcaccataaa atgcccgtca ccagtgtggc aatcaccaga atcagggcaa
    13681 acatattcgc catgccaact cctaagggtt atttgttgtc tttgccgacg tgcagaatgg
    13741 cgaggaacgc ttcctgcggc agctcgacgt taccgatctg cttcatgcgt ttcttacctt
    13801 ctttctgctt ctgcagcagc tttttcttac ggctgatatc gccgccataa catttagcca
    13861 gtacgttttt acgcagctgt ttcacggtgg atcgcgcaat aatgtgcgta ccaatcgctg
    13921 cctgaatggc gatatcaaac tgctggcgtg ggatcagatc tttcatcttc tccaccaact
    13981 cgcgaccgcg gttttgcgaa ttatcacggt gggtgatcag cgccaacgca tcaacacgtt
    14041 caccgttgat taatacgtct acacgtacca tgtcggacgc ctggaagcgt ttgaagttgt
    14101 aatccagaga cgcataacca cgcgaggtag atttcaggcg atcgaagaag tcgagcacca
    14161 cttccgccat cgggatctcg tacgtcagcg ccacctgatt accgtggtaa accatattgg
    14221 tctgcacgcc gcgtttttct acgcacagcg taataacgtt gccgagatat gcctgcggca
    14281 gcagcatgtg acattcggca atcggctcgc gcagttcgta gatgttattt accgctggca
    14341 gcttggatgg actgtcgacg taaataactt ctcttgacgt ggtttcaact tcatacacta
    14401 cggtcggcgc agtggtgatc agatccagat cgtattcacg ttccagacgt tcctggatga
    14461 tctccatgtg cagcaggccg aggaagccgc agcggaaacc aaagcccagc gcgctggagc
    14521 tttccggctc atagaacagt gaagcatcgt tcaggctgag tttacccagc gcgtcacgga
    14581 aggcttcata gtcgtcggaa cttaccggga acagaccggc gtatacctgc ggtttaactt
    14641 tcttaaagcc aggcagcgcc ttttctgccg gattacgcgc cagcgttaag gtatcgccga
    14701 ctggagcgcc gtggatatct ttaatcgcac ataccagcca gcctacttcg ccacatttca
    14761 gttcagtgcg gtcaacctgt ttcggcgtga agatgcccag acggtcggcg ttataggtct
    14821 gcccggtact catgactttc actttgtcgc ccttacgcag ggtgccgttt ttaatacgga
    14881 taagtgaaac aacgcccagg tagttgtcga accatgagtc gataattagt gcctgcaacg
    14941 ggccttccgg atcgccttcc ggcggcggaa tgtcgcgcac cagacgttcg agaacgtcct
    15001 gcacgccaac gccggttttc gctgaacagc gcaccgcgtc agtggcgtcg atgccgacga
    15061 tatcttcaat ttcttccgcc acgcgttcag gatcggctgc cggcaggtca atcttgttca
    15121 gtactggcac cacttcgaga tccatttcca tggcggtgta gcagtttgcc agggtttgcg
    15181 cttccacgcc ctgcccggcg tcgaccacca gcaatgcacc ttcacaggca gccagcgaac
    15241 gggaaacttc ataggagaag tctacgtggc ccggggtgtc gataaagtta agctgatagg
    15301 tttcgccgtc agacgctttg tagtccagcg tcacgctttg cgctttgatg gtaatgccac
    15361 gctcacgctc aagatccatg gaatcgagaa cctgcgcctc catttcacgg tcagacaggc
    15421 caccgcagat ctggataata cggtcagaca gcgtcgattt accgtggtca atgtgagcta
    15481 tgatcgaaaa gttacgtata ttcttcataa agtatgatta ttgtgcctta atgcccggtt
    15541 aaccaggctt ttagaagtcg ctgttctgag cttaacgtct gtattaatag aaacgccgca
    15601 ttctacacta caacattgag gcgaggaaat gttcataccg tatggattgt ggtatctgga
    15661 aacgtcctcg catttgttat gcaaaatgca acaaagccag tgaaatcact ggctcgcgtc
    15721 ttccgaagat gtttcaaatc gcacaaggcc aggcggcaat gccacgctta agatgatcgg
    15781 ttgccattct gcccgggcag caaacttacg cgagtagccg cgcgcaatca ggaacccgcc
    15841 aataccaccg aggatcgcac cacataatgc tgcgacgtca gaagcaaaga gtagctgaaa
    15901 tagcgaagcg ataaggaata atcccaccag cggcgacata taaaccagta atgcggagct
    15961 aagcaggctg ccttcggcga tccccaattc cactttttgc cccggcacta acggctcatc
    16021 acagggtacg acaatggtat gcgtggtttg cgggccaagt ttatttaaca cgcggctacc
    16081 gcaaccggcg cgtgaagcgc agctgctgca tgaagcttta acatcacaac tgaccagcgc
    16141 ctgcccgttt tgccaggaga cgacggtagc ccactctttg atcattgcgc tgccccgaac
    16201 ttaatattct cggcaatgcg tttcgccgtt tgcggcggca gttcaccgac aatggtgatt
    16261 tcggcgttat cacgtacgct tgtactgacg gttctgcgtc cggtgcgcaa catctgatcg
    16321 gtgctcgatg gcgtagcgcg gttaacgttt accgagaagc tgaataatcc gtcggaatag
    16381 agacgtgatt cgataggcat gttgtccatc gtcggtagcg gacgtcgact actggaaact
    16441 tcgctaaaac cctgtggcaa ccaggttggc gtccagctga atttagcttt ttcacctaca
    16501 ggaacagaaa gcaacggcgg caaatttgcc ttcgccagcg tctgcatact gctgctgata
    16561 tcctgattga cgttaaaagc aatcacgcga aattgttcca gcgtttcacc atcgcgatca
    16621 aggagatcaa cccgcatcgg taatttcgat tcggtgtcca tccacacgat gtagctgtag
    16681 cgtgtaccat ctcgggcaac cacgcgaatg acttcgcaaa gacgatcagc aatacgcgtg
    16741 cgcccgacgg agataaagtc gtagtaagga gaaaggcgtt tgaaatcggt atagataagc
    16801 gatggcagag aatcaacaat gtaatcgcca ttaagcgtga acggttcaag tcccggttca
    16861 aaatagctga tttcattgcc gcgctgtacc acttcccggc gcgggccatc catttgcaac
    16921 aattgtgcaa gaggacggtt atcgaggcgt gcatgtcgat aacgcagaga ctcaacaccc
    16981 tgtttattga tgctgatgaa tgacagctcg taattcagtg actgactggc caggttcatc
    17041 tgctgtaata acgccccgga cgcgggagtg gccgaggcgt tagcagagaa taacaggcta
    17101 cctgtcacta atgacatggc aaaccaaagt tgcttcatta ctgcgattgc gttcctaaag
    17161 tttgaattcc tggcacctgt acagcggctt gctgggtttg cgcctgctca aactgaagct
    17221 gttcagagtg gagtcggcgt tgcagttcgt aatcctgcaa cattgcatta atgcgacgac
    17281 gctgctcctg tacctgctgc tgttgaccat tgtttgcggt cgcttcagaa ggtactccca
    17341 ggcttaccgg gctggcttta cccatcatcg gcagtgtatt aaataccggc gtttcgggct
    17401 gctgggacgt ttcagattgt ccattatagt gctggacgcc aacgataact gcaagcgata
    17461 cgcatgctgc tacgcccatt tgggtaagct gtgccgccca cggacgtact ttctgccaga
    17521 atggcatttt ctgccattga tgcggcgcag gctgggcttc cgggatcaat gtcgccggtt
    17581 gacgtactgg ctcttcttca atggcggcca tcacgcgtga agagatatcg aaatggagca
    17641 cctcgggagt atcaccccgc attgaatcac ggattaagtg atagctttcc caggtttttt
    17701 gcatttctgg gttatgagcc agttcgttaa gcagctcact atccagcgtt tcgccatcca
    17761 ttaaagcgga aagttgttct ttctgcatgc ctaataccct tatccagtat cccgctatcg
    17821 tcaacgcctg ataagcggtt gaactttgtt atcaatagct tccctcgctc ggaagatacg
    17881 tgaacgcacc gtacctaccg gacaatccat gatagcggct atctcttcat agctcaggcc
    17941 atccagctcc cgcaaggtta ttgccatgcg caaatcttcc gggagggact caatagttcg
    18001 gaaaactatc tgtctcagtt cttctgacaa cattaagttc tcagggttcg aaatttcttt
    18061 caacgcgccg ccactttcga agttttcagc ttcaatggca tccacatcac tggaaggtgg
    18121 acgacgcccc tgagcaacca ggtaattttt cgctgtattt acagcaatcc gatacagcca
    18181 tgtataaaaa gcgctatctc cccggaacga atccagcgca cgataggctt taataaaagc
    18241 ttcttgtacc acatcgggaa catcacccga cggcacatag cgggaaacca gactcgccac
    18301 tttatgctga tagcgcacta ccagtaagtt aaaggctttc tgatctccct tctggacccg
    18361 ttcaaccagg acctggtccg ttaactgctc gctcatccga ggtaaagtct ccccaaacca
    18421 aatttccacg cgctatcgaa acgccactcc attagctgca atttgagcaa gcaaagggtt
    18481 agagtgtctc gtttttgtaa agttccgtaa cgcatctgtt tttgtttgtc atgctgtaga
    18541 cggatcatta tctatcatta taagtctaca gaatctgaac atcgcattat ctgtgtagaa
    18601 atgcccattt aactgcctga agagtaaccc aacggccttt ttatttcacc acctaatcct
    18661 ccaccagcca gtaacttctc tttttctcgc cgccctgcat cagcgtgttt agcaactgta
    18721 acaaatatta aaatagcagg tgtttattcg cacaacatga tgctatgctg accaaaccgt
    18781 gtttagtaaa ttaaacaaag aaaatgaata ctctccctga acattcatgt gacgtgttga
    18841 ttattggtag cggcgcagcc ggactttcac tggcgctacg cctggctgac cagcatcagg
    18901 tcatcgttct aagtaaaggc ccggtaacgg aaggttcaac attttatgcc cagggcggta
    18961 ttgccgccgt gtttgatgaa actgacagca ttgactcgca tgtggaagac acattgattg
    19021 ccggggctgg tatttgcgat cgccatgcag ttgaatttgt cgccagcaat gcacgatcct
    19081 gtgtgcaatg gttaattgac cagggggttt tgtttgatac ccacgttcaa ccgaatggcg
    19141 aagaaagcta ccatctgacc cgtgaaggtg gacatagtca ccgtcgtatt cttcatgccg
    19201 ccgacgccac cggtagagaa gtacaatcca cgctggtgag caaggcgcag aaccatccga
    19261 atattcgtgt gctggagcgc agcaacgcgg ttgatctgat tgtctctgac aaaattggcc
    19321 tgccgggcac gcgacgggtt gttggcgcgt gggtatggaa ccgaaataaa gaaacggtgg
    19381 aaacctgcca cgcaaaagca gtggtactgg caaccggcgg tgcgtcgaag gtttatcagt
    19441 acaccaccaa tccggatatt tcttctggcg atggcattgc tatggcgtgg cgcgcgggct
    19501 gtcgggttgc caatctcgaa tttaatcagt tccaccctac cgcgctgtat cacccacagg
    19561 cacgcaattt cctgttaacg gaagcactgc gcggcgaagg cgcttatctc aagcgcccgg
    19621 atggcacgcg ttttatgccc gattttgatg agcgcggcga actggccccg cgcgatattg
    19681 tcgcccgcgc cattgaccat gaaatgaaac gcctcggcgc agattgtatg ttcctcgaca
    19741 tcagccataa gcccgccgat tttattcgcc agcatttccc gatgatttat gaaaaattgc
    19801 tcgggctggg gattgatctc acacaagaac cggtaccgat tgtgcctgct gcacattata
    19861 cctgcggtgg tgtaatggtt gatgatcatg ggcgtacgga cgtcgagggc ttgtatgcca
    19921 ttggcgaggt gagttatacc ggcttacacg gcgctaaccg catggcctcg aattcattgc
    19981 tggagtgtct ggtctatggc tggtcggcgg cagaagatat caccagacgt atgcctgatg
    20041 cccacggcgt cagtacgtta ccgccgtggg atgaaagccg cgttgagaac cctgacgaac
    20101 gggtagtaat tcagcataac tggcacgagc tacggctgtt tatgtgggat tacgttggca
    20161 ttgtgcgcac aacgaagcgc ctggaacgcg ccctgcggcg gataaccatg ctccaacaag
    20221 aaatagacga atattacgcc catttccgcg tctcaaataa tttgctggag ctgcgtaatc
    20281 tggtacaggt tgccgagttg attgttcgct gcgcgatgat gcgcaaagaa agccgggggc
    20341 tgcattttac gctggattac ccggaactgc tcacgcattc cggtccatcg atcctttccc
    20401 ccggcaatca ttacataaac agataaaaag cctgggtcag cgccgtatac gcttcggaat
    20461 agttctggtc tggcccacga atgactaagc gatcgctaaa gcattctccc gcctgcgggg
    20521 agaatgccag cagcacccga tgcggcagtc gcgcttcgtt ttccgccaca tccgtccgca
    20581 aacgtaaatg ccagcccatg cttaatgcca gttcagtaaa accattacca atctgctctg
    20641 gcagcaccac gcagaaaaat ccctcttcgg taatgcactc cgccgcacag gtcagcagcg
    20701 atgggtgatc aagcgtagtg gtatagcgag cctgttcccg ttgaggtgtc gcgcactcta
    20761 ctccctgctg atagtaaggt gggttactga tgattaaatc gaagcgtact gtctgctgtg
    20821 tgatccactg ctgaatatcc gccgtatgga cgttaatccg ctctgcccac ggggactggt
    20881 tgatattttc ctgcgcctgc gcggcagctt cactttccag ctcaacggca tcaattatca
    20941 cgctgtcact ggttcgctgc gccagcatca gtgccagcaa cccgctaccc gcgccgatat
    21001 caaggcaacg tttcacccca gccaccggtg cccatgcgcc cagcaaaatg ccatccgttc
    21061 ccactttcat cgcacagcga tcgtgagcaa caaaaaactg tttaaaagta aatccattac
    21121 gacgaagcac ggatgtagac tgtgacatga aaataaaacc ttgcaggaaa aacggcgata
    21181 gcaccgggtg agaacaatac ccgagaagcg atatccatac aaacagatga agatttcagc
    21241 cgtaacgtct ataatcagcg ccccacacag aggtagaaca tgactgtaac gactttttcc
    21301 gaacttgaac tcgacgaaag cctgctggaa gccctccagg ataaaggttt cactcgcccg
    21361 accgccattc aggctgccgc cattccgcct gcgctcgatg gccgtgatgt actcggttct
    21421 gcgccgacag gcaccggtaa aacggcggcg tatctgctgc cagcgttgca gcacctgctc
    21481 gatttcccgc gtaagaaatc cggtccgccg cgtattttga tcctcacccc aactcgcgag
    21541 ctggcgatgc aggtggccga tcatgcccgc gaactggcga aacatacgca tctggatatc
    21601 gccaccatca ccggcggcgt agcctatatg aaccacgcgg aagtgttcag cgaaaaccag
    21661 gacatcgtgg tcgccacgac cgggcgtctg ctgcaataca taaaagaaga gaacttcgat
    21721 tgccgcgcgg ttgaaacgct gatcctcgac gaagcagacc gtatgctgga tatgggcttc
    21781 gctcaggata tcgaacatat tgctggcgaa acgcgctggc gtaaacagac cctgctcttt
    21841 tcggcaacgc tggaaggcga tgcgattcag gactttgccg agcgtctgct ggaagacccg
    21901 gtggaggttt ctgccaatcc gtccacccgt gagcgcaaaa aaattcatca gtggtattac
    21961 cgcgccgatg atcttgagca taaaaccgcg ttgctggtgc atctgttaaa acagccggaa
    22021 gcgacccgct caattgtgtt tgtgcgtaag cgtgaacgtg tgcatgagct ggcaaactgg
    22081 ctgcgcgaag cgggcatcaa caactgctat ctcgaaggtg agatggtaca aggcaagcgt
    22141 aacgaagcga tcaagcgttt gaccgaaggt cgcgtaaacg tactggttgc aaccgatgtt
    22201 gccgcgcgcg gtatcgacat tcctgacgtc agccacgtct ttaacttcga tatgccgcgc
    22261 agtggtgata cttatttgca ccgtatcgga cgtaccgcgc gcgccggtcg taagggaaca
    22321 gcgatttcgc tggttgaagc tcatgaccat ctgctgctgg gtaaagtagg ccgctatatt
    22381 gaagagccaa ttaaagctcg cgttattgat gagttacgcc cgaaaacgcg tgcgccaagc
    22441 gaaaagcaga ccggcaagcc atcgaagaaa gtactggcta aacgtgccga gaagaaaaaa
    22501 gctaaagaga aagagaagcc gcgggtgaaa aaacgccatc gcgacaccaa aaatattggt
    22561 aagcgccgta aaccaagcgg aacgggcgtg ccaccgcaaa cgacagaaga gtaatctcaa
    22621 tgccgggttt aagcctggta ttaaaagtgc tggcaaacgc aaaa
//
LOCUS       DAZKIV010000070        22252 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZKIV010000070 DAZKIV010000000
VERSION     DAZKIV010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22252
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.070"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            298..981
                     /locus_tag="ACHOT6_002621"
     CDS             298..981
                     /locus_tag="ACHOT6_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9947814.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1025..5875
                     /gene="ehaG"
                     /locus_tag="ACHOT6_002622"
     CDS             1025..5875
                     /gene="ehaG"
                     /locus_tag="ACHOT6_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFP9947815.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNATDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVELDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6243..7898
                     /gene="lldP"
                     /locus_tag="ACHOT6_002623"
     CDS             6243..7898
                     /gene="lldP"
                     /locus_tag="ACHOT6_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFP9947816.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7898..8674
                     /gene="lldR"
                     /locus_tag="ACHOT6_002624"
     CDS             7898..8674
                     /gene="lldR"
                     /locus_tag="ACHOT6_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFP9947817.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            8671..9861
                     /gene="lldD"
                     /locus_tag="ACHOT6_002625"
     CDS             8671..9861
                     /gene="lldD"
                     /locus_tag="ACHOT6_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFP9947818.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10047..10520
                     /gene="trmL"
                     /locus_tag="ACHOT6_002626"
     CDS             10047..10520
                     /gene="trmL"
                     /locus_tag="ACHOT6_002626"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFP9947819.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGAVLRD"
     gene            complement(10582..11403)
                     /gene="cysE"
                     /locus_tag="ACHOT6_002627"
     CDS             complement(10582..11403)
                     /gene="cysE"
                     /locus_tag="ACHOT6_002627"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFP9947820.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11483..12502)
                     /gene="gpsA"
                     /locus_tag="ACHOT6_002628"
     CDS             complement(11483..12502)
                     /gene="gpsA"
                     /locus_tag="ACHOT6_002628"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFP9947821.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12502..12969)
                     /gene="secB"
                     /locus_tag="ACHOT6_002629"
     CDS             complement(12502..12969)
                     /gene="secB"
                     /locus_tag="ACHOT6_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFP9947822.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(13032..13283)
                     /gene="grxC"
                     /locus_tag="ACHOT6_002630"
     CDS             complement(13032..13283)
                     /gene="grxC"
                     /locus_tag="ACHOT6_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFP9947823.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13425..13856)
                     /locus_tag="ACHOT6_002631"
     CDS             complement(13425..13856)
                     /locus_tag="ACHOT6_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFP9947824.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14101..15645
                     /gene="gpmM"
                     /locus_tag="ACHOT6_002632"
                     /gene_synonym="pgmI"
     CDS             14101..15645
                     /gene="gpmM"
                     /locus_tag="ACHOT6_002632"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFP9947825.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15679..16938
                     /gene="envC"
                     /locus_tag="ACHOT6_002633"
     CDS             15679..16938
                     /gene="envC"
                     /locus_tag="ACHOT6_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFP9947826.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16942..17901
                     /locus_tag="ACHOT6_002634"
     CDS             16942..17901
                     /locus_tag="ACHOT6_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFP9947827.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17888..18922)
                     /gene="waaH"
                     /locus_tag="ACHOT6_002635"
     CDS             complement(17888..18922)
                     /gene="waaH"
                     /locus_tag="ACHOT6_002635"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFP9947828.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19161..20186)
                     /gene="tdh"
                     /locus_tag="ACHOT6_002636"
     CDS             complement(19161..20186)
                     /gene="tdh"
                     /locus_tag="ACHOT6_002636"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFP9947829.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFMIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20196..21392)
                     /gene="kbl"
                     /locus_tag="ACHOT6_002637"
     CDS             complement(20196..21392)
                     /gene="kbl"
                     /locus_tag="ACHOT6_002637"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFP9947830.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21667..>22252)
                     /gene="yibB"
                     /locus_tag="ACHOT6_002638"
     CDS             complement(21667..>22252)
                     /gene="yibB"
                     /locus_tag="ACHOT6_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFP9947831.1"
                     /translation="IQKDESFTNRLEPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMG
                     LVKTPLVAWIDFGYCRKPNVTRGLKIWDFPFDESKMYLFTIKKGLTVTSQQQVFDFMI
                     GNHVYIIGGAIVGSQHKWKEFYKLVLESQKITLNNNIVDDDQGIFVMCYYKRPDLFNL
                     NYLGRGKWFDLFRCFRSNTLGAKMQALRIFLSRK"
BASE COUNT         5685 a         5754 c         5608 g         5205 t
ORIGIN      
        1 gtgaaatttt aatcatcata ttcacaaaat caattatctg taaaattttt cccagacatg
       61 gtttctttca tatttgacat ttattaataa tcatcattag aatccacgaa aaataatata
      121 ttcatacaaa cacagacatc ttttgaaacg aatgatacat ttttatgatg aatgcttaaa
      181 acaatattta atcgacacac atcactgtca ttaatgtaaa gcaacatagc tcaccaataa
      241 tacaatggac aactctaccg gcattgatcg ctggtcggag gaaataagga tttttacatg
      301 aacttgaaga agattttttt cagtgctgtc actgtttcag ttctctgcgc attaacggga
      361 tgtgactaca ttgaagaagg caagccagaa agtagtttac tgaaacaaca agaagaacat
      421 aataataaaa ttgtgctact tgaaaaacaa caagcacagc taaagagtca actcgaaact
      481 attcaaaaac aacaaactgg cataataaac agtacaaaga ctttaactca cgtaattaaa
      541 tccgttaaag accaacaaaa tacttttatt tttaccgagt ttaacccagc aaaaacgaag
      601 tatttcatcc tgaacaacgg ttcggtggct ttagcgggtc gagtgttatc cattgacgcc
      661 acagagaatg gtagtgttat tcatatctca ctggtcaact tattaagtac acctatctca
      721 aacattggtt ttaatgcgac atggggtggt gaaaaacctg tcgatgccaa agagtttgcc
      781 cggtggcaac aattactttt caacacatca atgaaatcca cattgaaatt attaccaggt
      841 caatggcaag acattaattt gaccctgaag ggtgtatcgc ccaataacct gggatatctg
      901 aagttagcca tcaacatgga aaatattcag ttcgacaatc ttccctctgc tgaaaatcgg
      961 cagaaaagaa gcaaaaaata aatgcagtta gtacgcttat aacaaggaat agcgagcatc
     1021 attaatgaac aaaatattta aagttatctg gaaccctgcg acagggaatt atactgttac
     1081 cagcgaaacg gcaaaaagcc gtggcaagaa atctgggcgc agtaagctgt taatttctgc
     1141 gctggttgcg ggtggaatgt tgtcgtcgtt tggggcattg gcgaatgccg ggaatgacaa
     1201 cggtcagggt gttgattacg gtagtggatc agctggcgac ggctgggttg ctataggcaa
     1261 aggggcgaaa gcaaatactt ttatgaacac cagtggttcc agtactgctg tgggttatga
     1321 cgctatagct gaaggccaat atagctctgc catcgggtca aaaacccatg cgattggcgg
     1381 tgcatcaatg gcctttgggg ttagtgcaat atcagaaggc gatagaagta tagcactggg
     1441 tgcctcttcg tattcattgg gccaatactc aatggccctc ggccgttatt caaaagcatt
     1501 gggtaaattg tctattgcta tgggggactc ttccaaagcg gaaggagcaa acgccattgc
     1561 cctgggaaat gccactaaag ctactgagat tatgagtatt gctcttggcg acaccgccaa
     1621 tgcgtcaaaa gcgtattcaa tggcgctggg agcaagtagc gtcgcatctg aagaaaacgc
     1681 aattgccctg gggcgtagca gtgtagctag cggtactgac agcctcgcat ttggcagaca
     1741 atcacttgcc agcgcagcga acgctattgc gataggtgct gagaccgaag ccgctgaaaa
     1801 tgcaactgct attggcaata atgcgaaggc aaaagggact aatagcatgg caatggggtt
     1861 cggaagcctt gccgataaag tcaatactat cgcattagga aatggcagcc aggctctggc
     1921 agataatgca atcgccatag gccagggcaa caaagctgat ggcgtggatg ccatcgctct
     1981 gggtaatggt agccagtcga gaggcttaaa caccattgcc ttaggcacag ccagtaatgc
     2041 aactggtgat aagagtcttg cgcttggtag taatagcagt gccaacggta ttaactctgt
     2101 cgcgctgggc gcagattcca ttgcggattt agacaatacc gtctctgtcg gcaatagttc
     2161 attaaaacgc aagatcgtta atgtgaaaaa tggcgcgatc aagtctgaca gttacgatgc
     2221 cattaatggt tcacagcttt atgccattag cgactcggta gcaaaaaggc ttggaggagg
     2281 ggctgcagta gatgttgatg acggtactgt tacagcacca acctacaatt taaaaaatgg
     2341 tagcaaaaat aacgtagggg ctgcgctcgc tgtacttgat gaaaacaccc tgcaatggga
     2401 ccaaaccaaa ggcaaataca gcgctgctca tggtactagt agcccaactg ccagcgtaat
     2461 caccgatgtt gcggatggca cgatttcagc ctccagtaag gatgcggtta acggttccca
     2521 actgaaagct accaatgacg atgtcgaagc caacaccgcc aatatcgcta ctaataccag
     2581 caacattgcc acgaatacag caagtattgc caccaatacc accaatatca ccaacctgac
     2641 ggattccgtt ggtgaccttc aggctgatgc cctgctctgg aacgaaacta aaaaggcatt
     2701 cagtgcagct cacggccagg ataccaccag caaaatcacc aacgttaaag atgccgacct
     2761 gacggctgac agcactgatg ctgttaacgg ctctcagctg aaaaccacca acgatgctgt
     2821 ggcgacgaat accaccaata tcgccaataa cacttccaat attgccacta acaccaccaa
     2881 catctctaat ctgactgaga cggtgactaa tcttggtgag gatgcgctga aatgggataa
     2941 ggacaatggt gtattcacgg cagctcatgg caccgagacc accagcaaaa tcaccaacgt
     3001 taaagatggc gacctgacga ctggcagcac cgatgccgtt aacggctctc agctgaaaac
     3061 caccaacgat gccgtggcga cgaataccac caatatcgcc actaacacca ccaacatctc
     3121 taatctgact gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa
     3181 tggtgtcttc actgcagctc atggcaacaa taccgccagc aaaatcacca atatcctgga
     3241 cggcacagtc actgcaacca gttccgatgc cattaacggt agccagcttt atgacttaag
     3301 cagcaatatc gccacctact tcggcggcaa tgcttctgtg aatactgacg gtgtgtttac
     3361 cggtccaacc tacaaaatcg gtgaaacaaa ttattataac gtcggcgatg cactggctgc
     3421 gattaactcc tcatttagca cgtctctcgg cgatgctctg ctttgggatg ccaccgcagg
     3481 taaattcagt gccaaacacg gtactaatgg tgacgcaagc gtgatcactg atgtcgcaga
     3541 tggtgaaatt tcagactcca gttctgacgc agtaaacggc tcacaactcc acggcgtgag
     3601 cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc aatgcagacg gcaccatcac
     3661 tgcgccgacg tacaccattg ctaatgctga ttacgataat gtcggtgatg ccctgaatgc
     3721 tatcgatacc actcttgacg acgctctgct ctgggatgcg gacgccggtg aaaatggtgc
     3781 atttagcgcc gctcacggaa aagataaaac tgccagtgta atcactaacg tcgctaacgg
     3841 tgcaatctct gctgccagca gcgacgcgat taacggctca caactctata ccaccaataa
     3901 gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac gctgacggca ccatcaccgc
     3961 accgacttac accattgcga acgccgagta caacaacgtc ggtgacgccc tggatgcgct
     4021 tgatgataac gccctgctgt gggatgagac tgccaatggc ggtgctggag cctacaatgc
     4081 cagccatgac ggtaaagcca gcatcatcac taatgtcgct aatggcagta ttagtgagga
     4141 cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg aatatgatga ttgagcagaa
     4201 cacccaaatt atcaatcagc tcgctggtaa caccgacgca acctatatcc aagaaaacgg
     4261 tgcgggtatt aactatgtgc gtactaacga cgacggctta gcgttcaacg acgccagcgc
     4321 acagggtgtt ggcgctacag ctataggtta taactctgtc gccaaaggcg atagcagcgt
     4381 agctattggt cagggcagct acagcgacgt tgatacgggt atcgccctgg gtagcagctc
     4441 tgtttccagc cgagtgattg ccaaaggctc ccgtgacacc agcataacgg aaaatggcgt
     4501 tgttattggt tacgacacca cggatggcga actgctcggt gcattgtcta tcggtgatga
     4561 cggtaaatat cgtcaaatca tcaacgtagc cgatggttcc gaagcccatg acgccgttac
     4621 ggttcgtcaa ttgcagaatg cgattggtgc ggtcgcaacc acgccgacta aatacttcca
     4681 cgctaattca acggaagaag attcactggc agtgggaact gactcgctgg caatgggtgc
     4741 gaaaaccatc gtgaatggcg ataaaggtat tggtatcggt tatggtgcct acgtggacgc
     4801 gaatgcactt aacggcattg ccattggtag caatgcgcaa gtcattcatg tcaacagtat
     4861 tgcgataggt aatggttcta cgaccactcg tggcgctcaa accaattata ccgcctacaa
     4921 catggacgca ccgcagaact ctgtcggtga attctcagtc ggtagtgcgg atggtcaacg
     4981 tcagatcact aacgtcgcag caggttcggc tgataccgat gcggtcaacg tgggtcagtt
     5041 gaaagtaacg gatgcgcagg tttcccagaa tacccagagc attactaacc tggataatcg
     5101 ggtaacgaat cttgattcac gcgtcaccaa tatcgaaaac ggtattggcg atatcgtcac
     5161 caccggtagc accaagtact tcaagaccaa taccgatggt gtagatgcca gcgcgcaggg
     5221 taaagatagc gtcgcgattg gttccggctc cattgctgcc gctgacaaca gcgtcgctct
     5281 gggtacaggg tctgtggcaa ccgaagaaaa tacgatctct gtaggttcct ctactaatca
     5341 acgtcgtatc accaacgtag ctgcaggtaa aaatgctacc gatgctgtta acgtggcaca
     5401 gttgaagtct tccgaagctg gcggtgtacg ttacgacacc aaagctgatg gttctatcga
     5461 ctatagcaat atcaccctcg gtggcggcaa cggcggtacg actcgtatca gcaacgtctc
     5521 cgctggcgtc aacaacaacg acgcggtgaa ttacgcgcag ttgaagcaaa gcgtgcagga
     5581 aacgaagcaa tacaccgatc agcgaatggt tgagttggat aacaaactgt ctaaaactga
     5641 aagcaagttg agcggtggta tcgcttctgc aatggcaatg accggtctgc cgcaggctta
     5701 cactccaggt gccagcatgg cctctattgg tggcggtact tacaacggtg aatcggcagt
     5761 tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg gtctacaaat tacaaggtag
     5821 taccaatagc cagggtgaat actccgccgc actcggtgcc ggtattcagt ggtaatgatc
     5881 cattaagtta gtgtgactaa gggcaaacgc caaattgcct gatgcgttac gcgcatcagg
     5941 caagataagg cgctccgggt caacaatcta agggcaattc tctgatgagg attgcccttt
     6001 tctttaccag acatctcccc ccacaagaat tggccctacc aattcttcgc ttatctgacc
     6061 tctggttcac aattccccaa ttaaaactca catcaatatt gccaatacat aacatttagt
     6121 taaccattca ttgtcattat ccctacacaa cacaattggc agtgccactt ttacacaacg
     6181 tgtgacaagg agatgagcaa cagactcatt acacgatgtg cgtggactcc aggagacctg
     6241 caatgaatct ctggcaacaa aactacgatc ccgccgggaa tatctggctt tccagtctga
     6301 tagcatcgct tcccatcctg tttttcttct ttgcgctgat taagctcaaa ctgaaaggat
     6361 acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt ggctttgctg ttctataaaa
     6421 tgccggtcgc taacgcgctg gcctcggtgg tttatggctt cttctatggt ttgtggccca
     6481 tcgcgtggat cattattgca gcagtgttcg tctataagat ctcggtgaaa accgggcagt
     6541 ttgacatcat tcgctcgtct attctttcga taacccctga ccagcgtctg caaatgctga
     6601 tcgtcggttt ctgtttcggc gctttccttg aaggagccgc aggctttggc gcaccggtag
     6661 caattaccgc cgcattgctg gtcggcctgg gttttaaacc gctgtacgcc gccggactgt
     6721 gcctgattgt taacaccgcg ccagtggcat ttggcgcgat gggcattcca atcctggttg
     6781 ccggacaagt aacaggcatc gacagttttg agattggcca gatggtgggg cgtcagctac
     6841 cgtttatgac cattatcgtg ctgttctgga tcatggcgat tatggacggc tggcgcggta
     6901 tcaaagagac gtggcctgcg gtcgtggttg cgggcggctc gtttgccatc gctcagtacc
     6961 ttagctccaa cttcattggg ccggagctgc cggacattat ctcttcgctg gtatcactgc
     7021 tctgcctgac gctgttcctc aaacgctggc aaccagttcg cgtattccgc ttcggtgact
     7081 tgggggcgtc acaggttgat atgacgctgg cccacaccgg ttacaccgcg ggtcaggtgt
     7141 tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt aacactgtgg agcatcccgc
     7201 cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga gtgggtgatc aacattccgg
     7261 tgccgtacct cgataaactg gttgcccgta tgccaccagt ggtcagcgag gctacagcct
     7321 atgccgccgt gtttaagttt gactggttct ctgccaccgg caccgccatt ctgtttgctg
     7381 cactgctctc gattgtctgg ctgaagatga aaccgtctga cgctatcagc accttcggca
     7441 gcacgctgaa agaactggct ctacccatct actccatcgg catggtgctg gcgttcgcct
     7501 ttatctcgaa ctattccgga ctgtcatcaa cactggcact ggcactggcg cacaccggtc
     7561 atgcattcac cttcttctcg ccgttcctcg gctggctggg ggtattcctg accgggtcgg
     7621 atacctcatc taacgccctg ttcgccgcgc tgcaagccac cgcagcacaa caaattggcg
     7681 tctctgatct gttgctggtt gccgccaata ccaccggtgg cgtcaccggt aagatgatct
     7741 ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct ggtgggcaaa gagtctgatt
     7801 tgttccgctt tactgtcaaa cacagcctga tcttcacctg tatggtgggc gtgatcacca
     7861 cgcttcaggc ttatgtctta acgtggatga ttccttaatg attgttttac ccagacgcct
     7921 gtcagacgag gttgccgatc gtgtgcgggc gctgattgat gaaaaaaacc tggaagcggg
     7981 catgaagttg cccgctgagc gccaactggc gatgcaactc ggcgtatcac gtaattcact
     8041 acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg ctcagtcgac gcggcggcgg
     8101 gacgtttatt cgctggcgtc atgacacatg gtcggagcaa aacatcgtcc agccgctaaa
     8161 aacgctgatg gccgatgatc cggattacag tttcgatatt ctggaagccc gctacgccat
     8221 tgaagccagc accgcatggc atgcggcaat gcgcgccaca cctggcgaca aagaaaagat
     8281 tcagctttgc tttgaagcaa cgctaagtga agacccggat atcgcctcac aagcggacgt
     8341 tcgttttcat ctggcgattg ccgaagcctc acataacatc gtgctgctgc aaaccatgcg
     8401 cggtttcttc gatgtcctgc aatcctcagt gaagcatagc cgtcagcgga tgtatctggt
     8461 gccaccggtt ttttcacaac tgaccgaaca acatcaggct gtcattgacg ccatttttgc
     8521 tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac cttagttttg ttcacaccac
     8581 catgaaacga ttcgatgaag atcaggctcg ccacgcacgg attacccgcc tgcccggtga
     8641 gcattatgag cattcgaggg agaaaaacgc atgattattt ccgcagccag cgattatcgc
     8701 gccgcagcgc aacgcattct gccgccgttc ctgttccact atatggatgg tggtgcatat
     8761 tctgaataca cgctgcgccg caacgtggaa gatttgtcag aagtggcact gcgccagcgt
     8821 attctgaaaa acatgtctga cttaagcctg gaaacgacgc tgtttaatga gaaattgtcg
     8881 atgccggtag cactggcacc ggtgggtttg tgtggcatgt atgcgcgtcg tggcgaagtt
     8941 caggcagcca aagcggcgga cgcgcatggc attccgttta ctctctcgac ggtttccgtt
     9001 tgcccgattg aagaagtcgc gccagccatc aagcgcccaa tgtggttcca gctttatgta
     9061 ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag caaaagcagc gggttgttcg
     9121 acgctggttt tcaccgtgga tatgccgaca ccgggcgcac gctaccgtga tgcgcattca
     9181 ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc aggcggtgac gcatccgcaa
     9241 tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt taggtaatat ctcagcttat
     9301 ctcggcaaac cgaccggact ggaagattac atcggctggc tggggaataa cttcgatccg
     9361 tccatctcat ggaaagacct tgagtggatc cgcgatttct gggatggccc gatggtgatc
     9421 aaagggatcc tcgatccgga agatgcgcgc gatgcagtac gttttggtgc tgatggaatt
     9481 gtggtttcta accacggtgg ccgccagctg gacggtgtac tctcttccgc tcgtgcgttg
     9541 cccgctattg cagatgcggt gaaaggtgat atcgccattc tggcggatag cggaattcgt
     9601 aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg acaccgtact tctgggtcgt
     9661 gctttcctgt atgcgctggc aacagcgggc caggcgggtg tagctaacct gctaaatctg
     9721 atcgagaaag agatgaaagt ggcaatgacg ctgactggcg cgaaatcgat cagtgaaatt
     9781 acgcaagatt cgctggtgca ggggctgggt aaagagttgc ctacggcact ggctccgatg
     9841 gcgaaaggga atgcggcata gtcgtttgag ccccctcacc ctaaccctct ccctcaggga
     9901 gtggggaccg ttcggcgctg tatgtactcc ctcactctga aacgacaccg cactcttttt
     9961 ttctccctcg cccctccggg gtgaggggaa aaggccgcac tgctcaccaa ttttctgcta
    10021 tcctgccccc gcactaaggg ggcagtatgc taaacatcgt actttacgaa ccagaaattc
    10081 cgccaaatac tggcaatatc atccgtcttt gcgctaatac cggctttcgt ctgcatatca
    10141 tcgaaccgat gggatttgcc tgggacgata agcgcctgcg ccgcgcgggg ctggactatc
    10201 acgagtttac cgccgttacg cgtcatcatg actttcgcgc gttcctcgaa gcagaaaatc
    10261 cccagcgcct gtttgccctc accacgaaag gtacgcctgc tcacagcgcc gtaaactatc
    10321 aggatggcga ttatctgatg ttcggcccgg aaacacgcgg cctgccagcg agcattcttg
    10381 atgccctgcc cgctgaacaa aaaattcgca ttccgatggt gccggacagc cgcagcatga
    10441 acctgtccaa tgcggtgtca gttgtggtgt atgaagcctg gcggcagttg gggtatccgg
    10501 gagcggtgtt gagagattag ttacgtcatg ccggataaga tgcgttatga accgcatcac
    10561 atccggcacg accacaggac atcagatccc atccccatac tcaaatgtat ggttaatacc
    10621 gttgaaatgc tggtccatat ccattgatgg cttatcgctg tctggtttac cgacaatacg
    10681 agccggaacg ccagcggcgg ttgtatgcgg cggcaccggt tgcagcacca cggaacctgc
    10741 gccaatcttc gcgccgcgac caacttcaat attgccgagg attttcgcgc ccgcgccaat
    10801 catcacacct tcacgaattt tcgggtgacg gtcaccacca gatttacccg taccgccaag
    10861 cgtcacagat tgcagaatcg atacgtcgtt ttcaattacc gccgtttcac ccacaacgat
    10921 gcccgtggcg tggtcgagca taatgccacg gccaattttt gctgccgggt gaatatcgac
    10981 ctggaacgtc acagaaacct ggttttgcag aaagattgcc aatgcgcgac gcccctgatt
    11041 ccacaaccag tgaccgatgc gataggcctg caaggcatga aaacccttca ggtataacaa
    11101 cggggttgag tatttatcga ctgccgggtc gcgggtacgc accgcctgaa tatcacaggc
    11161 cgcagaggcg atcatttccg ggtcagcggc gtaggcttct tccaccactt cacggatagc
    11221 aatagcaggc ataattggcg atgacagctt gttcgccagc atgtagctca gtgcactacc
    11281 aaggttttcg tgcttgagta gcgtcgcgtg gtaaaaactg gccagcattg gctcacagtc
    11341 cgccagcgtt ctggcttcgg ctttaatatt gttccagaca atttccagtt cttcacacga
    11401 cattgcttac tccacacgat gagataatga ccggcccgtt ctgcgcgggc cgggtcatag
    11461 cggtaacaaa ggttccctgg gtttagtggc tgctgcgctc gtccttgcgt gcacgaccta
    11521 ataaagtcaa tgctgcctcg cgcgcgtttt ttccgcaata taatacttga taaatttcct
    11581 cggttattgg catttcaacg ccgaagcgat gcgccagttc gcggacttct ttcgtattgc
    11641 ggtagccttc caccacctga ccaatcttct cctgcgcgct ttgtacatcc atgccctgac
    11701 cgagcatcat gccaaaacgg cggttacgcg actggttgtc ggtacaggta agcaccagat
    11761 cgccaagccc cgccatgccc ataaaggtgg caggatcggc acccagcgcc gcaccaagac
    11821 gcgacatttc agccagccca cgggtgatca gcgccgtacg cgcattcgca ccaaaaccga
    11881 taccgtcaga catccccgcg ccaatggcaa tgacgttttt caccgcaccg ccaagctgca
    11941 caccgatgaa atccgggttg ctgtaaacgc ggaaactttt gccacagtgc agcaattgtt
    12001 ggagatcatc ggcaaaggtc tggtcggtag aggccaacga aatagccgtc ggtaaacctg
    12061 ccgccagttc tttcgcaaac gttgggccag agataaccgc cagcggaatt tgatcgccta
    12121 aggcctcacg cgccacgtcc tgtaacagac gtccggtttc cgcttccagc cctttggtcg
    12181 cccacaccag acgcgcatca ggacgcatca gcggtttaat ctggcgcagc acttcaccaa
    12241 agacatggct gggtacgacg acgagaatat tacggctggc tgccagcgca gtggcgagat
    12301 cgctttcaag atggagcgta tcgggaaaag gcacatcggg gagaaacgcg gcgttacagc
    12361 ggtcgcgttc aagcgttgcg atatgttcag ggtcatggcc ccagaggaca acctcgtggc
    12421 catttcttgc cagggtgatg gcaagagcgg tgccgtacga gccggcaccg atcacagtca
    12481 ttgaagcatt acgttggttc atcaggcatc ctgatgttct tcagtacctt cgccagcctg
    12541 ctgctgcaaa tagttcatga acagcgcatc gaagttaacc ggcgcaaggt tcagttgcgg
    12601 gaatgtaccg cgggatacca tgctggtgat gcactcacga gcatacggga acagaatgtt
    12661 cgggcagtat gcgcccaggc aatgtgccat ctgggtgcct tcgatacccg cgatggagaa
    12721 aataccgccc tgctgaactt cacacaggaa cgcggtttct tcgcccaggg aggccgttac
    12781 ggtaacacgc agtaccactt cgtaaacgtc atctgccagt tgggaagatg ccgtatccag
    12841 atcaagttta acttctggct gccaatcttt ctggaaaacg tgcggcgcgt tcggcgcttc
    12901 gaaagaaata tccttggtat aaatacgttg gatctggaaa gtcatttcag tgttgttttg
    12961 ttctgacatg tgtagaaaac ccttaagtgt tgtccttaaa tactgcgcaa tgtaatgcca
    13021 acgttcacac gttatttcag caggggatcc agtccaccac gtgcatccaa tgcatacaag
    13081 tcatcacagc cgccaatgtg ctgtgcgtca ataaaaatct ggggaaccgt ggtgcgaccg
    13141 ctgcgtttga tcatctcttc acgcttggcg gcattgccat cgatcggcaa ctcctgaaaa
    13201 ctcacgccct tgctgctcag cagtgctttt gcacgatggc aatacgggca ggtttctttg
    13261 gtatagattt caacattggc cataatttag ctcctgtttt tttaccctga aatttcatgt
    13321 tgcagggcgg ctgcaaaatg gagtgtcccc agaagcttat aacaataagt cgctgggggc
    13381 ctaattgcag ctaacggcct gcatcatgaa agacgacagg taaattattt gccgcgcacc
    13441 aaaggcaagt tttcgccagc ccagccagcg acgccttctt tcagcacgaa tacttgcgca
    13501 aaaccagctt tcgtcagtgc gtttgcaggc tcctggcact gcatgccaga accgtctacc
    13561 acgataaccg gtttgtcttt gtgcttctca agctcaccaa cattgttggc tttgatttcg
    13621 ctcggcaaca gattgataga acctgcgata tggcctttac ggaagtcatc acgctgacgt
    13681 aaatccacaa ccacagcgtc ttctttgttg atcagacgcg tagcttcacc acgagtaatc
    13741 accttcactt tcgaggtcag gctcttaaac gtagtcacaa gaaccgccac cagtaacgcg
    13801 atccaggcga tactcagtat gggatgacgg ccaacaaatt gcataatttc ttgcatgggg
    13861 ggtaacaact cccgacgtag tgattaaaaa aaccaggaaa ggagtatacc tgcgcggtgc
    13921 ggcaaataca gccagcgcgt taactggaat gcaattttgc ggggcacgac gaaaaaaaag
    13981 cgcaaatctg gcatcggctt gccgcgagcg gtcgtatatt ttgatcttca gaggctattt
    14041 tatcgattca gctgtagtaa aattacgcaa attttgactc ttgagtatga ggttgtcgca
    14101 atgtcggttt ctaaaaaacc tatggtactg gtgattctgg atggctatgg ctatcgtgaa
    14161 gaacagcagg ataacgccat ttttagtgct aaaaccccgg taatggatgc actgtgggcc
    14221 aatcgtccgc ataccctgat cgacgcttcc ggtctggaag tcggtctgcc tgaccgtcag
    14281 atgggtaact ccgaagtagg ccacgttaac ctgggtgccg gccgcatcgt gtatcaggac
    14341 ctgactcgtc tggacgttga aatcaaagat cgcgctttct ttgctaatcc agtgctgact
    14401 ggcgcggtag ataaagcgaa aaacgcaggt aaagcagtac acattatggg tctgctctcc
    14461 gcaggcggcg tacacagcca cgaagatcac atcatggcga tggtagaact ggcagctgaa
    14521 cgcggcgcag aaaaaatcta cctgcacgca ttccttgacg gtcgcgacac cccgccgcgc
    14581 agtgctgaat cctcgctgaa aaaatttgaa gagaaatttg ctgcgctggg taaaggccgc
    14641 gtagcgtcca tcattggtcg ttactacgcg atggaccgcg ataaccgttg ggatcgcgtt
    14701 gaaaaagcct atgacctgct gactctggcg cagggtgagt tccaggccga tactgccgtt
    14761 gctggtttgc aggcagctta tgctcgcgac gaaaacgacg agttcgtgaa agcgaccgtt
    14821 attcgtgcag aaggtcagcc agatgcggca atggaagacg gcgatgcgct gattttcatg
    14881 aacttccgtg ctgaccgcgc gcgcgaaatc actcgtgcat tcgtaaacgc agatttcgac
    14941 ggcttcgcgc gtaagaaagt ggttaacgtc gatttcgtga tgctgaccga atacgccgct
    15001 gacatcaaaa ctgcggttgc ttacccaccc gcttccctgg ttaacacctt cggcgagtgg
    15061 atggcgaaaa acgacaaaac tcagttgcgt atttccgaaa ccgaaaaata tgcccacgtt
    15121 actttcttct tcaacggtgg cgtagaagag tcgttcaaag gcgaagatcg cattctgatc
    15181 aactcaccga aagtggctac ctacgatctg caaccggaaa tgagctccgc agagctgacc
    15241 gaaaaactgg ttgcggccat caagagcggc aaatacgaca ccattatctg taactatccg
    15301 aacggcgaca tggtaggtca caccggggtg atggaagcgg cggttaaagc ggttgaagca
    15361 ctggatcact gcgtggaaga agtcgcgaaa gcggttgagt ccgtgggtgg acaactgctg
    15421 atcaccgctg accacggtaa cgctgaacag atgcgcgatc cggcaacggg tcaggcacac
    15481 acggcacaca ccaacctgcc agttccgctg atttatgttg gtgataagaa cgtgaaagcg
    15541 gttgaaggcg gcaaactttc tgacatcgcg ccgaccatgt tgtcgctgat gggtatggaa
    15601 atcccgcaag agatgactgg taagccgctg ttcatcgtgg aataatccct ccccatgagg
    15661 ggaaaggcga ttaataccat gacacgggcc gtgaaaccgc gcaggtttgc aatcaggccc
    15721 atcatctacg ccagcgttct tagcgctggc gtattgttgt gcgccttttc cgcccacgcg
    15781 gatgagcgtg accaactcaa atctattcag gccgatatcg ccgcaaaaga gcgcgcggta
    15841 cgccaaaagc aacaacaacg cgcaagcctg ctcgcacaat tgaaaaagca ggaagaagcg
    15901 atctctgaag ccacccgtaa gctgcgcgaa acgcaaaaca cgctcaatca actcaataaa
    15961 cagattgatg agatgaacgc gtcgattgcc aaactggagc agcaaaaagc cgcccaggag
    16021 cgcagcctcg ccgcgcaact ggatgccgca ttccgtcagg gcgagcatac cggtattcag
    16081 ctaattctca gcggtgaaga aagccagcgt ggacagcgtt tacaggctta tttcggctat
    16141 ctcaaccagg cgcgacaaga aaccattgcc cagttgaagc aaacgcgtga agaagtcgcc
    16201 atgcagcgtg ctgaactgga agagaaacaa agcgagcaac aaacgctgtt atatgaacag
    16261 cgcgcccaac aggcgaaact gactcaggct ctgaacgagc gtaaaaagac gctggcgggg
    16321 ctggaatctt ccatccagca aggtcagcag cagctaagtg aactgcgcgc caacgaatcc
    16381 cgtctgcgta acagcattgc ccgtgcggaa gccgcggcga aagcgcgtgc tgaacgagaa
    16441 gcgcgtgagg cccaggcggt tcgcgaccgc cagaaagaag cgacgcgcaa aggcaccacc
    16501 tataaaccga ccgaaagcga aaaatcgctg atgtcccgaa ctggtggcct gggtgcgccg
    16561 cgtggtcagg cattctggcc ggttcgcggg ccgacgctgc atcgctatgg tgaacagcta
    16621 cagggcgaac tacgctggaa aggaatggtt atcggtgcct ctgaaggtac tgaagttaaa
    16681 gcgattgccg acggtcgggt gattctggct gactggctgc aaggttacgg tctggtggtg
    16741 gtggttgagc acggtaaagg cgacatgagt ctttacggct ataatcagag cgcactggtg
    16801 agcgttggtt cgcaggttcg cgcgggccag ccaattgcac tggtgggcag cagtggcggt
    16861 cagggtcggc cttcactcta tttcgaaatt cgccgccagg gtcaggcggt caatccacag
    16921 ccgtggttgg gaagataagt tttgtttcca tttcgtcgta acgttcttgc atttgccgct
    16981 ctgttggcgc tctcctcccc cgtacttgct ggcaaacttg ccatcgtcat tgatgatttt
    17041 gggtatcgcc cgcacaacga aaaccaggtg ctggcgatgc cctccgctat ctccgtcgct
    17101 gtattacccg attcaccgca cgccagagag atggcgacca aagcgcataa cagcgggcac
    17161 gaagtgttga ttcatctccc gatggcacca ctgagtaaac agccgctgga gaaaaatacg
    17221 ctgcgcccgg agatgagcag cgacgaaatt gagcgcatta ttcgtagtgc ggtcaataac
    17281 gtgccctatg ccgtggggat caacaaccac atgggtagca agatgacctc taacctgttt
    17341 ggtatgcaga aagtgatgca ggcactggag cgttacaatc tttacttcct cgacagcgta
    17401 accatcggta atacccaggc gatgcgcgcc gcgcaaggta ctggcgtgaa ggtgatcaaa
    17461 cggaaggtgt tcctcgacga ttcgcagaac gaagcggaca tccgtgtgca atttaatcgc
    17521 gcaattgatc tggcgcgtcg caacggttca accattgcca ttgggcatcc tcacccttca
    17581 acggtacgcg tgctacaaca gatggtttat aacctgccgc cagacattac gctggtaaaa
    17641 gccagcagct tgctgaatga accgcaggtt gatacttcta caccgccgaa aaacgctgtg
    17701 cctgatgcac cgcgtaatcc gttccgtggc gtgaagctgt gcaaaccgaa gaaaccgata
    17761 gaacctgttt atgctaatcg cttctttgaa gtattaagcg aaagcatcag ccagagcacg
    17821 ctgatcgttt acttccagca tcagtggcaa ggctggggca aacagcccga agcggcgaag
    17881 tttaacgcta gcgcaaatta aaggcgctac gcgtaatgcg atggtgcgac cgcgttttgt
    17941 cgcgccattg ccataagcgg aaagaccaca gtaacgcctg gtaaccgacc ttcacgctgc
    18001 gcacattggt aatcaggcgc ttatacatac cggaagtaaa tatctcggca atcatccgtt
    18061 ggcgggtaag aatatccggc tctttgcgca ccgcatggca aacacgcaat gcttcgtaag
    18121 ttatttgctg atgaaattcc ggataaatcg taattttgtc ggcataattt cgatttaatt
    18181 tctccagcag gcgggtaatc ttaatatagt gacgttgata attaaggttt ttattccctt
    18241 gtctatgcaa ccgactcact gacgtattat gcagataata tttatataat gattgctcgg
    18301 tatatcgcgc acgcagcgcg ttaaacatga attctgttgt ccagacaata tcctgatgat
    18361 gtaatccggc aataaattta atgttatttt taacaataac atcacggcga taaaccccca
    18421 tccagacaac gtgagtccaa cggcgcgaag aaagccccat ccgcagccag tccgggccgg
    18481 ttaatacgcc ggttgagcga aggcgatcgc tggggatgga ttgccaggtt tctcccgttt
    18541 cacgaaaaca ccagtcagcg ttgcactgcg ccacgtcgag gtcatcctct aacgccatgg
    18601 tcatcagcgt ttcgtacatg gtgggataga cctcatcgtc agcatcgaca aaagcgacat
    18661 atttgcccgt cgccacctcg atcccacgat tacgcgctac cgatgcgcca gcattcgcct
    18721 gatgcaacaa acgaacgtgc ggatagtttt ctgcgtaatg ctttgctatt tcaacagaat
    18781 tatccgttga accatcgtta ataataatga tttccagagc agtccaggtt tgcgtaatta
    18841 aagattccat acaagtgcgg aaatcatcgc ccgcattata taacggaata ataacactaa
    18901 gtttattggt gctgttcatc ataaattacc gacgacctct gaagttatcc tgacgtttta
    18961 caggctttgt attaagaaac tattaagcct gtgagatatt atttttacag gtgagagagt
    19021 cacttcaggg tagagagata atgagagcag ctatataaga gatcagcgca attacgatat
    19081 ctttattaca ctgacaggat tttacatatt taacggatta tcctcaggca aaagggctgg
    19141 aataccagcc cttgttcgtg ttaatcccaa ctcagaatta ctttcccgga ctggcccgaa
    19201 cgcatagcgt caaagccctt ctggaaatca tcgatagaga aacgatgggt aatgatcggc
    19261 gagagatcca gacctgactg aatcagcgcc gccatcttgt accaggtttc aaacatctca
    19321 cgaccgtaaa tacctttaat gaacaagcct ttaaagatta ctttggtcca gtcgatagac
    19381 atatcggacg gtggaatacc cagcatcgca atgcgaccgc cgtggttcat ggtgtcgagc
    19441 atggtacgaa acgctggcgg cgcaccggac atttccagac cgacatcaaa gccttcggtc
    19501 atgcccagtt ctgccatcac atcattaagg ttttctttag cgacgttgac cgcacgggtg
    19561 atacccattt tgcgcgccag ttcgagacga tattcgttaa catcagtgat caccacattg
    19621 cgtgcaccaa cgtgtttcgc caccgccgct gccataatac caatcgggcc tgcaccagag
    19681 accagcacat cctcgccaac cagatcgaac gacagcgcgg tatgcacggc gttaccgaag
    19741 ggatcaaaaa tggaagccag gtcgtcggag atattgtcgg gaattttgaa ggcgttgaac
    19801 gccggaatca ccagatattc ggcgaagcag cccgggcggt taacgccgac gccgatcgtg
    19861 ttgcggcaca gatgggtacg accgccacga cagttgcgac agtgaccgca ggtgatatgg
    19921 ccttcgccag aaacgcgatc gccaatcatg aagcccttca cttcctgacc aatacctacc
    19981 acttcaccga catattcatg gcccacgacc atcggcaccg ggatggtttt ttgcgaccac
    20041 tcatcccagt tatagatgtg aacgtcagtc ccgcagatgg ctgttttacg gattttaatc
    20101 agcagatcgt tatgcccgag ttccggtaca ggaacgtcgg tcatccagat gccctcttcc
    20161 gctttcagtt tggataacgc tttcatctca catcctcagg cgataacgcc cagttgttta
    20221 ccaatacgcg tgaatgcttc tactgcacgc gtaatttgct caggggtatg cgccgcagac
    20281 atctgggtac gaatacgcgc ctgacctttc ggaacgaccg gatagaagaa accggtgacg
    20341 taaatgccct ctttttgcag ctctcgggca aatttctgcg ccactaccgc atcaccaagc
    20401 atgaccggaa taatggcgtg atcggctccc gccagggtaa agcccgccgc cgacatttgc
    20461 tcacggaact gacgcgcgtt cgcccacaga cggtcacgca gttcgctgcc cgcttcgacc
    20521 atctccagta ctttgatgga cgcggcaaca atggccggtg ccagcgagtt ggagaacagg
    20581 tacggacgag aacgctggcg cagccactca accacctctt tgcgcgccgc ggtataacca
    20641 ccagaagccc cgcccagcgc tttaccaagt gtgccggtga taatatcgac ccggcccatc
    20701 acatcgcagt attcatggga accacgaccg ttttcaccga caaaaccgac cgcgtgggag
    20761 tcgtctacca tcaccagggc atcgtattta tctgccagat cgcaaacgcc cttcagattg
    20821 gcaatcacgc cgtccatcga gaacacacca tcggtggcaa tcagcacatg acgcgcaccg
    20881 gcttcacgcg cttctttcag acgagcttca agctcctgca tatcgttgtt ggcatagcga
    20941 tagcgtttag ctttacacag acgcacacca tcaataatag acgcgtggtt cagtgcgtcg
    21001 gagataatgg cgtcttccgc gcccagcagc gtttcaaaca ggccaccgtt agcatcaaag
    21061 caggaagagt agagaatcgc atcttccatc cccaggaatg ccgccagttt ttgttcaagc
    21121 tctttatggc tgtcctgagt gccgcaaata aaacgcaccg aagccatgcc gaaaccgtga
    21181 gaatccattc ccgcctttgc tgccgcaatc agatccggat gattcgccag cccgagatag
    21241 ttgttggcac aaaagttaat gacgtggctt ccatcagcca cagtgatatc tgcttgctgc
    21301 gcagacgtaa taatgcgctc ttctttaaac aacccttccg cccgtgcggt ttccagatcg
    21361 ttggttaact gctgataaaa ttctccacgc attgcgattc tccagacagg gcaaattcca
    21421 gcacatatta cccaaactta tacgtcgcga cgagatgacg cgttaacact tctgcaaaat
    21481 tcaggataaa taacgtgtaa cttgatgtct ctcaacacct acagatagta gaaaaactgt
    21541 acagttttgg ctgggcataa tgatgattga ttttactgca ttgatataaa aagaaaaaat
    21601 gatgccaaag catcattttt cctatctcag ttggtatcaa aacgctaccc actaatggag
    21661 ggaaacctat tttcttgaaa ggaaaattct cagcgcctgc atttttgccc ctaaagtgtt
    21721 actcctgaag cagcgaaaca aatcgaacca ttttcctctc cccagatagt ttaggttgaa
    21781 gagatcgggg cgtttgtaat agcacatgac aaaaatgccc tggtcatcat ccacaatgtt
    21841 attatttagt gttatttttt gactttccag aactaattta tagaattctt tccatttatg
    21901 ctgtgaacca acaattgcgc ccccaataat atatacatga ttgccaatca tgaaatcaaa
    21961 aacctgttgt tgagatgtga cagtaagccc tttttttata gtgaaaagat acatcttact
    22021 ttcatcaaat gggaaatccc aaattttcaa tcctcgagtt acatttggct tacggcaata
    22081 gccaaaatct atccacgcaa caagaggcgt tttcactaag cccatgttga tagctttatt
    22141 tacaaaatag gccttaaggt tacataccaa aacatactct ggtgaccagt actctgggtt
    22201 ttttaattgt cgaggctcaa gtctatttgt aaacgactca tctttttgaa tt
//
LOCUS       DAZKIV010000071        21998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZKIV010000071 DAZKIV010000000
VERSION     DAZKIV010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.071"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            247..399
                     /gene="mokC"
                     /locus_tag="ACHOT6_002639"
     CDS             247..399
                     /gene="mokC"
                     /locus_tag="ACHOT6_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFP9947832.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(503..1633)
                     /gene="dnaJ"
                     /locus_tag="ACHOT6_002640"
     CDS             complement(503..1633)
                     /gene="dnaJ"
                     /locus_tag="ACHOT6_002640"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFP9947833.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1722..3638)
                     /gene="dnaK"
                     /locus_tag="ACHOT6_002641"
     CDS             complement(1722..3638)
                     /gene="dnaK"
                     /locus_tag="ACHOT6_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFP9947834.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            4015..4419
                     /gene="yaaI"
                     /locus_tag="ACHOT6_002642"
     CDS             4015..4419
                     /gene="yaaI"
                     /locus_tag="ACHOT6_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFP9947835.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4445..5158
                     /gene="msyB"
                     /locus_tag="ACHOT6_002643"
     CDS             4445..5158
                     /gene="msyB"
                     /locus_tag="ACHOT6_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9947836.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5307..5873
                     /gene="satP"
                     /locus_tag="ACHOT6_002644"
     CDS             5307..5873
                     /gene="satP"
                     /locus_tag="ACHOT6_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFP9947837.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5908..6495)
                     /gene="mog"
                     /locus_tag="ACHOT6_002645"
     CDS             complement(5908..6495)
                     /gene="mog"
                     /locus_tag="ACHOT6_002645"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFP9947838.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6610..7563)
                     /gene="tal"
                     /locus_tag="ACHOT6_002646"
     CDS             complement(6610..7563)
                     /gene="tal"
                     /locus_tag="ACHOT6_002646"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFP9947839.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7842..9272
                     /gene="yaaJ"
                     /locus_tag="ACHOT6_002647"
     CDS             7842..9272
                     /gene="yaaJ"
                     /locus_tag="ACHOT6_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFP9947840.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9342..10118
                     /gene="yaaA"
                     /locus_tag="ACHOT6_002648"
     CDS             9342..10118
                     /gene="yaaA"
                     /locus_tag="ACHOT6_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFP9947841.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10271..10567)
                     /gene="yaaX"
                     /locus_tag="ACHOT6_002649"
     CDS             complement(10271..10567)
                     /gene="yaaX"
                     /locus_tag="ACHOT6_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFP9947842.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWPPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10781..12067)
                     /gene="thrC"
                     /locus_tag="ACHOT6_002650"
     CDS             complement(10781..12067)
                     /gene="thrC"
                     /locus_tag="ACHOT6_002650"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFP9947843.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(12068..13000)
                     /gene="thrB"
                     /locus_tag="ACHOT6_002651"
     CDS             complement(12068..13000)
                     /gene="thrB"
                     /locus_tag="ACHOT6_002651"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFP9947844.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(13002..15464)
                     /gene="thrA"
                     /locus_tag="ACHOT6_002652"
     CDS             complement(13002..15464)
                     /gene="thrA"
                     /locus_tag="ACHOT6_002652"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFP9947845.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15490..15609)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15545..15610)
                     /gene="thrL"
                     /locus_tag="ACHOT6_002653"
     CDS             complement(15545..15610)
                     /gene="thrL"
                     /locus_tag="ACHOT6_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFP9947846.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15824..16510)
                     /gene="yjtD"
                     /locus_tag="ACHOT6_002654"
     CDS             complement(15824..16510)
                     /gene="yjtD"
                     /locus_tag="ACHOT6_002654"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9947847.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(16910..17050)
                     /gene="yjjY"
                     /locus_tag="ACHOT6_002655"
     CDS             complement(16910..17050)
                     /gene="yjjY"
                     /locus_tag="ACHOT6_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFP9947848.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            17146..17862
                     /gene="arcA"
                     /locus_tag="ACHOT6_002656"
     CDS             17146..17862
                     /gene="arcA"
                     /locus_tag="ACHOT6_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFP9947849.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(17922..19274)
                     /gene="creD"
                     /locus_tag="ACHOT6_002657"
     CDS             complement(17922..19274)
                     /gene="creD"
                     /locus_tag="ACHOT6_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFP9947850.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(19332..20756)
                     /gene="creC"
                     /locus_tag="ACHOT6_002658"
     CDS             complement(19332..20756)
                     /gene="creC"
                     /locus_tag="ACHOT6_002658"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFP9947851.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(20756..21445)
                     /gene="creB"
                     /locus_tag="ACHOT6_002659"
     CDS             complement(20756..21445)
                     /gene="creB"
                     /locus_tag="ACHOT6_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFP9947852.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(21458..21931)
                     /gene="creA"
                     /locus_tag="ACHOT6_002660"
     CDS             complement(21458..21931)
                     /gene="creA"
                     /locus_tag="ACHOT6_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFP9947853.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
BASE COUNT         5260 a         5858 c         5374 g         5506 t
ORIGIN      
        1 taaatcatta aaaaattacg ccacaaaaat gcgaggcgtc ttcaggatta gaatcgataa
       61 aacagaatgg gttaaagaga gcgatataat agcggcgggt gcttgaggct gtctgtctca
      121 ggcattagct gaacggcaga tagagaaaag ccccgagtga tattttacca tcaacccgag
      181 gccccctata tgctgaacac atgtagagtg cctcttactg accgtaaggt caaggagaag
      241 agagcaatga agcagcataa ggcgatgatt gtcgccctga tcgtcatctg tatcaccgcc
      301 gtagtggcgg cgctggtaac gagaaaagac ctctgtgagg ttcacatccg aactggccag
      361 acggaggttg ctgttttcac ggcttacgaa tccgagtaag agcaacggcg gggagtgatc
      421 cccgccactt tacaggtgct cgcatatctt caacgcaccc tatttttacc caagcctgcc
      481 cacgggcagg cttttgggga ggttagcggg tcaggtcgtc aaaaaacttc ttcacaccat
      541 caaagaagct ctttgagcgc gggctgttgt gctcgccggt tgggccaccg aagctttctt
      601 gcagctcttg cagcagctgt ttctgcttct cgttcaggcc taccggtgtt tcgacgacaa
      661 cgcggcacag caaatcaccc tgtgcgccac cgcggacaga cttgacgcct ttaccgcgca
      721 tacggaaaag cttaccggtc tgggtttcgc caggcacttt cagtttgacg cgaccatcaa
      781 gggtcggtac ttcgatttcg ccacccagcg ccgccatagc gaagttgatc gggacttcgc
      841 aatacaggtt gttgccttca cgctcgaaaa tcgggtgctg tttaacctga acctgaacgt
      901 acagatcgcc tgccggtgcg ccatgctcgc ccgcttcacc ttcgcccgca agacggatgc
      961 ggtctccagt gtccacccct gccgggattt taacggacag cgttttgctg cgctcaacac
     1021 gaccatgacc atgacatttg ttgcacggat ctttgatcag cgtaccgcga ccctgacagt
     1081 gtggacaggt ctgctgcacg gcaaagaaac cctgacgcat ctgcacctga ccagaaccat
     1141 gacaggtcgg acaggtctgc ggctgtgtac ctggttttgc accgctaccg tggcaaacgt
     1201 cacactcttc cagagtcgga atgcggatct ctttggtcac gccacgtaca gcttcttcga
     1261 gggtgagctc catgttatag cgtaaatcag caccgcgcgc cgcacgttga cgaccacgtc
     1321 cgccgccaaa aatatcgccg aaaacgtcac caaaaatatc gctgaagtct gcgccgccgc
     1381 caaaaccgcc gccgcccata ccaccttgct caaacgcagc atgaccatac tgatcgtatg
     1441 ccgcacgttt ttgcgagtcg gtcagaactt cataagcttc cttgatctct ttaaatttcg
     1501 cctcggcctc tttgtcaccc tggttacggt ccgggtggta tttcatggcc aggcgtttgt
     1561 aggccttttt gatttcacgc tcttccgctg ttttggaaac gcctaaaatc tcgtaataat
     1621 cttgcttagc catctttttt aaattgcccc tagatgaatg cacgggcgga gaggaaattc
     1681 cccttcgccc gtgtcagtat aattacccgt ttatagggcg attatttttt gtctttgact
     1741 tcttcaaatt cagcgtcgac aacatcgtca tctttcgcgt tgtttgcaga agcatcagca
     1801 ccggcagtct gctgctgggc atgttgctgc tgggcgattt ccatcagttt ctgggaaacc
     1861 tgtgccagtt cctgcatttt cgcttcgata gcggctttgt cttcaccttt cagagcagtt
     1921 tccagtgcag tcagtgcaga ctcgatagca gttttgtcgt cagccggcag tttgtcgcct
     1981 gcttcttcaa cctgcttacg ggtgctgtgc agcagatggt cgccctggtt gcgagtctgt
     2041 accagctctt caaacttacg gtcagcttcg gcgttagctt ctgcgtcgcg taccattttc
     2101 tggatttcat cttcgttcag accagaagaa gccttgatgg tgatcttctg ctctttaccg
     2161 ctgtttttat ctttcgcgga aacgtgcagg ataccgtcag catcgatatc gaaggtaact
     2221 tcgatctgcg gcatgccgcg cggtgccggg ttgataccat ccaggttgaa ctgacccaga
     2281 gatttgttat cagccgcacg tttacgttca ccctgcagca catggatggt taccgcagac
     2341 tggttgtctt cagcggtaga gaacacctgg ctgtgcttgg tcgggatagt ggtgtttttc
     2401 gcgatcagcg tcgtcatcac accgcccatg gtttcgatac ccagagacag cggggtaacg
     2461 tccagcagca gtacgtcttt cacgtcacca gtcagaacac caccctgaac agcagcaccg
     2521 attgctacag cttcgtccgg gttaacgtct ttacgcggct ctttaccaaa gaactcagca
     2581 actttcttct gaaccattgg catacgagtc tgaccaccaa cgaggataac gtcgtcgata
     2641 tcagatacgg acaggccagc gtcctgcagt gcaactttca gcggctcaat ggaacggttt
     2701 accagatctt caaccaggct ttccagtttc gcacgagtca ctttgatgtt catgtgtttc
     2761 ggaccggtcg catctgcagt gatgtacggc aggttaacgt cggtctgctg agcggaagac
     2821 agttcgattt tcgctttttc tgccgcttct ttcaggcgct gcattgccag cggatcgttg
     2881 cgcaggtcaa tgccctgatc tttcttgaat tcttcaacca ggtagttgat cagacggctg
     2941 tcgaagtctt caccacccag gtgggtatca ccgttggttg ccagaacttc gaaggttttt
     3001 tcgccgtcaa cttcgtcgat ttcgataata gaaatatcga aagtaccacc acccaggtca
     3061 taaaccgcga tagtacggtt gccagtacct ttgtccagac cgtaagccag cgcagctgcg
     3121 gtcggttcgt tgatgatacg ttttacttcc agaccagcga tacggcctgc gtctttggtt
     3181 gcctgacgct gagcatcgtt aaagtatgcc ggtacggtga taacagcttc agttaccggt
     3241 tcacccaggt aatcttcagc ggttttcttc atttttttca gcacttcagc agagatctgc
     3301 ggcggtgcca ttttctggcc tttaacttcg acccatgcgt cgccgttatc agcagcaata
     3361 attttgaacg gcatgatgga aacatcacgc tgtacttctt cgtcctggaa gcggcggcca
     3421 atcaggcgtt taatcgcaaa cagggtgttt tgcgggttcg tcactgcctg acgtttagcc
     3481 ggctgaccaa ccagagtttc accatcctgg gtataggcaa tgatagaagg cgtggtgcga
     3541 tcgccttcgg cgttctccag cacacgagga gtggtgccat ccataatcgc tacacaagag
     3601 ttggtagtac ccaggtcgat accaattatt ttacccatct aaacgtctcc actatatatt
     3661 cggtcatcat gtggttgtga gtctgtaata ggggcgaaac gtctggtttc aactgcccaa
     3721 tttcattttt ttgcagactc actcactgcg gttgactact aaatggggtc gtaacccacg
     3781 tcatcaaggg ggagatgcaa aaattttttg tgcttttgcg cagaaatttc gcgatatggc
     3841 aggacaagac gtaaaattga cagcaatttt attcagataa agaatgaatc gcagccaagg
     3901 cattcatcaa aaaattgtaa taaaaagaaa agattacgta cctgaacctt ctctttatca
     3961 gcagtaaact agtgggtatt catccctcta cctcttccca ctaagagaat ccttatgaaa
     4021 tccgttttta cgatttccgc cagcctggcg attagcctga tgctgtgctg cacggcgcag
     4081 gcaaacgacc ataaactcct cggcgtcatt gcaatgccgc gtaacgaaac caacgatctg
     4141 gcgctgaaac ttcctgtttg tcgcattgtg aaacgcatac aactctctgc cgaccatggc
     4201 gatttacagt taagcggtgc atcggtttat ttcaaagccg cccgtagcgc cagtcagagt
     4261 ctgaatattc cttcagaaat aaaagaaggg caaaccactg actggatcaa cattaacagt
     4321 gataacgaca ataaacgctg cgtctcaaaa atcacctttt ccggtcatac ggtgaactca
     4381 tcggatatgg ccacgctgaa aattatcggc gacgactaac gccagaatat agaaaccaca
     4441 aaaaatgaat gttaattacc tgaatgattc agatctggat tttcttcagc attgtagtga
     4501 ggaacagttg gcaaatttcg cccgattgct cacccataat gaaaaaggca aaactcgcct
     4561 ctccagcgta ctgatgcgca acgaactgtt taaatcgatg gaagggcatc ccgagcaaca
     4621 tcgccgcaac tggcagctga ttgctggaga attacagcat tttggtggcg atagtatcgc
     4681 caacaaactg cgcggacacg gtaaattgta tcgggccatt ttgctcgatg tttcaaaacg
     4741 attgaagctg aaagccgaca aagagatgtc tacgtttgag attgagcaac agttactgga
     4801 acaatttctg cgtaacacct ggaagaatat ggacgaggaa cataagcagg agtttctgca
     4861 cgcggtcgat gccagggtga atgagctgga agagctgctg ccgctgctga tgaaagacaa
     4921 attattggca aaaggcgtgt cgcatttgct ttccagccaa ttgacccgca ttttacgcac
     4981 ccacgcagca atgagcgtgc ttgggcatgg tttgctgcgc ggcgcgggtc tgggaggccc
     5041 tgtgggcgca gcattaaatg gggttaaagc ggtcagcggc agcgcctatc gcgtgacaat
     5101 tccagccgta ctgcaaatcg cctgcctgcg ccggatggtt agcgccactc aggtctgaca
     5161 ccgtaaaccg gggaaaattt tcattttccc ctcaaaagat cgtagacact gccccactgg
     5221 ctgattatta tgccgcgccc tgaaaacact actgttattc agggaaatta tttcaccatt
     5281 cattcgatga tgatttttga ggaattatgg gcaacactaa gttggctaat ccggcaccgc
     5341 tgggcctgat gggcttcggc atgaccacca ttctgcttaa cctgcacaac gtgggttatt
     5401 tcgctctgga cggtattatt cttgccatgg gcattttcta cggcggcatc gcgcaaattt
     5461 ttgccggtct gctggagtac aaaaaaggca acactttcgg tttaaccgca ttcacctctt
     5521 acggttcttt ctggctgacg ctggttgcga ttctgctgat gccgaaactg ggtctgaccg
     5581 atgcgccaaa tgcacagttc cttggtgtct acctgggtct gtggggcgta tttacgctgt
     5641 ttatgttctt cggcacgctg aaaggcgcac gcgttctgca attcgttttc tttagcctga
     5701 ctgtgctgtt tgccctgctg gcgatcggta acattgccgg taacgccgca atcatccact
     5761 ttgccggctg gattggtctg atctgtggtg ccagtgcaat ctatctggcg atgggtgaag
     5821 tactgaacga gcagtttggt cgcaccgttc tgccgattgg tgaatcccac taattgtttt
     5881 gagatccccc cgctcggggg gattttttta ttcgctaacg tcgcgtcttg cactcttcgg
     5941 tctgaatgca gcaaccactt ctggtgccgt ttcaacgtat ggcccttcca gcaactgaat
     6001 gcagtacggt acgctggcaa aaataccgtg taccacaacg ttacccgcag cgtccttcac
     6061 accttccagc gtctctttaa tagacttcgg ctgaccgggt aagttaagga tcagcgcctg
     6121 tttgcgaatc acaccaacct gacgcgaaag gatcgcagtt ggtacaaaat gcaggcttat
     6181 ctggcgcatc tgttcaccaa agccaggcat ctcgcggtcc gctactgcca gcgtcgcatc
     6241 gggcgttacg tcacgacgcg ccgggccagt tccgcccgtg gtgagcacca gatggcaact
     6301 catttcatcc actagctcac acaacgtttg ctcgatgatc gcctgctcat cggggattaa
     6361 gcgggtttcc agttcaaacg gcgtggttag cgccgatgtc agccattctt ccagcgcagg
     6421 gatgccttta tcctgataaa cgccgctgga tgcgcgatca gagatggaaa ctaagccaat
     6481 acgtaaagta ttcatcaaga tttttccgtt taaacagtct cgttaaacag aatgatacac
     6541 tgcgaaggga gtgacagaca ggcttcagaa gaggtagcgt gaccgacttc ccggtcacgc
     6601 taagaatgat tacagcagat cgccgatcat tttttccagt ttttcctggt caatagcaaa
     6661 cttacggata ccttccgcca gtttatctac tgccattgga tcctggttgt gctgccacag
     6721 gaactcggac tcagtgatac gcgccggacg cgctttcact tcgccggtgt aagacagttt
     6781 acgttcgata gccccttcgc tctcagccag ctctttcagc agtgtaggtg cgatggtaag
     6841 acggtcgcag cctgccagtt ccagaatttc gccgatgtta cggaagcttg cgcccataac
     6901 cacggtttca taaccgtgct ctttgtagta ctggtagatt tcagatacag aaaccacgcc
     6961 cggatcttct gccggagcgt actctttctt atcggtattc gctttgtacc agtcaagaat
     7021 acggccaaca aacggcgaga tcaggaacac gcccgcttcc gcacaagcac gagcctgagc
     7081 gaaggagaac agcagggtca ggttacagtt gatgccttct ttttccagct gttctgcagc
     7141 acggataccc tgccaggtag aagccagttt gatcagaata cgatcgttgc taataccagc
     7201 atcgttgtag agtttgatca ggcgttttgc tttcgcaatt gacgcttcgg tgtcatagga
     7261 aagacgcgca tcaacttcag ttgagatacg gcccggaacc agtttcagga tttccagacc
     7321 aatatttact gccagtttgt cggtcgcgtc cacgatctgc tgcgcgcgat cgttgctctg
     7381 ctgtttcgcc caggcgacag catcatcaat caacttacgg tattccggaa tctgcgctgc
     7441 gttaagaatg agagaagggt tggttgtggc atcctgcggt tgatacagct tcattgccgc
     7501 gatgtcccca gtgtcggcca ctacggtggt gtactgacga agggaggtca atttgtccgt
     7561 catgatagta tttctcttta aacagcttgt tagggggatg taaccggtct gccctgatga
     7621 tatcacgacg caacgtcagc gcaaccgccg acaagacggt tatcgccagg cgagactgtt
     7681 tcggatttct gacactgcgt tgatatcgcc cgccattttt atacaaaacc tcatgtatgc
     7741 tacgcagaag ttatcaagta cctcgtagcg tatatacttc ttaaacaatt ggtaacgttt
     7801 acacaggaaa gtcatcgcga ccggcaataa gagggatatg catgccagat tttttctcct
     7861 ttattaacag cgtcctttgg ggatcggtaa tgatttacct gctcttcggc gcaggttgtt
     7921 ggttcacttt tcgcaccgga tttgtgcagt ttcgctacat ccgccagttt ggcaaaagtc
     7981 ttaaaaatag cattcatcca cagccaggcg gtttaacctc atttcagtca ttgtgtacca
     8041 gtcttgcggc gcgcgtgggt agcggcaacc tggccggcgt tgcgctggct attaccgccg
     8101 gtggacctgg agccgtcttt tggatgtggg ttgccgcgtt tatcggcatg gcgacctcgt
     8161 ttgccgaatg ttcccttgca caactttata aagaacgtga cgtcaatgga cagtttcgtg
     8221 gaggaccggc atggtatatg gcgcgcgggc tggggatgcg ttggatgggc gttctgttcg
     8281 ccgtcttttt gctcatcgcc tacggcataa ttttcagcgg agttcaggcg aacgccgttg
     8341 cccgagccct gagtttttct tttgattttc ccccgctggt gacaggcatt attctcgctg
     8401 tctttgctct gctggcaatc actcgcggtc ttcatggcgt cgcccggctc atgcaggggt
     8461 ttgtcccgtt gatggcgata atctgggtgc tgaccagcat ggtgatttgc gtaataaata
     8521 tcgggcaact tccccacgtc atttggtcta tttttgagag tgcttttggc tggcaggaag
     8581 cggcaggcgg cgcggcggga tataccttaa gccaggcgat tactaacggt tttcagcgca
     8641 gtatgttttc caatgaggcg ggaatgggtt cgacgccaaa cgcggcagcg gcagcggcgt
     8701 cctggcctcc gcatccggca gcgcaaggga ttgtccagat gattggcatt tttatcgaca
     8761 ccctggtcat ctgtacggca agcgccatgc tgatattact ggcgggtaac gacacaacct
     8821 acatgccgct ggaaggtatt cagcttatcc agaaggcgat gcgggtgcta atgggttcct
     8881 ggggtgctga gtttgttacc ctcgtggtta ttctgtttgc cttcagctcc atcgttgcca
     8941 actacattta tgccgaaaac aatctcttct ttttacgcct gaacaaccct aaagcgatct
     9001 ggtgtttgcg gatctgcacc ttcgcaacgg tcatcggcgg caccttgcta agccttccgc
     9061 tgatgtggca actggcagat atcataatgg cctgcatggc tattaccaat ttgaccgcca
     9121 ttttactgct ctcgcctgtg gttcatacca ttgccagtga ttatctacgc cagcgtaaac
     9181 tcggcgtgcg cccggtgttt gatccgttgc gttatccgga tatcggtcgc cagctttctc
     9241 cggacgcgtg ggatgatgtt tcgcaggagt aatcacaact atcgatcaac tcattctctt
     9301 tttttgctaa agtcggcata aatttcctgc aaggactgga tatgctgatt cttatttcac
     9361 ctgcgaaaac gcttgattac caaagcccgt tgaccaccac gcgctatacg ctgccggagc
     9421 tgttagacaa ttcccagcag ttgatccatg aagcgcggaa actgacgcct ccgcagatta
     9481 gcacgctgat gcgcatcagc gacaaactgg cgggtatcaa cgccgctcgc tttcatgact
     9541 ggcagccaga tttcacgccg gagaatgccc gccaggcgat tctggcgttt aaaggtgatg
     9601 tctacaccgg cttgcaggcc gaaaccttca gcgaagacga tttcgatttt gcccaacagc
     9661 atttgcgaat gctttccggc ttgtatggcg tactgcgccc gctcgattta atgcagcctt
     9721 atcgtctgga aatggggatc cgtcttgaga atgcccgagg gaaagatctg tatcaattct
     9781 ggggagatat catcaccaac aagctgaacg aggcgctcgc agcacaaggc gataatgtgg
     9841 tgattaatct ggcgtcagat gagtatttta aatcggtgaa gccgaagaaa ttgaatgccg
     9901 agattatcaa gccggtgttc ctcgatgaga agaacggcaa atttaagata atcagcttct
     9961 acgctaagaa agcacgcggt ctgatgagtc gttttattat tgaaaatcgg ctgaccaaac
    10021 cagagcaact gactggtttt aatagcgaag gttacttctt tgatgaagat tcctccagca
    10081 atggcgaact ggtgtttaaa cgctacgagc agcgttaagt ttgtagtcaa tgccggatgc
    10141 ggcgtgaacg ccttatccgg cctacaaaag catgcagatt caatatattg cagaattcac
    10201 gtaggcctga taagacgcgc cagcgtcgca tcaggcgctg aatgccggat tgttacccgg
    10261 catttgtcat ttagcggtga tgtttgcctg gaccatgacc gccgtgatga tcatgaggag
    10321 ctttcttatg gtggcgcggc ggtggcggcg gtccgtgtgg gggccagcga ttgcctcgcc
    10381 attcataatg ttgtttccac cagccgtggt cgcgccagtg acctccatcc cagtaatagc
    10441 cacgattatc acgatcgcct atctgtaatt ttactgacgg gactaacgta atttccgcag
    10501 cctgtgctgc catgggagcg accagaacca gggaaagtgc gagtacgata gattgcatct
    10561 ttttcacagg ttactccttt tatcattgcc cgttatgggt cgatatccgg agattacgcg
    10621 atgagaacga cggttgttat tctccgcaat cctaatctct aagttaccgc atttattgag
    10681 aatttctcct ttttccctgt cgtttttctc cataatttct tcataaaaaa gccgggctgc
    10741 ataaaagcaa acccggcctg attgagataa tgaatagatt ttactgatga ttcatcatca
    10801 atttacgcaa cgcagcaaaa tcggcgggca gattatgtga aagcaagggt aaatcagcac
    10861 gttctgccag ctcttttggc agatccaacg tttcaccgag aatcgcttcc acgctctctt
    10921 taaatttcgc cggatgcgcg gtgccgagga acaagccata ttcgcctgga ttcaactggt
    10981 cacgcagcgc acgatacgct acggcagcgt gcggctccga ggtgtagccc agttctttta
    11041 actcacgcat tgtctgttgc gtggtttcat catccacggc tgcataaccc agctctttca
    11101 gttgccagat tttgcggcgg aacaactctt ccacacgcgg ccagttgttc ggctgactca
    11161 cgtccatcgc gttggataac gtcgcctgag tcgctttggg tgaccactga ccgtcgtgca
    11221 ggaaacgtgg cacggtatcg ttcacgttgg tcgcagcaat aaaacgtttc accggcagac
    11281 cgagtgactt cgccagcaga cccgccgtca aatcgccgaa gtttccgctt ggcaccgaga
    11341 caaccagctg gttgcgcgct tcctgcggca gctgcgcaac agcttcaaag tagtagcaaa
    11401 tctgcgccag caaacggctg atgttaatcg agttagctga gtttaacccc agcgccactt
    11461 tcagttcttc atcatcaaac gcctgcttca ccagcgcctg acaggcatcg aaatcgccgt
    11521 cgatggcaac agtttcgata ttgccgccca acgtacagaa cagtttttct tgcagtggac
    11581 tgattttgcc tcgtggatag aggataacca ctttcacatt cggtaaaccg tagaaagcat
    11641 gagccactgc cgctccggta tcaccggagg tcgcggtcag aatggtcact ggcttatcgc
    11701 ccgcaatatg ggtcagcatt tgtgccataa agcgaccgcc gaaatcttta aatgccagcg
    11761 ttggcccgtg gaacaattcc agacaaccga catcgctttc aacattggcg accggagccg
    11821 ggaaggcaaa cgccgcgcgc acgcgctctt ccaggatttc ctgcgggatt tcatcaccaa
    11881 taaacgccga gaggatcttc gcactgcggg tgacaaaatc cagcttcagc atctcatcaa
    11941 tttcagtcag gctgaattcc ggcaggtcat gcggaaaaaa cagcccctga tttttgccca
    12001 acccctgggt tacggcttgc gcaaagctga cctgctcgtt gtgatctttc agattgtaga
    12061 gtttcattta gttttccagt actcgtgcgc ccgccgtatc cagccggcaa atatgaacaa
    12121 aaccttcctg attttgcagg tagttcttac ccaaccagtc ggcaacgcgc tgggcggtat
    12181 ccggcttgtc acatagagcg aacaaggtcg ggccggagcc ggagataccg ctcgctaccg
    12241 cgccgatttc cgcgaccgcc tgccgcgcct gccggaagcc aggcagtaac cgttcacggt
    12301 agggttcagc gataacatct ttcatcagct tcgcggcaag ctcaggctga cgggaatagc
    12361 aggcgtgaat gaagccagcc agatgtcgcc cgtgcgcaat gcaatcctgg cggcgatact
    12421 gcgccggtaa aatagccctg gcttctgccg tcgagacttt aatccccgga tacgccagca
    12481 cccacagcca ctcatcaaac cctggcactt gctggctgat gatgtcgttt tcttcgatca
    12541 tcaactgcat accaccaaga aaacacggtg ccacgttgtc gtaatgaatg ctgccggaga
    12601 ttcgtccttc cagctcgccc atcaaagcca gcaaacgagt gtcattaagc ggcttgccac
    12661 agtgttcatt catcgccatc agcgccgcga ccaccgaaca ggcgctggag cctaagcccg
    12721 aaccgattgg catattcttt tccagagtca tcgccactgg aatttgcttg ccaagctcct
    12781 ggcaaaaacg ctcccagcac tgataaacga tattttcccg tggttctgac ggcagcttat
    12841 cggcaaagcg tccgaggttg ttgagactga atgtctctgc cgcctcaacc gtgactacat
    12901 ctccgagcaa tgcaccatca acaggtgtca ccgccgcccc gagcacatca aacccgacgc
    12961 tcatattggc actggaagcc ggggcataaa ctttaaccat gtcagactcc taacttccat
    13021 gagagggtac gtagcagatc ggcaaagaca ccggcagctg taacgtcatt gcccgcacca
    13081 tatccgcgca gcaccaacgg cagcggctga taatagtggc tataaaaggc cagggcgttt
    13141 tcgccatttt tcactttgaa cagcggatca ttaccatcca cttcggcaat cttcacgcgg
    13201 caggcaccat cttcatcaat attgccaaca tagcgcaaaa cttttccttc atcacgggcc
    13261 ttcgccacgc gcgcggcaaa gagatcgtcg agctgtgaca gattcgccat aaaagcggca
    13321 acatcaccct cagcgttaaa ctctgcgggc agcacaggtt caatttcaat atccgccagc
    13381 tccagttcac gtcccgtttc acgagcgaga atcaatagct tacgcgctac atccatacca
    13441 gaaagatcat ctcgcggatc cggttcggta taacccattt cccgcgccag agtagtcgcc
    13501 tcggagaaac tcatgccttc gtctaacttg ccgaagatat aagaaagcga acctgaaaga
    13561 atgccggaga acttcatcaa ttcatcacca gcattgagca gattttgcag gttctcaata
    13621 accggtaatc cagccccaac gttggtgtca tagaggaatt tacgccgcga tttttccgcc
    13681 gcatgacgca acagatggta gtaatccatc gacgaggtgt tggccttttt gttcggcgtg
    13741 acaacgtgga aaccttcgcg caagaagtcg gcatattgat ccgccactgc ctggctggag
    13801 gtgcagtcaa caatgaccgg gttcagcaga tgatattctt tcacgaggcg aattaagcgc
    13861 ccgagattaa acggctcttt ggcttgcgcc agttcttcct gccagttttc cagatttagg
    13921 ccatgcacat tggtgagcag tgccttcgag ttggcaacac cgcagacacg taagtcgata
    13981 tgtttattct tcagccagct ttgttgacgc ttcagttgct ccagcagcgc accgccaacg
    14041 ccaccgacgc caatcacaaa cacttcgata acctgatcgg tattgaacag catctgatga
    14101 gtaacgcgca cgccagtggt cgcatcatcg ttatttacca cgacagagat tgagcgttca
    14161 gaagatccct gagcaatggc gacaatgttg atattggcgc gggccagcgc ggcaaagaat
    14221 ttcgccgaga tcccacgcaa ggtgcgcata ccatcaccta ccaccgagat aatggccagc
    14281 cgttccgtca ccgccagcgg ctccagtaag ccttctttca gttccaggta gaactcttcc
    14341 tgcattgccc gttcagctcg cacacagtcg ctttgtggaa cgcagaaact gatactgtat
    14401 tcggaagatg attgcgtaat cagcaccacg gaaatacggg cgcgtgacat cgcggcaaag
    14461 acgcgcgccg ccatgccgac cattcctttc atccccgggc cggaaacgct gaacattgcc
    14521 atgttattca gattggaaat gcccttgacc ggtaattcgt cttcatcacg actggcacca
    14581 atgagcgtac ctggtgcttg aggatttccg gtatttttaa tcaggcaagg gatctggaac
    14641 tgggcgatgg gggtaatggt gcgggggtga agaactttag cgccgaagta ggaaagctcc
    14701 atcgcttcct ggtaggacat cgacttcaac aacctcgcat cgggcacctg acgcgggtcg
    14761 caggtataga ccccgtcaac gtccgtccaa atctcgcaac aatcggcgcg taaacaggca
    14821 gccagcaccg ccgcggagta gtcggaaccg ttgcgtccaa gtaccaccag ttcgcctttt
    14881 tcattaccgg cggtgaaacc tgccatcaac accatgtgat cagccggaat gcggcttgcc
    14941 gcaatacggc gggtggactc agcaatatcg acggtagatt cgaggtaatg ccccactgcc
    15001 agcagttttt cgaccggatc gataacggta acgttgtgac cacgcgcttc taacacgccg
    15061 gccataatgg cgatcgacat tttctcgcca cggcaaatca gcgcagcgtt gatgctatcc
    15121 gggcactgcc ccaacaaact aatgccatgc aggacatgtt ttatttgggc aaattcctga
    15181 tcgacgaaag ttttcaattg cgccagcggg aaccccggct gggcggcggc gagtcccgtc
    15241 aaaagttcgg caaaaatacg ttcggcatcg ctgatattgg gtaaagcatc ctggccgcta
    15301 atggtttttt caatcatcgc caccaggtgg ttggtgattt tggcgggggc agagaggacg
    15361 gtggccacct gcccctgcct ggcattgctt tccagaatat cggcaacacg cagaaaacgt
    15421 tctgcatttg ccactgatgt accgccgaac ttcaacactc gcatggttgt tacctcgtta
    15481 cctttggtcg aaaaaaaagc ccgcactgtc aggtgcgggc ttttttctgt gtttcctgta
    15541 cgcgtcagcc cgcaccgtta cctgtggtaa tggtgatggt ggtggtaatg gtggtgctaa
    15601 tgcgtttcat ggatgttgtg tactctgtaa tttttatctg tctgtgcgct atgcctatat
    15661 tggttaaagt atttagtgac ctaagtcaat aaaattttaa tttactcacg gcaggtaacc
    15721 agttcagaag ctgctatcag acactctttt tttaatccac acagagacat attgcccgtt
    15781 gcagtcagaa tgaaaagctg aaaaatactt actaaggcgt tttttatttg gtgatatttt
    15841 tttcaatatc atgcagcaaa cggtgcaaca ttgccgtgtc tcgttgctct aaaagcccca
    15901 ggcgttgttg taaccagtcg accagtttta tgtcatctgc cactgccaga gtcgtcagca
    15961 atgccatgac tcgttcgcgt aaagcttgca gttgatgttg gtctgccgtt gtgtcacttt
    16021 tcgccggttg ttgtattaat gttgctaatt gatagcaata gaccatcacc gcctgcccca
    16081 gattgagcga aggataatcc gccaccatcg gcacaccagt aagaacgtca gccaacgcta
    16141 actcttcgtt agtcagcccg gaatcttcgc gaccaaacac cagcgcggca tggctcatcc
    16201 atgaagattt ttcctctaac agcggcacca gttctactgg cgtggcgtag taatgatatt
    16261 tcgcccgact gcgcgcagtg gtggcgacag tgaaatcgac atcgtgtaac gattcagcca
    16321 gcgtcgggaa aactttaata ttatcaataa tatcaccaga tccatgtgcg acccagcggg
    16381 tggctggctc caggtgcgct tgactatcga caatccgcag ttcgctaaaa cccatcgttt
    16441 tcattgcccg cgccgctgcc ccaatatttt ctgctctggc gggtgcgacc agaataatcg
    16501 ttatacgcat attgccactc ttcttgacca aataaccgcg aacagggtga tcactgccaa
    16561 cttattacgc ggtgcgaatt tacaaattct taacgtaagt cgcagaaaaa gccctttact
    16621 tagcttaaaa aaggctaaac tatttcctga ctgtactaac ggttgaattg ttaaaaaatg
    16681 ctacatatcc ttctgtttac ttaggataat tttataaaaa ataaatctcg acaattggat
    16741 tcaccacgtt tattagttgt atgatgcaac tagttggatt attaaaataa tgtgacgaaa
    16801 gctagcattt agatacgatg atttcatcaa actgttaacg tgctacaatt gaacttgata
    16861 tatgtcaacg aagcgtagtt ttattgggtg tccggcccct cttagcctgt tatgttgctg
    16921 ttaaaatggt taggatgaca gccgtttttg acactgtcgg gtcctgaggg aaagtaccca
    16981 cgaccaagct aatgatgttg ttgacgttga tggaaagtgc atcaagaacg caattacgta
    17041 ctttagtcat gttacgccga tcatgttaat ttgcagcatg catcaggcag gtcagggact
    17101 tttgtacttc ctgtttcgat ttagttggca atttaggtag caaacatgca gaccccgcac
    17161 attcttatcg ttgaagacga gttggtaaca cgcaacacgt tgaaaagtat tttcgaagcg
    17221 gaaggctatg atgttttcga agcgacagat ggcgcggaaa tgcatcagat cctctctgaa
    17281 tatgacatca acctggtgat catggatatc aatctgccgg gtaagaacgg tcttctgtta
    17341 gcgcgtgaac tgcgcgagca ggcgaatgtt gcgttgatgt tcctgactgg ccgtgacaac
    17401 gaagtcgata aaattctcgg cctcgaaatc ggtgcagatg actacatcac caaaccgttc
    17461 aacccgcgtg aactgacgat tcgtgcacgc aacctgctgt cccgtaccat gaatctgggt
    17521 actgtcagcg aagaacgtcg tagcgttgaa agctacaagt tcaatggttg ggaactggac
    17581 atcaacagcc gttcgttgat cggccctgat ggcgagcagt acaagctgcc gcgcagcgag
    17641 ttccgcgcca tgcttcactt ctgtgaaaac ccaggcaaaa ttcagtctcg tgctgaactg
    17701 ctgaagaaaa tgaccggccg tgagctgaaa ccacacgacc gtactgtaga cgtgacgatc
    17761 cgccgtattc gtaaacattt cgaatctacg ccggatacgc cggaaatcat cgccaccatc
    17821 cacggtgaag gttatcgctt ctgtggtgat ctggaagatt aatcggcttt accaccgtca
    17881 aaaaaaacgg cgctttttag cgccgttttt atttttcaac cttatttcca gatacgtaat
    17941 tgatcgtcca ttgtaacttc tttactggct ttcattttcg gcagtgaaaa cgcataccag
    18001 tcgatattac gagtcacaaa catcatgccg gccagcgcca ccaccagcac actggttccc
    18061 aacaacagcg cgctatcggc agagttgagc agtccccaca tcacaccatc cagcaacaac
    18121 agcgcgaggg taaacaacat gctgttgcgc cagcctttca ataccgcctg caaataaata
    18181 ccgttcatta acgccccaat cagactggcg attatccatg ccacggtaaa accgatatgt
    18241 tcagaaagcg ccagcaagag caaataaaac atcaccaatg aaagccccac cagcaaatat
    18301 tgcattgggt gtaaacgttg cgcggtgagc gtttcaaaaa caaagaacgc cataaaagtc
    18361 agtgcaatca gcagaatggc gtacttagtc gcccggtccg ttaattggta ttgatcggct
    18421 ggcgtcgtca ctgcgacgct aaacgcgggg aagttttccc agccggtatc attgcctgaa
    18481 gcaaaacgct caccgagatt attagcaaac cagctgcttt gccattgcgc ctgaaaacct
    18541 gactcgctaa cttcccgttt ggctggcaaa aaatcaccta aaaaactggg atgcggccag
    18601 ttgctggtta aggtcatttc gctattacgc ccggcaggca ccacagaaag atcgccggta
    18661 ccgcttaaat tcagggccat attcagcttc aggttctgct tccgccagtc cccttcgggt
    18721 aaagggatat gcacgccctg cccgccttgc tctaacccgg tgccgggttc aatggtcagc
    18781 gccgttccgt taacttcagg cgctttcacc acaccaatac cacgcgcatc cccgacgcta
    18841 atcacaataa atggcttgcc aaaggtgata tttggcgcgt cgagttcgct aagacgcgaa
    18901 acatcgaaat cggcttttag cgttaaatca ctgtgccaga cctgaccggt ataaatccct
    18961 atcttgcgtt cttccacgtt ctgattgcca tcaaccatca atgactcagg taaccaaaaa
    19021 tggataaaac ttcgtttccg ctccacggtt ttatcctctt cctgtaccgt ataaagctcg
    19081 gtcacaggaa tagcgataag cggcccaacg agtttttgcg gcccgctggt actttggcga
    19141 atcgcatctt ccacatcgct acggtaatcg gcacgttcga caatcacctg ccgaatcagc
    19201 attatcggaa tcaacaacag caatactgca ccaaacaggg tggtcatttt ccagaacagg
    19261 ggggatttca acatagtctt ctcctttgca atggcagcag gatacgaagg ctatgtggga
    19321 agaatttgaa gctatgtgaa gtgacggtga agtcgaagcg aggccagcac gccaccttcc
    19381 tgcacgttgc gcagcgtgac ttcgccatta aacaaacggg cgacctcact gacgaacgcc
    19441 aaccccagac cgctgctttt ttgcccattt gcacgcggca aagagtaaaa gcgttcaaaa
    19501 atacgtgaca gcgcgtagtc aggaatacca ctaccggtat ccagcacctt aagcgtgacg
    19561 tgttcctgat ccacttcggc gcttagcgtt atacgaccgc tctcgggggt aaaatcgatg
    19621 gcgttatcca gtaaattccc cagcgcctga tccagtaacg ccggttcagc agcaacatta
    19681 acctcggtgg gcgtaacatg tagagtgatg tttttttctg ccaactgcac ggtgcgcgct
    19741 tcgctgacgc ggcggaataa tgccgccaca tcaacagcag tcaaaacgac ttcctgacga
    19801 ttctccagtc ttgcctggcg tagtaacgtt tccaccagtg cctgcattcg cgcattttgc
    19861 gtcagaatgt tgtcagtaaa acgagccact acttcaggcg gcggaccttc gcgtaaaatt
    19921 tccgccgcgc cacgaatcgc cgccagtggg ctttttagct catgggttaa cgcataaaca
    19981 tactgctcaa tatagttttt cccttccagc ttcacgcgca tactttccag cgcctgcgcg
    20041 agtttacgca gctcgctact accgagatcg ggaagaggaa cgggcttatt gtcagtgacg
    20101 gaatcagcat agcgagtgag ccgggcaatg gagcggttga tccaccaaac catgcctgcg
    20161 ccaatcacca gtgcgatccc caacaaaatg gcgctggccc ataaaattcg ccgctcgctg
    20221 cgtttaatga ccggagccat cgccgcgttc ggtttgccta cgctcaaaac gccaataagc
    20281 cgcgagccgt ccataatcgg cgcggcaaca tacatcaccg aactttcggg atcggcagga
    20341 ttttgcaacg tgctgcgcgc accatactga ccacgcaacg ttagccagac gtcattccag
    20401 cgcgaataat cctgcccaac ggctttattt gccgaatcga acaatacttt accctgcgaa
    20461 tcggtcatat agacatgata ttcattgcgc actttgttaa tgccaccgat attggcgcga
    20521 aacgggcgat gttgtagctg attaaacgcc tgcgccagtt gcccatgcgt tgggtcccca
    20581 gagagcaaat cgggacgcgc cagctccgcc agcaacgttg cggtgtcgat caacgtcccc
    20641 tccgttgctc ttcgcacgcc cggtttaact tctttgacaa aaatagccag tacgaaccag
    20701 gccgccaccg ccaccagtaa aaaatagccc agcagcaacc gcatgccgat acgcattaca
    20761 ggcccctcag gctatatccc atgccgcgat gagtattaat cggtgaaaga tcggggttga
    20821 tggcgcgcag cttggcacgc aacgttttaa tgtgggtatc aacggtgcga tcgtaggtgt
    20881 cctgcgcatc ttcccacacg ctatccatca gttgctggcg ggaccagacg cggcctggcg
    20941 acttgagtaa cgtcttcagc aataaaaact cataccgcgt cagcgttaat ggcgtgtcaa
    21001 accagctgat ctgcgccgcg ggttcattca attcaaaatg accaatacgg atgacgggag
    21061 acggtgacga gaacttcttc acccgacgca gtaaggtgcg caccctggcg cacacttcgc
    21121 ggggtgaaaa cggtttagcc acatagtcgt cagcgccaat ttccagccca agcaggcgat
    21181 cgacctcttc acttcgggcc gtcaggaaca gtacaggtaa cgccggatgg agcgccagta
    21241 actggcggca caattcaaag ccactgatat caggcaaacc aacgtcaaga atcatgacgt
    21301 cgggaacctg ctggcgagct ttatccagca ccggcaaacc tcgctcaaag acctcgacgg
    21361 caaaaccttc ctgctgcaac atgtagacca gcgtgtcggc tatcccttgc tcatcttcca
    21421 ctaaccagac cgtttcccgt tgcataaatc gcctctgtta ttgccgccac ggcatgacag
    21481 gaacagcgct aatcgcgttt ttcggcgaac cttctacaac tttgtcggag taagccagat
    21541 aagcgagcgc gttgcgtttg gcgtcataaa agcgcacgac ctgtaacgac ttaaagacca
    21601 gggacgtgcg ttttttgaat actacctcgc cctgagcttt gccgttttta atacgatccg
    21661 acagttcaat cggcccgact tgctgacaag aaatggccgc atcggaggta tcttctgcca
    21721 ggcccaatcc ccctttaata ccaccggttt tcgcccggct cacataacag gtgacatttt
    21781 tcacatcggg atcgtcaaag gcttccacaa caattttgtg atccgggccg atcattttaa
    21841 atacggtgtc gaccgaacca atctcttctg catgagccaa tggccccagc attatcaggc
    21901 ttaaagacag gatcaaatgc ttgtatttca tattgttacc atttttatct attacacgag
    21961 caattagttc gtcacggtaa ataacatatt gattaaga
//
LOCUS       DAZKIV010000072        21959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZKIV010000072 DAZKIV010000000
VERSION     DAZKIV010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.072"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            319..990
                     /locus_tag="ACHOT6_002661"
     CDS             319..990
                     /locus_tag="ACHOT6_002661"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFP9947854.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1000..2196
                     /gene="yaiP"
                     /locus_tag="ACHOT6_002662"
     CDS             1000..2196
                     /gene="yaiP"
                     /locus_tag="ACHOT6_002662"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFP9947855.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2174..2755
                     /locus_tag="ACHOT6_002663"
     CDS             2174..2755
                     /locus_tag="ACHOT6_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFP9947856.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2757..3530
                     /gene="yaiO"
                     /locus_tag="ACHOT6_002664"
     CDS             2757..3530
                     /gene="yaiO"
                     /locus_tag="ACHOT6_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFP9947857.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTDYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3715..3990
                     /gene="frmR"
                     /locus_tag="ACHOT6_002665"
     CDS             3715..3990
                     /gene="frmR"
                     /locus_tag="ACHOT6_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFP9947858.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4025..5134
                     /gene="frmA"
                     /locus_tag="ACHOT6_002666"
     CDS             4025..5134
                     /gene="frmA"
                     /locus_tag="ACHOT6_002666"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFP9947859.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHGGKSIRTVIRY"
     gene            5228..6061
                     /gene="fghA"
                     /locus_tag="ACHOT6_002667"
     CDS             5228..6061
                     /gene="fghA"
                     /locus_tag="ACHOT6_002667"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9947860.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6287..6826)
                     /gene="yaiL"
                     /locus_tag="ACHOT6_002668"
     CDS             complement(6287..6826)
                     /gene="yaiL"
                     /locus_tag="ACHOT6_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFP9947861.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6928..8139)
                     /gene="mhpT"
                     /locus_tag="ACHOT6_002669"
     CDS             complement(6928..8139)
                     /gene="mhpT"
                     /locus_tag="ACHOT6_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFP9947862.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYRS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8414..9427)
                     /gene="mhpE"
                     /locus_tag="ACHOT6_002670"
     CDS             complement(8414..9427)
                     /gene="mhpE"
                     /locus_tag="ACHOT6_002670"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFP9947863.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9424..10374)
                     /gene="mhpF"
                     /locus_tag="ACHOT6_002671"
     CDS             complement(9424..10374)
                     /gene="mhpF"
                     /locus_tag="ACHOT6_002671"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFP9947864.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEI
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10371..11180)
                     /gene="mhpD"
                     /locus_tag="ACHOT6_002672"
     CDS             complement(10371..11180)
                     /gene="mhpD"
                     /locus_tag="ACHOT6_002672"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFP9947865.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11190..12056)
                     /gene="mhpC"
                     /locus_tag="ACHOT6_002673"
     CDS             complement(11190..12056)
                     /gene="mhpC"
                     /locus_tag="ACHOT6_002673"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFP9947866.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12074..13018)
                     /gene="mhpB"
                     /locus_tag="ACHOT6_002674"
     CDS             complement(12074..13018)
                     /gene="mhpB"
                     /locus_tag="ACHOT6_002674"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFP9947867.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(13020..14684)
                     /gene="mhpA"
                     /locus_tag="ACHOT6_002675"
     CDS             complement(13020..14684)
                     /gene="mhpA"
                     /locus_tag="ACHOT6_002675"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFP9947868.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QREIVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            14875..15708
                     /gene="mhpR"
                     /locus_tag="ACHOT6_002676"
     CDS             14875..15708
                     /gene="mhpR"
                     /locus_tag="ACHOT6_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFP9947869.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQE
                     RKELIEMLAARPGDDYQLAREPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAQQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15785..16867
                     /gene="lacI"
                     /locus_tag="ACHOT6_002677"
     CDS             15785..16867
                     /gene="lacI"
                     /locus_tag="ACHOT6_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFP9947870.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16990..20064
                     /gene="lacZ"
                     /locus_tag="ACHOT6_002678"
     CDS             16990..20064
                     /gene="lacZ"
                     /locus_tag="ACHOT6_002678"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFP9947871.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPERAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCSADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20116..21369
                     /gene="lacY"
                     /locus_tag="ACHOT6_002679"
     CDS             20116..21369
                     /gene="lacY"
                     /locus_tag="ACHOT6_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFP9947872.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21435..>21959
                     /gene="lacA"
                     /locus_tag="ACHOT6_002680"
     CDS             21435..>21959
                     /gene="lacA"
                     /locus_tag="ACHOT6_002680"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFP9947873.1"
                     /translation="MNMPMTERIRAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWVEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSIVTKDIPPNVVAA"
BASE COUNT         5188 a         5915 c         5646 g         5210 t
ORIGIN      
        1 gcatattaca ttcacataat gtatataaac aatacttgtt cacagaacat attttttaaa
       61 tgcaattaat tgacaaacta aatcacatct aaatacaatg tataaaacaa aataactata
      121 cataccaaac atacaaacac ttaacatatg aattgcataa aaacattacc cagtcgaacc
      181 tccgggtctg aaagcccttg cttttcagtt attagcaaca tttaacattt ataaattaac
      241 atctggctgc gccagagatt acgtctctga gtgcatcgtt gtgcttgtag tataataaat
      301 aaaatggagt gtcattaagt ggataaggtt ttagattcag ccctcctttc ttcggcaaat
      361 aaaagaaagg gtatccttgc tattggcgca catcctgacg atatagaatt aggctgtggc
      421 gcatcgcttg ctcgtcttgc gcaaaaagga atttatatcg cagccgtggt gatgactacc
      481 ggcaactctg gtacagatgg aataatcgat cgccatgaag aatcgcgcaa cgccctaaag
      541 atattagggt gccaccaaac tattcatctt aattttgctg acacccgcgc tcatttacag
      601 ctcaatgata tgatttccgc tctggaagac atcattaaaa atcaaattcc ttctgatgtt
      661 gaaatcatgc gggtatatac catgcatgat gccgaccgcc atcaggatca tctcgctgtt
      721 tatcaagctt caatggttgc ctgccgcact attccacaaa ttctcggcta cgaaacccca
      781 agtacctggc tttcatttat gcctcaggtt tttgaatccg ttaaagaaga atatttcaca
      841 gtcaaacttg ctgcattaaa aaaacataaa agccaggaac gacgtgatta tatgcgtcat
      901 gatcgcctgc gtgcagttgc acaatttcgc gggcaacagg tcaatagcga tctgggtgaa
      961 ggctttgtaa ttcataaaat gattctttga gcaaattgca tgaaaacctg gatatttatc
     1021 tgtatgtcca tagcaatgtt gctatggttt ttaagtacgc taagacgtaa acccagtcaa
     1081 aagaaaggct gtattgacgc cattatacct gcgtataacg aaggcccgtg tctggcgcag
     1141 tcactggata atctactgcg taacccttat ttttgccggg taatttgcgt taacgacggc
     1201 tccacggaca ataccgaagc ggtcatggcg gaagtcaaac gcaaatgggg cgaccgcttt
     1261 gttgccgtca cgcaaaaaaa taccggtaaa ggtggtgcgc tgatgaatgg cctcaattat
     1321 gccacctgcg accaggtttt tttaagtgat gccgacacct atgttccgcc cgatcaagac
     1381 ggaatgggct atatgctggc agaaattgag cgcggtgctg atgccgtagg cggcatcccc
     1441 tctactgcgt tgaaaggcgc ggggctgtta ccgcacatcc gcgcgaccgt aaagttgccg
     1501 atgattgtta tgaagcgcac gctacagcag ctcctaggcg gcgcaccgtt tattatcagc
     1561 ggtgcctgcg ggatgttccg tactgatgta ttgcgtaagt tcggtttctc ggatcgtact
     1621 aaagtcgaag accttgatct cacctggaca ttagtggcaa acggctaccg tattcggcag
     1681 gcgaatcgct gcatcgtata cccacaggaa tgcaacagcc cgcgtgagga gtggcgtcgc
     1741 tggcggcgtt ggattgtggg atacgcggtc tgtatgcgcc tgcataaaag acttttattt
     1801 agccgcttcg gtatcttcag tatatttcct atgctgttgg ttgtgattta tggcgttggg
     1861 atttatctca ctacctggtt taatgaattc atcaccaccg ggccgcatgg tgtggtgttg
     1921 gcaatgtttc cgcttatctg gatcggcgta gtttgtgtta ttggtgcttt tagcgcctgg
     1981 tttcatcgtt gctggttgtt ggtgccttta gcgccgcttt ccgttgtgta tgtattatta
     2041 gcttatgcca tctggattat ttatggactt attgcctttt ttactggacg cgaacctcag
     2101 cgcgacaaac ccacccgcta ttccgcactg gtggaagcgt caaccgctta ttcccaacct
     2161 tctgtcacag gaactgaaaa actatctgaa gcttaatgtt aaagagaaaa atattcagat
     2221 tgcagaccag gtaattattg atgaaactgc aggtgaagtt gttatcggcg cgaatacccg
     2281 tatttgtcat ggtgccgtta ttcagggtcc ggtagtgatt ggcgcaaact gcctgatagg
     2341 taattatgcg tttattcgtc ctggcacaat aatcagcaat ggtgtaaaaa ttggttttgc
     2401 caccgaaatt aaaaatgcgg ttattgaagc ggaagcaacg attggtccgc aatgttttat
     2461 tgccgactcg gtagttgcaa accaggcata tttgggcgca caagtacgta ccagtaatca
     2521 tcgtctggat gaacagcctg tgtctgttcg aactccagag ggaattatcg ctaccggatg
     2581 cgataaatta ggttgttata tcgggcagcg ttcacgcctt ggcgtacaag ttataatttt
     2641 gcctgggcga attatttccc cgaacacgca acttggcccg cgcgtgattg tagaacgtaa
     2701 tttacctgcc ggaacttatt cactccgaca agaacttatc cgtacaggag attaaaatga
     2761 taaaacggac gttattagcg gcggccattt ttagcgcatt gcccgcttat gccgggttaa
     2821 cttccattac cgcgggctac gattttaccg attattctgg cgatcatggc aaccgtaatt
     2881 tagcgtatgc tgaactggtg gcgaaagttg aaaacgcaac gctgcttttt aatctttccc
     2941 aggggcgtcg tgattatgaa actgaacatt tcaatgccac tcgcggtcag ggtgcggtct
     3001 ggtataaatg gaataactgg ctgacaaccc gaacgggtat tgcctttgcg gataatacgc
     3061 cggtctttgc ccgccaggat tttcgtcagg atattaacct ggccctgttg ccaaaaacgc
     3121 ttttcacgac cggttatcgc tacactaaat attacgatga tgtcgaagtc gatgcctggc
     3181 aaggcggcgt atcactctat actggcccgg tgatcaccag ctaccgctat acccattatg
     3241 actccagcga tgcaggtggt agttatagca atatgatttc cgtgcgtctg aatgacccgc
     3301 gcggcactga ttatacgcaa ctatggctaa gccgcggaac aggtgcttac acctatgact
     3361 ggacgccaga aacacgctac ggcagcatga agagcgtcag tctgcaacgt attcaaccgc
     3421 tgactgagca acttaatctc gggctgacgg caggtaaagt gtggtacgac accccaaccg
     3481 atgattataa cggtctgcaa cttgcagccc atctgacctg gaaattctga ttccttctgc
     3541 cgcccgctat ccggggcggc cttccctgcc gattagcccc ccctttcctc tttgttttcc
     3601 gaccacattc accggataaa ttttattctc cagtgttata tactataggg gggtatgcat
     3661 tgacatatag catccccccc tatagtatat tgcgtgcaga tgatgaggtg cgaaatgccc
     3721 agtactccgg aagagaagaa aaaggtcctt actcgagttc gtcgtattcg agggcagatt
     3781 gatgctctgg aacggtcgct ggagggtgat gccgaatgcc gtgccatact ccaacagatc
     3841 gctgccgttc ggggcgcggc taacgggctg atggcagaag tgcttgaaag ccatatccgg
     3901 gaaacgtttg accgaaatga ctgctacagc cgcgaagtca gccaatccgt tgacgacact
     3961 attgaactgg ttcgagccta tcttaaatag ctgaatctat taccatattg aggaagagcg
     4021 agagatgaaa tcacgtgctg ccgttgcatt tgctcccggt aaaccgctgg aaatcgttga
     4081 aattgacgtt gcaccaccga aaaaaggtga agtgctgatt aaagtcaccc ataccggcgt
     4141 ttgccatacc gacgcattta ccctctccgg ggatgacccg gaaggtgtat tcccggtggt
     4201 tctcggtcac gaaggggccg gcgttgtggt tgaagtcggt gaaggcgtaa ccagcgtcaa
     4261 acctggcgac catgtgatcc cgctttacac cgcggagtgc ggcgagtgtg agttctgtcg
     4321 ttctggcaaa actaacctct gtgttgcggt tcgcgaaacc cagggtaaag gcttgatgcc
     4381 agacggcacc acccgttttt cttacaacgg gcagccgctt tatcactaca tgggatgctc
     4441 aacattcagt gaatacaccg tggtcgcgga agtgtctctg gcaaaaatta atccagaagc
     4501 aaaccatgaa cacgtctgcc tgctgggctg tggcgtgacc accggtattg gcgcggtaca
     4561 caacacagct aaagtccagc caggtgattc tgttgccgtg tttggtcttg gcgcgattgg
     4621 tctggcagtg gttcagggcg cgcgtcaggc gaaagcgggt cggattatcg ctattgatac
     4681 gaacccgaag aaattcgatc tggcgcgtcg cttcggtgcc accgactgca ttaacccgaa
     4741 tgactacgac aaaccgatta aagatgtcct gctggatatc aacaaatggg gtatcgacca
     4801 tacctttgaa tgcatcggta acgtcaacgt gatgcgtgcg gcgctggaaa gtgcgcaccg
     4861 cggctggggt cagtcggtga tcatcggggt cgccggttct ggtcaggaaa tctccacccg
     4921 accattccag ttggtcaccg gtcgcgtatg gaaaggttcc gcgtttggcg gcgtgaaagg
     4981 tcgttcccag ttaccgggta tggttgaaga tgcgatgaaa ggtgatatcg atctggaacc
     5041 gtttgtcacg cataccatga gcctggatga aattaatgac gccttcgacc tgatgcatgg
     5101 aggcaaatcc attcgaaccg taattcgtta ctgatttccc gcaggtttac cccgtccagt
     5161 tcaggcgggg gttcttaata ctctccccgg gcagccgtcc gggggattaa ccgtgagata
     5221 atgactgatg gaactcattg aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg
     5281 gcattattcc caatcactga aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc
     5341 cgcgaatgaa aaattgccgg tgctgtactg gctttcaggc ctgacctgca acgagcagaa
     5401 tttcattact aaatcgggga tgcagcgtta cgcggctgaa cacaacatta ttgttgttgc
     5461 gccggacacc agtccgcgag gcagtcatgt cgcagatgcc gaccgttacg atctcgggca
     5521 aggtgccggg ttttacctga acgcaacgca agcgccgtgg aatgaacatt acaaaatgta
     5581 tgactatatc cgcaacgagc tgccggattt agtgatgcaa cattttccgg caacgaccag
     5641 aaagtctatc tctggtcatt ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa
     5701 cccagatgaa tatgtcagcg tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc
     5761 gtggggacag caagcctttg ccgcatatct tggtgaaaat aaagatgcct ggttggatta
     5821 cgatccggtg agtcttattt cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg
     5881 gttgagtgat gatttttacg cggaacagct gcggactcca aatcttgaaa agatctgcca
     5941 ggagatgaat atcaagacgt taatccgtta tcacgagggt tacgaccaca gctattattt
     6001 tgtctccagt tttattggcg agcatattgc ctaccacgcc aataaactga atatgcgttg
     6061 ataatagtgc acgactgccg gatgcggcgt gaacgcctta tccggcctac acttcgcccg
     6121 taaaccgtag gcctgataag acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg
     6181 gatgcggcgt gaacgcctta tccggtctac acttcgccca taaaccgtag gcctgataag
     6241 acgcgcatag cgtcgcatca ggcatctgcg cacgacttat ctatgtttac cacatcaaat
     6301 catcaggcac tttgaagtcg gcatacggat cgtcttcatc ctgctcttcg gcactgagcg
     6361 cgctgtgcaa cacaatactg ctggcatcac gctgagcaat tttatcggca acactggcgg
     6421 gaataatggt gtattcacct tcgctattgt tatcgaccaa caagcgggca atcgccagac
     6481 gaccattaat caactgcgcc tgtgtgagct tatcgacaaa aatcttctta atcagattgc
     6541 cgtcagtgaa gttaaaacca atatcgccat tggcaatggt gattcggttc atttcaatga
     6601 gctgcttcac ctgggcttta tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa
     6661 gctgtttatc acgctcaagc tgtgcctttt tattttcttc taccgcagcc cgagcttcac
     6721 gcgcctgaac gcgcgatttt ttcgccgttc tctcgacctt cgccgctttt ttgctggtga
     6781 ctaatcctgc tttgagcaac tgctcttgta aggtaagttt tgccatcttt gtttccaaac
     6841 ccgctgaata atttttagcg agtatacctg taaacgccgt gggtgtattg ggggcaagtc
     6901 cctttcgccc ctgcttaata aggcgcgtca ggcatcggca cacggctgca ttcttgatct
     6961 ccgactcatc aaaataaaca ccgccaaccc agcaacaaga atacccggtg cagaagcggc
     7021 cattacgccg accgtgccag tgcctaatgc cagcattttc ccggccagta acggaccgct
     7081 catagccccc agacgcccta ccgccacggc tgttcccaca cctgttgcgc ggatctgcga
     7141 cctgtaaaac aacggtgcca gggcatacaa aacgctttgc ccacctgtcg caaacaaccc
     7201 cgcgacaaat cccgccagca acataccgtt aaatgacgac accgttccaa gcgccagcag
     7261 cgaagctaac atgccgctat aaatcagtag cgacatggtt actggacgca gcttatccat
     7321 caatgcgccc aacattaacg tcccgcttgc cgcccccatt tgcagggcaa acatcacccc
     7381 tgccgcctgc gatggctgga atccttgctc caccaaaagt agcggtagcc agttgatcaa
     7441 catataaacc accagcagag tgaagaaata acacaaccac agcagcagcg tcgcggttgc
     7501 cgtttctggc gcaaataagg cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac
     7561 cgctgactcc ggcagccagc gcattaatag cggcaccaga atcaacggca ccacaccacc
     7621 tacccaaaac accgtttgcc atgctaagtt tgcccccgcg aaacccagtg tcgccgccag
     7681 cgccgcgcca atgggaacac cgcaatacat caggctcact gccgtcccac gaaaacgtgg
     7741 acccgcggct tcagacgtca gggcgataag attcggcaac gccgccccca gcccgacacc
     7801 ggtcatcagc cgcgcaaaga ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga
     7861 gaacaaacca aacagcgcaa ctgagccaat caaaatacgc ttacgaccat aacggtctgc
     7921 cagcatcccg ccaaccaacg cgccgggtag caaaccgagt attccggcgc taaatatcca
     7981 gcccatttgc attttatcga gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc
     8041 cgcctgaaga tccagccctt ccatcagagc gaccaaaaaa caaagcccga tagtcagcat
     8101 caggcgggat gaagatgatg aaggggtacg agtcgacata agatgaacct cgttgcgagt
     8161 gcaggtgaat cgcgactgat acattgccag ccgcgtaatc agaactattt aatgactgcc
     8221 tacgggcgaa atgtaggccg gacaagacgt ttaccgcatc cggcagtcgt gtgcagatgc
     8281 ctgatgcgac gctaacgcgt cttatcaggc ctacaaattc tcacgccacc cgtaggccgg
     8341 ataaggtgtt tacgccgcat ccggcagtcg tgctgggatg ggcatacaaa atgcacgttc
     8401 tcggcagtca tcattatttg ttgttgcgca gatccagcgc cacgtcaacg atcatatcct
     8461 cctggccgcc aaccatccgg cgtttgccca gctcaacgag aatatccacc gcacttaagc
     8521 catagcgcgc cgccgccgtt tcacagtgac gcaggaagct cgagtaaaca ccagcgtatc
     8581 ccagcgccag cgtttcgcga tcgactcgta ccggtcgatc ctgcaacgga cgcaccaggt
     8641 cgtcggcggc atccattaac gcatagagat cggtcccatg ctgccagccc agtttatccg
     8701 cggcggcaat aaacacttcc agcggtgcgt tacctgcgcc cgcgcccatt cccgcgaggc
     8761 tggcgtcgat tcggtcgcag ccctcttcca ccgccgcgat agagttcgcc acgccaagac
     8821 tcaggttatg gtgagcgtgc atgccagttt gcgtttctgg tttcagctct gctttcaggg
     8881 cgcgaaaacg gtcacggata tcgctcatgt tcatcgcacc gccagaatcc accacataaa
     8941 tacaggtcgc accgtagcct tccatcagct ttgcctgctt tgcgagattc tccggcgtgg
     9001 tcatatggct catcatcaga aaaccaacgg tgtccattcc gagctcgcgg gcatactgaa
     9061 tatgctgggc ggaaacatca gcttcggtac agtgcgttgc cacacgaacc acccgcgcgc
     9121 cagcctgcca ggcatttttc agatcgtgaa tagtgccgat tcctggcagc aacaacgtcg
     9181 cgattttggc gtgcttcacc acatccgccg ctgcttcaat ccattcaagg tcgctatgtg
     9241 cgccgaaacc atagttaaag ctggaaccct gcaaaccgtc gccatgggcc acttcaatcg
     9301 aatccacgcg ggcatcgtcc agtgctttgg caatctggcg aacgttttcc agcgaatact
     9361 gatgacgaat ggcgtgcata ccgtcacgca acgtgacgtc cgagatataa agttttttac
     9421 cgttcatgcc gcttctcctg ccttgcgcgc cagtgactgg gccatttttt ccgctgtcgc
     9481 cagcgcactg gaagtcataa tgtcgaggtt gcccgcatag gcaggcagat aatgcgctgc
     9541 gccttcgatt tccagccaga ccgctgtttt cagtccggag aattgcccca cgcccggtaa
     9601 attgaccggt ttatcctgcg ggataacttc aaactgcacg cgctgtttca ggcgataacc
     9661 cggtacgtaa gcctgcaccg cctcagccat ttcattgatt gaggcttcga tatcatcttg
     9721 tgaagcttcg tcgctcaata catacaccgt gtcacgcatc atcaacggtg gctctgctgg
     9781 gttaagcaca ataatcgcct tcccttttgc cgcgccgccc accacttcaa tggctcggga
     9841 agtggtttcc gtaaattcat cgatattggc acgcgtgcca ggtccggcag atttactggc
     9901 gatagaagcg ataatttcgg cgtaatgaac acgcgccacg cgtgaaactg ccgccaccat
     9961 tggaatggtg gcctggccgc cgcaggtgac catgttgacg ttcagttgat cgacgttcgc
    10021 ctcgaggtta accaccggca cgcagtaagg gccgatggca gcaggcgtca ggtcaattaa
    10081 gcgaatatcc ggtttcgctt cgcgtaaagc ggcatcgttt ttcacatgcg cgcccgcgct
    10141 ggtcgcatca aatacaatgt cgatatcagc aaattcaggc atgttcatca gcccgatcac
    10201 cccttcatgg gtggtggcga cgcccatacg tctggcacgc gccagaccgt cggactgagg
    10261 atcaatgccg accatcaccg ccatctccag atgctgaccg tgacgcaaaa ttttaatcat
    10321 cagatcggtg ccaatgttgc cagaaccgat aatggcgact ttacgcttac tcatgacaga
    10381 cttccttttg gggctgcgct tgaaaatgtc gcagcaactg aacctatgcc ttcaatatgg
    10441 gcttcaaaac gatcgcccgc attcaccgcc accatcgggc ctaatgcccc ggtaagaatg
    10501 atatctccgg cgcgcagcgg ttcacccaga ctggccattt tgcgtgccag ccagacggcg
    10561 gcattaagcg gatgtcccag gcattcgctg ccgcgcccgc tggaaacctc ttcgttatta
    10621 cgcgtcatct tcatggcgca gtttttcagg tctaaccccg ccggacgttg cgccggaccg
    10681 ccgatgacat acaccccgca ggaagcgtta tctgccacgg tatcgacaaa ctgaatcgac
    10741 cagtcgcgaa tgcggctccc caccacttcc agcgccggaa gtacccattc aatggcgtta
    10801 tacagttcgt cgaaggtgat atcagttgcg ggtaaatcgc ggttcaacac cagtgcgatc
    10861 tccgcttcaa tgcggggttg cagaacacgg gaaaaaggaa tgatttcgtt atcgccataa
    10921 cacatgtcgg caaataacgt cccaaaatcc ggttgatcaa cgcccagttg ttgttgcact
    10981 ttcggatgcg tcaggcccac tttacgccct accacgcgac gcccctgcac aacgtcatat
    11041 tgcacattta tgtgctgaat ggcgtaggcc gcttcagcgt tatcgatacc aatcagatcg
    11101 cgcagcggcg caatcgcttc gccctgctct gcggcgcggc gtaaatccgc cgccagttgc
    11161 tcaagagtat gcttcgtcat gaccattcct taagggcgtg cgaggaaatt cagcaccaat
    11221 tgattgaaag cgtcggcatg ttcccactgc gcccagtgac cacagtcgcg gaagatatgc
    11281 agttcagaac cggcaatgcc ggacagcaga cgcagacccg catccatcgg cacaaagcgg
    11341 tcgttgcgcc cccagacaat cagggtttgc gctttgattt ccgccagacg tgggccaaaa
    11401 tccgggaact gtttcggatt agcttccagg ctcttaacga agttttccag gtgatcgcgg
    11461 cgcgacagca tattattcag gcgcgcttca aacagggcgt cggtcaaatc gctggtatcg
    11521 aaaacgaaga tatccatcat cagcttcagg ttttcgatag tcggctgacg ataaagctga
    11581 ttcagtcgct taataccttc ggttggcatc ggcgtaaaca aactcatgcc gcccgtaccg
    11641 ccgcccatca gcaccagttt gccgacgcgc tccggccagt taagggtgaa cgccacagaa
    11701 ctatggccgc ccatcgagtt gcccagcagg tggattttgg cgatatccag ttgatccacc
    11761 acgcttttca ggattcgtgc attaagatcc gatcgtgaac cactattaac gatcgaatcg
    11821 cttttgcccc aacccggaca atccagcagg attacccgat agcccgcctc taccagcgga
    11881 tcgatattgc ggctgaagtt cgcccagcca gtagcacccg ggccggaacc atgcagcagg
    11941 acaacggttt cgtcgccttg tccgcagtca ttaaaatgga tgcgcagcgt tttacccgct
    12001 tcttctacat tcagaaaacg gctggtggcg gcttcggttt gtggctgata actcatcatc
    12061 ttctcctgca tattcagttc tctgttctgg cgcttaacga gccaaatccg gcaatccact
    12121 ccgggattgg gcggtaataa cgcccttcgc tacgccagtt gccaaatgta gaaatagcgg
    12181 caaaagcggc gacccaggtt ttgatttcat gtgtcgactt tccggcaatg gcggaaagct
    12241 cttcgttact gacggcatcc agttcctgta tgcgtccctg ctccagcaaa gtcatgaact
    12301 ggttatccca aatcgggttg agcggatgca gagttctctg atcctcaaca aacttctcag
    12361 cggcgctaat cacccgttgc tgacgcaatt cgcgctcact ggcgggtaaa tctttcccgc
    12421 tccccaacag acggtcgcgc atatgggcat cggctttcgc cagttcgggc accggcggct
    12481 gatgggaaag cccaccggaa cccaggaaca gcacgcgttt attgagagtg ctggtgaaac
    12541 gtccaatggc ttcacccaac atgcgggtac gctggaaacc gggcagcggc gtggcgacac
    12601 cgttgatgaa cacaggcaga actggcacct tatccagccc accgagcagg aactccagcg
    12661 gctgggcgaa cccgtggtcc acctgcatac agtaagaaac ggcaagatcg atcccgctct
    12721 tcatgacagc atgcgcacag gcctccgcca gctccacagg cacgggcagc tctcctgccg
    12781 cactgccgaa atcaccaatt gccgtcgctc caacgcctaa acagaacggt ggcatcacgt
    12841 catagaaaaa gccgttgtag tgatctggcg caaacagcac caccagttca ggggagaaag
    12901 ccgcaatacg ctcgcgggcg ctggcaatca cgccattgac ctcatcgagt acctcttgcg
    12961 ccgggtcgac ataccccacc aacggcgagt gggaaagaca gtgaagataa gcgtgcatat
    13021 caggctacct tttcgacaga aacgtcggca tcagggcggg tcagcgtcat caccgacgcc
    13081 agtttattca gcgtattgcc cagggtttgc ggaatggcgg tggcggcaac aaagcgatcc
    13141 gggcgcatca ccaccagcga agcattgtgt tgcgcgaacc agctacgcag acgaccttgc
    13201 gtatcgccca cgcgtagcac gccgtcgtgg ttatcctgtg cggtatgaat ttgcacttcc
    13261 ggcaccacct gaatgaagcg tgtgcccaac gcgcgccact gctggatttg ctcgtcgctc
    13321 atcccccaca gtggattgca tccccagcca attaccgcga agttcgcgcc aatcgcgtta
    13381 tcgagcagcg tcacgtcgcc gttttccagc gtgactttcg gctgaataaa catcttgccg
    13441 accggagagt gcttcgcttc gccctcacgt accagcgcac cgccgtaata ttgcggcatc
    13501 ggcttgaagc gcatttcgag gaagtagcgt tttactggcg gcagataatt cagtagccag
    13561 gaaacgccgt cacgtaacgt accctgccag cgtttcggcg gagccagcac gttgcccgcc
    13621 gtcacggaca ggtcaatcat cgctttggcg tgatcgcgac gttcttgttg ataggtatcg
    13681 agcagcgcat cgcgggcttt cccctggata accaacgcca gtttccatgc gaggttaaag
    13741 gcgtcgcgca taccactgtt atagccctgc ccctgccata ccggcatgat gtgcgcggca
    13801 tcgcccgcca gcagtacgcg atcaatacgg aaacgttgcg ccagtcgcgc gttgtgggtg
    13861 tagacacgct ggcgaatcaa ttcaacattg tccggattag gcagcacttt gcttaacagc
    13921 ttgcgcatat tttgcggctc acgcagctgt tcttcggttt ctcccggcat caccataaat
    13981 tcaaagcgac gtaccgcatg aggcagcgcg gcagaaacat acgggcgcac cggatcgcaa
    14041 cataaataga tatgcggcgt acttaacgga tcgttggcga tatccaccac aatccactga
    14101 tttggcgcag ttttaccttc aaacggcaca ttcagggtgc gacgaacaaa acttgcccca
    14161 ccatcacagg ccaccagcca ctgggctttg actatttccc gctgcccttc tgccgttttc
    14221 aggtgcaagg tcacttcgtc attttgctgg ctgaaggcct ccagctcgcg ggaaaacaag
    14281 cagcgcacat tcggaaaacg cgacaaccct tccagcatca ccgcatcgac ctgaggctga
    14341 ataaaggcgt tacggcgcgg ccagccaaat tcatcggtca ttggctgaat atcagcaaaa
    14401 cagcggcctt tcggggtgag aaaacgcatc gcgtgccacg gcgtagtgtg cggcagaaca
    14461 tcatcgacca ggccgaccga ctgcatggtg cgcagcgcct cgtcatcaat accaatcgca
    14521 cgcgggtagt cgatcaactt atcgagtttc tccaccacca gcacgtcaat gcccatctgg
    14581 ccgagatagt tcgccatcat cagcccgacc ggaccggcac cagcgatcgc cacctgaacg
    14641 ctatggttaa cagcaggctg gatgtcaggg tgttgtattg ccatttcagt acctcacgac
    14701 tcggacaaaa tgccgttgcg cgcacagtac ggcgcaactt attttgttaa aaacatgtaa
    14761 atgatttttt attgtgcgct cagtatagga agggtgtttt cggctacaat caaaacatgc
    14821 ccgaatgtgc accaggtgca caacgttgtt ttaactatag aaatgtcaat taatatgcag
    14881 aacaatgagc agacggaata caaaaccgtg cgcggcttaa cccgcggtct aatgttatta
    14941 aatatgttaa ataaacttga tggcggtgcc agcgtcgggc tgctggcgga actcagcggc
    15001 ctgcatcgca ccactgtgcg gcgactgctg gagacgctgc aggaagaggg atatgtccgc
    15061 cgtagcccct ccgatgacag ttttcgtctg accatcaaag tgcggcaatt aagcgaagga
    15121 tttcgtgacg aacagtggat ttctgcactg gcggccccgc tgctgggcga tctgttgcgc
    15181 gaagtggtat ggccaacaga tgtgtccacc ctggatgttg atgcaatggt ggtacgcgaa
    15241 accacccacc gtttcagccg cttatccttt caccgggcaa tggtcgggcg acgtttaccg
    15301 ctcctgaaaa ccgcctcggg cctgacctgg ctggcctttt gcccggaaca agaacgcaag
    15361 gaattaatcg aaatgttagc cgcccgcccc ggtgatgact atcaactggc acgagaaccg
    15421 ttaaagctgc aagccattct ggcgcgcgcg cgcaaagagg gttacggaca gaactaccgc
    15481 ggctgggatc aggaggagaa gatcgcctct atcgccgtac cgctgcgcag tgaacaacgg
    15541 gtgattggct gtctgaatct ggtgtatatg gcgagcgcaa tgaccattga acaggcagcg
    15601 gaaaagcatc ttccggcgct acaacgggta gcacaacaga tcgaagaagg ggttgaatcg
    15661 caggctattc tggtggccgg acggcgaagc ggcgtgcatt tacgttgaca ccatcgaatg
    15721 gcgcaaaacc tttcgtggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt
    15781 gaatgtgaaa ccagtaacgc tatacgatgt cgcagagtat gccggtgtct cttatcagac
    15841 cgtttctcgc gtggtgaacc aggccagcca cgtttctgcg aaaacgcgag aaaaagtgga
    15901 agcggcgatg gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa
    15961 acagtcgttg ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat
    16021 tgtcgcggcg attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt
    16081 agaacgaagc ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt
    16141 cagtgggctg atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc
    16201 ctgcactaat gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat
    16261 tattttctcc catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca
    16321 ccagcaaatc gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc
    16381 tggctggcat aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga
    16441 ctggagtgcc atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc
    16501 cactgcgatg ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga
    16561 gtccgggctg cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag
    16621 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
    16681 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
    16741 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
    16801 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
    16861 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
    16921 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
    16981 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
    17041 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
    17101 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
    17161 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc
    17221 gatcttcctg aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat
    17281 gcgcccatct acaccaacgt gacctatccc attacggtca atccgccgtt tgttcccacg
    17341 gagaatccga cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa
    17401 ggccagacgc gaattatttt tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggt
    17461 cgctgggtcg gttacggtca ggacagtcgt ttgccgtctg aatttgacct gagcgcattt
    17521 ttacgcgccg gagaaaaccg cctcgcggtg atggtgctgc gctggagtga cggcagttat
    17581 ctggaagatc aggatatgtg gcggatgagc ggcattttcc gtgacgtctc gttgctgcat
    17641 aaaccgacta cacaaatcag cgatttccat gttgccactc gctttaatga tgatttcagc
    17701 cgcgctgtac tggaggctga agttcagatg tgcggcgagt tgcgtgacta cctacgggta
    17761 acagtttctt tatggcaggg tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt
    17821 gaaattatcg atgagcgtgg tagttatgcc gatcgcgtca cactacgtct gaacgtcgaa
    17881 aacccgaaac tgtggagcgc cgaaatcccg aatctctatc gtgcggtggt tgaactgcac
    17941 accgccgacg gcacgctgat tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg
    18001 attgaaaatg gtctgctgct gctgaacggc aagccgttgc tgattcgagg cgttaaccgt
    18061 cacgagcatc atcctctgca tggtcaggtc atggatgagc agacgatggt gcaggatatc
    18121 ctgctgatga agcagaacaa ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg
    18181 ctgtggtaca cgctgtgcga ccgctacggc ctgtatgtgg tggatgaagc caatattgaa
    18241 acccacggca tggtgccaat gaatcgtctg accgatgatc cgcgctggct accggcgatg
    18301 agcgaacgcg taacgcgaat ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg
    18361 tcgctgggga atgaatcagg ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa
    18421 tctgtcgatc cttcccgccc ggtgcagtat gaaggcggcg gagccgacac cacggccacc
    18481 gatattattt gcccgatgta cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg
    18541 aaatggtcca tcaaaaaatg gctttcgcta cctggagaga cgcgcccgct gatcctttgc
    18601 gaatacgccc acgcgatggg taacagtctt ggcggtttcg ctaaatactg gcaggcgttt
    18661 cgtcagtatc cccgtttaca gggcggcttc gtctgggact gggtggatca gtcgctgatt
    18721 aaatatgatg aaaacggcaa cccgtggtcg gcttacggcg gtgattttgg cgatacgccg
    18781 aacgatcgcc agttctgtat gaacggtctg gtctttgccg accgcacgcc gcatccagcg
    18841 ctgacggaag caaaacacca gcagcagttt ttccagttcc gtttatccgg gcaaaccatc
    18901 gaagtgacca gcgaatacct gttccgtcat agcgataacg agctcctgca ctggatggtg
    18961 gcgctggatg gtaagccgct ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt
    19021 aaacagttga ttgaactgcc tgaactaccg cagccggaga gagccgggca actctggctc
    19081 acagtacgcg tagtgcaacc gaacgcgacc gcatggtcag aagccgggca catcagcgcc
    19141 tggcagcagt ggcgtctggc ggaaaacctc agtgtgacgc tcccctccgc gtcccatatc
    19201 atcccgcaac taaccaccag cgaaacggat ttttgcatcg agctgggtaa taagcgttgg
    19261 caatttaacc gtcagtcagg ccttctttca cagatgtgga ttggcgatga aaaacaactg
    19321 ctgacgccgc tgcgcgatca gttcacccgt gcaccgctgg ataacgacat tggcgtaagt
    19381 gaagcgaccc gcattgaccc taacgcctgg gtcgaacgct ggaaggcggc gggccattac
    19441 caggccgaag cagcgttgtt gcagtgctcg gcagatacac ttgccgacgc ggtgctgatt
    19501 acgaccgctc acgcgtggca gcatcaggga aaaaccttat ttatcagccg gaaaacctac
    19561 cggattgatg gtagtggtca aatggcgatt accgttgatg ttgaagtagc gagcgataca
    19621 ccgcatccgg cacggattgg cctgacctgc cagctggcgc aggtcgcaga gcgggtaaac
    19681 tggctcggat tagggccgca agaaaactat cccgaccgcc ttactgcggc ctgttttgac
    19741 cgctgggatc tgccattgtc agacatgtat accccgtacg tcttcccgag cgaaaacggt
    19801 ctgcgctgcg ggacgcgcga attgaattat ggcccacacc agtggcgcgg cgacttccag
    19861 ttcaacatca gccgctacag tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg
    19921 cacgcggaag aaggcacatg gctgaatatc gacggtttcc atatggggat tggtggcgac
    19981 gactcctgga gcccgtcagt gtcggcggaa ttccagctga gcgccggtcg ctaccattac
    20041 cagttggtct ggtgtcaaaa ataataataa ccgggcaggc catgtctgcc cgtatttcgc
    20101 gtaaggaaat ccattatgta ctatttaaaa aacacaaact tttggatgtt cggtttattc
    20161 tttttctttt acttttttat catgggagcc tacttcccgt ttttcccgat ttggctacat
    20221 gacatcaacc atatcagcaa aagtgatacg ggtattattt ttgccgctat ttctctgttc
    20281 tcgctattat tccaaccgct gtttggtctg ctttctgaca aactcgggct gcgcaaatac
    20341 ctgctgtgga ttattaccgg catgttagtg atgtttgcgc cgttctttat ttttatcttc
    20401 gggccactgt tacaatacaa cattttagta ggatcgattg ttggtggtat ttatctaggc
    20461 ttttgtttta acgccggtgc gccagcagta gaggcattta ttgagaaagt cagccgtcgc
    20521 agtaatttcg aatttggtcg cgcgcggatg tttggctgtg ttggctgggc gctgtgtgcc
    20581 tcgattgtcg gcatcatgtt caccatcaat aatcagtttg ttttctggct gggctctggc
    20641 tgtgcactca tcctcgccgt tttactcttt ttcgccaaaa cggatgcgcc ctcttctgcc
    20701 acggttgcca atgcggtagg tgccaaccat tcggcattta gccttaagct ggcactggaa
    20761 ctgttcagac agccaaaact gtggtttttg tcactgtatg ttattggcgt ttcctgcacc
    20821 tacgatgttt ttgaccaaca gtttgctaat ttctttactt cgttctttgc taccggtgaa
    20881 cagggtacgc gggtatttgg ctacgtaacg acaatgggcg aattacttaa cgcctcgatt
    20941 atgttctttg cgccactgat cattaatcgc atcggtggga aaaatgccct gctactggct
    21001 ggcactatta tgtctgtacg tattattggc tcatcgttcg ccacctcagc gctggaagtg
    21061 gttattctga aaacgctgca tatgtttgaa gtaccgttcc tgctggtggg ctgctttaaa
    21121 tatattacca gccagtttga agtgcgtttt tcagcgacga tttatctggt ctgtttctgc
    21181 ttctttaagc aactggcgat gatttttatg tctgtactgg cgggcaatat gtatgaaagc
    21241 atcggtttcc agggcgctta tctggtgctg ggtctggtgg cgctgggctt caccttaatt
    21301 tccgtgttca cgcttagcgg ccccggcccg ctttccctgc tgcgtcgtca ggtgaatgaa
    21361 gtcgcttaag caatcaatgt cggatgcggc gcgagcgcct tatccgacca acatatcata
    21421 acggagtgat cgcattgaac atgccaatga ccgaaagaat aagagcaggc aagctattta
    21481 ccgatatgtg cgaaggctta ccggaaaaaa gacttcgtgg gaaaacgtta atgtatgagt
    21541 ttaatcactc gcatccatca gaagttgaaa aaagagaaag cctgattaaa gaaatgtttg
    21601 ccacggtagg ggaaaacgcc tgggtagaac cgcctgtcta tttctcttac ggttccaaca
    21661 tccatatagg ccgcaatttt tatgcaaatt tcaatttaac cattgtcgat gactacacgg
    21721 taacaatcgg tgataacgta ctgattgcac ccaacgttac tctttccgtt acgggacacc
    21781 ctgtacacca tgaattgaga aaaaacggcg agatgtactc ttttccgata acgattggca
    21841 ataacgtctg gatcggaagt catgtggtta ttaatccagg cgtcaccatc ggggataatt
    21901 ctgttattgg cgcgggtagt atcgtcacaa aagacattcc accaaacgtc gtggcggct
//
LOCUS       DAZKIV010000073        21765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZKIV010000073 DAZKIV010000000
VERSION     DAZKIV010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.073"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1208
                     /locus_tag="ACHOT6_002681"
     CDS             <1..1208
                     /locus_tag="ACHOT6_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000098090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9947874.1"
                     /translation="LRMNESLGITPEDFANISSIFFVSYLIFQIPSSIGLQKLGARKW
                     ISAIIVGWGTVTTLIFFAKDTQHILLARVFLGIFEAGFFPGMVYYLACWFPARERGKV
                     NSFFMLSIAVASVLAAPMSGWIIEHMNTGNYEGWRWLFAIEGIPTVLLGALTFYLLPD
                     RPEKARWLTPSQVTALVDKLNSDNEKAAALNKNTNASFLSIIKNPVLLQLSFAYMFIQ
                     AAALAANYWLPGLVKGFSSDFTDTDVGLIMSIPFIFAMFSMPLWGWHSDKKNERKWHA
                     ALPMLVAGCGFLMIALVPSMTLRMMGLTLYGVGILSYYGPYWALPSALLSPSGLAISI
                     AFINSCSSLGGFLINKSLGYVSTHYGATGIFIVEAILCFIAVAILLSMKIDNKKDNAQ
                     KENIISHTK"
     gene            1221..2423
                     /locus_tag="ACHOT6_002682"
     CDS             1221..2423
                     /locus_tag="ACHOT6_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HFP9947875.1"
                     /translation="MKITSVEIFDCELKKRDQTMASYNPVLIRVNTDTGISGVGEVGL
                     AYGAGAKSGVGIIRDLAPLIIGEDPLNIEKIWEFLFRKTFWGMGGGNVFYAGMSAIDI
                     ALWDIKGKYLNVPVYQLLGGKTNDKLRTYASQLQFGWGEKRQILVTPEEYAEAARAAI
                     AEGYNAIKVDPLEIDLHGGDCVFQNKNRNYSGLLLAEQLKMGEARIAAMREAMGDDAD
                     IIVEIHSLLGTNSAIQFAKAIEKYRIFLYEEPIHPLNSDNMQKVARSTSIPIATGERS
                     YTRWGYRDLLEKQSIAVAQPDLCLCGGITEGKKICDYANIYDTTVQVHVCGGPVSTVA
                     ALHMETAIPNFIIHEHHTNAMKACIRELCTYDYQPENGYYVAPELPGLGQELNDEIVQ
                     QYLAYVIK"
     gene            2649..5276
                     /gene="gyrA"
                     /locus_tag="ACHOT6_002683"
     CDS             2649..5276
                     /gene="gyrA"
                     /locus_tag="ACHOT6_002683"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFP9947876.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            5425..7113
                     /gene="yfaA"
                     /locus_tag="ACHOT6_002684"
     CDS             5425..7113
                     /gene="yfaA"
                     /locus_tag="ACHOT6_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFP9947877.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETIPVYQLRYNGNNALMFATYQDKMLVFSCTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            7110..7733
                     /gene="yfaT"
                     /locus_tag="ACHOT6_002685"
     CDS             7110..7733
                     /gene="yfaT"
                     /locus_tag="ACHOT6_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFP9947878.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            7757..12271
                     /locus_tag="ACHOT6_002686"
     CDS             7757..12271
                     /locus_tag="ACHOT6_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFP9947879.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSWQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVNVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSYAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDDFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDISDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEYWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKMAQQSNIPVTVERQLYRLIPGEEEMSFILQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            12272..13921
                     /gene="yfaQ"
                     /locus_tag="ACHOT6_002687"
     CDS             12272..13921
                     /gene="yfaQ"
                     /locus_tag="ACHOT6_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFP9947880.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            13926..14702
                     /gene="yfaP"
                     /locus_tag="ACHOT6_002688"
     CDS             13926..14702
                     /gene="yfaP"
                     /locus_tag="ACHOT6_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFP9947881.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            14977..17826
                     /gene="rcsC"
                     /locus_tag="ACHOT6_002689"
     CDS             14977..17826
                     /gene="rcsC"
                     /locus_tag="ACHOT6_002689"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFP9947882.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(18026..18676)
                     /gene="rcsB"
                     /locus_tag="ACHOT6_002690"
     CDS             complement(18026..18676)
                     /gene="rcsB"
                     /locus_tag="ACHOT6_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFP9947883.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(18693..21365)
                     /gene="rcsD"
                     /locus_tag="ACHOT6_002691"
     CDS             complement(18693..21365)
                     /gene="rcsD"
                     /locus_tag="ACHOT6_002691"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFP9947884.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTENVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5382 a         5168 c         5814 g         5401 t
ORIGIN      
        1 ctttacgcat gaatgaatcc cttggtatta caccagagga ttttgctaat atatcttcaa
       61 tattttttgt gtcctattta atttttcaaa ttccgagcag tattggattg caaaagttag
      121 gtgcaagaaa atggattagc gccattattg ttggttgggg aacagtaaca acgttaattt
      181 tctttgccaa agacacccag catatattgc tcgctcgggt ctttttggga atatttgaag
      241 caggtttttt ccctggcatg gtttactacc tggcatgttg gttcccggcg cgtgaacgtg
      301 gaaaagtgaa tagctttttt atgttatcaa tcgcggttgc atctgtgctg gcggcaccaa
      361 tgtctggttg gattattgaa catatgaata ccggtaacta tgaaggatgg cgctggcttt
      421 ttgctattga aggcattcct acggtgttat tgggggcgct gaccttttat ttactgccag
      481 atcgtccgga aaaagcccgc tggcttacac catcgcaagt caccgcattg gtcgataaac
      541 ttaattctga taacgaaaaa gccgcagcgt taaataaaaa tacaaacgca tcttttctgt
      601 caatcattaa aaatccagta ttactccaac tctcatttgc ttatatgttt attcaggctg
      661 cggcgttggc ggcaaattac tggttaccgg ggttggttaa aggattttca tcggatttca
      721 cagataccga tgtcggtttg attatgagta ttccgtttat tttcgcgatg ttcagtatgc
      781 cattatgggg atggcattcc gataaaaaga atgaaaggaa atggcatgcc gcactaccga
      841 tgttggttgc aggttgcggt tttttaatga tcgcacttgt gccctcgatg acgctgcgca
      901 tgatgggttt aacattatac ggtgtaggta ttctcagtta ttacggtccg tactgggctt
      961 taccctcagc tttactttca ccatcaggcc tggcaatcag tattgcgttt attaactctt
     1021 gttcaagttt gggtggtttt ctgatcaata aatcacttgg ctatgtttct acacattatg
     1081 gtgccacggg gatatttatt gttgaggcga ttctttgctt tatcgccgta gctattttgt
     1141 tatcaatgaa aattgataac aaaaaagata acgcccagaa agaaaatatt atttcacata
     1201 ctaaataaat gaggctattt atgaagatta catctgtaga aatttttgac tgcgaactca
     1261 aaaaaagaga tcaaactatg gcctcttata atccagtact tattcgggtt aataccgata
     1321 ctggaataag tggtgttggc gaagtaggat tagcctatgg tgcaggtgca aaatctggtg
     1381 tgggtattat cagagattta gcccctctca tcattggtga agatccgttg aacattgaga
     1441 agatttggga attcctcttc aggaaaacat tttggggaat gggcggtggt aatgtttttt
     1501 acgcaggtat gagcgctatt gatattgcct tatgggatat taaaggtaaa tatttaaacg
     1561 tgccggttta tcaactactg gggggaaaga ctaacgataa attgagaact tacgccagtc
     1621 agttacaatt tggttggggc gaaaaaaggc aaatattagt aacgcctgaa gagtatgcag
     1681 aggcagcgcg tgccgctatt gcagaaggtt ataatgcgat aaaagttgat ccgcttgaaa
     1741 tcgatctgca tggcggcgat tgtgtctttc agaacaaaaa tagaaattac tctggcttat
     1801 tactggccga acaattaaaa atgggcgagg caagaattgc cgctatgcgt gaagcaatgg
     1861 gcgacgatgc tgatattatt gtagagattc attctcttct aggaacaaat tcagcgatcc
     1921 agtttgccaa agcaatagag aaatatcgta tttttctcta tgaagagcca attcatccat
     1981 taaattcgga taatatgcag aaagtcgccc gttcaacatc gattccgata gcaacaggtg
     2041 agcgttctta tactcgctgg ggatatcgtg atttactgga aaaacaatca attgcggtag
     2101 ctcaacctga tttgtgtctt tgtggtggca ttacagaggg aaagaaaatt tgtgattatg
     2161 ccaatatata tgacacaacc gttcaggtac atgtttgtgg cgggcctgtt tcaacagtag
     2221 cggcgctgca tatggaaact gcaataccta actttattat tcatgaacat cataccaatg
     2281 cgatgaaagc atgtattcgg gaactttgta cttacgatta tcaacccgag aatggctatt
     2341 atgtcgcgcc agagttgcct gggcttggtc aggaattaaa cgatgaaatc gtacaacaat
     2401 atcttgccta tgtgatcaaa taacagcgat ttaaggccat tatttctggt gtttattgag
     2461 gcacaggaag cgtgttgcgg tctttgttcc tttgccgttc accgccagcg cgatgtgtgc
     2521 tggcggtggg gtaaaacgca gtagatctac agcgcccgga agcctgcatt acgtttaccc
     2581 gtccgcaagc gattgtgtta taatctccga cctttgaatc cgggatacag tagagggata
     2641 gcggttagat gagcgacctt gcgagagaaa ttacaccggt caacattgag gaagagctga
     2701 agagctccta tctggattat gcgatgtcgg tcattgttgg ccgtgcactg ccagatgtcc
     2761 gagatggcct gaagccggta caccgtcgcg tactttacgc catgaacgta ctaggcaatg
     2821 actggaacaa agcctataaa aaatctgccc gtgtcgttgg tgacgtaatc ggtaaatacc
     2881 atccccatgg tgactcggcg gtttatgaca cgatcgtccg tatggcgcag ccattctcgc
     2941 tgcgttacat gctggtagac ggccagggta acttcggttc catcgacggc gactctgcgg
     3001 cggcaatgcg ttatacggaa atccgtctgg cgaaaattgc ccatgaactg atggctgatc
     3061 tcgaaaaaga gacggtcgat ttcgttgata actatgacgg cacggaaaaa attcctgacg
     3121 tcatgccaac caaaattcct aacctgctgg tgaacggttc ttccggtatc gccgtaggta
     3181 tggcaaccaa catcccgccg cacaacctga cggaagtcat caacggttgt ctggcgtata
     3241 tcgatgatga agacatcagc attgaagggc tgatggaaca catcccgggg ccggacttcc
     3301 cgacggcggc aatcattaac ggtcgtcgcg gtattgaaga agcttaccgt accggtcgcg
     3361 gcaaggtgta tatccgcgct cgcgcagaag tggaagttga cgccaaaacc ggtcgtgaaa
     3421 ccattatcgt ccacgaaatt ccgtatcagg taaacaaagc gcgcctgatc gagaagattg
     3481 cggaactggt aaaagaaaaa cgcgtggaag gcatcagcgc gctgcgtgac gagtctgaca
     3541 aagacggtat gcgcatcgtg attgaagtga aacgcgatgc ggtcggtgaa gttgtgctca
     3601 acaacctcta ctcccagacc cagttgcagg tttctttcgg tatcaacatg gtggcattgc
     3661 accatggtca gccgaagatc atgaacctga aagacatcat cgcggcgttt gttcgtcacc
     3721 gccgtgaagt ggtgacccgt cgtactattt tcgaactgcg taaagctcgc gatcgtgctc
     3781 atatccttga agcattagcc gtggcgctgg cgaacatcga cccgatcatc gaactgatcc
     3841 gtcatgcgcc gacgcctgca gaagcgaaaa ctgcgctggt tgctaatccg tggcagctgg
     3901 gcaacgttgc cgcgatgctc gaacgtgctg gcgacgatgc tgcgcgtccg gaatggctgg
     3961 agccagagtt cggcgtgcgt gatggtctgt actacctgac cgaacagcaa gctcaggcga
     4021 ttctggatct gcgtttgcag aaactgaccg gccttgagca cgaaaaactg ctcgacgaat
     4081 acaaagagct gctggatcag atcgcggaac tgttgcgtat tcttggtagc gccgatcgtt
     4141 tgatggaagt gatccgtgaa gagctggagc tggttcgtga acagttcggt gacaaacgtc
     4201 gtactgaaat caccgccaac agcgcagaca tcaacctgga agatctgatc actcaggaag
     4261 atgtggtagt gacgctctct caccagggct acgttaagta tcagccgctt tctgaatacg
     4321 aagcgcagcg tcgtggcggg aaaggtaaat ctgccgcacg tattaaagaa gaagacttta
     4381 tcgaccgact gctggtggcg aacactcacg accatattct gtgcttctcc agccgtggtc
     4441 gcgtctattc gatgaaagtt tatcagttgc cggaagccac tcgtggcgcg cgcggtcgtc
     4501 cgatcgtcaa cctgctgccg ctggagcagg acgaacgtat cactgcgatc ctgccagtga
     4561 ccgagtttga agaaggcgtg aaagtcttca tggcgaccgc taacggtacc gtgaagaaaa
     4621 ctgtcctcac cgagttcaac cgtctgcgta ccgccggtaa agtggcgatc aaactggttg
     4681 acggcgatga gctgatcggc gttgacctga ccagcggcga agacgaagta atgctgttct
     4741 ccgctgaagg taaagtggtg cgctttaaag agtcttctgt ccgtgcgatg ggctgcaaca
     4801 ccaccggtgt tcgcggtatt cgcttaggtg aaggcgataa agtcgtctct ctgatcgtgc
     4861 ctcgtggcga tggcgcaatc ctcaccgcaa cgcaaaacgg ttacggtaaa cgtaccgcag
     4921 tggcggaata cccaaccaag tcgcgtgcga cgaaaggggt tatctccatt aaggttactg
     4981 aacgtaatgg tttagtggtt ggcgcggtac aggtagatga ctgcgaccag atcatgatga
     5041 tcaccgatgc cggtacgctg gtacgtactc gcgtttcgga aatcagcatc gtaggccgta
     5101 acacccaggg cgtgatcctc atccgtactg cggaagatga aaacgtagtg ggtctgcaac
     5161 gtgttgctga accggttgac gaggaagatc tggataccat cgatggcagt gccgcggaag
     5221 gggacgatga aatcgctccg gaagtggacg ttgacgacga gccagaagaa gaataatttt
     5281 acttcttcat gccaaaaggg agctatctcc cttgtttgaa ttgaaaagtc caggctgcaa
     5341 agtctgggct tttgtcgtat tagggcacgg taaagtttgg ctgtgcccgt aaaaaatggc
     5401 tggctataca caaggaatgt ggcaatgagt ggtgaaaaaa aggcgaaagg ctggcggttc
     5461 tatggtcttg taggttttgg cgcaatagca ctgctttccg ctggcgtctg ggcgttgcaa
     5521 tatgctggca gtgggccaga aaaaacgttg tcgccgctgg tggtgcacaa caatctgcaa
     5581 atcgatctca atgagccgga cctctttctc gacagcgact ctctgagcca gcttcccaaa
     5641 gatctcctca ccattccgtt tctccacgat gttctgagcg aagatttcgt tttctattat
     5701 cagaatcatg ccgatcgtct gggcattgaa ggcagcattc gtcgtattgt ctatgaacac
     5761 gatctcacgc tgaaagataa actcttttcg tcactcttag atcagcccgc gcaggcggcg
     5821 ctatggcacg ataaacaagg ccatctttca cattacatgg tgctgatcca gcgcagtggt
     5881 ttaagcaaac tgctggagcc attgttgttt gccgctacca gcgacagcca gttaagcaaa
     5941 acggaaatca gtagcatcaa gataaatagt gaaactattc ctgtttatca gttgcgctat
     6001 aacggcaata acgccctgat gttcgcgact tatcaggaca agatgctggt gttttcctgc
     6061 acggatatgt tgtttaaaga tgatcagcag gataccgaag ccacggcgat cgcaggtgat
     6121 ttgttgagcg gcaaaaaacg ctggcaagca agctttggcc tggaagagcg tgctgctgaa
     6181 aaaacgccag tacgccagcg catcgtggtc agcgccaggt tgctgggttt tggctaccag
     6241 cggttaatgc cttcttttgc tggcgtacgc ttcgaaatgg gtaacgacgg ctggcacagt
     6301 tttgtggcgt taaatgatga atccgccagc gtagatacca gtttcgattt tacgccggtc
     6361 tggaacagta tgcctgccgg ggccagcttc tgtgtggtgg tgccgtattc acacggtatt
     6421 gccgaagaga tgctttcgca catcagtcag gaaaacgaca agttgaatgg ggcgttagac
     6481 ggtgccgcgg ggctgtgctg gtatgcagac tcaaaattgc aaaccccgct gtttgtcggt
     6541 cagtttgatg gcactgccga acaggcgcaa ttgccaggga aactgtttac gcaaaatatt
     6601 ggtgcgcacg aaagcaaagc gccagaaggt gttttgccga taagccagac tcagcagggc
     6661 gaagcgcaaa tctggcgtcg cgaagtgagt tcccgatacg gtcagtatcc gaaagcgcag
     6721 gcggcgcaac ccgatcaatt aatgtcggat tattttttcc gcgtgtcgct ggcgatgcaa
     6781 aacaaaacgc tgcttttctc cctcgatgac acgctggtta ataacgccct gcaaacactg
     6841 aataaaaacc gcccggcaat ggtggatgta atacccactg atggcatcgt tccgctctat
     6901 atcaatccac aaggcgtggc gaaactgctg cgtaacgaaa cgttgaccag tctgccgaag
     6961 aatctcgaac cggtttttta taacgccgca caaactttat taatgccgaa gctggacgct
     7021 ttatctcaac aaccgcgtta tgtcatgaag ctggcccaga tggaacccgg tgccgcctgg
     7081 cagtggctac ccataacctg gcagccgcta tgaggcacgg gctgctggcg ctgatttgct
     7141 ggctgtgttg tgttgttgcc catagcgaaa tgctgaatgt cgaacaatcc gggctgtttc
     7201 gcgcctggtt tgtacgcatt gcacaagaac agctccgcca ggggccaagt ccacgctggt
     7261 atcagcagga ttgtgcgggc ctggtgcgat ttgcggcgaa cgagacgctg aaagttcacg
     7321 atagtaaatg gctaaaaagt aacggtttct ctagccagta tttgccgcca gagatgacgc
     7381 taacacctgg acagcgtcaa ctggcgcaaa actggaatca ggggaacggg aaaaccggcc
     7441 cctacgtgac cgcgattaat ttgattcagt acaacagcca gtttattggc caggacataa
     7501 accaggcgct gcctggcgat atgatttttt tcgatcaggg cgatgcccag cacttaatgg
     7561 tctggatggg gcgttacgtc atctaccaca ccggaagcgc cacgaaaact gacaacggaa
     7621 tgcgcgcagt cagtctgcaa caacttatga catggaagga cacccgatgg atacccaacg
     7681 attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga tagcagcgtg
     7741 cttgtccctg tctcttgtgg gaacagggct tgctaatgct gatgattcgc ttccttccag
     7801 taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca gttttagcag
     7861 cagtgaagag gcgaaagtgc gactggaagc gccggggcgt gattatcggc gctatcagat
     7921 ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga tggcattttt
     7981 gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg gcgacgggct
     8041 gaacaatacg ctgacctggc tgtgggataa ctggtacggc aaatctcgcc gcgtaatgca
     8101 gcgtactttc tcttctcagt catggcagaa tgtgactcag gcattacccg aattacatct
     8161 cggcaatgcc attattaaac cttcccgtta tgtacagaac aaccagtttt ccccgctgaa
     8221 aaaatatccc ttggtggaac agttccgtta tccactatgg caggctaaac cggtcgagcc
     8281 gcagcaaggg gtaaaactgg aaggcgcatc cagcaatttc atctcgccgc agccgggtaa
     8341 catttatatt cctctcggcc aacaagagcc gggactgtac ctcgtcgagg cgatggttgg
     8401 tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc gtggcgctta gcaaagtgtc
     8461 aggcaacgag cttctggtgt ggaccgcggg taaaaaacag ggtgaagcga agcccggctc
     8521 agagatcttg tggactgacg gtcttggcgt gatgacccgc ggtgtgaccg atgacagcgg
     8581 taccttgcag ttacaacata tatcgccaga acgctcatac attctgggta aggatgctga
     8641 aggcggcgtt tttgtctccg agaacttctt ttacgaaagt gaaatctaca acacccgctt
     8701 gtatatcttt accgatcgcc cgctatatcg cgcaggcgat cgtgtcgatg ttaaagtgat
     8761 gggccgcgag ttccacgatc cgttgcattc atcccccatc gtcagcgccc cggcgaagct
     8821 ttcggtgctg gacgccaacg gcagtctgtt gcaaaccgtc aatgtcacgc tggatgcgcg
     8881 caatggcggg cagggaagtt tccgcctgcc agaaaatgcc gttgccggag gttatgagtt
     8941 acgtcttgct taccgcaatc aggtctatag cagcagtttt cgcgtggcaa actacatcaa
     9001 gccacatttc gagattggtt tagctctcga caaaaaagag ttcaaaactg gcgaagcggt
     9061 cagcggcaaa ctgcaactgc tctacccgga tggcgagccg gtaaaaaatg cccgcgtgca
     9121 gttaagtttg cgcgctcagc aattatcaat ggtcggtaac gatttgcgtt atgccggacg
     9181 tttccccgtg tcgctggaag gcagcgaaac ggtgtccgac gccagcggtc atgtggcgtt
     9241 aaatctcccc gccgccgata aaccgagccg ttatttgtta accgtctccg ccagtgacgg
     9301 cgcggcgtat cgtgtcacca ccaccaaaga gatcctcatt gaacgcggtc tggcgcatta
     9361 ctcattaagt actgccgcac aatacagtaa tagcggcgag tcggttgtgt tccgttatgc
     9421 cgcgctggaa tcttcaaaac aggttcctgt tacgtatgaa tggttgcgtc tcgaagaccg
     9481 cacgagccat agcggagagc taccgtcagg cggcaaatcc tttaccgtca atttcgctaa
     9541 acctggcaac tacaatctga cattacgcga taaagacggc ttaattctcg ctgggttaag
     9601 ttatgccgtc agcggtaagg gcagcacggc gcatacgggt acggtagata tcgtggcgga
     9661 taaaacgctg taccagccag gcgaaaccgc gaagatgctg attacttttc cggagccaat
     9721 tgatgaagca ttattgacgc tggaacgcga tcgcgtggaa cagcagtcgc tgctttcgca
     9781 tccggcaaac tggctaacgc tacaacgttt aaacgatacc cagtatgaag cccgggttcc
     9841 agtgagcaat tcctttgcgc ctaacatcac tttttcggtg ctgtataccc gtaatggtca
     9901 gtacagtttt cagaacgccg ggatcaaagt tgccgttcct cagcttgata tccgggtgaa
     9961 aacggacaaa acccattacc agcctggtga acttgtcaat gtcgagttaa cctcgtcgct
    10021 gaaaggtaaa cctgtttctg cgcagctaac ggttggcgtg gtcgatgaaa tgatctacgc
    10081 gctgcaaccg gaaatcgcgc cgaatatcgg caaatttttc tatccgctgg ggcgtaacaa
    10141 tgtacgcacc agctccagtc tgtcgtttat cagctacgac caggcactct ccagcgagcc
    10201 ggttgcgcct ggcgcaacta accgcagcga gcggcgagta aaaatgcttg aacgtccacg
    10261 gcgtgaagag gtggataccg cggcatggat gccgtcactc acaaccgata aacaaggcaa
    10321 agcgtatttc acgttcctga tgcctgattc gttaacccgc tggcgtatca ccgcgcgtgg
    10381 gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat ctgcgttcgg aaaaaaatct
    10441 ctacatgaag tggagtatgc caacggtgta tcgcgtgggc gacaaaccgg cggcaggact
    10501 gtttatcttc agtcagcagg ataacgaacc ggtggcgctg gtgactaaat ttgcaggcgc
    10561 tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc tgacgcagaa
    10621 tattcagcaa tctggcttgt taagtgcaga actgcaacaa aatgggcaag tgcaggacag
    10681 cattagcaca aaactgtctt ttgtggacaa cagctggccc gttgaacagc agaaaaatgt
    10741 catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggcgagta atatccggct
    10801 acaaagtagt gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt tagtcgatga
    10861 accgtggggt ggggtgatca acaccggtag ccgtctgatc ccactcagtc tcgcctggcg
    10921 ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga ttcaggataa
    10981 ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt ggggtgaaga
    11041 tggcaatggt gacgacttcc ttacggcatg ggcatggtac gccgactggc aggccagcca
    11101 ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca gttatgccga
    11161 gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg cacaggagat
    11221 gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc ggcgcggaac
    11281 taaagatgaa gatttctctg aggaagacat cagcgatatc aatgacagcc tgatcctcga
    11341 tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga cgttgctgaa
    11401 aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt gggataaagc
    11461 ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg gtggtgacgc
    11521 tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta ttgagcgcgc
    11581 gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg ttttgcctgc
    11641 gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaatact ggcgttgggt
    11701 tggtcagggc gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc aaaatgtgca
    11761 ggtccgctgg cgtgagccgg caaaaatggc tcaacaaagt aacattccgg tgaccgttga
    11821 acgccagttg tatcggctta tccctggtga agaagagatg agctttattc tgcaaccggt
    11881 gaccagcaat gagattgaca gcgatgcgct gtatctcgat gaaatcacgc ttaccagcga
    11941 gcaggatgca gttctgcgct acggtcaggt ggaagtaccg ctgccaccgg gagccgacgt
    12001 tgagcgcaca acatggggca tttcggtcaa taaacccaac gccgcgaaac agcaggggca
    12061 attgctggaa aaagcgcgta atgaaatggg cgaactggcc tatatggtgc cggtgaaaga
    12121 actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag ggcaattcgt
    12181 tctgcctcct gctcgttatg tgcgttccta tgcacctgca cagcaaagtg ttgcggcagg
    12241 gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg tctggctatt
    12301 ggcgttggtt actcagccaa cgcttgcgga ggagacgccg ttacaactgg cactgcgtgg
    12361 tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccaaag tctcagcatt
    12421 acctgactcg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt acgcctggct
    12481 ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc cggaagaggt
    12541 ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac gttcctgtgg
    12601 gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc aatactggca
    12661 acagcgtcag gcgcctgcgt ggttagcgtc tcttactacg ctgaagccag aaacctcggt
    12721 aacggttaaa tcgctgctcg attcattagc gacgttacct gcgcaaaaca aagctcagga
    12781 agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca tgctcggtag
    12841 tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa tccgtcaggg
    12901 tggttttgcc ggctggttaa ccgatggtac accgctgtgg gttactggca gtggcaccag
    12961 caaaacggta ctaacccgtt atgcgacggt gcttaatcgg gttttgccag tgccgacgca
    13021 ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc tgaagaaaat
    13081 cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc gctatcgtgt
    13141 gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga ccttactgag
    13201 cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg ttgccagagt
    13261 tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg aaagccatga ccgtggcgat
    13321 tcgcactttt ttacagcaaa acgccaatcg ggaaggcgat tgcctgacca tccccgacag
    13381 cagcgcaacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa cgatgaccgc
    13441 ctggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca gtcgagctac
    13501 cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcagggag agcgttacga
    13561 ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag ctccacgctc
    13621 aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc cgcagtggcg
    13681 gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg tctgtcgttt
    13741 agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc gtaacttctt
    13801 tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct ttgatggtta
    13861 tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat taatggacta
    13921 atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct gtcgctcaca
    13981 gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat ccggcagagg
    14041 gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc aacatggcag
    14101 atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct gcggcgggta
    14161 aagttcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga attgaatccg
    14221 atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg caggtcatca
    14281 gcccggatgg gcaaagccgg caaaaaatgc agttttactc aacgccgggt acaggaacga
    14341 ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat ctcgatctcc
    14401 atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg aaaaacagtg
    14461 gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc atgccagcac
    14521 cagttcacgg gcgctatcag gtgtatatca actactatgg tgggcgcagc gaaacggaat
    14581 tgacgaccgc ccaactgacg ctgatcaccg atgaaggatc ggtcaatgag aaacaggaaa
    14641 catttattgt accgatgcgt aatgctggcg agctgacgct ggtgaaaagt tttgactggt
    14701 gatccatccc ttcacacaac ctggctctct cttttttcag ataagactgc tgggcgatct
    14761 gccgacgcca ggatataacc attaacaagc gtcggcagat tggtcatttc ttatttttcg
    14821 tactgagaac tgtccatcta ctccggcttt cctggttaag gcaatggttt ctaagcgata
    14881 cgcattattg caatggtgac agaacattgc ggtaacacgc ttttaccgct accttaacca
    14941 cactccatcg gtcacctgag gcggagcttc gcccctttga aataccttgc ttcttttcgt
    15001 acaaccctga aagcctcgcg ctacatgttc agagcattgg cgttagtgct ctggctgttg
    15061 attgcttttt catccgtttt ttacatcgtt aatgcgttac atcagcgaga atcggaaatt
    15121 cgtcaggaat ttaatctgag ttccgatcag gctcagcgct ttattcaacg cacctctgat
    15181 gtgatgaaag agctgaagta catcgccgaa aatcgcttat cggcagaaaa cggtgtgctt
    15241 tccccgcgtg gacgagaaac gcaggcggat gtgcctgcgt ttgaaccgct gtttgccgac
    15301 tccgattgtt ccgcaatgag taacacctgg cgaggttctc tggagtcatt ggcgtggttt
    15361 atgcgctact ggcgcgataa tttttctgcg gcttacgatc tcaaccgggt atttttaatc
    15421 ggcagtgata acctctgcat ggccaatttc ggtctgcgtg atatgccagt ggaacgcgat
    15481 accgcgttga aagctttgca tgaacgcatc aataaatatc gaaatgcacc acaagatgat
    15541 agcggcagta acctctactg gatcagcgaa ggtccgcgcc ctggcgtcgg gtatttttac
    15601 gcgttgacgc cagtttatct ggcgaaccgg ttgcaggcgc ttttgggtgt cgagcagacc
    15661 atccggatgg agaacttttt cttaccgggt acgttgccga tgggggttac cattcttgat
    15721 gaaaatggtc ataccctgat ttcgcttacc ggaccagaaa gtaaaattaa gggcgatcct
    15781 cgctggatgc aggaacgctc ctggtttggc tatacggaag ggttccggga gctggtgctg
    15841 aagaaaaatc tgccaccctc atcgctaagc atcgtgtatt cggtgccggt tgataaggtg
    15901 ctggaacgca ttcgcatgtt gatccttaac gcaattttgc tgaatgtgct tgccggagct
    15961 gcattgttta ctctcgcacg gatgtacgag cgacgtattt tcattccggc ggaaagcgac
    16021 gccctgcgac tggaagaaca tgagcagttc aatcgcaaga ttgtcgcctc cgcgccagtg
    16081 ggtatctgca ttttgcgtac cgctgatggc gtcaatattt taagtaacga actggcgcat
    16141 acctatctca atatgcttac gcatgaggac cgccaacgac tgacgcaaat tatctgtggg
    16201 cagcaggtca attttgttga tgtcctgacc agcaacaata ccaatctgca aatcagcttc
    16261 gtccattcgc gctatcgtaa tgaaaacgtg gccatttgtg tgctggtgga tgtttcttcg
    16321 cgcgtgaaga tggaagagtc gttgcaggag atggcacaag cagcggaaca ggcgagccag
    16381 tcaaaatcga tgttccttgc caccgtcagt catgagctgc gaacgccgct gtatggcatt
    16441 attggtaacc tggatctgtt gcaaaccaaa gagttaccga aaggcgtcga tcggctggtg
    16501 acggcaatga acaactcttc cagcctgttg ttgaaaatta tcagcgatat tctcgatttc
    16561 tcgaagattg aatcggaaca gttgaagatc gaaccgcgtg agttttcacc gcgtgaagtg
    16621 atgaaccaca tcaccgccaa ctatttaccg ctggtggtac gcaagcagtt aggcttgtac
    16681 tgctttattg aaccggatgt gccagtggcc ttaaatggcg acccgatgcg tttacagcag
    16741 gtcatctcca acctgttgag taacgccata aaattcaccg ataccggctg tatagttttg
    16801 catgtccgcg ctgatggcga ttatctctct atccgtgttc gcgataccgg cgtggggatt
    16861 ccggcgaaag aagtggtgcg cttgtttgat cccttcttcc aggtcggaac gggcgtacag
    16921 cgaaacttcc aggggaccgg tctgggtctg gcgatttgtg aaaaactgat cagcatgatg
    16981 gacggcgata tctcggtaga ttcagaaccg ggaatgggca gccagtttac cgtgcgtatt
    17041 ccgttgtacg gcgctcagta cccgcagaaa aaaggcgtgg aagggttgag tggtaaacgc
    17101 tgctggctgg cggtccgcaa tgcgtcgctc tgtcagttcc tggaaaccag tttgcagcgc
    17161 agcggcatcg tcgttacaac atacgaaggg caggaaccga ctcccgaaga tgtgttaatt
    17221 actgacgagg tagtgagtaa aaaatggcag ggtagagcgg tagtgacctt ctgtcgtcgc
    17281 catattggta ttccgctgga gaaagcgcca ggggagtggg tacacagtgt ggctgccccg
    17341 catgagctac cggcattgtt ggcgcgtatt tatttgatcg agatggagag cgacgatcct
    17401 gctaacgctc tgccgtcgac ggacaaagcg gtcagcgata atgacgatat gatgattctg
    17461 gtcgtggatg atcatccgat taaccgacgt ctgctggcag atcagttggg atcgttgggc
    17521 tatcaatgta aaaccgcgaa tgatggtgtc gatgcgctta atgtacttag caagaatcat
    17581 attgatatcg tgcttagcga cgtcaacatg ccaaatatgg atggttaccg cttgacgcaa
    17641 cgcattcgtc agttgggact gacgttgccg gtaatcggag taactgctaa tgcgttggct
    17701 gaagagaagc agcggtgtct ggagtccggt atggacagct gcctgtcgaa gccggtaacg
    17761 ctggatgtga taaaacagac gctgacggta tatgccgaga gggtcaggaa atcgcgggaa
    17821 tcgtaggacg gataaggtgt ttatgtcgca tctggcattc agtgcaaatg ccagataaga
    17881 cactgacgcg tcttatctgg cctactttaa tgtaatctgt tgaaataatg ggaatcgtag
    17941 gccggataag gcgtttacgc cgcatccggc aatcggtgca aatgccagat aagacactaa
    18001 cgcgtcttat ctggcctaca ggtgattagt ctttatctgc cggacttaag gtcacagaag
    18061 agagataatt cagcagggcg atatcgttct cgacacccag cttcatcatc gcagatttct
    18121 tctggctact gatggtttta atactgcggt tcagcttttt agcgatctcg gtcaccagga
    18181 agccttccgc aaacaggcgc agaacttcac tctcttttgg cgagagacgc ttgtcaccgt
    18241 aaccaccagc actgattttt tccaacaggc gagaaacgct ttccggggta aatttcttcc
    18301 ctttctgcag cgcggcgaga gctttcggca gatcggtcgg tgcaccttgt ttcagcacga
    18361 tcccttcgat atccagatcc aataccgcac taagaatcgc cgggttgttg ttcatggtca
    18421 gaacaatgat cgacaggctt gggaaatggc gcttgatgta cttgattaag gtaatgccat
    18481 cgccgtactt atcgccaggc atggagagat cggtaatcaa cacatgcgca tccagtttcg
    18541 gcaggttgtt gatcagtgct gtagagtctt caaattcgcc gacaacattc acccactcaa
    18601 tttgctcaag tgatttgcga ataccgaaca agactatcgg atggtcatcg gcaataatta
    18661 cgttcatatt gttcatgtaa taggctacct tgctacagca agctcttgac ataactgtca
    18721 atgtcgctga tgtatttttc tattcctgga acatccttct cacgaatcag atgttccagc
    18781 gtttcacata actgcttgcc gggtaccaga tttagcatgg caaatacgcc tttaagacga
    18841 tgagccgttt gggctaacgc agcaaagtca ctggttgctg cttcagtata cagcctctta
    18901 acatcatccg gtactgtgtc tacaaagagc gcataatagc cgctggcatg gagttgcgca
    18961 ttttcatctc cgcccagagg cgattctgtc acctcttcct gcgccagttg cacttcaatt
    19021 aattgtaaga ccgcttcctg catagcgttg ctcatattga agttgacgca caattgacca
    19081 ggcccaattt cccgtacgcc agactcatca tcgcttaaaa gcaagccaga ggcagtaaga
    19141 ttagacggat tatccgttaa aaagatatca taatcttgac taattaatct ttcatcgggt
    19201 gtgatacagg ttgcacccca attttctaac tggcgagtga caatattccg aatttctgcc
    19261 gaagtaacat ccaccattac gcagacatca tccagtaaac gctcttcttc ctcttcaact
    19321 tccgggtcag ctgcgagcat tttgatatgc acagagtagc gtgtaccaag cccatcccgc
    19381 gttttgatgt ttaaatgacc gcccagttta cgtgccagtt gatcgctcag ccagaatgcc
    19441 agcgggtccg ccttgccata gcgatcgttt tgggtctggt tgataaacgg gaagtgcaaa
    19501 ttatccattt catgaatact tacgccttct cccgtgtcca gaatgcggaa cgtcaggcgg
    19561 tcttcggagg actcatcctg atcaacctca agggtgattt ttcccaattg cgttgaggtc
    19621 acggcatatt gcatcagtag cagcaaaata cgtcgtaagg catcgcgatc gccgcggcgc
    19681 atatcgtgtg ctttcagatg attgttaatc agcagttgca gacctttacg cttgatggca
    19741 ggcaacactg aaggcacaac ttcatcaatt aaatcctgca cggagaacag caccgtctca
    19801 cttttccagc tatcgtccgc aagcatgttc gctaactgta tttcatcgac cagccgcacc
    19861 agcacatctg cctgattcgc cagttgtttg ctttccgggg cgttgagttt agccgcgctc
    19921 tccgccaggg actgtgcagg ttctttcagc gcatcgccaa tgtttttcat aaaggtcatc
    19981 cggccctgct ggtttttctc atacagacgc tgcgcctgct tgagtttctt gtttaccagc
    20041 acttcgcgat cctgatcgcg aataatgaaa atttgtgtgc gcggcgcgac ctggctgcgg
    20101 aacatgcgga tctcatacag ctcgttattg atcgtcgcct gaataatccc ctgatgctgt
    20161 tccgccatgg tggtgatgtt ttgcagattc aaatgcggca gcaaatgatc ggcaattttg
    20221 ttacttatga cagtgcggtt cgattcctga tcgtgaacca gcaggccgag cggcagcagt
    20281 gagactatct cttcattgat tgcccgtaaa atgcgcaatt cgttattgac cgccgtgctg
    20341 gggacgtttt ctgtactgcg gctggagaaa tggcggaatg tggtatagcc aaataacgcc
    20401 agcgccagca aaccgatgtt cagcagcagt ggcagcagaa tgttttgcaa cgtatccagc
    20461 aataaggtgc cataaggaac ctgccagacc aggcgcatat cggtagagtt gagtgccgag
    20521 gagatttcaa tcttcgtact gttaaagtgg atactgacgc tatccgtccc ttctttctca
    20581 ttatcattgt ttcccgttgc cgtcgcgtct ggctcaaggc ggaaactgtc cagcggcata
    20641 cccggtggga tcaaatcatt aatcggcaga tcaaaagcca cgaccgttgc cagatgtcct
    20701 ggctggttaa aggtagtacg caaggtaaag taatgaccgt tctgccaggc caggcggcgc
    20761 atgttagaaa aactttcgcg ttcatcgagg gcgttggcct gttgcaacat ttctgcacga
    20821 cgtgaatcaa caatgtcact gacggtcgat tctttaaatc cggaggtgag atctttgagg
    20881 ggcagggttg agatcagcac cagactgtta tcctgaccat tcaggtaata catcgaccac
    20941 ggtacatttt ctgcgcccca caatgtgtcc agataggtgg acatccgttg agtcatctca
    21001 agcgttgagt tgtcgtgaga gccaaagatt aacgcttccg ttttgcggcg cggtttttcc
    21061 agatagtaga catcctgttt caggcgcgtc tcttgtaaac cttcgccgga ggagggggag
    21121 gtcgtcgcgg caatgttgtc gtagatctgc caggtcacgt aacgccaggt atcgacgcgc
    21181 ttttggatag cgtgggtaat gtcgacaatc tggtaacttt tatctttcag ccaggcgtta
    21241 acggcgcttt gtaccattac ccccatcgtc accagtaaca caatgatcaa cagtaaaaag
    21301 aagcgggtaa tgctccccgg taggagtgaa aagcgggtcg tggccgttgt ctctttctga
    21361 cgcattcgtg tttatgacct gttaaaactt cgcgaagtga tagggcagta taaagggtac
    21421 agtgtgaaat tccagactct tacctgattg atgattctct attctcccgt gatatttatc
    21481 atcctgcatt gggggaaata gatgtacaag cgccattttg ctgtacaaat aacctacaaa
    21541 aagcccaaca aaaataattg caaataaaca aagattgctg gaaattatgc tgatgttaat
    21601 tgtttgtgaa atagttaaca agcgttatag tttttctgtg gtagcacaga ataatgaaaa
    21661 gtgtgtaaag aagggtaaaa aaaaccggat gcgaggcatc cggttgaaat aggggtaaac
    21721 agacattcag aaatgaatga cggtaataaa taaagttaat gatga
//
LOCUS       DAZKIV010000074        21430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZKIV010000074 DAZKIV010000000
VERSION     DAZKIV010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.074"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..367
                     /locus_tag="ACHOT6_002692"
     CDS             71..367
                     /locus_tag="ACHOT6_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9947885.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            373..1158
                     /gene="bet"
                     /locus_tag="ACHOT6_002693"
     CDS             373..1158
                     /gene="bet"
                     /locus_tag="ACHOT6_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9947886.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNNETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1155..1835
                     /locus_tag="ACHOT6_002694"
     CDS             1155..1835
                     /locus_tag="ACHOT6_002694"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9947887.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVVERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            1832..2014
                     /locus_tag="ACHOT6_002695"
     CDS             1832..2014
                     /locus_tag="ACHOT6_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFP9947888.1"
                     /translation="MKHPHDNIRVGTITFVYSVTKRGWVFPGLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            1987..2178
                     /locus_tag="ACHOT6_002696"
     CDS             1987..2178
                     /locus_tag="ACHOT6_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFP9947889.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMVAKAEADERDQV"
     gene            2255..2470
                     /locus_tag="ACHOT6_002697"
     CDS             2255..2470
                     /locus_tag="ACHOT6_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9947890.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2569..2790
                     /locus_tag="ACHOT6_002698"
     CDS             2569..2790
                     /locus_tag="ACHOT6_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9947891.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            2787..3425
                     /locus_tag="ACHOT6_002699"
     CDS             2787..3425
                     /locus_tag="ACHOT6_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFP9947892.1"
                     /translation="MSEINSQALREAAEQAMHDDWGFDADLFHELVTPSIVLALLDER
                     ERNQQYIKRRDQENEDIALTVGKLRVELEAAENNLIDSECHVAELEEALRDKQALLEA
                     SEKRNAKLQSENAYIRNRYKELDLLIGKNILVMQAAIIEWQATGDAKSGLAWIYNTLF
                     GPGELPDESEKDAQAYFNRKYAPIDEKLMELHKWFWEQSEAERAAGIRIKGE"
     gene            3427..4182
                     /locus_tag="ACHOT6_002700"
     CDS             3427..4182
                     /locus_tag="ACHOT6_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947893.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHMNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAL"
     gene            4193..4318
                     /locus_tag="ACHOT6_002701"
                     /pseudo
     CDS             4193..4318
                     /locus_tag="ACHOT6_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            4316..5023
                     /locus_tag="ACHOT6_002702"
     CDS             4316..5023
                     /locus_tag="ACHOT6_002702"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFP9947894.1"
                     /translation="MRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEPDDLSEWAD
                     MHFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEPRLHIKEPGNSPVIPD
                     GLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSLPATPDGWI
                     SCSERMPEMGERQCYVLAADFKNNYPPNIPNTQVGVYGDWFNDGKPTWDDGDGEDLYL
                     KEVTHWMQLPEPPQEVK"
     gene            5111..5353
                     /locus_tag="ACHOT6_002703"
     CDS             5111..5353
                     /locus_tag="ACHOT6_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9947895.1"
                     /translation="MFALIQRGQIYTDRAGYPVVITRSTQHSVFFRRMDGRSGRVRIG
                     EFNNLFEHIDQQEYRKILAGTEQEMRLKKLRAMQRR"
     gene            5357..5491
                     /locus_tag="ACHOT6_002704"
     CDS             5357..5491
                     /locus_tag="ACHOT6_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9947896.1"
                     /translation="MHTAFEFWVRKTFGNRYDLTRDVDGFYCREVVKRMFDVWFHCRG
                     "
     gene            5510..5764
                     /gene="xisR"
                     /locus_tag="ACHOT6_002705"
     CDS             5510..5764
                     /gene="xisR"
                     /locus_tag="ACHOT6_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9947897.1"
                     /translation="MQTIIYQITPSKWCTERVLIASTGLKPGTIERARRKSWMQGKEY
                     RHYAVEGDPGHYSECLYNIEEIMRWIENQKQPGAKNASSG"
     gene            5798..7084
                     /locus_tag="ACHOT6_002706"
     CDS             5798..7084
                     /locus_tag="ACHOT6_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9947898.1"
                     /translation="MSNASYPTGVENHGGSLRIWFHYNGKRVRENLGVPDTAKNRKIA
                     GELRTSVCFAIRMGSFDYAAQFPNSPNLKHFGLGKREITVKALSEKWLDLKKIEICAN
                     ALNRYQSVIKNMLPMLGEKKLVSSITKEDLLFVRRDLLTGYQKLSNGKTSSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLAPLKKSKVKPDPLTRDEFIRFIEACRHQ
                     QTKNLWILAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRTAVHKCTFVFSPQVTKQQQLSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKAG"
     gene            7105..7806
                     /gene="mlrA"
                     /locus_tag="ACHOT6_002707"
     CDS             7105..7806
                     /gene="mlrA"
                     /locus_tag="ACHOT6_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFP9947899.1"
                     /translation="MSLPLIPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            7866..7973
                     /gene="yohO"
                     /locus_tag="ACHOT6_002708"
     CDS             7866..7973
                     /gene="yohO"
                     /locus_tag="ACHOT6_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFP9947900.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(7954..8685)
                     /gene="yehW"
                     /locus_tag="ACHOT6_002709"
     CDS             complement(7954..8685)
                     /gene="yehW"
                     /locus_tag="ACHOT6_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFP9947901.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(8690..9616)
                     /gene="yehX"
                     /locus_tag="ACHOT6_002710"
     CDS             complement(8690..9616)
                     /gene="yehX"
                     /locus_tag="ACHOT6_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFP9947902.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(9609..10766)
                     /gene="yehY"
                     /locus_tag="ACHOT6_002711"
     CDS             complement(9609..10766)
                     /gene="yehY"
                     /locus_tag="ACHOT6_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFP9947903.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGALLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(10773..11690)
                     /gene="osmF"
                     /locus_tag="ACHOT6_002712"
     CDS             complement(10773..11690)
                     /gene="osmF"
                     /locus_tag="ACHOT6_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFP9947904.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKIDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(11901..14198)
                     /gene="bglX"
                     /locus_tag="ACHOT6_002713"
     CDS             complement(11901..14198)
                     /gene="bglX"
                     /locus_tag="ACHOT6_002713"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFP9947905.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVNELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRD
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            14394..16109
                     /gene="dld"
                     /locus_tag="ACHOT6_002714"
     CDS             14394..16109
                     /gene="dld"
                     /locus_tag="ACHOT6_002714"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFP9947906.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDVVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(16147..17079)
                     /gene="pbpG"
                     /locus_tag="ACHOT6_002715"
     CDS             complement(16147..17079)
                     /gene="pbpG"
                     /locus_tag="ACHOT6_002715"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFP9947907.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(17253..17840)
                     /gene="yohC"
                     /locus_tag="ACHOT6_002716"
     CDS             complement(17253..17840)
                     /gene="yohC"
                     /locus_tag="ACHOT6_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFP9947908.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            18010..18588
                     /gene="yohD"
                     /locus_tag="ACHOT6_002717"
     CDS             18010..18588
                     /gene="yohD"
                     /locus_tag="ACHOT6_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFP9947909.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(18718..19479)
                     /gene="yohF"
                     /locus_tag="ACHOT6_002718"
     CDS             complement(18718..19479)
                     /gene="yohF"
                     /locus_tag="ACHOT6_002718"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9947910.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(19532..20968)
                     /gene="mdtQ"
                     /locus_tag="ACHOT6_002719"
     CDS             complement(19532..20968)
                     /gene="mdtQ"
                     /locus_tag="ACHOT6_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFP9947911.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALDGGYKR"
     gene            21192..21275
                     /gene="yohP"
                     /locus_tag="ACHOT6_002720"
     CDS             21192..21275
                     /gene="yohP"
                     /locus_tag="ACHOT6_002720"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFP9947912.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         5427 a         5615 c         5447 g         4941 t
ORIGIN      
        1 tttcctgttt tcctaatcag cctggcattt cgcgggcgat attttcacag ccattttcag
       61 gagttcagcc atgaacgctt attacattca ggatcgtctt gaggctcaga gctgggcgcg
      121 tcactaccag cagatcgccc gtgaagagaa agaggcagaa ctggcagacg acatggaaaa
      181 aggcctgccc cagcacctgt ttgaatcgct atgcatcgat catttgcaac gccacggggc
      241 cagcaaaaaa gccattaccc gtgcgtttga tgacgatgtt gagtttcagg agcgcatggc
      301 agaacacatc cggtacatgg ttgaaaccat tgctcaccat caggttgata ttgattcaga
      361 ggtataaaac ggatgagtac agcactcgca acgctggctg ggaagctggc tgaacgtgtc
      421 ggcatggatt ctgtcgaccc acaggaactg atcaccactc ttcgccagac ggcatttaaa
      481 ggcgatgcca gcgatgcgca gttcatcgca ttgttgatcg tcgccaacca gtacggcctt
      541 aatccgtgga cgaaagaaat ttacgccttc cctgacaagc agaacggcat cgttccggtg
      601 gtgggcgttg atggctggtc ccgtatcatc aatgaaaacc agcagtttga tggcatggac
      661 tttgagcagg acaatgaatc ctgtacatgc cggatttacc gcaaggaccg taatcatccg
      721 atctgcgtta ccgaatggat ggatgaatgc cgccgcgaac cattcaaaac ccgcgaaggc
      781 agagaaatca ccgggccgtg gcagtcgcat cccaaacgga tgttacgtca taaagccatg
      841 attcagtgtg cccgtctggc cttcggattt gctggtatct atgacaagga tgaagccgag
      901 cgcattgtcg aaaataccgc atacactgca gaacgtcagc cggaacgcga catcactccg
      961 gttaacaatg aaaccatgca ggagattaac actctgctga tcgccctgga taaaacatgg
     1021 gatgacgact tattgccgct ctgttcccag atatttcgcc gcgacattcg cgcatcgtca
     1081 gaactgacac aggccgaagc agtgaaagct cttggattcc tgaaacagaa agccactgag
     1141 cagaaggtgg cagcatgaca ccggacatta tcctgcagcg taccgggatc gatgtgagag
     1201 ctgtcgaaca gggggatgat gcatggcaca aattacggct cggcgtcatc accgcttcag
     1261 aagttcacaa cgtgatagca aagccccgct caggaaagaa gtggcctgac atgaaaatgt
     1321 cctacttcca caccctgctt gctgaggttt gcaccggtgt ggctccggaa gttaacgcta
     1381 aggctctggc ctggggaaaa cagtacgaga acgacgccag aaccctcttt gagttcactt
     1441 ccggcgttaa tgttactgaa tccccgatca tctatcgcga cgaaagtatg cgcaccgcct
     1501 gctctcccga tggtttatgc agtgacggta acggccttga gctgaaatgc ccgtttacct
     1561 cccgggattt catgaagttc cggctcggtg gtttcgaggc cataaaatcg gcttacatgg
     1621 cccaggtgca gtacagcatg tgggtgacgc gaaaagatgc ctggtacttt gccaactatg
     1681 acccacgaat gaagcgtgaa ggcctgcatt atgtcgtggt tgagcgggat gaaaattaca
     1741 tggcgagttt tgacgagatg gtgccggagt tcatcgaaaa aatggacgag gcactggctg
     1801 aaattggttt tgtatttggg gagcaatggc gatgaagcat cctcacgata atatccgcgt
     1861 aggcacgatc actttcgtct actccgttac aaagcgaggc tgggtatttc ccggcctttc
     1921 tgttatccaa aatccactga aagcccagcg gctggctgag gagataaata ataaacgagg
     1981 ggctgtatgc acaaagcatc tcctgttgag ttaagaacga gcattgagat ggcacatagc
     2041 cttgctcaaa ttggagtcag gtttgtgcca ataccagtag aaacagacga agaatttcat
     2101 acgttagccg catccctttc acaaaagctg gaaatgatgg tggcgaaagc agaagcagat
     2161 gagagagacc aggtatgaca accactgaat gcatttttct ggcagcgggc ttcatattct
     2221 gtgtgcttat gcttgccgac atgggacttg ttcaatgaca cctcagcaag aaaacgccct
     2281 tcgcagtatt gcccgtcagg ctaattctga aatcaaaaaa gccagacagc agtttccgga
     2341 taaaaacgtc gatgacattt gccgtagcgt actgaagaag caccgcgaaa cggtaacgct
     2401 gatgggattc acaccgactc atttaagtct ggcgatcggc atgttaaacg gcgtctttaa
     2461 ggagcgatga acatgaaaag caaaatcatc agggagctac aggctccttt tttattattc
     2521 gcattcaccc tcaagcgtat taaccaacaa ttcagggatt aatgaaagat ggcggacatc
     2581 attgattcag catcagaaat tgaagaatta cagcgcaata cagcaataaa aatgcgtcgt
     2641 ctgaactacc agactatatc cgccactcat tgttgtgagt gtggcgatcc catagatgaa
     2701 cgaagacgtc tggccgttca gggttgtcgg acttgtgcaa gttgccagga agatctggag
     2761 cttatcagta aacagagagg ttcgaagtga gcgaaattaa ctctcaggca ctgcgtgaag
     2821 cggcagagca ggcaatgcat gacgactggg gatttgatgc ggaccttttc catgagctgg
     2881 taacaccatc gattgtgctg gcgctgctgg atgaacggga aagaaaccag caatacatca
     2941 aacgccgcga ccaggagaac gaggatattg ctcttacggt agggaagctg cgtgttgagc
     3001 tggaagcagc agagaacaac cttattgata gtgaatgcca tgttgctgaa ctggaagaag
     3061 cgctacgcga taagcaggcg ttacttgaag cctcagaaaa gcgcaacgca aaattacaaa
     3121 gcgagaatgc atacatccgc aaccggtaca aagaactgga cctgttaatc gggaaaaaca
     3181 ttcttgtcat gcaggctgcg attatcgaat ggcaggcaac tggcgacgct aaaagcggac
     3241 tggcatggat ttataacaca ctgtttggcc caggcgaatt gccggacgaa tctgagaaag
     3301 atgctcaggc ctactttaat cgcaaatatg caccgattga cgaaaagctt atggagcttc
     3361 acaagtggtt ttgggaacaa agtgaagccg agcgcgccgc tggcattcgc atcaaaggag
     3421 agtgagatga ttcactacca cggtgggcct attactcctg atacgtgcgc aatgagagca
     3481 tggaaagggc gacatgcgtt tatcagtttt gcgcattcag gccagatcaa tctcgcggct
     3541 gaatactgtc agtcgttcgc gctggacaac ggtgcattca ccgcctggaa agcagctggc
     3601 aaaaacaaaa tcgactggag cgattactac gagtttgttg ctcgctggaa gaatcaccca
     3661 ggattcgatt ttgccattat cccggatgtt attgatggcg gagaggagga aaatgatgcg
     3721 cttctgaatg agtggcctca cggaaaacta gctggcgttc cagtgtggca catgaatgaa
     3781 agtgacgagc gatttattca tttgtgcaat gagtttccgc gagtggctat cggtagttgt
     3841 ggcgactatg acgtaaagcg cccaactctt gcggtagcca gaatgaaaga cctgattcgt
     3901 cacattgttg atgggcatgg tcagccggtt acgaaactac atggattgcg catgttaaat
     3961 ccgctgatat tcacaaaatt acccttagcc agcgcagata gtacgaacgt cgctcgaaac
     4021 atcggtattg ataaagcctg gtctggggct tatgcacctg caagtaaaga gacacgcgca
     4081 gcattaatgg tagaacggat tgaggcacac aatagccctg gttctcttgc gtattgtgaa
     4141 caacgcgacc gctttgaaat gcaattgcaa ctagcacttt aaggactaac aaatgaccac
     4201 tattaccaaa gaacgtattg aattgttcat taaaaatccg cttgaaaacg ggcttactcg
     4261 tggcgaacaa atggaactgg cacgaattgc actggcatca ctggaacgcg aactgattcg
     4321 ccacgagcat gccaaatggt ctgactccac atttggctgc gttggcccca ttggtccact
     4381 gaaacacctc tcaaaagagg ctctggaagc cgcagccgaa ccagacgatc tcagcgagtg
     4441 ggctgatatg catttcctgt tgtgggatgc acagcgccgt gctggcatca gcgatgctga
     4501 aattaccgct gctatggaag ataaattgaa gatcaacatg gagcgccagt ggcctgaacc
     4561 aaaagatggt gagcctcgct tgcacattaa agaacccggc aactctccgg taattccgga
     4621 tggtttatcc acggtatgcg ctgaggctta tcaggttgta ggagttatgg cagatgcgct
     4681 tggtgtattc ggtgatgcag cagtacagaa agttctggat aacctgtcac agcaaaaact
     4741 tgttcacaga gatgtgctgc cgttctcgct tccggcaact ccggatggtt ggataagctg
     4801 tagtgagcga atgcctgaaa tgggagagcg acaatgctat gtgttagcag ctgactttaa
     4861 aaacaactac ccaccaaaca tccccaacac tcaggtcggc gtatatggcg actggtttaa
     4921 tgatggcaag cccacttggg atgacggtga tggcgaagac ctgtatctca aagaggtaac
     4981 ccactggatg cagctaccag aaccgccgca ggaggtgaaa tgatgaattg gcctgaagca
     5041 ttcaccgcag ttggagttgc aatagcggtg gcatttattc tgtattcgct tttccgctgg
     5101 ggataaagga atgttcgctc tgattcaacg tggtcagata tacacggaca gagccggata
     5161 ccctgtggtg attactcgca gtactcagca ctcagtgttc tttcgacgca tggacgggcg
     5221 ctccggacgg gtacgcattg gtgagttcaa caacctgttc gaacatattg accaacagga
     5281 gtaccgcaaa attctggcgg gcactgagca ggaaatgcgc ctgaaaaaat tacgcgcaat
     5341 gcaacggagg tgatacatgc atacggcttt tgagttctgg gttcgcaaga cattcggcaa
     5401 tcgctacgac ctgacccgtg atgtcgacgg cttctactgc cgtgaagttg tgaaacgaat
     5461 gtttgacgtg tggttccact gccgtggatg aaagttttat gaggttggca tgcagacaat
     5521 catctatcag ataaccccca gcaaatggtg tacggagaga gtccttattg catcaacagg
     5581 gctaaagccc ggcaccatcg agcgggccag aagaaagtca tggatgcagg gaaaagaata
     5641 ccgccattac gctgtagaag gtgatcctgg gcattacagt gaatgcctgt acaacatcga
     5701 agaaattatg cgatggatcg aaaaccagaa acaaccaggt gccaaaaatg caagttccgg
     5761 ttaacctgtt aatgctcctg gacgtctggg aggtttaatg agtaacgcat catacccgac
     5821 aggcgttgaa aaccatggag gatcactccg tatatggttt cactataatg gcaaacgtgt
     5881 cagagaaaac ctcggtgttc ctgacacagc caaaaaccgg aagatcgctg gtgaacttcg
     5941 cacttccgtt tgttttgcaa tcagaatggg gagtttcgac tacgccgcgc agttccctaa
     6001 ttcccctaac ctgaaacact ttggtctggg aaaaagagag ataaccgtta aggcactttc
     6061 ggaaaaatgg ttggacctta agaaaattga gatttgtgcg aatgcactta accgttacca
     6121 gtcagtaatt aaaaacatgt taccaatgtt aggtgaaaaa aaactggttt catccataac
     6181 aaaagaggat ttacttttcg taaggagaga tttgttgacc ggttaccaaa agctttctaa
     6241 tggaaagact tcttccataa aagggcgctc agtggtcacg gtaaactact atatgacaac
     6301 catagctgga atgtttcaat ttgcaacaga taatggttat acctcaggaa acccatttaa
     6361 cggtctggct cccttaaaaa agtccaaggt aaaaccagat cctctcaccc gtgacgaatt
     6421 tattcgtttt attgaggctt gccgtcatca acaaacaaaa aacctgtgga ttctcgctgt
     6481 atacacgggt attcgtcacg gggagttggt atcgctggca tgggaagata tagaccttaa
     6541 agcaaggact ataaccatcc gtagaaatta tacaaaactt ggcgaattca ctccaccaaa
     6601 aaccgatgca ggcaccggaa ggacaattca tctggttcaa ccagctattg atgctcttaa
     6661 aagccaggcg gaaatgacca tgcttggaaa gcaacattct gtagaggtga agcagaggga
     6721 atatgggaga actgctgtgc ataaatgcac ttttgttttt agtcctcagg taacaaaaca
     6781 gcagcagttg tccggacctc actacaaggt tgactccatc agggagtcat ggacaagtat
     6841 cttaaaacgc gcaggtctga gacacagaaa atcgtaccaa tccaggcata cttatgcatg
     6901 ctggtcactt gccgcaggag ctaatcctag ttttatcgca agccagatgg gccacacaaa
     6961 cgcacaaatg gtattcaatg tttacggagc atggatgaaa gacaacaatc acgaacagat
     7021 agaactcctt aacaaaagac tatctgaaag tgtcccatgt atgccccata agaaagcagg
     7081 gtaaaataaa aacttgcaaa atcaattagt ttacccttaa tccctgtcac gttacgcgcg
     7141 tggcagaggc gttacggatt gctgaaaccg caacggacag acggcggtca tcgactgttc
     7201 aacgatgccg atattgaccg tatccgcgag atcaaacgct ggatcgacaa cggcgtgcag
     7261 gtcagcaaag ttaaaatgct gctcagtaat gaaaatgttg atgtgcagaa cggctggcgc
     7321 gatcagcaag aaacattact gacctacctg caaagcggca atctgcatag cctgcgaacg
     7381 tggatcaaag agcgcggtca ggattacccc gcccagacac tcaccacaca tctgtttatt
     7441 cctctgcgcc gacggcttca gtgccaacaa ccgactctcc aggcgctgct ggcgatcctc
     7501 gacggcgtac tgatcaacta catcgccatt tgtctggctt cggcacgtaa aaaacagggt
     7561 aaagatgcgc tggtggttgg ctggaatatt caggatacca cccgtctgtg gctggagggc
     7621 tggattgcca gtcaacaagg atggcgcatt gatgtcctcg cccactcgct caatcaacta
     7681 cgccctgaac tattcgaagg ccgtacattg ctggtgtggt gcggtgaaaa tcgaacctcc
     7741 gcccaacagc agcaactcac cagttggcaa gaacaaggcc atgatatttt cccactcggc
     7801 atttaatgat tcgttaacaa atgcgcttta ctgtacaatc ctttcgttaa cataaggagt
     7861 gcaatatgcg catagctaaa attggggtca tcgccctgtt cctgtttatg gcgttaggcg
     7921 gaattggtgg cgtcatgctc gcaggttata cctttatttt gcgtgctggc taagcgcctg
     7981 caccagcctt tcaaacagtc ggtctgcgat gatcgccgcc agtgccacca gtaacgcccc
     8041 ctggatcaca tacgcggtat taaatccgct aagcccgatg atgatgggcg tacccagcgt
     8101 gctggcccct accgttgagg cgatcgtcgc cgtaccaatg ttgataatca ccgaagttcg
     8161 cacgcccgcc agaatcaccg gagccgccag cggtagctcg accttacgca gtcgctgacc
     8221 tcgactcatt cccatacctt tcgcaacttc tgtcacgctg gcatcaatcg ctcccagccc
     8281 ggcaagtgtc gcctgcagga cgggtagcac accataaagg atcaaggcga taatcgctgg
     8341 ttgcagacca aacccaatca ccggaacggc aatcgccagc accgcgacag gcggaaaagt
     8401 ctgcccaacg gcggcaatag tttccaccag tgggcgaaat tccgcgcccc acgggcgagt
     8461 gacagcaatt ccggcaccag taccaatgat caccgcaaac aaactcgaaa ttcccaccag
     8521 ccagaaatga gccagtgcca gagctgcaaa actttcttgc tgataaacgg gtcgtggcag
     8581 ttgtgggaac aaggcagcaa acagcggctg gctgtaaggc agccagaaaa tcagcgccac
     8641 aaacagagca atgagccaga acagcggatc gcgcaacatc ttcatacgct tacgcctcct
     8701 ccagcagatc ctgaaaatgc agcgtgccgc aaggctggcc ctgcgtgttc accaccggca
     8761 gcacctcgca tccccgcgcg acaaacaggg agagcgcatc gcgtagcgtc atctcttctg
     8821 ccagtgcctc accttctgcc cgttcttcgc gacgcacgta atccgccaca ctacgtaacg
     8881 aaagcaggcg cacacccagt tcactacgtc caaaaaactg gcggacaaaa tcattcgccg
     8941 gacgagtcag catcgtcagc ggattcccct gctgcaccac ttcaccgtga tccatcaata
     9001 ccagatgttc tgccagcctt agcgcctcat caatatcatg agtgaccagc acaatagtgc
     9061 gccccagcaa acggtgaatg cgcgtcatct cttgttgcaa cgcgccgcgc gttaccgggt
     9121 ccagtgcgcc aaaaggttca tccatcagta agacttgcgg atcggcagcc agtgcgcgcg
     9181 ccactcccac acgttgctgc tgaccaccgg aaagctgatg cggataacgc tcacgcaaat
     9241 ttgactccag ccccagtagc gccattaatt cgtcgatacg atcgtcaatc cgcgcccgcg
     9301 accatttttg caattgcggc acggtagcga tgttttgcgc cacgctccag tgggggaaca
     9361 ggccaataga ttgaatggca tagcccatcc ggcggcgcaa ctccagtact ggcagcgagc
     9421 gaatttcttc tccggcaaag cggatcacgc cgctgtcatg ttccaccagg cggttaatca
     9481 ttttcagggt ggtggatttg ccggagccag atgtgccaat cagcaccgaa aaactccctt
     9541 cctgaaaatt gagattgaga tcgttaacgg ctttttgtgc gccaaacagt ttgctgacat
     9601 ggctaaattc aatcattacg tttcaccttc agcagtgcga taagcaaatc gaacagcgcg
     9661 tcggtaagca ccgccagaac aattaccggg atcaccccca gcaacactaa atcaatggcg
     9721 ctgcttagca gcccctggaa aaccagcgca ccaaaaccgc ctgcgccgat taacgccgca
     9781 atcaccgcca tacctacagt ttgcaccatc accacccgca ggctgcgcag aaataccggt
     9841 aacgccagtg gtaactgaac atgcaggaat cgctgcgccc cgctcatgcc catcgctctg
     9901 gcgctctcca gcacatcgcg cgggatctgg ttcaaaccga ctaccacgcc gcgcaccagc
     9961 ggcagcaagg catagagcac cagcgcaatc agtgcgggtg tcattccggt tcctgctatg
    10021 ccgagcttcc ccagccacgg aaaggccgtc accagcgcgg caagcggcgc aatcaacagg
    10081 ccaaagagcg ccaccgaagg cacggtctga atgacattga gcagagaaaa aattgccccc
    10141 tgccgagcag tggaaaagta gcaccagatg cccaacggca caccaatcac taacgcaggc
    10201 agcagcgcac caaacagcaa cgtcagatgt tgtgccagcg cgtcgtcaaa cacatcctga
    10261 cggttggcgt attctttcat tagtgagaga tcgttaagcg tgccggagta cagcaaccac
    10321 agcggaataa tggcaatctg catatgcaac aaccagcgcc acagcggatg cgtggagatt
    10381 cggcggatgg catcgctaca ggccagcaat gccagcgccg cagccagcca gaaaccactg
    10441 ccgaggctgg tacgcgccag cgcactgcca ttttgcgcca gttgtgtcgc cgcctttccc
    10501 gcgccccaca ccagcaatac gaagacgaat tgcgccagaa tgagtgcaca aatgctgcct
    10561 tttttaccgg gaataaaaca ggccgtcagc caggcgcaac caacgcccac cagcatccag
    10621 atcgtttgcg gccacagctg ccagagatga cgcccctcac ccgaaactaa acgattaggc
    10681 gcgtaactga taaacggcag cgccgctgcg attgccgtca gcaacagcag cagcgccaga
    10741 acaggattaa tacggaaata agtcacggga aattacttcg tccacccttt ttgtttcagg
    10801 tagtcggcag ccactttttt ggcatccagt ccttccacag caatgctggc attcagttgc
    10861 tgcaatgttt ttgcatcgag gctggcgaag actggctgta gccactgtgc catttgcgga
    10921 tactccttca acaccgactc acgcaccact ggtgcaggcg cgtagatagg ttgcacacct
    10981 tgcggatcgc ttaaggtttg cagccccagc gccgctaccg ggccgtcagt gccgtaagcc
    11041 attgcagcat taacgccaga ggtttgctgg gcagcggctt tgatcgtcac cgccgtgtcg
    11101 ccaccagcca gtgacagcaa ctgatcctga ccgagcttaa agccataggc tttttcaaac
    11161 gcgggtaacg catcggcgcg ttcgataaac tctgccgagg ctgccagttt gaaggtgccg
    11221 ccctctttca gataacgact caggtcggca agcgaagtga gtttgttttt ctctgccaca
    11281 tcctgacgca cggcgatagt ccaggtgtta tttgcaggcg cgggggtcag ccagattaac
    11341 ttgttgtgct ccgaatcgat ctttttgact ttctcgtaac cttgctgcgc gtttttccac
    11401 gctgcatcgt tttcatcttt aaagaagaaa gcgccattgc cggtatattc cggatagata
    11461 tccagttcac cggaagtaat cgccccgcgc accacaggag tcgttccgag ttgcacttta
    11521 ttcaccgtcg gtacgccgtg gctttccagc acctgcaaaa tgatattgcc gagtagcgca
    11581 ccttcggtat cgatttttga accgacttta acgggggaag ccgcctgtag cggcaggctc
    11641 acggctgcca acataaccag tgaacctgcc cagagcttta agagtggcat gatgctttcc
    11701 tcattctttt actgttgttt tcagcgaatt aagagaaaag catagttgat aatggcgggg
    11761 ttagcttgag cggaaggatt cagttgcaga atcagataaa tacttaagag gcatattcgg
    11821 tccggcgttt cctgccggat gcggcgcgag cgccttatcc ggcctacaaa gggcgcaaac
    11881 gtcgcgccct tactaaagca ttacagcaac tcaaactcgc ctttcttaac gcgtgcggaa
    11941 tcagtgccga taaatacatt gaacttgcca ggctcggcgt catatttcat ctgttgattc
    12001 cagaacttca gcgcctcaat atcgatcggg aagctgacag tctgagtttc gcccggtttc
    12061 agggtgattt tctcaaagcc tttcagctgt ttcacagggc gactcatgga agccgtcaca
    12121 tcctgcaagt acatctgcac taccgtggcc ccatcgcgct taccggtgtt cgtcacctgc
    12181 acgctggcag tcactttgcc gtcacgcttc atggtcggcg cagaaagttt cacatcagag
    12241 acggtgaaag tggtgtagct cagcccatag ccgaacggat acaacgcccc gttagcttca
    12301 tcaaaataac gcgaagtgta tttgttcggc ttgtcggcat tatacgggcg accggtattc
    12361 agatggctgt agtacaccgg gatctgcccg acagaacgcg ggaaggacat tggcagcttg
    12421 ccggacgggt tgtaatcgcc aaacaataca tcggcaattg cattaccgcc ttcagtcccc
    12481 gcaaaccagg tttccagaat cgcatcagcc tgctgatctt ctttcaccag cgccagcgga
    12541 cgcccgttca tcagcaccag caccagcggt ttaccggtgg ctttcagcgc cgcaatcaag
    12601 tcacgttggc tttgcggaat agtgatatcg gtccggctgg aggcttcgtg cgccatcccc
    12661 tgtgcttcac cgactacagc caccacaaca tcagattgtt tcgccgtctg caccgcttca
    12721 tcaatcatct cttgcggcga acgcggatcg actttgaccg cttcttcata ctgattcagg
    12781 aaatcgataa tgcctttgtc actggtaacg ttcgcccctt tggcatacag cactttaccg
    12841 ttttcaccga cggcattttt aatcccggtc agtacggtca cggattgatc ggcaacaccg
    12901 gctgcggacc agctgcccat cacgtcacgt ttactgtccg ccagtggccc aaccaccgca
    12961 atggtggccg attttttcag cggtaacgtt tcgagacggt ttttcagcaa caccaggctt
    13021 tcgcgcgcca cttcacgcgc ttctttacgg tgcaggcggc tttcagcatt ggtatccacc
    13081 gggtcagact ctttcggccc caaatggctg tatgggtcgt taaacaatcc catatcatat
    13141 ttaacgttca gtacatggcg ggcagcatcg tccagctctt ccatcgtcac tttgccggat
    13201 ttgatcaacc caggcagata cttcgagtaa tactcgtcgc tcatactcat gttgattccg
    13261 gatttcagcg ccacgcgcac cgcatcttcc gggtctgccg ccgtgccatg tttaatcagc
    13321 tctttgattg caccgtgatc ggaaacggtg atgcctttaa agccccactg gtcgcgcaga
    13381 acatctttca gcagccagga atcggaggtg gctggcgtgc cgttcagcga gttcagcgcc
    13441 accatcaccg cgccgctgcc tgcgtccagc cccgctttgt acggcggcat ataatcatta
    13501 aacaggcgct gcggactcat atcgacggtg ttgtactctt taccgccttc taccgcgccg
    13561 tatgcggcaa agtgtttgac gctggtcatc accgagtagc gatctgccgg gcttttaccc
    13621 tgcatcgctt ccaccatggt tttacccatt gttgaggtga gatacgtatc ttcgccaaaa
    13681 ccttcggaag cacgtcccca gcgcggatcg cgcgagacat cgaccatcgg tgcccaggtc
    13741 atattcaggc catcatctgc cgcttcataa gcagagacac gtccgaccgt tttcactgca
    13801 tcgaggttaa aagacgaggc cagaccgagg ctaatcggga acaccgtgcg ctgaccgtgc
    13861 agcacgtcgt aagcaaagaa aagaggaatt ttcaggcggc ttaattccat cacctgatcc
    13921 tgcatggcgc ggatatcctg acgggttacg gtgttgaaaa tcgccccaac ctgaccgtct
    13981 ttgatcatct cgcggatcgc ctctttcggg ttatccgggc cgacgctgat taagcgcagc
    14041 tgaccaattt tctcatcaac tgtcattttc ttaagcagtt cgttgacgaa cgcatcccgc
    14101 gcttcgggcg ttaatggatg gttgccgaat aaatcatccg ccagtgctgg ctgcagggcc
    14161 agactcaccg cgattcctac tgaacatagc catttcatat ggatttttct tcctctgttg
    14221 cccgacgagg cagcgaaaat ggtgcaaaaa ccgtagtttg ccataagcat gatggagaga
    14281 gaaaaagaat gctcagttta ttgtctgaat tttcaaaata ttcactcgct gaattgtcat
    14341 acaaggcgct attctagttt gtgatatttt ttcgccacca caaggagtgg aaaatgtctt
    14401 ccatgacaac aactgataat aaagcctttt tgaatgaact tgcccgtctg gtcggtcatt
    14461 cacacctgct caccgatccc gcaaaaacgg cccgctatcg caagggcttc cgttctggtc
    14521 agggcgacgc gctggctgtc gttttccctg gctcactact ggaattgtgg cgggtgctga
    14581 aagcctgcgt caccgccgac aaaattattc tgatgcaggc tgccaataca ggcctgaccg
    14641 aaggatcgac gccaaacggt aacgactatg atcgagatgt cgttatcatc agcaccctgc
    14701 gtctcgacaa gctgcacgtt cttggcaagg gcgaacaggt gctagcctat ccgggcacca
    14761 cgctctattc gctggaaaaa gccctcaaac cgctgggacg cgaaccgcac tcggtgattg
    14821 gatcgtcgtg tataggcgca tcggttatcg gcggtatttg taacaactcc ggcggctcgc
    14881 tggtgcaacg tggcccggcg tataccgaaa tgtcgttatt cgcgcgtata aatgaagacg
    14941 gcaaactgac gctggtgaac catctgggga ttgatctggg cgaaacgccg gagcagatcc
    15001 ttagcaagct ggatgatgat cgcatcaaag atgacgatgt gcgtcacgat ggtcgtcacg
    15061 cccacgatta tgactatgtc caccgcgttc gtgatattga agccgacacg cccgcacgtt
    15121 ataacgccga tccggatcgg ttatttgaat cttctggttg cgccgggaag ctggccgtct
    15181 ttgcggtacg tctagatacc ttcgaagcgg aaaaaaatca gcaggtgttt tatatcggca
    15241 ccaaccagcc ggaagtgctg accgaaatcc gccgccatat tctggctaac tttgaaaatc
    15301 tgccggttgc cggggaatat atgcaccggg atatctacga tattgcggaa aaatacggca
    15361 aagacacctt cctgatgatt gataagttag gcaccgacaa gatgccgttc ttctttaatc
    15421 tcaagggacg caccgatgcg atgctggaga aagtgaaatt cttccgtccg cattttactg
    15481 accgtgcgat gcaaaaattc ggtcacctgt tccccagcca tttaccgccg cgcatgaaaa
    15541 actggcgcga taaatacgag catcatctgc tgttaaaaat ggcgggcgat ggcgtcggtg
    15601 aagccaaatc gtggctggta gattatttca aacaggccga aggcgatttc tttgtctgta
    15661 cgccggagga aggcagcaaa gcgtttttac accgtttcgc cgctgcgggc gcagcaattc
    15721 gttatcaggc ggtgcattcc gatgaagtcg aagacattct ggcgttggat atcgctctgc
    15781 ggcgtaacga caccgagtgg tatgagcatt taccgccgga gatcgacagc cagctggtgc
    15841 acaagctcta ttacggccat tttatgtgct atgtcttcca tcaggattac atagtgaaaa
    15901 aaggcgtgga tgtgcatgcg ttaaaagaac agatgctgga actgctacag cagcgcggcg
    15961 cgcagtaccc tgccgagcat aacgtcggtc atttgtataa agcaccggag acgttgcaga
    16021 agttctatcg cgagaacgat ccgaccaaca gcatgaatcc ggggatcggt aaaaccagta
    16081 aacggaaaaa ctggcaggaa gtggagtaaa aattacggat ggcagagtat cgccatccga
    16141 attcacttaa tcgttctgtg ccgtctgccc cgccgccgcc atttgggcgg ctttttgttt
    16201 tttatagctc aacgctgctg ccggcacagg catcacttta ccggtttcaa tccaggtacg
    16261 caggcggctg gcatcggcaa aatgggtata tttgccaaac gcgtccatca ctaccagcgc
    16321 caccggttta ttattgataa ccgtacgcat caccagacaa tggcccgccg cattggtaaa
    16381 gccggttttg gttaactgaa tattccagtt atcgcgatac accagatgat tagtattgcg
    16441 gaacggcagc gtatacgtcg ggttagagaa ggttgccata tcttcccggg tagtacttaa
    16501 ctgcccgatc aacggatatt gtttgctggc aatgagcagt ttggttaagt cacgggcagt
    16561 tgaaacgtta tgcaccgaca atccggtagg ttcaacaaag cgcgtgttgt tcattccgag
    16621 cgatttcgct ttcgcattca ttgccttaat aaaggctttg taaccaccgg gataatggtg
    16681 cgcaaggctt gccgccgcgc ggttttctga agacatcagc gccagcaaca gcatatcttt
    16741 acggctgatt tcgctattca gtcgtacgcg cgaatagacc cctttcatct ccggcgtctg
    16801 gctgatatcc acttttagtt tttcatccag cggcagtcgt gcatccagca caaccatcgc
    16861 ggtcattaat ttgctgatag acgcaatcgg acgcaccaga tccgggtggt tcgaatagat
    16921 cactttgttg gtattcagat caacaatcat cgcgctaccg gaggcaattt ccggttgtga
    16981 agcggtggta gcggctgccg ttttcgcaac ggcctgcggt gccaaaggca cagccagcat
    17041 cagggccagg ctaaataaag aaactcgaaa tttcggcatg atgagcattc agatagtggt
    17101 tcacgcgcac gggttgcgca ccgccggagt aaggatttac tgaggctagc gacgccatca
    17161 taacgagcaa aaagtgcgat cgtcaaagga gaatcgtgag gaaatgctgc attgctgaca
    17221 tttacgccag caatgcaacg tcaaagaact ttctagaaca aacgataacc gtagccccat
    17281 agtataacgg ttagggcgag cagcacttcc agtaccagca cgccaatcgc cagcgtcgaa
    17341 ctagagaagc taaggccttc ctctttgttg atattcagga agctcggaat accaaggtaa
    17401 agcaggtagc cggtgtaaaa cagcgccacc gtgccgacca gcgcacacaa ccagaccagt
    17461 ggataaagcg ccaccagacc gcttaaaaac agcggagttg caacgtagcc cgcgaagacc
    17521 atgcagtgcg caagtgacgg acgctgcgga taattacgcg ccatccacca gatgacccgc
    17581 cccattaccg caaccccagc cagcataacg ccataaaaca ggacagccag cgccagtccg
    17641 gtaaaccagg ataacttcag gatagtgcca tcgccaaaat tccagccaat ctgtgtagtg
    17701 ccaatgaagg cgcaaatcac cggaatcgcc gccatcagca aaacgtggtg ggtgtaatgg
    17761 tgagaaatcg tttcgttttc gcgattaatc acctgcattt cacgatcggg atgggaaaac
    17821 agtccccaga catggctcat accgccccct tgttgtgagt tcatgaacct gacagttcaa
    17881 gtataagtca gcttgtgatt attttttgtt cgctcatgta atttgcaccg tctgatgtac
    17941 gtcctttttc agcgtgaatg atttccaggg tgtatgctta aaggcaacca aagggagaca
    18001 gactggccta tggatctcaa tacactcatc tcacaatatg gttatgccgc gctggtgatc
    18061 ggtagcctgg cggaaggtga aaccgtgact ttgctaggag gcgttgcggc acatcagggg
    18121 ctattaaagt tcccgctggt ggtactttct gtggcgcttg gcggcatgat tggcgaccag
    18181 gtgctttatc tgtgcgggcg gcggtttggc ggcaagctgt tacgccgttt ctcgaaacat
    18241 caggataaaa ttgagcgggc gcagaaactt atccaacgcc atccgtatct gtttgtcatt
    18301 ggtacgcgct ttatgtatgg ctttcgggtg attggcccga cgctgattgg tgccagccag
    18361 ctgccgccga aaatctttct gccgctgaat attctcggcg catttgcctg ggcgttgatt
    18421 tttaccacta ttggttacgc tggtggtcag gtgattgcgc cgtggttgca caatctcgac
    18481 cagcatttga agcactgggt ctggttgatt ctggtcgtgg ttctggtggt gggcgtgcgc
    18541 tggtggctga aacggcgcgg gaagaaaaag ccggatcatc aggcgtaaaa ccattgccct
    18601 gggtaaggcg ttcgcgccgc atccgacatc cggtgcacca tgcctgatgc gacgcttgtg
    18661 cgtcttatca tgcctaccaa aattttaccc attgaaataa ccacgcacct cactgcgcta
    18721 ttctgggttg aactgcggat tcgccaacat aaagccgcca tccactatca acgactgccc
    18781 ggtggtgtaa tttgcgccct ctgagcacag ccacgctacc aggctggcaa tctcatgcgt
    18841 tgcgccaaaa cgccgcagag gaatcgaagg ctccgcgtcg ggcttcacgt cgctgtcatc
    18901 catgccattc attggcgtgg cgatcgcccc aggcgcgact gcgttcacca aaatcttatg
    18961 cctgaccagc tccagcgcca tcgttttggt taacccgccg agcgcatgtt tagcggctgt
    19021 gtaagcgctg gcatccggca gcggcgtatg ttcatgtacc gacgtaatgt tgatgatgcg
    19081 accgccctgc ccttgtttca ccatctggcg ggccgcaatt tgtgagcata aaaatgcacc
    19141 atcgacatca acggtaaaaa tcttgcgcca ctcatcgaaa gccatatcaa gaaacggcgc
    19201 tttggtcatt gcgcccgcat tattcaccag cacatcaata cgccccaacc gttgaatgag
    19261 tttctccagc gcctgcgcac cttctggcag tttaccgaga tccagctgca cgatctccgc
    19321 acgtacgccg tggctaacta cctcacgcgc ggtatctttt gccccttctt catctgagtg
    19381 ccaggtaatg ccaatatcaa acccctgctg cgccagtaat aacgcgcact ctttgccgat
    19441 ccccgaatcg gaggcggtaa taatcgcaac ctgtgccatc gagttctcca cttaacgctg
    19501 aataaacgtt aagtatagaa ggcgcatatc atcagcgttt gtaacccccg tccaacgcac
    19561 cagtgagttg aatggaagca tccagccact gcccttgcaa taacaggcca ttggcccgct
    19621 cacgcagcgc ggggattctg gcttcgctga cgcgggaacc agcaatgatg cccgcgttaa
    19681 agcgcgcctg cgcaagacct accacacgca aggcatcgcg ctcaatttgc gcctgatgct
    19741 ggtttttctc cgcaagtgtc tgaacctgac tggctgcccg cgccacgtca ttcaccgctt
    19801 caaccaccgc tttgttgtag ctggcgatag acaagttgct ttcggctttt gcgatatcaa
    19861 gattggcgtt aagacgacca ctatcgaaaa tgggtagcgt caggcctgcc gtaacgccca
    19921 tttgctgcgc ggaatgacgg aacagatcgc ttaagtgcaa cgcatcctgt tgcaggaagg
    19981 ccatcaggtt gatgtcagga taaaatgccg cttttgccgc atcaatggtg cttagcgatg
    20041 actcaacgta ccagtgcgcc gcctgcaaat ctgcccgccg ggccagtaag gagtacccca
    20101 gttcatcagg aagctggctt gccactttcg gcaacgcgac cggtttaagc ttcaatgact
    20161 ttgtctggtt atttgtaagt gcgcttaacc gtgcctcaat aattttcatt ttccccgcga
    20221 catcgttgag ctgctgccgg gttttgctgg cattaatatc ggtttccaca ccttcaactg
    20281 aagaagtaat cccgttctga tatagctggc gatcggtcgc gataatggtg ttctgctctt
    20341 tttctatttg ctgcaagacc gtgtttaacg ccgcctgggt ttgccactcc cagtacaggc
    20401 gggctacgct gccagccagc aattggcggg tttgctcgcg ttccgccgcc cgtgctttaa
    20461 ccgtacccag gcgggcagta acctccgccc gattctttcc ccagatatcg agatgccagc
    20521 ccgccgttaa gccaaaagta ccgttggtgt accacgggcc ggtcgtacct gcggccggat
    20581 cgttcagagc aaacggcccc attaagcctt ctgccgacat tttttgccgc tccatatccg
    20641 ccgaaaagtc gatctgcgga ccatcctgag tggcaactgc cttcgcctgg gcttcagcta
    20701 gctgaatgcg ctgttcagcc acctgcatat ccggtgcgtt ctgtagtgca ttgttaatta
    20761 atgaagtgag ttgattatcg tgatactcca gccaccattg gctgtctggc caaccatttt
    20821 tcagcgccgt gggtaatgcg gtgtcaactt gtgcagcggg cgtttgctgg cttaacgcct
    20881 ggcgggtttc atgcataggc gcacacccgg ccagcatcag taacagcgga aaacaggcga
    20941 tggctggata aaaggaatca cgattcatgg gggaataatc aggtaagaaa aggtgcgcgg
    21001 agattaccgt gtgttgcgat atatttttta gtttcgcgtg gcaatacatc agtagcaata
    21061 aaacgacata tccagaaaaa tatagactaa gtaaatgata tcttccgatt tatcttaatc
    21121 gtttatggat aacggcaaag ggcttcgttt tttcctatac ttattcagca ctcacaaata
    21181 aaggaacgcc aatgaaaatt atactctggg ctgtattgat tattttcctg attgggctac
    21241 tggtggtgac tggcgtattt aagatgatat tttaaaaatc agatatggca tataagttgt
    21301 caggtcagga actagatatt ttgtgtactt gatttgcaaa cagctccggt gttggtggcg
    21361 tcaaaatcgc tgaagcgttc cccgaaggcc ggggcagccc acatggatgt gggctgaggg
    21421 cgcgtcttac
//
LOCUS       DAZKIV010000075        20959 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZKIV010000075 DAZKIV010000000
VERSION     DAZKIV010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.075"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(395..1486)
                     /gene="ychF"
                     /locus_tag="ACHOT6_002721"
     CDS             complement(395..1486)
                     /gene="ychF"
                     /locus_tag="ACHOT6_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFP9947913.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1603..2187)
                     /gene="pth"
                     /locus_tag="ACHOT6_002722"
     CDS             complement(1603..2187)
                     /gene="pth"
                     /locus_tag="ACHOT6_002722"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFP9947914.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVSFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2465..2743
                     /gene="ychH"
                     /locus_tag="ACHOT6_002723"
     CDS             2465..2743
                     /gene="ychH"
                     /locus_tag="ACHOT6_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFP9947915.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2798..4477)
                     /gene="dauA"
                     /locus_tag="ACHOT6_002724"
     CDS             complement(2798..4477)
                     /gene="dauA"
                     /locus_tag="ACHOT6_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFP9947916.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4602..5549)
                     /gene="prs"
                     /locus_tag="ACHOT6_002725"
     CDS             complement(4602..5549)
                     /gene="prs"
                     /locus_tag="ACHOT6_002725"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFP9947917.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5700..6551)
                     /gene="ispE"
                     /locus_tag="ACHOT6_002726"
     CDS             complement(5700..6551)
                     /gene="ispE"
                     /locus_tag="ACHOT6_002726"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFP9947918.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6551..7174)
                     /gene="lolB"
                     /locus_tag="ACHOT6_002727"
     CDS             complement(6551..7174)
                     /gene="lolB"
                     /locus_tag="ACHOT6_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFP9947919.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7388..8644
                     /gene="hemA"
                     /locus_tag="ACHOT6_002728"
     CDS             7388..8644
                     /gene="hemA"
                     /locus_tag="ACHOT6_002728"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFP9947920.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8686..9768
                     /gene="prfA"
                     /locus_tag="ACHOT6_002729"
     CDS             8686..9768
                     /gene="prfA"
                     /locus_tag="ACHOT6_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFP9947921.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9768..10601
                     /gene="prmC"
                     /locus_tag="ACHOT6_002730"
     CDS             9768..10601
                     /gene="prmC"
                     /locus_tag="ACHOT6_002730"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9947922.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10598..10990
                     /gene="sirB2"
                     /locus_tag="ACHOT6_002731"
     CDS             10598..10990
                     /gene="sirB2"
                     /locus_tag="ACHOT6_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFP9947923.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10994..11803
                     /gene="sirB1"
                     /locus_tag="ACHOT6_002732"
     CDS             10994..11803
                     /gene="sirB1"
                     /locus_tag="ACHOT6_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFP9947924.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11839..12693
                     /gene="kdsA"
                     /locus_tag="ACHOT6_002733"
     CDS             11839..12693
                     /gene="kdsA"
                     /locus_tag="ACHOT6_002733"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFP9947925.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12842..12949)
                     /locus_tag="ACHOT6_002734"
     CDS             complement(12842..12949)
                     /locus_tag="ACHOT6_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9947926.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13377..13484)
                     /locus_tag="ACHOT6_002735"
     CDS             complement(13377..13484)
                     /locus_tag="ACHOT6_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFP9947927.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(13889..14989)
                     /gene="chaA"
                     /locus_tag="ACHOT6_002736"
     CDS             complement(13889..14989)
                     /gene="chaA"
                     /locus_tag="ACHOT6_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFP9947928.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            15259..15489
                     /gene="chaB"
                     /locus_tag="ACHOT6_002737"
     CDS             15259..15489
                     /gene="chaB"
                     /locus_tag="ACHOT6_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFP9947929.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            15647..16342
                     /gene="chaC"
                     /locus_tag="ACHOT6_002738"
     CDS             15647..16342
                     /gene="chaC"
                     /locus_tag="ACHOT6_002738"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFP9947930.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(16386..16739)
                     /gene="ychN"
                     /locus_tag="ACHOT6_002739"
     CDS             complement(16386..16739)
                     /gene="ychN"
                     /locus_tag="ACHOT6_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFP9947931.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            16924..18318
                     /gene="ychO"
                     /locus_tag="ACHOT6_002740"
                     /gene_synonym="iatA"
     CDS             16924..18318
                     /gene="ychO"
                     /locus_tag="ACHOT6_002740"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFP9947932.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSHGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQICSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(18319..18969)
                     /gene="narL"
                     /locus_tag="ACHOT6_002741"
     CDS             complement(18319..18969)
                     /gene="narL"
                     /locus_tag="ACHOT6_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFP9947933.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18962..20758)
                     /gene="narX"
                     /locus_tag="ACHOT6_002742"
     CDS             complement(18962..20758)
                     /gene="narX"
                     /locus_tag="ACHOT6_002742"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFP9947934.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
BASE COUNT         5254 a         5241 c         5378 g         5086 t
ORIGIN      
        1 catttttgca ttactaataa gaaaaagcaa aaaacatcga tattcatttt tggtcaatgg
       61 gttttctggc ctatcgttgt tattgtgcta ctggttatca ttttcatctt gccagcatat
      121 tggagcgtga tcaattttga tcagctgtga aggtggctga tgtgtagtga tacaaaaact
      181 tacgttatat cactgctaat gagatatccg tcatctgaac acttatgtct ttaattattc
      241 tcgtggttca ctataggcaa taagcacaaa agtgtaggat gttacaagaa tgattaggac
      301 tcggtgaaat gaaaaatcca cgcaattgcg tggattttat atacttttgc gctcttcatg
      361 agatttagcg aaacctcatg agacaataaa ttaattagac gttgaaaagg aagttcatca
      421 catcgccatc tttcacgatg taatctttac cttctgcacg cattttgcct gcttctttcg
      481 cgccttgttc acctttgtaa gtgatgaaat cttcaaacga gatggtttgt gcacggataa
      541 agcctttttc aaaatcagta tggattttgc ccgctgcctg cggcgcggtt gctccaaccg
      601 gaatggtcca tgcacgcact tctttcaccc cagcggtgaa gtaagtttgc aggttcagca
      661 gcttataacc ggcacggatc acacggttca ggcccggctc ttccagccca agctcctgca
      721 taaactcgtc acgttcttcg tcgtccagtt cggcaatgtc tgcttcaaca gcagcacaaa
      781 ccggaaccac aacagaacct tctttcgccg cgatttcacg cacctggtca agatatgggt
      841 tgttttcaaa accgtcttcg ttgacgttgg cgatgtacat tgttggtttc agcgtcagga
      901 agctcaggta acgaatagcc gctttctctt cagcgcttaa atccagcgcg cgcagcatac
      961 ctgcattttc caactggggc aggcattttt ccaggaccgc cagctcagct ttcgcgtctt
     1021 tatcgccacc tttggctttc ttctgtacgc gatgaatcgc acgttcgcag gtgtcgaggt
     1081 ctgccagcgc cagttcggtg ttgataactt caatatcgtc agccgggtta actttgccgg
     1141 aaacgtgaat gatgttgtca ttttcaaagc agcgaacaac gtgaccgatc gcttcggttt
     1201 cacggatgtt ggtcaggaac tggttaccca gaccttcgcc tttcgatgcg ccttttacca
     1261 gaccggcgat atcgacaaat tccatggtcg tgggaagcgt acgctgcggt tttacgattt
     1321 cagccagttg atccaggcga ggatcaggca ttggtacgac gcctgtgttc ggctcaatgg
     1381 tgcagaatgg aaagttggcc gcttcaatac cggctttggt cagcgcgttg aacagggtag
     1441 atttcccgac gttgggcaaa ccgacgatac cgcatttgaa tcccatgatt taaatcacct
     1501 taatatctta ataatcaacc tgttattgct aacagattgc agaaatggaa ataactttgc
     1561 ctattataca cggcactcgg caaaaatgcc gcagacaacg acttattgcg ctttaaaggc
     1621 gtgcaatcgg ttcgttgctt tggtcaagcc atctgtaaac cacatttcag tacaacgcgc
     1681 cgcttcgtca atggcttcat caattaactt ctgttcacta acaggcggtt tgcctaacac
     1741 aaaactgaca actttatttt tatcgcccgg atgaccgatt ccgatgcgta aacggtgaaa
     1801 gttagggtta ttacccaatt tactgatgat gtctttcagt ccattgtgac caccatggcc
     1861 accgcccaat ttaaatttgg cgacgccagg aggcagatcc agttcgtcgt gggccaccag
     1921 aatttcgtcc ggattaatgc ggaaaaaact ggccatcgcc gcaacggctt tgccgctgag
     1981 attcataaat gtagtcggga ctaacaggcg gacatcttcg cctccaagag tgactcgcga
     2041 agtataacca aagaatttag cctcttcgcg cagcggagcg cgcaaacgct ctgccagtaa
     2101 gtcaacgaac caggcaccag cattatgtcg cgttgcggcg tattcagcac cggggttcgc
     2161 caggccgaca atcaatttaa tcgtcacgtt tttttgtcct gagtgtgtac ataactggcg
     2221 cgtagtttac tggttgcggc cccgcttgac aaaaaactgc gtatcaaatg cagataacgt
     2281 aataattgcc tgagtggact attagaaagt caaggtgttc aggcgtttat ttgtaaagtt
     2341 ttgttgaaat aagggttgta attgtgatca cgcccgcaca taacccacag ggtgttgtct
     2401 atactttaca cataaggaag aggggtattc cctgttacaa cccagaaagt tccggaggtg
     2461 acatatgaaa cgcaaaaacg cttcgttact cggtaacgtg ctcatggggt tgggtctggt
     2521 ggtaatggtg gtcggcgtgg ggtattcaat cctcaaccag ttaccacagt ttaatatgcc
     2581 ccagtatttc gcacatggtg cagtgctaag tattttcgtc ggtgccattc tctggctggc
     2641 gggtgcccgt gttggcgggc atgaacaggt gtgcgaccgt tactggtggg ttcgtcacta
     2701 tgacaaacgt tgccgccgta gcgataatcg ccgtcatagc taacaaataa tgcagtttgc
     2761 tgaccagtca gtttgcgctg actggtcaat ctcgtactta taaatccgcc atcgccgcgc
     2821 gacgattcgg gaagaacgcg aggcgtcccg ggatcggttg aatgcctgcg cgagccatag
     2881 tgcgcagtgg ctggaattcc acgttgcaca cgcgcagttc acatccttcg ggcagacgct
     2941 tcacaaaacg ctggaacgca tcaagaccac cagcatcaag taccggaacg gcatcccact
     3001 tcagaatcac aatccgtttg ccttcaagac gtgactccag gtccgtgaac aagccttcag
     3061 cagcagcaaa aaacagcggg ccaataacgc gcagtaccag aacatcgtct ggaacatcta
     3121 cgactaccgg tgccaggcga gtcatacgtg cgatacgacg cataaacagc agcgatgcca
     3181 gcacgatccc cacgctgatg gcaataacca tatcaaacag cacggtcagc gacatgcaca
     3241 gcagcatgac aatgatgtca tctttcggtg catgacgcag caagtcgacc actttatgcg
     3301 cttcactcat gttccacgcc accatcaaca gcagggctgc catagcggaa agcggcagcc
     3361 aggagagcag cggtgccagt accagcaggg caagaataac cagaatagag tggatcaccg
     3421 ctgagatagg ggaagttgcc ccggcacgga cgttagcggc agaacgcgcg atggcagctg
     3481 tagcggtaat accaccaaag aacggagcga taatattccc cagtccctgt ccaaccagtt
     3541 cgctgttcgc cttgtgtttc gtcccggtca taccatccag taccacggcg cagagcagag
     3601 attcgattgc gccgagcatt gccattgaga atgccgcagg cagcagtgtg cgaatagaat
     3661 cccaggttag cgtgaattct gaattaggca gatcccacgg cagcaccagt tgcggcagca
     3721 gttgcggaat accgttaccc tgagaaccat cggccaggac gtagtggaat tgcgaaccga
     3781 tggtagcaac atgtccgccg agcaggttaa caatccccat caccgcgcaa ccagccagca
     3841 aggccggaag gtgaccgggt aaacgaatgc ccagacgcgg ccaaaaaaca agaataccta
     3901 gcgtcacaat gccaatggca gcatcaccca cattaatggt cggcagcgcc ataaataatg
     3961 cgccgacttt ttgtagataa tgttccggga catgggccat ttgcagaccg agaaaatctt
     4021 taatctgcat ggtaccgatg gtgatcccga tacccgaggt gaaacctaag gtgacggaaa
     4081 ccggaatata ctcaatcagg cgaccaaagc gtgccagacc catcagaatc aaaaagatcc
     4141 ccgacagcaa ggtcgcaacc agcagtcctg ccagtccaaa ctgttgcgac acgggataga
     4201 gaattaccac aaatgccgca gtcggaccgg aaacgctaaa gcgtgaccca cccgtcagag
     4261 caatgacaat ccccgcaaca gctgcggtat ataaaccgta ctggggtgcc acaccactac
     4321 caatagccaa cgccatcgcc agcgggatag caataatccc gacggttatc ccggcaatca
     4381 ggtcacgggt aaaccgtgcg gcagtatatt tttctttcca gcaagcgtcg atcagagcgc
     4441 ggaaaggcat cacatgtgag gaaaatattt tgttcacaat aatgtttcat ccgtgagcgc
     4501 atcatctgtc aactaaatgg caggtgaagg aggcataggt catacaaatg gatattacag
     4561 acaaaaaaac ccgccgcagc gggtctttga gccgggttcg attagtgttc gaacatggca
     4621 gagatcgatt cttcgttgct gatacgacga atcgcttcgg ccagcatacc tgacagggtc
     4681 agagtacgca cgttcggcag tgatttgatt tcatcgctca gcggaatggt atcgcagaca
     4741 acgacttcat caattacaga gttacgcagg ttgttcgccg cgttgccaga gaagatcggg
     4801 tgagtcgcgt acgcaaatac acgtttagca ccacgttctt tcagagcttc agcagcttta
     4861 cacagcgtac cgccagtgtc gatcatatca tcgaccagta cgcagtcacg acctgcaacg
     4921 tcaccgatga tatgcatcac ctgggaaacg ttcgcacgcg gacgacgttt gtcgatgatt
     4981 gccatatcgg tatcgttcag cagcttagcg atagcgcggg cacgcacaac gccgccgatg
     5041 tccggagaaa ccacaattgg gttatccaga ttcagctgca gcatgtcttc cagcaggatc
     5101 gggctaccaa atacgttatc aaccggaacg tcgaagaaac cctgaatctg ttcagcgtgc
     5161 agatccactg tcagcacacg gtcaacaccg acgctggaga ggaagtctgc aaccactttc
     5221 gcagtgattg gtacacgagc ggaacggacg cgacggtcct ggcgcgcata gccaaagtag
     5281 gggataacag cggtgatacg acctgcggaa gcacgacgca gggcatcaac cataacgact
     5341 aattccatca ggttgtcgtt agtaggggca caagtggact ggatgatgaa aatatcacca
     5401 ccgcgtacat tttcattaat ttgtacgctg acttcgccat cgctaaagcg acctacagcg
     5461 gcgtcgccga gtgaagtgta caggcggttg gcaatacgtt gtgctagttc cggggtggcg
     5521 ttaccagcaa aaagcttcat atcaggcacg agaagaacct caggcatgcg tccattggtg
     5581 gaaagaatct gccgaaaact gtgcgggcca ggcatgatcc tttccaggcg gtgtattaaa
     5641 gagcgcgatg caacgtctgg aacaaggtga cgttgtcacc gaaactcagc ttgcccggct
     5701 taaagcatgg ctctgtgcaa tggggaaaga ttaacgcctt tcgccacaaa gccattgagc
     5761 cattccgggg cttgctctag cacctggcgg gcttcagact ctgtatcaaa ttcagcaaag
     5821 acacaggccc ctgtcccagt caggcgcgac ggggcgtatt ctaacagcca ggaaagcacc
     5881 gcatcaacct cgcgaaaacg ttttcttgcg ataacctcgc aatcattgct gaattcacat
     5941 tttagcaacg tttctattga cctttttggc gtattgcgcg ggagttcagg atctttaaaa
     6001 atcaccggag tcggaatact tacaccaggg tgcgccacca gataccactt ctctggcgga
     6061 tccaccggcg ttagtatttc accaacgcct tcggcaaacg cggcatgccc ccgaacaaag
     6121 acaggaacat ctgcgcccag cgtcagcccc atttccgcca gctcatccat gcttagcccg
     6181 cattgccaga gatgatttaa tgccaccagg accgtcgcgg cattggatga accaccgccg
     6241 agaccgccgc ccatcggcaa acgcttgtca atgctgatat tcgcaccgct tcccgtcgga
     6301 agacgcccgc tgtctgccgc agttttcatc aacaaccgcg ctgcgcgaac gatcaggtta
     6361 tcttcatgtt ccacgccttc aacgggcgtt aacagacgaa tatccccatc gtcacgaagc
     6421 tcaatgctga tggtgtcgcc gtaatcaaga aactgaaaca gcgtttgcag cgtgtggtaa
     6481 ccatccgcac gctgaccggt aatgtataaa aacagattaa gttttgccgg agagggccac
     6541 tgtgtccgca ttatttcact atccagttat ccatttttaa cttgatgcgt tgaccaccgt
     6601 cggtgagttc catattggct ggcatcgcag gttgcgtttt ggtgtcataa ccaccataaa
     6661 caaccttcca gtttttgcca ttctggctgt aggtaatttc gctcaggcgg tactggtcgt
     6721 ccagtttgta gtcggttgca tcacccggta aacctaaaat ccactggcgc aagctgttga
     6781 gcggaattgg cattccggtc aatttgccaa tcatctcttc ggcgtcatcg gcggtataac
     6841 gctgaccttt attgtcgact aactgcacgt tacccggttg agcattcagc tccagttccg
     6901 tgctgcccaa tgggttagtg agcagcagac ggtagcgatc ctggccggtt tgctgccaga
     6961 aaaagcgggc gtacactttt tgttggtcag aaatataagc gaacgcgccg cgagtctgat
     7021 actgattaag attgcgcacg tcttgctgat gctgacgcca ttgtggcgaa tccgggcttt
     7081 tgccaggacc tttgggcgtg gtaacggaac aggcagtaag cacaagagca gccagcggta
     7141 gcaggcggat aagacgaaaa tcgggcaggg gcatagtgat gacaagtcct tgagatacgt
     7201 tgcagttata acccttaatg ctagcgttac cgtccgctat cgtctatgtt caagttgtct
     7261 taattgccag aatctaacgg ctttcggcaa ttactccaaa agggggcgct ctcttttatt
     7321 gatcttacgc atcctgtatg atgcaagcag actaacccta tcaacgttgg tattatttcc
     7381 cgcagacatg acccttttag cactcggtat caaccataaa acggcacctg tatcgctgcg
     7441 agaacgtgta tcgttttcgc cggataagct cgatcaggcg cttgacagcc tgcttgcgca
     7501 gccgatggtg cagggcggcg tggtgctgtc gacgtgcaac cgcacggaac tttatcttag
     7561 cgttgaagag caggataacc tgcaagaggc gttaatccgc tggctttgcg attatcacaa
     7621 tcttaatgaa gaagatctgc gtaaaagcct ctactggcat caggataacg acgcggttag
     7681 ccatttaatg cgtgttgcca gcggcctgga ttcactggtt cttggggagc cgcagatcct
     7741 cggtcaggtt aaaaaagcgt ttgccgattc gcaaaaaggt catatgaagg ccagcgaact
     7801 ggaacgcatg ttccagaaat ctttctctgt agcgaaacgc gttcgcactg aaacagatat
     7861 cggtgccagt gctgtgtctg tcgcttttgc ggcttgtacg ctggcgcggc agatctttga
     7921 atcgctctct acggtcacag tgttgctggt aggcgcgggc gaaaccatcg agctggtagc
     7981 gcgtcatctg cgcgaacata aagtacagaa gatgattatc gccaaccgca ctcgcgaacg
     8041 tgcccaaatt ctggcagatg aagttggcgc ggaagtgatt gccctgagtg agatcgacga
     8101 acgtctgcgc gaagccgata tcatcatcag ttccaccgcc agcccgttac cgattatcgg
     8161 gaaaggcatg gtggagcgcg cattaaaaag ccgtcgcaac caaccaatgc tgttggtgga
     8221 tattgccgtt ccgcgcgatg ttgagccgga agttggcaaa ctggcgaatg cttatcttta
     8281 tagtgtggac gatctgcaaa gcatcatttc gcacaacctg gcgcagcgta aagccgcagc
     8341 ggttgaggcg gaaactattg tcgctcagga aaccagcgaa tttatggcgt ggctgcgagc
     8401 acaaagcgcc agcgaaacca ttcgcgagta tcgcagccag gcagagcacg ttcgcgatga
     8461 gttaaccgcc aaagcgttag cggcccttga gcagggcggc gacgcgcaag ccattatgca
     8521 ggatctggca tggaaactga ctaaccgctt gatccatgcg ccaacgaaat cacttcaaca
     8581 ggccgcccgt gacggggata acgaacgcct gaatattctg cgcgacagcc tcgggctgga
     8641 gtagcagtac atcattttct ttttttacag ggtgcattta cgcctatgaa gccttctatc
     8701 gttgccaaac tggaagccct gcatgaacgc catgaagaag ttcaggcgtt gctgggtgac
     8761 gcgcaaacta tcgccgacca ggaacgtttt cgcgcattat cacgcgaata tgcgcagtta
     8821 agtgatgttt cgcgctgttt taccgactgg caacaggttc aggaagatat cgaaaccgca
     8881 cagatgatgc tcgatgatcc tgaaatgcgt gagatggcgc aggatgaact gcgcgaagct
     8941 aaagaaaaaa gcgagcaact ggaacagcaa ttacaggttc tgttactgcc aaaagatcct
     9001 gatgacgaac gtaacgcctt cctcgaagtc cgagccggaa ccggcggcga cgaagcggcg
     9061 ctgttcgcag gcgatctgtt ccgtatgtac agccgttacg ccgaagcccg ccgctggcgg
     9121 gtagaaatca tgagcgccag cgagggtgaa catggtggtt ataaagagat catcgccaaa
     9181 attagcggtg atggtgtgta tggtcgtctg aaatttgaat ctggcggtca tcgcgtgcag
     9241 cgtgttcctg ctacggaatc gcagggtcgt attcatactt ctgcttgtac cgttgcggta
     9301 atgccagaac tgcctgacgc agaactgccg gacatcaacc cagcagattt acgcattgat
     9361 actttccgct cgtcaggggc gggtggtcag cacgttaaca ccaccgattc ggcaattcgt
     9421 attactcact tgccgaccgg gattgttgtt gaatgtcagg atgaacgttc acaacataaa
     9481 aacaaagcga aggcgctttc agtgctcggt gctcgcatcc acgctgctga aatggcaaaa
     9541 cgccaacagg ccgaagcgtc tacccgtcgt aacctgctgg ggagtggcga tcgcagcgac
     9601 cgtaaccgta cttacaattt cccgcagggg cgcgttaccg atcaccgcat caacctgacg
     9661 ctctaccgcc tggatgaagt gatggaaggt aagctggata tgctgattga accgattatc
     9721 caggaacatc aggccgacca actggcggcg ttgtccgagc aggaataatg gaatatcaac
     9781 actggttacg tgaagcaata agccaacttc aggcgagcga aagcccgcgg cgtgatgctg
     9841 aaatcctgct ggagcatgtt accggcagag ggcgtacttt tattctcgcc tttggtgaaa
     9901 cgcagctgac tgacgaacaa tgtcagcaac ttgatgcgct actgacacgt cgtcgcgatg
     9961 gtgaacccat tgctcattta accggggtgc gagaattctg gtcgttgccg ttatttgttt
    10021 cgccagcgac cttaattccg cgcccggata cggagtgtct ggtggagcag gcactggcgc
    10081 ggttgcctga acaaccttgc cgtattcttg atctcgggac gggtaccggg gcgattgcgc
    10141 ttgcgctggc tagcgagcgc ccggactgcg aaattatcgc tgtagatcgt atgcctgatg
    10201 ctgtctccct ggcacaacgt aatgcccagc atctggcgat caaaaatatc cacattctgc
    10261 aaagcgactg gtttagcgcg ctagccgggc agcagtttgc gatgattgtc agcaatccgc
    10321 cgtatattga cgagcaggac ccacatcttc aacaaggcga tgtccgcttt gagccgctca
    10381 ctgcgctggt tgcggcagac agtggaatgg cagacatcgt gcatatcatc gaacagtcgc
    10441 gtaacgcgct ggtatccggc ggctttctgc ttctggaaca tggctggcag cagggcgaag
    10501 cggtgcgaca agcatttatc ctcgcggggt atcatgacgt cgaaacctgc cgtgactatg
    10561 gtgataacga gcgcgtaacg ctcggccgct attatcaatg acaagttttt ctacactgct
    10621 tagtgttcat cttattagta tcgcgctttc tgttgggcta ttaaccttac gtttctggct
    10681 acgttatcag aagcatccac aggcatttgc tcgctggacg cgcattgtgc cgccggttgt
    10741 cgatacggtg ttattgttaa gcggcattgc gttgatggct aaagcgcaca tcctgccatt
    10801 ttccgggcag gcacagtggc tgactgaaaa gctgtttgga gttatcattt atatcgtttt
    10861 gggttttatt gcactcgatt atcgtcgtat gcacagtcag caggcgcgca ttattgcctt
    10921 cccgctggcg ttggtggtgc tgtacatcat cattaaactc gccaccacaa aagtaccgtt
    10981 actggggtaa gtcatgagat cgttagctga tttcgaattt aataaagcgc cattgtgcga
    11041 aggcatgatc ctggcttgcg aagcaatccg ccgcgatttt ccctcgcaag atgtttacga
    11101 cgaactggag cgtctcgtta gtctggcgaa ggaagaaatc agccagcttc tgcctttaga
    11161 agagcagctg gaaaaactga tcgcgctgtt ttacggcgac tggggattta aagcctcacg
    11221 cggcgtttat cgtctttccg atgcattatg gctggaccag gtgttaaaga atcgacaggg
    11281 cagtgcggta tcattaggtg cggttttatt atgggtcgcg aatcgtctcg atttgccgct
    11341 gctgccggtg attttcccta cgcagctgat attgcgcatt gaatgtccgg atggcgaaat
    11401 ttggctgatt aatcctttta acggtgaatc gttaagcgaa catatgctgg acgtatggtt
    11461 aaagggaaat atcagcccgt cggcggaact gttttatgaa gaccttgatg aagctgataa
    11521 cattgaggta atccgcaaat tgctggatac actcaaagcc tcgttgatgg aagaaaatca
    11581 gatggagctg gcgttacgca ccagcgaagc tttattacaa ttcaaccctg aagatcccta
    11641 tgaaattcgc gatcgcgggt tgatttatgc gcaactggat tgcgaacacg ttgcgttgaa
    11701 cgatttaagt tatttcgttg aacagtgtcc ggaagacccg atcagcgaaa tgatccgtgc
    11761 gcaaataaat aacatcgcgc ataaacatat tgtgctgcat taattaatcg acattttact
    11821 caagattaag gcgatcctat gaaacaaaaa gtggttagca ttggcgacat caacgtagca
    11881 aatgacctgc cgttcgtact gtttggcggt atgaacgtgc tggaatctcg cgatctggcg
    11941 atgcgcattt gcgagcacta cgtaactgtg acccagaaac tgggtatccc ttacgtgttc
    12001 aaagcctctt ttgacaaagc caaccgctcc tccatccact cttatcgtgg accgggcctg
    12061 gaagaaggga tgaaaatctt ccaggagttg aagcagactt ttggcgtgaa aattatcacc
    12121 gacgttcacg aaccaagtca ggcacagccc gttgctgatg tcgtggatgt gattcagttg
    12181 ccggcgtttc ttgctcgcca gactgacctg gttgaagcca tggcgaaaac cggtgcggta
    12241 attaacgtca agaaaccaca gtttgtcagc ccgggacaga tgggtaatat cgttgataaa
    12301 ttcaaagaag gcggcaacga aaaagtgatt ctttgcgatc gtggtgctaa cttcggctat
    12361 gacaacctgg ttgtcgatat gctgggcttc agcattatga agaaagtgtc tggtaactcg
    12421 ccggtgattt tcgacgtgac ccacgcactg caatgccgcg atccgtttgg cgcagcttcc
    12481 ggtggtcgtc gtgctcaggt ggctgagctg gcacgagccg gtatggcggt aggtctggcg
    12541 gggctgttta ttgaagcgca tccggatccg gaacatgcga aatgtgatgg tccatccgcg
    12601 ctgccgctgg ctaaactgga accgttcctc aagcagatga aagcgattga tgatctggtg
    12661 aaaggtttcg aagaactgga taccagcaag taatcttttt tgcttgaaaa ataaagtctt
    12721 agcgttctgc gttaagactt ttttcatggg tgccagatac aaaaaaggcc gcaggctgtt
    12781 acccctgcgg ccggtttcgg gcgcatattg ccatcacggc agcctgacgc ccgttttcac
    12841 cttacttccg gttacgccac cagccgacaa tcgctgcggt aataattccc gccaggatcg
    12901 gtgctgccag gtcgtgccag aaaatcatgg caaactgcgc gagcgtcata tagccgcctt
    12961 gttgtaatga caacattttg cggctattct tgaattgttc tggttcaaga ttagcccccg
    13021 ttctgttgtc aggttttacc tctcaacgtg cgggggtttt ctctttccag caaccaatgc
    13081 caccagggat aaagcccccg caacattgcg cctcaccgga taacgccggc ttgatgtgga
    13141 tactacgtct caattcatct tcacttcatc cctgaaatgt ttgcaatgaa gagtgcattc
    13201 cggtttttca acagctgtta cagtcgtttc atgagggctc tggatgatgc ttccagctcg
    13261 ggttgccaat atttacttgt ggaagagata aagacaaaaa tggccgcagg ctgttacccc
    13321 tgcggccggt ttcgggcgca tattgccatc acggcagcct gacgcccgtt ttcaccttac
    13381 ttccggttac gccaccagcc gacaatcgct gcggtaataa tccccgccag gatcggtgct
    13441 gccaggtcgt gccagaaagt catggcaaac tgcgcgagcg tcatatagcc gccttgttgt
    13501 aatgacaaca ttttgcggct attcttgaag tgtctggttt caagattagc ccccgttttg
    13561 ttgtcaggtt ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca
    13621 gggataaagc ccccgcaaca ttgcgcctca ccggataatt ccggcttggt gtggatacta
    13681 cggctcaatt catcttcact tcatccctga aatgtttgca ataaagagta cattccggct
    13741 tttcaacagc tgttgcagtg gtttcgtgca ggttgtggta caggctcgca attctgatta
    13801 acgagctgtc caggtagtat gaagaaatac aataagcagg aatttatctt atctcccccg
    13861 atttccgtca tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa
    13921 acagtgccag atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat
    13981 ggcacagcac taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgta
    14041 attcgttacc cgtcataaag gcaattagcg tgacgacagg tacggtaagc gaaatggttg
    14101 ctaacactga accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt
    14161 ttaacgcacc taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg
    14221 cgacaggggc attcatgctg tcgagcaatg tctccagcgg gctggcattc attttggtga
    14281 ctgcaataac ggcaatcaga tggataatca accagatagc atgccacacg ctgctatggg
    14341 cagacggttt accgtgatgc gggtcatcgt catcactgtc atcttcgtgc tcgtagacaa
    14401 acaaactttg atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa
    14461 ttaatgctac cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa
    14521 ataccagtac gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca
    14581 gattcatata ttgggtggca aacttacgac cgcccaacaa taatgaaaag ccaaccagcc
    14641 caccggtaac aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcgt
    14701 cgccggttgc cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa
    14761 gaataagcga accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa
    14821 cactaaaggc gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg
    14881 gtagtgtctg gctacttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa
    14941 gcgaagtctc cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc
    15001 ctttgcagat gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc
    15061 tggatatcgg taacatttta cgaattttta ccccgctgtc gattttttac tcattggggc
    15121 ataaaaataa gtattacgtt tagacaatgt ttgttttagc gtttcttaag aagagtctga
    15181 catgaaaatt cttatgtttt ggcaagagta gatattgttg accacactta atgttcaact
    15241 ttgtaaaagg agtcaacgat gccgtataaa acgaaaagcg atctgccgga aagcgtaaag
    15301 cacgttctac cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat
    15361 caatataaag ataaagacga tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa
    15421 gtggcctggg ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa
    15481 aaatcgtaaa accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc
    15541 cgtggattgc atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga
    15601 caccaaaaag ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga
    15661 tttcttgatg aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg
    15721 gtcagcagaa caacgggcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgagga
    15781 accggtgtgg atcttcggct atggatcgtt gatgtggaat ccggcactgg agtttaccga
    15841 atcgtgcacc ggtacactgg ttggatggca tcgcgcattt tgcctgcgcc tgaccgccgg
    15901 gcgcgggact gcgcaccagc cgggacggat gcttgcactg aaagagggcg gacgcaccac
    15961 aggcgtcgcc tatcgactgc cagaagagac gctggagcag gaactaacct tgttgtggaa
    16021 gcgagagatg attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg
    16081 acgcacagta aacgccattg tgtttattat ggatccgcga catccagaat atgaatctga
    16141 tactcgcgct caggtcatcg cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa
    16201 cgcacaatac ctgttttcac tggaacagga gcttatcaaa ctggggatgc aggacgatgg
    16261 gctgaatgat ttgctggtat cggtaaaaaa actgctggcg gagaattttc cggatggtgt
    16321 gttacgtccg ggattcgcct gagtaaactt cccgcatagt ggggcgtcag acgccccctc
    16381 aaacattaaa atgtgagcac tttatcggct gacagcgtcc attgcgccag ttccacaaga
    16441 gtaccgattt ccaccccatc aatcagagga agtgtactaa tcccgcgccc gtcggtacag
    16501 gttttgcaca atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg
    16561 ttgtagcctt cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatgagg
    16621 aacagacgca gatccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg
    16681 ttaaacaagg attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt
    16741 atttactcct gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt
    16801 ctgaccaatc ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag
    16861 ttgccagtaa tcgttattct ttaaggctat ggtttttcat tattaccgga agttaccgac
    16921 gttttgagcc gtttcgttcc tcgcattatc ccgttttatt tactcttgct tgtagcaggc
    16981 ggtacagcta acgcacaatc taccttcgag caaaaagcgg caaatccctt tgataataac
    17041 aatgatggtc tgccggattt aggcatggca cctgaaaatc atgatgggga aaaacacttt
    17101 gctgaaattg tgaaagattt cggcgaaacc agtatgaatg ataacgggct ggatactggc
    17161 gagcaggcaa aagctttcgc attgggaaaa gtccgcgacg cgcttagtca acaggttaat
    17221 cagcacgtag agtcctggct atcaccgtgg ggaaatgcca gtgttgacgt caaagtggat
    17281 aacgagggtc attttaccgg cagtcatgga agctggtttg tgccgttaca agataatgat
    17341 cgttatctca cctggagcca gcttggtctt actcagcagg atgatgggtt ggtgagcaat
    17401 gtgggcgttg ggcaacgctg ggcgcgcggc aactggctgg tgggttataa cactttttat
    17461 gacaacttgc tggacgaaaa tcttcagcga gcgggctttg gtgccgaagc gtggggcgaa
    17521 tatttgcgat tatcggcaaa cttttatcag ccatttgctg catggcatga acagacagcc
    17581 acgcaggaac aacggatggc gcgcgggtac gacctgacag cccggatgcg catgccgttc
    17641 tatcaacacc tcaataccag tgtcagcgta gaacagtatt ttggtgatcg tgttgatttg
    17701 tttaactctg gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattacacc
    17761 cctgtgccat tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgagaatcaa
    17821 aataacctcg ggctgaatct taattaccgc tttggtgtac cgctcaaaaa acaactttct
    17881 gcgggcgagg ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga
    17941 aataatctac cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca
    18001 ccgccgtggg atctaaaacc tggcgaaaca gtgccgctga aattacaaat ctgcagtcgt
    18061 tacggtattc ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc
    18121 gcacaagcca acagcgcgga gggctggacg ctgatcatgc ctgactggca gaacggggaa
    18181 ggggcaagca atcactggcg attgtctgtg gtggtggaag ataaccaggg gcagcgtgtc
    18241 tcctccaatg agatcacgct aacgcttgtc gaaccgttcg atgcattgtc aaacgacgaa
    18301 ctgcgctggg aaccgtaatc agaaaatgcg ctcctgatgc acccataccg ctgcttccac
    18361 gcgagacttg agcttcattt tcttcagcat gtgcttgacg tgcactttta ctgtgctttc
    18421 ggtgatgtcc aggcggcggg caatcatctt gttcggcaaa ccctgggcaa tcagcttgag
    18481 aatatcgcgc tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc
    18541 acgcaagctg gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc
    18601 tgcctgatgc aatgctttca gcagatcttc cggttccata tcttttaaca gatagccatc
    18661 cgcgccgcgt ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac
    18721 aatgcgccct gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc
    18781 gggcatattg agatctaaca ggatcagatc gggatcaaga gactccgcca gttcaatacc
    18841 ctgttcgcca ttactcgctt cgccaaccac ggtgatatct ggtgccatac tgataagctg
    18901 ttttacgcca gttcgcagca tcgggtgatc gtcaatcagc aggatagtag ccggttcctg
    18961 attactcatg ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca
    19021 ccacttcggt gccacctgat tcacgacggc ggacgcggca atcgcctcgt aaactttgcg
    19081 cacggtcgcg cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc
    19141 cgcagccgtt atcctgtacg gtcagtttga cctgattatc gttttgcgcc accgtcacca
    19201 cgacctcact cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca
    19261 acaagtggat tgcctgatgc gaaggcacca gacgaggcgg caattgataa tccagcttca
    19321 ccgggaagcc aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc
    19381 caggctcggt gagctgcaag cggaatgtgg tgagcaattc acgcaactgc gcccaggatg
    19441 cattcagttc gttacggatc tgacttaaca gttcgcggct gctttctggc agcgcatcgc
    19501 cctgcatctg taaacaactc acctgcatct tcatacaaga gagagattgg gcaatagaat
    19561 catgcagttc gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt
    19621 cctgatggcg atccagcgcc agcgtggcgg tgagttgttc aaccagggta tccaccagtt
    19681 gttgttgatc atggctaaga tgacgcccct gcggcagggt cgccagcaaa ataccgtact
    19741 gcgtatgaga gtcagccagc cgccacttca gggtcgtgcc gcgatcacca acgggtaata
    19801 cgccgcgcgg gcagagctgg cagcctttat catcacaagt catatctggc tggcaggtaa
    19861 actcctgatg attctcttca tcatcagtgt catacacccg caattcgata tcacgtagca
    19921 gggttaaatt ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc ggggcgcggg
    19981 aatgcaaacg gcggttagcc tgccataaaa aagagaggat ctgattttta tgctccagcc
    20041 cggcggtttt ctcctgaacc cgctgctcaa gtacggcata actttcggcc agttctgcag
    20101 acatattgtt caacgcagtt ccaagcatcg ccatttcgtt gcgcccgctg atgtttgcgc
    20161 gttgggtaaa atcgcgatga ctgacggcac tcgccattgc cagcagttgc cgccacggtt
    20221 gtagcagtcg cgcccgcaac cagataatag tgaacaccag taaaagtgcc ataaataccg
    20281 ccattacccg atggaccagt accactgtct cgatgcgcat ttccgtggtg cggtcaaaac
    20341 cagataccag ttgatcaagc ccggcaacaa actggctgac atccgctgac accgtttctc
    20401 ggttttgtgc acgcatcaac gcagggatca gttcattacg ccagtaatct tgtaaaccct
    20461 gtaattgcgc cagttgtccg tctcgttctg ctgctcgagt caactcggcg ctaaatgccg
    20521 tttgttccat ctctttaatt aagggcttgt ctttctcgct taatggcact gctgccaaca
    20581 gacggtaact ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa
    20641 cgccttgcac cagccagcca gaaaccgcca tccctgccag tccaatagca gtagaaagca
    20701 acacaataag cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt
    20761 aaacctcttc cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatacattct
    20821 ggaatggcag tattctcggc tattggctga agtataccca tacccggaaa gagttactcc
    20881 ttatttgccg tgtggttagt cgctttacat cggtaagggt agggattcta cagcaccgtg
    20941 aaaaatctca taattttta
//
LOCUS       DAZKIV010000076        20821 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZKIV010000076 DAZKIV010000000
VERSION     DAZKIV010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.076"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(207..854)
                     /gene="ychE"
                     /locus_tag="ACHOT6_002743"
     CDS             complement(207..854)
                     /gene="ychE"
                     /locus_tag="ACHOT6_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFP9947935.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(885..938)
                     /gene="ychE"
                     /locus_tag="ACHOT6_002744"
     CDS             complement(885..938)
                     /gene="ychE"
                     /locus_tag="ACHOT6_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFP9947936.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            1331..4006
                     /gene="adhE"
                     /locus_tag="ACHOT6_002745"
                     /gene_synonym="adhC"
     CDS             1331..4006
                     /gene="adhE"
                     /locus_tag="ACHOT6_002745"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFP9947937.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDAVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4130..5026
                     /locus_tag="ACHOT6_002746"
                     /pseudo
     CDS             4130..5026
                     /locus_tag="ACHOT6_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5308..5925)
                     /gene="tdk"
                     /locus_tag="ACHOT6_002747"
     CDS             complement(5308..5925)
                     /gene="tdk"
                     /locus_tag="ACHOT6_002747"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFP9947938.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVDSLTAIQERHRHD"
     gene            6530..6943
                     /gene="hns"
                     /locus_tag="ACHOT6_002748"
     CDS             6530..6943
                     /gene="hns"
                     /locus_tag="ACHOT6_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFP9947939.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7087..7995)
                     /gene="galU"
                     /locus_tag="ACHOT6_002749"
     CDS             complement(7087..7995)
                     /gene="galU"
                     /locus_tag="ACHOT6_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFP9947940.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8197..9210)
                     /gene="rssB"
                     /locus_tag="ACHOT6_002750"
     CDS             complement(8197..9210)
                     /gene="rssB"
                     /locus_tag="ACHOT6_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFP9947941.1"
                     /translation="MTQPLVGKQILIVEDEPVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9302..10207)
                     /gene="rssA"
                     /locus_tag="ACHOT6_002751"
     CDS             complement(9302..10207)
                     /gene="rssA"
                     /locus_tag="ACHOT6_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFP9947942.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVEKKMDELLPLVRTNI"
     gene            10320..10778
                     /gene="ychJ"
                     /locus_tag="ACHOT6_002752"
     CDS             10320..10778
                     /gene="ychJ"
                     /locus_tag="ACHOT6_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFP9947943.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELIAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10828..11670
                     /gene="purU"
                     /locus_tag="ACHOT6_002753"
     CDS             10828..11670
                     /gene="purU"
                     /locus_tag="ACHOT6_002753"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFP9947944.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11829..11913
                     /locus_tag="ACHOT6_002754"
     tRNA            11829..11913
                     /locus_tag="ACHOT6_002754"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11863..11865,aa:Tyr,seq:gta)
     gene            11961..12092
                     /locus_tag="ACHOT6_002755"
     ncRNA           11961..12092
                     /ncRNA_class="other"
                     /locus_tag="ACHOT6_002755"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12276..12953)
                     /gene="narI"
                     /locus_tag="ACHOT6_002756"
     CDS             complement(12276..12953)
                     /gene="narI"
                     /locus_tag="ACHOT6_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9947945.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12953..13663)
                     /gene="narJ"
                     /locus_tag="ACHOT6_002757"
     CDS             complement(12953..13663)
                     /gene="narJ"
                     /locus_tag="ACHOT6_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9947946.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13660..15198)
                     /gene="narH"
                     /locus_tag="ACHOT6_002758"
     CDS             complement(13660..15198)
                     /gene="narH"
                     /locus_tag="ACHOT6_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9947947.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15195..18938)
                     /gene="narG"
                     /locus_tag="ACHOT6_002759"
     CDS             complement(15195..18938)
                     /gene="narG"
                     /locus_tag="ACHOT6_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFP9947948.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(19331..20722)
                     /gene="narK"
                     /locus_tag="ACHOT6_002760"
     CDS             complement(19331..20722)
                     /gene="narK"
                     /locus_tag="ACHOT6_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFP9947949.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWTVYGRHSKK"
BASE COUNT         5340 a         5456 c         4982 g         5043 t
ORIGIN      
        1 aattattctt cagcaagccc aggattatga tgtggcgaaa ataacatgat ggtgaggaga
       61 agcagttaag gaaatcctta taactaactg taatttgaga agaatatttt tacctattcc
      121 ttttgctttt gataactgcg cgggaacaaa gtaaaaataa tgacgaagta aacggagcat
      181 ggcagctccg tttcatttga aaggaattaa ttaagcaggc cggggaaaat ccccttaata
      241 ccagtaacga taaattcaat ccccaatgcc atcagcaata gccccataat acgcgtaatc
      301 acgttgatgc cggtctggcg taaaacccgt accagccacg gtgccatgcg gaacaatccc
      361 cagcaacata aagcgaacaa tgcaatagcc acaaagaaac caaacagata gctaatgctg
      421 tgataacgcg taccccagac gatggtagaa ctgatcgccc ctggccccgc catcaacggc
      481 aacgccaatg gcaccacacc aatgctttca cgtaccgcgg tttctgattt ttcttgcttg
      541 ttctgtttat cctcgccaag cttgccgctg atcatcgaca tcgctatcgt caccaccagg
      601 atgcccccgg cgatacggaa cgaatcaatt gatataccaa aaagttgcag aatcgtgtcg
      661 ccgagaaaaa gcgagatcca caagataatg gccacagaca ggttggctgt aaggttagtt
      721 ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga taaagacggg aataatccct
      781 accgggttga ccagcgcaaa taacccgatg aaaaatttga agtaaacggg aaaatcaaaa
      841 aaggtctgaa tcacggttag ctccgaagca aaagccggat aatgttagcc ataaataagg
      901 ttgaaaagac gcgctgacaa tacgcctttt gacagcattt ttcacctcct aactacttaa
      961 aattgctatc attcgttatt gttatctagt tgtgcaaaac atgctaatgt agccaccaaa
     1021 tcatactaca atttattaac tgttagctat aatggcgaaa agcgatgctg aaaggtgtca
     1081 gctttgcaaa aatttgattt ggatcacgta atcagtaccc agaagtgagt aatcttgctt
     1141 acgccacctg gaagtgacgc attagagata ataactctaa tgtttaaact cttttagtaa
     1201 atcacagtga gtgtgagcgc gagtaagctt ttgattttca taggttaagc aaatcatcac
     1261 cgcactgact atactctcgt attcgagcag atgatttact aaaaaagttt aacattatca
     1321 ggagagcatt atggctgtta ctaatgtcgc tgaacttaac gcactcgtag agcgtgtaaa
     1381 aaaagcccag cgtgaatatg ccagtttcac tcaagagcaa gtagacaaaa tcttccgcgc
     1441 cgccgctctg gctgctgcag atgctcgaat cccactcgcg aaaatggccg ttgccgaatc
     1501 cggcatgggt atcgtcgaag ataaagtgat caaaaaccac tttgcttctg aatatatcta
     1561 caacgcctat aaagatgaaa aaacctgtgg tgttctgtct gaagacgaca cttttggtac
     1621 catcactatc gctgaaccaa tcggtattat ttgcggtatc gttccgacca ctaacccgac
     1681 ttcaactgct atcttcaaat cgctgatcag tctgaagacc cgtaacgcca ttatcttctc
     1741 cccgcacccg cgtgcaaaag atgccaccaa caaagcggct gatatcgttc tgcaggctgc
     1801 tatcgctgcc ggtgctccga aagatctgat cggctggatc gatcaacctt ctgttgaact
     1861 gtctaacgca ctgatgcacc acccagacat caacctgatc ctcgcgactg gtggtccggg
     1921 catggttaaa gccgcataca gctccggtaa accagctatc ggtgtaggcg cgggcaacac
     1981 tccagttgtt atcgatgaaa ctgctgatat caaacgtgca gttgcatctg tactgatgtc
     2041 caaaaccttc gacaacggcg taatctgtgc ttctgaacag tctgttgttg ttgttgacgc
     2101 tgtttatgac gctgtacgtg aacgttttgc aacccacggc ggctatctgt tgcagggtaa
     2161 agagctgaaa gctgttcagg atgttatcct gaaaaacggt gcgctgaacg cggctatcgt
     2221 tggtcagcca gcctataaaa ttgctgaact ggcaggcttc tctgtaccag aaaacaccaa
     2281 gattctgatc ggtgaagtga ccgttgttga tgaaagcgaa ccgttcgcac atgaaaaact
     2341 gtccccgact ctggcaatgt accgcgctaa agatttcgaa gacgcggtag aaaaagcaga
     2401 gaaactggtt gctatgggcg gtatcggtca tacctcttgc ctgtacactg accaggataa
     2461 ccaaccggct cgcgtttctt acttcggtca gaaaatgaaa acggctcgta tcctgattaa
     2521 caccccagcg tctcagggtg gtatcggtga cctgtataac ttcaaactcg caccttccct
     2581 gactctgggt tgtggttctt ggggtggtaa ctccatctct gaaaacgttg gtccgaaaca
     2641 cctgatcaac aagaaaaccg ttgctaagcg agctgaaaac atgttgtggc acaaacttcc
     2701 gaaatctatc tacttccgcc gtggctccct gccaatcgcg ctggatgaag tgattactga
     2761 tggccacaaa cgtgcgctca tcgtgactga ccgcttcctg ttcaacaatg gttatgctga
     2821 tcagatcact tccgtactga aagcagcagg cgttgaaact gaagtcttct tcgaagtaga
     2881 agcggacccg accctgagca tcgttcgtaa aggtgcagaa ctggcaaact ccttcaaacc
     2941 agacgtgatt atcgcgctgg gtggtggttc cccgatggac gccgcgaaga ttatgtgggt
     3001 tatgtacgaa catccggaaa ctcacttcga agagctggcg ctgcgcttta tggatatccg
     3061 taaacgtatc tacaagttcc cgaaaatggg cgtgaaagcg aaaatgatcg ctgtcaccac
     3121 cacttctggt acaggttctg aagtcactcc gtttgcggtt gtaactgacg acgctactgg
     3181 tcagaaatat ccgctggcag actatgcgct gactccggat atggcgattg tcgacgccaa
     3241 cctggttatg gacatgccga agtccctgtg tgctttcggt ggtctggacg cagtaactca
     3301 cgccatggaa gcttatgttt ctgtactggc atctgagttc tctgatggtc aggctctgca
     3361 ggcactgaaa ctgctgaaag aatatctgcc agcgtcctac cacgaagggt ctaaaaatcc
     3421 ggtagcgcgt gaacgtgttc acagtgcagc gactatcgcg ggtatcgcgt ttgcgaacgc
     3481 cttcctgggt gtatgtcact caatggcgca caaactgggt tcccagttcc atattccgca
     3541 cggtctggca aacgccctgc tgatttgtaa cgttattcgc tacaatgcga acgacaaccc
     3601 gaccaagcag actgcattca gccagtatga ccgtccgcag gctcgccgtc gttatgctga
     3661 aattgccgac cacttgggtc tgagcgcacc gggcgaccgt actgctgcta agatcgagaa
     3721 actgctggca tggctggaaa cgctgaaagc tgaactgggt attccgaaat ctatccgtga
     3781 agctggcgtt caggaagcag acttcctggc gaacgtggat aaactgtctg aagatgcatt
     3841 cgatgaccag tgcaccggcg ctaacccgcg ttacccgctg atctccgagc tgaaacagat
     3901 cctgctggat acctactacg gtcgtgatta tgtagaaggt gaaactgcag cgaaaaaaga
     3961 agccgctccg gctaaagctg agaaaaaagc gaaaaaatcc gcttaatcag tagcgctgtc
     4021 tggcaacata aacggcccct tctgggcaat gccgatcagt taaggattag ttgaccgctc
     4081 cttaaactga ggcactataa cggcttccac aacagggagc cgttttctta tgccacttct
     4141 caatgatctg ctcgatttca gtgaccatcc gcttatgcct ccgccctctg cacaactatt
     4201 tgcagaacac cttcccaccg agtggataca acactgcctg acgctttctg ctcatgcgac
     4261 cgttcgccgc cgtcgtttac cgggggacat ggttatctgg atggtggtgc aatgagccaa
     4321 ttaccgatgt tgttcgccgt ctgaacctga gcgcggatgg cgaagcgggg atgaacctgc
     4381 tggccctcag cgctgtcacc caggcgcgtc agcgcgtggg ggccgcccca gtggaatggc
     4441 tcttccgcca gaccgcacag acaggggcgc ggaacgttac ctgaaggatg actggcacgg
     4501 cctgcaactt tttgccattg atggcgcaca gttcaggaca cctgataaac ccgagctgcg
     4561 tgaatattat gggtctgcca acacatccac taagcggcag aacgcctacc cggttatgcg
     4621 tctggtggcc ttaatgaacc tgggaagtca cattctgctg aatgccgtga ccgcacctta
     4681 ccggcagagc gaaactgtgc tggcccactc catgctcgcc accatcccgg ataactctat
     4741 tacgttgttt gataaacttt tttacagcga agacctgctg ctgacgctga accagaaggg
     4801 ttgtaaccgc cactggctac tgcctgcgtg gaagaatatc gcatcagaaa tgatagagct
     4861 gggaaacacg gcgtcaccag ggaccatccc gaaacgactg gagcatcttc ggggtgctct
     4921 ggaagtggtg ttcataacaa aacgcccccg accatcaagg ccgaggtcgg ttaagatatc
     4981 aaaaacccgt tatcctgtga aacatagtgc cgctcctctt aagtgaacgg cattgccctt
     5041 ctggggccgt attttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
     5101 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
     5161 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
     5221 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
     5281 ctcaccctgc cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
     5341 taatgagtcg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
     5401 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
     5461 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
     5521 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
     5581 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
     5641 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
     5701 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
     5761 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
     5821 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
     5881 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
     5941 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
     6001 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     6061 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactgctggt aactattcac
     6121 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     6181 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     6241 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     6301 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     6361 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     6421 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     6481 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     6541 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     6601 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     6661 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     6721 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     6781 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     6841 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     6901 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     6961 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     7021 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     7081 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     7141 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     7201 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     7261 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     7321 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     7381 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     7441 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     7501 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     7561 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     7621 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     7681 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     7741 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     7801 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     7861 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     7921 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     7981 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     8041 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     8101 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     8161 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     8221 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     8281 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     8341 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     8401 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     8461 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     8521 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     8581 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     8641 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     8701 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     8761 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     8821 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     8881 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     8941 attttcggtg gcagatatca ccagaaccgg ggtctggtcg cctctgttac gtatatgctc
     9001 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     9061 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     9121 caatgaggaa aaccacgaat ccagaagcga gcgaaatacc ggctcatctt caacaatgag
     9181 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     9241 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     9301 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcttttcc actgccagct
     9361 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     9421 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     9481 ccagcacctg gatagaagtg gtcataatct ctgttgccgt tggtgctgtc accgcccgac
     9541 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggcaga gaatcaccat
     9601 tctcgctatt ttcttcactg acattaaagg agagcaaatc ttgttgcatt aaatgagcat
     9661 cgtgctgcag gtcgaccgct atcacaatat cagcacccaa tgcacgcgtg agcgaaatag
     9721 gaattgggtt aacgactgcc ccatcaacca gccagtagcc gttatgcgcg actggtgcca
     9781 tgagtcctgg aatactgcat gacgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     9841 ataattcacg cccagtactt aaattggtgg caacagccgc gaagcgacgg gaacaatttt
     9901 cgatctctgt ttccggcatt atctcgcggt attgattgaa gacacgctcg ccgcgcagta
     9961 acccaccgcg ctgccaggag agatccatca ggcgtaaaac atcccaataa ctgaaagagg
    10021 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
    10081 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
    10141 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
    10201 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
    10261 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
    10321 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
    10381 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
    10441 ttgtgatgca agacgcagat tatttaataa agacctggca tccatcttgt ggagcagcgg
    10501 cgttacgtgc cgaattgatt gccggatttg cgcatactga atggttggga ttgaccgtat
    10561 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
    10621 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
    10681 aatggtacta tattgacggt acacgtccgc agtttggccg caacgatccc tgcccttgtg
    10741 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
    10801 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
    10861 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
    10921 ttaaatatcg tgcagaacaa tgaatttgtt gatcaccgta ccggacgctt ttttatgcgc
    10981 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
    11041 ccagaaggct cggtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
    11101 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
    11161 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
    11221 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
    11281 atggcggacg ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
    11341 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
    11401 ttcctgccag cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
    11461 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
    11521 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
    11581 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
    11641 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
    11701 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
    11761 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
    11821 ttacccctgg tggggttccc gagcggccaa agggagcaga ctgtaaatct gccgtcatcg
    11881 acttcgaagg ttcgaatcct tcccccacca ccatcacttt tcaaaagtcc gtaaactctc
    11941 aaacgaatcc gcaattaaat attctgccca agcggggaag gatgagaagc ttcgaccaag
    12001 gttcgacttg agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg
    12061 cgcagcgctg agtaatcctt cccccaccac catcgcttcc ttaaataatc aaaacctgtt
    12121 actccgagct aattttgccc gatggcacca cgtttaccgg gcctacaata tcctcccctg
    12181 taggtcggat aagcgaagcg catccgacaa ctcaccccgc accgtgccca taaaaaaacc
    12241 ccgccgaagc agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg
    12301 tacttacgtg tcagatactc caccggtacg ctccagatgt gtaccagacg cgagaacggg
    12361 aacagcagga ataacgtcat ccccagcacc aggtgcagac ggaagataaa cgccacacca
    12421 tcgaggtgtt gagaagcgcc accgtggaag gtcaccaccg actgcgccca gccaaccagt
    12481 ttcatcatct cgctaccgtc catatgctga gcggagaacg gaatggtcaa caggcccagc
    12541 gcgcactgga taacgagcag cgacaggatc aggatatccg ctccggtagt ggttgcacgc
    12601 acgcgtgggc tgaacagacg acgcttcagc aacagcacgc cgccaatcag acacagcacg
    12661 ccgctggcac caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct
    12721 tcatacatcc agtgcggcgt cagcataccg aagaagtgac caacaaaaat ccccagaata
    12781 ccgatatgga acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac
    12841 gccgcgcgcc aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc
    12901 gccccggcta tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct
    12961 gtcctccggt ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat
    13021 gagcagtgat tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt
    13081 cccagacagc atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca attttttccg
    13141 ccactttgtc gctgtcgata gcggtattgg ccaatttcag cagcagatca aacatcacgg
    13201 cataacggct ttcacgctgt tgcagacgcg cgctcagcaa tgccagaatc ggcgcgatat
    13261 ctttcaaacc ttccacggct tcgctttgcg gcagctgcga caggtactcc agatacagcg
    13321 gcagatggtc cggtaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca
    13381 gcaggtccac catcgcctgg ccgcggtcac gggattcacc gtgaacatgt tcaaatagca
    13441 acagtgacgt ggcgcggcca cggtcgaaca gttcgctgta ctgggcctgg gcatcgagcg
    13501 gatccatcgc cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatct tctttcgaca
    13561 gatttttcga cgcagcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat
    13621 ccggatattc aaggagacgc gatacaatca cgagttcgat catggatgcg gctccgtttt
    13681 gctggtcaca tcgatggcat cgatacgacg gctgttgaac agattgaatt tggtatctga
    13741 accgtggcag ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg
    13801 tgccagttca cgatgactac tcggcaccac aaagcgatct tcgtagttag caatcgccag
    13861 ataacggtac atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt
    13921 atcaacttta ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt
    13981 cagtgcgcgc agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac
    14041 cggaatacgc aagctttcga cgtctggcag aatgccgttg ctacccagct cacccgcgtc
    14101 tgctgcagac tgaatcggag acagaggcgg cacgtaccag accatcggca gtgtgcgata
    14161 ttctggatgc agcggcagcg ccagtttcca ttccattgcc attttataaa ccggcgactg
    14221 ctgtgcggct tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt
    14281 cggatcgttc ggatcgagga acacctccag ctgacgctgg taaagatctt tctcgttctc
    14341 ggtgctggct gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa gataacggat
    14401 acgaccgaca caggtttctg agcacacggt cggctgaccc gcttcaatac gcggatagca
    14461 gaagatgcac ttctcagact taccgctctt ccagttgaag tagatttttt tgtacgggca
    14521 tccggtgatg cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc
    14581 ttcttcacgc ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca
    14641 gtgttcgcac aggcgcggca aatacatcat aaaggtgttt tcgaactggc tatacatcgc
    14701 cttctggatg ttgtcgaagt tcttgtcttt cgccagtttg tcaaactcac cacccaggtc
    14761 atcttcccag ttcggccctt tttcgatttt cgccatccgt tccccggtaa tcagcgaacg
    14821 cggacgggca atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc
    14881 aaaatcgaac ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt
    14941 acccagcaac atggcacggt tacccatgcg cggctgcagt ttgccgttga ttttacggat
    15001 ccagccgcct ttgtattttt cctggttttc ccagtcagtc gggaagccct gacccggctt
    15061 ggtttccacg ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt
    15121 acaggtaact gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac
    15181 ttgtgaacga attttcattt tacgctctcc tgtacctggt cattgccttc gccatctaac
    15241 cagtcaatgt tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg
    15301 ccgtagtagt taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc
    15361 ggcgtgatac gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca
    15421 ggcaggttaa cgatacgttc ctgcgcgtga tacatcatgg tcatccctgc cggaacacgc
    15481 tggctgacaa ccgcacgggc agtcagagca ccgttgctgt tgaatacttc aatccagtcg
    15541 ttatcggcga tacccagatc tttggcatcg gcttcactca accagaccac cggaccaccg
    15601 cgacctaaag tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg
    15661 tgcggcgtca ggaagttgag cgctttttcc gggttgccgt tggatttctg gcctatcact
    15721 tctttcaccg aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca
    15781 cgcatccact ggtgatcctg atacagttgc tgacgaccag agagcgtacg ccatgggatc
    15841 agctcgtgaa cgttggtgta accggcgttg taagaaacgt gttcatcttc cagaccagac
    15901 caggtcgggc tggagataat tttgcgcggc tgtgcctgaa tatcgcggaa gcggatcttc
    15961 tcgtcttctt tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca
    16021 gcccaggctt tcacggctac ctgaccgttg gtttccggtg ccagtgtcag gatcatctct
    16081 gccgcatcaa ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg
    16141 tagttgagct tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg
    16201 ccattaccga ttttctccat cagcgggccg atagaggtaa agcgttcgta agtcgccgga
    16261 taatcgcgct ctacgaccat aatgtgtggc gcggttttac ctgggatcag gtcgcactcg
    16321 ccttttttcc agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcatgctgg
    16381 ataggcagcg tgacgatgtc ggtttcttta cccagatggc cgacgcacac ttcggagaat
    16441 ttcttcgcga tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc
    16501 gccgcagaca gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg
    16561 taccaggtcg ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag
    16621 tccagcgtaa ccaccagatc cagcttgcct tccagaccat tgtcctgcca gtccacttct
    16681 tccggcttca cgccgccctg ttgccccaga tctttaccct ggataccgtg ctccgtcccc
    16741 agcaggtact tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc
    16801 cagatgaaca ggttgcgcgg gtggttttta ccgttttctg gttgttctgc cgcaaaacgg
    16861 atggaaccct ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttcc
    16921 gcttcgccag cgatagtcag cgggttagtg cctaactgcg gtgcagacgg cagccagccc
    16981 atgcgttccg cacggacgtt aaagtcgatc aagtgtccgg tatagcggga tttgtccgcc
    17041 atcggtgaca gcaactcttc cgccgtgacg gtttcataac gccactggct ggagtggtta
    17101 tagaaataag aagtgctgtt catgtgacgc gccggacgct gccagtcaag ggcaaacgcc
    17161 agcggctgcc agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagccg
    17221 cccccgctct gaccgacaca gccgcagaaa atcagcatgt tgatcagacc acgatagttc
    17281 atatcgaggt gataccagtg gttcagcccc gcaccgacga taatcatcga acgaccgtgc
    17341 gttttatcag cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg
    17401 ccggtaatct gctcggccca ggccggggta taagctttca catcgtcata gctggttgca
    17461 cagttaacgt cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag atcataaacg
    17521 gtggtcacca gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc
    17581 agcagcacgt tttccagttc cactttgttg aagtgttccg tgccgtcgcc accaaagtac
    17641 gggaagccta cctcagcgat ctcatcctgg ctacccagca ggctcagttg cagctcggtt
    17701 tcttcgccag ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg
    17761 aagccaatag aaccgttcgg cgcaaccatt tctccattgg tattaaaggc gacagttttc
    17821 cattccggat tgttttcctg gcccagcgca tcaaccagat cagcagcgcg cagcatacga
    17881 cctgcagcgt agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag
    17941 cgacgcacat agtcggtgaa atactggctt gggttgtcga ggtggaattc acgcagcatt
    18001 acgtggccca tcgccagcgc cattgccgca tcggtgccct gtttcggtgc cagccacaga
    18061 tcgcacagtt tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggtccctttg
    18121 taacgcactt cagtaaagaa gtgagcatcc ggggtacgcg tctgcggtac gtttgacccc
    18181 caggcgatga tgtagctgga gttgtaccag tcagcagatt ccggtacgtc agtttgctcg
    18241 ccccaggttt gcggagacgc aggaggcaag tcgcagtacc agtcgtagaa gcttaagcaa
    18301 gtaccgccaa tcagcgagag atagcgtgca cccgatgcgt aagaaaccat cgacattgcc
    18361 ggaattggcg agaaaccagc aacacggtcc gggccgtagt ttttgatggt gtaaacgtta
    18421 gatgcggcga tcagttcgtt cacctcctgc caggaagaac gaacaaatcc accgcgtcca
    18481 cgcgcctgct taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca
    18541 accggatcgc tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc
    18601 atcgggtatt tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg
    18661 cagccgcgag gttcatggtt tggcagatcc ggacgggtac gcggatagtc agtctgctgg
    18721 gtttcccagg tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag
    18781 tttaccccgt gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc
    18841 tcccagtcac ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc
    18901 tgtttgaagt agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct
    18961 gtcggccctc tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca
    19021 gcgtgggaat tgataacgat caagtgtgat ggggatgaaa aataaagtaa attcctaaga
    19081 taccccttta ggataattaa cagttaaagc ataacatgct gtaaattaag acgttttata
    19141 cctgtagtta tttaaagcat gaagattttt atgcagatgg gtattaagga gtattcccca
    19201 tgggtaacat attaatggct cttaatgaaa tccggcgaaa taagccatat aacattagta
    19261 tgttgtaact aaataacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa
    19321 atattaagat ttatttttta gaatgccgac catataccgt ccaggtaatc actacgcagg
    19381 cgatatagaa aatcaaaaat accttcattg cgccgactgg cgaacccgtt aatgccagcg
    19441 agctaccaaa cgctttcggg ataaagaagc caccaatagc gccaatcgca gagatgaaac
    19501 ccagcgccgc tgccgtgtcg gttgccgctt cacgcatcgc acgttcgtca gaaccccctt
    19561 ctgctttcac gcgatccatt gtcagtttac ggaagatcac tgaaatcatc tggaaagtgg
    19621 aaccactacc cagcccagct gtcaggaaca gcgccaggaa gaccgcgaag aacgccatga
    19681 agcttccgcc ctgcccgtca gtcggtaagg tcaggaacag caggccgctg aaaatcgcca
    19741 tcagaataaa gttcaccagc gtgacacgag ttccgcccag acggtcagat aatgcaccac
    19801 ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa agcgtattgc agaatctgaa
    19861 catccgggaa ctgcgttttt gacagcattg caaagcccgc ggagaagccg atgaaggagc
    19921 cgaaggttgc cagatacagc aggctcataa tccacagatg accccgtttg agtaccggca
    19981 actgctcctt gatggaggct ttcgaggtag caagatcgtt catgccaaac cacgccgcaa
    20041 tagtgaagat ggcaaggaac ggcacccata tccaggacgc attcgccaga tacagctcag
    20101 tcccatccgg ctgtttgacg ccctggctac caaatactgc gaaaatcgac agtgatacca
    20161 ccagcggagc aaccaactgc atgacgctga cgcccatgtt tcccagacca ccattcagcc
    20221 ccagcgcgcc accctgcttc tgtttcggaa agaagaagct gatgtttgcc atactggatg
    20281 cgaagttcgc gccagcaaag ccgcatagca gagagatgat gatgaagacg ctataaggcg
    20341 tggaagtatc ctgcacggca aaacccagcc agacgcaagg aataatcaga ataccggtgc
    20401 tgaacgccgt ccagcgacga ccaccgaaga taggaaccat aaaggagtat ggaacacgta
    20461 ataacgcgcc agaaaccgaa ggcagcgcag tcaacataaa tagctgatcg gtcgtaaaat
    20521 taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa caacatccat acgcaaaacg
    20581 ccagcagcag acagggaacg gaaatccaca ggttgcggct ggcaatacgt tgaccgcgtt
    20641 gttgccagaa cgcaggatct tccggtcgcc aatctgttat gacagctcca gtagcccttt
    20701 cgggggcaga tgagtgactc atagacacct ctgatactcg tttcgatgtc tgccacctta
    20761 gtgtctggag ctaaaggcaa tttgatgtaa atcaaacgat aagataactt tatctataat
    20821 t
//
LOCUS       DAZKIV010000077        20780 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZKIV010000077 DAZKIV010000000
VERSION     DAZKIV010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.077"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..1717)
                     /gene="actP"
                     /locus_tag="ACHOT6_002761"
     CDS             complement(68..1717)
                     /gene="actP"
                     /locus_tag="ACHOT6_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFP9947950.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1714..2028)
                     /gene="yjcH"
                     /locus_tag="ACHOT6_002762"
     CDS             complement(1714..2028)
                     /gene="yjcH"
                     /locus_tag="ACHOT6_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFP9947951.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2228..4186)
                     /gene="acs"
                     /locus_tag="ACHOT6_002763"
     CDS             complement(2228..4186)
                     /gene="acs"
                     /locus_tag="ACHOT6_002763"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFP9947952.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4579..6014
                     /gene="nrfA"
                     /locus_tag="ACHOT6_002764"
                     /pseudo
     CDS             4579..6014
                     /gene="nrfA"
                     /locus_tag="ACHOT6_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
     gene            6059..6625
                     /gene="nrfB"
                     /locus_tag="ACHOT6_002765"
     CDS             6059..6625
                     /gene="nrfB"
                     /locus_tag="ACHOT6_002765"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFP9947953.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6622..7293
                     /gene="nrfC"
                     /locus_tag="ACHOT6_002766"
     CDS             6622..7293
                     /gene="nrfC"
                     /locus_tag="ACHOT6_002766"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFP9947954.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7290..8246
                     /gene="nrfD"
                     /locus_tag="ACHOT6_002767"
     CDS             7290..8246
                     /gene="nrfD"
                     /locus_tag="ACHOT6_002767"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFP9947955.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPNSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPTVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8266..9984
                     /gene="nrfE"
                     /locus_tag="ACHOT6_002768"
     CDS             8266..9984
                     /gene="nrfE"
                     /locus_tag="ACHOT6_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFP9947956.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGAVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYQMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9977..10360
                     /gene="nrfF"
                     /locus_tag="ACHOT6_002769"
     CDS             9977..10360
                     /gene="nrfF"
                     /locus_tag="ACHOT6_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFP9947957.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10357..10953
                     /gene="nrfG"
                     /locus_tag="ACHOT6_002770"
     CDS             10357..10953
                     /gene="nrfG"
                     /locus_tag="ACHOT6_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFP9947958.1"
                     /translation="MKQPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASKQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11295..12608
                     /gene="gltP"
                     /locus_tag="ACHOT6_002771"
     CDS             11295..12608
                     /gene="gltP"
                     /locus_tag="ACHOT6_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFP9947959.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12686..13375)
                     /gene="yjcO"
                     /locus_tag="ACHOT6_002772"
     CDS             complement(12686..13375)
                     /gene="yjcO"
                     /locus_tag="ACHOT6_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFP9947960.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13469..15616)
                     /gene="fdhF"
                     /locus_tag="ACHOT6_002773"
     CDS             complement(13469..15616)
                     /gene="fdhF"
                     /locus_tag="ACHOT6_002773"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15197..15199),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFP9947961.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRNTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15814..17280)
                     /gene="mdtP"
                     /locus_tag="ACHOT6_002774"
     CDS             complement(15814..17280)
                     /gene="mdtP"
                     /locus_tag="ACHOT6_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFP9947962.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17277..19328)
                     /gene="mdtO"
                     /locus_tag="ACHOT6_002775"
     CDS             complement(17277..19328)
                     /gene="mdtO"
                     /locus_tag="ACHOT6_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFP9947963.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQTVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATMLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMAADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDHAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19328..20359)
                     /gene="mdtN"
                     /locus_tag="ACHOT6_002776"
     CDS             complement(19328..20359)
                     /gene="mdtN"
                     /locus_tag="ACHOT6_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFP9947964.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASTMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20378..20653)
                     /gene="ytcA"
                     /locus_tag="ACHOT6_002777"
     CDS             complement(20378..20653)
                     /gene="ytcA"
                     /locus_tag="ACHOT6_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFP9947965.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4677 a         5506 c         5699 g         4898 t
ORIGIN      
        1 gggaaaaggt gatgcattct ggagaggaag agtattcccc ggtcaggcga ccggggaagg
       61 ggagagatta atgtgcgcgg ccttgctcaa cgccaaagcc ggtctgggag cggataaact
      121 gtgcacggaa cagttcacgc tcgcgcgcac cttccgctga gttatcggtt gccgagaaga
      181 accagatgcc gaggaatgcc acgctgatag agaacagcgc cgggtattca tacgggaaga
      241 tggctttttc gtgaccaagg atctgtaccc aaatcgtcgg gccgaggatc atcagtacca
      301 ctgcggtaat cagccccagc cagccaccca tcatcgcgcc acgagtggtc agtttcgacc
      361 agtacataga aagcagaatg atcgggaagt tacagctcgc tgcgatggca aacgccaggc
      421 ctaccatgaa ggcgatgttc tgattctcaa acagcacgcc gaggataatg gcaatcacac
      481 ctaaaatcag cacggtgatt ttcgataccc gcagttcttc acgttcggtc gcgccttttt
      541 tgaagacgtt agcgtacagg tcatgcgaaa ccgctgatgc gcccgccagc gtcagaccag
      601 caaccaccgc aagaatggtg gcaaaagcaa ccgctgaaat aaaaccgagg aacaggttgc
      661 cgcccactgc attcgccagg tgaacggccg ccatgttgtt accaccaatc agatggcccg
      721 ccgcgtcttt atattccgga ttcgcaccaa ccagcatgat cgcgccgaag ccgataataa
      781 aggtcagaat atagaagtag cccataaacc cggtggcgta gaacacgctc ttacgtgctt
      841 cgcgggcatc gctgactgta aagaagcgca tcagaatgtg cggcaagccc gccgtaccaa
      901 acatcagtcc cagacccaga gagagcgcgg agatcggatc tttcaccagc ccgcccggct
      961 tcatgatgtc gacacctttc gggtgtaccg ccatcgcttc actgaacaga ttgttgaagc
     1021 taaagccgac gtgtttcatc accataaagg ccataaagct ggcaccgaac agcaacagca
     1081 cggctttgat aatctgtacc caagtggtcg ccagcatgcc gccgaacagg acgtacatca
     1141 tcatcagcac gccgaccagc accaccgcaa tgtgatagtt aaggccaaac agcagctcga
     1201 tcagtttacc tgcgcccacc atctgggcga taaggtaaag cgccaccacc accagagaac
     1261 cacaggccga aagaatacga atcggccctt gtttcagacg gtaagaggcc acatcggcaa
     1321 aggtgtagcg ccccaggtta cgcagacgtt cggcaatcag gaacaaaatg atcggccagc
     1381 ccaccaggaa gcccagcgag taaattaagc cgtcatagcc ggaggtaaac accagcgcgg
     1441 agatccccaa gaatgaggcg gcggacatat agtccccggc aatcgccagc ccgttctgga
     1501 agccagtgat attgccgcct gcggtgtagt agtcgctacg agaacgtacg cgttttgatg
     1561 cccagtaggt aatgccgagc gtaaacacga cgaaaatcag gaacataata atcgcctgcc
     1621 agttcgttgg ctggcgctct acggccccgc taatagcatc cgcggcgtta gctgcgaaag
     1681 ggagtgtggc ggcaagcgcc gtcagaactc ttttcatgat gcttgtacct catgcaggac
     1741 ttcattatta agacggtcga attcgccgtt cgcccgccag atgtagatac cggtgagaac
     1801 aaaggagatc acaatcactc caacaccaat tggaataccg cgtgtgacgc tggtgttcgg
     1861 attcagcggc gtgcccagcc agccgggcgc gaaggcgatc agtaaaataa agccgatata
     1921 aactgccagc ataataatcg acaggatggt ggcaaaccgt tgccgttttt cgactaactc
     1981 cctgaaatgc gcattgtctt ctatccgctg ataaatagtg ccattcatca cagattctcc
     2041 agaggtaatg tagggattgt tttaattccc gctcccttat gggagaaggt taacgctcgg
     2101 gtaacccttg ccgaatgtag gccggataag gcgtttacgc cgcatccggc aatcaatgcc
     2161 tgatgcgacg ctgtcgcgtc ttatcaggtc tacaaaccga taccgactcg catccggcaa
     2221 ttgtgggtta cgatggcatc gcgatagcct gcttctcttc aagcagcttc tcgactacgc
     2281 caggatcggc aagcgtcgag gtatcgccca ggttgctggt atcgcccgcc gcaattttgc
     2341 gcagaatacg gcgcataatt ttgccggagc gggttttagg cagggagtcg gtccagtgca
     2401 gcacgtctgg cgtcgccagc gggccaatct ctttacgcac ccagttgcgg acttctgcgt
     2461 acagttctgg tgacggttcc tccccgtgat taagcgtgac gtaggcgtag atcgcctgac
     2521 ctttaatatt gtgcggaata cctactacgg cggcttcggc aatcttcgga tgcgccacca
     2581 gcgccgactc aatctctgcc gtccccagac ggtgaccgga gacgttcagc acatcgtcca
     2641 cacgcccggt tatccagtaa tagccatctt catcgcgacg cgcgccgtcg ccgctgaaat
     2701 acatattttt gaaggtggag aagtaggtct gttcaaaacg ttcgtgatcg ccaaacagcg
     2761 tacgcgcctg acccggccag gagtcggtga tcaccaggct gccttcggta gcgccttcca
     2821 gcgggttacc ttcgttatcg accagcgccg gttgcacgcc gaagaacgga cgtgttgccg
     2881 aaccggcttt cagctcggta gcgccaggca gcggcgtgat catgaaaccg ccagtttcgg
     2941 tctgccacca ggtatcgacc accggacatt tctcgttgcc gatttttttc cagtaccact
     3001 cccacgcttc cgggttaatt ggctcgccca cggaaccgag aatgcgcagc gacgaacggt
     3061 cggtaccctc gatcgcttta tcgccttccg ccatcagcgc gcggatcgcc gtgggtgcgg
     3121 tatagagaat attgacctga tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc
     3181 agttgggtac gccttcaaac atcagcgtgg tcgcaccgca ggccagcggg ccgtacagca
     3241 agtaactgtg tccggtcacc cagcccacat cggcggtgca ccagtagata tcacccggat
     3301 gataatcaaa gacatattta aaggtcagtg ccgcgtacac cagataaccg ccggtagtat
     3361 gcagcacacc ttttggctta ccggtagaac cggaggtgta gagaataaac agcggatctt
     3421 cggcgttcat ctcttccgcc tggtgctgat cgctcgcttg ctcaactagg tcgtgccacc
     3481 acaggtcgcg cccttcctgc cagtcaattt tcccgccagt acgcttcagt accaccacat
     3541 gctctacgct ggtgacgttc gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa
     3601 tactgcgccc ggcacgcaca ccttcgtcgg aagtgatcac cagtcgtgag ttggaatcaa
     3661 taatgcgccc ggcaacggct tccggcgaga agccgccgaa aatcaccgaa tgcaccgcgc
     3721 caatgcgggc gcaggccagc atcgcaaccg cggcttccgg caccatcggc atataaatcg
     3781 ccaccacatc acctttttta atgcccagct cgagcagggt attggcgaag cggcagacgt
     3841 cgcggtgcag ctctttatag ctgatatgtt tgctctggct ggcgtcgtcg ccttcccaga
     3901 tgatggcggt gcgatcgccg ttttcttgca gatggcggtc aaggcagttt gccgccagat
     3961 tcagcgtgcc gtcctcgtac catttaatgg acacattacc gggggcaaag gaggtatttt
     4021 tcaccttctg gtacggtttg atccagtcga gaatttttcc ctgttcgccc cagaaggtat
     4081 caggtacgtt aatagattgt tgatacatcg cctcgtactg ctgagggttt atcaggcaac
     4141 ggtctgcgat gttggcagga atggtgtgtt tgtgaatttg gctcatgctt ttgttctcct
     4201 tgtaggatgt taataatatg tggcataagc gttaaatgta ggggtattgg cagttttgtt
     4261 tagtatttgg gcgacagatc acgcaaaagt agaattgtgc aaataaacgg cagggtaatt
     4321 tttgaaggtc accaacaaaa gttgattaat tctttgagga acatgcagtt atgcatgctg
     4381 ttgaaaaaga ggaagatact gactaactct aaagtggtat tttacatgta cttacaattg
     4441 attaaagaca acattcatag tgtggttatt tgttacacat aggggcgagc aatgtcatga
     4501 cagtgtaggt gcggttactg tcgtgaaaaa caataaaaac cgccattgca acaatggcgc
     4561 aattcggatg aagcccctat gacaaggata aaaataaacg cacgccgtat cttcagctta
     4621 ttgattcctt ttttcttttt cacttctgtt cacgctgaac aaacggcagc tcccgcaaaa
     4681 cctgtaactg tggaagcgaa gaatgaaacc tttgccccgc agcatcccga tcaatatctc
     4741 tcctggaaag ccacctcgga acagtcagag cgtgttgacg ccctggcgga agatccacgg
     4801 ctggtgatcc tgtgggcggg gtatcccttc tcgcgcgatt acaacaagcc gcgtggacat
     4861 gcctttgctg tgaccgatgt gcgtgaaacc ctgcgtaccg gtgcgccgaa aaacgctgaa
     4921 gatggtccgc taccgatggc atgctggagt tgtaaaagcc cggatgtggc gcgtctgatc
     4981 cagaaagacg gcgaagatgg ctacttccac ggtaagtggg cgcgcggcgg cccggaaatc
     5041 gtcaacaact taggttgtgc cgactgccat aataccgcct caccagagtt cgccaaaggc
     5101 aaaccggagt taaccctttc ccgtccgtat gcggctcgcg cgatggaagc cattggtaaa
     5161 ccttttgaga aagccggacg tttcgaccag caatcgatgg tttgcggtca gtgccatgtg
     5221 gagtattact tcgacggcaa aaacaaagcg gttaaattcc cgtgggatga cggcatgaaa
     5281 gtcgaaaata tggagcagta ttacgacaaa attgccttct ctgactggac taactccctg
     5341 tcgaaaacgc caatgctgaa agcgcagcac ccggaatatg aaacctggac agcgggcatt
     5401 cacggtaaaa caacgtgacc tgtatcgact gccatatgcc aaaagtgcag aacgccgaag
     5461 gcaaactcta taccgaccat aaaattggta atccgtttga taacttcgcc cagacttgtg
     5521 cgaactgcca tacccaggac aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcagt
     5581 cgattaacga cctgaaaatc aaggttgaag atcaactggt tcacgctcac ttcgaagcga
     5641 aagcggcgct ggatgcaggc gcgacggaag ccgaaatgaa gccgattcag gacgatatcc
     5701 gtcatgccca gtggcgttgg gatctggcga tcgcttccca cggtattcat atgcacgcac
     5761 cggaagaagg tctacggatg ctcggtacgg cgatggataa agcggcggat gcacgcacca
     5821 aactggcgcg cctgctggcg accaaaggca tcacccatga aatccagatc ccggatatct
     5881 cgaccaaaga gaaagcccag caggccattg gcctgaacat ggaacaaatc aaggccgaga
     5941 agcaggactt catcaaaacg gtgatcccgc agtgggacga acaggcacgt aaaaacggtc
     6001 tgttaagcca ataaccccgt tccgcctcgc aaggggcgga aaacacaatg gagtgaatat
     6061 gagcgtatta cgttcgttat taactgctgg ggtgctggcg tcgggcctgt tgtggagcct
     6121 gaacggaatt accgctaccc ctgcggcgca ggcatctggt gatcgctacg aagttaccca
     6181 gcagcgtaac ccggatgccg cctgtctgga ctgtcataaa ccagataccg aaggtatgca
     6241 tggcaaacat gcttccgtta tcaacccgaa taacaaattg ccggtcacct gcaccaactg
     6301 tcacggccag ccatcaccgc aacaccgcga aggggtgaaa gatgtgatgc gctttaacga
     6361 gccgatgtac aaggtcggag agcagaacag cgtctgtatg tcctgtcatc tgccggaaca
     6421 gttgcaaaaa gcgttctggc cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg
     6481 ccattccctg catccacagc aagatacgat gcagacgtta agtgacaaag gacggattaa
     6541 gatttgcgtc gattgccaca gcgatcagcg caccaatccg aactttaacc cggcgtctgt
     6601 tccgttgctt aaggagcagc catgacctgg tctcgtcgcc agtttctcac cggcgtcggc
     6661 gtgctggcag ccgtcagcgg caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat
     6721 ggggtgcgtt acggcatggt gcatgacgag tcgttatgca tcggctgtac cgcctgtatg
     6781 gatgcttgtc gggaagtgaa caaggtgccg gaaggcgtct cgcgcctgat gattattcgt
     6841 agcgagccgc agggcgaatt tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag
     6901 cactgcgatc atgcgccgtg cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc
     6961 gctagcggga ttgtcgatgt taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc
     7021 gcctgtccgt accgcgtgcg ctttatccat ccggtcacga agacggcgga caaatgcgat
     7081 ttctgccgta agaccaattt gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg
     7141 acgaaggcgc tgacgtttgg caatctggac gatcctaaca gtgagatttc gcaactgctg
     7201 cgccagaagc ctacttaccg ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc
     7261 gtaccgttta aatacgggga ggtgagccaa tgacgcagac ttccgcattt cattttgaat
     7321 cgctggtgtg ggactggccg attgccatct acctgttttt gattggtatt tctgccggtc
     7381 tggtgacgct ggccgtgctg ttacgtcgct tctacccgca ggcgggcggt gcagacagta
     7441 cgttgctgcg caccacgctg attgtcgggc cgggcgcggt gatcctcggt ctgttgatcc
     7501 tcgtcttcca cctgacaaga ccgtggacct tctggaagct gatgttccac tacagtttta
     7561 cctcggtgat gtcgatgggg gtgatgctgt ttcagctcta catggtggtg ctggtgctgt
     7621 ggctggcgaa aatctttgaa catgatttgc ttgccctgca acaacgctgg ttgccgaagc
     7681 tggggatcgt gcaaaaggtt ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacat
     7741 tgatgctggt gttggcggtg ttgttggggg cttataccgg ctttctgctg tcggcgctga
     7801 aatcgtatcc gttcctcaat aacccgatcc tgccggtgct gttcctcttc tccggcatct
     7861 cgtccggtgc ggcggtggcg ctgatcgcca tggcgatacg ccaacgcagt aacccgaatt
     7921 ccacggaagc gcagtttgta caccgtatgg aaatccccgt ggtatggggt gaaatcttcc
     7981 tgctggtggc gttttttgtc ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg
     8041 tggcggcatt aggtggcggt ttctggacgt ggtggttctg gcttggtgtc gccgggctgg
     8101 ggctgattgt gccaatgttg ctcaaaccgt gggtcaatcg cagttccggc attcctaccg
     8161 tgctggcggc gtgtggggcc agtctggtcg gcgtgttgat gctgcgcttt ttcattctct
     8221 acgccgggca gttaacggtg gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt
     8281 cctgaagtcg gttttctggc gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg
     8341 acggcctttg cgggagtgcg gttgcgctgg cctgccatga tgcgactcac ttgcatcggc
     8401 attctggcgc agttcgcgct cctgctgctc gcctttggcg tactgacgta ttgttttctc
     8461 atcagcgatt tctcggtcat ttatgtcgcc caacatagct acagcctgct gtcgtgggaa
     8521 ctcaaactgg cggcggtgtg gggcggccat gaaggttcgc tgctgctttg ggtgctgctg
     8581 ctttccgcct ggagcgcgct gtttgcctgg cattatcggc agcaaaccga tccgctattt
     8641 ccgctgacgc tagccgtttt atctctcatg ctcgccgcac tgctactgtt tgtagtgctg
     8701 tggtccgatc ccttcgtgcg gatatttcca ccagcaatcg aaggccgcga tctcaatccg
     8761 atgctgcaac atcccggtct tatctttcat ccaccgctgc tttaccttgg ctatggcggt
     8821 ttgatggtgg cggcgagcgt ggcgctggcg agtttactgc gcggcgagtt tgatggtgcc
     8881 tgcgccagaa tttgttggcg ctgggcacta cctggctgga gcgcattaac ggcggggatc
     8941 atcctcggtt cctggtgggc ctattgcgaa ctgggctggg gcggctggtg gttctgggat
     9001 ccggtggaaa acgcctcttt attaccctgg ctttctgcca ctgcgctgct gcacagtttg
     9061 tccctgacac gccagcggga gattttccgc cactggtcgc tgttgctggc gatagtaact
     9121 ctgatgctgt cgctactggg taccttaatt gtccgttctg gcattctggt ttcggttcat
     9181 gcgttcgcac tggataacgt ccgcgccgtg ccgttgttca gcctgtttgc actgattagc
     9241 cttgcgtctc tggctctgta tggctggcga gcgcgggacg gtggtgcggt ggtgcgtttt
     9301 tcggggttat cgcgggaaat gttaatcctc gctacgctgt tgctgttttg cgcagtgcta
     9361 ctgatcgtgc tggtgggaac gctttatcag atgatttacg gtctgctggg ctggggacgc
     9421 ctctccgttg gcgcgccgta ttttaaccgc gcgacgttac cgtttggcct gttgatgctg
     9481 gtggtgattg tgctggcgac gtttgtctct ggcaaacgcg tgcagcttcc ggcgctggta
     9541 gctcatgcgg gcgtgctgtt atttgccgcg gggatcgtgg tttccagcgt cagccgtcag
     9601 gagatcagcc tgaatttaca gccgggtcag caggtgacgc tggcaggata caccttccgt
     9661 tttgagcgcc tcgatctgca agccaaaggc aattacacca gcgaaaaagc gatagtggca
     9721 ctgtttgacc atcagcaacg cattggtgaa ctgatgccgg agcggcgttt ttacgaagca
     9781 cgtcgtcagc aaatgatgga accgtcaatt cgctggaacg gcatccatga ctggtatgcg
     9841 gtcatgggtg aaaaaaccgg agcggatcgt tacgcttttc gtttgtatgt acaaagcggt
     9901 gtgcgctgga tctggggggg aggattgttg atgattgcgg gcgcattgtt aagcggatgg
     9961 cgggggaaga agcgcgatga ataaagggct tctcacgcta ctgctgttat ttacctgttt
    10021 tgcgcgcgct caggtcgtag acacctggca attcgccaat ccgcaacaac agcaacaggc
    10081 gttaaatatt gccagccagt tacgttgtcc gcagtgccag aatcaaaact tactggaatc
    10141 caacgcgccg gtggcagtca gtatgcgcca tcaggtttac agcatggtgg cggaggggaa
    10201 gaacgaagtc gaaatcattg gctggatgac cgaacgctac ggagattttg ttcgctataa
    10261 cccaccgtta acgggtcaga cgctggtgtt gtgggcgctg ccagtggtgt tgttactgct
    10321 gatggcactg atcctctggc gagtgagggc gaagcgatga aacagcccaa aataccggtg
    10381 aaaatgctga caacactcac gatattgatg gtatttctct gtgtcggcag ttatctgtta
    10441 agcccaaaat ggcaggcggt acgtgcggag tatcagcgtc agcgcgatcc gctacatcag
    10501 tttgccagca agcaaacccc ggaagcgcag cttcaggcat tacaggataa aatccgcgct
    10561 aatccacaaa acagcgaaca gtgggcgtta ctgggcgagt attatctgtg gcaaaacgat
    10621 tacagcaatt cgctgctggc gtatcgtcag gcgttgcaac tacgtggtga gaacgctgaa
    10681 ctgtatgcgg cgctggcgac ggtgctttat taccaggcca gccagcatat gaccgcccag
    10741 actcgcgcaa tgatcgataa agccctcgcg ctggactcta acgaaatcac cgctctgatg
    10801 ctgctggctt ccgatgcgtt tatgcaggcg aattacgcgc aagccatcga actatggcaa
    10861 aaagtgatgg atctcaactc accccggatc aaccggacac agctggtcga gtcgattaat
    10921 atggcgaaat tgttgcagcg gagatcagat taatcatctg gtgatgaaga ttatgttatt
    10981 gagttgtata aaaaataatc gaatgaacat atgccaaaaa taatcaccaa tcagtattat
    11041 tgcagattaa acaaataaaa atctttccat aacaaatggt tattcattaa tcctgccata
    11101 aactcgcgtt attatgcatt aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca
    11161 taccgcgcaa atgatactga tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa
    11221 ttcatgccct ggcagtatgt cacgttctcg cgtttctgaa cggggaacgg cgctccattg
    11281 aggaagttat tctgatgaaa agtataaaat tcagcctggc ctggcagatt ctgtttgcta
    11341 tggtgctggg cattctcctg ggaagctacc tgcactacca tagcgacagc cgcgactggc
    11401 tggtcgtcaa tttgctctct ccggcgggtg atatcttcat ccatctgatc aaaatgattg
    11461 ttgtgccgat tgtgatctcc acgctggtgg tgggtatcgc gggtgttggt gatgccaaac
    11521 agctcgggcg tattggcgcg aaaaccatta tttacttcga ggtgatcacc accgtcgcca
    11581 tcattttggg gatcactctg gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc
    11641 agctggcgac cgtcgatatc tcgaaatatc agagcactac ggaagcggta caaagcagtt
    11701 cccacggcat tatgggcacg attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg
    11761 cgaaaggcga aatgctgccg atcatcttct tctcggtgct gtttggtctg gggctttcct
    11821 ccctgcccgc gacgcatcgt gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca
    11881 tgtttaaagt gactcacatg gtgatgcgtt atgcaccggt gggtgtgttt gcgctgattg
    11941 cggtgacggt ggctaacttt ggtttctctt ctctgtggcc actggcgaaa ctggtgctgc
    12001 tggtgcattt cgccattctg ttcttcgcgc tggtagtgct gggaattgtg gcgcgcctgt
    12061 gcgggttaag cgtctggatc ctgattcgta ttctgaaaga tgagctgatt ctggcgtact
    12121 ccactgccag ctctgaaagc gtactgccgc gaattattga gaagatggaa gcctacggag
    12181 caccggcgtc gatcaccagc ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt
    12241 cgacgctgta tcaaagtatt gccgctattt tcatcgctca gctgtatggc attgacctgt
    12301 ccatctggca ggaaatcatt ctggtgctga cgctaatggt gacctcgaaa gggattgctg
    12361 gcgtgccggg cgtgtcgttt gtggtgctgc tggcaacgct gggtagcgta ggtattccgc
    12421 tggaaggcct ggcgtttatt gctggtgttg accgtatcct cgacatggcg cgtactgcgc
    12481 tgaacgtggt gggtaatgcg ctggcggtgc tggtgattgc caagtgggaa cacaaatttg
    12541 accgcaaaaa ggcgctggct tatgagcgtg aagtgctggg caaatttgat aaaactgcgg
    12601 atcaataatt gaagattgcc ggggttatcc acccggcaat gtgtggatgc ctgatgcgac
    12661 gctggtgcgt cttatcaggc ctaccttaac cgttggttaa tttttcaaac tcttcacacc
    12721 cggtatcaaa cccttccata cagctcaggt tcaaccagtg cagcgccttt tgtttgttct
    12781 tctcgataaa accttcctca ccgtttaaga acatcattcc cgcccagtac tcggaataac
    12841 cggttcggga aattgcagag ctgcgtttga aataccaggt tgccttgtca tcgtcggcct
    12901 taatgcccac gccgttggcg taaatcaaac caagcagcat ttgggcatcg acggcggagt
    12961 cattctccag atcttccgag gcgttttcta acagcgaaat cgcctttgga taatccggtt
    13021 tacccgcttg agtatttacc agaatatgcg ccagcgttac ttcaccttct ttactgcccg
    13081 cttgcgccgc tttctctgca agaacttttg cttgtggata gtccagactg accggattgg
    13141 tgattttgat ctgcgccagc agcgcgcagg catcagcatc accgctgtcg gcggctttct
    13201 gtgcccaata ttcggctttg ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat
    13261 attgtgcgcg tcggtccccg gcctctgctg cctttaaata ctggctgcca ggctcggagt
    13321 cgttcgcgtg ggcaaagaat gtcaaaaaca acatcaatgc gataattttt ttcattttga
    13381 taatacgatt gggtagggag taaccagtat aaagagttcg aatggataaa aaaacagcct
    13441 ccgaaaggag gctgtagaaa ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt
    13501 tcaacttgtt gtactcgtca atcacgtact gctcggcggc gcgctgatcg gcgatcggct
    13561 cgacgcgcac ggcgcagtat ttgtattccg gcgttttcgt aatcgggctt aagttttcgg
    13621 taaccagctc gttacaggca ccaatccacc actggtaggt catgtaaatc gcccctttgt
    13681 tcggacgatc gctgacctgc gcacgggtga taattttgcc tttacgcgag tgcacccaaa
    13741 ccaatgcctc atcttcaata cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc
    13801 caggttcatc agccagcgcc gccagtgccg cacagttacc ggtcatcgaa cggcaagagt
    13861 agtgaccaac ttcacgcacc gttgacagta ccatcgggta ctcgtcggtg agtttgtcga
    13921 ttggcgctac ccagtcgcag gtgaagaact gtgccagacc gttcggggta tcaaacttct
    13981 ctttaaacag ataagaagtc ccctgatcgg catctgaagt attgcggcaa ggccactgaa
    14041 tgaagcccag ttcgcccatt ttctcgtaag tcgcaccgta gaaatccggg cacagatgac
    14101 gcaactcatc ccagatctcc tgggtgttgt tgtagtgcat cggataaccc atacgggtgg
    14161 cgatttcact gatgatttgc cagtccgttt tcagatccca tttcggttca actgctttga
    14221 agaaacgctg gaagccacgg tcagccgcag taaacacgcc ttcatgctcg ccccacgacg
    14281 ttgacggcaa aatgacatcc gccgccgacg cggttttggt cataaagatg tcctgaacga
    14341 taaccagttc cagatcttca aaggctttac gtactgccga cagctccgcg tcagtttgca
    14401 gcggatcttc gcccataatg tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca
    14461 gctcgctgat gcgataaccg gtatgagcag gcaggctttc cacgccccag gctttggcga
    14521 atttctcgcg gttagccgga tctttcacgt actggtatcc cggatacgta tccggcagcg
    14581 cgcccatatc gcaggcaccc tgcacgttgt tctgaccacg aaccgggttt acacccgcat
    14641 gcggcttacc gaggttaccg gtcagcatcg cgaggctggt cagagaacgc acggtttcca
    14701 cgccctggta gaactgggtt acacccatgc cccacaggat ggcggcgctt ttcgcctggg
    14761 catacatccg tgccgcctga cgaatctcac tggcgctgac gccggtgata tcttcaaccg
    14821 actccggcgt gtagccttca acgattttac gatactcttc aaagccttct gtacgggaag
    14881 cgacgaacgc tttgtcgtac agattttctt caataatgac atggcccatc gcattcaaca
    14941 gcgcgatgtt cgagccgttt ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa
    15001 ttttgcgcgg atcgcagacg ataattttcg ccccgttacg tttagcgtta attacgtgat
    15061 tcgccacgat tgggtgggaa tccgccgggt tgtacccgaa aacgaacact aaatcggtat
    15121 tatcaatttc gttaatagca ttgctcattg cgccattacc gaccgattgg tgcagacctg
    15181 caaccgatgg gccgtgtcag acacgagcgc agcagtcaac gttattggta ccaataacgg
    15241 cgcgcgcaaa tttttgcatt acatagttgg tttcgttacc cgtaccacgc gaggagccgg
    15301 tcgtctggat ggcatccgga ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt
    15361 aattcagtgc ctcatcccag gaaacaggtt cgagtttgcc gccacgctgg cgacggatca
    15421 tgggggtttt caggcgcggg gtcaggatct gggtatcgtt aatgaaatcc cagccataat
    15481 aacccttcag acacagggta ccctggttgg ttttcccctg cgctgcctcc gcccggacga
    15541 ttttgccgtt atcgacgacc aggttgattt tgcaacctga tgcgcaatag gggcaaaccg
    15601 tgacgacttt tttcatcggt ctcgctccag ttaatcaaat cacgcatacg cgctctcgac
    15661 tacagtatgc atcttttatg ccacatttta tttggggtca ttccctgata ttacgggcac
    15721 tatttattca aaactctgac gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa
    15781 ataccgcgtg acagccatca cgcggcagac attttatttt ttctcgacga cgggagccgc
    15841 ctgatacccg ccgcccagcg atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc
    15901 cagcatcagt aatgacatct cttcggcaag tactggcaac cgggcttcag tggcctgtaa
    15961 gcggctggtt aagccgcgct gataggcggc ctcggcagcg cgctgggtga agcgcgttgc
    16021 ttccacgcgt tcagcctgca tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt
    16081 gacggcaacg tcacgcaccg cgttcagtac tgactggttg taacgttcaa tcatcatgtt
    16141 gctggcggcg cgcgtgcctt cgagattggc attcaaccgt ccaccgtcaa acagcggcaa
    16201 tttcagaccc gggatgaagt tgaactggcg actggttttt ttgaataagg tatccaggtg
    16261 gatggcatca aggccgaaaa acgctttgat atcaaagctc gggtagaaca gcgcccgcgc
    16321 ggaatccacc tgatctaatg atgcctgaac ataccagcgc atggcttgca gatccgggcg
    16381 tctggcgagc aactcataag agagtgtcgc cggaatgccg gtctggactc gtggtaatgc
    16441 caccggtttg atctccggca tatcgctggc ccccgcgcca atcaatgcac gcagagattc
    16501 tcgcgtttcg gtgatttgcc ctttgacggc agcaatttgt ttatcgaccg ccagaatctg
    16561 tgcccgcgcg ccgtggaaag gcacttgtgc ttccagaccg tgcgccactt tactctggtg
    16621 cgctttcacc gcgtaatcaa tcacatcgcg agtttgttct aacagatcga gcatctgata
    16681 gctggcctgc atactgtaat aaagctgcgc tacgcccgtg gtcagcgata gctctactgc
    16741 tgcggtttct gccagcgcgg cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg
    16801 cacaccccac aaatcaagat ccagtccggc aaacaaacct actgtggctt ccgtatagta
    16861 cggcccgtcc atacccagtg cgggcgcatc catcgcataa gggcttaaaa agccgttcgc
    16921 cgagacgcgt tggcggttga gcatccctaa cgctgcgacc tgtaattgtg aaccggcatc
    16981 taacaaatcg gcctgggact gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg
    17041 tgaaccactt agcgtccgtt ggatcagcgc atccagctgc gggtcattga gttgtttcca
    17101 ccactgcgcc tgcggccagc cggagctggc aagatgaata tcgtcggcca gtttgatttg
    17161 ttccggtttg agctgttgat gaggtgcaga atccttacgt accagggcac agccggaaat
    17221 cagcgtcgtg ctgccgagaa tgctgcacaa cagcagacgt gaaagttgac gattgatcat
    17281 tgcgtggctc cttgtgcctg ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc
    17341 aagcgggcga aaagctggca gacgtgttgc tcctgtttta ataaggtggg caggatggcc
    17401 tgcgaggcgg gtgtctcttc aagcgttatt tgaggagaac ggctgagtgc ggttgccaga
    17461 ccggcagcgt atttctccag ggcgtcggca aactgagctg ctctgtccgg ctgtagtgcg
    17521 ttatccagcg cctgcgccga gttccaggtg gcatcccagg cgtgaagaat atcgcggccc
    17581 tgacgaataa ccgtttgcga acgttcaatc agtaaggcgc gttcttcgct gtccagttga
    17641 cgctccagcg ccacgcgttg gcacatctct tcacaggcat taaacgccgc atgcagaccg
    17701 atacgaattt gcagataagt gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt
    17761 actttactta acatacccag cgtgccagcc agtttttgcg gcagtgtgcg cgcttcactt
    17821 tcaggccaga caaaggtgta aatcaccgcg gacaccacgg taccaatgat gatacccatg
    17881 gcgtgatcgc gaatttccac caggtcgtac accgggccaa aaacgttttc gagcgtggcg
    17941 agcgcgaagg tgaccaccat ctgtgtgccg atataagaag agcgttcaga gctggtggca
    18001 atccatgcgc ccaacaggaa aatcggtgcc agcacaaaca gcaattcgac aatattgtcc
    18061 agccagggca tgaccagtag cgtgaataac agcgccagaa tcgcgccgca aaaggccccg
    18121 ccaaaacgca gcaccatctt ctggtacgac gaaccgacat ttgggttagc gacgatcacg
    18181 catgtcagca tacaggtgtg aatgccttcc cagtccacgc cgctgtaaaa ggtgtaacag
    18241 atcaaacagg cgagcagcgt ttttaccgcg tagcgcatat agtctggatt ggtaaaagca
    18301 tcggcggcca ttgatggcgg tttggctgcg ggcgttggcg gcgtattcgg gtccatctga
    18361 cccagttgta acaacgtctg gcagatattc tccaggttac attcccgtgc cgccatcgct
    18421 tcactttcac tgatccgcca gtcgctttgc cagcactgac cttcagcaac ggcatgctgc
    18481 agcttattga tttctgaggc taatttttgc cgaaattcaa taattgcctg agaatcagca
    18541 aaagaggtgg gatcgtagcg attcagcgtc gagtaaatgt aggttaccgt tgccacgcag
    18601 ctttgccacc atgcgctttg agttcgccag ttggcatcgt ccgcgagaca aaagacattc
    18661 agtttttgca gcgccagcgc ctctctttca atccgcgttt cgggtagcgg tgcgaggctg
    18721 tccgtcaggt ggctaatggc atcatcaagc cgatcattaa gcgcctgatg catttgcgaa
    18781 atggcacgac tgggaaacca cagcacgccg attaacgtca tcagcaaggt tggatagagg
    18841 ccaacaacga tacaccatag cgttaagcgc acgaccactt ccggatagtc gagcatggcg
    18901 gggaaggttt gcccgtaaat ggcgacaatg gcgacggcga aaaagaccag tcccagccga
    18961 tgggtgcgca tcaaaaacat acagcccatc aggatcggcc cggcgatgat caatcggatc
    19021 aacggttcgc cgtatgacca tttgtagatc aaaaacaggc tgccgatctc cagcatcgtg
    19081 gcgaccacaa ataagatcgc gacaaatttg gtgtaaaacg cgttcgactg aataccgtaa
    19141 aacagcactg ccagcgataa cgccacgaaa gggatctcaa aggtcatcga gatcagaatc
    19201 accagcaggc agcctaccca gagttgcacg gtctgcggca cgcggcctgg tcgccgctcg
    19261 cttaactctt catgaaagaa cgccagcagc ctgaccaccg gtaatggcag ggagttgagc
    19321 gcgctcatta ttgcggctca agattagcga ctgccgaagc gccgatgcgg aacatttccg
    19381 ggtcaggttt atcgaccatg attttgaccg gaaaacgctg ggcaacgcgg acccagttaa
    19441 tagagcgaga aactttcggc aggccgccca gcaccaggcc gccgtcatcc ggtagcacgc
    19501 cgtagccaat cgaatccact ttaccctcga aggttttgcc gctgtcactc atcaggcgaa
    19561 tcgttgcggg tgtacctgag cgaatatttt tcagatcggt ttcgcggaag ttggcgatca
    19621 cgtaccagtg acgagtatcg attagggtaa aaatagggcg catcgtagat gcaaactgcc
    19681 cgacggaggt tttgagggaa atgacccggc catcaaacgg cgcgcgaacg gtcgccattt
    19741 ccagatgcag tttggtcagg gcaatatccg cttcgaccgc cgcacgctgg gcaactaatg
    19801 catccacgcc gctgacggcg ctggcggctg actgcgcctg caacaataca gcattaagat
    19861 cggcttctgc agcgcgctgc gcggtcctcg cacggtcaac gtcttccgct gagacaaaac
    19921 cttctttcag taatggctcg gtgcggcgta atgtatctgt ggcctgtttc gcggcggcac
    19981 gggctttttc taccgtggcg ttaaccgagt cggcaccaaa ctgttgcgcg tcaacgctac
    20041 gctgggtgag cataatttgc ttatccagcg ccgcgaggga ggcttcagct ttcgccagat
    20101 tggcttcgta cgggcgcggg tcaatgcgga acagcaaatc gccctgtttg actgcctggt
    20161 tgtcggtgac cgccagttct acaatgcgac cgctgacttc cggcaccaca tcaatggtat
    20221 ctgctgacgc gtaagcgtca ttagttgatg gcgcactgtc tacgcgccag ataacgaaaa
    20281 caagggcaac cagcgccaac gcaaccacta acagagcagg gaatttactg cgaggagctt
    20341 ttttcggcgt actttccatg agcgcgtctc aaatagatta gaaaaatgcc agccagaaca
    20401 gcatggcgta gagagcaaaa agggcggtat aaataattcc gacaaatgcc agattgatat
    20461 ttgcccgctg aataactcgc ctcgtgatga gcgtcaaaat aaggctggca atggcgcaga
    20521 aaaaccagct gggataataa gcgccgatca ccgggattgc cggagataat gagcatcccg
    20581 aaaccatgaa gacgggaatt atccaggctg tttttttgag ttgcatcgcc atgcgagaga
    20641 gaactgttgg catattaaat acatcctgtc tgctgcgaac aatatctgta gtgaatatca
    20701 acatccattg aaggcgtcag tatggctatt ttcttgatga ataaaaatag ctttattcag
    20761 ccaccgcatt cataacttat
//
LOCUS       DAZKIV010000078        20698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZKIV010000078 DAZKIV010000000
VERSION     DAZKIV010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.078"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            198..1580
                     /gene="dosC"
                     /locus_tag="ACHOT6_002778"
     CDS             198..1580
                     /gene="dosC"
                     /locus_tag="ACHOT6_002778"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFP9947966.1"
                     /translation="MEMYFKRMKDECTGLVEQADPPIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            1605..3995
                     /gene="dosP"
                     /locus_tag="ACHOT6_002779"
                     /gene_synonym="pdeO"
     CDS             1605..3995
                     /gene="dosP"
                     /locus_tag="ACHOT6_002779"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFP9947967.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTTRDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLIDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLESNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYLLST
                     AHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIFAET
                     GELYGIEALARWHDPLHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRSQNI
                     HIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFKRIQ
                     ILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAITSI
                     GQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLKI"
     gene            4253..4834
                     /gene="ddpX"
                     /locus_tag="ACHOT6_002780"
     CDS             4253..4834
                     /gene="ddpX"
                     /locus_tag="ACHOT6_002780"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFP9947968.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            4848..6398
                     /locus_tag="ACHOT6_002781"
     CDS             4848..6398
                     /locus_tag="ACHOT6_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFP9947969.1"
                     /translation="MKRSISFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            6400..7422
                     /locus_tag="ACHOT6_002782"
     CDS             6400..7422
                     /locus_tag="ACHOT6_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9947970.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            7419..8315
                     /gene="ddpC"
                     /locus_tag="ACHOT6_002783"
     CDS             7419..8315
                     /gene="ddpC"
                     /locus_tag="ACHOT6_002783"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFP9947971.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            8312..9298
                     /locus_tag="ACHOT6_002784"
     CDS             8312..9298
                     /locus_tag="ACHOT6_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9947972.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTLRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            9291..10217
                     /locus_tag="ACHOT6_002785"
     CDS             9291..10217
                     /locus_tag="ACHOT6_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFP9947973.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(10273..10704)
                     /gene="osmC"
                     /locus_tag="ACHOT6_002786"
     CDS             complement(10273..10704)
                     /gene="osmC"
                     /locus_tag="ACHOT6_002786"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFP9947974.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            11049..11264
                     /gene="bdm"
                     /locus_tag="ACHOT6_002787"
     CDS             11049..11264
                     /gene="bdm"
                     /locus_tag="ACHOT6_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFP9947975.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEQGVVTENDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            11366..11503
                     /gene="sra"
                     /locus_tag="ACHOT6_002788"
     CDS             11366..11503
                     /gene="sra"
                     /locus_tag="ACHOT6_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFP9947976.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            11660..13357
                     /gene="maeA"
                     /locus_tag="ACHOT6_002789"
     CDS             11660..13357
                     /gene="maeA"
                     /locus_tag="ACHOT6_002789"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9947977.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            13491..14501
                     /gene="adhP"
                     /locus_tag="ACHOT6_002790"
     CDS             13491..14501
                     /gene="adhP"
                     /locus_tag="ACHOT6_002790"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFP9947978.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMVCCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHSAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRDRMVIDF
                     RH"
     gene            14647..14931
                     /locus_tag="ACHOT6_002791"
     CDS             14647..14931
                     /locus_tag="ACHOT6_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFP9947979.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(14982..15635)
                     /gene="fdnI"
                     /locus_tag="ACHOT6_002792"
     CDS             complement(14982..15635)
                     /gene="fdnI"
                     /locus_tag="ACHOT6_002792"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFP9947980.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(15628..16512)
                     /gene="fdnH"
                     /locus_tag="ACHOT6_002793"
     CDS             complement(15628..16512)
                     /gene="fdnH"
                     /locus_tag="ACHOT6_002793"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFP9947981.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(16525..19572)
                     /gene="fdnG"
                     /locus_tag="ACHOT6_002794"
     CDS             complement(16525..19572)
                     /gene="fdnG"
                     /locus_tag="ACHOT6_002794"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(18985..18987),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9947982.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENDKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVESLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            19804..20685
                     /gene="yddG"
                     /locus_tag="ACHOT6_002795"
     CDS             19804..20685
                     /gene="yddG"
                     /locus_tag="ACHOT6_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFP9947983.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGICWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5051 a         5267 c         5283 g         5097 t
ORIGIN      
        1 gatattcgct gtcaccgaaa aataattacg atgggctata ctcttctgaa gaacagtagc
       61 gtagattgaa actgtcctaa gtcgcttatt tcttttcagt atatcttcat atttcaggag
      121 aatatggtgc agttagtcga tctcgcacgt tgcgttttga ttccgtctga tttcccagcc
      181 ttataagggt gtgaattatg gagatgtatt ttaaaagaat gaaagatgag tgtaccggac
      241 ttgtcgaaca ggcagatccg cccattcgtg ctaaagccgc ggaaattgcc gttgcgcatg
      301 ctcattatct gagtattgag ttttatcgaa ttgtccgcat cgacccgcat gccgaagaat
      361 tcttgagtaa tgaacaagtt gagcggcagt tgaaaagtgc gatggaacgc tggattatta
      421 acgtgctttc tgcccaggtt gacgatgtcg aaaggctaat acaaatccag cataccgtcg
      481 cggaagtgca tgcccgcata ggaattccgg tagaaattgt cgagatgggg tttcgggtgc
      541 tgaaaaaaat cctctatccg gtaatcttct cttcggatta ttccgccgca gaaaaacttc
      601 aggtctacca tttttcgatt aacagtattg atatcgcgat ggaagtgatg acccgcgcgt
      661 ttacctttag tgacagtagt gcctcaaagg aagatgaaaa ctatcgtatc ttctcgttac
      721 tggaaaacgc cgaagaagaa aaagaacggc aaatagcctc aatactttca tgggaaatag
      781 atattatcta taaaatcctg ctggattctg atttaggcag tagtttgcct ttaagccagg
      841 ctgattttgg cctgtggttt aaccataaag gtcgacatta ttttagtggc attgccgaag
      901 taggccatat ctcccgtctg attcaggatt tcgacggtat tttcaatcaa accatgcgta
      961 acaccaggaa tttgaataac agaagtctgc gggtgaaatt tttattacag ataagaaata
     1021 ccgtatcgca aattattacc ttgctgcgtg aattgtttga agaagtatcg cgccacgaag
     1081 tcggtatgga tgtactgacg aaattactta accgccgttt cctgccgact atcttcaaac
     1141 gcgaaattgc ccatgccaac cggaccggta caccgctgtc agtgctgatt attgacgttg
     1201 ataaattcaa agagatcaac gatacgtggg gccataacac tggcgatgaa attctgcgta
     1261 aagtctctca ggccttttat gacaacgtcc gcagtagtga ttatgttttc cgctacgggg
     1321 gcgatgaatt tatcattgtt ttgactgaag cttctgaaaa cgaaacgtta cgtaccgcag
     1381 aacgtattcg cagtcgggtg gagaaaacca aactgaaagc cgcaaacggc gaggatattg
     1441 ccctctcact ttccatcggt gccgccatgt ttaatggtca tcctgactat gagcgcctca
     1501 ttcaaatagc cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac cgtgttgaac
     1561 tctggaaagc cagtctttag atgcgccagg atgcagaggt aatcatgaag ctaaccgatg
     1621 cggataatgc cgccgatggc atttttttcc ccgcccttga gcaaaatatg atgggtgcgg
     1681 tgttaattaa cgaaaatgat gaagtgatgt ttttcaaccc cgccgcagag aagctatggg
     1741 ggtacaaacg tgaagaagtc attggcaata acattgatat gctgattccg cgagatttgc
     1801 gtcctgcgca tcctgaatac attcgtcata accgtgaagg cggtaaagcg cgcgtcgagg
     1861 ggatgagtcg ggagctgcag ctggagaaaa aagacggcag taaaatctgg acccgttttg
     1921 cgctatcgaa agtgagcgcc gaggggaaag tttattacct ggcgctggta cgggatgcca
     1981 gcgtagaaat ggcgcaaaaa gaacagaccc gacaattgat tattgccgtt gaccatctcg
     2041 accgaccggt gattgtcctc gatccggaac gccatattgt gcagtgcaat cgcgcattta
     2101 ccgaaatgtt tggttactgc attagcgaag ccagcggtat gcagcccgat acactcctga
     2161 acattcctga attccctgcc gataaccgca ttcgtttaca acagttgcta tggaaaacca
     2221 cccgcgatca ggacgaattt ctgctgttga cgcgcaccgg tgaaaaaatc tggattaaag
     2281 cttctatcag cccggtttat gacgtgctcg cgcatctgca gaacctggta atgaccttct
     2341 cggatatcac cgaagaacgg caaatccgcc agcttgaagg caatattctc gccgccatgt
     2401 gcagcagccc gccatttcat gaaatggggg aaatcatttg tcgtaacatc gaatctgttc
     2461 tcaacgaatc gcatgtttcg ctgttcgcac tgcgcaacgg gatgccgata cactgggcgt
     2521 catcttcgca cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc attcgtcagc
     2581 gtgatggtgc gcctgcgggg atcctgcaaa ttaaaacctc gtcaggagca gaaaccagcg
     2641 cctttatcga acgcgtggca gatatcagcc agcatatggc cgcgctggcg ctggaacagg
     2701 aaaaaagccg tcagcatatt gaacaactca tccaatttga tccgatgacc ggtctgccaa
     2761 atcgcaataa cctgcacaat tacctcgatg accttatcga caaagccgtc tctccggtgg
     2821 tgtatctcat cggtgttgac catattcagg atgtgattga tagccttggc tatgcgtggg
     2881 ccgatcaggc attgctggaa gtggtcaatc gctttcgtga aaaactcaaa ccggatcagt
     2941 atctctgtcg tatcgaaggt acgcagtttg tcctcgtgag cctggaaagt aacattaccc
     3001 aaatcgccga tgagctacgg aatgtggtca gcaagccgat aatgattgac gataaaccct
     3061 tcccgcttac cttgagtatt ggcatcagct acgacgtggg taaaaaccgc gattacttgc
     3121 tctccactgc tcacaatgca atggattata ttcgcaagaa tggcggtaac ggctggcagt
     3181 tcttcagccc ggcgatgaac gaaatggtaa aagagcgttt ggttttaggc gcagcgctga
     3241 aagaagcgat tagcaataac caactgaaac tggtttacca gccgcaaatc ttcgcagaaa
     3301 cgggtgaact gtacggcatc gaagcccttg ctcgctggca cgatcccctg catggtcatg
     3361 tgcccccttc acggtttatt cctctcgcag aagagattgg tgaaatcgaa aatattgggc
     3421 gctgggtcat cgcggaagct tgccgtcagt tagcagaatg gcgtagccag aatattcata
     3481 tcccggcgtt atccgtgaac ttgtcggcgc tgcactttcg cagtaatcaa ctgcctaatc
     3541 aggtgtctga tgcaatgcac gcctggggta ttgacggcca ccagctgacg gtagaaatca
     3601 cggaaagcat gatgatggaa cacgataccg aaatctttaa gcgcattcag atcctgcgtg
     3661 atatgggcgt gggcttatcg gtagatgatt ttggtacggg cttttccgga ttatcccgct
     3721 tagtcagtct tccggtaacg gaaatcaaaa ttgacaaaag ttttgtcgat cgttgtctga
     3781 ccgaaaaacg catccttgcc ttacttgaag ccattaccag cattgggcaa agcctcaatt
     3841 taaccgtcgt ggcggaaggc gtcgaaacca aagagcaatt tgagatgcta cgcaagatcc
     3901 actgtcgcgt tattcaggga tatttctttt cccgccccct acccgccgaa gaaattccag
     3961 gctggatgag cagcgtgtta ccgctgaaaa tctgacaaat tcctctcgcc cgcactcgcg
     4021 ggttttcatt taacgtgaca ctgtcactta aatactgtga tttcagctac gattccggga
     4081 gattccttcc ttaacttccc ctcttgcacc aaaactgcac tacgctgcac aaatgttgat
     4141 caaaagttaa aaaatataac tttaagccag cattctggcg cttatcccgg catggcatga
     4201 gatctgcata agcggaaagc gcagcaattt ttgtcttata caggaaccgt ttatgtcgga
     4261 taccaccgaa ctggttgatt tagccgtaat cttccctgat ctggagatcg aattgaaata
     4321 cgcctgcgct gataacatca caggtaaagc tatttatcag caagcgcgtt gtctgttaca
     4381 caaggatgcg attaccgcgc tggcgaaaag tatcagcatc gcccagctgt caggcttaca
     4441 actggtgatt tacgatgcgt atcgcccaca acaagcacag gcgatgttgt ggcaagcctg
     4501 cccagacccg caatatgttg ttgatgtgac ggtcggttct aatcacagcc gtggcacggc
     4561 gatcgacctg acgcttcgtg atgagcacgg gaacatcctc gatatgggcg cagggttcga
     4621 tgaaatgcac gagcgctccc atgcctatca cccttccgtc ccgcccgccg ctcagcgcaa
     4681 tcggctgttg ctgaatgcga taatgactgg tggcggtttt gtcggtatct ccagcgaatg
     4741 gtggcacttc gaattacccc aggcagcgag ttaccctctg ctcgctgatc aattctcttg
     4801 ttttatatcc cctggcacac agcacgtcag ttaatcagga aactgtcatg aagagatcga
     4861 tatcgtttcg tcccacattg ctcgcgctcg tccttgccac aaatttcccg gttgcgcacg
     4921 ccgccgtacc gaaagatatg ctggtgattg gtaaggccgc cgatccacaa accctcgacc
     4981 cggcggtaac aatagataat aacgactgga cagtgaccta cccgtcttat cagcggctgg
     5041 ttcagtacaa aacggacggt gataaaggct caaccgacgt tgaaggcgat ctggcaagta
     5101 gctggaaagc gtctgacgat caaaaagagt ggacgttcac cctgaaagat aatgctaaat
     5161 ttgccgatgg cacacctgtc actgccgaag cagtaaaact ttcttttgag cggctactaa
     5221 aaatcggcca ggggccagca gaagcatttc ccaaagattt aaagattgat gctcccgacg
     5281 aacatacggt gaagtttacc cttagccaac cattcgcacc gttcctctac acgctggcga
     5341 atgacggtgc atccattatc aatccggcgg tcttaaagga acatgcagcg gatgatgctc
     5401 gcggcttcct cgcgcaaaat accgccggtt ccggaccatt tatgctgaaa agctggcaaa
     5461 aaggtcagca attagttctg gtgccaaatc cgcattaccc aggcaataaa ccgaacttca
     5521 aacgggtatc ggtaaaaatt attggtgaaa gtgcctcccg tcgcctgcag ctctcccgtg
     5581 gcgacattga cattgccgat gcgctgccgg tggatcaact caacgccctg aagcaggaaa
     5641 ataaagtcaa tgtggcagag tatccgtcac tgcgcgttac ctatctgtat ctgaataaca
     5701 gcaaagcgcc tcttaatcag gcggatctgc gtcgggccat ttcctggtct accgattatc
     5761 agggcatggt taacggcatt ctgagtggta acggaaaaca gatgcgcggc ccgattccgg
     5821 aaggcatgtg gggctacgat gcgacggcaa tgcaatacaa ccatgacgaa acgaaagcca
     5881 aagccgaatg ggataaagtg acgagcaaac ccaccagcct gacgtttctc tactccgata
     5941 acgatccgaa ctgggagcct attgctctgg cgacacaatc cagtctcaac aagctgggta
     6001 tcaatgtgaa gctggaaaag ctggcgaacg ccaccatgcg cgacagagtg ggtaaaggtg
     6061 attacgacat tgcgattggc aactggagtc cggattttgc cgacccgtat atgtttatga
     6121 attactggtt tgagtcagac aaaaaaggtc tgccgggtaa ccgctcgttc tatgaaaaca
     6181 gtgaggtcga taagttactg cgcaatgcgc ttgcgaccac cgaccagacg cagcgtaccc
     6241 gggactacca gcaggcacag aaaatcgtca ttgatgacgc tgcttatgtg tacctgttcc
     6301 agaaaaacta ccaactggcg atgaacaaag aggtgaaagg ctttgtgttc aatcccatgc
     6361 tggaacaggt cttcaatatc aataccatga gtaaataacg tgaccttctg gagtatttta
     6421 cgccaacgct gctgggggct ggtgctcgtg gtggcgggcg tctgcgtgat tacgtttatt
     6481 atctcgcatc tgatccctgg tgatccggcg cggttactgg cgggtgaccg cgccagcgat
     6541 gctatcgtgg aaaatattcg ccagcaactg ggactggacc agccactgta tgtacagttt
     6601 taccgctacg tcagcgacct gtttcatggt gacctgggaa catccattcg taccgggcgt
     6661 ccggtgctgg aagagttgcg tatatttttc ccggcgacgc tggaactggc tttttgtgcc
     6721 ctgctgctgg cactcctgat tggcatcccg ctgggcatac tctctgcagt ctggcgaaat
     6781 cgctggctgg atcatctggt gcgaataatg gccattaccg gaatctccac acctgcgttc
     6841 tggcttggac tgggcgtcat tgtgctgttt tatggtcatc tgcaaattct tcccggcggc
     6901 ggaaggcttg atgactggct ggatccacca acgcacgtta ccggctttta tctgctcgat
     6961 gcgctgcttg aaggcaacgg tgaagtcttc ttcaatgcgt tgcaacatct catcttaccg
     7021 gcattaacgc tggcgttcgt tcacctggga attgtcgctc gccagatccg ctcagcgatg
     7081 ctggaacaat tgagtgaaga ctacattcgt accgcccggg ccagcggctt gcccggctgg
     7141 tatatcgttt tatgttatgc gctacccaat gcgttgatcc catcgattac cgtattgggt
     7201 ttggcgctgg gcgatttgtt gtatggcgca gtgctcaccg aaaccgtttt tgcctggccc
     7261 ggaatgggtg catgggtagt aacatcaata caggcgctcg acttcccggc agtgatgggc
     7321 tttgccgtcg tggtttcatt tgcttatgtg ctggtcaacc tggtggtgga tttgctctat
     7381 ttgtggattg atccgcgtat cggacgtgga ggtggtgaat gatgctaagc gaggaaacgt
     7441 ccgccgtacg cccacaaaaa caaacgcgat ttaacggtgc aaaactggta tggatgctga
     7501 aaggcagtcc gctcaccgtg accggcgcag tcatcattgt attaatgcta ttgatgatga
     7561 ttttttcacc gtggcttgcg acgcatgatc ccaacgccat tgatttaacc gcccgccttt
     7621 tgccgccttc tgcggcgcac tggtttggca ccgatgaagt gggacgcgat ctgttcagcc
     7681 gcgtactggt cggcagtcag caatcaattc tcgccggatt agtcgtggtt gccattgcgg
     7741 gtatgattgg ttcgctactc ggatgtctat ccggtgtgct tggcggacgc gcagacgcca
     7801 tcatcatgcg catcatggac attatgctgt cgattccttc gttggtactg acaatggcac
     7861 tggctgccgc tctcgggccg agtttattta acgccatgct ggcgattgct attgtgcgaa
     7921 ttccctttta tgtgcgcctg gcgcgggggc aagcattagt tgtacgccag tatacctatg
     7981 ttcaggcggc gaaaaccttt ggtgcgtcgc gttggcatct gatcagctgg catattttac
     8041 gtaactccct gccgccgctg atcgtgcagg catcgctgga tatcggtagc gcgattttaa
     8101 tggccgccac gttgggattt attggcctgg gtgctcaaca accgagtgct gaatggggcg
     8161 cgatggtagc aaatggtcgc aactatgtac tcgatcagtg gtggtattgc gcatttccgg
     8221 gggcagcgat tttgcttacc gccgttgggt ttaatctctt tggtgatggt attcgcgatc
     8281 tgcttgaccc gaaagcagga ggaaagcagt catgacccaa cccgttctgg acattcaaca
     8341 gctgcatttg agtttccccg gttttaacgg tgatgttcac gcgctcaaca atgtgtcctt
     8401 gcagattaac cgcggtgaaa ttgtcggtct ggtgggagaa tccggctcag gtaaatcagt
     8461 caccgcaatg ctgattatgc gtctgctacc gacgggcagt tattgcgtac atcgggggca
     8521 gatttcactg ctgggagacg atgttcttaa cgctcgggaa aaacagcttc gtcaatggcg
     8581 cggcgcacga gtggcgatga tctttcagga accgatgacc gccctcaatc cgacacttcg
     8641 aataggtctt cagatgatgg acgtgatccg ccatcatcaa ccaataagtc gtcgggaagc
     8701 cagagctaaa gcgattgccc tgctggaaga gatgcaaatc ccggatgccg tggaagttat
     8761 gtcgcgctat ccgtttgagc tttcaggtgg tatgcgccag cgggtaatga ttgcgctggc
     8821 attctcctgc gagccgcaat tgattattgc cgacgaaccg actacggcgc tggacgtcac
     8881 ggtgcagttg caggtactgc gtttgcttaa acataaagcc cgcgccagtg gaactgcggt
     8941 tctgttcatc agccatgata tggccgtggt gtcgcaactg tgcgatagcg tttacgtgat
     9001 gtatgccgga agcgtaattg aaagcggcgt gacggcagat gttatccatc atccccggca
     9061 tccgtatacc attggtttgc tccaatgcgc accagaacat ggagtaccac gccagctatt
     9121 acccgccatt ccagggacgg taccaaacct cacccatttg cctgaaggct gcgcttttcg
     9181 cgatcgttgc tatgcggcag gtgcacagtg tgaaaacgtc ccggcgctga cagcgtgtgg
     9241 tgacaacaac cagcgctgcg cctgttggta tccgcagcag gaggtcataa gtgtctgaca
     9301 cgttattaac gttacgcgac gtccatatca atttcccggc ccgtaaaaac tggcttggta
     9361 aaactacgga acatgttcat gccattaatg gtattgattt acagatccgc cgtggtgaaa
     9421 ccttagggat cgtcggcgag tcaggctgcg gcaaaagcac cctcgcacag cttttaatgg
     9481 gcatgctgca accgagccac gggcagtaca tccgttctgg ctcacaacgc attatgcaga
     9541 tggtgtttca ggacccgctc tcttcgctca atccgcgctt accggtgtgg cgcatcatca
     9601 cagaaccgct ctggatagct aagcgtagta gtgaacaaca gcggcgagcg ttggcagagg
     9661 agctggctgt gcaggtgggt attcgtccgg agtatctcga ccgcctgcct catgcgttct
     9721 ccggcgggca gcggcaacgt atcgccattg ccagagcact ctcttcgcag cctgacgtga
     9781 ttgtgcttga tgagccaacc tctgcgctgg atatctccgt gcaggcgcag atcctcaatt
     9841 tactggtaac gctacaggaa aatcacgggc tgacctatgt gctgatttca cacaatgtct
     9901 cggtgatacg tcatatgagc gatcgggtgg cggtgatgta tctcgggcag attgtagaac
     9961 tgggcgacgc gcaacaggtg ctaacggctc cggcacatcc atacacccga ttattgctgg
    10021 attccctccc cgccattgat aaaccgctgg aggaagaatg ggcattacgt aaaacggatc
    10081 tgccgggaaa ccgcacgttg ccgcaaggct gttttttcta cgaacgttgc ccccttgcaa
    10141 ccagcggatg tgaagtccgg caatcattag caataaggga ggacggacgt gagctccggt
    10201 gctggcgggc gctgtagaga tgctaacaac gcatcaggca ttcacgcgcc tgacgcgtca
    10261 tccggcaatg ctttacgatt tcaactggta atccagcgta atttccgctt tcagtacctg
    10321 agagaccggg catcctgctt ttgctttctg gatgatgccg tcaaaggtag aggcatcaat
    10381 acccggcacc gcaacttcac tcttcagtgc gattttcgta attgcaaaac cggcatccac
    10441 tttatccagc gacacatcgg cggtggtatc aatcgatgtt ggcgtgaatc ccgcttcccc
    10501 cagcattaat gaaagcgcca ttgagaaaca tgcggcatgc gctgcgccaa tcagttcttc
    10561 agggttggtt cctttttcgc cttcaaaacg cgtgttaaat ccatacggct gttggttcag
    10621 cacgccactc tcggtggata ctgttccctt cccgcgttta atatcgcctt cccagtgtgc
    10681 ctgacctttc ttatggattg tcattgttgc tctcctgtgg gcttaaaaag gaaattatag
    10741 actacgtgag aaacagcacg aggataagca ggatattccg aataaaatac ggcgaaaata
    10801 ttacttctgc acttaaatta aattcccaca atagataacc gggaatatat atctcaagag
    10861 atattgcatc aatatcaatc aactcaccac aataataata catatcaata tcatggagtt
    10921 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
    10981 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
    11041 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
    11101 atgccattga gcaaggcctt cgggcagagc aaggtgttgt cactgaaaat gacattctca
    11161 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
    11221 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
    11281 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
    11341 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
    11401 actggaccat aaaatttcga accagcgcaa aatagttacc gaaggtgaca aatccagcgt
    11461 agtaaataac ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
    11521 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata
    11581 cttggggccg acgccctggc ggtaaagcaa agacgataaa agccccccag ggatggatat
    11641 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc
    11701 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat
    11761 ggaagaacgc cgtaacttca atctgctggg gttactgccg gaagtggtcg aaaccatcga
    11821 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
    11881 catctacctg cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa
    11941 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
    12001 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accggcacaa
    12061 tatggacgat attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga
    12121 cggtgaacgt attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
    12181 taaactgtcg ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt
    12241 gctggatgtc ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg
    12301 taatccgcgt atcactgacg atgaatacta tgaattcgtt gatgaattta tccaggctgt
    12361 gaaacaacgc tggccggacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat
    12421 gccgttactt aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac
    12481 tgcggcagta acagtcggca cactgatcgc agccagccgc gcggcaggtg gtcagttaag
    12541 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
    12601 catcgcccag acccagcgcg aaggattaag cgaggaagca gcgcggcaga aagtctttat
    12661 ggtcgatcgc tttggcctgc tgacggacaa gatgccgaac ctgctacctt tccagaccaa
    12721 actggtgcag aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgttgtcact
    12781 gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
    12841 gctgtttacg gaagagatca ttcgtgagat gcataaacac tgtccgcgtc cgatcgtgat
    12901 gccgctgtct aacccgacgt cacgcgtgga agccacaccg caggacatta tcgcctggac
    12961 cgaaggtaac gcgctggtcg ccactggcag cccgtttaat ccagtggtat ggaaagataa
    13021 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccgggcatcg gcctgggggt
    13081 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct
    13141 tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga
    13201 tattcagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg
    13261 cgtggcggtg aaaacgtctg ccgaagcttt gcaacaagcc attgacgata atttctggca
    13321 agccgaatac cgcgactacc gccgtacctc catctaagcc tgcacccggt agtgaaggct
    13381 accgggcaat tttcctctcc ctttttcaga actcatccat actgggtagt ggcgaataaa
    13441 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg
    13501 cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac
    13561 atggcgaagc cctgctgaaa atggtgtgtt gtggtgtatg tcataccgat cttcatgtca
    13621 agaatggcga ttttggtgac aaaactgggg taattctggg ccatgaaggc atcggtgtgg
    13681 tggcagaagt gggtccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
    13741 ggttctacga aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc
    13801 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
    13861 tcgccgatta cgcggtaaaa gtgccagatg gtctggactc ggcggcggcc agcagcatta
    13921 cctgtgcggg ggtcaccacc tacaaagccg ttaagctgtc aaaaattcgt cccgggcagt
    13981 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg
    14041 tctttaacgc caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg
    14101 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
    14161 agaaaaccgg tggcgctcac tctgcggtgg taacagcagt agctaaagct gcgtttaact
    14221 cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt
    14281 ctatgagcct ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg
    14341 tcggcacgcg ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
    14401 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag
    14461 gcaaaatccg tgaccgcatg gtgattgatt tccgtcacta agaggccttt gctgcgactg
    14521 ccatgttcgg gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt
    14581 tgcgtaggaa ggaacaataa tgatagtgtt cccagtgaag gaacgctact gagagtaaag
    14641 acactgatga aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac
    14701 gaacttaatg tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca
    14761 agccgattac tgaccggaaa agcagctttg acaccagaaa tggcgattaa actttccgtg
    14821 gtgatcggca gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag
    14881 gctgaaaaaa cggtggatgt gtcgcgactg cgccgtctgg ttacgcaata aaaaaatggc
    14941 gcttcttaaa gcgccatttt cagcttaaag ttcaaaagag attatatccc ttcttcactc
    15001 tcttttttcg cttctgcctt ctcgatttca cgataccagc gcggatggtg tttcttcgcc
    15061 cagcgacggc tcaccttccc ttcgatcatc cctttaatcg accctttcac ccagaatgcc
    15121 atatacatat ggatcaggat ggcgtggatc aggatgatac ccgcagccgc gtggatcagc
    15181 aggctgtagc gaacaacctg catcgggaag tactgcgcaa agtacggacg ccagataatc
    15241 accccggtca ccagcagcac gaaaatcatg ctcatgatcg accagaacat catcttttgc
    15301 ccggcgttgt acttaccgac atccgccact ttatgctcat tgcctttcaa tacttcgaca
    15361 atgttcaaca gccacggaat atctttctta tccgggatgt tgtgatgcac aaaacgcaca
    15421 aacataaaca tcagtgcgac gaaaatcgca atgccgaaaa acgggtgcaa aatgcgtccc
    15481 atctgcggcg taccgaaggt ttgcgtcagc cattgcagcg tcgggaagaa gaacgaaatc
    15541 ccggacagcg ccaccaggaa gaagcaaatc accacggtcc agtgacaggc gcgatcaata
    15601 aatttggtgc gcacaatcat tttcgactta ctcatgatga tcctcctcgt catcgtccac
    15661 ttccttattc gggccaatac cgatgtagtg gaaaatcaac ccggcaaaag tggcaataaa
    15721 gccagccgct gccagcggtt tcaacgcgcc tttccacagg cttaccgagg tgtcgatctt
    15781 cggatctttc ggcagaccgt gatacagttc cggctgatcg gcgtgatgca gcacgtacat
    15841 aacgtgcgta ccaccgaccc cttccgggtt gtagacgcca gcatgttcgt aaccacgcgc
    15901 tttcagtttc gccacgcgct gttccgccag ctccagcatc tccttcttgg tgccgaagtg
    15961 gatagccccg gtcggacagg ttttcacaca agccggttcc tggccgacgc tgacgcgatc
    16021 gacgcagagc gtgcatttat atacccggtt atcctctttg ttgaggcgcg gaatattaaa
    16081 cggacacccg gcaatgcagt aaccacagcc gatgcagttt tccgactgga aatcgacaat
    16141 cccgttagcg tactgaatga ttgcaccagc agacgggcac gccttcaggc agccgggatc
    16201 ttcacagtgc atacagccgt ctttacggat cagccactcc agcttgccgt tctgttcggt
    16261 ttcgctaaag cgcatcaccg tccaggactt ggcgctcaga tcggcggggt tatcgtaaac
    16321 cccgacgcag tgccccactt catcacggat gtcgttccac tccgaacacg ccacctgaca
    16381 ggctttacag ccgatacagg tggaaacgtc gataagtttt gcgacttctg ctttgtaatc
    16441 acgcacctga gaaggcggcg tgatggagtt agttgcggac cttttgataa tgtcctgcgt
    16501 ttccatagcc atctgttcgc ccccttacgc cttctcgatg ttgactaaga acgctttata
    16561 ttccggcgtt tgcgagtttg catcaccgac attcggcgtc agagtgttag cgatataacc
    16621 tttacgcgcg acaccctcaa agccccagtg gattggaata cccaccgttt caacctgctg
    16681 accatttaca ttcagcggtt tcagacgacg cgttaccaca gccaccgcgc ggataaagcc
    16741 acgcttgctt gagacagtga cacgatcgcc attattaatg cctttcgccg ccgccagcgt
    16801 ttcgctgatt tccacaaact gttccggctg agcaattgcg ttgagcaatg cgtgcttggt
    16861 ccaggtgtgg aagtgctcgg tcagacgata ggtcgtaccc acatacggga actgctcttt
    16921 tttacccatc cgcagtgcgt cttgttcata cagacgaaca accgggttag acaccacgtt
    16981 cggatgcagc gggttagtgc ccagcggcgt ttcaatcggc tcgtagtgtt ccgggaacgg
    17041 accttccgcc attttgttga tggcaaacag gcgtcccatc ccttccggct gcatgataaa
    17101 cggcccggtt ggcgtacccg gtgcggcatt gccgaagtca ggaatatcgt tacccgtcca
    17161 cttgctgccg ttccactgga tcagcatccg tttcggatcc cacggtttac cgttgatatc
    17221 cgccgaagca cggttgtaca gcacgcgacg gttgagcggc cacgcccagg cccatcccag
    17281 cgtattcccc agaccggacg ggtcggagtt atcgcggtta gccatctggt tgccctgctc
    17341 tgtccagcta ccggtgtaga tccagcaaga agatgcggtt gtaccgtcat cacgcagatg
    17401 cgcaaagcta ctcagcaact gacctttctt cgcaataagc acgccattag cgtcatagag
    17461 atcttccagc gcgtagccgt tgttctcttt agccacttcg tcagattgcg gttcgtgcgg
    17521 ctgcttgtag ttccagctca tcttcatcag cgattctacg cctttaccac cttcggcctg
    17581 gtacagctcg cgcagatgat ggtagatacc cgccagaatt tcgccgtcgt tacgcgcttc
    17641 gcccggcgcg tcctgacctt tccagtgcca ctgcaaccag cgaccggagt tagcgataga
    17701 accatcttct tcggcaaagc aggtcgaagg cagacggaat acttcagtct gaatagacgc
    17761 cggatcgaca tcgttcgact cgccgtggtt ctgccagaag gtagaggttt cagtcaccag
    17821 cggatcgata accaccatgt acttcagctt gctcaggcag ctcaccactt tgtttttgtc
    17881 cgggaaggac gcaaccgggt taaagccctg gcagaaataa ccggtgactt tgccttcgtc
    17941 catcatgttg aaatacttga tgacgtcgta ggtctggtcc cacttcggca gccagtcata
    18001 gccccagttg ttctctttct gcgcggcatc gccatagaaa gatttcatca ggctaacgaa
    18061 gaacttcgga tagttgctcc agtagttcac ctgatcagcc agcgtcgctt tcggcgtgtt
    18121 cgcttccaga tacgactgca aatcaacctg tttttctgac ggcagcgtca gataacctgg
    18181 caggctggta gagagcagac ctaagtcagt cagaccctga atgttggagt gaccacgcaa
    18241 tgcattcacg ccgccaccgg ccatacccat gttaccgagc agcaactgga tcatcgccat
    18301 agtacggatg ttctgcgcac ccacggtgtg ctgcgtccag cccagcgcgt acaggaaggt
    18361 ggttgtgcga tccggtgcgc tggtggaggc cagcacttca cacactttca ggaagtcggc
    18421 ttttggcgta ccacagatgt tttcaacaac gtccggcgtg tagcgggaaa cgtgcgcttt
    18481 cagcaggttc cacacacagc gcggatgagt cagtgtttca tcgcgtttcg catagccgtt
    18541 ttcatcgaac tgatagttcc aggacgattt atcgtattgc cgtttttcag cgtcgtagcc
    18601 gctgaacaga ccgtcttcga aagcaaaatc atcacgcacc agcaggctgg cgttggtgta
    18661 atgcttaacg tattcggcgt tgattttgtc gttttcgatc aggtagcgca aaacgccaga
    18721 caggaacgta atgtccgtac cggaacgaat aggtgcgtaa atatccgcca cagaagcggt
    18781 acgcgtaaaa cgaggatcga caacgatcaa ggttgcatcg ttgttgtttt tcgcttccat
    18841 cgcccagcgg aaaccgacgg gatgcgcttc agcagcgtta ccgcccatta ccattacgac
    18901 gttagcgttt ttgatatcca cccagtggtt ggtcatcgca ccgcgaccaa atgttggagc
    18961 aagacttgct accgttggtc cgtgtcagac gcgcgcctgg ttgtctaccg ccagcatccc
    19021 gagggagcgg gcaaatttct gtgtcagcat cccggtttcg ttgctggcac cggaggcaca
    19081 cagcataccg gtagaaagcc aacggtttac cgttacgccc tgctcgttct tttcaataaa
    19141 gttagcgtca cggtcagctt tcatcagctt tgcaatacgg gagaatgctt cttcccagct
    19201 aatgcgctgc catttgtcag aacctggcgc acgatattcc gggtagcgca gacggttttc
    19261 gctgttgacg taatccagca aaccggcccc ttttgggcac agcgcaccac ggcttaccgg
    19321 atgatccggg tcaccttcaa tgtgataaat cgcttctctg gcgtttttcg cgccatcacc
    19381 caggctatac atcaatagcc cgcaacctac ggaacagtat gtgcaggtgt tacggatctc
    19441 tttagcgcgt aataatttgt agtttcgcgc ctgagccagt gcttgcttcg gggcaaagcc
    19501 caatgccgct accgttgttc cagccatacc gcccgcgcag attttaaaaa attgtctgcg
    19561 actgacgtcc attgctttcc tcttttttca ggggtattac tgcgcgagga aactaacagt
    19621 aaaattctga atctttttgc gcgaaatcaa aaagaacatc tgtttgccac aaaataatcc
    19681 gcctgcgcaa attgacctac ctcaatagcg gtagaaaaac gcaccactgc ctgacaggcc
    19741 agttaaaaaa atgctataaa attcagctta atttttaacg gcaagagaga caaaacagcg
    19801 agcatgacac gacaaaaagc aacgctcata gggctaatag cgatcgtcct gtggagcacg
    19861 atggtaggat tgattcgcgg tgtcagcgag gggctcggcc cggttggcgg cgcagctgct
    19921 atctattcat taagcgggct gctgttaatc ttcacggttg gatttccgcg tattcggcag
    19981 attccgaaag gctatttact cgccgggagt ctgttattcg tcagctatga aatctgtctg
    20041 gcgctttcct tagggtatgc ggcgacccgt catcaggcga ttgaagtggg tatggtgaac
    20101 tatctgtggc ccagcctgac aattctcttt gccattctgt ttaatggtca gaaaaccaac
    20161 tggctgattg taccaggatt attattagcc ctggtcggca tctgctgggt gttaggcggt
    20221 gagaatgggt tacattatga tgaaatcatc aataatatca ccaccagccc attgagttat
    20281 ttcctggcgt ttattggtgc gtttatctgg gcagcctatt gcacagtaac gaataaatac
    20341 gcacgcggat ttaacggaat taccgttttt gtcctgctaa cgggagcatg tctgtggatt
    20401 tactattttc tcacgccaca accagaaatg gtatttagca cgcccgtcat gattaaactc
    20461 atctctgcgg catttacctt aggatttgct tatgctgcat ggaatgtcgg tatattgcat
    20521 ggcaatgtca ccataatggc ggtaggctcg tattttacgc ctgtactttc ctcagcgctt
    20581 gcagccgtgc tgctcagcgc cccgctgtcg ttctcgttct ggcaaggcgc gctaatggtc
    20641 tgcggcggtt ccctgctctg ctggctggcg acacgtcgtg gttaaaataa ttgccggg
//
LOCUS       DAZKIV010000079        20690 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZKIV010000079 DAZKIV010000000
VERSION     DAZKIV010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20690
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.079"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(182..1186)
                     /gene="argF"
                     /locus_tag="ACHOT6_002796"
     CDS             complement(182..1186)
                     /gene="argF"
                     /locus_tag="ACHOT6_002796"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFP9947984.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1348..1764
                     /gene="rraB"
                     /locus_tag="ACHOT6_002797"
     CDS             1348..1764
                     /gene="rraB"
                     /locus_tag="ACHOT6_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFP9947985.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1810..2313)
                     /gene="yjgM"
                     /locus_tag="ACHOT6_002798"
     CDS             complement(1810..2313)
                     /gene="yjgM"
                     /locus_tag="ACHOT6_002798"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9947986.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2506..3702
                     /gene="yjgN"
                     /locus_tag="ACHOT6_002799"
     CDS             2506..3702
                     /gene="yjgN"
                     /locus_tag="ACHOT6_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFP9947987.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3758..6613)
                     /gene="valS"
                     /locus_tag="ACHOT6_002800"
     CDS             complement(3758..6613)
                     /gene="valS"
                     /locus_tag="ACHOT6_002800"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFP9947988.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6613..7056)
                     /gene="holC"
                     /locus_tag="ACHOT6_002801"
     CDS             complement(6613..7056)
                     /gene="holC"
                     /locus_tag="ACHOT6_002801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFP9947989.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEITWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7190..8701)
                     /gene="pepA"
                     /locus_tag="ACHOT6_002802"
     CDS             complement(7190..8701)
                     /gene="pepA"
                     /locus_tag="ACHOT6_002802"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFP9947990.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8855..8905
                     /gene="ytgA"
                     /locus_tag="ACHOT6_002803"
     CDS             8855..8905
                     /gene="ytgA"
                     /locus_tag="ACHOT6_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFP9947991.1"
                     /translation="MSIIILATISTTQENK"
     gene            8968..10068
                     /gene="lptF"
                     /locus_tag="ACHOT6_002804"
     CDS             8968..10068
                     /gene="lptF"
                     /locus_tag="ACHOT6_002804"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9947992.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10068..11150
                     /gene="lptG"
                     /locus_tag="ACHOT6_002805"
     CDS             10068..11150
                     /gene="lptG"
                     /locus_tag="ACHOT6_002805"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9947993.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11269..12771)
                     /gene="yjgR"
                     /locus_tag="ACHOT6_002806"
     CDS             complement(11269..12771)
                     /gene="yjgR"
                     /locus_tag="ACHOT6_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFP9947994.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASIEQQNNPPAKGKEIAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12849..13847)
                     /gene="idnR"
                     /locus_tag="ACHOT6_002807"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             complement(12849..13847)
                     /gene="idnR"
                     /locus_tag="ACHOT6_002807"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HFP9947995.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSEHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLEKRVRHKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            complement(13914..15233)
                     /gene="idnT"
                     /locus_tag="ACHOT6_002808"
     CDS             complement(13914..15233)
                     /gene="idnT"
                     /locus_tag="ACHOT6_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HFP9947996.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGHYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            complement(15298..16062)
                     /gene="idnO"
                     /locus_tag="ACHOT6_002809"
     CDS             complement(15298..16062)
                     /gene="idnO"
                     /locus_tag="ACHOT6_002809"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HFP9947997.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGFLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            complement(16086..17117)
                     /gene="idnD"
                     /locus_tag="ACHOT6_002810"
     CDS             complement(16086..17117)
                     /gene="idnD"
                     /locus_tag="ACHOT6_002810"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HFP9947998.1"
                     /translation="MQVKTQSCVVAGKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTEMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQAGELQGKRVFISGVGPIGCLIVSAVKTLGAAEIVCADVSPRSLSLGKEMGADVL
                     VNPQNDDMDHWKAEKGYFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLRGSFRFTSEFNTAVSWLANGVINPLPLLSAEYPFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            17334..17897
                     /gene="idnK"
                     /locus_tag="ACHOT6_002811"
     CDS             17334..17897
                     /gene="idnK"
                     /locus_tag="ACHOT6_002811"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFP9947999.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLECLNDASYSLYKKNETGFIVCSSLKKHYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQNRICAKEGSASDQRCE"
     gene            complement(17901..18920)
                     /gene="ahr"
                     /locus_tag="ACHOT6_002812"
     CDS             complement(17901..18920)
                     /gene="ahr"
                     /locus_tag="ACHOT6_002812"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFP9948000.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRVDWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQELLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            19116..19200
                     /locus_tag="ACHOT6_002813"
     tRNA            19116..19200
                     /locus_tag="ACHOT6_002813"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19150..19152,aa:Leu,seq:caa)
     gene            19399..20664
                     /locus_tag="ACHOT6_002814"
     CDS             19399..20664
                     /locus_tag="ACHOT6_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9948001.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLSYRF
                     GGKEKMLALGKYPEVSLVDARGRRDEARKLLANGVDPSENKKAVKVEHEQEVITFEVV
                     AREWHASNQKWSASHSARVLKSLEDNLFAAIGKRNIADLKTRDLLVPIKAVESSGRLE
                     VAARLQQHTTAIIRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLQRIY
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEVDFDTAMWTIPGEREPLEGVKHSQRG
                     SKMRTPHLVPLSRQALTILEKIKSMSGNRELIFVGDHDPRKPMSENTLNKALRVMGYD
                     TKTEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERMLM
                     LQWWADYLDANRMAAITPFDYAKINSSFR"
BASE COUNT         5007 a         5230 c         5138 g         5315 t
ORIGIN      
        1 ggaatggcaa aatcaatata tatgaatata ttaataaaga acagttagaa aaattaaatc
       61 ctccggaaga tttacgtata gcaatagaaa aatatggatg gaataattga actattactg
      121 cataaaataa tgtgatgccg ggatgttttt gtatttcccg gcatctttat agcgatatca
      181 attatttact gagcgtcgcg accatcaccg ctttgatggt atgcatgcgg ttttccgcct
      241 gatcaaaaac aatgctggcg gcagattcga agacctcatc agtcacttcc attccgccat
      301 gtaggccaaa ctcttccgcc attttcttac caagcgtcgt ctggtcgtca tgaaacgcgg
      361 gcaggcagtg gaggaatttg acctccgggt taccggtcaa ctgcatcatc ttgctgttca
      421 cctgataatc acgcagtaat gcaatccgtt ctgcccattt ctcttttgct tcccccatcg
      481 acacccatac atcggtatag ataaagtcag caccttcaac tcctttggct acatcttccg
      541 tcagcgtaat attcccgcca ttttgctgtg ccagggcgcg gcattccgta accagcgcag
      601 cttccggcca gcatgcctgt ggcgcgacca gacgcaaatc cagaccggta agcgccgcag
      661 cttcgagcat cgaattgccc atgttgttac gtgcgtcacc tgcatagacc agcgtcattt
      721 cgttgaatgc tttaccgggt aaatgctcct gcatggtgag gagatccgcc agcaactgcg
      781 tggggtgaaa ctcattggtc aggccgttcc ataccggcac gccagcgtat tccgccagtg
      841 tttcgacaat ctcctgacca tagccgcgat actgaatacc gtcatacatg cgacccagca
      901 cgcgggcagt gtctttaatc gactctttat gaccaatctg actgccgctt gggccgagat
      961 aagtaacgcg agcaccctgg tcatatgcgg caacttcgaa agagcatcgg gtacgggtcg
     1021 agtctttttc gaagatgagc gcgatgtttt taccagtgag tttggcttct tctttgccgc
     1081 ttttcttatc ggctttcagc ttcgcggcta actgcagcag gctgttgagt tcagctggcg
     1141 tgaaatcgag taatttcagg aaatgcttat gataaaaccc ggacatagat ccctcctgtg
     1201 gctaacgcct taatgaatta aaattcaatt tatatgggtg attattcatt tgcaagtcta
     1261 aagcataaat ctttgtcaca aaggtggagg caatgtcagt ggtgtgtgac aataagagta
     1321 tcggcaggac attaagagga atgagccatg gcaaacccgg aacaactgga agaacagcgt
     1381 gaagaaacac gtttgattat tgaagaatta ctggaagatg gcagcgatcc ggacgcgctg
     1441 tacaccatcg aacaccatct ttccgcagac gatctggaaa ccctggaaaa agcagcagtt
     1501 gaagcgttta aactcggtta cgaagtgacc gatccagaag agctggaagt tgaagatggt
     1561 gatatcgtga tttgctgcga catcctcagc gagtgcgcgt tgaatgccga tctgatcgat
     1621 gcccaggttg aacaactgat gacgctggcg gagaaatttg acgttgagta cgacggttgg
     1681 ggcacttact ttgaagatcc caatggcgaa gacggcgacg atgaagattt tgtcgatgaa
     1741 gacgatgacg gggttcgcca ctaattaatt gattaaaatg cccgatagca cacactagcg
     1801 ggcatttttt tagagttcgc gtagcatccg cacttcacaa tctacatggc ccgtgcagcc
     1861 aagtgcatag tcgatatgtt gaaaacctaa atgttcatac aaaccaatcg cttctttcaa
     1921 aaaagcggtc gtttccagat agcagcgttt gaagcccatc tctcgtgcct gctccatcgc
     1981 cattaaggcc agttttttcg ccagcccttt accgcgaata gccggaagaa aatacatctt
     2041 ctgcagttcg caaatgtccg actcactccc ggttaatggc gcaatcccac cgccgccgac
     2101 cacttcacca tcgtactcaa ccacccaata tgcatgaccc ggctgactat acacttgata
     2161 caactcgtcc aggttcggat cggcgacggt gtagccttta tcagcggtaa gaccgtattc
     2221 ggcggatacc tgacgaatga cgcgggcgat ggcgggatta tcctgctgcg tcaggcgacg
     2281 cattacaggt gattgcggcg caatgttatt catagtttaa cccaataaaa ataaagtaat
     2341 tatacatttg tttaatacca ctctcttctt acgtgcgcaa gcgacttata aagtaataaa
     2401 gtttttgcca gcctattact gataagagta ttgtaacgtg gcagaacatt ttactgtctg
     2461 tatatataaa ttatatcaat ggatttttta attcagggaa tttttatggc tcaagttatt
     2521 aatgaaatgg atgttccgtc ccattcgttt gtttttcatg gtacaggtga gagatatttt
     2581 cttatttgtg tggtgaatgt gttgttaacg attataacgc taggtatcta tttgccatgg
     2641 gcattaatga aatgtaagcg ttatctctat gctaatatgg aagttaacgg acaacgattt
     2701 tcttatggaa ttactggtgg gaatgttttt gttagttgtc ttgtttttgt tttttgctat
     2761 ttcgcaatct taatgacagt gtcagcagat atgccacttg ttggctgtgt tttgactttg
     2821 ttactgttgg ttttgcttat atttatggca gcaaaaggac tgcgttatca ggccttgatg
     2881 accagtctca acggcgtaag atttagtttt aattgctcta tgaaagggtt ctggtgggta
     2941 acctttttct tgccgatttt aatggccatt gggatgggga ctgttttctt tatctcgaca
     3001 aagatgctac atgccaatag ttcaagtagt gttattatat ctgtggttct gatggcaata
     3061 gttggtattg tttccattgg tatttttaat ggtactttat atagcctggt aatgagtttt
     3121 ctctggagca ataccagttt cggtatacat cgtttcaagg tgaaattaga tactacgtat
     3181 tgtataaaat atgccattct cgcattttta gctttattac cttttctcgc tgttgctggt
     3241 tatattatct tcgatcaaat attaaatgcg tatgatagtt ctgtgtatgc aaatgatgat
     3301 attgagaatt tacagcaatt tatggaaatg caacgtaaaa tgataatcgc gcagttaatc
     3361 tattattttg ggattgctgt tagcaccagt tatttaacgg tgtcgttgcg aaatcatttt
     3421 atgagcaacc tgtcactgaa tgatgggcgt attcgttttc gctcaacttt aacgtaccac
     3481 ggtatgcttt atcgcatgtg tgcgttggtg gtgatatccg ggattacggg cggtctggct
     3541 tatccactgc tgaaaatatg gatgattgac tggcaggcaa aaaatacgta tttgctgggc
     3601 gatttggatg accttccttt aatcaataaa gaagaacaac cagataaagg cttcttagcc
     3661 aggatttcac ggggaattat gccttcttta ccatttctgt aagagataaa aaaggccgga
     3721 gcatgctccg gccttcgttt tcattactgt gttttgatta cagcgcggcg ataacagcct
     3781 gctgttcaat cagcttcgct ttcgcttctg catagccttc cagcttttca cgctctttcg
     3841 cgatgaccgc ttccggtgcg cgggcgacaa agccttcatt cgccagcttg ttctcgatac
     3901 ggctgatttc accttcaatc ttcgccactt ctttcgccag acgcgccagc tcatcttctt
     3961 tgttgatgag gccagccatc gggatcagca gctctgcacc gtcgatgatc ttagtaacgg
     4021 aaaccggacc tttgtcatcg gcaggcagca cggtgatact ttccagacgc gccagcgttt
     4081 gcaggaagcc acggttttca tttacgcgac gttctgcatc cgcgctgcaa ccacgcagta
     4141 gcagctccag cggtttgccc ggcgcgatgt tcatttctgc acggatgtta cgtaccgcaa
     4201 cgatcgcctg cttcagccac tcggtgtcgg ccagtgcggc ttcatcaacc tgagatgcat
     4261 cgtactgcgg gaacggctgc agcatgatgg tgtcggcagt gataccgcaa agtactttca
     4321 cacgctgcca gatggtttcg gtgatgaacg gaatgatcgg atgcgcgagg cgcagcagac
     4381 cttccagtac agtcaccagc gtatggcgag taccgcgcag ttctgcttcg gtgccaccgt
     4441 tcattaccgg cttggtcagc tcgagatacc agtcacagaa ctggttccag gtgaactcat
     4501 acagaatgcc tgcggcgata tcgaagcgga agctgtccag cgcttcgcgg tacgctttga
     4561 tggtctggtt gaactccgcc agaatccagc ggtccgccag cgacagcgtc atttcgccgc
     4621 cgttgaagcc gcaatcctga ccttctgtgt tcatcagcac aaagcggctg gcgttccaca
     4681 gcttgttaca gaagttacgg taaccttcca ggcgcttcat atcccagttg atgtcacgac
     4741 cggtagacgc cagtgccgcc agggtgaagc gcagcgcgtc agtaccgtgc ggttcaatac
     4801 cgttcgggaa ctgcttctcg gtgcgcttac ggattttgtc cgccagctgt ggctgcatca
     4861 tattgccggt gcgtttttcc agcagttctg gcagcgaaat accgtcaacc atatccagtg
     4921 ggtcaataac gttacccttg gatttggaca tcttctggcc ttcgtcgtca cgaatcaggc
     4981 cggtcatgta aacggtgtgg aacggcacct gcggtttgcc attttcatct ttgatgaagt
     5041 gcatggtcat catgatcatg cgggcaatcc agaagaagat gatgtcgaag ccggatacca
     5101 tcacgctggt tgggtggaac tgacgcaggg cgtcggtgtt ttccggccag cctagggtag
     5161 agaaggtcca cagtgcagaa gagaaccagg tgtcgagaac gtcttcgtcc tgacgcaggg
     5221 caacgtcggc accgaggtta ttttctttac gcacttcgtc ttcgttgcgg ccaacataaa
     5281 cgttacccgc ttcgtcatac catgccggga tacggtgacc ccaccacaac tgacgagaga
     5341 tacaccagtc ctgaatatcg cgcatccagg agaagtacat gttttcgtac tgcttcggta
     5401 cgaactgaat gtcgccgttc tcaaccgctt caactgccgg tttcgccagg acatcggcac
     5461 gcacgtacca ctggtcggtc agcattggtt cgatgaccac gccgccacgg tcgccgtaag
     5521 gaacggtcag gtcatgtggt ttaatttctt ccagcaggcc gagtgcgtca accgcagcaa
     5581 cgaccgcttt acgtgcagca aaacgctcca gtttctggaa ttctgccggg atctcgctgg
     5641 aataaacgtc agattcgtta cctttggtat cgaacacctg ggcactttca cggatatcgc
     5701 cgtcaaaggt caggatgttg atcatcggca gggcgtgacg tttaccgact tcatagtcgt
     5761 taaagtcgtg cgccggggtg atcttcacgc agccggtgcc tttttccatg tcggcgtgtt
     5821 cgtcgccaac gatcggaata cgacggttaa ccagcggcag aatgacatat ttgccaatca
     5881 gatctttgta acgcggatct tccgggttaa cggctacgcc agtatcgccc agcagggttt
     5941 ccggacgggt agtcgcaacc accagataat ctttaccgtc tgcggttttc gcgccgtcag
     6001 ccagcggata gcggatgtgc cacatcgaac ctttcgattc gcggttttcc acttctaggt
     6061 cagagatagc ggtgcgcagt ttcgggtccc agtttaccag gcgcttgcca cggtaaatca
     6121 ggtcttcttt atacagacga acgaaaactt ctttcaccgc attggacagg ccttcgtcca
     6181 tggtgaagcg ttcacgctcc cagtccacgg agttgccgag acggcgcatc tgacgggtaa
     6241 tggtgccgcc ggattccgct ttccattccc agattttgtc gatgaaagct tcgcggccgt
     6301 agtcgtgacg ggttttacct tcttctgcgg caatcttgcg ctcaacgacc atctgggtag
     6361 cgatcccggc gtggtcagta ccgacctgcc acagggtgtt tttgccctgc atgcgctgat
     6421 agcggatcat ggtatccatg atggtttgct ggaaggcgtg acccatatgc aaactgccgg
     6481 tgacgttcgg cggcgggatc atgatgcaga aactttcctg gctttcatcg ccattaggct
     6541 taaagtagcc ctgcttttcc cagtgctcgt aaagcggctg ttcgatatct tgtgggttat
     6601 atgtcttttc cattatttcc aggttgccgt attcaggttg aaaccagcca cgcggtaggc
     6661 tttatagcgt tcgcgcgcca gttgtttcag agaatcttca taaggaacga agtctaccac
     6721 ttctgtgaaa gcggtggcaa aatctgcaaa gcttgttcgc agactaatca atatatcgcg
     6781 cgggctgctg ctgcgttttt gcggccaggt gatctccacc ggcgcactgc cgcgcggtcc
     6841 ttctcccgct aaattatgcg gaacaaagct ttctgccgga cgcgcccaca gggcttcatc
     6901 cagccggtaa gcctgctttt catcttcaca ggcgatgagc acgcgcttac cactgcgcca
     6961 acgttctgcg gcaatttcac acaccagctg ctcaacggcg cttaagccat cgacggtggt
     7021 gtcattgtcc agaaggtaga acgtcgcgtt tttcataaat ggggcttctt gtggtggatt
     7081 taaatattgc tgcattcctc accctaaaag atgagcaatg aatttctatt gcgctcgatc
     7141 ggttccctct cccttaggga gagggttagg gtgagggtaa aacgcaaact tactcttcgc
     7201 cgttaaaccc ggcgcggttt aacaggaact gtgccagcaa cgctaccgga cgaccggttg
     7261 cgcctttcgc tttaccagaa cgccaggcgg taccggcgat atccaggtgc gcccagttgt
     7321 acttacgggt aaagcgtgac aggaagcaac ctgcggtaat cgccccacca ggacgaccgc
     7381 caatgttcgc catatcggca aaattggact ccagttgttc ctgatactcg tcacccagcg
     7441 gtaagcgcca tgcgcggtca ccggattgtt cagacgcggc aatcagttca tgggccagcg
     7501 gattgtggtt cgccatcaga ccagtaatgt gatgacccag cgcgatcacg caggcaccgg
     7561 tcagcgtcgc cacgtcaatc accgcttccg gctcaaaacg ctcaacgtaa gttaacacgt
     7621 cgcacagtac caggcggcct tcagcgtcgg tgttgagcac ttcaacggtt tgaccagaca
     7681 tggtggttaa cacatcgccc ggacgatagg cgcgaccgcc tggcatgttt tcacaacctg
     7741 ccagcacgcc gataacatta atcggcagtt gtagctccgc gaccatccgc atcacgccgt
     7801 aaaccgccgc cgcgccgcac atatcgtact tcatctcatc catgccttct gaaggcttga
     7861 tggagatacc gccggagtcg aaggttaaac ctttacccac cagcacgatt gggcgcgcat
     7921 cttccgacgc gttgcctttg tactcaatca ccgacatcag tgattcgttt tgcgaaccct
     7981 gaccaaccgc cagataggaa tgcatcccca gctctttcat ctgctgttcg ccgataacgc
     8041 gggtgatgac attcttgctg tagctgtcag ccagctggcg cgcttgtgaa gcgaggtaag
     8101 cggcgttaca gatattcggc ggcatattgc cgagatcttt tgctgcttta atcccggcgg
     8161 caatcgccag accgtgctgg atcgcgcgct caccgctggt cagttcacgg cgggtcggca
     8221 cgttgaacac cattttacgc agcggacgac gcggttcgct cttgttcgtt ttcagctgat
     8281 cgaaactgta gagcgtctct tttgctgtct cgacagcctg acgcactttc cagtagttgt
     8341 tacggccttt aacgtgcagt tcagtcagaa agcagaccgc ttccattgag ccagtatcat
     8401 tcagcgtatt aatggttttc tgaataacct gcttgtactg acgctcatcc agctcacgtt
     8461 ctttgccgca accaataagg agaattcgct cggaaagtac attcggaaca tggtgcagca
     8521 acaatgtctg ccccggtttt ccttccagtt cgccccgacg tagcagggcg ctgatgtacc
     8581 catcgctgat tttatcgagc tgttctgcaa tcggagaaag gcgacgtggt tcgaagacgc
     8641 ccacgacgat gcaggcactc cgctgtttct ccgggctacc gctttttaca ctaaactcca
     8701 tgcactacgc tcctgaatct taaagacaac ggcggtggct acagatagaa ttgcaagctt
     8761 tcgtaactca tgtccgctgt tgcgatgact tcgtgttaat cttaacgtta ttacggcatt
     8821 ggcacgtcag aacaaaatct gagaggtgaa tccgttgagt ataattatct tagcgacgat
     8881 ttcgacgact caagagaata aatgacgttt aagccatgaa acaagctaaa atcctgcaaa
     8941 agacgagttt ttacgggcgt atttaaagtg ataatcataa gatatctggt gcgggagacg
     9001 ctcaaaagcc agctggcgat actcttcatc ttgcttttga tcttcttctg tcaaaagtta
     9061 gtgaggatcc tcggcgcagc ggttgacggc gatattccgg cgaatctggt gctctccctt
     9121 ctcgggttgg gcgtgccgga aatggcgcag cttatcctgc cattaagcct gttcctcggg
     9181 ctgctgatga cgctgggcaa actgtatacc gaaagtgaaa ttacggtaat gcatgcctgc
     9241 ggcctgagca aagcggttct ggtgaaagcg gcaatgatcc ttgcggtatt cacggcaatc
     9301 gtcgcggcgg ttaacgtgat gtgggcggga ccgtggtcat cgcgtcatca ggatgaagtg
     9361 ttagcagaag cgaaagcgaa ccctggcatg gcggcgctgg cgcaagggca attccagcaa
     9421 gcgactaatg gcagttcggt gctgttcatc gaaagcgttg acggcagcga tttcaaagat
     9481 gtgttcctcg cgcaaattcg accaaaaggt aatgcacgtc cttctgtggt ggtggccgat
     9541 tccggacatt taacccagct gcgcgacggc tcccaggtcg tcactctcaa ccagggaacg
     9601 cgcttcgaag gcactgcact gttacgtgat ttccgcatta cggatttcca ggattatcag
     9661 gcgatcattg gtcaccaggc ggtggcgctc gacccgaacg ataccgacca gatggacatg
     9721 cgcacattgt ggaacactga caccgatcgt gctcgcgcag aactgaactg gcgtatcacg
     9781 ttggtgttca ccgtgtttat gatggcactt atggtcgtac cgctgagcgt ggttaacccg
     9841 cgtcaggggc gcgtactatc gatgctgcca gccatgctgc tgtatctgtt gttcttcctg
     9901 atccagacct ccctgaaatc gaacggcggt aaaggtaagc tggacccgac gctgtggatg
     9961 tggaccgtta acctgattta tctggctttg gcgattgttc tcaacctttg ggacaccgtg
    10021 ccggtccgcc gcctgcgcgc cagtttttcg cgtaaaggag cggtgtgatg caaccttttg
    10081 gcgtacttga ccgctatatc ggtaaaacta ttttcaccac catcatgatg acgctgttca
    10141 tgctggtgtc gctgtcgggc attatcaagt ttgtcgatca gctgaaaaaa gccgggcagg
    10201 ggagttacga cgcgttaggc gcaggaatgt ataccttgct gagcgtgccg aaagatgtgc
    10261 aaatcttctt cccgatggca gctctgcttg gggcgttgct tggtcttggg atgctggcgc
    10321 agcgcagcga actggtggtg atgcaggctt ctggtttcac ccgtatgcag gttgcgctgt
    10381 cggtgatgaa aaccgccatt ccgctagtct tgctgacgat ggcgattggt gaatgggtcg
    10441 cgccgcaggg cgagcagatg gcgcgtaact accgtgcgca ggcgatgtac ggcggctcgt
    10501 tgctctctac ccagcaaggc ttatgggcga aagatggcaa caacttcgtc tacattgagc
    10561 gggttaaagg tgacgaagag ttaggtggca tcagcattta tgcctttaac gaaaatcgtc
    10621 gtctgcaatc cgtacgctat gccgctactg cgaagtttga cccggaacat aaagtctggc
    10681 gtctgtcgca ggttgatgaa tctgatctga ccaatccgaa acagatcacc ggttcgcaga
    10741 cggtgagcgg cacctggaaa accaacctca cgccagacaa actgggcgtg gtggcgctgg
    10801 acccggatgc actctcaatc agcggtttgc acaactacgt gaagtatctg aagtcgagcg
    10861 gtcaggatgc cggacgttat cagctcaata tgtggagcaa aatcttccag ccgctatccg
    10921 tggcggtgat gatgctgatg gcgctgtcgt tcatctttgg cccactgcgt agcgtaccga
    10981 tgggagtgcg tgtggtcacc ggtatcagct tcggttttgt cttctacgta ctggaccaga
    11041 tcttcggccc gctgacgttg gtttacggca tcccgccgat tatcggcgca ctgttgccaa
    11101 gcgccagctt cttcttaatc agcctgtggc tgttaatgag aaaatcgtaa ccaagcaagg
    11161 gagcgaaagc tccctttcac attgttgata aagtcaaata cgagttcgat cggtcccctc
    11221 gcccctttgg ggagagggtt agggtgaggg ggacagatcg cgcctgactt accttcttct
    11281 cccccccagc aaactcccca acatcccgcg tacaatctga ttcgtcacct ggcgagcggc
    11341 gcttttggcc attgtctgca ccacaccatc tttcttcccg ccgcgtggtc cggtagtgcc
    11401 gaacaaaata tccttcaatc caccaagaat gccgtcatct accgctatct ctttcccttt
    11461 cgctggagga ttattttgct gttcaatact cgcctgtacg cctttttgca acatctcata
    11521 ggcggattcc cggtccacct catcctcgta cttgccatac accggagagt gattaatcaa
    11581 gccattacgc tcatcttccg tcaccggtcc catccgcgaa caaggcgcaa tcaccatcgc
    11641 ccgttccacc actgaaggac ttcctttcac atcaagaaac gagattaacg cctcgccggt
    11701 ccccagttcc tggattgcct tttcggtatc aaatgccgga ttggcccgca tggtttgcgc
    11761 cgcagctttt accgctttct gatctttggg cgtaaaagca cgcaaagcgt gttgaacgcg
    11821 attaccgagc tgcccgagca cattatccgg aatatcagac gggttttgcg aaacgaacca
    11881 gacgcctacg ccttttgagc gaataagccg tatcacctgc tcaatcttat ccagcagtac
    11941 ctgcggtgca tcgttaaaca gcagatgtgc ctcgtcgaag aaaaacacca gttttggttt
    12001 ctccagatcg cccgcttccg gcaattgttc atacaactct gaaagcatcc acagcaggct
    12061 ggcggcgtac agtttcggca tctgataaag cttctcggcg ctgaggatat tgataacgcc
    12121 tttaccgttg gtatcggtgc gcatccagtc tttgatatcc agcatcggct cgccaaagaa
    12181 gtgtgctgcg ccttgctgtt ccagcgacaa cagtccgcgc tggatggcac caaccgatgc
    12241 gctactgata ttaccgtact gattctggaa agatttggcg ttatcgccga tgtactgggt
    12301 aatcgcccgc agatctttaa agtcgagcag caacaatccc tgatcgtcag caatacggaa
    12361 gatgatattc agcacgccag attgcacatc gttgagattc aacagccgcg ccagcaacag
    12421 cggccccaga tccgaaaccg tcgcccgcac cggatggcct ttctcgccaa agatatccca
    12481 caccaccacc ggattagcat gcggttgcca gtcattgacg ccgatatttt taagccttgc
    12541 gagtagtttt tccgatgccg ttcctgcctg cgcgatgccg gtcagatcgc ctttcacatc
    12601 ggccataaac accggaacgc cgatttccga caatgactct gccagttttt gcagcgtaac
    12661 ggttttaccc gtccccgttg cgccagtaat cagcccgtga cggttagcca ttcccggcag
    12721 taaaaacagt tctgtgtccg gcgtgcgggc aattaacagg ggttcgctca tgagatatcc
    12781 tccagtttcc tgcctggagt ataggcaacg tccggcaaag agaatacgtg taatgaagag
    12841 aaaataacct aaagcgtgtt gccgtgataa atctgatacc cgaggtcaac agtgttgtgg
    12901 ttgtgatcgt tatttttaat tttgctcagc aacatttggg cggccatccg accaatatca
    12961 aaacggggag taattacgct ggcgaggctt gggatcattt gcctgccaat ctccagccca
    13021 tgaaaacccg caatggaaat ttgttctggc acggcgaggt ttcgctcgcg gcacagcaga
    13081 agcgcaccca tcgcaatatc gtcattggtg caaaatacac cgtctaaatc cgggtttgcg
    13141 ctaagggcat cacgcattaa ctgcattcct aaatgaatgg atgagatggc gcgtggattc
    13201 atgcgtaatg gggaaagatt atgcagcatc atcgcatcgc aatacccctg ataacgctgt
    13261 tcatcgcggg tgtcatcttt ggaaccgaga tacagaattt tgtgcctgac tcgcttctcc
    13321 agcatggtac acaccatgtc aaaagcggcc tgccgattat caaaaccgac ctccatatcc
    13381 agccgttctc cctgtacatc catcaattcc acgaccggaa tggtggctga acgcagaaat
    13441 ttcaccgtcc tgatggtgtg atatttttcc gaaagtataa tcccgtcaat gttataagag
    13501 agcaggttaa tcaccgactc ttcttcggaa tcgcgatcgt aattgtaatt agcaataagg
    13561 gtctgataat tatgctcaga ggtgacggat tctattcccg cgagaatgtc agcgaagagt
    13621 tgattttgaa atgacggaat caagataccg agggtataac tttgtgcatt caacaacatg
    13681 ccgggagcgc gattagggat ataattgatc tcttccataa ttttggcgat gcgctcgcct
    13741 gtttcctttg ccaccttttt cggcgagcgg atataacgac tcacggtcat ttttgttacg
    13801 ccagccagcg tagcgatatc ctgtaaagaa attctgtgat tgcgcataac ttctcccaac
    13861 gtcgggggaa tcttcaatct tcccccgaaa ctacatcaca atattttatt ctttcagtgc
    13921 aatacggcgt taatggctaa cacgcccagc aaacccataa tagaaataag ggtttccatc
    13981 accgtccagg tacgcaacgt ttcaccaacc gtcagattaa aatacccttt aaatagccag
    14041 aagccagggt cgtttacgtg tgacgcgatc acgctgcccg caccggttgc cagtaccatt
    14101 aaagcgggat cggcatgggt aacgttgata atcggcaaca ccacacccgc ggtggtaatg
    14161 gccgcgacgg tggcagagcc cagagcgata cgcaacagcg ccgcaacagt ccagcacatc
    14221 aataacggcg aaagcgtagt tccggtcatt aagtgtgaaa tatagtgccc gacaccgcta
    14281 tctactaata cctgcttaaa cgcgccgccg ccagcgataa taaacacaat catcgcgata
    14341 gcgcctatag aatccccaat gatatccatg atttgctcga tggtgcgtcc attacgtcgg
    14401 cccagtgtga aaatcgcaat aacaatggca ataaacagcg caacggcagg gttaccgaca
    14461 aattcaaaga agaggcgcac ggtgttagtt ttcggtaacg taatttcaca aacggcggcg
    14521 atagccatca ggatgaccgg gatcacggca gcgaaaatac tgttccagaa ggagggcatc
    14581 tcctcttcgc taaacagatg aggattaaat aagccttccg gtggtgcttt ctcaaagcga
    14641 gttagcagtt tagaaaacag cggtcctgcg acaataactg tcggaatggt aatgataaat
    14701 ccatacagta aagtcgttcc gagattagcc tcaaagatag tcgcgatggc agtaggccct
    14761 ggatgtggcg gcagaaaaca gtgggttaca gagagcgctg ctaccattgg tacgccaaca
    14821 tacaacaggg gtaatcctga tgatgctacg atggtaaata ccaacggcaa cagcaggaca
    14881 aaacccactt caaaaaacat ggcgaggccc acaaccagac cggtgatcac tagcgcccat
    14941 tgcacgcgtt ttttaccaaa agtagcaatc agcgtagtgg cgatacgttg tgccgcaccc
    15001 gtatcagaaa tcagcttgcc taacatggcc ccgaaaccga ggatcattgc cagcccgccg
    15061 agcgtgctgc cgataccatt ttgtatagag tgcaggacgg cctgtgcatc catcccttcg
    15121 gcaaatccga cgacggcagc taccagaacg agggcaataa agccgttaac tttaaagccg
    15181 atcatcagga taagaagcag cgcgacgcct gccgcaataa tgattaatgg catagtcttt
    15241 atctcttatt agtgccagaa aagcagcgct ttcgcgctgc acgtagggta gtaggtgtta
    15301 aacagccact aacatgccgc catcaacaaa caacaggtgg ccgtttacga aatcagaggc
    15361 ttttgaagaa aggaacaccg cagcaccaat cagttcctgc ggatctcccc agcgtgcggc
    15421 gggggtccgt ttgcacaacc aggcggtgaa ggcttcgtcc tcaaccagtg ctttagtcat
    15481 ttctgttttg aaatagcccg gcgcaatacc gttgacctga atattgtggc gcgccagctc
    15541 gacgcacatg ccgcgggtga gcatttttac cgcccctttc gatgcggcat aaggggtgat
    15601 ggtgtcacgt cccagttcgc tttgcatcga gcaaatatta ataactttac ctgccttgcg
    15661 ttcaaccatg tgacgagtta ccgcttgcga taccaggaac acggcggtct ggtttactgc
    15721 gatcacatca ttccactctt gttcagggaa ctcagtaaaa ggatgacggc gctggatacc
    15781 ggcgttattc accagcacat caatggggcc gatgtccttt tcgatatgtt caacggcggc
    15841 atcaatttca tgtttatgag taacattaaa aggtgcggca acggcctgaa taccctcctg
    15901 gtggagtttt tctacagcaa gttctgcgcg ttcggcagta atatcattaa taattatttg
    15961 tgcgccatat ttacccaggc cggttgccag taaaaagcca atgccctgtg ctgaaccggt
    16021 aatcaagata ttttttcctg ccagtgaaaa tagatcgttc attgtattat tcctttattt
    16081 atctcttaga aaacaagctg gacttttgct gcctgggttt tatcaccggc gaaacgtagc
    16141 gcctcttcca ggtcagtgaa gggatattca gcactcagta aaggcagtgg attgataacg
    16201 ccattcgcca gccatgacac tgcggtatta aattcgctgg taaaacggaa agagcctctg
    16261 agtgaaatct ccttaccaat caacgtcatc attgggaatt ctgccatcgc gcctcccata
    16321 cctacctgca ccattacgcc gcgtgcacga gtgacctcca gacaggtatt cactgatgaa
    16381 ggatgaccgg acacttcaaa gctgacatcg aaatagcctt tttccgcttt ccagtgatcc
    16441 atgtcgtcgt tttgtgggtt tacgagcaca tccgccccca tctctttgcc cagcgaaagg
    16501 gaacggggac tcacatcagc acagacaatt tccgcggccc ccagtgtttt cactgcactg
    16561 acaatcaggc agccaatggg tccaacaccg gaaataaata ctcgcttgcc ctgtaactcg
    16621 ccggcctgat gtgcggcatg aatcgcgacg gctaaaggtt cggcaaaagc cataaccttt
    16681 tcgtcagctt tggccggata agggacacat tgcgacgttt cgaccatttt ataacgggta
    16741 aaaccaccat caacatgagg gaaatacatg gcactgccaa aaaaacgcat ctctgtacac
    16801 tgattctcgt tatgttcaat gcagtatttg cagtgaccgc acggcttaga cggattaatg
    16861 gctaccgttt gcccttcatg taattctgat gagtcgctat gaataacttt accgataact
    16921 tcatgaccta acaccatcgg tgcctttatc atgaaattac ctacttttcc ttcctgataa
    16981 taatgtaaat cggaaccgca aattccacct cgggttattt gtactaatgt tccattatta
    17041 ttccaatcta tcgtctgctc ggtaacggca acagttttct tgcccgcaac aacgcaggac
    17101 tgtgttttca cttgcataaa ttcctcactg gtcaggtaga cacctcggaa gcatttaagc
    17161 ggttttaatt gtcattattt gtgatgaaga tcacgtcaga aaattgttac attactatgt
    17221 tacgcataac gtgatgtgcc ttgtaattct tatcagcaga aataaaaaaa cgtgaaatta
    17281 ttatgccacc aggcgtagta tcgcagcagg taagatgatt caggagattt taaatggcgg
    17341 gtgaaagctt tattttgatg ggcgtttcag ggagtggtaa aacattaatt ggtagcaagg
    17401 ttgccgcgtt attatctgct aaatttattg atggtgacga tcttcatcca gccaaaaata
    17461 tagataaaat gtcgcagggt attccattat ctgatgaaga tcgacttccc tggctggaat
    17521 gcttaaatga tgcttcatac agtctttata aaaagaatga aacaggattt attgtctgtt
    17581 catcattaaa aaaacattat cgtgatattt tacgcaaggg tagcccccat gttcatttcc
    17641 tctggttaga tggcgactat gaaactatcc tcgcgcgaat gcagcgtcgg gctgggcatt
    17701 ttatgccggt agcgttacta aaaagtcagt ttgaggcgct ggagcgtcca caagcagatg
    17761 aacaggatat tgtgcgcatt gatatcaacc atgatattgc caatgtcacc gaacaatgtc
    17821 ggcaggctgt gctggcgata cgacaaaaca gaatatgtgc gaaagagggc agcgcctcag
    17881 atcagcgctg cgaatgattt tcaaaaatcg gctttcaaca ccacgcggta acgcgcctta
    17941 ccgtcgcgca catgctggat ggcgtcgtta attttcgaca tcgggaacag ttcggttgtc
    18001 ggcgcaacct tgctgcgggc ggcaaagcgc atcagcttac gcagttcata aggcgtgccg
    18061 gtagcagagc cagagacgct gcgatcgccc gcaattaacg taaaggccgg aacagacagc
    18121 ggcgtgagaa ccgcaccgac agtgtggaaa ttaccgccgt acgtcagcgc ctcaaaataa
    18181 ggctgccagt cgaggctgac gttcacggta ttgataatga gatcaaactg ccccgccagt
    18241 gctttcagtg cctgcggatc gcggctattc accactttat cggcacccat cgccagcaat
    18301 tcctgctctt tcgccggatt agaactaaag gccgtcacct cacatcccat tgcgtgcaga
    18361 agttttatag cgatatgccc cagcccgcca ataccaatta ccccaacgcg gctggtagca
    18421 gtgatatggt gcatcaacag tggtttaaag accgtgatac cgccgcacaa cagcggcccg
    18481 gcagattcaa tgtcgatatt ttccggcagt ggaatcaccc attgccagtc tacacgcaac
    18541 ttctcggcaa aacctccgcg attcataatt gttggcaccg caccttgctc acagttgatc
    18601 tgatttccgc taatacaggc gtcgcagtga ccacagctac gcgccgtcca gccaatcccg
    18661 acacgctgac cgacctgcaa acctttatcc tgcgcggcac tcccgagcgc caccacgcga
    18721 ccaatcacct catgcccggc aaccagcgga tattgtgaaa atccccattc gttatcgatc
    18781 atcgacagat cggaatggca gatcccgcag taatccacct gcacttcaac atcttgtggc
    18841 ttcagctcac cgggatcgta ctcataaact tccagttcgc cgcccgcttc ttttgcggca
    18901 tagcttttta tcattgacat tttttggtcc ttctctggtg tggtctggga agtgtagagc
    18961 atggcagggc ggggtgcatg gagtgtgaca aaggttacac atcgctgtac gcaatgctga
    19021 aaatttcagc acttagcgag atgcgagcaa gctggcgctt gcatggtggc gtgcgacagg
    19081 tataatccac aacgttttcc gcatacctct tcagtgccga agtggcgaaa tcggtagacg
    19141 cagttgattc aaaatcaacc gtagaaatac gtgccggttc gagtccggcc ttcggcacca
    19201 aaagtatgta aatagacctc aactgaggtc tttttttatg cctgaaatcc agtatccgca
    19261 aggctttcct cgctttttca ctcaacctaa gtcaacctga ttcaatctac atcaacttac
    19321 tgatgcgggt acaactgcgg gtatttcccg gttcgattat gtttgtaccc acacagggcc
    19381 cttgaaagga tactcatcat ggctctgagt gatgtgaagg tccgttcggc taagccggaa
    19441 gcaaaagcct ataaacttac tgacggtgaa ggcatggttt tgttggttca ccctaatggc
    19501 tccaaatact ggcgacttag ttatcgtttt ggcggtaagg agaagatgct ggcgctgggg
    19561 aaataccctg aagtctcgct ggtcgatgcc agaggtcgcc gggatgaagc ccgtaagttg
    19621 ttagctaacg gcgtcgatcc tagtgagaac aagaaagctg ttaaggtaga gcatgagcaa
    19681 gaggtgataa cgtttgaagt ggtagccaga gagtggcacg ccagcaatca gaagtggtct
    19741 gcatcgcata gcgctcgtgt attgaaaagc ctcgaagata atctctttgc tgctatcggt
    19801 aagcggaaca ttgctgatct gaagactcgg gatctgcttg tccccatcaa agcggtggag
    19861 tcgtctgggc gtctcgaagt cgctgctcgt ttgcagcagc atactaccgc gattatacgc
    19921 tttgctgtac agagtggatt aatcgactac aacccagctc aagagattgc tggtgcggtt
    19981 gctacggcga aaagacagca tcgtgctgct ctggaactta accgcattcc tgaattgctt
    20041 caacgcatct atcactattc tggcaggccg ttaacccgac tggcggttga actcacattg
    20101 ttagttttca tccgttcaag cgagttgcgt tttgctcgct ggtcagaagt tgattttgat
    20161 actgccatgt ggacgattcc gggagaacgt gagccactgg agggagttaa gcattctcag
    20221 cgtggttcga agatgcggac tccacatcta gtacccttat ctcgacaagc tctcaccatt
    20281 ctggaaaaaa tcaaaagcat gagtggaaac cgtgagctga tcttcgttgg tgatcacgat
    20341 ccgcgtaagc caatgagtga gaatacgctt aacaaggcct tgcgagtgat gggctatgac
    20401 acgaaaacgg aagtgtgtgg acatggtttc aggacgatgg cgtgtagttc tttgattgaa
    20461 tcgggattgt ggtcgaggga tgcagtagag cggcagatga gtcatcagga acgtagctca
    20521 gtacgggcgg cttatatcca taaggcggaa catcttgggg agcgaatgtt gatgctgcag
    20581 tggtgggctg attatctgga tgcgaataga atggcggcta tcactccatt tgactatgca
    20641 aagattaata gttcttttcg ataattctag ctaagtatat gaatattaac
//
LOCUS       DAZKIV010000080        20459 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZKIV010000080 DAZKIV010000000
VERSION     DAZKIV010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.080"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..187)
                     /locus_tag="ACHOT6_002815"
     CDS             complement(<1..187)
                     /locus_tag="ACHOT6_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFP9948002.1"
                     /translation="MTRKGWLPELEGLRGLASVWVFIGHIAILTECRIPILGEPTYGV
                     DLFILLSGFLMTKNYIER"
     gene            579..1751
                     /locus_tag="ACHOT6_002816"
     CDS             579..1751
                     /locus_tag="ACHOT6_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFP9948003.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDNKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            1761..2276
                     /locus_tag="ACHOT6_002817"
     CDS             1761..2276
                     /locus_tag="ACHOT6_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFP9948004.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            2331..2633
                     /locus_tag="ACHOT6_002818"
     CDS             2331..2633
                     /locus_tag="ACHOT6_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFP9948005.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            2648..2767
                     /locus_tag="ACHOT6_002819"
     CDS             2648..2767
                     /locus_tag="ACHOT6_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFP9948006.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            2760..5837
                     /locus_tag="ACHOT6_002820"
                     /pseudo
     CDS             2760..5837
                     /locus_tag="ACHOT6_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            5834..6319
                     /locus_tag="ACHOT6_002821"
     CDS             5834..6319
                     /locus_tag="ACHOT6_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9948007.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRVEFTLSLKRVDESLSDMFGDLSSQLNNLQDTTTSALSDISKTVGGLL
                     S"
     gene            6316..7416
                     /locus_tag="ACHOT6_002822"
     CDS             6316..7416
                     /locus_tag="ACHOT6_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFP9948008.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGKDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAVRHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            7507..7725
                     /locus_tag="ACHOT6_002823"
     CDS             7507..7725
                     /locus_tag="ACHOT6_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFP9948009.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(7961..9646)
                     /gene="ybjL"
                     /locus_tag="ACHOT6_002824"
     CDS             complement(7961..9646)
                     /gene="ybjL"
                     /locus_tag="ACHOT6_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFP9948010.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9916..10293
                     /gene="ybjM"
                     /locus_tag="ACHOT6_002825"
     CDS             9916..10293
                     /gene="ybjM"
                     /locus_tag="ACHOT6_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFP9948011.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGYPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(10323..10580)
                     /gene="grxA"
                     /locus_tag="ACHOT6_002826"
     CDS             complement(10323..10580)
                     /gene="grxA"
                     /locus_tag="ACHOT6_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFP9948012.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10740..11027
                     /gene="ybjC"
                     /locus_tag="ACHOT6_002827"
     CDS             10740..11027
                     /gene="ybjC"
                     /locus_tag="ACHOT6_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HFP9948013.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            11011..11733
                     /gene="nfsA"
                     /locus_tag="ACHOT6_002828"
     CDS             11011..11733
                     /gene="nfsA"
                     /locus_tag="ACHOT6_002828"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFP9948014.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIKAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            11794..12696
                     /gene="rimK"
                     /locus_tag="ACHOT6_002829"
     CDS             11794..12696
                     /gene="rimK"
                     /locus_tag="ACHOT6_002829"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFP9948015.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            12784..13260
                     /gene="ybjN"
                     /locus_tag="ACHOT6_002830"
     CDS             12784..13260
                     /gene="ybjN"
                     /locus_tag="ACHOT6_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFP9948016.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            13611..14723
                     /gene="potF"
                     /locus_tag="ACHOT6_002831"
     CDS             13611..14723
                     /gene="potF"
                     /locus_tag="ACHOT6_002831"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFP9948017.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHVYNWSDYIAPD
                     TVANVEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            14818..15951
                     /gene="potG"
                     /locus_tag="ACHOT6_002832"
     CDS             14818..15951
                     /gene="potG"
                     /locus_tag="ACHOT6_002832"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFP9948018.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGVVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15961..16914
                     /gene="potH"
                     /locus_tag="ACHOT6_002833"
     CDS             15961..16914
                     /gene="potH"
                     /locus_tag="ACHOT6_002833"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFP9948019.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            16911..17756
                     /gene="potI"
                     /locus_tag="ACHOT6_002834"
     CDS             16911..17756
                     /gene="potI"
                     /locus_tag="ACHOT6_002834"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFP9948020.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            17816..18304
                     /gene="ybjO"
                     /locus_tag="ACHOT6_002835"
     CDS             17816..18304
                     /gene="ybjO"
                     /locus_tag="ACHOT6_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFP9948021.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            18345..19472
                     /gene="rlmC"
                     /locus_tag="ACHOT6_002836"
     CDS             18345..19472
                     /gene="rlmC"
                     /locus_tag="ACHOT6_002836"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFP9948022.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVTHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(19647..20378)
                     /gene="artJ"
                     /locus_tag="ACHOT6_002837"
     CDS             complement(19647..20378)
                     /gene="artJ"
                     /locus_tag="ACHOT6_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFP9948023.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         4933 a         5008 c         5621 g         4897 t
ORIGIN      
        1 gtcgttcaat atagttttta gtcatcaaga aacctgataa gagaatgaac aaatcaacgc
       61 cataagttgg ttcaccaaga ataggtattc tgcattctgt taaaatcgct atatggccaa
      121 taaataccca tacagaggca agtcctctta gaccttcaag ctcaggaagc catccttttc
      181 tagtcatgtg catatgcttt gaatcttcat ttgatagggg aagtgaatta tatcgccgct
      241 ttataaatat gccatcaaaa attcatatcg aatataggaa tgcactcgaa ccaatcggta
      301 agggagtgtc tgaccgaaag tgagtttgga ttatattcaa acatagacaa ataatattgg
      361 cttctggtta atatatagat attgatgtgg tttgtcacaa ttttttaaca atgcaggttg
      421 taacaagttc atcgggagca gcattatgtt gattatccct gcaagcggac aaataccgtt
      481 attttgtgtg aataacggta caactgcgct tagctgtttg tcaggcacaa tcacttcaat
      541 atagggcgaa gcctaatcca atcaggaggt tcgccactat ggctcaggat taccaccacg
      601 gggtgcgcgt tgttgaagtc aacgaaggca cccgatccat taccacggtg agcaccgcca
      661 tcgtgggtat ggtctgcacg ggcgatgatg ccgatgcaaa aatgtttcct cttaataaac
      721 ccgtgctgat cactgatgtg ctgactgcca gcggtaaagc gggtgagtcc ggcacgctgg
      781 cccgttcgct ggatgccatc gctgaccagg caaaacccgt gaccgttgtt gtgcgtgtgc
      841 cgcagggtga aacggaagaa gaaaccacga ccaatatcat cggcgcagtg actgctgaag
      901 gtaaaaaaac aggcatgaaa gctctgttat ctgcccagtc acagctcggc gttaaaccgc
      961 gcattctcgg cgtgccaggc cacgataaca aagccgttgc gactgagttg ctgagcgtgg
     1021 cgcaaagcct gcgtggattt gcttacctgt cagcgtatgg ctgcaagaca gtgcaggagg
     1081 cgatcactta ccgcgaaaac ttcagccagc gcgaagggat gctgatctgg cctgacttta
     1141 ctggctggga cacggtgctg aatgccgaag caacggcata tgccaccgcc cgtgcgcttg
     1201 gtctgcgcgc caaaattgac gagcagaccg gatggcacaa aagcctgtcc aacgtgggcg
     1261 tgaacggtgt caccggaatt tctgcagatg tgttctggga tctgcaggac ccggcaaccg
     1321 atgcaggtct gctgaaccag aacgacgtca ccacgcttgt gcgcaaggac ggtttccgct
     1381 tctggggttc ccgctgcctg agtgatgacc cgctctttgc cttcgaaaac tacacccgca
     1441 cggcgcaggt gctgatggac acgatggcag aagcacacat gtgggcggtg gataaaccgc
     1501 ttaacccgtc gctggcccgc gacattatcg aaggtatccg cgccaaaatg cgcagcctgg
     1561 tcagtcaggg ctatctcatt ggtggtgatt gctggctgga tgagtcggtg aacgacaaag
     1621 acacgctgaa agccggaaaa ctcaccatcg actatgacta cacgccagtg ccgccacttg
     1681 aaaatctgat gctgcgccag cgcatcaccg atcagtacct ggtgaatttc gccagccagg
     1741 tcagcgcgta aggggacaac atggctttac cacgcaaatt aaaacacctg aacctgttta
     1801 acgacgggaa caactggcag gggatcgttg agtcgctgac gctgccgaaa tttacccgca
     1861 aatatgagaa gtatcgcggc ggcggaatgc cgggtgcggt ggatgtggat ctggggcttg
     1921 atgacagtgc gctggacaca gaattttcca ttggtggtac tgaattgctg ctgtttaaac
     1981 agatgggtaa agccacggtg gatggcatcc agctgcgctt taccggctct attcagcgtg
     2041 acgataccgg ggaagtgcag gccgtggagc ttgtcgtgcg tggacgtcac aaagaagtgg
     2101 attccggcga gtggaagacg ggcgaaagca acaccaccaa agtgaccagt accaacagct
     2161 acgcgaagct gaccatcaat ggtgaggtgc tctatgaagt ggaccttatc aacatggtgg
     2221 aaattgtgga cggtgtggac ctgatggaag cgcaccgcaa cgccctcggc ctctgatgta
     2281 tctgaacggc gcgggattcc gcgccagaac ccaatttaca ggacaacaaa atgagcgata
     2341 agcagactga aaagaccatt caactggata cccctatcaa gcgcggtaaa acagaaatta
     2401 ccgaaattgt gctgcgtaaa ccgcagtccg gtgcgctgcg cggtacacgc ctgcaggcca
     2461 ttatggatat ggatgtgaac gcgatgatga ccgtgatccc ccgcatctcc agtccggcac
     2521 tgactgcaca ggaaattgca gagatggacc cggcagatct cactgccatg tcggttgagg
     2581 ttgtcacttt tttgttgaag aagtcggtgc ttgccggttt accgacagcc tgacggttga
     2641 cgatctggtg gctgatatcg ccaccatctt tcactggccg ccatccgtta ctgacgttat
     2701 gccgctgacc gaagtgctgg aatggcggta taaagcgatt cagagaagcg gggccaacga
     2761 tgagtgataa taacctgcgc ctgcaggtca ttcttaatac ggttgacaaa ctcacccgcc
     2821 cattccgtgc tgcacaggcc agttcgaaag agctggctgg cgcaattcag aatacccgaa
     2881 acagcctcaa agaactgaat aagcaggctg gcagaattga tgaatttcgc tagacgcgct
     2941 cgcaactagc cataacagcc aataacctga acgcagcccg cgaagaggcg gcaaaactcg
     3001 ccacacaatt tgctgccact aacaggccaa ccgccgcgca ggcaaagtta ttcagtcagg
     3061 ccaaaacacg agtacaggaa cttcagcaga cctataacgg cttgttgggg gcggtccaga
     3121 gacaacgtca ggcacttaaa gaatcaggga ttgataccag acaactcagt agtgcccagc
     3181 gagaacttaa gaaaaatgct gaagaaacaa ggcaggcact ggagggccag caaaaagcac
     3241 ttaaacgtct gggtgaacaa caggcacgga tgaacgctgc cagagaacaa tactcaagac
     3301 ggcttgaagt gcgcgatcgc atcgcaggag ccggagccac taccacggct gcagggctgg
     3361 caatgggtgc gccagtgatg gcggcagtaa aaagctatac cagcatggaa gatgccatga
     3421 aaggtgtggc aaagcaggtc aatggtctgc gtgacgataa tggcaaccgc accgcacgtt
     3481 tttatgaaat gcaggatgcc atcaaggctg ccagcgaaca gttgccgatg gaaaacggtg
     3541 cggtggactt cgctgcactg gttgaaggtg gtgcgcgcat gaacgtcgca aaccctgacg
     3601 acagctggga agaccagaaa cgtgacctgc tggccttcgc cagtacggca gcaaaggcgg
     3661 caacagcctt tgagctgcca gcggatgaac tgtcagaaag tctggggaaa atcgcccagc
     3721 tctacaaaat acctacccgc aatattgaac agctcggcga tgcgctgaac tatctggatg
     3781 ataacgccat gtcgaaaggg gcggacatca ttgatgtgat gcaacgtctg ggcggtgtgg
     3841 ctgaccgtct ggattatcgt aaagcggcgg cactgggttc cacctttctg acactgggcg
     3901 ctgcgccgga ggttgcagcc agtgcagcaa acgcgatggt gcgtgaatta tccattgcca
     3961 ccatgcaaag caagagtttc tttgaaggga tgaatctgct gaaactcaat cctgaagtga
     4021 ttgaaaagca gatgacgaag gatgcgatgg gaactatcca gcgtgtgctg gagaaggtga
     4081 acgcactgcc gcaggacaag cgtctgtctg ccatgaccat gttgtttggt aaagagtttg
     4141 gcgatgacgc ggcgaaactg gcaaacaacc tgccggaact gcagcgccag ctaaaactga
     4201 cagcgggcaa tgatgcgctc ggctccatgc agaaagaatc cgacatcaac aaagactcac
     4261 tttctgctca gtggttgctg gtcaaaaccg gagcgcagaa caccttcagc agcctgggcg
     4321 aaacgctgcg ccagccgctg atggatattc tgtacacggt gaaaagcatc acgggggcgt
     4381 tgcgccgctg ggtggaagct aacccggaac tgacaggcac actgatgaaa gtagcggctg
     4441 ttgtggctgc ggttaccgta ggcctcggca ccttagcggt ggcgctggct gcagttctgg
     4501 ggccgctggc agtgatccgt ctgggatttt ctgtgctggg catcaaaacg ttaccttccg
     4561 ttacggcagc agtaacacga accagcagcg cgttgtcctg gctggcagcc gcaccactgg
     4621 cagtgctgcg acgcgggctt gcttcatcgg gcaacgcagc gggtttactt actgctccgt
     4681 tgtcgtcttt gcgtcgcacg gcatcactga cgggaaatgt cctgaaaact gtagctggtg
     4741 cgccggttgc actgttccgg tctggattat caggtttacg tgctgttgct gtgatgttta
     4801 tgaatccact ggcagcacta cgcggcgggc tggctgccgc aggcgcggtg ctgcgagtgc
     4861 tggcatccgg cccgctggcg atgctgcgcg ttgctctgta tgccgtatct ggtctgttag
     4921 gtgctctgct tagtccgata ggtcttgtgg ttactgcact ggcgggtgtg gcgttggttg
     4981 tctggaaata ctggcaaccc atcactgcat ttctcggtgg cgtggtggaa ggattcaaag
     5041 cggcggcagg tcccatcagt gccgcattcg aaccacttaa gcccgtgttc cagtggattg
     5101 gcgacaaagt gcaggcgctg tggggctggt ttactgatct gctgacgccc gttaagtcga
     5161 actctgccga actgcagagc gcagcggcaa tggggcgacg attcggggag gcactggcgg
     5221 aagggctgaa tatggtcatg catccgctgg actccctgaa atccggcgtt tcctggttgc
     5281 tggataaact cggcattgtc agtaaagagg ctgcaaaggc gaaactgccg gaaagcgtga
     5341 cgcgtcagca acctgcgacg gtgaatgcag acggtaaagt gatgatgcca tcgggtggtt
     5401 ttccgtcatg gagatatggc tttgcgggga tgtatgacag cggcggctat attccgcgcg
     5461 ggcagtttgg catcgtcggt gaaaacgggc cggaaattgt taacggcccg gcaaacgtga
     5521 ccagccggag aaatacagct gcactggctg ccattgttgc cggaatgatg ggcgttgctg
     5581 ccgcgcctgc agagcttcca ccgttgcatc ctttggcgct tcccgcgaaa ggtggtgaag
     5641 cgatgatgag tcgcgcagcc actgtgccgc ccgttcaacg gattgaggca ccgacgcaga
     5701 tcatcatcca gacgcagcca ggacaaagtg ctcaggatat tgcgcgggag gtggcacgcc
     5761 agcttgatga acgtgaacgc aggctgaagg caaaagccag gagtaactac agcgatcagg
     5821 ggggatacga cgcatgatga tggtgctggg attgtacgtg tttatgctgc gcaccgttcc
     5881 gtatcaggaa ctgcagtatc aacgcagctg gcgacatgcg gcaaacagcc gggtaaaccg
     5941 acgtccgtcc acgcagtttc tgggaccgga caacgacatg ctgacgcttt ctggtgttct
     6001 tatgccggag ataacgggcg gcaggctgtc gttgctggct ctggagcaga tggcagaaca
     6061 gggaaaagca tggcccctga ttgaaggcag cggcacgatt tacggcatgt atgtgattga
     6121 gggactgaat cagactaaaa cggagttttt ccgcgacggt atgccgcgcc gggttgagtt
     6181 caccctgtcg ctcaaacggg tggatgaatc cctgtccgat atgttcggtg atctcagttc
     6241 gcagctgaat aatttgcagg atacgacaac gtctgcctta agcgatatca gtaaaacggt
     6301 gggagggctg ctgtcgtgaa tttcagctct gaactgctta acaaaggcaa caaaactccg
     6361 gcattcagca tcagtattga aggcaaggat atcaccactg tgctggacaa ccgcctgatg
     6421 gggcttacgc tgacggataa ccggggcttt gaagcggacc agcttgatct ggagctggac
     6481 gacgccgacg gaaaaatcgt gctgccgcgc cgtggtgcgg tcatcacgct ggcgctgggc
     6541 tggaaggggc agccgctttt cccgaaaggg gcattcacgg tggatgaaat tgaacacact
     6601 ggcgcaccgg accgcctgac tatccgggcg cgaagtgctg attttcggga aacgctgaat
     6661 acccgccgtg aaaaatcgtg gcacaagacc acagttgggg aagtggtgaa ggaaatagct
     6721 gtgcggcaca aactgaagat ggcactgggt aaagacctgt cagataaacc cgtggaacat
     6781 atagaccaga ccaatgagag tgacggcagt tttctgatgc ggctggcgcg acagtacggt
     6841 gctattgcgt cggtgaaaaa tggcaatctg ttattcatcc ggcagggaca gggtaaaagc
     6901 gccagcggta aaccactacc ggtgatcact atcacacgta aggacggcga cagtcaccgc
     6961 tttaccctgg cggatcgcgg agcttacacg ggtgtaattg ccagctggtt gcatacccgc
     7021 gaacccgcga agaaagaaag caccacggtg aagcgtaagc gcaggactaa gaagcagaag
     7081 aaagagccgg aagcgaagca gggcgattac ctggtgggga cggatgaaaa cgtgctggta
     7141 cttaatcgca cctatgctaa tcggagtaac gccgaacggg cagcgaaaat gcagtgggaa
     7201 cgcctgcaac gcggtgttgc gtcattctcg ctacaactgg cggaaggtcg ggcggatctc
     7261 tacacggaaa tgcctgtgaa ggtcagcggc tttaaacagc cgatagatga tgcggaatgg
     7321 accattacca ccctgacaca taccgtcagc ccggataacg gttttacgac cagtctggag
     7381 cttgaagtga ggattgatga tttcgaaatg gaatgattct tcgcaatgga gaacttttaa
     7441 gtttgcaaaa tggaataata cggtatcatt gttgtgaatt tagcaaaaat ggggagaact
     7501 cgaaaaatga tgatttgccc actgtgtgga agtgccgccc atactcgcag cagttttcag
     7561 gtatcttcat tgaccaaaga gcgttataac cagtgccaga acattaactg cagccatact
     7621 tttgttaccc atgaaacttt tgttcgttcg attgcaacac caaaagagtc gaatccagtt
     7681 cagccgcatc caatgaaatc aggacaggtg gcgctctctc tttgacgctg ccgccatttt
     7741 gtcgccatcg ttaaaaaaca gtgcttctaa catcatgatt ttaaacatct taaatttcag
     7801 gcaacaaaaa acccatcaac cttgaaccga aatggcgggg ttgatgggct ccacaaaatg
     7861 gggacatcaa agaaaagcag tggcactaat taagactgat gccctgcgga aaagttctgc
     7921 ggttgtgcaa aaaaatttca ttttcagggc aacttcagtt ttatcctaat cctggccata
     7981 ccatgacgat gattgtccct gccagcgtca gcaggacgtt ggcgattgcg taggtgcccg
     8041 catagcccag cgcagggatg ttactgcgag ctgtatcact gatgatctcc attgccggcg
     8101 cgcaggtgcg tgcgcccatc attgcgccga acaacagtgc gcggttcatt cgcaatacat
     8161 aagcaccgaa caagaaacag ataaccacgg gcacaagact gacaattaat ccggcaatca
     8221 acatctgacc gccaatcgcg cccaggccgt tattaatacc gctaccggcg ctcagaccaa
     8281 cgcctgccat aaacaccatc aagccgaact ctttcaccat gcttaatgca ccttgcggaa
     8341 tgtaaccgaa ggtcgggtgg ttagcacgca taaagcccag cataattccg gcgaataaca
     8401 acccggcagc gttccccatg ccgaaactga atgtgctgaa ctggaaggtg atcatcccga
     8461 tcatcagccc aataacaaag aaggcgcaaa atgccagcag gtcagtgacc tggctgtgaa
     8521 tcgagataaa gccgatgcga tcggcgatgg tttttacgcg acgggcatca ccgctgactt
     8581 gtaaaacgtc acctttgtta agcacgacgt tgtcatctat cggcatctca atctggctac
     8641 gaatgacgcg gttaaggaag caaccgtgat cggtcaactt cagttgtgcg agacgtttac
     8701 ctacagcgtt atggttttta acgaccactt cttcagtgac gatacgcatg tcgagaaggt
     8761 cacgatcgaa aacttcttta ccgttacgga agctgggatc gagtcgggca tgggcgtcgg
     8821 gatagcctac caacgctatt tcatcgccca tttgtagcac ggcatcaccg tctggatttg
     8881 ccagaatccc gttacgtcga atacgttcaa tgtagcagcc ggtttgtcga taaataccca
     8941 gttcacgcag atttttgccg tcggtccagg ccaccagctc cgggccgacg cgataggcgc
     9001 ggatcaccgg taaataaacc ttacggttgg catcagtgtc caggccacgt tcgcgggcga
     9061 tttgctgggc gctggtctgt aagtcctgat gctgcaattt cggcaagtaa cgcgcaccaa
     9121 caatcaaact caccagaccg attaaatagg ttaaggcata cccgaggctc agattatcca
     9181 gtgccagtga gagctgcctg ctttccatgc cggaatgacg cagtgtatcg ccagcaccga
     9241 ccagaaccgg tgtcgacgtc atagagcctg ctaacatacc ggccgtcagg ccaatatccc
     9301 agccaaacag cttacctaac cctaaggcga tcaccagcgc actgccaacc atcaccagtg
     9361 ctaacattag gtaatttttc ccatcgcgaa aaaaaatgga aaaaaagttc ggtccggctt
     9421 cgaccccgac gcagaaaata aacagcataa agccaagatt aagcgcgtcg gtgttaatgc
     9481 tgaaatgttg ttggcctaat aacagtgata cgaccaaaac gccaatggaa ttacccagtt
     9541 ggatcgaacc aagtcgtaac tttccgagac atagcccaag cgcgaggacc acaaataata
     9601 acagaatgta attcccattt aacaattcgg cgacgtttat attcacggag gctaacttct
     9661 tgtttactag taagctgttg aaagaagtgg taatttacga taatgttttt taccagaatt
     9721 cagggcgcag attcattcag cgcacctaaa cgatagtaaa gtaacaatat attttactag
     9781 tgtaatcaca ttaggtatca acggctatat gaattgcgtt ggcctatatt agcatggaat
     9841 gcgaagcggc tttatcttac tgaacgccac actggcgaaa aatgtgttcg atagacgcag
     9901 tgtcaggagg aacgagtgaa acataaacaa cgttgggcgg gggcaatctg ctgttttgtc
     9961 ctcttcattg tggtgtgcct ttttctggcg acgcacatga aaggcgcttt tcgggctgcc
    10021 gggtatcctg aaatcggctt gctgtttttc attcttcctg gagcagttgc cagtttcttt
    10081 tcacagcgta gagaagtcct gaaacctcta tttggcgcaa tgctggcggc accctgttca
    10141 atgcttatta tgcggctgtt tttttcaccg acccgctcat tctggcaaga gctggcatgg
    10201 ttactaagcg cggtgttctg gtgtgcgctg ggggcactgt gtttcttatt tatcagtagt
    10261 ttgtttaaac cacagcacag aaaaaatcag taaagccctc aacgcgaggg cttgtcagac
    10321 gatcaggcgt ccagattttc tttcacccat gcagcaaaat cggtatagcc gccgatatgt
    10381 tgctgatcga caaaaatctg cggcacggtt tctacgggtt tacctgcctt ttgttgtaga
    10441 tcttctttag tgatcccttc cgcacgaata tctacatact gatactgaaa atcatcgcgt
    10501 tcattgctca atttctcagc cagatctttt gcacgcacac agtaagggca acccgaacga
    10561 ccaaaaataa cggtttgcat tatttctctc ctcatagatt tatgcctgta atgatcacgc
    10621 taaaatgtat tcgctgaaag taggtttaac ctgttgcatt aattgctaaa agctataact
    10681 gttaaacaca atacagtgaa aagttttaga ctgaaggctc actttgcaga gggaagcgta
    10741 tgcgcgcgat cggtaaattg cctaaaggcg tgttgatact ggaatttatc ggaatgatgc
    10801 tactggcggt ggcgctgctg tcggtaagcg actccctgtc gctgcctgag ccattttctc
    10861 ggccagaagt gcagattctg atgatttttc tcggtgtttt gctcatgctt cccgctgcgg
    10921 tggtggttat tcttcaggtg gcaaaacgtc ttgccccaca gctgatgaac cgtccaccgc
    10981 aatattcacg ttcagaaaga gaaaaagata atgacgccaa ccattgaact tatttgtggc
    11041 catcgctcca ttcgccattt cactgatgaa cccatttccg aagcgcagcg tgaggcgatt
    11101 attaacagcg cccgtgcgac gtccagttcc agttttttgc agtgcagtag cattattcgc
    11161 attaccgaca aagcgttacg tgaagaactg gtgacgctga ccggcgggca aaaacacgta
    11221 gcgcaagcgg cggagttctg ggtgttctgt gccgacttta accgccattt acagatctgt
    11281 ccggatgctc agctcggcct ggcggaacaa ctgttgctcg gtgtcgttga tacggcaatg
    11341 atggcgcaga atgcattaat cgcagcggaa tcgctgggat tgggcggggt atatatcggc
    11401 ggcctgcgca acaatattaa agcggtgacg aaactgctta aattaccgca gcatgttctg
    11461 ccgctgtttg ggctgtgcct tggctggcct gcggataatc cggatcttaa gccgcgttta
    11521 ccggcctcca ttttggtgca tgaaaacagc tatcaaccgc tggataaagg cgcactggcg
    11581 cagtatgacg agcaactggc ggaatattac ctcacccgtg gcagcaataa tcgccgggat
    11641 acctggagcg atcatatccg ccgaacaatc attaaagaaa gccgcccatt tattctggat
    11701 tatttgcaca aacagggttg ggcgacgcgc taaaaccgcc acgtcgatgt atgatacgcg
    11761 ggcttttgac caggtctgac agagaggtgc agggtgaaaa ttgccatatt gtcccgggat
    11821 ggaacgctct attcgtgtaa gcggctgcgt gaagccgcta tacagcgcgg tcacctggtt
    11881 gaaattcttg atccgctttc ttgctacatg aacataaatc ctgcggcgtc ttctattcac
    11941 tacaaaggcc gcaagttacc ccattttgac gcagtgatcc cgcgtattgg caccgccatt
    12001 accttttatg ggacggcggc actgcgccag ttcgagatgc tggggagcta tccgctcaat
    12061 gagtcggtcg ccattgcccg ggcgcgtgac aaattgcgtt ccatgcaact gctggcgcgt
    12121 cagggcatcg acctgcctgt cacgggcatt gcgcattcgc cggatgatac cagcgattta
    12181 atcgacatgg tcggtggtgc gccgctggtg gtcaagttgg ttgaaggcac gcagggaatt
    12241 ggcgtcgtgc tggcggagac gcgtcaggcg gcggaaagcg tgattgacgc tttccgcggt
    12301 ctgaacgcgc atattctggt gcaggaatat atcaaagagg cgcaagggtg cgatatccgc
    12361 tgtctggttg ttggcgatga agtggtcgct gcgattgaac ggcgggcgaa agagggcgat
    12421 tttcgttcca atttgcatcg tggcggcgcg gcaagcgtcg ccagtatcac accacaggag
    12481 cgtgaaatcg cgataaaagc cgcgcgaacg atggcgctgg acgttgctgg tgtggatatt
    12541 ctgcgtgcta atcgcgggcc gttggtgatg gaggtgaatg cgtcgccggg gctggaagga
    12601 atagaaaaaa ccaccggtat cgacatcgcg ggtaaaatga tccgctggat cgaacgccac
    12661 gctacgaccg aatattgcct gaaaacgggt ggttagtcgc aatcacatta ctgatcatgg
    12721 ttttgcctgc gctttttgcg taagctgtgc cggtcttttt atcgaaagag gttgtacaaa
    12781 attatgacat cgctggtcgt tcctggtctg gatacgctgc gtcaatggct cgatgacctg
    12841 gggatgagtt tttttgaatg tgataactgt caggctctgc atctgcccca tatgcagaat
    12901 ttcgacggtg tctttgatgc caaaatcgat ctgatcgata acacgatcct gttttctgcc
    12961 atggcggaag tccgaccttc agctgtattg ccgctggcgg cggatttatc tgccatcaat
    13021 gccagttcgc tgaccgtgaa agcatttctt gatatgcagg atgataatct gccaaagctg
    13081 gtggtttgcc agtctttatc cgttatgcag ggcgtaacct atgagcagtt tgcatggttc
    13141 gtgcgtcaga gcgaagagca gatttcgatg gtcattcttg aagctaatgc ccatcaactg
    13201 ctgttaccga ctgacgatga agggcaaaac aacgttaccg aaaactattt cctccactga
    13261 taactccttt cgagcacgca gtcgctggtg cagtggctgc gcgctgcaaa attatctgct
    13321 gtttttaacc ttttcttaaa gattatttca cttctcttgt gtcgatttgg ctttatcaca
    13381 tagagcaaat acgcataaaa atttgttaaa taccgttttt taatccgagc tatagtctca
    13441 aaccctggct aaagttattc ttgcgatgct tttatatagc gagcagtgct ggccgggaga
    13501 gagttctctt ttcttacacc ccgccgataa aaaatatgca cgtttattgc atatctttta
    13561 gtgtgacaac ttttgttcgt ttgttaacga actttcagaa ggaaagagat atgaccgcct
    13621 taaataaaaa atggctttcg ggtctggctg cgggtgctct gatggccgtc tctgtcggca
    13681 cgctcgcggc tgaacaaaaa acactccacg tttataactg gtctgattat atcgccccgg
    13741 acacggtggc caatgttgaa aaagaaaccg gtattaaagt cgtctacgat gttttcgact
    13801 ctaacgaagt actggaaggc aaattaatgg cgggtagcac cggctttgac ttggtggttc
    13861 catctgccag ctttcttgaa cgccagttga ctgcgggagt tttccagccg ctggacaaaa
    13921 gcaaattgcc ggagtggaag aatctcgacc cggagctgct gaagctggtc gccaaacacg
    13981 atcccgacaa taaatttgct atgccctata tgtgggcgac gaccgggatt ggctataacg
    14041 ttgataaagt taaagctgtg ctgggcgaaa acgcgcccgt cgatagctgg gacttgatcc
    14101 tcaaacctga aaatctggaa aaactgaaaa gctgcggtgt ctctttcctg gatgcgccag
    14161 aagaagtttt tgctaccgtg ttgaattatc tcggcaaaga tcccaacagc actaaagcgg
    14221 atgattacac cggaccggca accgatctgc tgttaaagct gcgcccgaac attcgttatt
    14281 tccattcatc tcaatacatt aacgacctgg caaacggcga tatttgcgtc gctatcggct
    14341 gggcaggtga tgtctggcag gcgtcaaacc gcgcgaagga agcgaagaat ggagtgaatg
    14401 tctcgttctc gattccaaaa gaaggggcga tggcgttctt tgatgtattc gccatgcctg
    14461 cggatgccaa aaacaaagac gaagcctatc agttcctgaa ttacctgctg cgcccggatg
    14521 tagtagcgca tatttccgac catgtgttct atgccaacgc caataaagca gccacgccgc
    14581 tggtgagtgc ggaagtccgt gagaacccgg gtatttatcc gcctgcggat gttcgtgcga
    14641 agctgttcac tctgaaagtg caggacccga aaatcgaccg tgtgcgcacc cgcgcgtgga
    14701 ccaaagtgaa gagcggaaaa taatccgcag ccttagatgc cgggcgggcg caccacaccc
    14761 gccggcaatt cgcaccatca tggtgcgctt gcacacattc aatgccggag agcagccgtg
    14821 aatgacgcta tccctcgccc gcaggcgaaa acccgtaagg cgctgacgcc gctattagaa
    14881 atccgcaacc tcaccaaatc ctacgatggt caacatgcgg tggatgatgt cagcctgacc
    14941 atctacaaag gtgaaatctt cgcgctgctg ggcgcatccg gctgtggcaa gtccacgctg
    15001 ctgcgtatgc tggcaggttt cgaacaacct tctgccggac agataatgct tgatggcgtc
    15061 gatttgtcac aggtgccgcc ttacctgcgc cccatcaata tgatgtttca gtcttacgcg
    15121 ctgtttccgc atatgaccgt ggaacagaac atcgcttttg gcctgaaaca ggacaaacta
    15181 ccgaaagcgg aaattgccag tcgggtccat gagatgctcg gcctggtgca catgcaggag
    15241 ttcgccaaac gcaaacctca tcagctttcc ggtggtcagc gacagcgtgt ggccctggcc
    15301 cgaagccttg ccaaacgccc gaaactatta ctgctcgatg agccgatggg cgcgctggat
    15361 aaaaagctgc gtgaccggat gcagcttgaa gtggtggata ttctggagcg cgtcggtgtg
    15421 acttgtgtga tggtcaccca cgatcaggaa gaggcgatga ccatggcggg gcgtatcgcc
    15481 attatgaatc gcgggaaatt tgtccagatt ggcgagccgg aagagatcta cgagcatcca
    15541 actacccgct acagcgctga atttatcggt tcggttaatg tctttgaagg tgtactcaaa
    15601 gagcgtcagg aagatggcct ggtgcttgat tcgccggggc tggtgcatcc actgaaagtc
    15661 gatgcggatg cttcagtggt cgataacgtg ccggtacacg tagcgctgcg cccggaaaaa
    15721 atcatgcttt gcgaagagcc gcccgccaat ggttgtaact tcgcggtggg ggtggtgata
    15781 cacattgcct atctcggcga tctttcggtg tatcacgttc gtctgaaaag tgggcagatg
    15841 attagcgccc agctacaaaa cgcccatcgt catcgtaaag ggttaccgac ctggggcgac
    15901 gaagtgcgtt tgtgctggga agtggacagc tgtgtggtgc tgacggttta aggagcaaag
    15961 atgaatacac ttgaacctgc tgcccagtcg aaaccgccgg gcggatttaa gctgtggttg
    16021 tcgcagctgc aaatgaagca tgggcgcaaa ctggtcattg cgttgccata tatctggttg
    16081 atcttgctgt ttctgctgcc atttctgatt gtctttaaaa taagcctggc ggagatggcg
    16141 cgcgctattc caccttatac cgaactgatg gagtgggctg acgggcaact gtccatcact
    16201 cttaatctcg gtaattttct gcaactgacc gacgatccgc tctatttcga tgcttatctc
    16261 cagtcgctac aggtggctgc gatttcgaca ttttgctgtt tactgatcgg ctatccgctg
    16321 gcgtgggcgg tggcgcacag taagccttcg acccgtaata ttttattact actggtgatc
    16381 ctgccgtcgt ggacctcgtt tctgatccgc gtttatgcct ggatgggaat attaaaaaac
    16441 aacggtgtgc tgaataattt tctgctgtgg ctgggggtta tcgatcaacc gctgaccatt
    16501 cttcatacca atctggcagt ttatattggc attgtttacg cctacgtgcc gtttatggta
    16561 ctgccgattt ataccgcgtt gattcgtatt gattattcgc tggtggaagc agcgctggat
    16621 ctcggtgcac gaccgctgaa aacgttcttt actgtgatcg tgccgctgac taaaggtggg
    16681 attattgccg gatcgatgct ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa
    16741 ctgctcggtg gcccggacag catcatgatc gggcgcgtgc tatggcaaga gtttttcaat
    16801 aaccgcgact ggccggtggc ctcggcggta gcgatcatca tgttgctgct gctaattgtg
    16861 ccgataatgt ggttccacaa acaccagcaa aaaagcgtgg gagaacacgg atgaataatt
    16921 taccggtagt tcgttcgccc tggcggattg tgattttgct gctgggcttt acctttctct
    16981 acgcgccaat gctgatgctg gttatctatt cgtttaacag ctcgaagctg gtgacggtgt
    17041 gggccggctg gtcaacgcgc tggtatggtg agttattgcg cgatgacgcg atgatgagtg
    17101 cggttggttt aagcctgaca attgctgcct gtgcggcaac ggcggcggcg atcctcggga
    17161 ctattgcggc ggtggtgctg gtgcgttttg gcaggtttcg cggatcaaat ggttttgcct
    17221 ttatgatcac cgcgccgctg gtgatgccag atgtcatcac gggcttgtcg ctgttgttat
    17281 tattcgtcgc gcttgctcat gccattggct ggcctgcgga ccgcggtatg cttaccatct
    17341 ggctggcgca tgtcactttc tgtacggctt atgtggcagt cgttatttcg tcgcgtctgc
    17401 gggaactgga tcgctcgata gaagaagcag cgatggatct cggtgcgacg ccgctgaaag
    17461 tgtttttcgt cattacgcta ccgatgatca tgcccgcgat catttctggc tggttactgg
    17521 cttttacttt gtcgcttgat gatctggtga tcgccagctt tgtttccggg ccgggagcca
    17581 ccacgttacc gatgctggtc ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg
    17641 ccctggcaac gttaattctc ggtgcggtcg gaattgtcgg atttatcgcc tggtatctga
    17701 tggctcgcgc agaaaaacag cggatacgcg atatccagcg tgcaagacgt ggctgaagac
    17761 actaaaattt gccaacctgg ctacataatg ccgcgcatgt cgcggcattg ttttcatgga
    17821 agacgaaacg ttgggatttt ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc
    17881 tgcgctggtg caggtggcgg cgctcgccat tattatgatc cgtggcctcg acgtgctgat
    17941 gattttcaat acgctgggcg tgcgcggtat tggcgagttc attcatcgca gcgtacaaac
    18001 ctggagttta acgctggtct ttttaagcag tctggtgctg gttttcattg agatctggtg
    18061 tgcgttttca ctggtgaaag ggcgtcgctg ggcgcgctgg ctatatctgc tgacacaaat
    18121 caccgccgca agttacttgt gggcggcttc gctggggtat ggttatccgg agctgttcag
    18181 cattcccggt gaatcaaaac gtgaaatctt ccatagcctg atgctgcaga agctgccgga
    18241 tatgctcatc ctgatgctgc tgttcgttcc ctcgaccagt cggcggttct tccagttgca
    18301 ataatgtgta taatcgtcgc ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt
    18361 acgacgcggg tcgctgtcgt tcctgtcagt ggataacgca gccgattcca gagcaactct
    18421 ccgctaaaac cgccgatctt aaaaatctgc tcgccgattt tccggttgag gaatggtgcg
    18481 cgccggtgtc aggcccggaa caagggtttc gtaataaagc caaaatggtg gtgagtggta
    18541 gcgttgaaaa accactgctc ggtatgctgc atcgcgatgg cacgccggaa gacctttgtg
    18601 actgcccgct ttatcctgcc tcttttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg
    18661 cccgtgcggg gttaacgccc tacaacgtgg cgcgtaagcg tggcgaactg aaatacattc
    18721 tgctgactga aagccagagc gatggcggca tgatgctgcg ctttgtactg cgttctgaaa
    18781 ccaaactggc acaactgcgt aaggcgctgc cgtggttaca ggaacaacta ccgcaactga
    18841 aagttattac cgtcaatatt cagccggtac atatggcgat tatggaaggg gagacggaga
    18901 tctacctgac tgaacaacag gcgctggcag agcgttttaa tgacgtaccg ctgtggatcc
    18961 gtccgcaaag tttcttccag actaatccgg cggtcgccag ccagctgtac gctaccgcgc
    19021 gcgactgggt acggcagctg ccagttacac atatgtggga tctgttctgc ggtgtgggtg
    19081 gctttggttt acactgcgcg acgcctgaca tgcagttaac cgggatcgaa attgcatcag
    19141 aggccattgc ctgtgcaaag cagtcagccg ctgaactggg cttaacgcgt ttgcaatttc
    19201 aggcgctgga ctccactcag tttgccaccg ctcaggggga tgtgccggag ctggtgctgg
    19261 ttaacccgcc gcgccgcggc attggtaaac cgctgtgtga ttatctctca acgatggcac
    19321 cgcgttttat tatctactcc agctgtaacg cccaaaccat ggcgaaagat atccgcgaac
    19381 tgccaggtta ccgcatcgaa cgggtacagc tttttgatat gttcccgcac accgcgcact
    19441 atgaagtgat gacgctgctg gtgaagcaat aaaaaagccg caggtgcggc ttcagattgc
    19501 tgatcaggtg cgctttgttc atgccggatg cggcgtgtac gccttatccg gcctacaaaa
    19561 tcatgctaaa tcaatacatt gcagagttga tgtgggccag ataagcgcag cgcatcaggc
    19621 aatcttactt ttgtcatcag tctcaattac tgcgggaacc actggtcatt gattttctga
    19681 tatgtaccgt cagctttaat tgctgccagc gcgttattca gtttttccag cagagctttg
    19741 ttatccggac gtacagcgat gcccagaccg gtgccgaagt attgcggatc ggtcactttt
    19801 tcagtagcaa cgcccagttg cggattggtt ttcagccatt cgtttaccac cgctgtgtca
    19861 ccaaataccc catcaatacg accatttttc agatcgataa aggcattctg ataactgtca
    19921 taggagacag ttttcacttc ggggtgctga tcctgaatat atttctggtg cgtggtgccg
    19981 ttttccatcc caatacgttt gcctttcaga tcggcaaacg ttttgtaggt atcttttttg
    20041 gcaatcacga cggctgagtt ttcatagtag ggcgtggtaa acgatacctg cttgctacgc
    20101 tcgggcgtga tatccatacc ggagattacg gcgtcatatt ttctgaattt cagtgacggg
    20161 atcaggctgt cgaacgcgtg attagtaaaa gtacattctg cctgcatttg tttgcacaag
    20221 gcttttgcca gatcgatatc aaagccgaca atctcattat tagcacctat agattcaaag
    20281 gggggatagg tggctgaaac gccaaaattg attttctctg cggcagaagc accgaaagta
    20341 aaggaagcaa gtaaagcggc aagaactaac tttttcatga tggaactccc gtctgtcaat
    20401 cttatgattt ttggccgtgt ctgcggcatg ggataacaat gccatcaagt gaatttata
//
LOCUS       DAZKIV010000081        20228 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZKIV010000081 DAZKIV010000000
VERSION     DAZKIV010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.081"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..889
                     /gene="yieE"
                     /locus_tag="ACHOT6_002838"
     CDS             8..889
                     /gene="yieE"
                     /locus_tag="ACHOT6_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFP9948024.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIKDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1094..2563
                     /gene="lysP"
                     /locus_tag="ACHOT6_002839"
     CDS             1094..2563
                     /gene="lysP"
                     /locus_tag="ACHOT6_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFP9948025.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2855..4834
                     /gene="cirA"
                     /locus_tag="ACHOT6_002840"
     CDS             2855..4834
                     /gene="cirA"
                     /locus_tag="ACHOT6_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFP9948026.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWREGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFTA
                     NGVPVFSYYNVNKARIQGVETELKIPFNDEWKLSLNYTYNDGRDVSNGENKPLSDLPF
                     HTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVTKDVK
                     LRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4866..5702)
                     /gene="yeiG"
                     /locus_tag="ACHOT6_002841"
     CDS             complement(4866..5702)
                     /gene="yeiG"
                     /locus_tag="ACHOT6_002841"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFP9948027.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVASELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5960..6628
                     /gene="folE"
                     /locus_tag="ACHOT6_002842"
     CDS             5960..6628
                     /gene="folE"
                     /locus_tag="ACHOT6_002842"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFP9948028.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6645..7802
                     /gene="yeiB"
                     /locus_tag="ACHOT6_002843"
     CDS             6645..7802
                     /gene="yeiB"
                     /locus_tag="ACHOT6_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFP9948029.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVIINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMQFDR
                     LELLVFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7944..8984
                     /gene="galS"
                     /locus_tag="ACHOT6_002844"
     CDS             7944..8984
                     /gene="galS"
                     /locus_tag="ACHOT6_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFP9948030.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYTHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9264..10262
                     /gene="mglB"
                     /locus_tag="ACHOT6_002845"
     CDS             9264..10262
                     /gene="mglB"
                     /locus_tag="ACHOT6_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFP9948031.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10323..11843
                     /gene="mglA"
                     /locus_tag="ACHOT6_002846"
     CDS             10323..11843
                     /gene="mglA"
                     /locus_tag="ACHOT6_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFP9948032.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11859..12869
                     /gene="mglC"
                     /locus_tag="ACHOT6_002847"
     CDS             11859..12869
                     /gene="mglC"
                     /locus_tag="ACHOT6_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFP9948033.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(13112..14347)
                     /gene="preA"
                     /locus_tag="ACHOT6_002848"
     CDS             complement(13112..14347)
                     /gene="preA"
                     /locus_tag="ACHOT6_002848"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFP9948034.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14341..15579)
                     /gene="preT"
                     /locus_tag="ACHOT6_002849"
     CDS             complement(14341..15579)
                     /gene="preT"
                     /locus_tag="ACHOT6_002849"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFP9948035.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLSLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15773..16012)
                     /gene="yeiS"
                     /locus_tag="ACHOT6_002850"
     CDS             complement(15773..16012)
                     /gene="yeiS"
                     /locus_tag="ACHOT6_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFP9948036.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(16015..16734)
                     /gene="sanA"
                     /locus_tag="ACHOT6_002851"
     CDS             complement(16015..16734)
                     /gene="sanA"
                     /locus_tag="ACHOT6_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFP9948037.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDLSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16884..17768)
                     /gene="cdd"
                     /locus_tag="ACHOT6_002852"
     CDS             complement(16884..17768)
                     /gene="cdd"
                     /locus_tag="ACHOT6_002852"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFP9948038.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17898..18593)
                     /gene="yohK"
                     /locus_tag="ACHOT6_002853"
     CDS             complement(17898..18593)
                     /gene="yohK"
                     /locus_tag="ACHOT6_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFP9948039.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18590..18988)
                     /gene="yohJ"
                     /locus_tag="ACHOT6_002854"
     CDS             complement(18590..18988)
                     /gene="yohJ"
                     /locus_tag="ACHOT6_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFP9948040.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAI
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19227..20177
                     /gene="dusC"
                     /locus_tag="ACHOT6_002855"
     CDS             19227..20177
                     /gene="dusC"
                     /locus_tag="ACHOT6_002855"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFP9948041.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         4959 a         4998 c         5284 g         4987 t
ORIGIN      
        1 agcgactatg cacatcaccc tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg
       61 atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt
      121 gaccgacctg gaagggcagc ttggcgttca actgtttgat cgcgtgggga aaagactggt
      181 tgttaatgaa cacgggcggc tgctctatcc gcgagcgttg gcactgcttg aacaggcggt
      241 tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac
      301 catcggtaac tacattctgc ctgcagttat cgctcgttat cgccatgatt atccgcagtt
      361 gccgattgaa cttagcgttg ggaacagcca ggacgtgatg caagcggtgc tggatttccg
      421 cgttgatatt ggttttattg aaggaccgtg ccacagcact gaaattattt ctgaaccgtg
      481 gctggaagac gagctggtgg ttttcgccgc gccgacttcg ccgttggccc gtggtccggt
      541 caccttagag cagctggccg ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg
      601 ggagattgtc gattatctat tgctgtcaca tttaccgaag tttgagatgg cgatggaatt
      661 aggtaactcc gaggcaatca aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc
      721 gcgacgggtg attaaagatc aattgcaggc aggcacatta agtgaagttg cggtccctct
      781 gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg caaaaacacc tttccaacgc
      841 gctacggcgc tttctggact attgcgatcc cgcgaatgta ccgcgttaag ttgctgtaca
      901 agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc ctcagcatgc attcgccaga
      961 aaagagattg gctgctttac ttataatccc tgggcgatca tgaaggtgtc ttataaccgt
     1021 gtatttctgc cggaaggatt gccaatcgtc tgctacaatc gcgcctcatt tttaagatgg
     1081 atagcatttt tgtatggttt ccgaaactaa aaccacagaa gcgccgggct tacgccgtga
     1141 attaaaggcg cgtcacctga cgatgattgc cattggcggt tccatcggta caggtctttt
     1201 tgttgcctct ggcgcaacga tttctcaggc aggtccgggc ggggcattgc tctcgtatat
     1261 gctgattggc ctgatggttt acttcctgat gaccagtctc ggtgaactgg ctgcatatat
     1321 gccggtttcc ggttcgtttg ccacttacgg tcagaactat gttgaagaag gctttggctt
     1381 cgcgctgggc tggaactact ggtacaactg ggcggtgact atcgccgttg acctggttgc
     1441 agctcagctg gtcatgagct ggtggttccc ggatacaccg ggctggatct ggagtgcgtt
     1501 gttcctcggc gttatcttcc tgctgaacta catctcagtt cgtggctttg gtgaagcgga
     1561 atactggttc tcactgatca aagtcacgac agttattgtc tttatcatcg ttggcgtgct
     1621 gatgattatc ggtatcttca aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg
     1681 cgaagcgccg tttgctggtg gttttgcggc gatgatcggc gtagctatga ttgtcggctt
     1741 ctctttccag ggaaccgagc tgatcggtat tgctgcaggc gagtccgaag atccggcgaa
     1801 aaacattcca cgcgcggtac gtcaggtatt ctggcgaatc ctgttgttct atgtgttcgc
     1861 gatcctgatt atcagcctga ttattccgta taccgatccg agcctgctgc gtaacgatgt
     1921 taaagacatc agcgttagtc cgttcaccct ggtgttccag cacgcgggtc tgctctctgc
     1981 ggcggcggtg atgaacgcag ttattctgac ggcggtgctg tcagcgggta actccggtat
     2041 gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac ggtaaagcgc cgcgcatttt
     2101 cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc
     2161 cggtctgtgc ttcctgacct ccatgtttgg caaccagacg gtatacctgt ggctgctgaa
     2221 cacctccggg atgacgggtt ttatcgcctg gctggggatt gccattagcc actatcgctt
     2281 ccgtcgcggt tacgtattgc agggacacga cattaacgat ctgccgtacc gttcaggttt
     2341 cttcccactg gggccgatct tcgcattcat tctgtgtctg attatcactt tgggccagaa
     2401 ctacgaagcg ttcctgaaag ataccattga ctggggcggc gtagcggcaa cgtatattgg
     2461 tatcccgctg ttcctgatta tttggttcgg ctacaagctg attaaaggaa ctcacttcgt
     2521 acgctacagc gaaatgaagt tcccgcagaa cgataagaaa taagtttcct cccttccttg
     2581 ctaagccctc tcaaccgaga gggctttttc aattccattt ccctgacaaa tcatgcggat
     2641 ataaaattta acatttggat tgataattgt tatcgtttgc attatcgtta cgccacaatc
     2701 aaaaaaggct gacaaatcag aggctggtcc ggctttctgg gatgatcacc tgcatacaaa
     2761 taagtccacc gcgatgctgc cgtacgcaag gggacgtaaa gaagatgtga gcgataccca
     2821 ttttattttc gtagttacct catggagata tggaatgttt aggttgaacc ctttcgtacg
     2881 ggtcgggctg tgtttgtccg ctatttcttg tgcatggcct gtgttagcgg tcgatgacga
     2941 tggcgaaacg atggttgtca ctgcatcttc cgttgaacaa aaccttaaag atgcacccgc
     3001 cagtatcagc gtcattaccc aggaagatct gcagcgaaaa ccggtacaga atctgaagga
     3061 tgtcctcaaa gaagtgcctg gcgtacaact gacgaacgaa ggggataacc gtaagggcgt
     3121 tagtattcgt ggtctggaca gcagctacac cctgattctt gtcgacggta aacgcgttaa
     3181 ctcccgcaat gccgtcttcc gccacaatga tttcgatctg aactggatcc cggtcgattc
     3241 catcgaacgt attgaagtgg tccgtggccc gatgtcgtcg ctgtacggtt ccgatgcgct
     3301 cggcggtgta gtgaatatca tcaccaaaaa aatcggtcag aaatggtcgg gtaccgttac
     3361 cgtcgatacc accattcagg aacatcgcga tcgcggtgat acctataacg gtcagttctt
     3421 taccagtgga ccattaattg atggtgtgct gggaatgaaa gcttacggca gcctggcaaa
     3481 acgtgaaaag gatgacccgc aaaactcaac gaccaccgat accggagaaa cgccgcgtat
     3541 tgaaggattc tccagccgcg acggcaatgt cgaatttgcc tggacaccga atcaaaatca
     3601 cgattttact gccggatacg gtttcgaccg tcaggatcgt gattccgact cgctggacaa
     3661 aaaccgcctg gaacgccaga actactccgt cagccataat gggcgttggg attacggcac
     3721 cagcgaactg aaatactacg gtgagaaagt cgagaacaaa aaccctggca acagcagccc
     3781 gataacttcc gaaagcaata cggtcgacgg caaatacacg ttgccgctga cggcgattaa
     3841 tcagtttctc acggttggcg gtgaatggcg tcacgacaaa cttagcgatg cggtgaacct
     3901 gaccggggga accagctcca aaacgtctgc cagccagtac gcgctgtttg tggaagatga
     3961 atggcggatc ttcgagccgc tggcgctgac gaccggcgtg cgtatggacg atcacgaaac
     4021 ctacggtgaa cactggagtc cgcgtgccta cctggtttat aacgccaccg acaccgtaac
     4081 ggtgaaaggg ggctgggcga cggcatttaa agcaccttct ctgttgcaac ttagccctga
     4141 ctggacgagc aattcctgcc gtggcgcatg taagattgtg ggtagcccgg atctgaaacc
     4201 agaaaccagc gaaagttggg agctggggct ttactacatg ggtgaagaag gctggcggga
     4261 aggggttgaa tccagcgtta ccgttttccg taacgatgtg aaagatcgta tcagcatcag
     4321 ccgtacgtct gacgtcaatg ctgcaccggg ctaccaaaac tttgttggtt ttacagcgaa
     4381 cggtgtgccg gtatttagct actacaacgt taacaaagca cgtattcagg gcgtggaaac
     4441 cgagctgaaa attccgttca acgatgaatg gaaactgtca ctcaactaca cctacaacga
     4501 tggtcgtgat gtcagcaacg gcgaaaacaa accgctatcc gatctgccgt tccataccgc
     4561 taacggtacg ctggactgga aaccgctggc gctggaagac tggtcattct atgtttctgg
     4621 tcactatacc gggcagaaac gagccgacag cgcgacggct aaaacaccgg gcggttatac
     4681 catctggaat accggcgcgg cctggcaggt gactaaagac gtcaaactgc gcgcaggcgt
     4741 gctgaacctt ggcgacaagg atctcagtcg tgacgactac agctataacg aagacggacg
     4801 tcgttacttt atggcagtgg attatcgctt ctgatgagaa gatgcccggc gaaccgggcg
     4861 gactttcact tcagtaaata ctgcgcatgg aagcgcaggt gatcctctat aaaagaggcg
     4921 atgaagtagt aactgtgatc atatcccggc tgaatacgca gcgtcatcgg ccatgctttc
     4981 tggcgcgcgg cttctgcaag tactgcaggt tgcagttggt cagcaagaaa ctgatcgtta
     5041 tcaccctgat cgataagcgt cgggatcgca tcctgcgcgt tactggcata catcagcgcg
     5101 caactgtccc attccagcca tgcatttttg tcctcaccta aatagcggct aaacgctttg
     5161 attccccacg ggacgctgca cggattcaca attggcgcaa aggccgaaac gctggtgtat
     5221 ttgcccgggt ttttcagcgc cataatcagc gcaccgtgac cacccattga atgaccgcta
     5281 atagcgcagc ggtcgctgac attaaattgc gactgaacca gcgccggtaa ttcatcacgc
     5341 agataatcat acatccggta atgcgtcgcc cacggcggtt gcgtggcatt aagataaaag
     5401 cctgcgccct ggcctaaatc gtagccatcg tcgttggcaa ccttttcgcc gcgcgggctg
     5461 gtgtctggca taaccagtac aatccccagt tccgacgcca cgcgctgggc acccgccttg
     5521 gtggtgaagt tctcgtcatt gcaggttaat ccggaaagcc agtacagcac tggtggcggg
     5581 gtgtgatcac gagggggtgg gagaaagata ctgaacgtca tcgggcagtt taaggtactg
     5641 gagtcgtgtc gccagcgttg ctgccagcct tcaaaacagc ggtgctcttc gagcatttcc
     5701 atgcgtggct ccttgttgtg ttgtttgcaa tttcttcatc ataccgttta ttcaatgtgc
     5761 tgtgagtaac tttcacttcc gtatttgcat aacgatgttt taacatctgc tgatgaaagg
     5821 cagcggcaat tacaataatt atcgctgtga atactggatt atgtgcgccg cctcacgcac
     5881 aataatcagg ctgtaaatca gcttaataac tttgccccca cgcagggcgg aggcgtcaca
     5941 cctgcaggag aaatcataaa tgccatcact cagtaaagaa gcggccctgg ttcatgaagc
     6001 gttagttgcg cgaggactgg aaacaccgct gcgcccgccc gtgcatgaaa tggataacga
     6061 aacgcgcaaa agccttattg ctggtcatat gaccgaaatc atgcagctgc tgaatctcga
     6121 cctggctgat gacagtttga tggaaacgcc gcatcgcatc gctaaaatgt atgtcgatga
     6181 aattttctcc ggtctggatt acgccaactt cccgaaaatc accctcattg aaaacaaaat
     6241 gaaggtcgat gaaatggtca ccgtgcgcga tatcactctg accagtacct gtgaacacca
     6301 ttttgttacc atcgatggca aagcgacggt ggcttatatc ccgaaagatt cggtgatcgg
     6361 tctgtcaaaa attaaccgta ttgtgcagtt ctttgcccag cgtccgcagg tgcaggaacg
     6421 tctgacgcag caaattctca ttgcgctaca aacgctgctg ggcaccaata acgtggctgt
     6481 ctctatcgac gcggtgcatt attgcgtgaa ggcgcgtggc atccgcgatg caaccagtgc
     6541 cacgacaacg acctctcttg gtggattgtt caaatccagt cagaatacgc gccacgagtt
     6601 tctgcgcgct gtgcgtcatc acaactgatt taaaggcagg aaccatggag cgcaacgtca
     6661 cgctcgactt tgttcgcggc gtcgccattc tggggatcct gctattaaac atcagcgcct
     6721 ttgggctacc aaaggcggct tatcttaatc ccgcctggta cggcgctatt acgccgcagg
     6781 atgcatggac ctgggcattt ctcgatctca tcggccaggt gaaattcctc acgctttttg
     6841 cgctgctgtt tggtgctggc ctgcaaatgt tgctgccccg tggcagacgc tggatccagt
     6901 cgcggttaac gctgttagtc ttgctgggct ttattcacgg tttattgttc tgggacggcg
     6961 atattctgct ggcttacggg ctggtgggct taatctgctg gcggctggtg cgcgatgcgc
     7021 catcggtaaa aagccttttt aataccggcg tcatgcttta tctggtgggg cttggcgttt
     7081 tgctgttatt ggggttgatt tccgatagcc agactagccg cgcctggacg ccggatgcat
     7141 cggctatttt atatgaaaaa tactggaagc ttcacggcgg cgttgaagcg atcagtaatc
     7201 gtgccgatgg tgttgggaac agtttattgg cactgggcgc acagtatggc tggcaactgg
     7261 ctgggatgat gctcattggt gccgcattga tgcgcagtgg ctggctgaaa gggcagttca
     7321 gcttacgtca ctatcgtcgt actggttttg tgctggtggc gattggggtg atcattaacc
     7381 ttcctgccat cgccctgcaa tggcagctgg actgggcgta tcgctggtgt gccttcttac
     7441 ttcaaatgcc gcgggaactg agtgcgccgt ttcaggcgat tggctatgcg tcgctgtttt
     7501 atggcttctg gccgcaattg agccgcttta agctggtgct ggcgatcgcc tgcgtcggac
     7561 ggatggcgct gaccaactat ctattgcaaa cgctgatttg taccacgctt ttttaccacc
     7621 tcggtctgtt tatgcagttt gaccgtctgg aattgctggt gtttgttatt ccggtatggc
     7681 tggcgaatat tctcttctct gttatctggc tgcgttactt ccgccagggg ccggtggaat
     7741 ggctctggcg tcagttaact ttgcgtgctg ccggaccggc aatatctaaa acatcaagat
     7801 aacgatctgg atcacattcg ttaacaaaac ggctgtaacc gtttccatcg ctgtgactcg
     7861 attcacgaag tcctgtattc agtgctgaca aaatagccgg cagcaagcag tcatttactg
     7921 caatctcata acaggtagtg aatatgatca ccattcgtga tgtagcgcgt caggctggcg
     7981 tctctgtggc aacggtttcc cgggtgctca ataacagcac gctggtcagt gccgacacgc
     8041 gtgaagcagt aatgaaagcc gtgagtgagc tggattatcg gccaaacgcc aatgcccagg
     8101 cgctggcaac tcaggttagc gacaccattg gcgtggtggt gatggacgtt tctgatgcgt
     8161 ttttcggcgc gctggtaaaa gcggtggatc tggtcgctca gcagcatcag aaatacgtgc
     8221 taatcggcaa tagctatcat gaagcggaaa aagagcgtca cgccattgag gtgttaattc
     8281 gccagcgttg taatgcgttg attgttcact caaaagcatt gagtgacgat gaactggcgc
     8341 aatttatgga taacattccc ggtatggtgt taatcaaccg cgttgtgccg gggtacaccc
     8401 atcgttgcgt ttgcctggat aatctcagcg gtgcccgaat ggcgacgcgc atgttgctga
     8461 ataacggtca tcaacgtatt ggttatcttt cttccagcca cggcattgaa gatgacgcca
     8521 tgcgtaaagc aggctggatg agtgcgttga aagagcagga tattattccg ccggaaagct
     8581 ggattggcac tggtacgccg gacatgccgg gcggtgaggc ggcgatggtt gaactgctgg
     8641 ggcgcaatct acaacttacc gctgtatttg cttataacga caatatggct gctggcgcac
     8701 tgacagcatt aaaagataat ggcattgcga ttccgttaca tctctcaatc atcggtttcg
     8761 atgatattcc catcgcccgt tacaccgacc cgcaattaac gaccgtgcgt tatcccattg
     8821 cttcaatggc gaaattagcc accgaactgg ccttgcaggg ggcagcaggc aatattgatc
     8881 ctcgtgccag ccactgtttt atgccgacgt tagtgcgtcg ccattctgtc gcaacacgcc
     8941 agaatgcggc ggcgatcact aactcaacaa atcaggcgat gtaaccgctt tcaatctgtg
     9001 agtgatttca cagtatctta acaatgtgat agctatgatt gcaccgtttt aacgttgtaa
     9061 cccgtatgta acagtgaata atcactttca ccgaggtaat agcgtcataa caacaattaa
     9121 agccgttttc tggagcgtta ccgggcatgg aagaacgaat tttaaaaagt gagcttcggc
     9181 gttcagtaac acttcattaa ctctactgcc ccgccgagca tttatctcaa gcactaccct
     9241 gcataagaaa aaccggagat accatgaata agaaggtgtt aaccctgtct gctgtgatgg
     9301 ccagcatgtt attcggtgcc gctgcacacg ctgctgatac tcgcattggt gtaacaatct
     9361 ataagtacga cgataacttt atgtctgtag tgcgcaaggc tattgagcaa gatgcgaaag
     9421 ccgcgccaga tgttcagctg ctgatgaatg attcacagaa tgaccagtcc aagcagaacg
     9481 atcagatcga cgtattgctg gcgaaagggg tgaaggcact ggcaatcaac ctggttgacc
     9541 cggcagctgc gggtacggtg attgagaaag cgcgtgggca aaatgtgccg gtggtattct
     9601 ttaacaaaga accgtctcgt aaggcgctgg atagctacga caaagcctac tacgttggca
     9661 ctgattccaa agagtccggc attattcagg gcgatttgat tgctaaacac tgggcggcga
     9721 atcagggttg ggatctgaac aaagacggtc agattcagtt cgtactgctg aaaggtgaac
     9781 cgggccatcc ggatgcagaa gcacgtacca cttacgtgat taaagaattg aacgataaag
     9841 gcatcaaaac tgaacagtta cagttagata ccgctatgtg ggataccgct caggcgaaag
     9901 ataagatgga cgcctggctg tctggcccga acgccaacaa aatcgaagtg gttatcgcca
     9961 acaacgatgc gatggcaatg ggcgcggtag aagcactgaa agcacacaac aagtccagca
    10021 ttccggtgtt tggcgtcgat gcgctgccag aagcgctggc gctggtgaaa tccggtgcac
    10081 tggcgggcac agtactgaac gatgctaaca accaggcgaa agcgaccttt gatctggcga
    10141 aaaacctggc cgatggcaaa ggtgcggctg atggcaccaa ctggaaaatc gacaacaaag
    10201 tggtccgcgt accttatgtt ggcgtagata aagacaacct ggctgaattc agcaagaaat
    10261 aagactgatt gggcgcagtc gtttactgcg cccttattgt atggccaaca aggtaaaatt
    10321 atatggtcag ctcaacgact ccgtcttccg gggaatactt gttggaaatg agcggtatca
    10381 acaagtcctt tcctggtgtt aaggcacttg ataacgttaa tttaaaagtc cggccacatt
    10441 ctatccatgc attaatgggg gaaaacggtg caggaaaatc gacattatta aaatgcctgt
    10501 ttggtattta tcaaaaagac tccggcacca ttttattcca gggtaaagag atcgatttcc
    10561 attccgccaa agaggcactg gaaaatggta tttcgatggt acaccaggag ttaaacctgg
    10621 tattacaacg ttcggtgatg gataacatgt ggctggggcg atatcccacc aaaggcatgt
    10681 ttgtcgatca ggacaaaatg taccgcgaaa ccaaagcgat ttttgatgaa ctggatattg
    10741 atatcgatcc gcgtgcgcgc gtcggcacat tatccgtttc gcaaatgcag atgatcgaaa
    10801 tcgccaaagc gttttcctat aacgcgaaaa ttgtgattat ggatgaaccg acttcttcgt
    10861 taaccgaaaa agaggtcaat catctgttca ctattattcg taaattaaaa gagcgcggct
    10921 gcggtattgt ttatatctcg cataaaatgg aagaaatctt ccagttatgt gatgaagtta
    10981 ccgtattgcg cgacggtcag tggatcgcca ccgaaccgct ggcaggactg acgatggaca
    11041 agatcatcgc catgatggtt gggcgttctc ttaatcagcg ttttcctgac aaagaaaaca
    11101 agccgggcga agtcatcctc gaggtgcgta acctaacgtc actgcgccag ccgtcgattc
    11161 gcgatgtctc gtttgatctg cataaagggg agatcctcgg tattgccggg ctggtggggg
    11221 cgaaacgtac cgatattgtt gagacgttat ttggtattcg cgagaaatcg gctggcacca
    11281 ttacgttgca cggcaaacag atcaataacc ataatgccaa cgaagccata aaccacggat
    11341 ttgcactggt aactgaggag cgccgctcaa cgggaattta tgcctatctg gatattggtt
    11401 ttaactcgtt aatttccaat attcgcaact acaaaaataa agttggttta ctggataact
    11461 cgcggatgaa aagcgatacc cagtgggtga ttgattcgat gcgggtaaaa acgccgggtc
    11521 atcggacgca aattggttcg ctctccggtg gtaatcagca aaaggtgatt attggtcgct
    11581 ggctactaac gcaaccagaa atattaatgc tcgatgaacc gacgcgcggt attgatgtcg
    11641 gggcgaagtt tgaaatttat cagttaattg ccgaactggc gaagaaaggc aaggggatta
    11701 ttattatctc ctctgaaatg cctgagttgt tagggataac agaccgtatt ctggtcatga
    11761 gcaatggtct cgtttccgga attgtcgata caaaaacaac aacgcaaaac gaaattctgc
    11821 gtcttgcgtc tttgcacctt taagattagg ggcttcccat gagtgcgtta aataagaaaa
    11881 gttttcttac ttacctgaaa gagggcggta tttacgtcgt tcttttagtt ttgctggcga
    11941 ttattatttt ccaggaccca acatttttaa gtctgttgaa cttaagtaat attctcaccc
    12001 agtcatcggt gcgtattatt atcgcgctcg gtgtggcagg gttaattgtc acccagggga
    12061 ccgatctttc tgctggtcgt caggtagggc tggcggcagt ggtggctgcg acattattgc
    12121 agtccatgga taacgccaac aaagtgttcc cggaaatggc gacgatgccg attgcgctgg
    12181 ttattctgat tgtctgtgcc attggtgcgg tgatcggttt gatcaacggt ctgattatcg
    12241 cttatctcaa cgtgacgccg ttcattacca cgctcggcac gatgatcatc gtctatggaa
    12301 tcaactcgct ctattacgac tttgtcgggg cgtcgccaat ttctggtttt gacagtggct
    12361 tctctacctt tgctcagggc tttgtcgcgc tggggagttt ccgtctctct tacatcacct
    12421 tctacgcgtt gattgcggtg gcgttcgtct gggtgttgtg gaacaaaacc cgcttcggta
    12481 agaacatttt tgccattggc ggtaacccgg aagcggcaaa agtatctggt gtcaacgtcg
    12541 gcctgaacct gctgatgatc tacgcgttgt ctggcgtgtt ctatgccttt ggcgggatgt
    12601 tagaagccgg acgtatcggc tctgccacca acaacctcgg ctttatgtat gaactggatg
    12661 ctatcgcggc gtgcgtggta ggcggtgtat cgttcagcgg cggtgtgggg acggtgattg
    12721 gcgtggtgac cggggtaatt atttttaccg tcatcaacta tggcctgacg tacatcggcg
    12781 taaacccata ctggcagtac atcatcaaag gggcgattat tatcttcgcc gtagcgctgg
    12841 attcactgaa gtacgcgcgt aagaagtgaa atgattgtgg tccgcattcg gtcataaacg
    12901 cacataacat catgatctgt gtggttttgt aggccggata aggcgttcac gccgcatccg
    12961 gcgacaggag ccaaatgccg gatgcaacgc tggcgcgttt tatccggcct acaatgagct
    13021 caacatactg atttgtatgg ttttgtaggc cggataaggc gttcaagccg catccggcga
    13081 caggaaccaa atgccggatg cgtctctggt tttacaacgt taccgggtgt tctttctcgc
    13141 ctttcttaaa cttcacttcc ccgagctcaa tacaacccac cgggcagacg tgaccacaaa
    13201 gcagacaacc cacacatttc tcggtattac aatgcggtgt gcgggttttc tcgctccatt
    13261 ccatcgcctg gtgaccgccg tcgtaacagg aaatataaca gcgtccacag ccaacacatt
    13321 tatcaagatt gatacgggga tagacaatat aactgcggtc taaatcttcc gccgggacaa
    13381 tattgttatt cgccagacct accatctcct gcagcgaatc aaatccctga tcggcgagat
    13441 aatgcgacaa cccgcttgcc atatcttcca ctatccgata cccgtactgc atgatgccgg
    13501 tggtcacctg taacgttgct gcgccgagca ataaaaactc agccgcatcc tcccaggttt
    13561 caatgccgcc gataccgctg attgggaaat cgcgcagttc tggatgggtg cgcatttgct
    13621 gaatgaagcg cagggcgatc ggttttaccg ctttaccgga atatccggaa atactcgatt
    13681 ttccgttaac gatcggcata ccgattttct gattaagatc gatattggtg atggatttaa
    13741 cggtgttaat cgccgcaatg ccatctgcgc cgccgcgctt cgccgccagc gccacttcgc
    13801 acatatcacc gatattcggc gtcatcttcg ccagcattgg cagcgtggaa ccccgtttca
    13861 ctgcccgaca atatttttct accagctccg ggctttgccc gacatcgcta cccatcgcat
    13921 gagaagtcat ttgcggacag gagaagttac actcgatcat atccgcgcca gcttcttgca
    13981 ccaggcgcgc cagctcctcc cattgctgct cattttcccc catgatcgaa gcgatcaata
    14041 ctttgtccgg gtaatcttcc ttcagctgac gcagggcggc cagattctct tccaacggat
    14101 gttcagcaat ctgctccata tttttgaagc cgataaaacc ggtatcttct ttcaccagat
    14161 gatcaaaacg cggcgagact tcgttggcga taaaaaagcc gatcgtttta aacaccacgc
    14221 cgccccaacc tgtgtcgtag gctttggcac acatctcata gcagttgcct acaggcgaag
    14281 aagagaggca gaacgggttg ggaaacttca cgccgcaaaa agtaatcgaa agatctttcg
    14341 ttaacatgag caagctccct ctaaatagtg atgaatcgcc tcggcggctt ctttcccggt
    14401 tttcacggca tagaccacgg ttttgtcacc ctcaacaata tcgccagcag caaagacttg
    14461 cggatcgcgg gtctgataat tttgtgtttt gatggtgtta cgctgcggct ctaactccgc
    14521 aaaggcatcc agtctggcat gctgaccgac ggcgagaata attttatctg ccgccatcgt
    14581 cagttcgccc ggtaaccgta catgcttaaa cgtgacttta ttgccttcca cggctactgg
    14641 cgtgaatcca tcgatgatcg aaacgcccag ctcccgggcg ctggcaaact ctttttcgct
    14701 tgccggaaac tcatctaact cttcacgcgc tacgcaggtt accgcctgac agccgagaac
    14761 tttcagcgtg ctggctacgt ccatcgcaac atcaccgccg ccgataatta atgcgctttg
    14821 tggaatgctg atatcgcctt gcgcctgtcg tgcacgttgc aggaagtcca cggcaatctc
    14881 aacgtcgcta tgctcaaaca gcgataaccc ggaaccgctc gataacccca cggtgaccag
    14941 taccgcgcgg ttttctgctt tcagctgctc aagggtgagt gtgttaccga cttcgttgtt
    15001 gcacttaatg gtcacgccca ttttttcaat acgggcgatc tctgcatcca gcactgattg
    15061 cggtaaccgg aattgcggaa taccgttacg caaccagcca ccggggtgcg cttctttctc
    15121 ataaatcgtg acgtcataac cctggtttgt cagtgttaca ctggcctgca atccggcagg
    15181 acctgcgcca ataatcgcga ctttgccgag cgttttagta ccgggctgat aaatttccat
    15241 cccagtttgt tgttcaaaat cagtaacaaa acgctgtaag cgaccgatat caatgggagt
    15301 atcaacaccg gcacgggtac aaccgctttg acataatttc tccgtcgggc acactctggc
    15361 acaaacggca ccgagggcat tattttcgcg aattgtctcg gcagcgcctt taaaattacg
    15421 aaagtagatt gagcgaataa atttccccgg atcggtctgt gccgggcaag cctgactaca
    15481 gggagcgtca tgacataata aacagcgaga ggcttcttta atcgccagta aaggcgtaaa
    15541 agccggagtg agttcatcca gataattttg ttgcggcatg tgatcttcct taatgctatg
    15601 agtaattaag gcgaacgcta aataaatatt gcgtgctgat attaaggcta tgcgcgtggg
    15661 gaaatttaaa ctgtgaaagg attcacaaat agctgcggtt cagaatgtta tatccgcagc
    15721 ttattgagtg aagtaataat tattctcaat aattaacaga aaagagaaaa tattaacgat
    15781 agaaaataag ataaacaatc atgccgatag ataaaattcc gaagccgata tatgccacca
    15841 tataagcgaa aaagagtccg ggattttttg ctcgccagac atacaccggt tccggtgcat
    15901 ttttaacccg tttatcaatc gcgcctgcgc gccagccttt ccatgcttcg cggaaacaaa
    15961 atatcgggat aaggaaaaaa acggcaacga caaaaaactg ctgtacatcc ataactactt
    16021 tccttgtttc ttttgcaatt caagtaactg ttcaggcgtg acggcgggat acccctgcgc
    16081 atcttccggc acctggtgca tagccggaat agggaccagc ggccctaaaa accgcggttc
    16141 acgtttaaaa atataaaggt cagccagcgc accgaaacgg gcggcaaatt cgcgaatacg
    16201 tactgacagc atatctttcg gtgacggtac ggcataacac tgagcctgaa tccccatatg
    16261 cagcgcaata aataatgctc gctcacagtg gaaacgttgg gtgataatga tgaaatcatt
    16321 agtatcgaaa actttgcgtg tacgcacgat ggaatccagc gtacgaaagc ctgcgtaatc
    16381 gagaacaata tctgataggt cgacaccagc agcgattaaa tctttgcgca tggtcatcgg
    16441 ctcattataa ctttgcaatg cgttatcgcc gctcagtaat agataattta ccttaccgct
    16501 gttataggca ttaatcgctc cttgaatgcg gtagcgataa tactgattaa ttacgccagt
    16561 acgataatat tttgctgttc cgagcaccac accgacctgg cggtagggga gatcctgcaa
    16621 ttcgtcgtag atataaggcg cggttttcca gctcatccag cgatcgaggc cgagcacagt
    16681 caacagcagc aagccgatca ggactaacag gctgaggaac acgcgcttta acatgcagga
    16741 gactcgatct aaggaaaatt tccatcaggc tactttaccc gctggttttg cgcaagaaac
    16801 ggcaaactgt ttgccgggat tttcgacacc aggagagggc tggcaagccg gacaaggcgt
    16861 tcacgccgca tccggcacca ggcttaagcg agaagtacgc ggtcgatact gtgacagccg
    16921 agagctttca gtgttgcgga ggtggcatcc cactgaatca acggcgcatc ggctttttct
    16981 gccagaaccg cgcgctggat atccgggtaa tcataaccct tgagattcaa tagaattaac
    17041 gctccctgca acggtggcag agtcgggttg aatgcggcgt tttcagcgta gctgccgctg
    17101 aaaatacgac cgtctttaca ttccagcgcg acaccgcttg gcgacttact gtaaggcatg
    17161 tgcgaacggt ttgccgctgc aatcgccgcc tgagaaagcg catcaccagt cagcgcatag
    17221 ccgtgatcct gctcgtccat cagcagcgtt ttaatctcca gatctttcgg cccaaaggca
    17281 tccggcagat agtcacgcag cgcgtgtgcc tcgcggcccg gcagatgaat acgcagatcc
    17341 agaccgctgt tcagttcatt cataaactga cggcagtgac cacaaggcgt gtagttaacg
    17401 gtgatggctg caagcgcttt ttcaccactc aaccaggcgt ggctgatcgc gctttgttcg
    17461 gcatgaacgg tttgctgcat tgtcgcacca ataaattcca tattggcacc gaaataccag
    17521 gttccgctca caccgcgcgc aattgcgcca acattaaaat tcgacaatgg cgtacgcgca
    17581 caggccgccg ccagcggaag tagtgcgaat gccagcgcgt cttcgtccag ccccgttgcg
    17641 ctcttcagcg atgagacttg ctccccggtc aacaaagcgg ggaagtactt gtctgccaga
    17701 ataggttcca gtgcagattg caagttatcc gcaagttggg caaaagcggt ttgaaaacgt
    17761 ggatgcatgg cgttgcctca taatcatgta atgggataac agtttacgaa cccgttatgg
    17821 ctttatatgt gatctgaatc tcattaatta tgcaacgcgt gaaacttaca tcaaaaatgc
    17881 gcgacgcatc gcaaatttta gcccattact gccagaataa tcgggaaaag gaacggtgcg
    17941 atcagcgaag taattatccc gcataacacc agcgctagcg aactaaatgc accttcctga
    18001 taatccagct cggcgcagcg cgccgtaccg agggcgtgcg aggcagtccc cattgccaga
    18061 ccgcgcgcag ctttggtacg aatacgcatc gcattaagca atgtatggcc aaataccgcg
    18121 ccgaggatgc cgacgaaaat cacgcaaact gcgctgattg ccggaatacc gccaatactg
    18181 ccgccaaccg ccattgcaat aggcgtggtg actgatttcg gcaggattga cgcggcgatt
    18241 tccggtgaag cgcccatcaa caatgccacg gaagtccccg tcaccatcgc aaccacgctg
    18301 ccgataaaac agatggtgat gatcgatttc cagcgcgcgc ggatctggtg tagctgctca
    18361 tataaaggat aggccagcgc gacgaccgcc ggttgcagca gatcgtttag cacttcgcta
    18421 cctttaaagt agctgtcgta agagatgccg gtcagcatta aaaaaggaat gatcaccacc
    18481 attgctacca gcaacgggtt aagcagggga aacttatacc gtgccgccag tttgcgggcg
    18541 gcaaaaaaga caatcaacgt cagcggtaat gaccaccaga tattcgccat cattcttctg
    18601 atcctttctg acctacgact ttacgttcac cgtgtaccag ttgcgaactc cagctcacca
    18661 ccagaaaaac caccagcgta ctgattgcac aggaaaccac taccgggcca aactgtgcgc
    18721 ggagcaaatc aaaatattgc atgacgccta cgccaatcgg cacaaacaat agcgccatat
    18781 agcgaatcag tacgtagcac cccggattga cccatttcgc cggaagaatt tgcaaggcca
    18841 gcaggacaaa caggatcagc atcccgatga tgctgcccgg aatggttacc ggtagcaggg
    18901 aagcaatgaa aatgcctgca tacaggcagg cataaatcag gacgaaagcg cgtaaatatt
    18961 gccagataat gttcagtgtc ttactcataa tcaacacccc agatgaaatg gattcatcat
    19021 acaattaatt cgtaaaacgt gctaccgatc acattaagaa atcagcgctt attttttcgc
    19081 tccccggcat tacgcaatgc gcaacgaact caggcataca tcaagcatag agagggatta
    19141 cagtttgcag caatccctct ttgcagacct ttaatccgtt gaccataggc gctaacatgg
    19201 cgcctcattt ttttgcgggt gaagatatgc gtgtgttact ggctccgatg gagggtgtgc
    19261 ttgactctct ggtgcgtgaa ttgctgaccg aagttaacga ctacgatctg tgcatcaccg
    19321 agtttgtccg cgtggtggat caactgctgc cggtaaaagt ctttcatcgc atttgccctg
    19381 agctacaaaa cgccagccgg acaccatctg gtacgctggt gcgcgtgcag ttgttaggtc
    19441 agttcccaca atggctggca gagaacgccg cccgtgcggt ggagttaggt tcctggggcg
    19501 tggatctcaa ttgcggctgc ccgtcgaaaa cggttaacgg tagcggcggc ggcgcgacgt
    19561 tactcaaaga tcctgaactc atctaccagg gtgcaaaagc gatgcgtgaa gctgtaccgg
    19621 cgcatttgcc cgtcagcgtg aaagtgcgtc tgggctggga cagcggtgag aagaaatttg
    19681 aaatcgccga tgcggttcaa caggctggtg ctacggagct ggtggtgcat ggacggacaa
    19741 aagagcaggg ttaccgcgcg gagcatattg actggcaggc gattggcgag attcgccagc
    19801 ggctgaatat tccggtgatt gccaacggcg aaatctggga ctggcagagc gcgcaacaat
    19861 gcattgcgat cagcggctgc gacgcagtga tgattggtcg cggggcgctc aatattccca
    19921 acctgagccg ggtagtaaaa tataacgaac cgcgaatgcc gtggccggag gtggttgctt
    19981 tgctgcaaaa atatacccgt ctggaaaagc agggcgatac cgggttatat cacgttgcgc
    20041 ggattaaaca gtggttgagt tatttgcgta aagaatacga tgaagcaacg ggattatttc
    20101 agcatgttcg ggtgttgaat aattcccctg atattgcaag ggctattcag gcaattgata
    20161 tcgataagct gcgataatag tggaatcgca gtttatcaga gacaatgaat cgcttattca
    20221 gcttcatc
//
LOCUS       DAZKIV010000082        20123 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZKIV010000082 DAZKIV010000000
VERSION     DAZKIV010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.082"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(204..950)
                     /gene="cutC"
                     /locus_tag="ACHOT6_002856"
     CDS             complement(204..950)
                     /gene="cutC"
                     /locus_tag="ACHOT6_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFP9948042.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIIDAAGPLAVTFHRAFDMCANPLNTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLYHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(964..1530)
                     /gene="yecM"
                     /locus_tag="ACHOT6_002857"
     CDS             complement(964..1530)
                     /gene="yecM"
                     /locus_tag="ACHOT6_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFP9948043.1"
                     /translation="MANWQSINELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPYEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1746..3479
                     /gene="argS"
                     /locus_tag="ACHOT6_002858"
     CDS             1746..3479
                     /gene="argS"
                     /locus_tag="ACHOT6_002858"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFP9948044.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEIDEEQLAAAPVIIRKDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(3521..3565)
                     /gene="yecV"
                     /locus_tag="ACHOT6_002859"
     CDS             complement(3521..3565)
                     /gene="yecV"
                     /locus_tag="ACHOT6_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFP9948045.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            3656..4144
                     /gene="yecT"
                     /locus_tag="ACHOT6_002860"
     CDS             3656..4144
                     /gene="yecT"
                     /locus_tag="ACHOT6_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFP9948046.1"
                     /translation="MFKFLVLTLGIISCQVYAEDTLIVNDHDISAIKDCWQKNSGDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4266..4658)
                     /gene="flhe"
                     /locus_tag="ACHOT6_002861"
     CDS             complement(4266..4658)
                     /gene="flhe"
                     /locus_tag="ACHOT6_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFP9948047.1"
                     /translation="MRALLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELDGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4658..6736)
                     /gene="flhA"
                     /locus_tag="ACHOT6_002862"
     CDS             complement(4658..6736)
                     /gene="flhA"
                     /locus_tag="ACHOT6_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFP9948048.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLACWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLGTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6729..7877)
                     /gene="flhB"
                     /locus_tag="ACHOT6_002863"
     CDS             complement(6729..7877)
                     /gene="flhB"
                     /locus_tag="ACHOT6_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFP9948049.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVCV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8079..8723)
                     /gene="cheZ"
                     /locus_tag="ACHOT6_002864"
     CDS             complement(8079..8723)
                     /gene="cheZ"
                     /locus_tag="ACHOT6_002864"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFP9948050.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8734..9123)
                     /gene="cheY"
                     /locus_tag="ACHOT6_002865"
     CDS             complement(8734..9123)
                     /gene="cheY"
                     /locus_tag="ACHOT6_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFP9948051.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9138..10187)
                     /gene="cheB"
                     /locus_tag="ACHOT6_002866"
     CDS             complement(9138..10187)
                     /gene="cheB"
                     /locus_tag="ACHOT6_002866"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFP9948052.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPAMLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10190..11050)
                     /gene="cheR"
                     /locus_tag="ACHOT6_002867"
     CDS             complement(10190..11050)
                     /gene="cheR"
                     /locus_tag="ACHOT6_002867"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFP9948053.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(11069..12670)
                     /gene="tap"
                     /locus_tag="ACHOT6_002868"
     CDS             complement(11069..12670)
                     /gene="tap"
                     /locus_tag="ACHOT6_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFP9948054.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12716..14377)
                     /gene="tar"
                     /locus_tag="ACHOT6_002869"
     CDS             complement(12716..14377)
                     /gene="tar"
                     /locus_tag="ACHOT6_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFP9948055.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYLFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(14522..15025)
                     /gene="cheW"
                     /locus_tag="ACHOT6_002870"
     CDS             complement(14522..15025)
                     /gene="cheW"
                     /locus_tag="ACHOT6_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFP9948056.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(15046..17010)
                     /gene="cheA"
                     /locus_tag="ACHOT6_002871"
     CDS             complement(15046..17010)
                     /gene="cheA"
                     /locus_tag="ACHOT6_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFP9948057.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(17015..17941)
                     /gene="motB"
                     /locus_tag="ACHOT6_002872"
     CDS             complement(17015..17941)
                     /gene="motB"
                     /locus_tag="ACHOT6_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFP9948058.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(17938..18825)
                     /gene="motA"
                     /locus_tag="ACHOT6_002873"
     CDS             complement(17938..18825)
                     /gene="motA"
                     /locus_tag="ACHOT6_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFP9948059.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLATVLRQKSAETSKMMQCVKVTLLSNLNGYVPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(18952..19530)
                     /gene="flhC"
                     /locus_tag="ACHOT6_002874"
     CDS             complement(18952..19530)
                     /gene="flhC"
                     /locus_tag="ACHOT6_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFP9948060.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(19533..19883)
                     /gene="flhD"
                     /locus_tag="ACHOT6_002875"
     CDS             complement(19533..19883)
                     /gene="flhD"
                     /locus_tag="ACHOT6_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFP9948061.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         4798 a         5561 c         4920 g         4844 t
ORIGIN      
        1 gttttagtaa tttaaatccc ataattaatg tgaatatata aaatattttt agcacaacgc
       61 tatgttcact gtcacccgct caacagatca cggtaatcgt tcgtttttta tactgctcag
      121 ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc atcatattgg
      181 gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat tcctttcatt
      241 tcagcaaccg ccgccccgtc tacgacataa cgcgaatact cgtccgcgtg tgcatctgat
      301 gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc tcccgcggag
      361 ctatggactt ccagcactcc ggcatcgagg aagtggtaca ggttttctgc acggactcct
      421 gctccggcca taatgattgg agcatcacga tgggcaataa gttccataat ttttgataaa
      481 ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc caattccgcc
      541 agattattga gtgtatttaa agggttagcg cacatatcga aggcgcgatg aaaagtcact
      601 gccagcggac cggcagcatc tattattttt tccattcgtg gcatatcgac attaccgtca
      661 acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggactgt gcgcacatcc
      721 tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg tgggcggata
      781 atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacacc caacgacggc
      841 gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc gttttgctgc
      901 gccgttagcg cgcattccat gctgtaacag caaatttcca gtaatgccat ttttgctcct
      961 taattacgcc gactgctcgc tggcaacaat ctcttcaatg gaccacggat gaaatttaat
     1021 ggtcgttttg ccatcggtga ccgccagcgt cggattcggt aatcgttcat gctcaccttt
     1081 tggcgaactg gttttaactg agatccccgg caggctgagt ccttcatcag aaagcaatgc
     1141 cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa tatgttccca
     1201 accttcgtac gggtaacgtt tttccccggg ccacggtagc tccacaatgg aaaactgcca
     1261 gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc cattaatcat
     1321 attttctgac aaaagctcgc cgcactgttc aaacccgcga cgccagcgtt cagcggtgac
     1381 gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat ccaaccccag
     1441 acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg caatatcctg
     1501 tagctcgttg atagattgcc agttcgccat aatcactctt cgtcgttcaa taaaagcgtt
     1561 aatttaccct gttgccctgc gccaaccaac cgctgatttc acgccgcttc tgatgcaata
     1621 gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt gcagtatact
     1681 cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca atgtaaggta
     1741 ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg attgcggcag
     1801 gcgcgcctgc ggattgcgaa ccgcaggttc gtcagtcagc aaaagttcag ttcggcgact
     1861 atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg cgacaattag
     1921 cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt gagatcgccg
     1981 gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat gttcagcagg
     2041 cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt gtggttgact
     2101 actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct accattattg
     2161 gtgacgcagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc gcaaaccacg
     2221 tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag cagcagcagg
     2281 aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat gcgaaaaagc
     2341 attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta aaactgcaaa
     2401 gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc atgacgcaga
     2461 accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg atgggcgaaa
     2521 gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa ggtctggcag
     2581 tagaaagcga aggggcgacc gtcgtattcc ttgatgagtt taaaaacaag gaaggcgaac
     2641 cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc actgatatcg
     2701 cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat tacatcgact
     2761 cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca ggctatgtac
     2821 cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggcaaa gacggcaaac
     2881 cgttcaaaac ccgcgcgggc ggtacagtga aactggcaga cctgctggat gaagccctgg
     2941 agcgtgcacg ccgtctggtg gcagaaaaga acccggatat gccagccgac gagctggaaa
     3001 aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc aaaaaccgca
     3061 ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat accgcgccat
     3121 acatgcagta tgcatacacg cgtgtattgt ccgtgttccg taaagcagaa attgacgaag
     3181 agcaactggc tgcagctccg gttatcatcc gtaaagatcg tgaagcgcaa ctggcagctc
     3241 gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg ccgcatgtaa
     3301 tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag cactgcccga
     3361 tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca caactgacgg
     3421 cgaagacgct gaagctgggt ctggatacgc tgggtattga gacagtagag cgtatgtaat
     3481 caatttttcg tgagagtgaa gcctgatcag ggttacctga tcaggctttt ttattgccat
     3541 ctaaatgtat tctgaaaatg gacatgccac cgttttctca ctgttgggta agaagtcaga
     3601 agcgtaatat ccagcccctg ggaaacggta acggttcaat ttaaggaata ccgcagtgtt
     3661 taaatttctt gtattaacat taggcattat ctcttgccag gtttacgcag aagatacgct
     3721 tatagtaaat gatcatgaca tttcagccat caaagattgt tggcaaaaaa attcaggtga
     3781 tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc tcgtcgatgc
     3841 gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc cacgcacagg
     3901 tgtaaaaaaa cctgataccg aacaacttaa cttgcttaaa aaatcacagc gagcctggct
     3961 ggactttagg gacaaagaat gtgaattaat cctttcaaat gaggacgttc aggatttaag
     4021 tgacccttat tctgaatcag aatggctctc atgtatgatc atacagacca atacgcgaac
     4081 tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt tgacaagagg
     4141 ataattcaca tatttttggc atgttttgtt gcaagctatt cctgataaat gattgcaaca
     4201 aaacatccag cctttttcac tgagtcatta aacatactcg cgagcgcgta atttttattt
     4261 gtccttcagc gataattcac aatcacttca ttccgttgta cctttagcgg cggaattaac
     4321 cgcccaccgc caggcacttc ccagataaag cgcaacggtt ctgctgccgc tataccggaa
     4381 aaggccacgg tggttccgct ctgcccatct aattcgacac aacgagattg cgaacacaag
     4441 cgaacccgca gtcctgcagg tgtcgggccg ataagctgat accgccacgc caccagcgtc
     4501 atcaatcctg aagcaggttg tcgagtagaa agaggcgctg acgacatcga ctcaccgcga
     4561 tgatttagcg taattccgac actacttgcc tgccacatcc cctccccggc ggcttgcact
     4621 agcagcggaa acaataatat tgctaataag gctctcatta tttgccgcca atcgtcgccg
     4681 tcatgcggat atgtcggtta tcagacagtt ccagattcga caggaccact aactgcggca
     4741 agctgcggcg caggaagcga gacaataatg gtcgcagcgc gtggttcacc aacaatactg
     4801 gcggcgcacc cagcatctcc tgacgggata gcgcttcctg agtttgcgcc agtaaacgat
     4861 ccgccagccc tggctccagt cctcccccgc cctgcagcgc ctgtagtaac aaacgttcca
     4921 gcggtgtacc gaggccaata acatggactt catctttgcc aggaaaccac tgctgggtaa
     4981 tcgcccgtcc caacgccacg cgcacgacgg cggttaattc atgtggatcg ctttggatgg
     5041 gcgcatgttc cgccagcgtt tcgagaatgg tgcgcatatc gcgaatcggt actttttcat
     5101 cgaggagatt ttgcagcact ttatgcagtg tggtgagcgt gacgacgcca ggaacgagat
     5161 cttccgtcag ctttggcatc tcctgggcga cgcgatccaa cagctgttgc gcctcctgac
     5221 gaccaaacag ctctgcggca tgctggctaa tgaggtggtt aagatgcgtt gctaccaccg
     5281 tgctggcctc aaccactgtg tacccctgaa tctgcgcctg ttcttttagc gcactttcaa
     5341 tccagatagc attcaggcca aatgccggat cgacggtcgc ctcaccaggt aacgtcccgg
     5401 cagcggttcc agggttaatc gccagccagc gccccggata agcatcacca ctgccaatct
     5461 ccacgccttt catcaaaatg cgatagcggg caggttgcag atccatattg tcgcgaatgt
     5521 gcaccactgg cggcagaaat cccatctcct gggcaaattt cttgcggata ctgcgtatac
     5581 ggcccaacaa ctcaccatcc tgctggaaat cgaccatcgg gatcagtcga taacccactt
     5641 ccattcccag agaatcttcc agttgtacat cgttccacgt cgcttcgaca acggtattat
     5701 tctctgccat ttttaccggt ttgggttcgg caggcgcttt ttgttcgcgt ccgcgtatcc
     5761 aacaggccag cccgagcaat ccggcagtga acagcaaaaa taccaggttc ggcattccag
     5821 gcaccaggcc gagtaaaccg agcacggcgg cgcttaacaa cataacgctt gggttactga
     5881 aaagctgatt caccatctgc tcgccaacat cctgatcggt gctgacacgc gtaacgatga
     5941 cccccgcggc ggtagaaatc accagcgccg gaatttgtgc caccagaccg tcgccaatgg
     6001 tcaatagcgt ataactttcc gccgcgtgtc ccatgctcat gccatgttgc agcacgccga
     6061 ccagcaaccc gccgacaacg ttaatgacca tgatgaggat cccggcgatg gcatcgccgc
     6121 gaacaaactt acttgccccg tccattgagc cgtaaaaatc ggcttcctga gtcacttcgg
     6181 agcggcgttt tttcgcctca tcttcaccaa tcaatccggc gttaaggtcg gcgtcaatcg
     6241 ccatctgctt acccggcata ccatcgagaa caaagcgcgc acccacttct gcgatacgcc
     6301 cggcaccttt ggtaatgacc ataaagttga tgatcacgag aatgacaaac accacgatac
     6361 cgatagcgaa attgccacca acgaggaagt gaccgaacgc ttcgaccacc ttccctgccg
     6421 ccgccgcgcc ggtatgccct tccattaaaa tgatacgggt tgaagccacg ttaagtgcca
     6481 gacgcaacag cgtggtaaac aacagaatgg tcggaaacgc agcaaactca agcgtgcgct
     6541 gggtaaacat cgccaccagc aacaccatga tcgacaaggc aatattgaag gtaaacaaca
     6601 ggtcgagtat gaatgcgggc agtggcagca ccatcatcga caagatcaac aggatcaaaa
     6661 tcggtccggc aaggatctgc cattgtgtcg atttcaggtt tgcgggcagg cgcagcatcg
     6721 cggccagatt actcatgggt cggtttctcg ttaataaaat ccagggcttc cggcaccgga
     6781 agatgagtcg gttgtacagg gcgctgtcca ccagccagac gccagcgttt cagttgccag
     6841 acccaggcca gcacttccgc caccgcggcg tacagttgac ccgggatttg ttgaccaatc
     6901 tccgcatgtc gatacagcgc tcgcgccagc ggcggcgctt caagcgtcgg gacgttattt
     6961 tcagcgccaa tttcacgaat gcgcagcgcg accagccctg cacctttagc gaccactttc
     7021 ggtgcgctca ttttgttttc gtcatactgc aacgctaccg aatagtgggt cgggttattg
     7081 acaatgacat ccgctttcgg cacatcggcc atcatccgac gccgtgcggc agctcgctgc
     7141 atctgacgga tccgtccttt aacatggggg tcgccttcgc tttgtttgaa ctcatcacga
     7201 atatcctgcc gcgacattcg cagctttttc aggtgactga agatttggaa aaagacgtca
     7261 aatcccacca ttggaatgac accaagcacc accagcagtg cgcatagccc taccaaatcc
     7321 atcgcattac ccatggcggt aatcggagac tcggccatta agcgcatcat ctgcggccag
     7381 tgatgccaga gaaaaaaccc cgtcacgctg ccaaccagga tggttttcaa aattgcttta
     7441 agcaactccg cgccagtctg agccgagaac atccgtttaa tgcccggtag cgggttgagt
     7501 ttggaaaact tcggctgcaa ggatttgccg ctaaatacca gcccccccag catgaccgga
     7561 gaaatgagcg ccaccagcac cacgccgcta atcagcggca gcagcgccag catggcttct
     7621 ctgatcagca gaataatctg cccgaggatc agattcgggt cattgataat actatgatca
     7681 aaatgcagcc cagcggagag catgcccgac aatcgacggg ccagcgacac accgccaaac
     7741 cagataacac acacgcccac taacaaaatc agcaacgagg tcagttcacg ggaacgcggg
     7801 atttgcccct cttcccgcgc tttttctagt cggtgaggtg tgggggcttc tgttttgtcg
     7861 tcgctctcgt cagacacgtc gccaatcctg gaaagagtat taagcttaaa tgatgccaga
     7921 gcgcaaagcg ttcaatggtt tgagtaaggg gcaaaacagg cgggatttag ggcttttgct
     7981 gccacacatc aagcatagtg tgcgtttgtc ggatgcggcg tcatcgcctt atcagaccgc
     8041 ctgatatgac gtggtcacgc cacatcaggc aatacaaatc aaaatccaag actatccaac
     8101 aaatcgtcca cctgatcctg actggctacc acgccggctt tgctggtatc gacctgaggt
     8161 ccattaagca aactctggtt ttcacgtttt ggacgcgact cctgttccgg gatgttttcc
     8221 aacagcacca tcagcaactg gcgttcgatc tcctgaatga catccatcat ccgcttaatg
     8281 acctgcccgg tgagatcctg aaaatcctgc gccatcatga tttccagcag ttgcgcgtta
     8341 gtaaagctgg tatgcgcggg tacatctgcc agaaattgtc gtgtatctgt taccagttca
     8401 cgggcgtcgg caaggtcaat cggatcggca aaccagtcat cccaacgttg ggttaacgct
     8461 tttgctgatt tctccatttg atcctgatgc ggttgtgacg cctcaacact gttcagcgcc
     8521 cgctccgcag cctgggcggt catctgcaca acatagtaca aacgatcgcg cgcatcgggg
     8581 atggcttccg ccgcttcggc aatggcctga tccagcccca gttcccgcaa actgtcgcgc
     8641 agcatacgcg tcaggctgcc gatgcgcgca atgatatcac cagctgaatg ctcgtcagca
     8701 ggtttgattg atggttgcat catagtcgca tcctcacatg cccagtttct caaagatttt
     8761 gttgagtttt tcctccagcg tcgcggcggt aaatggcttc accacatagc cactggcccc
     8821 cgcttgcgcc gcagcaatga tgttctcttt cttcgcttct gcagtcacca ttaacactgg
     8881 caatgctgac atcgcgccat ccgcacgaat tgttttcagc aattccaggc catccatgtt
     8941 gggcatgttc cagtcggaga taacaaatcc ataaccgcct gcctgcaact tattgagagc
     9001 gtcgacgcca tcttccgctt cctcaacatt attgaatccc agctctttca gcaggttacg
     9061 cactatgcgt cgcatggtag aaaagtcatc cacaaccaaa aatttaagtt ctttatccgc
     9121 catttcacac tcctgattta aatacgtatc gcctgtccgg cactaatttt tgccagcatt
     9181 tgctggctta cctggctaag atcgaccact tcgcaaacac cacccatatt gatggcctcg
     9241 cgcggcatgc cgaacaccac gcaacttgct tcgttttgcg caagggtcca tgcccccgcc
     9301 tgacgcatcg ccaacattcc cgccgcgccg tcgttgccca taccggtcag gatcacccca
     9361 accgcattac gccccgcctg tttggcgaca gaatggaaca gcacatctac cgaaggccga
     9421 tgacggttaa ccgccgggcc atcgtgaatt ttgatttggt aatttgcgcc actacgcgcc
     9481 agctccatat gccgatcgcc cggcgcaata taggcatgcc ccggcaagac acgttctccg
     9541 tcttcggctt ctttaacccc gatctggcaa agcttattaa gtctgtcggc aaaagagcgg
     9601 gtgaaaccag gcggcatatg ctgggtaatt aacattgccg ggctggaaag cggcaacggt
     9661 tgcagtacgt gacgaattgc ctcagttcca cccgttgaag caccaatcgc aatcagtttt
     9721 tcagaactca acaacggacc cgccttcagc gttgtcggtg ccgacaatgg cttatgtgct
     9781 gcaaggctcg cctttgctgc tgtacgcacc ttttcagcaa tcatttcgct atacgccagc
     9841 attccttcgc gaatacccag ttgcggtttg gtgacaaaat ctatcgcccc cagctccagc
     9901 gcgcgcagcg tgacttctga ccctttgccg gtcagggaag aaaccatcac aacgggcatt
     9961 ggacgcaaac gcattaattt ttcgaggaaa tccagtccgt ccatccgcgg catttcaacg
    10021 tccagcgtca atacatcggg attgaatttc ttaatcaaat cacgcgcgac cagcggatca
    10081 ggcgcggtcg ccaccatttc catgtcgcta tggctgttga tgatttctgt catgatctgg
    10141 cgcatcagtg ccgaatcatc gacagataac accctgattt tgctcatcgt taatccttac
    10201 ttagcgcata caccgtctga ccacgcagcg tgaagcggcg ctcaaggtgg ctaaagtttt
    10261 cagagtgacc cgcaaacagc aatccgtcgg gtttaaggag cggaacaaag cggcgcaaaa
    10321 tctcctgctg ggtattttga tcgaagtaga tcatgacgtt acgacagaag atcgcatcaa
    10381 acggccccgg cacggtgtac tgtttcgcca gtagattcag cggggcaaaa tcaacatagt
    10441 tcgccagctc ctgacgcacg cgtaccagcc cttcatgcgg tcccgtccct cgcatgaaat
    10501 accgttgcag ttgctgcggc gtcaggtttt tcaactcttc atggcgataa ataccgcttc
    10561 tggctttttc cagcacttcg gtgtcgatat cactggcaaa cactttccag cgtccgggcg
    10621 cggtgcccaa tgtgtcagcc agcgtcatcg caatgctgta cggctcttcg ccggtcgaag
    10681 ccgccgcgct ccatacgcga tactcgccag aacggcgacg tgcgtgatcc gcgagcagag
    10741 ggaaatggtg tgcctcacgg aaaaatgccg tcagattcgt ggtcagcgaa ttgataaacg
    10801 cctgccactc accgctgtgc tgattagatt ccagcaagtt cagataatga ccgaaatccg
    10861 tcagtcccag cgaacgcaaa cgacgaacca gtcggttgta aaccatgtcg cgtttatggt
    10921 cagccagaac gatcccggct cgttgataga tcaattgact tatccgccga aaatgcgcgt
    10981 cggaaagcgc caggcgctcg gtcatctgta acaataaaga cgtttgccca cagggcagag
    11041 atgaagtcat agcgccttct caatcacttc aggataccac tggcgcaatt tgtaactgcg
    11101 ccgactcatg tcgtgctact tcatgttctt caagggtaaa taccgccaca cgcgacgaaa
    11161 gatggtcggc ctgattcgcc agttgttccg ttgccaccgc cgcttcttct accagcgagg
    11221 cgttctgctg agtcacctga tccatctggc tgacagcctg tgcaacctgc tcaatccccc
    11281 gccgttgttc ttccgacgcc gaagcaattt ctcccataat gtcgttcacg cgggtcaccg
    11341 aactgacaat atcggtcatg gtcgcggcgg cgttattcac cagtttcgaa ccctgctgga
    11401 cacgattgac tgactcttcg atcagccctt tgatctcttt tgctgcctgc gcgctacggc
    11461 tggcaagatt gcgcacttca cctgccacta ccgcaaaacc acgcccctgc tctccggcgc
    11521 gagccgcttc cactgccgca ttcagggcca gaatattggt ctggaaagca attccgtcga
    11581 taacgctgat aatgtcgcca attttttgcg agctggtggc gatctcctgc atggtgtgag
    11641 tcatggtact gacctgaaca ccgcccgcct gcgccgttgt cgcggcattt tttgccagtt
    11701 ccgacgcctg tcgtgcgtta tcggcgtttt gccctaccgt ggcggttaat tgctccatac
    11761 tggcggccgt ttgtgccagc gatgccgcct gctgttcggt acggctggag agatcgttat
    11821 tgcctgcgac aatctccgcg ataccaatgt gcatctcatg gcttccctta cgcacatcac
    11881 tcaccgtccc acgcaaagcc tgctgcatgg tcttcaggct ggcaaaaatg gcggtgatct
    11941 cattacgacc atataccgca atcggacgcg ccagattacc cgcagcaatg ctgtcaaaat
    12001 ggctaccgat aatggccagt ggttgaacaa tcatcttgcg cgtccaccac agcgcactgc
    12061 tgatgtagat tgccgcaaca ataatcatgc tgataaacac cagcgccgaa atctgatagt
    12121 tacgctgact ttgcgcactg gcggcttcca gcacatggtt gatctccagc tgccaggcct
    12181 caaagttaac gtcaaacgca ttctgtgatc cctgcaccgg cgcagtgagg aaatccgaaa
    12241 gttgattact ttcaagccag gtcgcctgat gttcgagatc gttgtgccag cgggcaaaac
    12301 ttttctccgt ctctttttgc aatgccctga cgtgctcgtt gcccgcagtc atcgccataa
    12361 aacttttaaa cagcgtggtg gattgcgtca gactggcgcg cgccgtcgtc atcaacgttt
    12421 taatgtcatc cgccggatag ctaagtgccg tcagagtgcc cgctttgttc agcgcggtac
    12481 tggcctgtaa cattaccgcc cgagtttgcg ctaatgccgc acgttgctga ttgctctgct
    12541 cgacctgatt cagtcgttgc aaatcgtcgc gaaatgccca aaaagacatg ccgttactgc
    12601 caatctgcaa gatcccgcag agaatcaaaa ttaaaaacag cgtggtcgaa attcgaatac
    12661 gattaaacat caacgctccc catcaggcgg caatgaccgc gttagtaaat actcgtcaaa
    12721 atgtttccca gtttggatct tgttcagcaa ttcgcagtcg tggctgtgcc ggtggttgct
    12781 cactggcagg acgggatggt gtttgcggtt tattggtgag tgggctggct gccagacgga
    12841 acgcggaaac cgcttgcgtt aaacgactcg cctgttcttc cagcgcagcg gcggcggcag
    12901 ctgattcctg caccagcgat gcgttctgtt gcgtgacgcg atccatttcc gaaaccgcca
    12961 atgcgacttg atcgatgcca cggctctgtt catccgatgc cgatgcaatc tcgcccataa
    13021 tgtcagtcac gcgagtgaca gcattgacga tattgttcat tgtttccccg gcgctttcga
    13081 ccagcaccga accggtatca acgcgtgaga cggagtcttc aatgagggct ttgatctctt
    13141 ttgccgcctg ggcgctgcga ctggcaagat tacgcacttc acccgccacc acggcaaaac
    13201 cacggccctg ttcacccgca cgcgcggctt caaccgcggc attcagcgcg aggatattag
    13261 tctggaaggc aataccgtcg ataacgctga taatgtcggc aattttcttc gaactatcgg
    13321 cgatctcatg catcgttttc actacgccat ccaccacttt gccgccgtgc tgggcggtgt
    13381 cggaggcact ttgcgccagt tgcgaggcct ggcgggcgtt atcggcgttt tgcttcactg
    13441 tcgcggtgag ctgctccatg ctggcggcag tttcttccag cgcggatgcc tgctgttcag
    13501 tacgggagga aagatcggtg ttgcccgccg caatttcacg ggtaccggca tagatggcat
    13561 ctgaaccttc gcggacatga gtgacagtgt cagtcaaaga gcgttgcata tgtgaaacgc
    13621 tctgcgccag gtcgcccatt tcactgcgcc cgtcaatggt cagggtattc gccaggttac
    13681 caccggcgat ttcgcgaatg tgagcaataa tttttgccag cggagtaagc aacatacggc
    13741 gaatgccgta ccacgccacc agcagaatca ataccaccac cagcgcgata accgccagtt
    13801 gccactgggc aaataggtaa tcatctgcgt tgtcagtgac gatatcgcga tacagttttt
    13861 cactgctgag ggcgtactga gcaaacgctt cgcccattgc attttgcatt ccctgggttg
    13921 gctgagcgaa ataagctcca gtattgccat aatctagata atcaatcagt tcagttaacg
    13981 ctgtgtaata gtttttatat ttttcatcaa tattacgact ggtagcgacc atttcaggta
    14041 acggtgccat gcttttgaat tttttataat gcgtcgctgc ctgcgccaat gttttcctgg
    14101 cgctatcgag caattcaact ttggcgttac tttgttgatt ggaggaatcc atcatcatcc
    14161 gtaccgctga acgactcagg ttaatgcgcg tttgcagcat taaatcccag gttgacgtca
    14221 gctcgccctg ctgttcccgt aattgattgg aaaccacaaa gctcttctgg ctatggtgaa
    14281 gggaagaaaa aaacagactg ccggaaataa gctgtaacag tgcgaatacc cccagcacca
    14341 ttaccaacag cgtgactacg cggatacggt taatcataag gcaccttcct gaaaacaagt
    14401 tgatctcgtt atcggcaaga aggggggaaa ctttattgct gatgccaccc gccgcgaaat
    14461 tgaaataaaa aacccgatgc gcagatcatc gggttcattt caattgagga aatcgggaga
    14521 attacgccac ttctgacgcc gcgctatcta acagcgccat ctcttcgctg ttcagcagtt
    14581 tttcgatgtt caccagaatc aacatacggt cgcccagtgc gcccagtcca gtgagatatt
    14641 ctgttgaaag cgtcacggca aattctggtg ccggacgaat ttgctccgcc gtcaatgaaa
    14701 gcacgtctga aacgccgtca accacgatgc caaccacccg ttgtccgaga ttcaggacga
    14761 taactaccgt gttgtcgtta tagtccacat ccacctggct gaacttaatt cgtaagtcaa
    14821 caatcggcac aataaccccg cgcagattcg tgacgccttt gataaacgct ggcgtgttcg
    14881 caatccgtgt tacctgatcg tagccacgga tctcctgcac tttcaggata tcaataccgt
    14941 actcttcatc accaagggta aataccagaa attcctggcc tgacggctcg ctggccagct
    15001 ttgttacatt cgtcataccg gtcatattgt taccttttta ctcattcagg cggcggtgtt
    15061 cgccatacgt tgttcgcggt ttatcgcctg caaggcggag acatcaacaa tcagtgccac
    15121 gctgccgtcg ccaagaatgg tcgcagcaga aatgccgggg actttgcgat agttactttc
    15181 aaggttttta accacaacct ggtgctgacc aattaattga tccaccagca aggcgtagcg
    15241 gcgaccgcca ctttgtaaga tcaccacaat tccctgggtg gcttcggttt tcgcgcccgc
    15301 gacgttgaac actttccaca gttcgacgat gggcagatat tcgccccgca cttccagcac
    15361 ccgctcgccg ccggccagtg gatgcagatc ggcttcacgg ggttgcagtg attccatgac
    15421 agcattcagc ggcagaatga aaacttcatc cgcaacgcgt acggacatgc cgtcgaggat
    15481 ggccagcgtc agcggcagta aaatgcggat cgtagtgcca gtaccctgct tcgactggat
    15541 ttcgacatgg ccgcccatct cctggatatt acgtttaacg acgtccatgc cgacgccgcg
    15601 cccggagacg tcggtgacct gctctgccgt ggagaagcct ggtgcaaata tcagcatcgc
    15661 gacttcgtcg tcgctcatgt tttcgctgac agtcaaacct tgcgaggccg cttttgccag
    15721 aattcgctca cggtttagcc ccgccccatc gtcggtcact tcaatgcaaa tgttgccgcc
    15781 ctgatgttcg gcagacagaa ttaaatttcc gacgctgttt ttacctgcgg cgagccgttt
    15841 ttctggcagt tcaataccgt gatcgaggct attgcgtacc aggtgggtca gcgggtcgat
    15901 aatgcgttct atcaggctct tgtcgagctc ggtggaactg cccaccagcg tcagttctac
    15961 ctgcttgccg agttttcccg ccagatcacg caccagccgg ggatagcgac taaagacata
    16021 ttccatcggc atcatgcgaa tcgacatcac tgattcctgc aaatcacggg cgttacgttg
    16081 taactgcccc atgctggtaa tcaaatcacc atgattaacc gggtccagtt cgctggaacg
    16141 ctgggcaagc atggactggg tgataaccag ctcgccgacg aggttaatta attgatcaac
    16201 cttttctacc gctacacgga tgctggtgga ttcactgctg cgcgtcgttt tttcccgctc
    16261 cacgcggccg gttggcgctt gttcggctgc cagtttaagc acaggtgggg tagctatttt
    16321 tggcgagact tctactgttt caaaggtaat ctgatcggct tcaatcacaa aacagagtac
    16381 cgctgtgatg tcatcttcgg cgatgtcgcc cggtaatatt gccgagagcg aatccgcccc
    16441 tttcaccacg tcagttaagg ttgtcagatg tcccagttct tcttccagca ggtcgacttc
    16501 cccggccttc aggcgcgaaa ggataattcg tcgcggcgac tgactgcgac tctgctcatc
    16561 ttgcggttca cttttggcaa ccacacttaa tcgggtcact gcggatggcg tttcgccttt
    16621 cgcttctaat gccagttgac gcaaggcctg gcagatataa tcgaagctgg cggcatccgg
    16681 ctcttgcgac tgtttataag cgtcgagctg ttcttgcatg atgtccttcg tttccaaaaa
    16741 cagattgata atgtcggtgt tgagttgcat ctcacctcgt ctggcttcat cgagcaggtt
    16801 ttccatcaga tgcgtggttt cctgcaaaac gctgaagcca aaagttcctg cccctccttt
    16861 gatcgagtgg gcagcccgaa agatggcatt caattgttcg gcatctggcg cttccggctg
    16921 caaaaccagc agatgctgct ccatatcagc caacagttcg tccgcttcat caaaaaatgt
    16981 ctgataaaaa tcgcttatat ccatgctcac gctgtcacct cggttcggct gatggcattg
    17041 tgggaacact gacctgtggt gcaacctcag gtttttccag ggcgcttact ggctcattct
    17101 ggctttcggc gttttcatgc aaaatggcct gttcggcttg tttgttcagt accagcaggc
    17161 tgatgcgacg gttgacggca tcatcaggtc cgcgatcgct taagcgcatc gttgccgcca
    17221 tgccgacgac acgtaacact ttgccgctat ccaaccctcc gaccatcagt tcgcggcggg
    17281 atgcattggc ccgatcggca gaaagctccc agttgctgta acctttctca ccgctggcgt
    17341 agggaaaatc atcggtatga cctgaaaggc taatacggtt gggaataccg ttcagtacag
    17401 gcgcgatggc gcgcagaatg tcgcgcatat agggttcgac atcggcactg ccggttctaa
    17461 acatcgggcg attctggcta tcgatgatct gaatacgtag accttcctgg accagatcga
    17521 ttttgagatg gggacgtaac gcccgcagtt tcggatcgga ctctatcaac tggtcgagat
    17581 caccccgcaa tttccgcaat cgactttgct ccatgcgttt tttcagctct tcgatgttcg
    17641 gctgcttatt cacttccccc tggctttggg tgtaatcatc accaccgccg ggaattgggc
    17701 tttcactatt agaaatgcga tcgccgcccg taaccgcagt cgccagtgga gtccggaagt
    17761 actccgcaat ctgaatcagc tcttttgggc tggagatgga gatcagccac atcaccagaa
    17821 aaaaggccat catcgcagtc ataaagtcgg cataagcaat cttccacgat ccatgtgctg
    17881 ccccgtggct tttggctttg cgtcgtttga cgacaataat cggatgcgct tgattcttca
    17941 tgcttcctcg gttgtcgtct gttgttgcgg atttttcacc gcacgcacat gctcttccag
    18001 ttcaataaac gacggacgtt cgctggaata gagcgttttg cgaccaaact caacggcgat
    18061 aggcggtacg taaccgttca gattagaaag cagagtgact ttgacgcact gcatcatttt
    18121 gctggtttcg gcgcttttct gacgtaaaac agtcgctaat ggggaaataa atccgtaagc
    18181 caataaaatg ccgaggaaag tccccaccat cgcatgtgcg ataagcgccc ccagctcggc
    18241 ggcaggacga tcggctgaac ctaacgcgtg aacgaccccc attacagccg caacaatacc
    18301 aaacgccgga agtgagtccc cgaccagcgc cagactgttc gccgggactt ctgcctcgct
    18361 ttcgtgcgtc tcaatctctt catccatcag ggcttcgatt tcgaaggtgt tcatgtgacc
    18421 gctgataatc aggcgcagat aatcgacgat aaaatcaagc atgacgctat ccgcgaggat
    18481 gcgtgggtag ctggcgaaga tctcgctctc acggggattt tcaatatcac gttccagcga
    18541 aaacatcccc atctgccgcg atttcgccat caaccgataa agcagagcca gcagatccat
    18601 atacattgct ttggtgtatt tggagcgacg aaacagcaac ggcagcgctt tcagcgtgcc
    18661 tttaatcgct ttgccattat tgccgacgat aaacgaccca atccctgcac cggcaataat
    18721 caccagttca gcgggttgat agagtgctcc aaggcttcca ccggtcatca aataaccgcc
    18781 gaaaactgta ccgagaacaa ccaggtaacc taataagata agcacgacat catccttcca
    18841 ctgttgacca tgacaggatg ttcagtcgtc aggcgttaac gcgcgattgg ggcaaaaaaa
    18901 agcagcggta cgtcgttacc gctgctggaa tgttgcgcca caccgtatca gttaaacagc
    18961 ctgtactctc tgttcatcca gcagttgtgg gataatatcg gcaggattct gggaaagttt
    19021 acgtcttttt actgcccggg atggcggttg acataagctg caggcaaagc tgccaacagg
    19081 ctggtgagcg tgggtaataa aattgccgcc gcagcagttg cagctggaaa gttgcagtaa
    19141 tccactttca acaaaccgca ccaatgtcca ggcacgggtt aatgccagca gtggtccttc
    19201 ttctgcttgt gggcactgtt caaggtataa acggtaggct ttgatcaccg catcgacgcc
    19261 attacacaaa cctgttttca gtaaaaactg ccatgcatta cagaacatcg aagcatgaac
    19321 gttttgttcc caggtcataa accagtcggt tgagaatggc agcatgcctt tcggcggtgg
    19381 gcttccgcgc agttctttat aaagttttat caggcgtccg cgacttaact gtgtttcgct
    19441 ttccagcatc tgcaaacgag cgcccagggt gatcaattcc attgccagct gaatatcccg
    19501 cgcttcctga acaatgcttt tttcactcat gatcaggccc ttttcttgcg cagcgcttct
    19561 tcaggctgat taacatcatt cagcaagcgt gttgagagca tgatgccggt atgaatttgc
    19621 tggagatcgt caacgcggga atcttgcgtc aactgagtaa tcgtctggtg gctgtcaaaa
    19681 cggaagtgac aaaccagttg attggtttct gccagcttaa ccatttgcgg aagagtcagt
    19741 gccgctaacg ttgtcgccat ttcttcattt atgccgagac gaaacatagc ggacgctttg
    19801 tcctgaacaa tcaaacgctg tgcaagtagt aaatatgaca agttgatgtc ataaatgtgt
    19861 ttcagcaact cggaggtatg cattattccc acccagaata accaacttta tttttatgcg
    19921 gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt agcattagaa
    19981 tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt ccttacaaat
    20041 agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact gtactcgtca
    20101 ctacacgcac atacaacgga ggg
//
LOCUS       DAZKIV010000083        19783 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZKIV010000083 DAZKIV010000000
VERSION     DAZKIV010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.083"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(333..530)
                     /locus_tag="ACHOT6_002876"
                     /pseudo
     CDS             complement(333..530)
                     /locus_tag="ACHOT6_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309848.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspO"
     gene            527..706
                     /locus_tag="ACHOT6_002877"
                     /pseudo
     CDS             527..706
                     /locus_tag="ACHOT6_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612101.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1116..1217)
                     /locus_tag="ACHOT6_002878"
                     /pseudo
     CDS             complement(1116..1217)
                     /locus_tag="ACHOT6_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310605.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1441..1566
                     /locus_tag="ACHOT6_002879"
                     /pseudo
     CDS             1441..1566
                     /locus_tag="ACHOT6_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203849.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1626..2132)
                     /gene="yciE"
                     /locus_tag="ACHOT6_002880"
     CDS             complement(1626..2132)
                     /gene="yciE"
                     /locus_tag="ACHOT6_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9948062.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(2178..2678)
                     /gene="yciF"
                     /locus_tag="ACHOT6_002881"
     CDS             complement(2178..2678)
                     /gene="yciF"
                     /locus_tag="ACHOT6_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFP9948063.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2764..2943)
                     /gene="yciG"
                     /locus_tag="ACHOT6_002882"
     CDS             complement(2764..2943)
                     /gene="yciG"
                     /locus_tag="ACHOT6_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9948064.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(3324..4130)
                     /gene="trpA"
                     /locus_tag="ACHOT6_002883"
     CDS             complement(3324..4130)
                     /gene="trpA"
                     /locus_tag="ACHOT6_002883"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFP9948065.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(4130..5323)
                     /gene="trpB"
                     /locus_tag="ACHOT6_002884"
     CDS             complement(4130..5323)
                     /gene="trpB"
                     /locus_tag="ACHOT6_002884"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFP9948066.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(5335..6696)
                     /gene="trpCF"
                     /locus_tag="ACHOT6_002885"
     CDS             complement(5335..6696)
                     /gene="trpCF"
                     /locus_tag="ACHOT6_002885"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFP9948067.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(6697..8292)
                     /gene="trpD"
                     /locus_tag="ACHOT6_002886"
     CDS             complement(6697..8292)
                     /gene="trpD"
                     /locus_tag="ACHOT6_002886"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFP9948068.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(8292..9854)
                     /gene="trpE"
                     /locus_tag="ACHOT6_002887"
     CDS             complement(8292..9854)
                     /gene="trpE"
                     /locus_tag="ACHOT6_002887"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFP9948069.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9876..9969)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9946..9990)
                     /gene="trpL"
                     /locus_tag="ACHOT6_002888"
     CDS             complement(9946..9990)
                     /gene="trpL"
                     /locus_tag="ACHOT6_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFP9948070.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            10128..11009
                     /gene="rnm"
                     /locus_tag="ACHOT6_002889"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             10128..11009
                     /gene="rnm"
                     /locus_tag="ACHOT6_002889"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFP9948071.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11006..11626
                     /gene="yciO"
                     /locus_tag="ACHOT6_002890"
     CDS             11006..11626
                     /gene="yciO"
                     /locus_tag="ACHOT6_002890"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFP9948072.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            11727..12599
                     /gene="rluB"
                     /locus_tag="ACHOT6_002891"
     CDS             11727..12599
                     /gene="rluB"
                     /locus_tag="ACHOT6_002891"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFP9948073.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12639..13229)
                     /gene="cobO"
                     /locus_tag="ACHOT6_002892"
     CDS             complement(12639..13229)
                     /gene="cobO"
                     /locus_tag="ACHOT6_002892"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFP9948074.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(13226..13984)
                     /gene="yciK"
                     /locus_tag="ACHOT6_002893"
     CDS             complement(13226..13984)
                     /gene="yciK"
                     /locus_tag="ACHOT6_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFP9948075.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            14204..15253
                     /gene="sohB"
                     /locus_tag="ACHOT6_002894"
     CDS             14204..15253
                     /gene="sohB"
                     /locus_tag="ACHOT6_002894"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFP9948076.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(15289..15540)
                     /gene="yciN"
                     /locus_tag="ACHOT6_002895"
     CDS             complement(15289..15540)
                     /gene="yciN"
                     /locus_tag="ACHOT6_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFP9948077.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15920..18517
                     /gene="topA"
                     /locus_tag="ACHOT6_002896"
     CDS             15920..18517
                     /gene="topA"
                     /locus_tag="ACHOT6_002896"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFP9948078.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            18728..19702
                     /gene="cysB"
                     /locus_tag="ACHOT6_002897"
     CDS             18728..19702
                     /gene="cysB"
                     /locus_tag="ACHOT6_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFP9948079.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4922 a         4802 c         5071 g         4988 t
ORIGIN      
        1 tcataatcta ttgatgttaa tggtttttat tgtttgttgt tcggcttttt atactaattt
       61 gagcgaaaca aaaagataaa aagttgtttt tcgtgtcttt aagtgatacc agatggtatt
      121 gcgctaaatg taccaatggg tataccaaga gcactaaaaa caaaggggca accactttgt
      181 aaccccttga ttatttgcag aagcgcagaa aatcgaactc tggaaccctt tcggggcatc
      241 ctttttcaag aatgaagaaa aaaataacta aaaaatcaag ttgttaggaa gttattcatg
      301 gctagtgtac caactctttt ctgggctaat agctatgatt tgtttgtatt atttgtctct
      361 gtctctatta tttttgacat ccatgcgact tctgatattg ataatctcag ttgtggagtt
      421 ctgtctgcgc ttccgggagg acgcatatca aaactacagg atcttctgtt aaacactgta
      481 aagccatcta cattgcatgt tgttgagtct aagatacaat ttttagtgtt acgataatgc
      541 tagggcggaa agtttcttcc actcactaaa agtcgaatgc atttacggct aatattttat
      601 gagtcgggaa ataatgcaga cggctgtgtt taattagatc tagtgtgatt acaatcggtg
      661 gtgccgacac agtacttgtg gctgtctcag cacggaactg ttttgaaaaa cagaaccttg
      721 attagggctg tgtccacatt acgtaggtag gatcacccca caggcatcac atgtcatgca
      781 tggattagga ttgaatgata aatcgtaaag aaaactgcaa cacctcgcgt gtagcacctg
      841 gttagcgaac tttcgaaagt acgagtattg tatgatttcc agcagttatc aattttacgt
      901 attaatcagt gaatcaacaa ctcgtcagca gactctcttt gttgtggaat tttttggaac
      961 gtgtataggg tagaaatttt tgttttggga catggttggg acgccgtagt acatacgaac
     1021 tctgccgaat aacgtatgat attatttttt aatctcttgg aatacattat agaaacagaa
     1081 tgctcttggg cgatttttag tgattgttta aatagccctg tcatgcagtt aaagtggcgg
     1141 gcatcctctt caaggatggt gatcctcaac cgcacccatt taggatgcga ctattgtggt
     1201 agaacaatgt aaaccatagt taatctgcgt gcggatacat gcttcataga taggatattc
     1261 tggtttgggt tgtgaatgtg ttggtcatcg agtggacaat aaggtaagag gagtaactcc
     1321 tgcaaaacaa aaaaagagtt caacaaaatc aatcagttaa aaacacgaga gatctgtata
     1381 aaaaagcatc gtaaattctg tatttttcct ttaaatataa tatgttattg agttttgtaa
     1441 aatctaccag tgtggtacat ggatatcgat accaccgcca attataagct aggcggtgca
     1501 cagcaacacg atagcgtacg cctcgatccg tgggtgttta tgttctcagc aggatatcgt
     1561 ttttaattcc gcacaaaaac gaccccgtaa tatacggggt caataaggac atggtataaa
     1621 gggtattatt tcttcgcttc tacgccatca gtttcagaac gaattaaaaa tttctcagtt
     1681 gtttgcggaa tatgctgaat cagccagtcg gccatttgct tttcctcatt taaaatcgct
     1741 tcgatgatcg gaattgagac tgtatcaccg gcattttttg ctgctgctaa tagtgaggtg
     1801 taacaggcga tttcaaattg ctcgaagaca tatccgctaa tagagccttt aactatttca
     1861 tcagaaggga atataccacc gattgactgc ccaagcgcag ccattttact catggaatct
     1921 ttaatgactg aacgtgaaat gtcattacga tcaagaatag tttccagttg aacaatctgg
     1981 tttttggttt cactaagatg ttgttcaata cgagcgcgta gttcaggata attatctata
     2041 cggctggcca tggattcaag catagattcg gcttgctttt ccattgcgtg ggcgtcacgt
     2101 aaccagtcat gataatgttc aatacgattc atgaaaatgt cctctttata aaatgactaa
     2161 aagttaaaat tcatatttca ggctttattt tcggctttct tatttacgtt attaagggcc
     2221 agatcagtca gtttgatgtc ggtggccttt tcttcttcca gggtttcttt cagaagcttc
     2281 gctgctttac ggtaacctaa ttgttcagcc agcgtcgcta atgtcccgta actggcaatc
     2341 tcataatgct cgactttctg tgctgcggca atcagtgcgg catcacgcac ttcgtttttc
     2401 tcggtacttt cgatgacctc attagcttct tcaataagac cttccattgc cacacatttc
     2461 atgcgcttaa ttttcaggtt cgattcggac tccacaactt gatcaatacg ttcaatctgt
     2521 ccatgagttt cctcgaggtg cgcatgaaaa gcctgactta atttttcatt tgatgttgct
     2581 cttgcgagtt ttgccagtgc ccgggttaat tgtttttctg cgctgtaggt atctgaaagc
     2641 aggtgaataa atacatcttc aatggtcttc atattcatat ttttctccag tgaaatcact
     2701 gcgagccata cggcccgcag atgcaattat ctttatcagc tgatattaat aattaacggt
     2761 gaatcaggat ttgcctgatt tattaccacc gctttgttga ccgccttttt tacccgcttc
     2821 agatgcgcgt tgcggatcat ttttaaaatt accgccgcta tgctgaccgc ctttacggcc
     2881 tgcgtcggat gccttctcac ggtcttcggc gaaatttcct gaaccaccac gatgttcggc
     2941 catgttattt ctcccgttgc gttgcattgt ttcattaata tgagtgttgt gtgtcgacac
     3001 tcattaaaat tagtcgctaa tgaggattag tcaaattaag cgcaacgaga agatagaggg
     3061 aaaatatatt ttgaggaaca ttctggatat attaacaatt acctgaggaa taagtgactt
     3121 agagaaaatt attgatttta ctggtgttat gttgcgggat taatttgttt gattatatta
     3181 ctgttgggcg gaaaaatgac gtaagttgac gttcgaccgg ggtaaacgaa acggtaaaaa
     3241 gataaatatt aaatgaattt aggatttttc cggcttcatt aaagaaagtt aaaatgccgc
     3301 cagcggaact ggcggctgtg ggattaactg cgcgtcgccg ctttcatcgg ttgtacaaaa
     3361 actttcagtg ccgccagcat tttctctggc tcattaatat gttgctcgat gattttaaca
     3421 atggccgaac cagaaatcgc gcccgcagct cctgcatcaa tcgctgcttt tacctgatcc
     3481 ggggcggaaa taccaaatcc ctgcaatgga ggtgcagcgt tgtactcttt cagcttcgca
     3541 accagatgat tgaggggtaa cgcggcgcgg ttttctgcgc cggtcacgcc tgctcgtgac
     3601 agcaaatagg tgtaaccacg accgtaagag gctatctggc gcagcaggtc gtcatcggca
     3661 tttggcgggc agatgaagat aggtgcgaca ttatgacgca acgcggcctg gcggaagggc
     3721 gcggactctt caactggcac atcggcaacc agcaccgaat cgacgccgac tttttcgcac
     3781 tgggcataaa actcatcaat gcctttgtta aacaccagat tggcatacat caacaggcca
     3841 atgggaatgg tcgggtgttt ctggcgaatc agtgccagca tttcaaaaca ttgtgccgga
     3901 gtcacacctg ccgcaaaggc gcgcagagtg gcgttttgaa tcgtcgggcc atccgccagt
     3961 gggtcggaga aggggatacc taactccagc gcgtcagcac cggcttcaat tagcgtatcg
     4021 ataattttca atgactgctc aatgcccgga tcaccgagcg tgacgaaagg aacgaatgcg
     4081 ccttctttgc gctccttcaa ctgggcaaac agagattcgt agcgttccat cagatttccc
     4141 ctcgtgcttt caaaatatcg tgaacggtga agatgtcttt atcgccgcga ccggaaaggt
     4201 taaccaccag tagctgctct ttatccgggt tttcgcgcat cattttcaac gcatgggcca
     4261 gggcgtggga ggattccagc gccgggatga tcccttcgtg caggcacagc gttttgaagg
     4321 cttcaagggc ttcatcatcg gtaatagaca cgtaatcagc gcgtccagtg ctgttaagat
     4381 acgcgtgttg tgggccgaca gacgggaaat ccagtccggc ggagatggag taagattctt
     4441 caatctgccc gtcttcggtt tgcatcatcg gcgctttcat accgaaatag atacccacgc
     4501 gaccatgttt tagcggtgcg ccgtgctcgc cagtttcgat accgtgacca cctggctcca
     4561 caccaatcag gccgacgttg gtttcattga tgaaatcagc aaacatgccg atggcattcg
     4621 aaccgccgcc aacacaggcg ataacggcat ccggcaggcg accttctctt tccagaatct
     4681 gcgctttggt ttcttcgcca atcatccgct gaaactcacg cacaatggtc ggataaggat
     4741 gcgggccagc tgcggtgccc agcatatagt gcgcggtttc gtaactaccg gaccagtcgc
     4801 gcagcgcctc gttacaggca tctttcagcg tcgcggaacc gctatgcacc gggatcactt
     4861 ccgcacccat taagcgcata cgaaaaacgt taggcgactg gcgttcaacg tctttggcac
     4921 ccatataaat acggcatttc aggccgagca gggcgctggc aagggccgac gccacgccat
     4981 gctgaccggc accggtttcg gcgatgattt cggttttacc catccgcttc gccagcaacg
     5041 cctgccccag cacctggtta gttttatgcg cgccgccgtg cagcaaatct tcacgcttga
     5101 gatacagcgt ggtgttcgtc ccggctgtaa tgttctggca tttggtcagc gcggttggac
     5161 gcccggcata gtttttcagc aggtcgttga actgagcctg aaattcagga tctttttgcg
     5221 cactgacaaa agcttcttcc agctggcgca gagcaggcat caggatttgt ggcacgtaca
     5281 tgccgccaaa ctcaccaaaa taggggttaa gtaatgttgt cattgttcct ttccttaata
     5341 tgcgcgcagc gtctggaaaa ccgaggccaa aagacgtgcg tctttgatgc ccggttgcga
     5401 ctctacagca gaattaaaat caagtccggc gcagccggtt tgtgccgctt ccacgcagtt
     5461 atctgcgcct aagccccccg ccagcagaac gttgccaagc gattgaccat ttaatagtga
     5521 ccagtcaaaa cgttgcccgc ttccaccctg gccgttgtct aaaacatatt tatcaacgtg
     5581 ctgaaactcg cgggcgggca gggtttcacc gacgcttaat gctttccaga tggcaacatg
     5641 tgctggcaga gcttcacgca gcgtatcgat atacagctgt tcttcattac catgcagttg
     5701 cactgccgcc agcgataaca ccttagcttt gtccaccaca tcggcaatat cgtgattgcg
     5761 gaacacgcca acatactgca acggtgccgc agccatcact tcctgcgcct gttcaacgtt
     5821 gacgcaacgc ggtgatgtcg caacaaaaat caacccaccg taaatcgcgc ccgcgtcata
     5881 agctgcttta gcatcttgcc cacgcgtcag gccacatact ttattctcac ccagcaacac
     5941 ccggcgcacg gcggcgtgca aatcgtcatg ggccatcaac gccgaaccaa tcagaaaacc
     6001 gttagcgaag tggcttaact cgcgcacctg agcgtaagta ttgatgccgg attcgctgat
     6061 taccgtcacg ttgtgcccca gtttcggcgc aagctcgcgg gtacggttga gatcaatcga
     6121 caaatcacgc agatcgcggt tgttgatgcc aacgaccttt gcccccaatg caatggcacg
     6181 ctccagttcc tcttcattac tgacttcggt cagcacaccc atctccagac tgtgggcgac
     6241 ggcggcaagc tggcgatatt gttcgtcatc cagtactgaa agcattaata agcaggcatc
     6301 agcctggtaa tagcgcgcca gatagatctg gtaaggatcg ataatgaagt ctttacataa
     6361 aatcggctgc ggggcgattt ggctgacgat ggggaggaaa tcaaagctcc cctgaaaata
     6421 tttctcatca gtcagcaccg aaattgccga agcgtaatgt ttataaatgg cggcaatgcg
     6481 tgctggatcg aaatcatcac ggatcacgcc ttttgacggc gacgctttct tgcactccag
     6541 aataaacgcc gtgcgcgcac cctgtagcgc atcataaaaa tgtcgcgtgc tcggctgaac
     6601 ctcattctga aaactggcca gcggttgctg ctgtttgcgg gtttctaccc aaatcgcctt
     6661 gtctgcgacg attttcgcta aaacggtttg catcatttac cctcgtgccg ccagtgcggt
     6721 aactctgtcg taagcggaac cactgcgcag tacctcaaga acggtttgcg cattggcttg
     6781 cagatcttca tggccatgca ggcgcattaa catggcgacg ttcgcagcga cggctgcttc
     6841 atgggcggcg tcgcctttac cttgtaacaa acgtgttaaa atgtcacggt tttcttccgg
     6901 tgttccgcct gccagttgct cctggtggta gggtgtcagg ccaaaatctt cagcggtcaa
     6961 ttgatagctc ttaatttcgc cgtcatgcag ctcggcaacg attgtcggcg cgtgtaatga
     7021 aacttcatcc atcccgccgc tgtgcaccac cgccgcgcgt tgatacccca gcacgcgcaa
     7081 ggtttcggca atcggcagca ccagttccgg actataaaca ccaattaacg ccagcggcgg
     7141 atgcgccggg ttaatcaatg gccccagcac attgaacagg gtgcgggttt ttagttgctg
     7201 gcgaaccggc attgcatggc gaaatccggt gtgatatttc ggtgcaaaga ggaaacatac
     7261 gcctaactca tccagcgcct ggcgcgattt atcggcgttc atatcaagat taataccgaa
     7321 cgccgccagc agatcggacg aaccagattt actggagacg ctacggttgc cgtgtttcgc
     7381 cactttcagc ccacaggccg cggcgacaaa cgcactggcg gtagaaatat tgatactgtt
     7441 gctgccgtca ccgccagtac cgacgatatc agcaaacaga taatccgggc gcgggaacgg
     7501 cgctgcgttt tccagtagcg cggttgctgc cccggcgatc tcgttcgggt gctcaccgcg
     7561 aattttcatg ctcaccagcg ccgccgccag ttgttccggc ttcagctcgc cacgcaccac
     7621 cgctgaaaac agctggtggc tttcttgttg gctaagcgtc tgcgcctgat acagtttttc
     7681 cagaatcggt tgcagcgtgt tggctggctc tagtttctgc tgcgcccagg ccagcgtttg
     7741 ttccagcagg cgagcgccct gggtggtgag aatggattcc ggatggaact ggaatccaca
     7801 aacgcgatcc gcatcgtgac gtactgccat caccatgcca ttaaaatggg cgttgatggt
     7861 taaaccggcc ggaatgttac tgccaaccag cgagtgataa cgcgccaccg gcagcgggtt
     7921 tgttaatccg gcaaacatcg cctgaccgtc atgttcaatg ctcgacgctt taccgtgaag
     7981 aatttcgccc gcctgaccga catagccccc gtaagcttcg acaattgcct gatgtccgag
     8041 gcaaatgcca ataatgggca gcttgccacg caagcgggtg aggagttccg gcatacaacc
     8101 ggcttcgctc ggcacaccgg ggccaggaga aagcatcagt accggattgc tcatggtcgc
     8161 caggcgttca attaaggtct gcgccggaat atggttgcgg taaatcacca cgttatgccc
     8221 attgctgcgc aactgatctg ccaggttgta cgtaaaagag tcgatattat cgagcagcag
     8281 tatgtcagcc atcagaaagt ctcctgtgca tgatgcgcgg tggcaatagc gcgcagtaca
     8341 gcgcgggctt tattacgggt ttcgtcggct tccgactgcg gaatagaatc aaggactacg
     8401 ccagcaccgg cttgcacggt ggcgataccg ttttccacca gcgccgagcg gatcacaatg
     8461 caggtgtcga gatcgccgtg cgcggtgaaa taacctaccg cgccgccgta gctgccgcgg
     8521 cgacgacctt ccgcctcggc aattaactgc atggcgcgca ctttcggcgc accgcttaac
     8581 gtccccatat tcatacaggc gcggtaagcg tgtagggcgt cgagatcgtg gcgcagttca
     8641 ccgaccacgc gggagaccag gtgcatcacg taagagtaac ggtcaacttt ggtgaggtcg
     8701 gcgacgtagc ggctgccggg ggtgcaaatg cgtgccagat cattacgggc aagatccacc
     8761 agcatcagat gttcagaaag ctctttatga tcggtacgca tttccagttc gatgcggctg
     8821 tcgaggtctc tgtccagcga gccatcggca cgacgaccgc gtggacgtgt tccggcaatc
     8881 gggtagatct caatctggcg gctggtggcg tcatacttga gcgaactttc cggcgacgcg
     8941 ccaaacaggg tgaaatcatt atcctgcata aaaaacatgt acgggctggg attactcttt
     9001 ttcagcacgt aataggccgc cagcggtgac gggcagggca gagagaaacg gcgagatggc
     9061 accacctgga aaatttctcc agcgcgaatc gctttttgca acaaacgcac tacgccaccg
     9121 aactcttcat cgctctgatt acattcacaa cgcatatgcg gcacggaaac caccggcagc
     9181 ggcggcgcgg cttcggtcag ttgctgacgt agttcgttca ggcgagcagt gagacgttgt
     9241 ttttcttctt cattcggagc aaacaggctg gcctgaatac gggtgctttt tttctgatgg
     9301 tcaatcacca tcagagtttc agcgagataa aaacagaaat cagggcagtt attttccgct
     9361 gacagttgcg gtaaatcttc aaatcccgcc acaaggtcat aagagaacag gccgccgaag
     9421 aacattgctt ctcgttcttc cttcggtaca ttcaacagat tctgcaataa acggaaagcg
     9481 tcaaaaaccg aaagggagca taagcgagcg tcttcatcca gcagtggact gacagggggg
     9541 aagcgcagca cacggcagtt tggtaattgt tcattttcca cacccgcagg cagggcgtta
     9601 tccagtagtg tcagcagggc ttcgccgttg ccggaaagtg cctggattgt gacagtgtca
     9661 cctaaagctg taatgcgcag cgcactgtct accagcagca ggctttttaa atcatctttg
     9721 ctgtcgatat ctgcggattc cagcagcagc gttgccggac gatccccaca caactggtga
     9781 aaaagcgcgg tgggattgtc gcgataagcg ccttcgcagg ttagcagttc gagagtcggt
     9841 ttttgtgttt gcattgttat tctctaattt tgttcaaaaa aaagcccgct cattaggcgg
     9901 gctgggtatc tgattgcttt acgcatggtg aatacactgc ccgtttcagg aagtgcgcca
     9961 ccaacctttc agtacgaaaa ttgctttcat tgtcgatacc ctttttacgt gaacttgcgt
    10021 actagttaac tagttcgatg attaattgtc aacagctcat ttcagaatat ttgccagaac
    10081 cgttatgatg tcggcgcaaa aaacattatc cagaacggga gtgcgccttg agcgacacga
    10141 attatgcagt gatttacgac ctgcacagcc ataccacagc ttccgatggc tgcctgacgc
    10201 cagaagcatt ggtgcaccgt gcagtcgaga tgcgcgtcgg caccctggcg atcaccgacc
    10261 atgacaccac agctgcaatc gcgccagcaa gagaggaaat ttcacgttcg ggactggcgt
    10321 tgaatcttat tcccggcgtg gaaatttcca cggtctggga aaatcatgaa attcatattg
    10381 tcgggctgaa tattgatatt actcatccgc taatgtgtga gttcctcgcg cagcagacag
    10441 aacggcgcaa tcagcgggcg cagctgattg ccgaaagact tgaaaaagcg caaatacctg
    10501 gggcgctgga aggcgcacaa cgactggcgc agggcggcgc agtgacgcgc ggtcattttg
    10561 cgcgtttcct tgttgagtgc ggcaaagcca gttcaatggc ggatgtcttt aaaaagtatc
    10621 tggcgcgcgg gaaaaccgga tacgttccgc cacagtggtg tacaatagaa caagctattg
    10681 atgtcattca tcattctggc ggtaaggcgg tgctagctca tcctgggcgg tacaatctta
    10741 gtgctaaatg gctgaaaaga ttggttgcgc attttgccga acaccacggt gacgcgatgg
    10801 aagtcgcgca gtgccagcaa tcgcccaatg aacgtaccca gctggcggcc cttgcgcgtc
    10861 agcatcattt atgggcatca caaggatctg attttcatca gccatgcccg tggatcgaac
    10921 tgggtcgtaa actctggctg cccgcaggcg ttgaaggcgt ctggcagcta tgggaacagc
    10981 cgcagaacac cacagagagg gaattatgag ccagtttttt tatattcatc ctgataaccc
    11041 acagcaacgt ctgatcaacc aggcggtgga gatcgtgcgt aaaggcgggg tgattgttta
    11101 tccaactgat tccggctatg cgctcggctg taaaattgaa gataaaaacg cgatggagcg
    11161 tatttgtcgt attcgccagc tgccggacgg tcacaacttt accctgatgt gtcgcgatct
    11221 ttctgaactc tcgacctatt catttgttga taacgttgcg tttcgtttaa tgaaaaacaa
    11281 cacgccgggc aactatacct tcatcttgaa ggggacgaag gaagtgccac gccgtctgtt
    11341 gcaggaaaaa cgcaaaacca tcggtatgcg tgtgccttct aacccgattg cgcaggcgtt
    11401 gcttgaagca ctgggcgaac cgatgctttc cacttcgcta atgctgccag gcagcgaatt
    11461 taccgaatcc gatcccgaag aaattaaaga ccgtctggaa aaacaggtgg atttgattat
    11521 ccacggcggc tatctcggac agaaaccgac aacggttatt gatcttaccg atgatacgcc
    11581 ggtcgtggtg cgtgaaggcg taggtgatgt gaagcctttc ttataagtgc gtaactctgt
    11641 atactacgcg gccttaaaat ggcgctatac ggcgccattg attctcctgt tcgacgcctg
    11701 tgaaggcgac accaaaagga agctctatga gcgaaaaatt acagaaagtg ctggcccgcg
    11761 ctggccacgg ctcccgccgt gaaatcgaat ctattattga agccggtcgt gtgagtgttg
    11821 atggcaaaat tgctaaactc ggcgatcgcg ttgaagttac ccctgggctg aaaattcgta
    11881 tcgatggtca tctgatttcg gtacgtgagt ccgctgaaca aatttgtcgc gttctggcct
    11941 attacaaacc ggaaggtgag ttgtgtaccc gtaacgatcc ggaagggcgt ccgacggtgt
    12001 ttgaccgtct gccaaagctg cgtggcgcac gctggattgc cgtaggtcgt ctggacgtta
    12061 acacctgtgg tctgctgctg ttcaccaccg atggtgaact ggcaaaccgt ttaatgcatc
    12121 caagccgtga agttgaacgt gaatacgccg tgcgtgtatt tggtcaggtt gacgacgcga
    12181 aactgcgtga tttgagtcgt ggcgtgcaac tggaagatgg tccggcagct tttaaaacca
    12241 tcaagttcag cggcggcgaa gggatcaacc agtggtacaa cgtgactctg accgaaggcc
    12301 gtaaccgtga agttcgtcgt ctgtgggaag cggttggtgt gcaggtgagc cgcctgatcc
    12361 gtgttcgtta cggtgatatc ccactgccaa aaggtctgcc acgcggtggc tggacggagc
    12421 tggacctcgc ccagactaac tatctgcgcg aactggtgga gcttccgcca gaaaccagct
    12481 ctaaagtcgc tgtagaaaaa gaccgtcgtc gcatgaaggc taatcagatt cgtcgtgcgg
    12541 tgaaacgtca cagtcaggtg agcggcggtc gtcgttctgg cgggcgtaat aacggttaat
    12601 caaagtatgc cggacgtcat atccggcatt tttacagatt aataatcaat ccctatttgc
    12661 gccttaacac cggcatcaaa cgcatgtttg atggggcgca gctcacttac cgtatccgcc
    12721 agttcaagaa tatcccgatg acagcctcga cccgtgataa tcaccgtctg ttgatgtgga
    12781 cgttcattta acgcctgcac cacttcttcc agtggcaaat agtcatacgc caccatatac
    12841 gtcagttcat caagcaaaac catatccagt gaggaatcag caagcatccg ctttgcatgt
    12901 tgccagactt cgcggcaggc ggcggtatca gactcgcggt tttgtgtatc ccaggtaaag
    12961 cccgttgcca tcacctgaaa ctcaacgcca tgtggctcca gcagattgcg ttcgccatta
    13021 ggccaggtgc ctttaataaa ctgcacgacg cccacttttt ttccgtgacc aactgcgcgt
    13081 gttgccgtac caaatgccgc ggtggttttg ccttttccat tgccggtaaa gacgatgata
    13141 ataccgcgtt catcctgggc ctgggccaca cgagcatcta ctttttcttt cactcgctgc
    13201 tgacgctgtt ggtagcgttc atcactcatt gggaaattcc tggtttacgg cccggttggg
    13261 cgtcaaaggt catgccggtt ttacggcggc tgtcatcgcc catcagccag aggtagagcg
    13321 gcatgatatc agcgggtgtt ttaagtttct gtggatcttc ggtcgggaag gcgctggcac
    13381 gcattgcggt gcgcgtaccg cctgggttaa tgcagttgac gcgcaggcgc tgctgatatt
    13441 catcggccag tacctgcatc atcccttcgg tggcaaattt cgacgctgca tatgcgcccc
    13501 agttggctcg tccctgacgt ccaacgcttg atgaagtaaa gaccagtgaa ccggcgtccg
    13561 atttgagtaa taaaggaagc agtgcctggg tgagcataaa ggtggcatta acgttgacct
    13621 gtatgacgtc ctgccagacc tgtggatttt gttcgctcat tgggcaaaca tcgccgagca
    13681 atccggcatt atgcaaaaca ccatccagac gcggataatt aacggtaatg cgctgtgcca
    13741 gttgttggca atcttcggac gtgcaggtca gcaaatcgag aataaaccac tgtggctgac
    13801 gtccagtttc ttcgtttatg tggctggcta cctgacgtaa tttttcttca ttacggccca
    13861 acagaatcac tgtcgcacca tagcgtgcat acgtcatcgc ggcttcacgg ccaatcccat
    13921 cgctggctcc cgtcaccagg ataatgcgat cattgagtaa atcttgtttt ggctggtaat
    13981 gcatggctac tcctcaacga cgttgtctgt cgcgctgcca gtgtacttta tgactttggg
    14041 gctttatgcc tgaaacagaa gggcatttca atcagcctgg ggcgataaca ccgcaaaatg
    14101 gatactttgt catactttcg ctgcaataac atctctgcga gacggcttaa catgcctgtt
    14161 gtaaactgtg agccaaagcg ttgtttaacc aaggtgggga ctcgtggaat tgttgtctga
    14221 atatggtttg tttttggcga aaatcgttac cgttgtgcta gcgattgcgg cgattgccgc
    14281 cattattgtc aatgttgctc aacgtaataa acgccagcgt ggcgagttac gggtcaacaa
    14341 tctcagcgaa cagtataagg agatgaaaga agaactggcc gcggcgctga tggactcaca
    14401 tcagcaaaaa cagtggcaca aagcgcagaa gaaaaagcac aagcaagaag cgaaagcagc
    14461 aaaagcgaaa gccaaactgg gcgaggtggc aactgacagt aaacctcgcg tctgggtgct
    14521 ggattttaaa ggcagcatgg acgcccatga agtgaactcg ctacgtgaag agataacggc
    14581 ggtactcgca gcattcaaac cgcaggatca ggttgtgctc cgtctggaaa gccctggtgg
    14641 catggtgcat ggttacgggt tggcggcttc gcagctgcag cgtctgcgtg ataaaaacat
    14701 tcctttaact gttacggtag acaaagtcgc tgccagcggc ggttacatga tggcctgtgt
    14761 ggcggacaaa attgtttccg caccgtttgc tattgtgggt tccattgggg tggtggcgca
    14821 aatgcccaac tttaaccgct tcctgaaaag caaagatatt gatatcgaac tgcacactgc
    14881 cgggcagtat aagcgtactc tgacgttgct gggtgaaaat accgaagaag ggcgggagaa
    14941 attccgcgaa gagttgaacg aaacgcatca gttgtttaaa gattttgtga agcgtatgcg
    15001 tccgtctctg gatattgaac aggtggcaac gggtgaacac tggtacggac aacaggcggt
    15061 agagaaaggc ctggttgatg aaatcaacac cagtgatgaa gttattctta gcctgatgga
    15121 aggccgtgaa gtggttaatg tacgctatat gcagcgtaaa cgactcattg accgattcac
    15181 cggcagcgcg gcagagagcg ccgatcgatt gttgctacgc tggtggcagc ggggtcaaaa
    15241 gccattgatg taaaagacaa acgcgaggct aagacctcgc gttttgcttt aatcaaccag
    15301 atgatatttt tctgaaagca catgggccag gtgtttgaac atattaaaca ccgcggtgct
    15361 tttggctgtt ggcaatcctt gttcatctaa aaagtagtcg ccggtaaata ccagcacgcc
    15421 attacgctgc gtgacgccgg tggcttcaat ccctgcgagc gtatcctcat gctcacgaat
    15481 gattttgttg gcctctttca acagcgtttc gcggtcgatg ggttgtgtct ctttgttcat
    15541 tatttactcc ttaaacaagg acattagtct acgccaggca tggcttgcag acaaatatac
    15601 cacgctggtg gcaagagcgc cttactggta actttggatt ttgcatgtta ataaagttgc
    15661 gtatcggatt ttatcaggta cagtgtgacg ctttcgtcaa tctggcaata gatttgcttg
    15721 acattcgacc aaaattccgt cgtgctatag cgcctgtagg ccaagacctg ttaactcagt
    15781 cacctgaatt ttcgtgaaca gagtcacgac aaggggttga tatccgcaga gaacgagtcc
    15841 atatcggtaa ctcgttgcca gtggaaggtt tatcaacgtg cgacgcattc ctggaagaat
    15901 caaattaggt aaggtgaata tgggtaaagc tcttgtcatc gttgagtccc cggcaaaagc
    15961 caaaacgatc aacaagtatc tgggtagtga ctacgtggtg aaatccagcg tcggtcacat
    16021 ccgcgatttg ccgaccagtg gctcagctgc caaaaagagt gccgactcta cctccaccaa
    16081 gacggctaaa aagcctaaaa aggatgaacg tggcgctctc gtcaaccgta tgggggttga
    16141 cccgtggcac aattgggagg cgcactatga agtgttgcct ggtaaagaga aggtcgtctc
    16201 tgaactgaaa caactggctg aaaaagccga ccacatctat ctcgcaaccg accttgaccg
    16261 cgaaggggaa gccattgcat ggcacctgcg ggaagtgatt ggtggtgatg atgcgcgcta
    16321 tagccgagtg gtgtttaacg aaattactaa aaacgcgatc cgccaggcat ttaacaaacc
    16381 gggtgagctg aatattgatc gtgttaatgc ccagcaggcg cgtcgcttta tggaccgcgt
    16441 ggttgggtat atggtttcgc cgctgctatg gaaaaagatc gctcgtggtc tgtctgccgg
    16501 tcgtgtgcag tcggtggcgg ttcgcctggt ggtcgagcgt gagcgtgaaa ttaaagcgtt
    16561 cgtgccggaa gagttctggg aagtcgatgc cagcacgacc acgccatctg gtgaagcgtt
    16621 ggcgttgcag gtgactcatc agaacgacaa accgttccgt ccggtcaaca aagaacaaac
    16681 tcaggctgcg gtaagtctgc tggaaaaagc gcgctacagc gtgctggaac gtgaagacaa
    16741 accgacaacc agtaaacctg gcgctccttt tattacctct acgctgcaac aagctgccag
    16801 cacccgtctt ggatttggcg tgaaaaaaac catgatgatg gcgcagcgtt tgtatgaagc
    16861 aggctatatc acttacatgc gtaccgactc cactaacctg agtcaggacg cggtaaatat
    16921 ggttcgcggt tatatcagcg ataattttgg taagaaatat ctgccggaaa gtccgaatca
    16981 gtacgccagc aaagaaaact cacaggaagc gcacgaagcg attcgtcctt ctgacgtcaa
    17041 tgtgatggcg gaatcgctga aggatatgga agcagatgcg cagaaactgt accagttaat
    17101 ctggcgtcag ttcgtagcct gccagatgac cccagcgaaa tatgactcca cgacgctgac
    17161 cgttggtgcg ggcgatttcc gcctgaaagc acgcggtcgt attttgcgtt ttgatggctg
    17221 gacaaaagtg atgcctgcgt tgcgtaaagg cgatgaagat cgcatcttac cagcagttaa
    17281 taaaggcgat gctctgacgc tcgttgaact tacaccagcc cagcacttta ccaagccgcc
    17341 agcccgtttc agtgaagcat cgctggttaa agagctggaa aaacgtggta tcggtcgtcc
    17401 gtctacctat gcgtcgatca tttcgaccat tcaggatcgt ggctacgtgc gagtagaaaa
    17461 tcgtcgtttc tatgcggaaa aaatgggcga aatcgtcacc gatcgccttg aagaaaattt
    17521 ccgcgagtta atgaactacg actttaccgc gcagatggaa aacagcctcg accaggtggc
    17581 aaatcacgaa gcagagtgga aagctgtact ggatcacttc ttctcggatt tcacccagca
    17641 gttagataaa gctgaaaaag atccggaaga gggtggtatg cgcccgaacc agatggttct
    17701 gaccagcatt gactgcccga cttgtggtcg caaaatgggg attcgcacag cgagcaccgg
    17761 ggtattcctt ggctgttctg gctatgcgct gccgccgaaa gagcgttgca aaaccaccat
    17821 taacctggtg ccggaaaacg aagtgctgaa cgtgctggaa ggcgaagatg ctgaaaccaa
    17881 cgcgctgcgc gcaaaacgtc gttgcccgaa atgcggcacg gcgatggaca gctatctcat
    17941 cgatccgaaa cgtaagttgc atgtctgtgg taataaccca acctgcgacg gttacgagat
    18001 cgaagagggc gaattccgca ttaaaggtta tgacggcccg atcgttgagt gtgaaaaatg
    18061 tggctctgaa atgcacctga aaatggggcg tttcggtaaa tacatggcct gcaccaacga
    18121 agagtgtaaa aacacacgta agattttacg taacggcgaa gtggcaccac cgaaagaaga
    18181 tccggtgcca ttacctgagc tgccgtgcga aaaatcagat gcttatttcg tgctgcgtga
    18241 cggtgctgcc ggtgtgttcc tggctgccaa cactttcccg aaatcgcgtg aaacgcgtgc
    18301 gccactggtg gaagagcttt atcgcttccg cgaccgtctg ccggaaaaac tgcgttatct
    18361 ggccgatgcg ccacagcagg atccggaagg taataagacc atggttcgct ttagccgtaa
    18421 aaccaaacag caatatgtct cttcggaaaa agacggaaag gcgactggct ggtcagcatt
    18481 ttatgttgat ggcaaatggg ttgaaggaaa aaaataacct ttaattctgt caggttttta
    18541 taaacaaagg gtcgcgaaag cggccctttt ttattgcata ttattttttc ttcacaccta
    18601 tacactaagg ctataaatga tatagtggtt atagttagca ccttttttat tattaaatcg
    18661 tattagtcac gccgcctggt gtgacgaaaa aacgatgttc tgatggcgtc taagtggatg
    18721 gtttaacatg aaattacaac aacttcgcta tattgttgag gtggtcaatc ataacctgaa
    18781 tgtctcatca acagcggaag gactttacac atcacaaccc gggatcagta aacaagtcag
    18841 aatgctggaa gacgagctag gcattcaaat tttttcccga agcggcaagc acctgacgca
    18901 ggtaacgcca gcaggacaag aaataattcg tatcgctcgc gaagtcctgt cgaaagtcga
    18961 tgccataaaa tcggttgccg gtgagcacac ctggccggat aaaggctcgc tgtatatcgc
    19021 caccacgcat acccaggcac gctacgcgtt accaaacgtt atcaaaggct ttattgagcg
    19081 ttatccccgc gtttctttgc atatgcacca gggctcgccg acacaaattg ctgatgccgt
    19141 ctctaaaggc aatgctgatt tcgctatcgc cacagaagcg ctgcatctgt atgaagattt
    19201 agtgatgtta ccgtgctacc actggaatcg ggctattgta gtcactccgg atcacccgct
    19261 ggcaggcaaa aaagccatta ccattgaaga actggcgcaa tatccgttgg tgacatatac
    19321 cttcggcttt accggacgtt cagaactgga tactgccttt aaccgcgcag ggttaacgcc
    19381 gcgtatcgtc ttcacggcaa cggatgctga cgtcattaaa acttacgtcc ggttagggct
    19441 gggggtaggg gtcattgcca gcatggcggt ggatccggtc gccgatcccg accttgtgcg
    19501 tgttgatgct cacgatatct tcagccacag tacaaccaaa attggttttc gccgtagtac
    19561 tttcttgcgc agttatatgt atgatttcat tcagcgtttt gcaccgcatt taacgcgtga
    19621 tgtcgttgat gcggctgtcg cattgcgctc taatgaagaa attgaggtca tgtttaaaga
    19681 tataaaactg ccggaaaaat aatttcttgc gttattttcg gcacctttta tgtagcgaag
    19741 gtgccggaat atattctctt ttgttactta tttctatacc gtg
//
LOCUS       DAZKIV010000084        19608 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZKIV010000084 DAZKIV010000000
VERSION     DAZKIV010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.084"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..106
                     /locus_tag="ACHOT6_002898"
     CDS             2..106
                     /locus_tag="ACHOT6_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001489687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFP9948080.1"
                     /translation="MLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            85..189
                     /gene="speFL"
                     /locus_tag="ACHOT6_002899"
     CDS             85..189
                     /gene="speFL"
                     /locus_tag="ACHOT6_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFP9948081.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            500..2698
                     /gene="speF"
                     /locus_tag="ACHOT6_002900"
     CDS             500..2698
                     /gene="speF"
                     /locus_tag="ACHOT6_002900"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFP9948082.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2695..4014
                     /gene="potE"
                     /locus_tag="ACHOT6_002901"
     CDS             2695..4014
                     /gene="potE"
                     /locus_tag="ACHOT6_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFP9948083.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIALATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCVIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4070..5710)
                     /gene="pgm"
                     /locus_tag="ACHOT6_002902"
     CDS             complement(4070..5710)
                     /gene="pgm"
                     /locus_tag="ACHOT6_002902"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFP9948084.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQQHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5736..6281)
                     /gene="seqA"
                     /locus_tag="ACHOT6_002903"
     CDS             complement(5736..6281)
                     /gene="seqA"
                     /locus_tag="ACHOT6_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFP9948085.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            6466..7230
                     /gene="ybfF"
                     /locus_tag="ACHOT6_002904"
     CDS             6466..7230
                     /gene="ybfF"
                     /locus_tag="ACHOT6_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9948086.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHALSPRDPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPRARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7370..7663
                     /gene="ybfE"
                     /locus_tag="ACHOT6_002905"
     CDS             7370..7663
                     /gene="ybfE"
                     /locus_tag="ACHOT6_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFP9948087.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7803..8333
                     /gene="fldA"
                     /locus_tag="ACHOT6_002906"
     CDS             7803..8333
                     /gene="fldA"
                     /locus_tag="ACHOT6_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFP9948088.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8543..8629
                     /gene="uof"
                     /locus_tag="ACHOT6_002907"
     CDS             8543..8629
                     /gene="uof"
                     /locus_tag="ACHOT6_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFP9948089.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8622..9068
                     /gene="fur"
                     /locus_tag="ACHOT6_002908"
     CDS             8622..9068
                     /gene="fur"
                     /locus_tag="ACHOT6_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFP9948090.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9153..9479)
                     /gene="chiQ"
                     /locus_tag="ACHOT6_002909"
     CDS             complement(9153..9479)
                     /gene="chiQ"
                     /locus_tag="ACHOT6_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFP9948091.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9529..10935)
                     /gene="chiP"
                     /locus_tag="ACHOT6_002910"
     CDS             complement(9529..10935)
                     /gene="chiP"
                     /locus_tag="ACHOT6_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFP9948092.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(11381..13045)
                     /gene="glnS"
                     /locus_tag="ACHOT6_002911"
     CDS             complement(11381..13045)
                     /gene="glnS"
                     /locus_tag="ACHOT6_002911"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFP9948093.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(13247..15193)
                     /gene="nagE"
                     /locus_tag="ACHOT6_002912"
     CDS             complement(13247..15193)
                     /gene="nagE"
                     /locus_tag="ACHOT6_002912"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFP9948094.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            15526..16326
                     /gene="nagB"
                     /locus_tag="ACHOT6_002913"
     CDS             15526..16326
                     /gene="nagB"
                     /locus_tag="ACHOT6_002913"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9948095.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            16386..17534
                     /gene="nagA"
                     /locus_tag="ACHOT6_002914"
     CDS             16386..17534
                     /gene="nagA"
                     /locus_tag="ACHOT6_002914"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9948096.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFLAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            17543..18763
                     /gene="nagC"
                     /locus_tag="ACHOT6_002915"
                     /gene_synonym="nagR"
     CDS             17543..18763
                     /gene="nagC"
                     /locus_tag="ACHOT6_002915"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFP9948097.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            18811..19563
                     /gene="nagD"
                     /locus_tag="ACHOT6_002916"
     CDS             18811..19563
                     /gene="nagD"
                     /locus_tag="ACHOT6_002916"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFP9948098.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         4774 a         4959 c         4948 g         4927 t
ORIGIN      
        1 tttgttgatc ttcttcacag attatagcca tttcatggat agaataactc taccttcaac
       61 tgacacagca agaggtaaag gtaaatggaa aataataacc gcttaatgcc tcatataagg
      121 cggacaaccc atatcatgaa gtttgcccat cgtaatagtt ttgactttca tttctttaat
      181 gcccgctagt cttctgacta aagggcaccc caacgtacag gtctccctga ctttaagcat
      241 tacaggttaa tacctgtatt cctcggtgct catatactgc taaccctttt aaactctaaa
      301 taattcgagt cgcagcactt gcaacttgag gtatgacgag tatagccagt taccgggctg
      361 gtctgggtta ttgcatctgc aaaaagcaaa ctactgattt atttatcagc ggtggagctt
      421 tgcttttttt ccggcgtgat cgatttctcc tttgagaaat tgaggacctg ctattacctg
      481 aaataaagag atgaataaaa tgtcagaatt aaaaattgcc gttagccgtt cttgcccgga
      541 ttgtttttcc actcatcgtg catgcgtgaa tattgacgaa agtaattata ttgacgttgc
      601 cgccattatt ttatcagtca atgatgttga acgtggaaaa ctcgatgaaa tagacgctac
      661 tggctatggc attcctgttt ttattgcaac ggaaaatgaa gaacgtgtac cagcagaata
      721 tctgccacgt atttctggtg tcttcgagca ttgcgaatca cggaaagagt tttatggacg
      781 tcagttagaa accgctgcta gccattatga aacgcaactg cgcccgccct tcttccgcgc
      841 actggtcgat tatgtcaatc agggcaatag cgcttttgac tgcccgggtc atcagggagg
      901 tgagtttttc cgtcgtcatc cggcagggaa tcagttcgtg gagtacttcg gagaaacgct
      961 gttccgttcc gacttatgca acgcagacgt agcaatgggc gatctgctga ttcacgaagg
     1021 cgcgccatgc attgcacagc aacatgcggc aaaagtgttt aatgccgata aaacatactt
     1081 cgttctaaat ggcacttcat cttctaacaa agtggtttta aacgccctgc taacaccggg
     1141 tgatctggtg ctgtttgatc gcaataacca caaatctaac caccacggag cgttgctaca
     1201 ggctggtgca acaccggttt atctggaaac ggcacgtaac ccgtatggct ttatcggtgg
     1261 tattgatgcg cactgttttg aagaaagtta cctgcgtgag ctgatcgcgg aagtcgcacc
     1321 gcagcgggca aaagaggctc gtcctttccg cctcgctgtg attcagttag gcacctacga
     1381 cggtacgatt tataacgccc gccaggtggt ggataaaatt ggtcatctgt gtgactacat
     1441 tctgtttgac tccgcatggg ttggctatga gcaatttatc ccgatgatgg ctgactgttc
     1501 gccgctgttg ctggatctta atgagaacga tccgggtatt ctggttacgc aatccgtaca
     1561 taaacagcag gctggttttt ctcaaacttc acaaatccat aaaaaagaca gccacatcaa
     1621 agggcaacag cgttatgtac cgcacaaacg catgaacaac gcctttatga tgcatgcctc
     1681 caccagcccg ttctatccgc tgtttgccgc actggatatc aacgccaaaa tgcatgaagg
     1741 tgtcagcggt cgtaatatgt ggatggattg tgtggtaaat ggcattaatg cccgcaagct
     1801 gatcctcgat aactgtcagc atattcgtcc gttcgtacct gaactggtgg atggtaaacc
     1861 ctggcagtcg tatgaaacag cgcaaattgc ggttgatctg cgcttcttcc agtttgtacc
     1921 aggtgagcac tggcattctt ttgaaggcta tgcagagaat caatactttg tcgatccatg
     1981 caaactgttg ctgacaaccc caggtattga tgcacgtaac ggcgaatatg aagcgttcgg
     2041 tgtacccgcg acgattcttg ctaacttcct gcgcgaaaat ggcgtagtgc cggaaaaatg
     2101 cgatcttaac tccatcctct tcctgctgac tccggcagaa gatatggcca aacttcagca
     2161 acttgttgcc ctgctggtac gcttcgaaaa actgcttgag tccgacgcgc cattagcaga
     2221 agtgctgcct tccatctaca aacagcatga agagcgctac gccggttata cccttcgtca
     2281 gttgtgccag gaaatgcatg atttgtatgc ccgtcacaac gtgaaacaac tgcaaaaaga
     2341 gatgttccgt aaggaacact tcccacgcgt aagcatgaat ccacaagaag ccaactacgc
     2401 ctatttacgc ggtgaagtgg agctggttcg tctgccggat gccgaaggcc gtatcgctgc
     2461 cgaaggtgcg cttccttatc ctccgggtgt gctgtgcgtt gttccgggtg aaatctgggg
     2521 tggtgctgtt ctgcgttact tcagcgctct ggaagaaggg atcaacctgt tgccaggttt
     2581 tgcaccggag cttcagggtg tctatatcga agaacatgat ggtcgtaagc aagtttggtg
     2641 ctatgtcatc aagcctcgtg atgcgcaaag caccctgttg aaaggggaaa aattatgagt
     2701 caggctaaat cgaacaaaat gggcgtcgtt cagttaacca tactgacgat ggtcaacatg
     2761 atgggctctg gcatcatcat gctgccgaca aagcttgccg aagtcgggac aatctcaatt
     2821 atctcctggc tggtgacagc cgtaggctca atggcactgg catgggcatt cgcaaaatgc
     2881 ggtatgttca gccgtaaatc aggcggtatg ggcggttatg cggagtatgc tttcggtaaa
     2941 tccgggaact ttatggcgaa ctacacctac ggtgtctcgc tgctgattgc taacgtcgcg
     3001 attgctattt cggcggttgg ttacggcacc gaactgctcg gcgcgagttt gtcaccagtg
     3061 cagattgctc ttgcgaccat cggggtgctg tggatttgta ccgtggctaa ctttggtggt
     3121 gcgcgcatta ccgggcaaat cagtagcatt accgtgtggg gggtcattat tccggtggta
     3181 ggcctgtgcg ttatcggctg gttctggttt agcccgacgc tttacgttga ttcctggaat
     3241 ccgcatcatg caccgttctt cagtgcggta ggttcttcca tcgctatgac gctatgggct
     3301 tttcttggtc tggagtctgc gtgtgcgaat actgatgtag tggaaaaccc ggaacgtaat
     3361 gtgccaatcg cggtactcgg cggtacgtta ggtgcggcgg tgatttatat cgtctccacc
     3421 aacgtgattg ccgggattgt gccaaatatg gagctggcaa attcaacggc accatttggt
     3481 ctggccttcg cgcagatgtt cacgccggaa gtgggtaaag tcattatggc gctgatggtg
     3541 atgtcctgct gcggttcgct gcttggctgg cagttcacca ttgcccaggt gtttaaatct
     3601 tcatctgatg aaggctactt ccctaaaatt ttctcccgtg taaccaaagt ggatgcaccg
     3661 gttcagggaa tgttgaccat tgtgattatt cagagtggat tggcactgat gaccattagc
     3721 ccgtcactga acagccagtt caacgtgctg gttaacctgg ccgtggtgac caatatcatc
     3781 ccgtatattc tgtcgatggc ggcattagtc attattcaga aggtcgctaa tgtgcccccc
     3841 tcaaaagcga aagttgcaaa ctttgttgct tttgttggcg cgatgtatag cttttatgcg
     3901 ctgtactcat ccggggaaga agccatgctg tacggttcca tcgtgacctt ccttggttgg
     3961 acactgtatg gtctggtatc accacgcttt gaactgaaaa ataaacacgg ttaaaaaagc
     4021 aaagtaaaaa agggcgatct tgcgaccgcc ctttttttat taaatgtgtt tacgcgtttt
     4081 tcagaacttc gctaacaatc tcaaccgctt ctttctcaat ctgcttgcga tgttcttcac
     4141 cgaggaagct ttcgcagtag atcttatatg cgtcttccgt gcctgacgga cgcgcggcga
     4201 accagccgtt gtcagtcatc actttcagac cgccaataga agcaccgttg cccggagcag
     4261 cagtcaggcg cgcggtgatc gggtcacctg ccagggtgct ggcgctcacc atttccggag
     4321 acagcttaga cagcgccgct ttttgtgcgg aagtcgcagc tgcctgcaaa cggttgtagc
     4381 tcggcgcacc aaagcgtttt gccagttcgt tgtagtgttg ctgcgggttc ttaccggtga
     4441 cagcggtgat ttccgccgcc agcagacaca taatgatgcc gtctttgtcg gtggaccacg
     4501 gcgtgccgtc gaaacgcagg aaggaagccc ctgcgctctc ttcgccgcca aagccgaagc
     4561 tgccgtcgaa cagaccatca acaaaccatt tgaaacctac cggtacttct accagcttac
     4621 ggcccaagtc attgaccaca cggtcgatca tcgcagaaga aaccagcgtt ttaccgacag
     4681 caacatcttt gccccactgc ggacgatgct ggaacaggta gttgatcgcc accgccaggt
     4741 agtggttcgg attcatcaaa cctgccggag tgacgatacc gtgacggtca taatccgggt
     4801 cgttagcaaa cgccagatcg aacttatcac gcagtgccag cagacccgcc atcgcacact
     4861 cggaggagca gtccatacgg atcgcgccgt ctttatcaag gtgcataaag cggaaggttt
     4921 gatcgacctg atcgttaacg atggtcaggt tgaggttgta atactcgcca atacgcttcc
     4981 agtattcgat accggaaccg cccagcggat caacgcccag cgtcaggccc gctttctgaa
     5041 ttgccgccat atcaacgata tcggccagac cttccacaaa cggctgcacc agatcctgct
     5101 cttttacatg accggatgcc atcgcttcgt cgagggagat acgcttcacg cctttcaggc
     5161 catcggccat cagtgcgttg gccctgtctt ccaccacttt ggtgacgttg gtatcagccg
     5221 ggccaccatt tggcgggttg tacttgatac caccatcttc cggcgggtta tgggacggtg
     5281 taatcacgat accgtctgcc agcgggccac cttttttatt gtgaaccagg atggcattgg
     5341 aaatggcagg tgtcggagtg aagccattgt tttcctgcac aatgacatca acgccgttcg
     5401 ctgccagcac ttccagtacg gaaatgaacg caggctcgga cagggcgtga gtatctttac
     5461 ccacatagca agggccagtg atgccgtttt tcgcacgttc ttcagcaatt gcctgagcga
     5521 tcgccagaat gtgcggctcg ttaaagctgt ggcgcgctgc actgccacgg tgaccggaag
     5581 taccgaattt caccgcgtgc tccgcattcc ctgcttctgg tttcagtaca taatattgcg
     5641 ccgtcagttg ggcgacgtta atcaaatcac tctgttgtgc aggttggcct gcacgattgt
     5701 ggattgccat tgctttgtcc tttgtctgca acgttttaga tagttccgca aaccttctca
     5761 atcaattccg ccgggaattg catcgactgc atgatgtgtt cgatcatgct gcatttacgg
     5821 ccggtgttgg tgttggtgat cacccaatac ggcgtgcctg gcacatgttt cggcttggtc
     5881 tgattaccat ttttcagcag cgtttgttca tctgccgcaa agtaaacgcg tgtacgaccg
     5941 tgcaacgatt ccgttgcttc ggcaaacgcc tgggcgtcaa gagaatatag tgtagacaac
     6001 agcagcataa agcgattgac cgctcgcttt tgctctgcgt attcatccga aagcagaagt
     6061 tcacgcattg cgcgaacctt gtctttaatc gttttgaccg gcttcgcttc gacgatagca
     6121 ggtgacgcaa cgcgaacctc tttcgtcacc ggagcagcag gctgtgatgc ggcggaaaat
     6181 ttcaacatac gccgtaaaat gtcggatgcg ctctcgccga tatgcttagt gtggctggca
     6241 atatagctgt agagttcatc atcaacttca atcgttttca tcttaatcca gtgcagtgtc
     6301 ttagctgaat acaagtcgcc aggagtatat gaataaatcc cggcagcgga tagcgtcaag
     6361 cctggcttgc agaaaaactc ggaataaacc ttatttgttg cagccctgtg gcacaatggt
     6421 caacataata ccctaacccg acaacgcgaa aaaaagaact ttgccatgaa attgaatatc
     6481 cgcgcgcaaa ctgcacaaaa ccagcacaat aattctccca tcgttcttgt ccatggtctg
     6541 tttggtagcc tcgacaacct tggcgtactg gctcgcgatc tggtaaacga tcacaatatc
     6601 atccaggttg atatgcgtaa ccacgctctt tcaccgagag atccggtaat gaattacccg
     6661 gcgatggcgc aggatcttgt tgataccctg gatgcactgc agatcgacaa agcaacattt
     6721 atcggtcact ccatgggcgg taaagcggta atggcactta ctgcactggc ccccgatcgg
     6781 attgacaagc tggtggcaat tgatattgct ccggtcgatt atcacgttcg ccggcatgat
     6841 gagattttcg cggccatcaa cgcggtcagt gaatcggacg cacaaactcg ccagcaagca
     6901 gcagcaataa tgcgccagca tcttaatgaa gaaggggtga ttcagtttct gttgaaatct
     6961 tttgttgacg gggagtggcg ctttaacgtg ccggtattgt gggatcagta tccgcatatt
     7021 gtgggttggg agaaaatccc ggcatgggat caccctgccc tgtttatccc tggcggcaat
     7081 tctccgtatg ttagcgagca gtaccgtgat gatttactgg cacaatttcc acgggcacgg
     7141 gcgcatgtga ttgcaggcgc gggtcactgg gtccatgctg aaaaaccgga tgcggtatta
     7201 cgcgctatcc gtcgctatct caatgattaa cgcaactgat taaaaaccca gcgtccgcgt
     7261 cgctttccgc gcgtgggcgt tggcgtgact catcgcgctg atgtattatg gcgcgctatc
     7321 catccgggca gaggcctggc tgattgtttc ccccgaagtc accaagatca tggccaaaga
     7381 acaaacggac cgtacgacat tagatctgtt cgcgcacgag cgtcgaccgg gacgaccgaa
     7441 aactaatccg ctttcgcgcg atgaacagct gcgtattaat aaacgcaacc agctaaaacg
     7501 cgacaaagta cgtggcctta agcgtgtcga actgaagctg aacgcggaag ctgtcgaggc
     7561 gctgaacgag ctggcggaat cgcgcaatat gagccgtagc gaactgatcg aagagatgtt
     7621 aatgcagcaa ctggcggcgc tgcgtagtca gggcatcgtt taaatttcca ctttcatgta
     7681 gcacagtgtg cagtcctgct cgtttgctga ttccgcaaga ctgcctgttc tgctatgatt
     7741 gcctttatcc gtgggcaatt ttccaccccc atttcaataa gtttcaagag gttatttcac
     7801 tcatggctat cactggcatc tttttcggca gcgacaccgg taataccgaa aatatcgcaa
     7861 aaatgattca aaaacagctt ggtaaagacg ttgccgatgt ccatgacatt gcaaaaagca
     7921 gcaaagaaga tctggaagct tatgacattc tgctgctggg catcccaacc tggtattacg
     7981 gcgaagcgca gtgtgactgg gatgacttct tcccgactct cgaagagatt gatttcaatg
     8041 gcaaactggt tgcgctgttt ggttgtggtg accaggaaga ttacgccgaa tatttctgcg
     8101 acgcattggg caccatccgc gacatcattg aaccgcgcgg tgcaaccatc gttggtcact
     8161 ggccaactgc gggctatcat ttcgaagcat caaaaggtct ggcagatgac gaccactttg
     8221 tcggtctggc tatcgacgaa gaccgtcagc cggaactgac cgctgaacgt gtagaaaaat
     8281 gggttaaaca gatttctgaa gagttgcatc tcgacgaaat tcttaatgcc tgatgtgatg
     8341 cggcgtagac tcatgtctac gccgtattaa tagataatgc caatcaaaat aattgctaca
     8401 aatttgtaac ttttgctgtt gtacctgtac aatgtcccgg tgttcaagtg gccttgccgt
     8461 tgtaaatgta agctgtgcca cgtttttatt aacaatattt gccagggact tgtggttttc
     8521 atttaggcgt ggcaattcta taatgatacg cattatctca agagcaaatt ctgtcacttc
     8581 ttctaatgaa gtgaaccgct tagtaacagg acagattccg catgactgat aacaataccg
     8641 ccctaaagaa agctggcctg aaagtaacgc ttcctcgttt aaaaatcctg gaagttcttc
     8701 aggagccgga caaccatcac gtcagtgcgg aagatttata caaacgtctg atcgatatgg
     8761 gtgaagaaat tggtctggct acggtatatc gcgtactgaa ccagtttgac gacgctggta
     8821 tcgtcacccg ccacaatttt gaaggcggta aatccgtatt tgaactgaca cagcaacatc
     8881 accacgatca cctgatctgc ctcgactgcg gcaaggttat cgaatttagt gatgattcca
     8941 tcgaagcgcg tcagcgtgaa attgccgcaa aacatggcat tcgcctgact aaccacagtc
     9001 tctatcttta cggtcactgt gccgaaggcg attgccgcga agatgaacat gcgcacgaag
     9061 gcaaataagc cagcctgaat gagaaaaagc caacctgcgg gttggctttt ttatgcaagg
     9121 gaaataagaa aagcgagagt tacagctctc acttatttgt tattactgcg aatttcctgc
     9181 caaactttat cacaatcggc tttcaccgcc tgatcatttc cggtttgcgt tgcacgcaga
     9241 cactgctgat aatccagtac gcgtacactt tcctggtcgg caaactgctg atgttgtttc
     9301 tctttcttca gcacgtttaa cacgctctgg caggcttcaa ttttttctgg cgaaccttgc
     9361 gcggtgttga tacaggcgct gtatgcctct ttcagacggc tgtcttcctg tggtgcggtt
     9421 gattgcgcac aagctaccag ccccgatgcc atcatggcga tgagaatcag ttttttcatt
     9481 gaaaattcct caaacgtgcg gcaggcacaa aacctgccgc gtcgggcatc agaagatggt
     9541 gaatggtgcg attaccataa atttcacgtc acgctcatcc tggaagatgt taccgtaacc
     9601 gccgccccag cttgggatgt cggagtggtt gtcgtattcg gtgaagtgca gtttgaacat
     9661 cgtgcctttg gcgcgaccgt cctgaatggt gtagaccgca tccaggctgt atgcagactc
     9721 ttcaatagtc cggtttttgt cgtagtacgc atccggattg ctctgccagg tcgcaggttt
     9781 agcatcccat gcgtaaacgt aggaagcgcc gatggcgaag cctggaagat tccagttttt
     9841 caggtcatac atcgcaccga agaagaccgc tttttcgccg ttagcgttga agtcggaacg
     9901 gttatcccac cagatatcca ggcggccgtt ggaggaagcg taggttggag tcatacgttg
     9961 caggaagtat ccctgctgac cgtcagcctt aacccaggtg ccttcgaggc gcaaatccac
    10021 tacgtcagcc gcccggtaac caaaggtcaa cgcttgcagc caggcggtgc cgtcataaag
    10081 gtcgttgacg ctgcgatcgt caactttatc gcgcgtacca tagaactggt agctggtggt
    10141 taacgggcta ccggcgatat caaatttgta gctggctttg gcaaaatatt gatcgatata
    10201 cccttccgcc tgaccaaatg ccgcttccag tacgaagtta tttttgaagt cgtatttcgc
    10261 cccaagggag tgcagataat caactttggt ggttttatcg ttctgataaa actcatccat
    10321 ttccagatgc cacggcgctt tgtattcgtt ggtccacatg taggagaaac tcaaggcacc
    10381 agcatcgccg taatcaaaat tcgccccggc ttccgccccc tgataagtac ctggcataaa
    10441 gctccagtgc ggcgctaaca gcgtttgacc agttggctga atgtaacctg ccctcgccca
    10501 aaccggaccg tatttaaatt tggccgcagc tttatacagg cttataccgc ttttgtcgcc
    10561 ggaccagtct tcgtcatagg ctttattact ttttgaaaac gcgatttcgt tcgggtggga
    10621 gctgtcgccg ttttccgcca tttcaatcgc cgtaaacgca gcaatatcaa ggccgaacat
    10681 atcagcagca taaccggact gaaaatcgag gttggcattc caggtggagt gagaaaggtt
    10741 ggttttgtat ttgtcgccgt cggtaacatc tttacggtcg cgttcacgct gccagtaata
    10801 tataccgccg gttaaggttg aatcgtcgat gaatccttcg gcgcgagcct ccggcattgc
    10861 cataaagccc gacattgctg taacaccggc gatagccagc gccagcgtac tacgtttgcc
    10921 actaaacgta cgcatgggtt aatcctcttt gacgtataaa ttgctgcacc aaaggtgaac
    10981 agcgaaaaaa taaaaattaa aaaagtagcg ttgagaaaag tctcgctgac tacaggaaca
    11041 gactattttt cgcgacgcga attatcaatc tctttctgta accagaatgt aagaacatga
    11101 gcaaccgcac atattttgat gatttttgtt acaaggctta agtttgcggt tgaaaggctt
    11161 gcagaagaat aaaagcgtga atttccagca agatgaaagc ggttacattt tatttaatta
    11221 tggtttgtga ttttaaattt attttatgga taaattgcga agcggaatta attcgcgaag
    11281 aaaagcagaa aaaacgccgc agagtgcggc gtttgggatt gtttgctatt tcttgccgga
    11341 tgcgacgtaa atgcctcacc cggcctggta gaacttaaaa ttactcgcct actttcgccc
    11401 aggtatcacg cagcccaacg gtgcggttaa ataccggttt ttccgctgtg gaatggcggc
    11461 tgtcgaggca gaagtaacct tcacgctcaa actggaatgc tttacccgca accgcatctt
    11521 tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga ttccgggtta atcaccgaca
    11581 ggaaatcatc cgcagcacca gggttcggca cgctgaacag acggtcgtac agacggattt
    11641 caaccggcag cgcatgtgcc gcgctcaccc agtgaataac acctttgact ttacgaccat
    11701 ctgccggatc tttgcttaag gtgtcggcgt cataagtaca gaagatggtg gtgatattgc
    11761 cttcggcatc tttctccacg cgttcagcct taatgacgta agcattacgc agacgcactt
    11821 ctttacccag caccagacgt ttgtactgct tgttagcttc ttcgcggaaa tcggcgcgat
    11881 cgatccaaat ctcaccgcta aacggcacct gacgactccc catttccggt ttgttcggat
    11941 ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt ttcgataacc agtttcaccg
    12001 gatcgataac cgccattgcg cgcggcgcat tttcgttgag atcttcacgg atgcaggatt
    12061 ccagcgatgc catctcaatg gtgttgtcct gcttggtcac gccgatgcgt ttgcagaact
    12121 cacgaataga agcagcagtg taaccacgac gacgcagacc ggaaatggtc ggcatacgcg
    12181 ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag gttcagctta cgcttggaca
    12241 tcacagtgta ttccagattc aggcgcgaga attcgtactg gcgcgggtga acaggaatgg
    12301 tgatgttgtc cagcacccag tcgtacaggc gacggttgtc ctggaactca agcgtacaca
    12361 gagagtgcgt aataccttcc agggcatcgc tgatgcagtg ggtgaagtcg tacatcgggt
    12421 agatgcacca cttgttgcca gtctggtggt gttcagcaaa tttaatacgg tacagcaccg
    12481 gatcgcgcat cacgataaac ggtgaagcca tgtcgatttt cgcacgcagg caggctttac
    12541 cttcttcaaa accaccggca cgcatttttt cgaacagcgc caggttctct tcaacgctgc
    12601 gatcgcggta cgggctgttt ttacccggtt gcgtcagggt gccgcggtat tcgcggatct
    12661 gttccggcgt cagttcatca acgtacgcca ggcctttatt gatcagttcg atcgcatagg
    12721 cgtggagctg atcaaaataa tcggaggagt aacggacgtt accagaccag tgaaaaccta
    12781 accactctac gtcgtttttg atcgactcaa catactcgat atcttctttt accgggttag
    12841 tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg ggcgatcccg aagttcaggc
    12901 agatagattt cgcatggcca atatgcagat agccattcgg ctccggcggg aaacgggtgt
    12961 gtactgtggt gtgcttacca ctggccagat cttcatcgat gatctgacgg ataaagttag
    13021 tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa gcgtaaacaa cgtataacgg
    13081 cgtatgatct tataagcggg acaggctgac aactgttagt tttttaaaat tgataatcta
    13141 acaaggatta tggggatgat tgatgaaaaa aaagcggcag agaagatctc caccgcttgt
    13201 cattgttgga tgcgacgctc aagcgtcgca tcaggcataa agcagattac tttttgattt
    13261 catacagcgg tgtttgaccc gccacaacat ggccctgagc tttaatgatc aagccactga
    13321 aatcgtcgat attgctgcaa accaccgggc taatcatcga gcgggcgtta gcgttcaggt
    13381 aatccagatc catttccaga atcggttgcc ctgcgcttac ctgcgcaccc tcttccacca
    13441 gacgtttaaa gcctttacct tccagcgcta cggtgtcgat acccatatgg acgacgatct
    13501 ccgcgccttt ttcggtttcc aggcagaacg cgtggttggt gttgaagatt ttcacgattg
    13561 tccctgcggc tggtgatacg acgattttat ctgtcggttt caccgccaca ccgtcaccca
    13621 ccgctttgct ggcgaatgct tcgtcaggaa cctgatccag tgccacgaca tcaccggtaa
    13681 tcggcgatac cagctccgcg atagataccg cgtttggtac agcctgcggt tttgctacag
    13741 gcgcggcagt tgccggagtt gcttcagctg acgcagcggc taccggacca cgggcaacga
    13801 ctttcttcat cgcatcgccg atggattctg ctttcgcgcc aacaatcacc tgaatagtct
    13861 gtttgttcag tttcactaca ccagaagcac ccagacgttt acacatcgta tcgttgacgc
    13921 gggcagagtc agccacggtc aggcgcagac gggtgataca ggcgtcaatt gctttcaggt
    13981 tgtcagtgcc gccaaccgca gcaatatagt tggtcgccag ttgattgagg ccttcttcag
    14041 tgttgctgtt ggcttcttca gtaacgatct cgtcttcttt atcttcacga cccggcgttt
    14101 tcaggttgaa catgcggata accaaactga acaccacgaa gtagatagcg aagaagacaa
    14161 cacccataac cagcagcatc cagacgttct ggctggcggc cggcaggtta tacatcaacg
    14221 cgtagtcgat agcccccgca gagaaagaga agccagcatg gatacccagc agtgttgcca
    14281 caaacaggct gataccggtc agcagtgcgt gcaggaggta cagcagcggt gcaaggaaca
    14341 tgaacaggaa ttccagcggc tcagtcacac cggtcaggaa cgcagtaaca gcaacagaaa
    14401 gcagcatccc gccaaccatc ggacgacgct ctttcggtgc tgcgaagtac atcgccagcg
    14461 ccgcacccgg cagaccgaac atcataatcg ggaagaagcc ggacatgaac atccccgcgg
    14521 tgccgtcacc ggcatagaag cggttgatat caccgtggaa aaccgtaccc gccgcgttgg
    14581 tgaattcacc aatctggaac caggcgatgg tgttcagcac ctgatgcaga ccggttggga
    14641 tcagcagacg gttgatgaaa ccaaagatac cggaacccag cgcgcccgca gaaacgatcc
    14701 actcgcctcc tgcatggata gcgtgctgta ccggcggcca gacgtaacca aaaatggccg
    14761 ccagcaccag gcagaagaag ccggtggcaa tcggcacaaa gcgtttgccg ccgaagaaac
    14821 tcaggaagtc cggcagttta atatcggacc aacggttata ggctgcgcca ccaaccagac
    14881 cggtaatgat acccgccagt acacccatgt taatttctgg gttgatggtc accatcgctt
    14941 tggttaacac aaagtaacct accgcacccg ccagcgccgc cgcacctgcg ctgtctttcg
    15001 accagctgga tgccacaccg atggcgaaga ttaatgcgag gttatcaaaa atcgcaccgc
    15061 ccgcctgggc aataaacgca acgttaagta aatctggctg accgaatcgc agcaacagtg
    15121 ccgccaccgg cagcaccgcg atagggagct gtaacgccct accgagtcgc tggaaaaaac
    15181 ctaaaatatt catcttattc cccctacgag aaccctattt ggctcgttta aagccgtatt
    15241 tttattttgc tgcaaattgt actgccgatg ttctgtaatc agattgttag atcatctgct
    15301 acagagtgtg tgaaaattta attcgtatcg caaattaaac gcgtgtcttt tgtgagtttt
    15361 gtcaccaaat atcgttatca tcactccctt ttactggcta aagcagaaaa cttattttat
    15421 cattcaaaaa atcaggtcgg attgacgcct gtctgcgcaa atccaggtta cgcttaaaga
    15481 tgcctaatcc gccaacggct tacattttac ttattgaggt gaataatgag actgatcccc
    15541 ctgactaccg ctgaacaggt cggcaaatgg gctgctcgcc atatcgtcaa tcgtatcaat
    15601 gcgttcaaac cgactgccga tcgtccgttt gttctgggcc tgccgactgg cggcacgccg
    15661 atgaccacct ataaagcgtt agtcgaaatg cataaagcag gccaggtcag ctttaagcac
    15721 gttgtcacct tcaacatgga cgaatatgtc ggtctgccga aagagcatcc ggaaagctac
    15781 tacagcttta tgcaccgtaa tttcttcgat cacgttgata ttccagcaga aaacatcaac
    15841 cttctcaacg gcaacgcccc ggatatcgac gccgaatgcc gccagtatga agaaaaaatc
    15901 cgttcttacg gaaaaattca tctgtttatg ggcggtgtag gtaacgacgg tcatattgca
    15961 tttaacgaac cggcgtcttc tctggcttct cgtactcgta tcaaaacctt gactcatgac
    16021 actcgcgtcg cgaactctcg tttctttgat aacgatgtca atcaggtgcc aaaatatgcc
    16081 ctgactgtcg gtgttggtac attgctggat gccgaagaag tgatgattct ggtgctgggt
    16141 agccagaaag cactggcact gcaggctgca gttgaaggtt gcgtgaacca catgtggacc
    16201 atcagctgtc tgcaactgca tccaaaagcg atcatggtgt gcgatgaacc ttccaccatg
    16261 gagctgaaag ttaagacttt aagatatttc aatgaattag aagcagaaaa tatcaaaggt
    16321 ctgtaattgt tatccctgcc ctggctcctt gctcagggca atatttttta aaatcggggg
    16381 tcagaatgta tgcattaacc cagggccgga tctttaccgg ccacgaattt cttgatgacc
    16441 acgcggttgt tatcgctgat ggcctgatta aaagcgtctg tccggtagcg gaactgccgc
    16501 cagagatcga acaacgttca ctgaacgggg ccattctctc ccccggtttt atcgatgtgc
    16561 agttaaacgg ctgcggcggc gtacagttta acgacaccgc tgaagcggtc agcgtggaaa
    16621 cgctggaaat catgcagaaa gccaatgaga aatcaggctg tactaactat ctgccgacgc
    16681 ttatcaccac cagcgatgag ctgatgaaac agggcgtgcg cgttatgcgc gagtacctgg
    16741 caaaacatcc gaatcaggcg ttaggtctgc atctggaagg tccgtggctg aatctggtaa
    16801 aaaaaggcac ccataatccg aattttgtgc gtaagcctga tgccgcgctg gtcgatttcc
    16861 tgtgtgaaaa cgccgacgtc attaccaaag tgaccctggc accggaaatg gttcctgcgg
    16921 aagtcatcag caaactggca aatgccggga ttgtggtttc tgccggtcac tccaacgcga
    16981 cgttgaaaga agcgaaagcc ggtttcctcg cggggattac ctttgccacc catctgtaca
    17041 acgcgatgcc gtatattacc ggtcgtgaac cgggcctggc gggcgcgatc ctcgacgaag
    17101 ctgacattta ttgcggtatt atcgctgatg gcctgcatgt tgattacgcc aacattcgta
    17161 acgctaaacg ccttaaaggc gacaaactgt gtctggttac cgacgccacc gcgccagcag
    17221 gtgccaacat tgaacagttc atttttgcgg gtaaaacaat atactaccgt aacggacttt
    17281 gtgtggatga gaacggtacg ttaagcggtt catccttaac catgattgaa ggcgtgcgta
    17341 atctggtcga acattgcggt atcgcactgg atgaagtgct gcgtatggcg acgctctatc
    17401 cggcgcgtgc gattggcgtt gagaaacgtc tcggcacact cgccgcaggt aaagtagcca
    17461 acctgaccgc attcacacct gattttaaaa tcaccaagac catcgttaac ggtaacgagg
    17521 tcgtaactca ataagagaaa gtatgacacc aggcggacaa gctcagatag gtaatgttga
    17581 tctcgtaaaa cagcttaaca gcgcggcggt ttatcgcctg attgaccagt acgggccaat
    17641 ctcgcggatt cagattgccg agcaaagcca gcttgccccc gccagcgtaa ccaaaattac
    17701 gcgtcagctt atcgaacgcg ggctgatcaa agaagttgat cagcaggcct ccaccggggg
    17761 ccgccgcgct atctccatcg tcaccgaaac ccgcaatttc cacgcaatcg gcgtacggct
    17821 tggtcgtcac gacgccacca tcaccctgtt tgatctcagc agcaaagtgc tggcagaaga
    17881 acattacccg ctgccggaac gtacccagca aacgctggaa catgccctgt tgaatgccat
    17941 tgctcagttt attgatagct accagcgcaa actgcgcgag ctgatcgcga tttcggtgat
    18001 cctgccaggg cttgttgacc cggacagcgg caaaattcat tacatgccgc atattcaggt
    18061 agaaaactgg gggctggtag aagctctgga agaacgtttt aaagtgacct gtttcgttgg
    18121 tcacgatatc cgtagtctgg cgctggcgga gcactacttc ggtgcaagtc aggattgcga
    18181 agactccatt ctggtgcgtg tccatcgcgg aaccggggcc gggattatct ctaacgggcg
    18241 catttttatt ggccgcaacg gtaacgtcgg tgaaattggc catattcagg tcgaaccgct
    18301 gggtgaacgc tgccactgcg gcaactttgg ctgcctggaa actatcgccg ccaacgctgc
    18361 cattgaacaa cgggtgttga atctgttaaa gcagggctac cagagccgcg tgccgctgga
    18421 cgactgcacc atcaaaacta tctgcaaagc cgcgaacaaa ggcgatagtc tggcgtcgga
    18481 agtaattgag tatgtcggtc gtcatctggg taaaaccatc gccattgcta tcaacttatt
    18541 taatccgcaa aaaattgtta ttgccggtga aatcaccgaa gccgataaag tgctgctccc
    18601 tgctattgaa agctgcatta atacccaggc gctgaaggcg tttcgcacta atctgccggt
    18661 ggtacgttct gagctggatc accgctcggc aatcggcgct tttgcgctgg taaaacgcgc
    18721 catgctcaac ggtattttgc tccagcattt gctggaaaat taatgtgctt ttatagtggc
    18781 gcttattgtt gtcaatattc tgggtagtcc atgaccatta aaaatgtaat ttgcgatatc
    18841 gacggcgtgc tgatgcacga taacgtcgcc gtaccgggtg cagcggaatt tttgcacggg
    18901 attatggata aaggcctgcc gctggtgttg ctgaccaact atccttcgca gaccgggcaa
    18961 gatctggcga accgctttgc cactgccggt gtcgatgtgc cggatagcgt gttttatacc
    19021 tctgcgatgg cgaccgccga tttcctgcgt cgccaggaag gcaagaaagc atatgtggtg
    19081 ggcgaaggcg cactgattca tgaactgtac aaagccggtt tcactattac cgatgtgaac
    19141 cctgatttcg tgattgttgg cgaaacgcgt tcctacaact gggacatgat gcataaagcg
    19201 gcctatttcg tcgctaacgg tgcacgcttt atcgccacca acccggacac ccacgggcgc
    19261 ggtttttatc ccgcttgtgg cgcgttgtgt gcagggattg agaaaatctc cgggcgcaaa
    19321 ccgttctatg ttggtaagcc cagcccgtgg atcatccgcg cagcattaaa caaaatgcag
    19381 gcgcattcgg aagaaacggt gattgtcggc gataacttgc gcaccgacat tctggcgggc
    19441 ttccaggcag gtctggaaac gattctggtg ctttctggtg tttcatcgct cgacgatatc
    19501 gacagtatgc cgttccgccc aagctggatt tacccgtcag tcgctgaaat cgacgttatc
    19561 tgaataccac ccacgtctgc ggacgtggtt ggctctgatg acaaacgc
//
LOCUS       DAZKIV010000085        19108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZKIV010000085 DAZKIV010000000
VERSION     DAZKIV010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.085"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..1226
                     /gene="torY"
                     /locus_tag="ACHOT6_002917"
     CDS             126..1226
                     /gene="torY"
                     /locus_tag="ACHOT6_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFP9948099.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEGGIKELTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1251..3680
                     /gene="torZ"
                     /locus_tag="ACHOT6_002918"
     CDS             1251..3680
                     /gene="torZ"
                     /locus_tag="ACHOT6_002918"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFP9948100.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLLITTS
                     FERNDLTMTGDYSNQNIVPMKQAVAPQFEARNDFDVFAELAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAIADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAVQA"
     gene            complement(3845..4816)
                     /gene="cmoB"
                     /locus_tag="ACHOT6_002919"
     CDS             complement(3845..4816)
                     /gene="cmoB"
                     /locus_tag="ACHOT6_002919"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFP9948101.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSAGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIADVS
                     VTTTEEQRRTEWMVTESLADFLDPHDPGKTVEGYPAPKRAVLIARKP"
     gene            complement(4813..5556)
                     /gene="cmoA"
                     /locus_tag="ACHOT6_002920"
     CDS             complement(4813..5556)
                     /gene="cmoA"
                     /locus_tag="ACHOT6_002920"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFP9948102.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYHGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HNAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(5597..5992)
                     /gene="yecN"
                     /locus_tag="ACHOT6_002921"
     CDS             complement(5597..5992)
                     /gene="yecN"
                     /locus_tag="ACHOT6_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFP9948103.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            complement(6045..6863)
                     /gene="yecE"
                     /locus_tag="ACHOT6_002922"
     CDS             complement(6045..6863)
                     /gene="yecE"
                     /locus_tag="ACHOT6_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFP9948104.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            complement(6860..7426)
                     /gene="yecD"
                     /locus_tag="ACHOT6_002923"
     CDS             complement(6860..7426)
                     /gene="yecD"
                     /locus_tag="ACHOT6_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFP9948105.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPFPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            7736..9508
                     /gene="aspS"
                     /locus_tag="ACHOT6_002924"
     CDS             7736..9508
                     /gene="aspS"
                     /locus_tag="ACHOT6_002924"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFP9948106.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            9626..10078
                     /gene="nudB"
                     /locus_tag="ACHOT6_002925"
     CDS             9626..10078
                     /gene="nudB"
                     /locus_tag="ACHOT6_002925"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFP9948107.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            10107..10847
                     /gene="yebC"
                     /locus_tag="ACHOT6_002926"
     CDS             10107..10847
                     /gene="yebC"
                     /locus_tag="ACHOT6_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9948108.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            10882..11403
                     /gene="ruvC"
                     /locus_tag="ACHOT6_002927"
     CDS             10882..11403
                     /gene="ruvC"
                     /locus_tag="ACHOT6_002927"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFP9948109.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(11405..12007)
                     /gene="yebB"
                     /locus_tag="ACHOT6_002928"
     CDS             complement(11405..12007)
                     /gene="yebB"
                     /locus_tag="ACHOT6_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HFP9948110.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            12078..12143
                     /gene="yobI"
                     /locus_tag="ACHOT6_002929"
     CDS             12078..12143
                     /gene="yobI"
                     /locus_tag="ACHOT6_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HFP9948111.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            12282..12893
                     /gene="ruvA"
                     /locus_tag="ACHOT6_002930"
     CDS             12282..12893
                     /gene="ruvA"
                     /locus_tag="ACHOT6_002930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFP9948112.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPVSPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            12902..13912
                     /gene="ruvB"
                     /locus_tag="ACHOT6_002931"
     CDS             12902..13912
                     /gene="ruvB"
                     /locus_tag="ACHOT6_002931"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFP9948113.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(14059..14844)
                     /gene="znuB"
                     /locus_tag="ACHOT6_002932"
     CDS             complement(14059..14844)
                     /gene="znuB"
                     /locus_tag="ACHOT6_002932"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFP9948114.1"
                     /translation="MIELLFPGWLTGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(14841..15596)
                     /gene="znuC"
                     /locus_tag="ACHOT6_002933"
     CDS             complement(14841..15596)
                     /gene="znuC"
                     /locus_tag="ACHOT6_002933"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFP9948115.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PHGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            15675..16607
                     /gene="znuA"
                     /locus_tag="ACHOT6_002934"
     CDS             15675..16607
                     /gene="znuA"
                     /locus_tag="ACHOT6_002934"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFP9948116.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            16623..17945
                     /gene="mepM"
                     /locus_tag="ACHOT6_002935"
     CDS             16623..17945
                     /gene="mepM"
                     /locus_tag="ACHOT6_002935"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFP9948117.1"
                     /translation="MQQIARSVALAFNNLPRSHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            18065..19036
                     /gene="lpxM"
                     /locus_tag="ACHOT6_002936"
                     /gene_synonym="msbB"
     CDS             18065..19036
                     /gene="lpxM"
                     /locus_tag="ACHOT6_002936"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9948118.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
BASE COUNT         5003 a         4664 c         5030 g         4411 t
ORIGIN      
        1 gtaataaatc atattcttta tcgattattt caggcagtgt tgtgccgaat tatgcaagcg
       61 gttaattcgt tgtatattta attatacaat gatttcggtg ttctggaatt aaattagagg
      121 aatctatgcg agggaaaaaa cgcattgggt tattgttttt gctgatagcg gttgtggttg
      181 gtggcggcgg gttattgctg gcgcaaaaag ccttacataa aacgtcggat acagcatttt
      241 gcctttcctg ccactcgatg agtaaacctt ttgaggaata tcagggaact gtccactttt
      301 cgaaccagaa agggatacgt gcggaatgtg ccgattgcca tattccaaag tcagggatgg
      361 attatttatt tgctaaatta aaagcatcta aagatattta tcatgaattt gttagcggca
      421 aaatagacag tgacgataag ttcgaaactc atcgccagga aatggccgaa acagtatgga
      481 aagaattaaa agccactgac tctgcaacgt gccgtagttg ccattctttt gatgccatgg
      541 atattgcctc gcaaagtgaa tctgcgcaga aaatgcataa caaagcacaa aagggcggcg
      601 aaacctgtat cgattgtcat aaaggcattg cccattttcc gccagaaata aaaatggatg
      661 acaacgcggc gcatgagctg gaaagtcagg ccgctacttc agtgactaat ggcgcacata
      721 tttatccttt caaaacttct cgcataggcg agctggctac cgtgaatcct ggtaccgatc
      781 tcaccgtcgt tgatgccagt ggcaaacagc cgatcgttct gttgcagggt tatcaaatgc
      841 agggcagtga aaacacgctc tacctggcgg caggtcaacg gctggcgcta gccacattaa
      901 gtgaaggagg tatcaaggag ctcacggtaa acggggaatg gcaggctgac gaatacggca
      961 atcaatggcg tcaggcgtct ttacagggtg cgcttaccga tcccgcatta gcggaccgta
     1021 aaccgctatg gcaatacgct gaaaaacttg acgataccta ttgcgctggt tgtcatgccc
     1081 ctattgccgc cgaccattac accgtcaatg cgtggccgtc cattgccaaa ggaatggggg
     1141 cacgaaccag catgagcgaa aacgaactgg acattttaac gcggtatttc cagtacaacg
     1201 ccaaagatat taccgagaaa cagtgatcct cgttgatcag ggaggaagtt atgacattaa
     1261 caagacgtga atttattaaa cacagcggta ttgccgctgg agcgctggtg gtgacatcgg
     1321 cagcaccgct ccccgcatgg gcagaagaga agggcggtaa aatcctcacc gcgggacgtt
     1381 ggggagcgat gaacgtagaa gtgaaggacg gcaagattgt ttcttcaaca ggcgcgctgg
     1441 cgaaaaccat accgaattcc ttacagtcta cggcggcgga tcaggtacac accacggcgc
     1501 gtattcagca tccgatggtg agaaaaagct atctcgataa tccactgcaa ccggcgaaag
     1561 gtcgaggcga agatacctat gtacaggtga gctgggaaca ggcattaaaa ctgattcatg
     1621 agcaacatga gcgtattcgt aaagctaacg gaccatcggc catttttgcc ggatcttacg
     1681 gctggcgctc ttctggcgtg ttgcataaag cgcaaacctt actgcaacgt tatatgaacc
     1741 tggcgggcgg ttattccggg catagcggcg attattcaac cggggcagca caggtgatca
     1801 tgccgcatgt ggtcggttct gtagaggtgt atgaacagca gacctcctgg ccgctgattc
     1861 tggaaaacag ccaggttgtg gtgctgtggg gaatgaaccc gctgaatacc ttaaaaattg
     1921 cctggagcag taccgatgag caagggctgg aatacttcca tcagctgaaa aaatctggca
     1981 aaccagtgat tgccattgat cctatccgct ccgaaaccat cgaattcttt ggcgataatg
     2041 ccacctggat cgcgccgaat atgggcaccg acgtggcact gatgttaggg attgcgcata
     2101 ccctgatgac acaaggcaaa catgataaag tgttccttga aaaatacacc accgggtatc
     2161 cgcagtttga agagtatctg acaggtaaga gcgataatac gccgaagagc gcggcctggg
     2221 cggcggaaat tactggcgta ccggaagcgc agatagtcaa actcgctgaa ctgatggcgg
     2281 cgaatcgcac aatgctgatg gcaggctggg gaattcagcg ccagcaatat ggtgaacaga
     2341 aacactggat gctggtgacg ctggcggcga tgttagggca gattggtaca ccgggcggcg
     2401 gttttggttt ctcttatcat tactccaatg gtggcaaccc gacgcgtgtt ggcggcgtgc
     2461 tgccggaaat gtctgcggcg attgccggac aggccagcga ggccgctgat gatggcggaa
     2521 tgacggcgat tcctgttgcc cgtatcgtcg atgcgctgga aaatcctggt ggcaaatatc
     2581 agcataacgg taaagaacaa acgtatccga atatcaaaat gatctggtgg gcgggtggcg
     2641 gtaactttac ccatcaccag gatactaatc gtctgattaa agcatggcag aaaccggaga
     2701 tgatcgtcgt ttctgaatgc tactggaccg cagctgctaa acatgcagat atcgtattac
     2761 tgatcaccac atcgtttgag cgcaatgact tgacgatgac cggtgattac agcaaccaga
     2821 atattgtgcc gatgaagcag gctgttgctc cgcaatttga agcgcgaaac gattttgacg
     2881 tgtttgccga acttgcggaa ttactgaaac ctggcggaaa agagatctat accgaaggta
     2941 aagatgaaat ggcgtggctg aaatttttct atgatgccgc tcagaaaggt gcccgtgcgc
     3001 aacgcgtgac tatgccgatg tttaatgcct tctggcagca aaataaactg atcgaaatgc
     3061 gccgcagcga gaagaacgaa cagtacgttc gttatgctga tttccgcgcc gatccggtga
     3121 aaaatgcgct gggtacgcca agcggcaaaa ttgagattta ctccaaaacg ctggaaaaat
     3181 ttggctataa ggattgcccg gcacacccaa cctggctggc tcctgatgaa tggaaaggta
     3241 ccgccgacga gaagcagtta cagcttctga ccgcacatcc ggcacaccgt ttacacagtc
     3301 agcttaacta tgcggaactg cgtaaaaaat atgcgattgc cgatcgtgaa ccaatcacta
     3361 ttcacaccga agatgctgct cgctttggta ttgcgaatgg tgatctggtg cgtgtctgga
     3421 acaaacgtgg tcagattctg acaggtgcgg tggtgactga cgggatcaaa aaaggcgtgg
     3481 tatgcgtgca tgaaggtgca tggccagatc tggaaaatgg cttgtgtaaa aacggcagtg
     3541 cgaacgtgtt aacggcggat atccccagct cgcagctggc aaatgcctgt gccggtaact
     3601 ctgcgttggt gtatatcgaa aaatatacgg gcaatgcgcc gaagttaacg gcgtttgatc
     3661 agccagctgt tcaggcgtaa gaagtgaata gcgcccggaa acgggcgctg aggttatgca
     3721 aacgtaaagc tgcctgatgc gctatgctta tcaggcctac atagtccctg caatatattg
     3781 aattagcaag gttttgtagg ccggataagg cgttcacgcc gcatccggca gtaataccag
     3841 acctttacgg cttgcgcgca atcagcaccg cgcgtttagg tgccggataa ccttccaccg
     3901 ttttacccgg atcatgtggg tcgaggaaat cggccagcga ttcggtaacc atccattcgg
     3961 tgcgtcgctg ctcttctgtg gtggtaacgc tcacatccgc aatgcggata tctacaaaac
     4021 cacacttctt cagccagttt tttagcgcca gcgccgaagg gatgaaatag acattacgca
     4081 tttgcgcgta acgatcgccc ggcaccagca ccgtgttctc atcaccatca ataaccagcg
     4141 tttcaagcac cagttcacct tcattgacca gttgatcttt caactgccag agatgctcca
     4201 gcggtgaacg acgatgataa agcacgccca tcgaaaagac ggtatcaaag gctttcagtg
     4261 ccggaagttg ttcaatacct aacggtaaca aatgcgcgcg ctgatcatta cccagtagtt
     4321 tacgcaccgc ttcaaactgg cacagaaaca gttgcgtagg gtcgatcccc accgcgaggt
     4381 gcgcgcctgc gccaatcatg cgccacatgt gataaccgct gccgcagccg acatccagaa
     4441 tggtgcgtcc ggttaaatca gaaagatggg gcagaacacg atcccatttc cagtcggaac
     4501 gccattcggt atcgatgttg acgccataca gtgagaacgg ccctttgcgc catggcatca
     4561 ggttgcgcat aagcgtttca atgcgtttaa tctgcccggc gctcagtggc tcttcgcttt
     4621 cggcggttac gctatgcaat aaatccagac gatacggttt aatttcaggc agaaattcca
     4681 ccgcgttgga ccactgctta aacagcccgt gctgctgctc gcgctgccag ttagcaatct
     4741 gcgcgggcag cgtttcgagc cagtgtgaaa gatgattttt ggcaatcaga gaataaaagt
     4801 taccaaagtc gatcatgcag cgtcctctgc ttttaatgcc accagtgaac caaagttaaa
     4861 gcactggaac cacaactcgc tatgctcaaa accggcgtta tgcaggcgtg ctttatgtgt
     4921 ttccacggaa tcggtcagca tcacgttttc cagcatgctg cgcttctggc tgatctccag
     4981 ttcgctgtaa ccgttggcac gtttaaagtc gtggtgcatg ttgaacagca gttcaccaac
     5041 cttggcatct tcgaaactga atttttccga aagcaccagc gcgccgcccg ggttcagccc
     5101 atgataaatt ttatccagta acgcctggcg ctcggaaggt tccaggaatt gcagggtaaa
     5161 attcagcacc accatcgatg cgttttcaat ggcgatatcg cgaatatcac cttcaataac
     5221 gtctactggc gtcggggctt tataggcgtc aatatgacga cggcagcgtt caatcatcgc
     5281 cggggagttg tcgatggcaa taattttgca attatcatga tgaatgttgc gacgcaccga
     5341 gagcgtcgcc gcgcccagag aacaacccag atcgtaaacc tgcgtacctg gttgaacgaa
     5401 gcgctcggct aacataccaa tcatggaaat aatattggaa tagccgggaa cggaacgctg
     5461 gatcatatcc gggaagactt cagctacccg ttcatcaaag gtccagtcgc ccagtctggc
     5521 gataggggca gaaaatagcg tgtcgcggtg agacataacg taaaaatccg ggaaaaagaa
     5581 agtggcgtat tgtgcgctaa cgcagggaga aaaccaactc ccagggcata taccaaagat
     5641 tcgccagcac catcagcaac agcgcacacc aggtggcgct catgccggaa cgtcgccagc
     5701 gaaacagacg gtgatgaaaa ccgtaataat gcatcagacg accagcaagc aaaacgatgc
     5761 cgcaaatatg caccatccag gtttctgcgc cattcatttc cataaacagc atcagcacaa
     5821 tcgcgatggg aatatattcc accgcgttac catgaatgcg aatagcgctt tgcagttcgc
     5881 taaaaccacc gtcaccatag gcaacgcggt actgcattcg caggcgaacg acatcaaaag
     5941 agaacttcat taataacaac gcacttaaaa cggcatatag cgcgcttacc atacaaactc
     6001 cctttaaaat ggccgatggc acctgtctat gataggtggc aaattcagaa aagagaagat
     6061 tgctgtggaa tagccggaac tgctccgatc tctggaagcg ttttacgtaa gtcttcccac
     6121 agggtatgta ccagttccgg ggcctgggca atgtctggcg tatgtaaaaa aagataaggc
     6181 gtagtggtct gatgccactg cgctaatttc tgtaaccaga cctgaaataa ttcccggttt
     6241 tgcgtcatat catcactacc gataaaacga atcagcggat ttgtcgccgt cagtacagca
     6301 tgtaccggaa ctttaggttt ttttcgttga gcgtcgcgaa tagcttcact gtgtggacgt
     6361 gctgcatgaa cagggcggct gtctaaaatc acccgattaa cgccgcgctg atgtaaaccg
     6421 cgattaagcg tttgttcctc ttcccctttg gcgaaaaact gtggatggcg gacttccaca
     6481 ccataattaa attcgccagg gagagaatcg agaaaatgcc aaagcgcagg cagctcccgt
     6541 gggccgaatg tggcaggcag ttgcagccag tattgcccaa tgcgcggagc caacggtgac
     6601 atgcgggtca aaaattcagt cactaaatca tcgcaatgtc gtaatgctgc ctgatgcgaa
     6661 atggtcgccg gaaacttaaa acagaagcgg aagtcatctg tggtctgttc acgccagcgc
     6721 aggacaacct cgggtttcgg cagggcgtaa agcgtggtgt tgccctccac gcagttaaag
     6781 tggcgggcat actcttcaag gctggtgatc cccaaccgca cccatttagg atgcgaccat
     6841 tgcggtagac cgatgtaaat cataacgcgt gaaggatctc ttccacgcta cgcacacggg
     6901 cgatgcgcgg gtagatatga ttaatgctgt tattgtgttg ctcggcgctg gcggcactac
     6961 aggcgtcttc ggcaatcacc agattaaagc caagttccca ggcattgcgg gcggtggatt
     7021 caacaccgat attggtcgag atcccacata acactattgt atcgatacct cggcggcgca
     7081 gttgcaactc cagatccgta ccgtaaaacg caccccactg acgtttgatg atttcgatat
     7141 cactgtcagt tgcacctaat gcagctggat gttgccacca gttttcgggc aacactttag
     7201 cggggaaggg ggcatcaacc ggctgtttta atgcttcggc gtgatcggca gaccagccaa
     7261 cgcgcaccag aaacacgggc tgaccgctgg cgcgaaattt cgccgccagc ttcccggcgc
     7321 gattaactac ctcatcggca gtatgtggac caccggcaaa aggtaagatg ccttcttgta
     7381 aatcgatcac caccaacgca gtggttttag cattaagttc aagcatgctg actcctgaaa
     7441 aaacaaaatc gtgcctcaca ccttaatgcg attagccgat ggcggaacga caaattttgt
     7501 taatttttat gagttcgggc aacaccttta tagcaaactg gcgtagagtc tgatttcagg
     7561 cccgatataa agtggtaacg aggcgttcca gcgcgggcca ggctcttacg gtgcggcaga
     7621 atttccagta taatagccgc cttttttcat tcagttgtga catacagcta acgctgcgac
     7681 ttggtcacct gcgcagcagg gggcatccgc cggcagctaa gttaagggat atctcatgcg
     7741 tacagaatat tgtggacagc tccgtttgtc ccacgtgggg cagcaggtga ctctgtgtgg
     7801 ttgggtcaac cgtcgtcgtg atcttggtag cctgatcttc atcgatatgc gcgaccgcga
     7861 aggtatcgtg caggtatttt tcgatccgga tcgtgcggac gcgttaaagc tggcctctga
     7921 actgcgtaat gagttctgca ttcaggtcac gggcaccgta cgtgcgcgtg acgaaaaaaa
     7981 tattaaccgc gatatggcga ccggcgaaat cgaagtgctg gcgtcctcgc tgactatcat
     8041 caaccgcgca gatgttctgc cgcttgactc taaccacgtc aacaccgaag aagcgcgtct
     8101 gaaataccgc tacctcgacc tgcgtcgccc ggaaatggct cagcgcctga aaacccgcgc
     8161 taaaatcacc agcctggtgc gccgttttat ggatgaccac ggcttcctcg acatcgaaac
     8221 tccgatgctg accaaagcca cgccggaagg cgcgcgtgac tacctggtgc cttctcgtgt
     8281 gcacaaaggt aaattctacg cactgccgca atccccacag ttgttcaaac agctgctgat
     8341 gatgtccggc tttgaccgct actatcagat cgttaaatgc ttccgtgacg aagacctgcg
     8401 cgctgatcgt cagcctgaat ttactcagat cgatgtggaa acttctttca tgaccgcccc
     8461 gcaagtgcgt gaagtgatgg aagcgctggt gcgccatctg tggctggaag tgaagggcgt
     8521 ggatctgggc gatttcccgg taatgacctt tgcggaagca gaacgccgtt atggttctga
     8581 taaaccggat ctgcgtaacc cgatggaact gaccgacgtt gctgatctgc tgaaatctgt
     8641 tgagtttgca gtatttgcag gtccggcgaa cgatccgaaa ggtcgcgtag cggctctgcg
     8701 cgttccgggt ggcgcatcgc tgacccgtaa gcagatcgac gaatacggta acttcgttaa
     8761 aatctacggc gcgaaaggtc tggcttacat taaagttaac gaacgcgcga aaggtctgga
     8821 aggtatcaac agcccggtag cgaagttcct taatgcagaa atcatcgaag ccatcctgga
     8881 tcgtactgcc gcgcaagatg gcgatatgat tttcttcggt gccgacaaca agaaaattgt
     8941 tgccgacgcg atgggcgcac tgcgcctgaa agtgggtaaa gaccttggtc tgaccgacga
     9001 aagcaaatgg gcaccgctgt gggttatcga cttcccgatg tttgaagacg acggagaagg
     9061 cggcctgacg gcaatgcacc atccgttcac ctcaccgaaa gacatgacgg ctgcagagct
     9121 gaaagctgca ccggaaaatg cggtggcgaa cgcttacgat atggtcatca atggttacga
     9181 agtgggcggt ggttcagtac gtatccataa tggtgatatg cagcagacgg tgtttggtat
     9241 tctgggtatc aacgaagagg aacagcgcga gaaattcggc ttcctgctcg acgccctgaa
     9301 atatggtact ccgccgcacg caggtctggc attcggtctt gaccgtctga ccatgctgct
     9361 gaccggcacc gacaatatcc gtgacgttat cgccttcccg aaaaccacgg cggcagcgtg
     9421 tctgatgact gaagcaccga gctttgctaa cccgactgca ctggctgagc tgagcattca
     9481 ggttgtgaag aaggctgaga ataactgata tgactcaaat acacgaaatc attcgcgttg
     9541 catcgaggcg gcaactgagt gaactcccat gagcatagat aactatgtga atgggatgag
     9601 cgaaggcagt caacgaagag gcagcgtgaa ggataaagtg tataagcgtc ccgtttcgat
     9661 cttagtggtc atctatgcac aagatacgaa acgggtgctg atgttgcagc ggcgtgacga
     9721 tcccgatttc tggcagtcgg taaccggcag cgtggaagag ggtgaaaccg cgccgcaagc
     9781 tgccatgcgc gaagtaaagg aagaggtcac cattgatgtt gtcgctgaac aactgacctt
     9841 aattgactgt cagcgcacgg tagagtttga aattttttca catttacgtc atcgctatgc
     9901 gccgggcgtg acgcgtaata cggaatcatg gttctgtctt gcgcttccgc acgagcggca
     9961 gatcgttttc actgaacatc tggcttacaa gtggcttgat gcgcctgctg cggcggcgct
    10021 cactaagtcc tggagcaacc ggcaggcgat tgaacagttt gtaattaacg ctgcctgaac
    10081 aggcagggct atctggagat atttttatgg caggtcatag taaatgggcc aacaccagac
    10141 atcgtaaagc tgcgcaggat gctaagcgcg gtaaaatctt cactaaaatc attcgtgagc
    10201 tggtaaccgc ggctaagctg ggcggtggcg atccggacgc taacccgcgt ctgcgtgcgg
    10261 cggtggataa agcactgtct aacaacatga cccgtgacac actgaaccgc gcaattgcac
    10321 gtggtgtggg cggtgatgat gatgcaaaca tggaaaccat catctacgaa ggttacggtc
    10381 ctggcggcac ggcaatcatg attgaatgtc tgtctgacaa ccgcaaccgt accgttgctg
    10441 aagtgcgtca cgcattcagc aaatgtggcg gtaacctcgg cactgacggt tccgtagcct
    10501 atctgttcag caaaaaaggc gtgatctcct tcgagaaagg cgacgaagac accatcatgg
    10561 aagcagctct ggaagcaggt gctgaagacg ttgtgacata tgatgacggc gcgattgatg
    10621 tctacactgc atgggaagaa atgggtaaag tgcgcgacgc tctggaagcg gcaggtctga
    10681 aagcagacag cgcggaagtt tccatgatcc cgtccaccaa agctgatatg gatgcagaaa
    10741 ccgcaccgaa actgatgcgt ctgatcgata tgctggaaga ttgcgacgac gtgcaggaag
    10801 tttaccataa cggtgaaatc tctgatgagg tcgcagcgac tctctgatga ggcctgctaa
    10861 acagcaaaac ggagacgcgt gatggctatt attctcggca ttgacccggg ttcgcgcgtg
    10921 accggctacg gcgtcatccg ccaggtaggt aggcaactgt cctacctggg tagcggatgc
    10981 atacgcacca aagtggatga tttaccgtct cgtctgaagc tcatctatgc gggcgtgacg
    11041 gaaatcatca cccagttcca gcctgattat ttcgccattg aacaagtctt tatggccaaa
    11101 aatgccgact cggctctgaa actggggcag gcgcgcggcg tggcgattgt ggcggcggtg
    11161 aatcaggaat tgccggtatt tgaatatgcg gcacgtcagg taaagcaaac ggtggtaggt
    11221 attggtagtg ccgaaaaaag tcaggtgcag catatggtcc gcaccttgct gaaactgccc
    11281 gctaatccac aggcggatgc cgccgatgcg ttagctatcg cgattaccca ttgtcacgtc
    11341 agccagaatg caatgcagat gagcgaatcg cggctgaacc tggcgagagg gcgactgcgt
    11401 taagttatac cgcctcggtc agttcaggct gaggcgtttc aactccctcc gcgtgaatca
    11461 acaccaaacc cggatgatgc cacaaactgg ctggcgtgac ggttttacgc tcccacggaa
    11521 tagaacccag gaaccagaat ttccagaaag tgagttttgc attcggattg ctatttaaca
    11581 attctccaaa cgtctctatt tcacccaccg gaatacatag cgcttcttta taaatatcaa
    11641 aaacaaattt tgaacagaac tggcgcgaag attcgtattt aaaacccgtg tggtaaagtt
    11701 tgcgtagccg ggagggaacc tgttcaacaa ttcgttgttt ttgttgttct gtcagcccag
    11761 cgtctaatcg ctttatagca tagcgttgat tagacgaacg tttaataaaa cgagatagcg
    11821 tggtgatggt tgaaagggga acccggcttt ctgcaaccag gaagtcttca ccgttatgac
    11881 cgataatgat cccgacgtga ttactccagc aatttgatgc agctgatatt tgaccaaata
    11941 aggcagcacc tatacatgta aagacgatat ctccaatttc atattcagca gggtaattaa
    12001 tattcacgtt gtcattccat tgaaatagat acgaatatac gtaatagcat aaaaaattta
    12061 ttttttcagg agtcgcaatg tatattttta ttacgcattt cttcactgaa tatgtaatat
    12121 tcaaatattt gcttccaata taacctgttg aataaattat actgtgccat ttttcagatc
    12181 atcgggacac ctcgcaagtt ttcttcatcc ttcgctggat atctatccag cattttttta
    12241 tcatacagca ttatctttga ttcattacgc aggagcgtca tgtgataggc agactcagag
    12301 gcatcatcat tgaaaaacaa cccccgctgg tgttaattga agtgggcggc gtaggctatg
    12361 aagtgcatat gccgatgacc tgtttttatg aactccctga agcgggtcag gaagcgatcg
    12421 ttttcaccca ctttgtggtg cgtgaagacg cgcaactgct gtacggtttt aacaataaac
    12481 aagaacgcac gttgttcaaa gagttgatca aaaccaacgg cgtcggcccg aagttggccc
    12541 tggcgatcct ctccggaatg tcagcgcagc agttcgttaa tgccgttgag cgtgaagaag
    12601 tgggggcact ggtgaaactg ccgggtattg gcaaaaaaac cgccgaacgc ttgattgttg
    12661 aaatgaaaga ccgatttaaa ggtttgcatg gcgatctctt tacgccagcc gccgacctgg
    12721 tactcacgtc tcctgtcagc ccggcgaccg acgatgctga acaagaagca gttgccgcgc
    12781 tggtggcgct aggctataaa ccacaagaag caagccgcat ggtgagcaaa atcgctcgcc
    12841 ctgacgccag cagtgaaaca ttaattcgcg aagccctacg cgccgcgtta tgaggtaaag
    12901 gatgattgaa gcagaccgtc tgatttctgc cggtaccact ttgccggaag atgtggcaga
    12961 tcgcgccatt cgccctaaat tactggaaga gtatgttggt cagccgcagg ttcgttcaca
    13021 gatggagatt ttcatcaaag cagcgaaact gcgcggcgat gccctcgatc atctgttgat
    13081 ttttggtcct ccggggttgg gtaaaactac gcttgccaac attgtcgcca atgaaatggg
    13141 cgttaatttg cgcacgactt ctggtccggt gctggaaaag gcgggcgatc tggcagcgat
    13201 gctcactaac cttgaaccgc atgacgtgct gtttattgat gagatccacc gtctttcacc
    13261 agtggtggaa gaggtgttgt atccggcaat ggaagattac caactggata tcatgattgg
    13321 tgaaggtccg gcggcacgct ccattaaaat tgatttgccg ccgtttaccc tgattggtgc
    13381 aaccacgcgc gcaggttcgc tgacatcacc gttgcgcgac cgttttggta ttgtgcaacg
    13441 tctggagttt tatcaggtgc cggatctgca atatatcgtc agtcgcagcg cacgctttat
    13501 gggacttgag atgagtgatg acggcgcgct ggaagttgct cgtcgcgctc gcggtacgcc
    13561 acgcattgcc aaccgtctgc tgcgtcgagt gcgtgatttc gccgaagtga agcacgatgg
    13621 caccatctcg gcagatatcg ctgctcaggc gctggatatg ttgaatgtcg atgctgaagg
    13681 tttcgattat atggaccgca aattgttgct ggcggtaatc gataagttct ttggtgggcc
    13741 ggtaggtctg gataaccttg cggcagccat tggcgaagaa cgtgaaacca ttgaggatgt
    13801 gctggaacct tatttgattc agcaaggctt tttgcagcgt acaccgcgtg ggcgtatggc
    13861 gacgacgcgg gcgtggaatc actttggcat aacgccgcca gaaatgccgt aagtcggatt
    13921 ggtcgctcgc gccgcatccg acactggcag tgtgtctgat gcgacactgt tcgcgtctta
    13981 tcaggccagc cagagatagc gaaccgtagg tcggataagg cgctcgcgcc gcatccgaca
    14041 aatgtgttca gcgatagatt agctggcctg ctttttcatc atactgagaa taaataacag
    14101 tgccgcacat agcaccaccg acggacccgc cggcgtatcg taaaacgcgg aaaaggttaa
    14161 accgccagtt actgccacca ttcccaccaa aacagcgaca ccagccatct gttccggcgt
    14221 acgggcaaag cgacgcgcag tagctgcagg aataatcagc agtgaagtaa taatcaacgc
    14281 gccaacaaat ttcatcgcta caccaatcgt caatgccgtc accagcatca acaacaattt
    14341 cacgcgctgt aatttcacac catcaacaaa cgccagatcc gggctaatcg tcatcgacag
    14401 caaattgcgc cattgccaga acaaaatagc caccacgatg accacgccaa tcgcaatagc
    14461 gatgagatct tctggcgtca ctgccagcaa atcaccgaac aggtaagcca tcaaatcaac
    14521 acgaatgtta gacatcagac taacgaccac caggcccagc gacagggcac tgtgcgccat
    14581 aatccctaat aacgtgtcaa tcgccagctg tggacgcttc tccagccata ccagaccgcc
    14641 cgccaggagc agcgtaacgg caattaccgc atagaatgga ttcacgtcca gcaacaaacc
    14701 aaacgcgaca ccaagtaatg aggcatgagc cagcgtatca ccgaaataag acatacgacg
    14761 ccagactaca aacgaaccca gcggacccgc ggcacaggcg agcatgatcc cggttaacca
    14821 accgggaaat aataattcaa tcatgagcga tcatttcccc gacgcaaaac aattcgtccc
    14881 tgtaaatcgt gacgatgatt atgatgatgg cgatagatac ccagttgttc agcaccatga
    14941 ggaccaaaca tagaaataaa ctccggatgt agggaaacaa cttccggtgt gccggaacaa
    15001 caaatgtggt gattcagaca cagcacttca tcggtttttg ccattaccag atgcagatcg
    15061 tgagagacca ttaaaacgcc acaatccagt tcgcgacgca actggtcaat aaggtcatat
    15121 aacgccacct ggccatttac atccacgcct tgagtgggtt catccagcac taataattgc
    15181 ggtcgattta acaatgctcg cgctaacagt acacgctgtg tttcgccacc agagagcttt
    15241 tgcatcggtg cgttaatcag atgcccggcc tggacacgtt tcagtgcagg caaaatatct
    15301 tctttatgtg tgccagggcg taagcgtaaa aaacggttta cggtcagtgg caacgtggtg
    15361 tcgagataca gcttctgcgg tacatagccg atgcgtagtt ttccgttgcg cttgataacc
    15421 ccttcatcgg gtgttaccag cccgagcact acccgtacca gtgtcgactt acctgcgcca
    15481 tttggcccaa gtaaagtcaa aatttttcca ggtttaagtt ccagcgacac atcagagagg
    15541 acgcggcgtt ggccaaaaga aaccgagaca ttttccaggg aaaccagact tgtcatgtta
    15601 attttagtct tgcagtagtc atgaaatgtt ataatatcac acttctcata ttcattacga
    15661 tgattggtcg cattatgtta cataaaaaaa cgcttctttt cgcagcatta tccgccgctc
    15721 tctggggagg tgcaacacag gccgcagatg ccgccgttgt cgcttcgctt aaacccgttg
    15781 ggttcatcgc ttctgccatt gctgatggtg taacagaaac ggaggtttta ctgcctgacg
    15841 gcgcttcaga acatgattat tcactgcgtc catcggatgt aaaacgctta cagaacgcgg
    15901 acttagtcgt ttgggttggc ccggagatgg aagcgtttat gcaaaaaccg gtaagcaaat
    15961 taccaggagc gaagcaggta acgattgcgc agcttgaaga tgtgaaaccg ctactgatga
    16021 aaagtattca cggcgatgat gatgatcacg accacgcgga aaaaagtgac gaagatcacc
    16081 atcacggcga tttcaacatg catctttggc tttccccaga gatagcgcgg gctacagcgg
    16141 ttgcaatcca tggaaaatta gtggaactta tgccgcaaag tcgagccaaa cttgacgcca
    16201 acctgaagga ttttgaggca caattagcct caaccgaaac gcaggttggt aacgagctcg
    16261 cgccgctcaa ggggaaaggt tatttcgttt ttcacgatgc ttacggctat ttcgaaaaac
    16321 agttcggact gacaccgctt ggtcatttta ccgttaaccc tgagattcaa cctggcgcgc
    16381 agcgtttaca tgaaataaga acacagttgg ttgagcaaaa agcaacctgc gtttttgctg
    16441 agccacagtt caggccagcg gtcgttgaga gcgtcgcacg agggacatcc gttcgtatgg
    16501 gaacgttgga tcccctgggg acgaatatca aactgggtaa aacaagctat tcagaattcc
    16561 tgagtcaatt agccaaccag tatgcgagct gcctgaaagg agattaatga ggaagtgatt
    16621 acgtgcaaca gatagcccgc tctgtcgccc tggcgtttaa taatttaccg cgatcacacc
    16681 gcgttatgtt ggggtcgctc accgttctta ctctggccgt cgctgtctgg cggccttatg
    16741 tttatcaccg tgatgccacg ccaattgtca aaaccattga gctggaacag aacgaaattc
    16801 gttcgctctt acctgaagcc agtgagccga ttgatcaagc tgcacaagaa gatgaagcca
    16861 ttccccagga cgaactggat gacaaaatcg ccggtgaagc gggcgtgcat gaatatgttg
    16921 tttccactgg cgatacgcta agcagcattc tcaatcagta tggtattgat atgggtgata
    16981 tcacccaact ggctgcggcc gacaaagaat tgcgtaacct gaaaatcggt caacaactct
    17041 cctggacatt aaccgcggac ggcgaactgc agcgcctcac ctgggaagtg tctcgtcgtg
    17101 aaacccgaac ctatgaccgt actgccgcta acggttttaa aatgaccagc gaaatgcagc
    17161 aaggagagtg ggttaacaat ctgctgaaag gtaccgtcgg ggggagcttt gttgccagcg
    17221 ccagaaacgc cggtttaacc agcgccgaag tgagcgcagt gattaaagcc atgcagtggc
    17281 aaatggattt ccgcaaactg aaaaaaggcg atgaatttgc ggtattaatg tctcgagaaa
    17341 tgcttgatgg taaacgtgag caaagccagc tgctgggcgt acgtttgcgt tcagaaggta
    17401 aagattatta cgctatccgc gctgaagatg gcaaattcta cgatcgtaac ggtactggtc
    17461 tggcgaaagg attcttgcga ttcccgacgg cgaaacagtt ccgtatctcg tctaacttta
    17521 acccgcgtcg tactaatccg gtgaccggtc gcgttgcacc acacagaggt gttgatttcg
    17581 ccatgccgca gggtacgcca gtgctttcag tgggtgacgg tgaagtggtg gttgccaaac
    17641 gcagtggcgc agcaggttat tatgtggcta ttcgtcatgg tcgcagctac accacgcgtt
    17701 atatgcactt gcgcaagatt ctggtgaaac cgggacagaa ggtgaaacgt ggcgaccgta
    17761 tcgcgctttc cggtaatacc ggacgttcaa ccgggccgca tctgcactat gaagtatgga
    17821 taaaccagca ggccgtaaac ccgctgacgg caaaactgcc gcgtaccgaa gggctgaccg
    17881 gctccgatcg tcgcgaattc ctggcgcagg ccaaagagat tgtgccgcag ctacggtttg
    17941 attaattaac atccattcgc agccggtacg cagtcagtac cggctttttt tatttggtgc
    18001 ggggcaagtt gcgccgctac actatcacca gattgatttt tgccttatcc gaaactggaa
    18061 aagcatggaa acgaaaaaaa ataatagcga atacattcct gagtttgata aatcctttcg
    18121 ccacccgcgc tactggggag catggctggg cgtagcagcg atggcgggta tcgctttaac
    18181 gccgccaaag ttccgtgatc ccattctggc acggctggga cgttttgccg gacgactggg
    18241 aaaaagctca cgccgtcgtg cgttaatcaa tttgtcgctc tgctttccag aacgtagtga
    18301 agctgaacgc gaagcgattg ttgatgagat gtttgccacc gcgccgcaag cgatggcaat
    18361 gatggctgag ttggcaatac gcggaccgga gaaaattcag ccgcgcgttg actggcaagg
    18421 gctggagatc atcgaagaga tgaggcgtaa taacgagaaa gttatctttc tggtgccgca
    18481 cggttgggcc gtcgatattc ctgccatgct gatggcctcg caagggcaga aaatggcagc
    18541 gatgttccat aatcagggca acccggtttt tgattatgtc tggaacacgg tgcgtcgtcg
    18601 ctttggcggt cgtctgcatg cgagaaatga cggtattaaa ccattcatcc agtcggtacg
    18661 tcaggggtac tggggatatt atttacccga tcaggatcat ggcccagagc acagcgaatt
    18721 tgtggatttc tttgccacct ataaagcgac gttgcccgcg attggtcgtt tgatgaaagt
    18781 gtgccgtgcg cgcgttgtac cgctgtttcc gatttatgat ggcaagacgc atcgtctgac
    18841 gattcaggtg cgcccaccga tggatgatct gttagaggcg gatgatcata cgattgcgcg
    18901 gcggatgaat gaagaagtcg agatttttgt tggtccgcga ccagaacaat acacctggat
    18961 actaaaattg ctgaaaactc gcaaaccggg cgaaatccag ccgtataagc gcaaagatct
    19021 ttatcccatc aaataaaaaa cagcctctca aattattgag aggctgtact tgataaaaaa
    19081 tctcaatgag tgttctcacc attttgag
//
LOCUS       DAZKIV010000086        19038 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZKIV010000086 DAZKIV010000000
VERSION     DAZKIV010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19038
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.086"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHOT6_002937"
     CDS             complement(134..2401)
                     /gene="adiA"
                     /locus_tag="ACHOT6_002937"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFP9948119.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHOT6_002938"
     CDS             complement(2600..3508)
                     /gene="melR"
                     /locus_tag="ACHOT6_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFP9948120.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3791..5146
                     /gene="melA"
                     /locus_tag="ACHOT6_002939"
     CDS             3791..5146
                     /gene="melA"
                     /locus_tag="ACHOT6_002939"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFP9948121.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAVASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5249..6670
                     /gene="melB"
                     /locus_tag="ACHOT6_002940"
     CDS             5249..6670
                     /gene="melB"
                     /locus_tag="ACHOT6_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFP9948122.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHOT6_002941"
     CDS             complement(6809..7438)
                     /gene="yjdF"
                     /locus_tag="ACHOT6_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFP9948123.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7561..9206)
                     /gene="fumB"
                     /locus_tag="ACHOT6_002942"
                     /pseudo
     CDS             complement(7561..9206)
                     /gene="fumB"
                     /locus_tag="ACHOT6_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
     gene            complement(9284..10624)
                     /gene="dcuB"
                     /locus_tag="ACHOT6_002943"
     CDS             complement(9284..10624)
                     /gene="dcuB"
                     /locus_tag="ACHOT6_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFP9948124.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTPHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11195..11914)
                     /gene="dcuR"
                     /locus_tag="ACHOT6_002944"
     CDS             complement(11195..11914)
                     /gene="dcuR"
                     /locus_tag="ACHOT6_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFP9948125.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11911..13542)
                     /gene="dcuS"
                     /locus_tag="ACHOT6_002945"
     CDS             complement(11911..13542)
                     /gene="dcuS"
                     /locus_tag="ACHOT6_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFP9948126.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13723..13953
                     /gene="yjdI"
                     /locus_tag="ACHOT6_002946"
     CDS             13723..13953
                     /gene="yjdI"
                     /locus_tag="ACHOT6_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFP9948127.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13965..14237
                     /gene="yjdJ"
                     /locus_tag="ACHOT6_002947"
     CDS             13965..14237
                     /gene="yjdJ"
                     /locus_tag="ACHOT6_002947"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9948128.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            complement(14470..15167)
                     /locus_tag="ACHOT6_002948"
     CDS             complement(join(14470..14918,14918..15167))
                     /locus_tag="ACHOT6_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558260.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1-like element IS1B family transposase"
                     /protein_id="HFP9948129.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMAMNGVGCRATARIMGVGLNTIFRHFKKLRPQSVTSRIQPGSDV
                     IVCAEMDEQWGYVGAKSRQRWLFYAYDRLRKTVVAHVFGERTMATLGLLMSLLSPFDV
                     VIWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNLRQHLARLGRKSLSFSKSVELH
                     DKVIGHYLNIKHYQ"
     gene            complement(15221..15700)
                     /locus_tag="ACHOT6_002949"
                     /pseudo
     CDS             complement(15221..15700)
                     /locus_tag="ACHOT6_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
     gene            16019..17296
                     /gene="yqcE"
                     /locus_tag="ACHOT6_002950"
     CDS             16019..17296
                     /gene="yqcE"
                     /locus_tag="ACHOT6_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFP9948130.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLTLTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            17323..18801
                     /gene="ygcE"
                     /locus_tag="ACHOT6_002951"
     CDS             17323..18801
                     /gene="ygcE"
                     /locus_tag="ACHOT6_002951"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFP9948131.1"
                     /translation="MSKKYIIGINGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         4630 a         4560 c         4895 g         4953 t
ORIGIN      
        1 cggggtgggg ccgcgcaaaa agtatgacat ttttgtgtgc agcaattttt atgaaaacgt
       61 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      121 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
      181 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
      241 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      301 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
      361 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
      421 ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      481 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      541 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      601 aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca
      661 ggaacataat ttggaagtca gtggtgcgag taggcacaat gccgtggcga ccaagccagg
      721 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      781 gagcaaggat gctgacttta attgggtcga gcatactcca gttatccgga atatctttga
      841 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      901 ttgttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
      961 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatac agccgcgcca
     1021 tcgcctgacg aaaatcaacc gcttcgtcga tcacttcctg cgtcagtgac aggccgctgt
     1081 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1141 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1201 cacgtacatg aatataagaa gcctgtgaaa gcgcattcag cagtttgtgg gtggagtggg
     1261 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1321 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1381 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1441 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt
     1501 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1561 tgccgtagcg gttgcggctt ggcaccatgt agactggttt cgcgcctgtc agcatcaagc
     1621 cttgttcgat ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc
     1681 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1741 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1801 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat
     1861 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1921 ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg
     1981 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
     2041 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcagg tcgcgatcca
     2101 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2161 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2221 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2281 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2341 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2401 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2461 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttatttttat aaacaatgtg
     2521 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2581 gctgatactg atatcgtctt tagccgggaa acgtctgacg gcgctgttgg ctaagtttgc
     2641 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2701 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2761 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc
     2821 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2881 tggtcagcgc ctggtcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     2941 gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt
     3001 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3061 gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3121 gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3181 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac
     3241 aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct
     3301 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3361 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3421 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3481 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3541 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3601 aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt
     3661 accataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3721 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3781 ggagatctgc atgatgtctg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3841 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3901 cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat
     3961 ggattcagca gtggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca
     4021 ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac
     4081 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4141 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4201 gacggaagtc tgccccgatg ccaccatgct caactacgtt aacccaatgg cgatgaatac
     4261 ctgggcgatg tatgcccgct atccgcatat caaacaggta gggctgtgcc attcggtgca
     4321 gggaacggcg gaagagctgg cgcgtgacct caatatcgat ccgtccacgc tgcgttaccg
     4381 ttgcgctggt atcaaccata tggcgtttta cctggaactg gagcgtaaaa ccgccgacgg
     4441 cagttacgtg aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa
     4501 gccaaatatt catggtaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa
     4561 gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat
     4621 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4681 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4741 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4801 tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4861 acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa
     4921 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     4981 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat
     5041 ggacccgcat actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct
     5101 gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5161 ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg
     5221 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5281 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5341 ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5401 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5461 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5521 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt
     5581 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5641 tccaaccatc acgctcgata aacgtgagcg tgaacaactg gttccttatc cgcgtttttt
     5701 tgccagtctg gcgggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5761 cggtggcgat cggggatttg gctttcagat gttcactctg gtactgatcg ccttttttat
     5821 tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5881 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgcttattt ataaaaacga
     5941 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca atattattac
     6001 cggctttgct atctattatt tctcatatgt tattggtgat gcggatttgt tcccctatta
     6061 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6121 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6181 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6241 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga
     6301 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt
     6361 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6421 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6481 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
     6541 tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc tggataaata
     6601 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6661 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgtgttatgt ttatcaggcc
     6721 acgtcttaat acaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6781 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
     6841 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
     6901 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     6961 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7021 ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga
     7081 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7141 acggattacg actcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt
     7201 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7261 gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca ataatcaccg
     7321 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
     7381 ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     7441 tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7501 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7561 ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt cgttaccttt
     7621 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7681 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     7741 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7801 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     7861 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataccg
     7921 gcaggggttt tcgccggacc cgcgtagtag atcgggtgat ctttgatgta ctgcggaagt
     7981 tctttaccgg cgtcaatcag ctctttcagc ttggcgtgtg caatatctcg gcccacgata
     8041 atggtgccgg tgagcgacaa acgagtggaa accggatatt gcgaaagctg ggcgaggatc
     8101 tctttcatcg ggcggttaag gtcaactttc accgcttcgc cttcaccggc ctggcgcagt
     8161 tcttccggaa tgtactggcc tgggttgtgt tccagttttt cgatccagat accttcgcgg
     8221 ttgattttcg ctttaatgtt acggtcagcg gagcaggaga cgcccatgcc gaccgggcag
     8281 gatgcgccgt gacgtggcag acggataacg cgaatgtcgt gcgcgaagta tttaccgcca
     8341 aactgcgcgc caagaccgag tttctgggcc tcttccagca gttcctgttc cagctggaca
     8401 tcgcggaacg cctgaccatg ttcgttccct tccgtcggca gttcatcgta atagtgagcg
     8461 cttgctaact tgacggtttt caggttggtt tccgcagacg taccgccaat cacaaacgcg
     8521 atatggtacg gcgggcaggc tgcagtaccg agggtacgca ttttctcgac gaggaagttt
     8581 ttcagtttgc cgggagtcag cagggctttg gtttcctggt agagatacgt tttgttggca
     8641 gagccgccgc ctttcgcaac gcaaaggaat ttgtactcat cgccatctac cgcgtacagg
     8701 tcgatttgcg caggcaggtt agtgccggtg ttgacctctt tgtacatgtc cagcgccgcg
     8761 ttttgcgagt agcgcaggtt atcttcgata taggtgttat agacgccttt cgacagcgct
     8821 tcttcatcac cgccaccggt ccacacgcgc tggccttttt taccgacgat gatcgcggtg
     8881 ccggtatcct ggcaggtcgg cagcacgcct ttggcggcga tttcggagtt tcttaagaat
     8941 tgcagcgcca cgtacttgtc gttttcgctg gcttctggat cgtgaagaat agccgcaacc
     9001 tgtttctggt gtgccggacg gagcatgaaa gaagcgtcgt gaaaggcttg ctgcgccagt
     9061 agggtcaggg cttctggttc cactttcagg atggtttcgc cgtcgaagtc ggcaacgcta
     9121 acgtaatcgg aagtgagtag atagtattcg gtattgtctt tccccatcgg gaaaggtgcc
     9181 tggtagataa agggtttgtt tgacatagct tccagcctgt aactctgtat ttgttattcg
     9241 aaaatggcgt gccgcgtagt cacgggcggc acgcaaagtg catttataag aacccgtaca
     9301 tcgcggcgaa gatccagccg aagacgcacg atacgctcac accaatcaac cccggcagaa
     9361 taaaactgtg gttgatgacg aagcgaccga tgtggggggt gccggaacgg tcaaactgaa
     9421 tcgctgccag atcgctcgga taagtcggca ggatgtaata accgtagcaa gccggtgctg
     9481 aagccacgat gtatgccgga tcaacgccga tcgccagcgc gaccggaaca atcgccgcca
     9541 gcgccgcagc ctgagagttt acaaacttgg aaaccagcag cagaacaatg gcatacgccc
     9601 acggatactc tttcaccatt tcacccagta cgccctgaat ttcagacata tgcgcaccga
     9661 acatggtttc tgccatccat gcgataccgt acaccgccac gatggcgatc ataccggaac
     9721 ggaagacttc gttttttgag atagacgcgg gattggtttt ggtcaggata ataatcagcg
     9781 ccccggtcag cagcataaac atctgaataa ccagtaccat cgacagcggt ttgccgccga
     9841 aggatggacg caggtccgaa tcagcaccaa gaagggcgac tacagcgatt gccccgagga
     9901 aaatccacat tgccagccag ttgcttttcg gcagtttttt atccagcagc gtcgcggtat
     9961 caccgtaaac atactcacgg ttttccggta cggagatgaa tttctggaac tcttcgtctt
    10021 tatccagatc tttaccgcgg aaccagctga agataccgat cgccaggata ccgattaacg
    10081 tcgatggaat ggtgattgcc agcagatcga ggaactcaag atggcgacca tcaaaggtga
    10141 cattacccag catcgcaacc agagacacga ccgcaaccga caccggactg gcgataatcc
    10201 ccatctgtgc accgatagaa cttgccgcca tcggacgttc cggacggatg ttgttcttaa
    10261 tggcgacgtc gtagatgatc ggcagaatgg tgtaaaccac atgacccgta ccgcaaagaa
    10321 tggtcagtgt acaggtcaca aacggcgcga caattgagac atatttcggg ttgcggcgca
    10381 gcagcttctc ggcaatttgc agcatgacat caagaccgcc cgaagcttgc aaggtcgccg
    10441 atgccgccac caccgcaatg ataaccagca tgacatcaac tggtggttta cctggctgaa
    10501 ggtggaagac gaagaccaga atgaccagac cgataccgcc taataaaccc agcgcgatac
    10561 caccctttct ggcaccataa aacagacata tcagtattat gataagttgg atagtaaata
    10621 acatgtgtga accctcgcga taatcctatt taaatttttg ctgaatagat cacagtcacg
    10681 ttctgttttg tatgaactgt tttcagagcc tgactaaata tccgtttggt cgttaccggc
    10741 tttagcaaat gcctcacagt gaatattggc tgattaaagc agcggcaaat ttatgcactg
    10801 tgataagcgg ctttttcaga gagaaaaact ctctggcgat gggtttatag tttgtactgc
    10861 gcagatgtta atgttttgtt tgctgttatc ttattgatat ttatcggtct attttttgtt
    10921 ctcagggcag atatcattag gtttaaagat taatctgatc tacccatttg tgggtaaaaa
    10981 tacacataat gcgggtgaca taatagttaa ttaacttttg ttagcgtttt gaaattaaaa
    11041 acaccgttca cctgaagaga tattaatttt tagcgatgat ggagggataa ttatatttga
    11101 tctggcacaa gttttactga tgaaggatgt aacttgtgcc aggggtattt tgcattacgg
    11161 taataattat tacttacaga taaccagcga tccgttattg gcaatattgt ttcagcagtg
    11221 agtagtgttc tgcctgaata cggtaacggt aaaccggacg ccccgtgacg ccataatgga
    11281 tactggtgaa caagatgtgg cagttgacca gccagatgag gtattttcgg caggaaacac
    11341 gcgagatgtt aacctcgtta gccagctcgt cggttgaaaa ctcatagtcc tgatgcgcgt
    11401 caatccactg gcacagcgtg cgtaacgtct gcggcgttaa gccttttggc aagcgacgag
    11461 gatcctgttc gttggagctg ctgccgtgga ttagctgatc aagctcggcc tggtcataat
    11521 actgatgttt ttccagcgcc attttctttt gccgccagcc ggtgagcgcc tcttcaaagc
    11581 gggaagcctg gaagggtttg atcaggtaat ccacgacacc gtaatgcagc gaatctttaa
    11641 tggttgccgc atcggctgcg gaggagatga caatcacatc acttttgcaa cgcgcgttat
    11701 gcaggacagg cagtaaatcg agcccgttct ctttttgcat atagatatcg agcaatatca
    11761 ggtcgatagg cgtatcgcta ttgaagataa tctctttggc tttctccagc gtcgaggctg
    11821 ttccacagca ttgaaagcct gggatttgtg ctacgtatcg gcgattcagc tccgcgacca
    11881 ttgcgtcgtc atcgataatt aatacattga tcatctgttc gacctctccc cgtcccaggg
    11941 tatctggaca aaaaattgtg tgaaaatccc gggttccgat tccacggcga tgctgccgcc
    12001 gagattttct acctgttgtt tgacaagtgc taaaccgacg cctcgctcgc ttccttttgt
    12061 cgagacacct ttgtcaaaaa tgtgatcgat tttgtcgggt gcgatccccg gtccatcatc
    12121 gttaacttcg cagtgcagcc agccgtgacg gtagtgcaat gttacgctaa tttcgccacc
    12181 gggttccggc cctaatgctt ccagcgcgtt ttctatcaga tttcccaatg tggtaatcag
    12241 cgtcgcgacc tggtcctcac tgccgctgtc tggcagcagg ctttcactgt ttaaaatcag
    12301 cgtatggcct aaatcggtcg cgcggttaat cttgctgatt aaaaaaccag cgataaccgg
    12361 agatttgatc ttacccagca gagagccaat ctcttcctga tagttattgg ctgttttgag
    12421 aatgtaatct tccaactgct tataactctt cagatgcaat aatccgagaa tcacatgcaa
    12481 tttattcata aattcgtggg atcgttcacg aagtgcatca gcatagttga ccagaccgtc
    12541 gagtcgctgc atcagtttac gaacttcagt tttgtccctg aaggttgaaa tggcaccgat
    12601 gataacgcca ttactgcgca ccggaacggt gttgatcagt aatagccggt ctttaatcgt
    12661 aatctcttcg tcgcggcgcg gcgtaccgtc gcgtaacact tccgagacat ctaccacctg
    12721 tgaccatgag tggcttagcg tcgacagttt ctcatcgtcc tgcgacttac ggtaattcag
    12781 caattcttgt gcggcatcgt tgatcagcgt gacctcgccg cgatcgtcca cggcaacgac
    12841 gccttctttg atagactgca acatggcctg gcgctgctca aacagcgtgg agatttcgta
    12901 gggttccagg ccgaaaagga tttttttcag taccttaacc agaatgcagg tgccaatcag
    12961 tccgaccagc atgccaaata ataccgacca gataatgctc cagcgactgt cattgatttg
    13021 ttgggtcacg cggcttaact caaggccgat cgccaccacg ccaatttgtt tatgattttc
    13081 atcgtagatg ggggtaaata cgcgtaaagc ctgcgccaga aaaccgcgat tgatagcgac
    13141 attttctttg ccattcagcg ctttaaggat gtcatcacct ttaaatggct gaccaatacg
    13201 ctgggcttca ggatgcgagt agcgaagact ttgcatatcg gtaacgacaa taaacagcag
    13261 atcgttgcgt ttgcgcacgg cttccgcgat ggcctggatt ccgctctcct gcggtttttt
    13321 ctgcaagccc tgacggattt ccggcgagtc ggcgagggta cgcgccactg ccagtgcctt
    13381 gttggccagc ccgtctcgcg tcatatcact gatttgcgag aagtaaatca gatgcaccac
    13441 caatagcacc gagaacagta ccgcgctgac cattaagatc actgtggtac tcaatttcat
    13501 cggacgtttg cgtaacatgc ggtagggcaa tgaatgtctc atcagcttcc ttgtgtgaca
    13561 aatttcttaa gcattatctc tgatgaggcg ggtaattcaa agggagtaag aatgattggc
    13621 tatatagggg aagagactct ggcaacggaa actgccagtg ctgtatgaag attccagggc
    13681 tatgcttata gcgataatca tactgatgag agagggaaag tcatggatca ggcgctactg
    13741 gacgagggtt atcgctgtta taccggcgaa aagatcgatg tctatttcaa cactgcgata
    13801 tgtcagcatt ctggcaattg cgtacgtggc aacggcaagt tatttaatct caaacgaaag
    13861 ccgtggatca tgccggatga agtcgacgtc gtcactgtgg ttaaagtgat tgatacgtgc
    13921 ccgagcggcg cgctgaaata ccgtcataaa taagcgaggg taaaatggaa atacgcgaag
    13981 gccacaataa attttacatt aatgacgaac agggcaagca aatcgctgaa attgtctttg
    14041 tgccgaccgg agagaattta gcgattatcg aacataccga tgtcgatgaa agcctgaaag
    14101 ggcaggggat tggtaaacag ctggttgcga aagtcgtgga aaaaatgcgt cgggaaaaac
    14161 gaaaaattat cccactatgc ccatttgcga aacacgaatt tgataaaacg cgggagtatg
    14221 atgatattcg cagttgatgg gagagtacag agtcacgata tttttcatgc tctccgcgga
    14281 gtgatgcagg agagcatctg aagggtaggg ggatgcacaa agaatgggca gagagcgcgt
    14341 ttttttgtcc caagtcatcc cctttactga gcaaaaaaaa gaatatctcc tatatgagaa
    14401 tcatcaatcg gggttaataa tttttgcgtc cccagagcat tcaatattga taggggtaat
    14461 gactccaact tattgatagt gttttatgtt cagataatgc ccgatgactt tgtcatgcag
    14521 ctccaccgat tttgagaacg acagcgactt ccgtcccagc cgtgccaggt gctgcctcag
    14581 attcaggtta tgccgctcaa ttcgctgcgt atatcgcttg ctgattacgt gcagctttcc
    14641 cttcaggcgg gattcataca gcggccagcc atccgtcatc catatcacca cgtcaaaggg
    14701 tgacagcagg ctcataagaa gccccagcgt cgccatagtg cgttcaccga atacgtgcgc
    14761 aacaaccgtc ttccggagcc tgtcatacgc gtaaaacagc cagcgctggc gcgatttagc
    14821 cccgacgtat ccccactgtt cgtccatttc cgcgcagacg atgacgtcac tgcccggctg
    14881 tatgcgcgag gttaccgact gcggcctgag ttttttaaat ggcggaaaat cgtgttgagg
    14941 ccaacgccca taatgcgggc ggttgcccgg catccaacgc cattcatggc catatcaatg
    15001 attttctggt gcgtaccggg ttgagaagcg gtgtaagtga actgcagttg ccatgtttta
    15061 cggcagtgag agcagagata gcgctgatgt ccggcagtgc ttttgccgtt acgcaccacc
    15121 ccgtcagtag ctgaacagga gggacagctg atagaaacag aagccactgg agcacctcaa
    15181 aaacaccatc atacactaaa tcagtaagtt ggcagcatca tccacctaag taagagaaca
    15241 atgaacagat attaataatt ttgccgcttt tctgcgggat cataattttt gccgcttcat
    15301 agcttaactc gaatgcggcg gtcaggttca catcaatcat cggatcccag tcggcacgac
    15361 cgaagtccag caccttattc agcttacaaa taccggcatt gtttaccaga atatcaactg
    15421 taccgaaacg ctcacagcaa gcagcgataa tcttctgcgg cgcgccttct gcggtgatat
    15481 ccacctgcat gaagtccacc tcaacaccct gtttttcaat catttccttt gtttcgccgt
    15541 tatctttgac gaaactagga ataaagatat ttgcgccagc tttggccaac gccatggcaa
    15601 atgcctggcc taaaccgcta ttcccaccgg taacaattgc ggttttacct ttcagtgaga
    15661 aaaaatccat tgagaacgca ttgagagatt cgattgacat agttagctcc aatttccgta
    15721 aggcaaaaaa aaagaaaggt aacttccccc gcgagaggga agttaccttt ctcattatct
    15781 ctggtaactg gagataattt ttagcataac ggcataactg ctaatgtgat cgtaatcaca
    15841 gtgtgatatt cgtattgtca aatagtttta aaaagcagaa gcgtgattac tctcacacaa
    15901 cacctctgct ttccatgtta tttcttattg caattactag agacctgaga aaagtaatcc
    15961 acctgaaagg gcggcttact tttctctttt ttttgtctat tacaaatggg aatacgatat
    16021 gcaacacaac tcatatcgcc gttggataac cctcgcgata attagtttta gcggcggcgt
    16081 tagttttgac ctggcttatt tacgttatat ttatcaaatt cccatggcga aatttatggg
    16141 attcagcaat accgagatag gtttaataat gagtaccttt ggtattgcgg ctattattct
    16201 ttatgccccc agcggcgtta ttgccgataa attttcacac cgcaaaatga ttacttccgc
    16261 gatgatcatt accggattac tgggtctgtt aatggcaacg tatccaccgc tgtgggtaat
    16321 gctctgtatt caggtcgcct ttgcgataac gacgatttta atgctgtggt cggtgtcgat
    16381 taaagccgcc tcgttgctgg gcgatcatag tgagcaaggg aaaattatgg gctggatgga
    16441 agggctgcgc ggcgtcggtg taatgtcgct ggcggtgttt accatgtggg tcttttctcg
    16501 ctttgcaccg gatgacagcg ccagcctgaa aacagtcatt atcatctaca gtgtggttta
    16561 catcttgttg gggattctgt gctggttttt tgttagcgat aacaacaacc tgcgcagtgc
    16621 caataacgaa gaaaaacagt cattccagct tagcgacatc cttgccgttt tgcgtatcag
    16681 caccacctgg tattgcagca tggtgatttt tggtgtcttc acaatctacg ccattctgag
    16741 ttactccacc aactatctga ccgaaatgta cggcatgtcg ctggtggcgg cgagctacat
    16801 ggggattgtg atcaacaaaa tcttccgcgc gctgtgcggc ccacttggcg gcattatcac
    16861 cacctacagt aaagtgaaat cccctacccg cgtgatccaa atcctttccg tactcggcct
    16921 gctgacgtta actgccctgc tcgtcacgaa ctctaacccg caatcggtcg cgatggggat
    16981 tggcctgatt ttactgctgg gattcacctg ttacgcctca cgcgggctgt actgggcctg
    17041 ccctggcgaa gcgagaacac cgtcttacat tatgggcacc acggtaggta tttgttcggt
    17101 gattggattc ctgccggatg tcttcgttta cccaattatc ggccactggc aagacaccct
    17161 gcccgctgca gaagcctacc gcaatatgtg gctgatgggc atggcggcgc ttggcatggt
    17221 gattgtcttt acctttttgc tgttccaaaa aattcgtact gctgatagcg cccccgcaat
    17281 ggctagcagc aagtaagcca agaaaattac aggagaaaaa tcatgtcgaa aaaatacatc
    17341 atagggatta atggcggaag tcagagcaca aaagtggtga tgtacgatct ggaaggtaac
    17401 gtggtttgcg aaggcaaagg cttattacag ccgatgcaca cgccagatgc cgatactgca
    17461 gaacatcctg acgacgattt atgggcatca ttatgttttg ccggtcacga tttgatgagt
    17521 cagtttgccg ggaataaaga agatattgtc ggtattggtc tgggatccat ccgttgctgt
    17581 cgtgcgttat tgaaagccga tggcacgcct gctgcgccgt tgattagctg gcaggatgca
    17641 cgcgttacac gcccttatga acacactaat cctgacgtgg catatgtcac ctctttttcg
    17701 ggttatctga cgcatcgctt aaccggcgag tttaaagaca atatcgccaa ctattttggt
    17761 cagtggccgg tggattataa gagctggaca tggagcgaag atgctgcggt aatggataag
    17821 tttaatatcc cccgtcatat gctgtttgat gtgcaaatgc ctggcaccgt cctcggacat
    17881 atcacaccac aagccgcact ggcgacacat ttcccggcag gactgccggt tgtttgtacc
    17941 accagtgata aaccggtaga agctctgggg gccggattac tggatgatga aactgcggta
    18001 atttctcttg gcacttacat cgcattgatg atgaacggca aagcactgcc gaaagatccg
    18061 gtggcgtact ggccgattat gtcttctatt ccgcaaacat tgctgtatga aggttacggt
    18121 attcgcaaag gtatgtggac ggtgagctgg ctgcgtgaca tgttaggcga gtcgttaatt
    18181 caggatgcca gggcgcagga tctttcaccg gaagatttac tcaacaagaa agcctctagt
    18241 gtgccacctg gctgtaatgg gctgatgacg gtgctggact ggctgaccaa tccgtgggaa
    18301 ccgtacaaac gcgggattat gatcggcttt gattccagca tggattacgc atggatatat
    18361 cgttcgatat tggaaagcgt ggcgctaacg ctgaagaaca attacgacaa tatgtgtaat
    18421 gaaatgaatc actttgcgaa gcatgtgatc attactggcg gcggttcgaa cagcgatctg
    18481 tttatgcaaa tttttgccga cgtgttcaac cttccggcac gccgtaacgc cattaacggt
    18541 tgtgcaagtc tgggagcagc gataaataca gcggtaggtc tggggctata cccggattac
    18601 gcaacggctg ttgataagat ggttcgcgtg aaagatatct ttataccgat tgagagcaat
    18661 gccaaacgct acgacgcgat gaataaaggc attttcaaag acctaaccaa acatactgat
    18721 gtgatcctga aaaaatcgta tgaagtgatg catggggaat tggggaatgt ggattcgatc
    18781 cagagctggt cgaatgcgta agtgaggaag gccgggcggg aaactgcccg gcctgaacat
    18841 acctgaatgg ttatccccgc tgacgcgggg aacataagtt cgacaactta aaaaatgata
    18901 acaagtccgg tttatccccg ctgatgcagg gaacataata aaaactttaa ctagttcggt
    18961 ttatccctgt ttttacaagg aatattaact acactcgatg tctttagaat aaaagacaat
    19021 acgaatcgca ctttatca
//
LOCUS       DAZKIV010000087        18843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZKIV010000087 DAZKIV010000000
VERSION     DAZKIV010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.087"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            268..768
                     /gene="tssB"
                     /locus_tag="ACHOT6_002952"
     CDS             268..768
                     /gene="tssB"
                     /locus_tag="ACHOT6_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFP9948132.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDVQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            803..1027
                     /locus_tag="ACHOT6_002953"
     CDS             803..1027
                     /locus_tag="ACHOT6_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948133.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1078..2553
                     /gene="tssC"
                     /locus_tag="ACHOT6_002954"
     CDS             1078..2553
                     /gene="tssC"
                     /locus_tag="ACHOT6_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFP9948134.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2560..2973
                     /gene="tssE"
                     /locus_tag="ACHOT6_002955"
     CDS             2560..2973
                     /gene="tssE"
                     /locus_tag="ACHOT6_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFP9948135.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2977..4827
                     /gene="tssF"
                     /locus_tag="ACHOT6_002956"
     CDS             2977..4827
                     /gene="tssF"
                     /locus_tag="ACHOT6_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFP9948136.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4791..5873
                     /gene="tssG"
                     /locus_tag="ACHOT6_002957"
     CDS             4791..5873
                     /gene="tssG"
                     /locus_tag="ACHOT6_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFP9948137.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5898..7178
                     /gene="tagH"
                     /locus_tag="ACHOT6_002958"
     CDS             5898..7178
                     /gene="tagH"
                     /locus_tag="ACHOT6_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFP9948138.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7175..7699
                     /gene="tssJ"
                     /locus_tag="ACHOT6_002959"
     CDS             7175..7699
                     /gene="tssJ"
                     /locus_tag="ACHOT6_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFP9948139.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7702..9033
                     /gene="tssK"
                     /locus_tag="ACHOT6_002960"
     CDS             7702..9033
                     /gene="tssK"
                     /locus_tag="ACHOT6_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFP9948140.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQHFPDERSRNIC
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDTRTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHHARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANMPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRALMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            9038..9799
                     /gene="icmH"
                     /locus_tag="ACHOT6_002961"
     CDS             9038..9799
                     /gene="icmH"
                     /locus_tag="ACHOT6_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFP9948141.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9808..12591
                     /gene="tssH"
                     /locus_tag="ACHOT6_002962"
                     /gene_synonym="clpV"
     CDS             9808..12591
                     /gene="tssH"
                     /locus_tag="ACHOT6_002962"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFP9948142.1"
                     /translation="MIQIDLPTLVKRLNLFSRQALEMAASECMSQQAAEITVSHVLIQ
                     MLAMPRSDLRVITRQGDIGMEELRQALTVENYTTARSADSYPAFSPMLVEWLKEGWLL
                     ASAEMQHSELRGGVLLLALLHSPLRYIPPAAARLLTGINRDRLQQDFVQWTQESAESV
                     VPDADGKGAGTLTDAADTLLARYAKNMTAEARNGRLDPVLCRDHEIDLMIDILCRRRK
                     NNPVVVGEAGVGKSALIEGLALRIVAGQVPDKLKNTDIMPLDLGALQAGASVKGEFEK
                     RFKGLMAEVISSPVPVILFIDEAHTLIGAGNQQGGLDISNLLKPALARGELKTIAATT
                     WSEYKKYFEKDAALSRRFQLVKVSEPNAAEATIILRGLSAVYEQSHGVLIDDDALQAA
                     ATLSERYLSGRQLPDKAINVLDTACARVAINLSSPPKQISALTTLSHQQEAEIRQLER
                     ELRIGLRTDTSRMTEVLVQYDETLTALDELEAAWHQQQTLVREIIALRQQLLGVAEDD
                     AAPLPDADTVEDTQPESESESESESEQDNTGAVPADEADREQPEETAETVSPVQRLAQ
                     LTAELDALHNDRLLVSPHVDKKQIAAVIAEWTGVPLNRLSQNEMSVITDLTKWLGDTI
                     KGQDLAIASLHKHLLTARADLRRPGRPLGAFLLAGPSGVGKTETVLQLAELLYGGRQY
                     LTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGVLTEAIRQKPYSVVLLDEVEKAHPDV
                     LNLFYQAFDKGEMADGEGRLIDCKNIVFFLTSNLGYQVIVEHAYDPETMQEVLYPVLA
                     DFFKPALLARMEVVPYLPLSKETLATIIAGKLARLDNVLRSRFGAEVIIEPEVTDEIM
                     SRVTRAENGARMLESVIDGNMLPPLSLLLLQKMAANTAIARIRLSAVDGAFTADVEDA
                     QNDESVTKDETVL"
     gene            12588..13331
                     /gene="vasI"
                     /locus_tag="ACHOT6_002963"
     CDS             12588..13331
                     /gene="vasI"
                     /locus_tag="ACHOT6_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFP9948143.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13336..14748
                     /gene="tssA"
                     /locus_tag="ACHOT6_002964"
     CDS             13336..14748
                     /gene="tssA"
                     /locus_tag="ACHOT6_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFP9948144.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14857..18291
                     /gene="tssM"
                     /locus_tag="ACHOT6_002965"
     CDS             14857..18291
                     /gene="tssM"
                     /locus_tag="ACHOT6_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFP9948145.1"
                     /translation="MLILAWIFLLVWIWWKGPMWTLYEEQWLKPLANRWLATAAWGII
                     ALVWLTVRVMKRLQQLEKMQKQQREEAVDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGARGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHALGWLKEKRARQPLNGIILTLDLPDLLTADKRRREHLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRAWLIRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGEDDFGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWTPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLLRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNKQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNNVIIREDIIAQLETAQKIRDIFFRKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDGGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18302..>18843
                     /locus_tag="ACHOT6_002966"
     CDS             18302..>18843
                     /locus_tag="ACHOT6_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HFP9948146.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQNTRKRLDEPPRKS"
BASE COUNT         4359 a         5223 c         5245 g         4016 t
ORIGIN      
        1 cataaaaact ctatatttaa attattgcca taatacacct gacgcgaatt gcacaggttc
       61 acacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaaa agtccttttc
      121 ttatttattt tgaagcgtaa aatgcggggt gatgaaggag tggtgatttt attttcaccg
      181 ccattagggc ggcattgccg atattttttt gcggtacaaa tatgccgtgt tcatggcctg
      241 tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag cgtcgcgcct
      301 cgcgagcgta ttaatatatc gtatgtccca aaaacagatg tccagacggc tgaagtggaa
      361 cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggaaac atccccactg
      421 gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtgat ggctgaagca
      481 gccattggcc tcaattttac tgtccccgcc acgctgaagg gaagcacaac agacgatgaa
      541 ctgaatgtgg cgctgaatat caaatcactg gatgattttt caccagatag cgtggcccgg
      601 caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc actgaagggg
      661 ccgatgggta accttccggc attccgtacc cagctccagg cattgctgga aaatgaagag
      721 tcccgcgagc agttgctcaa agaaatcggt ctggtgagca ataaataacc accactattt
      781 cattaaacag ggttatccag gcatgacggc acacaaaaat atctccggga cataccacct
      841 ctctgttgca gacattctgc aggtggttta ccaggtatgt ttcagcccgt ccgttgaaat
      901 caaccaggat ggtgtggctg cgctgattac aacactggat cgccgtatca gtgatctatt
      961 ggatgagatt atccattttt gtgagtttca gcagagcgcg tcgcactggc agcgcgtact
     1021 tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa acagaaggat tttcagtatg
     1081 tctttacagg aagaggaact cgtttccagt catgccgggc agcctgagca ggcatcgtcg
     1141 ctgcttgatc aaatcatggc acagacccgt attcagcccg gttcggaagg ttatgacgtt
     1201 gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc atctgccgaa
     1261 ccggtcaata aactggccgt ggacagcatg attgcagata ttgatgaacg catcagccgc
     1321 cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgagtcgtt ctggcgctca
     1381 ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa cgtgcttcat
     1441 gtcaccaagc aggaacttct ggaagacttt gagtttgcgc cagaaattat tcagtccgga
     1501 ttttataagc acgtttattc gtccggtttc ggacagtttg gcggtgaacc catcgctgcc
     1561 gtgctgggcg cttatgagtt caaaaatacc gcgcctgaca tgaaactgct gcaatacgtc
     1621 agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc ggagtttatg
     1681 gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat ctttgaaggt
     1741 ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta tttagggctt
     1801 acggcacccc gcttcctgct tcgccagccc tattcaccaa cagataaccc tgttaagaac
     1861 tttaactatt acgaagatgt cagccagaac cacgaagatt atctgtgggg taatacggcc
     1921 tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg tccaaacatt
     1981 atcggtccgc aaagcggcgg cgcagtgaaa gatctgccgg tgcatctgtt cgaaacgatg
     2041 gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg cgaatttgaa
     2101 ctggctgaag agggttttat cacccttacc atgcgtaaag actctgataa tgcagccttt
     2161 ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagatgc agaaaccaac
     2221 tataagctgg gtacgcagct tccttatctc ttcatcatca atcggttagc gcactacatc
     2281 aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt agaaagagag
     2341 cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc agacgtgcgc
     2401 agccgcaaac ctctgcgcgc tgccaaagtt gaggttatgg atgtagaagg cgaacccggc
     2461 tggtaccagg ttgcgctaag cgtgaggcct cattttaaat ttatgggggc aaattttgag
     2521 ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga tcgggaaaga
     2581 accacaggtt ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg gcagggaggc
     2641 agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct taatacccgg
     2701 tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg ggctgaaaac
     2761 ttccgcgaat ccatgagtcg ggccatcgag caatgtatag aacgctatga accccggatc
     2821 tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga tatgacgttt
     2881 catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt tgatatggca
     2941 ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga acgctatttc
     3001 cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga aaaaccccat
     3061 ctggcccgct tcctggccga aaaagatgcg gatccggata ttgaacgcct gctggaaggg
     3121 gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc agaactgacg
     3181 cacgggctta ttaagatgct atggcctaat tacctgcgtc cggttccggc aatgaccctt
     3241 attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc ccgtaatgag
     3301 cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc tgatgctaaa
     3361 aaggaggagc cgcctccctg taccttcacc ctctgccggg atatctggct gctgcccgtt
     3421 cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa catcaccttt
     3481 tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg cttctggctc
     3541 ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga atacttgcag
     3601 ggtgccgacc tgactgtggg tgaacagcat attcgcctgc ctgagtttat gctaaaagct
     3661 gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca cagcggctac
     3721 cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga tctttgtggt
     3781 tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact gcgtttttcg
     3841 cgccctttgc ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta ttgcgcacct
     3901 gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg gcgggcagac
     3961 tatccgctgg ttcccagccg ccattaccca caacattacg atgtattttc cgttaacagt
     4021 gtggtgagcc aggtccagga tatgttcagg aaaaaagatc tggggcgtcc tgtttcgacg
     4081 caggccgcgc gccagtggcc agcctttgaa agtttcagcc atcagatgga atacagccgg
     4141 aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca tcgcggcttt
     4201 gatcataccc ttgcctttat acatgctgat ggcagttatc cgtcagacga atctctgctc
     4261 agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc gtcacaaatt
     4321 cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc atttcgcaac
     4381 attacccgcc cgacgcaacc actctggccg gtcattgatg gcagcctgca ctggtcccta
     4441 ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct gaagcaggtc
     4501 atcgccaact ttgatcgcca cgcaatccat catccgcaga cggcacggct gtcacaacaa
     4561 aagctggatg ccattgagcg tctggagacc cgccccgttg atcgcctgtt tacgggtatt
     4621 cccgtccggg gactggcctc cacgctgtat ctgcacccgg agccgtttgt ctgtgaaggg
     4681 gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc cagcgttaac
     4741 tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa atggacggaa
     4801 agaatcgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat aaagagcaga
     4861 atgatccgct ggcacaggtt ttccataatg cctgctctta caattttttt gcgatggcgg
     4921 agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc ctgcgtgacg
     4981 atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc ccgtgcagtg
     5041 atatcagtgc gctgaaacgg gatacgtcag gggcattcag gatgaccacc acctttatgg
     5101 ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg gcctggaaag
     5161 cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac cgcctgaccc
     5221 agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt aacggcggtg
     5281 tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc cagcttcgcg
     5341 ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg gcaaatccgg
     5401 gacgatcccc ggaaataatc tgtggcctgg tgtcccactg ctttgatttg tcagaagtga
     5461 cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa aatagtctgg
     5521 ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg ggcaactttg
     5581 ttctgggtac acgagtgccc gatctcagcg gaaaattcca gctcagtatt accagcctga
     5641 cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca ctgacgatgt
     5701 tcgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg ctggcgccgg
     5761 agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc tggaccagct
     5821 tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg taaattatga
     5881 taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg caggtcatta
     5941 acggcagtga gctggaaagc ggacgggcgg cgcgctgtct gttcacacag cagggaaatg
     6001 tcggccatgg ccccgaatgc cactggtcgg tacaggatcg tcagcagagc attccggccc
     6061 aggcttttac cgttatcctg cacgatggca cattttgtct gcgcccgcag acggcacaac
     6121 tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct aatacagttg cgccagggcg
     6181 atgagatcca gatcggacgg ctgatggtga gggttcatct gaaccgggga gatattcccc
     6241 attacgatga ggaaatggcc actcccgaaa ccatcgttac caatcgcgat atgctcacgg
     6301 ataccctgct atcaacggag ggtacgccac actatccggg aatgactcac cggcaccagc
     6361 ttgcagacac cgtggtaaac ggtttttctg ccgatccact ccaggcactt cagtccgaaa
     6421 gcctgattac cacgggcgat ccgctttcag gcattgcggc tgtccggcca tcggcaccgc
     6481 tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg ccgcccattt
     6541 atgccagccc cggcgatcgt aatgacgacg tctctgcggc agaaatggcg caacgccacc
     6601 ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccatgagc aattccgacg
     6661 atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata aaaggtctgc
     6721 tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg ctggaagata
     6781 acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtaatgttt gccgaaggta
     6841 aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc aatgtccgcc
     6901 accacgaaga agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg ctggatgctt
     6961 tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat gaactgcgcc
     7021 agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa gaactggcct
     7081 ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag gtctatgacc
     7141 gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt ttctggcctg
     7201 cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca gtgaggtgat
     7261 caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa taaccgtcac
     7321 cctgctgacc gaaccggaca ccaataccaa tgcggaaggt gaatcagccg ccgtggatgt
     7381 gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg accagatagc
     7441 cagtacgccg ctaccggatg tgctgggtaa aaactatatc gaccaccagg atttcaacct
     7501 gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa cgcagtttat
     7561 tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa tcgagacggt
     7621 ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca gcatcgaaat
     7681 gaaaaaagag gatgaataat tatggcaact acccgcaaca aggtgatgtg gcaggaaggg
     7741 atgctgatgc gtccacacca tttccagcag cagcagcgtt acaacgacta cctggataac
     7801 cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac tctcaacaat
     7861 gaactgctgg cacagggtaa gatcatgatt gacagcgcgt caggcacact gcccgacggc
     7921 accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc gcaacatttt
     7981 ccggacgaga gaagccgcaa tatctgcctc gctctgccgg tcgccagtga tgtgagaaat
     8041 gaaatcagcg acgggcggcg aatcgggcgt taccggctga attatgccga tgtccgggat
     8101 ttgcattcag aagaaggtga cacgcgaaca ctgacgctgg gacaactgac gccgcgcatt
     8161 atgagcggtg cagaagatat gagcgcctat attacgctcc cgctttgccg tatcagcgat
     8221 cgccatgccg acggctccct gacgctggat gacgatttta tcccctcctg ccagaatatt
     8281 caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat tggcgggcgg
     8341 gcaagcgatc tggcaaaccg cattggctct ccggcgcaga gcggcatcgc ggatgtggcg
     8401 gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca tcacgcccgt
     8461 cgatcccagc tccacccgga agatttctac cttgatcttg ccgggttgct gggtgaactg
     8521 atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga tcatcatgac
     8581 ctgaccaaaa catttaaaac gctgttaccg gaagtcaaac gggcgctgca taccgtactg
     8641 tcgccaagag ccgtcaatct gccgctgcat ctgcgtgacg gtatctggca ggccgatatc
     8701 catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc aaacatgccg
     8761 gtcgatcaga tccagcgcca gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc
     8821 cgcaatatgg tcagtgtgca gatccccggc attccattgc gcgcactgat ggtggctccc
     8881 cgccagcttc cttaccattc cgggttcagc tatttcgaac tcgacaagag cggacaggcc
     8941 tggacagaaa tggctgccgc cggggccgtt gcactgcatg tatccggcag tttcccggat
     9001 ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt cactgtctct
     9061 gccaccattt accggatacg acgaaaaaag ccagcgcaac taccatctcg cgcttcgtgg
     9121 caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg taatgcgcct
     9181 ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg tgacggatgt
     9241 tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga ttatctcgtt
     9301 tcgctacatc ctctgcacgt ttattgatga agcggcgctc ggtaacggct ggtcaaacaa
     9361 gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct ggggtggaga
     9421 gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc aggatctgct
     9481 ggaatttctc tggatctgtt tttctctggg atttcgcggg cgctacaaag tggcagcaca
     9541 ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc ataaactgcg
     9601 cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg ggcgttatca
     9661 gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt
     9721 gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc tgcaccagct
     9781 aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac gctggtaaaa
     9841 cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg tatgagtcag
     9901 caggcagcgg aaattaccgt cagccatgtg ctcattcaga tgctcgccat gccacgcagt
     9961 gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg ccaggcgctg
    10021 acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt ttccccgatg
    10081 ctggttgagt ggcttaaaga gggctggctg ctggcgtcgg ctgagatgca gcacagcgaa
    10141 ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta tataccgcct
    10201 gctgccgccc ggctgttgac cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag
    10261 tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca
    10321 ctgacggacg ctgctgacac cctgcttgcc cgctacgcca aaaacatgac cgcagaggcc
    10381 cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct gatgatcgac
    10441 attctctgcc gccgccgcaa gaacaacccg gtggtggtgg gcgaagcagg cgtgggtaaa
    10501 agcgccttaa tcgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaagctg
    10561 aaaaacaccg atatcatgcc ccttgacctg ggcgcattgc aggccggagc gtcggtgaag
    10621 ggtgaattcg aaaaacgttt caaagggctg atggcggagg tcatttcctc cccggtgccg
    10681 gtcattctgt ttatcgacga agcacatacc ctgattggcg cgggcaacca gcagggcggg
    10741 ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa aaccatcgcc
    10801 gccaccacct ggagcgagta caaaaaatac ttcgaaaaag atgccgccct gtcgcgccgc
    10861 ttccagttgg tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgcggt
    10921 ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc cttgcaggcc
    10981 gctgcgacat taagcgagcg ttatctctcc gggcgtcagt taccggacaa agcgattaat
    11041 gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc
    11101 tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag
    11161 cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat
    11221 gaaacgctga cggcgctgga tgaactggaa gcggcctggc accagcagca gacgctggtc
    11281 cgggagatta ttgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg
    11341 ttgccggacg cagataccgt ggaggatacg cagccagagt cagagtcaga gtcagagtca
    11401 gagtcagaac aggataatac cggtgccgta ccggctgatg aggccgacag agaacaaccg
    11461 gaagagaccg ctgaaacagt ttccccggta cagcggctgg cccagctcac tgccgaactg
    11521 gacgccctgc ataacgaccg gttgctggtc tccccgcacg tcgataaaaa acagattgcg
    11581 gcggtgattg ccgaatggac cggcgtgccg ctcaaccgcc tgtcgcagaa tgaaatgtcg
    11641 gtcatcaccg acctgacgaa atggctgggt gacaccatca aaggccagga cctggcgatt
    11701 gccagcctgc ataagcatct actgaccgca cgcgccgacc tgcgtcgtcc gggacgcccg
    11761 ctcggtgcgt tcctgctggc tggccccagc ggtgtgggta aaaccgaaac cgtcctgcaa
    11821 ctggcagagc tgctctacgg cggtcgccag tacctgacca ccatcaatat gtccgaattc
    11881 caggagaaac acaccgtctc gcggctgatt ggttcgcctc cgggctacgt tggctacggt
    11941 gaaggcggcg tactaaccga agcgattcgc cagaaaccct actcggtagt actgctcgat
    12001 gaagtggaaa aagcgcaccc ggatgtgctc aacctgttct accaggcgtt cgataagggc
    12061 gaaatggcag acggtgaagg ccgcctgatt gactgcaaaa atatcgtctt cttcctgacg
    12121 tccaacctcg gctaccaggt aatagtcgag catgcctatg acccggaaac catgcaggaa
    12181 gtactgtatc cggtgctggc cgacttcttc aaacctgccc tgctggcgcg tatggaagtg
    12241 gtgccgtacc tgccgctgtc gaaagagacg ctcgccacca tcattgccgg gaaactggcc
    12301 cgcctggata acgtgctgcg cagccgcttt ggcgcggagg tgattataga accggaagtg
    12361 acggacgaaa tcatgagccg cgtcacccgc gcggaaaacg gcgcgaggat gctggaatcg
    12421 gtcatcgacg gcaatatgct gccgccgctc tccctgctgc tgttgcagaa aatggcggcg
    12481 aatacggcga ttgcccggat tcggttgtcg gcagtggacg gcgcatttac ggcagacgtg
    12541 gaagatgctc agaacgacga gtccgtcaca aaggatgaaa cggttttatg agcaggttgc
    12601 gcgttttccg gcagacagga tggtttatcg cgggcctgat gacgggctta cccgccactg
    12661 ccgcgcccgc agaagcatcg tcaatggccg gtgtggcggt cgccgtcgcc accacgaccc
    12721 cgccggatgc gacagccacg ttacaggcca tgcagtcctg tcggcgggag tccgcggcgc
    12781 tggagcggct tgactgctat gaccgcctgc tggcaccgct gtccccgtca gggtttgacg
    12841 gggcgctggt caaagccggt tttgtgggcg aggcctggac ccgcgccaca gagcaggaaa
    12901 aacgccgcga gggcaacacc acagaactgc tggtgacgca ggtgccgggg gagcgcccga
    12961 cggtggtgat aaccaccccg gctatcggtc atgttccgcc acgcccggtg ctgatgttca
    13021 gttgcgtgga caatatcacc cggatgcagg tggcgctgat gcaccctctg gatgtccatg
    13081 atatcgccgt cactctgaat gccgacaacc gggcattgcg cagccactgg tttgtgcgcg
    13141 agaacggcac cctgctagag tccagccggg ggttatccgg gattgatgaa attaagcaac
    13201 tgttcggcgc aaaaacgctg acggtggata ccggcgcaga caatgccgcc ggaaagctga
    13261 cctttaacat tgacgggctg gcaagggcga ttgctcccct gcgcgatgcc tgccactggg
    13321 cgggagaata aaaatatggc aatggactta cgcgatccga atgtctggat atcgcacctg
    13381 ctggaaaacc tgccggaaga gaaactggca tcggcgctga aagatgacaa cccgaactgg
    13441 gagtatatcg acggcgaaat cgtcaagctg ggatcgctgg cccacagtca actggatatc
    13501 ccggaactcc agcgccgggg gctggtcatt ctggcttcgg agagtaagga ttttcgtctg
    13561 ctggcgcatc tgctgagaac cctgcaacac gccggtgatc ctcatctcgc attgcgcctg
    13621 ctggcgctgt atgtggagca ttactgggcg gtggctgcgc cgcagaatat ggcgcataaa
    13681 aaacgctttg ccagccaggt cattaaacgc tttgaaacag gtattgaagt tttttcacag
    13741 aacgccgcca cagcgcagcg cgatgcgctg tcaggggaac tggcaaaact ggcgcaatgc
    13801 tggcagtcac ataacgcccc ggaactggca caggctactg atgatctttt tgccctgtac
    13861 cagcgtgcgt ttaatcgtgc ggcaccggct cctgtcccca ctccggcggc ctccggtagt
    13921 tcaccacaga ccaccgccac gtctgaaagc ggcgtgacgc aacccagtgc tccggctccc
    13981 caaatcgtca tcgacagtca cgacgacaaa gcctggcgcg acacgctgtt aaaagtggcg
    14041 gccattttat gtgaacgcca gccggattca ccgcagggct accgtctgcg ccgccatgcg
    14101 ctttggcaga acatcaccag tacgccccag gcggaaagcg atggccgtac tccactggct
    14161 gcggtctccg ccgatatggt ggcagattac catgctcagc tcggtagtgc cgatatggcg
    14221 ctgtggcagc aggtggagaa aagcgtactg ctggctcctt actggctgga tggtcactgc
    14281 ctttctgcac agacggcgct gcgtctgggt tacaaacagg tggcagacgc catccgcgat
    14341 gaggtcatcc gcttccttga gcgtctgccc cagcttaccg ggctgctgtt taatgaccac
    14401 acaccgttta tcagtgagca gacgaaacaa tggctggcag cttcgcccga cgccaaagtt
    14461 gcaccggttg cgcaaatcgg tgaggaatcg aaggcagcca gagcctgttt tgctgagcag
    14521 ggtctggagg cggcgctgcg atatctggac atgctacccg aaggcgatcc ccgcgatcag
    14581 tttcaccgcc agtaccttgc cgcacagttg acggaagagg cggggctggt acagcttgcg
    14641 cagcagcagt accggatgtt gttcaggatg ggattgcaga tgatggtggc agactgggag
    14701 ccgtccctgc ttgagcagct agaacaaaaa ttcacggcag aacaataaaa gagaacagga
    14761 gtttttgtgt tcagattacc aacaccccga ctactcagcg gactcaaatc agccctgcga
    14821 ccggcgatgc cccggtttaa agtctctgct ttctggctgc tgatactggc gtggattttt
    14881 ctgctggtgt ggatctggtg gaaaggcccg atgtggacgc tgtatgaaga acagtggctc
    14941 aaaccactgg cgaaccgctg gctggcaacg gcggcgtggg ggattattgc cctggtgtgg
    15001 cttaccgtcc gggtgatgaa gcgcctgcaa cagcttgaaa aaatgcaaaa gcaacagcgc
    15061 gaggaagccg ttgatccgct cagcgtggaa ctgaacgccc agcagcgtta tcttgaccgc
    15121 tggctgctgc gcctgcaacg ccatctcgac aaccgccgtt tcctgtggca gttgccgtgg
    15181 tatatggtca tcggcccggc gggcagtggc aaaacgacac tgctgcgcga ggggtttccg
    15241 tccgacatta tttatgcccc ggagggcgca cgcggcgcag aacaacgcct gtacctcacg
    15301 ccccatgtcg gtaaacaggc ggtgatcttt gatatcgacg gcacactctg cgctcccgct
    15361 gatgcggata tcctgcatcg ccgtctgtgg gaacatgccc tcggctggct gaaagaaaag
    15421 cgcgcgcgcc agccgctgaa cgggattatt ctgacactcg atttacccga tctgcttacc
    15481 gcagacaaac gccgccgcga gcatctgtta cagacgctgc gcagccgctt acaggatata
    15541 cgccagcatc ttcactgcca gttaccggtt tacgtggtac tgacccggct tgatttatta
    15601 cagggttttg ccgccctgtt ccagtcactc aacagacagg atcgcgatgc gattctgggc
    15661 gtcacgttca cccgccgtgc ccatgaaaat gatgactggc gaacagagtt gaatgctttc
    15721 tggcagacat gggtggatcg aatgaatctg gcgttgccgg atctgatggt cgctcagact
    15781 cacacccgcg cgtctttatt cagtttttcc cgccagatgc agggaagccg tgaaccgctg
    15841 gtgtcactgc ttgagggtct gcttgatggc gaaaatatga acgtgatgct gcgtggtgtc
    15901 tatctcacct cttcgcttca gcgtggacag atggatgata tattcaccca gtctgccgcc
    15961 cgccagtacc ggctgggcaa taacccactg gcgtcctggc ccctggtgga caccgcgcct
    16021 tatttcaccc gcagcctgtt cccgcaggca ttactcgcag agcctaatct ggcaacagag
    16081 agccgcgcct ggctgatacg ttcccgtcgc cgcctgacgg ttttctctgc cacaggcggc
    16141 gtggcagcac tgctgctcat caccggctgg catcactatt acaacggtaa ctatcagtcc
    16201 ggcatcaccg tgcttaagca ggccaaagcc tttatggacg tgccgcctcc gcagggggaa
    16261 gatgactttg gcaacctgca actgccgctc ctgaacccgg tacgcgatgc cacactggcc
    16321 tatggcgact ggggcgaccg cagccgtctg gccgatatgg gactgtacca gggacgacgt
    16381 atcgggcctt atgtggaaca gacctatctg caactgctgg agcaacgtta cctgccctcg
    16441 ctgtttaacg ggctggtcaa agcgatgaac gccgcgccgc cggagagtga agaaaaactc
    16501 gcggtgctgc gcgtaatgcg aatgctggag gacaaaagcg gacgtaacaa tgaggtggtg
    16561 aagcagtata tggcaaaacg ctggagcgaa aaattccacg gccagcgcga tatccaggca
    16621 caactgatgt cccatcttga ctacgcgctg gctcatactg actggcatgc agagcgtcag
    16681 gcgggcgacg gtgacgccat cagccgctgg acgccatatg acaagcccgt ggtatcagca
    16741 cagaaagaac tgagcaaact gcctgtctac cagcgggttt accagagcct gaaaacgcgg
    16801 gcgctgggcg ttcttcctgc cgacctcaat ctgcgtgacc aggtagggcc aacctttgac
    16861 caggtgttta catctgccga tgacaacaaa ctggttgttc cacagtttct tacccgttac
    16921 ggcctgcaaa gctattttgt aaaacagcgc gatgaactgg ttgaactgac ggcgatggat
    16981 tcctgggtac ttaaccttac ccgcagcgtg aaatacagtg acgccgaccg cgcggaaatc
    17041 cagcgccagt tgaccgagca gtatatcagc gactacaccg ccacctggcg ggccgggatg
    17101 gacaatctga atatccgcaa ttttgagtcc atcggacaac tgaccggggc gctggagcag
    17161 gttatcagcg gcgaccagcc tttgctgcgg gcgctgaccg tgcttcgtga caacacacag
    17221 ccaggcgtct tttctgaaaa actctctgcc aaagaacggg atgaggccct ggcagaacca
    17281 gactaccagt tgctcacccg tctcgggcat gaattcgccc cggaaaacag taccctggca
    17341 gtacagaaag acaaagaaag cacgatgcag gccgtgtatc agcaactcac cgagttgcac
    17401 cgctatctgc tggcaatcca gaacgcgcct gtaccaggga aatcggcgct gaaagccgtg
    17461 cagttaaggc ttgatcagaa cagcagcgat ccgatattcg ccacccgcca gatggcaaaa
    17521 acactgcccg ctccgctcaa ccgctgggtc ggcaggctgg ctgaccaggc ctggcatgtg
    17581 gtgatggtgg aggccgttca ctatatggaa gtggactggc gcgacagcgt ggtgaaaccg
    17641 tttaacaagc aactggcaaa taactatccg tttaatccgc gttctgcaca ggatgcctca
    17701 ctggatgcct tcgaacgctt ctttaaaccg gatggcatac tggatacctt ctaccagcag
    17761 aacctgaagc tgtttatcga taatgacctg agtctggagg atggcgataa caatgtcatt
    17821 attcgcgaag atattattgc gcaactggaa actgcgcaga aaatccgtga catcttcttc
    17881 cgcaaacaga acggtctggg aacatccttt gccgtggaaa cggtatcgct ttccggcaat
    17941 aaacgccgca gtgtactgaa ccttgacggt cagttagtcg attacagcca gggccgtaac
    18001 tataccgccc atctggtctg gcctaacaac atgcgcgaag gcaacgaaag taagctgacg
    18061 ctcatcggca ccagcggcaa cgcgccgcgc agtatcagct tcagcgggcc gtgggcgcag
    18121 ttccgcctgt tcggggccgg acaactgacc ggagtacagg atggcaactt taccgtgcgc
    18181 tttagcgtgg acggtggcgc gatgacctac cgtgtgcata ccgacacgga agataacccg
    18241 ttcagcggtg ggttgttcag ccagtttggt ctgtcagaca cactgtactg aggaggcagg
    18301 gatgaatagt aacgtactga cacaaactat cgttaccggc agtgacccgc gcgggctgcc
    18361 ggaattcagt gccatccgcg aggaaataaa caaagccagc cacccgtcac agcctgagct
    18421 gaactggaaa ctggtggagt cgctggcgct ggcgattttt aaagccaacg gtgtggattt
    18481 acacaccgcc acctactata cgcttgcccg gacacggaca cagggactgg cgggattctg
    18541 cgaaggtgcg gaactgctgg cggcaatggt aagccacgac tgggataagt tctggccgca
    18601 gggcggcccg gcgcgtactg aaatgctgga ctggtttaac tcccgaaccg gcaatattct
    18661 gcgtcagcaa atctcctttg cggaatccga cctaccactg atctaccgca cagagcgggc
    18721 attgcagctt atctgcgata agctccagca ggtggaactg aagcgcgttc cgcgcgtgga
    18781 gaatctgctc tattttatgc agaacacgcg taaacggctt gatgaaccgc cccggaaatc
    18841 ctg
//
LOCUS       DAZKIV010000088        18803 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZKIV010000088 DAZKIV010000000
VERSION     DAZKIV010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.088"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            271..837
                     /gene="yqaB"
                     /locus_tag="ACHOT6_002967"
     CDS             271..837
                     /gene="yqaB"
                     /locus_tag="ACHOT6_002967"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFP9948147.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            834..1262
                     /locus_tag="ACHOT6_002968"
     CDS             834..1262
                     /locus_tag="ACHOT6_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFP9948148.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            1335..2891
                     /gene="gshA"
                     /locus_tag="ACHOT6_002969"
     CDS             1335..2891
                     /gene="gshA"
                     /locus_tag="ACHOT6_002969"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFP9948149.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEIISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            3041..3556
                     /gene="luxS"
                     /locus_tag="ACHOT6_002970"
     CDS             3041..3556
                     /gene="luxS"
                     /locus_tag="ACHOT6_002970"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFP9948150.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="ACHOT6_002971"
     CDS             complement(3620..5158)
                     /gene="emrB"
                     /locus_tag="ACHOT6_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFP9948151.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="ACHOT6_002972"
     CDS             complement(5175..6347)
                     /gene="emrA"
                     /locus_tag="ACHOT6_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFP9948152.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="ACHOT6_002973"
     CDS             complement(6474..7004)
                     /gene="emrR"
                     /locus_tag="ACHOT6_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFP9948153.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="ACHOT6_002974"
     CDS             complement(7095..7430)
                     /gene="ygaH"
                     /locus_tag="ACHOT6_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFP9948154.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="ACHOT6_002975"
     CDS             complement(7420..8157)
                     /gene="ygaZ"
                     /locus_tag="ACHOT6_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFP9948155.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(8281..9462)
                     /locus_tag="ACHOT6_002976"
     CDS             complement(8281..9462)
                     /locus_tag="ACHOT6_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9948156.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDARN
                     RLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEAA
                     N"
     gene            complement(9754..10746)
                     /gene="proX"
                     /locus_tag="ACHOT6_002977"
     CDS             complement(9754..10746)
                     /gene="proX"
                     /locus_tag="ACHOT6_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFP9948157.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELPNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDSWIKAHQQQFDGWVNEALAAQK"
     gene            complement(10804..11868)
                     /gene="proW"
                     /locus_tag="ACHOT6_002978"
     CDS             complement(10804..11868)
                     /gene="proW"
                     /locus_tag="ACHOT6_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFP9948158.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(11861..13063)
                     /gene="proV"
                     /locus_tag="ACHOT6_002979"
     CDS             complement(11861..13063)
                     /gene="proV"
                     /locus_tag="ACHOT6_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFP9948159.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIELGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(13418..14377)
                     /gene="nrdF"
                     /locus_tag="ACHOT6_002980"
     CDS             complement(13418..14377)
                     /gene="nrdF"
                     /locus_tag="ACHOT6_002980"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9948160.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(14387..16531)
                     /gene="nrdE"
                     /locus_tag="ACHOT6_002981"
     CDS             complement(14387..16531)
                     /gene="nrdE"
                     /locus_tag="ACHOT6_002981"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9948161.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEILSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIANTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(16504..16914)
                     /gene="nrdI"
                     /locus_tag="ACHOT6_002982"
     CDS             complement(16504..16914)
                     /gene="nrdI"
                     /locus_tag="ACHOT6_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFP9948162.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            complement(16911..17156)
                     /gene="nrdH"
                     /locus_tag="ACHOT6_002983"
     CDS             complement(16911..17156)
                     /gene="nrdH"
                     /locus_tag="ACHOT6_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFP9948163.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(17404..17733)
                     /locus_tag="ACHOT6_002984"
     CDS             complement(17404..17733)
                     /locus_tag="ACHOT6_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFP9948164.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTATAVGIFIGA
                     LLSMRKS"
     gene            17885..18229
                     /locus_tag="ACHOT6_002985"
     CDS             17885..18229
                     /locus_tag="ACHOT6_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFP9948165.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(18266..18715)
                     /gene="alaE"
                     /locus_tag="ACHOT6_002986"
     CDS             complement(18266..18715)
                     /gene="alaE"
                     /locus_tag="ACHOT6_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFP9948166.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
BASE COUNT         4723 a         4770 c         4869 g         4441 t
ORIGIN      
        1 aaccttcagc aatgaaggta tcgaagagat agcggtatta accgcaggca ccagggagga
       61 taacgttgcg tcagcaacgg cccgaagggc gaacgccagt gagtaatcct cccggatgca
      121 ccatcttctc ccaaaaattt ccttcaaaaa tatctcattt ctaaataact actttatcca
      181 tttcctgaat tttcttctcc ttcgccatca gcgacaaaat caagcaatta cgctaaagta
      241 gcgtcatatt tttcgacttg cgagaagacc atgtacgagc gttatgcagg tttaattttt
      301 gatatggatg gcacaatcct ggatacggag cctacgcacc gtaaagcgtg gcgcgaagta
      361 ttagggcact acggtcttca gtacgatgtt caggcaatga ttgcgcttaa tggatcgccc
      421 acctggcgta ttgctcaggc aattattgag ctgaatcagg ccgatctcga cccgcatgcg
      481 ttagcgcgtg aaaaaacaga agcagtaaga agtatgctgc tggatagtgt cgagccgctt
      541 cctcttgttg aagtggtgaa aagttggcat ggtcgtcgcc caatggctgt aggaacgggg
      601 agtgaaagcg ccatcgctga ggcattgctg gcgcacctgg gattacgccg ttattttgac
      661 gccgtcgtcg ctgctgatca cgtcaaacac cataaacccg cgccagacac atttttgttg
      721 tgcgcgcagc gtatgggcgt gcaaccgacg cagtgtgtgg tctttgaaga tgccgatttc
      781 ggtattcagg cggcccgtgc agcaggcatg gacgccgtgg atgttcgctt gctgtgagtg
      841 aagcgttatc gcttttctcg ttgtttgcca gtagttttct cagcgctaca ctattacccg
      901 gcaactccga agtcgtgctg gtggcaatgt tgctttccgg gatcagttat ccctgggttt
      961 tagttttaac agcaacaatg ggtaatagcc ttggagggtt aactaacgtt atccttgggc
     1021 gtttctttcc attgcgtaaa acatcgcgct ggcaagagaa agctactggc tggctgaaac
     1081 gctatggtgc agtcacgcta ttattaagct ggatgcccgt ggttggcgat ttactgtgtc
     1141 tgttagcggg atggatgcgc atctcgtggg gaccggtaat cttttttttg tgccttggta
     1201 aagcgttacg ctatgttgca gttgcagcag ctaccgttca gggcatgatg tggtggcact
     1261 aattgtaggc ctgcacatat ggtcaccatt acagttatgc taattaaaac gattttgaca
     1321 ggcgggaggt caatttgatc ccggacgtat cacaggcgct ggcctggctg gaaaaacatc
     1381 ctcaggcgtt aaaggggata cagcgtgggc tggagcgcga aactttgcgt gttaatgctg
     1441 atggcacact ggcaacaaca ggtcatcctg aagcattagg ttccgcactg acgcacaaat
     1501 ggattactac cgattttgcg gaagcattgc tggaattcat tacaccagtg gatggtgata
     1561 ttgaacatat gttgaccttt atgcgcgatc tgcatcgtta tacggcgcgc aatatgggcg
     1621 atgagcggat gtggccgtta agtatgccat gctacatcgc agaaggtcag gacatcgaac
     1681 tggcacagta cggcacttct aacaccggac gctttaaaac gctgtatcgt gaagggctga
     1741 aaaatcgcta cggcgcgctg atgcaaacca tttccggcgt gcactacaat ttctctttgc
     1801 caatggcatt ctggcaagcg aagtgcggtg atatctcggg cgctgatgcc aaagagataa
     1861 tttctgcggg ctatttccgc gttatccgca attactatcg tttcggttgg gtcattcctt
     1921 atctgtttgg tgcatctccg gcgatttgtt cttctttcct gcaaggaaaa ccaacgtcgc
     1981 tgccgtttga gaaaaccgag tgcggtatgt attacctgcc gtatgcgacc tctcttcgtt
     2041 tgagcgatct cggctatacc aataaatcgc aaagcaatct tggtattacc ttcaacgatc
     2101 tttacgagta cgtagcgggc cttaaacagg caatcaaaac gccatcggaa gagtacgcga
     2161 agattggtat tgagaaagac ggtaagaggc tgcaaatcaa tagcaacgtg ctgcagattg
     2221 aaaacgaact gtatgcgccg attcgtccaa aacgcgttac ccgcagcggc gagtcacctt
     2281 ctgatgcgct gttacgtggc ggcattgaat atattgaagt gcgttcgctg gacatcaacc
     2341 cgttctcgcc gattggtgta gatgaacagc aggtgcgatt cctcgaccta tttatggtct
     2401 ggtgtgcgct ggctgatgca ccggaaatga gcagtagcga acttgcctgt acgcgcgtta
     2461 actggaaccg ggtgatcctt gaaggtcgca aaccgggcct gacgctgggt atcggctgcg
     2521 aaaccgcgca gttcccgtta ctgcaggtgg gtaaagatct gttccgcgat ctgaaacgcg
     2581 tcgcgcaaac gctggatagc attaacggcg gcgaagcgta tcagaaagtg tgtgatgaac
     2641 tggttgcctg cttcgataat cccgatctga ctttctctgc ccgtatctta aggtctatga
     2701 ttgatactgg tattggcgga acaggcaaag cgtttgcaga agcctaccgt aatctgctgc
     2761 gtgaagagcc gctggaaatt ctgcgcgaag aggattttgt agccgagcgc gaggcatctg
     2821 aacgccgtca gcaggaaatg gaaaccgctg ataccgaacc gtttgcggtg tggctggaaa
     2881 aacacgcctg acagaaaaga aaaaggccac tcgtgagtgg ccaaaatttc atctctgaat
     2941 tcagggatga tgataacaaa tgcgcgtctt tcatatactc agactcgcct gggaagaaag
     3001 agttcagaaa attttaaaaa aaattaccgg aggtggctaa atgccgttgt tagatagctt
     3061 cacagtcgat catacccgga tggaagcgcc tgcagttcgg gtggcgaaaa caatgaacac
     3121 cccgcatggc gacgcaatca ccgtgttcga tctgcgcttc tgcgtgccga acaaagaagt
     3181 gatgccagaa agagggatcc ataccctgga gcacctgttt gctggtttta tgcgtaacca
     3241 tcttaacggt aatggtgtag agattatcga tatctcgcca atgggctgcc gcaccggttt
     3301 ttatatgagt ctgattggta cgccagatga gcagcgtgtt gctgatgcct ggaaagcggc
     3361 aatggaagac gtgctgaaag tgcaggatca gaatcagatc ccggaactga atgtctacca
     3421 gtgtggcact taccagatgc actcgttgca ggaagcgcag gatattgcgc gtagcattct
     3481 ggaacgtgac gtacgcatca acagcaacga agaactggca ctgccgaaag agaagttgca
     3541 ggaactgcac atctagtcag taaactatct tcacaattaa ttgaaaaaag ccagttcaaa
     3601 tgaactggct tagttgtact tagtgcgcac cgcctccgcc gccacctgcg ccaaatggcg
     3661 gtttagcaaa ccacaccagc cccagcagta cgaggaatat cccggctgac atccagaaga
     3721 tctcattggc ggaaataatc agcccctgat tggtgatctg ctgggcaatc cagcctgatg
     3781 cctgctgttg cgtcatccca agcccttcca gttgactgta catcgcctgg gcattcgggt
     3841 tgaacgggtt taccgactca gtcaactgcg catggtgcat cgactcgcgg ttggtccaca
     3901 tggtcgtggt tatcgacgtg ccgatagacc ccgccagcgt tcgcgtaaag ttagagaggc
     3961 tcgatgccgc cgccagtcgt tccggtggca aaccagacag cgtaatggtg gtcagcggca
     4021 taaagaagca ggccaccgca aacccctgga taaactgcgg ccaggccgac gcgccaaaat
     4081 ccatacctgg ttcaaaggta taggcacgcc agtagaagca gacggcatac ataataaagc
     4141 tgaaggttac cagccgccgc atatccagtt tatgcgcgaa gcggccgata atcggcgaca
     4201 ggatcaccgg aataatccct accggcgcag aggccaaacc tgcccaggtc gccgtgtaac
     4261 cgtagacctc ctgcaacaac tgcggcagca gaacaatagc gccgaagtag agcatatacg
     4321 cgaggctgat acacaagcag ccgatggtga agttgcgcga cttaaacaac gacagatcga
     4381 ctatcgggtt atcgtcggtc agctcccaga caatcaggaa gcagatagcc accaccgcca
     4441 ccacggtaag gatgataatt tcctgtgatg aaaaccagtc cagctcttta ccgcggtcga
     4501 gcataatctg caggctgccg ataccaataa ccagcagtgc cagccccacg gcatcaatcc
     4561 gccgccgttc ggtgcgggtt tcacgtccgc gcagagtttg cagtgtcatc aacaccaccg
     4621 ccacgccaat cggcacgttg atgaagaata tccagcccca gtggtaatta tcgctgatat
     4681 aaccgccgag gatcgggccg caaattggcg cgacaatcac cgtcatcgac cacaacgcca
     4741 gcgcgatcga gcgtttggct ggcgggtagt tattcagcaa tagactttgc gaaagcggga
     4801 tcaacggccc ggcgacaatc ccctgaatca cgcggaagaa gatcagcata ttcaggctgc
     4861 tggagacacc acacgcccac gacgcaatag caaaggcgat ggtggaccaa aggaacagtt
     4921 tcacttcccc gacgcgcttt gccagccagc cggtaagcgg gatcgagatg gcattcgcca
     4981 ccccgaaaga agtgattacc cacgttccct ggctgagcga tgagcccaga ttcccggcga
     5041 tagtggggat cgccacgtta gcaatggtgg agtccagcac ctgcatgaat gtcgccagtg
     5101 acagcgcaat cgtcataatg accagttgcg cgccttccag cggtttttgc tgttgcatca
     5161 cacgcacctc tggattagcc agcgttagct tttacgatat cgtcgatcag tttattgaca
     5221 ggtgccaggc tgatttcacg cgcggtactt accgcgaccg gtgttgaacg tactttattt
     5281 gccagtacct gaccgtcacg gttagtggta ttgacgctca ccagcgtgga caaaccaatg
     5341 cgcagcgggt attgctccag ctgtttctgg tccagttcga tacgcacagg tagacgctga
     5401 acgactttga tccagttacc ggtcgcattt tgcgccggaa gcagtgagaa tgcgctacct
     5461 gtgcccatat ccagaccaac cactttaccg gtgtatttca catcatcgcc gtaaatatcc
     5521 gtggtgatag tgaccggctg accgatacgc atattggcaa tctgcgtctc tttaaagttg
     5581 gcatccaccc acatattggt ggctggaacg accgccatca gcggcgtcgt tgggctaatt
     5641 tgcgccccag gctgtaccgc gcggcgggag acataaccgg tcatcggact gacaatacgg
     5701 gtacgctcca gcgccagcca ggcgttacgt acttcggtgg cagcctgttg cacggcaggc
     5761 tgatcttcca gtttagtccc cagaatcatc gcctgattgg cattgtattg ttgaatagcg
     5821 acgtccagtt gcgcctgggc actggtgacg gcgtcgcggg cgtgttgcag ctcttcgcga
     5881 ccaatcaggt tggcattgcc cagcggcaca cggcggttgt agtcgctttg tgctttcgcg
     5941 agggcgattt tttgcacctc aatattcgcc tgcaactgtt tgctgttaat catcagctgg
     6001 tgggtttggc gaacgctgga agccagtgca gttttggctt tttcaaacgc ctggcgagca
     6061 tctgtcgggt cgagagtgac cagaacatcg ccttctttta caaaatcggt gttatcggcc
     6121 cagactttcg tcacgctgcc agacacctga gacataattt gaatttgatt ccctgccacg
     6181 tatgcgtcat cggtttcttc gaaatgacgc agtaccaaaa accaataaat ccctatcgct
     6241 acggcaataa ttataaagag caaggtgaga aggaggagca gacgcttacg tttgccgctc
     6301 ttctttaccg gttgctgcgg ggtttgagtc tccgcatttg cgctcatatt gttctccacg
     6361 atcttcttaa ctcatcggct gagccgacct gcttgccaaa aaggccagca tgttgcgatg
     6421 ctggccggtc attttttctt ttataaatct ggatttttga gcgagatgac gcgttagctc
     6481 atcgcttcga gaaccacacc gtcttgttcc atctggtcga gacgggagag caatttgcgg
     6541 gtgatttgct cgagctgatc tttttctgtt gtgctgagcg cggaccagag ttgatgcagg
     6601 cagttatgct gcggtggtaa aacctcgcgc aaaaactcgt gacctttttc cgttaattgc
     6661 agatgcaggc agcggcgatc gttatcgctt tcacgacgtt cgatccaacc gcgtttttcc
     6721 agttcatcgg caatacgcgt cgcgttggta cgggatgatc caagagcaca acttaattca
     6781 gaaggctgaa tactgtggtt ttcctgagac tccagcgtaa tcaacgccat aaacaacgtc
     6841 tcgttaatcc cctgagcctt cagcatttta ttgcggttct ccagcagctt gctttgcata
     6901 tgcatgcaaa gacgagtcag aaggatctcc tgataaggaa aatcttcgtg gcggctggcg
     6961 cgaaatttta gcatttgttc aatgggcgta aacgaactat ccatttgggt atgacctcat
     7021 taatttcagc cgatatagta acgacagtga caaataaagt aaatgtattg ttttagaaaa
     7081 atgattcttg tgggttatat aatcgccatc actttccagg cgagcccata ggccagcgca
     7141 ctaagcagtg ttgggatgat aatgctgcgt gttttataga aactggcacc cagtaccgcg
     7201 aagccgacca gcgtgggcac gaaacggcgt gtatcgtgca tcacttctgg tgcggtagag
     7261 acaaccagca gagcgcatat cgaggcgatg ccaatggtgt cgagcaaaat acctaccgcg
     7321 ccacgtttgg ttgggcgggc attacccaca cgcaggcgca gcggcaaata gcggaagcaa
     7381 taattcgcca cgccaaccag taacccaagc agcagaacct catagctcat cgggcgctcc
     7441 ttgccagaat gcctggatta acgcagtgag gcagccacag acaatgcctg ccagaatggc
     7501 gacgggaata gaaaatagcg ttacgcctgc aagggcacca actaacgctg cggtaacgca
     7561 aagagattgt ttgcgctgga aagaggcgag caggaaactc ataaagagtg ccggaagcat
     7621 aaaacctaat gcagcttcaa cggcgggata accttgcagc aagccgctgc cggagaatgc
     7681 ccctattacc gtaccaaata cccacgatga ccatgaactg aaggcaatgc cgatcatcca
     7741 gttctcgctc cagcggcgat tattgcgtac cagttttgcg gttgcggcgg caaaaacctc
     7801 atccgtcagg ccaaatgccc acagggcggt tttcgatttt tgcagacgct gaataatacg
     7861 gctacgcagt gacgggccat acaacacatg gcgaacatcc attgccatga cggtcagtgc
     7921 agcaacccac aaactactcc cggctgccag catcgcggta atgacgaact ggctcgcgcc
     7981 tgcataaatg atgcaggaga aaaaaacgct ttcgagagga gagaatccca gacgggtcgc
     8041 attcagaccg aacgcaaagg ccaccggaat ataactaata acaatcggta aactgtcttt
     8101 gcatccttcc atgaaggtcg ccgaaccagg agcaggctgt ggagtagggc tttccataga
     8161 gtgtacgctt aacgattgtt aatattataa atagactgaa tgaatatctt aaccttatca
     8221 gactgatggg cttcttaaca cccttataag tgtaaagcca cgaaaacggt tgctgattgc
     8281 ttaatttgcg gcttcctgac gatgaaaacc tcgccaccag accagtaagt taaccagggc
     8341 aatcgtcgcg ccagccagac aaacgccagc ccaaccgcca tgttgccagg ctgaggctga
     8401 aattagcgaa ccggcggcac cgccaataaa gtagctggtc atgtaacctg cggtcaggcg
     8461 attacgcgca tcagggtgta ttcgataaat taccgtctgg ttagtgatat gcacgccctg
     8521 tacggtgaga tccagcacca ggatgccgat aatcagcgcc agtacggaag tgtgaccaaa
     8581 ccagatcgcc agccatgaaa gtaatagcag cagaccgaaa gttgtggtgt ggtgcgattt
     8641 acccttatcg gcaaaaccgc ccgccggacg agcgcccaac gctccggcag ctcccgcaag
     8701 tccaaacaga ccaatgacac catcgctgta gttaaaaggt ggagcggcaa gcaaaaaggc
     8761 cattgaggtc cagagaatgc tgaagttggc aaaggtcagg cagcccagca atgcgcgggt
     8821 gcgcaggatc ttatcgctga taaacatgct gaaaacggaa cctaacaact gtgggtagtt
     8881 gaggtgggtt tctgatttca tttgtggcag accacgccat aatgccagcg ccatcagtgc
     8941 cattaacacc gaggcaaccc aaaagacggt gcgccagccg ccgagattcg ccagcaatcc
     9001 ggcaacggtc cgtgcgagta agatccccaa cagcagaccg ctcataatag tgccaaccac
     9061 tttgccgcgt ttgtccggtg aagccagcgt cgctgccagc ggaaccagaa tttgtgccac
     9121 gacagagaat aaaccggtta atgccgtacc gaggatcatc atcgccagcg actgactgct
     9181 ggcagtgatt aacatgccgc ctgccgccag taaggtcatc gagacaatca ggcggcggcg
     9241 ttcaaacata tcaccgaggg gaacaagaaa cagcagacct gctgcatagc ccaattgcgc
     9301 ggcggtaaca ataaagcctg ccgaactggc ggaaagggaa aagttacgcg cgatggtgtc
     9361 gagcaatggc tgagcgtaat agttactggc gaccgccaga ccggtggcga tagacatcag
     9421 cacgatcagc gccgggctaa gctcatgatt aggtttagtc attatttttg ttcatcaggt
     9481 gatgattaag ctggaaatca ataatagcgg atgttgatga atatgggatg aaagatagat
     9541 tgttgaaatg tgcgatgtgg tttgtaggca tgataagacg cgccagcgtc gcatcaggca
     9601 ttgtgcacag aacggcggat gcggcgaaaa cgccttatcc gccatacgga atgagtgtat
     9661 tttgtaggca tgataagacg cgtcagcgtc gcatcaggca ttgtgtatag agcggcggat
     9721 gaggcaaaaa cgccttatcc gcctgaataa aaattacttc tgcgctgcca gcgcctcatt
     9781 cacccagcca tcgaactgct gctggtgggc tttgatccag ctatcaacgt ggccctgaat
     9841 atcgccttct gaggctttgc cgtcatgcat aatggcgttc tgggcgttaa tatcggccac
     9901 tggcaactgc ataatggcaa acagtttggc tgccgccgga tttttctcgg tccaggcttt
     9961 gttggcaacg atatgcatgg tgctgaccgg gaagccgtaa ttcgcgccat tcggcagttt
    10021 ggtatcggca tttttatcgc ccggcagtgc ggagaacggc acctgcaacc agaccacatc
    10081 tttccctggc ttcagctcat tactcaccca gtacggcgtc caggtgtaat aaaacaccgg
    10141 tttgccctct ttgtagcgac tgatggtgtc ggccatcatc gctgcgtagt tcccctgatt
    10201 atgcgtcacg gtgttgggca gttcatacgc ggcaagctgg tggttgatcg caccttcgca
    10261 gccccagcca gggttacaac cggttaaatc cgctttaccg tcgccgttgg tatcgaacag
    10321 tttggcgatc ttcggatctt tcagttgtgc gatgttggtg attttgtact ggtcggcggt
    10381 tttcttatcg atcaggtaac cctgtgccgc gccgttaaca aatacccctt cacgataaaa
    10441 tttcttatcg ccaccggcag cttcgtacat gttgtcatgc agtggcgtcc agttcacggc
    10501 ggtgaaggtt gcatcgccgg aagcaagcga ggtgtagcca acgttgtaat ctacttcgct
    10561 gggtttgttg acggtatagc ctaatttctc cagcgcacgg ctgaccagca gcgtctggaa
    10621 ggtttcttcg gtgatggtgc tttgaactgg atttacagta atacctttgc ccggcagatc
    10681 ggcagcaaaa gtttgtgtag agataagcgt ggcaaacgct gtcgcaaaaa gtacgctatg
    10741 tcgcattgtt attccttttt ttggcagggt gataatgccc ggcgtcaccg ggcaagtgca
    10801 gagttactta atgaatgggc gggtcagcag accaacaggg ccagtggtgt accagcgacg
    10861 gttgccgcga ctgcgtgagt cgcgcccaac ggcctgcgtc aggcgatcga ggataatggc
    10921 gaggatcaca atcccgacgc cgccaacggt ggcaagcccc atatccagac gaccgatacc
    10981 gcgaagtacc atctgaccca gcccgccgac ggcaatcatc gaggcaatga ccaccataga
    11041 aagggccagc atcagcgtct ggttaacgcc cgccataatg gtcggcatcg ccagcggtaa
    11101 ctgaactttg aacagcatct ggcgcgggct ggcaccgaat gagcgcgagg cttcaatcag
    11161 atccgccgga acctggttaa tccccagaat ggtcagacgg ataatcggcg gcagagcaaa
    11221 gatgatcgtc accaccacgc ccggcacgtt accgatacca aacagcatga cgattggcac
    11281 cagataaaca aacgctggcg tggtctgcat ggcatcaagc agtggacgaa taattttcgc
    11341 cgctcgtgga cttctcgcca gccagatccc caacggcaaa ccgatgacga tacagaacag
    11401 cagggcggtt aacaccagcg ccagagtcac cattgcctgc gaccaggcac cgattgcgcc
    11461 gatggcaatc agcgaaacca gcgtcgccac acccattccg accccggaaa tctgccaggc
    11521 gatgagagcg aaaacgataa tcgccaccgg tgcgggcata cccagcagca attgctggaa
    11581 accgttgagg atataatcaa ccggaacgcg cacgccctgg aagacgggac ggaaatgggt
    11641 aacaacccag tcgatccctt cagtgaccca actgtcgagc gggatcagcg ttttatggaa
    11701 cggatcgaga atattaaaat gctcgacgtt tggcgcaggc gtactggtca gccagtcagc
    11761 accaccaccg tcagtcggtg cagtcgccgg tgtaccccag gcgtctgcgg attgcacggc
    11821 actgtccgcc gctggcgtgg tatcccacgg attattttga tcagccatta tttaccccct
    11881 cacgatctaa agcgcgcagc agcattcctt tcgaaatgat gccgacatac tgttggtcct
    11941 cgtcgaccac gggcaccgca cagggtgcct gtccgacatg agagagcaac tcgctaagag
    12001 gcgtttgtgc atcgactgct aacggcgcat caatcagcgc cgcatcaaga ccttgctgct
    12061 gcgttaacgc ggttttaagc gaatcgatgg aaactgcgcc gacaaactta ttaccgagtt
    12121 cgataacgta gccatattcg cgatcttcat cctgcaataa tttcagtgcc gaacgtgggc
    12181 cgaagccagg ggttttacga attaagccat tcggtgtccg gcgggcaata tctttcgcac
    12241 tgaatacctg actaatatca acgccacgga agaaggtacg gacataatca ttcgccggat
    12301 tattgagaat ttcatccggt gtgccgacct gtaccacttc accattttgc ataatggcaa
    12361 ttcggtcgcc aatacgcata gcctcatcga gatcgtggga aataaagaca atggtgcgct
    12421 gatgtttcgc ctgtaatttt accagctcat cctgcatctc ggtgcgaatt aatggatcga
    12481 gcgccgagaa ggcttcgtcc attaataata tatccggatt aatcgctaac gcgcgggcta
    12541 atcccacacg ttgacgcatc ccgccagaga gttcatccgg gtagctgtgg gcataatttt
    12601 ccagcccgac ctgacgcagt gcatcaaggg ctttttcccg gcgttcttcg gcattaattc
    12661 cggccaattc cataccgaac gcagtattgt ccagcacggt catatgcggc attaaggcaa
    12721 aggactggaa gaccatcgca atcttttttc tgcgcacctc acggagttcg gcatcggata
    12781 ttttggcaat atccacacca tcaatcagca cttgcccgcg ggtgggttca atcaggcgat
    12841 tgagaaggcg taccattgtg gatttacccg agccggataa tcccatgatg acaaatatct
    12901 cgccttcttc aatggccaga ctggcgtctt ttacgccaag cgatagccca gttttttcca
    12961 gaatttgttc tttcgaaagt ccttgttcga tatatttgaa cgctcgctgt ggatgctcgc
    13021 caaatatttt ataaagattt ttaatttcta atttaattgc catgcaatag aaagattcct
    13081 ttatttgttt atgtcgatat attacccaat ggaaatagtc acttttttct accctaacat
    13141 actgagaatc tgaggcaacc cctgagggca aatgtggcat gaatattgat gcgagcaaat
    13201 cgccggaatt tcccgtgata taagggctga gagcaaatcg agaaaagttg atattttttg
    13261 tcatagatag cgaaaaaacg cctgaatatt gtagtttcag gcgaataatg caacagagta
    13321 tcagtgtaga tcactacaga atatttgcgt gataaaaagc tatttggcgg gaattatttc
    13381 cctgctgcgg gtagtgatat ttttgaaaat aacaccctca gaaattccag tcttcatctt
    13441 ctgtttcaac cgctttcccc atcacataag aggagcctga accggaaaag aaatcgtgat
    13501 tttcatcggc attcggcgaa agcgcggcga ggattgccgg attcacttcc gccatttctg
    13561 cgggaaataa cggttcgtag cccagattca tcaaagcctt attggcgttg taacagagaa
    13621 acgctttcac atcgtcagcc cacggggttt cggcgtacag ctcatcggtg tattgcaact
    13681 cgttgtcgta gagttccagc aacaaatcga aggcgaaact cttcaactct tcacgttgtc
    13741 ccagagatat cttttccatg tttttctgat atttatagcc tatgtagtaa ccgtggactg
    13801 cttcatcgcg gataatcaga cggatcagat ctgcggtatt ggtcagcttt ccgcggctgg
    13861 aaaaatacat cggcagccag aaaccggaat agaacaaaaa agattcaaga aacacactgg
    13921 cgattttctt tttcagcgga tcatcaccgc gataatgttg ctgaataatc tgagcttttc
    13981 gctgcaacgg tgcgttttct tcactccagg cgtaggcggc atcgacatct ttggtctgac
    14041 acagcgtcga gaaaatcgaa ctgtaagagc gggcatgaac cgcttccata aagctgatat
    14101 tcgataatac cgcttcttca tgaggcgtga gtgcatcggg catcagcgaa ggcgcgccga
    14161 taacattttg cagcgtgtcg agcagcgtca ggccagtaaa aacgcgcatc gtcagttgtt
    14221 gttctacgac agttaatgtc tgccaggcag gaatatcgtt cgacagcggc accttttctg
    14281 gtagccagaa attgctggtc aggcgattcc acacctccag atctttatcg tcagatatct
    14341 tgttccagtt gatggcgctg atacgtgaga gtttcataga tattccttaa agtgcacagg
    14401 agacgcagcc ttcaatttca gtgccttcca gcgccatctg acgcaggcga atgtaataga
    14461 gcgttttgat acctttgcgc caggcgtaaa tctgcgcttt gttgatatcg cgagtggtgg
    14521 cggtatcggg gaaaaacagc gtcagcgaca aaccctgatc gacatggcga gtcgcttccg
    14581 cgtaggtgtc gatgatcttt tctgcgccga tttcgtaagc atcctgatac agcgccagat
    14641 tctcgttagt cataaacggg gcagggtagt aaacgcgtcc tgttttgccc tctttgcgta
    14701 tctctacttt cgccacaatc ggatgaatac tcgacgtagc atggttgata taagagatag
    14761 aaccggttgg cggcaccgcc tgaagattct ggttataaat gccgtagcgc atcacgtcgt
    14821 cgcgcagttg cgcccacatc tcacgggtag gtaacgtaat accgctacgg gcaaacagtt
    14881 cgccaacttt cgccgttttc ggctgccagt tcccttgcag atattggctg aaatattcac
    14941 cactggcata gcgtgactgt ttgaacccgg cgaaggtttc accgcgttcg cgtgccagca
    15001 acatcgaggt acgcagtgcg tgccaggtga tggtatagaa atagagattg gtgaaatcca
    15061 gtgcttccgg cgaaccataa gcgatgcctt ctcgcgccag ataaccgtgt aaattcatct
    15121 gccccagccc gatggcgtgt gaggcggcat ttccggcttc gatggacggc acgctgcgga
    15181 tatgactcat atctgacacc gccgttaaac cgcgtacggc agtctctacc gtgcgggcaa
    15241 agttggggga atccatggtg ttggcaatat tcaacgaacc taaattgcag gaaatatcat
    15301 ggcctgtacg ggcatagtcg agattctcgt catactctga ggcgctgtta acctgcaaaa
    15361 tttctgagca gagattactc atatttatgc gcccggcgat agggttagca cggtttaccg
    15421 tgtcttcata catgatgtag ggatagccgg actcaaactg gatttctgcc agtcgctgga
    15481 agaaatcacg ggcgttgagg tattttttgc gaatgcgttc atcggcaacc agttcgtcat
    15541 agtgttggct gatggccaca tcggcaaacg gcttgccata aactcgctca acgtcatagg
    15601 gcgaaaacag cgccatctgc gcattctctt ttgccagatg gaaagtgata tccgggatca
    15661 ccacgcccag cgacagtgtt ttaatgcgga ttttctcgtc ggcattttcc cgtttcgtat
    15721 cgagaaaacg cagaatatcg ggatgatggg catgtaaata gactgcacca gccccctgac
    15781 gagcgccgag ctggttggca taggaaaatg cgtcttccag cattttcatc accggaatca
    15841 cgccagaaga ttgattttca atacgtttaa ttggcgcgcc cgcttcccgc agattcgaca
    15901 gcaaaaatgc cacgccgccg ccgcgtttcg acagttgcag tgcggaattt accgcccggc
    15961 caatcgactc catattgtct tcaatacgca gcaaaaaaca ggaaaccagt tcgccgcgct
    16021 gctgcttacc acagttgagg aatgtcggtg ttgcaggctg aaagcgtcct gacagtatct
    16081 catcggtcag ttgcaacgcc agtgtttcat cgccttgtgc cagcgttagc gccaccatcg
    16141 tgactcgatc ggcaaaatct tccagataac gcttaccgtc gaatgtcttc aacgtatagc
    16201 tggtgtaaaa cttccatgcc ccgaggaatg tatggaaacg aaaaccgctg gtgtgtgcgt
    16261 gggcaaacag cgtaatgaca aagtcgcgag agtagcgatt aagaacgctt tcatcatagt
    16321 aaccttcgtt gaccagccag ttcaggcgct gctgctggct actgaaggtc acactgttcg
    16381 gacgcacatg cgtcgcaata aaggcgtcaa cggcctggcg gtctttatcg aactgaatgc
    16441 gacctgcgct atcgtagagg ttaagcatcg cattcagcgc gtggtaatcc atcgtttcct
    16501 gcgtcaggca ttctgcggtt gtcgttgcca aaattcggtt actcctttac gaacgttttc
    16561 gatatcgctt tgcgtaccca tgagttcaaa acggtacagc cacggcacgc cgcatttctg
    16621 agcaatcaca tctccggcac ggccatacgc ctcaccaaag ttgcgattac ccgaagcaat
    16681 aacgccgcga agcaacgccc ggttgtgctc gtcgtttaaa aagcgaatta cctgtcgtgg
    16741 caccgcgcca gccgtaccgc cgccgccgta agagggcacg atcaggatgt aaggctcgtc
    16801 tacctgaatc cgttcccgct cattgagcgg gatgcgcacc gcgggcagtc ctaaacgttc
    16861 gataaaacgc tgcgtatttt cggagctact ggaaaagtag acgagctggc tcatgcactg
    16921 gccgcgtgtg gcgctggatg cagacgattg atcatgtccg ggcggaaacc agaccagcta
    16981 agatcgccag caatcactac cggcaactga cgaaagccct gagcacgcag cgcttccgcc
    17041 gcttcaggaa cgcgatcgac attaatcatt tcaaaatcaa agccccggtt ttccatcgcc
    17101 cgtttggtgg cgtggcactg aacgcaatcg ttacgagtgt aaatagtaat gcgcatgatt
    17161 cgtatttccg tttaaaatga agatacggcg cgatgatacg cgtcgggttg tctctctgtt
    17221 gatacagaga tactagatgt agttgaaaaa agattcaacc acacaatata tagcaaatag
    17281 tggtcgaaat taccctggat atgagcgtga cggggtaggg ggatttttgt gattcaccag
    17341 gcaaaaagaa accccgaaga caggcttcgg ggtcaaagac gcgtatttat tatcattttt
    17401 gcactacgat ttgcgcatgc ttaacagtgc gccgataaaa atacctaccg cagttgctgt
    17461 acccacgcta caccagggtc tttcacgaac aaaagaatcc gcgcagccaa cggcatcgcg
    17521 tgcggcttgc tggacgcgag tacgaccatg cattcgcgcc ctggtttctt tcagcaatgc
    17581 ctgagcttta ctgcgtgcgg cttcagcttc ccctttggcg tcgctgcccc aggactttaa
    17641 tacagattcg aggctgtcag ctaattgatt gacatcattc tggatatcct gcacgccatc
    17701 atcaacgtcg ttgcggttcg gtcggttaaa catatgatcc cccattagaa ttcgatgtaa
    17761 ttttcagtgt agaccataat tttcttaact gaggttcatc gcggctttat ggacttttct
    17821 gaaagcgttg tgaatgcgca atcaggtaag gtaggcccgc ggttgataat aacgaggatc
    17881 aaatatgtat ttacgaccag acgaggtggc gcgcgtactt gaaaaagtcg gttttactgt
    17941 cgatgtggta acccagaaag cgtatggtta ccgccgtggg gaaaattatg tctatgttaa
    18001 tcgcgaagcg cgaatgggac gtaccgctct ggtaattcat ccaacattaa aagaacgcag
    18061 ttcaacactg gctgaacccg cttccgatat taaaacttgc gatcattacc agcaatttcc
    18121 gctctattta gcaggcgagc gacacgagca ttacggtatc ccgcatggct tcagttcgcg
    18181 tgttgcgctt gaacgttatt tgaatggttt atttggcgaa gccagttaaa gacgcgactg
    18241 gcgatgccag tcgcgaaaag aagagtcagg cttttacctg ctggtaacgg ctgactttaa
    18301 acagtcggcg gcaataatcg aggaagtagc cataaaccgc ccccatcaac atcgaaacaa
    18361 cgatgtttga actgaccgcc gccataatct gatgccagtc tgcgcccacc actaacaaga
    18421 tcgccacata caccggtgac tggaacgtca cataagccag gatatcagcc agatttttta
    18481 tccagcccga cgggctaact ttgcgtgccg cgcgcataaa cagatcgcgg tacataccgt
    18541 atggccatgc aattaagatg ttcaccggaa tcgctaccaa tctggaataa aaagactgtt
    18601 cgaagctcat tccggagagg aaaacttcaa tacacatgtt cacgacagaa cagtaaacaa
    18661 ccatcgcgaa cgtatctgca actgcatgac gcaagcgtga ctgcggtgag aacatgctaa
    18721 tgctccttaa taaaagcgta atgggatgtt aatggagatg aaagcagtgg atctcattgc
    18781 catctgcatt ttcgcatagc gta
//
LOCUS       DAZKIV010000089        18611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZKIV010000089 DAZKIV010000000
VERSION     DAZKIV010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.089"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            125..1345
                     /locus_tag="ACHOT6_002987"
     CDS             125..1345
                     /locus_tag="ACHOT6_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9948167.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQNLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1318..1947
                     /locus_tag="ACHOT6_002988"
     CDS             1318..1947
                     /locus_tag="ACHOT6_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFP9948168.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSSFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1948..3108)
                     /gene="ybdL"
                     /locus_tag="ACHOT6_002989"
     CDS             complement(1948..3108)
                     /gene="ybdL"
                     /locus_tag="ACHOT6_002989"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFP9948169.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3216..4304
                     /gene="hcxA"
                     /locus_tag="ACHOT6_002990"
     CDS             3216..4304
                     /gene="hcxA"
                     /locus_tag="ACHOT6_002990"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFP9948170.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWICGER
                     AIAAAQTKLPPAFELPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4314..4511)
                     /locus_tag="ACHOT6_002991"
     CDS             complement(4314..4511)
                     /locus_tag="ACHOT6_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFP9948171.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4693..6798)
                     /gene="cstA"
                     /locus_tag="ACHOT6_002992"
     CDS             complement(4693..6798)
                     /gene="cstA"
                     /locus_tag="ACHOT6_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFP9948172.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6979..7392)
                     /gene="entH"
                     /locus_tag="ACHOT6_002993"
     CDS             complement(6979..7392)
                     /gene="entH"
                     /locus_tag="ACHOT6_002993"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFP9948173.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7395..8141)
                     /gene="entA"
                     /locus_tag="ACHOT6_002994"
     CDS             complement(7395..8141)
                     /gene="entA"
                     /locus_tag="ACHOT6_002994"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFP9948174.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAEVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8141..8998)
                     /gene="entB"
                     /locus_tag="ACHOT6_002995"
     CDS             complement(8141..8998)
                     /gene="entB"
                     /locus_tag="ACHOT6_002995"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFP9948175.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9012..10622)
                     /gene="entE"
                     /locus_tag="ACHOT6_002996"
     CDS             complement(9012..10622)
                     /gene="entE"
                     /locus_tag="ACHOT6_002996"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFP9948176.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLTGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLAVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10632..11807)
                     /gene="entC"
                     /locus_tag="ACHOT6_002997"
     CDS             complement(10632..11807)
                     /gene="entC"
                     /locus_tag="ACHOT6_002997"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFP9948177.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12182..13138
                     /gene="fepB"
                     /locus_tag="ACHOT6_002998"
     CDS             12182..13138
                     /gene="fepB"
                     /locus_tag="ACHOT6_002998"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFP9948178.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSATQVLERLKALF"
     gene            complement(13142..14392)
                     /gene="entS"
                     /locus_tag="ACHOT6_002999"
     CDS             complement(13142..14392)
                     /gene="entS"
                     /locus_tag="ACHOT6_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFP9948179.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14503..15507
                     /gene="fepD"
                     /locus_tag="ACHOT6_003000"
     CDS             14503..15507
                     /gene="fepD"
                     /locus_tag="ACHOT6_003000"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFP9948180.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15504..16496
                     /gene="fepG"
                     /locus_tag="ACHOT6_003001"
     CDS             15504..16496
                     /gene="fepG"
                     /locus_tag="ACHOT6_003001"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFP9948181.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHISRRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16493..17308
                     /gene="fepC"
                     /locus_tag="ACHOT6_003002"
     CDS             16493..17308
                     /gene="fepC"
                     /locus_tag="ACHOT6_003002"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9948182.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17305..18438)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOT6_003003"
     CDS             complement(17305..18438)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHOT6_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFP9948183.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4389 a         5262 c         4793 g         4167 t
ORIGIN      
        1 ataactttca tctatttatt cacagcgcga atgaaaaata aaacactctg ctttgcagtg
       61 tttactttat aattaacaga cttatctcac tgatcaccct gtaacgttca gagagcgtct
      121 tcagatgtct atttataaaa ttcctcttcc gctcaatatt ctggaagctg cacgagagcg
      181 tattacctgg acactcaaca ccctaccccg cgtttgtgtc tcattttctg gcggcaaaga
      241 ctctggttta atgctgcatc tgacagccga acttgcccga caaatgggca aaaaaatctg
      301 cgttttgttt atcgactggg aggcgcaatt ctcttgcacc attaactatg ttcagtccct
      361 gcgcgagttg tacaccgatg tcatcgaaga gttttactgg gttgcgctcc cgcttacgac
      421 gcaaaattcc ctttcacaat accaacccga atggcagtgc tgggaacctg atgtcgaatg
      481 ggtgcgtcag cccccgcaag atgcgataac cgatcctgac tttttctgct tttaccagcc
      541 aggcatgacc ttcgaacaat ttgtacgtga gtttgccgaa tggttttcac aaaaacgtcc
      601 ggcggcgatg atgatcggca tccgtgcgga tgagtcctac aaccgttttg tcgccatcgc
      661 cagtttaaat aaacaacgtt ttgccgacga taaaccatgg actacggccg caccaggcgg
      721 tcatagctgg tacatttacc ccatttacga ctggaaagtg gctgatatct ggacctggta
      781 tgcaaatcat caaaatctct gcaacccact gtataaccta atgtatcagg caggcgttcc
      841 tctgcgtcat atgcgaattt gcgaaccttt tggcccggag caacgacaag gattatggct
      901 ctatcacgtt atcgaaccgg atcgctgggc tgctatgtgc gcacgagtca gcggggtaaa
      961 aagtggcggc atttacgccg gacatgataa tcatttctat gggcatcgga aaatcctcaa
     1021 gccagaacat ttagactggc aagaatatgc attattgctg ctcaatagca tgccggaaaa
     1081 aacagctgag cattaccgca ataaaatcgc catttatttg cactggtatc agaaaaaagg
     1141 catcgaggtt ccacaaaccc agcaagggga cattggcgcg aaagatatcc cctcctggcg
     1201 gcggatatgc aaagttttac tcaataacga ttactggtgt cgggcattat catttagtcc
     1261 tacgaaagcg aagaactatc agcgttataa cgaacggata aaaggaaaac gtcaggaatg
     1321 ggggatacta tgcaacaacg attaacgcag gatttaacgc aatttctcgc cagcttgccg
     1381 gaagatgatc gcatcaaagc gattaatgaa atccgcatgg cgatccatca ggttagctcc
     1441 tttcgtgaag agccagtgga ttgcgttctg tgggttaaaa acagccaact tatgcctaat
     1501 gattacaacc caaacaacgt ggcaccaccc gaaaaaaaac tgctgaaaaa atccattgaa
     1561 atagatggtt ttacccaacc cattgtggtc acgcatacgg ataaaaatgc cctggaaatc
     1621 gtggatggtt ttcaccgcca tgaaattggt aaaggctcat catcattaaa attacgctta
     1681 aaaggttatt tgccggtgac ctgtctggaa ggtacgcgta atcaacgcat cgccgcgact
     1741 attcgtcata atcgcgctcg tggacgccat caaatcaccg ccatgtcgga aatcgtccgt
     1801 gaacttagcc agttgggatg ggacgataat aaaattggca aagagctggg aatggacagc
     1861 gacgaagtgc tgcgcctgaa gcaaattaac ggcctgcaag agctgtttgc cgaccgtcaa
     1921 tattcccgcg cctggacggt taaataacta aagctggcgc aggcgttcag ctgctgccag
     1981 caacgtcgat tccttcttgg caaaacagag acgaatcagt ttatgtggga agggatcggc
     2041 gcaaaacacc gacagcggaa tcgccgctac gccgtgctcc tgcgtcagcc actggcaaaa
     2101 ctcaacatca tccagggtag atacggcgct gtaatccacc agcaaaaagt atgtaccttc
     2161 acacggtaaa atctccagcc ggctttcatt taaagcattc accagaatat cgcgcttctg
     2221 gcgataaaag tccggtaacg caagataatg ctcaggttct gcacgtagca tatcagcaag
     2281 cgccagctgt gccggggtat tcaccgaaaa ggtcagatac tgatgtacct tgcgaatttc
     2341 ggcgctgatg ggcgctggcg caacacaata acccactttc cagccggtca tatgataggt
     2401 cttgccaaat gaagaaaccg ccactgcccg ctcacgcagc tgcggatgcg ccagcacact
     2461 ggcatggccc tgttgtgaaa agttgatgtg ctcgtagact tcatcgctaa tgacaaaaat
     2521 ctcgtgcccg gcgatcgcct gccacaaagc ggcgaaatca gcctgctgcc agacagttgc
     2581 actgggatta tgcggagtgt tgaggatcac cagtctggtg cgctcgctta acaatgcggc
     2641 aaattcctgc cagtcaacgc gaaaatgcgg tggttgcagt gccatacgct tcactattcc
     2701 cccagaaagc gcgatggcgg gggcgtaact gtcatagctg ggatcaaaac aaatcacttc
     2761 atcgccattg cgcaccagtg cggtaatcgc cgcgtataac gcttccgtcg cccctgccgt
     2821 tacggtgata tcgctatcgg catctggttg atagccatac aaacgttccg ttttctgagc
     2881 aatcgcctcg cgcaaggcct gcacgccggt catgggcgcg tattggtttg ccccctgggc
     2941 aacgtggtac gccagccgct cctgtaaata gcgcggacca tcaaaatcag gaaagccttg
     3001 cgacaggtta atcgcctggt gttgctgcgc cagcgcgctc atctgggtga aaatagtggt
     3061 gccaagttgt ggaagtttgc tttgtggaat cagagggtta tttgtcattt tttattgtgc
     3121 ctgacgaagt tgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt
     3181 tagatgtcta aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg
     3241 tcgtcggccc ggctaactac ttttcacatc caggaagttt caatcacctg cacgattttt
     3301 tcactgatga acaactttct cgcgcggtgt ggatctgcgg cgaacgcgcc attgctgcgg
     3361 cgcaaaccaa acttccgcca gcgtttgaac tgccaggggc aaagcatatt ttgtttcgtg
     3421 gtcattgcag cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg
     3481 tggtgattgg cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc
     3541 tcggtctgcc atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc
     3601 tctccgtctg gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca
     3661 attttatggt gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg
     3721 cggggatcgg tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag
     3781 aaacgttgcc gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat
     3841 tgttaaacag tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat
     3901 tttgcgatgt ggtggatgct attattgctg gtggtgggat ggtcggtggt ctgggcgatc
     3961 gttttacgcg tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa
     4021 ccgagaaatt tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc
     4081 tgggtcagga tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga
     4141 ccacactggc ggagctggaa gtggatatca ataatcatgc ggagatcgac aaagtgatta
     4201 cccacaccct gcgtccggtg gaatccattc attacctgcc agtcacgctg acaccagata
     4261 cgttgcgtgc agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag
     4321 cagcgcgcgc cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg
     4381 taggtcatcg gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa
     4441 tcaggcatac caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt
     4501 gaatcaaaca tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga
     4561 acgccttatc cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg
     4621 ggagagggtt agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc
     4681 ctcactctga ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg
     4741 cattggctca tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc
     4801 cgtcttaatc gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc
     4861 ggcatccaga cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg
     4921 aatattgccg ctgtcgatca ttgcctggaa cttattagca atggccagga agccgacttt
     4981 cgcatccggg ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc
     5041 cgttggtacc agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc
     5101 acagagcatc agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag
     5161 agtgttaatg ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag
     5221 tacgcacagc gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg
     5281 agacaccacg cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac
     5341 cgccgtcaga ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc
     5401 catcatacca cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc
     5461 accgcccgca cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt
     5521 atctggtgta atgctaaagc cccagctact caccacctgc gcggcagaag cgactacatc
     5581 cgccgtccct gccggagcca gcaccgccat tgggctgttc atggcaaaat acacgcccgg
     5641 atcgatgata caggcagaaa ccagcgccat aatcgccacg aaggattcca tcaacatccc
     5701 accgtagccg ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga
     5761 agagatcagc gcatggaagc cagacaccgc accacaggcg atggtgataa acaggaacgg
     5821 gaacaggtta ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat
     5881 ggtcagcgtc gggcgcataa tcaaaatgcc taccgccaga ccgacgatag tcccgatttt
     5941 caggaaggtc gagagatagt cacgcggggc cagcagcaac cacaccggca gcaccgccgc
     6001 cacaaaaccg tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg
     6061 tgcccaggtc ggactttctg ccacccagcc gccagagata atggcgaaaa tcaggaacac
     6121 caggccaatc actgacactt cgccaatgcg gcccggacgc agatagcgca ggtagattcc
     6181 cataaaaatc gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt
     6241 cagggctttc accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca
     6301 ggctaccagc gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag
     6361 cgatcgaccg tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccagc
     6421 gagaaccacc ccggcgagca gccagatcat ccctggcagg taacccattt gcgccgccag
     6481 taccggccca accaacggac ctgctccggc aatggccgca aaatggtgac cgaacagcac
     6541 tttcttgtcc gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg
     6601 cgtcgggtca accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat
     6661 cagatagata cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt
     6721 taaagcaatg tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta
     6781 tttccctgat ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg
     6841 tttctataac atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac
     6901 caatatttaa cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct
     6961 catttaattc tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg
     7021 ccgcccctgt tcatcgaaaa cgacgatttc ccagctctga ttttgccgcc caagatgcag
     7081 cggctggcag acgccgcgta ccttgccttc agacaccggg cgatgatggg tggcgttaag
     7141 ttccgtaccc accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag
     7201 cgtttcagcc agcgccgctg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg
     7261 ggtatcaacc ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc
     7321 cagatgcgcc accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg
     7381 tttccagatc atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg
     7441 gtgatatggc tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga
     7501 cgggcgattt tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc
     7561 tgttcttcgg cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca
     7621 ggcgaaacca cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc
     7681 aggcttttca gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt
     7741 gcggcgtcgg acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg
     7801 gtttgctgaa acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc
     7861 tctttactga gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg
     7921 tccagtcgct ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg
     7981 gcaacatcca tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa
     8041 ccggtaactt ccgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta
     8101 cctgcgccgg ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga
     8161 gtagcttcca ccatgcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc
     8221 catgcacttt gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt
     8281 cgatcaggtt gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggatcacct
     8341 cacgcagcgc cgctttgctg gcggggatag gtgctggcag caattcttcg gtcatcacca
     8401 cccggccaga acgtccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat
     8461 cggccagcgc atctgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg
     8521 tggtcataca accaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct
     8581 ctttgagcat ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca
     8641 ccgtgtcgtc ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg
     8701 tcaggcctgg cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg
     8761 gctgggcggt gtaataaacc gggatattgt gctgtttgca gtagtcgcgt agtgccgcaa
     8821 tattcgcgat cacttgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt
     8881 cctgcatatc atggattaac aacgcggcac gttgcggttc aaaggcccag tcgactttat
     8941 tctgcggaat atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg
     9001 tgttctctcc ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg
     9061 actttcccga ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta
     9121 aattcggcaa taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct
     9181 tttaccgcca gataagcgca gcttttttcg cccatcagct catcttccat gctcaccagt
     9241 gcggcataga tcactgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc
     9301 ttctcgccgc cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct
     9361 ggatcaatag agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca
     9421 ttgtgctgcg gacttttgta atagccacgg aaggtgtacg gcccgcgcgt catcaggcgt
     9481 ccaacttccc cttgcggcag tggatttcct tcggcatcgg caacccacac ttcatcgtcc
     9541 ggacacattg ggtaaccctg ggtatggata attttctccg cgctatcatc aagacgggtg
     9601 tagttcacca gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg
     9661 ggaatacgcg ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc
     9721 gaggcaagct gcgcccggct ttcaccttcg gtcagcgcct gcaaccacag gctgactgcg
     9781 ggtggcacca gcgcggtgac gttcacctga tgtttttcaa tcaatgggaa gcaaagcgtg
     9841 gcgctgggat cggccgccag aacaaccgtt cctccggtaa gaaagacgcc cagcgatccc
     9901 ggcgaactca tggcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt
     9961 gtgaactgac aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc
    10021 gggatcagtt tcggtgtgcc ggtggtgccg ccggaaagct ggaaataggc cacttcatca
    10081 gcaggtgatg gcgtggcagt aaaatcctca gccggatggt taatcgcatc ctgcaagtta
    10141 tgctcaccgc tgtcgttgtg cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa
    10201 gtattgagga aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc
    10261 ggttcaatct ggctggcata ggcgttcagt tcactacgct gatggctgaa cagcgccagc
    10321 accggcgcaa cgcccagttt cagcagcgcg aaaaaggtaa tatacaattc agcgacgtta
    10381 cccagttgta ccagcgcggt ttcaccaggt ttaatgccct gacggcgtaa actacacgcg
    10441 aggttatccg ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc
    10501 gcgatgctgt cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag
    10561 tagccttttt cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc
    10621 atcctcgctc cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt
    10681 tcgcgccact cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc
    10741 acctgatttt cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg
    10801 ctgtcacacc aacccacaat gccgccaaac agttcgcggt cgaatggctc cagttcagca
    10861 ataacctggg tcgcggcctg atgcgggaaa ccgctcagcg cgggggttgg atgcagcaga
    10921 caggccagag tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga
    10981 tgccacagcg tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg
    11041 cgttcgcgca gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt
    11101 tctgacgcca gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc
    11161 gcggaaccgg ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc
    11221 gggctggccc ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc
    11281 gggttttgcg caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg
    11341 atatcaatca atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg
    11401 gcgcgggcaa ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg
    11461 ttcagcgact ggctgcgggt aaaacggcgg gctgaggtct gtttttcctg gcgggagaac
    11521 gactgccagg attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc
    11581 ccgaccatca ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt
    11641 ttttgctgga agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg
    11701 aaacatcctg acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca
    11761 agtgttgcca tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccata
    11821 aaatgataaa gactttaata atgattatca tttatatttt cggtcgctaa cctaaaagca
    11881 aacgcgcact atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt
    11941 gtggttacaa tgaaaatgag aagcattatt gatggattcg cataagcgca atgtgatgtc
    12001 ctgcgccgtt ctgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg
    12061 caccagtgct ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat
    12121 tgtgcgcttt gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgatt
    12181 tgtgagactc gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg
    12241 aatagccgca gttcaggccg ctgactggcc gcgtcagatt actgacagcc gtggcacaca
    12301 tacactggaa agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggctcact
    12361 gctggcgatt gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc
    12421 ggatgaccag ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg
    12481 gctctatatc ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt
    12541 aattagcgca accggcgggg attcggcgct ggcactgtat gatcagcttt ccaccatcgc
    12601 gccgacatta atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg
    12661 cgaaattacc gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact
    12721 ggcagcggcg aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac
    12781 cgccgctgca cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga
    12841 acaactcggc tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg
    12901 caaacgccat gacatcattc agcttggtgg ggaaaatctg gctgcggggt taaacggtga
    12961 atcgctattc ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact
    13021 gctcgcgcac ctgcccgcag tacaaaacaa gcaggtttat gcgctgggaa ccgagacgtt
    13081 ccgtctggat tactacagcg ccacgcaagt gctggaacga cttaaggcgc tgttttaagc
    13141 attaactgtc agacgctgtc acctgcggcg gcgtctggcg aaaacgtcgc aactccacca
    13201 gcaccagcaa taacaacacg ccgataatca acaaaccaaa accgctcgcg cttgcggaag
    13261 caaccggtgt catcatcgcg cccaaaccac ccagcagcgc cgcgcctatg gcatcgcccg
    13321 tcacgttctg cgccgtccac aaaccgttaa tccgccctaa catcgcttcc ggggtttgcg
    13381 tttgcagcat tgtatattgc agcaacgaac taaccgcgct cagccagccg aacagcgcca
    13441 gacaaaccac gcctaaaatc cacatcggca tcaggccaaa cagaccaatg gcgaggaacg
    13501 atcccagcgt ggagagcagc atcaataacc ctggtcgcgc actatgtgcc agcttcccgc
    13561 tggttaacgc accaatagcc gcgccgagcg ggatcgccgc gtagagaaaa ccaatctgtg
    13621 ccgctgacat ctgccagttg tcagccagcg ccggatacag tacccgcacc gcgctcgcca
    13681 tcgtcaataa accacccagc aacgcaatcc ctcctaccag cgggctggcg agcagaaaac
    13741 gaaatcctgc cagtaatgat ttcaacggat gctcgcgcgg ctgcggtggc ggtggcaacg
    13801 ccggaaggct taacaacggt agcaaggtaa taaacgtgcc cgccgccgcc agcccgtagt
    13861 tccaggctac gccaccggtc gccagcaata aaccgccaat catgggcgaa atcaccgacc
    13921 ccagacgcac ggtcaacatg gtgatcgccc cagcctgcat taagttttca cgccctacca
    13981 gtgctggtgt cgccgccaat agcgccgtaa cgccaagcga tgcgaaaaaa ccatcccata
    14041 aaccaagtaa atagattgcc agcaatgacg gctccggcag cagtgcatta aggcacagtc
    14101 caatgaagcc aatgccacag gtgccgcgcg ccagcaaaat cacttttttg cgctcatagc
    14161 gatccgccag cacaccgcca accatcaggc caacaaacat cgcgccgccg gtcagcgtca
    14221 ccgaaagccc cacctgccag gtagaatgcg tcatcatctg gatctgcacc ggcaccgcga
    14281 cgccgagcaa acccagagac acaattgaga taaaacgagc gaggaatact gcgcgaaacg
    14341 ccgggtgcgt tttcaacagg ctgaggttaa gcagccagga ttgtttattc attacaatgc
    14401 cttgccatca aatattttaa atacctattc ttaggctgca catgctaaca tatccaaata
    14461 agatcgataa cgataattaa tttcattatc atggaagttc gtatgtctgg ttctgttgcc
    14521 gtgacacgcg ccattgccgt gcccggattg ctgttattac tgattatcgc gacggcatta
    14581 agcctgctca ttggggcaaa atcactcccc gcttccgtag tgctggaggc cctctccggc
    14641 acctgccaga gcgccgactg caccatcgtg ctcgacgcgc gactgccgcg cacccttgcc
    14701 ggtttacttg caggcggcgc gcttggcctt gccggggcgt tgatgcaaac cctcacccga
    14761 aatccacttg ccgaccccgg cttgcttggc gtgaatgccg gagccagctt tgccattgtg
    14821 ctgggtgcgg cgctgtttgg ttactcatcc gcgcaggaac aactggcgat ggccttcgcc
    14881 ggggcgctgg tggcctcatt gattgttgcc tttaccggca gtcagggcgg cgggcagtta
    14941 agtccggtgc gtttaaccct ggcgggcgtg gcgctggcgg cggtgctgga aggactgacc
    15001 agcggcatcg ccctgcttaa tcctgacgtc tacgatcaac tgcgtttctg gcaagccggt
    15061 tcgctggata ttcgcaatct acatacctta aaagtggtgc tgatcccggt tctgatcgcc
    15121 ggagcaactg cgctattact gagtcgcgcg ctgaacagtt tgagcctcgg cagtgacacc
    15181 gcgacggcgc tgggcagtcg cgtggcgcgc acacagttga ttggtctgct ggcgattacc
    15241 gtgctttgtg gtagtgcgac ggcggtagtt ggcccgattg cctttatcgg cctgatgatg
    15301 ccgcacatgg cgcgctggct ggtaggtgcc gatcatcgct ggtcgctgcc cgtcacgcta
    15361 cttgctaccc ctgccctgct gctgtttgcc gatatcatcg ggcgggtgat tgtgcccggc
    15421 gaactgcgcg tttctgtggt cagtgcgttt attggtgcac cggtgctgat cttcctcgtg
    15481 cgacgtaaaa cgcgaggtgg tgcatgattt acgtctctcg ccgattactc atcacctgtt
    15541 tgctgctgat ttccgcctgt gtggttgcag gtatctgggg attacgcagc ggtgccgtca
    15601 cgctggaaat ctcgcaggta ttcgccgcgt tgatgggcga tgcgccgcgc agtatgacga
    15661 tggtggtcac cgaatggcgt ttaccacgcg tgctgatggc gctgttgatt ggcgcggcgc
    15721 tgggcgtcag cggcgcgatt tttcagtcgc tgatgcgtaa cccgctcggc agccctgacg
    15781 taatgggctt taacaccggg gcgtggagcg gcgtgctggt ggcgatggtg ctgtttggtc
    15841 aggacctgac ggctatcgcc ctggcagcaa tggtgggcgg cattgtcact tcgctgttgg
    15901 tctggctgct cgcctggcgt aacggtatcg acacctttcg gttgattatt atcggcatcg
    15961 gcgttcgcgc catgctggtg gcctttaata cctggctgtt gctgaaagcg tctttagaaa
    16021 cggcgctaac agcgggtttg tggaatgcag gatcgctcaa cggcctgacg tgggcaaaaa
    16081 cctcgccttc cgcacccatc attatattga tgctcattgc cgccgcctta ctggtacgcc
    16141 ggatgcgctt gctggaaatg ggcgatgata ccgcgtgtgc gctgggcgtc agcgtcgaac
    16201 gttcgcgtct gttaatgatg ctggttgcag tggtgcttac cgctgcggca accgcgcttg
    16261 ccgggccgat ttcctttatt gctttagtcg cgccgcacat ttcccgacgc attagcggca
    16321 ccgctcgctg ggggctaacc caggcggcgc tatgcggggc gctgttactg ctggcggccg
    16381 atctctgcgc ccaacaactg tttatgccgt atcaacttcc ggttggcgtc gttaccgtca
    16441 gcctcggcgg tatttacctt atcgtcttgt taattcagga gtctcgcaaa aaatgaccga
    16501 atcagtagcc cgtttgcgcg gcgaacagtt aaccctggga tatggcaaat ataccgttgc
    16561 ggagaatctg acagtagaaa tacctgatgg tcacttcacg gcaattatcg ggccaaatgg
    16621 ctgcggtaaa tccactttac tgcgtacctt aagccgcctg atgacgcctg ctcacggaca
    16681 tgtctggctg gatggcgagc acatccaaca ttacgccagt aaagaggttg cacgccggat
    16741 tggtctgttg gcgcaaaatg ctaccacgcc gggcgatatc accgtgcagg agctggtggc
    16801 gcgtgggcgt tacccgcatc aaccgctgtt tacccgctgg cgcaaagaag atgaagaagc
    16861 ggtaacgaaa gcgatgcagg ccacgggaat aactcatctg gcggatcaaa gcgtggatac
    16921 cctttctggc ggacaacgcc agcgcgcgtg gatcgcgatg gtgctggccc aggaaacggc
    16981 aattatgctg ctcgacgaac ccacgacctg gctggatatc agtcatcaga ttgatctact
    17041 ggaactgtta agcgaactga accgcgagaa aggctatacc ctggcggcgg tgctgcacga
    17101 tcttaatcag gcctgtcgtt acgccagcca tttgattgca ttgcgggaag ggaaaattgt
    17161 tgctcaggga gcgccgaagg agattgtcac tgctgaactg attgagcgca tttatggtct
    17221 gcgatgcatg atcattgacg atccggtagc cggaacgccg cttgtggtgc cgctcggacg
    17281 aacggcacct tcaaccgcaa atagttaaac taagtggtct gccatcatgg catcctgttt
    17341 tctggatgcc atcgcatagc gcaataacac gctaccacaa gccaccatcc cgccgatcag
    17401 cgcggaaagg atcacaataa tcgccttacc cggaccgtct tttttcactg gcaatgacgg
    17461 gcttaactga tatttaaacg gcgtaaaatt cacatcgttg atatttgctt ttgttaactg
    17521 ctcaacaaga tactgacgat tacgtaattc accgttcagt tccgcaacgt cagtgaccgc
    17581 tttttcaatt tccagtttgc gttcaatacc gtctgcgccg agagaaatag agaaatcggg
    17641 gtcatcttta actgcctgac cattactgta tacgggcttt ttaattcccg ccgcgttggc
    17701 aatgtcgagt gaataattga ggcgctgaat gtttgcatca agttgatttt tcgttttaat
    17761 gcgatcctga gccagttttt ctttttcaaa ctgggttttg atttccagtt tattacggac
    17821 gttttctagt gactctttca ccaccaacgc agagatataa tcgatatacc cgctcaaaac
    17881 ggtctgcgcc tcttcactgg ttggcgcggt aaaacttagc gtccaggagg tatacagtga
    17941 cggttcatct tttttcttac tggcattgtc atcaaccgct ttcatttttt cgctcaatgc
    18001 aacaatagcg cgatgcaaat ccagttcatc gattttcgcc tcttttaatt ggtccatcac
    18061 ataaggtgat gaacgcaggt actcttccag caagctaacc gactgaaact tcttgataaa
    18121 caggttaaat gcttctgtac gatcaatttt gatatccaga tccagcactc gaagcttagt
    18181 gaatgttttc tccagctctt gccactgaac aggttctgga ggcgtgacaa ccgccgcgct
    18241 ggtccatttt tgcggcagga tgaaagagat cagcaagcct gcgcaggcaa acgcaaaaat
    18301 gaccgccatg accgtttttt tggcccgcca taaaactgcg attagattaa gtaaatcaat
    18361 ttcattatta ctgggcgacg ccagaggata ctcaggaaaa tgagcgtcac ttccctgttt
    18421 aatattcagt gatgacatgg ggtataacct gaaaatgagt ccaatagggg aaatctccac
    18481 ggcgctcact ttaccagttg gtgataatta tccgaagctg aagtttgtaa attccttcca
    18541 ctgaatgcaa cattcactct atttaagaga aatattcatc aaacctataa atcaaacaaa
    18601 taaaaaaacc c
//
LOCUS       DAZKIV010000090        18593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZKIV010000090 DAZKIV010000000
VERSION     DAZKIV010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.090"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACHOT6_003004"
     CDS             complement(54..2123)
                     /gene="glyS"
                     /locus_tag="ACHOT6_003004"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFP9948184.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACHOT6_003005"
     CDS             complement(2133..3044)
                     /gene="glyQ"
                     /locus_tag="ACHOT6_003005"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFP9948185.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3139..3438)
                     /locus_tag="ACHOT6_003006"
     CDS             complement(3139..3438)
                     /locus_tag="ACHOT6_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFP9948186.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3613..4608
                     /gene="wecH"
                     /locus_tag="ACHOT6_003007"
     CDS             3613..4608
                     /gene="wecH"
                     /locus_tag="ACHOT6_003007"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFP9948187.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALNNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4650..5087)
                     /gene="yiaA"
                     /locus_tag="ACHOT6_003008"
     CDS             complement(4650..5087)
                     /gene="yiaA"
                     /locus_tag="ACHOT6_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFP9948188.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5133..5474)
                     /gene="yiaB"
                     /locus_tag="ACHOT6_003009"
     CDS             complement(5133..5474)
                     /gene="yiaB"
                     /locus_tag="ACHOT6_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFP9948189.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5643..7097)
                     /gene="xylB"
                     /locus_tag="ACHOT6_003010"
     CDS             complement(5643..7097)
                     /gene="xylB"
                     /locus_tag="ACHOT6_003010"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFP9948190.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7169..8491)
                     /gene="xylA"
                     /locus_tag="ACHOT6_003011"
     CDS             complement(7169..8491)
                     /gene="xylA"
                     /locus_tag="ACHOT6_003011"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFP9948191.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGMLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8857..9848
                     /gene="xylF"
                     /locus_tag="ACHOT6_003012"
                     /pseudo
     CDS             8857..9848
                     /gene="xylF"
                     /locus_tag="ACHOT6_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
     gene            9926..11467
                     /gene="xylG"
                     /locus_tag="ACHOT6_003013"
     CDS             9926..11467
                     /gene="xylG"
                     /locus_tag="ACHOT6_003013"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFP9948192.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAEEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11445..12626
                     /gene="xylH"
                     /locus_tag="ACHOT6_003014"
     CDS             11445..12626
                     /gene="xylH"
                     /locus_tag="ACHOT6_003014"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFP9948193.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVMGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12704..13882
                     /gene="xylR"
                     /locus_tag="ACHOT6_003015"
     CDS             12704..13882
                     /gene="xylR"
                     /locus_tag="ACHOT6_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFP9948194.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(13990..14814)
                     /locus_tag="ACHOT6_003016"
     CDS             complement(13990..14814)
                     /locus_tag="ACHOT6_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFP9948195.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15134..17164
                     /gene="malS"
                     /locus_tag="ACHOT6_003017"
     CDS             15134..17164
                     /gene="malS"
                     /locus_tag="ACHOT6_003017"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFP9948196.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASSLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17342..>18593
                     /gene="avtA"
                     /locus_tag="ACHOT6_003018"
     CDS             17342..>18593
                     /gene="avtA"
                     /locus_tag="ACHOT6_003018"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFP9948197.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
BASE COUNT         4689 a         4720 c         4566 g         4618 t
ORIGIN      
        1 tagcgataat aaaaaaagcc tgccagatgg caggcttttt aataacggcg ttattactgc
       61 aacagcgaaa tatccgcaac gcgcaggaac agttcgcgca gtttctccag catggtcaga
      121 cggttgagac gcaattcttt gtcatcaacc atgaccatca ctttatcgaa gaaagcatca
      181 accggctcac gcagctctgc cagttcgacc agcgcatcct ggtaacgacc ttccgcaaag
      241 tacggctcca gcttgtcacg tagcaccaca acctgcatcg ccagtttaat ttcttccggc
      301 tctttcaggg tagaggcatt cacgcggtcg ctcagcactt cgtcagattt cgccagaatg
      361 ttagatacac gtttgttcgc cgccgccagt gcagcagctg catccagggt acggaaatgc
      421 gataccgctt tcatacgggc atcgaaatca gccggacgag tcggacgacg cgccagtacc
      481 gcctggatgg tgtcaacggt ataaccttcg tcctgatacc aggcgcggaa gcgaccgagc
      541 ataaagtcga taacatcatc aactacgttg gcattagtca gcttatcgcc atacagacgc
      601 accgcttctt cggtcagcgt ttgcagatca aggttgaggt tcttctcaac gataattcgc
      661 agcacgccaa gtgcggcacg acgcagcgca aacgggtctt tgtcgccttt cggatgctgg
      721 ccgataccga agatacccgc cagagtatcc atcttgtcag caatcgccag cgcacaggct
      781 accgggttag acggcaggtc atcaccggca aagcgcggct gatactgctc attcagcgcc
      841 accgcgacat cttccgcttc gccatcgtga cgcgcatagt gcatccccat aacgccctgg
      901 gtgtcggtga actcgaagac catgttggtc atcaggtcac acttagacag caggcccgca
      961 cgggttgcgt ggttaacgtc agcgccaatc tgttcagcaa tccagccagc cagcgcctgg
     1021 atgcggtcag ttttgtcgcg cagcgtaccc agctgttgct ggaacagcac ggtttgcagg
     1081 cgcggcaggt tatcttccag acgtttttta cggtcggtat tgaagaagaa ctcggcatcc
     1141 gccagacgtg gacgaacgac tttctcgtta ccagagataa tttgctgcgg atctttcgat
     1201 tcgatgttgg caacgaagat gaagttcggc agcagtttgc catcgttcgc atacaccggg
     1261 aagtatttct ggtcaccttt catggtgtaa accagggctt cagccggcac cgcgaggaat
     1321 ttctcttcga attttgcggt cagaacgacc ggccactcca ccagcgaagc cacttcttcc
     1381 agcaggcttt cgcttaagtc agcgttaccg ccaatcttac gcgctgcttc ttcggcatcg
     1441 gctttaatct tcgctttacg ttcttcgtaa tcggcgatga ctttcccgcg ctcacgcaga
     1501 atttccggat actgatcggc gttgtcgatg gtgaattccg gttcgcccat aaagcggtgg
     1561 ccgcgaatca cgcgatcgga ctgaatgccc agaatggttg ccggaatgac tttgtcgccc
     1621 agcagcaggg tcacggtgtg caccggacgc acgaagtgca cgtcgcttgc gccccaacgc
     1681 atcagtttcg ggatcggcag tttcgccaga gaagtcgcaa ccatattcgg cagcagtgct
     1741 tcggtgcttt cgcccttcac atgggcgcga tacagcagcc attcgccttt atcggtagtc
     1801 agacgctcag cctggtcaac ggtaataccg caaccacgcg cccaaccttc tgccgctttg
     1861 ctcggtttgc cttcagcgtc gaacgcctgg gcaatcgccg ggccgcgttt ttcgatttca
     1921 cgatccggtt gcgcttccgc caggttagct actttcagcg ccagacgacg cggagcagca
     1981 aaccattgaa cggtgccgtg tgcgaggcca gcgttatcca gctccgcagt aaaattcgca
     2041 gcaaaggact cagccaggct gcgcagtgct tttggtggca gctcttcagt gccgatttcc
     2101 accagaaaag ttttctcaga catagccgcc tcttacttat ctttgttgca catcgggaag
     2161 ccgagggctt cacgggaagc gtagtatgct tctgccactg ctttggtcag ggtgcgaatg
     2221 cgcagaatgt agcgctgacg ctcggtgacg gagatggctt tacgcgcatc cagcaggttg
     2281 aagctgtggg cggctttcag aatacgctcg taggctggca gcggcagcgg attttccagc
     2341 gccagcagct gctgcgcttc tttctcgtac tgctcaaagc aggtgaacag gaagtccaca
     2401 tccgcgtatt cgaagttgta agtggactgc tccacttcgt tctgatggaa cacgtcgccg
     2461 taggtggttt tacccagcgg gccgtcgctc cagaccaggt cgtaaacgct gtctacgccc
     2521 tgaatgtaca tggccagacg ttccagaccg taggtgatct cgccggtaac cggtttacac
     2581 tccagaccac caacctgctg gaagtaagtg aactgcgtca cttccatgcc gtttagccac
     2641 acttcccagc ccagtcccca ggcacccaga gtcgggtttt cccagttatc ttccacgaaa
     2701 cggatatcgt gaatagtcgg gtccatgccc agctctttca gagaaccgag gtacagctcc
     2761 tgaatattgt ccggcgatgg cttaatgacc acctggaact gatagtagtg ctgtaaacgg
     2821 ttggggtttt cgccgtagcg accatcggtc gggcgacgag aaggctgaac ataagcagcc
     2881 gccatcggtt ctggccccag cgcgcgcaga caggtcattg ggtgagaggt tcccgcgccg
     2941 acttccatgt ccaatggttg aacaatggtg cagccctggc gagcccagta atcctgtaag
     3001 gtcaggatca agccctggaa ggtcctggta tcaaactttt gcatattatt tcgtgctgga
     3061 tacgtgtgga tttaaaggaa gggatcagta tacccgctgg atggaagata tacagtacga
     3121 aacgggaaaa agcagggctt aacgcatgga aagatgcaaa aaatggccat ctgcgtcaaa
     3181 agaacagaca aactgctctt tacggaaggt ataaccgcgc atttcatagc ttccctggaa
     3241 ctgttcgaag gcggtgacgt cgattttttg ctcgccggtg ttataacgtt ttgccgcctg
     3301 atccttgcat aacgcttcca tattcaacga acgcagagga tcaaccttta cccgttgtgc
     3361 tttctgcacg ggtgacggtg tagaacaacc cactagcacc ataagcgcca ttgccggaaa
     3421 gaaagcgttc atcatcatta aattaccgtc tggtttgcag gtgagtctta ttatttatat
     3481 cggtatagtg gctgatatct tacgaatttc cttaagcctc tataagctgt agtgataaaa
     3541 ctcagattat tccagtgcgc caaccaaatg atttatcggt cagacttttt ttaaccagat
     3601 acagagacac agatgcagcc caaaatttac tggattgata acctgcgagg gatagcgtgt
     3661 ttaatggtgg tgatgattca caccactacc tggtatgtga ccaatgctca tagtgttagc
     3721 cccgtcacat gggatatcgc caatgttctg aattctgcct ctcgtgtcag cgtgccgcta
     3781 tttttcatga tttccggcta tctctttttt ggcgaacgca gcgcccagcc gcgccatttc
     3841 ttgcgtatcg gcttatgtct gattttttat agcgcaatcg cactgctcta cattgcgctg
     3901 tttacctcca tcaatatgga gttagcgctg aacaacctgc tgcaaaagcc agtgttttac
     3961 cacttgtggt ttttcttcgc gattgcggtg atttatctgg tttcaccgct gattcaggtg
     4021 aagaacgtcg gcggaaaaat gttgctggtg ctaatggcgg tgattggcat tatcgctaac
     4081 ccaaacacag tgccgcagaa aattgacggt tttgaatggc tgccaattaa cttatatatc
     4141 aatggcgata ctttttacta cattctgtat ggcatgttgg gccgcgctat agggatgatg
     4201 gacacacagc ataaagcact gtcgtgggtg agcgccgcgc tgtttgcgac gggggttttt
     4261 attatctctc gcgggacatt atatgaattg cagtggcgcg gaaattttgc cgatacctgg
     4321 tatctttact gtgggccgat ggtttttatc tgcgcaatcg cgctattgac tctggttaaa
     4381 aacacgctgg atacgcgtac cattcgcgga cttggcttaa tctcccgcca ttcattgggt
     4441 atatacggat tccacgcctt gattatccat gcgctgcgca cccggggaat tgagcttaaa
     4501 aattggccaa tactggatat tatttggatc ttttgcgcga cgttggcagc gagtttgtta
     4561 ctttctatgc tggtacaacg aatcgacaga aacagattag tgagttaagt aaaagcccgt
     4621 tcacattgga ctgaccgggc ttacgtgaat tattcgctgt attcttcctg ggtaacctgt
     4681 gtttcttttg gcgggtttat tccggcatca cgaatattct tctgcaccgc tactgcacca
     4741 aacaagctta agaagaaagc cagtccataa aaaccttttt cactgagtaa taatgtcgca
     4801 ttccacagac ctaccatcag taatgcaaca gagataataa agacagtcag gcaggtcata
     4861 taataaatgg aagtggtcgg tatgccttca tacttgtccc gaacggtctt ttgataagac
     4921 gcagcagaaa acagtcctaa caccagtacg gcaaaataat accctttttc atttaactgc
     4981 atctctgcat tccatagccc taacagatag gtaacgatac caccaacgag agctatccat
     5041 gacacaatac taaaggccgg tgaataggtt gatattttgt tgtccatcac agtattcctt
     5101 tcattcctga atgtgtaaga gctttatgtt gctcataccg agcgaaacaa acgcatttga
     5161 ccaaacaagc agacaaaaaa ggcgatgggg tagatcacca tttcataagc attgataggt
     5221 gcatttaaaa caccgataaa caagagaccg atagtgatta acgcaactaa ctggcagata
     5281 tgggtaaaaa aattatccag ttgcccgagt ttttctgcgc gaagatatgc atagttacca
     5341 aaagttgctg tcattaacac gccaagaaaa tagccttttc cacttagcaa tgggcatgag
     5401 atccatagcc caaccagata aaccagcgcc ccgacaacaa ataatagttt tgccacagtt
     5461 tttgaggttt tcataacgat ctccatatct accagcgata cattacgagt aaccaacgaa
     5521 agacaaaact gaaaaatgcc attaacaaat gattttcaga ataaattcat actaaatatt
     5581 aattaattag tgagatatat agatgtgaat tatcccccac ccggtcaggc aggggataac
     5641 gtttacgcca ttaatggcag aagttgctga tagaggcgac ggaacgtttc acgtcgtggc
     5701 tgataagcgg cataacgctg cgcatctggt agatgcgact gttctaacgg tagctgcggc
     5761 aacaactcaa tgagcgattt ctctggattc gccgcaatct gcgccagcct tgctgcgccc
     5821 agtgctggcc ccacatcccc ccccgtacgg taatcgagct gctgaccgct gatatccgcc
     5881 agcatctgac gccagtactc actacgcgcc ccgccaccaa tcaacgtaac actttgcggt
     5941 ttaatgccgc aggcatgcac gacatccatg ccatctgcca gcgcataacc cacgccttcc
     6001 agcactgctc gcgccagttc attggggcca tgttgatgag tcaaaccaaa gaaaaccccc
     6061 ttcgcctggg gattattgtg tggtgtacgc tcgccggaaa gataaggcag aaaccaaact
     6121 ggctcggcac tttcatcagc ctgttgagct gcagcgatta aagctgggac attgcacagg
     6181 ccggttaatt tcgcgaccca atccagacac gatgctgcac tcagcattac agacattaaa
     6241 tgccaacgct gcggtagcgc atggcaaaag ctgtgtacag cgctttctgg cttgcttaag
     6301 aacccttcgc tgacagcaaa atagaccccc gacgtcccca gcgataacat tgcctgatta
     6361 gcatcaacca ttcccacacc aactgcacca gctgcattgt cgccaccgcc tgcaacaact
     6421 ggcaccgtcg ccatacccca cgctttcgca acttcaggta acaaagcacc agtaatttcg
     6481 ctgccttcgt ataatgcggg catctggtca cgagataagt cgcaagcctg cagcatgacg
     6541 tcactccagt cacgctttgc gacatccagc cacatggtgc cagctgcgtc agacatatcg
     6601 ctggcaaact cccccgtcat acgcagacgc aagtaatctt tcggtaataa tactttgtcg
     6661 atttgacgga atatctccgg ctcatgccgc tgaacccata gcaatttagg cgcagtaaat
     6721 ccgggcatca tcaggttgcc ggtaatcact cgtgattgcg gaactctcgc ttccagcaaa
     6781 gtgcactctt gcgcacagcg cccgtcgttc cacaaaatgg cagggcgcaa tacccgttgt
     6841 tgagcatcca gtaaggttgc tccatgcatc tggccggcaa tacccaatgc tttaacgtcc
     6901 tgcagagaat gctgatcgcc cagagctttc attgcgcgat cagttgcctg ccaccactgt
     6961 tccgggtctt gttccgacca gagtggatgc gggcgcgaaa cggtcagctt ttccgtttgc
     7021 gaagcaacca cctcaccctg ctcgttgagc aaaataactt ttacgcccga ggtgccaaga
     7081 tctatcccga tatacatatc gatcgttcct taaaaaaatg cccggtatcg ctaccgataa
     7141 ccgggccaac ggactgcaca gttagccgtt atttgtcgaa cagataatga tttaccagat
     7201 tttccagttg ctcctggcga ccactctgat gcaccggaga caaattatgt tcctgagcat
     7261 atttggctaa atctgccagt gacatttggc ctttcaggat ttgctggccc aattcgctat
     7321 tccagccgga ataacgctgc gcgatgcgtt tatccagctc gccatcttca atcatgcgcg
     7381 ctgcaatttt cagcgccagt gccatcgtat ccatcgcgcc gatatgaccg taaaacagat
     7441 catatttatc agtactttga cgacgtactt tggcatcgaa gttcaaacca ccggtggtga
     7501 aaccgcctgc tttgagaatt tcatacatca ccagcgcatt ctcttccaca ctgttcggga
     7561 actggtcggt gtcccagccc agttgcgcat cgccacggtt ggcgtcgaca gaaccgaaca
     7621 ggccaagcgc aatggcggtg gctatttcat gatggaaaga gtgacctgcc agcgtcgcgt
     7681 ggttagcttc aatgttcagt ttaatctctt tttccagacc aaactgtttc aggaagccat
     7741 agaccgtcgc ggcatcgtaa tcatattgat gtttagtcgg ttcttgcggt ttcggttcga
     7801 taagcaacat gccctggaag ccgattttat gtttatgctc aaccaccatc tgcataaagc
     7861 ggcccagttg ttcacgctcc tgacgcaagt cggtatttaa cagcgtttcg taaccttcac
     7921 gaccacccca caggacatag ttttcaccgc ccaatttatg ggttgcttcc atcgctgtaa
     7981 caacttgcgt tgccgcccag ctgaagactt caggatctgg gttcgtcgcc gcacccgcgc
     8041 cgtagcgagg gtttgtaaag cagttggcgg ttccccacag cagcttcacg ccgctctctt
     8101 cttgcttgcc tgccaggaca tcaaccattt gcgcaaaatt attgatgtac tcttttaacg
     8161 acgcgccctc aggggaaaca tccacatcgt ggaagcaata aaatggcaca tgtaacttgt
     8221 ggaaaaactc aaatgcgaca tctgctttac gcttcgccaa cgccagtgcc tcaccaggct
     8281 gctgccacgg acgattaaac gcccccacac caaacatgtc cgccccgttc cagcagaagg
     8341 tgtgccagta gcaggcggca aaacgcaagt gctcttccat acgcttaccc aacaccagtt
     8401 cgtcgggatt gtagtgacgg aatgctaacg ggtttgagga ttttgagcct tcataacgaa
     8461 cgcgatcgag ctggtcaaaa taggcttgca tattgaactc cataatcagg taatgccgcg
     8521 ggtgatggat gatgtcgtaa tattgggcac tccctttcag ttgctcaatt atgttatttc
     8581 acactgctat tgagataatt cacaagtgtg cgctcgctcg caaaataaaa tgggatgatg
     8641 aaactgggta attcctcgaa gataaaaatg caataagtac aattgcgcaa caaaagtaag
     8701 atctcggtca taaatcaaga aataaaccaa aaatcgtaat cgaaagataa aaatctgtaa
     8761 ttgttttccc ctgtttagtt gctaaaaatt ggttacgttt atcgcggtga ttgttactta
     8821 ttaaagctgt cctctaacta cagaaggccc tacaccatga aaataaagaa cattctactc
     8881 accctttgca cctcacttct gcttaccaac gttgcggcac acgccaaaga agtcaaaata
     8941 ggtatggcga ttgatgatct ccgtcttgaa cgctggcaaa aagatcgaga tatttttgtg
     9001 aaaaaggcag aatctctcgg cgcgaaagta tttgtacagt ctgcaaatgg caatgaagaa
     9061 acacaaatgt cgcagattga aaacatgatt aaccggggtg tcgatgttct tgtcattatt
     9121 ccgtataacg gccaggtatt aagtaacgtt gtaaaagaag ccaaacaaga aggtattaaa
     9181 gtattagctt acgaccgtat gattaacgat gcggatatcg atttttatat ttctttcgat
     9241 aacgaaaaag tcggtgaact gcaggcaaaa gccctggtcg atattgttcc gcaaggtaat
     9301 tacttcctga tgggcggctc gccggtagat aacaacgcca agctgttccg cgccggacaa
     9361 atgaaagtgt taaaacctta cgttgattcc ggaaaaatta aagtcgttgg tgaccaatgg
     9421 gttgatggct ggttaccgga aaacgcattg aaaattatgg aaaacgcgct aaccgccaat
     9481 aataacaaat tgatgctgta gttgcctcaa acgatgccac cgcaggtggg gcaattcagg
     9541 cattaagcgc gcaaggttta tcagggaaag tagcaatttc cggccaggat gcggatctcg
     9601 caggtattaa acgtattgct gccggtacgc aaactatgac ggtgtataaa cctattacgt
     9661 tgttggcaaa tactgccgca gaaattgccg ttgagttggg caatggtcag gaaccaaaag
     9721 cagataccac actgaataat ggcctgaaag atgtgccctc ccgcctcctg acaccgatcg
     9781 atgtgaataa aaacaacatc aaagacacgg tagttaaaga cggattccac aaagagagcg
     9841 agctgtaagc gttacgcccc ggcgcggagc gggggcgtga tttctctcca tgccgcgtga
     9901 atgaattggc ttaggtggag tcgttatgcc ttatctactt gaaatgaaga acattaccaa
     9961 aaccttcggc agtgtgaagg cgattgataa cgtctgcttg cggttgaatg ctggcgaaat
    10021 cgtctcactt tgtggggaaa atgggtctgg taaatcaacg ctgatgaaag tgctgtgtgg
    10081 tatttatccc catggctcct acgaaggcga aattattttt gcgggagaag agattcaggc
    10141 gagtcacatc cgcgataccg aacgcaaagg tatcgccatc attcatcagg aattggccct
    10201 ggtgaaagaa ttgaccgtgc tggaaaatat cttcctgggt aacgaaataa cccacaatgg
    10261 cattatggat tatgacctga tgacgctacg ctgtcagaag ctgctcgcac aggtcagttt
    10321 atccatttca cctgataccc gcgttggcga tttagggctt gggcaacaac aactggttga
    10381 aattgccaag gcacttaata aacaggtgcg cttgttaatt ctcgatgaac cgacagcctc
    10441 attaactgag caggaaacgt cggttttact ggatattatt cgcgatctac aacagcacgg
    10501 tatcgcctgt atttatattt cgcacaaact caacgaagtc aaagcgattt ccgatacgat
    10561 ttgcgttatt cgcgacggac agcacattgg tacgcgtgat gctgccggaa tgagtgaaga
    10621 cgatattatc accatgatgg tcgggcgaga gttaaccgcg ctttacccta atgaaccaca
    10681 taccaccgga gatgaaatat tacgtattga acatctgacg gcatggcatc cggtcaatcg
    10741 tcatattaaa cgagttaatg atgtctcgtt ttccctgaaa cgtggcgaaa tactgggtat
    10801 tgccggactc gttggtgccg gacgtaccga gaccattcag tgcctgtttg gcgtgtggcc
    10861 cggacaatgg gaaggaaaaa tttatattga tggcaaacag gtagatattc gtaactgtca
    10921 gcaagccatc gcccagggga ttgcgatggt acccgaagac agaaagcgcg acggcatcgt
    10981 tccggtaatg gcggttggta aaaatattac cctcgccgca ctcaataaat ttaccggtgg
    11041 cattagccag cttgatgacg cggaagagca aaaatgtatt ctggaatcaa ttcagcaact
    11101 caaagttaaa acgtcgtccc ccgatcttgc tattggacgt ttgagcggcg gcaatcagca
    11161 aaaagcgatc ctcgcccgct gtctgttact caacccgcgc attctcattc ttgatgaacc
    11221 taccaggggt attgatattg gcgcgaaata tgagatctac aaattaatta accaactcgt
    11281 ccagcagggt attgccgtta ttgtcatctc ttccgaatta cctgaagtgc tcggccttag
    11341 cgatcgtgta ctggtgatgc atgaagggaa actaaaagcc aacctgataa atcataacct
    11401 gactcaggag caggtgatgg aagccgcatt gaggagcgaa catcatgtcg aaaagcaatc
    11461 cgtctgaagt gaaattggcc gtaccgacat ccggtggctt ttctgggctg aaatcactga
    11521 atttgcaggt cttcgtgatg attgcagcta tcatcgcaat catgctgttc tttacctgga
    11581 ccaccgatgg tgcctactta agcgcccgta acgtctccaa cctgttacgc cagaccgcga
    11641 ttaccggcat cctcgcggta ggaatggtgt tcgtcataat ttctgctgaa atcgaccttt
    11701 ccgtcggctc aatgatgggg ctattaggtg gcgtcgcggc gatttgtgac gtctggttag
    11761 gctggccttt gccacttacc atcattgtga cgttggttat gggactgctt ctcggtgcct
    11821 ggaacggatg gtgggtcgcg taccgcaaag tcccttcatt tattgtcacc ctcgcgggca
    11881 tgttggcatt tcgcggcata ctcattggca tcaccaacgg cacgactgta tcccccacca
    11941 gcgccgcgat gtcacaaatt gggcaaagct atctccccgc cagtaccggc ttcatcattg
    12001 gcgcgcttgg cttaatggct tttgttggtt ggcaatggcg cggaagaatg cgccgtcagg
    12061 ctttgggttt acagtctccg gcctctaccg cagtagtcgg tcgccaggct ttaaccgcta
    12121 tcatcgtatt aggcgccatc tggctgttga atgattaccg tggcgttccc actcctgttc
    12181 tgctgctgac gttgctgtta ctcggcggaa tgtttatggc aacgcggacg gcatttggac
    12241 gacgcattta tgccatcggc ggcaatctgg aagcagcacg tctctccggg attaacgttg
    12301 aacgcaccaa acttgccgtg ttcgcgatta acggattaat ggtagccatc gccggattaa
    12361 tccttagttc tcgacttggc gctggttcac cttctgcggg aaatatcgcc gaactggacg
    12421 caattgcagc atgcgtgatt ggcggcacca gcctggctgg cggtgtggga agcgttgccg
    12481 gagcagtaat gggggcattt atcatggctt cactggataa cggcatgagt atgatggatg
    12541 taccgacctt ctggcagtat atcgttaaag gtgcgattct gttgctggca gtatggatgg
    12601 actccgcaac caaacgccgt tcttgatttt gataaaaatt ttctcaaagc cggttacgta
    12661 ttaccggttt tgagtttttg catgattcag caggaaaaga accatgttta ctaaacgtca
    12721 ccgcatcaca ttactgttca atgccaataa agcctatgac cggcaggtag tagaaggcgt
    12781 aggggaatat ttacaggcgt cacaatcgga atgggatatt ttcattgaag aagatttccg
    12841 cgcccgcatt gataaaatca aggactggtt aggagatggc gtcattgccg acttcgacga
    12901 caaacagatc gagcaagcgc tggctgatgt cgacgtcccc attgttgggg ttggcggctc
    12961 gtatcacctt gcagaaagtt acccacccgt tcattacatt gccaccgata actatgcgct
    13021 ggttgaaagc gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg ctttttatgg
    13081 tcttccggaa tcaagcggca aacgttgggc cactgagcgc gaatatgcat ttcgtcagct
    13141 tgtcgccgaa gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg cgccagagaa
    13201 ctggcaacac gcgcaaaatc ggctggcaga ctggctacaa acgctgccac cgcaaaccgg
    13261 gattattgcc gttactgacg cccgagcgcg gcatattctg caagtatgtg aacatctaca
    13321 tattcccgta ccggaaaaat tatgcgtgat tggcatcgat aacgaagaac tgacccgcta
    13381 tctgtcgcgt gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg gctatcaggc
    13441 ggcaaaactg ttgcatcgat tattagataa agaagaaatg ccgctacagc gaattttggt
    13501 cccaccagtt cgcgtcattg aacggcgctc aacagattat cgctcgctga ccgatcccgc
    13561 cgttattcag gccatgcatt acattcgtaa tcacgcctgt aaagggatta aagtggatca
    13621 ggtactggat gcggtcggga tctcgcgctc caatcttgag aagcgtttta aagaagaggt
    13681 gggtgaaacc atccatgcca tgattcatgc cgagaagctg gagaaagcgc gcagtctgct
    13741 gatttcaacc accttgtcga tcaatgagat atcgcaaatg tgcggttatc catcgctgca
    13801 atatttctac tctgttttta aaaaagcata tgacacaacg ccaaaagagt atcgcgatgt
    13861 aaatagcgag gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc agggaactca
    13921 gcacagccac ctgatgcgag caaaagcctt atctgaccga caaatcagat aaggctcaag
    13981 gaaatgcaat tacatatgcg ccgcgattaa ccgttggtta tcctggtaca ttgcgaacag
    14041 gtagttatta taactcttcc ctttggtcga gtagcccttc agcttgtgaa tcatcgctgt
    14101 ggcagtcact tcctgatccg ctttacgcag ctgcgcacgc gatttacgga acgaagagta
    14161 agccgggtgc gtattcaggt tagtgacata ggcgctcacc gattctttga cagaactaaa
    14221 ctgtgagtac cctttcactt tacccggcgc attggtacaa cgtcctttca tgcatttcat
    14281 gccgaacaga ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc tttctgctgc
    14341 agccatcgtc gccaccatac tggtagggat aatgtctacg cgttcaagca aggtattcca
    14401 cgggattttt cgcgtattac cagaccactt caccttgtag cgtttggcga tgtctttcag
    14461 acgcgcacgc tcagcaggtg accattggcc ctgatactgt tttgaaatca gccagttacg
    14521 ttctgcagta atggctgcat tttggctggt aatgtaaggc attacggtcc ggagaaacgc
    14581 ctttttcctt ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac tactctttgc
    14641 actattgcga gaatactctt gtttactgct tacctgttta ttactagctt tagttaagtg
    14701 ggacttttga ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg aaaacaccag
    14761 agtgagtaac ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa tcatcaggtc
    14821 tcctggtcgg atttaatcat tccaacacct tatatttttc acaaatttga gagttgaatc
    14881 tcaaatcata tcaaaaatag ctgtcaagag caccccaagg aatagtccaa atctgaaact
    14941 atgtcacgtg ttaacgattc agattggcgc taaatcgcag aaaatgtggg gtttatcgca
    15001 aaattcagcc gttttttgcg cgagatcgct cacccttgct tctcatcctg tggacttacc
    15061 gctcagggat gagttttgtc tggcttatcg ctggcaaact gtctgaaatc gcagcaataa
    15121 ggactcatcc accatgaaac tcgccgcctg ttttctgaca ctccttcctg gcttcgccgt
    15181 tgccgccagc tggacttctc cggggttccc tgcctttagc gaacagggaa cgggaacatt
    15241 tgtcagccac gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt ttgaccaaca
    15301 gtgctggcag cctgcagatg cgataaaact caatcagatg ctttccctgc aaccttgtag
    15361 caacacgccg cctcaatggc gattgttcag agacggcaaa tatacgctgc aaatagacac
    15421 ccgctccggt acgccaacat tgatgatttc catccagaac gccgccgaac cggtagcaaa
    15481 cctggtccgt gaatgcccga aatgggatgg attaccgctc acgctggatg tcagcgccac
    15541 tttcccggaa ggagccgccg tacgggatta ttacagccag caaattgcga tagtgaagaa
    15601 cggtcaaata acgttacaac ccgctgctac cagcaacggt ttactcctgc tggaacgggc
    15661 agaaactgac gcccctgccc ctttcgactg gcataacgcc acggtttact ttgtgctgac
    15721 agatcgtttc gaaaacggcg atcccagtaa tgaccagagt tacggacgtc ataaagacgg
    15781 tatggcggaa attggcactt ttcacggcgg cgatttacgc ggcctgacca acaaactgga
    15841 ttacctccag cagttgggcg ttaatgcttt atggataagc gccccatttg agcaaattca
    15901 cggctgggtc ggcggcggta caaaaggcga tttcccgcat tatgcctacc acggttatta
    15961 cacacaggac tggacgaatc ttgatgccaa tatgggcaac gaagccgatc tacggacgct
    16021 ggttgatagc gcacatcagc gcggtattcg tattctcttt gatgtcgtga tgaaccacac
    16081 cggctatgcc acgctggcgg atatgcagga gtatcagttt ggcgcgttat atctttctgg
    16141 tgacgaggtg aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg ccgccgggca
    16201 aacctggcat agctttaacg attacattaa tttcagcgac aaaacaggct gggataaatg
    16261 gtggggaaaa aactggatca gaacggatat cggcgattac gacaatcctg gattcgacga
    16321 tctcactatg tcgctggcct ttttgccgga tatcaaaacc gaatcaacta ccgcttctag
    16381 tctgccggtg ttctataaaa acaaaacgga tactcacgct aaagtcatcg aaggctttac
    16441 acctcgcgat tacttaaccc actggttaag tcagtgggtc cgcgactatg ggattgatgg
    16501 ttttcgggtc gataccgcca aacatgttga gttgcccgcc tggcagcaac tgaaaaccga
    16561 agccagcgcc gcgcttcgcg aatggaaaaa agctaacccc gacaaagcat tagatgacaa
    16621 acctttctgg atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg actactatcg
    16681 ccacggcttc gatgcgatga tcaatttcga ttatcaggag caggcggcga aagcagtcga
    16741 ttgtctggcg cagatggata cgacctggca gcaaatggcg gagaaattgc agggtttcaa
    16801 cgtgttgagc tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg gcgacaaagc
    16861 agcagagtta ttactattag cgccaggcgc ggtacaaatc ttttatggcg atgaatcctc
    16921 gcgtccgttc ggtcctacag gttctgatcc gctgcaaggt acacgttcgg atatgaactg
    16981 gcaggatgtt agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa tcagccagtt
    17041 ccgcgcccgc catcccgcaa ttggcgcggg caaacaaacg acactttcgc tgaagcaggg
    17101 ctacggcttt gttcgtgagc atggcgacga taaagtgctg gtcatctggg ctgggcaaca
    17161 gtgacttttc cggcttcccg ttcgtcagta cctcgggaag ccgccaacca ggataaaatg
    17221 tcagccctaa tcagcgttgc aggataaagc accgctcact ctccaacaga ccgatttgca
    17281 ccccagcaaa tgtagcgtta ttgttacctt ccttgctaca gagttcgaca gatatcccgc
    17341 tatgacattc tccctttttg gtgacaaatt tacccgccac tccggcatta cgctgctgat
    17401 ggaagatctg aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg gcggtaatcc
    17461 ggcgcagatc ccggaaatgc aggactactt ccagacgcta ctgaccgaca tgctggaaag
    17521 tggcaaagcg actgatgcac tgtgcaatta cgacggtcca caggggaaaa cggagctact
    17581 cacactgctt gccggaatgc tgcgcgagaa gttgggttgg gatatcgaag cacagaatat
    17641 tgcactaaca aacggcagcc agagcgcgtt tttctactta tttaatctgt ttgccggacg
    17701 ccgtgccgat ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat acattggcta
    17761 cgctgacgcc ggactggaag aagatctgtt tgtctctgcg cgtccgaata ttgaactgct
    17821 gccggaaggc cagtttaaat accacgtcga ttttgagcat ctgcatattg gcgaagaaac
    17881 cgggatgatt tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta ctgacgaaga
    17941 gttgctgaag cttgacgcgc tggcgaatca acacggcatt ccgctggtga ttgataacgc
    18001 ttatggcgtc ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat ggaatccgaa
    18061 tatcgtgctg tgcatgagtc tttccaagct ggggctgcct ggctcccgct gcggcattat
    18121 catcgccaat gaaaaaatca tcactgccat caccaatatg aacggcatta tcagcctggc
    18181 acctggcggt attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg atctgctgcg
    18241 cctgtctgaa acagtcatca aaccgtttta ctaccagcgt gttcaggaaa ctatcgccat
    18301 cattcgccgc tatttaccgg aagatcgctg cctaattcat aaaccagaag gagccatttt
    18361 cctctggcta tggtttaagg atttgcccat tacgaccgag caactctacc agcgcctgaa
    18421 agcacgcggc gtgctgatgg tgccggggca caacttcttc ccgggactgg ataaaccgtg
    18481 gccgcatacg catcaatgta tgcgcatgaa ctacgtacca gagccggaga aaattgaggc
    18541 gggggtgaag attctggcgg aagagataga aagagcctgg gctgaaagtc act
//
LOCUS       DAZKIV010000091        18546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZKIV010000091 DAZKIV010000000
VERSION     DAZKIV010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.091"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..1019)
                     /gene="pyrD"
                     /locus_tag="ACHOT6_003019"
     CDS             complement(9..1019)
                     /gene="pyrD"
                     /locus_tag="ACHOT6_003019"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFP9948198.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1130..1867)
                     /locus_tag="ACHOT6_003020"
     CDS             complement(1130..1867)
                     /locus_tag="ACHOT6_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFP9948199.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1833..2348)
                     /locus_tag="ACHOT6_003021"
     CDS             complement(1833..2348)
                     /locus_tag="ACHOT6_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948200.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2356..2898)
                     /locus_tag="ACHOT6_003022"
     CDS             complement(2356..2898)
                     /locus_tag="ACHOT6_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948201.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2910..3980)
                     /locus_tag="ACHOT6_003023"
     CDS             complement(2910..3980)
                     /locus_tag="ACHOT6_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948202.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTYSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3971..6569)
                     /locus_tag="ACHOT6_003024"
                     /pseudo
     CDS             complement(3971..6569)
                     /locus_tag="ACHOT6_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(6594..7295)
                     /locus_tag="ACHOT6_003025"
     CDS             complement(6594..7295)
                     /locus_tag="ACHOT6_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9948203.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            complement(7378..7920)
                     /locus_tag="ACHOT6_003026"
     CDS             complement(7378..7920)
                     /locus_tag="ACHOT6_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948204.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            8277..8852
                     /gene="ssuE"
                     /locus_tag="ACHOT6_003027"
     CDS             8277..8852
                     /gene="ssuE"
                     /locus_tag="ACHOT6_003027"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFP9948205.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYYWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            8845..9804
                     /gene="ssuA"
                     /locus_tag="ACHOT6_003028"
     CDS             8845..9804
                     /gene="ssuA"
                     /locus_tag="ACHOT6_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFP9948206.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNFGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            9801..10946
                     /gene="ssuD"
                     /locus_tag="ACHOT6_003029"
     CDS             9801..10946
                     /gene="ssuD"
                     /locus_tag="ACHOT6_003029"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFP9948207.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            10957..11748
                     /gene="ssuC"
                     /locus_tag="ACHOT6_003030"
     CDS             10957..11748
                     /gene="ssuC"
                     /locus_tag="ACHOT6_003030"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFP9948208.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            11745..12512
                     /gene="ssuB"
                     /locus_tag="ACHOT6_003031"
     CDS             11745..12512
                     /gene="ssuB"
                     /locus_tag="ACHOT6_003031"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFP9948209.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(12719..15331)
                     /gene="pepN"
                     /locus_tag="ACHOT6_003032"
     CDS             complement(12719..15331)
                     /gene="pepN"
                     /locus_tag="ACHOT6_003032"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFP9948210.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            15597..16799
                     /gene="pncB"
                     /locus_tag="ACHOT6_003033"
     CDS             15597..16799
                     /gene="pncB"
                     /locus_tag="ACHOT6_003033"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFP9948211.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            16969..18369
                     /gene="asnS"
                     /locus_tag="ACHOT6_003034"
     CDS             16969..18369
                     /gene="asnS"
                     /locus_tag="ACHOT6_003034"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFP9948212.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         4475 a         4736 c         4579 g         4756 t
ORIGIN      
        1 agaaataatt agatatgggt aacgatttct ttaatcagcg gcggaccttt aaaaataaaa
       61 ccagaataaa tttgcaccag tgaggcaccc gcagcaatct tttcacgcgc agcgataacc
      121 gagtcgatgc cgccaacacc gatgatcggt aagcgaccgt ttaattccag tgacaagcgg
      181 cgaataattt cggtgctttt taactgaagc ggacgaccac ttaagccacc ggtttgatcg
      241 caatttttca ttccctgaac aagagaacga tcgagtgtgg tattggttgc aataacgcca
      301 tcaatattat ggcgaactaa actatcggca acctggatca attcttcttc agaaagatcc
      361 ggcgcgatct tcactgcgat cggcacatat ttatggtgca tcgcttgcaa atcattttgc
      421 ttatttttaa tcgcggttaa gagatcatcc agcgcttcac catattgcag cgtgcgtaat
      481 cctggggtat tcggcgatga aatattgatg gcgatatatc ccgcataggc atagattttt
      541 tccatacaaa tcagatagtc atctttgccc tgctccactg gcgtatcttt atttttgccg
      601 atgttaatac ccaggacgcc gtcataatgg gcctttttta cgttctctac gaggttatca
      661 acgccaagat tattaaagcc catacggttg atcaaacctt cggcatctac cagacgaaag
      721 agacgcggct tgtcattacc tggctgtgga cgtggcgtga cggtaccgat ctcgatcgat
      781 ccaaatccca tcgcgcctaa cgcgtcaatg cactccccgt ctttatcaag accggctgcc
      841 agaccaagcg gatttttaaa cgtcaggccc atgcagttaa caggtttcgc aggcactttc
      901 tgccgcacca gtgcttcaaa cggcgttcct gtaatacggc gtaattgctg aaaagtaaac
      961 tcatgagcgc gctctggatc gagctggaaa agggctttac gaacgaaggg gtagtacatg
     1021 aactctcctg gattcccggt gtgcaaaccg ggggcgtatt atgtgcgagt tcggaccaaa
     1081 agggaattga cctgcggcaa aaaaaacgca aacgttttcc gaacctgttt cagggcagat
     1141 tttgctcctt ctttgcactc aacccgccgt aatcatttat cgcccgccag gcgacatgac
     1201 acggtgcgat tgcctgacac cagcttgtct gacgctggct tttaggcggc acaactcccg
     1261 cattacttaa cgctttacca tttacgctca actgaatcag gttaatgtag taaggcgttg
     1321 ggttagtgag ttgtacaccc tgcgaattcc gtgtccagcg taattgttga taagcttcca
     1381 gcgggtcgcc cggcaaacct tcgggtcgcc agaacagttt aaataccgag cgcatcgcca
     1441 cttttacgct ctgattttca actttgccgg atggcacgct ggcaatgctt aactcaaata
     1501 gcgtttcgcg atccacaggc aagatgtcgc tttccgttct cagcaagcgc aacgtgcctg
     1561 tagtaccggg cttcaggaga ataagtggcg gagcggccag taatggtgct ggcactgtcg
     1621 acgcttcccc cccggcccaa cgcgttgggc ggttgatttt actgttaata agccaggatt
     1681 cctgactagt attagtcagt aaaatagata tcgattctct gtctgccgga aagataaatc
     1741 gcgttccgcc gaccaccact cctgcccgca attcacccgc caccagcagc gataaaacgg
     1801 cgaaaatcaa gagcgccaat ctgttccacg tattagtcat aatgaagtac aaatgtcgcg
     1861 ctggcattca ctttacccga ctgcacatcg ctattgacag gtcgcaattg cgcataaaaa
     1921 gttaatggca ccttaccgct atgtaggctg tagtctttac tcgcctggtt aagcggaatc
     1981 aatatcttcg catcatccag aatcgcaatt gccagcccgc tggctgcatc cgtaccactt
     2041 tcaatggaca gcatttcgct ctgcactccg tcgggggttc ctgaaaaggt gacgtcaacc
     2101 gtgctgacat ctgttccgca atcctgcaaa ttaatgatga aagatttctc cgggccataa
     2161 ttcccatgaa aaaaacgtgt cagcgagaca tcgcctaact gcacattgat ttgcttgcta
     2221 tcctgtgcca gcacgcatgt attacggcga aacgatccgc taatgttgac tgtgccatca
     2281 tgggcaaata gctcacagcc ccccgttaat cccagtaaga acagaatcca gattatccgt
     2341 ttcaacatca ctccttcact ggtaaaccag atcaaaatag agaaccgccg tcgcattacc
     2401 gcccgttgct cccgcctttg tggacttata acgaagttga tatttgagtg tgttatcccc
     2461 ggcttttaag ggcgtaagag gctggacctg attgagcggg atttcttgct gactttgcgc
     2521 atcaagaatt tgcacggcaa tccccgttgc cataccaccg cttccgcctg tgtcagttag
     2581 tttgagcagt gtcggcgcaa gggtgtcagc ctcgccgcta aaaaggacgt ccgcgccagt
     2641 aataccagcg gcacaaccgg tgatatcgat attcaaatca gcagcagtgc ttacggtatt
     2701 cgcggcctga aaatcactga tattaaaatc accaatatga atattttgta atgcactttt
     2761 ggtggcgaca tcacagccct gatcgaccac attcccataa atatgcatac taacttgttc
     2821 aggcccagcc atcccgacat ggatgttaag aatgctaaag aacaagataa cgcactgaaa
     2881 tatcgttttt ttcttcatac gttcacgcgt taatcaaaat ttattctcac cgttgccgtg
     2941 gcttcaaatc gcccggtttc tggtggtaca cctgtcgtgc tgactggata gacattcatt
     3001 cgcgcgatac tgtctgcatc atcaggaata gagagcggta atgttccacc agcaggggaa
     3061 ataatgttgt tctggctata ggtcaccacc acgcccacgc caggacgtga cgtcttaatc
     3121 gcctggggat agctggggtc tgtggttgcc gtcagactca ggttgacgtt aactaacccc
     3181 gcgtttcccg tgttacacct gactggaatt gcaagttctg ccgttctggc cccaagaggc
     3241 ttatgcccta caacacgaaa gttagccgca aatacgtcgc ccagatcgac ctcgattgtt
     3301 tctccggcat tgacggaaca tgagccaagt gaattgatag taccagacag gtaatacact
     3361 aaaaccggtg tacctgtagt cgtgcaggaa tcacctgcgg gtatgttgta gcattcaaat
     3421 aatcgcgcca gcgccatagg aggaatgacg accgaaccaa cgaacggctg caatattttc
     3481 agggaaaagc taccgctatt accgctagcc ccctggccac ctaaacgttg accgccggga
     3541 tcgcaaatat gccatcgtgt atctgcccct gtgcccacgt tttcgaaagg aaccgcagta
     3601 ggagaactgc ctttaacctg caataaaact ttggcggcaa gatagtcatt aattttgtac
     3661 cagttgtcgc ctaaatagac taacggtgaa tccgcggcga atgcccatcg gccacttctg
     3721 acattgtctt tatcgcaatc acaggaaaca taataatcat tcgattcgtt ccactgataa
     3781 aatttgtctt taactactcc agccgtattt tcttcttcct ccagggtttt aacgccaaaa
     3841 ttgacgccaa tatacggatt cccctgagtg gtatgacact gcccgctata agcagcagtt
     3901 tgagcgcacc atagcattaa gacggcggca aaaaatagtc gtagtaataa cacgcatttt
     3961 tctccaaaga ttatctgcat atggcatcca ttcgcgtaat cacagagtta atttgttgtt
     4021 cggtaagctg gtaactgata tgacaaattt gatcgggagc ttcgccccag cgtacagtta
     4081 tcgctccctg agcgggcaaa ccggacatat agagcgagcc atcatcatca ataatgccgc
     4141 tattagtctg tatgccgtcc agcgtcgcta tcgcgccaaa acgcagcggt ataccctgct
     4201 ttgatgtttt cattaatacc cttgctcccg catgggcatt gaactccgcc agaaccaacg
     4261 cccctttggt gggaaccacg ttgactactg cattttcaag atccacgtta cgttttaacg
     4321 atgccgcatc aagtgcgatt cgattttccc gataagaagt ggcatagggg acaacggtat
     4381 atccgcgcca gtcagttttc agcccggtgc tgttcgccac acgagtattc tctgcgcctg
     4441 gtgctgcaat cagcacgtta gtttccccca gcgattgccc cagggtaatg ccctgtgaat
     4501 gggcaactaa actgcctgaa agagcgtagt tcagttgttg ttgactgccg ttatcgctgt
     4561 agttgtagcc cactcgggca tctgcaaacg cccctttgta gtccatgctg gcgctaccat
     4621 tagccgtttt cccctcgctg ttatatccct gttgcacgct gtagctcagg ttaccgtcat
     4681 cgagtagtgt ctccgttaaa cccaggtttt gtgtaatgtg cccttcgtta tcgatactgg
     4741 tgttagaggt catccaggcg taattttttt cctggtatag ctatcattac ttctggggag
     4801 caacagattg agcggcaggg aaaaatttag tgcaaacacc tgatcagcat caggttgacc
     4861 acgggactta ctgtagttcc aggaaccccc cagcgagaga tctttaattt gcgtgttgta
     4921 gccaaactgt aatagccgat cctgttgatc ggtatgccag taagtttgct ggctaccact
     4981 taaataaaac gaaccgtact cgcctaattg ctgggagata ttgacctgca gtttgccacg
     5041 tttggtgtaa aacaaattgt aataacgcga ccacatcggc gtatcttcat catcaccgtc
     5101 attaaattcg tagccatcca tatgtttata catggtgtcg gaaagggtgt agaaacccga
     5161 cgtcgaatag cgatagccca tcaattgaaa attagtgccc aattggttga gcgttttggc
     5221 atacagaaaa cgatatgact gacctttatg ttcgctttgg tctgccagcg tactcttcgc
     5281 ctgggtcgca tcgaacgaaa ttgctccgaa atcgccaagg ttaaatccca gaccaaacat
     5341 ggcggcacgg taatattcag catattgtcc accaccatac catgtcgttc cccacggtcc
     5401 gccccactgc aacgtggcct gggcaaattt gctctcctgc tgttcattac tattggtttg
     5461 tatttcgcca gtgtcaccgc gtatttgatt cgcccctgat gctggagtaa tggcacgctg
     5521 gaaaacggta cgctgtagct attgacgctg ccgtccgctt ctttgatttc aactaacaaa
     5581 tcaccgctcg acgacgtgga atagagatca ctaatttcaa aagcaccagg cgatacataa
     5641 gtttggtaaa tggtgtaacc attttgctta atcgttatct gggcattgct tttggcgata
     5701 ccacgcacca caggcgcaaa ccctttcagg ctgtctggca gcatgttgtc atctgacgcc
     5761 agctgcacac cacgaaagct gacactatcg aaaaaatcgc ctgcagtata atcatcacct
     5821 acggtcagtt cgccttttag tggaataacc gcccgctgca aatagctgct gagattcttc
     5881 cattctgcgg tttggccact actgcgactc catgttgaat tgttgcgcaa tctccatggg
     5941 cctaaattaa cgccactgtt taaattcaga aaatagctgt cgccagaatc actgtctgcg
     6001 ctgctatgaa tactgttagc cccgctaaac gaatatccca gtaatagcgc gttgatccct
     6061 tcatcccaac gttcaggggg gacataacca cgggcattac ttttgatggc gatttgcggt
     6121 actgaaattt ccagacggag tttattcaca ttaaacgtta cgctggcatc cggaataatc
     6181 tccgccaatg gcacacacgc ttgtttgtcc agttgtttaa aagccggaaa cgcatcagta
     6241 ttaacaccca ttgcatccag gctttcagtc gtcaaacagg cggcaatccc cttctctggc
     6301 gttatctcaa caaaattgac gttgcgcgta tctacaattg tctgattcag aacaatatcg
     6361 acacgataaa cccctggcgt tattttttgt cctttttcga aacgagacaa atcggccacc
     6421 attgagggat catctttgaa aaaagcagga tcgaaccagg tttcagcttg tgcattgaac
     6481 gctgccgaca caaacaccac cagcccacca ataaacgatg gcactccacc agagcttaac
     6541 aggctgtgtt gtcgatgagt tctatacatc tcgctctctt aactgaagac ggcttacgat
     6601 ttacagttga gttgcggcgt ttccgccccg tagtcattga tggttcgata cgttacgctg
     6661 ctatttttgg ccccctttgg cagagaaaat tggtatttat caaaggggga aatcatttga
     6721 tttttaacta cttttccacc tattttcagg tctgtcaggg taatgaaata aggtgttggg
     6781 ttactgaccg taaccatgtc agctgacgaa gtgcaacgca gtttcgccgg agcctcgcct
     6841 aagggatacg ctaaaccttt tggtcgccag aaaagtttga tgacgctttg tgtggcaatc
     6901 ttcagggtat ttccctcttc ccgcttcgtt gttgaaggga ctgcccgtac gctagtgaag
     6961 aaaagggttt ctctgtcttt cgccaacggc gcaccaatgt acataatgcg taacagattc
     7021 tcattagccg gattcagcac aaatagcggt ggtgtaataa taaaatctgc tgatttttta
     7081 ttatctgggg tcatcaccca tgactgaata agataaggaa catccggact ggtgttacgt
     7141 acacccagtg aaatttgctc ttcaccttct gaataaacaa gacgtgttgc tgcaagtcca
     7201 atcccgcctt tacctgccgc ccatgcggat gagcagctga gtaatattgc cgttaaactt
     7261 acggtcacaa ttccctttgt tatgcaagtt ttcataaata gtcactctta aaaaatatga
     7321 atatcccctc cagaacacta gaggggtact aattaataaa acaattaatg agttctatta
     7381 ataatagtta atatggaaat ttgttaccgc attcacatta ccagaagtcg ctttccccgt
     7441 agcgatataa tcaactttaa aggtcactgt ttgctcagca gaatccgcta actgaatatt
     7501 cgacgtttcg gtaccgggag ttaccgatgt agaaccatct ggcagataca tttgtagagc
     7561 aacattcgtc gctgcatcgg tggtagcctg gttggcaaat gctgtaggtt gcacgccgtc
     7621 agccgtacca ctaaaggtaa acgtcgcatt tttagttaca gtaacatcac agccttctaa
     7681 ggcaattggt acatcagttt taacgccaga gactgctcca ttagtttgca acttggtggc
     7741 gctgacattt ggcaatgtga ctactgaatc tttcgtcgca gctaccaggg tacaagctgg
     7801 agcaataact ttaccattaa atgttacttt gccatccgtg atagcattat catcagctgc
     7861 cataacgctg gacgttgcac ataccacagt tataaacgcc gtcaatacac tttttttcat
     7921 cgtcatcatc ctgatctctt cgtaaatatg ataatgcctt taaatgacat tagacttaat
     7981 aataacaata attgaccgcc atttaaatca agcatactaa atccaataag tgacaaatct
     8041 ttaatttata gatttcatga atatttaaat attaactgaa cgagtaattc attgattcaa
     8101 catctaatga ctatccaaca aataaatatg cgtgatatat aaagcccttt ctttagttta
     8161 ttttcagaaa aagatacaca aaatttatat ttggcatttt cttgtctctc cgacagactg
     8221 accgaattgt tatcaatgtt aacaaaaaaa agaacaattg gttataagga gagagtatgc
     8281 gtgtcatcac cctggcgggt agtcctcgct ttccttctcg ctccagctcc ttgctggaat
     8341 atgcgcggga aaaactaaat ggcctcgatg tagaggttta ttactggaat ctgcaaaact
     8401 tcgccccgga agatctgctt tatgctcgtt tcgatagtcc ggcactcaag accttcaccg
     8461 aacagctgca acaggctgat ggactgatag tcgctacacc tgtgtataaa gccgcctatt
     8521 ccggcgcgct gaaaaccctg ctcgacctgc tgccagagcg cgctttgcaa ggcaaagtgg
     8581 tgctaccgct ggcgacgggc ggtaccgtgg cccatctgct ggcggtcgat tatgccctta
     8641 aaccagtttt aagcgcactg aaagctcagg agatcctgca cggcgtgttt gccgatgact
     8701 cacaagtaat tgattaccat cacagacccc agttcacgcc aaatctgcaa acccgtcttg
     8761 ataccgcgct agaaactttc tggcaggcat tgcaccgccg cgatgttcag gttcctgacc
     8821 ttctgtctct gcgaggtaat gcccatgcgt aacatcatta aactggcgct ggtgggattg
     8881 cttagcgtct ctacgattgc ggttgctgca gaatcctcgc ctgaagcgtt acgtataggc
     8941 tatcagaaag gcagtattgg tatggtactg gcaaaaagcc accagttact ggaaaaacgc
     9001 tatccgcaat caaaaatctc ctgggtggag ttccccgcgg gtccgcaaat gttggaagcg
     9061 ttaaactttg gcagtattga tctcggcagt accggggata ttccgccaat ctttgcccag
     9121 gctgccgggg ctgatttggt atatgtgggc gtcgagccac cgaagcccaa agccgaagtg
     9181 attctggtgg cagaaaacag cccgatcaaa accgtagccg atcttaaagg tcacaaagtt
     9241 gcctttcaga aaggttccag ttcacacaac cttttactgc gtgcactgcg tcaggccgga
     9301 cttaagttta ccgatatcca acccacttac ctgacgcccg ctgatgcccg cgccgcgttc
     9361 cagcaaggta acgttgacgc ctgggctatc tgggatccct actactctgc tgcattatta
     9421 cagggcggcg tgcgggtgct gaaagacggc accgatctca atcaaactgg atcgttttat
     9481 ctggcagctc gtccctatgc agaaaaaaac ggcgctttta ttcagggcgt actcgcaacc
     9541 tttagtgagg ccgatgcgtt aacccgcagc cagcgcgagc aaagcatcgc tttactggca
     9601 aaaacgatgg gcttaccggc accggtgatt gcctcttact tagatcatcg ccctcctacc
     9661 accatcaaac cggttaacgc cgaggttgcc gccttacagc agcaaacggc agatctgttt
     9721 tatgaaaatc gtctggtgcc gaaaaaagtc gatattcgcc agcgcatctg gcagcccact
     9781 caactggaag gaaaacaatt atgagtctga atatgttctg gtttttaccg acccatggtg
     9841 acgggcatta tcttggaacg gaagaaggtt cacgcccggt tgatcacggc tatctgcaac
     9901 aaattgcgca agcggcggat cgtctgggct ataccggtgt gctgatccca acggggcgat
     9961 cgtgtgaaga tgcgtggctg gtggcggcgt cgatgatccc ggtgacgcag cggctgaagt
    10021 ttcttgtcgc cctgcgcccc agcgtaacct cacctaccgt tgccgctcgc caggccgcca
    10081 cgcttgaccg tctctcaaat ggtcgtgcgt tgtttaatct ggtcacaggc agcgatccac
    10141 aagagctggc aggcgacggc gtgttccttg atcatagcga gcgctacgaa gcctcggcgg
    10201 aatttaccca ggtctggcgg cgcttattgc tgggggaaac cgtcaatttc aacggtaaac
    10261 atattcatgt gcgcggagca aaactgctct tcccgccgat tcaacagcct tatcctccgc
    10321 tttactttgg cggatcgtca gacgtcgccc aggagctggc ggcagaacaa gttgatctct
    10381 acctcacctg gggcgaaccg ccagaactag taaaagagaa gatcgaacaa gtgcgggcga
    10441 aagctgccgc gcatggacgc aaaattcgtt tcggtattcg tctgcatgtg attgttcgtg
    10501 aaactaacga cgaagcgtgg caggccgccg agcggttaat ctcgcatctt gatgatgaaa
    10561 ctatcgccaa agcacaggcc gcattcgccc ggacggattc cgtaggacaa cagcgaatgg
    10621 cggcgttaca taacggcaag cgcgacaatc tggagatcag ccccaattta tgggcgggcg
    10681 ttggcttagt gcgcggcggt gccggaacgg cgctggtggg cgatggtcct acggtcgctg
    10741 cgcgaatcaa cgaatacgcc gcgcttggca tcgacagctt tgtgctttcg ggctatccgc
    10801 atctggaaga agcgtatcgg gtcggcgagt tgctgttccc acatctggat gtcgccattc
    10861 cggaaattcc ccagccgcag ccgctgaatc cgcaaggcga agcggtggcg aatgatttta
    10921 tcccccgtaa agtcgcgcaa agctaaggaa gaaataatgg caacgccagt gaagaagtgg
    10981 ttattgcgcg ttgccccctg gtttttaccg gtgggcatcg tggcggtgtg gcaactggcc
    11041 tcctcagttg gctggctgtc gacgcgtatt ttgccctcac cggaaggggt ggtgacggcg
    11101 ttctggacgc tctccgccag cggcgaactg tggcagcatc tggcgatcag ttcctggcgg
    11161 gcgctgattg gcttttcaat tggcggatcg ctggggttga ttttgggact gattagcggg
    11221 ctatctcgct ggggagaacg gctgctggat acctcaattc agatgttgcg caacgtaccg
    11281 catctggcgc tgatcccgct ggtgattttg tggtttggca ttgatgaatc cgcaaaaatc
    11341 tttctggtgg cgctcggcac gctattcccc atttatatca acacctggca tggcatccgt
    11401 aatatcgatc gcggtctggt ggaaatggcg cgtagctatg gattatccgg cataccgctg
    11461 tttatccatg tgatcctgcc tggtgccctg ccctcaatta tggtcggcgt gcgttttgcg
    11521 ttgggcctga tgtggctgac gctgattgtt gccgaaacca tttctgccaa ttcaggcatt
    11581 ggttatctgg cgatgaatgc gcgggagttt ttgcaaacgg acgtggtggt agtcgctatt
    11641 attctttacg ccctgctcgg caaacttgct gacgtcagcg cgcagctgtt agagcgcctc
    11701 tggttgcgct ggaacccggc ttatcatttg aaggaggcca cggtatgaat actgctcgtc
    11761 tgaaccaggg cacaccattg ttgctcaatg cagtaagcaa acattacgcg gaaaatatcg
    11821 tcctgaacca actggattta catattccgg caggtcagtt tgtggcggtg gtgggccgca
    11881 gcggtggtgg caaaagtacc ctgctgcgcc tgctggccgg gctggaaacg ccaaccgcag
    11941 gcgatgtgtt ggcaggcacc acaccgctgg ctgaaattca ggaagacacg cgaatgatgt
    12001 ttcaggatgc gcgtctgcta ccgtggaaat cggtgattga taacgttggg ttaggcctta
    12061 aaggccagtg gcgcgatgcc gcgcgtcggg cgttggctgc ggtaggactg gagaatcgcg
    12121 ccggggaatg gcctgccgca ctttcaggcg ggcagaaaca gcgagtggcg ctggcaagag
    12181 cgttgattca tcgaccgggt ttattgttgc ttgatgaacc gctcggggca ctggacgcct
    12241 taacgcgact cgagatgcag gatttgattg tgtcactttg gcagcagcac ggctttaccg
    12301 tactgttggt gacgcatgat gtgagcgaag cggtagcaat ggctgaccgg gtgttgttaa
    12361 ttgaagaggg aaaaattggt ctggatttga cagtggatat cccccgtccg cgtcgattag
    12421 ggtcggtgcg attagcggag ctggaagcgg aagtattgca acgggtaatg cggcgtgggc
    12481 attctgaaca actgattcgg cgtcatggtt aatatgccgg atgtggcgca caaaaccagc
    12541 attttcaacg gacttgtagg cctgataaga cacgtcaagc atcgcatcag gcagttgggc
    12601 acggctgccg gatgcggcgt gaacgcctta tccggcctac aaaaccagca atttcaacgg
    12661 agttgtaggc ctgataagac gcgtcaagcg tcgcatcagg cattttggct caaaagtatc
    12721 aagccagtgc tttagttatc ttctcgtaaa gatcgccaga gagattttcc agccctttca
    12781 actgttccag cgccgcgcgc attttctcct gacgtttggc atcataacgt ttcaggcgaa
    12841 tcagcggttc aatcagacgt gaagctacct gcgggttacg gctgttgagg tcggtaagca
    12901 tttccaccag gaactggtaa ccgctgccat cttcggcatg gaacgctgcc gggttgctgc
    12961 ctgcaaacgc accaatcaac gaacgaatac ggttcgggtt gctcatggta aatgagcgat
    13021 gctgcaacag accacgcacc gtctccagca cattcgccgc cgggctggtg gcttgcagga
    13081 taaaccattt atccatcacc agaccgtcct gatgccactt atcgtcgtac tcctgcatca
    13141 gcgcgtcacg gcaaggcagc tgtgcagcaa ccgccgcaga aagcgctgcc agcgcatcgg
    13201 tcatattgtt cgcttcgtgg aactgcttgc tcaccagcac gtcagccaga tgcgtttcac
    13261 caaaagcaag gaagcgcagg caggcattac gcagagtgcg tttcgcaata tcttcatgct
    13321 caacacggta ctcgctctgg taattcgcgt tgtaaatagc cagcagctca tccgccagtt
    13381 cagtcgccag agtacgagtg agtgcttcgc gtacttcggc aatagcaatc gggtcgatga
    13441 tatcgaacag ttcagccatt tcattgacag aaggcagcgt caggatttct gccgccagcg
    13501 ccggatcaat cttctcatcg agcagcactg cgcggaaagc gtcagccaca tgcaccggca
    13561 gagacagcgg ctgcccttgc tgatgtcggg cgacgttcag cttgatgtag gttgccagca
    13621 ggctttgtgc cgcatcccag cgggagaaat cattacgcgc atgacgcatc aggaaggtca
    13681 gttgctgatc gctccactta tattccagtt tcactggcgc agagaattcg cacagcagcg
    13741 caggcaccgg ctggaagtag acattatcaa aaacaaaggt ctgttccgcc tgggtgacgt
    13801 tcagcacgga attcaccgga tgaccgcctt tctgcaacga gatcactttg ccttcgttgt
    13861 catacaattc gatggcaaac ggaatatgca gcggctgttt ttctgcctga tccggcgtgg
    13921 ctggcgtgcg ctggctgatg gtcagggtgt actgctcggt ttccggattg tagtcgtctt
    13981 tgacggtcac aatcggcgtg ccggactggc tgtaccaacg gcggaaatgg gagagatcga
    14041 cattcgacgc atcttccatc gcctgcacaa agtcgtcgca ggttgccgcg ctgccatcat
    14101 ggcgctcgaa atagagttgc atccctttct ggaagttttc ttcgcccagc agggtgtgga
    14161 tcatacggat cacttccgcg cccttctcgt aaacggtcag ggtgtagaag ttgttcatct
    14221 caatgaccat gtccgggcgg atcgggtgcg ccatcgggct ggcgtcttcc gcaaactgca
    14281 atccgcgcat ggtgcgcaca ttattgatgc ggttaactgc acgggaacca aggtcagagc
    14341 tgaattcctg atcgcggaag acggttaaac cttctttcag gctgagctgg aaccagtcgc
    14401 ggcaggtgac tcggttaccg gtccagttat ggaaatattc atggccgata acgcgttcaa
    14461 tatcgaggta atctttgtca gtggcggtgt cggtgcgggc cagcacatat ttggagttaa
    14521 agatattcaa acccttattc tccattgcgc ccatattgaa gaaatccacc gcgacgatca
    14581 tatagatgtc gaggtcatac tccaggccga agcgttcttc atcccatttc attgagtttt
    14641 tcagcgaggt catcgcccac ggcgcgcgat caaggttgcc gcgatcgacg tacagctcca
    14701 gcgccacttc gcgaccagaa cgcgtggtaa aggtgtcgcg cagtacatca aagtcgcctg
    14761 ccaccagcgc aaacaggtag cacggtttcg ggaacgggtc ctgccactgt acccaatggc
    14821 gtccgttttc cagttcacct tgcgcaacgc ggttaccgtt ggaaagcagg aagggatatt
    14881 tggttttatc ggcaataatt ttggtggtaa aacgcgccag cacgtccggg cggtcgagat
    14941 aatacgtaat atggcggaaa ccttcggctt cacactgggt gcaaagcgca tcgcctgact
    15001 gataaagccc ttccagcgcc gtattcgccg ccgggcttat ttcattaatg atcttaagcg
    15061 taaaacgctc cggcaaatta ctgatgacca gtgcgccctc ttcttctttc caggcggtcc
    15121 acggctcatc attaatatga acagaaacca gtttgaggtc ttcgccgttg agacgaagcg
    15181 gagcatctga tgcaccatga cggacagcct ggctgaccgc ggtaacgacc gtcttttgcg
    15241 cgtcgaggtc aaaggtcaag tcaatatcag taatctggta atccggcgca cgataatcgt
    15301 gacggtattt ggcttgtggc tgttgagtca taaataacct ttagcatctt ttatagagtc
    15361 tggtgttcag tctattcctg ttgcgtaaat cgcgctatgc agaatcttca tcttttcagg
    15421 tacaaacgcc tttattgcta catttttata acatacaccg cgtaatgcca tcgaccagaa
    15481 aggtggcata tggtgtgatc ggggttcaat aaattgcgaa acaaggtata ctccagcagt
    15541 tcctgaagat gtttattgta ctaaacgctc ctgtacgagg acgctactgc gcacctatga
    15601 cacaattcgc ttctcctgtt ctgcactcgt tgctggatac agatgcttat aagttgcata
    15661 tgcagcaagc cgtgtttcat cactattacg atgtgcatgt cgcggcggag tttcgttgcc
    15721 gaggtgacga tctgctgggt atttatgccg atgctattcg tgaacagatt caggcgatgc
    15781 agcacctgcg cctgcaggat gatgaatatc agtggctctc tgccctgcct ttctttaagg
    15841 ccgactatct taactggtta cgcgagttcc gctttaaccc ggaacaagtc accgtgtcca
    15901 acgataatgg caagctggat attcgcttaa gcggtccgtg gcgtgaagtc atcctctggg
    15961 aagttccttt gctggcggtt atcagtgaaa tggtacatcg ctatcgctca ccgcaggccg
    16021 acgttgcgca agccctcgac acgctggaaa gcaaattagt cgacttctcg gcgttaaccg
    16081 ccggtcttga tatgtcgcgc ttccatctga tggattttgg cacccgtcgc cgtttttctc
    16141 gcgaagtaca agaaaccatc gttaagcgtc tgcaacagga atcctggttc gtgggcacca
    16201 gcaactacga tctggcgcgt cggctttccc tcacgccgat gggaacacag gcacacgaat
    16261 ggttccaggc acatcagcaa atcagcccgg atctagccaa cagtcagcga gctgcacttg
    16321 ctgcctggct ggaagagtat cccgaccaac ttggcattgc attaaccgac tgcatcacta
    16381 tggatgcttt cctgcgtgat ttcggtgtcg agttcgctag tcggtatcag ggcctgcgtc
    16441 atgactctgg cgacccggtt gaatggggtg aaaaagccat tgcacattat gaaaagctgg
    16501 gaattgatcc acagagtaaa acgctggttt tctctgacaa tctggattta cgcaaagcgg
    16561 ttgagctata ccgccacttc tcttcccgcg tgcaattaag ttttggtatt gggactcgcc
    16621 tgacctgcga tatcccccag gtaaaacccc tgaatattgt gattaagttg gtagagtgta
    16681 acggtaaacc ggtggcgaaa ctttctgaca gccctggcaa aactatctgc cacgataaag
    16741 cgtttgttcg ggcgctgcgc aaagcgttcg accttccgca tattaaaaaa gccagttaat
    16801 atcatcaggg agctaatcgg ctcccctttt tttaccttta attccgaaat ctttcgctgc
    16861 atttgcgaat tctgcttgtc tgattgcaga tgccaggtaa cataggtatc cccccattta
    16921 cgggtggaca agtgtttatt ttttccgact attaacagag agaatattat gagcgttgtg
    16981 cctgtagccg acgtactcca gggccgcgta gccgttgaca gcgaagtcac cgtacgcgga
    17041 tgggtacgta cccgccgaga ttcaaaagct ggcatctcct tcctcgccgt ttatgacggt
    17101 tcctgctttg atcctgtaca ggctgtcatc aataattctc tgcccaatta caatgaagac
    17161 gtcctgcgtc tgaccaccgg ctgctcggtc attgtgacgg gtaaagtcgt ggcgtcgccg
    17221 ggccaggggc aacaatttga aattcaggcc agcaaggttg aagttgctgg ttgggttgaa
    17281 gatccagaca cttacccgat ggcggcaaaa cgccacagca ttgagtatct gcgtgaagtc
    17341 gctcacctgc gtccgcgcac aaacctgatt ggtgccgtcg cgcgcgttcg tcatacgctg
    17401 gcgcaggcgc tgcatcgctt ctttaacgag cagggattct tctgggtttc aacgccactg
    17461 attaccgcat ctgataccga aggtgcaggc gaaatgttcc gcgtttctac gctggatctg
    17521 gaaaacctgc cgcgtaacga tcagggcaaa gtggatttcg acaaagactt ctttggtaaa
    17581 gagtctttcc tgaccgtatc tggccagttg aacggcgaaa cctacgcttg cgcattatcc
    17641 aaaatttata ccttcggccc gactttccgt gctgaaaact ccaacaccag ccgtcacctg
    17701 gcggaattct ggatgctgga gccggaagtg gcgtttgcta acctgaacga tatcgcgggt
    17761 ctggctgaag ccatgctgaa atatgtcttc aaagcggttc tcgaagaacg cgcagacgac
    17821 atgaaattct tcgctgaacg cgtagataaa gatgccgttt cacgtctgga acgcttcatt
    17881 gaggccgatt tcgcgcaggt ggattacacc gacgcagtga ccattctcga aaactgcggc
    17941 aggaagtttg aaaacccggt ttactggggc gtcgatctct cttctgagca tgagcgttat
    18001 ctggcggaag aacactttaa agcaccggta gtggttaaaa actatccgaa agatattaaa
    18061 gcgttctata tgcgcctcaa cgaagacggt aaaaccgttg cggctatgga cgttctggct
    18121 ccgggcatcg gtgagatcat tggtggctcc cagcgtgaag aacgtctgga cgttctggac
    18181 gagcgtatgc tggaaatggg cctgaataaa gaagattact ggtggtatcg cgatctgcgt
    18241 cgctacggta ctgttccgca ttcaggtttc ggtcttggtt ttgaacgcct gattgcttac
    18301 gtaactggcg tgcaaaacgt gcgtgatgtg attccgttcc cacgtactcc gcgcaacgcc
    18361 agcttctgat tttcttcctc aaagagccag cgcctgctgg ctctttttta tcctttctgt
    18421 caagttatct gtttgttaag tcaagcaatc tatttgcaac cccgccataa cgatcatcct
    18481 gttacggaat attacattgc aacatttacg cacaaaaaat aacccgcact cttattgcgg
    18541 attagt
//
LOCUS       DAZKIV010000092        18426 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZKIV010000092 DAZKIV010000000
VERSION     DAZKIV010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.092"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            108..182
                     /gene="tnaC"
                     /locus_tag="ACHOT6_003035"
     CDS             108..182
                     /gene="tnaC"
                     /locus_tag="ACHOT6_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFP9948213.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            403..1818
                     /gene="tnaA"
                     /locus_tag="ACHOT6_003036"
     CDS             403..1818
                     /gene="tnaA"
                     /locus_tag="ACHOT6_003036"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFP9948214.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1909..3156
                     /gene="tnaB"
                     /locus_tag="ACHOT6_003037"
     CDS             1909..3156
                     /gene="tnaB"
                     /locus_tag="ACHOT6_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFP9948215.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3288..4463
                     /gene="mdtL"
                     /locus_tag="ACHOT6_003038"
     CDS             3288..4463
                     /gene="mdtL"
                     /locus_tag="ACHOT6_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFP9948216.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIALVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4438..5397
                     /gene="yidZ"
                     /locus_tag="ACHOT6_003039"
     CDS             4438..5397
                     /gene="yidZ"
                     /locus_tag="ACHOT6_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFP9948217.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5554..6303
                     /gene="yieE"
                     /locus_tag="ACHOT6_003040"
     CDS             5554..6303
                     /gene="yieE"
                     /locus_tag="ACHOT6_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFP9948218.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            6325..6891
                     /gene="yieF"
                     /locus_tag="ACHOT6_003041"
                     /gene_synonym="chrR"
     CDS             6325..6891
                     /gene="yieF"
                     /locus_tag="ACHOT6_003041"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFP9948219.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEDGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6945..8282)
                     /gene="adeP"
                     /locus_tag="ACHOT6_003042"
     CDS             complement(6945..8282)
                     /gene="adeP"
                     /locus_tag="ACHOT6_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFP9948220.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            8449..9114
                     /gene="yieH"
                     /locus_tag="ACHOT6_003043"
     CDS             8449..9114
                     /gene="yieH"
                     /locus_tag="ACHOT6_003043"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFP9948221.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9181..9654
                     /gene="cbrB"
                     /locus_tag="ACHOT6_003044"
     CDS             9181..9654
                     /gene="cbrB"
                     /locus_tag="ACHOT6_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HFP9948222.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9703..10290
                     /gene="cbrC"
                     /locus_tag="ACHOT6_003045"
     CDS             9703..10290
                     /gene="cbrC"
                     /locus_tag="ACHOT6_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFP9948223.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10352..11074)
                     /gene="yieK"
                     /locus_tag="ACHOT6_003046"
     CDS             complement(10352..11074)
                     /gene="yieK"
                     /locus_tag="ACHOT6_003046"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFP9948224.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(11089..12258)
                     /gene="yieL"
                     /locus_tag="ACHOT6_003047"
     CDS             complement(11089..12258)
                     /gene="yieL"
                     /locus_tag="ACHOT6_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFP9948225.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGGPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12285..13901)
                     /gene="bglH"
                     /locus_tag="ACHOT6_003048"
     CDS             complement(12285..13901)
                     /gene="bglH"
                     /locus_tag="ACHOT6_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFP9948226.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRFGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13987..15381)
                     /gene="bglB"
                     /locus_tag="ACHOT6_003049"
     CDS             complement(13987..15381)
                     /gene="bglB"
                     /locus_tag="ACHOT6_003049"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFP9948227.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVELNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15400..17277)
                     /gene="bglF"
                     /locus_tag="ACHOT6_003050"
     CDS             complement(15400..17277)
                     /gene="bglF"
                     /locus_tag="ACHOT6_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFP9948228.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17410..18246)
                     /gene="bglG"
                     /locus_tag="ACHOT6_003051"
     CDS             complement(17410..18246)
                     /gene="bglG"
                     /locus_tag="ACHOT6_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFP9948229.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         4666 a         4580 c         4336 g         4844 t
ORIGIN      
        1 ttgctccccg aacgattgtg attcgattca catttaaaca attttagaat agacaaaatt
       61 cttgagtatt atactgtagc ctcgtgtctt tcgaggataa gtgcattatg aatatcttac
      121 atatatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      181 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgaacgatta
      241 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      301 agtaaatgat ggtgcttgta tatatacatg gcgaattaat ggatattgca gatgtaatat
      361 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      421 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      481 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      541 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      601 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      661 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      721 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      781 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      841 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      901 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      961 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
     1021 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1081 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1141 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1201 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1261 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1321 tatggcggcc tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1381 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1441 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1501 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1561 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1621 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1681 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtgaa agagaacgcg
     1741 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1801 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1861 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1921 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1981 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     2041 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2101 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat cggtaacacc
     2161 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2221 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2281 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2341 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2401 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2461 accactgcgg gaacgtctta cttcccgtat atttttatgg ctttgccggt gtgtctggcg
     2521 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2581 aagctaatca aaagcgtggt cttcggttcg ctgctggcgc tggtgattta tctcttctgg
     2641 ctctattgca cgatggggaa tatcccgcga gaaagcttta aggcgataat ctcctcaggc
     2701 ggcaacgttg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2761 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2821 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2881 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2941 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     3001 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3061 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3121 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3181 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc tgatcctcct
     3241 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3301 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3361 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3421 ccgtatatct ggcggggatg gcagctgcga tgttatttgc cggtaaagtg gccgatcgtt
     3481 cagggagaaa gccggtcgcc atacctggcg cggcgctatt tattattgcc ttggtgttct
     3541 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3601 caggctgttg ttacgtagtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3661 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3721 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3781 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3841 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cggtttttcc
     3901 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3961 cgccggtatt gctgatggaa atcatgggtt ttgagcgcgg agaatacgcc accattatgg
     4021 cgttgaccgc tggcgtcagc atgaccgttt cattctccac gccatttgcg ctgggaattt
     4081 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4141 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4201 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4261 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4321 tggcagcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4381 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4441 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4501 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4561 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4621 ctgtcgccca caccgctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4681 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4741 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4801 cgttacccgc aagcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4861 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4921 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4981 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     5041 cgttatccgc atattagcat ttgctgggaa cagagcgaca cctgggcgct ggacaatgtg
     5101 ttacaggagc tggggcgcga acgcacgatt gctatgagcc tgccggaatt cgagcagtca
     5161 ctgtttatgg cagcgcaacc cgacaatctg ctactggcga ccgcgccgcg ctactgtcag
     5221 tactacaatc aactccatca actgccgttg gttgctcttc ctctcccgtt tgacgaaagc
     5281 cagcaaaaaa agctggaagt tccttttacc ctgctgtggc ataaacggaa cagccataat
     5341 ccgaagatcg tctggttacg ggaaaccatt aaaaaccttt acgcgtcgat ggcataaccg
     5401 aatcgtatga aacgcgaccc aatttcacaa taaaatgtaa aaaagttgta ataagcttgt
     5461 ctgaatcgaa cttttagccg ctttagtctg tccatcattc cagtaaatga ttactcttgt
     5521 attcataatg gaccattaag catggagcga aaaatggcga ctcactttgc ccgagggatt
     5581 ttaacggaag gacatctgat ttctgttcgt ctcccctccc agtgtcatca agaagcccga
     5641 aacattcccc ctcatcgtca aagccgtttt ctggcgtcca gaggtttact cgcagaactg
     5701 atgttcatgc tgtatggcat tggcgaattg ccggaaatcg tcaccctgcc gaaaggtaaa
     5761 ccagttttca gtgataaaaa tttgccttcg ttttctattt cctatgccgg gaatatggtt
     5821 ggcgtggcgt taacaaccga aggtgaatgt ggcctcgata tggaactaca gcgtgcgacg
     5881 cgcgggtttc atagcccaca cgcgcccgat aaccacacct tttccagcaa tgaatcgcta
     5941 tggatcagta aacaaaacga tcctaacgaa gcgcgggcgc agctcatcac gctgcgccga
     6001 agcgtgctaa aactaaccgg tgatgttttg aatgacgatc cgcgcgatct gcagctgctg
     6061 cccattgccg gacgcctgaa atgtgctcat gtaaatcatg tagaagcgtt atgcgacgcg
     6121 gaggacgtgc tggtgtggtc cgtggcggtc acgcccgcga ttgaaaagct cagtgtctgg
     6181 gagttagatg gcaaacacag ctggaaaagc ctgccggata ttcacagccg cgccaacaat
     6241 cctaccagcc ggatgatgcg ttttgcccag ctctctaccg tgaagtcttt ttcgccaaat
     6301 tgatagacaa ccacaggagt cattatgtct gaaaaattgc aggtggttac gttactgggg
     6361 agcctgcgca aaggctcatt taatggcatg gttgcacgta ccctgccgaa aattgctccg
     6421 gcgagcatgg aagtcaatgc gttaccttcc attgccgaca ttcccttgta tgacgctgac
     6481 gtacagcagg aagacggttt tcctgcaacg gttgaagctc tggcggaaca gatccgtcag
     6541 gctgacggtg tggtgatcgt cacgccggaa tataactact cggtaccggg tgggctgaaa
     6601 aatgccatcg actggctttc ccgcctgccg gatcaaccgc tggccggtaa accggtgttg
     6661 attcagacca gctcaatggg cgtgattggc ggcgcgcgct gtcagtatca cctgcgccag
     6721 attctggttt tcctcgatgc aatggtgatg aacaagccgg aatttatggg cggcgtgatt
     6781 cagaacaaag ttgatccgca aaccggagaa gtgattgatc agggtactct ggaccacctg
     6841 accgggcaat tgaccgcatt tggtgagttt attcagcgag ttaagatcta aataaaaaac
     6901 ccgccagcaa tcatgcatgg cgggttttta acgcgctatc gattttagtg agcgtcgata
     6961 aagacaatct tcaggataaa cagcagcgca acgatgatta cgcacgggct tagatcacgc
     7021 agacgtccgg taccgatttt catcacgcag taggagataa agcccagcgc aataccttcg
     7081 gtaatcgaaa agctgaacgg catcatcacg gcggtaataa acgccggaac agattcagta
     7141 agatcctgcc agttcacgcg agcaagactt gaggtcatca acacgccaac gtagatcaac
     7201 gcgccagctg cagcgtagcc tggcaccatt cccgccagcg gcgacagaaa gataaccagc
     7261 aggaacagca gaccaacaac cactgccgtc agaccggtac gaccgccaac cgatacgccg
     7321 gaagaggact caatataagc cgtaacggaa gaagtaccga taaacgaacc ggtcacggaa
     7381 gagatactgt cgacatacag cgcctgcttc atgcgcggga atttaccctt ctcatccgcc
     7441 aggcctgctt tatcggtcac gccaatcagc gtaccggagg agtcaaacag gttgaccagc
     7501 atgaaagaga atatcacccc tgccagcccg aggttaaacg acccggctaa atctacatgg
     7561 cccacgacag tcattacgct cggcggcgca gaaacgatgc cattgtagtg gacatcaccc
     7621 agcatccagc ccagcagcgt tgtcaccacg atggaaacca gcaccgccgc gtgaatgttg
     7681 cgcgaggcca gaatagcgat gatgaagaag ccgaggatcc ccagcagcac gctgtgagaa
     7741 gtcaggttac cgatgctgac cagcgtttcc gggttagcga caatcacacc tgcattcttc
     7801 agtcccatca taccaatgaa cagaccgata ccgctggtga tgcccacacg cagacttacc
     7861 ggaatgttag caatcatcca gtaacgaacg cggaaaatcg tcagtaacag cagaccaatc
     7921 gcgccccaga agattgcgcc catcccgact tgccacggca agcccatagc ctgtacaacg
     7981 acaaaagcga agaatgcatt caggcccata gcgggtgcca gtgcaactgg caggttagca
     8041 aacagtccca tcataatact gccgaatgca gcaatcagac aggtagtaac gaagacggcg
     8101 ctggtatcca tgccagcaac gccaagaatt tgcgggttaa caaaaacgat gtaaaccatc
     8161 gtcaggaagg tggtaaaacc ggcgatcact tcggtccgtg ccgtcgtgcc atgttcgcgc
     8221 agtttaaaca cgcgttccag catcccctgg ccagaagcct gggttgtgtg ttgttgactc
     8281 attatctatt tccgaacaag gagggaaaat ccgtcgctat cgtataccaa aatgagacaa
     8341 tatggacggc tgaagataac tttttttcat tgtatttgct tatacggcaa cgattgcgtc
     8401 atgcgaattg gcatgacgaa aacgttaaac taacgataaa ggaaagacat gtcccagata
     8461 gaagcggtat ttttcgactg cgacggtacg ctggtcgaca gtgaagtcat ttgctcccgc
     8521 gcatatgtaa cgatgtttca ggaatttggt attacgctcg atcctgaaga ggtattcaaa
     8581 cgtttcaaag gtgtaaaact gtacgaaatt atcgatattg tttcccttga acatggtgtt
     8641 acgttagcga aaacagaagc tgaacacgtt taccgtgcag aagtcgctcg gctgttcgat
     8701 tcagaactgg aagccatcga aggggctgga gcgctcctgt cagcgatcac tgcgccaatg
     8761 tgtgtggtat ctaacggccc aaataacaaa atgcagcatt ctatgggcaa gctgaatatg
     8821 ttgcactact tcccggataa actgttcagc ggctacgata ttcagcgctg gaagccagac
     8881 ccggcgttaa tgttccatgc ggcaaaagcg atgaatgtaa atgtagaaaa ctgcattctg
     8941 gttgatgact cagttgccgg tgcgcaatct ggtatcgacg caggtatgga agtgttctac
     9001 ttctgcgccg acccgcacaa taagccgatc gttcacccga aagtcaccac ctttacccat
     9061 ctttcgcagt taccggaact gtggaaagcg cgtggttggg atattacggc atagttcttc
     9121 acactccctt cacttacccc gcttaaattg gcgctcaaag gtaagtaaag ggtgtttgat
     9181 atgtctgttt cacgtcgggt aatacatcac ggactttatt ttgcggtttt aggaccgtta
     9241 attggtgttc tgtttcttgt cctctacata ttcttcgcaa aagaaccgct ggttcttttg
     9301 gtaataatac aggtacttcc attgtttctc ttattgtcga ttactacggg agctattcct
     9361 gcattgttaa ccggcgtgat ggtcgcctgt ctgccggaaa agatcgggtc acagaaaaat
     9421 tatcgttgtc tggctggtgg cataggtggc gtcgttatca ccgagatcta ttgtgcagtt
     9481 attgtacata ttaagggcat ggcttcctcg gagttgtttg aaaacattct ttctggtgac
     9541 agtctcgttg tccgcatcat tcctgcattg ctggcaggtg tggtgatgag cagaatcatt
     9601 acccgtctgc ccggattgga tatttcatgt cctgaaacag actctttaag ttaagcggga
     9661 tactttatct ttgggctact caaaagcaga caggatgtta ctatgactca aaatatcagg
     9721 ccgttacccc aattcaaata tcatcccaag ccactggaaa caggcgcatt tgaacaggat
     9781 aaaaccgtag agtgcgattg ctgtgaacaa cagacgtcag tttattactc gggtcccttt
     9841 tattgcgttg atgaagttga acatctctgt ccgtggtgta ttgcggacgg ttctgctgct
     9901 gaaaagtttg caggtagttt tcaggatgat gccagcatag aaggtgttga atttgagtat
     9961 gatgaagagg acgaatttgc cggtattaag aacacatatc ctgatgaaat gctgaaagag
    10021 ttggttgaac gcacgccagg ttatcatgga tggcaacagg aattctggct tgcgcattgt
    10081 ggcgatttct gtgcttttat cggctatgtg ggctggaatg atataaaaga tcgcctcgat
    10141 gaatttgcca accttgaaga agattgtgag aatttcggta ttagaaattc tgatctagct
    10201 aaatgcctgc aaaagggtgg tgattgtcag ggttatctct tccgctgtct ccactgcggc
    10261 aagctgagac tgtggggtga tttttcgtag ttatttaaat aatgagaaca ggccggagcg
    10321 taattcatac atccggcctt atttcttcag cttaaccgag cgctaattct gcggcagcgg
    10381 ctttatcagc gattaccatc agcgaaggat gcagctgcaa cacggaagct ggaacatctt
    10441 cggtaaccgg accttgcagc acatttttca gtgcctgcgc tttacctgcg ccactgacaa
    10501 ttatcagcaa gtttttcgcc gccatgatgc ttttcggccc catcgtcacg tagctgtcag
    10561 gcactaacga aaagtcgccg cccaactcac catgcgccac aatatcaacc atctctccct
    10621 ggatcgggaa ctccacggtt tgctcatgga aatgggtcgt attcggcaaa ttgccacaaa
    10681 aatgaccatc tgcacctaat cccagcacca ccaaatccag gccgccttca cgcgccagtt
    10741 tctgatcatg ctcgcggtag ttatcaatag tgagcttctg gatattctct tctttgatcc
    10801 ccgcaggggt gaaaaacaga ttacgcagat tggtaatcgt tacaccttct ccctctttgc
    10861 cgcgaaaggg aatttcatcg aaattataga aatagcagtt atcgtaccag ggcttacctt
    10921 taaccagggt ggtgaggtat tcatacatgc ctttgggcgt gctaccggcg gtaattgcca
    10981 ggttaacacg acgcgtcttc gacatataac caagcagatg gtgtgccgcg acacggctca
    11041 tttcctggta atcttcggta atgattaatt tcatcttagt gccttatttt atttaaataa
    11101 tttctgtacg aaggctgcat aggcaggccg ccagacatcc atttcatggt tcaggccggg
    11161 atattcctgg taatcaaact taattttttt ctgctcaagc tcagttttca gcccggcgat
    11221 atccttgccg gttacgacat ctttatcacc aacgaccaca gtaaaattac gtagttgctg
    11281 gttaatagct gccggatcgt tcagccgggc cgcgacacct tcatccggta cggttgtcgt
    11341 ggtaacacca ctgaatgtgg ccagccagcc aaagctttcc agatgattca ttccggaaac
    11401 cagcgcctgg tacccgcctt gtgaaagccc tgccagcgcg cgaccatcgg catctttacg
    11461 gacattaaaa cgcttgctaa tcagcgggat aatatcgttc atcagttcgc gatctgccgc
    11521 tttagcattc agcggataaa agactttacg tctttcctga ggcacgaaat cttcgggaat
    11581 aatgcccttc gcatcggttt cggtatccgg gatcaccacc agcatcggtt taattttccc
    11641 ttcagccagc aggttatcca tgatttgcgg gatacgcccc tgatcgatag cggaacgtcc
    11701 ggtatcacca aaaccgtgat agaaatagag cactggcaaa ggctcgccca tgccggtgta
    11761 tcctggcggg gtccagacat acatctgacg ttcagattgc aatgcgttgg agtggtaagt
    11821 tatggcgatc aaatcaccat gcgcaacaga acgcgtatcc agataactgc ctggcaccag
    11881 aatcatactg gagttaacct ggcgctgagg cttagtcatt gcggtgcctg tatcaatgct
    11941 gcgtacacca tcaacattga aaaaatactc gtacaggttg cctttcagga taggtgtgcg
    12001 ccacgaccag actcctgctt cgtctttggt catcgggtga atattgtccg gaaccggacc
    12061 acccaccact acagagacat tttttgcccc aggggcaaag tagcgaaagg tcacgctgtt
    12121 atcggcattg acctgagtca catactgttt caccgggata gtgggtgccg ggctggctgg
    12181 catatccgct gctatggccc actgagcaga aataccgctg gcaatagcca gcgttaaagc
    12241 agcaattttt atattcatcg taatattcct ttcattaagt tccattacca ccagatttca
    12301 gcctgggcac cgacagcaaa ctggtcgtct ttattgtctt cgaaggtgaa gccatccagt
    12361 tcgttttcca gggctttgat atacgtggcg tagaaacgaa tttccggacg cgaggtcaac
    12421 atactggtat tgactttaaa ggtatggaag agcgtggttt tataaccaga ctcattaaat
    12481 ttattactat tcgcatcttt gttttgctgg gtgaaatagc ccagttcaac acctgtctgg
    12541 ttatattggt cccaaatata ggccgggcga acaaccgcac gaatagattc gaaatcagag
    12601 tgtgcgcctg tttcgtagct ataaatatcg ttaccgaagg agtaaacaat cgcgttagcc
    12661 acaataaaat gatcgccgat ataggcttcg ccctgcgagg tcagacgaac tgctgttccg
    12721 ccggtgtgat caccataata acgaccatta aaggtggtaa atggacttgc gccagcataa
    12781 cggccaaagt tactggcgat agagttattc gcaaccagga aggagaattc gttgaagcca
    12841 cctttatcaa atttctgcgt taaagatgtg ccgaacatcc aggtatcttt ccagtcataa
    12901 tacccgttat tatcctgatt atctttttcc gatgcgcttt cgtttgccgt gacataacga
    12961 ccactcacca ttaaggtcgc tttatcccat aacgggatat ctttatagcg taaatcaata
    13021 gtattggtat taatctgctg tttgttttgc aggctgcgat cgtaatcatc gacatcttcg
    13081 cgaaccagcg cgatatcaat tttacccgga ccgactttcc agttttccag acctacaccc
    13141 gctgcggcat cagtacgctg cgttttccag tccagcatct ggatttcaat tttcggcgca
    13201 ccgtgtttac ccacccagaa atcagcctct ggcgcaaagg gcaggaaacc tttggtggta
    13261 acgtacatat cggagaactg cataaagttc tcgccaccgg cgttatcgcc aaaccagccg
    13321 gtagagtact gctgaccaac gttaccatcc atcatcacaa cggcatcaac ccgtttgccg
    13381 ttttcgttgt agacacgttg ttttaactgc aaatcaaacc agccggagta ttcgttacca
    13441 aagcggccca gagaaccaat cgcccatgat ttaggtgaac catgagaggc ggtcccccaa
    13501 ccaaaacggt aatagccgtt gtaactaaag ccaatttcat ctttcacaaa cttactgaaa
    13561 tctttcagag tcatggaaga ataaatcgag cctgtctcac tggcattttt ctcttcattt
    13621 ttgaccagta cagcatcagg ttttgcggca ctactggtcg ggaacggatt ggcggcatac
    13681 ccttgatcat tcgtacttac gctacgattc accgtcgctg gcgtatattt tttcttctct
    13741 tcatctttat actttttgag ttcgctttgc gtttctctta acgctttttc taataactca
    13801 aggcgttgtt ccaccgttaa tgattcagcc aatgattgtg cagaaaaggc taacggtgcc
    13861 attaataaga tggcagaggt aataagattt cgtctaaaca taatatccct ttatggtgca
    13921 aagaaagaat taacgtatcg catccagact gttctgaatg cgacgataat taaggtgctt
    13981 tattggttat ttttttaatg acagcccccg cgttttgatc acttctgcat accatccgaa
    14041 gcttttctta cgcgttcttg tgaggcttcc ttcgccatta tcatcacgat ccacataaat
    14101 aaagccgtag cgcttagaca tttgtgaatg agatgcactg actaaatcaa ttggccccca
    14161 actggtgtac cccataatat ccacaccatc ggcaatcgct tcatttacct gtaccaggtg
    14221 atcgtttaaa taggcaattc gataatcgtc ctgtatcgaa ccatccgctt caacgctgtc
    14281 ttttgcgcct aatccgttct cgacaataaa tagcggtttt tgataacgat cccaaagcgt
    14341 atttaacaaa acccgtaatc caaccggatc aatttgccac ccccactctg aacttttcag
    14401 atgtggattg gggatcatat tcagtatgtt gccctgcgca tttttattaa tgctttcgtc
    14461 gtgggaaaca caaccggtca tgtaataact aaaagagatg aaatctacgg tatgttttaa
    14521 atcttctgcg tcactttcag tcatctcaat ggtgatattg tggtcgcgga agaaacgctg
    14581 catatagccg ggatactgac cacgcgcctg aacatcacca aagaacatcc agcgccggtt
    14641 ctcttccatg gcctgcaaca tatcctgtgg ctggcaggtg agggggtaaa ccagcccacc
    14701 gagaagcata ttgccgattt tcgcttcggg gagcaggcta tgacaggctt taactgcccg
    14761 cgcactggca accagttgat ggtggatagc ctgataaact tccgcctcgc cactctcttc
    14821 tgccagcccc acgcccgtga atggcgcgtg taacgacatg ttgatttcat taaacgtcag
    14881 ccataacgcc actttatgtt ggtagcgagt aaagaccgtg cgggcgtaat gctcgaagtg
    14941 atcgatgacc gctcgattag cccaaccgcc gtagtttttc accagcccat atggcatttc
    15001 gtaatgggat aacgttacca gcggcttgat ccccgcctgc gccatttcat caaacagccg
    15061 atcgtaaaac gctaaccccg cttcattcag ttcgacttcg tcgccctgag ggaaaattcg
    15121 cgcccaggca atggaaatac gcagacaggt gaagcccatc tcggcaaata acgcgatatc
    15181 ttccgggtaa cggtgataaa aatcgatggc gacatctttg atattctctt tccccaggat
    15241 gcgcggttcc atttttccca ttacgccatg aggctgtaaa tctgaggtcg agatcccttt
    15301 gccatcttcc tgccaggcac cttccacctg attggcagct gttgcgccac cccaaagaaa
    15361 tgtttctgga aatgctttca taattaactc cttttatcgt tagcgaatga tggataacag
    15421 cggctcacca gcgcttatct gcgccgtgcc gtggggtaat acgtctgtaa aatcatcgct
    15481 attactgatt aataccggcg tcgtcagatc aaatccggcc tcgcgaatag cagggatatc
    15541 aaaagaaatc agccgatcgc ctgtattgac cttgtcaccc acgttgacgt gagcggaaaa
    15601 gaatttgccg tccagtttta cggtgtcgat accgacatga atcaggatct ccacaccatc
    15661 atctgactca atgccaatag cgtgtaatgt ggcgaacaac gaagcaattc gacccgcaac
    15721 cggagaacgc acttcaccaa ccgagggcag aatggcgata cctttaccca acaggccact
    15781 ggcaaacgtg gtatcagcga cgtgaatgag cggcacaatc tctcccgtca tcggtgaaca
    15841 gataccgccc tgctcaggtg gtgtaataac ctctggtgtt ttctcttgcg gggcaccctg
    15901 cgctggctga cgtttagcgg tgatgaaatg aagcatcacc gtaccgacaa atgcgcaacc
    15961 gatggcaatg acaccgccaa taacgctggc ccagacggtg aaatcaattc ccgttgacgg
    16021 gatggtttgc atgaaggtga aaatacttgg caaaccaaag gagtacactt tcgtttgcgc
    16081 gtagccaatg atggtggccc ccaaagcccc actgatacag gcgataacaa aggggtactt
    16141 acgcggcaga ttaacgccat ataccgctgg ttcggtgata ccaaacaggc ccgtcaacgc
    16201 cgctgatccc gccaccactt ttttctgcgc atcgcgttcg cagaggaaga cgccgagcgc
    16261 cgccccgacc tgcgccataa tggcgggcat taacagcggg atcatggtgt cgtagcccag
    16321 cacggtgaag ttattgatac acagcggcac caggccccag tgcagtccga acatgacgaa
    16381 gatttgccag aagccgccca ttaccgcgcc cgcaaatgca ggaaccgcct gataaagcca
    16441 gagataaccg gcggcaatca gttcgcttat ccaggttgat agcggcccca ccagcagaaa
    16501 ggtgacgggt gtgataacca tcagacatag caatggtgtg aagaaatttt tgattgccga
    16561 cggtaaccac gcattaagtc ggcgttccag aatgctgcac aaccaggcag aaaaaataat
    16621 gggaataacc gatgacgagt aattcaacaa tgtgaccgga atacccagga aatccagccc
    16681 cagctcatcc gctttttgcc cgttctcgaa agcagtcaga attaatggat gcactaacgc
    16741 tccaccaatc accatggcag taaatggatt accgccgaag cgtttccccg cggtgtatcc
    16801 caggattatc gggaagaacc aaaacaaggc atcactggcg ctgaataaaa ttaaataagt
    16861 accactttgt tcggtcgtcc actgaaaagt gagcgccaga gccagcatac ctttcaggat
    16921 cccggttgcc gccatcaaac cgatcagagg cgtaaaaata cctgaaataa cataaacaaa
    16981 gcggtttagc agattacctt tatcatcatt ttccggtgcc tgttgcgctt tttcgtcaag
    17041 gcctgccaca ctgttaaccg ccaggaagac atcggccaca tggttaccta tgaccacctg
    17101 aaactggcca ccgctttcca ccaccataat aataccgggg gtctttttca gtacctctgc
    17161 ttgcgctttg ctttcatcct ttaatttaaa acgtaatcgc gttgcgcaat gcatcagact
    17221 cacaatgtta tctgcgcccc cgactcctgc gactattttt ctggctaact ccgtcataac
    17281 ttgccctcta ccgctttgcg gcaaaactcc aaaaaaaacc tgaaaaaaac ggcctgacgt
    17341 gaatcaagca atttttttca ggttttgccc gcttagtgcg gtaacaatcc tttactcagt
    17401 aataatattt cagtgttctt tgcgcacgcg ctctatattt atggctaaaa acataatctc
    17461 tgcgggtgaa attttacgtt gatactgcaa accaataaaa atggcgatcc gttccgcaca
    17521 ttgccatgct tgcgggtaat tttgttttac tgcttgttgt aatgattcat cactatcgtt
    17581 aattgaagca tgttcaagaa tacgccagga taaaaacttc agatgtgtaa ccagtcgctg
    17641 ataactcaag ctttcttcct ggtaattaag gctgaactga aattttatta attgcagcat
    17701 ttcgcgcatt aactgcgtga cacctgcaac atcctccata tttccgctca tttgggcact
    17761 gaccagatgc atggcaataa agcccacttc atctttcggt aactgcacgc ccaaccgttt
    17821 atcaataatg gttaacgctt cttcccctag ctggaactct ttcgggtaaa gccgctggat
    17881 atcccacagc aacgggttgg gcagcaacac gttttgctga aagcgtttaa tcgcaaactg
    17941 gcaatggtca gttagcgaga tataaatact gtcctgtaat tttcccaagc gctcctgcgc
    18001 taaagagata atacgatcac aggttgccat cacctcaaga ggaatatgac ttaagagttc
    18061 gcttaatcgc ccgttcagtt catgactgct caaggcatac tctttttcta ttccacttga
    18121 gttaattctt tcgccagcgc gtttttgaaa gccaattccg cgccccatga cgactttttc
    18181 ccgctgttga tcatcaataa ccaccacaac attattgttg agaattttgg tgatttgcat
    18241 gttcatagca aggacctttt ttataaacaa aaaaaacccg acttcaccag tattctctgg
    18301 ttatgtcagg ttttgcctgc gaatgcagta acaatccagt catttattaa tggtttttat
    18361 aacgaacatc caggttcgga aattaattta attgcgtgca gagaaaatag caatgcgcta
    18421 ttgata
//
LOCUS       DAZKIV010000093        18184 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZKIV010000093 DAZKIV010000000
VERSION     DAZKIV010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18184
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.093"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(78..653)
                     /locus_tag="ACHOT6_003052"
     CDS             complement(78..653)
                     /locus_tag="ACHOT6_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9948230.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(722..1300)
                     /gene="terD"
                     /locus_tag="ACHOT6_003053"
     CDS             complement(722..1300)
                     /gene="terD"
                     /locus_tag="ACHOT6_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFP9948231.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(1349..2389)
                     /gene="terC"
                     /locus_tag="ACHOT6_003054"
     CDS             complement(1349..2389)
                     /gene="terC"
                     /locus_tag="ACHOT6_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFP9948232.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(2412..2867)
                     /locus_tag="ACHOT6_003055"
     CDS             complement(2412..2867)
                     /locus_tag="ACHOT6_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9948233.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(2890..4047)
                     /locus_tag="ACHOT6_003056"
     CDS             complement(2890..4047)
                     /locus_tag="ACHOT6_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9948234.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(4047..4628)
                     /locus_tag="ACHOT6_003057"
     CDS             complement(4047..4628)
                     /locus_tag="ACHOT6_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFP9948235.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            4951..6009
                     /locus_tag="ACHOT6_003058"
     CDS             4951..6009
                     /locus_tag="ACHOT6_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFP9948236.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            6019..7161
                     /locus_tag="ACHOT6_003059"
     CDS             6019..7161
                     /locus_tag="ACHOT6_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFP9948237.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            7154..7927
                     /locus_tag="ACHOT6_003060"
     CDS             7154..7927
                     /locus_tag="ACHOT6_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948238.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            7929..9008
                     /locus_tag="ACHOT6_003061"
     CDS             7929..9008
                     /locus_tag="ACHOT6_003061"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFP9948239.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            9008..9964
                     /locus_tag="ACHOT6_003062"
     CDS             9008..9964
                     /locus_tag="ACHOT6_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFP9948240.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEAHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            9975..11183
                     /locus_tag="ACHOT6_003063"
     CDS             9975..11183
                     /locus_tag="ACHOT6_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFP9948241.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            11201..11668
                     /gene="terW"
                     /locus_tag="ACHOT6_003064"
     CDS             11201..11668
                     /gene="terW"
                     /locus_tag="ACHOT6_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFP9948242.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            11929..12258
                     /locus_tag="ACHOT6_003065"
     CDS             11929..12258
                     /locus_tag="ACHOT6_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9948243.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            12245..12625
                     /gene="tnpB"
                     /locus_tag="ACHOT6_003066"
     CDS             12245..12625
                     /gene="tnpB"
                     /locus_tag="ACHOT6_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9948244.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            12668..13498
                     /locus_tag="ACHOT6_003067"
                     /pseudo
     CDS             12668..13498
                     /locus_tag="ACHOT6_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(13529..15142)
                     /locus_tag="ACHOT6_003068"
     CDS             complement(13529..15142)
                     /locus_tag="ACHOT6_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFP9948245.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGVLQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(15173..15523)
                     /gene="tnpB"
                     /locus_tag="ACHOT6_003069"
     CDS             complement(15173..15523)
                     /gene="tnpB"
                     /locus_tag="ACHOT6_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9948246.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(15520..15945)
                     /locus_tag="ACHOT6_003070"
     CDS             complement(15520..15945)
                     /locus_tag="ACHOT6_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9948247.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            15999..16750
                     /locus_tag="ACHOT6_003071"
                     /pseudo
     CDS             15999..16750
                     /locus_tag="ACHOT6_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         4424 a         4821 c         4654 g         4285 t
ORIGIN      
        1 ttcccatatt cacccgttga tagcggatcg atacccaaaa aaaaccccgg cattgccggg
       61 gtcagactca gcgtctttta gacattgacg ccgtgctggg aagcaagcgc tgacagacca
      121 ccggcaaaac cctggccaac agctttaaac ttccactcag taccatggcg atacagttca
      181 ccgaagacca ttgcggtttc ggttgaggcg tcttcagaca gatcgaaacg ggcaatttcc
      241 gtcccgttgt cgttgttgta aacgcgcatg aagctgttgc tcaccatgcc gaagttttgt
      301 ttacgcgctt ctgcatcata gatggtaacg gcaaatacca gttttttgat gtctgctgag
      361 actttggtca gatcgatttt gacctgctca tcgtcgccgt cgccttcacc ggtacggttg
      421 tcgccctggt gctctactgc gccatcaggg ctggttttat tattgaagaa aatgaaatgg
      481 gcatctgaca gtactttacc gtcttcacct actgcgaata cggaagcgtc cagatcaaaa
      541 ccctgaccat cggttacacg ggcatcccag cccaagccaa ccatagcgac attcatggtt
      601 ggtgcttctt tggtcagaga tacgttgccg ccttttacga gagaaactgc catttttagc
      661 tcctgcaaac agttgaatga ggctgaatta acagccccag atatgaaaag ttacttttcg
      721 atcaggacgc gttaatgccg tactgagcac atacagatgc cagaccacca gcataaccct
      781 ggcctactgc gcggaatttc cactcaccat tgtggcgata cagctcgccg aacagcatgg
      841 cagtctcagt ggacgcatct tcggtcagat cgtagcgagc gacttcagtc tggttatcgt
      901 cattaaccag acgaataaac gcaccggata cctgaccaaa gctctggcga cgagcctgag
      961 catcgtggat ggtcacaacg aagatgatct tgtcaacttc agacgggacg gcgtccagtt
     1021 taattttcag cgattcatca tcaccatcgc cctcaccggt gcggttatcg ccggtgtgcg
     1081 ttacggaacc gtcggatgac gtcaggttgt tatagaagat gaaatctgaa tcgccgcgca
     1141 ctttgccgtt tgaggccagc aggaatgctg aagcatccag gtcaaagtcc tgaccgtctg
     1201 ttgaacgcgc atcccagcca aggcccacca ggacattttt cattgacgga gctgctttac
     1261 tcagggagac gttcccgcct ttggaaagag aaacactcat aaaataacct cttcgattag
     1321 taattgttca ggttaacact taaggggatt aactcccctt ttcctcagat tcaggtttgc
     1381 ccgggaacat gacgcttgcg agaatgccca gcgccagtac acccagtaca acatacaggc
     1441 tggttgttgc cgcgatgctg taaccatgat gccagatgtg atcggtcgca ttcaggccga
     1501 gttttgccgc gatgaagaac agcagcacga taacggcctt ctccagatga accaggtact
     1561 gtttcagtgc ctcaaggaca aaatacagag tacgcaggcc caggatagca aacatcatgg
     1621 cactatagac gataagcggt tcacgactga cggcaatgat tgccggtacc gagtcaaacg
     1681 cgaacattac gtccgagagt tcaaccacag ccacacacag gaacagcggg gtcgcataca
     1741 gcgccgcttt tttaccacgg ccaatggtga catcgctgtt ttctggtttc gccagttcag
     1801 catccacttc cttctggtta agtaggaagg catgccctct gagcttcggc cagataggga
     1861 agaagcgttt aaccatacgg taagccagat gctgggagta atcctcaatt tcatcatcgt
     1921 catcaccgct tttaagcatc atgaccgccg tccaggcaac gataatagcg aagacaactt
     1981 caacgtacgg ccccagactc agcaggctcg taccgatggc gacaaagatg cccctgaaga
     2041 cgatggcacc aatgatcccc cagtagagaa cacggtggcg ataacgatcc ggaacggcga
     2101 accaggagaa gattgccatc atgacgaaca ggttatcgac cgacagcact ttctccagcg
     2161 cataacccgt gacaaacaga ctggcaacct cagcaccgtg gtggatatag aggaaaccgg
     2221 caaatgccat cgcaaccaca acccagaata cggaccagag cgcagcactc ttcagcgaga
     2281 ttggcttgtc atcacggtgc ataaacaggt caataaagat ggcaccgact gaaagcgcaa
     2341 taaaaacaat gaccgtttca gtcgggaagc cgatgtgtgt ggaaaccata ttttaccctt
     2401 agggatgtgg atcagaggcc aaattcagcc gggtcgaagc ccaacgcgat agcgatctcg
     2461 cgggaggcca gtttctcgtc ctgatcgaag ttaccgtcac ttttcgcaac ggcaatacca
     2521 acacgtaagg ccagctgagc ggcctcaggc tgatctttca gcgccaggat gtatttcatg
     2581 gtttcgccct tgccaatttc aacatcgaaa tcgaagcttg aaaccagttt attgaagaac
     2641 tcaatcacct cattggtatc gaagaccttc agctcttctg aagagcgcag aaagcccatc
     2701 attttctgct tttcttccga acttacgccg tcactcgata cggcaatacg ggcacataca
     2761 gcaacggtgc cctgcatgaa ttttttgttt ttgaaacggc caacctggcg ggtcagttcg
     2821 tcacggcctg agttaatggc acctttaact ttgtcgaaaa agctcatacc tgtttccttt
     2881 gttgctgtgt tatttagaac cggcgctcca gcgaaatccc cagccaaatg cccggtccat
     2941 ttcgtcctgg cctttgaagt actggttaat tcgctcgact ttaattgcgc cgttttcgtt
     3001 tacgagtctg gcaatggcgc acaatgtgcg acggttctca ccttcggtca ggcgggtctc
     3061 aatgggcggt tgatccggca catgaatggt gactacacca tcagtcttgt cccagctggg
     3121 aacaccttca taaataaagg cgtaaatcag cacttcgcgg atatgcttcc attcacgtcc
     3181 attgatatgc agccactcgc cgtctgatac atcacccgtc cggtcatcgc ccttgagctg
     3241 aacatacggt tcatcgcgat aatcaccgaa tgcattaccg agcgcctgaa tgaccgattt
     3301 atacccgtcc tgaagctcaa caaaggcccc cagatccagg tcgattcctt tggagccgaa
     3361 catacctgca aagcctgatt tgctactgcc ccggtgccag ttcaggttga tccggatttc
     3421 accgaagtta tcccgtttgg tcaggctgat tgcaggcttc tcttttgtca gcgatacctt
     3481 gctcaggctg atttttgtct caactggcgg aggggttaca accggaggag ttggggctgc
     3541 gggagccggt tcgtcggcaa tatttacgcc gaaatgctct gccagcggtt taagtccacc
     3601 gttgaacccc tgcgcaacga agcggaattt ccagtcatta ttacgacggt aaaattcccc
     3661 cagaattaag gctgcttcct gacggccgct cagttcgaca ctgccgctaa ccaggccggt
     3721 agccccttgc tccacgtcga tggaaagatt acgaagaccg gacaccgtct gcccaccgtc
     3781 acaggtcacg gtgaacgcaa ttttctggac atcaggtttc agccggttga gcgcgacagt
     3841 aaaggttgag tactggcctt cactgaccag gctgacggta ccatcatcgt tacgtggctg
     3901 accataaaac accatgtccg catcaccctg caccttcccg tcggcataca ggcgaaacgc
     3961 cgaagcatcc acctggccgc ccgaggtgat ccggacccgc aactcctggg ctggaacggg
     4021 ggcattgccc cccggcgtca ggttcattag cgaataaccg ctgaaacgat atcggagtgc
     4081 atatcgtcaa ttgtacggcc gcgacaggca tggccaaggg cggtaaagtc ccagttgccg
     4141 ttattacggc gcagcgaaga aataacgatc ccggtatgtg agccctgctc agtcagcttg
     4201 tagcgagcca gctctttgcc ggtttgatcg acaacgcggc agaaagcgtt ttcgacgtca
     4261 ttgaacgact gaccacggaa gctgtttacc gtaaacgcca ggtattcaac gtttgcaggc
     4321 aggcgggaga ggttgacctt aatcacttca tcgtcaccgt caccctcacc ggtcaggtta
     4381 tcgccactgt gcacaacagc gccgcaggtg gatttcagtt tgcggaacca gatggtgtca
     4441 attgttttac ctgtgctgtc cattaataca cagccagcat cgagatcgat tgagtcgttg
     4501 cctccaaaca atcctccaag aagacctttt ttcttaaccg gatcccaacc gagaccgaaa
     4561 tgaagctggc ttaatgcaga tgattctttg ctgagtgata ccgtctggtt cttaactaat
     4621 gaaaccatgt tttattcctt aaaatggtaa tgaccgagac aatcaaaggt atatttagta
     4681 actgacgaag aaaatcatat catgatgaag ttctctgaca agggaaattt ttgtggtttt
     4741 ctgtaaaaac acaaaaagaa atccttttca gggtgttagt ttttctatgt ttaatttaaa
     4801 tatcaatgtg ttatcaggaa tccaaaaaac actgacgcac tccataacca caggagttat
     4861 taatcatatt atgattgaca tgtagcttag acactcatag actcacttct caatcataaa
     4921 tttgcatggc gttccggata attaaaaaaa atgaacaaga aaatttggtt tatggaaggt
     4981 ttatcctccc agcgagatat tattcagggg gtaaaatcat ttgcacaaaa aaataatttt
     5041 gccattaccg tttttgcctc ccaccgtaac gaaagaaatg aaatcctttc cgttgccgat
     5101 tattctttga ctgaacctga agatcctcaa aaacgtcttc agtttatcca ggaaaccatt
     5161 cagacctacg gcatccacca tattcatact ggccgtaaca gccagtggtt tgaagaacac
     5221 cgttcagcca ttgaaccgac cggtgccacc cttactaccg gtgcaacggg cgtcgactgg
     5281 ttaactctgg ctgacgaaaa agttactttt gctcagttta tggagcaaaa gggtctcccg
     5341 gtcgtaccat cctggcgggt gaatacgctg gcagaattaa agacacacct cgcggccccg
     5401 ccgttcactg acagcccggt atgcgtgaag ccggtgacgg gtatctatgg catgggattc
     5461 tggcgctttg atgacagtgc ttcgcctatg gccgtcttta atcatcccga acatcgtctg
     5521 gtcagtccgc aacagtatat tgcagcagca tcagctgctg agtcgtttaa accccttgtt
     5581 ttgatgccgt acctgccagg cccggaattt tccgtcgata tcctcgcgga taagggcgaa
     5641 atactcgcag ccgtgggacg ccgtaaggaa ggggctatcc agtatctggt aaacgaagga
     5701 agcgcctggg aactggcgtg tgactgcgcc cgtgttatga aggccgacgg gctggtgaat
     5761 gttcagacgc gaaacgatgt gaatggcaac ccggtgctgc ttgaaaccaa catgcgtccg
     5821 tcaggggggg tgggttatac ccttcacagc ggtgtgaacc ttcctgggtt atttgctgcc
     5881 tttaagctcg gtctgatgtc tgaagatatg gtacgccaga gcgctaaaaa caccttttct
     5941 ccggttgcgg tgagatccat tacggatgta attgcatacc cggaatcact ctctaacctt
     6001 ctgaattaag gcgtatttat gtctgatatc ctcgaagata tgatttatcg ccgtaccctc
     6061 tcatgcggta cgattcaggt aacccgtgac cagggtgaag tttcgctcga tgacctgttt
     6121 gacatcgctg aaagacgtaa cccgaaacgc gcctttctgt ttgtcagcaa agtactcggc
     6181 aggcacattc ccgtgccacc ctcagtcatg cggcaggcct acagacagct tgccagccag
     6241 ttcccctcga cactgacagg acccgtactg tttatcggta tggctgaaac cgccgttggg
     6301 cttggggccg gtgtatttga tgaagtgcgc caccagcatc gtgaatctgt ctatctgacc
     6361 tctacccgtc acccggttga tggcacgttg ctttgcgagt tcaaggaaga acacagccac
     6421 gccaccgatc atctgatcta tctgccagat gatgaagaga aaagacgtcg tgttaccaac
     6481 gcacgaacgc tggttttgat tgatgacgag gcaaccaccg gtaatacctt tattaacctg
     6541 ctttctgccc tgcgtaatac cggcaagctt caacatattg aacaggttat cgccgtaacg
     6601 ctgaccgact ggagtggcaa tgccctgtcg gagcgcaccc ccttgcccgt cacttcggtt
     6661 tcgcttgtaa gcggtcagtg gggatggacc ccattacctg atgcacctgt cccggacatg
     6721 ccgaaagtca acgtaacttc acgaggagaa tgggacatcc aggggaaaca gtcatggggc
     6781 cgactgggga tgctcgcacc tgcggccgat ctcggccatg aggtctccgt ccacaagggg
     6841 gaacgtattc tggttctcgg gaccggggaa ttcgtctggg agccgttcct gcttgctgaa
     6901 cggctcgaag ctgccggagc acaggcattt tatggatcga ccacccgctc ccctgtcgcc
     6961 gttggttatg ccattgagtc cgccatttcc tttacggata actacgggct gggcatcccc
     7021 aattttgtct ataacgtcgc ccaccagcag tttgaccgca ttcttgtgtg tactgagaca
     7081 cccgcagaaa gtattgacac gcagcttctt aaagcgctgg ctgaggttgc gcccgtcgtg
     7141 gagattgtta cctatgaata aacccgttgt tctgagcgac cttgacgaca cgctctttca
     7201 gacccgtcgt aaaatggtgg acgagctggc cctggagcca ttccgtaccg gtgccgttga
     7261 tcgcaccctg aatccacgaa gctttatgac ggaagaacag tccatgttgg tggactggct
     7321 cctggagcag gctgaactca tacccgttac cgcacgggga actgaagaaa tcagccgcgt
     7381 acggatccct ttccactcct gggcaatcac aacgcacggg gccgtcattc tcacgcctga
     7441 aggcaaaccc gacgaggagt ggaaggccca tatgctcggc cagctggctc cctaccagga
     7501 aaagctgaca tcgatgcagc gtctgatcac tgaaatgatg gacgcaaaag gaatcaatgc
     7561 ctgggcaagg ctgaacttcg aatacggtga aacggcggtt tatatggtga tgaaacaccg
     7621 cgacagcacc cgccttgacg agctcaatgc cattgcagat gagatagaaa cggtgtttcc
     7681 gaccgagggc ttctacatcc accgcaacag taataacgtg gcctggcttc ccaccccggt
     7741 tgagaaaggg ctggcagtca gctggctgct tgaaaaactt cgggctgaac gaggagtttt
     7801 ccccgtaatt ggtttgggcg acagcctgag cgatcatcgt tttatgaaac tgtgcagctg
     7861 gtttggcatc ccgcgtcaga gtcagtttgc agatgccatt tcacagcgaa tttttggaga
     7921 aaattaaaat gcagccacat gaaacattta ccggctcata ccagcccggt gacgtggaat
     7981 ttctgctaaa gccggttgtc attgagatga cgccggttga gaaaaaagaa gaactgattc
     8041 agtccgggaa gaaacattat tcggacatgc tcagccagga gccagcgcct acacaatggc
     8101 atcttgattt gtttcaccgg gcgctggatc ggggcgcaga gaggcttgca aaagaagtca
     8161 cacagctcgc tattgctctg gccgaacgct tcggtgatga gcccattgta ctggccagtc
     8221 ttgtcagggc tggcgttccg ctcggcgtta tgctgcacca ggccctgcgt gacatgggga
     8281 aaacctcatg gcattacggt atcagcatta tccgggatcg tggaattgac ggtgcagccc
     8341 tcgacgtcat tgaagagcgg catggtacca gcggtattgt ctttgttgac ggatggacag
     8401 gtaaaggcgc aattaccgga gagcttgtac gcgcactgaa agatcgcccg ggctatcctg
     8461 agcagccgcg actggttgtc ctggccgatc cctgtggctg ctcctggctt gcagccagtg
     8521 atgatgactg gctcattcct tttggcatca tgggggcgcc ggtatcaggc ctgatctccc
     8581 ggtcagtatg gtcctctgaa ggattacacg ggtgcatggt ttgcgagcat ctcagtgaat
     8641 tcgaatgcag ccggatgctt gccgataccg tcgctcattt ccgtaagaag ttaacaccgt
     8701 catccctcgc tcccctgagc tggaatacgg aatcagcccg ggtcttatgg cagacaagtc
     8761 gcgacgttat cgcgttcctg gccgatgaat ttaaagtgga cagcgtcaat cgtattaaac
     8821 ccggtattgc tgaagcaacc cgggctgtat tacgtcgggt accggaccat gtatttgtgc
     8881 gttctattga cgacccggac gttgccttgc ttgtagggct tgctcgtgaa aagggaatag
     8941 ttgttacaga aatgggggga accctcggcc agtatcgggc tgtaaccatt atcaagaagg
     9001 tactctgatg aaaaatcgtc tttctccctg gaatctggga gccacgctat acatgcctgc
     9061 aacacgggaa gatattgctg atgccgtcct gcacgggaaa atcccgggct tgcgctctct
     9121 ggtgatttgc ctggaggatg ctgtcagtga agcagacatc cccgtcgccc tcaaaaatct
     9181 ggagcacctg ctgcacgagc tcagtaacag catgagtagc ctgggtaaaa atgactggcc
     9241 tctggtgttc attcgccccc ggcatgccga aatgggcaga tggttaaaag cgcattatga
     9301 tctttccgct gtcgacgggt ttgtgctgcc taaatttacc ctcagttcgt tagctgaatg
     9361 gtgggatatc atggtcggga ctcacctgtg catgatgcca acgctggaaa cagaagatgt
     9421 ctttgacgtg gttcagatgc gcgagctggc tacccgtctg gaggcacatc cctgccacga
     9481 ccgcattatt gcgcttcgaa tcggcggcaa cgatcttatg aacgttgtgt cgcttcgccg
     9541 tccccgggac ctgacgctgt atgacagccc gatgggctac gtcatcaaaa tgctcgtttc
     9601 ggtcttcggc ccgcgtgatt ttgccctgac cgcgcctgta tgtgagcata ttgacgatca
     9661 tgccgttatg gccagagagc tggctcttga tatggcacac gggcttgttg ggaaaacggc
     9721 cattcacccg ggtcagatag aggtcattca aaacgcgctg atggtcactc agggtgagca
     9781 ttctgacgct ctgcggatcc tgaactctac ccaggccgtg tttaagtcgc agggagcaat
     9841 gtgtgaaccc gccacacatc gccgctgggc ggctggcatt ctggacaggg ctcgttttta
     9901 tgggttacag aacgagcaaa gcgctgatgg aatcagatta cttaccgtga cccagcatca
     9961 ttaaggaaat cataatgggg ttcaggttca gaaagtcgat caacattatt cctggcgttc
    10021 gcctcaacct gagtaacggt gcaccgagcc tgagtgtcgg gccgagaggt gcttccgttt
    10081 cttttggtag ccgggggacc tatgccaatc tgggcttgcc cggtaccggg ctgagttacc
    10141 gtacccggct tgaccgggcc gcgcgttcca gaggtgaaaa ccggacggca accgacccgg
    10201 ggctcagaca ggcgcttgag cagaaagccg ctgaactcat gtcagcggta accgcaatcc
    10261 gtaatattca cgagctgacg ccggatccaa aaacaggcat cagctgggca gagctggaag
    10321 cagtatacct gcataacaga acgtcgcctt ttcaggttcc ggcaccggtg cgtccagaaa
    10381 agccagacta ccttgtattg ccggaaaagc ctgccgagag cgaaggcatt agttttttgg
    10441 gtaaatggtt tgaatcggaa tcagctaaag ctgagcgcca cgccgaaaat cttcgccggt
    10501 ggcagcagga gctgattgat gtggagcgtg agaataccct tcgacagcac cggtaccagc
    10561 aacaacggac ggcctgggcc gaacagtatg caaactggaa gtttgaagct gaagaacatg
    10621 aaaaacggct cgccacggct caggcagatg cccggcagca gttccggaca gacgccgcgt
    10681 ttttcgaatc atacctggcg ggtgtgctgg cagaaactga atggccgcgt gaaacgcttg
    10741 ttgcatttga agtaaagccg gagctatcag cagtcctgct ggacgttgat ttagctgaga
    10801 ttgaagattt ccctgataag atttacggcg ttaatgcccg gggaacggag ctgacggaaa
    10861 aagccatgac gcaaaaagcc gtacgcgaaa actatgccca ccacgtccat ggctgcttgt
    10921 tccgcctggt cggtatcgtt ttacatacgc tacctttcga caacgtgatt gtgtcaggct
    10981 ttacgcaacg ggtcagtaag cggaccggct atctggagga tgagtatatc ctgtcctgca
    11041 aatgcactcg cagccagatg tcgtcagtaa attttgcagg cataaaacac attgatccgg
    11101 ttgaagcgtt aggcgatcaa ccggttattc gaaagatgag cagtaccttc atttttcagc
    11161 ctattgaacc actaaccctt taagcaggct aaatcttacg atgcaattaa ataccagaca
    11221 ggcccggatt tttaagcttg ccaacttact gggcaccggc aagcccgttt ccgctgccga
    11281 cattataacc agtctggaat gctctgagcc gacattaacc cgggccctca aagagctacg
    11341 cgagtcatat tctgcggaga tcaaatacag caaggcaggt cattcctatc atctcgttaa
    11401 tccgggccag ttggataaaa agaccctccg ccggatgaat gaagcgctcg cacagaatgc
    11461 tgaactgaaa accggtgagt ccacagggaa ggtcgtactc gataaggata aaaaaacagc
    11521 cgtgtcatta tcgctacgca tgcggattct aagaaaaatt gaccgtctgg ctgcgctgag
    11581 tgactcgacc cgaagtgaag cggtagagaa gctggctctg cactccgttg atgaactgat
    11641 aaaagaatac agcgctaaaa agtcctgacg ctaaatctca gaccggggtg aaaactccgg
    11701 ttttgtcata tttcacctct gtattggcgt gatgcaaaat ggcatctgaa tcgatcatgg
    11761 tttggcatcc atttccgatc acagtttggc atcacaatcg atcacgattt ggcatgcttc
    11821 cgctcattga ttggcatcct ggtgtcgtta cggggaagtc atcttcacag ggatcttcag
    11881 tcagttagca ttaccgacat ccattcacca acttaccgga gataatcaat gtcaaaaccc
    11941 cgatggacgc ttgatcagaa aaaacatcac gttgccgcat ggcgcgccag cggccttacc
    12001 cgtgaacagt actgtgaact ttacgacatt ccgtttaaat ccctgcgcca gtggccgcag
    12061 gatgtcgcaa aagccgaaaa acgagccaga gcaccagaaa ttattcctgt ttcagtatct
    12121 ggctcttccg gaatgactga tggcagacca ttgtcggatg agcctgtcac tctcttcctg
    12181 cccggtggta tccggatgtg ctgccagcct tctcagttaa ccgatgtttt ccgggctctt
    12241 cgccatgctg acgcctgata acgtattcct ggtcgtaaaa cccgtcgata tgcgccgggg
    12301 tatcgacaca ctgacacagt acgtccagaa cgaacttaac gctgcatggc atgacggtgc
    12361 tgcttttgtc ttcaccaata aagtccgctc ccgcataaaa gttctccggt gggacaaaca
    12421 tggcgtatgg ctgtgtaccc gacgactgca ccggggaagc ttccgctggc cccggaaagg
    12481 tgatgccaca tggcatctca cacaggatga gtttcactgg ctggtctttg gggttgactg
    12541 gcaacaggtg aaagggcatg acctggcaaa atgggtatac caggaagaac ccgaagatgc
    12601 tcagagcaac gcaataactc aatgaaatat aatgaatatc catgcgtgat gtcatataat
    12661 ccaccgcatg gatatctcgc ttctcagtac caccagtgac cctgaacagc ttcgcgctct
    12721 ggcaattgcc atggtgcaaa aagtcatggc tgagaatgca gaacttcaga accgtatccg
    12781 catcctggaa gagcagatga agctcgcacg ccagcagcgt ttcggtaaaa aatgcgaatc
    12841 gctggccggg atgcagcgtt ccctgttcga agaggatgtt gacgcggata tcgcagaaat
    12901 ctcggcgcat ctggacaaac tgcttcctca gaccggggac gaagaaaaaa caacaacacg
    12961 tcctgttcgt aagcccttgc cttcacctct tccccgtgct gaaaaggtta tcccgccagc
    13021 tgaggaacgt tgtcctgact gtgatgcccc cctgcacttc attcgcgatg aagtcagcga
    13081 gaagctggaa tatattccgg cacaggtcgt ggtcaaccgc tatatccgcc cgcagtacag
    13141 ctgtccctgc tgtgagaaag tgttcagcgg aaaaatgccg gcacatatcc ttccgaaaag
    13201 tgctgttgag ccatcggtca tcgcacaggt tgtcatcagc aaatataccg accatctgcc
    13261 cctgtatcgc cagcagcaca ttttctcccg aatgggggtg gagctccccg tcagtacaat
    13321 ggcggatatg gtcggtgttg ctggcgctgc actggccccg ctggcaaaat tgttacgcca
    13381 cgagcttctg acacgggatg ttattcatgc agatgaaacg tccctgaggc tccttgatac
    13441 ccggaaaggc ggtaaatcct gctccggctg gctgtgggct tacgtcagcg gagaaagagt
    13501 aagcgtcaac ggagcaccgt attgacgctt atttattggt gagtactacg ttccatggca
    13561 ggagttcatc aactcggttg gaaggccatt ctggcagtac gctcaggata tggcgcagat
    13621 acgcttccgg atcgataccg ttcagacggc aggtgccgat cagcccgtac aacagtgctc
    13681 cacgctcgcc gccgtgatcg ctgccaaaga acataaagtt tttctttccg agacagactg
    13741 cacgaagcgc tctttccgca gcattattat ccgcctccgc cagaccgtca tcactgtaat
    13801 aacagagggc atcccactga ttcagtacat agctgcacgc ttcgcccagt ctggattttt
    13861 tcgacagcgt accattcttc tccaccatcc attcatgcag tgacgtcagt aacactttgc
    13921 ttcgctgctg cctgacggca agacgctctg actccggtaa tccccgtatt tcatcctcga
    13981 tggcgtacag ttcactgatt cgcttcaggg cttcttctgc cgtcgcactt ttgctgctga
    14041 tgtatacatc gtggattttt cgccgggcat gggcccagca cgcaacttct gtcagtgcac
    14101 caccttcacg ttctgcactg aacagccgat cgtaaccgct gaacgcatcc gcctgcagga
    14161 caccccggaa gggacgaagg tgttgtaccg gatgttttcc ctgcctgtct ggtgagtagg
    14221 cgaaccagac cgccggtggc tctgatgagc ccgcattccg gtcatcccgg acatacgtcc
    14281 agatgcgtcc tgtttttgcc ttttttctgc ccggtgccag cacttttact ggtgtgtcgt
    14341 cagtgtgaac cttgcgggtg ttcatcacgt aacggtacag ggtatcattc agcagagtca
    14401 ttaactgaca acatgcgtca acccagttgg agagtaatgc ccggctcagt tcgatgccct
    14461 ggcgggcaaa gatttcactc tgacgataca gtggcaggtg ttcgcagtat tttcccgtta
    14521 acacgcgggc aagtaatccg gagcccgcga tgccgcgctc tatcgggcgg gacggcgctg
    14581 gcgcttcaac tatacagtca cattttgtac aggctttttt tacccgaaca gtgcggatca
    14641 ctttcagggc gctactcacc agttccagct gctcagcact gacttccccc agataatcca
    14701 gctcactgcc acactccggg caacaacttt cttcaggctc cagacggtgt atttcacggg
    14761 gaagatgtgc cggtaacgga cgacgatgac gtgactgtcg caactggcgg ggaacctgcg
    14821 ggtcatcctc acgcccactg taacgatcgc tttcctgttc gcgttgtttc agttgggcct
    14881 cagcctgttc aacctcacgc tgcagttttt cagaacgggt accgaacaac atccggcgca
    14941 gtttttctat ctgggccctc agatgttcta tttcccgctc ctcctcttcg atcttttcct
    15001 cggcacgtgc cagtgcagag cgcaggaagg cctccgtctc ttcaaccaga ctcagttgct
    15061 gatctttctg acggagggct tcagcctgct cagagagcag cctttccagc tcagtgatac
    15121 gaatgaggta tttccgactc atgaccgttt ttataatccg gccatgacat ttttacaaca
    15181 ttgtcagtgc attaaggcgg gatgttttgg gctgacgcca gtccagttta tcgaggagca
    15241 ttgccagctg cgagcgggta atggatacct taccgtcacg caccgcaggc cagataaact
    15301 ggccttcctc cagacgtttg gtgaacaggc acagaccatc agcatcagcc cacagaattt
    15361 taatcgtgtc accccgtcgg ccgcgaaaga taaacaggtg accggagaag gggttctcat
    15421 ccagcacatg ttgtacctgt tctcccagac cgttgaagga tttacgcata tcagtaacgc
    15481 cggcaaccag ccagattcga gtgcctgatg ggagcgagat catcgtcccc tcccggtcag
    15541 ttcacggatc aacaccgtga gcagctctgg tgaaggattt tccagcgtca tgttaccgtg
    15601 gcggaactca actttacagg aactggcact gactgtgctt tgtgaaggag tggataaaag
    15661 cggagtaaga gccgccatag gctctttctg ctcatcaggc gttatctcaa caggtaataa
    15721 ttcaacgcca gcgccagaag aggttgttac cggaagacgc cgcgatatac gcccttcgtt
    15781 ctgccagagc ctgagccatt tgaacaggag gttatcattg atatcgtgtt cccgggcaat
    15841 acgggcaaca gaggctcctg gttgtgaagc cagtttaacc atttgaagtt taaactcatt
    15901 tgaaaatgat ctgcggggtt ctgaggataa tattttctgt tccataacag gtgtccacta
    15961 gttgaaaagg tgggcaccta cgttaccaat actggcttaa tggctacata cggcggtcag
    16021 tttacgctta cgagaaagaa gcggtccgcc ggtcgtctgt tttgactcgc agaccggacg
    16081 ggccctcaga tatcctgagg cctggctgca gggctggtgt ggcggtacgc tggtgagtga
    16141 cggatacagc gtgtacaaat ctctggcgga taatgacccc ggtattacat ctgcctgttg
    16201 ctggagccac gcaaggcgcg gtttcgcaaa tctgtataag gccagcaggg agccccgtgc
    16261 agccatggcg ctgagaaaaa tcgcgggtct ttatcgcatt gagaagttca tccgtgaacg
    16321 tcctgtggaa aaaatacggc aatggcgaca acggtattcc cggccgatag tgaatgacct
    16381 gtttgcctgg cctgaagagc aggagccgtg ctgtccgccc gacggaccac taaacaaagc
    16441 gattaattat atcctgaacc ggcgcgatga actgagctgc ttcctgagtg acggagccgt
    16501 gcccctggac aacaatatct gcgagcgtgc tatccgcccg gtggtcatgg ggcggaaagc
    16561 atggttattc gccggttcac tgatggccgg gaaccgcgcg gcacagataa tgagccttct
    16621 ggaaacggca aaacgtaatg gtctggagcc gcatgcctgg ctgacagacg ttctgacgcg
    16681 tctaccggag tggccggaag accgactgga agagttgctg ccccttgagg gatttacctt
    16741 ctccgggtga gtgatacctg ccatcaggtg ttcgtgcagc gggccataac ccgcagtcgg
    16801 gagttgaacg cctgacgaca ggaaatgagc cagagcagcg accacgcggg ctgacagtga
    16861 gtcagaacct gaatcagccc ggagccattc ccggggctgt acttcatcac ctgccatcag
    16921 tacccgtgtc agtacttcgc gacctgacgc aattacccga ctcagttctt cagaacccgc
    16981 ctccgatacc agcctggcgg tcgcccctgt gcaacaaaat aaatcaacca tcgtcagacg
    17041 gtgatcaatc gtatgcgctg ccagtggctc gcctgaaaaa tgcgtcaata tgagttcgcc
    17101 ggaacaatac ctcagttctt cagaacccgc ctccgatacc agcctggcgg tcgccccggc
    17161 gcaacagaat aaatcaacca tcgtcagact gcgatcattc gtatgcatcg ctgatgatgc
    17221 gcccgaaaaa cgcgtcaata tgagttcgcc ggaatattac tgacgacacg gtttgtagtt
    17281 acgttccttc ggtgagtgag tacctgtgcg tcagggtaac gcaatcagtt caggtttagc
    17341 ggggtgtcaa gtcggtttgg ctaaccgacc gcagaattaa ctgtaccttt aaaggtcgcc
    17401 cccgatatat ctgcctgtca gacatatcgc cgattcggta tctcactaca taaaactcct
    17461 taaaaaacaa tgcgattctg ttggtacgaa gctttcagta agtcagtccg gagaagcgcc
    17521 agtacgatac agaatgaggt tgaaaaaaac gttcacgtct ggtcacttct tcaaatgtta
    17581 aaaaacatct cgtaaagagc cagtttatgc gggttcgttg agtagacgtg cgagcgtcac
    17641 tttgtcagct ttaacgctga cgaaagtgac gcccgcacag cttcaaactc cacatgcaca
    17701 cgcaagcgac caaatttcaa atggtcttat gccgtgcgca cagtcataag aagcgcatgc
    17761 ggaaagtcag aaaatatctt aagacgcaga ttttaaaggc gtttttatgc cagtctgccg
    17821 ggaatacaca gtagtccggt tgtccggaag caccacggta atccgaagga aaaatcaacg
    17881 tacctcaatc accgtagtga tatccgttcc cagaggctaa acggatgtac caccataaag
    17941 gcatcctctt tcaggctacg aggatattgc aatcacccga aggcgtttct ctcagattgc
    18001 tgaaacattg gctggcagtc acccgaaggc gtccctctca gactgctgga acattggctg
    18061 gtcgcaaaag cgacagttaa tgcaaataca ggttaacaaa aagggaaatc tgccgtcaac
    18121 ccaaccatat gaacatgttg atttcattat gaatgtgatg ttctaatata aattaatcag
    18181 taac
//
LOCUS       DAZKIV010000094        17913 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZKIV010000094 DAZKIV010000000
VERSION     DAZKIV010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.094"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(27..1055)
                     /gene="epmB"
                     /locus_tag="ACHOT6_003072"
     CDS             complement(27..1055)
                     /gene="epmB"
                     /locus_tag="ACHOT6_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9948248.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            1097..1663
                     /gene="efp"
                     /locus_tag="ACHOT6_003073"
     CDS             1097..1663
                     /gene="efp"
                     /locus_tag="ACHOT6_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9948249.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            1715..1840
                     /gene="ecnA"
                     /locus_tag="ACHOT6_003074"
     CDS             1715..1840
                     /gene="ecnA"
                     /locus_tag="ACHOT6_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9948250.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            1951..2097
                     /gene="ecnB"
                     /locus_tag="ACHOT6_003075"
     CDS             1951..2097
                     /gene="ecnB"
                     /locus_tag="ACHOT6_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9948251.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            2279..2596
                     /gene="sugE"
                     /locus_tag="ACHOT6_003076"
     CDS             2279..2596
                     /gene="sugE"
                     /locus_tag="ACHOT6_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9948252.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2593..3126)
                     /gene="blc"
                     /locus_tag="ACHOT6_003077"
     CDS             complement(2593..3126)
                     /gene="blc"
                     /locus_tag="ACHOT6_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9948253.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(3215..4348)
                     /locus_tag="ACHOT6_003078"
     CDS             complement(3215..4348)
                     /locus_tag="ACHOT6_003078"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9948254.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            complement(4411..4770)
                     /gene="frdD"
                     /locus_tag="ACHOT6_003079"
     CDS             complement(4411..4770)
                     /gene="frdD"
                     /locus_tag="ACHOT6_003079"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9948255.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            complement(4781..5176)
                     /gene="frdC"
                     /locus_tag="ACHOT6_003080"
     CDS             complement(4781..5176)
                     /gene="frdC"
                     /locus_tag="ACHOT6_003080"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9948256.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            complement(5187..5921)
                     /gene="frdB"
                     /locus_tag="ACHOT6_003081"
     CDS             complement(5187..5921)
                     /gene="frdB"
                     /locus_tag="ACHOT6_003081"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9948257.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            complement(5914..7722)
                     /gene="frdA"
                     /locus_tag="ACHOT6_003082"
     CDS             complement(5914..7722)
                     /gene="frdA"
                     /locus_tag="ACHOT6_003082"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFP9948258.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            8047..9024
                     /gene="epmA"
                     /locus_tag="ACHOT6_003083"
     CDS             8047..9024
                     /gene="epmA"
                     /locus_tag="ACHOT6_003083"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFP9948259.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9243..10745
                     /gene="yjeM"
                     /locus_tag="ACHOT6_003084"
     CDS             9243..10745
                     /gene="yjeM"
                     /locus_tag="ACHOT6_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFP9948260.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            10797..11111
                     /gene="yjeN"
                     /locus_tag="ACHOT6_003085"
     CDS             10797..11111
                     /gene="yjeN"
                     /locus_tag="ACHOT6_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjeN"
                     /protein_id="HFP9948261.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            11108..11422
                     /locus_tag="ACHOT6_003086"
     CDS             11108..11422
                     /locus_tag="ACHOT6_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HFP9948262.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(11451..14774)
                     /gene="mscM"
                     /locus_tag="ACHOT6_003087"
     CDS             complement(11451..14774)
                     /gene="mscM"
                     /locus_tag="ACHOT6_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFP9948263.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(14796..15764)
                     /gene="asd"
                     /locus_tag="ACHOT6_003088"
     CDS             complement(14796..15764)
                     /gene="asd"
                     /locus_tag="ACHOT6_003088"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFP9948264.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(15861..16913)
                     /gene="rsgA"
                     /locus_tag="ACHOT6_003089"
     CDS             complement(15861..16913)
                     /gene="rsgA"
                     /locus_tag="ACHOT6_003089"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFP9948265.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            17008..17553
                     /gene="orn"
                     /locus_tag="ACHOT6_003090"
     CDS             17008..17553
                     /gene="orn"
                     /locus_tag="ACHOT6_003090"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFP9948266.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            17764..17839
                     /locus_tag="ACHOT6_003091"
     tRNA            17764..17839
                     /locus_tag="ACHOT6_003091"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17797..17799,aa:Gly,seq:gcc)
BASE COUNT         4096 a         4621 c         4741 g         4455 t
ORIGIN      
        1 aatgtttaac ttccctgttt aatcagttac tgctggcgta gctggagatc cagcggcgtt
       61 ttgctgggtt cgccgccaat ttctcgcgcc agtttcggca ccagatatcc cgacaccaat
      121 gtcagcaact cacgcataat ctgccgtgct tcgtcatcac tcaccataaa atgcgccgcg
      181 ccctgtactt tatctagcac atgcaggtaa tacggcatca cgccggcatc gaacaacgca
      241 ttactcaggt ttgccaacgt ttgtgcgtta tcgttcacac cacgtaacag aacgctctgg
      301 ttaagcaggg tgacaccgac acggcgtaat ttagccatcg cctgacggaa cgtttcatct
      361 acctcattgg catgattgat gtgattcacc agcaagattt gcagcgtaga acgggcaaag
      421 cgttcaacca gcgcctcggt gatacgtgcc gggatcacaa tcggcagacg gctgtgaatc
      481 cgcagacgtt tgatatgcgg gatggcttcc agttgtgtga gcaaccagtc cagctcgtga
      541 tctttcgcca tcagcggatc gccgccggag aaaatcatct cgtccagttc cggatgcgca
      601 gcaacatact caagtgcagt ctgccagtta cgcttgttgc cctgattttc ggcatagggg
      661 aagtggcgac ggaagcaata gcggcaattt accgcgcagc cgcctttgac cagcaaaagc
      721 gcccggttgt ggtatttatg caacaaacca ggcactacgc tgtgctgttc ttccagcggg
      781 tcggtggaga atccggacgc gacgacaaac tcatcttgcg aggtaagtac ctgacgcaaa
      841 agaggatcgt ccggattgcc tttctccatg cgatcgataa atgagcgggg cacacgcagg
      901 gcaaacagct ttttggcgct gcgtccggcc aacagttttt cgtccgcgtc tatattcaaa
      961 agacgcagaa gttcatcagg atcggtcaca acatcggcaa gttgcgttaa ccaatcttct
     1021 ctggatgggg tatttagggt tacaatatgc gccattttgt ggcttagcta ccaattaaca
     1081 aatttcagag ggccttatgg caacgtacta tagcaacgat tttcgtgctg gtcttaaaat
     1141 catgttagac ggcgaacctt acgcggttga agcgagtgaa ttcgtaaaac cgggtaaagg
     1201 ccaggcattt gctcgcgtta aactgcgtcg tctgctgacc ggtactcgcg tagaaaaaac
     1261 cttcaaatct actgattccg ctgaaggcgc tgatgttgtc gatatgaacc tgacttacct
     1321 gtacaacgac ggtgagttct ggcacttcat gaacaacgaa actttcgagc agctgtctgc
     1381 tgatgcaaaa gcaattggcg acaacgctaa atggctgctg gatcaggcag agtgtatcgt
     1441 aactctgtgg aatggtcagc cgatctccgt tactccgccg aacttcgttg aactggaaat
     1501 cgttgatacc gatccgggcc tgaaaggtga taccgcaggt actggcggca aaccggctac
     1561 cctgtctact ggcgctgtgg ttaaggttcc gctgttcgta caaatcggcg aagtcatcaa
     1621 agtggatacc cgctctggtg aatacgtctc tcgcgtgaag taatgcggtt gtggtgcggc
     1681 ctgcaggctg caccatcact tattcaggtc agagatgatg aaacgcctta tcgttcttgt
     1741 tttgcttgcc agcacgctgc tcacgggctg taacaccgct cgcggtttcg gcgaagacat
     1801 caaacatctc ggcaactcca tctctcgcgc tgccagctaa tttttcttct cttccgaaaa
     1861 atcatcagat ttccgtcatt tttggcgatg ttgtctatta ttaatttgct ataggcaaac
     1921 ataaataaca ttacctaaaa ggaagacgtt atggtgaaga agacaattgc agcgatcttt
     1981 tctgttctgg tgctttcaac agtattaact gcctgcaaca ccacgcgtgg cgttggtgaa
     2041 gacatttctg atggcggtaa cgcgatttct ggcgcagcaa cgaaagcgca gcaatagcaa
     2101 taagcaataa cggtacgaca ggtatgtcgt gccgtttgtt ttttctgcga tagtcactaa
     2161 ggtaatagtt gaaattcccc tgccacctgg caaaatatcc gttcaaccat aagctttgca
     2221 ggacgacctg caaacgcctc ttttcaccgg ggacggcccc aattctccgg agcctgatat
     2281 gtcctggatt atcttagtta ttgctggtct gctggaagtg gtatgggccg ttggcctgaa
     2341 atatacccac ggctttagtc gtttgacgcc gagtgttatt accgtgacgg cgatgattgt
     2401 cagtatggcg ctacttgcct gggcgatgaa atcgttacca gtagggacgg cttatgccgt
     2461 gtggacgggt attggcgcag tcggcgcggc tatcaccggc attgtgctgc tcggtgagtc
     2521 cgctaacccg atgcgcctgg cgagtctggc gttaatcgta ttggggatta ttggtctgaa
     2581 actcagcact cactaactac cgggctgctg tacccaaata aacttactga catcaaaccc
     2641 ttcccgggtc gcgactgcca gcatctcctg tttcacttcg tcagaaatgg ttggcgtgcg
     2701 ggagagtatc cacaggtagt cgcggtccgg gccgcaaacc agcgcatggc ggtattcccg
     2761 atcgagtgca ataacgttat aaccgccata gaaaggacca aagaatgaca ctttcagcgc
     2821 agcgcgagtt ggtgcgccgg taaagtacgc tttcccttca ctctgctgcc acattcctct
     2881 gtcagggtta tagcctttat taatgacatt caggccgccg tcatcacgca ggctgtatgt
     2941 tgcggtgact ttttccagtc cacgttcaaa acggtgatca aaacgggcaa tctcatacca
     3001 ggtaccgaga taacgtttgg cgtcgaaatt attcactact gttacgccac gcggcggcgt
     3061 aggagaactg caggcaacga ccagaaatgc agctgtcgct gcggcaacga gagggagcag
     3121 gcgcataaat gtttccttac tggttttttt ctaagtgtag atgacagcaa ggaaaagcgc
     3181 agaaaaggtc cgaaaattcg gacccgatga aattttactg tagagcgttg agaatctgcc
     3241 aggcggcggc gactctcgct ggattgggat agtttttgtt agccagcatc actataccca
     3301 gctctttttc tggaataaac gcgacatagc taccaaatcc gccggttgcc cctgttttat
     3361 gtacccatga tgcgcgtact gcaggagttg ggggcgtaat cgcttttacg gggtgtgctg
     3421 ccagtgcaat tttattgcca ctgccgttaa tgatgctgtc aggatttacc ggccagtcca
     3481 gcatttccca gcccaggccc tgatacatat cgccggtttg ccagtagcga gattgtgcca
     3541 gttgtatccc ttgctgaagt gttttgtcgt tgatatcacg gggattcata ttgcttcgta
     3601 cccagcaggc catatcttca atggtcgact tcacaccata agtttcagca tctaacgccc
     3661 ctggcgaaac atgaactgcc ttgccttcgc gatatcccca ggcgtaattc ttttcttctg
     3721 cgggcggtac attaatccac gtatggttga gtttgagtgg ctggaagaca cgtgtttgca
     3781 tcgcctgctc aaaactcaaa ccagacggct tcacagccag tgcgccgaac aaaccgatac
     3841 tggagttggc atacagacgt tgtgttcctg gcgcccatgc aggctgccag ttttgataga
     3901 agcgcagcaa gtcgcttgag gatttcacct catccggcac ctgcaatggc aggccgccag
     3961 cagtgtaggt tgcgagatgt aatagtgtga tcccattcca ctgtttagcg gtaagttcag
     4021 gccagtattt tgttgtggga tcgcttaact tgatttcccc tcgagcaata gcctcgccac
     4081 caagcacgcc cgtaaatgtt ttgctgaccg aacctaactc aaacaacgtt tgctgtgtga
     4141 cgggctgctt tttggcgatg tccgcatagc cccaggtaaa gtaataaggt ttaccctgat
     4201 aaattaccgc caccgccata ccggggatct tttgttgctc tataagcggg gtaattgtgc
     4261 gatgcacaat atcgttgatt tgttgagggg cagcaaatgt ggagcaagag gcggtaatta
     4321 ataaggcgca gagcgtcgtt ttgaacataa ggtctggttt ccatacaaaa cggcccgcca
     4381 taggcgggcc ggatttacat tggcgatacg ttagattgta acgataccaa tcagcgtgac
     4441 aactgtcagg atagcagcca gaccgtagaa aacccatttg cccgcaggca cgtgaatttt
     4501 cagatcgtgc atcgcgtggt gcatacggtg taaaccacac cacagcggca gaacgatcat
     4561 caggaacagg aatacgcgac caatgaagct ctgcgcgaac gccagaacgc gctcgtagct
     4621 cagcgcatca cccggaaaca gccccagtgg cagcagaata cccaccagca ggatcatcac
     4681 cggcgcaata attgcgctcc acataccacc ggccccgaag aggccccaga ataccggttc
     4741 gtcagaacgc tttggatttg gattaatcat ctcaggctcc ttaccagtac agggcaacaa
     4801 acaggattac gatggtggca accacagtta ccgcccagag acttttgata attggctctg
     4861 gtcccatttt ttcgtctttt acaatgatat tggccgcttt cggtgccagt tcaaaccagg
     4921 ttttggtgtg cagcagagct gccgccagag tgatcaggtt aatgatcacg ataaccgggt
     4981 tttgtaaaaa gtcgacgaat cccgcccagg cttccgggcc atttttcagg gcaaacagcc
     5041 cgaaaatcag ttcaatgctg aaccacacag ccggaaccgc cgtgccttcg cgcagcatgt
     5101 aaaagcgata aaacggcaat tttttccacc aggtggacgt cattggccgt acatacggtt
     5161 tacgtttagt cgtcatgttg cactccttag cgtggtttca gggtcgcgat aagaaagtct
     5221 ttcgaacttt ctactttgcc ctgctgaatg gccgcagccg gatcgacgtg tttcgggcag
     5281 acttcggagc aatagcccac gaaagtacag ctccatacgc cgttctggct gttcaactgc
     5341 gccatacgct ccttcttacc gtggtcgcgg ctatcttcgt tataacgatg cgccagcgta
     5401 atggcagccg gaccgatgaa ctctgggttc aggccaaact gcgggcacgc ggcgtagcac
     5461 agaccacagt tgatgcaacc ggagaactgg tgatactttg ccatctgcgc cggggtctgg
     5521 atgttagtac cctgatccgc ggtgcgggag ttgccgatga tgtacggttt gatcgcttcc
     5581 agactttcga tgaagtgggt catatcgacc accagatcgc gttcaatcgg gaagttagct
     5641 aacgcttcaa ccttcatacc gtcggtgtaa tcacgcagga aggttttaca tgccagtttt
     5701 ggcacgttgt taaccatcat gccgcaggaa ccacaaatcg ccatacggca ggaccagcgg
     5761 taactcaggt ccggtgccag gttgtctttg atgtagccca gcgcatccag taatgaggta
     5821 gttgcgtcat aaggcacttc atagaatgcg ctatgcggtg cggtatcgac ttccgggtta
     5881 tagcgcacca cctcaatttt caggtttttc atctcagcca ttcgccttct ccttcttatt
     5941 ggctgcttcc gccttatcgg ctgcatccgc ttcgccaccg taaacgcgtt tagctggcgg
     6001 cagcgtagta atcttcacgt cgctgtactc caggcgagtc gtgccatcag catcgcggaa
     6061 ggcgagggtg tgtttgagga agttgacgtc gtcacgctcg gtgcaacctt cgtccagacg
     6121 ctggtgtgcg ccgcgggact ctttacgtgc cattgcggag tgcgccatac attcagcaac
     6181 gttcagaccg tggcccagtt caatggtgta gagcaggtcg gtgttgaaca cgctggaggt
     6241 gtcggtgatg cgcacgcgct tgaagcgttc ctgcagctct gccagcttgt cgatggtttt
     6301 ctgcatcagt tccggcgtac ggtagatacc gcaaccttct tccattgcca ggcccatttc
     6361 gtcgcggatc ttcgcccagt tttcgccgcc atcctggtta accagatctt tcagacgttg
     6421 ttcaacgcca gctgcctgcg cttcaattgc cgcttcgttg ccattaccgg cagttgctgc
     6481 acgctctgtc gcttgttcac cggccagacg gccgaagacc accagttccg ccagggagtt
     6541 ggagcccaga cggtttgcac cgtgcagacc aacagaggaa cattcaccca cggcgaacag
     6601 acctttaatg cgggtttcac agttctgatc ggtttcgata ccgcccatgg tgtagtgtgc
     6661 ggtcggacgt accggaatcg gttctttaac cggatcgacg ccaacgtacg ctttcgccag
     6721 ttcgcagatg aacggcagac gttcatgcag ttttttctcg ccgaggtgac gcaagtcgag
     6781 ataaaccaca tcgccacgcg gcgtggagat ggtgttgcct ttacgccatt cgtgccagaa
     6841 ggcctgagaa actttgtcgc gtggacccag ttccatatat ttgtttttcg gctcgcccag
     6901 cggagtttcc gggcccatgc cgtaatcttg cagataacgg tagccatttt tgttgaccag
     6961 aataccgcct tcaccgcggc agccttcggt catcaggata ccggaacctg gcagaccggt
     7021 tgggtgatac tgaacgaatt ccatgtcacg cagcggaacg ccgtggctta gcgccatacc
     7081 cataccgtca ccggtaacga tgccgccgtt ggtgttgtaa cgataaacgc gacccgcacc
     7141 gccagtagcc ataacgaccg cgttagcacg gatctgcacc agcgtgcctt ccatcatgtt
     7201 cattgctacc aggccgcgaa catgaccatc atcaaccaga atatccagca cgaaatgttc
     7261 gtcaaaacgc tggatctgcg ggaattgcag agaggtctgg aacagcgtgt gcagcatatg
     7321 gaagccggtc ttatcggcgg cgaaccaggt gcgctcgatt ttcatgccgc cgaagcgacg
     7381 tacgttgacg ctaccatccg ggcgacggct ccatgggcac ccccacagtt ccagttgggt
     7441 catttcggtt gggcagtggt ggacgaaata atccacgaca tcctgctcac acaaccagtc
     7501 gccacccgct actgtatcgt gaaagtgata ttcgaagctg tcatgatcct gcgcgacagc
     7561 ggcggagccc ccttctgcag caacggtatg gctacgcatc gggtatactt ttgagattag
     7621 tgcgattttt gcatttggat ttgcctgcgc ggcagcaatt gcagcacgta atcccgcgcc
     7681 accggcgcct acaatggcaa gatcggcttg aaaggtttgc acgacattcc tccagattgt
     7741 ttttatccca cagccccgta cttcagggta agtgcctgaa agttacggtc tgcgaacgct
     7801 attccactgc tcctttatag gtacaacagt atagtctgat ggataagtct gaaatttgac
     7861 gagatcgatt tttttagtgc gcaagagggc taaattatca ctgaagatga ttaatttaat
     7921 tactaaacca tcagatcgtg ctttttttag tcactgccca ccgctgtttg attcctgcgt
     7981 aaaatttgtc tcgccgtcgc gttgcgagta gacttcgtgc ccttgtcaaa aactggagat
     8041 ttaactatga gcgaaacggc atcctggcag ccgagcgcat ccattcctaa cttattaaaa
     8101 cgcgcggcga ttatggcgga gatccgtcgt ttctttgccg atcgtggagt gctggaggtg
     8161 gagacgcctt gtatgagcca ggcgacggta accgatattc atttggtccc gtttgagaca
     8221 cgtttcgttg gccccgggca ttcgcagggg atgaatctct ggttaatgac cagcccggaa
     8281 taccatatga aacgcctgct ggttgccggt tgtgggccgg tattccagct gtgccgcagc
     8341 ttccgtaatg aagagatggg gcgttatcac aaccctgagt tcactatgct ggagtggtat
     8401 cgaccgcact atgatatgta ccggttgatg aacgaggtgg acgatctctt acaacaggtg
     8461 ctggactgcc cggcagcaga aagcctttct tatcaacaag ctttcttgcg ttatctggaa
     8521 attgacccgc tctctgccga caaaacgcaa ctgcgggaag tcgcagcgaa actggatttg
     8581 agcaatgttg ctgataccga agaagaccgc gacacgctgc tacaattgct gtttaccttt
     8641 ggcgtagagc caaatattgg caaagaaaaa ccgacctttg tgtaccactt tccagccagc
     8701 caggcatcac tggcgcaaat cagtaccgaa gatcatcggg tcgctgaacg ctttgaggtt
     8761 tattataaag gtattgagct ggcgaatggt ttccatgaat tgacggatgc ccgtgagcag
     8821 caacaacgct ttgaacaaga taaccgtaag cgcgcggcgc gcggtttgcc gcagcacccc
     8881 attgaccaga atctgattga agccttgaaa gtcggtatgc ctgactgttc cggcgtggca
     8941 ttaggtgttg atcgtctggt gatgttggcg ctgggcgcgg agacactggc tgaagtcatc
     9001 gcctttagcg ttgaccgggc ataattctga aaaattactg aattaacagc gaagaatggc
     9061 gtgataagcg ccattctgta gcatatgttt tcatttttcc gttaaaggcc acaatttaag
     9121 actggatatt tgatatcatc caggtatcaa ttctgtattg gtttttacgc tgcctgctca
     9181 atgttgtgca gtgcagttca gtgggcacgt gttatacacg cgctgaaatg aaggatggtt
     9241 tcatgcctca cacgataaaa aagatgagtc tgataggact catattgatg atctttactt
     9301 ccgtatttgg atttgccaat agcccatcgg cttattactt aatgggttat agtgcgattc
     9361 ccttttatat attttctgca ttgttattct ttattccatt cgccttaatg atggctgaaa
     9421 tgggagctgc ttatcgcaaa gaagaaggcg gtatctattc ctggatgaat aatagtgtcg
     9481 gaccacgttt tgccttcatt ggtacgttta tgtggttttc ctcttatatc atctggatgg
     9541 tgagtacctc cgcgaaagtt tgggtaccgt tctcaacatt cctctatggt agcgacatga
     9601 cccagcactg gcgtattgct ggactggagc ctacgcaggt ggttggtctg ctggcagtgg
     9661 catggatgat tctggtcacc gtcgttgctt caaaggggat taataaaatt gcccgcatta
     9721 ctgcggtggg cggtattgca gtaatgtgtc tgaatttagt attgctgtta gtaagcatta
     9781 ctattttgtt attaaatggt gggcatttcg cgcaggatat taatttcctt gcatcaccga
     9841 acccaggtta tcagtccggt ctggcaatgc tatcgtttgt ggtatttgct atttttgcct
     9901 atggcggaat tgaagcggtt ggtggtctgg tcgataaaac ggaaaatcca gaaaagaact
     9961 ttgccaaagg tattgttttt gccgctattg ttatttcaat cggttattcg ctggcaatat
    10021 ttttatgggg tgtcagcaca aactggcagc aggtattaag taatggttcc gttaaccttg
    10081 gcaatattac ctatgtgctg atgaagagcc tcggggtgac gctgggtaac gcactgcatt
    10141 tgtcacctga agcgtcattg tcgctgggcg tatggtttgc gcgtattacc ggactttcga
    10201 tgttcctcgc ttataccggt gcgttcttta cgctttgcta ttcaccgctg aaagccatca
    10261 tccaggggac gccgaaagcg ttgtggccgg aaccgatgac gcgcctgaat gcgatgggga
    10321 tgccgtctat cgccatgtgg atgcagtgcg ggttggttac tgtcttcatc ctgctggttt
    10381 cgtttggtgg cggtaccgca tccgcgttct ttaacaagct gacgctgatg gcgaacgtgt
    10441 ctatgacgct tccttacctg ttcctcgcgc tggctttccc attctttaaa gcacgtcagg
    10501 atctcgacag accatttgtg attttcaaaa cgcgtatgtc ggcaatgatt gcgacggtgg
    10561 ttgtcgtact ggtggtgaca tttgcgaacg tcttcaccat tattcagcct gtggttgaag
    10621 ccggagactg ggacagcaca ttgtggatga ttggcggccc tgtcttcttc tcgctgttag
    10681 cgatggcgat ttaccagaac tattgcagca gagtggcaaa aaacccgcag tgggcggtgg
    10741 aataacccga tgccctatcg tctgcctgac gatagggcgt aaagacagga aatgttatgg
    10801 atgacaccag ccgggacccg gcaataaccg aagatgaaat cagggcgctg caattttctg
    10861 ccggggatgt agcggaaata gagcagacga ttctctcttt tgtcgatgcc tgccacaccc
    10921 gtaaagtggc gatggtggtg ggtagcacca ttaacaccct caaagatcgc gatggtaaac
    10981 ggtggggaaa cctgccggat atttattgcg cttatcttat ccgatgtctg gtttttcgcg
    11041 gggaattagt gggatacggc gatttgtttc gtatgcgcta tagcgaaatt aagcgaccgg
    11101 ttaccttatg agtgcgcgca tgtttgtctt gtgctgcatc tggtttattg tggcgttcct
    11161 ctggataacc atcacttccg cgctggacaa agagtggatg attgatggac gcgggattaa
    11221 caacgtctgc gatgtgttga tgtatctcga agaagacgat acccgtgatg ttggtgtgat
    11281 aatgacgtta ccgctctttt tcccttttct ctggttcgct ttgtggcgaa aaaaacgcgg
    11341 ctggtttatg tacgccaccg cgctggctat tttcggctac tggctgtggc agttttttct
    11401 gcgctatcag ttttgtttgt gagccggatt ggttcatccg gcacacaaac ttacaaactt
    11461 cccgcctgac gacctttgcc cgcagacgtc agcgttctcc ccgtttgttt accgttgagg
    11521 ctttccagac gcatctggaa gggcgggaat ggcatatcga taccgtgggc gtggaagcct
    11581 gccagaatca gctggtggat ctcatggcgt agcggcatac ggtgacccat ctcagcggcg
    11641 taaatacgca gctcgaaaat ctgaatcccc tgttgcagat ccaccaggaa gacttccggt
    11701 gccgggttgt cgatcaccag cgagcagcga cgcgctgcgg tgagcaggat ttccgtcact
    11761 tcttcgctat tggcatcggc aggggccggt atcgtcaaca ccacgcgcgt gaccgagtca
    11821 gagagcgacc agttgataaa ctgctcggta ataaacgcct tgttcggcac gattatctct
    11881 ttacggtccc agtcgctgat ggtggtggcg cgggtgttaa ttttcgtcac gctaccggtg
    11941 agatcgcgaa ttgtcaccgt atcgccaatg cgaatcggtt tttcgaacag gataatcaga
    12001 ccagagataa agttggcgaa aatttcctgc aaaccaaaac cgagaccaac accgagcgcg
    12061 gcaaccagcc actgcaattt cgaccactca ataccaatca ttgagaagcc gaccagcccg
    12121 ccaatcagca tcagcagata tttggtgatg gtggtgatgg cgtaacccgt acccggcgtt
    12181 aaatccaggt gctgcaaaat cgccagttcc agcagcgctg gcaggttgcg caccagctgc
    12241 gtggtgatga taaataccag aatggcaatt agcactgcac cgagggtaat cggctccagg
    12301 ctttctacgc cctgtacagt ggaagtgaca tcccacagag aaatattttc gaggaagccg
    12361 aaagcggaat ggatttctga ccacagcaca atgaccgaaa gcagggcgat caacatcaaa
    12421 attgagcgca ccagccgcaa ggattgcgca ctgatggcat cgagatcgac ttcgctttca
    12481 tcgacttcaa ttgccccttc cgggctactg tgatgatgcg cttcctcttc gccacgcgcg
    12541 cgttgcgcta acatctctgc ccggcgatgc ttcgcccggt caaacgccag cctgcgacgc
    12601 tggatcagca tccagcggcg gataacgtga taaaccacca gtagcaggaa ccagatggca
    12661 accgaggttt caagcctcgc taacagcgcc tgtgccgttg ccagataacc cacagccgac
    12721 gccagaatgg caaccaacgg cgcgccaatc atcatgttcc acagcatatg gttggtaatg
    12781 ttgtcgccgc tgccctcttt gttgagatac agtgggatcc cggccttttt caggctgagg
    12841 gtgaccaccg ccagcgcacc gcaaatgagg ataaagcaaa gccgtcccag cgaaccggag
    12901 aattcacggt cgtcgaggtt atcgaacatc atcagcgcca taatcagcgg cacaataagc
    12961 ccgatgctca tcaggtagta gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa
    13021 tgagcgataa acaagccgtt cgggcgggca aaggtggcgc aaatcatcac tacccacagc
    13081 agcggcacgg tggccgttac gccatcgcca atcgcgaccg ccagcggata aggccacgcc
    13141 tcgcgcaagc cgtaacccag cgtcatccac agcaccggca gcggtgacgc gacgagaatc
    13201 gaccagaaaa gagtgcgcaa cgtcagccag aagtgatcct gagtcacttt gccgactttc
    13261 gccgccgaac gttcaagaaa acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc
    13321 agaatcaacg cgccaaacag cggcaaaatc gtctctttgc tggtcagcat catcacactg
    13381 gctttgccca actgactgaa ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt
    13441 tccagtggcc aggcgatggt catcgggcgc acgtcagagg tccagaacag atagcgatgc
    13501 gtcgcttcat tcacctcttt cagcgcatcc tcaagttgcc cgttggagac tttcagcttg
    13561 gtcagttcca gcagtagcgt gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga
    13621 gtgcgtagct gtgcttccag aatacggttt tgctcggcgg tcagcggctg accgtcggcc
    13681 tgatgaattt gccgtagcag cggctgttta ttaagcagat cctcataacg taaccgttgc
    13741 acacgcaact gcgccatttc ggtatcaagc tgttgtggtt tcggcatttc cggcagccgt
    13801 gccacctgcg cccgcaacgc ttcgccgagc aggttggacg atcccagcca ttgcgactgt
    13861 tcacgcagcg tattcagcgc ctgccgaacc tgtaacgtct ggctggtggc ctggcgctgt
    13921 tgcgaggcaa cgagatccat ccgctgcgcc tgttgattca gtgccgccga tagttcgcgg
    13981 ttaattttga attgcgcgac gatgtctttc ggcaaatcgg cgctgttttc tgccagcaat
    14041 tcggtacttt ccagcgcccg ctccgcctca agctgacgtt ggctgttcaa ctgattacgt
    14101 aaggcctgca aatacgcatc cagttgctgg ctctcttttt ccgccagctc tgagcgtaag
    14161 cgcgctaatt cctgacggtt attggcagac agctgcgcca gttccagttc atcaacgagc
    14221 gctttcaggc gtgcggagtc agactgcaac gcgaaatttt gtgcctgatt gagcggggta
    14281 ttgccagtaa gcgttcccag gcggcgctcg atctcattta actgacggcg ggcgtcggtt
    14341 tgctgttgcg gcagttgatt cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc
    14401 tgggcctgac ggcttttatc cagcaactgg ctgctgacct ggagaatttc ctgattcagc
    14461 gcgtcggtag acattcccgg cgacacgctg cgcggctcgt cacgcatgtt gtttaattgt
    14521 gcgcgcagag tagcggagag tttcggataa ttatcgataa cttgctgata ttgtttgatg
    14581 cgctcaaggg aaccttttcg ttcctcaagc gcatttaagg cagactggag cgcctctacg
    14641 acttccggct gtgcgggttt cgccgctttt gcctgctcca gttcctgagt gatttgtttg
    14701 ctatcggggg ccgtcgcggc gtacgccccc caactgaggc accaggccat cagaaaagtg
    14761 ataatcaggc gcacgtcagc gtttcctttg atggattaga cctggtcttt tttatcgtca
    14821 accaatgggc tggcgtcgtg ttctgcttcg atctcttcag caggaagtgg ggcgggttca
    14881 gcgtctggcg taacaaaggt ttcggtagat actgccagcg gctggccaat tttcgtgaca
    14941 gacaggcttt ccagttgctc aaccagattc actttacccg gtgcaaacag gttgataacg
    15001 gtggaaccga gtttaaagcg acccatttcc tggcctttca gcagtgccac agaaccgtcg
    15061 ttttccccgg caggccaggt ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg
    15121 cccgcccaga ccgtctcaat gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt
    15181 gggccaaatt cggtatcgaa aaggcaaatc acgcgttcgt tacgggcaaa cagattcggc
    15241 acgttctgag ccgtgagatg gttaacggag aagagatcgc ccggtacgta gatcatctca
    15301 cgcagaatac cgttgcacgg catgtgtacg cgatggtagt cacgcgggga gaggtaagtg
    15361 gtcacaaacg taccgttgcg gaacaggtcc gccatcagat agttgcctgc cagcagggct
    15421 tcgaggctgt agttgtggcc tttggcttgc aggattttat cttcttcgat tttacccaac
    15481 tggctgataa cgccatcggc aggcatgacc agtacattcg gatcggtatc gattgggcgt
    15541 acttcgtcac gcagcggacg gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc
    15601 ggcttttgcg cctctttcat gtcgaccttg tagtatttaa cgaacagatc gataaccagt
    15661 tttgtcagcc atcctgcccg cttgcttgcg ccccaacccg ccaggcgagt aagccatagt
    15721 ttcggcagaa tgtactgtag cgaaagttta aatgaattta acaaggtagc ctccaggcca
    15781 ttgttttgtc gttcctgatc cggccaacat gccggatcct gaaaaaaagg ggacgattct
    15841 aacgacggtt agcttaattg tcagtcatcc gtatcagaaa agtttttacg cgtttttacc
    15901 tgcgccatgc tttccagaat acggtgatag ttttcgaaac gggtttccgc gattttccct
    15961 tcctcaaccg cttcccggat agcgcagccc ggatcggtat cgtgtttgca atcgcgatat
    16021 ttacacagac ctaaatagtc atggaattcg acaaagccct gagtgatttg ttccggctcc
    16081 aggtgccaga ggccgaactc acgcacacca ggggaatcaa tcacatcacc gccgtgcggg
    16141 aagtgataca gccgagcggc ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca
    16201 tcgttggtca ggatctcttt ttgcagcccc agcaacgcat tcagcaggct ggatttgccg
    16261 acgccagact gcccggcaaa aatgctgatg cgcccggtca acgcctcttc cagcggtttt
    16321 agcccatcct gagtatggct ggaaaccatc aatacgcgat aaccgatatt gcggtagata
    16381 tccatctgct cgttgacgaa cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc
    16441 acaataatcg gctcaatctg caaggtttcg caggccacca ggtaacggtc gataatattg
    16501 agcgacagct ccggcaaaat ggcggagaca atgacaatct ggtcgatgtt ggcggcaata
    16561 ggttttacgc cgtcgtagaa atccggacgc gtcaacaccg aggtacgctc atgcaccgct
    16621 tccacgatcc ctttgacatt tacgccttcc gccgccggtt tacccggacg ccagactacg
    16681 cggtcgccgg ttaccagcga acggatggta cggcgaatat tgcagcggtg aacgtcgcca
    16741 tcggcggatt ccacatcagc gtgcatacca aagcggctga tgacgatacc ttcatcaggc
    16801 tcgccaaaca gattgtcgtc gtagtcgggc ttctccttag acgttttaag acgacgctgg
    16861 tgattggcgt tcacgcggcg ctgctggcct ttggagagtt tatttttact caatcgtaca
    16921 gactcctggt cgcccctgat gggcaaaaca tctatgatac acgcaattgt ggatcaatat
    16981 agtcactgtg aatgggtgga aaatatcatg agtgccaatg aaaacaacct gatttggatc
    17041 gatcttgaga tgaccggtct ggatcccgag cgcgatcgca ttattgagat tgccacgctg
    17101 gtgaccgatg ccaacctgaa tattctggca gaagggccga ccattgcagt acaccagtct
    17161 gatgaacagc tggcgctgat ggatgactgg aacgtgcgca cccataccgc cagcgggctg
    17221 gtagagcgcg tgaaagcgag cacgatgggc gatcgggaag ctgaactggc aacgctcgaa
    17281 tttttaaaac agtgggtgcc tgcgggaaaa tcgccgattt gcggtaacag catcggtcag
    17341 gaccgtcgtt tcctgtttaa atacatgccg gagctggaag cctacttcca ctaccgttat
    17401 ctcgatgtca gcaccctgaa agagctggcg cgccgctgga agccggaaat tctggatggt
    17461 tttaccaagc aggggacgca tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg
    17521 gcttactacc gcgagcattt tatcaagctg taaaattttt cctggtcacg taagcgccgg
    17581 aatccacttg cggcggcgtg attttgacgc taaattgaac gtcttgctga taatttcagc
    17641 acttgagata aaaacgcaaa aaaaactttt ttggggggtt gcagagggaa agatttctcg
    17701 tataatgcgc ctcccgtaac gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag
    17761 tacgcgggaa tagctcagtt ggtagagcac gaccttgcca aggtcggggt cgcgagttcg
    17821 agtctcgttt cccgctccaa aatttgaaaa gtgctgcaaa gcacagacca cccaagcggg
    17881 aatagctcag ttggtagagc acgaccttgc caa
//
LOCUS       DAZKIV010000095        17848 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZKIV010000095 DAZKIV010000000
VERSION     DAZKIV010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.095"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(582..1583)
                     /locus_tag="ACHOT6_003092"
     CDS             complement(582..1583)
                     /locus_tag="ACHOT6_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFP9948267.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1690..1986
                     /locus_tag="ACHOT6_003093"
     CDS             1690..1986
                     /locus_tag="ACHOT6_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9948268.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(2120..2545)
                     /gene="arsC"
                     /locus_tag="ACHOT6_003094"
     CDS             complement(2120..2545)
                     /gene="arsC"
                     /locus_tag="ACHOT6_003094"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFP9948269.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2558..3847)
                     /gene="arsB"
                     /locus_tag="ACHOT6_003095"
     CDS             complement(2558..3847)
                     /gene="arsB"
                     /locus_tag="ACHOT6_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFP9948270.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3901..4254)
                     /gene="arsR"
                     /locus_tag="ACHOT6_003096"
     CDS             complement(3901..4254)
                     /gene="arsR"
                     /locus_tag="ACHOT6_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFP9948271.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4994..5077
                     /gene="dinQ"
                     /locus_tag="ACHOT6_003097"
     CDS             4994..5077
                     /gene="dinQ"
                     /locus_tag="ACHOT6_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFP9948272.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(5131..6483)
                     /gene="gorA"
                     /locus_tag="ACHOT6_003098"
     CDS             complement(5131..6483)
                     /gene="gorA"
                     /locus_tag="ACHOT6_003098"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFP9948273.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6555..7397)
                     /gene="rlmJ"
                     /locus_tag="ACHOT6_003099"
     CDS             complement(6555..7397)
                     /gene="rlmJ"
                     /locus_tag="ACHOT6_003099"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFP9948274.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7600..9642
                     /gene="prlC"
                     /locus_tag="ACHOT6_003100"
     CDS             7600..9642
                     /gene="prlC"
                     /locus_tag="ACHOT6_003100"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFP9948275.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9650..10402
                     /gene="rsmJ"
                     /locus_tag="ACHOT6_003101"
     CDS             9650..10402
                     /gene="rsmJ"
                     /locus_tag="ACHOT6_003101"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFP9948276.1"
                     /translation="MKICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10451..11920)
                     /gene="dtpB"
                     /locus_tag="ACHOT6_003102"
     CDS             complement(10451..11920)
                     /gene="dtpB"
                     /locus_tag="ACHOT6_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFP9948277.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            12086..12139
                     /locus_tag="ACHOT6_003103"
     CDS             12086..12139
                     /locus_tag="ACHOT6_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948278.1"
                     /translation="MVLNVVLAGGFDCVRRS"
     gene            complement(12238..12672)
                     /gene="uspA"
                     /locus_tag="ACHOT6_003104"
     CDS             complement(12238..12672)
                     /gene="uspA"
                     /locus_tag="ACHOT6_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFP9948279.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            13063..13398
                     /gene="uspB"
                     /locus_tag="ACHOT6_003105"
     CDS             13063..13398
                     /gene="uspB"
                     /locus_tag="ACHOT6_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFP9948280.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13469..14968)
                     /gene="pitA"
                     /locus_tag="ACHOT6_003106"
     CDS             complement(13469..14968)
                     /gene="pitA"
                     /locus_tag="ACHOT6_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFP9948281.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            15200..16402
                     /gene="yhiN"
                     /locus_tag="ACHOT6_003107"
     CDS             15200..16402
                     /gene="yhiN"
                     /locus_tag="ACHOT6_003107"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFP9948282.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16718..17770)
                     /gene="yhiM"
                     /locus_tag="ACHOT6_003108"
     CDS             complement(16718..17770)
                     /gene="yhiM"
                     /locus_tag="ACHOT6_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFP9948283.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4664 a         4466 c         4554 g         4164 t
ORIGIN      
        1 ataaaattcc ttattcatca tttgttttat gctaagatgt ttaaattgga taattagaac
       61 aggaatggat tttcttctac acacaataaa tgtaatgcgt tatatcttcc tgatataatt
      121 acctttaaat tccctttcat ttccacccgg taatatttgt gtataaaaaa aggcagtgat
      181 agtatcgtaa aggaaaacgt taacaactat caaaaagcgc tcattttaaa aagttattat
      241 gttctccgat agtgtgtgaa gtaatatttt tgtgatgttt tagatgtgta ataatgaacc
      301 aggttaaagt gtatttaaaa atgtgttgta ttagcgactg aaatctgcct tttaaaaagg
      361 aggagggtac tgagacaaag agtcattgct tctccgataa aatgtcagag aagcatgatt
      421 acgtggttat tgtctgttga tgacttgttg accgagccgt catcattctc aatgcattag
      481 ttaaatcatt tgtgattttt aaggattatc ctgtctatga ttttctactt agaaggatat
      541 ggcactaact atgcatctta tagtttactg ctggcatcta tttaaaatag caattgagtc
      601 aaatagccca tcaagatagc catacctaat ataaccgtca ggaatgcagc tatcatcggc
      661 attctgaaca ttgatttaag taatatcact tccgtcaggc ttgcgccggc actgccgatg
      721 attaatgcca ttaatgctcc cagacccatc ccttttgtca tcagaacaga tgccagaggg
      781 ataactgcct cagcccggat atacagcgga ataccgacaa cggcgcttaa tgggatggcg
      841 aagggattat ctgctcctgc atgagcggca atccacgctg agggaataaa gccataaata
      901 aaagatccta ttaatactcc taacaaaaga tatggcagta catctttaaa ctgttgcaac
      961 gcatctttgg tggctagttt tatgggatta atacttacta cagctttggt actgcaacag
     1021 gtaactacgg ggttctcaat ggctttagcc gctggactac aacaactcac ttttatgggc
     1081 gtatatgtcg ttcccggcga agtttttgct ggagcacaac aatttgctga tgagctttta
     1141 ctggcaatga tatgacgttc aaaccccaga gaatccagga taatactggc aagaacggag
     1201 acgccggctg cgataatcgc gtacaacaag gtgactttcc aaccaaaggt catccacatt
     1261 agtccgacga taatgggatt aagtaatggt gaaacaaata aaaaagtgag agtcggacca
     1321 aacccggctt tcgctgacaa caatccacgt aacatgggta ttgtcgagca actacagaac
     1381 ggggtaacgg ctcccaacag agcagccagg agatagcctc tcccttttct ggcccccatc
     1441 atctgctgga ttttatggtc tggcaccttt tgtcttatca ggctgacacc ggcactaatc
     1501 actataaaca gcaacgaaag ctcgacggcg agaaacacaa acatctctgc ggcatcttgc
     1561 agcatcgcaa gccaactact catacgtaac ctcattggat aaatctatat ttccagaata
     1621 atcgaaatga aattcagatc aagatatttc tggtattatc gaaatatcaa ccagacagag
     1681 gggtgtcata tgcagttgga agaagtcgct aaagcgctga aagagctagg tcacccgacc
     1741 cgtttgttca tatttaaaca tcttgtcaaa gccggggaac aagggttgcc ggtcggtgag
     1801 ttgcaaaagc agcttgggat acctggctcc acactcagcc accatatatc agcactggta
     1861 tcggtaggac tggtcaaaca aaaccgtgag agtcggacgc tgatgtgtgt ttcgcaatac
     1921 gcaatgctgg aagcgataat tgaattctta cgcgaggagt gctgcgtaaa tagcaaaatt
     1981 gcctgatgcg ctacgctcta catatttcat gccatatatt gaatttgcac ggttttgtag
     2041 gtcggataag acgcgttagc gtcgcatccg acataaacaa agcgcacttt accaactatc
     2101 ggcagtggat atcgccgctt tatttcaggc gcttacccgc ttcatcaacc actttctcgc
     2161 catcttcctt ggagaatgcg cctttttgcg catctggcag aatttccagc accacttctg
     2221 aagggcggca caggcgagtt cccagcggcg tcaccacaat cgggcgatta atcagaatcg
     2281 ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa tttatcttcc gcaaggccca
     2341 gctcctcata cggttcgacg tttttacgca gcagcgcgcg tacggaaatc cccatatcgg
     2401 caatgagttt gaccagttca tcgcgcgttg gcggagtttc cagataatgg ataatggtcg
     2461 gttctgtgcc gctgttgcgg atcatctcca gcgtattacg cgacgtgccg caggccgggt
     2521 tgtgataaat ggtaatgttg ctcatatcag tatctcatta caaagtgaaa gagagacgta
     2581 gcgccagcgc agccagcgtc acaaacagca caggcagggt catgataatc cctgtacgga
     2641 agtaatatcc ccagctgata gtcatattct tctgcgaaag tacgtgcagc cagagtagcg
     2701 tagccaggct accaattggg gtaattttcg gtcccaaatc gcagccaatc acattggcat
     2761 aaaccatcgc ttctttgata acgccagatg ccgtgctgcc atcaatggac aacgcgccaa
     2821 ccagtaccgt cggcatattg ttcataatag aagagaggaa ggcggtgagg aatccggtgc
     2881 cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt gagtacgcca gaaagatatt
     2941 ccgttaatcc ggcattgcgc aggccataaa ccaccagata catgccgagc gagaagatga
     3001 caatctgcca gggggcaccg cgcaggactt tacccgtatt aatcgcatga ccgcgtttag
     3061 cgacgacaaa taatatcagc gcgcccacag ctgcaatggc gctcaccgga atgccgagcg
     3121 gttccaggac gaaaaatccc accagcagaa gcagtaaaac aacccagcca gctttgaacg
     3181 tagcaggatc tttgatcgct tctgcgggag atttcagcag cgccatatcg tagttctgcg
     3241 gaatatcttt gcgaaaatag agatgtaaca tcaccagcgt ggcaacaatc gcggcgatat
     3301 ccaccggcac catcaccgag gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa
     3361 cgatattcac caggttggag acaataagcg gcaggctggc ggtatcggca atgaatccgg
     3421 ccgccatcac gaacgccagc gtagtgcctt tactgaaccc taaagccagc agcatggcga
     3481 tgacaatcgg tgtcaaaata agcgccgcgc catcattggc aaacagggcg gcaacggcag
     3541 caccgagcag gacaatccag gtaaacagca agcgaccacg accattaccc cagcgtgaga
     3601 cgtgcagcgc cgcccattca aaaaagccgg actcatccag cagcaggctg atgataatga
     3661 cggcgataaa cgcagccgtc gcgttccaga cgatattcca caccaccgga atatcacccg
     3721 gatggaccac gcccgtaact aacgccagta ctgcgccgag cgttgcactc cagccgatgc
     3781 ctaaaccttt cggctgccag ataaccaata cgatggtcag gacaaagata gcgcctgcca
     3841 gtaacataat gcctcccgga taaaacacat ctgaaaattc atatgtgttt agctaaattt
     3901 ttaactgcaa atgttcttac tgtccccgga acagttttgt cgagctaggt tgcggacaat
     3961 cgcctgaacc ttttcctgtt cacatcgcca ggcctcatca ataattttcg ccgcccatgc
     4021 tggaatatgc ggtgataagc ggtaatgaac ccacttacct tgcttgcggt ccagcaatag
     4081 cccgctttca cgcagcaatg ccaggtggcg ggagatcttg ggctgcgact ggtcgagagc
     4141 agtgcagaga tcgcagacgc ataactctcc cagttcgctg agcagtaaaa cgatgcccag
     4201 acgggtttca tcagcaagaa ttttgaacaa ttggatgggt aacagaaatg acatattgcg
     4261 ctcctgattg ttgcaggtag tgtctctctt cgaagcggat aagtcaaaaa catatatgac
     4321 ttaacgaatg tgtaagtgca gaggaaggta ataggtgtga attttgagtt ggctattcat
     4381 ttgaaaggag gtctgaatca ggacgtaaaa aaaccgactt tgcgtcggtt ttttactttc
     4441 cagccctgag ttggtggctc tggctggagt gagagagttc ttatctaaca gctcaatact
     4501 attagccagt ggctaacatt gcaagaatta gcgatttctt cagctggcgg agggagcact
     4561 tcggtatata tcgcagtgac gatttatata ctttcactgg gtcatcgtca tattaagcct
     4621 tgtccgaatc cggatgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga
     4681 gttggtggct ctgactggag tgagatagcc atcatctaac gcatcaatgc taataccata
     4741 ctgaaactat tgcaaggacg tgctggtttt ataacctgca tgtactgtat gattatccag
     4801 ttagctctga ggcattttca ctctggcaat gcgcataaac gctttcaaag tcctggtcag
     4861 aagtacgggt ggtgccgtta actgatgctc tggccggagt gagagagttc ttatctaaca
     4921 atgagacatg cgccgtgaca ggcagtggat gagtaagcgg atgcattctc actccatcgc
     4981 atggagaaaa cgggtgattg ataaagcaat catcgttcta ggggcgttaa ttgcgctgct
     5041 ggaactgatc cgctttctgc ttcagcttct gaactgatag cggaaacgta attaagggct
     5101 aagagcacac tactcttagc cctttaacat ttaacgcatt gtcacgaact cttctgccgc
     5161 cgttgggtga atggcgacgg tattgtcgaa gtcttttttg gttgccccca tcttcagcgc
     5221 caccgcgaag ccctgcaaca tttcgtccat accaaagcca atgccgtgaa taccgacaat
     5281 cttctcttcc gatccaacgc acaccagctt catgcggcac ggctggcggt gagtggtgac
     5341 ggcggtatac atcgcggtga aagaggattt atacactttc acctgatcgt cgccatactg
     5401 ctcgcgcgcc tgcggttccg ttaaaccaac agtaccaatc ggcggatggc tgaagaccac
     5461 ggtcggaatg ttgctgtaat ccagatgctc atccggcttg ttattaaaca ggcgttcaga
     5521 gagacggcga cccgctgcaa ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc
     5581 caccgcgtaa ataccttcaa tattggtgtt ttgatattta tcgacgacga tatagccttt
     5641 ttcgttagtt ttaacgccag cggcttccag gttgatgttg tcattggcag gctcgcgacc
     5701 aatcgcccaa atcaggcaat ccaccgtttc actgcgacca tcttccagct ccagcgtcag
     5761 gctaccatcg gcatttttca ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc
     5821 ttcggcgttc atcacttcga ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg
     5881 cgcatgttta cgcacaaaca gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag
     5941 ctcaacggcg atgtaacccg cgccaacaac cgccacgcgc tctggcaaag caggaagggc
     6001 gaagaagcca tcagaatcaa taccgtattc cacgcccgga atatccgggt ggctcggacg
     6061 accgcctgtg gcgatcagaa tatgatcggc cgtgatggtt tcgccgttta cctccagcgt
     6121 tttggcatca acgaagcggg caaagccttt gattacatca acgttatttt taccgagcac
     6181 gttttcatag gaagtatgaa tacggtcgat ataggcggta cggctggcga tcaacgtttc
     6241 ccagttgaat ttattgatag tggtatcaaa accataatcc gggccgtaca tatggatcgc
     6301 ttcacggatt tgcgccgcgt gccacatcac ttttttcggc acacagccaa catttacgca
     6361 ggtgccgccc agctctttgg cttcaatcag cgcacatttc tggccgtaca tagccgcgcg
     6421 gttgatggag gcgataccgc cgctgccgcc gccgatggcg atgtaatcat agtgtttagt
     6481 catgacaaag tgtccttatc gttgattacc gcgattgtag cgcgagacgt aataggtgcc
     6541 agcaatggct gcaattactc cggcacgatc cagcttacgg tggcgtgccc ggtgcctgcc
     6601 ggaaccagtt tgctgtgcag ccacggcagc acgttattca tctgttgttc cagtttccac
     6661 ggcgggttaa tcacaatcat gccggaagcg gtcatgccac ggcgatcgct gtctggcagt
     6721 accgccagtt caatttgcag aattttgcga ataccggtcg cttccagatc gtggatcatg
     6781 cgcttaattt gctgacgcag caccaccgga taccacagtg cgtaagtacc agtggcgaaa
     6841 cgtttgtaac cttctgctat cccgctgacc accgcttgat agtcagtttt catttcatac
     6901 ggcgggtcga taaggattaa accacggcgg gaaaccggcg gcagtttggc cttaagctgc
     6961 tggaaaccgt cggctttttc gacacgcgca cggctatctt tctgaaattc agaacgcaac
     7021 aacgggtaat cgctcgggtg cagttcggtc agttgcaggc tgtcctgttc acgcagtagc
     7081 tggcgagcaa tcaacggcga accggggtag taacgcaact gaccgctacg gttgaagtgt
     7141 tttaccacat tgatgtacgc ctccagttct gcgggcaaat cgtcctgctg ccagatacgg
     7201 gcgatgcctt cgagatattc gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc
     7261 ccggcccctg cgtgggtgtc gagatagaga aacggtttat ctttctcttt cagcgactcg
     7321 atgatcaggc tctgaacggt atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg
     7381 tggcgataac tgagcatggg taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg
     7441 gcgcacaaaa gcgtatcagg acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac
     7501 gtttctcatt gaaattcact acacttaacc ccatgctaca cacattatgt aaagcgcctg
     7561 ttgagcgctt ccttaacctc tttaaccagg actgcgcgaa tgacgaatcc gttactgact
     7621 ccctttgaat tgcctccgtt ttctaaaatt ctcccggaac atgtcgttcc agccgtgact
     7681 caggcgctga acgattgccg cgaaaatgtg gagcgcgtag tagcgcaagg ggcaccgtac
     7741 acctgggaaa atctctgcca gccgttggcg gaagtggacg atgtgttggg gcgtatcttc
     7801 tccccggtca gccacctgaa ctcggtgaaa aatagcccgg aactgcgtga agcctacgag
     7861 caaaccctgc cgctgctgtc ggaatacagc acctgggtag ggcaacatga agggctgtat
     7921 aaagcgtacc gcgacctgcg cgatggcgat cattacgcca cgctgaacac ggcgcagaag
     7981 aaagcggttg ataacgccct gcgtgatttt gaactctctg gcataggtct gccgaaagag
     8041 aaacagcagc gttacggcga aattgctacc cgtctttctg aactgggcaa ccagtacagc
     8101 aacaacgtcc tcgatgcgac aatgggctgg accaaactcg ttaccgacga agcggaactg
     8161 gcggggatgc cagaaagcgc gctggctgcg gcaaaagccc aggctgaagc gaaagaactt
     8221 gaaggctacc tgctgacgct ggatatccca agctacctgc cggtaatgac ctactgcgac
     8281 aaccaggctc tgcgtgaaga gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc
     8341 ccgaacgccg gtaagtggga caacagcaag gtgatggaag agatcctcgc gctgcgtcac
     8401 gaactggcgc aactgctggg ctttgaaaac tacgccttca aatcacttgc cactaaaatg
     8461 gcagaaaacc cgcagcaggt gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg
     8521 caaggcgaaa aagagctggc acaactgcgc gcctttgcca aagccgaatt tggcgtcgat
     8581 gagttgcagc cgtgggatat cgcgtactac agcgaaaaac aaaaacagca cctctacagc
     8641 atcagtgacg aacagctgcg tccgtacttc ccggaaaaca aagcggttaa cggtttgttt
     8701 gaagtggtga aacgtattta cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg
     8761 cacccggatg tacgtttctt cgaactgtat gacgagaaca acgaactgcg cggtagcttc
     8821 tacctcgacc tgtatgcccg tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta
     8881 ggccagatgc gcaaagctga cggttctctg caaaaaccgg tcgcgtatct gacctgtaac
     8941 tttaaccgcc cggtaaatgg taaaccggcg ctgtttaccc atgacgaagt gatcaccctg
     9001 ttccacgagt tcggtcatgg tctgcatcat atgctgaccc gcatcgaaac cgccggtgta
     9061 tccgggatca gtggggtgcc gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac
     9121 tggtgctggg agcctgaggc gctggcgttt atctctggtc actacgaaac cggcgaaccg
     9181 ctgccgaaag agttgctgga taaaatgctg gcggcgaaga actaccaggc ggcgctgttt
     9241 attctgcgcc agctggagtt cggcctgttc gatttccgcc tccatgccga gttccgcccg
     9301 gatcagggag cgaaaatcct cgaaactctg gcagaaatca agaaactggt tgccgtagta
     9361 ccgtctccat cctggggccg tttcccgcac gctttcagcc atattttcgc cggtggttat
     9421 gccgcaggtt actacagcta cctgtgggct gacgtactgg cggcagatgc tttctcgcgc
     9481 tttgaggaag agggcatttt caaccgtgaa accgggcagt cgttcctcga caacattctg
     9541 agccgtggcg gttcagaaga gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg
     9601 cagctggatg cgatgctgga gcattacggc attaagggct gatcattcag tgaaaatctg
     9661 cttaattgat gaaacaggca ccggagacgg tgccttatct gttctggcgg cccgctgggg
     9721 gctggagcac gatgaagaca acctgatggc gctggtgtta acgccggaac atctggaatt
     9781 gcgcaagcgt gatgagccaa aacttggcgg catctttgtt gattttgttg gcggagcgat
     9841 ggcgcaccga cgtaaattcg gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat
     9901 taaaggcgat tatttgccgg atgtggtgga cgccaccgca gggctggggc gcgatgcctt
     9961 tgtgctggct tcagtcggtt gccgcgtgcg gatgctggag cgtaatccag tcgttgccgc
    10021 cttgctcgac gacggcctgg cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca
    10081 ggagcggttg cagttaattc acgcctccag cctgacggcg ctgactgata ttaccccgcg
    10141 cccgcaggtg gtttatctcg acccgatgtt cccgcataag cagaaaagcg cgctagtgaa
    10201 aaaagagatg cgtgtctttc agtcgctggt gggaccggat cttgatgccg atggattact
    10261 ggagcctgca cgcttgctgg cgaccaaacg cgtggtggta aagcgcccgg actacgcgcc
    10321 accgctggcg aatgtcgcca cgccaaacgc ggtagtcact aaagggcatc gctttgatat
    10381 ctatgcaggt acgccagtgt aaaactaaaa cagggtagcg ataacgctac cctgctttct
    10441 gcaagaataa ttaatggctt tccggcgtcg caatcatgcg tttcagccac ggcaccatca
    10501 acagcatcac tactgcaacg cccagcgtga ccagaccaat cttaccaaac acgttggtat
    10561 agacgggcaa cgtctcaagc gggtcggtaa tgttgtccgg caccgcggta aatgttgcca
    10621 catagccgcc cagcaagaac gcggcagcct gcgtcaggaa ccacatcccg agaataaagc
    10681 ccatcaaatg ctgcggcacc agggcagcaa tcatcgccag gccaagggcg ctaataaaca
    10741 gttcacctaa gctctggaat aagtacacca gcacgataaa ccatggcgat gtcagccctt
    10801 gtgcatccgc aaaccacatt cccgcagctg ccgccgtcaa aaagcccagt gagcacataa
    10861 acatgccgag agtaaatttc atcggcatcg agaggtcttt gcctttgtta cccagatgcg
    10921 tgtaaatgcc tgccagtatt gggctggcga gtaccaccca gaacgggtta agcgcctgga
    10981 agctgaccgg gttgatggaa aaaccgagaa tttcatgatg cacgttgttg atggcaaaga
    11041 agttcagcga tgttggcatc tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca
    11101 ggacaaaggc gacaaacatt ttattgcgcc cggttttatc cagcttgaat gcctgacgaa
    11161 agaagatgat ggtgacgacg atggagagaa caatcagcac cagattggcg acttctacgt
    11221 tgtgcatcag ccatgcgcat acgaagatca tcaccacgct gccaagtaat acgtacaaca
    11281 gtttgctgaa gctcatcggc ttgaagtcgg gttcagaacc aatgtctttc accattccac
    11341 gacaggcgat gtaaaccagt aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga
    11401 ctgaataacc gaatctatca gcgatcacag gggccagcga taacgctatc aacgagccga
    11461 tattgatcga catatagaac agggtgaatg cgccatcaag ccgcggatct ttcggcggat
    11521 agcacttcga aagcaagctg gctgggttag ctttaaacag gccgttaccg acagcgatag
    11581 tccctagggc gatgaaaatc aggtcaggct taagtagcga catgccggtc atgaagtagc
    11641 caatcgccag cacaagtgct ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc
    11701 cgacatagcc gccaatggaa atgaggccat agaccagcgc agcaaaagca ccaaaagtga
    11761 caaaagcctg ctcttgcgag aatccaagct gtttaacgaa gaaaaccgcc agtacgccct
    11821 gcacgccgta gtagccgaat cgctcccata actcgacaaa aaagatcatg aaaaatgggc
    11881 gaggttgctg cagcatcccc atgggtgttg ttgtattcat atttattaac cttccaatac
    11941 catcctgaaa agtatgaacg cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag
    12001 attcttaata ttgatgcggt gaatagatta atacactatt acaacagaaa ataaccagct
    12061 gatcacacta atgatggaaa atatgatggt gttaaatgtt gtattggcag gaggttttga
    12121 ttgcgtacgc agatcgtagg ccagataagg ggtttacgct gcatcaggta acaaggcgcg
    12181 ccaaaaagca aaacgcccgc atatgcgggc gttcaggaac acgtcgtgga gggatgatta
    12241 ttcttcttcg tcgcgcagcg gaacaatcag catatcaacg tgaacggtgt tgatcagctg
    12301 acgtgcggaa gacatcagtt tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc
    12361 catatcgtat ttcttgattg catcgaccag aacctggccc aggtcgccgc tgccgctcag
    12421 ggtttcagtg attgggtagc ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc
    12481 ttcagagatg cgtttctgca tatcacccag attcacatca ataagcccgg tgtataggtc
    12541 agagtagttt acatctacgt ggatcagaga aactttcgca ttgtaggggc gagccataga
    12601 gactgctttc tctaccagaa ctttgctttc cggggagaga tcgactgcga tgagaatgtg
    12661 tttataagcc atagtgttac tccttccata aagttgtcga tgactggcca gctagcgttt
    12721 cttgtgccgt tacgtcaccc gcgtcctgcg aactacgtgc gcggggctaa cccagccgta
    12781 aagattcagt gttaagacca tccttacctt atagcgaccc ggatgatccg tcaatccgcc
    12841 ttgcttaccc gttagataaa caaattttcg acatgaatat attgatagtg gttaaccttc
    12901 tggaaaaaaa acaacctgat ctcctacact atctatagag ccgctcggat gttggtcacc
    12961 acaaaagcgg tttctccagg acctggtgtt aactgattgg cagcgtatcg ggagcggtag
    13021 ccccggggtg gaactccgcg ggcaggtcgc cggggaggag gtatgataag caccgtcgca
    13081 ttattttggg ctttatgtgt cgtttgcatt gttaacatgg cgcgctattt ctcatcacta
    13141 cgcgcgttgt tagtggtact gcgtaactgc gatccattgc tctatcaata tgttgatgga
    13201 gggggctttt ttacctcaca tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc
    13261 tatgcccaac gttatcgcga tcatcacgat gatgaattta ttcgccgctg tgagcgggtg
    13321 cgtcggcagt ttattctgac cagcgcattg tgtggtctgg tggtggtcag cctgattgca
    13381 ttgatgattt ggcattaagc acttcagcaa taataacgca taaaaaaagc gggccagtaa
    13441 actgacccgc tcgttttggt gtgtacgatt acaggaactg caaggagagc cagtacagtc
    13501 cgccggaaag cagtaccgca gccggaaggg taaacaccca ggccatcaga atgctggtca
    13561 cggttttacg ctgtaagccg ccaccatcta ccaccatcgt ccccgcgaca gaagaggaga
    13621 gtacgtgagt agtggaaacc ggcatcccgg tataactcgc caggccgata gacactgccg
    13681 ccgtcatctg ggcagacatc ccctgagcgt aggtcatgcc tttcttaccg attttctcac
    13741 cgatagtcgt tgccacacgg cgccagccaa tcatcgtacc gatacctaac gccagcgcga
    13801 ccgccatgat gatccacacc ggtgcatact cgatggtgct aagcatgtcg gacttcagtt
    13861 tcttcaacag gcgctgatcg tcagcactca cgccaggcat cttcaccact ttgtcgatag
    13921 tgtcagaaac gcacagcata atgcggcgca tctggctacg ttgatcaagc gacagcttgt
    13981 cgtagctttc cacatcggtg gtcaacatac ctttcaggcg gttgagcgcg ttaatggtat
    14041 tcgacggatg gcagtggaac tccgcaggtt gcgttgcgcc agcttccgga gccggtacta
    14101 actgatcagc accggtagcc tgtttgagca gcgcaggatg ctgctcaaag taagcttcga
    14161 cgttgttgat ggcatcacgg gtacgggtga tttcgtagcc agtggcattc atgttcacca
    14221 cgaagcctgc tggcgcgacg ccaatcaata ccaacataac cagaccaatg cctttctgac
    14281 catcgttcgc gccgtgcgaa aacgccacgc cgatagcgga aaggatcagc gcgatacgcg
    14341 tccagaacgg cggctttttc ttgccgtctt tcttttcacg ctccgctggg gtcaggtgga
    14401 tacgggcgcg tttcttggtg ccgctccagt agcgacgcag caagaaaatc agaccgccag
    14461 caaacaccag gccgacaata ggggaaacga tcagagaacc gaaaatactt aatactttcg
    14521 ggatattgag tgcatccacc actgacgtcc cggtcatcaa cgcattggtt aaaccaatcc
    14581 cgatgatcgc gccaatcagc gtatgagagc tggatgcagg taaaccaaag taccaggtac
    14641 ccaggttcca gataatcgcc gccagcaaca tagagaacac catggcaagg ccatgagacg
    14701 atcccatatt aagcagcaga tccgtcggca gcatatgcac aatggcatag gcaacactca
    14761 gaccacccag caaaacaccc aaaaagttga acaccgccgc cataaccacg gcgagctgag
    14821 aacgcatcgc gcgggtatag ataacggttg ccacggcgtt ggctgtgtca tggaaaccat
    14881 tgatggcttc gtagaacagc acaaaagcca gtgcaagcaa taataacagc ccggtatgca
    14941 aatccaggcc agcaaacaaa tgtagcatag gacgttacgc cattttgagg acatgaacgc
    15001 ggcgcattat cagtgacttt cgcggcgcgg gcaaagtgaa atatagactt tttttgattt
    15061 gcctcctgta tggatttcac tcaaaaaata attatcttat ataattcagg caaatacttc
    15121 cttttagtaa tattgatgct ggtgcgacca ctgaggaatc tttacaattc acgcccgttt
    15181 tttctaagag gagcgcaacg tggaaaggtt tgatgccatt attataggcg ctggtgcggc
    15241 gggtatgttc tgttctgcgc tggcaggtca ggcaggacgc cgggttctgc tgatcgataa
    15301 tggtaaaaaa ccagggcgca aaatccttat gtctggcggt gggcgctgca actttaccaa
    15361 cctttatgtc gaaccaggcg cttatctgag ccagaatccg catttttgta agtctgcact
    15421 cgcacgtttt acccagtggg atttcattga tctggtcaat aaacacggca tcgcatggca
    15481 cgagaaaacg ttagggcaac tcttctgcga tgactccgcg cagcagattg tcgacatgct
    15541 ggtggatgag tgcgagaagg gcaatgtgac cttcagattg cgtagcgaag tgctgagtgt
    15601 ggcgaaggat gaaacaggct tcacgcttga tctaaacggc atgactgtcg gttgcgaaaa
    15661 gctggtcatc gcgaccggtg ggctgtcaat gccggggctg ggcgcatcgc cgtttggtta
    15721 taagattgcc gaacaatttg gcctcaacgt gctgccgacc cgcgcgggtc tggtgccgtt
    15781 cactctgcat aaaccgttgc tcgaagagtt acaggtgctg gcgggcgtgg cggtgccttc
    15841 cgtgattacc gctgaaaacg gcaccgtttt ccgtgagaac ttactcttca cccatcgcgg
    15901 cttgtctgga ccggcggtgt tgcagatttc cagctactgg caaccgggtg aatttgtcag
    15961 tatcaatctg ctaccggatg tggacctcga aaccttcctg aatgagcagc gtaacgcaca
    16021 tccgaatcag agcctgaaaa acacactggc ggttcatcta ccgaagcggt tggttgaacg
    16081 cttacagcaa ctcgggcaaa tcccggatgt ttcgctaaaa cagctcaacg tgcgtgacca
    16141 acaggcactg attagcacat tgaccgactg gcgcgtacaa cccaacggca ctgaaggcta
    16201 tcgcactgcc gaagtgacgc tcggcggcgt ggacaccaac gaactctctt cacggacgat
    16261 ggaagcgcgc aaagtgcctg ggctgtactt catcggcgaa gtgatggacg tcaccggctg
    16321 gctggggggc tataacttcc agtgggcgtg gtcgagtgca tgggcttgtg cgcaggattt
    16381 gattgcagcg aagtcgtcct gacctgttac ctaaagatga aaattattag agccgcatta
    16441 aaaatgagct aattttgata gtggctatct tgtgattatt ttctaatgag cccgtgaact
    16501 gaaaccctcc aggcttaata taaggtggaa ggaaaggtga ttgaaaactc acccagtggc
    16561 cagatcttta taaaatatga acaggctgaa aaagacagta actttgttgt ttttttatcc
    16621 ttaaccacta tgcattaatg ctgcgttatt tcatgatgcc taagaaaaac cagcgttacg
    16681 caaatggtca acgctggttt tatccggtac gttgcaatta ttttttagca gaacccgctt
    16741 ctaatattga aacgattgag aacaacgtaa agcataccgc tcccaaaccg accagaactc
    16801 gcgaaggaat aaaatatccc atgtcggtct gcgccatttc cgcaagaaat gatgccagga
    16861 aaaggcaaaa caggcaggtg aagacgggta tcatcggtat tctgttggct aacgaacagg
    16921 tacgtctcca gaccagtgcc agtagccaga cttttgagaa tatgctgtaa cagatcattc
    16981 cgaggcaaat aagaataatg ccgggagcca gtcgggcgct tgcatcggaa ctgactaaga
    17041 cgtatatccc ctgcagtacc gtgattgagc cgagaaaaat aacccaataa caccacagcc
    17101 agtgttcttt agttgaaaac gtattacgtg tttgatggac aattgtggca acaaggccaa
    17161 ttagacaggc acagattgcg gttaacccaa gcaatacgtg acccgcgaca taatgcggag
    17221 ttatgtcagc actacgtaac agcgtaatag accaaatgaa accgagcagg gtgagtaaaa
    17281 cgggaacggc aatgaggcaa ttaccgatta atgaagaata agcctgtacc ggcgttccat
    17341 cgctcttgct ccccgctgca tttttgggaa tgagcagaaa gtgaccggat gacgctgcca
    17401 ccgtcgatac acaggcggca atcataccaa cgccgaaaat aacatggccg gcgacaaact
    17461 cgtctgccat cacatcattg cctgctaaca gtgcccaacc ccatatcatg gtgataattg
    17521 accccgcata gccaataatg gggaacaatg tattgtaaaa cgtattaacg ccgcgcgtga
    17581 gctgcgaaat gataataaat gcggtagtga ataatgccag gcatatggcc gccagagaaa
    17641 tcagcacatg acccgcgacg aaatactcgc tttgccctga actttccagc acaaagccac
    17701 ctaacgcgat gcaaattaat cccataatca aagggattaa tttgaaaagc cacccgatat
    17761 atatgttcac tttaaactcc tcaggtatcc ctgctaataa tgaaatgtgc attaatgata
    17821 atttcagcct gttaatataa cgacgaga
//
LOCUS       DAZKIV010000096        17846 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZKIV010000096 DAZKIV010000000
VERSION     DAZKIV010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17846
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.096"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..778
                     /locus_tag="ACHOT6_003109"
     CDS             71..778
                     /locus_tag="ACHOT6_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFP9948284.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            775..2226
                     /gene="djlC"
                     /locus_tag="ACHOT6_003110"
     CDS             775..2226
                     /gene="djlC"
                     /locus_tag="ACHOT6_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFP9948285.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(2286..3956)
                     /gene="hscC"
                     /locus_tag="ACHOT6_003111"
     CDS             complement(2286..3956)
                     /gene="hscC"
                     /locus_tag="ACHOT6_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFP9948286.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELGALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLSLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(4040..4975)
                     /gene="rihA"
                     /locus_tag="ACHOT6_003112"
     CDS             complement(4040..4975)
                     /gene="rihA"
                     /locus_tag="ACHOT6_003112"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFP9948287.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(5093..5818)
                     /gene="gltL"
                     /locus_tag="ACHOT6_003113"
     CDS             complement(5093..5818)
                     /gene="gltL"
                     /locus_tag="ACHOT6_003113"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFP9948288.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(5818..6492)
                     /gene="gltK"
                     /locus_tag="ACHOT6_003114"
     CDS             complement(5818..6492)
                     /gene="gltK"
                     /locus_tag="ACHOT6_003114"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFP9948289.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(6492..7232)
                     /gene="gltJ"
                     /locus_tag="ACHOT6_003115"
     CDS             complement(6492..7232)
                     /gene="gltJ"
                     /locus_tag="ACHOT6_003115"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFP9948290.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLT
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(7402..8310)
                     /gene="gltI"
                     /locus_tag="ACHOT6_003116"
     CDS             complement(7402..8310)
                     /gene="gltI"
                     /locus_tag="ACHOT6_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFP9948291.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNCVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            8765..10642
                     /locus_tag="ACHOT6_003117"
     CDS             8765..10642
                     /locus_tag="ACHOT6_003117"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFP9948292.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(10720..12258)
                     /gene="lnt"
                     /locus_tag="ACHOT6_003118"
     CDS             complement(10720..12258)
                     /gene="lnt"
                     /locus_tag="ACHOT6_003118"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFP9948293.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(12283..13161)
                     /gene="corC"
                     /locus_tag="ACHOT6_003119"
     CDS             complement(12283..13161)
                     /gene="corC"
                     /locus_tag="ACHOT6_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFP9948294.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSEAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(13251..13718)
                     /gene="ybeY"
                     /locus_tag="ACHOT6_003120"
     CDS             complement(13251..13718)
                     /gene="ybeY"
                     /locus_tag="ACHOT6_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFP9948295.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(13715..14755)
                     /locus_tag="ACHOT6_003121"
     CDS             complement(13715..14755)
                     /locus_tag="ACHOT6_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFP9948296.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(14908..16332)
                     /gene="miaB"
                     /locus_tag="ACHOT6_003122"
     CDS             complement(14908..16332)
                     /gene="miaB"
                     /locus_tag="ACHOT6_003122"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFP9948297.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            16478..17653
                     /gene="ubiF"
                     /locus_tag="ACHOT6_003123"
     CDS             16478..17653
                     /gene="ubiF"
                     /locus_tag="ACHOT6_003123"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFP9948298.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGNSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARNYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLSFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            complement(17656..>17846)
                     /locus_tag="ACHOT6_003124"
     CDS             complement(17656..>17846)
                     /locus_tag="ACHOT6_003124"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948299.1"
                     /translation="SGIPRFESSYPSHIKKARFGELFAFLRSFNVECDVDTSYPSMSD
                     ATLPRLIRPTVGTHPAML"
BASE COUNT         4357 a         4473 c         4641 g         4375 t
ORIGIN      
        1 gaaagcgtca agtttatact catcaataat aaaaagtagt cagttaatgc acttaaggga
       61 ttattaacaa atgaataagg aagaacaata tcttcttttt gcgctttctg cgcccatgga
      121 aatacttaat cagggttgca aacccgcgca tgactcgcca aaaatgtata ccggtataaa
      181 agaatttgat ctctcatcat cctggggaat aaataatcgt gatgatttaa ttcagactat
      241 ttatcagatg actgatgatg gtcatgccaa tgatttagct ggcttatatc tgacatggca
      301 tcgctcttcc cctgaagagt ggaaagcatt aattgctggt ggctcggaga gagggttgat
      361 ttatacccaa ttcgttgccc aaaccgctat gtgctgtgga gaagggggaa tcaaagcctg
      421 ggattatgtt cgaatggggt ttctgtcacg ggtcggtgtg ctcaacaact ggctgacgga
      481 agaagagagt ttatggctgc aatcgcgcgt ctatgtacga gctcaccatt actatcacag
      541 ctggatgcac tatttttccg cctattcgtt aggacgtctc tactggcaat cttctcagtg
      601 cgaagacaac acttcgctac gcgaggcgct aaccctgtac aaatacgata gcgccgggag
      661 tcggatgttc gaagagctgg cagcgggaag cgatcgtttc tatgccacgc ttccctggca
      721 gccattaact gtccagcctg aatgcccggt aacgcttaag gatgtgagcg acctatgaaa
      781 acatgttggc aaatcctcga aattgaaagc acgacgcaaa tagacattat ccgccaggct
      841 tatcttgctc gcttaccgtt atgtcatccc gaaaccgatc cgcaggggtt taaagcatta
      901 cgccaggcct atgaagaggc gctgcgactg gcggtaaatc ctgtcgagga agcagatgat
      961 gaagaaaaag atgccgccgc tgaacatgaa atactgcgtg cattcaggac attactggat
     1021 tcagaaagtg atcgttttca gccctccgcc tggcagaaat ttattcagca attaaatacc
     1081 tggaacatgg aggatgtcga tcaattacgc tggccgctgt gtgcaatcgc tatagaagcg
     1141 cgatatcttt cattaaattg tgcttctttg ctggcagagc gtttgaactg gcattcgttt
     1201 aatgacagcg aaggaatgga tgaggaagaa agggaggctt ttcttgaggc cattcaggct
     1261 ggtgattgtt tcgatttcct tagccttctg gaatatcccg ttgcgttgca gaaccagact
     1321 gttgagtatt acttcgcgct ggaacgttgc tgccgttacc atcctgacta cgtcactgcg
     1381 tttttggcga tggaaggtcc gtggttcatt cctgatgatg caaagttaca tcgcaaactg
     1441 ttgcgctggt acagctcggt gcaaacaggt atggcggaac tcattcctgt agccaaacag
     1501 tggcaaatgg aagaaccaga aagcgaagat gcccgatatt acttgtgtgc tcaaaggttg
     1561 tactgcggcg agggggaaag cctgcttgcc gatctctgcg cgtaccggga aagttaccca
     1621 tctacacaag ctgataattt gttgttgcag tggagcaagc gtcattgccc ggattatttc
     1681 gcgttattag tgatggttat cgaagcgcgg agcatggtag atgcgcaagg tcaaccgctg
     1741 aaatatgttc ctggtgagag cgcccggacg cggctgttat gggcggaaat tttacatagc
     1801 ggaaaattat cgccgttagg tcaatcgttt attgagtcgt tattcttcaa acgcaaagca
     1861 tgggcgtggt ggaaatcgag agtcggtagc gagacagaag aagattcacc gttactggat
     1921 ttgtatcggg tagcggaaca ggtagtactt gaagcgtttc cgaaacaaga gatgctggcc
     1981 cgtcttaata caaggctgga aggcggagat gctcatccat tagaggccat tatcacccgg
     2041 atgctgttga cgaaagtaaa actcgagccg gaggatgaag atgtcgatga gccaacacct
     2101 gaaaaccatg aagaaaaaaa tgatgagggt gaaaaaccac agagcattac cagcattatc
     2161 aaaatcagtt taacggtgct ggttataggt tatgctctcg gcaaaatcgc gatgttgttt
     2221 agttgacgat gtgttttttg cgccgatgcc ggatgcagca tttacgccgc atccggcagc
     2281 cgtgattatg aggtctcaat ggctaaataa acgctcgccc gccgcctgac ctcatcgacg
     2341 cgggccatat cgtttgactg caacgccgca tcaaaatcgg tgatcacccg gccaatctct
     2401 tctcgctcgt cacccagcgc ccgcgcccac aactcttcca gtttggcctt aaaggtgcgg
     2461 ttgatgagca tatcgcgcgg ataaattttc aatgcggata accgcgtccg actctcttca
     2521 atctgctgcg cgctcaatgt taccgggcta tgattaatga ctctggactt aacgctgcca
     2581 tcttccagaa gcacgtcaac ttcaagcaag ccgttgatat cataactaaa gcgaatatca
     2641 atcgactgat aagccccggt tttcttcaac ggcacatcga aggattcaac cagaatgtta
     2701 tttttaactt tgtggttttc tccctgatag acgttaaccg taattgaatc ctgttccggg
     2761 tgcatggtcg agtaagtttc tacacgcgac acgggtactg tggtgtttcg ttcaataatt
     2821 ggcgagaaaa tgccggaaac gccctggcgg ttaacttcaa cgcccaacga gtaagggcaa
     2881 atatcagtga ggatcacctc ttcaatatct tcactgcgta agcggcaggc ggcctggatt
     2941 gctgcgccca gcgcgacaat ggtgctcggg tcgtaacttt gatacggtaa tttgccaaac
     3001 agacgcacgg cgattcgctg caccagcggc atttgtgacg cgccgccaac cagcaccaga
     3061 ctgtcgattt gactcggctt cagacgtgca tcgcgcaacg cctgttcaat cggcactcgc
     3121 aagcgattga gcagcgacaa ccacaaatct tccagttcgt tctcgtaaaa ttcgcactcc
     3181 cgcgtttcat cctggtactg ccagcgaatg tgcaatggcg attgattgct acattttgcc
     3241 gcttccacac aggcgtacaa ggctcccagt tcactctcgt taagcgtggt cctggcgaca
     3301 tccgcgcgtt ttaaaacctc atcgaccagc atatgggtaa aatcttcgcc accaagaaag
     3361 ttgtcgccag cggaggcgtg aacttcaatc accggcgtgg cgtactcaag caccgtaacg
     3421 tcaaacgtgc cgccaccgag atcaaacacc agcgaacggg tattttgttg ggtatgcagg
     3481 ccatacgcca tcgcagctgc tgtgggttca ttaattaagc gtaccgcatt taacccggct
     3541 aactccgctg ctaaacgggt atgcttgcgt tgttcatcgc tgaaataagc cggaacggag
     3601 atcaccacat ctttaatcgg acgttgcaga aattcttcgg catcttcttt taatgagcgt
     3661 aataccaaag aggacagttc tggcgcgtta aatgtgtcgc tgcctaaccg ccagttggta
     3721 ttactgccca ttgcacgttt aaataacgct gccgttttat ccggatgcga agtacgccgt
     3781 gatacagccg gttttccgac taaaatatga ttattttcat ccatgctaat tatggatggt
     3841 gttaaatatt caccgaactt atttggaatt aattgcgcgg caccgtcttt ccagacggca
     3901 attaaactat tggtagtgcc gagatcgata ccaatggcga gttctgcatt atccattgca
     3961 tttatatctt gaaagaaaaa gagataaaca gattaagacc acccaggtaa cgcgtggtga
     4021 tcttaatcaa tgacgtgtgt taagcgtaaa atttcagacg atcagccagt aaatcaacaa
     4081 agccctgacg atcaacatcg accattacag tggcattcgg tttattgcca gtcagataat
     4141 aataatcaac aaccgtcata ccctggctat atttcccctg tgtttccacg ccaacccagc
     4201 gctcaacagt ggtaaacaac tcgggtttca acagccaggc gatggtgcat gggtcatgca
     4261 gcggtgcacc gacaaagccc catttttcgt ctttatgata ttcgaggaag aaatccagca
     4321 gttcggcaac aatggttgaa acagggttgc caatcgcgcg gaaacgctcg gtgtcttcaa
     4381 cgtggatttg tgctttgtga gtaacatcca gaccggccat caccactggg atccctgact
     4441 ggaagacaat ttctgccgct tccgggtcaa cgtaaatatt aaattcagcc gcaggcgtcc
     4501 agttaccaag ccccattgcg ccgcccataa tcacgatacg ggcaattttg ctatgcagtt
     4561 ccggatggct attgagcagc aaggcaacgt tagtttgcgg tccggtagac acaatcgtga
     4621 caggttccgc actttcgcgc agcgttttcg ccatcagctc taccgccgta cagttttgcg
     4681 gtgcgaacgt cggttccggt aatgccgggc catccagacc gctttcgccg tgcacattgt
     4741 ccgcgataat caactcacgc attaacggtt ttaccgcgcc gcctgctacc ggaatatcgg
     4801 tgcgattaag caaggtcagc atacgaagaa cattgcgtaa ggttttttct ggtgtctggt
     4861 ttccggcgga agacgtaatt gctttgacat caagctctgg tgaggcgagg gcgagaacta
     4921 ttgcgatagc gtcgtcatga cctgggtcgc aatctaacag aattggcagt gccattgttg
     4981 ctccttgttg tgtgcttctt tgcgacaagg gtaacgccag gatgtaacag atacgagggg
     5041 cgaaacgata aagcgtgaga tggcgcgcac ttgggtatgc gcgccagagt gattaatgca
     5101 ggattttcgc gaggaagtct ttcgcgcggt ccgatttcgg atcatcgaag aaagcgtctt
     5161 tcggcgagtc ttcgacaatt ttaccctcgt ccataaagat cacccgattc gccactttac
     5221 gggcaaaacc catttcgtgg gtcaccacca tcatggtcat tccttcgttt gccagttcca
     5281 ccatcacgtc cagcacttcg ttgatcatct ccggatccag cgccgatgtc ggttcgtcga
     5341 acagcatcgc aataggatcc atacacaacg cgcgagcgat tgccacacgc tgctgctgac
     5401 cgccggaaag ctgcgccgga aatttattgg catgtgcgga aagtcccacg cgctccagca
     5461 atttcaatgc tttttcacga gcgggcgctt tatcgcgttt aagcactttc acctgcgcca
     5521 gggtcaggtt ttcgataatc gacagatgag ggaacagctc gaaatgctgg aataccatcc
     5581 cgacgcggga acgcagcttt gccagatcgg ttttcttgtc attaaccacg ataccatcga
     5641 cggtgatttc accttgctgc actggttcga ggccgttgac ggttttaatc agcgttgatt
     5701 tgccggaacc agacgggccg caaaccacca ccacttcgcc ttttttcact tcggttgagc
     5761 agtcggtcag cacctgaaag tgaccatacc attttgaaac atttttcagg gtaatcatta
     5821 tgctgtcctt cttttcaagt agctgaccaa caacgacgcg ctaagactaa taacgaaata
     5881 aacaaatccg gcaaacagga tcatctcaac ctgcgtacca tcacgctcac caatggttga
     5941 ggcggtacgg aagaaatcgg ccagagataa cacatacacc agtgaggtat cctggaacag
     6001 tacgatgccc tgagtgagca gaagcggcac catcgcgcgg aacgcctgcg gcagaataat
     6061 cagtttcatc gactgccagt gagtcattcc caacgccagc gcggcgctcg actgaccacg
     6121 agaaatactt tgaataccag cacggataat ctctgaatag taggccgctt caaacatcga
     6181 aaacgccacc atcgccgaaa ttaaacggat atcatttttt ggtgataatc ccagcacgtt
     6241 ttgcagaaaa cctggcacga tcaggtaaaa ccacagcaaa accataacta aaggaatcga
     6301 gcggaatacg ttaacgtagg ctttggcaaa ccacgctacg ggcgcaaagc tggacaagcg
     6361 catcaccgcc agcatggtgc cccacaaaat accaatcacg actgccgtga cggtgatttt
     6421 cagcgtgatc accagcccgt cgagcagata tggcagggaa gggacaatgg aactccagtc
     6481 aaactcgtac attatttgcc ccccatgttg ccaggcaggc gaactttacg ttcaaccagc
     6541 gtcattacca gcatgataac cgcgttaatc aacacatacg tcagcgtaat agcggtaaac
     6601 gactcccagg cgtgggctga gtaatcaagc aatttacccg cctgcgccgc catatccacc
     6661 agaccaatag tcgaggcgat ggcggagttt ttcaccaggt tcatcatctc ggaagtcatc
     6721 ggcgggacga taacgcgata agcattaggc agcagtacgt atcgataagc ctgcggtagc
     6781 gtcaggccca tcgccagcgc ggcatttttt tgcccacgcg gcaacgactg aatggcggcg
     6841 cgtacctgtt cgcaaacacg ggcggcggta aacagcccca ggcagagcat ggatgaaagg
     6901 aaaaactgaa tattgggatc cagctcggcc ttaaaccaca tgccgatttt ctccggcaac
     6961 agctccggga tcaccagata ccaggtaaag aattgcacaa tcaacggcac gttgcggaac
     7021 agttcgacat acaacgtacc cagaccggag agaaaacggt ttggaacggt acgtaaaatg
     7081 ccgaaaaatg aaccgacgag gaaagcgata atccaggcgc agatcgacaa agcgatcgtc
     7141 acctgaaaac cactccagat ccaaccaaga taggtggtgt tgccgaacgg ggcttgttgt
     7201 aaaaaaatac cccagttcca gtctatagac ataaatctac tccagaaaaa agagggtagc
     7261 agcgttaact gctaccctcg aagattgtta cccagcgtat tgcggttttc aggcccgatg
     7321 gggaacgacc atcaggcgta tagtctgtcc gtgctacgta acaatcgaga gggctggaat
     7381 ttccgcccct ggttcttgta attagttcag tgccttgtca ttcggttctt tgaacagtgc
     7441 tttcatttcg tctgacagtt cgaaattcat gttcaggttt ttcggcggaa ttggattttt
     7501 gaaccattta tcaaaccatt tttccgcttc accggaggtc tgcacctgag cgatggtgtc
     7561 atccatcagc tttttgaact gcggatcgtc tttacgcaac atgcaaccgt aggcttcctg
     7621 agactgcggc ttgccgacga tttcccagtt gtctggtttc ttcgctttcg cacgttcacc
     7681 ggccagcagg gcatcatcca tcataaaggc aacggcacga ccgctttcca gggtgcggaa
     7741 agagtcaccg tgatctttgg cgctgatgat gcgcatattc attttttgct cttcattcag
     7801 tttgttgagc aaaacttcag aggtagtgcc ggaagtgacg actacggctt tgtctttcag
     7861 gtcggcaaaa tctttgatat cgccgccctt tttggtcagc aggcgcgtac cgaccacgaa
     7921 aatagtatca gagaaagccg cctgtttttg gcgttcgacg ttgttggtgg tagaaccaca
     7981 ttcaaaatcg aaagtgccgt tttgcagcag tggaatacgg ttttgtgagg taatcggaat
     8041 cagttttacc tgcaagtccg gtttgttgag tttctttttc actgcttcaa caatggcgtt
     8101 ggagtaatcc tgcgagtaac ccaccacttt ttgctgattg tcgtaataag agaaaggtac
     8161 tgaagattca cggtgaccga cgacaatcac acagtttttg gcgattttgt ccagcgtact
     8221 gcctgctgcc ggggcggcgt catctgcctg tgccagtcct gcggaaagcg ccagggcgag
     8281 gattgctgtg gcaggtttac gtaattgcat atccaactcc tttatcttct gcgttaagaa
     8341 cgcatggata cccgttgtga gtgtttgtgt tgttatcgtc tgcaacttta ttgtgcagtg
     8401 ttgtgcctgt taggtaacat ttagtttggc taaatgtaaa gatattgctg ttttattgtt
     8461 tgtttttgcg agatgcgccg caccattccg aagcaaaatt cttaaaccgc acctttttgg
     8521 tgctaccgct gatttgtggt ggtgcaacct ggttgcgtcg cggctgattt catcgcttac
     8581 acagtaaata aagcaactta catgccaaaa aatacttagg ggcgaattaa ttacgattgt
     8641 aattttaggc tgaaaaagtc atatttcgga cagaattatc ttttcggcat gggacgtttg
     8701 ccgggatgat aactgcacac gggaaaaatg ctttttacct gtcttttcaa ggaagaaaca
     8761 ccttatgtct gcatcttcgg ttaagccgtt aaatgttcaa ttacccgcaa taacccttat
     8821 cctttttgca ctctgtattg ggatattttg ttatctcgca caatggatga gttatgaaga
     8881 ggtcgatcaa tccgcactta tccatctcgg tgcaaacgtt gcgcctctta ctttgtcggg
     8941 tgaaccctgg cgcttattga gcagtatctt tctgcacagt agtgtttctc atctgctgat
     9001 gaatatgttt gcattcctgg tcgtgggagg cgtggcggaa cagattctgg ggaaatggcg
     9061 actcctgatt acctggttat tctccggcgt ctttggtggg ctcatcagcg cctgttatgc
     9121 gttacgcgaa agtgagcaga tagtcatcag cgttggggca tccggggcga ttctgggaat
     9181 agctggtgca gcgatagcaa cacagtttgc ttcaggtacg ggcacatacc ataaaaacca
     9241 gcggcgagta tttcctctgt tgggtatggt ggcgctgaca ctgttgtacg gtgcccggca
     9301 aacaggaata gataacgctt gccacattgg cggcctgatt gcgggtggcg cgttgggttg
     9361 gctgagcgcg cgtttatctg ggcaaaaccg actcgttacg gaaggcggga ttattgttgc
     9421 gggcagtctt cttctgaccg gggctatctg gcttgcgcag cagcagatgg atgagtcagt
     9481 tttacaagtc aggcaaagcc tgcgtgaagc gttttatcca caggagattg aacaagagcg
     9541 acggcaaaaa aagcagcagt tagcggagga acgcaacgcc ctcagggaaa cattatccgc
     9601 tccggtaagt cgtgaacagg ccagtggtga tttgctcgct gagattgccg atatccatga
     9661 tatggcgatc agtcgggatg gtaatatgtt gtatgccgca attgaaaaca cgaacagcat
     9721 tgttgttttc gacctcggac aaaagaaaat cctgcataca tttacagcgc ccatagcgaa
     9781 agaaaagtca gtcaaacatt gtggtggctg taaagatcag ggcgtcagat cgctggcatt
     9841 aagcccggat gaaaagttga tttatgcgac ttcatttgaa gcgaatgcgt tatcggtcat
     9901 taacgtggcg acaggggaga ttattcagtc gattaccacc ggtgcacatc ctgacagcct
     9961 tatcctctcg cgtgatggca caaaagcctg ggtgatgaat cgcaccagta atagtgtgtc
    10021 agcgattgat ctggtgactt atcagcatgt ggcggatatc ccgctggaga aatacgacgg
    10081 gacggggacg agcggtaaac caggcgcttg ggttatggca ctttccccgg atgaaagaac
    10141 attactggtt ccgggagcag gcagaggtaa catcgtgcgg atcaatacca tcacgcatca
    10201 gaaagaagac tttcccgcag gtgatgcgcg tggaacgata tcggcgatgc gttttcgacc
    10261 tgaaaacggc gaggttattt ttgccgatag tcaggggatt tcacgtataa gcgtagggga
    10321 tcaacaagcc agcattatga cgcaatggtg tagcaggagc gtttattccg ttgagggtat
    10381 tagcccggac ggtcaatatt tagcgttggt gtcatatggc ctgcaaggtt atgtcatcct
    10441 gctcaatatt aatgccgggc agattattgg cgtttatcct gccagctacg ttaatcacct
    10501 tcgtttttca gcggatggta gaaaaatatt tgtcatggcg aagaacgggt tgatccaaat
    10561 ggacaggacg cgctcgcttg atccgcaggc aattattcgt catccccaat atggcaatgt
    10621 ggcttgtatc cctgaaccgt aagaaaaagg ggcatgataa ccatgcccca aagctcaatg
    10681 agaaagtacg ttttgctcat gcaggataca tcaggcacat caacgtttac gtgctcgcag
    10741 actcatcagc acagcggcaa aaccaaacag cgccgtcagc acccacagcg gccagttgcc
    10801 ggtacgtgca tacggtgtga gtccggtggt cggtgtcaca ttagtggtta acacctcgcg
    10861 ggtgaactgc gggatcatcg cctgaatctc accctgcggg ccaatcaccg ccgtaatgcc
    10921 gttgttggtg ctgcgcaaca gtgggcgcgc cagctccagc gcacgcattc gcgccatctg
    10981 gaagtgttgc catggaccaa tagatttacc aaaccacgca tcgttggaga tagtcagcag
    11041 atagtcggta tccgggcgga agttatcgcg cacttgctcg ccgagaatga tctcgtagca
    11101 aatagccgca gtaagctcaa taccatttac cgacagcggc ggctggatat atggcccacg
    11161 gctgaacgac gacatcggca gatcaaagaa cggtgctaac ggacgcagaa tcgactccag
    11221 cgggacaaac tcgccaaacg gcaccagatg gtttttgtta tagcgatcgg ctgattcgta
    11281 gctgtacggt gcacctttac ccagtgtgat gatggtgttg taggtatcgt agcggttctg
    11341 cttattgaga cgcgcgtcga caatcccggt taccagcgag ctacctttat cgcgcaactc
    11401 accgtccagt gctttgagga acggttgctg attaatttcc agatcggtta tcgccgactc
    11461 cggccagata atcaacgatg atttgcccat cagcggagcc gttgcgttgt agtaaatctt
    11521 cagcgtatta agaagctgat ctccatccca tttcagtgat tgcggaatat cgccctgaac
    11581 catcgaaacc tgaatggttt tctccggttg tggggtaaac cattggatgt aacgcagcgg
    11641 gaagggtagg gcaaacagca cgacggccac caccagcgga cgccagttgc gtttgaccaa
    11701 cgccagtgcc agcaggccac taaccatcat cagcaggaag ttaatggctt ccacgcccat
    11761 tatcggtgcc agccctttta acgggccatc aatctggcta tagccgaact gtaaccacgg
    11821 gaagcctgtc agtacccaac cgcgcagaaa ctcggtcact tgccagagag aaggggcggc
    11881 aatcgccacg cgcagccagg tggttttcgg ccacagacgc gacagcacgc cagcaaacag
    11941 tccggtatac agcgacaaat acgccgccag cagcaccacc aggaagatgt taaccgggcc
    12001 aggcatcccg ccaaaggtcg cgatgctgac atagacccag ttaataccgc tgccaaagag
    12061 gccaaatccc cagcaaaagc caatagcggc agactggagt ggacggcggt taaaggtcaa
    12121 cgcctgaagc cccatcaacg aaataatcgc cgcaggccag acgtcgtaag gagagaaggc
    12181 cagcgttccg caggcaccga ataataacgc cagcagcagg cgaatgcgct ggcgttcaat
    12241 taatgaggca aaagccatgt agttatctat ccagtttcgg ttttattcat ccagcttcgg
    12301 ctggggtgag tcatccggga ttttgacatg aacctgaata atacgccgac tgtcggccat
    12361 cgccactttg aactggtaac cgtcgatgtc gatagtttcg ccacgcgccg gaagatgccc
    12421 aaatgcctgc atcaccagac caccgatagt gtcaacctct tcatcgctaa agtgggtgcc
    12481 gaacgcttcg ttgaagtctt caatggaagc cagtgcgcgc acggtccagg tatgacgact
    12541 cagctgacgg aagtcgatat catcttcttc gtcatactcg tcttcaatct caccaacaat
    12601 tagttccagg atgtcttcaa tggtcaccag accggaaacc ccaccgaatt cgtcaataac
    12661 gatcgccatg tggtaacgct gagagcgaaa ctctttcagc atccggtcta cgcgcttact
    12721 ttcaggaacg acaaccgcct gacgtaacac tttgtccatg ctgaaggctt cagcttcgct
    12781 gcgcataaac ggcagcaagt ctttcgccat cagaatccct tcaatgtgat ctttgtcttc
    12841 gctaatcacc gggaaacgtg agtgggcgga ctcgatgatg acatcaaggc attcgtccag
    12901 cgtctggttg cgtttcaggg taatcatctg ggagcggggg atcatgatgt cgcgaacgcg
    12961 ttggtctgcg atgtccatca ccccttcgag catatcgcgc gtatcttcgt cgataaggtc
    13021 gttctgcccg gaatcacgga tcagcgccag cagttcgtca cggtttttcg gttcaccgtg
    13081 gaaaagttgg ctgagtaaca gggagaaaaa tcccttcttg ttgcttatcg tgtcactact
    13141 gtgtgaattg tcgtcgctca tggcgtcgta tgggttctca tgttagttaa tcaaaacgcc
    13201 gtcgttaatc accaacggcg gggacgtctg ccagtcaaat gcctggcaaa ttattctttc
    13261 tcggcaatgt acggatcctc atagcccaga gcaagcataa tctctgtttc gagggcttcc
    13321 atttcttctg cttcgtcatc ttcgatgtga tcgtaaccta acaaatgcag actgccgtgc
    13381 accaccatat gcgcccagtg cgcctccagt ggtttgcctt gctcctgagc ttccttctca
    13441 accacctgac ggcagataac cagatcgccc agtagcgaca tttccatgcc aggcggcact
    13501 tcaaacggga aggagagcac gttggtcggc ttatccttac cgcgataggt cagattcaga
    13561 ctgtggcttt cggcggtatc gaccacgcga atagtcactt ccgattcttc ctgaaactgc
    13621 gggatcaccg cattcagcca tgtttgaaac tggctctctt ccggtaaccc ggaattatct
    13681 tcacatgcca gttgtaaatc gaggatcacc tgactcattt ttgttcctgt tcttcgcgct
    13741 tgcgttctgc cgccagcgcc gcttttcgtt tttgttcggc ttcttcccag gcttcatagg
    13801 cgttaacgat acgcgccacc acagggtgac gaaccacgtc ttcgctgtgg aagaagttaa
    13861 agctgatctc ttcgacatcg gccagcactt cgatggcgtg acgtaagcct gatttagtat
    13921 tacgcggcag gtcgatctgt gtgacgtcgc cggtgataac cgcttttgag ttaaaaccga
    13981 tacgggtcag gaacatcttc atctgttcga tggtggtgtt ctggctctca tcgagaatga
    14041 taaacgcgtc gttcagcgta cgaccacgca tataggccag cggcgcgact tcaataacgt
    14101 tgcgctcaat cagtttctca actttctcaa agcccagcat ttcaaacagc gcgtcgtaca
    14161 gcgggcgcag atacgggtct actttctggc ttaaatcgcc aggcaggaag cccagtttct
    14221 caccggcttc gaccgccgga cgagtcagca gaatacggcg aatctcctga cgctccaggg
    14281 catcaactgc cgcagccact gccaggtagg ttttacccgt acccgccggg ccaacgccga
    14341 aggtaatgtc atggtcgaga atattggcga tgtactgcgc ctggtttggc gtgcgcggct
    14401 taattacgcc gcgtttggtt ttgatattga ccgctttgcc gtactccggc acgctctccg
    14461 cactttgctc cagtactcgc gcttctttaa tcgcaaggtg gatctgttcc ggttcgatat
    14521 cctgaatctg accgcgcatc ggggctgtat cgacatacag gctacgcaga atgtctgccg
    14581 cagcggtgac gcaaatcgga cggccggtca gtttaaagtg gttgtcgcgg cgattgatct
    14641 cgatgccgag acggcgttcg agctgcttga tgttgtcatc aaacgggccg cacaggctca
    14701 acagacgcgc gttgtctgct ggctccaggg tgatttcgcg agtgtctatg ttcaaaccgt
    14761 tcctcttatc tgtatgtcgc cggaagctga acattcaccg gcctataagg aaattattca
    14821 cgccacagga aaaaggcgca agcattgcga taaagatggg gataaagaga gaaaaaacaa
    14881 ggcccaccgg aacggcgggc ctgagaatta cggctgataa taacccacgc caaggtcgtt
    14941 ttctttgcgg gtacgggcaa tcaccgattc cggtgtttct gccacgcgca gacccatttc
    15001 atcttcagta cgcaccactt taccgcgcag agagttcggg tagacgtcgg taatttctac
    15061 atcgacgaat ttaccgatca tatccggtgt gccctcgaag ttgaccacgc ggttattttc
    15121 ggtacgcccg gaaagctcca tgatgctctt acgcgaagtc ccttctacca gaatacgctg
    15181 ggtggtgccg agcatccggc ggctccatgc catcgcttgc tgattgatac gttcttgcag
    15241 aatatacaga cgttgcttct tctcttcttc cggaacatca tcaaccatat cggcggctgg
    15301 tgtacccgga cgtgcagaga agataaagct gtagctcatg tcgaaattga cgtcggcaat
    15361 cagcttcatc gttttctcga agtcttcggt ggtttcgcca gggaagccaa cgatgaaatc
    15421 agaactgatc tgaatatctg gacgcgccgc acgcagttta cggatgatcg ctttgtactc
    15481 cagcgccgta tgggtacgtc ccatcaggtt cagaatgcga tcggaaccgc tctgtaccgg
    15541 cagatgcagg aagctcacca gctccggcgt gtcgcgatac acttcgatga tatcgtcggt
    15601 gaattcgatc ggatggctgg tggtaaagcg aatacgatcg atcccgtcga tcgcagcaac
    15661 cagacgcaac agatcggcaa acgatccggt ggtgccgtcg tagttctcac cacgccaggc
    15721 attcacattc tgaccgagca ggttgacttc acgcacgccc tgagccgcaa gctgggcgat
    15781 ctcaaacaga atatcgtcgg acggacggct tacctcttca ccacgggtgt aaggcaccac
    15841 gcagtaggtg caatatttat tgcagccttc catgatggag acaaacgcgg tcggcccttc
    15901 ggcgcgcggt tccggcagac ggtcaaactt ctcgatttcc gggaagctga tatctacaac
    15961 cgggctgcgg tcgccgcgca cggagttgat catctccggc agacggtgca gcgtttgcgg
    16021 cccaaaaata atatcgacat agtgggcgcg ctggcgaatg tgctcgcctt cttgcgatgc
    16081 cacgcagcca ccgacgccga taatcaggtc tggattcttc tcttttaaca gtttccagcg
    16141 acccaactga tggaagactt tttcctgagc cttctcgcgg attgagcagg tgttcagcag
    16201 cagcacatcc gcttcttccg ccacgtcggt cagttgatag ccgtgggtgg catccagcag
    16261 atcggccatc ttcgatgaat cgtactcgtt catctgacag ccccaggttt taatatggag
    16321 ttttttggtc atcgacttgc tcttgcgaaa tagtagccag gaatgcaggg cgcatagtgt
    16381 aatgctttgc tgccgttgtg accagtatga gcgttatcag cccttggggg taaaaatcct
    16441 gtaaacttaa agcatattgc taacaggatg attgaccatg acaaatcaac caacggaaat
    16501 tgccattgtc ggcggaggaa tggtcggcgg cgcgctggcg ctggggctgg cacagcacgg
    16561 attttcggta acggtgatcg agcacgcaga acctgcaccg tttgtcgctg atagccaacc
    16621 ggacgtgcgg atctcggcga tcagcgccgc atcggtatca ttgcttaaag ggttaggggt
    16681 ctgggatgca gtacaggcta tgcgttgcca tccttaccgc agactggaaa cgtgggagtg
    16741 ggaaacggcg catgtggtgt ttgacgccgc tgaacttaag ctaccgttgc ttggctatat
    16801 ggtggaaaac accgtcctgc aacaggcgct gtggcaggcg ctggaagcgc atccgaaagt
    16861 aacgttacgt gtgccaggct cgctgattgc attacatcga gataatgatc ttcaggagct
    16921 ggaactgaag ggcggtgaaa cgatccgcgc gaaactggtg attggtgccg acggcgcaaa
    16981 ttcgcaggtg cggcagatgg cgggaattgg cgttcatgcc tggcagtatg cgcagtcgtg
    17041 catgttgatt agcgtacagt gtgagaacga tcccggcaac agcacctggc agcaatttac
    17101 cccggacgga ccgcgtgcgt ttctgccgtt gtttgataac tgggcatcgc tggtgtggta
    17161 tgactctccg gcgcgtattc gccagttgca gaatatgaat atggcgcaac tccaggcgga
    17221 aatcgcgaag catttcccgt cgcgtctggg ttacgttaca ccgcttgccg ctggtgcgtt
    17281 tccgctgacg cgccgacatg cgttgcagta tgtgcagccg gggcttgcgc tggtgggcga
    17341 tgccgcgcat accatccatc cgctggcggg gcagggggtg aatcttggtt atcgtgatgt
    17401 cgatgccctg attgatgttc tggtgaatgc ccgcaactac ggtgaagcgt gggccagtta
    17461 tcctgtcctc aagcgttacc agatgcggcg catggcggat aacttcatta tgcaaagtgg
    17521 tatggatctg ttttatgccg gattcagtaa taatctgccg ccgctgagtt ttgtgcgtaa
    17581 tctcgggtta atggcggcag agcgtgctgg cgtgttgaaa cgtcaggcgc tgaaatatgc
    17641 gttagggttg tagccttaca acattgccgg atgcgtgcca accgtaggtc ggataagacg
    17701 cggcagcgtc gcatccgaca ttgaaggata agacgtgtca acatcgcatt cgacattgaa
    17761 tgaacgcaga aaagcaaaaa gctcgccgaa gcgagctttt ttaatgtggc tggggtacga
    17821 ggattcgaac ctcggaatgc cggaat
//
LOCUS       DAZKIV010000097        17478 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZKIV010000097 DAZKIV010000000
VERSION     DAZKIV010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17478
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.097"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(109..840)
                     /gene="rlmB"
                     /locus_tag="ACHOT6_003125"
     CDS             complement(109..840)
                     /gene="rlmB"
                     /locus_tag="ACHOT6_003125"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFP9948300.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1020..3461)
                     /gene="rnr"
                     /locus_tag="ACHOT6_003126"
     CDS             complement(1020..3461)
                     /gene="rnr"
                     /locus_tag="ACHOT6_003126"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFP9948301.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3500..3925)
                     /gene="nsrR"
                     /locus_tag="ACHOT6_003127"
     CDS             complement(3500..3925)
                     /gene="nsrR"
                     /locus_tag="ACHOT6_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFP9948302.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4130..5428)
                     /gene="purA"
                     /locus_tag="ACHOT6_003128"
     CDS             complement(4130..5428)
                     /gene="purA"
                     /locus_tag="ACHOT6_003128"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFP9948303.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVRDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5532..5729)
                     /gene="yjeT"
                     /locus_tag="ACHOT6_003129"
     CDS             complement(5532..5729)
                     /gene="yjeT"
                     /locus_tag="ACHOT6_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFP9948304.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5811..6815)
                     /gene="hflC"
                     /locus_tag="ACHOT6_003130"
     CDS             complement(5811..6815)
                     /gene="hflC"
                     /locus_tag="ACHOT6_003130"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFP9948305.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6818..8077)
                     /gene="hflK"
                     /locus_tag="ACHOT6_003131"
     CDS             complement(6818..8077)
                     /gene="hflK"
                     /locus_tag="ACHOT6_003131"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFP9948306.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8163..9443)
                     /gene="hflX"
                     /locus_tag="ACHOT6_003132"
     CDS             complement(8163..9443)
                     /gene="hflX"
                     /locus_tag="ACHOT6_003132"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFP9948307.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALMERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9519..9827)
                     /gene="hfq"
                     /locus_tag="ACHOT6_003133"
     CDS             complement(9519..9827)
                     /gene="hfq"
                     /locus_tag="ACHOT6_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFP9948308.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9913..10863)
                     /gene="miaA"
                     /locus_tag="ACHOT6_003134"
     CDS             complement(9913..10863)
                     /gene="miaA"
                     /locus_tag="ACHOT6_003134"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFP9948309.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10856..12703)
                     /gene="mutL"
                     /locus_tag="ACHOT6_003135"
     CDS             complement(10856..12703)
                     /gene="mutL"
                     /locus_tag="ACHOT6_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFP9948310.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKRPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12713..14050)
                     /gene="amiB"
                     /locus_tag="ACHOT6_003136"
     CDS             complement(12713..14050)
                     /gene="amiB"
                     /locus_tag="ACHOT6_003136"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFP9948311.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14069..14530)
                     /gene="tsaE"
                     /locus_tag="ACHOT6_003137"
     CDS             complement(14069..14530)
                     /gene="tsaE"
                     /locus_tag="ACHOT6_003137"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFP9948312.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14502..16049)
                     /gene="nnr"
                     /locus_tag="ACHOT6_003138"
     CDS             complement(14502..16049)
                     /gene="nnr"
                     /locus_tag="ACHOT6_003138"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFP9948313.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16048..17187
                     /gene="queG"
                     /locus_tag="ACHOT6_003139"
     CDS             16048..17187
                     /gene="queG"
                     /locus_tag="ACHOT6_003139"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFP9948314.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17170..17223)
                     /locus_tag="ACHOT6_003140"
     CDS             complement(17170..17223)
                     /locus_tag="ACHOT6_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948315.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3982 a         4969 c         4375 g         4152 t
ORIGIN      
        1 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
       61 gacaaaaaaa ccatccaaat ctggatggct tttcataatt cagacgaact agctgcgctg
      121 acgaaccgct tcaaataagc aaattccggt tgcaaccgaa acgttcaggg aagaaacact
      181 tcctgccatt gggatgctga tcaactcatc gcaatgttca cgggtcaggc gacgcatacc
      241 ttcaccttcc gcgcccatca ccagcgccag gcgtccggtc attttgctct gatagagtgt
      301 atgatccgcc tcgcctgccg taccgacgat ccagatattc tcttcctgca acatacgcat
      361 ggtgcgcgca aggttagtca cccgaatcag tggaacgctt tctgccgcgc cgcaggctac
      421 ttttttcgcc gtagcgttga gctgtgcgga gcgatctttc ggcacaatca ccgcgtgaac
      481 gccagcagcg tccgcgctac gcaggcacgc gccgaggttg tgcggatcgg ttacaccgtc
      541 gaggatcagc aggaacggtt gatcgagcga agcgatcaga tccggcagat cgttttcctg
      601 atactgacgt cctggcttca cgcgggcgat aatgccctga tgcacggcac cgtcgctttt
      661 ctcgtcgaga tattggcggt ttgccaactg gataaccacg ccctgagact caagggcgtg
      721 aatcagcggt aacagacgtt tatcttcacg gccttttaaa ataaagactt cctgaaaacg
      781 ttcaggggcg cgctccagca gggcctgcac tgcgtggatg ccgtaaatca tttcgctcat
      841 taatgtactc gttgtttacg ttttgcctga tgcactacgt ttatccggcc tacatgatct
      901 ctgcaatata ttgaatttgc gtgattatgt aggccggata aggcgttcac gccgcatccg
      961 gcatgaacaa agcgcatttt gtcagcaatc taaccctctt cttttaaaga gggtattgat
     1021 cactctgcca cttttttctt cgccgcacgc ttcgctttgg tcgctgcggc tattttctgc
     1081 gttttcgccg atggcttttt cgcttttctc gcgtctttct tcgccgcttt cggcttcgtt
     1141 tttttctcac cgcggaaggc gctgtctggc tcaaagttta cctttttacc gacctgacga
     1201 cgcttgccgc cttttttgcc tgcatcgcct tttttcgctt tctcgcgcgc cgttttaccg
     1261 acgttgcgcg gtgcgcgttc actggagatc agactaaagt cgattttgcg ctcgtccata
     1321 ttaaccgctt cgacgcgaac ttccacgcga tcgcccaggc gataagtctg gccgctggat
     1381 tcccccatca ggcgttgccc tacctggtca aagcgatagt agtcattgtc cagcgaagag
     1441 acatggacca gaccatcaat gaacaagtcg tccagacgga cgaagaagcc aaagccagtg
     1501 acgctggaaa ttacgccttt aaagacgtta cctacctggt cgagcatgaa gtcacacttc
     1561 agccagtcag cgacatcgcg cgttgcttcg tcggcacgac gttccgccat cgaacagtgc
     1621 tgacccagtt gcaacatctc ttccatcgaa taatggtagc cgccggtttc agtggtgttg
     1681 ccctgatgcc cctgctcttt cgccagcaga tatttaatgg cgcggtgcag cgtcaggtca
     1741 ggataacgac gaatcggcga agtaaagtgc gcataggact gcaatgccag accgaagtga
     1801 ccacggtttt ctggatcgta aatcgcctgt ttcatcgagc gtagcagcat ggtttgcagc
     1861 atttctgcat caggacggtc agcaaccgac tccagcagtt ccgcgtagtc acgcggttcc
     1921 ggcttattac cacccggcag ctccagcccc agctccgcca gcactgaacg gaaagaggta
     1981 atcgcttcgg tgctcggctt gtcgtgaata cggaacagtg ccggttcttt cgctttctca
     2041 acgaaacgcg ccgccgagat attcgccaga atcatgcact cttcaattaa tttgtgcgcg
     2101 tcgttacgct gggtctgttc gatacgttca atacggcgtt cagcgttgaa aatgaacttc
     2161 gcttcttcgc tctcaaatga gatcccaccg cgttcttcac gggctttatc cagcacttta
     2221 tagaggttat gcaactcttc gagatgctta accagcgggg cgtactgctc gcgcagatcc
     2281 tgatcgccct gcagaatatg ccagactttg gtgtaggtca gacgcgcgtg agagctcatc
     2341 accgcttcgt agaatttgta gcccgtcagg cggcctttcg acgaaaccgt catctcgcac
     2401 accatacaca ggcggtctac ctgcgggttg agcgagcaca ggccgttaga gagcacttcc
     2461 ggcagcatcg ggataacctg cgaagggaag tacaccgacg taccacgatt acgcgcttct
     2521 ctgtccagcg gcgttggcgg acgcacatag tagctgacgt cggcaatcgc gacccataaa
     2581 cgccagccgc cgccgcgttt tttctcgcag taaactgcat cgtcaaagtc acgggcgtct
     2641 tcgccatcaa tggtgaccag cggtaaatcg cgcaaatcga cacggcccgc ttttgcttct
     2701 tccggcactt cttctttcag cccggcaacc tgttgctcaa cagcctgcgg ccagatgtac
     2761 ggaatttcat gggtacgcag agcgatatca accgccatgc cggtgcccat attatcgcca
     2821 aggacttcga cgattttacc caccgctttg gtacggcgag tcgggcgttg ggtcagttca
     2881 acaaccacca caaagcccat ccgcgcgccc atgatctgat cgggcgggat taagatatcg
     2941 aagctcagac ggctgtcgtc aggaaccaca aagccgacgc ccgcttcggt aaagtagcga
     3001 ccaacaatct ggctggtttt tggcaccagt acgcggacaa tacgcgcttc acgacgacct
     3061 ttacggtcag cgcccagcgg ctgagccagc acctgatcgc catgaatgca ggttttcatc
     3121 tgctcgctgg agagatacaa atcatcttta cgcccttcaa cccgcagaaa gccgtagcca
     3181 tcacggtggc caataacggt acctttcacc aggtcgaggc gttccggcag cgcatagcac
     3241 tgacgacgag tgaagaccag ttgaccatcg cgctccatcg cgcgcaggcg gcgacgcagg
     3301 ccttcaagct gctcttcgcc ttcaatgtgc agttctaccg ccagctcatc acggctggcc
     3361 ggtttttcac gtttagttaa atgttcgagg ataaattccc gactagggat gggattcgcg
     3421 tatttttcag cttcgcgttc ctggaaagga tcttgtgaca tctcggttcc tccgttgtca
     3481 tctctgatga agattttcgt cactccacca gcaataattt ataaagcggt tgattctctt
     3541 caaccaaatc ggcaagcgtg tagttatcca gttccgtaag aaaactttgc acggccttag
     3601 aaagcgcctg tttcaaccga caggcaggtg taatgtggca aaactcactg ctgcaattca
     3661 ccagcgataa gggctccagc tcgcgcacca catcaccaat acgtatcgca ctcgccggtt
     3721 tacccaggcg aatgccgcca ttttttccac gaacagcagt cacgtagccg gcacgactaa
     3781 gttgattgat tattttgacc atatgattac gggagacgcc gtagacgtca gtcacttcag
     3841 aaatactggt catccgccct tctggcaatg acgccatgta gatcagcgca cgtaatccgt
     3901 aatcagtgaa actcgttaac tgcacatcaa cctcaaaagg gaaatcggga aaaaatacat
     3961 ttatattgat gataaaccag ccacaagctg tgtcgctaat ttatttcagt tcggggaagg
     4021 aaaaaagcga ggatttaaca ctgtgtcgga tagcgtaatg gcttaaccga cacagcaatg
     4081 tatcacactg ttagcccggc aggcaaaata tctgccaggc gtaccagaat tacgcgtcga
     4141 acggatcgcg cagaatcatg gtttcagtac gatccggacc ggtagagatg atatcgatcg
     4201 gcacaccagt cagctcttca atacgcttga tgtagttcag cgccgcctgc ggcagaccgc
     4261 tacgatcttt cacgccgaag gtggattcag accagcccgg catggtttcg taaatcggct
     4321 ctacaccttt ccagtcgtca gctgccagcg gagtggtagt cacttcgcga ccatccggca
     4381 tacggtaagc cacgcagagt ttaacctctt tcaggccatc cagaacgtcc agtttagtca
     4441 ggcagaagcc agacagggag ttcagctgta ccgcacgacg aacggcaacg gtgtccagcc
     4501 agccggtacg acgacgacga cccgtagttg cgccgaattc gttaccctgc ttgcagagga
     4561 actcgccagt ttcatcaaac agttcggtcg ggaaaggacc tgcacctaca cgagttgagt
     4621 aagctttgag gatacccaga acgtaatcaa cataacgcgg gcccaggccg gaaccggtcg
     4681 ccacgccacc agcagtggtg ttggaagaag ttacgtacgg ataagtaccg tggtcgatat
     4741 ccagcagcgt accctgcgca ccttcaaaca tgacgaaatc gccacgctga cgcgcctggt
     4801 cgagcaggtc agaaacgtca accaccatag aagtcaggat gtcggcaaca gccatcgtat
     4861 catcccgaac tttctggtaa tcaaccgctt cagctttgta gtagttaacc aactggaagt
     4921 tgtgatattc catcacttct ttcagttttt cagcgaaggt ttctttgtcg aaaaggtcgc
     4981 caacacgcag accgcgacgt gctactttat cttcataagc aggcccgata ccacgaccgg
     5041 tggtgccgat cgctttcgcg ccacgcgctt tctcacgcgc gttatccagc gcaacgtgat
     5101 aatcaagtat cagcggacat gcttcagaca gcagcagacg ctcacgaacg gggatgccac
     5161 ggtcttccag ttctttcatc tctttcatca gcgcggccgg agacagcaca acaccgttac
     5221 cgatgatgct ggttacattc tcgcggagaa tacctgatgg aataagatgg agaacggttt
     5281 tttcaccgtt gattacgaga gtatggcctg cgttgtgacc gccctggtag cgtacaacat
     5341 atttagcccg ttcagtcaga agatcgacga tcttaccttt accttcgtca ccccattggg
     5401 tgcccagtac gacgacgttg ttacccattt ttcaaaatca ccgtttgctt aaaaatggat
     5461 tctaccatcg ctttttcaga gttacagcac tttttgcact caaaaatgac ctcgatcagt
     5521 ctgttttttg ttcagccaat cgttttcctc aacatgtagt agaccacaac gcccgcaacc
     5581 acaagtccac cgccaaaacg acgtaaaata ttatcgggca aattggtcat cgcagagatc
     5641 atcttcttcc atgccttcgg gtaaagcatc ggccctaaac cttccagtac caaaaccagg
     5701 gcaagcgcca gccagattgt cgaattcatt cattatcctt atagaaaaag aaaaccaccg
     5761 acatccctga ggatgcggtg gctttattga cctgtaccgc agtcgttata ttaacgcgtt
     5821 gcggaagtcg gcgtcttcat gtagcggaag aaatcgctat ccgggctcat gaccatcacg
     5881 tcctgattgc cagagaagct gttctcataa gcacgcaggc tacggatgaa tgcgtagaag
     5941 tccggatctt tactgaatgc atcagcaaac agtttggctg cttcggcatc gccttcacca
     6001 cgcatgatgc ggccctgacg ctcagcttct gccagcgttc tggtcacttc atagtcggca
     6061 gtcgcgcgca gtttttccgc ttcttcctga ccttgtgaac ggtgacgacg cgctaccgct
     6121 tcacgctcgg cgcgcatacg gttgtagatc gcttcagaca cttcggtcgg caggttgatc
     6181 tgcttgatac gcacatcgac aacttcaata cccagcgccg ccatactgtt cgggttgatg
     6241 accggaactt tgcccttcgt ctcagccgtt acgcgctctg ccgcttcggc aatggcgtta
     6301 tctgctgccg gggtagtaac ttcatcttct gtacccgcag aaccggagtt cagcgcgtca
     6361 cgtacttcga gggtcagacg accacgggaa tcagtgacga tatctttcac gtccaggcga
     6421 ccaatttcag aacgcagacg gtcagagaac ttacgtttca acagcacttc cgcttgcgaa
     6481 atgtcgccac cacccgttgc caggtagtaa cggctgaaat cgctgatgcg ccatttgatg
     6541 taagaatcga cgatcaggtc tttcttctct ttggtcacaa agcggtcggc ctggttgtcc
     6601 atggtctgaa tacgtgcgtc gagcattttc accgtttcaa tgaacggtat cttgaaatgc
     6661 agacccggct cataaaccag aggtttgttg tcatcgtcac gcagtacctt accaaaacgc
     6721 agcgtaatac cgcgctcacc ttctttgacg acaaagacag acatgtaaag cactaccagc
     6781 acgatgatga taatcgcgat aactgactta cgcatcgtta ttccccctga cgctggtagt
     6841 cgttacgctg cgcgttggcg cggcgttggt ccataatatc gccctgactg gtggacgacg
     6901 tgttgcttgc tccactggtt gtggaggaag aggctggcgg cagacgcagc agattgctgg
     6961 caccgttatc gctcttcgcc gcaggggcgt taccaccttt cagcatctgg tctaacggca
     7021 gaaccatcag gttgccacct ttgtcgttaa ccagcacttt gcgggtgttg cccaacactt
     7081 tttccatcgt ctcgatatac agacgctcgc gagtaatttc cggcgcggct ttatattccg
     7141 gcagaagttt agcaaagcgc gccacttcac cctgagcttc caggatggtc tgggccttgt
     7201 acgcacgcgc ctcttcgagg atacgttgcg cctgaccgtt cgcacgcggc tgaacttcgt
     7261 tggtatacgc ttctgcttca cgaatgtatt gctgttcgtt ttcacgcgcg gcaatcgcat
     7321 cgtcaaacgc cgcttttact tcttccggcg gacgagcagc ctggaagttg acgtccagca
     7381 gcgtgatacc catgtcatac ggacgaatcg tctcttccag ttcgcgctga gtatcgctac
     7441 gaatcacggt acgaccttcc gtcagaatgc ggtccatggt gtatttaccg ataactccac
     7501 gcagggcgct gtcggtagcc tgacgcaggc tgtcatccgg gctggtcacg ctatacagat
     7561 atttttccgg attggtgacg cggtactgca cgttcatctc aacgcgcact acgttctcgt
     7621 ccgacgtcag catcacacca gaagcggcca gttcacgcac ggcttccacg ttcaccggtt
     7681 tgacttcgtc gataaacgtc ggtttccagt tcagacccgg ctcaaccaga tggctgaatt
     7741 taccaaagcg tgttaccacg ccgcgttcgg cttctttaat ggtatagaaa ccactggccg
     7801 cccagataat gacaatcgct gccgctgcga tggtaacgac acgaccgcca agctgcgggc
     7861 gcgggccttg cgatgaactg ccaccgccag atccggtgcc tttaccgccg cccagaccac
     7921 cgagcttttt gctcagtttg cggaagatat catctaaatc aggtggccct tgatcgcgac
     7981 cgcctttgtt tccatttccc tcagagttgc cgccaggttt gctgcttccc cacgggtcgc
     8041 ggtcttgtcc gttattaccg ggctgattcc acgccatgtt tgtgctccat atttgttatg
     8101 cggtgatccc tgttgtcttt cgacgccaga ggatatgact ccacgcttca gacgctacgc
     8161 cgttagatca ggtaatcgat caacgccggt tcttgtttac agaggcgacg ccagtcaacg
     8221 atcggcatac gaacttgcag acttacgctg ccgtcctcct ccatccactc tttttctatt
     8281 gcctgaagct gataaaaacg acttctcaga cgtccttcct gcggcggcaa acgcaatgta
     8341 tgctgcgcca cctcgccgga aagccgctcc atcaaagcct gaaaaagctg tggtatcccc
     8401 gctccggtct gtgcggaaag ccagacacgg atcggtttgt tctcttcgtc ccgatcaata
     8461 cgcggttcga aatcttccag catatcgatc ttgttcatca ccagcagggt tgggatctcg
     8521 tgagcgtcga tctcttcaag aaccgtattc accgcttcga tgttttcttg tacacgcaca
     8581 tccgccgcat caatgacgtg cagcagtaat gttgcttgcc gcgtctcttg taacgtggct
     8641 ttaaatgccg ccaccagatc gtgcggcagg tggcgaataa accctacggt atctgcaagt
     8701 acggtttcac cgacatctgc aacgtcaata cgccgcaacg tcgggtcgag ggtggcaaac
     8761 aactggtctg ccgcgtagac ccgcgcttcg gtgatgcgat tgaaaagggt agatttaccg
     8821 gcgttggtat atcccaccag cgaaacagta ggaacgtcgg ctttgatacg cgattgccgc
     8881 ccctgctcac gctgcttttc aactctttcc aggcgcgact gtatctgcac gatgcgatta
     8941 cgcaacaaac gacggtcggt ttcgagctgg gtttcacccg gaccacgcaa acctatcccg
     9001 cctttctgtc tttcaaggtg ggtccagcca cgcaccaggc gcgtagccag atggcgcagc
     9061 tgcgccagct caacctgcaa cttaccctca tgggtacgcg cacgttgggc gaaaatatct
     9121 aaaataaggc cggtgcggtc gataacacga cactcgcaca aacgctccag gttacgctct
     9181 tgcgccgggc tcagggcatg gtcaaaaaga acgaccgaag cacccgtcgc tttgacagct
     9241 tccgcaattt caactgcttt accttcacct acaaaatact ttgggtgcgg cgctttacgg
     9301 ctaccggtaa tcacctgcaa tgcttcgaca ccggcggaag agaccagaga ttcaaactcc
     9361 tggaggtctt ccatatcttt gtcttgcgta aaatagatgt gtaccagtac cgcctgctca
     9421 ccagcatcat aacggtcaaa caagcgtata accctctaaa tagatcagcg gggaacgcag
     9481 gatcgctggc tccccgtgta aaaaaacagc ccgaaacctt attcggtttc ttcgctgtcc
     9541 tgttgcgcgg aagtattctg cgcgctgcta ccatgatggt agttactgct ggtaccgcca
     9601 ccggcgttgt tactgtgatg agaaaccgga cgagacggga caacagtaga aatcgcgtgc
     9661 ttgtaaacca tctggctgac cgtgtttttc aacaggatca cgaactgatc aaaagactcg
     9721 atttgccctt gcagcttaat accattcacc aaataaatag aaactggaac acgttcccga
     9781 cgcagtgcgt tcaggaacgg atcttgtaaa gattgcccct tagccattct ctcttttcct
     9841 tatatgctta tttgtacttt gaacctttcg attctgaaaa aattgcgcac gatacgtctc
     9901 aattgtacac attcagcctg cgatagcacc aacaacctgt aatacttcgt cacgcgcctg
     9961 ttctggtttt tcactgtcaa gccagtgaac cccttcccaa ccacgcagcc aggttatctg
    10021 ccgcttcgcc aactgtctcg tggcgcaaac acctcgataa accatttcat cgtatgagat
    10081 ttcgccttca aggtaagacc acatctggcg ataacccacg caacgaatgg aaggcaagtc
    10141 cgtatgcaaa tctcctcggg caaaaagcgc ccggacttct gcttcaaaac ctgaagccaa
    10201 catctgatga aaacgctgct caatgcgttg atggagcagt tcacggctcg ccggggcgat
    10261 ggcgaactga tgcacctgat acggtagagc gtctcctgac gtttgcgtca gttccgttaa
    10321 agttttaccc gaaatgaaaa aaacttccag tgcccgggaa agcctttgtg gatcatttgg
    10381 atgaatcctt gctgccgcaa ccggatctac ctcctgaagt tgacgatgca atgactccca
    10441 accttgctct gccgcctgtt gctcaattct ggcccgcact tccgggtctg ccgacggtag
    10501 cggcgacaac ccttccagca atgccttgaa atacaacatc gtaccgccca ctaacagtgg
    10561 gatccgcccc gccgcggtga tatcggccat ttccgccagc gcatcgcggc gaaaatcagc
    10621 agccgagtaa gcctgcgacg gatcgcgaat atccagcaat cggtgcggcg cggcgagtaa
    10681 ctcttcagcg ttcggcttcg ccgtcccgat atccatccct ttgtaaataa gggcagaatc
    10741 aacgcttatc aactctactg gtaaaatttt acgcagctca atggctaacg ccgttttacc
    10801 ggaggccgtc ggccccatca aaaaaatcgc cttaggcagg ctcgccttac tgatatcact
    10861 catctttcag ggcttttatc gccggatgta aatcaacaga ttgtaacaga ccacccggcg
    10921 gccttttcac aagttgcgga cataaccgtt ccacgtccgc cagcagggtt atggcctgtg
    10981 ccattgacca ctgcgcatgt tcgctcatca gatttcgtgc aatccactgc gcaatattgc
    11041 caggttcgaa tacggactgc ttcgccaggt agcctatcag ttcaggaatc aagatttgta
    11101 aattttgttg gcgtaagggt aaaggcacgg ccctgatggt cacatgctgt gcatctgact
    11161 ggaaatcaat acccaattcc gccagggcag actgcgcttt ttctaatgcc gatttttctt
    11221 cggcagaaac ttttagccgc aacggaatca gcagcggctg ggcgcaaacg ggcgcttcac
    11281 ccggcgtcaa ttgtgcctga cgtagccaac gttctgccac tggcaaggat aaaagtgaaa
    11341 tgttgccatc gcgctccagc aacgcacagt cggaatggac gatagtcagt acccgaccaa
    11401 aactctgact gttcgccgca agtgcaggtt cctgcggttc cggcgctttt ggtttttgca
    11461 tcggcgcggg cgtttgcaaa agctggcgat acacttcacc ttgctgtttc tggtagcctg
    11521 gctgcgcatt cggccagggg gcagccggac gactgcctga tgctggcgca ggagtgtagc
    11581 gcggagctac cggctcacga gctgccggtt ctgcaaagtg attgcgcccc gccgccacgc
    11641 ggttttccgg aatggaacgc ggtgcaggtt ggggttcatc gtccagcggt agcggcgttt
    11701 ccagttgctg ttgtagcacg ctcagcacgc cctgatagat aaaatcatgc accagacgcg
    11761 actgatggaa acgcacttcg tgtttggcgg ggtgcacgtt gacgtccacc tgatgcggat
    11821 cgatctccag atacagcaca aatgccggtt gctgatcggc ccccagtttg tcttcgcagg
    11881 cctggcggat cgcgtgattg atcaggcgat cgcgcatcat gcgaccgttc acgtagcaat
    11941 actgaatttc tgccagtgcg ggcgtggtgt gatttggatc ggccacccag ccgcgtagcg
    12001 tgagatcgcc gtgttgccat tcaatcgcca gcgcttgttc aagaaaagcg gtgccgcaaa
    12061 tcgcgcctaa gcgccgttct ttttgcccgc cttccggcac tgcgcggtac tgacgcacaa
    12121 ttttaccgtt atgcgacagg ttgatcgtga cgtcgaaacg cgccagcgca atgcggcgga
    12181 tgatctcatc aatgtggtta aattcggttt tctcggtgcg caggaatttg cgccgcgccg
    12241 gggtgttgta gaacagatcc agcacctcca gcgtcgtccc caccggatgc gccgccggtt
    12301 ttaccgtcac gttcatatcg cgcccttcgg cataggcctg ccaggcttcc tgctgttctg
    12361 cggtgcgtga agtgagcgtc aggcgggaaa ccgaactgat actcgccagc gcctcaccgc
    12421 gaaagcccag gctgataatg gcttcgagat cgtccagaga ggcgatttta ctggtggcat
    12481 gacgagccag cgccagcgcc agctcatctt ttttgatacc gcagccgtta tcacgaatgc
    12541 ggataagttt cgccccaccg cgttcgatat caatatcgat acgcgtcgca cctgcatcga
    12601 ggctgttttc caccagttct ttgactaccg acgcaggtcg ctcgaccacc tcacctgcgg
    12661 caatctggtt cgccagttgt ggcggtaaga cctgaattgg catcaatcgt ccttagtttg
    12721 gcagcgtgcg atctggcgtc gtcaccgtac tggcagtttg tgccgttgcc ccctgcggcg
    12781 cagattgcat cggatgcgca aggaaataat tgcgcaggcc tttgtaaatg gcttctgcca
    12841 gctgttgttg gtaatcgtcg ctcgccagca aacgttcttc gctgttgttg ctgataaaac
    12901 cggtttcgac cagtactgac gggatatccg gcgaacgcag aacgccgaga ctcgcgtgtt
    12961 ctggtcggcg tttatgtatt tcgccaatgc gttgcaactg actgatcata ctggtcgcta
    13021 catcataccc tacccgttgg gaatgaccga actgtaaatc cagcaccgcc tggcttaaat
    13081 aggggtcaga ctgactgttc gccagcacat cacccgcccc gcccagcagc tccgactgtt
    13141 tctcatgctg ttccagccag ctggccattt cgctgttggc gcgacggtta gagagcaccc
    13201 atacggaagc gccagtcgca ctgcggttcg gtgcggcatc agcgtgaatc gacacgagga
    13261 aattggcgtt ttgcttacgt gccacatcac tgcgccccat caccgagata aagtaatccc
    13321 cgtcacgggt taaaacgcct ttaaacatcg gatcgtcatt gagcaaagta cgcaatttac
    13381 gcgcgatggc gatggtgaca tttttctccc gcgtaccacc ggggccgata gcgccagggt
    13441 cctgaccgcc gtgtccggca tcaatagcga tgataatttt atcgccagtg ttagccgtcg
    13501 cgcgcgctgc cggacgcgtt accgtattac tgctgataac acccgtagtg cggttacttt
    13561 ccgttttaaa cggattgcgc gccggttcgc tgacgcgcgg tgcgacaacc gcaggcgttt
    13621 caacgcgttt cgcaaccacg ggcggcggag gaggcggtgg cggcacatcg gcgttaatcg
    13681 taaagacgac agtgtaattg ctgccattct gccgcttcac cgcttcggtt ttaccgtttt
    13741 cggtaagatc gaccaccagc cgtagcgttt gtgcatcttt aggcgttcca gagcgaatcg
    13801 ccttcaccag attattgccg ctgaacaaca acggcagtcc ctgaatcacg cccgtttgtt
    13861 tgatatcgag cgccacggtg cgtttgcttt gatggctaaa cgcataatca ggatcgccaa
    13921 taaaactcaa cgttatccgc gcctgttgat taccgttaga aacctgaata tcagagagcg
    13981 tcgcggcacc caccggcgtg cacagcagca gcagcgtcgc taccaaccaa tttctgatgc
    14041 gatacatcat cccgccacct ttcaaaggtt aaccggctaa acgcgccagc aacaattcac
    14101 ccgcagagga aaccgcactc acgcgcgcct cacggccttg tgcctgataa tcaatgtgta
    14161 tttcgacatc cgggtcagga agaacacctg taccttgttg tggccactcc accaggcaga
    14221 tggcatcgtt ggcaaaataa tcgcggatcc ccataaactc cagctcctcg ggatcggcaa
    14281 ggcggtacaa atcaaagtga taaaccatta aattgtcgag cgtatagggt tcgaccagcg
    14341 tataagtggg gcttttgaca ttaccctgat gacccagagc ttgtaaaaag ccccggctaa
    14401 aggtggtttt acctgcgcct aaatcgccat acagatagat tacggttgcg ccatcgcagg
    14461 ctttcgctac ccgctcgccc aggtctaatg ttgcctgctc atcagggagc ggaattactc
    14521 gattcatcat ggtttttatc agtcacttcc gggttaacaa tacgctgtag cgtggaaaag
    14581 agatcggttg ccagcatccc gcgcgttcca aaacgcgccg ccagtacgtc agctgccgca
    14641 ccgtgcgcga cacagcctgc acaggctgca tcatacggcg acagtttttg cccaagcaat
    14701 gcgccaataa taccagagag cacatcgccc atgccgccgc tcgccatgcc tgcatttccg
    14761 gcatcaataa tgcctaaagc gtcaggatgg gcggcgacca cggttccggc acctttcagc
    14821 accgctacgc cgccataacg ttgtaccaga cgtttggcgc aatgtaagcg gtcactttca
    14881 atttcagcga cggaacagcc taacaaccgt gcggcctcgc caggatgcgg cgtgatcacg
    14941 cgattgtgac gcttatcggg attgattgcc agcaggttca atgcatcggc atcccacaac
    15001 atcggtttgc gaaaattctc aactttttgc agggcttttt tcccccactc ttgctggccc
    15061 agaccgggac caatcaccac cacatcggcc cattccaggc tttcggtaag agagtccata
    15121 gtcagttcat gcaccatcaa ttctggtcgt gcagtcagca gcggcgcaat gttctcactg
    15181 cgggtcagta ctcggactaa accagcacca gcacgtagcg ccgcttcccc cgtcatacga
    15241 atagcccccg ccgtgccgtg atcgccaccg ataattacca gccgcccgtg atcgccttta
    15301 tgcgaagtcg ggcgacgcgg ttttagccag tgagaaagtt gttctgctga aaaccgctga
    15361 attttcgtct cctgacctgc cagccaacta tccagcccca gtgagtcaaa atgcagttgt
    15421 ccggtaacat cccgcgcttt tccagtgagc aagcctggtt tcagcgcaat aaaagtgatg
    15481 gtgtgatcgg cgttgatcac cgcgcctggc gtagcgccag tttcagccag caggccggaa
    15541 gggatatcaa ccgccacaat cggcgcagga tgggaattag cgtggtcgat taactggcta
    15601 atggattcgc ggggcgcttg ccgcaaaccg gtaccgagca gcgcatcaac aatcagatct
    15661 accgattcgg gccagacaat attcgaagca tggatctcgc cacccgcgtt taaccatgct
    15721 tcgcgtgcca gcgcggcctc ttccggcaac ggtttgtcgc tctcctgggc caacaacgtg
    15781 acctcaatgc cgaccgcttt ggccagtcgc gcgaccacgt agccatcgcc gccgttatta
    15841 ccatgaccgc acagcaccag ccagtggcgg gcgtcaggat acgccgaacg acacacctgg
    15901 aatgcggcct cgccagcgcg aagcatcagc tcatagagtg tgagccccag cacatctgcc
    15961 gcctcgcgtt ctccgcggcg gatatcgtcg gcgtaccaga cggtgtgtgg tatacttacg
    16021 gggtttttct tcattgtatg gtccgtcatg tcagagcccc tcgatctcaa tcagttagcg
    16081 caaaaaatta aacagtgggg actggaactg ggctttcagc aggtaggtat taccgatacc
    16141 gatctcagcg agtccgagcc caaactgcaa gcatggctgg acaaacaata ccacggcgaa
    16201 atggactgga tggcacgtca cggtatgctg cgcgctcgcc ctcatgagtt attgcccggt
    16261 acgctgcgcg tgatcagcgt gcggatgaat taccttcctg ctaacgccgc atttgccagc
    16321 acgctgaaaa accccaaact cggctatgtt agccgttatg cgctgggccg tgactatcac
    16381 aaacttctgc gcaaccgact caaaaagctg ggcgagatga ttcagcaaca ttgtgtttcg
    16441 ctgaatttta gaccgtttgt cgattctgcg cctattctcg agcgcccgtt agctgaaaaa
    16501 gctgggctcg gctggacagg taagcactca cttatcctca atcgcgaggc cggttcgttc
    16561 ttctttttag gcgaattgct ggtcgatatt ccgctgcccg tggatcaacc agtcgaggaa
    16621 ggatgcggca aatgcgtggc ctgtatgacg atttgcccga ccggtgccat cgtcgagcca
    16681 tataccgtcg atgctcgccg ctgtatctct tatctcacca tcgaacttga aggggcgatc
    16741 ccggaagagt tgcgaccgtt aatgggaaac cgtatttacg gttgcgatga ctgccagctt
    16801 atctgcccgt ggaatcgcta ttcacaactc accacagaag aggatttcag cccgcgtaag
    16861 ccgctacacg caccggaact cattgagtta ttcgcctgga gcgaagagaa gtttttaaaa
    16921 gtcacggaag gatcggcgat tcgtcgtatt ggtcacctgc gttggctgcg taatatcgcc
    16981 gtagcattag gcaatgcccc ttgggatgaa acgattttga cagcgctgga aagtcgtaaa
    17041 ggtgagcacc cacttctcga tgagcacata gcgtgggcga ttgcgcagca aatagagaga
    17101 cgaaatgcgt gcatagtcga agtgcaattg ccgaaaaaac agcgtctggt tcgggtgatt
    17161 gaaaaagggt taccgcgtga cgcctgattc atccacagct tgtgaataaa aataaaaacg
    17221 cattgcaatt caagacgaat aaaatcgtca agtgatcgac tcgacaaaac ggaatcttaa
    17281 tttttaactt atctatcaat aggttaaaaa aatactattc accgtgcgaa gtttctttga
    17341 aattaagatt acagcctgag gctgtggata actctgttta caagagtttg tcagagacaa
    17401 cgaaaaccag caataacgca tcgcatcgct gtggatattt tattgagaga agaatttgga
    17461 gcgggaaacg agactcga
//
LOCUS       DAZKIV010000098        17425 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZKIV010000098 DAZKIV010000000
VERSION     DAZKIV010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.098"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            153..2813
                     /gene="stcE"
                     /locus_tag="ACHOT6_003141"
     CDS             153..2813
                     /gene="stcE"
                     /locus_tag="ACHOT6_003141"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFP9948316.1"
                     /translation="MKLKYLSCMILASLAMGAFAATAADNNSAIYFNTTQPVNDLQGG
                     LAAEVKFAQSQILSAHPKEGESQQHLTSLRKSLLLVRLVKADDKTPVQVEARDANDKI
                     LGTLTLSPPSSLPDTVYHLDGVPADGIDFTPQNGTKKIINTVAEVNKLSDASGSSIKS
                     YLANNALVEIQTANGRWIRDMYLPQGAELEGKMVRFVSYAGYNSTVFYGDRKVTLSVG
                     NTLLFKYVNGQWFRSGELENNRIAYAQHTWSAELPAHWIVPGLNLVIKQGNLSGSLND
                     INVGAPGELLLHTIDIGMLTTPRGRFDFAKDKEAHREYFQTIPVSRMIVNNYAPLHLK
                     EVMLPTGTLLTDADPGEGGWHSGTMRQSIGKELISHGIDNANYGINSTAGSGESSHPY
                     VAAQLTAHTNRGNYSNGVQVHGGSGGGGIVTLDSTLGNEFSHEVGHNFGLGHYVGGFR
                     GSVHRSADQINSTWGWDSDKKRFIPNFYPAQTNRKSCLDEQCQEPFDGHSFGFDAMAG
                     GSPFSDANRFTMYTPNSSAIIQRFFENKAVFDSLSSTGFSKWNADEHKMEPYEHTIDR
                     FEQITASVRDLSENKMAEMMAEYPIVKVHMSDGNWTRDIHAPAASAENKGRILTLDHE
                     AGYNSHLFINGGEKIISKGYKKSFVSDGQTWKESDVVNTREARKPEKFGVPVTTLVGY
                     YDPKGTLSSFIYPAMYGAYGFTYSDDSENLSSNDCQLQVDTREGQLRFKLANNRVNSS
                     VMNKFHINVPTESQPTQAILVCNNKVLDTRTLTPAPEGLTYTVNGRALPAKENEGCIV
                     SVKTAQRYCLPVGQRSGSSLPDWIAGQEVYVDSGAKAKVLLSDWDNLSYNRIGEFVGN
                     VNPADMKKVKAWNGQYLDFSRPRSMRVVNK"
     gene            2897..3772
                     /gene="gspC"
                     /locus_tag="ACHOT6_003142"
     CDS             2897..3772
                     /gene="gspC"
                     /locus_tag="ACHOT6_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9948317.1"
                     /translation="MLFFLSSRRDRNLFIKDIALKMLTPNWVLCVILLIAGYQLVSVI
                     RHFWLTPATSASDLSHVSVSETAVTDEHTEENFVFTLFGTASPPLSEGKVQKTTSSLS
                     DDLLSGGDLDVRGILYSSVTEHSVAIFAHNNRQFSLGIGEKVPGYDATISAIFSDHIV
                     INYQGKNASLPLRYDNPAKRNAQDDNNLIVGPVTTQANFRVKNIFDIMSLSPVTVNNT
                     LSGYRLSPGKASSLFYNAGLHDNDLAVLLNGSELRDTRQAKQIMKQLTELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            3773..5743
                     /gene="etpD"
                     /locus_tag="ACHOT6_003143"
     CDS             3773..5743
                     /gene="etpD"
                     /locus_tag="ACHOT6_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFP9948318.1"
                     /translation="MFRKWLNGRLSVFVFTTVFLSAIPGWGAEFSASFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDASPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVMKRLMEIVERVDKEGNRSVATVPLTYASATDVARLVTELTKES
                     DKTAMPAWMTAKLVADERTNSVLVSGEPISQQRIISMIKQLDRKEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSNIENDAKKSSSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEVQDADGLNLGIQWVNKHSGVTQFTSTGLPITTMVQTR
                     QSDISDSDQSNTLSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFSSKSEHAAKRNLMLFIRPTIIRERDGFRHASAEKYLSFNQEQVQSRGKETMALTL
                     NEEQLKLSRDRDDTTFRKVKTAIAAFYARGMQ"
     gene            5740..7242
                     /gene="gspE"
                     /locus_tag="ACHOT6_003144"
     CDS             5740..7242
                     /gene="gspE"
                     /locus_tag="ACHOT6_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9948319.1"
                     /translation="MSSIQNVSESRPLLPFSFSRTQRILLLREQEGNQVFCMEDTPAS
                     ALLEVRRVAEGPLNVTTLSAEAFEKQLVSCYQRDSDEARQMMADIGNEMDFYTVAGEL
                     PDSEDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRVDGVLREILR
                     LRRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIVL
                     RLLDKNSVSLDLAALGMSPQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSRLN
                     PQDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDSET
                     AQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLCP
                     RCRQSCQASAELAKDMDIPPETTIWQPAGCQHCSFTGYHGRTGLHELLLIDDRIRTAI
                     YQGEGELSITRLAGGRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            7244..8467
                     /gene="gspF"
                     /locus_tag="ACHOT6_003145"
     CDS             7244..8467
                     /gene="gspF"
                     /locus_tag="ACHOT6_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9948320.1"
                     /translation="MALFHYQASDIHGRKRRGVLEADSARHARQLLREQALIPVRLEE
                     KQLHHQHSPRSILRFRPRGVSSAELALLTRQLSTLVAASLPLEEALDALSRQSEKPRQ
                     RDLIAAVRARVLEGHSLATAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALIYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGL
                     TEVCQTWGPWLLLAAATGWIAGRMMLHKPSRRLAFHHLLLRLPIVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMYISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPS
                     MMRHMIASGENSGELDSMLERAADNQDREFSAQMQLALGLFEPMLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNI"
     gene            8498..8932
                     /gene="gspG"
                     /locus_tag="ACHOT6_003146"
     CDS             8498..8932
                     /gene="gspG"
                     /locus_tag="ACHOT6_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9948321.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     VSDLVALESALDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSNIDIFSPGPDGIPNTEDDIGNWTPGHTQP"
     gene            8929..9480
                     /gene="gspH"
                     /locus_tag="ACHOT6_003147"
     CDS             8929..9480
                     /gene="gspH"
                     /locus_tag="ACHOT6_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9948322.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSMQDGLLIGIQVRSDGWTFKVLQRVTAESFPASVGGDVWQGYVWQTWQ
                     PRRAAMEGKLPDDVRLDLQYLRGLQWMPEHNDGTGPDILLLPGGEVTPFSLLFRLVGG
                     EAVTGLQVDENGLMTLFEGEVEL"
     gene            9477..9842
                     /gene="gspI"
                     /locus_tag="ACHOT6_003148"
     CDS             9477..9842
                     /gene="gspI"
                     /locus_tag="ACHOT6_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9948323.1"
                     /translation="MKRQEGMTLLEVMVALVVFALAGIALIQSTTQQAAGIGPMEEKV
                     LAGWLADNQMVQLQLEKIWPKNGWDEKTISFAGREWYLRWHGRDSDIPQQRILEVEVR
                     RTREETSALVSLRGSVVRE"
     gene            9839..10438
                     /gene="gspJ"
                     /locus_tag="ACHOT6_003149"
     CDS             9839..10438
                     /gene="gspJ"
                     /locus_tag="ACHOT6_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9948324.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQSGIRAHELSQD
                     KVRRLAELQRGGSQIERDLMQMIPRHSRGSEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFYVDHPSGIAPDVRVVLEGVHAFRLRFFVNGT
                     WQARWDSTSILPQAVEVTLVMDDFAELTRLFLVSKETAE"
     gene            10435..11412
                     /gene="gspK"
                     /locus_tag="ACHOT6_003150"
     CDS             10435..11412
                     /gene="gspK"
                     /locus_tag="ACHOT6_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9948325.1"
                     /translation="MKSREQGVALLVVLLILSLMVTIAAAIAERNGRTFWRTVAQLDQ
                     LQAKWDGYSAEEMAIQILQRSRQASPRKTHLAQNWAQSELQFETGGGDVRGRIVDAQA
                     CFNLNAINYGAVDLAGVPYAARIFQQLLINLQVDIIQARQVTAALRDWIDRDDEPVKG
                     GAEDEVYMGMEPPYLAASQPMQDVSELRLIRGIDVRLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPNSLLVTKLLLRRPRTGWDSVAAFLDSPQLKGIDTSTAISV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRSIQRQYGLTVREVP"
     gene            11346..12623
                     /gene="gspL"
                     /locus_tag="ACHOT6_003151"
     CDS             11346..12623
                     /gene="gspL"
                     /locus_tag="ACHOT6_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9948326.1"
                     /translation="MERGAFPEHSATVWINGEGGTLNKNNKHVEAGRLFLRLPEDDTQ
                     PLLWRLVSPEETGGGSVAVGETDPLLAGLLPRYPAWVLVPSDRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQTGGRCNVAVVEKSVMHHWLTYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVRLAGQWLFRSDRYAGMTVELSWLTQFLSLTAPPVIESYSV
                     PPAQCMGLETTEWRAQPVRDLLQLAAECEGYDGADLRQGIFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAEFWQHESVRVYQELFPGETVVKDPHRQMLRHLQ
                     QSTSNNMPELGGVMHQLQQLLPETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     SAGEKYIIQPGEIRQQPHGVESRLILRMNNERS"
     gene            12610..13125
                     /gene="gspM"
                     /locus_tag="ACHOT6_003152"
     CDS             12610..13125
                     /gene="gspM"
                     /locus_tag="ACHOT6_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFP9948327.1"
                     /translation="MNDLKKRLQYISPRERRLLTGCVALLVVAFVYYVFWLPWQVRED
                     KWRTVIAREKYTVEWMKQQIPSIKKWEASMPEQGEPLGLSAAVTQTSAAYGVSVTRLQ
                     PQGERLTVTLAPVEFNTLMQWLTQLHRRYRIRTVVFDVVAQGNQPGHVTVNRLVLSRD
                     DINRDTSPGHL"
     gene            13252..14010
                     /locus_tag="ACHOT6_003153"
     CDS             13252..14010
                     /locus_tag="ACHOT6_003153"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFP9948328.1"
                     /translation="MGSFLNVVIHRLPVILERRWRREALMLLAHSEPESEPVFNLMIP
                     RSHCPYCFQPLSIRDNIPLLSYLFLKGKSRCCGERIPIYYPLVEITSSVLFILAASRF
                     HPGLTLVAAWLFIAMLLVLAVIDCRTAMLPDVLTLSLLWLGLLFSLQGGRVPLEEAVV
                     GAVSGYLCLWGLYWLFRFVTGREGLGYGDFKLAAALGAWMGWQVLPSVLLFASVSGLI
                     ITVLLRFVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            14104..14505
                     /gene="eptO"
                     /locus_tag="ACHOT6_003154"
                     /gene_synonym="gspS"
     CDS             14104..14505
                     /gene="eptO"
                     /locus_tag="ACHOT6_003154"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFP9948329.1"
                     /translation="MRINTLKKLNCITSLLVLVTVAGCHQVPSTRKQATVPPSEQLEQ
                     TASIAAATRYLKRKCNRSDLPDDQAILNGVNRIANGKGWQSLTQEDIRKHSDEINERL
                     ARDSTPEHIKCSEFNRLLVPFIGELLAGTSR"
     gene            14631..14795
                     /locus_tag="ACHOT6_003155"
                     /pseudo
     CDS             14631..14795
                     /locus_tag="ACHOT6_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            15094..15387
                     /locus_tag="ACHOT6_003156"
                     /pseudo
     CDS             15094..15387
                     /locus_tag="ACHOT6_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            15616..15864
                     /locus_tag="ACHOT6_003157"
     CDS             15616..15864
                     /locus_tag="ACHOT6_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HFP9948330.1"
                     /translation="MSQTENAVTSSSGTKRAYRKGNPLTLAERQQASLARKRATHKEL
                     RVFIPAALKVQLQEMCEAEGVTQAEMIAELIKQKSAFS"
     gene            15887..15994
                     /locus_tag="ACHOT6_003158"
                     /pseudo
     CDS             15887..15994
                     /locus_tag="ACHOT6_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="RepA; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
     gene            16950..17200
                     /locus_tag="ACHOT6_003159"
                     /pseudo
     CDS             16950..17200
                     /locus_tag="ACHOT6_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
     gene            17197..>17425
                     /locus_tag="ACHOT6_003160"
     CDS             17197..>17425
                     /locus_tag="ACHOT6_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9948331.1"
                     /translation="MTYTVKFRDDALKEWLKLDKSIQQQFAKKLKKCSENPHIPSAKL
                     RGLKDCYKIKLRASGFRLVYQVSDDMLIIAVV"
BASE COUNT         4322 a         3979 c         4832 g         4292 t
ORIGIN      
        1 tattttttat caggttatct ttctgaagta gatataagtg tgttataaaa atcaacactg
       61 gtgaatgagt gaatcactcc tgaaaaaata aaatttaaca cttctattat atccggctgc
      121 atgaaatcta aaatgaaaga tggagtacac ctatgaaatt aaagtatctg tcatgcatga
      181 tccttgcctc tctggcgatg ggagcatttg ctgcaactgc tgctgataat aattcagcca
      241 tttatttcaa taccacccag cccgtaaatg atctgcaggg gggactggcc gctgaggtga
      301 aatttgcaca gagccagatt ttatctgccc atcctaaaga aggggaaagt caacaacatc
      361 tgaccagcct gcggaaaagt ttgctgttgg tccgtctggt gaaggctgat gataaaacac
      421 ctgttcaggt ggaagcccga gatgctaatg ataaaattct cggtacgtta accctttcgc
      481 ctccctcctc actaccggat accgtctacc acctagatgg tgttcctgct gatggtatcg
      541 atttcactcc tcagaatgga acgaaaaaga tcattaatac ggttgctgaa gtaaacaaac
      601 tcagtgatgc cagcgggagt tctattaaaa gctatctggc aaataatgca ctggtggaga
      661 tccagacagc aaatggtcgt tggataagag acatgtattt gccgcaggga gccgagcttg
      721 aaggtaagat ggttcgcttt gtttcgtatg ccggctataa ttcaacggtt ttttatggag
      781 atcgaaaggt cacgctctcg gtgggtaaca ccctgctgtt caaatatgta aatggtcagt
      841 ggttccgctc cggtgagctg gagaataacc gaatcgctta tgctcagcat acctggagtg
      901 ctgaactgcc tgcgcactgg atcgtgcccg ggttaaactt ggtgattaaa caaggtaacc
      961 tgagtggtag cctaaatgac atcaatgttg gagcaccagg ggagctgttg ttgcatacca
     1021 tcgatatcgg gatgctgacc actccccggg ggcgctttga ttttgccaag gacaaagagg
     1081 cgcatcggga atatttccag acgatcccgg tcagtcgtat gatcgtcaac aattatgctc
     1141 cacttcatct gaaggaagtc atgctaccta ccggtacatt gctgacagat gcagatcccg
     1201 gtgagggtgg atggcatagc ggcacgatgc gacagagtat cggtaaggag ctgatctctc
     1261 atggtattga taatgccaac tatggtatta acagcactgc tggttcagga gaaagcagcc
     1321 atccttatgt ggctgcgcaa ctgaccgcac atacaaaccg cggtaactac tccaatggag
     1381 tacaggtgca cggtggttcc ggtggagggg gaattgttac cctagactct accttgggta
     1441 atgaattcag ccatgaagtc gggcataact tcggtcttgg tcattatgtg gggggcttca
     1501 gggggtccgt gcatcgcagc gctgaccaga tcaactcgac ttggggctgg gacagcgata
     1561 aaaaacgttt catacctaat ttttatccag cccagacaaa ccggaaaagt tgtcttgacg
     1621 agcagtgcca ggagccgttc gacggtcact cgtttggctt tgacgccatg gcgggaggca
     1681 gtcctttctc cgacgcaaac cgtttcacaa tgtatacgcc aaattcatcg gctatcatcc
     1741 agcgcttttt tgaaaataaa gctgtgttcg atagcctttc ctccaccgga ttcagcaagt
     1801 ggaatgccga tgagcataaa atggaaccgt atgaacacac cattgacagg tttgagcaga
     1861 ttactgcttc tgtcagggat ctcagtgaaa acaaaatggc tgagatgatg gcagaatatc
     1921 ctattgtcaa agtacatatg tcggacggca actggactag agatattcat gcccctgcag
     1981 cttccgcgga gaataaaggc cgtattctga cactcgatca tgaagctggt tataacagcc
     2041 atctgtttat caacggtggt gaaaagataa tttccaaggg atataagaag agctttgttt
     2101 ccgatgggca gacctggaaa gaaagtgacg tggtgaatac ccgcgaagcg cgtaagccag
     2161 agaagtttgg cgttccggtg acgacactgg tgggttatta cgatcccaaa ggcactttgt
     2221 ctagcttcat ctatcctgca atgtacggtg cgtatggttt cacttattct gatgacagtg
     2281 agaacctgtc cagtaatgac tgccagctgc aggtggatac tagagaaggg cagctacgat
     2341 tcaaactggc taataatcgt gtgaacagtt cagtgatgaa caagttccat atcaacgtgc
     2401 cgactgaaag tcagcccact caggcgattt tagtctgtaa taacaaagta ttggataccc
     2461 gaacactcac accagcacca gaaggtctga cttatactgt aaatggtcgg gcacttccgg
     2521 caaaagaaaa cgagggatgt atcgtttctg tgaagactgc ccagcgttac tgtctccccg
     2581 ttggccagcg gtcaggatcc agtctgccag actggatcgc aggccaggaa gtctatgtcg
     2641 acagcggggc caaagcgaaa gtgctgctct ctgactggga taacctgtcc tataacagga
     2701 ttggtgagtt tgtgggtaat gtgaatccag ctgatatgaa aaaagttaaa gcttggaacg
     2761 ggcagtacct ggacttcagc agaccgaggt cgatgagggt tgtaaataaa taatccggaa
     2821 gacggtgcgg ccaccaggtc gcatcggtaa tacattcgtt ccgttcatca aaacctagaa
     2881 tagcgcccgg tgttttatgt tgttctttct atcatcccgg agagaccgga acttattcat
     2941 aaaagatatc gcacttaaaa tgcttacgcc aaactgggtg ttgtgcgtga ttttactcat
     3001 tgccggatat cagctggtct cggttatacg ccatttctgg ctgactccgg cgacatcggc
     3061 gtccgattta tcccatgtta gcgtgtcgga aacagcggta accgatgagc acacagaaga
     3121 aaattttgtg tttacgttat tcggcacggc atccccgccg ttatcggaag ggaaagtgca
     3181 gaaaacaaca tcttccctgt ctgatgacct gctttcaggg ggggatcttg atgtgagagg
     3241 tatactttac agttcggtta cagagcattc cgttgccata tttgcacata ataacagaca
     3301 gttcagtcta ggcatcggcg aaaaagtacc tggttacgat gctacgatta gtgctatttt
     3361 tagcgatcat attgttatta actatcaggg aaaaaatgca tcactgccgc tgcgatatga
     3421 taatccagca aaaaggaatg cacaggacga taataattta atagtcggac ccgtgacaac
     3481 ccaagctaat tttcgggtaa aaaatatttt tgacataatg agcctttcac ctgtcacggt
     3541 gaataataca ttaagcggtt atcgcttgag ccccggtaag gccagttcgt tattttataa
     3601 tgctgggttg catgataacg atctggccgt attgcttaat ggttcagaac tgcgtgatac
     3661 cagacaggcg aagcagataa tgaaacaatt gacagaactt aaagaaataa aaataaccgt
     3721 cgaacgtgat ggtcaactgt atgatgcctt tattgctgta ggagagaact gattgttcag
     3781 gaaatggttg aacgggcgct tgtcggtatt tgtattcacg acagtatttc tgagtgccat
     3841 tccgggatgg ggggctgaat tttcggccag ctttaaagat acggatattc aggagttcat
     3901 taatactgtc agcaaaaatc tgcacaaaac ggtaataatt aatcctgatg tgcaggggac
     3961 catcactgta cgcagctacg atatgctgaa cgaggaacaa tattatcagt tctttctcag
     4021 cgtgctggat gtttatggtt ttgccgtggt cgatatgcac aacggtatac tgaaagtggt
     4081 gcgttcaaaa gatgctaaaa cgtcggcggt gccggtagct agtgatgcca gccctgggac
     4141 tggtgatgag gttgttaccc gggtggtccc cgtcagtaac gtggcagcca gagacttggc
     4201 gcccttgctg cgtcagctca atgataatgc tggcgcagga agcgtagtgc attatgaacc
     4261 ctctaacgtt ctgctgatga ctggtcgtgc tgccgtgatg aaacggctga tggagattgt
     4321 tgaacgggtg gataaggagg gcaatcgcag cgttgccacg gtcccactta cctacgcctc
     4381 tgcaacggat gtagccagac ttgtcacgga actgactaaa gaaagtgata agacagccat
     4441 gccagcctgg atgacagcga agctggttgc tgacgagcgg acaaactcag ttctcgtcag
     4501 cggagagcca atctcccaac agcgtatcat ctccatgatt aagcaactgg accgtaagga
     4561 ggatgttcag ggtaatacga aggtaattta cctgaaatat gcgaaggcaa aggatttagt
     4621 ggaagtcctg acgggcatca gcagcaatat tgaaaacgac gctaaaaaga gctcgtcaac
     4681 ggaagctctg cgaaaagggg tgacgattaa atcccacgaa caaaccaatg ccttgatcct
     4741 gacaggggct cctgacgtca tccgcgactt ggaaaatgta atttcgcagt tggatattcg
     4801 tcgtcctcag gtcctggtgg aagccatcat tgctgaagta caggatgctg acgggttgaa
     4861 tctggggatt cagtgggtga ataaacactc cggtgtgacg cagttcacca gtactggttt
     4921 acccattacc acgatggttc agacccgtca gagcgatatt tctgacagcg atcagagcaa
     4981 taccttgagc atgtttaacg ggattgcagc agggttttat cagggaaact gggcgatgct
     5041 gctgacggcg ctctccacaa gtagcaaaaa tgatatcttg gcaaccccca gtattgttac
     5101 gctggacaat atggaggcca cgttcaatgt tggtcaggaa gttccggtgc tttcgggttc
     5161 acagaccacc tctggggaca atatttttaa caccgtcgag cgtaaaacgg tggggatcaa
     5221 actcagggta aaaccccaga tcaacgaggg tgattcagtg ttactggaga tagagcagga
     5281 ggtgtccggt gtggcagata cagcagtagc taccactact gacttggggg caaccttcaa
     5341 tacccggaca gtgaccaatg ctatgctggt agggaatggc gaaacggtgg tggtcggcgg
     5401 attactggat aagtcgatca gagggagtga gagtaaagta cctctgctgg gggatatccc
     5461 agtactggga catcttttta gctcaaaaag tgaacatgca gccaagcgta atctgatgtt
     5521 gttcattcgg ccaacaatta ttcgtgaacg cgacggattt cgtcatgctt cggccgaaaa
     5581 atacctgtcg tttaatcagg agcaggtgca gtcgcgtggt aaagaaacaa tggcgctgac
     5641 gctgaatgag gaacagctca agctatcccg agatcgggac gatacgactt ttcggaaagt
     5701 gaaaacggcg attgctgcgt tttatgcgcg ggggatgcaa tgagcagtat acagaacgtc
     5761 agtgaatcac gcccccttct ccctttttcc ttttcccgta cacagcgaat tttgttgctg
     5821 cgtgaacagg aagggaacca agtgttttgc atggaggaca cacctgccag tgccctgcta
     5881 gaggttcgca gggtagctga aggtcctctg aatgtcacaa ctctgtcggc tgaggcattt
     5941 gaaaaacagc tggtgagctg ctatcagcgt gattctgatg aagcgcgaca aatgatggct
     6001 gatattggta atgaaatgga tttttatacc gtcgcggggg agttgcctga cagtgaggac
     6061 ttactggatg cgaatgacga tgcgcctatt atccgcctga ttaacgccat gctgactgag
     6121 gcaattaaag agaaagcctc agatattcat attgaaacgt acgaacgcca tctacaggtt
     6181 cgctttcgcg ttgatggcgt tctccgggag atcctccgtt tacgccggaa tctggcttcg
     6241 ttactgattt cacgcattaa agttatggct cggctggata ttgccgaaaa acgcgttccc
     6301 caagatggtc gcatggtgct gcgtataggc ggacgggctg tggatgtgcg tgtttcaacg
     6361 ttgccttcga accatggtga acgcatcgtg ctgcgtttac tagacaaaaa cagcgttagt
     6421 ctcgatctgg ctgcactcgg catgtcgccg cagaatcaga gacacattga tgcgctgatc
     6481 cgccgtcctc acggcattat tttggtgact gggccaactg ggtcggggaa aagcacgacg
     6541 ctttatgctg cgttaagccg gctgaatcca caggaccgca acatcatgac agtagaggat
     6601 cctgttgaat atgaactgga cggtatcagt cagacccagg tcaacccgaa ggtggacatg
     6661 acttttgcca ggagcctgcg tgctatttta cgtcaggatc cagatgtggt gcttgtaggg
     6721 gagatccgtg acagtgaaac agcccagatt gctgtacagg cctcactgac tgggcatctg
     6781 gtgctgtcaa cgttacatac gaacagcgct gcaggcgcgt tatcgcgcct gcaggatatg
     6841 ggcatccccc cctttctgct ttccacctca ttgctggctg ttctagccca acgactagtt
     6901 cgcacgctgt gtccgcgctg tcgtcagtcc tgtcaggcca gtgctgagct ggcgaaggac
     6961 atggatattc ccccggaaac cacgatttgg cagcctgccg ggtgtcagca ctgcagtttt
     7021 actggctatc acgggcgtac cgggctccac gaactgttgc tgattgacga tcgcatccgg
     7081 acggcaatat atcaggggga aggagagctg agtattaccc gcttggctgg ggggcgctat
     7141 ctgacgctgc gtggcgacgg gcgacaaaag gtgctagccg gtgagaccag ttgggaggag
     7201 gtggttcgcg ttactgaaag caggttgcag gaagaggaat gatatggcgt tgtttcatta
     7261 tcaggcatcg gatattcacg gtagaaaacg ccgtggcgtt ctggaggcgg attcagctcg
     7321 acatgcccgc cagttgctgc gtgagcaggc gcttattcca gttaggctgg aagagaaaca
     7381 gctccatcac cagcactcac cgcggagtat ccttcgattt cgtcctcgtg gggtgagcag
     7441 cgccgagctt gcattattga cacggcagtt atccaccttg gtggccgcat cactgccgct
     7501 tgaagaggcg ctggacgcgc tgtcgaggca gagtgagaaa cctcgccagc gggatttaat
     7561 cgctgccgtg cgcgctaggg tccttgaggg acattccttg gccacggcga tgggaatgtt
     7621 tcctggtacg tttgagcgtc tttattgtgc gatggtggcg gcgggtgaaa catccggtcg
     7681 cttggatgtg gtacttagtc gtctggctga ctataccgag caacgccaga tcatgcgaaa
     7741 ccgtctgctc caggcactga tctatccctg tgtcctgacg ttggtagcgg taggggtgat
     7801 tgccattctg cttactgctg ttgtgccgaa agtggttgag cagtttattc atatgaaaca
     7861 gacccttcct ctgtctaccc gcgtactgat ggggctgact gaggtctgcc agacttgggg
     7921 cccgtggctg ctgctagccg cagccacggg ctggatagcg gggcgtatga tgttacacaa
     7981 gccctcccgg cgtctggctt ttcatcatct gctgctgcgc ctgccgatcg tggggcgtat
     8041 atcgcgtggg ctgaataccg cacgttatgc ccgcacgctg agcatcctta atgccagtgc
     8101 ggtgccgcta ctccaggcta tgtacatcag tggagatgtg ttgagtaatg actgggcccg
     8161 tcatcagtta gccaccgcag ccgaattggt tcgggaaggg gtcagcctgc atcaggcgct
     8221 ggagcagacc tcgctatttc cgtccatgat gcggcacatg attgccagcg gtgaaaatag
     8281 tggcgaactc gacagcatgc tggaacgggc cgccgataat caggatcgtg aattcagcgc
     8341 acagatgcag ctggcgctgg gattgtttga accaatgttg gtggtgggga tggctggcgt
     8401 cgtcttgttt attgttctgg caattctgca accgctcctg caactcaaca atatgatgaa
     8461 tatttgaatt cttctttaat agtcaacgga gaaaaacatg cgaaaacagc atcagcgtgg
     8521 ttttaccttg ttggaaataa tggtggtaat tgtgatcttg ggagttctgg ccagtctggt
     8581 ggtcccgaat ctgatgggaa ataaggataa ggccgatcgg cagaaagtgg ttagcgatct
     8641 ggttgcgctg gaaagcgcgc tcgatatgta tcggttagat aacaaccgtt acccaacaac
     8701 ggagcagggg ctacgggcac tggtcagtaa acctacagtg cagcctgagc cccgtaacta
     8761 tcggcaagat ggttatatac gtcgtttgcc tcaagatccc tggggggggg attatcagct
     8821 gttaaatcct ggtcagtaca gcaatattga catcttctct cccggcccgg atggtatacc
     8881 gaacacggaa gatgatattg gtaactggac gcccggtcac acgcagccgt gagtcagcgc
     8941 ggattcacaa tactggagat gatgctgatc cttttgttga tgggcattac tgcagggttg
     9001 gtactaatgt catttcctga ctcagcgcag aatcatttgc aacaacagcg ggagcgtctg
     9061 caggcgcagc tggattatgc cttggataga agcatgcagg acggcttgct gattgggatc
     9121 caagtccggt ctgacgggtg gacatttaag gttttgcagc gcgtaacagc tgaaagcttc
     9181 ccggcatcag tgggggggga tgtttggcaa gggtatgtct ggcaaacttg gcaaccgcgg
     9241 cgtgctgcca tggagggcaa acttccggat gatgtgcgtc ttgatctgca gtatctcaga
     9301 ggtctgcagt ggatgcctga gcataatgac gggacagggc ccgatatttt gttgctgcct
     9361 ggaggtgagg tgacgccgtt cagccttctc tttaggctgg taggagggga ggccgttact
     9421 gggctgcagg ttgatgagaa cggcctgatg accctatttg aaggtgaggt cgagttgtga
     9481 agaggcaaga aggaatgaca ttactagaag tcatggtggc cttggttgtg tttgcgctgg
     9541 caggaattgc tctaattcag tccacgactc agcaggccgc agggatcggg ccgatggaag
     9601 agaaggtgtt ggcaggctgg ttggctgaca accagatggt gcaactgcag ctcgagaaaa
     9661 tctggccgaa aaacggctgg gacgagaaga cgatcagctt cgctggcaga gaatggtatt
     9721 tacgctggca cgggagggac agcgacatac cacagcaacg catcctagag gtagaggtac
     9781 ggcgtaccag agaggagacg tccgccttag tttccttgcg tggtagtgtg gttcgtgaat
     9841 gagtcagcag cgggtgaaag gttttacgct actggaaatg ctgttggcac tggctgtttt
     9901 tgcagcgctg agcatcagcg catttcaggt actacagagc ggaatacgcg cgcacgagct
     9961 gtcccaagat aaagtacggc gtttggctga gctgcagagg gggggcagcc agatagagcg
    10021 agatcttatg cagatgatcc caagacatag ccgggggagt gaaggcctgc tgctcgctgc
    10081 tcctcatctg ctgaaaagcg atgactgggg gatttccttt acgcgaaaca gttggcttaa
    10141 tcccgccggg atgctaccac gcccggagtt acagtgggta ggttaccggc tgcgacagca
    10201 aaagttagaa cgcctgagct acttttatgt cgatcacccc tctggtattg ctccggatgt
    10261 cagggtggtg ctggaggggg ttcacgcatt tcgtctgcgt ttttttgtca acggcacttg
    10321 gcaggcgcgc tgggacagta ccagcatcct gccgcaggct gttgaggtga cgctggttat
    10381 ggatgatttt gctgaactta cccgtctgtt tcttgtaagt aaggagaccg cggaatgaag
    10441 tcgcgggaac agggcgtggc gcttctggtt gtattgctga tcttgtcgtt aatggtgacg
    10501 atcgcggctg cgatcgcgga gcgtaacggc aggacatttt ggcgtacggt ggcgcagctg
    10561 gatcagctgc aggcaaagtg ggatggctat tctgcagagg agatggcaat acaaatatta
    10621 cagaggagca gacaggcatc gccccggaaa acgcatctgg cacaaaactg ggcgcagtca
    10681 gaactacagt ttgaaaccgg cggaggagat gttcggggac ggatagtgga tgcacaggcc
    10741 tgttttaatc ttaatgccat taattatgga gcagtggatc tagccggtgt gccttatgcc
    10801 gccagaattt ttcagcagct gttaatcaac ctgcaagtcg atattataca agccaggcaa
    10861 gtgacagcag ctctgcgtga ctggatcgat cgtgatgacg aaccagttaa ggggggggct
    10921 gaagacgagg tgtatatggg gatggagccc ccctatcttg cggccagcca accgatgcag
    10981 gacgtcagtg agttgcgcct catccgggga atagatgtca gactttaccg caaactttta
    11041 ccgtatgttt gtgtactgcc cacgtcagac ctgtcagtga atgtgaatac tctgctggat
    11101 tcacaggcgc cgttgctggc tgcactcttt ctgacgaaac cgaacagtct tttggtgacg
    11161 aaattactgc tgcggcgacc ccgtaccggt tgggatagcg tggcagcgtt tcttgactcg
    11221 ccacagctta agggcatcga caccagtaca gccatatcgg tactggctgt tagcagcaac
    11281 tattttttgg tccggttaca tgtgcgctct ggggagcacc tgttcagcca gcagacttta
    11341 atgcagtgga gagaggagcg tttccggagc attcagcgac agtatggatt aacggtgagg
    11401 gaggtaccct gaacaagaat aacaaacatg tcgaggcagg ccggcttttt ttgcgactgc
    11461 cggaggatga cacgcagcct ttattgtgga ggttggtttc gccggaggag acagggggcg
    11521 gcagcgtggc agtcggagaa acagatccgc tgttagcagg tctgctgcct cgttatcccg
    11581 cttgggtgct ggttccgtca gacagaattg ccttccatca ggtcacgctg ccacaccgta
    11641 cgtggcgtca gcgtttgcag gtgctcccct ttcttctgga agagcagttg gcaacagata
    11701 ttgagcagct gcatttcgcc attcttcatc agacaggtgg caggtgtaat gtggctgtgg
    11761 tcgaaaaatc ggtcatgcac cactggctga cgtactgtgg acaacttggg gtccgggagg
    11821 tgaggttgct gcctgacgta ctgatgctgc cgttggcaac tgaaggatgg agtgcagtaa
    11881 ggcttgccgg tcagtggcta tttcgtagtg atcgttacgc cgggatgacg gtggagttgt
    11941 cgtggctgac acagttcttg tctcttactg cgccgccggt tattgagtct tactccgtgc
    12001 cgccagcaca gtgcatgggc ttggagacca cggagtggcg agctcagccc gtacgggatc
    12061 tgctgcagtt ggcggctgaa tgtgagggat atgatggcgc agatctgcga cagggaatat
    12121 ttgctcgcca gggaacatgg tatgcgcgat tccgcccatg gcggtatgtc gcaggggccc
    12181 tgttggcctg tgtgtttctg gccggaagca acgcggggct ggcacattac cggctctggc
    12241 agcaggctga attttggcag catgagagcg tacgcgtcta tcaggagctg tttcctggcg
    12301 aaacggtggt gaaggatccc caccggcaga tgctgagaca tctgcaacag tcgacgagta
    12361 acaatatgcc ggaactaggg ggcgtgatgc accagcttca gcaactgctg ccagaaacct
    12421 caacgatacg cttacaggca ctcgcctggg acagtagcag ccagactctg aaaatagatc
    12481 tgcaggcagc ctcttttcag gctctggaac actttcagca gagcgctgga gaaaaatata
    12541 tcatccagcc tggcgagata cggcagcagc cgcacggggt ggaaagtcgt ctgatcctga
    12601 gaatgaacaa tgaacgatct taaaaaacgt ttgcagtata ttagtccccg tgagcgaagg
    12661 ctgcttaccg gatgtgttgc cttacttgtt gtcgcttttg tctattatgt cttctggctg
    12721 ccctggcagg tacgggagga taagtggcgg actgtaattg cccgtgaaaa atacacagtg
    12781 gagtggatga aacaacagat acccagcata aagaaatggg aggccagtat gccagaacag
    12841 ggggagccgt taggtttatc tgcggcagtg actcaaactt cagccgcata tggtgtatca
    12901 gtcacccgat tgcagcctca gggggagcgt ctgaccgtta ccttagcacc agttgaattt
    12961 aatacgctga tgcagtggct tacgcaactg catcgcaggt accgtattcg taccgtggta
    13021 tttgatgtgg tcgcgcaggg aaaccagccc ggacatgtaa cggttaatcg gctggttttg
    13081 tcccgggatg acataaacag ggatacttcg ccggggcact tgtgatttac aggttaatgc
    13141 ctgacgtgaa aatgggagtg ataagcagga ggatatgtga gcaggttgcc ggaatttgcg
    13201 gctgattttc atttgttgtg gctcatcagc acaggggggc tagggggcat tatcggtagt
    13261 tttcttaacg ttgtgattca tcgtctgccg gtaatactgg agaggcggtg gcggcgtgaa
    13321 gcgctgatgt tgttggcaca ttctgagcct gagtccgagc cggtctttaa cctgatgata
    13381 ccgcgctctc attgtcccta ctgttttcag ccgctcagca tcagggataa cattcccctg
    13441 ttaagctatc tctttctgaa ggggaaatca cgctgttgtg gtgagcgtat tcctatttac
    13501 tatccgctgg tcgagataac gagttcagtg ttattcattc tggcagcgag ccggtttcat
    13561 cccgggctga cattagttgc tgcctggctt tttattgcca tgctgttggt tctggcggtt
    13621 attgactgtc gtacggctat gctgccggat gtgctgacgt tatccctgct ctggcttggc
    13681 ctgttattca gcttacaggg ggggagagtg cccttggagg aggcggtagt gggggcggtc
    13741 tctgggtatc tctgtctgtg ggggctgtac tggctttttc gtttcgttac cggtagagaa
    13801 gggttaggct acggtgattt taagctagct gcggcactgg gagcatggat ggggtggcag
    13861 gtattaccct ccgtgcttct gtttgcctca gtcagcggat taatcataac ggtactgctg
    13921 cgttttgtta cagccacgga ttttacccgg ccgctgcctt ttggtccgtg gctcgccttg
    13981 agcggagtat gccatttttt gttgatgtag cgttcggttt ccggggctgg taataaaaat
    14041 ggctcagaga ataacgggac tatagtaaac cggaaaccgg ttaaacaata cctggttaaa
    14101 aatatgagga ttaatacgtt gaaaaaacta aattgtatca cgtcattact ggtcctggtg
    14161 actgtcgccg gttgccatca ggtaccttcc actcgtaagc aggccaccgt tcccccgagc
    14221 gaacagctag aacaaacggc gtcgatcgcc gctgcgacac ggtatctgaa aaggaaatgt
    14281 aaccgcagtg atttgccaga tgatcaggcg atactgaatg gcgttaaccg gatcgctaac
    14341 gggaaagggt ggcagtcctt aactcaggag gatatacgta aacacagcga tgagatcaat
    14401 gaacgtctgg ctagggacag cacacctgag cacataaaat gcagtgagtt caatcggctg
    14461 ttggtcccat ttatcggtga attactggca gggactagca gataataggg tactgacagg
    14521 tactgaaccg cgcctcggaa atctaggaga ctaaactccc tgagaaagag gtaaacagga
    14581 tgaccaaaaa tacacgtttt tccccggaag tccgttagcg gacggtccgt atgattctgg
    14641 tgcgtcaggg cgaatatgac ttacaacggg cggcaatttg tttcattgcc ccaaagatta
    14701 gctgtacgcc agaaactcta cgtgtctggg ggcgccagca tgagcgggct accgggagcg
    14761 gtaatgatgg actaactgcc actgagcgcc agcaaagtga aggccataag ctagaggttc
    14821 atgatatacc ccagtggctt ccatgatgat atgaacttcg ttcagttcac tggattgctt
    14881 tttaagccac tctgccacag ttttagccgc atcagcggta ttttttagct tacgagtttt
    14941 gatcttccct ttaatgtccc ccgtaagagg catagatcaa gattgttttt actgacatca
    15001 atacctaact gaaacatcgg cattttctcc tctttcacat aaccatcacg ccaatttgtc
    15061 ttgttcatgt atacggcatc ggtgatgcag agtgaatatt ggtgaggtca gaagacgctg
    15121 ctgacagaaa gggaagtcag ttttatgaaa ggactgtcca aaatcgtgga tatgaaatgg
    15181 tggtatctgt gtccgcaggt acgggctgat atcgtccgtt gaacggcgct cgttcagtat
    15241 tgttgcagca gtagagctgg atgatacctg tcattttcga ctggaacgct ggcgccggga
    15301 tattagtctg gaagaggtta tgaggctggc attccgttgc tcagaagtca cgatgccaaa
    15361 aaactgctgc agatgaccgg agaatagctg gataccttca gaacagagct ccagtcagaa
    15421 tcgggatgca gcaagacccg gcattttcat acctgaaaac catactattt cacagtttaa
    15481 atcacattaa acaacagtaa ttcccattga tttgtgcgcc aacacagatc ttcgtcacaa
    15541 ttctcaagtt tttgatttca aaaaactgta gtatcctctg cgaaacgatc ccggttcaaa
    15601 tattgaggag gcgagatgtc gcagacagag aatgcagtaa cttcctcatc aggtacaaag
    15661 cgagcatacc gaaaaggtaa tcctctgacg cttgcagaac gtcagcaggc gtccttggca
    15721 agaaaacgtg ctacacataa agaactcaga gtattcattc ctgctgcgtt aaaagttcaa
    15781 ttacaggaga tgtgtgaggc tgaaggcgtt actcaggctg agatgattgc tgaactgata
    15841 aagcagaaga gtgctttcag ttaactaaaa tgtagcgaca tcacattctt gcaatcgtga
    15901 ggcggtaaaa cgcgaagtgg agcgtcgtgt gaaggagcgc atgattctgt cacgtaaccg
    15961 taattacagc cggctggcca cagcttcccc ctgaaagtga cctcctcaga ataatccggc
    16021 ccgcgccgaa ggcatccaca cgcctgaagc ccggcagcgc acaaaaaatc agcaccacat
    16081 acaaaaaaca acctcaccat ccaccttctg gagcatccgg ttccccctgt tttcgataca
    16141 aaacacgcct cactgacggg taattttgct tatccacatt aaactgtaag ggacttcccc
    16201 ataaggttac aaccgtttat gtcataaagc gccagccgcc agccttacag ggtgcaatat
    16261 atcttttaaa catctgttta tatctccttt aaactactat ttaaaaagaa aacctattca
    16321 ctgcctgtcc tgtggacaga cagatatgca cctcccaccg caagcggcgg gccccgaccg
    16381 gagccatttt agttacaaca ctcaaataca accaccagaa aaaccccggt ccagcgcaga
    16441 accgaaacaa caaagcccct ccctcataac tgaaaagcgg ccccgccccg gcccgaaggg
    16501 ccggaacaga gtcgctttta attatgaatg ttgtaactat actccgtcat cgctgtcagt
    16561 gttctggctg gaagtcctca gtacacgctc gtaagcggcc ctcacggccc gctaacgcgg
    16621 agatacgccc cgacttcggg taaaccctcg tcgggaccac tccgaccgcg cacagaagct
    16681 ctatcacggc tgaaagcagg tatggtctgg cagggccggg gatgggtaag gtgaaatcta
    16741 tcaatcagta ccggcttacg ccgggcttcg gcggttttac tccggtatca catgtaaccg
    16801 cagagtgccg ccttccatgc cgctcacgcg gcatcagagc aatacaacca gcattaaatt
    16861 catttgcaaa tgtatgagta atacagactt gttaaacggt cctttcagag gtatctttaa
    16921 gacttgttaa acagtcttaa aggtgcatta tgcctgacat cattctgtct gacaccagtg
    16981 caagtgtcag tgaactgaaa aaaaatccta tggctacagt caatgccgaa gatggttact
    17041 ctgtcgccat tcttaatcag aatcagccgg catctactgt gtgcctgctg aactctacga
    17101 aaggatgcct gatgctctgg atgaccagga actggtgaaa ctggttactg aacgcagcaa
    17161 tcagccactg tatgatgtgg acccgggccg ttatttatga cttatacggt aaaattcagg
    17221 gatgatgcgc tcaaagaatg gctgaaactg gataaatcca ttcagcagca gttcgcgaaa
    17281 aaactaaaga aatgcagcga aaatcctcat attccttctg caaaattacg tggactaaaa
    17341 gattgctaca aaatcaaact tcgcgcatcg gggtttcgtc tggtatatca ggtgagtgat
    17401 gacatgttaa ttattgctgt tgtta
//
LOCUS       DAZKIV010000099        17213 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZKIV010000099 DAZKIV010000000
VERSION     DAZKIV010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17213
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.099"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..491
                     /locus_tag="ACHOT6_003161"
     CDS             <1..491
                     /locus_tag="ACHOT6_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFP9948332.1"
                     /translation="FAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTDNTAL
                     PKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPLTVTW
                     GGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYHGVVSIYLSQ
                     DI"
     gene            583..3266
                     /locus_tag="ACHOT6_003162"
                     /pseudo
     CDS             583..3266
                     /locus_tag="ACHOT6_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
     gene            3263..4351
                     /locus_tag="ACHOT6_003163"
     CDS             3263..4351
                     /locus_tag="ACHOT6_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFP9948333.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4439..5737)
                     /gene="exuT"
                     /locus_tag="ACHOT6_003164"
     CDS             complement(4439..5737)
                     /gene="exuT"
                     /locus_tag="ACHOT6_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFP9948334.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6220..7632
                     /gene="uxaC"
                     /locus_tag="ACHOT6_003165"
     CDS             6220..7632
                     /gene="uxaC"
                     /locus_tag="ACHOT6_003165"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFP9948335.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7647..9134
                     /gene="uxaA"
                     /locus_tag="ACHOT6_003166"
     CDS             7647..9134
                     /gene="uxaA"
                     /locus_tag="ACHOT6_003166"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFP9948336.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLLIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9217..9768
                     /locus_tag="ACHOT6_003167"
     CDS             9217..9768
                     /locus_tag="ACHOT6_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFP9948337.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWGIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(9773..11017)
                     /gene="sstT"
                     /locus_tag="ACHOT6_003168"
     CDS             complement(9773..11017)
                     /gene="sstT"
                     /locus_tag="ACHOT6_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFP9948338.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(11416..12381)
                     /gene="alx"
                     /locus_tag="ACHOT6_003169"
     CDS             complement(11416..12381)
                     /gene="alx"
                     /locus_tag="ACHOT6_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFP9948339.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     THEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELRDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(12664..13650)
                     /locus_tag="ACHOT6_003170"
     CDS             complement(12664..13650)
                     /locus_tag="ACHOT6_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFP9948340.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(13729..14412)
                     /locus_tag="ACHOT6_003171"
     CDS             complement(13729..14412)
                     /locus_tag="ACHOT6_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFP9948341.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(14489..14992)
                     /locus_tag="ACHOT6_003172"
     CDS             complement(14489..14992)
                     /locus_tag="ACHOT6_003172"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFP9948342.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15077..16213
                     /gene="rlmG"
                     /locus_tag="ACHOT6_003173"
     CDS             15077..16213
                     /gene="rlmG"
                     /locus_tag="ACHOT6_003173"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFP9948343.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLEING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            16498..16812
                     /gene="higB"
                     /locus_tag="ACHOT6_003174"
     CDS             16498..16812
                     /gene="higB"
                     /locus_tag="ACHOT6_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HFP9948344.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            16809..>17213
                     /locus_tag="ACHOT6_003175"
     CDS             16809..>17213
                     /locus_tag="ACHOT6_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9948345.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPAL"
BASE COUNT         4524 a         4287 c         4398 g         4004 t
ORIGIN      
        1 tatttgcaaa atctcttctg gtcgcagcga tgttttctgt tgcaggctcc gcattggcag
       61 tacaaaagga tattactgta acggccaacg ttgatgccgc tctggatatg acacagaccg
      121 ataacaccgc gttgccgaaa gcagtagaaa tgcaatatct gccggggcag ggtctgcaat
      181 cttaccagtt gatgaccaaa atctggtcta acgacgttac caaagatgta aaaatgcagt
      241 tagtttcccc ggcgcaactg gttcaaagcc tggatgctag caagattgtt ccgctgacag
      301 taacctgggg cggtgaagaa attaaagctg atgcggcaac cacttttacc gcaaccaaaa
      361 tctttgcttc cgatgcgtta actaacggtt ctctggctaa aaacctgatg tttgctcaaa
      421 ccaccaaagg tgttctggag acaggcatct accatggtgt agtaagcatt tatctgtctc
      481 aggatatcta atccagataa cagaattaac acgtaacaaa gggaggggta gccctccctt
      541 attttaactg ttttagtatt tgttatggat ttcaggattg ccatggataa aaaattactg
      601 gctcttttga tcctggcgag tctcagcccg gcagaggcga cattaaccaa aattcccgca
      661 gggtttgagg ttattgctca gggacagcag gagtatatcg aggtttattt ttcagggaaa
      721 agtctcggta aatattatgc aatggttaat cttgataccg taacatttct tgatccagca
      781 agtctatata acaagctgga actggatgta gacgatcaga aaatcgcgca tatagtgaaa
      841 gaaaaattat cgcagccgct agctcgccac ggtgaattgg cttgcggtta tgtacgtact
      901 gactcagggt gtggttttct gaataccgat acgctggaaa taatctataa tgatgaagaa
      961 agttcggcaa cgttgtttat taatccgcaa tggaattcag ctttcgatgc gaagtcatta
     1021 tatttaaatc cagacaaaaa tactgttaat gcttttatac atcagcaaga catcaatgtt
     1081 ctggcacagg atgattacca atcgttgtct attcagggaa acggtgcgct gggaataaca
     1141 gaaaatagtt atattggtgc acactggaat ttcaacggtt atgatgcaga tgatgtcagt
     1201 gacagtaatg ctgacgtcag cgatctctat tatcgttatg attttttacg tcgttattat
     1261 gtgcaggcgg gtcgcatgga caaccgcaca ctatttaatg cacaaggcgg gaactttacc
     1321 tttaactttc tgccactcgg tgcaattgat gggatgcgta tcgggtcgac actgagctat
     1381 ttaaaccagg cgcaaagcca gcaaggaacc ccagtaatgg ttctgctttc gcgcaattct
     1441 cgtgttgacg cttatcgtaa tgagcaactt ttgggatcgt tttatctcaa tagtggttcg
     1501 caatttattg ataccagttc ctttccgcca ggcagctaca gcgtggcatt aaaagtctac
     1561 gaaaataacc aactcacccg caccgagctt gtgccgttta ccaaaaccgg cggtctgact
     1621 gacggaaatg cgcaatggtt cttacaggca ggtaaaacta catcacaggt ttctgatgat
     1681 gaaagttcag cttatcagct aggagtacgc ctgccattac atccgcaata tgagctctac
     1741 gcagggctgg cgaatgccga tgatgtgagt gctttcgagt taggtaataa ctggacggca
     1801 gatttaggcg ggtggggaat cttgcaatca gcgccagcgt gttccgtaac gatgacggcg
     1861 gcaaaggtga tatgcaacag gccaactgga gtaatccggg atggccgacg ttgggctttt
     1921 atcggaccaa ctctgacggc gatgcttgta caaccgacag cagagagagc tataacgcct
     1981 taagctgtta tgaaagtatt tccgcgacgg tttcacagaa ttttgtcggc tggaatatga
     2041 tgctgggtta tacccgcaca caaaataaca ctgatgatag tttgcgttgg gataaacagc
     2101 agagctttga aaataactat cttcgccaga caacagcgca aagtatctct gaaactgtac
     2161 aacttagcgc ttcccgcgct tttgtgatgc gtgactggat tttgagtacg tcagtcggtg
     2221 ttttccatcg taatgacaac ggtggcgata acgacgacaa cggcttgtac ttatcgtttt
     2281 cgttatctga cacgccaacg atggatagca ataacaacag ccattcaaca aatgtgtcta
     2341 cggattatcg ttatagcgaa caggatggcg atcaaacgtc atggcagtta tcgcatacct
     2401 tttataacga ttcattcagc cataaagaac ttggcgtaac cgtcggaggc ctgaacaccg
     2461 ataccataaa cagcgcggtt aacgggcgtt gggatgggca atacggaaat gtctacgcta
     2521 ccgtatctga tagttatgac cgtaagaatc atgatcatct ttcggccttt acgggcactt
     2581 atagctctac gctggctgtc agtcgctatg gcgttaattt gggagccagt ggtacagacg
     2641 atttgctggg tgcggtactg gtggatgtga aaggcttctc tgaacaggat gaagagagtc
     2701 aggatctgca actcgaagcg cgggtggcag gcagccgaac tttgcagctt ggtcaaagtg
     2761 acagtgtgtt gttcccttat cttggatttc agtctggttt tgttgaggtt aacgacagta
     2821 gccagggcaa tcagcagggg acaacaaaca tcattaacgg tgcagggaat cgtgaattaa
     2881 tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc cagctttaac tacaactata
     2941 tcggtcgttt attactgccg gcagcggtga aaaaattccc gattgtgggg ctgaacagcg
     3001 ccatgttact ggtagctgaa gatggcggat ttacacttga aattaacggc agcgaaaaag
     3061 agctctatct gctttccggg cagcaattcc ttaagtgtcc actgagtgtt gtaaagaaac
     3121 gcgccagcat tcgttacagc ggagatgtta cttgtagtgt ggtgacttat tcacaattac
     3181 cggagtcgat tcaggttcag gcacagttga aacagcctaa attacgtgga aacgttcaga
     3241 cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat tgggcttgtt
     3301 tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta cgtatgattt
     3361 ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag gaaataatgg
     3421 atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag ctaatggaaa
     3481 tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt ggccggtaaa
     3541 actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgccag gatgcaaggg
     3601 gggcaaaggg cttcagacga atcttaagga ttctaataga tcttcatgta cagaagatgg
     3661 tcaacattat tatatatacg atacaaagtt tcttacgctc taccttgagc agacagagat
     3721 gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt aaattacatt cgaacagccc
     3781 ggcccaggac tatttcgcaa atattaccct gaatacgctc gaccccaacc atattgacgt
     3841 gttcttcccg gagttcgccc acgccacgcc aagggtgcag ttagacttgc atccaacagg
     3901 aagcgttaac ggcagcaact acgcgcaaga tctgaccatg ttggacatgt gcctgtacga
     3961 tggttttaac ggtaatgcca tcagttatga aatcatgctc aaagatgaag ggcgaccagc
     4021 tgcagggcgc agagacggtt acttctctat ctatcgtcag ggagggacca ccaccgacga
     4081 gggagaacgc attgattacc gggtcaaaat gtacaacccg gaaaccggtg ggcaaattga
     4141 tgtgcgcaat aatgaaaata tggtctggaa cagcattaac ctgaaacgtg tgcgtccggt
     4201 ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg acacccttga ctctggcagt
     4261 cgataaattc agcgtgatgg ataaacaggc cgggtactac atgggcaaat tgtcagtaat
     4321 ctttacgcct tccttgccaa ccatcaatta accctgcaaa ccggacaaac gcttttttct
     4381 atataaacga cgaagccgtc tgataaagcg gcttcgtcat tacaacttaa gttaaacgtt
     4441 aatgttgcgg tgcgggatcg ttatgtgttt cctgcgccac ctcaatcgcc ggtttgttct
     4501 gcaacacggt ccagataacc agcgcaccta acaggtcgaa cactgccaga actgcgaaca
     4561 gcgggctgaa gccgatggtg tcagccagtg caccgacaac cagcgcaaac agcgtacttg
     4621 ccagccatgc ggacatcccg gttaaaccgt tcgccgtcgc cacttcgtta cgaccgaaca
     4681 cgtcagaaga gagcgtaatc agcgcgccag acagtgcctg gtgagcaaaa ccaccgatac
     4741 acagcagcat aattgcgaca tacgggttgg tgaacaaacc gatcataccc gggccaatca
     4801 tcagcacagc acccagcgtt acgaccatct tacgggaaac gatcaggttc acaccaaacc
     4861 aacgctggaa cagcggcggc aggtaaccac cgaggataca accgaggtca gcaaacagca
     4921 tcggcatcca ggcgaacatc gcgatctctt tcaggttaaa gccgtaaact ttaaacatga
     4981 acagcgggat ccacgcgtta aaagtacccc aggccggttc tgccaggaaa cgcggcagcg
     5041 cgatacccca gaactgacgg ttacgcagga tctgaccaac ggacattttc ttcgccgtgc
     5101 tcacctggtg ctgggcttcc tgaccattaa taatatagtc gcgttcttca tcggtcagat
     5161 gcttctggtc gcgcggatgt ttatagaaaa tcagccatgc catcgcccag ataaagctca
     5221 atgcaccgga gatgataaat gccatctgcc agctgtgcat tacgattgcc cataccacca
     5281 gcggcggcgc aatcatcgca ccaatcgaag aacctacgtt aaagtaacct accgcgatgg
     5341 aacgctcttt cgccggaaac cattcggagc tggctttcag acccgccggg atcatcgctg
     5401 cttccgcggc accgaccgca ccacgagcaa cagccaggcc accccagcta cctgccagcg
     5461 cggttgcacc acagaacacg gcccacagca cagcaaacat tgcataaccg attttcgtac
     5521 ccagcacatc cagtacataa cctgctaccg gttgcatgac cgtataagca gcagaatagg
     5581 ctgcgatgat ataggaatac tgttgggtgg agatgtttaa ctcttccatc agagttggcg
     5641 cagctgccgc cacagtgtta cgcgtcaggt aaccaagcac ggtgccgagc gtcaccagtg
     5701 cgatcatata ccaacgtaac cctttaattt tacgcatgta aaacctcatc gttttgttat
     5761 atccgcagga agcgcggcca tcatcccgcc agaggctgaa gatgttagtt acgaccgggt
     5821 aaccgaaaaa aaaccggcac gccccgaacg ttgccatgaa agtttcgtgc taattcggct
     5881 tccgtaccgg ttaacccgat tcatctaaca aatgaatgaa tcggcaattt gcattccgtc
     5941 gacttatgat ttgcgacggc agaaagataa cttgtcatac aactttaaaa ggtgagagcc
     6001 atcacaaatg tgggaatatt tgtagggaca ttacctgacg acagcgaggc cagtactggc
     6061 gcggcctgca gcgagattta ccactttgag agtaattttt ttaactacgt ttattgatct
     6121 aactcacgaa aatatcttcg gactctggaa attggtgtga taactttgtc agcatcgcac
     6181 cataagcaag ctagctcact cgttgagagg aagacgaaaa tgactccgtt tatgactgaa
     6241 gatttcctgt tagataccga atttgcccgc cgtctgtatc acgactacgc aaaagaccag
     6301 ccgattttcg attaccattg ccatttgccg ccgcagcaga ttgcggaaga ctatcgtttt
     6361 aaaaacctgt atgacatctg gctgaaaggc gatcactaca aatggcgcgc tatgcgtacc
     6421 aacggtgtgg ccgagcgtct gtgtaccggt gatgcgtctg accgtgaaaa atttgacgcc
     6481 tgggcggcga ctgttccgca cactatcggc aacccgttat accactggac gcacctcgaa
     6541 ctgcgtcgtc cgtttggtat cactggcaaa ttgctttctc cgtcaactgc cgatgaaatc
     6601 tggaacgaat gtaacgaact gctggcgcag gataacttct ccgcgcgcgg catcatgcag
     6661 cagatgaacg tgaaaatggt cggcaccacc gatgacccga tcgattctct ggagcatcac
     6721 gcagagatcg ccaaagacgg ctctttcacc atcaaagtgc tgccgagctg gcgtccggac
     6781 aaagccttca acatcgaaca ggcgaccttt aacgactaca tggcgaagct gggcgaagtt
     6841 tccgataccg acattcgccg ctttgctgac ctgcaaactg ccttgaccaa acgtctggat
     6901 cacttcgccg ctcacggctg taaagtgtct gaccatgcgc tggacgtggt gatgtttgct
     6961 gaagcgaacg aagcggaact ggacagcatt ctggcgcgcc gtctggctgg cgaaaccctg
     7021 agcgagcacg aagtggcaca gttcaaaact gcggtactgg tgttccttgg tgccgaatat
     7081 gcacgtcgcg gctgggtaca gcagtaccac attggcgcgc tgcgtaataa caacctgcgt
     7141 cagttcaaac tgctggggcc ggatgtaggc tttgactcca tcaacgaccg tccgatggcg
     7201 gaagagttgt ctaagctgtt gagcaagcag aacgaagaaa acctgctgcc gaaaaccatt
     7261 ctctactgcc tgaacccgcg cgataacgaa gtgctgggca ccatgatcgg taacttccag
     7321 ggcgaaggta tgccgggcaa aatgcagttc ggttccggct ggtggtttaa cgatcagaaa
     7381 gacggtatgg aacgtcagat gacccaactg gcgcagctcg gtttgctgag ccgctttgtc
     7441 ggtatgctga ctgacagccg tagcttcctg tcatacaccc gtcacgaata cttccgccgc
     7501 attctgtgcc agatgatcgg tcgctgggtg gaagcgggcg aagcaccggc ggacatcaac
     7561 ctgctgggcg agatggtgaa aaatatttgc tttaacaatg cgcgtgacta cttcgccatt
     7621 gaactgaact aaggtctggg gttgatatgc aatacatcaa gatccatgcg ctggataacg
     7681 tcgcggtcgc tttagcagat ttggctgaag gcacagaagt cagtgtcgat aaccagactg
     7741 ttacgctgcg ccaggatgtt gctcgtggac ataaatttgc gttaacggat atcgcaaaag
     7801 gggccaacgt catcaaatat ggcctgctga ttggttatgc attggcggat attgcggcgg
     7861 gggaacacgt ccacgcccac aatacgcgca cgaatctgag cgatctggat cagtatcgct
     7921 atcaacctga ttttcaggat ctgcctgcgc aagcggcaga tcgtgaagtg cagatctatc
     7981 gtcgcgctaa cggcgatgtc ggggtgcgta atgagctgtg gatcctgcca accgtgggct
     8041 gtgtcaacgg catcgcgcgg caaatccaga accgtttcct gaaagagacc aacaacgccg
     8101 aaggtaccga cggcgtgttc ctcttcagcc acacctacgg ctgttcacaa ctgggcgacg
     8161 atcacatcaa cacccgcacc atgctgcaaa acatggtgcg ccacccgaac gcgggcgcag
     8221 tgctggtgat tggtctgggc tgtgaaaaca accaggttgc cgcattccgt gaaacgttgg
     8281 gcgatatcga tcctgaacgc gttcatttca tgatctgcca acagcaggat gatgagatcg
     8341 aagccgggat cgagcatttg catcagctgt ataacgtgat gcgcaacgat aaacgcgagc
     8401 caggcaaact cagcgaactg aagtttggtc tggagtgcgg tggttctgac ggtctttctg
     8461 gtattactgc taacccgatg ctggggcgtt tctctgacta cgtgattgct aacggcggta
     8521 ctaccgtact gaccgaagtg ccggagatgt ttggcgcaga gcagttgctg atggaccatt
     8581 gccgcgacga agcaacgttt gaaaaactgg tcaccatggt caacgacttt aaacagtact
     8641 ttattgccca tgatcagccg atctacgaaa acccatcacc ggggaacaaa gcgggcggta
     8701 tcaccacgct ggaagacaaa tcacttggct gtacccagaa agcgggttcc agtgtcgtgg
     8761 ttgacgtgct gcgttacggc gagcgtctga aaacgccagg gctgaacttg ttaagtgcgc
     8821 cgggtaacga tgccgtagcg accagcgccc tggcgggtgc gggctgccat atggtgctgt
     8881 tcagtactgg tcgcggcacg ccatacggtg gatttgtgcc gacggtgaaa atcgccacca
     8941 acagtgaact ggcggcgaag aaaaaacact ggatcgactt tgacgcgggt cagctgatcc
     9001 acggtaaagc gatgccgcag ttgctggaag aatttatcga caccatcgtt gagtttgcca
     9061 acggtaagca aacctgcaac gagcgtaacg acttccgcga actggcgatc ttcaaaagcg
     9121 gcgtaacgct ataaagtcga aatgagttgt taataaaaaa cggcgtttca taatgtgaag
     9181 cgccgtttgc ttttccatac ctgctaagga tttatcatga ccgcgtattg gctggcccag
     9241 ggcgtgggtg tcatcgcctt tctgattggt atcacaacat ttttcaatcg tgacgaacgt
     9301 cgcttcaaaa agcagctttc ggtctatagc gccgttattg gcgtacattt ttttcttctg
     9361 ggcacctatc ccgctggtgc cagtgccatc cttaatgcca ttcgtacatt gattacctta
     9421 cgcacgcgca gcttatgggt aatggcgatt tttattgtgc tgactggcgg aattggcctc
     9481 gcgaagttcc atcatcctgt cgaactattg ccggttatcg gtacgattgt cagtacctgg
     9541 gcactgttcc gctgtaaagg gctcaccatg cgctgcgtga tgtggttttc aacgtgttgc
     9601 tgggggattc acaacttctg ggcggggtcg ataggcggca cgatgattga gggtagtttt
     9661 ctgcttatga atggcctgaa tatcattcgt ttctggcgga tgcagaaaag gggaattgat
     9721 ccgtttaaag tagagaaaac ccctcccgcc atagacgaaa ggggttaaac aattaattac
     9781 gcagggcgct atttgccaga cgatcgtctt ccgcctggca agctgccgca gtgaacagca
     9841 cgtcagttga agagttcagc gcggtttcgc aagagtcctg caatacgccg ataataaagc
     9901 cgacggcaac cacctgcatg gcgatatcgt tcgaaatacc gaacatatta caggccagtg
     9961 ggatcagcag cagagaaccc cccgccacgc cggatgcgcc acaggcacac agagaagcca
    10021 ctacgctcaa cagcagcgct gtgggcagat cgaccggaat acccagcgta ttaaccgcag
    10081 ccagcgtcaa cacggtaatg gtgattgctg cgcccgccat attgatggtg gctcccagcg
    10141 gaatagaaac ggaataggta tcgcgatcca gattcagctt ttcacacagc gccatattca
    10201 ccggaatgtt cgctgcagag ctgcgggtga agaaggcata cacaccgctt tcgcgcaggc
    10261 acagcagcac cagcgggaac gggttacgac gaattttcca ccacaccagc aatgggttaa
    10321 ccaccagcgc caccagtaac atacagccaa ccagcacgac cagcagttgc gcgtagcccc
    10381 acagtgtgga gaaaccggtg gttgccaggg tagaagaaac cagcccaaaa ataccaatcg
    10441 gtgcgaagcg aatgaccagt ttcaccataa aggtaacggc attcgacata tcgttaacca
    10501 ggtttttggt ggtctcgtta ccgtgacgca gtgcgaagcc gaggccgatc gcccacacca
    10561 gaatcccgat gtagttacct ttcagcagcg cgtcgatggg gttggaaacc atgctcatta
    10621 ccagcccgcg catcacttcg acaatgcctg acggcggcga aatatcaccc gcgctactgg
    10681 ataaatgcag ggtagaaggg aaggcaaagc tgaagactac tgcggccaga gcagcagaga
    10741 aggtgcccag tagatagagg aacaaaatag ggcggatatt ggttttctgc ccgtgctggt
    10801 ggttagcaat agatgccatt accagcatca acaccaggat gggggcaacg gctttcagtg
    10861 cgccgacgaa caaagtacct aacagaccaa cagcttccgc cgcgggtttt gagatccatg
    10921 ccagaagaat ccccagaaca aggccgacca ggatttgttt taccaggctg ccatgagcca
    10981 gacgccggaa tagccccggt gaacgttgcg tagtcatttt tcgatccttt cattgtgttg
    11041 tcgcacatcc ctggtgcgtt ctgtcgacgc atctttcagg atgtaacaaa gtgtttgcaa
    11101 ggtcgagtat aaggaaagtt acagcttcgg gaagcgaaaa atgctggatt ttaaagagag
    11161 gtcatatttt gcaacttaat ggttacattt atttgacata aaaataaagc caacgtgcgg
    11221 cttccagact gatgacaaca tcaaaaaagg actcggtcag tcccctcgcc ccgtttgggg
    11281 agagggttag ggtgagggga acaggccagc atcggtgcga aaaattaccc tcaccccgac
    11341 cctctccctg gaagggagag ggagaaaacg gtgctcatgt taatgacagg gtacacggct
    11401 taggcagatt aaaaattatc caccccgctg cttatcatgc cgataattca cccaggcgtt
    11461 gataataaac gtcatcacca gaatgccaaa caccacgccc agcgagacgg cgattggaat
    11521 atggtagaag tcgacaatca gcatcttgat accgataaac accaggatca ccgccaggcc
    11581 atatttgagc atcgagaaac gctctgctac gcccgccagc aggaaataca tcgcacgcag
    11641 gccgaggatc gcaaacaggt ttgaggtcag cacaatgaac gggtcagtgg tcacggcgaa
    11701 gatagccgga atgctatcca cggcgaaaat cacgtctctc aattccacca gaatcagtac
    11761 cagcatcagc ggtgtggcgt acagcaatcc attcttacgc acaaagaaat gctcgttgtc
    11821 gatggtgtcg gtcatgcgca aatgaccgcg tagccagcgc accagcggct tgtcgccaat
    11881 gcctgattca tcttcatggg tgagcgccat cttcacgccg gtaaacagca ggaaggcacc
    11941 gaagatatac aggatccagt cgaactgcga aattagccag ctgccagtga agatcatgat
    12001 ggtacgcaga acaatcgccc cgagcacgcc atacaccagc acgcggcgtt gtaatgccgc
    12061 cggaacagag aaatagctga acaacatcag ccagacaaag acgttatcga ccgccagcga
    12121 tttctcaatc agataacctg tgagaaaggc cagtgcctgt ggatcggcaa cggcgcgacc
    12181 ttcggtttgc accagatacc accagaaagc ggcgttaaac agtaacgaca gcgtcaccca
    12241 gaccagcgac caggccgcag cctgtttcat ggtcatggca tgtgccccac gacgcccctg
    12301 caacaacagg tcgatagcca gcataatggc gacaacaaca gcgaatccgc cccatagcaa
    12361 cggcgtgccg acagtattca tagaagttcc ttacacataa aaaaacggcc aacgtcggaa
    12421 gacgtcagcc gctgcttttt atgcatagac ctcgccttcc ggcaaggtct cacttacaac
    12481 aaaaaaggaa tacgtctgcg tattcctttc gggttgcccg gtgaccggat gtggtttttc
    12541 acacatcgta atgacgatcg accggcaatg aagttactcc cctttgcagg taacaaagta
    12601 tgacagacca ttcagccagt caatggtctg ttacatacct tttacactgt tttactttgg
    12661 caattatagt tttacgctat ctgccggaaa aatcacccca gtctggcggc ggatctcggt
    12721 cagcagtttg gcggtgatgc gactgaccgc cagccccgga tgatccacca gatgctcatc
    12781 caccagggta gcgaacagct ctgcttcgta gagcatggta ttaatatgct gcggctgggt
    12841 gagatcctgc atttggctgc cacgcggcac gaagcacact ttctggcatt cagacagttt
    12901 ttcaatcacc agcgatcctg cttcgccctg aatctcgctc gccaggacag aatcactgac
    12961 ttttgagtgc tgcaaggtga cgctgaaatc accgtaatcc atcaccacca cgccgtgggc
    13021 atctacaccg cttgccagca aactggcggt tgcctgcacg cttttcggct caccaaataa
    13081 cgccaccgcc gacgccaggc agtaaaagcc gatatccata attgaaccgt tagagaatga
    13141 cggattaaag gtgttgggat tttcaccgtc caggtaacgt tgatagcgcg aggaatactg
    13201 gcaatagttg aaaaagactt tacgcaattt gccgactttc ggcagcgcct ggcgcagcaa
    13261 atgaaagttc ggcaggcagg cggttttaaa tgcctcaaac agcaccacct gattttcccg
    13321 cgcacaggca atggcggcat ccacttccgc cagattcgac gccagtggtt tctcgcaaat
    13381 cacatgaatt ttatggctaa ggaaaagttg tgtctgggaa aagtgcaggg cattcgggct
    13441 ggcaatatac accgcgtcaa tggcatcgct ttccgccatc gcttccagcg aggtaaacag
    13501 atgctcgaca gaaaaatcat tggcgaagtg ctgagcctgc tcaaggctgc gggaatatac
    13561 ggcggttaac ttgtatttac cgctctcatg ggcggcctcg acgaactggc gagtgatcca
    13621 gttcgtacca atcacagcga aacgtatcat aacgcgtgga tactccataa agggattctg
    13681 tcgtcaatgt agcacgcctt tttcatcttc gacgtgcgca gatacgcatt attcttttgc
    13741 cggacagcca tgctcactaa acggcccaat catgaccgat ggcccgagat atttcggctg
    13801 gcgatggagg attttggcgt caatcacggc gctgacacga gcgagtttct cccgcagttg
    13861 agtataaaaa ccgtgcttca tattgaggtc aggcgcgtta aaaccgatag cgtcgatgct
    13921 gtactgtttt gccagccaga tggcgcgttg gttatgaaat tcttgcgaga taatggtgat
    13981 atggttttca ccaaaaacct ttttggcacg cactaccgaa tccagcgttg agaatccggc
    14041 atagtcgcag aagatcactt tcgccggtac gcctttagcg atcaaagcct tctgcatacc
    14101 tgatgcttca tcgtaatttt tacgtccgtt atcgccactt accagtagcc atttcacttt
    14161 tccggcgtgg tacaacgctg ctgcggtatc aatacgtcgg gtaaagtagc gattacccgg
    14221 cctggccccc agcaataagc ccacgttacg ctccggaact gcgttgacat cgctccaggt
    14281 cagctgcttg ctggcgttga ccataacgcg atcggcaata aaaatggtcg ctcctgctat
    14341 aagcaacaac aaacagccaa tcttaagagt ccggcgagaa aaacaaatac ggagaagaag
    14401 gcggggctgc atggtcgtta tccttaacga aaggggggag gtcgatttta ggcgctaaag
    14461 aaagaaaaga taacgcctga catcattttc agattttgtc ctgcccaagc gacaactgcg
    14521 ttagccacag ccgggtgtcg aactcaagct ggtggtactg cggctccata tggcagcaca
    14581 attgatagaa cgctttgtta tgctctttct ccttcaggtg cgccagctcg tgcaccacga
    14641 tcatgcgcaa aaacggttcc ggcgcgttgc gaaacacggt agcgacgcgg atctccgctt
    14701 ttgctttcag cttgccgccc tgcacacgag aaacagcggt atgtaacccg agcgcgtttt
    14761 ttagcacatg gatcttattg tcatacatca ctttattgat cggcggggca ttgcgcagaa
    14821 actgattttt tagatcctga gtatattgcc agagggcttt atcggtggcg tagtcgtgtg
    14881 ttcccggata gcgttttgcc agcacatcgc ccagacgctg ttcgttaatc agcgttcgca
    14941 cctgggaaag tagctgctcg ggataaccct gaagataagt aagattgctc ataatcgccc
    15001 acgaaaaaga gaaaaagggt atactcacgc acccttttca ggggcaacgt cgaaatttta
    15061 tcattcagga gggccgatga gccacttaga caacggtttc cgttcactga cactacaacg
    15121 ttttccggcg acggatgacg ttaacccgct acaggcgtgg gaagcggcgg atgaatattt
    15181 gctgcaacag ttggacgaca cagaaatccg cggcccggtg ttgatcctga atgatgcctt
    15241 tggtgcgtta agctgcgcgc tggcggaaca taagccgtac agcattggcg actcatacat
    15301 cagtgaactg gcgacgcgcg agaatttacg cctcaacggg attgatgagt cgagcgtgaa
    15361 gtttctcgac agcaccgccg actacccgca acagccgggt gtggtgctga tcaaagtgcc
    15421 gaaaacactg gcattgctgg aacaacaact gcgtgcgctg cgcaaagtgg tcacgtcgga
    15481 tacacgtatt attgccggtg ccaaagcccg tgacattcac acttccacgc tggaactgtt
    15541 cgaaaaagtg ctcggcccga ccaccaccac gctggcatgg aaaaaagcgc gcctgattaa
    15601 ttgcacgttc aatgaaccgc cgctggccga tgcgccgcag accgttagct ggaagctgga
    15661 aggtactgac tggactatcc ataaccatgc gaatgtcttc tcccgcaccg ggcttgatat
    15721 cggcgcgcgc ttctttatgc aacatctgcc agagaatctc gaaggggaga ttgtcgatct
    15781 cggttgcggt aatggcgtta ttggtctgac gctgcttgat aaaaacccgc aggcgaaagt
    15841 ggtgtttgtc gatgaatcgc cgatggcggt tgcttccagc cgtttgaacg ttgaaaccaa
    15901 catgccagag gcgttggatc gcagcgagtt tatgatcaac aacgcgctct ccggcgtgga
    15961 gcctttccgc tttaatgctg tgctctgcaa cccgccgttt caccaacaac atgcgctgac
    16021 cgataacgtc gcctgggaga tgttccacca tgcccgccgc tgcctggaaa tcaacggcga
    16081 gctgtatatt gttgccaacc gtcacctgga ttacttccat aaactgaaga agattttcgg
    16141 caactgcacc accatcgcca cgaataataa atttgtggtg ctgaaagcag tgaagctggg
    16201 gcgtcgtcgg taagattggg agttacctgc cggatgcggc gttaacgcct tatccggcct
    16261 acgttcagca cgttactccc aggccggata agacgcggca agcgtcgcat ccggcaatgt
    16321 ctgcccagtt ctggaatcgc gcttccataa aacaaaaaat ccctctcacc atcctctaca
    16381 ttctcttgtt tagcgttttt ctacgtttat tcttccgtca cacagataaa ttcatccgtt
    16441 gccaatctgt caacggatgt tatcatattg ccaatttgtc aacggacgtg atgaagaatg
    16501 cacctgataa ctcaaaaagc attgaaagat gctgcggaaa aatacccgca acataaaacg
    16561 gagttggtgg ctctggggaa cacgattgct aagggatatt tcaaaaaacc tgagtcatta
    16621 aaagcagtat tcccatctct ggataacttc aaatatctgg ataagcatta tgttttcaat
    16681 gttgggggca atgaattacg tgttgtagca atggtctttt ttgaatcgca aaagtgctac
    16741 atacgtgaag ttatgacgca taaagaatac gatttcttta ccgctgttca tcgtactaag
    16801 gggaaaaaat gattgcgatt gccgacatct tgcaagcagg agaaaagcta actgctgtgg
    16861 caccttttct ggcgggtatt cagaacgagg aacaatacac ccaggcgctg gaactggtag
    16921 atcatctgct gctcaacgat cctgaaaacc ccttgctgga tctggtgtgt gcaaaaataa
    16981 ccgcgtggga agaatcagcg cccgaatttg cggaatttaa tgccatggct caagccatgc
    17041 ctggcggtat agccgtgatt cgtaccctta tggatcaata tggtttaacc ctttccgatc
    17101 tgccggaaat tggcagtaaa tctatggtgt cacgcgtttt gagcgggaag aggaaattaa
    17161 cgctggaaca cgctaaaaaa ttggcaacgc gattcggcat ttctcccgcc ttg
//
LOCUS       DAZKIV010000100        17099 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZKIV010000100 DAZKIV010000000
VERSION     DAZKIV010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.100"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..1224
                     /gene="arnB"
                     /locus_tag="ACHOT6_003176"
     CDS             85..1224
                     /gene="arnB"
                     /locus_tag="ACHOT6_003176"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFP9948346.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1228..2196
                     /gene="arnC"
                     /locus_tag="ACHOT6_003177"
     CDS             1228..2196
                     /gene="arnC"
                     /locus_tag="ACHOT6_003177"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFP9948347.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFAHRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2196..4178
                     /gene="arnA"
                     /locus_tag="ACHOT6_003178"
     CDS             2196..4178
                     /gene="arnA"
                     /locus_tag="ACHOT6_003178"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFP9948348.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4175..5065
                     /gene="arnD"
                     /locus_tag="ACHOT6_003179"
     CDS             4175..5065
                     /gene="arnD"
                     /locus_tag="ACHOT6_003179"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFP9948349.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQANSGNWDRQTLVDDIARGLHTLEEIIGQPVTCSAAAGWRADQQVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSGTLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5065..6717
                     /gene="arnT"
                     /locus_tag="ACHOT6_003180"
     CDS             5065..6717
                     /gene="arnT"
                     /locus_tag="ACHOT6_003180"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFP9948350.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLTMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6714..7049
                     /gene="arnE"
                     /locus_tag="ACHOT6_003181"
     CDS             6714..7049
                     /gene="arnE"
                     /locus_tag="ACHOT6_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFP9948351.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7049..7435
                     /gene="arnF"
                     /locus_tag="ACHOT6_003182"
     CDS             7049..7435
                     /gene="arnF"
                     /locus_tag="ACHOT6_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFP9948352.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(7429..7695)
                     /gene="pmrD"
                     /locus_tag="ACHOT6_003183"
     CDS             complement(7429..7695)
                     /gene="pmrD"
                     /locus_tag="ACHOT6_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFP9948353.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(7805..9160)
                     /gene="menE"
                     /locus_tag="ACHOT6_003184"
     CDS             complement(7805..9160)
                     /gene="menE"
                     /locus_tag="ACHOT6_003184"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFP9948354.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGAEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9157..10119)
                     /gene="menC"
                     /locus_tag="ACHOT6_003185"
     CDS             complement(9157..10119)
                     /gene="menC"
                     /locus_tag="ACHOT6_003185"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFP9948355.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMTSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYESVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10119..10976)
                     /gene="menB"
                     /locus_tag="ACHOT6_003186"
     CDS             complement(10119..10976)
                     /gene="menB"
                     /locus_tag="ACHOT6_003186"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFP9948356.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10991..11749)
                     /gene="menH"
                     /locus_tag="ACHOT6_003187"
     CDS             complement(10991..11749)
                     /gene="menH"
                     /locus_tag="ACHOT6_003187"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFP9948357.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLTG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVVLRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11746..13416)
                     /gene="menD"
                     /locus_tag="ACHOT6_003188"
     CDS             complement(11746..13416)
                     /gene="menD"
                     /locus_tag="ACHOT6_003188"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFP9948358.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13505..14800)
                     /gene="menF"
                     /locus_tag="ACHOT6_003189"
     CDS             complement(13505..14800)
                     /gene="menF"
                     /locus_tag="ACHOT6_003189"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFP9948359.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="ACHOT6_003190"
     CDS             complement(14879..15184)
                     /gene="elaB"
                     /locus_tag="ACHOT6_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFP9948360.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15239..15700)
                     /locus_tag="ACHOT6_003191"
     CDS             complement(15239..15700)
                     /locus_tag="ACHOT6_003191"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9948361.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15765..16682
                     /gene="rbn"
                     /locus_tag="ACHOT6_003192"
     CDS             15765..16682
                     /gene="rbn"
                     /locus_tag="ACHOT6_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFP9948362.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16870..>17099
                     /locus_tag="ACHOT6_003193"
     CDS             16870..>17099
                     /locus_tag="ACHOT6_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HFP9948363.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPT"
BASE COUNT         3983 a         4626 c         4351 g         4139 t
ORIGIN      
        1 atttcgctcc aaatggcaaa atatacacaa cactctttat agcaaatata agtggacagg
       61 tattcaatgg cggaaggaaa agcaatgtca gaatttttgc ctttttcgcg accagcaatg
      121 ggcgtggagg aactcgctgc agttaaagag gttctcgaat ccggttggat cacaaccggt
      181 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
      241 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      301 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
      361 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      421 gcgatcgagt cagccataac gccacgcact aaagccatca ttccggtgca ttatgccggt
      481 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      541 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      601 accgctattt tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt
      661 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggtctgggt
      721 gtcgatgcct atgacagaca aacctggggc cgtgcaccgc aggctgaagt cttaacaccg
      781 ggctataagt acaatctgac cgatattaac gccgcgattg ccctgacaca gttagcaaaa
      841 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
      901 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      961 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
     1021 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
     1081 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1141 tgttcgttgc cgctgttccc ggatatgact accgccgatg cggaccgcgt catcacagcc
     1201 cttcagcaac tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1261 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     1321 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     1381 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     1441 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     1501 actggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
     1561 cgcctggtgg caaaagccga tgaaggttac gacgtggttg ggactgtacg ccagaaccgc
     1621 caggacagct ggtttcgtaa aaccgcttcg aagatgatta accggcttat tcagcgcacc
     1681 actggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
     1741 gatgcgatgt tgcactgcca tgaacgcagc acctttatcc cgattctggc gaatatcttc
     1801 gcccaccgtg caattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     1861 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1921 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     1981 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     2041 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc
     2101 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2161 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2221 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     2281 tttttaccca taccgataat cccggtgaaa aagcctttta tggttcggtg gctcatctgg
     2341 cggcggaaag aggcattccg gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2401 aacgcattgc ccaactgtca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2461 gcgacgagat tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     2521 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acagaaactg
     2581 gcgttacatt gcaccgaatg gtaaaacgtg ccgatgccgg ggccattgtg gcgcaactgc
     2641 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg
     2701 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
     2761 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttcctcg
     2821 aatggcataa gccggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     2881 cgggtgcttt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2941 ctcatgccag caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct
     3001 gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg
     3061 gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     3121 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtaaatggc tttattggca
     3181 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     3241 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtc gaaggcgata
     3301 tcagtattca ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc
     3361 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3421 attttgaaga gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct
     3481 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     3541 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat
     3601 tacttgatcg ggttatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     3661 gcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct
     3721 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg
     3781 atggcggaaa acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc
     3841 gcattatcga aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg
     3901 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     3961 cgctgcgcca tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact
     4021 acggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccgccgct
     4081 gcctggactg ggaaccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     4141 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtcggct tacgcattga
     4201 tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa
     4261 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4321 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4381 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4441 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     4501 tcaccatgcc tggcaagcca atagcggtaa ctgggatcgg caaacattgg tcgacgatat
     4561 tgcacgcggc cttcacaccc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4621 tgcgggctgg cgtgccgacc agcaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4681 caacagcgat tgtcgtgggg ccatgccgtt ccgtccactg ctcgaatcag gaaaccctgg
     4741 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     4801 agcagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     4861 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4921 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagggac
     4981 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     5041 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg
     5101 tttattgcct gctattacct gttaccgatc agcacacgtc tgctctggca accagatgaa
     5161 acgcgttatg cggaaatcag tcgggaaatg ctggcatccg gcgactggat tgtgccccat
     5221 ctgttagggc tacgttattt tgaaaaaccc attgccggat actggattaa cagcattggg
     5281 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5341 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5401 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5461 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctgaca
     5521 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5581 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5641 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5701 atcagttgcg tactgacggt tctcccttgg ggactggcga tagcgcagcg ggagcctgac
     5761 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     5821 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     5881 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     5941 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg
     6001 cccacctata ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg
     6061 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     6121 ggcgtcactg gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg
     6181 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     6241 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggtcttt tgccgcgctt
     6301 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     6361 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt
     6421 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6481 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     6541 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6601 attacactcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     6661 gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     6721 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6781 caacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6841 cgctggcttg tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     6901 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     6961 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg
     7021 gcattgtgat cctcgggagt acggtgtaat gggcctgata tggggattat tcagcgtcat
     7081 tattgcctca gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac
     7141 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     7201 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc ttcataaact
     7261 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7321 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7381 tattatgagc gggttgatgc tgattttcct gcccatgaca aaacaacgtt actgagtttt
     7441 ccctgccact ttacactgcc gctcccactc atcggggcta taactgcttg ccgagaggac
     7501 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     7561 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7621 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     7681 gaccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     7741 aacagataaa ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg
     7801 ttaattattg ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc
     7861 cgtttttcag ttcaggcggc aacgttaacc aacgcaccgg ttgctgaaaa cgggccagct
     7921 tatctttcac ccattcacta agatcaacgc tctcgtggtc atactccatc accgccaccg
     7981 gtcgatgacc aaactccttg tcggcaacgg ggacgataaa cacctgcaaa accgcaggat
     8041 gtgcagcaat tacgcgctcg acttcttccg gctgaatacc ctctgcgcca ctgaagaata
     8101 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8161 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     8221 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     8281 gcagcggcga accaacgtct gccaggccgt cggcttcttt cgcacacacc gtggaggcaa
     8341 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     8401 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt
     8461 taaccagcaa acgccagagt tgtgttggca ccagtgaagc atgagtacag cctgccagca
     8521 tttgctccaa tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc
     8581 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
     8641 cgccaaacgg aatcaacgat aacacccctt cggcactggc aagatgggcc tgataagtat
     8701 ggacagcggc tttcggcaac ccggtagaac ccgaggtcaa cgtcattgag cacagacgcg
     8761 tcggctgcca cgtagcggca tgtgcgcctt caacccgctg aatgtgcagc gacgttaacg
     8821 ccggaaacgt gttttcccca tccggcacca gcgcaaattg cagcgtcaga ttgggcagca
     8881 attcttcaag caacggttgc ggcagctgag ggttcacggg caacacccgc gccccgcatt
     8941 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     9001 cgctgccctc aacaacgccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac
     9061 aaagctcgcg ccagttaagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9121 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc
     9181 aacttccacg acaggaagcg tgctacccgg ccagcgacgt acctgctgcg cctgcatcag
     9241 atccagcgtg tccagccctg gaatggtgtc cggcgttaac caggcggcaa tccgcgccag
     9301 ttgcgttaag cctaagctcg attcaatgga agaactgatc accgccgtca gccccagcgc
     9361 gtgcgccgcc tgtacctgct cgcgtacttt ctccagactg cccgtgagcg tgggtttgat
     9421 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaagctttc
     9481 atcccaggca atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt
     9541 gcacggctct tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa
     9601 ctgctggcct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
     9661 aattgcctcc agcaacagat taaccaccat gccgtcgcgc acggattcgt acaatcccac
     9721 tttgaccttc gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg
     9781 atcgccatta cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
     9841 ctccgccaat gcacagctta cgccaaaggc aacggaagtc atctgcggta gctcgcaatc
     9901 gcctgccagc cagttattta cccaggcaag cagcacgctt tgcgcctctt cccaggtttc
     9961 ctggctgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag
    10021 gcaaacgtac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc
    10081 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10141 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    10201 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag
    10261 tcggcgttca gcgcagcttt caggcagcgc agcgccatcg ggctgttttg cagcatttcg
    10321 cggcaccaac ggacggtttc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    10381 cccatatcca gcgcctgttt tgcgtcgtac tgacggcaca ggaaccagat ttctcgcgct
    10441 tttttctgcc cgacgatacg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10501 actttcgggc cagtctgacc gaagatggca ttatctgctg cgatagtcag gtcgcacatc
    10561 atgtgcagaa cgtgaccacc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10621 ggacaggtac ggatctgacg ctggaagtcc agcacgttca agtgatgtac gccggaatca
    10681 tctttatagc cgccgtaatc accacgcact ttctggtcac caccggagca gaacgcttta
    10741 tcgcctgcgc cagtcagaat gatcacgccg atattgtcgt cataacgcgc atctgccagt
    10801 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10861 atggtgattt ttgcgatacc gtcggtggat ttttcataac gaatgtcctc gaaaccttcg
    10921 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    10981 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt
    11041 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    11101 ccagggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11161 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    11221 caagggttgc gccattattg ttgctgcgca gcaccaccag ctcccggcgt tgatcgtcat
    11281 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    11341 tgcaaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat
    11401 tttgcagccc cggatgcccg ccttcgacaa taaccccaca aagccccgtc agcccctggc
    11461 aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga
    11521 tgttgtaact aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    11581 taatcgccgc cgaaccacca tgacctggga gatcaacata caaccgtgag tagtcggcaa
    11641 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    11701 gccagggtaa acctggtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11761 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11821 caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt
    11881 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11941 cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg
    12001 cccgccgttg ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc
    12061 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12121 gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg
    12181 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    12241 caggctgtta ccaacaaaca attgcccctg ttcaggcaga tagtcgcaga tgcgatgcgc
    12301 cagttgcgct tcgccaaacg catcacggcg ggcaataacc gcctgcattg cctgttccgc
    12361 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12421 gtcggcaata ttggcaatta agcgacgtcc gcgatggtgt gccggatcaa gtcgcccttc
    12481 aatgtcatca acaatccagt actcttctgg ttcacagctt gcctgccatt gcaggagccg
    12541 tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt
    12601 cgctttggca ttgcctaacc aaagatcggc acacggcagc ggctgcccgg tttgtgacag
    12661 cacatcgcca atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc
    12721 ttccgcactc atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc
    12781 gcgctgtttt tcactttcca gacgaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12841 ccagtcaccc agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg
    12901 ttcagcaaac gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc
    12961 gatggtagaa accagccaac gtgcggggat atcccgggtc gggcgcggca atgaaatact
    13021 gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc
    13081 aattagctcc ggcgggcgat cggcggttaa caggatcagt ttttctccgg ttaacccagc
    13141 ttcaatcagt gccggataga gatttgctac cgccgtgccg gaggtcacaa tcaccgccac
    13201 cggctgcttg ctgactttcg ccagccccag cgccagatgt cccagtccac gctcatcgaa
    13261 atgggtgtgg tgaatgaatg tggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13321 cgagccaggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13381 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13441 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13501 attactattc catctgtaat aaagtacgca gccctgccgc tttgttgtcg atttcctgcc
    13561 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    13621 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat
    13681 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    13741 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    13801 tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    13861 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt
    13921 ggcagatatc ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc
    13981 actctcctaa ctgctgcgcc tgcttatcat caggattatt tgctactgtt cccgccagcg
    14041 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    14101 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    14161 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    14221 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    14281 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    14341 gcaagggctt gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct
    14401 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    14461 attccaggcg gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa
    14521 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14581 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14641 agaattgcgg gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14701 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14761 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14821 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14881 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
    14941 gccagggttt ttcgtggacg tagtcatcag cacgataaac tgcctgcttc gcccgatagt
    15001 aataactgtc tgaagcctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    15061 gcgctttcag ctcaacatat ttctgatcgg cgggatcgcc agaggagcgg agcacctctt
    15121 ccagtgtttc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    15181 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
    15241 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    15301 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    15361 ggtaaacagg cttatcaggc cagtgatgcg tacatgtttc gagtgtttta ctcatcagct
    15421 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacct ataacgaccg
    15481 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
    15541 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    15601 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    15661 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    15721 atgccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    15781 taggtacttc ggccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    15841 tgcaacatcc gacccagagc ggtctttggt tgtttgactg cggtgaaggc acccagcatc
    15901 agctactgca taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc
    15961 atggcgatca tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    16021 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    16081 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    16141 ttctcgatga tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt
    16201 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    16261 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    16321 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    16381 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    16441 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    16501 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggtaagc
    16561 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg
    16621 aatgcaggtc aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacgttt
    16681 aactctgctt attattaaaa acagggcgaa acttgccctg ttatcgcaac ccgcgctaca
    16741 tatatccacc cacacgggca tattgagcga tttttgatat ttattcatct ccttacgcac
    16801 tatattcaga gcaataagtt gcctcttttt atcttcattc agcacaacct ttgcaactaa
    16861 aaggagccca tgatggttac agttgtcagc aattattgtc aattatctca aacccaactc
    16921 agtcagacat ttgcagaaaa atttactgtg accgaggaat tactgcagtc tttaaaaaaa
    16981 acagcgttat ccggagatga agaaagtatt gagttactgc ataatattgc gttaggttat
    17041 gatgaatttg gaaaaaaagc tgaagatatt ctttaccata ttgttagaaa cccaacaaa
//
LOCUS       DAZKIV010000101        16824 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZKIV010000101 DAZKIV010000000
VERSION     DAZKIV010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.101"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..852
                     /locus_tag="ACHOT6_003194"
     CDS             <1..852
                     /locus_tag="ACHOT6_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9948364.1"
                     /translation="VDSILSKVKHNNFGKLSQEIKDAFQLVNYNGSAFRSAKISDIYL
                     QARLNELKWGVAVNEIMLEEKEEQRRIKEQLREEERARREYEKAIKEAEKEEKAIQQA
                     INKATKELMLANEEQRLALEQKIAELQLKYEEAEAKNQRAISMAQQTRSGHVYIISNI
                     GSFGEDVYKIGMTRRLEPLDRVRELGDASVPFSFDVHAMIYSDDAPSLENHLHKVFNE
                     KQVNKINSRKEFFNVNIKEIKSVIEDMNINAHWTMFAEAKEYRESLAIEQERKAATSA
                     NDELHVA"
     gene            936..1988
                     /locus_tag="ACHOT6_003195"
     CDS             936..1988
                     /locus_tag="ACHOT6_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9948365.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVVELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAINLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLPSGDKVAAAVGITP"
     gene            complement(2093..2629)
                     /gene="rcdA"
                     /locus_tag="ACHOT6_003196"
     CDS             complement(2093..2629)
                     /gene="rcdA"
                     /locus_tag="ACHOT6_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFP9948366.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            2713..3921
                     /locus_tag="ACHOT6_003197"
     CDS             2713..3921
                     /locus_tag="ACHOT6_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9948367.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHISLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            3921..4736
                     /gene="ybjI"
                     /locus_tag="ACHOT6_003198"
     CDS             3921..4736
                     /gene="ybjI"
                     /locus_tag="ACHOT6_003198"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFP9948368.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            4822..5106
                     /gene="ybjH"
                     /locus_tag="ACHOT6_003199"
     CDS             4822..5106
                     /gene="ybjH"
                     /locus_tag="ACHOT6_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFP9948369.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(5147..6379)
                     /gene="mdfA"
                     /locus_tag="ACHOT6_003200"
     CDS             complement(5147..6379)
                     /gene="mdfA"
                     /locus_tag="ACHOT6_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFP9948370.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGQFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            6664..7260
                     /gene="ybjG"
                     /locus_tag="ACHOT6_003201"
     CDS             6664..7260
                     /gene="ybjG"
                     /locus_tag="ACHOT6_003201"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFP9948371.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            7318..8076
                     /gene="deoR"
                     /locus_tag="ACHOT6_003202"
     CDS             7318..8076
                     /gene="deoR"
                     /locus_tag="ACHOT6_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFP9948372.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(8123..9325)
                     /gene="dacC"
                     /locus_tag="ACHOT6_003203"
     CDS             complement(8123..9325)
                     /gene="dacC"
                     /locus_tag="ACHOT6_003203"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFP9948373.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTIFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            9572..10198
                     /gene="gstB"
                     /locus_tag="ACHOT6_003204"
     CDS             9572..10198
                     /gene="gstB"
                     /locus_tag="ACHOT6_003204"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFP9948374.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(10195..11310)
                     /gene="yliI"
                     /locus_tag="ACHOT6_003205"
     CDS             complement(10195..11310)
                     /gene="yliI"
                     /locus_tag="ACHOT6_003205"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFP9948375.1"
                     /translation="MHRQFFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(11421..11804)
                     /gene="bssR"
                     /locus_tag="ACHOT6_003206"
     CDS             complement(11421..11804)
                     /gene="bssR"
                     /locus_tag="ACHOT6_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFP9948376.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            12017..13342
                     /gene="rimO"
                     /locus_tag="ACHOT6_003207"
     CDS             12017..13342
                     /gene="rimO"
                     /locus_tag="ACHOT6_003207"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFP9948377.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(13389..14717)
                     /locus_tag="ACHOT6_003208"
     CDS             complement(13389..14717)
                     /locus_tag="ACHOT6_003208"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFP9948378.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(14725..>16824)
                     /locus_tag="ACHOT6_003209"
     CDS             complement(14725..>16824)
                     /locus_tag="ACHOT6_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9948379.1"
                     /translation="AVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVN
                     GPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKD
                     HDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLIIYNSEG
                     NYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFIIIVLLVV
                     YFMTTRVFRRQFSEMTELVNSLAFLPDSTDQIEALKIREGDAKEIISIKNSIAEMKDA
                     EIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVFGVDERN
                     KIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSSFGYRNL
                     RIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEENIWIARN
                     IRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAEDIGFIN
                     ELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMKENGLKA
                     NSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEINPDSIKV
                     DSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQAQGYLYQ
                     KPMPFSAWDKSGKLVKE"
BASE COUNT         4440 a         4153 c         3910 g         4321 t
ORIGIN      
        1 gttgacagta ttttatcaaa agtcaaacac aacaattttg gaaaactttc ccaagaaata
       61 aaagatgcat ttcaacttgt aaattataat ggctctgctt ttagatccgc aaaaataagt
      121 gacatctatc ttcaggcacg actcaacgag ctaaaatggg gagttgcagt taatgaaatt
      181 atgctcgaag aaaaagagga gcaaagaagg attaaagaac agcttcgtga agaggaaaga
      241 gctcgtagag agtatgaaaa agcgataaaa gaagccgaaa aagaagaaaa agctattcag
      301 caagctataa ataaagcaac gaaagagctt atgcttgcaa atgaagaaca acgcttagct
      361 ttagagcaaa aaatagctga actacagtta aaatatgaag aagctgaagc taaaaaccaa
      421 cgagctattt ctatggctca acaaactaga tcaggccatg tttatataat tagcaatatt
      481 ggctcatttg gcgaagatgt atataaaatt ggaatgacac gccgccttga accacttgat
      541 cgtgttcgtg aacttgggga tgctagtgtt cctttttcgt tcgatgttca tgcgatgatt
      601 tatagtgatg atgcaccgtc attagaaaat catctgcata aagtcttcaa cgaaaagcag
      661 gtcaataaaa ttaactcacg aaaagagttt tttaacgtaa atattaaaga aattaaatcg
      721 gttattgaag atatgaacat caatgcccac tggacaatgt ttgcagaggc gaaagaatat
      781 agagagtcac tagctattga gcaggaacgc aaagcagcca cttccgccaa cgatgaacta
      841 catgttgctt agcaatgtat gtttcatagc aatcacacat tgattactgg ttgcatatac
      901 agttaaattt agccctctga tatgagggct tttttatggc agtacgaaaa ctcaccacag
      961 gaaaatggct ttgcgaatgt taccccgccg gacgtagtgg acgtcgtgtg cgtaaacaat
     1021 tcgccaccaa aggcgaagca ctggcttttg agcgtcacac gatggaagaa accgaagcaa
     1081 agccctggct aggtgaatca gtggatcgtc gaacactgaa agacgtggtt gagctatggt
     1141 tcaaactaca tggtaaatca ctgacagctg ggcagcatgt ctatgacaaa ttgctgctga
     1201 tggttgacgc tctgggcaat ccccttgcaa ctgatctaac ctctaaaatg tttgcccact
     1261 atcgagataa acgcctgaca ggtgagatct acttcagcga gaaatggaag aaaggagcaa
     1321 gcccggtcac cattaacctg gagcaaagct atctaagtag tgtttttagc gaactatccc
     1381 gcctgggcga atggtcgtat ccgaacccac tggagaacat gcgaaaattc accatcgcag
     1441 aaaaagagat ggcatggctt acccatgagc agattgttga attactggct gattgcaaac
     1501 gtcaggaccc aattctggca ctggtagtta agatatgctt aagcacaggc gcacgctggc
     1561 gagaagccat aaatcttact cgttcacagg tgaccaaata ccgaattacc tttgtaagaa
     1621 cgaaggggaa gaaaaataga agcatcccta tcagtaaaga gctttacgaa gagatcatgg
     1681 cgcttgatgg gttcaatttc ttcacagact gctattttca atttttatcc gtgatggaaa
     1741 aaacgtctat cgtgctccct cgcggtcaac tcacacacgt tctgcgccat acgtttgcgg
     1801 cgcacttcat gatgtcgggt ggaaacattc tggccttaca aaaaattctc ggacaccacg
     1861 atataaaaat gactatgcgt tacgcacatc tggcaccgga tcatctggaa acggcgctcc
     1921 gtttcaatcc tctggcaacg ctgccaagtg gcgacaaagt ggcggcagcg gttggcatta
     1981 ccccgtaata accaccactg accaccaacc caacttattg tttttactct aacttattgt
     2041 tttcattaac ccgtttacat aaatgggttt tttgttatct aaaatttatc tactaccctg
     2101 caaccctctc aaccatcctc aaaatctcct cgcgcgatag cggcttacgg tcggtgacaa
     2161 aatgcagcgt catcccctca ataaacgcat caagcgcgcg ggcggttccg ggttcaaacc
     2221 attgttcgag cgtttgctga ctgcgctgca tccagttttg cattaccgtt tttaatagcg
     2281 gttttcggct agccagcgcg tagagctggt acatcagctc catgttatcc ggcgttgcaa
     2341 cctgtgagct gtagatcata tcggtgatag cctggcatgc gccttgagca tcactaacat
     2401 cgctaaaaaa tgcctgatat tgccgggaca taatctcagt aaaactgctg aacgcctcca
     2461 gcaacaactc atcaattcct gaaaaatagt aggtcatcga tcccaacggt accccggcaa
     2521 gggtagcaat tttgcggtgc gtaacagcat gtattccgta aagtttcacc gcctccagcg
     2581 tggcctggat aattttttct cgccgttgcg gatcgttagc gcgacgcatg tcatttcctc
     2641 tgtctgcaat tgtgtacaaa tgtacacaac cttgctaatg ttgtcttccc tcttcttatt
     2701 ttgacgtgat ctatgaccgt aaattcttca cgtaatgcat tgaaacgccg aacctgggcg
     2761 ctgtttatgt tcttcttttt gccaggcctg ttaatggcgt cctgggcaac ccgtacgcct
     2821 gctatccgcg atattctctc tgtctcgatc gctgaaatgg gcggtgttct ctttggtctg
     2881 tcgatcggtt cgatgagcgg tattctctgc tcggcgtggt tagtgaaacg ctttgggaca
     2941 cgtaatgtca tcctggtcac gatgtcctgc gcattgatcg ggatgatgat attaagtctg
     3001 gcactctggc tgacatcgcc cctgctcttt gccgttggtc tcggcgtctt tggggcaagt
     3061 tttggttctg cggaagtggc gataaacgtt gaaggtgccg ccgttgagcg agaaatgaat
     3121 aaaacggttt tgccgatgat gcacggtttt tatagcctgg gcacgctggc aggcgctggt
     3181 gtcgggatgg cactgacggc ctttggcgtt ccggcaacgg tgcacatttc attggcggcg
     3241 ctggtaggta tcgcgcctat ttatatcgcc attcaggcaa tccctgacgg tacgggcaaa
     3301 aatgctgccg atggcaccca gcatggcgaa aaaggcattc ctttttatcg cgatatccag
     3361 ttgctgttga ttggtgttgt agtgctggcg atggcctttg ccgaaggttc tgccaacgac
     3421 tggttaccct tattaatggt tgacggtcac ggttttagcc ctacttccgg ctcgctgatt
     3481 tatgccggtt ttaccctggg gatgaccgtt ggacgcttca ccggcggttg gttcatcgac
     3541 cgttacagtc gcgttgccgt ggttcgggcc agtgcactaa tgggggcgtt gggtattggg
     3601 ctgattattt ttgtcgatag cgcctgggtc gcaggagtgt ctgttgtact ttggggactg
     3661 ggtgcctcgc tgggcttccc gctgaccatt tctgccgcta gcgataccgg ccccgatgca
     3721 ccaacccgcg tcagtgtggt agcaacgacc ggttatctgg ctttcctcgt cgggccgccg
     3781 ctgctgggct atctcggcga acattatgga ttacgtagtg caatgctggt tgtactggcg
     3841 ctggttattc tcgcggctat tgtcgcgaaa gccgtcgcca aacccgatac caaaacgcag
     3901 acggcgatgg agaatagttg atgagcatta aattaattgc ggtagacatg gatggtactt
     3961 tcttaagcga tcaaaaaacc tataaccgtg agcggtttat ggctcagtat cagcaaatga
     4021 aagcacaagg cattcgcttt gtggtcgcca gcgggaatca atattatcag ttgatctctt
     4081 tcttccctga aattgcgaat gaaattgcct ttgtggctga aaacggcggc tgggtagtga
     4141 gtgaaggcaa agatgttttt aatggcgagc tgtcgaagga tgcctttact actgtcgtgg
     4201 aacatttgct gacgcgcccg gaagtggaaa ttattgcctg cggaaaaaat agcgcctata
     4261 cgctcaaaaa atatgacgat gccatgaaaa cggtggcgga aatgtattat caccgtctgg
     4321 aatacgtcga taactttgac aacttagaag atattttctt taagtttggc ctgaatcttt
     4381 ccgatgaact gattccacaa gtacaaaaag cattacatga ggccatcggc gatattatgg
     4441 tgccagtcca caccggcaac ggcagcatcg atctgattat ccccggcgta cataaagcca
     4501 atggccttcg ccaactgcag aaattatggg gaatagacga cagcgaagtg gtggtctttg
     4561 gcgatggcgg taacgatatt gagatgctgc gtcaggcagg ctttagtttt gcaatggaaa
     4621 atgccggcag cgcggtcgtc gcagcggcaa aataccgggc aggctccaat aaccgtgaag
     4681 gcgtactgga tgtgatcgat aaagtgctta aacacgaagc gccatttgac caataaaaac
     4741 ggcagcacgc ccggcaacac aatacctgaa tgaaggctgc ataattcgcg gaaattaacc
     4801 cgctgcatta tttcaggcga aatgattatg aaaaattgtc tactgttggg cgcgctttta
     4861 atgggcttta ctggtgtggc gatggcgcaa agtgtcaccg tcgatgtgcc gagtggatac
     4921 aaagtggttg tagttcctga ttcagtcagc gttccacaag cggtcagcgt cgcgactgtg
     4981 ccgcaaaccg tctatgtggc tcctgcgcca gctccggctt accgcccgca tccttatgct
     5041 cgccatcttg ccagcgtagg tgaagggatg gttatcgaac accagattga cgaccatcac
     5101 cattaattcg agaatgcctg atcgcacaaa gcagtcaggc atttttttac ccttcgtgag
     5161 aatttcccat ctgtttatct tttaaaaaga taaccatcag cgacagccac aaaattccgt
     5221 tgacaaggtt gaagagatta aactgtccgt tgcccccgtt cagccaggca tgtttgctga
     5281 tttcaatacc aacggtaaag atcagcattt gcagcattcc catcgccgca gaaaccgtac
     5341 ctttactcat atcgctggca aacagggtta atcgcaccag tcccgcattc gccagaccaa
     5401 taccgaaagc ataaatactt aacccggcag tcatccataa atacgcgtgc gatgagataa
     5461 ccgttgccgc agcagcgacc aacagaccaa tcataatcgg ccagccgccc ataattatca
     5521 gcgaacgtac ggtgcggcgc gaggtcaaac gcgctaacaa caagttaccc gcaattaacg
     5581 ccccgaaaat aggcacttgc agcaagccat attcatagct gctcaactgc tcgccggtaa
     5641 tgatgataat cggcgactgg gcgatccacg ccagcaatgg caggctaacg aatcccagcg
     5701 ccagcgcccc cgccacaaag cggccgttct tcagcaccag cttatagtca cgaccgagtt
     5761 ctttcagtga cagtttctcg cctatacgcg tggcggtttc aggcatggct cgttgcagac
     5821 cgaaaaagga gatagctgcc aatgcggcaa acaaaacaaa catcccctcc cagggcagca
     5881 catggatcca cgccgcgccc accagcggac caagtagcgg agcaatcagc gccacgttcg
     5941 ccatcagcgc ggtgatcttg atacaaaccg cctcttcgaa ggattcctga attgcggcgt
     6001 atcccacagc gccaatgaaa cagaggctta tgccctgcaa gaagcgcaac agggtgaatt
     6061 gttcaatgtt ttgcgccagc aatattgcca gacaggtgat gataaaccac accacccccg
     6121 ccagcatcac cggacggcga ccaatacgat ccgacagcgg ccccaggagc cattgtaaaa
     6181 acatcccgcc cgccagatat gcggtcattg aagtaggaac ccaatcaatg cccgcctgat
     6241 attgttccac cacggccaac ataccgggtt gaatcatatc gttgccgata taggttgaaa
     6301 attcgtaaag caccagacag agagggaaaa gtaacgcctg acgtccaagc ctggcaccgg
     6361 aagctaattt attttgcatg caatttcttc gccaataata atcgcgcaga gtttaataaa
     6421 agcgcagcta acgagaaagc gaattttgta gctgaaacca cggttaagca cattcttaca
     6481 ttattgcgag tatagctacg ctttctttaa gttttattta acctatgccc gttacaatca
     6541 cccaccgtaa acaggccgct tgagggaaat aagacgatgc cgctttaccc agtttaacct
     6601 gcactttatt ctcaacgact tgcctgtatt ggctcccttt taatcacttt gcgtcgggaa
     6661 gttatgctgg aaaatttgaa tctctctcta ttctctctta ttaacgcgac gccagactcg
     6721 gctccgtgga tgatctcgtt ggcgattttt attgctaaag atttgattac cgtggtaccg
     6781 ctgctggccg cggtgctttg gttgtggggg cttacagcac aacggcaact ggtgataaaa
     6841 atcgctatcg cgctggcggt cagcctgttt gtgtcctgga cgatgggaca tctttttccg
     6901 cacgaccgac cctttgtcga aaatatcggc tataacttcc tgcatcatgc ggcggatgac
     6961 tcattcccaa gcgaccacgg tacggtgatt ttcacctttg cactggcatt tttatgctgg
     7021 catcgcctgt ggtccggctc acttttaatg gtgctggccg tcgtcattgc ctggtcgcgc
     7081 gtttatcttg gcgtccactg gccgctggat atgctcggtg gattgctggc aggtatgatt
     7141 ggctgcctta gtgcccagat tatctggcaa gcgatggggc ataaactcta tcaacgtctg
     7201 caatcgtggt atcgcttctg ttttgcatta ccgatccgca aaggctgggt gcgtgactga
     7261 tctctgctaa aaagtgtagt attgagcggc tcgcttcaat aactattcag agggattatg
     7321 gaaacacgtc gcgaagagcg tatcgggcag ctgctgcaag aattaaaacg cagcgataag
     7381 ttacatctta aagacgccgc cgccctgctt ggggtttcgg agatgacgat tcgtcgcgat
     7441 ctgaacaacc acagtgcgcc cgtcgttttg ctcggcggct atattgttct ggaaccgcgc
     7501 agtgccagcc attacctgtt aagcgatcaa aaatcccgcc tggtggaaga aaaacgccgg
     7561 gcggcaaaac tggctgcgac gctggtagaa cccgatcaga ccctcttttt tgactgtggc
     7621 accaccacgc cgtggattat tgaagcgatt gataatgaaa tcccttttac cgccgtttgt
     7681 tattcgctaa atacctttct ggcgctgaaa gagaaacccc attgccgcgc gtttctttgc
     7741 ggtggtgaat ttcacgccag caacgccatt ttcaaaccca tcgattttca gcaaacgctg
     7801 aataattttt gcccggatat cgctttttat tctgcggcgg gcgtgcatgt cagtaaaggc
     7861 gctacctgtt ttaatcttga agagttgccg gtaaaacact gggccatgtc gatggcgcaa
     7921 aagcatgtgc tggttgtcga ccacagtaaa tttggcaagg tgcgtccggc gcgcatgggt
     7981 gacctgaaac gctttgatat tgtggtgagc gattgttgcc cggaagatga gtatgtgaag
     8041 tacgcgcaga cgcagcgcat taagttgatg tattaatgac gtataaccgg atgacgtttc
     8101 gcgccatccg gttatcagaa gattaagaga accagctgcc gaaccactga tggaatttca
     8161 tcatcacgaa atcccacatc cgaccaaaga atccgccctc ttccacattt tccatcacga
     8221 tcagcggacg ctgctcaatg gatttaccgt taagctggaa atcaatggtc ccgacaacct
     8281 gacctttttt cagcggtgcg gtaagctgcg gttccgttaa cgtataactc gctttcaggt
     8341 ttttcagctg cccacgcggt atggtcactg agcccgcttc acctgccccg agattcactt
     8401 cgctcttatc accaaaccag acgcgctgag tcacaaaggt ggcatcaggt ttaattggcg
     8461 tcacggtttc aaagaagcgg aaaccccagg tcaataattt ctcagactca ttaaaacgga
     8521 tacggtcagt tttcgccccc agcactacgg agattaaacg catatcaccc tgggtagccg
     8581 aagcaaccag attatatccc gcacctgccg tggttcctgt cttcatgcca tcaacattca
     8641 gattgctgct ccacagcaga cggttacggt taggctgacg aattttgttg aaggtgaact
     8701 ctttctcttt atgaatggcg tactcttccg gcacatcgtg gatcaatgct ttacccagca
     8761 atgccatatc gcgcgcggta ctgaactgcc ccggcgcatc caggccgtgc accgtctgga
     8821 agatagtgtt ggtcagcccc agttttttgg cataaccatt catcagacca ataaaggact
     8881 cctggctccc ggcaacgtaa tcggccagcg caatacaggc gtcattaccg gactggataa
     8941 tcacaccttt gttcaagtct gccaccgaaa cctgatcgcc cggtttgagg aacattaccg
     9001 atgaaccacg cagtgccgga tttcccgtcg cccaggcatc tttaccgacc gtcaccatat
     9061 cggtgagttt aatcttatcg gccttaagcg cctgcccaac cacatagctg gtcatgattt
     9121 tagtcaggct cgcgggatcc agtttctcat ccgcgttgcc ttctgccagc actttaccgc
     9181 tggcgtaatc cattaaaatc catgcacgcg catccacgct cggcgcttca acggtttgtt
     9241 ccgccgcgaa tgccgttggg gcaaaaagga ataaaaatgc agaacccgct gcaagaccac
     9301 gaaggagaga ggagtattgc gtcataaatg ccacccgagt atccattcaa aaaaaatacg
     9361 ttatatcgcc gtatgataag ttcggtgatt aataacgcac acacaaactt aaagaaaccg
     9421 taagttggta aagtttttaa agtttatgca atatcctgcc gcttcgctgc ttctggtgtc
     9481 atttttttgc cagctgttgc ttaaaagtta actttacttc accatcggag tcctgttcag
     9541 cctgtggctg cctgtcggaa aaggggtgag tatgattacg ctgtggggtc ggaataattc
     9601 aactaacgta aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat
     9661 tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa
     9721 cgggctggtg ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc
     9781 cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc
     9841 acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac cagacactca gtaatgctca
     9901 tcgcgggatc ctgatgggat tagtcagaac accacccgaa gagcgcgatc aggccgccat
     9961 tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa
    10021 agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat
    10081 ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta
    10141 tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt
    10201 gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc
    10261 gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc
    10321 ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc
    10381 tataaataat ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct
    10441 cacagcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc
    10501 tttcgcttcc ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc
    10561 gtagtttttg cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt
    10621 cagccacagt gcattactcc acggattcat cgccattcct tgcggattac gaatgccata
    10681 agaccagatc tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc
    10741 gccctggtcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg
    10801 gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg
    10861 cccgccaaaa tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc
    10921 ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc
    10981 atcatcgcca acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc
    11041 taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag
    11101 cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat
    11161 taacataccg tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg
    11221 cagtacttcg acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag
    11281 gggcacaagg aaaaagaatt gtcgatgcat aacgcctccg gattgactct ggcttaaagc
    11341 gtagtcagtg gaggagataa caaattcatt tttacaaaaa cttaaacatg aagggggaga
    11401 cgctttctcc cccttagttt tcaggccttc tcaagcatgg cgtgcttctg caggctctgg
    11461 atactcagcg ttaagctcat cagacaattt tcaagcttat cggcgttgac ggtaataact
    11521 gtcgggcaat catggtgccc actcatcaaa catactgcgg ctgtcgctaa tgcttcttca
    11581 gcatgatgaa gagcactcca ctcttcctga tccagatgaa gattcaaccg cagcgattta
    11641 tcgtgcagtt cgcgattcag tttaaaaaag ttatctcgta gatgattgct ttcgctgacg
    11701 gacatgtatc cttttgcctt cctcagttgc acgtatgcag cgaggtcaac gcgtgcgtcg
    11761 atcaaccggt tcagcagatc gattcgctgt ctgtcaacga acatacttcg atcctcctct
    11821 ttccagcctt gttgcaatta aatagtatgg ctattttttg agcaaaatgc tgtccaggat
    11881 caattcttca cgccaaatta acaaaattta catttccttc gcctccgcca gcgaatgaga
    11941 ttgcgataca aattttatcc agaactctgt agaatccctg ccctgacctt tcctgtaact
    12001 gaatattttt taagctatga gcaaagtaac tccccagccg aaaatcggct ttgtttccct
    12061 tggctgtccg aaaaaccttg tcgattcaga gcgtattctc accgaactac gcactgaagg
    12121 ttatgacgtg gtaccgagct atgacgatgc ggacatggtg atcgtcaaca cctgcggctt
    12181 tattgacagc gcggtacaag aatcactgga agccattggt gaagcgttga atgaaaacgg
    12241 caaggtaatt gtgaccggtt gtctgggggc aaaagaagat cagatccgcg aagtccaccc
    12301 gaaagtgctg gaaatcaccg ggcctcatag ctatgagcag gttctggagc acgttcatca
    12361 ctacgtgcca aaaccgaaac acaacccatt cctgagcctg gtgccagaac aaggtgtgaa
    12421 actgacaccg cgtcattatg cctatctgaa aatttcagaa ggctgtaatc accgctgcac
    12481 cttctgcatt attccgtcta tgcgcggcga cctggtgagc cgtccgattg gcgaagtgtt
    12541 aagtgaagcg aaacgtctgg tagatgcggg cgttaaagag attctggtga tctcgcagga
    12601 tacttccgcc tatggcgttg atgttaaaca tcgtactggc ttccacaacg gcgagccggt
    12661 aaaaaccagc atggtcagcc tgtgcgaaca gttatcgaaa ctggggatct ggacacgtct
    12721 gcactacgtt tacccttatc cgcatgtgga cgacgtcatc ccactgatgg cagaaggcaa
    12781 aatcctgccg tatctggaca ttccgttgca gcacgccagc ccgcgcattc tcaaactgat
    12841 gaagcgtccg ggttctgtag atcgccaact ggcgcgcatc aaacagtggc gcgaaatctg
    12901 cccggaactg accctacgct caacctttat tgtcggcttc cctggcgaga cagaagaaga
    12961 tttccagatg ctactcgact tcctgaaaga agcgcgtctg gatcgcgttg gctgctttaa
    13021 atacagcccg gttgaaggtg cagacgccaa tgccctgcct gaccaggttc cggaagaagt
    13081 taaagaagaa cgctggaacc gtttcatgca gctgcagcag cagatttccg ccgagcgcct
    13141 gcaagagaaa gtgggccgtg aaattctggt gattatcgac gaagtggacg aagaaggcgc
    13201 gattggtcgc agcatggcag atgcaccgga aatcgacggc gcggtttatc tcaatggtga
    13261 aaccaacgtt aagccgggtg atatcctgcg tgtgaaagtc gagcacgccg atgagtacga
    13321 tttgtggggt agccgggttt aattaattca ggccgggcaa aatcatttgt ccggcctgcg
    13381 aataatgatt actgccagct aatctgagcc ctgccgtttt tcttactctt atacagccgc
    13441 tcatccgcca gcattttcaa gcgttcgacg gtggtacatt catttgaatg cgctacaccg
    13501 atactggtac ttaaattaat gcttcccacc tggaaatgaa cgatttcttt tagaatcgat
    13561 agcaactctt ccagcgttcc acccgtataa atcaccgcaa attcgtcacc gccgaagcgg
    13621 tagattttac ctttatctac cgggatctgt gatttaagga ttttcaccac tttcatcaac
    13681 acttcatcgc ccagcaaatg accgaaggtg tcgttaatgt ttttgaattt atcgacgtca
    13741 aacaggcaca aataaccgcc tttttccggc agcgctttta aatcttcgtc aaaggatttt
    13801 ctgcccattg ctttggtcag gtcgtccagg aacgccgagt tatacatact gtagagtttt
    13861 ttgacatcgg cagccgaaaa gaaaagcccg ccatccagta cgcgttcaaa atggttatta
    13921 atgatggctt ctttctccac aataccacga ttaatcactc tgcgtaccag atagaggcaa
    13981 ctggtacccg atatcactgc caccaatgcg gcaaagaaaa taatcaatgt acagacccgc
    14041 aaataaacat cttttttata cgcaaatacg tacaatccgg tcggcaaata ggcgaagtgc
    14101 tccatcgctt ccgaaacggt gaagatggcg tttttagcat tatattcatc accggtcacc
    14161 ggtttaccgt tcttaaatag agagaacatc aggttctcgc tgtccatcgc cagcgatttt
    14221 gacagtaaag aaaaacgctc cagaccaagc aaaaatatca gactacgatt gtgagttacc
    14281 ggatagaggt aatagacgaa ggttttaccg tcaaaagaga taaaccctgt atttttgaca
    14341 tgtcgatact tcacaacctg cataatatct gcacaataat ttttctcgaa ctcctttacc
    14401 tgaatacatt cgtcactgga aatcagataa ggaccgacat cgtacaaata agagggggaa
    14461 accgagtcgg cgtaagctgt gcgcaaacca accaacagct gttcctccgt gggatttagc
    14521 gtgttgataa gatcaattgc cacgctacgc cgatattcag caatatttgc caccgcaaaa
    14581 gcagtatcaa taacggactg tttcaggcta tacacccgtt cctttaccat ttgagtgtag
    14641 atcccgcagg caactgctga aatcatgata aaaatcagca gactgactgt aagtacgaac
    14701 ttattgattc tggacatacg taaactactc ttttactaat tttccacttt tatcccaggc
    14761 ggagaatggc atgggtttct ggtacagata cccctgagcc tgaacacatc ctaatttaat
    14821 taattcgcgc gcaacgtctt cgctctctac gccttccgca atcaccggga tccggttata
    14881 gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca gtaccgaaaa tatcgccggt
    14941 aaattgcgag tccaccttta tgctgtctgg attaatttca taaaaacgtt tcaggtttga
    15001 caaaccggtg ccaaagtcat caatcgatat ccgtactcct tgtttacgca gttgttcaat
    15061 attgagataa aaatgttcat taattcgctc gatcacggtt tcagtgatct caacacagag
    15121 gctgttcgcc ttcaggccat tttctttcat gatggtggta aaacgctcat gaaagtgcgg
    15181 ttcgtttaac tgccagggcg aaacattaat atgcagtaag aaatcatcct tcagcgacgc
    15241 acgctgacta aaatgtctga attcacccat tgccgtttta ataatctgat aacccagctc
    15301 attgataaac ccgatatctt cagcaatggt aatgaacttc agcggtgaaa tgatcccccg
    15361 ctccgcagaa acccaacggc acaacgcctc cgcgcccaga atggcgcggg tgttaatatc
    15421 aacgatcggt tgatagacga ggaatagttc gccaatttcc attgcatggc gaatattacg
    15481 cgcaatccag atattctctt ctatttcatt gagtttcgcg tcatcacaga aaataaactc
    15541 ggagaacata tggtcacgga tactggaaat cgccagtttc agtttttctg cgtaactcca
    15601 ggcgttttcc ccctgcaaag gttcacaaat ggcagactta tggatgcgca gattgcggta
    15661 gccaaagctg ctgtcaaaat cgtttaccat cgctatttta cgggtaaatg tctgtacatt
    15721 ttcccgacac agaagtaaat agagatcggc attgaatgtc acgatatcgc agcattgcgc
    15781 atatttctcg gcaattcgct gacacaattt cctgacgatt ttattgcgtt catcaacacc
    15841 aaacaccgct tccacggctt ccagaccaca cagtttgatg atccccacag ccagatactg
    15901 attattgttg gactcaataa tcgccatatt tttaataaaa ccgctttcct gatcgtaaga
    15961 gatcagtgag agcaatttat ttgaccgttc aatttcggca tctttcattt ccgcgatcga
    16021 atttttgatg ctgataatct ctttcgcatc gccttcgcga attttcagag cctcgatctg
    16081 atccgttgag tcgggcaaaa acgccagcga attaaccagc tcggtcattt cagaaaattg
    16141 ccgacggaac acacgagtgg tcatgaaata taccaccagc aacacgataa taatgaacgc
    16201 aaacggtaga taaaaaagtt tatctgatgc gttcaataaa ttaccgccag gataataaaa
    16261 atagatttcg aacaatgcat tatttggcac tgctgatgtc tttgcaatat actccgtatc
    16321 gacagaatca ataatattat aattaccttc gctattataa ataatcagtt tttcacgagc
    16381 gagttgattc ttggcaatga tctcatgacc ttgtttcaaa aatagaactt catcattagc
    16441 gctgatttga tcaagatact cgttcagata ccgccagttt aaatcgacat gaattttatc
    16501 taccgctaac aaatcgtgat ctttgccgtt gatacgtagc gtaaaagaga tacatttttt
    16561 acccggacga tcaaagcaat tgtatgccgg attccagctg ttgttagagt catctgaagc
    16621 caccgaccac ggtcgtttcg atgggttaaa atcggcaaaa ataacccgcc ctgactcgtc
    16681 gacaagcggt ccatttactt gttcctgatg ataaacaacg attttatcat tcgccctccg
    16741 tgaaaagaga taatatttat tatcatcaag taccagtccc accgagtcta aaaatggcat
    16801 catttccatt ttcagaatca cggc
//
LOCUS       DAZKIV010000102        16751 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZKIV010000102 DAZKIV010000000
VERSION     DAZKIV010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.102"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1435
                     /locus_tag="ACHOT6_003210"
     CDS             <1..1435
                     /locus_tag="ACHOT6_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9948380.1"
                     /translation="NQSATDTLSSPVPETDAVETAIPDNEKTECKVEVEPSVEREGPF
                     YFLFTDKDCEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNANTAQNS
                     EQPEPVKVTADEVKKIMQAANISQPDANQLLAASRGEFVAGISDPNDPKWVKGIETRD
                     SVNQNQQETEQNDQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPD
                     CGAVMGDATYQEIFDEENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASD
                     PLIKANSHHNLTSTSRAGIHLMIDLETIGKNPDAPIISIGAIFFDPQTGDMGPEFSKT
                     IDLDTAGGVIDRDVIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVQV
                     WGNGANFDNTILRRSYERQGIPCPWRYSNDRDVRTIVELGKAVDFDARTAIPFEGERH
                     NALDDARYQAKYVSTIWQKLLPNQADF"
     gene            1500..1748
                     /locus_tag="ACHOT6_003211"
     CDS             1500..1748
                     /locus_tag="ACHOT6_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFP9948381.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            1726..2856
                     /locus_tag="ACHOT6_003212"
     CDS             1726..2856
                     /locus_tag="ACHOT6_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFP9948382.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(2902..3540)
                     /gene="ompW"
                     /locus_tag="ACHOT6_003213"
     CDS             complement(2902..3540)
                     /gene="ompW"
                     /locus_tag="ACHOT6_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFP9948383.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            3780..3869
                     /locus_tag="ACHOT6_003214"
                     /pseudo
     CDS             3780..3869
                     /locus_tag="ACHOT6_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            3897..4640
                     /gene="yciC"
                     /locus_tag="ACHOT6_003215"
     CDS             3897..4640
                     /gene="yciC"
                     /locus_tag="ACHOT6_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFP9948384.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            4670..5209
                     /gene="yciB"
                     /locus_tag="ACHOT6_003216"
     CDS             4670..5209
                     /gene="yciB"
                     /locus_tag="ACHOT6_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFP9948385.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            5314..5712
                     /gene="yciA"
                     /locus_tag="ACHOT6_003217"
     CDS             5314..5712
                     /gene="yciA"
                     /locus_tag="ACHOT6_003217"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFP9948386.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(5752..6471)
                     /gene="tonB"
                     /locus_tag="ACHOT6_003218"
     CDS             complement(5752..6471)
                     /gene="tonB"
                     /locus_tag="ACHOT6_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFP9948387.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            6695..6991
                     /gene="yciI"
                     /locus_tag="ACHOT6_003219"
     CDS             6695..6991
                     /gene="yciI"
                     /locus_tag="ACHOT6_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFP9948388.1"
                     /translation="MLYVIYAQDKADSFEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            7290..8543
                     /gene="kch"
                     /locus_tag="ACHOT6_003220"
     CDS             7290..8543
                     /gene="kch"
                     /locus_tag="ACHOT6_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFP9948389.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(8598..8771)
                     /gene="yciY"
                     /locus_tag="ACHOT6_003221"
     CDS             complement(8598..8771)
                     /gene="yciY"
                     /locus_tag="ACHOT6_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFP9948390.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            8914..10374
                     /gene="cls"
                     /locus_tag="ACHOT6_003222"
     CDS             8914..10374
                     /gene="cls"
                     /locus_tag="ACHOT6_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFP9948391.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            10409..10738
                     /gene="yciU"
                     /locus_tag="ACHOT6_003223"
     CDS             10409..10738
                     /gene="yciU"
                     /locus_tag="ACHOT6_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFP9948392.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(10791..11795)
                     /gene="oppF"
                     /locus_tag="ACHOT6_003224"
     CDS             complement(10791..11795)
                     /gene="oppF"
                     /locus_tag="ACHOT6_003224"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFP9948393.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDSL
                     "
     gene            complement(11792..12805)
                     /gene="oppD"
                     /locus_tag="ACHOT6_003225"
     CDS             complement(11792..12805)
                     /gene="oppD"
                     /locus_tag="ACHOT6_003225"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFP9948394.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPCRLRACFKPVE
                     ELL"
     gene            complement(12817..13725)
                     /gene="oppC"
                     /locus_tag="ACHOT6_003226"
     CDS             complement(12817..13725)
                     /gene="oppC"
                     /locus_tag="ACHOT6_003226"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFP9948395.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(13740..14660)
                     /gene="oppB"
                     /locus_tag="ACHOT6_003227"
     CDS             complement(13740..14660)
                     /gene="oppB"
                     /locus_tag="ACHOT6_003227"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFP9948396.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(14746..16377)
                     /gene="oppA"
                     /locus_tag="ACHOT6_003228"
     CDS             complement(14746..16377)
                     /gene="oppA"
                     /locus_tag="ACHOT6_003228"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFP9948397.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
BASE COUNT         4365 a         4084 c         3987 g         4315 t
ORIGIN      
        1 aaatcagagc gcaactgaca ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg gacaacgaaa aaaccgaatg caaagtggaa gtcgaaccat ctgtagagcg
      121 tgaggggccg ttctacttcc tcttcaccga caaggattgc gaaaaatacg gtcgcgcaaa
      181 caaactttct ggtctggata aggcgctggc tgccggggct actgaaatca caaaagaaga
      241 atattttgcc cgaaaaaatg gcacatacac aggcttaccg caaaatgcaa ataccgcaca
      301 aaattctgaa caaccagaac cggtaaaagt taccgctgac gaagtaaaga aaattatgca
      361 ggcagccaat atcagccagc ctgacgccaa tcagttgctc gccgcatcac gtggtgaatt
      421 tgttgcaggg attagcgacc cgaatgatcc gaaatgggtg aaggggattg aaacccgcga
      481 ttctgtgaac cagaaccagc aagaaacgga acagaacgac cagaaagcgg aacaaaacag
      541 cccaaatgcg ttacaaaacg agccagaaac gaaacagcct gaaccagtgg cgcaacagga
      601 agtggaaaaa gtctgcaccg cctgcggtca gaccggcggc ggcaactgcc ctgattgtgg
      661 cgcggtgatg ggcgacgcaa cataccagga aatattcgat gaagagaatc agcctgaagt
      721 tcaggaaaat gatccggagg aaatggaagg tactgcacat cagcacaagg agaacactgg
      781 cggcaatcag catcatgcca gcgatagtga aactggcgag gcgtcagatc ccttaattaa
      841 ggcgaacagt catcataatc tcacatccac cagcagagcg gggattcatc tgatgatcga
      901 ccttgaaacc ataggaaaaa atcccgatgc cccgattatc tcaataggcg caatattttt
      961 cgatccgcaa accggagata tgggaccaga atttagcaag accatcgatc tggatactgc
     1021 tggcggagtc attgatcgtg acgtcattaa atggtggctg aagcaatcac gtgaagcgca
     1081 gtctgccatt atgaccgatg aaatcccgtt agatgatgca ctactgcaat tgcgggaatt
     1141 tatcgacgaa aactccggtg agttttttgt tcaggtctgg ggtaatgggg ccaacttcga
     1201 caacacgatt ttgcgccgtt catacgaacg acagggtatc ccctgcccgt ggcgctacag
     1261 caacgatcgc gatgtacgca caatcgttga gctggggaaa gccgtagact tcgatgccag
     1321 aactgctatc ccattcgaag gtgagcgcca caatgcgctg gatgatgccc gttaccaggc
     1381 aaaatacgtt tcaactatct ggcaaaaact gctcccgaat caggctgatt tttaatgttc
     1441 aaccccggtc gttgcccacc agctatagtg gcggcgacca tgattagcga acgacgctca
     1501 tggcaagact tattctgctc actgagtggg caaaagagga attcagtgaa ccggtcccta
     1561 ctccgagtac gttaagtaaa tacgctaaag ccggaatgat atttcctctc cccaaaaaag
     1621 ttggaagacg ctggcgagtg gatccacaag ctcgctttgt cggaatggta aacaagccgg
     1681 aggtgatcgc cacagatcac cctgctttga agaggatact ggaagatggc gcgcccgcga
     1741 aaatataaaa ccgatgttcc gggattatct ccgtattttg acaaaagaaa taacaaagtt
     1801 tactggcgtt acaggcatcc cataacaggc aaaaatcacg gtctcggcag tattgaccag
     1861 aaactggcag aaactattgc agcagaagcg aacagccgtc ttgcccggca gcaaatggaa
     1921 caaatgctca gtctgcagga gaaaattatt agtgataccg gcggttcatc aaccgttacc
     1981 atttttctga ataattacag aaaaattcaa caggaaagat atgaaaacgg cgagatcaaa
     2041 ctcaacacgc tgaaacagaa agcggcccct ctcagggtat ttgatgaacg ttttggcacc
     2101 agaccgttag atgccataac cgtaaaagat gtggtatcag tacttgaaga gtacaaggcc
     2161 agaggacata acagaatggg acaaattttc aggaaagtac tgatcgatgt tttccgggaa
     2221 gctcagcaaa cgggcgatgt cccgccaggc tttaaccctg cagaatctgc aaaaaaaccg
     2281 caggtgcgga tatcaagaca gcgactgact tttgatgagt ggatgatgat ttataacgca
     2341 gcggaaaagg atggttactt tttacagcgc ggtatgctgc tggcactgat gacaggccag
     2401 cgcctttcag atatttgcaa aatgcaattt tcggatatcc gggatggtta tcttcatgtc
     2461 gaacagcaaa aaacaggaac ccggattgcc atccctctgg ctctgcgttg cgataaatta
     2521 aatctcaccc tagatgatgt ggtgtcatcc tgccgcgatt gcgttcttag tccgtggcta
     2581 ttgcaccacc atcacgcgaa agggacagct aagcgcggcg ggatggttaa gccagcaaca
     2641 ttaaccgttg catttaaaaa agcccgggat tctgtggatt acaactggcg tgctaatggc
     2701 accccaccct ctttccatga gcagagatct ttatcagagc gattgttcag agagcagggg
     2761 gttgatacca aaattttgct aggccattcg aatcaaaaaa tgaccgatat ttacaacgac
     2821 gcacgcggta aggaatggaa aaaactggtc atttgatgac cagttttgca gaggggtttt
     2881 gcagaggttt tgcagagaaa attaaaaacg atatcctgcg gagaacataa acacccacgg
     2941 atccagtcgt accgagtctt tcacggtagt aacaccagat ttatacttag ccgtggtatc
     3001 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     3061 ccccacctgc ccggcagctc cccaggaatc tttcagactg agatcggaaa gccccgcctc
     3121 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac cggccccaac
     3181 gtaaggacgg aatttgctgc tggcatcacc aaaataccac tgcgccatca gtgttggcgg
     3241 cagatggtga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     3301 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaagcc
     3361 cagttgagtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     3421 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     3481 aaaggcactg ccagaaagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     3541 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     3601 tgatcctaca tagatcacat tacatatgat aattcaccat ttattgatct ggattaattt
     3661 agaagttgca ttgagaatcc tggttaatgt gatttaaatc aatttttaaa tattttctct
     3721 acgcaaatat taactcgatc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     3781 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     3841 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     3901 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     3961 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     4021 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     4081 ggttgttcga cctggttcag aatatgtcac cggaacagca acaaattttg ctgcaggctt
     4141 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     4201 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     4261 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     4321 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     4381 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     4441 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     4501 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     4561 tggcgaacac cttgagcaac ctgatttctg ccatattgct catctatctg ttccgcctgt
     4621 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     4681 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     4741 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     4801 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     4861 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     4921 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     4981 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     5041 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     5101 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     5161 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     5221 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     5281 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     5341 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     5401 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc gaaagaaatt
     5461 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     5521 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     5581 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     5641 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     5701 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttctg cttactgaat
     5761 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     5821 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     5881 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     5941 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     6001 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     6061 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     6121 tacgggtttg atatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     6181 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     6241 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     6301 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     6361 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     6421 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgaagtcat
     6481 aatcatttca gtagaaaaac caggtctcga ttttaaatgc aaatagcaat catattcaat
     6541 aaggcaagac gatcaggatc agtgaaaatg tcatttttgg cgtaattgac cttactttat
     6601 gcatggtctt aacaaaaaca tcatctttcc gttgcagatc ttcgcccttt caaataacgt
     6661 actttacaac tttcccgaac aaggagttgt gcccgtgttg tatgttattt acgctcaaga
     6721 caaagctgat agcttcgaaa aacgcctttc cgttcgtccg gcacatttag cacgtttaca
     6781 gttactgcat gatgaaggtc gtttgttgac agctggtcca atgccagcgg tagacagtaa
     6841 cgatccaggg gctgcgggat ttactggctc aaccgttatt gctgaatttg aatcgctgga
     6901 agcagctcag gcctgggccg atgctgaccc gtacgtagcg gcaggagtct atgaacacgt
     6961 ttcagtgaaa ccatttaaga aagtgttctg aatagagatt caatatttaa gggcacggtt
     7021 tttgcaaccg tgtttttgtt tggcctttcc ttatagccgg tcagtgcatt caccaccaca
     7081 agcagataat aactttatct gtatttcttt atatgatgtg aacatattca ggaaaatgcc
     7141 ctttcctgac gcagagttgc tcgctcaaaa cctttgatta ctgccgataa agtcatctac
     7201 tattgtttaa cacattcacg cgctgcatgt atgttcagaa aatgtgcacg ctaagataat
     7261 taaacaaaat tgtggttaca gaggtatcag tgagtcactg ggctacattc aaacaaacag
     7321 caacaaattt atgggtgacc ctacggcacg acattctcgc gctggccgtc tttttaaatg
     7381 gattgcttat ttttaaaaca atctatggta tgtcggtcaa tttgcttgat attttccata
     7441 tcaaagcatt ttcagagttg gatctctcct tgctggcaaa cgccccacta tttatgctcg
     7501 gcgtctttct tgtcctgaac tccattggct tactgttccg ggcaaagctc gcatgggcaa
     7561 tcagtatcat tttgttgttg atagcgctaa tttacaccct gcatttttat ccctggctga
     7621 aatttagtat tggattttgc atttttacgc tggtgttttt gctgatactg cgcaaagact
     7681 tctcccacag tagcgccgca gccgggacaa tttttgcatt tattagtttc acgacattac
     7741 tgttttactc aacctacggt gcgctttatt taagcgaagg ttttaatccg cgaatagaaa
     7801 gtttgatgac cgcgttctat ttttcgatag aaaccatgtc aaccgtcggc tatggcgata
     7861 ttgtccctgt ttctgaatca gcacgattgt tcactatttc ggtcattatt tccggcatta
     7921 ccgtttttgc cacatctatg acctcaattt ttggtccgct tatccgcggg ggattcaaca
     7981 aacttgtaaa aggaaacaat catacaatgc atcgtaaaga tcattttatt gtttgcggac
     8041 attcgattct cgccatcaat acgatcctgc aactgaatca acgcggacaa aacgtaacgg
     8101 ttatcagcaa cttgcctgaa gatgatatca agcaacttga gcaacgctta ggcgataacg
     8161 ctgatgttat ccccggtgac agtaatgaca gttcagtatt aaagaaagcg ggaatcgatc
     8221 gttgccgggc cattctggcg ctgagtgata acgatgcaga taacgcgttt gttgtactct
     8281 cggcaaaaga tatgagcagt gatgtcaaaa cagttctcgc cgtcagtgat agcaaaaacc
     8341 tgaataagat taagatggta catccggata tcattctctc accgcaactg tttggcagcg
     8401 aaattctggc gcgagtttta aatggtgaag agattaataa tgatatgctc gtttcaatgt
     8461 tgttgaactc cggtcatggc attttcagcg ataacgatga acaagaaacg aaagctgaca
     8521 gtaaagaatc agtgcaaaaa tagagtttaa agacgaaaga gtaaacttat tgggcaccgc
     8581 aatggtgccc agtatattta gatattgtag atcgcaaaca ataacgagtt acgctgtttg
     8641 tttagaatgc acttcctgat gctgtggata cgcatatttc ggcgcgattg cccctcaagc
     8701 caacgcgatt tacgtcggct agcctgacgc tgcatgcgcc agcgccccac ttccgttcta
     8761 ctccgcttca tgttaactac tctatgcaat aacagaatgg tcattatacc cccacccccg
     8821 acgctgacca gtgcttttcc cgcgttttta tttgccagat gaatccatat gcgtaaactc
     8881 ataacaatgc gctttcaaaa ggatttctaa tctatgacaa ccgtttatac gttggtgagt
     8941 tggttggcca ttctgggata ctggttgctc attgcaggcg taactttacg cattctaatg
     9001 aaacgacgcg cagttccctc cgcgatggcc tggctgttga ttatttacat tctgccgtta
     9061 gtcggaatta ttgcctatct tgccgttggc gagctccatt taggcaaacg ccgcgctgag
     9121 cgcgccagag cgatgtggcc ttccaccgca aaatggctta acgaccttaa agcctgtaag
     9181 catatcttcg ccgaagaaaa tagcagtgtc gctgcgccat tattcaagct ttgcgagcgt
     9241 cgtcagggga tcgctggggt caaagggaat cagctacaac tgatgaccga gtcagatgat
     9301 gtgatgcagg cgttaatccg cgacatccag ctcgcgcgcc ataatattga gatggtgttt
     9361 tatatctggc agcccggcgg catggcggac caggtggctg aatcattaat ggcggctgca
     9421 cggcgtggta ttcattgccg attgatgctc gactccgccg ggagtgtggc ttttttccgc
     9481 agcccgtggc ccgagctaat gcgtaatgcc ggtattgaag tggtcgaagc cttaaaggtc
     9541 aatctgatgc gtgtgttttt acgccgtatg gacctgcgcc aacatcgcaa gatgatcatg
     9601 atcgataatt acatcgcgta caccggcagc atgaatatgg tcgatcctcg ctacttcaaa
     9661 caagatgcgg gcgtagggca atggattgat ctgatggcgc gtatggaagg ccccatcgcc
     9721 accgcgatgg ggattattta ttcctgcgac tgggagattg aaaccggaaa acgtattctg
     9781 ccgccaccac cagatgtcaa tattatgccg tttgaacagg ccagcggtca caccattcac
     9841 acaattgctt ctggccccgg ctttccggaa gatctcattc accaggcatt attgactgcg
     9901 gcttattcgg cgcgtgaata tttgatcatg accacgccct actttgtgcc aagcgatgat
     9961 ttacttcatg cgatttgcac ggcggcgcag cgcggggtgg atgtcagtat tatccttccg
    10021 cgaaaaaatg actcgatgct ggtcggctgg gccagtcgcg cattctttac ggaacttctt
    10081 gctgctgggg ttaaaattta tcagtttgaa ggcgggttac tgcataccaa gagcgtgctg
    10141 gtcgatggcg aactaagtct ggttggcacc gttaaccttg atatgcgtag tctgtggctg
    10201 aatttcgaga ttaccctggc aatcgacgat aaaggttttg gtgcagacct tgccgccgtt
    10261 caggacgatt atatttcgcg ttcacgtctg ctcgatgccc gtttatggct aaaacgtcca
    10321 ttatggcaac gtgtcgccga gcgactgttt tacttcttca gcccgttgct gtaaaacgtc
    10381 gccaacagag gttaaacagg tagtgactat ggatatggat ctaaacaatc gcctgactga
    10441 agatgaaacg cttgagcagg cctacgatat ttttctcgaa ctggctgccg acaatctcga
    10501 tccagctgat gttctgctgt tcaatcttca gtttgaagag cgcggcggcg cggagttatt
    10561 cgatccggcg gaagactggc aggaacatgt tgatttcgat ttgaacccag acttttttgc
    10621 cgaagtggtg attggtctgg cggacagtga agatggtgaa atcaacgatg ttttcgcccg
    10681 cattctgtta tgccgcgaaa aagatcacaa actttgccat atcatctggc gggaataaat
    10741 aatcgctttc ggcgtgaaaa agggctgaca actgtcagcc cttattgtgt ttaaagcgaa
    10801 tcgactttca ggcaagaaac ggcgtgtctg aaactcccct ccagaacagg acgtgttttg
    10861 gcgcactccg gaccggcaat cgggcaacgg gtacggaaaa cacaaccgga aggcgggttg
    10921 atcggcgacg gtaattcccc ttccagtaac tggatggttt tgttcttctc cagatccgga
    10981 tcaggtatgg ggactgccga catcaatgcc ctggtgtaag gatgtagtgg attgtggtag
    11041 acctcatcat aggtccccag ttctaccgca tggccgagat acatcaccaa cacacgatcg
    11101 gaaatgtgtt ttaccacggc caggtcatga gcgataaaaa ttaatgacaa tcccatctca
    11161 cgttgcagct gctggagcag gttgaccacc tgcgcctgaa ttgacacgtc cagcgccgaa
    11221 accggctcat cgcagataat cagcttcggt tcaagaataa gagcacgagc aatcccgata
    11281 cgctggcact gcccaccgga gaactcatgc ggatagcggt taatcaggtt aggcaataac
    11341 ccgactttca gcatcatcgc cttcacgcgc tcgcgaactt cctggcgtga cattttcgga
    11401 tgataagtac gcagtggttc agcgatgatc tcgccgatgg tcatacgcgg gtttagcgat
    11461 gccaacggat cctggaaaat catctgaata tcactgcgaa cggcacgcca ttcatcgggc
    11521 ttcatgccca gcaactcttt acctaaccag gcaacatgac cgtcggtcgc cttgaccaaa
    11581 ccgatgatgg cgcgagcaaa ggtggactta ccgcatcccg attcccctac cacacctaat
    11641 gtttcccctt catacaggcg aagagtgaca ccatcgacgg ctttgagcgt tttcggcggt
    11701 tgccagaacc actgtttgcc atctttgatt tcaaagtgca ctttaagatc ggcgatttca
    11761 aggaggactt ttcttccttc agttacagca ttcataacag ttcctccacc ggtttaaagc
    11821 aagcacgcag acggcaaggc gtaaactctt ccagcggcgg tgcgctacta caaatttcca
    11881 tcgcatgtgg acaacgtggc tggaacgggc aacctttcgg taatcgcagc aagtttggcg
    11941 gattaccagg gatggtcaac attgtttcac cttccgcatc aagacgcggc accgcgttga
    12001 gcaaaccgat agaataagga tgaacgggtt gataaaagac atcgcgcgca ttgccatatt
    12061 ccatcgtgcg cccggcgtac attaccagca ctttgtcgca gatccccgcc accacgccaa
    12121 gatcgtgggt gatcataatg atggcggtat taaattcccg cttcagttca tttaatagcg
    12181 tcatgatctg cgcctgtacg gtgacgtcca gcgcagtagt gggttcatcc gcaatcagta
    12241 gcttaggtcg acatagcaat gccatcgcaa tcatgactcg ctgacgcatg ccgccagaaa
    12301 attcgtgcgg gtacattttc atgcgtttac gcgcttccgg catttttacc gcatcgagca
    12361 tccgcaccga ctcttcaaac gcctcagctt tgctcatgtt cttatgcagc atcagcactt
    12421 ccattaactg ctcaccgacg cgcatatagg gattcaacga agtcattggg tcctgaaaaa
    12481 tcattgagat ttgttcagcg cgcagtttat tgagttcatg ctctggcaaa ttgaggattt
    12541 cacgcccatt gaaggttgcc gatccgccaa tacgtccgtt ggcagccaac aggcccatca
    12601 acgcaaatgc agtttgcgat ttacccgaac cagactcacc tacaataccc agcgtttctc
    12661 cggcacgtag ggaaaaattc aaatcattga ccgccgtgac gtcgccgtcc ggggtactaa
    12721 aggtgacacg caaatctttc acgttcagca gtgcgtcagc ctgttgttgt gcgagcggca
    12781 cagttgcagt ttcaattacg ctcatggctg cactccttaa cgatctttcg ggtcgagggc
    12841 atcacgcaag ccatcgccga taaagttgaa acaaaacagc gtcaccacga ggaatcccgc
    12901 tgggaacaac aataaccatg gagagacttc catcgagttc gcgccatcac tcagcaatgc
    12961 accccagctg cttaacggct cttgcgtacc caaccccagg aagctaagga aagattcaaa
    13021 gaggatcatg ctgggcacca gaagtgatgc gtagaccacc accacaccga gtacattcgg
    13081 cacaatgtgg cgaataacaa tgcccgatgt cgatacaccg ccaacttgtg ccgcctcaat
    13141 aaactctttg cgcttcagac tcagggtttg cccacgcaca atacgagcca tatccagcca
    13201 ggaaaccatg cctatcgcca cgaaaatcag caggatgttt tgaccgaaaa aggtcaccag
    13261 caaaatgacg aagaacatga atgggaagga gttgaggatt tccagcagac gcatcattac
    13321 cgaatccact ttaccgccca gataaccgga aagcgaacca taaagtgtcc cgacgaccac
    13381 agccaccagt gccgcagcaa caccgaccat gagtgagata cgcccaccaa tcgcaacgcg
    13441 cacaagcagg tcgcgaccgg atgagtcagt accaaagtag tgaccggact ccatatccgg
    13501 ggcgctggac atcatcgccc agtcagtatc gtcataggca aactgggaaa gcatcggtgc
    13561 caggattaca aataacgcga tcagcaccag cactatcaga ctggcaaccg ccgcacggtt
    13621 atgaataaaa cgtcgacgtg cgtcctgcca caagctgcgc ccttcgacct ccagcttttc
    13681 actgaaattt tccagcgtct cgctgttttt cttacttaac atcatcgcga gctccagtat
    13741 cagtaacgga ttttcgggtc gataaccgca tatagcacat cgacaatggc attaaacaaa
    13801 atggttaaag caccaaccag gatggtcagg cttaacacta aggaatagtc acggttcaat
    13861 gcgccattaa cgaacagttg tccaatcccc ggcaaaccat aaatggtttc gataaccata
    13921 gaaccggtaa taatgccgac aaatgcgggg cccatataag agagcacggg taacagagca
    13981 ggttttaatg cgtgacgtaa aatgatccgc cgcataggta accctttcgc ccgggcagta
    14041 cgaataaaat tggagtgtaa tacttcaatc atagagccac gggtaatacg cgcaatactg
    14101 gcgatataag cgagtgacaa cgccaccatc ggcaatatca tgaatttaag cgccccacca
    14161 ttccagccac cgcccggcag ccaatgcaaa atgatcgcaa atatcatgac taataatggt
    14221 gcaaccacaa aactggggat aacaaccccg gtcattgcca gccccatcac ggtatagtcc
    14281 catttggtgt tttgttttaa tgcggcaata acgccagcac taacacccag tattaccgca
    14341 aggaaaaatg ctgcggctcc cagtttggca gaaacgggaa aactggatgc aaccaagtca
    14401 ttgaccgaat aatctttata tttaaacgat ggaccgaaat caccgtgcgc cagttgtttc
    14461 aggtagctga aatactgtgt catgatcgga tcattaagat gatatttcgc ttcgatattg
    14521 gccatcactt ctggcggtaa agtacgttcg ccggtaaaag ggcttcccgg cgcgaggcgc
    14581 atcataaaga acgaaatagt aataagaata aatagcgtcg gaatcgcttc cagacagcga
    14641 cgtagaataa attttaacat tgccctacct tctggcctgt gccttctgta atgcgataaa
    14701 aatcagacac cgtggagcag gacactcctg ccccacgtat tgccattagt gcttcacaat
    14761 gtacatattc cgggtatagg tattatccag cggatctttg ccggtatagc caccaaccca
    14821 cggtttcacc agacgcgcat tcacgtagta ataaacagga acaatggccg aatccttatc
    14881 cagctgttgt tctgctttag tgtacagagc tgtgcgctgc gcctcgtcag tcactttcag
    14941 cgtttccgcc ataatgctgt caaaggccgg gctcttataa tgcgcggtat tcatcgagct
    15001 gttcgaaagc atggtgttca ggaaggaagt tggttcgttg tagtcagcac accagcctgc
    15061 acgggccaca tcaaaagtac cctggtgacg ggtgtcgagg aacgttttcc actcctggtt
    15121 aaccagtttg acgtttacac caatgttttt cttccacaat gaagaggcag caatcgccag
    15181 ctttttatgc agatcggagg tgttatacaa caggttgatg gtcaacggtt tgtctgcggt
    15241 ataacccgct tcagccagca gttttttcgc ttcttcgtta cgtttttcct ggctccagcc
    15301 aaaccattcc ggctgagtca attttgcgcc atcagtatac ggtggagtgt aaccataggc
    15361 gggcatgtcg ccctgcgctt tcactttatt aacaatgata tcgcggtcca tacctagttt
    15421 cagtgcggta cgcacacgca catcgttgaa tggcggtttc tggttgttaa tttcgtaata
    15481 gtaagtgcac aggtatggat caacgtgaac ttcgtccggg atctcttttt tcagcttctg
    15541 gaacaattcg atcggcatgt tgttataagt catgtcgatt tcaccactac ggtagcggtt
    15601 gacatcggta acttcagaag caataggcaa ataggttacc tggttaataa cggttttcgc
    15661 gttgttccag taggtcgggc tgcgttcaag aacgattcgt tcgtttacga cccaatcttt
    15721 taaggtatag gcaccgttgg tgacgatatt accaggctgg gtccattttt cgccgaattt
    15781 ctcgatagcg gcttttggca ccggtgaagt tgatgggtga acaagtaatt tatagaagta
    15841 cggaacgggt tcacttaagg tgacttctaa tgtgtgatca tcaatagctt tcacgccgag
    15901 atcggtaatc ggttttttcc cttcaagaat ttcatcaata ccggcgatat gcccatattg
    15961 cagataactg gcatacggag aagcagtgtt cggatcaaca gaacgttgcc agctatacac
    16021 aaagtcttgt gctgtgactg gcgtgccatc agaccatttc gcatctttac gcaaatggaa
    16081 ggtccagact ttcgcgtctt tattatccca ggattcagcg acgccaggtg ctggatgacc
    16141 gtcaagatcg ctgaccagta agccttcaaa caggtctcgg ctgatattag actccggaac
    16201 accttcaatt ttgtgcggat ctaatgactg aacttctgaa ccattgttac gtaccagtgt
    16261 ttgtttttcc gccagtgtga cgcctgcggg tacatcagct gccagcgcga cattccctgc
    16321 cattagcgca gccagaacgc cagctgctac taaacttctc ttggtgatgt tggtcattgt
    16381 tttttggact ccctcattat aattactggt attaaccagc atgtgtaatc ccggattggg
    16441 accctgcaca ggctcggaga ctttctgctg tcaggttact ttatcgttac gtattactat
    16501 caccgacttt atttattctg gtgattcaga cgatcacctt atgtcgattc gtactgtgtc
    16561 tcccctgtcg agacacatta ttctttcgcg acgctcttta attgttaatt attctcatta
    16621 ccaataaaga atcgtctggc ggttgcccgg aaagtaccaa atgcggttct tactcgccaa
    16681 tacattttgc aaaattgtta agcaattctc ttttattgac cgtatcagaa tctatagctt
    16741 gcatccatgc c
//
LOCUS       DAZKIV010000103        16546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZKIV010000103 DAZKIV010000000
VERSION     DAZKIV010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.103"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(123..659)
                     /gene="yghD"
                     /locus_tag="ACHOT6_003229"
     CDS             complement(123..659)
                     /gene="yghD"
                     /locus_tag="ACHOT6_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFP9948398.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGLSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETILQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(661..1839)
                     /gene="gspL"
                     /locus_tag="ACHOT6_003230"
     CDS             complement(661..1839)
                     /gene="gspL"
                     /locus_tag="ACHOT6_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFP9948399.1"
                     /translation="MSSILEIFFPLCASDTVRWQRRTPDVEHGIWPDVADEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVACKQIPTILPALLEEELNQDIDELHFAPLKIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGKP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1836..2813)
                     /gene="gspK"
                     /locus_tag="ACHOT6_003231"
     CDS             complement(1836..2813)
                     /gene="gspK"
                     /locus_tag="ACHOT6_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFP9948400.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDDGLYQKLKPLVCALPMTRQQININ
                     TLDVTQSVLLEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(2810..3415)
                     /gene="gspJ"
                     /locus_tag="ACHOT6_003232"
     CDS             complement(2810..3415)
                     /gene="gspJ"
                     /locus_tag="ACHOT6_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFP9948401.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNNAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGEQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQCGEIERIWLLRGPQLS"
     gene            complement(3412..3783)
                     /gene="gspI"
                     /locus_tag="ACHOT6_003233"
     CDS             complement(3412..3783)
                     /gene="gspI"
                     /locus_tag="ACHOT6_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFP9948402.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(3780..4343)
                     /gene="gspH"
                     /locus_tag="ACHOT6_003234"
     CDS             complement(3780..4343)
                     /gene="gspH"
                     /locus_tag="ACHOT6_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFP9948403.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(4347..4802)
                     /gene="gspG"
                     /locus_tag="ACHOT6_003235"
     CDS             complement(4347..4802)
                     /gene="gspG"
                     /locus_tag="ACHOT6_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFP9948404.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(4819..6042)
                     /gene="gspF"
                     /locus_tag="ACHOT6_003236"
     CDS             complement(4819..6042)
                     /gene="gspF"
                     /locus_tag="ACHOT6_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFP9948405.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPASTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPTMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(6042..7535)
                     /gene="gspE"
                     /locus_tag="ACHOT6_003237"
     CDS             complement(6042..7535)
                     /gene="gspE"
                     /locus_tag="ACHOT6_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFP9948406.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(7535..9595)
                     /gene="gspD"
                     /locus_tag="ACHOT6_003238"
     CDS             complement(7535..9595)
                     /gene="gspD"
                     /locus_tag="ACHOT6_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFP9948407.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEV
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREIVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(9625..10584)
                     /gene="gspC"
                     /locus_tag="ACHOT6_003239"
     CDS             complement(9625..10584)
                     /gene="gspC"
                     /locus_tag="ACHOT6_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFP9948408.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(10602..11012)
                     /gene="gspS2"
                     /locus_tag="ACHOT6_003240"
     CDS             complement(10602..11012)
                     /gene="gspS2"
                     /locus_tag="ACHOT6_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFP9948409.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(11078..11887)
                     /gene="pppA"
                     /locus_tag="ACHOT6_003241"
     CDS             complement(11078..11887)
                     /gene="pppA"
                     /locus_tag="ACHOT6_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFP9948410.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(12035..>16546)
                     /gene="sslE"
                     /locus_tag="ACHOT6_003242"
     CDS             complement(12035..>16546)
                     /gene="sslE"
                     /locus_tag="ACHOT6_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFP9948411.1"
                     /translation="TLLAGCDGGGSGSSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEP
                     TPDPEPTPEPTPDPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGFTFTP
                     GDKVTCVAGNNTTIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNALSLVT
                     SMNSCPANTEQVCLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPS
                     THTSPVVPVTTPGTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGVAGVNY
                     YTNSGRGVTGENGEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQ
                     LIHRYSTTGQNNTRVVPDDVRKVFAEYPNVINEIINLSLSNGATLGEGEQVVNLPNEF
                     IEQFKTGQAKEIDTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYKSV
                     SKFHVFHDSTNFYGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNEL
                     AYITEAPSIVRPENVTRETATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPNGYSWN
                     GGVNKDGQCTLNSDPDDMKNFMENVLRYLSNDRWLPDAKFSMTVGTNLDTVYFKKHGQ
                     VLGNSAPFAFHKDFTGITVKPMTSYGNLNPDEVPLLILNGFEYVTQWGSDPYSIPLRA
                     DTSKPKLTQQDVTDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNK
                     SVVNNDPQGYPDRVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWKYQQENKPDD
                     KPKLEVASWQEEVEGKQVTQFAFIDEADHKTPESLAAAKQRILDAFPGLEVCKDSDYH
                     YEVNCLEYRPGTGVPVTGGMYVPQYTQLDLGADTAKAMLQAADVGTNIQRLYQHELYF
                     RTNGRKGERLSSVDLERLYQNMSVWLWNDTSYRYEEGKNDELGFKTFTEFLNCYANDA
                     YAGGTKCSADLKKSLVDNNMIYGDGSSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLN
                     IKVDVEKYPGAVSEEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNANV
                     PVTVTVALADDLTGREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGNSST
                     NESASFTFTGVVKAPFYKDGAWKNDLNSPAPLGELESDAFVYTTPKKNLNASNYTGGL
                     EQFANDLDTFASSMNDFYGRDETSGKHRMFTYKALTGHKHRFTNDVQISIGDAHSGYP
                     VMNSSFSTNSTTLPTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYL
                     GKMNRVADDITVAPEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPEGE
                     LPKFFSDREGMKGWNLFQLMHRKARGDDVGDKTFGGKNYCAESNGNAADTLMLCASWV
                     AQTDLSEFFKKWNPGANAYQLPGASEMSFEGGVSQSAYNTLASLKLPKPEQGPENINK
                     VTEHKMSVE"
BASE COUNT         3616 a         4720 c         4205 g         4005 t
ORIGIN      
        1 gcgggttttt gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc
       61 tgggggaatt actctcgcca cgttaacgag agtaatttta ttgatactaa tctcctgata
      121 ctttaccccc gtccaaactc cagccgctgc acattcacca tcccagtctt ctcagcagca
      181 ctggcatcaa tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc
      241 agcaggtcat taaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct
      301 atccgcttga tgactaccga gtgcgcggaa gcactgtcac tgataacccg cgatacctgc
      361 gcaggcgttg ttgtggcgga ttttcgcgcc gcaataatat ccggcgcggc gctcctcagt
      421 ctggtgttca tcgccaccag ttgctgcaat attgtctcct gttgtactat ccgttcgctc
      481 aacggctgcc agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt
      541 aacatgccct tttcacgcgg agaaagcccc gccaggtgtt gtgccagcca gtgttcgcca
      601 cggcttaact ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg
      661 ttatttcctc cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaacgcgtc
      721 ctgttgcaca acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt
      781 tgctgcccgc aactggaggt gaagcgtctg gcgtttttga tcaaaggtga aaccacgcat
      841 ttcgatgtcg gaaagtgacg ctgatttcag ggtgctggct atcgctgaca actcggcgag
      901 cagccgggta tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg
      961 taaattcaca atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt
     1021 gcggctttgc gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag
     1081 cgcaaccaga atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg
     1141 gtagctgacg cgaggctgcc acggccctgt cagcaggttc ccttccggtt tgccataagt
     1201 ggtaacggcg ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga
     1261 tagcttttcc ggtgcaatgc cgactacggt tagcgaaagc ggtaaatcct gctcattgag
     1321 ctgggcgcgg aacatgaccg gagccagcgc cagcccggcg ctccatcccc ggcattcatc
     1381 gatgcggcag ataacccgtt gcgcatcgcc agccataaac ccacaaggaa tggacatcca
     1441 gtccggcgcg acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg
     1501 catatgctgt tggtgaatca ctgctacggt tgccagttgc tggtcgattt tcaacggggc
     1561 gaaatgcagt tcatcgatat cctggttcag ctcttcttcc agcaaggcgg gcagaatagt
     1621 cggtatctgc ttgcaggcga catcaggcag ttcaacctgc cagacgctga tccattcgcc
     1681 gggaatgtag agtcgaatcg catcggtttg cagccattgc tgaagctgtt cgtcagcaac
     1741 gtcaggccag ataccgtgtt ccacgtcggg tgtacggcgc tgccagcgga cggtatcgga
     1801 ggcgcaaagc gggaaaaaaa tctcaaggat ggaactcact cactttctcc tgtctgatgc
     1861 cagagaaccg aaaagtgttg tgggcccatg cggacaatta acgaattcat cgtcagttca
     1921 atctcattca cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg
     1981 gtttttagct gttttttagt acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac
     2041 tgatcgacat cttcccagcc cttcgccgga cgttgttgta ataatgcccg cgcctgaaca
     2101 gggcttaacc acgggtcaaa cagcgcctca agaagcacac tttgcgtgac gtctaaggta
     2161 ttgatgttga tttgctggcg ggtcatcggc agcgcacaga ccagcggttt cagtttttga
     2221 taaagcccgt cgtccattcc ctgcaccacg cgcatctcgc tgatatcggc cagcggttga
     2281 ttggcggcgt agaacggcac cgagcgggcg agatactcgc tgtcttcacg gcccagacgc
     2341 gtctgcacgc tgcggtcctc gtcaataaac tcccacaggc tttcggctat cagttcggcc
     2401 cgataagcag gcacatcgag gcgcgagatc agggcaatca gttgttgtac cgcgagcgga
     2461 cgcgacgccg ttgtgggctg agcgagggca ttcaggttaa agcaggcctg tgcgtcacgc
     2521 agagtgacgg cgatttgccc ctgcggcagt gggaaaaaac gagggccaga agcccacggt
     2581 tgtgccagat gcacgcgctt ttcatttttc aggctcagac tgagtgcact cagcgccagg
     2641 ctttctgcac tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc
     2701 ccaagttgtt gctgcatccg cccggaaagc gtgatggtta ccagcatcat aaccgccagc
     2761 aataccagca ccacaaccag tgccattccg cgttttggtg gtgaggtgat catgataatt
     2821 gcggcccacg taacaaccag atgcgttcaa tctcgccgca ttgcggcgaa tgcagagtca
     2881 tgcgcactgc cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtaccat
     2941 catagaactg caaacggagc gaatccgccg gaatcaactt ttgcattgtc ggcttcacgc
     3001 tgcctgcggc atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt
     3061 aaccgacggt gagcagattg ctgcgtggca gacgcatcaa cgggttaacc acgccgccgc
     3121 gcacaaagcg catgccttca ctctcagacg ccagcacgcc agcgcccgcc agtaacgctg
     3181 gttcacgctg gccctgttcg cctcttaccg gacgcggcat catttgtgtc agatcgtggg
     3241 tcagaaaact catcgtttgc tgcatgaggt ttagtttttg atcatgtccg gcgacggcgt
     3301 tattcacgcg tgtgacaccg tttgtcacct gctgcgccat cagtgccagc gaggcaaaaa
     3361 tggcaattgc caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg
     3421 gcctcccacg gcgctaaacc aggcgcgtcg tgactgaatc accgacgaaa agtcttcgtg
     3481 acggctgact tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt
     3541 tcgccagtac cagcggcttc ctgccatatc gctctcgccc tgatgcctga ccgcccgctg
     3601 ctcacgggtc atcaggtaga gcagcgcagt ctggttttca gctacccagc ccgctaccgt
     3661 tttttcctca agaacgtgct gattactcag cgcaccgctg gcaatctgta acaccgccgt
     3721 ggcagccagc gcaaaaatcg ccagcgcgag catcacttcc agcaaggtaa atccgcgctt
     3781 catggcatcc tctcatcaca ttgattgagg gataacgcgc catcgtgtgc cagctttacc
     3841 gcccaacatg cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca
     3901 aaaggcgaaa aacggatctg cggagtcttc tttttcgcct ctttttgcag ctccagttca
     3961 atatcgtgca gcgtcagtcg acgacgttgc agctccagcg catattcctt ctgccagata
     4021 tcacttccgg gttgtaacag catctgcacc tgtttcggca ccgtaacctg tgccgataac
     4081 cgggtcgagg aaacgggcga ccactgcccc tgacgacgct gcataaactg gtagccaggc
     4141 gggtcgatta gcacaccgag tgttttccct tcgatcactg ccctgtcctt aaactgcgca
     4201 aagcgagtca gaaaatcctg cgccgctttt ttcgcgggcg attcagaatc ggtcgcaaaa
     4261 gtctgtacca cgcccgcact ggcaaggccg ataagaaaaa tcaccagcat gatttccaga
     4321 agtgtgaatc cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata
     4381 tctgcgccag cgccttcccc gttttcctga ccatccgccc ccagggtata aacatcaaac
     4441 agcccttttt cacccggact gagatactga taatcattgc cccacggatc ctttggcagt
     4501 cgtttaatgt atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg
     4561 atcagcgcct caagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata
     4621 tccagtgcgt tttccagcgc cacgatatcg ctgatggctt tttgccgatc ggctttctct
     4681 ttgttgccca acaggttagg caccaccaga cttgccagga cgccaagaat aacaatcacc
     4741 accatcactt ccagcagggt aaaacctgcc cgtggttttt gtgtgcggga taacgaattc
     4801 atgtgataac tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag
     4861 gatggcgatg acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa
     4921 cagccccaac gccagcccca cctgggtatc aaattcccgt tcctggttaa cagcggcctg
     4981 ctcaagcatg gtttccagtt cgccgctctg ttcgccggag gcgatcatgt acagcatcat
     5041 cggggggaac aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg
     5101 atctgccgcc agcagtagtt gttgttcgac atagcgattt gccgacacag cggcagcggt
     5161 ctgaatacct tccagtagcg gaacactgct ggcggtgagg atgcttaagg tgcgggaaaa
     5221 acgcgccgta ttcagcccgc gcgcaacacg ccctgtcacg ggcagacgca gcacggtttt
     5281 atcccagcgc aggcgcatag taggattttt aagtagccgt tgccccagca ccagaagcgc
     5341 cagcaatccc gccagccagt aaacgccgct ggcctgtaac gcgtcgctca tggcaataag
     5401 cgtgcgggtt gaggcgggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg
     5461 caccactgcc gtcagcaaaa tagtgactac gcccgttgcc accaccagca gaaccagcgg
     5521 atagagcatg gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc
     5581 caggcgatta agcaccacgt cgagatgccc ggatttttct ccggcagcaa ccatcgaaca
     5641 aaacagagaa tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tgtaaccttc
     5701 ctgaatccgg ctgcgcagcg ccattccgag gctttttaca tgcagttttt cactttgctc
     5761 actgaccgcc tgtaagcagg tttccagcgg cattgctgcc tgtaccagcg ttgccagttg
     5821 gcgcgtgaac agcgcaagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg
     5881 caacatcccc cctgacgaag tattcatccg ggcttcaata tgcacgggga taagctcttt
     5941 accgcgcaac aactggcggg catgacgcgc ggaatccgcc tcaatcatac ctttggtttt
     6001 gcgaccatta cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc
     6061 cgcagaactt catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg
     6121 atacccgcag agtgttgtcg gacataacgt tccagctcca gctccccggc ctgacggtgg
     6181 atcaaatcac gcaatgtggc atccaccacg atcagctcat ggatggcagt ccgtccgcga
     6241 aaacctttgt gattacaggc gggacagccc tgtggatggt acagagtgac ggtacgggcg
     6301 tcggtaattc ccagcaggcg tttttcttcg tcagtggctg gtgcgggctg gcggcagtcg
     6361 ggacacagcg tgcgaaccag tcgctgcgcc atcacgcccg tcagactgga agagagcagg
     6421 aaaggctcca cgcccatatc ctgcaaacgt gtgatcgccc ccaccgctgt gttggtatgc
     6481 agcgtggaaa ggaccaggtg tccggtaagt gaagcctgga cggcgatttc tgcggtttcg
     6541 gtatcgcgaa tttcaccgac catcaccaca tccgggtcct gacgcaaaat cgcgcgcagc
     6601 ccacgggcaa aggtcatgcc gacgcgggtg ttaacctgcg tctgaccgat cccttcaatc
     6661 atgtattcga tgggatcttc aaccgtgaga atgttgcgcg aatggttgtt cagctcctgc
     6721 aatccggcgt acagcgtggt gcttttgccg gaacccgtcg gccccgtcac cagaaagatg
     6781 ccgtgcggtt tgtgtaacag ctggcgcaac tgcgcggtca gttgctggct aagccccaga
     6841 cgctccagcg tcaggcgggc ctggtttttg tccagcagtc gcagcaccac gcgctcgccc
     6901 caggcagaag gcatggtgga gacacgcacg tcaatcgccc ggccgcccag caacagcgca
     6961 atacgtccat cctgcggcac gcgcttttcg gcaatgtcca gccgcgccat caccttgata
     7021 cgcgacacca gcagcgaggc cagtttgcgc cccggacgca gcatttcatg taatgtgccg
     7081 tcaacacgaa aacggatcac cagactcttt tcaaacgtct caatgtggat atccgaagcg
     7141 ccttctttga ttgcctctgc cagcatggcg ttgatcagtt tgatgatcgg cgcatcgtcg
     7201 tcgctttcca gcagatcttc cgtttccggc agttcttccg cgagggtaaa aaagtcctcg
     7261 gcagaaccga gatcttccat cagttggcga gcctcggaag agtcccgctg ccagaccgca
     7321 ttcagccgct gttcaaattc ggcttcgtcg atttgccgca gcgtaaaggg cgcgttcagc
     7381 ccccgctgca gctcctgcaa aacagagagc gacaacggat gaacatggag gatctccagc
     7441 gacgcttcgc accatgccac caggctaaac cgacggctga aactgtaggg cagacgcacg
     7501 gtgttagcgg tggtttcctg tgctacaggc accattaacg cgttctcccg gcattaagga
     7561 acgcacgaac ttccggcggt agagcctgat tttgcgctgg cagtaccggc tgcgcggtgt
     7621 gcggcatcag gcttaaacct tgctcatcgc gatagatctg ctcggcgcgc atgtagttat
     7681 atttgcgctg cgacacgccg tctgccgcca taccgtcacg cagaatggtc gggcggataa
     7741 acaccatcag gttacgtttt tcttttttat ccgccgtcga tttaaacagg ttaccaatca
     7801 atgggatatc gcccagcagc ggcactttcg ccacgctttc tcccgcctga tcgtccatca
     7861 gaccaccaag cacaatcagc tcaccatcgt tagccagcac ggtggttttc agtttgcgct
     7921 caccaaacac cacgtcgagg ctggtctgtc cttccacctt cgagacttcc tgctcaatca
     7981 ccatctgtac cgcgttacct tcgttaatct gcggcgtgac tttcagcatg atgccgactt
     8041 ttttcctttc tactgtattg aaaggattgc tgttattgga gccaacggta gagccggtta
     8101 ataccggaac gtcctggccc accatgaaga aggcttcctg gttgtccagc gtagtgatgc
     8161 tcggcgtgga gagaacgttc gagctggagt cgtttttaac cgcctgcacc agtgccatcc
     8221 agtcgccttt caccacgcca accgccgtac cgctaaatcc agaaagaagc tgggcgagtg
     8281 tggagagatc gccgttggta tccggattaa tggtggtagc gccgttttca ctgatcaccg
     8341 tcgagccttt ctgcggtttc gcctgagaaa tggctgcccc cagcgtaccg atagggatct
     8401 gcgtaccatt agcaaactgc attaatccgg catctttcga cgcccactgc acgccgaagt
     8461 tgatattgct accttcggca acttccacga tcaacgcctc gacgtgcacc tgagcacggc
     8521 gaatatccag ttgttcaatc acgctttgca gcgactgcat gatgtcctgc ggtgcggtta
     8581 caatcagggc attactgtgt ttgctggcgg cgatggatac aatctcacgc ccgctaccga
     8641 ccgtgccttc cgcctcttct ttagccgccg tgagcgtgcc gctgacctgc ttcagcacat
     8701 cgaccagatc ttcggctttg ctgtatttga gatagaaaac ctggctgttg ccgctgcgtt
     8761 ccatttctga gtccagccga cggatcaggc ggcgcatttt gtcccgcgtg gccgggtcac
     8821 cactgacaat cacactgttg gtgcgttcgt cggcgacaat ttgagatttc agcgtcgctg
     8881 gctggttctc gccgctgttt ttcgtcaggc tttccagcac gcgggcgatt tccgaagccg
     8941 aggcgttatc cagcgggatc acctcttcgg tgcgattacc cgcgtgatcc acgcgctgga
     9001 tcacttccgt aaggcgctcc acaacggagg cgcgtccggt gagcataatc acgttggagg
     9061 gatcgtaatt aacaacgttg cctgagcctg cgctgtcgat catctggcgc agaatcggtg
     9121 ccaactcgcg taccgacaca ttacgtaccg gcactacttt ggtgaccatt tcatcgcccg
     9181 cgtagttgtc gctgccttca ccgaccagcg gcagcggctc gactttcgca gcgctggatt
     9241 tcaccacctt cagcacgtcg ttttccattg gtacgacggc atacccctgt acttccagca
     9301 ggttaaggaa tagctggtaa tactggcgtt cattgagcgg ggtcatggtg cgaatactca
     9361 ctttcccctg tacgcccggc cccataatga tggttttatt aaggttagcg ccgacggttt
     9421 cgatgaacga tttcaggtcg gtatctttaa aattagcggt gaaagtggct tcttccgccc
     9481 agaccggtga actgcataat gccgctgcca gcaccagcgg cagtaaacgc ttttttgttt
     9541 tgaggccagt tgtcttctta cgccagaccg acaacgtaat atcacgccaa aacacgatga
     9601 atgattctcc tggattaaat gcggttagcg cagcgcgatg gaaatgtcgt ggcgcgcacc
     9661 cttgcgtaaa accgtaagtt gaatggaatc cattgaaggt aactgccgca tcagagcaat
     9721 cattgctcgt ggatcagtga aatcctgctg atttagcgca atggcgatat cgccttcctt
     9781 gaaaccgctg gcatcgaaca gagaacgatc tgcccccggc ttcactgcat aaccgacaat
     9841 tccctcctta cgcacaggcg taagctggat atagttaaaa attttctgcg gatctttcgc
     9901 cagtgcctga cgcacggcag tcggtatctc aactggcgca ctgacagcag gttcagcaac
     9961 agcttgcttt gcttcgtcac tgacagcttt tttgttggtc gcggcaacgg tggaacgctc
    10021 ctcttcagcc aggctcaggc gctctatttt tccctgatag cgcagcatca catggtcgcg
    10081 attgatttcc tcaatcaccg cattgtgcga gccaagcgtt tcaccctgca aatagacctg
    10141 ctgtttaccg ccttcttcaa taaccgcgcc gggtctggca ccaaaggcaa tcccgcgcag
    10201 caccacatta agacgcgttt ctgccacagg cacaggttcg ggttgtttta ccggcgtggc
    10261 gacgggctga tatttgccaa accagttttg ctggctgatt aattgcacgt catttttatc
    10321 gaacgttttt gcatccgcac ggggcggttt attcgccgat ggggaaacag ccgtatattc
    10381 cgcagaaaag gagaaatagc gccagagtga atgcgccact tttgcagaaa taataagcag
    10441 catcagccag aacatcccgc gtacaatttt tcgcagatgc tctttgtctg tattaaaaga
    10501 ttgtctctgg ttaagagtgt gacgaatttt tgtcagccat tgaatgagat aaattcgtgc
    10561 gtcacgaaaa acaacccgcg ccaatttata tctacccgac gttatgcttt gactattcca
    10621 caggtggtac gatccagttt ccgctgatac tggttacctg tacgtgtatc attaatcgtt
    10681 atgctgtaat taattccctc ggtaatcatt aatttcaccg ttgggtcagc gcacatttgt
    10741 cgttgatagc tggttaaaaa ggcgtcaggc gtctgcgtgg tctgagtccc cgcttcgctg
    10801 ataatggtca tttttaccgt cgtgccgcta ctttgtgcca gcactaaggt atatcccgca
    10861 gatttaatcg gcaggttttg gcttatattt tgcgcctgtt ttttcgccag taaactggca
    10921 ttttcattat ggctggcaca gccacttaat aatcctgtaa cgctcaacaa tagcgatatt
    10981 aataccctcc ctggcatttg ttttatcgac atagtaaata atctctaagg ttattaataa
    11041 gagttaaaat gtcactttga taatgacgtg gttatcatta aaacaatgcc tgtagataaa
    11101 gtgttgctat accgcccaga cttaaacacg gtccaaaagg cagtgtggtt gatcctcttt
    11161 ttgtaataat ggcatatatc aggccgcagc atgatgcgat taaagcaaca ttgggcagtg
    11221 ataacggccc cacccagcca cctaacgcag cgaacaataa tacatcgccc atgcctaatg
    11281 cttctttacg cagaactatt ccggctatcc agcgcaggga gtaaaaagtg ataaacccca
    11341 ccaggacgcc ggtaactgcg tcttgtagcg ttaacggact ctgttgcgcc catgccgcaa
    11401 tcagtcctgt ccacaatacg ccctgagtaa aaacatcggg cagccattgg tgatcgaggt
    11461 cgatgatgct cgcggcaatc agccaggcgg ataatatcat caccgccagc gcccatccac
    11521 tttccggcca gaccagactc gccagcaaaa aagcgagtgc cgtcaataac tccaccagcg
    11581 gataacgctt gctgattttc gcctgacagt cgcggcagcg ccctttgagc atcaaccagg
    11641 agagcagcgg aatattgtca cgaacgcgga tggtctgctg acaatgtggg cagtgcgaac
    11701 gcggtagcgc aaggcttatt tttgactgcg cactcgacat ttcaccgtga aactccgcca
    11761 tttgttggcg cagcatgatg gggtaacgcc aaatcaccac attcaaaaaa ctgccgatga
    11821 tcaatcctcc gacggttgcc agtatgggca tcgccgcggg gtattgctga aaaacatcaa
    11881 aaagcatggt taaaggttgt ttgttgtaac ttgctggatg cgggcctgca agtgtatgcc
    11941 atacggcttt cctccaggcc cgataagctg gcgcaacccg gtgcgcctta tttcatgccg
    12001 gatgcggcgt gaacgcctta tccggcatac aggattactc gacagacatc ttatgctcgg
    12061 taaccttgtt aatgttttcc ggtccctgtt ccggtttcgg cagcttgagt gacgccagcg
    12121 tgttgtacgc cgactggctc acaccgccct cgaagctcat ctcgctcgcc cccggcagct
    12181 ggtaagcatt cgcacccgga ttccatttct taaagaactc cgaaagatcc gtctgggcga
    12241 cccaggaggc acacagcatc agcgtgtccg cagcgttacc gttggattca gcacagtaat
    12301 tcttgccacc aaaggtcttg tcaccaacgt catcgccgcg cgctttacgg tgcatcaact
    12361 ggaacaggtt ccagcctttc atcccttcac gatcgctgaa gaacttcggc aattcacctt
    12421 ctggatacca tttcttgata tcaaagtttt tctctgccca ttccttcagc tgcgcgtaca
    12481 tcagcagacg gtcacccgca ccgccgcgcg cccatgcctg accgttgctc tcctccagat
    12541 attccggtgc gacggtaata tcgtcagcga cacggttcat cttgccgaga tagcgatcct
    12601 gcatgtacag cgccagcacg ttgttcgcga cttcagttgc acccggtaca gtcagcggtg
    12661 tttctgcggc gttgtgccct acttcgtgcc agatcaacca gtcgttcagc ggcgtcgtcg
    12721 gcagcgtggt gctgttcgtc gagaagctgc tgttcattac cggataaccc gagtgcgcat
    12781 caccgatgga gatctgcaca tcgtttgtga aacgatgttt atgtcccgtc aatgctttat
    12841 aggtaaacat ccggtgctta ccgctcgttt catcacgacc gtagaagtca ttcatcgagc
    12901 tggcaaaggt gtccagatcg ttagcgaatt gctccagtcc gccagtgtaa ttgctggcat
    12961 tcaggttctt cttcggtgtg gtatagacga aagcgtctga ttccagctca cccagcggag
    13021 ccggtgagtt cagatcgttt ttccatgcgc cgtctttata gaacggtgct tttaccacgc
    13081 cagtaaaggt gaagctggca gattcattgg tagagctatt gcccttgata taaatcaggc
    13141 caccgtaagg caccttgaac ttcaccgtac cgctagcgtc cagagagtac gttttagtca
    13201 ctcttggcgg acggttcagc gcaacttcat gcttctcacg tccggtcagg tcgtcagcca
    13261 gcgccacggt gacggtcaca ggaacgttcg cattggactt aatggtgacc tctttctgag
    13321 ccggtgccca caggccagtt gactgcatgt tacctgcaaa ccatttggtc ggattcgagt
    13381 acaggctgat ggtttcagta acgttctgtc cctcttccga tactgctccc ggatacttct
    13441 cgacatcaac tttgatgttc agatcccacc aggaacggcc cagcatcagg cgtgtcagcg
    13501 gtttttccat atagttgagc gggtagctcg ggttcatcat gcccgctttg ctgctaccgt
    13561 caccgtagat catgttgtta tcgaccagcg attttttcag atctgcagaa cacttggtgc
    13621 cgcctgcata ggcatcattg gcgtagcagt tcaggaactc ggtgaacgtt ttaaagccca
    13681 gctcgtcatt tttgccttct tcataacgat agctcgtatc gttccacagc cagaccgaca
    13741 tgttctggta cagacgttcc agatcgacgc tgctcagacg ctcacctttg cgaccattgg
    13801 tccggaagta gagctcatgc tgatacagac gctgaatgtt ggtgcctaca tccgcagcct
    13861 gcagcatcgc tttcgcagtg tcagctccaa gatccagctg cgtatactgc ggaacataca
    13921 tgccaccggt taccggcaca cccgtgcctg ggcggtattc cagacagttg acctcatagt
    13981 gatagtcaga atccttacac acttccagcc ctgggaacgc gtccagaatt ctctgttttg
    14041 ccgcagccag tgactcaggc gttttgtggt cggcttcatc aataaaggcg aattgggtta
    14101 cctgtttacc ctcaacttct tcctgccagc tggcaacttc cagcttcggt ttgtcatcag
    14161 gtttgttttc ttgctgatat ttccagataa cttctttcgt ggtgtcatca atggtatacg
    14221 gtggtttacc atcgacagcc ggataacgct catagaccca aattggcgtt gaacgtcgtt
    14281 gacgaacacg gtcagggtag ccttgcggat cgttatttac taccgacttg ttaagcgcca
    14341 tcgacaaacc tgcggcatca agcagacgta caaagccaga tgcgctctct tccttaagat
    14401 tgctcatcac gttttccatg atcagcaccg atccaccttt gttcatatag gcgatcaaat
    14461 cggtcacatc ctgctgggtc agcttcggtt tgctggtatc tgcgcgcaga ggaatggagt
    14521 aaggatcgct accccattgt gtgacgtatt caaagccatt gaggatcaac agaggaactt
    14581 catctggatt cagattgcca tagctggtca ttggtttgac cgtgatgcca gtgaaatcct
    14641 tgtggaacgc aaacggtgcg ctatttccca gcacctgacc atgttttttg aaatataccg
    14701 tgtccaggtt agtacccacg gtcatactga attttgcatc cggcaaccag cgatcatttg
    14761 acagatagcg cagcacgttc tccatgaagt tcttcatgtc atccgggtcg ctgttgagcg
    14821 tacactgtcc gtctttatta acgcccccgt tccagctgta accgttcggg caacgcagga
    14881 tgctgttgta gtgtgggtta ccgataacca tcagcttgcc atcgcccact tgccccagcg
    14941 agataaacgg caggttgaag gtggcggttt cgcgtgtcac gttctctggt cgcacaatgg
    15001 aaggcgcttc agtaatgtac gccagctcat ttttatccca ggcgcgtttt tcgccaaacg
    15061 ccagccagta gtttttatca ttacgcgcca tcagaatcgg gaaggccgcg ttggagatat
    15121 tcaccaccgc ctgaccgcgc gcattacccg tgctgccata gaagttggta gagtcatgga
    15181 atacatggaa cttactgaca gatttgtagt tcgtatccac gccccacagc ttgttgataa
    15241 cgccctgaat ctggccgtca ttaacattgc gcgtcgtcag cgagaaccag cgagcctcgt
    15301 tacaaccatc ggttttcgca caaatcgcgg tatcgatctc tttggcctga cccgtcttaa
    15361 actgctcgat aaattcgtta ggcagattaa cgacttgctc accttccccc agcgtcgcac
    15421 cattggataa cgagagattg ataatctcgt taatcacgtt gggatattcg gcaaagacct
    15481 tgcgtacatc atccggaaca acacgggtat tattttgccc ggtcgtcgaa tagcgatgaa
    15541 taagctgatc gatattcgcc ccgcgaactt catcacccag ttcagtcagc gcaatggtcg
    15601 acttattgcc gcgcactgaa cccagttcaa aggtatcgat accaaaggag atggtttcgc
    15661 cccagctaaa agaaaattca ccattttccc ctgtcacgcc acggcctgaa ttggtgtagt
    15721 agttgacgcc agcaacacca tatccctggc tatccaccag gcgaccttct gagagaatga
    15781 tttccgaagg ttgatactga taaaactgtt ccgcgttagc cgacacgaag gaggcgttca
    15841 gatccggttt tgttcccgga gtcgtgacgg ggactaccgg tgaagtatga gtggatggcg
    15901 ctttatcagt cgcggcattg ttttccacct cttcattgac cagctttttg aactcttccg
    15961 gtgccagatc gatttgctta tacagcgagt cgaaacgttt actctcgatc accgaggaga
    16021 actccaggca cacctgttct gtattcgccg ggcaactgtt catggaggtg accagcgaga
    16081 gcgcattgct tttcttgttg tcggaacccg ccagttcttg cgcgtcctca agactaaacg
    16141 acactttttc aaccgcacgc aggctacgcg cagcttctga ctgggtgtcg aaggtagcaa
    16201 ttgtcgtgtt gttccctgcc acacaggtga ctttgtcgcc tggtgtaaag gtaaagccat
    16261 cgctggattc atcattacag gtgatatcac cagttacccg caggcttccg cccagggtca
    16321 gataacccgt tttcgtagga acaggttctg gctccggttc tggtgttggc tcaggatcag
    16381 gtgtcggttc cggcgtaggt tctgggtccg gcgttggctc aggcgtcggc tccgggtttg
    16441 gtgttggatc aggtttcact tccggcaaag accctgttcc agaatctaca ggcggcgtat
    16501 cggaggaaga tccggagcca ccgccatcac aaccggctaa cagggt
//
LOCUS       DAZKIV010000104        16411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZKIV010000104 DAZKIV010000000
VERSION     DAZKIV010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.104"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            332..1201
                     /gene="yjeJ"
                     /locus_tag="ACHOT6_003243"
     CDS             332..1201
                     /gene="yjeJ"
                     /locus_tag="ACHOT6_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFP9948412.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGTVVKT
                     REATPGKEIEAITHRILDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1251..1604)
                     /gene="yjeI"
                     /locus_tag="ACHOT6_003244"
     CDS             complement(1251..1604)
                     /gene="yjeI"
                     /locus_tag="ACHOT6_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFP9948413.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1742..3388)
                     /gene="groL"
                     /locus_tag="ACHOT6_003245"
     CDS             complement(1742..3388)
                     /gene="groL"
                     /locus_tag="ACHOT6_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFP9948414.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3432..3725)
                     /gene="groS"
                     /locus_tag="ACHOT6_003246"
     CDS             complement(3432..3725)
                     /gene="groS"
                     /locus_tag="ACHOT6_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFP9948415.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            4001..5257
                     /gene="yjeH"
                     /locus_tag="ACHOT6_003247"
     CDS             4001..5257
                     /gene="yjeH"
                     /locus_tag="ACHOT6_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFP9948416.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5273..5749)
                     /gene="fxsA"
                     /locus_tag="ACHOT6_003248"
     CDS             complement(5273..5749)
                     /gene="fxsA"
                     /locus_tag="ACHOT6_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFP9948417.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            6086..7522
                     /gene="aspA"
                     /locus_tag="ACHOT6_003249"
     CDS             6086..7522
                     /gene="aspA"
                     /locus_tag="ACHOT6_003249"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFP9948418.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7640..8941
                     /gene="dcuA"
                     /locus_tag="ACHOT6_003250"
     CDS             7640..8941
                     /gene="dcuA"
                     /locus_tag="ACHOT6_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFP9948419.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9057..9395
                     /gene="cutA"
                     /locus_tag="ACHOT6_003251"
     CDS             9057..9395
                     /gene="cutA"
                     /locus_tag="ACHOT6_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFP9948420.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9371..11068
                     /gene="dsbD"
                     /locus_tag="ACHOT6_003252"
     CDS             9371..11068
                     /gene="dsbD"
                     /locus_tag="ACHOT6_003252"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFP9948421.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11105..11680
                     /gene="yjdC"
                     /locus_tag="ACHOT6_003253"
     CDS             11105..11680
                     /gene="yjdC"
                     /locus_tag="ACHOT6_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9948422.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11787..11862
                     /locus_tag="ACHOT6_003254"
     tRNA            11787..11862
                     /locus_tag="ACHOT6_003254"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11820..11822,aa:Phe,seq:gaa)
     gene            12060..13322
                     /locus_tag="ACHOT6_003255"
     CDS             12060..13322
                     /locus_tag="ACHOT6_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9948423.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(13587..14498)
                     /locus_tag="ACHOT6_003256"
                     /pseudo
     CDS             complement(13587..14498)
                     /locus_tag="ACHOT6_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            14585..15010
                     /locus_tag="ACHOT6_003257"
     CDS             14585..15010
                     /locus_tag="ACHOT6_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9948424.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            15007..15357
                     /gene="tnpB"
                     /locus_tag="ACHOT6_003258"
     CDS             15007..15357
                     /gene="tnpB"
                     /locus_tag="ACHOT6_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9948425.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            15388..>16411
                     /locus_tag="ACHOT6_003259"
     CDS             15388..>16411
                     /locus_tag="ACHOT6_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HFP9948426.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRFAL
                     TRAEEKIEEDEREIEHLRAQIEKLSRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDAYCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLA
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLF"
BASE COUNT         4065 a         4178 c         4047 g         4121 t
ORIGIN      
        1 tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc gtttacgccg
       61 catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactga gaaatttaat
      121 ctttgcacgt tgcataacct ttaattattc atatgcattg ctgttgttac tttttatata
      181 gctaacacca ataattaacc tttcttttct gtttatatag agtgagcacg gctatttatg
      241 ttattttaac aactattact accaagccca tttatctggc ttataaatta aaattaatta
      301 tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg gtgttaatac
      361 cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta aagaacctcg
      421 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
      481 ggaaagtcgt ctgcatcaaa agcataaact ggatgctgct gcacgccttc agtatgagca
      541 agctcgcgat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      601 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      661 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      721 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      781 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      841 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      901 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      961 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
     1021 cactcaccga atacttgatt tcagcccacg actgaaaaaa ctcgcgggtg taccatgtca
     1081 ggtctatgtt cgcaccgtag cggcaaataa cgcccaaccg ctaacccagg atcaatgctt
     1141 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
     1201 atctcatctg ttttactacg aggctggaag tatccagcct ctggatcaca tcagttcgga
     1261 cacttatata cctggccgat aatctggcta tccgtcggga caaaactgga caacattccc
     1321 tgcgacgggc tgctgatgcc ataaatcacg ttaccgccca ttgcagccgc ctggttgcgc
     1381 agatcgtttg ctgcgccgcg catagaacca ccttcttctc cgtgttgccc ggaaagccag
     1441 ttgctttgct tacctgtcgc agtaccaatc agctggcact ctgcgcctgg ctgctcgtcc
     1501 acaatgcgta cactctgacc ggcagcactc aattcgttgc tggagctaca acccgccatc
     1561 agtagcgcgg caccgacaat ccctgctaag tattttacgt gcatgttatt ccccataatc
     1621 aatgagctgg acgccactcg cgtcgtccgt gtctgaatct tatactagaa agatgaccaa
     1681 aagaaaaacc cccagacatt tctgcccggg ggtttgttta tttctgcgag gtgcagggca
     1741 attacatcat gccgcccatg ccacccatgc cgcccatacc gccagcagcg cctaagtcag
     1801 ctgcatcgtt tttcggcagg tcggtaacca tgcactcggt ggtgatcatc aggccagcca
     1861 cagaagccgc gtactgcaga gcagaacggg ttactttggt tggatccagg atacccatgt
     1921 cgatcatgtt gccgtattct tcggttgctg cgttgtaacc gtagttgccg tcgccgcctt
     1981 taacggtgtt agcaacaaca gacggttctt cgccgcagtt caggacgatc tgacgcagcg
     2041 gagcttccat tgcacgcagt gcaactttga tacccacgtt ctggtcttcg ttctgaccac
     2101 gcaggtcagc cagtttagac gctacgcgga tcagcgcaac accaccacca gcaaccacgc
     2161 cttcttctac cgcagcacgg gtcgcgtgca gggcgtcttc aacgcgtgct tttttctctt
     2221 tcatttcaac ttcggtagca gcacctactt tgataactgc aacgccgcct gccagtttcg
     2281 ctacgcgttc ctgcagtttt tcacggtcgt agtcagaagt tgcttcttca atctgctgac
     2341 ggatctgagc aacacggccc tggattgcag cttcttcacc cacgccatcg atgatagtgg
     2401 tggtgtcttt gttgatcaca acacgtttag cctgacccag gtcttccagg gttgcttttt
     2461 ccagctccat accgatctct tcagagatta cggtaccgcc agtcagggtt gcgatatcct
     2521 gcagcatagc tttacgacga tcgccgaagc ccggagcttt aaccgcagca actttcacga
     2581 tgccacgcat ggtgttaaca accagagttg ccagcgcttc gccttcaaca tcttcagcga
     2641 tgatcagcag cggtttgcct gctttggcaa cagcttccag aaccggcagc atttcgcgga
     2701 tgttagagat tttcttgtca gccagcagga tgaacgggct ttccagttct actgcgccag
     2761 tttccggctt gttgatgaag taaggagaca ggtagccacg gtcgaactgc ataccttcaa
     2821 ccacgtccag ttcgtcctgc agaccggtac cgtcttcaac ggtgataacg ccttctttac
     2881 cgactttgtc catcgcttca gcgatcagtt tacctacggt ttcgtcggag ttagcggaga
     2941 tggtaccaac ctgagcaatc gctttagagt cagagcacgg tacggacagc gctttcagtt
     3001 cttcaactgc agcggtaacg gctttgtcga taccacgttt caggtccatc gggttcatgc
     3061 ccgcagcaac agctttcaga ccctcagtga tgatagcctg agccagtaca gttgcagtgg
     3121 tggtaccgtc gcctgcagcg tcgttcgctt tagaggcaac ttctttcacc atctgcgcac
     3181 ccatattttc gaacttgtct tccagttcga tttcacgagc aacggaaaca ccatctttgg
     3241 tgatggtcgg tgcaccgaaa gatttatcca gaactacgtt acggcctttc ggaccgaggg
     3301 taactttcac tgcatctgcc agtacgttta cgccgcgcag cattttcaca cgagcgtcgt
     3361 taccgaattt tacgtcttta gctgccatta tctttattcc ttaaattcgt atgttcagtg
     3421 tcgtgcgagg attacgcttc aacaattgcc agaatgtcgc tttcggacat gatcaacact
     3481 tcttcattgt cgatcttctc agatttcaca ccgtagccat cgttgaaaat aacgatgtcg
     3541 ccaactttca catccagcgg cttcacttcg ccattttcaa ggatacggcc attgccgaca
     3601 gccagcactt cgccgcgggt ggatttagcc gctgcagagc cggtcagaac gatgccgcca
     3661 gcagatttag tttcaacttc tttacgcttg acgatcacgc gatcatgcaa tggacgaata
     3721 ttcattgata actctccttt gagaaagtcc gtatctgtta tgggtgacgc cggagcttac
     3781 gtggtttccc ggctggtgac cagagaaatg gggatgagga ttcgcccctt caagggggaa
     3841 aaagaaaaaa aattctgatc agcacaaaat cgggtgaaaa ccctgattca cctcacattt
     3901 catcgcaatt tcttcatcgc cgtcataagc gaatctgatt gtgctaccat cgaaaatcta
     3961 cgcagttgcc caaaatttgg gcgcaatcgg atatcaaccc atgagtggac tcaaacaaga
     4021 actggggctg gcccagggca ttggcctgct atcgacgtca ttattaggca ccggcgtgtt
     4081 tgccgttcct gcgttagctg cacaggtagc aggcaataac agcctgtggg cgtggcccgt
     4141 tttgattatc ttagtgttcc cgattgcgat tgtgtttgcg attctgggtc gccactatcc
     4201 cagcgcaggc ggtgtcgcgc acttcgtcgg tatggcgttt ggttcgcggc ttgagcgagt
     4261 caccggctgg ttgtttttat cggtcattcc cgtgggtttg cctgccgcgc tacaaattgc
     4321 cgccgggttc ggccaggcaa tgtttggctg gcatagctgg caactgttgt tggcagaact
     4381 cggtacgctg gcgctggtgt ggtatatcgg tactcgcggt gccagttcca gtgctaatct
     4441 acaaaccgtt attgccggac ttatcgtcgc gctgattgtc gctatctggt gggcgggcga
     4501 tatcaaacct gcgaatatcc ccttcccggc acctggtaat atcgaactga ccgggttatt
     4561 tgctgcgtta tcagtgatgt tctggtgttt tgtcggtctg gaagcatttg cccatcttgc
     4621 ctcggaattt aaaaatccag agcgtgattt tcctcgcgca ttgatgattg gtctgctgct
     4681 ggcaggatta gtctactggg gctgtacggt agtcgtctta cacttcgacg cctatggtga
     4741 aaaaatggcg gcggcagcat cgcttccaaa aattgtagtg cagctgttcg gtgtaggagc
     4801 gttatggatt gcctgcgtga ttggctatct ggcctgcttt gccagtctca acatttatat
     4861 acagagcttc gcccgcctgg tctggtcgca ggcgcaacat aatcctgacc actacctggc
     4921 acgcctctct tctcgccata tcccgaataa tgccctcaat gcggtgctcg gctgctgtgt
     4981 ggtgagcacg ttggtgattc atgctttaga gatcaatctg gacgctctta ttatttatgc
     5041 caatggcatc tttattatga tttatctgtt atgcatgctg gcaggctgta aattattgca
     5101 aggacgttat cgactactgg cggtggttgg cgggctgtta tgcgttctgt tactggcaat
     5161 ggtcggctgg aaaagtctct atgcgctgat catgctggcg gggttatggc tgtttctgcc
     5221 aaaacgaaaa acgccggaaa atggcataac cacataatcc ggcgtttcga cattaatcct
     5281 ggcgatcgtc tttatgatca aggcggtcgc gctcatcatc ctttcgctgg tactcaccat
     5341 caaaagtatt accgccaccg gtcccggcgc taaaaccgcc gcctggcata cgagaaaagc
     5401 gcaaatgcgg catcaacttc actgtcagat gcttttgcac cggcggtaat aaaagcagaa
     5461 gaccgaggaa gtcggtgaaa aagcccggta ataaaagcag caaaccggca atgatcagcg
     5521 aaacactttt aatcatctcc gccgctgggt tttcacccgc cgccattttt tgctgcatca
     5581 gcacgaaatt cttaaagccc tggttacgta ccagtgacat cccgataact gacgtgaata
     5641 taaccagcac gagggtcagc aataccccca atacatgggc aacctgaata aagattgaaa
     5701 tctcaatata gacataaagg aaaatggcaa taaaaggtaa ccagcgcaaa ggtttctcct
     5761 gtaatagcag ccggtaaaac ccggctacct gaatgggttg cgaatcgcgt tcagcttata
     5821 ttgtggtcat tagcaaaatt tcaagatgtt tgcgcaacta tttttggtag taatcccaaa
     5881 gcggtgatct atttcacaaa ttaataatta aggggtaaaa accgacactt aaagtgatcc
     5941 agattacggt agaaatcctc aagcagcata tgatctcggg tattcggtcg atgcagggga
     6001 taatcgtcgg tcgaaaaaca ttcgaaacca catatattct gtgtgtttaa agcaaatcat
     6061 tggcagcttg aaaaagaagg ttcacatgtc aaacaacatt cgtatcgaag aagatctgtt
     6121 gggtaccagg gaagttccag ctgatgccta ctatggtgtt cacactctga gagcgattga
     6181 aaacttctat atcagcaaca acaaaatcag tgatattcct gaatttgttc gcggtatggt
     6241 aatggttaaa aaagccgcag ctatggcaaa caaagagctg caaaccattc ctaaaagtgt
     6301 agcgaatgcc atcattgccg catgtgatga agtcctgaac aacggaaaat gcatggatca
     6361 gttcccggta gacgtctacc agggcggcgc aggtacttcc gtaaacatga acaccaacga
     6421 agtgctggcc aatatcggtc tggaactgat gggtcaccag aaaggtgaat atcagtacct
     6481 gaacccgaac gaccatgtta acaaatgtca gtccactaac gacgcctacc cgaccggttt
     6541 ccgtatcgca gtttactctt ccctgattaa gctggtagat gcgattaacc aactgcgtga
     6601 aggctttgaa cgtaaagctg tcgaattcca ggacatcctg aaaatgggtc gtacccagct
     6661 gcaggacgca gtaccgatga ccctcggtca ggaattccgc gctttcagca tcctgctgaa
     6721 agaagaagtg aaaaatatcc aacgtaccgc tgaactgctg ctggaagtta accttggcgc
     6781 aacagcaatc ggtactggtc tgaacacgcc gaaagagtac tctccgctgg cagtgaaaaa
     6841 actggctgaa gtcactggct tcccatgcgt accggctgaa gacctgatcg aagcgacctc
     6901 tgactgcggc gcttatgtta tggttcacgg cgcgctgaaa cgcctggctg tgaagatgtc
     6961 caaaatctgt aacgacctgc gcttgctctc ttctggtcca cgtgccggcc tgaacgagat
     7021 caacctgccg gaactgcagg cgggctcttc catcatgcca gctaaagtaa acccggttgt
     7081 tccggaagtg gttaaccagg tatgcttcaa agtcatcggt aacgacacca ctgttaccat
     7141 ggcagcagaa gcaggtcagc tgcagttgaa cgttatggag ccggtcattg gccaggctat
     7201 gttcgaatcc gttcacattc tgaccaacgc ttgctacaac ctgctggaaa aatgcattaa
     7261 cggcatcact gctaacaaag aagtgtgcga aggttacgtt tacaactcta tcggtatcgt
     7321 tacttacctg aacccgttca tcggtcacca caacggtgac atcgtgggta aaatctgtgc
     7381 cgaaaccggt aagagtgtac gtgaagtcgt tctggaacgc ggtctgttga ctgaagcgga
     7441 acttgacgat attttctccg tacagaatct gatgcacccg gcttacaaag caaaacgcta
     7501 tactgatgaa agcgaacagt aatcgtacag ggtagtacaa ataaagaagg cacgtcagat
     7561 gacgtgcctt ttttcttgtg agcagtaact taaaaataac aacctaatat caacttgtta
     7621 aaaaacaagg aaggctaata tgctagttgt agaactcatc atagttttgc tggcgatctt
     7681 cttgggcgcc agattggggg gaataggtat tggttttgca ggcggattgg gggtgctggt
     7741 tcttgccgct attggcgtta aacccggtaa catcccgttc gatgtcattt ccattatcat
     7801 ggcggttatc gccgctattt ctgccatgca ggttgctggc ggtctggact atctggttca
     7861 tcagacagaa aagctgctgc gccgtaaccc gaaatacatc acgatcctcg caccgatcgt
     7921 gacctatttc ctgactatct ttgctggtac tggcaacata tctctggcga cactgccagt
     7981 tatcgctgaa gttgcgaagg aacaaggcgt caaaccttgc cgtccgctgt ctactgcagt
     8041 ggtatccgcg cagattgcga tcaccgcatc gccaatctca gcggcagtgg tttacatgtc
     8101 ttccgtgatg gaaggtcatg gcatcagcta cctccatctg ctctccgtgg tcatcccgtc
     8161 caccctgctg gcggttctgg tgatgtcctt tctggtcact atgctgttca actccaaact
     8221 ctctgacgat ccgatttatc gcaagcgtct ggaagagggc ctggttgaac tgcgcggtga
     8281 aaagcagatt gaaatcaaat ccggtgcaaa aacgtccgtc tggctgttcc tgctgggcgt
     8341 agttggcgtg gttatctatg caatcatcaa cagcccaagc atgggtctgg ttgaaaaacc
     8401 gctgatgaac accaccaacg caatcctgat catcatgctc agcgttgcaa ctctgaccac
     8461 cgttatctgt aaagtcgata ccgacaacat cctcaactcc agcaccttca aagcaggtat
     8521 gagcgcctgt atttgtatcc tgggtgttgc gtggctgggc gatactttcg tttccaacaa
     8581 catcgactgg atcaaagata ccgctggtga agtgattcag ggtcatccgt ggctgctggc
     8641 cgtcatcttc ttctttgctt ctgctctgct gtactctcag gctgcaaccg caaaagcact
     8701 gatgccgatg gctctggcac tgaacgtttc accgctgacc gctgttgctt ctttcgctgc
     8761 ggtgtctggt ctgttcattc tgccgaccta cccgacgctg gttgctgcgg tacagatgga
     8821 tgacacgggt acaacccgta tcggtaaatt cgtcttcaac catccgttct tcatcccggg
     8881 tactctgggt gttgccctgg ccgtttgctt cggcttcgtg ctgggtagct tcatgctgta
     8941 atgaccaatc gcggggcgtt cacgccccgc tttctttccc gccgactaac atccttcccc
     9001 cgtccgttgt atagtgacct ctctcttgcg gttccatctg ttcttgcgag gtgtttatgc
     9061 ttgatgaaaa aagttcgaat accgcgtctg tcgtggtgct atgtaccgca ccggatgaag
     9121 cgacagccca ggatttagcc gccaaagtgc tggcggaaaa actggcggcc tgcgcgacct
     9181 tgatccccgg cgctacctct ctctattact gggaaggtaa gctggagcaa gaatacgaag
     9241 tgcagatgat tttaaaaact accgtatctc accagcaggc actgctggaa tgcctgaagt
     9301 ctcatcatcc atatcaaacc ccggaacttc tggttttacc tgttacacac ggagacacag
     9361 attacctctc atggctcaac gcatctttac gctgatcctg ctactttgca gcacttccgt
     9421 ttttgccgga ttattcgacg cgccgggacg ttcacatttt gtccccgcgg atcaagcctt
     9481 tgcttttgat tttcagcaaa accaacatga cctgaatctg acctggcaga tcaaagacgg
     9541 ttactacctc taccgtaaac agatccgcat tacgccggaa cacgcgaaaa ttgccgacgt
     9601 gcagctgccg caaggcgtct ggcatgaaga tgagttttac ggcaaaagcg agatttaccg
     9661 cgatcggctg acgcttcccg taaccatcaa ccaggcgagt gcgggagcga cgttaactgt
     9721 cacctaccag ggctgtgctg atgccggttt ctgttatccg ccagaaacca aaaccgttcc
     9781 gttaagcgaa gtggtcgcca acaacgcagc gtcacagcct gtgtctgttc cgcagcaaga
     9841 gcagcacacc gcgcaattgc ccttttccgc gctctgggcg ttgttgatcg gtattggtat
     9901 cgcctttacg ccatgcgtgc tgccaatgta cccactgatt tctggcatcg tgctgggtgg
     9961 taaacagcgg ctctccactg ccagagcatt gttgctgacc tttatttatg tgcaggggat
    10021 ggcactgact tacacggcgc tgggtctggt agttgccgcc gcagggttac agttccaggc
    10081 ggcgctacag cacccatacg tgctcattgg cctcgccatc gtctttacct tgctggcgat
    10141 gtcaatgttt ggcttgttta ctctacaact cccctcttcg ctgcaaacac gtctcacgtt
    10201 gatgagcaat cgccaacagg gcggctcacc tggcggtgtg tttgttatgg gggcgattgc
    10261 cggactgatc tgttcaccat gcaccaccgc accgcttagc gcgattctgc tgtatatcgc
    10321 ccaaagcggg aacatgtggc tgggcggcgg cacgctttat ctctatgcgt tgggcatggg
    10381 cctgccgctg atgctaatta ccgtctttgg taaccgcttg ctgccgaaaa gcggcccgtg
    10441 gatggaacaa gtcaaaaccg cgtttggttt tgtgatcctc gcactgccgg tcttcctgct
    10501 ggagcgagtg attggtgatg tatggggatt acgcttgtgg tcggcgctgg gtgtcgcatt
    10561 ctttggctgg gcctttatca ccagcctaca ggctaaacgc ggctggatgc gcgtggtgca
    10621 aataatcctg ctggcagcgg cattggttag cgtgcgccca cttcaggatt gggcatttgg
    10681 tgcgacgcat accgcgcaaa ctcagacgca tctcaacttt acacaaatca aaacggtaga
    10741 tgagttaaat caggcgctcg ttgaagccaa aggcaaaccg gtgatgttag atctttatgc
    10801 cgactggtgc gtcgcctgta aagagtttga gaaatacacc ttcagcgacc cgcaggtgca
    10861 aaaagcgtta gcagacacgg tcttacttca ggccaacgtc acggccaacg acgcacaaga
    10921 tgtggcgctg ttaaagcatc ttaatgtcct tggcctaccg acaattctct tttttgacgg
    10981 acaaggccag gagcatccac aagcacgcgt cacgggcttt atggatgctg aaaccttcag
    11041 cgcacatttg cgcgatcgcc aaccgtgaac gacacttgca gtgggaaaga cggaggaaaa
    11101 tagcgtgcaa cgtgaagatg tactgggaga agccctgaaa ttattagaat tacaagggat
    11161 tgccaacacc acgctggaga tggttgctga acgtgtggat tatccactgg acgagctacg
    11221 ccgcttctgg ccagacaaag aggcaatcct ctacgatgcg ctgcgttatc ttagccaaca
    11281 gatagatgtc tggcgtcgtc agctgatgct ggacgaaacg caaaccgccg aacaaaagct
    11341 gctggcacgt tatcaggcgt tatcggagtg cgttaaaaac aaccgctatc cgggctgtct
    11401 atttatcgct gcctgtacgt tttatcccga tcctggccac cctattcatc aactggccga
    11461 tcagcaaaaa agcgcggcct acgatttcac ccacgaactg ttaaccacac tggaagttga
    11521 cgatccggcg atggtagcaa agcagatgga actggtgctg gaaggctgtt taagccgaat
    11581 gctggtgaat cgtagccagg cagatgtcga cactgcacat cggctggcgg aagatatcct
    11641 gcgcttcgcc cgctgccgcc agggtggtgc attgacctga cagaaacaca gaaaagaagc
    11701 gatttgccgc aatcttaagc agttgaatcg cttttactga aattaggttg acgagatgtg
    11761 cagattacgg tttaatgcgc cccgttgccc ggatagctca gtcggtagag caggggattg
    11821 aaaatccccg tgtccttggt tcgattccga gtccgggcac caaattcata tcaacggacc
    11881 tccacggagg tccgtttttc gtttcagaac accatgattt aagtgttctt cctccaaatc
    11941 aattctaccg aactcaacca gattctcccc acatcaacct cattgtgtgg gtataattgc
    12001 gggtataccc cagttcgaca gaatttgtac cctctttaac gcattcagaa ggctgaacta
    12061 tggcactgac tgacgcaaaa atccgggctg caaagcccac tgacaaggct tataaactca
    12121 ctgacggggc cggcatgttc ctgctggttc atcctaatgg ttcccgttac tggcgtctcc
    12181 gttatcgtat tctgggtaag gagaagactc tggcacttgg tgtgtatcca gaagtttctc
    12241 tctccgaagc tcgtacaaaa cgggatgagg cccgaaaact gatttcagag gggattgacc
    12301 cttgcgaaca gaaaagagtt aaaaaagttg tccctgattt acagctctct tttgaacata
    12361 ttgcacgacg ctggcatgcc agtaataaac aatgggcaca atcacacagc gataaagtac
    12421 tcaaaagcct cgagacacac gttttcccct ttatcggcaa ccgggatatc acaacactca
    12481 ataccccgga cctgcttatc cctgttcgtg ctgcagaagc aaaacaaatt tatgaaatcg
    12541 ccagtcgtct gcagcaaaga atatctgctg taatgcgtta cgccgtacag tctggcatca
    12601 tcagatataa cccggctctg gatatggctg gtgcattgac cacggtaaaa cgccagcatc
    12661 gccctgctct ggagctttca cgcctgcctg aacttctgtc gcgtattgac ggttataaag
    12721 gccagcctgt cacccgactg gcagttatgc tgaatttact ggtttttatt cgttccagtg
    12781 aactcagata tgcccgctgg tcggaaattg atattgacaa ttccatgtgg actattccag
    12841 ccgaacgcga acctctgcca ggcgtaaaat tctcacaccg gggatccaag atgcgaacac
    12901 cacatcttgt gccactcagc aaacaggttg tagccatact ggcagaactt cagacatggg
    12961 ctggtgaaaa tggtctgatc tttacgggag cacatgaccc acgtaaacca atcagtgaaa
    13021 acaccgtaaa taaagccctg agggttatgg ggtatgacac aacccaggat gtctgtggtc
    13081 atggattccg ggcgatggca tgtagtgcgt taatagaatc aggtttgtgg tcccgcgatg
    13141 cagttgaacg tcagatgagc catcaggaac gtaacggtgt acgtgctgct tacattcata
    13201 aagcagaaca tctggaagaa cggcgactga tgctacagtg gtgggcagat tttctggatg
    13261 cgaacagaga acaatgtatc agcccgtttg aatatgcaaa gattaacaat ccattaaaat
    13321 agtaatcatc ccgggctgcc agcccgggaa ttattttcaa tacctttttc tttgagatta
    13381 atgaataaac aaggcaattg acagaaccac ttccattacg tcatgtaatc aaaattttta
    13441 tagccgacat cagggatatc tgaacctcct ggtagcgggt aattcctctt caggtatttt
    13501 cgggtgctca actctggtta ttagcaaaat aatcgatata aacagtggaa aattcatcgt
    13561 acagaacatg gtgctcaaca ttactcttat tttcccttgc ccggaagaat cacaggctgt
    13621 gacttaaggc cgtccgcagg aactattttc tctccgttaa aaaccataac agtttcatta
    13681 tcttcattta tcagcgggtt gaatggcggt atattttcag tacgaatacc agtcagccac
    13741 tgaaaaatgc ctgcgaaatg acgggcactg atttttctgc tgacggactg gtgagatgtg
    13801 atgtcactgg cggtaataat caggggaaca ctgtagccac cctgcacatg gccatcatga
    13861 tgaacaggat tagcactgtc gctgaccgac atcccatggt cagaaaagta aagcatggca
    13921 aaatgacggg aataccggcg aaggatacca tcaagctgac cgagaaagtt atccagttta
    13981 ctgatgctgg cgaggtaaca ggcaaccttt cggggatact gctccaggta atgattcggc
    14041 caggagtgaa gccggtcaca cgggttcgga tgagacccca tcatgtgcag gaaaatcacc
    14101 ttcggagagg atttatccgc cagtgcacgt tctgtttcct gtaacaacaa catgtcatcc
    14161 gttttacggg aagcaaagct gcctttcttg aggaaaacgg tatgctccgc atcagatgca
    14221 ataacagaga tgcgtgtatc atgctccccc agttttccct gattggatat ccaccatgtg
    14281 ctgtatcctg cttttgctgc cagcgccacc acgttgttgc cggagtcagg gttctgctca
    14341 tagtcataaa tcagtgtccg gctcagggaa ggtacggtac tggctgctgc cgatgtatag
    14401 ccgtcaataa ataaaccggg agccatattc agccacggtg tggttggcac gggataacca
    14461 tataccgaca tataatccct gcgcacactc tcaccagtgg taagcgtaaa ctgaccgccg
    14521 tatgtagcca tcagacgaga attggtaact tagacgccca tctgatatag acggacatct
    14581 aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa aactattcga atgaattcaa
    14641 acttcgtatg gtggaactgg catcacaacc tggagcttgt gttgcacaga ttgcacgtga
    14701 aaatggcgtc aatgataatg ttattttcaa atggctcagg ctctggcaga acgaagggcg
    14761 tgtttcgcgg cgtcttccgg taacgacctc ttctgacact ggcgttgaat tattacctgt
    14821 agaaataacg ccggatgagc agaaagaacc tgtggcggcc attgcgccgt ctttatccac
    14881 ttccactcag accagagtca gtgccagttc ctgcaaggtg gaattccgtc acggtaacat
    14941 gacgctggaa aatccatcgc cagagctgct cacggtgttg atccgtgaac tgaccgggag
    15001 gggaagatga tctcactccc atcaggtacc cgtatctggc tcgttgccgg cgttaccgat
    15061 atgcgtaaat ccttcaacgg actgggagaa caggtacaac atgtgctgaa tgataatccc
    15121 ttctccggtc acctgtttat cttccgtggc cgacggggtg acaccgtcaa aattctttgg
    15181 gctgatgctg atggtctgtg cctgttcacc aaacgcctgg aggaaggcca gtttatctgg
    15241 cctgcggtac gtgacggcaa ggtatccatt acccgctcgc aactggcaat gctcctcgat
    15301 aagctggact ggcgtcagcc aaaaacatcc agccgtaact cactgacaat gttgtaaaaa
    15361 actcctgacg gcattataaa aacggtcatg agtcagaaat acctcattcg catcgcagag
    15421 ctggaaaggt tgctctctga gcaggctgaa gccctccgtc agaaagacca gcaactgagt
    15481 ctggttgaag agacggaagc cttcctgcgc tttgcactga cacgtgccga agaaaagatc
    15541 gaagaagatg aacgggaaat agaacatctg cgggctcaga tagaaaaact gagccggatg
    15601 ctgttcggta cccgttctga aaaactgcgt cgtgaagttg aactggctga ggctctgctg
    15661 aaacaacgtg aacaggacag cgatcgttac agtgggcggg aagacgatcc tcaggttccc
    15721 cgccagttgc gacagtcgcg ccatcgtcgt ccgttaccgg cacaccttcc ccgtgaaata
    15781 caccgcctgg agccagaaga aagctgttgt ccggagtgtg gcggtgagct ggattatctg
    15841 ggggaagtca gcgctgaaca gctggaactg gtgagcagtg ccctgaaagt gatccgcaca
    15901 gaacgggtaa aaaaagcctg tacaaaatgt gactgtattg ttgaagcacc ggcgccgtcc
    15961 cgcccgatag agcgtggtat cgcgggcccc ggattacttg cccgcgtgtt aacgggaaaa
    16021 tactgcgaac atctgccact gtatcgtcag agtgaaatct ttgcccgcca gggtgtcgaa
    16081 ctgagccggg ccttactctc caactgggtt gacgcgtact gccagttaat gacaccggtg
    16141 aatgatgccc tgtaccgtta tgtaatgaac acccgcaagg ttcacactga tgacacaccg
    16201 gtaaaggtac tggcaccggg tcagaaaaag gcgaaaacag ggcgtatctg gacgtatgtc
    16261 cgggatgatc gcaatgtggg ttcgtcatct cctccagcgg tctggttcgc gtactcgccg
    16321 aaccggcagg ggaaacaccc ggagcaacac ctccgcccct tccggggtat cctgcaggcg
    16381 gatgcgttca caggttacga caggttgttc a
//
LOCUS       DAZKIV010000105        16221 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZKIV010000105 DAZKIV010000000
VERSION     DAZKIV010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16221
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.105"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(48..424)
                     /gene="rnpB"
                     /locus_tag="ACHOT6_003260"
     ncRNA           complement(48..424)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHOT6_003260"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(457..1602)
                     /gene="garK"
                     /locus_tag="ACHOT6_003261"
     CDS             complement(457..1602)
                     /gene="garK"
                     /locus_tag="ACHOT6_003261"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFP9948427.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(1699..2589)
                     /gene="garR"
                     /locus_tag="ACHOT6_003262"
     CDS             complement(1699..2589)
                     /gene="garR"
                     /locus_tag="ACHOT6_003262"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFP9948428.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(2619..3389)
                     /gene="garL"
                     /locus_tag="ACHOT6_003263"
     CDS             complement(2619..3389)
                     /gene="garL"
                     /locus_tag="ACHOT6_003263"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFP9948429.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3405..4739)
                     /gene="garP"
                     /locus_tag="ACHOT6_003264"
     CDS             complement(3405..4739)
                     /gene="garP"
                     /locus_tag="ACHOT6_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFP9948430.1"
                     /translation="MILDTVDVKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            5114..6685
                     /gene="garD"
                     /locus_tag="ACHOT6_003265"
     CDS             5114..6685
                     /gene="garD"
                     /locus_tag="ACHOT6_003265"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFP9948431.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQGIPLENASIVSLQDEKHVGFQSMVEDILQIAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            6834..7169
                     /gene="prlF"
                     /locus_tag="ACHOT6_003266"
     CDS             6834..7169
                     /gene="prlF"
                     /locus_tag="ACHOT6_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFP9948432.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            7169..7633
                     /gene="yhaV"
                     /locus_tag="ACHOT6_003267"
     CDS             7169..7633
                     /gene="yhaV"
                     /locus_tag="ACHOT6_003267"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFP9948433.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLVVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(7688..8497)
                     /gene="agaR"
                     /locus_tag="ACHOT6_003268"
     CDS             complement(7688..8497)
                     /gene="agaR"
                     /locus_tag="ACHOT6_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFP9948434.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            8746..10026
                     /gene="kbaZ"
                     /locus_tag="ACHOT6_003269"
     CDS             8746..10026
                     /gene="kbaZ"
                     /locus_tag="ACHOT6_003269"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFP9948435.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFSILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            10049..10522
                     /gene="agaV"
                     /locus_tag="ACHOT6_003270"
     CDS             10049..10522
                     /gene="agaV"
                     /locus_tag="ACHOT6_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFP9948436.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            10533..11312
                     /gene="agaW"
                     /locus_tag="ACHOT6_003271"
     CDS             10533..11312
                     /gene="agaW"
                     /locus_tag="ACHOT6_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFP9948437.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            11302..12180
                     /gene="agaE"
                     /locus_tag="ACHOT6_003272"
     CDS             11302..12180
                     /gene="agaE"
                     /locus_tag="ACHOT6_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFP9948438.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            12198..12632
                     /gene="agaF"
                     /locus_tag="ACHOT6_003273"
     CDS             12198..12632
                     /gene="agaF"
                     /locus_tag="ACHOT6_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFP9948439.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            12629..13762
                     /gene="nagA"
                     /locus_tag="ACHOT6_003274"
     CDS             12629..13762
                     /gene="nagA"
                     /locus_tag="ACHOT6_003274"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFP9948440.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            14113..15267
                     /locus_tag="ACHOT6_003275"
     CDS             14113..15267
                     /locus_tag="ACHOT6_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFP9948441.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            15280..16140
                     /gene="kbaY"
                     /locus_tag="ACHOT6_003276"
     CDS             15280..16140
                     /gene="kbaY"
                     /locus_tag="ACHOT6_003276"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFP9948442.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
BASE COUNT         3885 a         4328 c         4176 g         3832 t
ORIGIN      
        1 gctcctccaa aagcaaaaac ccgccgaagc gggtttttac gtaaatcagg tgaaactgac
       61 cgataagccg ggttctgtcg tggacagtca ttcatctagg ccagcaatcg ctcactggct
      121 caagcagcct acccgggttc agtacgggcc gtaccttatg aacccctatt tggccttgct
      181 ccgggtggag tttaccgtgc cacggactgt taccagccgc gcggtgcgct cttaccgcac
      241 cctttcaccc ttacctgatc ccgcttgcgc gggccatcgg cggtttgctc tctgttgcac
      301 tggtcgtggg tttccccccc aggcgttacc tggcaccctg ccctatggag cccggacttt
      361 cctcccctcc gcccgtctcc cccgaagagg acgacgacga agcggcgact gtctggtcag
      421 cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac cccgcgttgc gcattccaat
      481 cgccagtgtc gcggcgatat tacgtgaagc acggcagata ttgtcataag ccccgcggaa
      541 tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag accgcatcga tgccatgctg
      601 atgtacaacg ccaacatcat cggtcaggct acccgcaatg ccaatcaccg gtttatggta
      661 cttcttcgcc acgtttgcaa cgccaatcgg taccttgccg tgaatactct ggctgtcaat
      721 acgcccttca ccggtgatca ccagcgtaca atcgtgaata tgttcctcca gattcagcgc
      781 cgtagtgacg atttcaatac cacttttcag ttccgcacca agaaacgcca tcagcgccgc
      841 acccataccg cccgcagctc ctgcgccggg gacatctttc acatcaacat gcagcgcttt
      901 tttaatgaca tcggcatagt gagagaggtt attgtccagc tcaacaatca tcgcttcact
      961 ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta tcgcccacca gcggattggt
     1021 gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc
     1081 aatatcattc agagtattaa gactaccgcc gccaaaacca atttcattgc cgttggcgtc
     1141 gcataatttc gcccccagcg cctgtaccat gcctgcgccg ccatcatttg tagcgctacc
     1201 gccaatgccg ataataatgt ttgtcgcgcc gctctccagc gcctgcagga ttaactcgcc
     1261 tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc gcaggtacca gctccagccc
     1321 actggccgcc gccatttcaa taaacgcggt tttgccatcg ccggagatcc cccaactggc
     1381 attcactttc tcgcccagcg gccctgtaac ccaggcgtga cgttcagaac cctgggtggc
     1441 tgcaatcatc gcttccaccg ttccttcgcc accgtcggca accggaatag aaacgtactg
     1501 tgcatcagga aaaatttccc gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga
     1561 taaactttct ttataagagt ctggggcgat tacgattttc atacctatgc ctgttaccac
     1621 atgacgccgg agggcgtttc tcttattcgg cctggattcc aggcccggat tgcaatacgc
     1681 catccgggca cgacgtcatt aacgagtaac ttcgactttc gccagttttt cgtagtagca
     1741 cgccagggcg ctatgatccg ccgttcctaa accatctgct cgcagtgcct gcatcatctc
     1801 cataaccgca gctgtgagcg gcagttgcgc gccgacgccg tgggaagtat ccagcgcatt
     1861 tgccagatcc ttaatatgca gatcaatacg gaagcccggc ttgaagttgc ggtccatcac
     1921 catcggcgct ttggcatcca gcacggtact gcccgccagt ccaccgcgaa ttgcctgata
     1981 caccaggtcc gggttaacgc ccgctttggt tgccagcgtt agcgcttctg acatcgcggc
     2041 aatattcagc gccacaatga cctgatttgc cagtttggtg acgttacctg caccgatttc
     2101 cccggtatgc accacggaac ccgccatcgc tttcatcaaa tcatagtatt tgtcgaaaat
     2161 agccttgtcg ccgcccacca tcactgacag cgtaccgtcg atggctttcg gttcaccgcc
     2221 gctcaccgga gcatccagca tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg
     2281 gcttgccagc ggtgcgatag aactcatatc gatcaatacc gtacctggct tcgcgccttc
     2341 aatgatgcca ttctcaccca gcgccacctc tttcacatga ggggagtttg gcagcatggt
     2401 tatgatgacg tcgcactgtt cagcgatcgc tttagccgta gacgctgttt ctgcacctgc
     2461 agcaatcacg tcagcaatag cttctgggtt acggtcagca accaccagcg agtaacctgc
     2521 tttcagaagg tttttactca ttggtttacc cataatcccc aggccaataa aaccaacttt
     2581 catagtcata tcaatcatct ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc
     2641 agtttctgag tggcagagcg gaagacgcca agatcgctgc cgacagccac aaacgtcgcg
     2701 ccccattcca gataacgacg cgcatcggct tcgaccggcg cgagaatacc gctgggtttg
     2761 ccgtgcgcac tggcacggtt aaaaatgtgc tgaattgctt tttgtacatc cgggtgcgag
     2821 gcattgccga gatggcctaa tgccgcagcc agatcgctgg ggccgacgaa gataccgtct
     2881 acaccttcgg tagcggcaat ggcatcgacg ttatctacgc cctgctgact ttctatctgg
     2941 accagaatag tgatgttctt gttcgactga gcgaaataat ccgccacggt gccaaacata
     3001 ttggcgcggt gagaaacgga gacgccgcga atgccttccg gtgggtaacg ggttgatgcc
     3061 accgcctgct ctgcttcctc ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg
     3121 atatccagaa gacgcttaat aattaccggc tcgttggtcg gcactcgcac tactggcgcg
     3181 ctggcgctgc ctttcagggc cattaactgc ggaataaacg tggaaatatc gtttggcgca
     3241 tgttcgccat ccagcaccag ccagtcaaac ccagccaaac caagaacttc agtgctaatc
     3301 gggttagaga gtgctgacca gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg
     3361 aatttattcg ggaaaacatc gttattcatc gcttatacct ttgcttattt ctgcaattcc
     3421 atacgtttaa tgtcgccaac tacgaagagg tagcagacca tcgccatcag cgctgaacat
     3481 cccacgaaaa ccagtgctgc attgaaggag tgcagttcac ttaccaggta gccaatcacc
     3541 agtggagtga caatggaggc aacattgcca aagacgttaa agacgccgcc gcagaggcca
     3601 acaatctctt tcggcgcggt gtcagaaatc accggccagc ccagagcacc aaatcctttg
     3661 ccaaaaaaag ccagcgccat cagcatgacc accagcgtgg tgttgttggt gtagttacat
     3721 aagatgatgg tggaagccag caacattccc agcacaatcg gtagcttacg tgccagggtc
     3781 agggataaac cgcgtttgat cagataatcc gagaagacac ctcccagcac gccgcccgca
     3841 aaaccacaca gtgccggaat cgaggcgacc agacccactt tcagaatcga catgcctttt
     3901 tcctgcacca gataaatcgg gaaccaggtg aggaagaacc aggtgatggt gttgataaaa
     3961 tattgtccga aaaatacgcc caacatcatg cggttagaga gcaattgctt gatgtaatgc
     4021 agtttgggac cgcttgctgc cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg
     4081 ccattttcag agataaactt cagctcttcc gcagacatac gtgggtgatc tgtcgggtta
     4141 tgaatcaact tgatccacag cgccgtcagc acaaaaccaa tcacccccat aacggtgaag
     4201 acgtgctccc agccccaggc gaaagtcagc cagccaagca gcggcgaaaa gagcgccagc
     4261 gagaaatatt gcgccgagtt aaagatggcg gaggcagtac cacgttcttt cgtcgggaac
     4321 caggcggcga caatacgggc gttcgccggg aatgatggcg cttccgagaa ccccagcata
     4381 aagcgcataa agaacatgga gatccctgcc caggccagcg ggaacatatc aacaaagcct
     4441 tgcaggaagg tgaacagcga ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca
     4501 aacttatcaa gcagccagcc gccgggaatt tgcatcagca ggtaggccca gccaaaagcg
     4561 gagaagatgt aacccatcga aaccgcactt aactgcaact cttttgccac ttcggtacca
     4621 gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg taacaataaa aataatcagt
     4681 aatattaaat agcgggtatg cacgcctttc ttttttacgt caacggtgtc cagaatcatt
     4741 ttatttacct cgggtactta tgctgatttt tattattatg gtgaaggtgt tatttatgag
     4801 ttttatttat gccgttacga caatgaactc gggaattagt ataagcagcg cgagaataat
     4861 aatcattgtg caaatgctaa tttaattaat accatttaaa tattattttg agcatatgca
     4921 cataaggttg cgcgctaaag cacagatttg cgctttacct tgccgggcgg cactgcaatc
     4981 cctgaaatga ttgacattga tcacatttct gcgtttaaac tcctgacatt cttatttcac
     5041 ccaatgaagt catttatttt taaatgagac caggtcctca ttttaataac ccctggctgg
     5101 agaatattgc acaatggcca acatcgaaat cagacaagaa acgccaactg cgttttatat
     5161 aaaagttcac gacacagata atgtggcaat tattgttaat gataatggcc tgaaagcagg
     5221 aacgcgtttt ccggatgggc tggaattaat tgaacatatt ccccaggggc ataaagtcgc
     5281 attgctggac attccggcta atggtgaaat tattcgttat ggcgaagtga ttggttacgc
     5341 cgtgcgcgca atcccacgcg gaagctggat cgacgaatca atggttgtac taccggaagc
     5401 gccgccgtta cacacgctgc cactggcaac caaagtcccg gaacccttac cgccgctgga
     5461 aggttacacc tttgaaggct atcgcaatgc cgatggcagc gtgggcacca aaaacctgct
     5521 cggcatcacc accagcgtcc actgtgtggc aggcgtggtg gactacgtag taaaaatcat
     5581 tgaacgcgat ctgctgccga aatacccgaa cgtcgatggc gtggtggggc tgaatcatct
     5641 gtacggttgt ggcgtggcga ttaacgctcc ggcggcggtg gtgcccattc ggaccattca
     5701 caacatttcg cttaacccaa actttggcgg cgaggtgatg gtgattggcc tgggttgcga
     5761 gaaattacag ccagaacgcc tgctggtcgg cacggatgat gtacaaggta ttccgctgga
     5821 gaacgccagt attgtcagct tgcaggatga aaagcatgtc ggttttcagt ccatggtcga
     5881 ggatattttg cagatcgccg aacgccatct acaaaaactg aatcaacggc agcgagaaac
     5941 ctgcccggct tcagaactgg tcgttggtat gcagtgcggt ggcagcgatg cgttttctgg
     6001 tgtaacggca aacccggcgg ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac
     6061 ggtgatgttt tcagaagtaa cggaagtgcg tgacgcgatc catctgctga caccacgcgc
     6121 agtgaacgaa gaggtcggca aacggctgct ggaggagatg gagtggtacg ataactatct
     6181 caatatggga aaaaccgacc gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct
     6241 ggcaaacgtg gtagagaagg cactcggctc cattgctaaa tcgggtaaaa gcgcaattgt
     6301 tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc
     6361 agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg ggtatcacag tgcaagtgtt
     6421 tacgaccggt cgtggtacgc cgtacggcct gatggcggta cccgtcatta aaatggcaac
     6481 ccgcaccgag ctggcgaacc gctggtttga tttaatggat attaatgcgg gcaccatcgc
     6541 taccggcgaa gaaactattg aagaggtggg ctggaagttg ttccacttta ttctcgacgt
     6601 cgccagcggg aagaagaaaa ccttctcgga tcaatgggga ctgcataacc agctggcggt
     6661 gtttaacccg gcaccggtga cctgatttct tttggaacgt ccctcgcaaa acatggcctt
     6721 agtgccatgt ttttattgtt taaagccccc acgtccatta ataatgcatt tgcattacct
     6781 tagttcaagc ttataatttg agcagaaaac aggctgtaaa aggacagtga atcatgcccg
     6841 ctaatgctcg ctctcacgct gtactgacca ctgaatcaaa ggtcacgata cgcggacaaa
     6901 caactatccc cgcgccagtg cgtgaggcct taaaactgaa gccaggccag gacagcattc
     6961 attacgaaat tctgcctggt gggcaagtct ttatgtgccg actgggagat gaacaggagg
     7021 atcatactat gaatgcattt ttgcgttttc tggatgcaga tatccagaac aacccgcaaa
     7081 aaactcgtcc attcaacatt caacaaggaa agaaacttgt cgctggcatg gacgtcaaca
     7141 ttgatgatga gattggcgac gacgaataat ggattttcca caaagggtta atggttgggc
     7201 gctatatgct catccctgtt ttcaggaaac ctacgacgct ttagttgccg aagtcgagac
     7261 attaaaggga aaagatcctg aaaattatca gagaaaagcc gccacaaagt tattggtggt
     7321 agtccataaa gtgattgagg agcatatcac ggtcaatcca tcatcaccgg cattccgtca
     7381 tggcaagtcg ttaggctctg ggaaaaataa agactggtca cgggtaaaat ttggtgctgg
     7441 tcgttatcgt ctcttctttc gttatagtga aaaagagaaa gtcatcattc tgggatggat
     7501 gaacgatgaa aacactctgc gcacctacgg taaaaaaaca gatgcctata ccgtattcag
     7561 caaaatgtta aaaagaggac atcctcctgc cgactgggaa agcctcaccc aagagacaga
     7621 agaaaaccat tgatggtgtt cacaatgccc gccgaacgta ttcgtgttga ccagcgggca
     7681 ccctttctca ctccccgacc agaatcactt caaccccagc ctttcgcagt ccttccagac
     7741 tatccgcagg aatgccttca tcaacaatga tcatgtcgat acgttgagta tcaatgatct
     7801 tatgtaaact ggaacgattg aatttactgg aatcggtgac cacgatgatc cgttccgcaa
     7861 cttcgcacat ccgacggttt aaacgggctt catcttcatt atgtgtgctg acgccgcgct
     7921 ccagatcgat cgcatctaca ccaagaaaca gcatatcgaa gtggtaattt tgcagcgatt
     7981 gctcagcctg atcgccgtaa aaagattgcg actgacggcg caaatgcccg ccggtcatca
     8041 gcagctcaac gccttccgct tccagcaacg cattagccac gttcataccg ttggtcatcg
     8101 caattacgtc agtgtgctta cgcatcagac gagcaatctc aaaagtggta gtcccggaat
     8161 cgaggatcac ccgatgacca ggctgaatca actcaacggc agctttcgca acgctgcgtt
     8221 tcatcgcggt gttcagcgcg cttttatctt ccactgatgg ctcgactgac ggcgtcgtgc
     8281 tatcgcagat caacgcgcca ccataggcac gcacagcgat cccctgcttt tccagaaacg
     8341 ccagatcatt gcggatcgtc acagtagata cgccatacaa tgccgacaga tcgttaacct
     8401 gcacactccc ttgctgtcgc agacgctgaa tgatctgttc tcgtcgctcg ctggtgccag
     8461 tcactcgctt ctcacctgaa gcgtcggtat tactcatagt aagtcctttc gtaaaacttt
     8521 cgtttcattt cgttttgcct attaacgcct ttctattaag caaatgcaag cccaccttgc
     8581 ccattgacgc aagctactct cgtttcagtg actttcatta tgtttctttt gtgaatcaga
     8641 tcagaaaact attatctttc gttttatttt tatctcacca tgacgcagta tcaactgaaa
     8701 caaaacgaaa gattaatatc gcagtaatct gaactggaga ggaaagtgaa acatctgaca
     8761 gaaatggtga gacagcacaa agcgggcaaa acaaatggaa tttatgccgt ttgttccgca
     8821 catccgctgg tgctggaggc tgcaatccgc tacgccagtg caaaccaaac gccgttactg
     8881 attgaagcaa cctccaatca ggtagaccag ttcggcggtt ataccggaat gacgcccgcc
     8941 gattttcgcg gctttgtttg tcagctcgcc gactcgttga atttcccgca ggatgcgttg
     9001 attctgggtg gtgaccatct ggggccaaac cgctggcaaa acctgccggc cgctcaggca
     9061 atggccaatg ccgatgattt gattaaaagc tacgttgcgg caggattcaa aaaaatccac
     9121 cttgattgca gcatgtcctg tcaggacgat ccgattccct taactgatga catcgtggct
     9181 gaacgcgccg cccgtctggc gaaagtggcg gaagaaacct gtcttgaaca ctttggcgaa
     9241 gccgatctgg agtatgtcat tggtaccgaa gtgccggtac ctggcggcgc gcatgaaacc
     9301 ttaagcgagc tggcggtcac cacgccggat gccgcccgcg ccacgctgga agcccatcgt
     9361 cacgcctttg aaaagcaagg tttgaatgcc atctggccac gcatcattgc tctggtggtt
     9421 caacccggcg tcgaattcga tcacaccaac gttattgatt atcagcccgc caaagcgagc
     9481 gccttaagcc agatggtcga aaactacgaa acgctgattt tcgaagcgca ctctaccgat
     9541 tatcaaacgc cgcaatcgct gcgccagctg gtgattgacc acttttccat tctgaaagtt
     9601 ggcccggcgc tgaccttcgc cctgcgtgaa gccctttttt ctctggcggc gattgaagaa
     9661 gaactggtac cagcgaaagc ctgttccggt ctgcgtcagg tgctggaaaa cgtgatgctc
     9721 gaccgcccgg aatactggca aagccactac cacggtgacg gcaacgcgcg tcgtctggcg
     9781 cgtggttata gctactcgga tcgcgtgcgc tattactggc cggacagcca gattgatgac
     9841 gctttcgccc atctggttcg taatctggcg gattcaccga ttccgctgcc gctcatcagc
     9901 cagtatctgc cgctgcaata cgtgaaagtt cgctccggcg agctgcaacc gacgccacgg
     9961 gaactcatta tcaaccatat tcaggacatc ctggcgcagt actacacagc ctgtgaaggc
    10021 caataagcaa aacaaagagg aacacgctat gccaaatatt gttttaagcc ggattgatga
    10081 acgcttgatt cacggtcagg tcggcgttca gtgggtcgga tttgcggggg caaatctggt
    10141 gctggtagcc aacgatgagg ttgccgaaga tccggtacaa caaaacctga tggaaatggt
    10201 actggcagaa gggatcgccg tacgtttctg gacgctgcaa aaagttatcg acaacattca
    10261 tcgcgccgcc gatcgacaga aaatcctgct ggtttgtaaa acacccgccg atttcctgac
    10321 gctggtgaaa ggtggcgttc cggtgaatcg cattaacgtt ggcaatatgc actacgccaa
    10381 tggcaaacaa caaatcgcca aaacggtttc tgtggatgcg ggcgatatcg cagcatttaa
    10441 cgacctgaaa gccgctgggg tggaatgctt cgttcagggc gtcccgacag agcctgctgt
    10501 ggacctcttt aaattacttt gagggattca tcatggaaat cagcctgttg caggcatttg
    10561 cgttgggcat tatcgccttt atcgctggcc tggatatgtt taacggctta acccatatgc
    10621 accgcccggt ggtcctcggc ccgttggtcg ggctggtact tggcgatctg cataccggaa
    10681 ttttaaccgg cggtacgctg gaactggtgt ggatggggct ggccccgctg gcgggcgcac
    10741 agccgcctaa cgtgattatc ggtactatcg tcggcacggc gtttgccatt actactggcg
    10801 tgaaacccga tgtcgcagtc ggtgtcgccg tacctttcgc tgtcgcagta cagatgggga
    10861 ttaccttcct gttctcggtg atgtccggcg tgatgtctcg ctgcgaccgg atggcggaga
    10921 acgccgatac tcgcggcatt gaacgcgtga actatctggc actgctggca ctcggcactt
    10981 tctattttct ctgcgctttc ctgcctatct acttcggcgc ggaacatgca aaaaccatca
    11041 ttgatgtcct gccgcaacga ttaatcgacg gcctcggcgt cgcgggcggc atcatgccag
    11101 caatcggttt tgccgtgctg ctgaaaatca tgatgaaaaa cgtctacatc ccctacttca
    11161 tcctgggctt tgttgccgca gcctggctca agttaccggt gctggctatt gctgccgctg
    11221 cgctggcgat ggcgctgatc gacctgctgc gtaaatctcc tgaaccgact caacctgcgg
    11281 cacagaaaga ggaatttgaa gatggcatct aatcaaacca ccctgccgaa cgtcagtgaa
    11341 aacgaagaaa cattgctgac tggcgtcaat gaaaacgtgt atgaagatca gagcattggc
    11401 gcggagctga cgaaaaaaga tatcaaccgc gtcgcctggc gttccatgct gttacaagcg
    11461 tcttttaact acgaacgtat gcaggcttct ggctggttgt acggtctgct gcctgctctg
    11521 aaaaagatcc acactaataa acgcgacctg gcgcgcgcca tgaaggggca tatgggtttc
    11581 ttcaataccc atccgtttct ggtgacattt gttatcggca ttatccttgc gatggagcgt
    11641 tctaagcagg acgttaacag tattcagagc accaaaattg ccgtcggtgc gccgctcggc
    11701 gggattggtg atgcgatgtt ctggctgacg ctactgccga tttgtggcgg gataggcgca
    11761 agccttgcgc tacaaggctc tattcttggc gcagtcgtat ttattgtgct gttcaacgtg
    11821 gtacacctgg ggctgcgttt tggtctggcg cattatgctt accgcatggg cgtggcggcg
    11881 attccactca ttaaagcgaa taccaaaaaa gtcggccatg ctgcgtctat cgttgggatg
    11941 acggtaatcg gcgcgctggt ggcaacctat gttcgtttaa gcaccacgct ggaaatcaca
    12001 gcgggcgacg cagtggttaa gttacagact gatgttattg acaaactgat gcctgccttc
    12061 ttaccgctgg tctacacctt gaccatgttc tggctggtac gccgcggctg gagtccgctg
    12121 cgcctgattg cagttaccgt ggttctcggc atcgtcggta aattctgcca tttcctttaa
    12181 atacaatgag gtattagatg ttaagtatta ttttgacagg gcatggcggg tttgccagtg
    12241 gcatggaaaa agcaatgaag caaattctcg gtgagcaatc gcagtttatc gccatcgatt
    12301 ttccggaaac ctcaagcacc gcgctgctta catcgcagct tgaagaggcc atcgcccaac
    12361 tggattgtga agacggcatt gtttttctga cggatttgct gggcggcacg ccgtttcgcg
    12421 tagcttcaac gctggcgatg caaaaaccgg gctgtgaagt gatcaccggc accaatttgc
    12481 aactgctact ggagatggtg ctggagcgcg aagggttaag cggtgaagaa ttccgtgtac
    12541 aggcgctgga gtgtggacat cgtgggctga ccagtctggt ggacgagtta ggccgctgcc
    12601 atgaagaatg tccggtcgag gaagggatat gacacacgtt ctgcgcgcca gaaggctgct
    12661 gactgaagag ggatggctcg atgaccatca gttgcgtatt gctgacggtg tcatcgcagc
    12721 aatcgaaccg attccagtgg gcgtgactga acgcgatgcg gaactgctct gccccgctta
    12781 catcgacacc catgtacacg gtggtgcggg cgttgatgta atggatgacg cgccggatgt
    12841 actcgacaag ctggcaatgc acaaggcacg cgaaggtgtc ggcagttggt tgccgactac
    12901 cgtaaccgcg ccgcttaaca ccattcatgc ggcgctgaaa cgtattgctc aacgttgcca
    12961 acgcggcgga cctggtgcgc aagtgctggg gagttatctc gaaggaccgt acttcacgcc
    13021 gcagaataaa ggcgcgcatc cgccggaatt gtttcgcgag cttgaaattg ccgagctgga
    13081 tcaattgatt gccgtttctc agcacacctt acgcgtggta gcgctggcac cggaaaaaga
    13141 gggggcattg caggccatcc gccatcttaa acagcaaaat gtacgagtga tgctggggca
    13201 tagcgcggcg acctggcaac aaactcgcgc cgcgtttgat gctggtgccg acggcctggt
    13261 gcattgctat aacgggatga caggtttaca tcaccgcgaa ccgggaatgg ttggcgcggg
    13321 attaacggac aagcgcgcct ggctggaact gatagccgat ggtcatcatg tgcatccggc
    13381 ggcgatgtcg ctgtgttgtt gctgtgcaaa agagagaatc gtgctgatca ccgacgcgat
    13441 gcaggcagcc gggatgccgg atggtcgcta tacgttatgt ggcgaagaag tgcagatgca
    13501 cggtggcgtt gtccgtaccg cgtccggtgg gctggcgggc agtacgctgt ctgttgatgc
    13561 ggcagtgcgc aacatggtcg agttgacggg cgtaacgcct gcggaagcca ttcatatggc
    13621 atcgctgcat ccggcgcgaa tgctgggtgt tgatggtgtt ctgggatcgc ttaaaccggg
    13681 caaacgcgcc agcatcgttg cgctggatag cgggctgcat gtgcaacaaa tctggattca
    13741 gagtcaatta gcttcgtttt gatattttgc tcctttattg ggccttcact tcccccgtaa
    13801 ggcctttctt tttctttcgt tttgatctgt gcagcggtgt cggatgcgac gctaacgcgt
    13861 cttatgcgac ctacggtacg ccgcatccag caatcggtga acatagcgac gtcattacgc
    13921 aattctttaa tcttcccttg tcttttcttt caatgatcac agatttcgtt ttatttcctt
    13981 tttctccatt gaactttctg tttcttttct atagatttta atcaacgaaa gacatcacca
    14041 agtgaaatga aacgaaaggc aagtgaaagc gacaacgccc gacgtcaagt tcatcagact
    14101 aaggattgag ttatgccaga aaattacacc cctgctgccg ccgcaaccgg tacatggact
    14161 gaagaagaga tccgccatca gcctcgcgca tggatccgtt cactcaccaa catcgacgcg
    14221 ctacgttctg cgctcaataa cttccttgaa ccgttactgc gcaaagagaa tctgcggatc
    14281 atcctgaccg gagccggaac ctcggcattt atcggtgaca tcatcgcgcc gtggctcgcc
    14341 agccataccg gtaaaaactt cagcgccgta ccgaccaccg atctggtcac caatccgatg
    14401 gactacctga acccagctca tccgctgctg ttgatctcct tcggtcgatc cggcaacagc
    14461 ccggaaagcg tcgctgccgt ggaactggca aatcaatttg taccagaatg ctatcacctg
    14521 ccgatcacct gcaacgaagc gggcgctctt taccaaaacg cgatcaacag cgataacgcg
    14581 tttgccctgc tgatgcccgc agaaacgcac gatcgcggct ttgcgatgac cagcagcatt
    14641 accaccatga tggccagctg cctcgcggtt ttcgcacctg agacgatcaa cagccaaacc
    14701 ttccgcgacg tggcggatcg ttgccaggcg atcctgacct cactgggcga tttcagcgaa
    14761 ggtgtgtttg gttacgcacc gtggaaacgg atcgtttatc tcggcagcgg tggcttacag
    14821 ggcgcagcac gcgagtcggc gctgaaagtg ctggaactga ctgcgggtaa actggcggcc
    14881 ttctatgatt ccccgaccgg attccgtcat ggcccgaaat cactggtcga taacgaaacg
    14941 ctggtggtgg tatttgtctc aagccaccct tacacccgtc agtatgatct tgatctgctg
    15001 gctgaactcc gccgtgacaa ccaggcaatg cgcgtaatcg ccatcgccgc ggaaagcacc
    15061 gacatcgtcg ctgccggtcc acatattatc ctgccgccgt cacgtcactt tatcgacgtt
    15121 gagcaggcat tttgcttcct gatgtacgcc cagacgtttg cactgatgca gtcgctgcac
    15181 atgggcaata cgccggatac cccatcagcc agcggcaccg ttaaccgcgt ggtgcaaggc
    15241 gtaatcattc atccgtggca ggcataagag gatcgcatta tgagcattat ctccactaaa
    15301 tatctgttac aggacgccca ggccaatggc tacgcggtgc ctgcttttaa cattcataac
    15361 gccgagacga tccaggcgat cctcgaagtg tgcagtgaaa tgcgatcgcc ggtgatcctc
    15421 gccggaacgc cggggacctt taaacatatc gcgctggaag agatctacgc cctgtgcagc
    15481 gcctattcca caacctacaa catgccactg gcgctgcatc tcgaccacca cgaatcgctg
    15541 gatgatattc gccgtaaagt ccacgcaggt gtgcgcagcg caatgatcga cggcagccac
    15601 ttcccgtttg ccgagaacgt gaagctggtg aaatcggttg ttgacttctg ccactcacag
    15661 gattgcagcg tggaagcaga actgggccgc ctgggcggtg ttgaagatga catgagcgtt
    15721 gacgccgaaa gtgcattcct gaccgatcca caagaagcta aacgctttgt tgaactgact
    15781 ggcgtcgaca gcctggcggt agcgattggt acggcgcacg gcttatacag caaaacgccg
    15841 aagattgatt tccagcggct ggcggaaatt cgtgaagtgg tggatgttcc tctggtgcta
    15901 catggtgcca gcgatgttcc ggatgaattt gtccgtcgca ctattgaact tggcgtcaca
    15961 aaagtgaacg ttgccacaga attaaaaata gccttcgctg gcgcggttaa agcctggttt
    16021 gcggaaaatc cgcagggtaa tgatcctcgt tattatatgc gcgtcggaat ggatgcgatg
    16081 aaagaagttg tcagaaataa aattaatgtc tgtggttcag cgaatcgaat ttcagcataa
    16141 tcattagttt tttatgattt atcccaatgt acttcccgat taatatcggg gagtgcctta
    16201 atggaaaagg agatgactaa a
//
LOCUS       DAZKIV010000106        15665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZKIV010000106 DAZKIV010000000
VERSION     DAZKIV010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.106"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..1488
                     /locus_tag="ACHOT6_003277"
     CDS             124..1488
                     /locus_tag="ACHOT6_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9948443.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="ACHOT6_003278"
     CDS             complement(1616..2107)
                     /gene="yajQ"
                     /locus_tag="ACHOT6_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFP9948444.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2275..3186
                     /gene="panE"
                     /locus_tag="ACHOT6_003279"
     CDS             2275..3186
                     /gene="panE"
                     /locus_tag="ACHOT6_003279"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFP9948445.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3149..3739
                     /gene="yajL"
                     /locus_tag="ACHOT6_003280"
     CDS             3149..3739
                     /gene="yajL"
                     /locus_tag="ACHOT6_003280"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFP9948446.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="ACHOT6_003281"
     CDS             complement(3793..5241)
                     /gene="thiI"
                     /locus_tag="ACHOT6_003281"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFP9948447.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5447..5689
                     /gene="xseB"
                     /locus_tag="ACHOT6_003282"
     CDS             5447..5689
                     /gene="xseB"
                     /locus_tag="ACHOT6_003282"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFP9948448.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5689..6588
                     /gene="ispA"
                     /locus_tag="ACHOT6_003283"
     CDS             5689..6588
                     /gene="ispA"
                     /locus_tag="ACHOT6_003283"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFP9948449.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6613..8475
                     /gene="dxs"
                     /locus_tag="ACHOT6_003284"
     CDS             6613..8475
                     /gene="dxs"
                     /locus_tag="ACHOT6_003284"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFP9948450.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8530..9504
                     /gene="yajO"
                     /locus_tag="ACHOT6_003285"
     CDS             8530..9504
                     /gene="yajO"
                     /locus_tag="ACHOT6_003285"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFP9948451.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9558..10076)
                     /gene="pgpA"
                     /locus_tag="ACHOT6_003286"
     CDS             complement(9558..10076)
                     /gene="pgpA"
                     /locus_tag="ACHOT6_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFP9948452.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10054..11031)
                     /gene="thiL"
                     /locus_tag="ACHOT6_003287"
     CDS             complement(10054..11031)
                     /gene="thiL"
                     /locus_tag="ACHOT6_003287"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFP9948453.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11109..11528)
                     /gene="nusB"
                     /locus_tag="ACHOT6_003288"
     CDS             complement(11109..11528)
                     /gene="nusB"
                     /locus_tag="ACHOT6_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFP9948454.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11548..12018)
                     /gene="ribE"
                     /locus_tag="ACHOT6_003289"
     CDS             complement(11548..12018)
                     /gene="ribE"
                     /locus_tag="ACHOT6_003289"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFP9948455.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12107..13210)
                     /gene="ribD"
                     /locus_tag="ACHOT6_003290"
     CDS             complement(12107..13210)
                     /gene="ribD"
                     /locus_tag="ACHOT6_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFP9948456.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKDATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13214..13663)
                     /gene="nrdR"
                     /locus_tag="ACHOT6_003291"
     CDS             complement(13214..13663)
                     /gene="nrdR"
                     /locus_tag="ACHOT6_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFP9948457.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13814..14353
                     /locus_tag="ACHOT6_003292"
     CDS             13814..14353
                     /locus_tag="ACHOT6_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFP9948458.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14652..15536
                     /gene="tsx"
                     /locus_tag="ACHOT6_003293"
     CDS             14652..15536
                     /gene="tsx"
                     /locus_tag="ACHOT6_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFP9948459.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
BASE COUNT         3778 a         4153 c         4066 g         3668 t
ORIGIN      
        1 gaatgcaaaa aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa
       61 cactctgttt acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg
      121 gtaatgaacg attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc
      181 gtattctcgt tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc
      241 atggctctgc aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg
      301 actcaggccg tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca
      361 ttaattgtcg gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac
      421 tccatctggg gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc
      481 gttatggcgc tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt
      541 atcggcgtga gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact
      601 cacaaacttg ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt
      661 gcgttgacca tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga
      721 atggtgaaag gcagtttcag taaagtgctg gcggaaccgc ggctgctgaa actcaacttt
      781 ggcattatgt gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta
      841 gctgatgcgg ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc
      901 gcctttggct cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa
      961 gtctttgtct tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcacaa
     1021 acgcagttct ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg
     1081 gaagcccttc tgccttcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg
     1141 atgggtgttt actccaccag ccagtttctt ggcgtggcga ttggcggttc actgggcggc
     1201 tggattgacg gcatgtttga cggtcagggc gtatttctcg ctggcgcaat gctggccgca
     1261 gtgtggctgg cagtcgccag taccatgaaa gaaccggcgt atgtcagcag tttgcgcatt
     1321 gaaatcccgg cgaacattgc cgcaaacgag gcgttaaaag tgcgtttgct agaaactgaa
     1381 ggcatcaaag aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc
     1441 aaagtgacga atcgctttga tgttgaacag gcaattcgcc aggcataaaa gcgaggcgaa
     1501 attgcctgct gcgctacgct catcaggcct actaagctcc tgatttacaa acctttgtag
     1561 gccgaaccag gcgttcatgc cgcatctgac atgaacaaag cgcaggttgt cgcgattaat
     1621 cgcggaagtt tttgaactgg aacggctgac ccagatcgcc accgcgcacc atcgccatga
     1681 cagcctgcaa atcatcacga gatttgccag tcacgcggat ctcatcgccc tgaatttgcg
     1741 cctgcacttt cagtttgctg tctttgatca tcttgacgat ttttttctgg gtcgcgctct
     1801 caatgccctg cttcagtttc gcctccacaa accaagtttt accgctatga acgatatttt
     1861 ccggtacatc cagtgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat
     1921 ccagcaactg attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt
     1981 cgttcagctc aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc
     2041 tcgcgttatc gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag
     2101 atggcatctt ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat
     2161 aaccgacagt cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac
     2221 aattaaggga tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa
     2281 attaccgtat tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag
     2341 ggtcatgaag ttcagggctg gctgcgcgta ccgcaacctt attgtagtgt gaatctggtt
     2401 gagacagatg gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttctcgcc
     2461 accagcgatc tgctcctggt gacgctgaaa gcatggcagg tttccgatgc cgtcaaaagc
     2521 ctcgcgtcca cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc
     2581 atcgaagagt tgcaaaatat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc
     2641 cgccgcgacg gcaatgtcat tattcatgtg gcaaacggta tcacgcatat tggtccggca
     2701 cggcaacagg atggcgatta cagttatctg gcggatattt tacaaaccgt gttgcctgac
     2761 gttgcctggc ataacaatat tcgcgccgag ctgtggcgca agctggcagt caactgcgtg
     2821 attaatccac tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa
     2881 gaaattatgc agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact
     2941 tcagcagaag atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc
     3001 tcgtcgatgt tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt
     3061 tttctcttac gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt
     3121 gaaatggtaa aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc
     3181 tggtagtgaa gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa
     3241 agtcaccact gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt
     3301 gaagctgctg gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt
     3361 actgcctggt ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac
     3421 cgttaaacag ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac
     3481 cgtgctggta ccgcacgata tcttcccgat tggcaatatg accggcttcc cgacgctgaa
     3541 agacaaaatt cccgccgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa
     3601 attgctgacc agccaggggc cgggtacggc tattgatttt ggtctgaaaa ttatcgacct
     3661 gctggttggg cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat
     3721 ttataattat tacgagtagt gtcggatgcg gcaaacgtcg catctgacca gatgcgacgt
     3781 aaaaaccaca aattatgggc gatacacctt cacattgtta aagccctgct cgcgcagata
     3841 gagcgcctgc agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg
     3901 gtcgagatcg ccaaatttgg tgctcagttt atagaacggc agagaaacca cgtcgatacc
     3961 ttcaaccttc agcggcttat cttcctgctc atcgacagaa cggatatcga ggatcacgtc
     4021 gttcgggcca aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc
     4081 gatttcgcgg atatcaacgt tattcgcttc ctcaaccact ttatcgagaa tactgaagtc
     4141 gaacttctct tcttccgctt caatcttcga tttaaccgct ttcaccgtcg ggcttttgga
     4201 gatcacgcca caatattctg gcatcgtgcg agcaaagtct tcagtgccaa tctggcgggc
     4261 caggttgatg atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtgtcaga
     4321 gacgttatca atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc
     4381 gccggtgacc agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat
     4441 catacgtttg aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccaac
     4501 gaccggttcg aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca
     4561 cagataatgc gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa
     4621 acagtaatgc acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa
     4681 accaccggaa atgagcgaca gcacgtcttc ctgggtgccg atcgggaaac cgccaatacc
     4741 ttcgtagcgg cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac
     4801 ttccggattg gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac
     4861 gtaacgttcc acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca
     4921 gaaggttttg ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg
     4981 catgtcggta aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt
     5041 cagggcgtcg cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc
     5101 ccagtggcgg acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc
     5161 ggtaaggatt tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa
     5221 caatttaatg ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta
     5281 tagggaaaaa tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta
     5341 acatcgcttt gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt
     5401 ccgttaccat gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa
     5461 tgaggctccc gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct
     5521 ggaaagtggc gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct
     5581 ggcacgtcag gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc
     5641 tgacaatgaa gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg
     5701 cagcaactcg aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca
     5761 ctgccctttc agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt
     5821 aagcgcctgc gaccttttct ggtttatgcc accggtcata tgttcggcgt tagcacaaac
     5881 acgctggacg cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat
     5941 gatttaccgg cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag
     6001 tttggcgaag caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt
     6061 ctaagcgatg ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa
     6121 ctggcgagcg ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg
     6181 gaaggcaaac acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca
     6241 ttgattcgcg ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct
     6301 ctgccagtac tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac
     6361 atcctggatg tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa
     6421 cttggtaaaa gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcacgg
     6481 gatctgatcg acgatgcccg tcagtcgctg aaacaactgg ctgaacagtc actcgatacc
     6541 tcggcactgg aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt
     6601 aataggcccc tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc
     6661 acccaggagt tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc
     6721 cgctatttac tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg
     6781 gtcgaactga ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg
     6841 gatgtggggc atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc
     6901 accatccgtc agaaaggcgg tctgcatccg ttcccgtggc gcggcgaaag cgaatatgac
     6961 gtattaagcg tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct
     7021 gccgagaaag aaggcaaaaa tcgccgcacc gtctgtgtca ttggcgatgg cgcgattacc
     7081 gctggcatgg cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg
     7141 gtcctcaacg acaatgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa taaccatctg
     7201 gcacagctgc tttccggtaa gctttactct tcgctgcgcg aaggcgggaa aaaagttttc
     7261 tctggcgttc cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg
     7321 gtagtgcctg gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt
     7381 cacgatgtgc tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag
     7441 ttcctgcata tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc
     7501 actttccacg ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc
     7561 ggtttgccga gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac
     7621 aacaagctga tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca
     7681 cgtaaattcc cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt
     7741 gccgccggtc tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcctg
     7801 caacgcgcct atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc
     7861 gccatcgacc gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac
     7921 ctctcttacc tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa
     7981 tgtcgccaga tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac
     8041 ccgcgcggta acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa
     8101 ggcattgtga agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca
     8161 gacgcggcga aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa
     8221 ccgcttgatg aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta
     8281 gaagaaaacg ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat
     8341 cgtaaaccag tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact
     8401 caggaagaaa tgcgcgccga actcggcctc gatgccgccg gtatggaagc caaaatcaag
     8461 gcctggctgg cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg
     8521 agtggcagca tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc
     8581 ctcggctgta tgacctttgg cgagccagat cgtggtaatc acgcatggac actgccggaa
     8641 gagagtagcc gtcccatcat taaacgcgcg ctggaaggcg gcataaattt ctttgatacc
     8701 gccaatagct attccgatgg cagcagcgaa gagatcgttg gtcgcgcact gcgggatttc
     8761 gcccgtcgtg aagacgtggt cgttgcgacg aaagtgttcc atcgcgttgg tgatttaccg
     8821 gaaggattat cccgtgcaca aattttgcgc tctatcgacg acagcctgcg ccgtctcggc
     8881 atggattatg tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagag
     8941 acgctggaag ccctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca
     9001 tccatgcacg cttcgcagtt tgctcaggcc ctgaagctac aaaaacagca cggctgggcg
     9061 cagtttatca gtatgcagga tcactacaat ctgatttatc gcgaagaaga gcgcgagatg
     9121 ctgcctctgt gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg
     9181 cgactgacgc gtccgtgggg agaaactacc gcacgactgg tgtctgatga ggtggggaga
     9241 aatctctata aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc
     9301 agtgaagaac tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg
     9361 ggcattgccg caccgattat cggtacatcg cgggaagaac agcttgatga gctgctgaac
     9421 gcggtggata tcactttgaa gccagaacag attgccgaac tggaaacgcc gtataaaccg
     9481 catgcggtcg taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat
     9541 caggcatcgg tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac
     9601 aggatgcctg cggaaatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca
     9661 tgtacattgc gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa
     9721 atcacaaacc cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc
     9781 cacataccaa taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttc
     9841 gccgtttgat gacaaagata gacgccgata cagatcccca acatcaccac cagcgagtag
     9901 agctgccagg gtaaaaaggt catcagatac cagaacggaa tcgctgccag cgatcccatc
     9961 gtgccaggaa cgattgggct taatccactt ccgaatccga cagcaagcag atgccacgga
    10021 ttactcatct tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc
    10081 tttccagtca aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc
    10141 ggtcatttgc ccgatacagg taaacggtac gcccaggtgt ccgagagcca catccagcgc
    10201 gccacggttc agttccggca cagtgaaaca caactcgtaa tcttcaccgc cagagagcgc
    10261 ccagcgcagc gcctgttccg gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa
    10321 tgccaggtca atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat
    10381 cagaccgtca gagagatcga tagctgaatt tgccagatcg cgcagtgcct gtccctgtaa
    10441 aatacgcggc gatggacgga gatgacgttt gatcaagtag tccgcatctt tagcatcggc
    10501 aacctgcaaa cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt
    10561 cacatagatc cagtcacccg gtttcgctcc agagcgcgtt aaggctcgtc ccatcggaac
    10621 aaagccgtgg atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag
    10681 ttgcatatcg taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc
    10741 ttcgtctacg tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc
    10801 tgccagatcg cttaggttca ccgccagtgc tttatacgcc agatcagcag gatcgatatc
    10861 atggaggaag tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc
    10921 ggggatattg agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact
    10981 tcttacacgg tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc
    11041 ctcagcaggt aaaaaagaaa aggccggcga tcgcggaacg gtcttccgtg aatctaccgg
    11101 cctggatatc actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg
    11161 acgaacttat ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg
    11221 gccactttgt atggcacatc gctacgttta gacagttcgt acagcgcaat gcgcagtact
    11281 gctttttcta cctgtcccag ttcttccagc aggcgggaca ggtatggctt catcagtccg
    11341 tcgaggtatg cggtattagt cgccaccccg gccagcagct cacggaagta caggacgtca
    11401 acgtctttta catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg
    11461 gacaactgcc aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca
    11521 ggtttcacgg atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat
    11581 ttcaagcgcg gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc
    11641 gatcgcttgt tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc
    11701 ctgggcaaca tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt
    11761 gccaccacga ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag
    11821 tgcacccgcc gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc
    11881 atcttttacc tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt
    11941 gataaagttg ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt
    12001 agcttcaata atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga
    12061 ttttagcata atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta
    12121 aatgcaggca aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt
    12181 cggctaattt ctcaagccct ggcagcgtgc ataatccgcg ggcgtcgctg cctaatagtt
    12241 taggtgcgat atagacaatc agctcatcga ctaaccccgc ctgcagcaat gcgccagcga
    12301 gcgttggccc cgcttccacc cagatgctgt taatttgctg tttacccagt tgcatcatca
    12361 gtacaaccag atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg
    12421 gccactcact agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa
    12481 tgcgatgttc cggcgtcacg cgattttggc tatcaatcac aatacgtact ggctggcgga
    12541 gattttgttg cgggtagagc gcctgagttt gttcatccag ttcagaccaa cgcactgtta
    12601 aggctggatc atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgtgcgc
    12661 gctgtcgttg tacgtcgcgc cgcgcctgag gcgaagtgat ccactggctt tcgccgctcg
    12721 ccatcgccgt gcggccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg
    12781 tgcgcatccg cttgagaaag cctttattca attgctcggc ttcactcatc atcagcccgt
    12841 ggctgacgtc aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt
    12901 taggatcttg catcgaggca accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc
    12961 acggtggcgt acgaccatga tggctacagg gttcgagtgt gacataggcg gtcgcatctt
    13021 tggctttttc acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgcacgtt
    13081 ggtggtaacc ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt
    13141 tgggatgcgt ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact
    13201 cgtcctgcac ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt
    13261 cgaaactgcg gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt
    13321 gctccatcac cagattgcca atcatcttgc tcggcacttc gcgctcaccg gtggcgcgca
    13381 gctgcgattt aatatgattg attgccattt cgacgtcatc ggaactcacc ggacgttttt
    13441 ccagcgcccg cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt
    13501 tgctttttac aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat
    13561 tacacaccag acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt
    13621 caattacctt agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg
    13681 tggttaacag aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg
    13741 agataaagga tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag
    13801 aaagggacga cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag
    13861 ccttagcgcc tgtgtgcagc agagcgaagt gcgacagatg aaacacagcg tcagcacgct
    13921 gaaccaggag atgacgcaac tcaataaaga aacggtcaaa atcactcagc aaaacaggct
    13981 gaatgcaaaa tccagcagtg gggtttacct tctgcctgga gcgaaaacac cggcaagact
    14041 ggaaagccag atcggtactt tacgtatgtc gctggtgaat attacgcctg atgcagatgg
    14101 caccaccctg acgctacgta ttcagggcga gtcgaatgat cccttgcccg cattcagcgg
    14161 aactgttgaa tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca
    14221 gaatcaattg attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt
    14281 acagttaaag ggtatttctg tagaacagtt agacttcgta cgtatccacg atattcaacc
    14341 tgttatgcag taaacgtatt tcggggcgat tttgtgcgtc ccgcaacatc tttccccgtc
    14401 attttgttac tccgcttaca tcacccagat tgatagtaaa agtttgcaac aagggtgaaa
    14461 gtcagtacaa tccctgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat
    14521 agaactgtga aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta
    14581 tacgaaatat ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca
    14641 gtggcataca tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt
    14701 cttttactgt caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc
    14761 agagcgttaa cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata
    14821 cctaccttga gtacgaggca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg
    14881 atgcgccggt attcttcggc ggtaactccg atgctaaagg tatctggaac cacggttctc
    14941 cgctgtttat ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta
    15001 gcttcggtcc gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta
    15061 ataaagatgg tcgccagagc acctggtaca tgggtctggg taccgacatc gatactggtc
    15121 tgccgatgag cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag
    15181 cgaacgaaaa cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg
    15241 atctgtgggg cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt
    15301 taggggatga cagcggtaac gcaatcaacg gtattaagac ccgtaccaat aactctatcg
    15361 cttccagcca tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt
    15421 actggcacga cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact
    15481 tcaacgttcg ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgattat
    15541 gaaaatgccg ggatttattc ccggcatttc agattgttaa caacgtgcgc tttgttcatg
    15601 ccggatgcgg cgtaaacgct ttgtccgacc cacaaaagca cgtaaattca atatattgct
    15661 gaaat
//
LOCUS       DAZKIV010000107        15529 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZKIV010000107 DAZKIV010000000
VERSION     DAZKIV010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.107"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1168
                     /locus_tag="ACHOT6_003294"
                     /pseudo
     CDS             1..1168
                     /locus_tag="ACHOT6_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564134.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1261..1857)
                     /locus_tag="ACHOT6_003295"
     CDS             complement(1261..1857)
                     /locus_tag="ACHOT6_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948460.1"
                     /translation="MAVIPMSYSPATVARRFSILDGVTIQGVLYQIIWDPKTPFAAVI
                     EAAPSVIDGDIRHKVVATLELQRRSQLEGVFVRKFWEEQDVAQIEGIVVDGAVRDVGL
                     ATFVYETVATKAGVILLSDNEQYEGGKALWQHIARRSTNLKVFILDTDSARYYPFDGD
                     RICYDGESIPESEIWSEHPDRNKHGVVLVAESVNGKAA"
     gene            complement(1987..3303)
                     /locus_tag="ACHOT6_003296"
     CDS             complement(1987..3303)
                     /locus_tag="ACHOT6_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9948461.1"
                     /translation="MAVNLTETAIRALKAKKTSFYVWSNSSQRGTGRLGVKVQSSGSK
                     IFYFRYYVEKGTKERFIQLGIWPEMKLATANELAKKYGAWLIEGKDPQTELERQQLAE
                     QQRRQLHQSQGSFEALIHGYVNKMKIDNKRTWQDVLKRLENECYTVIPRETKAKDVTS
                     GQIKHILAGIIQRGAVVHSNRIRSYLMAAFNYGLKADNDPMNTSVGISFGLEANPVSV
                     IPKQSSAEKVGDTWLTLEELRFLMEHFAEATNVGLLMQHLIRLCIYTGGQRPFEMIAS
                     QWCDVDFQQKTLLVTADVSKNKREHLIPLTESALQELSCVQELTKEKSSPYIFPLSTN
                     GKRPVRTDSLARAIMYFRACNPDFKIFTARDLRRTCKTLMGEAGISKEIRDRIQNHAL
                     NDVSSKHYDRYDYLPEKRRALEIWEDRVNNYQQQTGNNVVNLFGRR"
     gene            complement(3600..4364)
                     /gene="nfeF"
                     /locus_tag="ACHOT6_003297"
                     /gene_synonym="yqjH"
     CDS             complement(3600..4364)
                     /gene="nfeF"
                     /locus_tag="ACHOT6_003297"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFP9948462.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRSEAEQYDPQRVRAAAYWHAK"
     gene            4641..5264
                     /gene="nfeR"
                     /locus_tag="ACHOT6_003298"
                     /gene_synonym="yqjI"
     CDS             4641..5264
                     /gene="nfeR"
                     /locus_tag="ACHOT6_003298"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFP9948463.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(5418..6938)
                     /gene="aer"
                     /locus_tag="ACHOT6_003299"
     CDS             complement(5418..6938)
                     /gene="aer"
                     /locus_tag="ACHOT6_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFP9948464.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            7356..8735
                     /gene="ygjG"
                     /locus_tag="ACHOT6_003300"
     CDS             7356..8735
                     /gene="ygjG"
                     /locus_tag="ACHOT6_003300"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFP9948465.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(8777..9109)
                     /locus_tag="ACHOT6_003301"
     CDS             complement(8777..9109)
                     /locus_tag="ACHOT6_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFP9948466.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            9328..10311
                     /gene="ebgR"
                     /locus_tag="ACHOT6_003302"
     CDS             9328..10311
                     /gene="ebgR"
                     /locus_tag="ACHOT6_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFP9948467.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            10495..13587
                     /gene="ebgA"
                     /locus_tag="ACHOT6_003303"
     CDS             10495..13587
                     /gene="ebgA"
                     /locus_tag="ACHOT6_003303"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFP9948468.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALNLTGGELKVENKLWFTT
                     LNDYTLHVEVRAEGETLATQQIKLLDVAPNSEAPLQITLPQLDAREAFLNIMVTKDSR
                     TRYSEAGHPIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            13584..14033
                     /locus_tag="ACHOT6_003304"
     CDS             13584..14033
                     /locus_tag="ACHOT6_003304"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFP9948469.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            14096..15528
                     /locus_tag="ACHOT6_003305"
                     /pseudo
     CDS             14096..15528
                     /locus_tag="ACHOT6_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         3958 a         3998 c         3749 g         3824 t
ORIGIN      
        1 tgatttattt aatattctga ctgaatgtaa agcctggagt tcaccaattc cagcagaagc
       61 ccggcagggg tattgccatc aggatctgtc tgttgcgcac tcctgcacct cagagttgct
      121 ggcacacctg aagcagtatc atgaaataga tgaatcaaca atacttgcca taaccattgg
      181 tctggcggtc aacgaattct accagtataa gcaaaatatt ccagaatggc tttcctgttt
      241 tgagaataag aacaaatggc cctgggactg caggaagtca gatactgaag atgtgcacag
      301 actgagagaa cgactatcga taatcggatg tacagaattc gtaaaaaata tgttctcagt
      361 cgccagtcag catgaaataa agacaatgta caatatttat gtccatattt atgcagctga
      421 aatctgcggt tgggtaaaaa caagcatggg catattttaa accaacagat ttcatccagt
      481 acacatggaa attcgcaaca gttactccgg ataaagatga aaaaaccgag aatacgaccg
      541 catgggaaag ggattattgt cagataacca tacaacgaga tgaactcctt agattgattc
      601 gcactaatac tctgcaggat gcagcaattc atctttcagg ctgcctgcaa tatgcatatc
      661 tggagtctgt aaagaatgat attgactatc ctggaatagc ccctaatgct gaagaaaaga
      721 aaatgaaata cacagattct attgaatttt ctggaattag gaataacatc ataaatgcag
      781 taaaacggct ggaatcattt gaaaaatggt atgaaactaa taaagttttg ttggtaagta
      841 ataaaacaat acgaacagag aagattgacg ttgttgtacg acttgcggga ctgaaagcct
      901 atgatttgca ggaaggggtt cctgatggtc tgaaatggaa agttaaagat ggcataaatg
      961 aacgcatcaa aaccgatacc agtttatgcc tgcctcagga tatcagtgat acatcgctaa
     1021 accgttatcg tcacgctgtg acgcgcattg tcaacgacga aatcgacaca ttactgatgg
     1081 aacagaagaa aaaaataaaa gattccccta cagcaaagat cgacatgcga tccaaccttt
     1141 atggagcaaa tgcgtgatcg agcagtagag ttctgagggg ataaccgagc tgttatcccc
     1201 cgtttattct gccaactctc aggtctgtca gagttccaag aatacgatgc ggctggcaaa
     1261 ttatgcggct tttccattaa cagattcggc cacaagaaca acaccgtgtt tatttcgatc
     1321 aggatgttca ctccagattt ctgattcagg aatactttcc ccgtcatagc agatccgatc
     1381 gccatcaaac ggataatacc gtgcggaatc agtatctaaa ataaacactt tcagattcgt
     1441 tgaacgacgc gctatatgct gccagagggc tttacctcct tcatattgct cgttatcact
     1501 taatagtata acgcccgctt ttgtggctac cgtttcatat acaaacgtcg caaggccaac
     1561 atcacgtaca gccccatcaa caacaatccc ttcaatctga gcgacgtcct gctcttccca
     1621 gaatttccgg acaaacaccc cttcaagctg tgagcgacgc tgaagctcca gagtggcaac
     1681 aactttatgg cgaatatcac catcaataac agaaggtgca gcttcgatta ccgcagcgaa
     1741 cggagtcttt ggatcccaga tgatctggta aagcacgccc tgaatagtta ctccatccag
     1801 tatcgaaaaa cgacgagcta cagtagcagg ggagtaagac attggaatta cagccatttt
     1861 tgaatcccct ctgagtaata caggcacgaa aatttacctc aataattata gtaaacaata
     1921 caagaactag gtgcatcaac aatacccatc aattgtacgc aattcgtaca gtaaccagga
     1981 actccctcac cttctgccaa acagattgac aacattattg cctgtttgct gttgatagtt
     2041 attaacccga tcctcccaga tctcaagtgc tctgcgtttt tctggcaaat aatcgtatcg
     2101 atcataatgt tttgaactga catcgttcaa cgcatgattt tgaatacgat cgcggatttc
     2161 cttactgatt ccagcttctc ccattaacgt tttacaggtc cgacgtaagt cacgtgctgt
     2221 aaaaatttta aagtcaggat tacaagcccg gaaatacatg atagcgcgag ccagactgtc
     2281 cgtacgaacc ggacgcttac cattagtcga aagcgggaaa atgtaagggc tcgatttttc
     2341 ttttgttaac tcctgcacac aggataattc ctgtaatgct gattcagtca acggtatcaa
     2401 atgctctcgt ttatttttag agacatccgc agtcaccagc agcgtttttt gctgaaaatc
     2461 aacatcacac cactgactgg cgatcatttc aaagggacgt tgccctccgg tatagataca
     2521 taatcgaatc aaatgttgca tcaataatcc aacgttggtt gcttcagcaa aatgttccat
     2581 tagaaaacgc aactcttcga gtgtcagcca ggtgtcaccg actttttctg cagatgactg
     2641 tttcggtatc actgatacag gattggcctc cagcccaaaa ctaatcccta cacttgtgtt
     2701 catcggatcg ttatcagctt tcagaccata gttaaatgca gccatcaggt aagaacggat
     2761 ccggttagag tgaaccacag caccgcgctg aatgatcccg gccaggatat gtttaatctg
     2821 tccggaagtc acatctttag ctttagtctc acgggggatc actgtatagc attcattttc
     2881 aaggcgtttc agaacatcct gccatgttct cttattatca attttcattt tgttaacata
     2941 accatgaatc aatgcttcaa atgaaccctg tgattgatgg agttgtctcc gctgctgttc
     3001 tgcaagctgt tgtcgctcca gttctgtctg gggatccttt ccttcaatga gccaggcacc
     3061 atactttttt gccagttcgt tcgctgtggc cagtttcatc tcaggccata tacccaattg
     3121 aatgaatctt tcttttgtcc ctttttccac gtaatagcgg aaatagaaaa ttttgctgcc
     3181 agaagactga actttgacgc caagtcgtcc agtgccacgt tgagagctgt tgctccagac
     3241 atagaacgat gtcttttttg cttttaatgc tcgaatagct gtttcagtta gattgacggc
     3301 catagaacct cttttctaaa tcagtaacaa tcaaaataac tcgtaaattt gggtcaggta
     3361 atgggtcagg taacgatcgt acaatggaga aaaaacggaa ccaagcggaa acagaagaaa
     3421 ttacataatt agttgattat aaacaattaa taaaacaatt acgaacaaac caaaacaata
     3481 cagaatggac ttgcctcatg actcataatc gcttggtcgc tggttcaagt ccagcagggg
     3541 ccaccagata tagcaaaggc tgacgagaaa tcgtcagcct ttttcttttt atatatcagt
     3601 tactttgcgt gccagtaagc cgctgcacgt acccgctgtg ggtcatactg ttccgcttca
     3661 gagcggcggc ttaaattctt aacgacttta ccttcgccgg ttatccagat gaagtaatca
     3721 tcggcaggga tttgcatctg cgccagacgc gcatctaccg cctgctcatc atgtgccagc
     3781 cattcgatat taaaaccatc aaggtgcgcg agataatcct gacaggcgtt atcccgcacg
     3841 ctaaccagcg cactaacttg cggtttaacg gcaagtttgc tcaacgtttc caggcggcgg
     3901 cgcagtgcgg gcattccgga ttcatcgcag acatacagct gatacgcgta atcttccggc
     3961 accaccagcg aaccgcgcgg acctgccacc gtaagtttat cgcccggttg cgcctgcatc
     4021 gcccagccgc tggcgacccc accgtcgtga ataaagaaat cgatcgccag ttcatggcgt
     4081 agttcgtcat acagcggcgt atagtcacgc gacggtgggc gtggtccttc cggccagacg
     4141 atgccctctt ccgttaccgt tggcggcaca aagtgagcgt caggttgagg aaagaagagt
     4201 ttgctgtgat cgtcaaagcc gcgcgatgta aaaccgtcca gcgcctcgcc gccgaggaca
     4261 atgcgctgaa aaccggcgct gatgcgctca acgcgtaaca cagtcagttc acggaagcgc
     4321 agatcattgc gaacgcgctg cgggtagcgg ggggagttat tcattgttat cgccttcgtg
     4381 atagtaatca gatatatcta aataattttc gtaaatgata atgattgtta atcatgataa
     4441 atgcaagcga tttgtagaac tgatatatct gaagtatgtg aaggcattca gtgccatagc
     4501 aatgctgccg gatgcggcgt gaacgtctta tccggcctgg atgtgaggtg cttccacgtt
     4561 taaaaatcat ttttgtactt gcagtaatgc tcacatcaga tataaattag atatatctaa
     4621 ttaagcaaaa ggaggctgat atgagccatc atcacgaagg gtgttgtaaa catgaaggcc
     4681 agccacgcca tgagggctgc tgcaaaggtg agaagtcaga acacgagcac tgcggacacg
     4741 gtcaccagca tgaacacggt caatgctgcg gtggtcgcca cggtcgcggc ggcggtcgtc
     4801 ggcaacgttt ctttggtcac ggtgaattgc gtctggtgat tctggatatt ctctcgcgcg
     4861 atgacagcca cggttacgaa ttgattaaag cgattgagaa cctaacccag gggaattaca
     4921 ccccaagccc gggcgtcatc tacccaacgc tggattttct gcaggagcag tcgctgatta
     4981 ccatccgcga agaggaaggt ggtaagaagc agattgcgct gaccgaacaa ggcgcgcagt
     5041 ggctggaaga aaaccgcgaa caggtggaga tgattgaaga acgcatcaaa gcgcgttgcg
     5101 ttggcgcggc gctgcgccag aacccgcaaa tgaagcgggc gctggataat tttaaagcgg
     5161 tgctggattt acgcgtcaac cagagcgata tcagtgatgc acaaataaaa aagatcattg
     5221 cggtgatcga ccgcgccgct tttgatatta cgcaactgga ttaatcgccg catccgccag
     5281 tggcgcggtg caattgccgg atgcgacgct tgacgcgcct tatccggcct acacccgcta
     5341 cacaccccgc aggcctgata agatgcgcca gcatcgcatc aggcattgtg ctccaaccgc
     5401 cggatccggc ataccgatta atgcagcacc gtcaccgcgt cttccagtcg gctggcgcgg
     5461 tgtttcacca tcgccgacac ctgcgcactc tcttccacca gctcggcatt tttctgggtg
     5521 atcaggttaa gctcatccac tgcacgggtc aggctggaaa gcccatcggc ctgttccagc
     5581 gttgaatggc taatctgggc gatcaactgg gtgacgtttt tcacctgtgc cacaatatct
     5641 tccatcgtcc gtccggcggc gtgtacctgc tgcgaaccgg attgcacctt atcagcactg
     5701 gcatcaatca gcttgcgaat atcgttggca gcattagcgc tgcggctggc taaatgacgc
     5761 acttcccctg ccaccactgc aaaacctttg ccctgttcgc cggcacgcgc cgcttccacc
     5821 gccgcattca gggccagaat attggtctga aacgcaatat cgttaatcag cgaagtaatg
     5881 gtgccaatgc gctgggtact gtcggcgata tcgtccattg tcttgatcac cgtggtcatc
     5941 gcctccccac cctgcactgc cgcattactg gcggtgatag aaagtttatc ggcagccgac
     6001 gccgtggcag agttctgttt caccgacgcc gccatttggt tcatggtcgc caccgtttgc
     6061 tgaacgttat caactgtctg ctgggtatgt tcgttcagtt catcggtgcc tttcgccagc
     6121 gtctcactgc catttctgac actggacacc tggcttgaga catcgttaat tagccaacgg
     6181 cacatcaggc caagctgccc taccgcgcgt aatgtcagcc ccagctcatc gctgcgattc
     6241 aggtgctcaa cactattacg ctctccggtc gccaccttca gtgcctgacg ggcgacattt
     6301 tctatcgggc gcacaatctg ccattcaaaa caagcgcttg ccaacaatac cactaacaca
     6361 cagaggaaat acgtcaccac cggggcagca acaaaccaaa gcatggccgc cagcaagata
     6421 aacatcaggg tcatcactcc acgcgcccgc cagcgaagcg gtaatgaagg cagtttaccc
     6481 agccagcctt tacgcaccac caggccttta tgaatacgct tactggtacg tccggcgttc
     6541 agcgctttgt acagcggctc caccgccgca atctcttcat ccgtcgcccg ggtacgaatc
     6601 gacatatagc cactgatttt tccctcgcgc accatcggta ccgcattggc ccgcacccaa
     6661 taatggtcgc catttttgcg gcgatttttc acgatgccgc tccagggctc cccttttttc
     6721 agggtgaacc acatatccgc aaacgccgct tttggcatat ccgggtgacg caccatgttg
     6781 tgcggctgcc cttgtaactc ttgcaaggta aagccgctca cctgcacaaa agtgtcatta
     6841 gcatgagtga tatagctttg cagatcggta gtggacatca gagtggtatc gtccgccagc
     6901 ggggtatttt gctgggtgac atacggatga gaagacatgt tcgcgtcctg tgcaggttat
     6961 atggttgtta acttcttgtc agagtttatg tcggccccgc tgcggttatc tttaaccgat
     7021 taatttgatt tagatcgcaa tttgcgatta aagcacaaat ctaattcttt gatttaaaat
     7081 actttcactc tgttactata cgaaaacgtt aattatcttg cccaaaaatc aggcaattat
     7141 tgccctgaaa acgtgcattt gcgcagcaat catcaaatcc atacccgaca aaaaccgtgc
     7201 aaaataacaa caaatgttaa cagatagcat taaatattgc acaaatgata accgaatttg
     7261 tgtttatccc gattttcgcg atcgcagccg gagtggcgca atccctgcaa tacttaaatc
     7321 ggtatcatgt gatacgcgag cctccggagc atattttgaa caggttacct tcgagcgcat
     7381 cggctttagc gtgcagcgcc cacgccctga atctcattga aaagcgaacg ctggatcatg
     7441 aggagatgaa agcacttaac cgagaggtga ttgaatactt caaagagcat gtcaatccgg
     7501 ggtttttaga gtatcgcaaa tctgttaccg ccggcgggga ttacggagcc gtagagtggc
     7561 aagcgggaag tttaaatacg cttgtcgaca cccagggaca ggagtttatc gactgcctgg
     7621 gaggttttgg aattttcaac gtggggcacc gtaatccagt tgtggtttcc gccgtacaga
     7681 atcaacttgc gaaacaaccg ctgcacagcc aggagctgct cgatccgtta cgggcgatgt
     7741 tggcgaaaac ccttgctgcg ctaacgcccg gtaaactgaa atacagcttc ttctgtaata
     7801 gcggcaccga gtccgttgaa gcagcgctga agctggcgaa agcttaccag tcaccgcgcg
     7861 gcaagtttac ttttattgcc accagcggcg cgttccacgg taaatcactt ggcgcgctgt
     7921 cggccacggc gaaatcgacc ttccgcaaac cgtttatgcc actgctgccg ggcttccgtc
     7981 atgtgccgtt tggcaatatc gaagccatgc gcacggctct taacgagtgc aaaaaaaccg
     8041 gtgatgatgt ggctgcggtg atcctcgaac cgattcaggg tgaaggtggc gtaattctgc
     8101 cgccgccggg ctatctcacc gccgtacgta agctatgcga tgagttcggc gcactgatga
     8161 tcctcgatga agtacaaacg ggcatggggc gcacgggcaa gatgttcgcc tgcgagcatg
     8221 agaacgtaca gccggatatc ctctgccttg ccaaagcgct cggcggcggc gtgatgccga
     8281 ttggcgcgac catcgccact gaagaggtgt tttcagttct gttcgacaac ccattcctgc
     8341 ataccaccac ctttggcggc aacccgctgg cctgtgcggc ggcgctggcg accatcaatg
     8401 tgttgctgga gcagaactta ccggctcagg ctgagcaaaa aggcgatatg ttgctggacg
     8461 gtttccgtca actggcgcgg gaatatcccg atctggtaca ggaagcgcgt ggtaaaggga
     8521 tgttgatggc aattgagttt gttgataacg aaatcggcta taactttgcc agcgagatgt
     8581 tccgccagcg cgtactggtg gccggaacgc tcaataacgc caaaacgatc cgcattgaac
     8641 cgccactgac actgaccatt gaacagtgtg aactggtgat caaagcggcg cgtaaggcgc
     8701 tggcggccat gcgagtaagt gtcgaagaag cgtaatatca aatcggatgg cgatacgacg
     8761 tcgccatccg atctttttat acgacgcgta cgcccgcagg catcatccgc tccggcgtta
     8821 acaacacgct ttcactgccg tcatcggttt ccgcacacaa cagcatgcat tccgacgttt
     8881 caccacgcat tttcgctttt tgcagattgc ataagaccac caccgttttc cccatcagct
     8941 cttcttcgct gtagtacggc accaggctgg tcacggtttg cagtgttttt tcccccacat
     9001 caacctgtac gatgtacagc ttgtcggcgt tttcatggcg tttcacttcc acaatctttc
     9061 cgacgcgcat ttccagacgt gcaaaatcag cgtaagccac ggtttccatt gtttcctccc
     9121 ttcgggtaaa attttactaa actatagcaa aagtttttct caatcctgta ggctaaaaat
     9181 ggagaatgca ggcgtgatca cattcgtgag ccgctgtgtt accgttacag cgtcaaagaa
     9241 acgcgcttta tttactgaaa acaggtgacc cgataagcac ttcctctaca atgggggcgc
     9301 acatcaggga aagtaaaaaa ggtaaacatg gcaacactaa aagacatcgc aatcgaagct
     9361 ggcgtatccc tggcgacagt atccagggtc ttaaatgacg atccgacatt gaatgtgaaa
     9421 gaagagacga aacatcgcat tctcgagatc gccgaaaagc tggagtacaa gaccagtagt
     9481 gcccgtaaac tccagacagg tgcagtcaac caacaccata ttctggctat ctacagctac
     9541 cagcaggagc tggagatcaa cgatccttac tatctggcga tccgccacgg cattgaaacc
     9601 cagtgcgaaa agctgggcat cgagctcacc aactgttatg aacacagcgg cttaccagac
     9661 attaaaaacg tcaccggtat tttaattgtc ggcaaaccca cgcccgccct gcgcgccgct
     9721 gccagcgcgt tgaccgacaa tatctgtttt atcgactttc acgaacccgg cagcggttac
     9781 gatgcggtgg atatcgatct ggcacgcatc agtaaagaaa tcatcgactt ctatatcaat
     9841 cagggcgtta atcgtattgg ttttattggc ggtgaagatg agccaggcaa agcggatatt
     9901 cgtgaggtcg cctttgcgga atatggccga ctgaaacaag tggtacgcga agaggatatc
     9961 tggcgcggag gcttttccag ttcgtcgggt tatgaactgg caaaacagat gctggcgcag
    10021 gaagactatc cgaaggcact gtttgttgct tccgattcca ttgctatcgg cgtactgcgg
    10081 gcaattcatg aacgaggcct gaacatccca caggatattt cgcttatcag cgttaacgat
    10141 atccccaccg cgcgatttac ctttccgccg ctctccaccg tgcgcatcca ttccgagatg
    10201 atgggaagtc agggcgttaa cctggtgtat gaaaaagccc gcgatggtcg cgcgctgccg
    10261 ctgttagtct tcgttcccag caaattaaaa ctgcgcggca cgacccgtta aatcccctta
    10321 cacactgtcc ggcaatcgtt tttgccggac agtgctgccg tttattttcg tgatccagtt
    10381 aaagtaaatg catttacctg ctacttttta gtaaaaattt tactaaactc cccagcaatt
    10441 acacaaacta ccatcaccat gaatggttcc gatttctctc taccgggagg ccctatgaat
    10501 cgctgggaaa acattcagct cacccacgaa aaccgacttg cgccgcgtgc gtactttttt
    10561 tcatatgatt ctgttgcgca agcgcgtacc tttgcccgcg aaaccagcag cctgtttctg
    10621 cccttaagcg gtcagtggaa tttccacttt tttgaccatc cgctgcaagt accagaagcc
    10681 ttcacctctg agttaatggc tgactggggg catattaccg tccccgccat gtggcaaatg
    10741 gaaggtcacg gcaaactgca atataccgac gaaggttttc cgttccccat cgatgtgccg
    10801 tttgtcccca gcgataaccc aaccggtgcc tatcaacgta ttttcaccct cagcgacggc
    10861 tggcagggta aacagacgct gattaaattt gacggcgtcg aaacctattt tgaagtctat
    10921 gttaacggtc agtatgtggg tttcagcaag ggcagtcgcc tgaccgcaga gtttgacatc
    10981 agcgcgatgg ttaaaaccgg cgacaacctg ttgtgtgtgc gcgtgatgca gtgggcggac
    11041 tctacctacg tggaagacca ggatatgtgg tggtcagcgg ggatcttccg cgatgtttat
    11101 ctggtcggaa aacacctaac gcatattaac gatttcactg tgcgtaccga ctttgacgaa
    11161 gcctattgcg atgccacgct ttcctgcgaa gtggtgctgg aaaatctcgc cgcctcccct
    11221 gtcgtcacga cgctggaata taccctgttt gatggcgaac gcgtggtgca cagcagcgcc
    11281 attgatcatt tggcaattga aaaactgacc agcgccagct ttgcttttac tgtcgaacag
    11341 ccgcagcaat ggtcagcaga atccccttat ctttaccatc tggtcatgac gctgaaagac
    11401 gccaacggca acgttctgga agtggtgcca caacgcgttg gcttccgtga tatcaaagtg
    11461 cgcgacggtc tgttctggat caataaccgt tatgtgatgc tgcacggcgt caaccgtcac
    11521 gacaacgatc atcgcaaagg ccgcgccgtt ggaatggatc gcgtcgagaa agatctccag
    11581 ttgatgaagc agcacaatat caactccgtg cgtaccgctc actacccgaa cgatccgcgt
    11641 ttttacgaac tgtgtgatat ctacggcctg tttgtgatgg cggaaaccga cgtcgaatcg
    11701 cacggctttg ctaatgtcgg cgatatcagc cgtattaccg acgatccgca gtgggaaaaa
    11761 gtctacgtcg agcgcattgt tcgccatatt cacgcgcaga aaaaccatcc gtcgatcatc
    11821 atctggtcgc tgggcaatga atccggctat ggctgtaaca tccgcgcgat gtaccacgca
    11881 gcgaaggcgc tggatgacac gcgactggtg cattacgaag aagatcgcga tgctgaagtg
    11941 gtcgatatta tttccaccat gtacacccgc gtgccgctga tgaatgagtt tggtgaatac
    12001 ccgcatccga agccgcgcat catctgtgaa tatgctcatg cgatggggaa cggaccgggc
    12061 gggctgacgg agtaccagaa cgtcttctat aagcacgatt gtattcaggg acattatgtc
    12121 tgggagtggt gcgaccacgg gatccaggcg caggatgaca acggcaatgt ctggtataaa
    12181 ttcggcggcg actacggcga ctatcccaac aactataact tctgtcttga tggtttgatc
    12241 tattccgatc agacgccggg accgggcctg aaagagtaca aacaggttat cgcgccggta
    12301 aaaatccacg cgctaaatct gactggcggc gagttgaaag tcgaaaataa actatggttt
    12361 accacgctta atgactacac cctacacgta gaggtgcgcg ccgaaggtga aacgctcgcg
    12421 acgcagcaga ttaaactgct cgacgttgcg ccgaacagcg aagccccctt gcagatcacg
    12481 ctgccgcaac tggacgcccg cgaagcgttc ctcaacatta tggtgaccaa agattcccgc
    12541 acccgctaca gcgaagccgg acaccctata gccacttatc agttcccgct gaaggaaaac
    12601 accgcgcaac cagtgccttt cgcaccaaat aatgcgcgtc cgctgacgct ggaagacgat
    12661 cgtttgagct gcaccgttcg cggctacaac ttcgcgatca ccttctcaaa aatgagtggc
    12721 aaaccgacat cctggcaggt aaatggcgag tcgctgctga cccgcgagcc aaagatcaac
    12781 ttcttcaagc cgatgatcga caaccacaag caggagtatg aagggttgtg gcagccgaat
    12841 catttgcaga tcatgcagga acatctgcgc gattttgccg tagaacagag cgatggtgaa
    12901 gtgttgatca tcagccgcac agttattgca ccgccggtgt ttgacttcgg gatgcgctgt
    12961 acctacatct ggcgcatcgc tgccgatggc caggttaacg tggcgctttc cggcgagcgt
    13021 tacggcgact atccgcacat cattccgtgc atcggtttca ccatggggat taacggcgaa
    13081 tacgatcagg tggcgtatta cggtcgtgga ccgggcgaaa actacgccga cagccagcag
    13141 gctaacatca tcgatatctg gcgcagcacc gtcgatgcca tgttcgagaa ctatcccttc
    13201 ccgcagaaca acggtaaccg tcagcatgtc cgctggacgg cactgactaa ccgccacggt
    13261 aacggtctgc tggtggttcc gcagcgccca attaacttca gcgcctggca ctatacccag
    13321 gaaaacatcc acgctgccca gcactgtaac gagctgcagc gcagtgatga catcaccctg
    13381 aacctcgatc accagctgct tggcctcggc tccaactcct ggggcagcga ggtgctggac
    13441 tcctggcgcg tctggttccg tgacttcagc tacggcttta cgttgctgcc ggtttctggc
    13501 ggagaagcta ccgcgcaaag cctggcgtcg tatgagttcg gcgcagggtt cttttccacg
    13561 aatttgcaca gcgagaataa gcaatgagga tcatcgataa cttagaacag ttccgccaga
    13621 tttacgcctc tggcaagaag tggcaacgct gcgttgaagc gattgaaaat atcgacaaca
    13681 ttcagcctgg cgtcgcccac tccatcggtg actcattgac ttaccgcgtg gagacagact
    13741 ccgcgaccga tgcgctattt accgggcatc gacgctattt tgaagtgcat tactacctgc
    13801 aagggcagca aaaaattgaa tatgcaccga aagagacatt acaggtagtg gaatattatc
    13861 gtgatgaaac tgaccgtgaa tatttaaaag gctgcggaga aaccgttgag gtccacgaag
    13921 ggcaaatcgt tatttgcgat atccatgaag cgtatcggtt tatctgcaat aacgcggtca
    13981 aaaaagtggt tctcaaagtc accatcgaag atggttattt ccataacaaa taacaactac
    14041 ggcggcaaaa ggagtttgcc gccaccgcta ccctacacat tttcggagat gtgttatgtc
    14101 tgataccaaa cgtaatacaa tcggcaaatt cggcttgctc tcgctgactt ttgccgccgt
    14161 ttacagcttt aacaacgtta tcaataataa tattgagctt ggactggcct cggcaccgat
    14221 gtttttcctc gcgacgattt tttattttat tcccttctgt ctgatcatcg cagaatttgt
    14281 ttcgttaaat aaaaactcag aagccggtgt ctacgcgtgg gtaaaaagtt cgctgggcgg
    14341 acgttgggca tttattactg cctataccta ctggttcgta aacctgttct ttttcacctc
    14401 actgttgccg cgcgttattg cttatgcttc gtatgccttc ctcggatacg aatatattat
    14461 gacgccggtt gccaccacca ttatcagtat ggtgctgttc gccttctcca cctgggtttc
    14521 caccaacggg gcgaaaatgt tggggccaat tacctccgtc acttcaacgc tgatgctgct
    14581 gttaacgctc tcctacattt tactggcagg tacggcgctg gttggcggcg tacagcctgc
    14641 tgaccccatc accgttgacg cgatgatccc gaacttcaac tgggcgttcc tcggcgttac
    14701 cacctggatc tttatggccg caggtggcgc ggagtccgtc gctgtgtacg ttaacgacgt
    14761 caaaggcggt tcgaaatcgt tcgttaaagt gatcatcctc gccgggattt ttatcggcgt
    14821 actgtattcc gtctcctcgg tgctgattaa cgtcttcgtc agcagcaaag agttgaaatt
    14881 taccggcgga tcggtgcagg tattccacgg catggcggcg tattttggtc taccggaagc
    14941 gttgatgaat cgctttgtcg gtctggtgtc ctttaccgcg atgttcggtt ccctgctgat
    15001 gtggaccgca acgccggtga aaattttctt ctccgaaatc ccggaaggca tctttggtaa
    15061 gaaaaccgtc gaactgaacg aaaacggcgt tccggcgcgc gcagcgtgga tccagttcct
    15121 gatagtcatc ccgctgatga ttatcccgat gctcggttcc aataccgtgc aggatctgat
    15181 gaatactatt attaatatga ccgccgcagc gtccatgctt ccgccgttat tcatcatgct
    15241 ggcttacctg aatttacgcg ccaaattaga tcacctccac gcgatttccg tatgggctcc
    15301 cgccgcaccg gtattatcgt tgtttcaatg ctgattgcga tatttgccgt agggtttgtc
    15361 gcttcgacat tcccgactgg cgcgaatatt ctgaccatca ttttttataa cgtcggcggt
    15421 attgttatct tccttggctt tgcgtggtgg aaatacagta aatatataaa gggattaacg
    15481 gctgaagagc gccatattga agcgacgcca gccagcaatg ttgattaag
//
LOCUS       DAZKIV010000108        15522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZKIV010000108 DAZKIV010000000
VERSION     DAZKIV010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.108"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..191)
                     /locus_tag="ACHOT6_003306"
     CDS             complement(<1..191)
                     /locus_tag="ACHOT6_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HFP9948470.1"
                     /translation="MLSLYEKIKIRLIILFLLAALSFIGLFFIISYQLVSERAVKRAD
                     SRFELIQKNVGYFFKDIERS"
     gene            complement(369..1280)
                     /gene="gsiD"
                     /locus_tag="ACHOT6_003307"
     CDS             complement(369..1280)
                     /gene="gsiD"
                     /locus_tag="ACHOT6_003307"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFP9948471.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1283..2203)
                     /gene="gsiC"
                     /locus_tag="ACHOT6_003308"
     CDS             complement(1283..2203)
                     /gene="gsiC"
                     /locus_tag="ACHOT6_003308"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFP9948472.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2221..3759)
                     /gene="gsiB"
                     /locus_tag="ACHOT6_003309"
     CDS             complement(2221..3759)
                     /gene="gsiB"
                     /locus_tag="ACHOT6_003309"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFP9948473.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKVQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3779..5650)
                     /gene="gsiA"
                     /locus_tag="ACHOT6_003310"
     CDS             complement(3779..5650)
                     /gene="gsiA"
                     /locus_tag="ACHOT6_003310"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFP9948474.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLELAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     ALKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPCNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5637..6602)
                     /gene="iaaA"
                     /locus_tag="ACHOT6_003311"
     CDS             complement(5637..6602)
                     /gene="iaaA"
                     /locus_tag="ACHOT6_003311"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFP9948475.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6806..8041
                     /gene="moeA"
                     /locus_tag="ACHOT6_003312"
     CDS             6806..8041
                     /gene="moeA"
                     /locus_tag="ACHOT6_003312"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFP9948476.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VEVGEWVEVEPFNALFGGL"
     gene            8041..8790
                     /gene="moeB"
                     /locus_tag="ACHOT6_003313"
     CDS             8041..8790
                     /gene="moeB"
                     /locus_tag="ACHOT6_003313"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFP9948477.1"
                     /translation="MTELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDSVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8866..9528)
                     /gene="fsa"
                     /locus_tag="ACHOT6_003314"
     CDS             complement(8866..9528)
                     /gene="fsa"
                     /locus_tag="ACHOT6_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFP9948478.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9659..10558
                     /gene="ybiY"
                     /locus_tag="ACHOT6_003315"
     CDS             9659..10558
                     /gene="ybiY"
                     /locus_tag="ACHOT6_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFP9948479.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10564..12996
                     /gene="ybiW"
                     /locus_tag="ACHOT6_003316"
     CDS             10564..12996
                     /gene="ybiW"
                     /locus_tag="ACHOT6_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFP9948480.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13142..13957
                     /gene="ybiV"
                     /locus_tag="ACHOT6_003317"
     CDS             13142..13957
                     /gene="ybiV"
                     /locus_tag="ACHOT6_003317"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFP9948481.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14109..15374
                     /gene="ybiU"
                     /locus_tag="ACHOT6_003318"
     CDS             14109..15374
                     /gene="ybiU"
                     /locus_tag="ACHOT6_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFP9948482.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLEDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3781 a         4283 c         3940 g         3518 t
ORIGIN      
        1 gaacgttcaa tatctttaaa gaaatagcca acgtttttct gaataagttc aaagcggcta
       61 tcggcacgtt ttaccgcgcg ttccgatacc agttgatagc taatgatgaa aaaaagacca
      121 ataaatgaca gtgctgccaa taaaaataaa attatcagcc ttatctttat cttttcgtat
      181 aaactcagca tgttaataca tttaacaaaa taactatctg aacgctgtgc cgctgcaaaa
      241 atagtccctt gctgcggctt tttaaccaaa ttacccgatt ctacaaaatc agcagaaagt
      301 tacaacactt gcttagggca ataatttcct gtgaggtgat taccctttca agcaatattc
      361 aaacgtaact atcctttaat tttcggatcc agcgcatcgc gtaaaccatc gcccaacaaa
      421 ttgaacgcca gtacggtcag aaaaatagcc agggccggaa aaacagcgac atgcggcgcg
      481 ataaccatat ccgctcgtgc ctcattgagc attgctcccc actctggtgt cggcggctgc
      541 gcaccgaggc cgagaaatga caggctggcg gcagagataa tcgaggtgcc aatgcgcatg
      601 gtgaaaaaca ccacgataga cgagacggtc ccaggcaaga tatgacgcaa taaaatggtc
      661 atatcgctgg caccaatact gcgtgctgac tcaataaagg tttgctgttt caacaccagc
      721 gtgttgccgc gcaccaggcg ggcaaacgcg gggatggaaa aaatggcgac tgcgataatc
      781 acgttagcaa tgccgcttcc caacaccgca acaacagcga tcgccagtaa aatacccggg
      841 aaggcaaaca gcacatcgca aatgcgcatg atcagccgat cccaccagcc ttcataatat
      901 ccagccaaca agcccagcaa cgtccctatc gccgcaccga taaacacggc aaacacgccc
      961 gccgccagcg agatttgcgc accaaccagg acacggctga aaatgtcgcg ccccagtgaa
     1021 tcgacgccaa accagtgctg caaagaaggt ccattattca gattgtcata atcaaaataa
     1081 ttttcggcgt catagggagc gatccagcgt gcaaaaatgg ccaccacaat caataaaata
     1141 acgaataatg cggcggtcat cgccatatgc tgacggcgaa atcgtcgcca gaattcatgc
     1201 cacggtgtac gcacctggtc aggtttgacc agtggcatgg cgtttaacac cgcctgacgt
     1261 cgccagttaa atagtcgcat ccttacttgt acctgatagc cgggttaatg gcggcgtaaa
     1321 gcacatccac cactaagttg ataagaataa attccagcga gaaaagcaga atttccgcct
     1381 gaatcaccgg gtaatcgcgc atttctacgg agtcaacgag taagcgtcca agtccgggcc
     1441 agttgaaaac tttctccacc acgatagaac caccgagcaa aaagccaaac tgtaagccca
     1501 tcatggtcac taccgggatc atcgcgttac gtagcccgtg tttgaggaca acccaggttt
     1561 cgctcacccc tttcgccctc gcggtacgca tataatcttc gcttaaaaca tcgacaaacg
     1621 acgcgcgggt aaagcgcgcc atcacggctg ccactgccgc gccgagcgtc agggagggta
     1681 aaatgtagtg ctgccaactg tctgctccca cggtaggcag ccagcccagt tcaacggaga
     1741 atacctgaat taaaagcatc cccagcgcaa atgccggaaa cgagatcccc gacaccgcaa
     1801 tggtcatact caatcgatcc ggccaacgat tacgccagac ggcggcgata attcccgccg
     1861 ccataccaaa tatcaccgcc cagaccatac tggttatggt cagccacagc gttggcataa
     1921 agcggctggc aatctcatcg gcaaccggac gacgcgacac catcgacagg ccaaaatccc
     1981 cctgcacagc attgctgata tagtgccaga actggtgata cagcggctga tccaacccca
     2041 gctgctgacg caccagttct ataacctgcg catcagcttc gggcccggca atcaatcgcg
     2101 ccggatcgcc gggcagcata tgcacaaata aaaacaccag caccgagacg ataaacagcg
     2161 tcggaatcaa ccccagtaag cgtttgataa cgtaattaag cattccactc cctgcgttgc
     2221 ttattgcaaa tccgcgtctt caaagctgaa gccggtgtct ggcatgatcc aaaaaccggt
     2281 caggttttta ctgtgtgccg acaccagttt ttctaccacc agcgggatcc acggcgactc
     2341 ttgccagatg atatcctgcg ccgccttata caagcgggtc ttttccgccg gatcattagt
     2401 tttcagtgcc tgagccagga agtcgtccac ctgtttattg ctgtaaaatg cggtattaaa
     2461 cagcgtcggt ggccagtttt gcgaggcgaa cagtggcgat agcgcccagt ccgcttcgcc
     2521 ggttgaagcc gaccagccag tgtagaacat ccgcacgccg ctctcttttt gccctttacc
     2581 ttcaacttct gccgcccgct gtccggcatc catcgcagtc acctggactt taatcccgac
     2641 ctgcgctaac tgctgctggg taaattgcag cactttctgc gcggtgctgt ggttatgtga
     2701 cgaccacagc gtggtactga aaccgttggg atatcccgcc tctttcagta attcgcgcgc
     2761 tttcactgga tcgtaaggcc acggtttata actttgcgcg taggcgatac ttggcggtac
     2821 cacaccagta gctggcgttg catagcctgc aaaggcaact ttcaccagcg ccggacggtt
     2881 aatggcgtaa ttcagcgcct cacggacctt cgggttatcg aacggctttt gcgtcacgtt
     2941 catactgata taacgctgca taattgacgg actggccatc aactcgatat ttttgttttt
     3001 ctccagcagt gtggcctgct cgtagggaat ggggaaagca aactgcgctt caccggtttg
     3061 cagcattgcc gcgcgggtgt tgttatccgc caccggacgc caggttatgc tgtccagttt
     3121 gggcaatcct ggctgccagt aacccgcgaa ttttttcacc ttcacaaaat cggtctgatt
     3181 ccaggtatcc agttcatacg gtccggttcc caccggatga aaaccaatct ccttgccata
     3241 tttttccagc gctgccggtg aaatcatcgc ggtcgccgga tgggcaagaa tattaataaa
     3301 cgctgagaac ggctgtttga gggtaatctt taccgttgtc ggatcgatcg cctccgtttt
     3361 ggcaatattc ttatacaggt tatagcgttt aagatgattc gccggatcgc tggcccggtc
     3421 cagattcgct ttcaccgccg cggcgttgaa atcggtgcca tcctggaatt taattccttc
     3481 ccgcaatttc acggtgtaag taatgccgtc atcggaaacg gtataactct ccgccagcac
     3541 gtttttcagt ttcatctctt tatccagacc gaacagcccc tggtaaaacg atttcgctac
     3601 ggcctgagat aacgtgtcat ttgcgtcata cggatcgagc gtggtgaaat tcgatcctac
     3661 cgccaccacc acatctttgg cagcgaatgc acaagatgcc atcaacgctg tcgcaatgcc
     3721 cagcgccact aacccactac ggtgtacagc tcttgccatt ttattctccg cctgaatgtt
     3781 atctacgcat gaatgcgtat tctgattgtg gttgtgcgac gtaatgcccc ggcccgacgc
     3841 attgcaacga gacggctgcc acctcttcgc cacgcagatg aatattgcag ggaagatcgt
     3901 ccgacagcag tacacgctgc ggtcgttgtc gggacggttc agcgaccgga actgccgcca
     3961 gtaatttacg cgtataagga tgctgcgggt tttcgaagac cgcgcgccgt ggaccaattt
     4021 caacaatttg cccgagatac atcaccgcca cacgatgact aatccgctct accacggcca
     4081 tatcgtggga gataaacaga tacgcaatgc cgaaatcacg ctggagatcg agcaacaagt
     4141 tgataatctg cccgcgaata gaaacatcca gcgccgaaac ggcttcgtcg gcaatgatca
     4201 cttttggatt caatgccaac gcgcgagcaa tgcagatgcg ctggcgctga ccgccggaaa
     4261 actcatgcgg gtaacgccag gcatgttcag gtaacaggcc cacgcgctcc agcaaccacg
     4321 caacgcgtgc agccgcgtct ttacctggca ataaaccgtg tacacgcagc ggttcgataa
     4381 tcgaatcacc gatggtctga cgtgggtcca gcgaagcgta agggtcctga aaaataaact
     4441 gaatatcccg gcgcaatgcc tgaagtttgc cgggtgacaa ggtatcgatt ctctgaccgt
     4501 taaagataat ttcgccgccc tgcgattcga ccaggcgcag caacgcccgc ccggtagtgg
     4561 atttaccgct gccagactcg cccaccagcg atagcgtttc gccaggccag agatcaaaac
     4621 tgactttctc aacggcatgc acttcccgcg ttacgcgatt caacaaaccg ctgcgcaaag
     4681 ggaaacgggt gacaagatta cgcactcgta aaacaggttc gccatccacc accgttttct
     4741 gctcgatggg gggggcctgt ttcgctggat gttcaagcga tatcaacggg aaacgtcggg
     4801 gataatctaa ccctttcaac gcaccaagtt gcggaacagc agctaacagc gcacgggtgt
     4861 aaggatgttg cggtgcatga aaaatctgtt cgacggtacc cgtttccacc gcctcgccct
     4921 gatacatcac cagtacccga tcggcaatct ctgccaccac gcccatatcg tgagtgataa
     4981 agataacgcc catcgacatc tctttttgca atactttgat taattgcagg atctgcgcct
     5041 gaatagtgac atccagcgcg gtggttggct catcggcaat cagcaccgcc gggcggcatg
     5101 acagcgccat cgcaatcatc actcgctggc gcatcccgcc agagagttga tgcggataac
     5161 gtgaaagaat ggtttgtgcc tcaggaatgc gtacctgatc cagcatccgc ttcgcctcga
     5221 ccatcgcttc ttcacgactg gcgttctgat gcagacgaat tgattcggca atctgttcac
     5281 ccacagtaaa taccgggttc agcgatgtca tcggctcctg aaatatcatc gccatatccg
     5341 caccgcgaac atggcgcatt tgtgcagcgc tctgctcgct aagttcaatc acttcgcgac
     5401 tgcgccgctg caacagcatt ttatcgcact gtactaaacc gcccgccagt tccaacaggc
     5461 gcatcaatgc caacgcagtc actgacttac cggagccgga ttcgccaaca attgccagcg
     5521 tctcaccgcg ttgcagacta aaagagagat tgcggaccgc agctattttc tgctggtcct
     5581 gcataaaggc aatattcaga ttttcaaccg ccagcacatt accggcatca agttcatcac
     5641 tgtgtggcaa cggtgtcccc tttttcacgg tagataccgg tggttggcgt atcgcctgcg
     5701 tagccccagg cgcgatacat tccttcggtg ttaaacggta gcgcgacatt cccttcatgg
     5761 tcgatagcga ttaagccacc gctaccgcca agcgcaggga gtttttccat tactacccgc
     5821 tcgcaggctt ccgcgagact taatccgccg taatccatta acgcggcgat gtcatatgcc
     5881 gccagcgcgc ggatgaagac ttcgcccgtg ccggtacaag aaaccgccac actggcgtta
     5941 ttggcgtagc atccggcacc cactaaggga ctatcgccaa ctcgtccggg taatttattg
     6001 gtcattccgc ctgtggacgt ggctgccgcc aaattgccgt ctaaatccaa cgccacggcc
     6061 cccacggtgc ccattttctg tttttcatcc agtggcgcac cgctatggtc gaggacggtt
     6121 gccccttcct tgcgcgctgc cagtagttgt tcataacgca aaggcgtgga gaaaatctcc
     6181 ggcgagacgc gctccatgcc acgagcaaac gcaaaatttt ctgccccttc gccaatcatc
     6241 atcacatgcg ggctttgctc catcaccagc cgggcggcaa gaaccggatt acgcagatga
     6301 ctaacgcccg ccaccgcacc ggctttcagg gtgttaccat ccatcacaca ggcgtccagt
     6361 tcatgggttt catcacgcgt aaagacagcg ccaattccgg cgttaaacag tggacactct
     6421 tccagcagac gcaccgcttc cgtcaccaca tccagcgcac tttcgcccgc ttccagcatt
     6481 ttctgcccgg tttcaacaat ggcagacaac gcctcaatgt agcgtaattc ctgttgcaga
     6541 ctcatctgcg cgcggctaat tgcacctgcg ccaccatgaa ttgcaatgac tgctttgccc
     6601 atagtattca tccattaagg gtggctcgcc acttttgcta taaatatcag aatttttatg
     6661 aattactcat tcaatttttg aatatagaaa gatggatatg gcatgtaaag gcaggagcca
     6721 acgctacgcc cgaggtggca tacagtctgt ggcgtttttc tgacataata ggcaaattcg
     6781 attttgcctc cgcaggagtg ttttcatgga atttaccacc ggattgatgt cgctcgacac
     6841 cgcgcttaat gagatgcttt ctcgcgtcac cccactgacc gcccaggaaa cgctgccact
     6901 ggtacagtgt tttggtcgta ttctggcgag cgatgtcgtt tcgccgcttg atgttccggg
     6961 gtttgataac tccgcaatgg acggctacgc ggtgcgttta gccgatattg cctccgggca
     7021 accgctgccc gttgccggta aatcctttgc cggtcagccg tatcatggtg aatggcctgc
     7081 gggcacctgc attcgtatta tgaccggtgc gccggtgccg gaaggctgcg aagcggtggt
     7141 gatgcaggag cagactgaac aaacggacaa tggcgtgcgt tttactgctg aagtgcgtag
     7201 cgggcaaaat attcgccgtc gcggtgaaga tatctctgca ggtgcggttg ttttcccggc
     7261 gggaactcgc ctgactaccg cagagctgcc agtaattgct tcactgggga ttgccgaagt
     7321 tccggtgatt cgtaaagtgc gtgtagcgct tttttctacc ggtgatgaac tccagttgcc
     7381 cggtcagccg ctgggcgacg gccaaatcta cgacaccaac cgtctcgccg tacacctgat
     7441 gttagaacag ctgggttgcg aggtaatcaa cttagggatt atccgcgacg atccccatgc
     7501 cctgcgcgcc gcatttattg aagccgacag ccaggcggat gtagtgatca gttccggcgg
     7561 tgtttcagtg ggtgaagcgg attacaccaa aacgattctt gaagagctgg gggagatcgc
     7621 cttctggaag ctggcgatta aaccaggtaa accgttcgcg ttcggtaaac tcagcaatag
     7681 ctggttctgc ggcctgccgg gcaacccggt ttcagcgacg ctgaccttct atcaactggt
     7741 acagcctttg ctggcaaaac taagcggcaa caccgccagc ggcctgcccg cgcgccagcg
     7801 cgtacgcaca gcgtcccgcc tgaaaaaaac gccaggacgt cttgatttcc agcgcggcgt
     7861 gctgcaacgc aacgccgacg gtgaacttga agtgacgacc accggacatc agggttcaca
     7921 tatatttagc tcctttagtc tcggcaactg ctttatcgtg ctggaacgcg atcgcggcaa
     7981 tgtggaagtg ggcgaatggg tggaagtaga accgtttaac gcgttgttcg gaggcctgta
     8041 atgacggaac tcagcgatca ggagatgctg cgctacaacc ggcaaatcat tctgcgcggt
     8101 tttgattttg acggtcagga ggcgctaaaa gattctcgtg tgctggtagt cggcctcggt
     8161 ggactcggct gcgcagcttc gcagtatctg gcaagcgccg gtgtcggcaa cctgacgctg
     8221 ctcgacttcg actcggtttc gctctcgaat ctgcaacgcc agacgctgca cagtgatgct
     8281 acggtcgggc aaccgaaggt agaatccgcc cgtgacgccc tgacgcggat caacccacat
     8341 atcgcgatta cgccagtcaa tgcactgctt gatgacgctg aacttgctgc gatgattgca
     8401 aaacacgatc tggtactcga ctgtacggat aacgttgcgg tgcgtaatca actgaacgct
     8461 ggctgttttg ccgcgaaggt gccgctggtt tccggcgcgg caattcgtat ggaagggcaa
     8521 atcacggtct tcacttatca ggacggcgaa ccgtgctatc gctgccttag ccgtttgttt
     8581 ggcgagaacg cattaacctg cgtagaagca ggcgtgatgg caccgttgat cggcgtaatt
     8641 ggttcgttgc aggcaatgga agcgatcaaa ctgctggcag gttatggcaa accggccagc
     8701 gggaaaatcg tcatgtacga tgcgatgacc tgtcagtttc gcgaaatgaa actgatgcgc
     8761 aatccggggt gtgaggtgtg cgggcagtaa ttgccacctc caggccagat aagacgcggc
     8821 actccgccac gtcatcagca atttatgagg atgcagaacg tccggttaaa tcgacgttct
     8881 gccaaacgct cccagccagt cctgttcaaa cttcgccaca gcggcttcca ccgccggata
     8941 gctaatcatc tgttgtgcca catccagtgg cagcgtaatt gattcacatc ctgccagtaa
     9001 gcagtccagc gcctgacgcg gggttttgaa actcgctgcc agcactttcg cctgcggcgc
     9061 atgcattttc aataactggt gtaagtcggt cacagtctga atgccgctac cgccctgagc
     9121 atcaatacga ttaacgtaag gcgcaacata ttccgcacct gccagcgccg acagcagccc
     9181 ttgtgctgcg ccatataccg cggttcccag cgttggaatc ccttccgctt ttaacatctt
     9241 aatagctgcc agcccctcgg cggtcaccgg aactttcacc acgatatccg caataataga
     9301 acgcaattta cgtgcgtcat tcaccatccc ttcagcagtg gtagccatta cctgggcaaa
     9361 cagacgcccc tgaccgccca tcgcttcatg aagttgcgga agcacaacat ccagcggttt
     9421 tttacccgcg gcgataatgc ttgggttagt ggtcacaccc gccagcggaa aaatacgtga
     9481 cagcgccttc accgcaacaa cgtctgaagt atccagatac agttccataa ccatcctcaa
     9541 aatgtacttt ttgtctgaat gtagactatc acgggaaaac tctctgaaca gttgacgcac
     9601 atcaatctaa ctttcattcg aaagtatttt aatgtttgaa cggaacagag aggcaactat
     9661 gattttcaat attcagcgct actcgaccca tgatggcccc ggtatccgca cggtcgtatt
     9721 tcttaaaggc tgttcgctgg gctgccgctg gtgtcagaac ccggaaagcc gcgcccgcac
     9781 gcaggatctg ctgtatgacg cacgactgtg tctggaaggc tgcgagctgt gcgctaaggc
     9841 cgcgccggaa gtgattgagc gcgcgctgaa tggtttgctt attcatcggg aaaagttaac
     9901 cccggagcat ctgacggcgt taaccgactg ctgtccgaca caggcattaa ccgtgtgtgg
     9961 tgaagtgaaa agcgttgagg agatcatgac gaccgttctg cgcgataaac cgttttacga
    10021 tcgcagcggc ggcggtttaa cgctttcggg tggtgagccc tttatgcagc cggaaatggc
    10081 gatggcgcta ctgcaagcca gccacgaggc aggcattcat actgcggtag aaacctgtct
    10141 gcatgtgccg tggaaatata tcgccccttc tctgccctat atcgatctgt ttcttgccga
    10201 tttaaaacac gttgccgacg cgccgtttaa acagtggacc gacggtaacg ccgccagagt
    10261 gctggataac ctgaaaaaac tcgccgcagc gggcaaaaaa atcattatcc gcgtgccgct
    10321 gattcagggc tttaatgccg acgaaacctc tgtaaaagcc attaccgatt ttgccgccga
    10381 cgagctgcac gttggcgaaa ttcattttct gccctaccac acgctgggca tcaacaaata
    10441 tcacttactt aatctgccct atgacgcccc ggaaaaaccg cttgatgcgc cagaactgct
    10501 cgactttgcc cagcagtatg cctgccagaa agggttaacc gcgaccttac gaggataaca
    10561 accatgacca cactgaaact ggacacgctc agcgaccgca ttaaagcgca caaaaatgcg
    10621 ctggtgcata ttgtgaaacc gccagtctgt accgagcgcg cgcagcacta taccgagatg
    10681 tatcaacaac atctcgataa gccgatcccg gtacgtcgcg cgctggcact ggcgcatcac
    10741 ctggcgaatc gcaccatctg gatcaaacac gatgagttga tcattggcaa ccaggcaagc
    10801 gaagttcgcg ccgcgccgat cttcccggaa tatactgtct cgtggatcga aaaagagatt
    10861 gatgatctgg cagatcgtcc cggtgctggc tttgcggtga gcgaagagaa caaacgcgtt
    10921 ctgcatgaag tgtgcccgtg gtggcgcggt cagaccgtac aggatcgctg ctacggcatg
    10981 tttaccgatg agcaaaaagg tctgctggcg accggaatca ttaaagcgga aggcaatatg
    11041 acctccggcg atgcacacct ggcggtgaat ttcccgctgc tgctggaaaa aggtcttgat
    11101 ggtctgcgtg agaaagtagc ggaacgtcgc tcgcgcatca atctgacggt gctggaagat
    11161 ttacatggcg aacaattcct gaaagcgatt gatatcgtgc tggtggcagt cagtgaacac
    11221 attgaacgtt tcgctgccct ggcgcgtgaa atggctgcta ccgaaactcg cgaaagccgt
    11281 cgcgatgaac tgctgacgat ggcagaaaac tgcgatctta tcgcccacca gccgccgcag
    11341 actttctggc aggcgctgca actgtgttac ttcatccaat tgattttgca gatcgaatct
    11401 aacggtcact cggtatcgtt tggtcgtatg gaccagtatc tctacccgta ctatcgccgc
    11461 gacgttgaac tcaaccagac gctggatcgc gaacacgcca tcgagatgct gcatagctgc
    11521 tggctgaaac tgctggaagt gaacaagatc cgctccggct cacactcaaa agcctctgcg
    11581 ggaagtccgc tgtatcagaa cgttaccatt ggcggacaaa atctggttga tggtcaacca
    11641 atggatgcag tgaatcccct ctcttacgcg atcctcgaat cctgcggtcg cctgcgttcc
    11701 actcagccta acctcagcgt gcgttaccat gcaggaatga gtaatgattt cctcgacgcc
    11761 tgcgtacagg tgatccgttg cggcttcggg atgccggcgt tcaacaacga cgaaatcgtg
    11821 atcccggaat ttattaaact cggtattgaa ccgcaggacg cttacgacta cgcagcgatt
    11881 ggttgtatcg aaaccgccgt cggtggcaaa tggggctatc gctgtaccgg catgagcttt
    11941 atcaacttcg cccgcgtgat gctggcggcg ctggaaggcg ggcgtgatgc caccagcggc
    12001 aaagtgttcc tgccacaaga aaaagcgttg tcggcaggca actttaacaa tttcgatgaa
    12061 gtaatggacg cgtgggatac gcaaatccgt tattacacgc gtaaatcaat cgaaatcgaa
    12121 tatgtcgtcg acaccatgct ggaagagaac gtacacgata ttctctgctc ggcgctggtg
    12181 gatgactgta ttgagcgagc gaaaagtatc aagcaaggcg gcgcgaaata tgactgggtt
    12241 tctggcctgc aggttggcat tgccaacctc ggcaacagcc tggcggcagt gaagaaactg
    12301 gtatttgaac agggcgcgat tggtcagcaa cagctggctg ccgcactggc ggatgacttc
    12361 gatggcctga ctcatgagca actgcgtcag cggctgatta acggtgcgcc gaagtacggc
    12421 aacgacgatg ataccgtcga tacgctgctg gctcgcgctt atcagaccta tatcgacgaa
    12481 ctgaaacagt accataaccc gcgctacggt cgtggtccgg ttggaggcaa ctattacgcg
    12541 ggtacctcgt ctatctccgc taacgtaccg tttggcgcgc aaactatggc aacaccggac
    12601 gggcgtaaag cccacacccc gctggcagaa ggcgcaagcc cggcctccgg tactgaccat
    12661 cttggcccta ctgcggtcat tggctcagtg ggtaaactgc ctacggcagc gattctcggc
    12721 ggcgtgttgc tcaaccagaa actgaatccg gcaacgctgg agaacgaatc tgacaagcag
    12781 aaactgatga tcctgctgcg taccttcttt gaagtgcata aaggctggca tattcagtac
    12841 aacatcgttt cccgcgaaac gctgctggag gcgaaaaaac atccggatca gtaccgcgat
    12901 ctggtagtgc gtgtcgcggg ctattccgcc ttcttcaccg cgctctctcc agacgcacag
    12961 gacgatatca tcgcccgtac tgaacatatg ctgtaatctc ctcggcccgg cggcgtcgtc
    13021 gccgggctaa atcattcaca tcatcaattt catccttact ttcattcgaa atataatttg
    13081 tgctctgcgt cacattgtta ttacactgtt acggtttcat tttcgagcca ggagcacacc
    13141 tatgagcgta aaagttatcg tcacagacat ggacggtact tttcttaacg acgccaaaac
    13201 gtacaaccaa ccacatttta tggcgcaata tcaggaactg aaaaagcgcg gcattaagtt
    13261 cgttgtcgcc agcggtaatc agtattacca gcttatttcg ttctttcctg agctaaagga
    13321 tgagatctct tttgtcgcgg aaaacggcgc actggtttac gaacatggca agcaactgtt
    13381 ccacggcgaa ctgacccgac atgaatcgcg gattgttatt ggcgagttgc taaaagataa
    13441 gcaactcaat tttgtcgcct gcggtctgca aagtgcatat gtcagcgaaa atgcccccga
    13501 agcatttgtc gcactgatgg caaaacacta ccatcgcctg aaagctgtaa aagattatca
    13561 ggagattgac gacgtactgt tcaagttttc gctcaacctg ccagatgaac aaatcccgtt
    13621 agtgatcgac aaactgcaca tagcgctcga tggcattatg aaacccgtca ccagtggttt
    13681 tggctttatc gacctgatta ttcccggtct acataaagca aacggtattt cgcggttact
    13741 gaaacgctgg gatctgtcac cgcaaaatgt ggtagcgatt ggcgacagcg gtaacgatgc
    13801 ggagatgctg aaaatggcgc gttattcctt tgcgatgggc aatgctgcgg aaaacattaa
    13861 acaaatcgcc cgttacgcta ccgatgataa taatcatgaa ggcgcgctga atgtgattca
    13921 ggcggtgctg gataacacat ccccttttaa cagctgacct ctctaccgga gccgtgcgct
    13981 ccggttttcc cttcttttta catcctgtac atcacatcaa atcattaaac ttgcattaac
    14041 ttatttataa cttatattat cacatgcaaa tcagaatggt ttcgaatgaa agttaatgag
    14101 aggtggttat ggcttctact tttaccagcg acacattgcc tgccgatcac aaagcagcta
    14161 tccgtcagat gaagcacgcg ctgcgggcgc agcttggcga cgtccagcag atctttaatc
    14221 agctaagcga tgacattgcc acgcgagtgg ctgaaatcaa cgcactcaaa gcacagggcg
    14281 atgccgtctg gccggtgctg tcttatgccg atatcaaagc aggtcatgtt actgcagagc
    14341 agcgcgaaca gattaaacgt cgcggttgtg cggtgataaa aggccatttc ccccgcgaac
    14401 aagcgctagg ctgggatcag tcgatgctgg actatctgga ccgcaaccgc tttgacgagg
    14461 tctacaaagg ccccggcgat aatttcttcg ggacgctcag cgcttcacgt cccgagattt
    14521 accccatcta ctggtcgcag gcgcaaatgc aggcccgcca gagtgaagaa atggcgaatg
    14581 cgcagtcgtt tctcaatcgt ctgtggacat ttgaaagtga tggaaagcaa tggtttaacc
    14641 cggatgtgag cgtcatctac cctgaccgta tccgccgccg tccgcccgga acgacctcca
    14701 aaggtcttgg agcgcatacc gactccgggg cgctggaacg ctggctgctt ccagcgtatc
    14761 agcgcgtttt cgccaacgtc tttaatggca atctggcgca atatgatccc tggcatgcgg
    14821 cacatcgtac ggaagttgaa gagtacacgg tggacaacac caccaaatgt tccgtgtttc
    14881 ggacattcca gggctggaca gcgctctctg atatgctgcc tggtcagggg ttgctgcacg
    14941 tcgtgcccat tcctgaagcc atggcgtacg tactgttacg tccgctgctt gaagatgtgc
    15001 cggaggatga actgtgcggc gtagcgcccg gaagagtgtt gccggtatca gagcaatggc
    15061 atccactgtt aattgaggcg ctaaccagca ttccaaaact cgaggccgga gactccgtct
    15121 ggtggcactg cgacgtcatc cattccgttg cccccgttga aaatcaacag ggttggggca
    15181 acgtgatgta cattcctgcg gcaccgatgt gcgagaaaaa tcttgcctac gcgcacaagg
    15241 tgaaggccgc actggaaaaa ggcgcatcgc cgggcgattt cccgcgcgag gactatgaaa
    15301 caaactggga aggacgcttt acgcttgccg acctcaacat tcacggtaag cgagcgttgg
    15361 gcatggatgt ttgagagtta atggaagtga attgatgtgg gattaagaaa atcgattcga
    15421 cgttactcag attgctgaca agtgcgcttt cttaatgcct gatgcggcgt taacgcctta
    15481 tctggcctac aaagtcctgc agattcaata tatcacaggt ac
//
LOCUS       DAZKIV010000109        15280 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZKIV010000109 DAZKIV010000000
VERSION     DAZKIV010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.109"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            268..1326
                     /gene="citC"
                     /locus_tag="ACHOT6_003319"
     CDS             268..1326
                     /gene="citC"
                     /locus_tag="ACHOT6_003319"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFP9948483.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAARCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLCYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1341..1637
                     /gene="citD"
                     /locus_tag="ACHOT6_003320"
     CDS             1341..1637
                     /gene="citD"
                     /locus_tag="ACHOT6_003320"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFP9948484.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1634..2542
                     /gene="citE"
                     /locus_tag="ACHOT6_003321"
     CDS             1634..2542
                     /gene="citE"
                     /locus_tag="ACHOT6_003321"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFP9948485.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALDAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2553..4085
                     /gene="citF"
                     /locus_tag="ACHOT6_003322"
     CDS             2553..4085
                     /gene="citF"
                     /locus_tag="ACHOT6_003322"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFP9948486.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4089..4640
                     /gene="citX"
                     /locus_tag="ACHOT6_003323"
     CDS             4089..4640
                     /gene="citX"
                     /locus_tag="ACHOT6_003323"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFP9948487.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4615..5493
                     /gene="citG"
                     /locus_tag="ACHOT6_003324"
     CDS             4615..5493
                     /gene="citG"
                     /locus_tag="ACHOT6_003324"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFP9948488.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVLNHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5544..7007
                     /gene="citT"
                     /locus_tag="ACHOT6_003325"
     CDS             5544..7007
                     /gene="citT"
                     /locus_tag="ACHOT6_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFP9948489.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRALTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7148..7927
                     /gene="rna"
                     /locus_tag="ACHOT6_003326"
     CDS             7148..7927
                     /gene="rna"
                     /locus_tag="ACHOT6_003326"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFP9948490.1"
                     /translation="MLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQTGFCQSQHD
                     RNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFGCATRPIPNL
                     PEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACFGFDPDAYFG
                     TMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKLTCQGNPAYL
                     TEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8157..8567
                     /gene="rnk"
                     /locus_tag="ACHOT6_003327"
     CDS             8157..8567
                     /gene="rnk"
                     /locus_tag="ACHOT6_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFP9948491.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8744..8794
                     /locus_tag="ACHOT6_003328"
     CDS             8744..8794
                     /locus_tag="ACHOT6_003328"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948492.1"
                     /translation="MAAIIVMAVLYLENHS"
     gene            complement(8773..10035)
                     /gene="ybdR"
                     /locus_tag="ACHOT6_003329"
     CDS             complement(8773..10035)
                     /gene="ybdR"
                     /locus_tag="ACHOT6_003329"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HFP9948493.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVIVVPGAQS
                     AEAAQKAVSGLVNAMPGEQYDRQEWFSR"
     gene            10256..10684
                     /gene="uspG"
                     /locus_tag="ACHOT6_003330"
     CDS             10256..10684
                     /gene="uspG"
                     /locus_tag="ACHOT6_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFP9948494.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10805..12370)
                     /gene="ahpF"
                     /locus_tag="ACHOT6_003331"
     CDS             complement(10805..12370)
                     /gene="ahpF"
                     /locus_tag="ACHOT6_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFP9948495.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DKYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12541..13104)
                     /gene="ahpC"
                     /locus_tag="ACHOT6_003332"
     CDS             complement(12541..13104)
                     /gene="ahpC"
                     /locus_tag="ACHOT6_003332"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFP9948496.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13476..14222
                     /gene="dsbG"
                     /locus_tag="ACHOT6_003333"
     CDS             13476..14222
                     /gene="dsbG"
                     /locus_tag="ACHOT6_003333"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFP9948497.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWIDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14431..>15280
                     /gene="ybdO"
                     /locus_tag="ACHOT6_003334"
     CDS             14431..>15280
                     /gene="ybdO"
                     /locus_tag="ACHOT6_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9948498.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSS"
BASE COUNT         3750 a         3975 c         3740 g         3815 t
ORIGIN      
        1 aaatcactta aacctgcagg tcatttcgta attacattaa ttaaaaaact taaaaccatt
       61 aaataaataa aaaaccacta actctatgtg aaataaatca aaatttcacg ctgcaatact
      121 ccttaggatg tatagcgaaa ggagaaaaag atatacctcg atcacccccc tttctcccaa
      181 gtgaaaataa aaggttatca gtttgcaaca ttgaacaaca ttcgttgcaa atcgataaca
      241 acatgtacct tcaggatact atttattatg ttcggcaatg atattttcac ccgcgtaaaa
      301 cgttcagaaa ataaaaaaat ggcggaaatc gcccaattcc tgcatgaaaa tgatttgagc
      361 gttgacacca cagtcgaagt atttattacc gtaacccgcg atgaaaagct tatcgcgtgc
      421 ggtggaattg ccggaaatat tattaaatgc gttgctatca gtgaatccgt tcgcggtgaa
      481 ggactggcgc tgacattagc cactgaattg ataaacctcg cctatgagcg gcacagcacg
      541 catctgttta tttataccaa aaccgaatac gaggcgctgt tccgccagtg cggtttttcc
      601 acgctgacca gcgtacccgg cgtgatggtg ctgatggaaa acagcgccac gcgactgaaa
      661 cgctatgccg aatcgctgaa aaaatttcgt catccaggga acaagattgg ctgcattgtg
      721 atgaacgcca atccctttac gaatggtcac cgttatctga ttcaacaggc tgcggcacgg
      781 tgtgactggt tgcatctgtt tttagtcaaa gaagattctt cacgcttccc ctatgaagac
      841 cggctggatc tggtgttaaa aggcaccgcc gatattccac gcctgactgt gcatcgcggc
      901 tccgaataca tcatctcccg cgctacgttc ccttgctact tcattaaaga acagagcgtc
      961 attaaccatt gttacaccga aattgatctg aagattttcc gtcagtacct cgctcccgcg
     1021 ctgggtgtaa ctcaccgctt tgtcggtact gaaccctttt gtcgcgttac cgcccagtac
     1081 aaccaggata tgcgctactg gctggaaacg ccgactatct ccgcaccgcc catcgaactg
     1141 gttgaaattg agcggctgtg ttaccaggag atgccgatat ccgcttcccg ggtacgtcaa
     1201 ctgctggcga aaaacgatct cacggctatc gcgccgctgg tccctgcagt cacgctgcat
     1261 tatttgcaga acctgcttga gcactcccgc caggacgcgg cagctcgtca aaagaccccc
     1321 gcatgagaaa caggtgaaaa atgaaaataa accagcccgc cgttgcaggc acccttgagt
     1381 ctggggatgt gatgatacgc atcgccccac tcgatacgca ggatatcgac ctgcaaatca
     1441 atagcagcgt tgagaaacag tttggcgatg caattcgcac caccattctg gacgttctcg
     1501 cccgctacaa cgtgcgcggc gtacagctga atgtcgatga caaaggcgca ctggactgca
     1561 ttttacgtgc acgactggaa gccctgctgg cacgcgccag cggtatcccg gctctgccat
     1621 gggaggattg ccaatgattt ccgcttcgct gcaacaacgt aaaactcgca cccgccgcag
     1681 catgttgttt gtgcctggtg ccaatgccgc gatggtcagc aactccttca tctacccggc
     1741 tgatgccctg atgtttgacc tcgaagactc cgtggcatta cgtgaaaaag acaccgcccg
     1801 ccgcatggtt tatcacgcgc tgcaacatcc gctgtatcgc gatattgaaa ccattgtgcg
     1861 tgtaaacgcg ctggattccg aatggggtgt caacgacctg gaagccgtcg ttcgcggtgg
     1921 tgcggacgtt gtgcgtctgc cgaaaaccga taccgctcag gatgttctgg atatcgaaaa
     1981 agagatcctg cgtatcgaaa aagcctgtgg tcgtgaaccc ggcagcaccg gcctgctggc
     2041 ggcgattgaa tctccgctgg gcattacccg cgcagtggaa atcgctcacg cttccgagcg
     2101 tttgatcggt atcgccctcg atgcagaaga ctatgtgcgc aacctgcgta cagaacgctc
     2161 cccggaagga actgaactgc tgttcgcacg ctgttccatt ttgcaggccg cgcgctctgc
     2221 gggtattcag gcgttcgata ccgtctattc cgacgctaac aacgaagccg gattcctgca
     2281 agaagccgcc cacatcaaac agctgggctt tgacggcaaa tcgctgatca acccgcgtca
     2341 gattgatctg ttgcacaacc tctacgcacc aacccagaaa gaagtggatc acgcccgccg
     2401 cgtcgtagaa gccgctgaag ccgccgctcg cgaaggcctc ggcgtggttt ccctgaacgg
     2461 caagatggtg gacggtccgg ttatcgatcg cgcccgtctg gtgctctctc gtgcagaact
     2521 ttccggcatc cgcgaagaat aaggcaatca aaatgacgca gaaaattgaa caatctcaac
     2581 gacaagaacg ggtagcggcc tggaatcgtc gcgctgaatg cgatcttgcc gctttccaga
     2641 actcgccaaa gcaaacctac caagctgaaa aagcgcgcga tcgcaaactg tgcgccaacc
     2701 tggaagaagc gattcgtcgc tctggtttac aggacggcat gacggtttcc ttccatcacg
     2761 ctttccgtgg cggtgacctg accgtcaata tggtgatgga cgtcatcgcg aagatgggct
     2821 ttaaaaacct gaccctggcg tccagctccc tgagtgattg ccatgcgccg ttggtagaac
     2881 acattcgcca gggcgtggtt acccgcattt atacctccgg cctgcgcggt ccactggcgg
     2941 aagagatctc ccgtggtctg ctggcggaac cggtgcagat ccactctcac ggcggtcgtg
     3001 tgcatctggt acagagcggc gaactgaata tcgacgtggc tttcctcggc gtcccgtcct
     3061 gtgatgaatt cggtaatgcc aacggctaca ccggtaaagc ctgctgcggc tccctcggct
     3121 atgcaatggt tgatgccgac aacgcaaaac aggttgtgat gcttaccgaa gaactgctgc
     3181 cttatccgca taatccggca agcattgagc aagatcaggt tgatttaatc gtcaaagttg
     3241 atcgcgttgg cgatgctgca aaaatcggcg ctggtgctac ccgtatgacc actaacccgc
     3301 gcgaactgct tattgcccgt agcgctgcgg atgtgattgt caactcaggc tacttcaaag
     3361 aaggtttctc catgcaaacc ggcaccggcg gcgcatcgct ggcggtaacc cgtttcctgg
     3421 aagacaaaat gcgtagccgc gatattcgcg ccgacttcgc ccttggcggt attaccgcga
     3481 cgatggttga cctgcacgaa aaaggtctga tccgcaaact gctggatgtg cagagctttg
     3541 acagccatgc tgcgcaatcg ctggcccgta accccaatca catcgaaatc agcgccaacc
     3601 agtacgctaa ctggggttcg aaaggcgcat cggttgatcg tctcgacgtg gtggtactga
     3661 gcgcgctgga aattgacacc cagttcaacg ttaacgtgct gaccggctct gacggcgtac
     3721 tgcgtggtgc ttccggtggt cactgcgata ccgcgattgc ctctgcgctt tccatcatcg
     3781 tcgcgccgct tgtacgcggt cgtattccga ctctggtgga taacgtgctg acctgcatca
     3841 ccccaggctc cagtgtcgat attctggtca cagaccacgg tatcgcagtt aacccggcac
     3901 gtccggaact ggcagaacgt ctgcaggaag cgggcattaa agtggtttcc attgagtggc
     3961 tgcgcgaacg cgcgcgtctg ctgaccggtg aaccacagcc gattgaattc accgaccgcg
     4021 tcgttgccgt tgtgcgttac cgcgatggct cggtgatcga tgttgtgcat caggtgaagg
     4081 aataagccat gcacctgctt cctgaactcg ccagccacca tgcggtgtca attcccgagc
     4141 tgctcgtcag ccgggatgaa aggcaagcac ggcaacacgt ctggctcaag cgccatcctg
     4201 ttccactggt ctcctttacc gtggttgcgc ctgggccgat taaagacagc gaggtcacac
     4261 gccgaatttt taatcatggc gtgacagcct tgcgtgcctt agccgcaaaa cagggctggc
     4321 aaattcagga gcaggctgca ctggtttccg ccagcgggcc ggagggcatg ttgagcattg
     4381 ccgccccggc tcgcgacctc aagctcgcca ccattgagct tgaacatagt catcctctcg
     4441 ggcggttatg ggatatcgat gtcctgacgc ccgaaggcga aattctctcc cgccgcgact
     4501 attcactgcc gcctcgccgc tgcctgttgt gcgagcaaag cgcagccgtc tgcgcgcgtg
     4561 gaaaaaccca tcaactgacc gatttactca accgcatgga ggcactgctg aacgatgtcg
     4621 atgcctgcaa cgtcaactaa aaccacaaag cttgcgacgt cattaatcga tgagtacgcc
     4681 ctgctgggct ggcgcgccat gctgactgaa gtcaatctgt caccgaaacc aggcctcgtg
     4741 gatcgcatta actgcggtgc gcacaaagat atggcgctgg aagatttcca ccgcagcgcg
     4801 ctggcgattc agggctggtt accccgtttc attgaatttg gtgcctgtag tgcagaaatg
     4861 gcaccggaag cggtactcca cggattacgc ccaattggta tggcttgcga aggtgatatg
     4921 ttccgcgcca ctgcgggcgt aaacacgcat aaaggcagca ttttttcttt agggctgcta
     4981 tgtgcggcaa ttggccgttt gcttcagctc aaccagccgg taacgccaac aaccgtttgt
     5041 tctacggcgg caagtttctg ccgtggtctg accgatcgcg aactgcgtac caataattca
     5101 caactgacgg caggtcaacg gttgtaccag cagcttggcc ttaccggcgc acgcggtgaa
     5161 gccgaagcgg gttatccact agtgctcaac cacgccctgc cgcattacct cactctgctg
     5221 gatcaggggt tagatcctga actggcattg ctcgatacct tgctcctact gatggcgatc
     5281 aacggcgata ccaacgttgc gtcgcgcggt ggcgaggggg gcctgcgctg gctacagcgc
     5341 gaggcgcaaa cattattgca aaaagggggc attcgaaccc ccgccgatct cgattatctc
     5401 cggcagttcg acagggagtg tatcgaacga aatctcagtc caggcggcag tgccgaccta
     5461 ctgatcctta cctggttttt agcacagatt taattattta agtacttgat aaatttggaa
     5521 atattaattt tcggagaacc cgtatgtctt tagcaaaaga taatatatgg aaactattgg
     5581 ccccactggt ggtgatgggt gtcatgtttc ttatccctgt ccccgacggt atgccgccgc
     5641 aggcatggca ttacttcgct gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa
     5701 ttccggcaac agcgatcagt tttattgcgg ttactatttg cgttattggc agtaattacc
     5761 tgctctttga tgccaaagaa ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga
     5821 aatggggtct ggctggtttt tccagcacca cggtatggct ggtatttggc gcatttattt
     5881 ttgcattagg gtatgaagtt tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat
     5941 tcatgggcaa acgcgcgctg acgttgggtt atgcgattgt cattatcgac attctgctgg
     6001 caccgtttac accgtccaac accgcgcgta ccgggggtac ggtttttccg gtcattaaaa
     6061 acctgccgcc gttatttaaa tcattcccca acgatccgtc cgcgcgtcgt attggcggct
     6121 atttgatgtg gatgatggtc attagtacca gtctgagttc gtccatgttt gtcaccggtg
     6181 cggcaccaaa cgtgctgggt ctggagttcg tcagcaaaat tgccggtatc cagattagct
     6241 ggttgcagtg gttcctctgc ttcctgccgg ttggggttat cttgcttatc attgcgccgt
     6301 ggctttccta cgtgctgtac aaaccggaaa tcacacacag tgaagaagtg gcaacctggg
     6361 cgggtgatga actgaaaacc atgggtgcgc tgacacgcag agagtggaca ctgattggcc
     6421 ttgtattgct cagcttaggt ttgtgggttt ttggcagtga agtcattaat gctactgcag
     6481 ttggtctgct ggcagtttcg ctaatgctgg ccctgcacgt tgtgccttgg aaagatatta
     6541 cccgctataa cagcgcatgg aacacgctgg tcaacctggc aactctggtt gtgatggcta
     6601 acggcctgac tcgttctggc tttattgact ggttcgccgg taccatgagt acgcacctgg
     6661 aaggattctc accaaacgca acggtgattg tactggttct ggtgttctac tttgcacact
     6721 acctgtttgc cagcctgtct gcgcacaccg caaccatgct gccggttatt ctggccgtcg
     6781 gtaaaggtat tccgggcgta ccaatggaac aactgtgtat cctgctggtg ctgtctatcg
     6841 gtatcatggg ctgtctgacg ccgtatgcaa ccggtcctgg ggtgattatt tacggctgtg
     6901 gctatgtgaa atcaaaagat tactggcgtc ttggcgcaat cttcggggtg atttacatct
     6961 ctatgttgct gttggttggc tggccgattc tcgccatgtg gaactaatac gttcgccaaa
     7021 cgctgaataa atcatcacgc ccgccaggta agcccacctg gcgggctttt ttatgattta
     7081 atagatagta ttcagtgtat gtatgagttc cacacccatt atgaaagcat tctagcgtaa
     7141 tgccgcgttg ctcgcggttt ctctgcttcc cttctcttct gccaacgccg tagcgttgca
     7201 ggcaaaacag tatggcgatt ttgatcgcta tgtcctggcc ctctcctggc aaaccggatt
     7261 ttgccagagt caacacgatc gaaatcgtaa cgaacgagat gaatgtcgcc tgcaaaccga
     7321 aacgaccaac aaagctgatt ttctgaccgt acatggtctg tggccaggat tgcctaaatc
     7381 ggttgctgcc cgtggtgttg atgaacgccg ctggatgcgc ttcggttgcg ctactcgccc
     7441 aatcccgaat ctaccagaag cgcacgccag ccggatgtgt tcatcgccgg aaaccggatt
     7501 atcactggaa acggccgcta aactaagtga agtcatgcca ggagctggcg gacgttcctg
     7561 cctggaacgc tacgaatatg ccaaacacgg tgcctgcttt ggttttgatc cggacgcata
     7621 cttcggtacg atggtacgcc tgaatcaaga aattaaagag agcgaagccg gaaaattcct
     7681 tgcggataat tacggtaaaa cagtgagccg ccgtgacttt gacgccgcct ttgccaaaag
     7741 ctggggaaaa gagaacgtga aagcagttaa gctaacgtgc cagggtaacc ctgcgtattt
     7801 gactgaaatt cagatctcga tcaaagctga cgccatcaac gctccgcttt ctgcaaactc
     7861 attcttgcca caaccacacc caggcaactg tggcaaaacc tttgtgattg ataaagtggg
     7921 ttattaatca tctgatttac aaaacacgct aattcgtctt ccataaaccg tgacgattaa
     7981 gaattataca taatgcgctg cattgaggaa ttccctgaat gtgacgcaaa tcacttcaaa
     8041 gagtgagcaa cagtcagtat catcatgtga gttaaaccct cgccgcctga cggtgagggt
     8101 tttcttttgg gtatgtttat ctgcgacact cgcagtaccg acgacatgga gtaaaaatgt
     8161 ccagaccaac tatcatcatt aacgacctgg atgccgaacg catcgatatt ctgctggagc
     8221 aacccgccta tgctggtttg ccaatcgccg acgcgttaaa cgcagagttg gatcgcgccc
     8281 aaatgtgttc gccagaagag atgccacacg acgtggtgac aatgaacagc cgggttaaat
     8341 tccgcaatct tagcgatggc gaagtgcgtg tgcgcacgct ggtgtatccg gcaaaaatga
     8401 ccgatagcaa tactcagctt tccgttatgg ctccggtagg tgccgcactg ctggggctgc
     8461 gcgttggcga ttccattcac tgggaacttc cgggcggcgt tgcaacccac cttgaagtgc
     8521 tggaactcga ataccagcca gaagctgctg gcgactacct gctttaatcc catctgcaca
     8581 agcgataact cgctttgccc agaggatgca ctcgcatcct ctttacgcaa aatcagcaat
     8641 ttttcctggt tctcactgtt cacttttgct gcaaaattat tccgtttcgt ttgtgggaat
     8701 aaccatggca gaagcatttt acatcctgat aggatttctg attatggcgg cgattatcgt
     8761 catggctgtc ctttacctcg aaaaccactc ctgacgatca tattgttccc ccggcatcgc
     8821 attcaccaga cctgaaaccg ccttctgcgc cgcctctgcg ctttgtgcac cgggtaccac
     8881 aatcacctta cggcactcct cttcacgttt ttcgaaaatc tcatatcccc gggcggcctc
     8941 ttcaaacggc atatagtggg taacaatttc ttctggtttc agtaatcctt tctcaattaa
     9001 cggtaataat tctcccagcc atgcgtgaac gtgggtctgt cccattttaa acgtcaaccc
     9061 tttatcaaag gcgtcgccaa acaggaaacc gtgaataaat ccagcgtaga cgcccggtac
     9121 gctaacaatg ccgccacgcc tgaccgccgc aatacactgg cgcaacgctt taccgctgct
     9181 gccctccagt ttcaggttag tcagcaccgt ttccgtggtg ctgcctttcg cttcaaaacc
     9241 gacggcgtct attactgcat ccacgccccg gtgacctgcc gtttgttcaa taattgactg
     9301 tgccggatcg ctatcttcat cgaaattaat cgggatcgcg ccgtagcggt cagcggcgaa
     9361 actcaagcgg tagggatgat gatcaacaac aaaaatctgt tccgcaccga gcaaccgtgc
     9421 acaggcgatt gtcaacaatc ccactggacc ggcaccatag actgcaacgc ttgaaccttg
     9481 ttggatctgc gcattttttg ctgcctgcca tgccgttggc agaatatcag aaaggaaaag
     9541 ggctttatca tctgaaagca aaggcggtac tttaaacggc cccacattcc ctttagggac
     9601 gcggacatat tccgcctgtc caccaggaac gccaccatac aggtgactat aaccaaacaa
     9661 tgccgcggga gctggtatct gttttttatt gagcgcagcg cctttacccg cattggtatt
     9721 ttcgcaggcg gcatattgct gcattcgaca gaaaaaacag tcgccacaag caatgacaaa
     9781 cggaattacc acccggtcgc ctttttgcaa attttttacg tcctttccgg tttcaacgac
     9841 ttcccccata aattcatgac caaaaatatc gccatgttta acctggggta ttttgcctcg
     9901 ataaagatgg aggtcagagc cacagatcgc cgttgccgta atacgcagaa taatatcatc
     9961 tgcctgttca atgcccggat cgggaacatt ttctacctga acgtgatgtg ggccgtgata
    10021 agtcaatgct ttcatgcaac ctccagagac agttcaaaaa agagtcagtc tttaagggta
    10081 gctatcacag cgggaaaacg ggcggtaccg caggtttttg aagaattcct ggtgttcagt
    10141 gagaatatat taatcacaga aaccgttcgt aaggaaaaag aaccagacaa atatcaggga
    10201 taaatattat aatcatggcg aatgatttgt tcatgattaa cagggagaaa gggttatgta
    10261 taagacaatc attatgccag ttgatgtatt tgaaatggaa ttgagcgaca aagctgttcg
    10321 ccacgctgaa ttcctcgccc aggatgacgg agttattcat ctacttcacg tactacccgg
    10381 gtcagccagc ctgagcctgc accgttttgc cgctgatgtg cgtcgttttg aagagcatct
    10441 gcaacatgaa gcagaagaac gtctgcaaac gatggtcagc cacttcacca tcgatccttc
    10501 ccgcattaaa caacatgtcc gttttggtag cgtgcgggat gaagtcaatg agttggcaga
    10561 agaactgggg gctgatgttg tagttattgg ttctcgcaac ccatcgattt cgacccatct
    10621 gttaggttct aacgcctcga gcgtgatccg ccatgccaat ctgccggtgc tggttgtgcg
    10681 ttaattgcca gaataaatat cccgccctgc ccggagccat cccggcaggg ctttttaatg
    10741 ccataaaaaa gccgccaggt ttgaccccgg cggctaagca attgcaggtg aatcttactt
    10801 cttcttatgc agttttggtg cgaatcaggt agtcaaaagc actcagagag gctttggcac
    10861 cttcgccagt ggcgatgatg atctgcttgt acggaaccgt cgtacagtca cccgctgcga
    10921 atacgccttt gacgttggtt tcgcatttcg catcaatgat aatctcaccc atgcggttac
    10981 gttcgactgc gccttccagc cagttggtgt tcggcagcag accaatctgg acgaaaatac
    11041 cggccagttc gatgttgtga atatcgccgc tgacacgatc tcgatattcc agaccaacga
    11101 ctttgctgcc gtcgcctttc acttccgtgg tttgcgcatt cagaataatg tcgacgtttt
    11161 tcaggctgcg cagtttgtcc tgcagaacct ggtcggcttt catttctggc gcaaattcca
    11221 gcagcgttac gtgctcaacg atacccgcca ggtcaattgc cgcttccacg ccggagttac
    11281 cgccgccgat aaccgctacg cgtttacctt taaacagcgg gccgtcgcag tgcgggcagt
    11341 aggtcacgcc tttggtgcga tacttatctt cgcccggaac gttcatgttg cgccattttg
    11401 caccggtcgc cacgataatg ctgcgtgctt tcagtaccgc gccagaagct gtttcaatct
    11461 gatgcagacc accttcaact gctgctggga tcagtttgct ggcgctctgg ctgtcgatca
    11521 catcaacgtc gtattcatca acgtgaactt tcagtgcgcc tgccagcttc tgcccttcag
    11581 tcttcggtac agaaatgtag ttttcgatat caacggtatc gaggatctga ccaccaaaac
    11641 gttcgcccat cagaccggta cggatgcctt tacgtgcgga gtaaattgct gccgctgcac
    11701 ccgccgggcc ggaaccaacg attaatacgt cataagcatc acgcttgttc agctcttctg
    11761 ccgcacgttt ttccgcgccc gtatcaattt tggcaacgat ttcagtcaac gtcatgcggc
    11821 cctgaccaaa ctctttcccg tttacgaaca ctgccggaac gcccatcacg ttgcgatcgg
    11881 tgatttcgtt ctggaaggtg ccgccgtcaa ttgcagtgtg cttgatgcgc gggttcagta
    11941 cgctcatcag gttcagcgcc tgcaccacgt ccgggcagtt gtggcaagag agcgagtaat
    12001 aggtttcgaa ttcaaaatca ccgtcaatat ggcgaatctg ctccagcaga gactgcgctt
    12061 ctttcgacgg atgaccaccg gtccacagca acgccagtac cagcgaggtg aactcgtggc
    12121 ccagcgggga gcctgcaaaa cgtggcccct ggttggaacc tgggttggtg atcaggaaag
    12181 acggcttacg caccggcaag ctgttatctt ctttaaaggt gactttgtct gacagttctg
    12241 cgatttcagc caacagttcc ttgatttctg ccgatttagc gctgtcatcc agcgtggcaa
    12301 ttaactcaac aggcttggtc aatttctcaa ggtaagcctt gagttgagtt ttcatatttg
    12361 tgtcgagcat gtttatctcc tgggctttaa acatcatcat gcaagcggcc tctgcggcta
    12421 cctggatgca gcttgcatca tggtgcgggg aagaaaatcg ggtgccgcag cacccgaaga
    12481 attaggtgaa tttccgaagt atttcgcgcc ggagttggta gcgcgaaaga cgaaggaaat
    12541 ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt ctttccattt
    12601 agccgggcaa acttcacctg ggtgagaagc tacgtactgt gctgctttga ttttacgcag
    12661 caggtcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct ggatgatacc
    12721 ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac gcatgttgtc
    12781 gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga ttttagcgat
    12841 ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag atactgcgta
    12901 tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac ccagttcggt
    12961 cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc ggccttcggt
    13021 atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt taaaaggttt
    13081 aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga gatgtaaggt
    13141 aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa gttttacctt
    13201 acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa aaagtaaaat
    13261 ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc agcgcctgcc
    13321 tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa caccctcata
    13381 aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt tatgcctttt
    13441 actcgacttt tgcactgact gaaaaggaca aattaatgtt aaaaaagata cttttactgg
    13501 ctctgcttcc tgcaatcgcc ttcgcagagg aacttcctgc tccagtaaaa gcgattgaaa
    13561 aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa ggttatctcg
    13621 gaaagtatca ggatatgggc gtcaccatct acctgactcc agatggtaag cacgctatct
    13681 ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc gaaaaagaaa
    13741 tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac tggctcctcg
    13801 acggtaaaaa agatgcgccg gtcattgtct acgtgttcgc cgatccgttc tgcccatatt
    13861 gtaaacagtt ctggcagcag gcgcgcccgt ggatagattc tggcaaagtg caattaagaa
    13921 cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca attcttgcct
    13981 ccaaagatcc cgcaaaaacc tggcaagaat atgaatcctc tggtggcaag cttaagctaa
    14041 acgtgcctgc aaacgtaagt acagaacaaa tgaaagtgtt aagcgccaac gagaaactca
    14101 tggacgatct gggggcaaat gtcacaccag ctatctatta catgagtaag gaaaatacgc
    14161 tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg gggaataaat
    14221 aaaagataat tcatggatgg ttgaaataca tccctgcttt tcaaccatcc taaaaaagca
    14281 tgaattgctc gctctgccct aagtggctat gctattttag aaaaacttaa tcctgagtta
    14341 agcaaaaaaa tataatcaat aaaaatatat acgcaaaaaa cattgattaa gtgaatatac
    14401 catggaagaa aaatataacc ggagtagtgt atggccaatc tctacgactt gaaaaagttc
    14461 gacttaaacc ttcttgtcat atttgagtgt atttaccagc atttaagcat tagcaaagct
    14521 gctgaatcac tatatatcac cccatcagcc gtgagccagt cgttacaacg cttacgtgcg
    14581 caatttaatg accctttatt cattcgctca ggaaaaggta tcgcgccgac gacaacaggg
    14641 ttgaatctgc atcaccatct tgagaaaaac ctcaaaggcc ttgaacaaac aattaatata
    14701 gtcaataagt ccgaactaaa gaaaaacttt attatctacg gccctcagct catttcttgc
    14761 tctaataata gtatgctgat ccgctgccta cggcaagatg cctctgtcga gattgagtgc
    14821 catgatatcc tcatgtcagc agagaatgct gaagaactgt tggttcaccg caaagccgat
    14881 ctggtgatca cccaaatgcc agtgatcagc cgttccgtca tttgcatgcc attgcataca
    14941 attcgcaata ctctaatttg tagtaataag catccgcgaa ttactgacaa tagcacttat
    15001 gaacaaatta tggcagaaga gttcacccaa ttaatatcca aatcagcagg agttgatgat
    15061 attcaaatgg aaatagacga gaagtttatg aatcgcaaaa tttcatttcg tggttcctct
    15121 ttgctgacaa tcattaatag cattgcagtt acagatttac tgggaatagt cccctatgaa
    15181 ctttataatt cctaccgtga ctttcttaat ttaaaagaga taaaactaga acatccgtta
    15241 ccttcaataa agctttacat ttcctataat aaatcttctt
//
LOCUS       DAZKIV010000110        15240 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZKIV010000110 DAZKIV010000000
VERSION     DAZKIV010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.110"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..605
                     /gene="yceJ"
                     /locus_tag="ACHOT6_003335"
     CDS             39..605
                     /gene="yceJ"
                     /locus_tag="ACHOT6_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9948499.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            609..1184
                     /gene="yceI"
                     /locus_tag="ACHOT6_003336"
     CDS             609..1184
                     /gene="yceI"
                     /locus_tag="ACHOT6_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFP9948500.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1226..2278)
                     /gene="trhO"
                     /locus_tag="ACHOT6_003337"
     CDS             complement(1226..2278)
                     /gene="trhO"
                     /locus_tag="ACHOT6_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFP9948501.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2503..3423
                     /gene="lpxL"
                     /locus_tag="ACHOT6_003338"
     CDS             2503..3423
                     /gene="lpxL"
                     /locus_tag="ACHOT6_003338"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFP9948502.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3595..4821
                     /gene="mdtG"
                     /locus_tag="ACHOT6_003339"
     CDS             3595..4821
                     /gene="mdtG"
                     /locus_tag="ACHOT6_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFP9948503.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4904..5278
                     /gene="msyB"
                     /locus_tag="ACHOT6_003340"
     CDS             4904..5278
                     /gene="msyB"
                     /locus_tag="ACHOT6_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFP9948504.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5279..5506)
                     /gene="yceK"
                     /locus_tag="ACHOT6_003341"
     CDS             complement(5279..5506)
                     /gene="yceK"
                     /locus_tag="ACHOT6_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFP9948505.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5679..8222)
                     /gene="mdoH"
                     /locus_tag="ACHOT6_003342"
     CDS             complement(5679..8222)
                     /gene="mdoH"
                     /locus_tag="ACHOT6_003342"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFP9948506.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8215..9750)
                     /gene="mdoG"
                     /locus_tag="ACHOT6_003343"
     CDS             complement(8215..9750)
                     /gene="mdoG"
                     /locus_tag="ACHOT6_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFP9948507.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYETPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10144..11301
                     /gene="mdoC"
                     /locus_tag="ACHOT6_003344"
     CDS             10144..11301
                     /gene="mdoC"
                     /locus_tag="ACHOT6_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFP9948508.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMLLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(11309..12790)
                     /gene="clsC"
                     /locus_tag="ACHOT6_003345"
     CDS             complement(11309..12790)
                     /gene="clsC"
                     /locus_tag="ACHOT6_003345"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFP9948509.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(12732..13265)
                     /gene="ymdB"
                     /locus_tag="ACHOT6_003346"
     CDS             complement(12732..13265)
                     /gene="ymdB"
                     /locus_tag="ACHOT6_003346"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFP9948510.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(13360..13671)
                     /gene="ymdA"
                     /locus_tag="ACHOT6_003347"
     CDS             complement(13360..13671)
                     /gene="ymdA"
                     /locus_tag="ACHOT6_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFP9948511.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIEY
                     N"
     gene            complement(13792..14124)
                     /gene="csgC"
                     /locus_tag="ACHOT6_003348"
     CDS             complement(13792..14124)
                     /gene="csgC"
                     /locus_tag="ACHOT6_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFP9948512.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPDDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(14183..14638)
                     /gene="csgA"
                     /locus_tag="ACHOT6_003349"
     CDS             complement(14183..14638)
                     /gene="csgA"
                     /locus_tag="ACHOT6_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFP9948513.1"
                     /translation="MKLLKVAAIAAIVFSCSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(14679..15134)
                     /gene="csgB"
                     /locus_tag="ACHOT6_003350"
     CDS             complement(14679..15134)
                     /gene="csgB"
                     /locus_tag="ACHOT6_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFP9948514.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         3792 a         3921 c         3638 g         3889 t
ORIGIN      
        1 cattttcttt ctctcgcaaa ttcgtattgg atgcacttat gtcattcaca aatacccctg
       61 aacgctatgg tgttatatct gcggccttcc attggctaag cgcaattatt gtctacggca
      121 tgtttgcttt aggtctgtgg atggtcacgc tcagttatta cgatggctgg tatcacaaag
      181 cacccgaact gcataaaagc atcggtattt tgttaatgat ggggctggtt attcgcgttc
      241 tgtggcgtgt catatctcca ccgccgggtc cgctgccgag ttattcgccc atgactcgtc
      301 ttgccgcaaa ggctggacat ctcgcattgt acctattgct gttcgccatc ggcatcagcg
      361 gctatctgat ctcaactgcc gatggtaaac cgatcagcgt ttttggctgg tttgacgtcc
      421 ccgcgaccct tgccgacgct ggcgcacagg cagactttgc cggtgccctg catttttggc
      481 tcgcgtggag cgtcgtagta ctgtccgtta tgcacggatt tatggccctg aagcatcatt
      541 tcatcgataa agacgacact ctgaagcgca tgctgggaaa gtcgtcatct gactatggag
      601 tataaaaaat gaaaaaaagc ctgcttggtt taaccttcgc gtccctgatg ttctctgccg
      661 gttcagcggt tgccgccgat tacaaaattg acaaagaagg tcagcacgcc tttgttaatt
      721 tccgcatcca gcaccttggc tatagctggt tatacggcac ctttaaagat ttcgacggta
      781 cttttacctt tgacgaaaaa aatccggctg ccgataaagt gaatgtgaca attaacacca
      841 ccagcgtcga tactaatcac gccgaacgcg ataaacatct tcgcagtgca gatttcctca
      901 ataccgcaaa atatccacag gcaacattca cctccaccag cgtgaagaaa gacggtgacg
      961 aactggatat taccggcgat ctgacgctga atggcgtaac caaacccgtc acgctggaag
     1021 cgaaattaat tggtcagggc gacgacccat ggggtggtaa acgtgcaggc ttcgaggccg
     1081 aaggcaaaat taagctcaag gacttcaata tcaagacaga tttaggtcca gcttctcagg
     1141 aagtggatct gattatttca gtggaaggcg tacagcagaa gtaagcgtga aatgttgccg
     1201 gatggcacgc atccggcaat gatatttatt ccgttggatc aggaatgccc agtgttgtat
     1261 tcagacgtcc acgagactta ttaaagatct tattgccatt ttcacgtcct gcccgacggc
     1321 gtcgctgttc ttctggcggt aacgcgcttt cttcgcagca aatctcacta caacaacctt
     1381 tgtatttttc cgcacatact ggacactgaa taaacagcag gtggcagcca tcatttttac
     1441 agttggtatg gctgtcgcac ggcgcaccgc actggtggca atgcgcgata atctcatcag
     1501 atatacgttc gcccatccgc tcgtcaaaaa caaaattttt gccaataaaa cgcaccggca
     1561 agccctgctc gcgcgcctta cgggcgtatt caataattcc gccctcgata tgccagactt
     1621 tgttgaatcc gttatgtttc atccaggcac tggccttttc acaacgaatg ccgccggtgc
     1681 agtacatgac gattttttta tctttatgtg cctgcatcat ctcgactgct tttggcagct
     1741 gctcacggaa ggtatctgcc ggaatttcga gcgcgttttc aaagtgcccc acttcatact
     1801 catagtggtt acgcatgtcg ataaatagtg catcgggatc gtcaagcatg gcgttcactt
     1861 ccgccgcttg cagatactca ccaacattgc tggcatcaaa gtgaggatcg tcaataccgt
     1921 cggcaacaat gcgatcgcgt accttcatgc gcagtaccca gaaggatttc ccgtcatcat
     1981 ccaacgcgat attcaggcgt aaaccctcta aagccgggtc gaaggcatag agctgcgcgc
     2041 gaaatgtttc aacattgctc gcaggtacgc tgatttgcgc gttaatgccc tcatgcgcca
     2101 gatacactcg cccaaaaaca ttcagcgcgg taaacagctg atataaagcg tcacgggtcg
     2161 ccttaggatc ggcgatgtgg aaatacttgt aaaacgaaat ggtggttcgc ggttcgctct
     2221 cagccaacat tttggctttt agcgcgtcgt tggaaatgcg gttgtgtaac actggcatgg
     2281 tgtacggttc ctgcgagatg ggaaagtaaa aatccgcggc atgatatagc aattatcgat
     2341 aattaacatc cacacatttt actctacatt tgcgcattaa aaattatttg ttatttacaa
     2401 gcgcggcaat ttcgcccagt cttcagccac aaattttggt tgcgggcgaa aaaatgcgac
     2461 aatacataca attgcccgaa taggttgaaa aacaggattg atatgacgaa tctacccaag
     2521 ttctccaccg cactgcttca tccgcgttat tggttaacct ggttgggtat tggcgtactt
     2581 tggttagtcg tgcaattgcc ctacccggtt atctaccgcc tcggttgtgg attaggaaaa
     2641 ctggcgttac gttttatgaa acgacgcgca aaaattgtgc atcgcaacct ggaactgtgc
     2701 ttcccggaaa tgagcgaaca agaacgccgt aaaatggtgg tgaagaattt cgaatccgtt
     2761 ggcatgggcc tgatggaaac cggcatggcg tggttctggc cggaccgccg aatcgcccgc
     2821 tggacggaag tgatcggcat ggaacacatt cgtgacgtgc aggcgcaaaa acgcggcatc
     2881 ctgttagttg gcatccattt tctgacactg gaactgggcg cgcggcagtt tggtatgcag
     2941 gaaccgggga ttggcgttta ccgcccgaac gataatccac tgattgactg gctacaaacc
     3001 tggggccgtt tgcgctcaaa taaatcgatg ctcgaccgca aagatttaaa aggcatgatt
     3061 aaagccctga aaaaaggcga agtggtctgg tacgcaccgg atcatgatta cggcccgcgc
     3121 tcaagcgttt tcgtcccgtt atttgccgtt gagcaggctg cgaccacgac cggaacctgg
     3181 atgctggcac ggatgtccgg cgcatgtctg gtgcccttcg ttccacgccg taagccagat
     3241 ggcaaagggt atcaattgat tatgctgccg ccagagtgtt ctccgccact ggatgatgcc
     3301 gaaactaccg ccgcgtggat gaacaaagtg gtcgaaaaat gcatcatgat ggcaccagag
     3361 cagtatatgt ggttacaccg tcgctttaaa acacgcccgg aaggcgttcc ttcacgctat
     3421 taaatctccc ctgccggatg cttcagaatg gcatccggca ttaccacagc aaatccccct
     3481 gatttagcga taaaagctct ctggattgcg ccccctggaa gtcgggcgca taattagtgt
     3541 gcttatcttt tcttcttatg ttcaccgcgc ctggcgcacc aacagcggat tgctatgtca
     3601 ccctgtgaaa atgacacccc tataaactgg aaacgaaacc tgatcgtcgc ctggctaggc
     3661 tgttttctta ccggtgccgc cttcagtctg gtaatgccct tcttacccct ctacgttgag
     3721 cagcttggcg ttaccggtca ctccgccctg aatatgtggt ccggtattgt cttcagcatt
     3781 acatttttat tttcggccat cgcctcaccg ttttggggtg gactcgccga ccgtaaaggc
     3841 cgaaaactca tgctattacg ctctgccctc ggcatgggca tcgtgatggt gttgatggga
     3901 ctggcacaaa atatctggca gtttttgatc ctacgggcgc ttcttgggtt acttggcgga
     3961 tttgtcccca acgctaatgc tcttatcgcc acacaagtac cgcgtaataa aagcggctgg
     4021 gcgctgggta cgctctccac aggcggcgtt agtggtgcgt tgctcggccc aatggctggc
     4081 ggcctgctcg ccgatagcta cggcttacgt ccggtattct ttattaccgc cagtgtgctc
     4141 atactctgct ttttcgtcac cctgttctgc atcagagaaa aattccagcc ggtcagcaaa
     4201 aaagagatgc tgcacatgcg ggaagtggtg acatcactta aaaacccgaa actggtactc
     4261 agcctgtttg tcaccacgtt aatcatccag gtggcgacag gctcaattgc ccccattctg
     4321 acgctgtatg tccgcgaact ggcgggtaac gtcagtaacg tcgcctttat cagtggcatg
     4381 atcgcctcgg tgccaggcgt ggcggctctg ctaagcgcac cacgactcgg caaacttggc
     4441 gatcgaatcg gacccgaaaa gatcctgatt acagcgctga tcttttctgt actgctgttg
     4501 atcccaatgt cttacgttca gacgccattg caacttggga ttttacgttt tttgctcggt
     4561 gccgccgatg gtgcactact ccccgccgta cagacactgt tggtttacaa ctcgagcaac
     4621 cagatcgccg ggcgtatctt cagctataac caatcgtttc gtgatattgg caacgttacc
     4681 ggaccattga tgggagcagc gatttcagcg aactacggtt tcagagcggt atttctcgtc
     4741 accgctggcg tagtgttatt caacgcagtc tattcatgga acagtctacg tcgtcgtcga
     4801 ataccccagg tatcgaactg atttttcgcc tttcatactt gcaaaagcgg agaatcagct
     4861 atccttttcc ctgaaacctc atcaactcaa agggagaatc gtgatgacca tgtacgcaac
     4921 gcttgaagaa gccattgacg ctgcacgcga agaatttctt gcagacaacc ccggcatcga
     4981 cgccgaagat gcgaatgtgc aacagttcaa tgcccaaaaa tacgttttgc aggacggcga
     5041 catcatgtgg caagttgagt tttttgccga cgaaggggaa gaaggtgaat gtttacctat
     5101 gcttagcggt gaagccgcgc aaagtgtttt tgatggcgac tatgatgaga tagagatacg
     5161 ccaggagtgg caggaagaga atacattaca tgaatgggac gagggggaat ttcagcttga
     5221 gccaccgctg gataccgagg aaggacgcgc agcagctgat gagtgggatg aacgttaatc
     5281 actcatacgg gccatgatga atgtcgatcg gcagcagtaa agtatcaaaa accagagaga
     5341 atggcagatc aaggatcgtg acataacgcc aggcggagtc acgcacatcc cattgcacac
     5401 cgggataata ttggttgcca tgcccctgcc ccggtatagt gcgactaata atgctgccac
     5461 agccactcag caaggtcacc atgatgctca ccacaattaa acgcatttga tacctctttc
     5521 gtaaaaaaat accggcataa cgccggtatt tttattggct cgttgttttt gccggatgcg
     5581 gcgtgaacgc cttatccgac ccacaaaatc ttgcaaaatc aataaattgc aggaacgatg
     5641 taggcctgat aagcgtagcg catcaggcaa ctacgttttt attgcgaagc cgcatccggt
     5701 ttacgcaatg ccagtggatt gagctttatc ccttcgtaat aactcaccca tgaagaatat
     5761 ctctccgggg aattccagac gcggaaatgc agacgggcca tcgtcaccgg atcgcttagc
     5821 agcaccaggc gacgatcgcg attcagcttc tctggcgtct cgttcagcgc ctgttcaacg
     5881 tggcggtcac gggcgatttc cagcacctta ctggcgcggt gacgcgcggt cgccattgcg
     5941 gttgccagag cgttaaatga cgggttaaac actgcgtgca taaagccatc atcaagggag
     6001 cgttgacgat tcatctcaag gaaccgatcg gtatcaacca gcacctgcgg cggcgaatac
     6061 tcttccggga tcaggaacag tttccagcgt ttggtgcgca gaccaacggt ggcacggctg
     6121 gaaatcaccg aaacaaacgg tgacaggatc aacgagaaga caatcggtgc cagccagaac
     6181 aggaaacgca gatccaacca cgccatacca acagcccaca ctaaccctaa cagcagctgt
     6241 gagccgtggc gtttgaacgc ttcaccccag gaagtggagt catcatcacg ctgcggtgaa
     6301 ttccacacca cttcccagcc aaggaacgcg ctgacgacga agaccgtatg gaacagcatg
     6361 cgtaccggag ccagcagcac ggaaaacagc acttccagca gcaacgataa tgtaacgcgc
     6421 cagaagccgc cgtattcttt cgttcctttg caccagataa gcaaaatgct caataacttc
     6481 ggcaggaaca acagcaccat ggtcgaagca aaaagtgcaa tcgccagctc aggacgccac
     6541 tgcggccaca ccgggaacaa ctgccgtggt tgcaggaagt attgcggttc ggtcaacgca
     6601 tgtactacct gcaatgcagt agagagcgcg aggaacataa accacagcgg agcggagaga
     6661 taagacatca cgcccgtcag gaacaccgca cggtgaaccg ggtgcatacc cttcaccagg
     6721 aacagacgga agttcatcag gttaccgtgg caccagcggc ggtcacgttt tagctcatca
     6781 agcaagttag gcggcaattc ttcataagaa cccgggagat cgtaagcaat ccagaccccc
     6841 caacctgcac ggcgcatcaa cgccgcttcc acgaagtcat gtgacaggat tgaaccggca
     6901 aaggaacctt cgcccggcag cggagccagt gcgcagtgct cgataaacgg tttcacgcgg
     6961 ataatcgcgt tatgtcccca gtagtgcgac tcgccaagtt gccagaagtg caaaccggct
     7021 gtaaacagtg gcccatacac gcgggtcgcg aactgctgac agcgcgcata cagcgtatcc
     7081 ataccggacg ctttcggcga cgactgaatg atcccggcgt tcgggttggc ttccatcaag
     7141 cgcaccaggc cgcataaaca atcaccggtc attaccgagt cagcatccag caccaccatg
     7201 tagctgtact ggctgcccca gcgacggcag aagtcatcga tattaccgct tttacgcttc
     7261 acgcggcgac ggcggcggcg atagaaaatc tgaccttcgc caccgacttc agcgataagc
     7321 tccatccagg ctttttgctc tgcgacgcag atatccgggt tataactgtc actaagaatg
     7381 tagacatcaa agtgtttggc attcccggtg gcttttactg attcccacgt tgcacgcagg
     7441 ccagcaaaaa cacggttcac gtcttcgtta cagataggca tgatcaacgc cgtgcgatgc
     7501 tccgggttta atggttcatc gccaactgtt gacgcagata tactgtattt atcgcgacca
     7561 ataagcagtt gcaggaagcc cattaacgcc gtccagaatc cggcggacac ccaacagaac
     7621 agtaccgcaa agaggatcag aataccggtt tgcagcatat aaggcagaag ctgcataaag
     7681 gaaacccaca aatcctgacc aaccatatcc ataggattaa tcagcgccca accctgataa
     7741 ggaagaatgg tcttcatata ccaggtcgcg acgacagttt gcgcgagcgt caggatcaac
     7801 agaatgtaac ggcggatggt accgacggta cgccactttt gctcgctctc ctgctcttct
     7861 ttggtcaaac gagccagata gcgcggcgtg acatcgcgtc cacgcaggcg atcccagaaa
     7921 cggcctaccg ggttggtacg ccacgggtcg ggaaacatcg aggagcgttt tgcttctggc
     7981 atcgccttca gctgatcgcg cccttcgtcg tctttaatta actgtccatc agcaagtgaa
     8041 tctggccagg cttgttccag acgcgccttt accgagcctt gcggggaatc atcctcccgc
     8101 gcccaggtgc ggtgttcggc atccagcgcc tgatgaacgg cgcggatatc agtcttcggc
     8161 aatgccgctt tctcgcttgc ggcgatgggc attgcgtcaa tatactcagt tgtcttattc
     8221 attggcaggt aactggtagc tccaggtttc actcaacgtc tgatcggcat tcaccagcgc
     8281 agcacgcatt tcagtggttt tcttggcatc tttcactttc acacgcatca ccagacgcca
     8341 gcctttggta accgggttat agcgcaccgt gctttcaact atctcaccat tatcaccaat
     8401 gctggtttgc gctgtgaccg gggtatcctc tggcagtttt ttcatctctg cgccggtaaa
     8461 atcgaccaca aaggcgatag taccgtcagg ctggcgaatc aggttcgact gcttcacatc
     8521 ccccgttgaa cgacgcgttt gttgcaccca tgcgttatct ggcgcatgca gtttgtcttc
     8581 atcacggctg aaggtgatgg tgtatttaaa gttcatctct ttacccggct ccggcagctg
     8641 atccggcgtc cagtaagcga cgatgttatc gttggtttca tcgttggttg gaatttccac
     8701 cagctcaacg ctgcctttac cccactcccc tttcggagtc acccatgcgc ttggacggag
     8761 atcgtaacga tcatcgagat cttcaaagcg ggagaaatca cgaccgcgct gcaacagacc
     8821 aaagccttgc gggttttcca tggagaagct gctgaccgct aaatgtttcg ggttattcaa
     8881 cggacgccag atccattcgc cattaccggc atggatagag agaccgttag agtcgtgcaa
     8941 ctccggacga tagttatttg caggcgacgg ttggttcggc ccaaacagga acatactggt
     9001 taacggtgca acccccagtt tgccgacttt atcgcgcaga tagattttcg actgcacatc
     9061 cacaaccgtg tcacgccctg gcataaccac gaatttgtaa gcacctgtcg cgcgcggcga
     9121 gtcaagcaat gcatagatgg ttaaacgttt atcagtcggt tttggacgct cgatccagaa
     9181 ctctttgaag cgcggaaatt cttcacccga tggcaaggcg gtatcaattg ccaggccgcg
     9241 ggcagaaagg ccataaacct gacctgcacc aatcacgcgg aaatagctgg ccccgagcat
     9301 gctgacgatt tcatcgtttt tatctttgct gttgatcggg taaagcactt taaaaccggc
     9361 aaagccaagg tcttttaccg tatctttgtc atgctgaaca tcgccgaaag tgaaataatc
     9421 cgggctgtat ttgattcgtt tgactgcggt ggcagtcact tcatttattt tgaccggggt
     9481 atcgaagtac ataccctgat ggtagaactc gagtttgaat ggggtcttca gattgttcca
     9541 gtacgctttg tcatgattaa actggatctg ctgatagtcc gcgtatttca tatcgcggaa
     9601 aacggagggc aagttgcttt tgggcgtctc gtagcctttc ccggctaaag attgagcttg
     9661 ctttgcgaca tcatcaatac tgaaagccca gcttgaagat gtatacaggg ttaacattac
     9721 tgcagcactc aaccaacgca ttttcatcat ttgtagttta tgtttcataa ttagtaagca
     9781 cttccccctt tgtgtgctta tatcgatccg atctatttta atggataacc agccattccg
     9841 acaacagaat cccggtatcg ttcagcacgc tggctcatgg attaagcacc tgattgcaac
     9901 cgttttcact ttccgtgctt ctcctaaaga cagaagcatc gacttcgtgt agggtttgcc
     9961 agcaaattta tttatcaacc gtcttacaga cgcttccatt ctggttagtg gtagcgatga
    10021 tgctatgcat aaaaaccttg aagcctgcgc ctggcgcggt caggtcggtg tcactatggc
    10081 aacaagccta aaggcataac ccgacaggga taagacgaaa aatccgagat tagttaacca
    10141 tatatgaacc cagtacccgc gcaacgtgaa tatttcctcg actccatccg cgcctggctg
    10201 atgttgttag ggataccttt tcatatttct ttaatctatt cgagccatac atggcatgtg
    10261 aatagcgccg aaccatcatt gtggctgacc ctttttaatg acttcatcca ctcgttccgc
    10321 atgcaggtat ttttcgttat atccggctac ttttcctaca tgcttttttt acgctatccc
    10381 ttgaaaaaat ggtggaaagt acgtgtcgaa cgtgtaggta tcccgatgtt aacagccatc
    10441 cccctactga cattaccgca atttattatg ctgcaatatg tcaaaggaaa agcggaaagt
    10501 tggcctgggc tgtcattgta tgacaaatat aatacgttgg cctgggaatt aatatcacac
    10561 ctgtggtttt tactggtgtt agtagtcatg acgacgctgt gcgtatggat atttaagcgc
    10621 atcagaaata atttagaaaa ttctgataaa acgaataaaa aattctcgat ggtaaaacta
    10681 tcggtgattt ttttatgcct cggcatcggt tatgcggtaa taagaagaac gatttttatt
    10741 gtgtatccgc ccattctgag taatggcatg ttcaatttta ttgtcatgca aacgctgttt
    10801 tatttgccgt tctttatcct cggcgcactg gctttcattt tccctcatct taaagccttg
    10861 tttaccacgc cgtctcgtgg ctgtaccctt gcagcagcat tggcgtttgt cgcttattta
    10921 ctcaaccagc gctatggcag tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc
    10981 atgctcctcg gtctgtggat ggtgaatgtg gtcttctcct ttggccaccg tttgcttaac
    11041 ttccagtcag cgcgggtgac ttacttcgtt aacgcatcgc tatttatcta tctggttcac
    11101 cacccgttaa cgctgttttt cggcgcatac attacaccgc acatcacctc caactggctt
    11161 ggttttctct gtggcctgat atttgtagta gggattgcga taattctgta tgaaattcat
    11221 ttgcgcatcc cgttactgaa gtttttgttc tctggtaaac cggttgttaa gcgtgagaac
    11281 gataaagcac cagcccgtta agccacattt acaataacca ttccacgggc aatatcgacg
    11341 ccagtctgac cataacccgc ttccagaaac tggtggcggg ttcttttttg agaataatct
    11401 ctttctttgc atgacgatca acccagttga tccgtcccca cctgtccaga cggagctgcc
    11461 aggccgcatc atactggctc tgaataaagc gtttatcaat taactgtgcc agcgtttcgc
    11521 tctctatcac gaagcccatt tcagtattga gcaatgttga acgcggatcg aaattgaaag
    11581 agccgataaa caccgtttta ccatcgatgc taaaggtttt agcatgcagg ctggctccgg
    11641 aattaccggt tatgccgcga tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt
    11701 catataattc cacgccatag cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga
    11761 cgacagcaac atcgttagcg gcaagagaat tggttaggat cgcaatcttt accccttttc
    11821 tcaccatccg taagagttgc gccacacctg cgcgtgtcgg tacaaaatag gaagagataa
    11881 tatcgatgcg ttcactgggt gagcccatga tatcgaacag gcgctgcggt aacagtgaat
    11941 gccgttttgc cttgccctcc cctttcgccg gatcatcact taataaacgt gtcttcgccc
    12001 agataagcgg caatgttcca tcaaccagat gatttataaa tggactggat tccattttac
    12061 gtaaataacg atgcgtcatg gcatcgttat gccaggaggc gggtaactcg atgcgatccg
    12121 ccatttcacc ctccgggaca tccagcacct gctgtaaggg tgaaaccgat ttgcaatacc
    12181 agtagcgggc gaaatcatcg gcaacgtcct ctaccacggg tcctattgcc atgacatcta
    12241 aatccgaaaa aagtggctcc tcccctgctc caaaataggc atcaccaata tttcgtcctc
    12301 ccaccagggt caccacgcca tcgacagtga aacttttatt gtgcatacgg cgattaagac
    12361 gggaaaagtc ggtgatataa ccaagcggac gcagcaagcg aaacgagaaa ggattaaaaa
    12421 gccggacttc aatgcgtgga tgactgtcaa gcaagcgtaa aatgtcgtca agtccgggcg
    12481 tattgttgtc gtccagcaac aaacggacgc gaacgccacg ctttgctgcg gctaacaggg
    12541 cggaaaacag taaccgaccc gacatatcgt cctgccagat gtaatactga acatcgagcg
    12601 tatgctctgc catttcggcc agacgatacc gggcggcaaa cgcatccaga cttttctcca
    12661 gaggaaaaag gccacactga ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca
    12721 gccggggcaa atcattcatc tccttgttgg gtaaggagtc tttcgtagag gtgggcgttt
    12781 tcttcatcat aacagacaaa gtatacctgt tcgggtaaag cgtgacgggt aataaattct
    12841 gaaacggttt ttactgcgat ttcagccgct gccgcacgag ggtaaccata aaccccagta
    12901 ctgattgcag gaaaagccac tgacgtatag ctgtttgccg ccaccagtcg taggctattg
    12961 agataggcat cctgcaaaag ctggtcttcg ttttgttcac caccacgcca gactggcccg
    13021 acggtgtgca ctacggcttt agcgggaaga tcgcctgcaa gcgtaataac ggcatgcccc
    13081 gtagggcaat cgccctgctg ttgcctgact tttaaacagg catccagtag ggccggaccc
    13141 gctgcgcgat gaatggcccc atcgacgccg ccacctccca ttaatgaagg attagccgca
    13201 ttcacaatca catcaacggc cagtttggta atatcaccct gcacaacatg aatacgcgtt
    13261 ttcataacgc ctccttacac cttcttgttt cttaagtgta cagcaggctt tttggttaaa
    13321 tcgcattttt ctactcagca acgtgagttg ttcagaaact tagttatatt cgatgttcat
    13381 tacagccagc tttttctgtt tatcaatata ctgcactttt actgaggcta cttgcgcgtt
    13441 tttgacattg ccgctcatta acgctttgct ggaataggtt tttcccggaa cagaaccatt
    13501 ctgtgggcag ttcatttcta cgggtgatga ctgggtgctg acgtcacatg ccggttccac
    13561 aatttggcca taaaaatgga tcacgccccc ttgcgcggtg cttcctgcga tggcaataaa
    13621 aggaaaaaac aaactgccga gcataagaga gtttaaaaaa ggtctgaaca tttattctat
    13681 ttcctcaatg attagtcatc cttgagggtt gtgttatcca tactttcagg ttggctgatt
    13741 cgcagcagac cattctctcc agattcatct tatgctcgat atttcaacaa attaagattt
    13801 ttctgaagag ggcggccatt gttgtgataa atgaagtgac tgtccatcag aaacagtaac
    13861 aactattttc acccgatcgt ccggggaaat atttaaactc aacttcgtca aagcaatggg
    13921 ttgattagca ggcaatgaaa gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc
    13981 ttcgcgcagg gacaatattt gtactctgca cagacaagat tgagtaagag tgacttcagg
    14041 aataatggta tacacatccc cttgctgggt cgtattaaag gttatctggc tggaaagtgc
    14101 cgcaaggagt aataacgcat tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg
    14161 tttctgtaat acaaatgatg tattagtact gatgagcggt cgcgttgtta ccaaagccaa
    14221 cctgagtcac gttgacggag gagttagatg cagtctggtc aactgcagca ccgttgccac
    14281 caccgaactg tttaaccgtc atttcagaat ttttgccgtt ccactgatca agagtagcgc
    14341 tgttaccgaa gccacgttgg gtcagatcga ttgagctgtc atccgagccc tgaccaacat
    14401 ctgcaccatt accgccgcca tgctgggtaa tagtcaagtc agagttacgg gcatcagttt
    14461 gcagagcaag tgcagagtta ccgccaccgt actggtaaat gttcagctct gaatttgggc
    14521 cgctattatt accgccacca ccgtggttac cgccgccgcc gtactgagga acaacacctg
    14581 ccagagcgct acaggagaat acgattgctg caattgctgc tacttttaaa agtttcatgt
    14641 aaaaccccca tcggattgat ttaaaagtcg aatggaaatt aacgttgtgt cacgcgaata
    14701 gccatttgcg actgtctctg cactacaatt gccgtttttt gagtaccata ctgtgtaata
    14761 tttgctttat taccagaacc tttctggata atcatcgcag tattaccata agcaccttgc
    14821 gaaatactgg catcgttggc actgcccgcc tgatcaatat atgcaaggtt ataatctcct
    14881 gtctggtcaa tctttgcccg attgctacta ccttcttgcg caacaaccgc caaaagtttt
    14941 gagcctccct gccgtaactg agcactatta ttcgtcccag cttgaccaat tatggctgcc
    15001 tgattaaatg aagacttact caattcattt accgcgaagt tatattctga attagctaaa
    15061 tcataacctg ctgcggctgc aatcccaggc gcacccagta ttgttaacat cataaataac
    15121 aatttgtttt tcatgttgtc accctggacc tggtcgtaca tttaagaaat taaatcattt
    15181 caacttggtt gttaacgcaa cctgtatttt gttaacgctg cgttacgatg gaaagtatgt
//
LOCUS       DAZKIV010000111        15172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZKIV010000111 DAZKIV010000000
VERSION     DAZKIV010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.111"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..444
                     /locus_tag="ACHOT6_003351"
     CDS             109..444
                     /locus_tag="ACHOT6_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFP9948515.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            602..1045
                     /gene="yjdN"
                     /locus_tag="ACHOT6_003352"
     CDS             602..1045
                     /gene="yjdN"
                     /locus_tag="ACHOT6_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFP9948516.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1179..1967
                     /gene="phnC"
                     /locus_tag="ACHOT6_003353"
     CDS             1179..1967
                     /gene="phnC"
                     /locus_tag="ACHOT6_003353"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFP9948517.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            1992..3008
                     /gene="phnD"
                     /locus_tag="ACHOT6_003354"
     CDS             1992..3008
                     /gene="phnD"
                     /locus_tag="ACHOT6_003354"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9948518.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3114..3893
                     /gene="phnE"
                     /locus_tag="ACHOT6_003355"
     CDS             3114..3893
                     /gene="phnE"
                     /locus_tag="ACHOT6_003355"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFP9948519.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            3914..4639
                     /gene="phnF"
                     /locus_tag="ACHOT6_003356"
     CDS             3914..4639
                     /gene="phnF"
                     /locus_tag="ACHOT6_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFP9948520.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4640..5092
                     /gene="phnG"
                     /locus_tag="ACHOT6_003357"
     CDS             4640..5092
                     /gene="phnG"
                     /locus_tag="ACHOT6_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFP9948521.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5089..5673
                     /gene="phnH"
                     /locus_tag="ACHOT6_003358"
     CDS             5089..5673
                     /gene="phnH"
                     /locus_tag="ACHOT6_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFP9948522.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGITEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAVPRTTHVEVC"
     gene            5673..6737
                     /gene="phnI"
                     /locus_tag="ACHOT6_003359"
     CDS             5673..6737
                     /gene="phnI"
                     /locus_tag="ACHOT6_003359"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFP9948523.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEKNHG"
     gene            6730..7575
                     /gene="phnJ"
                     /locus_tag="ACHOT6_003360"
     CDS             6730..7575
                     /gene="phnJ"
                     /locus_tag="ACHOT6_003360"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFP9948524.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7572..8330
                     /gene="phnK"
                     /locus_tag="ACHOT6_003361"
     CDS             7572..8330
                     /gene="phnK"
                     /locus_tag="ACHOT6_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFP9948525.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8507..9187
                     /gene="phnL"
                     /locus_tag="ACHOT6_003362"
     CDS             8507..9187
                     /gene="phnL"
                     /locus_tag="ACHOT6_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFP9948526.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTIGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9184..10320
                     /gene="phnM"
                     /locus_tag="ACHOT6_003363"
     CDS             9184..10320
                     /gene="phnM"
                     /locus_tag="ACHOT6_003363"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFP9948527.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10320..10877
                     /gene="phnN"
                     /locus_tag="ACHOT6_003364"
     CDS             10320..10877
                     /gene="phnN"
                     /locus_tag="ACHOT6_003364"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFP9948528.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10864..11298
                     /gene="phnO"
                     /locus_tag="ACHOT6_003365"
     CDS             10864..11298
                     /gene="phnO"
                     /locus_tag="ACHOT6_003365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFP9948529.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11300..12058
                     /gene="phnP"
                     /locus_tag="ACHOT6_003366"
     CDS             11300..12058
                     /gene="phnP"
                     /locus_tag="ACHOT6_003366"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFP9948530.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHCDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12205..12534)
                     /gene="yjdP"
                     /locus_tag="ACHOT6_003367"
     CDS             complement(12205..12534)
                     /gene="yjdP"
                     /locus_tag="ACHOT6_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFP9948531.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12603..12920)
                     /locus_tag="ACHOT6_003368"
     CDS             complement(12603..12920)
                     /locus_tag="ACHOT6_003368"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP9948532.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13173..15158
                     /gene="yjcS"
                     /locus_tag="ACHOT6_003369"
     CDS             13173..15158
                     /gene="yjcS"
                     /locus_tag="ACHOT6_003369"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFP9948533.1"
                     /translation="MNNSRLFRLRRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQPQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT         3432 a         4519 c         4178 g         3043 t
ORIGIN      
        1 tcagcagtac ccccgacaaa ctcagggatt ttgtgtataa ttgcggcctt tttcggcaat
       61 ctgccgtttt ttggcgcttt tgccctgctg acttttgagg aaatccacat gtcattacca
      121 cactgcccaa aatgcaactc cgaatacact tacgaagata acggcatgta catctgcccg
      181 gaatgtgcct acgaatggaa cgacgcagaa cctgcacagg aaagcgacga gctgatcgtt
      241 aaagatgcta acggcaatct gctggctgac ggcgacagcg ttaccatcat taaagatctg
      301 aaggtgaaag gtagctcttc gatgctgaaa attggcacca aagtgaaaaa catccgcctg
      361 gttgaaggcg accataacat cgattgcaaa atcgacggtt ttggtccgat gaaactgaaa
      421 tctgagtttg tgaaaaagaa ctgattgtat tgtgatcggt aagccggata aggcgctcgc
      481 gccgcatccg gcaacgatgc cgactgcctg atgcgacgct tgcgcgtctt atcaggcagc
      541 ctctcatctg tataaatttc gaactacact taactggctt ctcttaactg aggtcaccat
      601 catgccgtta agtccctacc tctcttttgc cggtaactgt gccgacgcaa ttgcttatta
      661 tcaacgtacg ttgggcgctg aactgctcta taaaatcagc ttcggcgaaa tgccaaaatc
      721 agcgcaggac agcgccgaga actgcccttc cggaatgcaa tttcccgata ccgccatcgc
      781 ccacgccaat gtgcgcattg ccggaagcga catcatgatg agcgatgcca tgccgtcagg
      841 aaaagccagc tactccggct ttacgctggt gctcgattcg caacaggtcg aagaaggaaa
      901 acgctggttt gacaatcttg ccgctaacgg aaaaatcgaa atggcctggc aggaaacttt
      961 ctgggcgcat ggctttggca aagtcaccga taaatttggc gtaccgtgga tgattaatgt
     1021 cgtcaaacaa caaccaacgc aataacccgc cgggaggccc gccctcccgc actgtcatcg
     1081 aattcccgtt aactcttcat ctgttagtca cttttaatta accaaatcgt cacaataatc
     1141 cgccacgatg gagccacttt tttagggagg catgcatcat gcaaacgatt atccgtgtcg
     1201 agaagctcgc caaaaccttc aatcagcatc aggcgctgca tgcggttgat ctgaacattc
     1261 atcacggtga aatggtggct ctgcttgggc cgtcgggttc cggcaaatcc acccttttac
     1321 gtcacttaag cggtttgatt accggcgata aatccgccgg cagccatatc gagctgctgg
     1381 gccgcacagt ccagcgcgaa ggccgtctgg cgcgcgatat ccgcaaaagc cgcgccaaca
     1441 ccggctacat cttccaacaa ttcaacctgg tgaaccgcct gagcgtactg gagaacgtgc
     1501 tgattggcgc gctcggcagc acgccgttct ggcgcacctg ttttagctgg tttacccgcg
     1561 agcagaaaca acgcgcgtta caggcgctga cccgcgttgg catggtgcat tttgcccatc
     1621 aacgcgtttc caccctctcc ggcggacagc agcagcgtgt ggcgattgcc cgcgcgctga
     1681 tgcagcaggc gaaggtgatt ctggccgatg aacccatcgc ctcgctggac ccggaatccg
     1741 cccgcatcgt gatggacacc ctgcgcgaca tcaaccagaa cgacggcatc accgtggtcg
     1801 tcacgctgca tcaggtggat tacgccctgc gctactgcga acgcatcgtc gccctgcgcc
     1861 aggggcacgt tttctacgac ggcagcagcc aacagtttga taacgaacgt tttgaccatc
     1921 tctaccgcag cattaatcgc atcgaagaga acgcgaaagc tgcctgacat ccccatcatt
     1981 gaggaaaacg aatgaacgct aagataattg cctcgctggc cttcaccagc atgttcagcc
     2041 tcagcaccct gttaagcccg gcacacgccg aagagcagga aaaggcgctg aatttcggca
     2101 ttatttcaac ggaatcacag caaaacctga aaccgcaatg gacgccattc ttacaggata
     2161 tggagaagaa gctgggcgtg aaggtgaacg ccttctttgc cccagactac gcaggcatta
     2221 tccagggaat gcgcttcaat aaagtggata tcgcctggta cggcaacctg tcggcgatgg
     2281 aagcggtgga tcgcgccaac ggtcaggtct tcgctcagac cgtcgcggcg gacggatcgc
     2341 cgggttactg gagcgtgttg atcgtcaaca aagacagtcc gatcaacaac ctgaacgatc
     2401 tgctggcgaa gcgtaaagat ctcaccttcg gcaatggcga tcctaactcc acctctggct
     2461 tcctcgtccc cggctactac gtcttcgcca aaaacaatat ctccgccagc gacttcaagc
     2521 gcaccgtcaa cgccgggcat gaaaccaacg cgctggccgt cgccaacaag caggtggatg
     2581 ttgccactaa caacaccgaa aacctcgaca agctgaaaac ctccgcgccg gagaagttga
     2641 aagaactgaa agtgatctgg aagtcgccgc tgatcccagg cgatccgatc gtctggcgta
     2701 aaaacctttc cgaaaccacc aaagacaaga tctacgactt ctttatgaac tacggcaaaa
     2761 cgccggaaga aaaagcggtg ctggaacgcc tgggctgggc gccgttccgc gcttccagcg
     2821 acctgcaact ggtgccgatt cgccagctcg cgctgtttaa agagatgcag ggcgtgaaaa
     2881 gcaataaagg actgaatgag caggacaagc tggcaaaaac caccgagatt caggcgcagc
     2941 tggatgacct ggaccgcctg aacaacgcgt taagcgcgat gagttcggtg agtaaagcgg
     3001 tgcagtaaat cgtaggtcgg ataagacgcc ccggcgtcgc atccgacaat gtgcaggcgt
     3061 tgatgccgga tgcggtgcaa gcaccttatc cggcctacag accggagcca aacatgcaaa
     3121 ccatcaccat cgccccaccc aagcgcagct ggttctcgct tctgagctgg gccgttgttc
     3181 tcgccgtgtt ggtcgtctcg tggcagggcg cggaaatggc cccgctcacg ctgattaaag
     3241 acggcggcaa catggcgacg ttcgccgccg acttcttccc gcccgatttc agccagtggc
     3301 aggattacct caccgaaatg gccgtcacgc tgcaaatcgc cgtctggggc acggcgctgg
     3361 cggtggttct ctccatcccc ttcggcctga tgagcgccga aaacctggtg ccgtggtggg
     3421 tttaccagcc cgttcgccgc ctgatggacg cctgccgcgc cattaacgaa atggtcttcg
     3481 ccatgctgtt cgtggtcgcc gtcggcctcg gcccgttcgc tggcgtgctg gcgctgttta
     3541 tccacaccac cggcgtgctc tccaaactgc tttccgaagc ggtggaagcg attgaacccg
     3601 gcccggtgga aggcattcgc gccaccggcg ccaacaagct cgaagagatc ctctacggcg
     3661 tgctgccgca ggtgatgccg ctgctgatct cctactccct ctatcgcttc gaatccaacg
     3721 tccgctcggc gaccgtcgtc ggcatggtcg gtgcaggcgg gatcggcgtc accctgtggg
     3781 aagcgattcg cggtttccag ttccaacaaa cctgcgccct gatggtgctt atcatcgtca
     3841 cggtcagcct gctggatttc ctctctcaac ggttgcgtaa gcactttatc tgataagcga
     3901 ggcattgata tctatgcact tgtctacaca tccgaccagc tacccaacac gctatcagga
     3961 gatagccgca aaacttgagc aggagcttcg tcaacactat cgctgcggcg actatcttcc
     4021 cgccgagcag cagctggcgg cgcgctttga ggtaaatcgc cacaccctgc gccgcgccat
     4081 cgaccaactg gtggaaaaag gctgggtaca gcgccgtcag ggcgtcggcg tgctggtgct
     4141 gatgcgcccg ttcgattacc cgctcaacgc ccaggcgcgt tttagccaga acctgctgga
     4201 tcagggcagc catcccacca gcgaaaaact gctgtcggtg ctgcgcccgg catctggtca
     4261 cgtcgctgac gcgctgggga taaccgaggg ggagaacgtc atccacctgc gcaccctgcg
     4321 ccgggtcaac ggcatcgcgc tctgtttaat cgaccactac tttgcggacc tcaccctctg
     4381 gccgacactg caacgtttcg acagcggttc gctgcacgat tttctgcgcg agcaaaccgg
     4441 aattgcgctg cgccgcagcc agacgcggat cagcgcccgc cgcgcccagg ccaaagagtg
     4501 ccagcgcctt gaaatcccga atatgtcgcc gctgctgtgc gtgcgcaccc ttaaccaccg
     4561 tgacggtgaa agcagcccgg cggagtactc cgtcagcctg acgcgcgccg acatgattga
     4621 attcactatg gagcactgaa tgcacgcaga taccgcgacc cgccagcact ggatgtccgt
     4681 actggcgcac agccaaccgg ctgaactggc agcccgcctg aacgcgctga acatcaccgc
     4741 cgactatgaa gtgatccgca ctgccgaaac tggcctggtg cagattcagg cgcgaatggg
     4801 cggtaccggc gaacgctttt ttgccggcga cgccacgctg acccgcgccg ccgtacgcct
     4861 gactgacggc accctcggct acagctgggt gctggggcgt gataaacagc acgccgaacg
     4921 ctgcgcgctg attgatgcgc tgatgcagca atctcgccac tttcaaaact tatcagaaac
     4981 ccttattgcc ccgctggacg ctgaccgtat ggcacgcatt gccgcacgcc aggccgaagt
     5041 gaacgccagc cgggtcgact tctttacgat ggttcgcgga gacaacgcat gaccctggaa
     5101 accgctttta tgcttcccgt gcaggatgcc cagcacagtt ttcgtcgcct gttaaaggcc
     5161 atgagcgagc cgggcgtgat tgtcgccctg catcagctca aacgcggctg gcagccgctg
     5221 aatatcgcca ccaccagcgt gctgctgacg ctggccgata acgacacgcc ggtgtggctt
     5281 gccacgccat taagtaacga tatcgtcaat cagagcctgc gttttcatac caacgcgccg
     5341 ctggtcagcc agccggaaca ggcgaccttc gcggtgacgg atgaggcgat ttccagcgaa
     5401 cagctcaacg ccctttccac cggcaccgcc gttgcgccgg aagcgggcgc gacgctgatt
     5461 ttacaggtcg ccagcctgag cggcgggcgc atgctgcgcc tcaccggcgc gggcattacc
     5521 gaagaacgaa tgatcgctcc gcagctgccg gagtgcattc tgcacgaact caccgagcgc
     5581 ccgcacccgt tcccgctcgg catcgacctg atcctgacct gcggcgagcg cctgctggct
     5641 gttccgcgaa ccactcatgt ggaggtgtgc tgatgtacgt tgccgtgaaa gggggcgaaa
     5701 aggcgatcga cgccgcccac gccctgcaag agagccgacg ccggggcgat accgatttgc
     5761 ccgaactgag cgtcgcccag attgaacagc agcttaacct cgcggtggat cgcgtgatga
     5821 cagaaggcgg cattgccgac cgcgaactgg cggcgctggc gctgaaacag gccagcggcg
     5881 ataacgttga agcaattttc ctgctgcgcg cctaccgcac cacgctggcg aaactggcgg
     5941 taagcgagcc gctcgacacc acagagatgc gtctcgaacg gcgtatttcg gcggtttata
     6001 aagacattcc cggcggccag ctgcttggcc caacctacga ctacacccat cgcctgctcg
     6061 atttcaccct gctggcaaac ggtgaagcgc cgacgctgac caccgccgac agcgaacaac
     6121 agccgtcgcc gcacgttttc agcctgctgg cgcgtcaggg gctggcgaag tttgaagaag
     6181 atagcggcgc acagccggat gacatcaccc gcacgccgcc ggtttacccc tgctcacgct
     6241 cctcccgcct gcaacaatta atgcgcgggg acgaaggcta tttgctggcg ctggcctact
     6301 ccacccagcg tggttacggg cgcaatcacc cgttcgcggg cgagatccgc agtggttaca
     6361 tcgacgtgtc gattgtgccg gaagagctgg gatttgcggt aaacgtcggc gaactactga
     6421 tgaccgagtg tgaaatggtc aacggtttta tcgacccgcc ggatgagccg ccgcacttca
     6481 cgcgcggcta cgggctggta ttcggcatga gcgagcgcaa agcgatggca atggcgctgg
     6541 tcgatcgtgc gttgcaggct ccggaatacg gcgagcacgc gacaggcccg gcgcaggatg
     6601 aagagtttgt gctggcacat gccgacaacg tcgaagccgc aggctttgtc tcgcacctca
     6661 aactccccca ctacgtcgat ttccaggccg aactggagct actcaaacgt ctgcaacagg
     6721 agaagaacca tggctaatct gagcggctac aactttgcct acctcgacga gcagaccaaa
     6781 cgcatgatcc gccgcgctat cttaaaagcg gtggcgatcc ccggttatca ggtgccgttt
     6841 ggcgggcgcg agatgccgat gccatacggc tggggaaccg gcggcataca gctcaccgcc
     6901 agcgtgattg gcgaaagcga cgtgctgaag gtgattgacc agggtgcgga tgacaccacc
     6961 aacgccgtgt cgattcgcaa cttctttaag cgcgtgaccg gggtaaacac cactgaacgt
     7021 acggacgatg cgacgcttat ccagacgcgt caccgcatcc ccgaaacgcc gctgaccgaa
     7081 gatcagatca ttatcttcca ggtgccaatc ccggaaccgc tgcgctttat cgagccgcgc
     7141 gaaacggaaa cccgcaccat gcacgcgctg gaagagtacg gcgtgatgca ggtgaaactg
     7201 tatgaagata tcgcccgctt cggtcatatc gccactacct acgcctatcc ggtgaaggtg
     7261 aacgggcgct acgtaatgga cccgtcgccg atcccgaaat tcgataaccc aaaaatggac
     7321 atgatgcccg ccctgcaact gttcggcgcg gggcgcgaga agcgcatcta tgcggtgccg
     7381 ccgtttaccc gcgtggaaag tctcgatttc gacgatcacc cgttcaccgt tcagcagtgg
     7441 gatgagccat gcgccatctg cggatcgacc cacagctatc ttgatgaagt ggtgctggat
     7501 gacgccggaa accgcatgtt tgtctgctcc gataccgatt attgccgcca acagagcgag
     7561 gcaaaaaacc aatgaatcaa ccgttacttt cggtcaataa cctgacccac ctttacgcgc
     7621 cgggcaaagg ctttagcgat gtctcttttg atttatggcc gggggaagtg ctgggcattg
     7681 tcggggagtc cggctccggg aagaccacgc tgctgaaatc aatctccgcg cgcctgacgc
     7741 cgcagcaggg ggagattcac tatgagagcc gttcgctgta cgcaatgagc gaggccgacc
     7801 gccgtcgcct gctgcgcacc gaatggggcg tagtgcatca gcatccgctc gacggcctgc
     7861 gccgccaggt gtcggcaggc ggcaatatcg gcgagcggct gatggcgacc ggggcacgtc
     7921 attacggcga tattcgtgcc accgcgcaga agtggctgga agaggtggaa attcccgcca
     7981 accggatcga cgacctgccg accacctttt ccggcggcat gcagcaacgt ttgcagatcg
     8041 cccgcaacct ggtgacgcat ccgaagctgg tgtttatgga cgagccgacc ggcggactgg
     8101 atgtgtcggt acaggcacgc ctgctcgacc tgctgcgcgg cctggtggtg gagctgaacc
     8161 tcgcggtagt gattgtcacc cacgatttag gcgtcgcccg cctgctggcg gaccgtttgc
     8221 tggtgatgaa gcaggggcaa gtggtggaga gtgggttaac cgaccgcgtg ctcgacgacc
     8281 cgcaccatcc gtatacacaa ctgctggtgt catcggtttt gcagaattga atgcaacacc
     8341 gtgcgcgctg atcccctcac cccggccctc tcccggaggg agagggagaa aaccggcgca
     8401 atattcaaca ccgcgcacaa tcggtcccct cgccccttcg gggagagggt tagggtgagg
     8461 ggcaatgcgc gcaccgctcc ccaatatgcg aaccacgagg cgaaaaatga ttaacgtaca
     8521 aaacgtcagt aaaaccttca tcctgcacca gcaaaacggc gtgcgcctgc ccgtcttaca
     8581 acgcgcctcg ctcaccgtca acgcaggtga atgcgtggtg ctccacggcc attccggcag
     8641 cggcaaatca actctgctac gctcgctgta cgccaactat ctgcccgacg aagggcaaat
     8701 ccagatcaaa cacggtgacg aatgggtaga cctggtcacc gcgccagcgc gcaaagtggt
     8761 ggagatccgc aaaactacca tcggctgggt gagccagttt ctgcgcgtca tcccgcgtat
     8821 ctcagcactg gaagtggtga tgcagccgct gctcgataca ggcgttccgc gtgaagcctg
     8881 cgccgctaaa gccgcgcgtc ttctcacccg cctgaacgtg ccggaacgcc tgtggcacct
     8941 ggcaccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc gcggctttat
     9001 cgtcgactac cccattctgc tgctcgacga acctaccgcc tcgctggacg ccaaaaacag
     9061 cgccgcggtg gtggaactga ttcacgaagc caaagcccgt ggcgcagcca tcgtcggcat
     9121 cttccatgac gaagctgtac gtaatgacgt cgccgaccgc ctgcacccaa tgggagcctc
     9181 ttcatgatta tcaataacgt taagctggtg ctggaaaacg aggtggtgca cggttcgctg
     9241 gaggtgcagg atggcgaaat ccgcgccttt gccgaaagcc agagccgcct gccagaagcg
     9301 atggacggcg aaggtggctg gctactaccc ggcctgattg agctgcatac cgacaacctc
     9361 gataaattct tcaccccgcg cccgaaagtc gactggcccg cccattcggc gatgagcagc
     9421 cacgacgcgc tgatggtggc aagcggcatc accaccgtac tggacgccgt ggcgattggt
     9481 gatgtgcgcg acggcggcga tcggctggag aatctggaga agatgatcaa cgccatcgaa
     9541 gagacgcaaa aacgcggcgt caaccgcgcc gagcaccgcc tgcacctgcg ctgcgaactg
     9601 ccgcatcaca ccacactgcc gctgtttgaa aaactggttc agcgcgaacc ggtgacgctg
     9661 gtgtcgctga tggaccactc accgggccag cgccagttcg ctaaccgcga gaagtatcgc
     9721 gaatattatc agggcaaata ctccctcacc gatgcgcaga tgcagcagta cgaagaagag
     9781 caattggcgc tcgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc cgccctgtgc
     9841 cgcgcgcgac aaattgcgct cgccagccac gatgacgcca cgcacgccca cgttgccgaa
     9901 tcccaccaac ttggcagcgt gatcgccgaa tttcccacca cgttcgaagc ggcggaagcc
     9961 tcgcgtaagc atggcatgaa cgtgctgatg ggtgcgccaa atatcgtgcg tggcggctcg
    10021 cactccggca atgtggcggc cagtgaactg gcgcagcttg gtctgctgga tatcctctct
    10081 tccgactact accccgccag cctgctggat gcggcgttcc gcgtcgccga tgacgagagc
    10141 aaccgcttta cgctaccgca ggcggtgagg ctggtgacca aaaatccggc gcaggcgctg
    10201 aatcttcagg atcgcggggt gattggcgag ggtaaacgcg ctgacctggt gctggcgcat
    10261 cgccaaggca atcacattca tatcgaccac gtctggcgtc agggtaaaag ggtgttctga
    10321 tgatgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagac agcctgctgg
    10381 cggaactccg cctgcgggaa caaacgcagt tactggtggc gcatcgctac atcacgcgcg
    10441 acgccagcgc cggaagtgaa aaccatatcg ccctgagtga gcgggagttt tttacccgcg
    10501 cggggcaaaa cctgctggcc ttaagctggc acgctaacgg tctgtattat ggcgtcggca
    10561 tcgagattga cctctggctg cacgctggat tcgacgtagt cgtcaacggt tcacgcgcgc
    10621 atttgccgca ggcacgggcg cgctatcaat cggcgctgct gcccgtctgt ttacaggttt
    10681 cgccggagat cctgcgccag cgcctggaaa accgtggccg tgaaaacgcc agtgaaatta
    10741 acgcccgcct ggcgcgcgcc gcccgctata ctccacagga ttgccatacg ctcaacaatg
    10801 acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gatccatcag aaggagaaac
    10861 accatgcctg cttgtgagct tcgcccggcc acgcagtacg ataccgacgc ggtttacgcg
    10921 ctgatttgtg agctaaaaca ggcggagttt gaccaccatg cgtttcgcgt gggctttaac
    10981 gccaacctgc gcgacccaaa catgcgctac catctggcgc tgctggatgg cgaagttgtc
    11041 ggcatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaactggat cggcgaaatt
    11101 caggaactgg tggtgatgcc gcaggcgcgc ggtctgaacg tcggcagtaa attactggca
    11161 tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgagctttc caccaacgtg
    11221 aagcgccacg acgcacaccg tttttacctg cgtgaaggct acgagcagag ccacttccgc
    11281 ttcaccaagg cgctgtaaca tgagcctgac cctcacgctc accggcactg gcggcgcaca
    11341 gggcgttccg gcatggggct gcgagtgcgc ggcctgcgcc agagcgcggc gctcgccgca
    11401 gtatcgccgc cagccatgca gcggcgtagt gaagtttaac gacgcgatca ccctgatcga
    11461 cgccgggcgg cacgatctca ccgatcgctg gtcgcctgga tcgttccagc agtttttgct
    11521 gacgcactac catatggatc acgtccaagg gctatttccg ctacgctggg gcgtgggcga
    11581 tgtgatcccg gtctacggcc cgccggatga acagggctgc gacgatctgt ttaaacatcc
    11641 gggcctgctt gatttcagcc acacagtgga accgtttgtg gtgtttgatt tgcaggggtt
    11701 gcaggtcacg ccgctgccgc ttaaccactc aaaactgacc ttcggttatc tgctggaaac
    11761 ggcacatagc cgggtggcgt ggctgtctga caccgctggc ctgccggaaa aaacgctgaa
    11821 atttttactc aataatcatc cgcaggtgat ggtgattgat tgcagtcacc cgccgcgcgc
    11881 ggatgcaccg cgtaatcact gtgatttaaa taccgtgctt gcgctgaatc aggttatccg
    11941 ctcgccacag gtgattctga cccatatcag ccaccagttt gatgcgtggc tgatggaaaa
    12001 cgcactaccg tcagggtttg aggtggggtt tgatgggatg gagattgggg tggcgtgatg
    12061 agagggaatg tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg gcgaggggac
    12121 cgattgtgct caatattgaa tattgcgctc gttttctccc tctccccatt ggggtgaggg
    12181 gcgatgcctg ctccataccc aacctcatcg cccatactca tcttccattc tccgctcttc
    12241 atcctccagt tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca gctgcctgcg
    12301 gcgatcttca aactggcggc ggcggtcgtc atattgtctg cgccgatcgt cgctcacttc
    12361 acgctgccag ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag ggttataagc
    12421 gtcattaatc gcctgctgaa tattaccaat agtatcgtcg ataatatcag cctgggccgg
    12481 aacggtggac agcgtgagca gggtgaataa aagaaatagc ggaaagcgtt tcattagcca
    12541 acctcaaaaa gaaactctat ccacattagt cattactcat ccatgcaagt agtggatgaa
    12601 tctcaatttc tccgctgctc tattgccgta atcgcctcca cgcgttgttg atgacgaccg
    12661 ccttcgtact gtgcgcccag ccacgcatcc acaatcattt ttgccagttc gaggccaacc
    12721 actcgtgaac caaaagccag tgcaacttca ctggcataat gcggataatc agtgcgctct
    12781 gacgacctgg tgtctttatc aatcacctca ctcattgacc atgaataaaa ggctggcaca
    12841 gcaacgcaga atcctgcggt gccagcgaca tgaccgccca ttgctccggt acgtttttta
    12901 aaaagaaaca ccggttgcat tgataaattg tgctcagtat ttgccgaagt acaatatata
    12961 taataaatat ataaaacacc tcatattatt tacagagata ataaattcgc gtaattttcc
    13021 cccttcagca caaatgccta ataaaatgga tcgttccgct cacacttcca cttattaaag
    13081 tcgcaaattc ccaattatga taaccccagc tttaaatgac gtattttgca tggattacat
    13141 atttacgaca taaatgataa atggagattt atatgaataa ctctcggtta ttccgtttgc
    13201 gcagaattgt tattgcgtta actgccgcca gcggcatgat ggtaaatacc gcttacgcaa
    13261 cagatgaagc gaaagccgcc actcaatata cccaacaggt taatcagaat tacgccaaat
    13321 cattaccgtt tagcgatcgt caggattttg acgacgctca gcgtggattt atcgctccgc
    13381 tgctggatga aggtattctg cgcgatgcta atggcaaacc atattatcgc ggggaagatt
    13441 ataaatttga tatcaatgcg cccgcgcccg aaaccgttaa ccctagcctg tggcgtcagt
    13501 cgcagttaaa cggtatttcc ggcctgttta aagtcaccga cagaatgtac caggttcgcg
    13561 gtcaggatat ctccaacatc acctttatcg aaggtgatac cgggattatt gtcatcgacc
    13621 cgctggtgac gccaccgagc gcaaaagccg cccttgacct ttacttccaa aatcgcccgc
    13681 aaaaaccgat tgttgcggtt atttataccc acagccatac cgaccactat ggcggcgtga
    13741 aaggcattat ctctgaagcc gatgtgaaat ccggtaaagt gcaggttatc gcccctgctg
    13801 gctttatgga cgaagccatc agcgaaaacg tactggcggg taatattatg agccgccgtg
    13861 cactttactc ctacggcctg ctgctggcgc ataaccctca gggtaacatc ggcaatggtc
    13921 ttggcgtaac gctggcatcg ggctacccga gcatcatcgc accgaacaaa accatcacca
    13981 aaaccggtga gaagatgatt atcgacggcc tggagtttga cttcctgatg acccctggta
    14041 gcgaagcacc agccgaaatg cacttctata ttccggccct gaaagcgctg tgtaccgccg
    14101 agaacgccac gcataccctg cacaacttct acactctgcg cggcgcgaaa acccgcgaca
    14161 ccagcaagtg gaccgagtat ctgaacgaaa cgctggatat gtggggtaac gacgcggaag
    14221 tcctgtttat gccgcacacc tggccggtct ggggcaataa gcatatcaat gattatattg
    14281 gtaaatatcg cgatactatc aagtacattc acgaccagac cctgcacctg gcgaaccagg
    14341 gctacaccat gaatgaaatc ggcgacatga ttaaactgcc gcctgcactt gccaataact
    14401 gggccagccg tggctattac ggttctgtca gccacaacgc ccgcgcggtg tataacttct
    14461 atcttggcta ttacgacggt aacccggcta acctgcatcc gtatggtcag gtggagatgg
    14521 gtaaacgtta cgtgcaggcg ctgggcggtt ctgcccgtgt catcaacctg gcgcaagaag
    14581 cgaacaagca aggtgattac cgctggtcgg cagaactgct gaaacaggtg attgccgcca
    14641 acccgggtga ccaggtcgcg aagaatctgc aagcgaataa ctttgaacag ctgggctatc
    14701 aggccgagtc cgccacctgg cgcggtttct acctgaccgg cgcgaaagag ctgcgcgaag
    14761 gggtgcataa gttcagccac ggcaccaccg gttccccgga caccattcgc gggatgtcgg
    14821 tcgaaatgct gttcgacttt atgtccgttc gcctcgatag cgcgaaagcc gcgggtaaaa
    14881 atatcagcct gaacttcaat atgagcaatg gcgataacct caacctgacg ctgaacgata
    14941 gcgtgcttaa ctaccgtaaa acgctgcaac cgcaagctga cgcctctttc tacatcagcc
    15001 gtgaagatct gcacgccgtg ctgaccgggc aagccaaaat ggcggatctg gtaaaagcga
    15061 agaaagccaa aattattggc aatggcgcga aactggaaga aattatcgcc tgtctggata
    15121 atttcgattt gtgggtgaat atcgtaaccc caaattaatg tcactcgctt tt
//
LOCUS       DAZKIV010000112        15075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZKIV010000112 DAZKIV010000000
VERSION     DAZKIV010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.112"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            177..419
                     /gene="yjbE"
                     /locus_tag="ACHOT6_003370"
     CDS             177..419
                     /gene="yjbE"
                     /locus_tag="ACHOT6_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFP9948534.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            533..1171
                     /gene="yjbF"
                     /locus_tag="ACHOT6_003371"
     CDS             533..1171
                     /gene="yjbF"
                     /locus_tag="ACHOT6_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFP9948535.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1168..1905
                     /gene="yjbG"
                     /locus_tag="ACHOT6_003372"
     CDS             1168..1905
                     /gene="yjbG"
                     /locus_tag="ACHOT6_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFP9948536.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1905..4001
                     /gene="yjbH"
                     /locus_tag="ACHOT6_003373"
     CDS             1905..4001
                     /gene="yjbH"
                     /locus_tag="ACHOT6_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFP9948537.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4048..4326)
                     /locus_tag="ACHOT6_003374"
     CDS             complement(4048..4326)
                     /locus_tag="ACHOT6_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948538.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4342..4470
                     /locus_tag="ACHOT6_003375"
     CDS             4342..4470
                     /locus_tag="ACHOT6_003375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948539.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            4596..5006
                     /gene="psiE"
                     /locus_tag="ACHOT6_003376"
     CDS             4596..5006
                     /gene="psiE"
                     /locus_tag="ACHOT6_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFP9948540.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5050..6525)
                     /gene="xylE"
                     /locus_tag="ACHOT6_003377"
     CDS             complement(5050..6525)
                     /gene="xylE"
                     /locus_tag="ACHOT6_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFP9948541.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRIIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6897..7787)
                     /gene="malG"
                     /locus_tag="ACHOT6_003378"
     CDS             complement(6897..7787)
                     /gene="malG"
                     /locus_tag="ACHOT6_003378"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFP9948542.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7802..9346)
                     /gene="malF"
                     /locus_tag="ACHOT6_003379"
     CDS             complement(7802..9346)
                     /gene="malF"
                     /locus_tag="ACHOT6_003379"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFP9948543.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPTGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTTQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPLQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9500..10690)
                     /gene="malE"
                     /locus_tag="ACHOT6_003380"
     CDS             complement(9500..10690)
                     /gene="malE"
                     /locus_tag="ACHOT6_003380"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFP9948544.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11055..12170
                     /gene="malK"
                     /locus_tag="ACHOT6_003381"
     CDS             11055..12170
                     /gene="malK"
                     /locus_tag="ACHOT6_003381"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFP9948545.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12242..13582
                     /gene="lamB"
                     /locus_tag="ACHOT6_003382"
     CDS             12242..13582
                     /gene="lamB"
                     /locus_tag="ACHOT6_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFP9948546.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTGNADTNANFGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13825..14745
                     /gene="malM"
                     /locus_tag="ACHOT6_003383"
     CDS             13825..14745
                     /gene="malM"
                     /locus_tag="ACHOT6_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFP9948547.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLVDVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14974..>15075
                     /locus_tag="ACHOT6_003384"
     CDS             14974..>15075
                     /locus_tag="ACHOT6_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948548.1"
                     /translation="MISHIPNSGGNNSLMKHDVVQGNNIVDLDLLRNF"
BASE COUNT         3740 a         3873 c         3944 g         3518 t
ORIGIN      
        1 atttttaatg ttatttatat ataagttgta gcttatattt gatattcatt ccaattatcc
       61 taaaacccca tctctaattc cccgcgccgt aattcgcatg ctttagttgt gtatactcga
      121 tcccgcccga aatgtttttg ggtaaatctc cattcattca atgaagggaa attgttatga
      181 aaaaagttct gtatggcatt tttgccatat ctgcgcttgc ggcgacttct gcgtgggctg
      241 cacctgtaca ggtgggcgaa gcggcagggt cggcagcaac gtcggtttcg gcggggagtt
      301 cctccgcgac cagcgtcagc accgtaagct cggcggtggg tgtcgcgctc gcggcaaccg
      361 gtggcggtga tggttctaat accgggacca ccacaaccac gaccaccagt acccagtaat
      421 aaagtatgta tccccaaaat aatttgagtc attgcatctg tggcttgaag tatgaaggga
      481 tttaccataa ccacactccg gtgtggttat tctgcccctc tggagaagag tcgtgaagcg
      541 acctgcactc attcttatct gcctgctatt acaggcctgt tcagccacga ctaaagagct
      601 gggcaattca ctgtgggaca gtctgttcgg cacgccaggc gtacagctga cggacgatga
      661 tattcaaaat atgccctacg ccagccagta catgcagctt aatggcgggc cgcaattgtt
      721 tgtggtgctg gccttcgctg aagacggaca acaaaaatgg gtcactcagg atcaagccac
      781 tcttgtcaca caacatggtc gtctggtgaa gactttgctt ggcggcgaca acctgattga
      841 agtgaataac cttgccgccg acccgctgat taaacccgca caaattgttg atggtgcaac
      901 atggacgcgc acgatgggct ggaccgagta ccagcaggta cgctacgcca ccgcacgctc
      961 agtcttcaaa tgggatggca ccgataccgt caaggtcggc agcgatgaaa ccccggttcg
     1021 cgtgctggac gaagaagtct ccaccgacca ggcgcgctgg cataaccgct actggatcga
     1081 cagcgaaggg caaattcgcc agtcggaaca gtatctcggc gcggattatt tcccggtgaa
     1141 aaccactctc atcaaggcgg caaaacaatg attaaacaaa ctattgtcgc attgattttg
     1201 agtgtggggg cgtcatcggt cttcgcggcg ggaaccgtca aggtattcag caatggcagc
     1261 agtgaggcca aaacgctgac gggcgcagag tatttaatcg atctggtagg ccagccgcgg
     1321 ctggcaaaca gttggtggcc tggtgcggtg attagcgaag agctggcaac ggcggcagca
     1381 ttgcgtcagc agcaggcgtt gctgacacgg ctggcagaac agggcgcaga ttccagcacc
     1441 gacgatgccg ccgccattaa cgccttacgc cagcaaatcc aggcattgaa agtgacaggc
     1501 aggcaaaaaa tcaatcttga tcccgatatc gtccgcgttg ccgaacgcgg taacccgccg
     1561 ttgcagggca actacacgct gtgggtcgga ccaccgccgt ccacggtcac gttgttcggg
     1621 cttatcagcc atcctggcaa tcagccattc actccaggtc gcgacgtggc gagctatctc
     1681 tctgaccaga gcctgctcag cggtgcggat cgcagctacg cgtgggtcgt ttacccggac
     1741 ggacgcacgc aaaaagcgcc ggtggcttac tggaacaagc gtcacgtaga gccgatgccc
     1801 ggcagcatta tttatgtagg cctcgcagac tccgtctgga gtgagacgcc tgatgccctt
     1861 aacgccgaca ttcttcagac tctgacgcag cggatacccc aataatgaaa aaaagacatc
     1921 tgcttagctt actggcgctg ggcattagca cagcttgcta cggcgaaaca tatcctgcgc
     1981 ccattggtcc gtcgcagtcg gatttcggtg gcgtaggatt attacaaact cccaccgcgc
     2041 gtatggcacg ggaaggggag ttgagtctga actatcgcga taacgatcag taccgttatt
     2101 actcagcttc agtgcaactc ttcccgtggc tggaaacaac gctgcgctac accgacgtgc
     2161 gcacccggca gtacagcagc gtcgaagcgt tctctggcga tcaaacgtat aaagataaag
     2221 ccttcgatct caaactgcgt ttgtgggaag agagttactg gctgccgcaa gtggcggttg
     2281 gtgcgaggga tatcggcggt acggggctgt ttgatgcgga atatcttgtt gccagcaaag
     2341 cctgggggcc gttcgatttt acgctcggcc tgggctgggg ttatctgggc accagcggta
     2401 atgtgaaaaa tccgctctgt tcagccagtg ataaatattg ctatcgcgat aacagctaca
     2461 aacaggcggg atctatcgac ggtagccaga tgttccacgg tcctgcctca ctgtttggcg
     2521 gcgtggaata ccagacgccc tggcaaccgc tgcgcctgaa actggagtat gaaggcaata
     2581 attatcagca ggattttgcc gggaagctgg agcaaaaaag taagtttaac gtcggtgcga
     2641 tttatcgcgt taccgattgg gccgacgtta accttagcta tgaacgtggc aacaccttta
     2701 tgtttggcgt cacgctgcgc accaacttta acgatctgcg cccgtcttac aacgataacg
     2761 cccgcccgca atatcaaccg cagccgcagg atgccatttt gcagcattcg gtggtggcga
     2821 atcagttaac gctgttgaaa tacaatgccg gacttgccga tccgcagatc caggcgaaag
     2881 gcgatacgct gtatgtcacc ggcgagcagg tgaaatatcg tgattcgcgc gaagggatca
     2941 tccgtgctaa tcggatcgtg atgaacgatc tgccggatgg gatcaaaacg atccgcatta
     3001 cggaaaatcg tcttaacatg ccgcaggtga cgacggaaac cgatgtcgcc agcctgaaaa
     3061 atcatctcgc cggagagccg ttgggccacg aaacgaagct ggcgcaaaaa cgcgtcgagc
     3121 cagtggttcc gcagtccacc gagcagggct ggtatatcga caaatcacgc tttgatttcc
     3181 atatcgatcc ggtgctgaac cagtcggtcg gtggcccgga aaacttttac atgtatcagc
     3241 tgggcgtgat gggaacggca gatttgtggc tgacggacca tctgctgacc accggcagcc
     3301 tgtttgcaaa tcttgccaac aactacgaca agtttaacta caccaatccg ccgcaggact
     3361 cgcacttacc gcgcgttcgt acccatgtgc gcgagtatgt gcagaacgat gtctatgtga
     3421 ataacctgca agccaactac ttccagcatc tgggcaacgg cttctacggt caggtctacg
     3481 gtggttatct cgaaaccatg tttggcggtg cgggggcaga agtgttgtat cgcccgctgg
     3541 acagcaactg ggcgtttggt ctggatgcca actacgttaa acagcgcgac tggcgtagtg
     3601 caaaagatat gatgaaattc accgactaca gcgtgaaaac cggacatctg accgcgtact
     3661 ggacgccatc tttcgctcag gatgtgttag tgaaagcgag cgtcgggcag tatctggcgg
     3721 gcgataaagg cggcacgctg gagatcgcca aacgctttga tagcggcgtg gtggttggcg
     3781 gctatgccac gatcaccaac gtttcgaaag aggagtacgg cgaaggggac ttcaccaaag
     3841 gcgtgtatgt ctcggtaccg ttggatctct tctcgtctgg cccgacacgc agccgtgcgg
     3901 cgattggctg gacgccgctg acgcgtgacg gtggtcagca acttgggcgt aagttccagt
     3961 tgtatgacat gaccagcgac cgtagcgtca atttccgcta agtcatggga aaggtgccag
     4021 ttttcgcaat cactggcacc ctcattctta atggcctgtt gagccatcag gaacgcagtt
     4081 cacccccggt ttgcgatctg gtgtgtaaag gcgaaaacat tctgtcttcc tgtccgtcga
     4141 tatcggttgc agatctggtt gcctgagcga ctgggccatc attgggctgg agacattgct
     4201 ttgcggtagg tcggctttat cagcagtacc gagcggacca gcataagcag gaagaacaga
     4261 gactgataac atcaaagcag caaaataagg cttcattttt accaccttta tcaggttacg
     4321 tttcatttgt tccagaggaa catggtcgat ttttcgcgca ttgctggtgg ctgggaatca
     4381 cctgaatggg tgatttttga attaccggct ttggtgcggt ttgtcttgcc ggatgcgccg
     4441 ccaggcgcgg cttatccggc ctacgggtag gtatatccgg ctttggtgga ggcgcgctcc
     4501 aaatccaggt tgaacaaaac atacacaaaa aatatagatc tccgtcacat ttttgcgtta
     4561 tacaggaagc tcgccactgt gaaggaggta ctgctatgac gtcactctct cgtccgcgcg
     4621 tggagtttat ctccaccatt ttgcagaccg tactcaatct tggcctgttg tgcctcggcc
     4681 tgattttggt tgtcttcctc ggcaaagaaa cggtgcatct ggcagatgtg ctgttcgcgc
     4741 cagaacaaac cagcaaatat gagctggtag aaggactggt ggtttacttt ctctatttcg
     4801 aatttatcgc gctgattgtg aaatactttc agtccggttt tcacttcccg ctacgttact
     4861 ttgtctatat tgggatcacc gccattgtgc gcttgatcat cgtcgatcat aaatcgccac
     4921 tggatgtgtt gatctactcg gctgcgatcc tgttactggt gatcactctg tggctgtgta
     4981 attcgaaacg gctgaagcgg gagtaaaaag acagcacgcc gaactggcgc ggcgtgctgg
     5041 acaggaagat tacagcgtag cagtttgttg tgttttcttc gtttccggtt cccagagggc
     5101 ttccagctcc tcaagggttt tacctttggt ttccgggaca aatttccaca taaatagtgc
     5161 tgccagaacg cccatacacc cgtaaatcca gtaggagaaa ccgttgtgga aatgggccac
     5221 cagccaggag tttttgtcca tcatcgggaa ggtccaggag acgaagtagt tcgccagcca
     5281 ctgggccgcc accgcgattg ccagcgcttt accacgaata gcattcggga agatttccga
     5341 cagcagtacc cagcataccg gaccccagga catggcaaag gcagcaacat agaacagcat
     5401 cgacagtagc gccacaatac ccggtgcctg agtgtaaaac gcggtaccga ggctaaacat
     5461 accgattgcc attccgagtg cgccgataat ttgcagtggc ttacgaccaa atttatccac
     5521 cgtcataatt gccagaacgg tgaaggtgag gttgataact ccgacaataa tggtctgcaa
     5581 cagcgcgata tccgtgctgg cccccagcgt tttgaacact tccggcgcgt agtacaacac
     5641 cacattgatg ccgacaaatt gctggaagat ggagagcata acgccgatta caatcacgcc
     5701 cacgccaaac atcagcagac gaccaccggt tttgcggcca tgatccaggg agtgtttaat
     5761 ttcctgtact gcctgagttg caagcgtgtt gcccataatt atgcgcagga tactttccgc
     5821 ctgttcttgc ttgccgcgtg acatcagcca gcgaggactt tctggcacgg tatacagcag
     5881 cattaagaac agcagtgcag ggatacattc cgaggcaaac atataacgcc agccgtcagt
     5941 attcagccag ctggcatcac cggaacgggc aataaaatag tttacgcagt aaactaaaag
     6001 ttgcccgaaa ataatcgcaa actggttaaa tgaaaccagt tttccgcgaa tatgagctgg
     6061 agccagttcc gcaatataca ttggtgaaag cattgaggct aaaccaacgc caataccgcc
     6121 aataatacga taaataacaa attccgggac ataacctgcc agataaatag gtactgtgtt
     6181 gtccgggttt atagaggtaa aaccaagttc tggccaggca gaacctacac cagaaataaa
     6241 aaacaggaca gcagcaatct taagtgaatc acgacggccg aagcggttac tgcaataacc
     6301 accgagggca ccgccgatga tgcaaccaat cagagcgctg gctacgcaaa atcctaacag
     6361 ggagttggca gcggattcac ttaagttttg tggagcaaca aagacggtat tgagtgactc
     6421 aacagtaccg gaaataacgg cggtgtcgta gccaaataat aaaccaccta atgtagcgac
     6481 taaggtaatc gaaaatatat aactggaatt atactgggta ttcattcaga cctgccttag
     6541 accattctga tcttttatca gtgcattgat gttggaattg tcaggcagtt aaggcaatta
     6601 tcatttttta caatgcgttt acgtaatgtc ttttctgtga tcttaattgt gataattatc
     6661 taaaattgat gaaaacaata ctattgccgt gactcagagc acgaaagaga attatcgtaa
     6721 gtgggaaaac aaataacgta aaaataataa gctctatgat ggaaatatta accggcgaac
     6781 gattcagatt gcagacgaaa gaaaaaaagg cgctccgtgg agcgccgaat aacagtcaca
     6841 agttgggata acgtaagttg agggtgcagc ggcataacat tggcagaaca acatctttaa
     6901 cctttcacac cacctgccgt caggccgttg accagccagc gttgagccag caagaagacg
     6961 atggtgatcg gtaatgcaga catcacggca gcggcggcaa agtcacccca caggtagttt
     7021 tgcgggttga ggtattgctg catccccacg gccagggtgt agctgtttac gtcacgcagt
     7081 aacagcgacg cgaccggaac ttcagtaatg gcagcgataa acgacaggat gaataccacc
     7141 gccagaatcg gtactgacag cggcaacagg acaaggcgga aggcctgcca cggtgtcgca
     7201 ccatccagcg cagccgcttc ttccagcgaa ctatcgatgg tttcgaaata gcctttgatg
     7261 gtccagacat gcagcgcaat cccacccaga tacgcgaaaa ttacgccgcc gtgagtattc
     7321 aggccaatga atggaatgta ctcacccaga cgatcaaaca acgcatacaa cgcgaccagt
     7381 gaaagtactg ccgggaacat ctggaaaatc agcattcctt tcagcagcgt cgctttgcct
     7441 ggaaagcgca tacgggcgaa agcgtaggcg caggtggtag agagcgccac aatgccaatc
     7501 gcggaaatcc cggcgacctt taccgagttc catagccaca gcagtaccgg gaatggcggt
     7561 ggcgtaatgc gaccatcagc ctgttccacg ctaaaaccta acgccagttt ccagtgatcc
     7621 caggagattt gctccgggat caggctgccg gtcgcaaagt tcccctggcg cagtgagata
     7681 gcgacgacca tcagcagcgg gaacataatc gccgcgataa aaagtagcag tagcaggtga
     7741 gtaataaaca aacgtgcttt ttgcgatttc ggttggacca ttgccatttt tgttatctcc
     7801 cttaatcaaa cttcattcgc gtggctttca ggttcactat cgccagcgca cccaccagca
     7861 ggaagatcag cgtggcaatt gctgccgcca gaccgaagtc ctgacccccg ccgccttcaa
     7921 aagcgatgcg gtaggtgtag ttaacaagca ggtcggtata accggctggc gtggtcgtgc
     7981 caagacgatc cgggccgccg ttggttaaca gttgaatcag cacgaagttg ttaaagttaa
     8041 aggcgaagct ggcgatcatc agcggcgtca gcggtttaat cagcagcggc agcgtaatct
     8101 taaagaagtt ctgcaacgga cctgcaccat ccattgctga ggcttcatac aaatcgtccg
     8161 gaatcgcttt cagcaagccc atgcagagga tcatcatgta cggataaccc agccaggtat
     8221 tgacgataat cagcatcgtg cgggcggtgg tcggatcgct gaaccaggcg ggcttcacgc
     8281 caaacagcgc gctcaacatc atgttgattt caccgaagct ctggttaaac aatcctttga
     8341 aaatcaagat tgaaatgaac gatggcaccg cgtagggcag aatcagcagg acgcgataga
     8401 ccgctttgcc gcgcaacgct tcccactgca ccagacatgc cagaaccatg ccgaccgcca
     8461 ccgttaaaaa gacagtgatc agcgagaaca ccacggtcca gacgaaaatg gcgaggaacg
     8521 gtttctgaat gccttcgtcg gtaaagacgc gggtaaagtt tttccagccg gtggtcacgg
     8581 tgtaaccggg gcttagcttt tcatcacccc agttgccgtc ggcggtaatg gactggtaaa
     8641 agccaatttg gttattcgga cgatatttca cgccgctctg attattcgtc aacgtgccgt
     8701 caccgtcgag tgtgtagagc ggctgcgtgc cagaaaactg gcgcagggag ctcatcatca
     8761 ctttgttgcc atccggcaga atggcggtaa tgtcactcag cgcctgacga ttctgggtaa
     8821 tcacgcgcag attcgcgcgt tcaccttcgg gctgggtggt cgtttctttc agttgcagtt
     8881 tttgttcgcc gccaaattta aacgcgtcgg aaaggtaatt tttgccggtt tcgccgtcgc
     8941 tgagcgccag ttgccactca tcgcccgtcg ggtaaagacc aaagttatag gttttgcctg
     9001 cttgccagga gcgatctaac aacacttcct gcgcacgttc aaaagtcagc tggttagtgc
     9061 tgctgtagtt ggtgaaggca atggcgatgg tgcagaccag agggaagagg acgaataatc
     9121 ccattccagc cattcccggg taaacatagc gccaggcgta ggctttacga ttggcgaaaa
     9181 tatacagccc cgctgaactc aatatcagcg tggtaatggc gaacaggtat tccccttgtg
     9241 cgtacattaa aacaacaagg taacccacca gcaggccgag cagacctagc actgaccatt
     9301 tcagcgcgtc gctttgccac caatgtttct ttttaatgac atccatgggg ttcttcctca
     9361 ttccaggacg gataaggctt tcacgcctta tccgacaaca actgcctgat gcgacactga
     9421 tgcgtcttat caggcctaca tgcgtttcag ttttgtaggc cggacaaggc gttcacgccg
     9481 catccggcat ttcatagcat tacttggtga tacgagtctg cgcgtctttc agggcttcat
     9541 cgacagtctg acgaccgctg gcggcgttga tcaccgcagt acgcacggca taccagaaag
     9601 cggacatctg cgggatgttc ggcatgattt cacctttctg ggcgttttcc atggtggcgg
     9661 caatacgtgg atctttcgcc aactcttcct cgtaagactt cagcgccact gcacccagcg
     9721 gtttgtcttt attgaccgct tccagacctt cgtcagtcag cagatagttt tcgaggaact
     9781 ctttcgccag ctctttgttc ggactggcgg cgttaatacc ggcgctcagc acgccaacga
     9841 acggtttaga tggttgaccc ttgaaggtcg gcagtaccgt tacaccataa ttcactttgc
     9901 tggtgtcgat gttggaccat gcccacgggc cgttgatggt catcgctgtt tcgcctttat
     9961 taaaggcagc ttctgcgatg gagtaatcgg tgtctgcatt catgtgtttg tttttaataa
    10021 ggtcaaccag gaaggtcaga cccgctttcg cgccagcgtt atccacgccc acgtctttaa
    10081 tatcgtactt gccgttttca tacttgaacg cataaccccc gtcagcagca atcagcggcc
    10141 aggtgaagta cggttcttgc aggttgaaca tcagcgcgct cttacctttc gctttcagtt
    10201 ctttatccag cgccgggatc tcttcccagg tttttggtgg gttcggcagc agatctttgt
    10261 tataaatcag cgataacgct tcaacagcga tcgggtaagc aatcagcttg ccgttgtaac
    10321 gtacggcatc ccaggtaaac ggatacagct tgtcctggaa cgctttgtcc ggggtgattt
    10381 cagccaacag gccagattga gcgtagccac caaaacggtc gtgcgcccag aagataatgt
    10441 cagggccatc gccagttgcc gcaacctgag ggaatttctc ttccagttta tccggatgct
    10501 caacggtgac tttaattccg gtatctttct cgaatttctt accgacttca gcgagaccgt
    10561 tatagccttt atcgccgtta atccagatta ccagtttacc ttcttcgatt ttggcgagag
    10621 ccgaggcgga aaacatcatc gtcgttaatg cggataatgc gaggatgcgt gcacctgttt
    10681 ttattttcat aatctatggt ccttgttggt gaagtgctcg tgaaaacacc taaacggact
    10741 ctagtttctt tatacggcaa cctctttcca tcctccttgc ccctacgccc caccgttgct
    10801 ttgtgtgatc tctgttacag aattggcggt aatgtgggga tgcgcacata aaatcgccac
    10861 gatttttgca agcaacatca cgaaattcct tacatgacct cggtttagtt cacagaagcc
    10921 gtgttctcat cctcccgcct cctcccccat aaaaaagcca gggggtggag gatttaagcc
    10981 atctcctgat gacgcatagt cagcccatca tgaatgttgc tgtcgatgac aggttgttac
    11041 aaagggagaa gggcatggcg agcgtacagc tgcaaaatgt aacgaaagcc tggggcgagg
    11101 tcgtggtatc gaaagatatc aatctcgata tccatgaagg tgaattcgtg gtgtttgtcg
    11161 gaccgtctgg ctgcggtaaa tcgactttac tgcgcatgat tgccgggctt gagacgatca
    11221 ccagcggcga cctgttcatc ggtgagaaac ggatgaatga cactccgcca gcagaacgcg
    11281 gcgttggtat ggtgtttcag tcttacgcgc tctatcccca cctgtcagta gcagaaaaca
    11341 tgtcatttgg cctgaaactg gcaggcgcaa aaaaagaggt gattaaccaa cgcgttaacc
    11401 aggtggcgga agtgctgcaa ctggcgcatt tgctggatcg caaaccgaaa gcgctctccg
    11461 gtggtcagcg tcagcgcgtg gcgattggcc gtacgctggt ggccgagcca agcgtatttt
    11521 tgctcgatga accgctctcc aacctcgatg ctgcactgcg tgtgcaaatg cgtatcgaaa
    11581 tctcccgtct gcataaacgc ctgggccgca caatgattta cgtcacccac gatcaggtcg
    11641 aagcgatgac gctggccgac aaaatcgtgg tgctggacgc cggtcgcgtg gcacaggttg
    11701 ggaaaccgct ggagctgtac cactatccgg cagaccgttt tgtcgccgga tttatcggtt
    11761 cgccaaagat gaacttcctg ccggtaaaag tgaccgccac cgcaatcgat caagtgcagg
    11821 tggagctgcc gatgccaaat cgtcagcaag tctggctgcc ggttgaaagc cgtgatgtcc
    11881 aggttggagc caatatgtcg ctgggtattc gcccggaaca tctactgccg agtgatatcg
    11941 ctgacgtcat ccttgagggt gaagttcagg tcgtcgagca actcggcaac gaaacccaaa
    12001 tccatatcca gatcccttcc attcgtcaaa acctggtgta ccgccagaac gacgtggtgt
    12061 tggtagaaga aggtgccaca ttcgctatcg gcctgccgcc agagcgttgc catctgttcc
    12121 gtgaggatgg cactgcatgt cgtcgactgc ataaggagcc gggcgtttaa gcaccccaca
    12181 aaacacacaa agcctgtcac aggtgatgtg aaaaaagaaa agcaatgact caggagatag
    12241 aatgatgatt actctgcgca aacttcctct ggcggttgcc gtcgcagcgg gcgtaatgtc
    12301 tgctcaggca atggctgttg atttccacgg ctatgcacgt tccggtattg gctggacagg
    12361 tagcggcggt gaacaacagt gtttccagac taccggtgct caaagtaaat accgtcttgg
    12421 caacgaatgt gaaacttatg ctgaattaaa attgggtcag gaagtgtgga aagagggcga
    12481 taagagcttc tatttcgaca ctaacgtggc ctattccgtc gcgcaacaga atgactggga
    12541 agctaccgat ccggccttcc gtgaagcaaa cgtgcagggt aaaaacctga tcgaatggct
    12601 gccaggttcc accatctggg caggtaagcg cttctaccaa cgtcatgacg ttcatatgat
    12661 cgacttctac tactgggata tttctggtcc tggtgccggt ctggaaaaca tcgatgttgg
    12721 cttcggtaaa ctctctctgg cagcaacccg ctcctctgaa gctggtggtt cttcctcttt
    12781 cgccagcaat aatatttatg actataccaa cgaaaccgcg aacgacgttt tcgacgtgcg
    12841 tttagcgcag atggaaatca acccgggcgg cacattagaa ctgggtgtcg actacggtcg
    12901 tgccaacctg cgtgataact atcgtctggt tgatggcgca tcgaaagacg gctggttatt
    12961 cactgctgaa catactcaga gtgtcctgaa gggctttaac aagtttgttg ttcagtacgc
    13021 tactgactcg atgacctcgc agggtaaagg tctgtcgcag ggttctggcg tcgcgtttga
    13081 taacgaaaaa tttgcctaca atatcaacaa caacggtcac atgctgcgta tcctcgacca
    13141 cggtgcgatc tccatgggcg acaactggga catgatgtac gtgggtatgt accaggatat
    13201 caactgggat aacgacaacg gcaccaagtg gtggaccgtc ggtattcgcc cgatgtacaa
    13261 atggacgcca atcatgagca ccgtgatgga aatcggctac gacaacgtcg aatcccagcg
    13321 caccggcgac aagaacaatc agtacaaaat taccctcgca caacaatggc aggctggcga
    13381 cagcatctgg tcacgcccgg ctattcgtgt cttcgcaacc tacgccaagt gggatgagaa
    13441 atggggttat gactacaccg gtaacgctga taccaacgcg aacttcggca aagccgttcc
    13501 tgctgatttc aacggcggca gcttcggtcg tggcgacagc gacgagtgga ccttcggtgc
    13561 ccagatggaa atctggtggt aatagcaaaa cctgggccgg ataaggtgct tgcaccgcat
    13621 tcggcaacca acgcctgatg cgacgcttgc gcgtcttatc aggcctacaa cggctgtcaa
    13681 atgtaggccg gataaggcgt ttacgccgca tccggcataa aaacaggttg tcattatctg
    13741 aaaggggcga aagcccctct gattatcggg tttagcgcgc tattgcctgg ctaccgctga
    13801 gctccagatt ttgaggtgaa aacaatgaaa atgaataaaa gtctcatcgc cctctgttta
    13861 tcagcagggt tactggcaag cgcgcctgga atcagccttg tcgatgttaa ctacgtaccg
    13921 caaaacacca gcgacgcgcc agccattcca tctgccgcgc tgcaacaact cacctggaca
    13981 ccggtcgatc aatctaaaac ccagaccacc caactggcga ccggcggcca acaactgaac
    14041 gttcccggca tcagtggtcc ggttgctgcg tacagcgtcc cggcaaacat tggcgaactg
    14101 accctgacgc tgaccagcga agtgaacaaa caaaccagcg tatttgcgcc gaacgtgctg
    14161 attcttgatc agaacatgac cccatcagcc ttcttcccca gcagttattt cacctaccag
    14221 gaaccaggcg tgatgagtgc agatcggctg gaaggcgtta tgcgcctgac accggcgttg
    14281 gggcagcaaa aactttatgt tctggtcttt accacggaaa aagatctcca gcagacgacc
    14341 caactgctcg acccggcaaa agcctatgcc aaaggcgttg gtaactcgat cccggatatt
    14401 cccgatccgg ttgctcgtca taccagcgat ggcttactga aactgaaagt caaaacgaac
    14461 tccagctcca gcgtgttggt aggaccacta tttggttctt ccgctccagc tccggttacg
    14521 gtaggcaaca cggcggcacc agctgtggct gcatccgctc cggcaccggt gaagaaaagc
    14581 gagccgatgc tcaacgacac ggaaagttat tttaataccg cgatcaaaaa cgctgtcgcg
    14641 aaaggtgatg ttgataaggc gttaaaactg cttgatgaag ctgaacgcct gggatcgaca
    14701 tctgcccgtt ccacctttat cagcagtgta aaaggcaagg ggtaattacg ccccacagtg
    14761 ctgattttgc aacaactggt gcgtctcctg gcgcaccttt ttttatgctt cattcctggg
    14821 atatgagcga ttttttatag taactcactt cttcttcact aagaatatcc attatctcaa
    14881 tgccttatca gagattcttt tcctttcgcc ggtagtgtct ggacgctcag gctacttttc
    14941 caggttattt tatttctgtt atgcagaggt tttatgataa gtcatatccc aaattctggc
    15001 ggcaataact ctttgatgaa acatgatgtg gtgcaaggaa ataatatagt agatcttgat
    15061 ttactacgta atttt
//
LOCUS       DAZKIV010000113        15023 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZKIV010000113 DAZKIV010000000
VERSION     DAZKIV010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.113"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(222..1274)
                     /gene="fbaB"
                     /locus_tag="ACHOT6_003385"
     CDS             complement(222..1274)
                     /gene="fbaB"
                     /locus_tag="ACHOT6_003385"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFP9948549.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            1531..2808
                     /locus_tag="ACHOT6_003386"
     CDS             1531..2808
                     /locus_tag="ACHOT6_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFP9948550.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGPACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            2805..3809
                     /locus_tag="ACHOT6_003387"
     CDS             2805..3809
                     /locus_tag="ACHOT6_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFP9948551.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPNLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPIAKLENNGVTNGAAMRVSPLGCLLPAHDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            3806..4771
                     /locus_tag="ACHOT6_003388"
     CDS             3806..4771
                     /locus_tag="ACHOT6_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFP9948552.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGAL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(4745..5491)
                     /locus_tag="ACHOT6_003389"
     CDS             complement(4745..5491)
                     /locus_tag="ACHOT6_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9948553.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(5543..6361)
                     /locus_tag="ACHOT6_003390"
     CDS             complement(5543..6361)
                     /locus_tag="ACHOT6_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFP9948554.1"
                     /translation="MQLRITSRKKFTALLCVLGLISIIAIYPRQTVNFFYSTAVQIKD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGMLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(6426..7226)
                     /gene="thiD"
                     /locus_tag="ACHOT6_003391"
     CDS             complement(6426..7226)
                     /gene="thiD"
                     /locus_tag="ACHOT6_003391"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFP9948555.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARNEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(7223..8011)
                     /gene="thiM"
                     /locus_tag="ACHOT6_003392"
     CDS             complement(7223..8011)
                     /gene="thiM"
                     /locus_tag="ACHOT6_003392"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFP9948556.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLVL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGVANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDML
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(8046..8142)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(8234..8506)
                     /gene="rcnR"
                     /locus_tag="ACHOT6_003393"
     CDS             complement(8234..8506)
                     /gene="rcnR"
                     /locus_tag="ACHOT6_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFP9948557.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQEDELKREEDLDVVLKVLDSYIK"
     gene            8627..9475
                     /gene="rcnA"
                     /locus_tag="ACHOT6_003394"
     CDS             8627..9475
                     /gene="rcnA"
                     /locus_tag="ACHOT6_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFP9948558.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHGHDYEHHHHHHDHEHHQDHEHHHDHGHHHHHEHGEYQDA
                     HARAHANDIKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLV
                     VSFSIGLALTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHG
                     FMGIMR"
     gene            9694..10032
                     /gene="rcnB"
                     /locus_tag="ACHOT6_003395"
     CDS             9694..10032
                     /gene="rcnB"
                     /locus_tag="ACHOT6_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFP9948559.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(10114..11148)
                     /locus_tag="ACHOT6_003396"
     CDS             complement(10114..11148)
                     /locus_tag="ACHOT6_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFP9948560.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPIITTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSPVTLLNITNSSKGYTNLKEVAAKSKLTDTTVSIPITASYYVYDTNKIKSGVLE
                     ATALINVKYD"
     gene            complement(11164..13644)
                     /locus_tag="ACHOT6_003397"
     CDS             complement(11164..13644)
                     /locus_tag="ACHOT6_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFP9948561.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSNYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSYSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVIYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(13660..14334)
                     /locus_tag="ACHOT6_003398"
     CDS             complement(13660..14334)
                     /locus_tag="ACHOT6_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9948562.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(14415..14957)
                     /locus_tag="ACHOT6_003399"
     CDS             complement(14415..14957)
                     /locus_tag="ACHOT6_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948563.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT         3731 a         3770 c         3486 g         4036 t
ORIGIN      
        1 aatatgaaaa ttgttcgagg agtgaaaggc aaaaaaacgg cctcccgata gggaagccgt
       61 agcaaagtgc gcgtgttttt atgccggatg cggtgtaaac atacgtggca gtgctatgta
      121 ggcatgataa gacgcttccg cgtcgcatca ggcataggtt gccggatgca gtgtaaacgc
      181 cttatccgtc ctacggggtg gtgttgttcg tataaggcgt atcaggcaat agtaattttg
      241 ctatcgagat aaacgtcctg cacggcgtta atcagtttca cgccgtcagc catcgatttc
      301 ttgaacgcct tacgtccaag aatcagcccc attccgccag cgcgtttgtt gataaccgca
      361 gtacgcactg catcactgag gtcagtttca ccgcctgcag caccgccgga gtttatcaac
      421 ccagcccggc ccatatagca gttagctaac tgataacgca ccagatcaat cgggttttcg
      481 ctggttaact tgctgtacac gcgatcgtcg gtataaccgt aattaatcgc tttatagccg
      541 ccgttatttt ccgccatttt ttgtttgacg atatctgcgc cgatggttgc cgccagatgg
      601 tttgcctgac cggtcaggtc ggcggaaaca tggtaatcaa cgccatcttt cttaaaggcg
      661 gagttacgca aataggccca cagcacggtc accatgccca gctcatgcgc acgttcaaaa
      721 gccgcagaaa tttcttcaat ctggcgacgt gactcttccg agccaaaata gatagtcgca
      781 ccaaccgcca ccgcgcccat gttgaaagcc tgctccacgc tggcatacag cgtttgatcg
      841 taggtattcg ggtaacttag cgtctcgttg tgattaagtt tgacgagaaa tggaatgcga
      901 tgcgcatagc gccgcgatac cgacgccaac acgccgtaag ttgatgccac acagttacag
      961 cccgcttcga tcgccagttc aacaatgttt ttcgggtcaa agtagagcgg gttagcagca
     1021 aatgaagctc cggcagagtg ctcaacgccc tggtcaaccg gcagaataga aagatatcct
     1081 gtgccagcca gacgcccggt gttgtacaac gtctgcatat tacgtaacac cgctggcggg
     1141 cgattattgt caatcattac gcggtctacg tagtcatgtc cggggagata aagctggtca
     1201 gaaggaattg tcatacaacg gtgctgtaaa aggttgtcgg cgtctttgcc aagcaactgc
     1261 gcaatatctg tcattactat gctcccgtaa attccgattg gatatcggct atggattgtc
     1321 ctggcccgcc ttttgcgggc aatcataatc ctggtcgtta cgggcgagtt tttccatctt
     1381 ttggatgttt tttcagcgtt ttctgttggc tcgattcatc agaaaaaatg ttagcgcggt
     1441 caatttttca ccacaaaggt atttaaaagg tattattaag tggtattgtc atcgcgtacc
     1501 ttacattacc tgtcatgaag gaattaaaag atgaaaacaa cagcaaagct gtcgttcatg
     1561 atgtttgttg aatggtttat ctggggcgcg tggtttgtgc cattgtggtt gtggttaagt
     1621 aaaagcggtt ttagtgccgg agaaattggc tggtcgtatg cctgtaccgc cattgcggcg
     1681 atcctgtcgc caattctggt tggctccatc actgaccgct ttttctcggc gcaaaaagtg
     1741 ctggcggtat tgatgttcgc aggcgcgctg ctgatgtatt tcgctgcgca acagaccact
     1801 tttgccgggt tctttccgtt actgctggct tactctctaa cctatatgcc gaccattgcg
     1861 ctgactaaca gcatcgcttt tgccaacgtg ccggatgtgg agcgtgattt cccgcgcatt
     1921 cgtgtgatgg gcactatcgg ctggattgcc tccggtccgg catgtggttt cttgccgcaa
     1981 atactggggt atgccgatat ctcaccgact aacatcccgc tgctgattac cgccggaagt
     2041 tctgctctgc tcggtgtgtt tgcgtttttc ctgcccgaca cgccaccaaa aagcaccggc
     2101 aaaatggata ttaaagtcat gctcggcctg gatgcgctga tcctgctgcg cgataaaaac
     2161 ttcctcgtct ttttcttctg ttcattcctg tttgcgatgc cactggcgtt ctattacatc
     2221 tttgccaacg gttatctgac cgaagttggc atgaaaaacg ccaccggctg gatgacgctc
     2281 ggccagttct ctgaaatatt ctttatgctg gcattaccgt ttttcactaa acgctttggt
     2341 atcaaaaagg tattattgct tggtctggtc accgctgcga tccgctatgg cttctttatt
     2401 tacggtagtg cggatgaata tttcacctac gcgttactgt tcctcggtat tttgcttcac
     2461 ggcgtaagtt acgattttta ctacgttacc gcttacatct atgtcgataa aaaagccccc
     2521 gtgcatatgc gtaccgccgc gcaggggctg atcacgctct gctgccaggg cttcggcagt
     2581 ttgctcggct atcgtcttgg cggtgtgatg atggaaaaga tgttcgctta tcaggaaccg
     2641 gtaaacggac tgactttcaa ctggtccggg atgtggactt ttggcgcggt gatgattgcc
     2701 attatcgccg tgctgttcat gatttttttc cgcgaatccg acaacgaaat tacggctatc
     2761 aaggtcgatg atcgcgatat tgcgttgaca caaggggaag ttaaatgaaa acagaacgta
     2821 ttctcggtgc tctttatggg caggcgttag gggatgcgat ggggatgccc tccgagcttt
     2881 ggccacgcag ccgcgttaaa gcacactttg gctggattga ccgttttctt cctggaccaa
     2941 aggagaataa cgcggcctgt tattttaacc gcgccgaatt caccgacgat acctcgatgg
     3001 cgctgtgtct ggcggatgcg ttactggaac gtgaaggcaa gatcgatccg aatctgattg
     3061 ggcgtaatat tctcgactgg gcgctgcgtt tcgacgcctt taacaaaaac gtacttggtc
     3121 cgacctcgaa gattgcgctt aacgccattc gcgacggtaa acccattgct aaactggaaa
     3181 acaacggcgt gaccaacggc gcagcgatgc gcgtctcgcc attaggttgt ttgcttccgg
     3241 cgcacgatgt tgattccttt attgatgatg tggcgctggc gtccagcccg acccataaat
     3301 ccgatctggc ggttgcaggc gcggtagtca tcgcatgggc gatttctcgt gccattgacg
     3361 gagaaagctg gtcagcgatt gtagattcac tgccttcaat tgcgcgacat gcccagcaaa
     3421 aacgcatcac taccttcagc gcctcactgg cagcacgtct ggagattgcg ctgaaaattg
     3481 tgcgcaatgc cgacggcacc gaatccgcca gcgaacagct ttaccaggtc gttggcgcag
     3541 gtaccagcac tattgagtcc gttccgtgcg ccattgcgct ggttgaactg gcacaaaccg
     3601 acccgaatcg ctgcgccgtc ctgtgcgcta accttggcgg cgacacagac accatcggcg
     3661 ctatggcgac ggcgatttgc ggcgcgttgc atggcgttaa cgctatcgat cctgcattaa
     3721 aggcggaact ggatgcggta aatcagcttg atttcaaccg ctatgccaca gcgctggcga
     3781 aatatcgtca acaacgggag gcggtatgag cggcgctcga ttacacacgc tgctgcctga
     3841 attaacctcg cgtcagccgg tgatggtcgt cggcgcggcg gtcattgatg tgatcgccga
     3901 cgcttatgcc ctcccctggc gtgggtgcga tatcgaactg aagcagcaga gcgttaacgt
     3961 tggcggctgc gcactgaata ttgccgtggc gttaaagcgc ctcggcatcg aagcgggtaa
     4021 tgccttgccg ctcggtcagg gcgtgtgggc ggagattatt cgcaaccgga tggcgaaaga
     4081 ggggttaatc agcctgatcg ataacgccga aggcgataac ggctggtgtc tggcgctggt
     4141 tgagccggat ggcgaacgca cttttatgtc attcagcggt gtggaaaatc agtggaatcg
     4201 ccagtggctg gcgcgattaa ccgttgcgcc tggcagcctg ctctattttt ccggttatca
     4261 actggcctcg ccctgcggcg aattgttagt ggaatggctg gaaaagctgc aagacgtgac
     4321 gccgtttatc gattttggcc cacgtatcgg cgatatcccg gacgcattac tggcgcggat
     4381 catggcctgt cgacctttag tatcactcaa tcgtcaagag gctgagattg ccgccgaacg
     4441 ttttgcttta tccgcagaga taacaacact tggcaagcaa tggcaggaga aatttgccgc
     4501 gccgttgatc gttcgtctcg ataaagaggg tgcatggtat ttcagcaacg acgcttctgg
     4561 ctgcattccg gcatttccaa cgcaagttgt agacaccatt ggggcgggcg acagtcatgc
     4621 aggtggcgca cttgccgggc tggcctctgg tctgccactg gcggatgccg tattactggg
     4681 caatgcagtg gcgtcgtggg ttgtcgggca tcggggcggt gattgtgcgc caacgcgcga
     4741 ggaactactc ctcgcacaca aaaacgtata gatcgctgcg acagtggcta atgctgtact
     4801 caataggccg ttgctgttgg tcaagcgcca cttgcttgat caccagcacc ggtattttgc
     4861 tatctaactg aatatgtgac tgaaactcgg catccggcat ccgggcgcta acgcgggaac
     4921 gtgtacgttg tgggtaaatg tgctggctgc ggaaatagtc ataaagtgaa atcccgatgg
     4981 catcaacatc atgaattaaa tgcgccggaa cccacgattc ctcaatcgat accgcttctt
     5041 catcgacata gcgaatacgc ttaagcaaaa agacatcact tcctgcttcg accgccagct
     5101 gctgcgcgac ttcttcggga catttcacga cacgcttgtt aacccatagc gtatcgggct
     5161 ttttcccgcg caataccacc tgctgagaaa aacctcgcgc ttctttcaac gagtattcga
     5221 agatgttgtt gatctgcgtg ccgtaaccgc gcgaacgcgt caccacaccc tcttcttcca
     5281 gcgcctgcat cgccttgcgc accgtaatgc gcgaaacgcc ggttaactga cttaaatcac
     5341 gctcaccggg caaaatattg ccatgctcca gcaccccgct gcgcacggca ttttttaccg
     5401 tttcggcaaa cttaatataa agcggcgtgt tatccgcagc taaaatacgc tcgttcagtt
     5461 gggcaatcag ctgggtatgc gcttgttcca tttgtctttt tcctgccagg gagtctggtg
     5521 ggagtatatc actgcttctt atttaaggcg tttctttaat cccatcaacg aatccctgca
     5581 actcctccac cgtgccatta aacacattaa aatccaccgg accattgatg ccatctacct
     5641 gtccacggtc ggaatgctgc cagaagcgcc aggccattcc gtcattgtcc ggacgacgtt
     5701 gataatagtg agccacccac cacggatatt cattaaaata gcccgccaga ttcgtgtgat
     5761 aaaaaacggc tcctgagtaa ataatcggct tttttcccgt gcgtttttct accattttta
     5821 gccactgact tacccgcttg cgtaattctt ttgccgataa ttttccccgt tcttctacgt
     5881 ccagcacggc agggaaatcg ccttgtgaga aatccaccgt ttgcagaaat aatcgcgcct
     5941 gaactgaagc ggataccgac ggggagaaat aatgatacgc cccacgcagc atgccatttt
     6001 cgcggcttag ttgccagtta cgcgaaaaat aggggtccac cagcttttcg ccttccgtcg
     6061 ccttaataaa agcaaactgt aagcggatac cgttgtcgcg catttttgcc acccgctgcc
     6121 agtcgatccg ctcctgccag cgcgaaacat ctattccgtg aatggtataa ctggcaggaa
     6181 tgcgaattgc gaaagattta accgggcgat aaccgtagaa gtggatgtag tctttaatct
     6241 gaactgctgt cgaatagaaa aaattcaccg tctgacgcgg ataaattgca atgatggaaa
     6301 ttagcccaag aacgcataat aaagcggtga atttttttcg actggtgatt ctcagctgca
     6361 ttggtttcat ccctgaatgt cagtgccaaa gactgacaat aaccaaagca gctatagtac
     6421 ggtgctcacc accaggcgtg gaagtggtga accggaccaa taccgtgacc aacttccagc
     6481 gtgtcggcct gggctaacgc cgatgagagc cagctttttg cctcctgtac ggtgtcagcc
     6541 cagtttgtat ggcgcgggcg tagtgcagcc aacgccgcag agagtgtaca accagtgccg
     6601 tgggtgtttt tggtcataat gcgcggtgcg gtaaaccgtt gttcaccctc gcgagtaaac
     6661 agccagtccg ggctttgctc atcatccaga tgaccacctt tcattagcac tgcgccacag
     6721 cccatcgcca acagcgatcg cccttgttcc agcatttcct gttcgttgcg cgcgtgtggc
     6781 gcgtcgagca aggcggcagc ttcgggcaag tttggcgtta ttaatgaaac ctgtggcaat
     6841 aatcgactgc gcagcgtagc aaccgccgaa ggtgaaagca gcggatcgcc gctttttgcc
     6901 agcataacag tgtcgagtac cacgttttgg atctgataac gttgcaaccg ttctgccacc
     6961 gcttcaacaa tatcggtttc cgccaacata ccgattttag tggtatcgat tcgcacatcg
     7021 ctgaacaccg aatcgagctg ggcggcgaca aaatcaggct caatgcgata caccgactgt
     7081 acgccacggg tattttgcgc caccagtgca gtaataactg agcaaccata agcgccaagt
     7141 gccgagaagg ttttaagatc ggcctgaatc cccgcaccac cactcggatc agtaccggca
     7201 atcgtcagag cgttaattcg tttcatgcct gcacctcctg cgtcaattgc cagagcgcat
     7261 caaggaaatg tggaacaaaa ctgcctggcc cctcgcttct ggcgacagcg cgttctccgg
     7321 cttgtttcat ccagtgacag gcagatgcga cattttccag catatcgcct ggtaaggcac
     7381 aacaggcagc gacaaccgcc gataatgcac agccagttcc taccactttg gtcattaacg
     7441 gatcgccacc gtgaatacca atgatacgat gtccatcggt aacataatcc acctcgccag
     7501 tgaccacgac gattgcgcca gtttcccgtg ccagtgtttg tgcagcgggt atcgcgttag
     7561 ctgcggcgtc agtggtatcc actccccgtc cgccattagc aacgccagct aatgccatga
     7621 tttccgaagc attaccacgt atcgctgccg gtttaaaaga taaaagttca tgacaaaaat
     7681 ggcggcgata atcgagcgca ccaaccgcta ctggatcaag cgtccagggt gtttgagagc
     7741 tttttgcttg ctcaacggca gcacgcatcg cctgtgcgcg tggctgcgtc agtgtgccaa
     7801 cgttaatcaa caaggcactg gcgatagccg caaactgact ggcctcttcg gtttcgataa
     7861 ccatcgctgg cgatgcaccg agcaccagca aggtattggc agtaaaggtt tgcaccacat
     7921 cgttggtcat gcagtgcaca agaggggaat gttggtgaaa aaggtgtaac gcgtgcgcag
     7981 attgcgctga actcagcagg tcgacttgca tagtttgctc ctgccataac gtgaagaagc
     8041 aatgacctgg tggtccgtga cttccctacg ctggcattat ccagatcagg tgatacgggt
     8101 atttctcagc cttcacgcag aagggcaccc cgagtcgttt ggttgcgatg ataaatcgca
     8161 gaggaggatg gtaatgtcca gcgcacccgt tgtaaacgag gaaaaacggc agttttacaa
     8221 tagcgttaat aaattatttg atatatgaat ccagcacctt cagaacgaca tccagatctt
     8281 cttcacgttt tagctcatcc tcctggtgaa cgatgtgttc cgtcagatga cctttaatca
     8341 cttcccgcat cagaccgttt accgcgccac ggatagcagc aatctgttgt aaaactgcag
     8401 cgcattcgtg cggctcgtcg agcattttct tgagcgccac gacctggccc tgaatcttac
     8461 tggcacgcgc tttcagtttc tgtttatcac ggattgtatg agacatggca acacctggtt
     8521 aacaagaata tgaaaaatca tagcactatt aatctactgg ggggtagtat caggtactgg
     8581 gggggagtag aatcagattg ccgaattaat actaagaatt attatcatga ccgaatttac
     8641 aactcttctt cagcaaggaa acgcctggtt cttcatcccc agcgccatct tacttggtgc
     8701 gcttcatggc ctggaaccag ggcactcaaa aacgatgatg gcagcgttta tcatcgccat
     8761 caaaggcacc attaaacagg cggtgatgct cggactggca gcaaccattt cgcataccgc
     8821 agtggtctgg ttgattgcct ttggcgggat ggtgatcagc aagcgcttta ctgctcaatc
     8881 agcagaaccg tggctccagc tgatttccgc agtgatcatt attggcaccg cattctggat
     8941 gttctggcgt acctggcgcg gcgaacgcaa ctggctggag aatatgcacg ggcatgatta
     9001 tgagcatcat caccatcatc acgatcacga acatcatcaa gaccacgaac atcaccacga
     9061 ccatggacat catcaccatc acgaacatgg cgagtatcag gatgcccatg cacgagccca
     9121 tgccaatgat attaaacgac gctttgatgg tagagaggtc accaactggc aaattttgtt
     9181 atttggctta acaggtggtc ttatcccctg tccggcggca attaccgtgc tgttgatttg
     9241 cattcagttg aaagccctga cactgggcgc aacactggtc gtcagtttca gcattggcct
     9301 ggcgttaacg ctggtcaccg taggcgttgg cgcagcaatc agcgttcagc aggtcgcaaa
     9361 acgctggagc ggatttaaca ctctcgctaa acgcgccccc tatttttcca gtctgttgat
     9421 tggcttagtt ggtgtgtata tgggcgtaca tggcttcatg ggcataatgc gataactcaa
     9481 tcctttaggc ttgcatgtta tcttcagccc aggatgattc ttaaatcagc tatttccgct
     9541 gactctctgc tcgctaagga tgcttttagg gcattctttc atttacactt tttacgaaat
     9601 catgggatca ctaacaaaat atcgcttgtc agttatattg tatggcagga aagatatgcg
     9661 actgatatta cagatcccca aagtggagag tttatgacca ttaaaaataa gatgttgctg
     9721 ggtgcgcttt tgctggttac cagtgccgcc tgggccgcac cagccaccgc gggttcgacc
     9781 aatacctcgg gaatttctaa gtatgagtta agcagtttca ttgctgactt taagcatttc
     9841 aaaccaggcg ataccgtccc agagatgtac cgcaccgatg agtacaacat taagcagtgg
     9901 cagttacgta acctgcccgc gcctgatgcc gggacgcact ggacctatat gggtggcgcg
     9961 tacgtgttga tcagcgacac cgacggtaaa atcattaaag cctacgacgg tgagattttt
    10021 tatcatcgct aaaaaaagcc ccctcatcat gagggggaaa tgcagacaca ttgttatttt
    10081 ttattattag ccacttgctc gtcttgcttg ttattagtcg tatttcacgt tgattaatgc
    10141 ggttgcctcc agtacgccag atttaatttt atttgtatcg tagacgtagt aactggcagt
    10201 tatcggaatt gaaaccgttg tatctgtaag ttttgacttc gccgcaactt cctttaaatt
    10261 tgtatatcct ttactgctgt tagtgatatt taaaagtgtg actggcgaac cattactttc
    10321 aagaataaat cctacgccat tatcttccgc acctttcagt attgtgttgc tgttggttaa
    10381 caagtcagaa cttgataaat acacctgcat tttccgtgat gtttgatttt gttgacctaa
    10441 taaattttta caacgcagaa taatatcccc ttgtttacta tttattgttg ctttgttacc
    10501 tgtggctggt aattgtgaaa cgggaatact gtctaaagta attaataaat cgtccggttt
    10561 gcaggttgtg attatcgggt tgtatgacaa tgtcagatta aaggaataga cacctgcatt
    10621 ttgtgccaat gcatttctgt agctatttac acatgcatcc catgacttgc cacctaataa
    10681 acacagtaga aattgtactg cggcactccc gcgtgtcgat gatgaagcac tggataatgt
    10741 cgcaacacca ttaatcatca ctgaaccgcc gccccccgca gaaacattca cttttgcatt
    10801 actggccgca ggtgtcaccg taactgaata accactggtg aaactggtct gattatttga
    10861 caacgtgatg ttgtttttat tggtccattc aaaatgtaaa tttaatttta tggtatcgtt
    10921 ataaagtcca acaataatat ccgacgggct cataggggtg atagtatttg tacttacgca
    10981 tgacgaatca ttaaaattat gctcatatgt cgccatttgt gtttgagtaa gtgcaaaatc
    11041 cccctgaaca accaagttgt gctgaacaga taactcacta taacatgccg catagctcac
    11101 aggcataacc atcatcatta atataaatag cattatgcga atttccatag aaccacctga
    11161 ctattactgg caaatataat ttctactttc atcaatctct ttaccgaagg tgattgtgca
    11221 tgaaagtcct tgttgcttat caattgccac attaaccgat ggcggtactt cattggtgcg
    11281 aataaataac tggcttccct ggccgacaac gccaatatta tgaccatgga tatcattgac
    11341 ttcataacca aacattaatg gttgaccatc cgctcttaac gctttaataa accagggctt
    11401 gcgctgatcg gtatcaaaat taaccagtac aaccgcgccg cgataagggg cggcaatttt
    11461 ccggttgcca cgtaattctg cttcgctatc gctttgcgac acatccagca tcaggtgatt
    11521 ttcccgataa ggtgtcattc cgtcgtatat caccactcca ttacggtttg ttgtgcgata
    11581 tttttgccca ttgacataag catctttaat tcctggcgca ttcatcacag caaacgtttc
    11641 ggaaagacgg ttcgccagat taacgccacc cgaccaggcg acaatgcccc ctgaaacact
    11701 ggctccagcc tgtcgataag tactcgactg actataactg ccattcactg tcgcaaccgg
    11761 cgcgttccag gtcaaattcg ccccagctgt cgtttcattt ccctgatgct gataactcag
    11821 gttgacccca tagttaaact gatcccggct tccaacggtt cctgataatc ccgtattatt
    11881 tgaggcaaac ccctgatcat caaacgtcgt tgagttagac atatatattt gccgacgagg
    11941 cgtcgtaacg tcatcacccc aatcaaaggg aatcgatata aaaatattaa aacgtttctc
    12001 ttcatgatga ttctcgtcat acgcctggct tgccgcgagg gtatagctta tccgccgcca
    12061 gttattggaa taactcaact gataatcctt actgctgccg ctacgccccc agtaatctcg
    12121 ccataacgta cttaaggaca cagaccccca gccttctggc aatgactggc tcatattggc
    12181 agaaaagcta tttttacggc caaaatcgtt ctggtaataa tcggcaatgt catagatatc
    12241 gttttcatca cggcgataat tatctttatt gtttgcccaa acgtgatcat taaatgtccg
    12301 gtaatcacgc gatgaataac gccaggccgc aagaccaaaa cgcgtcgacg tttggctcac
    12361 aaatttgttg taggcaattt gataactttg cccgtcaaac acatcgccgt tgtcttgttt
    12421 actatacgac ttcgtggcat cgacggaaat ggcaccaatg cgtgtattcc agccagtccc
    12481 caaagtaaac gcgtaataat tattcgcgac catcgagcca ccatacagcg tcaataaatt
    12541 attaaaacca tactgataac ccgcctggac aaaatcactt tgtttgctcg ccccttcaat
    12601 atggctacga cccgccgcaa aatcataatt cgacacgccg ggttgcagca tatttggcac
    12661 cgctgcataa ggcaccagat aggtggttac cgagccgtcc gcctctttca cgctgacatc
    12721 aagatctgct ccaccaccgg ccaactgcaa atctgtaatc gcaaacgggc caggaggaac
    12781 ctctttctga taaaccacaa aaccattctg ttcaatagtt accagcgcgt tactctgagc
    12841 aatcccctgc acccgtggcg taaaattttg tttcgagtta ggcaacatct gcatatcacg
    12901 aaacaaccgc acaccgctga agcgaacaga atcaaaaata tcgcttgatg tgtacatatc
    12961 acccacgcga agcgtgccga gaagttgggc aaatccacgt tccagataca gggtattgct
    13021 tttccacacc cctggattgt tatttgtttt actgaaactg gcatcagaat gcagttgcca
    13081 ctccagtaaa tttaacccgc tgttaaaacg tacatatgta ctcttgttgt tacccgacgc
    13141 tttatagtcg ctgtaatact gactcacata ataagaggta taaaacgcat taataccccg
    13201 ctcccagttt tccggtggaa catagccact ttccagttct tccacccaag cctgcgggac
    13261 actgaaatcg agacgaaaaa ccccgatatc ccagctatag ctcccaccct gaacaagttg
    13321 ctcaaatgtt aaacattgct taccgctggc gaagttatcg ctattaatgc ctaaccgctt
    13381 gataacttct cttgataaac atgtttcttg cgggttgtct ttaacaataa tctcatattt
    13441 cccgcgccat tgcttattga cataaatatc gatgtcatac tgccccggta agggttgatt
    13501 atcatcaaga cgaatatttg caacctgctg gtctttcatt ccacctatca taaaatgggt
    13561 atcaaaggtt tcttcagctg cataagcttc aatgccgagc aataacgcta cgattgctga
    13621 tgcaagtggg gtcattctca acataggcag ctcctgcaat taaattttgt cactaatata
    13681 gttgccgtgg tcatcgataa tggtcagata ccagttattt gcattattac ttttgacatt
    13741 aacactctga ctttctaagg gggcaatcat tatagtttca taattgactt tgacattatt
    13801 agctttgaca tccgaaatcg tcacccaatt agctgagtca ttttttatca ccaatccatt
    13861 gccactgcga tttaccagta actttttaaa ggtcgcttta tttaccggag caatacccgc
    13921 cggccggtaa aacaatttga ttctgttttg catcgcaaac ttcagtgcat tcttaccttc
    13981 ttgttctgga ctatttggtg gaatatccag aacattcaga taaaaaatgc tttctttatt
    14041 agtgggcagt ttattcggca taattttgat ttttacttgc tgcccggaat ttgcccctat
    14101 ttttgccact ggtggcgtta acatgaaagg aacctgaatt ttttctggtg gtaacgacgt
    14161 atcgccatca tcaatccacg cctgcagcag cgaagaacga tttccctggt tcatcaactg
    14221 caccataact tctttatttt ctgccgggta aataatgcgc gtcccgtaga taacaattcc
    14281 ggcatgtgca ggaaggacca tagaaaaaat gagtaaagat aataatcctt tcatcttaat
    14341 gcctcttaaa ttaaaaagcc gccatgggga tagcggccat gtttattacc tggagaaact
    14401 tgcaatttta tttattaatc ctgcgccaca cgcataacca gagaagataa cacatcacct
    14461 gattgcactg agttatctgt cagtttcagg tattttgctt taaacggaat agcatattga
    14521 ccttccgctg ttttataact ctcaatgtta aaaggtgcag aaacatccag gacatcgctg
    14581 ttatcaccta aatagagata gtaacccacg gtatcgttgc tactggctat cgccttatcg
    14641 ttaccggtta ttttatcgtt ggtaatcaac gagagtttta caccagcaac ttctttcggg
    14701 cagctaattt ttaacatgct gctggttaca ccgactgcat catcataggt agaatattta
    14761 gctttacctt caaagacagt ggttggtaca gtcggcatat taatacttac tgaatcacca
    14821 cccgcttcga atttacacgg agattctgca atattcccct taatagttaa tgttccggta
    14881 tcaacgtctg cagcaaatac tccagcgctc ataaaaaaag aagaaaagac agctgcagca
    14941 ataattgaac gtttcataat atgttcctta ataaaattta tttttggttg ttaagagtct
    15001 gtatctcttg gaaacaaatt tct
//
LOCUS       DAZKIV010000114        14679 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZKIV010000114 DAZKIV010000000
VERSION     DAZKIV010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.114"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..479)
                     /locus_tag="ACHOT6_003400"
     CDS             complement(9..479)
                     /locus_tag="ACHOT6_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948564.1"
                     /translation="MTKSWSVPFPESETEHDGMPVFWRFQATVEEDGIKIFALQYIAF
                     HQTEHYAWLVPAHWIVNFKPAPNQWLQEWKQRRNRYAIKKVAKNAERSFAFPTKKLAI
                     ESLLRRKKYHLMRIKQDLAVVSTLVDGMKNIDTSTPDIEYNFGHNQETENWVFY"
     gene            complement(538..606)
                     /locus_tag="ACHOT6_003401"
                     /pseudo
     CDS             complement(538..606)
                     /locus_tag="ACHOT6_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(1393..1797)
                     /locus_tag="ACHOT6_003402"
     CDS             complement(1393..1797)
                     /locus_tag="ACHOT6_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948565.1"
                     /translation="MKIELISKKVERLVMTRLEGDSTAKKAIKTTVNLNNELYTNVKD
                     SKLFRVRYFASVTIEGRLEMDITYDFDFRSEDDFSHEMAKSYEVRSIAPNMAYPYIKT
                     YAEQIILMSNLGRFTLPYFDFLANPMETNSNK"
     gene            complement(1794..2450)
                     /locus_tag="ACHOT6_003403"
     CDS             complement(1794..2450)
                     /locus_tag="ACHOT6_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigA family
                     antitoxin"
                     /protein_id="HFP9948566.1"
                     /translation="MNRTSWRIIKNSEEHAAAMERLIELASSDLQPGTEDFDEFELLG
                     LLIEHYESREFPMDKPDPIEAIKFRMDQQGLSYADMKQYIGSASKVSEVLNRKRPLSL
                     SMIRRLHDGLGIPADILIQDMSAIEWSLVDAEEEETAMTSVIARCESAVTSPSAYFAE
                     KATESYFSKMLFSAVRGNGKCKEKRNVFSLISNLSSSFTAASNLNDEMTSDGNYLLLP
                     "
     gene            complement(2447..2734)
                     /locus_tag="ACHOT6_003404"
     CDS             complement(2447..2734)
                     /locus_tag="ACHOT6_003404"
                     /inference="COORDINATES: protein motif:HMM:NF021431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HFP9948567.1"
                     /translation="MKVLNVEKLHSFSRKHNQAKGALDSWYDEVIRENWKTTQDIRNR
                     FNSADFLPNNRVIFNIKGNNYRLVVQVVYQAGMVIVERVGTHAEYDKWRLK"
     gene            complement(2871..3575)
                     /locus_tag="ACHOT6_003405"
     CDS             complement(2871..3575)
                     /locus_tag="ACHOT6_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9948568.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            3689..3922
                     /locus_tag="ACHOT6_003406"
     CDS             3689..3922
                     /locus_tag="ACHOT6_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9948569.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            4061..4357
                     /locus_tag="ACHOT6_003407"
     CDS             4061..4357
                     /locus_tag="ACHOT6_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFP9948570.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDEITKKKSPVAAGDSKQIDMQF"
     gene            4390..5328
                     /locus_tag="ACHOT6_003408"
     CDS             4390..5328
                     /locus_tag="ACHOT6_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9948571.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNEAKLELVRMNIIKQQ
                     GGMFGPNKNTSEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSLKLGDCYPSKQGDTK
                     DTITKEKRKDYSSENSGESSDQTENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMV
                     KTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRD
                     KWTQLEINRNKQQAVVTASKPKLDLTNTDWIYGVDL"
     gene            5325..6026
                     /locus_tag="ACHOT6_003409"
     CDS             5325..6026
                     /locus_tag="ACHOT6_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9948572.1"
                     /translation="MKNIAAQMVNFDREQLRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQDRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            6023..6313
                     /locus_tag="ACHOT6_003410"
     CDS             6023..6313
                     /locus_tag="ACHOT6_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9948573.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            6384..6662
                     /locus_tag="ACHOT6_003411"
     CDS             6384..6662
                     /locus_tag="ACHOT6_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948574.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            6795..7010
                     /locus_tag="ACHOT6_003412"
     CDS             6795..7010
                     /locus_tag="ACHOT6_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948575.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            7021..7257
                     /locus_tag="ACHOT6_003413"
     CDS             7021..7257
                     /locus_tag="ACHOT6_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFP9948576.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            7214..7660
                     /locus_tag="ACHOT6_003414"
     CDS             7214..7660
                     /locus_tag="ACHOT6_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9948577.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            7657..8184
                     /locus_tag="ACHOT6_003415"
     CDS             7657..8184
                     /locus_tag="ACHOT6_003415"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9948578.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            8181..8363
                     /locus_tag="ACHOT6_003416"
     CDS             8181..8363
                     /locus_tag="ACHOT6_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9948579.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            8638..9372
                     /locus_tag="ACHOT6_003417"
     CDS             8638..9372
                     /locus_tag="ACHOT6_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFP9948580.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVFEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            9447..10169
                     /locus_tag="ACHOT6_003418"
     CDS             9447..10169
                     /locus_tag="ACHOT6_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFP9948581.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTREAA"
     gene            10169..10774
                     /locus_tag="ACHOT6_003419"
     CDS             10169..10774
                     /locus_tag="ACHOT6_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9948582.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            10771..10965
                     /locus_tag="ACHOT6_003420"
     CDS             10771..10965
                     /locus_tag="ACHOT6_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFP9948583.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            10958..11392
                     /locus_tag="ACHOT6_003421"
     CDS             10958..11392
                     /locus_tag="ACHOT6_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9948584.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            11899..12846
                     /gene="stxA1a"
                     /locus_tag="ACHOT6_003422"
     CDS             11899..12846
                     /gene="stxA1a"
                     /locus_tag="ACHOT6_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9948585.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            12856..13125
                     /gene="stxB1a"
                     /locus_tag="ACHOT6_003423"
     CDS             12856..13125
                     /gene="stxB1a"
                     /locus_tag="ACHOT6_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9948586.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            13625..>14679
                     /locus_tag="ACHOT6_003424"
     CDS             13625..>14679
                     /locus_tag="ACHOT6_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9948587.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMTDENG
                     VNVPTNKPEEDPDIPGIG"
BASE COUNT         4054 a         3209 c         3632 g         3784 t
ORIGIN      
        1 tattcgtact aataaaatac ccaattttct gtttcttggt tgtgtccaaa gttatattca
       61 atatctggtg ttgatgtatc aatattcttc ataccatcaa caagagttga tacaacagcc
      121 aaatcttgtt tgattctcat taaatggtat ttcttccggc gcaataaact ttcaatggca
      181 agtttcttcg ttgggaatgc aaaagatctt tctgcatttt ttgctacttt cttaattgca
      241 tatctatttc tcctttgttt ccattcctgt aaccactgat ttggtgctgg tttaaaatta
      301 acaatccaat gcgcaggaac caaccatgca taatgctctg tctgatgaaa agctatatat
      361 tgaagtgcga atatttttat cccatcttct tcaactgtcg cctggaatct ccagaaaaca
      421 ggcattccat catgttcagt ttctgattca ggaaaaggta cgctccatga ttttgtcata
      481 tatcacctca aataagtggt ttgctgccta atttcatttt ctggcgacca acacaagtca
      541 tcttgctgtc agttgtttgg atttccggta gcctgccgcg taaagagcta catttggaag
      601 acatacacca gtttctggtt gcttatgtcc aaactcattc gcgtacacaa tggccgctcg
      661 ctccagattg cgtctgtatt cttcctgctg ccagatcacg tcctgtgcca tgaacttaat
      721 tggcttagcg tcttctatgc gatcaggcgt ttcgtgagta cctttagcct gaatctgcgc
      781 tctacttaga gttgggcggt gtaatacttc tgaacttatt gcttcttcgc gggccagtac
      841 gccgttagct aatgcctttg cctttaaacg ctcacgacga cgagaacgag aattgccttt
      901 gaactgagtt ctgcgtgtca tatggaacct cctgatgaac tttggcggtg aatacagccg
      961 ggcgactaac tccggtcgcg taatcattgc aaagcgcctc cgccgagaag gttagcttct
     1021 gcattcaccc caaagttcac tttggttatt gcgctttgtc agcgccgtag attcatattc
     1081 gaatcgttgt atattcaccg ccctggtgag tagtgcgtcc tgctgatggg tgaactttat
     1141 cggaatgata aattaatggc aatagcaaaa tgataaattc tcttggtttt caaatatcgt
     1201 attgattctt atggtgtttt attttgttgt aggaattcga caggataaat aaagagattt
     1261 gagggagatc tggattgcgt tgtttagcaa gttgtatcta tttatttttc aacaaataca
     1321 attggttatg tgtttttagg tgtggggatc gtgaggcaaa gaaaacccgg cgctgaggcc
     1381 gggtggggaa tttcacttat tactgttcgt ttccatggga ttggccaaga aatcaaaata
     1441 aggcagagtg aacctaccaa ggtttgacat gaggataatt tgctcggcat atgtttttat
     1501 atagggatat gccatattgg gagcaatgga ccttacctca tatgattttg ccatttcatg
     1561 agaaaaatca tcttctgacc ggaagtcaaa gtcgtatgtg atgtccatct caagcctgcc
     1621 ttcaatagtt acggaggcaa aatatctcac tctaaatagc tttgaatcct tcacattagt
     1681 gtaaagttca ttatttagat taacggttgt ttttatggct ttttttgctg ttgaatcgcc
     1741 ttctagccgt gtcataacca agcgttcaac ttttttgcta atgagttcaa ttttcatggt
     1801 aataataagt aatttccgtc agaagtcatt tcgtcattca ggttgctagc cgctgtgaaa
     1861 cttgatgaca agttacttat caaagaaaaa acattccgtt tttctttgca tttgccatta
     1921 ccccttactg cgctgaacaa catttttgaa aagtaagatt ctgtagcctt ttcagcgaaa
     1981 taagcagaag gtgatgtgac ggctgactca caccgagcaa tgacgctagt catggctgtt
     2041 tcttcttcct ctgcgtcaac taggctccat tcaattgcgc tcatatcttg aattaagata
     2101 tctgcaggaa ttccaagtcc gtcatgtagt ctacggatca ttgaaagact taatggacgc
     2161 ttacgattta agacctcaga tactttagat gctgagccaa tgtattgttt catatcggca
     2221 taagagaggc cttgttgatc catacggaac ttgattgctt ctatgggatc tggcttgtcc
     2281 atagggaact cgcgtgactc atagtgctcg ataagcaagc ctagtagttc aaactcatca
     2341 aaatcttcag ttccaggttg taaatcacta gacgcaagtt caatgagcct ttccatagct
     2401 gcagcatgct cttcactatt tttaatgatg cgccagctag ttcgattcat ttaagcctcc
     2461 acttgtcgta ctctgcatga gttccaactc tttcaactat gaccattcct gcctggtaaa
     2521 caacttggac aacgagccga tagttattgc cttttatatt aaaaattacc ctgttgttag
     2581 gaaggaagtc ggcagaatta aatctattcc gtatgtcttg agtcgttttc cagttttctc
     2641 ttatcacttc atcataccaa gagtctaaag cccccttggc ctgattgtgc ttccggctaa
     2701 aactgtgaag cttctctacg tttaagacct tcataatccg attttgttcc ctctttggga
     2761 attatagatt catttcccat aaagggaatc tgtattttta tccagaaatg ctatgtacat
     2821 cacccaaacg tctcttcagg ccactggtta ccagctatgt gacgatgaag tcacgaactt
     2881 ttcagccact cccttgcctc gatgtcatcc agatggcgag attgcttcag aataccagcc
     2941 acatactcca cctttgctac ttgatgataa ggcaacgtta ttggcctgtg gtcctggttg
     3001 atgcttgtaa attggtattc tccgtctctg tcatagccaa gaactttgat catgttgtgt
     3061 ccttcaacag ttctgacaaa cacctcatca cccgggaata ctttggtgtt aggctcaatg
     3121 agtacatatt ctcctgattt tattctgggc cacatgctgt ctcctttcac acgaagacca
     3181 aaggcatctg gatcatcgct ataaattttg agccacccat cgcgctcttc ggtcatctcg
     3241 atggcaccat caacaccaag aattgcctca ccaaccacgc gcactaaccc ttttcttacc
     3301 tgaccgacaa aagttaaaga atctgagcat gatgcaattg gtgttacatc atgtaccaaa
     3361 tcaagccacc cattaggtaa cccaagcgcg gcttcaaatt ttcttgctag tttatcccct
     3421 atgtttcgag tgcttttttc accggagact tgcgtgagtt gagaagggct aaccccaagc
     3481 ttatcggcaa agcttgcatt agtgttaccc gcgatttttt tatgctcatc tagcaaaaac
     3541 gccagattcg atttgcgaat atctttgttt tccatctcac aattttccct ctatttagca
     3601 aatggataaa tacgcaatat gataaattta cattgcggat gatttatcaa aatggtaaag
     3661 ttgttctgtg tgataaacgg aggcactaat gagtaatgaa ctactacgct ggcgaaaaga
     3721 ggcttctagt gaggaatgga agcgactcgc cgcattagcg aaaacttcag ttggctatct
     3781 tgaccagatt gcatatggat ttcgaagagc ttcccctgat aaagcgaatg caatcgaaga
     3841 agctactcgt aatttcacgg gttataaacc tgtgaaaaag gaaaacctag tgttcgtatc
     3901 gcgtagagca tcggctgcat aagtaacacc gctattttca caatggacat tcgtcctacg
     3961 tcgctgacaa agcgagtccc aatatatctg accaactaag gccatatgcg tttccacgca
     4021 tacctttcaa ctagctattc actattggaa atcttaagaa atggaacaaa caagttacag
     4081 caaactatca cagcgagaaa ttgatcgcgc tgaaactgat ttactcatca acctgtcaac
     4141 gcttacccag cgcggtctgg caaagatgat tggctgtcat gaatcgaaga taagcagaac
     4201 agactggagg tttatagctt cggtcttgtg tgcttttggc atggcatcag acatcagtcc
     4261 gattagcaga gcttttaagt atgcgcttga tgaaatcaca aagaaaaaat ccccggtggc
     4321 cgccggggac tctaagcaaa ttgatatgca attctgaggg aattactgga tcaatccaca
     4381 ggagtcatta tgacaaatac agcaaaaata ctcaacttcg gcagaggtaa ctttgccgaa
     4441 caggagcgta atgtggcaga tctcgatgat ggttacgcca gactatcaaa tatgctgctt
     4501 gaggcttatt caggcgcaga tctgaccaag cgacagttta aagtgctgct tgccattctg
     4561 cgtaaaacct atgggtggaa taaaccaatg gacagaatca ccgattctca acttagcgag
     4621 attacaaagt tacccgtcaa acggtgcaat gaagccaagt tagaactcgt cagaatgaat
     4681 attatcaagc agcaaggcgg catgtttgga ccaaataaaa acacctcaga atggtgcatc
     4741 cctcaaaacg agggaggttc ccctaaaatg agggacatcc ctcaaaacga gggaaaatcc
     4801 cctaaaacga gggataaaac atccctcaaa ttaggggatt gctatccctc aaaacagggg
     4861 gacacaaaag acactattac aaaagaaaaa agaaaagatt attcgtccga gaattctggc
     4921 gaatcctctg accagacaga aaacgatctt tctgtggtta aaccggatgc tgcaattcag
     4981 agcggcagca agtggggaac agcagaagac ctgaccgccg cagagtggat gtttgacatg
     5041 gtgaagacca tcgcaccatc agccagaaaa ccgaattttg ctgggtgggc taacgatatc
     5101 cgcctgatgc gtgaacgtga cggacgtaac caccgcgaca tgtgcgtgct gttccgctgg
     5161 gcatgccagg acaacttctg gtccggtaac gtgctgagcc cggccaaact ccgcgacaag
     5221 tggacccagc tcgaaatcaa ccgtaacaag caacaggcag tcgtgacagc cagcaaacca
     5281 aaactcgacc tgacaaacac agactggatt tacggggtgg atctatgaaa aacatcgccg
     5341 cacagatggt taactttgac cgtgagcagt tgcgtcggat cgccaacaac atgccggaac
     5401 agtacgacga aaagccgcag gtacagcagg tagcgcagat catcaacggt gtgttcagcc
     5461 agttactggc aactttcccg gcgagtctgg ctaaccgtga ccagaacgaa ctgaacgaaa
     5521 tccgccgcca gtgggttctg gctttccggg aaaacgggat caccacaatg gaacaggtta
     5581 acgcaggaat gcgcgtagcc cgtcggcagg atcgaccatt tctgccatca cccgggcagt
     5641 ttgttgcatg gtgccgggaa gaagcatccg ttatcgccgg actgccaaac gtcagcgagc
     5701 tggttgatat ggtttacgag tattgccgga agcgaggcct gtatccggat gcggagtctt
     5761 atccgtggaa atcaaacgcg cactactggc tggttaccaa cctgtatcag aacatgcggg
     5821 ccaatgcgct tactgatgcg gaattacggc gcaaggctgc cgatgaactg acctgtatga
     5881 cagcacgaat taaccgtggt gagacgatac ctgaaccagt aaaacaactt cctgtcatgg
     5941 gcggcagacc tctaaatcga gcacaggctc tggcgaagat cgcagaaatt aaagctaagt
     6001 tcggactgaa aggagcaagt gtatgacggg caaagaggca attattcatt acctcgggac
     6061 gcataagaac ttctgtgcac aggacgtttc cgcggtaaca ggcgcaaccg taaccagcat
     6121 aaatcaggct gcggctaaaa tggcgcgggc aggaatcctg gtcattgatg gtaaggtctg
     6181 gcgaacggtg tattaccggt ttgctaccag ggaagaacgg gaaggaaaga tgagcacgaa
     6241 cctgattttt aaggagtgtc gccagagtgc agcgatgaaa cgggtattgg cggtatatgg
     6301 agttaaaaga tgaccatcta catcactgag ctaataacag gcctgctggt aatcgcaggc
     6361 ctttttattt gggggagagg gaagtgaacg atagctaccg acagtttgaa aactggtggt
     6421 caaaagacaa aagccagttc acgggagacg atgaattaaa agagtttgcc tgggtgatat
     6481 ggcaggcatc gcgctctgct attgaactgg atatcgactg gcccgaatcg aatgacgact
     6541 tttggaaaga tggtgaagaa ggtgcttatg cgatgggtta tgaggatggg cgtgacaaaa
     6601 cggtaattgc agtaatgaaa gccatcaggg ccgcaggaat caaagaaaag aatttcgatt
     6661 aagcaaatat cacttcaata aatcgctttt aaggcatcac aatcgctctg tagcgaggta
     6721 aacgcgtgca aggcatgcca ataagcagcg agaatgaaaa atgcgtcaga atgcgtttga
     6781 ggaggtttta agaaatgagt acgatagctg agcttgtcag ggctaatttt cgtgaagagt
     6841 tggtgcgttg gtatcggtat cgttcatcgt ccagtttgcc gcttgatgag ttgtatgagc
     6901 attcacctgc cgcacgacgc tatccgcgtg accgtgttct tcgacggttg ttcaaactca
     6961 acaatgagtt tcagcgcaac agaattatcc ggagtctgga tttaaagtga aggagtgagc
     7021 atgagcgacc tatcattaac ccagccaaag ctaaaagaat gtccgttttg cggcggtaat
     7081 gctcgtctgt gggttgaggc cggaataaat attgatgtgt ggggctatgc agaatgtgac
     7141 ctctgtgaag ccaggggggc atgggcacca tcagttgctg cggcggctga aaaatggaac
     7201 cggagagcag gagatgaagc aaacctttct gcttcgcaac gaagcaatca gaaataacgc
     7261 catagacgcc attctctcac tacccatcga cgacaagtca ccccacgaag tccacgttaa
     7321 agaacccaag cgcagcaaag cgcagaatga ccgtatgtgg ccgatgctga acgatgtttc
     7381 gcgtcaggtg ctatggcatg gtcaacggct ggcgccggaa gactggaaag acctgttcac
     7441 tgccctgtgg cttaagacca aaaaactgga gcaacgaagt gtgcctggta tcgacggtgg
     7501 cgttgtcatg cttggcgtgc gtaccagcaa aatgcggaag gccagcatga ctgagcttat
     7561 cgaaatcatg ttctggttcg gctcagagcg caacgtgcgg tggagtgatg actcccggcg
     7621 agagtatgaa tggtcacaac gaaaaggtag ggctgcatga ctatcaaatc aaatacgcca
     7681 gcacacgaca aggactgctg gcaaacgccg ctttggcttt ttgatgcact ggatattgag
     7741 tttggattct ggctggattc ggcagcgagc gacaaaaatg ctctgtgcgc tcactggcta
     7801 actgaggccg acgacgcgct caattctgag tgggtaagcc acggtgcaat ctggaataac
     7861 ccaccgtaca gcaatatcag gccgtgggtg gaaaaagccg ctgagcagtg catacaacag
     7921 cgacagacgg tagttatgct tgtgccagag gatatgtcag tcggatggtt cagcaaggct
     7981 ctggagagtg ttgacgaagt tcgcatcatc actgatggac ggattaattt tatcgaacca
     8041 tcgacagggc tggagaagaa gggaaacagc aaaggctcca tgctgctgat ttggcgaccg
     8101 ttcatcagtc ctcgacgaat gtttactact gtatccaaag cggcattgat ggcgatcggg
     8161 cagggcgtca ggagggcggc atgaggcgac agcgacgaag tatcaccgac atcatctgcg
     8221 aaaactgcaa ataccttcca acgaaacgct ccagaaataa acgcaagcca atcccaaaag
     8281 aatctgacgt aaaaaccttc aattacacgg ctcacctgtg ggatatccgg tggctaagac
     8341 atcgtgcgag gaaaacaagg tgattgacgc gatgatttat tcggggctat attcctcacg
     8401 cgccagcaaa atctggcgtc gggattggcg tcctggatag agaccgcgac agatacacgc
     8461 cgcgagcgtg ttttttattg tcgtatgcac gcgcacatct gaattatggt gggctgtgtg
     8521 ggggcggaga gatccgcgcc ggtcggtttc ccggttacgc caaccctgca cagttcacca
     8581 ccagacgatt ggcgtcgtcg gtggtgagtt attaagaaac caccagaggg cgtcattatg
     8641 acaactcaaa tttctgttga aactctctcc ccgatcaccc ataaccagat tcctgttatt
     8701 accaccgaac ttttggcgca gctttacggc actgagccgg tgcgtattcg ccagaatcat
     8761 catgagaaca aagtacgctt cgttgaaggg aaacactttt tcaaagttgt tggtaatgac
     8821 cttaaagaat tgcgggtagc tttaaactac tcacaaaatt tgcgggttac tttaagtaac
     8881 tcacaaaatt tgcaaccatc tttaagaggg ttacaaattt ccccgaaagc ccgctccctc
     8941 atactctgga cagaacgagg cgcagcccgt cacgcaaaaa tgctcgaaac tgatcaggcg
     9001 tgggatgtgt tcgaaaaact ggaagactgc tatttcagcc agtgcgagaa aaatactggc
     9061 aaacaagaga agaagctcaa cgggctttcc gcaaaagaaa cagacagcct tgtatggctg
     9121 tgggattatg ccaaccgctc acaggcattg ttccgtgagt tgtatcccgc attaaaactg
     9181 attcagtctg gctattccgg catatgccac gactacggct atgagttctc gtatatcatc
     9241 gggagggcga ggggcgtttt aattaatcac acgcgggata tagatattta tgagcctgac
     9301 gggccgacga accttctggc atgggaaagg cttaagaaca aagagttgcc gccttcactg
     9361 catcgctact gacaattgac aacttaacaa acccagcttc ggctgggttt tttattgctg
     9421 aattttcaat gtgagaggac atgacaatga atgagctgat aaatagcaat gccatcaaaa
     9481 tgacaagcat tgaaatcgct gagttggtgg gaagccgtca tgacaaggtg aaacaatcca
     9541 ttgaacgact ggcggttcga ggtgtgatcc gaaatccccc aatggtggtt ttcgaaaaaa
     9601 tcaataactt aggattactt cgtggcgtag aggcttacgt ttttgagggc gaacaaggta
     9661 agcgcgacag cattattgtc gttgcccagt tgtcgccgga attcaccgct cgccttgttg
     9721 accgctggcg agaactcgaa ggggcaaccg cgaaaatacc acaaaccttt tctgaggcat
     9781 tgcgccttgc ggccgacctt gaagaccaga aggctgaact ggagaaacag cttgctctcg
     9841 cagcacctaa agttgagttt gccgatcgcg ttggcgaggc cagcggaatt ttgattggaa
     9901 actttgcaaa ggttgttggt attggtccaa acaaactgtt tgcgtggatg cgcgatcaca
     9961 aaatccttat tgcttcaggc tcccggcgca atgtgccaat gcaggaatat atggatcgtg
    10021 gctatttcac agtgaaagaa acagcggtca atacaaatca cggaatacag atatcgttca
    10081 ccacaaaaat caccgggcgt ggtcaacagt ggctgaccag aaagctgctc gataacggaa
    10141 tgctgaaagt aacaagggag gctgcttaat ggctaaacca gcgcgaagga aatgcaaaat
    10201 atgcaaggaa tggtttcacc cggcattctc aaatcagtgg tggtgctgcc cggaacacgg
    10261 aactcaatta gcactcgaac gacgaagtaa agaacgcgaa aaagcggaaa aagcagcaga
    10321 gaagaaacga cgacgagagg agcagaaaca gaaagataaa ctgaagattc gaaaactcgc
    10381 cttaaagccc cgcagttact ggattaaaca agcccaacaa gccgtaaacg ccttcatcag
    10441 agaaagagac cgcgacttac catgtatctc gtgcggaacg ctcacgtctg ctcagtggga
    10501 tgccggacat taccggacaa ctgctgcgac acctcaactc cgatttgatg aacgcaatat
    10561 tcacaagcaa tgcgtggtgt gcaaccagca caaaagcgga aatctcgttc cgtatcgcgt
    10621 cgaactgatt aaccgcatcg ggcaggaagc agtagacgaa atcgaatcga accataaccg
    10681 ccatcgctgg actgtcgaag aatgcagggc gatcaaggcg aagtatcagc agaaacttaa
    10741 agacctgcga aacagcagaa gtgaggccgc atgacgttca cagtaaaaac cattcctgac
    10801 atgctccttg aggcatatgg aaatcagacc gaggtagccc gaatactgaa ctgcaatcgt
    10861 gccacagtca gaaaatacat tggcgataaa gaagggaaaa agcacgccgt cgtcaatggc
    10921 gtccttatgg ttcatcgcgg atggggtaaa gatactgatg cgtgatatcc ggcaggttct
    10981 tgagcgctgg ggggcatggg cggcaaataa ccatgaggat gttacatggt cgcccattgc
    11041 tgccggattt aagggactga tccccgaaaa agtaaaatca cgtccacagt gttgtgacga
    11101 tgacgcgatg attatatgcg ggtgtatggc tcgccttaac aggaacaaca gcgatctgca
    11161 tgacttgctg gttgattatt acgtgttggg ggagacgttc atggcgctgg cacggaaaca
    11221 tgggtgctct gacacctgta taggtaaacg ccttcacaaa gcggagggga ttgttgaagg
    11281 catgctgatg atgctgggag tgaggcttga gatggatcgg tatgttgagc gtgaattgcc
    11341 gggagggaga acctctgtat tttatcagcg aaaaaatagt ttacgatcgt aaaaatctgc
    11401 atatcatgat aagagtggtt acattgccac gctgcttaac ccgccgatgc gcgggttttt
    11461 ttgtacccag aatcctgtga gctatacgga aagtacacag aaaggaaggt gcgaccacaa
    11521 ttaataacaa aatcttaaaa attgcacatg gcactattag ttttctaaat attgtgtatt
    11581 ttttgtattg caggatgacc ctgtaacgaa gtttgcgtaa cagcattttg ctctacgagt
    11641 ttgccagcct cccccagtgg ctggcttttt tatgtccgta acatcctgtg tatcaataaa
    11701 tgttgttatc tacgtacgtc aagtagtcgc atgagatctg accagatatg ttaaggttgc
    11761 agctctcttt gaatatgatt atcattttca ttacgttatt gttacgttta tccggtgcgc
    11821 cgtaaaacgc cgtccttcag ggcgtggagg atgtcaagaa tatagttatc gtatggtgct
    11881 caaggagtat tgtgtaatat gaaaataatt atttttagag tgctaacttt tttctttgtt
    11941 atcttttcag ttaatgtggt ggcgaaggaa tttaccttag acttctcgac tgcaaagacg
    12001 tatgtagatt cgctgaatgt cattcgctct gcaataggta ctccattaca gactatttca
    12061 tcaggaggta cgtctttact gatgattgat agtggcacag gggataattt gtttgcagtt
    12121 gatgtcagag ggatagatcc agaggaaggg cggtttaata atctacggct tattgttgaa
    12181 cgaaataatt tatatgtgac aggatttgtt aacaggacaa ataatgtttt ttatcgcttt
    12241 gctgattttt cacatgttac ctttccaggt acaacagcgg ttacattgtc tggtgacagt
    12301 agctatacca cgttacagcg tgttgcaggg atcagtcgta cggggatgca gataaatcgc
    12361 cattcgttga ctacttctta tctggattta atgtcgcata gtggaacctc actgacgcag
    12421 tctgtggcaa gagcgatgtt acggtttgtt actgtgacag ctgaagcttt acgttttcgg
    12481 caaatacaga ggggatttcg tacaacactg gatgatctca gtgggcgttc ttatgtaatg
    12541 actgctgaag atgttgatct tacattgaac tggggaaggt tgagtagcgt cctgcctgac
    12601 tatcatggac aagactctgt tcgtgtagga agaatttctt ttggaagcat taatgcaatt
    12661 ctgggaagcg tggcattaat actgaattgt catcatcatg catcgcgagt tgccagaatg
    12721 gcatctgatg agtttccttc tatgtgtccg gcagatggaa gagtccgtgg gattacgcac
    12781 aataaaatat tgtgggattc atccactctg ggggcaattc tgatgcgcag aactattagc
    12841 agttgagggg gtaaaatgaa aaaaacatta ttaatagctg catcgctttc atttttttca
    12901 gcaagtgcgc tggcgacgcc tgattgtgta actggaaagg tggagtatac aaaatataat
    12961 gatgacgata cctttacagt taaagtgggt gataaagaat tatttaccaa cagatggaat
    13021 cttcagtctc ttcttctcag tgcgcaaatt acggggatga ctgtaaccat taaaactaat
    13081 gcctgtcata atggaggggg attcagcgaa gttatttttc gttgactcag aatagctcag
    13141 tgaaaatagc aggcggagat tcataaatgt taaatacatc tcaattcagt cagttgttgc
    13201 cggtctgata atagatgtgt tagaaaattt ctgcatggtg aatccccctg tgcggagggg
    13261 cgactggtga acggtatgat ctctttgatg atcgtaagcg agaatacgcg ggtttggtgg
    13321 caccaggccg aactcaccgg gaggcacccg gcatcatgct gtatacagag attaggcata
    13381 tatccaggct cctcatcgca ggagcctttt tacatgcaaa aaaaagcccg agtgggttcg
    13441 ggcaacagca tgagatactt gcattgtcat ttttatcgtg cggattttaa ccaggattca
    13501 taaggctgcg caactgcgcg gcctttttcg tatttcgggc tgtagtcccc gtgtgtcatt
    13561 caggcttccg gactacagcc cactccatat ctgatttaat acactatccc ggccgggagg
    13621 aataatgaca tttaaacatt atgatgttgt cagggcggcg tcgccgtcag accttgcgga
    13681 aaagctgaca cacaaactga aagagggctg gcagccgttt ggtagtccgg tggccataac
    13741 cccttatacc ctgatgcagg ccattgcggc ggaaggtgat gtcaccacac cagtggcggt
    13801 gaccggtaat gagggtaagg cggtggctgt cagtgccacc agcgacccgg agtattactt
    13861 tgttgtggtt ctggcagggc agtcaaatgg catgtcgtat ggtgaaggtc ttccgctgcc
    13921 ggagacatat gaccgtccgg acccgcgtat taagcagctg gcgcgccgca gtacggtgac
    13981 accgggcggt gcagcatgca aatataacga catcattccg gcggaccatt gtctgcatga
    14041 tgtgcaggac atgagccgtc ttaaccatcc gaaagcggac ctgtcaaaag ggcagtacgg
    14101 taccgtgggg caggggctgc atatcgccaa aaaactgctg ccgtttatac cggcgaatgc
    14161 gggcattctg ctggttccgt gctgtcgtgg tggttcagcg ttcaccaccg gagccgatgg
    14221 cacatacagt gacgcgagtg gtgcctcgga gaattcaacc cgctggggtg tggacaagcc
    14281 gctgtataag gaccttatcg gtcgaacaaa agcagcactg aagaagaatc cgaaaaatgt
    14341 gctgtttgcc gtggtgtgga tgcaggggga atttgatttt ggcggtacgc cggcaaatca
    14401 cgcagcacag tttggtgcgc tggttgataa attccgtgca gacctggcgg atatggcagg
    14461 tcagtgcgtc ggtggctctg ctggcggtgt tccctggatc tgtggagata cgacgtattt
    14521 ctggaagcag aagaacgaat cctcgtacca gacggtgtac ggcagctaca aaaacaaaac
    14581 ggaaaagaat atccatttcg taccgttcat gaccgatgag aacggggtga atgtgccgac
    14641 gaacaaaccg gaagaagacc cggacattcc gggtatcgg
//
LOCUS       DAZKIV010000115        14563 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZKIV010000115 DAZKIV010000000
VERSION     DAZKIV010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.115"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..428
                     /locus_tag="ACHOT6_003425"
     CDS             72..428
                     /locus_tag="ACHOT6_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9948588.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFCSN"
     gene            complement(479..1375)
                     /gene="yhaJ"
                     /locus_tag="ACHOT6_003426"
     CDS             complement(479..1375)
                     /gene="yhaJ"
                     /locus_tag="ACHOT6_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFP9948589.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1480..2181
                     /locus_tag="ACHOT6_003427"
     CDS             1480..2181
                     /locus_tag="ACHOT6_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFP9948590.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2204..2368
                     /gene="yhaL"
                     /locus_tag="ACHOT6_003428"
     CDS             2204..2368
                     /gene="yhaL"
                     /locus_tag="ACHOT6_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFP9948591.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2580..3890)
                     /gene="cyuA"
                     /locus_tag="ACHOT6_003429"
     CDS             complement(2580..3890)
                     /gene="cyuA"
                     /locus_tag="ACHOT6_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFP9948592.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNAKAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3918..5249)
                     /locus_tag="ACHOT6_003430"
     CDS             complement(3918..5249)
                     /locus_tag="ACHOT6_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFP9948593.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5524..6888)
                     /gene="tdcG"
                     /locus_tag="ACHOT6_003431"
     CDS             complement(5524..6888)
                     /gene="tdcG"
                     /locus_tag="ACHOT6_003431"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9948594.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMAHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6960..7349)
                     /locus_tag="ACHOT6_003432"
     CDS             complement(6960..7349)
                     /locus_tag="ACHOT6_003432"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFP9948595.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7363..9657)
                     /gene="tdcE"
                     /locus_tag="ACHOT6_003433"
     CDS             complement(7363..9657)
                     /gene="tdcE"
                     /locus_tag="ACHOT6_003433"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFP9948596.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGIRYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9691..10899)
                     /gene="tdcD"
                     /locus_tag="ACHOT6_003434"
     CDS             complement(9691..10899)
                     /gene="tdcD"
                     /locus_tag="ACHOT6_003434"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFP9948597.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10925..12256)
                     /gene="tdcC"
                     /locus_tag="ACHOT6_003435"
     CDS             complement(10925..12256)
                     /gene="tdcC"
                     /locus_tag="ACHOT6_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFP9948598.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12278..13267)
                     /gene="tdcB"
                     /locus_tag="ACHOT6_003436"
     CDS             complement(12278..13267)
                     /gene="tdcB"
                     /locus_tag="ACHOT6_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFP9948599.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13366..14304)
                     /gene="tdcA"
                     /locus_tag="ACHOT6_003437"
     CDS             complement(13366..14304)
                     /gene="tdcA"
                     /locus_tag="ACHOT6_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFP9948600.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3796 a         3888 c         3388 g         3491 t
ORIGIN      
        1 gaaagatacg aatagcattt acacaatacc cggaatagac tttcgtcact tttaattaaa
       61 gggatgtttt tatgcagtgg tatttagccg tattaaaaaa ttatgttggt ttctctggtc
      121 gcgcacgtcg taaagagtac tggatgttta ctctgattaa cgccatcgtc ggcgctatta
      181 tcaatgtcat tcaattgatt ttaggtttag agtttccatt cctttctctc atttacctgg
      241 cagcaacaat cattccggtc attgctctgt gtgttcgtcg tctgcacgat actgaccgtt
      301 caggtgcgtg ggcgcttctc tatctggtac cgatcattgg ctggttagtt ctgtttgtat
      361 ttgcctgttt agaaggcaat tctggcagca atcgttacgg aaacgatcct aagttttgtt
      421 caaattaatc ttagaattgg ggcgacattt cgcccctttt tattaacaat aacaaatatt
      481 attttccgct aaaaagtttg ggaatttccc gcagacacca ggatttcgct tcccccatac
      541 tgtcacgacg ccaggccata ataatatcga tctcgctggt cgattccggg ctgacgacac
      601 gcaaccgccc ttccgcaata tctttttcga ccatcggata cggcatcgtc gccacgccaa
      661 gccccgccag taatgcctga cgtttatctt caatcgtgct caccgttaag cgcggctgtt
      721 tgtccagcag ctgtacggtc aacaccgggc gctcacgagc ggtatccgct accgcaattc
      781 cacgatattt cacgcgcgtc acttcagata acggttccgg ctcctgatga atcgggtgat
      841 caggcgcggc aacgtagacg ttcattaagg tatagagctt gcgcgagttg atctccgacg
      901 aggaacgaaa atgcatatcc ggcgcgataa cgatatccgc ccgcccctgc tccagtcgtt
      961 cccacgcccc cgccagcact tcggtgatga ttgccagttg ggtattggct tttgccgcca
     1021 gtttgtcgat taacgggaaa aaggcaggtg tcggtaccag cgcctcggtc acaatggtga
     1081 gatgcgtttc ccaaccgcgc gcgagggctt ccgcatcggt agtcagttta tctgctgctt
     1141 ccagcaaaac gcgccctcgc tccagcaaca tcctcccgac attggtgaat ttggtgcgat
     1201 ggcccgagcg gtcaaacagc accacatcca gctcttcttc cagtttttgc atggtgtagc
     1261 taagtgcgga aggcacgcgt cccagctcat ccgccgccgc cgcaaaactg ccccggcgat
     1321 cgatcgcatc cataacccgt agtgcttcca gcgttaatgc cctttctttg gccatttcgt
     1381 tctcattcag gaaatttgaa cataccgggc agaatatctg gctaacaatg tagcgtccag
     1441 ccccttacca taaaaggaag taaagagagg tcaagaatta tgattactac ccgaactgcc
     1501 aggcagtgtg gacaagcaga ctacggatgg ttgcaggccc ggtatacttt ttcctttgga
     1561 cactacttcg acccgaaatt gttaggctat gcctccctgc gtgtgcttaa ccaggaagtg
     1621 ctggccccag gtgccgcctt tcagccgcga acctatccca aagtcgatat tttaaatgtg
     1681 attctggatg gagaagcaga gtatcgcgac agcgaaggca atcatgttca ggccagcgcc
     1741 ggtgaggcgt tgctgctctc tacccagccg ggtgtcagct acagcgaaca caatctcagc
     1801 aaagacaaac cgttaacacg aatgcagctt tggctggacg cctgcccgca gcgagagaat
     1861 ccgctgattc aaaaactggc gcttaatatg ggcaagcagc aattaatcgc ctcgccagag
     1921 ggtacgatgg gaagcctgca attacgccag caagtgtggc tgcaccatat cgtgctcgac
     1981 aaaggcgaaa gtgcgaattt ccagttgcat gggccacgcg cgtatttgca atcgattcac
     2041 gggaaatttc atgcgcttac gcatcatgaa gagaaagcgg cgctgacctg cggtgatggg
     2101 gcgtttattc gtgacgaggc taacattacg ctggttgccg attccccact gcgcgctttg
     2161 ctgatagatt tgcctgtcta gttgttttta cagggagatg atgatgagta aaaaatcggc
     2221 caaaaagcgc cagccagtga agcccgtggt ggcgaaagaa cctgctcgca ccgccaaaaa
     2281 ttttggctat gaagagatgt tgagcgagct ggaagccatc gtcgcggatg ctgaaacgcg
     2341 tttagccgag gatgaagcta ccgcgtaaag acaggttgcc ggatgcggcg aaaacgcctt
     2401 atccggccta caaaatccaa caacattaac gaattaaaca acccgtaggc cggataagat
     2461 gcgccagcat cgcatccggc atcaggtaca aaacacaaca acatcaacaa gttgagcacc
     2521 ccgtaggccg gataaggtgc gccagcatcg catccggcac aatactcaaa acctggcgtt
     2581 tatctggcct tgctcgccat aatctcgata atctgccgat ccgtttgctg catcgaatgg
     2641 cttgctaacg cacacaggtt ggcaatcgac tgctcaacat catgcgccac gatcccttca
     2701 ttgccggtca cggcggtatc atccagcgcc attaacaccg ctttccacgc agccgaagca
     2761 ctggtcgaaa ccttcatcgc gcagctgttc gacgcaccat cgcaaatcat gccgctgaca
     2821 tcgccgatca tactgctgat cgccatcgag atggtttcat aacgcccatc caccagccat
     2881 gccatcccgg cggcggcccc cattgctgcg gtcgttgcgg cacaaagtgc agacaaacgc
     2941 ggtaactggt tatggatgta aattgcgctc aaatgagaaa gcatcagcgc acgcgccagc
     3001 cgttcatcat ccgctccgaa gtgttctgct accaccacca caggcattgt tgcggtgatc
     3061 ccctggttac ccgagccgga gttactcata gccggaagcg tagcgccgcc catacgcgca
     3121 tcggatgccg cgctggtacg aatcacaatg gatgaagaga gatctttcgc cagcaaaccg
     3181 cgctcgcact gtttttccag cgtcgcgcca atatgcagcc cccacttacc gctcaaacct
     3241 tcctgcgata acgcacaatt tagcttcgcg gaatcgagaa taaagcggat cgccgcaaac
     3301 gggacttcat tgacgaactt caggatctca gccagcgtcg ttctggaaag caccgtcagc
     3361 ggagactctt gctcgccctc tgccacacac gcctgctggg taaacaccac accattgtgc
     3421 gtctcaatat gcacaatgtt ggtatgcccg ccgacgatgg tgacacacgc ccacttctca
     3481 ccgttccaga ctttggcgcg tgagaagagg atttcattgc aaggttcctg gatcttaacg
     3541 gagactttcc ccgccgccag cagtgctttg gcatcggcaa ttgcctgcgc agttgcgtct
     3601 ttcagcactt ccagcccagc tttggcattc ccacctaatg ctcccagcgc cgccgcaatc
     3661 ggcagcccca ccattcccgt gccgggaacg gtgacgccca gaccgttctt catcagattt
     3721 ggcgaaaccc aggcttctac acgttcaacc ggaccttcca gttctgccgc agcaaccgcc
     3781 gccgccagcg ccagtgaaat cggttcagta catcccagcg ccggttttac ttcctcctga
     3841 acggcgagga tgtaacgctg ccataacgga tttaaagtcg aatcaaacat attaaaaacc
     3901 ttaaaatttc aggtaaatca agaaaatgcc aggaacggag aaacacaaag caacaaacca
     3961 gtgacgataa tcaggtacag agacatccct ttgtatttgt gcaatgccgg tactttgtaa
     4021 accagccacg ccgggatcag gcaccctacc atgccgaaaa tcgggctaca gatagaggtg
     4081 aagctcaaca ccggtgcgtt cagtacgatg gcgctccagg ccagcaaaat ggcgaaaatc
     4141 atgatgccgc gctgaacaag attttcgtta atcttctcgg caggcatctt gcgacgcagg
     4201 atgttcatta cgatcccttg cgttgcttcg cgaaagccta aatagacgcc aaagaatgca
     4261 gtcatgacag caaagatatt gagaatgacg ctgaccactt tcacccacgc tgcgccgtca
     4321 ccgctaataa actgtgcggc aatcgccagc gcggaaatat tctgctcata ggcttttacc
     4381 gcttcgtcat gccccatcgc cagcgtgaac gacacggcgt agaaaaagac ggtgacaaac
     4441 aaaatgccaa acgcgatatt cattgcccgc aatgctttat gccgcgccac ttcaatagat
     4501 ttttcccgcg agcgataaga gatcaccatc gggcttaacg tctggataaa caaaatcgac
     4561 gtcagggtaa acggcagcgt aataatggcg tttttcacca gcagccccag cggcggtagc
     4621 gaaccgacgt tatacagatg ccacattccc accatcgaca cgcccagcgc cgcgaccacc
     4681 agcagcttgg tcagcaccat gccggtcgaa attttgaata acaatttctc gccgcgtgaa
     4741 gagatcgcca ccagaatgca aatcagtacc agaccataaa agggactgtc tgacagcaac
     4801 ccttccgtca cgccgaaggt atgcagatag gaagcactat cgttggtgat ggcggtggaa
     4861 tagacgaaca tccaaatcac cagcattacg aaatagagcg cacctaacag gatgccccag
     4921 tttttaccta aataaccgct aatgacactc gggtaatctt tacattctgg tgattctgcc
     4981 agcgtattaa taaacaaccg ctgaaacaga tacattgccg ggtaaccaat caccgatgag
     5041 agcaaaaata cccacaatcc catcaaaccg acctgcaccg ggagaaaaac aatccccgcg
     5101 ccaatcgcca tcccgatact cataatcacc cagccggtgt cagtactgtc gaatttgatc
     5161 gcttctcgcc actcgctctc actcattcca gcacgacccg ccggggtcga agcgtctgca
     5221 atgacgcctt tattcgatgc aatttccata atttctcgct caatattttg tagggcttat
     5281 tatttttttc cgagccgcat caaggcgata tgcggtctac gtttttgcag gcgagttatt
     5341 agaataaaga aatgatacgc gcgaggtcag agaaaatctt cacaatcaag actttgaaat
     5401 aacggagatg gataagaatt ttctactaaa ttaatcgcaa gagaatattc attctctatt
     5461 tgcggcagat cacaaaaaaa aggtgcacat ttgtgcaccc aaggatgaaa gctgacagca
     5521 atgtcagccg cagaccactt taatggccag tcctccgcgt gatgtttcgc ggtatttatc
     5581 gttcatatct ttgccggttt catacatcgt ctcgatcact ttatcgagtg aaacacgcgg
     5641 tgccgaggtg cggcgcatcg ccatccgcgc ggcgtttact gctttcacgg cattaatggc
     5701 attacgttca atgcacggga tttgtacctg tccggcaacc ggatcgcagg tcagcccaag
     5761 gttatgcgcc atcgcgattt ccgccgcatt gcatacctgc gccggactgc cgcccagtag
     5821 ttcagttaac cctgccgccg ccattgaaca ggccacgcca atctccccct gacagccgac
     5881 ttccgcgcca gagatggagg cgttcatttt atacagcgcg ccaatagccc ccgcggccag
     5941 aaaatagcgg gcaattgacc gctcgtttac cggacgacgg aacttatcgt aataagccag
     6001 tactgccgga ataatgccgc acgcaccgtt agtcggtgcc gttaccacgc gcccgccagc
     6061 tgcgttttct tcactaaccg ccagcgcgta catgttgatc cagtcgatga cattcatcgg
     6121 atcgttagag atgttatcgc tggaaaccag ctgacgacgc agcgctacgg cacggcgcgg
     6181 cacattgagc ggaccaggca gcacgccttc agtgttcatc ccacgttcaa taccgtcatg
     6241 catcacttgc cagatacggg caaaaccggc gtcaatttcc gctttgctgc gcagcgctag
     6301 ctcgttgtgc atcatcagac cagatataga caggccgttg taatcacaca ttttcagcag
     6361 ttcacctgct gagtggaaat cgtaaggtac ggacgtttcg acatcgtgcg acaggccgaa
     6421 gtgttcttct tcgacaataa acccaccgcc gacagagtaa taggttttac ttaatagcgc
     6481 ttcctgtccc ttccaggcag tgatccgcat tccgttctca tggcgaggca acatttcggg
     6541 atggaagata atgttctttg ctacaggaaa atcaacaata tgcgcaccag atgccactgg
     6601 cagccgcccg ctgcgcgtta ctaactctat aaatgcaggg atctcatcaa tgacaacatc
     6661 ctgcggactg tttcctgcca gtcccatgat gatggcgaca tccgtggcat ggcctttgcc
     6721 cgtcagtgac aacgacccgt acagatcgac cacaatatgg ctcgtcgcgg ttaataagcc
     6781 gctactttcc agccgatcaa taaaactttt tccggcattc attggcccca cggtatgcga
     6841 actggaggga ccaatcccaa ttttgaaaat atcgaatgca ctaatcatat ccacaccctc
     6901 ggattgccgt tcagtgaagt ggagcggaac gaccttacga ccgtcccgct cacgaggctt
     6961 tacgcactac gtactgcgat ggcttcaatt tccagcttca catctttcgg caaacgcgcg
     7021 acctgcacac agctccgggt cggataggtc gcctgatgct catcgaagaa ctgcttatag
     7081 acttcgttga tggtggcaaa atcattcaga tcggtgataa acacggtcat cttgatgata
     7141 tcgcccacgc tcagcccggc agcaaccacg atcgctttga cgttttcgag gcttaaacgc
     7201 gcctgatctt gcacatcagc cgggatctca ccggtctgtg ggcaaaccgg tatttgcccg
     7261 gaggtgaaga ccatgctgcc taaatcaacg ccctgaacat aagggccgat tgcgcctggg
     7321 gcacgttgcg tttcgataat ctttttcata catcctccgg cgtcagagcg cctgggtaaa
     7381 ggtacgtgaa ataacatcct gttgctgttc acgggtcagt gcgttgaagc gcacggcgta
     7441 gccagagaca cggattgtca ggttaggata tttttccggg tgctcgatgg catccagcag
     7501 catttcccga ttcattacgt tgacgttgag gtgttgaccg ccttcgacat ccgcttcgtg
     7561 gtggaaatac ccatccagca ggccgacaag gttggtttta cgtactggat cttctttgcc
     7621 cagcgccgca ggcacaatcg agaaggtgta cgagatcccg tctttagcgt aggtgaacgg
     7681 cagtttcgcc accgacgtca atgaggccac ggcacctttg cggtcacgac catgcatcgg
     7741 gttagcgccc ggcgcgaacg gtgttccggc gcgacgaccg tccggcgtgt taccggtttt
     7801 ctggccgtac accacgttag aagtgatagt cagaatcgac tgggtaggga cggcgttgcg
     7861 ataggttggc agcgctttaa ttttcttcat aaaacgttca accaggtcgc aggcaatgct
     7921 gtctacgcgc tcgtcgttgt tgccgtactg cggatattca ccgtcgattt caaagtccac
     7981 cgccaggccg ttttcgtcac ggattggttt cacgcgggca tatttgatgg cagacaggga
     8041 gtccgtcgcc accgacaggc ccgcgatgcc gcacgccatg gtgcgataga catcacgatc
     8101 gtgcagcgcc atcagcgaag cttcgtagct gtacttgtcg tgcatgtagt gaatgatatt
     8161 cagcgcgctg atgtactgca ccgccagcca gtccatgaag tgatcgaggc tgtccatcac
     8221 tttgtcgtaa tccagcacgt cgtccatcaa cggtgctgtt ttcggcccga cctgaatctt
     8281 cagcttctcg tccaccccgc cgttaattgc gtacagcagt gttttcgcca ggttagcgcg
     8341 tgcaccaaag aactgcattt gcttaccaat caccattggg ctgacgcaac aagcaattgc
     8401 gtaatcgtcg ctgttgaagt cagtacgcat cagatcgtcg ttttcatact gcaaggaaga
     8461 ggtgacgatc gacacctgcg cggcatattt tttgaaggca atcggtaatt cttccgacca
     8521 aagaatggtc aggttaggtt ccggtgccgg ccccatagtg tgcagggtgt gcaaatagcg
     8581 gaaggagttt ttggtcacca gcgtacgacc gtccagcccc atcccgccga tcacttccgt
     8641 cgcccagatt gggtcgccgg agaacagcga atcaaattcc ggcgtacgca agaagcgcac
     8701 catacggatc ttcataatga agtgatcgat cagttcctgt gcctgctgct cattgagtac
     8761 gcctgcttta aagtcgcgtt caatgtagat atcgaggaac gatgccgtgc gccccagcga
     8821 catcgcgccg ccgttctgtg atttcactgc cgccagataa gcgaagtaga gccactgcac
     8881 cgcttcctgc gcattctgcg ccgggcgaga gatatcaaag ccatatttcg ccgccatttc
     8941 ctgaatctgc aacagcgcat gacgatgctc tgccagctcc tcacgcagac ggatggtggc
     9001 ttccagatcc tcgccttttt ccagacgaga ctggagatcg gcaaattgca gttcgcgttc
     9061 acgcaccaga taacgaatgc catacagcgc tacgcggcga tagtcaccga taatgcgccc
     9121 acgtccataa ccatctggta aaccggtcag tacgccagat ttacggcagc gcagcatatc
     9181 cggtgagtaa acatcaaata cgccctggtt atgggtttta cgcagatcgg taaacagata
     9241 ttcaaattca ctgtccattt ctcggccata ggcgtggaat gaacttttaa tcatattaat
     9301 gccaccgaac gggtgtagcg cacgtttcaa cggcgcatcc gtttgcaggc caacaatttt
     9361 ttccagcggc tggttaatat atcccgcatc atgagcggta attgtggtgg caatattggt
     9421 atcgaaatca accggcgcgt gggttgcgtt ttcgatacgg atgccttcca ttactttttc
     9481 ccacaattcc gtggtggcag gcgtcgcttc ggcgaggaaa gattcatcgc cttcatacgg
     9541 tgtatagtta tgttgaataa aatcgcggac attaatttcg tttttccagt ccgtaccttt
     9601 aaagccaagc catgcgtcgg cgtacagctt atcgctggta tcaatatcta ccttcatgaa
     9661 aaataatctc tctacaatac ttcaactaaa ttatgcaaat tctgcgggcg cgttaacttt
     9721 gcctaaatga atggcatcca aagcaatcat tttttcttcg ttagtcggaa taacggcgca
     9781 aatgacatgc gcattttcac tggaaacaat tcgctcgcca aaagagttag atcgattatt
     9841 catttctgta tcaatctcta cgcctaatac agccaaatgt tccatgacca gacgacgaat
     9901 taaacttgaa ttctctccta ttccgccggt gaatataatt ccatccaggc gacgtaatga
     9961 agctgcgtgt ccggcaatat gacgggcaat tcggtgaaca aaggttttaa ttgccagttg
    10021 cgcgcgttcg tgaccttcat gccaggcttt ttccagaaca cgtaaatccg aagaaagacc
    10081 ggaaatacct aataatcccg actctttatt cactacgcgt tccaggtcac ccaggctctg
    10141 gttggtttgg ctggcgaccc aggacatcgc accaaagtcg acatcgccac tgcgggtacc
    10201 catcatcaag ccttccagcg gcgtcattcc cattgaggta tcaacactct gaccgttgcg
    10261 aaccgcgcag attgacgcgc cattgccaag atgcgccaca accaggccgg agtcatcttc
    10321 cgccagattc agcagcgaat gtgcgcgctg ggaaacatag cggtgcgacg tgccgtggaa
    10381 accataacgg cgtacaccta actcttcata atatttccac ggcaggccgt ataaataagc
    10441 ttccggagcc atcgtctggt ggaaactggt atcaaatacc gccacctgag ttacgcccgg
    10501 aaataattgc tgcgccgatt caataccact taaattggcg taattatgca ggggtgccag
    10561 tggagaaacg cgacggatat tatcaatgac ttcatcggta ataatggcgg actcggtaaa
    10621 aatactgccg ccgtgagcga tgcggtggcc aattaaggcc acactgtcat ttaaattccg
    10681 tttttccagt tcaaatgcaa ttgccttcaa tgcaccttcg tagctgtggt gagccagcgg
    10741 tgctggctct cccccattta cggataagaa tgcattttcc gagttaatac cgtcggcaat
    10801 acctgacatt aatacttcac agtcgctggc atcgagcacg gaaaacttaa tcgaagacga
    10861 accacagtta ataaccaaaa caaccggaaa ttcattcatc tcttttctca tcctgagtta
    10921 cggattaaaa cagtttgtat acgatgttca ggatggtcag cagaccaatc acggtaacaa
    10981 acacgttatc cagacgacca cggtatttcg ccagagacgg cgctttacgg atggcataca
    11041 tcggcaacag gcacagcagg gatgcgataa tcggtgcgcc catggcttca atcaggtcaa
    11101 ggatgttcgg gttggcgtag gcaacaaccc aggtggagcc catgatgaag atcatgctga
    11161 tagtgttcag tttacccagc gacactttag ttttgtcgcc tttataacca aacttcagga
    11221 ccaggccatt cagaccttcc agcgttccca gatagtgacc gaagaaagat ttgaagatag
    11281 ccacgagtgc gatgatggaa gccgcatatt ccagtgtaat cgcgaacgtt gttttggtac
    11341 cggtcatgga cgcaaagtgg ttagccagat aagaaagcac tggaatattc tgcgctttgg
    11401 cttccgccat gttggccgga gacagcgtaa acaggcagct aaaggcaaag aacatcacca
    11461 ctgcaaccat cagcatgctg gcacgagaaa tgatttggga acatttacgt tcggtgaagt
    11521 cgcgaccgaa gtctttctca tactcttcac gtttagaaac cacgaaggaa gagacgattg
    11581 gcgagaaatt aaaggagaaa accatgatgg aaatccccag ccagacagtg atcaggatac
    11641 cgtcatgacc ggttaacgac agcgaaccga ggtcaacctg gtcgataacc gcagagttcc
    11701 agtaagggat cagcgacaga gaaatcagca ccaggctggc gataaacggc cataccaggt
    11761 agctcatcac tttaaccatc agatccttac caaaccagat gacgaaagcc atcagcagca
    11821 acaggaacag cgccacaaag ccgcgattca gcggtgcaaa tccgagctgg ttttcccaga
    11881 acgtcataaa ggtattggta atagtaacgc cataaatcca cagcagtggg caaatcgcga
    11941 agaagtacag gaacgtgata accacgccgc cagttttacc aaaatgctct tccaccgttt
    12001 ccgtaatgtt gccggaaggg ttagagccag aaagacacag acgcgccagc gcccggtggc
    12061 aataaaatgc gatggggtat gccaatacca acatcagaag aatcgggatc agtccgccaa
    12121 aacctgcgcg gatagggaag aacagcaccc cggcgccgat tgccgtacca aacaagccta
    12181 acgtccatgt ggtatctgat ttacgccagg acgattgttt tgtctggctg gatacaatgc
    12241 tatctgaagt actcatatcc tatcctcaac gaattaatta agcgtcaacg aaaccggtga
    12301 tttgagagac gcgagaaaga tcgatattgc cgccggaaat aatactgacg gtttttctgt
    12361 tttgaatata ttggtctaat ttaccgctta ataatgcagc acatgccaga gcgcctgcgc
    12421 cttcggtgac gactttattg cgctgaatta aggcaatcat actgtttctg atttcgtctt
    12481 cgctgaccag cacgatgtca tcgactaatt cacgaacgat ttcgtaagtt aaattacccg
    12541 ggcgggagac atcacaacca tccgccaggg tgccggtagt tcggtgcgtg gttatttctc
    12601 cggagtggaa agaagccgcc atgccgtgaa cgttttctga ctgtacgcca ataacacgaa
    12661 tggtcgggtt aatagattta attgccaccg caataccagc aattaaacca ccaccaccaa
    12721 ttggcacaat cacgttatcg acatcataga gatcttccat aatttccaga ccaatcgttc
    12781 cctggccagc aatcactttc ggatcatcgt aaggtgggat aaaaatacga ccttccattt
    12841 cgacaatttc gctcacttta gcgatagtgt cgttgaaatt atcaccatgc agaacgactt
    12901 ctgcggagta gtcgcacgtt gccgctactt tggattttgg cgcacctttt ggcatcacca
    12961 ctttaccgtc gatacccagc atcgcgcagg agagcgaaac cccttgcgca tggttgcccg
    13021 cagaacaggc cactacacct ttacgttttt ccgcatcagt cagtgaactt aatttattaa
    13081 atgcgccacg aattttaaat gaacccgtac gctgcatatt ttcaaacttc aggaatattt
    13141 cacctttgca acgttcacta aaatagtttg agcgaggcat gcctgtttta taaattcgcc
    13201 cagccagtcg ttgtttcgct tcaataatgt catcaatagc aaccggcaga tcgtatgtaa
    13261 tatgcattat aaaacctctt cgcctgaatt aaatatgtag gtaaccgtaa ccgacacctg
    13321 caaaacagac aggtggatta tttatgttta aataaaaaca aataactaac caacttctat
    13381 taattgcctt cgtctacacc cattataaga tgaatactct ttggctaatt ccaccaaaac
    13441 cgatgctgct tttttaatac gataattttt cgaccatacc gcggcatatt gtgccacagg
    13501 taatgtttct tcaaccggaa tagtaataaa ttgatttgaa ccaaaaggtg acgtcatatc
    13561 acaaggaatt acagttaaga aatcagcatt gagaacaaga ttataaattg tcacgactga
    13621 gtcagtttta acgatgtttt caatactgat gccatttctt tgtaacgtag taagaagctc
    13681 gctgtagtac cccatattgg tttgtggcaa cacccactgt tcgttcttca acgactccag
    13741 cgtggtggtg ccggtgcatg ttcgggactt actggctacc agcacaaact cggactcgaa
    13801 cagcggctca acatgtaaat cctgaagctt catttctgca cttaacgtac caatcgcaaa
    13861 atccagtcga ccgtcgcgga ttgccggtaa gaacgaagac agttgcgctt catacataga
    13921 aacctgcgct ttcgggaaca cctctttgaa cttgttgatc atccctgaca taaaagtaaa
    13981 accaatcaat gaaggaaaac caaatgagac ttccaccacc gcctcagaag acataccgct
    14041 tatctcatta accatatttt tcatttcacg ggtaatggat tcggaacggg agagtaacaa
    14101 ttgaccggca ggtgttaatg ttacaccggt atttttccgc accactaatt ccacaccaaa
    14161 ataatcttca atatcgttaa tgattttact gacggctggt tgagttaacc ctaattcttt
    14221 tgcagccgag ccgatagagc cacttctaat gacttcctga aagactacca ggtgctgcgt
    14281 tttcggaaga agaatagtgc tcataacgac ctacgttaat tacctcattg acggcatgaa
    14341 gtgtatcaaa atgaaatgaa caggatatgt gcgaccactc acaaattaac tttcaatact
    14401 ttccagggta tcgttattac aattaaatac ctttaaatat caacaagtta aagcataaaa
    14461 atcagcataa accctgattt ttgtcaaaaa aaatgacggg gataaccata ttttatggcg
    14521 ataacatcat tcgttatgtg gttaatacaa aaaaggggct gag
//
LOCUS       DAZKIV010000116        14416 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZKIV010000116 DAZKIV010000000
VERSION     DAZKIV010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.116"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(126..794)
                     /gene="artM"
                     /locus_tag="ACHOT6_003438"
     CDS             complement(126..794)
                     /gene="artM"
                     /locus_tag="ACHOT6_003438"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFP9948601.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(794..1510)
                     /gene="artQ"
                     /locus_tag="ACHOT6_003439"
     CDS             complement(794..1510)
                     /gene="artQ"
                     /locus_tag="ACHOT6_003439"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFP9948602.1"
                     /translation="MNEFFPLASASGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1517..2248)
                     /gene="artI"
                     /locus_tag="ACHOT6_003440"
     CDS             complement(1517..2248)
                     /gene="artI"
                     /locus_tag="ACHOT6_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFP9948603.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2266..2994)
                     /gene="artP"
                     /locus_tag="ACHOT6_003441"
     CDS             complement(2266..2994)
                     /gene="artP"
                     /locus_tag="ACHOT6_003441"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFP9948604.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3212..3727)
                     /gene="ybjP"
                     /locus_tag="ACHOT6_003442"
     CDS             complement(3212..3727)
                     /gene="ybjP"
                     /locus_tag="ACHOT6_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFP9948605.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3853..4176
                     /gene="ybjQ"
                     /locus_tag="ACHOT6_003443"
     CDS             3853..4176
                     /gene="ybjQ"
                     /locus_tag="ACHOT6_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFP9948606.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4173..5003
                     /gene="amiD"
                     /locus_tag="ACHOT6_003444"
     CDS             4173..5003
                     /gene="amiD"
                     /locus_tag="ACHOT6_003444"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFP9948607.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(5000..6013)
                     /gene="ybjS"
                     /locus_tag="ACHOT6_003445"
     CDS             complement(5000..6013)
                     /gene="ybjS"
                     /locus_tag="ACHOT6_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9948608.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6112..7542)
                     /gene="ybjT"
                     /locus_tag="ACHOT6_003446"
     CDS             complement(6112..7542)
                     /gene="ybjT"
                     /locus_tag="ACHOT6_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFP9948609.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7553..8554)
                     /gene="ltaE"
                     /locus_tag="ACHOT6_003447"
     CDS             complement(7553..8554)
                     /gene="ltaE"
                     /locus_tag="ACHOT6_003447"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFP9948610.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGTDVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8591..10309)
                     /gene="poxB"
                     /locus_tag="ACHOT6_003448"
     CDS             complement(8591..10309)
                     /gene="poxB"
                     /locus_tag="ACHOT6_003448"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFP9948611.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10442..11410)
                     /gene="hcr"
                     /locus_tag="ACHOT6_003449"
     CDS             complement(10442..11410)
                     /gene="hcr"
                     /locus_tag="ACHOT6_003449"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFP9948612.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVQVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLTRVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLFLA"
     gene            complement(11422..13074)
                     /gene="hcp"
                     /locus_tag="ACHOT6_003450"
                     /gene_synonym="priS"
     CDS             complement(11422..13074)
                     /gene="hcp"
                     /locus_tag="ACHOT6_003450"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFP9948613.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMANLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13218..14117)
                     /gene="lysO"
                     /locus_tag="ACHOT6_003451"
     CDS             complement(13218..14117)
                     /gene="lysO"
                     /locus_tag="ACHOT6_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFP9948614.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3482 a         3850 c         3819 g         3265 t
ORIGIN      
        1 tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac cgtgctgatg
       61 tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga atgtacgcag
      121 ctacattaat ttcgccgttc gaatgccagt gctttgcgct cgatcagacg catcatcagc
      181 gtcagcaggc cgttgacgac caggtaaata atccctgccg caccgaacac cattacatcg
      241 taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt aatggtgtaa
      301 gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga gagcgagcgt
      361 ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc cagggcgcta
      421 caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag ctgcgtggta
      481 tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc tgacaacaaa
      541 tgccacagtg ccggatactc ctgcaaagtc ggaaactggc ccggcccgta ataaatcagg
      601 aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg caccagccac
      661 accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag aatcagtgcc
      721 acaatcagcg aggcaacggt tagcgtcagg ctggtgtgta gccctttcat cagttcgggt
      781 aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg tcaatgcgtt
      841 tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc acaatgtacc
      901 aggtaaatgg ttcctgggta cgagtagcga tgctttttgt ttgcagcatt aaatcattca
      961 cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg ttaccgaggc
     1021 caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata gccgatttcg
     1081 acagccccag cgcctgacca gactcccact gacccaccgg caccgctttc aacgcgcccc
     1141 gaagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca ccacaaagga
     1201 acgggctaac gtcgaagttc tcaatgtcca tctgcactgg gatctgcacg aacccaagat
     1261 tgatagtgaa gccatccgaa agcgtcagca gcagctgcga ggagccaaaa tagataaaca
     1321 gcaccaccag aatttctggc aggccacgca gaatggttac cagcgctgaa cctgcccacg
     1381 cgacaggacg ccattttgcc gactcccata ccgcaaagaa catcgccagc gccagcccga
     1441 caatcaatgc acaaacggca aggccgacgg tcatcccgga ggcgcttgct aaaggaaaaa
     1501 attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg taagtgccat
     1561 ctttcttcac tttttccagc gcagtgttga gtttctgctg cagctcagtg ttgccctgac
     1621 gtaccgcgat gccgaggcca gtgccgaagt aatctttatc ggtcactttg tcgcccaccg
     1681 ccgccagttt cgggttatct ttcagccact cagtgaccac tgcggtgtca ccgaagacgc
     1741 cgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca tacggaacgg
     1801 tagtgatttc cgggtgctta tccataatga atttctggtg tgtcgtcccg ttctgtacgc
     1861 cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga cccacaaaca
     1921 gggcagagtt gtcatagtac ggggtggtaa acagcacctg cttttcacgc tccggagtga
     1981 tatccatgcc cgccatcacg gcttctacgc gacggaattt caggcttggg atcaggctgt
     2041 caaacgcctg gttagagaaa gtgcaggttg catcaatctc tttacacagc gcttgtgcca
     2101 ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac ggaggatagg
     2161 atgcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta aaacctgcaa
     2221 ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt gagagagata
     2281 gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt gttctacgat
     2341 atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt caacttcgtg
     2401 ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc tgacgatttg
     2461 tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta cctgcggttc
     2521 catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa gatgcagcgg
     2581 gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg cacgggccag
     2641 cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt tttgctgcac
     2701 ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac gacgcaaatc
     2761 gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg caatgttgag
     2821 cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc ttttacccgc
     2881 gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg tgatatcgaa
     2941 cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac tcattgacac
     3001 tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttga cagaataatt
     3061 atgcaatatt tctgctttaa aagttaaaag caaagcgcat tattcaataa acatagcaca
     3121 aaataacggg ggcggtggtc ggcgagcata aatgtcggca ttcctcacga aatgccggac
     3181 aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac tgacggagcg
     3241 tacccgccgt ggcatgaacg ctgccgccta agtagcgtac atcgtcgatc acccaacact
     3301 ggccttcctg aatcattaac acttcatcct gccagctctg atcaccctgt tttagatcca
     3361 cacgcagagg aatatttcgc gcatcacgat tcgggattgt tgaggcactg gcaacacggg
     3421 cgctctctgg caaggttgtg cgactggaga atggatcgtt ggtcagtagt tcccgatggt
     3481 tattatcccg ggaggcatcg ctaagcagtg tcgccagttt gtcgctcaga taagggcgca
     3541 aggcggtgat gtcgttgctg cggtgcaaaa tgcggtagtc ataaaattgc tgggccacgt
     3601 tatccgggcc tccttcaacg caaggaccac tgcgtgtgcc gttatcttta taagctggag
     3661 tgactgtggt gcaggcactg aggagcagtg cgcaggggat aagcattgtc aatttgctgt
     3721 agcgcataat gatttcctta taagcgatcg ctctgaaagc gttctacgat aataatgata
     3781 tcctttcaat aatagcgtat cagtctgata atgctttaga gatcgaaggc ttaacaaaca
     3841 aggagatcga tcatgcaatt ttctacaacc ccaactctgg aaggccagac catcgttgaa
     3901 tattgcggtg tggtgaccgg cgaagcgatt ttaggtgcca atattttccg tgatttcttt
     3961 gccggtatcc gcgatatcgt tggcggacgt tccggtgcgt atgaaaaaga actgcgtaaa
     4021 gcacgggaga tcgcctttga ggaattaggc tcccaggcgc gggcgctggg ggccgatgcc
     4081 gtcgtcggta ttgatatcga ctacgaaacg gtcgggcaaa acggcagtat gctgatggtt
     4141 agcgtcagcg gtacggcggt gaaaacgcgt cgatgagaag agtcttctgg ctgatcgctg
     4201 ccgctctgtt actggcaggg tgtgcaggcg aaaaaggcat tgtcgagaaa gagggatatc
     4261 agcttgatac ccgacgccag gcgcaggcgg catatccgcg cattaaagtg ctggtgatcc
     4321 actacaccgc agatgatttt gatagctcgc tggcgacact gaccgataag caggtcagct
     4381 cgcattatct ggtccctgcg gtaccaccgc gatacaacgg taaaccgcgc atctggcaac
     4441 tggtgccgga acaagaactg gcctggcatg cggggattag cgcctggcgc ggggcaacgc
     4501 gccttaacga cacctctatt ggcattgagc tggaaaaccg tggctggcaa aaatcggccg
     4561 gagtgaaata ttttgccccg tttgaaccgg cacagattca ggcgcttatt ccactggcga
     4621 aagatattat tgcccgttat cacatcaagc cggaaaacgt agtggcacat gcggatatcg
     4681 caccgcagcg caaagacgat ccggggccat tatttccctg gcagcaactg gcgcagcagg
     4741 ggattggtgc ctggccggat gcgcagcggg ttaactttta ccttgccggg cgcgcgccgc
     4801 acacaccggt agataccgcg tcattgctgg agcttttggc gcgctacggt tatgacgtta
     4861 aacctgatat gacaccgcgc gagcagcggc gcgtgattat ggcattccag atgcatttcc
     4921 gcccgacgtt atataacggc gaagcggatg cagaaactca ggcgattgcc gaagcattgc
     4981 tggagaaata cgggcaggat tagcgcggca gttttccgtg gtcgcgtagc caggcggcag
     5041 ttttctcgat accttcatcc agggtgatga ccggctgata acctaactct tcctgcgcac
     5101 gcgtaatatc cagcgtaaag tcaaaattca acttggagac gccgtagtgg gtcagcggcg
     5161 gctcttttgc tgacttgcgg cctaaacgct ccatgctgcg ggcgatcata tccagcatcg
     5221 ggtaggggac ggaacgaata cgacagtcaa tattcaactc gtcgatcagc ttctgcacga
     5281 tgctgcgcag tgtgcgatgc tcgccgttgg tgatgttgta cacacgcccg gaaggtagct
     5341 tatcgcaggc ttcctggctt gccagccaca ttgcgtgcac ggcattttca tagtaggtca
     5401 tatcgaccag cgcactgccg ccatgcggta acagaatact gccgtagtgg tgcatcatat
     5461 gcgccagacg gggaataaag actttatcgt gcggtccgaa cagactttgt gggcgcagaa
     5521 tagtaaagcg cgtttgtgga ttcgcctgcg aaagcatatt gatcacttct tcgctggctg
     5581 ctttgctgcg ggcaaactcg ttggcgaagc ggtgagggcg aaaatcttct ttaatatcgc
     5641 gatggtggtg ataatcgaag tacagggagg gggaagagat atgaataaag ttacgcacac
     5701 cccaggcgac agcccattca cccaggcggc gagtggcgcg aacgttagcc agatcgaaag
     5761 cctgttgtgt cccccagggt gaggtaaagc tggagcagtg ccacagcgta tcaatgcccg
     5821 cgagcatcac tttagcttgt gatgaaacca gctcggtcag atccgccgga acaaactctg
     5881 cgcccatttt ttccagcaat ttgcccattg cctcgttgcg accggtcgct cgcacgctga
     5941 tgcctttctg gcataaaaac tctaccgcgt ttcgacctaa gccgctggtg gcgccggtaa
     6001 ccagtacctt catatcaatc cactgttgtt gagaaaataa cgtgcgcatt cttccgtgat
     6061 ttcccccatg atgcaatggg aaacatgaaa gaataacgca ggttttgtcg attaatctgt
     6121 gctttgttct gccagtctgg cgatttgttt tgccattccg cgaaaaataa acagatgcgc
     6181 ggggatcatc aataaccagt aaaacagccc cggcataccg tgcggatgcc agaaagcgcg
     6241 gacatcgata gtacgatagt cgcctttatc ttccaggcta aaacacagtc gtcccagccc
     6301 cggcgctttc atgccaaata acaacgtaag ttgtttttcc ggttcaacga caatcacttt
     6361 ccagctatct accgcatcgc cagtctgtaa atattcgcgc tccgggcggc ctttcgccag
     6421 cttatgaccg atcgcgcggt ccatcaacgc ccgtgtctgc cacaaaatat tgccaaagaa
     6481 ataacgctct ttaccgccga tttggttcac tacctgccat aaagcagcaa ggctggcgga
     6541 cgttttaacg gtaaaccccg cctgtttggc aaaataaccg tactccggtc gccagcgggc
     6601 aaaggcctga gcgtcgtagc cccagtcgct ggagttgacc agtttttcct cctctttcaa
     6661 cgtgctacgt accgcgtcat cgaaagcgat cagccgttgt gggatgagtg cacgtagcgc
     6721 ggtatcatcc gccagcagat cgtgtttcag cccctgaatc aacgccctgg cggtggtggg
     6781 cggtacggaa gtaatcacat tgagaaacca caccgaaatc cagcgggtgg ggagggggat
     6841 ggggatcaac cagcggcgct taccgctcac cgccataaaa tgttcaaact gttgctgata
     6901 actgagcacc tctggtccgg cggcttcgaa gatgcggtgt tcgctggccg gatgatctaa
     6961 caacgccacc agatagtgca gcaagttttc cagcgcgatg ggcgtggtgc gtgaacgtac
     7021 ccagcgtggc ggcgttaaca ctggcaggtt gtagaccata tcgcgcatga cttcgaacgc
     7081 cgctgaacct gcgccaacga taattccggc ccgaagttcg gtcacaggta cattcgcttc
     7141 acgaagaatg tccgccgtag cctgacgagc acgcagatga tccgactgct catgtggcgg
     7201 ggcctgcaac gaactgagaa agattaattg cttaactggt acttcacgta gcgcatcgcg
     7261 gacgttgaga gccacctggc gctcctgagc gataaaatcg ccgccttcgc ccatgctgtg
     7321 caccagaaaa tagacggtat cgatatcctg caacagggcc ggaaggttat ccggccagct
     7381 gagatcgact ttatggcaac tgacgtttgc cagttgcagc tttgcaagcc tgtcgacatg
     7441 acgtgccgcc gccaggatct gatgcccttg ctggctgagt gtgcgcacca gatgctgacc
     7501 aatgtagcca ctggcaccga gaactaaaat gcgttgcggc acgtctctct ccttaacgcg
     7561 ccaggaatgc gcgccagtgg gcggcgactt ccgccagttg ttcgcgcgag acgtcaagat
     7621 gcgtcaccag gcggacaatc ggcgaggcgt taatcagcac gtttctcgct ttcatgtatt
     7681 cgcctaacgc ggcagcattt tcttccccga cgcgaacaaa cagcatatta gtgtcctgac
     7741 gcatcacatc cgtgcctgct tcacgcagtt gctccgccat ccaggcggcg ttgtcgtgat
     7801 cttcctgcaa ccgtgcgacg ttatttttca gggcatacat cccggcggca gccagaatcc
     7861 cggactgacg catcccgcca ccggccattt tccgccagcg gatggcacgt ttaatgtaat
     7921 cacgattacc gacgagtaat gaaccgactg gcgtcccaag acctttcgac aggcaaatgg
     7981 tgaacgaatc acaatattgc gtgatctctt tcagttcgca gccgtaagcc accacggcat
     8041 taaagatgcg cgcaccgtca acatgcagcg ccagattgcg ctcgcgggta aattcccatg
     8101 cttctttcag gtattcacgc ggcagcactt tgccgttgtg ggtgttttcc agactgagta
     8161 atttggtgcg ggcgaaatgg atatcgtcgg gtttgatttt catcgccact ttatccagcg
     8221 gtagcgtgcc gtcggcagcc gcgtcgatgg gttgcggctg aatactgccc agcaccgctg
     8281 cgccaccggc ttcaaacaga tagttatgcg cggcctgacc gacaatatac tcttcgccac
     8341 gttcgcagtg actgagcaga gcgaccaggt tggcctgagt gccggtaggc agaaaaatgg
     8401 cggcttcttt accggaaagc tctgctgcat agtcctgcag agcattaacg gtagggtcgt
     8461 ctccgtaaac gtcgtcccca accggggcgg ccatcatcgc ttcgagcatg gcgcggctcg
     8521 gtcgggtaac ggtatcactg cgtaaatcaa tcatggcatg tccttattat gacgggaaat
     8581 gccacccttt ttaccttagc cagttcgttt tcgccagttc gatcacttca tcgccgcgtc
     8641 cgctgatgat tgcgcgcaac atatacaagc taaatccttt ggcctgttcg agtttgatct
     8701 gcggtggaat ggctaactct tctttggcga ctaccacatc caccagcacc ggaccgtcga
     8761 tggagaaggc gcgttgcagg gcttcatcaa cttcagacgc tttttctaca cggatacccg
     8821 taatgccgca cgcttcggca atgcgggcaa agtttgtgtc gtgtagttcg gtgccgtcag
     8881 tcaaatagcc accagctttc atctccatcg ccacaaagcc cagcacgctg ttgttaaaga
     8941 cgacaatttt cactggcagt ttcatctgca ctactgagag gaaatcgccc atcaacatgc
     9001 taaaaccgcc atcgccgcac atggcgacca cctgacgttc tggctctgtc gcctgcgcac
     9061 ccagcgcctg cggcatggcg ttagccatcg aaccgtggtt aaacgaacct aacaggcgac
     9121 gcttgccgtt catttttaga taacgtgccg cccacaccgt tggcgtacca acgtcacagg
     9181 tgaaaatagc gtcatcggcg gcaaaatgac taatttgctg cgccagatat tgcgggtgaa
     9241 tggctttctc gctcggttta gctaaatcgt ccagcccttt gcgggcgtcg cggtaatctt
     9301 ccagcgcttt atccagaaac ttgcgatcgg ctttttcttc caccaatgga agcaatgcac
     9361 gcagagtcga cttgatatcg ccgaccagtg ccatatccac cttgctgtga gcgccgatgc
     9421 tggctgggtt gatatcaatc tgaatgattt tggcatcggt cgggtagaag gcgcggtagg
     9481 gaaattgcgt gccgagtagc actaacgtgt cggcgttcat catggtatgg aaacctgacg
     9541 agaagccgat taacccggtc attccaacat catacggatt atcgtattcg acatgttctt
     9601 taccgcgcag ggcatgaaca ataggcgctt taattttccc ggcaaactca actaactctt
     9661 tatgcgcccc cgcgcagccg ctgccacaca tcagggcgat attgctggaa taacgcagca
     9721 gttgcgccag tttgcgtaac tcttcttctt ccggcgtcac gactggttgt ggcgcatgat
     9781 accagtgcgt ggttgcccct tctggcgcag gttttaacgc cacgtcgcct ggtaacacga
     9841 caaccgaaac gccacggtta agcaccgctt tgcgcatggc aatcgccagt acttgtggga
     9901 tctgctccgg gctggaaacc agctcgcaat agtgactaca ttcgcggaat agctcttgtg
     9961 ggtgggtttc ctggaaatag ccgctgccaa tttcgctgga gggaatatga gcggcaatcg
    10021 ccagtaccgg aacgtgattg cggtggcaat cgaacaggcc gttgattaag tgcaggttgc
    10081 cggggccgca cgatccggcg cagaccgcca gttctccgct aagttgtgct tcagcgccag
    10141 cggcaaaggc cgccacttct tcgtggcggg tggacatcca ctcgatggtg cccatgcgat
    10201 taagactgtc actaagaccg ttcagagaat cgcctgtgac tccccagatg cgtttcacgc
    10261 ctgccgattc gagtgttttg gcgatataag ctgcaaccgt ttgtttcatg gttctccatc
    10321 tcctgaatgt gataacggta acaagtttag ttcatctgac ggagggggaa gggatgggag
    10381 agaaaggagg cactaacggt taaatagccc gatgaaagga atatcatcgg gcataaggcg
    10441 attatgcgag aaacaaatcc ccctgcggat ggcaggagca ggccagtacg taaccttcag
    10501 cgatttcggc gtcggtcagc gtcattgtgc tgctcaccgt atattcaccg gaaaccactt
    10561 ttgtcttaca gcagccgcaa acacccgcac ggcaggcggc gacaaccgga acgttattgc
    10621 tttccagcgc ctccagtagc gtggtgccaa ccggggcgta aaattctcgt gccggttgca
    10681 gtttggtgaa tttcagaccg ctggtcgctg cttccgctac tggggtgaag aatttctctt
    10741 taaagaaacg cgtcacgccg agcgctttca cttcctgctc tacccaatcc atatacggag
    10801 ccgggccgca ggtcatcacg gtacgtgaag ctaagtcagg aacgcgagtc agcagttcgc
    10861 gagtgagacg accagcgata aagccttcgg taacgttatt ttccgccacc agcgttaccg
    10921 gatagttacg ccactcatcg gcgaaaataa catcctgcgg cgtgcgcacg ttgtagatca
    10981 cctgtacatc ggcctgtgga cggttcttcg caagccagcg acgcatcgac ataatcggcg
    11041 tgacgccgca gcctgccgcc agcaacagga atttatcttc tgctttatcg tcgcaggtaa
    11101 attcccccat cgcgtccgaa agccagagat aatcaccgcg ttttacatcg cgcgtcagcc
    11161 actgggagcc gacaccgtca tcaatccgcc gcacggtcag ggtgatatat tcactcacgc
    11221 ctggcgtgga ggaaatggtg taagcacgca gcgtttccgc tgagtttcgc acgctgacca
    11281 gtgcatattg cccggcgcga tatgggtagt aatcgtggca aatcagggaa atcgtccaca
    11341 catccggcgt ttcttgcgta atgtgatgaa cctgcatccg ccacgggcat tgattcgttg
    11401 gcatcgtcat cgacaaactc cttacgcgct caacagttgc ttcatgtctt cttcaacagt
    11461 ggtgatagaa cgcaggccaa atttctcgtt cagcaccgcc agcaggtctg gtgtcaggaa
    11521 accaggtgca gtcgggccgg tgacgatatt tttcacgccc agagaaagca gcgtcagcag
    11581 aatgacgatc gctttctgtt caaaccagga gagcaccagc gacagcggca gatcgttgac
    11641 accgcagccc agtttctctg ccagagtgac agccagaata atcgctgagt aagcatcgtt
    11701 acattgacct gcatctacca gacgcggcag accttcgata tcgccaaact caagtttgtt
    11761 aaagcgatat ttaccacagg cgagggtcag gatcaggcag tcatccggca cgctggtggc
    11821 gaaatcggtg aagtagtggc gctcgccgcg tgcgccgtca cagccaccaa gcaggaagat
    11881 atgacgcagt ttttcacggc tcaccagatc aatcagcgta tcagcagcgc caagcagcgt
    11941 ctggcgacca aaacccacgg tgataaggtg cggaatttcg ctgtacggga agcctgccat
    12001 ctgttgcgcc tgggtgataa ccgcagagaa atcatcaccg tccagatgac gcacgccagg
    12061 ccagccaaca atgctgcggg tccagatacg atcgtcataa gcgcctacgg ttgggtcgat
    12121 gatgcagttc gaggtcatca cgatggggcc agggaaacga gcgaactcca cttgctgatt
    12181 ctgccagcca ctgccgtagt taccgaccag atgcttgaat ttacgcagct ccgggtagcc
    12241 atgcgcaggc agcatttcgc cgtgggtgta gacattaacg cccgtgcctt cggtctgttc
    12301 cagcaggttg tagagatctt tgagatcgtg accggaaatc agaatgcatt tacccgccgt
    12361 cgctttgacg ttgacctggg ttggcgtcgg gtgaccgtat ttaccggttt cgcctgcatc
    12421 cagaatgctc atcactttga agttcatctg gccgatttcc attgaacact caagaagcgc
    12481 gttcatatcg gcaggccagg tccccagcca cgccatgatt ttatgatact gggcataaat
    12541 atcgttgtcg tattgaccga gaacatgcgc gtgttccata taggccgccg cacctttcag
    12601 gccatacagg cacagcagac gcaggccgag aatgttttcg ccaatcgccg ctttatcttt
    12661 gttaggggta aattctgctg cctgacgttg cagctcgccg agatcatcgc tcaccagttg
    12721 caggttagcc atcgggttat cgacgcgcgc gttggcatct acagccaggc attgtgcttt
    12781 cagcgcctcg cgcaaggcaa tcgcttcacg agcgtagccg acaatacgcg gagaatcgaa
    12841 gttaacgttg gtcagggttg agaaaaaggc acgtggcgcg aagctgtcaa catcgtggtt
    12901 gatgatgccg tattcacgcg cttttaccgc ccaggcagaa agcccttgca gcgccgcgat
    12961 gagtaaatcc tgaaggtcag aagtttccgc cgttttacca cacatcccct gcgcgtatga
    13021 gcagccgttt cctgccggag tacggatagt ttgttcacat tgcacacaaa acatgatcac
    13081 accttttaaa gttatattta atatacatgt ttaaggttaa gacgcttaac gcggggataa
    13141 aagggatttt tcatgcaact ttaagggaga ttgatttagc gcaattttgg cggcagggat
    13201 ctaccgccag agaggtatta cgcagagaaa aaagcgatga ggatcggcac taacaggcta
    13261 agaataaaac cgtgaacaat tgccgccggg accatatcca gcccgccagt acgttgaaga
    13321 acgggcaggg tgaaatccat tgatgtggca ccgcataagc ccagtgcagt agagcggctg
    13381 cggcgaatca gcccagggat caacataata gcaatcagtt cacgggccag atcattaaaa
    13441 aacgccgcgc tcccgattac cggaccaaaa gattcggtca ataaaatacc ggaaagagaa
    13501 taccagccga aaccggaggc cattgccagc gcggtattga tggggagatc aagaataaag
    13561 gcgttaatta aaccaccaat taatgaactg acaaccacca ccacggcgac aatcattccc
    13621 cggcgattaa ggacaatctg ctttaaggtc atgccattat tgcgcaactg aataccaacg
    13681 aggaaaagta gcaaaattaa cgtgtattca ctggcttcgg tcgcgtgttg taagaaagcc
    13741 agtccactca gaccaatggc aaaaccaatc actactacgc cgcacagttt tagcgactcc
    13801 agcgccatcg caatacgcga cgggagtttt tcttgctgat ggtggttgcg ccacggcagg
    13861 cctcgctcca gccacatcag ggcggcaata ttacacagta aaataacggt aatactgacg
    13921 gcagaataat gcagaatcgc caacaggtta ctggcgaggt tatcgagaaa cgccagactg
    13981 atacccataa aaaagagaat aaggtaaacc atccagctta atagctgatt aataactttt
    14041 aacgcagctt gttggcgaag cggaatgagg taacccacaa tcaggggaac cagaatgatt
    14101 aacagcccag aaaacatgaa aacccagccc ttgcaaagat gaagtcgaaa tgcgcgatga
    14161 cacactactg aaagcggaag gacgagtaaa gttgcaatta aaaagaaatg ttatgcataa
    14221 ggatcggtag ggtattcatt ttcatttaaa gatattcttg cgctttaatt acaaaccgca
    14281 ccggtgttgg tggcgtcaaa atcgccgagg cgttccctga aggccggggc agcccacatg
    14341 gatgcgggct gagggcgcgt tttacaggga tgttacctcg cgcccgaccc ggtagccgta
    14401 agggataagt cgaggg
//
LOCUS       DAZKIV010000117        14411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZKIV010000117 DAZKIV010000000
VERSION     DAZKIV010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.117"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            94..636
                     /gene="zapC"
                     /locus_tag="ACHOT6_003452"
     CDS             94..636
                     /gene="zapC"
                     /locus_tag="ACHOT6_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFP9948615.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(633..1742)
                     /gene="ycbX"
                     /locus_tag="ACHOT6_003453"
     CDS             complement(633..1742)
                     /gene="ycbX"
                     /locus_tag="ACHOT6_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFP9948616.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            1986..4094
                     /gene="rlmKL"
                     /locus_tag="ACHOT6_003454"
     CDS             1986..4094
                     /gene="rlmKL"
                     /locus_tag="ACHOT6_003454"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFP9948617.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4106..6013
                     /locus_tag="ACHOT6_003455"
     CDS             4106..6013
                     /locus_tag="ACHOT6_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9948618.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6143..7396
                     /gene="pqiA"
                     /locus_tag="ACHOT6_003456"
     CDS             6143..7396
                     /gene="pqiA"
                     /locus_tag="ACHOT6_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFP9948619.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7401..9041
                     /gene="pqiB"
                     /locus_tag="ACHOT6_003457"
     CDS             7401..9041
                     /gene="pqiB"
                     /locus_tag="ACHOT6_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFP9948620.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9038..9601
                     /gene="pqiC"
                     /locus_tag="ACHOT6_003458"
     CDS             9038..9601
                     /gene="pqiC"
                     /locus_tag="ACHOT6_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFP9948621.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9857..10024
                     /gene="rmf"
                     /locus_tag="ACHOT6_003459"
     CDS             9857..10024
                     /gene="rmf"
                     /locus_tag="ACHOT6_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFP9948622.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10094..10612)
                     /gene="fabA"
                     /locus_tag="ACHOT6_003460"
     CDS             complement(10094..10612)
                     /gene="fabA"
                     /locus_tag="ACHOT6_003460"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFP9948623.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10681..12441)
                     /locus_tag="ACHOT6_003461"
     CDS             complement(10681..12441)
                     /locus_tag="ACHOT6_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9948624.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANATRTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHTKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12627..13079
                     /gene="matP"
                     /locus_tag="ACHOT6_003462"
     CDS             12627..13079
                     /gene="matP"
                     /locus_tag="ACHOT6_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFP9948625.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13155..14195)
                     /gene="ompA"
                     /locus_tag="ACHOT6_003463"
     CDS             complement(13155..14195)
                     /gene="ompA"
                     /locus_tag="ACHOT6_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFP9948626.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
BASE COUNT         3578 a         3500 c         3868 g         3465 t
ORIGIN      
        1 tatttagaca ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg
       61 ttgctgcggt tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg
      121 cgttggtatt acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta
      181 tttcgctcac gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt
      241 tgcgttgacg acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta
      301 tctaaagaac aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa
      361 ccgcaaatgc cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg
      421 ggcgatgcgg cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt
      481 gaatctggcg aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt
      541 cgcgcgatgc agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt
      601 aatgttgaca gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc
      661 ggtacacagc tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc
      721 gtgacttcgc cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa
      781 taagggatac gaattccctg attttctaat tgctccagca acacctgttg gttatttcca
      841 cgaaatgcct gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg
      901 gcggtatcat cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc
      961 acctcatcgc caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg
     1021 acatcgccgt tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc
     1081 ggatgttttt gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta
     1141 accacatcaa acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct
     1201 gacgcgccgg aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa
     1261 cgttgttgga gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca
     1321 aatgacagag gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag
     1381 cgtaattgca cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca
     1441 attcgcgcgg taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca
     1501 gcaaaacgaa catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca
     1561 ggtgagggag taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca
     1621 ggttccgtga tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt
     1681 gtaagaccaa tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc
     1741 acggtgcggt cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc
     1801 tataatgcgc aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg
     1861 gccctgggtt gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt
     1921 ccatgtccag actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg
     1981 acgatatgaa ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg
     2041 aactggaaaa cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg
     2101 gcgacacacg gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt
     2161 tgccgctggg cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga
     2221 tcaactggac agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga
     2281 atgacaccat ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg
     2341 ctttcacgcg gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg
     2401 ttaacgtctg gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt
     2461 tacatctgcg tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag
     2521 ccgctattgt gatgcgatcc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg
     2581 gttccggtac gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc
     2641 accgtgggcg ttggggcttt agcggctggg tgcagcatga tgaagatatc tggcaggaag
     2701 tgaaagcgga agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt
     2761 acggttcgga cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg
     2821 ggattggtga actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc
     2881 cgaaagggcc gtacggtaca gtgttgagca acccgccata cggggaacgt ctggacagcg
     2941 aaccggcgct gattgcgctg catagtctgc tgggccgaat catgaaaaac cagttcggtg
     3001 gctggaatct ctctttgttt agcgcctcgc cggatctgct cagctgcctg cagctgcgtg
     3061 cagacaaaca gtacaaggcg aaaaacggcc cgctggactg cgtgcagaaa aactaccacg
     3121 ttgccgaaag cactccagac agcaaaccgg caatggtggc ggaagactac gccaaccgcc
     3181 tgcgtaagaa cctcaaaaaa ttcgagaagt gggctcgtca ggaagggatt gaatgttacc
     3241 gcctgtatga cgccgatctg ccggaatata acgttgccgt tgaccgttat gccgactggg
     3301 tggtggtgca ggagtatgcg ccgccaaaaa ctattgatgc gcacaaagcg cgtcagcgtc
     3361 tgttcgatat tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtac
     3421 tgaaaacccg tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg
     3481 agtttctgga agttaccgaa tataacgctc acttgtgggt gaatctgacg gattacctcg
     3541 ataccggtct gttcctcgat caccgtatcg cccgtcgtat gctcggccag atgagcaaag
     3601 gcaaagattt cctcaacctg ttctcttata ccggcagcgc caccgtgcat gcgggattag
     3661 gcggtgcacg cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac
     3721 gcaacctgcg tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc
     3781 tggcgtggct gcgtgaggca aatgaacagt tcgatctgat ctttatcgat ccgccaacct
     3841 tctctaactc aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat ctggcgctga
     3901 tgaaagattt gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac
     3961 gtggcttccg tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta
     4021 cgcaaaaaac gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga
     4081 ttaccgcagc ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg cctggctgtc
     4141 gttcagcgac gcgccgcttc tcgataacgc agaactgcat atcgaagata atgaacgtgt
     4201 ttgtctggta ggccgcaacg gcgcaggcaa atcgacgtta atgaaaatcc tcaaccgtga
     4261 acaagggctg gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca
     4321 acaggatccg ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga
     4381 agaacaagcg gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc
     4441 gagcgagaaa aatctcaacg aactggcgaa ggttcaggaa cagctggatc accacaactt
     4501 gtggcagctg gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt
     4561 tgcgctgtcg tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt
     4621 gagcaatccg cgcgtattgc tgctggacga accgactaac catctggata tcgaaaccat
     4681 cgactggctg gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga
     4741 ccgttcgttt atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt
     4801 gacctatccg gggaattacg accagtacct gcaggaaaaa gaagaagccc tgcgcgtgga
     4861 agagctacaa aatgccgagt tcgatcgcaa actggcgcag gaagaagtgt ggattcgcca
     4921 ggggatcaaa gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg
     4981 tcgcgagcgt ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc
     5041 cagccgctcc ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa
     5101 gcaactggtg aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg
     5161 tccgaatggg tgcggcaaaa ccacgctgct gaaactgatg ctcggtcagc ttcaagcgga
     5221 cagcgggcgt attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc
     5281 ggaactggat cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat
     5341 ggttaacggc aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa
     5401 acgggcgatg acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc
     5461 gcgtttgttc ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga
     5521 tgtcgaaacg ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct
     5581 ggttagccac gatcgtcagt ttgtcgataa caccgttaca gaatgctgga tcttcgaagg
     5641 cggcggtaaa attggtcgtt atgttggcgg ttatcatgat gcccgtggtc agcaagaaca
     5701 atatgtggca ctcaaacagc ctgcggtgaa aaaaagcgaa gaagccgccg cggcaaaagc
     5761 agaaactgta aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca
     5821 gctaccgcaa ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaagtggc
     5881 ggatgcttcc ttcttcagtc agccgcatga gcagacgcaa aaagtgcttg ctgatatggc
     5941 tgctgcagag caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa
     6001 aaatggtggc tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt
     6061 gcctgatgaa ctgggtttat caggcatgaa aaccaacact ttgttagtca tctgtaaaat
     6121 agcgcatcat taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg
     6181 ctcgcagtgt gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg
     6241 tccccggtgt ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtcccaccgc
     6301 ctatgcgttg gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat
     6361 gaacgttgca ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc
     6421 tgaggactac gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt
     6481 ttgtctgata accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga
     6541 gcaactggca cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc
     6601 tggtgtgctg gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag
     6661 ctttctcccc tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg
     6721 tcgctggtta tgggacgaca tcgccccgat gccagaactg cgccaaccgc taaaaccagg
     6781 cgtcacgggg atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc
     6841 tgatgaaccc gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcct
     6901 gcagtggaca ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt
     6961 gcccatcatg gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt
     7021 cattctgtta tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat
     7081 tatggtgcca acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca
     7141 tggcaagcgc gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg
     7201 ctggtcgatg attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg
     7261 tttaatgagt atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac
     7321 aatgttttct gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga
     7381 gcatgaggag tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga
     7441 agaactggtc tcccgtgtgg atatttccta tcgtcacggc gctcattgga gcctgggttc
     7501 ttttttatca ttacagccat cagggaccgg aagtgaccct gatcaccgcg aatgcggaag
     7561 gaattgaagg tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa
     7621 gcgccacact ggctgatgat ttgacgcacg ttgaaatcaa agcgcggctg aattccggta
     7681 tggaaaaatt gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg
     7741 aagggattag cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg
     7801 cgaaaggcag caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg
     7861 atgcgaaagg tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag
     7921 atccggtgct gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacac
     7981 aaaaacgcaa tatcagctat caactgttca tcaatgcacc ttatgaccga ctggtgacca
     8041 acaatgttcg cttctggaaa gatagtggca ttgcggttga tctgacgtca gcagggatgc
     8101 gtgtggagat gggctcattg acaacgctgc tgagtggcgg tgtcagcttt gatgtgccgg
     8161 aaggtctgga tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc
     8221 agaagagcat tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag
     8281 attcggtacg cggtctgcaa ccgggagctc cggtagagtt ccggggtatt cgcctgggta
     8341 ccgtaagcaa agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc
     8401 gtattccggt actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg
     8461 cggatgttgt tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa
     8521 ccggaaacct ggtcactggt gcactgtatg ttgatctcga tttctatcca aatacgcctg
     8581 caataaccgg tattcgtgaa tttaatggtt atcagattat cccgaccgtt agcggcggcc
     8641 tggcgcaaat ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga
     8701 atccgatgat tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc
     8761 tgcaaacgac gctggatagc atgaacaaga tcctcgctag ccagtcgatg cagcagttgc
     8821 cgacggatat gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg
     8881 gctccgcagc ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag
     8941 aactgcaacc ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga
     9001 aggacaaaaa agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat
     9061 tgcagcactg tggctggtcg gatgcagctc cggcgaaatt aataaaaact attaccagtt
     9121 acctgtggtg cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt
     9181 agagcaggtc actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga
     9241 tgtgaagtat gtgattgcca acaacaactt gtgggccagc ccgttggatc aacagttgcg
     9301 caacaccctg gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc
     9361 tctgggaagc gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga
     9421 tggcaaggtc attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg
     9481 tccgttccgt ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtgct
     9541 ggccggtgtc tggagtcagg aagccgcttc tattgcacaa gagataaagc gtctacctta
     9601 attataaaga tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt
     9661 tctaacgtgc ggaaaaattt gttcctcttc acattttttg tacaaccgac atgcccgtgt
     9721 agctcacaaa tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt
     9781 attgcttaac tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt
     9841 atgagggaaa cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg
     9901 tggttatcag gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa
     9961 tcaaaggtca caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc
    10021 ctgattctgt ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc
    10081 tgtctgatta taatcagaag gcagacgtat cctggaacag accgactttc aggtcgctgg
    10141 cggtatagat cagacgacca tcaaccagca cttcgccatc cgccaggccc ataatcagac
    10201 gacggttaac aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta
    10261 cctgaccagt gaatttcact tcgccaacgc ccagcgcgcg gcctttacct tcgccgccca
    10321 gccagccgag gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca
    10381 taaccggatc gccaataaag tggcatccga agaaccacag atccggattg atatccagtt
    10441 ctgcttcaac ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt
    10501 ccatcatcag catgttcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc
    10561 gaccagaggc aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg
    10621 taagccttat tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga
    10681 tcagttcgga ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc
    10741 ctgttgcgat gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc
    10801 gccatcccac accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca
    10861 gatggtgaat ttgccttctt ctaccgcttt caccagttca ctgtgaagac ttaaatggcg
    10921 aacgttagct gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat
    10981 agcaaagaag ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg
    11041 atcgactgaa cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat
    11101 cagggcgcag agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc
    11161 aaatgtcagc gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa
    11221 cgcttgcatg atcatcatcc ctttcgtatg gatattgccg ccaagctccg ctttgcgttc
    11281 gatgtcggtg aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc
    11341 aaaagcgcgt ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg
    11401 ttcgccttcg gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc
    11461 gaggaaacct tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa
    11521 ggtgtcgcca tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct
    11581 aagcggaagt gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca
    11641 ggcatccgct cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca
    11701 ccactgggtt actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat
    11761 agcctgctct gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc
    11821 taccagaatg actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga
    11881 ctcatcaaac gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca
    11941 cagcagaggt tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg
    12001 atgcaccaga ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag
    12061 ttgctccgct tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc
    12121 tgcactcact gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac
    12181 cagttggcct gcatcgcttt gtagcgtacg cgtggcattg gcaataagat tcagatactc
    12241 agactcttcc ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg
    12301 ctccagtgcg aattgcagtc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat
    12361 caaatgtggc tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca
    12421 tgcaagtttc gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat
    12481 accgtaaccg gcactacagc acacgtagaa agcaccgaca atactcctgg catgggcgtt
    12541 aaagctcaca ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag
    12601 agttacacgg taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg
    12661 gttggaaatg gaagtatctg gttaagaagc atcgcgaagg ggagttaatc acccgttaca
    12721 tagaagccag tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg
    12781 tgctggtaaa tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc
    12841 aaaccattcg ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa
    12901 aatcgatcga tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg
    12961 gtaaaacgct ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat
    13021 acgcgaataa aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac
    13081 tgacgaaagt cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc
    13141 taccagacga gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg
    13201 atctctacgc gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg
    13261 tcacaggtgt tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct
    13321 gccgggatac ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga
    13381 ccctggttgt aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg
    13441 tctttcggat ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc
    13501 ggtttcaggg ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg
    13561 gtctgtactt ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga
    13621 ccgaaacggt aggaaacacc caggctcagc atgccgttgt ccggacgagt gccgatggtg
    13681 tgtgcgtcac cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg
    13741 atcgcgtact caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca
    13801 taaacgttgg atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc
    13861 aggtcgtcag tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat
    13921 gcaccgtttt caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca
    13981 aagccaacat acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca
    14041 tgggtcgggc cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta
    14101 gcaccagtgt accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc
    14161 agtgccactg caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa
    14221 atacgccatg aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct
    14281 caatattaac tctaccgata tcttcggctt atgccgagca cccctggcga tgtaaagtct
    14341 acaacgtagt tgaaaactta caagtgtgaa ctccgtcagg catatgaaaa aaaagtcttg
    14401 tataaggtat g
//
LOCUS       DAZKIV010000118        14374 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZKIV010000118 DAZKIV010000000
VERSION     DAZKIV010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.118"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..1177
                     /gene="tsuA"
                     /locus_tag="ACHOT6_003464"
     CDS             119..1177
                     /gene="tsuA"
                     /locus_tag="ACHOT6_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFP9948627.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            1191..1418
                     /gene="tsuB"
                     /locus_tag="ACHOT6_003465"
     CDS             1191..1418
                     /gene="tsuB"
                     /locus_tag="ACHOT6_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFP9948628.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1461..2888)
                     /gene="sbcB"
                     /locus_tag="ACHOT6_003466"
     CDS             complement(1461..2888)
                     /gene="sbcB"
                     /locus_tag="ACHOT6_003466"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFP9948629.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMNPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADDKEKVALLKALWQYAEEIV"
     gene            3097..4263
                     /gene="dacD"
                     /locus_tag="ACHOT6_003467"
     CDS             3097..4263
                     /gene="dacD"
                     /locus_tag="ACHOT6_003467"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFP9948630.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPQIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4382..4855
                     /gene="sbmC"
                     /locus_tag="ACHOT6_003468"
     CDS             4382..4855
                     /gene="sbmC"
                     /locus_tag="ACHOT6_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFP9948631.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            5053..6111
                     /gene="yeeA"
                     /locus_tag="ACHOT6_003469"
     CDS             5053..6111
                     /gene="yeeA"
                     /locus_tag="ACHOT6_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFP9948632.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGAILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTC
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            6283..6612
                     /gene="tmaR"
                     /locus_tag="ACHOT6_003470"
     CDS             6283..6612
                     /gene="tmaR"
                     /locus_tag="ACHOT6_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFP9948633.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(6713..6904)
                     /locus_tag="ACHOT6_003471"
                     /pseudo
     CDS             complement(6713..6904)
                     /locus_tag="ACHOT6_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
     gene            7071..7616
                     /gene="cobU"
                     /locus_tag="ACHOT6_003472"
     CDS             7071..7616
                     /gene="cobU"
                     /locus_tag="ACHOT6_003472"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFP9948634.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            7613..8356
                     /gene="cobS"
                     /locus_tag="ACHOT6_003473"
     CDS             7613..8356
                     /gene="cobS"
                     /locus_tag="ACHOT6_003473"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFP9948635.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            8368..9447
                     /gene="cobT"
                     /locus_tag="ACHOT6_003474"
     CDS             8368..9447
                     /gene="cobT"
                     /locus_tag="ACHOT6_003474"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFP9948636.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGCGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            9509..10435
                     /gene="ldtA"
                     /locus_tag="ACHOT6_003475"
     CDS             9509..10435
                     /gene="ldtA"
                     /locus_tag="ACHOT6_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001773987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFP9948637.1"
                     /translation="MMRRVNIFCSFALLFASHNSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVNINRNGF"
     gene            10609..11574
                     /locus_tag="ACHOT6_003476"
     CDS             10609..11574
                     /locus_tag="ACHOT6_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HFP9948638.1"
                     /translation="MTKQKKNSRWQGYDPRYIYSLVVRRYFADMKTKIEIAEELGVSR
                     FKVARLIDEAIEQDYVRFIFPKQQAMDEEIASNLRKKFHLEDAIVLSVAESWTTQEEL
                     NHKLGEVTAEYLMQSIHEGMKVGIAWGRVLSSTVSKLSKLPPLDVVQLSGVHPGIEFS
                     QGPIDLIHKIAAISQGKAHPMYVPMWVDDEELAARLAGEPAVLDTQQYYSQLDVVITG
                     IGDWKSGSSSLYKIFPDTWCEALFQQDIAADVCISLVNREGKILHSPIERLGFGISTD
                     QLQKAKKVIGVAGGEEKYEGILASLKSGLLNVLITDFDTAIKLLD"
     gene            complement(11626..12084)
                     /locus_tag="ACHOT6_003477"
     CDS             complement(11626..12084)
                     /locus_tag="ACHOT6_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9948639.1"
                     /translation="MKFEILVNDVDNSISDYQTAITFAGTQLLEKGYITAEYIDACLE
                     REKSYPTGLMMANGQGIAIPHADYTLVNVNSISIVRFANDVTFGQMEDADLTVDCSIM
                     FNLAFATSDQHMSILRRLFTLFQDNAFVETCRNLPATRVAKYVEDTLAAS"
     gene            complement(12095..12379)
                     /locus_tag="ACHOT6_003478"
     CDS             complement(12095..12379)
                     /locus_tag="ACHOT6_003478"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9948640.1"
                     /translation="MKKILVVCGNGIASSSIMVSVLQDFLKEEKIEAQVDKSSLMACT
                     SDTFNSYDLVVSSTKLDNPTITTRVIVGAGLLTGLGEDEIFAAVKEEMLK"
     gene            complement(12403..13774)
                     /locus_tag="ACHOT6_003479"
                     /pseudo
     CDS             complement(12403..13774)
                     /locus_tag="ACHOT6_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564759.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
     gene            complement(13778..14314)
                     /locus_tag="ACHOT6_003480"
     CDS             complement(13778..14314)
                     /locus_tag="ACHOT6_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HFP9948641.1"
                     /translation="MKTKIAIASDDVGFARKEEIKKYLIEEKNMEIVYDPVTCKEQGV
                     NNFARLADEMAGIIQRDECRLGIYICGTGIGFTCQINKHWGIRAVAVTNPYSAKRARL
                     SNNAQVIGLGCRVNDLEYSKMIVDAWLDNPFDFTTARENSKKNLLEAERNDNALLVKP
                     ADVRWNMGFRPDDEKSEG"
BASE COUNT         3615 a         3220 c         3685 g         3854 t
ORIGIN      
        1 tttttatcga catcctcatt aataaccatt tgttctaatt tagagaattt ggttattggt
       61 ctgcattcca ccagtcatga ataatttccc tattaacatt catctggtta attaatttat
      121 gttttcaatg atattaagcg ggctaatttg tggtgctctg ctgggatttg tcatgcagcg
      181 tgggcgtttt tgcctgacag gtggttttcg cgatatgtat ctcgcgaaaa ataatcgcat
      241 gttttacgcc ttgctgattg caatttcggt acaaagtgtg ggggtttttg cgttaattca
      301 ggcgggccta ctgacttacg aagccggggc gttcccgtgg cttggtactg ttataggtgg
      361 gtatatcttt gggctgggaa ttgttctggc gggtggatgt gccaccggga cctggtatcg
      421 cgcgggtgag ggattgatcg gcagttggat cgcgcttttc acttatatgg tgatgagtgc
      481 ggtgatgcgt tctccacatg ccagtggttt aaatcaaacc ttgcagcact acagtactga
      541 acataactct attgctgaga cttttaattt gtctgtgtgg ccgttggttg ccgttttgct
      601 ggtgataacg ctctgggtgg tgatgaaaga gttgaagaag ccaaaactca aagtcgcgac
      661 cttaccgccg cgccgaaccg ggatcgctca tattctgttt gaaaaacgct ggcatccctt
      721 tgtcactgct gtactcatcg gtttgattgc gcttttagcc tggcccctga gtgaagcaac
      781 cggacgcatg tttgggttag gaatcacttc cccaacggcc aatattctgc aatttctggt
      841 cgcgggtgac gtgaaataca ttaactgggg cgttttcctg gtgttaggga tcttcgtggg
      901 gtcatttatt gcagccaaag cgagccgtga gttccgcgtt cgcgcagctg atgcacaaac
      961 aacattacgt agcgggttag gtggtgtact gatgggcttc ggtgccagta ttgcaggtgg
     1021 ttgctctatc ggtaatggac tggttatgac tgcaatgatg acctggcagg gctggattgg
     1081 ccttgtattt atgattctcg gagtctggac tgcgtcctgg cttgtgtatg ttcgaccgca
     1141 gcgtaaggcg cgactggcaa ctgctgcggc aaattaagta aggtgtaaag atggcgatta
     1201 aaaagctgga tgtggtgacg caggtctgtc cgtttccact catagaagca aaagcagcac
     1261 tggcagaaat ggccagcggc gatgagttgg taattgagtt tgattgcacc caggcaactg
     1321 aagcaatccc acagtgggcg gcagaagaag gacatgccat caccgattat cagcagattg
     1381 gcgatgcagc gtggagcatc accgttcaaa aagcctgact caacattgtc ctccgccgta
     1441 ccagcggcgg aggcttcaaa ttagacgatc tcttccgcgt actgccaaag cgcttttaac
     1501 agcgccactt tctctttgtc atcggcatat tgttgtgcca gcatctgcaa ttcatctgca
     1561 taaccctgta aaaactctgg cgtgaagacc tggcggcggt gctccagcca gcgttgctgc
     1621 tcggcataat ccagcgtccc cgggaagttg cgcgcccgat aattgaacaa cagcttttca
     1681 atccgtttat cgacaaaagt gatatccagt gccggtaaat tacgcggctc ggtttccagc
     1741 acaattttca ttgctgcacg atcggcgtca ctgaaaaagc cgttataaag ctgtgcatcc
     1801 acgttatctg aaggcgtaaa cggttcggct tccgcgaata tcgccaccac tttttcgcgc
     1861 acttgcggat tttcacgcag aattttcagg ttatcgaggc aatgctgacg attaattccc
     1921 agtcggtcgg catcttccgg gcgtagcgta ttcgcctggg ccagcaccgg acatttattg
     1981 atatgcacca gcttaaccgg aacggcggcg ttatcgccaa gatcggtttt tgcggtatat
     2041 aaacgctcgc gcaatgtgtc gctatccagt tccagtaatg gcgaaatgtc tcctgccaaa
     2101 tccaccataa ttacggcatt gcgattttca ggatgccacg ccagcggtgc cacccagctg
     2161 gtattgccgc gccatgctcc aaacattccg gaaacgtgca ccagggggtt catctgcgga
     2221 acatcgatca acgccatcag tttgtgttta ttacgatggg taaagagata atcaaacagg
     2281 cgtggctgac gcgtttttac cagctttgcc atcgcaatag tggcgtacac atcagccatc
     2341 gcatcgtggg cgttgctatg ttcaatacca ttcgctttgg ttaaatgctc aaggcgaaag
     2401 ctcggtagac cgtcatcatt ttcaggccag tttattcctt ccgggcgcag ggcataacag
     2461 gcacgcataa catccagcaa atcccagcgc gagttatcat gctgccagct ccaggcgtaa
     2521 ggatcgtaga aattacgata aaaaacgttg cgtgtgactt cgtcgtcgaa acgcacattg
     2581 ttgtagccca gaatacaggt cttcggtacg gtaaaaagcg agtgaatacg ggcggcaaac
     2641 gcggcttcgt tttctccttt cgcccgtgct tcctgcgggg taataccggt aattaatacg
     2701 gctcctggct ggggtaaata gtcatcagcg ggcttgcagt aaaagacttc gggttcgccg
     2761 atgacattga attcgctatc ggtgcgaatg gctgcgaact gtgcagggcg atctaacgcg
     2821 gggtgcgtgc caaaagtttc gtaatcgtga aacaaaaagg tagattgttg cttaccgtca
     2881 ttcatcatta ggttaaatcc gttatttctg ttgtctgcca gagtatcaaa tatcaccgtg
     2941 ctaatcagct ttagcgcaac aatttgacag cgagtggcaa cagatcatgt cagataaaaa
     3001 tgagagagta gtcacatttt cttgcacttt attccagcca gttcataagt atttccgtaa
     3061 aaagaacagc tatttgaaac tcctgagggt ttgctgttga aacgccgtct tattattgct
     3121 gcttctttgt tcgtttttaa cttatcgtct ggttttgcgg cggaaaacat tcctttttca
     3181 cctcagcctc cacagattca tgccgggtcc tgggtactga tggattacac caccggtcag
     3241 atcctcaccg cgggtaatga gcatcaacag cgcaatcccg ccagcctgac aaagctgatg
     3301 acgggttatg tcgtggatcg cgctatcgat agtcatcgca ttacgccaga cgatattgtc
     3361 accgtggggc gcgatgcgtg ggcgaaagat aatccggtgt ttgtcggttc ttcactgatg
     3421 tttttgaaag agggcgatcg cgtatcggta cgtgatttaa gccgtggttt aattgtggat
     3481 tccggaaatg acgcttgtgt tgctctggct gactatattg ccggtgggca acggcagttt
     3541 gttgaaatga tgaacaacta tgccgagaag ctgcatctca aggatacgca ttttgaaaca
     3601 gtgcatggtc tggatgcacc tggccagcat agctcggctt atgatttagc tgtgctttct
     3661 cgcgctatca tccacggcga gcccgagttt tatcatatgt acagtgagaa aagtctcacc
     3721 tggaacggta tcacccagca aaaccgtaac gggttgttgt gggataaaac catgaatgtt
     3781 gacggcctga aaacgggtca tacttctggt gccggtttta acctcattgc ttcggctgta
     3841 gatgggcagc gtcgtctcat tgcagtggta atgggggctg acagtgcaaa aggtcgtgag
     3901 gaagaggcaa gaaaattact gcgttggggg caacaaaact ttactacggt gcaaattttg
     3961 caccgtggga aaaaggtcgg tacggaacgc atctggtatg gcgataaaga aaatatcgcc
     4021 ctgggaacag aacaagagtt ctggatggta ctaccgaaag ccgaaattcc acatatcaaa
     4081 gccaaatata cccttgatgg taaagaactc accgcgccaa ttagcgccca tcagcgggta
     4141 ggggaaattg aactttacga ccgtgataaa caggtggcgc actggccgct ggttaccctg
     4201 gaatctgtcg gggaaggcag catgttttct cgcctgagtg attatttcca ccataaggct
     4261 tgacctttct tttgcagcag actggcagga gtgcgagtct gctcgcataa tcaacactca
     4321 ttccttgtgg ttttaatatt gcaactatac tgtatataaa aacagtatta atggaggcgt
     4381 catgaactac gagattaagc aggaagataa acgtaccgtt gcaggtttcc atctcgttgg
     4441 cccctgggaa cagacggtaa agaaaggttt tgagcagttg atgatgtggg tagataacaa
     4501 aaatattgtg ccgaaggagt gggttgctgt ctattacgac aatccagatg aaacacccgc
     4561 cgaaaaatta cgctgcgaca ccgttgtgac ggtaccgaat aactttacgc ttcccgaaaa
     4621 cagtgagggc gtcattctga cagaaatttc aggtggtcag tatgcggtgg cggtggctcg
     4681 tgtagtcggt gatgattttg ctaaaccctg gtatcagttc tttaatagcc tcttgcagga
     4741 cagtgcttat gaaatgttac caaagccctg cttcgaggtt tatttgaaca atggcgcgga
     4801 agatgggtat tgggatatcg aaatgtatgt tgcggtgcag ccaaaacatc actaattcat
     4861 ctcagggcgg tgtgttaacg cgatgaccac tctttttttg aaagcgaaaa gagtaagatg
     4921 cgcctttcaa ttttttcgct cctgccggga aattacactg ttcccggttt gtccgtcgga
     4981 taattcagag gcgcgccttc tggccgacag atgagttatg agcgctttta atcttcatta
     5041 cggagtttct gcgtgcgtgc cgataagtca ttaagcccgt ttgaaatccg ggtataccgc
     5101 cattaccgca ttgtgcatgg tactcgggtc gcgctggcat tcctgctcac ttttctcatt
     5161 atccgcctgt ttactatccc tgaaagcacc tggccgctgg tcaccatggt ggtgattatg
     5221 gggccaatct cgttctgggg taacgttgtt cctcgcgcct tcgaacgtat tggcggtacg
     5281 gtgttggggg cgattttagg tcttatcgct ctgcaactgg agttaatttc gttaccgctg
     5341 atgttagtct ggtgcgcggc ggctatgttt ctttgcggtt ggctggcact gggcaagaaa
     5401 ccgtatcaag gattattgat tggggtgacg ctggcaattg ttgtgggttc cccgacaggt
     5461 gaaattgata cggcgttatg gcgaagcggc gatgtgatcc tcggctcttt actcgcaatg
     5521 ttgtttacct gtatctggcc acaacgggcg ttcatccact ggcgcattca actggcgaaa
     5581 agtttgaccg agtataatcg ggtttatcaa tctgcattct caccgaactt actcgaacgc
     5641 ccacgtctgg aaagccatct acaaaaactc ttgaccgatg ccgtgaaaat gcgtgggctg
     5701 attgcgcccg ccagcaaaga aactcgtatt ccaaaatcga tatatgaagg tatccagacc
     5761 attaatcgca atctggtttg tatgctggag ttgcaaatca atgcgtactg ggccacgcgc
     5821 cccagccatt tcgtgttatt gaacgcgcaa aaacttcgtg acacccagca catgatgcag
     5881 caaatactgc tgagccttgt tcatgcgctg tacgaaggta atccgcagcc ggtttttgcc
     5941 aatacggaaa aattgaacga tgctgtggaa gagctgcgtc agttgctcaa taaccaccat
     6001 gacctgaagg ttgtggaaac accaatctat ggttatgtgt ggctgaacat ggagacggca
     6061 catcagcttg agttgctatc gaatctgatt tgccgggcct tgcgcaaata attcctgaac
     6121 ttcagaatca tcttgctgct gcttcgattc agcaaggata aagggtatga tagtgaaaag
     6181 ggataaaagc attgtcatct gcggcagcta tgagtaatgt tggccctaac gaatagcggt
     6241 tgcttaaacg aatccgactc tcacattatc aggggtataa aaatggaaac taccaagcct
     6301 tcattccagg acgtactgga atttgttcgt ctgttccgtc gtaagaacaa actgcaacgt
     6361 gaaattcagg acgttgagaa aaagatccgt gacaaccaga agcgcgtcct gctgctggac
     6421 aacctgagcg attacatcaa gccggggatg agcgttgaag caatccaggg catcatcgct
     6481 agcatgaaag gtgactatga agatcgcgtt gacgattaca tcatcaaaaa tgccgagctc
     6541 tccaaagaac gccgcgatat ctccaaaaag ctgaaagcta tgggcgaaat gaaaaacggc
     6601 gaagcgaagt aattcctgtt ttattcaatg agggttgcct ggcaaccctc attgctcatt
     6661 gattcttttc tgtgtatcac agtcatcatt cccatctgat aacctatcga aattagcgac
     6721 agacatcttc tgtatgcagc aaggcaaaaa gttctgtaac tccatggtta ttaaccgcat
     6781 tggttactaa cacgttgtgc gctccggctt cccgtaacca acatttcacc aaagatattt
     6841 gttccatgct ggctaaatct gctttggtga ctactccaat gatcggacgt taacacgagg
     6901 aagtctggct attccacaat gccatcttct ttcgtaatgg attgagaatg ctggattttt
     6961 aattatatta cgcgcagtat taatcgcgta aatgttttgc ggtcagcaac ggtgggggat
     7021 ccattgttct gttcaatgaa taccatcata caattgaatc aggagtcggt atgatgattt
     7081 tggtgacagg tggagcacgg agcgggaaga gtcgccacgc agaggcgctt attggggact
     7141 cttcacaggt tctgtatatc gccacctcgc aaatccttga tgatgagatg gctgcacgga
     7201 tagaacatca tcggcaaggc cgcccggcgc actggcgcac ggtggagcgt tggcaacatc
     7261 ttgatgaatt aattcatgca gacattaacc cgcatgaagc tgtgttgctg gaatgcgtta
     7321 ccacaatggt gactaatctg ttgtttgatt atggcggcga taaagaccct gatgaatggg
     7381 attatcaggc gatggaacag gcgattaatg ctgagattca gtcattgatt gctgcctgcc
     7441 aacgttgccc cgcaaaggta gtattagtga ctaacgaagt gggaatgggg attgtgccgg
     7501 agagtcgtct ggcacgacat tttcgtgata ttgccgggcg ggtaaatcag cagctggccg
     7561 ctgcggctaa tgaagtatgg ctggtggttt cgggtattgg agtaaaaatc aaatgagtaa
     7621 attattttgg gcgatgctct cttttattac gcgtctgcct gtaccgcgtc gctggtccca
     7681 gggactggat ttcgagcatt attctcgcgg tattattact tttcctttaa ttggattatt
     7741 gcttggcgcg attagcgggc tggtcttcat ggtgctgcag gcatggtgtg gcgtaccact
     7801 ggcggcactg tttagcgtac ttgtgctggc gctgatgacc gggggattcc acctggacgg
     7861 cctcgccgat acctgtgatg gcgtattttc tgcacgtagc cgcgatcgca tgttggaaat
     7921 catgcgtgat agccgtttag gcacccacgg cggtctggca ttgatttttg ttgtactggc
     7981 aaagattctg gtgttaagcg agttggctct gcgtggcgaa ccgatccttg catcgctggc
     8041 ggcagcatgt gcggtcagcc gcgggactgc cgcattattg atgtatcgtc atcgttatgc
     8101 ccgggaagaa gggcttggca atgtgttcat tggcaaaatt gatgggcgac aaacctgcgt
     8161 cacactcggc ttagccgcta tttttgctgc cgtattattg ccgggtatgc atggtgtggc
     8221 tgctatggtg gtgacgatgg tggcgatttt catcctcggt cagcttctca aacgtacgct
     8281 gggcgggcaa accggcgata cgctgggagc tgctatagag cttggtgaac tggtcttcct
     8341 gctggcactg ctataaagag acgtgacatg caaacacttg ccgatttact gaatacgatc
     8401 cctgccatcg atcctgccgc tatgtcgcgt gcacaacggc atattgacgg gttactcaaa
     8461 cctgttggta gcctgggaag gctggaggcg cttgccatac aactggcagg aatgccgggg
     8521 ttgaatggca taccgcatgt gggcaaaaaa gcggtactgg ttatgtgtgc cgatcacggc
     8581 gtctgggagg aaggggtcgc tatttcccca aaagaagtga cagccattca ggctgaaaat
     8641 atgacccgcg gaacaaccgg cgtgtgtgtg ctggcagcac aagcgggcgc taacgtccac
     8701 gtaattgatg ttggtattga tactgctgag cctatccccg ggcttatcaa catgcgtgtc
     8761 gcacgaggtt gcggcaatat tgcttcggca ccggcaatga gtcgccgtca ggctgaaaag
     8821 ttgcttttgg acgtcatatg ttatacgcgg gagctggcaa aaaacggtgt cacgctgttt
     8881 ggtgtaggtg aactggggat ggcaaacacg acaccggcag cagcaatagt cagcacaatc
     8941 actggccggg atcctgaaga agtggttggg attggcgcaa acctgccgac agataaactg
     9001 gctaataaaa ttgatgttgt gcgccgggcg attacgttga atcaaccaaa tcctcaggat
     9061 ggcgttgatg tcctggcaaa agtgggtgga tttgatttgg tcggaatggc tggcgtgatg
     9121 ttaggcgctg cttcctgcgg tttacccgtg ttgctggatg gatttctttc ttatgctgcc
     9181 gcgctcgcag cctgccagat gtctcctgca atcaaaccgt atctcattcc ttctcacctg
     9241 tcggcagaaa aaggggcgcg tattgcgctg tcgcatttgg ggctggagcc ttatctcaat
     9301 atggagatgc gtttaggtga ggggagtggc gcagctctgg cgatgcccat tatcgaagct
     9361 gcctgtgcga tatacaacaa catgggcgaa cttgctgcca gtaatattgt tctaccgggg
     9421 aatacgactt ctgatttgaa cagttaaaaa cgaaaagtct gataaaagct atacttaacg
     9481 gatagctttc gcgacttagg aaagggacat gatgcgtcgt gtaaatattt tttgctcatt
     9541 tgctctgctt tttgccagcc ataatagcct ggcggtaact tatccattac ctccagaggg
     9601 tagccgttta gtggggcagt cgtttactgt aactgttcct gatcacaata cccagccgct
     9661 ggagactttt gccgcacaat acgggcaagg gttaagtaac atgcttgaag cgaacccggg
     9721 cgctgatgtt tttttgccga agtctggctc gcaactcacc attccgcagc aactgatttt
     9781 gcccgccact gttcgtaaag ggattgtggt taacgtcgct gagatgcgtc tttattacta
     9841 cccaccagac agtaatactg tggaagtctt tcctattggt atcggccagg ctgggcgaga
     9901 aaccccgcgt aactgggtga ctaccgttga acgtaaacaa gaagcaccaa cctggacgcc
     9961 aacgccgaac actcggcgcg aatatgcgaa acgaggggag agtttgcccg catttgttcc
    10021 tgcggggccc gataatccca tggggctgta cgcgatttat attggcaggt tgtatgccat
    10081 ccacggtacc aatgccaatt ttggtattgg gctccgggta agtcagggct gtattcgtct
    10141 gcgcaatgac gatatcaaat atctgtttga taatgttcct gtagggacgc gtgtgcaaat
    10201 tatcgaccag ccagtaaaat acaccactga accagatggc tcgaactggc tggaagttca
    10261 tgagccgttg tcgcgcaatc gtgcagaata tgagtctgac cgaaaagtgc cattgccggt
    10321 aaccccatct ttgcgggcgt ttatcaacgg gcaagaagtt gatgtaaatc gcgcaaatgc
    10381 tgcgttgcaa cgtcgatcgg gaatgcctgt gaatatcaac cgtaacggat tttgacattc
    10441 aaaacgtctg gtttgtcggt ctgtgtaagc attacgtcat gcagaacagg ttgtttcgaa
    10501 tcatacaaat gataattttc ttctcatatg cgcatctctt atgagcataa tcgttatact
    10561 cttctgattt atacatgttt gctgatgaca tgatcaatgg aaaagagaat gacgaaacag
    10621 aaaaaaaaca gccgctggca gggttacgat cctcgctaca tatactcgct cgtggtcaga
    10681 cgttattttg cggatatgaa aaccaagatt gagatcgcag aggagcttgg cgtttcccgc
    10741 tttaaagttg ccagattgat cgatgaggcg atcgaacagg attacgtgag gtttatcttc
    10801 cctaagcagc aagcgatgga tgaagaaatc gccagtaatc tacggaaaaa atttcatctg
    10861 gaagatgcaa ttgttctttc agttgctgaa tcctggacga cgcaagaaga actcaatcac
    10921 aaattgggtg aagtcaccgc tgaatatctt atgcaatcta ttcatgaagg tatgaaagtg
    10981 gggatcgcct gggggcgtgt attatcgagc acggtcagta agttgagcaa gctgcctcct
    11041 ttagacgttg tgcagttatc tggcgtacat ccggggatag agtttagtca agggccaata
    11101 gatcttattc ataagatcgc tgccatttcg cagggaaaag cgcacccaat gtacgtgccg
    11161 atgtgggtcg atgacgaaga gcttgctgcc agactggcag gtgagcctgc ggtattagat
    11221 acacagcaat attactcaca gttggatgtg gttatcaccg ggataggtga ctggaaatca
    11281 ggttcttcaa gcttgtataa aatatttccg gatacctggt gcgaagcttt gtttcaacaa
    11341 gatatcgctg cggatgtgtg tatctcgttg gtcaacaggg aagggaagat tcttcatagt
    11401 cctattgaac gtctgggatt tggcatttcg acggatcaac tacaaaaagc caaaaaagtg
    11461 attggtgtcg ctggaggaga agaaaaatat gaaggcattc ttgcttcgct gaaatccgga
    11521 cttttgaatg tcttaattac tgattttgat acggccatta aacttctgga ttaatagccg
    11581 tatcagtgaa gaaagggcag taccgctgat tctgcccttt taatttcagg atgctgcgag
    11641 cgtatcctct acatatttag ccacgcgcgt tgcaggtaaa ttgcggcaag tctcaacaaa
    11701 ggcgttatcc tgaaacagtg tgaataaacg acgcaatatc gacatatgtt gatcactggt
    11761 ggcaaacgcc aggttaaaca taatgctaca atcaactgtt aaatcagcat cttccatttg
    11821 cccgaaagtg acatcattgg cgaaacgaac aatactgata ctgtttacat ttaccagcgt
    11881 gtaatcggca tggggaatgg cgataccctg accgtttgcc atcattagcc ctgtcggata
    11941 gcttttttca cgttccaggc aggcgtcaat gtattctgca gtgatatagc ctttttccaa
    12001 taattgcgta ccagcaaagg tgatcgccgt ttgatagtca ctgatgctgt tatcaacatc
    12061 attcaccaga atttcaaatt tcattgcaca cctgttattt caacatctct tctttaactg
    12121 cagcgaaaat ctcatcttcg cccagaccag tcagtaatcc agcgccaacg atgacacgag
    12181 tagtaatggt cgggttatcg agtttggttg atgaaaccac caggtcatag ctgttgaagg
    12241 tatcgctggt gcatgccatt aacgaagatt tatcgacttg cgcttcaatt ttctcttctt
    12301 tcaggaagtc ctgcagtaca gagaccatga tggaagatga ggcaattccg tttccgcaaa
    12361 ctactaagat ttttttcatt tgattattcc tctgtaaaag atttaaagcg ctttttccaa
    12421 atccgtttca atctggcgac gtgatttttg tttaattaag tatccaatag ataacaaaag
    12481 gatacctgcc atcgcatagc caatgattcc aacatggctc agggcggcaa acagaccagt
    12541 aaatggattc gctgacgggt taagcgaaga gtacatcacg ctggcatccg ggtttgtacc
    12601 aaacttctgg aagacttcgt tggtagccgg tgccatccac gatgcgatat acagaccacc
    12661 accgaacaaa atgatcgagg tcacccaggt gcgaataatg ttgccattaa aaacaggcac
    12721 catgcaggca atgaaaaaca caaactgaga gagatcaccc agtggcataa cgcggttgcc
    12781 gggcaagatc acggcgagga tcacaatcac cgggatcagc aataatgcag gggcgataac
    12841 tgatggatgg cccacaatca gagcagtgtc cataccaaca gtgatttgac gatccgggaa
    12901 acgtttgacc agttttttgc gtgctacgtt ggaaagcgga acaagccctt ccatcatgat
    12961 agaggtcata cgtggcaaca gaagcatgac tgcagcaaga tacatcccta actgtaggct
    13021 ggcgattatg gtgtggtatg gggatttcca gccataacca caaaagccca acaaaatacc
    13081 aataataaaa ccaaccatag ttggatcgcc gagaatgcca aaccgttttt gaatagtttc
    13141 ggcggtcaca ttcagattac ggaagcctgg gatgcgatca taaatccagt tgaatggata
    13201 agcgaaaagt aagtaggtat tggcaaccgg atgagagatc gcaacacctt ttggcaggtt
    13261 aaagaatttt ttcgttattt ttagcaccaa gatcggcagc gatcagcgcc cagacatgga
    13321 atagcgcggc ggtcaatacg ccatacagga agctaccaga atagacgcca acaattgccc
    13381 ccattgacgc attttgatga aggttgaaaa catcaacgtt aacacagtca gtcaggcggg
    13441 cgaccagcat tgcaatattt accgcaacac agaggggaat aatgatagcg cccatgctgg
    13501 agccaaatcc cataaccgca gtcgctgcgc caccaacatc gatggcactg agcgaggaac
    13561 ctgttgcttc tgctaatccg gtgactgccg gttgcatttt ttccagcatc agtttgatgg
    13621 tcatggttaa cgcaatgaaa ccaatgccga ctttgatggc agacgagata cattctgaaa
    13681 gaggacggcc aagtatcttc atcagtacca gaaagatgac cgggataaat ataaaatttc
    13741 ccagatcgtt tataaattgc gtgatcagtt ccatacatta accctcagat ttttcatcat
    13801 ctggtcggaa acccatattc cagcgtacat cagcgggctt aaccagcaat gcattatcgt
    13861 tacgttcagc ttctagcaag ttctttttag aattttctct ggcggtggtg aagtcgaatg
    13921 ggttatccaa ccatgcatcg acaatcattt tgctgtattc gagatcgttg acccggcaac
    13981 ccagtccaat aacttgtgcg ttattgctga gtcgcgcccg ctttgcggag taggggttag
    14041 taaccgcaac ggctcttatg ccccagtgtt tgttgatctg acaagtaaag ccaatgccag
    14101 tcccacaaat gtaaattccc agacggcact catcgcgttg aataatgcct gccatttcgt
    14161 cggccagacg cgcgaagtta ttgacacctt gttccttaca tgtgacggga tcgtacacga
    14221 tctccatgtt tttctcttct atgaggtatt tttttatctc ctctttacgt gcaaaaccta
    14281 catcatcgct tgcgatagca atctttgttt tcatttcatt ttgcctgtcg ttaagtaact
    14341 aaagccaagt atgctgttgg taaatattgg ttgt
//
LOCUS       DAZKIV010000119        14238 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZKIV010000119 DAZKIV010000000
VERSION     DAZKIV010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.119"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..556
                     /gene="ykgH"
                     /locus_tag="ACHOT6_003481"
     CDS             <1..556
                     /gene="ykgH"
                     /locus_tag="ACHOT6_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YkgH"
                     /protein_id="HFP9948642.1"
                     /translation="FLYINKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDV
                     IQRITTFFQTSPDVKNREIRLEWSGDKRALPTAEEEISRVQASIIKWYASEYHNGRQV
                     LDEIQTPSAINSELYTKMIYLTRNWPLYPNGDGCVTISSPEIKNKYPAAICLALGFFL
                     SIVISVMFCLVKKMEDEYQQNSGQ"
     gene            complement(799..1494)
                     /locus_tag="ACHOT6_003482"
     CDS             complement(799..1494)
                     /locus_tag="ACHOT6_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFP9948643.1"
                     /translation="MDNRGEFLNNVAQALGRPLQLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     KSSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1487..2913)
                     /locus_tag="ACHOT6_003483"
                     /pseudo
     CDS             complement(1487..2913)
                     /locus_tag="ACHOT6_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
     gene            complement(2924..3643)
                     /locus_tag="ACHOT6_003484"
     CDS             complement(2924..3643)
                     /locus_tag="ACHOT6_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFP9948644.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKIVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4170..5024)
                     /gene="rclR"
                     /locus_tag="ACHOT6_003485"
     CDS             complement(4170..5024)
                     /gene="rclR"
                     /locus_tag="ACHOT6_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFP9948645.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5250..6575
                     /gene="rclA"
                     /locus_tag="ACHOT6_003486"
     CDS             5250..6575
                     /gene="rclA"
                     /locus_tag="ACHOT6_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFP9948646.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEANLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
     gene            6684..6920
                     /gene="rclB"
                     /locus_tag="ACHOT6_003487"
     CDS             6684..6920
                     /gene="rclB"
                     /locus_tag="ACHOT6_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFP9948647.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6932..7525
                     /gene="rclC"
                     /locus_tag="ACHOT6_003488"
     CDS             6932..7525
                     /gene="rclC"
                     /locus_tag="ACHOT6_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFP9948648.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWKTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEVWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7685..8554)
                     /locus_tag="ACHOT6_003489"
     CDS             complement(7685..8554)
                     /locus_tag="ACHOT6_003489"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFP9948649.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGELVKHFNP
                     EFVRGCLAVKIHD"
     gene            8803..9660
                     /locus_tag="ACHOT6_003490"
     CDS             8803..9660
                     /locus_tag="ACHOT6_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9948650.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPAKGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            complement(9781..14034)
                     /gene="fdeC"
                     /locus_tag="ACHOT6_003491"
     CDS             complement(9781..14034)
                     /gene="fdeC"
                     /locus_tag="ACHOT6_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFP9948651.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQTDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQKPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSGQSTVSVAAD
                     RVKAGESTTVTLIAKDAHGNAISGLSLSASLTGAASEGATVSGWTEKGDGSYVATLTT
                     GGKTGELLVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGLKPDAPVFSGAASTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLTLPQGVTSKTGNTVTTNAAGKVDIELMSTVAGEHNISASVNGAQKTVTVKF
                     NADASTGQANLQVDAPAQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITALAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         3706 a         3574 c         3130 g         3828 t
ORIGIN      
        1 gtttctgtat atcaataaag acaatataaa agtgatttac agcctaaaaa taaaccagac
       61 aacgccaggt atacttgtta gctgtgatag caataataat tttgcctgtc agaccacaat
      121 gactgaagat gttattcagc gaattactac attttttcag accagcccag atgtcaagaa
      181 cagagaaata aggctggaat ggtcaggaga taagagagct ttaccaactg ctgaagagga
      241 aatatctcgc gtgcaggcct ctattatcaa atggtatgcg tcagaatatc ataatggcag
      301 gcaagttctc gatgagatac aaacgccttc agcaattaac agtgagcttt atacaaaaat
      361 gatatacctg accaggaact ggccattgta tccgaacggt gatggctgtg taactataag
      421 ctcaccagaa ataaaaaata aataccctgc tgccatttgc ctggctctgg gattttttct
      481 aagcattgta atttctgtaa tgttttgcct tgtcaaaaaa atggaagatg aataccaaca
      541 aaactctggg cagtaataaa cagcattctg atagagagtg cctgtataaa gctgaagtga
      601 caatgaccgg gaagggaacc cgttttcagg aatggatgcc tgtctttttc actgtaaata
      661 tcctggcatt actaatgcca ggatatttac agaagagcag cataaccatg acggaacggg
      721 gttaagtcag gttgtcctta acgctatttt caacctcgta tgccttcttc aggtttatgt
      781 tcagatctca tatctctctc agcaatcctc aataatcaga tacaccgctt tcactggacc
      841 atgaaccccg acgactttga taagctcaat atccgccgtt gaactagggc cgctaatgat
      901 gttaatgcag gacggcattc gttcaccggc ctgcgctttc tgatgcaatt tttctgcgag
      961 ttgcgctaca cgcggcagga ttgtgctttt acgcaggata aaaagagaag atttcgggag
     1021 caggctcaat gaacgcccgc gctcggcggc ggaaaaaaga accacgcctc ccgattcggt
     1081 taaaccatat tcagcataca cgacacccac tttagcctgc tctgcctgcg agatattctc
     1141 ggcacctttc gccggatccc aaacaacggc attgcattcc tgctgcaaac gttcactaat
     1201 ccccaattcc tccagcctcg tgtcaccgct aatcacgacc gactgatctc ccagctcttt
     1261 acacagacgt attgcagctt ctgccgcctt cgcctcgctg gtcagctcac agcgcgtcaa
     1321 cataacatcg ctggcaaact gaataaacgc gtcacagcgc tgctgttggt taagttgggt
     1381 aagccgctcg ttagcatagt tgttaagcgg cgcatcttct gcttgcggtt caagttgcag
     1441 cgggcgaccc agtgcctgag caacgttatt caaaaattcg cctcgattat ccattctttt
     1501 tctcctgcgc ctgatgtttc ttaaaccaac tacggaaact ctctccgtca gcttcaggaa
     1561 gatcgcgtgc ttccatccag tcgctaatcg cgccaaattt gagtggtgtt ttgccgccat
     1621 tgataaacca gcttgccgca tgagcgccgg ccatcatccc gactttccac aatcctggat
     1681 gactattggc ataagcgaac atttttatcg cccgttgctc tgcttttgcg gtgatccctt
     1741 tttcagccat cacccgacga tgacgcaaaa tcagttttga cagcggaata cgcaccggac
     1801 acacgctgtc acaagcagtg cataaagagc aggcgtaggg taaatcttta aaatctttat
     1861 agccgccaag tagcggagaa atcaccgcac caattggccc tggataaata gagccatatc
     1921 catgaccgcc aatatggcga tatgccggac aagtattcat acaagccccg cagcgaatac
     1981 agcgcagcac atcccgaaat tcagaggcca gcacctcaga acgcccgtta tcgacaataa
     2041 cagatgaaac tcttcaggac catcaacgtg ccccgcttcg cgcggtcctg tcagccaggt
     2101 gttgtatccc gtcaaacgtg caccaacggc actgcgcgcc agcatggtga tcaatacatc
     2161 tacctcggca aacgtggggg caatacgctc cattcccatc actgcaatat gcgttttagg
     2221 cagcgtggta cacattcgcg cattaccttc attggtcacc aggcataccg aacctgtctc
     2281 tgccaccgcg aaattacagc cggtaatacc tatttcagca ctgaggaaat cttcgcggat
     2341 tttttgccgg atgaataagg tcatcgcttc aggcgtttcc ggcccctcat agcccagacg
     2401 ttcatgtagc actcgacgga tctgatggcg atctttatga attgccggga ccacaacatg
     2461 agatggcgga tcttgatcca gctggagaat atattcaccc agatcggttt caatcacctg
     2521 aatgccagca tcctgcaaca catgattgac accaatctct tcggtcacca tcgatttaga
     2581 tttcaccacc ttccgggcat ttttgcgttg ggcaacctgt aaaatgtagc gggtagcgtc
     2641 ttctttggtt tttgcaaacc agacgtgacc gccgttttgc gtcacttttt ctgagagctg
     2701 gtacagataa gcgtcgagat tactcagaac atgatcacgt atctgggcgg cccgatcgcg
     2761 ccactcctcc cagtgcccca attcatcgac cattttttgc cgatttgccc caatacgctg
     2821 ctgcgcgttt gccaccgctt tgcgcatgat cggatcttca atttgctgac ggatgcgtgt
     2881 cttaaaatct gtattactgg ttttgatcga catctttata tcctcagcgg ctcatcaaca
     2941 cttcagcaat atgcatcact ttgactttct gcccttcccg ttgtaatcgc ccactgatgt
     3001 tcagcaggca actcacgtca gcaccaatta aatactcagg gcggacttcc atcaggtgca
     3061 caatcttttc tttcaccatc tcgccggata tttcggccat tttgaccgag aacgtgccgc
     3121 caaatccgca gcaggtatcc tgttcagcaa aggtcaacag ctccagtcca cgcacatttt
     3181 tcagcagcgt aagtggctcg tccttcactc ccagcttacg ggccaggcta caagatgggt
     3241 gatacacagc tctcccttgc aaactggcac ctacatcgac tacccctaat ttattaacaa
     3301 taaaagaggt gagatcctgc atacgcgcgg caaccttttc ggcacgtgat gcccattcag
     3361 gttcatccgc cagatacgtc gggtaacttt ttacggcata ggtgcaagag ccagccggtg
     3421 aaataatggg atcgtcgtta tcctccagtg cggcgatcag atttttcatc cctggaatcg
     3481 cttctttgat ataaccgcta ttgatcgcag gctgaccgca gcatccctgt ttctccggga
     3541 aatttacgcg acagccgagt ttttccagta gcagcacgga gtctcgtgcc attcttgatt
     3601 tcagggcgtc accaatacag gtgacaaaga aattgacatt cacaactaat actccattac
     3661 ttcatgccca tttatgcccg atgtttcccc cccaccgggg gatcaatcat caataaatat
     3721 ggcgttcttt tttaattggc tagcaacata ccacccctca tttgtatgac aaatgataat
     3781 ctttcatcca aacgtcaaat gcaataacta attttatttc tttcggcatc gctcacattt
     3841 tatggggaaa tcgacgatca tttttggcat aagaaatcca tttaaaaatg gctacatccc
     3901 ttatacctat tcagagctac ttattcaggg gttattgaga tatacactga tggtgtacaa
     3961 aaaaatcacc gacaataatc acgtatcaag taatagtcgg gattttattt aattgaagca
     4021 atttagtttg catttttatt taaaaactga gctagagtcc atcagaaaaa atatataaag
     4081 cccaaaccac cgttggaaat agaaatagtt gaaaggctca caactcccgg aatgcggatt
     4141 atctttgccc tgatttctgg cggttttatt cagggtgcaa gctgtctgac cctttcccga
     4201 tattctcccg gggtacaacc aaactcgcgg acaaacgcct tgtgaaaaga tgattcactg
     4261 gcatagccta ctgactcagc gatcaccaca acagggagcg tttcccggga aaacatctgg
     4321 gccgctattt gtagacgcaa ctttgttaat acagccagcg gcgtggttcc ggaaacatca
     4381 cggaaaagct gggcaaaact tgcccgggac atgtgagcga tgctggccag cgattcgacg
     4441 gtccaggcgt gtcctggcat ttccagcatt tgctgtatta ccgcaccaag acgtggatgc
     4501 agaagcaaac tgagaatgtt tttctcagta ttaacctgtg caatccactc acgcaccgcg
     4561 agggtaaaga atgtagcgca gatctggcta cacagtgcat ccactcccgg cattgccgat
     4621 ctggattcct gttgtaaaaa cgggatcgcc tccctcagcc agttatattc cacgctgtgg
     4681 ttaaccggtg ctaaaaacag cgtttccggc aaagacgtta aaaaataacg cgccgaatgt
     4741 tgcaaccgaa gagtgccgca gacaatacag gtcgattcat tatcgacatg actcaggcga
     4801 tgagctgaat tttgtggtag caggaccaca tttcccgggc gtaatgtaaa aatctccccc
     4861 gtcggcattt ccagcttcgc cgctccttgc gttaacgcat gccaacgaat aaccgataat
     4921 tccccggcac catgtggaag ctgccagtca cttcctaaca cgcaattctt atcgatcgtt
     4981 ccttgtggag cgttaagcat caacaaacgg ctaagggcat ccatatcaga ctcctgagtg
     5041 gtttttgatt acaaacacca acaatcttag taaataacta atctaatacc atcaaatagt
     5101 taatcatgat ttttgttttg tgattctgat taagaaaaag aaaatatcag acgataagac
     5161 aaaatatcaa gcgttgtgaa gaaatgttat ttgctctttt gcgtctatag tcatgatgtc
     5221 aaatgaacgc gtttcgacag gaaatcatca tgaataaata tcaggcagtg attattggtt
     5281 ttggcaaggc tggaaaaaca ttagccgtca cgctggcaaa agcaggttgg cgtgtggctc
     5341 tcatcgaaca atcaaatgca atgtatggcg ggacctgtat taatatcggc tgcatcccaa
     5401 ccaaaacatt ggttcatgac gcacagcagc acacagattt tgtccgagcc atacagcgta
     5461 aaaatgaagt ggttaatttt ttacgtaata agaattttca taatcttgcg gatatgccca
     5521 atatcgacgt gatcgacggc caggcggagt ttatcaataa tcatagcctg cgtgttcatc
     5581 ggcctgaggc aaatctggaa attcatggcg agaaaatttt tattaatacc ggtgcacaaa
     5641 ccgtggttcc gccaattcct ggaattacca ccacgccagg agtatatgac agcaccggat
     5701 tacttaatct aaaagaattg cctgggcatt taggtatttt gggcggcgga tatattggcg
     5761 ttgagttcgc ctctatgttc gctaattttg gcagcaaggt aaccatttta gaagcagctt
     5821 cgctgttttt gcctcgggaa gatcgggata ttgctgataa tatcgcgacg attttacgcg
     5881 atcagggcgt cgatattatc ctcaatgccc atgtggagcg aatcagtcac catgaaaatc
     5941 aagtgcaagt gcatagcgag cacgcccaac tggcggtgga tgcactgtta atagcttccg
     6001 gtcgtcaacc ggctaccgct tcgttacatc cagaaaatgc cggtatcgca gtaaacgagc
     6061 gcggggcaat tgtcgttgac aagcaattac ataccaccgc agacaatatt tgggcgatgg
     6121 gagatgttac cggcgggctg caatttactt atatttcact ggatgattac cgcattgtgc
     6181 gtgatgagtt gctgggagaa ggcagacgta gtactgatga tcggaaaaat gtgccttatt
     6241 ccgtattcat gacaccgcca ctgtccaggg ttggtatgac agaagaacag gccagagaga
     6301 gtggtgctga tattcaggtg gtgacattgc ctgtagctgc aattccgcgt gccagagtga
     6361 tgaatgatac tcgtggggta ttaaaagcga ttgttgataa taaaacccaa cgtatattag
     6421 gggcatcact gctgtgtgtt gactcccacg agatgatcaa tatagtgaaa atggtgatgg
     6481 atgccgggct gccttatagc atattacgcg atcagatatt tactcatccg tcgatgagcg
     6541 aatcactcaa tgatctattt tcattagtca aataaactca taatcagacg ccagaacaaa
     6601 tattctggcg cctcagagaa aagaatctta ttaattctct gttactctat aacttcttaa
     6661 tcacttcatt gatggtattt tatatgttta aaaaatctgt tttatttgca acactattat
     6721 ctggcgttat ggcattttcc accaatgcag atgataaaat aattctgaaa catatcagcg
     6781 tctcgtcagt atcagcatca ccgacagttc tggaggatac cattgctgat atagccagaa
     6841 aatataatgc ttcatcctgg aaagtcacat cgatgcgaat tgataataat tcaaccgcaa
     6901 cagcagtatt gtataaataa ggatgttcac aatggaaaaa tacctgcacc tgttaagtcg
     6961 gggagataaa attggcctga cattgattcg tctgagtatt gcaattgttt ttatgtggat
     7021 tgggttatta aagtttgtcc cttacgaggc agacagcatt acaccattcg tcgcaaacag
     7081 tccactaatg tcgttctttt atgaacaccc ggaagactat aaacagtatc tgactcacga
     7141 aggcgaatac aaaccagaag caagggcatg gaaaacggcc aataatacct atggtttttc
     7201 caacggtctt ggcgtcgtgg aggtgattat tgctctgctg gttttggcta atcctgtcaa
     7261 tcgctggtta ggtttattgg gaggactgat ggcatttacc acgccgctgg taacactctc
     7321 atttttaatc accaccccgg aggtatgggt acccgcattg ggtgacgctc atcatggttt
     7381 cccttattta tccggtgctg gtcgcctggt attgaaagat actctgatgc tggcaggtgc
     7441 tgtcatgata atggcagatt cggcgcggga aattcttaaa caacgcagta atgaatccag
     7501 ttcaacgtta aaaactgaat attgataatc cgaacactgt ctgttgttca ctttttcagg
     7561 cgtcaggccc cctttctgat gatgaagcct gacgccatat taccggagat atgacagagt
     7621 gaagatcaat atcactcccc tgttaaaaga ttcttgtcgt ctcaggtaaa atatatgatt
     7681 aatatcaatc atgtattttt acagcaagac aaccacgaac aaattcagga ttaaaatgtt
     7741 ttaccaactc cccaacataa cccaaatcaa gcgaactaat ttgtgccatt tctttctctg
     7801 tcagtgagaa atcccagata gcaaaatttt cttcgatacg ctgttgatgt gtcgatttcg
     7861 gaataacgac cactccccgt tgaatattcc aacgcagaat gacttgagaa atcgattttt
     7921 gatgggcatc tgcaatactc tgaagcagat tattttcaaa gggtttatgt cgtccaccac
     7981 ctaacggagc ccatgcttca ggctgcacgt tataatactt catggttgcc agcgcctctg
     8041 gttgggcaaa atagggatgc aactcgacct ggttgaccat cggtttaact cttaccgttt
     8101 cacaaaagtt cgccagaaca tgaggataga aattggaaac cccaattgct tttaatttgc
     8161 cctcttcata ggcatcttcg agtgcacgcc acgcactgaa ataatcgcgc atagcctggt
     8221 gcaataaata gaggtcgaag tactctagtc ccgatttttt taatgatgct tcaatacctg
     8281 ctgctgccgt atcctgattc aacatatcct gcacccacaa tttagacgtt ataaataact
     8341 catctcgggt acataaacct tcagatatag cttcacgaac ggcttcaccg acggcatctt
     8401 cattaccgta taccgctgca gtgtcgatga gtcgataacc tgtgcggata gcgttcaaca
     8461 ctgactgttt gcacacgttt ttatcggtaa cctgaaaaac accaaatccc atcatcggca
     8521 ttttcagatt gttacttaat acggaaaatt ccactgtcag tccctccatt aggcatgaac
     8581 aatgagtcta cgttaaaacg taacctcaaa ttagtacgtg gattttgata tcacttatgc
     8641 aaaaaattca ttaataatgc aggactgaaa cctctctatt ttcggggaca acgaagcaga
     8701 cgctaccagt gcttttgcct tcgcccttgc tgtttttgat acacttaggg cccagggcat
     8761 aacgaaaatg tgcgatatga caaagaatta acggagaatg tgatgatcag gcagaagatt
     8821 ctacagcagc tcctggagtg gattgagtgc aatcttgagc accctatttc aatcgaagat
     8881 atcgcacaga aatctggcta cagcagacgc aacatccagc ttctgttccg aaatttcatg
     8941 catgtgcctt tgggagaata cattcgcaaa cgaaggcttt gccgtgccgc tattcttgtc
     9001 cggctcaccg cgaaatctat gcttgatatt gcactctctt tgcattttga ttcacagcag
     9061 tcattcagcc gcgaatttaa aaagttattc ggctgctctc cccgtgaata ccgccaccgt
     9121 gattattggg atctcgcaaa tatcttccct tcttttttaa tacgtcaaca gcaaaagacg
     9181 gagtgtagat tagtcaattt tcctgagaca cctatttttg gcaactcatt taaatatgac
     9241 attgaagtgt cgaataaatc accggatgaa gaagtcaaac tgcgacgtca tcatttagcc
     9301 agatgtatga ataattttaa gacggatatc tatttcgtct ccacgtttga accgtcaaca
     9361 aaatcggtcg atttgctcac ggttgaaact tttgccggta cggtatgcaa acaaaccgat
     9421 tcagagatgc cgaaagagtg gacaattaac cgaggactgt atgcctcttt ccgctatgaa
     9481 ggtgagtggg agcactaccc tgaatgggcg cgaaacctct atctgatgga attacccgcc
     9541 aaggggttag ccagagtgaa cggcagtgat attgagcggt tttattacaa tgaagatttc
     9601 gtagaaaagg atagcaatga tgttgtttgc gaaattttta tccccgttcg tccggtttag
     9661 ttttcgctat atctaaatga atttataacg agactactgt caggaaattc aataaataat
     9721 ggcgttgaag atgtcaacgc cattattgtt tattagaatg ttactttcat attcttaaaa
     9781 ttatttcatc gcctcctcgt cctttgccgt atcggcatcc accgcggtaa tggtcaccgt
     9841 cactggcttc atttcgtacc cgccataggt cagggcgtta aatgtgaacg tatagacacc
     9901 aggcttgctg gtactaaatt tacgcgtgat ttttccgctc acgaaatggt catttttcga
     9961 cggcaggaac tgataatcct tctccgtcac cccttccggc gtctgaatgt cgacccacat
    10021 attcccgcca acggggttgt ttgccgacat cagtgtcacc tccagcttcg cagtcgattt
    10081 cccatccgcc accagagatt gcatatcaga tgatgcggtg agtaccgcgt ttttatcatc
    10141 cgcaacgaat ttagattcgg cagttttcgt tgatacctga ccgttggtct gactcacttg
    10201 cgccttgagt gtgtatgtcg ccgcaacagt agtggtagcg gtgaaaatag cctgcccttg
    10261 ctcattcgtt gtcgcagtgc cattcggttc caggtttaac gaagccgggc tggcagtcag
    10321 cgtcacttcg ctatctttca ccaaattgtt attggcatca gtcaccgtaa ctttatacgt
    10381 ttgtttggct ttgccgtccg ccagcgcata gttgcccatc acctcaatac cggagacggt
    10441 tgcggtagcc ttatcggcta caaacgttat agtctgcgta ttcgaaggct ggctattccc
    10501 tgccaatgcc gtgatctcat acgttccggc agtcactgaa accacctgca actcggcttt
    10561 cccctcatcg ttggctttca cccagacatt atcgcctgta agcggcttga caccccgggg
    10621 cagattaaag gtcaccaggc tccctggaac agggttacca tttttatcct caacggtcgc
    10681 cgtcagcgta aaggcatctt tgccgtttgc cactttttga gcaggggcgt ctacctgcag
    10741 gtttgcctga ccggtgctgg catccgcgtt gaatttcacc gtgaccgtct tctgagcacc
    10801 attcaccgaa gcggaaatat tgtgttctcc cgcaaccgtt gacataagct caatgtccac
    10861 tttccctgcc gcattggttg ttactgtatt ccccgtcttg ctggtcacac cctgcggtaa
    10921 agtcagcgta acttcttgcc cctgcaacgg attaccatag ctgtccacaa cggtcagggt
    10981 tatctggtta gcagactgtc cattagccag ttggttatta accttcactg tcatatcacg
    11041 aatctcagcc ttagatgcgt cacctgcaac attcagcacc agtggctgag caacggcatt
    11101 ttggccgttc actcgcggca tcacagacaa ctgacccgcg gcagatccca gcgttaaggt
    11161 cgacacgtag accccattac ctttctctgt ccagtttcct gctgaaggac gctcactccc
    11221 cgtgctggcg gcaccgctaa acactggtgc atctggcttc agcccggtga ccgggttccc
    11281 gtacgcatcc ttcacagtga aggtgagttc cgtcgtcgtt ttgacggtcg gagccttatt
    11341 gtccgcaaca agcgtagagt tcgctgatga catctctccg gcgatgaccg tcaactgcgc
    11401 ggcttcggtg gctgctggct ggccgttgaa tagcggcatg acgagaagct cacccgtctt
    11461 tccacctgtt gtcagcgtag cgacatagga accatcacct ttttcggtcc aaccagaaac
    11521 agtcgcccct tcagacgcag cacccgtcag gcttgccgac agggaaagac cactgatagc
    11581 gttgccatgt gcatcttttg caataagcgt cacggttgtg ctttcgcctg cttttacgcg
    11641 atctgcggct acagacactg tcgactggcc tgaagataac gcatcagcca ccacggttac
    11701 tttagccgca ttcgctgccg catcctgtcc atttagcttc ggcataacct ctaattcacc
    11761 cgccgtagtg ccgagagaaa tctgcgcagt ccaggtcccg tcgccattat ccgtccagcc
    11821 tgatgccgta gaacctacag cagctgcgcc cgataatgac ggtgcatccg gattgagacc
    11881 agttacaggg ttgtcattag catcttttgc cgtccagatt gccgtaactg taccgccaac
    11941 caccggttta tcaggattca gggtgatgga cgaatgtgct gcatcaagcg gcccggcaac
    12001 aaacttcagc gtttgttgta atgtgcccag ctgatactgc tcgctgaaag cacgcacaac
    12061 aacgtttccg gcacgggtcg aagaaaccgt ggcgctgtaa acgcctggtt ttatttccga
    12121 aatggcacca acggttacac cattagtgtc ttgcggaaca aatcgcaagc ggctggcttc
    12181 tcccgtcacc ggattaccct cggagtccac cgcagtcaac gtcaacgtat aggcttgctg
    12241 accatcagca accacgtcac ctgacggctc gttagcgctc agggtggagt ttgccacatc
    12301 catcatcgtg gcccgcagtt ctgcagtgac ggttttgctc atgccatcaa cgctaacagt
    12361 aatcgttgcc tcacctgact gcgttccggt agtaaagaca gactgataca ccccggcttc
    12421 agtttcggtg aactcaccca gtgttggctt tgcctgtgat ttagtggcct tcagggtacg
    12481 agtcacaata tttccagccg gtttgaaagt tagttcagtc ttgatctgat ctttcatgcc
    12541 cgtgactggc tggccctcgg cgtcgcgcag agacagcacc agcggttttt gctcattacc
    12601 gttagcaagc atttgaatac ggctctgacc gtcaagcgtt aacgccgtgc gatcggcgct
    12661 cataccagct ccggtaatga ccacctctgt ctgcacgcgt tttgaggtat tgcctttgtt
    12721 atcgtaggca accgcagaaa tcgcataata attgtctttg cctggacgat aagccgggag
    12781 cgttacttgc cactgactac cctgaccggt aattttgcca ccttcagcca gtaatgacgg
    12841 cgcttcccac tgcacatttt tcagtccgtg agttgctttg ctgaccacaa gcccgaggga
    12901 aagtgtctga ccgcccttgc cttcaatacg ctcaggcaga gcaatacgga tcacttcaga
    12961 tttgcggtac tcaagaacga tgttgttatt acgctcaacc aggtcatagc ggctgcctgc
    13021 cagcatgcga cgctcgcgaa tgctgtctgt atcgagttgt ttctccagag gttcgccaat
    13081 ccggtagtta acttccaggc caaagcgagt gtcattctca ccgctcttgc cctgcttatg
    13141 cccggcgctc agtgtcagaa gaggcactgg cgtataggtc acctcggcag aaatagcatg
    13201 cgggtctttc tggcgcttat ctttaccaaa cagcccgact tcatcgccat aatactgttc
    13261 atacatcagg cttgcgccaa gctgcggcca ggcaggtaaa tagccctctg cgcggatatc
    13321 ccaaccattc gccgggcgtt cctgataatc ctcaatatcc ggcgattttt tccagccaga
    13381 agcccgaata taaccattgg cgctcagttt cagataatcg cgccagtatt ccgcaccaac
    13441 accaatgcgg gtatgactac gggataaatc atgatcgata aaagtattca cccccgccat
    13501 ccagtcattt cctgaaaaat gacgccagcc aaaaccaata tttgactgag tacgatcgtc
    13561 tgtacgatgt attgccccct gagtgaacaa catatttgtc ggcgtatcat aaatcggata
    13621 aagcatttcc agcgaagaat ccttcagcga gaaatcttta tcgacattca gtttgacgcg
    13681 cgctgtacca tatttcccga gccactcctg tatttcctgg ttagctttag cggtggccat
    13741 tccggtaata aaattacgtg tcgcatcgct atctgtctga ctgcttaaaa atgtcccggc
    13801 atttgcggca aacgacgcga catttttctc cacattatta tcagcagtta ccgtggtatt
    13861 ttccatgctc aaccgtggct gaaccgcatg ctgcgcatgt gccgccatta ctggggtaaa
    13921 ggtgacagcg agtggaaaaa gaacctgaac agagatattt gcccacgcca cgcagcgggc
    13981 cagaactgaa taacgaaata gtggttgttt atgacctgtt ttataacgtg acattgagat
    14041 aatttcctga tgaacgaatt aacgaacata ggcagacaca aataatggcg tctgcttgtc
    14101 agaaaaaccg gagatttccc ggtttccttt tccgcagaaa aggaaatacc tttatgtaag
    14161 aaagtacacc gccacctaat atatccagtg cctgtagcca ctcatcaggt ggttgggcgc
    14221 gggattatat ttttattt
//
LOCUS       DAZKIV010000120        14235 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZKIV010000120 DAZKIV010000000
VERSION     DAZKIV010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.120"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(70..489)
                     /locus_tag="ACHOT6_003492"
     CDS             complement(70..489)
                     /locus_tag="ACHOT6_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9948652.1"
                     /translation="MKTRFYFCSEDTGFYILPGSSFVPKSSIEITAEQYAKYAGVAWP
                     ENKVLGVCDDGNLAWLDAPQLSYEEKLIKNESQKQKLISIANEYINSKQWPGKAAIGR
                     LKSEELVQYHLWLDYLDEVIAIDTSSETDIIWPKPPK"
     gene            complement(491..1912)
                     /locus_tag="ACHOT6_003493"
     CDS             complement(491..1912)
                     /locus_tag="ACHOT6_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9948653.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDEVLELK
                     LYVDDQMRNHIAAQDPHTQYAQKHNPTFTGEPKAPTPAAGNNTTRIATTEFVQAAITA
                     LINGAPATLDTLKEIAAAINNDPKFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAAMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLAYLGLGEAAKMRASNTVASTSGRISIPTFVEGVERELIIQWVTGV
                     ISDGNGVAKITLPMAFSNALMYAVAVDCGASESAWPGNSGTTWGGDFYNSSRTTVIAR
                     VLKFANGNWYGGTAAGSILAIGY"
     gene            complement(1909..2514)
                     /locus_tag="ACHOT6_003494"
     CDS             complement(1909..2514)
                     /locus_tag="ACHOT6_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFP9948654.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTAEAPGTFRLDIGVKDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            complement(2507..3415)
                     /locus_tag="ACHOT6_003495"
     CDS             complement(2507..3415)
                     /locus_tag="ACHOT6_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFP9948655.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGSDLDQLAANYNVKRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            complement(3402..3761)
                     /locus_tag="ACHOT6_003496"
     CDS             complement(3402..3761)
                     /locus_tag="ACHOT6_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFP9948656.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSSLIDQPQNPALRLQVMSAVYVALSRWEPRMTLDSITINSNFDGSMVVALSGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            complement(3758..4336)
                     /locus_tag="ACHOT6_003497"
     CDS             complement(3758..4336)
                     /locus_tag="ACHOT6_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFP9948657.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGELETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAIYSNQFAPPSTSANACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDTHQHTGVLKGGDTTGGPV"
     gene            complement(4405..4851)
                     /locus_tag="ACHOT6_003498"
     CDS             complement(4405..4851)
                     /locus_tag="ACHOT6_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFP9948658.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSRMMRQLAQELHRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTAASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            complement(4844..5275)
                     /locus_tag="ACHOT6_003499"
     CDS             complement(4844..5275)
                     /locus_tag="ACHOT6_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9948659.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNAVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPTLREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAVAEPDEPEEMWTVKRG"
     gene            complement(5238..5486)
                     /gene="lysC"
                     /locus_tag="ACHOT6_003500"
     CDS             complement(5238..5486)
                     /gene="lysC"
                     /locus_tag="ACHOT6_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461641.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFP9948660.1"
                     /translation="MLTLSGCGNVRRSPEVQLTVSGCPRVTPCRLDQAAPRTNGDLNA
                     LLDETEAAWAVCADKVDMIIACQERNSEQTTIPAPRPQ"
     gene            complement(5371..5799)
                     /gene="lysB"
                     /locus_tag="ACHOT6_003501"
     CDS             complement(5371..5799)
                     /gene="lysB"
                     /locus_tag="ACHOT6_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFP9948661.1"
                     /translation="MNRLLLVVLALLLASLGWQTWRLADASQTISTQADELRSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTNALLRQRQHRIEELKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGSGSAAH"
     gene            complement(5796..6173)
                     /locus_tag="ACHOT6_003502"
     CDS             complement(5796..6173)
                     /locus_tag="ACHOT6_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFP9948662.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WGAIPVALVALIKNGPLWQELRASLLKSITRKENVFISWVMRLLIIVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            complement(6175..6687)
                     /locus_tag="ACHOT6_003503"
     CDS             complement(6175..6687)
                     /locus_tag="ACHOT6_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9948663.1"
                     /translation="MNPSIVKRCLVGAVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDATVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            complement(6668..6883)
                     /locus_tag="ACHOT6_003504"
     CDS             complement(6668..6883)
                     /locus_tag="ACHOT6_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HP1 family phage holin"
                     /protein_id="HFP9948664.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            complement(6887..7090)
                     /locus_tag="ACHOT6_003505"
     CDS             complement(6887..7090)
                     /locus_tag="ACHOT6_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFP9948665.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            complement(7090..7554)
                     /locus_tag="ACHOT6_003506"
     CDS             complement(7090..7554)
                     /locus_tag="ACHOT6_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFP9948666.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYVSLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAESSGDLWRDARWAISRVQDAPHCTVELI"
     gene            complement(7650..8300)
                     /locus_tag="ACHOT6_003507"
     CDS             complement(7650..8300)
                     /locus_tag="ACHOT6_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFP9948667.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLTAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLAATGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            complement(8304..9362)
                     /locus_tag="ACHOT6_003508"
     CDS             complement(8304..9362)
                     /locus_tag="ACHOT6_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFP9948668.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGLGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRNAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETIPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAEAGA"
     gene            complement(9379..10212)
                     /locus_tag="ACHOT6_003509"
     CDS             complement(9379..10212)
                     /locus_tag="ACHOT6_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFP9948669.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAYNPLANRKQNPANLFT
                     AAEETVSELEEIQEDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERMSELETALQEQQTAFNELVNKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            10355..12121
                     /locus_tag="ACHOT6_003510"
     CDS             10355..12121
                     /locus_tag="ACHOT6_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFP9948670.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAALYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKGPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRSTTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            12121..13152
                     /locus_tag="ACHOT6_003511"
     CDS             12121..13152
                     /locus_tag="ACHOT6_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9948671.1"
                     /translation="MGKSKKNRAAATNQIQLKSQTTAEAFSFGDPVPVLDRRELLDYV
                     ECVQMDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKHTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASKVFVRNELISMQKRIKEINVWLGTDII
                     AFTEYNLDM"
     gene            complement(13202..13423)
                     /locus_tag="ACHOT6_003512"
     CDS             complement(13202..13423)
                     /locus_tag="ACHOT6_003512"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948672.1"
                     /translation="MEDLFDSTVLSTVLDGKTFNKSNDTDTKTEYGKHVFSTKVIKAN
                     CKTISFEKFKVIFDGVEEIIADYSKRCKV"
     gene            complement(13456..>14235)
                     /locus_tag="ACHOT6_003513"
     CDS             complement(13456..>14235)
                     /locus_tag="ACHOT6_003513"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HFP9948673.1"
                     /translation="NDKKTRVCYKTSRQDVTGLTVNKKVNVGKEYAKVTRAMAHSLYK
                     DGSFVIVTESGKIEKGTCAKLEGRFGFIDSIDKYNNLKLKNARQLEKYQSVNHGLNYT
                     AKLNSREKAYSYFLYYKNFHGMEMPTILTEGKTDRVYLKCALKSLANSYPLLFNSSDD
                     SSKNEYRVNFFKATEKTSYFLDISGGATDFKRFILRYQEQKKRFEKFKPKHPVIMLLD
                     NDSGPKDLLNHLKDKVKIVQMMLILFVRQGIPTYLTISICY"
BASE COUNT         3215 a         4040 c         3502 g         3478 t
ORIGIN      
        1 gcaaacgttc gaggcaatat aaaaatcgat aaattaacat atataaacca ttttgttaat
       61 gaataataat tattttggcg gttttggcca aataatatcc gtctcactgg aagtatctat
      121 cgcaattacc tcatcgagat aatctaacca taaatggtac tgcactagct cttcgctttt
      181 cagacgacca atggctgctt tacctggcca ctgtttactg tttatatact cattagcaat
      241 agagattaat ttttgttttt gagattcatt ttttattaat ttttcctcat acgatagctg
      301 gggggcatca agccatgcca gattcccatc atcacaaact cctagtactt tattttctgg
      361 ccacgcaacg cccgcatatt ttgcgtactg ttctgctgta atttcaatag atgacttagg
      421 tacaaaagaa ctaccaggta aaatataaaa accagtatct tcactgcaaa aataaaatcg
      481 agttttcata tcagtatcct attgcaagta tacttcctgc tgctgtccca ccataccaat
      541 ttccattcgc aaattttaat actcgtgcaa ttacagttgt tcttgacgaa ttataaaaat
      601 caccccccca cgttgttcca gaattaccag gccatgctga ttcactggcc ccacaatcaa
      661 cagctactgc atacattaat gcattagaga atgccattgg cagtgttatt ttcgctactc
      721 cgttaccatc agagatgacc ccagttaccc actgaataat taactccctc tctactccct
      781 caacgaaggt tggaatcgaa attcgccctg atgtggaggc cactgtatta gatgccctca
      841 ttttcgccgc ttctcccaaa ccaaggtatg cgagaagacc agctacatcc tttccactca
      901 aattggtaag cgtattgtcc agcggttgtt tacctgccag cgcattaagc attgtcgtgg
      961 caaagttcgg atcattcccc agtgccgccg ccagttcgtt cagtgtatcc agtgccgcag
     1021 gtgcagaccc caccattgct gcaatcgctg atttcacaaa agctgtagtg gcaatctgtg
     1081 tattgttgac cgactgtgcc gcagtaggtg ctgttggggt tccggtgagt gccggactcg
     1141 acagcggtgc ttttagtgcc agcgcattgt taatggtggt gctgaatttc gggtcattgt
     1201 taatggctgc ggcaatttct ttcagtgtgt ccagcgtggc tggcgcaccg ttaatcagag
     1261 cagtaatagc ggcctgaaca aactcagtgg tcgcaatccg cgtggtgtta tttcctgctg
     1321 caggcgtcgg cgcttttggt tctccggtaa atgtcggatt atgtttctgc gcatactggg
     1381 tatgaggatc ttgtgcggca atgtggtttc tcatctggtc atccacatac agctttaatt
     1441 ccaggacttc atcatccacg tatttacggg ttgccagcac tacagcaggg tcgattttca
     1501 gggtgatatt gtccgtactg ctggtaatca gcaccatgcg cacggtctgg gtgcgcccgc
     1561 tgccttcagc cagttgcggc ttatagcttt ccgggcagtt gcccacggca atcaatgccc
     1621 cggactcatc aaacaggccc acttcacgta tccaccaacc accctcgttt tcagggatca
     1681 cctgttcagc aataatctgg ctgctgttct gcgggtcgat ataaagcata ttcagcgcag
     1741 cccggcgttt ctcatttacc agtgccgtct gctttgcgtc cggcgttggc aatactccgc
     1801 cgccatcgcc caccgccata tgggtaattt ttagcggcac accgagcgcg gcggcgctgg
     1861 caagtttcgc cgcgccaata tccgtcagca gggtataaaa ttttgtgctc atggattcac
     1921 tctcattgtg tcaataacat ggaccgcccc gccttcatgc gcggtgccac cggaaataat
     1981 cgtttcgttg atatacggat agatcgtgat ttcttcgcca agatagctgg cggctcccac
     2041 ccaatgcggg ccgctggtct gcagattgat ggacatgccg atcatgtggc ggctacatgg
     2101 tttggcatcg cttatcagtc gctcaagttc cagataggta tcttcagtga tgccctggtc
     2161 cttcacgccg atatccaggc gaaacgtgcc cggtgcctct gcggtctgcc accactcaat
     2221 aatgcggatc agaaagccga acggctccac cacccgccgc acggcactgg tggtcccttt
     2281 atgctgatga atataaaaag catccttcac cacctggcgc ttgacgcttt ctgtccagcc
     2341 ctcgtcccag cgatccacag agaacgccca ggcgagataa ggcaggaaac tgaccggaca
     2401 ggttgccgga ttccacaagt cacgaagcgg cacctgcaga tcagaaatcc cgctgcaggt
     2461 ttgcgccagt cggcgttcca gtggtgttga acccggtggc agcagactat tcatccgttc
     2521 ctccgttggt tacgctccac tgcgtacatg atgccgcctg tgttttgttc aggaccacat
     2581 ccgccagagg agaagccagc tccacacgct gcacaccctc aacatgcagg gcggcaaaga
     2641 tggcgctacg gcgaatatcc cgaccaagac gcgtctgact ggcgatgtac ttctgcaggc
     2701 tggcttttgc cgctgccatt accggctctg cttccggtcc aggatagaga aaaatggtgg
     2761 cttccacacg atacgggatg atttctgcgc tgcgaaccgt aagacggtca gccaccgggc
     2821 ggacgttctc actgttcaga gctttttcca ccacgtccag caggtctttt tctgcagttc
     2881 catcgccttc gcggctaagg acagtcagca ccacctctgc aggtgctggg ctggttgcac
     2941 tggcatccgc cacccgaccg tcggcgcttc gggcatgaaa ttcataagct gcagttggcc
     3001 ccgcaactga aagcccttca aaggctgcag gcacacgcag gcgtaacgct tcatcgcttt
     3061 ccatcacagc tgcaacgggc ggcacagcgt cattatcagc aggcgtcacc gtcaggcgtt
     3121 tcacgttgta gttggcagcg agctggtcaa gatcgctgcc catcgcgtaa gccaccatca
     3181 cagcctgcgc ggcttcgtta atgcgctggc gcagaagcaa ctcacggtaa gcgttctcct
     3241 gcagcaattt ggtgacgggt tcagattcca gttccagcgt gcggatcacg gcttcctgct
     3301 catctttcgg atgaagcgca acaaattcgg ccttgcgttc ggcaagcagc gtctcaaagt
     3361 ccggcacatc cacaatctgc ggcgcaggca actgcgaaag gtcaatcact gccattctct
     3421 gctcctgttg atacggaaag ggaaacaggc acaccgttat tacgccgccc gctcagcgcc
     3481 accaccattg aaccgtcaaa attgctgttg atggtgatgg aatccagcgt catccgtggt
     3541 tcccagcgac tcagcgccac atacactgcc gacatgacct gcaggcgtaa tgccggattt
     3601 tgtggctggt ctattagcga cgacagcagg gaaccatatt cacgacgagc aatgcggcta
     3661 ccctgcggcg tcagcagaat gtcccgcacc gactggcgca gatggtcaat atcagtaatg
     3721 actttgccgc tggtattgtt catcccacta taaagcgtca taccgggcct ccggttgtat
     3781 cgccgccttt caggacgcca gtatgctgat gcgtatcaac cacgatcccg ttagaactca
     3841 tcgctccgcc gccctgggta acgccaccat tgatcaccac ttcgctgtta atgcgcgtgc
     3901 ggtcagcctc cagtacaaac tcactggttt tcatggtgat gttgtcagcg gcctcaatga
     3961 ccattgattt gatgcccctg acataccacc gcccggtggc gggttcgtat tcaaaccagc
     4021 caccgtcagg atgttctgtc acgcaggcgt tcgccgacgt cgacggtggc gcgaactgat
     4081 tcgaatagat ggcgggtaac gcaaaggcgg tttccagatt gccgcccaga ctcagcagca
     4141 ccacctgctc accttctgat ggtcgccacc atgtgcgggc attcccggca cgcagcgtca
     4201 gccagctgat ccagttggtt tcaagctcgc ctgttttcac ccgacaaagc cagttttccc
     4261 ggtccacttc ggtgactaca cctgtgcgga tcagattggt gataaggcgc atgatttcgg
     4321 ttaattgtgc gttcataggg aaaggttgcc atcaggggaa gaaaggcggc agtgctgcaa
     4381 cttgtatcag tgctgataca aagatcaccc cgccagccat tgtagaatca tgtcgcgggt
     4441 cattgcctca acatcatcat ttacacccag caggcggcgc tctgcgtaac ggacctccgg
     4501 tcctttgcga ctgacgcgat cacgcagacc gtaatggtga acacgggcaa tacgctgcac
     4561 cttgccttca aactgtacgc tggcagagtc ggcgctggcg gcagttttca ggtattttgt
     4621 ggtgcgcagc tttgcaaaca tctgacgttt gatgcgcccc ttcttgctgc gtgctgttac
     4681 cctgcgcggt tcataactgc taccatctgg attgcgctgc atcctgatat tctgctgctg
     4741 tgtccggtgc agttcctgcg ccagctggcg catcattcgg cttcttgcgg ctggttccag
     4801 attcgccagt aaagcactca gccagtcgtc caccttctgc agttcagcca cgtttcaccg
     4861 tccacatttc ttcaggttca tcgggttccg ctaccgcttc aacgctcgac acactgccgt
     4921 cagtgctgac cagcacacgc tccgtcagct gcaggttcag gctgatatca cagacatcgt
     4981 tgcgcagaat atccacctca aaggtgaata gcttttcccg taacgtcggg ttattgatgg
     5041 catcaggctg gttatcccgc agccacagca aaaccggggc catcagcaga ttctggtcgc
     5101 cgctgaaatc ttcaatcacc gcgttcaggg tgtaacggta ctcccacgac atggagctgg
     5161 cccccgtggc aaccagcgaa ccgttatcca caaacagatg cagtttgtcc gggttattgc
     5221 ggacataagg caccgcttta ttgagggcgt ggcgcaggga ttgtggtttg ttcactgttt
     5281 cgctcctgac acgcaataat catgtccact ttgtctgcac agaccgccca ggcggcctcc
     5341 gtttcatcca gcaacgcgtt cagatcaccg ttagtgcgcg gcgctgcctg atccagccga
     5401 cacggcgtca ctcgcggaca accactgacg gtaagctgca cctccggtga gcgtcggacg
     5461 ttcccgcagc cggataatgt cagcaggcaa aggagtatca gcccagcggc gtaaatcctc
     5521 gttctcacgt ttcagttcct caatccgatg ttgtcgttgt ctcagcagcg cgttggtctg
     5581 ttctgcttcg gcatagagcc gcgcctgctc ccggttattg gtttcagtca gaatggacag
     5641 gctgataagc tggctgttgc tctttgccag tgcctggctt ttgctccgca gctcgtctgc
     5701 ctgcgtgctg atggtctggc tggcatcagc cagccgccac gtctgccagc ccagcgacgc
     5761 cagtaataac gccagcacaa ccagcagcaa ccggttcatg ctgctacctg ttgcgccatc
     5821 tggttacgag tgatccagaa ggcaataacg gtcagcagat aaaagaccag ggtaatggcc
     5881 caccccgtcc aggcgagact tacgataatc agcaatcgca taacccagct gataaatacg
     5941 ttttcttttc gggtaatgct cttcagcaaa gatgccctca actcctgcca gagcgggccg
     6001 ttcttaatta acgcaaccag tgctaccgga attgcccccc atgtcagcag gcaggctacc
     6061 cagacaccgg acgctgccag taccggaaaa atcccctgcg gatacaccat tgctgcgatt
     6121 aacagcgcca tccataacat cagaaacagt ccgctgatta atttcttttt catttcagtt
     6181 tgctccctgt aaacaccagg ccatctcccg cgcacggcgg ttatccagcc cctgattaaa
     6241 cacacctttt acataaaccc agcgcggcaa ctgtcggcac gcatccgccc agcgccgctg
     6301 attgagcaat ttcaccagcg tggagctgca ggcattgccc gtccccacgt tgaaggcaaa
     6361 cgacaccgta gcgtcataca ccttctgtgg tggctgttgc ttcacacacc tttccagtgc
     6421 ccgctccaca cgcagcacgt tggagatcag cccttctgct gcctgtcgtt ccgtgattgt
     6481 tttgccggga atgacgcccg atgtattacc aatgccgtcg gtccagacac ccgcgctgca
     6541 ctgatacggc tgcagacgac agccttcgta atcggcaatc agtttcagcc cctccacgga
     6601 ggtgtgaagc tgctgaaaac ccggcagcgt ggcagcaata gccagcacag ccccgacaag
     6661 gcagcgttta acgattgatg gattcatagt cctcccgcga gatctgcccg tcgcgcagaa
     6721 gctggtaggc tttgtgtttg tagtaccagt tgatagccag catcagcaca ccgatcatca
     6781 ggccgcccag cgttgaggca tccttgatgg acaaatcgcc cagccaggcc agcacgacgg
     6841 cgatgcaata cgtgataaag gcgctgattc gctcaagcgt cataattcag tcccatagct
     6901 ggacggtctg cacggtggtg gttgtcggaa tgtccggcag ctccacctgc agcccgtgag
     6961 gtaaaaaggg gccgtattcg gcaagccccg gatttgcctt cagtacctgc tccgtgacac
     7021 cctgcgtgcg cccgtaatga cgccagcaaa gcgcgtccac cgtgtcatac tgatgcgcac
     7081 gcactttcat cagataagct ccactgtgca gtgcggcgca tcctgcaccc gactgatggc
     7141 ccagcgggcg tcacgccaca aatcaccgct ggattccgcc agttcctcgc cccgcttcac
     7201 accggatgcc gtggcgtcat agtcttggta tcgttcgttg agcatggcgc gtgcccagca
     7261 gtaaaccgcg ttgaaatagt gctgaatgcg ctcacttttg ccgtccagct gttccgccgg
     7321 aacctcagcc agcgacacat atcccagcat ctgctgacgt ctacgaaact catacagctc
     7381 tgcgttgacc tccgaaattg ccgacagggc aacctgcttt aaacgcggct gcgtcaccgt
     7441 gccgtcagtg cgcatcacgc tgcgaaactc cgacaggtcc acatcaggcc agaacggcgt
     7501 atttctgatg atttccgcct gttccggtgc ctgttctggc gcaacaaact tcatgctgct
     7561 ttctcctgaa atagagggcg gtggacggga ttttgatgtg gctgtgcctt tcgccacccc
     7621 gtgccgcccg tgcgcggggg cacgttctgt cagcggctgt cattgcgcag tctgcgctcc
     7681 agctgctgtt tgtctttttt cacgccacag cggggatcga gctgtaacgc atggttgaga
     7741 tggttaaggg cagaagccgg attgctttca ctcaggacag cgccaatcgc tttatgcaga
     7801 cgcgcccgtg actggtccgg catatccaga ccgtctgtca gctccagcgt ctgcaacaac
     7861 agatcggcat caaagccggt ggcggcaagc attgcgctct gcgccgcgtc tgccatttcc
     7921 tctgccagca cggtctgcac attgcggtta cccagcggca tcacccagcc atgacgcagg
     7981 gcatgacgcc cgatctccaa cgccccggca taatctccgg catcaatgcg ccacagcatc
     8041 acgtacatca gcacgtcatc ctgctgtgcg cctccggcag tcaggacacc ctctgcccag
     8101 gcggcgtact tcggcagcag ctccaccttg atttccgctt ttttgaccgt ggactgaacg
     8161 cccttgagac ggcggcggtc ttccgccagt tgcagcagca tcaggtcata gcccgacgcg
     8221 tggcgaacac tgccgccctc gcgggcggcc tgttcagcct gaacgcgcag gcgatgctgc
     8281 cgtgcgggac tcaggctcat ggtttatgct ccggcttctg cggcggcggc gctgaagtcg
     8341 ccaatctgga tgttttccac cagtgctgcg cagcggtagt cctcaaccac ataggcttcg
     8401 ttaacggatt caaagttttc aatccggtca cgtttcgggt tgtcgataac cgaacggcgg
     8461 cgggtgtcct cctgccagta aatggacagg ttatccagac gggtgatcag cagcgcattc
     8521 ggcgggaaga acggcgcacg cacggcctgc agacccccca tgcgtttctg actgatgatc
     8581 atatcggcag ccagtttttc actgttttcc tgctctttgt tgaccagcgg gaaatacttg
     8641 tcagacagca gctcacgacc gcaaatcacc accagatcgt catcgtcctg gtagaccacg
     8701 tcgataagct cattgaccgc atccatcacc acggcgtcca ggttggcata ttcgccacct
     8761 ttcccgactt tcaccgcacc cggtatggtt tcaccgcccg tggtggtgct gcccatgacg
     8821 tgatccggtg catcctcacg gattttctgc agccagcctt tgttcacatc ctgcagcagc
     8881 gggttttcgc tacggttaga ggttttcgca cgcttcactc cgttaaagcc aatcatgatg
     8941 cggtccagtg cctgacgttt cacgatggcg ttacggatac gcacctggaa atcctgaaac
     9001 ttcgcccaca ggtccagctt cgcgtaggtc agcaccgtgt caaagttggt ctgttcgcat
     9061 ttgtattcca catcgaccat cagcgtcgga tcgacaggtt cacgctcttt cgcggtggtg
     9121 tcagtggttc cggcaatggt gctgccaaca cccagcccca gcagctgacc ggactgctca
     9181 gtcactggcg tgacgttaat cagcgtcagg aaagcggcgg actgctggat ctggtcttcc
     9241 agcgtctgct gcacagacgg ctctacagtg aacttgctgg acagttcttc aactgccaca
     9301 ccgttcagac gtgccagttg ctgcaggtaa gcgttaaaag caaagcgggt attcttcttc
     9361 atcaggtttt gtgctccatc agcaattggt cagagtgtca gcgggggcgt taccgcctgt
     9421 tgcacgctgg cggtagtcct ggcggctgtc ttcatgactc agcttattca ccagttcgtt
     9481 aaaggcggtt tgctgctcct gcagagcagt ctccagctca gacatgcgtt cttcctgctc
     9541 agacagggat ttttcggtgc gtgcgctcag gttctgctgc tcagtggcga ccagctccac
     9601 ggccttatgc acatcagaga accgggcgtc atcggactgc tcttttttgg taaacagcgc
     9661 cgtgacacgg gcaaacaggg acggcttgtc ctcctggatt tcttccagtt cgctcaccgt
     9721 ttcctctgca gcggtaaaaa gattggcggg attctgcttg cggtttgcca gcgggttata
     9781 ggctgcactg gcgctgaatg tcagcatttc agtgcccaga ctggcaggat catcagtggc
     9841 cgccaggccg actaggtagg ctttgcccgt atcagcaaac ttcgggctga cttccataga
     9901 ggtgaataat ttctggcctt ttttcaccag ctccaccagg gactccgttg gctcaacgtc
     9961 ggcatacagc gccatcttgc ccgccagcgg accttccgtg atttcttcag caaacagcgc
    10021 cgtcaccttg ccgtagcggt taaaggtgct gtccggcaga taagacttga tgtgctcaag
    10081 gttaatcagc gcggtataca ccgccgggtt gtagctggct gccatctgtt ccaaccattc
    10141 acgctggatt tcacgtccgt cggtagtggc accttccacc ccgatgcgaa aacgctttgc
    10201 tttcactgtc atgagccgtg ctccgttaga aaaaacttac tggagcctta tggttgcggt
    10261 gatgggggca gtgaaacaat gcgcggtatt tgtaccgaca accacacaaa ccgcaggcgg
    10321 ggaaagccgt cattcaaggc tgtaggtttg tgccatgaac accacactga cacccgcaga
    10381 tctcgatccc cgtcggcagg ccatgctgct gtactttcag ggataccgcg tagcccgcat
    10441 tgctgaaatg ctgggcgaga aagttgcaac cgttcacagc tggaaaaaac gcgacaagtg
    10501 gggtgactat gggccgctgg atcagatgca gctcaccacc gccgcactct actgccagct
    10561 cattatgaag gagcacaaag aagggaaaga tttcaaagaa attgacctgc tggcgcgcca
    10621 gtcggaacgc cacgcgcgca tcggcaagtt taacaatggc ggtaacgaag ccgacttaaa
    10681 ccctaacgtc gccaaccgca acaaaggccc gcgccgtcag ccggaaaaga atgttttcac
    10741 cgatgagcag attgagaagc tggaagaaat cttccattcc tccatgttca actaccagcg
    10801 ccactggtgg gaagccggaa aaaccaaccg catccgcaac ctgctgaagt cacgccagat
    10861 cggcgcgacc ttttactttg cccgtgaagc cctgattgac gcccttctga ctggacgtaa
    10921 ccagattttc ctttccgcca gtaaagcaca ggcccacgtc tttaaacagt acatcatcga
    10981 cttcgccaaa gaagtggagg tggagctgaa aggcgatccg atggtgcttc ccaacggggc
    11041 cacgctttac ttcctcggca ccaatgcccg cacagcccag agttaccacg gcaacctgta
    11101 tctggatgaa tatttctgga taccgaaatt ccaggagctg cgcaaagtgg cttccggtat
    11161 ggctattcac aaaaaatggc gacaaaccta tttttccacg ccatccagcc tgacacacag
    11221 tgcttatccg ttctggtccg gtgcgctgtt caaccgtggg cgcaacaaag ccgataaggt
    11281 ggacatcgac ctgtcccaca gcaatctggc ccccggcctg ctgtgcgcag acgggcaata
    11341 ccgccagata gtcaccgtgg aagatgcagt gcgcggcgga tgtaacctgt tcgacctcga
    11401 tcagttacgc atggagtaca gcccggacga ataccagaac ctgctgatgt gcgagtttgt
    11461 ggacgatctc gcgtctgtgt tcccactcag cgagctgcag gcgtgcatgg tggacagctg
    11521 ggaagtctgg accgactttc atgcactggc actgcgccca tttggctggc gcgaagtgtg
    11581 gatcggttat gacccggcga aaggtacgca gaacggcgac agtgccggat gcgtggtggt
    11641 ggcaccgcca gccgtgccgg gcggcaagtt ccgcattctt gagcgtcacc agtggcgcgg
    11701 gatggacttt cgcgcccagg ctgacgccat caaaaaactg accgaacagt acaacgtgac
    11761 ctatatcggt atcgactcaa ccggcgttgg tcacggggtt tatgagaacg tgaaagcgtt
    11821 ctttcctgcc gtccgggagt ttgtctacaa ccccaacgtt aaaaacgccc tggtactcaa
    11881 ggcctacgac attatcagcc accgccgtct ggagtttgac gccggacaca ccgacatagc
    11941 gcagtccttt atggcaatcc gtcgctccac caccgccagt ggcaaccgcc cgacctatga
    12001 agccagccgc agcgaagaag ccagccacgc cgatctggca tgggcaacga tgcacgcact
    12061 gtttaacgaa ccgctgcagg gcgaatccgc caataccagc aatattgtgg agattttttg
    12121 atgggaaaga gtaagaagaa ccgcgctgcg gcgacgaatc agatccagct taaaagtcaa
    12181 actacagccg aagcattcag cttcggcgat cccgttcctg ttctggaccg ccgagaatta
    12241 ctggactatg tggaatgcgt acagatggac cgttggtatg agccgcccgt cagctttgac
    12301 ggactggcgc gcaccttccg cgctgccgtg catcacagtt ccccgattgc agtaaagtgc
    12361 aacattctga ccagtaccta tatccctcac ccgctgctca gccagcaggc tttttcacgt
    12421 tttgtgcagg actacctggt atttggtaac gcctacctgg agaaacacac gaaccgcttc
    12481 ggtgaagtta tcgcccttga gcctgccctg gcaaaatata cccgacgcgg gttagaccta
    12541 gatacctact ggtttgtgca atacggtatg acaacccagc cgtatcagtt cacgaaaggc
    12601 agcatttttc atctgatgga accggatatt aatcaggaga tctacggcct gcccggctat
    12661 ctttctgcca tcccatccgc tttgctcaac gagtccgcca cgctgttccg ccgcaaatat
    12721 tacattaacg gtagtcatgc aggcttcatc atgtacatga ccgatgccgc gcagaaccag
    12781 gaggatgtga acaacctccg caatgcgatg aaaagcgcca aaggtcctgg caacttccgc
    12841 aatctgttta tgtactcgcc taacggcaaa aaagacgggc ttcagattat cccgttgtca
    12901 gaggtcgcgg cgaaggatga gtttctgaat atcaaaaatg tcagccgcga tgacatgatg
    12961 gctgcgcacc gcgtgccgcc gcaaatgatg gggattatgc ctaataatgt tggggggttt
    13021 ggggatgtgg aaaaggctag taaggtattc gtaagaaacg aattgatatc aatgcaaaaa
    13081 cggatcaagg agattaatgt ctggttaggt acagatatta tcgcatttac agaatataac
    13141 ttagatatgt aatcgtccca caatacaagg caaagagcat actctttgcc tgtaattaca
    13201 gttatacttt gcacctcttt gaataatctg caataatttc ctcaacacca tcaaaaataa
    13261 ctttaaactt ttcaaaacta attgttttac aatttgcctt tataaccttt gttgaaaaca
    13321 catgcttacc atattctgtt tttgtatccg tatcattaga tttattaaat gtctttccat
    13381 caagcaccgt tgatagtacc gtgctatcaa ataaatcctc catacaagac tctttccctc
    13441 caggaagcaa tggtgttaat aacaaataga gattgtcaaa tatgtgggta taccttgcct
    13501 tacgaatagt atcaacatca tttggacaat ttttacttta tcctttaagt gatttaataa
    13561 atctttaggg cctgagtcat tatcaagaag cataataaca gggtgctttg gtttaaattt
    13621 ctcgaacctc tttttttgtt cttggtatct taatataaat cttttgaagt ctgtagcacc
    13681 gccagaaatg tcaagaaaat agcttgtttt ttcagttgct ttaaaaaaat taacacgata
    13741 ctcattttta gagctatcat cagaagaatt aaacaataaa ggatatgaat tcgccaatga
    13801 cttcaaagca cactttaaat atacacggtc tgttttaccc tcagtgagta ttgttggcat
    13861 ttccattcca tgaaaatttt tatagtatag aaaataactg taggcttttt ctctcgaatt
    13921 caattttgca gtgtaattta atccatgatt tacactttgg tacttttcca actgtcttgc
    13981 attctttagt tttaaattat tatatttatc tattgagtca atgaatccaa aacggccttc
    14041 taatttagca catgtacctt tttcaatctt tcctgattca gtaacaataa caaaagaacc
    14101 atctttataa agagaatgag ccatggcacg cgttactttt gcatattctt tgccaacatt
    14161 cactttttta ttaacagtca acccagtaac atcttgtcgt gatgttttat aacaaaccct
    14221 tgtcttttta tcatt
//
LOCUS       DAZKIV010000121        14060 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZKIV010000121 DAZKIV010000000
VERSION     DAZKIV010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.121"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..710
                     /locus_tag="ACHOT6_003514"
     CDS             144..710
                     /locus_tag="ACHOT6_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948674.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            761..3220
                     /locus_tag="ACHOT6_003515"
     CDS             761..3220
                     /locus_tag="ACHOT6_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9948675.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGIL
                     MDYNLFASSYRPQDGSSSTNLNAYGTTGINAGAWRLRSDYQLNQTDSDDNHEQSGGIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDERMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDSVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDNQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3235..3966
                     /locus_tag="ACHOT6_003516"
     CDS             3235..3966
                     /locus_tag="ACHOT6_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFP9948676.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3963..5024
                     /locus_tag="ACHOT6_003517"
     CDS             3963..5024
                     /locus_tag="ACHOT6_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948677.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5176..6222
                     /locus_tag="ACHOT6_003518"
     CDS             5176..6222
                     /locus_tag="ACHOT6_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFP9948678.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAIAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6219..7010)
                     /gene="nei"
                     /locus_tag="ACHOT6_003519"
     CDS             complement(6219..7010)
                     /gene="nei"
                     /locus_tag="ACHOT6_003519"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFP9948679.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLTPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(7046..7780)
                     /gene="pxpA"
                     /locus_tag="ACHOT6_003520"
     CDS             complement(7046..7780)
                     /gene="pxpA"
                     /locus_tag="ACHOT6_003520"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFP9948680.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEQQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(7770..8702)
                     /gene="pxpC"
                     /locus_tag="ACHOT6_003521"
     CDS             complement(7770..8702)
                     /gene="pxpC"
                     /locus_tag="ACHOT6_003521"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFP9948681.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGSIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8696..9352)
                     /gene="pxpB"
                     /locus_tag="ACHOT6_003522"
     CDS             complement(8696..9352)
                     /gene="pxpB"
                     /locus_tag="ACHOT6_003522"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFP9948682.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9375..10118)
                     /gene="ybgI"
                     /locus_tag="ACHOT6_003523"
     CDS             complement(9375..10118)
                     /gene="ybgI"
                     /locus_tag="ACHOT6_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFP9948683.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVLRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10389..11870
                     /gene="dtpD"
                     /locus_tag="ACHOT6_003524"
     CDS             10389..11870
                     /gene="dtpD"
                     /locus_tag="ACHOT6_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFP9948684.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIVDQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11912..13330)
                     /gene="phrB"
                     /locus_tag="ACHOT6_003525"
     CDS             complement(11912..13330)
                     /gene="phrB"
                     /locus_tag="ACHOT6_003525"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFP9948685.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMISASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13327..13836)
                     /locus_tag="ACHOT6_003526"
     CDS             complement(13327..13836)
                     /locus_tag="ACHOT6_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFP9948686.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            13875..>14060
                     /locus_tag="ACHOT6_003527"
     CDS             13875..>14060
                     /locus_tag="ACHOT6_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948687.1"
                     /translation="MVNEFYGAEFLRGSILLVKILSTSSERELFYYFPSCIHFLYFLF
                     LENNSSQSLITSLLIVFQ"
BASE COUNT         3412 a         3746 c         3420 g         3482 t
ORIGIN      
        1 acatttaatt cataccatca attaattact tttacattta ataaaattaa taatattttt
       61 aagaaaaact aaaaacattc gacaatcaaa taagatattc ttgcaccgat atactttttt
      121 cttttctgtt aaaggactct gttatgttta aaggacaaaa aacattggcc gcactggccg
      181 tatctctgct gttcacagca cctgtttatg cggctgatga aggatccggt gaaattcact
      241 ttaaaggtga agttattgaa gcaccgtgtg aaatacatca ggatgatatt gataaagagg
      301 ttgaactcgg tcaggtgacc accagccaca ttaatcagtc acatcacagc gatgccgttg
      361 ctgtcgacct gcgcttagtc aactgtgatc tggaaaactc cagcaacggt tccggtggca
      421 agatttctaa agttgcagtc acctttgata gctcagcgaa aaccaccggc gcagatccga
      481 ttctcaacaa caccagcacg ggtgaagcca ccggcgtcgg cgtacgttta atgaataaag
      541 atcaaagcaa tatcgtttta ggcaccgcta ctccagatat cgacctggct ccgacctcca
      601 gcgaacaaac gctgaatttc tttgcctgga tggaacaaat tgatcaggct acacctgtaa
      661 cgccaggcgc agttacggcg aatgcaacct acgtgctgga ttataaataa gtattacttt
      721 ggcggcgcta tcgccgcctt tcataaaata tttatcaggt atggacaccg tgaatattta
      781 tcgactctct tttgtatcct gcctggtcgt agcgatgcct tgcgcattgg cggtcgaatt
      841 caaccttaat gttctcgata aatcgatgcg cgaccgcatt gatatttcat tattaaagga
      901 aaaaggagtc attgctcccg gtgagtattt tgttagcgtt gcggtaaata ataaccaaat
      961 cagtaacggg caaaagatta actggcacaa aaatgacgat aaaaccattc cgtgcatcaa
     1021 tgatttactg gtcgataaat ttggcttaaa acctgaagtc cgtcagtcgt taccattgat
     1081 aaatcagtgc gtcgatttta gctcccgacc tgaaatgctc ttcaatttcg atcaagccaa
     1141 tcagcaacta aatatcacca ttccgcaagc ctggctggcg tggcactcag aaaactggac
     1201 cccaccctcc acatggaaag aaggtgtcgc cggtatcctg atggattaca acttgtttgc
     1261 cagcagctac cgcccacagg acggcagcag cagcactaac ctgaacgcct acggtaccac
     1321 cggaattaac gccggggcat ggcgcttacg tagtgattat cagttgaatc agactgatag
     1381 cgatgataac catgaacagt caggcggaat atcgcgcacc tatctttttc gtccattacc
     1441 gcaattaggc tctaaattaa ccctcggcga aacggatttt agttccaata ttttcgacgg
     1501 tttttcttat accggcgcgg cactggcaag tgacgagcga atgttgccat gggaactacg
     1561 cggctacgcc ccacaaatta gcggtattgc acagaccaat gccacggtga cgatcagtca
     1621 atcaggccgc gtcatttacc agaaaaaagt cccaccaggc ccatttatca ttgacgacct
     1681 taatcagtct gttcagggca cactggatgt caaagtgacg gaagaagatg gtcgggtgaa
     1741 caatttccag gtttcggcag catcgacgcc cttcctgact cgtcagggac aggttcgcta
     1801 taaactggcc gcgggtcagc cacggccctc catgtcacat caaactgaaa atgaaacctt
     1861 ttttagcaat gaagtttcct gggggatgct gtcaaacacc tcgctgtacg gcggcctgct
     1921 gctttctggt gatgactacc attctgccgc aatgggtatt gggcaaaata tgctgtggct
     1981 tggtgcgctg tcgtttgatg tcacgtgggc cagtagccat tttgatactc agcaggacga
     2041 gcggggctta agctaccgtt ttaattacag caaacaagtg gatgccacta acagcacgat
     2101 ttccctcgcc gcttatcgtt tctccgatcg tcattttcac agctacgcca actatctgga
     2161 tcacaaatac aacgacagcg atgcgcagga cgaaaaacag acgatcagct tatctgtggg
     2221 ccaaccgatt accccactaa acctcaatct ttacgccaac ctgctacatc aaacctggtg
     2281 gaatgcagac gcctccacga ccgccaacat cacggcaggt tttaatgttg atattggtga
     2341 ctggagagat atctctattt cgacgtcatt caatacaacc cattacgaag ataaagatcg
     2401 cgacaaccag atttacctgt cgatttcgct ccccttcggt aacggtggtc gggttggcta
     2461 tgacatgcaa aacagtagcc acagcaccac acaccgcatg tcgtggaacg atacgctgga
     2521 tgaacgtaat agctggggca tgtctgccgg actgcaatcc gaccgtccgg acaatggagc
     2581 ccaggtgagc ggtaactatc agcatctgag ttcagcgggt gagtgggata tttctggtac
     2641 ctatgccgcc aatgattaca gttccgtcag cagcagctgg agcggttctt tcaccgcaac
     2701 ccaatatggt gcagcgtttc atcgccgcag ctccaccaat gaaccacgcc tgatggtcag
     2761 caccgatggc gtggcagata ttccggttca gggcaatctc gactacacca accattttgg
     2821 cattgcggtg gtgccgttga tttccagtta ccagccttcc accgtggcgg tgaacatgaa
     2881 tgacttaccc gacagcgtaa cagttgcaga aaacgttatc aaagaaacgt ggattgaagg
     2941 cgcgataggt tacaaatcac tggcttcccg ttccggtaaa gacgttaacg tcatcattcg
     3001 caacgccagc ggtcagtttc ctcccctcgg agcggatatc cgccaggatg acagcggcat
     3061 tagcgtgggg atggttggcg aggaaggaca tgcctggtta agcggagtcg ctgaaaatca
     3121 aaagtttacc gtggtctggg gtgataacca gcattgctcg ctccatcttc ctgaacatat
     3181 ggaagacacc gcaaatcgcc tgattttacc ttgtcattaa ttaaggaaat gaaaatgaca
     3241 tttatgaaag gactacccct tttgttgttg gttgccagtc tgtgcagcca cgctgcacta
     3301 caacccgatc gcactcgtat tgtgtttaac gctaatgata aagctaccag tctgcgggtg
     3361 gataatcgta gcgataaact cccctacctc gcttattcct ggctcgaaaa tgaaaaaggg
     3421 gagaaaagcg atgatcttct ggtcgcccta ccgccgatac aacgtctgga accgaaagca
     3481 acaacgcagg tacggatagt aaagcaagcc tcgaccacaa aattaccggg cgatcgcgaa
     3541 acgctatttt tctacaatat gcgtgaaatt cctcccgccc cggaaaaaaa cagcgaccat
     3601 gccgttctgc aagtcgccat tcagagtcgt atcaaggtgt tctggcgacc ggctgcatta
     3661 cgcaagaaag caggagaaaa ggtcgaactg cagttacagg tcagccagca gggcaaccaa
     3721 ctgacactga aaaatcctac cgcgtattat ctgaccattg cctacctggg acgaaatgaa
     3781 aaaggcgttc tccccggctt caaaaccgtg atggttgcgc cttttagtac ggtcaacaca
     3841 aacaccggaa attatagcgg cagccagttt tatctcggtt atatggatga ttacggcgcg
     3901 ttacgcatga cgacgctcaa ctgcagcgga caatgccatt tacaagccgt ggaggcgaag
     3961 aaatgagtgc tggcaaggga gtgttgctcg tcatctgtct gttatttctg ccgttgaagt
     4021 ctgcgcttgc cctgaactgc tactttggtt catccggtgg ctcggtagaa aaatcagaag
     4081 cgattcaacc ttttgctgta ccaggcaatg ccaaactcgg cgataagatt tgggaatctg
     4141 acgatattaa aattcccgtc tattgtgaca acaataccaa cggcaatttt gaaagtgagc
     4201 atgtttatgc ctgggtaaac ccctacccgg gggtgcagga tcgctattat caactcggcg
     4261 taacttacaa tggcgttgat tatgacgcca accaaggaaa aagccgcatc gacactaacc
     4321 agtgtattga cagtaagaac attgacattt atacccctga gcagattatc gcgatgggat
     4381 ggcagaacaa aatttgctcg ggtgatcccg ctaatattca catgtcgcgt acctttctcg
     4441 ctcgcatgcg gttatacgtc aaaatacgag aaatgccgcc gcatgattat caaagtacgc
     4501 ttagcgacta tatcgtcgtg caatttgacg gtgccggtag cgttaacgaa gaccctactg
     4561 cccaaaacct gaaatatcat attactggtc tggaaaacat tcgcgtgctg gattgcagcg
     4621 tcaatttttc catttcaccg gaaacacagg tgattgattt tggtaaattt aatttgctgg
     4681 atattcgtcg ccacacaatg tcgaaaacat tcagtattaa gacgacaaaa tcacaaaatg
     4741 accaatgcac cgacggattt aaagtcagtt cctcctttta taccgaagaa acgttggttg
     4801 aagaagataa agcattgttg attggtaatg gtctgaagct acgtttgctg gatgaaaacg
     4861 cctcgcctta taccttcaat aaatatgctg aatacgccga tttcaccagc gacatgttgg
     4921 tctacgaaaa aacctataca gctgaacttt cgtccattgc aggtaccccc atcgaggctg
     4981 ggcccttcga tacagtggtg ctttttaaaa ttaactataa ctgatttgcc atgatgccct
     5041 ttgcagccgg aaagggcatc tctttcagaa aattgctgat tttccccttt tccttgattg
     5101 ctgttatttt ttcctggccc ggctttcttt atgctgtatc gatacaccaa agagaaaata
     5161 atgagggagc gaaggatgcc agttttgcag tggggaatgt tatgtgtgct atcacttctc
     5221 ctttctattg gtttcctcgc ggtacatctc ccggcggcgc tattacttgg gccgatgatc
     5281 gccgggatca tcttcagtat gcgcggaatt actctgcaac ttccccgctc cgcttttctc
     5341 gccgcgcagg ccattcttgg ctgcatgatt gcgcaaaacc tcactggttc gattctcacc
     5401 acgctggccg ccaactggcc gatcgtgctt gctattttac tggtgacgct gctttccagc
     5461 gccatcgtgg gttggttatt ggtgcgctat agctcactgc ctggaaatac cggtgcctgg
     5521 ggttcttctc caggcggcgc agcggcaatg gtcgctatgg cgcaagatta cggcgcagat
     5581 attcgcctgg tggcgtttat gcaatatctg cgagtgctgt tcgtcgcggg ggccgctgtt
     5641 ctggtcaccc gcatgatgct gggtgataac gctgaagcgg ttaaccagca aatcgtctgg
     5701 ttcccgccag tgagcattaa tctcctgctt acgattttgc tcgcagtcgt tgccggtacg
     5761 gcgggatgca tgctgcgtct tccttccggc acgatgctca tcccgatgct ggcgggcgca
     5821 gtgctccagt ctggtcagct catcaccatc gaactaccgg aatggctgct ggcgatagcg
     5881 tatatggcaa ttggctggcg gattggtctt ggtttcgata aacaaatctt actgcgggca
     5941 ctacgcccac taccgcaaat cctgctgtcg atttttgctc tgctggctat ttgtgcaggt
     6001 atggcgtggg gactgacccg gtttatgcat attgatttta tgactgccta cctcgccacc
     6061 agtcccggcg ggcttgatac cgtggcggtc atcgccgcag ggagcaatgc cgatatggcg
     6121 cttattatgg cgatgcaaac cctgcgcctg ttcagtattt tgctgacggg gccagccatt
     6181 gcgcggttta tttcaaccta tgcgccgaag cggtcggcct agtgctggca gcctggacac
     6241 cagtaaaacg ggcgcgagga caacgtggtt ttctcaatga tgccgccaca gcgttcgcac
     6301 agttcgccat cacgatgaaa aaccttaaag cgaaacagcg ccccatgatg cttattctca
     6361 tccacctgcc cccgcgtagc gtaagaaaac cgaggaatct ccagtaacgc gtgtgcgagt
     6421 gcatccagtt gcgccgcatt gagatctttc gctttatgat ttccagtcaa cccaacctgc
     6481 cagaggatct ccacccgcaa ataattgcca agcccagcca gaaacgcctg atcgagcagt
     6541 aatcctgcaa actgacggtt acgaaagcgc ggtgacaata accgttcctt taccacctcc
     6601 ggcgtcagat tcggatccag cacatcggga ccaacgcgtt gtaaaaacgg atgcgtggtc
     6661 agttgttccg gggtcaacat ctcaatatcc gaggcgctat aaagcagaat ggttttgtca
     6721 gccgtttgca gttttacccg caatactcgc gtggtctgcg gctcttcgcc ggtatcaacc
     6781 acgcgccaga cgccgtaaag ctgattatgg ctgtagagcg ttaagtcgtt ggaaaaatga
     6841 gttaacaacg ccttaccacg cgtttccaca tgggtaacgt gttgaccgat aagttgtgat
     6901 tgataagttt ttaactgcgg gaaggcaaac cagacatcag ttagtggttt gcctttgatc
     6961 gccgcctcca ggttatccgc tgcacggcgg atctccgggc cttcaggcat ggtgatatcc
     7021 ttttttcatt ctttattaag cggggttatg ctgcgacaac aatccccttt ttggcaaatg
     7081 tagctcgtaa tcggcgggcg aacgccagag cgtgctcgcc gtcgccatgc aggcagaccg
     7141 tttgcgccgc gaccgttgcc cattcgccgg tgatactttt gactctgccg tgttgcacca
     7201 tctccagcgt ttgcgccagc gcctgttgtt cgttttcaat caacgcgcct ggctgacttc
     7261 gcggcaccag cgagccgtca gcctgataac cgcgatcggc aaacacttcc tcgcgcgttg
     7321 tcaggccata ttgcttgcct gcacgaatca gctcgcttcc tgccagccca acgagaatca
     7381 atgccggatc gcaagcgtat accgctctgg cgatggcgtc tgccagttgt gcttctttcg
     7441 ccgcctggtt gtacaacatg ccgtgcggtt tgacatgacg cattacgccg ccttgcgcac
     7501 gggcaatcgt tgccagcgcg ccaatttgat acagcgtctg ggcgtaaacg gtttctggcg
     7561 gcagctgcat ggcgctgcga ccaaaatttt ccctgtcggg aaaactcggg tgagcgccaa
     7621 tcgcgacacc attttttatt gcttcacgca cgcaagcctg catggtttgg gcatcgcctg
     7681 catgaaatcc acaagcaata ttggcagagg aaaccagcgt taatagctct gcgtcgctgg
     7741 cgcagccttc gcccagatcg gcgttcaggt caattttcat tgtgtagccg ccacgctaat
     7801 tgttcgaaat aacgttgttg atcttgccgc gctttcagtg cctcttccag tgaacactgg
     7861 acaaaatgaa tcggctgacc gagcggaatt tgcgccagat ggtacatatc agcctcaatg
     7921 atacaggcaa tacgcgggta accaccggtg gtctgtgcgt cgttcatcaa cacaataggc
     7981 tgcccgttat gcggcacctg caccacaccc ggtaacaaac cgtgagataa cagttcgcga
     8041 tcggtggtgc gttttaaaat ttgcccctgt aagcgatagc ccatgcggtt actttgcggg
     8101 ctaagctgcc agggcgaacg ccagaatgca tcctgcgagg cgcgatcgaa ctcatgatat
     8161 tccggccccg gcaaggcgcg aatgcggttg ccccacagca gctgtttaac gccctgcgct
     8221 tccataaaat cacgcttcga tttgccaatc gggagtcggt caccatcctt cagtaaacgg
     8281 ccttccagcc cgccaatccc cacgttgaga tcggtgctgc atgaccccat taccggcgga
     8341 acatcaatac tacccgcgac cgccagataa ctgcgcatgc cgtgctgcgg gcgtttaagc
     8401 gttaaacgct ggcctgcttt catcggcaag cgccagccgg tccagacggc gttatcatcc
     8461 agccgcgctt cgcaaccggc acccgtcaga gcaaaccacc catcagtttc gaactcaacc
     8521 gttaactgac cgagcgtgat ctccagcgcg ggggcattgg cgtcattacc caccagcagg
     8581 ttagcaatgc gtaacgcagg catatccagt gcgccgcagt ggctgatacc cgactggcga
     8641 aaaccgtgac gaccgccatc ctgcacagtg gtatacatgc ccgcacgaat aatcttcagc
     8701 atactccctc cttctgcggt acaaagcgca cgctgtctcc cggacgtaat aagatgggtt
     8761 cgtcacgcgc cggatcaaac aggctgagtg aggtatgacc aatcaactgc cagccacccg
     8821 gcgttgccag cggataaaca ccagtctgcg gcccgccgat cccgacagaa cctgccggaa
     8881 cgagcaagcg cggttcagcg cgccgtggcg tgtgtagttg ttccggcaaa ctcccgagat
     8941 acgggaagcc cggttgaaaa cctaaaaacc agaccacgta ttccacggag gagtgcaatt
     9001 caacaacctg tttttcgctc aacccgcaat gcgccgcgac caccgccaaa tccggtccgc
     9061 ctgcaccacc gtaaaccacc ggaatttcaa taaagcgaga ctcaggttcc agggcctcgc
     9121 tctcctccca ccagcgttgc aaacgctcta tggcatccag cgccagcgac tcaggattac
     9181 gcaaaatcac cgtgatattg ttcatgccgg gaatggcttc aaccacattc ggcatatcca
     9241 ccagacgctg cgccagtcgc cagatccgtt tctggctagc cagcgtcacc ggcggttcca
     9301 gttccagcac taccgccgtt tcacctatca gataacaacg cgctcgttgc actttcgatc
     9361 cctctgatta ttcgttatgc aggattagga atatcaataa aggtcacatc aagatcggta
     9421 ttttcattca gccactcgct caatgcgcga ataccaccac gttcagtggc atggtgacct
     9481 gcagcataaa aatgcaatcc ctgctcgcgg gctgaatgaa tggtctgttc agaaacttcg
     9541 ccagtaataa aagcatccac gccaaaacgc gcggcgctat cgataaaact ttgcccgccg
     9601 cccgtgcacc aggcgacgcg caggaccacc tcaggtccgg tatcgccaca ccataacggc
     9661 ttacgtccca gacgcgcttc aatccaggag gctagttcca gtcccggcac aggcatggtc
     9721 agctctcccc acggcaccaa cggctcaatt tcgcccatga ccgtgatccc cagtaacgcc
     9781 gccagctgtg cgttattgcc cagctcagga tgtgcgtcaa gcggcaaatg ccagccatac
     9841 aggttgatat cattcgccag caacgttttt aaacggttac gcttcattcc gcgaatgacc
     9901 ggagactctc ctttccagaa gtagccatga tgcacaatga ccgcatccgc gcccagacgc
     9961 actgcctcat cgagcaaagc ctggctggcg gtgacaccgg taacaatttt ttgcaccgtc
    10021 tctttgcctt ccacctgcaa accattcggc gcgtaatcac taatcgccgc gctgttcagt
    10081 ttttcgttaa tcagttgttc cagttcggtg tttttcatct tcgcgccttc cgtattctga
    10141 tagctacacc gttaacatag cgttacctcg cctgttcgtc gatagccaat cttttgacat
    10201 attaagaaac aaacccgacg ccaaaagacg caaattgttt atatcatcac gcttcagcta
    10261 atgtatattt atgcaatcag acagcatatt gcataaataa tcagatctgt atcaaagttt
    10321 taactttaag gaaacacaag tacgccgcgc aactattacg tgacgttgcc gtcaattgtg
    10381 gaattattat gaataaacac gcatcacagc cgcgcgctat ttactatgtc gttgcgctgc
    10441 aaatctggga atattttagc ttttacggca tgcgtgccct gctgattctc tatctcacca
    10501 atcaactaaa atacaacgat actcacgcct acgagttatt tagcgcctac tgttcgctgg
    10561 tgtatgtcac gccaatcctc ggtggctttt tggcggataa agttctcggc aatcgcatgg
    10621 cggtgatgct gggggcgtta ttgatggcga tcggtcatgt ggtgttgggt gccagtgaga
    10681 tccatccgtc attcctctat ctgtccctgg cgataatcgt ttgtggctat ggtctgttta
    10741 aatccaatgt cagttgcctg ctcggcgagc tgtatgagcc aaccgatccg cgtcgtgatg
    10801 gcggtttctc gctgatgtat gcggcgggta acgtggggtc tattatcgca cctatcgcct
    10861 gtggttacgc ccaggaagag tacagctggg cgatgggctt tggcctggcg gcggttggca
    10921 tgatcgcggg tctggtcatt ttcttatgtg gcaatcgtca tttcactcat acccgcggcg
    10981 ttaacaaaaa agtactgcgt gcgacaaact ttctcctgcc gaactgggga tggctgctgg
    11041 ttctgctggt ggcaacgcct gcactgatta ccgtgctgtt ctggaaagag tggtcggtat
    11101 acgccttaat tgtcgcgact attattggcc tgggtgtact ggcaaaaatt tatcgcaaag
    11161 cagaaaacca gaaacagcgg aaggagctgg gactgattgt gacgctcacc ttcttcagta
    11221 tgttgttctg ggccttcgca caacagggcg gtagctcgat tagcctttat atcgaccgct
    11281 tcgttaaccg cgatatgttt ggttataccg ttccgaccgc gatgttccag tcgattaatg
    11341 ccttcgcagt tatgctgtgc ggtgtgttcc tggcgtgggt ggttaaagaa agcgtcgcag
    11401 gtaatcgtac cgtgcgcatc tggggaaaat ttgctcttgg ccttggcctg atgagcgccg
    11461 gattctgcat tctgacctta agcgcccgct ggtccgcaat gtatggtcac tcttctctgc
    11521 cactgatggt gttaggcctg gcggtgatgg gctttgcgga actgtttatc gacccggtag
    11581 ctatgtcgca gattacgcgc attgaaatcc ccggcgtgac cggcgtatta accggcattt
    11641 atatgctgct ttccggcgcg attgcgaact atctggcggg cgtgattgtc gatcagacat
    11701 cgcaggcttc gtttgatgct tccggggcga tcaactactc catcaatgca tatattgaag
    11761 tatttgatca aattacctgg ggcgcactgg cgtgtgtagg actggtactg atgatttggc
    11821 tgtatcaggc gctgaaattc agaaaccgcg cgctggcgct ggaatcttaa taggccagca
    11881 ccatacctga taaaacgctc gctgcgtttt atcaggctcc tttcctcgct tcttcgtacg
    11941 ctgccagcgt tcgcagcctt gcctctttgt gatcaactat cggttgcgga taatccagca
    12001 tcacacctgc tttctgcgcc cacttccacg gctcatgcac cgctttccct ggtacatcgc
    12061 gcagctccgg taaccatcgg cggataaact caccttcgcg atcaaatttc tcgccctggg
    12121 ttgtcgggtt gaaaatacga aaatacggcg ctgcatcagt acccgtagac gccgcccact
    12181 gccagccacc gttattggct gccaaatcac catcaatcag ctgcgacatg aaatatcgct
    12241 cgccttcgcg ccagtcgatc aacaaatctt tcaccagaaa actggctgaa atcatccgta
    12301 gccggttatg catccagcca gtgctgttaa gctgacgcat ggcggcatcg acaatcgggt
    12361 atcccgtttt gccttcctgc caggcctgta aatgtgcggg attgctctgc cactgcacac
    12421 gatccgtcca ggcaataaac ggacaatgtt tacacaacga ggggtaatac gtcatcagat
    12481 gacggtagaa ctcgcgccag atcagctcac taagccagac actaccggcc ccaccgtcca
    12541 gcgcctgcgg ctgttccgcc aacaagcgat gcaaacactg gcgaggcgat aacccgcccg
    12601 ttgccaggct ggcggacaaa cgactggtgc cttccactgc cggaaaatct cgttgttgct
    12661 catattctcc ggcaccgttc tggcaaaact ggcgtaattg cgcaatcgcc gctttttctt
    12721 ccaccgggaa atgcgcagta tcgaaagact gacgaggata attcagcgta atggatggcg
    12781 agggctctat cgatccgcta ctacgaactt ttggcgcagc gacgcactcc ggcatccctt
    12841 cccgcagccg tttcagccag gcattcttaa aaggcgtaaa gactttgtac atctcgtgat
    12901 taccggtcat caccgcgcca ggcggcagga tcacgctgtc atcaaatcct tcacacacca
    12961 cgttacgcag cgctctttca acttccacat cccgcgcccg ctcattcact tcatactgat
    13021 agttataaaa caggtgggta acgctgtttt ccgcgcacac ctgtttaact atttcgacac
    13081 tggcgacaaa gtcatccact tcacggaaca ataaaggaat acctttttcc gcaagcgcta
    13141 tttgtagccc attcagttga gcattgataa gttcagcctg acgcggcgac atgttatgcg
    13201 tcgcccactg gcgtggtgta gcgatataca acgccagcac gcgtgcagac gaattgcggc
    13261 aggcggcagc cagtgcgaga ttatcgtgca gacgtaaatc ctggcgaaac cagaccagat
    13321 gggtagtcat aaaactcctg aatggcgcaa gttaatcaca cgcggccaaa gttcgaaata
    13381 acgctgccct gaaagccagg cgtcaggata aagcgccata tagtgtttga tacgcatcag
    13441 cggcgcaaga agtggttgct cgccacggcg ataactgtcg ataagcgcag ccagctgctg
    13501 gcgttctgtg gaatcaatat gcgggcgaaa ataaccctga acgtgcatca aaacattggt
    13561 gtgatcgcgc gggttggcgg ggtgagaaag caaaacaact acgcgttggc ggtactggtt
    13621 gtaatagtca tccagatttt gccactggtg aatatcagcc acgaacgggc caagtttgcg
    13681 gtactccggc tgagaatgcg ccagaaagac gagtttatag cggctgtgat aatcgagaag
    13741 cgccccgcgc gttaagccat gttctttcag tcgatgtaac tcatgaaggg caaagatacg
    13801 gattagggtg ctgtcatcaa atcgttgtag attcattact gctcctgaac ggtataaagg
    13861 agtaagtgta gacgatggta aatgaatttt atggggcaga gtttttgcgg ggaagtattt
    13921 tgttggtaaa aatattatca acttcatctg agcgagaatt attctactat ttcccgagct
    13981 gcattcattt tttgtatttt ttatttttag aaaataacag ttctcaaagc ttaattacct
    14041 cactattaat tgtatttcaa
//
LOCUS       DAZKIV010000122        14041 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZKIV010000122 DAZKIV010000000
VERSION     DAZKIV010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.122"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..1373)
                     /gene="plaP"
                     /locus_tag="ACHOT6_003528"
     CDS             complement(15..1373)
                     /gene="plaP"
                     /locus_tag="ACHOT6_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFP9948688.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1363..1425)
                     /gene="yoeI"
                     /locus_tag="ACHOT6_003529"
     CDS             complement(1363..1425)
                     /gene="yoeI"
                     /locus_tag="ACHOT6_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFP9948689.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1640..2569)
                     /gene="yeeY"
                     /locus_tag="ACHOT6_003530"
     CDS             complement(1640..2569)
                     /gene="yeeY"
                     /locus_tag="ACHOT6_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9948690.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2615..3439)
                     /gene="yeeZ"
                     /locus_tag="ACHOT6_003531"
     CDS             complement(2615..3439)
                     /gene="yeeZ"
                     /locus_tag="ACHOT6_003531"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9948691.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3522..3776)
                     /gene="yoeB"
                     /locus_tag="ACHOT6_003532"
     CDS             complement(3522..3776)
                     /gene="yoeB"
                     /locus_tag="ACHOT6_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFP9948692.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3773..4024)
                     /gene="yefM"
                     /locus_tag="ACHOT6_003533"
     CDS             complement(3773..4024)
                     /gene="yefM"
                     /locus_tag="ACHOT6_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFP9948693.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4308..4358
                     /gene="hisL"
                     /locus_tag="ACHOT6_003534"
     CDS             4308..4358
                     /gene="hisL"
                     /locus_tag="ACHOT6_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFP9948694.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    4334..4457
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4504..5403
                     /gene="hisG"
                     /locus_tag="ACHOT6_003535"
     CDS             4504..5403
                     /gene="hisG"
                     /locus_tag="ACHOT6_003535"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFP9948695.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5409..6713
                     /gene="hisD"
                     /locus_tag="ACHOT6_003536"
     CDS             5409..6713
                     /gene="hisD"
                     /locus_tag="ACHOT6_003536"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFP9948696.1"
                     /translation="MSFNTIIDWSSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDSITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6710..7780
                     /gene="hisC"
                     /locus_tag="ACHOT6_003537"
     CDS             6710..7780
                     /gene="hisC"
                     /locus_tag="ACHOT6_003537"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFP9948697.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAILVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7780..8847
                     /gene="hisB"
                     /locus_tag="ACHOT6_003538"
     CDS             7780..8847
                     /gene="hisB"
                     /locus_tag="ACHOT6_003538"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFP9948698.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8847..9437
                     /gene="hisH"
                     /locus_tag="ACHOT6_003539"
     CDS             8847..9437
                     /gene="hisH"
                     /locus_tag="ACHOT6_003539"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFP9948699.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIDDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9437..10174
                     /gene="hisA"
                     /locus_tag="ACHOT6_003540"
     CDS             9437..10174
                     /gene="hisA"
                     /locus_tag="ACHOT6_003540"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFP9948700.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLSRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEDDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10156..10932
                     /gene="hisF"
                     /locus_tag="ACHOT6_003541"
     CDS             10156..10932
                     /gene="hisF"
                     /locus_tag="ACHOT6_003541"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFP9948701.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10926..11537
                     /gene="hisIE"
                     /locus_tag="ACHOT6_003542"
     CDS             10926..11537
                     /gene="hisIE"
                     /locus_tag="ACHOT6_003542"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFP9948702.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11635..12612)
                     /gene="wzzB"
                     /locus_tag="ACHOT6_003543"
     CDS             complement(11635..12612)
                     /gene="wzzB"
                     /locus_tag="ACHOT6_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFP9948703.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGPAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSDALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12759..13925)
                     /gene="ugd"
                     /locus_tag="ACHOT6_003544"
     CDS             complement(12759..13925)
                     /gene="ugd"
                     /locus_tag="ACHOT6_003544"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFP9948704.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKKQSP
                     IVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMVIKSTIPVGFTKSIKERLGIDNLIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAECFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERELATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
BASE COUNT         3508 a         3638 c         3578 g         3317 t
ORIGIN      
        1 attacaaaag atgattacgc tacgtcttcg tactgcggaa ccgggttacg gaaactcttg
       61 gtgacgcaag ccaggtaaat cagaccgata gccgcccaga tcagacccag aaccattgag
      121 ctttcttcca ggttaaccca cagcgcacca acggtcagcg caccacacat cggcaggaac
      181 agatactgga agtgatcttt cagcgtctta ttacgcttct cacggatcca gaactgcgag
      241 atgaccgaca ggttaacgaa ggtgaacgcc accaacgcac caaagttaat cagcgctgta
      301 gccattacca ggtcgaagtt gattgccagc agggcaatcg cgccaaccag gatgatgttc
      361 atcgccggag tacgccattt cgggtgtaca taaccgaaga agcttttcgg gaatacgccg
      421 tcacgaccca ttacgtacat cagacgcgct acgcctgcat gcgccgccat accggacgcc
      481 agtacggtaa tggtggagaa gatcagcgcg ccaacctgga acgctttacc cgcaacgtac
      541 agcatgattt caggctgtga cgcatccgga tctttaaagc gagagatatc cgggaagtac
      601 agctgcagga agtaagttgc aaagatgaag atcatgccgc caatcagcgc ggtcaggaaa
      661 atcgcacgcg ggatcacgcg ctctgcatct ttggtttctt ccgacaggtt gctgatgccg
      721 tcaaagccgg taaaggagaa gcacaggatt gtcgccccgg taatcatcgg gataacatgc
      781 gcatcgccag accagaacgg acgagtgctc gccagcgtac cagcgccttc accttcaaat
      841 acgccataaa caaccatgcc cagaatcacc gcgatcagca ctacctgcaa cacgacgatt
      901 acagtgttga agttcgctac ggatttcaga ctacgcaggt taaaggcggt cataaaggcc
      961 accagcgcca ccacaaacat ccacgatggg atggaaggca ctagagcttc aaaatagatt
     1021 ttcgccagca gaatgttgat catcggcgcg aacagatagt cgagcagaga agaccaaccc
     1081 accataaagc cgacagtcgg gctaatggat ttctgggcgt aagtgtatgc agagccagca
     1141 gaaggatagc ggcgaaccag cttcccgtag ctcagagccg taaacaggat cgcaatcaat
     1201 gcgaacgcat aggctgtcgg cacatgacca tccgtgaggc ctgaaacgat accaaaagta
     1261 tcaaacagcg tcatcggctg catataggca agacccatca ttacaaccgg aactaacgta
     1321 agcgttttac gcaattccac gcgagaggtg tttggagtaa cgttatgcga catggtcatt
     1381 ctcctttacg gtgataaccg tcgcgtaagc aaaaaattgc cccatttttt tggattcctc
     1441 agcgacaaca actgtcgatt tttagtaaat atctatccgg tacgaagccc ggcctcttgg
     1501 tatgaatgat tggtttgaag caaaaaataa ccgacgctga tgaaacgtcg gtttttagtc
     1561 attttttgac agcggcgcat tgtgcctaaa tgggggggaa atgacaagag aatgagaggc
     1621 ttgtcataat aatttttctt taaatggctg attttccgtc atcagatttt gcataaacac
     1681 cggcgatcgc actatttgct aaaatttcat cccgccacca gccgctggca agccctgcgg
     1741 tctgttcatt ccagccaatc cagacgggtt caaagagagt ttgggcgacc actttcttct
     1801 cgattaacgc gccactatcg agaaaccgtt gcgccagata acggggtaaa tagccgcagc
     1861 cgaggccgct aatttgcagc tccagcttgg ttttaaaatc gaaaacggta atggcttctt
     1921 gttcgtcgag aagttgtgag gccgtagaag cgcctgcctg cgcagtatcc cccaccacaa
     1981 tcgcccgata tcgcttaata atacgacggt ttaacggctc ttcttccaga gctaacggat
     2041 gatgaggcgc gaccgcaaag acttgttcca gatcgcccaa ccgtgaaaaa ccaaactcac
     2101 tggaagatgg tggctcatgc atcgcgccaa caataatatc cgctcgcccc tgagtcaggg
     2161 catcccagga accaccgagt acgccattga taaacttcag gcgcgtcacg ctatgatgtt
     2221 gataaaaagc ttcaatcagt ggcgcaagca gagaaaaagg aaacgtgtcg tccacgccaa
     2281 tcaccagctc gttttcccag ccttcatgca gtttgatcgc ctgcttttcc agttctcgca
     2341 cggtatgcag aacttcccgc cctttctcta ataacatttt tccggtacgg gtgaatttag
     2401 cgcggtgacc gctacgatca agcaattgaa tattaaggtc gctttccagc ttgtgaacgg
     2461 tgtaactcag agcggacggc gtcttataga gtttggccga cgccgccgca aaactgcctt
     2521 ctttttctaa ggcatcgagg atcatcaaca catccagcag tggtttcata cacgccccct
     2581 tgcagtgcat gcggcgtcct gctggcgtga tcgtttactc cagcggcatt accagcggat
     2641 cgggatactg gtattcaaat cccagttcat tacaaatccg actgccatca ataatcttac
     2701 ctttgccgct gtccagacta tttctgaact gcggaggctc cagccccagt aaacgggcca
     2761 tctgcggata gaaaacatta cgcgcagggt gagctggcgc acatatatta tagatgtgtc
     2821 cgcctttagg tgcctgtaac aacagagtga tagcgccaat aacatcttct aaatggacta
     2881 aattaacacc atgttcacca tcaggcgcgg tttttccggc aaagaagcgt ccgggatgac
     2941 gtcccggtcc gaccagcccc gcaagacgca gaatatcgac cgaagtaccg ggtaaattgt
     3001 gcagccagtc ttcgagttct tctaatactc gtccactgtt ggttaccgga ttacgcgggg
     3061 tagtttcttt caccgtgcct tgcgcgtcgc catagacaga tgttgagctg gtaaaaataa
     3121 ttcggggaat acgatgggcc agcgcgctat ccactaactc ttgtaccgct tgtaaataga
     3181 actcatcgcc ggagccgcta cgacgtgccg gaagcgtaat gaccagtgca tcggcatcca
     3241 tcagggcatc cagatcgtca gaatcgcaaa ctaactcagg ctccatgcga agcagatagc
     3301 tatcaatgcc actcattcgg gccgcttcga caccatcttg tgtggtttta ctcccggtga
     3361 cttgccagcc tcgcgctgaa agtgacatcg ccagcggcat gcctaaccac cctaacccga
     3421 caattgcgac ctttttcatc cgttatctcc tgatacacct tagatctata aggctacgct
     3481 agcgtatcaa aactgacaat tcattctatg aatgaatctg ttcaataatg ataacgacat
     3541 gctgcaatga gcagtgaatc gtcggtaacc gcgtatacca gacggtgctc ctctgtaatg
     3601 cgtcgggacc agaaacctga caaattatgt ttcaggggtt ctggcttccc cttaccttca
     3661 aatggcgttc tgcgggtatc tttgataagt tcattgatct ttttaacaat tcgcttatct
     3721 gtttcctgcc agtacagata atcgtcccat gattcctcag accagattag tttcactcaa
     3781 tgatgtcctt ttccgttcct ttgcctgatt tcaggctatc gattgagtcc atcaatctcc
     3841 gggcgttagc gggggaacgc agtagataag ccgtctcttc cagggagtta tattcttcga
     3901 gtgacatcag aacacaagcc tctccattct gacgagtaat aaggatcggg gcatgatctt
     3961 caacggcttt catcattgtt gccgacaaat tctgacgcgc ttcgctgtag ctaattgtac
     4021 gcatgtcaat ctcctctttt gtacagttca ttgtacaatg atgggcgtta attaactatt
     4081 tattaattag tttgtagatc aagatattgt cagtgagacg aaaaatccag gcttcgctat
     4141 tttgggtacc gtcagcttag aggacagtcc tcttagcgcc ctcctttccc cgctcattca
     4201 ttaaacaaat ccatcgccat aaaatatata aaaaagccct tgctttctaa cgtgaaagtg
     4261 gtttaggtta aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc
     4321 aatttaaaca ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg
     4381 aagacattca gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct
     4441 tccgggggct tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag
     4501 aaaatgacag acaacactcg tttacgcata gctatgcaga aatccggtcg tttaagtgat
     4561 gactcacgcg aattgctggc gcgctgtggt attaaaatca atcttcacac ccagcgcctg
     4621 atcgcgatgg cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc
     4681 ggtctggtaa tggacggcgt ggtagacctt gggattatcg gcgaaaacgt gctggaagaa
     4741 gagctgctta accgccgcgc ccagggtgaa gatccgcgct actttacctt gcgtcgtctg
     4801 gatttcggcg gctgccgcct ttcgctggca acgccggttg atgaagcctg ggacggcccg
     4861 ctctccttaa atggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc
     4921 gaccagaaag gcatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg
     4981 cgtgccggac ttgcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagct
     5041 aacggcctgc gcgaagtcga agttatctac cgctcgaaag cctgcctgat ccagcgcgat
     5101 ggcgaaatgg aagaatccaa acagcaactg atcgacaaac tgctgacccg tattcagggt
     5161 gtgatccagg cgcgcgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat
     5221 gaagtcatcg ccctgctgcc aggtgccgaa cgcccaacca ttctgccgct ggcgggtgac
     5281 caacaacgcg tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa
     5341 aaactgaaag cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa gatgatggag
     5401 tgatcgccat gagctttaac acaatcattg actggagtag ctgtactgcg gagcaacaac
     5461 gccagctgtt aatgcgcccg gcgatctccg cttctgaaag cattacccgc accgttaacg
     5521 atattctcga taacgtgaaa acgcgtggcg atgaagccct gcgtgaatac agcgcgaagt
     5581 ttgataaaac cacggttacc gcgctgaagg tgtctgctga ggaaatcgcc gccgccagcg
     5641 aacgcctgag cgacgagtta aaacaggcga tggcggtggc agtaaagaat attgaaacct
     5701 tccacactgc gcaaaaactg ccgccggtag atgtagaaac gcagccaggc gtacgttgcc
     5761 agcaggtcac gcgcccggtg gcttcagtgg gtttgtatat tcctggcggc tccgccccgc
     5821 tcttctcaac ggtattaatg ctggcaactc cggcgcgtat tgcaggttgc aataaagtgg
     5881 tgctgtgctc accgccgccg attgccgatg agatccttta cgcggcgcag ctgtgcggcg
     5941 tgcaggacgt gtttaacgtc ggcggcgcac aggccattgc cgcactggcg tttggtacgg
     6001 aatctgtgcc gaaagtggac aaaatcttcg ggccgggtaa cgcctttgtc accgaagcga
     6061 aacgtcaggt gagccagcgt ctggacggtg cggctatcga tatgcccgca ggcccgtcgg
     6121 aagtgttggt cattgccgac agcggcgcaa cgccggattt cgtggcttct gatttgcttt
     6181 ctcaggctga acacggcccg gactcacagg tgattttact gacgcctgac gctgatatgg
     6241 cgcatcaagt tgccgaagcc gtcgaacgcc aactggcaga actgccgcgt gccgaaaccg
     6301 cccgtcaggc acttagcgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag
     6361 agatctccaa ccagtacggc ccggagcacc tgatcattca gacccgcaac gcccgtgaac
     6421 tggtcgatag cattaccagt gccggttcgg tatttcttgg tgactggtca ccggaatcgg
     6481 caggtgatta cgcctccggc accaaccacg ttctaccgac ttacggttac accgccacct
     6541 gttccagcct cgggctggca gatttccaga agcgcatgac cgtgcaggaa ctgtcgaaag
     6601 aaggcttttc tgcgctggct tcgaccatag aaacactggc cgccgccgag cgcctgaccg
     6661 cccataaaaa tgccgttact ttgcgtgtta acgcccttaa ggagcaagca tgagcaccgt
     6721 gactattacc gatttagcgc gtgaaaacgt ccgcaacctg acgccgtatc agtcggcgcg
     6781 tcgtctgggc ggtaatggcg acgtctggct gaacgccaac gaatatccca cagccgtgga
     6841 gtttcagctt actcagcaaa cgctcaaccg ctacccggaa tgtcagccga aagcagtgat
     6901 tgaaaattac gcgcaatatg caggcgtaaa accggaacag gtgctggtca gccgtggcgc
     6961 ggacgaaggt attgaactac tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct
     7021 ctactgcccg ccaacgtacg gcatgtacag cgtcagcgct gaaaccattg gcgtcgagtg
     7081 ccgcacagtg ccgacgctgg aaaactggca actggactta cagggcattt ccgacaagct
     7141 ggacggcgta aaagtggtct atgtttgcag ccccaacaac ccaaccgggc aactgatcaa
     7201 tccgcaggat tttcgcaccc tgctggagtt aacgcgcggt aaggcgattt tggttgccga
     7261 tgaagcctat atcgagtttt gcccacaggc atcgctggct ggctggctgg cggaatatcc
     7321 gcacctggct attttacgca cactgtcgaa agcttttgct ctggcggggc ttcgttgcgg
     7381 gtttacgctg gcaaacgaag aagtcatcaa cctgctgatg aaagtgatcg ccccctaccc
     7441 gctctcgacg ccggttgccg acattgcggc ccaggcgtta agcccgcagg gaatcgtcgc
     7501 tatgcgcgaa cgagtgacgc aaattattgc agaacgcgaa tacctgattg ccgcactgaa
     7561 agagatcccc tgcgtagagc aggttttcga ctccgaaacc aactacattc tggcgcgctt
     7621 taaagcctcc agcgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca
     7681 gaataaacaa ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag
     7741 ccagcgcgtc attgacgcct tacgtgcgga gcaagtttga tgagtcagaa gtatcttttt
     7801 atcgatcgcg atggaaccct gattagcgaa ccgccgagtg attttcaggt ggaccgtttt
     7861 gacaaactcg cctttgaacc gggcgtgatc ccggagctgc taaagctgca aaaagcgggc
     7921 tacaagctgg tgatgatcac taatcaggat ggtcttggaa cacaaagttt cccgcaggcg
     7981 gatttcgatg gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtacagttt
     8041 gatgaagtgc tgatttgtcc gcacctgccc gccgatgagt gcgactgccg taagccgaaa
     8101 gtaaaactgg tggagcgtta tctggctgag caagcgatgg atcgcgccaa cagttatgtg
     8161 attggcgatc gcgcgaccga cattcaactg gcggaaaaca tgggcattaa tggtttacgc
     8221 tacgaccgcg aaaccctgaa ctggccgatg attggcgagc aactcactaa acgagaccgt
     8281 tacgcccatg tagtgcgcaa caccaaagag acgcaaattg acgtccaggt gtggctggat
     8341 cgtgaaggtg gcagcaagat taataccggc gttggcttct ttgatcacat gctggatcag
     8401 atcgccaccc acggcggttt ccgtatggaa atcaacgtca aaggcgacct ctatatcgac
     8461 gatcaccaca ccgtcgaaga taccggcctg gcgctggggg aagcgttaaa aattgccctc
     8521 ggcgataaac gcggtatttg ccgctttggt tttgtgctgc cgatggacga atgccttgcc
     8581 cgctgcgcgc tggatatctc tggtcgcccg cacctggaat ataaagctga gtttacctac
     8641 cagcgcgtgg gcgatctcag caccgagatg atcgagcact tcttccgttc actctcttac
     8701 accatgggcg tgacgctcca cctgaaaacc aaaggtaaaa acgatcatca ccgtgtagag
     8761 agtctgttca aagcctttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgacacc
     8821 ctgccctcgt cgaaaggagt gctgtaatga acgtggtgat ccttgatact ggctgcgcca
     8881 atctgaactc ggtgaagtct gccattgcgc gccacggtta tgaacccaaa gtcagccgtg
     8941 acccggacgt cgtgttgctg gccgataaac tatttttacc cggcgttggc accgcacaag
     9001 cagcgatgga tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac
     9061 cggtgctggg catctgctta gggatgcaac tgctggggag gcgcagcgaa gagagcaacg
     9121 gcgtcgactt gctgggcatc attgacgaag acgtgccgaa aatgaccgac tttggtctgc
     9181 cgctgccgca tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg
     9241 ggattgatga cggcgcgtac ttttactttg ttcacagtta cgcaatgccg gtcaatccgt
     9301 ggaccatcgc ccagtgtaac tacggcgaac cgttcaccgc ggcggtacaa aaagataact
     9361 tctacggcgt gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa
     9421 atttcctgga gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg
     9481 tctccatcag ggcgattacg gcaaacagcg cgattacggt aacgacccgc tgtcgcgatt
     9541 gcaggattac gccgcgcagg gtgccgaagt gttgcatctg gtggatctga ccggggcaaa
     9601 agatccggct aaacgtcaaa tcccgctgat taaaaccctg gtggcgggag ttaacgttcc
     9661 ggtgcaggtt ggcggcggcg tgcgtagcga agatgacgtg gcggcgttac tggaagcggg
     9721 cgttgcgcgt gtggtggtcg gttccaccgc ggtgaaatca ccagaaaggg tgaaaggctg
     9781 gtttgaacgt ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca
     9841 aggcaacaag caggtggcag tcagcggctg gcaagagaac tcgggcgttt cactggaaca
     9901 actggtggaa acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg atatctcgcg
     9961 cgacggcacg ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc
    10021 gcaggtggca tttcagtctt ccggcggtat tggcgacatt aacgatgttg ctgccctgcg
    10081 tggcactggc gtgcgcggcg taatagttgg tcgggcatta ctggaaggta aattcaccgt
    10141 gaaggaggcc atcgcatgct ggcaaaacgc ataatcccat gtctcgacgt tcgtgatggt
    10201 caggtggtga aaggcgtaca gtttcgcaac catgaaatca ttggcgacat cgtgccgctg
    10261 gcaaaacgct acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc
    10321 agcgatggcc gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt gatcgacatt
    10381 ccgttttgtg tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt
    10441 ggcgcggata aaatttccat aaactcgcct gcgctggcgg atccgacgtt aattactcgc
    10501 ctggcggatc gttttggcgt gcagtgtatt gtggtgggta ttgatacctg gtacgacgct
    10561 gaaaccggta aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact
    10621 cagtgggaaa cgctcgactg ggtacaggaa gtgcaaaaac gcggtgccgg agaaatcgtc
    10681 ctcaacatga tgaatcagga cggcgtgcgt aacggttacg acctcgaaca actgaaaaaa
    10741 gtacgtgaag tttgccacgt cccgctaatt gcctccggtg gcgcgggcac catggaacac
    10801 ttcctcgaag ccttccgtga tgccgacgtt gacggcgcgc tggcagcttc cgtattccac
    10861 aaacaaataa tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc
    10921 aggatatgtt aacagaacaa caacgtcgcg aactggactg ggaaaaaacc gacggactga
    10981 tgccggtgat tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc
    11041 cggaagcctt agacaaaacc atcgaaagcg gcaaagtcac cttcttctcg cgcactaaac
    11101 agcgactgtg gaccaaaggc gaaacgtcgg gcaatttcct caatgtggtg agtattgccc
    11161 cggactgcga caacgacacg ttactggtgc tggcaaatcc aatcgggcca acctgccaca
    11221 aaggcaccag cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg
    11281 aacaactgct cgccgaacgt aaatctgccg acccggaaac ctcctacacc gccaaactgt
    11341 atgccagcgg caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac
    11401 tggccgcgac tgtacatgac cgctttgagc tgaccaacga ggcgtctgat ttgatgtatc
    11461 acctgttggt attgttgcag gatcaggatc tggatttaac gacggtgatt gagaacctgc
    11521 gtaaacggca tcagtgagtt gcggggtaag cggatgcgat attgttgtcg catccggcaa
    11581 aaaaaaacgg gcaaggtgtc accaccctgc ccgttttctt taaagctgca aagattattt
    11641 cgcgttgtaa ttgcgtagag cattacgccc cagcacaatc cccgcgccaa ccatgccgcc
    11701 cagcagtacc gccagaatca aggtaattgc ctttttcggg ctatcgcgac gaataggtaa
    11761 cgtcggtttc atcacatagc ggtaagcatg gatatcaaga tcatcaacct ttaaactttc
    11821 gatatccagc aggttttgac gagtctgata gtagtttggc gagaaaacta gtgggcgggt
    11881 cgcttcatgc ttaatcatcg actccagcgc gtcacttccc agcaggaaca tcgtatcttg
    11941 cgtcacgtcc tgcgtctgtt gaatctgcgg ctttgtcacc tgcgcctgat tcgcatactg
    12001 caacgcttcc tgaatctgac ggatacgcag atctttctgc tcctgcgcaa ctacttcctg
    12061 agttttcaga gaatcctgca agtttttcat acgcagcgcg atgttgtctt tcaggtcttt
    12121 ttccagttct tcatttacct ggtcgtcaac ttgctggata tacttcgcca gctgtttctg
    12181 cgcaccttca ggtgtttcac caacataaga caccgctaaa ggtaatgact gatttttgac
    12241 ggtaggctca atggtgagct tctctgcttc ttcctggtta tccagcgttt ccgctaatgc
    12301 tgaaaatgca gtgctgtaac gtccgataag tgaagcctga atgtcggaga ctttcggtac
    12361 agccggaccg taaatcacat tcatcgcgtt ggtataacca gcgatttgcc ccacatcagg
    12421 ttgagtaacg atagctgtgg aagtccactt ttctttggca acggccagat accctatagc
    12481 cagggcaata gcaacaatga cagagatgat aattgtcatc ttgccgcgcc acaactgcac
    12541 cagtaaatca atcaaatcaa tctgttccgg gtcatggttt tgcccagaaa cattattatt
    12601 ttctactctc atcattaccc taacggacga aataatttta agtcgccata gtttagcgat
    12661 aatcctcgtg aatgctatag gataagtgat aaaaataatg aattgttccg taagtaacaa
    12721 aagataatca gggcgaaaat cgccctgatc acaggatgtt agtcgctgcc aaagagatct
    12781 cgggtataga ccttatctgc cacatcttta agctcttctg ccatacggtt ggaaataatg
    12841 acgtcggctt gttgtttgaa ggtggcgagt tctcgttcca ggcgagagtt gaagaatgag
    12901 tcttctttca tcactggctc gtatatgatc acttcaacgc ctttcgcctt aatacgtttc
    12961 ataatcccct gaatggaaga cgcgcggaag ttatctgacc cgctcttcat aatcagacga
    13021 taaataccca ccacttgcgg tttgcgtgac aaaatggcat cggcaataaa gtctttacgc
    13081 gtgcggtttg catcgacaat tgccgaaatc aggttattcg gcacagactg gtagttcgcc
    13141 agtaactgct tggtatcttt cggcagacaa taaccaccat agccaaacga cgggttgttg
    13201 taatggttgc caatacgcgg atcgagacaa acgccttcga taatctggcg agtattcagc
    13261 cctaaacttt ctgcatagct atccagttca ttaaagtatg ctacgcgcat cgccagatag
    13321 gtattagcaa acagtttaat cgcttctgct tcagtggagt cggtaaacag tgtagggata
    13381 ttttgcttaa tcgcgccttc ctgtaataac gccgcaaaac attctgcgcg ttctgaacgc
    13441 tcaccgatga caatacgtga cggatgaagg ttatcgtaaa gagctttacc ttcgcggagg
    13501 aattccggag agaaaataag gttatcgata cccagacgtt ctttaattga tttggtgaaa
    13561 cctaccggaa ttgtagattt aatcaccatg acagcattag gattaatttc tgtcacatcg
    13621 cgaatgaccg cttccacgct tgaggtatta aaataattcg ttttcggatc ataatcggta
    13681 ggtgtggcaa taataacgta atcggcattt ttatacgcgt catacttatc tgtcgtagcg
    13741 cggaaattga gatctttagt cgccagatac tcttcaatct ccttatcaac aatcggtgac
    13801 tgcttcttgt taagcatgtc cactttggcc tgaacgatat ccagtgcaac cacttcgtga
    13861 ttttgcgcaa tcagaatacc atttgaaaga ccaacataac ctgttcctga aattgttatt
    13921 ttcattagct ctgacttctt ccggttaaac atttagagtg gtcattaatc caccaccaca
    13981 acttcatcta ctgcgggatt tttaacgctg atgttgatga actgtcaagg cagcgatcct
    14041 g
//
LOCUS       DAZKIV010000123        13984 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZKIV010000123 DAZKIV010000000
VERSION     DAZKIV010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13984)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13984)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13984
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.123"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1226
                     /gene="yjcF"
                     /locus_tag="ACHOT6_003545"
                     /pseudo
     CDS             1..1226
                     /gene="yjcF"
                     /locus_tag="ACHOT6_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
     gene            complement(1380..3029)
                     /gene="yjcE"
                     /locus_tag="ACHOT6_003546"
     CDS             complement(1380..3029)
                     /gene="yjcE"
                     /locus_tag="ACHOT6_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFP9948705.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(3181..4530)
                     /gene="ghxP"
                     /locus_tag="ACHOT6_003547"
                     /gene_synonym="yjcD"
     CDS             complement(3181..4530)
                     /gene="ghxP"
                     /locus_tag="ACHOT6_003547"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFP9948706.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(5076..5540)
                     /gene="soxR"
                     /locus_tag="ACHOT6_003548"
     CDS             complement(5076..5540)
                     /gene="soxR"
                     /locus_tag="ACHOT6_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFP9948707.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5626..5949
                     /gene="soxS"
                     /locus_tag="ACHOT6_003549"
     CDS             5626..5949
                     /gene="soxS"
                     /locus_tag="ACHOT6_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFP9948708.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5952..7538)
                     /gene="pdeC"
                     /locus_tag="ACHOT6_003550"
     CDS             complement(5952..7538)
                     /gene="pdeC"
                     /locus_tag="ACHOT6_003550"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFP9948709.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWPGEQEQVMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7968..8249
                     /gene="yjcB"
                     /locus_tag="ACHOT6_003551"
     CDS             7968..8249
                     /gene="yjcB"
                     /locus_tag="ACHOT6_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFP9948710.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(8348..8884)
                     /gene="ssb1"
                     /locus_tag="ACHOT6_003552"
     CDS             complement(8348..8884)
                     /gene="ssb1"
                     /locus_tag="ACHOT6_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFP9948711.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            9139..11961
                     /gene="uvrA"
                     /locus_tag="ACHOT6_003553"
     CDS             9139..11961
                     /gene="uvrA"
                     /locus_tag="ACHOT6_003553"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFP9948712.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11996..12352)
                     /gene="yjbR"
                     /locus_tag="ACHOT6_003554"
     CDS             complement(11996..12352)
                     /gene="yjbR"
                     /locus_tag="ACHOT6_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFP9948713.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(12356..12772)
                     /gene="yjbQ"
                     /locus_tag="ACHOT6_003555"
     CDS             complement(12356..12772)
                     /gene="yjbQ"
                     /locus_tag="ACHOT6_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFP9948714.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12883..13596)
                     /gene="aphA"
                     /locus_tag="ACHOT6_003556"
     CDS             complement(12883..13596)
                     /gene="aphA"
                     /locus_tag="ACHOT6_003556"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFP9948715.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3729 a         3568 c         3339 g         3348 t
ORIGIN      
        1 caagttcatc acatacaaag aaaacaaaat catataatta cagcaaacat cataaaaaca
       61 cgttaattga caataaagcc ctctctcttt tcaagatgga tgatcatgaa aaagtgatag
      121 gtttgattca gaaaatgaaa agaatttatg atagtttacc atcaggaaaa atcacgaagg
      181 aaacggacag gaaaatacat aaacatttta tagatatagc tttatatgca aataataaat
      241 gtgacgatag aattacgaga agagtttacc ttagtaaaga aaaggaagta tccattaagg
      301 tggtatattt tataaataat gtcgccgtcc ataataatac tatcgaaatt ccacagacag
      361 taaatggtgg ttacgatttt tcacacctta gcctgaaagg catcgtgatt aaagatgaag
      421 atttatccaa ttcgaatttt gcaggttgct gactacaaaa cgctattttc caggactgta
      481 atatgtataa aacgaatttt tattacgcca taatggaaaa aatacttttt gataattgta
      541 ttctagatga ctcaaatttc gctcagataa aaatggccga cggaactcta aatgcatgct
      601 ccgctatgca tgttcaattc tacaatgcag caatgaatag agccaatatt aaaaacacct
      661 tccttgacta ttcaaatttt tatatggcgt acatggctga gataaatctt tataaagtaa
      721 tagcgccata tgttaattta tttaaagccg accttagttt ctctaaactc gatttaatta
      781 actttgaaca tgctgatctg tctcgcgtca atctgaacaa agcaatcctc cagaatataa
      841 acttaattga tagcaaactc ttttgtacgt ggctgacaaa tacattcctc gaaatggtta
      901 tatgtaccgg ctctaatatg gctaatgtta attttaataa tgccaattta agcaactgcc
      961 atttcaactg ttctatttta acaaaagcct gtatgtttaa tacccgtctc tatcgggtta
     1021 attttgatga ggctagcgtc cagggaatgg gcatttccat tctccgtggg gaggaaaata
     1081 tccccattga tagtgatacc ctggtaacac tacagaaatt ctttgaagaa gattgtacct
     1141 ctcatactgg catgtcacaa actgaggata atattaatgc agtcgctatg aagattactg
     1201 cagatattat gcaacacgca gattgaggtt aagtcgaaga acagccaatg aattgacttt
     1261 acaagagaga taacttattt gttttagaat catgaatgtt agcccgagct aacctgttaa
     1321 aggtgcaaaa gattgcacct ttttcagatt acctcgggcc tggttacgtc gctttcggct
     1381 tactgatttt cctcaattag caacgcttca agcaaatcga gatcgtgcag taatttttgc
     1441 agcgtttcgt tgctgatctc ccgcgtggcg cgcaggtggt acagttcagc acgttcagaa
     1501 cgcaatgccg ccagacggaa gcgacgctca aggttctctt cctgcacgga actttcaacg
     1561 tcgttacgtc catcggcgcg acgacgcagg ttaccaatga cgcgagaact gacctccgta
     1621 agcagctggt tatcgatatt ctcttcggta tccgccgcca gacgctcctc cattttctgg
     1681 atcgccacaa tcgccacttc tgccgttgcc gctcgcgcaa tacgttcttc tttcaattgt
     1741 tgcgaatggt ccgcgacttc aatgtgttgt agcagaatag gcaacatcac cacgccgaca
     1801 aacagcgaga agagaatgac gccagccgcc aggaacacca gctcatagcg cgccgggaag
     1861 acgttaccat ccggcaagag cagcgggata gagagcacac cggccagagt gatcgcccca
     1921 cgcaccccgg cgaaagacgc gatcaggatt tctcgtgtgg tccacgaacc aaactccatc
     1981 ggcttcttct tcaggaagcg gttgctgaac tttttcatcg tccacagcca gccgaaacgg
     2041 accagcatca gcgccgcata tatcagaata atatcggtaa acagcatcca gatttcgacg
     2101 ttagggtcga tttctgccgc catcagcgac gtctccagaa tacccggcag ctgcagacct
     2161 aacagcagga acaccatgcc gttaaagaca aattccagca tcgcccaggt gctgtttgcg
     2221 cgcaggcgca ttgccagcgg cgcacggcgc atcacaccgg agcgggtgat ggtcatcccg
     2281 gcggcaaccg cagcgaggat gccggaaacg ccaatatgtt cggcaatcag ataagaagcg
     2341 aatggcagca agaacagcag aacgatctgc gtcgcgggtt catcaccgcc ccagcggctg
     2401 aggaatcgca gcgagcgacc atacagccag ctcaccacaa aaccggcaag aataccacca
     2461 atggctactt tcataaattc gaccgtcgca ccgccgacgg tgaagatcat cgtccccatc
     2521 gccactgcca cggcaaactt caacgacacc aggccggagg cgtcattcat caacgcttcg
     2581 ccctgcaaaa tgcccatgat ttttttcggg atgcgccctt cgccaacaat cccggagagc
     2641 gccacagcat cggtcggaga aagtaccgcc gccagcgcaa aggcggggat cagcggaatg
     2701 cccggcacca cccagtaaat aaggaagcca atgccgacca cggtgaccac caccagcgcc
     2761 agcgcgaggc cgaaaatctc tcgaccatgt tcgagaaatt cacgggtcgg cgttttccag
     2821 ccatcagcga acagcaacgg cgggataaac aagactaaaa agagttcagg atcaaactcc
     2881 acatgcaaac caaacgtcgg ccacgccagt agcgcaccga tggcgatttg cataagcgga
     2941 agcgggatct gaaagggcat gacacgagtg accaccccgg acagcgagac cacgagggtc
     3001 attatcagta tggtgaagaa gatttccatg cgttccctgt ttgcgatgtt tttttatgat
     3061 ctgagaaagg cgttatgcct gaattctcat tttttcagaa tatcgcaaaa gctcacacaa
     3121 tgtgcggaga aaataaaaac gggcagccat tgccacccgt tttcagaatc cgatgaaaga
     3181 ttagatagcc cagccacccg catagaaggt caccagcgcc acggcgataa cgaccgtacc
     3241 gatgttcaac ttgcgccatt cgccggaaac cagacgacca atcaccagag tcgcgaagcc
     3301 tatcatgatg cctgttacga tgttacaggt cagcacgatg aataccgccg taaccagacc
     3361 cgccatcgca tcaacaaaat cagcaaagtc gattttcgcc acgttgctca gcatcagcag
     3421 gccaacgtac atcagcgccg gagccgttgc ataccccgga acgaggtaag agagcggaga
     3481 gaggaacaga atcagcagga acagcacgcc aacggtgata gccgtcaaac cggttttacc
     3541 gcccgccgcc gtacccgccg cagactcgat gtataccgct gccggagcag cacccaccag
     3601 gccagagaaa acgctgctca tggagtcagt ggtcagtgct ttcccaccgt cgatgatctg
     3661 cccatcttta tccagcaggt tcgcctggcc ggcgacggca cggatagttc cggtggcatc
     3721 aaatactgcc gtcatcacca gcgccagaac gcttggcagg actacaggat tcagcgcgcc
     3781 cataatgtcc aggctgccaa tcagtgaatt gccgttttca tcgctcaatg aaggcatggc
     3841 gaaaacgccg gagaaatgga cgttaggatc gaagatcaaa ccgacaattg agataccgat
     3901 aatggtcagc agaatgccac cagggacttt cagtttttcc aggccgatga tcaccgccag
     3961 acctaccagt gacataatca ccgggaaggt cgcgaaatca cccagcgcaa cgggcagacc
     4021 atcaagcggg tttttaatca ccagaccgac accattagcg gcaatgagca gcaagaacag
     4081 accgataccg atccccgtgc cgtgcgccac accgtgcggc aagttgcgca aaatccagct
     4141 acggataccc gtggcagaaa ttaccgtaaa cagcacaccc atcaggaaca cggcacccag
     4201 cgcgacaggt acgctaatat gttgccccag caccaggctg aatgcggtaa atgccgtcag
     4261 ggagatggcg caaccaatcg ccaacggcag attagcccac agacccatca cgatagaacc
     4321 gagtccggca accagacagg ttgcaacgaa aactgccgca ggcgggaagc ctgctttacc
     4381 caacatacct ggaacgacga tgaccgagta gaccatcgcc agaaacgttg ttaacccggc
     4441 aaccacttcc tgacggacag tgcttccacg ttgtgaaatt ttaaaccagg cgtcgagtga
     4501 accgccggta cgcgctgatg gcgtagacat aagaaaacat cccctgagag tttaattttc
     4561 gtcaagatgc gtggcggaca atcccctgcc agcgaaacgt tatcttcctg ctccaggttt
     4621 gcgacaattt actgcgtcgc aaaaaagcaa acgtttagct ccgcgcatac aaaacgggtg
     4681 gtaaaaaaag caaacgatta tccggcgatc atagcggctt tttcaaccga agtttgctgc
     4741 ttttttctct tttattgtct tgacgatgaa aaaacaggca attgatgcgc aaacgttatc
     4801 gtcggtgcgc aagattgcag cgttatcgag ccgtataccg ttcaggattg cgataaaaaa
     4861 tcgtctgaga tgacaaaaat attgcggtta aaaagtatac ttttacactg gcgaagaaac
     4921 gtcggtgaca tacaattaaa gcatcaacac caaccggaac ctccaccacg tgctcgaatg
     4981 aggtgtgttg acgtcggggg aaaccctcct gtgtaccagc gggatagaga gaaagacaaa
     5041 gaccggaaaa caaactaaag cgcccttgtg gcgctttagt tttgttcatc ttccagcaag
     5101 cgtgcgccgg taccttcttc tcctaagcgg tcgcccgggt tacgcaacgg gcaatcactg
     5161 cgcgaaaggc agccacaacc aatacatccg tccagttcat cacgcagcgc cactaaggta
     5221 tgaatgcgcc gatccaactc ttctcgccat tgggacgaaa gctgtttcca ctctttcgca
     5281 cttaacgtat gcccttcggg caacacgcca aacgcttcac caatggtcgc cagcggaatg
     5341 ccaatacgct gagcaatttt gataattgca acatatcgca acacatcacg tttatatcgc
     5401 cgctgattgc cgctgttacg gatactggta atcaaccctt tactttcata gaaatgcagc
     5461 gccgataccg ccacaccgct gcgtttcgcc acttcgccgg gggttagcag cgctttaatg
     5521 cggggtaatt tcttttccat aaatcgcttt acctcaagtt aacttgagga attatactcc
     5581 ccaacagatg aattaacgaa ctgaacactg aaaagaggca gatttatgtc ccatcagaaa
     5641 attattcagg atcttatcgc atggattgac gagcatattg accagccgct taacattgat
     5701 gtagtcgcaa aaaaatcagg ctattcaaag tggtacttgc aacgaatgtt ccgcacggtg
     5761 acgcatcaga cgcttggcga ttacattcgc caacgtcgcc tgttactggc cgccgttgag
     5821 ttgcgcacca ccgagcgtcc gatttttgat atcgcaatgg acctgggtta tgtctcgcag
     5881 cagaccttct cccgcgtttt ccgtcggcag tttgatcgca ctcccagcga ttatcgccac
     5941 cgcctgtagt tttattgccc gcgcgttaac tcccgcgcgg gtaattgctc catccattgc
     6001 ataaacacct gcggcggcat cgccttcgca aaaaaccatc cctggcaata gcgcacgccg
     6061 cgtttgcgca gccagttaac ctgctcctca gtttcgacgc cttcagcgat cgtttttaac
     6121 cccaggctgt gcgccagctc gatgatgtgt tccgcaatca aatgactggt tttgtgggtg
     6181 gtcagcgttt caacaaacga tttgtcgatt ttcaaaatat cgacattcaa tgatttaagg
     6241 ttatgcaagt tagagtagcc aataccaaaa tcatcaattg ccacttcgta acctgcctgg
     6301 cggaaagcca gaataatcgg cgtcattttg tcaacatcaa gaaatgcatg ttcagtcact
     6361 tcaaatttaa tttgctgcgg acgcaccgcg tattgctctg ttttctgatt gattcgcgct
     6421 atcaaccgtg acgtatgaaa atcggaggcc gacaggttaa tagaaacata gcgatctgca
     6481 tgtgttgcca ggtaatcgcc cagatcgcgg aagacattat caataacata atcagttacc
     6541 tgttctatca tcccctcctt ttctgccagc ggaataaact ctgccggatt cattacttgc
     6601 tcctgctcac caggccaacg taacaacgct tcagcgccga tacatttttc tgttttgata
     6661 tcgattattg gctggtaata aagacaaagt tgatgttttt ctagagcgcg ttgcaatttg
     6721 cgtttgggag acaaatagtt ttgtcgaata cgtagccaga gtaatagcag aacaagactc
     6781 cccaaaatac cggcgggcaa cgcaaaaata agatgattat aaaaatgggt tataagacgt
     6841 tgatatgaag tcgcaacaat ggctgcaatt gggcgttttg tcgaataaac tgtcgcatat
     6901 aaatagccat ttctttgtac ggttaaatcc ttcaaatgaa tcagcggaga aaacgttgct
     6961 gccgaggcct ctttgcttaa cgagaaaaag gttttcgtca ccgtatcata cacaccccat
     7021 tgcaatgtcg gatcatcaga catcacttca ctccagaaga gagggttgat aaccgccaca
     7081 taatttcccc gctgcatata ggtcatttta tagccagaga aaaaaggcgt atcgcggtaa
     7141 taatagatag aaacgttagg ttcacgctta taatcggccg gtgcaatcgt atagccgttt
     7201 acaggcgcta tcagcgatga gcataaaaaa tggttatcac gggcatagat caattcatta
     7261 atataaagat agccacgaat aatattcaac attcgctttt gatgggctgg agtgcaaact
     7321 tgcccctgat agcgttcagc tgcatcgctc actaaatctg cctggcgaat gaccagctcg
     7381 gatttatcca gtgccagttg agcaaaggtt cggagctgat tattcacttc tgattttgcc
     7441 caaaggaagg caatccatag cgaaagaatg atgggaaaga gaactaaaaa gataatgccc
     7501 ggcaacgcca gtaattggtg tcgtgcacga tgactcattg ctatcaattt cctgcctgtt
     7561 tatacccggc cattgcaagg tgaaaaccct gggtaccgac gtctttagat aaatcttacg
     7621 gtataaatag cacttttgcc tgatttgaaa ttgatacctt tctaatcgtc acctggatga
     7681 aaataattca ttgatattca aaattaattt cctacaggaa tgcagaggcg gcgggaagat
     7741 atgcccttaa tgccactgaa aaaccacata atctgtacag caataaaagt cacggcctaa
     7801 tatggctaac aaacaatcaa ttgtgatata gttcacaaaa ttaatgaaac aaacagagtg
     7861 tttcattttt gtgttctgca agccccgcga tagccagagt gtaccacgcc tcccgtgaac
     7921 aacgccgcgc tgtccagggc atcggctctt ttcagaggaa ttgtttgatg gctaccctca
     7981 ccactggcgt ggttcttctt cgctggcaac ttcttagtgc cgtaatgatg tttctggcca
     8041 gcacactcaa catccgtttt cgtcggtctg attatgtcgg gcttgcggtg atcagcagcg
     8101 gtctgggcgt tgtttccgcc tgttggttcg caatggggtt gcttggcatc acaatggcgg
     8161 acatcaccgc tatctggcac aacatcgagt cggtgatgat agaagagatg aatcagacac
     8221 cgccacaatg gccaatgatt ttgacctgat acgtagaagc ctccataacg gaggcttctt
     8281 ttttatggct ggcagatgct ttaatcatcc accttaaaac aatataacct attgttttaa
     8341 tgacaaatca gaacggaatg tcatcatcaa agtccatcgg cggctcgtta gacggcgctg
     8401 ccggagcgga ctgctgcggg cgagactgcg cgccgccgct gaactgattg ccaccctgcg
     8461 gctgctgagg ctgaccccaa ccgccctgcg gctgaccacc accgatatta ccacctgccg
     8521 gagcgccacc accctgacga ccacccagca tctgcatggt gccgccaacg ttcaccacga
     8581 cttctgtggt gtagcgatcc tgaccggatt gatcggtcca tttacgggta cgcagctgac
     8641 cttcgatata aacctgagaa cctttacgca gatattcgct ggccacttct gccagtttgc
     8701 cgaacagcac aacgcggtgc cattcagtct gctctttcat ctcgccggtc gctttatcac
     8761 gccaggattc ggaagtagcc agcgtaatgt tggcaactgc gccaccattt ggcatgtagc
     8821 gtacttccgg gtcctgaccc agattaccaa cgagaataac cttgtttacg cctctgctgg
     8881 ccatgttcgt gtctcctgaa aaaaaatcgt tctgaataag tgtaaacgcg cgattgtacc
     8941 attaccaata gcgcttttac tatgttgtga cctcggttcc gggaaacaaa cctggccaga
     9001 cattgttaca caacactccg ggtattgcat tccaatactg tatattcatt caggtcaatt
     9061 tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat gccaccgggc aaaaaagcgt
     9121 ttaatccggg aaaggtgaat ggataagatc gaagttcggg gcgcccgcac ccataatctc
     9181 aaaaacatca acctcgttat cccccgcgac aagctcattg tcgtgaccgg gctttcgggt
     9241 tctggcaaat cctcgctcgc tttcgacacc ttatatgccg aagggcagcg ccgttacgtt
     9301 gaatcccttt ccgcctacgc gcggcagttt ctgtcactga tggaaaagcc ggacgtcgat
     9361 catattgagg ggctttctcc tgccatctca attgagcaga aatcgacgtc tcataacccg
     9421 cgttctacgg tggggacaat caccgaaatc cacgactatt tgcgtttgtt gttcgcccgc
     9481 gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg cggcgcaaac cgtcagccag
     9541 atggtggata acgtgctgtc gcagccggaa ggcaagcgtc tgatgctgct cgcgccaatc
     9601 attaaagagc gcaaaggcga acacaccaaa acgctggaga acctggcaag ccagggttac
     9661 atccgtgctc gtattgatgg cgaagtctgc gatctttccg atccgccgaa actggaactg
     9721 caaaagaaac acactattga agtggtggtt gatcgcttca aggtgcgtga cgatcttacc
     9781 caacgtcttg cggagtcgtt tgaaaccgcg ctggagcttt ccggtggtac agcggtagtg
     9841 gcggatatgg acgacccgaa agcggaagag ctgctgttct ccgccaactt cgcctgccca
     9901 atttgcggct acagtatgcg tgaactggag ccgcgactgt tttcgtttaa caacccggca
     9961 ggtgcctgcc cgacctgtga cggccttggc gtacagcaat atttcgatcc tgaccgcgtg
    10021 atccaaaacc ccgagctgtc actggctggc ggtgcgatcc gtggctggga tcgccgcaac
    10081 ttctattatt tccagatgct gaaatcgctg gcagatcact ataagttcga cgtcgaagcg
    10141 ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt tgtacggttc tggcaaagaa
    10201 aacattgaat tcaaatacat gaacgatcgt ggcgatacct ccatccgtcg tcatccgttc
    10261 gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga cagaatccag tgcggtacgt
    10321 gaagaattag ccaagtttat cagcaatcgc ccatgcgcca gctgcgaagg gacgcgtctg
    10381 cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc tgcctgctat ctccgacatg
    10441 agcatcggtc atgcgatgga attcttcaac aatctcaaac tagctggtca gcgggcgaag
    10501 attgcagaaa aaatccttaa agagatcggc gatcgtctga aattcctcgt taacgtcggc
    10561 ctgaattacc tgacactttc ccgctcggca gaaacacttt ccggcggtga agcccagcgt
    10621 atccgtctgg cgagccagat tggtgcgggc ctggttggcg ttatgtatgt actggacgag
    10681 ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt tgggtacgct tatccatctg
    10741 cgcgatctcg gtaataccgt gattgtggtg gagcatgacg aagacgcaat tcgcgccgct
    10801 gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg gcggtgaagt ggtcgcagaa
    10861 ggtccgctgg aagcgattat ggcagtgcct gaatcgttga ccgggcagta catgagcggt
    10921 aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga atccagaaaa agtgctgaag
    10981 ctgactggtg cacgcggcaa caacctgaag gacgtgacgc tcacgctgcc ggtcggtctg
    11041 tttacctgca tcacaggggt ttcaggttcc ggtaaatcga cgctgattaa cgacacactg
    11101 ttcccgattg cccaacgcca gttgaatggt gcgaccatcg ccgaaccggc accgtatcgc
    11161 gatattcagg ggctggagca tttcgataaa gtgatcgata tcgaccaaag cccaattggt
    11221 cgtactccac gttctaaccc ggcgacctat accggcgtgt ttacacctgt gcgcgaactg
    11281 tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc caggacgttt cagctttaac
    11341 gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg tgatcaaagt ggagatgcac
    11401 ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag gtaaacgcta taaccgtgaa
    11461 acgctggaaa ttaagtacaa aggcaaaacc atccacgaag tgctggatat gaccatcgaa
    11521 gaggcgcgtg agttctttga tgcggtgcca gctctggcgc gtaagctgca aacgttgatg
    11581 gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa ccacactttc tggtggtgaa
    11641 gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg gcaccgggca gacgctgtat
    11701 attctcgacg agccgaccac cggtctgcac ttcgccgata ttcagcaact cctcgacgtg
    11761 ctgcataaac tgcgcgatca gggcaatacc attgtggtga ttgagcacaa tctcgacgtg
    11821 atcaaaaccg ctgactggat tgtcgacctg ggaccagaag gcggcagtgg cggcggcgaa
    11881 atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg aagcttcgca tacggcacgc
    11941 ttcctcaagc cgatgctgta atcgttaagg ccgctttctg agcggccttt tcctttcaga
    12001 gttgtaccag caatttacgc ttttcttccg gtagtaaatt cactgcctgc tgataagacg
    12061 catccaccag ataatagatt tgcgaatccg gcagcgaacc gtcgagatac acggtgctcc
    12121 agtgcgcttt attcagatgg cggctaggac gcacatcgct gtgctgctga cgtagcagct
    12181 ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg attttctact tctttcacca
    12241 tcgcaaacag tacatcttcc actttgatct gcgtcgcttt ccagtcgtta tgcacgctct
    12301 gttctgcgcc tggttttgcc atgcaatatt gtagcaactc cgaaatggtc attttttact
    12361 ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg gatgcgatgt tcccccagcc
    12421 agatgccttg ccaggtgccg gtctgaatac gccctttatg taacggcaat acaagcgatg
    12481 ttcccagcat tgaggatttg atatgagaag gcatatcgtc tgctccctca tagtcatgct
    12541 cataatttcc gttgtcggga acggtgcgga ggaaaaaacg ctccatgtcg tggcgtacgg
    12601 tgggatcgca gttctcatta agtgtcagag aggcggaggt atgttgcagc aacagatgca
    12661 gtaagccgat gttaacgcgc ggcatatcag ccagctgatt cagaatttca tccgttacca
    12721 gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt ttgataccac atgtgctgct
    12781 ccttgataaa actctcttaa tcagtttgca gcaagacagc gaaaggataa aggtgtgatt
    12841 aaaaaaacag cattcgggag agtgtcacgc tctcccgctc tgtcagtatt ctgaattgac
    12901 gatcacctct tcaccaaacg cacccgcttg tggcaagggt ttgtaggtag agttggaggc
    12961 gcgcagaatg cggataccac gagcgccgac atcgcgtgca gcggtaatat cgttatcgga
    13021 atcgccataa aaaattcgga tatttttatc ctgcagccat tgcgattttg tattttgccc
    13081 tggtttatcg cccgcaaaga tcaccggatt catgttggtg acaggaatat gaaaattatc
    13141 cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta cgaccagtca caaagaagat
    13201 cgcgtcaccg cggcgtacat gcatatcaat cagctggcga gcgacctctt ttggaatgct
    13261 gaattcatcc cagccattgt tcattttttc ccagaacaca ggatttttca gataatcttc
    13321 gctttctggt gagaaggttt ttttgccgcg ccagaagccc gggctggaaa aaagtaccgt
    13381 gtcatcgata tcaaacccca ccgccattgg cggacgccct gcgaggctat tttcaatttg
    13441 tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc ctggcaacgt tagtcccagg
    13501 gttaagcggt gaaggagatg aggccagggc aacagcggaa ctgtttagag cgaacaataa
    13561 gcaaacggca ctgattgcct gtgtgatctt gcgcatattt ttccctaaat agtcagtatg
    13621 ttgaaacttt ttattgtgag atttgttgca aaaaccatct gaccataacg ccagctgccg
    13681 gggaaaggaa ggattatctg cggtttttgt gatgaaaagc aggatgatta tgtttaaccc
    13741 agtaaatacg ttcctgtctt tttttgtata aaaaatgttt attactcgat gtaaggatac
    13801 attaatttta cgtttattat gatgaatcct ctgcaaatga gcgaaatttg atagaacatt
    13861 tatctgctgt acgatttaat gaacttaatg atacagcatt acaaaaacaa tcgaagttta
    13921 taaagatgat tcctgattga ccagaaccct atcctacaat ggtgactaat tttattactc
    13981 ccga
//
LOCUS       DAZKIV010000124        13689 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZKIV010000124 DAZKIV010000000
VERSION     DAZKIV010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.124"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            104..4066
                     /gene="putA"
                     /locus_tag="ACHOT6_003557"
     CDS             104..4066
                     /gene="putA"
                     /locus_tag="ACHOT6_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9948716.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGYVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNATLMTIG
                     "
     gene            complement(4106..4744)
                     /gene="rutR"
                     /locus_tag="ACHOT6_003558"
     CDS             complement(4106..4744)
                     /gene="rutR"
                     /locus_tag="ACHOT6_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFP9948717.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            5032..6123
                     /gene="rutA"
                     /locus_tag="ACHOT6_003559"
     CDS             5032..6123
                     /gene="rutA"
                     /locus_tag="ACHOT6_003559"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFP9948718.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            6123..6815
                     /gene="rutB"
                     /locus_tag="ACHOT6_003560"
     CDS             6123..6815
                     /gene="rutB"
                     /locus_tag="ACHOT6_003560"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFP9948719.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            6827..7213
                     /gene="rutC"
                     /locus_tag="ACHOT6_003561"
     CDS             6827..7213
                     /gene="rutC"
                     /locus_tag="ACHOT6_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFP9948720.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            7221..8021
                     /gene="rutD"
                     /locus_tag="ACHOT6_003562"
     CDS             7221..8021
                     /gene="rutD"
                     /locus_tag="ACHOT6_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFP9948721.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            8031..8621
                     /gene="rutE"
                     /locus_tag="ACHOT6_003563"
     CDS             8031..8621
                     /gene="rutE"
                     /locus_tag="ACHOT6_003563"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFP9948722.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLREIYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPQLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            8632..9126
                     /gene="rutF"
                     /locus_tag="ACHOT6_003564"
     CDS             8632..9126
                     /gene="rutF"
                     /locus_tag="ACHOT6_003564"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFP9948723.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            9147..10475
                     /gene="rutG"
                     /locus_tag="ACHOT6_003565"
     CDS             9147..10475
                     /gene="rutG"
                     /locus_tag="ACHOT6_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFP9948724.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(10558..10731)
                     /gene="ymdF"
                     /locus_tag="ACHOT6_003566"
     CDS             complement(10558..10731)
                     /gene="ymdF"
                     /locus_tag="ACHOT6_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFP9948725.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            11104..11700
                     /gene="wrbA"
                     /locus_tag="ACHOT6_003567"
     CDS             11104..11700
                     /gene="wrbA"
                     /locus_tag="ACHOT6_003567"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFP9948726.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            11721..11948
                     /gene="yccJ"
                     /locus_tag="ACHOT6_003568"
     CDS             11721..11948
                     /gene="yccJ"
                     /locus_tag="ACHOT6_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFP9948727.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(11986..13227)
                     /gene="agp"
                     /locus_tag="ACHOT6_003569"
     CDS             complement(11986..13227)
                     /gene="agp"
                     /locus_tag="ACHOT6_003569"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFP9948728.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTRAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(13520..>13689)
                     /locus_tag="ACHOT6_003570"
     CDS             complement(13520..>13689)
                     /locus_tag="ACHOT6_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948729.1"
                     /translation="GNESFSTDDDLEYGYLINAGNHYDVYLPPELFAQAYKLKNKEMN
                     AQLDYLNRYAI"
BASE COUNT         3061 a         3611 c         3743 g         3274 t
ORIGIN      
        1 ttgctacgca ggtcacattt aacatggttg cacaaagttg caacatcatg gatatttcac
       61 gataacgtta agttgcacct ttcagaacaa caggagtaat ggcatgggaa ccaccaccat
      121 gggggttaag ctggacgacg cgacgcgtga gcgtattaag tctgccgcga cacgtatcga
      181 tcgcacgcca cactggttaa ttaagcaggc gattttttct tatctcgaac aactggaaaa
      241 cagcgatact ctgccagagc tacctgcgct gctttctggc gctgccaatg agagcgatga
      301 agcaccgact ccggcagagg aaccacacca gccattcctc gactttgccg agcaaatttt
      361 gccccagtcg gtttcccgcg ccgcgatcac cgcggcctat cgtcgcccgg aaaccgaagc
      421 ggtttctatg ctgctggaac aagcccgcct gccgcagcca gttgctgaac aggcgcacaa
      481 actggcgtat caactggccg ataaactgcg taatcaaaaa aatgccagtg gtcgcgcagg
      541 tatggtccag gggttattgc aggagttttc gctgtcatcg caggaaggcg tggcgctgat
      601 gtgtctggcg gaagcgttgt tgcgtattcc cgacaaagcc acccgcgacg cgttaattcg
      661 cgacaaaatc agcaacggta actggcagtc acacattggt cgtagcccgt ccctgtttgt
      721 taatgccgct acctgggggc tgctgtttac tggcaaactg gtttccaccc ataacgaagc
      781 cagcctctcc cgctcgctga accgcattat cggtaaaagc ggtgaaccgc tgatccgcaa
      841 aggtgtggat atggcgatgc gcctgatggg cgagcagttc gtcactggcg aaaccatcgc
      901 ggaagcgtta gccaatgccc gcaagctgga agagaaaggt ttccgttact cttacgatat
      961 gctgggcgaa gccgcgctga ccgccgcaga tgcacaggcg tatatggttt cctatcagca
     1021 ggcgattcac gccattggta aagcatctaa cggtcgtggc atctatgaag gtccgggcat
     1081 ttcaatcaag ctgtcggcgc tgcatccgcg ttacagccgc gcccagtatg accgggtaat
     1141 ggaagagctt tacccacgtc tgaaatcact caccctgctg gcgcgtcagt atgatattgg
     1201 tattaacatc gatgccgaag aggccgatcg tctggagatc tccctcgatc tgctggaaaa
     1261 actctgtttc gagccggaac tggcaggctg gaacggcatc ggttttgtta ttcaggctta
     1321 tcaaaaacgc tgcccgttgg tgattgatta cctgattgat ctcgccaccc gtagccgtcg
     1381 ccgtctgatg attcgtctgg tgaaaggcgc gtactgggat agtgaaatta agcgtgcgca
     1441 gatggacggc cttgaaggtt atccggttta tacccgcaag gtgtataccg acgtatctta
     1501 tctcgcctgt gcgaaaaaac tgctggcggt gccgaatcta atctacccgc agttcgcgac
     1561 gcacaacgcc catacgctgg cagcgattta tcaactggca ggtcagaact actatccggg
     1621 tcagtacgag ttccagtgcc tgcatggtat gggcgagcca ctgtacgagc aggtcaccgg
     1681 gaaagttgcc gacggtaaac ttaaccgacc gtgtcgtatt tatgccccgg ttggcacaca
     1741 tgaaacgctg ctggcgtatc tggtgcgtcg cctgctggaa aacggtgcta acacctcgtt
     1801 tgttaaccgt attgccgaca cctctttgcc actggatgaa ctggtcgccg atccggtcac
     1861 tgctgtagaa aaactggcgc aacaggaagg gcaaactgga ttaccgcatc cgaaaattcc
     1921 cctgccgcgc gatctttacg gtcacgggcg cgacaactcg gcagggctgg atctcgctaa
     1981 cgaacaccgt ctggcctcgc tctcctctgc cctgctcaat agtgcactgc aaaaatggca
     2041 ggccttgcca atgctggaac aaccggtagc ggcaggtgag atgtcgcccg ttattaaccc
     2101 tgcggaaccg aaagatattg tgggctatgt gcgtgaagcc acgccgcgtg aagtagaaca
     2161 ggcgctggaa agtgcggtta ataacgcgcc aatctggttt gccactcctc cggctgaacg
     2221 cgcggcgatt ttgcatcgcg ctgccgtgct aatggaaagc cagatgcagc aactgattgg
     2281 tattctggtg cgtgaagccg gaaaaacctt cagtaacgcc attgccgaag tgcgcgaagc
     2341 ggtcgatttt ctccactact acgccggaca ggtgcgggat gatttcgcta acgaaaccca
     2401 ccgtccatta gggcctgtgg tgtgtatcag cccgtggaat ttcccgctgg cgattttcac
     2461 cgggcagatc gctgccgcac tggcggcagg taacagcgtg ctggcaaaac cggcagaaca
     2521 aacgccgctg attgccgcgc aagggatcgc cattttgctg gaagcgggtg taccccctgg
     2581 cgtggtgcaa ttgctgccag gtcaggggga aaccgtgggc gcgcaactga cgggtgatga
     2641 tcgcgtgcgc ggggtgatgt ttaccggttc aaccgaagtc gctacgttac tgcagcgcaa
     2701 tatcgccagc cgcctggacg ctcagggtcg ccctattccg ctcatcgctg aaaccggcgg
     2761 catgaacgcg atgattgtcg attcttcagc actgaccgaa caagtcgtcg tggatgtgct
     2821 ggcctcggcg ttcgacagtg cgggtcagcg ttgttcggca ctgcgcgtgc tgtgcctgca
     2881 agatgagatt gccgaccaca cgttgaaaat gctgcgcggt gcaatggccg aatgccggat
     2941 gggtaatccg ggtcgcctga ccaccgatat cggtccggtg attgatagcg aagcgaaagc
     3001 caatattgag cgccatattc agaccatgcg tagcaaaggc cgtccggtgt tccaggcggt
     3061 gcgggaaaac agcgaagatg cccgtgaatg gcaaagcggc acctttgtcg ccccgacgct
     3121 gatcgaactg gatgactttg ccgaattgca aaaagaggtc tttggtccgg tgctgcatgt
     3181 ggtgcgttac aaccgtaacc agctaccaga gctgatcgag cagattaacg cttccggcta
     3241 tggtctgacg cttggtgtcc atacgcgtat tgatgaaacc atcgcccagg tcactggctc
     3301 ggcccatgtt ggtaacctgt atgttaaccg taatatggtg ggcgcagtgg tgggtgtgca
     3361 gccattcggc ggcgaagggt tgtccggtac cgggccgaaa gcaggcggtc cgctctatct
     3421 ctaccgtctg ctggcgaatc gcccggaaag tgcgctggca gtgacgctcg cgcgtcagga
     3481 tgcagagtat ccggtcgatg cacagttgaa agccgcattg actcagccgc taaatgcact
     3541 gcgggaatgg gcagcaaatc gtccagaatt gcaggcgtta tgtacgcaat atggcgagct
     3601 ggcgcaggca ggaacacaac gattgctgcc ggggccgacg ggtgaacgca acacctggac
     3661 gctgctgccg cgtgagcgcg tgttgtgtat tgccgatgat gagcaggatg cgctgactca
     3721 gctcgccgcc gtgctggcgg tgggcagcca ggtactgtgg ccggatgacg cgctgcatcg
     3781 tcagctagta aaggcattgc catcggcagt cagcgaacgt attcaactgg cgaaagcgga
     3841 aaatataacc gctcaaccct ttgatgcggt gatcttccac ggtgattcgg atcagcttcg
     3901 cgcattgtgt gaagccgttg ctgcgcggga tggcgcaatt gtttcggtgc agggttttgc
     3961 ccgtggcgaa agcaatatcc ttctggaacg gctgtatatc gaacgttcgc tgagtgtgaa
     4021 taccgctgcc gctggcggta acgccacttt aatgacgatc ggttaaacag gtagccggag
     4081 gatgttacaa cctcctccgg catctttaac gtggtcgaat cccctcaata ataatccgct
     4141 gcacgttttc aaccgtttga ttgaaaaata tctcatcgcg caacgtcgcg cctgtcaccg
     4201 cctccacctg aggggcgaaa tcggcgtaat gttgagtgga agcccaaatc atgaaaatca
     4261 aatgctgcgg atcaatcggc gcgagtttgc cgcttttgac ccaaccggca atcagcgccg
     4321 atttctcatc aattaatgcc ttcaaatcgc ccgttagttc atccattaac agcggcgcgc
     4381 ctgccagcat ctccatacag aacaggcgcg aagcctgcgg ataatcgcgg gagacttcca
     4441 gcttcagacg gatgtactct tttatcgccg ccagcggagc gaaatcttca cgaaacgctt
     4501 ttaacggtgc cagccagata tcgagaatct gccgcagcac ggcaatatac agcgcctctt
     4561 ttgacggaaa gtaatacagc agattggttt ttgaaacacc cgccaactct gcgatctgct
     4621 ccagccttgt gccgtgaaaa ccgaattgtg aaaaagtgtc cagtgctgcg ctaagaatcg
     4681 cttttttctt cgcgcttact gcgcgcgaac gtttacccgt tgttttcact gcgccttgcg
     4741 tcatgcgctc tcccctcttg tagattggca acaggatagc aaaacccccg cccggcctcg
     4801 accttctgca ctctcatcgc gcacagatgc atgttttatg tgcaactgtt ttgaccgttt
     4861 agtccacttt ttaccagata ttttagataa cgcattgtta acatattaac aacattaact
     4921 ttttaaaaac tggcatccgc tttgcaaaca agccattacg cggctgaaaa ggatatgcag
     4981 gatgcagcgc cccgtctgac tttcacttta cgagacgaag agaggttgat gatgaaaatt
     5041 ggcgtattcg tacctattgg caacaacggc tggctcattt cgacccacgc gccgcagtac
     5101 atgccgacct ttgaactgaa taaagccatt gtgcaaaaag cggagcacta ccatttcgat
     5161 ttcgccctgt cgatgatcaa actgcgtggc tttggcggca aaactgagtt ctgggatcac
     5221 aaccttgagt cgttcacctt gatggcgggg ctggcggccg tcacctcgcg cattcagatt
     5281 tacgccactg ctgccacctt aacgttacct ccggcaatcg tcgcccgtat ggccgcaacc
     5341 atcgactcca tctctggcgg gcgttttggc gtcaacctcg tgactggctg gcaaaagccc
     5401 gagtatgagc agatgggtat ctggcctggc gatgactatt tctcccgtcg ttacgactat
     5461 ctcaccgaat atgttcaggt gctgcgcgac ctgtggggca cggggaaaag cgattttaaa
     5521 ggcgattttt tcaccatgaa tgattgtcgc gtcagtccgc aaccgagtgt ccccatgaaa
     5581 gtgatctgcg ccgggcaaag cgacgctggc atggcgttct ccgcccagta tgccgatttc
     5641 aacttctgtt tcggcaaagg cgtaaatacg cccacggctt tcgccccgac cgctgcgcgg
     5701 atgaaacagg ccgcagagca aaccgggcgc gacgttggtt cttatgtgtt gtttatggtg
     5761 attgccgatg aaaccgacga tgccgctcgc gccaaatggg aacactacaa agcgggcgcg
     5821 gatgaagagg cgttaagctg gctaaccgaa caaagtcaga aagatacccg ctccggtact
     5881 gataccaacg tccgtcagat ggccgatccc acttcggcgg taaacatcaa tatggggacg
     5941 ttagtcggtt cttacgccag tgtcgcgcgc atgttagatg aagtcgcaag cgtgcctggt
     6001 gccgaaggcg tgctgttgac cttcgatgat tttctgtcgg gaatcgaaac cttcggcgag
     6061 cgcattcaac cactgatgca gtgccgcgcc catctccctg cgctgactca ggaggtggca
     6121 tgatgacaac cttaaccgct cgaccggaag ccattacctt cgatccgcag caaagtgcgc
     6181 tgatcgtggt ggatatgcaa aacgcttatg ccacgccagg cggctactta gatctcgccg
     6241 ggtttgatgt ctcaaccact cgcccggtca ttgccaacat tcaaaccgcc gtgaccgcag
     6301 cgcgagcggc agggatgctg atcatctggt ttcaaaatgg ctgggatgaa cagtatgtcg
     6361 aagctggcgg acccggctca ccgaattttc ataaatcgaa cgccctgaaa accatgcgta
     6421 agcagccgca gctgcagggg aaattgctgg cgaaaggctc ctgggattat caactggtgg
     6481 atgaactggt gccgcagcct ggcgatattg tgctgccgaa gccgcgctac agcggtttct
     6541 tcaatacacc gctggacagc attttgcgca gccgtggaat acgccatctg gttttcaccg
     6601 gtatcgctac caacgtctgc gtcgaatcga cgctacgcga tggctttttt ctggagtatt
     6661 tcggcgtggt gctggaagac gcaacacacc aggcggggcc ggaatttgca cagaaagccg
     6721 cgttgttcaa tatcgaaaca ttttttggct gggtcagcga cgtcgaaacg ttctgcgacg
     6781 cgctttctcc cacgtccttt gctcgtatcg cttaaggagt ttaacgatgc caaaatccgt
     6841 aattattccc gctggcagta gcgcaccgct ggcccccttc gttcccggca cgctggctga
     6901 tggcgtggtg tatgtctccg gtacgctggc ttttgatcaa cataataacg tgctgtttgc
     6961 cgatgaccca aaggcgcaaa cccgccacgt tctggaaact atccgcaagg tgatcgagac
     7021 ggcgggtggc acgatggcgg atgtgacctt caacagcatc tttattaccg actggaaaaa
     7081 ttacgccgcg attaacgaaa tctacgccga gttttttccg ggtgataaac cggcgcgatt
     7141 ctgcattcag tgcggactgg taaaacctga cgcgctggtg gaaatcgcca caattgcgca
     7201 tatcgccaag tgaggccgcg atgaaacttt cactctcacc tcccccttat gctgatgcgc
     7261 ccgtagtggt gttgatttcg ggtcttggcg gtagcggcag ttactggtta ccgcaactgg
     7321 cggtgctgga gcaggagtat caggtagtct gttacgacca gcgcggcacc ggcaataatc
     7381 ccgacacact ggcagaagat tacagtatcg cccagatggc agcggaactg catcaggcgc
     7441 tggtagccgc agggattgag cattacgcgg tggtcggcca tgcgctcggt gcgctggtgg
     7501 gaatgcagct ggcgctggat tatcccgcgt cggtaactgt gctcatcagc gttaacggct
     7561 ggctacgaat aaacgcccat acgcgccgct gttttcaggt tcgcgaacga ttactgtata
     7621 gcggcggcgc gcaggcatgg gtggaagcgc agccgttgtt cctctatccc gccgactgga
     7681 tggcggcccg cgcacctcgc ctggaggcag aagacgcgct ggcactggcg cattttcagg
     7741 gcaaaaataa tttactgcgt cgacttaacg ccctcaaacg cgctgacttt agtcaccatg
     7801 cggatcgcat ccgctgcccg gtgcaaatca tctgcgccag tgatgatctg ctggtgccaa
     7861 cagcatgttc cagtgaactt catgccgccc tgcccgatag ccagaaaatg gtgatgccct
     7921 atggcggaca cgcctgcaac gtgaccgatc ccgaaacgtt taatgctctg ttactcaacg
     7981 ggcttgccag cctgttacat caccgtgaag ccgccctgta aggaattgct atgaacgaag
     8041 ccgttagccc aggtgcgctt agcaccttgt tcaccgatgc ccgcactcac aacggctggc
     8101 gggagacacc cgtcagcgat gagacgttac gggagattta tgccctgatg aaatgggggc
     8161 cgacatcagc taactgttct ccggcacgga tcgtgtttat ccgcacggca gaaggaaaag
     8221 aacgtctgcg cccggcactt tccagcggca atctgcaaaa aaccctgacc gcgcccgtca
     8281 ccgctatcgt cgcctgggac agtgaatttt atgaacggtt accgcaactg tttcctcacg
     8341 gcgatgcccg cagttggttt acctccagcc cacaacttgc cgaagaaaca gcgtttcgca
     8401 acagttccat gcaggcggcc tatctaatcg tcgcctgccg tgcgctggga ctggataccg
     8461 gcccgatgtc gggttttgac cgtcaacacg ttgacgacgc cttttttgcg ggcagcacgc
     8521 tgaagagcaa tctgctgatt aatatcggct atggcgatag cagcaaactt tttgcgcgcc
     8581 tgccacgtct gtcctttgaa gaagcctgcg ggctgttgta aggagcaaac catgaacatt
     8641 gtcgatcaac aaacctttcg cgatgcgatg tcctgtatgg gcgcggcagt caatatcatc
     8701 accacggatg gtccagccgg gcgcgccggg ttcaccgcca gcgcggtctg cagcgtgacc
     8761 gacacgccgc ctacattact ggtgtgcctg aatcgtgggg cgtccgtctg gccggtattc
     8821 aatgaaaatc gaacgctgtg tgtaaataca ctgagcgccg ggcaggagcc gctttcaaat
     8881 ctttttggcg gcaaaacgcc catggaacac cgctttgccg ccgcccgctg gcagacgggt
     8941 gtgaccggat gcccgcaact ggaagaggct ctggtttcgt ttgactgccg tatcagccag
     9001 gtggtgagcg tcggcaccca cgacattctg ttttgcgcca tcgaagcgat tcatcgtcac
     9061 gccacaccct acgggctggt gtggttcgat cgcagttatc acgcgctgat gcgccctgct
     9121 tgttaacctc cttaaggaga cagctgatgg caatgttcgg ttttcctcac tggcagttaa
     9181 aatcgacctc tacagagagc ggcgtggtcg cgccgtatga acgattgccg tttgctcaga
     9241 cggcagtaat gggcgttcaa cacgcggtgg cgatgtttgg cgcgacggtg ttaatgccga
     9301 ttctgatggg gctggatccc aatctttcca ttttaatgtc ggggatcggc acgttactgt
     9361 ttttctttat taccggcggg cgcgtcccca gttatctcgg ttccagcgcg gcttttgtcg
     9421 gcgtggtgat tgccgcgacg ggctttaacg gtcagggcat taacccgaat atcagcattg
     9481 cgctgggcgg gattatcgcc tgtgggctgg tttataccgt cattggcctg gtggtgatga
     9541 aaattggtac acgctggatt gaacgactaa tgccgccggt ggtgacgggt gcggtggtga
     9601 tggcgattgg cctgaactta gcgcctatcg cagtgaaaag tgtatcggct tcggcctttg
     9661 atagctggat ggcggtaatg acggtgctgt gtattgggct ggtggcggtg tttacccgtg
     9721 gcatgatcca gcggttattg attctcgtag gtttaattgt cgcctgtctg ctgtatggcg
     9781 tgatgaccaa cgttttaggg ctgggaaaag ccgtcgattt tactctcgtc agccatgctg
     9841 cctggttcgg tttgccacat ttttccactc ctgcttttaa tagtcaggca atgatgctga
     9901 ttgcgcccgt ggcggtgatt ctggtggcgg aaaacttagg tcatctcaag gcggtcgccg
     9961 ggatgaccgg acgcaatatg gacccgtata tggggcgggc attcgtcggt gatgggctgg
    10021 cgacgatgct ttctggttct gtcggtggca gcggcgtaac cacctacgcc gagaatatcg
    10081 gtgtaatggc cgtgacaaaa gtctactcga cgctggtgtt tgtcgctgct gccgtcatcg
    10141 ccatgctgtt gggcttttca ccgaaatttg gcgcgctgat tcataccatt ccggccgcgg
    10201 tgattggtgg tgcatcaatt gttgtgttcg ggctgattgc cgtcgcaggc gcaaggatat
    10261 gggtacaaaa ccgtgtcgat ttaagccaga acggtaattt gattatggtc gcagtaacct
    10321 tagtgctggg cgcgggtgat tttgctctca cgctgggcgg ttttacgttg ggagggattg
    10381 gtacagcaac ctttggcgcg attttactca atgcgttgtt aagcagaaag ttggttgacg
    10441 ttccaccgcc agaagttgtt catcaggagc cataaacaga aaaccgccgg gattaccggc
    10501 ggtgatgact ttttgacgag cctaagaaaa aaatgcattc gtcattgatt agcccggcta
    10561 gttgtcgctt ttgccgtgac tgctcttgcc acctttttta cctgcttcag atgcgcgctg
    10621 cgggtcattc ttgaaattac ccccgctgtg ctgtccacct tttttacctg cttctgatgc
    10681 tctttcgcgg tcttctgcaa aattgccgga accgcctcga tggtttgcca ttactgacct
    10741 ccgtctgggt atagacatca tattgcatag aattaacgcg gcgtatggcg gctgccatat
    10801 tttcgctacg tgggactaag cttagtgaac tgacgcgggc agacccgcgt ttggtaatat
    10861 cctgcaacag cttcttagcg agaatagata tcatctcaaa taattatcca taagccgctc
    10921 ttatgattaa aaaaacgcga tgagataaaa atgtgtcatt ttgcgacaaa attacgtgct
    10981 tgtgaaagtt gttataaatc aaataagtgg ttgtgaaatt tgcactctga aaaggacgtc
    11041 ttatctttaa ataagtggta gcgaatcgct acggaataga gataacacga ggagtggtta
    11101 gaaatggcta aagttctggt gctttattat tccatgtacg gacatattga aacgatggca
    11161 cgcgcagtcg ctgagggtgc aagcaaagtc gatggcgcag aagttgtcgt taagcgtgta
    11221 ccggaaacca tgccgccgca attatttgaa aaagcaggcg gtaaaacgca aactgcaccg
    11281 gttgcaaccc cgcaagaact ggccgattac gacgccatta tttttggtac acctacccgc
    11341 tttggcaaca tgtccggtca aatgcgtacc ttcctcgacc agacgggcgg cctgtgggct
    11401 tccggcgcac tatacggaaa actggcgagc gtctttagtt ccaccggtac tggcggcggt
    11461 caggaacaaa ctattacttc aacctggacg acccttgcgc atcacggcat ggtaattgtc
    11521 cccattggct acgcagcgca ggaattattt gacgtttcac aggttcgcgg cggtacgccg
    11581 tacggcgcaa ccaccatcgc aggcggtgac ggctcacgcc agccaagcca ggaagaactg
    11641 tctattgctc gttatcaagg ggaatatgtc gcaggtctgg cagttaaact taacggctaa
    11701 tcttcaacag gaggatacgc atgccaactc aagaagcgaa agctcatcac gtcggtgaat
    11761 gggcatctct gcgcaatacg tcgccggaaa tagccgaggc cattttcgaa gtcgccgggt
    11821 acgacgaaaa aatggcggaa aagatttggg aagaaggtag cgatgaagtg ttagtcaaag
    11881 cctttgctaa gaccgataaa gattcgcttt tttggggcga acagaccatc gaacgtaaaa
    11941 acgtttaacc agcgactccc ccacttctcg cgggggagtt ttctgttatt tcaccgcctc
    12001 attcaacacg ctatcaaact tatccattgg gcagaaaccg ttggcatcta tcgggcaacc
    12061 gcttaattcc agcgtcacgc gctgcgcagg tgcctgcagg gttaacgcat cggcattacg
    12121 taactgttcc gcactctggt acacatattc aattttcatc aaatcgcgat tggctttgct
    12181 gtcatgccaa cgctggaaaa cgattttgcc gccaatcggc gtgcgttcgt tctggtcatg
    12241 caactgatac ggtttgaaat ccagcgccgt taacagagag gctatgttgg agtcgtgccc
    12301 aactaacacc gtgattttcg gtgcacgggt gcgatcggtg acaagggctt tgtcgatata
    12361 actgaccagc ggtttcgcaa cattgcgcgc cacttccggt gaggtaaaca ggctgtcctg
    12421 gtaaccgttt ttcagcttcg acaatacctt ccactgctgg tcagatttga tttctcccca
    12481 ggccacctga tccatcggaa aaccttcgta gtattgcaaa gtaaacgcat ctaccagcga
    12541 gttgccgact ttcagcggcc cggaaacacc tggttcttgt tgatacttcg cgctaaaggt
    12601 atttttgcca tccaccagcg aacactgttg tttctcttta caggcagggg aatctttata
    12661 gttaacgatt ttttccagta gctggtagct gtcggtaagc tggagtttgc tgagctcttt
    12721 ctccattgcc gccaccgcct gttcactgaa tgcggcggaa tcatcggtga tcaccgggtt
    12781 aaaggttggg tccatagtgc ccattttttc ctggtgatgc acaggaatat cacaccccgg
    12841 gaatgcgccg gtaataaaga actgtgcggt cgcaacggta cgttgcagac tattggcata
    12901 ggcataaacg gtgtacggcg gcgggcattc ccccgatttc accatcccct gctctgccag
    12961 ccattcacgc atgtaatggc ccatatacac ttcgagcacg ccacctttgg tggtgagttg
    13021 cccaccgggg acgtcccatt ctggccattt attcggcgtc gactgctcca gcacactgcc
    13081 attgttcgcc agcggcgcac gtaagttatg gcggctcatc atgagcactt gctgtagctg
    13141 atagccttcc ggtacggttt gtgcctgagc gtttgaagcg agtaaaacta tccctgccac
    13201 agctgcggcg attagcgttt tgttcattcc tggcacctct tttgttatca atcactaaag
    13261 agtgtgacag aaatatgact tttttccggg aacaggttcg caaaatggct tgttgaacgc
    13321 ctgtttttct atatacatct tcaaaaacag gcaaggtttg acgcaaacta tgcctgatta
    13381 gcccctgcct cgcgtacgcc gctgataatt gcacctgtgg ctaaacagaa tatctgcggc
    13441 gttaccaata cagagacaat atttaccaat aaaaaagtgt gagactaata acaatggttt
    13501 ttagcatatg cctttgccat taaattgcat aacgatttaa atagtcgagt tgcgcattca
    13561 tttccttatt ttttaactta taagcctgag caaaaagttc aggagggagg taaacatcat
    13621 aatgattgcc agcgtttatt aaataaccat actcaaggtc gtcatctgtt gaaaaggatt
    13681 cattcccca
//
LOCUS       DAZKIV010000125        13685 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZKIV010000125 DAZKIV010000000
VERSION     DAZKIV010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13685
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.125"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            91..921
                     /gene="yjiC"
                     /locus_tag="ACHOT6_003571"
     CDS             91..921
                     /gene="yjiC"
                     /locus_tag="ACHOT6_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFP9948730.1"
                     /translation="MSMPLSNALQSQIITDDHFLHHPIVDSELIRKYERARLDTENIY
                     LLPLVRGNNHNYDGKSVVEIRKLDISKEYWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TSALDDIYGGICKKAHAATELAEGLRLNLFMKSPSYPVENYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1062..1835)
                     /gene="uxuR"
                     /locus_tag="ACHOT6_003572"
     CDS             complement(1062..1835)
                     /gene="uxuR"
                     /locus_tag="ACHOT6_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFP9948731.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2050..3510)
                     /gene="uxuB"
                     /locus_tag="ACHOT6_003573"
     CDS             complement(2050..3510)
                     /gene="uxuB"
                     /locus_tag="ACHOT6_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFP9948732.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPAYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3591..4775)
                     /gene="uxuA"
                     /locus_tag="ACHOT6_003574"
     CDS             complement(3591..4775)
                     /gene="uxuA"
                     /locus_tag="ACHOT6_003574"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFP9948733.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRVFF
                     SR"
     gene            5115..6458
                     /gene="gntP"
                     /locus_tag="ACHOT6_003575"
     CDS             5115..6458
                     /gene="gntP"
                     /locus_tag="ACHOT6_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFP9948734.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6701..7603)
                     /gene="fimH"
                     /locus_tag="ACHOT6_003576"
     CDS             complement(6701..7603)
                     /gene="fimH"
                     /locus_tag="ACHOT6_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9948735.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7623..8066)
                     /locus_tag="ACHOT6_003577"
     CDS             complement(7623..8066)
                     /locus_tag="ACHOT6_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948736.1"
                     /translation="MQAADVTITVNGKVVAKPCTVSTTNATVDLGDLYSFSLMSAGAA
                     SAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQGTAQNIQLELQDDSGNTLNTG
                     ATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVISITYTYS"
     gene            complement(8139..8669)
                     /gene="fimF"
                     /locus_tag="ACHOT6_003578"
     CDS             complement(8139..8669)
                     /gene="fimF"
                     /locus_tag="ACHOT6_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFP9948737.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8679..11315)
                     /gene="fimD"
                     /locus_tag="ACHOT6_003579"
     CDS             complement(8679..11315)
                     /gene="fimD"
                     /locus_tag="ACHOT6_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFP9948738.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHHLAGFFVRLFVACAFVAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNMASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRILYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNETVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11382..12107)
                     /gene="fimC"
                     /locus_tag="ACHOT6_003580"
     CDS             complement(11382..12107)
                     /gene="fimC"
                     /locus_tag="ACHOT6_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9948739.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12144..12683)
                     /gene="fimI"
                     /locus_tag="ACHOT6_003581"
     CDS             complement(12144..12683)
                     /gene="fimI"
                     /locus_tag="ACHOT6_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9948740.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGVANAQAWFSLTYQ"
     gene            complement(12748..13296)
                     /gene="fimA"
                     /locus_tag="ACHOT6_003582"
     CDS             complement(12748..13296)
                     /gene="fimA"
                     /locus_tag="ACHOT6_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9948741.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALALDGATFSSETTLKNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3339 a         3512 c         3300 g         3534 t
ORIGIN      
        1 tgcatgtcat ttgtttactt ctctttttac tacagaaaaa tttatctatt tttctaaaca
       61 tcagtgaaat actcataaaa ggtaactatt atgtctatgc cattaagcaa tgcccttcag
      121 agtcaaatta ttacagatga tcacttcctt catcacccaa tagtcgacag tgagttaata
      181 cgtaaatatg aacgtgcacg cctggatacg gaaaatatct atcttctgcc tcttgttcgt
      241 ggcaataacc ataattacga tggaaaaagc gttgtcgaaa ttagaaagct tgatattagt
      301 aaagagtact ggccatttaa ttatgtgaca gaaacatgca gagaatccga tggtattaca
      361 acaacaggac gtatgctcta tcgaaacttg aaaatcacct cagctttaga cgatatatat
      421 ggtggtatat gtaaaaaggc gcatgctgcc acagagttag cagaaggatt acggctaaat
      481 ttatttatga agagcccttc ctatcctgtt gagaactata cagttcatga aataacatta
      541 ggccctggtt gcaatgttcc aggttacgcc ggaaccacga taggatatat ttcaaccctt
      601 ccagcttctc aggcaaaaag atggaccaat gagcagccaa ggattgatat ctatattgat
      661 cagataatga ctgtctcagg ggtggcaaat tcttccggat tcgcccttgc cgcattactt
      721 aatgctaata ttgagttggg taatgaccca ataattggta tagaagccta tcccggtacc
      781 gcggagatcc atgcaaaaat gggatataaa gttatcccgg gtgatgaaaa tgcaccactc
      841 aaacgtatga ctctgcaacc gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa
      901 tggaattata ttggtaaata gccactgaga agaatgagta ctaacattca ggttatacag
      961 atagctaaac aataccctgc aatttacaaa gtaatactca ttgcgacttc ttaatttaag
     1021 aaagcgcaag gaacgtttac ccttgcgctt attataataa gtcaggcgtc gactttatcc
     1081 agcggccatg aatcaaacag atagccatca aaataaatat cgtcaacgtt cgagaattcc
     1141 agcagacgtt gcttaacgtt ttccagatgc tgccacattg ccagcttcgc cgctcgggca
     1201 tcttttttga ttaacgcggc gaggatctgt ttgtgatcgc ccaaccactc tttgcgatac
     1261 aggctgtcat ccagatggct gtgcaactgg atccacattg gattgttttc ccgccactgc
     1321 caggactgac ggaacagctc caccagcatg ctgttatgcg ttgcttcggc aatagcgaga
     1381 tggaactgca tgtcaccgct ttcgctgctg cccggcgcac tggaagccag ttcacgctct
     1441 tccagttgca atgcctgacg cattttgacg atatcttcgc gggtagcctg caaagcggca
     1501 aactcggcga tgttgctctc caataactgc cgcgcctgta acagctcaaa aggaccggca
     1561 tcgttgcaga cgttggcatc cggactgtct gtgttctggc tgcctgagtt atcaagaaca
     1621 tagataccgg caccccggcg tacttccacc agccctttga tctccagcat gatcagcgct
     1681 tcacgtacca ccgtccgcgt gacatcaagc atttctgcaa tttcacgctc cggcggcagc
     1741 cgttcgccag ggttgtacgg cgtctttatg atcagatcgc ggatcatcgc cccgacttcc
     1801 tggtaaggtc tttgcgcaga ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt
     1861 aatcaaattt ttgccggatg cggcgtgaac gccttatccg gcctacaacg ggcaatttta
     1921 cggcccccgt taatctcaaa ctataccatt agcactggaa gttggcacgt acaggtgggc
     1981 aaaaaaacga aaactggtca accaaatatc agtttgattg accagtaagt ctgtagggta
     2041 atcagttagt tacagcgcag ccacacactc gcgcgcaccg cgttcgcaca gctgctgata
     2101 tgccgccgtc actgcgccaa caaagtcggc attctgcggc agatcatcgg caaaaatacc
     2161 gctcaggccc agcagcgctt tcacgcggtc tgcgccctga tactgcgcgt tgatcttctg
     2221 gaactccgcc agcatcgggt cgaccacgtc aatggcatta ccctgctcat ccacgccctg
     2281 ggtgtaacgc atccagccag ccacgcccag cgccaggtga cgccagctgc cgccgttttg
     2341 caggtgcaga cgcaccgggt ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc
     2401 aatctgccag gtacggtgac gcagagacgg gttgctgaaa cgctcgatca gcaacgtcgc
     2461 ataggcgttc aggtccgtac cttccggcat cgacagcgtt ggcgcttgtt cctgcatcat
     2521 cagggagaag gccgctttgc gataagccgg gttagtcatg gtgtcggcaa tggtttcata
     2581 gccgccgagg taaccgaggt acgccagaaa agagtggctg ccgttcagca tacgcagctt
     2641 catcatttcg aacggcacaa cgtctgcaac gaactgtgcg cccactttat cccagtccgg
     2701 gcgaccatta acgaagttat cttcaatcac ccactgacgg aacggttcgc aggcaatggc
     2761 gcacgggtcg taaacaccca gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc
     2821 cggaacgatg cggtcaacca tggtgcacgg gaaggtgaca ttttcttcaa tccatgccgc
     2881 cagctgcggg tcacgcgcct gagccagccc cagtaccgcg accttcgcca catgaccgtt
     2941 ttcacgcacg ttatcgcagg acatcaccgt aaacgctttc aacccttttt cacgacgcag
     3001 acgcaaggct tcgacgatgt aaccaatcgc ggacttcggc gcagtcgggt tttccagatc
     3061 gtgcttgatc agcgggttat tgagatccag ttgaccgctt gccgcatcag cgcagtagcc
     3121 tttttccgtg accgttagag agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat
     3181 accttcgcag ccatcgattt ccggatgcag cgcttctttc atcgaaccga taattttcag
     3241 ctcggtgctc tctgcgcctt tttccgctac ggtgtacagc agttgctgtt ttttcaggtt
     3301 ttcgatcagc acgcggtcgt tgcctggcat caggttaact tcgcagatgc cccagtcgct
     3361 gtcggtgctt tccagcagat gatgggtata cagcgcctgg tgcgcgcggt gaaacgcccc
     3421 gcaaccgaga tgcacaatgc gtgattccag acgagaatga tcccatgacg ggcgggcaac
     3481 cggcagattg ctgtcaacaa tagtagtcat cgtaaactcc agaatccgtc ttcaaagagt
     3541 gacggcaagg aagaaggaac cggcacggcg cgcagccatg ccggtggata ttaacggcta
     3601 aagaaaacgc gctggatcgc cagttcgaca ccgcgaactt cggccaggcc tttcagacga
     3661 ccaattgcgg agtaacctgg gttggttttc ttcttcaggt cgtccagcat ctgatgaccg
     3721 tggtccggac gcatcgggat caggtcttct ttgccttccg ctttacgacg gtgttcttct
     3781 tcaacaatcg ctttcaccac ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg
     3841 tggaaggttt tcgggttatc ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc
     3901 ggaccgaact gcttgatcat atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg
     3961 gtgcacatgg taaaaccgtt tgccatgctg tttacggtat caaccatcca ctgcatatct
     4021 tcaatggtgg aaacaatgcg cggcaggccg aggatcgggc gcggcggatc gtccgggtga
     4081 acagccatac gcacgccgac ttcttcagca actggaataa tcgctttcag gaagacggca
     4141 aagttttcgc gcagcttcgc tttgtcgata tctttgtaca gctccaggtg tttacggaac
     4201 tggtcgaggg tgtagccttc ttccgcgccc ggaagaccag caatgatgtt acgggtcaga
     4261 cgcgctttgt cttcgtcgct catagtggcg aagcgaacag cagcctgagc aatttcttct
     4321 tcggtgtaat ccgcttccgc gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat
     4381 tcgatctggt cgaagcgcag agctttggag ccgtctggca gcacgtattc gaggtcagta
     4441 cgggtccagt cgagcaccgg catgaagttg tagcacacgg tgcgaatgcc acactgcgcc
     4501 aggttgcgca gagtctgctg atagttagcg atccactgct catagttgcc agtgtgggtt
     4561 ttgatatctt cgtgaattgg tacgctttct acgacagacc acaccaggcc tgcgtcttca
     4621 acgatcgcct tgcgtttgag gatctcttct acggaccata cttcgccgtt cgggatatgg
     4681 tgcagcgcgg taaccacgcc agttgcgccc gcctgacgga catcagctaa agaaaccgga
     4741 tcgtttgggc cgtaccagcg ccaggtctgt tccatgattt ttcctctttc acatagtggg
     4801 ttccacagca aagagacgcg gaaccctgga gttgtttagg tatccggatg aacaaccaga
     4861 ccgcagccgt ggcgttgcat cggatgagat ggcgtataag ttctaccttt tggtaagaca
     4921 tgtcaacccg aattctgaaa ttggttaacc acatcacaag aatttcactc tcctggttgt
     4981 tgctgcaaat tgcagcaaaa ggttatcgat ggttattgat cagaggatgt gacattcatc
     5041 gcaacaatgg ttgaccaatt cacataacat gtcgcccaaa ataacaacag ttcaaccaag
     5101 taaggtaaaa cgttatgcat gtgcttaaca ttctctgggt agtattcggt atcggcctga
     5161 tgctggtact gaatttgaag ttcaaaatca attcaatggt ggctttgttg gtggcggcgc
     5221 tgtccgtcgg gatgctggcg ggcatggatt tgatgtcgct gctgcacact atgaaagcgg
     5281 gcttcggcaa cacgctgggg gaactggcta tcatcgtggt gttcggtgcg gtcatcggta
     5341 aattgatggt cgactccggc gcggctcacc agatagcgca tacgctgctg gcgcgtctcg
     5401 gtctgcgcta tgtacagctg tcggtgatta tcatcggcct gatttttggt ctggcgatgt
     5461 tctatgaagt ggcctttatc atgttagcgc cgctggttat tgttattgcc gccgaagcta
     5521 aaattccgtt cctgaaactg gcgatcccgg cagtagcagc tgccactacc gcacattcac
     5581 tgttcccacc gcagccgggt ccggtggcgc tggtgaatgc ttatggcgcg gatatgggga
     5641 tggtttatat ctatggcgta ctggtgacga tcccaagtgt aatctgcgca ggtctgatcc
     5701 tgccgaagtt cctcggcaat cttgagcgcc caacgccatc attcctgaaa gcagatcaac
     5761 cggtagatat gaataatctg ccctctttcg gcgtttcgat tctggtgccg ctgatcccgg
     5821 cgatcattat gatctctacc accatcgcca atatctggct ggtaaaagat acccctgcct
     5881 gggaagtggt taactttatc ggttcctcgc cgattgcaat gtttattgcg atggtggttg
     5941 cattcgtact ctttggcacc gcacgtggtc atgacatgca gtgggtgatg aacgcttttg
     6001 aaagcgcggt gaagagtatt gcaatggtga ttctgatcat aggtgcgggt ggcgtgctga
     6061 agcagaccat catcgacacc ggcattggcg acaccatcgg catgttgatg tcccacggca
     6121 atatctcgcc ctacatcatg gcatggctga tcactgtgct aattcgtctg gcgacgggtc
     6181 agggtgtcgt ttcggcgatg accgccgccg ggattatcag tgctgcaatc ctagatccag
     6241 caactggtca gctggttggc gtgaatccgg cgctgctggt actggcgacg gctgcgggtt
     6301 ccaacaccct cacccacatt aacgatgcat ctttctggtt gttcaaaggt tactttgacc
     6361 tgtcagtaaa agacacgttg aaaacctggg gtctgctgga gctggtcaac tccgtggttg
     6421 ggctgattat tgtgctgatt attagcatgg tagcgtaaaa ggacaccaga gcctgccaat
     6481 ggcaggctca gactgatgac aaagtctaaa aatgtgcccg gacagtcccc tcgcccctcc
     6541 ggggagaggg ttagggtgag gggaacagac cagcaaagtg cgctttgttt atgccagatg
     6601 cgacgctgac gcgtcttatc tggcctacaa agggctaacg tgcaggtttt tagcttcagg
     6661 taatattgcg taccagcatt agcaatgtcc tctgatttct ttattgataa acaaaagtca
     6721 cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt gcataatttg
     6781 ccgttaatcc cagactcacc gccgaagtcc ctactgctcc taacgatacc gtgttattcg
     6841 ctggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgtgca ggtgaaaacg
     6901 acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatacc
     6961 ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt
     7021 ccggcagagt aacggtgaca tcacgagcag aaacatcgca gccgccagta ggcaccacca
     7081 catcattatt ggcgtaaata ttccacacaa actggaaatc atcgctgtta tagttgttgg
     7141 tctgtcgcaa aataagcacg gcaattaatg agccagcttt aatcgccacc ccgcccgcac
     7201 tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa
     7261 caacgcgcgg cgtttcgctg gtggtaggaa atggatagct actgccacta tattttacgg
     7321 tcccggaaaa attagataac acgccgccat aagccgagcc tcgttgcagt gtgacatagt
     7381 ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tccacgacca
     7441 ggttttgccc cacattcacg gcgggcgcaa ggtttacata aacattggcg ctgccaccgc
     7501 caatagggat agcggtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc
     7561 agcccatcag cagtacagca aacagggtaa taactcgttt cattacaatc atctcttcgg
     7621 gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg
     7681 ccatttactg tcaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc
     7741 acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc ctgtagctct
     7801 aactggatgt tttgcgcggt cccctggttt ttataatatc cggtactgtc ggctgccccg
     7861 ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc aagcgcaaca
     7921 tcatgccagg ccgatgccgc cccggcagac ataagactga aagaataaag atcgccgaga
     7981 tcaaccgtgg cattggtggt ggaaaccgta cacggtttgg cgacgacctt accgttcacc
     8041 gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaatat tgccgccaat
     8101 acatacccac gtttgcacca tttcatgagc atctccagtt actgatattc aagagtgaag
     8161 gtagccgtgg cattgatatg ccccgcagtg acaggcacct gtgtcgccat tagccgggcg
     8221 taaaaattca gcgtatttgg tttacccggc gtcagggtcg tccacgaaag cgcggacgat
     8281 ggagcattaa ggggtatttg attttgctgc tcattcagaa gctgtattcc cagtcccgaa
     8341 gccgctgaca ccgtattttc aagtgcaagc aggttggcat tgtggctatc tgcaacgcca
     8401 gtaaacccaa cctttacggc agaaacggca ttaccacagg gtgacagcaa aatacgaaat
     8461 ggaacaacag gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga
     8521 tcaacagtaa aattggttga ttcagcggcc acactacagc cgttatccct gacatagccg
     8581 cggatagtaa tcgtgctatc cgcagccaaa gcgtgactca ccgccagcca caaaaaagcg
     8641 cacagaagat aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc
     8701 tgggttaata actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca
     8761 ttttcctctt ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa
     8821 acctgaccat tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc
     8881 ccaaacggca gcggcttatt attgtgggtc agcgtcatga gcagttttat cccaacgcgc
     8941 gctttaaact ctgctcgcac gatcgcccca cgagtgggaa caacgttagc aaccgcgtta
     9001 tctaaatcga cgttatcagc cagggtattg gtatccagcg ccactctatt ttcccgatat
     9061 tcagtggcat aaggcagcac ggcataacca cgccagtcgg tacgcacccc cgtctggttt
     9121 tcgacttttg catcttttgc gccaggcgct ttaacaagca ccactgtttc gtttaacggc
     9181 tgccccagcg ttacgccatt ggcatgagcc aataccccac cgctgactcc gtaatagagc
     9241 tgcttaatat catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa
     9301 ttcagcgtgg cgtagcctgt acttccgcta tttccatcgc ctcccccggc atagccggtt
     9361 tgcacgctat agctgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg
     9421 gtcatccgac cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga
     9481 gatttactgt cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc
     9541 tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata
     9601 tcttcgaatg cagtatttaa accagcctgg aattgctcat cgacattact cgttccccaa
     9661 taagtttgat ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg
     9721 gtgagctgta atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc
     9781 ttaacctgaa taactccgtc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt
     9841 gtatcagcga aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc
     9901 gtgcctgact cattgagcga tttgttatag agaatacgca ccgattgtcc gtcatgctga
     9961 ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc
    10021 cccatgtttt tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca
    10081 ccatatattg tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt
    10141 ttttcctgct gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgt
    10201 ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg
    10261 tcagcctctt tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg
    10321 atggtaaaag gccccggtgg caccgtacta ttataaatgt catacccatt ttgtttaata
    10381 gtgacctgtg cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta
    10441 tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa
    10501 atatcgccct gagtataacc atcacccagc gtcagccggg aacgtaacgg tattatgtct
    10561 cgctcaagcc aggtattgat atgctgccat ttatttttgc tacctgatga tctgtcgcta
    10621 ctgttataac tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc
    10681 tgtaggttta aatatgcata atggctgtta cccccaatcc gattctgtac actatttccg
    10741 ctgaaattat aattgagcaa tccggcatta ataccgggat cccataactc aggaggaata
    10801 taaccacgcg cgcgattact cataaatgcc tgagggatcg tcaggttgag tcgctgctga
    10861 ccaacatcta aatgcgcagt agcgtcctgg accattgtgg ttaatggcac acaggcatca
    10921 tccgcgagga gattcatacc ggcgacagaa gccatattca gccccatact ggcgagttgc
    10981 gcgcgtgtca ggcagggaac aatcccttgt tcactgtcgc ccgtattaaa tgtgacatca
    11041 cgcgttgcca tataaccatt attcaaatag atatcgacgc gatacgtccc tggcggtaat
    11101 tcttgcccat tttcaaaacg cgataaatcg gccacagcct ggggatcatc cgctaaaaag
    11161 cgcgggttaa aatagagttc ggcagatgac aaaggtgcct gtgcgacaaa agcacaggca
    11221 acaaagagcc ggacaaaaaa accagccaaa tgatgcttac gaatatgcaa gcattgtgtg
    11281 tttcgctggt aaagtcttaa attcagatat gacattattc ctccggttta aaatcacccg
    11341 gcagtcaatt ctttttattc aggcgaaaaa ttccccctgc gttattccat tacgcccgtc
    11401 attttggggg taagtgcgcc ataatcattt attgttcggt aagtaatatt gcttcctgca
    11461 tcagaaggca atttaaccgt gctttcgccc attggaggca ccaatgcatt ttcaagaacc
    11521 cgggttccgg cattcaactc tgttaccgtc aggtaatagg gtgtcgggtt aatcagcgtc
    11581 agagaattcg cgctacgacg aaatcttaat ttttctgcgg cctgatcggg tggcaacgct
    11641 aatttagccg ggcgatagta caatttaatg cggctgataa ttgcgagctg tagcgtattc
    11701 tcagtcaatt ttgatttatc cattgacgga atcgctttaa cgttcatcca gaataaactt
    11761 tcccggtcct gtggcaattg gttatttgtt gcatcaagaa tacgcaaggt attctctttt
    11821 tttcccttca tcgcaaacag aggtggcgtc acgataaaac gaccatcctt tacaccatcg
    11881 gcattttcca cccatgattg aattaaatag gtgctatttt catcattatt tgtcacggca
    11941 agttgcactt gtttttgccc tgccggataa attacgcgag tcgcacctaa ggccactccc
    12001 gcttcggcgc gtccggcaac catcattgcc atgaacatca ggatacctgc cagcaagcag
    12061 aatgttattt cctgcgattt ccttacattg acgtttttat tactcactgt cctgttcctg
    12121 ttatcacatt atctgctgaa caattactga taggttaaag agaaccaggc ctgggcattg
    12181 gcgacgccgc cagtaacccg acgcccggta gcacgatatt tggcgatgaa atgtagcgaa
    12241 gtagagcctg agtaaagccg tttccagttt gctggaggac gattaatcgg tacgaggttt
    12301 ccttcatcat caaacaacgc tacgccaata ttggtggcta tccctggccc ctctcccacg
    12361 gaaagcacat ccggattttt accatccgcg acaccgtgaa acgccacacc cacacgttca
    12421 ctcaccaccg tgctacattc ccgtaaatga ataacaaaag gcaccggtgc gctatcttcc
    12481 cctaccgcat gaaaccggtt actgctgatt tgccccatat tgaccgtcat ttgtttatca
    12541 ccggcttcaa tccggcaagt ttccgcaata atgacgccct gaaattgcat atttccgccg
    12601 ggcagcgtgg tattccattt atttccggcc agagcaaaca ttggcagcaa cgacgctaat
    12661 agaaataatc tcttcctttt catcgttatt tttatcgcac aagggtgggc atccctgccc
    12721 gtaatgacgt ccctgaacct gggtaggtta ttgatactga accttgaagg tcgcatccgc
    12781 attagcagca cccggggttg cggcaccggt tgcaaaataa cgcgcctgga acggaatggt
    12841 gttggttccg tttttcaggg tagtttctga actaaatgtc gcaccgtcca gcgccagcgc
    12901 agcacccgtt ctgtccagga tctgcacacc aacgtttgtt gcgctacccg cagctgaact
    12961 ctgcagagcc agaacgttgg tatgacccgc atcaatcgcc gtacctaaaa aggcaacagc
    13021 ggctttagat gcaacattgg tatcgcaatc attcagctga atgttaaaac cgacagcaga
    13081 actggtcgct ccttcctgtg ccagcgatgc ggtacgaacc tgtcctaact gaacggtttg
    13141 atcaacagag cctgcatcaa ctgcgcaagc ggcgttaaca acttcccctt taaagtgaac
    13201 ggtcccacca tttaccgtcg tggcagcggc cagagccgct gtagaactga gggacagagc
    13261 cgacagaaca acgattgcca gagttttaat tttcatgctg ctttcctttc aaaaaactat
    13321 ttctaaatcg acatgggcag tcgttctgta cactttgttt tgtcaacgag tttgactgcc
    13381 aacactgcac agttttcccc caaaagatga aacatttggg gccattttga ctcatagagg
    13441 aaagcatcgc ggacaaactt tttcagttta tttgttggct taatattcta ttgttatctt
    13501 tatttataga tttttatatt gcatgaggtg gtttttggag agaagaatga ggaagatgcg
    13561 tcgagccaca gaaacgttag ctttacatat agcggaggtg atgtgaaatt aatttacaag
    13621 agaaataatt tacatatcaa acagttagat gctttttgtc gttttttaat atttttatgc
    13681 ttgag
//
LOCUS       DAZKIV010000126        13632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZKIV010000126 DAZKIV010000000
VERSION     DAZKIV010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.126"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            23..1789
                     /gene="adeD"
                     /locus_tag="ACHOT6_003583"
     CDS             23..1789
                     /gene="adeD"
                     /locus_tag="ACHOT6_003583"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFP9948742.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(1834..3225)
                     /gene="uhpT"
                     /locus_tag="ACHOT6_003584"
     CDS             complement(1834..3225)
                     /gene="uhpT"
                     /locus_tag="ACHOT6_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFP9948743.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3363..4682)
                     /gene="uhpC"
                     /locus_tag="ACHOT6_003585"
     CDS             complement(3363..4682)
                     /gene="uhpC"
                     /locus_tag="ACHOT6_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFP9948744.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATRFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4692..6194)
                     /gene="uhpB"
                     /locus_tag="ACHOT6_003586"
     CDS             complement(4692..6194)
                     /gene="uhpB"
                     /locus_tag="ACHOT6_003586"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFP9948745.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6194..6784)
                     /gene="uhpA"
                     /locus_tag="ACHOT6_003587"
     CDS             complement(6194..6784)
                     /gene="uhpA"
                     /locus_tag="ACHOT6_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFP9948746.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6859..7149)
                     /gene="ilvN"
                     /locus_tag="ACHOT6_003588"
     CDS             complement(6859..7149)
                     /gene="ilvN"
                     /locus_tag="ACHOT6_003588"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFP9948747.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7153..8841)
                     /gene="ilvB"
                     /locus_tag="ACHOT6_003589"
     CDS             complement(7153..8841)
                     /gene="ilvB"
                     /locus_tag="ACHOT6_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFP9948748.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8947..9045)
                     /gene="ivbL"
                     /locus_tag="ACHOT6_003590"
     CDS             complement(8947..9045)
                     /gene="ivbL"
                     /locus_tag="ACHOT6_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFP9948749.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9610..9699
                     /gene="tisB"
                     /locus_tag="ACHOT6_003591"
     CDS             9610..9699
                     /gene="tisB"
                     /locus_tag="ACHOT6_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFP9948750.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9962..10036)
                     /gene="ysdE"
                     /locus_tag="ACHOT6_003592"
     CDS             complement(9962..10036)
                     /gene="ysdE"
                     /locus_tag="ACHOT6_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFP9948751.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10118..11302
                     /gene="emrD"
                     /locus_tag="ACHOT6_003593"
     CDS             10118..11302
                     /gene="emrD"
                     /locus_tag="ACHOT6_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFP9948752.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11310..11807)
                     /gene="yidF"
                     /locus_tag="ACHOT6_003594"
     CDS             complement(11310..11807)
                     /gene="yidF"
                     /locus_tag="ACHOT6_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFP9948753.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PHLEFDDRGDMTAASVTAELWGKFLIALFECWVRADISRISIELFDSTLQKWCGSENP
                     QSRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11804..12166)
                     /gene="yidG"
                     /locus_tag="ACHOT6_003595"
     CDS             complement(11804..12166)
                     /gene="yidG"
                     /locus_tag="ACHOT6_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFP9948754.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12156..12503)
                     /gene="yidH"
                     /locus_tag="ACHOT6_003596"
     CDS             complement(12156..12503)
                     /gene="yidH"
                     /locus_tag="ACHOT6_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFP9948755.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            complement(12674..>13632)
                     /locus_tag="ACHOT6_003597"
     CDS             complement(12674..>13632)
                     /locus_tag="ACHOT6_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFP9948756.1"
                     /translation="ERAYKNFFQNRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFLP
                     KLGWMRYRNSRQVTGVVKNVTVSQSSGKWYISIQTESEVSTPVHPSASMVGLDAGVAK
                     LATLSDGTVFEPVNSLQKNQKKLARLQRQLSRKVKFSNNWQKQKCKIQRLHSRIANIR
                     RDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVREKSGLNRSILGQGW
                     YEMRRQLEYKQLWSGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         3178 a         3815 c         3576 g         3063 t
ORIGIN      
        1 ataaaattaa aatgagaaaa atatgaataa ttctattaac cataaatttc atcacattag
       61 ccgggctgaa taccaggaat tgttagccgt ttcccgtggc gacgctgttg ccgattatat
      121 tattgataat gtctctattc tcgacctgat caatggcgga gaaatttccg gcccaattgt
      181 gattaaagga cgttacattg ccggtgttgg cgcagaatac gctgatgctc cggctttgca
      241 gcggattgat gctcgcggcg caacggcggt gccagggttt attgatgctc acctgcatat
      301 tgaatccagc atgatgacgc cggtcacttt tgaaaccgct accctgccgc gcggcctgac
      361 gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg ggagaagccg gattcgcctg
      421 gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac ttacaggtca gctcttgcgt
      481 acccgccctg gaaggctgcg atgttaacgg tgccagtttt acccttgaac agatgctcgc
      541 ctggcgggac catccgcagg ttaccggcct tgcagaaatg atggactacc ctggcgtaat
      601 tagcgggcag aatgcgctgc tcgataaact ggatgcattt cgccacctga cgctggacgg
      661 tcactgcccg ggtttgggtg gtaaagaact taacgcctat attactgcgg gtattgaaaa
      721 ctgccacgaa agttatcagc tggaagaagg acgccggaaa ttacaactcg gcatgtcgtt
      781 gatgatccgc gaagggtccg ctgcccgcaa tctcaacgcg ctggcaccgt tgatcaacga
      841 atttaacagc ccgcaatgca tgctctgtac cgatgaccgt aacccgtggg agatcgccca
      901 tgaaggacac atcgatgcct taattcgccg cctgatcgaa caacacaatg tgccgctgca
      961 tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccac tttggtctga atcacctcgg
     1021 cttactggca cccggcaagc aggccgatat cgtcctgttg agcgatgcgc gtaaggtcac
     1081 ggtgcagcag gtactggtga aaggcgagcc gattgatgcg caaaccttac aggcggaaga
     1141 gtcggcgaga ctggcacaat ccgctccgcc atatggcaac accattgccc gccagccagt
     1201 ttccgccagc gactttgccc tgcaatttac gcccggaaaa cgctatcggg tcattgacgt
     1261 catccataac gaattgatta cgcactccca ctccagcgtc tacagcgaaa atggttttga
     1321 tcgcgatgat gtgagcttta ttgccgtact tgagcgttac gggcaacggc tggctccggc
     1381 ttgtggtttg cttggcggct ttggactgaa tgaaggtgcg ctggctgcga cggtcagcca
     1441 tgacagccat aatattgtgg tgatcggtcg cagtgccgaa gagatggcgc tggcggtcaa
     1501 tcaggtgatt caggatggcg gcgggctgtg cgtggtacgt aacggccagg tccaaagtca
     1561 tctgccgttg cccattgccg gcctgatgag caccgacacg gcgcagttgc tggcggaaca
     1621 aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg ttacccgatg agccgtttat
     1681 tcagatggcg tttctttctc tgccagtgat ccccgcgcta aaactaacca gtcaggggct
     1741 atttgatggc gagaagtttg ccttcactac gctggaagtc acggaataat aaaaaagccc
     1801 ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc actgtcaact gctgaatttt
     1861 tttctcgcgg cggattttgc gttcttccat caccgccact atcgccatca ggcagataca
     1921 accaatcgcg gcgatatcaa gtgcggcgaa ggtgcctgcc cagccggtaa ggccgaatac
     1981 cggcgtccca tcggcaatca ttcccagacc taacttggca aagctgtcac caatcaggta
     2041 agcaaaggtg cctttaatac catcggcagc gccaatcgct tttttaggta caaagccaac
     2101 agcagccaca ccaatcaaca attgcgggcc aaagaccagg aaacccaacg caaagagaga
     2161 agccagatag atatattcgt tgctggcatg ttgatacaca ccgagcgtgg cgataatcag
     2221 cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc gccaggtcag agagccagcc
     2281 ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc gtaaagccct gaatcgccac
     2341 cgctttagag agtttcagtt cctggaacgc gtatacggtt gaccactggt cgataccaat
     2401 acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc cagatcactt tgtttttcag
     2461 cacatactca acaaagatct gccacttggt catatcggta gattctgtct ctttgtcctc
     2521 ttcgctgatc tcctcgccga acagttcttc agctttgccg aggccataag attccgggga
     2581 gtcgctgccg taacgcaggc cgataaaacc gacaatcagc gcgataatcg acgggaagat
     2641 aaacatgccg atgacatggc catcgaacag gtaatttgcc ccgaacagcg ccacacctgc
     2701 tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa ccgaggaatg tcccgcgttt
     2761 acgacgcggc gtccatttgg tgatggtgga gtaactgcac gaaccgccgg tactctggaa
     2821 aaagccgctt aaggcgtaga aggcaatcat caggaacagg ctaaccgagc cgctgcccat
     2881 actggcactg aagcccagca tacaaatggc agacaggatc agcatgaacg gcaggaattg
     2941 tttggtgttt ttgccgtcgg cgtagtagga aaccagcgtt ttacccacgc cataagtgat
     3001 ggagaaaccc aggccgatca tccccagctg cgtcatgctc aacccgtagg tcgagatcat
     3061 gtcgttctgc gcgatgttaa agttcttgcg aatcaggtac atcgtcaggt agccgataaa
     3121 gaccaccagg taggattgca tgaacggttt gaaccacatt ttgcgccgca cttcgagcgg
     3181 aaggtccagg gtcggcttgc gaacctggtt taagaaagcc agcatgggtt actcctgaaa
     3241 tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc aaatcaggcc tgagatagcg
     3301 tccaggcaaa acctggaaaa tttcttagtt ttgccggata tgaagtgaaa aggtgagatg
     3361 catcacgctt cgcgcggtgc ctgggcgttc aaaaagggca gtaacagcag tgcggaaatc
     3421 ccggcggcga tagagataac cacaaaaaat ccgctccagt gccaggtatc gagtactttc
     3481 gccagcggcc aaccagcaag cgacgccccc agataagcaa acaagccgac aaaccccgtc
     3541 gccgcccctg ccgcctcttt gtgggaacac tctgccgccg ccatgccgat taacatctgc
     3601 gggccaaaga caaaaaaacc aatggtgaag aagcgggttg cctgcatcac gtagctggca
     3661 aatggcatca gccacagcga gccgactgaa agcaaaattc cggcggcgaa aatcaaattc
     3721 atcggcccac ggttgccgtt aaacaatttg tccgagcccc aaccggctac cagcgcaccg
     3781 ataaatccgc ccagttcaaa catcgtcact gccgtattcg ccgtgaccag atcgacgccc
     3841 agtgtctcgg acatatacaa attgccccag tcgttgatcg ccgcccggac cacatagacc
     3901 agcacatagc aaaacgaaag cagccagata tacggattca gcaacacata tttggtgagg
     3961 atctctttac gcgtcaaccc tgccccttct tgttgttgag caatttccag cgcgtcgtgt
     4021 cgccattcac cgaccgccgg taaacctaac gcctgcgggc gatcgcgtag ccgccagcag
     4081 agaaaaatcc ccacgactat cgccatacaa ccagcaatca tcatcccggc acgccagccg
     4141 taatgcagcg cagccgctgc catcacaatg ggaatgagtg cgccgccgac gttatgcgcc
     4201 gtgttccata atgcccacca accgccgcgc tcggtacgtg aataccaggc cgttaacaga
     4261 cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt tcagcaccca gagcacggca
     4321 aacgcccata gcgacgtcga gaagccaaac agaatgttga tgatgcccgt ggcgataagc
     4381 cctatcccca taaaataacg ggcatttgag cgatcgctga caatgccgga gacaaacttc
     4441 gacacgccgt aggtaatgta aaacagggtc gctaacaggc cgatatcgct acggctgagc
     4501 acgccgttag caaggatttc tggtacggcg gcgttaaaac ttttacgcgt gaagtaaaac
     4561 agcgcgtaac ccagccagat ggtcagcaga atatgccgac gccagtagcg atagcgggca
     4621 tcaatttcat gtttgtcagt cattaatggc gcatcggcag gcgctttcag aaacggcaac
     4681 atcatcgaac cttagacata gcgttgaggt agagaaacgc tgacacgcgt gccgtgcaga
     4741 caggaaatgt gtaatgtgcc gcccagcgcc gttacgcgct cgcgcattcc ggtgaggcca
     4801 aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat cgtcttcaat caccagcatc
     4861 aaccgttcat cctgctgcca gccttgcagc gtgaccgcgc tggcatcagc atgtttcaca
     4921 atgttgttca gcccttcctg gcagacacga aacagcgtca cgcgctggtt ttcacttaat
     4981 gctgattcat cgattcgcca ttcgagatgg ctgacaatgc cgcgcccttc cagctccatt
     5041 tcccgcatca gtgagcggat ggcctgctcc agggagagat catccaactg gcgcggacgt
     5101 aaccgtccta ataaacggcg caccgcgtcg tacacgccca gcgacagttg ttcgatgagc
     5161 tgcccgctct gcttcacgct ggcgttatct gccgccagcc gctgaacaat gcccgcctga
     5221 gtacgaatgg ctgtgatggt ctgaccgata tcatcatgca gctcacgcgc cacatcacgg
     5281 cgcacgctct cttcggtttc cagcaaccgt tcagccagat gctgattgcg cgccagttcc
     5341 ttttgcagcg actggttaag ttcacgcaac cgctggatgc cagcgccaag caacaaccct
     5401 gtcagacttt gcaccagcag cgagagcaat aaatccaccg gatgatcgcg ccaggtttga
     5461 ctggcgatca gcgcgatggc gttcatcaac gtcgcaatca gcgccccttg ccaaccatag
     5521 tgccaggcca gcgcgataat cggcagcgcc agacaaaatg gcgtaaagcg cgacagttcg
     5581 tccggcaatc ccaactggag ccagagactg ataacaaaca gcagcaagta ccagaccaga
     5641 tgtcgcccgc gccagttgat cggctgagaa accagtgacg ggccgagcgg cagccaggtg
     5701 ttattggcga gatagtgcca gaacaccaga catatcgggg ccagcgtcag gccgccagtt
     5761 aaagtcagca acagcgcatt ccacgactct ttgccgtgcc aaagccaggg cagcgactgc
     5821 aacaacgccg ccgccgttaa cgccgcaccc tgtaacagca aggtgcgcca gtcacgctga
     5881 tggcgatagc gcgagatcag cgccacgggc agtaacgtca gtaaactacc gatcatcaat
     5941 aacggaaaat gggttaaacc gaccgcctgc gttagccagt aaatcagcag ccactccgcg
     6001 cccagcagca ccggccagta tccgcgcggg cattgcagca ttagccccag acgcagacca
     6061 aacggaaata acagcaccgc catatcaggg cgctcaacca ggtgcaggct gatactccac
     6121 aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa taacggtaat taagcgggag
     6181 aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc cagctctacg tcgttactga
     6241 cacccagttt ttccatcagg ttggcgcgat ggacgtgcac cgttttcggc gacaagccca
     6301 gttcggcagc aatctctttc accgccattc cttgcgccag tttttccgcc acctgccgtt
     6361 cgcgtttggt tagcgggtcc tgacgaccgg atgccagttt aatggcaata tccggcgtca
     6421 gataacagcc gcccgtggca accgtatgca ccgccgcaat cagttcgtca gggctacagc
     6481 gtttggagag aaagccgcgt gcccccgcgt taagcgcctg ctcaaccagc gccggactgt
     6541 catgaacaga gagcattatt gtcgccatac ctttcggcag ctggcttagc agttccagac
     6601 cggagatatc gggcatggag atatcgcaaa tacacacctg cacaccgcgc cccggcagcc
     6661 ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac ctgcaaatca ggttccagcc
     6721 ccagcagctg cgcaaagccg gagcggacga tgaggtgatc gtctataagg gcaacggtga
     6781 tcatggtctt gtcctggcgg gtaaaaaaaa acgcgcttac cttaacgata agcgcgatgt
     6841 tgttcaagcc ttgagcggtt actgaaaaaa caccgcaatc ttgttaaaca tagtcggatc
     6901 ggactgatta cgctgcactt tcaccacatc ttccagctta tcgatttggc ttatcatctg
     6961 ctccagacgc tggtcgtcat tgaccagtag ccagatatgg cttttgtcgc tgtcctgaat
     7021 cggcagacaa agaatgcctt caacgttaaa agcgcggcgg gcaaaaaggc cacaaacgtg
     7081 ggtcattacg cccggatggt tgcgaacggt gagctccaga atcacgttgt catgagttgt
     7141 gttttgcatg gcttattccc ccaccatttc agtattcgcc gcacctggcg gcaccatcgg
     7201 gtaaactttt tcttcggcat caatgcgcac atggatcagc gccgggccag ggcgattgat
     7261 gatttcctgc aatgcagcct gcggatcggc ttcgttattc aaatcacagg tttcgaggcc
     7321 gaatccggcg gcaatctgca taaagttgat ttttcccgga taggtggcgg caaaaacgcc
     7381 ttgctcgtag aacagactct gttgctgatg caccagcccc agcgcttcgt tgttcatcag
     7441 aatgattttg acatccagct gattttcact ggcggtcgcc atctcctgaa tattcatcat
     7501 caggctgccg tcgccggaga aacacaacac tttgcgatcc gggttcgcca gcgccgcgcc
     7561 aatcgccgca ggcaggccaa aacccatcgt gcccagccca ccggaggtca gccactggcg
     7621 tgggcgattg agcggataag cttgcgcggt ccacatctga tgctgaccca catcggtggt
     7681 gataatcgca ttgtcatcga cacaggcggc aacggcgttg atcaggccgt aatggcttaa
     7741 tggatcgcac gctttcggga ttggacacgg gaattcgcgc tgcaaatccg ctaccaactg
     7801 gtgccactct gcacgcggtt gcgcttccac ctgcgggatc aattgcgcca gcacgtcatc
     7861 aacatccgcc tgaatcgcca catgcggctg cttgatttta cccagctctg cgcgatcgat
     7921 atcgacatga ataattttgg cattcggaca gaactgctcg gttttgccaa tcgcccggtc
     7981 atcaaaacgc gcaccgagca ctatcaacaa atccgcctcc tgcaaaatat agttggtgct
     8041 gcgcacgccg tgcatcccca gcatacccag cgacaacgga tgcgcttttg gcaacatgcc
     8101 cagcgccatt aaagtcatgg tggtaggcag ttgcgctttc tccgccagtt cacgcacccg
     8161 tgcgggcgca ttgatcacac cgccgcccag ataaagcacc gggcgtttgg cagcgttaat
     8221 catcgccgct gcgtcacgaa tgctttcttc gctaaaggcg ggggcggcgg ctttttctgc
     8281 catagcgggc tgtgtttcaa tctcaaaaac tgccgtttgc acatccttag gaatgtctat
     8341 ccacaccggg cctgggcggc ctgattgcgc aatgcggaag gcatcgctca tgacctgcgg
     8401 gagttcttcg atatgtctga ccagatagtt gtgtttggtg atggggatag agatgccgta
     8461 ggtgtccact tcctggaagg cgtcggtgcc gatcatcgag gcgggaacct gaccggtgat
     8521 gcaaatcagc gggatggagt ccagccgcgc atcggcaatg gcggtcacca ggttagtcgc
     8581 acccggtccg ctacaggcca tacagaccgc cggtttaccg tcggtgcgcg ccatcccctg
     8641 agcgataaat cccgcgccct gttcatgacg ggccagaata tggcggattt gcgtgctttg
     8701 gcttaaggca tcgtaaacag gcaggataga accgcccgga atgcccgtca caatcttaat
     8761 gccctgctgt tccaggaaat gaacgataaa ttctgcgccg gtaaagcgct tacgcgtcga
     8821 tgttgtgccc gaacttgcca tgctccagtc cttttcttct gggccgactt tccgggaggt
     8881 gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt tttggaatcg tgtgttgttc
     8941 cagtccctac ggcgcattgc cgacgaccac caccacacgc acgacgacca ctgcggcgga
     9001 tagcgcagtt ggtagtagtt ttgcgttgaa catggaagta gtcattgggg accttgttgg
     9061 ttttgtgttt aacaatattt atacaagcac agctttacag gggagacaat ggaaaatttt
     9121 tcagcaaggg aaaattgagg ggctgatcac gttttgtact gaattgcagg taacaaaaaa
     9181 ccccgccgga gcgaggtttc atcagccgcc tgcggctggt aaccgcaaag cacactgtat
     9241 tatgtcaaca ctgaaagtat acgtgttccg cgcagaacgc gcaatttcgg cacgaatttt
     9301 gacgtattta gtgcatagtt gagtatcgat cacagtttgc attttgtcca aatattactg
     9361 tttatttata cagtaaactt ctataatatc actgtacgca atgtgttatg cgggggccgc
     9421 atcgttaccc ggcgcactaa gtcctggctg aaacggatgg tgccgtcagc gccttaaccc
     9481 cgcgtgagca cactgtgtta tgtcaacaag cacaacgttt ctccttgaga taccgcgtgc
     9541 acaacagctg gcaacaggca gcggaaaggt acgtcagctg gcagtgctcc tgaaccacag
     9601 gagacgcgta tgagcctagt ggatatcgcc attcttatcc tcaaactcat tgttgcagca
     9661 ctgcaactgc ttgatgctgt tctgaaatac ctgaagtaat tcagattcaa gtcgcaccaa
     9721 aggggagcgg gtaaccgctc cccttttata tttagcgtgc gggttggagt tgggggcttc
     9781 ttctaccagc agaatgtttg cacctgcgct gcctgtgccc ctcaccctag ccctctcccc
     9841 aaaggggcga ggggaccgtc ccgtgcaatg tttagctcat tttctgaaag ggaacggtgg
     9901 tttccccctc gcccctttgg ggagagggtt acagttcgca cgtaatccct cccggcaaca
     9961 tttattgccg cttttgttta catattctgc cgctaaacaa ttccccattc ctggcgtata
    10021 tctggctaac attcatcaat gtgatagatt cctctcccgc atttatggga atgcgtagtg
    10081 acttattcta attattttta taaaagcatc cgtgataatg aaaaggcaaa gaaacgtcaa
    10141 tttgttattg atgttggtat tactcgtggc cgtcggtcag atggcgcaaa ccatttatat
    10201 tccagctatt gccgatatgg cgcgcgatct caacgtccgt gaaggggcgg tgcagagcgt
    10261 aatgggcgct tatctgctga cttacggtgt ctcacagctg ttttatggcc cgatttccga
    10321 ccgtgtgggt cgccgaccgg tgatcctcgt cggaatgtcc atttttatgc tggcaacgct
    10381 ggtcgcggtc acgacctcca gtttgacggt gttgattgcc gccagcgcga tgcaggggat
    10441 gggcaccggc gttggcggcg taatggcgcg tactttgccg cgagatttat atgaacggac
    10501 acagttgcgc catgctaaca gcctgttaaa catggggatt ctcgtcagtc cgttgctcgc
    10561 accgctaatc ggcggtctgc tggatacgat gtggaactgg cgcgcctgtt atctcttttt
    10621 gttggttctt tgtgctggtg tgaccttcag tatggcccgc tggatgccgg aaacgcgtcc
    10681 ggtcgatgca ccgcgcacgc gcctgcttac cagttataaa acgcttttcg gtaacagcgg
    10741 ttttaactgt tatttgctga tgctgattgg cggtctggcc gggattgccg cctttgaagc
    10801 ctgctccggc gtgctgatgg gcgcggtgtt agggctgagc agtatgacgg tcagtatttt
    10861 gtttattctg ccgattccgg cagcgttttt tggcgcatgg tttgccggac gtcccaataa
    10921 acgcttctcc acgttaatgt ggcagtcggt tatctgctgc ctgctggctg gcttgctgat
    10981 gtggatcccc gactggtttg gcgtgatgaa tgtctggacg ctgctcgttc ccgccgcgct
    11041 gttctttttc ggtgccggga tgctgtttcc gctggcgacc agcggcgcga tggagccgtt
    11101 ccccttcctg gcgggcacgg ctggcgcgct ggtcggcggt ctgcaaaaca ttggttccgg
    11161 cgtgctggcg tcgctctctg cgatgttgcc gcaaaccggt cagggcagcc tggggttgtt
    11221 gatgacctta atgggattgt tgatcgtgct gtgctggctg ccgctggcga cgcggatgtc
    11281 gcatcagggg cagcccgttt aagcgcacgt caccgcagca tcgtcatcag ctccatggga
    11341 gaacgatgct gctttatcag atcacgcatc acccgcatat gcggtgcgga gtaagaataa
    11401 aacgcctgat agcccgcaca aagcacgctg tctggcgtct cctgccgagc gtgcgggcac
    11461 aggcgatgcc agtcgcacgc ctggcagtca cggcgcgatt gcgggttttc gctgccacac
    11521 catttttgta gggtggagtc aaaaagctca atcgagatac gactgatatc tgcccgcacc
    11581 caacactcga acagggcaat taaaaatttc ccccacagct ctgctgtcac cgaagcggcc
    11641 gtcatatcgc cccggtcatc aaattcaaga tggggaatga gctgtacgga gcggatgccc
    11701 cgttgtttga aattgtgata aaaatcggca ggttgcagag catccttgta ctctacaacc
    11761 agtttatgcc tgtcctcttc agcatcaatg acctgacttc ctgtcatgcg actctctcaa
    11821 taaatacgat aagttgatgt atatgcgtta cagcaaacag tattgcgaga gataacaccg
    11881 cgagggagat caaaaatttg tcacggacta cgtgaaattg ggaaaaatcg ctattcgtga
    11941 catccattaa attgcgatta cgggtgtagt gccagaggat cagcgccacg atggcgagga
    12001 tgccaatcga aatccagaat aacatgcccg cctggtgcca gttgtgtttg atagccaacg
    12061 ccatcaacgc gccgtagccc agcatggtac gaaaccacgc cagtgatgtg cgctccggct
    12121 gcagtcctgg gtcggcaatg cgtctggctt tgcggctatc cggcatacaa caccagtccc
    12181 ataacgatca ccgccacgac catcaatatt aagctaatga ttaataagct gttggtgtag
    12241 ggcaggtcct ctttcagacg catcgctttt tcattgcgta accagcgcag gtagccatac
    12301 attgctaacc cgccggaaaa cagacacaac aacaacgcca gcagttcacg aatgacgggc
    12361 gtggcgaaat ctggcgcaag ttgatcaagc ccaacccccg ccgccagaaa tcctagtgcg
    12421 gtacggatcc acgccagaaa agtacgctca ttagccagcg agaagcggta atcgggcgct
    12481 tctccgaggc gggaaatttt cattgttgct cctttgcaat ttcactaacg ctctatcttt
    12541 agcggttttt tttaagaatt tcgtccgccc gtttcctgat atgcttttgc catccgctac
    12601 aaggaggtca gatgggtatc atagcgcaaa ttgacatcct ccacgccctg aaggacgtgg
    12661 atccctgcta cgttcaggct gtcgcctgaa tcatttcggt gggttcctgc ttcaacgggc
    12721 ggcctgactg caccatctct ccacaggcaa gaacggcgtg ccccgccgct aaaatgttac
    12781 gagcgccatt tacatttaca tcggcgttcg ctgtatatcc acatacctgg catctgaatt
    12841 tactttgtga caggcgattt tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg
    12901 tgtacgctgg cggaacagca agcacctgac cgccactcca gagctgcttg tactcaagct
    12961 ggcggcgcat ttcataccag ccctgaccca gtatcgaacg gtttaaacct gatttttccc
    13021 ggacattgcg ccccggctgg ctgaccgtac ccgctgctga ctttgacatg ttgctgacct
    13081 tcaaatcctc aatgactatc attgcgtggt ttttgctgac ggtcgttgtg actttgtgaa
    13141 ggtagtccct gcggatattt gcgatacggg aatgcagtcg ctgtattttg catttctgct
    13201 tctgccagtt gttgctgaat ttgaccttgc ggcttaactg ccgctgaagt ctcgccagct
    13261 tcttctggtt tttctgcaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg
    13321 cgagtttagc cacgccagca tccagcccga ccattgatgc tgaagggtga accggagtgg
    13381 atacttcact ttctgtctga atactgatgt accacttacc gctggactgg ctgaccgtga
    13441 catttttcac aactcccgtg acctgacggc tattccggta gcgcatccag ccaagtttcg
    13501 gcagaaaaat acggctgttt tcctgatcga gcttaacgcc ctgcgggtag cggaatgcat
    13561 cattctgtcc ccgctttttg aatcggggaa aagcagcccg attctggaag aagtttttgt
    13621 aagcccgctc aa
//
LOCUS       DAZKIV010000127        13538 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZKIV010000127 DAZKIV010000000
VERSION     DAZKIV010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.127"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..442
                     /locus_tag="ACHOT6_003598"
     CDS             <1..442
                     /locus_tag="ACHOT6_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9948757.1"
                     /translation="IDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQLMLFNLF
                     SPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPINKAYTPYNIHTYKTEEVVNQRN
                     IKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            766..2154
                     /gene="narU"
                     /locus_tag="ACHOT6_003599"
     CDS             766..2154
                     /gene="narU"
                     /locus_tag="ACHOT6_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFP9948758.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            2236..5976
                     /gene="narZ"
                     /locus_tag="ACHOT6_003600"
     CDS             2236..5976
                     /gene="narZ"
                     /locus_tag="ACHOT6_003600"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFP9948759.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGTAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5973..7517
                     /gene="narH"
                     /locus_tag="ACHOT6_003601"
     CDS             5973..7517
                     /gene="narH"
                     /locus_tag="ACHOT6_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFP9948760.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7517..8212
                     /gene="narW"
                     /locus_tag="ACHOT6_003602"
     CDS             7517..8212
                     /gene="narW"
                     /locus_tag="ACHOT6_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFP9948761.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDAMALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGEK"
     gene            8209..8889
                     /gene="narI"
                     /locus_tag="ACHOT6_003603"
     CDS             8209..8889
                     /gene="narI"
                     /locus_tag="ACHOT6_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFP9948762.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8969..9862
                     /gene="yddE"
                     /locus_tag="ACHOT6_003604"
     CDS             8969..9862
                     /gene="yddE"
                     /locus_tag="ACHOT6_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFP9948763.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(9947..10792)
                     /gene="nhoA"
                     /locus_tag="ACHOT6_003605"
     CDS             complement(9947..10792)
                     /gene="nhoA"
                     /locus_tag="ACHOT6_003605"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFP9948764.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGDYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10967..11536
                     /gene="yddH"
                     /locus_tag="ACHOT6_003606"
     CDS             10967..11536
                     /gene="yddH"
                     /locus_tag="ACHOT6_003606"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFP9948765.1"
                     /translation="MSRFIPVELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLRLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11540..11683)
                     /gene="pptA"
                     /locus_tag="ACHOT6_003607"
                     /pseudo
     CDS             complement(11540..11683)
                     /gene="pptA"
                     /locus_tag="ACHOT6_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
     gene            complement(11831..13153)
                     /gene="ltrA"
                     /locus_tag="ACHOT6_003608"
     CDS             complement(11831..13153)
                     /gene="ltrA"
                     /locus_tag="ACHOT6_003608"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HFP9948766.1"
                     /translation="MNVERRGSVGQSASQPNCKQEEAAGEQTKPFQVSKLHVVEAYRR
                     VKANAGAAGVDNQTLKDFERDLKGNLYKIWNRLSSGSWMPPPVRAVEIPKKDGSKRLL
                     GIPTVSDRIAQMTVLVTFEPLVERYFLNDSYGYRHGKSALDAIAVTRKRCWQYDWYLE
                     FDIKGLFDNIPHDLLLRAVDKHCADKWVRLSIRRWLTAPVQMPDGTLKERNKGTPQGG
                     VISPVLANLFLHYVFDKWLSLHYPEIPWCRYADDGLIHCGSKQQAEELLNKLAKPFQE
                     CGLELHPEKTKIVYCKDSERQANHETVQFNFLGYTFRARRARNQRRGNLFTSFSLAVS
                     NSAQKDMIGKLRKLRLRRRVEMSLEDIAKRLNPMISGWLNYYAKYYKSAMKKVCRYIN
                     LTLIAWARKKYKTLRYKKTKACQLMERLSKEKPELFAHWKAGPGSAFA"
BASE COUNT         3212 a         3553 c         3386 g         3387 t
ORIGIN      
        1 cattgatact aataatacat ccgatattgg aactttattc cccataataa aacagagcaa
       61 aaacttaaat tttttaaatg tttctgggaa gaacaattgc cctactatgc agctcatgtt
      121 atttaattta ttttccccag cacttaagct taatactggc ctggcaattc tttcgcctgg
      181 tgcatttgaa gttcactctg acggaataga tgtggataac gaattgtttc actatcctat
      241 taataaagca tataccccat ataatataca cacttacaag acagaggaag ttgtaaacca
      301 gaggaatata aaagttaaaa acatgacctt agatgaaata aacaatactt actgtaataa
      361 cgattattac aatcaggcaa taagagagga accgatagac cttctggaca gatcgttttc
      421 ctccagttca tggccttttt agtgaataaa tcatcactaa ttctataagt gtctggtctt
      481 gttgataata tagttaatat gtaacccttt agattcacga tctgaagggt tgaaacatga
      541 catacgttca aagattaacc cccgtttgaa ggttagcttg ttagttacat ttagtaacac
      601 atatctggct acttaatccc cattctgccc ccgcggttac gctttcttat ctattctttc
      661 aatcagcact tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc
      721 tgttttgtct tttttcatta ccaatgtgtg catgtgagga acaatatggc actgcaaaat
      781 gagaaaaata gtcgttatct tttacgcgac tggaaaccag aaaatccggc cttctgggaa
      841 aataaaggaa aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt
      901 gccttctgtg tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt
      961 aattttacta ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta
     1021 ttgcgtgttc cctactcctt tatggtgcct atattcggtg gacgccgatg gacggttttt
     1081 agtactgcaa tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat
     1141 actccttttg ggatatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt
     1201 gcttcgagca tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt
     1261 gggattaatg gcggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg
     1321 gtcatttttg tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt
     1381 tcggtgatgt cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg
     1441 atcgccgcct ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgccgaccag
     1501 ctccctgtct tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc
     1561 ggttcgttta tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat
     1621 gtgaatattc tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt
     1681 ggtggtgcta tttccgataa gttcggcggc gtgcgggtga cgttgatcaa ctttattttt
     1741 atggcgattt tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc
     1801 atcgcctttt acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct
     1861 actttccaga tgatcgccgt catctttcgc cagataacca tttatcgggt aaagatgaaa
     1921 ggcggtagtg atgagcaagc tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc
     1981 tttatctcag ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg
     2041 ctcaatatga ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt
     2101 tgtgtgctgc tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc
     2161 gatgcctgat gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg
     2221 agcaggagtc atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc
     2281 gaaacctttg ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc
     2341 tatcgccagc gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt taactgtaca
     2401 ggctcctgta gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag
     2461 accgactacc cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt
     2521 ggcgcaagtt actcctggta tctttacagc gctaaccgcc tgaaataccc gctcattcgt
     2581 aaacgactga ttgaactgtg gcgcgaagcc ctcaaacaac acagcgatcc ggtactggcg
     2641 tgggcatcga ttatgaacga tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc
     2701 ggcgggttta tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc
     2761 tggaccatca aaacctacgg cccggatcgc gttgccggtt tctcgccgat cccggcgatg
     2821 tcgatggttt cttacgccgc cggaacgcgt tatctgtcac tgcttggcgg cacctgttta
     2881 agtttctacg actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa
     2941 accgacgtac cggaatctgc cgactggtat aactccagct acatcatcgc ctgggggtct
     3001 aacgtaccgc agacacgtac gccggacgcc cacttcttta ccgaagtacg ctacaaaggc
     3061 actaaaacca tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg
     3121 ctggcaccga aacaaggcac tgacagcgcc ctggcgatgg caatgggcca tgtgatttta
     3181 aaagagtttc atctcgataa tcccagcgac tactttatca actactgccg ccgctacagc
     3241 gacatgccga tgctggtaat gctggagcct cgcgacgatg gtagctacgt tcccgggcgc
     3301 atgatccgcg catctgacct ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa
     3361 accgtagcag ttaataccgc aggtgaattg gtagtgccga acggttcgat tggtttccgc
     3421 tggggagaaa aaggcaaatg gaatctggaa tccattgccg ccggtacgga aaccgaattg
     3481 tcgttaaccc tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc
     3541 ggcattgaaa atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg
     3601 cccgttaaaa acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat
     3661 ttggtactgg cgaattacgg cctcgatcgc gggctggaag atgaaaacag tgcgaaagat
     3721 tacgctgaaa tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtgccgcgc
     3781 cagtatattg aaaccatcgc tcgtgaattt gccgatactg cccataaaac gcatgggcgc
     3841 tcaatgatta tcctcggcgc tggcgtcaac cactggtatc acatggatat gaactaccgt
     3901 gggatgatca atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca
     3961 cactatgtcg gccaggaaaa actgcgccca caaaccggct ggttgccact ggcctttgcg
     4021 ctcgactgga accgaccacc gcgccaaatg aacagcacct cgtttttcta caatcattcc
     4081 agccaatggc gctatgaaaa agtctctgct caggagttac tttcaccgct cgccgatgcc
     4141 agtaagtaca gcggtcatct gattgatttc aacgttcgcg ccgaacgtat gggctggcta
     4201 ccttctgcgc cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc
     4261 ggattatccc ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc
     4321 tgcgaacaac cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac
     4381 ctgcttggct cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa
     4441 agcgggattc agggcgagga actcggtgcc agcgacggga tcaaaccgga agaagtcgag
     4501 tggcaaactg cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg
     4561 tccagtacct gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac
     4621 gatatgaaca cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg
     4681 gcgtgggaat cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa
     4741 gtgtgcgtgg gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac
     4801 tctccggcag agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat
     4861 ctgattccag gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg
     4921 tatgaacgct ttacctcgct cgggccattg atggacaaac ttggcaatgg cggtaaaggg
     4981 atttcgtgga atacccagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt
     5041 gatggcacgg cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt
     5101 ctggcactgg caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc
     5161 gagatcaccg ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc
     5221 tttcgcgata ttcaggcgca gccgcgtaaa attatctcca gccccacatg gtccggtctg
     5281 gaaagcgatc acgtatccta taacgcggga tacaccaacg ttcatgagtt aattccgtgg
     5341 cgcacgctgt cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt
     5401 gaaagcctgg tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag
     5461 ataccgccaa acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg
     5521 ggcattcact caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg
     5581 attgtctgga tcagcgaaac cgatgcccga gaactgacca ttgtcgataa cgactgggtg
     5641 gaagtattta acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg
     5701 ccgggcatga ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa
     5761 gtaactggca tgcgcggcgg cattcataac tcggttaccc gcgtttgccc gaaaccaacg
     5821 catatgattg gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcgga
     5881 tcgaaccgcg atgagttcat catgatccgc aagatgaaga acgttaactg gctggatgat
     5941 gaaggtcgcg atcaggtaca ggaggcgaaa aaatgaaaat ccgttcacaa gtcggcatgg
     6001 tgcttaacct cgacaaatgt atcggctgcc atacctgttc ggtgacctgt aaaaacgtct
     6061 ggaccgggcg cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca
     6121 ttggttatcc gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg
     6181 tgaatggcaa gatccgtccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg
     6241 ccaacccggt ggtgccgcag attgatgatt actacgaacc tttcaccttc gactacgaac
     6301 atttgcatag cgcaccggaa ggcaaacata tccctactgc tcgcccgcgt tcactgattg
     6361 acggcaaacg gatggacaaa gtgatctggg gcccaaactg ggaagaactg ctgggcggcg
     6421 agtttgaaaa acgtgcccgc gaccgcaact tcgaggccat gcaaaaggag atgtacgggc
     6481 agtttgaaaa caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca
     6541 gttgcgtggc gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc
     6601 tgattgatca ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa
     6661 aaatctactt caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa
     6721 ttgagtcagg ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg
     6781 gcgtgctgct ttacgacgcc gaccgcattg aggaagcggc gagcaccgag cgcgaagttg
     6841 acctctatga acgtcagtgc gaagtgttcc tcgatccaca cgatccctca gtgatcgagg
     6901 aagccctgaa acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct
     6961 acaaaatggc gatggactgg aaactggcgc taccgctgca ccctgaatat cgcaccctgc
     7021 caatggtctg gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt
     7081 tgccgaaaag cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc
     7141 tcgccaatat gttgagtgcc ggcgataccg gtccggtact gcgggcgctg aaacggatga
     7201 tggcgatgcg ccactatatg cgttcacaaa ccgtggaagg cgttactgat actcgtgcca
     7261 tcgacgaagt aggcctgagc gtcgcccagg tcgaagaaat gtatcgctat ctcgccattg
     7321 ccaactatga agatcgtttt gttatcccga ccagccatcg ggaaatggcg ggcgatgcct
     7381 tcgcagaacg caacggctgc ggttttacct ttggcgacgg ttgtcacggc tcggacagca
     7441 aattcaacct gttcaacagt agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca
     7501 aagcggaggg cgaataatgc agatcctcaa agtgatcggc ctgttgatgg agtatccgga
     7561 tgagctgttg tgggaatgca aggaggacgc gatggcgttg atccgccgcg acgcgccgat
     7621 gcttacggat ttcacccaca acctgcttaa cgcgccgctg ctggataaac aggccgaatg
     7681 gtgcgaagtg tttgaccgcg ggcgcaccac gtcgctgctg ctgttcgaac atgttcatgc
     7741 cgagtcccgc gatcgcggcc aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg
     7801 cctgcagctg gattgtcggg aactgcccga ttatctaccg ctgtatctgg agtatttaag
     7861 cgtactgcct gacgatcagg cgaaagaagg attgctcaat gttgcgccga tcctcgccct
     7921 gcttggcggt cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg atgctctgct
     7981 gcaactggcg ggaagtatac tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga
     8041 gcgcgatgac acccgccagg cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat
     8101 tgaagataac gccacggcgt gtgacagctc gccgcttaat caatatcagc gacgctttag
     8161 ccaggatgtc gcgccgcagt atgtcgacat cagtgcggga ggtgagaaat gattcagtat
     8221 ctgaacgtct ttttttacga tatctacccg tacatttgtg cgacggtgtt tttcctcggc
     8281 agctggctgc gctacgacta cgggcagtac acctggcgcg cctcctcaag tcaaatgctc
     8341 gataaacgcg ggatggttat atggtcgaat ctgttccata tcggcatttt ggggatcttc
     8401 ttcgggcacc tgttcggcat gttaacgccg cactggatgt acgcgtggtt tttgccagtg
     8461 gcagcgaaac aactcatggc gatggtgctc ggcggtattt gcggtgtgtt gacattgatt
     8521 ggcggcgcag gactgctgtg gcgcaggctg acaaatcagc gcgtgcgtgc aacttccact
     8581 acgccggata ttatcatcat gagcattttg ctgatccagt gtctgctggg gctaagtacc
     8641 ataccgtttt cagcacagta tcctgatggt agcgaaatga tgaagctggt gggttgggcg
     8701 caaagcattg tcactttccg tggtggatcg tcagaaatgc ttaacggcgt agcgttcgtt
     8761 ttccgcctgc atctggtatt gggaatgacc atcttcctgc tcttcccgtt caccagattg
     8821 gtgcatgtgt ggagcgcgcc gtttgagtac tttactcgtc ggtatcagat tgtgcgttcg
     8881 cggcgataat tttcaccctg gccggatagc ccttcgctct ccggccactt gcaggctatg
     8941 atagttctcc agccacagga gaacgttaat gaaaccgcag gtgtaccacg tcgatgcttt
     9001 tacctcacaa ccgtttcgcg gcaattctgc cggtgttgtt ttccccgccg ataatcttag
     9061 cgaagcgcaa atgcagctta tcgcccgcga gttaggccat tcggaaactg cttttctgct
     9121 gcacagcgac gacagcgatg tgcgcatccg ctactttacg ccaacggttg aagtgccgat
     9181 ttgcggtcac gcgacagtag ctgcgcacta tgtacgtgcg aaggtgttag gtttaggaaa
     9241 ttgcacgatc tggcaaacat cgctggcagg aaaacatcgc gtgactatcg aaaagcacaa
     9301 cgatgattat cgtatttcgc tggaacaagg tacgccgggc tttgagccac cgctggaagg
     9361 tgaaacacgt gaggcgatta tcaacgcgct tcatctcact gaagacgata ttttgccggg
     9421 cttgccaatt caggtcgcaa caacaggaca ctcaaaagtg atgatcccgt tgaaaccgga
     9481 agtggatatc gacgcccttt cgcctgatct tgctgcgctg agcgctatca gcaaacagat
     9541 tggctgcaat ggtttcttcc cgttccagat ccgcccaggc aaaaacgaaa ccgatggtcg
     9601 tatgttctcg cctgcgattg gtattgtgga agatccggtg actggcaatg ccaatggccc
     9661 gatgggtgca tggttggtac atcacaacgt attgccccac gatggcaacg tgttatgtgt
     9721 taaaggccat caggggcgcg cattggggcg tgacggcatg attgaagtga cggtgacaat
     9781 tcgtgataac caaccggaaa aagtcaccat ttcaggcaca gcggtgattt tgtttcatgc
     9841 cgaatgggca attgaactct gaaaccgttg cctgatgcgc ttcgcctgtc cgagctaatg
     9901 tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct
     9961 ccgggtgcgt atcaaacgcc gccatcacca gcgctaactc atccacggta aagccatgtt
    10021 tcgcatcatc cacgcccaga ccaaactgtt cttgcatcac agcatataat gacgccacat
    10081 ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg
    10141 tcagcgttag cttgccaccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat
    10201 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
    10261 cgcagagatc aaaacggtac atcgactgcc agtgctgatg atgattaaac tgcaacaccc
    10321 aatcatcacc ctcctgcaac aaccgataat ctccgtgtgg cgtggtctgc acgagatcgg
    10381 aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
    10441 tttcctcttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
    10501 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ctccagctcg cgtaacaccc
    10561 gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct
    10621 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtcg aggttttcaa
    10681 acggaatagt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag
    10741 cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt
    10801 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcactggc tattttgtgc
    10861 gatcgcggac atgcatcacg ttcgcaaatc gccaatcccg ttaatttcgt cgcttattgc
    10921 gttacgaact acgcttttaa aacaaccttc ttagcgaggc gataccgtga gccgattcat
    10981 ccccgtagaa ttacaccatg ccagccgtct gttaaatcat ggcccaactg tcttgatcac
    11041 cagtttcgat gagcaatccc agcggcgtaa cattatggct gcagcctggt caatgccggt
    11101 ggagtttgaa ccgccacgcg tggcgattgt ggtagataaa tcgacatgga ccagggagtt
    11161 gattgaacgt aacggtaaat ttggcatcgt tatcccgggc gttgcggcga ctaactggac
    11221 gtgggcggtg ggaagcgtgt cgggtcgtga agaagataaa tttaattgct acggcattcc
    11281 ggtagtgaaa ggcccggtgc ttcgtttgcc tctggtcgag gaaaaatgtc tggcgtggat
    11341 ggagtgccgc ctgctacctg cgacttctgc gcaacaaaaa tacgacacgc tgtttggcga
    11401 ggtggtatca gcagcggcag acgcacgggt atttgtcgaa ggccgctggc agtttgatga
    11461 tgataagctc aatacgttgc atcatttagg tgctggcacg tttgttacta gcggcaagcg
    11521 tgttacggca ggttaagcat tacatgctgt aaccgggttt ctttatcaaa gcctccattt
    11581 ggggctcgat ttcggcatcc cagatagctt gccaagattc tggttgaatc tgctgtagag
    11641 caatgcttat cgagctatct ttactgttca gatggcgaat aatttaggta atgaggaata
    11701 tctcaatttt taaacataaa atgcgaatta tttagtacaa aaagtgtact tctagtgagt
    11761 aggccaccgg aacctccccg acagcctctc gcagaaccgt acgtgaaact ctcgcttcat
    11821 acggctcctg tcaggcaaac gcgcttcctg gtcctgcttt ccagtgggca aatagctccg
    11881 gcttctcttt cgacagtctt tccattaact gacacgcctt cgtcttttta taccgcaggg
    11941 tcttgtattt ctttctcgcc cacgcaatca gcgtcagatt aatatatctg catactttct
    12001 tcatcgctga cttgtagtat ttcgcgtaat aattcagcca ccccgatatc atcggattca
    12061 gccttttcgc aatatcttca agactcattt ccaccctgcg tctgagccgc agtttcctga
    12121 gttttccgat catgtctttc tgcgcactgt tactcaccgc cagactgaaa ctcgtaaaga
    12181 gatttcccct tcgctgatta cgtgctcttc gcgctctgaa cgtatacccc agaaaattaa
    12241 actgcactgt ttcgtggttc gcctgtcgtt cgctgtcttt gcagtaaact attttcgttt
    12301 tctccggatg taattccagt ccacattcct gaaaaggttt tgccagttta ttcagtaatt
    12361 cctctgcttg ttgtttactg ccacaatgaa ttaatccatc atctgcataa cgacaccagg
    12421 gaatttccgg ataatgtaac gacagccatt tatcaaacac atagtgcaga aataaattcg
    12481 ccagtaccgg actgatgact ccaccctgtg gcgtaccttt atttctttcc tttagtgttc
    12541 catccggcat ctgcaccggc gctgtcagcc acctgcgaat agacagtctt acccatttat
    12601 ccgcacaatg tttatccact gccctgagta ataaatcgtg ggggatatta tcgaataaac
    12661 ctttaatatc aaactcaaga taccagtcgt attgccagca acgttttctg gtgactgcta
    12721 ttgcatccag cgctgatttt ccatgccggt atccgtatga atcattcagg aaataacgct
    12781 ccactaatgg ttcaaacgtt accagcaccg tcatctgcgc tattcgatcg cttactgtcg
    12841 gtattcctaa aagtctttta ctgccatcct tcttcggaat ttccaccgca cgcactggcg
    12901 gcggcatcca gcttcccgac gataaccggt tccagatttt gtacagattt cctttcagat
    12961 cccgctcaaa atctttcagc gtctggttat ccactccggc agctccggca ttcgccttta
    13021 cccgccggta cgcttccacc acatgcaatt tactgacctg aaacggtttt gtctgttcac
    13081 ccgcagcctc ctcctgttta cagttgggct gtgatgcaga ctgcccgacg ctgccccttc
    13141 gctccacgtt cattacaaac gcttcctcac tactacgaac agctctgccc cggtttcgca
    13201 catcgatact ctcatactca taatttcggt tatttgtatt tctctcttga cattgcgacg
    13261 accggttccc gtagttcctg caaagagcct gatacagcgt catgcctgct atacgccgga
    13321 caccattcac gcagcattcg gaattcaccc gtaaatttat cccggcggta tcatcatgcc
    13381 tccggttttg atgcctctta ggactaacga cgtttcaaca caggttcggg tttactcatc
    13441 tcactgtatc tcacacagga tcagatcgcg tctgatcgtt ccccctgcac tcactaccat
    13501 ggctctttac cacagcagcc ggggttgttt gggaccga
//
LOCUS       DAZKIV010000128        13284 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZKIV010000128 DAZKIV010000000
VERSION     DAZKIV010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13284
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.128"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..2702)
                     /locus_tag="ACHOT6_003609"
     CDS             complement(<1..2702)
                     /locus_tag="ACHOT6_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9948767.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGAAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVQPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQIRDDALILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWA
                     SLPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDLLPAPLPPYRVLTGMADRFGR
                     TLAYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKPHTASLSSPDSPRPL
                     SAPSFPDTLPGTEYGADSGIRLSAVWLMHDPEYPENLPAAPLVCYDWTPRGELAAVYD
                     RSGTQMRHFTYDDKYRGRMVGHRYAGRPEMRYRYDDAGRVVEQLNPAGLSYRYQYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVYPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSDFSYDALDRLVQQGGFDGRTQ
                     RYHYDLTGKLTQSEDEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLS
                     EGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPP
                     VEWLTYGSGYLAGMKLGDTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQ
                     LQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLE"
     gene            complement(3131..5545)
                     /gene="ybbP"
                     /locus_tag="ACHOT6_003610"
     CDS             complement(3131..5545)
                     /gene="ybbP"
                     /locus_tag="ACHOT6_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFP9948768.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAITGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINGKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(5542..6228)
                     /gene="ybbA"
                     /locus_tag="ACHOT6_003611"
     CDS             complement(5542..6228)
                     /gene="ybbA"
                     /locus_tag="ACHOT6_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFP9948769.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            6199..6822
                     /gene="tesA"
                     /locus_tag="ACHOT6_003612"
     CDS             6199..6822
                     /gene="tesA"
                     /locus_tag="ACHOT6_003612"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFP9948770.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            6812..7621
                     /gene="ybbO"
                     /locus_tag="ACHOT6_003613"
     CDS             6812..7621
                     /gene="ybbO"
                     /locus_tag="ACHOT6_003613"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFP9948771.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVKRMNNMGFTGVLIDLDSPESVDRAADEVSALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            7682..8536
                     /gene="cnoX"
                     /locus_tag="ACHOT6_003614"
     CDS             7682..8536
                     /gene="cnoX"
                     /locus_tag="ACHOT6_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFP9948772.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(8599..9378)
                     /gene="fetB"
                     /locus_tag="ACHOT6_003615"
     CDS             complement(8599..9378)
                     /gene="fetB"
                     /locus_tag="ACHOT6_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFP9948773.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(9365..10042)
                     /gene="fetA"
                     /locus_tag="ACHOT6_003616"
     CDS             complement(9365..10042)
                     /gene="fetA"
                     /locus_tag="ACHOT6_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFP9948774.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            10188..11105
                     /gene="qmcA"
                     /locus_tag="ACHOT6_003617"
     CDS             10188..11105
                     /gene="qmcA"
                     /locus_tag="ACHOT6_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFP9948775.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11102..11560
                     /gene="ybbJ"
                     /locus_tag="ACHOT6_003618"
     CDS             11102..11560
                     /gene="ybbJ"
                     /locus_tag="ACHOT6_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFP9948776.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11561..11968)
                     /gene="cueR"
                     /locus_tag="ACHOT6_003619"
     CDS             complement(11561..11968)
                     /gene="cueR"
                     /locus_tag="ACHOT6_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFP9948777.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(11965..12062)
                     /locus_tag="ACHOT6_003620"
                     /pseudo
     CDS             complement(11965..12062)
                     /locus_tag="ACHOT6_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(12093..>13284)
                     /gene="ybaT"
                     /locus_tag="ACHOT6_003621"
     CDS             complement(12093..>13284)
                     /gene="ybaT"
                     /locus_tag="ACHOT6_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFP9948778.1"
                     /translation="LGQAALLMEASTWVAFAFGGIVAMFSGYAYARLGASYPSNGGII
                     DFFRRGLGNGVFSLALSLLYLLTLAVSIAMVARAFGAYAVQFLHEGSQEEHLILLYAL
                     GIIAVMTLFNSLSNHAVGRLEVILVGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGA
                     FFSCIGITFLAYAGFGMMANAADKVKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDV
                     SALELEKYADTAVAQAASPLLGHVGYVIVVIGALLATASAINANLFAVFNIMDNMGSE
                     RELPKLMNKSLWRQSTWGNIIVVVLIMLMTAALNLGSLASVASATFLICYLAVFVVAI
                     RLRHDIHASLPILIVGTLVMLLVIVGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKR
                     NKTV"
BASE COUNT         3031 a         3856 c         3468 g         2929 t
ORIGIN      
        1 ctctccagcc tgcccgtggc actgtagccg tattcccgcg tctgtcgcgg gccgctgatg
       61 cgcaccaggt cgccgttgtc attccacccg tagtcacggt catacaccag gctgttcagg
      121 tgctggctct gtaactggcc tgcgggggtg tatgtgctgg tcagttcata tgcggcatta
      181 ctgcctgccc tgctgccgaa gctgcgcacc gtctcacggt gcagcctgtc ccgcgtatac
      241 tcgaccagcg gcgtgtcgcc aagcttcatg cccgccagat aaccgctgcc gtaggtcagc
      301 cactccaccg gcggcaggct gtccggcgtg acgcggtttg ccagcccctg ttcgttgtag
      361 gcgtgtgttg tctcatgctg ccacagcagt tcccccgtct ccgggttctc caccgtctgc
      421 cgttcgccgg tcaggcggcc cttatcgtcg tagccgtagt ggacagcgac acggtggcct
      481 tcgctcaggt ggctgatgtc tgtcagccag ccgtggtcat catactgcca ctgctctgcc
      541 ggttcgccgt tcaccgtgcg gtgagtgata cggtccgatt catcgtagta ccagagggtg
      601 acaagcccct catcttcact ctgcgtgagt tttccggtca ggtcataatg ataacgttgc
      661 gtccgcccgt caaagccgcc ctgctgtacc aaccggtcca gcgcatcgta gctgaagtcg
      721 ctgtggctgc cgttctcgtt agtcagactg atgacacgtc cggcagcatc gtactccata
      781 ctgcgcgtca gcccgccctg cgtggtgctg accgcctttc cccacgcatc gtactgtgtc
      841 tcgctccggt tgccgtccgg ggtgataacg gcagtcaggt cgcctgcggc gttgtactca
      901 tactgcgttt cacggccctg tgcgtctttc accgaggtta accggccacg gttgtcatag
      961 tggcggtaaa ggctgatacc ttcctcgcgg tgaaccgccg tcatctggcc gaagcggtcg
     1021 tattcatagc gggtctggta gcccgagcag tcagtgaacg ccagcaactg cccgtagcgg
     1081 ctccacgtca tctgccgggt gctgcccgtc gcatccgttg tcgtcgccgg taactcactg
     1141 tgcgcgtcat cgtagcggta gcgtactgtc tccccgctgc gcgatgtctc cgataccagc
     1201 ctgcccggtt catcatattc ccggcggctc tccagcccgt ccgggtacac caccgccgtc
     1261 agctggttcc cgtcgttata gtaaaatttc gtctcccgcc cgtccggtgt ggtgatgtcc
     1321 gtgatatcgc cggacaccac attcagaccg tactctgtcc gccgtcccgc cgcgtccgtc
     1381 tgcgccgtga gccttcctgc cgcgtcatac ccgctgtgcg tgacgctgcc gtccgccagt
     1441 tcttttttca ccacccgctt cagcccggcc ccgccttctg tatgcagcac ctcacgccgg
     1501 ttcaggctgt cggtgacggt gatgcggtcc tgctcatact ggtagcggta actcaggcct
     1561 gccgggttaa gttgctccac cacccgcccc gcatcgtcgt agcggtagcg catctccggc
     1621 ctgcctgcat aacggtgccc caccatccgg ccccggtatt tatcatcgta agtaaagtgg
     1681 cgcatctgcg taccgctgcg gtcatacacc gccgccagct caccgcgtgg cgtccagtcg
     1741 tagcacacca gtggcgcggc aggtaaattt tccgggtatt ctgggtcgtg catcagccac
     1801 accgccgaaa gacggatacc gctgtcagcg ccgtattccg taccgggcag tgtgtcgggg
     1861 aaggaaggag ctgagagagg gcggggactg tcaggggaag ataacgaagc ggtgtgcggt
     1921 ttacgggcct cttccgcccg ctgtgcctgt gtggtcagta ccagacggaa ctcccgcccg
     1981 gcaccgtccg tcacgccggt gatttccccg gccaggtcac cggcggcctc acgccggtac
     2041 gccagcgtcc gcccgaagcg gtccgccatc ccggtaagca cccggtacgg cggcagcggc
     2101 gctggcagta agtcctcagc acccggcacc cgctcagacc accccagtat ccaccacggc
     2161 ccctgtgcgc tgttggtcgc caggtaaaga tgcgggctta accggatatc cggtggcagc
     2221 gacgcccaca gccgtgccag cgtgtggccg tccggctgcg ctgccttacc gccacgcacc
     2281 agccacattg actcactgcg gctgtacacc gcctcccccg gcagcagcgg ctcaaagtga
     2341 atgctccgcc cgccgttgtc gttgagtatc agtgcatcat cgcgtatctg taagcggata
     2401 tcagaaggcg ctttccagcc ggggccgaaa acgcccaccg gtgcaggcgt cttcgtccgg
     2461 tagctgctgt aggtgcggga gagaatgaac ggcagcgggc cgggcagcgc aaggtccgtc
     2521 tcgccgggct gtacttttgc accgagcagc gggttcaccg ggttgcccga ggtcatcccg
     2581 cccggacaca ccgagcacgc cacgccagtt ggcgcgccaa ttcttacacc tgccgccccc
     2641 tggacaatgg gaccgccata ctgagtcata tctccctgac gcgccgctgg ttttccgctc
     2701 atataaagtg cccttttagt tattgttcat atccctgtgt tagcaacaaa aaaaataaaa
     2761 tgacacacaa gaccagctaa tgtatttata tatttttcac ataaacttat ttgttctcaa
     2821 tcaacaacaa ctgttattac aggtcacttt caatatttta ttaaactaaa atttttagca
     2881 cagatgaatt catcatcaac aaattcacta tatggataat atccattcaa cgcatatgat
     2941 ttatgatttt attagtctga gttatttttg tagggctata tttaaaatta ttaaactcca
     3001 ccgattcaga ttaatatcgc aatagaaaga taggaaatca ccaattcaat aacatcatca
     3061 atgaaacata ttgtagaatt aatttaaaca aggcgttcca ttcaaccaga acgcccctta
     3121 tttactctca tcaccccgca aactgcctga acagcgcctt acccttaacc agtcgcgcac
     3181 ccagccagcc gccgaaaagc gacagcagca gcgcaccgct gcacggcaga acaatccaca
     3241 atcgccagtc tggctcccac gggaaatcaa acactttcgc ctgcaacact gccagtgccg
     3301 tttccgcacc aattgcggcc accaggccgg aaacaaaccc aagcatggcg aactcacacc
     3361 acaatgtcgt gcgcagcagt tttttccccg caccgagtgt gcgccatacc accagctcct
     3421 gatgacgctg gcgcattccc acctgcacct gtgccagcaa cagcaacata ccgcaggcgg
     3481 tgaccagcac caccatcacc tccagcgccc gacttacctg ctccagcacc tgaccgacct
     3541 gttttaaaat cgcgccaata tctaacaggc taatggtcgg gaactggcgg ttgagttgcg
     3601 tcaacatgcc gttgccattc tcccagcgga aactggtaag ccagctctgc ggttgcccgt
     3661 ctaatgcccc ttccgggaaa ataaaataga aattaggccg cagactttcc cagtccactt
     3721 tgcgcaggct ggtcacttta gcgcggaact cctgggtatc gcccataaaa gtcacggtat
     3781 caccgagggc aacgtttaag cgtttcgcca gcccctcttc catcgacact tcatcggctt
     3841 ttggcggcca gttaccggcg acaatcggat tatgatcggg ccgcgtattt tgccaggtaa
     3901 gattgagttc gcgattgagc gcctcatctt cattgccttc tgtcggcttg ccgttaatcg
     3961 ccgtcagtct cgcccgcacc accggataaa acgattccgg gactatctga tgttccgcga
     4021 ggaacgcttt tagcggtgct acctgttctg tggcgatgtt aattaaaaag tagttcgggc
     4081 tttctggcgg tagctgctgc tgccagcggt cgagcagatc gccacgcaac accagcagca
     4141 gtgccagcag cataaaggag agcgaaaatg ccgaaagctg gcttaacgtt gaccacggct
     4201 gacgtaacag gcggctaacc gccaggcgca gaggcagcga tttcagcgtc atgcggcgaa
     4261 gtacattcag cagcatccag cccagcacac cgcacagcaa agccagtact accgcgcccg
     4321 ccagcaccgc ccaaagcagc atactgccac ccattaatcc ggcgagcagc agcacaacca
     4381 ccacactgac aatcggcaga taaaacttca gcggccaaac gttcgctacc acatcattac
     4441 gtaatacgcg taaaggctgc gttgccagca acaagcggta cggtcgtagc cccaccagca
     4501 gcgagatgac cgtcatggtg ccaagcgccc acagccacgg ccagaggctg gctggcggta
     4561 gtgcagcagg cagaacgggc ttgagcagca ccatcaacac gttttcgaac aacaggccta
     4621 tcgccccacc ggtaatggct gaaagcgtca gcaccatcaa ccactgaccg acgattagct
     4681 tacgcagttg cgctcgccct gcccccagcg ttttgaggat cgccaccaga tcgtagcgac
     4741 tgcgacagta atgattcatc gctaccgcca ctgctgccac tgccagcagc aaggtcagaa
     4801 gcgccgaaag cagcaggaac tgctgcgagc gttccatcga tcgccccagc gcgccttcgt
     4861 cctgttccag accgtaccag cgttgttcgg gtttaagctg gggtaacaac catttctcat
     4921 agccgtcgag ctggttctcg ttgccgccga atttataacg ccaggtgacc cgactccccg
     4981 gctgcacggc tccggtttta tcgacatccg ccagattcat catcagacgc ggagccatct
     5041 ggaaggggtt aaaaccggaa tccggttcct gaatcacttc tccggcaatc cgcaaggtgg
     5101 catcgcccac gtcaatggtg tcgcccgttt tcaggttaag cagtgccatc aggcgtgggg
     5161 ccagcaatac gctgcccgcc tgcggtttca ggccaggggg attggtttgc agatcgccat
     5221 acatcgggta gatatcatcc accgctttga cgttcgccag ctgcggtgtg tcgcctgcaa
     5281 atgtcatggt ggcgaaagtc agctgcttgc cgactttcag gccgcgcttt tgcgcttcct
     5341 ccagccatgc ttgcggcact tcgcgcgaac tgcgcaacgc ccgatcgccc gccataaact
     5401 cccggctttg ttggcttaag cccttctcca tgcgatcgct gatattgccc agcgccagca
     5461 cgcaggccac cgccaggctt agcgccagcc agacaattaa tagcgacggc gaacgccatt
     5521 cgcgccagaa ccaacgtgca atcatgcttc ctcctgcaac tgcccgttca ccagccgtaa
     5581 gcagcggtcg cagcgtgccg ccagttgcag gtcgtgggtc accataatca acgtggtgcc
     5641 atgctcacgg ttgagggaaa acagcaagtc ggcaatttta tcgcccgtct ggcggtcaag
     5701 gttgccggtg ggttcgtcgg caaacagcac atcaggtcga ccattaaagg ctcgcgccag
     5761 cgccactcgt tgctgttcgc cgccggaaag ctgtgccgga agatgatcaa gacgtttacc
     5821 cagccctaac tgttcgagca acgctttcgc tccgttacga ctttccgcgc tactctcacc
     5881 gcgcagcagc gccggaagct cgacgttttc cagcgcgtta agggtaggaa ttaacataaa
     5941 tgactgaaaa acaaagccga cgtgcttcgc gcgcaacttt gcccgcgctt cttcgtccat
     6001 attatgtagc ggttgtccca ccagactcac ttcgccactg ctgccgtcat ccagcccggc
     6061 gaggatcgcc agcaaggttg acttacccga tcccgactcg cccaccagtg cgatggtctc
     6121 gccacgtttg acaaccagct caactccggt gaggatggag agttcatgct ccccctgacc
     6181 gacggacttc ttaagatgat gaacttcaac aatgttttcc gctggcattt gcccttcctg
     6241 ttcctggtcc tgttaacctt ccgtgccgcc gcagcggaca cgttattgat tctgggtgat
     6301 agcctgagcg ccgggtatcg aatgtctgcc agcgcggcct ggcctgcctt gttgaatgat
     6361 aagtggcaga gtaaaacgtc ggtagttaat gccagcatca gcggcgacac ctcgcaacaa
     6421 ggactggcgc gccttccggc tctgctgaaa cagcatcagc cgcgttgggt gctggttgaa
     6481 ctgggcggca atgacggttt gcgtggtttt cagccacagc aaaccgagca aacgctgcgc
     6541 cagattttgc aggatgtcaa agccgccaac gctgaaccat tgttaatgca aatacgtctg
     6601 cctgcaaact atggtcgccg ttataatgaa gcctttagcg ccatttaccc caaactcgcc
     6661 aaagagtttg atgttccgct gctgcccttt tttatggaag aggtctacct caagccacaa
     6721 tggatgcagg atgacggtat tcatcccaac cgcgacgccc agccgtttat tgccgactgg
     6781 atggcgaagc agttgcagcc tttagtaaat catgactcat aaagcaacgg agatcctgac
     6841 aggtaaagtt atgcaaaaat cggtcttaat taccggatgt tccagtggaa ttggcctgga
     6901 aagcgcgctc gaattaaaac gccagggttt tcatgtgctg gcaggttgcc gaaaaccgga
     6961 tgatgttaag cgtatgaaca acatgggatt taccggcgtg ttgatcgatc tggattcacc
     7021 agaaagtgtt gatcgcgcag cagacgaggt gagcgccctg accgataatt gtctgtatgg
     7081 gatctttaac aatgccggat tcggcatgta tggccccctt tccaccatca gccgtgcgca
     7141 gatggaacag cagttttccg ccaacttttt cggcgcacac cagctcacca tgcgcctgtt
     7201 acccgcgatg ttaccgcacg gtgaagggcg tattgtgatg acatcatcgg tgatgggatt
     7261 aatctccacg ccgggtcgtg gcgcttacgc ggccagtaaa tatgcgctgg aggcgtggtc
     7321 agacgcactg cgcatggagc tgcgccacag cggaattaaa gtcagcctga tcgaacccgg
     7381 tcccattcgt actcgcttca ccgacaacgt caaccagacg caaagtgata aaccagtcga
     7441 aaatcccggc atcgccgccc gctttacgtt gggaccggaa gcggtggtgg acaaagtacg
     7501 ccatgctttt attagcgaga agccgaagat gcgctatccg gtaacgctgg tgacctgggc
     7561 agtaatggtg ctcaagcgcc tgctgccggg gcgcgtgatg gacaaaatat tgcaggggtg
     7621 agttgaagcg cgcgcttaag cccccatgtc aaagaaaata tcgacaacag agagcgactc
     7681 catgtccgta gaaaatattg tcaacattaa cgaatctaac ctgcaacagg ttcttgaaca
     7741 gtcgatgacc actccggtgc tgttctattt ttggtctgaa cgtagccagc actgtttgca
     7801 gttaaccccg atcctggaaa gcctcgcggc gcagtacaac gggcaattta ttctggcgaa
     7861 gctggactgc gacgcggagc agatgattgc cgcccagttt ggtctgcgcg cgattccgac
     7921 cgtgtatctg ttccagaacg ggcaaccggt agatggcttc caggggccgc aaccggaaga
     7981 ggcgatccgc gccctgctgg ataaagtgct gccgcgcgaa gaagagctga aagcgcagca
     8041 ggcgatgcaa ctgatgcagg aaggcaatta caccgatgcc ctgccattgc tgaaagacgc
     8101 ctggcagttg tcgaatcaga acggggagat cggcctgctg ctggcagaaa cgctgattgc
     8161 gctgaaccgt tctgaagatg cggaagcggt gctgaaaacc attccgttgc aggatcagga
     8221 cacccgctac caggggctgg tggcgcaaat cgaactgctg aagcaggcgg ctgatacgcc
     8281 ggaaattcaa cagttgcaac agcaggtggc ggagaatcca gaagatgccg cactggcgac
     8341 gcaactggct ctgcaactgc accaggttgg gcgcaatgaa gaggcgctgg agttgctgtt
     8401 cgggcatctg cgtaaagatc tcaccgccgc agacggtcag acgcgtaaaa cgtttcagga
     8461 gatcctcgct gcgctgggta cgggtgatgc actggcgtcg aagtatcgcc gccagctgta
     8521 tgcattgttg tattgatatt ccgttgatcg gcacatgaac cacaacgccg gatgcgatgc
     8581 tgcgcatccg gcatcgcatc atttcttctt caattgcgtc accaccaact ggtggcgcga
     8641 attataaaac ttacgatagg ttaaatagca ggcaataatg gtcgacaagc tggcggttga
     8701 gagcagcata aaggtcacca taatctgata tttaatcgcc ttcaccggat caatcccggc
     8761 aaatatcagc ccggacatca ttccaggtaa actcactaag ccaaccgttt ttgctgaatc
     8821 gaccgtcgga attaaagccg cgcgaatact gtcgcgaatc aatatcgctg aagcctgctt
     8881 cggcgtcgca ccaagactca gtttctcctg aatctgttgc tgttcgctaa tgacccgttg
     8941 ccctaaattg ttgtaacaca accccaccgc taccatggcg ttaccggcaa tcatcccggc
     9001 gatagggatc acctgcatcg ggataaattc aatcgaccca gagagaatca gcaccgccag
     9061 ggtaattccc gccccgaccg taatggcaat aaacgatgag ataaaagctt tagcaatata
     9121 tttactgcgt ttttgcgcgt tccacgccgc attaaagcag ataaataaca ccatcaataa
     9181 tgtcaggctg gcatcatcca cgctgaaaat atacttcagc acatagccga caataatcag
     9241 ctgaattatc gctcgcccga cgctccagaa aatatctttc tccagcgcca gtttttcttt
     9301 atggctaatt aagattgcca ccaccaccag cattaatgcc agtgccaatg attcgttagt
     9361 aatattatgc gagttcatag cgtgcttcct gcatttctcc ggcatgcggt tgcagtgtaa
     9421 tcactttatc cgcatgatta atttcgtctt tatcgtgtgt cacccacagc acggcaatat
     9481 tttgctcgcg cacataacga tggatcatct cattaacgtt atgtttatta ctttcatcca
     9541 gcgcactggt gatttcatcc agcaataaaa ccttcggcat aaattgcagg ttacgaatca
     9601 gtgagatgcg ttgtttttca ccaccagata gctcggcgat atttttcgtc aaaatgctgt
     9661 ccggcaaagc gaagcgttcg agaaaatcga gaaaaatggc cgggtcaggc tgctggttac
     9721 ggatctgcca gggaaagatc agattatcgt ataccgtatc gccaaacagc gtcggcgtct
     9781 gggcgcagta agagacttgc tggcggtaga tttctggctt tagtgtgctg acatcctcac
     9841 cttcaaacag taacgttccg ctggttgggc tgatcaatga agcaactatt tttagcagcg
     9901 tacttttgcc acaaccggaa ggaccggtaa ttaacttaaa ttcgccagca cgcagcgaaa
     9961 aattgatgtt attaagaatc ttcgcatcac ccgccagata tcctacgttt tgtagctgaa
    10021 gcaaaggact attttcctgc atcactgttc cctttttctg atttttacta aaaacagttt
    10081 atccttcgca ggaataaggg ggaactctct ttcagtaatc aggtataatt tgtttaatca
    10141 agcagcaatt ttgctgatta tggacggtac aacaggaggt ttttccgatg cttatcttta
    10201 tcccgattct catttttgtc gcgctggtca ttgtcggcgc gggcgtcaaa atcgtgccgc
    10261 agggctatca atggacggta gaacgttttg gtcgctatac caaaacgtta cagccggggc
    10321 tcagtctggt ggtgccgttt atggatcgca ttggtcgcaa gatcaatatg atggagcaag
    10381 tgctcgatat cccttcccag gaagttatct cgaaagataa cgccaacgtt accatcgacg
    10441 cagtctgttt tattcaggtg attgacgcgc cgcgcgcggc ttatgaagtc agcaatctgg
    10501 agctggcgat catcaacctg accatgacta acatccgtac cgtgttgggt tcaatggaac
    10561 ttgacgaaat gctctctcag cgcgacagca tcaactcacg cctgctgcgt attgtcgatg
    10621 aagccaccaa cccgtggggg attaaagtca cccgtattga aattcgcgac gtgcgcccac
    10681 cggcagagct tatctcttca atgaacgcgc agatgaaagc ggaacgtacc aaacgcgctt
    10741 acattcttga agcggaaggg atccgtcagg cggaaatcct caaagccgaa ggtgaaaaac
    10801 agtcgcaaat cctgaaagcg gaaggcgaac gtcagtcggc gtttttacag gctgaagcgc
    10861 gtgaacgttc cgccgaagca gaagcccgcg ccaccaaaat ggtgtctgaa gctatcgcct
    10921 ccggtgatat tcaggcggtg aactacttcg tagcgcagaa atacaccgaa gcgttacagc
    10981 agatcggttc ctccagtaac agcaaagtag tgatgatgcc attagaggcc agcagcctga
    11041 tggggtcgat tgccgggatt gccgagctgg tgaaagacag cgccaacaaa cggactcagc
    11101 catgatggag ttaatggtcg ttcatccaca tattttctgg ctcagtctcg gcggtttgct
    11161 gctggcagcc gagatgctgg gcggaaatgg ttatttgttg tggagcggcg tggcagcggt
    11221 gattaccggc ctggtggtct ggctggtgcc gctgggttgg gagtggcaag gggtaatgtt
    11281 tgccatcctg acgctgctcg ccgcctggct gtggtggaaa tggttgtcgc ggcgggtgcg
    11341 cgaacaaaag cacagcgata gtcatttaaa ccagcgcggg cagcagctga ttggccgacg
    11401 ttttgtgctg gaatctccgc tggtcaacgg gcgcggtcat atgcgcgtcg gtgacagttc
    11461 atggcctgtc agcgccagcg aggatctcgg tgcaggtacg catgttgaag tcattgcgat
    11521 agaagggata accctacata tccgagccgt ctcgtcttaa tcaccctgcc cgatgatgac
    11581 agcagccgga gagattttcg ataatcgggc agtcggcgct gtcatcgcca gggcaggcat
    11641 tcgccagtgc cagcagctgg tcgcgcatgg attgcagctc ctcaatgtgt cgttcgatct
    11701 ccgccacctt ctccagcgtg cgccgtttga cgtcggcgct gtgccgctgc gggtcgttaa
    11761 acagattcac cagctcgccg ctctcttcca ggttaaagcc cacctgccgt gcctggcgca
    11821 gtaaggtcag ttcgttgaga tgctgctgcg tgtaggtgcg ataaccgttt tcgctgcgca
    11881 tcggcggcgt caccagcccc ttctcttcat agaagcgaat ggctttgctg gtcaggccgg
    11941 taatttttgc tacatcgctg atgttcatcg ttcgcgcaac gcctcctgtg ctttgttaaa
    12001 gggtttcaac agatattcaa gcacgttttt ttgccagtgc gaatatcgac cgccaccaca
    12061 ataatacagg aagtactgcg cacagagaga tgttatacgg ttttattgcg cttcatgacc
    12121 attgccacaa taaggctgag taagagtgag ccaataatcc atatcaacgc acggctaccc
    12181 tgggaccaca gactgtagat aaagccaacg atcaccaaca acatcaccaa cgtaccaacg
    12241 ataagaatcg gcaacgaggc gtgaatatca tgacgcaggc ggatcgccac cacaaacacc
    12301 gccaggtagc aaatcaaaaa ggtggcgctg gcaacgctgg cgagtgagcc taaattcagt
    12361 gccgccgtca tcagcataat caacaccacg acaatgatgt tgccccaggt actctgccgc
    12421 cacagggatt tattcattag cttcggtagt tcgcgttcgc tgcccatgtt gtccatgatg
    12481 ttaaacacgg cgaacaggtt cgcgttaatg gctgaagccg tcgccagtaa agcgccgatg
    12541 acgacgatca cataacccac atgcccgagc agcggagaag ctgcctgcgc tacggcggta
    12601 tcggcatatt tttctaactc taatgccgat acatcgctaa gcaaaaccag cgccagcgag
    12661 atataaagca acgtggtaac gccaattgcc accagaaacg cccgtggcat aatgacctgc
    12721 ggatctttca ctttatccgc cgcgttcgcc atcatgccaa agcccgcata ggcaaggaag
    12781 gttatcccga tacaggagaa gaacgcaccg gagctggggg gcgcagagac agaaatatgc
    12841 gccggttgca acgaccagac accggcaata atcagcaata acaggatcat cattttaatg
    12901 ccgacgagga tcacttccag ccgccctacc gcatggttgc ttaaggagtt gaaaagcgtc
    12961 atcaccgcaa tgatccccaa cgcgtagagc aaaataagat gctcctcctg gctgccttca
    13021 tgcaaaaact gcacggcata agcgccaaaa gcacgggcga ccatggcgat gctcaccgcc
    13081 agcgtcaaca ggtacagtaa cgagagcgcc agcgaaaaga cgccgttgcc taatccgcga
    13141 cgaaagaagt cgataatgcc gccattgctg ggatagctcg cccccagacg cgcataggca
    13201 taaccggaaa acatcgccac aataccgcca aaagcaaagg cgacccaggt cgaggcttcc
    13261 attagcaacg cagcctgccc cagc
//
LOCUS       DAZKIV010000129        13199 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZKIV010000129 DAZKIV010000000
VERSION     DAZKIV010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13199
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.129"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..226)
                     /locus_tag="ACHOT6_003622"
     CDS             complement(<1..226)
                     /locus_tag="ACHOT6_003622"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948779.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHSKPLEIIKT"
     gene            163..1662
                     /gene="pitB"
                     /locus_tag="ACHOT6_003623"
     CDS             163..1662
                     /gene="pitB"
                     /locus_tag="ACHOT6_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFP9948780.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTLTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1716..2408)
                     /gene="yghT"
                     /locus_tag="ACHOT6_003624"
     CDS             complement(1716..2408)
                     /gene="yghT"
                     /locus_tag="ACHOT6_003624"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9948781.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGSYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLIDAEK
                     AITDFMTARGYH"
     gene            2582..3295
                     /gene="yghS"
                     /locus_tag="ACHOT6_003625"
     CDS             2582..3295
                     /gene="yghS"
                     /locus_tag="ACHOT6_003625"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFP9948782.1"
                     /translation="MNKRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3327..4085
                     /locus_tag="ACHOT6_003626"
     CDS             3327..4085
                     /locus_tag="ACHOT6_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948783.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4131..5480
                     /locus_tag="ACHOT6_003627"
                     /pseudo
     CDS             4131..5480
                     /locus_tag="ACHOT6_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
     gene            5480..6304
                     /locus_tag="ACHOT6_003628"
     CDS             5480..6304
                     /locus_tag="ACHOT6_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948784.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6316..6876
                     /locus_tag="ACHOT6_003629"
     CDS             6316..6876
                     /locus_tag="ACHOT6_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFP9948785.1"
                     /translation="MSFRLIIALIIMLLAPGGQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6907..7977
                     /gene="lptF"
                     /locus_tag="ACHOT6_003630"
     CDS             6907..7977
                     /gene="lptF"
                     /locus_tag="ACHOT6_003630"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFP9948786.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLVQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7974..9053
                     /gene="lptG"
                     /locus_tag="ACHOT6_003631"
     CDS             7974..9053
                     /gene="lptG"
                     /locus_tag="ACHOT6_003631"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFP9948787.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFTAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9089..10261)
                     /locus_tag="ACHOT6_003632"
     CDS             complement(9089..10261)
                     /locus_tag="ACHOT6_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFP9948788.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10261..10509)
                     /locus_tag="ACHOT6_003633"
     CDS             complement(10261..10509)
                     /locus_tag="ACHOT6_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFP9948789.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10541..11455)
                     /locus_tag="ACHOT6_003634"
     CDS             complement(10541..11455)
                     /locus_tag="ACHOT6_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFP9948790.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESCF
                     CQRFLFISSLAARHPELSWYANSKHIAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11452..13140)
                     /locus_tag="ACHOT6_003635"
     CDS             complement(11452..13140)
                     /locus_tag="ACHOT6_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFP9948791.1"
                     /translation="MRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEYQTLKTR
                     AEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGVGQRDSW
                     SAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADVALQRPV
                     PNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLPFYHDMG
                     LVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKFISKNRGTVSVAPPFGYELCQRRVNE
                     KDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFMPCYGLAENALAVSF
                     SDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRNEAGMPV
                     AERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTGRIKDLI
                     IIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRGQLIHAL
                     AARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLNVQESLA"
BASE COUNT         3165 a         3254 c         3426 g         3354 t
ORIGIN      
        1 gtgttttgat tatttctaaa ggttttgaat gaatgcttat tgtctgatac acaagaaata
       61 acactctttt tatcgttaaa aaatgatatt tcactttgcc catgccgtta aagtcactga
      121 taatgcgtcc gttcgtaaat tcaaaatggc gtaatctaat atatgctaaa tttatttgtt
      181 ggccttgata tatacacagg gcttttgtta ttgcttgctc tggcatttgt gttgttctac
      241 gaagcaatca atggttttca tgacacggcg aatgcggtgg caaccgttat ttatactcgt
      301 gccatgcaac cacaacttgc tgtggtgatg gcggcatttt ttaacttttt tggcgtgtta
      361 ttgggcggac ttagcgttgc ctatgccatt gtccatatgt taccaaccga tttgttgctg
      421 aatatggggt caacccacgg tctggcgatg gtcttttcca tgctgctggc ggcgattatc
      481 tggaacctgg gaacgtggtt cttcggttta ccggcctcca gttcgcacac cttgattggt
      541 gcgattatcg gcatcggttt aaccaacgcg ctgttaaccg gctcatcggt gatggatgcg
      601 ttaaacctgc gtgaagtgac caaaattttc tcctcgctga ttgtttcccc tatcgtcggc
      661 ctggtcattg cgggaggcct gatattcctg ctgcgacgct actggagcgg gacgaaaaag
      721 cgtgaccgta ttcaccgcat tccggaagat cgcaaaaaga aaaaaggcaa acgtaaaccg
      781 ccattctgga cgcgtattgc gctgattgtt tccgctgcgg gcgtggcgtt ttcgcacggc
      841 gcgaacgacg gacaaaaagg gatcggcctg gtaatgctgg tactggtggg gattgcccct
      901 gctggcttcg tcgtcaatat gaatgcgtcc ggctatgaaa ttacccgtac ccgcgatgcc
      961 gttaccaact tcgaacacta cctgcaacag catcctgaac tgccgcagaa gttgattgcg
     1021 atggaacctc cattgcctgc agcatcgact gatggcacgc aagtaacaga gtttcactgt
     1081 catccggcaa atacctttga tgcgattgcg cgcgttaaaa cgatgctgcc aggcaatatg
     1141 gaaagttacg agccgttaag cgtgagtcag cgcagccagc tgcgccgcat tatgctgtgc
     1201 atctctgata cctccgcgaa gctggcgaaa ctgccaggcg tcagtaaaga agaccagaac
     1261 ctgctgaaaa aactgcgcag cgatatgtta agcaccattg agtacgctcc ggtgtggatc
     1321 atcatggcgg tagcactggc gctcggcatt ggcaccatga ttggctggcg tcgtgtagcg
     1381 atgaccatcg gtgagaagat tggtaagcgc ggcatgacgt atgcgcaagg catggcggca
     1441 caaatgacgg cggcagtgtc tatcggcctt gccagttata ttgggatgcc tgtctccaca
     1501 acacacgtcc tctcgtctgc ggtagcaggg acgatggtgg tggacggcgg tgggttacag
     1561 cgtaaaacgc taaccagcat cctgatggcg tgggtgttta ctttaccggc ggcaattttt
     1621 ctttctggtg ggctgtactg gatagcattg cagttgattt aaccttcctg aaaatgcccg
     1681 gtcctggcga tcggatcggg cagttccatt tttgactagt gataaccacg cgcggtcata
     1741 aaatccgtaa tcgctttttc tgcatcaatc agcacctgtt ccagtggctg attggcatcg
     1801 atatcaacca gttgtgcacc accaaaggtt aactgtggcg ttatggcaat cttcctcgcc
     1861 agcgattccc gtttatggtc gggtttacgc gcgcaggcaa cttcaaggtc aacattgagt
     1921 ttgatgacca gatcaggctt atggctcgcc atccagtgaa acgctttacg ttcctggctt
     1981 gccagccatg agacaaaacg accaccttca acgttaggtg ggaacaccgt accatcgtaa
     2041 gagccaggaa tttggtcctg aggataacgg tcggttagaa caattaaccc gcgacgacga
     2101 caggcaagca tatgacgaaa gcgcagtaag cgacgggcga caaacgctgt aattaccagc
     2161 gccggaactg gtcctggcaa tttttttgct gttttcacct gatttcgttc aattgtttta
     2221 tgtaaggatt ttcccatcaa cggtaatttt gtcactgcac gaccgacatt tccggcttgt
     2281 tttcctaaat gaactctttc ggcagcacca tatttttcga caacggtaat aagatgttca
     2341 cacaccgttg acttgcctga accatcgcta ccaataacgg caattaatgg aggtgttatt
     2401 gactgcatga atatatcctt taatttaaat atccattaaa aatatttatt tggttaatat
     2461 gtttttatga aagcgtaatt caggtcaatg tcacaattaa ccattgtcac aataaggttg
     2521 aacggatatt ctgagcacgg accacgtaat attcaataca ttatttactg ccgggttttt
     2581 cgtgaataaa cgtaatatgt caataattaa ttccacgcca gtgcgtgtta ttgccattgt
     2641 aggatgtgat ggttcaggta aatcgaccct cacggcaagc ctggtaaatg aactggcagc
     2701 aagaatgcca acagaacaca tttatctcgg gcaatcgtcc gggcgaattg gcgaatggat
     2761 ttcacagctc cctgttattg gcgcaccttt tgggcgttat ctgcgaagta aagcggcaca
     2821 tgtgcacgaa aaaccctcaa caccgcctgg caatattact gctctggtta tctatctgct
     2881 ttcctgctgg cgggcgtaca agtttcgcaa aatgttgtgt aaaagccagc aaggctttct
     2941 gctcatcacc gaccgctacc cgcaggttga agtgccgggt tttcgctttg atggcccgca
     3001 attggcaaaa accacgggcg gtaacggttg gataaaaatg ttaaggcagc gcgagctgaa
     3061 gctgtaccaa tggatggcat cttatttgcc cgtattgttg attcgtcttg gcattgatga
     3121 acaaaccgcg tttgcgcgta aacctgacca ccaactggca gcgttacagg aaaaaatcgc
     3181 cgttacgccg caactgacat tcaatggcgc aaagatcctt gaactggatg ggcgacaccc
     3241 cgccgatgaa attctgcaag cgtcactacg cgcaattcac gccgcgcttt cctgatcacc
     3301 aaaatggata ggggatgatt taacgaatgg atgcactaca aactcaaact gttaatagta
     3361 caaccgcgcc gcagcccaac tacattccgg ggctgattgc ggtggtcggg tgtgatggca
     3421 ccggtaaatc cacactgacc accgacctgg tgaaatcgct gcaacaacac tggcaaaccg
     3481 agcggcgcta tctggggctg ctctccggcg aagacggcga caaaatcaaa cgattgccgt
     3541 tggttggcgt ctggctggaa cggcgactgg cggccaaatc ctcgaaaacc caaagcatga
     3601 aaaccaaatc tccggcgcta tgggcggcgg tgattatgta ctgcttctcg ctgcgaagaa
     3661 tggcgaatct acgcaaggtt cagcgactgg cgcaaagtgg cgttctggtg gtcagcgatc
     3721 gcttcccgca ggctgaaatt tcgggctttt attatgatgg accggggatt ggcgtcgaac
     3781 gtgcgaccgg gaaaatcagc atgtttctgg cgcagcgcga acggcgttta taccaacaaa
     3841 tggcgcaata tcgcccggaa ttaattattc gcctgggcat tgatattgag actgccatct
     3901 cccgcaagcc tgaccatgac tatgccgagc tgcaggacaa aatcggcgtc atgtcgacga
     3961 ttggctataa cggcacaaaa attcttgaga ttgattccag agcgccctac agcgaagtgc
     4021 tggaacaggc acagaaggcg gtttctctgg ttgccatcgt ttctgaccgc cgaagtttaa
     4081 cttaaggctg gcgggatact ttttactatc aacgataagg tcaggctgaa ttggcgggtt
     4141 ttaacatcaa acattggttt gcagatggcg cgtttcgcac cattattcgc aatagcgcct
     4201 gattaggctc cagtaatgtc gtgagcgcct tgctgggtct gttggcgctc tcgtgtgccg
     4261 gtaaagggat gacgcccgcc atgtttggcg tactggtgat tgtgcaatcg tacgccaagt
     4321 cgatcagcga ttttattaag tttcagacat ggcaactggt ggttcagtac ggaacaccag
     4381 cattaaccaa caataatccg cagcaattcc gcaatgtcgt ctcattttcc ttctcgctgg
     4441 atatcgtcag cggcgcggtg gcgattgtcg gtggcattgc cttactgcca ttcctttccc
     4501 attcattagg tctggatgac caaagttttt ggctggcagc gctctattgc acgctcattc
     4561 cttcaatggc ttcctccacg ccgaccggca ttctgcgtgc ggtagatcgc ttcgatttaa
     4621 ttgctgtaca gcaggcgacg aaaccttttc tgcgcgcagc ggggagcgtc gtagcctggt
     4681 attttgactt tggttttgcg ggttttgtta ttgcctggta cgtgtcgaat ctggttggcg
     4741 gcaccatgta ctggtggttt gccgcgcgcg aattacgccg ccgaaatatc cataatgcct
     4801 tcaaattgaa tctgtttgag tctgcccgac acatcaaagg cgcgtggagt tttgtctggt
     4861 caaccaacat tgcccactcc atctggtcgg cgcgtaactc gtgcagcaca gtgctggtgg
     4921 ggatcgtgtt aggacccgct gccgccgggt tatttaaaat cgccatgaca ttcttcgacg
     4981 ccgccggaac gccagcgggt ttgctgggta aaagttttta cccagaggtg atgcgtttag
     5041 atccgcgcac caccagaccg tggttgttag gtgtgaagtc cggtttactg gcgggcggaa
     5101 tcggtattct ggtggcgctc gcggtgttta tcgtcggcaa gccgctcatt tcgctggtgt
     5161 ttggcgttaa gtatctcgaa gcgtatgacc tgattcaggt gatgctgggc gcaattgtga
     5221 tttcgatgct gggcttccct caggagtcat tgctattgat ggcgggaaaa cagcgcgctt
     5281 ttctcgtggc gcaaaccatc gcctcaatcg gctacatcgt attgctgttc atgttctgtc
     5341 atctgtttgg tgtgctggga gcagcgtttg cttacttcgg tgggcaatgt ctggacgtgg
     5401 cgctctcgtt aattccaacc ttaaaggcat tttttcagcg ccattccttg ctttataacg
     5461 cagccggaga gaaatcctga tgatgagaaa atatttcccg cttgaggcga gcgagcgtct
     5521 atttgttgcg attgaagaag atgatgtcgt tgatgcacag gttagtttgc cgccgactat
     5581 tgcactaagt tgtaccaccg aaatcattca tgataattac gcgttatgcc tgcaattctg
     5641 gctcaatggc gttgatcgtc aggaattact gcgtttagtt cgcaaacaag ccaaaggcga
     5701 tgagctgact gctgatgagc gcaaacaatt taaatacatg cgagcgcgct acaagcacct
     5761 gcgttttgcg caacggctgt atttaaagaa gcatcaggcc ggatttttat tcggcaaaac
     5821 gaccgttttt ctggggcgtt ttcaggacgg ctttcgtaac ggtaagaaaa atattgtgtc
     5881 gtattacggc aacctgttac ggatttattt aagctctccg gtctggtcgt tggtgaacta
     5941 ttcattacgc catagccagt tagaaagcgt aagcagtttt attgcctatc ggcaaaagca
     6001 aatgcatacc ctgaaagaga ttattgccaa accgcgacta accggcaggg aatttcatga
     6061 tgtgcgaaaa atcatcagcc aacaggtttc ttattacgac accttaagat cgcttgatcc
     6121 tgaaaataaa gaggcgttac agatttcccg tttcctcgcc gccattaatg gcctgatggg
     6181 agataaacac gacgacatgg tggcggatga tatggaaaac cgccagtcgt atgatgcacc
     6241 gttggcgctg gatagcgata ttcgtcagcg cttagagctg ctgatttcgc gttttccgct
     6301 atagccggag caatgatgtc atttcgtttg attattgcgt tgattatcat gctcttagcc
     6361 ccgggtgggc aggcacacaa tttcgtcact ggcaaaacgg ttacccctgt ttatatccag
     6421 gaaggcggcg agctgcttct gaatagcgac gacgaaattc actaccaaaa gtggaacagc
     6481 acacaattag cgggaaaagt ccgcattatt cagtatatcg ccgggcgtaa gtcggcgaag
     6541 aaaaagaact cactgttaat taaagcggtt gaagcggcaa actttccgca ggatcgtttt
     6601 cagcccacca ccattgtgaa caccgatgat gccatctttg gtactggcta ttttgttgtt
     6661 ggcaaaatcg aaaaaaataa acgccgctat ccgtgggcgc aattcattat tgacggcaat
     6721 ggacagggac gcgttgcctg gcgcttgccg gagcagagtt caaccattct ggtgctgaat
     6781 aaagccgggc aaattcagtg ggcaaaggac ggcagcctga cgccggaaga ggtagatcat
     6841 gtcattgcgc tggcgcagaa actgatcaat gaataaaaaa ttcaaaggat tgagggcgtt
     6901 agtgagatga agctggttga acactacatc atgcgtggta cgcgccgtct ggtgctgatt
     6961 atcgtcggtt ttctgatctt tattttcgcc agctactccg cacagcgtta tctgaccgaa
     7021 gcggcaaatg ggacattagc tcttgatgtt gtgctggata tcgttttcta caaagtgctg
     7081 attgcactgg agatgttgtt acctgttggt ctgtatgtgt cagttggcgt gacgctaggg
     7141 cagatgtata ccgactcgga aattaccgct atctctgcgg cggggggcag tccgggacgc
     7201 ttgtacaaag ccgttcttta tctggcgata ccgctaagta tttttgtcac ccttctgtcg
     7261 atgtatggtc gtccgtgggc ttatacgcag atttatcaac tggagcagca gtcgcagtcg
     7321 gagctggatg ttcgccagtt gcgggcaaag aaatttaaca ctaacgataa cggacgaatg
     7381 atcctttcgc agacggttga tcaggataat aatcgcctga ctgacgcgct gatttatact
     7441 tctactgcca atcgaacccg cattttccgc gcccgttcgg ttgatgtggt tgacccatca
     7501 cccgagaaac cgaccgttat gttgcacaac ggcactgcct atcttctcga tcatcagggg
     7561 cgtgacgaca acgaacagat ctaccgtaat ctgcaattac atctgaatcc gctggttcaa
     7621 agccctaacg tcaaacgcaa agcgaaatcg gtcacggagc tggcgcgctc cgtctttcct
     7681 gccgatcatg ccgaactgca atggcgacaa agccgtggtc tgacagcatt gttgatggcg
     7741 ctgctggcca tttcattaag tcgggtaaaa ccgcggcaag ggcgattttc aacgttattg
     7801 ccactgacgt tgctgtttat tgccattttt tatggcggcg acgtctgccg tacgctggtg
     7861 gctaacggcg cgattcccct cattcctggt ttgtggttag tgcccggact catgctaatg
     7921 ggcctgctga tgctggtcgc acgcgacttc tctttgctgc agaaattttc ccgatgaatg
     7981 ttttcagtcg ttatttaatc cgtcatctct ttctcggttt tgccgccgcc gcagggctat
     8041 tgctgccgct ttttaccacc ttcaacttga ttaacgaact ggatgatgtc agcccgggcg
     8101 gttatcgctg gactcaggcg gtgctggtgg tgctaatgac cttaccgcgc acactggtcg
     8161 aactttcgcc gtttatcgcg ttattgggtg gtattgttgg actggggcag ttatcgaaaa
     8221 acagtgagct taccgccatt cgcagcacgg ggttttctat cttccgtatt gcactggtgg
     8281 cgctggttgc cgggatattg tggactgttt cgttaggcgc gatagatgag tgggtggcgt
     8341 cgccattgca gcaacaggcg ttgcaaatca aatcgaccgc caccgcgttg ggggaggacg
     8401 atgacattac cggcaatatg ctgtgggcca ggcgtggtaa tgaatttgtg acggtgaaat
     8461 cgctgaacga gcagggccag ccggtgggcg tggagatatt tcattatcgc gacgatctct
     8521 cgcttgaatc ctacatttat gcacgcagtg ccaccattaa agacgacaaa acgtgggtcc
     8581 tgcatggtgt gaatcataaa aaatggctga atggcaaaga aacgctggaa acattagata
     8641 atcttgcctg gcaatcggcc ttcaccagta tgaatcttga agagttatca atgccgggga
     8701 atactttttc tgtccgtcag cttaatcatt acatccatta tttgcaggaa accgggcaac
     8761 ccagcagcga ataccgcctt gcactgtggg aaaaactggg gcaacccatc ctgaccctgg
     8821 cgatgatttt gctggctgtg ccgtttacct ttactgcccc gcgttcgcct gggatgggta
     8881 gccgtctcgc tgtaggcgtc atcgtgggct tactcacctg gatcagctat caaatcatgg
     8941 ttaatctggg attgctattt gcgttaagcg cacctgttac cgcgctcggt ttaccggtag
     9001 cgtttgtgtt ggtggcgttg agcctggtgt attggtatga cagacaacat taaacccgcc
     9061 gtggcgcaaa caccacggcg ggctcacatt actgcttcag agtagcaaat gcctgaatga
     9121 tggcatcgat ttgctcacga ctgtgggcag catttacgct acagcgcagc agcgtgatgc
     9181 ctgcaggcgc ggcaggcggc aggataagat tcacatacac gcccagcgag ataagctcgc
     9241 gccagatgcg taaaccttcc tctttcgaac cgattattac tggcactacc gggctaatat
     9301 gtgaacttaa ctcataaccc agtttttgca gaccgtcata cagatggttg gcgttatcca
     9361 tcagcttttg ccgcagctct ggttgtgtgg caatagttct taacgacgag cgcacggtcg
     9421 caatgcagga cggagaagga gaggcggtaa aaatatacgg gcggctgccg tagcgcagca
     9481 cttccatcgc ttccgaaccg acagcaaaac cgccaatcga ggccagactt ttactgaaag
     9541 taccaacgat aatatccaca tcatcttcaa cgccgacagc ttcagccagt ccgcgccctg
     9601 ttgcgcctaa cacgccaaac gaatgcgctt catcgacaat caaataacca ccgagacgac
     9661 gtttgatatc aacaatttcc gccagcggcg caacgtcacc gagcatgctg tagatgccct
     9721 caacgatgat aatcgcctct ttcgcccgtt caccgaggcg aaccatgcgc cgttccagat
     9781 ctttagcatc gttatggcgg aaacgaataa tttccgcgcc accgagagaa caggcgtcat
     9841 aaatactggc gtggctgtcg gcatccagta acaccacggc gttatggtcg gcaagtgcgc
     9901 tgatgacgcc taaattagcg gtataaccgg ttgaaaaaac gatagcggta gggcgattga
     9961 aaaactcggc aatctctttc tctaacgcca ggtgcggcgc ataactgccg tttgccatac
    10021 gagaaccggt ggtgccggtg ccttgggccg ctaacgctgc ctgaccttcg ctgatggcat
    10081 catgattaaa ggttaaaccg agataattat tggtcccggc cagaatcact tttttattgc
    10141 cgatccggcc ttcggtggcg gaatacactt catcaataca ggtgccaaac ggtgttaacc
    10201 cgttgtcact aaattgcagt cgctcgccag caaggcgcgc atatttatcg tatagcccca
    10261 ttatttattc tccagccatg gaagtagagt ttccattaat tgcgcagggg ttttgacatc
    10321 cagcagaata ttaataggaa tggaaatatc gaatctgtct tccagcatca ttaagagatc
    10381 catcactttg atcgactcca ggccaagatc gttgacgaga tcgctgtcgg gcttaatttc
    10441 cacaccgttt tcgaccaaat cctgtaaaca ggaaaggatg taatccatta ctatttcacg
    10501 atttaccata gaaacgttaa cacaccttta aacaagacaa tcaaaatagc ccgttgcgta
    10561 atgcgtgttc caggctaatc ccgggaaacc aattaatatc ttcagaaata cggttattac
    10621 ttgccgacca gtcggcgtgg gttaattcac gaattttcga gcgggtcagc ataggttctt
    10681 tacctgccag gcgactcaac gcggtactga tatccgcaag gcaggtgagt accggcagag
    10741 gaataccaac cattcgcacg gaaccgcaac ggacgttggc ggcaagttgc tgtatgcgtt
    10801 gccagtcata gccgccagcg acgccatcgc ataattcata ggtttgcgtc tgtacagttt
    10861 cggcgcttaa ccactgaccc actgcttgcg caaaatcggt gacgtgcagg aaagagagct
    10921 gtgtatctgg tgcaccaagt cgtggcagca ggccgcgcag catccagtca aacagcggtt
    10981 ttaactcttt atcgccggga ccatacacgg ctgtcgggcg aaaaacgccc agcgtaattt
    11041 cgtcagccat tgcagtcagc cgttgttcgg cgatgtgttt ggaatttgcg taccaggaga
    11101 gctcgggatg gcgcgccgcc agcgaagaga taaacagaaa acgttggcaa aagcagctct
    11161 cttttgctgc ttgcatcagg cgcaggctgc cgtcaacgtt acagcgggtg aaaatctctt
    11221 ctttgtgccc gcgcacttgc ccggcgcaat ggaccactgc gctggctccg gcaaccagct
    11281 tgctaagtga atgagtatct tccagcgaac cgcgcaccca ggtgagatta tcattgacgt
    11341 gagcgcgggc agtacgcgtc aatgcgcgaa cgtgaaagcc gcgggcgagc aggttatcga
    11401 taatatattt accgataaac ccggtagcgc ccgtcaccgc gacagtttga ttcatgccag
    11461 ggattcctgc acattaagac tggcagcata agccttctga taacgttttt tcgcttccgc
    11521 acgggcaggc ttgccggagg acgttcgggg aatactgtgg ggcggcaaca gatcgatagc
    11581 cgcggtcacg ccaaattcgc tttggatccg tgccgccagc gcgtggataa gctgcccgcg
    11641 acgttcttcg tcgctgatcc gacactggat ctgcaaaatg attttttcct gggcggtaac
    11701 aaaagcaatc gcatcgccag aatgaatttc cggttcttgt tccgcaatat attcaatatc
    11761 ctgcggccag atattacggc cacgaataat aatcagatct ttaatgcgtc cggtgacata
    11821 cagataaccg tccagcagat aaccgaggtc gccggtgtct aaccagcccg tcgcggcaat
    11881 ctcgtcttgc gaaacctggt cgccaaagta accgctcatc aggctggggc cggagatgca
    11941 aatatggcct accacacgtt ccgcgaccgg catacctgct tcattgcgga tttcaatgcc
    12001 atgttccggc aacgctttgc cgcagttgac gaaagtcgat acggcgcgtg tctctgcacc
    12061 cggcgcgacg gctttaccct gatattcgag gatgtcgcga tccacttcgt taaccacaac
    12121 cccggaggct tcatcagaga agctgacagc cagcgcattt tctgccagtc cgtagcacgg
    12181 catgaaagtt ttattgtcaa agttaacctg acggaaacat tcagcgaatt gatggagttg
    12241 ttctgcggag atgggttctg caccaatacc agcgacgcgc cagcaggaaa gatccagttc
    12301 agcgagatct ttttcattca cgcggcgctg gcacaattca tagccaaacg gcggcgcaac
    12361 ggaaacggtg ccgcgatttt tactgataaa tttaagccat tgcagaggac gcatggcaaa
    12421 atcctgagtg cgcaaataat ctactgaaag ctgcgtggcg acggggggca ggagaaagcc
    12481 gaccagtccc atatcatggt agaaaggcag ccaggagacg cagcggtcgc cagggcgtaa
    12541 tttaatgccg tcgtggctta tagcacgtag attagccatc acttcgcgat gggtgataat
    12601 gacgccacgg ggaaaacggg tgctgccgga ggtgtactgg aggtaggcga tatcgttcgg
    12661 aactggacgc tggagcgcaa catcggcttc cggtaatgcc ttaaaccagg cgtggcttaa
    12721 aacatgtaat tcggggttgt tatgcgtcgc ggcattgacc agtggcaacc actcatcacc
    12781 agtgataatg gctgcgggct ggcaacttgc cagtaaaccc tggagtttgg cgctccagga
    12841 atcccgctga ccgacgccca ttggaatcgc caacgggacg gcgactaagc cagcatactg
    12901 acaggcaaaa aaagcctcta caaacccgct actcgtttcg gcaatcagcg ccacgcgttc
    12961 gccttttttc aggttcagcg ataacaaccg cttcgcacca gcttcggcac gcgtttttaa
    13021 tgtctgatat tccagttgat cttcaagttg gcaacgtctg tcataaaaat tcattccggc
    13081 gctactcaga gcggcgtagt ccaaagcatc aaccagcgtt ggaaaatcgg catagcgcat
    13141 aggtagggaa tgcgtaaaga ttttattaga catatacacc acgaaaaaat tggatatca
//
LOCUS       DAZKIV010000130        13080 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZKIV010000130 DAZKIV010000000
VERSION     DAZKIV010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.130"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..142)
                     /locus_tag="ACHOT6_003636"
     CDS             complement(<1..142)
                     /locus_tag="ACHOT6_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9948792.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETH"
     gene            complement(152..703)
                     /gene="ybcL"
                     /locus_tag="ACHOT6_003637"
     CDS             complement(152..703)
                     /gene="ybcL"
                     /locus_tag="ACHOT6_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HFP9948793.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(872..1204)
                     /gene="emrE"
                     /locus_tag="ACHOT6_003638"
     CDS             complement(872..1204)
                     /gene="emrE"
                     /locus_tag="ACHOT6_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter EmrE"
                     /protein_id="HFP9948794.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            1385..1504
                     /locus_tag="ACHOT6_003639"
                     /pseudo
     CDS             1385..1504
                     /locus_tag="ACHOT6_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003844207.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(1538..1852)
                     /gene="fliE"
                     /locus_tag="ACHOT6_003640"
     CDS             complement(1538..1852)
                     /gene="fliE"
                     /locus_tag="ACHOT6_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFP9948795.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2067..3725
                     /gene="fliF"
                     /locus_tag="ACHOT6_003641"
     CDS             2067..3725
                     /gene="fliF"
                     /locus_tag="ACHOT6_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFP9948796.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            3718..4713
                     /gene="fliG"
                     /locus_tag="ACHOT6_003642"
     CDS             3718..4713
                     /gene="fliG"
                     /locus_tag="ACHOT6_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFP9948797.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            4706..5392
                     /gene="fliH"
                     /locus_tag="ACHOT6_003643"
     CDS             4706..5392
                     /gene="fliH"
                     /locus_tag="ACHOT6_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFP9948798.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPIVEPEETIIEETEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHKQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5392..6765
                     /gene="fliI"
                     /locus_tag="ACHOT6_003644"
     CDS             5392..6765
                     /gene="fliI"
                     /locus_tag="ACHOT6_003644"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFP9948799.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQHTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWKASLQGLE
                     RIFPTVS"
     gene            6784..7227
                     /gene="fliJ"
                     /locus_tag="ACHOT6_003645"
     CDS             6784..7227
                     /gene="fliJ"
                     /locus_tag="ACHOT6_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFP9948800.1"
                     /translation="MAEHSALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7224..8351
                     /gene="fliK"
                     /locus_tag="ACHOT6_003646"
     CDS             7224..8351
                     /gene="fliK"
                     /locus_tag="ACHOT6_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFP9948801.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPTEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8456..8920
                     /gene="fliL"
                     /locus_tag="ACHOT6_003647"
     CDS             8456..8920
                     /gene="fliL"
                     /locus_tag="ACHOT6_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFP9948802.1"
                     /translation="MTDYAISKKSKRSLWIQILVFITLAACASAGYSYWHSHQVAAED
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            8925..9929
                     /gene="fliM"
                     /locus_tag="ACHOT6_003648"
     CDS             8925..9929
                     /gene="fliM"
                     /locus_tag="ACHOT6_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFP9948803.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            9926..10339
                     /gene="fliN"
                     /locus_tag="ACHOT6_003649"
     CDS             9926..10339
                     /gene="fliN"
                     /locus_tag="ACHOT6_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFP9948804.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10342..10707
                     /gene="fliO"
                     /locus_tag="ACHOT6_003650"
     CDS             10342..10707
                     /gene="fliO"
                     /locus_tag="ACHOT6_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFP9948805.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            10707..11444
                     /gene="fliP"
                     /locus_tag="ACHOT6_003651"
     CDS             10707..11444
                     /gene="fliP"
                     /locus_tag="ACHOT6_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFP9948806.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            11454..11723
                     /gene="fliQ"
                     /locus_tag="ACHOT6_003652"
     CDS             11454..11723
                     /gene="fliQ"
                     /locus_tag="ACHOT6_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFP9948807.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            11732..12517
                     /gene="fliR"
                     /locus_tag="ACHOT6_003653"
     CDS             11732..12517
                     /gene="fliR"
                     /locus_tag="ACHOT6_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFP9948808.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQLAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            12807..>13080
                     /gene="rcsA"
                     /locus_tag="ACHOT6_003654"
     CDS             12807..>13080
                     /gene="rcsA"
                     /locus_tag="ACHOT6_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9948809.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHF"
BASE COUNT         3210 a         3394 c         3426 g         3050 t
ORIGIN      
        1 tatgcgtctc ccaattgata actttttttc cctgacggat acgacataaa gctggcataa
       61 gaggcttaac cctgtgaatc tcatgatgtt tatgcatccg tatttcttca atctttaggt
      121 tagtcctatc ttttgccagc atactcttat cctactttac ctcataaacg ggtattatct
      181 cagcggttgc aattttattg gcattaagca tataaccaac taacgctccg ctggaattag
      241 aatccacagg aatcttatca gtttttagag cccatatttt aaactggtaa tgatggggtt
      301 tatctccttt gggaggacat gctccaccaa atcccgcata gccaaaatca tttcggcctt
      361 ggacagcacc agtcggaagt tttgtaccat cacgtcttcc tgcatcagcg ggcaaatatg
      421 ttaccgttgc tggaatatta gcaacagtcc agtgccacca tccgctgcct gtaggcgcat
      481 ctggatcata cacagttacg gcaaagcttt tggtgccttc tggagcacca gaccaggtta
      541 atgagggcga tgtattaccc ccctcacacc caaatccaga aaatacatga gacgttgtaa
      601 gttgctctcc tgtttttatt tcattactag tgacctgaaa tgctgcggcc tgcgcagaaa
      661 atgttatgaa tgccaataca cttgaaacga taagtttttt cataaaaacc tctttgttgt
      721 gacctatcgt tattttattt gatgtgcatt gatctcatta tgcaaaaagg cgcaatgatc
      781 gcgcaaatgc aatcataatt ataaagttgc ccagaacatt agccataata tgcacaaatc
      841 agattcctgt tatctcaagg atgattattt tttaatgcgg tgcgcttcgt gacaacaagt
      901 taatgactaa cacaccggca caaatcaaca tcatgcctat gatggctggc aagtccagtc
      961 gttggccaaa aaatccccag gaaagcaaac taatcagaac gataccgact cctgaccaga
     1021 tagcataagc aatccctgta ggtatataag ccagtgtctg agctaataac caaaatgatg
     1081 cacaatagca aataattgtg ccaacagagg gccataaccg tgtaaacccc tctgaaaact
     1141 tcattaaggt tgtaccaatg acctctgcaa gtattgcgcc accaagataa atataagggt
     1201 tcatagcata ttctttcctg ttcatactgg agagaattgt actacagttt gaacacaact
     1261 caccgttttc atcctggtgt atgtgtatcc agggggctct ggcgtggcaa gagataaagg
     1321 caggaacaat aatgatcaga aggaacccca ggctaacaaa agagttcact cttcctatga
     1381 gcaaagcgaa gacaaaacat gttatcgcgt ttacggatcg tggatggcag aaaactttca
     1441 ggatcaggta tccattttaa accagaaatt gagtgagttt gccccatcca tgccccaagc
     1501 ggtgagattg gaagtaatta acaacaggtt acaaaaccta cacctgcatg gacatcactt
     1561 cctgatacgc cgccaccagc ttattacgca cctgaatccc catttgcata gaaactgagg
     1621 ctttttgcat atcggtcatc acatcgttta acgccacgcc gggttcaccg agggtgaatt
     1681 tttctgcctg cgtgcgggca gctgtttgtg tatcgctaat gcgatcgagc gcggcgtgca
     1741 gctgcccggc aaaactaatg gtcggttgcg gcagtgaatc ctgcgcacgc gcactcatcg
     1801 ccgtagcctg taactggctg ataacccctt caatcccctg tatcgctgac attttcgtct
     1861 cctggataat ttctggtagc aaagcctacc agtaagtcaa taagacaaag gcgctaaata
     1921 gaaacaaaaa aacgggttta ttggcggata gaaaaaaacg aaagcacaaa taattgcagc
     1981 gtcaattttt cgagtttgct gacccgggag tgagtcttgt tccactttgc caataacgcc
     2041 gtccataatc agccacgagg tgcgcgatga atgcgactgc agcccagaca aaatctcttg
     2101 agtggcttaa tcgcctgcgt gcgaatccga aaattccatt gattgttgcc ggttccgcgg
     2161 cagtggcggt catggtcgca ctgatcctgt gggcgaaagc ccccgactac cgcacattat
     2221 tcagcaatct ttccgatcag gatggtggcg caattgtcag ccaactgacg caaatgaata
     2281 ttccttaccg cttcagcgaa gccagcggcg ctattgaagt tccggcagat aaagttcacg
     2341 aactgcgtct gcgcctggca caacaaggtt tgcccaaagg cggcgcggtc ggtttcgaac
     2401 tactcgatca ggaaaagttt ggtatcagcc agttcagcga acaggtgaat tatcagcggg
     2461 cgctggaagg cgagctttct cgtaccatcg caactatcgg cccggtaaaa ggggcgcgcg
     2521 tacatctggc aatgccgaaa ccgtctttat tcgtccgtga acaaaaatcc ccttctgcat
     2581 cggtgacggt aaatctgtta cccggccgcg cactcgatga agggcaaatt agcgccattg
     2641 tgcatctggt ttccagcgcc gttgctggtc tgccgccggg aaacgtcacg ctggtggatc
     2701 agggcggaca tctgttaacc cagtccaata ccagcgggcg cgatcttaat gatgctcagt
     2761 tgaaatatgc cagcgatgtc gaaggccgta ttcagcggcg tattgaagca atcctgtcgc
     2821 ctattgttgg taacggtaat attcacgccc aggtcacggc gcagctggac ttcgccagta
     2881 aagaacaaac ggaagaacag tatcgcccta acggtgatga atctcatgcg gcgcttcgtt
     2941 cacgccagct taatgagagc gagcaaagcg gttccggtta tccgggcggc gtaccggggg
     3001 cgttgtcgaa tcaaccggca cctgcgaata acgcgccaat cagcacgcct ccggcaaatc
     3061 aaaataaccg ccagcagcag gcgagcacca ccagcaatag cgggccgcgt agcacacagc
     3121 ggaatgaaac cagtaactac gaagtcgatc gcaccattcg tcataccaaa atgaacgtgg
     3181 gcgatgtgca acgtctgtca gtcgcggtag tggtgaatta caaaaccttg ccagacggta
     3241 aaccattgcc tctcagcaac gaacagatga agcaaattga agatctgacc cgcgaggcga
     3301 tgggcttttc tgaaaaacgc ggtgactcgc tcaatgtcgt taactcgccg ttcaatagca
     3361 gtgacgaaag cggcggagaa ctgccgttct ggcaacagca agcgtttatc gatcagttgc
     3421 tggctgccgg tcgctggttg ctggtactgc tggtggcgtg gctgctgtgg cggaaagcgg
     3481 tacgcccgca gttaacacgt cgcgccgagg cgatgaaagc tgtacagcaa caggcgcagg
     3541 cccgtgagga agtggaagat gcggtggaag tccgcctgag caaagacgaa caactccaac
     3601 aacgacgcgc taaccaacgt ctgggggcag aagtcatgag ccagcgtatc cgtgaaatgt
     3661 ctgataacga tccgcgcgtg gtggcgctgg tcattcgcca gtggataaat aacgatcatg
     3721 agtaacctga caggcaccga taaaagcgtc atcctgctga tgaccattgg cgaagaccgg
     3781 gcggcagagg tgttcaagca cctctcccag cgcgaagtgc aaaccctgag cgctgcaatg
     3841 gcgaacgtca cgcagatctc caacaagcag ctaaccgatg tgctggcgga gtttgagcaa
     3901 gaagctgaac agtttgccgc actgaatatc aacgccaacg attatctgcg ttcggtattg
     3961 gtcaaagctc tgggtgagga acgtgccgcc agcctgctgg aagatattct cgaaactcgc
     4021 gataccgcca gcggtattga aacgctcaac tttatggagc cgcagagtgc cgccgatctg
     4081 attcgcgatg agcatccgca aattatcgcc accattctgg tgcatctgaa gcgcgcgcag
     4141 gccgccgata ttctggcgtt gttcgatgaa cgtctgcgcc acgacgtgat gttgcgtatc
     4201 gccacctttg gcggcgtgca gccagccgcg ctggcggaac tgaccgaagt actgaatggc
     4261 ttgctcgacg gtcagaatct caagcgcagc aaaatgggcg gcgtgagaac ggcagccgaa
     4321 attatcaacc tgatgaaaac tcagcaggaa gaagccgtta ttaccgccgt gcgcgaattc
     4381 gacggcgagc tggcgcaaaa aattatcgac gagatgttcc tgttcgaaaa tctggtggat
     4441 gtcgacgatc gcagcattca gcgtctgttg caggaagtgg attccgaatc gctgttgatc
     4501 gcgctgaaag gggccgagca gccactgcgc gagaaattcc tgcgcaatat gtcgcagcgt
     4561 gccgccgata ttctgcgcga cgatctcgcc aaccgtggtc cggtgcgtct gtcgcaggtg
     4621 gaaaacgaac agaaagcgat tctgctgatt gtgcgccgcc ttgccgaaac tggcgagatg
     4681 gtaattggca gcggcgagga tacctatgtc tgataatctg ccctggaaaa cctggacgcc
     4741 ggacgatctc gcgccaccac aggcagagtt tgtgccaata gttgagccgg aagaaaccat
     4801 cattgaagag accgaaccca gccttgagca gcaactggcg caactgcaaa tgcaggccca
     4861 cgagcaaggt tatcaggcgg ggattgccga aggtcgccag caaggtcata agcagggcta
     4921 tcaggaaggc ctggctcagg ggctggagca aggtctggca gaggcgaagt ctcaacaagc
     4981 gccaattcat gcccggatgc agcaactggt cagcgaattt caaactaccc ttgatgcgct
     5041 tgatagtgtg atagcgtcgc gcctgatgca gatggcgctg gaggcggcac gtcaggtcat
     5101 cggtcagacg ccaacagtgg ataactcggc actgatcaaa cagatccaac agttgttgca
     5161 gcaagaaccg ttattcagcg gcaaaccgca actacgcgtg caccccgatg atctgcaacg
     5221 tgtggatgat atgctcggcg ctaccttaag tttgcatggc tggcggttgc ggggcgatcc
     5281 cacactccat cctggcggtt gcaaagtctc cgccgatgaa ggcgatctcg acgccagtgt
     5341 cgccactcgc tggcaagaac tctgccgtct ggcagcacca ggagtggtgt aatgaccacg
     5401 cgcctaactc gctggctaac cacgctggat aactttgaag ccaaaatggc gcagttgcct
     5461 gcggtacgtc gctatgggcg attaactcgc gctaccgggc tggtgctgga agccaccgga
     5521 ttacaattgc cgctcggcgc aacctgtgtc attgagcgcc agaacggcag cgaaacgcac
     5581 gaagtagaaa gcgaagtcgt tggctttaac ggtcaacggc tgtttttaat gccgctggag
     5641 gaagtcgaag gtgtcctgcc cggcgcgcgt gtttatgcca aaaacatttc ggcagaaggg
     5701 ctgcaaagcg gcaagcagtt accgctcggt ccggcgttat taggtcgtgt tctggacggc
     5761 agcggtaaac cgctcgatgg cctcccctcc cccgatacga cggaaaccgg tgcgctgatt
     5821 accccgccat ttaacccgtt gcaacataca ccgattgaac atgtgctgga caccggcgtg
     5881 cgcccaatca atgccctgct taccgttggg cgcgggcagc gtatggggct gtttgccgga
     5941 tctggcgttg gtaaaagtgt gctgctgggg atgatggccc gttacacccg cgccgatgtc
     6001 attgtcgtgg gtttgattgg tgaacgtggg cgtgaagtaa aagattttat tgagaatatc
     6061 ctcggtgctg aagggcgtgc acgctcagta gtgattgccg ctccggcgga tgtttctcct
     6121 ctcctgcgaa tgcagggtgc cgcctatgcc acgcgcattg ccgaagattt tcgcgatcgt
     6181 ggtcagcatg tgttgctgat tatggactcc ctcacccgct acgcgatggc ccagcgtgaa
     6241 attgcgctgg cgattggcga accacccgcc actaaaggtt atcccccgtc ggtgtttgcc
     6301 aaattaccgg cactggtcga gcgtgccgga aatggcatta gcggcggcgg ctcgattacc
     6361 gcgttttata ccgtgctcac cgagggcgat gaccagcagg accccattgc cgactccgcg
     6421 cgggcaatcc tcgacggtca cattgtgctg tctcgccgac tggcggaagc cgggcactat
     6481 ccggctatcg atattgaagc gtcgatcagc cgcgcaatga cggcgttgat cagtgagcaa
     6541 cattacgcgc gagtgcgcac cttcaaacag ctgttgtcga gttttcagcg taaccgcgat
     6601 ctggttagcg tcggcgcgta tgccaaaggc agtgatccga tgctcgataa agccatcgcc
     6661 ctgtggccgc agctggaggg ctatttacaa caaggcattt ttgaacgtgc ggactggaaa
     6721 gcgtctctcc aggggctgga gcgtattttc ccgacagtgt cataacccag gagataacgg
     6781 cagatggcag aacatagcgc gctggcgacc ctgaaagatc tggcagaaaa agaggtagag
     6841 gatgccgcgc gcctgctggg tgaaatgcgt cgcgggtgtc agcaggcgga agaacagctc
     6901 aaaatgctga tcgattatca gaatgaatat cgcaataacc tcaacagcga tatgagtgcc
     6961 gggatgacca gcaaccgctg gatcaactat cagcagttta tccagacgtt ggaaaaagcc
     7021 attactcagc atcgccagca acttaatcag tggacgcaga aagttgacat tgccctgaac
     7081 agctggcgag aaaagaaaca acgtttgcag gcctggcaga cactgcagga acggcaatcc
     7141 acggcggcac tgcttgcaga aaaccgcctc gatcagaaaa agatggatga gttcgcccag
     7201 cgcgccgcca tgaggaaacc tgaatgattc gcttagcgcc cttgattacc gccgacgttg
     7261 acaccaccac attgcctggc ggcaaagcca gcgatgctgc acaagatttt ctcgcgttgt
     7321 tgagcgaagc attagcaggc gagacaacta ccgacaaagc ggccccccag ttgctggtgg
     7381 caacagataa gcccacgaca aaaggcgagc cgctggtcag cgagattctt gccgatgcgc
     7441 aacaagcgga tttactgatc cctgtggatg aaacaccgcc tgtcatcaac gacgaacaat
     7501 ccacatcaac accattaacc accgctcaaa cgatgacgtt ggctgcggtg gctggcaaca
     7561 atacggcaaa agacgaaaaa gcggatgatc tgaatgaaga cgtcaccgca agcctgagcg
     7621 ccctttttgc gatgttgccg ggttttgaca atacgcccaa agtgactgat gcgccgtcaa
     7681 ccgtgttacc gacagagaaa ccaacgctct tcacaaaact gacttctgcg caactcacaa
     7741 cagcacagcc tgatgatgcc cccggcacgc cagctcagcc attaacaccg ctggtagcag
     7801 aagcccagag taaagcggaa gtcatcagca caccgtcacc ggtgaccgct gccgccagcc
     7861 cgctaattac tccacaccag acacagccac tgcccaccgt cgccgcgcct gttttgagtg
     7921 caccgctggg ttctcacgaa tggcaacaat cattaagcca gcatatttcg ctgttcaccc
     7981 gccaggggca acaaagtgca gagttgcgtc tgcacccgca ggatttaggt gaagtgcaaa
     8041 tctccctcaa agtggatgat aaccaggcac aaatccagat ggtttcaccg catcagcatg
     8101 tacgcgccgc cctggaagca gcgctgccgg tactgcgtac gcagctggcc gaaagtggca
     8161 ttcagttagg gcaaagcaac atcagtggcg aaagctttag tggtcagcag caggccgctt
     8221 cccaacaaca gcaaagccaa cgcacagtaa accatgaacc tctggcgggg gaagaagacg
     8281 atacgcttcc ggttcccgtc tctttacaag ggcgcgtaac aggcaacagc ggcgttgata
     8341 ttttcgccta acgtcagagg tagcaccgta atccgcgtct tttccccgct ttgttgcgct
     8401 caagacgcag gataattagc cgataagcag tagcgacaca ggaagaccgc aacacatgac
     8461 tgattacgcg ataagcaaga aaagcaagcg atcgctttgg atccagattc tggtattcat
     8521 taccctcgcg gcctgtgcca gcgcaggtta cagctactgg cattcgcatc aggttgccgc
     8581 tgaagacaaa gcgcagcaac gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt
     8641 tacggtcaat ctgggcgatg cagatcgcgt actttatatc ggcataaccc tgcgcctgaa
     8701 agatgaagct acccgctcgc ggctgagtga gtatttgccg gaagtccgta gccgtttgct
     8761 gttactgttt tcgcgtcagg atgctgccgt actggcgaca gaagaaggca agaaaaacct
     8821 gattgccgag attaaaacca cgctttccac cccgcttgtt gccgggcaac cgaaacagga
     8881 tgtcaccgac gtgctgtata ccgcttttat tctgcgataa cgacatgggc gatagtattc
     8941 tttctcaagc tgaaattgat gcgctgttga atggtgacag cgaagtcaaa gacgaaccga
     9001 cagccagtgt tagcggcgaa agtgacattc gtccgtacga tccgaatacc caacgacggg
     9061 ttgtgcgcga acgtttgcag gcgctggaaa tcattaatga gcgctttgcc cgccattttc
     9121 gtatggggct gttcaacctg ctgcgtcgta gcccggatat aaccgtcggg gccatccgca
     9181 ttcagccgta ccatgaattt gcccgcaacc tgccggtgcc gaccaacctg aaccttatcc
     9241 atctgaaacc gctgcgcggc acagggctgg tggtgttttc accgagtctg gtgtttatcg
     9301 ccgtggataa cctgtttggc ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt
     9361 ttacccatac cgaacagcgc gtcatcaacc gcatgttaaa gctggcgctt gaaggctata
     9421 gcgacgcctg gaaggcgatt aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg
     9481 tgaaatttac caatatcacc acctcgccga acgacattgt ggttaacacg ccgttccatg
     9541 tggagattgg caacctgacc ggcgaattta atatctgtct gccattcagc atgattgagc
     9601 cgctacggga attgttggtt aacccgccgc tggaaaactc gcgtaatgaa gatcagaact
     9661 ggcgcgataa cctggtgcgc caggtgcagc attcacagct ggagttggtc gccaactttg
     9721 ccgatatctc gctacgcctg tcgcagattt taaaactgaa acccggcgac gtcctgccga
     9781 tagaaaaacc cgatcgcatc atcgctcatg ttgacggcgt tccggtactg accagtcagt
     9841 acggcaccct caacggtcag tatgcgttac ggatagaaca tttgattaac ccgattttaa
     9901 attctctgaa cgaggaacag cccaaatgag tgacatgaat aatccggccg atgacaacaa
     9961 cggcgcaatg gacgatctgt gggctgaagc tttgagcgaa caaaaatcaa ccagcagcaa
    10021 aagcgctgcc gacgcggtgt tccagcaatt tggcggtggt gatgtcagcg gaacgttgca
    10081 ggatatcgac ctgattatgg atattccggt caagctgacc gtcgagctgg gccgtacgcg
    10141 gatgaccatc aaagagctgt tgcgtctgac gcaagggtcc gtcgtggcgc tggacggtct
    10201 ggcgggcgaa ccactggata ttctgatcaa cggttattta attgcccagg gcgaagtggt
    10261 ggtcgttgcc gataaatatg gcgtgcggat caccgatatc attactccgt ctgagcgaat
    10321 gcgccgcctg agccgttagt gatgaataac cacgctactg tgcaatcttc cgcgccggtt
    10381 tctgctgcgc cactgctgca ggtgagcggc gcactcatcg ccattattgc cctgatcctc
    10441 gctgctgcct ggctggtaaa acggttggga tttgtcccta aacgcactgg cgttaacggt
    10501 ctgaaaatta gcgccagtgc ttcgctgggc gcgcgtgaac gggttgtggt ggtcgatgtg
    10561 gaagatgcac ggctggtgct cggcgttacc gcagggcaaa tcaatctgct gcataaactt
    10621 cccccttcag caccaacgga agagataccg cagcccgatt ttcagtcggt catgaaaaac
    10681 ttgcttaagc gtagcgggag atcctgatgc gtcgtttgtt gtctgtcgcc cctgtccttc
    10741 tctggctgat tacgcccctc gccttcgcgc aactgccggg tatcaccagc cagccgctgc
    10801 ctggcggtgg acaaagctgg tcgctcccgg tgcagacgct ggtgttcatc acctcgttga
    10861 cgtttattcc ggcaatttta ctgatgatga ccagtttcac ccgcatcatt attgtttttg
    10921 gtttattgcg taacgcgctg ggaacaccct ccgcgccacc taaccaagta ttgctggggt
    10981 tggcgctgtt tttgaccttt tttattatgt cgccggtgat cgacaaaatt tatgtcgatg
    11041 cgtaccagcc attcagcgaa gagaaaatat caatgcagga ggcgctggaa aaaggggcgc
    11101 agccgctgcg tgagtttatg ctgcgtcaga cccgtgaggc ggatttaggg ttgtttgcca
    11161 gactggcgaa tactggcccg ttgcagggac ctgaagccgt gccgatgcgc attttgctcc
    11221 cggcctacgt gaccagcgag ttgaaaaccg catttcagat aggcttcacg attttcatcc
    11281 cttttttgat tatcgacctg gtgattgcca gcgtgttgat ggcattgggg atgatgatgg
    11341 tcccccccgc caccattgct ctgcccttta aactgatgct gtttgtactg gtggatggct
    11401 ggcaattgct ggtcggttcg ctggcgcaga gcttttacag ctagagaggc aaaatgacac
    11461 ctgaatcggt catgatgatg gggactgaag cgatgaaagt cgcgctggca ctggctgccc
    11521 cgctgttgct ggtggcgctg gtcacgggcc ttatcatcag tattttgcag gccgccacgc
    11581 agatcaacga aatgacgctg tcgtttattc caaaaatcat cgccgtattt attgccatta
    11641 ttattgccgg accgtggatg ctcaatctgt tgctggatta cgtccgcacc ttgttcacta
    11701 acctgccgta tatcatcggg tagcccgtac aatgatgcag gtgacaagcg atcaatggct
    11761 ttcctggcta agtctgtact tctggccgtt actgcgcgtg ctggcgctca tttctaccgc
    11821 gccaattctg agcgaacgca gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat
    11881 cacgttcgct attgccccat cattacctgc caacgatgtt cctgtttttt cgttctttgc
    11941 tctgtggctg gccgtgcagc agatcctgat cggcattgcg cttggtttca ccatgcaact
    12001 tgcctttgcc gctgtgcgaa ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt
    12061 tgcgacgttt gtcgatccgg ccagccatct caatatgccc gttctagccc gtatcatgga
    12121 tatgctggcg ttattgctgt tcctgacatt taacggtcat ttatggttga tttcactgct
    12181 ggtcgatacc tttcacaccc tgccaattgg tggcgaaccg ttgaacagca atgcgtttct
    12241 ggcactcacc aaagcaggga gtttgatttt ccttaacggg ctaatgctgg cgttaccgct
    12301 cattactctg ctgctgacac tgaatctggc attaggttta cttaatcgta tggccccgca
    12361 attatccatt tttgttattg gatttccatt aactctgact gtcggtatct ctttaatggc
    12421 ggcattaatg ccgttaattg cacctttttg cgaacattta ttcagtgaga tgtttaattt
    12481 gctggctgat attattagtg aattgccatt aatataattc cgtaacgttt atcatgttat
    12541 cctaaggatt atccgaaaaa taatacctac gaacatcttc caggatactc ctgcggcgaa
    12601 atatttgttt taagctcact cacatatcgc aacatttact ttactttaag acaattccag
    12661 gcaaattata caacacttta cgggatagta agtccgcctg aaaaatcgcg agagtggcgc
    12721 attaggtgac ccatgttgtt ccgtttagtc atgatgaaat attcaggtaa ggggaattat
    12781 cgttacgcat tgagtgaggg tatgccatgt caacgattat tatggattta tgtagttaca
    12841 cccgactagg tttaaccggg tatctgttga gtagaggggt taaaaaaaga gaaatcaacg
    12901 acattgaaac cgttgatgac cttgccatag cttgtgattc acagcgccct tcagtggtgt
    12961 ttattaatga ggactgtttc atccatgatg cttctaacag tcagcacatc aagcacatca
    13021 ttaatcaaca tcccaatacg ttatttatcg tttttatggc aattgccaat gttcattttg
//
LOCUS       DAZKIV010000131        13020 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZKIV010000131 DAZKIV010000000
VERSION     DAZKIV010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.131"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            290..871
                     /gene="mdaB"
                     /locus_tag="ACHOT6_003655"
     CDS             290..871
                     /gene="mdaB"
                     /locus_tag="ACHOT6_003655"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFP9948810.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            902..1216
                     /locus_tag="ACHOT6_003656"
     CDS             902..1216
                     /locus_tag="ACHOT6_003656"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFP9948811.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1264..3156)
                     /gene="parE"
                     /locus_tag="ACHOT6_003657"
     CDS             complement(1264..3156)
                     /gene="parE"
                     /locus_tag="ACHOT6_003657"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFP9948812.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCTAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDKMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3185..3766)
                     /gene="yqiA"
                     /locus_tag="ACHOT6_003658"
     CDS             complement(3185..3766)
                     /gene="yqiA"
                     /locus_tag="ACHOT6_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFP9948813.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHVFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3766..4593)
                     /gene="cpdA"
                     /locus_tag="ACHOT6_003659"
     CDS             complement(3766..4593)
                     /gene="cpdA"
                     /locus_tag="ACHOT6_003659"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFP9948814.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQEQDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4618..5040)
                     /locus_tag="ACHOT6_003660"
     CDS             complement(4618..5040)
                     /locus_tag="ACHOT6_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFP9948815.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5041..5670)
                     /gene="nudF"
                     /locus_tag="ACHOT6_003661"
     CDS             complement(5041..5670)
                     /gene="nudF"
                     /locus_tag="ACHOT6_003661"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFP9948816.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5875..7356
                     /gene="tolC"
                     /locus_tag="ACHOT6_003662"
     CDS             5875..7356
                     /gene="tolC"
                     /locus_tag="ACHOT6_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFP9948817.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7504..8175
                     /locus_tag="ACHOT6_003663"
     CDS             7504..8175
                     /locus_tag="ACHOT6_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFP9948818.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8181..9341
                     /locus_tag="ACHOT6_003664"
     CDS             8181..9341
                     /locus_tag="ACHOT6_003664"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFP9948819.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9379..10167)
                     /gene="ygiD"
                     /locus_tag="ACHOT6_003665"
     CDS             complement(9379..10167)
                     /gene="ygiD"
                     /locus_tag="ACHOT6_003665"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFP9948820.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDWQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10310..11083
                     /gene="zupT"
                     /locus_tag="ACHOT6_003666"
     CDS             10310..11083
                     /gene="zupT"
                     /locus_tag="ACHOT6_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFP9948821.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11141..11311)
                     /gene="yqiD"
                     /locus_tag="ACHOT6_003667"
     CDS             complement(11141..11311)
                     /gene="yqiD"
                     /locus_tag="ACHOT6_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFP9948822.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(11573..12226)
                     /gene="ribB"
                     /locus_tag="ACHOT6_003668"
     CDS             complement(11573..12226)
                     /gene="ribB"
                     /locus_tag="ACHOT6_003668"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFP9948823.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(12330..12478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            12600..12890
                     /gene="ubiK"
                     /locus_tag="ACHOT6_003669"
     CDS             12600..12890
                     /gene="ubiK"
                     /locus_tag="ACHOT6_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFP9948824.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT         3302 a         3471 c         3164 g         3083 t
ORIGIN      
        1 taattatcaa catgacacgt tgatattttc atcttttact tattcaaagc catattttca
       61 atcccaaaaa aaaccgaacc aaacgtgtaa attattccgg tgcgagtaag aattatgagg
      121 aatggctatc agtattgtca ttttcagaaa atatttatcc tgcatcggtg agtcagagct
      181 agatcagact tttgctaaat tcgcaaaaga ctttgcacat tttgctaatt tcaccgtacc
      241 gctctgtgac gtactatagt cgacaaacgt ctcaccttga ggttaaaaaa tgagcaacat
      301 cctgattatc aacggcgcga aaaaattcgc ccactccaat ggtcaactga acgacaccct
      361 gaccgaagtc gcggatggca cactgcgcga cctcgggcat gatgtccgca tcgttcgcgc
      421 cgacagcgac tacgatgtca aagcggaagt acaaaacttt ctctgggctg atgtggtgat
      481 ctggcagatg ccaggctggt ggatgggcgc gccgtggaca gtgaaaaaat atattgatga
      541 tgtattcacc gaaggtcacg ggactctgta tgccagcgat ggtcgtaccc gcaaagatcc
      601 gtcgaaaaaa tacggttccg gcggcctggt acagggcaaa aaatatatgc tttctctaac
      661 ctggaacgca ccaatggaag ccttcaccga aaaagatcag ttcttccacg gcgttggcgt
      721 tgacggtgtg tatctgccgt tccataaagc aaaccaattc ctcggtatgg aaccgctgcc
      781 gacatttatc gctaatgacg tgataaaaat gcctgatgtt ccccgctata ctgaagaata
      841 tcgcaagcat cttgtggaaa tttttggtta actagagctc aggctttaga aggagttaac
      901 catgcttacc gtaatcgcag aaatccgtac tcgtcctggt caacatcacc gtcaggcggt
      961 attggatcag tttgctaaaa tcgttccaac cgtactgaaa gaagaaggtt gccacggcta
     1021 tgcgccaatg gtggattgcg ctgctggcgt gagtttccag tctatggcac cggattctat
     1081 cgtgatgatt gagcagtggg aaagcatcgc gcaccttgaa gcgcatctgc aaaccccgca
     1141 catgaaggcg tacagcgaag ccgtaaaagg cgacgtgctg gagatgaata tccgcatcct
     1201 gcaacctggg atttaaacct gccttgtttg cccggccatc ctgaccgggc aatgttcttt
     1261 cctttaaacc tcaatctccg ccatgtcgcc tttctcttgc aaccagttgc ggcgatcttc
     1321 cgagcgtttc ttcgccagca gcatatccat catcttgtca gtacgctgat cgtcttcatc
     1381 atcgatagtc aactgcacca gacggcgagt gttcggatca agcgtggttt cacgcaattg
     1441 catcgggttc atttccccca gacctttaaa acgctggacg ttcggcttgc ctttcttgcg
     1501 ttttaattgc tcaagtacgc cctctttctc ttcttccgtc agcgcgtaat aaacctcttt
     1561 cccgagatca atacggtaga gcggtggcag tgcgacgtaa acgtgaccgt gtttcaccaa
     1621 cgcgcggaag tgttttacga acaaagcgca gagcagcgtg gcaatgtgca gaccatcaga
     1681 gtccgcatct gcgaggatac agattttgcc ataacgaagc tggctcagat cgtcgctgtc
     1741 tggatcgata ccgatcgcta ccgaaatatc gtgcacttcc tgcgaagcca gcacttcgtc
     1801 ggaagagact tcccaggtgt taaggatctt acctttcagt ggcatgatcg cctgatattc
     1861 gcgatcgcgc gcctgcttgg cagatccgcc tgcagagtca ccttccacaa ggaacagttc
     1921 ggtacggtta agatcctgcg cggtacagtc agccagtttg ccaggcagcg ccgggccgct
     1981 ggtcagcttt ttacgcacca cttttttagc cgcacgcata cgacgctggg cgctggaaat
     2041 cgccatctcc gccagcagct ccgccgcctg aacgttctgg ttcagccaca ggatgaaggc
     2101 atctttcacc acgcctgaaa cgaatgccgt acactgacgc gaagagagac gctctttggt
     2161 ctgcccggca aactgcggat cctgcatttt caccgacaga acataggcgc agcgatccca
     2221 gatatcttcc gccgacagct tcacgccgcg cggcagaatg ttgcggtatt cacagaactc
     2281 acgcatcgcg tccaacaggc cctgacgcag accattaaca tgggtaccgc cctgcatcgt
     2341 tgggataagg ttgacgtagc tttcggtcag cagttcaccg ccttccggca gccacagtag
     2401 cgcccagtcc acagcttcag tatcaccagc gaaattaccg ataaacggtt tttccggcag
     2461 cgtcggcaga ccatttaccg cttccgccag gtaatcattc agaccgtcct gatagcacca
     2521 gcgttgttcg gtattgttga tctcatcttt aaaagtgatc tcaacgccag ggcacaatac
     2581 cgctttggct ttcagcacat gtgtcaggcg tgaaacagaa aaacgcgggc tgtcaaagaa
     2641 ggtttcatcc ggccagaagt gcacgctggt accagtattg cgtttaccgc aagtgccgac
     2701 aacctgtaaa tcctgcacct tttcgccatt ttcaaaggcg atgttataaa cctgaccatc
     2761 gcggcgcacg ttaacttcta cgcgcttcga caaggcgtta actaccgaaa tccccacgcc
     2821 atgcaggccg ccagagaact ggtaattttt gttagagaat ttaccgcccg cgtgcagacg
     2881 gcaaagaatc agttctaccg ccggtacccc ctcttccggg tgaatatcca ccggcatccc
     2941 gcgcccatcg tcaatcactt ctaacgactg gtcagcatgt aagattacat ccacgcgttt
     3001 tgcgtgaccc gccagtgctt catcgacact gttatcaata acttcttgcc ccaaatggtt
     3061 agggcgagtg gtatcggtat acatccccgg acggcggcga accggctcaa gcccggtgag
     3121 tacctcaatg gcatcagcgt tataagtttg cgtcatggtt taagttagca attcgagttg
     3181 atcgtcagag atggtgcaga ccaagaaaat cgacgatcgg gttgaaataa tcttcgaagc
     3241 ccgtgaatac gtggttgccg ccttctatga cagtctggcg gcaggaagcg tagtacgcca
     3301 ccgcctggcg gtaatccagc acttcatctc ccgtctgttg cagcagccag atcaaatccg
     3361 gcgcttccag cgggtcaatc tgcatgactt tgagatcgta aatatggcgt gactctagca
     3421 catattgctg cccggtgtag gggttctcgt tctgaccgag atagtccgtc agcagttcaa
     3481 acgggcgcac cgccgggttt accaccactg cgggcagcat aaaacattgt gacaaccagg
     3541 tggcgtaata tccccccagt gacgaaccga caatacccag cgaatcgccg ccatgttcca
     3601 ggacaatgga ttccagcagc tctgccgcgt cggaaggata cggcggcaac tgcggaatga
     3661 tcatctcaac gtcagggtga tgttccgcca gccagttttt taacaagctc gcttttgcag
     3721 agcgcggcga gctgttgaaa ccgtgtaaat aaagaagcgt agacatcagt agccttctga
     3781 agcggtatca ggttggaaac gtgtgtccgc caggcgatgc acctcggtgg tcagcgtgcc
     3841 atcagcatgt aactcgagag tacgccagcc gggcgcgatg gtgtccagcg taaagttgga
     3901 acagtgcggc ttaaactgca cacaggtcga cggcgttgcc agcaggcggc gaccgttcca
     3961 gtcgagatcc tgctcctgat gaatatgacc gcacagcaag tatttgacgt gcggaaactt
     4021 cgccagcacg gtatccagtt cgcccgcgtt acgcaggctg tgttgatcaa gccaactaca
     4081 acccgcaggt agcggatgat gatgcagcag cagcaacgta tggcgttctg gcgcatcggc
     4141 cagtttacgt tccagccact caagctgaaa ctcgctcagc tcaccgtgcg gcacgccaaa
     4201 cacctggcta tccagcaaca ggatttgcca ttgctcacca ataaacaccc gcttcgccgg
     4261 ggagataccc gcatcctgta acgcgctgta catcgcgggc tggaaatcgt ggttgccagg
     4321 cagccagacg cagggcgcac gaaaacttgc gatgccttca gcgaaatgct gataggccgc
     4381 agaggattga tcctgcgcta aatcacctgt cgcgacaatc aggtcgaatt cgtgctgatg
     4441 tggacgaatc gcctccagca ccgcctggta gctttcccag gtgtttaccc ctaacagggc
     4501 ttcgtgcttt tgtgcaaaca ggtgagtatc ggtaatttgt aaaatcctga ctctggcctc
     4561 accagccaga ggaagggtta acaggctttc caaatggtgt ccttaggttt cacgacgcta
     4621 ataaaccgga atcgccatcg ctccatgtgc taaacagtat cgcaaccagt ccgctaaaaa
     4681 ctgattaatt tgatgctttt cgtcgcgttg atgcaacttt ttattaggat aatcataccg
     4741 cgctttgaag cgaaaaatct gctggcttga acacacttca gccaccatcg cgtcatgata
     4801 cagacgcacc gtcattgacg gaaggctcca gtaactgatc gcgggcgcag tctgttctat
     4861 tgtcaccagg gtagtgtatc gggtcgattc cacaatcgtc agccgatatt gtgcgtttgc
     4921 cacctgatag cttacagttt cgccgggtgc gtcattgcgc ggtaacaaac ggcgcaattg
     4981 tgaaaagttc atctcgcaca ggcgcatcat ttcaggaaag tcaggtgtgt aacgcttcat
     5041 ttatgcccac tcatttttta acgcttgatg atgcagctgc agccattgca aagcgatgac
     5101 cgacgctgcg ttgtcgattt tcccctcttc tacccactgg tatgcctgtt cccggcttac
     5161 cacatgaacg cgaatatctt cgttttcatc agccagacca tgaataccgc ttgcggtcgt
     5221 ggcgtccact tcgcccacca taattgacga acgctcactg gtgccccccg ggcttgccag
     5281 gaaacttaac accggtttgg tccgtttgac tatcagtccc gcctcttcaa tcgcttcgcg
     5341 acgggcaaca tcttccacac tttcaccctc ttctatcatc ccggcaacca tctccagtag
     5401 ccagggggtt tcactggtgt cgtatgcggc aatccgaatc tgctcaatca gcacaacttc
     5461 atcacgcact gggtcaaagg gtagcaagac tgcggcgtga ccgcgctcaa aaatttccct
     5521 ccgtacctca tgactcattt gcccgttgaa taaacgatga cgaaatctat aaagatctaa
     5581 tgaaaaaaag ccgcgataaa gtgtttctcg tgcaataatt tctacatcgt ttttgccaaa
     5641 tgtaacgggc aggttgtctg gcttaagcat tgttaatgtc ctggcactaa tagtgaatta
     5701 aatgtgaatt tcagcgacgt ttgactgccg tttgagcagt catgtgttaa attgaggcac
     5761 attaacgccc tatggcacgt aacgccaacc ttttgcggta gcggcttctg ctagaatccg
     5821 caataatttt acagtttgat cgcgctaaat actgcttcac cacaaggaat gcaaatgaag
     5881 aaattgctcc ccattcttat cggcctgagc ctttctgggt tcagttcgtt gagccaggcc
     5941 gagaacctga tgcaagttta tcagcaagca cgccttagta acccggaatt gcgtaagtct
     6001 gccgccgatc gtgatgctgc ctttgaaaaa attaatgaag cgcgcagtcc attactgcca
     6061 cagctaggtt taggtgcaga ttacacctat agcaacggct accgcgacgc gaacggcatc
     6121 aactctaacg cgaccagtgc gtccctgcag ttaactcaat ccatttttga tatgtcgaaa
     6181 tggcgtgcgt taacgctgca ggaaaaagct gcagggattc aggacgtcac atatcagacc
     6241 gatcagcaaa ccttgatcct caacaccgcg accgcttatt tcaacgtgtt gaatgctatt
     6301 gacgttcttt cctatacaca ggcacaaaaa gaagcgatct accgtcaatt agatcaaacc
     6361 acccaacgtt ttaacgtggg cctggtagcg atcaccgacg tgcagaacgc ccgcgcgcag
     6421 tacgataccg tgctggcgaa cgaagtgacc gcacgtaata accttgataa cgcggtagag
     6481 cagctgcgcc agatcaccgg taactactat ccggaactgg cggcgctgaa tgtcgaaaac
     6541 tttaaaaccg acaaaccaca gccggttaac gcgctgctga aagaagccga aaaacgcaac
     6601 ctgtcgctgt tacaggcacg cttgagccag gacctggcgc gcgagcaaat tcgccaggcg
     6661 caggatggtc acttaccgac gctggattta acggcttcta ccgggatttc tgacacctct
     6721 tatagcggtt cgaaaacccg tggtgccgct ggtacccagt atgacgatag caatatgggc
     6781 cagaacaaag ttggcctgag cttctcgctg ccgatttatc agggcggaat ggttaactcg
     6841 caggtgaaac aggcacagta caactttgtt ggtgccagcg agcaactgga aagcgcacat
     6901 cgtagcgtcg tgcaaaccgt acgttcctcc ttcaacaaca ttaatgcttc tatcagtagc
     6961 attaacgcct acaaacaagc cgtagtttcc gctcaaagct cattagacgc gatggaagcg
     7021 ggctactcgg tcggtacgcg taccattgtt gatgtgttgg atgcgaccac cacgctgtac
     7081 aacgccaagc aagagctggc gaatgcgcgt tataactacc tgattaatca gctgaatatt
     7141 aagtcagccc tgggtacgtt gaacgagcag gatctgctgg cactgaacaa tgcgctgagc
     7201 aaaccggttt ccactaatcc ggaaaacgtt gccccgcaaa cgccggaaca gaatgctatt
     7261 gctgatggtt atgcgcctga tagcccggca cccgtcgttc agcaaacatc cgcacgcact
     7321 accaccagta acggtcataa ccctttccgt aactgatgac gacgacgggg cttcggcccc
     7381 gtctgaacgt aaggcaacgt aaagatacgg gttatctgcc gcattcttcc cccttctcgc
     7441 ttcaatttcg accagccatc ctctattctg atgggtattt accactggtc ccggaagaca
     7501 aaaatgaaac ggacaaaatc catacgccac gcatcgttcc gcaaaaactg gagcgcacgc
     7561 catctgacac cagtcgctct cgcggttgcc actgttttta tgctggctgg ctgtgaaaag
     7621 agtgatgaaa cagtgtctct ctatcaaaat gctgacgact gttcagctgc aaacccaggc
     7681 aaaagcgccg aatgtaccac cgcgtacaac aatgcgctga aagaagccga acgtactgcg
     7741 ccgaaatacg ccacccgtga agactgtgtt gctgaatttg gtgaaggtca gtgccagcaa
     7801 gcaccagccc aggctggcat ggcaccagaa aaccaggcgc aggcccagca atccagcggg
     7861 agtttctgga tgccgctgat ggccggttac atgatggggc gtctgatggg cggcggcgcg
     7921 ggatttgcac agcagccgct gttctcctcg aaaaacccag ccagtccggc ttacggtaaa
     7981 tataccgacg cgacgggtaa aaactatggc gcagcccagc caggccgcac catgaccgta
     8041 ccgaagacag ccatggcacc aaaaccggcg accaccacta ccgttacccg tggcggtttt
     8101 ggtgaatctg ttgccaaaca aagcactatg cagcgtagcg caactggcac ctcttctcgt
     8161 tcaatgggtg gctgataccg atggaaagag tcagtattac cgagcgcccg gactggcgtg
     8221 agaaagccca cgaatacggt ttcaattttc acaccatgta cggcgagccg tactggtgtg
     8281 aagatgctta ctacaagttg accctcgccc aggttgaaaa gctggaagaa gtcaccgccg
     8341 aactgcacca gatgtgcctg aaagtggtgg aaaaagtgat cgccagcgat gagctgatga
     8401 ccaaattccg cattccaaaa cacacctgga gttttgtgcg ccagtcatgg ctgacgcacc
     8461 agccatcgct ttattcgcgt cttgatctgg cgtgggatgg cactggcgaa cctaaactgc
     8521 tggaaaataa cgccgataca ccaacgtcac tatacgaggc ggcgttcttt cagtggatct
     8581 ggctggaaga tcagcttaac gccggtaact tgccggaggg cagcgaccag tttaacagtc
     8641 tgcaagaaaa gctaatcgat cgctttgtcg agttgcgtga acagtatggc ttccagttgc
     8701 tgcatctcac ctgctgtcgc gacacggtgg aagatcgcgg aaccattcag tatttgcagg
     8761 actgcgcgac ggaagctgaa atcgctaccg agttcctcta cattgacgat atcgggttag
     8821 gtgaaaaagg tcagttcacg gatttacagg atcaggtaat ttccaacctg ttcaagctgt
     8881 atccatggga atttatgttg cgtgagatgt tttccaccaa gctggaagat gctggcgtac
     8941 gctggctgga accggcgtgg aagagcatta tctccaacaa ggcacttcta ccgctactgt
     9001 gggagatgtt cccgaatcac ccgaacctgc tgcccgctta ttttgcggaa gatgatcatc
     9061 cgcaaatgga aaaatatgtg gttaaaccga tcttctcccg tgaaggcgca aacgtgtcga
     9121 tcattgagaa cggcaaaacc attgaagcag cggaaggtcc gtatggcgaa gaagggatga
     9181 ttgttcagca attccacccg ttaccgaaat tcggcgacag ctatatgttg attggtagct
     9241 ggctggtgaa cgatcaacct gccggaattg gcattcgtga agaccgtgcg ttgatcaccc
     9301 aggatatgtc tcggttttat ccacatattt ttgttgagta atcaatgatg ccggatggca
     9361 ttcgccatcc ggtaattgtt agcctatctg caccgacagc atacttaaac tgcccatttc
     9421 tataccatcg accggaatgg taattggctc ctgccaatcc cacgcaccta agacatacaa
     9481 caacggcaga tagtgctctg gcgttgggtt cgataacgca ccaccttcat ggtcgaggta
     9541 attcaccaga ggatgctgct ctaccgcccc ttgccacgtc agattcgctt tcacatactc
     9601 attaaacgac atcgcccaag gatacggtga actatcaccg tgccacttca ctgtacgcag
     9661 gttatgcacc acgttgccac tggcgaccaa cattattcct tcatctcgca gcgctgccag
     9721 tttgcgcccc atttcgaaat gccaggcggc aggtttgcta ctgtcgatac tcaactgcac
     9781 catcgggata tcggcgtcag gatacatctt aatcagcaca ccccaggaac cgtggtcaaa
     9841 gccccaggct tctttatcca gcgtcaccgg gaccggcgct aacagctcaa ccagatgctg
     9901 tgccagcaca ggcgaacccg gagcaggata atgcgtatcg tacagcgcct gcgggaagcc
     9961 accaaagtca tgaatcgtgg gcggcgtctc catcgcggtc actcctgttc cacgggtaaa
    10021 ccaatgagcc gaaaccacca caatcgcttg cgggcgtggc aatgtcatcc ccaacttctg
    10081 ccagctgcgg gtatacaaat tatcttccag cacgttcatc ggactaccgt gacctaaaaa
    10141 caatgctggc atacgtgttg aagacatgat gatatcctta actaaaggtg tcattttgat
    10201 atcctcacaa tacgcttgtt cggcggagta agaacccgga taacaatgat gatgatcatc
    10261 agttattttg acgatctgcc tgaaggtgaa gatttataag gagttgtcga tgtcagtacc
    10321 tctcattctg accatactgg cgggggcagc cacgtttatt ggcgcgtttc tcggcgttct
    10381 cgggcaaaaa ccctcgaacc gcttactggc gttttcgcta ggatttgcgg cggggatcat
    10441 gttgctcatc tcattaatgg aaatgcttcc tgccgcacta gcagctgaag gaatgtcgcc
    10501 ggtgttgggt tatggaatgt ttatcttcgg tctgcttggc tactttggcc tggaccgcat
    10561 gttgccgcat gcgcatccgc aggatttaat gcaaaaatcg gtgcagccgt tgccaaaatc
    10621 gatcaagcgc acagccattc tgcttactct cggcatcagt ctgcataact tcccggaagg
    10681 gattgccacc tttgtcacgg cgagtagcaa cctggagctg ggatttggca tcgctctggc
    10741 cgtagcattg cacaatattc ctgaaggtct ggcggttgca ggcccggttt acgcagcaac
    10801 ggggtctaaa cgcacggcga ttctgtgggc ggggatttct ggactggcag aaattcttgg
    10861 tggtgtgctg gcgtggttaa tcctcggtag catgatttcc ccggtggtca tggcggcaat
    10921 catggcggcg gttgcaggaa ttatggtggc gctctcggtt gatgaattaa tgccgctcgc
    10981 caaagagatt gaccccaata ataaccccag ctatggcgta ctatgtggaa tgtcagtgat
    11041 gggattcagt ttagtgctgc tacaaacggc gggaattggt taaaagcaac atcagaagtt
    11101 gtccagcgca tggacaactt ctgccagctt cacgaaagcc ttattctacc ttttcggctg
    11161 tctcttctct cgtactgttt aagtatttgt tccgcgcttc aagcagtgcg tctctgtcct
    11221 ggcgaaacgc ccgacaataa cgtttcaaat gcaggaaata acccaccaca accagagcaa
    11281 tcaatacaat ccagtaccag gcgataaaca tccttttacc cttaatattt aacaatacta
    11341 aacatttagc gcataaattt cacatatcct ttttcggata tattcatatg gtcgcataat
    11401 aacggaacaa ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt
    11461 ttctcataga aagagaagcg gaattaacat cttgcatagc agggcaaaat aaaaaccgaa
    11521 gccagctaca acttcggttt cttgattaag gcagtaaatt aagcagcggt tttcagctgg
    11581 ctttacgctc atgtgcctga cggtatgcca ccaggtcttc aatagtcacg agcgccatat
    11641 tgtgtttatt ggcaaactca atgcactctg gtgcacgcgc catcgtgcca tcgtcattag
    11701 tcagctcaca cagcacacca gccggtttaa agcctgccag cgtcatcaaa tcaatagtcg
    11761 cttcagtatg accgccacgc gtcagtacac cacctgcctg cgcgcgaagt gggaaaacgt
    11821 ggcccgggcg attcagatca gaaggttttg cgccatcggc aatcgctgcg cgaacggtcg
    11881 taatacgatc agcggcagaa acaccggtag tcacaccttc agccgcttca atggtcacgg
    11941 taaaaccggt gccataggcg ctggtgttat tttctaccat cattggcaga tcaagttgtt
    12001 tacggcgatc ttcagtgatg cacaggcaaa caataccgct accgtggcga atggtcagcg
    12061 ccatctgctc aacggtcatg gtttctgccg ggaagatcat atcgccttcg ttttcacggt
    12121 cttcatcatc aagcaccatt acaccgcgtc cttcacgcag cgcagccagt gcattttcaa
    12181 cacgttcgaa aggcgtacca aaagaggaaa gtagcgtctg attcatggta aaaaaacctc
    12241 actaaaatta tggttaccag aatcagggca gtcttaggag tggcggcata tagccaaaat
    12301 aacgtgagcg ggtccatgcc cgacagaatc gttactctct cccatccgga ctctaaccgt
    12361 cggccccgga attacaccgg atctgctgtc ctttgagttc gcacccaaag cgctcgcggg
    12421 ctttcaactg agttgattta ccgccggtgg ggaatttcgc cccgccctga gaataagcgg
    12481 attcactata acgctaatga ttagcggcag caacgcatag cttcacataa ttctggttta
    12541 tgacttaccc ttatcgcact acaatggcac tcaacaccta tcagtacagg gaaactacaa
    12601 tgattgaccc gaaaaaaatt gagcaaatcg ctcgccaggt tcacgaatca atgcctaaag
    12661 gaatcaggga gttcggggaa gatgtggaga aaaaaatccg ccaaacccta caagctcagc
    12721 tgacgcgcct cgaccttgta agccgtgaag agttcgacgt ccaaacgcaa gtcctgttac
    12781 ggactcgtga gaaactggcg ctgcttgaac aaagaatgag cgaactggaa aatcgcagta
    12841 ctgagattaa aaagcagcca gatccagaga ctctcccccc aacgctgtaa acatttgtct
    12901 ttaatgcact gtaaattatg aattaatcct ctaatttaca gtgctttcta ttaccttatc
    12961 gcttaattcc attcagaata tcatcacaaa aatgaatgag tgcaattgaa aatataattg
//
LOCUS       DAZKIV010000132        12929 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZKIV010000132 DAZKIV010000000
VERSION     DAZKIV010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12929
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.132"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(95..604)
                     /gene="sulA"
                     /locus_tag="ACHOT6_003670"
     CDS             complement(95..604)
                     /gene="sulA"
                     /locus_tag="ACHOT6_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFP9948825.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            823..1452
                     /gene="sxy"
                     /locus_tag="ACHOT6_003671"
     CDS             823..1452
                     /gene="sxy"
                     /locus_tag="ACHOT6_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFP9948826.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRDRLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(1415..3577)
                     /gene="yccS"
                     /locus_tag="ACHOT6_003672"
     CDS             complement(1415..3577)
                     /gene="yccS"
                     /locus_tag="ACHOT6_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFP9948827.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRHNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGVSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(3587..4033)
                     /gene="yccF"
                     /locus_tag="ACHOT6_003673"
     CDS             complement(3587..4033)
                     /gene="yccF"
                     /locus_tag="ACHOT6_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFP9948828.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            4156..6210
                     /gene="helD"
                     /locus_tag="ACHOT6_003674"
     CDS             4156..6210
                     /gene="helD"
                     /locus_tag="ACHOT6_003674"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFP9948829.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAEKAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(6242..6700)
                     /gene="mgsA"
                     /locus_tag="ACHOT6_003675"
     CDS             complement(6242..6700)
                     /gene="mgsA"
                     /locus_tag="ACHOT6_003675"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFP9948830.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(6796..7458)
                     /gene="yccT"
                     /locus_tag="ACHOT6_003676"
     CDS             complement(6796..7458)
                     /gene="yccT"
                     /locus_tag="ACHOT6_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFP9948831.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            7631..8044
                     /gene="yccU"
                     /locus_tag="ACHOT6_003677"
     CDS             7631..8044
                     /gene="yccU"
                     /locus_tag="ACHOT6_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFP9948832.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAVAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(8089..8406)
                     /gene="hspQ"
                     /locus_tag="ACHOT6_003678"
     CDS             complement(8089..8406)
                     /gene="hspQ"
                     /locus_tag="ACHOT6_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFP9948833.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(8464..9654)
                     /gene="rlmI"
                     /locus_tag="ACHOT6_003679"
     CDS             complement(8464..9654)
                     /gene="rlmI"
                     /locus_tag="ACHOT6_003679"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFP9948834.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            9749..10027
                     /gene="yccX"
                     /locus_tag="ACHOT6_003680"
     CDS             9749..10027
                     /gene="yccX"
                     /locus_tag="ACHOT6_003680"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFP9948835.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKKLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIH"
     gene            complement(10024..10353)
                     /gene="tusE"
                     /locus_tag="ACHOT6_003681"
     CDS             complement(10024..10353)
                     /gene="tusE"
                     /locus_tag="ACHOT6_003681"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFP9948836.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRNFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(10444..11103)
                     /gene="yccA"
                     /locus_tag="ACHOT6_003682"
     CDS             complement(10444..11103)
                     /gene="yccA"
                     /locus_tag="ACHOT6_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFP9948837.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(11511..12530)
                     /locus_tag="ACHOT6_003683"
     CDS             complement(11511..12530)
                     /locus_tag="ACHOT6_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9948838.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRTIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(12508..12750)
                     /locus_tag="ACHOT6_003684"
     CDS             complement(12508..12750)
                     /locus_tag="ACHOT6_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9948839.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(12818..>12929)
                     /locus_tag="ACHOT6_003685"
     CDS             complement(12818..>12929)
                     /locus_tag="ACHOT6_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9948840.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSAIWQKLIPSQADF"
BASE COUNT         3362 a         3138 c         3273 g         3156 t
ORIGIN      
        1 accgcttcag acaagcctcc gcaaggaaaa ttagtcacga ctgaaagcat tggctgggcg
       61 acaaaaaaag ttccaggatt aatcctaaat ttacttaatg atacaaatta gagtgaattt
      121 ttagcccgga aagttgtctc gtggcgtgag aggatgcgct taccggacgc ataataaacc
      181 ccatagcgtt accttcattt gccgcatcaa caagttcagc atgctcttct tcagtcaaat
      241 catctgccaa ccaaccgatc accacactgt aattgcccgt gcgtaaagcg cgaaccattg
      301 actccacagt gtggcaaggg gagagctggc taatctgcat tactttcgtt aagggtagcc
      361 cagatgcctg aacccattcc cgactcagtt tttgttgcgg tgttaaccag agttgccagc
      421 gcgattgctg accgagttgc tgtaacaatg gcaacagtag aagttgcgtc atcatgggct
      481 gatcttcgcg atagacaact tcactgataa gcccggctgt agtgttttcc gtagagacac
      541 gcgcaatttt acttgctgcg gatgagaacg acgaagaacg atgtgcatag cctgaagtgt
      601 acataatcaa tccagcccct gtgagttact gtatggatgt acagtacatc cagtgacaac
      661 aaagatcaac cctattttcg gaaagagcct cgcaaatttt gtcgttggtg acgggaaaac
      721 ataaattaat cttgcccctt aagaataagt tgcctatttt cgtagttaac ggatccgtta
      781 atgtgaatca ttcttttatg ttatgatttt aaaaggaatt ttatgaaaag cctctcctat
      841 aagcggatct ataaatcaca agaatacctg gcaacgttgg gcacaattga ataccgatca
      901 ttgtttggca gttacagcct gaccgttgac gacacggtgt ttgcgatggt ttctgatggt
      961 gagttgtatc ttcgggcttg tgagcaaagt gcacagtact gtgtaaaaca tccgcctgtc
     1021 tggctgacat ataaaaagtg tggccgatcc gttaccctca attactatcg ggttgatgaa
     1081 agtctatggc gaaatcaact gaagctggtg cgtctgtcga aatattctct cgatgcagcg
     1141 ctgaaagaga aaagcacgcg caatacccgg gatagactga aagatttgcc caatatgtct
     1201 tttcatctgg aagcgattct cggggaggtg gggattaagg atgtacgggc gttacgtata
     1261 cttggggcaa aaatgtgttg gttgcgactg cgtcagcaaa acagtctggt gacagaaaag
     1321 attctgttta tgcttgaagg tgccattatc ggcattcatg aagctgcgct cccggtggca
     1381 cgccgccagg agcttgcaga atgggctgac tctcttacgc cgaaacagga gtttcctgcg
     1441 gaacttgagt aatctggcgt tgcagacgac caatctcagg cagtaatgca atcaataatc
     1501 caacttgttg tacgaccaga ggttctttgc tgtctgcccg tggttcaagt tgctgcatcc
     1561 gctgtttcag gctagctaat gcctcattga cgcgttcttc atcagcaggt tgatgatgta
     1621 acgcgtcatc aacatagcaa actgcgtcat caagaaacgc cagaatttca ggattagtta
     1681 actgctcccg gtgagcaccg agggctgaga tatagctggt aaacgtatgg ttaaggcaca
     1741 gcaaccgaaa cgcggcttcg cgaatttgcg gggtaacgtt cggctcgctg gacatatttg
     1801 ataccaccga cgccagctca gcatcacggt tgtgtgcatc gcggcgggca atacgatacg
     1861 ccagacggtt atcacgcccc tgatggtatt gctccagtat ggcatcgaga taccgacagt
     1921 tggcctctgt ggcgcgttcg agcatgcgcg gcagattgcg aaactgccag tcaggccaga
     1981 tgtagctcac tgccgcccac gcaatggcac aaccaatcag cgtatcgatt acgcgaggta
     2041 acgctacttc aaaaccttca cccagtaagt taaaacacag tagcaccaaa agtgtgatga
     2101 acatcgttgc atgagcgtat tgcacgttac ggaaggcaaa aaagagcacg ccggtaataa
     2161 ccagcagcac cagctgccct tccagtgatg gcacaaacca cagcacagga atgccaatgg
     2221 cgatacctac cagcgtacca ataatcctta acttcaggcg gtggcgcgtg gcgttatagt
     2281 ttggctggca gacaaacaaa cttgtcagca agatccaata cccgtgatgc attccggtta
     2341 tttgaatgat ggcgtagccg aagcacaaca ccagcgacat tcttatcgca tgacggaaga
     2401 gggcggattc cggcgtgaag tgacggctaa gacgcagcca gatatcactc accccgtgcg
     2461 ggctgtcatc agcgagctca ttttcgtcat tattatgggg tagtgcctgg gcctgttctg
     2521 attcaattgt tgccagttgg gcatcaatgg cgcgtaaatt gttcagcaaa aatcccagtg
     2581 ttttgagtaa atcggcgggt gcgccgttat ggcgcatccg ctccagcgca gcatcaatat
     2641 gcgtaaaagc gcgctcaaaa tgcggatcat gttgataagg ctgacgcaac aaaatacagc
     2701 gtgacagttg ctggcacgcc tggccctgca tcgacatcag ccgctgaaaa cggaacagca
     2761 cgtcgctgtg gcgaaaatgt tcacgcaatg tttgatactg aatatgagaa gagctggcac
     2821 gctcgtgaat atcctgtgcg acaaagtaat aatgcagcgt gcgacgcgtt ccccgttgac
     2881 cacgatcgcc acgtaagcgg gtcagcagcg agagtttcgt ctgattcaat gtcgccatca
     2941 gcagaccgtt ggcgagagcc aaatcgtaca gcggtgcctg gctttcatct tcaatatcag
     3001 gatcaaacat gcgcgacttg agctcaagat aacgcgccag ttgttcatag caacgcgcca
     3061 ggttgtcctg cagcgggcgg accgggaaca gcagatgacc aataagtgtc aggacgttgt
     3121 accagacggc accggccagc agatacatcg gctgctgata ccagtgctca tacagtgatg
     3181 ttcccaacat agtgtaaatg gcgatcagca atgcaccgaa ggcaattgtt gcatagcgtt
     3241 gacccagacc gccgagcaaa atgaagccgc tggtagagag cgttaagcca atcgcaaata
     3301 gccagggcca gggaaacagc aattctactg aggccgaggc gataaaaaag cagaacagcg
     3361 taatgatgag gttacgcaaa cgtcccgcca gtcggtcatc gagatcggtc agcgccgctg
     3421 ccaccatccc cagcgttagc ggaatcgtca gttttacatc acccagccac cacggaaacg
     3481 ctgtggttcc acaaagcgca ataaaaatac gcgcgtaata cagccaggcg ctgttccagg
     3541 tatagcgttt gagcaaagga cttagcataa aggccatatt agtggattat tcaaaacgac
     3601 gacgtgcatt ggcttcacgc gcagcttgcg ctgtttctac cgataccacg cgacgaccaa
     3661 ccggccatag tgcaatagcg gcaattttaa agttcgcaat gccgacagga atgccaatga
     3721 ttgaaataca ttgtgcgatg cccgttgcaa tgtgcatcag gcataaccac cagccaaaga
     3781 aaatcagcca gaaaatattc aataccgtac cgccagtatt cagcagtaca tttttgccag
     3841 ccgggttcag ttcatcgaca tgaatagctt cattgccata aggcaccaga gacagtttag
     3901 tgatctccca gcaggatcgt gtcagcggta aggtaaaaat cagcacaata ctgaccagag
     3961 tcgccaacag ccagcccaga gtggtggcaa atccgccaag cacaaagttc agaatgttca
     4021 aaacagtacg cataaaacct cgctttactg tggttttcag taatgggcgg caattgtaac
     4081 gtttttttgg gctggagcac gttttctctg acggttacac tgataagaaa taatttcgtg
     4141 tggatctaca gagtcatgga actgaaagcg acaacgcttg gaaaacgtct ggcacagcac
     4201 ccttacgatc gggcggtgat cctcaatgcc gggattaaag tctccggcga tcgccacgaa
     4261 taccttattc ctttcaatca attactggcg attcactgta agcgcggtct ggtatggggc
     4321 gagctggaat ttgtactgcc ggacgaaaaa gtggtgcgtc tgcacggcac cgaatggggc
     4381 gagacgcagc gtttttacca tcatcttgat gctcactggc ggcggtggag tggcgagatg
     4441 agcgaaattg cgtctggtgt tttacgccag caactggatt tgattgccac gcgcactgga
     4501 gaaaataaat ggctgacgcg tgagcaaacc tctggcgttc agcaacaaat ccgccaggct
     4561 ttgtcggcgt tgccgttgcc ggttaaccga ctggaagaat tcgataactg ccgtgaggcg
     4621 tggcgtaaat gtcaggcctg gttgaaagat attgaaagcg ctcggttgca gcataaccag
     4681 gcgtataccg aagccatgct taccgagtat gcggattttt tccgccaggt cgagtcttca
     4741 ccgctgaatc cggcgcaggc ccgggcagtc gttaatggcg agcattctct gttagtgctg
     4801 gcaggtgcag gaagcggaaa aacgtcggtg ctggtggccc gtgcaggctg gttgctggcg
     4861 cgtggtgaag cgtcccctga gcaaatttta ttgctggcgt ttggtcgcaa agccgctgaa
     4921 gagatggacg agcggattcg cgaacggcta cataccgaag acattaccgc acgcacgttt
     4981 catgcgctgg cgctgcatat tattcagcag ggcagcaaaa aagttccgat agtcagcaaa
     5041 ctggaaaatg ataccgctgc ccgtcatgaa ctctttattg ctgagtggcg caagcaatgc
     5101 agcgaaaaga aagcgcaggc gaagggctgg cggcaatggc tgacggaaga aatgcagtgg
     5161 tcagtgccag aaggtaactt ctgggatgat gaaaaattac agcgtcgcct tgcctctcgc
     5221 ctcgatcgct gggtaagtct gatgcggatg cacggtggtg cacaggcaga aatgattgcc
     5281 agtgcacccg aagagattcg cgatctgttc agtaaacgta tcaagttgat ggccccgtta
     5341 ttaaaagcct ggaaaggtgc gctgaaggca gaaaaggctg tcgatttttc gggccttatt
     5401 caccaggcga ttgtgattct ggagaaaggt cgctttatca gcccgtggaa gcatattctg
     5461 gttgatgaat ttcaggatat ctcgccgcag cgcgcagcgt tgttagcggc attacgcaag
     5521 caaaacagtc agacgacgtt gttcgccgtt ggtgacgact ggcaggcgat ttaccgattc
     5581 agcggtgcgc aaatgtcgct caccaccgct ttccatgaaa actttggtga aggcgaacgt
     5641 tgtgatttag acacgactta ccgttttaac agtcgtatcg gtgaggtggc aaaccggttt
     5701 attcagcaga acccaggcca gctgaaaaag ccgctaaaca gcttaaccaa tggagacaaa
     5761 aaagccgtca cgttattgga tgagagtcaa cttgacgctt tgctggataa gctctctggt
     5821 tatgccaaac cggaagagcg cattctgatc ctggcgcgtt accatcacat gaggcctgcc
     5881 agcctggaaa aagcggcaac acgctggccg aagttgcaaa tcgactttat gaccattcat
     5941 gccagcaaag ggcaacaggc ggattacgtc atcatcgttg gcttgcagga gggaagtgat
     6001 ggttttccgg ctgcggcgcg ggagtcgatt atggaagagg cgctactgcc accggttgag
     6061 gatttcccgg acgctgaaga acggcggtta atgtacgtgg cgctgacccg ggcacgccat
     6121 cgggtatggg cactgtttaa caaagagaat ccctctccct ttgtggaaat actgaaaaat
     6181 ctggatgtgc cggtggcgag aaaaccgtaa gaaacaggtg gcgtttgcca cctgtgcaat
     6241 attacttcag acggtccgcg agataacgct gataatcggg gatcagaata tcgaccgcgt
     6301 cgttgaaatg cggcgactgg attatgaagt ctgccgttgc cacgttggtg gcgaccggaa
     6361 tgttccatac cgtcgccaga cgcagcaagg ctttcacgtc aggatcgtgc ggcacggcat
     6421 ttagtggatc ccagaagaaa atcaatacat caattttccc ttctgagatc aatgcgccaa
     6481 cctgctggtc accccccatt gggccactca acatcgcgtt gacgttcatg ccggtcgcgc
     6541 gggaaattaa gttaccggta gtgcctgttg catacagtac gtgttgttcc agtaacggtt
     6601 gatgccgttc cacccagctc atcagcattt gtttgcagtg atcgtgtgcc accagcgcaa
     6661 tatgtttccg cgcaggtaaa gtgcgagtcg tcagttccat aatgtacatc cgtagttaac
     6721 tttcctacag attactgtaa gcacttatcg ctgcaagata aagaccgaaa aagcctgcgc
     6781 acaggcacaa aaatctcagg aagatggttg tttttccgcc cactgcagga aagtatttcg
     6841 cgtttgtggg tcagccagtt taaaccaata cttcagccgt tgttctgtga gcacctgaga
     6901 ctgcggtgga atagcatcca gctcggaaac acccgaaagc agcgtactgt catctgccat
     6961 catcgtggca aattgcggta gtgaagcgcg tttcgcggat ttgttatagc gttcaacctc
     7021 gacctcataa tcaatcgttt ttgccgttga ggtaatggcg agaatatcca gctttaccgg
     7081 aatcggcgtc gcatcgccat ccaacaattc aaggcgcggc gcggcatcaa aatggttagc
     7141 ttcccgctca ttctccaggc gaggcaggcg aaaattgacc tggttgatga gctgggtatt
     7201 aaaactgacc accagcggtg gggagatata cagccgttct tcactattgg aaagatgaat
     7261 cgtcttctca acgcgaaaca ctaactgatg cggtccatta tccagttcaa tgctgtccgc
     7321 accgcgcagc agagagctgg aaaccttctt gccgtcgagt accagaagat cgacatcggt
     7381 tgaaagccgc agcgtggtgg caaaaacgga taccggcaaa cataatgcaa tcaaggtggt
     7441 cacgatgccg gttttcatag gtagctcctg tcaaaagacc gcccgaataa taatgtgtca
     7501 aaatttttct ttaatcatac ttactaacat atagacatat ttcccgcttt tgctctcatt
     7561 cattcgtatt agctgcatgg ttggcatgtc ggctttgtcg tacactttgt taaacagtct
     7621 ggaggaaata atgaaagaaa ccgatattgc aggcatttta acgtccactc acaccattgc
     7681 gctggtgggg gcaagcgaca aacccgatcg tcccagctat cgcgtgatga aatatctact
     7741 cgaccagggg tatcacgtca ttccggtttc gccaaaagtt gccggcaaaa cgctgctggg
     7801 ccagcagggg tatggcacgc tggcagacgt tccggaaaag gtcgatatgg tcgatgtttt
     7861 ccgtaattca gaagcggcgt ggggcgtggc acaggaagct gttgccattg gcgcgaaaac
     7921 cctctggatg caactggggg tgattaatga gcaagcggca gtactggcac gggatgccgg
     7981 gttgaatgtg gtgatggatc gctgcccggc tattgagatc cctcgcctgg gcctggccaa
     8041 ataaaaaatc cccggaaggc aaaaaccttc cggggattcg ttcagggatc agttacgcag
     8101 acgcggggcc tggagttgtt tgcggatggt ctgcgccagc tcatccatgg atggctgttc
     8161 aggatgttca tcctgcaatt cgctacttag ctgggcttca gccaggtagg tatgaaccgg
     8221 taggccgtta tcgtcctcca tcactacgtg ataccacgga gcggcgcgaa gctcgtcatt
     8281 taccgccagc tcatcaggcg acggttcaga gagcgaataa accgggtcga tatccacgac
     8341 cactccgaga taacctaaca gggaatggcg gacctgctgg ccgataccga atttgctggc
     8401 aatcatagtc acctcccggg aaatttttac ctttgatata agggtaaaat tccacatttc
     8461 aagttacatg acgcgacagg caaacccttt cagatatagc ccttccggat aggtagcgat
     8521 caccggatga tcggctgcct gacggaactg ctctataaat tgtacatcac ggccggcatc
     8581 aattgcggca tccgcgatga ttttctgaaa taaatcgctg gtcatcagac cggaacagga
     8641 gaaagtcagg agaataccgc cttcattcag caactgaatc gccagcatgt tgatatcttt
     8701 atagccacga cacgcgccca tcaactggct tttattctca acaaacttcg gcgggtccat
     8761 cacgataacg tcaaattttt caccgcgatc gcgataagta cgcagcaatt taaagacatc
     8821 atcacggaca aactcagcct tgctcagatc cagtttgttc agctcaacgt tctgccgtgc
     8881 aatatccagc gcttcctggg aggtatcaac gctgacaacc tggctgcaac cgcccatcag
     8941 tgccgatacg gcgaaaccac cggtatagga gaaacagttc agcacacgtt tattttcaac
     9001 gtagcggcgg gtagccaggc ggctatcacg ctggtccagg tagtagcccg ttttgtgtcc
     9061 gtgctgaata tccaccagca gtttcatgcc gtgttcttca atcggcagca gggcaggtgg
     9121 caactcgccg gtgacgaggc cctgggtcag ctccatccct tcttttttac gtaccgcaac
     9181 atcgctgcga tcgtaaatcg cacattccgg gtacagcgtt tgcagggcac taattaatgc
     9241 cgcgcgctga tattctgcgc cagcactcag cagttgcagc accagaaaat taccgaaacg
     9301 atcgatagta atacccggca ggccatcaga ttctccggcg attaaacgat agctgtcgag
     9361 gccatctttt tgcgccagcc agtcacgcca tttttgtgct tgttgcaaac ggcgggaaaa
     9421 aaaagcaatg tcgatagact cagacgggtc aaacgtccag acgcgcgccc ggatttgcga
     9481 agctggcgaa taagcgccgc gtgctaacca ttttccctga tgatcaacaa tatcgatggt
     9541 ttcaccgagg ctggctttac cttccatgcg ggcaacggcc ccggaaaaga cccacggatg
     9601 gcgacgaagt aatgattttt cgcgcccttt ggctaacact aaacgtacac tcataattta
     9661 cttttctgtc gatgcaaaga aatgggcgtc attgtccgga gttttccatg gatttgcaac
     9721 gcgtccatta aggataagga gagcagcaat gtcgaaagtc tgcataattg cctgggttta
     9781 cgggcgagtt cagggcgtag gatttcgcta caccacgcag tacgaagcga aaaaactggg
     9841 cttaaccggg tacgccaaaa atcttgacga tggcagcgtg gaagtggttg cctgcggtga
     9901 agaagggcag gtggaaaaat taatgcagtg gttaaagagt ggcggaccgc gttctgcgcg
     9961 tgtggaacgg gtgcttagcg agccgcatca tccctcgggg gaattagccg attttcgtat
    10021 ccattaaata cattttaccg gttttggcag gccagcaatt ttggtggctt gcttagccgg
    10081 acctttcgga aacagtcgat ataaatagcg gctgttacct ttttcttcgc caaatttatt
    10141 cgccatcgct tttaccagca tacgaatcgc cggagaagta ttgaattcca gatagaaatt
    10201 acgcacaaaa cgcaccactt cccagtgttc tggcgacagc gaaatccctt cgttctctgc
    10261 aatcaccaca gccagcggct cactccactg gctgctttct ttgagatagc cttcggtatc
    10321 cgtttctatc tctttacctt cgaagatcag cataattatt actaccttaa tcagactggc
    10381 ggcagtgtaa caaaaaacaa agccccgcat aaagcgaggc tatgaaagtg ttagcgggtg
    10441 agattaatcg cggctggcga agcccagaat gctcagcagg ctgacgaaga tgttgtacag
    10501 cgaaacatac aggctaaccg tggcacgaat atagttcgtt tcaccgccat gaatgatgtt
    10561 gctggtttca aacaaaatag cgccagagga gatcaaaatg aagaccgcgc tgatcgccag
    10621 atgcagagca ggcagctgca ggaagatatt agcaaccata ccaatcagca ccaccacaat
    10681 acccgccatc agcataccgc cgaggaacga catatctttg cgggtggtca gcacatatgc
    10741 agagcagcag aagaacacta acgccgttcc gcccagtgcc atagcgatta cgtcacccat
    10801 tccggcagac agataggtgt tcagaatagg tccgaggata taacccagaa aaccggtaaa
    10861 ggcgaatgcg gagataatcc cggtcggctt attcgccgtt ttataggtca ggaacatcaa
    10921 accatacata cccaccagcg tcagaatcag acccggagat ggcagcatca gcaccgtact
    10981 ggcagttgcg gtaatcgccg aaaaggccag cgtcaggctc agcagaaaat aggtattacg
    11041 cagcacctta tgggtgctaa gcagtgatgt acggtcatgt gaagaactaa caatacgatc
    11101 cattgagtca ctctctatga cagatgtaat taattaagca gcataatgat aatgcgtaag
    11161 ggcacccaga agttttaccc atctttacgc atttgatctg gaacaggttt aacagcggat
    11221 tatcaggtca ttaagcaaat ataacgccct gagaatttcg acaggcaaaa gaaaaagggg
    11281 ttagcattta gctaacccct tatcttattt ggcggaagcg cagagattcg aactctggaa
    11341 ccctttcggg tcgccggttt tcaagaccga agaaaaacaa ataaaaatca gtgcaatacg
    11401 tgaaattgct gaaaagtcaa tcaaatttaa caccaagaaa aatcaaggta ttaggaagtt
    11461 gctcatagct agtataccaa gtctttttgc cagaaatttt tctggattgt ctacttgcta
    11521 accacgagcg gcaaatccag tgttggtgtg atttttgttt tacgatcgta aatcaacacc
    11581 tgattttctg ttttatgccc gctgaaaatt tgtttgtcgc gactgctgcc ttcgtaatct
    11641 gagatcccct tggcttttat gtcgtgaaaa ttgcacccga acggaacgcc aactttttgc
    11701 tcggctgcgc gtttagcctg attccaccag ttattcagcg ttttagctat gactttcccg
    11761 cctttggttg tattgatcac atattcgcat gtacaggaag atacatttcg ggctaactgg
    11821 atcgctgtac gtaatcgcgg tgaccattcc ttgatttgtt tggtgccggt tttgttttgc
    11881 tcaatgtaga tccctttatc cataatatcc tgccatttca actcaagcac atcaccgagt
    11941 cttgccgcac agagatagga aatctccatt gcaatgcgta actgtggaat tgcttccgca
    12001 tatatcgccg catactgttc atcggtgatg taaacagtgc gggctttaag agagaatttt
    12061 ctgactcctt tgcatgggtt attcttcaca tacccacgct catatcccca cccgtatact
    12121 cgactcaggc ttgccagttc atgatttgcc tgggtcttgc tctcaagccc tcgtttatcc
    12181 atgaaaattc ttacctgctc agttttgaca ttatcagcaa gcacttttcc gaataccatc
    12241 agcaacgcct tctgatgttg tcgataatct ttttgggttc ggggggccag ttctgtaaat
    12301 gcaggggagt ccataaacat gtgccataat ttagctacgg tcattatgtt gtggagtttt
    12361 gctttttcca gttcataatt ttgccagact ttagctacgc tggttttccg cactcttcct
    12421 agccctatag ttcttgtact tccttctggt ttccacacgt aactgtaacc attcgatcta
    12481 acccgcggtg gcagtacatt atctttttta ttttttcttg gtcttcccat tattcagcgc
    12541 ctcaaaatcg ggttcagcag aaaccagttc agatgttttt ggcatcgttg tcagtccgtg
    12601 tggaatatcc ctgcgtagaa ctattggttc gtttttagga ccgattacaa atgggatgcc
    12661 gtgcagtctt aactggtgtt gctgttttgt gtatcgctcg tatttcgtga tctctttaat
    12721 ctctgctgga gatagagtta attcgtacat gtggtcacgt gcctttacag catgaccgcc
    12781 gccactgtag ctggtgggcg gcgatatggg ttgaacatta aaaatcagcc tgactcggga
    12841 tcagtttttg ccagatagct gaaacgtatt ttgcctgata acgggcgtca tcaagtgcat
    12901 tatggcgctc accttcgaat gggatagca
//
LOCUS       DAZKIV010000133        12843 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZKIV010000133 DAZKIV010000000
VERSION     DAZKIV010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.133"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..367)
                     /gene="ypeC"
                     /locus_tag="ACHOT6_003686"
     CDS             complement(41..367)
                     /gene="ypeC"
                     /locus_tag="ACHOT6_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFP9948841.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(482..1738)
                     /locus_tag="ACHOT6_003687"
     CDS             complement(482..1738)
                     /locus_tag="ACHOT6_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFP9948842.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQL
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMHQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLTALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            1942..2907
                     /gene="glk"
                     /locus_tag="ACHOT6_003688"
     CDS             1942..2907
                     /gene="glk"
                     /locus_tag="ACHOT6_003688"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFP9948843.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHNNPGLLGSGAHLRQTLGHIL"
     gene            3126..3452
                     /locus_tag="ACHOT6_003689"
     CDS             3126..3452
                     /locus_tag="ACHOT6_003689"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFP9948844.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            3474..4721
                     /locus_tag="ACHOT6_003690"
     CDS             3474..4721
                     /locus_tag="ACHOT6_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFP9948845.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            4736..5821
                     /gene="ypdF"
                     /locus_tag="ACHOT6_003691"
     CDS             4736..5821
                     /gene="ypdF"
                     /locus_tag="ACHOT6_003691"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9948846.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            5821..6858
                     /gene="ypdE"
                     /locus_tag="ACHOT6_003692"
     CDS             5821..6858
                     /gene="ypdE"
                     /locus_tag="ACHOT6_003692"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9948847.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMGRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            6883..9378
                     /gene="ptsP"
                     /locus_tag="ACHOT6_003693"
     CDS             6883..9378
                     /gene="ptsP"
                     /locus_tag="ACHOT6_003693"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFP9948848.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGSGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9381..10238)
                     /locus_tag="ACHOT6_003694"
     CDS             complement(9381..10238)
                     /locus_tag="ACHOT6_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9948849.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(10251..10985)
                     /gene="ypdB"
                     /locus_tag="ACHOT6_003695"
     CDS             complement(10251..10985)
                     /gene="ypdB"
                     /locus_tag="ACHOT6_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFP9948850.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            complement(11000..12697)
                     /gene="ypdA"
                     /locus_tag="ACHOT6_003696"
     CDS             complement(11000..12697)
                     /gene="ypdA"
                     /locus_tag="ACHOT6_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFP9948851.1"
                     /translation="MHAIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
BASE COUNT         3043 a         3301 c         3483 g         3016 t
ORIGIN      
        1 tagcgtcgca tcaggcattg tgttccattg gcgaatgtgt ttaatggcgg tgtccatgtc
       61 cgcggccttt tccgcgatga tcgtcacgat cgcgccagcc ttcacgatag ccacgctcat
      121 acgctttacg cttatcccag ccacgatgat agccattatc atgacgccac cagcggtttt
      181 tacgccactc ataattgcga tgccagtagt cacggtcgcg ccagtgacca ccgtcccagt
      241 aattaccgta atgatcgcga tcgccaattt gtaatttgat tgatggcagt agggtgattt
      301 cacctgcgtt tgctgcaagc ggggtaaatg ccatcagggc ggccgccaga aacagtgacc
      361 tgaacattgt tattctcctt cacgatcgaa gccgtcagcg gcctgttaac gcaatattac
      421 ggggaggtaa agccccgtat catcgccata actcttaaat cataaaggag agcatttttt
      481 gctaaacgtt atcgtggggt ggctgttgct tcggacgatt gaccatcatc atcggcttac
      541 ctgctaataa cagccatgcc gggagcatga cgatacagag cagcggtagc aatgtggtat
      601 tgggtacaac gactgccgcc ataaaaagac ttaaccagcc atcgcgtgtt accaccagca
      661 cgatgccgag aatagcgcag gaaacggtta ttgccgctgg aacggcggga acgtgctcat
      721 gcagcatcaa ccctaatgcc acgccgacaa acactgccgg gaagattcgc ccaccgcgaa
      781 agccactggc ggcagcaacg accagggcgg taagtttaat caccgccagc aaaaaataat
      841 cgctggtgct gaaagcctga tttgccacca tctgctgcat ctcatccagc cctttaaaca
      901 gcgaaaccgg cccaccaata actcccagaa taccgagaat aaatccgcca atacctagca
      961 cgagcaccgg atttttcatt tgatgcatca tcgcgtgcaa ccgtggtaag caccatacgg
     1021 cgaccattcc tgctgcgatg gcaatcgccg cgacaattgc accgctgaga atatcggtca
     1081 tttccaactg tccgtaatgc gcaatgggca gtgaaaaatg gggatggaaa aataatccgg
     1141 tagtaagtgc accagctgct gccgccatta atggcgcaaa aagacgatcc catagcggaa
     1201 cttcactact gccatttaag gtttgcgaaa atatcaacgc cgccgcaaca ggtgtaccaa
     1261 acagcgcgcc gatggttccg gcagaggcta aaatagtcca ctccattcgg ttgacgcgcg
     1321 gtaacagacg agcgccaatg gcaaccgcaa gggcgatatt gacggtcatg atcggatgtt
     1381 ccggccccag gctgacgccg ccagcaagac cgagaattaa tgctacgata agtcctggta
     1441 gcgcagaggg cggaactggt gcgccgatca gcggttcaca ggcggggtct ggtccggcat
     1501 gaccctggct gaaacggata accaacccca ccgcaatacc cgttagcgtt aatacaccga
     1561 tgatccagag aggtgaatcc tgggctatcc ccagagttcc cggcagtcgt tgccagagca
     1621 aattctgtaa taccgaggcg attttcatca ccacaatcag aataagactg gacgcaatcc
     1681 caattgccac ggcggggagc gataataaca acatggttct ggctcgcgga tggagcatgt
     1741 tctattcctt atgcggggtc agatacttag tttgcccagc ttgcaaaaag gcatcgctgc
     1801 aattggtgct gaaacgataa agtaaatgtg tgacccagat cgatatttac agggagcctg
     1861 cctttccggc gttgttgtta tgcccccagg tatttacagt gtgagaaaga attattttga
     1921 ctttagcgga gcagttgaag aatgacaaag tatgcattag tcggtgatgt gggcggcacc
     1981 aacgcacgtc ttgctctgtg tgatattgcc agtggtgaaa tctcgcaggc taagacctat
     2041 tcagggcttg attaccccag cctcgaagcg gtcattcgcg tttatcttga agaacataag
     2101 gtcgaggtga aagacggctg tattgccatc gcttgcccaa ttaccggtga ctgggtggca
     2161 atgaccaacc atacctgggc gttctcaatt gccgaaatga aaaagaatct cggttttagc
     2221 catctggaaa ttattaacga ttttaccgct gtatcgatgg cgatcccgat gctgaaaaaa
     2281 gagcatctga ttcagtttgg tggcgcagaa ccggtcgaag gtaaacctat tgcggtttac
     2341 ggtgccggaa cagggcttgg ggttgcgcat ctggtccatg tcgataagcg ttgggtaagc
     2401 ttgccgggcg aaggcggtca cgttgatttt gcgccgaata gtgaagaaga ggccattatc
     2461 ctcgaaatat tgcgtgcgga aattggtcat gtttcggcgg aacgcgtgct ttctggtcct
     2521 gggctggtga atttgtatcg cgcaattgtt aaagccgaca accgcctgcc agaaaatctc
     2581 aagccaaaag atattaccga acgcgcgctg gctgacagct gcaccgattg ccgccgtgcg
     2641 ttgtcgctgt tttgcgtcat tatgggccgt tttggcggca atctggcatt aacgctggga
     2701 acctttggtg gggtttatat tgctggcggt atcgtgccgc gcttccttga gttcttcaaa
     2761 gcctctggtt tccgtgccgc atttgaagat aaagggcgct ttaaagaata tgtccatgat
     2821 attccggtgt atctcatcgt ccataacaat ccgggtcttc tcggttccgg tgcacattta
     2881 cgccagacct taggtcacat tctgtaaatc cttcctgtta tatcgggagg taactctccc
     2941 gataatcttt taaatcatac agttaattca atttttcttt gtgtcccctc acaaggccga
     3001 cctgcgtcac acttccgtac agcgggatta attctccagt aaatgcatta tttgtctggt
     3061 aacggcgatt tgttttgcac gttcataatt tcactcgtca actatcactg aatgaggaat
     3121 taacgatgag taagaaactg attgccttat gtgcctgccc aatgggcctg gctcatacct
     3181 ttatggccgc tcaggcgttg gaagaagcgg cggtagaagc cggttatgaa gtgaaaattg
     3241 aaacccaggg cgcggacggt atccagaatc gcctgacggc gcaggatatc gccgaagcaa
     3301 ccatcatcat ccactccgtg gcagttaccc cggaagataa tgagcgtttc gaatcacgcg
     3361 acgtttatga aatcactttg caggacgcaa ttaaaaacgc tgcgggcatc atcaaagaaa
     3421 tcgaagagat gattgcctct gaacaacagt aatattcgac agggataatt gttatggcca
     3481 ttaaaaaacg cagtgcaacc gttgtgcctg gcgcatccgg tgcggcagcg gcagttaaga
     3541 atccgcaggc ctctaaaagc agcttctggg gtgaactccc gcagcatgtg atgtcaggga
     3601 tttcacgcat ggtgccaacc ttaattatgg gcggtgtgat cctcgctttc agccagctga
     3661 ttgcttatag ctggcttaaa attcctgctg agatcggcat catggatgct cttaatagcg
     3721 ggaaattctc cggtttcgac ctctccttac ttaaatttgc ctggctgtcg cagtccttcg
     3781 gcggcgtgtt gtttggtttt gccattccga tgtttgctgc ttttgtggcg aactctatcg
     3841 gtggcaaact ggcgttcccg gctggtttta tcggcgggtt gatgtctacc cagccgacgc
     3901 aactgctgaa cttcgatccc agcactatgc aatgggcgac ctcttcgccg gtgccgtcaa
     3961 ccttcattgg tgcgctgatt atttctatcg tcgcgggtta ccttgtgaag tggatgaacc
     4021 agaaaatcca gttaccggat ttcctgttag cgttcaaaac cacatttttg ctaccgattc
     4081 tttccgccat ttttgtcatg ctggcgatgt actacgtcat cacccccttt ggtggctgga
     4141 tcaacggcgg tatccgtact gtgctgactg ccgcaggtga gaagggcgcg ctgatgtacg
     4201 caatggggat cgccgcggcg acagcaatcg accttggtgg cccgatcaac aaagccgcag
     4261 gtttcgttgc cttcagcttt accactgacc acgtattacc ggtcaccgct cgttctatcg
     4321 ctatcgttat tcctccgatt ggtctgggtc tggcgaccat tattgaccgt cgtttaaccg
     4381 gcaaacgcct gttcaacgct caactttatc cgcagggtaa gaccgcgatg ttccttgcct
     4441 ttatggggat tagtgagggt gcgattccgt ttgcgctgga aagccccatc accgccattc
     4501 cgtcgtatat ggtcggcgcg attgtcggct caaccgccgc tgtctggctg ggcgcagtgc
     4561 aatggttccc ggaatccgct atctgggcat ggccgctggt cactaacctg ggcgtctata
     4621 tggcggggat cgcgctgggg gcagtcataa ctgcgctgat ggtggtgttc ctgcgtctga
     4681 tgatgttccg taaaggcaaa ttgttaatcg atagcctgta agaaggaaaa tccagatgac
     4741 attactcgct tcgctgcgcg actggcttaa ggcgcaacaa ctggatgcag tgcttctctc
     4801 ctcacggcag aacaaacagc cgcatctggg gatctccacc ggatcaggct atgtggttat
     4861 tagccgtgaa agtgcgcaca ttctggtaga ttcgcgctat ttcgttgagg tggaagcccg
     4921 tgcgcaaggc taccagctgc atttgcttga cgcgacgaac acgcttacca ctatcgtcaa
     4981 tcaaatcatt gccgatgagc agttgcaaac gctcggtttt gaaggccagc aggtgagttg
     5041 ggaaaccgcg caccgctgga agtctgaact caatgcgaaa ctggttagcg ccacgccgga
     5101 tgtgctgcgg caaatcaaaa cgccagagga ggtggagata atccgcctcg cctgtgggat
     5161 tgccgatcgc ggagcggagc atattcgccg ctttattcag gcggggatga gcgagcgcga
     5221 gatagccgct gaactggagt ggtttatgcg ccagcagggc gcagaaaaag cctcttttga
     5281 caccattgtc gccagcggct ggcgcggggc gctgccgcac ggcaaagcca gcgacaagat
     5341 tgttgcagcg ggcgagtttg tcactctcga ttttggtgcg ctgtatcagg gctactgctc
     5401 tgatatgacg cgcaccttgc tggtgaatgg cgaaggggtg agcgccgaat ctcacccgct
     5461 gtttgacgtc tatcagattg tcctgcaggc acagctcgcg gcaatctctg caattcgccc
     5521 cggcgtgcgc tgccagcaga ttgacgacgc cgcgcgccgg gtcattaccg aggctggatt
     5581 tggcgactat ttcggtcata acactggtca cgctatcggc attgaagtcc atgaagatcc
     5641 gcgtttttca ccgcgggaca ccacgacgct acagccaggc atgttgctga ccgtggagcc
     5701 ggggatttat ttgccagggc aagggggcgt gcgcatcgaa gatgttgtgc tggttacccc
     5761 gcaaggcgca gaagtgctct acgccatgcc gaaaacagtg ttgctcacgg gagaggcata
     5821 atggatttat cgctattaaa agcgttgagc gaggcagatg cgatcgcctc ctcggaacag
     5881 gaagtgcggc agatcctgct ggaagaagcg gatcgcctgc aaaaagaagt gcgatttgat
     5941 ggtctgggat cggtgctgat ccgcctcaat gaatcgacag gtccgaaggt gatgatctgc
     6001 gcccatatgg acgaagtggg atttatgggg cgcagcatct cccgcgaagg cgctattgac
     6061 gtgctgccgg ttggcaacgt gcgcatggct gcccgccagc tgcagccggt gcgcatcacc
     6121 acccgtgaag agtgcaaaat tccaggcctg cttgacggcg accggcaggg gaatgacgtc
     6181 agcgccatgc gcgtggacat tggtgcgcgc tcgtatgacg aagtgatgca ggcgggaatt
     6241 cgtccaggcg atcgcgtcac ctttgataca acttttcagg ttctccctca ccagcgggtg
     6301 atggggaaag cctttgatga ccgcctcggt tgctacctgc tggtgacgtt actgcgcgaa
     6361 ctacacagcg ctgaactgcc tgcggaagtg tggctggtgg ccagttccag cgaagaggtg
     6421 gggttacgcg gcgggcaaac tgccacccgc gcggtgtcgc cggatgtcgc cattgtgctt
     6481 gataccgcct gctgggcgaa aaactttgat tatggcgcgg ctaaccatcg tcagatttgt
     6541 aacgggccga tgctggtgtt aagcgacaag tcgctgattg cgccgccaaa acttaccgcg
     6601 tggatcgaaa ccgtggcggc agaaattggc gtgccgttac aggcagatat gttcagcaac
     6661 ggcggcacgg acggcggagc ggtgcactta accggcaccg gcgtgcctac agtggtgatg
     6721 gggccagcaa cccgccacgg acattgcgcc gcatcgattg ccgattgccg tgacattttg
     6781 cagatgcagc agcttttatc tgcccttatt aaacgtctta cgcgtgagac ggttgttcaa
     6841 ctgacggatt tcagatgatc tcctgattaa cccggagctg ttatgttaac gattcaattt
     6901 ctctgtcctt tgcctaacgg tctacatgct cgtccggcgt gggaacttaa agaacagtgc
     6961 agccagtggc aaagcgaaat cacctttatc aaccatcgcc agaacgcaaa ggccgatgct
     7021 aaaagctcgc tggcgttgat tggcagtggc accttattta atgacagttg cagcctgaac
     7081 attagcggca gcgatgaaga gcaggcgcgg cgcgtgctgg aagagtacat cctggtgcgc
     7141 tttatcgaca gcgacagcgt tcagccgacg caggcagaac tgacggcgca tccgctgccg
     7201 cgttcattaa gccgccttaa cccggattta ctgtacggca atgtgctggc aagcggcgtc
     7261 ggcgtgggta cgctgaccct gttacaaagc gacagcctcg acagttatcg ggcaattccc
     7321 gccagtgcgc aagattccac ccggctggag cacagcctgg caacgcttgc cgagcaactg
     7381 aaccagcaat tgcgtgagcg tgacggcgaa agcaaaacta tcctcagcgc tcatttgtcg
     7441 ctgattcagg atgatgaatt tgcaggcaat atccgtcgcc tgatgacaga acagcatcag
     7501 ggactggggg cggcgatcat cagcaatatg gagcaggttt gcgccaaact atctgcttct
     7561 gccagcgatt atctgcgcga gcgggtcagc gacattcgcg atatcagcga acagttgttg
     7621 catatcacct ggccggaact gaagccgcgc aacaatctgg tgctggaaaa accgaccatt
     7681 ctggtggctg aagatttaac cccaagccag tttttgagcc ttgatttgaa aaatcttgcg
     7741 ggcatgattc tggagaaaac cgggcgcacc tcgcatacac tgattctggc ccgtgcctcg
     7801 gcgatcccgg tactgagtgg cttgccgctg gatgcgattg cccgttatgc cgggcaacct
     7861 gcagtgcttg acgcccagtg cggcgtgctg gcgattaacc cgaatgacgc ggtgagcggt
     7921 tattatcagg tcgcacagac gctggcggat aaacgccaaa aacaacaggc gcaggccgcc
     7981 gcgcagctgg cctattcccg tgataacaag cgtattgata ttgcggcgaa tatcggcacc
     8041 gctctggaag cgccaggcgc gtttgccaac ggcgcggaag gcgttggact gttccgtact
     8101 gaaatgctct atatggatcg cgacagcgcg ccggacgagc aggagcaatt tgaagcctac
     8161 cagcaggtgc tactggcagc gggcgacaag ccgattatct tccgcacaat ggacatcggt
     8221 ggcgataaaa gcattcctta tctgaatatt ccccaggaag agaacccgtt cctcggctat
     8281 cgcgcggtac gtatttaccc ggaatttgct ggcctgttcc gcactcaact acgggcgatt
     8341 ttgcgcgccg ccagttttgg caacgcccag ttgatgatcc cgatggtcca cagtctcgat
     8401 cagatcttat gggtaaaggg cgagatccaa aaagcgatcg ttgagcttaa gcgcgatggc
     8461 ctgcgtcatg cagagacgat tacgctgggg atcatggtgg aagtgccgtc ggtgtgctac
     8521 atcatcgatc acttctgcga tgaggtcgat ttcttcagta tcggctccaa cgatatgacc
     8581 cagtatctgt atgcggtcga tcgtaataac ccgcgcgtat cgccgctgta taacccgatt
     8641 acgccatcgt tcctgcgcat gttgcagcag attgttacca ctgcgcatca gcggggcaaa
     8701 tgggtaggca tttgcggtga actgggcggt gaaagccgtt atctgccgct actgcttggg
     8761 ctgggcctgg atgagctgag tatgagtagc ccgcgtattc cggcggtgaa aagccagctt
     8821 cgtcaactgg atagcgaggc gtgtcgggaa ctggcgcgtc aggcatgtga atgccgcagt
     8881 gcgcaggaaa ttgaagcgtt actcaccgcc tttacgccgg aagaagacgt tcgcccactg
     8941 ctggcgctgg agaatatctt tgttgatcag gattttagca ataaagagca ggcgatccag
     9001 ttcctgtgcg gcaacctcgg cgttaacggg cgcactgaac atccgtttga gctggaagaa
     9061 gatgtctggc agcgggaaga gattgttacc accggcgttg gttttggcgt agcgatcccg
     9121 cacaccaaat ctcagtggat ccgtcattcc agtatcagca ttgcccggct ggcgaaaccg
     9181 attgactggc agtcagaaat gggcgaagtc gaactggtga tcatgctgac gctgggtgct
     9241 aacgaaggga tgaatcatgt gaaagtcttc tcgcagctgg cgcgtaaact ggtgaataaa
     9301 aacttccgcc agtcgttgtt tgccgcgcaa gatgcacaaa gtatcctgac gctgctggaa
     9361 acagaattaa ccttctgacg ttagccctga aaacgggcgc tgtactctcc cggcgtcaga
     9421 ccaaactgac gccggaaaac gcgacaaaaa tagtcgctat ccggaaaacc gcaacgctgt
     9481 gccacttcat gaattgacag atgatatttc tgcaaaatca tcctcgcctt cgccattcgc
     9541 acccaacgca catactcgat aaaacgcatc gttccatgct gagcaaacag ttttgacaga
     9601 tgattgggcg tgatattaaa aaactgcgcc acgctctcgc gggtgagcgg ctgggcataa
     9661 ttatcctgaa cccagttaca aatgctgtga tagagaaaat cgccgcgcgg ctgcgaattg
     9721 cccggcggca tattcaccac gttacggcaa agatgcagca aacttaacac cagtggctga
     9781 atgatattct gctccagcgg cgaacggcta agatgggtga gggcggtaag catcgcttca
     9841 ccttcaccgc gttgcaggct gggaagctgg atctggcgag caggatgcaa caacgacgtg
     9901 gtgcggctat cgtaaaacga taacccaagc caggtcggtg cgaacaccag gcttaacagc
     9961 atcaccggtt tgttgttgat cggtaaatta gccgcgcgtg ccggaataaa cagcatttct
    10021 ccttccacca ttcgctgctg ctttgcttcc agcaggttgc cgtattcgcc gcgcagcacg
    10081 atatccagac ggggaaaatc aatacataaa ctgcccaccg gtaacgaggc aggctggctg
    10141 gcaaaccaga cgcgccccag tagttgcggg ttaagcacca ggccgctgaa cagatcggcg
    10201 aaaaattgtt gatcggcggg caagcctggt gctttcatga aagttcccga ttaaagatgc
    10261 attaactggc gaaattcttt cactttgctg cggctgaccg gcacttcaaa atccagatct
    10321 ttcagtcgca gaatgtaggt gttattaaac cacggttcga tttcgcgtat tttgttcaga
    10381 ttgacacaaa atgagcgatg gcagcggaaa aaatgcgacg gcggcagttt gctgcaaaat
    10441 tcggtaatgt tcatcggcat tacgtaggat tcacgccgcg tatagacaaa ggtcattttc
    10501 tcgtgcgctt cggcgtaata gatatcgtta attggcgtga cgattattcg ctcatctttc
    10561 accagattaa tggtgtcatt ctcacgcgtt accgtcgcgg caggcgtact gctggtctgc
    10621 tgttgttgcc aggccgcttc cagtttttgc agcatcccgg taatacgtga ctcctgatac
    10681 ggtttgagaa tgtagtcaaa cgcctccagt tcaaacgctt ctaccgcatg ttctttccac
    10741 gcggtgatga acacaataaa cggtttatgg gcgaactggc tgatgttttg cgccagcaac
    10801 acgccatcca gcgacggaat attgatatcc agaaaaatgg cgtcgacgcg gttatgctgc
    10861 aaaaacttca acacgtccag accgtcgtca aaggtgccga caatctccat ctggctgtgc
    10921 tctttaatta gccagctcag ttcctgttgt gccaggaatt cgtcttcaac aatgatgact
    10981 ttcacaatat cactccggct caaagcaata acgtagcctg tgaggcgact ggggtgcgtt
    11041 ggttaggaat gtaaaacgca atttccgttc ctggttccag gcggcggata tgcagcccct
    11101 cgccatacaa taacttcacg cgatgatgaa cattcagcag gccaatttta ttgcccggca
    11161 tttcattcgc ttcgacccgc tcaatcacct ttggatcgat gccgtgcccg gtatcccgca
    11221 ccgcaatgcg tacccgattg ccgcactctg caacgctgat ggtgacaacg cctttacctt
    11281 tgcaaggctg aataccgtgg acaatggcat tctccaccaa cggctggatc agcaggctgg
    11341 gaatgcagca attcacctct tcatcaatat catagataac cgtcagcttg tcgccaaagc
    11401 gggcctgctc aatggcaata tagtctttaa tttgatacag ctcttttttg atatcgattt
    11461 gctcatcgtc ttttaattca atgttatagc gcagataacg cgacagatta aagatcagct
    11521 ggcgagcagt atccggattc agacggattg acgatgaaat agcgttcaaa gcgttaaaca
    11581 ggaaatgggg attaattttg ctttgcaggg cgcgaagctc tgccttattt gccatttcac
    11641 gtagctgctc ggcgcgtgaa acctccagtt gggtggagat gatttgcgac agaccgaccg
    11701 ccatttcctg taatgacgag gtgatctgat gcgcgtggca gtagtaaatt ttcagcgttc
    11761 cggtcacaac gcctttctcc cacaatggga tcaccagcat ggaatgaatc tctggtgtgc
    11821 ggtgggcttc atcattgttt ttaatgatga tttttccgta attcatcgcc tgacgggtag
    11881 tcgggctaat gaagtcatcg ccattctgat agttatgttc acccacgcca acataggcca
    11941 gcacatgatc ggtattggta atcgccaccg catcggcgtg aatgtcatcg cgaataattt
    12001 cacaaacttt gcgtaatgac tcgctattga catggcgaaa cagaggtagc gttttgttgg
    12061 cgatatccag cgccagcttg gcctgccgcg cggcgctggc ctctttttcg ccctcaacgc
    12121 tttgcaccag aagcacaata aagccgatac agacgctacc gaggatcatt ggaatgccga
    12181 ttttagagac gatatcgatc cccaacgcgg tagttggtgc ccagacaatt accagaatca
    12241 tggtcaacgt ctcacacaac atgccgccga gaataccgac gcgccagcgc tgtgctttgg
    12301 ggattttcag gtttatccag cctgatatac aacccgccag aatgctggtg ataaagcagg
    12361 ggatcgccgt cacgccgccg atatcaatta aataccggtg aatacccgca atcacgccgg
    12421 taatgatgcc tacccacggg ccaaacagaa tcccgccgga catcaccgcg ataatacgta
    12481 cgttcaccag cgagccttct acgggaacgc cggaccaggt gctgaacagg gcgaacagcg
    12541 agaaaatggc ggtaacggcg agcaattctt ttggggagtg cgccgacttg tgcaacagtt
    12601 cgcgaaacag gcggatacgg atgagaaaga acaggcagat aagcattaac gctgcccgat
    12661 cgaagaccgc cagcagcatg ttgaatattg cgtgcacggg tgactctgaa taaggattaa
    12721 acctctatga taggtaacct gaaggctgat gaccagcagg ccgtttttga ggaaaaacca
    12781 taaatttaaa aggggattga aagataaatg gtgtattagc aatgtatctc ttgataatgc
    12841 act
//
LOCUS       DAZKIV010000134        12812 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZKIV010000134 DAZKIV010000000
VERSION     DAZKIV010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.134"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..1717)
                     /gene="pgi"
                     /locus_tag="ACHOT6_003697"
     CDS             complement(68..1717)
                     /gene="pgi"
                     /locus_tag="ACHOT6_003697"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFP9948852.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFVAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      1954..2150
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2242..3591
                     /gene="lysC"
                     /locus_tag="ACHOT6_003698"
     CDS             2242..3591
                     /gene="lysC"
                     /locus_tag="ACHOT6_003698"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFP9948853.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3844..4116
                     /locus_tag="ACHOT6_003699"
     CDS             3844..4116
                     /locus_tag="ACHOT6_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFP9948854.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4249..5121)
                     /gene="rluF"
                     /locus_tag="ACHOT6_003700"
     CDS             complement(4249..5121)
                     /gene="rluF"
                     /locus_tag="ACHOT6_003700"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFP9948855.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRYTSPGRKKKGR"
     gene            5333..6022
                     /gene="pepE"
                     /locus_tag="ACHOT6_003701"
     CDS             5333..6022
                     /gene="pepE"
                     /locus_tag="ACHOT6_003701"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFP9948856.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(6113..7744)
                     /locus_tag="ACHOT6_003702"
     CDS             complement(6113..7744)
                     /locus_tag="ACHOT6_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFP9948857.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(7964..11647)
                     /gene="metH"
                     /locus_tag="ACHOT6_003703"
     CDS             complement(7964..11647)
                     /gene="metH"
                     /locus_tag="ACHOT6_003703"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFP9948858.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            11847..12671
                     /gene="iclR"
                     /locus_tag="ACHOT6_003704"
     CDS             11847..12671
                     /gene="iclR"
                     /locus_tag="ACHOT6_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFP9948859.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2932 a         3446 c         3334 g         3100 t
ORIGIN      
        1 agacaagggg atttatctga taaaacaatg ccgacataac gtcggcattg ttattaaggt
       61 ttttaaatta accgcgccac gctttatagc ggttaatcag accattggtc gagctatcgt
      121 ggctgctgat ttctttatca tctttcagct ctggcagaat acggtttgcc agttgtttac
      181 ccagctccac gccccactgg tcgaaggtga agatgttcag gatcacgccc tgagtaaaga
      241 ttttgtgctc atacagcgca atcaacgcac ccaggctgaa cggagtgatt tcacgcagca
      301 ggatggagtt ggtcgggcgg ttaccttcga acactttgaa cggcaccacg tagtcaagcg
      361 ttgccggatc tttaccctga tcgcgatatt cctgctcaac cacttcgcgg gatttaccaa
      421 acgccagcgc ttcagtctga gcaaagaagt tagacagcag tttctggtga tgatcagaga
      481 gcgggttatg ggtgatagcc ggagcgacga aatcgcacgg taccattttg gttccctggt
      541 ggatcagctg gtagaacgcg tgctgaccgt tagtgcctgg ttcaccccag ataatcgggc
      601 cagtctggta atccacaacg ttaccgttac ggtcaacata cttaccgttg gactccatat
      661 tgccctgctg gaagtacgcc gcgaaacggt gcatatactg gtcatacggc agaatcgcct
      721 cagtttccgc accaaagaaa ttgttgtacc agatgccaat cagcgccagc agtacaggca
      781 ggtttttctc ggcaggcgtg gtggagaaat gcttgtccat cgcgtgtgcg ccagaaagca
      841 gttcaacgaa gttatcaaag ccgatggaga gaacaatcga caggccaatc gctgaccaca
      901 aagagtaacg accgccgacc cagtcccaga actcgaacat gttggcagta tcaataccaa
      961 actcgccaac ggctttggca ttggttgaga gcgccgcaaa gtgtttcgca acgtgttttt
     1021 catcacctgc cgctttcagg aaccagtcac gcgcgctatg ggcgttggtc atggtttcct
     1081 gagtggtgaa ggttttagat gctaccagga acagcgtggt ttccgggttt acttttttca
     1141 gcacttcagc gatgtgagtc ccatcgacgt tagaaacaaa gtgcatgttc aggtgatttt
     1201 tgtacggacg cagtgcttcg gtcaccatgt acgggccgag gtcagaaccg ccgatcccga
     1261 tgttcactac gtcagtgatt gctttgccgg tataaccctt ccactcaccg gagataatcg
     1321 cttccgagaa ggttttcatc ttctccagca ccgcgttgac ttccggcatt acgtctttgc
     1381 catcaaccaa aatcggggta ttgctacggt tacgcagcgc tacgtgcagc acagcgcggt
     1441 tttcagtgcg gttgatcttc tcgccagaga acatcgactt aatcgcgccc gccagatcgc
     1501 actctttcgc cagatcttgt aatttcgcca gcgtctcttc agtgatgcgg tttttggagt
     1561 aatccaccag catctgatcg ccgaaggttg cggagaactt agaaaaacga tcgccgtctt
     1621 tagcaaaaag atcggcgatc gtaacgtctt tcatttcatc gaagtgtttc tgtagtgcct
     1681 gccaggcagc ggtctgcgtt ggattgatgt ttttcattag caatactctt ctgattttga
     1741 gaattgtgac tttggaagat tgtagcgcca gtcacagaaa aatgtgatgg ttttagtgcc
     1801 gttagcgtaa tgttgagtgt aaacccttag cgcagtgaag catttattag ctgaactact
     1861 gaccgccagg agtggatgaa aaatccgcat gaccccgtcg ttgacaaccg ccccgctcac
     1921 cctttattta taaatgtact acctgcgcta gcgcaggcca gaagaggcgc gttgcccaag
     1981 taacggtgtt ggaggagcca gtcctgtgat aacacctgag ggggtgcatc gccgaggtga
     2041 ttgaacggct ggccacgttc atcatcggct acaggggctg aatcccctgg gttgtcacca
     2101 gaagcgttcg cagtcgggcg tttcgcaagt ggtggagcac ttctgggtga aaatagtagc
     2161 gaagtatcgc tctgcgccca cccgtcttcc gctcttccct tgtgccaagg ctgaaaatgg
     2221 atcccctgac acgaggtagt tatgtctgaa attgttgtct ccaaatttgg cggtaccagc
     2281 gtagctgatt ttgacgccat gaaccgcagc gctgatattg tgctttctga tgccaacgtg
     2341 cgtttagtag tcctctcggc ttctgctggt atcactaatc tgctggtcgc tttagcggaa
     2401 gggctggaac ctggcgagcg attcgaaaaa ctcgacgcta ttcgcaacat ccagtttgcc
     2461 attctggaac gtctgcgtta cccgaacgtt atccgtgaag agattgaacg tctgctggag
     2521 aacattactg ttctggcaga agcggcggcg ctggcaacgt ctccggcgct gacagatgag
     2581 ctggtcagcc acggcgagct gatgtcgacc ctgctgtttg tcgaaatcct gcgcgaacgc
     2641 gatgttcagg cacagtggtt tgatgtacgt aaagtgatgc gtaccaacga ccgatttggt
     2701 cgtgcagagc cagatgtagc cgcgctggcg gaactggccg cgctgcagct gctcccacgc
     2761 ctcaatgaag gcttagtgat cacccaggga tttatcggta gcgaaaataa aggtcgtaca
     2821 acgacgcttg gccgtggagg cagcgattat acggcagcct tgctggcgga ggctttacac
     2881 gcatctcgtg ttgatatctg gaccgacgtc ccgggcatct acaccaccga tccacgcgta
     2941 gtttccgcag caaaacgcat tgatgaaatc gcgtttgccg aagcggcaga gatggcaact
     3001 tttggtgcaa aagtactgca tccggcaacg ttgctacccg cagtacgcag cgatatcccg
     3061 gtctttgtcg gctccagcaa agacccacgc gcaggtggta cgctggtgtg caataaaact
     3121 gaaaatccgc cgctgttccg cgcgctggcg cttcgtcgca atcagactct gcttactttg
     3181 cacagcctga atatgctgca ttctcgcggt ttcctcgcgg aagttttcgg catcctcgcg
     3241 cggcataata tttcggtaga cttaatcacc acgtcagaag tgagcgtggc attaaccctt
     3301 gataccaccg gttcaacctc cactggcgat acgttgctga cgcaatctct gctgatggag
     3361 ctttccgcac tgtgccgggt ggaggtggaa gaaggtctgg cgctggtcgc gttgattggc
     3421 aatgacctgt caaaagcctg cggcgttggc aaagaggtat tcggcgtact ggaaccgttc
     3481 aacattcgca tgatttgtta cggcgcatcc agccataacc tgtgcttcct ggtgcccggc
     3541 gaagatgccg agcaggtggt gcaaaaactg catagtaatt tgtttgagta aatactgtat
     3601 ggcctggaag ctatatttcg ggccgtattg attttcttgt cactatgctc atcaataaac
     3661 gagcctgtac tctgttaacc aacgtcttta tcggagaata attgccttta atttttttat
     3721 ctgcatctct aattaattat cgaaagagat aaatagttaa gagaaggcaa aatgaatatt
     3781 atcagttctg ctcgcaaacg aattcccgta cactgaactc tctggctcac tgagggcacc
     3841 atcatggcac tcccccgtat tacccaaaaa gagatgactg aacgcgagca gcgtgaactt
     3901 aaaacactgc tggatcgggc gcggattgcg catggtcgcg tactgaccaa ctccgaaacc
     3961 aacagcatta agaaagagta catcgataaa ttaatggtcg agcgtgaggc tgaagcgaaa
     4021 aaagcccgcc aattgaagaa aaagcaggct tataaaccgg atcctgaagc gtcatttagc
     4081 tggtcagcaa atacgtcgac gcgcggcagg cgctaatgaa caatcaacgc ctgagatttt
     4141 cactcgtctg tgtggacaca atcttcccct caccctaacc ctctccccga aggggcgagg
     4201 ggactgtccg agctcttttt ggacgttttc atcagtctga ggcgttaatc agcgtccctt
     4261 ctttttacgc cccggcgagg tataacgctt accgttggac gccgggcgac cgcctttttc
     4321 cgccgttttt tccatcttaa cgactggacg tttgatgccc gctgttttcg gtttggcctt
     4381 cgctttaggt tttgcctcgg aagaggaatt ttcgatgagc ttaaagaggt cgattaactc
     4441 atcgtcggtt aaatcgcgcc actcccccag cggaatgccg cttaagctga cgttcataat
     4501 gcgcgtacgt tccagctttt tcacttcata gccgaaatgc tcgcacatgc gccggatctg
     4561 acggttcagc ccctgcacca gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt
     4621 gcactttttc gtcaccgtcc cgaggatcgg cacccccgcc cccataccac gaataaattc
     4681 atcggtaatc ggtttatcga ccgtcaccag atactctttc tcatgatcgt tacccgcacg
     4741 gaggatctta ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt
     4801 atccaggcgg ccaatcggga acacgcgttt gctgtggtta acgaaatcga caatgttatc
     4861 gcgctcgcca tcttcggtgg tgctgacaat accgacgggc ttgttcaggg cgataagtac
     4921 caaatcttcg gcttcccgag gctcaatcaa ctgaccgttt actttcacaa cgtcgccggg
     4981 tttcacctga tcgccaatgg tggctcgctt gccattaagg aacacattgc cttgctcgat
     5041 atagcgatcc gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg
     5101 gactgatgag tcgggcagca taaattctcc tgtaaaaaac ggaatatacc gtacctttgg
     5161 gttgataaaa aatagtcatg agcgaactat ttgttatttc cattggcttg aattcgttac
     5221 ctgctcaaat cactttccag aagcaatgtg cgacatcgtg agccatgtca gtaagtggtt
     5281 aaattcgcag tggtagcatg cctcccgtga tttcttacta actggagaag taatggaact
     5341 gcttttattg agtaactcga cgctgccggg taaagcctgg ctggaacatg cgctgccgct
     5401 gattgctgaa cagttgcagg gtcgccgttc agcggtattt attcctttcg ctggcgtaac
     5461 gcagacctgg gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc
     5521 tgtcaccggt attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt
     5581 gattgtcggc ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct
     5641 ggcaccaatt actgacgtgg ttaaacgtgg tgcgctgtat attggctgga gcgcaggcgc
     5701 taaccttgct tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg
     5761 tttcgatgcg ctaaatctgt tcccgctgca aatcaacccg cacttcacca acgcgctgcc
     5821 ggaaggccat aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc
     5881 agaactgacg attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc
     5941 tacgctgggt ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggttccgct
     6001 ggaagctggt caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata
     6061 gtgcctgatg cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact
     6121 cgtcccgtcc ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca
     6181 gcgagttcag gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc
     6241 tctgcacgtt ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa
     6301 tgcgaaaacg atgtttgctg cgacgcaaac gacgagcgct ggtgacatcg ccagagaaga
     6361 acaccgacat tgccagcttt aaattactga gcaattgctc atagagcgca tccagttcct
     6421 tcaacccatc aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc
     6481 ccatgcgctc gacgatatcg gaggcctgtt caaggttgag cgacatttcg atgatctccg
     6541 cccagcggcg cgactcttcc tccgccaact cttcttttgg catccgcgcc agatacagct
     6601 taatggcggt gtagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc
     6661 gtggctcgcc gtgcatcact ttatttagcc cttccatcat ctgttccatg gcgtcgccaa
     6721 tgcgcagggt ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga
     6781 catccagatg tttgggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt
     6841 tgcaaaaccg tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt
     6901 agaagacgtg gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct
     6961 ctgccagcaa atggacaaac ggcaggataa tcaggctacc caccagctta aacagcagac
     7021 tacccagcgc gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca
     7081 gaccggaacc gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc
     7141 ctgcggcggt cagagtcgcg gtcagcagta cagcagcaag gctggagtag ctgataatgg
     7201 cgaacatcgc gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca
     7261 cctgaacgcc gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcta
     7321 gcaaaatcag cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc
     7381 gtccgaggaa aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga
     7441 aggtgagaat acgcgccatt agcgccgtcc cgacatctgc acccagcaca atgaccagag
     7501 ccggtgcgag ggctaccaga tcctgagcga caaacgaggt caccagcatg gtggtggcat
     7561 tactgctctg taccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt
     7621 cgacgctccg gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc
     7681 gaacaatatg agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta
     7741 acacgagttc aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga
     7801 ccggaaacac gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag
     7861 attattccag cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat
     7921 tgagcgccct gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc
     7981 ccagattcgg tgccagccag cgctcaactt cggtaacgct cataccttta cggcgggcat
     8041 aatcttcaac ctgatcgcgc tgaatttgtg ctacagcgta gtacttgctg tccgggtggc
     8101 tgaagtacca acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca
     8161 tgccagtgtg tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg
     8221 ggcaggccgg atagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt
     8281 cgttgctgag gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga
     8341 gatactccgc aaaggcttcc gccagacggt cggcaagcgc tttcaccatg attttgttgt
     8401 aatcatcgtg ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca
     8461 cggcaaatgc gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga
     8521 gacagtagtt agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga
     8581 tcacatgggt gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg
     8641 ggaacaggcc caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca
     8701 tgtcgttggc gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa
     8761 tgcgcggata cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat
     8821 tacgcagagt ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg
     8881 gcgtgtaagc ctgccagtca aaagcgaaat cgttatcgcg cgccgcttcc agcgtgaccg
     8941 gtggtgtgcg cggtttctta cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg
     9001 tacgagcgac aaaatcatca cgctgagtat cggaaagcag cgccgccacc acaccaacgg
     9061 tgcgcgatgc gttctgtaca tacaccgtcg ggccgctgta gttctgctca attttcaccg
     9121 ccgtgtgcgc ttttgaggtc gtcgcgccgc caatcaatag cgggatggtg aagccctgac
     9181 gctccatctc tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa
     9241 gtccaatcag atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca
     9301 taacgccgag atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt
     9361 taccgatgtc gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc
     9421 cctgctcttt gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc
     9481 gcgccgattt gaccacctgt ggcaggaaca ttttcccttc gccaaacaga tcgccgacca
     9541 cgttcatgcc gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc
     9601 gggcttcttc ggtatcctgc tcgataaact cggtaatgcc tttgaccagc gagtattcca
     9661 gacgtttatt cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg
     9721 ttttgctgcc gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcatcgcgac
     9781 gattaagaat cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg
     9841 ccagctgccc ggcattgacg atccccatat ccatgccatt gcgaatagcg tagtagagga
     9901 acactgcgtg aatggcttcg cgcaccggat cgttgccacg gaacgagaaa gaaacgttag
     9961 atacgccgcc ggaaattagc gcgtgcggca gttcgcgttt gatgtcttca cacgcgccga
    10021 taaagtcctg cgcgtagttg ttgtgctctt caatgccggt ggcaaccgcg aagatgttcg
    10081 ggtcgaagat gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc
    10141 gacggcaaat ctcgattttc cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca
    10201 ttaccaccac tgccgcaccg taacgacgca agagcttcgc gtgatggata aaggcgtcga
    10261 cgccctcttt catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt
    10321 tttcaatgac atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt
    10381 caccggcaat cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat
    10441 ccatgttgat atcgataatc tgcgcaccgt tttccacctg ctggcgggcc acatccagtg
    10501 cctcgctgta tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg
    10561 tgcgttcacc cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg
    10621 acaaacggca ggctacgggg atttccggca gtttgcgcgg cgctaatcct tctactgcac
    10681 gactcatcgc tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa
    10741 aacccgcttg cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact
    10801 caccaaaggc gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc
    10861 gtgacagctc ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa
    10921 aggtcagagc ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga
    10981 gcgtgcgccc ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca
    11041 gcgcttcaaa ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa
    11101 cggtttcaat caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat
    11161 aggccgccac cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag
    11221 aaatagacgc cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg
    11281 gcgtgcgcgc ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga
    11341 tttccgccga cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg
    11401 tgttggtttc gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca
    11461 cttccggttt gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg
    11521 caaagcgttc accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca
    11581 taccgccgtc cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc
    11641 tgctcacact tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac
    11701 tggcataaac gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt
    11761 aactcatcgg atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt
    11821 ccacgataca gaaaaaggag gctgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa
    11881 aacccgccgt tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga
    11941 aattactgga gtggattgcc gaatccaatg gcagtgtggc actcacagag ctggcgcaac
    12001 aagccgggtt acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt
    12061 tcgtgcgtca ggtcggcgaa ctgggacatt gggcaatcgg cgcacatgcc tttatggtcg
    12121 gcagcagctt tctccagagc cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatt
    12181 taatggaaga gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag
    12241 cgattattat cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg
    12301 gtaaattgcc gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag
    12361 aacaggtgac gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg
    12421 tgtctcctgt gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg
    12481 acgatgagga acatgcactg gggctacgtt gccttgcagc gtgtattttc gatgagcacc
    12541 gcgaaccgtt tgccgcaatt tctatttccg gaccgatttc acgtattacc gatgaccgcg
    12601 tgaccgagtt tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg
    12661 gaatgcgctg actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc
    12721 aatagaataa gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt
    12781 cacacacttt tattaactca gcattatttt ta
//
LOCUS       DAZKIV010000135        12676 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZKIV010000135 DAZKIV010000000
VERSION     DAZKIV010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12676
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.135"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..189)
                     /locus_tag="ACHOT6_003705"
     CDS             complement(37..189)
                     /locus_tag="ACHOT6_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9948860.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHOT6_003706"
     CDS             complement(454..1188)
                     /gene="cysH"
                     /locus_tag="ACHOT6_003706"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFP9948861.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHOT6_003707"
     CDS             complement(1262..2974)
                     /gene="cysI"
                     /locus_tag="ACHOT6_003707"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFP9948862.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHOT6_003708"
     CDS             complement(2974..4773)
                     /gene="cysJ"
                     /locus_tag="ACHOT6_003708"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFP9948863.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNFKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLEASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5092..5454
                     /gene="queD"
                     /locus_tag="ACHOT6_003709"
     CDS             5092..5454
                     /gene="queD"
                     /locus_tag="ACHOT6_003709"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFP9948864.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5532..6803
                     /locus_tag="ACHOT6_003710"
     CDS             5532..6803
                     /locus_tag="ACHOT6_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFP9948865.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSALRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSQEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDILQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6794..7054
                     /locus_tag="ACHOT6_003711"
     CDS             6794..7054
                     /locus_tag="ACHOT6_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFP9948866.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7071..7646
                     /locus_tag="ACHOT6_003712"
     CDS             7071..7646
                     /locus_tag="ACHOT6_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFP9948867.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7794..8654)
                     /locus_tag="ACHOT6_003713"
     CDS             complement(7794..8654)
                     /locus_tag="ACHOT6_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFP9948868.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8651..9430)
                     /locus_tag="ACHOT6_003714"
     CDS             complement(8651..9430)
                     /locus_tag="ACHOT6_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFP9948869.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWRDYLRQRMQP"
     gene            complement(9408..10817)
                     /locus_tag="ACHOT6_003715"
     CDS             complement(9408..10817)
                     /locus_tag="ACHOT6_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9948870.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVQMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10839..12293)
                     /locus_tag="ACHOT6_003716"
     CDS             complement(10839..12293)
                     /locus_tag="ACHOT6_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFP9948871.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12363..>12676)
                     /locus_tag="ACHOT6_003717"
     CDS             complement(12363..>12676)
                     /locus_tag="ACHOT6_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFP9948872.1"
                     /translation="GGQWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATD
                     ITLATRSNPETNQRVLDHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLV
                     R"
BASE COUNT         2922 a         3477 c         3231 g         3046 t
ORIGIN      
        1 caattcagta cgcggggttt tacccccgcg tggtggctac ttcttcgatt cgtaagcgag
       61 aactgcctta aactccatac cacgttctct gatactcaac tcacacaacg agtcgcctac
      121 catcagcgtg aatcccagta ctgtaaaaca cagcactatg attgccacaa gggcatattt
      181 tgtcagcatg tcttgcctcc ctgcaaagaa gagactaaca ttcgaattgt ttgggttcga
      241 acgctggcct caggttgata gaaatatcgc ctggggcttt tgtccatctg gaacctcgcg
      301 aatgcttaac gccagacagc ctcaagcacc cgacgccatt ctatacctga taattcttcc
      361 agcggcgttc acgtttaaaa tcaggaatac tgctcgcaaa ataaaaacgc ccccatcatt
      421 tctgacagag gcgtttaatt tatccggcaa tatttaccct tcgtgcaatc cacattccct
      481 ttttaagcca aagaaacgtg tttcttcttc cgacatgcca ggttcccatt tacgggttgt
      541 atgggtatca ccgaccgata aatatccttc atcccataat gggtgatatt tcaggccatg
      601 tttttgcagg tactgataaa tagttcggtt atcccagtcg ataatcggca gcactttaaa
      661 tacgccacgc tgaattgcca gcaccggtaa attggcgcga ctgccggatt gttcacggcg
      721 caggccagca aaccaggttt gcgcgttaag ttctttcaga gcccggttca tcggttcgac
      781 tttgttgatg tcattgtact tttcaatgcc ttcaacgccc tgctcccaca gttttccgta
      841 gcgtgcttcc tgccaggctg cgctttcggt agcacggtac actttcaggt tgagcttgag
      901 tttgtccgtt aactcgtcaa taaagcggta ggtttccggg aacagatagc cggtgtcggt
      961 gaggatcacc ggaatatccg ggtgaatctg attcaccaga tgcaggctca ccgccgcctg
     1021 aatgccaaag ctggaagaaa gtacatattc accgggcaga ttatccagcg cccaggcaac
     1081 gcggccttca gcgtccagtt tttccagttc ggcgttagtt tctgccagcg ccagaatgcg
     1141 atctaccttc ggcagttcgt tcagggcgtt tagatcgagt ttggacatag attcctcact
     1201 gtttgccttg cctgatgcga cgctcacgcg tcttatcagg cctacaagac cgggctgatg
     1261 gttaatccca caaatcgcgc gccggatcga gcaccgggcg aatgatgccc gcacgcaccg
     1321 taaagtcgcc gaagccttca cccgcttcgc gctctttcgc ccagcgccct atcagttcat
     1381 caagcgacgc caggatttcc ggctcggtga tgttttcttt atacatccgt gggatacgtg
     1441 tcccaatgcg gttgccgcca agatgcaggt tgtagcgacc cggcgcttta cccaccaggc
     1501 ccacttccgc cagcatcgcg cgaccacaac cgttcgggca gcctgttaca cgcatcacga
     1561 tatgctcatc gctgacacca tgtttcgcca ttaaattatc gatgttgtcg ataaaagacg
     1621 gcaggaaacg ctctgcttcc gccatcgcca gcgggcaagt cgggaatgac acgcaggcca
     1681 tcgagttttc acgctgcggc gtgacggcat tcattaaacc gctctctttg gcgatcttct
     1741 cgatcttcgc tttctcgctt tccggtacac cggcgatgat cagattctgg ttcgccgtaa
     1801 tgcggaaatc gcctttgtgg atcttcgcga tctccagcag gccggttttc agcggacgcc
     1861 ccggataatc aaggatgcga ccattttcga taaacagcgt caggtgccag ttatcatcaa
     1921 tgcccttaac ccagccaata cgatcgcctc gtccggtgaa ctcatatgga cggatcggtt
     1981 caaatttgat ccccgcgcga cgctccactt ccgctttaaa cgtctcaacc cccacgcgct
     2041 ccagcgtgta tttggttttg gcatttttac gatcggttcg gttaccccag tcacgctgag
     2101 ttgtcacgac ggcttcggcc accgccagcg tatgctccag cggcagatag ccaaactcgc
     2161 tcgccgtgcg ggcgtaggtt ttcttgttgc cgtgttcgat agaaagccca ccgcccacca
     2221 gcaggttgaa gcctaccagc ttgccgtttt cggcgattgc cacgaagttc atgtcgttgg
     2281 catgcagatc gatatcgttc tgcggcggga tcactaccgt ggttttgaac ttacgcggca
     2341 ggtaggtctg accaaggatc ggttcttcat cagtagtggc gactttttcc tggtcgagcc
     2401 agatctccgc atacgcgcgg gtacgaggca acagatgctc agaaatcttc ttcgcccact
     2461 cgtacgcttc cgcgtgcagc tgcgactcgt aagggttcga ggtgcagagt acgttacggt
     2521 tcatgtcgtt agctgtcgcc agcgcatcaa gaccgaccga gtgcagcatc tggtgcaccg
     2581 gtttgacgtt ctttttcaga atgccgtgga actgaaacgt ctggcggttg gtcaggcgaa
     2641 tgctgccata gatggtgttt tcaccggcaa atttgtcgat cgcctgccac tgtttagtgg
     2701 taatcacccc acccggcaga cgacagcgaa gcagcatcgc gtggcgcggc tccagcttct
     2761 gttcagcacg ttcggcgcgg atgtcgcggt catcctgctg atacatgccg tggaagcgga
     2821 tcagcaggaa gttatcgccc ttaaagccgc cggtcagacc gtcgtttaaa tcttccgcaa
     2881 tggtgccgcg caggtagttg ctttcaagct tcatgcgctc ggcgtctgtc agttttcctt
     2941 cgaccactaa aggccctgga tgtttttcgc tcattagtag acatctcgct gataacggcg
     3001 ctctacgcgc agctcactta aaaattcatc cgccgcttcg gtgtccatgc caccaaattc
     3061 ggcaatcact tccagaagtg cctgctcaac gtctttcgcc atgcgattag cgtcgccgca
     3121 gacataaatg tgggcaccat cattgatcca gcgccacagc tccgcgccct gttcgcgcag
     3181 tttgtcttgt acgtaaactt tttctttttg gtcgcgcgac caggcaagat cgatacgtgt
     3241 cagcacgcca tctttgacgt agcgctgcca ctccacctgg tacaggaagt cttccgtaaa
     3301 gtgcggatta ccaaagaaca gccagttttt acctggcgct tcgtcggcgg cgcgttgctg
     3361 cataaaggcg cggaacggcg caataccggt gcctgggcca atcataatca ccggggtttc
     3421 cgggttagcg ggcaggcgaa agttatcgtt atgttcgata aatacgcgga cttcgccctc
     3481 ttcttccacg cgatccgcga ggaagctgga ggcaccaccg gcacgggctc ggccttccac
     3541 gtcgtaacgc accacaccaa cggtgacgtg tacttcgttc tcgacttccg cctgcgagga
     3601 ggcgatggaa tacaggcgcg gggtcagcgg gcgcagcaga ttaatcagcg cttcggcatc
     3661 cagttgcgcc ggagagaaac gcaccatgtc gacaatcggc gtcgtcgcgg cgtaatgctg
     3721 taacttcgct ttatcgccca ccagcggcag cagtgtttcg ctgcgggtaa gcgtggcgta
     3781 attctcaaca atgttggcgg tgttgacggt cagttcgaag tgccactgta gcgcttcgtt
     3841 cagaggcaac gttttgccct cgacggtgac aggttcatcg cctttcagcc acagcagttc
     3901 gacaagttct ttcaccagtg ccggatcgtt ctgataccag acgcccagcg cgtcacccgg
     3961 ctggtaacgc aggcccgagt cacctaagtc aatttcgata tggcgaacgt ctttttcaga
     4021 gttacgtccg gtaattttct ggttaaccga aaggctcgcc tccagcggag cgtctttgct
     4081 gtacgggctg gagtggattt catttaccgc gccagtagcg acggattgcg aaggtgccgc
     4141 gacaggcgca cgcgatttaa gcgcatcaac cacgcgggcg cgccactcgc tggcagcagc
     4201 ctggtattca acatcggcat cgacacggtc gagcaggcgt tcaccgccca gttccgccag
     4261 cttgctgtca aaatctttcc cggactggca gaaaaattca taagagctat cgccgaggct
     4321 aaacacggca aatgcggtgt tttccagctt cggcgctttt ttggagaaca ggaacttatg
     4381 cagcgcgacg gcttcttccg gcggttcccc ttctccttgc gttgacgtca ccacgataag
     4441 cagtttttcg ctggcgattt gtttgaattt atagtcaccc gcgttcacca gcttaaagtt
     4501 cagttttgct gctaataaat catcgcgtaa tgcttcagca acccggcgcg cattgccggt
     4561 ttgcgaggcg gagataatag ttatacccgg catttctgcg gctggcgctg gcgtcgctgc
     4621 aagcgcagca ggctgctgat tgagtacgcc ccagaaatag ccagaaaccc aggcaagctg
     4681 ggtaggcgtt aaatcggtcg tggccgcctg aaggcgtgcc agttgctccg ggttcaacgg
     4741 aagcaacgcg gaaggtggga cctgtgtcgt catgcgtcgt tatgttccag taagcaaagc
     4801 tgtttctgcg ccctgtcagc gcccataaaa cagaagagaa ggtaaggtta acggggtgaa
     4861 cggtgtggat taaagacggg atagcgataa ctaataacca aatcgactaa cctgttttag
     4921 caatagtctt taacaacaaa atagattaac caacctaatg aaaaacaaat gaatttagcc
     4981 aatcattaag ataaatcagc gattttgcgc aacaaagtcg ttttagataa tgcgaaaaaa
     5041 cagcctttcc ggtactctac ggcggtttta tcgtcctgta gagaaattat gatgtccacc
     5101 acgttattta aagatttcac cttcgaagcc gctcaccgct taccacacgt cccggaaggg
     5161 cataaatgtg gtcgcctgca cgggcattcc tttatggtgc gactggaaat taccggggaa
     5221 gtcgatccgc atacgggctg gattatcgat ttcgctgaac taaaagcggc gtttaagcca
     5281 acttatgagc gcctcgatca ccattatctc aatgatattc caggtctgga aaacccaacc
     5341 agcgaagttt tagcaaaatg gatttgggat caggttaaac ccgttgtgcc gctgttaagt
     5401 gcggtgatgg taaaagaaac ctgcaccgct ggttgtatct atcgcggcga atgataagag
     5461 tgtgtcggcg gtcaatttcc cttaagtaac gctatgttag ggtgttgtgt tctggatatc
     5521 tggggcatga catggaagac gactgcgaca ttattattat tggtgccggt attgcaggca
     5581 ccgcttgcgc gttacgctgc gcgcgagcgg gtttatccgt tttgttactg gaacgcgctg
     5641 aaatccccgg cagcaaaaat ctttccggcg ggcggttata tacccatgca ctcgcggaac
     5701 tcctcccgca atttcatctg accgcgcctc ttgaacgacg catcactcac gaaagccttt
     5761 ccctgttaac gccggatggc gcaacgacgt tttccagctt acagcccggc ggtgaatcct
     5821 ggagtgcatt acgtgcacgg ttcgatccgt ggctggttgc cgaagccgaa gaagaaggtg
     5881 tcgaatgcat ccccggagcg acggtggatg cactgtatga agaaaacggc agggtgtgtg
     5941 gtgtcatttg tggtgacgat attctccgtg cccgttatgt ggtgctggca gaaggtgcca
     6001 acagcgtcct ggctgagcgt cacgggttag tgactcgtcc tgctggtgaa gcgatggcgt
     6061 tggggatcaa agaagtgctg tcgcaggaac catccgctat tgaagaacgt tttcatctgg
     6121 agaataacga aggcgcagcg ttgctgttca gcggcaggat ctgtgatgac ttacccggcg
     6181 gcgcatttct ttatactaat caacaaacgc tctcgttagg gattgtttgc ccgctctctt
     6241 cccttacgca aagtcgtgtt ccggcaagcg agctgctgac tcgctttaaa gcgcatccgg
     6301 cagtgcgccc gcttatcaaa aacacggaat cactggagta tggtgcgcat ctggtgccag
     6361 aaggtggctt gcacagtatg ccggtgcaat acgccggtaa cggctggctg ctggtgggcg
     6421 atgcgttgcg cagttgcgtc aataccggaa tttccgtgcg cggcatggat atggcgctga
     6481 ctggcgcgca ggcggcggca caaacgctga taagcgcctg ccagcaccgc gagccgcaaa
     6541 atctgtttgc gctttatcat cacaacgtcg agcgcagcct gctatgggat attctacagc
     6601 gttatcagca tgttccggcg cttttgcaac gccctggctg gtatcgggcg tggcctgcgt
     6661 taatgcagga tatttcccgc gatttatggg atcagggtga taaacctgtt ccaccgctgc
     6721 gccagttatt ctggcgtcat ttacgtcgtc atggcctgtg gcatctggcg ggcgatgtta
     6781 tcaggagtct gcgatgtctg tagcccataa tctctggcgc gttgctgatg cgccgcacat
     6841 tgttccggct gactccgttg agcgtcagat ggtacaacgg ttgattaacg cctgtccggc
     6901 aggtcttttt tcgctcacac cggaaggtga cttacgcgtt gattatcacg gctgcctgga
     6961 gtgtggcacc tgccgtttgc tgtgcgacga atccacacta caacagtggc gctatccgcc
     7021 ttccggattc ggcatcacct accgctttgg ataaataata aggataattt atgcccctct
     7081 tacacctgct ccgccagaat ccggtgattg ctgccgttaa agataatgcc agcctgcaac
     7141 tggctatcga ttctgaatgc cagtttattt ccgtgttgta cggcaatatc tgcaccatca
     7201 gtaatatcgt taaaaagatt aagaacgccg ggaaatatgc ttttattcat gttgatttgc
     7261 tggaaggcgc gtcaaataaa gaagtcgtta ttcagttttt gaaactggtg accgaggcgg
     7321 acggcattat cagtaccaaa gcctcaatgt tgaaagcggc aagagcggaa ggttttttct
     7381 gtattcatcg cctgtttatt gttgattcaa tttcgtttca caacattgat aagcaagttg
     7441 cgcaatcgaa tccggattgt attgagatcc tgccaggctg tatgcccaaa gtgctgggct
     7501 gggtgacaga gaaaatccgc caaccgctga ttgccggtgg gctggtgtgc gatgaagaag
     7561 atgcgcgtaa tgcgattaac gcgggtgtcg tggcgctttc caccacgaat accggggtct
     7621 ggacgttagc gaaaaaatta ctttgacggg ataatcgtaa ccaattgaat ttggtttgat
     7681 ttgtaggccg cacgccacat ccgacattca gcgcctgatg cgacgcttga cgcgtcttat
     7741 caggcctaca agtccccagg ccagaaccgt aggcaggatc atccggcaac acgttattgg
     7801 caatctgcgt gaatgtttgt caccagtgcc tccagcacca ctttccagtc atcaaccacg
     7861 ccaacatctg cctgtgaaaa taccgcagcg ctggcgtcat gattaatcgc caccacaaac
     7921 ttgctattgc gtacgcccgc cattaacgct gctgcgccag aagcgccaac aacaatacac
     7981 acctcaggtg ccagcagatg tccggaaata ccaatcactt tttcagcatc aacgccaccg
     8041 ttcatcactc gcgcccgact gtagcccact tcggctccca gcttttctgc cagcatagcg
     8101 atttcctggt tatctgcctc tccgccctgc cccaccacca gcacccgtct ggcctcagct
     8161 aatgggtcgc gggtaacatt tttcagatcc tctgtgctaa ccagccagtc cggaagcgca
     8221 cccgggacaa tgttcagttg ttgcattcca gagggcaacg tggcgttttt cgctgctccc
     8281 gcctgacgcg ccagggaaag gcatagcggg cgtttttctg tttgcagcgt cgccgttagc
     8341 gcatttcccc agtgggattt gcgcacgctt acggttggaa tatctaacga agtcacctgg
     8401 cagatgcttg caccatgtaa acgccaggcc agccgggtcg acagttcatc gccaaacgtt
     8461 cccggcggaa agagaacaac gtccgcaggc gttcgctgcc actgttccac caacgcatcc
     8521 agcacctgtt ccgccactac aggctgaggt tcaatctgcc agtgcgccag ggtacaaccg
     8581 ctgaaatcct gcgccgccag ccagcttgct attgcggcat tctcctgatt tattgtcaca
     8641 attgcgatat tcatggctgc atcctttggc gtaaatagtc ccgccatagc ttttgagctt
     8701 tttctgctac cgtttgccca tcaatcagcg tcgctccgcg tcgttgctct gctcttgcca
     8761 gttgcagaca ctgcatcgct ggcatttcgg cagcaacagt tttacggata atttctgctt
     8821 tccccgcagc cattcgctgg cgcattcccg gtactggcaa ggccacttca ccgcactgac
     8881 gcacggcaat caccgcaggc aagcgaaccc ggcaacaacg caacccatgt tcagtacgct
     8941 gttcgagggt gataaacagc gcgtcgagag tgaaacgctc cacctgggtg aagcagggcc
     9001 agcccagcat ttccgccagc aaaaatggcg tttgcccatt ctgcccttcg ctgctttggc
     9061 agccggtaat tatcagatcc agcggattct gatgctgcca ttcggcaata tggcgagcga
     9121 caaattccgg cgcaaagcgc agatctgccg ccgtctccag caatacagct tcctcaaacc
     9181 caagagccat gagatagcgc agccagtgca atgcccgttc atcccccatg ctcaacgcgg
     9241 ttaaagacat cggcgtaccg ttttttctct gcgccaacag cagcgcggca gcggcctgtt
     9301 catcagcacc gagtaaactt cgcagtagcg aaatatccgg tccgctttta ccctgagccg
     9361 ccgcctgcca ctctttttcc gccagcattc cggcatccgg ttcggcttta aacgctaaca
     9421 gaatgttcat tcgcacctcc tacatttccc gccgccacca gcgggagggc tttagtttct
     9481 ggtgcccata gccaggtcac aacaaaacca accaacaaca ctgtcgccag gagcaataag
     9541 gtgacttgca ttccccactg cgccagcacc cacggcagca ggccagtact taccgccgcg
     9601 ccaagtcgac tcatggcggt ggcaaaaccg acgcccagcg agcgaatgtc agtaggaaaa
     9661 ctttccgcag gcaaaatgcc caccagatta ctgactgccg aaatggtggt gctgaagaga
     9721 acaaaaagca gcagcgttaa tgaactgccg gaaggcaaac aggccattac taccagcgtt
     9781 gccgccagca gcaaaaaact tcccagcaaa aatttgcgat gtgccagcag gtgcgtcaga
     9841 actaatccca gcagcgcgcc cacaattaac aacgcattaa gcatcaggct ggcagtcagc
     9901 gcatcttcca gaccaatagt ctgggcgata gttggcagcc aggtataaat cacaaaccat
     9961 gggattacga ggcagacaaa gaagacgctg ttaaacgccg tgcgccgcca gtaacgcgaa
    10021 gagaacaagg ttttgatgtg tttatgggtc gccgttacca cttcatcgcc cagtaaaaca
    10081 tggggaccaa aatagcgatg cacgatagcg tgagcttctg caaaacgccc ctggcgcagt
    10141 agccagcgtg gtgattctgg cgttccccag cgtaataacg taatcaacaa cgcgggcaga
    10201 gctgccgatg ccagtagcca gcgccaggcc tccgggtttt cggaaataaa gtgatgtccg
    10261 gcaatacttg ccagcacata gcctacggtc cacaccacgc tgaatgcgcc cagcaaaata
    10321 ccgcgatggc ggcgcgggga aaattcagcc agcaaggtgt gacctactga ataatcgcct
    10381 cccagaccaa tgccaatcaa aatgcgcagt ccaataagat gctctggcgt ggtggcaaaa
    10441 aattgtaaaa acgaagcaag cgtaatcagc aaaaagctga aggtgaagat tttttgccga
    10501 ccaatatggt cggagatcca cccaagaacc aggctgccaa ggaacaaacc aaggagcgcc
    10561 gagccgccga tcattcccgc cataaacggc gtcagttgca tggcgggcgt aagctgaata
    10621 atggcgtaac caatgacgcc gagaacataa ccgtcggtca ggtgtgcgcc gaaagtgagc
    10681 gcagcaatgc ggcagtgaaa acggttaagc ggtaaatcat ccatttgcac cggtgaagtg
    10741 ttcatcttta ccccttcatt gtccggtaat aagaacggct tctcgcctga gaagccgaaa
    10801 aggcaacgcc ccgtcatcgc ggaacgctgc ctgacacatt atttttcaat cggatagata
    10861 gtacccgtat tcataatgcc attaggatcg aactgctttt tcagcccttc cagcaacgcc
    10921 cacgcgctgc cgtgttccag tttgctccag tgaacgcgat gtttaccgat accgtggtgg
    10981 tgcaccatcg aaccgccgag gcgaatggtt tcttcacaga tgatcttgtt gagcggattg
    11041 tggtacttgt cgatttcctc ttccggctta cagtcaacga cgttgtaatc gtagacgaag
    11101 tacatgttgg tgccgttctg atagctatga gaggaatgac cgcccagcat ggtgatgtcg
    11161 tcggcgtgcg ggaactcagt acgaatacgg ttaataacgc tttcgtagat ttcgtggatg
    11221 cagctccagc agccggacac ttcagtggta aagcccatgt tgccggtttt gaggatctgc
    11281 acacgttcgg cagccacttt atccggtccc cagttcaggt tgttaaacca ggtttcgatc
    11341 agtttgctgt ccacgcgctg gcactgcggg tagcgggcaa cgatttccgc aatcccttcg
    11401 cccgtcgcct tcgcaatgcg aggattgcct tcagccataa agatcagcac acatttaccg
    11461 tcggcaaaat gggtgaagtg ttgggtgcca tcttcagcgt catacaaacg agcgatcgac
    11521 ggacgatacc cttccaccat gacttcacgc aggatgttga agccggtttt catgtcttcc
    11581 aggatatagc cgtagaagag gttgttttcc ggggtgaatt taaagatttt cactgttact
    11641 tcagtgatat agcaaagtgc accttcgtta ccgatgatga tgtggcgaat gtccgggcca
    11701 gccgcgcggc gtggcacgtt tttaatgcgt gtgacggtgc catcagccaa tactgcttcc
    11761 agaccaacga ccatatcttc gattgcgccg tagagtgtgg agaactgccc gatactgcgg
    11821 gttgctacca ggccgcccat ctgcgccagc ggctttgact gcggagaatg ccccgtggtg
    11881 taaccttttt cacgcaacgc attttccagc acctccagcg gaacgccaca ttgcgccgtc
    11941 gcctgcatat tctcaatatc aatattaatg atttgattca tggcggagcc gtcgagcacc
    12001 accgagtttt ccacaacggt ttccagccca ccttcagtgg cggaagcgcc ggtacgcggc
    12061 acaccgttaa ttttgtgcgc attcataaaa ttcagcacac gggagacttg ttctgtggaa
    12121 ccgagtttta cgaccgctgc cggaatcggc aaagtataaa taccatgaat atccggaaat
    12181 ttacgaaaac ggtcaatact gttcttcttt aatactgttt catcggtaat tacgcgatct
    12241 gcaccaacaa tttcctttag ctggtcgaca atcgctgcgc gagataaaga catagtaatt
    12301 ccttcctgat aataaaaatg gaataatcat taataagaga aaacagaaac caccgttatg
    12361 gattagcgca ctaaataacc accatcgacc actaataaat gcccattgac ataatttgaa
    12421 gccggacttg cgaggaatac agctgcgccc attaaatcct gagtatcgcc ccaacggttt
    12481 gccggaatat gatcaagaac gcgctgattg gtttctggat tactgcgtgt cgccagcgta
    12541 atatctgttg cataatagcc aggggcaata ccatttacct gaatattata ttgacctagt
    12601 tcatcacaat aagctttggt gaatccggca agagcatgtt tagtggcaga atatgcaggt
    12661 gaccattgtc caccta
//
LOCUS       DAZKIV010000136        12622 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZKIV010000136 DAZKIV010000000
VERSION     DAZKIV010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.136"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(88..1371)
                     /gene="gltA"
                     /locus_tag="ACHOT6_003718"
     CDS             complement(88..1371)
                     /gene="gltA"
                     /locus_tag="ACHOT6_003718"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFP9948873.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1577..1684)
                     /locus_tag="ACHOT6_003719"
     CDS             complement(1577..1684)
                     /locus_tag="ACHOT6_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948874.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2065..2469
                     /gene="sdhC"
                     /locus_tag="ACHOT6_003720"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2065..2469
                     /gene="sdhC"
                     /locus_tag="ACHOT6_003720"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9948875.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2463..2810
                     /gene="sdhD"
                     /locus_tag="ACHOT6_003721"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2463..2810
                     /gene="sdhD"
                     /locus_tag="ACHOT6_003721"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFP9948876.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2810..4576
                     /gene="sdhA"
                     /locus_tag="ACHOT6_003722"
     CDS             2810..4576
                     /gene="sdhA"
                     /locus_tag="ACHOT6_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFP9948877.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEVSLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4592..5308
                     /gene="sdhB"
                     /locus_tag="ACHOT6_003723"
     CDS             4592..5308
                     /gene="sdhB"
                     /locus_tag="ACHOT6_003723"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFP9948878.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5609..8410
                     /gene="sucA"
                     /locus_tag="ACHOT6_003724"
     CDS             5609..8410
                     /gene="sucA"
                     /locus_tag="ACHOT6_003724"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFP9948879.1"
                     /translation="MQNSALKAWLDSSYLSGTNQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDSTEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8425..9642
                     /gene="odhB"
                     /locus_tag="ACHOT6_003725"
     CDS             8425..9642
                     /gene="odhB"
                     /locus_tag="ACHOT6_003725"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFP9948880.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9736..10902
                     /gene="sucC"
                     /locus_tag="ACHOT6_003726"
     CDS             9736..10902
                     /gene="sucC"
                     /locus_tag="ACHOT6_003726"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFP9948881.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10902..11771
                     /gene="sucD"
                     /locus_tag="ACHOT6_003727"
     CDS             10902..11771
                     /gene="sucD"
                     /locus_tag="ACHOT6_003727"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFP9948882.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11875..12597)
                     /gene="mngR"
                     /locus_tag="ACHOT6_003728"
     CDS             complement(11875..12597)
                     /gene="mngR"
                     /locus_tag="ACHOT6_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFP9948883.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
BASE COUNT         3028 a         3224 c         3425 g         2945 t
ORIGIN      
        1 ttttacaatt ttttgtgttt aggcatataa aaattaaccc gccatttgaa cggcgggtta
       61 aaatatttac aacttagcaa tcaaccatta acgcttgata tcgcttttaa agtcgcgttt
      121 ttcatatcct gtatacagct gacgcggacg ggcaatcttc ataccgtcac tgtgcatttc
      181 gctccagtgg gcgatccagc caacggtacg tgccattgcg aaaatgacgg tgaacatgga
      241 agacggaata cccatcgctt tcaggatgat accagagtag aaatcgacgt tcgggtacag
      301 tttcttctcg ataaagtacg ggtcgttcag cgcgatgttt tccagctcca tagccacttc
      361 cagcaggtca tctttggtgc ccagctcttt cagaacttca tggcaggttt cacgcattac
      421 ggtggcgcgc gggtcgtaat ttttgtacac gcggtgaccg aagcccatca ggcggaaaga
      481 atcattttta tctttcgcac gacgaacaaa ttccggaatg tgtttaacgg agctaatttc
      541 ttccagcatt ttcagcgccg cttcgttagc accaccgtgc gcaggtcccc acagtgaagc
      601 aatacctgct gcgatacagg caaacgggtt cgcacccgaa gagccagcgg tacgcacggt
      661 ggaggtagag gcgttctgtt catggtcagc gtgcaggatc agaatacggt ccatagcacg
      721 ttccagaatc ggattaactt catacggttc gcacggcgtg gagaacatca tattcaggaa
      781 gttaccggca taggagagat cgttgcgcgg ataaacaaat ggctgaccaa tggaatactt
      841 gtaacacatc gcggccatgg tcggcatttt cgacagcagg cggaacgcgg caatttcacg
      901 gtgacgagga ttgttaacat ccagcgagtc gtgatagaac gccgccagcg cgccggtaat
      961 accacacatg actgccattg gatgcgagtc gcgacggaaa gcgtggaaca gacgggtaat
     1021 ctgctcgtgg atcatggtat gacgggtcac cgtagtttta aattcgtcat actgttcctg
     1081 agtcggtttt tcaccattca gcaggatgta acaaacttcc aggtagttag aatcggtcgc
     1141 cagctgatcg atcgggaaac cgcggtgcag caaaatacct tcatcaccat caataaaagt
     1201 aattttagat tcgcaggatg cggttgaagt gaagcctggg tcaaaggtga acacaccttt
     1261 tgaaccgaga gtacggatat caataacatc ttgacccagc gtgcctttca gcacatccag
     1321 ttcaacagct gtgtccccgt tgagggtgag ttttgctttt gtatcagcca tttaaggtct
     1381 ccttagcgcc ttattacgta agactgccgg aacttaaatt tgccttcgca catcaacctg
     1441 gctttacccg tttgttattt ggctcgccgc tctgtgaaag aggggaaaac ctgggtacag
     1501 agctctgggc gcttgcaggt aaaggatcca ttgatgacga ataaatggcg aatcaagtac
     1561 ttagcaatcc gaattattaa acttgtctac cactaataac tgtcccgaat gaattggtca
     1621 atacttccac actgttacat aagttaatct taggtgaaat accgacttca taacttttac
     1681 gcattatatg cttttcctgg taatgtttgt aacaactttg ttgaatgatt gtcaaattgg
     1741 atgattaaaa attaaataaa tgttgttatc gtgacctgga tcactgttca ggataaaacc
     1801 cgacaaacta tatgtaggtt aattgtaatg attttgtgaa cggcctatac tgccgccagg
     1861 tctccggaac accctgcaat cccgagccac ccagcgttgt aacgtgtcgt tttcgcatct
     1921 ggaagcagtg ttttgcatga cgcgcagtta tagaaaggac gctgtctgac ccgcaagcag
     1981 accggaggaa ggaaatcccg acgtctccag gtaacagaaa gttaacctct gtgcccgtag
     2041 tccccaggga ataataagaa cagcatgtgg gcgttattca tgataagaaa tgtgaaaaaa
     2101 caaagacctg ttaatctgga cctacagacc atccggttcc ccatcacggc gatagcgtcc
     2161 attctccatc gcgtttccgg tgtgatcacc tttgttgcag tgggcatcct gctgtggctt
     2221 ctgggtacca gcctctcttc ccctgaaggt ttcgagcaag cttccgcgat tatgggcagc
     2281 ttcttcgtca aatttatcat gtggggcatc cttaccgctc tggcgtatca cgtcgtcgta
     2341 ggtattcgcc acatgatgat ggattttggc tatctggaag aaacattcga agcgggtaaa
     2401 cgctccgcca aaatctcctt tgttattact gtcgtgcttt cacttctcgc aggagtcctc
     2461 gtatggtaag caacgcctcc gcattaggac gcaatggcgt acatgatttc atcctcgttc
     2521 gcgctaccgc tatcgtcctg acgctctaca tcatttatat ggtcggtttt ttcgccacca
     2581 gtggcgaact gacatatgaa gtctggatcg gtttcttcgc ctctgcgttc accaaagtgt
     2641 tcaccctgct ggcgctgttt tctatcttga ttcatgcctg gatcggcatg tggcaggtgt
     2701 tgaccgacta cgttaaaccg ctggcattgc gcctgatgct gcaactggtg attgtcgttg
     2761 cactggtggt ttacgtgatt tatggattcg ttgtggtgtg gggtgtgtga tgaaattgcc
     2821 agtcagagaa tttgatgcag ttgtgattgg tgccggtggc gcaggtatgc gcgcggcgct
     2881 gcaaatttcc cagagcggcc agacctgtgc gctgctctct aaagtcttcc cgacccgttc
     2941 ccataccgtt tctgcgcaag gcggcattac cgttgcgctg ggtaataccc atgaagataa
     3001 ctgggaatgg catatgtacg acaccgtaaa agggtcggac tatatcggtg accaggacgc
     3061 gattgaatat atgtgtaaaa ccgggccgga agcgattctg gaactcgaac acatgggcct
     3121 gccgttctcg cgtcttgatg atggtcgtat ctatcaacgt ccgtttggcg gtcagtcgaa
     3181 aaacttcggc ggcgagcagg cggcacgcac tgcggcagcg gctgaccgta ccggtcacgc
     3241 actgttgcac acgctttatc agcagaacct gaaaaaccac accaccattt tctccgagtg
     3301 gtatgcgctg gatctggtga aaaaccagga tggcgcggtg gtggggtgta ccgcactgtg
     3361 catcgaaacc ggtgaagtag tttatttcaa agcccgcgct accgtgctgg cgactggcgg
     3421 ggcagggcgt atttatcagt ccaccactaa cgcccacatt aacactggcg acggtgtcgg
     3481 catggctatc cgcgcaggcg taccggtgca ggatatggaa atgtggcaat tccacccgac
     3541 cggcattgcc ggtgcgggcg tactggtcac cgaaggttgc cgtggtgaag gcggttatct
     3601 gctgaacaaa catggcgaac gctttatgga gcgttatgcg ccgaacgcca aagacctggc
     3661 gggccgtgat gtggtggcgc gttccatcat gatcgaaatc cgtgaaggtc gcggctgtga
     3721 tggtccgtgg gggccacacg cgaaactgaa actcgaccac ctgggtaaag aagttctcga
     3781 atcccgtctg ccgggtatcc tggagctttc ccgtaccttc gctcacgtcg atccggtgaa
     3841 agagccgatt ccggttatcc caacctgtca ctacatgatg ggcggtattc cgaccaaagt
     3901 taccggtcag gcgctgactg tgaatgagaa aggcgaagat gtggttgttc cgggactgtt
     3961 tgccgttggt gaaatcgctt gtgtatcggt acacggcgct aaccgtctgg gcggcaactc
     4021 gctgctggac ctggtggtct ttggtcgcgc ggcaggtctg catctgcaag agtctatcgc
     4081 cgagcagggc gcactgcgcg atgccagcga gtctgatgtt gaagtgtctc tggatcgcct
     4141 gaaccgctgg aacaataatc gtaacggtga agatccggtg gcgattcgta aagcgctgca
     4201 agaatgtatg cagcataact tctcggtctt ccgtgaaggt gatgcgatgg cgaaagggct
     4261 tgagcagttg aaagtgatcc gcgagcgtct gaaaaatgcc cgtctggatg acacttccag
     4321 cgagttcaac acccagcgcg ttgagtgcct ggaactggat aacctgatgg aaacggcgta
     4381 tgcaacggct gtttctgcca acttccgtac cgaaagccgt ggcgcacata gccgcttcga
     4441 cttcccggat cgtgatgatg aaaactggct gtgccactcc ctgtatctgc ctgagtcgga
     4501 atccatgacg cgccgaagcg tcaacatgga accgaaactg cgcccggcat tcccgccgaa
     4561 gattcgtact tactaatgcg gagacaggaa aatgagactc gagttttcaa tttatcgcta
     4621 taacccggat gttgatgatg ctccgcgtat gcaggattac accctggaag cggaagaagg
     4681 tcgcgacatg atgctgctgg atgcgcttat ccagctgaaa gagaaagatc ccagcctgtc
     4741 gttccgccgc tcctgtcgtg aaggtgtgtg cggttccgac ggtctgaaca tgaacggcaa
     4801 gaatggtctg gcctgtatta ccccgatttc ggcactcaac cagccgggca agaagattgt
     4861 gattcgcccg ctgccaggtt taccggtgat ccgcgatttg gtggtagaca tgggacaatt
     4921 ctatgcgcaa tatgagaaaa ttaagcctta cctgttgaat aatggacaaa atccgccagc
     4981 tcgcgagcat ttacagatgc cagagcagcg cgaaaaactc gacgggctgt atgaatgtat
     5041 tctctgcgca tgttgttcaa cctcttgtcc gtctttctgg tggaatcccg ataagtttat
     5101 cggcccggca ggcttgttag cggcatatcg tttcctgatc gatagccgtg ataccgagac
     5161 tgacagccgc ctcgacggtt tgagcgatgc attcagcgta ttccgctgtc acagcatcat
     5221 gaactgcgtc agtgtatgtc cgaaggggct gaacccgacg cgcgccatcg gccatattaa
     5281 gtcgatgttg ttgcaacgta atgcgtaaac cgtaggcctg ataagacgcg caagcgtcgc
     5341 atcaggcaac cagtgccgga tgcggcgtga acgccttatc cggcctacaa gtcattaccc
     5401 gtaggcctga taagcgcagc gcatcaggcg taacaaagaa atgcaggaaa tctttaaaaa
     5461 ctgcccctga cactaagaca gtttttaaag gttccttcgc gagccactac atagacaaga
     5521 gctcgcaagt gaaccccggc acgcacatca ctgtgcgtgg tagtatccac ggcgaagtaa
     5581 gcataaaaaa gatgcttaag ggatcacgat gcagaacagc gctttgaaag cctggttgga
     5641 ctcttcttac ctctctggca caaaccagag ctggatagaa cagctctatg aagacttctt
     5701 aaccgatcct gactcggttg acgctaactg gcgttcgacg ttccagcagt tacctggtac
     5761 gggagtcaaa ccggatcaat tccactctca aacgcgtgaa tatttccgcc gcctggcgaa
     5821 agacgcttca cgttactctt caacgatctc cgaccctgac accaatgtga agcaggttaa
     5881 agtcctgcag ctcattaacg cataccgctt ccgtggtcac cagcatgcga atctcgatcc
     5941 gctgggactg tggcagcaag ataaagtggc cgatctggat ccgtctttcc acgatctgac
     6001 cgaagcagac ttccaggaga ccttcaacgt cggttcattt gccagcggca aagaaaccat
     6061 gaaactcggc gagctgctgg aagccctcaa gcaaacctac tgtggcccga ttggtgccga
     6121 gtatatgcac attaccagca ccgaagaaaa acgctggatc caacagcgta tcgagtctgg
     6181 tcgcgcgact ttcaatagcg aagagaaaaa acgcttctta agcgaactga ccgccgctga
     6241 aggccttgaa cgttacctcg gcgcaaaatt ccctggcgca aaacgcttct cgctggaagg
     6301 cggtgacgcg ttaatcccga tgcttaaaga gatgatccgc cacgctggca acagcggcac
     6361 ccgcgaagtg gttcttggga tggcgcaccg tggtcgtctg aacgtgctgg tgaacgtgct
     6421 gggtaaaaaa ccgcaagact tgttcgacga gtttgccggt aaacataaag aacacctcgg
     6481 cacgggtgac gtgaaatacc acatgggctt ctcgtctgac ttccagaccg atggcggcct
     6541 ggtgcacctg gcgctggcgt ttaacccgtc tcaccttgag attgtaagcc cggtagttat
     6601 cggttctgtt cgtgcccgtc tggacagact tgatgagccg agcagcaaca aagtgctgcc
     6661 aatcaccatc cacggtgacg ccgcagtgac cgggcagggc gtggttcagg aaactctgaa
     6721 catgtcgaaa gcgcgtggtt atgaagttgg cggtacggta cgtatcgtta tcaacaacca
     6781 ggttggtttc accacctcta atccgctgga tgctcgttct acaccgtact gtactgatat
     6841 cggtaagatg gttcaggcac cgattttcca cgttaacgcg gatgatccgg aagccgttgc
     6901 ctttgtgacc cgtctggcgc tcgatttccg taacaccttt aaacgtgatg tcttcatcga
     6961 cctggtgtgc taccgccgtc acggccacaa cgaagccgac gagccgagcg caacccagcc
     7021 gctgatgtat cagaaaatca aaaaacatcc gacaccgcgc aaaatctacg ctgacaagct
     7081 ggagcaggaa aaagtggcga cgctggaaga ttccaccgag atggttaacc tgtaccgcga
     7141 tgcgctggat gctggcgatt gcgtagtggc agagtggcgt ccgatgaaca tgcactcttt
     7201 cacctggtcg ccgtacctca accacgaatg ggacgaagag tacccgaaca aagttgagat
     7261 gaagcgcctg caggagctgg cgaaacgcat cagcacggtg ccggaagcag ttgaaatgca
     7321 gtctcgcgtt gccaagattt atggcgatcg ccaggcgatg gctgccggtg agaaactgtt
     7381 cgactggggc ggtgcggaaa acctcgctta cgccacgctg gttgacgaag gcattccggt
     7441 tcgcctgtcg ggtgaagact ccggtcgcgg taccttcttc caccgccacg cggtgatcca
     7501 caaccagtct aacggttcca cttacacgcc gctgcaacat attcataacg gccagggcgc
     7561 gttccgtgtc tgggactccg ttctgtctga agaagcagtg ctggcgtttg aatacggtta
     7621 tgccaccgca gaaccacgca ctctgactat ctgggaagcg cagttcggtg acttcgccaa
     7681 cggtgcgcag gtggttatcg accagttcat ctcctctggc gagcagaaat ggggccggat
     7741 gtgtggtctg gtgatgttgc tgccgcacgg ttacgaaggg caggggccgg agcactcctc
     7801 cgcgcgtctg gaacgttatc tgcaactttg tgctgagcaa aacatgcagg tgtgcgtacc
     7861 gtctaccccg gcacaggttt accacatgct gcgtcgtcag gcgctgcgcg ggatgcgtcg
     7921 tccgctggtc gtgatgtcgc cgaaatccct gctgcgtcat ccgctggcgg tttccagcct
     7981 cgaagaactg gcgaacggca ccttcctgcc agccatcggt gaaatcgacg agcttgatcc
     8041 gaagggcgtg aagcgcgtag tgatgtgttc tggtaaggtt tattacgacc tgctggaaca
     8101 gcgtcgtaag aacaatcaac acgatgtcgc cattgtgcgt atcgagcaac tctacccgtt
     8161 cccgcataaa gcgatgcagg aagtgttgca gcagtttgct cacgtcaagg attttgtctg
     8221 gtgccaggaa gagccgctca accagggcgc atggtactgc agccagcatc atttccgtga
     8281 agtgattccg tttggggctt ctctgcgtta tgcaggccgc ccggcctccg cctctccggc
     8341 ggtagggtat atgtccgttc accagaaaca gcaacaagat ctggttaatg acgcgctgaa
     8401 cgtcgaataa ataaaggata cacaatgagt agcgtagata ttctggtccc tgacctgcct
     8461 gaatccgtag ccgatgccac cgtcgcaacc tggcataaaa aacccggcga cgcagtcgta
     8521 cgtgatgaag tgctggtaga aatcgaaact gacaaagtgg tactggaagt accggcatca
     8581 gcagacggca ttctggatgc ggttctggaa gatgaaggta caacggtaac gtctcgtcag
     8641 atccttggtc gcctgcgtga aggcaacagc gccggtaaag aaaccagcgc caaatctgaa
     8701 gagaaagcgt ccactccggc gcaacgccag caggcatctc tggaagagca aaacaacgat
     8761 gcgttaagcc cggcgatccg tcgcctgctg gctgaacaca atctcgacgc cagcgccatt
     8821 aaaggcaccg gtgtgggtgg tcgtctgact cgtgaagatg tggaaaaaca tctggcgaaa
     8881 gccccggcga aagagtctgc tccggcagcg gctgctccgg cggcgcaacc ggctctggct
     8941 gcacgtagtg aaaaacgtgt cccgatgact cgcctgcgta agcgtgtggc agagcgtctg
     9001 ctggaagcga aaaactccac cgccatgctg accacgttca acgaagtcaa catgaagccg
     9061 attatggatc tgcgtaagca gtacggtgaa gcgtttgaaa aacgccacgg catccgtctg
     9121 ggctttatgt ccttctacgt gaaagcggtg gttgaagccc tgaaacgtta cccggaagtg
     9181 aacgcgtcta tcgacggcga tgacgtggtt taccacaact atttcgacgt cagcatggcg
     9241 gtttctacgc cgcgcggcct ggtgacaccg gtactgcgtg atgtcgatac cctcggcatg
     9301 gcagacatcg agaagaaaat caaagagctg gcagtcaaag gccgtgacgg caagctgacc
     9361 gttgaagatc tgaccggtgg taacttcacc atcaccaacg gtggtgtgtt cggttccctg
     9421 atgtctacgc cgatcatcaa cccgccgcag agcgcaattc tgggtatgca cgctatcaaa
     9481 gatcgtccga tggcggtgaa tggtcaggtt gagatcctgc cgatgatgta cctggcgctg
     9541 tcctacgatc accgtctgat cgatggtcgc gaatccgtgg gcttcctggt aacgatcaaa
     9601 gagttgctgg aagatccgac gcgtctgctg ctggacgtgt agtagtttaa gcttcacctg
     9661 cactgtagac cgacgtggat agggtgtcgg tctacggttt aaaagataac gattactgaa
     9721 ggatggacag aacacatgaa cttacatgaa tatcaggcaa aacaactttt tgcccgctat
     9781 ggcttaccag caccggtggg ttatgcctgt actactccgc gcgaagcaga agaagccgct
     9841 tcaaaaatcg gtgccggccc gtgggtagtg aaatgtcagg ttcacgctgg tggccgcggt
     9901 aaagcgggcg gtgtgaaagt tgtaaacagc aaagaagaca tccgtgcttt tgcagaaaac
     9961 tggctcggca agcgtctggt aacgtatcaa acagatgcca atggccaacc ggttaaccag
    10021 attctggttg aagcagcgac cgatatcgct aaagagctgt atctcggtgc cgttgtagac
    10081 cgtagttccc gtcgtgtggt ctttatggcc tccaccgaag gcggcgtgga aatcgaaaaa
    10141 gtggcggaag aaactccgca cctgatccac aaagttgcgc ttgatccgct gactggcccg
    10201 atgccgtatc agggacgcga gctggcgttc aaactgggtc tggaaggtaa actggttcag
    10261 cagttcacca aaatcttcat gggcctggcg accattttcc tggagcgcga cctggcgttg
    10321 atcgaaatca acccgctggt catcaccaaa cagggcgatc tgatttgcct cgacggcaaa
    10381 ctgggcgctg acggcaacgc actgttccgc cagcctgatc tgcgcgaaat gcgtgaccag
    10441 tcgcaggaag atccgcgtga agcacaggct gcacagtggg aactgaacta cgttgcgctg
    10501 gacggtaaca tcggttgtat ggttaacggc gcaggtctgg cgatgggtac gatggacatc
    10561 gttaaactgc acggcggcga accggctaac ttccttgacg ttggcggcgg cgcaaccaaa
    10621 gaacgtgtaa ccgaagcgtt caaaatcatc ctctctgacg acaaagtgaa agccgttctg
    10681 gttaacatct tcggcggtat cgttcgttgc gacctgatcg ctgacggtat catcggcgcg
    10741 gtagcagaag tgggtgttaa cgtaccggtc gtggtacgtc tggaaggtaa caacgccgaa
    10801 ctcggcgcga agaaactggc tgacagcggc ctgaatatta ttgcagcaaa aggtctgacg
    10861 gatgcagctc agcaggttgt tgccgcagtg gaggggaaat aatgtccatt ttaattgata
    10921 aaaacaccaa ggttatctgc cagggcttta ctggtagcca ggggactttc cactcagaac
    10981 aggccattgc atacggcact aaaatggttg gcggcgtaac cccaggtaaa ggcggtacta
    11041 cccacctcgg cctgccggtg ttcaacaccg tgcgtgaagc tgttgctgct actggcgcta
    11101 ccgcttctgt tatctacgta ccagcaccgt tctgcaaaga ctccattctg gaagctattg
    11161 atgcaggcat caaactgatt atcaccatca ctgaaggcat cccgacgctg gatatgctga
    11221 ctgtgaaagt gaaactggac gaagccggcg ttcgtatgat cggcccgaac tgcccaggcg
    11281 ttatcactcc gggtgaatgc aaaatcggta tccagccggg tcacattcac aaaccgggta
    11341 aagtgggtat cgtttcccgt tccggtacac tgacctatga agcggttaaa cagaccacgg
    11401 attacggttt cggtcagtcg acctgtgttg gtatcggcgg tgacccgatc ccaggctcta
    11461 actttatcga catcctcgaa atgttcgaaa aagatccgca gaccgaagcg atcgtgatga
    11521 tcggtgagat cggcggtagc gctgaagaag aagcggcagc gtacatcaaa gagcacgtta
    11581 ccaagccagt tgtaggttac atcgctggtg tgactgcgcc gaaaggcaaa cgtatgggcc
    11641 acgcgggtgc gatcattgcc ggtgggaaag ggactgcgga tgagaaattc gctgctctgg
    11701 aagccgcagg cgtgaaaacc gttcgcagcc tggcggatat cggtgaagca ctgaaaactg
    11761 ttctgaaata aatatctgta ataagaaata gccctcgccg cttccctcta caggaatggc
    11821 gaagggctgt cggtttcgac atggttggcc atcgtatgat ggcctttttt gtgcttatcg
    11881 cgatgatttt cgctgcgcta tcagggtaaa tttatagtca tcggtattaa aagcgttgcg
    11941 gctatattca aacacccgac catcaactaa atatccacgc gatacttttt caagaatcgg
    12001 ctttgtctgg ctgatattaa gcagacggct catctcttcg gttggcatca gaggaatgat
    12061 ttcctgttcg ctacgatcga taaccatctt cttcacttct tcaataaagt gatatttcga
    12121 attttccatg acctgccagg tgagatccgg gaacaacgca agcggcatcc aggtttcttc
    12181 cagcgccatt ggcttttgct tgcgatagcg cacgcgcttc acatgccaca cacgatcctg
    12241 cgcggtgatt tgtagctgtt gctgaagaaa atcgtcagcc ggaatcactt caaatatcag
    12301 gacttcactg tgtgtatcga cgtgacggtc cgacagtttt tcatcaaaac tggttaactg
    12361 aaaaatatcg taattgaccc gctcttcttt gacgtaagtc ccgctgccct gaatgctttc
    12421 gaggatctgt tgctcgacta actggcgcaa agcctgacgc accgtaaccc ggctgacgcc
    12481 aaactctgtt tgtaatgccg attcggtagg taacgcatcg ccaggtttaa gctcgccacg
    12541 cgcaatttgt tcacgaatgc gatcggcaat ctgccggtat aagggcttgt gtcccatttt
    12601 tagtatctca ttaatacgaa tt
//
LOCUS       DAZKIV010000137        12605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZKIV010000137 DAZKIV010000000
VERSION     DAZKIV010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.137"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            20..343
                     /gene="yiaW"
                     /locus_tag="ACHOT6_003729"
     CDS             20..343
                     /gene="yiaW"
                     /locus_tag="ACHOT6_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9948884.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            349..1485
                     /gene="yiaV"
                     /locus_tag="ACHOT6_003730"
     CDS             349..1485
                     /gene="yiaV"
                     /locus_tag="ACHOT6_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9948885.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVNSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFIHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(1482..2456)
                     /gene="yiaU"
                     /locus_tag="ACHOT6_003731"
     CDS             complement(1482..2456)
                     /gene="yiaU"
                     /locus_tag="ACHOT6_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9948886.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
     gene            2580..3320
                     /gene="yiaT"
                     /locus_tag="ACHOT6_003732"
     CDS             2580..3320
                     /gene="yiaT"
                     /locus_tag="ACHOT6_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFP9948887.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(3441..5420)
                     /locus_tag="ACHOT6_003733"
     CDS             complement(3441..5420)
                     /locus_tag="ACHOT6_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003581012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HFP9948888.1"
                     /translation="MNISEVDLHKLTVSDPFLGQYQQLVRDVVIPYQWDALNDRIPEA
                     EPSHAIENFRIAAGLQEGEFYGMVFQDSDVAKWLEAVAWSLCQKPDAELEKTADEVIE
                     LIASAQCEDGYLNTYFTVKAPEERWSNLAECHELYCAGHLIEAGVAFFQATGKRRLLE
                     VVCRLADHIDRVFGPDEDKLQGYPGHPEIELALMRLYEVTEEPRYLALTNYFVEQRGV
                     QPHYYDQEYEKRGQTSHWHTYGPAWMVKDKAYSQAHLPLAQQQTAIGHAVRFVYLMTG
                     VAHLARLSHDDSKRQDCLRLWNNMAQRQLYITGGIGSQSSGEAFSSDYDLPNDTVYAE
                     SCASIGLMMFARRMLEMEGDSQYADVMERALYNTVLGGMALDGKHFFYVNPLEVHPKS
                     LKFNHIYDHVKPIRQRWFGCACCPPNIARVLTSIGHYLYTPREDALYINIYAGNSMEV
                     PVENGTLRLRVSGNYPWQEQVTIAVESPQPVRHTLALRLPDWCTQPQIILNGEEVEQD
                     IRKGYLHITREWQEGDTLNLTLPMPVRRVYGNPLVRHVAGKVAIQRGPLVYCLEQADN
                     GESLHNLWLPTDAPFTTFEGKGLFSHKILIQAPGYRYEQSNPEQQPLWHYDSAPAKRQ
                     PQTLTFIPWFSWANRGEGEMRIWVNEEKHRHPEVG"
     gene            complement(5431..6831)
                     /locus_tag="ACHOT6_003734"
     CDS             complement(5431..6831)
                     /locus_tag="ACHOT6_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9948889.1"
                     /translation="MTSTPITTADIANGKIDDALSVREKIGYGLGDAGGTVITCLIMN
                     FLTFFYTDVFGLTPALVGTLFIALRVFDAISDPVMGVIADRTQSRWGRFRPWQLWIAI
                     PIGIIGILTFTVPDASMGVKIAWAFGTYLLLSVGYTAINVPYCALINTMTTRHNEVIS
                     CQSWRFVLCGVAGFLVSVGLPWMVDLFGQGNAARGYQLGVGVLCAIAVVMFLCCFFWV
                     RERVPLSTMGKFTLREHLAGLRNNDQLLLMLVMSFLLINVFNIRGGGYMYFITYVLQG
                     STGYTSLFFTMVTFASIIGSVIVSPLTRRFDTVKIYYYTNLLLAALAVLMWFLPSGPA
                     YQTLWLAVILGNGVILGFTLPLHFSLMAFADDYGEWKTRVRSSGMNFAFNLFFIKLAW
                     ASSAGIISLLFIFVAYQPGVENQTASSLGGIAAMETLLPALFHLLLAGAIRFCKLNNP
                     MMSRIATDLRQRHVQP"
     gene            7057..7872
                     /locus_tag="ACHOT6_003735"
     CDS             7057..7872
                     /locus_tag="ACHOT6_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9948890.1"
                     /translation="MLELSMTLPIKVQNGGLFISRGVGRHPARRLESWEIIFVEKGRL
                     TIQEEERIFLVDAGESLLLWPNRQHVGVEDFPADLKFYWLHFEVKAPDSDPRWLTHLS
                     VPQQTKVADPQALVALFRQFLSEQEKHQRTPALEFILLLILQQLTRDARLDEQADGAG
                     TTLAWKAQKLIRTQYHRPLSTALLARELYCNADYLGRVYRRVFHLTLTEAIHRQRVRE
                     AEKLLISEARSLTEVAALCGFNDVGYFRQIFRKHTGLTPAAWKRRYSKEHINS"
     gene            complement(7904..8599)
                     /gene="araD"
                     /locus_tag="ACHOT6_003736"
     CDS             complement(7904..8599)
                     /gene="araD"
                     /locus_tag="ACHOT6_003736"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFP9948891.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRHAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGDNAYYGQ"
     gene            complement(8593..9453)
                     /gene="sgbU"
                     /locus_tag="ACHOT6_003737"
     CDS             complement(8593..9453)
                     /gene="sgbU"
                     /locus_tag="ACHOT6_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFP9948892.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWSVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(9446..10108)
                     /gene="ulaD"
                     /locus_tag="ACHOT6_003738"
     CDS             complement(9446..10108)
                     /gene="ulaD"
                     /locus_tag="ACHOT6_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFP9948893.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALASSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(10105..11601)
                     /gene="lyxK"
                     /locus_tag="ACHOT6_003739"
                     /pseudo
     CDS             complement(10105..11601)
                     /gene="lyxK"
                     /locus_tag="ACHOT6_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
     gene            complement(11605..12591)
                     /gene="yiaO"
                     /locus_tag="ACHOT6_003740"
     CDS             complement(11605..12591)
                     /gene="yiaO"
                     /locus_tag="ACHOT6_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFP9948894.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNELLQEKTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         2907 a         3291 c         3368 g         3039 t
ORIGIN      
        1 tatattctct gagggaaata tgtttctgga ctattttgca ctgggagtgc ttatttttgt
       61 atttctggtg atcttctatg ggatcattat tttacatgat attccctacc tgattgccaa
      121 aaaacgtaat catcctcatg ccgacgccat tcatgttgct ggttgggtga gtctttttac
      181 gttacatgtt atctggccgt ttttgtggat ttgggccacg ctttaccgcc cggagcgggg
      241 atggggaatg caaagccatg attcatccgt tatgcaactg caacagcgca ttgccgggct
      301 ggagaaacag ctcgccgaca tcaaatcctc ttctgccgag taatacttat ggatttactg
      361 attattttga cctatgtggc ttttgcatgg gcaatgttta agatcttcaa aattcccgta
      421 aataaatgga ccattcccac agcggccctg ggtggaatat ttattgtcag cggtctaatt
      481 ctgttaatga actataacca tccgtatacc tttaaagcgc aaaaagcggt tatttctatt
      541 cctgttgtcc cacaggtgac aggcgtggtg atcgaagtga cggataagaa aaatacgctg
      601 attaaaaaag gtgaggtgct atttcgactg gacccgacgc gttatcaggc gcgggtggat
      661 cggctgatgg cggatatcgt taccgcagaa cataaacagc gggcgttggg cgcagagtta
      721 gatgagatgg cggcgaatac tcagcaggca aaggccacgc gggataaatt cgctaaagag
      781 tatcagcgtt acgcacgcgg cagccaggcg aaagtaaacc cgttttcaga acgcgatatt
      841 gatgtggcgc ggcaaaatta tctggcgcag gaagcctccg taaattcatc ggcggcggaa
      901 caaaaacaga tccagagcca gctggatagc ctggtgttgg gtgaacattc tcaaatcgcc
      961 agcctgaaag cgcagctcgc ggaagcaaaa tataaccttg agcagacgat agtgcgtgcg
     1021 ccgagcgatg gttatgtgac ccaggtactg attcgtccgg gtacctatgc cgcgtcgctg
     1081 ccgctacgtc cggtgatggt gtttataccc gatcagaaac gacaaattgt ggcgcagttc
     1141 cgtcagaact ccttgctgcg actggcccct ggcgacgatg cggaagtggt gtttaatgct
     1201 ctgccaggta aggtattcag cggcaagctg gcagccatta gtccagctgt tcccggcgga
     1261 gcttatcagt cgaccggcac cttacagacg ttaaacacag cgccgggttc agatggcgtt
     1321 atcgcgacca ttgaactgga tgagcacact gatttgagcg ctttaccaga cggtatttac
     1381 gcccaggtgg cggtctactc tgatcatttc atccatgtct cggtgatgcg caaagtgcta
     1441 ttacgcatga ccagctgggt gcattacctt tatctcgatc attaatcttc gctttccgcc
     1501 gcttgttcag gccgcagttg ctgcgactga gcaagctggt ggcgaatttc acgcagcatt
     1561 tcatccagca catactggac ccgccagggc tggtattccc gtttacggaa gatagccacc
     1621 agatccagcc accattcata cgggtgctga aagcagggca ccagcgtgcc gtcttgcaga
     1681 tcgctctgca cacttttgtc tggcgcaaac acaatcccca ggtgatttcg tgccagctcc
     1741 agcgccgatt gcgtgttatc gcaaacgtaa tttcccttca cgcgataatc ccgcacctct
     1801 ttactcccgg cgacgttgaa gcgccagata ttcgcgtcgt caatcatcat cgagtcgatc
     1861 aaaatacagg agtgatgttc gagttcgtca ggacggctaa taggatgttt ctcaagatag
     1921 cgctggctgg cataagcggt tacggcatat tgtgtaataa aactggcaac cagcgattca
     1981 tcttttggtg gagcgtagct gattaaaaca tcacaatcat caggaaattc gacgccttca
     2041 taaaaggcat tacgctcaag attgcaggtt tttagcgata aggtgatatc accgatatct
     2101 ttaattttat cgattacatg tttggataaa taggtaatta tgccggttgg ggcgtagatg
     2161 gtgacccgac cacgtttctc atgcttataa tctgcaataa aattattaag ttgctcgttt
     2221 ctgtccagca tgtcgttgat gtacggtaac agcgcggtgc caaaaggtgt gagcatcagc
     2281 tgccgggtgg ttcggtcaaa gacttttaaa cccacttttg attcaaaatc agcaagatat
     2341 ttgctgacgt tggcctgtgc gatgccaagt acagtcgcgg catggctgat attttcactg
     2401 gcagcgatta ccgagataat ttttaactcc cggtacttaa gttgtaattt tgtcatcgca
     2461 acagcccaat tatatataat tttatatatt actatataaa tatgaatctt gcaccggaga
     2521 aattgtaagc attattatgc cgactctttt ttatcttatt ttaaaatgga ataccggaca
     2581 tgttaattaa tcgcaatatt gtggcgttat ttgcgttgcc ttttatggca agcgcaactg
     2641 cttctgaatt atccattggt gctggtgcag cttataatga atcgccttat cgcggttata
     2701 atgaaaatac aaaggcaatt ccgctgatta gttatgaagg tgattctttt tatgttcgtc
     2761 agaccacatt aggttttatt ctgtcgcaaa gtgaaaaaaa tgaacttagc ctgaccgcat
     2821 cctggatgcc gctggaattt gaccctgccg ataatgacga tcatgccatg caacaacttg
     2881 ataagcgtga tagtacggct atggcggggg ttgcctggta tcaccacgag cgttggggaa
     2941 ccgtgaaagc ctctgcggct gcggacgttc tggataacag caacggctgg gtgggggagc
     3001 tatcggtatt ccacaaaatg cagataggtc gcctgtcgct gacacctgcg ctgggcgttc
     3061 tctattatga cgagaatttc agtgactatt actatggcat ttcagagagt gagtcccgtc
     3121 gtagcggtct ggccagttat tccgcgcagg atgcctgggt gccctatgtc agcctgacgg
     3181 caaaataccc gataggagag cacgtcgtat tgatggcgag cgcaggatac agcgagctgc
     3241 cggaagagat taccgacagc ccgatgattg atcgtaatga gagttttacc tttgtcaccg
     3301 gcgtgagctg gcgtttttaa ttcaccggtg gatgtcggtg cggctggcct acgctctcgc
     3361 accgactcgt atgcctgata agacgtgtta agcgtcgcat caggcaatgt gctccctacg
     3421 ttggatgcgg cgcgagcgcc ttatccaacc tccggatggc gatgcttttc ctcattcacc
     3481 cagatccgca tttcgccttc gccccggtta gcccagctaa accacgggat aaacgtcaga
     3541 gtttgcggct ggcgtttggc tggcgcgctg tcgtaatgcc acagcggttg ctgctctgga
     3601 ttgctctgtt cataccggta acccggtgcc tggattaaga tcttatggct aaacaatccc
     3661 ttgccttcaa atgtcgtaaa tggcgcatcg gtgggcagcc acagattatg cagtgactcg
     3721 ccgttgtcgg cctgttccag gcaatacacc agcgggccgc gctgaatcgc cacttttccg
     3781 gcgacgtgac gcaccagcgg gttaccgtaa acgcggcgta ccggcatcgg caaagtcaga
     3841 ttcagcgtat cgccctcctg ccattcgcgg gtaatgtgca aatacccttt acgaatatcc
     3901 tgctcgacct cttccccatt caatatgatc tgcggctgtg tgcaccagtc cggcagacgt
     3961 aaagccagcg tatgacgcac cggctggggc gattcgaccg caattgtcac ctgctcctgc
     4021 cacggatagt tcccgctaac ccgcaggcgc agtgtgccat tttctaccgg cacttccatg
     4081 ctgtttcctg cgtatatgtt gatatacaac gcatcttcac gcggcgtgta gagataatga
     4141 ccaatcgagg tcagcacgcg ggcgatattt ggcggacaac aagcgcagcc aaaccaacgc
     4201 tggcggatcg gtttaacgtg atcgtagata tggttgaatt tcagcgattt tggatgtact
     4261 tccagcggat tcacatagaa gaaatgtttg ccgtccagcg ccatgccgcc gagcacggtg
     4321 ttgtacagtg cgcgctccat cacatcggca tattgactgt cgccttccat ttccagcatt
     4381 cgtcgggcaa acatcatcag gccgatggag gcgcaacttt cagcgtatac cgtgtcattc
     4441 ggcagatcgt aatcgctgct gaacgcttca ccgctgctct gtgagccaat gccgccggta
     4501 atatataact gacgctgggc catattgttc cacagccgca ggcagtcctg acgcttgctg
     4561 tcatcgtgac ttaaacgcgc gagatgcgcg acgccggtca tcagatagac aaaacgtacc
     4621 gcgtgaccga tggcggtttg ctgttgcgca aggggcaaat gtgcctggct gtaggctttg
     4681 tctttcacca tccacgccgg gccgtaggtg tgccagtgcg aggtctgccc gcgcttttca
     4741 tattcctggt cgtaatagtg cggttgcaca ccacgctgtt cgacaaaata gttcgtcagc
     4801 gccaggtagc gcggctcttc ggtgacttcg tagagacgca tcagcgccag ttcaatttcc
     4861 ggatgaccgg gataaccctg cagcttgtct tcatctggac caaatacgcg gtcgatatga
     4921 tcggccagac ggcaaaccac ctccagcaag cgtcgttttc ccgtggcctg gaagaaggcg
     4981 actccggctt caatcagatg accggcgcag taaagttcat gacactccgc aagattgctc
     5041 cagcgttctt cgggtgcttt taccgtaaag taagtattga gatagccgtc ttcgcattgg
     5101 gcggaggcga tcagttcgat tacctcgtca gcggtttttt ccagttcggc atcaggcttc
     5161 tggcacagcg accaggctac cgcttccagc catttggcga cgtcgctgtc ctgaaacacc
     5221 atcccgtaaa attcaccctc ctgaagtccg gcggcaatgc gaaagttttc aatcgcatgg
     5281 ctgggttccg cttctgggat acgatcgttc aaggcatccc attgataagg aatcaccacg
     5341 tcgcggacca gttgttggta ctgaccgagg aacggatcgc tgaccgtcag tttatgcaga
     5401 tcgacttccg aaatgttcat cttgtgctcc ttaaggctgt acatgacgct gacgcaggtc
     5461 ggtagctatg cgtgacatca taggattatt gagtttgcaa aagcggatcg cccctgccag
     5521 cagcaggtgg aatagcgcag gcagtaatgt ttccatcgcc gcgatgccgc caagcgaact
     5581 ggcggtctgg ttttccacgc caggctggta ggcgacaaaa ataaacagca ggctgatgat
     5641 cccggcgctg gaagcccagg ccagcttgat gaaaaacaga ttgaaggcga agttcatgcc
     5701 ggaagaacgt acgcgggttt tccactcgcc gtagtcatcg gcaaaggcca tcaatgagaa
     5761 gtgcaatggc aatgtgaagc caagaatcac gccattaccg aggatcaccg ccagccacag
     5821 cgtttgataa gccgggccgg agggcaggaa ccacatcaac accgccagtg cagcgaggag
     5881 caggttggtg tagtaataaa ttttgacggt atcgaaacgc cgcgttaacg ggctgacaat
     5941 caccgagccg ataatggagg cgaaggtgac catggtgaag aacagcgacg tgtagcccgt
     6001 actgccttgt aagacgtagg taatgaagta catataccca ccgccgcgaa tattaaagac
     6061 gttaatcagc aggaaagaca tgaccagcat cagcagcagt tggtcgttgt tccgcagccc
     6121 ggcaagatgt tcgcgcaggg taaatttccc cattgtggag agcggcaccc gttcacgaac
     6181 ccagaaaaaa cagcacagga acatcaccac ggcaatggcg cacaatacgc cgacgcccag
     6241 ttgatagccg cgtgcagcgt tgccctgacc gaagagatct accatccacg gtaagcctac
     6301 cgaaaccaga aatcccgcta cgccgcagag aacgaatcgc caggactggc aggagatcac
     6361 ttcattgtgg cgggtggtca tggtgttgat cagcgcgcag tacggtacgt tgatggcggt
     6421 ataaccgact gaaaggagca ggtaagtacc gaacgcccag gcgattttta ctcccatgct
     6481 ggcatctggc acggtgaacg tcaggatgcc gataatgccg atgggaatgg caatccatag
     6541 ctgccacgga cgaaagcgcc cccatcggct ttgcgtccgg tcggcaatga cgcccatcac
     6601 cgggtcggag atggcatcaa acacgcgcag tgcaataaac agcgtgccaa ccagcgccgg
     6661 agttaatcca aaaacgtctg tgtagaaaaa ggtgagaaaa ttcatgatca ggcaagttat
     6721 tacggtgccg cctgcgtcac ccaggccgta gcctattttt tcccgtacag ataacgcatc
     6781 gtcaatttta ccgttagcga tatcagcggt agtaatcggt gtagaggtca tagatattcc
     6841 tcgtgaatag ggagcaattt ttagttttat tgaaatgacc tgattaattc tgccgtgaac
     6901 gggaatatca acaaggcgag aaaagagtaa aaaaaggtga cgattccgac ctgaatttaa
     6961 aagtgtgaaa tggatcaggt gaaattaatg tttttgatta ataatcaaaa ggtaatgtgt
     7021 ttaatcatta atgaataagc gggaaattac atatcaatgc tcgaattatc catgacgtta
     7081 ccgattaaag tgcaaaatgg tggattattt atttcccgcg gcgtagggag gcatcctgca
     7141 aggcggctgg agtcatggga aattatcttt gtcgaaaaag gtcgattaac gattcaggaa
     7201 gaagaacgga tatttctggt cgatgcggga gagagtttat tactctggcc gaatcgacaa
     7261 catgttgggg tggaggattt tcccgccgat ctcaaatttt actggctgca ttttgaggtt
     7321 aaagcgccgg atagcgaccc gcgctggctc actcatctta gcgtgccgca acaaacgaag
     7381 gttgccgatc cgcaggcgct cgtggccctg tttcgccagt tcttaagcga acaggaaaag
     7441 catcaacgca ctccggcgct ggagtttatt ttgctgctga ttttgcagca gctcacccgt
     7501 gacgcgcgtc ttgatgaaca ggccgatggc gcaggcacca cccttgcctg gaaagcgcag
     7561 aaactgatcc gcacgcaata tcatcgcccg ctttctacgg cgcttctggc ccgcgagctg
     7621 tactgcaatg ccgattatct tgggcgcgtc taccggcggg tgtttcatct gacgttaaca
     7681 gaagcaatcc atcgccagcg agtgcgcgaa gccgaaaagc tgttaattag cgaagcgcga
     7741 tcgctcactg aagtggctgc gttgtgcggg tttaatgacg ttggctattt ccggcagatt
     7801 tttcgcaagc acaccggttt aacgccagcg gcgtggaagc gacgttacag caaggaacat
     7861 atcaattcgt agtgccgggg cgatgaagcc ccggtgtgag ggattactgc ccgtaatagg
     7921 cattgtctcc atgcttacgc aggtagtgct tatccagcag ttcgttttgc atcgcaggga
     7981 gctgcggcgc aagctggcgc gagaacagac ccatataggc gcattcttcg agtactacgg
     8041 cgttatgcac ggcatcggcg gcgtttttac cccatgcgaa cgggccgtga gaatgcacca
     8101 gcaccgccgg gatttgtgcc ggactcctgc cacgttcttc gaaggtttca atgatcactt
     8161 cgccggtctg atattcatat tcgccgttaa tctcctctgc ggtcatctgt cgcgtgcagg
     8221 ggatggcacc atagaaataa tcagcgtgcg tggtgcccca ggcggggaga tccagcccgg
     8281 cctgcgacca gatggtggcg tggcgtgagt gggtatgcac aatgccaccg atttcggcgt
     8341 ggcgacggta aagcgccaga tgcgttggcg tgtcggacga gggctttttg ctaccttcca
     8401 ccaccttgcc gctggctatc tcaaccacca ccatatcgtc ggcggtcatc acgtcatact
     8461 cgacgccgga aggtttgatt accatccatt gccgcgtttc gtctaccgcg ctgacattgc
     8521 cccaggtgaa cgtcaccaga tggtgagcgg gaagcgccag attcgccgcc agcacgtcgg
     8581 ctttcagttg ctctaacata taaatccagc ctcctgcata cgcgcttcaa tccaacgccg
     8641 cgcctgaata atctccagca ccggctcttt ggctttttcg gtccacatct caatcaggaa
     8701 agaaccgcga tagttcagtt tatgcagcgt tttgaagatg ccaacgaaat cgacgcagcc
     8761 ttcgccaaac ggcacgtcgc ggaactgtcc gggactttgc tcggtaactg gctgggtgtc
     8821 tttcaggtgg atcgcggcga tacggtcaat gcccagtttc agttcggcgg gaacatcatt
     8881 gccccaggcg ctgaggttgc cgacgtccgg gtagacggtg aaccacggcg aggcgagcat
     8941 ctcgtcccac tttttccatt tactgatgga gttcataaac gcggtgtcca taatctccac
     9001 cgctagcatg acttgtgatg ccgccgcctg ttcgaccgac cacgccagcc cttcagcaaa
     9061 acgttgccgg gtgccttcat cgtggtcttc gtaatagacg tcgtagcccg ccagctggat
     9121 ggtgcgtatg ccgagatcgc gtgccagacg aatcgctttg ctcatgatct cccgtgctcg
     9181 ctggcgtacc gcgtcgtcac ggctaccaaa aggaaagcga cgatgtgcgg acaggcacat
     9241 cgacgggatc ccaacacccg tttcgatcat cgcggcaacc agcgaagtcc tttgtgcggt
     9301 gctccagtca agacgtgaaa gccgttcgtc ggtttcatcc accgacattt cgacaaaatc
     9361 aaaaccgcag ctttttgcca gcaccagccg ctccggccag gagagatctt tcgccagcgc
     9421 cttttcataa atccctaacg gatgattacg cacgcttgcc tccccagata gcgtcgattt
     9481 gcgcatggaa atcgccagcc acctgcgccg gatttgctga gcttgccagt gctcgcccgg
     9541 caataaacgc tttcacgcga atatctttaa acagcggcag gtcagcaggg gtaatcccgc
     9601 cggtaatgga aagctcaagg ccgatatcgg aaagcgcctt catgcgtgcc agatcggctt
     9661 cgccccactg ttgcccgctg gcctgtgcat cacgaccgcg atgataaatc gcctgccgca
     9721 cgccaatacg atgccagtcg cgggcgtcgt ccagcgtcca gttaccgaac agctcgatct
     9781 gaatttcacc accgcagcgt tgcgccatcg cgtggccttt ttccaccgtc gcgagcggtg
     9841 cggcgcagat gatggtcatc cagttagcgc ctgcgccaaa tgcctgttgc gcgagcgttt
     9901 caccagcgtc ggcgaccttc cagtcggcaa cgatgatttt gtcagggcac tgttcgcgca
     9961 aggctttcac cgcgccaagc ccttcgttta aacagagaat ggtgcccgct tcgacaatat
    10021 cgacgctgtc ttttaactgc gtcacgtcgc gctgtgcggc ttcaagtgat gagtggtcga
    10081 gtgccagttg cagaagtggt cggctcataa tatgtgctcc ttgatgcggg cgtgaaagcc
    10141 ctgaagtgcg gcaatgagat gctgatagcg ttggtatttt tgttggtaaa gctgatgggc
    10201 ggtcatatcc ggcagcaggg tgcgtaccgg gtgctgtaag tcacgttgag cttcgctgaa
    10261 atcgcgataa actccggtgc cgacacgggc ggcaagtgcc gcgccaaaac agccggtttc
    10321 ttccacctgc ggcagctcga tacgcagacc gctgacgtcc gccagcattt gcatccagac
    10381 atcggagtgc gccgggccgc cagtgacgcg cagggtgtgc acatcagtaa aacgttcgcg
    10441 catccggttg aggtgggtca tatggctgaa caccacccct tcataaatgg cctgcaacag
    10501 gtgtgcgcgg gtgtgaatgg cctgcatccc gtagaaaccg ctggtcatct cgagtccggc
    10561 gttgctgccg tacaggaacg gcaggaaaaa gagatcgcca ccggccttcg gcaagctggc
    10621 aacggcctgg ttgatctcat caaacgagat ttctccccac tgtgcggtaa atcattcgag
    10681 gttgccggaa gaggtggggc tggcttcgtg aacgataaat tcaccatcgt taacgtagcg
    10741 accatagaca tacggatgcg cttcaccgtc acgtaaaccg cgggttatgc cgctggtcac
    10801 cgcccaggtg cccatcaccg cattaagggt aaattcgtct tcgatcccgg cgcagagtgc
    10861 ggtggaaacc acatcaaaca ggccgccgac aacgggcgta cccgctttca gaccggtcag
    10921 tacggctgtc tgagcggtga tctccccgca gatttcggca gatccgacaa caggcggcag
    10981 ggcgtgattg atttcagcaa tccccagcca gtcggtgagg cacgggtcat attccccaag
    11041 actcatgttg tagagattgg actcggaaat attgctctct tcacagcctt tgacgccggt
    11101 taaacaccag cgcaggtagt cgtgcgtcat catcacgcag ccaatttgcg cgtagcgttc
    11161 cggttcgtgc tctttaagcc aacgtaacag cgataccgga tgcccggttc acaaggtttg
    11221 tcgggtcagc gggtagagtt tttccgggat gccatcttcc tgccagcgac gaacgatttc
    11281 catcgcccgg cgatccgagg ataaaatggc gttaccgagc ggcttgttgt ttttatccag
    11341 caaaaacaag ccttttcctt gtgcggagat accgataccg acaatttgtt ccccgctaac
    11401 gccggaatgg gtaagcagtt cgcgaatgac agccatgcag cattgccaca gttctgccat
    11461 atcgcgctct gcccaacctg gctgcgggct taatgcgcac agcggcaggc gctgcacgcc
    11521 tgcctcccgg ccttcgcggt catacagccc ggctttcagc cagctaccgc cacaatctaa
    11581 ccccagccag tattgcgtca tggtttattg cacctcatcc acggctttca gtagatcagc
    11641 accctgcgga acatctttta caaacatgtc gcgaacctga ttacccagag cgtcgctaaa
    11701 ggctttgcgg tcgagatcgg tgatgacttc cacgcccgct tctttcatgc cgtcgatgat
    11761 tttttgctga tcttcggcaa ccagtttgcg ctggtagtta cccgcttcct gcgcagatga
    11821 aattagcgct tgctggaact ccgggcttaa gccatcaaac ttcgctttgt tgatcaccac
    11881 cagaagcggg gaataggcgt ggtgcgtcag agaaaggtac ttctgcactt cgaaaaattt
    11941 tgctgaccag acgacgttga tcgggtgttc ctgagcgtcg atagtgcggg tttccagccc
    12001 ggtatagact tcggcaaacg gcatcgggat cgggttagcg ccaaagactt tgaatgcggc
    12061 gatattcatc gggctattgt tggtgcgaat tttcagccct ttcaggtcgg cgggggtttt
    12121 aaccggtgcg cgcgagttgg tgacatcgcg ccagccgttt tcccagtagg ccagtacttt
    12181 tagtccttta ccttcaagtg aggctttcag atcatcaccg actttgccgt cgagcgtttt
    12241 atgcgcgtga gcggtatcgc ggaacaggaa agggacatca agcaagttca tcactggtga
    12301 taacccggca aagttattcg agccggacat ttccatatcg atggtgccgc cacgtacgcc
    12361 gctgatcatc gcctgcgcgt taccgagggt gctatccgga aacaatttta atttcagttc
    12421 gcctttggtt ttttcctgta ataattcatt gaattttttc gccgcaatat gttgcgagtc
    12481 ggtttgtgat gtctcataac cgaaacgtaa tgattgtgcc gccagagaag atgtgctgaa
    12541 tgcagccagg ccagcaatga ataatgcgta ggttacagag cgtaatttca taatattttc
    12601 ctttt
//
LOCUS       DAZKIV010000138        12506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZKIV010000138 DAZKIV010000000
VERSION     DAZKIV010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.138"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(313..1122)
                     /locus_tag="ACHOT6_003741"
     CDS             complement(313..1122)
                     /locus_tag="ACHOT6_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="avirulence protein"
                     /protein_id="HFP9948895.1"
                     /translation="MDGASGAWHLERGLWKTETRWDGNDREQLGKEALGSEEPKPGSP
                     FYGSRGLKLSDSAHSAFSMMLCGSEGPFTKEQALSGFELAQTGQVLAGRLKIQERVKF
                     RADNRIDAQRNGTHSTRTPTGMDLSQDIGTIMRDKAGLPVMSGTSGSSSDATLATRYA
                     AEHFGKTWAAPGLSQAEGCKAISDLSHHYFRAEGSSPPQSMATGINKVRYDAGMEEKY
                     VNTLDIFTHSYPEIYAGVALTIAGAGGNDEQAMYNVTQEAARILHEAETKD"
     gene            complement(1882..1947)
                     /locus_tag="ACHOT6_003742"
                     /pseudo
     CDS             complement(1882..1947)
                     /locus_tag="ACHOT6_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2169..3854
                     /gene="btsS"
                     /locus_tag="ACHOT6_003743"
     CDS             2169..3854
                     /gene="btsS"
                     /locus_tag="ACHOT6_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFP9948896.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3851..4570
                     /gene="btsR"
                     /locus_tag="ACHOT6_003744"
                     /gene_synonym="yehT"
     CDS             3851..4570
                     /gene="btsR"
                     /locus_tag="ACHOT6_003744"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFP9948897.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            4617..5087
                     /gene="yehS"
                     /locus_tag="ACHOT6_003745"
     CDS             4617..5087
                     /gene="yehS"
                     /locus_tag="ACHOT6_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFP9948898.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(5128..5589)
                     /gene="yehR"
                     /locus_tag="ACHOT6_003746"
     CDS             complement(5128..5589)
                     /gene="yehR"
                     /locus_tag="ACHOT6_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HFP9948899.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5714..7714)
                     /gene="yehQ"
                     /locus_tag="ACHOT6_003747"
     CDS             complement(5714..7714)
                     /gene="yehQ"
                     /locus_tag="ACHOT6_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFP9948900.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCTPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSNDPFANDEG
                     NACRQYVQQLSQALWLGGISQPLIHYEAAFSRAQQAAERCNWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEFPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLFATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSMVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(7711..8846)
                     /gene="yehP"
                     /locus_tag="ACHOT6_003748"
                     /pseudo
     CDS             complement(7711..8846)
                     /gene="yehP"
                     /locus_tag="ACHOT6_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            complement(8839..11118)
                     /gene="yehM"
                     /locus_tag="ACHOT6_003749"
     CDS             complement(8839..11118)
                     /gene="yehM"
                     /locus_tag="ACHOT6_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
                     /protein_id="HFP9948901.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     ILTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAVLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(11129..12217)
                     /gene="yehL"
                     /locus_tag="ACHOT6_003750"
     CDS             complement(11129..12217)
                     /gene="yehL"
                     /locus_tag="ACHOT6_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HFP9948902.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFTLGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         2987 a         3160 c         3224 g         3135 t
ORIGIN      
        1 tgcttggcag ctttatgccc ttctgattgg ctgtatgttt gatgacaaaa aattagcgca
       61 agaggacaaa aaatcacctt gcgctaatac tctgtctcag gtcactaatc aactctaact
      121 cattgattaa taggaacgcg aaagattgat cgttgcgtaa cactgcttgg cagaagggac
      181 agaacagatt ctccttcagg agaatctgta tacaatcaat gccagttttt tcgcattgat
      241 tataaaattc atcaataatg ccttgaatgt ggaatatatt cttctggggt aggcctcttc
      301 actagaacat gttcaatctt tagtttcagc ttcgtgcaga atacgagcgg cttcttgcgt
      361 aacattatac attgcctgct catcattacc gccagcccca gcaatcgtca atgcaacccc
      421 agcataaatt tctgggtagc tatgtgtaaa tatatccagt gtatttacat atttctcttc
      481 cattcctgca tcataacgaa ccttatttat tcccgttgcc attgattgtg gtggactgga
      541 gccttctgca cgaaaatagt gatgtgacag atccgagata gctttgcatc cttctgcttg
      601 gcttaagcct ggagcggccc atgtcttacc aaaatgctcg gcagcgtaac gtgttgcaag
      661 agtggcgtca gaggaggaac cggatgttcc gctcatgacc ggtagcccag ctttatcacg
      721 cataattgtg ccgatatctt gagaaagatc catgcctgtt ggtgtgcggg ttgagtgtgt
      781 accgttacgc tgagcatcaa tgcggttgtc tgcgcggaat ttaactcgct cctgaatctt
      841 tagccttcca gccagcactt ggccagtttg agcaagctcg aaaccagaga gtgcttgttc
      901 ttttgtgaat ggaccttcag aaccgcacag catcattgag aaggcagaat gcgcactgtc
      961 ggataatttt agtccacgac taccgtaaaa tggtgaacca ggttttggct cctctgagcc
     1021 aagagcctct ttgcctagct gctcgcggtc gtttccatcc cagcgtgttt ccgttttcca
     1081 aaggccacgc tccagatgcc acgctccaga tgccccatcc atgctgtttg tttgaataca
     1141 ggagattctg gattggttag cccctcaacg gtcgaacgca gaatattctc ttctgctttg
     1201 cgatacctca gataattctc tgctcgtgtt tgcagatgct ctaatgattt gtaattattg
     1261 ttggaaagtg tgatttgtag gaatgatctc acagttatcg gcgagtcagc acctggccga
     1321 taaggttgag ttcctatgtg ggcttggcta cacaatccat ccttcagagt attactgtca
     1381 gctgtatata taccgatttg tgaatcgctt acaactggtt tgattctgtt cggcatatca
     1441 cttgacggtt tattatttac gttttcatca tttggcccag ttgtgaggag tccagaaata
     1501 catctttgac ttaattcggg ctttgctgca aacatatctg ctattaatat catcagatgg
     1561 ctgcttaaaa aaagaacaaa tggtagtgtc cgctattgcc agtacacctc attttgctga
     1621 ttttgtcttt agcgatacag ctctcaacac gcataacacc ctcctgtaac tgtggttatg
     1681 tgcaggtgtt atttttagtt gtatagataa ccagtgtcaa ttgctgatat tgtctatggg
     1741 gcatcaatgg ggcatgtatg ggacactttt tataggcgaa atccgtcgaa gttcgtcgac
     1801 atcgaaaacg aattatctgt ccaacccttg aaaatcggcg ctcctggacg atcttcgttg
     1861 atttttaaaa atgttgcgtc acgcgcgtaa cgtgacaggg ttaatatcac aaagcaacgc
     1921 cacttcacca attgtgtaaa gcgccatcgt ctcacccttg ctcgcgaggt cccggtttaa
     1981 ctttagacgc agttttgcga accaggtagt tttgcccgtt ttttgtgcat ctatagggtg
     2041 attttatttt tgccaggcga ttttgagtga tcgtactcac gaattctcat ttttctgcaa
     2101 gagttcaaag aaagttaaac gcaggcaatg tatgttacgc gttttaaagg gaagtgtggt
     2161 ttgcgggtat gtacgatttt aatctggtat tgctgctgct tcagcagatg tgcgtttttt
     2221 tagtcattgc gtggttaatg agtaaaacgc cattattcat accgttaatg caggtcacgg
     2281 ttcgtctgcc gcataaattt ctctgctaca tcgtcttttc catcttctgc atcatgggca
     2341 cctggtttgg gttgcacatt gacgattcta ttgccaatac ccgtgcgata ggcgcggtaa
     2401 tgggcggctt actcggcggt ccggtcgtcg gtgggctggt tggtctgacc ggcggcttac
     2461 atcgatattc gatggggggc atgaccgcgc taagttgcat gatctcaacc atcgttgaag
     2521 gattgctcgg cggcctggta cacagcatcc tgatccgtcg cgggcgcact gataaagtct
     2581 ttaaccccat taccgccggt gccgtcacgt tcgtcgctga aatggtgcaa atgctgatca
     2641 tccttgcgat cgcccgacct tatgaagatg cggtgcgtct ggtgagtaat attgctgcac
     2701 caatgatggt caccaatacc gtcggcgcgg cgctgtttat gcgtatattg ctcgataaac
     2761 gcgcgatgtt tgaaaaatac acttctgctt tttctgccac tgcgctgaaa gtggcagcct
     2821 cgacggaagg cattttgcga caggggttta acgaagtgaa cagcatgaaa gtggctcagg
     2881 tgctgtatca ggagctggat attggtgcag tcgcgattac cgatcgagag aaattgctgg
     2941 cctttaccgg aattggtgac gaccaccatt tacccggcaa accgatttct tcgacttaca
     3001 ccttaaaagc gattgaaact ggtgaagtgg tctacgctga tggcaacgaa gtaccttatc
     3061 gttgctcttt gcatccgcaa tgcaaactgg ggtcgacgct ggtaattccg ttgcgtggcg
     3121 aaaatcagcg cgtgatgggc accatcaaat tgtatgaagc caaaaaccgt ttattcagtt
     3181 caataaaccg cacgctgggc gaggggattg cgcaactgct ttcggcgcag attcttgccg
     3241 ggcaatatga gcggcaaaaa gcgatgctca cccagtcaga aatcaaactg cttcacgccc
     3301 aggtgaatcc tcattttttg tttaatgcgc ttaacaccat taaagcggtg atccgccgcg
     3361 acagcgaaca ggccagccag ctggtgcagt atctttccac ttttttccgc aaaaacttaa
     3421 agcggccttc ggagtttgtt actctcgccg acgaaattga acatgtgaac gcttatctgc
     3481 aaattgaaaa ggcgcgcttc cagtcgcggt tgcaggtcaa cattgctatt ccgcaagaat
     3541 tatcccagca gcaattgccc gcgtttaccc tgcaaccgat agtggaaaac gccattaaac
     3601 atgggacatc acaactgctg gatacagggc gagtggcaat cagcgcccga cgtgaggggc
     3661 aacatttgat gctggagatc gaagacaatg ccggtttgta tcaaccggta accaatgcca
     3721 gtgggctggg gatgaatctg gtggataagc gtttacgtga acggtttggc gatgactatg
     3781 gaataagcgt cgcctgtgag cctgatagtt acacccgaat aacgttacga ctaccatgga
     3841 gggacgaggc atgattaaag tcttaattgt cgatgatgaa ccgttagcac gggaaaacct
     3901 gcgtgtattt ttgcaggagc agagcgatat tgaaatcgtt ggagagtgtt caaacgccgt
     3961 ggaagggatc ggcgcggtgc ataaactgcg cccggatgtg ctgtttctcg atatccagat
     4021 gccgcgcatc agtggtctgg aaatggtggg gatgcttgac ccggaacatc gcccgtatat
     4081 tgtttttctc actgcgtttg acgaatacgc tattaaagcc tttgaagaac atgcctttga
     4141 ttatctgctg aagccaattg atgaagcgcg actggagaaa acgctggcgc gattgcgtca
     4201 ggagcgcagc aagcaggatg tttcgctgtt accggaaaat caacaggcgc tgaaatttat
     4261 cccttgtacg gggcatagtc ggatttattt gctgcaaatg aaagatgtgg catttgtcag
     4321 cagtcggatg agcggtgtct acgttaccag ccacgaaggg aaagagggct ttaccgaatt
     4381 gacattacgt accctggaaa gtcgtacacc actactgcgc tgccatcgtc agtatctggt
     4441 taacctcgcg catttacagg agattcgtct ggaagataac ggccaggccg agttgatttt
     4501 gcgtaatggc ttaaccgtgc cggtcagccg ccgttatctg aaaagcttaa aagaggcgat
     4561 tggcctgtaa aagactgcta aaatggcttt ttgcctcatc aacacctgaa ggcctcatgc
     4621 taagtaacga tattctgcgc agcgtgcgct acattttgaa agccaataat aatgacctgg
     4681 tgcgtattct ggcgctgggt aatgtcgaag ccaccgcgga acagatcgcc gtctggctac
     4741 gtaaagaaga cgaagagggt tttcagcgtt gtccggacat tgttttgtcg tcattcctca
     4801 atggcctgat ttatgaaaaa cgcggcaagg atgagtctgc tccggcactg gagccggaac
     4861 gtcgcattaa taacaacatc gtgctgaaaa aattacgcat cgcgttttcg ctgaaaaccg
     4921 atgacattct ggctatcctc accgaacagc agttccgcgt ttcgatgccg gaaattacag
     4981 cgatgatgcg cgcaccggat cataaaaact tccgcgaatg cggcgatcaa tttttacgtt
     5041 attttctgcg tggactggca gcgcgccagc atgtgaagaa aagctaagac gggtatggcg
     5101 gccatgcgaa acatggccgc cgacagatta tttcacttct ttaaaaccag cggctttcat
     5161 caccagttcc atttgcgcca tagtgatacc ttttttggca tcttcagcag aaacgttgat
     5221 tcctgaaata ccctgcaggg ctttaaaatc cactttttcc atatcgatag tcacgttttc
     5281 ctgcgcgtag gtatcggtat aggttaattt ttcttcaaca cccgcgatgt ttttgtattt
     5341 ggcgcttaac ggctcaagtg tcttggcagc atcttctttg gtggttgcac caatggaggc
     5401 aaattgaatt ttggtttcag aagattgctt aagcaccttg tcacctttgt agacataggt
     5461 aatggcaatt tcagtgccgt tcagattggc gctgaatttc ttcgattctt ctttgtcacc
     5521 gcagccagca agagagaaaa ccagaacaga tgcaacaacg agggaaaaca gcttattgaa
     5581 agccttcatg taaaactcca ttttatttaa tcaaggaact ggtgactctc accaggggct
     5641 atataggata tgcctaatac cgtggcgtga gcagtccgga actggagtag aactcttagt
     5701 aaaaagcact atttcatcct tgttgctgaa gcatggggaa taattgttcg caaagtaaaa
     5761 caccgttatt cattgcttct acccgtgcct cgctttctgt attacgaaat tgtcccaaca
     5821 catgtgccag ccgataaaaa ccgaccgcgg agaggtcatt cgccagcaac tctgcctgac
     5881 taatagcgct ctgttcctga tagcgccagc cgttatggag cagttgaata agtaacgcct
     5941 ggcagcgcat cagcaactga tgagcggtag acggcacagg caaaacgctg gcagaaggta
     6001 gcggtgccac aggcgcagtt tctgcgtcca gcgcccaggc gcgggttttt gtcatcatca
     6061 cccgtggttc cagtgtcaat tgcccctcta caaagctgac aaagccagaa accatactca
     6121 cggggtcgtc tgtttgttgc aaaagcgccg ccatgcgttc aacggcaaaa ggagaacagg
     6181 cagatgccgg aagggataac gtcaggagat tatcttcacc ttcgccgctg attacctgcg
     6241 catccagcgt ctggcggctg ctatcccaac cgagcgaaat acactcagcg accggcagaa
     6301 taaataagtt atcgacctga ttaagaggcc gtatgcaggc ggggggacgc tggcgtaaat
     6361 attcccgcaa agccacaatg cccggctggc gtaacggcgc gctcaacatt tgccaggcat
     6421 caggcgacag cggcacaacg ctgcttaagc ggttgcgggt agcaaacagc agctcgccat
     6481 cggcactgcg ttttgctgct tgtgaaacaa tttgcccgcc cgctagtgcg ccagcctgaa
     6541 aactaaacag ccgacgcgta gctgccggtg agttttcctg ttcacttcgc ggccaactgc
     6601 gcgaaaggtg caaaatactg ccggtgtcgg gatcggtaaa ccagatgcgt aaaccataat
     6661 gctcaatatc ctgccagcaa cgcataccta aagacaccag ccgcagatga tcaagctttg
     6721 cttctccggc aatgccagag ccaacgaccg tgcgccacgg cacaggagga aattcaccaa
     6781 tactgtcgcg ccgggccatc tcttgtgcgc aatttaatcg actgtttaat gccgcaagct
     6841 gacgtaagca ttctccggca tgatagtggc tggcgcgggc gtggaaggca tcaacgcttg
     6901 cccgtagctg tcgtagtgat tcactcaccc atcgccagtt gcagcgttcc gccgcctgct
     6961 gcgcgcgact aaaagcggcc tcgtagtgaa taagcggctg gctgatgccg cccagccata
     7021 atgcctggct taattgctga acatattgac gacacgcgtt accctcgtcg ttggcaaacg
     7081 gatcgttaga tgatgtgacg tgttcgctgc gcatctgcca gattaaatga gtcaattccg
     7141 cttgctgcgc ttttgcctgg acaaaggcct gtactgccag tacgacatgt tcgcaaagtg
     7201 tgccttcaat acaatcacaa cgggcgaaac gaatgctgct acgggaataa aaacgcacat
     7261 cgctcatcgg taagcgggca gagggaattt cgcccggcgt acagaacaac tcaatggtga
     7321 tgcctttagc gaccagcgcc tgtgcgcgtt tgcgggtagc atcgggaagg gtagccagtt
     7381 cttccagcca gattgccgga tcccactctt cttctttttc cgtagactga gtggtggcac
     7441 aaagtcgttg ataacttaac accagcatca cgcgatgacg gcacataccg ttggccccgc
     7501 aactgcactg agcctctttc agtgcctggc cgttcgccag ctgggtacgg acaccgtcac
     7561 tgaaggtggc gattaaagcg tcgttctcat ggctgatctc cgggacgttg ccattttcca
     7621 gttccttaag actgcgctta acaaaaccgg cattgcttaa cgccgtcagg gcctgcggtg
     7681 tcagttctaa taattccgga cgtagtgaat tcatgactga agattctccg caagccatga
     7741 tgccagctcg cccggcgtca tggcggctat ttgtgcgccg acattaacca gcgcctgggc
     7801 cgtatcgcgg tcatagcaag gtgttgcggt gctatcgagc gctgccagtc ccagcacttt
     7861 gatgccgctc tggacacact ttttcacctg atgcgtcagt aatgatgatg aacccccttc
     7921 gtaaaaatcg ctcacgagga taatgacgct tttcgctggt tgttcaataa gttgccgacc
     7981 atactccacg gcactggcga tattggtccc gccgcccaac tgtactttca ttaataactc
     8041 taccggatcg gcaacgtctg ccgtgagatc aacgacgctt gtgtcaaacg ccaccagatg
     8101 ggtacgaatg ccgggtaact gccacaaaca ggccgccatc accgcagagt ggatcaccga
     8161 atcgaccatc gatccgcttt gatcaaccag taagaccagt tgccattgtt cgctttggcg
     8221 tttaatgcgg ctgttaaagc gggggattcg atatacaact tgccgtgttg cgggtgccag
     8281 tgttgcaggt tggcgcgcag agtacttttg aaatcaaagt ttcgcgccag tggaataaat
     8341 gaacggcgac ggcgatcgcg gacaccagaa aaagcctggc gaacttcctt tgccagtcgc
     8401 gccataattt cttcaacaac ctggcgcact atctggcggg cggcagccag tacttcggga
     8461 ttcatcagat gtttggtgtg caaaacggcg cgtagcaggc tttcggaagg ctgcatacgt
     8521 tccagcacgt cgagatttgt caccacatct tcaatgccgt agcgcagtac ggcatcgctt
     8581 tccagccgct caatcacctg ttgcggaaac agcgtgtgaa tactgttgat ccactcaggg
     8641 gtggtgagat ttgagccacc taatccaccg gagcgttcac cacgctggag ccgttcagga
     8701 tcgcgcccat acagccactc cagcgcgtgg tctatctgcc gggcgttgtc atccagccca
     8761 caaagcgtcg tttctgccgc ttcgccaaga attaatcgcc agcgttgtag ctcacgggtg
     8821 gtcagaagat cgttcagttc agacatggaa aactccccag tgttgcagcg atgccagtgc
     8881 ctgctgttcg agttgttgat gatgtgcaat ggcttgcggt ggacaatgca acggcatttg
     8941 cagtgccgaa acgggaagtt gcgccagttg ataatgctca agcacctgat gcgccagcgt
     9001 cccgcgttct cgtggcggta gccaggccat tgccgcgcgt aaatcgggca gggcattggt
     9061 aaaatcggca tcacttagtt gatttaaatg actgctgaaa ccggcgataa atgccggctg
     9121 acaggccagt tgatgacggg ctaatgccag caaaccgtgc agcgcctcgc cggattgtgc
     9181 cggggataac tgcgccagca tcgtcagcgc cgcttcggca ctggcgttgg gatgttccag
     9241 acgcattagt gcgccaagaa ccgcgccgcg atccagcgcc ggggcatgaa tcgcctgact
     9301 gcgtcgctcc agtaaggcta ccgccgcaga aagcgaaacg ccggataaat taacgccgct
     9361 atgtagatcg cgcagaatat ggcaaagcgc ttgccagcta tgcaggtgag cgtgaaactc
     9421 cttttcatcc ggtctgccgt tagattcaca cagccacagc gtgcgatcga tagccgcgca
     9481 tagcgtcgtt tgtaatatct gcgcgccttg cataccgcta atttcatcca gccgccataa
     9541 ggcatatagc acttccagcg cctggcccat ttcggcaaat tgattttcct gggcgattaa
     9601 taatgtgagt tgctccagta attgttgact gaaggacgcc agacccgcta acgccgcttg
     9661 gctaagacat gtggtgatac tgccgattcc acccgcgtcc agcatatcgg cttctaattt
     9721 atggcgtgcg gcttcctgga gtgtggcacc aaagcaggcg gcctcaatca atgccgcatg
     9781 ctggctaagc gggcgggtta atttccagcg ttcttcaccg ttgcctgcaa gtgtcagtgt
     9841 gcttccctgc tggcgtacaa tccctgggat ctccagtatt gccagccgat gtaacacctg
     9901 actttgcgct agtccattgg ggttaaagcg atttaaggta agctccgccg gaagagagat
     9961 tgcggtacgt tccagttcgc aggtgacatc ttttggcaga ggcggttgtg gcgtagaagg
    10021 ggcaagttta ccgaatccgt cacccgctaa tgtgtctatc aacgttagca gaatcggatc
    10081 ggtatcggga tgaataacgc cgcgatagct ccacggcaac ggcgcattca gggcttcttt
    10141 aatcagcgag cctgctatgg catccagcca gtcagtgcgt aacggtagtg tatgaccgcg
    10201 caactgtgcc agcgccatcg catgcagatg agcggcagcc atatccgcgg tcgaagcagg
    10261 caacttgtgc tggcgcaaac gggtaaggat agtttttagc agttgttcac cggcctgctg
    10321 taagccccac tgccagcacc agttttgcca gaccggggca ggcataccgg aaaggtatcc
    10381 tgccagcaca tcaaggcgct tttcactgta gggcgtgaga taacaacctg taatggcatc
    10441 tgccagcgag ggcaattccg gcgtgttaat gtcctgcggg cattcgcgcc acatttttgc
    10501 cagtgccgga gcgtgccagc caccgcagac gactaacacg tcgccattat tttgctgcac
    10561 cgcccatgca atccagcgag ccataaaggc ttcgcgctga cgattgagtg catcgccggg
    10621 gaaatcgccc cgcagttggg caaaatagtg cgccagcgca gagggtaatg cagtttgctg
    10681 gctttcatct tcgaacaagt gatcccacag ggtatcgctg ttatccatgc gggtggcacg
    10741 cagcagtaag gcctggcttt catcttgcgt atcaggcgaa tcgtcctctt cttcactctg
    10801 cgcccagcaa ggcaaatcga tgaagtaagt ttgtgcctga atacgacgtg cggcttgtag
    10861 cgcctgccac tccggcgaaa attcagcaaa tggcgtccag gcaccacgcc cgggggctgc
    10921 accgtcctga tactggcaat aactgtaaat agctaccgga agctggtggg aaaaaaaaag
    10981 ttcgtctacc cggtcattaa aatcagccgg gccttcaatc aacacgtatc gtggccgctg
    11041 gctttcgatt aacgatttca ccagccgggc gcaggccgga ctatgatgcc ggatgccgac
    11101 aattaacggc tcgctcatgc ctctttcctc acggcaggcg gtggcgggct tgataataag
    11161 cctgccagtg agcttcttta tgtgtggcga cacgctgttc gaagtaacgg cgcagacgag
    11221 cgcgatcttc ttcgttatct ttgacgatgg ttccggcaat acagtccacc aggtcggcag
    11281 gctcccccgc gcgattcgct aaaaaccagg cgcggacgcc tacggcgtgg gccacgttca
    11341 cggcttcagc ggtggacata atcgctgtca gtgtatccat tgacgttttc ttttcgccgt
    11401 tggcacgcag atcgcggaag gtgcgtacca gtaactccag tactgcgtca ggcactttat
    11461 gcggaatacc gctatgggcc agcaaacgcg ccgaagcact ggcgaccagt tccagctcct
    11521 gggcgaaatc cataatcgga aagacggttt caaaatcgaa gcgacgcttt agcgcggcac
    11581 tcatctcatt gacgccgcga tcgcgggtat tggcagtggc aataatatta aagccttccc
    11641 gcgcatagag ctgactggct tcaccggtga gttccggcac cgtcatcacc ctgtcagaaa
    11701 gcatacctaa cagacagtct tgtacttcca gcggtgtacg cgttatctct tcaaaacgga
    11761 cgattttgcc gtcgcgcatt ccctggtaaa gcggggcagg gaccagtgct tccgttgacg
    11821 gaccatggtt gataagcagg gcgtagttcc agccgtactt gatttgatct tcggtagtag
    11881 atgcgccgcc ctggatggtt aacccggcat cgccgctaat agcggttgcc agtaattcag
    11941 acaggagaga ttttgcggtg ccaggttccc ccaccaacat caaaccgcgc ccggtggcaa
    12001 gagtgactaa catgcgttcg acagcggagg gactaatcac cactttacgg ctaatatttt
    12061 gcgcgctatc gccaagggta aaagcacggg ccgcaggcaa acttaactgc caaccgggcg
    12121 ggcaaggtgc gttatcattt tgttttaatt ttgccagttc atcggcgtat aacacagcag
    12181 caggcggacg ctgcagatga ttattctgcg gtgacatata ttcatccctg aaatgtatta
    12241 atgaaataaa cgatatttta cgcgcaataa atgtttggcg cattagttgt ttgagcggaa
    12301 aatatctgga aagcagatca cagtaaataa aaaaacctgc tggtgaggca ggttctttta
    12361 tttactattt tcaggcccaa caccgcagcc tgtgcaacat cgtgttccaa cgaacgaatt
    12421 ataaccacgg ctggaccccg ttgcaattaa gataatgaga tttataatcc aggccattgg
    12481 gacgttgcgc cggatcggac caattt
//
LOCUS       DAZKIV010000139        12452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZKIV010000139 DAZKIV010000000
VERSION     DAZKIV010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.139"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..169
                     /locus_tag="ACHOT6_003751"
     CDS             <1..169
                     /locus_tag="ACHOT6_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFP9948903.1"
                     /translation="QSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRPLK
                     QEPTEMIQATA"
     gene            complement(209..1423)
                     /gene="ydcO"
                     /locus_tag="ACHOT6_003752"
     CDS             complement(209..1423)
                     /gene="ydcO"
                     /locus_tag="ACHOT6_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFP9948904.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLIAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTISGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            1476..2012
                     /gene="sutR"
                     /locus_tag="ACHOT6_003753"
     CDS             1476..2012
                     /gene="sutR"
                     /locus_tag="ACHOT6_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFP9948905.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            2085..4046
                     /gene="rlhA"
                     /locus_tag="ACHOT6_003754"
     CDS             2085..4046
                     /gene="rlhA"
                     /locus_tag="ACHOT6_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFP9948906.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVLIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLLGSVVASVSPDELLKTLPKRKG"
     gene            complement(4138..4368)
                     /gene="yncJ"
                     /locus_tag="ACHOT6_003755"
     CDS             complement(4138..4368)
                     /gene="yncJ"
                     /locus_tag="ACHOT6_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFP9948907.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            4590..4766
                     /gene="hicA"
                     /locus_tag="ACHOT6_003756"
     CDS             4590..4766
                     /gene="hicA"
                     /locus_tag="ACHOT6_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HFP9948908.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            4812..5228
                     /gene="hicB"
                     /locus_tag="ACHOT6_003757"
     CDS             4812..5228
                     /gene="hicB"
                     /locus_tag="ACHOT6_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFP9948909.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            5307..6713
                     /gene="ydcR"
                     /locus_tag="ACHOT6_003758"
     CDS             5307..6713
                     /gene="ydcR"
                     /locus_tag="ACHOT6_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFP9948910.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            6958..8103
                     /gene="ydcS"
                     /locus_tag="ACHOT6_003759"
     CDS             6958..8103
                     /gene="ydcS"
                     /locus_tag="ACHOT6_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFP9948911.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            8121..9134
                     /gene="ydcT"
                     /locus_tag="ACHOT6_003760"
     CDS             8121..9134
                     /gene="ydcT"
                     /locus_tag="ACHOT6_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9948912.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            9135..10076
                     /gene="ydcU"
                     /locus_tag="ACHOT6_003761"
     CDS             9135..10076
                     /gene="ydcU"
                     /locus_tag="ACHOT6_003761"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9948913.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQNFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            10066..10860
                     /gene="ydcV"
                     /locus_tag="ACHOT6_003762"
     CDS             10066..10860
                     /gene="ydcV"
                     /locus_tag="ACHOT6_003762"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFP9948914.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            10882..12306
                     /gene="patD"
                     /locus_tag="ACHOT6_003763"
     CDS             10882..12306
                     /gene="patD"
                     /locus_tag="ACHOT6_003763"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFP9948915.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3010 a         3052 c         3334 g         3056 t
ORIGIN      
        1 acaaagtaaa ttcagatgcc aggtatgtgg atatacagcg aacgccgatg taaatggcgc
       61 tcgtaacatt ttagcggcgg ggcacgccgt tcttgcctgt ggagagatgg tgcagtcagg
      121 ccgcccgttg aagcaggaac ccaccgaaat gattcaggcg acagcctgaa cgtagcaggg
      181 atccacgtcc ttcagggcgt ggaggatgtc aatatcggtt tctgtcagcg attaaattca
      241 acaccacgta acaaacgcct ccggcaatta atccccagaa cgcagaacca atcccgacca
      301 gcgtcaatcc acttgccgtt accagaaatg ccaccaccgc cgcgtctcgc tcacgctcat
      361 tatgcagcgc ctgatacaaa ctgccgctaa tggtacttaa cagcgccaga cctgccagca
      421 tctggatcca acttacgggc agggcagcca tcatcccggt aatagcacta ccaaacagac
      481 ctgcgatcaa ataaaaaatg cctgcaacgg cagctgccag ccaacgttga tctttatccg
      541 gatgcgcttc cgggctttgg caaatagccg cggtgattgc cgcaataccg acggaataaa
      601 cgccgaaagg ggaaaaaacc agtgccagca atccagtaaa tacaattaat ggcgaaacag
      661 gagccgaata cccggctgct ttcattgctg cgatacccgg tgcgttttgc gatgccatcg
      721 tcaccagaaa aagggggagt gcaacgctca ggctgtgagc aaacgaaaaa tcaggggtaa
      781 tataagtggg gagaacgggt ttaaagacaa catcagttgt gacaacgtca ccttgcgcga
      841 taacgatcac gatcccaata atcatcgcgg caattaccgc atagcgcggc gcaacggcct
      901 tggttgccag ccataccagc aacatacttc cacacaacgt aaattgaccg tccagactgg
      961 caaacgcctg taaaccaaag cgtaataaaa tcccggcaag cattgccgcc gcaagcgagt
     1021 gcggaataat gcgcatcaga cgagcaaaga gtcccgttat gccgcagagg actattaacg
     1081 cgttggtgac aataaaaacg ccgatggctt cgttaagtgt tagtccctgc aatccggtga
     1141 ccaacaaagc cgcgccaggc gttgaccatg cggtgagaac aggtacgcga taccataatg
     1201 tcagagtcag cgtactgacg cccattgcca gccccagcgc cgtcatccag ccagagattt
     1261 gtgcagtggt ggctccggcg acaatcgctg cttgccagat tattgccgct gaactggcgt
     1321 agccaattaa taccgccaga aaccccgcca gtagcgtggg tggaggaata gaaaacagac
     1381 gcatagtcac ctcgtgcgtt atagcgtaca ggcaccgtag catttgtccg ttataacgca
     1441 caagtgataa acttccgttt tgccggagga gtcgcatgga aaatctcgct cgctttttat
     1501 ccaccacact taaacaacta cgccagcagc gcggctggag tctttcgcga ctggcagaag
     1561 cgacgggcgt ttctaaagca atgttagggc agattgagcg taatgagtcc agtccgacgg
     1621 tcgcgacatt atggaaaatt gccaccgggt tgaacgtacc attttcaaca tttatttctc
     1681 cgccgcagtc cgctacgcct tcagtttatg atccacaaca gcaggcgatg gtgatcactt
     1741 cgctgtttcc ttatgatcca caactctgct ttgaacactt ctcgattcag atggcatctg
     1801 gtgcaataag cgaatcgacg ccccatgaga agggggtaat cgaacatgtc gtcgtcattg
     1861 atggacaact tgatctgtgc gttgatgggg aatggcagac ccttaattgt ggggaaggcg
     1921 ttcgatttgc tgcagacgtc acgcatatct atcgtaatgg tggggagcaa accgtacatt
     1981 ttcattccct catccattac ccgcgcagtt aagcgggaaa actatttcgc aacgtcgcgc
     2041 ttgtggctaa aatagccgcc atttttcagc tactggataa gaatgtgacc gtatcttctc
     2101 atcgacttga actgttaagc ccggcacgcg atgccgccat tgcccgcgaa gctattttgc
     2161 acggtgccga tgctgtttat atcggcggcc ctggttttgg tgcccgtcat aatgccagta
     2221 atagcctgaa agatattgcc gagctggtgc cgtttgccca tcgttatggt gcaaaaattt
     2281 tcgtcacgct taacaccatt ttgcatgatg atgagctgga acccgcgcaa cggctgatta
     2341 ctgacctcta ccagaccggt gtcgatgcgc tgattgttca ggatatgggg attctggaac
     2401 ttgatattcc gccgattgaa ctgcacgcca gtacgcagtg cgacattcgt acagttgaaa
     2461 aagcgaagtt cctctctgat gttggcttca cgcagattgt gctggcgcga gagctgaatc
     2521 ttgatcagat ccgcgcgatt caccaggcta cggacgcgac cattgaattc tttattcatg
     2581 gggcactgtg cgtggcctat tcgggtcagt gctacatttc tcatgcgcaa acagggcgta
     2641 gcgccaaccg tggcgattgc tcgcaggcgt gccgtttgcc atacacattg aaagacgatc
     2701 aggggcgggt ggtttcctat gaaaaacatc tgctgtcgat gaaagataac gatcagactg
     2761 ccaacctcgg cgcgctgatt gatgctggtg tacgctcctt caagattgaa gggcgttaca
     2821 aagatatgag ctacgtgaag aatatcaccg cccattatcg ccagatgctt gatgccatta
     2881 ttgaagaacg tggcgatctg gcgcgcgctt catcaggtcg tactgaacat ttctttgttc
     2941 catcgacgga aaagactttc caccgtggta gcacagatta ttttgtgaat gcccgtaaag
     3001 gcgatattgg cgcgttcgat tcgccgaaat ttatcggcct gccggtaggc gaagtattga
     3061 aagtggcgaa agatcatctc gatgttgccg ttaccgagcc actggcaaat ggcgatggcc
     3121 tgaacgtgtt gattaaacgt gaagtcgtcg gttttcgtgc caatacggtc gagaaaaccg
     3181 gagaaaatca gtaccgcgtc tggcccaatg aaatgccagc agatttgcac aaaattcgtc
     3241 cacatcaccc actaaaccgt aatcttgatc ataactggca gcaggcactg acaaaaacct
     3301 ccagcgaacg tcgggtggcg gtagacattg aactgggcgg ctggcaggaa caactgattc
     3361 tgaccctcac cagtgaagag ggtgtcagca tcacgcatac gctggacggg cagttcgacg
     3421 aagccaataa cgccgaaaaa gcaatgaaca atctgaagga tggtctggca aaactggggc
     3481 aaaccctcta ttacgcccgc gatgtgcaaa ttaatttgcc gggggcgctg tttgtaccaa
     3541 acagtctgtt aaaccagttc cgccgtgaag ctgctgacat gctggatgct gcgcgtcttg
     3601 ccagttacca gcgcggcagc cgtaaaccgg ttgctgatcc tgcgccggtt tatccgcaaa
     3661 cgcatctgag tttcctcgcg aacgtataca accagaaagc gcgtgaattt tatcatcgct
     3721 atggtgtgca gctgattgac gcggcgtatg aagcacatga agagaagggc gaagtcccgg
     3781 tgatgatcac caagcattgt ctgcgctttg cctttaatct gtgcccgaaa caggcgaaag
     3841 gcaatatcaa aagctggaag gcgacgccaa tgcaactggt taacggcgat gaagtattaa
     3901 cgctaaagtt tgattgccgc ccatgcgaga tgcacgtcat tggcaaaatc aaaaatcaca
     3961 tactgaaaat gccgttactg ggaagcgtag tggcatccgt aagtccggat gagctgctga
     4021 aaacattgcc taagcgaaaa gggtaaaacg ccagttttct ggttactcac aactcattga
     4081 atctgcatga tattgcctgc cgggtaaggc gtcacgccgc atccggcaac aaatgactca
     4141 acgttgtccc gcttctggtt tgcgcgattt ttgccagtaa aaatgctcgc gcaaaccttc
     4201 cgccgactct tccgccacag cacgcaattc atcgctgtcc gcttcatgac gcagctgatg
     4261 atccacattc tttacccaca caaattcatg tcctttgtgc cctgccatga gttgtcctga
     4321 gaacagcgca cacgttaata agacaaccga taacgccttc gtaaacatcc tgccaccttt
     4381 ttcttacctt ttgccgctat gatgccgatc gtttcttgag gttattattc agttttgcaa
     4441 attagcgcaa agaaattctg gaatcttcct tcctgatttt gcatggcatt ctgccgttgc
     4501 ggcgatttag tgctattttc gagcaaatta cacacggagg taaacactaa tgtttacttt
     4561 gttgatatac tcagcggcag ggaggcgatg tgaaacaaag cgagttcaga cgttggctcg
     4621 aatctcaggg cgtcgatgta gcgaatggca gcaaccattt gaaactcagg tttcatggga
     4681 ggcgcagtgt catgccgcgt cacccctgcg atgagattaa agaaccattg cgtaaagcaa
     4741 tcctgaaaca actcggtttg agttaatcgc caattaaaaa ggttaatgac atgcgaggga
     4801 cagtcgaaat tatgcgttat cccgtcactc ttacacccgc gccggaaggc ggttatatgg
     4861 tttcttttgt ggatatccct gaagcgttga cgcagggcga aactgtcgct gaagcgatgg
     4921 aagcggcaaa agatgcttta ctgaccgcat ttgattttta ttttgaagat aacgagctta
     4981 tccctttacc ttcgccatta aatagtcatg atcactttat tgaagtacct ttgagcgtcg
     5041 cctctaaggt attgctgtta aatgcttttt tacagtcaga aatcactcag caagagttag
     5101 ccaggcgaat tggcaaacct aaacaggaga ttactcgcct atttaacttg catcatgcga
     5161 caaaaatcga cgccgtccag ctcgcggcaa aggcgcttgg caaagagtta tcgctggtga
     5221 tggtttaatt acagttaacg aaaagttgtc atttttaaca actgatatag actgccgaat
     5281 catctgcaca taattacgat tcgataatga aaaaatacca gcagcttgca gaacaattac
     5341 gcgagcagat tgcgtcgggt atctggcaac ccggcgatcg tttgccttcg ttgcgtgacc
     5401 aggtggcgct ttcaggcatg agctttatga ctgtcagcca tgcctatcag ttgctcgaaa
     5461 gtcagggata tattatcgca cgaccgcagt cgggttatta cgttgcgcca caggcaataa
     5521 aaatgccgaa agcgccagtc attccagtca ctcgagatga agcagtcgat atcaacactt
     5581 atatttttga tatgttgcag gccagtcgcg atccgtcggt cgttccgttt gcctcggcct
     5641 ttcccgaccc gcgacttttc cccctccaac aactaaaccg ctcgctggcg caggtaagca
     5701 aaaccgccac agcgatgagc gtgattgaaa acttaccgcc aggaaacgca gaactgcgtc
     5761 aggctattgc tcgtcgctat gccttacagg gcatcaccat ttctcctgat gaaattgtca
     5821 ttactgccgg ggcgttagag gcattaaacc tcagtttgca agcggtaact gaaccgggcg
     5881 attgggtgat agtagagaat ccttgtttct acggtgcgtt gcaggcgctg gagcggctac
     5941 ggctgaaggc gttatcggtg gcgacggatg ttaaagaagg gatcgatctt caggcgctgg
     6001 aactggcgtt gcaggattat ccggtgaaag cgtgctggct gatgactaat agccagaacc
     6061 cactcggatt taccttaacg ccgcaaaaaa aagcacaact ggtggcgtta ctcaatcagt
     6121 acaacgtaac gctgattgaa gatgacgttt acagcgaact ttattttgga cgggaaaaac
     6181 cgctgcctgc gaaagcgtgg gatcgccacg atggcgtttt gcattgctct tcgttttcga
     6241 aatgtctggt gcctggtttt cgtattggtt gggtcgccgc cggaaaacat gcacgtaaaa
     6301 ttcaacgctt gcagttgatg agtacgcttt ccaccagctc accgatgcaa cttgcgctgg
     6361 tggattacct ttccacgcgc cgatacgacg cccatcttcg tcgcctgcgt cgccagcttg
     6421 cggaacgtaa acaacgtgcc tggcaggcac tgctgcgtta tctgcctgcg gaagttaaaa
     6481 ttcatcatag tgacagcggt tattttctct ggctggagct ccccgagccg ttagatgccg
     6541 gcgaattaag cctggcggca ctgacgcatc atatcagtat tgcgccgggt aaaatgtttt
     6601 ctaccggtga aaactggtca cgtttttttc gttttaatac cgcgtggcag tggggagaac
     6661 gtgaagaaca ggcggtaaaa caattaggca aacttattca agaacggctg taatagcgtt
     6721 taatttaatt cctcttagat tgggtaatat gaatttcgaa tagcagtcat atttcctaac
     6781 cccttgtcta tactccagaa gataacctta cagacggcat aatgcgcggt agctcacaac
     6841 ctgattaaat tttctcaggg gcgaaggtgt gcctgcaagc cgccgtctat ggttaaacaa
     6901 ggagatattt ttacggcacg gcggctgaac aattaattac gacaggagta agaccttatg
     6961 agcaagacat ttgcccgcag cagcctgtgt gcgctcacca tgacaataat gaccgctcac
     7021 gccgccgaac cacctaccaa tttagataaa ccggaagggc gactggatat tatcgcctgg
     7081 ccgggataca tcgaacgcgg acaaactgat aaacaatacg actgggtaac gcaatttgaa
     7141 aaagagacag gctgcgcggt gaatgtgaaa accgccgcga cttccgatga aatggtcagt
     7201 ctgatgacca aagggggtta cgatctggtt acggcatccg gcgatgcctc gctgcgtttg
     7261 attatgggca aacgcgtgca gccgattaat accgccttga ttcccaactg gaaaacgctc
     7321 gatccgcgcg tggttaaagg cgactggttt aatgttggcg gcaaagttta cggcacacct
     7381 taccaatggg ggccgaacct gctgatgtac aacactaaaa ccttcccgac gccgccggat
     7441 agctggcaag tggtttttgt tgagcaaaat ctgccggacg gcaagagcaa taaaggccgc
     7501 gttcaggctt atgatggccc tatctacatt gcggacgctg cgttgttcgt taaagccact
     7561 cagccgcagt tgggcatcag cgatccgtat caactcaccg aagaacagta ccaggcggtg
     7621 ctgaaagtgc tgcgcgctca acacagtttg atccatcgct actggcatga cactaccgtg
     7681 caaatgagcg atttcaaaaa cgagggtgtg gttgcttcca gtgcctggcc ctatcaggcc
     7741 aacgccctga aagccgaagg ccagcctgtt gctaccgttt tcccgaagga gggtgttacc
     7801 ggttgggctg ataccaccat gctgcatagc gaagcgaaac atccggtttg cgcctacaaa
     7861 tggatgaact ggtcattaac gccaaaagtg cagggcgatg tggcggcctg gtttggctcg
     7921 ttaccggtag tgccggaagg gtgtaaagcc agtccgttat taggcgaaaa aggttgtgaa
     7981 accaacggtt ttaactattt cgacaaaatc gccttctgga aaacgcctat agcagaaggg
     8041 ggcaagtttg ttccctacag tcgctggacg caggattaca ttgccattat gggcggtcgc
     8101 taacttcgct ggggtgcttt atgacgtacg cagtggagtt tgacaacgtc tcgcggttgt
     8161 acggtgacgt gcgcgcagta gatggcgtca gtattgcgat aaaagatggt gagttcttct
     8221 ctatgctggg gccgtccggc tccggcaaaa ccacctgcct gcgcctgatt gctggcttcg
     8281 aacagctttc cggcggggct atctctatct ttggtaaacc cgccagcaat ctgccaccgt
     8341 gggagcggga cgtgaatact gtctttcagg actacgcgct atttccgcat atgtcgattc
     8401 ttgacaatgt cgcctatggg ctgatggtca aaggcgtgaa taaaaagcag cggcacgcaa
     8461 tggcgcaaga ggcgctggag aaagtggcgt tggggtttgt acatcaacgt aaaccgtcac
     8521 aactttctgg tggtcagcgc cagcgggttg ctatcgccag agcattggtg aatgaaccgc
     8581 gcgtattgct gttggatgaa ccgctcggcg cactggatct caaattgcgt gagcagatgc
     8641 agctggaact gaaaaaactg caacagtctc tcggtatcac ttttatcttc gttacccacg
     8701 atcagggcga agcgttatcg atgtccgatc gtgtggcggt tttcaataat ggacgcattg
     8761 agcaggtcga ttccccgcgc gatctctata tgcgcccgcg cacgccgttt gttgccggat
     8821 tcgtcgggac atccaacgtt tttgatggac tgatggcaga gaaactttgt ggcatgacgg
     8881 gaagcttcgc cctgcgaccg gaacatatcc gcctcaacac ccctggtgaa ctgcaggcca
     8941 atggcacgat ccaggcggtg caatatcagg gcgcggcaac tcgttttgaa ctgaaattga
     9001 acggcggtga aaaactgctt gtgagtcagg ccaatatgac aggcgaagaa ctgcctgcca
     9061 cgctcacgcc cggacaacag gtgatggttt cctggtcgcg tgatgtgatg gtgccgctgg
     9121 ttgaggagag gtgaatggcg atgaatgtat tgcaatcacc ttcacgtcca ggtctgggta
     9181 aggtgtccgg tttcttctgg cgtaatccgg ggctggggct gtttttactg ctgcttggcc
     9241 cgctaatgtg gtttggcatt gtctatttcg gctcgctgct gacactgtta tggcagggat
     9301 tttatacttt tgacgatttc accatgtcgg taacgccgga actgacgctg gcgaatatcc
     9361 gtgcgctgtt taatccggcg aattacgaca tcattctccg cacgctgact atggctgtgg
     9421 cagtcactat cgccagcgct attctggctt ttccaatggc gtggtatatg gcgcgctata
     9481 ccagcgggaa aatgaaagct tttttttata ttgcggtaat gttgccgatg tgggcgagct
     9541 acattgttaa agcctatgcc tggacgttat tgctggcaaa agatggcgtg gctcagtggt
     9601 ttttacaaca tcttgggctg gaaccactgc tgactgcgtt ccttacatta cctgcggtgg
     9661 gcggaaatac gctgtcaact tccgggctgg ggcgctttct ggtgtttctc tatatctggt
     9721 tgccgttcat gatcctgccc gttcaggcgg cgcttgagcg tttgccgccg tcattgttgc
     9781 aggcgtcggc tgatctcggc gcacgtccac gacaaaactt tcgctatgtg gtgctgccgc
     9841 tggcaatccc gggtattgcc gctggctcta tctttacctt ctcactcaca ctgggcgatt
     9901 ttatcgtccc gcagctggtt ggtcctccag gatattttat cggcaatatg gtttattccc
     9961 agcagggggc gattggcaat atgccgatgg cggcggcatt caccctggtg ccgattgttc
    10021 tcatcgcact gtacctggcg ttcgtgaaac gtctgggagc gttcgatgca ctctgaacgc
    10081 gcaccgtttt tcctcaaact ggcggcctgg ggcggcgtgg ttttcctaca ttttcccatc
    10141 ctgattatcg ccgcttatgc gtttaacact gaagatgcgg cgtttagttt tccaccgcag
    10201 ggcctgacgc tgcgctggtt tagcgtggca gcacagcgta gtgatattct tgatgccgtg
    10261 acactgtcac ttaaagtggc ggcgctggcg acattaattg cgctggtgtt agggacgctg
    10321 gcagctgccg cgctgtggcg acgagacttt ttcggcaaaa acgccatttc gctgttactg
    10381 ctgctgccca ttgcgctgcc gggcattgtc actggtctgg cgttattaac cgcctttaaa
    10441 accatcaatc tggagccggg atttttcacc atcgtggtcg gtcatgcgac tttttgtgta
    10501 gttgtggtgt ttaacaatgt catcgcccgt tttcgccgca cctcctggag tctggttgag
    10561 gcgtctatgg atcttggggc caatggctgg caaaccttcc gctacgtagt gttgccgaat
    10621 ctcagttcgg cgttactggc aggaggaatg ctggcgtttg ccttgtcgtt cgatgaaatc
    10681 atcgttacga cctttacggc aggtcatgaa cgaacgttac cgttgtggtt gctcaatcag
    10741 cttgggcgac cgcgtgatgt accggtaact aacgtggtgg cactgctggt tatgttggta
    10801 acaaccttgc cgatcctggg ggcctggtgg ctaacccgcg aaggcgacaa tggtcaataa
    10861 ccactgatac aggaatatgc tatgcaacat aagttactga ttaacggaga actggttagc
    10921 ggcgaagggg aaaaacagcc tgtctataat ccggcaacgg gggacgtttt actggaaatt
    10981 gccgaggcat ccgcagagca ggtcgatgct gctgtgcgcg cggcagatgc agcatttgcc
    11041 gaatgggggc aaaccacgcc gaaagtgcgt gcggaatgtc tgctgaaact ggctgatgtt
    11101 atcgaagaaa atggtcaggt ttttgccgaa ctggagtccc gtaattgtgg caaaccgctg
    11161 catagtgcgt tcaatgatga aatcccggcg attgtcgatg tttttcgctt tttcgcgggt
    11221 gcggcgcgct gtctgaatgg tctggcggca ggtgaatatc ttgaaggtca tacttcgatg
    11281 atccgtcgcg atccgttggg ggtcgtggct tctatcgcac cgtggaatta tccgctgatg
    11341 atggccgcgt ggaaacttgc tccggcgctg gcggcaggga actgcgtagt gcttaaacca
    11401 tcagaaatta ccccgctgac cgcgttgaag ttggcagagc tggcgaaaga tatcttcccg
    11461 gcaggcgtga ttaacatact gtttggcaga ggcaaaacgg tgggtgatcc gctgaccggt
    11521 catcccaaag tgcggatggt gtcgctgacg ggctctatcg ccaccggcga gcacatcatc
    11581 agccataccg cgtcgtccat taagcgtact catatggaac ttggtggcaa agcgccagtg
    11641 attgtttttg atgatgcgga tattgaagca gtggtcgaag gtgtacgtac atttggctat
    11701 tacaatgctg gacaggattg tactgcggct tgtcggatct acgcgcaaaa aggcatttac
    11761 gatacgctgg tggaaaaact gggtgctgcg gtggcaacgt taaaatctgg tgcgccggat
    11821 gacgagtcta cggagcttgg acctttaagc tcgctggcgc atctcgaacg cgtcagcaag
    11881 gcagtagaag aggcgaaagc gacagggcac atcaaagtga tcactggcgg tgaaaagcgc
    11941 aagggtaatg gctattacta tgcgccgacg ctgctggctg gcgcattaca ggacgatgcc
    12001 atcgtgcaaa aagaggtatt tggtccagta gtgagtgtta cgcccttcga caacgaagaa
    12061 caggtggtga actgggcgaa tgacagccag tacggacttg catcttcggt atggacgaaa
    12121 gatgtgggca gggcgcatcg cgtcagcgca cggctgcaat atggttgtac ctgggtcaat
    12181 acccatttca tgctggtaag tgaaatgccg cacggtgggc agaaactttc tggttacggc
    12241 aaggatatgt cactttatgg gctggaggat tacaccgtcg tccgccacgt catggttaaa
    12301 cattaagtta gatgttagtg gctgttatag gacggccact atcgttttag gttctgagtt
    12361 taatggcggt taagtattag aatgattaca ttaataacta gcgtaaaaat taggctaaat
    12421 gtttgatggc gatttttcat cctgtcacag tt
//
LOCUS       DAZKIV010000140        12429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZKIV010000140 DAZKIV010000000
VERSION     DAZKIV010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.140"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..1135)
                     /gene="fbaA"
                     /locus_tag="ACHOT6_003764"
     CDS             complement(56..1135)
                     /gene="fbaA"
                     /locus_tag="ACHOT6_003764"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFP9948916.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(1350..2513)
                     /gene="pgk"
                     /locus_tag="ACHOT6_003765"
     CDS             complement(1350..2513)
                     /gene="pgk"
                     /locus_tag="ACHOT6_003765"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFP9948917.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(2563..3582)
                     /gene="epd"
                     /locus_tag="ACHOT6_003766"
     CDS             complement(2563..3582)
                     /gene="epd"
                     /locus_tag="ACHOT6_003766"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFP9948918.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEISVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            3954..4385
                     /locus_tag="ACHOT6_003767"
     CDS             3954..4385
                     /locus_tag="ACHOT6_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFP9948919.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            4408..4986
                     /locus_tag="ACHOT6_003768"
     CDS             4408..4986
                     /locus_tag="ACHOT6_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFP9948920.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            4987..5694
                     /locus_tag="ACHOT6_003769"
     CDS             4987..5694
                     /locus_tag="ACHOT6_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFP9948921.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNNLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            5682..6359
                     /locus_tag="ACHOT6_003770"
     CDS             5682..6359
                     /locus_tag="ACHOT6_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9948922.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            6353..7030
                     /locus_tag="ACHOT6_003771"
     CDS             6353..7030
                     /locus_tag="ACHOT6_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9948923.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKIGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANAHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(7002..7715)
                     /locus_tag="ACHOT6_003772"
     CDS             complement(7002..7715)
                     /locus_tag="ACHOT6_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFP9948924.1"
                     /translation="MKIELTVNGLKVQAQYSDDEIENVHKPLLRMLAALQAVSSQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVVKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(7712..8221)
                     /gene="fumE"
                     /locus_tag="ACHOT6_003773"
     CDS             complement(7712..8221)
                     /gene="fumE"
                     /locus_tag="ACHOT6_003773"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFP9948925.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(8243..9208)
                     /gene="glpX"
                     /locus_tag="ACHOT6_003774"
     CDS             complement(8243..9208)
                     /gene="glpX"
                     /locus_tag="ACHOT6_003774"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFP9948926.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGGRTQTLLIGGADQTCNIIDSLH"
     gene            complement(9205..10482)
                     /locus_tag="ACHOT6_003775"
     CDS             complement(9205..10482)
                     /locus_tag="ACHOT6_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFP9948927.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(10497..11885)
                     /gene="cmtA"
                     /locus_tag="ACHOT6_003776"
     CDS             complement(10497..11885)
                     /gene="cmtA"
                     /locus_tag="ACHOT6_003776"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFP9948928.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPEKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(11913..12356)
                     /gene="cmtB"
                     /locus_tag="ACHOT6_003777"
     CDS             complement(11913..12356)
                     /gene="cmtB"
                     /locus_tag="ACHOT6_003777"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFP9948929.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
BASE COUNT         2936 a         3399 c         3032 g         3062 t
ORIGIN      
        1 tttggcgaaa aaaagacccg cagagcgggc cttgagataa gcagaaagga atatcttaca
       61 gaacgtcgat cgcgttcagt tcctggaatg ctttttccag acgagcgatc atcgaagtct
      121 gaccggcacg cagccatacg cgcggatcgt agtatttctt gttcggctga tcttcgcctt
      181 tcgggttacc caactgcccc tgcagataag cttcgttcgc tttgtagtag ttcagaacgc
      241 cttcccaggt tgcccattgg gtatcggtat cgatgttcat ttttactacg ccgtagctta
      301 cggagtcttt gatttcctga gcagtagaac cggaaccacc gtggaatacg aagttcaggc
      361 tgttgtgcgg caggttgtgt ttcttagaaa catattcctg agaatcacgc aggatggtcg
      421 gagtcagaac cacgttacct ggcttgtaaa caccgtgtac gttaccgaag gacgcagcga
      481 tggtgaaacg cgggctgatt ttgctcagtt cggtgtatgc gtaatcaacg tcttccggct
      541 gggtgtacag tgcagaagcg tccatgtggc tgttgtccac gccgtcttct tcaccaccgg
      601 tgcaacccag ttcgatttcc agagtcatgc cgattttgga catacgctcc aggtatttag
      661 agcagatttc gatgttctct tgcagagatt cttcagacag gtcgatcatg tgagaagaga
      721 acagcggctt accggtagct gcgaagtgtt tttcacccgc gtccaacaga ccgtcgatcc
      781 acggcagcag tttcttcgcg cagtggtcag tgtgcaggat aaccggaaca ccataatgtt
      841 cagccatctg gtgaacgtga tgcgcaccag agatcgcgcc caggatagca gcaccctgcg
      901 gaacgtcaga tttcacgcct ttaccagcga taaaggaagc accaccgttg gagaactgaa
      961 cgataaccgg cgctttaact ttagcagcag tttccagtac ggcgttgatg gagtcagtac
     1021 cgacgcagtt tactgctggc agtgcgaagt tgttttcttt tgctacctgg aaaactttct
     1081 gtacgtcatc accagtgatt acgccaggtt ttacgaaatc aaaaatctta gacatgtctc
     1141 ttgtcctgta tcgtcgggcc ttatactcat catacttcaa gttgcatgtg ctgcgtctgc
     1201 gttcgctcac cccagtcact tacttatgta agctcctggg gattcactct cttgtcgcct
     1261 tcctgcaact cgaattattt agagtatgaa aaaatgcgtg ctctaaaagc gcgctgaaac
     1321 aagggcaggt ttccctgccc tgtgattttt tacttcttag cgcgctcttc gagcatcgct
     1381 actgcaggca gtactttacc ttccacgaat tcgaggaatg cgccgccgcc agtggagatg
     1441 taggagattt tgtcagcaat gccgaacagg tcgattgctg ccagagtgtc gccgccgcca
     1501 gcgatggaga acgcttcgct gtctgcgata gcgttagcca cgatttcagt acctttgcgg
     1561 aagttcggga attcgaacac gccaaccgga ccgttccaca gaatggtttt cgcattcttc
     1621 aggatttcag ccagttcctg agcggaagca tcaccgatat ccaggatctg ctcgtcagct
     1681 ttcacatcgt taacagattt cagggtagcc ggtgcagttt cagagaactc ggttgctacg
     1741 cgaacatcag acggaaccgg gatgttgcag gtggtcagca gacgtttagc ttcgtcaacc
     1801 aggtcagctt cgtacaggga tttacccaca tcgtggcctt gtgccgcgat aaaggtgtta
     1861 gcgataccac caccaacaat cagctggtca gcgattttag acagggagtc cagaacggtc
     1921 agtttggtag atactttaga accaccaacg atagccacca tcgggcgagc aggttctttc
     1981 agtgctttac ccagcgcgtc cagttcagct gccagcagcg ggcctgcgca cgcaacgtca
     2041 gcgaatttac cgataccgtg agtagaagcc tgcgcgcggt gagcagtacc gaatgcgtcc
     2101 attacgaaca cgtcacacag tgcagcgtat tttttggaca gggtttcgtc gtctttcttc
     2161 tcgcctttgt tgaagcgaac gttttccaga acaaccagtt caccttcagc aacgtcaacg
     2221 ccgtcgaggt aatctttaac cagacgaacc gggttagaca gtttgtcttt caggtagtta
     2281 acaaccggca gcagagagaa ttcttcgttg tactcgcctt cggtaggacg acccaggtgg
     2341 gaagttacca tcacttttgc gccttgtttc agggccagtt cgatggtcgg cagagaagca
     2401 cggatacgcg cgtcgctggt tactttcccg tcttttactg gtacgttcag atccgcacgg
     2461 ataaatacac gtttcccagc aagatccaga tcggtcatct taattacaga catggtgaat
     2521 cctctcgttg attctaaaag ttttgcagac gctgcttgcg tcttacctga aagcaacagt
     2581 agccatagct aacgtcgtgt cgagcattcg gttagcaaag ccccattcgt tatcgcacca
     2641 gaccaacgtt ttgatcaggt gtgcgccact gacccgggtt tgggtgccat cgacaatggc
     2701 actgtgcgga tcgtggttaa aatctacaga gaccaacggc aattccgtat agtcaactat
     2761 accatgaaat gcaccttgtg ctgctttttg cagcaacagg ttgacttcat tggcttttac
     2821 aggtttcttc accgtcacgc ttaaatcgat tgccgtcaca tttatggttg gcacacgtac
     2881 cgcaatcgct tcaaagcgat cgttaaattg cggaaaaaat cgtgtgatac cggcggccag
     2941 tttagtatcg accggaatga tcgactggct ggctgcccgg gtgcgacgca ggtcaggatg
     3001 gtatgcatca ataacctgtt gatcgtgcat ggcggagtga attgtggtca cagtgccgga
     3061 ctcaataccg tacgcatcat ctaacaattt gatgacggga attatgcaat tcgtggtaca
     3121 ggaagcgtta gaaacgatgc ggtgttccgc acgaagttga tcctgattga cgccgtaaac
     3181 aacggtcgcg tcgagatcgt tactgccagg atgtgaaaag agcacttttt tggccccggc
     3241 ggcaatatgc gcttcgccat gctcgcggga gccatatacg ccggtgcagt cgaggactac
     3301 atcaacgcca agttcacgcc aggggagcga ttgcagtgaa cgttcatgca atacgcggat
     3361 ggcgtcatca ccaacaaaaa gttgatcgcg ttcctgtcgt acttcccatg caaaacggcc
     3421 atggctggtg tcatatttca acaaatgcgc catgcccgca gcatccgcca gttcgttgat
     3481 tgccaccacg ctaatttccg cccggcgtcc ggattcatac aaagcacgaa ccacattacg
     3541 cccgatgcga ccgaagccat ttatcgctac gcgtacggtc atagatctcc tgcaaggttt
     3601 tccctgagca aatttgccag acagagtaat ccagcaaatc gtccggggaa accttacctg
     3661 tcgcaaactg cgactgattg gttaattgtc gaacatttaa tcgactgaaa cgcttcagct
     3721 aggataagcg aaacgtggaa taaaaggaat gattgtccag tcaaagaaag caattatctg
     3781 acttacgtca cgttttcagc cccgcccctg tggaatttat tccactttgc agaatcacga
     3841 ataagcgtag caaaatcttt tcgttgtgcc gtcacaaacc ctgacttata tcagaatatg
     3901 actgatgcag cgtcgatatt atccgcaata cgtccgagtt tcggagtact ccaatgatga
     3961 cattgcccta cccatattct tcctccgcac gcttttacgc actgcgcctc ctccccgggc
     4021 aggaagtttt ttcccagcta cacgcttttg cacagcagca tcaacttcat gccgcctgga
     4081 ttgcaggctg taccggtagc ctgactgacg tcgccttgcg ctatgccggg caagaaggca
     4141 ccacgctcct gaacggtaca ttcgaagtga tctcactaaa cggcacgctg gaacagagtg
     4201 gtgagcatct tcatctctgc gtttccgatc cacacggcac gatgcttggc ggtcatatga
     4261 tgcccggctg taccgtgcgg accacgcttg aactggtgat cggtagcctt gaggaactgg
     4321 cgtttagccg ccaaccttgc gcgctttctg gttacgacga gttgcatatt tcccccgtta
     4381 aataactact ttcaaagagg ttttcttatg gcgcgtagcc acttttccag tcaggcactg
     4441 gtgttgatcg ttatatctat cgccatcaat atgatcggcg ggcaactggc cagcatggtc
     4501 aaattaccta tatttctcga ctccatcggc acacttatta gcgcggtact gcttggtccg
     4561 gtgatcggaa tgctgaccgg cttactgacc aatttactct ggggattgct gaccgacccg
     4621 atcgccgccg catttgcacc cgtcgcgatg gtcatcggcc tggtcgccgg ttgcctggcg
     4681 cgagcgggtt ggtttcgcac cctgccaaaa gtcgttgtca gcggcgtgat tattacgctt
     4741 gcggtaacgg tcgtcgctgt accgctgcgt actgcgctgt tcggcggtgt caccggtagc
     4801 ggagccgatt tgttcgttgc ctggatgcac tcgatggggc aaaacctggt ggagtcggtg
     4861 gcgattacgg tgataggcgc gaacctggtc gataaaattc ttacagcggt gattgtctgg
     4921 cttttactgc gccagctgcc gattcgcacc acgcgccatt ttccggcaat ggctgccgtg
     4981 cgctaaatgc atccatttac ctcattaaca ctgtgggcat tggcggcctg caccacgctt
     5041 atcctgcccg cgcagaccat tttgccgatc tatagcgcgg cgaccttttt ctgccttatt
     5101 gcgctaaagg ccacgcggcg gcgggcaaaa tatgtcgtct ggctgatgtt ctcgctggga
     5161 gccgggctgt ggctggtaca cggtggctgg ctaccagaat ggctaagcgg cacgccacgc
     5221 tcgccagaac ggtggtcgca tgctattacg ctctggttgc ggattctggc gattgtttca
     5281 acctcacagt tgtggatgca gtacgtcccc gtgcaatgtt ttattcgcgc tctgtttgct
     5341 tctcgcctgc cgccaggcgt cgcttatctg tttgccgggc cgttattggt cgttgagcag
     5401 ttaaaacggc aattagcgat tattcatgaa gcgcagcgtg cccgcggcgt gccgctggat
     5461 gagggctggt atcagcgttt acgtgcgatg cccgcgttaa ttattccgct aacacacaat
     5521 gcgctaaaca atcttgctgt ccgtggtgcg gcgctggata tgcgggcatt tcggatccac
     5581 aaccgacgaa caacgttgtg ggcaccagcc gatagcaccc tacaacgcgt agcgcgttac
     5641 accatgatct tgctgatgct gacagaattc ggagcgtgga tatggttacg ttagaacagt
     5701 tccgttattg cccgacacac tcaacgcatc ctccgttctg ttatgacttt cactatgtca
     5761 aaccggggat ggtcgccatc tttggtgaca acggcagtgg caaaagtacg ctggcacagc
     5821 tgatggcggg ctggtatccg gactttctgc caggagagat aacaggcacg gggacgctcc
     5881 tcggtacgcc cattgggcat ctcccgctta atgaacaatc cgccaccatc cagctcgtcc
     5941 agcaatcgcc ctatttgcag ctctccggct gtacctttag cgtggaagaa gaagttgcct
     6001 ttgggccaga aaatctgtgt cttgctgaag cagagattat ggcgcggatt gatgccgccc
     6061 tgactctgac cgagtgccag ccgttgcgcc atcgtcaccc ggcaacactc tccggtggag
     6121 agacacagcg cgtggtcatc gcctgcgcca tcgcgatgca gccaaagcta ttaattcttg
     6181 atgaagcctt tagccgcctg acgccgcagg ccagcgaaat gctgttgcag cgcttacaac
     6241 actgggcgtt tgaacgtggt tcgctgatta ttctttttga acgtcatcgc acacctttcc
     6301 tgaactactg ccagcaagcg tggcaattgc aaaacggagc actccagcca ctatgttaac
     6361 gttaaatcag atatcttatc gctggcctgg cgcggcaacg gattgtcttt gcgacatttc
     6421 tctgcaactc aaacaaggtg aatggttggc gctgaccggc gacaatggcg cagggaaatc
     6481 gacgctgtta cgagtaatgg caggtcttct tacgcccaca gccggtacgg ttatgctgca
     6541 acaacaggcg atgaaaaacc tgaaaaaccg ccagcgcgcg gcaaagattg gcgtactgtt
     6601 tcaggaagcg gaaaaccaac tttttcacag caccgtcgct gacgagatcg catttggttt
     6661 gaaactgcaa aaatgcccgg cggatgaaat cactcaacgt acccatgctg ccctgcaatg
     6721 ttgtcagtta gcagacactg ccaacgcgca cccgttggat ctgcatagtg cgcagcgacg
     6781 gatggtcgcc gtcgcctgcc tggaagcact ctctccgcca ctattgctgc tggacgagcc
     6841 gagtcgggat tttgacgaaa attggttgag cgtgtttgaa agctggctgg agaagtgccg
     6901 ccagcgaggc acgagcgtcg tagcaatcag tcatgacgcc gcatttaccc ggcgtcattt
     6961 ctctcgtgtg gtgcgactgg aagatggtct aatcaggaac gttaatccac cagacgatat
     7021 tcacccgtag cggtcatcat taaggtcaaa tttgcaggca gactctcttc cagcacccgg
     7081 cgaacattcg gcccgtcggt acggtcataa aaagcttctg cgtctgccag cgatacccct
     7141 cccgccagct tgcgccctac cagtcgttcc cgcaacgcag tagctggcgc gttgataaag
     7201 atcgagaaat cacaaaattg cgcaagctgg caccacttct cgtcgtccag caacaaccag
     7261 tttccttcga cgatgacgag tggtgcggta acgtgcaacg catcttcaac aggatcatgc
     7321 ttttgtcgat cgtactgcgg ccacgtacaa tctccttcca cgacctggcg cagattttcc
     7381 gccagtttca caacgtcaaa tgtctctggt gcgcctttga aggggcgcaa ttgatgcgcg
     7441 tccagccagc tattgtaatg gtgaaaacca tccatcggga gcgtctgaat agcgggcagt
     7501 tccgggtctt gttgcgcgag atattcccag aaggtagtta aggtagattt acccgtcccc
     7561 ggcggagcgc aaagaaaaac caccgtacgt cgctgcgaac ttaccgcctg taaggccgcc
     7621 agcatacgca gtagcggttt atgaacattc tcaatttcat catcagagta ctgagcctgt
     7681 acctttagtc cattcaccgt cagttcgatt ttcacggcgt taattccttt aaagttgtcg
     7741 ttactgccag cgtcagcgcc gttttgacca ttccgctaaa acgcgcctct gaccagacgg
     7801 cgaaatcggc gaacttcatc gctttcaacg cgtcatagag cgtcgcattg caggttatgc
     7861 tattgacgtt gctgataaaa tcccaggtca tgtcatcggc aaatccgggc gtttcatcct
     7921 gttcgttcag gtaatgccag aactgggaaa aatgcgtgat atcggcgtac agccatttat
     7981 ccattttccc cagcgcataa atcaaacgca gtgcaacatc aatatcgtca agcgggccgc
     8041 tttgggcgag taacggcttc actgcatatt cgacgatctc ttcatcgtta tcgacaaaca
     8101 gcgacggcag aaactggcgt atcccctgga gcaaaataga atgcgcagtt ttcataaatg
     8161 aaaataaatt gtcctgtgca tccagttgct caagcacatc atcttctgtc agcgtcgcca
     8221 ttggtcttcc gatagatcac catcaatgca gggagtctat tatattacac gtttggtccg
     8281 cgccgccgat cagtaatgtc tgcgtccgcc ccccattcgc cgtctgttgg ataccgttca
     8341 ccaactcgcc gcccgtcacg cccgtagcgc taaagaggat atcgttcccc ctcaccagtt
     8401 catcgagcga gtacacacgg ttaacgtcaa cgcccattgc cttacaacgc ttgcgctcct
     8461 gctctgcaat ctgccgattt tccgtgtaat cccctttagc ctgacaaaag tcaatcagct
     8521 cggcctgcat atccccacct aatgctttaa cggcacaggc agaaatcacg ccttctggcg
     8581 caccgccgat ggtgtacatc acgtcgtaag gattatcctg ccagcaggtc agcacgctgg
     8641 cggcgacatc gccatccggc agagcgaaaa ccttcacgcc cagctgtgtt gcttcttcaa
     8701 tcgcggcact caggcgcggt ttatccagcg taaccatgcg cagcttgtcc agcggtttgc
     8761 ctaacgcccg cgccacgttg cgcaagttgt ccgccagagg caaagacaga tcaatcgcgc
     8821 ccgccgccag tcgattaaca accagctttt tcatatacat atcgggcgca tgtaacaggc
     8881 tatctcgagg ggcgaaagcc atgaccgcca gcgcattgct ctgccccatc gctaccatcc
     8941 gcgtgccttc aatcgggtca accgcgatat cgacttctgg cccatcgcct ttgcccacct
     9001 cttcgccaat ccacagcatc ggcgcatggt caatttctcc ttcgccgata accactcgtc
     9061 cacgaaaagc gacgtcgttt aatgcctggc gcatcgcggt gaccgccagg ccatcaattt
     9121 tatttttgtc gccacatccg gtttgcggcc aggcagccaa cgctgcctgt tccgtaacgc
     9181 gaaataatgg ccacgccagg gacatcattg cgcggcctcc ccaatatcaa cgccaaattg
     9241 cgccagcaga tatttctcgg cctgctcatt ccagatgccg tgcgtttcct caaccagccg
     9301 cgccagctct ttaaacagcg gatcggtttt gcctttttcg gcaaaatcgg caatggcggt
     9361 gagcggcatg gtcacgccgt tataaatgag ttttttaccg ccagggatat ccggcagatt
     9421 gagcacggta tctggcaccg catccagccc accgatatgc gtgaccataa aggacggctg
     9481 caactgcccc gtggcgctta acgcaatcgc ctctttcatg tcgtccgttg aaccgccgga
     9541 tgtaccgact acgtgcgtgc tgttgtaatg gacgttgtag aaattaaacg gcactttgaa
     9601 gtttttatcc gtcggcccgg caaagaagtt cagacagcca tcttccgcca gtaattcgtc
     9661 agccatctca acgacagcag gcaccgccgc ataaacaaaa atgtcatcga acccgacatc
     9721 tcctgtcagc gcccgcagcg tctggaccgg atcgctcatc cctttggtat tcacatacac
     9781 cagctcaatg cctttactgg ccgccagatc caccggcagc agcttctgca cctgcgccag
     9841 gcgtttgtcg tcgatatcga ccaccaccac ccgtgacggt tgtatgccac cgttaatggc
     9901 gtaatcgata gcgccaatgc ccatcggacc cgcacacgcc agcaatgcaa tattgccgcc
     9961 aggtttgacg cccatgcgat gctcataaac atattgcgtg gtgtgataat tggcatgata
    10021 agcaccaata atgcagcaca taggctctgc cagcgacgcc gcagcaaaat aagagccgtg
    10081 atacggtaat acgcagccca aattaatggc gatttcggga ataatcatat aggtggcgtt
    10141 accgccaaaa tattcgtagc tgtagcctgc tgaatatccg cttggtaacc ccatcgccgg
    10201 ttgcaataca aaacgctggc cttttttata tttgccagtg agatttttac ccacttcgac
    10261 aataaccccg gcacattcat gcccggtaat gaccggatga ttttctaaat cgtcgggtac
    10321 gcgtttatgt tcactaccga gtaacgccgc tttccaggtc gataaacaga cgctgtcaga
    10381 aattacactc actaacagtt cattatcggt aatttctggc agttcaaatt cgcgcagacg
    10441 gacatcccgc ttgccataaa tagcagcaac tttggttttc atttttacct ctgtaatcag
    10501 tgtttatgtt cggcggttaa ttgattaaat aaagtgtcga gttttggatc gccaatatag
    10561 ttattaatca atatcagcgg tttatccgtc acacgtttca cgcgcccttc cagacttgcg
    10621 tgagtgacga cgatatcggc atccgcaggc acgttttcta tcgcgtaatg ttttacttct
    10681 attgccagcc ccgctttttc caggcgttta cgaaaggtgg tcgcgcccat cgcactggag
    10741 cccatccccg catcgcaaac aaaggcgata cgcttaaccc tgcttaacga gaatgcaccc
    10801 tcttgtttca tcgccttaac cgcattggct gactgagcaa actcatcttc gctctccgtt
    10861 tccaccgttt tttccatctt cagtatcagc gaagtaatag caaaggacac cagggtacct
    10921 accgtaacac cggcaattgt cgccaggaac gagcctttcg gcgtcagtgc caggtaagca
    10981 aagatagacc ccggacttgg gccagccacc agaccaccgt ccagtaagtt aaacatccag
    11041 gtaccagaca taccgcccgc aatcatggca ataatagtca gcggcttcat cagcacatat
    11101 gggaaataca gttcgtggat cccaccgagg aagtgaataa tcatcgcccc gggcgcagaa
    11161 cgtttactca tccctttacc aaacaaggta aacgccagca gcagccccag gcccggacct
    11221 gggttagagg ccaccataaa gaagatggat ttaccgttaa ccgaagcctg ttgcattccc
    11281 agcggataat agacgccctg atcgatcgca ttattaagga aaagcacttt ctccggttca
    11341 ttgatgactg acagcagagg cagataaccc gcatgtacca gcgcctcgat acactctttg
    11401 acgaaagtat tggcaattaa caccgccggg ccgatcactt caaaacccag cagacaaagg
    11461 agcatccccg cgatacctaa tgagaagtta ttgataacca tctcaaaacc ggcaggtatg
    11521 cgtttttcca gtgccttatc gacatatttt atgaccaacc caccgagcgg ccccatgatc
    11581 attgagccaa ggaacatcgg gatctctgcg ccaacgatca caccaatagt acctattccg
    11641 cccatgaccg ccccgcgttt accgccgacc agatgaccac ctgtagaacc aatcatcacg
    11701 ggcaataaat aggtaatcat cgggccgaca attttggcga aatgttcgtt aggcagccaa
    11761 ccggtgggaa taaataacgc agtaataaaa ccccaggcaa taaaagcacc aatattgggg
    11821 atgaccattg cagtcaaaaa gcccccaaaa gcctggacct ttgcacgagc agacttgttt
    11881 tccataatat tatcctgtag aggagagagt aattatccgc ggctgataat gtccgctaat
    11941 tgtttttctg atgatgctgt taagagttgt tcgagtattt cttcttcgca cagtaattca
    12001 cttaacgcct gaatagcgcc aatgtgcgaa tcggcatcgg cagcagagag tccgatgagt
    12061 aatttaattg gttcgtcatt ccccggaaaa taaacacctt gttcaagtaa tgtcaaagac
    12121 atcccggttt taagcgcccc acattccggt cgcgcatgag gcattgccac gcctggtgcg
    12181 agaatataat aagggccatt gttaatggtg gaatctttaa ttgcctgaat gtaattctcg
    12241 ctgatatagt ttttatccag caatgatacc atcgagaaat cgatagcttc ctgccaatct
    12301 tttgctgaat gtataactga gattgatgat tctggaaaat aatcactaag ccgcataaat
    12361 tatccttatt ttatttttag ggtaccgtcc ggcggtaaaa ataaaatact cgtgcggttt
    12421 aatgaaagc
//
LOCUS       DAZKIV010000141        12031 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZKIV010000141 DAZKIV010000000
VERSION     DAZKIV010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.141"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            220..990
                     /gene="yjjP"
                     /locus_tag="ACHOT6_003778"
     CDS             220..990
                     /gene="yjjP"
                     /locus_tag="ACHOT6_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFP9948930.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            981..1454
                     /gene="yjjB"
                     /locus_tag="ACHOT6_003779"
     CDS             981..1454
                     /gene="yjjB"
                     /locus_tag="ACHOT6_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFP9948931.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSESLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1562..2101
                     /gene="dnaT"
                     /locus_tag="ACHOT6_003780"
     CDS             1562..2101
                     /gene="dnaT"
                     /locus_tag="ACHOT6_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFP9948932.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNTPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2104..2841
                     /gene="dnaC"
                     /locus_tag="ACHOT6_003781"
     CDS             2104..2841
                     /gene="dnaC"
                     /locus_tag="ACHOT6_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFP9948933.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2890..3384
                     /gene="yjjA"
                     /locus_tag="ACHOT6_003782"
     CDS             2890..3384
                     /gene="yjjA"
                     /locus_tag="ACHOT6_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFP9948934.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3638..5929
                     /gene="opgB"
                     /locus_tag="ACHOT6_003783"
     CDS             3638..5929
                     /gene="opgB"
                     /locus_tag="ACHOT6_003783"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFP9948935.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6068..7090)
                     /gene="lgoD"
                     /locus_tag="ACHOT6_003784"
     CDS             complement(6068..7090)
                     /gene="lgoD"
                     /locus_tag="ACHOT6_003784"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFP9948936.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPVLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7229..8143
                     /gene="lgoR"
                     /locus_tag="ACHOT6_003785"
     CDS             7229..8143
                     /gene="lgoR"
                     /locus_tag="ACHOT6_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFP9948937.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8358..9719
                     /gene="lgoT"
                     /locus_tag="ACHOT6_003786"
     CDS             8358..9719
                     /gene="lgoT"
                     /locus_tag="ACHOT6_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9948938.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9768..11432)
                     /gene="tsr"
                     /locus_tag="ACHOT6_003787"
     CDS             complement(9768..11432)
                     /gene="tsr"
                     /locus_tag="ACHOT6_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFP9948939.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11551..11997)
                     /gene="hpaR"
                     /locus_tag="ACHOT6_003788"
     CDS             complement(11551..11997)
                     /gene="hpaR"
                     /locus_tag="ACHOT6_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFP9948940.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2924 a         3173 c         2929 g         3005 t
ORIGIN      
        1 taaaggccaa acaataaatt cattatgatg ataatattca tgtgatagtt tgtttgcggc
       61 gagagataat tcgcttttta tcaccgagct tctcccgata accaggatta atcagcgcaa
      121 ttttgatctt aacctaccgc aaaacaaccg tgcttcatga tacaaatgga ttctcattat
      181 ctgaacaata cgcagcacgt ctatgacaaa gggcgagtta tgcaaactga gcaacagcga
      241 gccgtaacgc ggctttgtat ccagtgtgga ttatttcttt tgcaacatgg tgcggaaagc
      301 gcgttggttg atgagctttc ctcacgactg ggtcgggcgc tgggaatgga cagcgttgaa
      361 agttctatct cttcgaacgc catagtgctg acaaccatta aagatgggca atgcctgaca
      421 tcgacacgta aaaatcacga tcgcggcatt aatatgcatg tggtgactga agtccagcac
      481 attgtgattc tggcggagca ccatctgctg gattacaaag gcgtagagaa acgatttagc
      541 caaattcagc cattacgtta cccaagatgg ctggtagcct taatggttgg cctttcttgt
      601 gcctgtttct gtaaactcaa taaaggtggc tgggatggtg ccgtcatcac cttctttgcc
      661 agtacggccg cgatgtatat ccgccagctg ctggcacaac gtcatcttca tccacaaatc
      721 aacttttgcc ttaccgcttt cgccgccacc accatttccg gattgctttt gcaactcccc
      781 actttcagca atacccccac cattgcgatg gccgccagcg ttctgctgct ggtgccggga
      841 tttccgttga taaatgccgt cgccgatatg tttaaaggcc acatcaatac cggactggca
      901 cgctgggcga tcgccagtct gctgacactg gctacctgcg tcggcgtggt gatggcactg
      961 acgatttggg ggctacgcgg atgggtgtga tcgagtttct gtttgcattg gcgcaggata
     1021 tgatcctcgc cgccattcca gcggtcggct ttgcgatggt gttcaacgtt cccgttcggg
     1081 cattacgctg gtgtgcgctg cttggcgcga ttggtcatgg ttcacgaatg atcttaatga
     1141 ccagcgggct gaatattgag tggtcaacct ttatggcttc tatgctggtc ggcaccattg
     1201 gtattcaatg gtcgcgctgg tatctggcac atccgaaagt gtttaccgtg gcggctgtta
     1261 tccctatgtt ccctggcata tcggcttata ccgcaatgat ttcggccgta aaaatcagcc
     1321 agttaggtta cagcgagtca ttgatgatta ccctgttaac caattttctt acagcttcat
     1381 cgattgttgg tgcgttatcc atcggtcttt ccatccctgg attatggttg taccgcaagc
     1441 gccctcgcgt ataaaattgc ctcgcttaaa gcacgcggat gagagacagc cacctctcct
     1501 ccgtgtgtta ctataaaagt tatctccctt ctcgttcatc gttccatatt tgagaaacag
     1561 tatgtcttcc agagttttga ccccggacgt cgttggtatt gacgccctgg tacacgatca
     1621 ccaaactgtt ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta acaatacccc
     1681 ggcgttttat gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg aagaaaagct
     1741 ggcgcgtccg ggaagcgatg tcgctctgga cgatcaactc tatcaggaac cgcaagccgc
     1801 tcccgttgct gtacccatgg ggaaattcgc catgtatccg gactggcaac ccgatgccga
     1861 ttttatccgc ctggcggcgc tatggggtgt ggcgctaaga gagccggtga ctaccgaaga
     1921 actggcctca ttcattgcct actggcaggc ggaaggtaaa gtctttcacc atgtgcagtg
     1981 gcaacaaaaa ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg gactgccgaa
     2041 acgagatgtg aatacggtca gtgaacctga cagccaaatt ccaccaggat tcagagggta
     2101 acgatgaaaa acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc tgcccatatc
     2161 aaacctgcat tcaaaacggg tgaagaacta ctggcctggc agaaagaaca aggagcgatc
     2221 cgctccgccg ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac ctttaaccgc
     2281 tccggtattc gtccactgca tcagaactgc tcctttgaga actatcgcgt tgagtgtgaa
     2341 gggcagatga atgcgttaag caaagcgcgc cagtatgtcg aagagtttga cggcaacatc
     2401 gccagcttca tcttttctgg taagccagga accggcaaaa accatctggc ggcggcaatc
     2461 tgcaacgagc tgctgctacg cggtaaatcc gtattgatca tcaccgtggc cgatattatg
     2521 tcggcgatga aagatacctt caggaatagc ggtaccagcg aagaacaact gcttaacgat
     2581 ctgagcaacg ttgatctgct ggtgatcgat gagatcggcg tgcagaccga atcgaaatac
     2641 gaaaaagtga tcatcaacca gatcgtcgat cgccgctctt cttccaaacg cccaacaggt
     2701 atgctgacca acagtaatat ggaagagatg accaagttgc tgggcgaacg cgtgatggac
     2761 cgtatgcgcc tgggtaacag tttgtgggtg atcttcaact gggatagcta ccgtagccgt
     2821 gtaacaggta aagagtatta atccccgttt ctggcggtat actgcgacac aattccacct
     2881 tgagtaatga tgatgaaaac tgttaaacat cttctgtgct gtgccattgc cgccagcgca
     2941 ttaatttcca ccggggtgca tgctgcgtcc tggaaagatg cgctctccag cgccgccagc
     3001 gaacttggca accaaaacag tacgacacag gaaggcggtt ggtcgctcgc gtcattaact
     3061 aacttgctta gcagcggaaa ccaggcctta agcgcagata acatgaacaa tgccgcaggc
     3121 attctgcaat actgcgcgaa gcaaaagctg gcttcggtaa ccgatgccga aaacatcaag
     3181 aaccagatgc tggaaaagct gggcctgaac agtgaagagc aaaaagaaga caccaactat
     3241 ctggacggca ttcagggttt gctgaaaacc aaagatggtc agcaactcaa tctggataac
     3301 atcggaacga ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct ggtgttaaag
     3361 caggggctga acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt tacccggctg
     3421 acgcggctct ctcctgccct tttccctgct cctggacggt tttccccctg ttacacggga
     3481 agcgaccgcg atcaaaaaag tctgtttata aactaattcc taacaacatc gcattttttt
     3541 gacactagaa ttgcagcaat atggcgacgc cattacattg tgcttctcaa aaagcttaaa
     3601 cgttggctaa acggcccggt ttagtgagga tcgcccgttg tcagaactac tctctttcgc
     3661 cctttttctc gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca cctggtggtt
     3721 tgcagccaca ttaacggtgc tggggctatt tgtcgtttta aatatcaccc tgtttgccag
     3781 cgactatttt actggcgatg gtattaacga cgcggttctc tataccttaa ccaacagcct
     3841 gaccggtgct ggcgtcagca aatacatcct gccgggtatc ggcattgtgc tggggctgac
     3901 agcggtgttc ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc cgcaccattt
     3961 tggttacagc ctgctggcgc tcttactggc gctgggttcg gtggacgcca gcccggcatt
     4021 tcgtcagata acggaactgg tgaaatccca gtcacgcgat ggcgacccgg actttgcggc
     4081 ttattataaa gagccgtcga aaactatccc tgacccgaaa ctcaacctgg tttatatcta
     4141 cggcgaaagt ctcgagcgga cctattttga taacgaagct ttcccggatc tcacgcctga
     4201 actgggcgcg ttgaaaaatg aaggcctgga tttcagccac acgcagcagc tgccaggaac
     4261 ggattacacg attgcgggca tggtggcttc tcagtgcggc ataccgctgt ttgccccctt
     4321 tgaaggcaac gcctccgcct ctgtctccag cttcttcccg cagaacatct gtctgggcga
     4381 tatcctgaaa aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc tgcgttttgc
     4441 cggtaaagat gtgttcctga agtcgcatgg cttcgaccac ttatacggct cagaagagct
     4501 gaaaagcgtg gtggccgacc cgcactatcg caacgactgg ggattctacg acgataccgt
     4561 tctcgatgaa gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc gattctcact
     4621 gtttaccctg acagtcgata cccatcaccc ggatggtttt atctcgcgta cctgtaaccg
     4681 caaaaaatat gattttgacg gtaagccgaa ccagtcattc agcgcggtaa gttgcagcca
     4741 ggagaatatc gcggcgttta tcaacaaaat caaagcgtca ccgtggttta aagataccgt
     4801 catcgtcgtc tcttctgacc atttagcgat gaacaacacg gcgtggaaat accttaataa
     4861 gcaggatcgc aataacctgt tttttgtcat tcgtggcgac aagccgcagc aagagacgct
     4921 ggcagtgaag cgtaacacga tggataacgg cgcgacggtg ctggacattc tcggtggcga
     4981 taactatctc ggacttggtc gtagcagttt atccgggcag tcgatgtcgg aaatcttcct
     5041 caatatcaaa gagaaaacat tggcgtggaa gccggatatc atccgcctgt ggaaattccc
     5101 taaagagatg aaagagttca ctatcgacca gcagaaaaac atgattgcct tctcgggtag
     5161 ccatttccgt ttgccgctgc tgttgcgggt ttcagacaag cgcgtagaac cgctgccgga
     5221 aagtgaatac tcagcaccgc tgcgtttcca gctggccgat ttcgctccac gcgacaattt
     5281 cgtctgggtt gaccgttgct acaagatggc acaactttgg gctccggaac tggcactctc
     5341 caccgactgg tgtgtctcgc aggggcagct tggcggtcag caaattgtcc agcatgttga
     5401 taaaacaatg tggaagggca aaacggcatt taaagatacg gtcatcgaca tggcgcgtta
     5461 caaaagcaat gtcgatacgc tgaaaattgt tgataacgat attcgctaca aagccgacag
     5521 tttcatcttc aacgtcgccg gtgcgccaga agaggtgaaa cagtttagcg ggatttcccg
     5581 tccggagtcg tggggccgct ggtccaacgc tcagctgggc gatgaagtaa aaatcgagta
     5641 caagcatccg ctgccgaaga aatttgacct ggtgattacc gccaaagcat acggcaataa
     5701 cgccagccgt cctattccgg tacgcgtagg caatgaagaa caaactcttg tgctgggcaa
     5761 tgaagtaacc accaccacac tgcatttcga taacccaacc gatgccgaca cactggtaat
     5821 tgtgccgccg gaacctgtct caaccaacga agggaatatc ctcggacact cgccgcgtaa
     5881 gctcgggatc ggcatggtgg aaattaaagt tgtggaacgt gaagggtaat tcctgactga
     5941 tgtgtaggtc gcataaggtg cgacctacac accttctccg gcctacaaaa catcattgag
     6001 aaacagaaaa aaacagcgcc caacacttcc ccggacgctg catggcaact ttaataatct
     6061 cgccttatca gaaagtaatt acacccttaa tcaactcacg attgttaatc acatcgcgct
     6121 cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt taacatcatg tcggcagtga
     6181 ttttcccttc tgccatcagt cgaccgactt tagcgaaatc ttccggcgtg gcgttgcggc
     6241 tgcccatcat cgtcgtttct tttttatgga attccggatc ggaaaactgc aactcacctt
     6301 taaacaggcc gacaaatacc accgtgccgc cgtgacgaat cagattcacg gtgttattca
     6361 tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc cagcgaacca ccaaactgcg
     6421 cccgtagctg cgcgtcaaaa tcttcggctg acgggtccag tacaggtaat tccagacgcg
     6481 ttgccacatg ttcacggcgc gctggactgg tatccgccac caccacctgt gcgccatcgg
     6541 ctttagcgat tgccgccgcg cccagaccga ttggccccgc cccgaccacc agcacctgct
     6601 cgccgggagc aatggctgcg cgacgcaccg catgagcgct aatagcgaaa ggttcaatca
     6661 atgctgccgc ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc ggcacgctca
     6721 aatactcact aaaaccgcca tcctgatgca cgccaatgac tgaaattttt tcacagcaat
     6781 tggtacgccc gcttttacac gccgggcatt gctgacaggc aacataaggg atcacagcaa
     6841 cttgctgacc atttttaaga tcagcaatat ttttacccag cccaacaatc tccccacata
     6901 tttcatggcc taaaacacgt ggataactaa aaaatggttg atttccaccc caggcatgaa
     6961 tatcggtacc gcaaatcccg acagacttaa tttttattaa tgcttcattg tcacccggaa
     7021 taggtatctc gcgttgtttc cagactaatt ctttcggctg ctggcaaatt aaaacattca
     7081 tcgtagacat gattcggctc tccatttttt gttgccttag atatcaatga aaatgaaaaa
     7141 tggcagcgga acggaaaatc tgttttgtga aaacacacgc ataaaaatgt tttaaatcgg
     7201 gttttaatgg actcacaagg aggaaaatat gagtcgttca caaaatttac gccacaatgt
     7261 gattaaccag gtcattgatg atatggcgcg gggtcatatc ccttccccac ttccatcgca
     7321 aagtgcgctg gcggaaatgt acaacatcag ccgcaccaca gtgcgtcata ttctcagcca
     7381 tttacgcgaa tgcggcgtct tgacgcaggt tggaaacgac tatgttattg cgcgtaaacc
     7441 tgaccacgat gacggtttcg cctgtactac cgcttcaatg agtgaacaaa acaaagtgtt
     7501 tgagcaggct tttttcacga tgataaacca gcgccagtta cgcccagggg aaactttttc
     7561 tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtg gttgtaagag aatatctttt
     7621 aaaattcggc cgttataatc ttattcaaag cgaaaagcgc ggccagtgga gcatgaagca
     7681 attcgatcaa tcctacgccg agcagctgtt tgagctacgc gaaatgctcg aaacacattc
     7741 acttcagcac tttctcaacc tgcctgatca tgatccccgc tggctgcagg cgaaaaccat
     7801 gctcgaacgt catcggctac tgcgtgacaa catcggcaat agctttcgca tgttctcgca
     7861 acttgacaga gattttcact ccttactgct ttcagctgcc gacaatatct tttttgatca
     7921 atcgcttgag atcatctcag tcatctttca ttttcactat caatgggacg aaagcgatct
     7981 taaacagcgc aatatcattg ccgtcgatga gcatatgacc attctcagcg ctctgatttg
     8041 ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat ttgaattcag ctaaacaatc
     8101 aatgattcga tccattaatg agaacacgcg ttatgctcat taagtacgga ttaaaaacag
     8161 caaactatta gcgggtgcac ggttttatca aaagataaat accgggaaat tcccgcttac
     8221 ctatgctcac aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag tcctaaaact
     8281 ttaacctgcc gttaatgtca accttgcgta atgcacaatt acgtaaacaa ttaaaattat
     8341 aaatacagga gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt tcttcattta
     8401 ctccatcttc gtcggcagat attcccgtgc caccagatgg attagttcaa cgcagtaccc
     8461 gaattaaacg cattcaaacc accgccatgt tgttattatt ttttgcggcg gtaatcaatt
     8521 atctcgaccg cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa ttgggattaa
     8581 gtgccaccga aatcggcgct ttgctctccg tgttttcact cgcttacggg attgcgcaac
     8641 ttccttgcgg cccactattg gatcgtaaag gcccacgcct gatgctggga ctggggatgt
     8701 tcttctggtc actgttccag gcaatgtctg gcatggtgca caactttacg cagttcgtgt
     8761 tggtgcgtat cggtatgggg attggtgaag cgccgatgaa cccatgcggt gtaaaagtca
     8821 ttaacgactg gttcaacatc aaagagcgcg gacgcccgat gggcttcttc aacgcagctt
     8881 ctaccattgg cgttgccgta agcccaccga ttctggcggc gatgatgctg gtgatgggct
     8941 ggcgcgggat gtttattacc attggtgtac tggggatttt tctcgccatc ggctggtata
     9001 tgctctatcg caaccgcgag cacgtagaac tgactgccgt tgaacaagct tatctcaatg
     9061 caggtagcgt caatgcccgc cgagatccgc tcagttttgc cgaatggcgc agcctgttcc
     9121 gtaaccgtac aatgtgggga atgatgctcg gattcagtgg catcaactac actgcgtggc
     9181 tgtatctggc ctggcttcct ggttacctgc aaacagccta taacctggat ttaaaaagca
     9241 cagggttgat ggcggctatc cctttcctgt ttggggctgc cgggatgctg gtcaacggtt
     9301 acgttactga ctggctggtc aaagggggaa tggctccgat taaaagccgt aagatctgca
     9361 ttattgccgg gatgttctgt tctgccgcct ttacgctggt cgtaccgcaa gcgacaacat
     9421 ccatgacagc ggttctgctg attggcatgg cactgttctg tattcacttt gccggaacat
     9481 cctgctgggg cttgatccac gtcgcagttg cttctcgcat gactgcgtcg gtgggcagta
     9541 tccagaactt tgccagcttc atctgcgcct cttttgcgcc gatcattact ggttttattg
     9601 ttgataccac ccactcattc cgtctggcac taatcatctg cggttgcgtc accgcagcgg
     9661 gggcactggc gtacatcttc ctggttcgtc agccgatcaa cgacccacgt aaagattaat
     9721 ctccctatgc ccgataacat tttgcttatc gggcattttt atggcgatta aaacgtttcc
     9781 cagttctccc cgctatctgc cactgccatt ttacgcggcg tagctggcgt cacggttttt
     9841 accacagccg atgtttcacg ctgctgttgc tgctgttgct gaatccggaa cactgccaca
     9901 gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg cggcggcggc agattcttcc
     9961 accagcgcgg cgttctgttg agttacccgg tccatctcag caaccgctaa gccaacctga
    10021 tcgataccac ggctctgctc atcagaagca gaagctattt cgcccataat gtccgtcacg
    10081 cgggtcacag cactgacaat ctccgccatt gtttccccgg cgctttcgac cagcgtagag
    10141 ccaacatcca ctttccccac cgagtcttca atcaggcttt taatttcacg agccgcctgg
    10201 gcgctacgct gggccagatt acgcacttct cccgcaacca ccgcaaaacc gcgcccttgc
    10261 tcacccgcac gcgcagcctc aaccgccgcg ttcaaagcca gaatattggt ctggaaggca
    10321 atgccgtcaa ttacgctgat aatatcggcg attttctgcg aactggtgga gatatcgcgc
    10381 atagtctgca ccacgttatc caccactttg ccgccgcgtt gcgccgtttc agaagcactt
    10441 aacgccagat ggctggcctg gcgcgcattt tcggcgttct gcttcaccgt tgcggtcagt
    10501 tgctccatgc tggctgccgt ctcttccagc gaagcggcct gttgctcggt gcgcgaagag
    10561 agatcgttat tgccagtggc gatttcgctg gcaccgctat agatggcatt ggccccgttg
    10621 cgcacatcac cgacggtacg catcagctct ccctgcatat ggcgcaaact ctctgccagt
    10681 tgccccatct cattagagcc atccacctca atcggtttca ccagatcgcc gcctgcaata
    10741 tgacgaatgc tgtcaatcag gcgattcatt ggcgctacca gcgaggcttt aataccgaac
    10801 cagacggcga agatgaccgc cagtacgacg atcatcacgc ccaccagaat ccacatagcc
    10861 tggctgtagg aggcattgtt atcgctgacg gcgatatcat agagccgatc gttttgctcc
    10921 atgtaagcca catactgctt ctcgaaaccg tcctgatatc cctgggtcgg ctgatcaaag
    10981 aactcgttga ttttgcctgc acctaatagt tggatcagct ccgccagcgc attgtgataa
    11041 atatcgtaat tacgtttgat ctctgccgct gcggcggtgc tctgacgcgg gtcacgcggc
    11101 aacgcttcgt aatccgccca gtttttttcc gcctgtttca gcgaaatact ggcactctgc
    11161 atcagctcag caacggttga accgctacca atattattct gatccatcat gtagcggata
    11221 cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga cccagctgcc attcagcgtg
    11281 gattgctgct ggcgaatggt ttgtaaaaca gtgaaatttt ctttgtcatt ctttaaggca
    11341 ttaaagaaca gaccgcctga tgtcagttgt aaaaggccaa aaacagccaa aaccagcagt
    11401 aagctggtca cgatcttgat acgttttaac atggtttctc tttcctgtgg accgatgcaa
    11461 gattttcggc ctggaaagca aaaactttat gaaaaggtgt aacccgccat ccatggaaaa
    11521 accaggcgct ggttatcaga tactaaaaag ttattcatta tcgcccggga tgtcttcttg
    11581 tcgggaattg cccagagcaa taaattcctc taacagatgg gttaactgtt gcattttctc
    11641 ggcagtaaac tgagcttcaa tctgccgata ggcttcttca atctgtgtct gagcgcggtt
    11701 atacagcgcc tgcccctctt tggttagcga gatatagagc ttccgctgat cattgatcgg
    11761 ctttaatcgc aacactaaac cgtcgcgttc catccgcgta aggatcccgg tcaggcttgg
    11821 gcgcaaaatg caggcgcgat acgccagatc gtgaaaatcc attgatgggc tttccgccag
    11881 aatacgcacg atgcgccact gttgctcagt caaattatgc cgtttaacga ttgggcgaaa
    11941 ataactcatc gccgcttctc gcgcctggag caacgcaatg gttagagagt cgtgcattat
    12001 ctttcccctg aaaaattcaa aatcattaat a
//
LOCUS       DAZKIV010000142        11791 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZKIV010000142 DAZKIV010000000
VERSION     DAZKIV010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11791
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.142"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..331)
                     /locus_tag="ACHOT6_003789"
     CDS             complement(50..331)
                     /locus_tag="ACHOT6_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFP9948941.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(594..1703)
                     /gene="apbC"
                     /locus_tag="ACHOT6_003790"
     CDS             complement(594..1703)
                     /gene="apbC"
                     /locus_tag="ACHOT6_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFP9948942.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1835..3868
                     /gene="metG"
                     /locus_tag="ACHOT6_003791"
     CDS             1835..3868
                     /gene="metG"
                     /locus_tag="ACHOT6_003791"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFP9948943.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4009..7803
                     /locus_tag="ACHOT6_003792"
     CDS             4009..7803
                     /locus_tag="ACHOT6_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFP9948944.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIEWDYANNVIIILPSGIAPSYLEQYSRFELR
                     LRKHLSLTEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLIIRVAGHTKRCHDRMTKAIAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVRGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWIKGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7813..11445
                     /gene="yehI"
                     /locus_tag="ACHOT6_003793"
     CDS             7813..11445
                     /gene="yehI"
                     /locus_tag="ACHOT6_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFP9948945.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRKWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSVPARTMAALACIAQ
                     NDNQQLLDEIVQQEGLEYATEVVIARQFIARCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIAALPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVATDPKAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQAQSVVKRYLSASPKPVIDSTDNSGLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQVYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPEAQLAEQALRSGHIEALINIWEND
                     NYSRYRPEKSVWNLYLLAQLPREMALTFWLRINEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFARDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFNQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHSLFVNHPFTRLVTQRLIWG
                     VYPANEPRCLLKAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTVEMRSEFAQLF
                     ADYEIMPPFRQLSRRTVLLTPDESTSNSLTRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYRDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11507..>11791
                     /gene="yehK"
                     /locus_tag="ACHOT6_003794"
     CDS             11507..>11791
                     /gene="yehK"
                     /locus_tag="ACHOT6_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFP9948946.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEINKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRE"
BASE COUNT         3078 a         3004 c         2918 g         2791 t
ORIGIN      
        1 atacaaatga tttcggcttt ctaaatgcaa ttaaaatcag gttgccaaat taatcgtagc
       61 gatttaacac gattcgacgt ttgctatcgc tacctgcaac aaccacttct gtcgttacac
      121 ctttgacggg cgaagaaaac agatgacggg tgtcaacatt atacgttaac tgaccacaat
      181 gctgttgagg ttgaccatta accatagcca tactgcatgt tgggggactg atcctgccat
      241 taatcgctaa tgtggcagtc tctgaagaga atgcgggaga agccaatcca taggcaagaa
      301 aaataattcc tgacagccaa tacttattca ttgaacgtta tccctgtagt aaaggttatg
      361 cccggataga atgagtgcat aacaaactat agctgtacat ccactacaca gccacgaagg
      421 atgataatga agcattgcct gtatgatcaa tcgactttgt agagtttcgg acgaaggtcc
      481 gcagaatatt cgcagtatta aataagtgtt cattaatgcg gcaaaaacta atacaccgca
      541 tcaatgtaac atctctgcgg tgtatgaaga ataaccagaa tggttaatag gcgttagacc
      601 gcgcggaagg aaatctcgcc tggaatgact tcaccctgcc agtagagctg agctgcaacg
      661 cggtcagcca gctggcggta gatagcggta aattcgctct ccggacggct gataaccgtt
      721 ggcgtgcctt tatcgagatc ctcacgtaag gagatgtgta acggcatctg ccccagcagc
      781 tgtgtgtggt atttttcagc cagtttctca gcaccaccgg taccgaaaat cggttcatgg
      841 tgaccacagt tactgcaaat atgcacactc atgttctcga caataccaag caccggaacc
      901 tcgactttct cgaacatcac aatgcctttt ttcgcatcga tcaacgcgat gtcctgcggg
      961 gtggtgacca cgactgcacc agttactgga atgttttgcg ccagcgtcaa ttgaatgtca
     1021 ccggtgcctg gcggcatatc aagcaccaga tagtcgagat ccggccacaa ggtttcctgc
     1081 aacatctgca ttaacgcctt gctcgccatc ggtccacgcc acaccatcgc attgtcgtca
     1141 gtgaccagat aaccaattga attggttgcc aggccatgag acataatcgg tgccatgtgg
     1201 gtaccgtccg gcgacgttgg acgctgattt tctgcgccca gcatggtggg aattgatggg
     1261 ccgtagatat cggcgtccag aatgccgact ttcgcccctt ccgccgccag tgccagcgcc
     1321 aggtttaccg ccgtggagga tttacccacg ccgcctttac cggaactgac cgcaatgatg
     1381 ttcttcacac cattaatacc cggctgattc ttcacgcgtt ttaaggtggc gatattgtgc
     1441 gacagtttcc agtcgatagc tttcgccccg gtgatacgca gcagttcggc gctacattgc
     1501 tcttttaact cttcaaacgc gctatgccag acgaatggca taaccagttc cacatgcaac
     1561 gtgtcgtcca tccaggcgac atggtgcaac gctttcagcg tggtgaggtt atgcttcagg
     1621 gttgggtgct gaaaattggc cagtgtcccg gcgaccattg cgcgcaaggc ttctggtgat
     1681 ttggcctggg attgttcgtt catcccgact ccttttttgt atagataaac catcagctga
     1741 tagtttacct gaagaatata gagaagtact tacttaacat tttcccattt ggtactatct
     1801 aacccctttt cactattaag aagtaatgcc tactatgact caagtcgcga agaaaattct
     1861 ggtgacgtgc gcactgccgt acgctaacgg ctcaatccac ctcggccata tgctggagca
     1921 catccaggct gatgtctggg tccgttacca gcgaatgcgc ggccacgagg tcaacttcat
     1981 ctgcgccgac gatgcccacg gtacaccgat catgctgaaa gctcagcagc tcggtatcac
     2041 cccggagcag atgattggcg aaatgagtca ggagcatcag actgatttcg caggctttaa
     2101 catcagctat gacaactatc actcgacgca cagcgaagag aaccgccagt tgtcagaact
     2161 tatctactct cgcctgaaag aaaacggttt tattaaaaac cgcaccatct ctcagctgta
     2221 cgatccggaa aaaggcatgt tcctgccgga ccgttttgtg aaaggcacct gcccgaaatg
     2281 taaatccccg gatcaatacg gcgataactg cgaagtctgc ggcgcgacct acagcccgac
     2341 tgaattgatc gagccgaaat cggtggtttc tggcgctacg ccggtaatgc gtgattctga
     2401 acacttcttc tttgatctgc cctctttcag cgaaatgttg caggcatgga cccgcagcgg
     2461 tgcgttgcag gagcaggtgg caaataaaat gcaggagtgg tttgaatccg gcctgcaaca
     2521 gtgggatatc tcccgcgacg cgccatactt tggttttgaa attccgaacg cgccgggcaa
     2581 atatttctac gtctggctgg acgcaccgat tggctacatg ggttctttca agaatctgtg
     2641 cgacaagcgc ggcgacagcg taagcttcga tgaatactgg aagaaagact ccaccgccga
     2701 gctgtaccac ttcatcggta aagatattgt ttacttccac agcctgttct ggcctgccat
     2761 gctggaaggc agcaacttcc gcaagccgac caacctgttt gttcatggct atgtgacggt
     2821 gaacggcgca aagatgtcca agtctcgcgg cacctttatt aaagccagca cctggctgaa
     2881 tcattttgac gctgacagcc tgcgttacta ctacactgcg aaactctctt cgcgcattga
     2941 tgatatcgat ctcaacctgg aggatttcgt tcagcgtgtg aatgccgata tcgtgaacaa
     3001 agtggttaac ctggcctctc gtaatgcggg ctttatcaac aagcgttttg acggcgtgct
     3061 ggcaagcgaa ctggctgacc cgcagttgta caaaaccttc actgatgccg ctgaagtgat
     3121 tggtgaagcg tgggaaagcc gcgaatttgg taaagcagtg cgcgaaatca tggcgctggc
     3181 tgatctggct aaccgctatg tcgacgagca ggctccgtgg gtggtggcaa aacaggaagg
     3241 ccgcgatgcc gacctgcagg caatttgctc aatgggcata aacctgttcc gcgtgctgat
     3301 gacttacctg aagccggtac tgccgaaact gaccgagcgc gcagaagcat tcctcaatac
     3361 agaactgacc tgggatggta tccagcaacc gctgctgggc cacaaagtga atccgttcaa
     3421 agcgctgtat aaccgcatcg atatgaagca ggttgaagca ctggtggaag cgtctaaaga
     3481 agaagtgaaa gcagctgccg cgccagttac tggcccactg gcagatgatc cgattcagga
     3541 aaccatcacc tttgacgact tcgcaaaagt tgacctgcgc gtggcgctga tcgaaaacgc
     3601 agagtttgtt gaaggttctg acaaactgct gcgcctgaca ctggatctcg gcggtgaaaa
     3661 acgcaatgtc ttctccggta ttcgttctgc ctacccggat ccgcaggcac tgatcgggcg
     3721 tcacaccatt atggtggcca acctggcacc gcgtaaaatg cgctttggta tctccgaagg
     3781 catggtaatg gctgctggtc ctggcgggaa agatatcttc ctgttaagcc cggatgctgg
     3841 tgctaaacca ggccatcagg tgaaataatc ccccttcaag gcgctgcatc gacagcgcct
     3901 tttctttata aattcctaaa gttgttttct tgcgattttg tctctctcta acccgcataa
     3961 atactggtag catctgcatt caactggata aaattacagg gatgcagaat gagacacttt
     4021 atctatcagg acgaaaaatc acataaattc tgggcagttg agcaacaggg aaacgagttg
     4081 catatcagtt ggggaaaagt tggcaccaaa gggcaaagcc agataaaaag tttttcagat
     4141 gctgcggcag cggcaaaagc ggagcttaag ctgattgcgg agaaggtgaa gaaggggtat
     4201 gtggagcaag cgaaggataa ttctttgcaa ccttcccaaa cggtaacggg ctctctcaag
     4261 gtagcggact tatccaccat tattcaggaa caaccctctt tcgtagcaga aacccgtgcg
     4321 cctgacaaaa atacagatgc tgttttaccg tggctggcga aagatattgc tgtcgttttt
     4381 ccgcccgaag ttgtacacac cacgttaagt catcgccgct ttcccggagt tcctgttcag
     4441 caagcagaca aattgaccca attacgtcgc ttagcctgta gtgtgtcgca acgggataat
     4501 aaaacagcca catttgactt cagcgcctgt tctttagaat ggcaaaacac cgtcgcccag
     4561 gcgatcagtc agatcgacgg cctgaaaaca acacagttac catcaccagt aatggctgta
     4621 ctcacggcac ttgaaatgaa atgcacaaga tataaagtgc gtgaggatgt tatggatcag
     4681 atcgtccagg aaggcggtct ggaatatgct actgatgtaa taatacacct tcaacagatt
     4741 gatattgaat gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct
     4801 agctacttgg agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactgacg
     4861 gaagagtctc tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca
     4921 gaatggcggc aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa
     4981 attgcccagc gtctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg
     5041 gcgactgatg agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt
     5101 gatgattact attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca
     5161 gccctgcccc gatttgctcc ctatgccgca agtgactact gtgccgatgt gttgcgtcat
     5221 atcaatcatc cgttcgcatt aacactgatt atacgtgtag ccgggcatac taaacgctgt
     5281 cacgatcgga tgacgaaagc cattgctgcg ttcccacatg cagcaatggc ggcactgacg
     5341 gaacttcttg ggcaaaaaga agagaacagt tggcgcatta tgctaatgac aatgcttatc
     5401 tcacaaccag cactggcaga acaggtcatt ccctggctct cgacacccgc agttgccgta
     5461 ctgaaatcat gccagcaaca actgacacag ccctcaaacc atgccagcgc cgatctactg
     5521 ccagccgtag tagtctcccc tccctggctt tcgaaaaaga aaaaatcgcc gattccggtg
     5581 ctggatttag cgccattagg cattgagcca atctgttatc tgacagaaga aatcagtaat
     5641 caacttttgg cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt
     5701 actaccaacc tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa
     5761 gctcccgaag cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa
     5821 tttaaggtgt ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg
     5881 ccgctggaga aagcagtaaa agcatggaat gcccttaccc tatctccaca taccgatacc
     5941 gaatacgcca tgttacattt tggactcaaa gggttacctg ggttagtaaa ctcacttgca
     6001 cgctatccac aagaagcctt gcccatcacg aattacttcg cagcgagtga gctggctcct
     6061 gccgtcgccc gtgccttcaa caaactgaaa acgctacgcg aaaacgcccg tagctggctg
     6121 ttgaaatacc cggaacatgc ccttaccggc ctgctgcctg cggcgctcgg caaagccggt
     6181 gaagcacagg ataacgcccg cgctgccttg cgtatgctta ccgaaaacgg tcatcagcca
     6241 ttactgcaag aaatcgcccg acgttataac cagccggaag taaccgatgc ggtgaacgct
     6301 ctgcttgcgc tcgatccctt agataatcac ccgacaaaaa tccccactct tccggccttt
     6361 tatcagccat cgctctggac gcgcccggta ttaaaagcaa atgcccaatc actgccagat
     6421 agcgccctcc tccacctcgg tgaaatgctc cgcttccctc aggaagaggc tctgtatccg
     6481 ggattattgc aggtgaaaga cgtctgttcc gccgactcac tggcgggatt tgcctgggat
     6541 ctgtttaccg cctggcagac cgctggcgcg ccgtcgaaag agagttgggc gttcactgcg
     6601 ttaggcgttc gcggtaacga tgacaccgcc cgcaaactga cgccattaat acgcgcctgg
     6661 cctggtgaat cccagcataa acgcgccacc gttgggttgg atattctcgc tgctatcggt
     6721 agtgatatcg cccttatgca gcttaacggc atcgcccaga aactgaaatt caaagcatta
     6781 caggagcggg caaaagaaaa aattgccgac attgccgaga gccgcgaact cacggtggcg
     6841 gagcttgaag atcggttagc accggatctc ggtctggatg ataacggttc gctgctgctg
     6901 gattttggcc cacggcagtt caccgtcagc tttgatgaaa ccttaaaacc gtttgtgcgt
     6961 gatgtttccg gcagccgcct gaaagacctg cccaaaccga acaaaagcga tgatgaaacg
     7021 cgggcgaacg atgcggttaa ccgctacaaa ttgctgaaaa aagatgcgcg taccatcgcc
     7081 gcccagcagg tagcaaggct ggaatccgcc atgtgcctgc gccgccgctg gtcgctggaa
     7141 aacttccagc tcttcctggt tgagcatccg ctggttcgtc acttaacccg ccgtctgatt
     7201 tggggcgttt atagcgccga aaaccagcta ctggcttgct ttcgcgtagc agaagataac
     7261 agctacagca ccgctgacga tgatcttttc accctgccgg aaggcgatat ctctatcggc
     7321 actcctcacg ttctggaaat atcaccaacg gatgctgccg cctttggtca gctttttgcc
     7381 gactacgaac tgctaccacc gttccgccag ctcgaccgta acagctacgc cctgacagaa
     7441 gccgagcgca atgccagtga actgacccgc tgggcaggca gaaaatgccc gagtggtcgg
     7501 gtcatggggc tggcgaataa aggctggata aagggcgaac cacaggatgg aggctggatc
     7561 ggatggatga tcaaaccttt gggtcgctgg tcgttaatca tggaaatcga cgaaggtttt
     7621 gcggtgggta tgtcaccagc cgaacttagc gctgagcagc tcttaagcaa gctgtggtta
     7681 tgggaaggca aagcggaaag ctatggctgg gggagtaatt caacacagga agcgcagttc
     7741 tccgtactcg atgccatcac cgccagcgag ctaattaacg atattgaagc cctgtttgaa
     7801 taaggaaaga gcatggacaa ggaattaccg tggctggcgg ataacgccca actggaactg
     7861 aaatataaaa aaggcaaaac accactcagt catcgcaaat ggccgggcga accggtgcct
     7921 gttatcactg aaagcatcat tcagacactg ggtgatgaat tgctacaaaa agctgagaag
     7981 aaaaaaaaca ttgtctggcg ttatgaaaat ttttcactgg agtggcagtc cgccattacg
     8041 caggccatca acttgatcgg cgaacacaaa ccctcagtcc cggcccgcac aatggcggca
     8101 ctagcctgta tcgcgcaaaa tgacaaccaa cagttgctcg acgaaatcgt ccaacaagag
     8161 gggctggaat atgcgactga ggtggtgatt gcacgccagt ttattgcacg gtgttatgag
     8221 agtgatcctc tggtagtcac attgcagtat cagaacgagg actatggcta tggttatcgc
     8281 tcagaaacct ataacgaatt cgatctccga ctgcgtaaac acctctctct ggcagaggaa
     8341 agctgctggc agcgttgcgc cgacaaactc attgccgcac tgccaggaat aaccaaagtt
     8401 cgccgccctt ttattgcgct gatcctcccc gaaaaaccag aaatcgccaa tgagttggta
     8461 agccttgaat gcccgcggac tcattttcat tctaaggagt ggttaaaagt tgttgctact
     8521 gaccccaaag cggtgagaaa actcgaacgc tactggagcc aggatatatt tagcgatcga
     8581 gaagccagct acatgtcgca tgaaaaccac ttcggctacg cggcctgcgc cgcccttttg
     8641 cgcgaacaag gactggcagc cgttccgcgc ctcgcgatat atgcccataa agaagattgc
     8701 ggcagtctgc tggtacaaat taaccatccg caagtcatcc gcaccttgct gttagttgct
     8761 gataaaaaca aacccagcct gcaacgtgtg gctaaataca gtaaaaactt cccccatgcg
     8821 acactcgccg cactggcaga actgctggcg ttaaaagaac caccagcccg cccaggttat
     8881 ccaatcatcg aagacaaaaa gctgcctgca cagcaaaaag ctcgagatga atactggcgt
     8941 acgctgttac aaacgctgat agcatcgcag ccacaactag cagaaagggt gatgccgtgg
     9001 ttaagtactc aagcccagtc agtggtgaag cgttatttat cggcatcgcc aaaaccggtt
     9061 attgatagca ccgataacag cggtctgcct gaaatgctcg tttcaccacc gtggcgtagt
     9121 aagaaaaaaa tgacagctcc acgtcttgat ttggcaccgc tcgaattaac tccgcaagtt
     9181 tactggcaac caggcgaaca agagaggctt gccgccactg agtctgcccg ttatttcagc
     9241 acggaatctc ttgcggaacg catggaacaa aaaagtggac gagttgtatt acaggaactg
     9301 ggttttgggg atgatgtatg gctgtttctg aattatatac tccccggaaa actggatgct
     9361 gcacgcaatt cactcattgt tcagtggcat tactaccagg ggcgggttga agagatcctg
     9421 aatggctgga actccccgga agcacaatta gcagaacagg cgctccgcag cggtcacata
     9481 gaagcgttaa ttaacatatg ggaaaatgac aactactcac gttatcgtcc ggaaaagagt
     9541 gtctggaacc tgtatttatt ggcacagttg ccgcgtgaga tggctttgac cttctggctg
     9601 cgtatcaatg agaaaaagca tctgttcgcg ggtgaggact attttctcag tatcctcgga
     9661 ttggatgcgc taccaggtct gctgttggct ttttcacatc gtccaaaaga aacatttccg
     9721 ttaattttaa attttggcgc aacagaactg gcgctgcctg ttgcccgcgt ctggcgacgt
     9781 ttcgcggcgc agcgtgatct ggctcgccag tggattttac aatggccgga acatacggct
     9841 agtgcactta tccctcttgt ctttaccaaa cccagcgata atagcgaagc cgcattactt
     9901 gccctgcgtt tactgtacga acagggacat ggcgaattgc tacaaaccgt ggcaaaccgc
     9961 tggcagcgta cagatgtatg gtctgccctg gagcagttgc ttaaacaggg tccaatggac
    10021 atttacccgg cacgcattcc aaaagcccct gatttctggc atccggcaat gtggtccagg
    10081 cctcgactta tcactaataa tcagcctgtt accggtgacg ctctggaaat tatcggcgaa
    10141 atgctgcgct ttacccaggg gggacgtttt tatagcgggc tggaacaact gaaaacgttc
    10201 tgccagccac aaacgctggc agcttttgcc agggatctct tcactgcgtg gcaacaagct
    10261 ggtgcccctg caaaagacaa ctgggcattt ctggcgttaa gtctctttgg tgacgaaagc
    10321 acggcacggg atctgacgac acagatcctc gcctggccac aagaaggcaa atctgcccgt
    10381 gctgtcagcg gcctgaacat ccttaccctg atgaataatg atatggcgct gatacagctg
    10441 catcatatat cgcaacgggc taaatcccgc cccttacgtg ataacgcggc ggaatttctt
    10501 caggtagtcg cagaaaatcg cgggctaagc caggaagagc tagcggacag attagtccca
    10561 accctgggcc ttgatgatcc gcaggcgttg agttttgatt ttggtccccg gcagtttacc
    10621 gttcgcttcg atgaaaacct caacccggtt atctttaatc agcaaaacgt tcgccagaaa
    10681 agcgttcccc ggttgcgcgc cgatgacgat caactgaaag cgcccgaggc actggcccga
    10741 ctaaaagggc taaaaaaaga tgctactcag gtgagcaaaa acctgctacc gcgtcttgaa
    10801 gctgccctac gtaccacccg acgctggtcg ctggcagatt ttcattctct gtttgttaat
    10861 catcccttta cccgtctggt tacccagcga ttaatatggg gggtttatcc ggcaaatgaa
    10921 ccgcgttgtt tactcaaagc ctttcgtgtg gccgcagagg gggagttctg caatgcgcaa
    10981 gatgagccaa ttgacctgcc tgcggacgct ctgattggca ttgcccaccc gttagaaatg
    11041 acagtagaaa tgcgcagtga atttgcacag ctttttgccg attacgaaat tatgccgcct
    11101 tttcgccagt tgtcgcgccg cacggtgctg ctcacacctg acgagtcaac cagtaacagc
    11161 ctgactcgct gggaaggtaa atccgctacc gttgggcagc ttatgggaat gcgatacaaa
    11221 ggctgggagt caggctatga ggacgcattt gtctatgacc tgggcgagta ccggctggtc
    11281 cttaagtttt cacccggttt taaccactac aatgttgata gcaaagcgtt aatgagcttc
    11341 cgttctcttc gagtgtaccg tgacaataaa tccgtcactt ttgccgaact tgatgtgttt
    11401 gatttgagtg aggcgttaag cgcacctgac gtcattttcc attaaaaaca cagcgggcag
    11461 tgatgcaact gcccgttatc aacacgactt accagcgggg aaagcgatga tcgtgcaaaa
    11521 agagctggtt gctatttacg attatgaggt ccctgtacct gaaaatccgt tttccttcag
    11581 acttgagatc aataaatgct ctgaattatt tacaggttcc gtctatcgac tggagcgatt
    11641 ccggctacgt ccaacatttc atcaacgtga tcgagaagat gctgacccgc taataaatga
    11701 tgcgttgatt tatataagag atgagtgtat tgatgagcgg aaattacgag gtgaatcacc
    11761 tgaaactgta atagcaattt ttaatcgtga a
//
LOCUS       DAZKIV010000143        11743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZKIV010000143 DAZKIV010000000
VERSION     DAZKIV010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.143"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..193
                     /locus_tag="ACHOT6_003795"
     CDS             5..193
                     /locus_tag="ACHOT6_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948947.1"
                     /translation="MRQDGLFALAQKKMPDATLRVLYSHICQIQQRMRFPQLAYFHTC
                     PPYQKFILKLLNNHFPAN"
     gene            310..1608
                     /gene="kgtP"
                     /locus_tag="ACHOT6_003796"
     CDS             310..1608
                     /gene="kgtP"
                     /locus_tag="ACHOT6_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFP9948948.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFDVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHOT6_003797"
     CDS             complement(1605..1928)
                     /gene="yfiM"
                     /locus_tag="ACHOT6_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFP9948949.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHOT6_003798"
     CDS             complement(1974..3329)
                     /gene="pssA"
                     /locus_tag="ACHOT6_003798"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFP9948950.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHOT6_003799"
     CDS             complement(3443..6103)
                     /gene="pat"
                     /locus_tag="ACHOT6_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFP9948951.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHOT6_003800"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6135..6833)
                     /gene="tapT"
                     /locus_tag="ACHOT6_003800"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFP9948952.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHOT6_003801"
     CDS             complement(6902..7321)
                     /gene="trxC"
                     /locus_tag="ACHOT6_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFP9948953.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHOT6_003802"
     CDS             7528..8565
                     /gene="yfiF"
                     /locus_tag="ACHOT6_003802"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFP9948954.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHOT6_003803"
     CDS             complement(8613..9302)
                     /gene="ung"
                     /locus_tag="ACHOT6_003803"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFP9948955.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9607..9990
                     /gene="grcA"
                     /locus_tag="ACHOT6_003804"
     CDS             9607..9990
                     /gene="grcA"
                     /locus_tag="ACHOT6_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFP9948956.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHOT6_003805"
     CDS             complement(10046..10633)
                     /gene="eamB"
                     /locus_tag="ACHOT6_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFP9948957.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHOT6_003806"
     CDS             10736..11617
                     /gene="yfiE"
                     /locus_tag="ACHOT6_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFP9948958.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2923 a         2926 c         3037 g         2857 t
ORIGIN      
        1 ggccatccgt caggatggcc tttttgcatt ggcgcagaaa aaaatgcctg atgcgacgct
       61 gcgcgtctta tactcccaca tatgccagat tcagcaacgg atgcggtttc cccaacttgc
      121 ctacttccat acgtgtcctc cttaccagaa atttatcctt aagctcctca ataaccattt
      181 tcctgctaac taaattcatg gttaaggttg cataatgata tgcaacaaat gtataacatt
      241 ttctttacca aaagaaataa ataaaagcga ccgacaaaag catcggatta cggcaggaga
      301 cataatggca tggctgaaag tactgtaacg gcagacagca aactgacaag tagtgatact
      361 cgtcgccgca tttgggcgat tgtgggggcc tcttcaggta atctggtcga gtggttcgat
      421 ttcgatgtct actcgttctg ttcactctac tttgcccaca ttttcttccc ttccgggaac
      481 acgacgactc aactactaca aacagcaggt gtttttgctg cgggattcct gatgcgccca
      541 ataggcggtt ggctatttgg ccgcatagcc gataaacatg gtcgcaaaaa atcgatgctg
      601 ttatcggtgt gtatgatgtg tttcggatcg ctggttatcg cctgcctccc aggttatgaa
      661 actataggta cgtgggctcc ggcattattg cttctcgcgc gtttatttca gggattatct
      721 gttggcggag aatatggcac cagcgccacc tatatgagtg aagttgccgt tgaagggcgc
      781 aaaggttttt acgcatcatt tcagtatgtg acgttgatcg gcggacaact gctagcccta
      841 ctggttgtcg tggttttaca acacaccatg gaagacgctg cactcagaga gtggggatgg
      901 cgtattcctt tcgcgttagg agctgtgtta gctgttgtgg cgttgtggtt acgtcgtcag
      961 ttagatgaaa cttcgcaaca agaaacgcgc gctttaaaag aagctggatc tctgaaagga
     1021 ttatggcgca atcgccgtgc attcatcatg gttctcggtt ttaccgctgc gggctccctt
     1081 tgtttctata ccttcaccac ttatatgcag aagtatctgg taaatactgc gggaatgcat
     1141 gccaacgtgg cgagtggcat tatgactgcc gcattgtttg tattcatgct tattcaacca
     1201 ctcattggcg cgctgtcgga taagattggt cgccgtacct caatgttatg tttcggttcg
     1261 ctggcagcca tttttaccgt tcctattctc tcagcattgc aaaacgtttc ctcgccttat
     1321 gccgcttttg gtctggtgat gtgtgccctg ctgatagtga gtttttatac atcaatcagt
     1381 ggaatactga aggctgagat gttcccggca caggttcgcg cattaggcgt tggcctgtca
     1441 tatgcggtcg ctaatgctat atttggtggt tcggcggagt acgtagcgtt gtcgctgaaa
     1501 tcaataggaa tggaaacagc cttcttctgg tatgtgacct tgatggccgt ggtggcgttt
     1561 ctggtttctt tgatgctaca tcgcaaaggg aaggggatgc gtctttagtg acgggtcagt
     1621 tgccagacgg tatagccggt gcttgcaccg gcgacatccc aggccaaatc cttccagctc
     1681 cagccgctcc cttcggggcg gctatcccaa agctcttttg acgcccccaa actgacagag
     1741 aacatcaatc caaacatggc actgcgatcc cggctcatcc cctgatgctg tgaatattca
     1801 tttccggcgg cggaaagcat cgccgaggcg ataaagtgtt gagctttatc ctgcccgctc
     1861 cagctatcgt tagccatatg gctgcatcca gaaagtaaca atagcgaaca gacaaaaaga
     1921 atacgcaaaa cgtggcctcc aaaaaacaaa ccccgtacag gacggggttg tgattacagg
     1981 atgcggctaa ttaatcggtc gatgcggata cggcgcaaac ggcggatgag tttacgaacc
     2041 ttcaccggat aatcggcaat actttgcaga tcgcgatagt gcttaacgat ggtggtatgc
     2101 tcgcggatca gctccagttc tttctctcgc tgcggcgcaa gctccagttg tggatcgtgg
     2161 atcaaaatgg cgttttccag atccagacgc caggcgcgcg ggttcaggtt attaccggtg
     2221 atcaacatcc acttatcatc aacccacatc ccttttaggt gataggtgtt gtcgtcatct
     2281 ttccataacc gaaccactag ctggtcagta ttgacgtaat actgcaaacg gctcaggaaa
     2341 cgacgcagat tgatctcata gagataaggc aatgcgccaa ttatcttgaa aggttcatct
     2401 tccggaatgt agaagtcgtt cgcggtttta tcaccaacaa taatttcgac ctttttccct
     2461 tcgcgcagca actggataat attgcgcaca aggattgctg gcaggttgaa gtatggcgta
     2521 cagatggtta gtttctgctc ggcacaaggc ataagatgga aaatggtctt gttcaacaga
     2581 ctcgatttcc ccagccccac tagcggcgtt acggaaagct gatcgttgtc ggcatcgccc
     2641 tggaaatgat aagcggcatc acgcagctcc tggcggaaca gacgaatatc gttcttgatt
     2701 tccgggcttt ttggccgatt aacatcatcc agacgattaa cgccgcggcc attcataata
     2761 ttctgtgtaa cccattcaaa cataatgtct gacatcttac ggttacggat cagatgataa
     2821 cggtcgtagc gatatttatc gtgctgatgc aggtaaacat cgttcaggct ggcaccgcta
     2881 taaagtacgc tatcgtcgat gataaagcct ttaaagtgca gaacaccaag ggcttcacgg
     2941 gtattgattg gaacgccata aaccggaaca tctacgcccg gattttcctg cgccatgcgg
     3001 cagtaccagt cagcgttagt gttagatgcc gcagcgccaa tgcgtccacg ttgtgcacga
     3061 tgccagtcga ccagcacccg cacatccagt tccggacgct gccttttagc ttcatacaac
     3121 gcgttcagaa tgcctttgcc accgtcatcc tgttcgagat acagggcgac aatgcaaatg
     3181 cgctgcttcg cgctggctat tttttccagc agcgtctccc ggaagtcggc gggagcgtaa
     3241 aagaaatcga catcatcaac tgattgagaa atcttgggta gttgggcaag gtgttgttga
     3301 tgtttattac gcttaaattt tgacaacatc acagtgcatt tcttctctgt tcattgaagg
     3361 gtcctctgtg caatgcagac gacataagcg ggcaataata acaccagtcc cgattaagtg
     3421 gtcaacattt ccagtacctt actcatgatt cctcgcgttg ggcaagattt agcgtaagcc
     3481 caacgatccc ctcttcgagc tggatatcaa cgttaaaccc gagcttgcgg gctagcgcca
     3541 ccatgccacg attgtttggc atcgtaatac cattcagacg ttgtagtccg tgatctcgcg
     3601 tataggtaat caacttttcc attaagcgtc gacctaagcc taaccctttg agatccgagc
     3661 gaaccagcac agcaaattcg gcatcgatgt tatcaggatc ggaaatcgca cgcgtgacgc
     3721 cgaggatctc ttccgtttga tcaatacgtc gtaccgctac aaacgccatt tcccgatcgt
     3781 agtcgatctg tgtcatgttg gctaaatctt catgggtaaa ttcgttgatc tcgctaaagt
     3841 agcggtaata aagatcttct ttggtgactc gcgaaatgaa ttgctgaagt tgtggctcat
     3901 cttctggcaa aatcgggcgg aacaagcagc gttcaccgtt tttcaattct acccattctt
     3961 ccagctgatg cggataaggg cgcactgcca gccgactctc gttatcgcct tcaaacggcg
     4021 agatatccag cgtgacatcc agcgcggtaa attcactgcc agaagccagc aaaggatgaa
     4081 tatccagacg ctgaatttcc gggcaatcga caatcaagtt ggaaacctgc accagaagct
     4141 ggctcaagcc tgcaacatcc aatgggcgta gcgcactgcg cgcacgaatc tttttacttt
     4201 tgatcccctg aataaccaga tagcgggcca ggttcatgtt cagcggcggc agtgcgacga
     4261 cggcttgatc ttcaggacgc cactccacac cgccttcacc cagcatgatc aacggcccga
     4321 aaaccggatc gtgctcaacc acaacccgca actcctgagc gccagcacgg ttagccatac
     4381 tttgcaccaa caggccgtgg acccgcgcct gtggccaggc catttttacg cgatcgaaaa
     4441 tagcgttcgc cgcttgctgg acttcattgg ctgtacgcag gtaaagcatg acgccctgaa
     4501 cttccgattt atgtggaata tccggcgaac gcaatttcag cgccaccgga taaccaatct
     4561 gttcggcaat atgcaccgct tcggtgctat cgctggcaat ccaggtaggg agcgtgttca
     4621 tgccatacgc ttgcaggatg ggctgaactt catgggtatc gagcgacgta gccccttcgg
     4681 caatcgcctg ttgcaacaga agatgagctt ctgcggtatt ggaagtcaga ttgctgggca
     4741 acgccggcgt ttcgcgtagt tgcttctgat tacgccggta ctccaccata tgcataaaag
     4801 cagtgatggt tccttccggg gtacggtagg tcggcagccc ggcttcgctg aataaacgtc
     4861 gtgcctcttg cgaggagtgc tcgccgcacc agttcgtcag caaagagaca tatttgctgc
     4921 ggggatgatg ctttaccgct tcaattaata cttgcgcgct ttctgttgcg ggagcagcgg
     4981 cgctgggcga atgaataacc atcagcgcat caaaatcctg gctgtggagc agaatgtcca
     5041 gcgttttgac ataatgctcg ctgctggcgt catcgcgcag atcgagaggg ttagagactg
     5101 ccacatgttc tggcaatgca tcgcgcaatt tctggcaggt ttcttcgctt agcgttgcca
     5161 gcttgccatt gcgtgaccat aaggcatcca gcgccagcgc ggcaggcgca gcaccgttgc
     5221 tgataatcat caaccggtcg ccacgcagcg ggcgcatatg gctaagggtt tccaccgccg
     5281 aaaacagttc gtgggtatcc tgtacccgca acaaaccggc acgctgaata gccgcatccc
     5341 atgccgggtc cattcctgcc gtcgtgttga gcagtcgctg tgccgccggg ctacgtccgc
     5401 ttttaatcac cagaatcggt ttattacgcg aggcactacg ggctgccgaa acaaagcgtc
     5461 gcgcgtcgct taactgttcg agatagagca ggatggcgct ggttttactg tcgcgcgcca
     5521 gatagtcgag caattcatca acgtcgatat ccaggctgtc gccgagcgca ataaagtagg
     5581 aaaagcccat ctcacgctgt tgcgcccagt cgaggatggt gttggagacg gctgccgatt
     5641 gtgaaataaa cgccagcttg ccgcgtttaa tcggcacagg cgaaaagctg gcattcagac
     5701 cttgccaggg agccagtaaa cccagactgt ttggtccaag caggcgcatg ttatggcgca
     5761 gggcgcaggc gcggagatct tcgtgttgcg atgccggggc ggaaagaata atgcaggttt
     5821 tacagccttt ctcgccgagc tcttccagaa gagcaagatt acggctggca ttggtacata
     5881 aaaccgcaag gtcgggtgta aagggcaagc tggcaatatc cggccaggcc aacacaccca
     5941 acaccgcttt ccaggctggc gtcaccggga gtaccggtcc gttaaagcct cccgccagca
     6001 ggttacgcat catcaggtaa cctgcgcgat tgggtttcat cgacgcgcca attaccgcta
     6061 tcgattttgg tcgcagtagt gcttccagtc ctcgctgact cataccggtc tcccgcagaa
     6121 gtgaccgaat gattttaaac gctttctaac tgttctgctg tgatgctacc cagatgttgc
     6181 gtttttcctg ccagatagcg tgttttaaag cgggtaaaat gctcgcctaa ccctgctgcc
     6241 gccccggtat cgccggccat atctaacagt gcgatggcta cctcggcagt acaatattgg
     6301 cctgcagcct gggcttcacg caggcgatag gcagaaagcc gggaaagatc gacggaaatg
     6361 acgggaagat tatccagata cggactttta cgaaacatct tgcgagcttc cggccaggta
     6421 ccatcgagca tgataaacag cggtggctta ccggcaggtg gtgtgaagat cacttcccgt
     6481 tgctcatcag catacgaggc gggaaagacc accattggct gataatccgg gttttgtacc
     6541 agatccagca aatcctgcga gggttcggta cgcgaccatt gaaacgcaac ggtatcaggc
     6601 aaaatatcag caatcagacg cccggtatta ctgggcttca ttggctcggt gtcgaacatc
     6661 agcaaacaga agcgactttt tgcttgtgct ggggtaattg tcgaacagag acataatttc
     6721 tctggcaaaa gacagcgttg gcagcgacga acgcgattac cgcgggcaag aaaaggacgt
     6781 gttgcgcgcg caatacgctc ggcgcgtaac tggagaacag cgttttcggt cataagagag
     6841 cgtcgaaaaa acgccattgt cgcagaggag aaaacggggc acaagatgcg ccccggtaag
     6901 attaaagaga ttcgttcagc cagctatcga acggcgcttt cggtactgcg ccattaagca
     6961 tgtcgacaac ctgaccgttt ttgaaaatca tgatcgtcgg tatactacga attccaaagc
     7021 gactgctcaa ttcacgttca gcttcggtat tcactttcac aaagcgcact ttaccgctac
     7081 gctcttgcgc gacatcttca aaaattggtg cgaaattacg gcaggggccg caccacggtg
     7141 cccagaagtc gatcaccaca ggtagatcat ccttcagcaa tttgtcgagt gtttcaccgg
     7201 tcgcattaat cacctctccg tcaaacaagt cgtgaccgca gcgtccgcat tttgccgcat
     7261 cttcgatccg atcgtcggga atgcgattga tggcctgaca atgggtacaa acggtattca
     7321 taactaacct cgggatgagt aaggagactt atcggcaacg acgccggtat gtttctaata
     7381 tgttacatat tatcggggac attgttccag acaacaatct gttttattca ataggtatga
     7441 tagtcgcagg ctttggtacg aaatcatggc gaaccgcgcg gacttcgggt aatctgcgcg
     7501 cttcgcgcag cgctggtgga gaaaagcatg aacgatgaaa tgaaaggtaa aagcggcaag
     7561 gtcaaagtga tgtatgtccg cagtgatgat gattctgata aacgtaccca caacccgcgt
     7621 accgggaaag ggggcgggcg tccaggaaaa tctcgtgctg acggtggccg tcgccccgcc
     7681 cgcgatgaca aacagagtca gccccgtgac cgcaaatggg aagattcgcc gtggcgcacg
     7741 gtttcccgcg cgccgggtga tgagacgccg gaaaaggccg atcacggtgg catcagtggt
     7801 aaaagtttta ttgatccgga agtgttgcgt cgtcagcgtg cggaagaaac ccgcgtctac
     7861 ggcgaaaatg catgtcaggc acttttccag agccgtccgg aagcgattgt tcgcgcctgg
     7921 tttatccaga gtgtaacgcc gcgttttaaa gaagccttgc gctggatggc agcaaaccgc
     7981 aaagcgtacc atgtggtgga tgaagcggaa ttgacaaaag cgtcaggcac ggaacatcac
     8041 ggcggcgttt gcttcctgat caaaaagcgt aatggcacaa ccgtgcagca gtgggtaagc
     8101 caggcaggcg cgcaggattg tgtactggcg ctggaaaacg aatctaaccc gcataacctg
     8161 ggtggcatga tgcgcagctg cgcgcacttc ggcgtgaaag gcgttgtggt gcaggatgcg
     8221 gcactgctgg agtcgggggc ggctatccgt accgcagaag gcggcgcaga gcacgttcag
     8281 ccgattactg gcgacaacat tgttaacgtg ttggatgatt tccgtcaggc aggttacacc
     8341 gtagtgacca cttccagcga gcaggggaaa ccgctgttca aaaccagtct gccagcgaaa
     8401 atggtactgg tgctgggtca ggaatatgaa gggttaccgg atgccgcacg cgatccgaac
     8461 gatctgcgcg tgaagattga cggtacgggc aacgttgccg ggctgaatat ctctgttgca
     8521 accggcgttt tgcttggtga atggtggcgt cagaataaag cctgataaat cagccgggtg
     8581 gcaactctgc catccggcat tttaccgcaa atttactcac tctctgccgg taatactggc
     8641 atccagtcaa tcggcgtctc gccacgttgt tccagccact gatttgccag cacaaaatgg
     8701 ttgcagccaa agaatccacg atgcgccgaa agcggcgacg gatgcggtgc tttcagtaca
     8761 tgatggcgtt gcttatctat aatcgcccct ttcttttgcg catgtgatcc ccacaacaaa
     8821 aacaccacgc cttcgcggtg ctggttaatc aggctgatga ctttatcagt aaacgtctcc
     8881 caaccgaggc tggcgtggga atgcgcctga cctgcgcgaa ccgtcaacac agtattgagc
     8941 agcagaacgc cctgacgcgc ccagctttcg agatagccat gattagggcg ggtgaagccc
     9001 ggaatagtat tttccagctc tttatacata ttcaataatg acggaggaat ggcaatgccg
     9061 ggacgaacgg aaaatgccag accatgcgcc tgtcccggtc cgtgataagg atcctggccg
     9121 agaatcacca ctttaacgtc acccaactcg gtaaagcgga acgcgttaaa gacatctttt
     9181 tgtggtggat agatagtgac gccggactgc cgctcgctgg cgacggtctg aagggtatta
     9241 agaaaataag gttgttgctt ctcttcagcc agcacgtcat gccaggttaa ttcgttagcc
     9301 atctcgctct cctgcgaatc ttcaatccgc ctagcttaac tgcttctttc tacagaacaa
     9361 aatcatgctc attttttaga cttaaacgag tgcaaaaaaa tgtaaattgg taaaatttca
     9421 cacccgcata agttgatgta aaacaataaa atccatttac gaccacaatc gatgtgtggt
     9481 ttttattgat ttaaatcaaa gaataaaggg tggttgagga gtatatatac actcaagcaa
     9541 caatggtttt accaattggc cgcgacaggc tgaacaaatc aaataatttt gccggggagg
     9601 catcacatga ttacaggtat ccagattact aaagccgcta acgacgatct gctgaactct
     9661 ttctggctgc tggacagcga aaaaggcgaa gcacgttgca tcgttgcaaa agcaggttat
     9721 gcagaagatg aagtggttgc agtaagcaaa ctgggtgaca ttgaataccg tgaagttcca
     9781 gtagaagtga aaccagaagt tcgcgttgaa ggtggtcaac acctgaacgt taacgttctg
     9841 cgtcgtgaaa ctctggaaga tgcagttaag catccggaaa aatatccgca gctgaccatc
     9901 cgtgtatccg gttatgcagt tcgctttaac tctctgactc cggaacagca gcgcgacgtt
     9961 atcgctcgta cctttactga aagcctgtaa ggcttaagta gcaagttact aagcggaaga
    10021 gggcgacctc ttccgctttt tttcgttaat agaaaatgcg taccgcgcaa tagaccagca
    10081 acagggcaag cacgatattt aactggcgac catactggcg aaacaatcgc tgaaacagat
    10141 gccccgccag cgcccagcat acattgccaa acgtcccaat catcgccagc aaaacgctga
    10201 cgccaactac ccagcttagc gcctgtgttt gcggcagaac aaacgtcgac agtgccgtaa
    10261 caccgtacaa aatgattttg acgttcacaa actgcaaagc aaagctggcc caaaagctga
    10321 ttggttttgc ctgaagtccg tcttcctttg ttgggctggt ggcgattttc cacgccagcc
    10381 agacaatata tgccgccccc gcccaactca aaaggtgtac cgctgccggg tcaatcactg
    10441 ccagtgaaaa tgaaatgccc gcacacagta acatcacaat caaaaatccc agactcatcc
    10501 ctgccagcac gcgggtactt tgacgaaatc catgcgacgt agcagagcta agggcgagaa
    10561 tattgttcgg tcctggcgtc atagcggtaa tcagggtgta agtccaaaaa gcacttaaaa
    10621 gggtcggtgt cacttgttat ctcctctgtt ttggtagaca gtacccgaca cggaacattt
    10681 aaaaaaatga atgtttttat attctagatt cgatattttc gataggtttg gggttatgga
    10741 tctgcgccgt tttattacgc ttaaaaccgt ggtggaagag ggttcctttt tgcgagcttc
    10801 gcaaaaattg tgctgtacac aatcgacggt gacttttcat attcagcagc ttgagcagga
    10861 gttttcagtc cagttatttg agaaaattgg ccgacggatg tgccttaccc gcgaaggaaa
    10921 aaagttgctg ccgcacattt atgaacttac ccgagtgatg gatacgcttc gtgaggctgc
    10981 caaaaaggag tcagatccgg acggggaact acgcgttgtt tcgggcgaaa cgttgctctc
    11041 ttaccgaatg ccgcaggtgt tgcagcgttt tcggcaacgc gcaccaaaag tgcgtttatc
    11101 gttgcagtcg ctgaactgtt acgtgatccg tgatgcgcta ctgaatgatg aggccgatgt
    11161 cggcgtcttt tatcgcgtag ggaatgatga tgccctgaac cgacgagagt tgggtgaaca
    11221 atcgctggtg ctggtggctt caccacaaat tgtagatgtc gattttactg agccgggaag
    11281 acataacgcc tgtagcttta ttatcaatga accacaatgt gtcttccggc agatatttga
    11341 gagcacgctg cgtcagcggc ggatcacggt ggaaaacacc attgagctgt taagcattga
    11401 aagtatcaaa cgttgtgtcg cggcgaatat cggtgtcagt tacttgcccc gctttgcggt
    11461 ggaaaaagag cttgagagcg gagaattaat tgaattgcct tttggcgaac agtcgcagac
    11521 cattacggcc atgtgcgcac accatgctgg aaaagcggtt agcccggcga tgcacacttt
    11581 tattcagtgt gttgaagaga gttttgtggc tggataaaaa taatgtcggg attaaacccg
    11641 gcatttaact tgtcgacaaa gtgcgcttta ttcatgccgg atgcggcgtg aacgccttat
    11701 cctgcctaca aaagcacgca aattcaatat attgaagttt att
//
LOCUS       DAZKIV010000144        11513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZKIV010000144 DAZKIV010000000
VERSION     DAZKIV010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.144"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..356
                     /locus_tag="ACHOT6_003807"
     CDS             <1..356
                     /locus_tag="ACHOT6_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugal transfer protein TraG"
                     /protein_id="HFP9948959.1"
                     /translation="VPSGGGSQDIIADHQGHQAIIEQRTQDSNIRNDVKHQVDNMVTE
                     YKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEGKSAQERMPGADSPQ
                     ELMKRAREYQDKYKQ"
     gene            372..857
                     /locus_tag="ACHOT6_003808"
     CDS             372..857
                     /locus_tag="ACHOT6_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFP9948960.1"
                     /translation="MIMRCLTHIALVTVIQFIACYLAGWGNAETIFMLFFIVLWQGLF
                     IWLFSQIRKKRNVSDEFKFSKGVWYITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCV
                     SVREWMQSQVNEQTNEDLHLDFDNMDFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYD
                     E"
     gene            906..1637
                     /gene="traT"
                     /locus_tag="ACHOT6_003809"
     CDS             906..1637
                     /gene="traT"
                     /locus_tag="ACHOT6_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFP9948961.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1889..4042
                     /gene="traD"
                     /locus_tag="ACHOT6_003810"
     CDS             1889..4042
                     /gene="traD"
                     /locus_tag="ACHOT6_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFP9948962.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            4042..9312
                     /gene="traI"
                     /locus_tag="ACHOT6_003811"
     CDS             4042..9312
                     /gene="traI"
                     /locus_tag="ACHOT6_003811"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFP9948963.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISHQ
                     KSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTSFTELGREIDAQIKR
                     GDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDNVYHRIVGISKDDGLITLQDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDV
                     AAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQEGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREV
                     ARENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            9332..10078
                     /gene="traX"
                     /locus_tag="ACHOT6_003812"
     CDS             9332..10078
                     /gene="traX"
                     /locus_tag="ACHOT6_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFP9948964.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGSSYGIAG
                     LLMLAVSHRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVTGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
     gene            10137..10997
                     /locus_tag="ACHOT6_003813"
     CDS             10137..10997
                     /locus_tag="ACHOT6_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9948965.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            11100..11513
                     /locus_tag="ACHOT6_003814"
                     /pseudo
     CDS             11100..11513
                     /locus_tag="ACHOT6_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
BASE COUNT         2806 a         2685 c         3529 g         2493 t
ORIGIN      
        1 gcgtaccttc gggtggtggc agtcaggata ttattgctga ccatcaggga catcaggcca
       61 ttattgagca aagaacgcag gacagtaata tccgtaatga tgtaaaacat caggttgata
      121 atatggtcac agaatataaa gggaatatag gtgatactca gaacagcgta cgtggcgagg
      181 aaaatattgt aagaggacag tattctgaac tgcagaatca ccataaaaca gaggcattat
      241 ctcagaataa taaatataat gaaggaaagt cggctcagga aagaatgccc ggggctgaca
      301 gtccacagga acttatgaaa agggcaaggg aatatcagga taagtataag caataatgct
      361 gtttttggga gatgataatg agatgtttaa cccatattgc tctggtaacg gtgatacagt
      421 ttatcgcctg ttatctggct ggttggggga atgccgaaac aatttttatg ctgttcttca
      481 tagtactatg gcagggactg tttatatggt tgttctctca aatacgaaaa aaacggaatg
      541 tgtcggatga gtttaagttt agtaaaggcg tttggtatat aacgatgcca gtatcttcct
      601 tactctctcc attattaagc ctgatggttt ttatcattgg tacactatac gaattaaggc
      661 gagttagtgg ttgtgtaagc gtaagagaat ggatgcaatc tcaggtaaat gaacaaacta
      721 atgaagattt gcatctggat tttgataata tggattttca tcgtactaat cctgcaacag
      781 ggttacctat gtcaggtatt ggtgtggata tcggtggtaa ttcatatggt tatagttcaa
      841 aacgatatga tgagtgaatc ttaatttgta tattatgacg ttttattcaa tatgaaggaa
      901 cattgatgaa aatgaaaaaa ttgatgatgg ttgcactggt cagttccact ctggcccttt
      961 cagggtgtgg tgcgatgagc acagcaatca agaagcgtaa ccttgaggtg aagactcaga
     1021 tgagtgagac catctggctt gaacccgcca gcgaacgcac ggtatttctg cagatcaaaa
     1081 acacgtctga taaagacatg agtgggctgc agggcaaaat tgctgatgct gtgaaagcaa
     1141 aaggatatca ggtggtgact tctccggata aagcctacta ctggattcag gcgaatgtgc
     1201 tgaaggctga taagatggat ctgcgggagt ctcagggatg gctgaaccgt ggttatgaag
     1261 gcgcagcagt tggtgcagcg ttaggtgccg gtatcaccgg atataattcc agttctgccg
     1321 gtgccacact cggtgtgggc cttgctgccg gtctggtggg tatggctgca gatgcgatgg
     1381 tggaagatgt gaactatacc atgatcacgg atgtgcagat tgcagagcgt actaaggcaa
     1441 cggtgacaac ggataatgtt gccgccctgc gtcagggcac atcaggtgcg aaaattcaga
     1501 ccagtactga aacaggtaac cagcataaat accagacccg tgtggtctca aatgcgaaca
     1561 aggttaacct gaaatttgaa gaggcgaagc ctgttctcga agaccagctg gccaaatcaa
     1621 tcgcaaatat tctctgatta tcttccggag gctggtctga ccggcctcct gactgacgcc
     1681 tcgctttgct cgttgtccaa ccccggaaag aaataaaata aattttattt ctccccggcg
     1741 tttccgaaat aaatatgaaa acactgagcg gttgtgcaaa ttctttgtgc gaccgtgaag
     1801 tgttgccggc gaagccggaa tttaacggtt attaatcttt tcatcattaa taatcagtcc
     1861 gactgacttt tttcttcgga atatcatcat gagttttaac gcaaaggata tgacccaggg
     1921 cggtcagatt gcgtccatgc gtatccgcat gttcagccag atcgccaata tcatgcttta
     1981 ctgcctgttt atttttttct ggatactcgt tggtctggtt ttatgggtaa aaataagctg
     2041 gcagacgttt gtgaacggct gtatttactg gtggtgtacc acgctggaag gcatgcggga
     2101 tttaatcaag tcccagccgg tatatgagat ccagtattac ggcaaaacct tccggatgaa
     2161 tgctgctcag gtactgcatg ataaatatat gatctggtgc ggagagcagc tatggtccgc
     2221 attcgttctg gccacagttg tggcactggt tatttgcctg attaccttct ttgttgtctc
     2281 ctggattctg gggcgtcagg gtaaacaaca gagcgaaaat gaagtcacag gtggtcgtca
     2341 gctgacggag aacccgaaag acgttgcccg gatgctgaaa aaagacggca aggactccga
     2401 tatacggatt ggcgacctgc cgattatccg ggattccgaa atccagaact tctgtctgca
     2461 cggcacggtc ggggccggta agtcggaagt tatccgtcgt ctggccaact acgcccgtca
     2521 gcgtggagat atggtggtga tttatgaccg ttcaggggaa tttgttaaaa gttactatga
     2581 tccctccatc gataaaatcc tgaatccgct ggatgcccgc tgtgccgcct gggatttgtg
     2641 gaaggagtgc ctgacacagc cggattttga taataccgcg aacacgctga tcccgatggg
     2701 cacaaaagag gacccgttct ggcagggttc aggacgtacc atttttgcgg aagcggcgta
     2761 cctgatgcgt aatgacccca accgcagcta cagcaaactg gtggacacac tgctttccat
     2821 caaaatcgaa aaactgcgta ccttcctgcg taattcaccg gcggccaacc tggtggaaga
     2881 gaaaattgag aaaacggcta tttccatccg tgctgtgctg accaactacg tgaaagccat
     2941 ccgttacctg caggggattg agcataacgg tgagtctttt accatccgtg actggatgcg
     3001 gggtgtccgg gaagatcaga aaaacggctg gctgtttatc tcgtcgaatg ccgacaccca
     3061 tgcctccctg aagccggtga tctccatgtg gctgtccatt gccattcgtg gtctgctggc
     3121 aatgggagaa aaccgtaacc gtcgtgtgtg gtttttctgt gatgagctac ccacgttaca
     3181 caaactgccg gacctggtgg agatcctgcc ggaagcccgt aagttcggtg gctgttatgt
     3241 gtttggtatc cagtcctatg cccagctgga agatatctac ggtgagaaag cggctgccac
     3301 gctgtttgac gtcatgaaca cccgtgcctt tttccgttct cccagccata agattgcaga
     3361 gttcgctgcc ggtgagattg gtgagaaaga gcacctgaaa gccagcgagc agtattccta
     3421 cggtgctgat ccggtacgtg acggggtatc gaccggtaag gatatggagc gccagacgct
     3481 ggtcagttat tccgacattc agtctctgcc ggatctgacc tgttatgtca ccttgcccgg
     3541 accgtatccg gcagtaaaac tctcactgaa atatcaggca cgtccgaagg ttgcccctga
     3601 gtttattccg cgtgacatca acccggaaat ggagaaccgt ctgagtgccg tacttgccgc
     3661 aagggaagca gaaggtcgtc agatggccag cctcttcgaa ccggatgtgc cggaagttgt
     3721 ttccggagaa gacgtgactc aggctgaaca gccgcaacag ccggtgtctc ctgccatcaa
     3781 cgataagaag tcagattcag gtgtgaatgt tccggcaggg gggatcgagc aggagctgaa
     3841 aatgaaaccg gaagaagaga tggaacagca actgccaccc gggatcagtg aatccggtga
     3901 agtggtggat atggccgctt atgaggcatg gcaacaggaa aatcatccgg acatccagca
     3961 gcagatgcag cgtcgtgaag aggtgaacat taatgtgcac cgggagcgtg gggaggatgt
     4021 tgagccggga gatgatttct gatgatgagt atcgcgcagg tcagatcggc cggaagtgcc
     4081 ggtaactatt ataccgacaa ggataattac tatgtactgg gcagcatggg agaacgctgg
     4141 gccggcaggg gggctgaaca gctgggactg cagggcagtg tcgataagga tgtttttacc
     4201 cgtcttctgg agggaaggct gccggacgga gcggatctaa gccgcatgca ggatggcagt
     4261 aataagcatc gtcccggcta cgacctgacc ttctccgccc ccaaaagtgt ctccatgatg
     4321 gccatgctcg ggggggacaa acgtcttatt gaagcacata accaggccgt ggactttgct
     4381 gttcgtcagg tggaggcgct ggcctccaca cgggtgatga cggacggaca gtcagaaacg
     4441 gtactgaccg gtaatctggt gatggcactg tttaaccacg acaccagtcg cgatcaggaa
     4501 ccacagttac acacgcatgc ggtggtggct aatgtcacgc agcataacgg tgagtggaag
     4561 acactgagca gtgacaaagt ggggaaaacg gggttcattg agaatgtgta cgctaatcag
     4621 attgcctttg gcaggctcta ccgggaaaaa ctgaaagagc aggttgaggc gctgggctat
     4681 gaaaccgaag tggtgggtaa gcacggtatg tgggagatgc cgggcgtacc ggtggaggcc
     4741 ttttccggac gctcacagac tatccgggag gccgtcgggg aagatgcctc gctgaaatcc
     4801 cgggatgtgg cggcactgga tacgcgaaaa tccaaacagc acgtcgatcc tgaagtcaga
     4861 atggctgaat ggatgcagac gctgaaggaa accgggttcg acatccgggc atatcgtgac
     4921 gcggcggatc agcgtgcgga gacccgcacg caggcgcccg gggctgtttc acaggagggg
     4981 ccggatgtgc agcaggcggt gacacaggcg attgccggat taagtgaacg aaaagtgcag
     5041 tttacgtaca cggacgtgct ggccagaacg gtcggtatac tgccgccgga aaatggtgtg
     5101 attgaacggg cacgcgccgg tatcgatgag gccatcagtc gtgagcagct tatccccctc
     5161 gaccgtgaga aggggctgtt cacatcagga attcatgtgc tcgatgagct gtccgtccgg
     5221 gcactcagtc gtgacatcat gaaacagaac cgggtgaccg tacatccgga gaaaagtgtc
     5281 ccccggacgg ctggttacag cgatgcggtc agtgttctgg cacaggaccg tccgtcgctg
     5341 gccattgtgt ccgggcaggg cggtgcagcc gggcagcgtg agcgggtggc tgaactggtc
     5401 atgatggccc gggagcaggg gcgggaggtg cagattatcg ctgctgaccg tcgctcgcag
     5461 atgaacctga agcaggatga acggttgtcc ggtgagctga taaccggacg tcgtcagctg
     5521 ctggaaggca tggccttcac gccgggcagt actgttatcg ttgaccaggg cgaaaaactc
     5581 tccctgaaag agacgttaac cctgctggac ggtgccgcac gtcataacgt acaggtcctg
     5641 ataaccgaca gcgggcagcg aaccggtaca ggcagtgcac tgatggccat gaaggatgcc
     5701 ggggtgaaca catatcgctg gcagggggga gaacagcgac cggccaccat catcagtgaa
     5761 ccagaccgta atgtccgcta tgcccggctg gccggagatt ttgcggccag cgtgaaagcc
     5821 ggagaagaga gcgtggcaca ggtcagcggg gtacgggaac aggccatact gacacaggcc
     5881 attcgcagtg agctgaaaac acagggcgtg ctcggacatc aggaggtgac catgacggca
     5941 ctgtcaccgg tctggctgga tagccggagc cgttatctgc gggatatgta ccgcccggga
     6001 atggtgatgg agcagtggaa tccggagaca cgcagtcatg accgctatgt gatagaccgg
     6061 gtgacggcac agagtcacag cctgaccctg cgggatgcgc agggggaaac gcaggtggtg
     6121 cgtatttcct ccctggacag cagctggtcg ctgttccggc cggaaaaaat gccggtggca
     6181 gacggcgagc gactgagggt gacagggaaa attcccggac tccgcgtctc cggcggtgac
     6241 cgcctgcagg tggcatccgt cagtgaagat gcgatgacgg ttgttgtgcc ggggcgggct
     6301 gaaccggcca ccctgcctgt ggctgattca ccgttcacgg cactgaagct ggagaacggc
     6361 tgggtggaaa cgcccgggca ttccgtcagt gacagtgcga aggtttttgc ctccgtcaca
     6421 cagatggcaa tggacaacgc caccctgaac ggtctggccc gcagcggtcg tgatgtccgg
     6481 ctgtattcct cactggatga aacccgtact gcggaaaaac ttgcccgcca tccctccttt
     6541 acggtggttt ctgagcagat aaaggcgcgt gccggtgaga catcgctgga aacggctatc
     6601 agtcatcaga agtccgcact tcacacgccg gcacagcagg ccattcatct ggcccttccg
     6661 gtggtggaaa gtaaaaactt ggccttcagc catgtggacc tgctgacaga ggcaaagtcg
     6721 tttgctgcag aaggaaccag ttttactgaa ctggggcggg aaatcgatgc gcagataaaa
     6781 cggggtgatt tactgcatgt ggatgtggct aaaggctatg gcacagacct gctggtttcc
     6841 cgtgcgtcgt atgaggcaga aaagagcatt cttcgccata ttctcgaggg taaggaggcg
     6901 gtcacgccgc tgatggagag agtacccggc gaactcatgg agacgttaac gtcgggacag
     6961 cgtgccgcca cccgtatgat actggaaacg tccgaccgtt tcacggtggt acagggttat
     7021 gccggtgtgg gtaagaccac acagttccgg gcggtgatgt cagccgtgaa catgctgtcg
     7081 gcgagtgagc gtccccgagt tgtggggctg gggcccacgc accgtgcggt cggtgagatg
     7141 cgcagcgctg gcgtggatgc acagacgctg gcgtcctttc tgcatgacac gcagctgcag
     7201 cagcgcagcg gagaaacgcc ggatttcagc aacacgctgt ttctgctcga tgagagctca
     7261 atggtgggca ataccgacat ggcacgggca tacgccctga ttgcggccgg tggcggtcgt
     7321 gctgtggcca gcggtgacac ggaccagctg caggccatcg cgcccggtca gcctttccgt
     7381 ctccagcaga cgcgcagtgc tgccgatgtg gccatcatga aagagattgt gcgtcagacg
     7441 ccggaactgc gggaggcggt atacagcctg attaaccggg atgtggaaag ggcactgtcc
     7501 gggcttgaga gtgtgaaacc gtctcaggtg ccacgtctgg agggcgcatg ggcaccggag
     7561 cactccgtga cggagttcag tcacagccag gaagcgaaac tggcagaagc gcagcagaag
     7621 gcgatgctga aaggcgaggc tttcccggat gtccccatga cactgtatga agccattgtc
     7681 cgcgactata ccggcagaac accggaagca cgggagcaga cgctgattgt cacgcacctg
     7741 aatgaggacc ggcgcgtgct gaacagcatg attcatgatg cacgggaaaa ggccggtgag
     7801 ctgggaaaag agcaggtcat ggtgcctgtc ctgaacacag cgaatatacg cgacggggag
     7861 ctgcgtcgtc tctccacctg ggagaataac ccggatgcac ttgccctggt ggataatgtg
     7921 tatcaccgga ttgtcggtat cagcaaggat gacgggctga taaccctgca ggatgcggaa
     7981 ggtaacacgc ggctgatttc gccccgggag gcggtggctg aaggcgtcac actgtacacc
     8041 ccggacacca tccgggtggg gaccggtgac cggatgcgct tcacgaagag tgaccgggag
     8101 cgcggttatg tggccaacag cgtctggacg gtgacagcag tttccggtga cagtgtcacg
     8161 ctgtcggacg gacagcagac ccgggtgatt cgccccggcc aggagcaggc agagcaacat
     8221 attgacctgg cctatgccat caccgctcac ggtgcgcagg gggcgagtga aacctttgcc
     8281 atcgcgcttg aaggcacgga aggtaaccgg aaacagatgg ccggctttga gtcagcctac
     8341 gtggccctgt cgcgtatgaa gcagcatgtg caggtgtaca ccgataaccg tcagggctgg
     8401 acggatgcca ttaacaatgc cgtacagaaa ggaactgccc acgatgtgct ggagccgaaa
     8461 ccggaccggg aggtcatgaa tgcgcagcgg ctgttcagta cggcacggga actgcgggac
     8521 gtggcggcag gccgtgccgt tctccgtcag gcggggctgg ccgggggaga cagtcctgca
     8581 cggtttattg ctccgggacg taaatatccg caaccgtatg tggcactgcc ggcttttgac
     8641 cgtaacggca ggtccgccgg tatctggctg aacccgctga ccacggatga cggaaacggg
     8701 ctgcggggat tcagcggtga aggtcgggta aaaggcagcg gggatgcgca gttcgtggcc
     8761 ctgcagggca gccgtaacgg agaaagcctg ctggctgata atatgcagga gggtgtccgg
     8821 attgcccgtg ataatcctga cagcggtgtg gtggtgagaa tcgccggtga aggccgtccg
     8881 tggaatcccg gtgccatcac cggtggtcgc gtgtgggggg atatcccgga caacagcgtc
     8941 cagccggggg ccggaaatgg tgaaccggtt acagctgaag tgctggcaca gcggcaggct
     9001 gaagaggcca tccgccgtga aacggaacgt cgcgcagatg aaattgtccg taaaatggca
     9061 gagaacaaac ctgacctgcc ggatggcaga acagagcagg ctgtcaggga gattgccggg
     9121 caggagcgtg accgggctgc cataactgaa cgggaagccg cgctgccgga gagtgtgctg
     9181 cgtgaatcac aacgggagcg ggaagcggtc cgggaggttg cccgggaaaa tctgctgcag
     9241 gagcgtctgc agcagatgga gcgggatatg gtccgtgacc tgcagaaaga gaaaaccctg
     9301 ggcggagact gatacaggaa gacaaacact gatgacaacg gataacacga atacgacacg
     9361 taacgattca ctggctgcac ggaccgatac ctggttgcag tcgtttctgg tctggtcacc
     9421 cggacagcgg gatatcatca aaacggtggc actggtgctg atggtactgg accacatcaa
     9481 tctgatattc cagctgaagc aggaatggat gtttctggcc gggcgaggag cctttccgct
     9541 gtttgccctg gtgtgggggc tgaatctgtc ccgtcatgcg catatccggc aaccggccat
     9601 taaccggctg tggggatggg gaattattgc ccagttcgca tattatctgg ccggctttcc
     9661 ctggtatgag gggaatatcc tgtttgcctt tgcagtggcg gcacaggtgc tgacgtggtg
     9721 tgaaacgcgc agtgggtggc gtacagcagc ggccattctt ctgatggccc tgtgggggcc
     9781 tttgtccggc tccagttacg gcattgccgg gctgctgatg ctggcagtca gtcaccggct
     9841 gtaccgggcg gaagacagag cggaacgtct ggcgctggtc gcctgcctgc tggctgttat
     9901 tccggcgctt aaccttgcca ccagtgatgc agcggcggtg accggtcttg tgatgacggt
     9961 gctgaccgtt ggtctggtgt cgtgtgcagg aaaatcatta ccccgtttct ggcccgggga
    10021 ttttttcccg gtattctacg cctgtcatct ggctgtgctg ggcgttctgg cgttgtgact
    10081 ggtatgatac tgacgctgat tctgattatt aataaacaac ataacacagg atatttatga
    10141 aaattacgga tcacaaactc tctgaaggta ttgccctgac atttcgtgtc ccggaaggaa
    10201 atataaaaca tcctttgatt ctcttatgcc atggattttg cggtatccgg aatgttctgc
    10261 tgccttcctt tgcgaatgcg tttacagaag ccgggtttgc aacgattact tttgattatc
    10321 gtggattcgg tgagagtgaa ggggaacgag gtcgtctggt tccggcaatg cagaccgaag
    10381 atattatttc agttatcaat tgggcagaaa agcaggtatg tattgataat cagcgtatag
    10441 gattatgggg aacatcgctg ggtgggtgcc atgtattcaa tgcagctgcg caggataaaa
    10501 gagtgaaaag tattgtcagc cagctggctt ttgctgacgg tgaggttctg gttaccggag
    10561 aaatgaatga actggagaaa gcttcttttc tgtccacgct gaataaaatg gcagaaaaga
    10621 agaaaaatac cgggaaagag atgtttgtgg gggttacccg ggtactgagt gataatgaat
    10681 caaaggtatt cttcgagaag gtaaaaggtc agtatcctga aatggatata aaaatacctt
    10741 tccttacggt tatggagacg ttgcaatata aaccggcaga gagcgcagcc aaagtacagt
    10801 gcccagtttt ggttgttatt gccggccagg atagtgttaa tccaccagaa cagggaagag
    10861 ccttatatga tgctgttgct tctggtacta aggaattata cgaggaagct gacgcatgcc
    10921 attatgatat ttatgaaggc gcgttttttg aacgtgtggc ggcagtacag acgcaatggt
    10981 ttaaaaaaca tctttaattg tcggtattaa tatgtcagag gtcgttgagg tcagcttaat
    11041 atcatagtga gtagtttcaa cgacctggag gacatatttt cacaaaggtc agggcaatca
    11101 tgacagagca gaaacgaccg gtactgacac tgaaacggaa aacggaaggg gaaacaccga
    11161 cccggagccg gaaaaccatc atcaatgtca ccacgccacc aaaatggaag gtgaaaaagc
    11221 agaaactggc ggagaaggct gcccgggaag ggaagcagag ctgacagcaa aaaaagcgca
    11281 ggccagacag gcgctgtcca tttatctgaa cctgccctcg ctggatgagg ccgtgaacac
    11341 cctgaaaccc tggtggccgg gattatttga cggtgacaca ccccggcttc tggcctgcgg
    11401 tatccgggac gtgttactgg aagacgtggc gcagcggaat atcccgctct cgcataaaaa
    11461 actgcgcagg gcgctgaagg ccatcacccg ttcagaaagc tatctgtgtg cca
//
LOCUS       DAZKIV010000145        11482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZKIV010000145 DAZKIV010000000
VERSION     DAZKIV010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.145"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            308..607
                     /gene="yoaC"
                     /locus_tag="ACHOT6_003815"
     CDS             308..607
                     /gene="yoaC"
                     /locus_tag="ACHOT6_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFP9948966.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMESGERTVIKNPEYFSTYMQEELKALV"
     gene            complement(727..906)
                     /gene="yoaH"
                     /locus_tag="ACHOT6_003816"
     CDS             complement(727..906)
                     /gene="yoaH"
                     /locus_tag="ACHOT6_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFP9948967.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            980..2341
                     /gene="pabB"
                     /locus_tag="ACHOT6_003817"
     CDS             980..2341
                     /gene="pabB"
                     /locus_tag="ACHOT6_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFP9948968.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2345..2923
                     /gene="nudL"
                     /locus_tag="ACHOT6_003818"
     CDS             2345..2923
                     /gene="nudL"
                     /locus_tag="ACHOT6_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFP9948969.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPSDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3107..4471
                     /gene="sdaA"
                     /locus_tag="ACHOT6_003819"
     CDS             3107..4471
                     /gene="sdaA"
                     /locus_tag="ACHOT6_003819"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFP9948970.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4602..6200
                     /gene="pdeD"
                     /locus_tag="ACHOT6_003820"
     CDS             4602..6200
                     /gene="pdeD"
                     /locus_tag="ACHOT6_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9948971.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6204..7760)
                     /gene="yoaE"
                     /locus_tag="ACHOT6_003821"
     CDS             complement(6204..7760)
                     /gene="yoaE"
                     /locus_tag="ACHOT6_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFP9948972.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7748..7906)
                     /gene="yoaL"
                     /locus_tag="ACHOT6_003822"
     CDS             complement(7748..7906)
                     /gene="yoaL"
                     /locus_tag="ACHOT6_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFP9948973.1"
                     /translation="MDRHRRHFSIRPFNAYLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8223..9194
                     /gene="manX"
                     /locus_tag="ACHOT6_003823"
     CDS             8223..9194
                     /gene="manX"
                     /locus_tag="ACHOT6_003823"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFP9948974.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9257..10057
                     /gene="manY"
                     /locus_tag="ACHOT6_003824"
     CDS             9257..10057
                     /gene="manY"
                     /locus_tag="ACHOT6_003824"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFP9948975.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10070..10921
                     /gene="manZ"
                     /locus_tag="ACHOT6_003825"
     CDS             10070..10921
                     /gene="manZ"
                     /locus_tag="ACHOT6_003825"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFP9948976.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10976..11434
                     /gene="yobD"
                     /locus_tag="ACHOT6_003826"
     CDS             10976..11434
                     /gene="yobD"
                     /locus_tag="ACHOT6_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFP9948977.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2827 a         2886 c         2874 g         2895 t
ORIGIN      
        1 tatttttata atttcaattt tagctataaa gttaactata taaataaaca taaccatgcc
       61 gttgtgttcg cccttcgatt agcacctctg gttttctttc ttagctgaac aatctggtta
      121 tagcgtgcgg actgaccgtt aattttctcc atcttactgg ataacacacc atgagctgct
      181 acctttaaaa cacctgaatg tcaacaggtt aactcgtgcc atatcgttat aatccctttg
      241 atattagatg caaattaagg tcatatatag ccttatcgat aacatggtta attttaagga
      301 taagaatatg cctgctgtaa tagataaagc cctggatttc attggtgcca tggatgtatc
      361 agcgccaaca ccaagttcga tgaatgaaag cacagcgaag ggaatcttta aatatttaaa
      421 agaactggga gtacccgcga gtgccgctga tattacggcg cgagctgacc aggaaggctg
      481 gaaccctggg ttcacggaaa aaatggttgg atgggcaaaa aaaatggagt ctggtgaacg
      541 cactgtaatt aaaaatcctg aatacttttc aacatatatg caggaagaac taaaagcact
      601 ggtctgagct taatttatat cagcataaat gggtcaggac gcttttaatc acatataaaa
      661 agcgtccttt ttctcacaat caaataatca tcggattcta taacgaaatc gatacggatt
      721 agtgtgttac tcgtcttcat cctcaaaacg cgccacgatt cgctcgccgg agtggttggc
      781 acgcagctct tctgccacca gcgcgattgc ctggccgctg ctcatccctt gtgccatcag
      841 ttcctggatc cgctcgacag ctttttgctg ctgttcatgg gtgagtgaag gtaaacctgc
      901 aaacattgtt aactcctgct aaattgttgg cgctaattat ttcatgctac ccggcacata
      961 gccagtagag tcaggactga tgaagacgtt atctcccgct gtgattactt taccctggcg
     1021 tcaggacgcc gctgaatttt atttctcccg cttaagccac ctgccgtggg cgatgctttt
     1081 acactccggc tatgccgatc atccgtatag ccgctttgat attgtggtcg ccgatccgat
     1141 ttgcacttta accactttcg gtaaagaaac cgttgttagt gaaagcgaaa aacgcacaac
     1201 gaccactgat gacccgctac aggtgctcca gcaggtgctg gatcgcgcag acattcgccc
     1261 aacgcataac gaagatttgc catttcaggg cggcgcactg gggttgtttg gctacgatct
     1321 gggccgccgt tttgagtcac tgccagaaat tgcggaacaa gatatcgttc tgccggatat
     1381 ggcagtgggt atctacgatt gggcgctcat tgtcgaccac cagcgtcata cagtttcttt
     1441 gctgagtcat aatgatgtca atgcccgtcg ggcctggctg gaaagccagc aattctcgcc
     1501 gcaggaagat ttcacgctca cttccgactg gcaatccaat atgacccgcg agcagtacgg
     1561 cgaaaaattt cgccaggtac aggaatatct gcacagcggt gattgctatc aggtgaatct
     1621 cgcccagcgt tttcatgcga cctattctgg cgatgaatgg caggcattcc ttcagcttaa
     1681 tcaggccaac cgcgcgccat ttagcgcttt tttacgtctt gaacagggtg caattttaag
     1741 cctttcgcca gagcggttta ttctttgtga taatagtgaa atccagaccc gcccgattaa
     1801 aggcacgcta ccacgcctgc ccgatcctca ggaagatagc aaacaagcag aaaaactggc
     1861 gaactcagcg aaagatcgtg ccgaaaatct gatgattgtc gatttaatgc gtaatgatat
     1921 cggtcgtgtt gccgtagccg gttcggtaaa agtaccagag ctgttcgtgg tggaaccctt
     1981 ccctgccgtg catcatctgg tcagcactat aacggcgcga ctaccagaac agttacacgc
     2041 cagcgatctg ctgcgcgcag cttttcctgg tggctcaata accggggctc cgaaagtacg
     2101 ggctatggaa attatcgacg aactggaacc gcagcgacgt aatgcctggt gcggcagcat
     2161 tggctatttg agcttttgcg gcaacatgga taccagcatt actatccgca cgctgactgc
     2221 cattaacgga caaatatact gctctgcggg cggtggaatt gtcgccgata gccaggaaga
     2281 agcggaatat caggaaactt ttgataaagt taataagata ttacgccaac tggagaagta
     2341 agacgtggaa taccgtagcc tgacgcttga tgatttttta tctcgctttc aacttttgcg
     2401 cccgcaaatt aaccgggaaa ccctaaatca tcgtcaggct gccgtgttaa tccccatcgt
     2461 ccgtcgaccg caaccggggt tgttgctgac tcagcgttca attcatctgc gtaaacacgc
     2521 tggacaagtg gcattccctg gaggtgcagt cgatgacacg gacgcatcag ttatcgccgc
     2581 cgcgctgcgc gaagctgaag aagaggtcgc tataccgcct tccgccgttg aagttatcgg
     2641 cgtgctgccg cccgtcgata gcgtaactgg ctatcaggta accccagtgg tcggcattat
     2701 cccgtccgat ctgccgtatc gcgccagtga agatgaagtc tcggcggtgt ttgaaatgcc
     2761 gctcgcccag gcattacatc tgggtcgtta tcacccttta gatatctacc gccgtggcga
     2821 ttcacatcgg gtatggctgt cctggtacga acagtatttt gtatggggaa tgaccgcagg
     2881 cataattcgt gagctggcgc tgcaaattgg tgtgaaaccc tgactatact tatctttaca
     2941 tctacaaaac actacttgag acaatcatcg caatattagt taaatcgcgg tttttgatta
     3001 gtttaattca tgtgaatagt taagccagtc gccgcgttcc ctcttacact atgcgctgtt
     3061 attagttcgt tactggaagt ccagtcacct tgtcaggagt attatcgtga ttagtctatt
     3121 cgacatgttt aaggtgggga ttggtccctc atcttcccat accgtagggc ctatgaaggc
     3181 gggtaaacag ttcgtcgatg atctggtcga aaaaggctta ctggatagcg ttactcgcgt
     3241 tgccgtggac gtttatggtt cactgtcgct gacgggtaaa ggccaccaca ccgatatcgc
     3301 cattattatg ggtcttgcag gtaacgaacc tgccaccgtg gatatcgaca gtattcccgg
     3361 ttttattcgc gacgtagaag agcgcgaacg tctgctgctg gcacagggac ggcatgaagt
     3421 ggatttcccg cgcgacaacg ggatgcgttt tcataacggc aacctgccgc tgcatgaaaa
     3481 cggtatgcaa atccacgcct ataacggcga tgaagtcgtc tacagcaaaa cttattattc
     3541 catcggcggc ggttttatcg tcgatgaaga acactttggt caggatgctg ccaacgaagt
     3601 gagcgtgccg tatccgttca aatctgccac cgaactgctc gcgtactgta atgaaaccgg
     3661 ctattcgctg tctggtctcg ctatgcagaa cgaactggcg ctgcacagca agaaagagat
     3721 cgacgagtat ttcgcgcatg tctggcaaac catgcaggca tgtatcgatc gcgggatgaa
     3781 caccgaaggt gtactgccag gcccgctgcg cgtgccacgt cgtgcgtctg ccctgcgccg
     3841 gatgttagtt tccagcgata aactgtctaa cgatccgatg aatgtcattg actgggtaaa
     3901 catgtttgcg ctggcagtta acgaagaaaa cgccgccggt ggtcgtgtgg taactgcgcc
     3961 aaccaacggt gcctgcggta tcgttccggc agtgctggct tactatgacc actttattga
     4021 atcggtcagc ccggacatct atacccgtta ctttatggca gcgggcgcga ttggtgcatt
     4081 gtataaaatg aacgcctcta tttccggtgc ggaagttggt tgccagggcg aagtgggtgt
     4141 tgcctgttca atggctgctg cgggtcttgc agaactgctg ggcggtagcc cggaacaggt
     4201 ttgcgtggcg gcggaaattg gcatggaaca caaccttggt ttaacctgcg acccggttgc
     4261 agggcaggtt caggtgccgt gcattgagcg taatgccatt gcctctgtga aggcgattaa
     4321 cgccgcacgg atggctctgc gccgcaccag tgcaccgcgc gtctcgctgg ataaggtcat
     4381 cgaaacgatg tacgaaaccg gtaaggacat gaacgccaaa taccgcgaaa cctcacgcgg
     4441 tggtctggca atcaaagtcc agtgtgacta atacttctta ctcgcccatc tgcaacggat
     4501 gggcgaattt atacccgctt tctcgtctgc tgtaatattc cccactacac ttccactgtt
     4561 gcgtcaggcg tttgtcgcca tacgcttaca gggtggcccg catgcaaaaa gcacaacgga
     4621 tcattaaaac ctatcgccgt aatcgaatga ttgtttgtac gatttgcgca ctcgttacgc
     4681 tcgcttcgac cctgagcgtg cgatttattt cacagcgtaa cttaaatcaa caacgggtag
     4741 tacaattcgc caatcacgct gtagaggaat tagataaagt actgcttccc ctacaggcag
     4801 gtagcgaagt cttgcttccg ctgattggtc tgccctgctc tgtcgcccat ttgccattac
     4861 gtaaacaggc ggcaaaactc caaactgtgc gatccattgg cctggtgcaa gacggcacac
     4921 tctattgctc cagcattttt ggttatcgca atgtgcccgt cgtggacatt ctggctgaac
     4981 ttcctgcacc gcaaccactt ttacgcctga cgatcgaccg tgccctgatt aaaggcagtc
     5041 cggttttgat tcaatggacg cctgcagcgg gcagtagcaa tgctggggtc atggagatga
     5101 ttaacatcga cttactgacg gcaatgctcc ttgagccaca actgccgcaa atcagtagcg
     5161 ccagcctgac ggtggacaaa cggcatttgc tctatggtaa tgggctggta gattcccttc
     5221 cgcaacctga agacaatgaa aactatcagg tttcttcgca acgctttcct tttaccatta
     5281 acgttaatgg tccgggggct acggcgctgg catggcacta tcttccaaca caattaccgc
     5341 tggcggtgct gctaagttta ctggtgggct acatcgcctg gctggcgacc gcttaccgga
     5401 tgagcttttc ccgcgaaatc aatctgggcc tggcgcaaca tgagttcgaa ttgttctgtc
     5461 agcctttgct taatgcgcgc agccagcaat gtattggtgt agagattttg ctgcgctgga
     5521 acaatccgcg tcagggctgg atttcaccgg atgtgtttat tcctatcgcg gaagaacatc
     5581 atttaattgt gccactgacc cgctatgtga tggcagaaac cattcgtcag cgccatgttt
     5641 tcccgatgag tagtcagttt catgttggca ttaacgtcgc acccagccat tttcgccgtg
     5701 gtgtgctgat aaaagatctc aatcagtact ggtttagcgc tcacccgatt cagcaactga
     5761 tcctcgaaat caccgaacgc gatgccttac tggatgttga ttatcggatt gcccgcgagc
     5821 tacatcgtaa aaacgtcaaa ctggcgattg atgacttcgg caccggcaac agttcatttt
     5881 cctggcttga aacattacgt cctgacgtgc tgaaaattga taagtcattt accgcagcta
     5941 taggttctga cgcggttaac tcgacggtga ccgatatcat catcgcgctg gggcaaagac
     6001 tgaatattga actggtggcg gagggcgtgg aaacgcaaga acaggcgaag tatttgcgcc
     6061 gtcatggcgt gcatattttg caagggtatt tgtacgcaca gccgatgccg ctacgtgatt
     6121 ttcccaaatg gctggcgggc agccaaccgc cgcccgcccg gcataatgga catatcacgc
     6181 ccattatgcc gttacgttaa tgcttactca tcttcatcgt gcgccggttg ctctttaaca
     6241 atgcgaacca gatcaacacg ataatcattg gcttcaatga tggtgatatg cagtggccct
     6301 acatcaatca catcgcccac acggggaatg tgaccatttg ccgagatcac gaggcccgcg
     6361 accgtcgcga tatcatcgtc atcggcaagg tgctcaacat caagcgcctg ctgcaaggca
     6421 tgcaaatctg taccgccttt taccagccag ccgtcaccat cagtaatgat ttccggcgtt
     6481 tcgtcagcgt ccgggaattc acccgcaatg gcttccagca catccagcgg cgtgaccaga
     6541 ccttgtacca caccaaactc gttggtcacg ataacaaagc tcccgcgagc acgacgcagc
     6601 acgcccaaca ggttgatcgg atcgagggtt tccgggacga taatcgccgg agacgccgaa
     6661 gcaatcgccg ccacatcaac gccctcttcc agcgccacca gcagttcttt agcacgtaca
     6721 ataccgatga tttcatccag ttcaccgcga cataccggga acagactgtg cggtgaagag
     6781 agcagttgct cgcggatttc atcgaccccg agattagcgt caacccagct tatttcaccg
     6841 cgcggcgtca tgatcccgcg cagagaacgc gacgccagcg tcagtacgcc gttaatcatg
     6901 taacgttctt cttcggcaaa tgcaccttcc gggatcggca tcggcatcgg gttatcggca
     6961 tcgtgctgaa cattggcctg acgtttcccg cccatcaaac gcaggatggc atcggcagta
     7021 cgcgctcgca gcggcaaagt cgactggtgg cgaataaagt tgcgacgcgc aatctggtta
     7081 aacacttcga tgatgatcga gaagccaatc gcggcataca ggtaaccttt cggaatgtgg
     7141 aaaccgaaac cttctgccac cagactcaga ccaatcatta acaggaagct cagacagagc
     7201 accaccaccg tggggtgctg gttaacgaat cgcgtcagcg gtttggatgc cagcaacata
     7261 accgccatcg caatcactac cgccgccatc atcaccggca gatggttaac catccctact
     7321 gcagtaatta ccgcatccaa cgagaagacg gcgtcaagga tgacaatctg tgtgacgacc
     7381 acccagaaac tggcgtagcc tttaccgtgg ccggaatcat gatcgcggtt ttccagccgt
     7441 tcatgcagtt cggttgttgc tttgaacagc aagaatatcc ccccgaacaa cataatcagg
     7501 tcgcgtccgg agaaggagaa atccatgacg gtaaatagcg gtttggtcag cgtgaccatc
     7561 catgaaatca gcgacagcag ccccagacgc ataatcagcg ccagtgataa ccccagcaaa
     7621 cgcgctttat cgcgttgttt tggcggcagt ttgtcagcaa gaatggcgat gaagaccagg
     7681 ttatcgatac ccagcacaat ttcgagaaca acaagcgtga gtagccccgc ccaaattgag
     7741 gggtccatta agaattccat gacaagctcc tgcttaagga atagctattc gacgccagaa
     7801 ataatgcagg cgtaacgaca aaatgcaaac gaaaggtgcg gcatagagag ccagaaaggt
     7861 aggcgttaaa aggcctgatg ctgaaatgac gtcggtgacg atccatactg cgggctactg
     7921 ccctatactc catggttgtt aaacgggagt taaacatatc agagacgcct ctgatttggc
     7981 aaagatttac cttcctttgc aaacgaatgt gacaaggata ttttaccttt cgaaatttct
     8041 gctaatcgaa agttaaatta cggatcttca tcacataaaa taattttttc gatatctaaa
     8101 ataaatcgcg aaacgcaggg gtttttggtt gtagccctta tctgaatcga ttcgattgtg
     8161 gacgacgatt caaaaataca tctggcacgt tgaggtgtta acgataataa aggaggtagc
     8221 aaatgaccat tgctattgtt ataggcacac atggttgggc tgcagagcag ttgcttaaaa
     8281 cggcagaaat gctgttaggc gagcaggaaa acgtcggctg gatcgatttc gttccaggtg
     8341 aaaatgccga aacgctgatt gaaaagtaca acgctcagtt ggcaaaactc gacaccacta
     8401 aaggcgtgct gtttctcgtt gatacatggg gaggcagccc gttcaatgct gccagccgca
     8461 ttgtcgtcga caaagagcat tatgaagtca ttgcaggcgt taacattcca atgctcgtgg
     8521 aaacgttaat ggcccgtgat gatgacccaa gctttgatga actggtggca ctggcagtag
     8581 aaacaggccg tgaaggcgtg aaagcactga aagccaaacc ggttgaaaaa gccgcgccag
     8641 cacccgctgc cgcagcacca aaagcggctc caactccggc aaaaccaatg gggccaaacg
     8701 actacatggt tattggcctt gcgcgtatcg acgaccgtct gattcacggt caggtcgcca
     8761 cccgctggac caaagaaacc aatgtctccc gtattattgt tgttagtgat gaagtggctg
     8821 cggataccgt tcgtaagaca ctgctcaccc aggttgcacc tccgggcgta acagcacacg
     8881 tagttgatgt tgccaaaatg attcgcgtct acaacaaccc gaaatatgct ggcgaacgcg
     8941 taatgctgtt atttaccaac ccaacagatg tagagcgtct cgttgaaggc ggcgtgaaaa
     9001 tcacctctgt taacgtcggt ggtatggcat tccgtcaggg taaaacccag gtgaataacg
     9061 cggtttcggt tgatgaaaaa gatatcgagg cgttcaagaa actgaatgcg cgcggtattg
     9121 agctggaagt ccgtaaggtt tccaccgatc cgaaactgaa aatgatggat ctgatcagca
     9181 aaatcgataa gtaacgtatt gtgttgatta tcactcagtt ttcacactta agtcttacgt
     9241 aaacaggaga agtacaatgg agattaccac tcttcaaatt gtgctggtat ttatcgtagc
     9301 ctgtatcgca ggtatgggat caatcctcga tgaatttcag tttcaccgtc cgctaatcgc
     9361 gtgtaccctg gtgggtatcg ttcttgggga tatgaaaacc ggtattatta tcggtggtac
     9421 gctggaaatg atcgcgctgg gctggatgaa catcggtgct gcagttgcgc ctgacgccgc
     9481 tctggcttct atcatttcta ccattctggt tatcgcaggt catcagagca ttggtgcagg
     9541 tatcgcactg gcaatccctc tggccgctgc gggccaggta ctgaccatca tcgttcgtac
     9601 tattaccgtt gctttccagc acgctgcgga taaggctgct gataacggca acctgacagc
     9661 gatttcctgg atccacgttt cttctctgtt cctgcaagca atgcgtgtgg caattcctgc
     9721 cgtcatcgtg gcgctgtctg ttggtaccag cgaagtacag aacatgctga atgcgattcc
     9781 ggaagtggtg accaatggtc tgaatatcgc cggtggcatg atcgtggtgg ttggttatgc
     9841 gatggttatc aacatgatgc gtgctggcta cctgatgccg ttcttctacc tcggcttcgt
     9901 aaccgcagca ttcaccaact ttaacctggt tgctctgggt gtgattggta ctgttatggc
     9961 agtgctctac atccaactta gcccgaaata caaccgcgta gccggtgcgc ctgctcaggc
    10021 agctggtaac aacgatctcg ataacgaact ggactaacag gtgagcgaaa tggttgatac
    10081 aactcaaact accaccgaga aaaaactcac tcaaagtgat attcgtggcg tattcctgcg
    10141 ttctaacctc ttccagggtt catggaactt cgaacgtatg caggcactgg gtttctgctt
    10201 ctctatggta ccggcaattc gtcgcctcta ccctgagaac aacgaagctc gtaaacaagc
    10261 tattcgccgt cacctggagt tctttaacac ccagccgttc gtggctgcgc cgattctcgg
    10321 cgtaaccctg gcgctggaag aacagcgtgc taatggcgca gagatcgacg acggtgctat
    10381 caacggtatc aaagtcggtt tgatggggcc gctggctggt gtaggcgacc cgatcttctg
    10441 gggaaccgta cgtccggtat ttgcagcact gggtgccggt atcgcgatga gcggcagcct
    10501 gttaggtccg ctgctgttct tcatcctgtt taacctggtg cgtctggcaa cccgttacta
    10561 cggcgtagcg tatggttact ccaaaggtat cgatatcgtt aaagatatgg gtggtggctt
    10621 cctgcaaaaa ctgacggaag gggcgtctat cctcggcctg tttgtcatgg gagcattggt
    10681 taacaagtgg acacatgtca acatcccgct ggttgtctct cgcattactg accagacggg
    10741 caaagaacac gttactactg tccagactat tctggaccag ttaatgccag gcctggtacc
    10801 actgctgctg acctttgctt gtatgtggct actgcgcaaa aaagttaacc cgctgtggat
    10861 catcgttggc ttcttcgtca tcggtatcgc tggttacgct tgcggcctgc tgggactgta
    10921 agactgttgt acactaccgg ggccttttgg ccccgttttt ttatctggag gattaatgac
    10981 aatcacggac ctggtactga ttcttttcat cgccgcactc ctggccttcg cgatctacga
    11041 tcagttcatc atgccccgcc gtaacggccc caccctgctg gcaattcctt tgctccggcg
    11101 tggtcgcatc gatagcgtta tcttcgtcgg attgattgtc attcttatct ataacaacgt
    11161 cacgaatcat ggtgcgttaa taacgacatg gttattaagc gcactggctc tgatgggttt
    11221 ttatatattc tggatccgcg ttccgaagat catctttaaa caaaaaggtt ttttcttcgc
    11281 caatgtctgg attgaatata gccgaatcaa agcgatgaac ttgtcggaag atggcgtgtt
    11341 ggtgatgcaa ttagaacagc gtcggctgtt aatccgcgtt cgaaatatcg acgatctgga
    11401 aaaaatttat aagcttctcg tttcaactca ataagttatg aatttagcca aagctatgct
    11461 tagtgtattt ttaataatca ga
//
LOCUS       DAZKIV010000146        11463 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZKIV010000146 DAZKIV010000000
VERSION     DAZKIV010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.146"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            173..790
                     /gene="yijF"
                     /locus_tag="ACHOT6_003827"
     CDS             173..790
                     /gene="yijF"
                     /locus_tag="ACHOT6_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFP9948978.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(817..1722)
                     /gene="yijE"
                     /locus_tag="ACHOT6_003828"
     CDS             complement(817..1722)
                     /gene="yijE"
                     /locus_tag="ACHOT6_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFP9948979.1"
                     /translation="MSVAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(1816..3996)
                     /gene="katG"
                     /locus_tag="ACHOT6_003829"
     CDS             complement(1816..3996)
                     /gene="katG"
                     /locus_tag="ACHOT6_003829"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFP9948980.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGSEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYVWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(4325..5215)
                     /gene="metF"
                     /locus_tag="ACHOT6_003830"
     CDS             complement(4325..5215)
                     /gene="metF"
                     /locus_tag="ACHOT6_003830"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFP9948981.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5291..5425
                     /locus_tag="ACHOT6_003831"
     CDS             5291..5425
                     /locus_tag="ACHOT6_003831"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9948982.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            complement(5564..7996)
                     /gene="metL"
                     /locus_tag="ACHOT6_003832"
     CDS             complement(5564..7996)
                     /gene="metL"
                     /locus_tag="ACHOT6_003832"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFP9948983.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(7999..9159)
                     /gene="metB"
                     /locus_tag="ACHOT6_003833"
     CDS             complement(7999..9159)
                     /gene="metB"
                     /locus_tag="ACHOT6_003833"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFP9948984.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVMTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            9436..9753
                     /gene="metJ"
                     /locus_tag="ACHOT6_003834"
     CDS             9436..9753
                     /gene="metJ"
                     /locus_tag="ACHOT6_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFP9948985.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            9937..10545
                     /gene="yiiX"
                     /locus_tag="ACHOT6_003835"
     CDS             9937..10545
                     /gene="yiiX"
                     /locus_tag="ACHOT6_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFP9948986.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYIFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            10690..11365
                     /locus_tag="ACHOT6_003836"
                     /pseudo
     CDS             10690..11365
                     /locus_tag="ACHOT6_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         2798 a         3194 c         2897 g         2574 t
ORIGIN      
        1 tgactttgta ggccggataa ggcgttcacg ccgcatccgg cactctgtgc cagtaatctg
       61 aaactcctac gttgttcagg agttttttat ccgcttctgg acattttctc tacagaacaa
      121 tcgttagccc ggaaggtcga atctgtgact cttattttca cggacgaacc agatgaaagc
      181 ttcactggcg ctgctcagcc tgctaacggc atttaccagc cactccttaa aatctcccgc
      241 cgttccacca accgtggtac aaatccaggc caataccaac cttgccatcg cagacggtgc
      301 cagacagcaa attggcagca cgctatttta cgatccggcg tatatgcagc ttacctatcc
      361 tggcggtgat gtcccgcaag aacgcggggt atgttccgat gtggtgatcc gtgcattgcg
      421 cagccagaag gtcgatttgc agaaactggt gcacgaagat atggcgaaga attttgccga
      481 gtacccgcaa aaatggcagt taaagcgccc ggacagcaac atcgatcacc gtcgggtgcc
      541 taatctggaa acctggttta cccgccacga taaaacacgc cccatcagca agaaccccag
      601 tgactatcaa gcgggcgata ttgtctcctg gcgactggat aacggactgg cacatattgg
      661 ggtggtgtca gatggcttcg cccgcgacgg tacgccgctg gtgatacata acattggcgc
      721 aggcgcgcag gaagaagatg tgctgtttag ctggcggatg gtggggcatt atcgttattt
      781 tgttaaataa gcgatcgccc cacatgaaga aaaaattcag atgcttttta cactgacagc
      841 ttcttttttc ttacggctca ccagcgccag tgccagcaca atcagcacaa taccgctacc
      901 ttcaacggcc cccggattct caccgagcag ccaccaggaa aagagtacgc cgcaaacggg
      961 aacggccagc gtgcttaagc tggcaatact ggcaggcaag tttttcaata caaacaacca
     1021 taagctccac gccagcgccg tcgccagaat cgcactgtag gccagcgccc agaacacggt
     1081 gggctgccag tcaatttcac gttgcggcac cagtaaagcg accacactca tcaccagcgc
     1141 cgcatagagc atctgccagg atgttaacga caataaatcc acgcgcggat gacgggcata
     1201 cagacgttta gcaacaatcg cgctcgcccc ccaactgacg ccggagagga ttgccagcat
     1261 ggcacttttc atcgaagaga aatcgagttg ccacggctgc aacaccaaaa ataagccgaa
     1321 agcggcaatc agtatcgcga aatattgccc acgtcgcagg cgttcaccga gaaacaacgc
     1381 ggcgaaaatc accacccaga acggcatggt atagcttagg atcgccacct tccccgctcc
     1441 gccgctgacc agcgcccact gcgccaggcc aaccatcccg caggtttgta acagggcaat
     1501 ggctaaggtg tatttaaacg gtgtcgggcg cattccgcga ccacgtaata aaaggacgat
     1561 gaataaaacg agagcgccga aaatgcagcg taaggcggta aagtcgaagg caccgatgta
     1621 gctggtgact tgcttcatga aaatccagct ataactccag ataagtgtga gcacaaccag
     1681 gccactgatt gccagtgggt tgctctttcc tgcgacagac atgagagatc cagtgtgtag
     1741 ttttcctgtg gacactatac gccggtaaag aaacttcagc gactgcctga aaggcagtcg
     1801 ctgaagaagg tcagattaca gcaggtcgaa acggtcgagg ttcatcactt tcacccatgc
     1861 cgccacgaag tctttaacaa acttctcgtg agcatcgctg ctggcgtaaa cttcagccac
     1921 cgcacgcagg acagagttag aaccaaatac cagatccgca cggctggcag tgtatttcac
     1981 ttcgccggtt tcgcggtcac ggccttcaaa cagctctttc gattcgtcgg tcgctttcca
     2041 cacgtaacgc atatccagca agttcacgaa gaagtcattg ctcaatacgc caacgcggtc
     2101 agtgaagacg ccgtttttgc tgccatcgaa gttggcaccc agtacgcgca tcccgcccac
     2161 cagcgcggtc atttccggcg cggtcagggt cagttgctgg gctttgtcga tcagcagtga
     2221 ctcggtggtg gaaacatcca gacgagcgcg atagttacgg aaaccgtcag caattggctc
     2281 cagcagctcg aacatctcaa tgtcagtctg atcctgacgc gcatcaacgc ggcccggcgc
     2341 aaacggtaca tgaatgctca aacctgcggc gcttgcggct ttctcaacac caaccacacc
     2401 agccagcacg atgatatccg ccagcgaggc tttaccagac tctttctgga ttttctccag
     2461 aacaggcaga gcacgaacgg ctgcggcgtt cacatcccag tcgcgctgcg gcattaatgc
     2521 cagacgcgca ccgttggcac caccgcgttt gtcgccacca cggaaggtag aagcagaagc
     2581 ccaggccacc gataccagct cactaacaga cagaccagaa tccgcaatcg cgaatttcag
     2641 atcgataatg tcctgctcgg tcgggttgta gatcggctgc ggcagcggat cttgccagat
     2701 cagatcttct ttcggcactt ccgacccgat gtagcgagat ttcggcccca tatccctgtg
     2761 cgtcagtttg aaccaggcac gggcaaaagc ttcgttgaac gcctgcggat cgttgaggaa
     2821 acgacgggag atcttctcga actcaggatc aaaacgcagc gtcaggtcgg tcaccagcat
     2881 tgtcggttta cgtttcttcg acggatcgaa cggatccggg ataatttccg gtgcgtctac
     2941 cgcttcgaac tggattgctc cagccggact gcgggtctgt acccactcat acttgaacag
     3001 attctcgaag aaatagttgc tccactgggt cggcgtctgg gtccagacta cttccagacc
     3061 agaggtaatg gcatctgcgc caacgccgct gccgtaagtg ctcgcccagc ctaaaccttg
     3121 ttcttcaatc ggtgcagctt ctggatcagg acctacgttt gatgtcggac cggcaccgtg
     3181 ggttttaccc agcgtatgac cacccgcaat cagcgccacg gtttcttcgt cgttcatgcc
     3241 catgttgccg aaggtcgcgc ggatagctgc tgccgcagaa agcggttcgc cgctgtgatc
     3301 cgggccttcc gggttaacgt aaatcaggcc catctcggtt gcacccagcg gtgctttcgc
     3361 cagcgcttcc ggatgacggt gagtcagcca ggctttttca tcaccccagt taacatccag
     3421 atccggttcc cagacgtctt cacgaccggc accaaaaccg aaggtacgga agccggagtt
     3481 ttccagcgcc acgttacctg cgaggataaa caggtcggcc caggagattt tctgaccata
     3541 tttctgtttg attggccaca acaggcgacg cgctttatcg aggcttacgt tatccggcca
     3601 ggagttcagc ggtgcaaaac gttgctgacc acgacccgcg ccgccgcgtc catcgattga
     3661 acggtaagta cctgcgccgt gccaggccat acgaataaac agaccggcgt agctgcccca
     3721 gtcggctggc caccacggct gagattctgt cagcagggct ttcagatctt ttttcaggcc
     3781 gtagtaatct aatttgctga attctttgcg gtagtcaaag tcctcaccca gcgggttaga
     3841 acgattagaa tgttggttta acaggtcaac acggagttga tttggccacc agtcgcgagt
     3901 ggttgtgccc gcccccgcgc tctggtcgtg accgccctga tggaacgggc atttgccagt
     3961 ggctgtcgtg ttatggatat cgtctgacgt gctcatcaat gtgctcccct ctacagtgtt
     4021 accgttacga tacacagcgt tagagagaag ttataattga attaatccac ggatgcgata
     4081 gttgagatct tacatattgt tggttaaaga gatgtaggtc aaattgatcg taattaagct
     4141 tttttgatta tgtccgaatt cggacaaatg ctttataaaa aagggtttta gcttcatatc
     4201 cttcaagtaa tgagggagaa aacaaggctg acaaccttat tttcccgccc tcatttcgag
     4261 gcagcatttt gtgctctgtt taaaatttgt gatcactgtg tgattttcac aaaagccaca
     4321 ctatttataa accaggtcga acacccagcg tatggcaaat cgcgtaactc atttcagcgc
     4381 ggttaagcgt atagaagtgg aaatctttca ctccttcacg gcttaaaatc ttcaccatat
     4441 ccatggcaat attcgcgccc accagtttgc gggtttcggc atcatcatcc aggccgtcga
     4501 acatttgcgc catccacgcc ggaatacgca cgttggtcat atcggcaaat ttcttcgcct
     4561 gtttaaagtt agataccggc aaaattcccg gaataatttc cacatcaatg cccgccgata
     4621 cacagcggtc acgaaaacgc aggtagcttt cgacatcgaa gaagaactga gtaatcgcgc
     4681 ggttggctcc ggcatcgact ttgcgtttca gattaagcaa atccgcctga gcgctttttg
     4741 cttccgggtg aacttccgga tacgccgcca cggagatatc gaaatctgcc acttctttta
     4801 atagcgtcac caggtcagaa gcatacattt ctggcttacc acttcccggc ggcagatcgc
     4861 cacgcagcgc cacgatatga cgaataccgt tattccagta gtcgcgtgca atggtgcgca
     4921 actcgtcggg cgtcgcatca atgcaagtaa gatgcggtgc cgcttccaga ccagtgcgat
     4981 ctttaatgcc tttaataatg ctgtgcgtac ggtcgcgctc gccggagttc gcgccatagg
     5041 tcaccgatac aaacttcggt ttcaggctgc taaggcgatc gatggagttc cacagggtct
     5101 gctccatttc actggtacgc ggcgggaaaa actcgaacga aacgttaatc tgcccctgga
     5161 cttctgccag gctctgattc agggcatccc gctggctggc gtgaaaaaag ctcatacctt
     5221 acctcatcaa tcgcttgtag ttatatgttg tgttgtgcac atctatccgt ttagacgtcc
     5281 agatgtaaag atgaaggaaa agccgaaggg cgtcaaccga aaaatgacca atgatggtga
     5341 ggaaaaatca gggaagatga aaaaacttca ggatggtaag aaaaagaaaa tccggtatgc
     5401 aaagcaaacc ggactgcaaa tataaactaa ccatgtactt attgcttctt gccggattcg
     5461 tcgtaaacgc ttaatccggc ctaatcctat gataattcaa tatatttcat tagaaaccat
     5521 aaacccgaaa acatgagtac cgggcattat taaatttctg aaattacaac aactgtgcca
     5581 gccggttgat atccgactga atcgccccgg cggtgacgtc gcgccccgcg ccaggcccac
     5641 ggatcaccag cgggttgtcg cgataccagc ggctttcgat ggcaaagacg ttatcgcacg
     5701 gcagcagtga tgccagcgga tggtcttcac gcaccgcctc cacgcccaca cgcgctttgc
     5761 cgttggcatc gaaacgcgcc acgtagcgca gcaccagccc catttcgcgg gccgcttcca
     5821 gccgttgcac catctgctcg ttaagttcat cgccattttc aaagaaatgg tcgatgctgc
     5881 cgccttcgca atgagcaggc accagcgatt ccacacgtac ctggtccggt tcaatgttgt
     5941 aacccgcttc acgcgccaga attaccagct tgcgcattac gtctttgccg gagaggtcgt
     6001 cacgcggatc aggctcggtt aagccctgct gccacgcctg atccaccagc tcggtaaacg
     6061 gcacgctacc gtcgaattgc aggaacagcc aggagagcgt gccggagaag atcccgctga
     6121 tcgacaaaat agtatcgccg ctgtcgatca gatcgcgcac ggtgtggttg atcggcaagc
     6181 ccgcaccgac ggtggcattg tacagccagt gacgcccggt tttttcgaag gcgtcgtgga
     6241 tctggcgata tttattgctg tcgctggctc ccgccagttt gttggcgctg ataacgtgga
     6301 aaccgtggct ggcgaaatca agatactgat cagcaagctg ctggctggcg gtaacgtcca
     6361 gcaccactaa atcatcatac ggatgggcgc gcatccacag gaacaacgac tcttcatcct
     6421 gctcaaccgc ttcatcgttg aagaaggcta acgcgcggct ggcgtccagc ccgtcatagc
     6481 tcaacaggct gcggcggctg tccaccacac ctgccagcac aaactcaaag ccggtacgtg
     6541 ccgaaagcgt gctctgctca cgggcgaaca gttccagcca acgggaaccg atattgccct
     6601 taccgaacaa taccagaccg atgcgttttt ctgcgcggaa gacggactga tgcagcccct
     6661 gaatcaggct ttcggtcggg ccggtgcgca gtactgccac caggctgatg ccgtcatcgg
     6721 actgccaggt aaattcgacc ggctggcctt tcagttgctg ccagaagcgg tggcaatgca
     6781 gcgggttacg ggtgacgcct gcaccgacca tcgccaccag cgccagcccc tgacgcaggc
     6841 gcagttcgcc aggtaatccc gcttcgtcga ggattttcag cgcactgtcg gccacttctg
     6901 aggtgtagca aaattgcagc aactggcgat cgttatgtac gccaaccgcc agcgggcgta
     6961 cctgcgcgcg tttcaggatt tggtcgatct ctttatgcgc cagtttgaaa tcctgactgg
     7021 cgggcacctg aaactcaatc aaacagacat catcgtggct ggtgacaata cgcgcaccag
     7081 taccggaggc cagcacgcgt tcaatgcgcg tggaaccttg atccggcgtg tagctacagc
     7141 gcagttgcag gtcgatttcg ctgccagaaa ccggctgtaa agtacgggcg tgaagaacgg
     7201 gagccgccag gcgcgccagt tcgctggcct catccagacg cagcaacggc agcaggcagg
     7261 catctttcac tttacgcggg tcggcactgt ataccccggc gacgtcgctc cagatggtta
     7321 cgcgagaaac acccgccagc gcaccgattt gtgtcgcgga atagtcggaa ccgttacgcc
     7381 ccagcagcac cgtttcaccg gcgttgttgc ggctgataaa tccggtcacc accagacgtt
     7441 tgcccggatg ttgcaccagc agctgttgca gcaacgggta agaaagccct tcatcaacct
     7501 gcggttgtgc ggcgcgttca gcgcgtaaaa actcgcgggc atcaagccag gccgctggca
     7561 gcccttgttg attaagtacc gcagacatca ggcgtgccga ccatacttcc ccgtggccca
     7621 ccacttccgc atacactgcg tcgttaatac cgctgtcgag cagcgccgcc aggcgctcaa
     7681 ggtcgctgac aaaagcgcta atgaggctat cggcttcttc agcgggtagc agaccgctaa
     7741 tcagatcgca ctgatagcga cgcagcgttt gttgaacctg atgcgcagag agacgatcgg
     7801 tctggcttag tttcaaccag ttaatcaact ggttagtggt gctaccggcg gcggaaacca
     7861 ccatcatatc gtcaggctga gagtactccg ccataatgcc cgcgacacgc aaataacact
     7921 tcacatcagc cagactactg ccaccaaatt tatgcagctg acgacctttc gcccctgcct
     7981 gcgcaatcac actcattttt accccttgtt tgcagcccgg aagccatttt ccaggtcggc
     8041 aattaaatct tcgccatctt caataccggt ggagatacgc agcagcgtct cggaaatccc
     8101 ggcggcagca cgcgcttctg gtgccatgcc tgcatgtgtc atggttgcgg cgtgagagat
     8161 taaactttcc actcccccta atgattccgc cagcgtaaac aacgacagcc cgcccaggaa
     8221 acgacgcagc gtctgctcat cgccatccag ttcaaaactc aacattgcgc caaagccttt
     8281 ttgctggcgc gcggcaattt catgcccctg attttccggc agcgacgggt gatacagttt
     8341 tttcaccaac ggctgggttt gcagatattt cacaatcgcc tgcgcgttgc gctgggccag
     8401 ctccatacgc ggcaccagcg ttcgcaaccc gcgtagcagc agatagctgt caaacgcgcc
     8461 gcccgtcacg ccaatattgt ttgcccacca ggcaagttca gtgacaacgt ccgggtcttt
     8521 agcaatcacc acgccagcca ccacgtcaga gtgaccgttc agatatttcg tacatgaatg
     8581 caacaccaga tcggcaccca atgccagcgg attttgtaat gccgggctta agaaggtgtt
     8641 atccaccacg ctcaccgccc cgacttccct tgccagatgg cagattttcg caatatccac
     8701 gacgcgtaac aatggattac ttgggctttc taccagtacc agtttgggtt tttctgccag
     8761 tgctgcccgt aatgcctgtt catcgccttg atcaacaaac aacacgcgat agcaaccgcg
     8821 tttcgccagg ctgtcgaaca ggcgatagct accgccgtag cagtcgtgcg gcgcaaccag
     8881 cagatcgcca ggtttcaaaa agacggtcat taccaggtga atcgcggaca tgccggtatt
     8941 agtaagtact gcaccagcac cgccttccag ttctgccagc gcacgctgaa ccacatcgcg
     9001 cgttgggttg ccgcgacgcg agtaatcatg cgcgcgcggt tcattaaatc cggtaaagtt
     9061 atatgtgctg gaaagatgga tcggtgggac aacgcaacca tactgttcgt cgtcatttaa
     9121 cccgctacgc actgcgatgg tggcctgttt acgcgtcatg tgatgaagtt ccctgggctt
     9181 tgtcggtgaa atgtcaggca ccagagtaaa cattgtgtta atggacgtca atacatctgg
     9241 acatctaaac ttctttgcgt atagattgag caaatcccaa atagccgtta aaattatatg
     9301 cattatcacg ccgacaggtg cattacacga tgtcacggta acgcctgtac ggtaaactat
     9361 gcgggtttac ggtcagtacc cacataaatt gtgtggtctg gtctcaattt attgacgaag
     9421 aggattaagt atctcatggc tgaatggagc ggcgaatata tcagcccata cgctgagcac
     9481 ggcaagaaga gtgaacaagt caaaaagatt acggtttcca ttcctcttaa ggtgttaaaa
     9541 atcctcaccg atgaacgcac gcgtcgtcag gtgaacaacc tgcgtcacgc taccaacagc
     9601 gagctgctgt gcgaagcgtt tctgcatgcc tttaccgggc aacctttgcc ggatgatgcc
     9661 gatctgcgta aagagcgcag cgacgaaatc ccggaagcgg caaaagagat catgcgtgag
     9721 atggggatta acccggagac gtgggaatac taaagcggaa atgagcggcg cggagtggaa
     9781 tcgcctgatg cgctacgctt atcaggccta cgtcatattg caatttattg aatttgcacg
     9841 aacttgtagg ccggataagg cgttcacgcc gcatccggca taaacaacga gcacgttgtc
     9901 tgcgacccac cgctttttat acatggacgt ttaactatga aaaacaggct gctgatcctc
     9961 agcctgctgg tttctgtacc tgcctttgcc tggcagccgc aaactggcga catcatcttt
    10021 caaatctctc gctcatcgca aagtaaagcg atccaactgg caacccattc cgattacagc
    10081 cacaccggta tgctggtgat gcgtaacaaa aagccctaca tttttgaagc ggtcggcccg
    10141 gtgaaataca ccccgctcaa gcagtggatt gcccatggtg aaaagggcaa atacgtcgtt
    10201 cgccgcgttg aaggcggact gagtgttgaa caacagcaaa aactggcgca aacggcaaaa
    10261 cgttatctcg gtaaaccgta tgatttcagc ttctcatgga gcgacgatcg ccagtactgt
    10321 tcggaagtgg tgtggaaggt ttaccagaac gcgctgggaa tgcgcgtggg cgaacagcag
    10381 aagttgaaag agtttgatct cagcaacccg ctggtccagg cgaagctcaa agaacgctac
    10441 ggtaaaaata ttccgcttga ggaaacggta gtttcaccac aagccgtttt cgacgcgcca
    10501 caactcacca cggttgccaa agagtggccg ctgttttcgt ggtaatggat gatgaaaaaa
    10561 ataattcccg ccgtagtctg tttgttgctt tgtagttgca cgacctttcc gcaagctgtc
    10621 gctccggtaa acggtgatgc ctccaattag ttgaattaag gatataatac tcgcttttaa
    10681 ggttgtttca tggtcacagt taaccttcat tgcccccgtt gccagtccgt tcaggtttac
    10741 cgtcacgggc agaacccaaa aagacatgat cgattccgcg gccgtgactg tcaccgggta
    10801 tttctgctca gttactgtta cgagccccgt aaaccgggag ttaaagacca aatcgctgaa
    10861 atgactttca acggtgccgg ggttcgcgat actgcccgta cgctgaaaat cggcactaat
    10921 acagtcatcc gaactttaaa aaactctcgc cacgaagaat aacgtcatcc ccggtcgctc
    10981 atgctgatgt ggcactcatc tgcaaactta gtcagcaatg gagctttgtc ggcagtaaag
    11041 cgcgacagca ctggctctgg tacgtgtaca acacgaaaac tggtggtgtt ctggcctaca
    11101 cttttggccc ccggactgac gaaacctgtc gtgaattgcg ggcgctgctt acccttttac
    11161 catcggcatg ttgaccactg acgaatgggg tagttacgct agagagttac cgaaggagaa
    11221 gcatctgact ggtaagattt ttactcagca cattgagcgt aacaatctga ccctgagaac
    11281 ccgaatcaaa cgtctggccc gcagaaccat ctgtttctcg cgttctgtag aacttcatga
    11341 aaaagtcatc ggtgctttta tcgaaaatat atgttctact aattggagtc atcacccttc
    11401 tgataagctt tccttggatg attaccagac tgaatacaca gccaaagatt tttacctcat
    11461 tct
//
LOCUS       DAZKIV010000147        11441 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZKIV010000147 DAZKIV010000000
VERSION     DAZKIV010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.147"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..971
                     /locus_tag="ACHOT6_003837"
     CDS             <1..971
                     /locus_tag="ACHOT6_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFP9948987.1"
                     /translation="FLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSS
                     DDKDVIAIDGKTLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIETDEKSNEITAIPEL
                     LNMLYIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELN
                     NPEHDSYAISEKSHGREEIRLHIVCDVLDELIDFTFEWKGLKKLCVAVSFRSIIAEQK
                     KEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAEL
                     FSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            1240..3477
                     /gene="nrfB"
                     /locus_tag="ACHOT6_003838"
     CDS             1240..3477
                     /gene="nrfB"
                     /locus_tag="ACHOT6_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFP9948988.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            3464..6436
                     /gene="nfrA"
                     /locus_tag="ACHOT6_003839"
     CDS             3464..6436
                     /gene="nfrA"
                     /locus_tag="ACHOT6_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFP9948989.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLIEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            6437..7327
                     /locus_tag="ACHOT6_003840"
     CDS             6437..7327
                     /locus_tag="ACHOT6_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFP9948990.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7510..8271
                     /gene="envY"
                     /locus_tag="ACHOT6_003841"
     CDS             7510..8271
                     /gene="envY"
                     /locus_tag="ACHOT6_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFP9948991.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLIPHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8784..9737
                     /gene="ompT"
                     /locus_tag="ACHOT6_003842"
     CDS             8784..9737
                     /gene="ompT"
                     /locus_tag="ACHOT6_003842"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFP9948992.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9924..11408
                     /locus_tag="ACHOT6_003843"
     CDS             9924..11408
                     /locus_tag="ACHOT6_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFP9948993.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         3115 a         2789 c         2768 g         2769 t
ORIGIN      
        1 atttcttgaa gcaatatggt gattttgaaa atggtattcc tgttcacgat actattgcca
       61 gagttgtatc ctgtatcagt cctgcaaaat ttcatgagtg ctttattaac tggatgcgtg
      121 actgccattc ttcagatgat aaagacgtca ttgcaattga tggaaaaacg ctccggcact
      181 cttatgacaa gagtcgccgc aggggagcga ttcatgtcat tagtgcgttc tcaacaatgc
      241 acagtctggt catcggacag atcgagacgg atgagaaatc taatgagatt acagctatcc
      301 cagaacttct taacatgctg tatattaaag gaaaaatcat cacaactgat gcgatgggtt
      361 gccagaaaga tattgcagag aagatacaaa aacagggagg tgattattta ttcgctgtaa
      421 aaggaaacca ggggcggcta aataaagcct ttgaggaaaa atttccgctg aaagaattaa
      481 ataatccaga gcatgacagt tacgcaatta gtgaaaagag tcacggcaga gaagaaatcc
      541 gtcttcatat tgtttgcgat gtccttgatg aacttattga tttcacgttt gaatggaaag
      601 ggctgaagaa attatgcgtg gcagtctcct ttcggtccat aatagcagaa caaaagaagg
      661 agccagaaat gacggtcaga tattatatca gttctgctga tttaaccgca gagaagttcg
      721 ccacagcaat ccgaaaccac tggcacgtgg agaataagct gcactggcgt ctggacgtgg
      781 taatgaatga agacgactgc aaaataagaa gaggaaatgc agcagaatta ttttcaggga
      841 tacggcacat cgctattaat attttaacga atgataaggt attcaaggca gggttaagac
      901 gtaagatgcg aaaagcagcc atggacagaa actatctcgc gtcagtcctt gcggggagcg
      961 ggctttcgta atcttgccct gcctgctcac ctcctccagg cacttccact acatgatgac
     1021 ctgctcggtg ccaccgctcc ttttcactat gacgttaacc tcccctcctt gacaccatgc
     1081 ttttgtctac attctacgca aacggcaact tttataacca gtgtaaaaat aacgtgccgc
     1141 aatagtcata cctattaatg gtaaaaagct gtcacaattc ataaaaaacc ttaatatacg
     1201 ccaccctaaa cataaccagc gttaatgtaa ggtttttgtg tggactggct tcttgatgtt
     1261 tttgcaacct ggctctacgg cttaaaagta atcgcgataa cgttagcggt catcatgttc
     1321 atcagcgggc tggacgattt ttttattgat gtcgtctact gggtacgccg cattaaacgc
     1381 aagttgagtg tttatcgccg ctacccgcga atgagttacc gcgaactgta taaaccagat
     1441 gaaaaaccgt tagcgattat ggttccggcg tggaatgaaa cgggcgtcat cggcaatatg
     1501 gccgagctgg cggcgaccac gctcgactac gaaaactatc atatctttgt tggcacctac
     1561 cccaacgacc ctgatactca gcgtgatgtt gacgaagtgt gcgctcgctt cccgaatgtg
     1621 cataaggtag tctgcgcgcg tcctggcccc accagcaaag ccgactgtct gaacaacgtg
     1681 ctggacgcca tcacccagtt tgaacgtagc gccaatttcg cttttgctgg ttttattctg
     1741 catgacgccg aagatgtgat ttcaccgatg gaattgcgtc tgttcaacta cctggtcgag
     1801 cgtaaagatc tgattcagat cccggtgtat ccgtttgaac gcgaatggac gcacttcacc
     1861 agcatgactt acattgatga gttttcagag ctgcatggca aagatgttcc ggtgcgtgaa
     1921 gccctcgccg gacaagtgcc cagcgcaggc gtcggcacct gtttcagccg ccgcgccgtg
     1981 accgctctgt tagctgacgg tgacggtatt gctttcgacg tgcagagtct gactgaagac
     2041 tacgacattg gcttccgcct gaaagaaaaa ggtatgacgg aaatttttgt ccgttttccg
     2101 gtggtggacg aagccaaaga acgcgagcag cgtaaatttt tacagcacgc acggacgtca
     2161 aacatgatct gcgtgcgcga atatttcccg gatacctttt cgactgcggt tcgacaaaaa
     2221 tcccgctgga tcatcggcat tgttttccag ggctttaaaa cccataaatg gacctccagc
     2281 ctgacgctga actactttct ctggcgcgac cgcaaagggg caatcagtaa ctttgtcagc
     2341 ttcctcgcga tgctggtgat gatccagctt ttgctgttgc tggcgtatga aagtttgtgg
     2401 cccgatgcct ggcatttcct ttctattttc agcggcagcg catggttaat gaccctgctg
     2461 tggctaaact ttggtttgat ggttaaccgc atcgtgcagc gggtgatttt cgttactggc
     2521 tactacggcc tgacgcaggg cctgctttcc gtcctgcgtc ttttctgggg caacctgatt
     2581 aactttatgg ctaactggcg cgcgctgaaa caggttcttc agcacggcga tccgcgtcgc
     2641 gtggcgtggg ataaaacaac gcatgacttc cccagcgtca ctggcgatac ccgctcgttg
     2701 cgcccgttag gtcaaattct gctggaaaat caggtcatca ctgaagaaca actcgataca
     2761 gcactgcgta atcgcgtcga aggtctacgc ctgggcggtt caatgctgat gcaggggctg
     2821 attagcgccg agcagctggc acaggcgctg gcagagcaaa acggcgtggc gtgggaatcc
     2881 atcgatgcct ggcagatccc gtcctcgctg attgccgaaa tgccggcctc cgtggcgctg
     2941 cattatgcgg tactgccgct gcgtctggaa aatgatgagt taattgtcgg cagtgaagat
     3001 ggtattgacc cggtttcgct ggcggccctg acgcgtaaag tcggacgcaa agtgcgttac
     3061 gtcattgttc tgcggggaca aattgtcaca gggttacgtc actggtatgc acgccgacgc
     3121 ggtcacgatc cgcgggcaat gttgtacaat gcggttcagc atcagtggct cacggaacag
     3181 cagaccggtg aaatctggcg gcaatatgtg ccgcatcagt tcctgttcgc cgaaatactg
     3241 accacgctcg gtcatattaa tcgttcagca attaacgtgt tgttattgcg ccatgaacgc
     3301 agttctctgc cgctcggcaa gtttttggtc accgaaggcg ttatcagcca ggaaacgttg
     3361 gatcgcgtcc tgacaattca acgcgaatta caagtttcga tgcaatcact attactcaaa
     3421 gcaggtttaa acacagaaca ggttgcgcaa ctggagtccg aaaatgaagg agaataacct
     3481 taatcgcgtc atcggatggt ctggtttact gttgacgtct ttattgagta ccagcgcact
     3541 cgcagacaat attggcacca gcgcagaaga gctggggctg agcgattatc gccattttgt
     3601 tatttatccc cgtctcgata aggcgctgaa ggcacagaaa aataacgacg aagcaaccgc
     3661 catccgcgaa tttgaatata tacaccagca ggtgccggat aatattccgc tgactttata
     3721 ccttgcggaa gcctatcgcc attttggtca tgatgaccgg gcgcggctgt tgcttgagga
     3781 tcaactgaaa cgtcacccag gagatgcccg acttgagcgc agtctggcgg ctattccggt
     3841 tgaagtgaaa agcgttacga cagttgaaga actgcttgcc cagcaaaaag cgtgcgatgc
     3901 tgcgccgacc ctgcgttgtc gcagtgaagt cgggcagaat gccctgcggc tggcacagtt
     3961 acctgtcgcc agagcgcaac tgaacgatgc gacgtttgct gcatcgccgg aaggaaaaac
     4021 gctgcgaacc gatctgctgc aacgggcaat ctacctgaaa caatggtccc aggcagatac
     4081 gctatacaat gaagcacgcc agcagaacac attaagcgcg gcagaacgcc gtcagtggtt
     4141 tgacgtgctt cttgccgggc agctggacga tcggatcctg gcactgcaat cacaggggat
     4201 cttcaccgat cctcagtcat atattactta cgcgaccgcg ctggcttatc gtggcgaaaa
     4261 agcacgcctc cagcattatc tcattgaaaa taagccgctg tttaccacgg acgcacaaga
     4321 gaaaagttgg ctctatctgt tatctaaata cagcgccaac cccgttcagg cattggcgaa
     4381 ttatacggtg cagtttgccg ataaccgcca gtatgttgtt ggcgcgacgc taccggtgct
     4441 gttaaaagaa ggtcagtacg acgcagcgca aaaactgctc gccaccctcc ccgccaatga
     4501 aatgcttgag gagcgttatg ctgtcagcgt ggcgacccgt aacaaggctg aatctctgcg
     4561 tctggcacga ttgctgtatc agcaagaacc ggcaaatctt acccgcctgg atcaactgac
     4621 ctggcaactg atgcagaacg agcagtcacg cgaagctgcc gatttattgc tgcaacgcta
     4681 tcctttccag ggcgatgcgc gtgtcagcca gactttaatg gcgcgactgg cgtctctgct
     4741 ggaaagtcat ccttacctgg caacgccggc gaaggtggcg attttatcga aacccttacc
     4801 gctggcggag caacgtcagt ggcaaagtca gttgccgggt attgcagata attgcccggc
     4861 aatagttcgc ttgctgggcg atatgtcgcc ttcctacgat gctgccgcct ggaaccgtct
     4921 ggcaaagtgt tatcgggaca cgctacccgg tgtggcgctg tatgcatggc ttcaggccga
     4981 acaacgacaa ccgaacgcct ggcaacatcg tgcggtagcc tatcaggcgt atcaggttga
     5041 ggactacgcc accgcactgg cggcctggca gaaaatcagt cttcacgaca tgagcaatga
     5101 ggatctgctt gctgctgcca ataccgccca ggcggcagga aatggtgcag ctcgcgatcg
     5161 ctggctacaa caggcagaac aacgtggact gggaaacaat gccctctact ggtggctgca
     5221 tgcgcaacgt tacattcctg gtcagccgga actcgcactg agcgatctca cgcgctcaat
     5281 caatattgcg ccttctgcca acgcttacgt tgcgcgggcg acaatttatc gccaacgtca
     5341 taatgtcccg gcggcggtga gtgatttgcg cgccgcgctg gaactggaac cgaataatag
     5401 caacatccag gcagcgcttg gttacgcctt gtgggatagc ggtgatatcg cacagtcgcg
     5461 ggaaatgctc gaacaggcgc ataaagggct accggacgat ccggcactga tccgacaact
     5521 ggcctatgtg aaccagcgtc tggatgacat gcctgcgacg cagcactacg cccggctggt
     5581 gattgatgac attgataatc aggcgctgat aaccccactg accccagagc aaaatcagca
     5641 acgcttcaat ttccgccgtt tgcatgagga ggtcggtcgc cgctggacgt tcagtttcga
     5701 ttcttccatc ggcttgcgtt ccggcgcaat gagtaccgct aacaataatg tcggcggcgc
     5761 agcgccaggg aaaagctatc gtagctacgg acaactggaa gccgagtacc gcatcggacg
     5821 caatatgctg ctggaaggcg acctgctctc agtttatagc cgcgtctttg ccgataccgg
     5881 agaaaacggg gtgatgatgc cggtgaaaaa tccgatgtcc ggcaccggtc tgcgctggaa
     5941 gccgctgcgc gatcagatct ttttcctcgc cgtcgaacag cagttgccgc tgaacggcca
     6001 aaatggcgca tccgatacca tgctgcgcgc cagcgcctca ttctttaatg gcggcaaata
     6061 cagcgacgaa tggcacccga acggttcagg ctggtttgcc caaaacctgt acctcgatgc
     6121 ggcgcaatat atccgccagg atattcaggc gtggacggca gattatcgcg tcagctggca
     6181 tcagaaggta gctaacggac agactattga gccttacgct cacgttcagg acaacggcta
     6241 tcgtgataaa ggcactcagg gcgcgcagct tggcggagtc ggggtccgct ggaatatctg
     6301 gaccggcgag acgcactacg acgcctggcc gcacaaagtc agtctcggcg tcgagtatca
     6361 acataccttt aaggcgatta atcaacgtaa cggagagcgc aacaacgcgt ttctcaccat
     6421 tggagtgcac tggtaaatgc gtaagttcat tttcgtattg ctgacactgc ttttggtcag
     6481 ccctttttcc tttgcgatga aaggtattat ctggcaacca caaaaccgag atagtcaggt
     6541 taccgatacc cagtggcagg ggctgatgag tcagttacgt ttgcaaggct tcgataccct
     6601 ggttttgcaa tggacccgtt acggcgatgc attcacccag ccagaacagc gcgcgttatt
     6661 gtttaagcgt gccgcagctg cgcaacaggc tggcctgaag ctgattgtcg ggctgaacgc
     6721 cgatccggaa ttttttatgc accagaaaca gtcgtccgca gcgctggaaa gctatcttaa
     6781 tcgcctgctg gctgccgatc tccagcaagc cagattatgg agcgccgcgc ctggcgtaac
     6841 gccggatggc tggtacatca gcgcggaaat tgacgacctg aactggcgca gcgaagctgc
     6901 ccgtcagcct ttgctaacat ggttaaacaa cgcgcagcgg ctgattagcg atgtttcagc
     6961 aaaaccggtt tatatcagta gttttttcgc cggaaacatg tcgcccgatg gctatcgcca
     7021 actgctggaa caagttaaag caaccggcgt taatgtctgg gtacaggatg gcagcggcgt
     7081 ggataaactt accgctgaac agcgtgaacg ttatctacag gccagcgccg attgccaaag
     7141 tcccgcccct gccagcggca ttgtttatga actgtttgtc gccggcaaag gcaaaacctt
     7201 tacagcgaaa ccgaaaccgg atgcagagat cgcctcgctg ttagcgaaac gttcctcttg
     7261 cggtaaagac actctctatt tctctctgcg ctatttgccc gtcgcgcacg gcattctcga
     7321 gtattaaatc tcctccaggt aagtcgggta cgacctggct tacctctttc gctctcgtca
     7381 gaattccatc aagattaatt gctaaaaagc gtgctaaaga aagaagatcg ttatcggcga
     7441 acgggccgat cactcatgca ggaagcatag atcctttgag cggagtttct gatatctgag
     7501 gagtgcgaaa tgcaattgag cagcagtgaa ccttgcgtgg tgatcctgac cgaaaaagag
     7561 gtagaggtaa gcgtcaataa ccatgctacg tttacccttc cgaaaaacta cctggccgcc
     7621 ttcgcgtgca acaataacgt cattgaactc tcaacgttaa atcacgtatt aatcccccac
     7681 atcaaccgta acatcatcaa cgattatctg ttgtttttaa ataagaactt aacctgtgta
     7741 aagccctggt cgcggctggc aaccccggtt atcgcttgtc atagccgtac accggaagtg
     7801 ttccggctag ccgccaacca cagcaagcag caacccagca aaccctgcga agcggagttg
     7861 acgcgcgcat tactttttac cgtattgtct aactttcttg agcaatcgcg gtttatagcc
     7921 ctactgatgt atatcttacg cagcagcgtc cgcgacagcg tctgccgcat tattcaaagc
     7981 gatattcagc attactggaa tctgcgaatt gtcgccagtt cgctatgttt aagccccagc
     8041 ctactcaaaa agaaattaaa aaacgaaaat accagctata gccagattgt cacagagtgt
     8101 cgtatgcgtt acgccgtaca gatgttattg atggataaca aaaatatcac tcaggtggcg
     8161 caattatgtg gctatagcag cacgtcgtac tttatctctg tttttaaggc gttttacggc
     8221 ctgacaccgt tgaattatct cgccaaacag cgacaaaaag tgatgtggtg aagggcaaag
     8281 cggaaacgga taagacgggc ataaatgaag aagaaatggc gcgccctgca ggattcgaac
     8341 ctgcggccca cgacttagaa gttcctagaa cgacatttta agtcaacaaa ttaccgcgcc
     8401 atctctgcgc tcacacgtcc cactacctca aaacatgtaa agccttgcaa gccattgtga
     8461 ggccttatgt gtctcagttt tgtccctctt ttttgtacta aaaaacatag caattgagga
     8521 taaacctcat gctattttcg cttatatgcc tttaaaggca tggcgcttaa atagataaaa
     8581 acaccacaaa agcataaaaa accacacagt aaaaccgcaa cataaaacaa taacagataa
     8641 ttaaaccaaa aacagatagc gcattgtgat aatcattcaa tactaaacaa aatataaaca
     8701 gtggagcaat atgtgattga ctcattaagt tagatataaa aaatacatat tcaatcatta
     8761 aaacgattga atggagaact tttatgcggg cgaaacttct gggaatagtt ctgacaaccc
     8821 ctattgcgat cagctctttt gcttctaccg agactatatc gtttactcct gacaacataa
     8881 atgcggacat tagtcttgga actctgagcg gaaaaacaaa agagcgcgtt tatctagctg
     8941 aagaaggagg ccgaaaagtc agtcaactcg actggaaatt caataacgct gcaattatta
     9001 aaggtgcaat taattgggat ttgatgcccc agatatctat tggggctgcg ggctggacta
     9061 ctctcggcag ccgaggtggc aatatggtcg atcaggactg gatggattcc agtaaccccg
     9121 gaacctggac agatgaaagt aggcaccctg atacacaact caattatgcc aacgaatttg
     9181 atctgaatat caaaggctgg ctcctcaacg aacccaatta ccgcctggga ctcatggccg
     9241 gatatcagga aagccgttat agctttacag ccagaggtgg ttcctatatc tacagttctg
     9301 aggagggatt cagagatgat atcggctcct tcccgaatgg agaaagagca atcggctaca
     9361 aacaacgttt taaaatgccc tacattggct tgactggaag ttatcgttat gaagattttg
     9421 agctaggtgg cacatttaaa tacagcggct gggtggaagc atctgataac gatgagcact
     9481 atgacccagg aaaaagaatc acttatcgca gtaaagtcaa agaccaaaat tactattctg
     9541 ttgcagtcaa tgcaggttat tacgtaacac ctaacgcaaa ggtttatgtt gaaggcgcat
     9601 ggaatcgggt tacgaataaa aaaggtaata cttcacttta tgatcacaat gataacactt
     9661 cagactacag caaaaatggt gcaggcatag aaaactataa cttcatcact actgctggtc
     9721 ttaagtacac cttttaacga agttaaccag attttctccc cggtggtatt gtataacccc
     9781 ggggaatttg tttacagtta caagtaggca atatccacta attttgtaag catttaacct
     9841 atgtcataac gtgtaatctc ttaccgcaac acaagacctc aatatattca aatcttatct
     9901 ttattccgat attcagagac acaatgcgaa aaataattac tcatttcaaa gttgttttaa
     9961 cgttacttct accagtaacc gtatctgccc agcagataca gtggcaatcc tgtatggcca
    10021 gtcaattcaa ccactggttt ggtgaggaaa aaccgtctcc tgacttacta tgtggttatt
    10081 tgtctgttcc attaaaatat acagacacag gcggagatgc ttcttatgaa aaaaaatcac
    10141 aagtcaaact agcgttgaca aaattgccgg caaaaagcaa gcataaagga agtatcctga
    10201 taataagtgg tggtcccggg ttaccaggca taaatcctta tattaacttt gactggccag
    10261 tcacaaatct tcgtgagtca tgggatatta ttggatttga tcctcgaggc gtcggacagt
    10321 ccactccgac aataaactgc cggcaatcag atacagagac tcaggaaaac ataaccgaaa
    10381 agcaacaagt attaaataaa attaatgcct gtatccataa taccggagcc gaagtcattc
    10441 gccatatcgg ctctaacgag gctgtatacg atattgatcg tattaggcaa gccttggggg
    10501 ataaacaact gacagccgtg gcgtattcgt atggaactca aattgcagcc ttatatgcag
    10561 aacgttttcc ctacaacgta agatctatcg ttcttgatgg agtcgtcgat atcgatgacc
    10621 tggaggacaa cttcacatgg caactcaaac aggcacagag ttatcaggaa acgtttgatc
    10681 gctttgcatc ctggtgtgcg cgtacaaaaa gttgcccgct ttcttcagac agagataagg
    10741 caataactca gttccatgag ctattatcaa aattacatca caaaccttta ttagacagta
    10801 agggagaaaa tatatcttca gatgaactca tatcattaac aacagacctt ctgctatggc
    10861 gttcatcatg gccaaccctt gcaactgcca tacgccagtt ctctcagggg attgtcagta
    10921 atgaaattga aactgcgctc agtgctccga tagcctcaga agagtcaagc gatgcttcgg
    10981 gggtaatcct ctgtgtagat cagggggatg agcaattaac accagaagag cgaaaatccc
    11041 gaaaagacgc tcttgcgaat gccttcccgg ctattaactt tgacaatgga cgttccgatt
    11101 cacctgattt ttgtgaatta tggccaatac atagcgacct gaacaaaact cgcctgaaaa
    11161 atactgttct gccctctggt ttactgtttg tagcacacaa atacgaccca acaacgccct
    11221 ggattaatgc ccgtaagatg gcagagaaat tttccagccc gttactaaca ataaatggtg
    11281 atgggcatac attagctctc accggagtta atttatgtgt agataaagca gttgtacatc
    11341 acctgatcac tccacaaaaa atagaaaata tatactgccc aggaaattct gaagcagaaa
    11401 tacaataatt tcaaaatatt ccgctatttg ctcccaatgc a
//
LOCUS       DAZKIV010000148        11360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZKIV010000148 DAZKIV010000000
VERSION     DAZKIV010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.148"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            185..727
                     /gene="ecpR"
                     /locus_tag="ACHOT6_003844"
                     /gene_synonym="matA"
     CDS             185..727
                     /gene="ecpR"
                     /locus_tag="ACHOT6_003844"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFP9948994.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            802..1389
                     /gene="ecpA"
                     /locus_tag="ACHOT6_003845"
                     /gene_synonym="matB"
     CDS             802..1389
                     /gene="ecpA"
                     /locus_tag="ACHOT6_003845"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFP9948995.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1447..2115
                     /gene="ecpB"
                     /locus_tag="ACHOT6_003846"
                     /gene_synonym="yagY"
     CDS             1447..2115
                     /gene="ecpB"
                     /locus_tag="ACHOT6_003846"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFP9948996.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2141..4666
                     /gene="ecpC"
                     /locus_tag="ACHOT6_003847"
                     /gene_synonym="yagX"
     CDS             2141..4666
                     /gene="ecpC"
                     /locus_tag="ACHOT6_003847"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFP9948997.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRTQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4656..6299
                     /gene="ecpD"
                     /locus_tag="ACHOT6_003848"
     CDS             4656..6299
                     /gene="ecpD"
                     /locus_tag="ACHOT6_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFP9948998.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIASLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6268..6978
                     /gene="ecpE"
                     /locus_tag="ACHOT6_003849"
     CDS             6268..6978
                     /gene="ecpE"
                     /locus_tag="ACHOT6_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFP9948999.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGEQLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7291..7620
                     /gene="ykgJ"
                     /locus_tag="ACHOT6_003850"
     CDS             7291..7620
                     /gene="ykgJ"
                     /locus_tag="ACHOT6_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFP9949000.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7868..8482)
                     /gene="yagU"
                     /locus_tag="ACHOT6_003851"
     CDS             complement(7868..8482)
                     /gene="yagU"
                     /locus_tag="ACHOT6_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HFP9949001.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8900..9589
                     /gene="paoA"
                     /locus_tag="ACHOT6_003852"
     CDS             8900..9589
                     /gene="paoA"
                     /locus_tag="ACHOT6_003852"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFP9949002.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9586..10542
                     /gene="paoB"
                     /locus_tag="ACHOT6_003853"
     CDS             9586..10542
                     /gene="paoB"
                     /locus_tag="ACHOT6_003853"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFP9949003.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPLGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10539..>11360
                     /gene="paoC"
                     /locus_tag="ACHOT6_003854"
     CDS             10539..>11360
                     /gene="paoC"
                     /locus_tag="ACHOT6_003854"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP9949004.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPT"
BASE COUNT         2924 a         2983 c         2935 g         2518 t
ORIGIN      
        1 cataaattta agcccaatcg aaaataatta aacttaatct cgtttaacct ttattgatat
       61 gtactacgta tcttatttac ttccggttta ctaaggaaac tgaatgcacc tgtaaaaatt
      121 acaggtttgg aaagtagtga catggcaaag tgattacagt agggactatg aggttaaaaa
      181 ccatatggaa tgtcaaaacc gttctgataa atacatctgg tctccccatg acgcctactt
      241 ctataaagga ctatctgaac tgattgtgga tatcgacaga ttaatttatc tatcgttgga
      301 gaaaattaga aaagatttcg tgtttatcaa tctcaatacg gattctttat ctgaatttat
      361 aaaccgtgat aatgaatggt tatccgcggt aaaggggaaa caggtcgtat tgattgcggc
      421 cagaaagtca gaagccttag caaattattg gtattacaac agcaatatta ggggcgtggt
      481 atacgcagga ctgagtcgtg atattagaaa agaactggcc tatgtgatta atggcaggtt
      541 cctgagaaaa gatattaaga aagataaaat cacggaccgg gaaatggaaa ttatccgcat
      601 gacggcccag ggaatgcaac ctaaatcgat tgccagaatt gaaaattgta gtgtgaagac
      661 agtgtatacc catcggcgta atgctgaggc caagctgtac tcaaaaatat ataagttggt
      721 tcagtaaact ccaggcaagt tagttttaaa aaatgactca ctgggacatc acgtcctcaa
      781 ttcaactcgg gaagaaatgc aatgaaaaaa aaggttctgg caatagctct ggtaacggtg
      841 tttaccggca tgggtgtggc gcaggctgct gacgtaacag ctcaggctgt agcgacctgg
      901 tcggcaacag ccaaaaaaga caccaccagt aagctggttg tgacgccact cggtagcctg
      961 gcgttccagt atgccgaagg cattaaaggt tttaactcac agaaaggtct atttgacgtg
     1021 gctatcgagg gtgactcaac ggctaccgcc tttaaactga cctcacgtct tatcaccaac
     1081 acattaaccc agttggatac ctcaggttcc acactgaatg tgggcgtgga ttataacggc
     1141 gcggcagtcg aaaaaactgg cgataccgtg atgatcgata ccgccaacgg cgtactgggc
     1201 ggcaacctta gcccgctggc taacggttac aatgccagca atcgtaccac cgcacaggat
     1261 ggtttcacct tctccatcat cagcggtacc accaatggta ccaccgcagt aacagattac
     1321 agcactctac cggaaggcat ctggagcggc gacgttagcg tacagttcga cgcgacctgg
     1381 accagttaat ctctctgatg taccagcagg ggtagccccc ctgctttatt cctggacgga
     1441 attgttatga aaaagcacct tctgcctctc gctctgctgt tttccggaat atctccggcc
     1501 caggcgctgg atgtcggcga tatatcatcg tttatgaaca gtgacagcag cacgctgagc
     1561 aaaacgatca aaaacagtac cgacagtggt cgccttatca atatccgtct cgaacggctc
     1621 tcttcaccgc ttgacgacgg gcaggttatc tcaatggaca agccggatga gttgctactc
     1681 actcccgcca gcttgctgct acccgcccaa gccagcgaag tgatccgctt cttctataag
     1741 ggacccgcag atgaaaaaga gcgctactac cgcattgtct ggtttgatca ggccctcagt
     1801 gatgcgcagc gcgataatgc caaccgcagc gctgtggcca ctgcttccgc ccgcatcggc
     1861 accattctgg tcgtcgcccc tcgtcaggcg aactaccact ttcagtacgc caacggctcc
     1921 ctgacaaata caggaaatgc gacgctgcgg atcctcgctt acggcccctg cctgaaagcc
     1981 gccaatggta aggagtgtaa agagaattac tacctgatgc caggcaagtc gcgtcgtttt
     2041 acccgcgtgg acacggcgga taacaaagga cgggttgcac tttggcaggg tgataagttc
     2101 attcccgtga aatagatagc tgtgcagatg gataacgaca atgcctttac gacggttctc
     2161 cccaggactg aaagcccagt ttgccttcgg catggtcttt ttgttcgttc aacccgatgc
     2221 cagcgctgct gacataagtg cgcagcaaat aggtggggtg attattccgc aggccttcag
     2281 tcaggcgctt caggacggca tgagcgtccc gctctatatt catctcgccg gtagccaggg
     2341 tcgccaggac gatcagcgaa tcggcagcgc ttttatctgg ctggatgatg gacagctacg
     2401 catccggaaa atacagctgg aagagagtga agataacgcc agtgtcagcg aacaaactcg
     2461 acagcagctg atggctctgg cgaacgcccc gttcaatgag gcccttacca tccccctgac
     2521 tgacaacgcg cagctggatc tcagcttgcg ccaactgctg ctgcagctgg tggtcaagcg
     2581 cgaagcgctg ggcaccgtac tacgctcacg tagcgaagac atcgggcagt ccagtgttaa
     2641 caccctcagc agtaatctga gctataactt gggcgtctat aacaaccagt tgcgtaacgg
     2701 cgggagcaac acatccagct atctgtcgct gaataacgtt actgcgctgc gcgaacatca
     2761 tgtggtgctc gacggctcgc tgtacgggat cggtagcggt caacaggaca gtgaattata
     2821 taaagcgatg tatgaacgcg attttgccgg tcaccgattt gccggtggaa tgctcgacac
     2881 ctggaacttg cagtccttag ggccgatgac cgccatttca gcagggaaga tttacggcct
     2941 ttcctgggga aaccaggcca gctccaccat cttcgacagc agccagtcag ccacgccagt
     3001 gatcgccttt ttaccggcgg cgggcgaagt acatctcacc cgtgatgggc ggctactaag
     3061 cgttcagaac ttcactatgg gcaatcatga agtggatacc cggggtctac cgtacgggat
     3121 ttacgatgtg gaagttgagg tgatcgttaa cggtcgcgtg atcagcaaac gcacccagcg
     3181 ggtcaataag ctgtttagcc gggggcgcgg cgtcggtgca ccactggcgt ggcaggtatg
     3241 gggcggtagc tttcatatgg atcgctggtc ggaaaacggg aaaaagacgc gaccagctaa
     3301 agagagttgg ctagcaggtg cctcgacctc cggctcattg agtacgctta gctgggcggc
     3361 aacgggatat ggatacgata atcaggcggt gggtgaaacc cgtctgacgc tgccgcttgg
     3421 gggggcgatc aacgttaacc tgcaaaatat gctggccagt gacagctcat ggagcagcat
     3481 cggcagcatc agcgccactc taccgggagg ctttagttcg ctgtgggtta accaggaaaa
     3541 aacccgcatt ggcaatcaat tgcgacgtag cgatgccgac aaccgtgcaa tcggcggcac
     3601 actcaacctg aactcactgt ggtcgaagct gggcacattc agcatcagct acaatgatga
     3661 ccgccgttac aacagccatt attacacggc agattactat caaaatgtct acagcggtac
     3721 ctttggttcg cttggcctgc gggccggtat tcagcgctat aacaacggcg acagcaacgc
     3781 caatacaggg aaatatatcg ctctcgatct ctcgctacca ctgggcaact ggtttagcgc
     3841 agggatgact catcaaaacg gctacaccat ggcaaacctg tcagcacgca aacagtttga
     3901 tgaaggaacc attcgcactg ttggtgccaa tctgtcacgt gccatctccg gcgataccgg
     3961 tgatgacaaa accctcagcg gtggggcgta tgcacaattc gacgctcgtt acgccagcgg
     4021 aacgctgaac gtcaatagcg cggcggacgg ctacgtcaat accaacttga ccgccaatgg
     4081 cagcgtcggc tggcagggta aaaacattgc tgccagcggg cggactgatg gcaacgctgg
     4141 ggtgatattc aacaccgggc tggaggacga cggtcagatc agcgccaaaa tcaacgggcg
     4201 gattttcccg cttaacggca agcgtaacta tctcccgctc tctccctatg gaagatatga
     4261 ggtggagtta cagaacagca aaaactcact cgacagctac gatatcgtca gcggccgcaa
     4321 aagccatctg actctctatc caggcaatgt cgctgtcatt gagccagagg tgaagcagat
     4381 ggttaccgtc tccggtcgta tccgtgcgga agacggcaca ctgctggcta acgcacggat
     4441 taacaaccat atcggccgaa cccgaaccga tgaaaacggc gagtttgtca tggacgtgga
     4501 taagaaatat cccactatcg attttcgcta cagtggcaat aaaacctgcg aagtggcact
     4561 ggaactcaac caggcgcgcg gtgccgtctg ggtcggtgat gtggtctgca gcggcctctc
     4621 atcgtgggcg gcggtgacgc agacaggaga agagaatgag agttaaccta ctaataacga
     4681 tgataatttt cgcgctaatc tggccagtaa ctgagctcag agcggcagtg agcaaaacaa
     4741 cctgggcgga tgcaccggca cgcgagtttg tgtttgtcga aaacaactca gacgacaact
     4801 ttttcgtcac tcctggcggg gcgctggatc cgcgcctgac cggtgccaac cgctggaccg
     4861 gtttaaaata cactggttca ggaaccatct atcagcaaag cctcggctac attgataacg
     4921 gttacaacac cggcctttat accaactgga agtttgatat gtggctggaa aattcaccag
     4981 tttcatctcc tttaactggc ttgcgctgca tcaactggta cgctgggtgt aatatgacca
     5041 ccagtcttat cctgccgcaa accaccgacg ccagtggatt ttatggcgcg acggtgacca
     5101 gcggcggcgc aaagtggatg cacggcatgt tgtcagacgc gttttaccag tatctgcaac
     5161 aaatgcccgt cggcagcagc tttacaatga ccatcaatgc ctgccagacc tctgtgaact
     5221 atgacgccag cagcggcgca cgctgtaagg atcaggcctc cggcaactgg tatgttcgca
     5281 acgtcaccca tacgaaagca gcaaatctgc ggttaataaa tacccactcg ctggcggagg
     5341 tatttatcaa cagcgacgga gtaccgactc tgggcgaagg gaacgccgac tgccggacgc
     5401 agaccatcgc cagcctttca ggattaagtt gtaagatggt caactatacc ctgcaaacaa
     5461 acggactcag caacacctca atccatatat tcccggcgat cgccaactcg tcgttagcct
     5521 cggccgtcgg ggcgtacgat atgcagttca gtctgaatgg cagttcatgg aaaccggtga
     5581 gcaataccgc ctattactac accttcaacg agatgaagag cgcagactcg atctatgttt
     5641 tcttctcgag caacttcttt aagcagatgg tgaacctcgg gatcagcgat atcaacacca
     5701 aagatctatt caactttcgc tttcagaaca ccacatcacc ggagtctggc tggtatgaat
     5761 tttctacctc caacacgctg attatcaaac cccgtgattt cagcatcagt attatctccg
     5821 atgaatatac tcagacaccg tcgcgggagg gatatgttgg cagcggcgag tcggcactcg
     5881 atttcggcta tatcgtaacc accagcggta aaacagctgc cgacgaagtg ctgatcaagg
     5941 tgaccggacc cgcgcaggtg attggcgggc gctcctattg tgtcttcagc tccgatgacg
     6001 gtaaggcgaa agtaccgttc ccggcgacac tttcctttat tacccgcaac ggagctacaa
     6061 aaacctacga tgccgggtgc gatgatagct ggcgggatat gaccgatgcg ctgtggttga
     6121 ccacaccgtg gactgatatc tctggcgaag tggggcagat ggataagacc acagtcaaat
     6181 tttcgattcc aatggataac gccatttcgc tgcgtacggt agatgataac ggctggtttg
     6241 gcgaagtcag cgcttcagga gagattcatg ttcaggcgac gtggcgtaac attaactaag
     6301 gccctgctga cagcggtctg tatgctggcg gcacctttga cacaggcgat ttcggtcggc
     6361 aatctgacat tttcgctgcc gtccgagact gactttgtca gcaaacgtgt agtgaataac
     6421 aacaaaagcg cacggatata ccgtattgcc atcagtgcta ttgatagccc gggcagcagt
     6481 gaattgcgca cccgaccggt ggatggtgaa cagcttttcg ccccccgcca gctggcgttg
     6541 caggctggtg agagcgagta ttttaaattt tactatcatg gtccacggga taaccgcgag
     6601 cgctactacc gggtctcatt tcgcgaggtc cccactcgta accagacaag acgcagccca
     6661 accggcggcg aggtcagcac ggagccggtg gtggtgatgg ataccattct ggtagtacga
     6721 ccacgtcagg ttcagtttaa atggtccttc gacaaggtga caggaacggt aagtaacact
     6781 ggcaacacat ggtttaagct actgattaag ccaggatgtg attcgaccga agaggaaggc
     6841 gatgcctggt atctacgtcc gggagacgtg gttcatcagc ctgagttacg tcagccgggg
     6901 aatcattatc tggtctataa cgacaaattc attaagatta gcgattcctg tccggctaag
     6961 cccccttcgg cggactaaga cttatccatc ggtcgaggga agaattccac ctcagagctc
     7021 caaaaatcgt ttaaatgatg tggaagcgat cgtcaatgtc acctgtaacg atgggctttc
     7081 tcatatgcac accatagtgt tcaggagaca cggaaaggta tcaggcagcc tgctaacgag
     7141 tggtttaatc acgaattagt acgtaaaatc ggtaacggct ggaaatcatt caatactcac
     7201 actatcgaaa gttccccagc caaccgcggt acgttcttac atacgatgta ccgctgttct
     7261 ctttacgatt tttagctgta ctggtgaatt atgagcaatc tgaatccatg catgacgtgt
     7321 ggtgcctgtt gtgcattttt ccgcgtctct ttttactggg ccgaagccga cgatgctggc
     7381 ggtactattc ccgccaggct cactgaacaa atatcccctt ttcaccgatg catgagcggt
     7441 accaatcaga aaaacccccg atgtattgcc cttgcaggaa ccccgggcaa aaatgcctgc
     7501 tgcacgatat ataaaaatcg atcgtccaca tgcagagaat ttgccatgtc tggtgagaac
     7561 ggagtcgtca atgaggcttg caatcgtgca agggctaaat acgggctgac accgttataa
     7621 acatacaata attaattgca ctgccccgcc agcgataata gcggggcttc gtttttcagg
     7681 ggtaacaaaa cccgatactt cttctatttg ccagcaacaa tgcctctctt ctgtagcgtt
     7741 cttcggatct acatcatcct gaagtagcgc gatatactca ctgactatct gcatcagaat
     7801 ataaaaagca atgtttttaa cctataaaaa tggcgctgta tttgcgccat ttttatcatt
     7861 caatgcatta tctgtttgag cctaaaggga tctcagggtc tggctcatga gtaattctgt
     7921 ttcgtaaatc tctgcgaata atttcaatag accagaacca gactaaatgt ccaaaaattt
     7981 cagaaacatt ctcataccac gggagatcaa acagaggtgg cgtcagtccc atgagaggga
     8041 atgaaatcat atgaacaaaa agttgggcta aagcacctgc cagtaagccc tgccagagtt
     8101 taatttttgg aaatacttca gcgaccacac aataaccgac agcaaacact atcgaaaaga
     8161 taatgtgcgt aacaccaacc cagttaaaga catgcccggc aaaggtataa acagccgcat
     8221 tgggatctgt cagccccaac cagtctcgaa gaaaaatata cggtggattg agaaaattac
     8281 gcgagcaatc aatttggcct gcagccctga ttaatgattc cgggccacac gctgcattaa
     8341 acatatccac cgggctacgt ggcggcaatg gaacttcagc cccccacttc acgaatgcgg
     8401 aaacaacgcc agcaatcagc ccaatgaatg cagcaagacc ataacgtctg cggttcggtg
     8461 gagtttgttc aaatatattc atatctaccc tgcttgtacc attatgttat acacctcttc
     8521 aggagtattc ataaaacaag gcaaatgtaa agaactgtat tgttttgtat aacaagatag
     8581 tttcctaatc gccaatgaat ataagctcca tcatttctcc ctatttttat attagaagtg
     8641 acagagattt gcagggtgat gcagagctga aaatcacagg tttccttatt ggtttttgca
     8701 tcgtacaact aaagcaataa accagctccc atcatcatca acatcccggc gaacatgaat
     8761 ttattagccg ggctcatagc tgcattcgcg ttttaagaat aatcctcctg ctgtcgccga
     8821 ctatgcttaa cgtttaaaaa agcatcagca ctctcgcaac gcactcttat tttccccttt
     8881 agaataccgg aggcctggta tgagcaacca aggcgaatac cccgaagata atcgggttgg
     8941 gaagcacgag ccgcacgatt taagtttgac ccgtcgcgat ctgattaaag tgagcgccgc
     9001 aacagcggcg accgccgtgg tttatcctca ttctacgctg gcggcaagcg ttccggcagc
     9061 tacacccgcg ccagagataa tgcccctgac actgaaggtg aacggcaaaa ccgagcagct
     9121 tgaggtggat acccgaacca cgctactgga cgctttgcgt gaaaatctgc atttgatcgg
     9181 taccaagaaa ggttgcgatc acggacagtg cggagcctgt accgtgctgg tcaatggtcg
     9241 caggcttaat gcctgcctga cgcttgcagt catgcatcag ggggccgaga tcaccaccat
     9301 tgaaggcctg ggttcgccag ataatcttca ccccatgcag gcggccttta tcaagcatga
     9361 tggcttccag tgcggctact gcacctccgg gcaaatttgc tcatcagtag cggtgctaaa
     9421 agagattcag gacggcattc ccagtcacgt cacggtcgat ttggtttccg ctccagaaac
     9481 aactgccgat gagatccgtg aacgtatgag cggcaacatc tgtcgctgtg gtgcatacgc
     9541 taacatcctt gccgccattg aagatgctgc tggggagata aaatcatgaa ggcgtttacc
     9601 tatgaacgag tgaacacccc agccgaggcg gcacttagcg ctcagcgcgt acccggcgca
     9661 aaatttatcg cgggcgggac gaatctgctg gacctgatga agctggaaat tgaaacgccc
     9721 acccacctta tcgatgtgaa cggcctcggg ctcgataaga ttgaagtgac cgacgcgggt
     9781 gggctgcgca tcggcgcact ggtacggaac accgacctgg cggctcacga gcgcgtgcgt
     9841 cgtgattacg cggtactctc ccgcgccctg ctcgctggcg catctggtca gttacgtaat
     9901 caggcaacca ccgcaggtaa tctgctccag cgcacgcgct gcccctattt ttacgacacc
     9961 aatcagccct gcaataagcg cctgcccggg agcggctgcg cggcgcttga aggctttagc
    10021 cgtcagcacg cggtggtagg cgtaagtgaa gcctgcatag ccacccatcc gagcgatatg
    10081 gcggtcgcaa tgcggttgct ggatgcggtg gtggaaacca tcacgccgga gggaaagact
    10141 cgcagtatca cactggctga tttttatcac ccactgggga aaacgccgca cattgaaacc
    10201 gccctgcttc ccggtgagct tatcgttgcg gtgacgttac ctccaccgct cggcggaaaa
    10261 catatctacc gtaaggtgcg cgatcgcgcc tcctacgcct ttgccctggt atcggtcgcg
    10321 gcgattattc agcctgacgg cagcgggcgc gtcgcgctgg gcggagtagc acataagccc
    10381 tggcgcattg aggctgcgga tgctcagcta tcccaggggg cgcaggccgt atatgacacg
    10441 ctgttcgcca gcgcccatcc caccgctgaa aacaccttta aactcctgtt ggcgaagcga
    10501 acgcttgcct ccgtactggc tgaagcgagg gcacaggcat gaaatttgat aaacccgcag
    10561 gggaaaaccc gatcgatcag ctgaaggttg tcggtcgtcc ccatgaccgc atcgacggac
    10621 cgctgaaaac taccggcacg gcacgctacg cctacgaatg gcatgaagag gcccccaacg
    10681 ccgcctatgg ctatatcgtc ggttccgcca ttgccaaagg acgcctcacc gcccttgata
    10741 cggacgccgc gcaaaaagcg ccgggcgtac tggctgtcat taccgccagt aacgccgggg
    10801 cactcggcaa aggcgacaaa aacaccgcca ggctgttagg cggccccact attgagcact
    10861 atcatcaggc cattgcgctg gtagtggccg agaccttcga acaggcgcga gcggcggcct
    10921 cgctggtgca ggcacactat cgccgtaata aaggagctta ctccctggcg gacgaaaaac
    10981 aggccgtcaa tcagccgccg gaagacacgc ccgacaaaaa cgtcggtgac tttgacgggg
    11041 ctttcacctc cgctgcggtg aagattgatg ctacctacac gaccccggac cagagccata
    11101 tggcgatgga gccgcatgcc tcgatggccg tctgggatgg aaataagctt accctctgga
    11161 cctcaaatca gatgattgac tggtgccgca ccgatctggc aaaaacgctg aaagttcccg
    11221 tggagaatgt gcgtattatc tccccgtata tcggcggagg gtttggcggc aagctgttcc
    11281 tgagaagcga tgcactgctg gcggcgctcg ccgcccgagc ggtgaaacgt ccggttaaag
    11341 tgatgctccc ccgccccact
//
LOCUS       DAZKIV010000149        11348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZKIV010000149 DAZKIV010000000
VERSION     DAZKIV010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.149"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..831)
                     /gene="cobB"
                     /locus_tag="ACHOT6_003855"
     CDS             complement(10..831)
                     /gene="cobB"
                     /locus_tag="ACHOT6_003855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFP9949005.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(847..1758)
                     /gene="nagK"
                     /locus_tag="ACHOT6_003856"
     CDS             complement(847..1758)
                     /gene="nagK"
                     /locus_tag="ACHOT6_003856"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFP9949006.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIQGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(1787..3031)
                     /gene="lolE"
                     /locus_tag="ACHOT6_003857"
     CDS             complement(1787..3031)
                     /gene="lolE"
                     /locus_tag="ACHOT6_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFP9949007.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(3031..3732)
                     /gene="lolD"
                     /locus_tag="ACHOT6_003858"
     CDS             complement(3031..3732)
                     /gene="lolD"
                     /locus_tag="ACHOT6_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFP9949008.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(3725..4924)
                     /gene="lolC"
                     /locus_tag="ACHOT6_003859"
     CDS             complement(3725..4924)
                     /gene="lolC"
                     /locus_tag="ACHOT6_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFP9949009.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            5186..6259
                     /locus_tag="ACHOT6_003860"
     CDS             5186..6259
                     /locus_tag="ACHOT6_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFP9949010.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            6387..9833
                     /gene="mfd"
                     /locus_tag="ACHOT6_003861"
     CDS             6387..9833
                     /gene="mfd"
                     /locus_tag="ACHOT6_003861"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFP9949011.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANCPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            9977..10939
                     /gene="ldtC"
                     /locus_tag="ACHOT6_003862"
     CDS             9977..10939
                     /gene="ldtC"
                     /locus_tag="ACHOT6_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFP9949012.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRAHYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(11022..11279)
                     /gene="bhsA"
                     /locus_tag="ACHOT6_003863"
                     /gene_synonym="ycfR"
     CDS             complement(11022..11279)
                     /gene="bhsA"
                     /locus_tag="ACHOT6_003863"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFP9949013.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         2729 a         3080 c         2885 g         2654 t
ORIGIN      
        1 cttcctgctt tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc
       61 cgggccgtaa tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc
      121 cacggtgtgc gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg
      181 cccggaagta ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc
      241 catgccgagt ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg
      301 gcaacaatgg catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact
      361 ttgcgaacaa cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc
      421 gcgttcatgc aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagggcgtc
      481 ttgcagttta gcaagcgcaa gatgcgcggc gttaggctga atttctggct gctgcagctg
      541 tcgacgacgg gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg
      601 cgttgccaca tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg
      661 aatacctgat tccgcagaaa tccctgcccc tgtcagtacg agtactcttg gtttttccat
      721 tgcttccggc accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg
      781 gcgtttattt ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc
      841 tgttgtttaa tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg
      901 gcgcgcgcgt tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc
      961 cgccagttgc gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc
     1021 agggtcaaca atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc
     1081 aacgtgcgcc cttgcctgct catcgccttg atcataaagc gcaataattt cgggagcctg
     1141 caacggttga tgataatagt gttgatacag ccacgcaaaa ccgcgaccag acagataatt
     1201 ttcaatgcag ccatgctgac cacagccgca gcggcgtaac gggaaatcca accccatcat
     1261 ggttaacgca tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc
     1321 ggtaatcggt ttgccgttga aaatcagccc gccgccaacg ccggtgccga gaatcaaccc
     1381 catcaccagt ggatattgag taaattcatc atcccaggct tctgaaaggg caaaacagtt
     1441 ggcatcgtta tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg
     1501 tttaccgctg gcagcgggga cattggcggc atacagcgta ccatcttctg tttccggcat
     1561 tccctgaata ccgatgccga cagaaccttt acagccaaaa cgttggtcag cttcagctac
     1621 cagctcacac actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt
     1681 ttcccactgc aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat
     1741 atcaaacccg taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa
     1801 ggactcgcgc agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac
     1861 tcagcaacaa tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca
     1921 ggaaatcaat aaaatagata tcgctggaga ggaactgatg accgatcaac ttttcaatcc
     1981 actcaataat cggggtaagt tgcagtgaaa ccaccacacc gataatcacc ccacacaggc
     2041 tgccgaacag ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt
     2101 tcgcccccag cgttcttaat actgcgatat cgccactctt gtctttcacc gccatcacta
     2161 aggtggagac gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa
     2221 tggcgcggat catctggata tcgcgataca tatagccgta agtaccaatc caacttttaa
     2281 tataaacata gctgttggtc acttcacccg catcgcgtac cagcttattg gcgttgaaaa
     2341 catccgtcat tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg
     2401 catccgccag cgggatcatg gcaaaactgt gatcgagttg accactcaac tgcaaaatac
     2461 cggcaacgtg caaacgcaca cgttttggct gcatcagttt atgctcggga ttcgagttgg
     2521 ggatcataat cgacacccaa tccccctgct tcactttcag cgcatccgcc acgcctttgc
     2581 cgatgataat ttgctgttcg cccgctttaa aattgcgcca cgcatcgccc tggacaaacg
     2641 agggtaatgc gctcagacgc tgttcctgtt gcgggttaac gcccttcacc tggattgcgc
     2701 gaagattcgc gccactttcc accagcccgg tgaaattgat atacggcgcg gcagcggcaa
     2761 tacccggcac tttttgcacg tgatccagtg cttcctgcca gttagtccac ggctgatcca
     2821 ccgcctcgat ttcaccatgc ggcaccaccg ccagaatacg gttgttcagt tcgcgttcaa
     2881 agccgttcat cgcgcttaag ccgacgatca ataccgccac gccgagggca atgccaatgg
     2941 tagaaataac ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga ctaaaacgca
     3001 ggccaattaa taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc
     3061 cgtcagacga ccatcacgca tttctaactg gcggctcata cgtttcgcca gttgcaggtc
     3121 gtgagtaacc accaggaagg cggtgccctg caagcgattc aattccccaa gcaactggaa
     3181 gatgctgtcg gcgttacgcg catcgaggtt accggtaggt tcatccgcca gtaccaggcg
     3241 cgggttattc accagcgcac gggcaatcgc cacacgctgg cgttcgccgc cagaaagttc
     3301 agatgggcgg tgattcgcac gatgatccag ccccaccgct tttaacatct caagtgcacg
     3361 gctgttgatt tcagcgggct ttttcttgcc aatcagcagc ggcatagcca cgttttccag
     3421 ggcggtaaaa tccggcagca ggtggtgaaa ctgataaata aagcccagct tctggttgcg
     3481 cagctctgct ttcgccgccg aagacagttt gctcatcggt tgaccgttaa agatcacatc
     3541 gccggaggtt ggcgtatcca gcccgcccaa caagtgcagc aaggtgcttt taccggaacc
     3601 agagctaccg acgatcgcca tcatttcacc ttcgccgacg ctgaaactga cattgtgcag
     3661 cacatcggtt tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag
     3721 gatcttattc ataacgtaaa gcctcagcgg gttgagtggc ggcagcgcgc catgaagggt
     3781 aaagcgtaga cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag
     3841 gttcgatagc gaccggcagc gccgcgccat caagcaggac gccgattatc ggcatcagat
     3901 tatttaactg gctggcaagc agggcgccaa gcgccgctcc gaggatcgca ccgataatcc
     3961 cggcgctggc cccttgcacc ataaagacca tcatgatttg tcgcggagtt aagccttgcg
     4021 tttgcaggat cgctacttcg ccctgcttct ccattaccat cagccctagt gaggtaataa
     4081 tgttaaacgc cgcaacggcg acaatcaggc tcagcagtaa acccatcata tttttttcca
     4141 tgcgtacggc ctggaacaac tcgcctttac gatcacgcca gtcctgccat ttgctgcctt
     4201 caggcagttt ttgctgactt aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc
     4261 agccggtaat attgcctgcc ggataacgca tcagacgcga ggcatcctca atattcacca
     4321 gcatttcatt gccatcgact tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc
     4381 gctgacttgg aatacgcccc atcggcgtga actggctggc agatggtacc atcatgcgga
     4441 tttgatcacc gcgattaacg cctagctgtg aggcaagttg ttcgccgagg atgacattat
     4501 atttccccgg ctcgaggtca gtttgtttca cattgaccag atacggcgtt agtggatctt
     4561 tttgcgccgg gtcgataccg agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta
     4621 ccacatcacc ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt
     4681 ccgggagttg ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca
     4741 ggccaaggat gttgttttgc agctcgcgct caaagccgtt catcactgac aatactgtga
     4801 ccagcgccat caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga
     4861 agcgatccgc tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt
     4921 acatgaaatc cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg
     4981 gtaaacgtct gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat
     5041 cggctaaatt gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat
     5101 ccaatacaga cagataccca ttacacctcg ggtcataacc cgcaggatca accgcatgta
     5161 aattaaaaaa gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa
     5221 agggttatgt atttgtctgg tggtgattta tcactcggtc attacctttt atccgcatct
     5281 gaccactttt cagcatccgt tatcggaagt cctgagcaaa tgctggatct atttcaatct
     5341 ttaccttgcc ccctttcgta tgccggtttt tttctttatt tccggctatt tgattcgccg
     5401 ctatatcgac agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg
     5461 ggtgctggcg ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct
     5521 ggcacctgag cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga
     5581 gttcctgcac gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt
     5641 ctatttcgtg gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt
     5701 actgctgagt gtggcggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat
     5761 ccgcaatttg ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt
     5821 taaagaggtg ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt
     5881 cggtgcctgg ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat
     5941 gaagctgttt tatcaatacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt
     6001 gattggctcc aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag
     6061 cttaactctg cttgcgcaaa tgaacgcagc acgctggtcg ccgcaagtcg aactgacact
     6121 cctgctggtt tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag
     6181 aaaactttca cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc
     6241 cgtcagttac tcccgctaag ccatcgcccc agccggggcg attcaatttg ctaaaccatg
     6301 tcgattacgg ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat
     6361 atcctaacga gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca
     6421 aagcgggtga gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag
     6481 cggaaattgc cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg
     6541 ctctgcgttt gcatgatgaa atcagccagt tcaccgatca gatggtgatg aatctggcgg
     6601 actgggaaac tcttccctac gacagttttt cgcctcatca ggacattatc tcctcgcgcc
     6661 tttccaccct ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata
     6721 cgcttatgca gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa
     6781 aaggtcagcg cctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc
     6841 atgttgacca ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct
     6901 tcccgatggg gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc
     6961 tgcgggtgtt tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc
     7021 tgcccgcgca cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc
     7081 gcgatacctt cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca
     7141 cattacctgc cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc
     7201 tgttcagtta tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg
     7261 ccgaacgttt ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc
     7321 gcccgctgtt gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga
     7381 aaaactggcc ccgggtgcag ctaaaaactg aacatttacc gacaaaagcc gcgaatgcca
     7441 atttaggttt ccagaaactg ccagacctgg ccgttcaggc gcaacaaaaa gcgccgctgg
     7501 atgcgctgcg taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg
     7561 aaggtcgccg tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca
     7621 ttatgcgtct tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac
     7681 atggttttgt cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg
     7741 aacgtgttgc ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc
     7801 gtaaccttgc ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc
     7861 gctatgccgg aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca
     7921 cctatgccaa cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt
     7981 acgcaggtgg cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac
     8041 gcgcgcggca gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct
     8101 acgcgcaacg cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt
     8161 tgttctgcga cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg
     8221 tacttagcga catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct
     8281 ttggtaaaac agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg
     8341 tggcggtgtt ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc
     8401 gtttcgccaa ctgtccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc
     8461 agacgcaaat ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca
     8521 aactgctgca aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac
     8581 accgcttcgg ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc
     8641 tgacgcttac tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg
     8701 acctgtcgat tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg
     8761 agtatgacag cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg
     8821 tttattatct ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac
     8881 tggtgccaga agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac
     8941 gggtgatgaa tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg
     9001 aaaccgggat cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg
     9061 gtctggcgca gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg
     9121 catggctgct gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag
     9181 cgattgcctc gctggaagat ctcggtgcag gctttgcgct ggcaacgcac gatctggaga
     9241 tccgcggcgc gggtgaactg cttggcgaag aacaaagtgg ctcaatggaa accatcggtt
     9301 tctcgctgta tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc
     9361 cgtcgctgga agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat
     9421 tgccagatga tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca
     9481 gcgccaaaac ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc
     9541 tgctgccgga tccggcgcgt accctgctgg atgttgcccg tctgcgccag caagcgcaga
     9601 aactggggat caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga
     9661 agaatcacgt taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc
     9721 gccttgatgg tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta
     9781 tcgaatgggt acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa
     9841 tcgggggcgt ttctgcgccc ccatctgcaa catttacaaa ttctttgcac ttccctgcac
     9901 tatccgacac tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat
     9961 ttattatgga tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac
    10021 gttcgccgct gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc
    10081 agcgggcagt cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc
    10141 tctggaagcc atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc
    10201 tggcgttgat ccttacgtac cgcgcgcggg tagcgtgtta acgatcccgt tgcaaaccct
    10261 acttccagat gcgccgcgcg aaggcattgt gatcaatatt gcggagctgc gtctctatta
    10321 ctacccgccg ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg
    10381 tgacacgctg acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg
    10441 gacgccaacg gcaaacatcc gcgcccatta taaagcacag ggaattgagt tgcctgcggt
    10501 agtgccggct ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg
    10561 cggcgtttat ttgcttcatg gtacgaacgc cgatttcggc attggcatgc gggtaagttc
    10621 tggctgtatt cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg
    10681 caccaaagtg aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg
    10741 tctggttgaa gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc
    10801 aattacgctg aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt
    10861 gatgcaacat gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt
    10921 gagcccacaa actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg
    10981 ggaaaaaatc agcagtagca ggcattaatg agggttaatg cttatttata aattactgct
    11041 gttccatgga gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc
    11101 tcatccgctt tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca
    11161 ctgattgtac cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag
    11221 ctggcaaatg acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata
    11281 atagtggcct tatgcagatg aatgacgtcg caagcaatta cttaacgatc gataactaaa
    11341 tgatagat
//
LOCUS       DAZKIV010000150        10944 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZKIV010000150 DAZKIV010000000
VERSION     DAZKIV010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.150"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            296..1438
                     /gene="yghO"
                     /locus_tag="ACHOT6_003864"
     CDS             296..1438
                     /gene="yghO"
                     /locus_tag="ACHOT6_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFP9949014.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1445..2209)
                     /gene="glcC"
                     /locus_tag="ACHOT6_003865"
     CDS             complement(1445..2209)
                     /gene="glcC"
                     /locus_tag="ACHOT6_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFP9949015.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2460..3959
                     /gene="glcD"
                     /locus_tag="ACHOT6_003866"
     CDS             2460..3959
                     /gene="glcD"
                     /locus_tag="ACHOT6_003866"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFP9949016.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3959..5011
                     /gene="glcE"
                     /locus_tag="ACHOT6_003867"
     CDS             3959..5011
                     /gene="glcE"
                     /locus_tag="ACHOT6_003867"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFP9949017.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSQLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            5022..6245
                     /gene="glcF"
                     /locus_tag="ACHOT6_003868"
     CDS             5022..6245
                     /gene="glcF"
                     /locus_tag="ACHOT6_003868"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFP9949018.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6250..6654
                     /locus_tag="ACHOT6_003869"
     CDS             6250..6654
                     /locus_tag="ACHOT6_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFP9949019.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6676..8847
                     /gene="glcB"
                     /locus_tag="ACHOT6_003870"
     CDS             6676..8847
                     /gene="glcB"
                     /locus_tag="ACHOT6_003870"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFP9949020.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAVVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9203..10885
                     /gene="glcA"
                     /locus_tag="ACHOT6_003871"
     CDS             9203..10885
                     /gene="glcA"
                     /locus_tag="ACHOT6_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFP9949021.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2550 a         2741 c         3060 g         2593 t
ORIGIN      
        1 atgagatagt attttcatcg gtgtgaaaat gaacattgat ttaaataatg agtttaagtt
       61 tttgttttta ttgtgaatga tatttaattt atgaatatat taagaagaag ccttgtccgg
      121 tcgccttaaa tcgacaaaag gcattgaggc gcttctgtgt agtctctaac cttatcataa
      181 tcattaataa atactattta ttctttgttt gtattcattg ggttgattat taaggtgtcg
      241 ttcgttgctg ttgcaacctg tttttcaccg ttaattattt gttatccaca gagatatgga
      301 atgcgatctg ctgatgatta aaattgaaaa agtaattaat aaaaatgacc ttaaggcatt
      361 tatcgctttt ccgtcatcac tttatcccga cgatccaaac tggatacccc ctttattcat
      421 agaacgcaac gagcatttgt ctgcgaaaaa tccggggacg gaccatatca tctggcaggc
      481 gtgggtggca aaaaaagcgg ggcagatagt tgggcgtatt accgcgcaaa tagatacctt
      541 gcaccgtgag cgttacggtg aagataccgg tcatttcggc atgattgacg ccattgatga
      601 tccgcaggtt tttgccgcgt tgtttggcgc agcggaagcg tggttgaagt cacaaggtgc
      661 aagtaagatc agcggtcctt tcagcctgaa tatcaatcag gaaagcggat tactgattga
      721 aggttttgac acaccaccct gtgcgatgat gccacacggc aaaccgtggt atgccgcgca
      781 tattgaacaa ctgggttatc acaaaggcat tgatttactg gcgtggtgga tgcagcgaac
      841 cgatctcact ttctctccgg cgctaaaaaa actgatggat caggtgcgca aaaaggtgac
      901 cattcgctgc atcaatcgtc agcggtttgc cgaagaaatg cagatcctgc gtgagatttt
      961 caactctggc tggcagcaca actggggatt tgtgccgttt accgaacatg aattcgcgac
     1021 catgggcgat caacttaaat atctggtgcc ggacgatatg atctatatcg ctgagattga
     1081 ttctgcaccc tgcgcgttta ttgtcggctt gccgaacatc aacgaggcga ttgccgatct
     1141 gaacggatcg ctctttccct tcggctgggc aaaattgctg tggcgtttga aagtcagcgg
     1201 tgtgcgaact gcgcgagtac cgctgatggg cgtgcgcgac gagtatcagt tcagccgcat
     1261 cggcccggtg attgcgctgt tattgattga agccttacgc gatccgtttg cccgccggaa
     1321 gatcgatgcg ctggagatgt catggatcct cgaaaccaat acaggcatga ataacatgct
     1381 ggaacgcatt ggggcggagc cgtacaagcg ttaccgattg tatgaaaagc agatttaata
     1441 ccagctaact caggttcatc tccagcggca ccgccgagcg aatcaaatgg tggccttcca
     1501 gctcgatatc gtggagattc tttttcacgg tccgcacatg atcgcgtgct gcgcgctggg
     1561 cgacgtgcgg cagccgctgc aacaccgcgt tgtagatcct cgcatgctgg cgatcgatct
     1621 gctttttttg ctgtggtcga tggtagagat tatttacgct ggcaaacact gaattaaaca
     1681 tcagatcggt caatgattgc agcgtaaaca ccagcacctg attgtgagaa gcctgacaaa
     1741 tggcgagatg gaaagcgtga tccaactgcg catgttcgat cagcgaaatc tctttgttgt
     1801 tctcactggc ggcgagcatt ttttcataac agcgggttat cacaacaaaa tcagcctgcg
     1861 ttcccagcgt tgccgccagc cttgccgatt cgccctccag taatgcgcga acgtcgagca
     1921 gatcgtacag cgttcgcggc tgcgtactga acagatggat cagcgggctg gtgtcctgca
     1981 cccgattaag ccgtgcgaca cgagaatcgc gaccctgcgc cgtttcaata atcccgcgcc
     2041 cgcgcagcac ggtcagccct tcacgcagtg cggagcgtga gaagccgagc ttttcacaca
     2101 gtcgacgttc cgagggaagt ggctgaccga ccttcagtac gccgtcgata attaaccgtt
     2161 cgatactctc tgcaaccact tcgcaaatag ggcgacgttc atctttcatt cccggacctc
     2221 gtgcacaggt aggaccaatt tttctgttct taactcgcaa aacacgcaca tcacgtaagt
     2281 gattatgtta catcaattta acattgagtt aaccaaggca aggtcacaga gctggaaaaa
     2341 aaatggtctg accggtagct aaagagatag acgaaaacga aaagcccgct taataactgt
     2401 tcacagaagc agcgcgcaaa aatcagctgc cacacaacac aacaaagcga agcctactca
     2461 tgagcatctt gtacgaagag cgtcttgatg gcgctttacc cgatgtcgac cgcacatcgg
     2521 tactgatggc actgcgtgag catgtccctg gacttgagat cctgcatacc gatgaggaga
     2581 tcattcctta cgagtgtgac gggttgagcg cgtatcgcac gcgtccatta ctggttgttc
     2641 tgcctaagca aatggaacag gtgacagcga ttcttgctgt ctgccatcgc ctgcgtgtac
     2701 cggtggtgac ccgtggtgca ggcaccgggc tttctggtgg cgcgctgccg ctggaaaaag
     2761 gtgtgttgtt ggtgatggcg cgctttaaag agatcctcga cattaacccc gttggtcgcc
     2821 gcgcgcgcgt gcagccaggc gtgcgtaacc tggcgatctc ccaggccgtt gcaccgcata
     2881 atctctacta cgcaccggac ccttcctcac aaatcgcctg ttccattggc ggcaacgtgg
     2941 cagaaaatgc cggcggcgtc cactgcctga aatatggtct gaccgtacat aacctgctga
     3001 aaattgaagt gcaaacgctg gacggcgagg cgctgacgct gggatcggac gcgctggatt
     3061 cacctggttt tgacctgctg gcgctgttca ccggatcgga aggtatgctc ggcgtgacca
     3121 ccgaagtgac ggtaaaactg ctgccgaagc cgcccgtggc gcgggttctg ttagccagct
     3181 ttgactcggt agaaaaagcc ggacttgcag ttggtgacat catcgccaat ggcattatcc
     3241 ccggcgggct ggagatgatg gataacctgt cgatccgcgc ggcggaagat tttattcatg
     3301 ccggttatcc cgtcgacgcc gaagcgattt tgttatgcga gctggacggc gtggagtctg
     3361 acgtacagga agactgcgag cgggttaacg acatcttgtt gaaagcgggc gcgactgacg
     3421 tccgtctggc acaggacgaa gcagagcgcg tacgtttctg ggccggtcgc aaaaatgcgt
     3481 tcccggcggt aggacgtatc tccccggatt actactgcat ggatggcacc atcccgcgtc
     3541 gcgccctgcc tggcgtactg gaaggcattg cccgtttatc gcagcaatat gatttacgtg
     3601 ttgccaacgt ctttcatgcc ggagatggca acatgcaccc gttaatcctt ttcgatgcca
     3661 acgaacccgg tgaatttgcc cgcgcggaag agctgggtgg gaagatcctc gaactctgcg
     3721 ttgaagttgg cggcagcatc agtggggaac atggcatcgg gcgagaaaaa atcaatcaaa
     3781 tgtgcgccca gttcaacagc gatgaaatca cgaccttcca tgcggtcaag gcggcgtttg
     3841 accccgatgg tttgctgaac cctgggaaaa acattcccac gctacaccgc tgtgctgaat
     3901 ttggtgccat gcatgtgcat cacggtcatt tacctttccc tgaactggag cgtttctgat
     3961 gctacgcgag tgtgattaca gccaggcgct gctggagcag gtgaatcagg caattagcga
     4021 taaaacgccg ctggtgattc agggcagcaa tagcaaagcc tttttaggtc gccctgtcac
     4081 cgggcaaacg ctggatgtgc gttgtcatcg cggcattgtt aattacgacc cgaccgagct
     4141 ggtgataacc gcgcgtgccg gaacgccgct ggtggcgatt gaagcggcgc tggagagcgc
     4201 cgggcaaatg ctcccctgcg agccgccgca ttatggtgaa gaagccacct ggggcgggat
     4261 ggtcgcctgc gggctggcgg ggccgcgtcg cccgtggagc ggttcggtcc gcgattttgt
     4321 cctcggcacg cgcatcatta ccggcgctgg aaaacatctg cgttttggtg gcgaagtgat
     4381 gaaaaacgtt gccggatacg atctctcaca gttaatggcc ggaagctacg gttgtcttgg
     4441 cgtgctcact gaaatctcga tgaaagtgtt accgcgaccg cgcgcctccc tgagcctgcg
     4501 tcgggaaatc agcctgcaag aagccatgaa cgaaatcgcc cagtggcaac tccagccgct
     4561 acccattagt ggcttatgtt acttcgacaa tgcgttgtgg atccgccttg agggcggcga
     4621 aggatcggta aaagcggcgc gtgaactgct ggggggcgaa gaggttgcgg gtcagttctg
     4681 gcagcaattg cgtgaacaac aactgccgtt cttctcgtta ccaggtacct tatggcgcat
     4741 ttcattaccc agcgatgcgc cgatgatgga tttacccggc gagcaactga tcgactgggg
     4801 cggagcgtta cgctggctga aatcgacagc cgaggacaat caaatccatc gcatcgcccg
     4861 caacgctggc ggtcatgcga cccgctttag tgccggagat ggtggctttg ccccgctatc
     4921 ggctccttta ttccgctatc accagcagct taaacagcag ctcgaccctt gcggcgtgtt
     4981 taaccccggt cgcatgtacg cggaactttg aggagcaggc tatgcaaacc caattaactg
     5041 aagagatgcg gcagaacgcg cgcgcgctgg aagccgacag catcctgcgc gcctgtgttc
     5101 actgcggatt ttgtaccgca acctgcccaa cctatcagct tctgggcgat gaactggacg
     5161 ggccgcgcgg gcgcatctat ctgattaaac aggtgctgga aggcaacgaa gtcacgctta
     5221 aaacacagga gcatctcgat cgctgcctca cttgccgcaa ttgtgaaacc acctgtcctt
     5281 ctggtgtgcg ctatcacaat ttgctggata tcgggcgtga tattgtcgag cagaaagtga
     5341 aacgcccact gccggagcga atactgcgcg aaggattgcg ccaggtagtg ccgcgtccgg
     5401 ctgtcttccg tgcgctgacg caggtagggc tggtgctgcg accgttttta ccggaacagg
     5461 tcagagcaaa actgcctgcc gaaacggtga aagctaaacc gcgtccgccg ctgcgccata
     5521 agcgtcgggt tttaatgctg gaaggctgcg cccagcctac gctttcgccc aacaccaacg
     5581 cggcaactgc gcgagtgctg gatcgtctgg ggatcagcgt catgtcagct aacgaagcag
     5641 gctgttgtgg cgcggtggac tatcatctta atgcgcagga gaaagggctg gcacgggcgc
     5701 gcaataatat tgatgcctgg tggcccgcga ttgaagcagg tgctgaggca attttgcaaa
     5761 ccgccagcgg ttgcggcgcg tttgtcaaag agtacgggca gatgctgaaa aacgatgcgt
     5821 tatatgccga taaagcgcgt caagtcagtg aactggcggt cgatttagtc gagcttctgc
     5881 gcgaggaacc gctggaaaaa ctggcagttc gcggcgataa aaaactggcc ttccactgcc
     5941 cgtgtaccct acaacatgcg caaaagctga acggcgaagt ggaaaaagtg ttgcttcgtc
     6001 ttggatttac cttaacggac gttcccgaca gccatctgtg ctgcggttca gcgggaacat
     6061 atgcgttaac gcatcccgat ctggcacgcc agctgcggga taacaaaatg aatgcgctgg
     6121 aaagcggcaa accggaaatg atcgtcaccg ccaacattgg ttgccagacg catctggcga
     6181 gcgccggtcg tacctctgtg cgtcactgga ttgaaattgt agaacaagcc cttgaaaagg
     6241 aataacaaaa tgaaaactaa agtcattctt agccagcaaa tggcgagtgc aattattgcc
     6301 gcaggtcagg aagaggcgca gaaaaataac tggtctgttt ccattgctgt tgccgatgac
     6361 ggcggtcatc tgctggcgtt aagtcgcatg gacgattgcg cgccgattgc ggcttacatc
     6421 tcccaggaga aagcgcgtac cgccgcgctg gggcgtcgtg aaactaaggg ctatgaagag
     6481 atggtgaaca acggacgtac cgcgttcgtg actgcgccgt tattaacgtc gctggaaggc
     6541 ggcgtaccgg ttgttgttga tgggcaaatt attggtgccg tgggcgtttc tggtttaacc
     6601 ggagcacagg atgcgcaggt cgcgaaagcg gcagcagcgg tgttggcgaa ataagcgaaa
     6661 acgaggagat aaacaatgag tcaaaccata acccagggcc gtttacgcat tgacgccaat
     6721 tttaaacgtt ttgtggatga agaagtttta ccgggaacag ggctggacgc tgcggcgttc
     6781 tggcgcaatt ttgatgagat cgttcacgat ctggcaccag aaaatcgtca gttgctggca
     6841 gaacgcgatc gcattcaggc ggcgcttgat gagtggcatc gcagcaatcc ggggccggta
     6901 aaagataaag cggcctataa atctttcctg cgtgaactgg gctacctggt gccgcaaccg
     6961 gagcgcgtga cggtggaaac cacgggcatt gacagcgaaa tcaccagtca ggcggggcca
     7021 cagctggtgg ttccggcaat gaacgcccgc tacgcgctga acgcggcgaa cgctcgctgg
     7081 ggctcactgt acgatgcgtt atacggcagc gacatcatcc cgcaggaagg ggcggtggtc
     7141 agcggctacg atccgcaacg cggtgagcag gttatcgcct gggttcggcg tttcctcgat
     7201 gaatctctac cgctggaaaa cggcagctat caggatgtgg tggcgtttaa ggtggtcgat
     7261 aaacaattac gcatccagtt gaaaaatggt aaagaaacca cgttacgtac cccggcgcag
     7321 tttgtcggtt accgtggcga tgccgctgcg ctgacctgca ttttgctgaa aaataacggc
     7381 ctgcatattg aactgcaaat tgatgccaac gggcggattg gcaaagacga tccggcgcac
     7441 atcaacgatg ttatcgtcga agctgctatc agtaccattc tcgactgcga agattcggtc
     7501 gcggcggttg atgcggaaga taaaatcctg ctgtaccgta acctgctggg cctgatgcag
     7561 gggactctgc aagagaaaat ggagaaaaac ggtcgacaaa tcgtgcgtaa actgaatgac
     7621 gatcgtcatt acaccgccgc cgatggctct gaaatttctc tgcacggacg ctcgctgctg
     7681 tttatccgca acgtgggtca tttgatgacc attcctgtga tttgggacag cgaaggcaat
     7741 gaaatcccgg aaggcattct tgatggcgtc atgactggcg cgattgccct ctatgattta
     7801 aaagtgcaga aaaactcgcg cactggcagc gtctatattg tgaaaccgaa aatgcacggt
     7861 ccgcaggaag ttgcgttcgc caacaaactg tttacccgca ttgagacaat gctcggtatg
     7921 gcaccgaata ccctgaaaat gggcattatg gatgaagaac gtcggacctc gctgaacttg
     7981 cgtagctgta tcgctcaggc gcgtaaccgc gtggcgttca tcaataccgg tttcctcgac
     8041 cgtaccggcg atgaaatgca ttcggtgatg gaagctggcc cgatgctgcg taaaaaccag
     8101 atgaaatcga cgccttggat caaagcctac gaacgtaata acgtgctttc cggcctgttc
     8161 tgtgggctgc gcggtaaagc gcaaatcggt aaaggcatgt gggcaatgcc ggacctgatg
     8221 gcagacatgt acagccagaa gggcgaccaa ctgcgtgccg gggcaaacac agcctgggtt
     8281 ccgtcgccaa ctgccgctac gctccatgcg ctgcactacc accaaaccaa cgtacagagc
     8341 gtgcaagcca acattgccca gaccgagttc aatgctgaat ttgaaccgct gctggacgat
     8401 ctgctgacta ttccggtggc tgaaaacgct aactggtcgg tggaagagat ccaacaagag
     8461 ctggataaca acgtgcaggg gattctgggg tacgtggtgc gctgggtgga gcaggggatt
     8521 ggttgttcaa aagtgccgga tattcacaat gtggcgctga tggaagaccg cgcaacgctg
     8581 cgtatctcca gccagcatat cgccaactgg ttacgtcacg gtattctgac caaagaacag
     8641 gtgcaggcgt cgctggagaa tatggcgaaa gtggttgatc agcaaaacgc tgacgatccg
     8701 gcttatcgtc cgatggcggg gaatttcgct aactcgtgtg cttttaaagc tgccagcgat
     8761 ttaatcttcc tcggcgtgaa acagccaaac ggctataccg aaccgttatt acacgcctgg
     8821 cgtttacgcg aaaaagaaag tcattaatag cgtcaaaaaa agccccggca agttttattg
     8881 tcggggctat gatagtaact gcggtagtac atttatcccg gaaataaccg gagcgattat
     8941 cagggtttaa caatattaaa tacccgtagc gcattactag cgggcctata acaacaatat
     9001 ggacatctta acgatactct gcgcctgcgt gtgcagaaca gaaaccctta cccgatatac
     9061 ttgttatgcc agaggtagca attgcgttag cggcgtactg ttgctgtaaa gtataacgcc
     9121 tatattttac agttcagtcc tcttttgctt attacaacct taagtatatc aagcatataa
     9181 agataataag agactgaaca atatggttac ctggacccaa atgtatatgc cgatgggagg
     9241 actggggcta tccgctctgg tcgccctgat cccgataata ttcttcttcg ttgcactcgc
     9301 ggtattacgt ctgaaaggac atgtcgctgg agcaataacc cttatattat ctatcctgat
     9361 tgcaatattc gcctttaaaa tgccgattga tatggctttt gctgctgcgg gctatggctt
     9421 tatttatgga ttatggccta ttgcgtggat tattgtcgcg gcggtgttcc tctataaatt
     9481 aaccgttgcc agcgggcagt tcgatattat ccgcagctcg gttatctcca tcaccgacga
     9541 tcagcgtttg caggtgttac tgattggttt ctcctttggt gcgttgctgg aaggagcggc
     9601 tggctttggt gctccggtgg cgattaccgg tgcgctgctg gtgggcctgg gcttcaaacc
     9661 gttatacgcg gcggggttgt gtctgattgc caacactgct ccggtggcgt ttggtgcgtt
     9721 gggcgtgccg attctggtcg ccggtcaggt aacgggaatc gatccgttcc acattggcgc
     9781 aatggcggga cgccagttac cgttcctgtc ggttcttgtg ccgttctggc tggtagcaat
     9841 gatggacggc tggaaagggg tgaaagagac gtggccagcg gcgctggttg ctgggggaag
     9901 ctttgctgtc actcagttct ttacctctaa ctatattggt ccggaactgc cggatattac
     9961 ttcggcgctg gtgagtatcg tctcactcgc tttattcctt aaagtctggc ggccgaaaaa
    10021 taccgaaacg gcaatcagca tggggcaatc cgcaggtgcg atggtggtaa ataagccatc
    10081 ttctggcggt cccgtgcctt cagaatatag tctggggcaa atcattcgtg cgtggtcacc
    10141 gtttttaatc ttaacggtgc tggtcaccat ctggaccatg aagccgttta aagcgttatt
    10201 tgctccgggc ggcgcgtttt attcactggt gattaatttc cagatccctc atttgcatca
    10261 acaagtgttg aaagcggcac ccattgtcgc ccaaccaacg ccaatggatg cggtgtttaa
    10321 attcgaccct ctctcggctg gcggcaccgc tatttttatt gcggcgatta tctctatctt
    10381 catcctcggc gtgggcatca agaaagggat tggcgtcttt gccgaaacgc taattagtct
    10441 gaagtggccg atattgtcga ttggcatggt gctggcgttc gccttcgtca ccaactattc
    10501 cggcatgtcc accacgttgg cgctggtact ggcaggtaca ggcgtgatgt tcccgttttt
    10561 ctcaccgttc ctcggctggc tgggggtatt ccttaccggc tcggacactt cttcaaacgc
    10621 cctgtttggt tcactgcaat cgaccacggc gcagcaaatc aacgtctctg acactctgct
    10681 ggtggcagca aataccagcg gcggcgtaac tggcaagatg atctccccgc aatctatcgc
    10741 cgtggcctgc gccgcgacgg gcatggtggg ccgagaatct gaactattcc gctacaccgt
    10801 gaagcacagt ctgatttttg ccagcgttat cggcgttatc accctgctgc aggcgtatgt
    10861 gtttaccggg atgttagtct cgtaaatcaa taaagccgga tgatcgttaa tatcatccgg
    10921 cttcatttac attattctgt ctta
//
LOCUS       DAZKIV010000151        10931 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZKIV010000151 DAZKIV010000000
VERSION     DAZKIV010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.151"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            370..483
                     /locus_tag="ACHOT6_003872"
     CDS             370..483
                     /locus_tag="ACHOT6_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFP9949022.1"
                     /translation="MNSILIITSLLIIFSIFGHALIKLGIGISNNPDKTDV"
     gene            552..785
                     /gene="ydfK"
                     /locus_tag="ACHOT6_003873"
     CDS             552..785
                     /gene="ydfK"
                     /locus_tag="ACHOT6_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFP9949023.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLKDNQNLNGKV"
     gene            1165..1755
                     /locus_tag="ACHOT6_003874"
     CDS             1165..1755
                     /locus_tag="ACHOT6_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9949024.1"
                     /translation="MSRIFAYCRISTLDQTTENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMQVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(1853..2428)
                     /locus_tag="ACHOT6_003875"
     CDS             complement(1853..2428)
                     /locus_tag="ACHOT6_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFP9949025.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTY
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     ISLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(2428..5781)
                     /gene="stfR"
                     /locus_tag="ACHOT6_003876"
     CDS             complement(2428..5781)
                     /gene="stfR"
                     /locus_tag="ACHOT6_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9949026.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGAMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAVAKKSASDASTSAREAATHATDAAGSARAASTSAG
                     QAASSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASAASTSAGQASVSATAAGKSAESAASSA
                     STATTKAGEATEQATAAARSASAAKTSETNAKASETRAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKSTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSETLAATPKAVKSAYDNAEKRLQKDQNGAD
                     IPDKGRFLNNINAVSKTDFADKRGMRYVRVNAPAGATSGKYYPVVVMRSAGSVSELAS
                     RVIITTATRTAGDPMNNCEFNGFVMPGGWTDRGRYAYGMFWQYQNNERAIHSIMMSNK
                     GDDLRSVFYVDGAAFPVFAFIEDGLSISAPGADLVVNDTTYKFGATNPATECIAADVI
                     LDFKSGRGFYESHSLIVNDNLSCKKLFATDEIVARGGNQIRMIGGEYGALWRNDGAKT
                     YLLLTNQGDVYGGWNTLRPFAIDNATGELVIGTKLSASLNGNALTATKLQTPRLVSGV
                     EFDGSKDITLTAAHVAAFARRATDTYADADGGVPWNAESGAYNVTRSGDSYILVNFYT
                     GVGSCRTLQMKAHYRNGGLFYRSSRDGYGFEEDWAEVYTSKNLPPESYPVGAPIPWPS
                     DTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRSWTIKGKPASGRAVLSQEQDGI
                     KSHTHSASASSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASA
                     VSQWSQNGSVHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGS
                     HGHTITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(5846..6445)
                     /locus_tag="ACHOT6_003877"
     CDS             complement(5846..6445)
                     /locus_tag="ACHOT6_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9949027.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDILGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSGDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIMGVGYKF"
     gene            complement(6512..9904)
                     /locus_tag="ACHOT6_003878"
     CDS             complement(6512..9904)
                     /locus_tag="ACHOT6_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949028.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQGYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNARGQRGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPDHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASRLEEFSKEWKDAN
                     DKWNAMWAVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRVSDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            complement(9964..10536)
                     /locus_tag="ACHOT6_003879"
     CDS             complement(9964..10536)
                     /locus_tag="ACHOT6_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9949029.1"
                     /translation="MKTGAEAIRALATQLPAFRQKLSDGWYQVRIAGQDVSTSGLTAQ
                     LHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGSFFTAGATLAAWGAAIGAGGM
                     TGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMVAQGNVLPVLY
                     GEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(10533..>10931)
                     /locus_tag="ACHOT6_003880"
     CDS             complement(10533..>10931)
                     /locus_tag="ACHOT6_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9949030.1"
                     /translation="HGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEA
                     TGLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQR
                     RTHSLWRHRAWRASAFTGIYNDLAAASTFV"
BASE COUNT         2501 a         3101 c         2602 g         2727 t
ORIGIN      
        1 tcaccagcca accgcagcac gttcttgcat acgacgtgtc tgcggttttt caaccattca
       61 gatacagcac tctcataaca ttcattcttc cgcattaaag tatatatgac catatcacct
      121 taatattttt cttcttcaga taaaaattgt tatctatgta tacttttaaa ccgaatccgt
      181 gtagagtctc tacctaagtt agttcgcagc tcccccttca gtttgcgcca ttaacctcct
      241 gacttttgcc gctatctgta agcattcctg tatacctgaa actacagggt tcatctacga
      301 actttaagaa cacccaggat aaaaattgtc aactatatta tatataacac catactaatt
      361 cgaggctata tgaacagcat actgataatc acttcactac ttatcatatt cagcattttt
      421 ggtcatgctc taataaaatt agggattggc atatccaata acccggacaa aacagatgta
      481 taagtcaaca tatcctgaat cagacataca atattgcaat gaaaaccaat aatattttaa
      541 ggaatatctt catgaaatca aaagacaccc taaagtggtt ccctgcgcag cttcctgaag
      601 tcagaattat cctgggagat gctgtcgtgg aagtagcaaa acagggaaga cctatcaata
      661 ccagaacatt gcttgattac attgaaggaa acataaagaa aaagtcatgg ctggataaca
      721 aagaattatt acaaacagcg atatcagttc ttaaagacaa ccaaaattta aatggtaaag
      781 tgtaataccc cccctgaaaa ataatttttc aatgcattaa aaaaatatca acaactacgt
      841 tattccgcaa caacctactt tttttaccat ttttccactt taacaacatt ttgctccact
      901 tttccacgac caaacaactt aaaatctggt tgaaataaca tacaacacta ttattcgtct
      961 tcctcgagtc cgcccggagt tcgaaaaaca aaccgagtta aagccatttt tcaaaaaatc
     1021 gatttttggt tacaccaaaa ttacgggact gcatccgaat tcgtttattt tcgaacgtat
     1081 acatacaaat atgtacaata ataagcgaaa taatttttgg atatgcatta tgatatgtac
     1141 accaatttcg tatagagtct cactatgtct cgaatttttg cttactgtcg gatatcaacg
     1201 ctggatcaga ccaccgaaaa tcaacgccgg gaaatcgaaa gtgcaggttt taaaatcaaa
     1261 cctcagcaaa taatcgaaga acacattagc ggctcagcag caaccagtga gcgtcctggt
     1321 tttaaccggt tgcttgctcg cctgaaatgt ggtgatcaat tgattgtgac aaaactggat
     1381 cgccttggtt gtaatgcaat ggatatcagg aaaacagtgg aacaactgac cgaaacaggt
     1441 atcagagtgc attgcttagc attggggggc attgacctga ccagtccaac aggaaaaatg
     1501 atgatgcaag taatttcagc agtcgctgaa tttgaacgag accttttact tgaacgcact
     1561 cattccggga tagtaagagc ccggggcgca gggaaacgtt ttggtcgacc acctgtgtta
     1621 aatgaagaac agaaacaggt ggtattcgaa cggattaagt caggtgtaag tataagtgcc
     1681 attgcccggg aattcaaaac ctcgcggcaa accattttaa gagccaaagc aaaacttcag
     1741 acacctgaca tataaaaaat aatcttggtg tgagatgctt tacgtcttcc aagccccctt
     1801 ccttgccgta aatggaaaga tacatctaat tatagaattt atatgtttta ccctacggca
     1861 gtgctggcca ttcaatatcc tgtgcagttg acgtatcaac acggttcagc aatacccgat
     1921 actttttcca tgcttccagc aacgagattt cttcctccgt tgcaatttcc agatctacag
     1981 catcctgaag cggcgcaata tgctcactgg ctacctgcat caggctgttt tttgtttctt
     2041 ccgcctcccg gatccggaac agtttttctg cttctgcatc tttcacccag gctgtgccgt
     2101 tccacttctg aaactcccct tccggcgata accaggtaac attttccggt aacggaccga
     2161 gttcagaaat aaataacgcg tcccctgacg ctacatcata aaccgtttta ccccgatggt
     2221 cttcaacgag atgccacgat gcctcatcac tgttgaaaac agccacaaag ccagccggaa
     2281 tatctggcgg tgcaatatag gtactgtttg ctggcagacc tgtatgaggc ggaatatatg
     2341 cgtcaccttc accaataaat tcattagttc cggccagcag attataaatt tttatggtcc
     2401 gtggttgttc actcattctg aatgccatta tgcaagcctc acaatatagt taaatgcgat
     2461 gtttttgacg gtgttttccg cgttaccagc agcgttaacg gtgatggtgt gtccatgtga
     2521 gccaatcgca acagagtgcg tatgagcacc aataccgaca gtatgtgcgt gagcacctgc
     2581 agatgcagct gtgccgctga cactatgagt gtgcgctcct gcagcacttg tattcacact
     2641 ggccgcagac actaccttat gtactgaccc attttgtgac cactggctga ctgctgatgc
     2701 gcctgtgaca ctatgagtat ggtttccggc acttgcggct gtaccgctca cactgtgcgt
     2761 atgcgccccg gtgttattcg tggatttagt gccgtaatca aacgacgatg tggttttcgt
     2821 ccccaaatcc gtactggatg cgctggcgct gtgggtgtgc gatttaatgc cgtcctgttc
     2881 ctgagacaat acggcccgac cactggcagg tttgccctta atcgtccagc tacgcatatc
     2941 agggatcacg cctgacggat aagcggctgc aagtttcggg tatgcagatt tgtcaaaagt
     3001 ctgcccctgc atcagggcat aaccagacgg aacggtatct gatggccacg ggattggtgc
     3061 gccgactggg tagctttctg gtggaagatt tttcgaggta taaacttctg cccagtcttc
     3121 ctcaaaacca tagccatctc ttgaggaacg gtagaacaga cctccatttc tgtaatgcgc
     3181 cttcatctgc agggtccggc aacttccgac tccggtatag aagttaacca gaatatagct
     3241 gtcgccagag cgggtgacat tgtaagcgcc tgattcggca ttccatggaa cgcccccatc
     3301 cgcatcggca tacgtatccg ttgctcttct ggcaaaagca gccacatgcg cggcggttaa
     3361 agtaatatct ttggaaccat caaactcaac accagaaacc agtcttggcg tttgcagctt
     3421 tgttgctgtt aatgcattac cgttcagact tgcggacagt ttggttccaa taaccagctc
     3481 gccggttgcg ttatcaatag caaacggtct taatgtattc cagccaccat aaacatcacc
     3541 ttgattggta agcagcaggt aagttttagc gccatcatta cgccataatg caccatactc
     3601 cccacctatc attcgaatct gattaccacc acgcgctaca atttcgtctg tggcaaaaag
     3661 ttttttgcac gacaagttat cgttaacgat taacgaatga gactcataaa aaccacgccc
     3721 actcttaaaa tcaaggataa cgtccgccgc gatacattca gtcgctggat ttgttgcccc
     3781 aaacttatag gtcgtatcat taacaacgag atcagcacca ggtgcggata ttgacaggcc
     3841 atcttcgata aacgcaaaaa cagggaaagc agcgccatca acatagaaca cagagcgcaa
     3901 atcatcgccc ttattactca tcattattga gtggatggct cgttcattgt tttgatattg
     3961 ccagaacatt ccataagcat aacgccccct gtcagtccag ccaccaggca taacaaatcc
     4021 gttaaactcg cagttattca tcggatcgcc tgcggttcgc gttgccgtgg tgataatgac
     4081 ccttgatgcc agttcgctta ctgagccagc agaacgcata acaacaacag ggtaatattt
     4141 tccagatgtt gcacctgcag gagcgttaac ccgcacataa cgcataccac gcttatcagc
     4201 aaagtctgtt ttactgaccg cgttaatgtt gttcaggaag cgtcccttat cgggtatatc
     4261 agcgccgttc tggtctttct gcagacgttt ctctgcattg tcataggctg attttactgc
     4321 ctttggcgtt gccgccagcg tttcagacgt actgttggtc gcactgctga gctgtactat
     4381 cccctttttc gtcgtgctcg catcctcaag cgccacggcg gatgcaatat cctctgcccg
     4441 ttttgccgct gtctcagcgc gcgttgctgc agattccgcc gtacttttgc tctgagctgc
     4501 tgccgtcgca ctaccagcag cctctgtcgc cttcgtggat gccgtcgtgg cgctgccctt
     4561 cgctgctgac gcctgtctgg tcgcctcatc ttttgaagca gacgccgatg aggccgatga
     4621 cgccgccgaa ctggcggacg atgccgctgc cgtttttgag gattctgcac gggtttccga
     4681 cgctttcgcg ttcgtttcgg atgtcttcgc tgcggaagct gacctcgctg ctgccgtggc
     4741 ctgctcagtg gcttcgccag ccttcgttgt ggctgttgaa gcggatgatg cggcgctttc
     4801 tgccgatttt ccggcggcgg tggcactgac tgaggcctgc ccggcacttg ttgacgcggc
     4861 actggcagac gacgcagccg ctgtttttga gcctgccgca gctgaggcgc tctgtcccgc
     4921 tgccgtctca gaagacctgg cgttcgtctc ggacgttttt gccgccttcg cggaatttcc
     4981 tgccgccgtt gccgaggaag cggcgctact ggcgctcgag gatgcgttcg tttctgatga
     5041 tttcgctgcc tcttttgagg ccgccgcatc ccgggctgag gtggcagctt ctgacgcttt
     5101 cgtggtcgcg gtggatgcag aagtggctgc tgattgttgt gacgctgcgg cattcgtttc
     5161 tgacgttttc gccgcaccgg cactggtggc cgccgcgctt tttgaggact ctgcagcggc
     5221 agcacttttt gaggcttcag tggcctttgt tgatgccgtt cctgcgctgg aagacgctga
     5281 ctgagccgac gaagcggcct gtccggctga cgtgctggct gcgcgtgctg agcctgcagc
     5341 atcagtcgca tgggttgccg cctcacgggc tgatgtgctg gcatcgctgg ctgacttctt
     5401 cgcgactgcc gtgttctgtg ccaccgcgga cgcgttacgc gccacctctt ccaccatcag
     5461 ttcaaaacgg cgcagtgcct caggacgggc atcatcctcc gtcatggcac cgagaaaatc
     5521 attcagcgta ccgggtcggg aatcttcata cacggtgatg gtcccggcat gtgacggcgg
     5581 gaatccttcc accaacagaa taacgctgta ctgaccgtac tcaacgtcca tgctgtaacg
     5641 cccggcttca tccggatttt ctgaggccag cgtgttcacc accaccgtgg tgctgttacg
     5701 ttttgctttc agctggattg tgcagttctg taccggtttt cctgtgccgt ctttcagtac
     5761 acctgaaatc tttactgcca tattcacccc acaaaaaagc ccgcctgaac aggcgggctg
     5821 tcataacact gtgttacctg gctaatcaga acttataacc gacacccatg atgaaaccgt
     5881 cagtgcgcca gtcgccactg ccggagcctt cataagcaat atcaatggcc acggattcgg
     5941 tcgggttaaa ctgcacgcca gctccccacg ccagagacgt gttgctgtgg cgaccgtcac
     6001 cacttccggt cagcacgtcg tgcgttttcc ccttgttgtc agttacgcgg agataatccc
     6061 cggagaaagt cgacacacgg ctgtaagcca cacccgccat cgcatacgcg ctgaaccatt
     6121 cattcacgcg cacagacggc cccgccatca cgctgaacca gcggttacga acggaatctt
     6181 catgccagcg ggtatcgctg taacgggtca gctggcgatt cttgtctcct gcatagctga
     6241 atgacgtcac cagccccagt atgtccgtaa actcataacg gtatttcacg ttaatcccgt
     6301 tcagatcatc gctgccggga acgttcgtcg aggcatgaag ataccccgcg ctcagcgtgg
     6361 actgatgttc agacgcccat gcaggcgcac cggatacggc cagacaaatg gctgcggaca
     6421 aaatggcggc ataaagttta cgcataatta cctctcgctt ttctgcaata aaaaaggcgt
     6481 catttctgac gcccgttatg ggttataaaa ttcagctgat actgatacct gctgtggatt
     6541 tcttcatcac cacaaccagc agatcgctga tactggttgt tggtgtccag ttattcgctc
     6601 ctgatgaaga tacggtgaat gtcagtgtcc cctgtccggc aggcatatct ataactgagg
     6661 aaaatacgcc ctgagcatcc gtcgtggact gattaaaaat ctcctgacca ttgcgggtca
     6721 ctcttaaccg gcaggttgaa taccagtatg actgttggtt attactgttg aaattctcat
     6781 gcttaccacc gcggaataac actggcggta tcatgacctg ccggtcaaac ttctgatcat
     6841 cactgactct taccgtgatg gtgccgctgg cataactgtt cgtgcggggg aaagacttgc
     6901 tgaccgtttt gacaatatcg ccttcaatct gattggctga cagtttcccc ttaatctgac
     6961 agttctcatt aatcgtgaca ttgttgagcg tcccggagtt cgcattcaca ctgccactga
     7021 tatccgcatt tttagcagtc agctttccgt ctgatgtcag ggaaaatacc ggaggactgc
     7081 caccactggt aatggttggg gccgtcaggc gcttcaggaa cacgtcgttc atgaatatct
     7141 gattgccctg cgccacaaac atcggcgttt cattcccgtt tgccgggtca ataaatgcga
     7201 tacggttagc ggcaaccaga aactgactca gtttgccttc ctccgtgtcc tccatgctga
     7261 ggccaatacc cgcgacataa tgtttgccgt ctttggtctg ctcaattttg acagcccaca
     7321 tggcattcca cttatcgttg gcgtccttcc actctttcga aaactcctcc agtctgctgg
     7381 cgttatcctc cgtcagctcg actttttcca gcagctcctt gccgagatgg gattcggtta
     7441 tctgaccttt gaaaaaatcc agatagcctt ccgcatcatc gctcgcccga ccaacagcct
     7501 ctacgaatgc cgatttgcca acggtgttca cactgcggat ataaaagtaa taatcatggt
     7561 ccggtttgat attgatactg gcagctatcc agtacagcgc cgtgccaaga tagcgggctg
     7621 tggtttcaac ctgcctgata tcagcaatcc gtttttccga gaaccagaac tcaaactgta
     7681 ccgtcgggtc ataaaccgca agatgcggcg tggcggttat ctgaaaatag cccggtgtca
     7741 gctcaatccg cgacggcgct gccggggcgg caatccggaa cgataccgac gccggatcgc
     7801 cccgctgccc ccgggcattt accgcccgga ctgtcagcct gtagttcccc agcgccagtt
     7861 gtgtgaagcg gtaagtggtt tccgtcgtcc gggccgtgct gaccagccgc tcactgccgt
     7921 catccgctgc cacggtcagg cgaagcagga agctcacgcc cttcaccacc ttcggcgtgt
     7981 cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggcg gtcaggtgct
     8041 gcaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca aagtgcgccc
     8101 cgttatccac gatggcctct ttttccggta catgctgcac ggcggtgatg gcatacgtgc
     8161 cgtcgtcatt ctcacggata ctcacgcagc ggaacagtcg ctggcgcagc gtcggcagct
     8221 tcagtcccca cacgctgtat tcggcaacgc cgtcaggaac ccggctcact ttcaccttca
     8281 cgccgtcggt gacggactga acctccacgc tgaccggatt gccacttccg tcaaccaggc
     8341 ttatcagcgt ggtaccggag gatggcagcg tgatttcacg gtcgagcgtc agcgtccggg
     8401 tctggctgtt caccgccagc acgcgcccgc ctgtgcggat accggcatag tcatcatcgc
     8461 agatttcaat gacatcgccc ggtacatggc gaagcccttc ggcacccacg ctgaagtcca
     8521 cggtctgcgt ttccagcagt tctgttttaa tcagccacag cccggcgcgg tgcgcctgcc
     8581 cccggctggt acagccaaag gcatccatct tcgtgacatt acgaccgtaa cgggcaatgg
     8641 cctgcgtgtc ctccacaagc tctgttgccg tttcccagcc gttatccggg tcaatccagt
     8701 tcacctcaac ggcattatgg cggtccttca gggcgctgaa actgtagcgg aacggcgcgc
     8761 catcatccgg catcaccaca ttactgcggt tataggtcca caccttatcc gacggccggt
     8821 cctgcacgaa cgtcagcgtc tgcccgttcc ataccggcat acagcgcatc gccgagcaga
     8881 aatcactgag cacatcccac gccttgcgct gtgtggtcag gtacgcatta caggtgatgc
     8941 gcggctccgt gccgccaaag ccgtccggca ctgactggtc gcagtactgg ccgatgacat
     9001 acagcgccca tttatccaca tccgccgcac caagacgttt ccccatgccg tagcgcggat
     9061 gggtcagcat atcccacaga caccaggcca tgttgttgct gtatgccggt ttaaacgttc
     9121 cgtcccagat accgctgtat tgccgcgtct gcgggttata gttcgacggc acctgcagaa
     9181 tgcgcccgcg aagatgataa ttacggctca cctgctggct gccgaactgc tccgaatcca
     9241 cctgcacgcc gaccagtgcc gtgttcgggt agccctgttt cacatcgatg atttcggtgt
     9301 atgacgacca gagcgttttg ttctgcagct ggtctgtggt gctgtccggc gtcatcctgc
     9361 gcatccggat attgaacggg cgcggcggca ggttacccac caccaccgag gccagatact
     9421 gcgaggtggt tttgccctta atggtgatgt ctttttccgt cacccagcca ccattacgct
     9481 ggatctgaac cagcaggcgg acttccgacg gattcctgtc cccctttgag gtggtttcca
     9541 ccagtgcctg cacgccgaag gtaaaacgca gtcggtcaat gtttgccgac gtgatggtcc
     9601 gggtgatcgg cgtgtcgtat ttcacttccg tacccagcac cgtctcggag ccggaggatt
     9661 caaatccctc cggcggtgtc tgctcctgct caccggcccg gaacaccacc gtgacgccgg
     9721 agatattggt attcccctca ctatccagca ctggcgtact gttcagcagc acgcttttta
     9781 atccatccac cggaccttca accggccctt cgctgatggc atcgatcaca ctcagcaact
     9841 gcgtggactt caggttgtcc ttcgcttcgc gcggagtatg ccccttactg ctgcctttac
     9901 ccattcctca cgctccataa acgacaaaac cgcccggagg cggtttcaca taaacgtttt
     9961 tcatcagcga ccaatcacca caacctgacc accatcccct tcgtctgccg tgctgatctc
    10021 ctgagagacc acccgcgacc ccacgcgcat ttcaccgtac agaaccggca gaacattgcc
    10081 ctgggcaacc atgttatcca gtgaggagaa ataggtgttc tgtttgccgt tatccgttgt
    10141 ctgtgtacgg ggagttctgg ctttcggtgc cagcatctgc gccacaccac cgagcaccat
    10201 actggcaccg agagaaaaca ggatgccggt cataccaccg gccccaatgg ctgcccccca
    10261 tgctgcaagg gtggccccgg cggtaaagaa tgatccggca atggcggcag cccccaggac
    10321 aatctggaat acgccacctg acttggcccc ggcgactctg ggaacaatat gaatcacagc
    10381 gccatcaggc agagtctcat gtaactgcgc cgttaatccg gacgtgctga catcctgccc
    10441 ggcaatccgt acctgatacc agccgtcgct cagtttctga cgaaacgccg ggagctgtgt
    10501 ggccagcgcc cgaatggctt cagcccccgt tttcacacga aggtcgatgc ggcggccaaa
    10561 tcgttgtaaa tccccgtaaa ggcagatgcg cgccatgccc ggtgacgcca gagggagtgt
    10621 gtgcgtcgct gccatttgtc ggtatacctc tctcgtttgc tcagttgttc aggaatatgg
    10681 tgcagcagct cgccatcacc acagtaaatg gcggcatgat tcggcaccga tgaaccaaaa
    10741 cagcacagca gcacatcgcc cggttgtgct gatgacaacg gcacctgata cagccctgtg
    10801 gcctccagat tatccagata gagattctga ccgtgacgcc accagtcatc ctcgcgatga
    10861 aaatccggca tctcaatccc cgccagatga taagcatccc ggaacagcgt gtaacagtcc
    10921 gtcaccccgt g
//
LOCUS       DAZKIV010000152        10903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZKIV010000152 DAZKIV010000000
VERSION     DAZKIV010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.152"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHOT6_003881"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHOT6_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFP9949031.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHOT6_003882"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHOT6_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFP9949032.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHOT6_003883"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHOT6_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFP9949033.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGNGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYFDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHOT6_003884"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHOT6_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFP9949034.1"
                     /translation="MKILIIEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHOT6_003885"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHOT6_003885"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFP9949035.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHOT6_003886"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHOT6_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFP9949036.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHOT6_003887"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHOT6_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFP9949037.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHOT6_003888"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHOT6_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFP9949038.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTIPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMLEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHOT6_003889"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHOT6_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFP9949039.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVRDAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETGDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2723 a         2851 c         2689 g         2640 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagct gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc tactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcac tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga cccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggcagttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccaattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatctt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggcagtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggagc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actagcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt aatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acttcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattattg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaaccgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgctgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt gtttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtacgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcaccgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttgaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacggcttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatggcgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacagtg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caagttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcaac atgctgctgg aacgctgggg tctcttccag ggcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccaccacgcc cgcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggtgagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gtccgccaag cggtcgaatc
     7261 agaaagggaa cggagaaagt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcgaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aattggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgagcatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccaa cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaatggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg tgccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg gcaggcatcg ccaaatgccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgatc acctgcctgc accagtaggc
     8221 ctcgttgcgt taacgccttt tccttccagc ggcaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctg gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccattgtc agtcgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcacg cgcgcaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aatgctcgtt gcaacacact
     8941 gtaatcaccc gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcatc
     9061 ctggcgcgaa atgacttcac tgaacagctg gcgagtgttg acgtggggaa ctactccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacgc atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc
     9361 attcagcgca tcttgttgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg
     9601 caaagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc gctggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcatcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactc tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttcc ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcagaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaattg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcgtcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttgttact tttatttagc cag
//
LOCUS       DAZKIV010000153        10673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZKIV010000153 DAZKIV010000000
VERSION     DAZKIV010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.153"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..366
                     /locus_tag="ACHOT6_003890"
     CDS             70..366
                     /locus_tag="ACHOT6_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFP9949040.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRHGASKKAITRAFDDDVEFQERMAEHIQYMVETIAHHQVDIDSEV"
     gene            372..1157
                     /gene="bet"
                     /locus_tag="ACHOT6_003891"
     CDS             372..1157
                     /gene="bet"
                     /locus_tag="ACHOT6_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFP9949041.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQF
                     IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNE
                     SCTCRIYRKDRNHPICVTEWMDECRREPFKTREDREITGPWQSHPKRMLRHKAMIQCA
                     RLAFGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDD
                     DLLPLCSQIFRRDIRASSELTQAEAVKALGFLKQKATEQKVAA"
     gene            1154..1834
                     /locus_tag="ACHOT6_003892"
     CDS             1154..1834
                     /locus_tag="ACHOT6_003892"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFP9949042.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERNEKYMANFDEMVPEFIEKMDVALAEIG
                     FVFGEQWR"
     gene            1831..1992
                     /locus_tag="ACHOT6_003893"
     CDS             1831..1992
                     /locus_tag="ACHOT6_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 family protein"
                     /protein_id="HFP9949043.1"
                     /translation="MKHPHDNIRVGAITFIYSVTKRGWVFPGLSVIRNPLKAQRLAEA
                     INNKRGLYD"
     gene            1985..2542
                     /locus_tag="ACHOT6_003894"
     CDS             1985..2542
                     /locus_tag="ACHOT6_003894"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000129280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9949044.1"
                     /translation="MTDFTGSNTPAEHRDSWRTPPEIFAALNAEFVFQLDAAASEKNR
                     LCRLFISQEQNTLTTSWPEAMGYASGYVWLNPPYSNISPFVKKAATENKFSSVGCVML
                     LPADTSVGWFHEAIQTASEVRFITAGRLAFINPLTEKPVSGNNKGSMLIIWHPYPRTH
                     CHFTTVDRGELMAFGSRILARREAA"
     gene            2619..2834
                     /locus_tag="ACHOT6_003895"
     CDS             2619..2834
                     /locus_tag="ACHOT6_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFP9949045.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            2933..3151
                     /locus_tag="ACHOT6_003896"
     CDS             2933..3151
                     /locus_tag="ACHOT6_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9949046.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRQNYQTVSATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEEIELKNKQWGL"
     gene            3199..3477
                     /locus_tag="ACHOT6_003897"
     CDS             3199..3477
                     /locus_tag="ACHOT6_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9949047.1"
                     /translation="MFRIIFPNTWYVDHHGTPCKILRSTHNKVHYIRKGRTCIASMFR
                     FNHDFEPVNKADADRIAEEIETAEHIKKLRDMRSKSRGNHGIIQPHTR"
     gene            3449..3514
                     /locus_tag="ACHOT6_003898"
                     /pseudo
     CDS             3449..3514
                     /locus_tag="ACHOT6_003898"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3676..4839
                     /gene="intD"
                     /locus_tag="ACHOT6_003899"
     CDS             3676..4839
                     /gene="intD"
                     /locus_tag="ACHOT6_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9949048.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKELPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTARKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHSGIMEDIKKA"
     gene            complement(4855..4931)
                     /locus_tag="ACHOT6_003900"
     tRNA            complement(4855..4931)
                     /locus_tag="ACHOT6_003900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4895..4897),aa:Arg,seq:tct)
     gene            5174..5806
                     /gene="fimZ"
                     /locus_tag="ACHOT6_003901"
     CDS             5174..5806
                     /gene="fimZ"
                     /locus_tag="ACHOT6_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFP9949049.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRTVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKNNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(5809..6324)
                     /gene="sfmF"
                     /locus_tag="ACHOT6_003902"
     CDS             complement(5809..6324)
                     /gene="sfmF"
                     /locus_tag="ACHOT6_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFP9949050.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(6335..7342)
                     /gene="fimH"
                     /locus_tag="ACHOT6_003903"
     CDS             complement(6335..7342)
                     /gene="fimH"
                     /locus_tag="ACHOT6_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFP9949051.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(7355..9964)
                     /gene="sfmD"
                     /locus_tag="ACHOT6_003904"
     CDS             complement(7355..9964)
                     /gene="sfmD"
                     /locus_tag="ACHOT6_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFP9949052.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPARVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     IIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQATYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(9995..>10673)
                     /gene="fimC"
                     /locus_tag="ACHOT6_003905"
     CDS             complement(9995..>10673)
                     /gene="fimC"
                     /locus_tag="ACHOT6_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFP9949053.1"
                     /translation="KLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTAVWIRN
                     SHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAADRESLF
                     WMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSRSGNYI
                     NVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDYGSVTP
                     VREVNLN"
BASE COUNT         3021 a         2549 c         2489 g         2614 t
ORIGIN      
        1 tttcctgttt tcctaatcag cctggcattt cgcgggcgat attttcacag ctatttcagg
       61 agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag ctgggcgcgt
      121 cactaccagc agatcgcccg tgaagagaaa gaggcagaac tggcagacga catggaaaaa
      181 ggcctgcccc agcacctgtt tgaatcgcta tgcatcgatc atttgcaacg ccacggggcc
      241 agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga gcgcatggca
      301 gaacacatcc agtacatggt tgaaaccatt gctcaccacc aggttgatat tgattcagag
      361 gtataaaacg gatgagtaca gcactcgcaa cgctggcagg gaagctggct gaacgtgtcg
      421 gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg gcatttaaag
      481 gtgatgccag cgatgcgcag ttcatcgcat tgttgatcgt cgccaaccag tacggcctta
      541 atccgtggac gaaagaaatt tacgccttcc ctgataagca gaacggcatc gttccggtgg
      601 tgggcgttga tggctggtcc cgcatcatca atgaaaacca gcagtttgat ggcatggact
      661 ttgagcagga caatgaatcc tgtacatgcc ggatttaccg caaggaccgt aatcatccga
      721 tctgcgttac cgaatggatg gatgaatgcc gccgcgaacc attcaaaact cgcgaagaca
      781 gagaaatcac ggggccgtgg cagtcgcatc ccaaacggat gttacggcat aaagccatga
      841 ttcagtgtgc ccgtctggcc ttcggatttg ctggtatcta tgacaaggat gaagccgagc
      901 gcattgtcga aaatactgca tacactgcag aacgtcagcc agaacgcgac atcactccgg
      961 ttaacgatga aaccatgcag gagattaaca ctctgctgat cgccctggat aaaacatggg
     1021 atgacgactt attgccgctc tgttcccaga tatttcgccg cgacattcgc gcatcgtcag
     1081 aactgacaca ggccgaagca gtgaaagctc ttggattcct gaaacagaaa gccactgagc
     1141 agaaggtggc agcatgacac cggacattat cctgcagcgt accgggatcg acgtgagagc
     1201 tgtcgaacag ggggatgatg catggcacaa attacggctc ggcgtcatca ccgcttcaga
     1261 agttcacaac gtgatagcaa agccccgctc aggaaagaag tggcctgaca tgaaaatgtc
     1321 ctacttccac accctgctgg ctgaggtttg caccggtgtg gctccggaag ttaatgctaa
     1381 ggcgctggcc tggggaaaac agtacgagaa cgacgccaga accctgtttg aattcacttc
     1441 cggcgtgaat gttactgaat ccccgatcat ctatcgcgac gaaagtatgc gcaccgcctg
     1501 ctctcccgat ggtttatgca gtgacggcaa cggccttgaa ctgaaatgcc cgtttacctc
     1561 ccgggatttc atgaagttcc ggctcggtgg tttcgaggcc ataaaatcgg cttacatggc
     1621 ccaggtgcag tacagcatgt gggtgacgcg aaaagatgcc tggtactttg ccaactatga
     1681 cccgcgtatg aagcgtgaag gcctgcatta tgtcgtgatt gagcggaatg aaaagtacat
     1741 ggcgaatttt gacgagatgg tgccggagtt catcgaaaaa atggacgtgg cactggctga
     1801 aattggtttt gtatttgggg agcaatggcg atgaagcatc ctcacgataa tatccgggta
     1861 ggcgcgatca ctttcatcta ctccgttaca aagcgaggct gggtatttcc cggcctttct
     1921 gttatcagaa atccactgaa agcacagcgg ctggctgagg cgataaataa taaacggggg
     1981 ctgtatgact gatttcaccg gaagcaatac tcctgccgaa catcgcgaca gctggcgcac
     2041 accaccagag atttttgctg cgcttaatgc agagttcgtt tttcaacttg atgctgccgc
     2101 cagcgaaaaa aaccgactat gtcggctttt tatctcacag gagcagaaca cattaaccac
     2161 ttcatggcct gaagcaatgg gatatgcctc tggttatgtc tggttgaatc caccatacag
     2221 caatatttcc ccttttgtga aaaaggcagc cactgaaaac aaattcagta gtgtgggatg
     2281 tgtaatgtta ttgcctgctg acacatctgt cggatggttt catgaagcga tacaaaccgc
     2341 cagtgaggtc agattcatca cggcaggacg actggcattt attaacccac tcactgagaa
     2401 acccgtcagt ggaaataata aaggctcgat gctcattatc tggcacccat acccccgtac
     2461 acactgccac tttacgaccg ttgatcgtgg agagttgatg gcgttcggct caaggattct
     2521 tgcccgtcgg gaggctgcat gacaaccacg gaatgcattt ttctggcagc gggcttcata
     2581 ttctgtgtgc ttatgcttgc cgacatggga cttgttcaat gacacctcag caggaaaacg
     2641 cccttcgcag cattgcccgt caggctaatt ctgaaatcaa aaaagccaga cagcagtttc
     2701 cggataaaaa cgtcgatgac atttgccgta gcgtacttaa gaagcaccgc gaaacggtaa
     2761 cgctgatggg attcacaccg actcatttaa gcctggcgat cggcatgtta aacggcgtct
     2821 ttaaggaacg gtgaacatga aaagcaaaat catcagggag ctacaggctc cttttttatt
     2881 gttcgcattc atcctcaagc gtattaacca acaattcagg gattaatgga agatggcaga
     2941 catcattgat tcagcatcag aaatcgaaga attacagcgc aatacagcaa taaaaatgcg
     3001 tcgtcagaac taccagactg tatccgcaac tcattgttgt gagtgtggcg atccgataga
     3061 tgagcgaaga cgcctggctg ttcagggttg tcggacttgt gcaagttgcc aggaggagat
     3121 cgaacttaag aacaaacaat ggggactgtg atggcctcaa agcagcaaat ttcaacatcg
     3181 tccaactgag gtgtaaaaat gttcagaatc atttttccta acacctggta cgtcgaccac
     3241 cacggcactc cctgcaaaat cctgcgttct acccacaaca aagttcacta catccgaaaa
     3301 ggcagaacat gtatcgccag catgttccgc tttaatcatg actttgaacc tgtgaataaa
     3361 gctgatgcag atcggatagc agaagagatc gaaacggcag aacacattaa gaagttacgt
     3421 gacatgcgtt caaaaagcag aggtaaccat ggaatcatac agcctcacac tcgatgaggc
     3481 ctgtcagttt cttaagatat ccagaccaac cgccaccaac tggatacgaa caggccgcct
     3541 acaggcaaca cgtaaagatc caaccaagcc aaaatctcct tacctcacaa cacggcaagc
     3601 ctgcattgcg gcgcttcagt ctccgctgca tactgtccag gtgagcgcgg gtgatggcat
     3661 aacagaggaa agaaaatgtc actcttccgc agaaatgaaa tatggtatgc ctcgtattcg
     3721 ctcccgggcg ggaaacgaat taaggaatct cttggcacaa aggacaagcg gcaagctcag
     3781 gagttgcacg acaagcgaaa agcagaactc tggcgagtag aaaagctagg ggatttacct
     3841 gatgtcactt ttgaagaggc ctgcctaaga tggcttgagg aaaaagctga taaaaaatct
     3901 ctcgattcag ataaaagccg gattgagttc tggcttgaac attttgaggg tataaggctt
     3961 aaagatatct cggaggcaaa gatttactct gctgtaagca gaatgcataa cagaaagacg
     4021 aaagaaatat ggaaacagaa agttcaggcc gccatcagga aaggtaaaga actgcctgtt
     4081 tatgaaccaa agccagtatc aactcagaca aaggcaaagc atcttgccat gataaaggcc
     4141 attctccgtg ctgcagaacg cgactggaag tggctggaaa aagcgcctgt catcaagata
     4201 ccagcggtca gaaacaagcg agtcagatgg ctggaaaagg aggaagcaaa acgccttatt
     4261 gatgagtgcc ccgaaccact gaaatctgtc gtcaagtttg cgctggcaac tggtctgaga
     4321 aagtcgaaca tcataaatct ggaatggcaa caaatcgaca tgcagcgacg agttgcctgg
     4381 gtgaatccag aagagagcaa atcaaaccgc gccattggtg tggcgctgaa cgataccgcc
     4441 cgtaaagtgt tgcgtgatca aataggcaag catcacaaat gggtgtttgt acataccaag
     4501 gcggctaagc gagcagatgg aacatcaacg cctgcggtca ggaagatgcg catcgacagc
     4561 aagacatcat ggctatcagc ttgtcgtcgt gcaggaattg aagatttccg tttccatgac
     4621 ctcagacaca cctgggcaag ctggctgatt cagtcaggcg tcccattatc agtgcttcag
     4681 gaaatgggcg gatgggagtc catagaaatg gttcgtaggt atgctcacct tgcgcctaat
     4741 catttgacag agcatgcgag gaaaatagac gacatttttg gtgataatgt cccaaatatg
     4801 tcccactctg gaattatgga ggatataaag aaggcgtaac tgattgaatt gtaatggcgc
     4861 gccctgcagg attcgaacct gcggcccacg acttagaagg tcgttgctct atccaactga
     4921 gctaagggcg cgttgatacc gcaatgcggt gtaatcgcgt gaattatacg gtcaaccctt
     4981 gctgagtcaa tggcttttga tctggttgct gaacaagtga acgaccgcgt ctgattttct
     5041 gatttatttc gctatagcgg caaacaaacg cacaccgctg cgcgtctgaa tcaagaaaac
     5101 ccgtattttc atgtatcaaa gtacaattta ccgccctaac ggaaaattgt ccgctcctat
     5161 gagactggta actatgaaac caacgtcggt gatcattatg gatactcatc ctatcatcag
     5221 aatgtctatt gaagttctgt tgcaaaaaaa cagtgaattg cagattgtcc tgaaaacgga
     5281 tgattaccgc ataaccatcg attatctccg aacccgtact gttgatttaa tcattatgga
     5341 tatagacttg cccggaacag acggttttac cttcctgaaa aggatcaaac aaatccagag
     5401 cacagtgaaa gtgttatttt tatcatcgaa atcagaatgc ttttatgctg gcagagcgat
     5461 acaagctggt gctaacggtt ttgtcagtaa atgcaatgat cagaatgata tttttcatgc
     5521 cgttcagatg atcctctccg gatacacgtt ttttcccagc gaaacgctta actatataaa
     5581 aaacaataaa tgtagtacga atagttcaac ggtcactgtg ctatctaatc gtgaagtgac
     5641 catattacgt tatctggtta gcggattatc taataaagaa attgccgata agttattact
     5701 tagcaataaa acagttagtg cgcataaatc taatatttat ggcaagctag gtttgcattc
     5761 aattgtagag cttatcgact acgccaaatt atacgaatta atataatatt aattataatt
     5821 gatcataaat atcgcatccg ctttcgccac acctggccga acaccgctgg ctaacgctcg
     5881 ataattggca aaaaagttta gtgtgacatt gccatttgca tctacttcct cagtcgggct
     5941 cgccgctccc agtgcgagcc gggagcgatc gctattacgt aattcgatgg cgatggtttg
     6001 cgccattgcg ggatcatcca gagccagcag gttggtatcg gatgccggcg ttcccgtaaa
     6061 taaaatcgca actgaacccg gaggacatcc ctccagccgc aggctaaaag ggacgagtgc
     6121 cgtggtatcg ccagcgttca gtaattgtgt cgtaggccat ctgcctaaat ctaccgtctt
     6181 atcaatatcc gcagtgttta cggtacagga gaaatcaaca acgttaccgt gcaaattgat
     6241 attaatcgtt cccaaagggt cagctgccca tccactggaa ctccacagta acccgcagaa
     6301 acagctaaag agtactcttc tcattctccg tacctcattg ataatcgacg cgtaaatacc
     6361 ccagcgcgct aaacggccct tcggtcggtt tttgaccggt aatactgata ggccaggcgc
     6421 gaagtgtgac attggctgcc gcagctgcat ccagacggaa aggaataacg ctattgagat
     6481 cgttaggcgt gatcggcgta tcgttctgat cggcgacaat aaaacctaaa tcctgattgt
     6541 ccgacaccat cgcctgacca gaaacggcac tggcttccag acgcattgtt aaataagcct
     6601 gcgcagcaac attcgtacat ttgaccgcaa tgctcttggt ttgcggcatg acaccagcag
     6661 gtcgattacc cggccctgcc gcactaaata acgatgcgcc gatatcacca aaatcaaatt
     6721 caacaatctg cccggcattt aattcgcagt tttgcggtac ttcaacccgg ccaccaaaac
     6781 taatggtata aacaggtgta accaacggat cgtaaggcgt tgatgtcaca tacacggtaa
     6841 acatcacctg tctggggatc tccaccatgt taataaaggg acgaataacc tttaaacgaa
     6901 atattaaacg agaatccatc accccgaatg gaaggttttg tgaaacgtta ggatctgttc
     6961 ccatccgaat ataattttgc ggggggaact gaatatccat cacactatca accagactca
     7021 tcgcacctaa tagataatca tttaattgca tatatttata attatcgata gtttcctgaa
     7081 caataaagtt ggtgacataa ctacggtatg tataattcac cagcgtgccg ggtggacaaa
     7141 ttgctgacac acctacccag ccggattttt ccggtaaaac aacaatctgc cctggctggt
     7201 tattggtgct attaaagata tttgagagat catagtgttc atcttctgct acgccagtac
     7261 tatttgcaca aacagtagcc caggatatgt ttgccagaca caaacatgcc atcgccaata
     7321 ataatctgca gattatcttc attgataatt tcctttatga gccaggatgt gtgcaaactg
     7381 ccgaaataac agtgacggct tgctgtaagc tttgcttcgg caatacatag tgcgcaatgc
     7441 agcgtgagtt cgcgccgtct ccccactgaa ccagtaattc accagacaat ggcgcaccac
     7501 ttaaataaac ttgcccgtca tcacccacca tactggttat tccggtactg ttttcccgta
     7561 ccagtgatcc aaacggcacc ggttttccgc cctgggtaac ggtaatgagc gcccgcacgc
     7621 ctatgcgggt atcaaaattg gcacgaacca acgcgccttg cgtcggcaca acgctgctaa
     7681 tatttttttc aacatcgatg gaattcccca tcgtattggt atcaagcgcg atacggttat
     7741 accgataaac cgtggcatac ggcatcaccg catagccgcg ccagtcggtt aaaatgccag
     7801 tttgattttc aatgcgtaca ccgcctgcgc caggcgcttt aatcaaaaca ttggtatccc
     7861 ctaaaggctg gctcagcgtt atgccatttt cgtgcccgac cacaccgcca gacagctgcc
     7921 agttaacgtc atgttgatcg cggtcgtagt tatacccgac ccccagcgta ccgtaagtgg
     7981 cctgccagtt tgccgtggcg ctgcccgtat acccattatt cgaggtatca ccctggctta
     8041 cgtgataact caggttgtgg ccttccagta aggtcccacc tacaccagcc agccagctat
     8101 tttgcccgtt gctgttacgg ttggcgttaa aggaggcata agcgcggtcg agcgcatttt
     8161 cccgggtgta gcgacgtttg gtcagaacat tgaaaggcac tgaaacatta agtccgacaa
     8221 tacgttcgtt atcggggatc cctacagatt cattccacga aaatgagagt gaataactga
     8281 tgccaaccca gctgctggta taccccacct gataccaggt atctgaatcc gaagtattcc
     8341 agtatttttg atgggtacca gagatataaa gcgagccaaa gtcattaagt gattgtgaaa
     8401 tatttaactg caaacggtct ttacggctaa agcgtaaatt gtggtaattc acgattatca
     8461 gttcatcccg atgctcaccg tcgtaatcgt aatcatattc gtacccctcc attcgacgat
     8521 acgcaacatc atcaagggta taaaaacctt gtgtcgaata gcggtaaccc attaactgaa
     8581 aattggtgcc gaaggtgttc atcgatttcg catagaggaa gcgaatggaa tccccctcat
     8641 gacgactgtc gtcggctaac tgactgcgcg catgggtcac atccagcgac actgcccccc
     8701 agtttccgag attgcgcccc agtcctaata aaaaggcggt gtaattggca gataattgcg
     8761 tcccgccgta ggcagtaaat tcctgtggta aaccgccgag tgccgtgccc tgaaaaaaga
     8821 aaggtgatga ttgctgacta ttaccgctac gaaaatcgcc cgccgtcagg tcaaatttga
     8881 aacgcccttc gcgttgtaaa atcggcactg ttgaatacgg aattgtgtaa ttctgctgat
     8941 tgccatcgcg ctcgtcgatg atgacatcca gatcgccatt tgaagatgtc gggtgcaaat
     9001 ctgtaatttc aaaagcgccg ggggaaacat agctttgata gataataaaa ccattttgcc
     9061 gaatcgttag ctgggccgcc gtacgggcaa tcccacgtac cgttggggca aacccttgct
     9121 ggctatcagg atacatatta tcggaagaat aaagccgtac accacgaaat ccaacaccat
     9181 cgaaaatatc acttcctgta ttgccgtctc ccattaccag ttcacttttc agcggaataa
     9241 tggcgcgctg tacccaggtg ccaatattat tccactgttc tgaatgatat ccatttccgc
     9301 gaaaatagtt ccaggaacca ttgttgcgta aacgccacgg gccaatatta atcccgctga
     9361 gctcactaaa aaaatagcta tcgttaccat tacctctgtt accggtgaaa ttataattca
     9421 ggagtaaagc aggaatacct tcatcccact cttcaggcgg aatgtaaccg tgagcgctac
     9481 tcaacaacgt tatctgagga atagtgatgt tcaggcgcat cgctgcaaaa tcaaagttaa
     9541 ttgtggcatc aggtacagct ttcagtaaat tgatgcattt attgttttgc tgctgggcta
     9601 actcgggaaa tgcactgcta ttaaggccaa ttcgttcaag gagtacctgg ttaaaacagg
     9661 gcattaaccc acctgattta tcacctgtat tttctgttgt cgattcaaat acgatatcct
     9721 gtgaaccaat gaactcatca ttacgccaga gatccacccg atacacgcgc gcaggttgat
     9781 gattacctct ctcaaagcgc gataaatcag caacggatgt gccattctct aataaaaatg
     9841 ccgggttgaa ataactttcc gcatagctta aaaaggaggg taaaatggca agagatgaat
     9901 aacaaattcc ggccagacac caggtatacc tctgcggaat atccgtagta gtgggtattt
     9961 tcataattag ctctgatatt gtcagatgat tcggttagtt taagttcact tctctgaccg
    10021 gggttactga accataatca ttaacggtct gaaatttcag ctttccctgc actcctgagg
    10081 gtaagggaat ttgtgaacta actctgggtg ccgccatagc attccccaac ttttggctgc
    10141 ccacttgcag gttaacgagg gtgacataaa aaggtgatgg attatgaaca ttgatatagt
    10201 taccggatcg cgaaaacttg agtgtgtccg gcgcttctgc gggtaattct tgtaattgaa
    10261 ttggacggag aaataatttc atgcgcgata aaatcgccag ttgcaaaaca ttcctgccgt
    10321 tcaatgcatt tttatctacc gaagggatcg tcttaacatt catccagaac agagactcac
    10381 gatctgccgc cagcggtgga ccggtgtaaa taatacgcaa ggtattttcg cttttgggtt
    10441 cactaacaaa cagtggcggt gtaatgatga atgacttttc ttttacaccg ctgctgtttt
    10501 caatccacga attgaccaga aagcgctcat tggtatggct atttctaatc cataccgcag
    10561 tctgtttagc atcagcggga taaataatac gcgtggcacc taatgcgatc cctccggcag
    10621 catgggcgct ggccgaaatg attaaataaa atataatgag catcaataac ttt
//
LOCUS       DAZKIV010000154        10654 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZKIV010000154 DAZKIV010000000
VERSION     DAZKIV010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10654
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.154"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            125..1492
                     /gene="dcuC"
                     /locus_tag="ACHOT6_003906"
     CDS             125..1492
                     /gene="dcuC"
                     /locus_tag="ACHOT6_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9949054.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSNGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKALDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1535..2032)
                     /gene="sspB"
                     /locus_tag="ACHOT6_003907"
     CDS             complement(1535..2032)
                     /gene="sspB"
                     /locus_tag="ACHOT6_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFP9949055.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2038..2676)
                     /gene="sspA"
                     /locus_tag="ACHOT6_003908"
     CDS             complement(2038..2676)
                     /gene="sspA"
                     /locus_tag="ACHOT6_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFP9949056.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3071..3463)
                     /gene="rpsI"
                     /locus_tag="ACHOT6_003909"
     CDS             complement(3071..3463)
                     /gene="rpsI"
                     /locus_tag="ACHOT6_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFP9949057.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3479..3907)
                     /gene="rplM"
                     /locus_tag="ACHOT6_003910"
     CDS             complement(3479..3907)
                     /gene="rplM"
                     /locus_tag="ACHOT6_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFP9949058.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4126..5253)
                     /gene="zapE"
                     /locus_tag="ACHOT6_003911"
     CDS             complement(4126..5253)
                     /gene="zapE"
                     /locus_tag="ACHOT6_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFP9949059.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5447..5845
                     /gene="zapG"
                     /locus_tag="ACHOT6_003912"
     CDS             5447..5845
                     /gene="zapG"
                     /locus_tag="ACHOT6_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFP9949060.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            5999..7366
                     /gene="degQ"
                     /locus_tag="ACHOT6_003913"
     CDS             5999..7366
                     /gene="degQ"
                     /locus_tag="ACHOT6_003913"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFP9949061.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKATEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7456..8523
                     /gene="degS"
                     /locus_tag="ACHOT6_003914"
     CDS             7456..8523
                     /gene="degS"
                     /locus_tag="ACHOT6_003914"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFP9949062.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8586..9524)
                     /gene="mdh"
                     /locus_tag="ACHOT6_003915"
     CDS             complement(8586..9524)
                     /gene="mdh"
                     /locus_tag="ACHOT6_003915"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFP9949063.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            9959..10429
                     /gene="argR"
                     /locus_tag="ACHOT6_003916"
     CDS             9959..10429
                     /gene="argR"
                     /locus_tag="ACHOT6_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFP9949064.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2640 a         2685 c         2636 g         2693 t
ORIGIN      
        1 cacctcacct acaattaaaa acacgacatc cgcaccataa atagacttgc aaaaaatata
       61 acatcattgt tttcaatctg ccgtttatgg gattgaccgt tttcttttga cacggagttc
      121 aacaatgttc ggcataatta tatctgtcat cgtattaatt acgatgggct atttgatcct
      181 gaaaaactac aaacctcagg tggtgctggc tgccgcaggt atcttcctga tgatgtgcgg
      241 tgtctggtta gggttcggtg gtgtactcga tcccgccaaa agcaacggct acttgatcgt
      301 cgatatttat aatgaaatcc tgcgcatgct gtccaaccgc attgccggat tggggctatc
      361 gattatggcg gtgggcggtt atgcccgcta catggagcgc ataggggcca gtcgcgcgat
      421 ggtgagcttg ttaagccgcc cgttaaaact cattcgctcg ccgtatatta ttctgtcggc
      481 aacttacgtc atcggtcaaa tcatggcgca gtttattacc agcgcctccg gtctgggtat
      541 gttgctgatg gtcaccttat ttccgacact ggtgagtctg ggagtaagtc gtctctctgc
      601 ggtggcggtt atcgcaacca caatgtccat tgagtgggga attctggaaa cgaactccat
      661 ttttgcagcc caggtcgcgg gaatgaaaat tgccacttac ttcttccact accagcttcc
      721 ggtcgcctct tgcgtcatta tctcggtggc gatctcccac tttttcgtgc aacgcgcttt
      781 tgacaaaaaa gataaaaata tcaatcacga acaagcagag caaaaagctc tcgataatgt
      841 cccgccgctc tattacgcca ttttacctgt gatgccgtta atcctgatgc tcggctcgct
      901 gttcctcgcc cacgtcgggc tgatgcagtc agaactgcat ctggtggtgg tgatgttact
      961 gagtttgacg gtgacgatgt ttgttgagtt cttccgcaag cataacttgc gcgaaacaat
     1021 ggacgatgtg caggcgtttt ttgacggcat gggtacgcag tttgccaacg tggtaacgct
     1081 ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg attggcactg tcgatgcggt
     1141 tatcaggggt gcggagcatt ctggtctggg cggtattggc gtgatgatta ttatggcgct
     1201 ggtcattgcc atttgtgcca ttgtgatggg ctctggcaat gcgccgttta tgtcatttgc
     1261 cagtcttatt ccgaatatcg cagccggact acatgtacca gcggttgtaa tgattatgcc
     1321 gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg attactgcgg tggtggtcgt
     1381 tacgtcagga attgcaggcg tttcgccttt tgcggtggtg aagcgaacag cgatccccat
     1441 ggcagtcggt ttcgtggtga atatgattgc cacaatcacg ctattttatt aagccattaa
     1501 aagacaaaac aggccaccag ggcctgtttt tgtattactt cacaacgcgt aatgccggtc
     1561 gaccaccgcg tggtggctgc ggaggttcat cgtcaggatg agtgtcatca tcgtgatctg
     1621 gcttgtcgcc atcaataacc gacataacgg tttcgttgtc tgccgatgcc tcttcatcat
     1681 tcatgatgct ggtatcttca tcgtaggcag cttcaggctc aaacattgtg cctgcgccat
     1741 tttcacgggc gtagatagcc agcacggcag ccagcggcac agaaacctga cgcggaatgc
     1801 cgccaaagcg cgcgttaaag cgcacctcat cattcgccag ttccagattg ccgacagcac
     1861 gcggcgcaat gttgagtacg atttgtccgt cacgcgcata ttccatagga acctgcacgc
     1921 cagggagcgt cacatccacc accaggtgcg gcgtgagctg gttatccagc aaccactcat
     1981 agaatgcacg cagcagatag ggacgacgtg gtgttagctg tgacaaatcc atacagatta
     2041 actccggccc agacgcattt cacgttctgc ttcagttaaa gaagcaagga aagagtcacg
     2101 ctcaaagacg cgggtcatat agcctttcag ctctttcgca cccgggccgc tgaactcgat
     2161 gcccagttgc ggcagacgcc acagcagcgg agcaagatag caatcgacca ggctgaactc
     2221 atcgctcagg aagtacggct tctgaccgaa gaccggcgca atcgccagca gttcttcgcg
     2281 cagttgctta cgtgcggcat ctgcttcaga agctgaaccg ttgatgatgg tgttcatcag
     2341 cgtgtaccag tctttttcga tacgatgcat gtacagacgg ctttcaccgc gagctaccgg
     2401 gtaaacaggc atcagtggcg gatgcgggaa acgctcatcc agatattcca taatgatgcg
     2461 agattcccac agggtcagct cacgatccac cagggtcgga acgctctgat tcgggttgag
     2521 gtcaatcaga tcctgaggcg gattgtcctt ttccacgtgt tcgatctcga aacttacacc
     2581 tttctcagcc agcacaatgc ggacctgatg gctatagatg tcagtaggac cggaaaacag
     2641 cgtcattacc gaacgtttgt tggcagcgac agccatgaaa acctccaggt atagtcagaa
     2701 tttttactgc taccagccac caggtggcca gtcagaagtt gtgttaccca gtaaggaacg
     2761 actctctttg ttcgaaaatc aaacaaaaaa tgagcaatac ccgacatttg ggcagaaaat
     2821 tggatgatag tttaccagat tttgcgacct ttgtggtgag tcgatgccgg aaatggggaa
     2881 aaagagatgc gctttagtct gaaatagttg acttagtccc ttattggcga tgtggttttt
     2941 gttttgcctg tctgtcagac ggtagcaaaa agcaactttc caatttttac gctgattcag
     3001 aatttaacca taaaaaaacc cgccgaagcg ggttttttcg caaattgttt tctgccggag
     3061 cagaagccaa ttaacgtttg gagaactgcg gacgacgacg tgctttacgc agaccgactt
     3121 tcttacgttc aacctgacga gcgtcacgag taacgaagcc agctttacgc agttcagaac
     3181 gcagggactc gtcgtattcc atcagagcgc gggtgatacc gtgacggatc gcaccagcct
     3241 gaccagagat accaccacct ttaacggtga tgtacaggtc cagtttctca accatgtcga
     3301 ccagttccag cggctgacga actaccatgc gggcagtttc acgaccgaag tactgttcca
     3361 gagaacgttg gttgattacg attttaccgt tgcccggttt gatgaaaacg cgagctgcgg
     3421 aacttttgcg gcgaccagtg ccgtagtatt gattttcagc cattgcctat aatcccgatt
     3481 agatgtcaag aacttgcggt tgctgtgccg cgtggttgtg ctcgttaccc gcgtaaactt
     3541 tcagtttacg gaacatagca cgacccagcg ggccttttgg caacatgcct ttaaccgcga
     3601 tttcaatcac acgctcagga cggcgagcaa tcatctcttc aaaggtcgct tgtttgatac
     3661 caccgatgtg gccggtgtgg tgatagtaca ctttgtcagt acgcttgttg ccggttacag
     3721 caactttgtc agcgttcaga acgatgatgt aatcaccggt atctacgtgc ggagtgtatt
     3781 ccgctttgtg cttaccgcgc aggcgacgag ccagttcagt agccagacgg cccagagttt
     3841 taccggtcgc gtcaacaaca taccagtcgc gttttacggt ttctggttta gctgtaaaag
     3901 ttttcattaa aagcttaccc aataaatagt tacacgttgg tgaacaccca aacgtcttca
     3961 attgttgagg ttcacacgac aaagtccggc aaacctaccc cttcgaatag cctatgccag
     4021 cacacaaaaa gttttgggaa aaaaactttc ttgtaacgtg gggtcgcagg attatagaga
     4081 agtcggggtc aaagatcgac ccctttttgt gatttgtgac aggttttaac ccgccaaatg
     4141 ctcgcgcttc agatactctt cgctttgcat ctcttgcaga cgtgacaggc aacgctggaa
     4201 ctcaaacttc agccgatcgc cctgataaat ttcatacagc ggcacttctg cactcaccac
     4261 taatttgaca tggcgctcgt aaaactcatc caccagcgca ataaagcgcc gcgcttcgct
     4321 ctccatcaac cgcgtcataa ctggtacatc aaacaacatg accgtatgaa agaggcgtga
     4381 gagcgcaata tagtcatgct ggctgcgggc atcgacacac aaggtggtaa aagagacagc
     4441 aagcgtctga ttctcagcgc ccattgtcgc cagcggtcga tggttgattt ctaacgtcgg
     4501 tgaattttcg cgtttcgctc ccgccagcgc caaccatagt ttatccattt gtgcccgggt
     4561 ttcatcgtgg agtggcgaaa gccacagatg cgcctgagtg agtgtacgca gtcgataatc
     4621 aacaccagcg tccacgttca ttacatcaca atgctgttta atggcatcga ttgcaggcag
     4681 aaaacgcgca cgttgcaagc catttcgata aagttcgtcc ggcggaatat ttgacgtcgc
     4741 taccagggta ataccgcggg cgaacagggc tttcatcaga ccgccaagta gcatggcgtc
     4801 ggtaatatca gaaacaaaaa attcgtcaaa acagagcacg tcagtttcgg ctttaaagcg
     4861 atcggcaata atttccagcg gatcggtctg tccctgtaag gcagttagct cttcgtgcac
     4921 ccgcagcata aaacggtgaa agtgcaggcg ctgtttccgc tctcccggca ggctttgata
     4981 gaaaaggtcc atcagccagg ttttcccgcg tcctacaccg ccccacatat ataagccacg
     5041 cactggcgta tgctttgtat cttcgcgttt accccatagc ttaccgaccc gcgccattag
     5101 cccactcgtc ctgggggctg gtggcctgct attgatgagt tcttgataaa taatttccag
     5161 gcggctgacg gcctcttttt gaacgtcgtc gggttgatgg ctgccttcat tgagcgcctt
     5221 caggtattgc gatgttgggg taacgctttg catgatctta ttgttattcc ttgaataatc
     5281 ggtgcgccgt tgttcacggt tgacgaaaaa aaggccgttc tacactacgc gatatgcagt
     5341 cgggattcca cttctgtgga attaacggtt atagtggcat aatcagccgc aggcatggag
     5401 cctgaagcca acaccctacg gaaacaaaag acaacgggag atgttcatga cctgggaata
     5461 tgcgctaatt gggttagtcg tcggcatcat tattggtgct gtggccatgc gttttggtaa
     5521 tcgtaaacta cgccagcaac aggcgttgca gtacgaactg gaaaagaata aagctgaact
     5581 ggacgagtat cgcgaagagc tggttagcca ctttgcccgc agcgcggaat tactggatac
     5641 catggcgcac gactatcgcc agctgtatca gcacatggcg aaaagctcca gcagcctgct
     5701 gccggaactg tctgctgaag caaacccgtt ccgtaatcgt ctggcagagt ctgaagccag
     5761 caacgatcag gcaccggtgc agatgcctcg cgactattct gaaggcgcat ccggcctgct
     5821 gcgtactggc gcgaagcgcg actaatttat ttttcgggcg cagccattgc gccctcctct
     5881 tctctccctc cccgactatc atttaatgtg gtgtctcatt gttagccgtc tgaaaattca
     5941 ataacatcaa actgttttga atctcttttc ttatcattca ggtacgagag caggaataat
     6001 gaaaaaacaa acccagctgt tgagtgcatt agcgttaagt gtcgggttaa ctctctcggc
     6061 gtcatttcag gccgtcgcgt cgattccagg ccaggttgcc gatcaggccc ctctccccag
     6121 tctggctcca atgctggaaa aagtgcttcc ggcagtggtg agcgtacggg tggaaggaac
     6181 ggccagtcag ggacagaaaa tcccggaaga attcaaaaag ttttttggtg atgatttacc
     6241 ggatcaacct gcacaaccct tcgaaggttt aggctccggt gtcatcatca acgccagtaa
     6301 aggctatgtg ctgaccaaca accatgtgat taatcaggca cagaaaatca gtattcagct
     6361 caatgatggg cgcgagtttg atgcaaaact gattggtagc gatgaccaga gcgatatcgc
     6421 cctgttacaa attcaaaacc cgagcaaatt aacgcaaatc gctattgccg actccgataa
     6481 attgcgcgtc ggtgattttg ccgtagcggt cggtaaccca tttggccttg ggcaaaccgc
     6541 cacctctggc attgtttccg cattaggacg cagcgggttg aatcttgaag gtctggaaaa
     6601 ctttatccag acagatgctt ccattaaccg cggtaactcc ggcggtgcac tgttaaacct
     6661 taacggtgag ttaattggca tcaacactgc aatccttgcg cctggcggcg ggagcgtcgg
     6721 gattggattt gccatcccca gtaatatggc gcgaacactg gcgcagcagc ttatcgactt
     6781 tggtgaaatc aaacgcggtt tgttaggcat caaagccacc gagatgagtg ccgatatcgc
     6841 caaagccttc aaccttgacg tgcagcgtgg cgcgtttgtc agcgaagtgt tgccaggttc
     6901 tggctcggca aaagcgggcg tcaaagcggg cgatattatt accagcctca acggcaaacc
     6961 gctgaatagc tttgctgagt tgcgctctcg tatcgcgacc accgagccgg gcacgaaagt
     7021 gaagcttggc ctgctgcgta acggcaaacc actagaagta gaagtgacgc tcgataccag
     7081 cacctcttcg tcggccagcg ctgaaatgat cacgccagcg ctggaaggtg caacgttgag
     7141 cgatggtcag ctaaaagatg gcggcaaagg tattaagatc gatgaagttg tcaaaggaag
     7201 cccagctgct caggctggct tgcaaaaaga cgatgtgatc attggcgtca accgcgatcg
     7261 ggtgaactcg attgctgaaa tgcgtaaagt gctggcggca aaaccggcca tcatcgccct
     7321 gcaaattgta cgcggcaatg aaagcatcta tctgctgatg cgttaatgtc gtaaaccggg
     7381 catcaggctt acgtgtgatg tccggttaac tcgtggtatg ctgctgccgt tccctttttt
     7441 aatgacgcct ccatcatgtt tgtgaagctc ttacgttccg ttgcgattgg attaattgtc
     7501 ggcgctattc tgctggttgc catgccttcg ctgcgcagcc ttaacccgct ttccactccg
     7561 caatttgaca gtaccgatga gacgcctgcc agctataatc tggcggttcg ccgcgccgcg
     7621 ccagcggtgg ttaacgttta caaccgtggt ttgaacacca actctcacaa ccagcttgag
     7681 atccgcaccc tgggatccgg tgtaatcatg gatcaacgcg gttatatcat caccaataaa
     7741 cacgtcatca acgacgccga tcagatcatc gtcgccttac aggatggacg tgtatttgaa
     7801 gcattgctgg tgggatctga ctctctaacc gatctggcgg tacttaaaat taatgccact
     7861 ggcggtttac ctaccattcc aattaatgca cgtcgcgtac cgcacattgg cgacgtagta
     7921 ctggcgatcg gtaacccgta caacctcggg cagaccatta cccaggggat tattagtgcc
     7981 acgggtcgaa tcggtctgaa cccgaccggg cggcaaaact tcctacaaac cgatgcttcc
     8041 attaaccacg gtaactctgg cggcgcgctg gtgaactcgc tgggagaact gatgggcatt
     8101 aacacgctgt cgtttgataa gagtaacgat ggcgaaacgc cggaaggtat cggctttgcg
     8161 attcctttcc agttagcaac caaaattatg gataagctga tccgcgatgg tcgcgtgatc
     8221 cgcggctaca ttggtatcgg cggacgtgag atcgcaccac tgcacgcgca gggcggtggt
     8281 atagatcaac tgcaagggat cgtggttaat gaagtgtcac ctgacggccc ggcggcgaat
     8341 gcgggtattc aggtcaacga tctgattatt tcggtggata acaaaccggc catctctgct
     8401 ctggagacga tggatcaggt ggcagaaatt cgccctggtt cggtgatccc ggttgtagtg
     8461 atgcgtgatg ataagcagtt aacgctgcag gtcaccattc aggaatatcc ggcaaccaat
     8521 taagtcgagt gctcaaaaca aaaaaccgga gtctgtgctc cggtttttta ttattcgcta
     8581 attaattact tattaacgaa ctcttcgccc agggcgatat ctttcttcag cgtatccagc
     8641 ataccttcca gtgcgctctg ttcaaatgcg ctcagggtac cgatagattt acgctcttcc
     8701 acgccgtttt tacccagcag cagcggttga gagaagaaac gtgcgtactg accgtcgcct
     8761 tcaacatagg cacattcgac aacgccttgt tcgccctgca gtgcgcgtac cagagacaga
     8821 ccaaaacgtg cagctgcctg gcccatagac agggttgcag acccgccacc ggctttcgct
     8881 tcaaccactt cagtacctgc gttctggata cgtttagtca gatcagccac ttcctgctcg
     8941 gtaaaactaa cgccaggaac ctgtgacagc agcggcagaa tggtaacacc agagtgaccg
     9001 ccaataaccg gcacttcaac ttcgcctggc tgtttgcctt tcagttccgc aacaaaagtg
     9061 ttggaacgaa tgatatccag cgtggtaacg ccgaacagtt tgtttttgtc ataaacaccg
     9121 gcttttttca gcacttcagc agcaattgca acagtggtgt taaccgggtt agtgataata
     9181 ccaatgcacg ctttcgggca ggttttcgca acttgctgta ccaggttttt cacgatgccg
     9241 gcattaacgt taaacaggtc ggaacgatcc atacccggtt tacgcgctac acctgcagag
     9301 ataagaacga catctgcgcc ttccagcgcc ggagtcgcat cttcaccaga aaaacctttg
     9361 attttcacag cagtagggat atggctcaga tcgacggcca caccgggagt cactggagcg
     9421 atatcataca gagagagttc tgaacctgaa ggcagttggg tttttaacag tagtgcaagc
     9481 gcctggccaa taccgccagc agcgccgagg actgcgactt tcatcctaaa ctccttatta
     9541 tattgataaa ctaagatatg ttgctccgct gccgcgacct taatccacaa aattgccgtt
     9601 tacaatgacc acatctcaag aatgtgtagt cacgcaagtt tagcgtttat gcatttaatt
     9661 gccgtaatca ggaacctaac ttacgtaatt aacagtcgct tatgcattag cgcaacattc
     9721 caacaggtgg tgacaatata ccctaccgtt cagccaaaac aacatcaatt tgataacaat
     9781 taatttactt ttaagcagaa tttgcatgcc gtgacgcagg catgtttctc aataacgaaa
     9841 tttgataaaa tcccgctctt tcataacatt atttcagcct tcttcagggc tgactgtttg
     9901 cataaaaatt catctgtatg cacaataatg ttgtatcaac caccatatcg ggtgacttat
     9961 gcgaagctcg gctaagcaag aagaactagt taaagcattt aaagcattac ttaaagaaga
    10021 gaaatttagc tcccagggcg aaatcgtcgc cgcgttgcag gagcaaggct ttgacaatat
    10081 taatcagtct aaagtctcgc ggatgttgac caagtttggt gctgtacgta cacgcaatgc
    10141 caaaatggaa atggtttact gcctgccagc tgaactgggt gtaccaacta cctccagtcc
    10201 attgaagaat ctggtgctgg atatcgacta caacgatgca gttgtcgtga ttcataccag
    10261 ccctggcgcg gcgcagttaa ttgctcgcct gctggactca ctgggtaaag cagaaggtat
    10321 tctgggcacc atcgctggcg atgacaccat ctttaccacc cctgctaacg gtttcaccgt
    10381 caaagacctg tacgaagcga ttttagagct gttcgaccag gagctttaat ctctgccccg
    10441 tcgcttctgg cggcggggaa aatgttgctt atccctctca ttccccttct ttctcctgcg
    10501 atacatttaa cgaatagtgc gttttaccgc gacacatcat tcacaaaatg aatacatacg
    10561 gtaaaaaaag cacattatgc aaaattcatt atctaattga aaatattata attaataata
    10621 aataaccgtg ttttttatac ttttttgtta ttaa
//
LOCUS       DAZKIV010000155        10160 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZKIV010000155 DAZKIV010000000
VERSION     DAZKIV010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10160
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.155"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..616)
                     /gene="yncG"
                     /locus_tag="ACHOT6_003917"
     CDS             complement(<1..616)
                     /gene="yncG"
                     /locus_tag="ACHOT6_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFP9949065.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            883..2382
                     /gene="ansP"
                     /locus_tag="ACHOT6_003918"
     CDS             883..2382
                     /gene="ansP"
                     /locus_tag="ACHOT6_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFP9949066.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHOT6_003919"
     CDS             complement(2495..3556)
                     /gene="yncE"
                     /locus_tag="ACHOT6_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFP9949067.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHOT6_003920"
     CDS             3798..5900
                     /gene="pqqU"
                     /locus_tag="ACHOT6_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFP9949068.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTSVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGVPEYGQKGELRRDERN
                     LMWNVDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHOT6_003921"
     CDS             complement(5936..6601)
                     /gene="mcbR"
                     /locus_tag="ACHOT6_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFP9949069.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHOT6_003922"
     CDS             complement(6799..7836)
                     /gene="curA"
                     /locus_tag="ACHOT6_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFP9949070.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8017..8535
                     /gene="mddA"
                     /locus_tag="ACHOT6_003923"
     CDS             8017..8535
                     /gene="mddA"
                     /locus_tag="ACHOT6_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFP9949071.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHOT6_003924"
     CDS             8532..8981
                     /gene="ydcZ"
                     /locus_tag="ACHOT6_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFP9949072.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHOT6_003925"
     CDS             complement(8982..9215)
                     /gene="ydcY"
                     /locus_tag="ACHOT6_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFP9949073.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEACHEQLTKGGTIL"
     gene            complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHOT6_003926"
     CDS             complement(9301..9474)
                     /gene="ortT"
                     /locus_tag="ACHOT6_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFP9949074.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9669..9764
                     /gene="yncL"
                     /locus_tag="ACHOT6_003927"
     CDS             9669..9764
                     /gene="yncL"
                     /locus_tag="ACHOT6_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFP9949075.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2455 a         2491 c         2475 g         2739 t
ORIGIN      
        1 aaataatttc attccttttt aatacttctt gtaatttcgg taactgacat acagcatcag
       61 caatcgcact gatattcgtg gcattatcct gaaaccattc atgcccaggc ccccatgtgc
      121 gcatagtgca aagataacag tccaccagcg ttagttgctc accaaacgca taaggttcag
      181 cggtcaattg tgaattcagc cagatataaa gcgatttacg atattcaata acattcttct
      241 ttaactgttc aggtgcgtca ggagcccaac gctcagggta atcggcgaaa gtgaatgttg
      301 gatagacatt agcaaccagc cagaccaata gccgttgaaa ctgctgacgt tcggcgcgcc
      361 caaccggtgg cgcaaggtcc gggcgacgat cgagaaccat caatgcaatc gccgctgtct
      421 ccgtcataat ctcgtcattt tccagcgcca gtgtcggaat ctgacacaac gggttcaggg
      481 ttttcaataa ctcgcgtgaa gttccctcgt ggtcaaaacc actgacatca acgaactgat
      541 aaggaatatc agccagtgtt agcatcagct cactgattgt agagccccat ccgggtacgc
      601 catacacctt aatcatatgc ccctcacaat cttttaaaga ttaagtgtag gacagtacac
      661 aggttaacca gcaaaaggat ttgtcatttt ttcgtcagtt gtattaatcc ccttcaggat
      721 tgtaagggga tgttttgcat cgtttatcga taacgggtag gatgctgcgc cgatagtttt
      781 atacccataa agaataatgg tgataactat catcgccagg atgaataaac attgttcatg
      841 gcaacttata tgactttttc attaaagcaa tcagggagag caatgagtaa acacgacacc
      901 gacacttcag atcaacacgc cgcgaaacgc cgctggctta atgcccacga agaggggtat
      961 cacaaagcga tgggcaatcg ccaggtacag atgatcgcca ttggcggcgc gattggcacc
     1021 ggcttgtttt taggtgcagg agcccgactg caaatggcgg gtccagcact ggcactggtt
     1081 tatttaattt gtggcttgtt ttcgtttttt attctgcgtg cattgggtga gctggtgcta
     1141 caccgccctt ccagtggcag ttttgtctct tatgcccgtg agtttttggg tgagaaagcc
     1201 gcttatgttg ctggctggat gtacttcatc aactgggcga tgacggggat tgttgatatt
     1261 accgccgtcg ctctgtatat gcattactgg ggtgcgtttg gcggcgtgcc gcagtgggtc
     1321 tttgcgctcg ctgcgctgac catcgttggc accatgaata tgatcggtgt gaaatggttt
     1381 gcggagatgg agttctggtt tgcgcttatt aaagtgctcg ccattgtgac ctttttggtc
     1441 gtgggtacag tgttcctcgg tagcggtcag ccgctggatg gcaacaccac tggctttcat
     1501 ttaatcaccg ataatggcgg cttcttcccc cacggtttgc tgcctgcgct ggtgttgatt
     1561 cagggcgtag tgtttgcttt tgcctccatt gaaatggtgg gtacagctgc cggagaatgt
     1621 aaagatccgc agaccatggt gcctaaagcg attaacagtg tgatttggcg tattggcctg
     1681 ttttacgtcg gctccgtggt gttgctggtt atgttattgc cgtggagcgc gtatcaggcg
     1741 gggcaaagtc cgttcgtgac gtttttctct aaactgggtg tgccatatat cggcagcatt
     1801 atgaacattg tggtgctgac cgctgccctc tccagcctga actcaggcct gtactgcacc
     1861 gggcgtattc tgcgctcgat ggcgatgggc ggttccgcac cgagttttat ggcgaaaatg
     1921 agtcgtcagc atgtgccgta tgccgggatt ctggcgacac tggttgtgta tgtcgtcggc
     1981 gtattcctca actatctggt gccgtcgcgc gtatttgaga ttgtgttgaa cttcgcgtcg
     2041 ctgggaatca tcgcttcatg ggcgtttatc atcgtgtgcc agatgcgcct gcgtaaagca
     2101 attaaacaag gcaaagcagc ggatgtcagt tttaaactgc ctggcgcgcc cttcacttcc
     2161 tggctgacat tactgttttt actgagtgtc ctggtgctga tggcgttcga ttacccgaac
     2221 gggacttata ctatcgcggc gctgccgatt atcggtattc tgctggttat aggctggttt
     2281 ggtgtgcgca aacgcgttgc tgaaattcac agcactgcgc cagtcgtcga ggaagatgaa
     2341 gaaaaacagg aaattgtgtt taagcctgaa acggcgagtt aattgcaaaa ttgcccggct
     2401 acgtgccggg caaattcaga ttactgacga aggtgccgga tgcggcgtga taaaagtcag
     2461 gcaagtctct ccgttttcac cctcgccata tctattacag cgcaatacga atcacatcgt
     2521 ctggctgggt agcttcctgc tgtttagtgg atttttgttt cacactgaca tacagcgttt
     2581 tgccatcggc agacagcgcc aggctgtttg gatgagtcgg cgtatcgaac gttttcacca
     2641 ctttatagct tttcgcgtca atcacactaa ctttacctgc ctgacgatgc gttacgtagg
     2701 cttcattacg cgctgggtta aacagcacag ccagtgattc cggtgccgca accttcgcca
     2761 gaatattgcc attacgggta tcgaccacta acacttctgc ggctttagaa tcggtgataa
     2821 atgcacgctg cctggcggta tcaaggctaa tgttgataaa gaagtgctct ttgccgtcat
     2881 ccagcagctt tttacggctg aggattttat tgtcggcggt gtcgatggta atcaattcgc
     2941 cgtcagcgtt agtggtgtaa agacgtttgc ctttgctatc cagcgccaga ccggtactca
     3001 ttttaccggt gttctggatg gcggttttca gtttgatatt ctcgccatca acgacccaaa
     3061 tcacgctatc tttaccaata ccactgatgt aaacggtgtt cgtggcatca tcagctacca
     3121 gctcacgcgg ttgcagcggg cgcacctctt ccgtgcgctt acgatcatcc agcaccagac
     3181 ggcctttcac ttcgcccgtt ttggcatcta tcgccgtgac cgcgctgttt acggtgttac
     3241 caaaccacaa cgtctgagtc gtgttattga tggtggcacc aaacggcttg agatcgttat
     3301 ggatcgcctg cgtcacttcc agagtgaccg gatcaagacg ataaaccacg ccacctttat
     3361 ccagtttgcg gctttgcgaa gtggcgagcc acagcgcgtt ttcttgctgg ctataagcca
     3421 tttcgtaggc acctttacct accgctttac gcagcatttc ttctgcggcc tgcgtactga
     3481 atgatgaagc aacaagcaat gaacctaaca gtaatgaacc acgcaggcgc gatgaaaaca
     3541 gatgacgtaa atgcatgacg actccctttg atgaaccgat atttttgctg cttcacaatg
     3601 cttcgctaag cgtaatcatc gttacgctct tgttataaat gggaatggta atcattattt
     3661 tctcaaatgt ggagcatttc ttgccgttac gactacaatt ttctcaatta cttaacgtat
     3721 tgtgccgttt cattttccat aaatttacaa tttcattacc atgacggcgt tttcccattc
     3781 ttaacctggc ttttttcatg aagatttttt ccgtccgaca gaccgttttg cccgcactgc
     3841 ttgtcctttc ccccgttgtt tttgccgctg atgaacagac catgattgtc agtgccgcgc
     3901 cgcaggtggt ttccgagctg gatacgccag cagcagtaag cgtggtggat ggcgaggaga
     3961 tgcgcctggc aacaccgcgc attaacttgt ccgaatcact gaccagcgtg cctggtttgc
     4021 aggtacaaaa ccggcagaac tatgcgcaag atttacagct gtcgattcgc ggatttggct
     4081 cccgctccac ttacggaatt cgcggtattc gcctgtatgt ggacggtatt cccgccacca
     4141 tgcccgacgg gcaagggcaa acatccaaca tcgatttaag cagtgtgcaa aatgtggaag
     4201 tgctgcgtgg ccccttctct gccctgtatg gcaacgcgtc tggcggtgta atgaatgtca
     4261 ccacccagac cggacaacag ccaccaacca ttgaagccag tagttactac ggcagttttg
     4321 gcagctggcg ctatgggctg aaagcaaccg gcgcaacggg cgacggcaca cagcccggcg
     4381 atgttgatta caccgtctca accacgcgtt ttacgaccca cggctatcgt gaccatagtg
     4441 gcgcacagaa aaatttagcc aatgccaaac tgggcgtacg cattgatgaa gccagcaaat
     4501 taagcctgat tttcaatagt gtggatatca aagcagatga ccctggtggg ctaaccaaag
     4561 cagaatggaa ggcgaatcca caacaagcgc ctcgtgcaga acagtacgac acgcgaaaaa
     4621 ccatcaagca aactcaggct gggttgcgct atgagcgtag cctgagcgcg caagatgata
     4681 tgagtgtgat gatgtatgcc ggagagcgag aaacgaccca gtaccagtca atcccgatgg
     4741 ccccccaact taacccgtcg catgcgggcg gcgtgattac cctgcaacgc cattaccagg
     4801 ggatagacag ccgctggaca caccgtggtg aactgggcgt tccggtcacg ttcactaccg
     4861 gcctgaacta cgaaaacatg agtgaaaacc gcaagggcta caataacttt cgcctgaata
     4921 gcggcgtgcc ggaatacggg caaaaaggtg agttgcgtcg cgacgaacgc aatctgatgt
     4981 ggaacgtcga tccctattta cagacgcagt ggcagctgag cgaaaaactg tcgctggatg
     5041 ctggcgtgcg ctacagctcc gtgtggtttg attccaacga tcattacgtt actccgggta
     5101 acggcgatga cagcggtgat gccagttatc acaaatggct acctgccggt tcgttaaaat
     5161 atgcaatgac cgatgcctgg aatatctatc tggcagccgg gcgtggtttt gaaacgccga
     5221 cgattaatga gctgtcttat cgcgctgacg ggcaaagcgg tatgaacttt ggtttaaaac
     5281 catccaccaa cgatacaatt gagatcggca gtaaaacgcg tattggtgat gggctgctta
     5341 gtctcgcatt gttccagacc gacaccgatg atgaaattgt tgtcgatagc agtagcggtg
     5401 ggcgtacgac ttacaaaaat gccggaaaga cccgtcgtca aggcgctgaa ctggcatggg
     5461 atcaacgttt cgcgggagat tttcgcgtaa acgcgtcctg gacctggctt gatgcgacct
     5521 atcgcagcaa tgtttgcaac gaacaggatt gtaacggtaa tcggatgcca gggatcgccc
     5581 gtaatatggg ctttgcgtcg ataggttatg taccggaaga tggctggtat gcaggcacgg
     5641 aagcgcgtta tatgggcgat attatggcag atgatgaaaa tacggcaaaa gcgccgtctt
     5701 atactctcgt cggcttattc accgggtata aatacaatta ccacaattta actgtggatt
     5761 tatttggtcg tgtcgataat ttattcgata aagaatacgt tggttctgtc attgtcaatg
     5821 agtcaaacgg gcgatattac gaaccttcgc ccggacgaaa ttatggtgtc ggcatgaata
     5881 ttgcgtggag atttgagtaa ttaccttgat gcccggtatt tgccgggcat ttactttaac
     5941 gattgtattg ctggtataaa atagcaatat tttgttgcat aatttcagca agacaatgtc
     6001 tgcatccctc tttgtctttt gcttttaacg cagcgagtaa cagatgatag ttttctatat
     6061 gctcccgtaa ttccgctgga ttaatcgctt catagagata atgtaaacca ggccccatcc
     6121 tgacccacag ttgctcaatc atctcacaca ggatgggcat atttgagcga tgataaatcg
     6181 ctaagcgaaa tagcctgttt acattaatga tttgttccat gtcgcccttt tcttgcgcct
     6241 gctgtaattt ctcgagcaat tcctggagtt ccgccaggtc ttgcggggtg aggttttcaa
     6301 cagccagagc aactgccatt aattccagtt cgtagcggat ccgattgatt tcgtctaatt
     6361 gacgtttccc cacttccgga actgtaaatg cttgtgcagg tgcgacagaa agcgcattca
     6421 ccgaaaccag acgtaataat gcttcacgca caggtgtaat actcatacct aattgctccg
     6481 ccagattttt agtaatcagg cgtgcgccag gttttaatgc gccaatacta agctgatgtt
     6541 tcaggtcgtt ctcaacctgc agagtcaaac tgacatgttt catttttccc attccaggca
     6601 ttttaattcc ttcgcgtgtt ttctgtccaa ccctgcctgc acggcagaag gagttcacct
     6661 ggtgtattaa agtgaataat cataaaccac ccacctttgt tgttattagc agcagaacaa
     6721 tgacgtggtt agttaattaa agctgaaaat aagctttttg cctggatttt tatggcggct
     6781 tacgccgccg ttgaaacttt aatcatcacc cgcgacgcgg atcaccactt tgccgaagtt
     6841 tttacccttc agcaggccga taaacgtctg tggcgcattc tctaaaccat cagtaatatc
     6901 ttcgtggtag tggattttat cctctttcac ccattgcccc atctccttct gaaactcatg
     6961 gatgcggtga ccataatcct gagcgataat aaacccttgc aagcgaatac gttttttcag
     7021 caccgttgcc atcaacaggg gtaaacgatc cggaccgggt ggtagctctg tagcgttata
     7081 gctgctcact aagccacaaa cgggaatgcg cgcagatgta ttaagtaatg gtagcactgc
     7141 atcaaatacc ttaccgccca cgttttcata atagatatca ataccttttg ggcacgcttt
     7201 caccagttgt tcggcaaaat catccgcgtg gtgatcaaga caaacatcga agcccagcac
     7261 ctcgatagca tggcggcatt tttccgcgcc accggcaacc cccaccaccc tgcaaccttt
     7321 aagtttgccg atttgcccca ccgttgctcc cacaggacct gtcgccgcag ctaccaccaa
     7381 cgtttcgccc tctttaggct gaccgatatc cagcagcccc atataagcgg taaagcctgg
     7441 catccccagc acacccagcg accacgatgg attttgcgga tgatcgccaa gtttcaccag
     7501 atcatcacca ctggatatgt catagtcctg ccatccactg tagcccagca cccagtcgcc
     7561 aggctgataa tcaggatgat tcgactccac aacacggctc accgtgccgc cgaccatcac
     7621 gccgccaata tcaacaggcg gtgaataaga tggctcatcg ctcatacgtc cacgcatata
     7681 cgggtccagg gacaaataaa ctgtgcgcag taacacctgt ccttcacccg gtgtggcgac
     7741 atcatcttct tcaagacgga aattctccgg aaccggtgcg ccatgtggac gcgaggccag
     7801 aacccaacgt cgattacgct gcttttgttg ccccataatg ctctcctgtg cttttggcat
     7861 gaatccatca atagtagcag gctcacggga ttggttttgt ttgcagccgt cacatcccct
     7921 ctgcaacacg caataatttc atcgaggaat ccgttgcttc gggctgtttt cgatagtatt
     7981 gataacatca tcatcattat tttatcgagg ccagacatgt ccatccgttt tgcccgcaaa
     8041 gccgattgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac ggcggctatc
     8101 tggaatgacc aaacggttga tgcagataac cgcattgcct ggtttgaagc gcggaccata
     8161 gcaggttatc cggtgctagt aagtgaagaa gatggcgtag tgacgggata tgcctcgttt
     8221 ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt ttatgtccat
     8281 cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat tgatgaagcg
     8341 cgggatagcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca ggcctcgttg
     8401 catctgcacc attcgctggg atttgtcgtc accgcacaaa tgccgcaggt gggcactaaa
     8461 tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg cactgaaccg
     8521 gacgcgattg gatgaatcag tcgctcaccc ttgctttttt gattgccgcc ggaattggtc
     8581 tggtagtcca gaacacgctg atggtgcgca tcacccagac ctcctctacc attctcatcg
     8641 ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta tggtttaaac
     8701 aaggcatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca cttatacccg
     8761 gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat gtgggagcgg
     8821 cgacaactat tgcagtactg gtcgccagcc agctaattgg cggtctgatg ctggatatct
     8881 tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc gcaattttac
     8941 ttgtggttgg cgcctggttg gtagccagac gctcatttta atcagaggat ggtgccgcct
     9001 ttggttaact gttcgtggca cgcttccata tcttctttat gcttgcgacc gtgatgagca
     9061 atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa ctccgcgtca
     9121 tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc aatcgcggca
     9181 tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttaacc acttgtgaaa
     9241 tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag tgagtaagat
     9301 ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg cccccaccag
     9361 aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccagg tgataagaaa
     9421 tgcgattgct gccataaccg cataaaaaac aagcatatgt tgatagagag acatgtacgg
     9481 gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac gtgcatcaag
     9541 ttttttcacc agtttgagaa aaaatcatca aacagcaact tgcccttatc catcttcaga
     9601 atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa tgataaaagg
     9661 tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt gctgctgttg
     9721 gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt gattttgcac
     9781 gcagcacgta ctgtcagaga aatcaactct ctgacagtac gtaagatcat ctcatactct
     9841 ccaggtttac aacattatcc ttaatattta cttctttttg ctggcatcta cattgaaatg
     9901 agtagaaaac atccccctac tgtcgtctat acaaacagct tactgattca aacggaatct
     9961 tgttttcaca tttttctatc atgaatactt tatacgcgcg ggagtctata aatgctttat
    10021 actttcaagg aataacgtaa atataaaaag atggcgtaaa atatatgcat ggcaggatgt
    10081 ttgcaacctc cttgatgacg ctgccccatt gaaattgcaa accccgccct ggttatcttg
    10141 cccatttatc atgcaaaaca
//
LOCUS       DAZKIV010000156        10136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZKIV010000156 DAZKIV010000000
VERSION     DAZKIV010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.156"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..615
                     /locus_tag="ACHOT6_003928"
     CDS             <1..615
                     /locus_tag="ACHOT6_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP9949076.1"
                     /translation="QSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQMI
                     ALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDYS
                     WIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLI
                     FEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            612..1226
                     /gene="prfH"
                     /locus_tag="ACHOT6_003929"
     CDS             612..1226
                     /gene="prfH"
                     /locus_tag="ACHOT6_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP9949077.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1283..2740)
                     /gene="pepD"
                     /locus_tag="ACHOT6_003930"
     CDS             complement(1283..2740)
                     /gene="pepD"
                     /locus_tag="ACHOT6_003930"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9949078.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3001..3459
                     /gene="gpt"
                     /locus_tag="ACHOT6_003931"
     CDS             3001..3459
                     /gene="gpt"
                     /locus_tag="ACHOT6_003931"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9949079.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3551..4795
                     /gene="frsA"
                     /locus_tag="ACHOT6_003932"
     CDS             3551..4795
                     /gene="frsA"
                     /locus_tag="ACHOT6_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9949080.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRVC"
     gene            4853..5254
                     /gene="crl"
                     /locus_tag="ACHOT6_003933"
     CDS             4853..5254
                     /gene="crl"
                     /locus_tag="ACHOT6_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9949081.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5293..6348)
                     /gene="phoE"
                     /locus_tag="ACHOT6_003934"
     CDS             complement(5293..6348)
                     /gene="phoE"
                     /locus_tag="ACHOT6_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9949082.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6636..7739
                     /gene="proB"
                     /locus_tag="ACHOT6_003935"
     CDS             6636..7739
                     /gene="proB"
                     /locus_tag="ACHOT6_003935"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFP9949083.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7751..9004
                     /gene="proA"
                     /locus_tag="ACHOT6_003936"
     CDS             7751..9004
                     /gene="proA"
                     /locus_tag="ACHOT6_003936"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFP9949084.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9119..9194
                     /locus_tag="ACHOT6_003937"
     tRNA            9119..9194
                     /locus_tag="ACHOT6_003937"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9152..9154,aa:Thr,seq:cgt)
     gene            9801..9959
                     /locus_tag="ACHOT6_003938"
     CDS             9801..9959
                     /locus_tag="ACHOT6_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949085.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         2515 a         2449 c         2642 g         2530 t
ORIGIN      
        1 cagtctattt tacagcggca tattgcctcg ttctcgcatc atggtttgcc tcaaggcagt
       61 gacgacgcgc tacggtatat tgcggaacat gacgatgcgc tggcgtttgc gtgtattaat
      121 cgccagatga tcgctttgcg cataatgcaa caagttaagg ccacgggaag tccggttctg
      181 gatgtggcgc ataactttgt tagcgcgtgt caaatcggcg atcaacaggg ctggttgcat
      241 cgtaaaggtg ccacgccgga tgacaacggt ctggtgatta ttcccggttc acgtggtgat
      301 tactcctgga ttgtacagcc tgttgcgaac gaaaaaacgc tccactcgct ggcgcatggg
      361 gcagggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa atacacggcg
      421 acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa acaactcatc
      481 tttgaagaag cgccacaagc ctataaatcg gctgaaagcg tggtgcaatg tctggtgcag
      541 gccggtttaa ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa aaacagtgga
      601 gggaaaaaag gatgatcttg ctacaactct cctcagcgca ggggccggaa gaatgttgtc
      661 tcgcagtgaa aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa gacgtcgcgg
      721 taacggtgtt ggaaacagaa acgggccgct actctgacac gctgcgttcg gcgctgattt
      781 ctctggatgg cgataacgca tgggcattaa gcgaaagctg gtgtggcact attcagtgga
      841 tttgtccgag tccgtatcgg cctcatcatg ggcgcaaaaa ctggtttctg ggcattgggc
      901 gttttaccgc tgatgagcag gaacaatcgg atgcaatccg ttatgagacg ttgcgttcgt
      961 cggggccggg cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg
     1021 catccggtat tagcgtgaag gttcagtcag agcgtagtca gcatgctaac aagcggctgg
     1081 cacgattgct gattgcctgg aagctggagc agcagcaaca ggaaaatagc gcggtgctga
     1141 aatcgcagcg gcgaatgttc catcaccaga ttgaacgtgg caacccgaga cggacattta
     1201 cagggatggc ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc
     1261 catccggcag caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca
     1321 gcgtccagta atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg
     1381 tgatagttgg cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac
     1441 attccaggcc cgcgtggata atctggatgt tcggtgtttt gttgaacaga cgctgatagg
     1501 tttcacgtac cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc
     1561 ctttcgcttc ggttttcgcg ccagctagtt tacccagcga atccagcatg ctcaccacgt
     1621 agtctttacc gctgtcgatc agtgaacgga tcaggcagtg aatttctacg ttattgtcag
     1681 tcatggtcac cacaccgacg ttcagggagg tttcgaccac accttttgcc acgtcggaat
     1741 tgcggatcac accgttcggg gtggcgttca gcagacgaat aaaggtatcg cgagatttcg
     1801 caatcagggc agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt
     1861 tctctgccag ctcgtttttc aggatctcct gataggtatt caccagagat ttcaggacgt
     1921 cgactttatc agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca
     1981 gtgtgccgcc gttgaaatcg ataaggcgca gatccagttc ttccgcatga cccgccagga
     2041 agcgcaccag tagtttgttg gcattaccca ggccaacgtg gatttcaccg ccggagtgac
     2101 cgcctttcag accttttaag gttaacttga aggtttcaaa accagctgga accgcttcac
     2161 gatctaaatg caggttggag gtgaagtcga taccccccgc acaacccatg tagatttcac
     2221 cttcttcttc ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc
     2281 caaaagcacc gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcggaccgt
     2341 gaaccacgtt ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg ccgttatccg
     2401 cacccagcgt ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat
     2461 ctttcgtgaa gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg aggtgggcct
     2521 gtaagacgac cggtttacga ttttccatac ctgcggtagc aggtttacga atcaggatat
     2581 tacctacctg atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt
     2641 cagcgagttg ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat
     2701 cccacagcgg ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc
     2761 acccgcaaaa cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag
     2821 gcttgcgaga tatgtttgag aataccactt tatctcgcgt cagggagagg cagtgcgtaa
     2881 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
     2941 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
     3001 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
     3061 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
     3121 gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
     3181 tccagctacg atcacgacaa ccagcgagag cttaaagtgc tgaaacgcgc agaaggcgat
     3241 ggcgaaggct tcatagttat tgatgacctg gtggataccg gtggtactgc tgttgcgatt
     3301 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
     3361 ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat
     3421 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcactg
     3481 ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa gtattctgga
     3541 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
     3601 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
     3661 ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
     3721 ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga
     3781 tgctgaacgt accgacgatg atttatacga tactgtgatt ggctaccgtg gtggcaactg
     3841 gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgtg cggaagaaga
     3901 tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta
     3961 tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta
     4021 tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg
     4081 cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt tcccgacagt
     4141 attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta
     4201 ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc
     4261 aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc
     4321 taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa
     4381 cgttgccgtg cgtctggcat atcttgaatc gccgcgtctg aaagcggttg cctgtcttgg
     4441 tccggtagtt cataccctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta
     4501 tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag cgttacgcgt
     4561 ggagctgaat cgctattcat taaaagtgca aggattgctg ggacgtcgct gcccaacgcc
     4621 aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat
     4681 cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa
     4741 ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcgtgt gttaaaaatt
     4801 tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag caatgacgtt
     4861 accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat
     4921 tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa
     4981 cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc tggaagcgca
     5041 ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag gcgactggaa
     5101 gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt
     5161 tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcggatga
     5221 ttttcgtgac gagccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc
     5281 cggcaatatc tattaaaact gatacgtcat cccaatcgcg acaatatcat cattattaat
     5341 attcaatttg ttatcgctat ccagttggtt gattttataa tcaacaaatg ctgacatatt
     5401 tttgttgaaa taatacgtag caccgacgtc gatataatta accagatctt caccaccgat
     5461 gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca gaccaaagtc
     5521 aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc cgccagttat
     5581 tggcgtcatt ttgcgagttt cagaatagaa agttgccaga taaatattat tggcatcgta
     5641 tttcagacct gttgcccatg cttctgcacg cttgccagtg ccacggcttt gcaggttctg
     5701 ctcgttggtg cgatctgagt tggtataggc accactaatg gcgaaatcgc tgccgccaaa
     5761 gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt cgcggttttc
     5821 gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga agaagtcggt
     5881 gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg tctgcgccga
     5941 ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat acaacgcgcc
     6001 caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg caaaagcgag
     6061 acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact ccgcttccca
     6121 ccgaccataa ccagtcagtt gatcgttaat ttgtgtttca cctttaaaac caaaacggat
     6181 ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg ctttaacttt
     6241 gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc
     6301 agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga
     6361 ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa
     6421 cctgagattg ttaaagatat attacagatt aataatattc ttaaaatgtg gtaatttatt
     6481 aaatctgtaa taaaagcgta aacaactgcc gctaggcttg ctgatcccgc gcaacaaaac
     6541 gccatgcttt gctcgcagat ggttggcaac cgacgacagt cctgctaaaa cgttcgtttg
     6601 atatcatttt tcctaaaatt gaatggcaga gaatcatgag tgacagccag acgctggtgg
     6661 taaaactcgg caccagtgtg ctaacaggcg gatcgcgccg tctgaaccgt gcccatatcg
     6721 ttgaacttgt tcgccagtgc gcgcagttac atgccgccgg gcatcggatt gttattgtga
     6781 cgtcgggcgc gatcgccgcc ggacgtgagc acctgggtta cccggaactg ccagcgacta
     6841 tcgcctcgaa acaactgctg gcagcggtag ggcagagtcg actgattcaa ctgtgggaac
     6901 agctgttttc gatttatggc attcacgtcg ggcaaatgct gctgacccgt gctgatatgg
     6961 aagaccgtga acgcttcctg aacgcccgcg acaccctgcg tgcgttgctc gataacaata
     7021 tcgttccggt aatcaatgag aacgatgctg tcgctacggc agagattaaa gtcggcgata
     7081 acgataacct ttctgcgctg gcggcgattc tggcgggtgc cgataaactg ttgctgctga
     7141 ccgatcaaaa aggtttgtat accgctgacc cgcgcagcaa tccgcaggca gaactgatta
     7201 aagatgttta cggcattgat gacgcactgc gcgcgattgc cggtgacagc gtttcaggcc
     7261 tcggaactgg cggcatgagt accaaattgc aggccgctga cgtggcttgc cgtgcgggta
     7321 tcgacaccat tattgccgcg ggcagcaagc cgggcgttat tggtgatgtg atggaaggca
     7381 tttccgtcgg tacgctgttc catgcccagg cgactccgct tgaaaaccgt aaacgctgga
     7441 ttttcggtgc gccgccggcg ggtgaaatca cggtagatga aggggcaact gccgccattc
     7501 ttgaacgcgg cagctccctg ttgccgaaag gcattaaaag cgtgactggc aatttctcgc
     7561 gtggtgaagt catccgcatt tgcaacctcg aaggtcgcga tatcgcccac ggcgtcagtc
     7621 gttacaacag cgatgcatta cgccgtattg ccgggcacca ctcgcaagaa attgatgcaa
     7681 tactgggata tgaatacggc ccggttgccg ttcaccgtga tgacatgatt acccgttaag
     7741 gagcaggctg atgctggaac aaatgggcat tgccgcgaag caagcctcgt ataaattagc
     7801 gcaactctcc agccgcgaaa aaaatcgcgt gctggaaaaa atcgccgatg aactggaagc
     7861 acaaagcgaa atcatcctca acgctaacgc ccaggatgtt gctgacgcgc gtgccaatgg
     7921 ccttggcgaa gcgatgcttg accgtctggc actgacgccc gcacggctga aaggcattgc
     7981 cgatgatgtg cgccaggtgt gtaacctcgc cgatccggtg gggcaggtaa tcgatggcag
     8041 cgtactggac agcggcctgc gtcttgagcg tcgtcgcgta ccgctggggg ttattggcgt
     8101 gatttatgaa gcgcgcccga acgtgacggt tgatgtcgct tcgctgtgcc tgaaaaccgg
     8161 taatgcggtg atcctgcgcg gtggcaaaga aacgtgtcgc actaacgctg caacggtggc
     8221 ggtgattcag gacgccctga aatcctgcgg cttaccggcg ggtgccgtgc aggcgattga
     8281 taatcctgac cgtgcgctgg tcagtgaaat gctgcgtatg gataaataca tcgacatgct
     8341 gatcccgcgt ggtggcgctg gtttgcataa actgtgccgt gaacagtcga caatcccggt
     8401 gatcacaggt ggtataggcg tatgccatat ttacgttgat gaaagtgtag agatcgctga
     8461 agcattaaaa gtgatcgtca acgcgaaaac tcagcgtccg agcacatgta atacggttga
     8521 aacgttgctg gtgaataaaa acatcgccga tagcttcctg cccgcattaa gcaaacaaat
     8581 ggcggaaagc ggcgtgacat tacacgcaga tgcagctgca ctggcgcagt tgcaggcagg
     8641 ccctgcgaag gtggttgctg ttaaagccga agagtatgac gatgagtttc tgtcattaga
     8701 tttgaacgtc aaaatcgtca gcgatcttga cgatgccatc gcccatattc gtgaacacgg
     8761 cacacaacac tccgatgcga tcctgacccg cgatatgcgc aacgcccagc gttttgttaa
     8821 cgaagtggat tcgtccgctg tttacgttaa cgcctctacg cgttttaccg acggcggcca
     8881 gtttggtctg ggtgcggaag tggcggtaag cacacaaaaa ctccacgcgc gtggcccaat
     8941 ggggctggaa gcactgacca cttacaagtg gatcggcatt ggtgattaca ccattcgtgc
     9001 gtaaataaaa ccgggtgatg caaaagtagc catttgattc acaaggccat tgacgcatcg
     9061 cccggttagt tttaaccttg tccaccgtga ttcacgttcg tgaacatgtc ctttcagggc
     9121 cgatatagct cagttggtag agcagcgcat tcgtaatgcg aaggtcgtag gttcgactcc
     9181 tattatcggc accatcaaga aaatcaataa cttagcttaa cttctctaag aaattgtatc
     9241 ttctgttatc acttacaaac gcctatacgt tgttatgggc tttggtagtg taacacgtat
     9301 gggcgagtag ttcccaggct caacctcgta acaagttcct ttgaagctgg aagacgtgcc
     9361 accgtctcta aaccttctac caaaactctt agcaagatgt tccacacctg gcgcggtgtc
     9421 caatgatgct acacgtcgtg agacgttgca gaggataaac aggctaaatg atgggaagag
     9481 ttttaagtgc aatagtggat taaagcccac ctcttcaaaa cctaacttga tcgggaaaga
     9541 atcaagctct agcacggttt cgtccaccgg agtacaacgg gtgcctgata agagaagaac
     9601 tgctaagact tcatagcttt tgtgaaaaag ccctttttag tggatacgcc tacggcgctt
     9661 attttttggt ataaccatca aaggcgcttt atggctccta ttgtcttgaa aatgtatctt
     9721 tacttgtatt gtgttttatg cgtagtctcc ctgtgtagta tgtaacttgt taattttcat
     9781 atgaatacag aggcaacacg atgaaaaaca ttgcagctat aaaacgtaat aaccgcaaga
     9841 ttcacgctcg taagttcctg tctacgccag aaggaaaagc ctggttagag cgtaaacaga
     9901 gagagaacga agaaagaaaa ctccttagtg agttgaaatg gcttagagag gatttctgaa
     9961 aatctccaga acgcaacagg acgcattcta acgctgtttt cattgcttgg tatagggatg
    10021 tataggtgat gtgtgtttaa tgcgtctgtg cgcttgtttt gtgccttatt ttgcattttc
    10081 ataatgcaga agagaacacg gaaaacctta aaattaatta ttggggtatt taaggc
//
LOCUS       DAZKIV010000157         9993 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZKIV010000157 DAZKIV010000000
VERSION     DAZKIV010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.157"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..153)
                     /locus_tag="ACHOT6_003939"
     CDS             complement(<1..153)
                     /locus_tag="ACHOT6_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949086.1"
                     /translation="MLQSSADKACNVMVKKVRYHKDVREITKMMNGKLIYSWLKWPCG
                     EFFLLVV"
     gene            231..1325
                     /gene="ddlA"
                     /locus_tag="ACHOT6_003940"
     CDS             231..1325
                     /gene="ddlA"
                     /locus_tag="ACHOT6_003940"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFP9949087.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1349..1561)
                     /gene="yaiZ"
                     /locus_tag="ACHOT6_003941"
     CDS             complement(1349..1561)
                     /gene="yaiZ"
                     /locus_tag="ACHOT6_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFP9949088.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1821..2129
                     /gene="yaiY"
                     /locus_tag="ACHOT6_003942"
     CDS             1821..2129
                     /gene="yaiY"
                     /locus_tag="ACHOT6_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFP9949089.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2188..3282)
                     /gene="yaiW"
                     /locus_tag="ACHOT6_003943"
     CDS             complement(2188..3282)
                     /gene="yaiW"
                     /locus_tag="ACHOT6_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFP9949090.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3295..4515)
                     /gene="sbmA"
                     /locus_tag="ACHOT6_003944"
     CDS             complement(3295..4515)
                     /gene="sbmA"
                     /locus_tag="ACHOT6_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFP9949091.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4867..6024
                     /gene="ampH"
                     /locus_tag="ACHOT6_003945"
     CDS             4867..6024
                     /gene="ampH"
                     /locus_tag="ACHOT6_003945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFP9949092.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6025..6652)
                     /gene="iprA"
                     /locus_tag="ACHOT6_003946"
                     /pseudo
     CDS             complement(6025..6652)
                     /gene="iprA"
                     /locus_tag="ACHOT6_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
     gene            complement(6740..9742)
                     /gene="ehaB"
                     /locus_tag="ACHOT6_003947"
     CDS             complement(6740..9742)
                     /gene="ehaB"
                     /locus_tag="ACHOT6_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFP9949093.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTI
                     ASWDDVALAVVAHPNADNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDADG
                     EVDTNGWDGTDRLDLKLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATAT
                     LIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTGSQWDTTKTSL
                     IDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEV
                     SAGWAVGDAALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNI
                     ESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEV
                     DNNGDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAY
                     TYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNG
                     GAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDR
                     SGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWG
                     FGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAG
                     YTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNF
                     SAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2577 a         2474 c         2373 g         2569 t
ORIGIN      
        1 tacaactaag agaaaaaact caccgcatgg ccatttaagc caggaataaa ttagcttccc
       61 gttcatcatt tttgtgatct cgcgcacatc tttgtgataa cgcactttct tcaccattac
      121 gttgcacgct ttgtcagcag aagattgcaa cattctgtca gaaggccgcg aatcccccgt
      181 tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac
      241 tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg
      301 caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg
      361 ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc
      421 ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg
      481 agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc
      541 cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca
      601 atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca
      661 ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg
      721 ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg
      781 taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt
      841 acgcaattgc cgtcgatctg gcgttcgaat tcgatcataa agtgatcgtt gagcaaggga
      901 tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caacccgcaa gccagcacct
      961 gtggcgagat cgtactcacc agcgacttct atgcctacga caccaagtac attgacgaag
     1021 atggcgcgaa agtggtagtt ccggcagcca ttgcgccaga aatcaacgat aagatccggg
     1081 cgattgccgt tcaggcttat caaacgttgg gatgcgcagg catggcgcgt gtagacgtgt
     1141 ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca
     1201 acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca
     1261 cgcgtttgat tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa
     1321 tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac
     1381 gacgaataat cagccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat
     1441 aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta agaatgaata
     1501 taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca
     1561 tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg
     1621 tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt tgtgactcac
     1681 aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga
     1741 caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca
     1801 gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa
     1861 aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt
     1921 gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc
     1981 gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca
     2041 ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat
     2101 ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc
     2161 cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc
     2221 tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg
     2281 aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata
     2341 aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg
     2401 gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt
     2461 tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc
     2521 gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt
     2581 aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta
     2641 agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat
     2701 tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc
     2761 accggtgcgc accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat
     2821 cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct
     2881 ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat
     2941 gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac
     3001 ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt
     3061 ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc
     3121 atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc
     3181 tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac
     3241 cgccaacaat gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct
     3301 caaggtatgg gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag
     3361 acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa
     3421 agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt
     3481 accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata
     3541 gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg
     3601 tacggcgcta aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc
     3661 ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg
     3721 cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc
     3781 aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat gcgcggagag
     3841 cgttaccagc accggcagaa aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc
     3901 catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcccc
     3961 ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca
     4021 gcggaacaca tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag
     4081 cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg
     4141 cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac
     4201 cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca
     4261 gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac
     4321 aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg
     4381 gatctgcccg gaagcgccgg tgatacgcgc cacccagtca cccccaccgg cttgccagaa
     4441 gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa
     4501 aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat
     4561 taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca
     4621 aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag
     4681 attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg
     4741 aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct
     4801 ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca
     4861 ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc
     4921 gtccatgcgg cgcagccgat cactgaaccg gagttcgcct ctgatattgt tgatcgttat
     4981 gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt
     5041 aaccagcgcg tctttcgcag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag
     5101 ctggattcgg tcgtgcgtat tgcttccctc accaagctaa tgaccagtga gatgctggtg
     5161 aaattgctcg accaggggac cgtgaagtta aacgatccgc taagtaaata tgctccgcca
     5221 ggcgcacgcg tgccaaccta caatgggacg cccattacgc tggtcaatct ggcaacccat
     5281 accagcgcgc tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg
     5341 ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc cgcgccaggc
     5401 agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg ctgatgcact ggcgaatgcg
     5461 tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa
     5521 gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc agagcgcggt
     5581 gccagtccat gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct
     5641 ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac
     5701 caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa agtgattggc
     5761 atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa
     5821 gagggtcgtc cggggattat tcagaagaca ggaggtggtg gcggtttcat tacctatatg
     5881 gcgatgatcc cgcagaagaa tattggcgcg tttgtggttg tcactcgttc gccgttgacg
     5941 cgctttaaaa atatgagcga tggcatcaat gacctggtga ccgagctaag cgggaataaa
     6001 ccgctggtta tccccgcgtc ctgattaata ctctgaaggc aaacggttga tagcgaccag
     6061 tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac
     6121 gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgagcg
     6181 caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt
     6241 atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta
     6301 aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt tgtttggctg
     6361 gtagatgata tcccgtacaa tttccttctg atattaattt gtatggtata tcgtttttca
     6421 ttaaaccatc agccagcccc ataatataag gagcctgggt aataccgata agtacgtttt
     6481 cttctctacg cagagagata acgccctcta aaataacgaa agtatcttcg ctattgacat
     6541 cactggaaaa tataacttgc ttttcattat taaactcgaa gcgcgtaccg tatctggaca
     6601 aacatttatc gagcttacca aattcctgaa gaggtttaac tacagataac atttgcgcgt
     6661 cctttgcagt aatgcccgtc aaatccttga cgggcattat ttagattaaa ttaccagaat
     6721 ttcttcggag tgaagaatat taccaggtat atttaacacc cacgttcgcg gaccagtctt
     6781 gatctacgtc accaccaccg aggtagttag catcggtata ggcgctgaag ttcttggtga
     6841 agctgaactg agtacccaga ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg
     6901 aatcaccgtt cacatcgtta tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt
     6961 acggagtcag agcctggtct tcgctgtagg tgaaggtata acctgcatct acacccagtt
     7021 cataacgcat gctgtcgtaa gactgaccgt caactttcat gtcgttgctc agctggtagt
     7081 catcaccaga ctggaacaga ccagaaacgc tgccgtaagg agtcacgtaa ccagcatcac
     7141 ccagtttgaa gtcgtaaccg gctttcaaac cgaagcccca cgcgtcagag ttggtgctac
     7201 cgtcaacgta agtaccgttg ctcatggttg cagacaggtc gttgttgaag tgagagtagc
     7261 tcaagctacc atcaacaaag acgttgttcg cgaagtgagc agaagagtag atgtaggcag
     7321 tctggctgtc ttgatccacc tgaccagaac ggtcattcat gtcaccttta gcgaagcctg
     7381 cagccgcacc gacgatccac ttagcgttgt taccgtcaat tttggtatca acaccgacca
     7441 tgatgccgtt aacatcctga tcatagttga tggtgccgtt gtcgccgttg aagttaccac
     7501 cgaagtagct tacccatgcg ccgccgttat cagccaggcc atgacgagag ttggtcagac
     7561 gagtaccaac ggtgtcttgt tccaggttcc agatattggt gttcgcagac gggatgctca
     7621 gcgccatgtt agcgtagtcg gtcagctcca tctgttgcag aacaacggtg ttaccgcgct
     7681 gttcagcctg ataggtgtat gcacccaggt cagctttgtt agcagcagag aaggtcgcgt
     7741 tgctgttttt gtcgttgacg tagatcagct cgttgccgtt gtagtcagcg atagaaccag
     7801 caccagttgc gttgtcgata cgaactttgt agttaccggt tgcagctgca acatggtcac
     7861 cgttgttatc aacttcgttc tgaccagctt cgatagaagc agtgttgtcg ttatcgccgt
     7921 taccgttaat ggtcaggtga ccatcagagt tcattgcgat taagccataa ccgtagtttg
     7981 acttggtagt atcgttggta cggtcgttgg tcaggtctgc atcaagtacg aagcggttgc
     8041 tttcgatgtt gaacacacca gcgtgaatgt taccactggt atcagtagtg ctggtcaggt
     8101 tcagagtatc aacctgcaga gcattcgccg cagtgttacc tacatccaga ataccgtcgt
     8161 tagtcacttt gatgttgttg gcatacagag cagcatcacc tacagcccaa ccagcagaaa
     8221 cttcatcaga aatggttacg gtactgttgt cgacagtcag tgaatcagtt gcaacatgac
     8281 catcttcgtt gatgttcagc gcagaaagac ctgtcagaga gatagagtca gaaatcagcg
     8341 ttgaatcagc aacattaaca gttgaaccac tgttgatgct cagagtatca atcagagaag
     8401 ttttggtggt atcccactgt gaaccggtat tcagggtcac attgaacaga ccgctctggt
     8461 aaacctgatt accagtaata tgatcgaatt cactgtaact tgtatcatcg ttttcaacac
     8521 caacggttga taacggccac aggctattat cctcaatatc aatcagagtt gcagttgcct
     8581 cggtgccaac accgtatgta gcgatgtcac ctttaccatc tccatcaaca tcaatagaac
     8641 cagtttgatg aacagattgt gctgccccta cccatttact accgttattt aatttcagat
     8701 ccaggcgatc tgttccatcc caaccgttgg tgtctacttc accatcagca tcgcgataac
     8761 tatctgcacc acgtgggaag aagttttcat caaagttgct agagaagatt acatcgccca
     8821 tcaaagtaga gttactaaag tcagcggtgg tctgcatagc attatcagca ttcggatgtg
     8881 caactacagc taatgcaaca tcatcccagc ttgcaatagt gctatcagca acaccatctc
     8941 cgtcagtatc tacggtgaca aagttagatt taccactata atcgctggca ttacctgtat
     9001 taccgaacca accagtagta ccttcatcag tccatgaacc agaggttacg gtagaatctt
     9061 taacagtgat ggtgttatta aacacttcgc tgctgtttac gccagtgctg acagtgttgt
     9121 cgtcaatatc ttcccactca taaccctgag tcaacgtaat acccgcaacg tgagagttat
     9181 tagtgatgga cagatcaact tcctgatcca gagtaatagc cgtaccaatg ttgtaaacgt
     9241 ctacaacatg agtatcagcg gcattattgt aagtgccgtt atatgtgtag tgttcgtagt
     9301 tgtcgtcgat cgttgaatta tcaacagtca gcgccagacg gtcatggtag taatcatccg
     9361 cacgatcagc acaatcagtt gtcatgcact cggaataaat cataccgtga atagtgctgt
     9421 ttttgattgt cagactattg gcgttggtat tggtagaaat accgtcgtcc agatagtagg
     9481 tagaaatcac gccgttaacg gtagcgttat taatgaccgg gtagatatcg ccgttgtaat
     9541 agctatcagc ggcatagttg ttccagccta cataaccatc atagtaaacg tcatcagcat
     9601 aggtggcgtc gttgtagtga tggaaagtat tgtaagtttg accagaaata tcgttggtcg
     9661 ctgcattagc ctgagaggtg atagccagag tgcaagccaa tgctaattgt gatactacaa
     9721 gtttcttttt ccaggagtgc atttgtcatc cctcctcagg gacgtaataa gttgatccat
     9781 caattatcaa tgcatagaaa acatattatg tttcgcgcta acgattatgc tgcgttacag
     9841 acgaatggtt caattattct ttcccctaag tccgaatgtg gacatatata atttatggag
     9901 aaaaacacgc atcaaattgg taaaaggacg atatttcaac atgttgcaga taatgttatt
     9961 tttgatacat acgcgttata aatatcaaaa aca
//
LOCUS       DAZKIV010000158         9916 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZKIV010000158 DAZKIV010000000
VERSION     DAZKIV010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9916
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.158"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            51..623
                     /gene="lpfA-O113"
                     /locus_tag="ACHOT6_003948"
     CDS             51..623
                     /gene="lpfA-O113"
                     /locus_tag="ACHOT6_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HFP9949094.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            671..1402
                     /gene="lpfB-O113"
                     /locus_tag="ACHOT6_003949"
     CDS             671..1402
                     /gene="lpfB-O113"
                     /locus_tag="ACHOT6_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_316045823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial chaperone LpfB-O113"
                     /protein_id="HFP9949095.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1427..3949
                     /gene="lpfC-O113"
                     /locus_tag="ACHOT6_003950"
     CDS             1427..3949
                     /gene="lpfC-O113"
                     /locus_tag="ACHOT6_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial usher protein LpfC-O113"
                     /protein_id="HFP9949096.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGQTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKRFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAAGLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSLSTPESESGLIEQGVTCH"
     gene            3960..5033
                     /gene="lpfD-O113"
                     /locus_tag="ACHOT6_003951"
     CDS             3960..5033
                     /gene="lpfD-O113"
                     /locus_tag="ACHOT6_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="LpfA-O113 is the major subunit of a form of long
                     polar fimbria first described in LEE-negative, Shiga
                     toxin-producing enterohemorrhagic Escherichia coli (STEC)
                     of serotype O113:H21. This protein, designated LpfD-O113,
                     is the minor subunit of this chaperone-usher type fimbrial
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial minor subunit LpfD-O113"
                     /protein_id="HFP9949097.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKMVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            5281..6321
                     /gene="pstS"
                     /locus_tag="ACHOT6_003952"
     CDS             5281..6321
                     /gene="pstS"
                     /locus_tag="ACHOT6_003952"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFP9949098.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVDQVRAAWK
                     TNIKDSSGKPLY"
     gene            6408..7367
                     /gene="pstC"
                     /locus_tag="ACHOT6_003953"
     CDS             6408..7367
                     /gene="pstC"
                     /locus_tag="ACHOT6_003953"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFP9949099.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            7367..8257
                     /gene="pstA"
                     /locus_tag="ACHOT6_003954"
     CDS             7367..8257
                     /gene="pstA"
                     /locus_tag="ACHOT6_003954"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFP9949100.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            8348..9121
                     /gene="pstB"
                     /locus_tag="ACHOT6_003955"
     CDS             8348..9121
                     /gene="pstB"
                     /locus_tag="ACHOT6_003955"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFP9949101.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            9136..9861
                     /gene="phoU"
                     /locus_tag="ACHOT6_003956"
     CDS             9136..9861
                     /gene="phoU"
                     /locus_tag="ACHOT6_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFP9949102.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         2547 a         2274 c         2528 g         2567 t
ORIGIN      
        1 atataagtga tgattcatgg taaaggatat attatatcaa tggagttttt atgaagcgta
       61 atattatagg cggtgcattc actctggcat ctctaatgct ggccgggcat gcactggcag
      121 aagatggtgt tgttcacttc gtcggtgaaa ttgtcgacac tacttgtgaa gttacctccg
      181 atacagccga tcaaattgtc ccactgggta aagtcagtaa aaatgcattt tcaggtgtag
      241 gtagtctggc gtcgccacag aagttcagta ttaaacttga aaattgcccg gcaacgtaca
      301 ctcaagcagc cgttcgtttt gatggtacag aagcgcctgg cggcgacggc gacctgaaag
      361 tgggtacgcc gcttacagca ggcaaccctg gtgattttac cggtacagga caagcgattg
      421 cggcaaccgg cgttggtatt cgtattttta accagtccga taattcgcag gttaaacttt
      481 ataacgactc tgcttatacc gctatcgatg ctgaaggcaa ggctgaaatg aagtttattg
      541 cacgctatgt ggcaaccaat gcgaccgtaa cggctggtac ggcgaacgcg gattcacaat
      601 ttactgtcga atataagaaa taatttatca agggcctttt tattcagttg gctttggcca
      661 gggagtcgtt atgtccagaa aaagttgttt aatcaggctc tttaaaacaa cagtttcatg
      721 taagttattt accgcgatta tactcagcgt ttttttaggc caaccagcat taacttatgc
      781 aggcgttgtc attggtggta cgcgggtggt ttacttaagt aataatgctg ataaatctat
      841 ttctgtattt agcaaagaag aaaaaatacc ttatctcatc caggcttggg tagatccatt
      901 taataaagag gataaaagca aagcgccgtt taccgttatt ccgccagtat cacgattaga
      961 accttcgcaa gaaaaaatat tacgcattat tcacaccaaa ggtgtgtcat tgcctgatga
     1021 tcgcgagtca gtcttctggc tgaatattaa aaatattccg ccatcagcga gcaacaaagc
     1081 gacgaactcg ctggaaatag cagtaaaaac gcgtatcaag ctgttctggc gaccagcaaa
     1141 tatccgcctt attcccgaag atgctgcgcc gaaagtgaaa tggcgccggg aagggcgaaa
     1201 tctgatagca gagaatccca acccaattca tatcagtgtg atggatgtca tcgttgatgg
     1261 ccatgatgtg cccctcaata tgatccgtcc ttttgaaacg ctcacgttac ctttacctgc
     1321 taattctgcg ggaggccaaa tgacctggcg atttattaat gattacggcg cagtcagtga
     1381 cccgataaaa atgacgctgt aagcaaggga taaggatagg cgtattatga tgacgaccag
     1441 aatagtggtt ggcctcacgg cagggacgtg tctgattttc tcgcaaaacc tgatggccga
     1501 ggtcagtgta ttcaatccgg cgcttctgga aatcgaccat caatccggag tcgatattcg
     1561 ccagtttaat cgggcaaacc tgatgccccc aggtgtttat agcgttgata tttttatcaa
     1621 cggtaaaatg tttgaacgtc aggatgtgac atttgttcag gataatccag atgctgatct
     1681 gcacgcttgc tttattgcca ttaaaaaaac actgtcctcc tttggcataa aagttgatgc
     1741 gctcaaatcg ttcaatgatg tggatgagac ggtttgcctc gatcctgctc cacgtattga
     1801 aggctcatcc tggcagtttg acagtgataa attgcagctg aatatatcca ttccccaaat
     1861 ctacatggac gcgatggctt atgattacat cagccccacg cgttgggatg aggggattaa
     1921 cgcgctcacc atcaactacg atttttctgg ttcacataca ctacgttcag attatggttc
     1981 acaagagaca gataccagtt atctcaatct gcgcaatgga ctgaatattg gaccgtggcg
     2041 gctacgtaat tacagtactt taaacaccag cgatggccgt gcggaataca actccattag
     2101 tacctggata cagcgcgata ttgccgcgtt aagaagccag attatgattg gtgatacgtg
     2161 gacggcgagc gatattttcg acagtacgca aattcgcggc gcgcgtttgt atactgataa
     2221 cgatatgcta cccgccagcc agaatggctt tgctcctgtg gttcgtggga ttgcaaagtc
     2281 caacgccacc gtcatcattc ggcagaatgg ctacgtgatt tatcagtctg ccgttccaca
     2341 aggtgctttt gagattaccg atctcaacac cgccagtaca ggtggcgatt tggacgtaac
     2401 catcaaagaa gaagacggta gcgaacaacg attcacccaa ccttatgctt cattggcgat
     2461 tcttaaacgt gaaggtcaga cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga
     2521 cggatttaca ccggacgtcc ttcaggcgca aatacttcat ggtttttccc acgggatcac
     2581 tttatatgga ggtatgcagg ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa
     2641 agatcttggc gctttgggcg caatttcttt cgatgtgaca catgctcgtg cgaattttag
     2701 ccatgatgat acagaaacgg gtcagtcata tcgctttctc tattcaaaac gatttgacga
     2761 cacagacact agcttgcgcc tggttggcta tcgttactcc accgagggct actataccct
     2821 caatgagtgg gcatcgcggc gcaacagccc tgaagacttt tgggaaacag gtaaccgacg
     2881 tagtcgcgtg gagggaacgc taacgcagtc gttggggaga gattatggca atttatacct
     2941 gacattaagc cggcaacaat actggcatac cgatgatgtc gaacgattaa tgcaatttgg
     3001 ctacagcagt agctggaagc gtctctcgtg gaacgtctcc tggagttatt ccaatactgc
     3061 cagacagggg acggggaaca accatgccag tgataacacc agtgagcaga tctacatgct
     3121 ctctttatct gttcctttat cgggctggtg gggtaatagt tacgccacct attctgtttc
     3181 gcaaaacgat aattccggta gctcacatca actcggactc agcggtacgg cgctggaaag
     3241 aaataacctt tcatggaatt taatgcagtc ctataacagt catgatgatg aggttggcgg
     3301 taatatgtcc ctgacctatg atggctctta tggcacggtg aacggcagct ataactacag
     3361 ccaaaattcc cagaggctga attatggtat cagaggggga attctggcac acagcgaagg
     3421 ggtaacgtta agtcaggagt taggtgaaac tattgctctt gttaaagcac ctggggccgc
     3481 cgggttagaa atagataata tgcgcggtgc tgcgacggac tggcgcggct atacggtcaa
     3541 gacacagcta aacccttatg atgaaaatcg ggtagcaatc agcgataact atttctcgaa
     3601 gtcgaatata gaacttgata ataccgtcgt tacgatggtt cccacgcgtg gtgcagtggt
     3661 taaagcggag tttgtgactc atgtgggtta tcgcgttctc ttcagagtgt taaatgcaaa
     3721 tggtaaaccg gtaccttttg gagccattgc tgcgatacaa gatgcaagtt tggcagattc
     3781 aggaattgtc ggtgaccgtg gcgaacttta tctttctggt ctaccagaaa aaggacaggt
     3841 tacgttatcc tggggagaaa acgcctcaac aaaatgcatc ttcaattatt cactttcgac
     3901 accagaaagt gagagcggat taattgaaca gggtgtgaca tgtcattaaa aggattttta
     3961 tgaacaagta tatcaaacag tggtgctttg ctgtgtttat gctctcgtta agtagtgtag
     4021 cacttgcagc tcctaaaggt atctgtaccc ctgataatgg cgtatttcac agtacgcttg
     4081 atttctcggg atatcttatt accgcgaacg aaaataaggt tggaacgaca tttaacacca
     4141 ctgtaactaa tggatcctca tatcctggac gctgtcattg tgatactggt aacgtaggag
     4201 aattccccta tatctattat acgtcaaaaa taaaccaggc attaacttat gctggggttc
     4261 actctaatat taattattat gatcttaatc cgaaccttga tgttgggatt gctatagata
     4321 ttcttggggt tgggtatgtg aatgcacctt ttgaatatca tgctaacaac ccgtctggta
     4381 atacaaagta caattgcaac cgcattgaac ctttaagtat atctagtggg gctaaagcga
     4441 tagtatattt ttatatcaag aagacatttg caggaaaatt gattattcct gaaacaaaga
     4501 tagtgacatt gtatggaaca attagccgtg acaccccggt ggattactca cagccgatgg
     4561 ctgatgttta tattcggggc gatattacag ctccgcaaag ttgcgaaata aataatttac
     4621 agccagttta ttttgatttt aaagaaatac cagctgcaga tttttcatct gttgttggaa
     4681 gtgcggtaac aacacataaa attactaaaa tggtcactat tgagtgtgaa aacctgggga
     4741 tactgaatac cgatgatatc agtacgtctt tttatgctac cgaacccaat acggacaatt
     4801 caatggtcgt aacttcaaac tctaacgtgg ggataaaaat ttacgacaaa aataataagg
     4861 aaatcaaagt gaacggtggt gagttgccaa cagacatggg taaatcaaca gtgtatggtg
     4921 aaaaatcagg tagtgtaact ttttcagctg ctcctgcgag ccttactgga gctcgcccag
     4981 ccccggggca atttactgca acagcgacga taactgttga gattgtgcgc taataggtct
     5041 gacaacgatt gttcatcaca tcgttgtcat ctcacccatt tctttatcac ttttaaatgc
     5101 atcatgacaa aaagatgaca tgtgggattt tcatcttcac atttaaaatc agtaagttat
     5161 ttggtaattc ctttctgtca taaaaatgtc atattcctta catataactg tcacctgttt
     5221 gtcctatttt gcttctcgta gccaacaaac aatgctttat gaatcctccc aggagacatt
     5281 atgaaagtta tgcgtaccac cgtcgcaact gttgtcgccg cgaccttatc gatgagcgct
     5341 ttctctgtgt ttgcagaagc aagcctgaca ggtgcaggtg caaccttccc tgcgccggtg
     5401 tatgccaaat gggctgacac ttaccagaaa gaaaccggta ataaagttaa ctaccagggt
     5461 atcggttctt ccggtggcgt aaaacagatt atcgctaata ccgttgattt tggtgcctct
     5521 gacgcgccgc tgtctgatga aaaattggct caggaaggcc tgttccagtt cccgaccgtg
     5581 attggcggcg tggtgctggc tgttaacatt ccagggctga agtctggcga actggtgctg
     5641 gatggtaaaa ccctcggcga catctacctg ggcaaaatca agaagtggga tgatgaagcc
     5701 atcgccaaac tgaatccggg tctgaaactg ccttcacaga acattgctgt agtacgccgc
     5761 gcagatggct ccgggacttc cttcgtcttc accagctacc tggcgaaagt gaacgaagag
     5821 tggaaaaaca acgttggtac tggctctacc gtaaaatggc cgatcggtct gggcggtaaa
     5881 ggtaacgacg gtatcgccgc gttcgttcag cgtctgccgg gtgcaattgg ttacgttgaa
     5941 tatgcttacg cgaagcagaa caacctggcg tataccaaat tgatctccgc tgatggtaaa
     6001 ccggttagtc cgaccgaaga aaacttcgct aatgcagcaa aaggcgcaga ctggagcaaa
     6061 accttcgctc aggatctgac caaccagaaa ggcgaagatg cgtggcctat tacctctacc
     6121 acgttcattc tgatccacaa agatcagaag aaaccagaac aaggcacaga agtgctgaaa
     6181 ttcttcgact gggcgtacaa aaccggggct aaacaggcga acgacctgga ttacgccagc
     6241 ctgccggata gcgtagttga ccaggttcgc gctgcgtgga agaccaatat taaagacagt
     6301 agcggtaagc cgctgtacta ataaaactcc aggccgggta cggtgtttta caccgcatcc
     6361 ggcattacaa aataactttg taaacgcgtt taactgaaga gtaacttatg gctgcaacca
     6421 agcctgcttt taacccaccg ggtaaaaagg gcgacataat tttcagcgtg ctggtaaaac
     6481 tggcggcgct gattgtgcta ttgatgttgg gtggcattat tgtctctctg atcatctcct
     6541 cctggccgag cattcagaaa tttggtctgg cttttctgtg gaccaaagag tgggatgcac
     6601 cgaacgatat ctacggggcg ctggtgccga tctacggtac gctggtgact tcgtttatcg
     6661 cgctgctgat cgccgtcccg gtgagtttcg gtatcgccct gttcctgact gagcttgcgc
     6721 ctggctggct gaaacgcccg ctgggtatcg ccattgagct gctggcagcc attccaagta
     6781 tcgtttacgg catgtggggc ctgtttatct ttgcgccgct gttcgccgtt tacttccagg
     6841 agccggtcgg caatatcatg tcgaatatcc cgattgttgg cgcgctgttc tctggccccg
     6901 catttggtat cggtatcctc gcggcaggcg tgatcctcgc catcatgatt attccgtaca
     6961 ttgcggcggt aatgcgtgat gtgttcgaac aaaccccggt gatgatgaaa gagtcggcct
     7021 acggtattgg ctgcaccacc tgggaagtta tctggcgtat cgttcttccg ttcaccaaaa
     7081 atggtgttat cggcggcatc atgctggggc tgggccgcgc gctcggtgaa accatggcgg
     7141 tgacctttat catcggtaac acctaccagc tcgacagcgc ctcgctgtat atgccgggca
     7201 acagtatcac ctctgcgctg gcgaacgaat ttgcggaagc ggaatccggt ctgcacgttg
     7261 ccgcactgat ggaactgggc ctgatcctgt ttgtgattac cttcatcgtc ctcgccgcat
     7321 cgaagtttat gattatgcgc ctggctaaga atgagggggc acgctaatgg ctatggttga
     7381 aatgcaaacc actgcggcgc tggctgaatc tcgccgcaaa atgcaggcgc gtcgccgcct
     7441 caaaaaccgt attgcgctga cgctctcgat ggcgacgatg gccttcggcc tgttctggct
     7501 gatctggatt ttaatgtcca ccatcactcg cggtatcgac ggtatgtcgc tggcgctgtt
     7561 cactgaaatg acgccgccgc ccaatacgga aggtggtggt ctggcgaacg ccctggcggg
     7621 tagcgggctg ttaattttgt gggccacggt attcggtacg ccgctgggca ttatggcggg
     7681 gatttatctg gcggaatatg gtcgtaaatc ctggctggca gaagtgattc gcttcattaa
     7741 cgacattctg ctctccgcgc cgtcgattgt ggttggtctg tttgtttaca ccatcgtggt
     7801 ggcgcagatg gagcacttct ccggctgggc gggcgtgatt gccctggcgc tgttgcaggt
     7861 gccgattgtt atccgcacca ccgagaacat gctgaaactg gtgccggaca gcctgcgtga
     7921 agcggcttat gcgctgggta caccgaagtg gaagatgatc tccgcgatta cgctgaaagc
     7981 gtcggtgtcc gggatcatga ccggtatctt gctggcgatt gcccgtattg ctggtgaaac
     8041 cgcgccgctg ctgtttaccg cgctctccaa ccagttctgg agcacggaca tgatgcagcc
     8101 gatcgccaac ctgccggtga cgatctttaa gtttgcgatg agtccgtttg cggaatggca
     8161 gcaactggcc tgggccgggg tattgatcat taccctgtgc gtactgctgc tgaacattct
     8221 ggcgcgcgtt gtttttgcga agaataaaca cggttaacac tttgccggat gcggcgtgaa
     8281 cgcctgatcc ggcctacggt aagcctgatt cgcgaagtgc atcaggcacg atgaggaaaa
     8341 gattgcaatg agtatggttg aaactgcccc gagtaaaatt caggttcgta atttgaactt
     8401 ctactacggc aaattccatg ccctgaaaaa catcaacctg gatatcgcta aaaaccaggt
     8461 tacggcgttt atcgggccgt ccggctgcgg taaatcgacg ctgctgcgta ccttcaacaa
     8521 aatgtttgaa ctgtacccgg agcagcgtgc ggaaggtgaa attctgcttg atggcgacaa
     8581 catcctgacc aactctcagg atatcgccct gctgcgtgcg aaagtgggca tggtgttcca
     8641 gaaaccgacg ccgttcccga tgtccatcta cgacaacatt gcctttggcg ttcgtctgtt
     8701 tgagaagctc tcccgtgccg acatggacga gcgcgtgcag tgggcattga ccaaagccgc
     8761 attgtggaac gaaaccaaag ataaattgca ccagagcggt tactctctct ctggtggtca
     8821 gcaacagcgt ctgtgcattg cgcgtggtat cgccattcgc ccggaagtgc tgctgctcga
     8881 cgaaccgtgt tcggcgctcg accccatctc taccgggcgt attgaagagc tgatcaccga
     8941 actgaagcag gattacaccg tggtgatcgt cacccacaac atgcagcagg ctgcgcgttg
     9001 ttccgaccac acggcgttta tgtacctggg cgaattgatt gagttcagca acacggacga
     9061 tctgttcacc aagccagcga agaaacaaac agaagactac atcaccggtc gttacggttg
     9121 attcaggagt gcgttatgga cagtctcaat cttaataaac atatttccgg ccagttcaac
     9181 gccgaactgg aaagtatccg cacgcaggtg atgaccatgg gcggcatggt ggagcagcag
     9241 ctttctgatg caatcaccgc gatgcataac caggacagcg atctggcgaa gcgcgtgatc
     9301 gaaggcgaca agaacgtcaa catgatggaa gtggcgatcg atgaagcctg cgtgcgcatt
     9361 atcgccaaac gtcagccgac ggcgagcgac ctgcgactgg ttatggtgat cagtaaaacc
     9421 attgccgagc tggagcgtat tggcgacgtg gcggacaaaa tctgccgtac tgcgctggag
     9481 aaattctccc agcagcatca gccgttgctg gtaagtctgg agtcgctggg ccgtcatacc
     9541 atccagatgc tgcacgacgt gctggacgcg ttcgcgcgga tggacattga cgaagcggta
     9601 cgtatttatc gtgaagataa aaaagtcgat caggaatacg aaggtattgt tcgtcaactg
     9661 atgacctaca tgatggaaga ttcgcgtacc attccgagcg tacttactgc gctgttctgc
     9721 gcgcgttcta tcgaacgtat tggcgaccgc tgccagaata tttgtgagtt tatcttctac
     9781 tacgtgaagg ggcaggattt ccgtcacgtc ggtggcgatg agctggataa actgctggcg
     9841 gggaaagata gcgacaaata attcaccaga caaatcccaa taacttaatt attgggattt
     9901 gttatatata acttta
//
LOCUS       DAZKIV010000159         9815 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZKIV010000159 DAZKIV010000000
VERSION     DAZKIV010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.159"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1030..1560)
                     /gene="yodB"
                     /locus_tag="ACHOT6_003957"
     CDS             complement(1030..1560)
                     /gene="yodB"
                     /locus_tag="ACHOT6_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFP9949103.1"
                     /translation="MNRFTKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1903..2553)
                     /gene="zinT"
                     /locus_tag="ACHOT6_003958"
     CDS             complement(1903..2553)
                     /gene="zinT"
                     /locus_tag="ACHOT6_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFP9949104.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2810..3445)
                     /gene="msrQ"
                     /locus_tag="ACHOT6_003959"
     CDS             complement(2810..3445)
                     /gene="msrQ"
                     /locus_tag="ACHOT6_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFP9949105.1"
                     /translation="MRLTAKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3446..4450)
                     /gene="msrP"
                     /locus_tag="ACHOT6_003960"
     CDS             complement(3446..4450)
                     /gene="msrP"
                     /locus_tag="ACHOT6_003960"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFP9949106.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4559..4972)
                     /gene="hiuH"
                     /locus_tag="ACHOT6_003961"
     CDS             complement(4559..4972)
                     /gene="hiuH"
                     /locus_tag="ACHOT6_003961"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFP9949107.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            5105..5776
                     /gene="hprR"
                     /locus_tag="ACHOT6_003962"
     CDS             5105..5776
                     /gene="hprR"
                     /locus_tag="ACHOT6_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFP9949108.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5776..7134
                     /gene="hprS"
                     /locus_tag="ACHOT6_003963"
     CDS             5776..7134
                     /gene="hprS"
                     /locus_tag="ACHOT6_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFP9949109.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIQITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7242..8093)
                     /gene="hchA"
                     /locus_tag="ACHOT6_003964"
     CDS             complement(7242..8093)
                     /gene="hchA"
                     /locus_tag="ACHOT6_003964"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFP9949110.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(8685..9758)
                     /locus_tag="ACHOT6_003965"
     CDS             complement(8685..9758)
                     /locus_tag="ACHOT6_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9949111.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FIGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         2727 a         2009 c         2247 g         2832 t
ORIGIN      
        1 gagaaagact tggtttattg ttgattaatg gtatgacctc cgattttcat tgatagctta
       61 tgtgaaattc atattgtgct tgactttcga agtgattgag ctcagtgacg agatttattg
      121 gaattatagt taaacagaag agaggttaaa taccgtggag agctcgagtt atacaaggcg
      181 ttaacgtgag caataaatag gatgcgaagt aattttggtg gctcggaaaa ggcgttttct
      241 gtcgttatca attgttcggg tgcagtttta tttttaacct gtttgcttgg aatattctga
      301 ggttgtttga tgctaaccat ctgatttttc aatcctaaat atagaatatt tattccaaat
      361 aaaacacata atatattgat tttattaacc catagcatag gtctcgaaaa ccggagtagg
      421 ggcaactcta ccgggggttc aaatccccct ctctccgcca ctttatcaat gacttatctc
      481 ctgacttccc gccttgcttt tcctaaacag aacaatcgta gaatattctt gaagggttag
      541 atcgtcactg ttttctgttc gatactgtga cattcagcac ttgatccgct atggatctga
      601 caggaagctt tcgagcgaaa atgtgcagtt attcagtcgt tttcttatcg ctcaccatta
      661 ttcttttaga cattcatcct atgaaactgc cgcaaagttg gtggtgggaa ctgaagttgc
      721 gtagagaagg ggtcaataat tgagtcaaaa aaggaacgcg gccgtcagat taaagccgcg
      781 ggacaaagtc cataatttcc aagagttgtt gaatttccca gcttttttga aagcctttgt
      841 atcatactaa caagtaaaaa aggtttaaat accaaaatta acctgtattt ttgataagtc
      901 ttaccattgt acgatttaca cagtttctgc tcctccttgt tgatagtgga aagggtgcat
      961 tgtgcacttg cgtaaacatt tatgacgcgt tgatgtccat ggttcacatt tccttgtcag
     1021 gggatcgact tatttgcggc gaggcatcat tcgtaacaga gtattatctt tctgaatgta
     1081 gtgatgaaag agtgctgcgc cagcgtgagc tgcgattaaa aagtaaccta tatttgccca
     1141 ggtttcgtga atatttttta tcagtgcttt aatttcgctg tttggggtaa caaagggaga
     1201 cacattgaaa ccaaggaaac tccacgattt tccactgtaa gccatcaaag caatccccag
     1261 cagaggaagt gcaaggaagg ttatataaag catgatgtgc attagcgaag ccgctttcat
     1321 ttgccaggca ggtggcggtg gcacaataga aggatcacta taacggtgtt ttataatcag
     1381 gcgtgaaaac attaacaccc aaacgaatat acccgcattg taatgtgttt ctcgcatcag
     1441 cagataagta ctactacctt taggaaacca gccacggagt tccatcgcgg cataggttat
     1501 tgcaacgaaa agcagcgtta tccaatgtaa ataaatttga gtttttgtga atcggttcat
     1561 ttttgtagct cctgtaattt ttacagtcaa gctatcccac gaaccttaaa aattccttat
     1621 acagatgtgg ttttaagaat ttgttattta aaaaattgat agtgcacatt gtaaccagcc
     1681 aatataagca atggcgtgag cacaggtatc tttctctgtt ggccgtattg tgtatggaat
     1741 ccgttattgg ttaactcaat gcgggttagc ttattaatat acagacacgt catagtgatg
     1801 agcgtgtctg tagctagctt gcgttcagtg gcgtttgtgg atggaccagt ggcagttatg
     1861 atgacttact aaagcggcat cgaggcgtta tcatgagaat actcaatgag acatcatttc
     1921 ctcgaccact tcttcgctac tcaactgata tggataatac gttggccagt tttccatttc
     1981 atttaataaa gattgctgtg aatcattacc cataaaaatg tgaaaatgag atgattttcg
     2041 tggtgcaatt atatgatcgc taaattgtat atatttaggg gctttgcttt caggatcttt
     2101 acattcgaat aagtaacgga cgcctttctt gcctgattta taggtgagta ttttgtatcc
     2161 atcgtaatca tatttacagg atgttgtttc attatttcta tggaattcaa caatgccgtc
     2221 ctcaatgcca atcatctcga tatctgttgc ataacctttg tgataataat ctttaatttc
     2281 agcaaatgtt ttagttttat ctgcatccgc tttcttctga aagacggggt caagtttgcc
     2341 actttgcagt aaaggataaa cggattgcca gactccatcc cagtcactga gcgttcggtt
     2401 ttgtacattg gcatcatcaa aaacaccatt agcagctttt tgttcgacct ctgttaaggg
     2461 tttgccgtgt gaatgatgac catgcgaaaa ggcaggagcg ctaacaataa agacacctaa
     2521 agcaacagcc agtttgtgaa gacgaatcgc caaaacagtt tcctccagag tcattgtata
     2581 tgatgtgtaa tgttatattg taacatatga caaaatgcaa taactcttag gagatgagag
     2641 catagttttc tcaggtatac ttatctatct tattgaaaca aaatgaatta tgttgataat
     2701 ttgaggttga tgacaaacga aacataacct gatgtgatct gcgcaggccg gacagggcgc
     2761 tcgcgccaca tccggcaggg ataacgcgta ctttgtcggc aatatgaagt tatacagata
     2821 atttgttgtg aacctgcttg cgtaaccggt taaaaaggga acgcaacttc ttataccgta
     2881 aggctaaaag cagtacagcc agcccagcgt agatgagcgg ctgcggtgaa ataatcttca
     2941 ctgaccacag ataatgtatt ggggccagga tcgcgacaag atagacgaaa ttatgcaact
     3001 gttgccagtg cttgccgagt tttcgctgca tcgcctgggt tgaagtgaac gctaaagcaa
     3061 gcaaaattac ccagctgata atacctaacg ttaaataagg ccgggtgatt aactccttac
     3121 ctaataacgc cagattattc acgcccagct ccagcaatgc gtaactggtt aaatgcagtg
     3181 tcgcccaggc aaagcaccat aagcctaaca ggcggcgagt gcgtatcaat aacggctgtt
     3241 ttgcgtagcg tgccagaggt gtgattaaca aggtcgccag caaaaatttc agtgcagtgc
     3301 gaccggtaaa atgctgaata tctttcaccg gatcggcacc cagtccaccg tgattgatcg
     3361 cccagaccag ccagagaaac ggcaacaatc cggcaagatg caggcaaact ttcagccatg
     3421 tcacctgttt tgctgtcaga cgcattcaga aattctcccg caaatccagg ccacgataca
     3481 gcgatgccac ctggtcggcg taaccattaa acagtagcgt tggctggcgc tgtacatcga
     3541 ggatgccgcc tgaaccaata aatcgttcgg tagcctgtga ccagcgcggg tgatcaacat
     3601 gcggattaac gttggcgtaa aaaccgtatt cgtcaggcgc tgccagattc caggtggttg
     3661 gcggacgctc gcgggtcagc ttaatactga ctatcgattt aatcccttta aagccatatt
     3721 tccacggcac aatcagtcgc accggcgcgc cattttgtgg cggtaacgcc ttgccataaa
     3781 cacctacggt catcagtgtg agcggatgca ttgcttcgtc gagacgcaat ccttcgacat
     3841 aaggatattt cagcccgccg ccgataaagc ggtcctgctg gccaggcatc tgttccggtg
     3901 cataaattgt ttcgaaagcg acatacttcg cattgctggt gggttcggca agcgccagca
     3961 atttgtgcag cggaaaacca atccacggca ccaccatcga ccacgcttcc acgcagcgca
     4021 tacgataaat acgctcttcc agcgggaagc gacgggttaa atcatcgtga tcgagggtca
     4081 atggttttgc cacttcgccg ctgattttca gtgtccatgg atcggttttc aggctaccag
     4141 cattagcggc gggatcggct ttatccagcc cgaattcata gaagttgtta tagccggaaa
     4201 ctttatctgc tggcgtcagt ggcaggttat tttgccaggc ggcaggcttg ctgaactcca
     4261 gcggttttcc ggcgggggcg ggtgggcgat cgttcccttt aaaccagcta agcagatcgg
     4321 catgcgcagc gtgaggcaaa gaaagtgcag ctgcgctgat gcccagtgct tttaacacct
     4381 gccgacgctt cataaagaat accgactcgg ccgtaacatc tgattctttt aaaaattgat
     4441 tctttttcat aagccatcac acttttttgt cgttagccag caagagccgt ttactgttaa
     4501 tctgtcaggg agtgtcctcc ctaacagtag cagagatctg cttttacata tttatgcttt
     4561 aactgccacg ataggttgaa tacccatatt ggctaagtaa taaaggcaca tgataatgct
     4621 cgttcacttt attaatatga aattcaacgg ggatctcagg gaagaaactt tcaagatttt
     4681 gtttcttgaa atagtcccca gttttaaata cgacacggta atcgcccgta gttgcagttt
     4741 gctcgggcca cagtgcctta attcgtccat ccttatctgt tttggcggta ttaagttgta
     4801 accagccgtt gtccgctttc ttttcaagag tgactgtcac gtcggcagcg ggttttccgg
     4861 tttgctggtt caaaatgtgc acgctaagaa tgttttgttg tgcggcataa accaaagaag
     4921 gtaatgaaaa tgctgccgtt actacggaga gtactaaata acgctttaac atgtttatat
     4981 ccttgtcatg tgaatgagtt ttgaggatag cgaaaaagcg gccagggtaa ccttacagca
     5041 gcattacaat tttgtaatga accaggctgt ttctataaca tatgatttat ggcatattat
     5101 tttcatgaag attttactta ttgaagataa tcaaaggacc caggagtggg taacgcaggg
     5161 gctttccgaa gcgggttatg tcattgatgc cgtttctgat ggcagagatg ggctttatct
     5221 tgcgctgaag gatgattatg cattgataat tctggatatt atgcttccgg gtatggatgg
     5281 ctggcagatc ttacaaacgt taagaacagc aaagcaaacc cctgttattt gccttactgc
     5341 aagggattct gtcgatgaca gagtcagagg gctggacagt ggagcaaatg attatctggt
     5401 aaaacctttt tcattttctg agttgctggc aagggttcgg gcacaattaa ggcaacatca
     5461 cgctttgaat tcaacattag aaatcagtgg cttaagaatg gactctgtta gtcaaagtgt
     5521 gagcagggac aatatcagta ttacactgac gcgcaaggag tttcagttac tttggctact
     5581 ggcctccaga gctggcgaaa ttatacccag aacggttatt gcgagtgaaa tttggggaat
     5641 caactttgat agtgatacca atacggtgga tgtcgccatt cgcaggctcc gcgcaaaagt
     5701 tgatgatcct tttcctgaaa agctaatcgc cacaatccgg gggatgggct attcattcgt
     5761 agcggtaaaa aaataatgaa aagattatct ataaccgtcc gtttaacctt gctttttata
     5821 ttgctgctgt ctgttgctgg cgctggaatt gtatggactc tctataatgg cctggcaagt
     5881 gagttgaaat ggcgcgatga tacaacactc attaaccgga cagcgcagat caagcagttg
     5941 ttaattgatg gggtaaatcc agatacgtta cctgtgtact ttaaccggat gatggatgtt
     6001 agtcaggata tcttgatcat tcatggtgat ggcatcaata aaattgttaa ccggacgaat
     6061 gtcagtgatg acatgttaaa taacatacct gctagtgaga caatcagcgc agctggcatt
     6121 tacagaagca ttattaatga tacagagata gatgctttac gaattaatat tgatgaagtt
     6181 tcgccatcat taacggttac tgtggctaaa ttggcttcag ccagacataa catgcttgaa
     6241 cagtataaaa ttaatagcat tataatttgc attgtcgcca ttgtactttg ctcagtatta
     6301 agtccgctgt taatcagaac gggattacga gagatcaaaa agttgagtgg tgtaacggaa
     6361 gcgctgaatt ataacgatag ccgagagcct gttgaggtta gcgcattacc gagagaatta
     6421 aaacctcttg ggcaggcgtt gaataaaatg catcaagcct tagtcaaaga ttttgaacgc
     6481 ctaagtcaat ttgctgacga tctcgctcat gaacttagaa cgcccattaa tgcattactg
     6541 ggtcagaatc aggttacgct cagtcaaacc agaagtatcg ctgaatatca aaaaacaatt
     6601 gccggtaaca ttgaagagct ggaaaatatt tcgcggttaa cagagaacat actgtttctt
     6661 gcccgggcag ataaaaacaa tgttttggtg aaactggact cgctttctct caataaggaa
     6721 gtcgaaaatt tgttggatta tcttgaatac ctttcagacg agaaagagat ttgctttaag
     6781 gtcgagtgca atcagcaaat ctttgcggat aaaattttac tgcaacgaat gttatcgaat
     6841 cttattgtta atgccattcg atattcacca gaaaaatcgc gtattcagat aaccagtttt
     6901 cttgatacta acggctatct taatattgat gtcgccagtc ctggaacgaa aattcatgag
     6961 cctgaaaaac tcttccgtag attttggagg ggagataatt cgcgtcattc cgtaggtcag
     7021 ggacttggtc tttctttagt caaagcgatt gccgaattac atgggggaag tgctacgtat
     7081 cactatctca ataagcataa tgtgttccgg attacgttac cgcaaagaaa ttaacatagg
     7141 gcttcagtac gccataaatt aaccgtgaaa aaacgcagcc acgtcgtgag tactaacgcg
     7201 gctgcgatta attatgcgct tacattcaaa cgtaacaggg attaacccgc gtaagctgcc
     7261 agcatttcct gcgccgctag tttacccaac gcattcgctg cgaaaggact gtcgccggtg
     7321 agaagtttac ggtccttatg tactcgcccg gtgatgtcgt cattaatgat gttcatgccc
     7381 attttcttca gctcttcgcc gaagtaccag gtgagatgac ctggcatata gccaatatct
     7441 ggcgtttgtt tgtctgcggc gtctgggaat gcgcaaatgg aataaccatt cagtgggtta
     7501 tcgccgtggc gaagcgccag aaaagccgcc gggccgtggc aaagggagat aacaaaacgg
     7561 tcatttttga ttgcccactg taaagcggca gctacgtcct ggctttcagg taaaccaata
     7621 agtgcgccat gaccaccagg aacaaagatt gctgcatatt cgctatcagc gttgaggctg
     7681 gcgacaacat ccgcgagttt cttcggattg cggaacaacg atttatgctg ctcaaagaat
     7741 ggcattactt tttcatcttt gtgcggcata gcccagtatt caaacttggt catcagaccg
     7801 gaaatggtcg ccacttcgaa ttcgaaacct gcagcatgga gatgatacaa cggcagcaac
     7861 gtttcaatcg gatggttacc ggtcgagaac agttttccgt tatcggttgg caaataacgt
     7921 tcgtccgcgg cgatcaccag aattttatgc ttaccgcgat acggttttgg atagtccacg
     7981 ccatcaagat cagagacggg actggtatat tggctaagcg aatattctga agggaagaac
     8041 gcattatctt cagcaatatc gacctgcgga tttttacttg tttgaacagt catagtgtat
     8101 tccttattgt tgcttagtta gggtagtcac tatatttgcg actgagctgg tcgctatttt
     8161 taaccaggct ggaaaattga gtcaatatat cgaaagcggt agtcggcggg tgtaagtatg
     8221 taatgatgat gctgcactgg tgttgaatat tattcacgcc aaattttcct tattgagcaa
     8281 cgtcgtcgca agtggcctca atgattttag accattatca aaggtacggg gcggcgggga
     8341 gagatttagt gctgtgaaat attgtggtac tgatgaatat ttatatcgcg gattggtaga
     8401 gaaaagatta tttcatgcac tatttttgca aatatttcta tatgggaagc gtgtttgtta
     8461 cggggtaatt catcagaatg tgcatttggg gtgttggctg gaagagtcgc tggtagatgt
     8521 tattgtggca ggaggggcag ggtaaggtgc tgtatcgata tgctttggca attgcgtgtg
     8581 ccgcagatgg agatgaaatg ccaataaaaa agcctgagtt cgatgaggga cgaactcagg
     8641 ctagtgttat gttttacagt aacacgccgc ggcgcgttgg ctgattagaa ctggtagacg
     8701 ataccaactg caaactggtc atcagttgcg gcatcggtaa tcgctgcatc gtctttatcc
     8761 agcaggttaa atttgtatgc tgtgtagacg ttcatgttct tattgaagta gtaccaggta
     8821 ccaacttcga tataatttac gcggtcagca tcaacacctt tatgtccatc acgagcataa
     8881 agatctttgc ctttggaata tacgtagcct aaagacggac gtaaaccgaa gtcgaactga
     8941 tactgtacaa cagcttcaaa gttttgggtt ttatttgcaa aatgatcatt agccaaaacg
     9001 gtcatgttgc gggtttcacc gtacatcatt gctgcataga tattatttgc atcatacttg
     9061 gctgctaatg accacacttc agcgttatca ccgtagccgt caccagcttg ttcattagta
     9121 cgatcagatt tgctatatgc acctacaaaa ccaaaacctt cgatgttgta gtctacagac
     9181 aacgcatagc catcaccgtt ttgtttagta acgccacgat tgctgttctt accttgatat
     9241 tgcacagcaa aattcagacc atcaaccaga ccaatgaaat cagagttacg gtaggttgca
     9301 acaccgttgg tacgaccgtt catgaagttg tcagcggaag cccaggaatc accaccccat
     9361 tcaactaaca tatcggtata agctgcggca tcataagcaa caccgtagtt acgaccatag
     9421 tcgaaagatc ctaactcgtt atatttcaaa cctgcgaagg ttaaacgggt tttttcctga
     9481 ttagagcctt cgggcttgct ggcgtcgagg ttgtattcaa actgaccaaa accagtcagt
     9541 tcgctggtga tttgggtttc acctttaaca ccgaaacgag cataggaggt atcttcgttt
     9601 tcgctgttat tatcatcagt atttgaccag atgcgttcgc caaccatctt gccgtagaaa
     9661 tcaactttat tgccatcttt attgtaaatt tcagccgcat ttgctgcgcc agcaactaat
     9721 aacgccggga ccagcattgc cagaactttt cttttcatta tgtattccct tgtgattata
     9781 atcttcatga atatatcaat aagtgccgtt atcca
//
LOCUS       DAZKIV010000160         9697 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZKIV010000160 DAZKIV010000000
VERSION     DAZKIV010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9697
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.160"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..1606)
                     /gene="yeeO"
                     /locus_tag="ACHOT6_003966"
     CDS             complement(119..1606)
                     /gene="yeeO"
                     /locus_tag="ACHOT6_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFP9949112.1"
                     /translation="MWFHFLQFRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFNPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1767..1842
                     /locus_tag="ACHOT6_003967"
     tRNA            1767..1842
                     /locus_tag="ACHOT6_003967"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Asn,seq:gtt)
     gene            complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACHOT6_003968"
     CDS             complement(1880..2830)
                     /gene="cbl"
                     /locus_tag="ACHOT6_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFP9949113.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSSEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACHOT6_003969"
     CDS             complement(2932..3849)
                     /gene="nac"
                     /locus_tag="ACHOT6_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFP9949114.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4175..4250
                     /locus_tag="ACHOT6_003970"
     tRNA            4175..4250
                     /locus_tag="ACHOT6_003970"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4208..4210,aa:Asn,seq:gtt)
     gene            complement(4309..4869)
                     /gene="hxlB"
                     /locus_tag="ACHOT6_003971"
     CDS             complement(4309..4869)
                     /gene="hxlB"
                     /locus_tag="ACHOT6_003971"
                     /EC_number="5.3.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0043800 - hexulose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HFP9949115.1"
                     /translation="MSVQENTINILHELESNALKIDDAQAAQFISQIRNARHIFLQGA
                     GRSGIAIRAFANRLLHLGFSVSVVGEISSPHTQPGDLLIIGSGSGETTSLKSLAQKAV
                     DSGVNVALVTMKADSTIGKLAQSVLVLPGSVKDDNSRVAGAFAQPMGSAFEQLCFITY
                     DAIVLELMSELGESSATMFTRHADLE"
     gene            complement(4885..5637)
                     /locus_tag="ACHOT6_003972"
     CDS             complement(4885..5637)
                     /locus_tag="ACHOT6_003972"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HFP9949116.1"
                     /translation="MSNFAGKVVLVTGGAHGIGKGIAKQFADRGAHTVIVDYNEENGK
                     ATAAELTFGEVKSLFIKTDVSDPQALVDVREKVFATFGRIDVLILNAGVAFANKVNNI
                     SFEEWNKTIAINLSGLFNTVKAFYDDFLTNKGSIVYISSGSALSGTGGGVSYPASKAG
                     GEGLIRGLAKELGPKGVNVNAIAPRLIDTGEMMRVNYPTQADLDAVLEKIPVRRLGTI
                     DDVANLAIFLADKDNSYIQGQTILMDGGRTIA"
     gene            complement(5735..6379)
                     /gene="rpe"
                     /locus_tag="ACHOT6_003973"
     CDS             complement(5735..6379)
                     /gene="rpe"
                     /locus_tag="ACHOT6_003973"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001062775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFP9949117.1"
                     /translation="MAKILPSIFGADILRLKDEIHFLEEEQTEILHVDLMDGTYVSNI
                     AFGPNQIAAMKKASSMIFDVHMMLAKPERHIDDVINTGAEMISVHYESTPHVHYIIQK
                     IKKAGRKAGVVLNPGTPESVIEYLLDDIDYILLMTINPGQPGQTFIHKSLEKIRNAKK
                     MISGRDIQIEVDGGVNLDNVKQVKEAGADLIVVGGALFNKQPRESYQALKIAVQ"
     gene            complement(6402..7655)
                     /locus_tag="ACHOT6_003974"
     CDS             complement(6402..7655)
                     /locus_tag="ACHOT6_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HFP9949118.1"
                     /translation="MEMLKNLMETITGMGATAILPLVIFILGLVFRMKPGAAIKSGIT
                     VGIGFIGLGLVVGLLNSSLQPAIEYYSKVGSGFTVADIGWPAVGAAAWVAPFAALVIP
                     VGIVLNLILVRLKLTKTLNVDIWNYMHFLVPGALAYFVFDSFIIGFSVAVALSIAALF
                     IGDLIAPRWQKYYGLEGTTCTTMIHIGWTLPFAWVVNKIIDYIPGLNKLDVDLNSVQK
                     RLGVFGEPAIIGVIVGALLGVLTKQAITTIVPMAMGVAGVMVLLPKVVGVLMEGLNPI
                     GKSAKEIMQKQMGKDAELNIGMDCALALGDPATVTVTVITIPLTMLCALVLPDIKIFP
                     IGVLMSIIYMTTMTVMASNGNVIRSITSTLLFCVVVMYLGGYVAPGATQFLAGAGVGL
                     QGQGTDFVLTGPWEILTYWLSTVLH"
     gene            complement(7676..7969)
                     /locus_tag="ACHOT6_003975"
     CDS             complement(7676..7969)
                     /locus_tag="ACHOT6_003975"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9949119.1"
                     /translation="MINILVACGSGVATSTLAADEVKSVCAEYGITAFKINKCSMAEL
                     SSEMAHADIVLTTNNYKGDIGKPHMSVAGFVTGINEGALRKKLGELLTELANS"
     gene            complement(7988..8431)
                     /locus_tag="ACHOT6_003976"
     CDS             complement(7988..8431)
                     /locus_tag="ACHOT6_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFP9949120.1"
                     /translation="MISISVADGAGLQNYDAVLEHIFNTHRTTNTVKESWLNAMRKRE
                     AEYPTGIELEGYSIAIPHCDSEHANQPAIYIIRLPDAIPVNRADEDGILWVRLVVNLI
                     VTDPADQLRLLKSLFNHLQNEEFYQNLLTLPVDDAKALFVSKIIQ"
     gene            complement(8759..9514)
                     /locus_tag="ACHOT6_003977"
     CDS             complement(8759..9514)
                     /locus_tag="ACHOT6_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFP9949121.1"
                     /translation="MYLVDERRKLILEDLMSAGTVYVSDLARKYEVTYETIRKDLTHL
                     EQKGLLIKSHGGATLKQRAIEHPFQVREKENNSYKKAIARKALDFIPPNSSMIIGTGS
                     TTFELANLLCMKSGFKIFTDSLPVASVLIESDNQVFLFGGELRRKSSSVFGGWAVSMI
                     NDIHVDLCFIGTDGFSNMTGPSTPSSSDAFLDRTIISHSDKKYILGDYTKFERSSLFK
                     ICDWSEITALITNDHADKEGIAEVAKYTSVITC"
BASE COUNT         2853 a         2399 c         1886 g         2559 t
ORIGIN      
        1 cgactgaact acagaggaat cgtgagaacg aggcgaatat tagcgatgcc cacccacaat
       61 gtcaaagcct gttttttaaa tttgaaatcg tttgctgaaa taatctgcat tttgtcgttt
      121 attccgacac aactggcttt ttctcacact tttgcggctc ggatcgaggg tatttccata
      181 gccaacgtcc agtaaccatt cgccagtaaa acagcacggc ccgcacagcc cagtcggcaa
      241 acattcccat ccagacacca accacacccc agccaagcat gatgcccagc acataaccga
      301 cgacaacccg gcaaccccac atgctcaaca tagaaaccca catagcgtaa cgggcgtcac
      361 gagccccctt aaatccagcg ggtagccccc atgaggcgga ccaaataggc ataaataaag
      421 catttagcca aatcagaatc acaacgacat gtttaacctg tggatcctgg gtgtaaaacg
      481 atgccataac cccggcaaag ggagccgtta gccaggcgat ggccgttaat ccaagagtgg
      541 aaagccagaa cacatgccgc aactgaatct ctgcttgcgc tatctgccct acccccaacc
      601 ttcggcctgt aatgatcgta gaagcagagc cgagcgcact tccgggtaag ttgataagag
      661 ccgcaattga aaacgcgata aaatttccgg caataacact ggtccccatc ccggcaacga
      721 acatttgggt taataaccga ccactggtaa ataacactga ttcgacactc gcgggaatac
      781 caatccccat gacttcccag ataatgctaa aattcagtgg gttaaaatag ctctttaacg
      841 aaatccttag cgcaggatta aaaccaatcg ccaacaccca caaaattgca actgcaccaa
      901 tataacgaga aatggttaaa cccaacccag ccccgacaaa tcccagtccc ggccaggaga
      961 aaaggccgta aatcaatatg ccgctaataa taatattaag aatattcagg ctaccgttaa
     1021 tcagtagcgg tattttcgta ttccctgcac cacgaagtgc cccgctacca attagagtga
     1081 tggcagctgc tggataactg agtaccgtca gctccagata agtcaacgcc agtgctttaa
     1141 cttctgtcgt ggcatcaccc gcgacgaaat caataatttg ttcgccaaaa tgatgaataa
     1201 gcgttgccaa cagtacggca aacaacgtca tgatcaccaa tgactgccgc gtcgccaccc
     1261 tcgctcgtcg tcgatcccgc ttaccgagac taaatgccac aacgacagta gtaccaagat
     1321 cgatagctgc aaaaaaagcc ataatgacca tattgaagct gtccgccaat cccacgccgg
     1381 ccatcgcatc ttttcccagc cagctgacca gaaaagtgct caggacaccc atcagcagga
     1441 cacaggcatt ctccatgaag ataggaacag caagcggggt tatctcgcgc cagaacaaca
     1501 ctttgtagct cttgcgttta gcgtgccagc gagtgccgtg aacaacctgg cgtaaagcag
     1561 aggagatatt caaagccgac ctaaattgca gaaagtgaaa ccacatttca aataatgagg
     1621 gagaatcagc aaagctgcaa agattttcgc caacaaattg tctgcaaatg caacaaactg
     1681 ttgatagaaa cggcaaacag ttggggaatt taaaaatcgg gtttgacaaa agatttttcg
     1741 ccgttaagat gtgcctcaac agcgattcct ctgtagttca gtcggtagaa cggcggactg
     1801 ttaatccgta tgtcactggt tcgagtccag tcagaggagc caaatttctg aaaagcccgc
     1861 ttttatagcg ggctttttgc tatatctgat aatcaatttc ctcttcactg ttttccatca
     1921 cctgccgctt gatatcctca acggacagtc ctgcattaca aagttccaga aagcgccaga
     1981 catagttacg ctgaagttgt cctcgcttga gtcccaacca gacagtatta gcatcaaaaa
     2041 gatgccgcgt atccaggcgg attaaattct cttcctcttg ttcgctgctg gattgttcgg
     2101 caactaatcc gatcccaagc ccaagagcaa cataggtttt aatgacatca gaatcctgcg
     2161 cacttaatac aatatctgcc agcaaacctt tgcgggcaaa tgcgtcatca atacgtgagc
     2221 gccccgtaat cccctgtcgg taagtgatta acggccactt cgctattgat tccagcgtca
     2281 atggtgaaat ttgcgtcaag ggatgatcgt gtggaacaag caaactatgg tgccaacgaa
     2341 accacgggaa ggcgacgagc tgcgggtcat tactcaaacg ctcgctggcg ataccaatat
     2401 cagcttcgcc attttgcaac aacgtcgcaa tttcctgtgg cgtcccctgg attagctcga
     2461 gccgtacctc cgggaaaagt tcgcgaaaag ctttaatgac ctctggcaag ctataacgcg
     2521 cctgagtatg cgtcgttgca atagtgagaa cgccagacgt atcgttggta aacaggtctg
     2581 caagccgacg aacattactg gcttcattca gaatacgttc tgcaatgacc agtaatgctt
     2641 tgcccggttc agtcatgccc agcagtcgct tacctcgtcg aacaaatatt tcgataccaa
     2701 gttcatcctc cagttcccga atatgacggc tgacgccgga ctgtgaggta aaaagcatat
     2761 tcgcaacctc tgtcaggttg taatcctgac gtgcagcctc gcggattatc tttagttgtt
     2821 ggaaattcac ggtaaactcc gggcagttca gatttcccgt tattgttaaa gtctaatgcc
     2881 cggcataaca aataataaaa acccgcatct tattccatcc cgatataacg cttagctcac
     2941 caattgccac tgcctttttt ccatcactgg agaactaatc actgacatta acaactcttt
     3001 cactgcctgt gcctgtggtg ataagttcgc tctggcgggt aaatttaatg acaaagagag
     3061 actcatggaa ggagtggtaa tgcgtgacat ccaaccattt actgcgccac ataacgaacg
     3121 cgcggccgat tcgggtaata ctgcaacgcc catgccgctg gcaatcgctg cggtaagcgt
     3181 ggcaatagac tcaatttcac caatgacttt tgccgtgagc cgccgtaggg aaaaagcctc
     3241 atcaacacga agcctaacag cactgtaatc actggggaga aagaggttca tttgcgcaat
     3301 agcattaaca tcaacgcttt gccccggaca atcttgagtt cctaccagga aaagatcttc
     3361 tttcagcaaa gcctgactgg atacaccagc cacaggggaa tgctcataaa tcaccgccat
     3421 atcgagttgg tgatttatca gtttttcgtt aagcactgca ccgctatttt catgaagata
     3481 gataacgatc tccgggaatt cagctcgaac cgcctgtaat aagggcatgg tgatggatga
     3541 cgcagcggtt cctggcgcaa agccaatcga gacttgcccc gataatgcct gaccaacgtt
     3601 atgcaccgcc agttgggcct gctcacactg acgtaaaatg gcccgcgcat gggtatagag
     3661 aatttttccg gcgtctgttg gcgtaacgcc ccgttttgtg cgaatcaaaa gctgttgatt
     3721 taactcacct tccagtgtgg caacctgctg gctgagcgct ggttgtgcga tatgcaatac
     3781 ttcagcagcc tgcgtcaggc taccaatatc tacaattttt acgaagtatt tcaggcgtct
     3841 gaagttcatg ttgcctccgg tttttaagaa tcggcccaag tgccgccatt acttacaacc
     3901 agactgcaag atgcttgcca gttttgcttt ggcgttgatg tacaagctaa ccaactgtca
     3961 aataagagat tatgatagat tcgtcatagg ttctttttat cagaagtcgc gttcccctgc
     4021 cccataaaag gagggtatgc accacgatgg ttcattacca ataagattga aatctcaccg
     4081 ctttgttgaa atagccagca aacaaacaaa aaaaagcatt tcaccctttg acatcaccat
     4141 gcactgccat taatatgcgc cccgttcaca cgattcctct gtagttcagt cggtagaacg
     4201 gcggactgtt aatccgtatg tcactggttc gagtccagtc agaggagcca aattcaaaaa
     4261 agcctgcttt ctagcaggct ttttgctttc taattaccaa ctctttggtc attccagatc
     4321 agcgtgtcgg gtaaacattg tggcactgct ttcacccagt tcagacatca actccagaac
     4381 aattgcatca taagtaataa agcagagttg ctcaaaagct gagcccattg gttgtgcgaa
     4441 agcgcctgca acacgactgt tatcgtcttt cacacttcct ggtagtacca acacagattg
     4501 agctaattta ccgatcgtcg aatctgcttt catagttacc agagctacgt ttactccgct
     4561 atcaactgct ttttgtgcca gacttttcaa actggtagtt tcaccactgc ctgacccaat
     4621 aattaataga tctccaggtt gagtatgcgg tgatgatatt tctcccacaa cgcttactga
     4681 aaaacctaaa tgcagcaagc gattagcaaa agcgcgaata gcaatgccac tgcgaccagc
     4741 accctgcaaa aaaatatgcc gggcatttct gatttgcgaa ataaactgtg cagcctgagc
     4801 atcgtcaatc ttcagcgcat tactctctaa ttcatgcaga atattaatag tattttcctg
     4861 aacactcatt ttttctctcc ctttttaagc aatagtccgg ccaccatcca ttaaaatggt
     4921 ttgtccctga atataagagt tatctttatc tgccaaaaat attgccaggt tagccacatc
     4981 atcgatagtc cccagacgac ggacgggtat tttttccagg acagcgtcta aatcggcttg
     5041 tgttggatag ttgacccgca tcatctctcc ggtgtctatt aagcggggag caatcgcatt
     5101 aacattgacg ccttttggtc ctaactcttt tgccagacca cgaatcaatc cttcgcctcc
     5161 cgctttactg gcaggataac tcacaccgcc cccagtcccc gaaagtgctg atcccgatga
     5221 gatataaaca atagatccct tatttgtcag gaaatcatca taaaacgcct taacggtatt
     5281 aaataatccc gataagttaa ttgcgatagt cttattccat tcctcaaaag aaatattgtt
     5341 aactttattg gcaaatgcca caccggcatt taatatcaac acatcaatgc gaccaaaggt
     5401 agcaaatacc ttttcccgta catccactaa tgcctgtgga tcggagacat ctgttttaat
     5461 aaacaacgat ttaacttcac caaaagttaa ctctgctgca gtggccttgc cgttctcttc
     5521 gttatagtca acaataacag tatgtgcgcc acgatcagca aattgtttag ctatcccttt
     5581 gccaataccg tgagcacctc cggtaactaa aaccacttta cctgcaaaat tactcattac
     5641 ttttctccat taaagacatt ttttagctga ggttttcctc ccgatatgaa atcgaaaacc
     5701 tcacttaact ggatgtgaaa tttttagatt gtgatcactg aacagcgatt tttaatgcct
     5761 gataactttc acgaggctgt ttgttaaata aggcaccacc aacaacaata aggtcagccc
     5821 ctgcttcttt aacttgtttt acattatcaa gattaacgcc accatccact tcaatttgaa
     5881 tatctctacc agatatcatt tttttggcat tacggatttt ctccagactt ttgtgaataa
     5941 atgtctggcc tggttgcccg gggttaattg tcattaacaa tatataatca atatcatcta
     6001 ataaatactc gatgacagat tcaggtgtcc caggattaag cactacacct gctttgcgac
     6061 ccgctttttt gattttttga ataatataat gtacatgtgg cgtcgattca taatgtacag
     6121 aaatcatctc cgccccggta ttgattacat catcaatatg tcgctcaggt tttgccagca
     6181 tcatgtgcac gtcaaagatc atcgaactgg cttttttcat tgctgcaatt tgatttgggc
     6241 caaaggctat attactgacg tatgtgccat ccattaaatc aacgtgcaat atttcggttt
     6301 gttcctcttc cagaaaatga atttcatctt tcaggcgtaa aatatccgcg ccaaatatag
     6361 aaggtaatat ttttgccatg ttaaaactcc agattattaa atcaatgtaa tacggtactc
     6421 aaccaatagg ttaaaatttc ccacgggccg gttaatacaa aatcagtacc ttgtccttgc
     6481 aagcctacac cggctccagc taaaaattgc gttgcccctg gtgcgacata accgcctaaa
     6541 tacatcacta caacgcagaa taacaaggtc gaggtaatcg aacgaatcac gttaccgttg
     6601 ctcgccatta cggtcatggt ggtcatataa ataattgaca tcaatacgcc aattgggaag
     6661 atcttaatat caggcaatac cagagcacat agcatggtta aaggaatggt aattactgtc
     6721 acggtgaccg tcgccggatc ccccaacgct agagcacaat ccataccgat gtttaattca
     6781 gcatctttac ccatctgttt ttgcatgatt tctttggcac ttttccctat tgggttaaga
     6841 ccttccatca gcacaccaac aacttttggt aacaacacca tgacgccagc aacccccatc
     6901 gccatgggaa caattgtggt aattgcctgt ttcgttaaaa ctccgagtaa tgcgccaacg
     6961 ataacaccga taattgcagg ttcaccaaag acacccaggc gtttttgcac actgtttaag
     7021 tcaacatcta acttatttaa ccctgggatg taatcaatga ttttatttac tacccaagcg
     7081 aacggtagag tccagccgat atgaatcatc gtggtacagg ttgttccctc gagtccataa
     7141 tatttttgcc atctgggagc aatcaaatcg ccaataaaca gagccgcaat actcaaagcc
     7201 accgcgacag agaagccgat gataaaacta tcgaacacaa aatatgccag agcgcccgga
     7261 actaaaaaat gcatatagtt ccagatatca acatttaacg ttttggttaa tttcagacgc
     7321 accagaatca ggttaagtac gatgccaact ggtatcacta atgcggcaaa aggtgccacc
     7381 catgcagcag caccgactgc tggccaacca atatctgcaa ccgtaaaacc actacctact
     7441 tttgagtaat actcaatagc tggctgtaat gaactattta ataaaccaac aaccagacct
     7501 aaaccaataa aaccaatacc gactgtgatg cccgacttga ttgcggcacc tggtttcatt
     7561 ctgaacacca gacctaaaat aaatatcacc agcggcagta ttgcagttgc ccccattccg
     7621 gtgattgttt ccattaaatt tttcaacatt tccattttta ttattcctgt caggtttagc
     7681 tatttgctaa ttcagttaat aactctccta atttcttgcg taatgctcct tcattaatgc
     7741 ccgttacaaa acctgcaacg ctcatatgtg gtttcccaat atcaccttta tagttattag
     7801 ttgtgagtac aatatctgca tgagccatct cagaagaaag ttcagccata ctgcatttgt
     7861 tgattttaaa tgccgtaata ccatattcag cacatactga cttcacttca tcagcagcaa
     7921 gcgtagaggt agcgacaccg ctaccacaag caacaagaat attaatcata ataaactcca
     7981 gtttttacta ttggattatt ttggatacaa aaagtgcttt tgcatcatcg acgggaagtg
     8041 tcagtaagtt ttggtaaaat tcttcatttt ggagatggtt aaataatgac tttaacaatc
     8101 gaagctggtc agcaggatcc gtaacaataa gattaacgac aagtctgacc cacaatattc
     8161 catcttcatc agccctgttc acaggtattg cgtctggcag acggatgata taaatagcag
     8221 gttgattcgc atgttcacta tcacagtgtg gtatagcgat agaataacct tccagttcta
     8281 ttcctgtcgg atattccgct tcgcgtttac gcatagcatt gagccagctc tctttcaccg
     8341 tattcgttgt acggtgcgtg ttaaaaatat gctcaagcac ggcatcgtaa ttctgcagtc
     8401 ccgcaccatc ggcaacactg atcgatatca tatcctttcc ccctcaccca aaaatactca
     8461 catgagcatt gaacaatatt catatgagca gttatagcac aacgatcctg tggataaata
     8521 acgagatctg agaaattaaa tccagatcac aaaaaagcac ataatggcgt cagtatttat
     8581 gctcatatga gcaaatcgtg atttcttcgc aaaaaagtaa ctgaaactgg caataggcag
     8641 aggatgtatt caatagaggg cgctaagcaa gcatcgccat ggatgctcac ttgataattg
     8701 ggaaatgatt acatttggca aagctgattc gaagggagaa ggcgtgtcgt agtgagttct
     8761 aacaggttat tactgacgtg tattttgcga cttcggcaat cccctctttg tcggcatgat
     8821 cgttggttat aagggcggtg atctccgacc agtcgcagat cttaaataaa ctgctgcgct
     8881 caaacttggt gtaatcaccg aggatatatt ttttgtctga gtgcgaaatt atcgtacgat
     8941 ccaaaaacgc atcagaagaa gaaggcgtcg atggaccagt catgttacta aagccgtcgg
     9001 ttccaataaa acagagatca acatgtatat cgttgatcat agataccgcc caccccccaa
     9061 acactgagga actctttcta cgcagttcgc cgccaaagag aaaaacctgg ttgtcagatt
     9121 caatcaacac actggcaacg gggagcgaat ctgtgaagat tttaaaccca gacttcatac
     9181 ataacaggtt agccagctca aaagtggtac tgcccgtacc gataatcatc gaactattag
     9241 gcggaatgaa atcaagcgct ttacgagcta tcgccttctt ataagagtta ttttcttttt
     9301 ccctgacctg aaagggatgc tctatagccc tttgctttaa agtcgcccca ccatggcttt
     9361 tgatcagcag ccctttttgt tcaagatggg tcaaatcttt cctgatggtc tcatacgtta
     9421 cttcatactt tcgtgcaaga tcactgacat agaccgtacc tgccgacatt aagtcttcaa
     9481 gtattaactt tcgcctttca tcaaccagat acatccttcc tcctgaaatc tattacccat
     9541 cttagaaaac gccaagcata gcacagatga tgcaaacaaa aacaaacact aacagtggaa
     9601 agtacaaata aacccaataa agacaacagt tcaaaaacca tatatccgat tgataataaa
     9661 tgagttttta ttattcatta caacgccact gtacaat
//
LOCUS       DAZKIV010000161         9549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZKIV010000161 DAZKIV010000000
VERSION     DAZKIV010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.161"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..301
                     /gene="rcsA"
                     /locus_tag="ACHOT6_003978"
     CDS             <1..301
                     /gene="rcsA"
                     /locus_tag="ACHOT6_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFP9949122.1"
                     /translation="KPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAG
                     QGTIQISDQMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(345..533)
                     /gene="dsrB"
                     /locus_tag="ACHOT6_003979"
     CDS             complement(345..533)
                     /gene="dsrB"
                     /locus_tag="ACHOT6_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFP9949123.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            696..923
                     /gene="yodD"
                     /locus_tag="ACHOT6_003980"
     CDS             696..923
                     /gene="yodD"
                     /locus_tag="ACHOT6_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFP9949124.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            1221..2036
                     /gene="yedP"
                     /locus_tag="ACHOT6_003981"
     CDS             1221..2036
                     /gene="yedP"
                     /locus_tag="ACHOT6_003981"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFP9949125.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPAHVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(2033..3727)
                     /gene="dgcQ"
                     /locus_tag="ACHOT6_003982"
     CDS             complement(2033..3727)
                     /gene="dgcQ"
                     /locus_tag="ACHOT6_003982"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFP9949126.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(3898..4080)
                     /gene="yodC"
                     /locus_tag="ACHOT6_003983"
     CDS             complement(3898..4080)
                     /gene="yodC"
                     /locus_tag="ACHOT6_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFP9949127.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(4159..5076)
                     /gene="yedI"
                     /locus_tag="ACHOT6_003984"
     CDS             complement(4159..5076)
                     /gene="yedI"
                     /locus_tag="ACHOT6_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFP9949128.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5249..6169
                     /gene="yedA"
                     /locus_tag="ACHOT6_003985"
     CDS             5249..6169
                     /gene="yedA"
                     /locus_tag="ACHOT6_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFP9949129.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(6158..6628)
                     /gene="vsr"
                     /locus_tag="ACHOT6_003986"
     CDS             complement(6158..6628)
                     /gene="vsr"
                     /locus_tag="ACHOT6_003986"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFP9949130.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(6609..8027)
                     /gene="dcm"
                     /locus_tag="ACHOT6_003987"
     CDS             complement(6609..8027)
                     /gene="dcm"
                     /locus_tag="ACHOT6_003987"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFP9949131.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(8094..8789)
                     /gene="yedJ"
                     /locus_tag="ACHOT6_003988"
     CDS             complement(8094..8789)
                     /gene="yedJ"
                     /locus_tag="ACHOT6_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFP9949132.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFAQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPLAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTVRGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSSAG"
     gene            complement(8829..9110)
                     /gene="drpB"
                     /locus_tag="ACHOT6_003989"
     CDS             complement(8829..9110)
                     /gene="drpB"
                     /locus_tag="ACHOT6_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFP9949133.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         2326 a         2559 c         2294 g         2370 t
ORIGIN      
        1 taaaccggaa tctctcgacg atatccttgg cgatattctg aaaaaagaga caacgataac
       61 ctctttttta aatatgccga cgttatcatt gagccgaacc gaatcgagta tgttgcgaat
      121 gtggatggca ggtcagggaa cgattcaaat ctctgaccaa atgaatatca aagccaagac
      181 cgtttcatcg cataaaggta atattaaacg taagatcaaa acgcataata aacaggttat
      241 ctaccatgtc gtccgactga cggataatgt gactaatggt atttttgtca acatgcgcta
      301 acacattctg actggtggtt tcccaccagt caggctgaat aagattactc tgctttctcc
      361 acaaagatac cgtcctgatg ccctgcttca ttaaagaacc agatgccgag cgggtagtct
      421 tccagcgaaa ccaggtacat tgtgccttca ctaaactcct caactgccag taccacgcca
      481 ggacgacgcg gaccgccatc cgttttgact gttacccgat cattcacctt catcgttttc
      541 ctcctgtggc tttgtgccag tgtagaacaa tttcgttttt ctggcagcgc caggcgcgcg
      601 cgagtgctga ttttctcgac ggtctatact taagagatgc caggcggact taacgactgg
      661 cggcaacaac agagtaacgg ttgcgaggaa agatgatgaa aaccgcaaaa gagtacagcg
      721 ataccgcaaa acgtgaggtc agcgtcgatg tcgatgccct gctggcggcg atcaatgaaa
      781 ttagcgaaag cgaagttcat cgcagccaga acgattctga acacgttagc gtcgatggac
      841 gtgaatatca tacatggcgt gaattggcgg atgccttcga actggatatt catgacttca
      901 gcgtctctga agtgaatcgt tgaatgcaca ataaaaaaat cccgaccctg agggggtcgg
      961 gatgaaactt gcttaagcaa gaagcactta aaaaattcgt tacaccagga aatctgatgt
     1021 gttcatcacc ttatccgcaa tttttttcgc tgacaagaaa atattcgcga tatgaatgat
     1081 taacgctact tatgaataga aatgtgacgt gcgtcacccc ttggtggctc tggcgtcaac
     1141 gggatcgctc ccgaaaaaat catctttgat tatttgtgct gttttgagaa tcatcctggt
     1201 aaggggagaa ctgcgcgacc atgttttcaa ttcaacaacc actactggtt tttagcgatc
     1261 ttgatggcac cctgctggac agtcatagtt atgactggca accggctgcc ccctggctca
     1321 gccgtttacg cgaagcgaat gttcccgtca ttctctgtag cagtaaaact tcagcggaaa
     1381 tgctgtactt gcaaaaaacg ttggggctac aagggttacc gctgattgcc gaaaatggcg
     1441 cagtgatcca gcttgctgaa caatggcagg atatagacgg ttttccacgc atcatctcag
     1501 gtattagcca tggcgaaatc agccaggttt taaatacgct acgcgagaaa gaacatttta
     1561 aattcacaac ttttgatgat gtcgacgatg caaccatcgc cgaatggacg ggattaagcc
     1621 gtagccaggc ggcgctgacg cagctgcatg aggcgtcggt aacgctaatc tggcgcgaca
     1681 gtgacgagcg tatggcacaa tttaccgctc gtctgaacga actgggctta cagtttatgc
     1741 agggtgcgcg cttctggcac gtcctggatg cctctgccgg aaaagatcag gctgccaact
     1801 ggattatcgc gacctatcaa caattgtcag gcaaacgccc aaccacactt ggcctgggcg
     1861 atgggccaaa cgatgcgccc ttactggagg taatggatta cgcggtgatt gtgaaagggc
     1921 taaaccgtga aggggtgcat ctgcatgatg aggatccggc ccacgtctgg cgaacgcagc
     1981 gtgaaggacc ggaaggatgg cgtgaagggc tggaccattt tttctccgcc cgttaagcgt
     2041 tatcgctcgc gcatacccga ttacgcccag cctgtttagc gagataaagc cgacggtcgg
     2101 ccagtgactg gagttgttca aaatcataat caccggtttc ctcgctgcta cttaccccca
     2161 acgaggcact gatgcgtatc gtcgtactct tagcaatcaa catctctttt tcatttaact
     2221 tcaggcgaat acgttctgcg acttccgcag cctccgtcag actcgcgcct ggcagaatca
     2281 cacaaaactc ctcaccaccg acccgcccgg caacgtcctg cgcacgcaag gaactgctaa
     2341 ttaatccggc agcatgagaa agaacacggt cgcccgcctg atgaccaaag cggtcattaa
     2401 tcgctttaaa atggtcaaga tcgacctgaa tgacagaaaa aggatgttgg tgcgtctgac
     2461 acaatttagc gagcggacgg gctttttcga acagtgcgcc acgattatat aaacgggtta
     2521 aggtgtcgtg ccacgcctgc cactgcaacg agctttgcag aacatacatg ttgctgacca
     2581 tccggcgaat cacataccag gagatgagta acatggtggt aaagagcgcc cacagcaggg
     2641 ttaatgcaat gctgatacta ccgaaatcgc cgcgcacgcc ttcgcttagc gtatggacgc
     2701 gcaccagcac accgtcgaaa tggtccagac gttcccagct aacatagcga ctgtccatac
     2761 gaatgccgcc ccgcgtgtca tgttccatcg cctgcgccag caaggccagt tcacgaggat
     2821 caaaaatatt ccctgttgga tggtcaggat tggaagaggt caaaaatctc agcttactgt
     2881 catagagctg atactcacca tcgaggtttt tatcgatggc gtttcttaaa aattgctgca
     2941 tagtacgtac gggaatactc atccccagca cgccatacca gtagttatta ctgtctaccg
     3001 gaacactgac ggtaacctgc ggttcagtat tgctggcgtg ttccggttgc gaagtaaacc
     3061 agcgtaccgc gcggtgacga ttttctcgtt gcgaatggcc gataaaccag ggttgggtga
     3121 catagccgta ataacgcgtt ggaacattgc gcgtaaacaa ggtcggctgc gtcgaaacgt
     3181 aaaatccggc acgcgagaca tacatcgcct gttcaaccat cgacgaggag ttgtgcgcca
     3241 gtcgcagcaa gtaaccaact tccagtgcag cggtaatttc attgtcgagg ctttcatttt
     3301 cgcgagacag gagattcccc tcgctgacta atgcatccga cacaccatta actggcagag
     3361 tgcgccgtcg gttgagttca atttgccagg cgtgctcgtc gcgatgctgt tcgaactcgg
     3421 ttacagcatt acgcagagag gtgaaatcca gtggcgctac gagagcttcg cgcatgccat
     3481 tacgcaaaaa gatcagtttg tcgacgttat actgcaaatg cttatcgagc gcgttggcta
     3541 cgttttccag atgattacgc tggctggaga tataggcatc ttccagcacc actacttcac
     3601 gccaggtgag caaggtggaa aaaagcaata ccacgataaa gcagagatta acgacatgac
     3661 caggccccag gcggcgtgcg agttttttca accagctctg gttttccatt tttgtctcgt
     3721 gctgcaccct gacgactccc aacctgcttt tttatgattc tggcataacc tggcgatagc
     3781 gtaccgcaaa acagcgataa ttttataatg gtggcatgat aagacgcgac tatcattcat
     3841 tgcaggcatt ggcacaccag gccggatgtg atccggcctg gtgttaatga taccctttca
     3901 aacttcttcc gcagaacgac tcccctcccc cggaacaagc tcggtttcat gaaaagcttc
     3961 ccgcttgacc ccgtaaccgt cataccaacg acactcaacc ataccgctgg agtatccggt
     4021 gacaatcatc cgcgggccgc cctctttaac cgtaacttcc tcactaacca taaagctcat
     4081 actcgcctcc ttttttcgag tgaaacttgt tcaccttagt tgaagatggc gaattttgca
     4141 taatagcctg tgcgagtatt aatgtgcctg accgcgtatt ttcgctacgg ctttcactcc
     4201 cagcaccacg atgccgccga tgataaatcc aagaatcaga tttaaaacag tcggtaatat
     4261 catcgccacc actgcacttt gctgcccggc gaaatgttca atggcgtgat gcagcggcgc
     4321 aattccatgt accacaatcc caccgccgac gaggaacatc gccagcgtgc cgacaatcga
     4381 taacgccttc atcaaccagg gcgcaatgat caataaccct ttacctaatg cctgcatcag
     4441 cgcgctggat ttttccgcca gccaataccc caggtcatca atcttaacga taacccctac
     4501 cagaccgtag acgcccacgg tcactaccag cgcgatgcct gaaagcacca gcacctgatt
     4561 aagcaacggc gcttcggcca caatccccag cgtgatggcg acgatttcgg cagacaatat
     4621 aaaatcggta cgaatcgccc cttttatttt gtccttttca aacttcagcg gatcctgcgc
     4681 cgccagcttc tccagacgct gctggctctg cgccggatct tctttatgtt tacgcgcctc
     4741 cagcatatgc agcactttct ctactccttc aaagcagaga aacgcgccac caatcatcaa
     4801 cagaggcgta atcgcccacg ggataaacgc actgatgatc agcgccagcg gcaccagaat
     4861 cactttatta atcagcgatc ctttcgccac gccccagacc acgggaagtt cccggttggc
     4921 ccgcacgcct gaaacttgtt gcgcattgag cgataagtca tcccctaata caccggcggt
     4981 tttcttcgcc gccagtttgc ccatcacgga gatatcgtcc agtagtgtgg cgatatcatc
     5041 gagcaacgtc agtaaactac tcccggccaa aagcattccc tctcattttt attgttgaag
     5101 ggtgtaagta tgaagcaaaa acggcagctc tgatacttgc ttcacaggtc aacatttttt
     5161 taacaatcaa gtgcaattat atctctcgcc agcagaatga ttttcacgtt tactatgaac
     5221 caccttttta tttccaccgt gagggattat gcgtttccgc cagttgttac cgctttttgg
     5281 cgcgctgttt gcgttgtata tcatttgggg ctcaacctat tttgtcattc ggattggcgt
     5341 ggaaagctgg cctccgttaa tgatggcagg cgttcgattc ctggcagccg gtattttatt
     5401 gatggcattt ttgctactgc gcggacacaa gctccccccg ctacgtccgc tgctcaatgc
     5461 cgcgctgatt ggcctgttat tgctggctgt cggtaatggc atggtgacgg ttgccgagca
     5521 tcaaaatgtt ccttccggca tcgccgccgt tgtggttgca accgtgcccc tctttaccct
     5581 gtgcttcagc cgcctgtttg gcattaaaac gcgcaaactg gaatgggtgg gtatcgccat
     5641 tgggcttgcc ggaatcatca tgctcaatag cggtggaaat ttaagcggca atccgtgggg
     5701 cgcgattctg attttaatcg gctcgattag ctgggcgttt ggctcggttt atggctcgcg
     5761 cattacctta cctgtaggga tgatggcggg tgcgattgag atgctggcgg caggcgtggt
     5821 gttaatgatc gcgtcgatga ttgcaggtga aaaactgacg gcgctccctt ccctttcagg
     5881 cttccttgcg gtcggctatc tggcgctgtt tggttcgatt atcgccatca acgcttatat
     5941 gtatttaatc cgtaatgtca gtccggctct cgccaccagc tacgcctacg ttaacccggt
     6001 ggtcgcggtc ttgctgggta cgggactggg cggagagacg ctgtcgaaga ttgaatggct
     6061 ggcgctcggc gtaattgtct tcgcggtggt actggtcacg ttgggaaaat atctcttccc
     6121 ggcaaaaccc gtagttgcgc cagttattca ggacgcatca agcgagtaaa tgaatcccct
     6181 gcgtgtcgat ctgcgcgctg gcaccttcgc cgcagatcca ctcttccaga cgctcggtaa
     6241 gcgcttcatc cgtcagcttc tcgcgcccac gtaatgcaca ctcccagaca atcagtacgc
     6301 gccagccgag ttcctgcaaa cgactgatat cgcggcgatc gcgttcaaca tttttaccta
     6361 tcttctccag ccagaactcg gttcgagtcg caggcacttt aaacagatag cagtgatgat
     6421 gatgccagaa gcagccatgg gtaaatatca cgcagcgata ttcatcaacg acaaaatccg
     6481 ggcgaccggg cagactggcg tcctgaacgc gaaacgccag gccctgcccg gttaacagac
     6541 tggcgaggcg cttctcaatc gccgtatcac gcgtggcaat cgcgcgcata tttttgctgc
     6601 gagtggcctt atcgtgaacg tcggccatgt tgtgcctctt gctgacgcaa cgccaccgcc
     6661 tgtttgattt ttggctcaag cagttttgcc accgcggcaa agaccggcac gaccaccgag
     6721 ttaccgaact ggcgataggc ctgagtgtcc gaaaccggaa tgcggaattt cgcttctccc
     6781 ggcgcttcaa aacccattaa gcgcgcgcat tcacgaggcg ttaaccgacg tggacgatgt
     6841 tgctgattct gcggatcgtc aaagtctttc tcacctttgg ccatatccca gccgcgatcg
     6901 attaaaattt ccgcgccatc tttgtaataa cgcgcagaca gcgtacgggt gacgctttgc
     6961 ggattgttcg gataaaccat tccataaccg aagccgttac cgcgcgcctg atgttttttc
     7021 gcatatcgat agaggtactt ccacagcacc ggcgtcagga tatatttcgc ctcgaccatc
     7081 gggtccaaca gctgcgccag cgtcactcgc tgtgcaggga aacattcgct gatatcacgc
     7141 agagtaaaat cggctttcag attaagatcg cgacgaaaac ccaccagcac gatgcgttca
     7201 cggtgctgcg gcagaaaatg tttaccatcg atgattttcg gatcgtccgg cccgttatct
     7261 tctgcatcag ccacgtcata gcccagttcg tccagcgtct gcatgatgat gcggaacgtt
     7321 ttaccctggt cgtgactttt caggtttttg acgttttcga gcacaaacat cgccggacga
     7381 cgcgcgtcga taatgcgtac cacatcaaaa aacagcgtgc cctgggtatc gcaggcaaaa
     7441 ccgtgcgccc gcccgagcga gttctttttc gatacgccag ccagcgaaaa tggctgacaa
     7501 gggaaaccgg ccagtaaaac atcgtgttca ggaatgtgtt gacgaatatg ttccgccgcc
     7561 gcctcatcac tcacgccttc tttatggctg agggtgatat cgcggatatc ttcattaaaa
     7621 tgatgcgtcg ccgggtcgca ataatggttg gctttataag tgcgtaccgc atgtttgttc
     7681 cattcgctgg taaacacgca ctgtccgcca atcgattcaa aaccgcgacg gatgccgcca
     7741 attccggcga atagatcgat aaagcgaaac gcataatgcg gatgatgtgc cggtggtttg
     7801 ggtaacaacg tttgcagatg ggcgaactct ttctcactta aacggtgcca tgccgagtca
     7861 ttcgccagcg cacgttttaa aattgccgcg ctccagtgat tctcacctac accattaagc
     7921 tgcgccacca gcgttttaac atcataaatt tgcagcagtt tctccagcat tgcctgtgcg
     7981 gcattcccgg tgctgtatga atcggttact gatatatttt cctgcataga tttaaccggc
     8041 catctgagat ggccgacagg ttaacataat tacagccatt tagggccacg cgctcagccc
     8101 gcggatgaaa acgcatctat caccttgtga tcgacacctt cattctcccc cgccagttcg
     8161 gcactgagct tcgccataaa ctcgactaaa aaatgcgcgt tgtgctgcgc cagctgcttg
     8221 cctcttacgg tttgcatggt ttgcggtagc ttcagcaact tagtctggaa atgatccagc
     8281 gcgtagcgtt tatcatccag cgggcgatgc tgtgcgagcg gatcttcgcc atcaaacagc
     8341 gctacgccca atgcccctga aacggcaaac acgcgcgcca ggccaatcgc tcccagggct
     8401 tccagccgat ccgcatcctg gacgatttta gcttccgttg ttaagggggc gatttgcgcg
     8461 ctgaaactgt gagcggcaat ggcatgacaa acggcctcga ttttctccgc cggaaattgc
     8521 gcgaactctt cacggagcag gcgacgcgtc tcttctgccg ccaggattga agaacgctgc
     8581 ctttgcggat gatttttcgc caggctcaca atatcgtgaa aataacaggc agttaaaatc
     8641 accagcatat cgacatcgtc atctgccgcc agcttctgtg ccgtagccca gacgcggcga
     8701 aaatgacaca cgtcatgggc cgcgtcctgg tgctgatgat ggtttttcaa ccagttttcg
     8761 aactgtgcct gccagtgttg taagtccata cgcgctccgt tgccaaagag tcgccacctt
     8821 agcagttttt attcatagcg tctgctacgt gcgggacgag gtcgggtgta ccaggcaatg
     8881 ctgcctaaca atgccccgac cagagccata aataaaaatc cgaccagtgc ccctaaccac
     8941 tttcccgcgg tagagcctaa atcgctttca aatcccgaca cgggggcatc ggggatccgg
     9001 ctcatcaccc agatgtaacg cgccattgcc cagacgaaat agccacacca tgtatagaaa
     9061 gcccacaaag ccagtttgcc gccagggctg cgagagagtc tctcttccat ctttgtcgaa
     9121 ccgtattcca ttttgagata acccggaaaa ttcagactta gtactattat gtgataaaag
     9181 tcacattttt ccacattgga tgagccagtc aagactcaat cattttttga acaaatagat
     9241 aaattttgcg caacggcagt gaatacagga aaaatcgttt tgagcgattt tatttatctt
     9301 tgatatcggc tttattaatt catttaatca atatattagc actgattaca attatacctt
     9361 tttattattc tgtgtcatga tgcttccgtt attagccttt tatcgtcttg tttatatttt
     9421 ttgggccggc atgatgccgg ctttttttat gccttcatta atgtgcgcct gatcacacca
     9481 gccgtttggc gcaacaatca ttgatacccc ctatgtttcc gctcaactca taacacattg
     9541 atatacatt
//
LOCUS       DAZKIV010000162         9518 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZKIV010000162 DAZKIV010000000
VERSION     DAZKIV010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.162"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..263
                     /gene="yafQ"
                     /locus_tag="ACHOT6_003990"
     CDS             <1..263
                     /gene="yafQ"
                     /locus_tag="ACHOT6_003990"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFP9949134.1"
                     /translation="EYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDH
                     PLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            419..1159
                     /gene="dpaA"
                     /locus_tag="ACHOT6_003991"
     CDS             419..1159
                     /gene="dpaA"
                     /locus_tag="ACHOT6_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFP9949135.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1130..1897)
                     /locus_tag="ACHOT6_003992"
     CDS             complement(1130..1897)
                     /locus_tag="ACHOT6_003992"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFP9949136.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKLLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2103..2681)
                     /gene="lpcA"
                     /locus_tag="ACHOT6_003993"
     CDS             complement(2103..2681)
                     /gene="lpcA"
                     /locus_tag="ACHOT6_003993"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFP9949137.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2921..5365
                     /gene="fadE"
                     /locus_tag="ACHOT6_003994"
     CDS             2921..5365
                     /gene="fadE"
                     /locus_tag="ACHOT6_003994"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFP9949138.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDIFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5408..5854)
                     /gene="ivy"
                     /locus_tag="ACHOT6_003995"
     CDS             complement(5408..5854)
                     /gene="ivy"
                     /locus_tag="ACHOT6_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFP9949139.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCCSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            6035..6802
                     /gene="yafV"
                     /locus_tag="ACHOT6_003996"
     CDS             6035..6802
                     /gene="yafV"
                     /locus_tag="ACHOT6_003996"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFP9949140.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            complement(7026..8162)
                     /locus_tag="ACHOT6_003997"
                     /pseudo
     CDS             complement(7026..8162)
                     /locus_tag="ACHOT6_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(8265..9391)
                     /locus_tag="ACHOT6_003998"
                     /pseudo
     CDS             complement(8265..9391)
                     /locus_tag="ACHOT6_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2332 a         2348 c         2270 g         2568 t
ORIGIN      
        1 ttgaatactc gggacaattt tcaaaggatg taaaacttgc acaaaagcgt cataaggata
       61 tgaataaatt gaaatatctt atgacgcttc ttatcaataa tgctttaccg cttccagctg
      121 tttataaaga ccacccgctg caaggttcat ggaaaggtta tcgcgatgct catgtcgaac
      181 cggactggat cctgatttac aaacttaccg ataaactttt acgatttgag agaactggaa
      241 ctcacgcggc gctctttggg taaagcacgc tgccgatgaa atttgtaaac cctgcgccga
      301 caggcttgcg taaaactctt ttggtgataa tgtgagcctt ccaaatccag actattccga
      361 tttgggatcc attttgccgt cctggcgtgt gtaaccgttt tatcaaggaa taagcagtat
      421 gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg tgcgtttcgt ttgccggttt
      481 gctgggcagc agtagctcga ccacgccagt gagcaaagag tataagcagc agttgatggg
      541 atcccctgtc tacatccaga tcttcaagga agaacgtacg ctcgatctct acgtcaaaat
      601 gggcgagcaa tatcagctgc tcgacagtta taaaatctgt aaatattccg gcggattagg
      661 gccaaaacag cgtcagggtg atttcaaaag cccggaaggg ttttatagcg tccagcgtaa
      721 tcagttaaaa ccagacagcc gttactacaa agcgattaat attggtttcc ccaatgccta
      781 tgaccgtgcg catggttatg aagggaaata tctgatgatc cacggcgatt gtgtttccat
      841 cggctgctac gcaatgacca atcagggtat tgatgagatc ttccagttcg ttactggtgc
      901 gctggtgttt ggtcagccga gcgtgcaagt gagtatttac ccgttccgca tgaccgacgc
      961 caatatgaag cgccataagt attccaactt taaggacttc tgggagcaac tgaaaccggg
     1021 ctacgactac tttgagcaaa cccgtaagcc accgaccgtt tctgtggtca atggccgtta
     1081 cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca caactggcat caaactacac
     1141 gctccccgag gcaaaataag cgccattcgc ctggcataat cttttgccag gtttcattgc
     1201 ccgtcagcgg ctgtgttgca atcaccgtga ccacatcatt cggtgtggtc tgcgagctga
     1261 agtcgatttc cacatcctga tccagcaacg ttgccacgcc aaacggcgcg cggcgggtga
     1321 tccagtgtaa attagtcgag caatacgcca ttacatagcg cccgtcagaa agcagcatat
     1381 tgaaaacgcc cttctgccgc agttcatccg ccagtgaggc gatatattta aacactgccg
     1441 ccatgttgcc cggtgtacgc gggtaacgct gcgttaattt atgcagcagc cagcagaagg
     1501 ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc ggtttccagt aatttgtagc
     1561 ccgtcagttg tccgttatgg gcataagtcc agttacgccc ccataactcg cgggtaaatg
     1621 ggtgagtatt ttccagcgcc acctcgcccc gattagcctg gcgaatatga gccaccaccg
     1681 aacaggattt tatcgggtag tcctggacaa gtttggcgat gggggaatta aagctgggtt
     1741 gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa ggtaatgccc cagccatctt
     1801 tatgtggccc ggttccacca ccacgctgta caagtccggt gaaactaaag cagatatcgg
     1861 tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg taacctccac aacagcggga
     1921 gcgcatggcc cccggcgaag attgatttaa gaattttcag gtcggatgcg gcgtaaacga
     1981 attattcggc ctacggttcg cgtacagatt tgtaggcatg ataagacgcg tcagcatcgc
     2041 atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg ccttatccgg cctacgccag
     2101 acttacttaa ccatctcttt ttcaattaac tggatcagga tatggatcac tttaatgtga
     2161 atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc gaatttcgat atccgccgtg
     2221 ccagccattt tgccgccgtc tttaccggtc agggtgatca ctttcatccc cttctcacgc
     2281 gccgctgcga tcgctttgat cacgtttgca gagttaccgg aggtggagat ccccagcagt
     2341 acatcgcctt cgcgacctac cgcttcaacg tagcgggaga aaatatcatt gaaaccgaaa
     2401 tcattaccga cgcaggaaat atgactaacg tcagaaatag caatcgccgg gtagcccgga
     2461 cggttttcac ggtagcgacc ggtcaactct tcggcaaagt gcatagcgtc gcaatgggaa
     2521 ccgccgttgc cgcaggaaag cactttgcca ccggctttaa agctgtctgc taacaggacc
     2581 gccgcgcgct gaatggcgtg aatattggcg tcatctttta aaaagttagc cagcgtttcc
     2641 gccgcttcgt tcagttcgtt acgaataaga tcctggtaca tgaggatatc cttcagcata
     2701 aatgtaatag acaaaatgca gtgtaccgga taccgccaaa agcgagaagt acgggcaggt
     2761 gctatgacca ggactttttg acctgaagtg cggataaaaa cagcaacaat gtgagctttg
     2821 ttgtaattat attgtaaaca cattgctaaa tgtttttaca tccactacaa ccatatcatc
     2881 acaagtggtc agacctccta caagtaaggg gcttttcgtt atgatgattt tgagtattct
     2941 cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc gtgagcttat ttatcagcag
     3001 tctgattttg ctcgcctgga cagccgccct cggcgttgct ggtctgtggt cggcgtgggt
     3061 actggtgcct ctggccatta tcctcgtgcc atttaacttt gcgcctatgc gtaagtcgat
     3121 gatttccgcg ccggtatttc gcggtttccg taaggtgatg ccgccgatgt cgcgcactga
     3181 gaaagaagcg attgatgcgg gcaccacctg gtgggagggc gacttgttcc agggcaagcc
     3241 ggactggaaa aagctgcata actatccgca gccgcgcctg accgccgaag agcaagcgtt
     3301 tctcgacggc ccggtagaag aagcctgccg gatggcgaat gatttccaga tcacccatga
     3361 gctggcggat ctgccgccgg agttgtgggc gtaccttaaa gagcatcgtt tcttcgcgat
     3421 gatcatcaaa aaagagtacg gcgggctgga gttctcggct tatgcccagt ctcgcgtgct
     3481 gcaaaaactc tccggcgtga gcgggatcct ggcgattacc gtcggcgtgc caaactcatt
     3541 aggcccgggc gaactgttgc aacattacgg cactgacgag cagaaagatc actatctgcc
     3601 gcgtctggcg cgtggtcagg agatcccctg ctttgcactg accagcccgg aagcgggttc
     3661 cgatgcgggc gcgattccgg acaccgggat tgtctgcatg ggcgaatggc agggccagca
     3721 ggtgctgggg atgcgtctga cctggaacaa acgctacatt acgctggcac cgattgcgac
     3781 tgtgcttggg ctggcgttta aactctccga cccggaaaag ctgctgggtg gcgcagaaga
     3841 tttaggtatt acctgtgcgc tgatcccgac caccacgccg ggcgtggaaa ttggtcgtcg
     3901 ccacttcccg ctgaacgtac cgttccagaa cggaccgacg cgcggtaaag atatcttcgt
     3961 accgatcgat tacattatcg gcggaccaaa aatggccggg caaggctggc ggatgctggt
     4021 ggagtgcctc tcggtaggcc gcggcatcac cctgccttcc aactcaaccg gcggcgtgaa
     4081 atcggtagcg ctggcaacgg gcgcgtatgc tcacattcgc cgtcagttca aaatctctat
     4141 cggtaagatg gaagggattg aagagccgct ggcgcgtatt gccggtaatg cctacgtgat
     4201 ggatgctgcg gcatcgctga ttacctacgg cattatgctc ggcgaaaaac ctgccgtgct
     4261 gtcggctatc gttaagtatc actgtaccca ccgcgggcag cagtcgatta ttgatgcgat
     4321 ggatatcact ggtggcaaag gcattatgct cgggcagagc aacttcctgg cgcgtgctta
     4381 ccagggcgca ccgattgcca tcaccgttga aggggccaac attctgaccc gcagcatgat
     4441 gatcttcgga caaggagcga ttcgttgcca tccgtacgtg ctggaagaga tggaagcagc
     4501 gaagaacaat gacgtcaacg cgttcgataa actgttgttc aaacatatcg gtcacgtcgg
     4561 tagcaacaaa gttcgcagct tctggctggg cctgacgcgc ggtttaacca gcagcacgcc
     4621 aaccggcgat gccactaaac gctactatca gcacctgaac cgcctgagcg ccaacctcgc
     4681 cctgctttct gatgtctcga tggctgtgct gggcggcagc ctgaaacgtc gtgagcgcat
     4741 ctcggcacgt ctgggggata ttttaagcca gctttacctc gcctctgccg ttctgaagcg
     4801 ttatgacgac gaaggccgta atgaagccga cctgccgctg gtgcactggg gcgtacaaga
     4861 tgcgctgtat caggctgaac aggcgatgga tgatttactg caaaacttcc cgaaccgcgt
     4921 ggttgccggg ctgctgaatg tggtgatctt cccgaccgga cgtcattatc tggcaccttc
     4981 tgacaagctg gatcataaag tggcgaagat tttacaagtg ccgaacgcca cccgttcccg
     5041 cattggtcgc ggtcagtacc tgacgccgag cgagcataat ccggttggct tgctggaaga
     5101 ggcgctggtg gatgtgattg ccgccgaccc aattcatcag cggatctgta aagagctggg
     5161 taaaaacctg ccgtttaccc gtctggatga actggcgcac aacgcgctgg cgaaggggct
     5221 gattgataaa gatgaagccg ctattctggt gaaagctgaa gaaagccgtc tgcgcagtat
     5281 taacgttgat gactttgatc cggaagagct ggcgacgaag ccggtaaagt tgccggagaa
     5341 agtgcggaaa gttgaagccg cgtaattgaa taacggagcc gaaaggctcc gtttctttat
     5401 ccgctaatta tttaaaatta aagccatccg gatggttttc caggctgccg gtcaacgccg
     5461 cgaacagcac cgttttacca tcaatcgaaa gcgcatcgtt cacattcaac caggtgagtt
     5521 tctcttgcga cgttttctca tcaatagtcg agaacagccc cgtcatctga ttagatttct
     5581 cggaccacat cacagcgata cgttgcgagc aacagtcatg cggtttgcac gcgctcatca
     5641 cctgatacgt ctcatctccc aacgttacgg tttgtgcggg agtataagta ccgcctttca
     5701 tcacccaggc aggcaactta tgcccttgta ccatctgatt aaatgcagct ttggtggttt
     5761 cgccctttgc aaggctgcta atggttaaat catcctgcgc cattgcactg gtggcgatga
     5821 ccagagcggc gactgtcgtt attgccttaa acatcattcc tcccgagctt atcctgccca
     5881 tgttattaac cttctaaagc ctggcgataa cacgataatt tgcaaatcag atatttccaa
     5941 tatcagcatt tgttatcaat ttattctgcg ctaaggcaac tgcctgataa ggtgttaaag
     6001 ttataaaacc cttcgcataa aggagttgta caccgtgcct ggtttgaaga ttacgctttt
     6061 gcagcaacca ctggtgtgga tggatggccc tgccaacctg cgtcattttg atcgtcaact
     6121 ggaaggtatt accgggcgcg atgtgatcgt tctaccggag atgtttacca gcggctttgc
     6181 catggaagcg gcagcttcgt cgctagcaca agatgacgta gtgaactgga tgacagctaa
     6241 ggcgcagcag tgcaatgcgc tgattgcagg cagtgttgca ttacaaacgg agtctggttc
     6301 ggttaaccgc tttttgctgg ttgagccggg cggcacggta catttttatg ataagcgtca
     6361 tctgttccgc atggcagatg agcatctaca ttataaagcg ggcaatgcgc gagtgattgt
     6421 ggaatggcgc ggctggcgta ttttgccgct ggtgtgctac gacttacgtt ttcctgtgtg
     6481 gtcgcgcaat ctcaacgatt atgacctcgc cctgtacgtc gccaactggc ctgctccgcg
     6541 ctctctgcac tggcaggcat tgctgacggc ccgcgcgatt gagaatcagg cgtatgtggc
     6601 gggatgcaat cgcgtcggca gcgatggcaa cggctgccat tatcgcggtg acagccgggt
     6661 gattaatcca caaggcgaga ttatcgctac tgccgacgcg catcaggcaa cgcgcattga
     6721 tgccgagctg tcgatggcgg tgttaaggga atatcgcgat aagtttccgg catggcggga
     6781 tgcggatgag tttaggttgt agtgagtggg ggctgtattt agggcgggga attcagaaag
     6841 ggatgtttag tgtgctgagc gagagttaaa taataaaaaa ctgggaagat ttaataatcc
     6901 ccccgcaggg caagagcatg aattaaattt gtgttaaatt tttcatttta tcagttcgaa
     6961 acccaagctc tgagcaggat gcaagcactt cttctatata acatgtgttt aacgccaggg
     7021 caagattacg aaagcccgct ccccgcaagg actgacgcca gatagtttct gtccatggct
     7081 gcttttcgca tcttacgtct taaccctgcc ttgaatacct tatcattcgt caaaatatta
     7141 atagcaatgt gccgtatccc tgaaaataat tctgctgcat ttcctcttct tattttgcag
     7201 tcgtcttcat tcattaccac gtccagacgc cagtgcagct tattctccac gtgccagtga
     7261 tttcggatcg ctgtggcgaa tttctcagcg gttaaatcag cagaactgat ataatatctg
     7321 accgtcattt ctggctcttt cttttgttct gctattatgg accgaaagga gactgccacg
     7381 cataatttct tcagcccttt ccattcaaac gtgaaatcaa taagttcatc agggacatcg
     7441 caaacaatat gaagacggat ttcttctctg ccgtgactct tttcactaat tgcgtaactg
     7501 tcatgctctg gattatttaa ttctttcagc ggaaattttt cctcaaaggc tttatttagc
     7561 cgcccctggt ttccttttac cgcgaataaa taatcacctc cctgtttttg tatcttctct
     7621 gcaatatctt tctggcaacc catcgcatca gttgtgatga tttttccttt aatatccagc
     7681 atgttaagaa gttctgggat agctgtaatc tcattagatt tctcatccgt ctcgatctgt
     7741 ccgatgacca gactgtgcat tgttgagaac gcactaatga catgaatcgc tcccctgcgg
     7801 cgactcttgt cataagagtg ccggagcgtt tttccatcaa ttgcgatgac gtctttatca
     7861 tctgaagaat ggcagtcacg catccagtta ataaagcact catgaaattt tgcaggactg
     7921 atacaggata caactctggc aatagtatcg tgaacaggaa tatcattttc aaaatcacca
     7981 tattgcttca agaaatcgag atgtgtttcc ccgaaatcct ctatatcttc ccaactttct
     8041 gcaccagaaa taacggcaaa aatagtcaat agtaggatgc ctgacaattt atgctctact
     8101 ttccaggctt atctgtaatc gggaataata gaaatatgtt ccatcaattt tttaagttcc
     8161 atttgttcac cttatttatg taaggagtat ttgatcatgt ataagcaata aaaaacagct
     8221 tcaggtaatg aggaatatct caatttttaa acataaaatg cgaattattt agtacaaaaa
     8281 gtgaacttct gatgatcttt ccctgtgttt aacgcagcct tctttctttt cctcttcacc
     8341 cttaatgtct ttgcaatctc ttaataaatt cagtgccatc ttctttattc cagatattat
     8401 ttaggctgcg tttcctcttc ttatccggct ggcatcttca ttcattttta catctaacac
     8461 ccaatgaaga ctgtgctcga tcagccagtg cgctctgata gcatgtgcaa attctttagc
     8521 atccatatcc tttgatgaaa tataatatcg gatgcttaca ccttctgctg atttatcttc
     8581 tttcttctgc ctgaatgaca atgctacaca aagctttttt aatcccttcc attcgaattc
     8641 aaaatcacaa aagttcaggc gttacgttac tgacaatatg caaacgtgtt tcttttcttc
     8701 catgacttat ctcctgcgta ctaaacgaat cgcctttata attagaaaac acatttacag
     8761 gaaatttttc ctcgaatgca tgatgtaatt tcccctgatt gccttttact gccagaagat
     8821 aatctgcttt tttatctttg atcttcgaag cgatatcttt ctgacagccc atagcatcaa
     8881 tggttataaa atttttcttt aaatacagta ggttaagcaa ctctgaaatg gctgtaatct
     8941 cattactttt ggcttccgtt ttcacctgcc ccagtacaac accattttcg ttcgagaatg
     9001 cactcaccat atggattgct ccttttcttt ttcccttatc aaaggagcct cttatggtct
     9061 ttccatctat tgctataatt tcaccatcag tgatttcatg gcactcctgc atccattcaa
     9121 taaacatctt ttcaaaggcc aaactgtcaa tgttactcac aacgcgtgca atggtgtcat
     9181 cgaccggaat gccattatca aaatcaccat atttctttag ccattcaagt ctttcatgtc
     9241 caaaatcttc aatttcctgc cactcatcgg cacctgcaat tactgcacat acggtgagaa
     9301 acaaaatagc agataattta tgtttaacct ttccttgttg tcgtatatca gggatcactg
     9361 aaatataatc aagcaaactt tgaatactca cctggcgtcc ttttttatct ttttagttat
     9421 tccaaaggtt aaattttgat cacctgtagg cgttaaagcc aagatgaaaa tcgtgattta
     9481 catcacaatt ttcatgatct tgccctgccc cgtccctt
//
LOCUS       DAZKIV010000163         9434 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZKIV010000163 DAZKIV010000000
VERSION     DAZKIV010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.163"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(67..291)
                     /locus_tag="ACHOT6_003999"
     CDS             complement(67..291)
                     /locus_tag="ACHOT6_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9949141.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQRTCSDRFT"
     gene            683..1192
                     /locus_tag="ACHOT6_004000"
     CDS             683..1192
                     /locus_tag="ACHOT6_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9949142.1"
                     /translation="MATQTEVARHLSLTDRQLRRLQKLPGAPISNKRGQLDLDAWRDF
                     YISYLRRSKNDVPDGDSEDDYEEKLLIARWELTAEQAVTQQLKNEVSKGKLIDTGFCI
                     FALSKLAMALSSTLDSIPLSMQRQFPDLTPRHLDHLKTLIAKGANQCARAGDKLPDLL
                     DEYIRATTE"
     gene            1164..3092
                     /locus_tag="ACHOT6_004001"
     CDS             1164..3092
                     /locus_tag="ACHOT6_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9949143.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEAQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPSR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHCVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVLAKSREEETTRP
                     TLKELAAKLSGGVNGYSR"
     gene            3076..3282
                     /locus_tag="ACHOT6_004002"
     CDS             3076..3282
                     /locus_tag="ACHOT6_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFP9949144.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELN
                     RAINDAESVLGTTRRRRRPLGVRL"
     gene            3279..4871
                     /locus_tag="ACHOT6_004003"
     CDS             3279..4871
                     /locus_tag="ACHOT6_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9949145.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     LFRTRFKAVSPKRVDTPGHGMGNRFLRAGVEVDRYGRAVAYHICEDDFPFSGSGRWER
                     IPRELPTGRPAMLHIFEPVEDGQTRGANQFYSVMERLKMLDSLQATQLQSAIVKAMYA
                     ATIESELDTEKAFEYIAGAPQEQKDNPLINILEKFSSWYDTNSVTLGGVKIPHLFPGD
                     DLKLQTAQDSDNGFSALEQALLRYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRY
                     FMGRRKFIAARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA
                     IDGLKEVQESVMRIEAGLSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIA
                     QAYQQQIAESRRPEEETTPRET"
     gene            4861..6366
                     /locus_tag="ACHOT6_004004"
     CDS             4861..6366
                     /locus_tag="ACHOT6_004004"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9949146.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGAHKVDGN
                     QFEALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLA
                     DELVNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAAAAPQQPVASTQSEADRIMACEEANGREQLAATLAAMPE
                     MTVEKARPILAASPQADAGPSLRDQIMALDEAKGAEAQAEQLAACPGMTVESARAVLA
                     AGSGKAEPVSASTTALFERIMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            6403..6750
                     /locus_tag="ACHOT6_004005"
     CDS             6403..6750
                     /locus_tag="ACHOT6_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9949147.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGSSGISSATPALTPLMLD
                     EATGKLVVWDGQKAGSAVGILVLPLEGTETVLTYYKSGTFATEAIRWPDSVDEHKKAN
                     AFAGSALSHAALP"
     gene            6808..7836
                     /locus_tag="ACHOT6_004006"
     CDS             6808..7836
                     /locus_tag="ACHOT6_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9949148.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     DPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGN
                     NIEQADGKKWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLG
                     NTAADGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            7888..8271
                     /gene="gpFI"
                     /locus_tag="ACHOT6_004007"
     CDS             7888..8271
                     /gene="gpFI"
                     /locus_tag="ACHOT6_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9949149.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRKCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
     gene            8264..8617
                     /locus_tag="ACHOT6_004008"
     CDS             8264..8617
                     /locus_tag="ACHOT6_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9949150.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            8633..9166
                     /locus_tag="ACHOT6_004009"
     CDS             8633..9166
                     /locus_tag="ACHOT6_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949151.1"
                     /translation="MAIKGLDQAIDNLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLPVIKLGIRMPGRRPNSILKAGQ
                     HRYQRAFIQRLKNGRWHVMQRVAGKNRYPIDVVKIPMAAPLKQAFDENVDRIRRERLP
                     KELASALKQQLRIAIKR"
     gene            9163..>9434
                     /gene="gpU"
                     /locus_tag="ACHOT6_004010"
     CDS             9163..>9434
                     /gene="gpU"
                     /locus_tag="ACHOT6_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9949152.1"
                     /translation="MKHTDIRAAVLDALEQHEHGATLFDGRPVVFDEEDFPAIAVYLT
                     DAEYTGEELDADTWRATLHIEVFLPAQVPDSELDQWMESRIYPAMTA"
BASE COUNT         2172 a         2212 c         2895 g         2155 t
ORIGIN      
        1 gttattgcct gttaccataa gaaaaagcga ctttagtggt cgctttttgt gtcatatata
       61 agtcgtttaa gtaaacctgt ctgaacaggt tctctggtcg tgtttgtctt tgttgggtac
      121 aaattgagaa tatttttcat taattaatct tcttctgcag gcttcaataa cccacgctga
      181 aaaatttcct gaacctttca gatcaagagc gatgttaatt tgttcaatca tctggtttgg
      241 aaatcggatg ttgcgggttg ttgttctgcg ggttctgttc tttgatgaca taatgttgcc
      301 ccatattcag tgttgctgat ttgtattatc tgaagttgct tttacgttaa tttgatgcag
      361 atcaattaat acgatacctg cgtcataatt gattatttga cgtggtttga tggcgtacac
      421 gcacgttgtg acatgtagat gataattatt attttgtggg tcctttccag cgcttcgctc
      481 tgttacgggg cggcgacctc gcgggttttc gctatttatg aagattttcc gggatccatg
      541 tccggtttct cttcaagtta actatatgaa aaatataaaa acaggtcttc tgtgaaccgg
      601 acatgaacaa aaaacagaca tgtaaaccgg acatgaccgg ttttgttgtg attgtgaggt
      661 gagagttttt gcgaggtgag gagtggctac gcagactgaa gttgccaggc atttaagtct
      721 gaccgatcgc cagcttcgca gattgcagaa attgccgggt gccccgatat cgaataagcg
      781 agggcaactg gatctggatg cctggcgcga tttttacata tcgtatctga ggagaagtaa
      841 aaacgatgtg cctgatggcg atagcgaaga cgactatgag gagaaattgc ttattgccag
      901 atgggaactg acagcagaac aggctgttac acagcagtta aaaaatgagg tgtcaaaagg
      961 aaaacttatt gacaccgggt tctgtatttt tgccctcagt aagctggcaa tggcgttatc
     1021 cagtacgctt gattccatcc ctttatccat gcagcgacag tttcctgatt taacaccgcg
     1081 ccatcttgac catctgaaaa cccttattgc taagggggca aatcagtgtg cgcgggcagg
     1141 ggataaatta ccggatttac tcgatgaata tatcagagca acaactgaat aatatgatgg
     1201 ctgccgtttc ggttgcgctg cagcctctgg tcagggttgt accaatgacg gcagttgaat
     1261 gggctgatca aaattattat ctgcctaaag aatcctcata cggtgagggc gaatggaaaa
     1321 cgctgccatt ccagatcgcc attatgaact gtatgggtaa cgaccaggtt cgcacggtta
     1381 acctgattaa atctgcccgt gttggctata caaagatgtt gctgggggtg gtcgggtatt
     1441 ttattgagca taaatcccga aacagtctgc tttttcagcc cacggattct gccgctgaag
     1501 attttatgaa gtctcacgtg gaggcgacga ttcggaacgt gccatgcctg aaagaccttt
     1561 ccccatggct gggtcgtaaa catcgtgaca atactctcac gctgaaacgc ttttcatcgg
     1621 gcgtcggttt ctggtgcctg ggcggcgctg ccgccaaaaa ctaccgtgaa aaatccgtgg
     1681 acgtggtctg ctatgacgaa ctttcctcgt tcgagccgga tgtcgaaaaa gagggctcgc
     1741 caaccctgct gggggataag cgtattgagg ggtcggtgtg gccaaaatcc attcgcggct
     1801 cgacgcctaa aatcaaaggc acctgccaga tcgaaaaagc cgctaacgag tcggcgcatt
     1861 ttatgcgttt ttatgtgccc tgcccgcact gtggggaggc gcagtatctg aaatttggcg
     1921 atgagtccac gccttttggg cttaaatggg agaaggacag ccctgaaagt gttttctacc
     1981 tctgtgaaca tcatggctgc gtgatccatc agtctgaact ggaccagagc aacgggcggt
     2041 ggatctgtga aaacacgggc atgtggaccc gtgacggtct gacgtttttc agcgcccggg
     2101 gtgatgaaat tccgccgccg cgctccatca cgttccatat ctggacggcg tacagtccgt
     2161 tcaccacctg ggtacagatt gtctatgact ggctggatgc actgaaagat cccaacggcc
     2221 tgaaaacctt tgtgaacacc acgctgggcg agacctggga agaggccgtg ggcgaaaaac
     2281 tcgatcacca ggtactgatg gataaggttg tgcgttacac ggctgcggtg ccttcccggg
     2341 tggtttatct gacggcgggc attgactcgc agcgaaaccg ttttgagatg tatgtctggg
     2401 gatgggcacc gggagaggaa gcctttctgg tggataaaat catcattatg gggcgtcccg
     2461 atgaggaaga gacgctgtta cgtgtggatg ctgcgatcaa caaaaaatat cgccatgcgg
     2521 atggcaccga aatgaccatt tcccgtgtct gctgggacac cggggggatc gatggtgaaa
     2581 ttgtttatca gagatcaaaa aaacactgtg ttttccgggt gctgccggta aaaggcgcgt
     2641 ctgtctatgg caagccggtg atcaccatgc cgaaaacccg caatcagcgg ggcgtgtatc
     2701 tgtgtgaagt ggggacggac accgcaaaag aaattctcta tgcccgtatg aaagccgatc
     2761 ccacgcctgc ggatgaagcc acgtcgtatg ccatccgttt tcctgatgat ccggagattt
     2821 tttcgcagac agaggcgcag caactggtgg cggaagagct tgtggagaag tgggaaaaag
     2881 gaaagatgcg tctgctgtgg gataacaaaa agcggcgtaa cgaagcgctg gactgcctgg
     2941 tgtatgccta cgcggcatta cgtgtgtccg tgcaacgctg gcagcttgat ctggctgtac
     3001 tggcaaaatc ccgggaagaa gagacgaccc ggccaaccct gaaagaactg gcagcgaagc
     3061 tgtccggagg agtgaatggt tacagtcgct gaactgcagg cgctgcgtca ggcgcgcctt
     3121 gatttattaa ccggtaaacg ggtggtgtct gtccagaaag atggtcgcag aattgaatat
     3181 acggcggctt ctctggatga gcttaaccgg gcgatcaatg atgcggagtc ggtactgggg
     3241 acaacccggc gtcgccgtcg tccgctggga gtgaggttat gaaacgaacg cctgtcctga
     3301 ttgatgtgaa cggcgttccg cttcgtgaga gtctcagcta caacgggggc ggcgcaggat
     3361 ttggcgggca aatggcggag tggttgccac cggcgcagag tgccgatgcg gccctgctgc
     3421 ccgcgttgcg tctggggaat gcccgggcag atgatctggt gcgcaataac ggaatagcgg
     3481 ccaatgcggt ggcactgcat aaggatcaca ttgtcgggca tatgtttctt atcagctacc
     3541 gtccgaactg gcgctggctg gggatgcggg agaccgcagc aaaaagcttt gtcgatgagg
     3601 tggaggcggc ctggtcggaa tacgccgaag ggatgtctgg cgagatcgac gtggaaggaa
     3661 aacgcacgtt cacggaattt atccgtgaag gtgtgggcgt tcatgcgttt aacggcgaaa
     3721 tctttgtgca gccggtctgg gatacggaaa ccacgcagtt attccgtacg cgttttaaag
     3781 ccgtgagtcc gaaacgggtg gacacgccag gacacggtat ggggaaccgt tttctgcggg
     3841 ccggggtgga ggtcgatcga tatggccgtg ccgttgcgta ccatatctgt gaggatgatt
     3901 ttccgttctc cgggagtgga cgatgggaac ggatcccgcg tgaacttccc accgggcgtc
     3961 cggccatgct gcatattttc gagccggtgg aggacgggca gacccgtggg gctaatcagt
     4021 tttacagcgt catggaacgg ctgaagatgc tggattccct gcaggcaaca cagcttcagt
     4081 cggccattgt gaaagccatg tatgcagcga cgattgaaag tgaacttgat accgaaaagg
     4141 cctttgaata tatcgccggc gcgccacagg agcagaagga taatccgctt attaatattc
     4201 tggagaagtt ctccagctgg tatgacacga atagcgtgac gctgggcgga gtcaaaattc
     4261 cgcacctttt ccctggtgat gatctgaaac tacagactgc gcaggattca gacaatggat
     4321 tttcggcgct tgaacaggcg ctgctgcggt atatcgccgc cggtcttggc gtttcctacg
     4381 aacagttgtc ccgtgattac tcgaaggtca gttattcaag tgccagggcc tctgccaatg
     4441 agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg gccacgcaga
     4501 tgttttcctg ctggctggaa gaggcacttc ttcgggggat tatccgtccg ccacgggcgc
     4561 gttttgattt ttatcaggcg cgatcagcct ggtcacgggc agagtggatt ggtgccggaa
     4621 gaatggccat tgacgggctc aaggaggttc aggaatcggt gatgcgcatt gaggccggac
     4681 tgagcacgta tgagaaagag ctggcgctga tgggcgagga ttatcaggac attttccgcc
     4741 agcaggtcag ggaatctgca gagcggcaaa aagccggact ctcacgtccg gtgtggatag
     4801 cgcaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag acaacacccc
     4861 gtgagacgta atctttcaca cattattgcc gcggcattca atgaaccgct gcttctggag
     4921 cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgcg agatgggggc agcaagtctt
     4981 tcggtaccac agcagcaggt acagcttgat gctcccggaa tgctggctga aacggacgag
     5041 tacatggccg gaggtaaacg accggcccgt gtttaccggg tggtgaacgg tattgctgta
     5101 ctgccggtga ccggcacgct ggtgcaccgg ctggggggta tgcggccatt ttccggaatg
     5161 acaggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggatag ccaggtgcgg
     5221 ggcgtactgc tggacattga cagtccgggc gggcaggccg ccggcgcgtt tgactgcgct
     5281 gacatgattt accgcctccg tcagcagaag ccggtctggg cactgtgcaa tgacacggcc
     5341 tgttctgcag ccatgctgct ggcgtcggcc tgctcccgac ggctggttac ccagacatcc
     5401 cgtatcggct ccattggcgt gatgatgagc catgtcagct atgccggtca tctggcgcag
     5461 gccggtgtgg atatcacgct gatttactca ggggcgcaca aggtggatgg caatcagttt
     5521 gaagccttac cggcagaggt tcgccaggac atgcagcagc gcattgatgc ggcgcgccgg
     5581 atgtttgccg aaaaagtggc catgtttacc ggtctgtctg ttgatgccgt cacgggaaca
     5641 gaggccgccg tttttgaagg tcagtccggc attgatgccg ggctggcgga tgaattagtc
     5701 aatgcgtcgg atgccatcag tgtgatggcc acggcgctga acagtaatgt cagaggaggc
     5761 actatgccgc aattaactgc aacggaagcc gccgcgcagg agaaccagcg agtgatgggg
     5821 atcctgacat gccaggaagc gaaaggacgt gaacagcttg ccacgatgct ggcaggacaa
     5881 cagggcatga gcgttgaaca ggcccgggcg attctggccg cggcggcacc gcagcagccg
     5941 gtggcatcca cgcagagtga agccgatcgc attatggcgt gtgaagaagc gaacggtcgt
     6001 gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa agcccgcccg
     6061 atcctggctg cttcaccgca ggcggatgcc ggaccctcac tccgtgatca gatcatggca
     6121 ctggatgagg caaaaggggc tgaggcgcag gctgaacagc tggctgcctg cccgggaatg
     6181 actgtggaga gcgcccgggc tgtgctggct gcgggatcag gtaaggcaga accggtctct
     6241 gcatccacaa ccgctctgtt tgaacgcatc atggcgaacc attcaccggc agcggtacag
     6301 ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct catggccatg
     6361 ccatgaagtc agtgctgacc atcaacagga ggtttttaca atatggtaac gaaaaccatc
     6421 actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggctca taccgccaca
     6481 ggcagcagcg ggatttcctc ggcaacaccg gcactgacgc ccctgatgct ggatgaagct
     6541 accgggaaac tggtggtctg ggacggacag aaagccggta gtgcggttgg catactggta
     6601 ctgccgcttg aaggcacaga gacggtactg acgtattaca agtcggggac ctttgcgacg
     6661 gaggcaatcc gctggcctga cagtgtggat gaacacaaaa aggcaaatgc ctttgccggc
     6721 agtgccctga gtcacgcggc gctgccgtaa cacgttatca ggccaccgcg gtggcctgac
     6781 tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca gttactcggt
     6841 tataccgaac aaaaagtgaa atttcgtgcg ctgtttctgg agctgttttt ccgccgtacg
     6901 gtgaatttcc ataccgaaga ggtgatgctg gacaaaatta ccggaaaaac gccggtggcg
     6961 gcctatgtct ccccggttgt tgaaggaaaa gtgctgcgtc atcgcggtgg tgaaacccgc
     7021 gtgttacgtc cgggctacgt caagccgaaa cacgaattta attaccagca ggcggttgag
     7081 cgtcttcccg gtgaagatcc ggctcaactg aacgacccgg cctaccgtcg tctgcgtatc
     7141 atcaccgata acctcaaaca ggaagagcat gccattgtcc aggtggaaga aatgcaggcg
     7201 gtgaatgccg tgctgtatgg caaatacacc atggaagggg atcagtttga tactgtcgag
     7261 gtggattttg gacgctctga aggaaataac attgagcagg ccgacggtaa aaaatggtct
     7321 gagcaggacc gtgatacgtt tgatccgacg catgatattg acctctactg cgatcaggcc
     7381 agcggtcttg tgaatattgc cattatggac ggtactgtct ggcgtctgct gaatggtttt
     7441 aagctgttcc gcgaaaaact ggatacccgt cgcggctcaa attcacaact cgaaacggca
     7501 gtgaaagacc tgggggcggt ggtgtctttc aaagggtatt acggcgatct ggccattgtg
     7561 gtggcgaaaa cgtcttatgt ggcagaggac ggtaccgaaa aacgttatct gccggagggc
     7621 acgctggtcc tgggaaatac ggctgcagat gggatccgtt gttacggtgc cattcaggat
     7681 gcgcaggcgt tgtccgaagg tgtggtggct tcttcccgtt acccgaaaca ctggctgacc
     7741 gtgggcgatc cggcccgtga atttaccatg acgcagtccg caccgctgat ggtgctgccg
     7801 gacccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcggggg cgaaatgccc
     7861 ccgtgtcttt tttcacaggg ggctgatatg gcaacaaaag aagaaaatca gaaacgtctt
     7921 cgtcaactgg ctggcctgct ggggcgcgag gcggatatgt cggggagtgc tgcggatatt
     7981 gcgcaacgtg tgtctgagtg ggaagaggag cttgctgctt cccgggaggg cattatgcct
     8041 ggtgatgaga gcgggcctga gcaaaatcac acagacgatg gtgagcagtt gcacaacact
     8101 gatgctacgg atgatgttaa agcggtccgt gtgcggaaat gcctgcatgt gatggggtat
     8161 tgcccggaga caggccgtcc cgttgaactg acgtaccggg gcatgcgtgt tctggtgcca
     8221 tcaccactgg cgacagccat gatacagcac ggaacggctg agcatgcgtg attttcagaa
     8281 tgcctttgat gctgcccttg ccggggtgga cagcacgatt gttgaagtga tggggctctg
     8341 tgcgcagttc acctcggggg cacagtgtgg cagcgaagtt cagggggttt ttgacgatcc
     8401 ggagtcgctg gggtttgccg gtagcggggt ccgtattgaa ggaagctgcc cgtcattatt
     8461 tgtacggacg gatacggttc gtgctgtgcg gcgtggtgac acgctgacca ttaatggtga
     8521 gacattctgg gtggatcgtg tttctccgga tgacgggggc agttgttatc tctggctcaa
     8581 ccgtgggcaa ccaccggcag ttaaccggcg acgataaacg cagggtgaaa ttatggcgat
     8641 aaaagggctt gatcaggcga ttgacaatct gagccgggtt cgtaaaaacg ccattccggc
     8701 ggcttcagca atggccatta accgcgtggc cacaacggcg attaatcagt cttcatcaca
     8761 ggttgcccgg gagacaaagg ttcgccggaa actggttaag gaacggtcca gactgaaacg
     8821 ggcgacggtc agaaatccga atgccagaat tatcgttaac cgcggtgatc tccctgtgat
     8881 taagctgggg atcaggatgc cggggcgtcg cccgaacagc atacttaaag ccggtcagca
     8941 tcggtatcag cgggcattta ttcagcgatt aaaaaatggt cgctggcatg tcatgcagcg
     9001 tgtggccggg aaaaaccgtt accccattga tgtggtgaaa atcccgatgg cggccccact
     9061 gaaacaggca tttgatgaga atgttgaccg tatccggcgt gaacgactgc ctaaagaact
     9121 ggcatccgcg ctgaaacaac aactgaggat tgcaataaaa cgatgaaaca cactgacatt
     9181 cgtgccgcag tgctggatgc actcgagcag catgaacacg gggcgacgct gtttgatggt
     9241 cgccccgttg tttttgacga agaggatttt cctgcgatcg cggtttatct gacggatgca
     9301 gagtataccg gtgaagagct ggatgcagat acctggcggg ccacgctgca tattgaggtg
     9361 tttttaccgg cacaggtacc ggattcagag cttgatcagt ggatggaaag ccggatttac
     9421 ccggcgatga ccgc
//
LOCUS       DAZKIV010000164         9332 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZKIV010000164 DAZKIV010000000
VERSION     DAZKIV010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.164"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(352..1545)
                     /gene="tyrB"
                     /locus_tag="ACHOT6_004011"
     CDS             complement(352..1545)
                     /gene="tyrB"
                     /locus_tag="ACHOT6_004011"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFP9949153.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLKQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1798..2877)
                     /gene="alr"
                     /locus_tag="ACHOT6_004012"
     CDS             complement(1798..2877)
                     /gene="alr"
                     /locus_tag="ACHOT6_004012"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFP9949154.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2930..4345)
                     /gene="dnaB"
                     /locus_tag="ACHOT6_004013"
     CDS             complement(2930..4345)
                     /gene="dnaB"
                     /locus_tag="ACHOT6_004013"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFP9949155.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4428..5411
                     /gene="qorA"
                     /locus_tag="ACHOT6_004014"
     CDS             4428..5411
                     /gene="qorA"
                     /locus_tag="ACHOT6_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFP9949156.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5577..5819)
                     /gene="pspG"
                     /locus_tag="ACHOT6_004015"
     CDS             complement(5577..5819)
                     /gene="pspG"
                     /locus_tag="ACHOT6_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFP9949157.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(5953..6990)
                     /gene="dusA"
                     /locus_tag="ACHOT6_004016"
     CDS             complement(5953..6990)
                     /gene="dusA"
                     /locus_tag="ACHOT6_004016"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFP9949158.1"
                     /translation="MHDNPETQKTNQTSVMPEKTGAYWSSRFSIAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            7079..8176
                     /locus_tag="ACHOT6_004017"
     CDS             7079..8176
                     /locus_tag="ACHOT6_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9949159.1"
                     /translation="MAYYNIEKRLKSDGTPRYRCNVIIKEKGVITYRESKTFPKHAHA
                     KTWGAQKVMELDLYGIPSSNAVDGLTVRDLLHKYLNDPNAGGKAGRTKRYVLELLMDS
                     DISAIKLSELTENDVIEHCRLRNNAGAGPATVSHDVSYLGSVLDAAKPVYGINYTSNP
                     AKSARPYLLKLGLIGKSNRRNRRPASDELDMLIEGLQQRSTHKCSKIPFVDILKFSVW
                     SCMRIGEVCRLRWEDLDQEQKSILVRDRKDPRKKEGNHMKVALLGEAWDIVQRQPKKS
                     EFIFPYNSTSVTAGFQRVRSKLGIKDLRYHDLRREGASRLFEAGFSIEEVAQVTGHRS
                     LNVLWQVYTELYPKSLHNRFEELQKSRNKTS"
     gene            8238..8486
                     /locus_tag="ACHOT6_004018"
     CDS             8238..8486
                     /locus_tag="ACHOT6_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9949160.1"
                     /translation="MRIEICIAKEKMTKMPNGAVDALKEELTRRISKRYDDVEVIVKT
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            8623..8706
                     /locus_tag="ACHOT6_004019"
                     /pseudo
     CDS             8623..8706
                     /locus_tag="ACHOT6_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(8732..9322)
                     /locus_tag="ACHOT6_004020"
     CDS             complement(8732..9322)
                     /locus_tag="ACHOT6_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFP9949161.1"
                     /translation="MPMNTTGTSFSSFGISCHRENSFRNSFLGKNDEVIKCSMGERTI
                     HFSVRKFSGNILDTVNRQNTKDINGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSHRYQLTLDVKAAQSSINHVIMGNASFGKKIDTLCDSMSRDVKNRTADCI
                     ANSLADKFYQKHIEPDIDIVKLRNEIPDYLRCTIQA"
BASE COUNT         2492 a         2340 c         2203 g         2297 t
ORIGIN      
        1 gagctctctt taactttaga atttaacgat actataattg aatctcagaa agtcacaggc
       61 aataaggcaa agcatattct tgaggatata gaaaaattct atcgtaacaa acccgatact
      121 tattattaaa tttaataata gttagatgtg cagaaattat tactccaccc aaaaaaacag
      181 gataaggaat tccagataat taggataagc ttaacaataa aagaactaat aacgtttatt
      241 atctctcacg tagaacgatg gcatcaaaaa aaatcatcta taaagaaaga gtgaaagcga
      301 cgacagttta atttcactgc aggctgggta gctccagcct gctttcctgc attacatcac
      361 cgcagcaaac gcctttgcca cacgttgtac atttgccgta tttaacccgg cgacacacat
      421 gcgaccgctg gcgatgagat agacaccaaa ttcttcacgt agtcggtcaa cctgagcggc
      481 acttaaaccg gtataactga acatgccgcg ctgattaagc agataatcga aattgcgttc
      541 tggcatctct gtgcttaata ccttcaccag ttcctgacgc attgccagaa tgcgagtacg
      601 catctcttct acttccgcca gccagctggc tttcaatgcc tcgtcattca gcactgcagc
      661 caccacctgc gcaccaaaat tcggcgggct ggagtagttg cggcgaactg ttgctttcaa
      721 ttgccccagt acgcggccag cggcttcggc atcttcacac ataacagaaa gtccgccgac
      781 gcgctcgccg taaagggaga aaattttcga gaacgaattg ctcaccagag cgggtaatcc
      841 agcgctggca atggcgcgaa tagcgtaggc atcctcttcc ataccggcac caaatccttg
      901 ataggcaata tcgaggaatg gaataagctc gcgggctttg agaatttcaa tcaccgcatc
      961 ccactggtca ttagtgagat cggcacccgt tgggttgtgg caacatggat gcagcaacac
     1021 aatactgcgg gcaggtaatg ttttcagcgt cgccaacagg tcattaaagc gcacgccgtt
     1081 agtcgcttcg tcataccagg ggtaagtact cacttcgaat ccagccccgg cgaatattgc
     1141 tacgtggttt tcccaggtag gatcgctgac ccagacgcct gattccggga agtagcgttt
     1201 caggaaatcc gcgcccactt tcaatgcccc ggagccgcca agggtttgaa tggttgctac
     1261 gcgctgttgt ttcagtaccg gatggtccgc accaaacagc agcggcgcaa tggcatggcg
     1321 atagctgtta agcccttcca tcggtaaata aagcgaagcg ccatgaggct gcgcattcag
     1381 gcgcgcttcc gcctccgcca cggctttcag ttgtggaata attccgtctt cgttgtagta
     1441 cagaccgata cttaaattca ctttgtcgct gcgagggtct tctttaaaac gctccataag
     1501 cgtaagaatc gggtcgccag cgtaggcgtc aactttttga aacacgcgat ggttctccag
     1561 gtttacgggc aggtggttaa gacacaataa accggaagaa gacgaagatc gagtggatgt
     1621 tcaggagcga acggtaatta gcaacagagt gagactcatg acaaacgtac attcgccaga
     1681 ggctcgacct ttataagagc atgaaagaat atcaacttat tgaattggta ggattttatt
     1741 ggccggacaa ggcattcact ccgcatccgg cacagacaaa caaatattac agaacgatta
     1801 atccacgtat ttcatcgcga cccttgaagt caggcgagtg ataagttcgt aagcgctcac
     1861 ttttgtcatt tcagcgatac gttctacggg caaaccttcg ccccataaaa tgaccggatc
     1921 cccggctttg tcctgcgctt gtggacctaa gtctacgcag atcatatcca tcgccacgcg
     1981 cccgacaatc ggtacttcgc gaccgttcac cagcactggc gtaccggacg gcgcggcgcg
     2041 cggataacca tcgccatagc ccatcgcgac tacgccaaga cgggtatcac gttcgcttac
     2101 ccaggttcca ccataaccaa caggttctcc ggctttatgc tcacgcacgg caatcaggct
     2161 ggaggttagt gacatcactg gctgacagcc aaaatcggca ccggtggagc gatcttccag
     2221 cggcgagacg ccataaagaa tgatgcccgg gcgcacccag tcaaaatgcg actgtggcca
     2281 cagcagaatg ccacccgatg cggcaatgga acgttgacca ggtttgcctt cgcaaaaggt
     2341 attaaagata gcgagttgtt tctcggttgc gccacatttt ggttcatccg cgcgcgcaaa
     2401 atggctgacg atattcaccg gctgacgaac gtttttgcac tgggtcaggc gatgataaaa
     2461 cgcctcagcc tgttccggcc ttacgcccag acggtgcata ccggtatcga gtttcatcca
     2521 gacggtaacc ggctcgtcca ggctagcctc ttccagcgca gccagctgtt cttcgttatg
     2581 cacggcggta tgaaaatgtt gcgcagaaat cgtcggcaga tctctggcat caaaaaagcc
     2641 ttcgagtaac agtacaggtt tggtgattcc ccccgcacgc agtcgcagag cttcttcgag
     2701 acgggctacg ccaaaggcgt cagcatcggg gagcgttcgc gcggtctcaa gaagaccgtg
     2761 accataagcg ttcgctttca ccaccgcaac cattttactg gcaggggcca gttcacgaag
     2821 acgttgcagg ttgtgtcgca gagcgcggcg gttaatcaca acagttgccg cttgcatttg
     2881 tgttccttga taagtgtttg ctttaattac ctaattcata aaataattat tattcgtcgt
     2941 cgtactgcgg ccccgcatag ttgtcgaagc gcgaccattg accgttaaag gtcaggcgta
     3001 ccgtcccgat tgggccgtta cgttgtttac cgataataat ttccgcgatg ccttttaaat
     3061 cactgttttc gtgatacacc tcatcacgat agataaacat gatcaagtcc gcatcctgct
     3121 cgatagagcc agattcacgc aggtcggagt tgaccgggcg tttgtcggca cgttgttcca
     3181 gagaacggtt caactgggac agcgccacca ccggcacgtt cagttctttc gccagtgctt
     3241 tcagcgagcg agagatttct gcaatttcca gcgtacggtt atcggaaagc gccggtacgc
     3301 gcatcagttg caggtagtcg atcatgataa gcccgatgcc gccgtgttca cgggcaatac
     3361 ggcgtgcgcg ggaacgcact tccgttggcg tcaggccgga ggagtcatcg atatagatat
     3421 tgcgtttttc gagcaaaata cccatggtgc cggaaatgcg cgcccagtct tcatcatcga
     3481 gctgcccggt acggatttta gtctggtcaa cgcgcgacag cgacgccaga gaacgcatca
     3541 taatctgttc tgaaggcatc tccagcgaga agataagtac tggtttatcc tgcaacatcg
     3601 ccgcgttttc gacgaggttc atcgcaaatg ttgttttacc catcgacgga cgcgcggcga
     3661 cgatgatcaa atccgacggc tgcaagccag cggttttttt gttgagatcg tcataaccgg
     3721 tgtttactcc ggtaacgcca tcgtgtggct gctgaaacaa ctgctcaata cgcgccacgg
     3781 ttgcgtcgag cacatcggcg atgttcttcg gcccttcgtc tttgtttgca cgactttcgg
     3841 caattttaaa gacgcgggat tcagcaaggt ccagcagatc ttcgctggta cgcccctgcg
     3901 gatcaaaacc ggcttcagca atctcattcg caaccgagat catctcacgg acaacggcac
     3961 gttcacgcac gatgtcagca taagcactga tgttcgccgc acttggcgta ttttttgaca
     4021 gctctgccag ataagcaaaa ccaccgacgc tatcgagttg cccctggcgt tccagcgatt
     4081 ccgcaagggt aatcagatcg ataggactac cgctttcctg caaacgcgcc atttcagtaa
     4141 agatatggcg gtgtgggcgg gtgtaaaaat cgtctgccac cacacgctcg gctacatcgt
     4201 cccagcgttc gttatccagc attaaaccgc ccaacaccga ctgctccgct tcgatcgagt
     4261 gcggaggcac tttcagcccg gcaacttgtg gatcgcgttc gcggggttca gcctgctgtt
     4321 tgttgaaggg tttatttcct gccatagtga atggagttac cgagataaag aatgggtcga
     4381 aactttacca tatgaagcag accctgacga tacgttctgg aggacacatg gcaacacgaa
     4441 ttgaatttca caagcacggt ggcccggaag tacttcaagc cgtagagttc actcctgccg
     4501 atccggcaga gaatgaaatc caggtcgaaa ataaagccat cggcatcaat tttatcgaca
     4561 cgtatatccg cagcggcctt tacccgccgc catcactacc cagcggatta ggtaccgaag
     4621 cggcaggcat cgtgagtaaa gtcggcagtg gtgtaaagca tattaaggca ggcgatcgtg
     4681 tagtctatgc gcagtcggcg ttaggcgctt acagctctgt gcataacatt aatgcggata
     4741 aagcggcgat tctgcctgcg gcaatttctt ttgagcaagc tgcggcatcc ttcctgaaag
     4801 gcttaacggt ttattatctg ctgcgcaaaa cctatgaaat taaacccgat gaacagttcc
     4861 tgttccacgc agcggctggc ggcgttggct taattgcctg ccagtgggca aaagccctgg
     4921 gcgcgaaact tatcggcacc gtaggaaccg cgcaaaaagc gcagagcgcg ctaaaagcgg
     4981 gcgcgtggca ggttattaac tatcgtgaag aggatctggt cgagcggtta aaagagatca
     5041 ccggcggtaa gaaagtgcgc gtggtgtacg attccgtggg cagagacacc tgggaacggt
     5101 cgctggattg cctgcaacgc cgcggcttaa tggtcagttt tggcaactca tcaggtgcgg
     5161 ttaccggtgt gaacttaggc attctcaatc aaaaaggctc gttgtatgtg acacgccctt
     5221 ccctgcaagg ctatatcacc acgcgggagg aattaaccga ggccagtaat gaactgttct
     5281 ctttgattgc cagcggtgtg attaaggtcg atgtcgccga gcagcagaaa tatccgctga
     5341 aggatgcgca gcgtgcgcat gagattctgg aaagccgggc gacgcaaggt tccagcctgt
     5401 tgattccata aaagaaatag ggcttccacc tgggaagccc tttcttttta tagttcggct
     5461 gtatgtaggg tacagcacga tgaatctgtt agaggcgcaa tagtgacaga tttgattatc
     5521 aattcctatt ttgttctaag gataaaacct taggttgtga tcatccgcac aatcccttag
     5581 taacgccagc ggtcataacg ctgatatttc ggcatttttg gtgctttaat cgccttaata
     5641 acccacacca ccgcaatcgc cagtagtaac cacggcagca acttaatcat caatgccagc
     5701 ataccgccga ggaacataat ggccgtcgcc acaaccagcg cggcgataat gcccagcaac
     5761 gaaacgccgg tgaccatcag catgacaaaa aagccaatca caaaaagtag ttccagcatg
     5821 atgctctccc aaatatgaaa tctcttgctg gcattacaag aatcatgcca aaaataatct
     5881 attgatttaa cagcaaaacg ccccgcgacg gtgcgcaggg cgtggtgaat ttgactactt
     5941 tttggtgaaa agttaacgct tatccgccac cagtttgagc gcgtgttcca gcacgttaat
     6001 gtcagcaccc gctttatggg cattttcact taaataacgc cgccactgcc gcgcgccagg
     6061 aataccctgg aacaaaccca gcatatgccg ggtaatatgg ccgagatacg tcccctggct
     6121 gagttcacgc tcaatgtacg gatacatggc gcgcactacc gccaccggat cggcatcggt
     6181 atccgaggaa ccaaagatct cccggtctac cgccgccaga atacccggat tctgatacgc
     6241 ctcgcgcccg accatcacgc catccatatg ttgcaggtgt gctttggcct cttccagcga
     6301 cttgatacca ccgttaatcg acattgtcag atgcggaaag tcacgcttca gttgatacac
     6361 acgcggataa tcgagcggcg ggatttcacg gttttctttc gggcttaacc ccgaaagcca
     6421 ggctttacgt gcgtggatga tgaacatctc acactcgcct ttgccggaaa cggtattgat
     6481 gaaatcgcag agaaattcat agctgtcctg gtcgtcgatg ccaatacgcg ttttcaccgt
     6541 caccggaatc gacaccacat cgcgcatcgc tttcacgcag tcggcaacca gctgcgcatt
     6601 acccatcaga cacgcaccaa acatgccgtt ctgcacccgg tcagacgggc agccgacatt
     6661 caggttgatc tcatcatatc cgcgcgcttc tgccagcttt gcacactgcg ccagcgccgc
     6721 cggatcgcta ccgcccagtt gcaacgctac cggatgttct tcttcactgt acgccagata
     6781 atcaccttta ccgtgaataa tcgcccctgt ggtcaccatt tcggtataaa gcaacgtatt
     6841 gcgggaaagc agacgcaaga aatagcggca atgtctgtcc gtccagtcga gcataggagc
     6901 aatgctaaac cgagaactcc agtaagcgcc agttttttca ggcatcacgc tggtttgatt
     6961 agttttctgt gtttcaggat tatcgtgcat ttttgaacat ttcaggctat ttttctcgcg
     7021 ttaggttccc gcacaggttc ccacgtttta tgggaacccg aaataacgag gtcgtgtaat
     7081 ggcgtactat aacatagaga aacgactaaa atccgatggc acaccacgct atcgctgtaa
     7141 tgtgattatc aaagaaaaag gtgttatcac ttacagagaa agcaaaacat tccctaaaca
     7201 tgctcatgcc aaaacatggg gcgcacagaa agtgatggaa ttagatctat atggcattcc
     7261 atcatcaaat gctgttgatg gacttacagt ccgtgactta ctacacaaat atttaaatga
     7321 cccaaatgcc ggaggtaaag caggccgaac taaaagatat gtgctggaac tgcttatgga
     7381 tagtgacata tccgcgatca aactatctga actgacagaa aatgacgtaa ttgaacattg
     7441 caggctaaga aacaacgctg gtgcaggccc agcaacagtc agccacgatg ttagttatct
     7501 tggcagtgtt ctggatgcgg caaaacctgt atacggaatc aattacacat caaacccggc
     7561 gaaaagcgct cgtccatatc tacttaaact cggtttgatt ggtaaatcaa accgtcgtaa
     7621 tcgtagacca gcatctgatg aactggacat gctcattgaa ggccttcaac aacgatctac
     7681 tcataaatgc tcaaaaattc cgttcgttga tatcctcaaa ttttctgtgt ggtcctgtat
     7741 gcgaatcgga gaagtatgcc ggttacgatg ggaagatctc gaccaagaac aaaaatctat
     7801 actagtaaga gataggaaag atccacgtaa aaaggaaggt aaccatatga aagttgcctt
     7861 gcttggggaa gcctgggata tcgtccagcg acaaccaaaa aaatcagaat tcatttttcc
     7921 atataacagc acttctgtta ccgcaggatt tcagagggta agaagcaaat taggtattaa
     7981 agatctgcga tatcatgatt tgcgtagaga aggggcaagt cgcttatttg aggctggttt
     8041 tagtattgag gaagtcgctc aagttacagg gcatcgttca ttaaacgtgc tatggcaggt
     8101 atataccgaa ctgtatccga aatctttaca taatcgtttt gaagaactcc aaaagagcag
     8161 aaacaagacc tcttgacact gtttatttat acagacaaaa ataacactgt atacaaacac
     8221 agtgtagagg gatttttatg cgtattgaaa tctgcatagc caaagagaaa atgactaaaa
     8281 tgccaaacgg tgctgtggat gcgttaaagg aagaattaac tcggcgcatc agtaagcgtt
     8341 atgacgatgt agaggtgatc gtaaaaacca ccagcaacga tggcctttct gttacgcgca
     8401 ctgccgataa agattcagct aaaacttttg tccaggaaac tctgaaagat acctgggagt
     8461 ctgctgacga gtggtttgtt cgctaattaa cgagtaaaat cggtaacggc tggaaatcat
     8521 tcaatactcg cactatcgaa aaattcacca gccaacagcg gtaagttctt tcatacgacg
     8581 taccgctgtt ttctttatga tttatagctg tactggtaaa ttatgagcaa tctgaatcca
     8641 tgcatgacgt atggcgcctg ttgtgcattt ttccgcgtct ctttttacca gagcaaaacc
     8701 aatgatactg acgtagtcgt ctcccagaga cttatgcctg tatagtacac ctcaaataat
     8761 ctgggatctc atttcgtagt tttacaatat caatatccgg ctcgatatgt ttctggtaaa
     8821 acttgtctgc aagcgagttt gctatgcaat ctgccgtacg attttttaca tcccggctca
     8881 tactatcaca aagagtatct attttcttgc caaaagaagc attccccatt attacatgat
     8941 taatagaact ttgtgctgcc ttaacatcaa gagttagttg atatctatgg ctcacaagag
     9001 aaaagaccct ttgcctctct tcagtggaaa tttttgcatt tttttgaaaa cagtaattat
     9061 caatctcctg gttgatcacc cttgagggat atactatccg ttcatctttt atccacccat
     9121 taatatcttt agtattctgc ctgttcactg tatccagtat attgccgcta aatttgcgaa
     9181 cagaaaagtg aatcgtccgt tctcccattg aacattttat gacctcatca ttcttcccta
     9241 ggaaagagtt cctgaaggag ttttctctat ggcaacttat accaaaagag ctgaaagacg
     9301 tacctgtagt attcatcggc attataattc ct
//
LOCUS       DAZKIV010000165         9176 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZKIV010000165 DAZKIV010000000
VERSION     DAZKIV010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.165"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            192..344
                     /gene="hokA"
                     /locus_tag="ACHOT6_004021"
     CDS             192..344
                     /gene="hokA"
                     /locus_tag="ACHOT6_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFP9949162.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(333..392)
                     /locus_tag="ACHOT6_004022"
     CDS             complement(333..392)
                     /locus_tag="ACHOT6_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949163.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(532..744)
                     /gene="cspA"
                     /locus_tag="ACHOT6_004023"
     CDS             complement(532..744)
                     /gene="cspA"
                     /locus_tag="ACHOT6_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFP9949164.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1025..1315)
                     /gene="yiaG"
                     /locus_tag="ACHOT6_004024"
     CDS             complement(1025..1315)
                     /gene="yiaG"
                     /locus_tag="ACHOT6_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFP9949165.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1749..2459
                     /gene="yiaF"
                     /locus_tag="ACHOT6_004025"
     CDS             1749..2459
                     /gene="yiaF"
                     /locus_tag="ACHOT6_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFP9949166.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2509..3483)
                     /gene="ghrB"
                     /locus_tag="ACHOT6_004026"
     CDS             complement(2509..3483)
                     /gene="ghrB"
                     /locus_tag="ACHOT6_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFP9949167.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNAHRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3587..4246)
                     /gene="yiaD"
                     /locus_tag="ACHOT6_004027"
     CDS             complement(3587..4246)
                     /gene="yiaD"
                     /locus_tag="ACHOT6_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFP9949168.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4399..6732
                     /gene="bisC"
                     /locus_tag="ACHOT6_004028"
     CDS             4399..6732
                     /gene="bisC"
                     /locus_tag="ACHOT6_004028"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFP9949169.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6701..7141)
                     /gene="yiaC"
                     /locus_tag="ACHOT6_004029"
     CDS             complement(6701..7141)
                     /gene="yiaC"
                     /locus_tag="ACHOT6_004029"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFP9949170.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7138..7701)
                     /gene="tag"
                     /locus_tag="ACHOT6_004030"
     CDS             complement(7138..7701)
                     /gene="tag"
                     /locus_tag="ACHOT6_004030"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFP9949171.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7859..8557
                     /gene="yhjY"
                     /locus_tag="ACHOT6_004031"
     CDS             7859..8557
                     /gene="yhjY"
                     /locus_tag="ACHOT6_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFP9949172.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGLS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8786..>9176
                     /locus_tag="ACHOT6_004032"
     CDS             8786..>9176
                     /locus_tag="ACHOT6_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9949173.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFP"
BASE COUNT         2206 a         2251 c         2359 g         2360 t
ORIGIN      
        1 tatcaaggag aagaaacaaa cttattaagc tagaatagcc acgggtgctt gagactgttt
       61 gtctcaggta ttcaccgaaa ggcagacaga gaaaagcccc acctgactat aaatcaaagt
      121 gaggctaccc tatgcctgaa caccataagg ttagcctctt actcgttgga aatcaacaca
      181 gggggctggg aatgccgcag aaatatagat tactttcttt aatagtgatt tgtttcacgc
      241 ttttattttt cacctggatg ataagagatt cactgtgtga attgcatatt aaacagggga
      301 gttatgagct ggcggcattt ttagcctgca atttaaaaga gtaagagtct tcggcgggaa
      361 gttattcccg cctttcttac ggcgttgcgc attctcattg cacccaaatt tatttttcac
      421 aaaaataata ctagatttta ttacgcgatc gattatttat ttcctgaaaa acaaataaaa
      481 aaatccccgc caaatggcag ggatcttaga ttctgtgctt ttaagcagag attacaggct
      541 ggttacgtta ccagctgccg ggcctttagc gccgctttcg atggtgaagg acactttctg
      601 accttcgtcc agagatttgt aaccatcgtt ctggatagca gagaagtgta cgaacacgtc
      661 tttagagcca tcgtcaggag tgatgaagcc gaagcctttg tcagcgttga accattttac
      721 gataccagtc attttaccgg acatagtgta ttacctttaa taattaagtg tgcctttcgg
      781 cgatatggcg tgctttacag attttgaagc gttaaaggaa tgtgcactac gaggggtatc
      841 aacgataact cttgaaggga cttgccttac tacactgctt taatggtctg tacgtcaaac
      901 cgttgatgtg cattaagcca cgcattggcg ggtgatgcaa caattatttt tcatatttat
      961 gattaatcgg ttggaaaacg gtcctgtcat caggaccgta aacagcaata aagtggataa
     1021 aagtctattc catcaactgc ttacttaatg ccgggttggc ttgaatcaaa cgcatcaatt
     1081 ttagttcggc acttgaaggc ttcacgcgtc tggattccca ttcctttacc atggcgactg
     1141 atacgcccaa aacccgggcg aaatcatcga tttttaaccc tgtccctttt cgtaactgct
     1201 caatttcggt acaggacgtt gttctgtgcg tcagggtaat tttctgcgtt tcatctttaa
     1261 aaacaatctg ttccaggctg ctcaacagct catgcattgg atctttatat tccattgaaa
     1321 actcctcaaa tcacactgcg ggatcgtgaa ttgcatcgaa gctcattaag aatagtcggg
     1381 aaaagcagac acagcggggt gaagtcaggg catgctctgg ctatgattcg attcagccag
     1441 tcatttagcg tgacttatag ccacctaacg atctgataaa tgccgatata tctcttacca
     1501 ctcctggttt gtaaagattt cgaaaatcta accttctgcg aaggcgtaaa tctctggcta
     1561 caaaaaaagc aacggcacta tcatttttta acaatgaaac aacaaattaa ccttgtttca
     1621 ttttctccat gattttacca ggaagattac gtagaaaggg tatcctgcaa gcctgtcctg
     1681 gactatcctt gtcacgtcag acacgcgtgt gtcgttgtgc gctttttttg ggtgaaagga
     1741 gtaagaaaat ggcgacagga aagtcctgct ctcgctggtt tgcgcctctt gcggcgttat
     1801 taatggtagt tagcctgagt gggtgttttg ataaagaagg cgatcagcgt aaagcgttta
     1861 tcgacttcct gcagaataca gtgatgcgta gcggtgaacg tctaccaacc ctgactgccg
     1921 atcagaaaaa acagtttggt ccttttgtct ctgattacgc gattctgtat ggttattctc
     1981 agcaggtgaa tcaggcgatg gattccggtc tgcgcccggt cgtagacagc gttaacgcaa
     2041 ttcgcgtacc gcaggattac gttacgcaaa gcggtccgct gcgtgaaatg aacggttcgc
     2101 tgggtgtgct ggcgcaacaa ctgcaaaatg cgaagttgca ggccgatgct gcgcactctg
     2161 cgctaaaaca gaccgatgat ttgaaaccgg tctttgatca ggcattcacc aaagtggtaa
     2221 caacgccagc tgatgcattg caaccgttga ttccggctgc acaaactttc acgcaacaac
     2281 tggtgatggt tggagactac attgctcaac aaggtactca ggtgagcttt gtagcaaatg
     2341 gtatccagtt cccgacttcg cagcaggcaa gtgaatataa caaactgatt gcgccgttac
     2401 cggcacaaca tcaggcgttt aatcaggcct ggactacagc agttactgca acccaataaa
     2461 gagtaaaagc ccgagcgatt atcgggcttt acttcacgca gtcgcggctt agtccgcgac
     2521 gtgcggattc acacagttct tctcaacctt tccttgtaac gcatcaatca aattatccac
     2581 ggcacaggcg gccatgccat aacgcgtctc atgggtggca gatccaatat gcggtactgc
     2641 gacgacgttg gccattgaga gcaacggcga atctacggac agtggctctt gttcgaagac
     2701 atccagcccg gcggcgtgaa tttcgccttt ctgcaatgca gcaatcagtg cattttcatc
     2761 aaccaccggg ccacgtccgg cattaatgaa aatggcggag gatttcattt tggcgaattg
     2821 ttctgcgcca aacagatgat gcgtctcatc agttaacggc aggatcaggc aaacgaaatc
     2881 tgactcttgt aacagagtat ccaaatcgca gtagcgggcg ttgaagcgtt cttctgcttc
     2941 tttatggtgg cggtgcgcgt tatagaggat gggcatgttg aagccaaagt gcgcacgttg
     3001 tgccagcgcc ataccgatcc gtcccatccc gacaatgccc agtgttttat ggtgaacgtc
     3061 agtgccgtac cagtccgggc ctatgctcgc ggtccattcg cctgctttta cccgttctgc
     3121 tacctccaca acccgacgag cggtagacaa caccagcgcc atcagcgtat cggcgacggt
     3181 ttctgttaat acggttggcg tgtgcatcag cagaattttt cgggcggtaa gcgcatcgac
     3241 atcaaaattg tcatagccga cggagatcgt tgatgtggca cgcagtttcg gcattttttc
     3301 cagcaatgcg gcatctacat tctcgtttga acccagtaaa ccttcagctt cggcaaaaat
     3361 tgctgcattt tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa cggtgaaatg
     3421 ctcttgcagg cgttgcagta aatcatcagg taaggctttg tagaggataa cggacggctt
     3481 catgcttctc tccattcact gataaggcga tcaccttagc ctgaaatatg atgaaggaaa
     3541 actgaagaat gccaggtgat ttctgcatca cctggcatga aagggattac agcgggctta
     3601 aggtaatttc tacacggcgg ttttgcgcct taccttctgc ggtgctgttg ctggcgattg
     3661 ggttagccgg gccaaggccc tgagtacgga tgcggctggc gtccacgccc tgggtgatca
     3721 acgcgctggc aacggaatcc gcacgttgct gggagagacg catgttcagg tcgtgacccc
     3781 ccgtgctgtc ggtataacca atcacgttaa ccgccgtttt cggatactct ttcagcacca
     3841 ttgccacgcc ggtcagggtg ttagcgcctg ccggtttcag ggtcgcgctg ctgctgtcga
     3901 aggtcacatt gttcggcata ttgaggataa tgttatcccc gctgcgggtt acgctaacac
     3961 cagtgccgcg cattttgtca cgcagcttcg cttcctgcac atccatgtaa taacccacgc
     4021 cgccgcccag agctgcgcct gctgctgcgc caatcagcgc gcctttaccg cgatctttct
     4081 tcgaagaaga gagcgcacca atacccgcgc ccacgagaga gcccagacct gcgccgatag
     4141 cagatttgcc tgcttcgcgt tcgccggtgt aagggttagt tgtgcagcca gataccgcca
     4201 gagcgccact cactacggcg gcaataagat aaacacgttt cttcattgtt aatccttaat
     4261 aaccttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt gaacatgaag
     4321 attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa tacatctaac
     4381 cttacaggag tcttcggttt ggccaactca tcctcacgat attccgttct gactgccgcc
     4441 cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc gcgtggcgcg
     4501 ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt tcacagcaat
     4561 acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga aaacccgcaa
     4621 ggcattcgtg ggcaggatga atttgttcgc gtgagttggg atgaggcgct ggatcttatt
     4681 caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt tgctggttcc
     4741 tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca acgctatatg
     4801 gcgctggcag gcggttatat cgggcatctg ggggattatt cgaccggtgc ggcacaggcg
     4861 atcatgccgt atgtcgtggg tggcagcgaa gtttatcaac agcaaaccag ttggccgctg
     4921 gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa tacgctgaaa
     4981 attgcgtgga atgcatctga tgagcagggg ctttcttact tttccgcact gcgtgacagc
     5041 gggaaaaagc tgatctgtat tgatccaatg cgatcggaaa ccgtcgattt ctttggcgat
     5101 aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct ggggatcgcc
     5161 catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tgacgcgttg caccacaggt
     5221 tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagcgaa aaacgccgaa
     5281 tgggcggcag agatttgtgg ggttaacgca gcgaaaatcc gcgagctggc ggctcttttc
     5341 caccaaaaca cgaccatgct gatggctggc tggggaatgc agcgccaaca gtttggtgag
     5401 caaaaacact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg cacacccggc
     5461 ggcggttttg gtctttctta tcattttgcc aatggtggta accccacgcg gcgttctgcg
     5521 gtgctctctt ccatgcaggg cagcttgccg ggtggctgcg atgcggtgga taaaatccct
     5581 gttgcccgca ttgttgaagc actggaaaac cctggtggcg catatcaaca caacggtatg
     5641 aaccgacatt tcccggatat tcgttttatc tggtgggcgg gcggtgccaa ctttactcat
     5701 catcaggata ccaatcgtct gatccgtgcc tggcaaaaac cggagctggt ggtgatctct
     5761 gaatgcttct ggacggcggc ggcgaaacac gcggatatcg ttctgcctgc gactacctct
     5821 tttgagcgta atgatctcac catgaccggt gattacagta atcagcatct ggtgccgatg
     5881 aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt tgccgagttg
     5941 agtgaacgtt gggagaaggg cggctatgcg cggtttacgg aaggaaaaag tcagctgcaa
     6001 tggctggaaa cgttttataa cgttgcccgg cagcgcgggg caagccagca ggttgaattg
     6061 ccgccatttg ctgagttctg gcaagccaac cagttaattg agatgccgga aaacccggac
     6121 agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgctggcgca tccgttaaaa
     6181 accgccagcg gcaagattga aatcttctca cagcgtattg ccgattacgg ttacccggat
     6241 tgccctgggc atccaatgtg gctggagccg gacgaatggc agggcaatgc cgaaccagaa
     6301 cagttgcagg tactttctgc ccatccggcg caccgcctgc acagccagct gaattacagt
     6361 tctctgcgcg aattgtacgc ggtagcaaac cgtgagcctg tcaccattca tcctgacgat
     6421 gctcaggcgc gcggcataac cgaaggggat atggtgcggg tgtggaactc ccgtgggcag
     6481 atccttgccg gagcggtcat tagcgaggga attaaacctg gcgtgatttg cattcacgaa
     6541 ggggcatggc cggatctgga tttaaccgct gacggtattt gtaaaaacgg cgcagtgaac
     6601 gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg taatacggcg
     6661 ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt tgaaccaccg
     6721 gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa tcgacaatgt
     6781 gaaaaccctg cgcacggtaa aaatctatcg ccggttgatt tttttgataa acctccagca
     6841 tcagatgggg atagcgctgc tgcacatact gcatcagcgc cttaccaata ccgcgtctga
     6901 cggccttcgg tgcgacaaac attgctgcca gaaatcggcc ttccataatg ctgacaaaac
     6961 cgagaagctt accgtcctct tcccagaccc agttttgcgc gttggcaaga taggcatccc
     7021 gcaccagcgg aatgcagtca cgccagtaat tcgcttttat aaagggatgc ccccaggttg
     7081 tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct tcccgaatca
     7141 tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca gcccacatgc
     7201 ctgcataaag gagtaacaga ttgtggtgcc gacaaactta aaaccacgtt ttttcagtgc
     7261 cttagatagg gcgtcggagg cggacgtaga tgtgggaatt tcgctcaacg ttgtggcttg
     7321 tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg gttcgccgtt
     7381 ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt tccctcgatg
     7441 gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca ttgcggcaac
     7501 cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt tgaggacggt
     7561 gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca gttttttact
     7621 gtcagtttca ggcacgcccc actcattatc atggtaggca atataaagcg ggtcctgact
     7681 cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa aaatttcagc
     7741 cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac tatgcccgat
     7801 atccttcata caatgacaat aatattgccg aattcggctc ttccctggag tcgtgttaat
     7861 gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg tggtaatcag
     7921 tgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg atacgcaacc
     7981 cggactttcc acagagcgtt acacctggga tagtgatcat caacctgatt acaacgatat
     8041 tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca atctggcgga
     8101 agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc ctttatatga
     8161 acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca cctcgatgta
     8221 taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg ccagcgtgag
     8281 taccttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg cgcaaatcag
     8341 ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga gccggatgac
     8401 ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata tgttgctcaa
     8461 tcaaaatatt gccgcctatg ctgcgctaac tcaggcagaa aataccacta ataatagcga
     8521 ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca attgaaacca
     8581 ttgaataaca atagttgtgg cgatagtggg tgctaattta caaaataata aatttggtga
     8641 ataattgtcg cgtcattcat tcctgaacta aggcatttca ttccgttctg atggcatttc
     8701 atgccgtttt tccccaggca taaaatgcac ttcgttatgg ttgtcggcag agatttttcc
     8761 tttttattac tgcaggaata ctgccatgac accttcaaat tatcagcgta cccgctggct
     8821 gacactcatc ggtactatca ttacccagtt tgcgctgggg tcggtttata cctggagcct
     8881 gtttaatggc gcgctttctg ccaagctgga tgcgccggta agccaggtcg ctttctcttt
     8941 cggcttgtta agtctggggc tggcaatttc gtcttctgtt gcgggcaagt tgcaggaacg
     9001 ttttggcgtt aaacgcgtca ccatggcttc cggcattttg ctgggattag gcttcttcct
     9061 gaccgcgcat tccaacaacc tgatgatgct gtggttaagc gccggtgtgc tggtcggtct
     9121 ggcggatggc gcgggttacc tgctgacgct ctctaactgc gtgaagtggt tcccgg
//
LOCUS       DAZKIV010000166         8872 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZKIV010000166 DAZKIV010000000
VERSION     DAZKIV010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8872
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.166"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1082
                     /gene="emrK"
                     /locus_tag="ACHOT6_004033"
     CDS             <1..1082
                     /gene="emrK"
                     /locus_tag="ACHOT6_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFP9949174.1"
                     /translation="SGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVNHKD
                     TNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARIQYQ
                     QSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALVMNTPLNR
                     QPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPARQMW
                     VNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNATGN
                     WIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMPAYT
                     SKALVIDTSPIEKEISNIISHNGQL"
     gene            1082..2620
                     /gene="emrY"
                     /locus_tag="ACHOT6_004034"
     CDS             1082..2620
                     /gene="emrY"
                     /locus_tag="ACHOT6_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFP9949175.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2728..4056)
                     /gene="dsdA"
                     /locus_tag="ACHOT6_004035"
     CDS             complement(2728..4056)
                     /gene="dsdA"
                     /locus_tag="ACHOT6_004035"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFP9949176.1"
                     /translation="MENAKMNSLITQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4074..5411)
                     /gene="dsdX"
                     /locus_tag="ACHOT6_004036"
     CDS             complement(4074..5411)
                     /gene="dsdX"
                     /locus_tag="ACHOT6_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
                     /protein_id="HFP9949177.1"
                     /translation="MHSQIWVVSTLLISIVLIVLTIVKFKFHPFLALLLASFFVGTMM
                     GMGPLDMVNAIESGIGGTLGFLAAVIGLGTILGKMMEVSGAAERIGLTLQRCRWLSAD
                     VIMVLVGLICGITLFVEVGVVLLIPLAFSIAKKTNTSLLKLAIPLCTALMAVHCVVPP
                     HPAALYVANKLGADIGSVIVYGLLVGLMASLIGGPLFLKFLGQRLPFKPVPTEFADLK
                     VRDEKTLPSLGATLFTILLPIALMLVKTIAELNMARESGLYILVEFIGNPITAMFIAV
                     FVAYYVLGIRQHMSMGTMLTHTENGFGSIANILLIIGAGGAFNAILKSSSLADTLAVI
                     LSNMHMHPILLAWLVALILHAAVGSATVAMMGATAIVAPMLPLYPDISPEIIAIAIGS
                     GAIGCTIVTDSLFWLVKQYCGATLNETFKYYTTATFIASVVALAGTFLLSFII"
     gene            5629..6564
                     /gene="dsdC"
                     /locus_tag="ACHOT6_004037"
     CDS             5629..6564
                     /gene="dsdC"
                     /locus_tag="ACHOT6_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DsdC"
                     /protein_id="HFP9949178.1"
                     /translation="MEPLREIRNRLLNGWQLSKMHTFEVAARHQSFALAAEELSLSPS
                     AVSHRINQLEEELGIQLFVRSHRKVELTHEGKRVYWALKSSLDTLNQEILDIKNQELS
                     GTLTLYSRPSIAQCWLVPALGDFTRRYPSISLTVLTGNDNVNLQRAGIDLAIYFDDAP
                     SAQLTHHFLMDEEILPVCSPEYAQRHALTNTVINLCHCTLLHDRQAWSNDSGTDEWHS
                     WAQHYAVNLPTSSGIGFDRSDLAVIAAMNHIGVAMGRKRLVQKRLASGELVAPFGDMT
                     VKCHQHYYITTLPGRQWPKIEAFITWLREQVKTTS"
     gene            complement(6660..6734)
                     /locus_tag="ACHOT6_004038"
     tRNA            complement(6660..6734)
                     /locus_tag="ACHOT6_004038"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6699..6701),aa:Arg,seq:cct)
     gene            complement(6810..7742)
                     /locus_tag="ACHOT6_004039"
     CDS             complement(6810..7742)
                     /locus_tag="ACHOT6_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFP9949179.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            8036..8791
                     /gene="mlaA"
                     /locus_tag="ACHOT6_004040"
     CDS             8036..8791
                     /gene="mlaA"
                     /locus_tag="ACHOT6_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFP9949180.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYFQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         2359 a         2063 c         2091 g         2359 t
ORIGIN      
        1 tttcaggtgc ctatgcctat tggtcaatgg aattagaaga catgattagt acagatgacg
       61 cctatgtcac ggggaatgca gatccaattt ctgcacaagt ctcaggtagt gtcactgtcg
      121 ttaatcataa agatacgaac tacgttcgac aaggtgacat tttagtttca ctggataaaa
      181 ctgatgccac tatcgcactc aataaagcta aaaataatct ggcaaatatt gttcggcaaa
      241 cgaataaact atacttacag gataaacaat acagtgccga agtcgcttca gcacgtattc
      301 agtatcaaca atctttagaa gattataacc gtcgagtgcc gttagcgaag cagggggtta
      361 tttcaaaaga aacgctggag cataccaaag atacgttaat aagtagcaaa gcggcattga
      421 atgccgctat ccaggcttat aaagcgaata aagctttagt aatgaacaca ccattaaacc
      481 gtcagccaca agtcgttgaa gcggcggatg caactaaaga agcctggttg gcgcttaaac
      541 gtacggatat taagagtccg gttaccggct atattgccca gagaagtgtt caggtcggcg
      601 aaacagtgag ccccggacaa tcgttaatgg ctgtcgtacc ggcacgtcaa atgtgggtta
      661 atgccaactt taaagaaaca caactcacgg atgtgcggat tggtcaatcg gtcaatatta
      721 tcagcgatct ttatggtgaa aatgttgtgt ttcatggtcg ggtgacaggg atcaatatgg
      781 gaaccggcaa tgcgttctcc ttattacctg cacaaaatgc gacagggaac tggatcaaaa
      841 tcgttcagcg tgtaccggtt gaagtttctc ttgatccaaa agaactcatg gaacatccct
      901 tgcgtattgg tttatcgatg acagcaacta ttgatacgaa gaacgaagac attgccgaga
      961 tgcctgagct ggcttcaacc gtgacctcca tgccggctta taccagtaag gctttagtta
     1021 tcgataccag tccgatagaa aaagaaatta gcaacattat ttcgcataat ggacaacttt
     1081 aatggcaatc actaaatcaa ctccggcacc attaaccggt gggacgttat ggtgcgtcac
     1141 tattgcattg tcattagcga catttatgca aatgttggat tccactattt ctaacgtcgc
     1201 aataccgaca atatctggct ttctgggagc atcaacagac gaaggcacct gggttatcac
     1261 ctcgtttggt gtagcaaatg ccattgcgat ccctgttact ggcaggttgg cacaaagaat
     1321 aggcgaatta agattatttt tactttcagt aacctttttt tcgctgtctt cattaatgtg
     1381 tagcctatcg accaatcttg atgtgctgat attttttaga gtcgttcagg ggttaatggc
     1441 ggggccgtta attccactgt cacagagttt attattaagg aattatccgc cagaaaaaag
     1501 aacatttgct ctggcattat ggtcaatgac cgtgattatc gctccgatat gtgggccgat
     1561 attgggcggt tatatttgtg ataactttag ctggggttgg atatttttaa tcaatgtccc
     1621 tatggggatt atcgtcctga cattatgctt aaccttactt aaaggaagag aaactgagac
     1681 ttcaccggtc aaaatgaatc taccaggact gaccctgtta gtgctcggtg ttggtggctt
     1741 gcaaattatg cttgataaag ggcgcgatct ggattggttc aactcgagta caataataat
     1801 attaacagta gtatcagtta tttctctgat ctctttagtc atttgggagt cgacctcaga
     1861 gaatccgatt cttgatctca gtttgtttaa gtctcgtaat ttcaccattg gtattgtgag
     1921 tatcacctgc gcgtatttat tttactctgg agcgatcgtc cttatgccgc agttactcca
     1981 ggaaacgatg gggtataatg cgatatgggc cgggcttgct tatgcgccca tcggcatcat
     2041 gccactattg atttcacctt tgataggacg ttatggcaat aaaatagaca tgcgggtgtt
     2101 agtgacattc agttttttga tgtatgcggt ttgctattac tggcgttctg tgacatttat
     2161 gccaacgatt gattttacag gcatcatttt gccacagttt ttccagggat tcgccgttgc
     2221 ctgtttcttt ttacccttaa caacgatttc gttttcaggc ttgccagata ataaatttgc
     2281 caatgcctcg agtatgagta atttttttcg taccttgtca ggatcagttg gtacgtcgtt
     2341 gacaatgacg ctgtggggac gacgcgaatc gttacaccat agtcagttga cagcaaccat
     2401 cgatcaattt aaccccgtgt ttaattcatc gtcacaaatt atggataaat actatggttc
     2461 gctttcagga gttcttaatg aaattaataa tgaaataacc cagcagtcac tttctatttc
     2521 tgcaaatgag attttccgta tggcggctat tgcttttatc ttacttacgg ttttggtttg
     2581 gtttgcgaaa ccgccgttta cagcgaaagg cgttgggtga tgataaaagg agggggttat
     2641 agcgggtaag accggggatc ttaagtctgc gccatcggca gcatcgctca cccagggaaa
     2701 ggattgcgat gctgcgttga aacgttatta acggcctttt gccagatatt gattcatctc
     2761 ttcttccggc accattccac ctcccgtcgc ccacaccaga tgagtggcat tacgcaattg
     2821 ttctgcgctg aaaccgtgca tctgttggta acttactgat gcacacacgc gctgaggtcc
     2881 ggccataccc gccagtgctg aaggttcaag acgaatacct tcttcctgcg ccagccagcc
     2941 aagcatgtca tacatggttt gatcgctaag ggtatagaag ccatccagca gacgctccat
     3001 tgcccgcccg acaaagcctg atgcgcgacc aactgcaagg ccatccgctg cggtaaggtt
     3061 gtcgatacca atatcctgaa cagaaatctg atcgtgtaat cctgtatgga cgcctaacaa
     3121 catacaaggg gagtgcgttg gttcggcaaa aaagcagtga acatgatcgc caaacgccag
     3181 tttaagcccg aatgcgacgc caccaggacc accgccaaca ccacacggca gatagacaaa
     3241 cagagggtta tcagcatcga cgatacggcc ttgctgggca aattgcgctt taagacgctg
     3301 gccagcgacg gaatacccaa ggaacaacgt gcgggaattt tcgtcatcaa taaagaaaca
     3361 gttcgggtca gactgcgctg ctttacgtcc ttcctcgacg gcaacaccat aatcttgctc
     3421 atattccacg accgtaacgc catggctgcg cagtttcgct tttttccatg cccgggcatc
     3481 agcagacata tgaactgtca ccttaaagcc aatgcgggcg ctcataatgc cgattgataa
     3541 ccccagattt ccggttgagc ccacagcaat gctgtattgg ctaaagaact gtttaaactc
     3601 cggagaaagc agtttgctgt agtcatcatc aagcgtcagc aaccccgctt ccagagccag
     3661 tttttctgcg tgtgccagga cttcataaat cccgccgcgt gcttttatgg agccggaaat
     3721 gggcaaatgg ctatcttttt tcagtaacag ttgcccgctg atcggttgct gatattcttt
     3781 ttccagccgt ttttgcatag ctggaatggc aaccagttct gattcaataa tccccccagt
     3841 ggcagcagtt tcaggaaatg cttttgccag atagggtgca aaacgggata agcgcgcatg
     3901 ggcgtcctga acatcctgtt cggtcaggcc aacataaggt aaaccttcag ccaatgaggt
     3961 cgtgccagga ttaaaccagg tggtttcttt aagagcaacc agatccttta ccaacggata
     4021 ctgggtgatg agcgagttca ttttagcgtt ttccatagta cgtctctttg cgcttagatg
     4081 ataaatgaca gcaggaatgt gcccgccaga gcgacgactg aagcgataaa tgtcgctgtc
     4141 gtatagtatt taaatgtttc attgagcgta gcgccgcaat attgcttcac tagccagaaa
     4201 agcgagtccg taacgatcgt gcagccaatt gcacctgaac cgatagcaat cgcaataatt
     4261 tccgggctga tgtcgggata cagcggcagc atgggtgcaa caattgccgt tgcccccatc
     4321 attgccacgg tagcggagcc cactgccgca tgcagaataa gagccactaa ccaggccaga
     4381 agaatcgggt gcatatgcat attggagaga ataactgcca gcgtatcagc gagactgctg
     4441 ctttttaaaa tggcgttgaa tgcgcctccg gccccgataa tcagcaaaat attagcaata
     4501 gaaccgaagc cattttccgt atgtgtgagc atcgtcccca tgctcatatg ctggcgtata
     4561 cccaacacat aataggcgac aaacacggcg ataaacatgg cagtgatagg gttgccaata
     4621 aactcaacca agatatacaa accactctca cgcgccatat tcaattcggc aatcgtttta
     4681 accaacatca gcgcaatggg tagcagtatg gtgaataacg ttgcgcctaa tgacggtagt
     4741 gttttttcat cgcgaacttt gagatctgca aactctgtgg gtacaggttt aaagggcagt
     4801 cgttgaccca gaaatttaag gaaaagtggg ccaccgatca gtgatgccat cagcccaacc
     4861 agcaaaccgt agacgatcac cgaaccgata tctgcgccca gcttattggc aacatataaa
     4921 gcagccggat gtggaggaac cacgcagtgc actgccatca atgcggtaca tagcggaatg
     4981 gcaagcttta ataatgaggt attggttttt ttggcaattg aaaaagccag aggaatcaat
     5041 agcacgacgc ccacttcaac aaacagcgtg atgccacaaa tcaggccaac cagcaccata
     5101 atgacatcag ctgaaagcca gcggcagcgt tgaagtgtca gaccaattct ttctgcggcc
     5161 ccggatactt ccatcatttt tcccagtatc gtgccaaggc cgataaccgc tgcgaggaac
     5221 cccagcgttc cgccaattcc actttcaata gcatttacca tatccagtgg ccccatgccc
     5281 atcatcgttc ccacgaagaa gctggccagc aacagcgcca gaaacgggtg gaatttgaac
     5341 ttcacgatgg tcagtacaat taacacgatg ctgataagca gcgtgctcac aacccagatt
     5401 tgagagtgca tatctcacct tcccctgtga ttaacctgat gatattggac aaaaaaacaa
     5461 cgcaggctga caaacgataa aatttgcctt tcacatgagg taaattgcat caatggagtg
     5521 atttacatct aaaaatgaag gtgaattgag atatggttca ctttagctca tctcatagct
     5581 gaaattttgc cgctattttt ttacacttaa gcgaaaaatg aggtgattat ggaacccctt
     5641 cgtgaaataa gaaatcggct gcttaacggc tggcaattat caaaaatgca tacttttgaa
     5701 gtggctgcca ggcatcagtc cttcgccctg gcggcagagg aattgtcgct gagccccagt
     5761 gcggtaagtc accgtatcaa tcagctggaa gaagaattgg gcattcagtt gtttgttcgt
     5821 tcccatcgca aagtggaatt aacgcacgag gggaaacgtg tttattgggc gctaaaatcg
     5881 tcgctggata ccctgaacca agaaattctt gatatcaaaa atcaggagtt atcgggaacg
     5941 ttaactctgt attcccggcc ctctatcgcc caatgctggt tggtgcccgc attaggtgac
     6001 tttacacgcc ggtatccgtc tatttcgctc accgtgctca ctggtaatga caacgtcaat
     6061 ctgcaacgtg ccggaatcga tttggcgatc tactttgatg atgcgccgtc agcgcaactg
     6121 actcatcact ttctgatgga tgaagaaatc ctgccagtat gtagcccgga atacgctcaa
     6181 agacatgctt taaccaacac ggtaattaac ctgtgtcact gtacgttgct ccatgacaga
     6241 caggcatgga gcaacgactc cggtacggat gaatggcata gttgggcgca acattatgcg
     6301 gttaatttgc cgacatcttc tggaattggc tttgatcgtt ctgatttagc tgttattgcc
     6361 gcgatgaatc atattggggt ggcgatggga agaaaacgcc tggtacaaaa aaggcttgcc
     6421 agtggtgagc tcgtcgcgcc gtttggcgat atgacggtga aatgccatca gcattattac
     6481 atcaccacat taccgggcag gcagtggcca aaaattgaag cgtttattac ctggctaaga
     6541 gaacaggtaa aaaccacttc ataatgaata aaaccacgag ttcctggtgt gccttattcg
     6601 gcctgctagt tcgaatttaa tttacggttt ttatgtggtt ttagggataa acacaaggat
     6661 ggtgtcccct gcaggaatcg aacctgcaat tagcccttag gaggggctcg ttatatccat
     6721 ttaactaaga ggacaatgcg gcatgagtat acccgctaat ggactgcggg gtaagtacgc
     6781 tgccgctcga ttgcttaaac cctcgccatt tatgctgggt ttttatcatt tttcttaatg
     6841 ttttccgcac gttctgcttt ttggcgtgct tctgctttac gcttgttgct catgtcgtta
     6901 cgaatctgtg catgactcat taacgcgaag ataaaggtgc cgccgcagat gttccccgct
     6961 aaagtaggta gtgcgaaggg ccagatgaaa tcgctccagt gcagcgtgcc gttaaacacc
     7021 agatagagga tttcaacaga accgacaacg atgtgggtgg tgtcacccag tgcaataagc
     7081 caggtcatca atataatcac cacaatcttt gccgcacccg cagcaggaaa catccaaacc
     7141 atagtggcga tcagccagcc ggaaatgatc gcgttggcaa acatctcgct gggggtgttc
     7201 ttcatcacat ccatgccgat tttgacaaat gcatcgcgag tttcttcatt gaagataggc
     7261 atatattcaa atgcccatgc agcaatacct gtcccgagaa tattacccag cagcacgacg
     7321 ccccataatc gcataagtaa gccgacgttg ctcattgtcg gtttttgcat gacgggtagt
     7381 accgcagtca cggtgttttc ggtaaataat tgctggcggg ccataatgac gataataaaa
     7441 ccaaaggtat aaccgagatt ctccagtaag aagctgcctg gcactccttc cagttcgaca
     7501 tgaaatatcc cttttgccag tagcgaagcg cccatcgaca gacccgccgc aatggctgac
     7561 cacagtagcg ccattgcgtc gcgttccagc tctttttcac catcctggcg gatatgctca
     7621 tgaattgcca tcgcccggga ggggagtcgg tcttcatcta tttctatttt tttgccgcgc
     7681 tctttttctt cgctctcaac ttcaatttcg tcgctgtgtt gatcaatttt gtcgttgtcc
     7741 atggtctctt ctcttgaatt agcacgtata gctaaagcgt agcggctttt ttgctcgcaa
     7801 ctggcgggag ttactctgaa aatgtagaaa aggctgcgtt tgcctttttc tgtttctata
     7861 gaatcaagta gcctacaggg cggcgattac caggctatga tcaaatcagc aaatcagggc
     7921 gtctggacat cagttgacgt gctgttacaa tcgcccacac ctaaacaggc ggatacggta
     7981 tcgttccgtc atggatggca aactgcataa gccataaaaa aacagggaga catttatgaa
     8041 gcttcgcctg tcggcgcttg ctctgggaac tacgcttctg gtggggtgtg cgagttccgg
     8101 tacagatcag caagggcgtt ctgacccgtt agaagggttc aaccgcacca tgtacaactt
     8161 caacttcaat gtattagacc cgtatattgt tcgaccggtc gctgtcgcct ggcgtgatta
     8221 tgttccgcaa ccggcgcgta acggtttgag caactttact ggcaaccttg aagaacctgc
     8281 ggtgatggtt aactacttct tgcagggcga cccttatcag gggatggtcc actttacccg
     8341 ctttttcctg aacaccattt tggggatggg cggttttatt gatgttgcag ggatggcgaa
     8401 cccgaaactg caacggactg aacctcaccg cttcggtagt acgcttggtc attatggcgt
     8461 gggttatggg ccttacttcc agttaccgtt ctacggtagc ttcacgctgc gtgatgacgg
     8521 tggtgatatg gcggatggtc tttacccggt tctttcctgg ctgacctggc cgatgtctgt
     8581 gggtaaatgg acgcttgaag ggatcgaaac ccgcgctcag ctgctggatt ccgatggtct
     8641 gctgcgtcag tcgtccgatc cttatattat ggtgcgcgaa gcgtacttcc agcgtcatga
     8701 tttcatcgct aatggcggcg aactcaaacc gcaggaaaat ccgaacgcgc aagcgattca
     8761 ggatgattta aaagatatcg attctgaata agaaacaaat aaaaaaggtg agtcgcaata
     8821 ctcacctttt ttgttgttat ccctcaatta ccacaagtaa aaggagataa ac
//
LOCUS       DAZKIV010000167         8772 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZKIV010000167 DAZKIV010000000
VERSION     DAZKIV010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.167"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..159
                     /locus_tag="ACHOT6_004041"
     CDS             <1..159
                     /locus_tag="ACHOT6_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9949181.1"
                     /translation="RTIVELGKAIDFDARTAIPFEGERHNALDDARYQAKYVSVIWQK
                     LIPSQADS"
     gene            232..483
                     /locus_tag="ACHOT6_004042"
     CDS             232..483
                     /locus_tag="ACHOT6_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFP9949182.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            503..1798
                     /locus_tag="ACHOT6_004043"
     CDS             503..1798
                     /locus_tag="ACHOT6_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFP9949183.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLIKPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLLNTQLSEFAPTMPHNEAMKS"
     gene            complement(1986..3005)
                     /gene="rspB"
                     /locus_tag="ACHOT6_004044"
     CDS             complement(1986..3005)
                     /gene="rspB"
                     /locus_tag="ACHOT6_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFP9949184.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(3017..4231)
                     /gene="rspA"
                     /locus_tag="ACHOT6_004045"
     CDS             complement(3017..4231)
                     /gene="rspA"
                     /locus_tag="ACHOT6_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFP9949185.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHNIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4437..4763)
                     /gene="ynfA"
                     /locus_tag="ACHOT6_004046"
     CDS             complement(4437..4763)
                     /gene="ynfA"
                     /locus_tag="ACHOT6_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFP9949186.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4898..5239
                     /gene="ynfB"
                     /locus_tag="ACHOT6_004047"
     CDS             4898..5239
                     /gene="ynfB"
                     /locus_tag="ACHOT6_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFP9949187.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5274..5834
                     /gene="speG"
                     /locus_tag="ACHOT6_004048"
     CDS             5274..5834
                     /gene="speG"
                     /locus_tag="ACHOT6_004048"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFP9949188.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5837..6547)
                     /gene="ynfC"
                     /locus_tag="ACHOT6_004049"
     CDS             complement(5837..6547)
                     /gene="ynfC"
                     /locus_tag="ACHOT6_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFP9949189.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            6655..6960
                     /gene="ynfD"
                     /locus_tag="ACHOT6_004050"
     CDS             6655..6960
                     /gene="ynfD"
                     /locus_tag="ACHOT6_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFP9949190.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7159..>8772
                     /locus_tag="ACHOT6_004051"
     CDS             7159..>8772
                     /locus_tag="ACHOT6_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO/selenate family reductase complex A
                     subunit"
                     /protein_id="HFP9949191.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGTRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNSHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMG"
BASE COUNT         2480 a         2128 c         1995 g         2169 t
ORIGIN      
        1 cgcacaatcg ttgagctggg gaaagccata gacttcgatg ccagaacggc tattccattc
       61 gaaggtgagc gccataatgc acttgatgac gcccgttacc aggcaaaata cgtttcagtt
      121 atctggcaaa aactgatccc gagtcaggct gattcttaat gttcaactgt cgccggttgt
      181 gactggtatt ctgcaaccgg cgctcgtctg atgtaagaga taaagaaagc gatgagcgaa
      241 gtaatcatga ttgtctctcc cggcaaatgg gtatccgaag agcagttaat tgcgctgaaa
      301 ggaataaaaa aaggtacgtt aaaaaaggcc cgggaaaaat cgtttatgga aggaagggaa
      361 tataagcatg tcgctcatga cggtatgcca tgggataaca gtccatgctt ttacaacctg
      421 gaagaaattg atcgctggat tgagcgccag gcatctgcaa gaccaagacg tcatcttgct
      481 tgactaaaag caacacgaac caatgagaga agctgaaatg aaatatccga caggcgtgga
      541 aaaccatgga gggaaattac gtatctggtt tgtttataaa ggtgtaagag tccgggaaaa
      601 tcttggggtt cctgacacag caaaaaacag gcgcattgca ggtgagctac gcgcctctgt
      661 ttgttacgca ataaaaactg gcgctttcga ctatgcaaaa cagtttcccg cctcacacaa
      721 tctggaaaaa tttggtgagg cccgacaaga tttaaccata aaagaactgg ctgaaaaatt
      781 tctggcactg aaagaaactg aagtcgcaaa aacgtcactc aacacgtacc gtgccgtcat
      841 caaaaatatt ctgagcataa tcggtgaaaa aaatcttgca tcatcgatta ataaagaaaa
      901 attgctggcg gtacgtaaag agttactgac tggataccag atccccaaaa gtaactatat
      961 tgttacacaa cccgggagat cggctgttac cgtaaataat tacatgacaa atctttacgc
     1021 cgtgttccag tttggtgttg ataacggtta tctggcagac aatccattta aggggatctc
     1081 accattaaag gagtcgagaa ccatcccgga tccactttcg cgggaggagt ttatccgcct
     1141 tattgacgcg tgcagaaatc agcaagccaa aaatttatgg tgtgtttccg tttatactgg
     1201 gattcgccct ggtgaactct gtgcgcttgg atgggaagat atagatctga aaaatggaac
     1261 aatgataatc agaagaaatc tggcaaaaga ccgttttaca gtaccaaaaa cacaggcggg
     1321 aaccaatcgt gtgattcacc ttattaagcc cgcaatcgac gctctccgga gtcagatggc
     1381 actaacgaga ctgagcaaag agcatatcat cgatgttcac ctcagagaat tcggcagaac
     1441 agagaaacaa aaatgtacct ttgtttttca acctgaagtg tcagcgaaag taaaaaatta
     1501 tggcgaccat tttaccgttg actcaataag gcagatgtgg gacgcagcgg taaagcgtgc
     1561 cggaatccgc catcgcaaat cgtatcagtc gagacacact tatgcctgct ggtcgttaac
     1621 agcaggagct aacccggcat tcattgcaaa ccagatgggc catgcagatg cgcaaatggt
     1681 gtttcaggtg tacgggaaat ggatgtcaga aaataataat gcgcaggtaa cgctgttaaa
     1741 tacacagtta agcgagtttg ccccaaccat gccccataac gaagcgatga aaagttaatt
     1801 ttttatttat caattagtta gattgaatga ctcttgaaat ccataaattc aagcgcagtg
     1861 cccagccatc ccgatactgc tgctttcacc aaatccttag tgcttctttc atgtttttct
     1921 attgtcataa tggttatctc taaaaaagag gtaagatgcg tactacttac tcgccgttat
     1981 tggtattatt cagaaaaagt gagtaagact ttgcagcaat gtttttgatc ctgttcaaat
     2041 aaactaatgg catcagcaac atgctggaaa tcaaacgtat gggtaattaa tttttctggt
     2101 ttaattaacc ctttacttaa ccagtcgata acaaccggaa atttatttgc atttaagcgt
     2161 gaagagaaaa tagagagttc ttttccggta attccttgct gaatcacttc agacggttca
     2221 ctggagaagc ccatcaatac aatacgtgcc gctggagaag ccagcgttac ggcctctttc
     2281 aggatagaag gatgacaagc cgcatcgata attaatgtcg gcttgatgcc tttttcagcg
     2341 aaaatctcgc caagcggtgt ctggctgtta ttaatcgccc agtctgcccc gctctctttc
     2401 gctttttcca gtcgttcatc aatgcgatcg gcaacaatca catttttaac gttatagacg
     2461 ccttttaata cctgaacgat cgtcaggccg attggaccgg caccataaac cagaacggta
     2521 tcattttcag tcggttgacc atgaccggta acgttagccg caatggtaaa aggttcgatc
     2581 atcaccgcat attgatcggc cactgcttca ggaattttcc acgcattttt tgccggaacc
     2641 acggcatatt cactgaaacc accgtcagcg tgcacaccta atacagcaag tgtcgtacaa
     2701 acgttcggct tacctataga gcacggatag caatgcccac agctgaccac cggatcgaca
     2761 gcaacgcgtt caccgactct ggcgctttcc acaccttcac ccaccgcatc aatgacgcca
     2821 aagaattcat gaccaatgac gcgcggatat ttcgcaaaag gattatgccc acggtaaata
     2881 tggctatctg aaccacaaat tccggcaagt ttcactttta ctcgtacttc acccgctgac
     2941 ggggtgggta tttcacgttc gataatcgac agttgattcg gtttttcaat taaaatgctt
     3001 ttcattacct tactccttac cagttccaca gcgtgccatc ttccagacgt gcgactggca
     3061 gataagcagg ttcataggga tatttcgccg ccagcttttc atcgaattcg atgccaagac
     3121 ccggtttgtc tcccggatgc atatagccgt tatcgaaagt ccagttgtgc gggaagactt
     3181 cgagcatttg ttcggaataa cccatgtact cctggacacc gaaattgggg acccacagat
     3241 caaagtgcag cgccgcagcc atgcagactg gtgacaaatc ggaaggaccg tgtgagccag
     3301 tacgtacctg atacagcgaa gcaaaatcgg caatccggcg cataccggta attccgcctg
     3361 catgggtcag cgtggtgcgg atataatcga tgagttgctc ttcaatcagt tgtttgcagt
     3421 cccagatgct gttgaagact tcacccactg cgatgggtgt gacggtatgt tggcgaatga
     3481 gacggaagca ttcctggttt tccgcaggcg tcgggtcttc catccagaac atgcgataat
     3541 cttcaatgct tttaccaaag cgcgccgctt caataggcgt taagcgatgg tgcatgtcat
     3601 gcagcaaatg ttcattaaaa ccaaacttgt tacgtaccgc gtcaaacaat ttcggcatga
     3661 aatcgaggta tttctccgtc gaccacagct gctcttccgg ccactgtcct ttggttgcgg
     3721 gttcataagc cagaccttta cctttcgaca tgccgtaggt ggttttcata ccagggattc
     3781 cgcactgcac gcggatggct ttgaatccca gctcctgatg acgggcataa tcatccagag
     3841 cttcatcaat attgtgaccg gtggtatggc aataaaccat caccccttca cgagacgcgc
     3901 cgccgagtaa ctggtaaagc ggcatgttgg cagctttggc tttaatatcc cacagcgcca
     3961 tatcaaccgc tgaaatggcc gacatcgtaa ccggaccgcg acgccagtaa gcacctttat
     4021 agaaaaactg ccagatatct tcgatacggt gcgcatcgcg accaataagc tgcggacaaa
     4081 ggtgatcctg caaataagag gccacggaaa gctcacgtcc attgagggtg gcatccccaa
     4141 ggcccgtaat accgtcctca gtggtgattt ttaatgtgac gaaattacgc cccggacagg
     4201 taacaaaaac ttcagccttt acgatcttca tgttcgattc cttgcatcgc ttgtcgtgat
     4261 gcatgaaatc tacgcaactg agctactacc atacaagtat aaagatcgaa aaaagccgga
     4321 gtgatcacaa aaaaaggcgt atatttgcgc tgtgaatggt tgacaaaaga tgaaatagaa
     4381 taccttttgt cagctgacac ttcctcttat cttattgata aaatggattt atgttcctac
     4441 gtgcgccccc agcccgcaac aatgatcaac atgccgcaaa gcgcaatcaa cgcacccgtc
     4501 cagtcataaa gagtcagttt cacgccatcc acaacgcgca gccacatcaa cgccgtgcag
     4561 acataaacgc caccataagc cgcgtaaaca cgcccactcg ccgctggatg caacgttaac
     4621 aaccagacaa acagcgccag tgaaatcccc gccggaagca acagccagat actggcgttt
     4681 cgttttaacc acaaccaggg cagaaagcat ccaataattt cacacagcgc agtagcaaaa
     4741 aatagtaacg ttgttttaat catctttgtc acttattgac atcatgtata gttatagggc
     4801 gacataatat catcaatata aacaccctcc tggtacgttt tgcgttcgca gtggatggtg
     4861 tagaatcacc tttaatcatt catacaggga atgaattatg aaaatcactc tcagcaaacg
     4921 aatcggcctg ctcgcttttc tgctgccttg cgcactggca ctgagcacaa ctgttcatgc
     4981 cgaaactaac aaactggtga ttgagtctgg cgacagtgca caaagccgcc agcacgccgc
     5041 tatggaaaaa gagcaatgga atgacacgcg caatctgcgt cagaaagtga ataaacgcac
     5101 tgaaaaagag tgggataaag ccgacgccgc ttttgataac cgcgataaat gtgagcaaag
     5161 cgccaacatc aatgcctact gggagccaaa tactttgcgc tgcctggacc gtcgaactgg
     5221 ccgcgttatt accccctaac ctgttattga tttaaggaat gtaaggacac gttatgccaa
     5281 gcgcccacag tgttaagcta cgcccgctgg agcgtgaaga tttacgctat gtacatcaac
     5341 tcgacaataa cgccagtgtg atgcgttact ggtttgagga accctacgaa gcctttgttg
     5401 aactctctga tctgtatgat aagcatattc acgatcagag cgaacgacgc tttgtggtgg
     5461 aatgtgacgg cgaaaaagcc ggtctggtgg agctggtgga aattaaccat gtacatcgcc
     5521 gggcagaatt tcagataatt atctccccgg agtatcaggg gaaaggtctg gcaacccgtg
     5581 ccgccaaatt agcaatggac tatggcttca ccgttctcaa tctctataag ctgtatctga
     5641 tcgtcgataa agagaatgaa aaagcgattc acatttaccg caagcttggc ttttcggttg
     5701 aaggtgaatt gatgcacgag ttctttatta atggtcaata tcgtaatgcc attcgcatgt
     5761 gtatattcca gcatcagtat ctggcagagc acaaaacacc gggtcagact ctcctgaagc
     5821 cgaccgcaca atagcattaa taataatcga tcgtattttt gatggtgtaa acccgttcga
     5881 cggcgggttt tactccttca tcaacaatga tcagctgaca gtccaccgga ttggcgtgac
     5941 tgtcatattc acagctctgt tttacattac caactcgttg attattcagt aaagtaaccg
     6001 ctgtgtaatc taattttttg atcgggtccg ttgatggcgt ggcgctgaca gataatgttt
     6061 tgtcgttact tttcgtggtt ttacccagtg gataaccctg atcatcatag cgatattcca
     6121 tctgcatttg tttgctgctg gctttaatca cgaagccatt atcatcggtt tcccaactca
     6181 ccccggcaga aggtaattct gctagctggc attttccctg taaacgtact ctcttctcca
     6241 gcgtctcggc atcacggtaa taattggcgt ccagtaccag agcgaccacg gtattatttt
     6301 ccagatccag taattcgagt gaatcaaaac agccttcttc cgacaaagtc ccagaaacac
     6361 gtttcgtcac ttcaccttgc tcatccatta atgtctgagt gaaatctttt accggaccac
     6421 gcagcggatc aaaatcgaat tcattagaga aactggccat ctcaggggta aatgaaagtg
     6481 ttacctctgt gcggtcacat cctgtgagga atatcgcgag taagcatggt agtaatttgt
     6541 atttcacaac agtcaccaga gagtagagat gattctcaat catagtagca aatacagtac
     6601 tttacacgtt aaatgctatg cttaaagaag ttatcttcgc gtaaggagct tatgatgaaa
     6661 ctctcaacct gctgtgccgc gcttctgctc gccctcgcct cacccgcggt actcgccgcg
     6721 ccgggatcct gtgagcgcat acagagcgat atatcacagc gcattatcaa taatggcgta
     6781 cccgaaagca gcttcacatt aagtattgta cccaatgacc aggttgatca gcctgattcc
     6841 caggtcgtcg ggcattgtgc taatgatacg cataaaattc tctacacccg caccaccagc
     6901 ggtaacgtct ctgctcccgc gcagtctacc caggatggcg cgcctgccga accgcagtaa
     6961 tacattatcg actgaacgcc ggatacgaca catccggcga tttgaacata caacataatc
     7021 ccaccttatt actcataccc ttctattgat atggattaat aattcttaac ccaaaatggg
     7081 tagactccct ctattgttag cgcgctaaat attcaatata taaactttta tatgacgata
     7141 aagaacaggg agtgagttat gtccaaaaat gaacgaatgg tgggcatcag ccgcagaaca
     7201 ctcgttaaat ctaccgcgat aggttctctg gcgctggctg caggcggttt ttctttgccg
     7261 tttaccctgc gcagtgcagc agcagcggta caacaggccc gcgaaaaagt ggtctggggt
     7321 gcctgttccg tcaactgtgg tagccgctgt gcacttcgtc tacatgttaa agataatgaa
     7381 gtgacctggg tggaaactga caataccggc agcgatgagt acggcaacca tcaggtacgc
     7441 gcctgtttgc gcggtcgctc catccgccgg cgtattaatc atcccgatcg cttgaattac
     7501 ccaatgaaac gcgtgggtac acgcggtgaa ggcaaattcg aacggattag ctgggatgaa
     7561 gccctggata ctatcgccag tagcctgaaa aaaaccgtcg aacaatatgg caatgaggct
     7621 gtatatattc agtactcttc ggggattgtt ggcggcaata tgacccgctc ttcgccatca
     7681 gcctcggcgg tcaaacgcct gatgaactgc tacggcggtt cactcaatca gtatggctcc
     7741 tacagcaccg cgcaaatttc ctgtgccatg ccctacacct acggcagtaa tgatggcaac
     7801 agcaccacgg atattgaaaa cagcaagctg gtcgtgatgt ttggtaacaa cccggcagaa
     7861 acccgcatga gcggcggtgg catcacttat cttcttgaaa aagcgcgcga gaaatcgaac
     7921 gccaaaatga ttgttatcga tccgcgatat accgatacgg ctgctggtcg tgaagatgaa
     7981 tggctcccta ttcgcccggg caccgatgcc gcgctggttg cgggtattgc ctgggtattg
     8041 attaacgaaa atctcgttga tcaacctttt ctcgataaat actgcgtcgg ctatgacgaa
     8101 aaaaccttac ccgcagatgc acccaaaaat agtcactata aagcctatat tcttggtgaa
     8161 ggtgatgata acacagcgaa aacgccgcag tgggcttcgc aaattaccgg tatcccggta
     8221 gaccgtatca tcaaactggc gcgtgaaatt ggcacagcaa aacccgctta tatctgccag
     8281 ggctgggggc cgcaacgcca ggcaaacggc gaactgactg cacgcgctat tgctatgtta
     8341 cctattttga cgggcaatgt cggcatcagc ggcggaaata gtggcgcgcg tgaatcgacc
     8401 tataccatta ccatagaacg cctgccggtg ctggataatc cggtcaaaac gtcaatctcc
     8461 tgcttcagct ggacagatgc tatcgatcat ggtccgcaaa tgacggcaat ccgcgacggc
     8521 gtccgcggca aagataaact ggatgtgccc attaagttca tctggaacta cgcgggaaat
     8581 accctcgtta atcagcattc tgacatcaac aaaacgcatg aaattctgca ggacgaatcg
     8641 aaatgcgaaa tgattgtggt catcgaaaac tttatgacct catcggcaaa atatgccgac
     8701 attctgctgc cagacctgat gaccgttgag caggaagata ttattcctaa cgactacgcc
     8761 ggtaacatgg ga
//
LOCUS       DAZKIV010000168         8645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZKIV010000168 DAZKIV010000000
VERSION     DAZKIV010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.168"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1823
                     /gene="espL4"
                     /locus_tag="ACHOT6_004052"
     CDS             <1..1823
                     /gene="espL4"
                     /locus_tag="ACHOT6_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
                     /protein_id="HFP9949192.1"
                     /translation="LNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIVCRHIAAQYINDI
                     YQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGDFGLVMKAYF
                     DKMESNGISVMAAILLVDNHALTARLRIKNTTEGCTHYVVSVYDPNVTNDKIRIMSES
                     KEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGSVDEITPPLS
                     PATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSINLPGLYLAINYGNADIV
                     ETIFNSLSETGYEGLLSKKNLMHILEAKDKNGFSGLFLAISRKDKNVVTSILNVLPKL
                     AATHHLDNEQVYKFLSAKNRTSSHVLYHVMANGDADMLKIVLDALPLLIRTCHLTKEQ
                     VLDLLKAKDFYGCPGLYLAMQNGHSDIVKVILEALPSLAQEINISASDIVDLLTAKSL
                     ARDTGLFMAMQRGHMNVINTIFNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAI
                     QHKQQNVVETVYLALSDHARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAEL
                     ILNTLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(1792..3528)
                     /gene="aceK"
                     /locus_tag="ACHOT6_004053"
     CDS             complement(1792..3528)
                     /gene="aceK"
                     /locus_tag="ACHOT6_004053"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFP9949193.1"
                     /translation="MSRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3809..5113)
                     /gene="aceA"
                     /locus_tag="ACHOT6_004054"
     CDS             complement(3809..5113)
                     /gene="aceA"
                     /locus_tag="ACHOT6_004054"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFP9949194.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(5143..6744)
                     /gene="aceB"
                     /locus_tag="ACHOT6_004055"
     CDS             complement(5143..6744)
                     /gene="aceB"
                     /locus_tag="ACHOT6_004055"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFP9949195.1"
                     /translation="MTEQATTTDELAFIRPYGEHEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(7013..7942)
                     /gene="metA"
                     /locus_tag="ACHOT6_004056"
     CDS             complement(7013..7942)
                     /gene="metA"
                     /locus_tag="ACHOT6_004056"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFP9949196.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEFFRDVEAGLDPDVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            8099..8541
                     /locus_tag="ACHOT6_004057"
                     /pseudo
     CDS             8099..8541
                     /locus_tag="ACHOT6_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         2212 a         2114 c         2140 g         2179 t
ORIGIN      
        1 aattaaacct aaaatcagat agaaaagaaa aagaattccc aagaattaaa cttaatggtc
       61 aatgttattt tccggggcga ccccaaaacc gtatagtatg ccgacacatt gctgcacaat
      121 atattaatga tatttatcag aatgttgatt acaaacccca tcaagatgat tactcttcag
      181 ctgaaaaatt tctcactcac ttcaacaaaa aatgcaaaaa ccagactttg gcgttgattt
      241 ccagtcgtcc tgaggggcgt tgtgttgctg cctgtggtga tttcgggcta gttatgaagg
      301 catatttcga caagatggaa tcaaatggca tcagtgttat ggcagccata ttattggtgg
      361 ataaccatgc tttgacggcc cgactaagaa taaagaacac aactgaagga tgtacccatt
      421 acgtggtttc ggtttatgat ccaaatgtaa ctaacgataa aataagaatc atgagcgaaa
      481 gtaaagagga tattaaacac tattctctga tggattttat gaatgtagat tatagcctcc
      541 tgaaatggtc aaatgatcat gttattaacc aatctgttgc aataattcca gcacttccga
      601 aagaacagct attgatgtta aaaggatctg tggatgaaat aacccctcca ttatcacctg
      661 caacgatgaa tttgctaatg gcaattggtc agaatcacca acttacgcaa ctgatgattc
      721 agctccagaa aatgccagaa ctacatagaa cagaaatgtt gactgcctat aatagtatta
      781 acctccccgg tttatatttg gctataaatt atggcaatgc ggatatcgtt gagactattt
      841 tcaattcatt gtcagaaaca ggatatgaag ggttactctc gaaaaaaaat ctcatgcata
      901 ttctggaggc aaaagataaa aatggttttt ctggattatt tttagcgata tcacgtaagg
      961 ataaaaatgt tgtaacctcg attctgaacg tcttacctaa actggccgca acacatcatt
     1021 tagataatga acaggtgtat aaattcctga gtgccaaaaa tagaacgtcc agccatgttt
     1081 tataccatgt tatggcgaat ggtgatgccg acatgctgaa aattgttttg gacgcattac
     1141 ctttgttaat tcgcacatgt catttgacta aagaacaggt actcgatctc ctgaaggcaa
     1201 aggattttta tggttgccca ggactatacc tagcgatgca aaatggacat agcgatatcg
     1261 tgaaagttat tcttgaagca ttgcccagcc tggcccagga aattaacatt tcagcttccg
     1321 atattgtaga tcttctgacc gctaaaagtc ttgcgcgcga cacgggtttg tttatggcca
     1381 tgcagcgcgg acatatgaac gttattaata ctatttttaa cgcattaccc actctgttta
     1441 atacgtttaa attcgataaa aaaaatatga agcccctcct cctggcaaat aattctaatg
     1501 aatatcccgg tttgttttca gcgatacagc ataaacaaca aaacgttgta gagacggttt
     1561 atcttgcttt atctgaccat gcacgcctgt ttggatttac cgctgaagat attatggatt
     1621 tttggcaaca caaagcgcca caaaaatact ctgcctttga gttggctttt gaatttggtc
     1681 accgggttat tgctgaatta atccttaata cattaaataa gatggctgaa agctttggct
     1741 ttacggataa ccctcgatac attgcggaga aaaattatat ggaagcttta ctcaaaaaag
     1801 catctcccca taccgtacgc taaatctttg cctgcgccga tatgcataaa catcttccac
     1861 atgcccttca cgtatgcggt tttgtagtgc gcgccagtaa tcagcgcgga acaggtcggc
     1921 gtgcatctct tcaaacagcg gaccaatacg cgggtcggcg catagccagt ggcgaaactc
     1981 ttccgggaaa acatcgcccg gcgaaacgct gtaccacggt tcgctggcaa gttcgtcttc
     2041 cggatagcgc ggcggcggga tgtcgcggaa attcacttcc gtcatgtagc aaatttcatc
     2101 gtaatcataa aaaaccacac gcccgtgacg ggtgacaccg aagtttttaa acagcatgtc
     2161 gccagggaaa atgttagcag cggcaagctg gcgaatagcg ttaccgtatt cttcaatggc
     2221 gtcgcgcaac tgctgacctt ccacttgttc cagccagata ttgagcggca ccatccgccg
     2281 ctcaatataa agatggcgaa tcacaatttg ttcgccgaga tcggtgattt tttccgctgc
     2341 ttcctggagc agtaattcca ttaatgccgg ggaaatatgc cgcttctcca gcacaaagtt
     2401 ttcaaactcc tgggtgtccg ccattcggcc cacacggtcg tgctctttca ccagttgata
     2461 acaggcacga acgtgagcgg cagacatctc tttctgcggc gcgaacttgt ctttgatcac
     2521 tttaaatacc cggtcaaagc ccggcagcgt gaacaccaac atcaccatgc cacgaatacc
     2581 cggcgcttca atgaactgct cattacagcc ctgtagataa acgagatatt cgcggtagct
     2641 ttcggttttg gcgtgcttct ggcagccgat agccatatac aattcagcgg tggttttacc
     2701 tggcagaatt tcccgtagcc actcgaccag cgctgcgggc agcggcgcat aaaccataaa
     2761 ataagaacgc gcaaagccaa aaacaatgct cgcttcggcg gtcgtcgtca ggcaggtatc
     2821 aataaataac tcgccgtcgt ccgtctggtg gatcggcagc aaaaatggca atgtgccgga
     2881 aggtgtgatc agtttgccta ccagccaggc ggctttattg cggtaaaaca gttcgttcgc
     2941 cacctgtaaa tgactttccg cgaggttgtc tgtccccagc gtttccgtca gatggcgaat
     3001 gatgtaatga atgtcacggc ttttattctg ccagcgcagg cgcagcggta ggtcgctgat
     3061 aacgcgcatc agtagagatt cccagccgtg atcggggtga aagtctttcg ccagcgggcg
     3121 gggaatggta cgaaagcggc gctctggctg agagctaaag ataaaaagcc gctcgggagt
     3181 aagcgagcgg tggtcaaata accgacagta cacggagtta aaaaagctct ccgcaatctc
     3241 gaagcgcggg taatccggca acagccgggt gtaatgctct ttaacacgta gtaaaaatgc
     3301 cgcgtccgta ctttggccgt tagtaatgca gcgcagttgc tccacgacca gaccaacgtg
     3361 atgatcgtaa agatggatac ggtttttcat cgcctgctgg acagcatgcc agtcggcctg
     3421 ttcgaaacgc tgctgcgccc cggaagtcac ttcgaggaat cgaccatact gagcgtcgaa
     3481 gccttgcaaa atggtttgag caatcagtaa ttccaggcca cgcgacattt agcgccctca
     3541 tcaggagcag agaattgccg gatgcggcgt aaacgcctta tccagcctac gttcggcaac
     3601 ggttgtaggc atgataagac gcgcaagcgt cgcatcaggc atcgtgcacc gattgccgga
     3661 tgcggcgtaa atgccttatc cggcctacgt tcggcaacgg ctgtaggcat gataagacgc
     3721 gcaagcgtcg catcaggcat cgtgcaccga ttgccggatg cggcgtgaac gccttatccg
     3781 gcctacagtc agcaacggtt gttgttgctt agaactgcga ttcttcagtg gagccggtca
     3841 gcgcggtgac tgaagacgtg ccgccctgaa taatagtcgt cactttatcg aagtaacctg
     3901 tacccacttc ctgctggtga gatacgaagg tatagccatc tttcgcggcg gcaaattccg
     3961 gctgctgcac tttctcgacg tagtgcttca taccctcgcc ctgggcatag gcgtttgcca
     4021 ggtcaaacat gttgaaccac atgctgtgga tacctgctag ggtgatgaac tggaacttgt
     4081 agcccatatc cgacagctgc tgctggaagc tggcaatagt tttgtcgtcg aggtttttct
     4141 gccagttgaa cgacggcgag cagttataag ccagcagttt gcccggatat ttcgcgtgga
     4201 tagcttgtgc aaagcgacgc gccagttcca gatccggcgt ggaggtttca caccagacca
     4261 ggtcagcata tggcgcatac gccaggccac ggctgatcgc ttgctcaatg cccgcatgag
     4321 tacggaagaa gccttcactg gtacgctcgc cagtaataaa ttcactgtca tacgggtcgc
     4381 aatcggaggt aatcagatcc gccgcatcgg catcggtacg agcaaccagc agggttggaa
     4441 cgcccgtcac gtcagctgca agacgcgccg cgaccagttt ctgaatagct tcctgagttg
     4501 gcaccaaaac tttgccgccc atgtgaccgc atttcttcac tgacgccagc tgatcttcga
     4561 agtgaactgc cgctgcaccg gcttcaatca tcgctttcat cagttcaaag gcattcagga
     4621 caccgccaaa accggcttcc gcatcggcaa cgatcggcag gaagtaatcg acatagcgcg
     4681 gatcgcccgg ctcaatgcct gcggaccatt ggatctgatc ggcacgacgg aaggtgttgt
     4741 tgatccgctc caccacagct ggcaccgagt ttgccggata gagcgactga tccggataca
     4801 tgctggccgc caggttagcg tccgccgcta cctgccatcc cgacagatag actgcttcaa
     4861 tacccgcttt cgcctgttgc agcgcctgac cgccagtcag tgcaccgagg ctgttgatgt
     4921 agcctttttt cgactcgcca tgcagcagac gccacatttt cgccgcgccc agttgcgcca
     4981 gggtgcattc aggattgact gaaccgcgta atttcaccac atcttccgcg ctgtatgggc
     5041 gagtaatgcc ttcccaacgc ggttgagtcc actctttctg taattcttca atttgttgtg
     5101 tacgggtttt catgtgcaga tgctccatag ttatgtggtg gtttacgcta acaggcggta
     5161 gcctggcagg gtcaggaaat caattaactc atcggaagtg gtgatctgtt ccatcaagcg
     5221 tgcggcatcg tcaaaacgcc cctgggagaa acgttcttcg cccagttcgc tggcaatgac
     5281 tttcatctct tcacccagca tctggcggaa caaggctttg gtcaccggtt tgccattgct
     5341 caacgttttt tgatgatgga tccactgcca gattgaggta cgggaaattt cagccgtcgc
     5401 cgcatcttcc atcaggccat aaatcggcac gcagccgttg ccggagatcc atgcttcgat
     5461 gtactgcact gccacgcgaa tgttggcgcg cataccttct tcggtgcgtt caccatcaca
     5521 aggtgccagc agctgatcgg cagtaatcgg cgcgtcttgt tcgcgcatca cttcaagctg
     5581 atttttacgg gagccgagaa tgtcgttgaa taccgccatt gccgtatccg caaggcctgg
     5641 gtgagcaatc catgtgccat cgtgaccgtt attggcttcc agcgctttat ccgcttttac
     5701 tttgttgagc acctggttat tgcgctcttc atctttgctc ggaataaacg ctgccatgcc
     5761 gcccatcgca aaagcaccgc gtttatggca ggttttaatc aacaggcgtg agtaagcatt
     5821 caggaatggt ttatccatcg tcactgcctg tctgtctggc aggacgcgat cgggatagtt
     5881 tttcaacgtt ttgatatagc tgaagatgta atcccaacga ccgcagttca gaccaacaat
     5941 atggtcacgc agcgcgtgaa ggatttcatc catctggaac acggcgggca gcgtttcaat
     6001 cagcaacgtc gccttgatgg tgccgcgcgg cagattaaag cgatcttctg catagctgaa
     6061 gacttcgctc caccaggccg cttcctgcca ggactgggtt ttcggcagat agaaataggg
     6121 accgctgccc tttgccaaca gtgcctgata gttgtggaag aaatagagcg caaaatcaaa
     6181 caggctaccg gggattgcct caccacgcca ggtgacatgt ttttccggca agtgcagacc
     6241 gcgtacccga caaatcaaaa ccgctggatt gggcttgagc tgataaattt tgcctgcttc
     6301 attggtatag ctgatggtgc cgttaaccgc atcgcgcagg ttaatttgcc cgtcgatcac
     6361 tttgttccag tctggtgcca gtgaatcttc gaaatcggcc ataaagactt tcacattggc
     6421 gttcagtgcg ttgatcacca tcttgcgctc aaccgggcca gttatctcga ctcgacgatc
     6481 ttgtaagtcc gcgggaatac cacgaatttt ccagtccgta tcgcgaatgg aagctgtttc
     6541 cgaaataaaa tcaggcaaag ttccgttatc aatatcttgc tgctgctgaa tgcgcgctgc
     6601 cagaagttta ttgcgttgtg gcgtaaaatg cgtcaccagc tcagtcagaa attctaccgc
     6661 ttcggcagta agaatttgct tctcatgctc gccatacggc cttatgaaag ccagttcatc
     6721 ggttgttgtt gcctgttcag tcatcgtgca gctcctcgtc atggatccga aaacttcccc
     6781 actgtgaacg aaggatcgtt gtgcgctttt cgttcagcac aactaagact actcatttaa
     6841 aatgcaaaat caaaaacaat ttccatttta attaaaaata cttgataact tattgataac
     6901 aatatgaata aaatttattc acctcctgag gtgcgtttcg gaaataaaaa aggcacccga
     6961 aggtgcctga ggtaaggtgc tgaatcgctt aacgatcgac tatcacagaa gattaatcca
     7021 gcgttggatt catgtgccgt agatcgtatg gcgtgatctg atagacgtaa tagttgagcc
     7081 agttggtaaa cagcaaatta ccgtgactac gccagctcgc tcgcggtgta ttttgcggat
     7141 cattgtgcgg gaaatagtta tacggtacat ccgggtctag tccggcttcc acatcgcgga
     7201 aaaattcctg cgccagcgtt tgcgcatcat attcgggatg gcccgtcaca aaggcaatgc
     7261 gcttatcttt gctggcaaac agatatgcat ccccttcttc cgtctctgcc agaatttcca
     7321 gatcggtgta atcacgaatc aacgctgccg gaaagtcagc atagcgcgaa tgtggtgcca
     7381 ggaatgaatc atcaaagcca cgcgtcagaa gcgcatgagg atggagaata tgatgctcgt
     7441 aaacgccaga gagtttgtcg gtgcgagttt gcttaggaat gccgtagagg atattgagcg
     7501 cggcctgtac cgcccagcag acaaacagcg tcgaggtgac gtgatctttc gaccactcca
     7561 gcacctgttt gatctgcggc cagtaagcga catcattaaa ctccaccagg cccagcggcg
     7621 cgccggtaac tatcagaccg tcaaagtttt gttcctgaat atcttcaaag ttacagtaga
     7681 agttgttcag atgctctgcg ggcgtgttgc gcgattcacg ggaatcgatg cgcaacagct
     7741 gaatatcgac ctgtaagggc gagtttgaaa gcaggcgcag aaactgattt tcagtttcga
     7801 tcttcttcgg catcaggtta aggataagca cctttagcgg acgaatttcc tgaccagacg
     7861 cgcgagaagt tgtcatcaca aagacatttt cttcacgcaa gaaattgacg gcgggtagct
     7921 cgtccggcac acgaatcggc ataacctgat tacctcacta catacgctta tacgtttaga
     7981 catccagata gctgaagata accagaaaat ttgccaatgt cgagcctgca tgttgaaggt
     8041 gagaaagttt caaggaaaag gctacgttag aatataagaa tgacgaaaag gagagaaaat
     8101 ggttattagt attcgccgct cacagcatga ggaaggggag gaactcgttg cgatttggtg
     8161 tcgatctgtc gatgccaccc acgattttta tcagcagagt atcgggccga gctggaagag
     8221 ctggttcgtt cctttctgcc ggaagcaccg ttgtgggtcg cggttaatga acgggatcag
     8281 ccggttgggt ttatgttgtt aagtgggcag catatggatg cgctgtttat cgatcctgat
     8341 gtgcgcggct gcggcgtggg tcggatgctg gtggagcatg cgctctcgat ggcaccggaa
     8401 ctgacaacca acgttaatga gcaaaatgag caggcggttg ggttctataa gaaggtgggt
     8461 tttaaggtta cgggacgctc tgaggtggac gatttgggga aaccgtatcc gttgctgaat
     8521 ctggcgtatg tgggggaata agccgcccct gccgtgcctg cccaggctgg agaagacgcg
     8581 ccagcgtcgc acccggcatt tttttcacca acgcaaaaag gccatcctga cggatggcct
     8641 ttcgc
//
LOCUS       DAZKIV010000169         8590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZKIV010000169 DAZKIV010000000
VERSION     DAZKIV010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.169"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            454..1695
                     /locus_tag="ACHOT6_004058"
     CDS             454..1695
                     /locus_tag="ACHOT6_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFP9949197.1"
                     /translation="MNLQSGQNIPLQQSTIRLNLQYPAKSGFKGEPDTCLFMLNAQGK
                     VSGDSDFIFYNNLSSPEGAVRLVTGSQQASIEIALDRVPANVSKIAITVVIDGEDTIS
                     GLSLLSIQAPGIADFQAETQGRSEKAIILGEVYRHNGAWKFRALGQGFNGGLEPLAIN
                     YGVDVAQPAPQPAKPARISLEKKLETRSPRLVSLAKKASVSLTKNKLDTLEAAVAFVL
                     DASGSMSGQFSKGNVQSVLDRIAVLAAQFDDDGEMDVWGFGEKHKKYPNVTLDNLDTY
                     IQSIRGAGKRSSWENLPGLGGTNNEPPVMEEIVDYFKDSKIPVYVVFITDGGISKTRA
                     IKDAIRRSANYPIFWKFVGLGGSSYGILKNLDDFTDRRVDNTHFFAMDDFGSISDEKL
                     YDNLLEEFRPWIDETKRLGIL"
     gene            1853..2278
                     /locus_tag="ACHOT6_004059"
                     /pseudo
     CDS             1853..2278
                     /locus_tag="ACHOT6_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2275..2349
                     /locus_tag="ACHOT6_004060"
                     /pseudo
     CDS             2275..2349
                     /locus_tag="ACHOT6_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2562..2744)
                     /locus_tag="ACHOT6_004061"
     CDS             complement(2562..2744)
                     /locus_tag="ACHOT6_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949198.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNSEDN
                     DEAASDYALKINESSD"
     gene            3075..3947
                     /locus_tag="ACHOT6_004062"
     CDS             3075..3947
                     /locus_tag="ACHOT6_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFP9949199.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILLCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTDAVFRLFRPVHPVVAVSARTGWELDTLVRALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            4165..6561
                     /locus_tag="ACHOT6_004063"
     CDS             4165..6561
                     /locus_tag="ACHOT6_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFP9949200.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENRLL
                     FDKQKALQRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLSTLQQKLDTPAGQQVAQTLQQTGDTCELL
                     DILANDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGSEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFVREAFGRMKKEYVCKDSEMATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQVTADVRMAMEEIDARTRELLTGVCTSLEEELNHYFRTG
                     KRKEQQMLEEEDAEQRRSRSGLWGMISQWSGINNPGWEDYRKRDFDPKNPEITLNNRR
                     EAIEYIEEIESTVTTLHREAEAQFRPELEKIVRGIETGFRGTALYATENIAGRINARL
                     EDEGFTVKISFPAVSQLQTRLAVKTNLSALMEERTETITRRRRKDSFIGKICGWIGTK
                     EWGWETYKEDVSRSVININKVREEVMSLTRAYFGELQASIEQDINQPVRQEIDAFFCT
                     FREKVEQLRNTLIQSSEDHKRDQQAQERLTGRLQALNERVPELITDSKALREELETML
                     "
     gene            6558..7463
                     /locus_tag="ACHOT6_004064"
     CDS             6558..7463
                     /locus_tag="ACHOT6_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFP9949201.1"
                     /translation="MTSPFIQQIADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAG
                     ERTFFRRLKEGLTGEGAARQNAINASLAQGVEASLRWLTELTTSLASTNYAITRVNDR
                     VSSLVSDTARLAHYSADTREQLLTLADHVHQKLNHLEEKLHRVDQVQRAQLHLEQIFS
                     WWSAGRYASFSPAGRCYVALEELRWGAFGDVIRQGETGQVNQLLDILRHKALTQMAQE
                     SGGSATVRLNTLDWLGGQGREQADNEWHDAINWLGDWCSEEQHPVIWSTTQAAEHLPV
                     RMPRLCSAERLSESMVDEIFQKGAA"
     gene            7460..8530
                     /locus_tag="ACHOT6_004065"
     CDS             7460..8530
                     /locus_tag="ACHOT6_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFP9949202.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGTQISMVSGASIG
                     AFNGAIIAASPDLSEAAVRLEALWDHLGNNQVLSVNRSVYFSLLKKLLQAMNLCQIPG
                     RAGALLTTLLRHISIINGFDNPMTQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYP
                     TEGGMQDIIDCIRAELGVGTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQG
                     TMFGDGGMGGWRNMQGNTPVTPLVDAGCNMVIVSHLSDGSLWDRRAFPDTTILEIRPR
                     KRLKHTGDGGNSGGLLSFASAHTDAWRQQGYEDTMLTMEHIRKPLAARQALSRSEAVL
                     QKSLDITEEADLALRNAMARIK"
BASE COUNT         2235 a         2056 c         2279 g         2020 t
ORIGIN      
        1 gcgccccacc agacccggtg acatcacccc ccggatcatg gacgactggg ggcagtaatc
       61 atgccgggtc tggctggcta acaccccatt ataaggggta cgaataaagt aacgggggca
      121 gaaagcagag ctatgttaat tctggcatga accttttatg caggccccgg gtttatctgt
      181 ggttgctggt taaagccagt attccgtgtc tgatatcaac agcatgccct tcggatatat
      241 gttacttttg cgacttatga aggaacgggc cctggaaaat aagatacccg gtcaatgaaa
      301 attcaaattc aacaggtata gaaacttcag cgttatgaaa aatggtgtca taccatattt
      361 acgcctcacc gaatatccag cgtccagaac taagcctagg gaacatatat aaacgctgcg
      421 cattaataat aatcaaaaat caggagagtt aatatgaatt tacaatccgg acaaaacata
      481 ccccttcagc aatctacgat caggctgaat cttcagtacc ctgccaaatc cggctttaaa
      541 ggcgaacccg atacctgcct gttcatgctt aatgctcagg gaaaggtcag tggcgattct
      601 gactttatct tttataataa tttgtcttct cctgaggggg cagtcaggct cgttaccggt
      661 tcgcagcagg ccagcattga gatagcactg gatcgtgttc cggcgaacgt cagtaaaatt
      721 gcgatcaccg ttgtcattga tggtgaagat accatcagtg ggctcagttt attgagcatt
      781 caggctcctg gtatcgctga tttccaggct gagactcagg gccgcagtga gaaagcaatc
      841 attctgggtg aagtatatcg gcacaatggc gcctggaagt ttcgtgcgct agggcagggt
      901 ttcaacggtg gacttgagcc tttggccatt aactatggcg ttgatgtcgc acagccagct
      961 ccgcagccag caaagcctgc tcgtatcagt ctggaaaaga aactggaaac ccgatctccg
     1021 cgccttgtaa gcctcgctaa aaaagcctcg gtcagtctca ctaaaaataa actggacacc
     1081 cttgaggctg cggttgcgtt cgtacttgac gcatccggct cgatgagtgg ccagttcagt
     1141 aagggtaacg ttcagtctgt gctggaccgc atcgccgttc ttgccgccca gttcgacgac
     1201 gacggtgaaa tggatgtctg gggatttgga gagaagcata agaagtatcc aaacgtcact
     1261 ctggacaatc tggacacgta tattcagtcg attcgagggg ctggtaagcg ttcatcctgg
     1321 gagaacctgc cgggccttgg ggggacaaac aacgagcctc ctgtgatgga agaaatagtc
     1381 gactacttta aggactcgaa aatcccggta tatgttgtat ttattaccga tggcgggatc
     1441 agcaagaccc gggcgattaa agatgcaatc cggcgttctg ccaactaccc catcttctgg
     1501 aagtttgtcg gcctgggtgg ttcaagctac ggtatcctca aaaatctgga tgactttact
     1561 gaccgccggg ttgataacac ccacttcttt gccatggatg atttcggttc gattagcgat
     1621 gaaaagttat atgataattt actggaagaa ttcagaccgt ggatcgatga aacaaaaagg
     1681 ttaggcatcc tttaatacat atagttagag catgggtgaa tgagctctac agcagcttaa
     1741 tactgtagag cctttcttca ttaaaaggta aacgtaaacc gaccgccata tgtagccatt
     1801 agacaagaat tggtaactta gacgcccatc tgacacagac ggacatctaa gtatggaatt
     1861 acaggactgg cgaaaagaac ctcgtaaaaa gtattcgaat gaattcaaac ttcgtatggt
     1921 tgaactggca tcacaacccg gtgcttgtgt tgcacagatt gcacgtgaaa atggcgtcaa
     1981 tgataatgtt attttcaaat ggctcaggct ctggcagaac gaagggcgtg tttcgcggtg
     2041 tcttccaata acgacctctt ctgacactgg cgttgaatta ttacctgtag aaataacgtc
     2101 ggatgagcag aaagaacctg tggcgtccat tacgccgtct ttacccgctt ccacttagac
     2161 cagagtcagt gccagttcct gcaaggtgga attccgtcac ggtaacatga tgctggaaaa
     2221 tccatcgcca gagctgctca cggtgttgat ccgcgaactg agcgggaggg gaagatgatt
     2281 tcactcccat caggtacccg tatctggctc gttgccggtg ttaccgatat gtgtaaatct
     2341 ttcaacggat acgacatatg ctgaaggagg atctgtaatg gattaagtga taacagatac
     2401 tcagaaatat aggggcaaat ccactatttt gatgagaatt attatatgtt acctcggatt
     2461 ttaatcaggc gccaagatta aataaaatat tgagcgcctg attatattta ttcattcatt
     2521 aatatctgtt attgattggc ttactatcag ctccccccgt attaatcaga gctttcattt
     2581 atttttagtg cataatcaga tgccgcctca tcattatcct cactattatc atagccgaaa
     2641 acttcccccg gacacgggcg ttcacgataa tagtcatcgt catagttttc atcgtcagat
     2701 aatgatatgt cgtattctga atatgattca ccacaattat acatgaaatc atctcctttg
     2761 gggtggttac aataattagt gagtttattc taaaaaatga tgagcgccag accaacccac
     2821 ccaacttttg attataatca tgaagatact tgtctcagta caaatagaat caaactcagt
     2881 gcagcatgat tcacctttat attctctcag ggaaaccaaa taccactact caactattaa
     2941 gaacaaaatt ctggaggtat gattatctga agatacctgc atcctaaata atttcagaca
     3001 gatattatcg ttgtgagtcc cccgccacct ttccggtgtg cggggttttg tcttttttca
     3061 ccgggaatac atgtatgaat ccgtctgatg ccattgaggc aattgaaaaa ccgctctcct
     3121 ccctgcctta ctcactttcc cgtcacatcc tggaacatct gcgtaaactc accagtcacg
     3181 aacccgtgat tggcattatg ggtaaaagcg gggccggtaa atcctcactc tgtaatgccc
     3241 tgtttcaggg ggaggtcacc ccggtcagtg atgttcacgc cggcacccgg gaagtgcagc
     3301 gcttccgtct gagtggccat ggtcacagca tggttatcac tgacctgccc ggggtgggcg
     3361 agagccggga cagggatgca gagtatgaag ccctgtaccg tgacattctg cctgaactgg
     3421 acctggtact gtggctgatt aaagccgatg accgtgccct gtctgtggat gagtatttct
     3481 ggcgacacat cctgctatgc ggacatcagc aggtgctgtt tgtggtgacg caggccgaca
     3541 aaacggagcc ctgccatgaa tgggatatgg ccggtattca gccttctccc gcacaggaac
     3601 agaacattcg tgagaaaaca gatgcggtat tccggctgtt ccggcccgta catccggttg
     3661 tggccgtatc ggcccgcacc ggctgggaac tggatacgct ggtcagagcg ctcatgacag
     3721 cgcttcccga ccatgccgcc agtcccctga tgacccgact gcaggacgag ctgcgcacgg
     3781 aatctgtccg ctctcaggcc cgtgaacagt ttaccggtgc ggtggaccgg atatttgaca
     3841 cagcggagag cgtctgtgtt gcctctgttg tccgtacggc cctgcgcgct gttcgtgaca
     3901 ccgtggtctc tgttgcccgc gcggtatgga actggatttt cttctgaacc cggtggtgat
     3961 gcaccagact cttgttacag atttttaacc ccatgaaaac acaatgcaaa cacagccata
     4021 cccgggttgt ccccttgcgt tgtgtttctc aagttaatag aatgcatcga tcgttactac
     4081 catatcgatc gttataaacg atcgtttgtg gtaaatgacc tcctccgtgg ttgtcatgaa
     4141 aatgcgtctc aggaaggtta aataatgcac gaaaagaaca tcgccctgct ttgtgatgaa
     4201 gccgaccgac ttttgcaact gaacattaat ctgctccggc aaatggttga tgaaccggat
     4261 gtgttatctg acagtaagaa cgaaaacaga ctgctttttg ataaacagaa agcactgcaa
     4321 agaattgagg agctggaggg cgaacaaatc aaaaccgccc gcagggagat ggtgctggct
     4381 gttgtcggca cgatgaaagc aggtaaatca actaccatca acgccattgt ggggcaggaa
     4441 attctgccta accgtaaccg ccccatgacc tctgtaccga cgctcatccg ccacgttccc
     4501 ggaaaaactg agccggtcct ccatctggaa catattcagc ccgtccgcaa tttattaagc
     4561 acactgcaac aaaaactcga caccccggca ggacagcaag tcgcacagac cctgcagcaa
     4621 accggggata cctgcgaact gctggatatt ctggcaaatg atggctggct caaaaatgaa
     4681 taccacgggg aggaggaaat ctttaccgga ctggcatcgt taaacgatct ggttcgcctt
     4741 gctgcggcaa tggggagtga gtttcctttt gatgaatacg cagaagtgca gaaactgccg
     4801 gtgatcgacg tggaattcag ccatctggtg gggatggatg catgccaggg aacactcaca
     4861 ctgctggata cccccggccc taatgaggcc ggacaaccgc agatggaagt gatgatgcgg
     4921 gatcaactgc agaaagcctc tgcggtgctg gcggtgatgg attacaccca gatgaactca
     4981 aaagcggatg aagacgtccg taaagagctt aatgccattg ctgacgtatc agccggccgc
     5041 ctgtttgtgc tggtcaataa atttgatgag aaagaccgca atggcgatgg ggcagatgcc
     5101 gtacgccaga aagttccggc aatgctgaac agtgatgtgc tgcccgcctc ccgcgtttat
     5161 ccaggctcat cacgtcaggc atacctggct aaccgtgcgc ttcatgagtt acggaaaaac
     5221 ggaacccttc ctgttgatga agcctgggtc gatgattttg tcagggaagc cttcggtcgc
     5281 atgaaaaaag aatacgtctg taaagacagt gaaatggcaa ccgaaggggc gacagacctg
     5341 tgggaaggtt cacttatcga tcaactgata acggaagtca tacagagctc ccattccaga
     5401 gcggcggcac tggcggttga ttctgccgcc gcaaaactga tgcagaatgc ggaaaatgtc
     5461 agcgaatatc tgtcattacg ccatcagggg ctacagcaaa gcattcagtc actgcagtcg
     5521 catatcacca gcctgcttgc ggatatccgg gaaatcgcgg actgtcagga gcaggtgacc
     5581 gctgatgtca gaatggccat ggaggagatc gatgccagaa cccgggaatt actcacgggg
     5641 gtctgcacct cactggaaga ggagctgaat cactatttca gaaccggtaa acgcaaagaa
     5701 cagcaaatgc tggaggaaga agacgcagaa caacgaaggt cccggtccgg attatgggga
     5761 atgatttctc aatggtccgg tatcaacaac ccgggctggg aagattacag gaaacgagac
     5821 tttgacccga aaaaccccga gataacacta aataatcgca gggaagcaat tgaatatata
     5881 gaggaaattg agtcaactgt gaccaccctg caccgtgagg ctgaagcaca gttccggcct
     5941 gagctggaga aaatcgtcag gggtattgaa acaggttttc gtggcacggc cctgtatgcc
     6001 actgaaaaca ttgccggtcg catcaatgcc cgcctggagg atgagggctt taccgtaaaa
     6061 atcagttttc cggcagtcag ccagttacag acccggctcg cagtaaaaac aaatctgagt
     6121 gcgcttatgg aggaaagaac ggagacaatc acccgtcgcc gtcggaagga tagcttcatt
     6181 ggaaagattt gtggatggat tggcaccaaa gaatggggct gggaaaccta taaggaggat
     6241 gtgagccgca gcgtgatcaa tatcaacaag gtcagagagg aagtgatgtc actgacccgg
     6301 gcatatttcg gggagctgca ggcatccatt gagcaggata ttaaccagcc cgtccgccag
     6361 gaaatcgatg cctttttctg tacattcagg gagaaagttg aacagctgcg taacacgctg
     6421 attcagagct ctgaagatca taaacgcgat cagcaggcgc aggaacggct taccgggcga
     6481 cttcaggcat taaatgaaag ggttcctgag ctcattactg acagtaaggc gctgagggaa
     6541 gagctggaga caatgctgtg acttcaccat ttattcaaca aattgctgat aaccgggtat
     6601 gtcaggtact cacctgtctt cctgaaaaat ttgtggttga tttcgcaaac ggtatcgatg
     6661 ttgcacagga gcacatccgc acggccggag agcgcacgtt cttccggcgt ttaaaagagg
     6721 gactgactgg cgaaggcgct gcacggcaga atgccatcaa tgcttcgctt gcacagggcg
     6781 ttgaggcgtc cctgcgctgg ctgacggagc tgacaacgtc gcttgcctcc acaaactacg
     6841 cgattacccg ggtaaacgac agggtcagtt cactggtcag tgatactgcc aggctggcgc
     6901 attattccgc agatacgcgg gagcagttac tcaccctggc cgatcatgtt caccagaaac
     6961 tgaatcatct tgaagaaaaa ctccaccgtg ttgaccaggt tcagcgggcg cagttacatc
     7021 ttgagcagat attctcatgg tggagcgccg ggcgatatgc gtcattttcc cctgccggac
     7081 gttgttatgt ggcacttgaa gagcttcgct ggggcgcgtt tggtgatgtg attcgtcagg
     7141 gcgaaacagg ccaggttaac cagctactgg atattctcag acataaagca ttaacgcaga
     7201 tggcacagga gagtggcggt agtgcaaccg tgcgtctgaa cacgctggac tggcttggtg
     7261 gtcagggccg ggagcaggca gataacgagt ggcatgacgc gattaactgg ctgggggact
     7321 ggtgcagtga agagcagcat ccggtgatct ggtcaaccac gcaggctgca gaacacttac
     7381 cggttcgcat gccccgtctc tgctctgcag agcgcctctc tgaaagtatg gtcgatgaaa
     7441 tatttcagaa gggggctgca tgagtacaga aatgaaaacg gggctggtgc tgtccggcgg
     7501 cggggcggtg ggtgcttatc aggcgggagt ggttaaggca ctggcagagt gtggtacaca
     7561 gatcagcatg gtttcagggg ccagcattgg cgcattcaat ggtgccatta tcgcggcctc
     7621 tcccgatctg tcagaagctg ccgtacgcct ggaggcgctc tgggatcatc ttgggaataa
     7681 tcaggtgctg tcggtaaaca gatcggttta cttttcattg ctgaaaaaat tgctccaggc
     7741 aatgaacctc tgccagatcc ccggacgtgc aggagcactg cttacgacgc ttcttcgcca
     7801 tatatcgata atcaacggct tcgacaatcc gatgactcag ccgttgttgt cagatgagcc
     7861 cctgacagcg ctgatggatc attatcttga tactgatgct ctggcagacg ggctaccgct
     7921 gtatgtgtcg ctgtacccca cagaaggggg catgcaggat attattgact gcattcgtgc
     7981 tgaactgggt gtcggaacca cgaaaaacgc cgtttttcag catatccaga gtctgccccg
     8041 cggacagcag aaagaggctc tgcttgcgtc agccgcgctg cccctgttgt tccgtccccg
     8101 tgaggttcag gggacaatgt tcggtgatgg tggtatggga ggatggcgaa atatgcaggg
     8161 aaatacccct gtaacgcctc tggtcgatgc cggatgcaat atggtgattg tgtcgcatct
     8221 gagtgacggt tctttatggg atcgccgggc ttttccggac accacaatcc ttgagatccg
     8281 tccccggaaa aggctgaaac atacaggtga tggtggcaac agcggcggtc tgctcagttt
     8341 cgcgtcggca cataccgacg cctggcgtca gcagggctac gaggacacga tgctgacgat
     8401 ggagcatatc cggaaaccgc tggcagcacg tcaggcactg tcccggtcag aggcggtatt
     8461 gcagaaaagc ctggatataa cggaagaggc agatttggca ctaagaaacg cgatggcccg
     8521 gattaaataa agacgcccca aagaaacgcc accgcctgtg gtggtgtttt tcctgtctgg
     8581 tgttattgaa
//
LOCUS       DAZKIV010000170         8489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZKIV010000170 DAZKIV010000000
VERSION     DAZKIV010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.170"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..206
                     /locus_tag="ACHOT6_004066"
     CDS             <1..206
                     /locus_tag="ACHOT6_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase M85"
                     /protein_id="HFP9949203.1"
                     /translation="LGTISDRYEASPDFTEYSAVSNIGYGFIQQHDFPGVAIDENLQD
                     VNQIQLYHGAPYIFTFGDVDRHN"
     gene            complement(327..410)
                     /locus_tag="ACHOT6_004067"
                     /pseudo
     CDS             complement(327..410)
                     /locus_tag="ACHOT6_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            495..566
                     /locus_tag="ACHOT6_004068"
                     /pseudo
     CDS             495..566
                     /locus_tag="ACHOT6_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsid protein"
     gene            complement(577..819)
                     /locus_tag="ACHOT6_004069"
     CDS             complement(577..819)
                     /locus_tag="ACHOT6_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFP9949204.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDVEVIIKSTS
                     NDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVR"
     gene            951..2234
                     /gene="ydfJ"
                     /locus_tag="ACHOT6_004070"
                     /pseudo
     CDS             951..2234
                     /gene="ydfJ"
                     /locus_tag="ACHOT6_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            2323..3775
                     /gene="ydfI"
                     /locus_tag="ACHOT6_004071"
                     /pseudo
     CDS             2323..3775
                     /gene="ydfI"
                     /locus_tag="ACHOT6_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527761.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(3810..4013)
                     /gene="ydfZ"
                     /locus_tag="ACHOT6_004072"
     CDS             complement(3810..4013)
                     /gene="ydfZ"
                     /locus_tag="ACHOT6_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFP9949205.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4191..4877)
                     /gene="rspR"
                     /locus_tag="ACHOT6_004073"
                     /gene_synonym="ydfH"
     CDS             complement(4191..4877)
                     /gene="rspR"
                     /locus_tag="ACHOT6_004073"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFP9949206.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4966..5712)
                     /gene="ydfG"
                     /locus_tag="ACHOT6_004074"
     CDS             complement(4966..5712)
                     /gene="ydfG"
                     /locus_tag="ACHOT6_004074"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFP9949207.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5849..7894
                     /gene="dcp"
                     /locus_tag="ACHOT6_004075"
     CDS             5849..7894
                     /gene="dcp"
                     /locus_tag="ACHOT6_004075"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFP9949208.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7939..8457)
                     /gene="ydeJ"
                     /locus_tag="ACHOT6_004076"
     CDS             complement(7939..8457)
                     /gene="ydeJ"
                     /locus_tag="ACHOT6_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFP9949209.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
BASE COUNT         2132 a         1947 c         2168 g         2242 t
ORIGIN      
        1 gactgggtac gatcagtgac cgatatgagg cgagtcctga tttcacagag tattccgctg
       61 tgtctaacat aggatacgga tttatccagc aacatgattt tccaggggtg gctatcgacg
      121 agaatttaca ggatgtaaat cagatccaac tgtatcatgg tgctccttat atctttacat
      181 ttggggatgt ggacagacac aattagcgct gattcgtctt tacagttaca tagggttgct
      241 actcatagat attaacggag ttgatgatgg gcaatcgtgc aacattgtat tgtatgtaaa
      301 ggcagagccc cgaattttac tgttgtaagt gggcctggcg ggaatggtat cgccagcatc
      361 attggcttgg gtccagtaaa aagagacgca gaaagatgca caataggcac tacgcgtcat
      421 gcatggatta ggtcgctcat catttacaca gtactgctaa aaaattatta aggaaagcgc
      481 ggcacttcgt atgtaagagc gtgtcgcggt tggctggtga actttcgata gtgcgagtat
      541 tgaatgattt ccagccgtta ccgatttacg tgtttattag cgaacaaacc actcgtcagc
      601 agactcccag gtatctttca gcgtctcctg aacaaaagtt tttgcagaat ctttatcggc
      661 ggtgcgagta actgaaaggc catcgttgct ggtggatttg atgatcactt ctacatcgtc
      721 ataacgttta ctgacacgtc gcagcatttc ttgttgcagg gcaggaacag aaccttttgg
      781 cattttgttg attttggctg cggggtatct gtggggcata tatgggacat aaaaaggcct
      841 caaataaaca gcaaaatcga gattagggat ttttagaaaa aatacaacca tctgaaaaaa
      901 cttagaaaac acccaaaaac ccacagaata gtaaaaaaca tctatgaatt atggatttcc
      961 agttatattc gctcggcgca gcgttagtgt ttcatgaaat attttttcct gaatcatcaa
     1021 cggcaatggc gttaattctg gcaatgggaa cctatggtgc aggttatgtg gcgcgtattg
     1081 tcggagcatt tattttcggc aaaatgggcg acagaatagg gcgtaaaaaa gtgctcttta
     1141 ttaccatcac catgatgggg atctgtacca ccttaattgg agtgttaccg acctatgcac
     1201 agattggtgt ttttgcaccc atcttgctgg tgacgctgcg tattattcag ggattgggtg
     1261 caggtgcgga aatttccggt gccggtacga tgctggcgga atatgcgcca aaaggtaagc
     1321 gcggaattat ctcctcattt gtggctatgg gaactaactg tggaacctta agcgcaacgg
     1381 caatctgggc ctttatgttc ttcattctca gtaaagagga actgctggcg tggggatggc
     1441 gtataccgtt cctggcgagc gttgtcgtga tggtctttgc tatctggttg cgtatgaatc
     1501 tgaaagaaag cccggtcttt gagaaggtta acgacagcaa ccaaccgaca gcaaaacctg
     1561 cacctgctgg tagcatgttc cagagcaaat ccttctggct ggcaacaggg ctgcgttttg
     1621 gtcaggcggg taactccggt ttaattcaga ctttccttgc aggctattta gtacagacgt
     1681 tattgtttaa caaagcaatt ccaacagatg cattgatgat cagttcgatt ctcggcttta
     1741 tgaccattcc gttccttggt tggttatccg ataaaattgg tcgccggatc ccgtatatta
     1801 ttatgaatac ctctgcgatt gtgctggcgt ggccaatgct ttctatcatc gtagataaaa
     1861 gctatgcccc gagcaccatt atggttgcac tgattgtgat tcataactgt gcggtgctgg
     1921 gattatttgc tctggaaaac attaccatgg cagaaatgtt cggctgtaaa aaccgcttta
     1981 cccggatggc tatttcaaaa gaaattggtg gtcttatcgc ttccggtttt ggtcctatcc
     2041 tggcgggtat tttctgcacc atgacggaat cctggtatcc gatcgccatt atgatcatgg
     2101 catattcagt gattggttta atctctgcgc tgaaaatgcc agaggtgaaa gaccgtgatt
     2161 taagtgcgct ggaagacgct gcggaagatc aaccgcgtgt tgtaagagct gcgcaacctt
     2221 ccagaagtct gtaaatcctc aatcccttct cttaccggag aggggatttt tattcatata
     2281 acaaaacatg tagcttgcca tatttaaatt taaggaattg ttatgggaaa taatttgtta
     2341 tcagcaaaag cgacactccc tgtttatgat cgtaataacc tggccccaag aattgttcat
     2401 ttaggctttg gtgcatttca ccgtgcgcat cagggcgtgt atgccgatat tcttgctaca
     2461 gaacatttca gtgactgggg atattatgag gtcaacttaa tcggcggcga acagcaaatt
     2521 gccgatttac agcagcaaga taatctttat accgttgcgg aaatgtcggc cgatgcgtgg
     2581 acggctcgcg tcgttggcgt cgttaaaaaa gccttgcacg tacagatgga tggcttagaa
     2641 accgtgttgg ctgcgatgtg tgaaccgcaa atcgcgattg tctctctgac aatcaccgaa
     2701 aaagggtatt tccactctcc ggcgaccgga cagttaatgc tcgatcaccc gatggtcgct
     2761 gccgacgtgc aaaatcccca ccagccgaaa acagcaacag gagtgattgt tgaggcgctg
     2821 gctcgccgta aagcggcagg acttcccgca tttaccgtca tgtcatgtga caacatgcca
     2881 gaaaacggtc atgttatgcg tgacgttgtc acttcctacg cgcaagccgt tgatgaaaaa
     2941 ctggcacaaa gataacgtga ctttcccatc aacaatggtg gaccgtattg tgcccgcagt
     3001 gacagaggat acgctggcga aaatcgaaca acttaccggt gtgcgcgatc ctgcgggcgt
     3061 tgcctgtgaa cctttccgcc agtgggtaat agaagataac tttgttgccg gacgtccgga
     3121 atgggaaaaa gcgggagccg aactggttag cgatgtgctg ccttatgaag agatgaagtt
     3181 gcgcatgctc aacggcagtc attcattcct ggcgtatctg gggtatcttg ctggatatca
     3241 gcacattaat gactgcatgg aagatgaaca ttatcgttat gcggcgtatg gcttgatgtt
     3301 gcaggaacaa gcgccgacgc tgaaagtgca gggcgttgat ttgcaagatt acgctaaccg
     3361 attaattgaa cgctatagca acccggcgtt acgccatcga acctggcaga ttgcgatgga
     3421 tggcagccag aaattgccac agcggatgtt ggattctgtt cgctggcatc tggcgcatga
     3481 cagcaagttc gagctgctgg cactgggcgt cgcgggttgg atgcgttatg tcggtggtgt
     3541 tgatgaacag ggaaatccga ttgaaatcag tgacccactg ttacctgtta ttcagaaggc
     3601 tgtacaaagt agtgccgaag ggaaagcgcg cgtccagtca ttgctggcga ttaaggcgat
     3661 ttttggtgat gatttgccag ataatagctt gtttactgca aaagtgacgg aagcgtactt
     3721 gtctttatta gcgcatggtg cgaaagcgac cgtggcgaaa tattccgtga attaaaaaag
     3781 caatgccgcc atcggcggca ttcacacgct tagttcatgc cgagacgaat caggtccaga
     3841 ggtgccagtt tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca
     3901 gcggtcagac cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc
     3961 ataacacggc tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt
     4021 ttctcctttc tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc
     4081 gagtgaaatg ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg
     4141 taaggcgggg ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg
     4201 ctaaaccagt cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa
     4261 tgttgcgtca ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata
     4321 tcaagatgct ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc
     4381 acgcgatcaa cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca
     4441 atctgcgtca ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg
     4501 cgctcaatgg caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg
     4561 gtaatcatgc tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg
     4621 ccgttgcgca cctgggccat ggaaattttg ttgacgtagc tgccacgctg tggacgaatt
     4681 tgaatcaggc cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca
     4741 ttgaaacgaa cagaaacttc tttttccgac aacggtgtgc ctggagcaat caggcaatgg
     4801 acaatgtcgc gacgaaggat acgataaatt tgctgattga caggctgtgt gggattaagt
     4861 tgcgtttcga cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact
     4921 tttttgtgac aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac
     4981 attcagtccg gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg
     5041 agcaggcagc gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac
     5101 ggtattttga taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa
     5161 ctcggtgcca cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag
     5221 atccgtacgc agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt
     5281 accaccggca tacggccagc tacctgccgt tgagccaatg ttaataatat gaccatgatt
     5341 acgttcaacc atacccggta agacggcgcg cgtcatatat accaggcctt tgttgttggt
     5401 atcaatcatc gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc
     5461 caagccggca ttatttacca ggatatcaat attgcaccac tcggcaggaa gcgatgccag
     5521 catctcttca atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc
     5581 cagttcgtct tttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg
     5641 cccttgttga ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac
     5701 taaaacgatc atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg
     5761 cttaagagcg ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta
     5821 tggaacacct cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag
     5881 cacactgccg tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc
     5941 attcgatgag ggaatgcagc aaaagcgggc agaaattgct gccatcgcgc ttaacccgca
     6001 aacgcctgat ttcaacaata ctattctggc actggaacaa agcggagaat tacttacccg
     6061 cgttaccagc gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct
     6121 tgatgaacag ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga
     6181 attattcgcg cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga
     6241 atccatccgc ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc
     6301 gcaagctgat aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca
     6361 gtttaaccag cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc
     6421 gcagctggca ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa
     6481 aggtctggat aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc
     6541 cgaaatgcgc gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga
     6601 aaaaaatgat ggcaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca
     6661 gcaggcgaaa ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc
     6721 aaaaacgcca gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg
     6781 tgcgagcgat gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag
     6841 cgcgcagccg tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct
     6901 tgatgaggcg cagctcaagc catattttga attaaacacg gtgttgaatg aaggtgtatt
     6961 ctggaccgcg aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttcctgtcta
     7021 ccatcctgac gttcgggtgt gggaaatttt tgatcataat ggcgtggggc tggcgttatt
     7081 ttacggtgat ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt
     7141 tgagcaatca acgcttaatg aaacgcatcc ggtaatttat aacgtttgca attatcagaa
     7201 acccgctgcc ggtgagcctg cgttgttact ctgggatgat gtcataacct tattccatga
     7261 atttggtcat acgttgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac
     7321 caacacgccg cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca
     7381 tccgcaggta ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga
     7441 actgcaacag aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact
     7501 gcttagcgcc gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca
     7561 ggatgtcgat gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat
     7621 accgccacgc tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg
     7681 ttattacgct tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga
     7741 gcagggcgga ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg
     7801 taacagcgaa gatctggaac gcctgtatcg acaatggcgc ggtaaggctc ctcagattat
     7861 gccgatgctg caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat
     7921 atcgccaggc cagcctggtt atagcagtaa ttgcagcagc tggacgaggg caaacctcac
     7981 cgctaaagct aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtaggtctg
     8041 gcctttaata tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc
     8101 cggtccgccg tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc
     8161 tgttgccatt tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagattgctg
     8221 gcttacgctg aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta
     8281 aaatttgggt gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga
     8341 ttcggcggta gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag
     8401 gttttcaata gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt
     8461 tttttgtcca cgctattata tgtaaaggt
//
LOCUS       DAZKIV010000171         8482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZKIV010000171 DAZKIV010000000
VERSION     DAZKIV010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.171"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..324)
                     /locus_tag="ACHOT6_004077"
     CDS             complement(<1..324)
                     /locus_tag="ACHOT6_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949210.1"
                     /translation="MFSTNLIQSNYGDLNIKSLAFDSFKERLESTMAALTFFISTGQC
                     DCDEIAESNFNRMIAYMSNINYDASKPGAPALSFDTYLQDNVKYRVIINNLYGSEFRI
                     RDINEN"
     gene            complement(613..876)
                     /gene="cedA"
                     /locus_tag="ACHOT6_004078"
     CDS             complement(613..876)
                     /gene="cedA"
                     /locus_tag="ACHOT6_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFP9949211.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            1059..3320
                     /gene="katE"
                     /locus_tag="ACHOT6_004079"
     CDS             1059..3320
                     /gene="katE"
                     /locus_tag="ACHOT6_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFP9949212.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3578..4336)
                     /gene="chbG"
                     /locus_tag="ACHOT6_004080"
     CDS             complement(3578..4336)
                     /gene="chbG"
                     /locus_tag="ACHOT6_004080"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFP9949213.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(4349..5701)
                     /gene="celF"
                     /locus_tag="ACHOT6_004081"
                     /gene_synonym="chbF"
     CDS             complement(4349..5701)
                     /gene="celF"
                     /locus_tag="ACHOT6_004081"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFP9949214.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5806..6648)
                     /gene="chbR"
                     /locus_tag="ACHOT6_004082"
     CDS             complement(5806..6648)
                     /gene="chbR"
                     /locus_tag="ACHOT6_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFP9949215.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCLVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6656..7006)
                     /gene="chbA"
                     /locus_tag="ACHOT6_004083"
     CDS             complement(6656..7006)
                     /gene="chbA"
                     /locus_tag="ACHOT6_004083"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFP9949216.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMYQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(7057..8415)
                     /gene="chbC"
                     /locus_tag="ACHOT6_004084"
     CDS             complement(7057..8415)
                     /gene="chbC"
                     /locus_tag="ACHOT6_004084"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFP9949217.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2318 a         2184 c         1858 g         2122 t
ORIGIN      
        1 attctcatta atatctctta tccgaaactc actgccataa agattattaa ttatcactct
       61 atatttaaca ttgtcctgca gatacgtatc aaaactaagt gctggcgctc ctggctttga
      121 ggcatcatag ttaatgttag acatataggc aatcatacga ttgaagttcg actctgctat
      181 ttcatcgcag tcacattgac ccgttgatat aaagaaagtg agtgccgcca tggtagactc
      241 aagcctttct ttaaaagagt cgaaagccag gcttttaata tttaaatcac cataattact
      301 ctgtattaag tttgtagaaa acatctcccg ccccatattg ttaacaaaat tattatctca
      361 tttaaatcta agtcacttac aatataagtt taagagcgac gccacaggat gaactatcaa
      421 aaatagctca tcatgattag caaaacttaa ccattttaaa ataaataaac aattaaagaa
      481 aaaagatcac ttatttatag caatagctcg tcaaaggcag ctttttgtta caggtggttt
      541 gaatgaatgt agcaacgaaa tgcagaattt caggtcatgt aactcccggc aaaaccggga
      601 ggtatataat ccttactcag tcacttcccc ttcctggcgg atctgatttg cccaacgttg
      661 ggcagattca ggcacagtaa acgccggtga gcgcagaaat gactctccca tcagtacaaa
      721 cgcaacatat ttgccacgca gcatccagac atcacgaaac gaatccatct ttatcgcatg
      781 ttctggcggc gcgggttccg tgcgtgggac atagctaata atctggcggt tttgctggcg
      841 gagcggtttc ttcattactg gcttcactaa acgcatatta aaaattagaa aaactgtagt
      901 ttagccgatt tagcccctgt acgtcccgct ttgcgtgtat ttcataacac cgtttccaga
      961 atagtctccg aagcgggatc tggctggtgg tctatagtta gagagttttt tgaccaaaac
     1021 agcggccctt tcagtaataa attaaggaga cgagttcaat gtcgcaacat aacgaaaaga
     1081 acccacatca gcaccagtca ccactacacg attccagcga agcgaaaccg gggatggact
     1141 cactggcacc tgaggacgga tctcatcgtc cagcggctga accaacaccg ccaggtgcac
     1201 aacctaccgc ccctgggagc ctgaaagccc ctgatacacg taacgaaaaa cttaattctc
     1261 tggaagacgt acgcaaaggc agtgaaaatt atgcgctgac cactaatcag ggcgtgcgca
     1321 tcgccgatga tcaaaactca ctgcgtgccg gtaaccgtgg tccaacgctg ctggaagatt
     1381 ttattctgcg cgagaaaatc acccactttg accatgagcg catcccggaa cgtattgttc
     1441 atgcacgcgg atcagcagct cacggttatt tccagccata taaaagctta agcgatatca
     1501 ccaaagcgga tttcctctca gatccgaaca aaatcacccc agtatttgtt aggttctcta
     1561 ccgttcaggg tggcgctggc tctgccgata ccgtgcgtga tatccgtggt tttgccacca
     1621 agttctatac tgaagagggt atttttgacc tcgttggcaa taacacgcca atcttcttta
     1681 tccaggatgc gcataaattc cccgattttg ttcatgcggt aaaaccagaa ccgcactggg
     1741 caattccaca agggcaaagt gcccacgata ctttctggga ttatgtttct ctgcaacctg
     1801 aaactctgca caacgtgatg tgggcgatgt cggatcgcgg catcccccgc agttaccgca
     1861 ccatggaagg cttcggtatt cacaccttcc gcctgattaa tgccgaaggg aaggcaacgt
     1921 ttgtacgttt ccactggaaa ccactggcag gtaaagcctc actcgtttgg gatgaagcac
     1981 aaaaactcac cggacgtgac ccggacttcc accgccgcga gttgtgggaa gccattgaag
     2041 caggcgattt tccggaatac gaactgggct tccagttgat tcctgaagaa gatgaattca
     2101 agttcgactt cgatcttctc gatccaacca aactgatccc ggaagaactg gtgcccgttc
     2161 agcgtgtcgg caaaatggtg ctcaatcgca atccggataa cttctttgcc gaaaacgaac
     2221 aagcagcatt ccatccaggt catattgttc ctggtctgga cttcaccaac gatccgctgt
     2281 tgcagggacg tttgttctcc tataccgata cacaaattag tcgtcttggt gggccgaatt
     2341 tccatgagat tccgattaac cgtccgacct gcccttacca caacttccag cgtgatggca
     2401 tgcatcgtat ggggatcgac actaacccgg cgaattacga accgaactcg atcaacgata
     2461 actggccgcg cgaaacaccg ccgggaccga aacgcggcgg ttttgaatca tatcaggaac
     2521 gcgtggaagg aaataaagtt cgcgagcgca gcccatcgtt tggcgaatat tattcccatc
     2581 cgcgtctgtt ctggctaagt cagacgccat tcgagcagcg ccatattgtc gatggtttca
     2641 gttttgagtt aagcaaagtc gttcgtccgt atattcgtga gcgcgttgtt gaccagctgg
     2701 cgcatattga tctcactctg gcccaggcgg tggcgaaaaa tctcggtatc gaactgactg
     2761 acgaccagct gaatatcacc ccacctccgg acgtcaacgg tctgaaaaag gatccatcct
     2821 taagtttgta cgccattcct gacggtgatg tgaaaggtcg cgtggtagcg attttactta
     2881 atgatgaagt gagatcggca gaccttctgg ccattctcaa ggccctgaag gccaaaggcg
     2941 ttcatgccaa actgctctac tcccgaatgg gtgaagtgac tgcggatgac ggtacggtgt
     3001 tgcctatagc cgctaccttt gccggtgcac cttcgctgac ggtcgatgcg gtcattgtcc
     3061 cttgcggcaa tatcgcggat atcgctgaca acggcgatgc caactactac ctgatggaag
     3121 cctacaaaca ccttaaaccg attgcgctgg cgggagacgc gcgcaagttt aaagcaacaa
     3181 tcaaggtcgc tgaccagggt gaagaaggga ttgtggaagc tgacagcgct gacggtagtt
     3241 ttatggatga actgctaacg ctgatggcag cacaccgcgt gtggtcacgc attcctaaga
     3301 ttgacaaaat tcctgcctga tgggaggcgg cgcaattgcg ccgcctcaaa tgatttacat
     3361 agtgcgcttt gtttatgccg gatgcggcgt gaacgcctta tccggcctac aaaactgtgc
     3421 aaattcaata tattgcagga aacacgtagg cctgataagc gaagcacatc aggcagtttt
     3481 gcgtttgtca gcagtctcaa gcggcgcagt tacgccgcct ttgtaggaat taatcgccgg
     3541 atgcaaggtt cacgccgcat ctggcaaaca tcctcactta cacatcccga taactcccca
     3601 accgataacc acgctgagcg atagcacctt tcaacgacgc tgatgtcaac acatccagct
     3661 ccgttaagcg tgggaaacag taagcactct gacggatagt attatcgata aacgccggat
     3721 gacacatcac ctccagcgaa cgatcacccc gatggcttgc gtcatccagc acttgcagga
     3781 acagtgcctc gctaatctct tcaccataaa acgcgctact gaatccctga gaactgcgca
     3841 gattagccgg taaatcacca gcgttagata gcggctgacg gtcgatgcgc aacgcaatcc
     3901 cctcttcagc cgcaaacctg gcaacaatcg ggaaaatctg cgggaacata tgcacatgat
     3961 gatggctatc aagatgcgta ggtttgcgtc caaatagctc aatgaaacgc aaatactgac
     4021 tggcaagctc ctgagtaatt tcttccagcg gtaaagcatc ttcttctgcc aactgccaga
     4081 tccattttcc cagcacacca tcgcgggtta accccggcat agctgtcagt ggcttgccca
     4141 tagtaaggac aaagtgcatc cctatggcca gactcggttc atcacgactc aactgcaccg
     4201 catggtcaat agcctgccca ttcaccagcg ccgtcgtcga tgtgacaatc ccattgcgac
     4261 aggcctcgat aatgccgtag ttctgacctt tgcttaagcc aaaatcatcg gcattaacaa
     4321 tcagtaagcg ttccataatc agcctcggtt aatgtgcttt tttaagctct gcgatgcagt
     4381 cggcaaaatt tggcagccat ttctcgtgcg ccagaatcat ctcgcgtgcc agcagctcag
     4441 catcgcgatc ggaatgcacc aacggactaa ggtttagcgc cagtaacaca tcgttaaatt
     4501 ctccgctaag tgcggcgttg ctggcagcaa tctcgaagcc tttaatggtg tgaatcagcc
     4561 ccatcacttt atcatcgaaa tgcgtaatgc gcggatgtgg cgtcgcgcca tcgcgcccca
     4621 gcttacaggt catttctacc gcccagtctg ccggaatatt atcaatatgc ccatgatgcg
     4681 ggatattaac gtaatgttca gcttgcttgt cgttgtagat agcgttgatc acttcgcacg
     4741 ctgcatcaga gtaataagcc ccaccgcgct gttccagttc tttcggctta actttcaact
     4801 ccggattttt atacagctca aaaagttgtt tctcgacttt ctgtactacc tgtgctcgtg
     4861 cgccgccttt gtagtattcg cccatttcaa tagccagcat ctctttctgc ttgaagtaat
     4921 acagcagata agaacatggc agcagattta acgaacgaat taagccctca ctaaatggca
     4981 gatcgaaaat attttttaca gaggacgctt ttaactgccc tgacgccaca ccatcaagca
     5041 attcggcaaa gcgcgacttg ccatttacca gcacatcctt aatgaacacc atatggttga
     5101 ggccgaacag atcgatagat aaatcatcgc tgtctttcag catcagaaca tcgcgaataa
     5161 acatcttcat gccgatcgga atattacaca cgccgataaa gcgtttaaat ccggtatgac
     5221 gataaacggc ttcagtgacc attcccgccg ggttagtgaa gttaatcacc catgcattcg
     5281 gacaaagttc ttcgacatct tttacgatgt caaaaatcac cggaatggta cgcagacctt
     5341 taaacagacc gcccgcgccg ttggtttcct gaccaagata accatgactt aatggaatac
     5401 gttcatccag ttcgcgcgcc ggtaattggc ctacgcgcag ttgggtagta acgaaatcag
     5461 catctttcaa tgcttcgcgg cgatccagcg ttttataaag cttcatcggg acgccagcgt
     5521 tatcaatcat ccgttggcag agttcaaaaa tgatatccag tttctcttta ccatcttcga
     5581 catccaccag ccataattcg ctgaccggca attcgtgata acgcttaata aatccttcca
     5641 gtaactccgg ggtatagctg ctcccgccac caatagtgac gacttttaat ttctggctca
     5701 taatttctcc cttcagtaca gaatactgat atctggcata tctgcccccc cggacataaa
     5761 taatccagca acaggacaga tatgtgaatt gtcaggtata acgacttact gattaaattc
     5821 agtcaatttc ttacgatagc tcttaggcgt aaaggacgtt aatttcttaa acgttttaat
     5881 aaacaaactc gggctactat aaccggcctc aaacgcaata tccgtcacgg aatagttggt
     5941 catttccagt tgttttttgg caaaattaat acggatctca ttaataatct gcattggtgt
     6001 tttgccataa tatcgttgag tcgctcgcgt caaatattcc tgtgatttgg ctgacaacgt
     6061 caccatattc tccagcgccg attcactaaa ctgctcttta tcatgcatct tttctaccgt
     6121 acttttcagc cactgcggta catcatcaat cacctgttct tcgcggtaat gacgtaaacg
     6181 gttaatgaca taaaaagtaa ccatctcaac aaactcttcc agccctgttt cgcggaaatt
     6241 caatgaagaa atcactgttt ccacataggt gagaaaagcg ttattggtcc ggtatacctg
     6301 cgaagcgaca aggcaataag gcaacaacgg caggtaatgc tgctcaaaaa agcgtttact
     6361 gatcccaacg ttcaatatac gcgtggcacc aaactcataa aaactttgat ggtgcgaacc
     6421 taacggaata aaaacaaaat cgccccgttc cagtaacacg cgcttaccgt taatctcctg
     6481 gaaataacgc ccggttaata ccagagtaaa ttcataatag tcgtgctgat gcagcccgct
     6541 gatactctca gttttgttat agataaacac atggaagttt ttgccattaa acaactgctg
     6601 ttctcgggca gtggcaattt ccggcgcgtt aatcactggc tgcatcatcg actccttatg
     6661 ccttcagttt ttcatgaagc tcaattaatt cagtaatcag ttcacgcgca agcatggacg
     6721 tcattaaatg atcctgagcg tggaccagca ccagactcac tttcatctta ccttcgcccg
     6781 catcgccttc aatcagtttc gtctgtacca gatgcgcttc attcaatgcc atccgtgact
     6841 gatacatcat ggcttttgct gcagcaaaat cgccctgctt cgcctgtttc agtgccgcat
     6901 acgccaggct gcgcgcttgt ccggagttga tgataagccc catcaccact tcttccagct
     6961 cttcagcttc tgtttgcgta tcaggaatat tatcgagatc catcatacat cgttcctctc
     7021 tcttcttacc ggcaagatta cccgtaccgg catcgattaa aatttcaggg cgttagcgat
     7081 atcttcttcg ctctcttctt tatcaatcgc attttgtgct ttgttagcca ccacaacaaa
     7141 gggcagataa attaaggttg cgatgccaag gttgaagagt gcgaccagca atgcggcgac
     7201 gctaccgttg gtgttaaaga aggctcccag accggttggc atggtccacg gtgcaatatt
     7261 ggtcaccgga ggaatgatgc ccatgtagta cgctgcgagg gtgattgccg ccagaatcgg
     7321 ttgtaccagt acaaacggga taaacatcac cgggttcatg ataattggca gaccaaacag
     7381 aatcggttcg ttaatctgga agatgccgga cggcagcgcc agttttgcca cctgacgata
     7441 atcagcacgg cgagaggcga taaagatagc caggatcagg cctaaagtcg caccactgcc
     7501 cccaaggaaa ataaaggagt ccagcatcgg cttggcccag atatggaagg tcttaccggc
     7561 tgccagcgcc gcttcgacgg aaccatattg ctgataggtc gcgatatttt ccagcgccca
     7621 cggcgtcata atgccgttgt ccagtgcggt cagcgccagc gcgccatgaa taccgaagaa
     7681 ccagagcagt ggaacaaaga tcacataggc ccagcccacc acgctaccca acgatgccag
     7741 tggggttgag atggtatcca taatgatctg atggaagttg gtgccccagg tattcaacgc
     7801 ccaggcaata atccccatca cggaaagaat aataaagccg ggaattaatg ccgagaagga
     7861 acgcgatact gaagcaggta cgctgtcggg cagtttaatg acccaattgc ggcggacaat
     7921 aaaggtaaac atttctgcca ccaccaggcc aataataatc ccggagatga tattcgcccc
     7981 acctaaccag tttgcgccaa ccgcataggc ctcaccgaca ctatatgggg tgacggtcat
     8041 aaatgctgca acggataaca acccagccgc cagcgcatcg actttacgct cttctgccag
     8101 cgccatgcca ataaagaacg gtgccattaa agacattatc cctaatgttc cgttatatac
     8161 gttgccgcca atacctttca gaccattaag tgtttcaatg gttgaggcgt cgaggcgaat
     8221 acctaaggaa taaaaaaacg acccctcccc aaagcttaga aaaacgttgt taattaatac
     8281 aaacatggcc ccggcaaggg ttaacggcat taagcgaata aagccatttt tgattgcatt
     8341 aacgtgtggc tgctttccta ttttaactgc aaaagggagg agtacctttt caagcgatgc
     8401 aataacatta ctcatagaaa aataccctta aaaaccgcaa tttaaatatt gcggtattga
     8461 tttatgaaat aactctttga cg
//
LOCUS       DAZKIV010000172         8431 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZKIV010000172 DAZKIV010000000
VERSION     DAZKIV010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.172"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..290
                     /locus_tag="ACHOT6_004085"
     CDS             <1..290
                     /locus_tag="ACHOT6_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949218.1"
                     /translation="FNTNKLTRDDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNW
                     NSTYIYKSRHEILPVNLTPETLFGSKSQDKHALFPIFTASWRATRIKNKGI"
     gene            513..1010
                     /gene="ubiC"
                     /locus_tag="ACHOT6_004086"
     CDS             513..1010
                     /gene="ubiC"
                     /locus_tag="ACHOT6_004086"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFP9949219.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1023..1895
                     /gene="ubiA"
                     /locus_tag="ACHOT6_004087"
     CDS             1023..1895
                     /gene="ubiA"
                     /locus_tag="ACHOT6_004087"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFP9949220.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLISNREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2050..4473)
                     /gene="plsB"
                     /locus_tag="ACHOT6_004088"
     CDS             complement(2050..4473)
                     /gene="plsB"
                     /locus_tag="ACHOT6_004088"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFP9949221.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4644..5012
                     /gene="dgkA"
                     /locus_tag="ACHOT6_004089"
     CDS             4644..5012
                     /gene="dgkA"
                     /locus_tag="ACHOT6_004089"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9949222.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5122..5730
                     /gene="lexA"
                     /locus_tag="ACHOT6_004090"
     CDS             5122..5730
                     /gene="lexA"
                     /locus_tag="ACHOT6_004090"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFP9949223.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5803..7128
                     /gene="dinF"
                     /locus_tag="ACHOT6_004091"
     CDS             5803..7128
                     /gene="dinF"
                     /locus_tag="ACHOT6_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFP9949224.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEILKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7244..7453
                     /gene="yjbJ"
                     /locus_tag="ACHOT6_004092"
     CDS             7244..7453
                     /gene="yjbJ"
                     /locus_tag="ACHOT6_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFP9949225.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7495..8010)
                     /gene="zur"
                     /locus_tag="ACHOT6_004093"
     CDS             complement(7495..8010)
                     /gene="zur"
                     /locus_tag="ACHOT6_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFP9949226.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8328..>8431
                     /gene="yjbL"
                     /locus_tag="ACHOT6_004094"
     CDS             8328..>8431
                     /gene="yjbL"
                     /locus_tag="ACHOT6_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFP9949227.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDAIKDKLDNRG"
BASE COUNT         1951 a         2041 c         2275 g         2164 t
ORIGIN      
        1 cctttaacac taataaatta acacgagacg ataatatatt tatcctgttc tcctctgtcg
       61 atgatgggcc tgttatggtg gtaagctccc agcgcttaca tgatatgttg aatcctacaa
      121 aagataccaa ttggaattcc acgtatatct acaaatccag acatgagata ttgcctgtta
      181 atcttactcc ggaaacactt ttcggctcca aatctcagga taaacatgca cttttcccca
      241 tttttactgc gagttggcga gctactcgta taaagaataa aggtatttaa gtaaaggaaa
      301 acatcaccat tcctggcatc atgggcggtg atgcccctat ggttgccctc gccagcacgg
      361 gcatcggcaa agcgtaaggt tcaacagcgt tttaccactt catgcgattg ttgcgttttt
      421 gttgcgcatt agatcactta atttgcttta catctcccgt aaacactttt ctgcgataca
      481 atgcctttac gttatgtaac ggagagttcg gcatgtcaca ccccgcgtta acgcaactgc
      541 gtgcgctgcg ctattttaaa gagatccctg ccctggagcc gcaactgctc gactggctgt
      601 tgctggaaga ttccatgaca aaacgttttg aacagcaggg aaaaacggtg agcgtgacga
      661 tgatccgcga agggtttgtc gagcagaatg aaattcccga agaactgccg ctgctgccga
      721 aagagtctcg ttactggtta cgtgaaattt tgttatgtgc cgatggtgaa ccgtggcttg
      781 ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc ggagctggcg ttacaaaaat
      841 tgggtaaaac gccgttagga cgctatctgt tcacatcatc gacattaacc cgggacttta
      901 ttgagatagg ccgtgatgcc ggactgtggg ggcgacgttc ccgcctgcga ttaagcggta
      961 aaccgctgtt gctaacagaa ctgtttttac cggcgtcacc gttgtactaa gaggaaaaaa
     1021 atatggagtg gagtctgacg cagaataagc tgctggcgtt tcatcgctta atgcgtacgg
     1081 ataagccaat tggcgcgtta ctgctgctct ggccaacatt atgggcgttg tgggtggcga
     1141 caccgggcgt tcctcagctc tggatcctgg cggtgtttgt cgcgggtgtc tggctgatgc
     1201 gcgctgccgg atgtgtggtg aatgattatg ctgaccgcaa gtttgatggt catgttaagc
     1261 gcacggcgaa ccgaccgctt cccagcggcg cggtaacgga gaaagaggcg cgcgcgctgt
     1321 ttgtagtgct ggtattgatt tcatttttac tggtgctgac gctgaatacg atgactattc
     1381 tgttgtcgat tgccgcgctg gcgctggcgt gggtataccc atttatgaag cggtataccc
     1441 atctaccgca agtggtgctg ggcgcggcgt ttggctggtc gattccaatg gcttttgccg
     1501 ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt cctcgccaat attctctggg
     1561 cggtggctta cgacacgcag tatgcgatgg ttgaccgcga tgatgatgtg aagattggca
     1621 ttaaatccac ggcaatcctg ttcggccaat acgataaatt gattattggt attttgcaga
     1681 ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa tggcttaggc tggggatatt
     1741 actggtcaat tgtggtggct ggcgcgctgt ttgtttatca acaaaaactg atttccaacc
     1801 gcgagcgtga agcctgcttt aaagcattta tgaataataa ctatgttggt ctggtactat
     1861 ttttagggct ggcaatgagt tactggcatt tctgatgatg taaaaaagcc ggatgatcat
     1921 ccggctttct tctgggttgc ctgatgcgcg gcgcttctca ggcctacaca acacatcgca
     1981 atttattgaa tttgcagatt atggaaggcc ggataaggcg ttttcgccgc atccggcaat
     2041 tctctctgat tacccttcgc cctgcgtcgc actctcaatc gtcaaacgca cgtctgatgt
     2101 aatcaactcc gccagcaact gataaacctt catcgtttct gccggttcgg catcgccgct
     2161 atcgctgata tacccttcat cacgcagcgt cagcaccaga gaactgaaca ccgccttgtc
     2221 gaagaactcc ggcgcgttga tgccgtgcag cacggagaga cgttgcgcga cggtgcggct
     2281 ctctttctcc agcgtaccgc ggttgatcga cgggttggca ctcaacaacc agaaggtgat
     2341 ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc agctgtagcg tgcgagaatg
     2401 cgccgggttg atatgcaact catcatcttg cagggtaatc agcccctgac gttgcatctc
     2461 atttgccagc gcatcaataa cgtccggcaa ctcgtcgcga tcccagcgca ggaacagctc
     2521 cgctttcagc attgggtaaa gcacattgac gtgctccatc aatacgtcgc gggagatgtg
     2581 gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc accaacatat gcgcaatgtt
     2641 gttgcgatag taggtcatca gcaccgcttg ctcgcgtggc agaatgatga tgtcgccgat
     2701 agtgtctttc tcgacttcaa acttgttcat ttgcagcgcg tgatcgataa gctcgctggc
     2761 gctggctgaa ggaacggtag agtccgtgga gtaaggtacg ttgcgcatca gatccaggta
     2821 gcagttgagt tgctcggtta actgctcgcg ggtgagtgag cgctgacgtg atgccagaag
     2881 cgcagtacaa cacaggttca tggcgtttgc cgcgcctgcg ttgttaatgc gtaccatcag
     2941 atcggcagca atattattga ccgtcggcgt taaccaggcc ggacgcaccg cttcgatggg
     3001 atcgatagat tcacgccagt ctggtacgtg ctggttaagg taggtcatca acggcattgg
     3061 ttcaccgaag ttgacgtaac cctgaccgag attacgcagc ttgcttaaac cgcgcagcat
     3121 ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt tctttggcgt aagtacccac
     3181 ttccatgacg tgctcataac cgatgtagat cggaatcagc gtaatcggac gcgtgccgcc
     3241 acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt ttcggatcca gcaaacgccc
     3301 cgtacgggaa cgaccgcctt ccacgaagta ctcgacggaa taaccacggc tgaacagttc
     3361 gccgagatac tcccggaaaa cggtagaata aagtttattg cctttaaacg tacggcgaat
     3421 aaagaacgcc cccagacggc ggaaaatcgg cccggccggc cagaaattca ggttgatccc
     3481 ggcggcgata tgcggcggca ccagcccctg gtgatacagc acgtaagaaa gtagcaggta
     3541 gtccatgtga ctgcggtggc aaggcacata cactagctca tgaccgtcgt gggccagctg
     3601 gcgaacgcgc tcagcgttat ggacgttgat gccctggtaa agtcggttcc aggtgaagcc
     3661 cagaatacga tcagtcaggc ggatcatctc gtaagagaaa ttcgccgcaa tctcttccat
     3721 cagcgcaatc gcgttctgct gcgctttttc atgggagatt tttttgctgc gcgcttcatc
     3781 ttctaccgct ttggcaatgg cgcgggaggc gagcagctta ttaaacagat cctgacgagc
     3841 aggcagacgt gggcctacgg cagccagacg ttgacgggca aagtgcatac gcgccacgcg
     3901 cgccagtttc tgagcgatag ttttatccgt gccgtgttca tccgccatac ggcgcagcga
     3961 cactgacggc gagaaacgca caaaactgtc gcgaccgagc cacagtacgg cgaaaaattt
     4021 ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg cctttttcac gccccggcgc
     4081 acgaccaaac atcaccgata ctggcaccat ctgcacatcc agatttgggt tgctgcggtg
     4141 caaatcgaga tagtcgtgga atagcttaat agactcttct ttcggcgtgt aataggtgaa
     4201 cacacgcggc ccgccgtgaa tgaacacata gcgcggcagt agcgtgccgt cgatttccag
     4261 cggctctaac gggtcaggca agtcatgtgc cagacactgg gcgcgcaacg tcagcaaatc
     4321 tgctttcgag ttgtacggta aaacgtacat aattggacga gaggtatcca gccccagttc
     4381 cggggcagga tctgccggaa tagacttgct ttttaccagg atgcttaatg gtaaattcag
     4441 taatttgtag taaattcgtg gccagccgga cataaacgat gtaaagcctc tggttaataa
     4501 tgcaaatgcg cggcaaggat agcagaaagt catgggaaat tctgtggtat ccgctcatgt
     4561 ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac ggtacactga tattgacgct
     4621 cataatgtaa aaaggttctt tcaatggcca ataataccac tggattcacc cgaattatca
     4681 aagctgctgg ctattcctgg aaaggtttac gcgctgcatg gatcaacgaa gcggcattcc
     4741 gtcaggaagg cgtagcggta ttgttggcgg tggtcattgc ctgctggctg gatgtggacg
     4801 cgattacccg cgtgctgctt atcagctccg tgatgctggt gatgattgtg gaaatcctca
     4861 atagcgccat cgaagcagtg gttgaccgaa ttggctctga ataccatgag ctttccggac
     4921 gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat tatcgtcgcc gtgataacct
     4981 ggtgcatcct gttatggtcg cattttggat aacccttcca gaattcgata aatctctggt
     5041 ttattgtgca gtttatggtt ccaaaatcgc ctttcgctgt atatactcac agcataactg
     5101 tatatacacc cagggggcgg aatgaaagcg ttaacggcca ggcaacaaga ggtgtttgat
     5161 ctcatccgtg atcacatcag ccagacaggt atgccgccga cgcgtgcgga aatcgcgcag
     5221 cgtttggggt tccgttcccc aaacgcggct gaagaacatc tgaaggcgct ggcacgcaaa
     5281 ggcgttattg aaattgtttc cggcgcatca cgcgggattc gtctgttgca ggaagaggaa
     5341 gaagggttgc cgctggtagg tcgtgtggct gccggtgaac cgcttctggc gcaacagcat
     5401 attgaaggtc attatcaggt cgatccttcc ttgttcaagc cgaatgctga tttcctgctg
     5461 cgcgtcagcg ggatgtcgat gaaagatatc ggcattatgg atggcgactt gctggcagtg
     5521 cataaaactc aggatgtacg taacggtcag gtcgttgtcg cacgtattga tgacgaagtt
     5581 accgttaagc gcctgaaaaa acagggcaat aaagtcgaac tgttgccaga aaatagcgag
     5641 tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca ccattgaagg gctggcggtt
     5701 ggggttattc gcaacggcga ctggctgtaa catatctctg agaccgcgat gccgcctggc
     5761 gtcgcggttt gtttttcatc tctcttcatc aggcttgtct gcatggcatt cctcacttca
     5821 tctgataaag cactctggca tctcgcctta cccatgattt tctccaatat caccgttccg
     5881 ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg atagcccggt ttatttgggc
     5941 ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta tgctgttgct gtttttacgc
     6001 atgagcacca ccgggctgac tgcgcaggct tatggtgcca aaaatcctca ggcattagcc
     6061 cgtgcgctgg tgcaaccgtt gctgttggca ttgggggctg gggcgttaat tgcgctgctg
     6121 cgtacaccta ttatcgatct ggcgctgcat attgttggcg gtagtgaagc agtcctggaa
     6181 caggcgcggc gctttcttga aatccgctgg ttaagcgcac cggcgtcgct ggcgaatctg
     6241 gtattactcg gttggttact cggcgtgcaa tatgcccgtg cgccagtaat tttgttagtg
     6301 gtcggcaata tcctcaacat tgtgctggat gtctggctgg tgatggggct gcatatgaac
     6361 gtgcagggcg cggcgctggc gacggttatt gcggaatatg caacattgct gattggtctg
     6421 ctaatggtgc gtaaaatcct caaactacgc ggaatttccg gcgaaatact gaaaactgcc
     6481 tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg atatcatgct gcgttcgctg
     6541 ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg cgcgactggg gagtgacatt
     6601 atcgctgtta acgcggttct gatgacgcta ctcaccttta ccgcctatgc gcttgatggt
     6661 tttgcctacg cggttgaagc gcactccggt caggcatacg gtgcgcgcga cggtagccag
     6721 ttgctggatg tatggcgggc agcgtgccgc cagtcgggga ttgtagcgtt actgttttcg
     6781 gtggtttatt tgctggcagg ggaacacatc attgcgttgc tgacgtcgtt aacccagatt
     6841 cagcagctgg ctgaccgcta tcttatctgg caggtgattt tgccgttggt tggcgtctgg
     6901 tgttatctgc tggacggcat gtttataggc gcaacgcgtg ccgccgaaat gcgtaacagt
     6961 atggcggtgg ccgccgcagg ttttgcgctg acgctcctta cgctgccgtg gctgggtaat
     7021 catggtttgt ggctggcatt aaccgtcttt ctggcgttgc gcgggctttc tctggcggct
     7081 atctggcggc gtcactggcg caacggtacc tggtttgccg caacgtgacg gttaaaaatt
     7141 ctgaataaat tatcctaagc caaattgctg actacactta atctcacgtt cagaagaaaa
     7201 gtgaacgtac tctcattcac aacctaacga tgaggtcttg attatgaata aagatgaagc
     7261 cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag caatggggca aactgaccga
     7321 tgatgatatg acgatcattg aaggtaaacg tgatcaactg gtcggtaaaa tccaggaacg
     7381 ttatggttat cagaaagatc aggcagaaaa agaggtcgtg gattgggaaa cccgcaatga
     7441 atatcgctgg taattaatcc ctcctgcccg acgtgtacaa ggatgtacgc cctcttaacg
     7501 cggtttcttt ttcacctgca cagagtgatc atgctggcac tgttcaggat gacgacacgc
     7561 ttccacttcc acgcatgccg cacagagccc atgtgcttca atcacattat gccgcagggc
     7621 aaaccccatt tttgccgcca gcgtatgcat aatgtcttcc acgccttctg cacactcttc
     7681 tttcaccgcg ccgcagcgat cgcatataaa catggctgac gtatgcgtgg gctggtcgaa
     7741 cagatgacag agcacataac tgttggtgga ttccacctta tgcacaaaac cttgctcaag
     7801 cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct tgcggttcag cttcgcgcag
     7861 taaatcaagc agatcataag cgctgatagc gccatcctgc agactcatca ggcgcaacac
     7921 ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg cagatttttt cagcctgcgc
     7981 taataactcc tgcgttgtgg tcttttccat cgggcacctc aaagtggggg ttaagaatgt
     8041 ccttacttta ccatgttcca ggaaaaacac cgagaaccct tattgttgcc gtaatgttga
     8101 ttttctgttt tgtaggtaag gtgttgtgtt gccttgtcgt accattatca acacgataat
     8161 aattaataat tcatttttaa ataaattgct tcaagttaac tcgctattta tttaactggg
     8221 tttaaacgca tacctgtagt tcagaaatta tgagcgattt ttgatagtga ccagatggca
     8281 aaagaattat aaggaggaca cggtacgaca aaaaaggtag atgaatcatg ttgaagataa
     8341 tacccggtgc aactggatac tttaataaaa cactcaattc aaatcaattc gataatgaag
     8401 atgcaatcaa agataaatta gataatagag g
//
LOCUS       DAZKIV010000173         8420 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZKIV010000173 DAZKIV010000000
VERSION     DAZKIV010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.173"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..393
                     /locus_tag="ACHOT6_004095"
     CDS             <1..393
                     /locus_tag="ACHOT6_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin immunity protein"
                     /protein_id="HFP9949228.1"
                     /translation="GIKKRDNSRDKTKEHKHMNRKFYFKNILWGMCPIIISILHLIYD
                     TPTEASIKVDLFLILSGLALPCSIYLTKTITLKYTSISFWKKPLFWSYGIYHFHLVYI
                     MICIVLAIPSAIIVLFFHFYQNITRNWK"
     gene            complement(878..1018)
                     /locus_tag="ACHOT6_004096"
     CDS             complement(878..1018)
                     /locus_tag="ACHOT6_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9949229.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1320..1583)
                     /locus_tag="ACHOT6_004097"
     CDS             complement(1320..1583)
                     /locus_tag="ACHOT6_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9949230.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2008..2133
                     /locus_tag="ACHOT6_004098"
                     /pseudo
     CDS             2008..2133
                     /locus_tag="ACHOT6_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            2198..2458
                     /locus_tag="ACHOT6_004099"
                     /pseudo
     CDS             2198..2458
                     /locus_tag="ACHOT6_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2508..2747
                     /locus_tag="ACHOT6_004100"
                     /pseudo
     CDS             2508..2747
                     /locus_tag="ACHOT6_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(2795..3412)
                     /gene="ureG"
                     /locus_tag="ACHOT6_004101"
     CDS             complement(2795..3412)
                     /gene="ureG"
                     /locus_tag="ACHOT6_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFP9949231.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3424..4098)
                     /locus_tag="ACHOT6_004102"
     CDS             complement(3424..4098)
                     /locus_tag="ACHOT6_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFP9949232.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(4099..4563)
                     /gene="ureE"
                     /locus_tag="ACHOT6_004103"
     CDS             complement(4099..4563)
                     /gene="ureE"
                     /locus_tag="ACHOT6_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFP9949233.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNSHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(4573..6276)
                     /gene="ureC"
                     /locus_tag="ACHOT6_004104"
     CDS             complement(4573..6276)
                     /gene="ureC"
                     /locus_tag="ACHOT6_004104"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFP9949234.1"
                     /translation="MSNISRQAYADMFGPTTGDKIRLADTELWIEVEDDLTTYGEEVK
                     FGGGKVIRDGMGQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNP
                     DIQPNVTIPIGVSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTG
                     PTAGSNATTCTPGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKI
                     HEDWGATPAVINCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGA
                     GGGHAPDIITACAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAE
                     SRIRQETIAAEDVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEES
                     GDNDNVRVKRYIAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKG
                     GMIAMAPMGDINGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLH
                     STTAVVKGCRTVQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFL
                     F"
     gene            complement(6269..6589)
                     /locus_tag="ACHOT6_004105"
     CDS             complement(6269..6589)
                     /locus_tag="ACHOT6_004105"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFP9949235.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFELGQKREVELVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(6598..6900)
                     /locus_tag="ACHOT6_004106"
     CDS             complement(6598..6900)
                     /locus_tag="ACHOT6_004106"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFP9949236.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(6910..7689)
                     /locus_tag="ACHOT6_004107"
     CDS             complement(6910..7689)
                     /locus_tag="ACHOT6_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFP9949237.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYPGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            8070..8345
                     /locus_tag="ACHOT6_004108"
     CDS             8070..8345
                     /locus_tag="ACHOT6_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949238.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIIIFPDDIIP"
BASE COUNT         2085 a         2114 c         2089 g         2132 t
ORIGIN      
        1 ggaataaaaa aaagagacaa ttcccgagat aaaacaaaag agcataaaca tatgaaccgg
       61 aaattctatt tcaaaaatat cctgtgggga atgtgcccca ttataatatc catattgcat
      121 ctgatatatg acacaccaac agaagcatcg ataaaagttg acttatttct gatactcagt
      181 ggcctggccc ttccttgttc aatatacctg acaaaaacaa tcacactaaa atacacatcc
      241 atatcattct ggaaaaaacc tcttttctgg tcttatggta tatatcattt tcatcttgtt
      301 tatataatga tatgtatagt tttagcaata ccttctgcaa ttatcgtttt attctttcac
      361 ttttatcaaa acatcaccag aaactggaag tgacaaccgc aataatgaac ataaaaaggc
      421 aaatcagaat ggcagtaaat caataaagta ccatttcata tgaatcatca gaaaaacggt
      481 ggacgcgaca tccagcacga ggcaaaacac agcaacaaaa taagccggat atcgcgccct
      541 gtacttttct aactcaactc ccggcattgt gcaagtttta atcgcaggtg gattcagagc
      601 cacgccccca ttgattcagc ctgaagacac gtatatcctt ggagaaagca ggaatctcat
      661 tgctgtgaaa ttccttacag aatcttcagc cccattttat ggggcttttt ttggtgcccc
      721 cacgctgtta ccacactttc gggatgcgaa taaaaaagcc cacagttttt acactgcgga
      781 cttttgtttt ttctttgtga tttgtgtgca agggtgcgac tttcgaccga acctttggca
      841 accggatagc ggggactgtt gtccccgctg cggtttctta cggtttacac tgcaagaacg
      901 ccgcaatttc cgaccacatg aagcttaccc tgaattcaca cggtgatgcg tgtaacaccc
      961 agacggtcag caacatggtc aggcataaca tgcccacaat gatcgttttt tgcggcatag
     1021 cacttgctcc ttttacagag aggcgctaac cttccacttg ctgaaggtag atggtcaggg
     1081 cctcgggtta acgtttaagt tgactcgggg cctttccaca tccggccttc aggtaatcct
     1141 tccagccgtc agccgaaagg cacccgcgcg taatgtactg gttttcagag aggtccgcaa
     1201 tcttccggtg acgagatcat ctgcacacgc cccggtggga cgggctaacg gtatattcag
     1261 gtgcaggatc tctctgtctg gtgcgttttt aaattgtcaa acgcaccctg gcggcaaaat
     1321 cagccacctt ttttcgcatc aacaaaacgc ccaaaacgct gggtaaagcg tgcaacatta
     1381 ccttctgaag cgacagttct cagtttacct gtataaaacg gatgcgactt agaagaaacg
     1441 tcaattgtaa catatgggta agttacacca tccagttcaa ttacccggtc agttttgatc
     1501 gtcgaaccga tcctgaagta ctcatcaata ctggtatcgt gaaatactac tgtacggtat
     1561 tccggatgga tattcggttt cacaacagtt ttcccccttt aagtgaaaat tccgcataaa
     1621 ggtacatatt cagacggtca gattcaactg tctgttctta taacctcact ctctttcagt
     1681 attacaatat gttatataat tataaactgg acttcccgaa aaaatccatt aaataagaag
     1741 cgagccgttg cggaggcttg ctctctcctt taacctcatg tgcaatatcc gtaacgcgct
     1801 cacataaatc atcatctgat aatataacaa cacccattat tttattctga cctaaaaact
     1861 tcttatgaaa taaacgttta cccgacgtat ctgatcccgc tgtttttacc tgtatccttt
     1921 aacatacttg agaagcccgc tgaaaaaata taccgtaaag gcggtttctc tgtcttccat
     1981 aaacctgtcg gataagaagg gcattaaatg aaaaccaaaa aagttatgat gattgcactg
     2041 gtcagctctg cgctggctct ttcaggatgt ggagccatgg gcacagcgat caaaaaacga
     2101 aatctggaag taaaaaccca gatgagtgaa acctgaggta gcctgagttt aacggacact
     2161 ccttcctgaa atagaatggc atcagaagga gctaataatg agcagaaaaa cccaacgtta
     2221 ctctaaagag ttcaaagccg aagctgtcag aacggttctt gaaaatcaac tttcgatcag
     2281 tgaaggcgct tcccgattat ctcttcctga aggcacttta ggacaatggg ttaccgccgc
     2341 cagaaaaggg ctcggtactc ctggttcccg cacggtggct gaactggaat ctgaaattct
     2401 gcaactgcgt aaggcgttaa atgaagctcg ccttgagcga gatatattaa aaaaagcagc
     2461 tgtagattca atctgtcaat gcaacacccc tttcaattat ctctttcact gcccgcgacg
     2521 gtatcggtta tcagtttgac ctgctggcta acggtgaaac cgtactgcgt gatgcatccg
     2581 gcgaaaaacg catcaagggt gaaaaagacg gtcgtatgct catgaatgtt ggcctgaatg
     2641 cggaaattcg cgacaacatc cgcttcggtc tggagtttga aaaatcggca tctggtaagt
     2701 acaacgtgga taacgcggtg aacgccaact tccgttattc cttctgataa aaacaagggg
     2761 ccgcctgccg gccctgatac agaggatgtt gtgattactt acccagcatg ccctgctctt
     2821 cgataaaggc gatgatgttt tgcagacctt caccactttt caggttagtg aacccccatg
     2881 ggcgttctcc gcgcatacgt aaagtatcac gctccattac ctcgagggag gcgcccacgt
     2941 atggtgcaag gtcagtttta ttgatcacga ggaagtcgga tttggtgatc cccggaccgc
     3001 ctttacgcgg gatcttttct ccttctgcca catctatgac atatatggta aggtccgcca
     3061 gttcggggct gaaggtcgca ctcaggttgt cgccgccgct ttccacaaag atcagatcga
     3121 gattaccaaa tttctcactc agtgcttcca cggctgccag attcattgat gcgtcttcac
     3181 gaatggctgt atgagggcag ccccccgttt cgacaccgac aatgcgttcc gcttccagtg
     3241 ctccggcctc agtgagaatg cgttgatctt ctctggtgta gatgtcgttg gtgactacgg
     3301 ctaattgcca ggtgtcacgc atggatttgc agagggcttc cagaagcgcg gttttacctg
     3361 agccgaccgg accgccgacg ccgacacgca gcggttgctt gattatgttc attttatttt
     3421 ctcctaagaa cggaataatc gggaatactg ggtttcatgt cgcgcggaag cgatagatgc
     3481 aagtggcgtt gctgaaccaa ttgcgtgatc cggggttgca agggcactgt ccatgttcgc
     3541 cgcatagcgg gcacacaggc gcataattaa ctgctgggca gccagctggc cgtagggtac
     3601 cagcctgacg cccgccatca ctgcgctttc aatccagctg tagccaaggc tcagggccag
     3661 gtctgaaacg gatatttgcc agcgtacgcc gagccatgcc ataccggtta actggctttg
     3721 ctggcatagt ttgcgccatt cggccgggca gtcgggctgc cagtcgacca gaagtctggt
     3781 gaaggctgca ccgcggttac gttcttcatc gcgtagctca cgggtttccc tgcaggccag
     3841 cagataagcg gtccagcgcc cggcactgtc aggatcgccc gcttcacagg cccggtatag
     3901 ccgggcaagc agtggcaggt cgacggcaaa aaaactctgt tccatctgca actgctgcca
     3961 gtgttcaaag gcggcgctat cttctaccca gcctgcttcc actgcccact ccagtccctg
     4021 ggaccagctg tagcctccaa cagggaggct gttactggca agctgcatca gccgcagacg
     4081 tttttccgga gtcggcattc agtggctgtg ctcctgatcg tgattatggg cgtgcgactt
     4141 gctggcgtaa gcgccggctt ccggctcaaa cggaagatgt gcaaaatcaa catccagccc
     4201 aaactggcgc agcatatcat cgaggacgtg atcgtgatgg tagcgcagct ctccgggcat
     4261 tatttgcagc ggaacgtggc tattgccaag atgccagcag gctttcgcga gcataaaagg
     4321 atctgcgcag cgcactacag aaacggcttc atcagcggcg atcaccttaa tgaattcatc
     4381 accgttttcg ttactgagaa tatcaccatc gcgcagcagc agaccgcgtg gcagcaacag
     4441 tccagcctga cgtccatcgt taagggtgac tttaatacgg ctttttaccc gcatgtcaac
     4501 agggagggta acgctggcag ttgtctgagc cggggtctcc acccgccggg tcaaataaag
     4561 catttgtgct ccttaaaaca ggaaataacg ttgcgccatt ggcagaatgt ccgccggttc
     4621 actggttatc agttcgccgt tgatacgcac ttcgtaggtt tgtgaatcca cggttatatc
     4681 aggcaacagg ctgttgtggc gcatatcggc tttttgtact gtgcggcagc cttttaccac
     4741 agcagttgtg ctgtgcaggt taagctgttc agcgacgcca tttgctgctg ctgcctgcga
     4801 caggaaagtc acacgacagc ggtgacgggc actgcccaat gcagcgaaca ttgggcgata
     4861 gtgcaccggc tgcggtgtgg ggatagagcc gttgatatca cccatcggcg ccatggctat
     4921 cattccgcct ttgacgatag tcgccggttt tacgccaaag aacgccgggg accagagcac
     4981 cagatccgcc agttttccca cttcaatcga gccgacttca tgagcaatac cgtgggttaa
     5041 tgccggatta atggtgtatt tagcgatata gcgcttcacg cggacgttgt cgttatcacc
     5101 actttcttcc ggtaacgggc cgcgctgaac tttcatccgg tgtgccacct gccaggttcg
     5161 taacactact tctccgacgc gtcccatggc ctgcgaatcg gacgaggtga gggagaacgc
     5221 gccaaggtcg tgcaggacgt cttccgcggc aatggtttcc tggcgaatgc gcgattcggc
     5281 aaaggctacg tcctcggcga tatccgggtc aagatgatgg caaaccatca gcatgtccag
     5341 atgctcatca atagtgttga cggtataggg tagcgtcgga ttggttgagg aaggcagaat
     5401 attggggtgc gcgcaggcgg tgataatatc cggagcatga ccaccacctg caccttctgt
     5461 atggaaggta tggatagtgc gcccgccgat ggcagtcaga gtatcctcaa cgaatcctga
     5521 ttcgttaagc gtatcactgt gtagcgcaac ctgaacgtcc atttcgtcgg ctacagtcag
     5581 tgcgcagttg attaccgcag gtgtagctcc ccagtcttcg tgaattttga ggccgataac
     5641 cccggccgcg acctgctcac gaagcgcatc cggattggag caattgcctt tacccagcaa
     5701 cccgatattg accggcaggc tgtctgcagc ctgcagcatt tgataaatgt accatggtcc
     5761 tggggtacag gttgtggcgt tagaacccgc tgtcgggcca gtaccgccac cgatcatggt
     5821 ggtaatgccg gatgtcagcg cttcttcagc ctgctgtggg cagatccagt gaatatgcgt
     5881 atcgacgcca cctgcggtaa cgatcctgcc ttctgcggca ataatttccg tggatacgcc
     5941 gattgggata gtgacgttgg gttgtatatc aggattaccg gctttgccga tagcaaatat
     6001 ccttccatct ttgacgccga tatcggcttt aacgatcccc cagtaatcga tgatcagggc
     6061 attggtcagc accagatcag cgcagccggc ggagagcatt tgcccctgtc ccataccgtc
     6121 gcggattact ttaccgccgc cgaatttgac ctcttcgccg taggtagtta aatcatcttc
     6181 gacctcgatc cacagctcag tgtctgccag acgaatttta tcaccggtgg tagggccgaa
     6241 catgtcagca taggcctggc gtgaaatatt actcatcatt tgcctccagc tcacccatta
     6301 tatctccgcg gaaaccgtgc acgatacggg caccgctcag cgccaccagt tcaacctcac
     6361 gcttttggcc tagttcgaag cgcactgcag ttccggcggc gatattgaga cgatagcctc
     6421 ttgccttctg gcgatcgaac ttcagtgccg ggttaacctc cgcgaagtga taatgggaac
     6481 cgacctgaat tggtcggtct ccgtgatttt caacgataat actgcaggta gcccggccga
     6541 tgttaagctc tatatagcca ggtttaacct tatattctcc agggatcatc tgttacctca
     6601 gattatcgga ttatggacgg taaccagctt ggagccgtcc ggaaaggtgg cttcgacctg
     6661 gatatcgggg atcatttctg gtatgccttc catgacctgc tcgcgactca ggacatgccg
     6721 tccttcttcc atcagcgcag cgacgctttt gccgtcgcga gcgccctcca ttataaaagc
     6781 gctaatcagg gccacggatt cgggatagtt aagtttcagg ccgcgggcca gacgacgctc
     6841 tgccagcagt gcagcggtaa acagtaatag tttgtctttt tctcttggag tcagttccat
     6901 agcgttctct taagtcagcc agatccgggg aagtaccggt gatttgtcag tcagttgcgg
     6961 ccgcataaat tgccagatat cacgcattac ctgctggcaa cgcagattat catcacttaa
     7021 aaagcgaact gtcagtagac catcggtgag cgtggctccg gcgtagtttt ccagtggtgt
     7081 gagtttttcg cgtatgtctt ccagttgcgt ttcattcccc gggtagaaca gcatcgttcc
     7141 cacccatggt ttccgggcaa cgcaggtaag ttctccattg gccacgtgca gacgctcaat
     7201 caacagcgga gaaccgtcga cccatatctc cagacggtta gcgagtgttc cgtggctgaa
     7261 ggtttctccg atgaccggac gtccaaggca cagaagatcc caggccagca gtgtactgga
     7321 ggacgacaga tgaaacacgg tatgcaacgt ggcgtgagcg ccgggaaaaa aaatcgaatc
     7381 ctgtggcaac cactccagcg ttgcgttatt gcacagcgtc aggttttgct gaaggtgcgc
     7441 ctgcgcgccg ctgctgcggt aaaatttact ggcaccaggc atggtgatca gcgtatggca
     7501 atcagagtca attattgcgt tgatgatgag ttcgtcaccg ccgacgatac caccgggtgg
     7561 atgaagcaga cagaggtggc aggtttcttc ttcgggataa aaaggacgct gaacagtgag
     7621 cggaccgaca tgatgacggg tggtgagcgt ggttttcccg tgcagacgct gaaaacggag
     7681 atccagcatg gcctgccagc cttttttatt ttgtggtaac gcggtactga gcaatcttac
     7741 gcccctgata aataaagata aaacgacagt aaagagccgg atttccttgt tatataaaag
     7801 atgatgcctt tccagtgagt tttcgtctga ataatacgaa tgtttgtttt cgtcatgaag
     7861 cgttccggct cccggggcta tacatcgcca gaaaagcaat gcatattgtc gggattatcc
     7921 agagaatcag gcagaaagat ccgacaatgg gctgtgatta tgccatctgg ctcagaatga
     7981 tatcaaggtg ttatggggat aacttgctga tacaatagca gtatgacaga ttcattaatg
     8041 cggaaactgc tcactgaaac agagtgttca tgactgagca ctggtcgggt catgcagatg
     8101 ataatttccc cttttcttgg ggaagcgaca gtattttcat gcatgcctgt cttcagtcat
     8161 acggggagag catctgtgat acagtccata catttgttgc ggacgtcgct cctgagtcac
     8221 tgactggtct gcaaacagtt tatctgtaca cggcagggct tctgtgtgat atcgtttttc
     8281 atatcgaggg ggggcctcca gagatgaatg gaataataat atttcctgat gatataatac
     8341 cttagagaat ttctgtgtat gctattgtga acatattttt tattaaaaag atgttagtgg
     8401 tgttgtaaaa taaaagaatc
//
LOCUS       DAZKIV010000174         8398 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZKIV010000174 DAZKIV010000000
VERSION     DAZKIV010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.174"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..445)
                     /locus_tag="ACHOT6_004109"
                     /pseudo
     CDS             complement(1..445)
                     /locus_tag="ACHOT6_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
     gene            797..1060
                     /locus_tag="ACHOT6_004110"
     CDS             797..1060
                     /locus_tag="ACHOT6_004110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949239.1"
                     /translation="MKKPGRDYVGLVTQVLLPEQVSKEAIKLPDKTGDIVARPIEIVV
                     KCSFVLLKVIDVKKTQRTQNVFLSPQMFLAQFIHSINCGEHDR"
     gene            complement(1371..2693)
                     /gene="hipA"
                     /locus_tag="ACHOT6_004111"
     CDS             complement(1371..2693)
                     /gene="hipA"
                     /locus_tag="ACHOT6_004111"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFP9949240.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            complement(2693..2959)
                     /gene="hipB"
                     /locus_tag="ACHOT6_004112"
     CDS             complement(2693..2959)
                     /gene="hipB"
                     /locus_tag="ACHOT6_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFP9949241.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3168..8334)
                     /locus_tag="ACHOT6_004113"
                     /pseudo
     CDS             complement(3168..8334)
                     /locus_tag="ACHOT6_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
BASE COUNT         2061 a         2259 c         1788 g         2290 t
ORIGIN      
        1 catttattcc ttcttcccac agatcaggag aaacatagcc gcgagccatt ctgccaacat
       61 aaatttgcgg aacggagagt gacaaatgtt gctgaccaac atcaaactca gttgatgcgt
      121 cggccaaacg atcctgaagt ggtgtgcatg cgctgttgtc cggcacatgt ttttctgccg
      181 aatgttcttt attgtctaat aacgctattt tttttatacc aagactgaca agaagatctg
      241 tactcaggca tgggatgagt tctgcgttat tatcatctgc aataaaagta atatcccgac
      301 tagtcataaa ttcgtcgttc aaataaatgt cgacccgata tgtaccaggt ggtgcctcgc
      361 gtcccttagt aaaggcggat aaatcgacag attcagcaag atcattgctt aaaaaccgtg
      421 gattaaaata atactctgcg tcaactcctc cggcagaagc agaaaaagct tattatgctt
      481 ccatcggaaa caatgatctg gcagcctgag ttcacagata aaacactctc caggaaaccc
      541 ggggcggttc actttgctga ctggtgatac ttttgtatcg gtgtgcgggt tcgttcctag
      601 atacttcaac tctgttgaag accttaattc tgtcagcgag atctctgtgg ttcgcctggt
      661 taatctggtg agcttcaaca ggtgacatat cctggtatgc cagattcata tatctatagt
      721 tgagatactt aaggaatcgg ccaacgtttc tgacgtggtt accataggtg gacttgttgg
      781 ttctggagag tgcagaatga aaaaacccgg tagagattat gtcggtctcg tcactcaggt
      841 gctgcttccc gagcaggtaa gtaaagaagc catcaagctc cctgataaaa caggagatat
      901 tgtagccaga ccgatagaaa tcgtagtcaa atgttcgttt gtgcttctta aagtaataga
      961 cgtaaaaaaa acgcagcgaa cacagaatgt tttcttgagt ccccagatgt ttcttgctca
     1021 gttcattcat tccataaatt gtggcgaaca tgaccggtag ggtagtcaca tcatcaacta
     1081 tgagatgagt tctctggttg ttcctcgata aatgtaagat ggaatatgcc atgaacttag
     1141 cggatgcccc gtaatttttg cgttatgaag ccatcgtaag tccacaacta aactttggca
     1201 actgactgat tcatctgtaa gttcctgaaa gagaggaact catgaactta agaacaaacc
     1261 caccatattt aacataatat acattatgcg caccagtata aaccaaggaa gaatccagtc
     1321 gttggcggtc atgattgtcg tgctcattaa caacgaccaa accccatatc tcacttacta
     1381 ccgtattctc ggcttaaccg tccatgcaac ctcaacacat tggtttcaac tgccgtcacc
     1441 acgttctccg gaaaatctgt cggtaatgaa gtcttcacgt tatccagtgc tgccggaatc
     1501 attctggcaa agtcacttag gatttcatgc atctgcactt ccgggaatct cagcaccttt
     1561 gctgtcgcca aaaaatgtcg cggataaatt ttatcgattg ccgttttttt gcctttggat
     1621 gcgttaagcc ccattgccag tttgagatcg ctgatgtgta ttcccgtacc gccaaggacc
     1681 ggaaatgctg aaatgatgtc gtaaaatggc gtgagtcgat aactgccgcc agcctgaata
     1741 aatacggaga agttttttgc atggccatcc gttgcgccaa tcaaccactg gaagacctgg
     1801 aatttcataa aatcataacg atcgcgaagc gcctcgctgg accccatcaa aaacgccatg
     1861 atccgtgcga tgcctgggcc tccatctgat tcatatttca cagatgaagg taaaccgaat
     1921 gtctgacaca tatcctcctg tggcaagcga agtaaaaccg ttcgctcagt attccaacgc
     1981 ctgtcaaaac gttcgaccgc taacgcgcgc acccttcccg ctttaatgat ttctgcgtcc
     2041 ggaacattca acccaagttc tttcgccagc agcagacagt aatactcatt atcaacgctt
     2101 tggctgagat cgagcgtcgc attgggctgc ctgatttcgc caatcggtaa tttaatgatg
     2161 tgcgtcgtcg gcgttattcc tttcggaatg caccagtcat tgcctattct aagcagcgct
     2221 gtcttctcct gcgcgccagc aaccgagatg cgaaagtcat tttcttctct aatcatgccc
     2281 agagggatat ctgctttata agccgttaat acttcttcaa gcctggcttc agtaagcttt
     2341 tcccatgcca ttatcgggcg cgttatggtt tcattttcgg gtaataacgt tacggcacca
     2401 acgctgtctc gccctatttc tgacaataaa tcaaacggtt gtctggattt agcatgataa
     2461 cgtttaacga tccggtcacg tacaatcggg ctatcgggta acaggttatc gaagaagtta
     2521 aatacggcat ctgaggtgat attccccctc tgcaatggca gcgaaagtga caacggtctg
     2581 gcataacggc tggctaacca ctccggtgca tacttaaagg tgtgcgcgcc gttggctaac
     2641 ttagttaact cgcctacccg ctggttgttc atccaagtga caagtttagg cattaccact
     2701 ccagatcttg ctgttctgtt gattctggcg aggcattttt cgtgtcgcat agtgtcattg
     2761 agagttcaag cgactgtaaa atcttaaaaa atgtcgtgag cgtggtattg tcagggttgt
     2821 tttcgaaatt ggaaattgtc gcctgcttaa tgccaatttt tttcgccagc tcgctctgcg
     2881 tccagccatt ttgctggcga accagtttca ttgcattcgc taattgcgtt gggctataga
     2941 tcttctgaaa gctcatcatg tcatacgtcc acagcaaatt tatccgctta aggggatatt
     3001 gtcagtttta tcctttagtg aggataaagt caatctggaa acacatctta tccgctgtac
     3061 gggataaacg ctgcgatatc cgcgatcgcg gataaaaggg aggctttaaa gcctttgaag
     3121 tcggcaatat taagccgcat gccatctcga catgcggctt atacggttta ccagctatat
     3181 ttcactccca gcatgccctg agtatcgcta tagcctttat cacctagttg cacaccgaca
     3241 ttcccccaca ggctaaggtt gttatttact ttcgcctcaa ccccggtacg tacttcaccg
     3301 agattacgcg caccttcacg gcctacggtt tgaccattca tcttcacggc gtagactttg
     3361 ctgttgttga tccaattcgc ttcaatgtaa ggctggaact cacgctgttt accatcgtca
     3421 cgctggtgat ggctgttcag gtaggttttc accccaagtc gcgtttgcac atttccgtcg
     3481 ccttccgttt caatgcgcgt tccgtctttc cgggtatggt cggaatcttt cacacccatc
     3541 caggtgattt gcgcctgtgg ctggacgtac caggtattca gcgtcccttc gctgccgcta
     3601 aatgttcccg cttcaaaggt atacccaccc tcaacagagg ccgtcacacc gcgagaatca
     3661 tagtcgtcag cagaacggtt atcggaactg acgctgttat caaaccagtt atacagcgcc
     3721 cagctgtcaa cataagcgcc ggtcttattc gcatcgttct gataccaggt cgcgtacagc
     3781 ccagcgctgt aaccgctgat gcgcccatcc gatttataac ccacacgatt actctgagta
     3841 ttactgtgct gattagcgta acctgccatc acgccaagat gccagcgatc ctgcgcgttg
     3901 ctactccact gcgccaaatc gccgcctagc tgcaatacat agcggttagc ctgagtattt
     3961 agctgaccgt caccggccct tgaacgttcg tgccctccga catgacgcat ccacatactg
     4021 cttgccgacc cctgagaatg cagtgaatct gtatactgcg gctcacccag acggtcgtgt
     4081 aaacgatggc taaacagcga gttggctgcg gcaatgttgc tgatatagct tccggcctcc
     4141 gggcgataaa ctgatgggcc ttccggatcc acaacagggg gattattgat gggatcgggt
     4201 gtatccgctg gcgttacgcc gtcccattta ctggtcagat accagttttt ctcgtcattc
     4261 cccttccctt tagccagcgt gtagacgtaa gccccagctt cgacagttcc ggtggtcagc
     4321 gcgaagttac ctgcagaatt accgccaacc tcaatgagtt caataccgtt gaccgtttgt
     4381 gcaccgacgc cgccaatgtt atcaacccga acgcgagtgt tcccttgggt atcgcctttc
     4441 acgttcattt tgttgacttg ccatattaat ggcagagtcg gaaccgaaag caatttcccc
     4501 ccccacgata tccgtcgtac cctgatacgc cgcggaatcg ttgttaagag tcaggatccc
     4561 ggtacccagt tttgtcacat caccgctacc gctgatactg tgcgtcatcg cccagctacg
     4621 ctcagtggag atggtgagca ggccattatt gataacggaa gcatcaccca agtttttctg
     4681 ctcgttgacg ctgagcgcgc tgcctgtatc aatgttgaat tgccccacaa agcggctatt
     4741 atcgccatcc agctctacat ctgtcctggc ggttgcgctc acgataccct tcccgcttat
     4801 gctattacgc agttcgccag tcacgttttt tagcgttaac agaccgtcat tggcaatatt
     4861 tcctctgcct aacccctgag tattatcgag gctgacttcc gcatttgggc taatgctggt
     4921 taacgcattg tagcgtgcat taagcccctc gatagccagc gttccccctg taacattcag
     4981 gtttccgcca cctgtcagtt caccctgact gatcccacct ttatttaccg tcagcgcccc
     5041 ctctttgaac aaaacagtcg aacccatctg cccggtgaat gtctctaccg tctgcgtcga
     5101 tccattaaga tcgacgatgg ccgcgttgct gatgttcagt tcccgggtgt tgccaagtgc
     5161 accatccgca tcggtacgta atgtccccat ctgaacgtag gttgccccct gatagtcgtt
     5221 ctgaccgttg gaaagcgaaa cctgtcgcac cgtattgatt gccagatccc cttcaccacc
     5281 gatttttgcc gacatatcgg ccgttgcgcc ataggctccg ccatgttcgg ctaacgtcag
     5341 cttttgccca ccatggatgt tcagcgcttt cagcccatag ttaacgtaca aaccatcacc
     5401 tggtgcggtc gtgaggcgga atccataatc accctcggcg accaccgttc cgttttgcgc
     5461 aatgtgtaac gttttgtccg cttccacctc gtcgccctgt aaatcacgta acgttaatga
     5521 gccacccgac ccaataaccg tttgcgcctt caccagttga acgccgacat ggctatcatc
     5581 gtgttccagc aaattgagcg tcgtcagagg gttattcgcc atgggatcat tccacggttt
     5641 aggcacgtcg ataagcacgt cgcccgtccc gtttacctga tagttacggc ctttccaggt
     5701 gtagtcaccc gcgccgacaa ccagcgtatc gacgctgata tatccctctg caagcgtcgc
     5761 agcaggaata tccgtatcga aaatgagcgt accgccattc atggccagtc caccaatgga
     5821 ttgctctccc acttttaccg atgtggtatt ttcactgtca gactgcaaca tcgcgtgagt
     5881 aagcgcagcg gtgttgtcgc gttccagagt gaaggtactg tctttcagtt gggcaacacc
     5941 ggcgaattca gtccctgttg catgggtaaa gccaaatatc ttgtcggagg atgacagctg
     6001 cactcgcatc aaaccatcgc ctttgagcgc attggtcagc gtgtaatctc ctgcactgtt
     6061 cgttgatgcg agaatgtcga gcgtaccggg gcttgcaatg gtgacttcac tcgaacccat
     6121 gcccgttacc ccatctgccg tatgagacgc aatggtcagg aggccgtcat tgaccagcgt
     6181 tttaccgcta taagtattcc ctacattgaa caccgtatgg cctgcatcat ggatcacttt
     6241 tccgtcttta tcgtcacctg taatcagcat atcgacctgg tagccggcat cactgttatt
     6301 agtatgatta aagacaaaaa caccttcgcc agaaccaaac tctactttcg tcgcgttggt
     6361 gataaacccg gcatctgccg ccgcctcacc gtgagcagca ccaatgttta gcgttcctac
     6421 agcaggttca aaaacaccta agtaaacttc tccaccttcg acattcagcg tgccgctatt
     6481 cgtcagggtt aacgtacccg taccagatgt ccctacgtac atattgaatg tttcaagaag
     6541 agagttctgc ccatccaccc tcacgtcgcc cttacttttg tcattgagag ctatctgtgt
     6601 atcacgcgct ttaactatac ctccggtggt aatattcagt tcacccgcgc ctaatacacc
     6661 gacctgtagc aattgggcgt tagtagatga tgtctttaaa ttccagagac tatccgttga
     6721 aatattaact atccccttac cggaggcatt tcctccgata aatccatagc ccgttgaatt
     6781 aagggtggca ccattactga catttacgat accgctacca aaattaccga cattgatacc
     6841 cacagtatcg aacgatgatg ccccatccat caggttcagt gtgccgatac cgcttgcttt
     6901 atcgcccaaa aaagtaatat tttgcgatac aaacttaccg ccatcgctaa tattgaggtt
     6961 tccgacgccc gcaacaccaa tggtggtata gacattctta ttgacctccc acatcccccc
     7021 cgtggtgatg ctgacattcc cgacgccggt ttctccataa ccgagtgagc ttcctccgtt
     7081 gctgacaaca agcccctcat tactgatatt catttcgccg tggccgtcat aaccaagatt
     7141 agttccgaga ttagtgataa cggagttctt atccttaaca gtaatgttgc ctgtgcctgt
     7201 ttctttcatc cccgcagtga taattcccga atctactttg ccttcactcg agacattaag
     7261 ttcaccatcg ccagcatcac cgatatagat atagcggaaa gcttcgcccg ttccgaggaa
     7321 attccaatgc cctttatcgg tcacgttgac gacaccgtgg gaaccgtcct gaacgcccac
     7381 caatgaatat tctttgttat taatcagtcc attattggaa atattgactg tgccattgcc
     7441 gaaatatcca ttatagagtc tgcgtaccgt gatgacagag tcttgatcct gcacattcag
     7501 ggttccgaaa ccggtggcat tgccaccgat aatcgtgttt tcagccgtaa tcagcccacc
     7561 ctcgcgaata gtcgcctccc cagctccttg attaccaatt tgaaattcaa ttgaggaatc
     7621 attatttttt attagccact cgccaccctt ttcaacgata accttaccat tactgttcgc
     7681 ttgatatcct aaaatggcga ctattgaact cgtgacgtaa cccttatccg taatatttaa
     7741 tgagcccgta ccgtaactcc ctatttcgaa taattcggtc gtcagaacag agtcttctcc
     7801 ctcaacattg accgtcccga cgcctcctgt cgaggaacct agtcttaaat agcctccatc
     7861 aacgtgacct ttctgtttaa tattcagcgt ccccgttccg gattgaccca catttaaagg
     7921 ccttgcatta tttccgctat catacaatcg ccaggtgcca cccaaaacat taacggtacc
     7981 ctccgaatcc tcattagcgc caatgacgct ggtagtaata gtggttaagg aggcattacc
     8041 gccagccaaa atattaagca ctccagtgcc ataccagcca accaggtagg catcataagc
     8101 aacatcagta tcaatattgg taatctgatc attatcaact tccagacttg caccagacgc
     8161 tgaaccgctt agtgccagca aaacacccag cgtcaatcta ctgaatttcg ttgtcagtcc
     8221 agaggattta cgcaaattaa ccgtcgatgt tttacctgcc ctgcgagtta attccgagca
     8281 ggcctgaaat acctgtagag tgcaattcca tatcacgcga tagattctat tcatataaac
     8341 gctccatata caaacaatac agccacgaaa gagatgagtc gtcagacatt aaaaatga
//
LOCUS       DAZKIV010000175         8340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZKIV010000175 DAZKIV010000000
VERSION     DAZKIV010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.175"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(77..283)
                     /locus_tag="ACHOT6_004114"
     CDS             complement(77..283)
                     /locus_tag="ACHOT6_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFP9949242.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            596..685
                     /gene="kdpF"
                     /locus_tag="ACHOT6_004115"
     CDS             596..685
                     /gene="kdpF"
                     /locus_tag="ACHOT6_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFP9949243.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            685..2358
                     /gene="kdpA"
                     /locus_tag="ACHOT6_004116"
     CDS             685..2358
                     /gene="kdpA"
                     /locus_tag="ACHOT6_004116"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFP9949244.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVMTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLTPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2381..4429
                     /gene="kdpB"
                     /locus_tag="ACHOT6_004117"
     CDS             2381..4429
                     /gene="kdpB"
                     /locus_tag="ACHOT6_004117"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFP9949245.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASAFIPTQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4438..5010
                     /gene="kdpC"
                     /locus_tag="ACHOT6_004118"
     CDS             4438..5010
                     /gene="kdpC"
                     /locus_tag="ACHOT6_004118"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFP9949246.1"
                     /translation="MSGLRPALSTFIFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASVPVELVTASASGLDNNITPQAAAWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5003..7687
                     /gene="kdpD"
                     /locus_tag="ACHOT6_004119"
     CDS             5003..7687
                     /gene="kdpD"
                     /locus_tag="ACHOT6_004119"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFP9949247.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEDENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7684..>8340
                     /gene="kdpE"
                     /locus_tag="ACHOT6_004120"
     CDS             7684..>8340
                     /gene="kdpE"
                     /locus_tag="ACHOT6_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFP9949248.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     I"
BASE COUNT         1731 a         2186 c         2431 g         1992 t
ORIGIN      
        1 cctctgaaca tcttcctgat acaaaaaacc gccagcgttt ccgctggcgg ttttcaatgt
       61 attcaaaact cagcaatcaa taaaaatcac cagttgcctt ttcggcctga tccatccaca
      121 ccggtttatc gctggttttc gaccagacgc gatgcaggta gctgtaaaag cgggcgcggt
      181 ctttccagaa aagcatgaaa ggcagcgcca gaacacccgc tgcaaccgca taagtacggc
      241 gtaaaaagat aagccatgca ggatattctc tgtagagttc catacttttc tccccctttt
      301 gtatctaccc ggtgaatggc accggaaaaa tgaatttgtt tatctgatga aaatagtacc
      361 gccttttgtg taattttact actcatccga ccacttattt ttgcttattg atggtttatt
      421 tacattcatc ctgtaattaa gttacacaaa agttaaatta atactaaaca ttagttaaat
      481 catggctttt gccattttta tacttttttt acaccccgcc cgcagatttt tgcgaaatct
      541 ttgcagccag aattctaccc ttccggtatc acttttaggc cactggaggt gcactgtgag
      601 tgcaggcgtg ataaccggcg tattgctggt gtttttatta ctgggttatc tggtttatgc
      661 cctgatcaat gcggaggcgt tctgatggct gcgcaagggt tcttactgat cgccacgttt
      721 ttactggtgt taatggtgct ggcgcgtcct ttaggcagcg ggctggcgcg gctgattaat
      781 gacattcctc ttcccggtac aacgggcgtt gagcgcgtac tttttcgcgc acttggcgtc
      841 tctgaccgtg agatgaactg gaagcaatat ctctgtgcca ttctcggcct gaacatgctg
      901 gggctggcgg tgctgttttt tatgttgctc ggtcagcact atctgccgct taatccacag
      961 cagttgccgg ggctgtcgtg ggatctggcg ctgaataccg ccgtcagctt tgtcacgaat
     1021 accaactggc aatcttacag cggtgaaacc acgttgagct atttcagcca gatggctggc
     1081 ttaacggtgc aaaactttct ttctgccgcc agcgggattg cggtgatttt tgccctcatc
     1141 cgtgcgttta cccgccagag catgagcaca ctcgggaatg cctgggtcga tctgctacgc
     1201 atcacgttat gggtgctcgt ccctgtggcg ttgttgatcg cactattttt tattcaacaa
     1261 ggtgcgctgc aaaactttca gccttatcag gctgtgaata ccgttgaagg agcgcaacag
     1321 ctgttaccca tggggcctgt agcttctcag gaagcgatca agatgctcgg tactaacggc
     1381 ggtggcttct ttaatgccaa ctcgtcgcat ccgtttgaaa acccaaccgc actgaccaac
     1441 ttcgtgcaga tgctggcgat cttcttgatc ccaacggcgc tgtgctttgc ctttggtgaa
     1501 gtgacgggcg atcgccgcca ggggcgcatg ttgctgtggg cgatgtcagt gatttttgtc
     1561 atctgcgtag gcgtggtgat gtgggcagaa gttcagggta atcctcatct gctggcgctc
     1621 ggcgcagaca gcagcatcaa tatggaaggt aaagagagcc gtttcggcgt gctggtcagt
     1681 agcctgtttg cggtcatgac tacggcggct tcctgtggcg cggtgattgc gatgcatgat
     1741 tcgtttaccg ctctcggtgg catggtgccg atgtggctga tgcaaattgg tgaagtggtg
     1801 ttcggcggtg tcggttctgg tctttacggc atgatgctgt ttgtcctgct ggcggtgttt
     1861 attgccgggc tgatgattgg tcgtacaccg gaatatctgg gtaaaaaaat cgacgtacgc
     1921 gagatgaaac tgactgcact ggcgattctg gttaccccga cgctggtgct gatgggcgca
     1981 gcgctggcaa tgatgaccga cgccggacgt agcgccatgc tcacccctgg cccgcatggt
     2041 tttagcgaag tgctgtacgc cgtgtcatcc gccgctaaca acaacggcag cgcctttgcc
     2101 ggattaagcg ccaactcccc gttctggaac tgtttactgg cgttctgcat gtttgtcggt
     2161 cgcttcgggg tgattatccc ggtgatggca attgccggtt cgctggtgag taaaaagagc
     2221 caacccgcca gctccggcac gctgccaacg cacggcccgc tgtttgttgg cctgttaatc
     2281 ggcaccgtgt tgctggttgg cgcactgacc tttatccctg ccctggcgct tggtccggtg
     2341 gcggaatatc tctcctgatg atattgagtg agcactgaat atgagtcgta aacaactggc
     2401 gctattcgaa ccaacacttg tcgttcaggc gctgaaagaa gcggtgaaaa aattaaaccc
     2461 gcaggcgcaa tggcgcaatc cggtgatgtt tatcgtctgg atcggcagtc tgctgaccac
     2521 ctgtattagc atcgctatgg caagcggtgc gatgcctggc aatgcgctgt ttagcgcggc
     2581 aattagcggc tggctgtggg tcaccgtact gttcgctaat ttcgccgagg cgctggcaga
     2641 aggccgcagt aaagcgcagg ccaacagtct gaaaggggtg aaaaaaactg cctttgcccg
     2701 caagctgcgt gagccgaaat atggcgctgc ggcggacaaa gttcctgccg atcaactgcg
     2761 taaaggtgac atcgtgctgg tagaagctgg cgatattatc ccctgcgatg gtgaagtcat
     2821 tgaaggtggc gcatcggtcg atgaaagcgc catcaccggg gaatcggcac cggtgatccg
     2881 tgaatccggc ggcgattttg cctccgtcac cggcggtacg cgtattcttt ctgactggct
     2941 ggtgattgag tgtagcgtta accccggaga gacatttctg gatcggatga tcgcgatggt
     3001 ggaaggcgcg cagcgacgta aaacgccgaa cgagattgcc ctgaccatcc tgctgattgc
     3061 cctgactatc gtctttttac tggcaaccgc cacgctgtgg ccgttttccg cgtggggcgg
     3121 taatgcagtc agcgtaacgg tactggtggc gctgctggtc tgtctgatcc caaccactat
     3181 tggcgggctg ttgtcagcga tcggcgtcgc cgggatgagt cggatgctgg gcgcaaatgt
     3241 gattgccacc agcggacgtg cagttgaagc ggcaggtgac gttgacgttc tgcttctgga
     3301 taaaaccggc accatcacac tcggtaaccg tcaggcgtcg gcgtttatcc ccacgcaggg
     3361 cgtggatgaa aaaacgctgg ctgacgccgc acaactggct tcgctggctg atgaaacgcc
     3421 ggaaggccgc agtattgtga tcctcgccaa gcaacgtttt aacctgcgcg agcgcgatgt
     3481 gcagtcgctc catgccacct ttgtaccgtt tactgcgcaa agccggatga gcgggatcaa
     3541 catcgacaac cgcatgatcc gtaaaggttc tgtcgatgcc attcgccgcc atgttgaggc
     3601 taacggcggt cacttccctg ccgatgttga tcaaaaagtc gatcaggttg cgcgtcaggg
     3661 agccacgccg ctggtggtgg tggaaggttc tcgtgtgctg ggcgttattg cgctgaaaga
     3721 tatcgtcaaa ggcggtatta aagagcgctt cgcccagctg cgcaaaatgg gcattaaaac
     3781 ggtgatgatt accggcgata accgtctgac tgccgccgcc attgctgcgg aagcgggcgt
     3841 cgatgatttt ctcgccgaag cgacaccgga ggccaagctg gcattgattc gtcagtatca
     3901 ggcggaaggc cgtttggtgg cgatgaccgg cgacggcacc aatgatgctc cggcgctggc
     3961 gcaggcagat gtcgcggtag cgatgaactc cggtactcag gcggcgaaag aagcgggcaa
     4021 tatggtcgat ctcgactcta acccgaccaa gttgattgag gtggtgcata ttggcaaaca
     4081 gatgctgatg acccgtggct cgctgaccac cttcagtatt gccaacgatg tggcgaaata
     4141 cttcgccatt attccggcgg cgttcgcggc aacgtatccg cagttaaatg cgctgaacat
     4201 catgcgcctg cattcgcccg actccgccat tctcagtgcg gtcattttca acgccttgat
     4261 tatcgtcttt ttgattcctc tggcattaaa aggcgtgagt tataaaccgc ttaccgcttc
     4321 tgccatgttg cgccgtaact tatggattta cggtctgggt gggctgctgg tgccgtttat
     4381 tggtatcaaa gtcattgatt tactgctgac cgtttgcggt ctggtgtgag gtttaccatg
     4441 agtggattac gtccggcatt atcgacattt atctttctgt tattgattac tggcggtgtt
     4501 tacccgctgc tgaccaccgc gctggggcaa tggtggtttc cctggcaggc caacggttcg
     4561 ttgatccgtg aaggtgatac agtgcggggt tcggcattaa tcgggcagaa ttttactggc
     4621 aacggctatt ttcatggtcg cccgtcggca acggcagaaa tgccctataa tccacaggct
     4681 tctggcggga gcaatctggc ggtcagtaac cctgagctgg ataaactaat agccgcacgc
     4741 gttgctgcat tacgggccgc taacccggat gccagcgcga gcgttccggt tgaactggtg
     4801 acggcatcgg caagcgggct ggacaataat atcaccccgc aagcggcggc ctggcaaatc
     4861 ccacgcgtgg cgaaagcgcg taatctcagc gttgaacagc tcacgcaact gatcgcaaaa
     4921 tacagccaac aaccgctggt gaaatatatc ggccagccgg tcgtcaacat tgtcgaactc
     4981 aatctggcac tggataagct tgatgaataa cgaaccctta cgtcccgacc ccgatcgtct
     5041 gctggaacaa actgccgcgc cgcatcgggg gaagctgaaa gttttcttcg gtgcctgtgc
     5101 aggcgtcggg aagacctggg cgatgctggc agaagcccag cgactgcggg cgcaagggct
     5161 ggatattgtg gttggcgtgg tagaaaccca cggacggaaa gataccgccg ccatgctgga
     5221 agggctggct gttctgccgc caaaacgcct ggcgtaccgt gggcggcata tcagcgagtt
     5281 tgatctcgat gccgccctcg cccgccgtcc ggcgctgatc ttaatggacg aactggcgca
     5341 cagtaatgcg ccaggttctc gtcatcctaa acgctggcag gatatcgaag aactgctgga
     5401 agctggcatt gatgttttca caaccgtcaa cgttcagcat ctggaaagtc tgaatgatgt
     5461 ggtcagcggc gtcaccggaa ttcaggtacg ggaaaccgtg cccgatcctt ttttcgatgc
     5521 cgccgacgac gtagtgctgg tggacttgcc cccggacgat ctgcgccagc ggctgaaaga
     5581 aggcaaagtc tatattgccg ggcaggcgga gcgcgccatt gaacattttt tccgcaaagg
     5641 caatctgatc gccctgcgtg aactggcact gcgccgtact gccgatcgcg tagatgagca
     5701 aatgcgcgcc tggcgggggc atcctggcga agagaaagtg tggcacacgc gcgacgcgat
     5761 ccttttatgc atcgggcata acaccggcag tgagaaactg gtccgcgcgg cggcgcggtt
     5821 ggcctcgcgc ctgggtagcg tctggcacgc ggtgtatgtt gaaacccctg ccctgcatcg
     5881 cttaccggaa aaaaaacgtc gggcaattct cagcgcctta cgtctggcgc aggaactggg
     5941 cgcggagacg gcaacacttt ctgatccagc ggaagagaaa gcggtagtgc gttatgcccg
     6001 tgaacataat ctcggcaaga ttattctcgg tcgcccagcc tcgcgccgct ggtggcgtcg
     6061 ggaaacgttt gctgaccgac tggcgcgcat cgcccccgat ctcgatcagg tgctggtcgc
     6121 gcttgatgaa ccacccgccc gcacgattaa caacgcgccg gatagccgct cttttaaaga
     6181 caagtggcgg gtacagattc agggatgcgt ggttgccgcc gcgttatgcg ccgttatcac
     6241 cttaattgcc atgcagtggc tgatggcgtt tgatgccgcc aacctggtga tgctgtatct
     6301 acttggcgtg gtggtggtgg cgctatttta tggacgctgg ccttctgtgg ttgctaccgt
     6361 cattaatgtg gtgagtttcg atctcttttt tatcgcccca cgcggcacgc tcgccgtctc
     6421 tgatgtgcaa tatctgctga ccttcgcggt gatgttaacc gtcgggctgg tgatcgggaa
     6481 ccttactgct ggcgtgcgtt atcaggcgcg ggtagcccgt taccgcgagc aacgcacacg
     6541 gcacttatat gaaatgtcga aagctctggc ggtgggccgc agtccgcagg acatcgccgc
     6601 caccagcgaa caatttattg cctccacgtt tcatgcccgc agtcaggtgt tgttgcccga
     6661 tgacaacggt aaattgcagc cgttaacaca tccgcaagga atgacgccgt gggacgatgc
     6721 catcgcgcag tggagttttg ataaaggcct gcctgcgggc gcgggcaccg acacattacc
     6781 cggcgtgccg taccaaattt tgccgttaaa aagcggtgag aaaacctacg ggctggtggt
     6841 ggtggagccg gggaatctgc gccagttgat gatcccggaa caacagcgcc tgctggagac
     6901 atttacgttg ttagtcgcca atgcccttga gcggctgaca ctgaccgcca gcgaagaaca
     6961 ggcgcggatg gcaagcgaac gtgaacagat ccgcaacgct ctgctggcgg cgctttcgca
     7021 tgatttacgc acgccgctca cggtgctgtt cggtcaggca gaaatcttaa cgctcgatct
     7081 ggcaagcgaa ggctcgcccc acgcccgcca ggccagcgag attcgtcagc atgtgctgaa
     7141 cactacccga ctggtgaata atctactgga tatggcgcga attcagtccg gcggctttaa
     7201 tttgaagaaa gagtggttaa cgctggaaga agtagtcggc agcgcgctgc aaatgctgga
     7261 accgggttta tcgtcgccca tcaatctttc tctgccagaa ccgctgacct taatccacgt
     7321 tgacgggcca ctctttgaac gggtgctgat taatctgctg gagaacgcgg tgaaatatgc
     7381 gggtgcgcag gccgaaattg gtatcgatgc ccacgttgag gacgaaaatc tacaactgga
     7441 tgtctgggat aacggccccg gtcttccgcc aggccaggag cagacgatat ttgataagtt
     7501 tgctcgcggg aataaagagt cggcagtacc gggtgtaggg cttggactgg caatttgtcg
     7561 ggcgattgtg gatgtacacg gcggcaccat taccgcgttc aaccgaccgg aaggtggtgc
     7621 ctgttttcgt gttacacttc cccagcaaac cgctcctgaa cttgaagact ttcatgagga
     7681 tatgtgacaa acgttctgat tgttgaagat gaacaggcta ttcgtcgctt tctgcgcacg
     7741 gcgctggagg gcgacgggat gcgcgtctat gaggccgaaa cgctgcaacg cggcttgctg
     7801 gaagcggcta cccgtaagcc agatttgatt attctcgatc tcggcctgcc cgatggtgat
     7861 gggattgagt ttatccgcga cctgcgccag tggagcgcgg tgccggtgat tgtgctttcc
     7921 gcacgcagcg aagagagcga caaaatcgcc gcgctggatg ccggagcgga tgattatctg
     7981 agtaagccgt ttggcattgg cgaattgcag gcccgtctgc gcgtcgcttt acgccgccac
     8041 tccgccaccg ccgcgcccga tccgctggtg aaattttccg atgttaccgt cgatttagcc
     8101 gcccgcgtga ttcaccgggg tgaggaagag gtgcatctca cgccgattga gttccgcctg
     8161 ctggcggtgc tgctgaataa tgccgggaaa gtgctcaccc agcgccagtt actcaatcag
     8221 gtgtgggggc caaacgcggt cgaacacagt cactatttgc gtatttatat gggacatctg
     8281 cgacaaaaac tggaacagga tcccgctcgc ccccgccatt tcattactga aaccggtatt
//
LOCUS       DAZKIV010000176         8227 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZKIV010000176 DAZKIV010000000
VERSION     DAZKIV010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8227
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.176"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(78..1316)
                     /locus_tag="ACHOT6_004121"
     CDS             complement(78..1316)
                     /locus_tag="ACHOT6_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFP9949249.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            1652..2854
                     /gene="nupC"
                     /locus_tag="ACHOT6_004122"
     CDS             1652..2854
                     /gene="nupC"
                     /locus_tag="ACHOT6_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFP9949250.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(2904..5093)
                     /locus_tag="ACHOT6_004123"
     CDS             complement(2904..5093)
                     /locus_tag="ACHOT6_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9949251.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEVGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(5301..5376)
                     /locus_tag="ACHOT6_004124"
     tRNA            complement(5301..5376)
                     /locus_tag="ACHOT6_004124"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5341..5343),aa:Ala,seq:ggc)
     gene            complement(5416..5491)
                     /locus_tag="ACHOT6_004125"
     tRNA            complement(5416..5491)
                     /locus_tag="ACHOT6_004125"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5456..5458),aa:Ala,seq:ggc)
     gene            5712..6071
                     /locus_tag="ACHOT6_004126"
     CDS             5712..6071
                     /locus_tag="ACHOT6_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9949252.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            6073..6465
                     /locus_tag="ACHOT6_004127"
     CDS             6073..6465
                     /locus_tag="ACHOT6_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFP9949253.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(6517..7932)
                     /gene="gltX"
                     /locus_tag="ACHOT6_004128"
     CDS             complement(6517..7932)
                     /gene="gltX"
                     /locus_tag="ACHOT6_004128"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFP9949254.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(8045..>8227)
                     /locus_tag="ACHOT6_004129"
     CDS             complement(8045..>8227)
                     /locus_tag="ACHOT6_004129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949255.1"
                     /translation="CKGGALPAELITPAVWSRIIGRVQNESTHFLKKLFVRRKFKQDD
                     RKTDRVAQFVNENNNA"
BASE COUNT         2189 a         2142 c         1925 g         1971 t
ORIGIN      
        1 ccggctcttt aacgcgtagg gcggataagg cgtttacgcc gcatccgcca atagtgccag
       61 atgcgacgct cattcaacta caatcccagc gccgtcccca ccaacaacca gatattcagc
      121 gccacgacca gcactacaat cacccagcct gtctgtttta cgcgtttgct gttcaccaga
      181 tcgcccatca acttgctgtc actggtgaaa atcagcagtg gaaccagcgc cagggcgata
      241 ccaaaactta acagcacctg actcataacc agaatccgtg tcggatctaa tcccatcaga
      301 atgacaataa atgacggcaa catggtgact gtacgacgca cccacagcgg gatatggaag
      361 cgaatgaatc cctgcatcac cacctgcccc gccagtgtcc ccaccaccgt tgaggacagt
      421 ccggcagcaa ccagacttaa cccaaagacc gttgccgcag catggcttaa cagcggttgc
      481 agcgtcagat aagcctcatc aagatcggca acaccagtat gaccagaaaa gtggaacgcc
      541 gccgcagctg tagccatcat cgccagattg acaaaaccgg caatcgtcat ggcgatagcc
      601 acatcccatt tggtggcgga ataacgttgt tgacgcgaac cgccatgtaa atgctgagtg
      661 agcgaagagt gcaaataaat cacatgcggc ataatcgtcg cccctaacac gcctgctgcc
      721 aggaagaccg cctccgaagt aggtaaactc gggatcacca ttcctttacc cagctgcgcc
      781 aggttaggct gggagaaaat caactcgaca atgtaagccg cggcaacaaa caacagtaac
      841 ccgccaatca ctttctccag cggtttttgc ccgcgacgtt gcagcattaa aatcaggaaa
      901 gtcgcgatcc ccgtcagcac cgcgccctgc aacaacgaaa caccaagaat gagtttaaaa
      961 ccgatcgccg caccaataaa ttccgccagg tcggttgcca tcgcaataat ttctgcctga
     1021 acccaataga accacactac gggacgcgga tagtgatcac gaatctgctc cgccagattt
     1081 ttaccggtgg caatccctag tttggcagag aggatctgaa tcagcatcgc catcaggttg
     1141 gcccaaacga caacccacag tagctgatag ccgaagctag cacccgcctg aatattggtc
     1201 gcaaagttac cgggatcgat ataaccaatc gccgcaatga acgcaggtcc cattaatgcg
     1261 agcctcatct tgcgcgccgc ccgtccgctg ctactctcaa cgcgatagtt cgtcatcttg
     1321 tgcctctaaa acatagcctt tgctatgttt catgctatgc caaacgagaa tgattatcaa
     1381 attcatttga atgggttgtg gtgatttctt tgatagacca ggattatgac tacgaaaaga
     1441 ttgatgactt gaatgttatg gttatgttta atgttagcac atttacataa ctttcagctt
     1501 ccatacacaa catagcagaa atgtatgaca gatcactatt tttgaagctt gtcacaggac
     1561 gtcattatag tgtgtgtcag atctcgtttt ccttaaccat gttacataga atgtgcacgg
     1621 aaatttaacc tgcctcatat ttggagcaaa tatggaccgc gtccttcatt ttgtactggc
     1681 acttgccgtt gttgcgattc tcgcactgct ggtaagcagc gaccgcaaaa aaattcgtat
     1741 ccgttatgtt attcaactgc ttgttatcga agtgttactg gcgtggttct tcctgaactc
     1801 cgacgttggt ttaggcttcg tgaaaggctt ctccgaaatg ttcgaaaaac tgctcggatt
     1861 tgccaacgaa gggactaact tcgtctttgg tagcatgaat gatcaaggcc tggcattctt
     1921 cttcctgaaa gtgctgtgcc caatcgtctt tatctctgca ctgatcggta ttctccagca
     1981 cattcgcgtg ttgccggtta ttatccgcgc aattggtttc ctgctctcca aagtcaacgg
     2041 tatgggcaaa ctggaatcct ttaacgccgt cagctccctg attctgggtc agtctgaaaa
     2101 ctttattgcc tataaagata tcctcggcaa aatctcccgt aatcgtatgt acaccatggc
     2161 tgccacggca atgtccaccg tgtcgatgtc catcgttggt gcatacatga ccatgctgga
     2221 accgaaatac gtcgttgctg cgctggtact gaacatgttc agcaccttta tcgtgctgtc
     2281 gctgatcaat ccttaccgtg ttgatgccag tgaagaaaac atccagatgt ccaacctgca
     2341 cgaaggtcag agcttcttcg aaatgctggg tgaatacatt ctggcaggtt tcaaagttgc
     2401 cattatcgtt gccgcgatgc tgatcggctt tatcgccctg atcgccgcgc tgaacgcact
     2461 gtttgccacc gttactggct ggtttggcta cagcatctcc ttccagggca tcctgggcta
     2521 catcttctat ccgattgcat gggtgatggg tgttccttcc agtgaagcac tgcaagtggg
     2581 cagtatcatg gcgaccaaac tggtttccaa cgagttcgtt gcgatgatgg atctgcagaa
     2641 aattgcttcc acgctctctc cgcgtgctga aggcatcatc tctgtgttcc tggtttcctt
     2701 cgctaacttc tcttcaatcg ggattatcgc aggtgcagtt aaaggcctga atgaagagca
     2761 aggtaacgtg gtttctcgct tcggtctgaa actggtttac ggctctaccc tggtgagtgt
     2821 gctgtctgcg tcaatcgcag cactggtgct gtaaaaccat acataaaaaa gccggggatc
     2881 attccccggc ttttttacgc ctgttaatca gctaatggct gcgggcgacc aatcaaatac
     2941 ccttgcagat attgcacccc gagcttatgc aatagcgcct gctgctgttg cgtctcgaca
     3001 aactccgcga ccacactcaa tgacttcgct ttcgccagat cggtaattga tctcacaatc
     3061 atcgcatcca gcgtattcgt gacaatatct ttcacaaaga cgccatcaat tttgatgata
     3121 tcagcctgca aacgctttaa ccgttcgtag ttggcatatc cggtgccaaa gtcatcaatc
     3181 gcaatccgga aaccaaactt atgcagctgc tcgatgttgt acatgctgct ttctgcgtta
     3241 gaaaacgcct gctcctcggt gatctcaaga atgaccgcct gcggggagat gtgataacgc
     3301 ttaaacagac gaataatccg cccggcaata ttcttttgca gcagcgtgag cggcattaaa
     3361 ttgactgaaa agcgcggtcc ttttttgtcg caagggtgtg ttgccagcca cttcaacaag
     3421 gattccagca cttgcaaatc aaaacgcgcg ctaaggttaa actgagcaat aaggggcaga
     3481 aacttatccg gggtcataat gccgccgtca tatttcagtc gcgcgaggat ctcatcataa
     3541 ccttcaccct ctttgttgcg aattggctgg gcgtagagca gcaaatcacc ctgatctaac
     3601 gccgtgcgaa tggtattcag caatagcacc tgtttagtgg tctgcccgga aaccatctcc
     3661 tctctgctat ccagcgccag cacatgatga tgtgcgcagg attgctccgc cagccagctt
     3721 aactgcccca acaagggttg cagggtttcc tgatttccat caaaacgccc ccaggcagca
     3781 ccatagccca tatccagccc ggtattgttc cagtgaattt gccgactatt caggatgtta
     3841 accatatgct ggagtcgccc ttccgtttcc ggcccgctca gcactaacag cagttcacta
     3901 cccggcaatt gatacaactt ttcgttttcc tgcatcaacg gcagcagcgt acggcaaatt
     3961 gagcggatac agtgaacgcg catcattaag ccgtaatgac gactcataaa ctcaagatta
     4021 tcaatgcgca ggcagcaaaa actcttgccc acctcttgct ccggcgcttg ttccaacgca
     4081 cgaaagttgg gtaggagcgt taacggatct gtcagcgcct gcaaatgcca gcggcgatta
     4141 agccattcac tgcgatgata aatgcgcacc atatagagca ggcaaacgct aaaggaaatc
     4201 agcaccgcaa gaataaatgc cagcgaatat tcggtttcca ccccttgcaa aaagttctgg
     4261 ttgtaattca gaaggcaaag cgttgaaaca gcccaggtga gatttaaaaa cggatagcga
     4321 agcttaccga ccccgagggt aaagatgatg aagaaaacgg gtaccaagta accggcaata
     4381 aagtcgtttt cataaggtgt gcacaacaga agtagcagca cgcttaacgc tgccagccag
     4441 cttaaggtaa atgcgcgttt ctctttgccc aacgacggag cgatatccct gcgccacaat
     4501 atctgcgcaa agtggggact tacaatcatg cgagtgagat agtagaaaag catgttgtaa
     4561 atcagcacag cggtgaaaag gcttagcaaa tcaacgaccg tgaaaatcgc atccgcatcg
     4621 ccgaaaaagg tggatatctt tagcgggaaa tcaaagaagc tccccacaag atacatgctg
     4681 catttgatgc cgatcggcgt caccaaacca agccagaaca gacgttgcca gacatatcgg
     4741 ctggtcaatc cataacgcca acgcgtcccc agctgccagc gtaatattgc gcaggcacac
     4801 aacacgacaa acgtctggct accgagcatg accgcagtct gtaaaaaaga gagatgaaag
     4861 ttccacagat tggtgcaaaa catcccgcat aaaacgggaa cgacaccgcg ccagccaaag
     4921 ataaacaaga ttgacagcat tacgcacagt ggcatccacg ccagaaagat ataactggaa
     4981 tggataatgg caagtggcga aataaaacgg gatagctgaa taagtaccac ggtgagcgta
     5041 aacgctagtg tgaatatctt gatatttttt atcaggttat gctccacaaa catgagtaca
     5101 ctactctcac tgatcggggt tttcaggcac acaaataata agtttattcg catcgcgaag
     5161 caaacagggg ccagcctgtt gccgattttt catccaatga tgctttcacc aggcggcgct
     5221 aacccggacg tagactgtat tacaaaagcg gcaaaaagca gagacaaaaa acccccgctt
     5281 tgcagcgagg gttggaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     5341 gccatgcaag cgctctccca gctgagctat agccccacga tgcgtttacg taccaaattt
     5401 gctgggtgca aaatttggtg gagctaagcg ggatcgaacc gctgacctct tgcatgccat
     5461 gcaagcgctc tcccagctga gctatagccc cgtcacgtaa agcttgtcga gttgacgggc
     5521 ggcatcatat gaattccgcc cgaatgtgtc aacggcaaat tgcaacgtgt agtttcaatc
     5581 gctgaaaaat caggcaaatg aacaattttg gaaaaccgct cgcattcagt agttattcat
     5641 gtcacggttt cctgtaaagt ggtgttataa aatgaactac taatagaccc acatacattc
     5701 agggaattgt tatgttcaag gagaggatga caccagatga acttgccagg ctgaccggct
     5761 atagccgcca gaccattaat aaatgggtac gcaaggaagg ctggacaacg tcaccaaaac
     5821 ctggcgtcca gggtggcaaa gcccgactgg ttcacgtcaa tgaacaggtt cgtgaatata
     5881 ttcgcaatgc tgaacgtcca gaaggtcagg gagaagcgcc tgccctttct ggtgatgcgc
     5941 cgcttgaagt attgctggtg acactggcga aagagatgac gccagttgaa caaaaacagt
     6001 ttacatcctt gcttctgcgg gaagggatta tcggattgtt acaacgctta gggattcgcg
     6061 atagcaaata atatgaaaag attacgcaac aaaatgacca ccgaagaact ggctgaatgc
     6121 ctcggtgtgg ctaaacaaac tgttaaccgt tggatcagag aaaaaggctg gaaaacggaa
     6181 aaatttcccg gcgtgaaagg cggtcgtgcc agacttattc tggtcgatac gcaagtttgc
     6241 gagtttattc agaacacgcc agccttccat aacacaccaa tgttgatgga agctgaagag
     6301 agaattgctg aatacgctcc tggagcacgc gccccggcgt atcgacaaat catcaacgcg
     6361 atagataata tgacagacat tgagcaggaa aaagtcgcgc aatttttgtc acgtgaagga
     6421 attcgcaatt tcctcgcccg tctcgatatt gacgaatcag cataaataaa aaacggcagg
     6481 atattatctc ctgccgttta tctttttaca cgctaattac tgctgatttt cgcgttcagc
     6541 aataaaatcc agcgctttgt tgatacgctc gatactgcgg gtcttaccaa ttgcgtgaac
     6601 ggtaacatcc agtgctggag actgccccgc accggttacg gcgacacgca gcggcatacc
     6661 aactttaccc atacccactt ccagctcatc cgccgtcgcc tgaatagcgt gatgaacgtt
     6721 ttcagcggtc cagtcagtaa tcgcggccag tttgtcacga accacttcca gcggctgacg
     6781 cgctaccgga cgcagatgtt ttttcgccgc atcggcatcg aactcagcaa aatcttcgta
     6841 gaaataacgg cagctctgtg ccatctcttt cagcgtcttg cagcgttcgc ccagcagttt
     6901 caccagatca gccagctgcg ggccgttacg ggtatcgata ttttcctgct caatgtgcca
     6961 ctgtaagtga gtagcaacat actccggcgg cagcgcgtta atgtagtgat ggttcagcca
     7021 cagcagcttg tcggtgttga acgcactggc agatttgctg acggcattca aagtgaagta
     7081 tttgatcatc tcttcacgag tgaagatttc ctgatcgccg tgggaccagc ccagacgcac
     7141 cagatagttc agcagtgctt ctggcaaata accgtcatca cgatactgca ttacgctgac
     7201 tgccccgtga cgtttggaca gttttttacc gtcatcgcca ttgatcatag aaacgtgcgc
     7261 gtaaaccggc accggcgctt tcagggcctt aagaatgttg atctggcgcg gcgtgttgtt
     7321 gatatggtct tcgccacgga taacgtgggt gatttccata tcccagtcat cgacaaccac
     7381 acagaagtta taggttgggg aaccatcggt acggcggata ataagatcgt ccagttcctg
     7441 gttgctgaac tcgatcggac cacggatctg atcgtcaaaa acaacagaac cttcctgcgg
     7501 gttagcaaaa cgtacaacac acggttcatc atcagcatga tgctcatggc tgtggcggca
     7561 gcgaccgtca taacgcggct tctcaccttt cgccatttgc tcttcgcgca gcgcttccag
     7621 gcgctcttta gagcaatagc atttataagc agtgccctct tccagcatct gatcgatcac
     7681 cgcgttgtag cgatcaaaac gtttggtctg gtagtacgga ccttcatccc actccaggct
     7741 cagccagttc atgccatcca taatggcttc gatagcttcc ggcgtggaac gctcaagatc
     7801 ggtgtcttca atacgcagca cgaactcacc gccgtggtta cgtgcaaaaa gccaggagta
     7861 aagagcagta cgcgcgccgc caacgtgcag atagcctgtt gggcttggcg cgaagcgagt
     7921 tttgattttc atgaaatggc cttacgttta gaaagatgcc gacaaccggc aaatcctgga
     7981 aaaattgagt gggcgatatt ctatcactcc cgcctgattc ctcaatgtag ttcgggtttc
     8041 taccttacgc attattgttt tcgttgacaa attgcgcaac acggtctgtt ttgcgatcat
     8101 cttgcttaaa tttacgacga acgaacaatt tctttagaaa atgcgttgac tcattttgaa
     8161 ctctccctat aatgcgactc cacacagcgg gggtgattag ctcagctggg agagcacctc
     8221 ccttaca
//
LOCUS       DAZKIV010000177         7871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZKIV010000177 DAZKIV010000000
VERSION     DAZKIV010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.177"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            221..763
                     /gene="yfaZ"
                     /locus_tag="ACHOT6_004130"
     CDS             221..763
                     /gene="yfaZ"
                     /locus_tag="ACHOT6_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFP9949256.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            863..2065
                     /gene="yfaY"
                     /locus_tag="ACHOT6_004131"
     CDS             863..2065
                     /gene="yfaY"
                     /locus_tag="ACHOT6_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFP9949257.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2129..2590
                     /locus_tag="ACHOT6_004132"
                     /pseudo
     CDS             2129..2590
                     /locus_tag="ACHOT6_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(2631..3533)
                     /gene="rpnE"
                     /locus_tag="ACHOT6_004133"
     CDS             complement(2631..3533)
                     /gene="rpnE"
                     /locus_tag="ACHOT6_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFP9949258.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3727..4917)
                     /gene="glpC"
                     /locus_tag="ACHOT6_004134"
     CDS             complement(3727..4917)
                     /gene="glpC"
                     /locus_tag="ACHOT6_004134"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFP9949259.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4914..6173)
                     /gene="glpB"
                     /locus_tag="ACHOT6_004135"
     CDS             complement(4914..6173)
                     /gene="glpB"
                     /locus_tag="ACHOT6_004135"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFP9949260.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVGALHAAQQIAQRAGGQQ"
     gene            complement(6163..7791)
                     /gene="glpA"
                     /locus_tag="ACHOT6_004136"
     CDS             complement(6163..7791)
                     /gene="glpA"
                     /locus_tag="ACHOT6_004136"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFP9949261.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
BASE COUNT         1757 a         2050 c         2180 g         1884 t
ORIGIN      
        1 gaaagtgaca gtaatctctt ttgcaatgat aactgcactt attattattt ttgtgtgtat
       61 cggtaaagtg tgtttatttt ttcggcgttt aattatttaa gaagtattta gttacaataa
      121 gtgactagat cccttttttc gctcattgaa tcctctgtgc caggcagtac aatcccgcag
      181 ctttgggaat tttgattaat tgcttgtggg aaaggggata atgaaaaaaa ttgcgctagc
      241 aggtctggcc ggaatgctgt tggtttctgc atcggtcaat gcaatgagca tcagcggcca
      301 ggcgggtaaa gaatacacca atattggtgt cggttttggg actgaatcga cgggcctggc
      361 tttaagcggt aactggacac ataacgacga cgacggtgac gtcgcgggcg tggggctggg
      421 gttgaatctg cctctcgggc cgttaatggc gaccgttggc ggaaaaggcg tttacaccaa
      481 cccgaattac ggcgatgaag gttatgccgc agcggtagga ggtggtttgc agtggaaaat
      541 tggcaacagc ttccgtttgt ttggcgagta ttactactct ccggattcgc tctccagcgg
      601 tattcaaagt tatgaggaag cgaatgctgg cgcgcgttac accattatgc gtccagtcag
      661 tattgaggcg ggttatcgct acctgaatct gtcgggtaaa gacggtaacc gcgacaacgc
      721 cgtggctgac ggcccgtatg ttggggttaa cgccagtttc tgatcccttc cggcgcggca
      781 ttctgtcgcg ccattcagtt tcttactacg ccttatctcc ctttgcgtta tagtgattcg
      841 ataacaaaag caggagataa ccatgttaaa agtggaaatg ctatccaccg gggatgaagt
      901 gttacacggg caaatcgttg acactaacgc tgcctggctg gccgattttt tctttcatca
      961 ggggctgcca ttatctcgcc gcaatacggt gggggataac cttgatgact tagtcaccat
     1021 tcttcgcgaa cgtagtcagc acgccgatgt gctgatcgtt aacggcgggc tgggaccgac
     1081 cagcgatgat ttaagcgcac tcgccgctgc gacagcaaaa ggtgaaggcc tggtgctgca
     1141 tgaagcctgg ctcaaagaga tggaacgcta tttccacgaa cgtggacgag taatggcacc
     1201 gagcaaccgt aaacaagcgg agctgcctgc cagtgctgaa tttatcaata acccggtagg
     1261 caccgcctgt ggttttgccg tgcagcttaa tcgttgcctg atgttcttta ctcccggcgt
     1321 accgtcagaa tttaaggtga tggtcgagca cgaaatcctg ccgcgtctgc gcgagcgttt
     1381 ttctttacca cagccgccgg tttgtctgcg tttaactacc tttggtcgtt cggaaagcga
     1441 tctggcacaa agcctggaca ctctacaact gccgccggga gtaacaatgg gctatcgctc
     1501 ttcaatgccg attatcgaac tgaaactcac cggaccggca agcgagcaac aggcgatgga
     1561 aaaactgtgg ctggacgtta aacgtgttgc cggacagagc gtgattttcg aaggcaccga
     1621 aggactgccc gcgcagatca gtcgcgaatt gcaaaaccgc cagttcagcc tgacgttgag
     1681 cgagcaattc accggtggtt tattggcttt gcaactttct cgcgcaggtg ctccattgct
     1741 ggcgtgtgaa gtggttcctt cacaggaaga aaccctggcg caaactgcgc actggattac
     1801 agaacggcgg gccaaccatt ttgccgggct ggcactggct gtttcgggtt tcgagaacga
     1861 acatctcaac tttgcgctag ccacaccaga cggcactttc gctctgcgtg tgcgtttcag
     1921 cactacgcgc tacagcctgg ctatccgtca ggaagtgtgc gcaatgatgg cactgaatat
     1981 gctgcgccgt tggttaaacg gccaggatat cgccagtgag catggctgga ttgaggttat
     2041 tgagtccatg accttatctg tctgattccg ccactctgtg ccggtttact gcaccggcac
     2101 attccccctc cggcaaaact tgtccaaccc tccctacggt gagcgtggtg ttggggccag
     2161 tgtggcacgg gctgcgcgct ggggacgcat tgagaattac atggcgcaag tcaacgattc
     2221 gctttgtctg ttagtgcagg tggaaagtaa aacggcactg gataacctgg acgagatcct
     2281 cgacgtcgaa gggattgatg gcgtgtttat tggaccagcg gatctttctg cgtcgttggg
     2341 ctacccggat aacgccgggc acccggaagt gcagcgaatt attgaaacca gcattcggcg
     2401 gatccgtgct gcgggtaaag cggctggttt tctggctgtg gctcctgata tggcgcagca
     2461 atgcctggcg tggggagcga actttgtcgc tgttggcgtt gacacgatgc tctacagcga
     2521 tgccctggat caacgactgg cgatgtttaa atcaggcaaa aatgggccac gcataaaagg
     2581 tagttattga tatcaaaggc ccatggggat cggctgtggg cctgtgttaa ttagtggtta
     2641 ttcgctgcca gatcggcttc gcttagctgg gtggccgcga gcacctggtc acgatcaatg
     2701 ccctgttcca gcatgcgcaa agcaatttta atggcttgtt catgcatacc ttcctgccag
     2761 ccaatttgat gcccttcctg ccgtagccgt tcagcaatag tcattaatct ctccttttgt
     2821 agtggtgaac gttcggcaat ctcctcaata aaacgggtga aacgggaggt atcgccgcat
     2881 tgcagcagat aattaaacag tgtttgtagc tggctgtcat tagtgaagcc tctaaccaaa
     2941 agcgtggtga tcctgtcgac catgccgatt aaatcgcggt cgcgaatatg cttttgaatc
     3001 agttccagca gagctatacg ccgatgttgc atgatctcat cgtcaggtac gatggtgata
     3061 tccaccaacg gaaaagcttc ggtgtacaac tgccgggcaa gttgcggatc gtcaaactca
     3121 tccagccagt taagtgagta cgggtagggc gatgtttcgc catgataaaa cagcaatggc
     3181 accaccagcg gaactctgtc atagccttta tccaggtgac gctgcatggc ggcagtggca
     3241 tagcgcatta gccgaaaagc catattcttt tctgcagagc tttgatgttc aatcacgcag
     3301 tagatatagc cgtcaccgtc gctggtttcc acggaccaca aaacatccga gtagtaagcg
     3361 cgtaaacttt tttcaataaa actagtgggt tccaggcgta aggtttgcag gttgcaaagc
     3421 ttgcgcagtg gttctggtaa atgtatttcg agaaaatccc gtgcggtttc gggtgtgaac
     3481 ataaaggttt taaacaccgc atcatgcggg gaggatgttg ttgattcggt catggttgtc
     3541 cgtcacctga tatttatgat gacgcgacat tacgtctatc ccgggccttt gacatctgtc
     3601 ggtttcagcc tttgcgagac attttcagag gaattttcaa agcgttgcaa cgcatacatg
     3661 gtgcgtggaa tcgcgctcgc aaaaatgccc gataagcaat gtgcttaccg ggcatcaaaa
     3721 ggtcggttaa gccagcgcct gggccagtag cgtaatcgga tgttcgcagc gaaggctcgt
     3781 ggacatctca atctgccatt tgcaggtttc gcagtcggtg atgaccagat ccgcgccgct
     3841 ctcttctatc tggcggaaca gtggcgcacc gatggcttgt gaggtggggt agttctcttt
     3901 tttgaaaccg taagtacccg caataccgca gcactgggaa tccagcaccg ttaactcaag
     3961 ccccgggatt ttacgcaaca gctccagggt gtagagcgtc cagcccattt tttccatatg
     4021 gcacggagtg tgataaacca ctttcagcgg cagcggtttc agcggtaacg ttttgccttc
     4081 gtccagcttg cgccacagcc agcgggttgc cagttcgata tgatcgcgca agcctttgtt
     4141 gtcgacattc agcacttccg ggtattcgtc gcgcagggca aatgtacagg ttgaggaggt
     4201 ggcaatcacc ggaatgcctt ttactcccac agcttcgcgg atcgactcta cattcgtaat
     4261 tgcctgtttg cgtgctttat cggtaaagcc gttggcgatt agcggtacgc cgcagcattt
     4321 ttctttgctg agcagttgta caccggtacc cattgcgttg agcactttaa ttaaatcttt
     4381 acctaactgc ggatggttgt agttaacgaa gcagccgtga aagaaagcga cctggtcttt
     4441 atattgtgct tgctgagccg ctatgctgcg ataccagcga cggaacgtgc cgaaggagta
     4501 tttcggtagc gtgcggcgat gatcgatttt taacgccgca tcaagcagct gccgcaccgg
     4561 tttcagcgag gtagcggtgt tgacgattgg tgcgaacggc gtggaaacgc tacccatcag
     4621 gtcggtatga ctcaacacaa aattacgcag cgacgggcgc gtggtgtcat attttgcccg
     4681 cgcgcgctgg ataatatcgc caatcttcac atcggacggg caggcgactt cacaacgttt
     4741 gcagttgatg caatatttca gcgcctcgtc atacagtgcg ccatctttca aacgcagacg
     4801 ctcgccatcc ggcccggctt gttttggccc tggataaccg ggattcaccc ggctcaccgg
     4861 gcaggcggtg gtgcagacgg tgcacttaat gcagttttcg aagctggtgt cgttcattgt
     4921 tggcctcctg cgcgttgggc aatctgttga gcggcatgta aagcaccgac ggcacaaaca
     4981 ccgccgccgc atccctgggc gatgggatca aatccgccca gcaccgaacc gatggcaaac
     5041 aggttctcaa tggtttgccc tgcctgcgac gggcgtagcg tctcatcagt ggttacaccg
     5101 aactgctgcc acggttgcgg cgcaaaaaaa tcgcccttat accattcacc ccgcgtggcg
     5161 gtttgtagca catcaaggcc gagaatcggc tcgcgaatgc cgttacgttc cgctaccagt
     5221 ccgccactaa agaaactgcc gctggcgaga accgcgaaac gtggacgtag cggaatatcg
     5281 gcgtgattgc gggtccagat ttcattcact acgccatttt tacaggtcac ttttttcact
     5341 tcatcgcccg gcatccacac gccaccctgg cgcacaaact ggcgctgtaa ctggttttgc
     5401 agacgaatgc ccagcacgga aggcggcagc gttggcaaaa gcatcagtga acaaggtagt
     5461 ttttcattca accaacgcca cagtttgtca tcggccaggc cgaagcaggc gggcatcagg
     5521 atcatttcgc aggtattggc gacaggaata agcgcatcaa gtaacagcgg ccagttttct
     5581 tcattatcaa ggaaacgggc gatattcacc gcgcgaaatt cggtggcgtt attgcgcagg
     5641 acatccagtt ccggcagctc tatttctgcg gtttcaaccg aaagatccag ttcgcgcaac
     5701 gaagccgccg caagatgcgc ctggaaatcc atcagcccgc ttatccccac tacacatatc
     5761 ttcttcgcgg gtagcggcca gacggggact tctggcgaac ttagccaggt agagcgcaga
     5821 gtgccgagcg gcgtaatccg ctggtgagcc agttctacgc tgccctgcaa ttgcgcaccg
     5881 ctttcagcga ttaatgcctg cgcctggcaa gcgagatcga gcacgcgttg tggcccgaga
     5941 agagagtaag gatgtgcagg tgcctgctga cgcaaagatt ccagtccact gtgaatgtct
     6001 gccacgggtt gaccatctgg cagatggctc agcaaatcca gcgaaccgga tgagaaatgc
     6061 aacgcgcttt gaccacgcgt gacaatggca cagcgcaggc cgtgtttttg cagttgcagg
     6121 ccacagagta atccggcgag gccgccgccc ataatgacag tatcaaagcg catctttctg
     6181 ctccttctcc agaccacaca gtccctgata aacccagcgg gtaaattcgc tttcgcgcag
     6241 tgcgtctccc caggcgatgg gttgcacgcc tttccagcgt tcgttaagga aggtggaaag
     6301 ttgctcgata gattgcgcgg acgtcgtgac gttaaaccgt tgcagcagtc cggcagcgcg
     6361 gcaggcgcag agttcgccct ggcaggtgcc catccccaca cgggtacgac ggcgtaaatc
     6421 cagcaggcta ttaacgttta aattttctac cgcgtactgc acttcacccg cagttaccgc
     6481 ttcgcactca cataccaggc tacggtgcag acggccttcg ctcagccagg caggcgtgcg
     6541 atcgccatga cgataaaccg cagaaccgcg cagcggggca ggcagggaga tgactttacg
     6601 caaggtaact tcagcgggtt cttgtgaacc aggcagtgcc agatcggcag tcgtacaggg
     6661 gcgcgtgttg cccagtttgc ggcataccgc gtcggtagcc cattcagcca tcagccgata
     6721 ggtcatcagt ttgccaccgg tgatggtgat aaatccgtcc agaccatcgc gttcagcatg
     6781 gtcgagcagc acgatgccac ggctgacgtt acgtccgctc gggtcgtcat cgctggcaac
     6841 cagcgggcgc acgccagaat aggcccgcaa aatgcgcgtt ttcgccatca cgggggccag
     6901 tttttcccct tcacgcagca gaatatcaac ctcttctgcc gtcactcgat tatcgtcaat
     6961 ctcgttgtaa tcaatacgta aagaggtggt gccaatcagc gaaatggtat cgccaggcac
     7021 cagaatatcg gcgtcggaag gtttacggca gcggttgatc acatgctggt taatgcggtg
     7081 atccatgatc agcagcgatc ctttcgccgg gaacatgcga atgcgcagat cggcatattc
     7141 ggcaatgtgt tgcccccaga tcccagcggc attaaccacg acaggtgcat gaagggcctg
     7201 agtttcgccg gtgagatggt tacgtacacg aacaccgcac accgtcgcgc cttcacgaat
     7261 cagccccgtg acttcatgag cggtaaggat aacggcaccg tgttctttgg catccagcat
     7321 gtttgctgcg gtcagacgaa atggatcaac ggtgccatcc ggaactttca ccgcgccaat
     7381 cagtgccggg ttaacggcag gttcgataat gcgcgcttgc tgcgggtcta tagcttctgc
     7441 gctgatccct gcttcttcgc aggcgcgaat aaaagtggcc tggaaggaga ggtcatcttc
     7501 cggcagggtg ataaacaggc cgttggttgg ttcaacgcag tgacgtgcaa tgcgtttcag
     7561 gatctggttt tcactaatgc attcgcgggc cgattccgca tcggttaccg catagcgcgc
     7621 accgctgtgc agcaggccgt ggttacgccc ggtggcaccg gttgcgatgt cgtggcgctc
     7681 aaccaaaatc acgcgcagcc cgcgcagggc acagtcgcgg gcaatcccgg ctcccgttgc
     7741 gccgccgcca atgataatca cgtcacttga ttgcgagtcg cgagttttca ttgttatccc
     7801 tctgaagttc gttttttacc atttagccat agtaaaaaca tgaattgttt gatttcgcgc
     7861 atattcgctc a
//
LOCUS       DAZKIV010000178         7860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZKIV010000178 DAZKIV010000000
VERSION     DAZKIV010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.178"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..564
                     /gene="lpfA"
                     /locus_tag="ACHOT6_004137"
     CDS             40..564
                     /gene="lpfA"
                     /locus_tag="ACHOT6_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial major subunit LpfA"
                     /protein_id="HFP9949262.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAPGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     ISVSTEPGAATGVGIGIYDNPNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTI
                     GYGNAEVDFNLTYN"
     gene            648..1340
                     /gene="lpfB"
                     /locus_tag="ACHOT6_004138"
     CDS             648..1340
                     /gene="lpfB"
                     /locus_tag="ACHOT6_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFP9949263.1"
                     /translation="MKRISLVVLAFLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNN
                     QIVVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNANEVKYSIINDF
                     GVAGEMLTKPIN"
     gene            1364..3895
                     /gene="lpfC"
                     /locus_tag="ACHOT6_004139"
     CDS             1364..3895
                     /gene="lpfC"
                     /locus_tag="ACHOT6_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFP9949264.1"
                     /translation="MHWMHLPLYHSRAHFSFSLLALTIASALPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKTSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     LITARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3906..4961
                     /gene="lpfD"
                     /locus_tag="ACHOT6_004140"
     CDS             3906..4961
                     /gene="lpfD"
                     /locus_tag="ACHOT6_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFP9949265.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDVLTKIYVYTQLDIPVPFTDRSNVTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4967..5491
                     /gene="lpfE"
                     /locus_tag="ACHOT6_004141"
     CDS             4967..5491
                     /gene="lpfE"
                     /locus_tag="ACHOT6_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFP9949266.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5744..7435
                     /gene="eptB"
                     /locus_tag="ACHOT6_004142"
     CDS             5744..7435
                     /gene="eptB"
                     /locus_tag="ACHOT6_004142"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFP9949267.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            7527..7603
                     /locus_tag="ACHOT6_004143"
     tRNA            7527..7603
                     /locus_tag="ACHOT6_004143"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7561..7563,aa:Pro,seq:cgg)
     gene            7638..7771
                     /locus_tag="ACHOT6_004144"
     ncRNA           7638..7771
                     /ncRNA_class="other"
                     /locus_tag="ACHOT6_004144"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
BASE COUNT         2052 a         1804 c         1987 g         2017 t
ORIGIN      
        1 caacatcaag catatttatc tatataaatg gagttataaa tgaaaaaggt tctgtttgcc
       61 ttatctgcgc ttgcgctgac ctcaacttcc gtattcgcag ctgatgcggg cgacggtact
      121 gttaaattca ctggtgagat tgttgacgcc gcttgtgttg tgtctccaga cactcaaaaa
      181 caagaagttg ttctgggaca ggtgaacaag tctgtgttta ctgctcctgg tgataaatcc
      241 acagcaactc cgttcaaaat caaactggaa aactgcgata tctccacttt caaaaatgtg
      301 gaaatcagct ttaacggtgt tggcgatgca gataacagca aactgatttc tgtaagcact
      361 gaaccaggtg ctgcaactgg cgtaggtatc ggtatttatg ataaccccaa taccttggtt
      421 gacctgaata ccggtaaatc tgctaccgtc ctgaaagaag gccagaccgt tctgtatttc
      481 accgctaact atgtctctac taaagatgct gtaaccatcg gttacggtaa cgccgaagtc
      541 gacttcaacc tgacttacaa ctaatcgatc ctgacggaac gatcatctct caatggcaac
      601 agagactctg ttgccattat tcacaccaat gactcaggga ccgaatcatg aaacgtatta
      661 gccttgtcgt gctggcattt ttaatgaccg cacaacagtg ttttgctggt ggcgtagcct
      721 taagtacgac gcgtgttatt tatgatggcg gcaagaaaga agcgtcgctg accgttgaaa
      781 accataataa aaatgaagag tttttgattc agtcatggat tgatgacgtg aatggcaata
      841 aaaaaacacc atttattatt acaccgccgt tattcaaact tgatcctgat aaaaataacg
      901 tcttacgtat cgtcaatatt gaagatactt tacctaaaga tcgtgaatcc gtttactgga
      961 ttaatgtaaa agccattcct gcgaaaagcg ctgaaaatga aaatgaaaat aaaaacgtct
     1021 tacagatagc cgtgcgcacc cgtctgaagt tgttctaccg tccaaaaggt ttgccgggaa
     1081 gcaacctcga ggcaatgaaa cagttacgtt tccaacgtca aaataaccaa attgtggtta
     1141 ataacgactc cgcttataac ctgactttta atcagttttc catcaatgga caaaagattg
     1201 aacgggcggg aatggttctc gcgaaaggaa agttaaacat taaccttcct gccggtacag
     1261 gcaacgcgaa cgaagtgaaa tacagcatta ttaatgattt cggtgttgct ggcgaaatgc
     1321 tgacgaagcc gattaactga tccttttaca aggcgctttt actatgcatt ggatgcattt
     1381 acctctctat cactccagag cgcatttttc cttctcactg ctggcattaa cgattgccag
     1441 cgcgttacct gcgtatggcg gaaaatttaa ccctaaattt ctcgaaaacg ttcagggtat
     1501 cgatcaacac atcgacttgt cggtatacga ttcacctgtc ggtcagcaaa tccccggtaa
     1561 ataccgtgtt tcggtgtttg taaacgaaga aaaaatggct tcccggacgc tggattttag
     1621 taccgccagc gaagcgaaac gcaaagccag cggtgagtcg cttatgcctt gccttagccg
     1681 ggtgcaacta gaagaaatgg gcgtgcgcgt tgacagcttt ccggcgctga agacgtcacc
     1741 accagaagcc tgcgtagcgt ttgatgaaat cattcctcaa gccaccagcc gctttgattt
     1801 taatactcag acgctgcatc tttcctttcc acaggcggca atgttgatta ccgctcgcgg
     1861 cacggttgat ccttctcgct gggacgaagg aatacctgcg ctgttgctgg attacagctt
     1921 tagtggcagc aatggacgca atgaaggtac aggttcatct tcagattcga ccagtgacag
     1981 ttattacctg aacctgcgca gcggtttgaa cgttggtccg tggcgtttac gtaataacag
     2041 tatctggaac cgtaccgatg gcaaaaacca atgggataac gttggtacat cgttaaaccg
     2101 ggcgattatt ccactcaagt cacagataac attgggtgat accgctacgc ctggagagat
     2161 ttttgacagt gtacaaatgc gcggggcgtt actggcatcc gacgatgaaa tgttgccgga
     2221 tagccagcgt ggctttgctc cggtggtgcg cggcatcgcc aaaagcaacg ctgaagtgag
     2281 tatcgaacag aacggatatg ttatttatcg tacctttgtt cagccgggcg catttgagat
     2341 taacgatctc tatgcaacct ccggcagcgg cgatctgacg gtgatcatta aagaagccga
     2401 cggaagtgaa caacgtttta tccagccgtt ttcggcagtg gcgattttcc agcgtgaagg
     2461 ttatctgaaa tacagcctgg ctgccggtga gtatcgtgct ggtaattacg acagcgataa
     2521 accacatttc gggcagttta ccgccatgta cggcttgccg tggggtatga ccgcctacgg
     2581 tggtgcattg ttgtcggcag attacaacgc tttagctctg ggcctgggga aaaacttcgg
     2641 taccattggc gcagtatcgg tggatgtcac tcaggcgaaa agtcagttac gcaacaatga
     2701 aaaagaggaa ggccagtctt atcgcttcct ctattcgaaa agttttgagg gtggcactga
     2761 cctgcgtctg ttgggctaca aatactcaac cagcggctac tacaccttcc aggaagcaac
     2821 tgatgttcgc agtgacgccg acagtgatta ccgccgttat cacaagcgca gccagataca
     2881 gggcaatatt acccagcaac tgggagacta cggctcggtt tatttcaata tgacgcagca
     2941 ggattactgg aacgttgacg gtaaggaaaa ctccttaagc gccggttatc acggtcacat
     3001 tggtcgggtg aattacagcg tggcctatac ttggacgaga agtccggaat gggaagaaga
     3061 cgatcgcttg tggtcattca gtgtttccat ccctctgggg ggcgcgtgga gcagttatcg
     3121 catgactacc gaccagaatg gcaagacgtc gcaacaggcc tctgttagcg gcacgttact
     3181 ggaagatcgt aacctgagct ataacgttca gcagggttac accagtaatg gcgtaggcta
     3241 cagtggcagc gtcaacatgg ggtatatggg tggctctggt aacattgacg taggctataa
     3301 ctattctaaa gataaccaac aggttaacta tggcgtgcgc ggcggtgtca tcgttcacag
     3361 tgaaggtata acgctttcgc agcctttagg cgaaagtctg gcgattgttt ctgcaccggg
     3421 cgcgcgtggt ggacatgtgg ttaacagcag cggcgtagaa gttgactgga tgggcaacgc
     3481 cgtggtgcct tatctcacac catatcgtga aaccattgtg gaattgcgta gcgacacgtt
     3541 ggggcaaaac gttgaactac aggaagcgtt ccagaaagtc gtgcctactc gtggtgcagt
     3601 tgtgcgttcc cgctttgata cccgcgtcgg ttaccgtgtg ctgatgagct taaaacaggc
     3661 gaatggcaac gcagtaccgt ttggtgcaac cgcagcgtta attgatgaga gcaaacctgc
     3721 cagcagcatt gtgggtgaag aaggacagct ttatatcagc gggatgccag aagagggcga
     3781 attgcaggtg agctggggga acgagcaggc gcaacgttgc cgtgtgccat tccgcttgcc
     3841 agagaacaag gacaatgcgg ctatcgtgat ggttaatgcg gtatgcgaga aataaggata
     3901 tttagatgaa aattaaaaat ttgttggcag gtatggtgct gttagggacg agcacttttg
     3961 ctggaaatat atgggctgcg gattggggac catgtcagac atcaaatggg gtggcccatg
     4021 agtatagttt tgatttcgta cagactatcc aggtaccttc agaaaataag gctgggaaaa
     4081 tattaactca gccctttgct ttaggtacaa aatattccgc ttattgtgaa tgcccggatc
     4141 ccattccaga taatggagtt gtgacttatt ttaaaggtgt aacgcttctg cctgagcctg
     4201 gaactgtaga tgggtactat aaattcaata attacattga tgttctcacc aaaatatatg
     4261 tctacacaca attagatatc ccggtgcctt ttaccgatcg aagcaatgtc acggcacaga
     4321 aagaatgtac gccctatact gctaacaatt gggggacagg tggtaaagga agtatcagta
     4381 tttatatttc acatcctttt gttgggcaaa tgattattcc taaaacacga gttgcgtccc
     4441 tctttgggac aaaaaagaaa ggtgtttaca atgatagtca gcccatggcc aatgtcagta
     4501 tttccgggag tattacagtt actcaaggct gtgaacttgc cgctggcacc gtactggata
     4561 ttccattcgg agaatatcag gcccatgatt ttaaagggcg tgccggacaa ccaccacaaa
     4621 atgttcagaa ggtacaaaaa gaattaagct ttgactgcaa taatatttca gatggcgtca
     4681 aaatttatct cagtattgat gcaacaccaa atactgccta tcccagcgca attgatttag
     4741 gcaacgccga tgttggtgca gtcattgagg acggtaaagg taatattctt aagccgaatg
     4801 acagtaatag cttattagaa atgaacccag gctcgctgta tgaagatgtc aaacgcaaag
     4861 cgacaaccac aattaccgct tatcccgtta gcactaccgg aaaattaccc gctgccgggg
     4921 attatagcgg tattgctacc atacatgtcg aactggagta acagtcatga agcgaacaat
     4981 acttgcgtta tgcctgctgc ctctttcctt ttcgtcattc gcggcaacca cggatttagg
     5041 gccgaaagga acattgaaat tcacccttaa aatcagccag ggtgcttgcg aactggtgaa
     5101 agacagcgtt gaggttgaca tgggcaccgc ggttctcaaa aagccggtaa gaatcggaac
     5161 ggaaattaat cccactcctt tttccatcgg cctgaaaaac tgtagcgaag tggtcagagc
     5221 ctacgtgact atggacggta cacccaatgc agacgactca aatctttttg cgctggatgc
     5281 gggtggcgct acaggcgttg ggctgaaaat caaaaccgct gctggagtac aacagttccc
     5341 ggaaaataca acgccgacac cggttgagtt tgcaattaac ggtgatggcg cacatcaact
     5401 gaactatatc gccagctacg tgcctattcg cgccgatgca actattggtc gcgcagatgc
     5461 aacggtcgac tttactgtac agtacgaata atttcacatc ttaagggcca gttaactggc
     5521 cctttttact gctttttgta acgctaaagt ctcttttcaa acttgcattt ttgtaaattt
     5581 gtgcttcatg cacactcttt ccccacactt tttcgctttg ctgtggtcta cttattcgcg
     5641 cgtgtagatt ttacttatct gactacctcc gcacttttcc cctgccgggc ctgaaaagcc
     5701 gctaagcagg gtgttatcac ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc
     5761 gattacacag cagaagctga gctttttgct tgcaatctat attggccttt ttatgaatgg
     5821 cgcggttttt tatcgccgct tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt
     5881 ttctgctgtt gttgaactgg ccgccaccgt actggtgacc ttctttttac tacgtcttct
     5941 atcgctgttt ggccgccgca gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc
     6001 aggtgccagc tattacatga ccttccttaa tgtggtcatt ggttatggca tcatcgcttc
     6061 cgtcatgacc accgatatcg acctgtcaaa agaagttgtt ggtctgaact ttattctctg
     6121 gttaatcgcc gttagtgcat tgcctcttat ccttatctgg aataaccgct gtcgctacac
     6181 cttgctccga caactgcgaa ccccagggca gcgtattcgc agcctggcgg tcgtcgtact
     6241 ggcgggtatt atggtttggg caccgattcg tttgctggat atccagcaga agaaagtgga
     6301 gagggcgacc ggcgttgatt tgccgagtta tggcggtgtc gtagcgaact cttatctgcc
     6361 atcaaactgg ctttctgcgt tggggctgta tgcctgggcg cgggtcgatg aatcttccga
     6421 taataattca ttgcttaatc cggcgaagaa attcacttat caggcaccgc aaaacgttga
     6481 tgacacttat gtcgtgttta tcatcggtga aaccacgcgt tgggaccata tgggtatttt
     6541 cggctatgag cgtaatacca cgccgaaact ggcccaagag aaaaatctgg cggcgttccg
     6601 tggttactcc tgtgataccg caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg
     6661 gggcgcggaa gataatccgc agcgcacatt aaaagaacag aacattttcg cggtgctgaa
     6721 gcagttagga ttcagttctg acctctacgc tatgcagagc gaaatgtggt tctacagcaa
     6781 cacgatggcg gacaacattg cttatcgtga gcagattggt gcggagccac gtaatcgtgg
     6841 caagccggta gatgatatgt tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc
     6901 ggatggtaag catctgatca ttctgcatac caaaggttcg cattttaact acacccagcg
     6961 ttatccgcgt agcttcgcgc agtggaagcc ggaatgtatt ggtgttgata gcggctgtac
     7021 caaagcgcag atgatcaact cctatgacaa ctcggtgacc tatgtggatc actttatctc
     7081 cagcgtgatt gatcaggttc gcgataagaa agcgattgtg ttctacgcag ctgaccacgg
     7141 tgagtcaatt aatgaacgcg agcacctgca cggcacgccg cgtgaactgg caccgccgga
     7201 gcagttccgc gtaccgatga tggtctggat gtcagataaa tatctggaaa atccggccaa
     7261 tgcgcaggca tttgcgcagc tgaaaaaaga agccgacatg aaagtgccac gccgtcacgt
     7321 agagctgtac gacaccatca tgggttgtct tggctatact tcaccggatg gtggaattaa
     7381 cgaaaacaac aactggtgtc acatcccgca ggcaaaagag gcagcggcta actaagcggc
     7441 ctgctgactt tctcgccgat caaaaggcat tttgctatta agggattgac gagggcgtat
     7501 ctgcgcagta agatgcgccc cgcattcggt gattggcgca gcctggtagc gcacttcgtt
     7561 cgggacgaag gggtcggagg ttcgaatcct ctatcaccga ccaaattcga aaagcctgct
     7621 cagcgagcag gcttttttgc atttgaagcc catgaggatg agaacctccg ggggcgggga
     7681 ggttcgactc gagcgaagcg agagaacgtt gcgccagcaa cggcccgcag ggcgagccac
     7741 gaagtggcga gtaatcctct atcaccgacc aaattcgaaa agcctgctca gcgagcaggc
     7801 tttttttgca tttgtagccc atgaggatga gaacctccgg gggcagggag gttcgactcg
//
LOCUS       DAZKIV010000179         7748 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZKIV010000179 DAZKIV010000000
VERSION     DAZKIV010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.179"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..347
                     /locus_tag="ACHOT6_004145"
     CDS             135..347
                     /locus_tag="ACHOT6_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9949268.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEPVNDIPAHCDKS"
     gene            304..1296
                     /locus_tag="ACHOT6_004146"
     CDS             304..1296
                     /locus_tag="ACHOT6_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949269.1"
                     /translation="MSLLMTSQPIVINRDLACRIGLNEAIVLQQLHYWLNETNSGTEH
                     GGIRWVYNTTEQWLEQFPFWSESTLKRTFASLKSLGVLRREQLNKSKRDMTNFYTINY
                     ESELLEEVKVNESIRSKCTSPSGQSDLMDGRKMTRSIGSKRHAVIGSKWPNDLTENTT
                     EITTENKTSSRPDASQPDTQTAEQEFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKK
                     IIALYEQAAECDGEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWC
                     RNVLSPSKLREKWDELSLRLSPSVSTHTEKREDPYFKASYDNVDYSQIPAGFRG"
     gene            1293..1376
                     /locus_tag="ACHOT6_004147"
                     /pseudo
     CDS             1293..1376
                     /locus_tag="ACHOT6_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1391..2044
                     /locus_tag="ACHOT6_004148"
     CDS             1391..2044
                     /locus_tag="ACHOT6_004148"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9949270.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            2041..2367
                     /locus_tag="ACHOT6_004149"
     CDS             2041..2367
                     /locus_tag="ACHOT6_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP9949271.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIHSLLAGEENARLRATHWLH
                     ERGLKV"
     gene            2364..2759
                     /locus_tag="ACHOT6_004150"
     CDS             2364..2759
                     /locus_tag="ACHOT6_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9949272.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKASCKLATR"
     gene            2911..3726
                     /locus_tag="ACHOT6_004151"
     CDS             2911..3726
                     /locus_tag="ACHOT6_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9949273.1"
                     /translation="MNQLLVIDGVSVRQYFESNYCLNDLQKAALLAAGENRSSRSLEV
                     HEFMRRPETKALVELLEEETTGDSRSIPVITIQGRNGGTYVCKELVYAYAMWISPAFS
                     LKVIRTFDALHNSSPEETISDKIKSGVILLESAAKTLNLSNSSKLGAYQKLSKVAGLP
                     ELMPIYAIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYS
                     RTAINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3742..4257
                     /locus_tag="ACHOT6_004152"
     CDS             3742..4257
                     /locus_tag="ACHOT6_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949274.1"
                     /translation="MRITPPHLQPVLSRVKRFVERMPEGATLTQISQKVQAYSLLNKR
                     DKEILIGIIRDSGLLVVANDGRTTTLHHPKFGHQAVNSEIPVKTEEPVVIKKTVTPDE
                     LRRHAEELIRAAEEAEKKFNDRAEIKKQLDPLKLEILQAYGMASRKFDEFVDAMADMG
                     KAVQKLKQIVL"
     gene            4267..5256
                     /locus_tag="ACHOT6_004153"
     CDS             4267..5256
                     /locus_tag="ACHOT6_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9949275.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRHEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLEAMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLAYALPESAARKALRLPKPVVPSVTRESDLVPSVTATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLS"
     gene            5274..5654
                     /locus_tag="ACHOT6_004154"
     CDS             5274..5654
                     /locus_tag="ACHOT6_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9949276.1"
                     /translation="MRDIQMVLERWGAWAASGNTGVDYSPIAAGFKGLLPSATKPRPA
                     CCDDDGLIIENCLARLKQKKPEEYSLLIAHYLLRISKRQIARTRKKSEKAIRIEMQIA
                     EGFIDGCLSVLGVRLEMDDWLLKK"
     gene            5870..6022
                     /locus_tag="ACHOT6_004155"
     CDS             5870..6022
                     /locus_tag="ACHOT6_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9949277.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLSAG"
     gene            6009..6404
                     /locus_tag="ACHOT6_004156"
                     /pseudo
     CDS             6009..6404
                     /locus_tag="ACHOT6_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            6445..6520
                     /locus_tag="ACHOT6_004157"
     tRNA            6445..6520
                     /locus_tag="ACHOT6_004157"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6478..6480,aa:Ile,seq:cat)
     gene            6601..6677
                     /locus_tag="ACHOT6_004158"
     tRNA            6601..6677
                     /locus_tag="ACHOT6_004158"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6635..6637,aa:Arg,seq:tct)
     gene            7201..>7748
                     /locus_tag="ACHOT6_004159"
     CDS             7201..>7748
                     /locus_tag="ACHOT6_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9949278.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPDWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGA"
BASE COUNT         1996 a         1782 c         2160 g         1810 t
ORIGIN      
        1 cttcctgtac cgggtggaaa agcgaaggtg gagtattgct atgcagtgaa tgtaccaggt
       61 gacagggaaa ttgtaaccca cagctttgca gagtgggctg tgggtgattt caaccggcag
      121 aaggagacag tcctttgcga caagttaacc gctggttcaa agatcactac ggagtgcccg
      181 tcagagtcat tcgttgggag ccggaaacac aacgggttat ctacctccgt gaaggctatg
      241 aacatgaatg cttcagtccg ctcgaacagt ttcgtcgtaa attcagggaa atagaggtcg
      301 gtcatgagcc tgttaatgac atcccagccc attgtgataa atcgtgatct tgcatgccgt
      361 attggtctga atgaggcaat tgtgttgcag cagcttcatt actggctgaa tgaaacgaat
      421 tcaggcactg agcatggcgg aattcgctgg gtttataaca cgacagaaca gtggctggag
      481 cagtttccgt tctggtcaga gtccactctg aaacgcacat ttgcaagcct gaaatcactt
      541 ggggttttgc gtcgcgagca actcaataaa tcgaagcgtg acatgaccaa cttctacacg
      601 atcaactatg aaagtgagct tttagaagag gtcaaagtga acgaatccat caggtcaaaa
      661 tgcacttctc catcgggtca aagtgacctg atggatgggc gcaaaatgac acgatccatt
      721 ggttcaaaac gacacgctgt catcgggtca aaatggccca atgatcttac agagaataca
      781 acagagatta ctacagagaa taaaacctct tctcgtccgg acgcttcgca accggacacg
      841 caaacggctg aacaggagtt tttaactcgc catcctgatg cggttgtatt cagccctaaa
      901 aagcgccagt ggggaacgca ggatgatttg acctgcgcac agtggctctg gaaaaaaatc
      961 atcgccctgt acgagcaggc cgccgaatgt gacggcgagg tggttcgtcc caaagaaccg
     1021 aactggacag cctgggcaaa cgaaattcgc ctgatgtgtg tgcaggatgg tcgtactcac
     1081 aaacaaatct gcgagatgta cagccgcgtc agccgcgatc cgttctggtg ccgtaacgtg
     1141 ctcagcccgt cgaagttgcg ggaaaaatgg gatgagcttt ccctgcgctt atcgccgtcc
     1201 gtcagcacgc acacagaaaa acgtgaagac ccgtacttca aagccagtta cgacaacgtg
     1261 gactacagcc agatcccggc aggattcagg gggtgatcat gagtctgtta aatgacgttc
     1321 agaaattcat tgaagcccat ccgggctgta cttccggaga cattgcggat gctttttacg
     1381 tgggggctta atgagtaata aatattgcca ggcgctggtg gaactgcgga ataaaccagc
     1441 ccatgaactg aaggaagtgg gagatcagtg gcgcacgccg gataacattt tctggggaat
     1501 taacaccctg tttggtccgt ttgttctgga tctgttcact gacggtgata acgccaaatg
     1561 tgccgcgtat tacacggcgg aagacaacgc gctggcgcat gactggtcag aacgccttgc
     1621 ggagcttaaa ggtgctgcct ttggtaatcc tccatacagc cgcgccagtc agcatgaggg
     1681 gcaatacatc accggcatgc gttacatcat gaaacatgcc agtgccatgc gtgataaggg
     1741 cgggcgctat gttttcctga tcaaagctgc caccagcgaa gtgtggtggc cggaagatgc
     1801 agatcatatt gcttttattc gcgggcgtat tggttttgaa ctgcctgtct ggtttatccc
     1861 gaaagacgag aagcaggtac cgacaggagc ttttttcgct ggtgctattg ctgtttttga
     1921 caagacctgg aagggaccgg caatcagcta cattgggcgc gatgaacttg aggcatgtgg
     1981 tgaggcgttt ctggcgcagg ttcgccagca ggcggaaaaa ctggtcaggg agatggcggc
     2041 atgacgacat taactcaatg ccagcagcag gtgctggata tgctgatttc ttatcagaaa
     2101 gagcgtggct ttccgccaac caatcaggag gtggcaacca tgctgggata ccgttcagtg
     2161 aatgcagcgg tagagcatct tcgcgcactg gagaaaaaag gcgtcatcac gataaagcgt
     2221 ggcgtggccc gggggataac gcttcatacc gcggtgaagg acgacgacag cgaggcggtc
     2281 gggattatcc actcactgct tgccggtgag gaaaacgcaa ggctgcgtgc aacccactgg
     2341 ttacatgaga ggggcctgaa agtatgaagc tgatcctgcc ttttccgccc agcgtgaaca
     2401 cgtactggcg acaccccaac aaaggggcgt ttgctggtaa gagcctgata agcgcggcgg
     2461 ggcgcaaatt ccagagcgcg gcgtgtgcag caatagttga gcagttacgt cgtctgccaa
     2521 aaccaacgtc ggcacctgca tcagtggaga tcgtgttgtt tcctccggat aaccggatcc
     2581 gcgatctgga caactataac aaggcgctgt ttgacgccct gacccacgcg ggtgtgtggg
     2641 aagacgacag tcaggtgaaa agaatgctgg tggagtgggg accggttatc ccggaaggga
     2701 aggtcgagat cactatcagt aagtacgaaa aagcgagttg caaattagca actcggtaac
     2761 ggaattgagc aacaccctaa atttgggtat tacctcgtta aagatactgt atttatgaac
     2821 agtgtatcct tgataactat taaaaatcgc agtaagttca tcttgcatca acgaaaaggg
     2881 agtgcagtcc cgaaatttgt ggagaaacca atgaatcagt tgcttgtaat tgatggcgtt
     2941 tctgtgcgcc agtactttga atctaactac tgtcttaacg accttcagaa agctgctctt
     3001 cttgccgctg gtgagaatcg ctcctcccgt tcgctggaag ttcacgagtt tatgcgtcgt
     3061 cctgaaacga aggctcttgt ggaattactg gaagaagaaa ctacgggaga ttcccgtagt
     3121 attcctgtca tcaccattca ggggcgcaat ggtgggacgt atgtctgtaa agagctggtc
     3181 tatgcatatg caatgtggat cagcccggca ttcagcttaa aagtgatacg tacttttgat
     3241 gcgcttcata attcatcacc agaagaaacc atatccgaca aaattaaatc cggggtcatt
     3301 ctgcttgaat cagcagcaaa gactctaaat ctgtcaaact cctcgaaact tggtgcatac
     3361 cagaaattat caaaggtagc aggtcttcct gaacttatgc cgatctatgc cattgatgca
     3421 cctgctgatg cgccagatgg ttcaagccgc ccgacactgt cactgagtgc actgctgaaa
     3481 cagtatggta tacgcctgac ggctaatcag gcatatcacc agatggtgaa actggggatc
     3541 gtcgagcagc gcgaacgata cagccgtacc gcgattaaca acatcaaaaa attctggtcg
     3601 ctgacagcga aaggttgcat gttcggcaag aacatcacca gtcctgcaaa tccgcgcgag
     3661 acgcagccgc atttcttcga atcccgattc cctgagctgt taaagctgct cgataccgtt
     3721 cactgatggg aggcgtggag catgagaatt acaccacccc atctgcagcc agttttatcc
     3781 agggttaaac gttttgttga acgaatgccg gaaggcgcaa cactgaccca gatatcacag
     3841 aaagtgcagg cgtacagtct actgaataaa agggataagg agatactcat tggcattatc
     3901 cgcgacagtg gacttctggt cgttgcgaat gatggaagaa ctacaacgtt acatcatcct
     3961 aaatttggac atcaggcagt aaatagtgaa ataccagtaa aaacagagga acccgttgtg
     4021 attaaaaaga ccgttactcc ggatgaatta cgcaggcatg ctgaggagct gatcagggct
     4081 gcggaagaag cagagaagaa atttaatgat cgtgcggaaa ttaaaaagca actggatcct
     4141 ctgaaactgg aaatcctcca ggcgtatgga atggcaagtc gtaaatttga tgagtttgtt
     4201 gatgctatgg cggatatggg gaaagcggta cagaaactga aacagattgt gctgtgaggt
     4261 tctacgttga gagcactgct gacccctgaa attgccccgc gtatggggat cgtcttgttc
     4321 agaccaggtt cagagctgat gcccctgttt atgcaggggc gtgtcctgct ggagcctgag
     4381 ccggaacgtt attcatcttt tgccagtggt gccgttccgg cagcatcaca accgctggcg
     4441 gatgatcctg ccgttcgggc cgtgttccgc catgaggcgg tgatccgtcg tgctggtggc
     4501 gtggaatgtc ttgaaagctg gttacttcgt gaaaaaggct gccagtggcc tcattccgac
     4561 tggcacagcg agaacatgac cacaatgcga cacgctccgg gcgcaatccg tctgtgctgg
     4621 cactgcgata accagctgcg cgatcagttc acggaacggc tggaagcaat ggcaacggat
     4681 aactgtgccc gctgggtgtt gtctgttgtg cgtcgggatc tcggttttga tgatagtcac
     4741 gttgtgacaa tgccggaact gtgctggtgg ctgattcgta atgacctggc gtatgcctta
     4801 ccggaaagtg cagcccgtaa ggcactgaga ttaccgaagc ctgttgtgcc gtctgtcacc
     4861 cgggaaagtg accttgtgcc ttcggttact gccaccagca tcatccagga taaggcgaaa
     4921 aaggtgctgg cgctgaaagt ggatccggag tcgccggagt cttttatgtt acgccccaaa
     4981 cgtcgtcgct gggttaatga aaagtacacg cgctgggtta agacacagcc ttgcgcgtgt
     5041 tgtggtaagc cagccgacga tccacatcac ctgattggcc atggtcaggg tggaatgggt
     5101 acaaaagcgc atgacctttt tgtgttgcct ttgtgcagaa aacaccatga cgaactgcat
     5161 gcggataccg tggcatttga agagaagtat ggttcccaac tggagctgat atttcgtttt
     5221 atcgatcgcg cgctggcgat tggtgtgctg tcctgatttt gtggagaaag ttgatgcgtg
     5281 atattcagat ggttcttgaa cgctgggggg catgggcggc aagtggtaac accggggtgg
     5341 actattctcc gatcgctgcc ggattcaaag gacttttacc atctgccact aaaccacgtc
     5401 cggcctgctg cgatgatgac ggacttatca ttgaaaactg tcttgctcgt ctgaagcaga
     5461 aaaaacctga ggagtattcg cttctcattg ctcattattt gttgcgaata tcaaaaagac
     5521 agatagccag gacgagaaag aaaagcgaaa aagcaatacg aattgagatg cagatagccg
     5581 aagggtttat tgacggatgt ttgtctgtgc tgggggtaag actggagatg gacgactggc
     5641 tgctaaaaaa gtaaaaaatg attagtgcgg tccgcaaaaa gtatgtcagt atgttaagag
     5701 tggttacttc gccacacagc ttaaacccgc cgcgagcggg tttttttatg gctgaaatcg
     5761 gtccagtaca gtaaacgtgc tggtggcggt gaatacctgt ctttcagctt gccggctttt
     5821 tcgacaagag ttattggtgt gtcacgttaa ccggaaaagg gaaaaagaca tgctgaaaca
     5881 gcaggatatg acagaaaccg ccagagtggt gtttaatgaa ttaagcgtca ccgaaccggc
     5941 gacagtcggg gagattgcgc agaatactta cctttcacgc gaacgctgtc agttaatact
     6001 gacccaacta tcagcggggt gaactggaaa agccacatca ccaactggtc agcacataca
     6061 gtgagctgaa ccggcagtat acggaactgc tgagtgaata taaaaatttg cggcggtatt
     6121 tcggtgtgac ggtgcaggtg ccgtacaccg atgtgtggac gtataaaccg gtgcagtact
     6181 atccagggaa acatccgtgc gaaaaaccgg cagaaatgtt gcagcagata atcagcgcaa
     6241 gcagtcgtcc gggagacctg gttgcagatt tcttcatggg gtcggggtcg acagtgaaag
     6301 cagcgatggc gctgggacgt cgtgcaactg gcgttgaact ggagactgaa cgttttgagc
     6361 agacggtgcg ggaagtacag gatttaatca ttcgtaacgg atgagattgc ggagttaatc
     6421 atgcgtcgtt attattcagc aatcggccct ttagctcagt ggtgagagcg agcgactcat
     6481 aatcgccagg tcgctggttc aaatccagca agggccacca accgccacta gctcatcagg
     6541 aaagaacgtc accctgtgcg agattcggag tccccggtgg cggtccatta tcggtattct
     6601 gcgttgttag ctcagccgga cagagcaatt gccttctaag caatcggtca ctggttcgaa
     6661 tccagtacaa cgcaccacac cacacttatc tgccctgact ctcttttgcg ggctttttat
     6721 tacaggaaag acaccggaca gtgaaatgtt aaatgcctca caattcaggc agttgactgt
     6781 tgcctgacat gctgagcgtt tgttaaaaaa atcctgcatg atgaatcccc ctgagcggcg
     6841 gggcataatg acagatgttt ggttgcgtat tgtataggca agttgcggat tctgtctggt
     6901 cattgcagaa ttcaccggga ggcacccggc atcatgctgt atacagagat taggcatata
     6961 tccaggcttc tcatcgcagg agccttttta catgcaaaaa aaagcccgag tgggttcggg
     7021 caacagcatg agatacttgc attgtcattt ttatcgtgtg gattttaacc agggtttata
     7081 aggctgcgca actgcgcggc ctttttcgtt ttgcgggctg cggttctcct cttttgattc
     7141 tccttgtggc cggaccgtgg cccgcaactg ttgaggaaaa tcccggaaag gggaggaata
     7201 atggcattta aacactatga cgtggtcagg gcggcgtcgc cgtcagacct tgcggaaaag
     7261 ctgacacaca aactgaaaga gggctggcag ccgtttggta gtccggtggc cataacccct
     7321 tataccctga tgcaggcgat tacagcagaa ggtgatgtgg tggtcagtgg tgcaactgag
     7381 ccggattggt actacgtcat cgtactggcc gggcagtcca atgccatggc ttacggtgaa
     7441 gggcttccgc tgccggattc atacgatgct ccggatccgc gcattaaaca gctggcgcgc
     7501 cgcagtacag tgacgccggg tggggctgcc tgcagatata acgatattat tccggccgac
     7561 cactgcctgc atgatgtgca ggatatgagt acgctgaatc atccgaaggc agacctgagc
     7621 aaagggcagt acggctgtgt cggccagggg ttacatattg ccaaaaaact gctcccgtat
     7681 atcccgaata acgcggggat cctgctggta ccatgctgtc gtggtggttc ggcattcacc
     7741 cagggcgc
//
LOCUS       DAZKIV010000180         7671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZKIV010000180 DAZKIV010000000
VERSION     DAZKIV010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.180"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..239)
                     /locus_tag="ACHOT6_004160"
     CDS             complement(<1..239)
                     /locus_tag="ACHOT6_004160"
                     /inference="COORDINATES: protein motif:HMM:NF027767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949279.1"
                     /translation="MIEDGSYVFDSSFLPANLSSPIVIQDGLVTYICDQKFSEVLDGH
                     WLINIDGTYSIRKITKLPKGMIKITTTENSFECAFS"
     gene            complement(309..548)
                     /locus_tag="ACHOT6_004161"
     CDS             complement(309..548)
                     /locus_tag="ACHOT6_004161"
                     /inference="COORDINATES: protein motif:HMM:NF018697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9949280.1"
                     /translation="MSTSKYPSEIKINPNKGGKAAIERLVEAYGFTTRQALADHLEVS
                     KSTLANRYLRDTFPADWIIQCALETGTSLKWLTTG"
     gene            644..865
                     /locus_tag="ACHOT6_004162"
     CDS             644..865
                     /locus_tag="ACHOT6_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HFP9949281.1"
                     /translation="MTPNISITLNTPHVTIERYSELTGLSIDTINDMLADGRIPRHRL
                     RKDKKREKVMINLAALTVDALTDCNVVFN"
     gene            898..1407
                     /locus_tag="ACHOT6_004163"
     CDS             898..1407
                     /locus_tag="ACHOT6_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HFP9949282.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PSQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            1415..1615
                     /locus_tag="ACHOT6_004164"
     CDS             1415..1615
                     /locus_tag="ACHOT6_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage filamentation protein Fil family protein"
                     /protein_id="HFP9949283.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            1579..1920
                     /locus_tag="ACHOT6_004165"
     CDS             1579..1920
                     /locus_tag="ACHOT6_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HFP9949284.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            1988..2221
                     /locus_tag="ACHOT6_004166"
     CDS             1988..2221
                     /locus_tag="ACHOT6_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFP9949285.1"
                     /translation="MRNIETLTTKTGPDDAGLNLLLTEARLEERRARAEAMAARLDSL
                     ACHITSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            2221..2448
                     /locus_tag="ACHOT6_004167"
     CDS             2221..2448
                     /locus_tag="ACHOT6_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFP9949286.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCVECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            2445..3302
                     /locus_tag="ACHOT6_004168"
     CDS             2445..3302
                     /locus_tag="ACHOT6_004168"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0032775 - obsolete DNA methylation on
                     adenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFP9949287.1"
                     /translation="MSTILKWAGNKTAIMSELKKYLPAGPRLVEPFAGSCAVMMATDY
                     PSYLVADINPDLINLYKKIAADCEAFISRAKVLFEEANSELAYYNIRQEFNYSTEITD
                     FMKAIYFLYLNRHGYRGLCRYNKSGYFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASILEHRSSEGHPVIV
                     SNNDTSLIRSLYRNFTHHYIKAKRSIGVSAGEGKSATEIIAVSGPSCWLGFDPSRGVD
                     SSAVYGVCV"
     gene            3299..5713
                     /locus_tag="ACHOT6_004169"
     CDS             3299..5713
                     /locus_tag="ACHOT6_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFP9949288.1"
                     /translation="MSHDDMNNCSGFNEAAAAFSWNGSKKAINPYLDPAEVAPESALS
                     NLITLYAADNEQEQLRREALSEQVWERYFFNESRDPVQREMEQDKLISRAKLAHEQQR
                     FNPDMIILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLDCVRD
                     SQLSVSFRFMASHQGLEGLLILPEMSQDQVKRLSTLVAAHMSMCLEDACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEKLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRSDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAELRWYGVRVAEPHHDGTVHWHLMCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQQGKDKSADGDSRTDITRMNDKELHDYLHSMSKKERRELAARLRQVKPKRRK
                     DYKQRITDHQRQQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLKEDD
                     KWRNLY"
     gene            5866..6054
                     /locus_tag="ACHOT6_004170"
     CDS             5866..6054
                     /locus_tag="ACHOT6_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949289.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYNRSMDVIS"
     gene            6065..6298
                     /locus_tag="ACHOT6_004171"
     CDS             6065..6298
                     /locus_tag="ACHOT6_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HFP9949290.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRVRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(6491..6826)
                     /locus_tag="ACHOT6_004172"
     CDS             complement(6491..6826)
                     /locus_tag="ACHOT6_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949291.1"
                     /translation="MNNIPPIPQLGIYVSKIDPTLRITVTDVDIVDGEDDSPDDELFY
                     LVHWIEGEDESDMTAMGFELDPVEWQAFVESEQLVFERDPYMDSIPENSNLAKIRDFL
                     MKTKQNDHS"
     gene            7472..>7671
                     /locus_tag="ACHOT6_004173"
     CDS             7472..>7671
                     /locus_tag="ACHOT6_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010835378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HFP9949292.1"
                     /translation="MAPLSILERLQNAANRQDLASILNLKTAFLTDVIYRLKAETQYT
                     QFTIPKKNGAPRVISAPTTKLK"
BASE COUNT         2037 a         1728 c         1911 g         1995 t
ORIGIN      
        1 gaaaatgcac attcaaagct attctctgta gttgtaattt taatcatacc ttttggaagc
       61 tttgtgattt ttcgaatgga ataggttccg tcgatgttga ttaaccagtg tccatcaagt
      121 acttcagaaa atttttgatc acaaatgtat gtgacaagcc catcctgaat aacaattggt
      181 gatgaaagat ttgcaggaag aaatgatgaa tcgaatacat atgaaccatc ttcaatcatt
      241 ttgcctgcgg taaggcgaaa cttgggaagc tcttctgtgg ctactgtcag cgagctttgc
      301 ttaagtcctt acccggtggt taaccatttc agtgaggttc ctgtttcaag agcacattgg
      361 attatccagt ctgcaggaaa cgtatcccgc aaatacctgt tcgccagagt gctttttgat
      421 acttccaagt gatcagctaa agcctgtcgt gtcgtaaagc cataagcttc gactaatcgc
      481 tcaatcgcag ccttaccacc tttattggga tttattttga tctcacttgg gtactttgat
      541 gttgacatat ctcttttgcg atcctggtat cagttttgtc cccatttggg tacttgtcac
      601 gattactaca ggctcaccac aagccaatag gagatgttgc atcatgaccc ctaacatttc
      661 aataactctg aatacgccac atgtcacaat tgagcgttat agcgaactta ctggtctttc
      721 aatcgacaca attaacgata tgctggctga cggtcgcatc cctcggcatc gccttcggaa
      781 agacaagaaa agagaaaagg tgatgatcaa ccttgctgct cttaccgttg atgcacttac
      841 tgattgcaat gttgtattca actagttcca ttttgggatg catcaggggt gtcgaccatg
      901 tttgattacc aagtttccaa acatccacat tttgatgaag cctgtcgtgc attcgcattg
      961 cgccacaacc tggtgcaact ggcagaacgt gctggcatga atgtgcagat tctgcggaac
     1021 aagctgaacc catctcaacc tcatttatta accgcaccag aaatctggct gcttaccgat
     1081 ctgacagaag attcaacgct ggtagatggt tttctggcac agattcactg cctgccatgt
     1141 gtaccgatta atgaggttgc aaaagagaaa ctgccgcatt acgtcatgag cgcaactgca
     1201 gagatcggac gtgttgcagc aggtgcggta tctggtgatg taaaaactag tgccggtcgt
     1261 cgtgatgcta tcagcagcat taactctgta acacgactga tggcgctggc tgctgtttca
     1321 ttgcaggccc gtttacaggc taatcctgcg atggcgagtg cagttgatac cgtgactggc
     1381 ctcggtgctt cattcggttt gctgtgaggt gcttatgctg acgaaagaac catcatttgc
     1441 atcgctgctg gtaaaacaaa gcccggcaat gcactacggt cacggctgga tcatgggtga
     1501 ggatggtaaa cgctggcatc cgtgccgttc acaagatgaa ttgctggcag aactatctac
     1561 gaaaaaacgg gggaacaaat ggctattgaa ggcgctgcgg cgactgttcc attaagcccc
     1621 ggtgaacgcc tgaatggact taatcacata gcggaattaa gggcgaaagt ttttggtttg
     1681 aatattgagt cagagcttga gcggtttatt aaagatatgc gtgatccacg ggatatcaat
     1741 aatgaacaaa ataaacgggc actggctgcc atattcttta tggcaaaaat tccagctgaa
     1801 cgtcatagca tcagcattaa tgagctgacc actgacgaaa agcgggagtt gattaaagca
     1861 atgaatcatt ttcgtgcagt ggtgagctta tttcccagac ggctaaccat gccgaattaa
     1921 ccaactaatg aaattaatgg cgtaaacccg ccgggcatcc ctttatctaa attcaggaga
     1981 attgcttatg cgtaatattg aaaccctcac gaccaaaacc ggaccggatg atgcaggtct
     2041 taatctttta ctgacagagg ctcgtctgga agaacgccgg gcaagggctg aagcaatggc
     2101 tgctcgcctc gatagcctgg cgtgtcatat cacctcccgc cagctaaacc acgtcgaagc
     2161 ggcagaactg ctgcgtgtga ctgctgaagc aatccagaac gaagcgcagg agatccactg
     2221 atggctgatg caatggatct cgtacagcag cgcgttgaag aagaacgcca acgccatatc
     2281 cgtgctgccc gtgccaaaac gccgggcgtg tcccgcgtgc tttgcgttga gtgtgaagcg
     2341 ccaattccgc cagcacgccg ccgtgccatt cctggagtgc agctttgcat tacctgtcag
     2401 gaaatcgcag agctgaaagg caaacattac aacggaggtg ctgtatgagc accatcctga
     2461 aatgggcggg taataaaacc gccattatgt ccgaactgaa aaaatacctt cctgctggcc
     2521 cgcgactggt tgaacctttc gcgggttctt gtgctgtgat gatggcgacg gattacccca
     2581 gctatctggt tgcggatatt aatcctgatt taatcaacct ctataaaaag attgccgctg
     2641 attgcgaagc gtttatatct cgtgccaaag ttttatttga ggaagcaaac agtgagctgg
     2701 cttattacaa cataagacag gagtttaatt actcaactga aattactgat ttcatgaagg
     2761 cgatatattt cctgtatctc aatcgtcacg gttaccgtgg tttatgtcgc tataacaaga
     2821 gcgggtattt caacattccc tacggtaatt ataaaaatcc gtatttccct gaaaaagaaa
     2881 ttcgcgcatt tgcagagaaa gcccagcggg caacgtttat ctgcgcgagc tttgatgaaa
     2941 cgctggcgat gctgcaggtg ggggatgtgg tgtattgcga tccgccgtat gacggtacgt
     3001 tttccggcta tcacactgat ggtttcactg aagatgacca gtatcacctg gcatccattc
     3061 ttgaacatcg gtcatcagaa ggacatccgg tcattgtttc taacaatgac acatccctga
     3121 ttcgttcgct gtatcgcaat tttactcacc actatatcaa ggcaaaacgc agcatcggcg
     3181 tgtcagctgg cgagggtaaa tctgcaacag aaatcattgc tgtttccggg ccgagctgct
     3241 ggttgggatt tgatccttcg cgtggcgtgg atagttctgc cgtgtacgga gtgtgtgtat
     3301 gagtcatgac gatatgaaca attgcagcgg ctttaacgag gccgccgcag cattttcatg
     3361 gaacggctcg aaaaaggcca ttaaccctta tctggacccg gcggaagttg cgccggagtc
     3421 tgcactttca aacctgatca ctctgtacgc tgccgataac gagcaggaac aactgcgccg
     3481 cgaggcactg agtgagcagg tctgggagcg ttatttcttt aatgaatccc gtgatcctgt
     3541 ccagcgcgaa atggagcagg ataagctcat tagccgggca aagctggcgc atgaacagca
     3601 gcgttttaat ccggacatga tcattctggc ggacgtaaac gcccagcctt cccatatcag
     3661 caagccgctg atgcaacgta ttgaatactt cagcagcctg ggcaggccaa aggcttattc
     3721 ccgctatttg cgtgagacga ttaagccatg tctggagcga ctggattgtg tacgtgacag
     3781 tcagctatct gtttctttcc gttttatggc aagccatcaa gggctggagg gtctgctgat
     3841 cctgcctgaa atgagccagg atcaggtgaa acgcctgtcc accctggtag cagcgcatat
     3901 gagcatgtgt cttgaggacg cttgtggtga tttgtatgcc accgatgacg ttaagccaga
     3961 agaaatccgc aagacatggg aaaaggtggc agcggaaacc ctgcgtctgg atgttatccc
     4021 gcctgcgttt gagaaacttc gtcggaaaag aaaccgccgc aaaccagtgc cctatgaact
     4081 catcccgggt tcgctggcgc gtatgttgtg tgccgactgg tggtatcgga aattatggaa
     4141 gatgcgttgc gaatggcggg aagagcagtt gcgtgctgtc tgcctggtca gcaaaaaagc
     4201 atccccctat gtcagctatg aagccgtgat gcataaacgt gagcagcgcc gtaagtcgct
     4261 ggagtttttt cgttctcatg aactggtgaa cgaagacggc gacacgctgg atatggaaga
     4321 tgtggtaaac gccagcagca gcaacccggc gcatcgccgc aatgagatga tggcctgtgt
     4381 taaaggtctg gagcttatcg cggaaatgcg cagtgactgt gccgttttct acactatcac
     4441 ctgtccgtcg cgtttccatt ccacgctcaa taacggcaga ccaaacccga cctggacaaa
     4501 cgcgacggta agacaaagta gcgattatct ggtcggcatg tttgctgcat ttcgtaaggc
     4561 tatgcataaa gccgagttgc gctggtatgg cgtgcgggta gctgagccgc atcatgacgg
     4621 tactgtgcac tggcatctca tgtgtttcat gcgtaaaaaa gaccgccgcg ccatcactgc
     4681 attactgcgt aagtttgcca tccgtgaaga ccgcgaggag ctgggcaata acacgggacc
     4741 acgctttaag tctgagctta taaacccgcg caaaggtacg ccaacaagct atatcgcgaa
     4801 atacatcagt aagaacattg acgggcgtgg tctggctggg gagatcagca aggaaacggg
     4861 taaatccctg cgtgataacg ctgaatacgt taatgcctgg gcgtctctgc atcgtgttca
     4921 gcaattccgc ttctttggca ttccggggcg tcaggcttac cgtgaactgc gattgctggc
     4981 tggtcaggcg gcaaggcaac agggtgacaa aaaagcaggt gcgccggtac tggataaccc
     5041 gcgccttgat gccattctgg ctgctgctga tgctggttgt tttgccacct atatcatgaa
     5101 gcagggcggc gtactggttc cccgtaaata tcacctcatc agaaccgctt atgaaatcaa
     5161 cgaagagcca accgcctatg gcgatcacgg tattcgtatt tatggcatct ggtcacccat
     5221 tgcagagggc aagatctgca ctcatgcggt gaagtggaaa atggttcgta aggccgttga
     5281 cgttcaggag gcggcagccg accagggcgc ttgcgcccct tggactcgtg gcaataactg
     5341 tccccttgct gaaaatttga accaacaagg gaaagacaaa tcagctgatg gggactccag
     5401 aacggatatt acccgtatga atgacaagga gttgcacgat tacctgcaca gtatgagcaa
     5461 aaaagagcgc cgggaactgg ctgcaaggtt acgccaggtg aaaccgaaac ggcgtaaaga
     5521 ctacaaacag cgaattacag accatcagcg acagcagctc gtctatgaac tgaagtccag
     5581 gggatttgat ggcagcgaga aagaagtcga tttgctcctt cgcggcggca gtattccgtc
     5641 aggagcaggc ctgcgtatct tctatcggaa ccagcgtctg aaggaagatg ataagtggcg
     5701 gaacctatat taattacgcg ggttaacaat tcgtgctctt aataatacca ggcatatcag
     5761 gccgatgaac gtaaaaaaac gttttacatc agtaagatta ttatatactg taaatataaa
     5821 cagtggttat gtgtacagta ttgttttggt gtcataggag gaaagatgca ggactatttt
     5881 ttggagtctt tgaagctcca gcgcattgat ttttttctta agcttgtagc ggctagtgag
     5941 tgtagtgatg aagagaaggg gctggctctg cagtgggttt ctgaattgac tgatgaactc
     6001 atggcaaaaa tcagaagcca cgaatacaac cgctcaatgg atgtcatcag ctgaggtgac
     6061 ttttatgcgc attgaaataa tgatcgataa agagcagaag attagccagt ctaccctgga
     6121 cgcccttgaa tccgagcttt accgcaatct gcgccccctg tatcccaaaa cggtaattcg
     6181 cgttcgcaaa ggtagctcta acggtgtgga actaaccgga ctgcaactgg atgaagaaag
     6241 aaaacaagtg atgaaaatta tgcagaaggt gtgggaggac gacagctggc tgcattaaga
     6301 aatgttgccc ccaggaggat tcattctgat gggggctagt ttgggcaatg agtgaaataa
     6361 ggcgtaaggt gggcggttat tttgataagt gatcgtccgc tttgtgtcag aagcagaagt
     6421 gggagtgtct gagactctct aaaagttgat gattcactta caaaaccatt ttcctgatgg
     6481 atgcttacac ttacgaatga tcattctgtt tagtcttcat gagaaaatcc cgaatctttg
     6541 ccaagtttga attttcgggg attgaatcca tgtacggatc acgctcaaac actaattgct
     6601 cagattcaac gaaagcctgc cactctactg ggtctagctc aaatcccatt gctgtcatat
     6661 cactttcatc ttccccctcg atccagtgga ctaaataaaa caattcatca tcaggagaat
     6721 catcctcacc atcaacaata tcaacatcgg ttacagtgat acgtagggtg ggatcgattt
     6781 ttgagacata aattcctaac tgtggtatag ggggaatatt gttcatggaa actcctttta
     6841 ttggtgtttg ctatttgaat aaaaatacgc caaacagagt gttaactgtg aatatggaaa
     6901 gaatcgttgt aagtaccccg cttaggtgtc ccattctatt ttagaggttc tcgtgcatat
     6961 ggattatgtt atctgaaagt taacttccgc ttcccgttca cagcgaacat tcatctttgt
     7021 aaacccgtat gatccgtttt tcaagtggcc attcagatac ggatttttac ttctttgaca
     7081 gtgcatgact atgctgcatg aaatcgcatg atcgattgag gatcgtcttt gctcagatcc
     7141 gccagaactg gcgggctttt gctcatgtca tgcatgtgca tgaaaaccac tgcataaagc
     7201 gggcaggcgt ggcggggata cgagcgcgcg caatgctatg atctggctct ttcaaaactg
     7261 ttaacggttc ttcacattct taaatgatca tggtagctta aacatgcaca tgtaggcaga
     7321 tttgttggtt gtggtatcgc aatcagtgat ctaaatgtta gatggattcg ccttctgaca
     7381 tccttgtagc cattcttggg ctgtcctatg ctttgcgtaa gagtgcctac atgtgcctac
     7441 cctaactgat aatcaaggtt gatttttaga tatggcaccc ctatcaattt tagaacgttt
     7501 acaaaacgct gctaacaggc aagatttggc tagtatcttg aatcttaaaa ctgcttttct
     7561 tacggatgtt atttatcgtt taaaagctga aacgcagtat acacaattta ctattcctaa
     7621 aaaaaacgga gcgccgagag tcatatcagc acctaccact aagctcaaag a
//
LOCUS       DAZKIV010000181         7663 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZKIV010000181 DAZKIV010000000
VERSION     DAZKIV010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.181"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..798
                     /gene="csgD"
                     /locus_tag="ACHOT6_004174"
     CDS             148..798
                     /gene="csgD"
                     /locus_tag="ACHOT6_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFP9949293.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            803..1192
                     /gene="csgE"
                     /locus_tag="ACHOT6_004175"
     CDS             803..1192
                     /gene="csgE"
                     /locus_tag="ACHOT6_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFP9949294.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1217..1633
                     /gene="csgF"
                     /locus_tag="ACHOT6_004176"
     CDS             1217..1633
                     /gene="csgF"
                     /locus_tag="ACHOT6_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFP9949295.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1660..2493
                     /gene="csgG"
                     /locus_tag="ACHOT6_004177"
     CDS             1660..2493
                     /gene="csgG"
                     /locus_tag="ACHOT6_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFP9949296.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2557..3048)
                     /gene="ycdZ"
                     /locus_tag="ACHOT6_004178"
     CDS             complement(2557..3048)
                     /gene="ycdZ"
                     /locus_tag="ACHOT6_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFP9949297.1"
                     /translation="MNILLSIAITTGIFSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLIFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3150..3704)
                     /gene="ycdY"
                     /locus_tag="ACHOT6_004179"
     CDS             complement(3150..3704)
                     /gene="ycdY"
                     /locus_tag="ACHOT6_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFP9949298.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGELAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3728..4465)
                     /gene="ycdX"
                     /locus_tag="ACHOT6_004180"
     CDS             complement(3728..4465)
                     /gene="ycdX"
                     /locus_tag="ACHOT6_004180"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFP9949299.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4520..5458)
                     /gene="ghrA"
                     /locus_tag="ACHOT6_004181"
     CDS             complement(4520..5458)
                     /gene="ghrA"
                     /locus_tag="ACHOT6_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFP9949300.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLYWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5692..5779
                     /locus_tag="ACHOT6_004182"
     tRNA            5692..5779
                     /locus_tag="ACHOT6_004182"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5726..5728,aa:Ser,seq:gga)
     gene            5824..5960
                     /locus_tag="ACHOT6_004183"
     ncRNA           5824..5960
                     /ncRNA_class="other"
                     /locus_tag="ACHOT6_004183"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(6153..7139)
                     /gene="ycdU"
                     /locus_tag="ACHOT6_004184"
     CDS             complement(6153..7139)
                     /gene="ycdU"
                     /locus_tag="ACHOT6_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFP9949301.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(7136..>7663)
                     /locus_tag="ACHOT6_004185"
     CDS             complement(7136..>7663)
                     /locus_tag="ACHOT6_004185"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFP9949302.1"
                     /translation="AVKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQAALNEF
                     ATLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEV
                     APSIIKENLSLHLTHTVKWTESFDTFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQR
                     VMHCRKAFSDAKVFK"
BASE COUNT         2162 a         1887 c         1717 g         1897 t
ORIGIN      
        1 agatgtaatc cattagtttt atattttacc catttagggc tgatttatta ctacacacag
       61 cagtgcaaca tctgtcagta cttctggtgc ttctatttta gaggcagctg tcaggtgtgc
      121 gatcaataaa aaaagcgggg tttcatcatg tttaatgaag tccatagtat tcatggtcat
      181 acattattgt tgatcactaa accttctttg caggcgacgg ctctcttgca gcaccttaaa
      241 caatcgctgg caattacagg aaaattacat aatattcaac gttctctgga cgatatatct
      301 tcaggctcta ttattcttct ggatatgatg gaagcagata aaaagcttat ccattattgg
      361 caggatactt tgagcaggaa aaacaacaat atcaaaatat tgttgctaaa tacgcctgaa
      421 gattacccgt accgcgacat tgaaaactgg cctcatatca acggcgtttt ttatgccatg
      481 gaggatcaag aacgtgttgt caatgggttg caaggcgtcc tgcgcggcga atgctacttt
      541 acgcaaaagc ttgccagcta cttgattacg cattcaggta actatcgtta taacagcacg
      601 gaatcagccc tccttactca tcgggaaaaa gagatcctga ataagctgcg tatcggcgcg
      661 tctaataacg agatcgctcg ttcgttgttc atcagcgaaa atacggttaa aacgcatctt
      721 tataatcttt tcaagaagat agccgtaaaa aaccggacac aagcggtttc ctgggcaaac
      781 gataacctca ggcgataaag ccatgaaacg ttatttacgc tggattgtgg cggcagaatt
      841 tctgttcgcc gcagggaatc ttcacgccgt tgaggtagaa gtcccgggat tgctaactga
      901 ccatactgtt tcatctattg gccatgattt ttaccgagcc tttagtgata aatgggaaag
      961 tgactatacg ggtaacttaa cgattaatga aaggcccagt gcacgatggg gaagctggat
     1021 cacaataacg gtcaatcagg atgttatttt ccagactttt ttatttccgt tgaaaagaga
     1081 cttcgagaaa actgtcgtct ttgcactgat tcaaactgaa gaagcactaa atcgtcgcca
     1141 gataaatcag gcgttattaa gtacgggcga tttggcgcat gatgaattct aaataaaaaa
     1201 ttgttcggag gctgcaatgc gtgtcaaaca tgcagtagtt ctactcatgc ttatttcgcc
     1261 attaagttgg gctggaacca tgactttcca gttccgtaat ccaaactttg gtggtaaccc
     1321 aaataatggc gcttttttat taaatagcgc tcaggcccaa aactcttata aagatccgag
     1381 ctataacgat gactttggta ttgaaacacc ctcagcgtta gataacttta ctcaggccat
     1441 ccagtcacaa attttaggtg ggctactgtc gaatattaat accggtaaac cgggccgcat
     1501 ggtgaccaac gattatattg ttgatattgc taaccgcgat ggtcaattgc agttgaacgt
     1561 gacagatcgt aaaaccggac aaacctcgac catccaggtt tcgggtttac aaaataactc
     1621 aaccgatttt taagccccag cttcataagg aaaataatca tgcagcgctt atttcttttg
     1681 gttgccgtca tgttactgag cggatgctta accgccccgc ctaaagaagc cgccagaccg
     1741 acattaatgc ctcgagctca gagctacaaa gatttgaccc atctgccagc gccgacgggt
     1801 aagatctttg tttcggtata caacattcag gacgaaaccg ggcaatttaa accctacccg
     1861 gcaagtaact tctccactgc tgttccgcaa agcgccacgg caatgctggt cacggcattg
     1921 aaagattctc gctggtttat accgctggag cgccagggct tacaaaacct gcttaacgag
     1981 cgcaagatta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa ccgaatcccg
     2041 ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg ttatgaaagc
     2101 aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga cacgcaatac
     2161 cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg tgagatcctc
     2221 tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg ggttttccgc
     2281 tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa cgaacccgtt
     2341 atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa tgatggtatc
     2401 gaccgtggtc tgtgggattt gcagaacaaa gcagaacggc agaacgacat tctggtgaaa
     2461 taccgccata tgtcggttcc accggaatcc tgacagataa aaaagccgga attatcatcc
     2521 ggcattttac tgtttaagag aaagcgtgag tgcccctcac gctttatttt tgatttcctg
     2581 ctcacggtgc gccgtctttg cactacgcgc cgccagccac aggccgctgt ttttcattgc
     2641 gtaaccaaat atcagcccaa gcgccagcga aggtaacacc agtttccagt caccctgccc
     2701 ggcaaacgtc gcgcatgcgc ctataaacgt tcccggaaca aatgaaagca gcagctgttt
     2761 ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga gaatttccag
     2821 atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc cactaagcag
     2881 ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa agtaggccgt
     2941 acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc atccccagat
     3001 accggagaaa atgcctgttg tgattgcaat agagagaagt atgttcatgc cgcgcatgat
     3061 agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa caaattatct
     3121 gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc cagctcgtcc
     3181 cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa aggcgtggtt
     3241 gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata ctcactgaac
     3301 agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca ggaagccgca
     3361 agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtggcatccc tcgttcggaa
     3421 agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg atatggcggc
     3481 acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg cgccatatca
     3541 cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt cgcagccagt
     3601 tccccctcac gaatcagggt aaacagcggc accagtaaag gatcttgcgg ttggcggtaa
     3661 tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat atttccatta
     3721 ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat tcaaggaagt
     3781 tcagtaagcg gcgcggagaa acattcaaaa tgcgctctgg cggaaaatct accgcgtcga
     3841 ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa tccgagccta
     3901 atgccaccca acctccagca tcacgtaccg ctgcggctac cgcacgacag ttgtcttcac
     3961 tgcccttacg tgagtgtaaa aatgaagaat tattgatttc cagcgccacc tgatgtttcg
     4021 cggctgcttc agcaacggct ttcacatcta tttcatattt gggatttccg ggatggctta
     4081 ttatatgcac attgccgctg gcgatagtgg cgatcatcgc ttgtgtattg gtcgctttgt
     4141 catgtggtgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc gagtcaaaca
     4201 ttttaccgct gcaatcaatt tcaccatcaa catttttaat gttagcttcg atgccgcgca
     4261 ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag tgccagtgat
     4321 gcggagcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta atgccctttt
     4381 gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg gcaacggtat
     4441 gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg ccaaggatag
     4501 caggaatcct gatgctttat tagtagccgc gtgcgcggtc gacttgcccg cagaccctct
     4561 cccctttttc gagctgggca atggtgcgag aaatgtactc cacagcttca gcgggacggg
     4621 taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc ggactttcag
     4681 gcggtaaggg ttcacgatta aaaacatcca acattgcgcc tttaactttg ccgctatcca
     4741 gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg aggagatacg
     4801 cgccatccgg taatttttcg agtaattgtt gattaataat gccgacggtt tcaggggtat
     4861 tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt tcttcccgtc
     4921 cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa cgcagcggaa
     4981 agcgccaggt ttgcagactc tgagcaactt tactgcccag tacgcctgcg cccaaaatgc
     5041 cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa ctattttgct
     5101 ggatgcgata atcgtcaaaa cgtcgaaacc aatacagcac ctgactgaca gcatattcct
     5161 gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa gggttcagca
     5221 tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg agtgcgaaca
     5281 ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact aaagcataat
     5341 cagcagagtc attatctccg cttttccatg ctctgactct tgcctgagga atagctttgc
     5401 gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg atatccatac
     5461 tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat ctacaaaggc
     5521 caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt attaaaacca
     5581 cacattgatt gaagtttgaa taaacgcgcg attttttcaa aaagtttgtt gacctcaggt
     5641 cgtgatttcc ctaaattagc gcccgttcca gcaagacagg aacgacaatt tggtgaggtg
     5701 tccgagtggc tgaaggagca cgcctggaaa gtgtgtatac ggcaacgtat cgagggttcg
     5761 aatccccccc tcaccgccat atttaaagaa gagctcgtac gaaagtacgg gctttttttt
     5821 cgtatattgc acacaccggg gggatgagaa gccccgaccg gggttcgaca actggcgaca
     5881 gccagttgga cagaccgtga acgcagtgag cgggctgccc gcagggcgag cgaagcgagt
     5941 caatcccccc ctcaccgcca tatttaaaga agagcccgta cgaaagtacg ggcttttttt
     6001 tcgtatattg cacacaccgg gtgcccccgc ccacgtctgt tagggcgagg ggaaattgtg
     6061 cacatcaact gtctggttac tcagtcaaca atcaaaatgc gcaagatagt agagaacata
     6121 tatgcgttat ctggtcactt cctgtaactt cctcacttat taaaatgccc ccaacggaaa
     6181 tacatgtcac atgccatcat aaaatcgtta tgccagagga atcgacaatg gaaaaaatag
     6241 taaattaagc acggtacata gcagagtaaa aaataggctt caaaccaaat ccgccattcc
     6301 acaagcgttg ccaataccga caccagcgta atccacatca tttcaataac cagcaatatg
     6361 aataaccgaa gccaaagcgg cttggcaaaa aaggtcgaca acgcattttc actggagaaa
     6421 aacagatcga tttcctgcaa aaaaagcctg cccagaaaag cgtaccgctt ggagaagcgc
     6481 agaaaaaaca gattaatcgc caacactacg ccacaaagaa tgagcaatgc aattatcgat
     6541 ttaaacgtta accactgccc ctctgtcaca aaagaatcac tgttacgcaa cgggctgata
     6601 gtaagcaggt taacaaccgt tgagatgcac accgaaagca gcacatagcg agtcagactt
     6661 ttatatttta cttttttcgc tactgaaata gcgacctgct tgacataact ggtgcggatc
     6721 atccgaaaca gcagaaagat gaccaacggc ccaagtaacg aaatcaggaa cagcgcatat
     6781 aacgagggta gaagctggta tagcccccaa ctcattgcac tcaacatgat taacgacaga
     6841 aatcctgcca ccagaggttg tgtaagaaat aattctattt tgtggttctg ctttttctta
     6901 aaccacgaaa aatcgatatt cttcgtgacg catttataag cccagtacgc cttgagatca
     6961 aaaataaaat tatacagcac cagggccaga atcccactaa ctacaccaat atcctctgat
     7021 ggaaaagtaa accctgcatt cttccaataa aagcctatca gaataagtcc aatagcataa
     7081 atgtatatca gatttcgttt atcctgaaag cgaataaaag ttaaataatc aggaatcatt
     7141 tgaatacctt tgcatcacta aatgctttac gacaatgcat aactcgttgc tgatccacgc
     7201 cagcaaaatc atttaacata ttccccaaat agggcttatt acttatttca aggaaagcta
     7261 ccggggttgg catattcaga aatgtatcga aagattcagt ccatttcacc gtatgagtta
     7321 agtgtaatga gagattttct ttgataattg acggagcgac ctcgcttttt gctgttacgt
     7381 tcatcagaac ctgatgctct ggcgaagcga tatccagtcc ggcaagataa tcgcgcattg
     7441 cctgaacgcc gtcttccatc aaacgcgtgt gccaggcacc gctcacaccg agtttaaccg
     7501 gttcgtatcc agcggccatc agcagcgtgg caaattcatt caacgcagcc tgcgttcccc
     7561 caataacctg ctggcgcggc gtgttatcac agctaatatc cagcgcaatg cctgattccg
     7621 taatcatcgt ctgcagttgt tcgcggttga tgcctttaac cgc
//
LOCUS       DAZKIV010000182         7602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZKIV010000182 DAZKIV010000000
VERSION     DAZKIV010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.182"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..482)
                     /locus_tag="ACHOT6_004186"
     CDS             complement(<1..482)
                     /locus_tag="ACHOT6_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9949303.1"
                     /translation="MQDIHEESLNESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRQYQAYPIDGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     A"
     gene            complement(482..823)
                     /locus_tag="ACHOT6_004187"
     CDS             complement(482..823)
                     /locus_tag="ACHOT6_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949304.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLDEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            complement(816..4058)
                     /locus_tag="ACHOT6_004188"
     CDS             complement(816..4058)
                     /locus_tag="ACHOT6_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9949305.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFASSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VTKTLSGMLNGVAGQIENVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQYVLANP
                     GWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLAQMQEKAQSIQDVLAGLEDRR
                     VALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRLPQATLD
                     DKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYISNALEAW
                     RNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALVGQNTELAKLKYQTAQGELKT
                     LTEMQKQELLRNAALIDQQKIREQLRSREETLKNENAAARASNDAELLGYGQGERARE
                     RMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERLKDQAAFYAES
                     DAQRADWQKGLQEGLSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNKVEWRNW
                     ASSVLQEISKVLMNAAIVNGIKTAANGMSGAGGFIGSIGDWLGGAVANAKGGVYTSAN
                     LSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMNGSAGLV
                     YSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            complement(4110..4319)
                     /locus_tag="ACHOT6_004189"
     CDS             complement(4110..4319)
                     /locus_tag="ACHOT6_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFP9949306.1"
                     /translation="MWMEFDRVSPLGDERGDIRNAQIVKAVFGAQGMNVALKDAMLCW
                     GEDEDKPEVDPFAALEDALSLAAMS"
     gene            complement(4415..4789)
                     /locus_tag="ACHOT6_004190"
     CDS             complement(4415..4789)
                     /locus_tag="ACHOT6_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFP9949307.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            complement(4795..5511)
                     /locus_tag="ACHOT6_004191"
     CDS             complement(4795..5511)
                     /locus_tag="ACHOT6_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFP9949308.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMEKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDTVYAFKVQ
                     FPSGKGFKFLAEVRQHTWSSGTNGVVAATFSLRLKGKPVSYVVPLAFVKNLEKTLTVN
                     TGALLTMSVSVNGGTPPYKHAWKKDGQPVEGQTTDTFSKANTQSGDKGAYTCEVTDSA
                     EQPQSITSDACTVTVNGAGG"
     gene            complement(5570..5914)
                     /locus_tag="ACHOT6_004192"
     CDS             complement(5570..5914)
                     /locus_tag="ACHOT6_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HFP9949309.1"
                     /translation="MTEADLYPHLAHLAGGQVYPYVVPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(5911..6357)
                     /locus_tag="ACHOT6_004193"
     CDS             complement(5911..6357)
                     /locus_tag="ACHOT6_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFP9949310.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(6354..6704)
                     /locus_tag="ACHOT6_004194"
     CDS             complement(6354..6704)
                     /locus_tag="ACHOT6_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFP9949311.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDAT
                     GGRLEILCRLGGEK"
     gene            complement(6714..7040)
                     /locus_tag="ACHOT6_004195"
     CDS             complement(6714..7040)
                     /locus_tag="ACHOT6_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFP9949312.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVIREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(7037..>7602)
                     /locus_tag="ACHOT6_004196"
     CDS             complement(7037..>7602)
                     /locus_tag="ACHOT6_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9949313.1"
                     /translation="RPMLEQMVKEAVSHIPVPRDGRDYDPDVLQKAVLDAVSALPAPQ
                     DGRDATALEILPAIDDQKSFPRGTYATHQGGLWRAYEKTHGMRGWECLVDGVADIDVS
                     MTGERLFSVVVRQSSGQCTEKTFSLPVMLYRGVFRAGETYHPGDTVTWGGSLWHCNSM
                     TGDKPGEAHSSGWTLAAKRGRDAGGGK"
BASE COUNT         1538 a         2578 c         1786 g         1700 t
ORIGIN      
        1 gccccgtccg tctccgtcgg tgtggccagc acaaacgagg ctgtcatggc cgtcagctct
       61 gacatctgct ccaccaccca gcggtcgctc agctcctgct ccgggtccgc ttccggattg
      121 cccgccacaa aattcaccgc atccagaaaa cgggcataca cccggcggcg gaccaccgtg
      181 gcccccacca ggctctgcag gtcctccgcc atcccggtaa ccagaccaaa cagattggac
      241 accgtcagcg acggtctggc actgctgccc ttcccgttca tctcaaagcc actgccgtca
      301 atcgggtatg cctgatattg ccgcccctgc caggtcaccg gctccccttt ttcattcagc
      361 tcattgcaga aaaaataccg ctcaccaccc tgtaccgtca ggtcgatttc ccagagtacc
      421 acccgcggtg actgctctga cttaaccgac tcgttcaggc tttcttcgtg aatatcctgc
      481 atcagttcac cacctgctct atcgtgcaac tgaaatcact gtaccgggca ttatccgtga
      541 cactccactc ccggcacacc accctcaccg tccggttatg tttcggcggt cgccacaaaa
      601 aggcacggta accaccatgc caggataaaa attcatccag ccagcgccgg gttgactcat
      661 ccgtcacccg gaacaccgcc tgaaacgtct tcagttgagg attcagccct gtggggcggc
      721 gctgttcata accgtcacca aaccgcaccc tcaccaccga cggcttctca ctcacctgca
      781 tcccttcacg cgggaccaga tgcagcgttt ttatctcagc cactcagcat tcctccgtca
      841 cgtcgcatgg acagcatcac cgcctgcacc cgctggtcaa tcagctgcac aagactgccc
      901 gcagcttccg gccctatctg gccattagtc ccgtcattct gaatggcgat atggtagacc
      961 ggggaataca ccagacccgc actgccgttc atactgccca ccgctcgcac acccagcgag
     1021 ccatccgccg cccgggtcag gggcataatg gcttcaggac cggcctcccc catcagtccc
     1081 gccccttttg caaaggcaaa gtacgtgggc gtatccacaa tactgttgct gtacgcactc
     1141 aggtttgccg aggtatacac gccgcctttt gcattggcca ccgcaccgcc cagccagtca
     1201 ccaatgctgc caataaatcc tcccgcaccg gacataccgt ttgccgccgt cttaattccg
     1261 ttgacaatcg cggcattcat aagaactttt gagatttcct gcagtacgga tgaggcccag
     1321 ttgcgccatt ccactttgtt tccgttcagc atctccgtga tgttattcac catccctgag
     1381 ataccctccg tcgcaagctg tgctgcctgt gaggcgtaat cggatgcatt gtccacccag
     1441 ttactgagcc cctcctgcaa gcctttctgc cagtccgcac gctgcgcatc cgattcggca
     1501 taaaaggctg cctggtcctt aaggcgttcg ctcagatact gcgcgttctg tgcccgtgcc
     1561 tgtctgtaaa aatcctcact gatatccccg gtctgatact gagactgaag gtccgcatcc
     1621 ttctggcgga agctgtcgcg gatctgctgc aactcccgca tgcgttctct ggctcgttcc
     1681 ccctgcccgt accccagcag ttcagcatca ttcgacgcac gcgcagccgc attctcattc
     1741 ttcagtgtct cttcccggga tcgcaactgt tcccggattt tttgctggtc aatcagggcc
     1801 gcattacgca gcagctcctg cttctgcatc tccgtcaggg ttttcagttc gccctgcgca
     1861 gtctggtatt tcagcttcgc cagctctgta ttctgcccca ccagtgccag ttgctctttc
     1921 tgctgcttca gcagccggga aaaactgtct tccgcttttt ccgtctctga ttttccaccc
     1981 cgggatttgg gtttattcgc ctcgttattg cgccaggctt ccagggcatt actgatataa
     2041 cgctgtctcg cctcctgata cggatcaccc acaaaaccga ggtcatccgc cgcatacccc
     2101 agccggacac gctctttttc ttcccctttc agtctggaca gggccagctc acgctctgtt
     2161 tttgtcaggg cactctgctg tttatcatcc agagtggcct gtggcagccg taacggcaca
     2221 ttcaccagtc cctgccgctg ctgaagcagt tcattaccca gccccagcag acggttgaat
     2281 tccgtatgct gaccattcat aaccagcatg gactggtaca ccttattctg ctctgccgcc
     2341 tgctgacgaa ttaacgccac acgacggtct tccagcccgg caagcacatc ctgaatggac
     2401 tgcgcttttt cctgcatctg tgccagacgg gactgctcaa cggcaagctg ctctgttgcc
     2461 tgagcaagcc cttccgttac ggtcttcacc gaggtcagat ggtttatcat gaatccgtca
     2521 ccggtcgtcc agcccgggtt cgccagaaca tactgatatc ctgcgatttt ttcctgcagg
     2581 gatttcaccc gactggcctg ttcatcaatc agccggttct gctctgtcag cgccgcccgt
     2641 gttcgtcctt cattatctga ggcttcaggc aaagacattg acggcgtttt atgcgcgatt
     2701 tcatctatcg tcagtgcata ctggcgcgca gactccctgg cctgctcctg attctggtac
     2761 agcgtgtacc atgctgcagc ccccagcatc accagtccgg gtacgccacc aaccagcccc
     2821 agcgcaccgc tcatcagacg tgagcccacc gccgttgtac tgttcagcgc attctgggcg
     2881 gcgcttctgg cagcaatatt tctgttcagg cgttcctgtg tggccgccag acgggcctct
     2941 gcagcaatct gcatctccgt cccgcgggct gccgccacgg cctgctgagc acggtacacg
     3001 gctgcccttg cccgcgccgt ggcaatctgc gttcccctga actgtgcttc cgccagtgca
     3061 acttcattac gtgcagccgt cacaagtcct gccgtggcag acatcgctcc ggaggccata
     3121 ttgccaaagt agcgggcaac cccgacggca accagcgcgc ccacggctgt tgccacattc
     3181 tcaatctgtc cggcaacacc gttcagcatg ccggagagcg tttttgtcac cccgctggcc
     3241 tcattcgcac cgcccaccca ggccataaag gcgttttcca cctttgtgat actactggaa
     3301 accgtttccg gcatggccgc atattcatca cgtaatatcc ccagctggct gattaacgcg
     3361 gggaccactt tatccgctgt cagttttccg tcatccgcca ttgccttcag atctttacgg
     3421 gccacgccca tacccgcagc cagtgcacgt acgatccggt caccactttc attgaccgaa
     3481 ttaaactcct caccacgcaa tacaccctgc gccagcgcct gactgaactg ggtgatcacc
     3541 gagcccgcct ctgccgtact ggcaccggag attttcagcc ctgtcgaaat ggcctccgtc
     3601 accttcagca catcatcagc actgtaacca tattcacgca tcgaggcagc cgaacgggca
     3661 aacagggccg cattatccga aaatgcggtg cctgtccgct ggctgatatc catcagcact
     3721 ttctgtgatg acgcaaattc atccgatgac tgcgacgcct gtttcagacg ggcattcacg
     3781 gagctccatt catccgccag cgaaatcagg tgtccggtgg caaaggcacc ggcaaacgca
     3841 ccggtcattc cgacagccga agcgcggatt tccgtcaact ggctgtgcag ctcagccaga
     3901 gcccggcgct gctccctggc tgccgcagca gcctgacgtc cgccattctg cagggtccgg
     3961 taatattcac tgcccatacg ggacgcccgc tggatctccg actggaatga ctgtgaattt
     4021 gccgaaattt tgataatcag ttcacgtaac gtcgccattc acctttctcc gggcaaaaaa
     4081 aacctgccac agcaggtttt catcattatt tatgacattg ctgcaaggct cagcgcgtct
     4141 tccagcgccg caaacggatc cacctccggc ttatcctcat cctcgcccca gcagagcatg
     4201 gcgtccttca gtgcaacatt catcccctgt gccccgaaaa ccgctttcac gatctgtgca
     4261 ttacggatat ccccgcgctc atcacccagc ggggataccc tgtcgaactc catccacatc
     4321 atcgcctcgc tcgcactcag gctgtgccgc agttcggata aggtgcgccc cagacggagc
     4381 gcaagtcgca tcagaaagcg aatttccggg cgggctactt ttttctggcc gactctgcat
     4441 cagcgatcag ttccagtgcc tgacgcagca accgggcatg taccggacca tagacggcca
     4501 gcacctgctc acggtcgtcc ggagtgaaca cccgttgcag gtccgtatca cacaggacat
     4561 cgcagaacag cgtcacatcc gcttccaggt tacggcgggt tttcgccacc accgacaggg
     4621 tatcgtcatc ctctccatca ccattgagca cttcctgcca cagataccag gcctctgccg
     4681 aaggctcccg cagcaccacg ctgacatttc tccattccgg caccttcacc gttttatgac
     4741 ggaaccccga cagtctggcc agcgccagtg ttttcagatc ttttgccata agccttatcc
     4801 gcccgcacca ttaaccgtta ctgtacacgc atcagaggta atgctctgcg gctgttctgc
     4861 agaatccgtt acctcgcagg tataagcccc cttatcacct gactgcgtat tggctttact
     4921 gaaagtgtca gtagtctgtc cctctaccgg ctgaccatcc ttcttccagg cgtgtttata
     4981 aggcggcgtt cccccgttga cactgactga cattgtcagc agcgcaccgg tattcacggt
     5041 aagtgtcttc tccagatttt tcacaaacgc cagcggtacc acataggaca ccggtttacc
     5101 cttcaggcga agtgaaaacg ttgcagccac cacgccgttg gtaccggatg accaggtgtg
     5161 ctgacgcact tccgccagga acttaaagcc cttaccggac ggaaactgca ccttaaacgc
     5221 atacaccgtg tcattgtcat aggcatcacg cagggcgttc tgggcctgat tcagataaaa
     5281 attacccgac atggaaatct cggacgacgc ccccagaccg ttgatgttct cctgctctgt
     5341 ggagcagagc gtggtcacat caatatcctg tttctgaccg gcggtgaact ggacttcctt
     5401 gatggtgcag tccaggcgca gatattccgc cttctccatg gtttcagcag tcgccggggc
     5461 agatgaaatc atcacctgcg tcagctgtga acgttcatac aaagcagaca ttctgcctcc
     5521 tgataataaa aaacccgcac gcggcgggtt atgggttctg ttgaaaaaat tacaccgtga
     5581 cctgaaactc cagggttgca cggtaacagc ggttttccgg aatatagtcc tgcatttcac
     5641 tgacggatcc cggggccagc agcattatgg cttcacgggc gtcctgacgt atctgacgcg
     5701 cctgcgtcac agtcccggca taaacgtcta tctgcaccga cactgaggac tccgcctgcc
     5761 cgcccatcac gtccgccgac accgatgaaa tcaggctgaa aaccacccac ggaagcgcca
     5821 ccgacggcct gccatccagc agggggacca catacgggta cacctgcccg ccggcaagat
     5881 gcgccagatg aggatacaaa tccgcctccg tcatcgtctc agtacctcat caatggcccg
     5941 gttcatccga gcaatcgcca cctgtgccgc ctgttcactg cgcacatcaa atgccgggcg
     6001 cacaaacggg tgcggtggca tattcacggt ccccatttcc acaaaccgcc agtagaaagc
     6061 attgcgcggg ttatccgcct tcatggtgtt atcgctgtta ccggtgtccg gattaacacc
     6121 acggatatgg acaccggatt ccataccgcc atcgcgggag cgccgggaaa ggaccaccac
     6181 attgcggcgc agttttcccc tgcgcaccgg tgcccgtgac accacttctt ctttcagcac
     6241 attcgcaccc gcacgggttg cctcacgcag cacccggtta ttttccgcac cactcagaag
     6301 ctgcaaatcg cggctgatgt cctccagccc cgaaaaatcc agcagggttt cgatcatttt
     6361 tcccctccca gccgacagag aatttccaga cgcccgccgg tcgcatccgg cacgggcagc
     6421 ccgacaacgt tcaggatccg gtcacgccat ggaccactca gcacatgaag tcgtgacgct
     6481 gccgtgattt cccgaccgga ctgaccgcgc acccagatgc ggatttccgc ctgcgccatt
     6541 tccgcaccgg actgcatccg ctcccggctg ctcctgccac ggatatccgc atgaattttc
     6601 ccgcatgaca cccattcttc cgtcatttct ccggcagcat tacgggttaa caccgggttc
     6661 agaacactta tcatctgtgt cagacgacct gcagatattg ccattccccc tcctcataac
     6721 accgtcggac aacgcaaatc gtaaatcagc acggaaacag aaaacggcag ctccccctga
     6781 agcagttctt cccgctccgc aagatccgga ttccggtaca gcatcccggt cagtcgcatg
     6841 gcagccccct tcatccgggt taatgcctcg cccgggatca gttcaccgtc ctcacgaatc
     6901 accttatccc ggctgccctg aatgtaggcc agcagcacgg cggtagcctg acgaaccttg
     6961 tccatcagca tgtcatcatc cgcgtcatgg tcgacacgca gatgtgcctt gatctcttcc
     7021 agcgtcagta atgccgtcat tttccgcctc ctgcatcccg cccacgtttt gcagccaggg
     7081 tccagcctga tgaatgagct tctccgggtt tatcaccggt catactgttg cagtgccaca
     7141 gcgagccccc ccacgtcacc gtatcgccgg ggtggtaggt ttcaccggct ctgaacacac
     7201 cgcggtagag catcaccggc agggaaaatg ttttttccgt acactggcca ctgctctgcc
     7261 ggaccaccac agagaacaac cgttcacccg tcatgctgac gtcaatatcc gccaccccgt
     7321 caaccaggca ttcccatccc cgcatcccgt gcgttttttc atacgcccgc cagagtccgc
     7381 cctggtgtgt ggcatacgtg ccccggggaa aggatttttg atcgtcaatg gcagggagta
     7441 tttccagtgc agtggcatca cgcccgtcct gcggagccgg cagggcactc accgcatcca
     7501 gaacagcctt ctgcagaaca tcgggatcat agtcacgacc atcacgcgga acaggaatat
     7561 ggcttaccgc ctccttcacc atctgttcaa gcatcggacg ca
//
LOCUS       DAZKIV010000183         7540 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZKIV010000183 DAZKIV010000000
VERSION     DAZKIV010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.183"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..2605
                     /gene="pgaA"
                     /locus_tag="ACHOT6_004197"
     CDS             182..2605
                     /gene="pgaA"
                     /locus_tag="ACHOT6_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFP9949314.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2614..4632
                     /gene="pgaB"
                     /locus_tag="ACHOT6_004198"
     CDS             2614..4632
                     /gene="pgaB"
                     /locus_tag="ACHOT6_004198"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFP9949315.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4625..5950
                     /gene="pgaC"
                     /locus_tag="ACHOT6_004199"
     CDS             4625..5950
                     /gene="pgaC"
                     /locus_tag="ACHOT6_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFP9949316.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            5952..6365
                     /gene="pgaD"
                     /locus_tag="ACHOT6_004200"
     CDS             5952..6365
                     /gene="pgaD"
                     /locus_tag="ACHOT6_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFP9949317.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6415..7203)
                     /gene="phoH"
                     /locus_tag="ACHOT6_004201"
     CDS             complement(6415..7203)
                     /gene="phoH"
                     /locus_tag="ACHOT6_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HFP9949318.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
BASE COUNT         2035 a         1748 c         1778 g         1979 t
ORIGIN      
        1 ttcgggcgag aaaaggattt tatatggaca ctctgctcat catttcttct tctcatcatc
       61 aacaattcac gtctctcttc cgcgtttaat aacggattat gaggtgcaaa aatatctttc
      121 ttttcagtta cctgtaatta gatacagaga gagattttgg caatacatgg agtaatacag
      181 gatgtattca agtagcagaa aaaggtgccc gaaaaccaaa tgggctttga aacttcttac
      241 tgccgcattt ttagcagcga gtcccgcggc gaagagtgct gttaataacg cctatgatgc
      301 attgattatt gaagctcgca agggtaatac tcagccagct ttgtcatggt ttgcactaaa
      361 atcagcactc agcaataacc aaattgctga ctggttacag attgccttat gggccgggca
      421 agataaacag gttattaccg tttacaaccg ctaccgtcat cagcaattac cagcgcgtgg
      481 ttatgcagct gtcgccgtcg cttatcgtaa cctgcaacaa tggcaaaact cgcttacact
      541 gtggcaaaag gcgctctctc tggagccgca aaataaggat tatcaacggg gacaaatttt
      601 aaccctggca gatgctggtc actatgatac tgcgctggtt aaacttaagc agcttaactc
      661 tggagcaccg gacaaagcca atttactcgc agaagcctat atctataaac tggcggggcg
      721 tcatcaggat gaattacggg cgatgacaga gtcattacct gaaaatgcat ctacgcaaca
      781 atatcccaca gaatacgtgc aggcattacg taataatcaa cttgctgccg cgattgacga
      841 tgccaattta acgccagata ttcgcgctga tattcatgcc gaactggtca gactgtcgtt
      901 tatgcctacg cgcagtgaaa gtgaacgtta tgccattgcc gatcgcgccc tcgcccaata
      961 cgctgcatta gaaattctgt ggcacgataa cccagaccgt actgcccagt accagcgtat
     1021 tcaggttgat catcttggcg cgttattaac tcgcgatcgt tataaagacg ttatttctca
     1081 ctatcagcga ttaaaaaaga cggggcaaat tattccgccc tgggggcaat attgggttgc
     1141 atcggcttat ctcaaagatc atcagccgaa aaaagcacag tcaataatga ccgagctctt
     1201 ttatcacaag gagaccattg ccccggattt atccgatgaa gaacttgcgg atctctttta
     1261 cagccacctg gagagtgaaa attatccggg cgcgctaact gtcacccaac ataccattaa
     1321 tacttcgccg cctttccttc ggttaatggg cacgcctacg agcatcccga atgatacctg
     1381 gttacagggg cattcgtttc tctcaaccgt agcaaaatat agtaatgatc ttcctcaggc
     1441 tgaaatgaca gccagagagc ttgcttataa cgcaccagga aatcagggac tgcgcattga
     1501 ttacgcgagt gtgttacaag cccgcggttg gcctcgtgca gcagaaaatg aattaaaaaa
     1561 agcagaagtg atcgagccac gtaatattaa tctggaggtt gaacaagcct ggacagcatt
     1621 aacgttacaa gaatggcagc aggcagctgt cttaacgcac gatgttgtcg aacgtgaacc
     1681 gcaagatccc ggcgttgtac gattaaaacg tgcggttgat gtacataatc ttgcagagct
     1741 tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg gatagtggta aacatgatgt
     1801 cgacttaacc accatcgttt attcaccacc gctgaaggat aactggcgcg gttttgctgg
     1861 attcggttat gccgatggac aatttagcga aggaaaaggg attgttcgcg actggcttgc
     1921 gggtgttgag tggcggtcac gtaatatctg gctcgaggca gagtacgctg aacgcgtttt
     1981 caatcatgag cataaacccg gcgcgcgcct gtctggctgg tatgatttta atgataactg
     2041 gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt ccattacggg caatgaaaaa
     2101 tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg tatcaaaatg agcggcgtaa
     2161 gtacggtgtc tcctgggctt tcactgattt ttccgacagt aaccagcgtc atgaagtctc
     2221 acttgagggt caggaacgca tctggtcttc accatatttg attgtcgatt tcctacccag
     2281 tctgtattac gaacaaaata cagaacacga taccccatac tacaacccta taaaaacgtt
     2341 cgatattgtt ccggcatttg aggcaagcca tttgttatgg cgaagctatg aaaatagctg
     2401 ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa aaacattatg gcacggatgt
     2461 cgtcacccaa ctcggctacg ggcaacgcat tagctggaat gacgtgattg atgctggcgc
     2521 aacgctacgc tgggaaaaac gaccttatga cggtgacaga gaacacaact tatacgttga
     2581 attcgatatg acattcagat tttaaggata aatatgttac gtaatggaaa taaatatctc
     2641 ctgatgctgg tgagtataat tatgctcacc gcgtgcatta gccagtcaag aacatcattt
     2701 ataccgccac aggatcgcga atctttactc gccgagcaac cgtggccgca taatggtttt
     2761 gtagcgattt catggcataa cgttgaagac gaagctgccg accagcgttt tatgtcagtg
     2821 cggacatcag cactgcgtga acaatttgcc tggctgcgcg agaacggtta tcaaccggtc
     2881 agtattgctc aaattcgtga agcacatcga ggaggaaaac cgctaccgga aaaagctgta
     2941 gtgctgactt ttgatgacgg ctaccagagt ttttataccc gcgtcttccc aattcttcag
     3001 gccttccagt ggcctgctgt atgggccccc gtcggcagtt gggtcgatac gccagcggat
     3061 aaacaagtaa aatttggcga tgagttggtc gatcgagaat attttgccac gtggcaacaa
     3121 gtgcgagaag ttgcgcgttc ccggctcgtt gagctcgctt ctcatacatg gaattctcac
     3181 tacggtattc aggctaatgc caccggcagc ttattgcctg tatatgtaaa tcgtgcatat
     3241 tttactgacc acgcacggta tgaaaccgca gcagaatacc gggaaagaat tcgtctggat
     3301 gctgtaaaaa tgacggaata cctgcgtaca aaggttgagg taaatccaca cgtttttgtt
     3361 tggccttatg gcgaagcgaa tggcatagcg atagaggaat taaaaaaact cggttatgac
     3421 atgttcttca cccttgaatc aggtttggca aatgcgtcgc aattggattc cattccgcgg
     3481 gtattaatcg ccaataatcc ctcattaaaa gagtttgccc agcaaattat taccgtacag
     3541 gaaaaatcac cacaacggat aatgcatatc gatcttgatt acgtttatga cgaaaacctc
     3601 cagcaaatgg atcgcaatat tgatgtgcta attcagcggg tgaaagatat gcaaatatca
     3661 accgtgtatt tgcaggcatt tgctgatccc gatggtgatg ggctggtcaa agaggtctgg
     3721 tttccaaatc gtttgctacc aatgaaagca gatattttta gtcgggttgc ctggcaatta
     3781 cgtacccgct caggtgtaaa catctatgcg tggatgccag tattaagctg ggatttagat
     3841 cccacattaa cgcgagtaaa atacttacca acaggggaga aaaaagcaca aattcatcct
     3901 gaacaatatc accgtctctc tcctttcgat gacagagtca gagcacaagt tggcatgtta
     3961 tatgaagatc ttgccggaca tgctgctttt gatggcatat tgttccacga tgatgctttg
     4021 ctttcagatt atgaagatgc cagtgcaccg gctatcacgg cttatcagca agcaggcttt
     4081 agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat ttaaacagtg ggcccgcttt
     4141 aaaagtcgtg cgttaactga cttcacttta gaacttagtg cgcgcgtaaa agccattcgc
     4201 ggtccacata ttaaaactgc acgaaatatt tttgcacttc cggtaataca acctgaaagt
     4261 gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa gctatgactg gaccgctatt
     4321 atggctatgc cttatctgga aggtgtcgca gaaaaatcgg ctgaccaatg gttaatacaa
     4381 ttgaccaatc aaattaaaaa catccctcag gctaaagaca aatctatttt agaattacag
     4441 gcacaaaact ggcagaaaaa tggtcagcat caggctattt cttcgcaaca actcgctcac
     4501 tggatgagcc tattacaact gaatggagtg aaaaactatg gttattatcc cgacaatttt
     4561 ctgcataacc aacctgaaat agaccttatt cgtcctgagt tttcaacagc ctggtatccg
     4621 aaaaatgatt aatcgcatcg tatcgttttt tatattatgt ctggtgttat gcatacccct
     4681 atgcgtagcg tactttcact ctggtgaact gatgatgagg ttcgttttct tctggccgtt
     4741 ttttatgtcc attatgtgga ttgttggcgg cgtctatttc tgggtctatc gtgaacgcca
     4801 ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa gataatccgt ctatctccat
     4861 tatcattccc tgttttaatg aggagaaaaa cgttgaggaa accatacacg ccgctttagc
     4921 acagcgttat gagaacattg aagttattgc cgtaaatgac ggttcaacag ataaaacccg
     4981 tgccatcctg gatcgcatgg ctgcacaaat tccccatttg cgggtcattc atctggcgca
     5041 aaaccagggg aaagccattg cgcttaaaac cggagctgcc gcggcgaaaa gtgaatatct
     5101 ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg gcggcatata ttgtggaacc
     5161 gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat cctcgtattc gaacacgttc
     5221 taccctggtg ggtaaaattc aggttggcga gtattcctca attattggtt tgatcaagcg
     5281 aacccagcgt atctatggaa acgtatttac cgtttccggt gttattgccg catttcgtcg
     5341 cagcgccctg gcagaagtgg gttactggag tgacgatatg atcaccgaag atattgatat
     5401 tagctggaag ctgcagttga atcagtggac gattttttac gagccacggg cgctgtgctg
     5461 gatattaatg cctgaaacgt taaaagggct gtggaaacag cgcctgcgct gggctcaggg
     5521 cggtgcagaa gtattcctca aaaatatgac aaggttgtgg cgcaaagaaa actttcgaat
     5581 gtggccgctg ttttttgaat actgcctgac gacaatatgg gccttcacct gcctggtcgg
     5641 tttcattatt tacgcagtcc aacttgccgg tgtaccgtta aatattgaat tgacacatat
     5701 cgctgcgaca catactgccg gaatattatt gtgtacgtta tgtttactgc aatttattgt
     5761 cagcctgatg atcgagaatc gctatgagca taatctgact tcatcgcttt tctggattat
     5821 ttggttcccg gttattttct ggatgctgag cctggcaacg acattggtat catttacacg
     5881 agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta agtcccgatc gcgggattct
     5941 gagaggttaa tatgaacaat ttaattatta cgacccgaca atcaccagta cgtttactgg
     6001 ttgattatgt tgccacaacc atcttgtgga cattatttgc gttgttcata ttcttattcg
     6061 ccatggatct gctgacgggt tattactggc aaagcgaggc cagaagccga cttcagttct
     6121 attttttgct ggcagtggcg aatgccgtcg tgttaattgt ctgggcgctg tacaataagc
     6181 tgcgttttca aaaacagcag catcatgcag cctaccaata tacgccgcaa gaatatgcag
     6241 agagcttagc aatacctgat gagctctatc agcaactaca aaaaagccac aggatgagcg
     6301 tacacttcac cagccagggg caaataaaaa tggttgtttc agaaaaagcg ctagtccggg
     6361 cataaacacc caaaacaaag cccggttcgc ccgggctctg caccgataat acacttaact
     6421 gtatgcatgc agcgtacgtt ggcaaagtgc cgaacgtacg cagtcctctt taccgaaccg
     6481 gacgatccca accatttcat cttcttcgaa acgttccagc gcgtcactta atccggagca
     6541 cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg acgataaccg tcacgttctc
     6601 cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc acattctgcg cctcgtcaag
     6661 aatgacgact gcattttcaa aggtacgtcc acgcatatag gcgaacggcg caatttccac
     6721 cttccctatt tccggtcgca ggcagtactg cataaaggaa gcccctaagc gccggaccag
     6781 cacgtcgtag accgggcgaa aatagggagc aaacttttct gcgatatctc caggtaagaa
     6841 gccaagatct tcatcggctt gcagaactgg acgggtgacg ataatcctgt cgacatcctt
     6901 atgtatcagg gcttctgccg cttttgctgc gctgatccag gtttttccgc acccggcttc
     6961 gcccgtggcg aatatcagct gcttactctc aatagccttc agatagtgca attgcgcttc
     7021 atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg gccataccaa tggcttctac
     7081 gccgcccatc tgcacaagcg aggtgaccga ttcttcttca cgctgcttat ggctgcgcga
     7141 atcccgtctc agcacacgtt ttgcctcgcg acgagctttg atcactgctt tttgtcttcc
     7201 catggagagc accttgagtt gtttgtattc atcacacgcg ccgttggcag cgcgattatg
     7261 cgcacgaaca tcagagggtt ggcttccttg taagccatag tttgcttttg gataaaatgc
     7321 cgaaaaacgg ctacgcgcac cgtttacggc gtcggtaaca catgaaaaga aaggatgagg
     7381 ttgaaaatgc aaagtgacga gatgactacc ggaggagaaa actccgcgag tggtggcgcg
     7441 ttgattatct aaaacatgtc cagtacagga cgttaccatc cgcgatctcc atagtgactg
     7501 actatcactg ccgggaactt ccgctgctac ttaataagta
//
LOCUS       DAZKIV010000184         7482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZKIV010000184 DAZKIV010000000
VERSION     DAZKIV010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.184"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(39..863)
                     /locus_tag="ACHOT6_004202"
     CDS             complement(39..863)
                     /locus_tag="ACHOT6_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HFP9949319.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSTTAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(916..2634)
                     /gene="proS"
                     /locus_tag="ACHOT6_004203"
     CDS             complement(916..2634)
                     /gene="proS"
                     /locus_tag="ACHOT6_004203"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFP9949320.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFSLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMNDFAAGANIDGKHYFGINWDRDVATPEIADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2745..3452)
                     /gene="tsaA"
                     /locus_tag="ACHOT6_004204"
     CDS             complement(2745..3452)
                     /gene="tsaA"
                     /locus_tag="ACHOT6_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFP9949321.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MPVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEGTGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3449..3853)
                     /gene="rcsF"
                     /locus_tag="ACHOT6_004205"
     CDS             complement(3449..3853)
                     /gene="rcsF"
                     /locus_tag="ACHOT6_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFP9949322.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(3971..4786)
                     /gene="metQ"
                     /locus_tag="ACHOT6_004206"
     CDS             complement(3971..4786)
                     /gene="metQ"
                     /locus_tag="ACHOT6_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFP9949323.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4826..5479)
                     /locus_tag="ACHOT6_004207"
     CDS             complement(4826..5479)
                     /locus_tag="ACHOT6_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFP9949324.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5472..6503)
                     /gene="metN"
                     /locus_tag="ACHOT6_004208"
     CDS             complement(5472..6503)
                     /gene="metN"
                     /locus_tag="ACHOT6_004208"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFP9949325.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6691..7266
                     /gene="gmhB"
                     /locus_tag="ACHOT6_004209"
     CDS             6691..7266
                     /gene="gmhB"
                     /locus_tag="ACHOT6_004209"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFP9949326.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1835 a         1935 c         1909 g         1803 t
ORIGIN      
        1 acgccgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag cccaacaaag
       61 cgacccgcct tgatataaat cccaggcttc aggtgcccaa catactgtaa ctgcgtttct
      121 ggcttaaacg cagagacatc accaatacgc atatgcatca gatcggcagg gctaatccag
      181 cccgattgcg ccagtgtaag tggatgcccg gctttggcaa aggcatccgt gacaaattcc
      241 gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc tttcgccagg
      301 ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca catctggcga
      361 gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataaccgct atctttgatt
      421 ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg gactcgtaag
      481 acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg gacgccagcg
      541 cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact cacagaggaa
      601 gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa cagcaaatcg
      661 ccgggtttga gatcaggtgc cgttatttct tttattgatt gttcggtgaa agagctttga
      721 tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggt tgatgggtct
      781 ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag aagcaggcgg
      841 cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc ctgaaattca
      901 ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga tgtcaccagt
      961 cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt cgttgtcgag
     1021 gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat cagcaaacat
     1081 cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg cacgcagttc
     1141 gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt tcatcggcag
     1201 aatcgccacc tggaacggcg cgatcgcgtc aggccatacg atgccgcgtt cgtcgtagtt
     1261 ctgctcaatc gctgcagcta ctacacgcgt taccccgata ccgtagcaac ccatagtcag
     1321 gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg agtacttggt
     1381 acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg taccctggcc
     1441 atccgggctt ggatcgccag cgaccacgtt gcggatatca gcaatttccg gggtagcgac
     1501 atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac cagcagcgaa
     1561 atcattcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt ttaccggacc
     1621 cagtgaaccc ggaccggctt taaccacggc acgaatttct tcttcggtcg cgaaagtcag
     1681 cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt gatcaccgcg
     1741 caccagcagc gcaaccagcg ggaagctact gccttcaacc gctttaacca gcagagtctt
     1801 aaccgttttc tcaatcggca gactgaactg ttcaaccagt tccgcgatgg ttttcgcgtt
     1861 cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt tcggcgcgat
     1921 agcttctgcc agctcaatgt tcgctgcata gtcagaggtg tcggagaaga ccacatcgtc
     1981 ttcaccgctt tgcgccagca cctggaattc gtgagaggcg ctgccgccga tagaaccggt
     2041 gtcggcttgt acggcgcgga aatccagccc catgcggctg aagattttgc tgtaggccgc
     2101 atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag agtaagcatc
     2161 tttcataagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt cgtcgcggaa
     2221 cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa gctcgttacg
     2281 aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac gctcgccacg
     2341 gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct cttgccacaa
     2401 atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt tcatctcttc
     2461 acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc aggtatataa
     2521 cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc tgatcacctc
     2581 ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac gcatgttgtt
     2641 acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa aaagtgattt
     2701 agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg cggttccagc
     2761 gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc atgcagccag
     2821 acggcatagg ttttgcccgt tccctcacct ttacgatagg ccgggcgcgg gtcctgcgcc
     2881 agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt caaaagctgc
     2941 ttttcgacct ctgcggtaaa actcaccggc atttctgccg ctggcgcact ttgcgcatag
     3001 ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat atccaccacc
     3061 ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc tttatggcaa
     3121 acaacctctt tcagctctac cagcgacata ccaattggat taggacggaa agtagagcgt
     3181 gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac agtcggacgc
     3241 cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc acaaatggct gaacgcttcc
     3301 aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc gccgttggcg
     3361 ctttttacca gacctggctg gcgcggaacg gcgaattttt ctttataggg cgagcgaata
     3421 acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc gcagaaccga
     3481 tacatacagc ctgacgatag cagcctggcg taccgctggt gacttcgcag ctatgcagta
     3541 ataccgcatt ggccttcatt ttcgaggcgt tgatttgcat ccgcttacgt gcggttggaa
     3601 tgctcggcgg agagtcctga ttggaggcct ggcaagaatc gccactgact tcaccgagat
     3661 cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc ggcgtggcgc
     3721 gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt tgaacgggtt
     3781 cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact aaacagatcg
     3841 gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg aatcaggagc
     3901 ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa ttgtggaaac
     3961 agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt gctgcttcgt
     4021 aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg tctttgttat
     4081 cttcacgcgt tacgatcagg tttacgtacg gggagtcttt atcttcgaca aagataccgt
     4141 ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact gccagagcga
     4201 tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca attttcagat
     4261 tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca tctttcagtt
     4321 tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt ggatcgtttg
     4381 gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc ttggagtaac
     4441 cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca cgatctttca
     4501 gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg cctttgctca
     4561 atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc aggccatatt
     4621 tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca acaatcacgc
     4681 cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt gccagtgatc
     4741 caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct tattccttct
     4801 tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg atgcggtcgc
     4861 ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta ttcatcaccg
     4921 tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca ccggcaccga
     4981 ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt gccgcattca
     5041 ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt ggcgtggcac
     5101 ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg ttctcgacca
     5161 tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc tgcaaaccaa
     5221 tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag ataatgaacg
     5281 ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc gcgttagcaa
     5341 taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca atcacaaagc
     5401 caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga accagcagcc
     5461 acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc ctgcagccag
     5521 gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat gatgccgaat
     5581 ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac gttgaaacga
     5641 cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc cagacgcagc
     5701 atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc ttccgggata
     5761 tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg atgcgagaac
     5821 acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc cacgcaatca
     5881 caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa acccagacgg
     5941 cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc cagcgcgctg
     6001 gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc aatcgccaca
     6061 cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc gccaagacca
     6121 accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt gttgtccagc
     6181 tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt aaaatgctgg
     6241 aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag cgtggtcagt
     6301 tcctggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt tacacaacgt
     6361 ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg tccagctggc
     6421 acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg gaacactttg
     6481 gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta gtcgtggcat
     6541 ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca tccagacgtc
     6601 taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg cgatactagc
     6661 gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc aatttttctt
     6721 gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga caactttgaa
     6781 tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt tgcgctggtg
     6841 gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca gtttgaaacg
     6901 ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga tggtatctat
     6961 tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg cgactgccgt
     7021 aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga tatggccgct
     7081 tcttatatgg tgggcgataa attagaagat atgcaggcag cggctgcggc gaacgtggga
     7141 acaaaagtgc tggtgcgtac gggtaaacct attacgcctg aagcagaaaa cgcggcagat
     7201 tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca aaaaccggcg
     7261 caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt cttcctgagc
     7321 cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca cacaaacagc
     7381 cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag aggaaagcgt
     7441 aatatacgcc accccgcgac agtgagctga aagctgcgtc gc
//
LOCUS       DAZKIV010000185         7348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZKIV010000185 DAZKIV010000000
VERSION     DAZKIV010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.185"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            490..1479
                     /gene="araF"
                     /locus_tag="ACHOT6_004210"
     CDS             490..1479
                     /gene="araF"
                     /locus_tag="ACHOT6_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFP9949327.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTMGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1549..3063
                     /gene="araG"
                     /locus_tag="ACHOT6_004211"
     CDS             1549..3063
                     /gene="araG"
                     /locus_tag="ACHOT6_004211"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFP9949328.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDISKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTLG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3078..4064
                     /gene="araH"
                     /locus_tag="ACHOT6_004212"
     CDS             3078..4064
                     /gene="araH"
                     /locus_tag="ACHOT6_004212"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFP9949329.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4231..5031
                     /gene="otsB"
                     /locus_tag="ACHOT6_004213"
     CDS             4231..5031
                     /gene="otsB"
                     /locus_tag="ACHOT6_004213"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFP9949330.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELETKGMAFALHYRQAPQHEDALMTLAQRITQNWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5006..6430
                     /gene="otsA"
                     /locus_tag="ACHOT6_004214"
     CDS             5006..6430
                     /gene="otsA"
                     /locus_tag="ACHOT6_004214"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFP9949331.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6437..6865)
                     /gene="uspC"
                     /locus_tag="ACHOT6_004215"
     CDS             complement(6437..6865)
                     /gene="uspC"
                     /locus_tag="ACHOT6_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFP9949332.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1874 a         1681 c         1877 g         1916 t
ORIGIN      
        1 acgttttttc atttttgcga gatggctctc attatacgtg ttctgttaat taatgagcac
       61 agtgataatt aatggcgaaa tgaatgacgt gcattttcca catctttgag ttgcggttat
      121 tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt aaaaagaaga
      181 catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg gattaatctg
      241 ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt cccgctaaat
      301 ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg agaattaatt
      361 tctcgctaaa actatgtcaa cacggtcact tatcttttag ttaaaaggta atgctttgtt
      421 ttccgattaa tttaacgaat gtcattcgtt tttgccctac acaaaacgac actaaagctg
      481 gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc agccgttatg
      541 tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca accggaagag
      601 ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt agggtttgag
      661 gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga cagcctggct
      721 gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg ctctgccatc
      781 gtcgcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca gtttgttaac
      841 gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac taaaattggc
      901 gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga tgtcaaagaa
      961 agcgcggtga tggcgattac cgccaacgaa ctggataccg cccgccgccg tacaatgggt
     1021 tctatggacg cactgaaagc tgcaggattc ccggaaaaac aaatttatca ggtacctacc
     1081 aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt tcaacatccg
     1141 gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg cggcgtacgc
     1201 gcgacggaag gtcagggctt taaagctgcc gatatcatcg gcattggcat taacggtgtg
     1261 gatgcggtga gcgaactgtc taaagcacag gcaaccggct tctacggttc cctgctgcca
     1321 agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt agcaaaagac
     1381 gttgaaccgc caaaatttac cgaagttacc gacgtggtgc tgatcacgcg tgacaacttt
     1441 aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa aattcccctc
     1501 tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat gcaacagtct
     1561 accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa ggcgctgacg
     1621 gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga aaatggcgca
     1681 ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac gggttctgta
     1741 gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa cgcgggtgtg
     1801 gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga aaacatctat
     1861 ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa ttatgaggcg
     1921 ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct gaaatatctc
     1981 tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa cgctaaaatt
     2041 atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa tcttttccgc
     2101 gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca ccgtatggaa
     2161 gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta tgtcaaaacc
     2221 tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt cgggcgcgac
     2281 attggcgata tctacggctg gcaacctcgt agttatggcg aggagcgcct gcgtcttgat
     2341 gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag tggtgaaatt
     2401 gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg cttgtttggc
     2461 gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga tattagtaaa
     2521 ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa agcggaaggc
     2581 attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg taaacatgtg
     2641 ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca gcacattcgt
     2701 tcgctcaaca tcaaaacgct gggcgctgag caactgatca tgaatctctc aggcggaaat
     2761 cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat tttgctggat
     2821 gaacctacgc gcggcattga tgttggggct aagcatgaaa tttacaacgt gatttatgcg
     2881 ctggcggcgc agggtgtggc ggtgctgttt gcctccagtg acttacctga agtcctcggc
     2941 gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt gttacacgag
     3001 caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca ggctgttgcc
     3061 tgagtaagga gagtatgatg tcttctgttt ctacatcggg gtctggcgca cctaagtcgt
     3121 cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt gcggtgctct
     3181 ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg aaagggttgg
     3241 gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc cttgcttccg
     3301 gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc acggcggtgg
     3361 ttatcaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg cttggcattc
     3421 tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct ctgatcacga
     3481 cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac ggtaaagcgg
     3541 tcggtatcga agatgaaagc ttctttgccc ttggttacgc caactggttc ggtctgcctg
     3601 cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgctgctg aataaaacca
     3661 cctttggtcg taataccctg gcgattggcg ggaacgaaga ggctgcgcgt ctggcgggtg
     3721 taccggttgt tcgcaccaaa attattatct ttgttctctc tggcctggta tctgcgatag
     3781 ccggaattat tctggcttca cgtatgacta gtgggcagcc aatgacgtcg attggttatg
     3841 agcttattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt ggcatcggaa
     3901 aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac gccatgaacc
     3961 tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg cttaatcctg ctggcagcgg
     4021 tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt tttccacaac
     4081 aatttaacgt tttttcccac cacagccagc cgccacaacg gttggctgtt cttcattgca
     4141 aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt tctgcgcaaa
     4201 acaataagaa aagagaagga ggagagccgg gtgacagaac cgttaaccga aacccctgaa
     4261 ctatccgcga aatatgcctg gttttttgat cttgatggaa cgctggcgga aatcaaaccg
     4321 catcccgatc aggtcgtcgt gcctgacaat attctgcaag gactacagct actggcaacc
     4381 gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct tgacgcactg
     4441 gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg tgacatcaat
     4501 ggtaaaacac atatcgttca tctgccggat gcgattgcgc gtgatattag cgtgcaactg
     4561 catacggtca tcgcccagta tcccggtgcg gagctggaga cgaaagggat ggcttttgcg
     4621 ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc gcaacgtatt
     4681 actcagaact ggccacaaat ggcgttacag cagggaaagt gtgttgtcga gatcaaaccg
     4741 agaggtacca gtaaaggtga ggcgattgca gcttttatgc aggaagctcc ctttatcggg
     4801 cgaacgcccg tatttctggg cgatgattta accgatgaat ctggcttcgc agtcgttaac
     4861 cgactgggcg gaatgtcagt aaaaattggc acaggtgcaa ctcaggcatc atggcgactg
     4921 gcgggtgtgc cggatgtctg gagctggctt gaaatgataa ccaccgcatt acaacaaaaa
     4981 agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta accggattgc
     5041 accaccagac gagcacgccg ccagtgccgg tggccttgcc gttggcatac tgggggcact
     5101 gaaagccgca ggcggactgt ggtttggctg gagtggtgaa acagggaatg aggatcagcc
     5161 gctaaaaaag gtgaaaaaag gtaacattac gtgggcctct tttaacctca gcgaacagga
     5221 ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcccgctt ttcattatcg
     5281 gctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg taaatgcgtt
     5341 gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga tccacgatta
     5401 tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc gcattggttt
     5461 ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga catatgacac
     5521 cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa acgatcgtct
     5581 ggcgttcctg gattgtcttt ctaacctgac ccgcgtcacg acacgtagcg caaaaagcca
     5641 tacagcctgg ggcaaagcat ttcgaacaga agtctacccg atcggcattg aaccgaaaga
     5701 aatagccaaa caggctgccg ggccactgcc gccaaaactg gcgcaactta aagcggaact
     5761 gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag gtttgccaga
     5821 gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg gtaaaattcg
     5881 ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg atattcgtca
     5941 tcagctcgaa aatgaagctg gacgaattaa tggtaaatac gggcaattag gctggacgcc
     6001 gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat tccgctactc
     6061 tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtagcaa aagagtatgt
     6121 tgctgctcag gacccagcca acccgggcgt tcttgttctt tcgcaatttg cgggagcggc
     6181 aaacgagtta acgtcggcgt taattgttaa cccctacgat cgtgacgaag ttgcagctgc
     6241 gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg cagaaatgct
     6301 ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta gcgacctaaa
     6361 gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta cctttccaaa
     6421 gcttgcgtag gagcggttaa tctcccgtaa gtggaactaa cagcacatcg acctcacttg
     6481 aggcaatcac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga ttgtgattac
     6541 cgcaaatcac caaatcgaaa tgatgtttgt gacatacttc cagaatatgt tcgcttaact
     6601 caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt aacttatcaa
     6661 gaaagctttg cgtttcttct tgcatcacgc tgcgtaaatc ttcaagcatc ggcgcggcta
     6721 attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt ccctttactg
     6781 gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta accgcaacag
     6841 cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt caggtgacat
     6901 taaagcttta ctcctaatgg agaaacgacg caatcccaac tcggtcaaac aggtaaatat
     6961 tagtctgatt attagtaaag acaattaatc aatcactccc ggtgatcata taatttcaaa
     7021 tgtaattata agaaaatagt gtactgattc tccgcattaa acaagtggtt aatatatgta
     7081 aaacatttca ttaacattaa gttgattgtt gcctttcttt gtatttaatt agtttgttgt
     7141 gcggtaagtg tctgtttaaa aatagcttat ctggtattgc atgaaagtga ttatttatag
     7201 cagatgatta tttacggtga gttattttaa ctgtgcgcaa catcccattt cgattattcc
     7261 tgtttcattt ttgcttgcta gcgtagcgaa aaacttttta acagattgaa atacacccaa
     7321 aacaaaagta tgacttatac atttatgt
//
LOCUS       DAZKIV010000186         7347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZKIV010000186 DAZKIV010000000
VERSION     DAZKIV010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.186"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(390..1526)
                     /locus_tag="ACHOT6_004216"
     CDS             complement(390..1526)
                     /locus_tag="ACHOT6_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFP9949333.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(1529..1891)
                     /locus_tag="ACHOT6_004217"
     CDS             complement(1529..1891)
                     /locus_tag="ACHOT6_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFP9949334.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2428..4341
                     /gene="mtlA"
                     /locus_tag="ACHOT6_004218"
     CDS             2428..4341
                     /gene="mtlA"
                     /locus_tag="ACHOT6_004218"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFP9949335.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLNSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4571..5719
                     /gene="mtlD"
                     /locus_tag="ACHOT6_004219"
     CDS             4571..5719
                     /gene="mtlD"
                     /locus_tag="ACHOT6_004219"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFP9949336.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKTWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYGLPHKNLIEGIAAAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5719..6306
                     /gene="mtlR"
                     /locus_tag="ACHOT6_004220"
     CDS             5719..6306
                     /gene="mtlR"
                     /locus_tag="ACHOT6_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFP9949337.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(6318..6527)
                     /gene="yibT"
                     /locus_tag="ACHOT6_004221"
     CDS             complement(6318..6527)
                     /gene="yibT"
                     /locus_tag="ACHOT6_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFP9949338.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6812..7174
                     /locus_tag="ACHOT6_004222"
     CDS             6812..7174
                     /locus_tag="ACHOT6_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFP9949339.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1879 a         1841 c         1849 g         1778 t
ORIGIN      
        1 gaatacgata attaaaagtg aagaagaaaa tagttgtata catatattcc atgaccctac
       61 attgtgatct atagattaaa tattttttaa atgtgactgt catccttcag ataataaagc
      121 catcatcgca atagatggaa aagcactcct gctctttttt gattagagcc gtcgcaggag
      181 gaacaactca tatcatcatt gcgatctcga cgatgtatag tctcatcctg cggcagaaca
      241 agacggataa aaaatccaat gagagcacac tcatccagaa cttattaaca taatgaatat
      301 taaagaaaaa tcatcgcagc taatgaagta atatgcaaaa ataattagtc cttacaagga
      361 gcaaagttcc ataatctccc ccctccccct caatgatcca aataaagata atgcatccag
      421 ctggtcattc ttagcagcac tttccgcatc accgaaacat ggctgaaatg gtcggagtaa
      481 accgccacct gggcgtagat gccgtcgggt aaggcatcga tatcatcgtt aggatccagt
      541 tcaatggttc ccagcacacc gtccgtgccg ggcacgaccg ttaatgattg caataccccc
      601 tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac tagtcagttt gccgtgaaac
      661 acctgcccag gtagcgcgtt aaacaccact tccgcatcat cgccgggttt cagacgtaac
      721 agcgagtttt gccgaaattg ggcgacaatt tgccgttttt gctcggggat gaagaccatc
      781 accggacgca gcggcaaggc agctgcgtat gtacctgggc ggatcagtac ctgagtaacg
      841 tagccattgc tcggcgcgcg aatgacagtc tgctcaaggt tatattttgc ttcagtaagt
      901 tgcgctctta agctcacaat ctgcgattgc tcgccgttaa ccatactgtc gagctggctc
      961 tggatctgcg cctgctccgc caccgagcct ttcaccagcg catcctgcgc gaggaaattt
     1021 tgccgcgcat cgtcgatgtc acgttccgag aacggattca ccgccgcctg gctgcctttc
     1081 aagtaacgtt gataattttt aaacagacgg tcgcgctccg ctgaaacctg ggtggtgttg
     1141 gcctgcgctt cagtgagctg cgcacgcagc gtctttatat tatgcgtcgc cgtcatcagg
     1201 tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg ggtcgagctt aaaaagcacc
     1261 tcgccctttt gaataagctg attattcttg tcagtgactt cagtaacaat tcccgtcacc
     1321 tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg taaaagtgta agggtggttg
     1381 tagttcatca acaaaatcaa accactcacc agaaacacgc ctcccagcgc cgccgtcgcc
     1441 agcgtccact gatttaccgg aatgcggaag attttaaaga ccgcccacgc cagcgccacg
     1501 taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa tagtcagtct
     1561 ttttctcggc ggcgagttga tgctccagcc gggaaattcg gtcagaaagt gaggcgattt
     1621 ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg cataccccag ccacgctccg
     1681 gttgatagag cgtcgcccag atccacagaa acggccagat aacatgcaga gtaaacaggc
     1741 tcacccagcc cgccgtatga atagcgtcgg catgggggtg attgcgcttt ttggcaatca
     1801 gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag acgaagatca
     1861 gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt ttatcagtgt
     1921 tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata atccgggttc
     1981 gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc ttagttacat
     2041 aacgattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac gccgtaagaa
     2101 aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa atgtgacact
     2161 actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt ttacacaagc
     2221 gttttgtgat gaacgtcacg tcaattacct ctctaccccc tatatttatg tgattgatat
     2281 cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc gcagtatcta
     2341 caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc agcctgcgcg
     2401 acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa agtgcaaagc
     2461 tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat cgcgtggggt
     2521 atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct ggcgaagctg
     2581 gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg tggtaagctg
     2641 gtaggcggcg aacgtggcgg cgtagtcggt gccatcacta ccatgggcgt tatcgtcggc
     2701 gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg ctggtgcatt
     2761 aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat gctggtgaat
     2821 aacttctccg caggcatcat cgggatgatc ctcgccattc tggcatttct cggcattggc
     2881 ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat ggttgtccat
     2941 gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt cctcaacaac
     3001 gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga actgggtaaa
     3061 tccatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct gctggcgtac
     3121 atgttctttg gtcgtggtag tgctaaacag tctgcgggcg gtgcggcaat catccacttc
     3181 ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg tctgatcctg
     3241 gccgttatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg cggtggtctg
     3301 gtttctccag catctccggg gtctatcctt gcggtactgg cgatgacgcc aaaaggtgct
     3361 tacttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt tgtctctgct
     3421 attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc aactcgtcgt
     3481 atgcaggaca tgaaagctga gtctaaaggc gcatctccgc tgtctgctgg cgatgtgact
     3541 aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat gggttccagt
     3601 gcgatgggcg caggcgttct gcgtaagaaa attcaggatg caggtctgtc gcagatttct
     3661 gttactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat cactcaccgt
     3721 gacctgaccg aacgcgctat gcgccaggtt ccgcaggcac agcatatttc gctgaccaac
     3781 ttcctcaaca gcggcctgta caccagcctg accgaacgtc tggttgctgc ccaacgccac
     3841 acggcaaacg aagagaaagt aaaagacagc ctgaaagaca gctttgacga ttccagtgct
     3901 aacctgttca agctaggcgc ggagaacatc ttcctcggtc gcaaagcggc aaccaaagaa
     3961 gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga gccggaatac
     4021 gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga gtctatcgcg
     4081 gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt cgtgttctgc
     4141 cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg tctggtgatt
     4201 ggtattgctg cccgtaacaa cgagcacatt caggttatca ccagcctgac caatgcactg
     4261 gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga agtgctggaa
     4321 ctgctggcag gtcgtaagta atccaatccc accctctcca catggagagg gtggggctaa
     4381 ttgcctgatg cgctacgctt atcaggccta caggatgcat cacaatttgt tgaatttgca
     4441 cgttcttgta ggccggataa ggcgtttacg ccgcatccgg cgctgcccct ctccacatgg
     4501 agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa cattgatgaa
     4561 ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg gctttatcgg
     4621 taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc aggtggtact
     4681 tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa ccgagcaggt
     4741 tgataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg ttgatctgat
     4801 tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac gtattgctcc
     4861 ggctatcgcc aaagggctgg tgaaacgtaa agaacaaggt aatgaatccc cgctgaacat
     4921 catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg tgatgaacgc
     4981 cctgccagaa gacgccaaaa cgtgggtaga agaacacgtt ggctttgtcg attccgccgt
     5041 tgaccgcatc gtgccgcctt cggcttcggc aactaacgat cctctggaag tgacggtaga
     5101 aaccttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc cgaacatccc
     5161 aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct tcaccctgaa
     5221 cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga ccattcgtga
     5281 cgcgattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag aaagtggtgc
     5341 agtattgatc aagcgctacg gctttgacgc tgacaagcat gcggcgtaca tccagaaaat
     5401 tctcggccgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag gccgtcagcc
     5461 actgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ctgctcggta cgctggaata
     5521 tggtctgcca cataaaaacc tgattgaagg tattgccgct gcaatgcact tccgcagtga
     5581 agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc cgcaggcggc
     5641 gctggcacag atttccggtc ttgatgccaa cagcgaggtt gtatccgagg cggtaaccgc
     5701 ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc gaataacaga
     5761 ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg tgtgcttgag
     5821 cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga gctcctgacc
     5881 gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc ggtgaagtat
     5941 gctgtagaac cgttactcga cggcgatggt ccgctgggcg atctttctgt gcgtttaaaa
     6001 ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga actgctgatg
     6061 gcattgcgtg aagagctaaa tcacgacggc aacgagtacg cctttaccga cgacgaaatc
     6121 cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc acagtttgaa
     6181 ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc tgtgcgatca
     6241 acaatggtcc tttcactgac tgagctgatt tccaaaatca gcttaaaaaa agcctttcaa
     6301 aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag ccgacgataa
     6361 tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga cggaatcgcg
     6421 ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc catgaaatct
     6481 acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat gatatctccg
     6541 tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc ggtaaagatg
     6601 acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat tatctttttt
     6661 ctatagattt cttaattaat caacgaattg cgtagttttt gagcatgtta atgattacgt
     6721 tacagaacta ttacaatgtc atcagttgtg aacaaagcac ctggtcgcgc atactagggg
     6781 ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa acgaaatcaa
     6841 acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt cgctggttga
     6901 agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag cagaaattgc
     6961 tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc tgatgaagat
     7021 gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc tgaagaaaag
     7081 tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa tgggcctgca
     7141 ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg gttcgatcat
     7201 atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg tctactcgcc
     7261 taattaccaa acaattcact ccctttactt cgatttaccg ctggttgata ttctgaaaat
     7321 gcatttttac cgaaatagcc atctata
//
LOCUS       DAZKIV010000187         7342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZKIV010000187 DAZKIV010000000
VERSION     DAZKIV010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.187"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHOT6_004223"
     CDS             complement(<1..386)
                     /gene="gpU"
                     /locus_tag="ACHOT6_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9949340.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKIT"
     gene            complement(399..1022)
                     /locus_tag="ACHOT6_004224"
     CDS             complement(399..1022)
                     /locus_tag="ACHOT6_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949341.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRAAATTLNRVAESIIAKTASSV
                     ARGLAVPRRLIRERIRLQRASADRVYAKVIINTGNLPAIKLGTASVRLSRRKRRKKGE
                     RSVTKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKIPTAGPLAETFERERDRMYREKLPVQMMKAMTHQLRLVLKRK"
     gene            complement(1025..1306)
                     /locus_tag="ACHOT6_004225"
     CDS             complement(1025..1306)
                     /locus_tag="ACHOT6_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFP9949342.1"
                     /translation="MSDLFTRMCCRMDVATVRMMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(1299..1625)
                     /locus_tag="ACHOT6_004226"
     CDS             complement(1299..1625)
                     /locus_tag="ACHOT6_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFP9949343.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(1713..3737)
                     /locus_tag="ACHOT6_004227"
     CDS             complement(1713..3737)
                     /locus_tag="ACHOT6_004227"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9949344.1"
                     /translation="MPELNDSISATPKASMKNKSWFRMQAGVPGDADIYIYDEIGFWG
                     VTAKQFVSELNALGDITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASM
                     ASVIAMAGDTVIMPENAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRDTTVTIPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMF
                     GGRYQTLQAQCVADPDCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIR
                     QAMLARAGFENVEKDNAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDF
                     GNILLDVSNKGLIQGWEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEY
                     KYITTSDRKETIALATYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKV
                     LTDNPKLSDGKALFHADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPTFMLVP
                     VALETVANQTIKSASVKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTD
                     TIEVAWLDGVDTPYIDQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(3682..5184)
                     /locus_tag="ACHOT6_004228"
     CDS             complement(3682..5184)
                     /locus_tag="ACHOT6_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9949345.1"
                     /translation="MAIIDDVIGVFSPRWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGIKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTERQYQRDTESQHEE"
     gene            complement(5184..5396)
                     /locus_tag="ACHOT6_004229"
     CDS             complement(5184..5396)
                     /locus_tag="ACHOT6_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949346.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(5393..6517)
                     /locus_tag="ACHOT6_004230"
                     /pseudo
     CDS             complement(5393..6517)
                     /locus_tag="ACHOT6_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
     gene            complement(6889..7170)
                     /locus_tag="ACHOT6_004231"
     CDS             complement(6889..7170)
                     /locus_tag="ACHOT6_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949347.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQATQPDSMTADNYVRVVVEVMR
                     GDGIQLNDVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWKTPERLRR"
BASE COUNT         1628 a         2280 c         1705 g         1729 t
ORIGIN      
        1 gtaattttcc acaggagatc tgccgatccc cacatcataa actcatcatc ccggcggtag
       61 tcatacccct gaagattcat cttcagcagt aatgcactga gcccgggaac tgcctccagc
      121 gcaggaagaa ttttttcttc catccacata tccagtgccg agtccggttc ttttgccctg
      181 agaaaaacct caatatgcag tgtcgcctcc caggttccct catcaacgaa ctcgtcagca
      241 gcagacgcat ctgtcaggta aacagcaaca gcaggcagtt cctgttcatc aataaaaacc
      301 gggcgaccgt caaaccagct caccctctca gaaatatttt ctttcagggc agacagaact
      361 gccgcccgta tttcacggtg tttcatacac cctcccagtc attttctttt cagcaccagg
      421 cgtaactgat gcgtcatggc tttcatcatc tgcaccggta atttttcccg gtacatccgg
      481 tcccgttcac gttcaaaggt ttctgccagc ggtccggcag tcggaatctt caccacttcg
      541 atcggcagac ggtggcgttt cggcctccct ttgctgtcag cgccggtgga cgatgatgcc
      601 cacggcatac gctgcatcac atgccagcgt ccgttagcca gccgggtgat aaaggcatcc
      661 gggatccgtc ttttccccac aatcagcaca ctgccaccgc ctttcgtgac cgaacgctcg
      721 cctttctttc gtcgctttct gcgggaaagc cgcacgctgg ccgtccccag ttttatggcg
      781 ggcagattac ctgtgttgat gatgaccttc gcataaaccc tgtctgcgct ggcccgttgc
      841 aggcggatac gctcacggat gagacggcgc ggaaccgcca gccccctggc aaccgaagag
      901 gccgttttcg cgatgatgga ctccgccaca cggttaagtg tcgtggcggc agcccgggga
      961 acggcacggc ggtcaattgc atccagattt ttcatggcct gcgccagacc ttttattgcc
     1021 atactcattc ctgttcgaca aaaatccggg gtttaccgtt gtacgtgtca taacgggtca
     1081 ccgtcagtgt gcgccccgca aacacaacaa catcatgacg ggccggacgg taccgggctg
     1141 aaaacaccac cagtgacaac tggctgcccg aaagcgcccc catctccgcg gactcttcct
     1201 ccggcatcac gtcgtacacg acgccgttaa tctccgcctg tttgcccatc atccgaacgg
     1261 tcgccacgtc catccggcaa cacattcgcg taaacagatc agacattgat ttttaccgcc
     1321 acagtggcgc tgtttgcagg agcattttcc caggctaccc ccgcggccac cgcaccgtcg
     1381 gcagccaact gcacaacccc gtccttcaga taaactaccg cgccggactg aatgtcgtca
     1441 gcagactgtt tgggcagcag gaacacgcct tcggcaaaac cgtcaccggc ctcaccggca
     1501 ggaatatcgg taatggccac ggccaccata ctgccgacca ccaccgcagc accgctcagg
     1561 atggtctgat ctccggcatt caccagttca atggtggtac cgtcctgtac aaaatttttc
     1621 gccataatgc tgtttctccg gacagcccct gtggggctgt ttttcaggca taaaaaaagc
     1681 cctttcgggc agtgattgtg ataacgcggt tatcaggcca ccgacgaacg caccagcccg
     1741 cgccagtcaa gtggtgccac tccggcatca atacggattt ttgtggcaat gccgtcagtg
     1801 gtgaaacctt cctgctgatc aatgtatggc gtgtccacac catccagcca ggccacttca
     1861 atggtgtcag tgccctgtgc cgccgccaga taccaggttt tcgggtctgc cgcatcaaga
     1921 cgcgcttctg caatcacctc agcaaagttc tggatagggt taatgacacc ggcgtttgca
     1981 tccgcccctt tcacactggc cgatttgatg gtctggttcg ccaccgtctc cagtgccacc
     2041 ggtaccagca taaaggtcgg acggatattc agggcacgat cgccttcttt ctgcaggcgc
     2101 atcatctgac gggccgcatc cagtccggaa acggagatcc ccccggtggc aatatttttg
     2161 tgatcggcat ggaacagcgc cttaccgtcg gacagtttcg ggttatccgt cagcaccttg
     2221 tagaccaggt caccaatcgt tgccttcgcc gcacgcccca tcttcatcgg cacgtccacc
     2281 agcatattca gatcatcatt gataatggcc tggcgggtga tggagaaaat ctccccgtaa
     2341 gtggccagtg caatggtctc cttgcgatct gaggtggtga tgtatttata ctccgccccc
     2401 tcacgaacct ggcgcagaga accaaaaccg cccatcccca cgcgatacgc tgttttgaag
     2461 tctgacaggc gtcccttacg ggtccacttc tggaaggttt cttctgattc ctcccagccc
     2521 tggatcagcc ccttgttcga cacatccagc agaatattgc caaaatcaga ggtgctgtgc
     2581 gtcagcgcca gcccgaccat ctgcatgggg ttataactgg ccaccccaat accgcgctcc
     2641 gtcagtgaca tgcgagccca ttcacgcagg gtcatcccgt tataggcgtt atccttctcg
     2701 acattttcaa atccggcacg ggccagcatc gcctggcgga tcccgtctcc cacaaaattg
     2761 ccgtttccgg cataaatatg ggccggtgta tttttgttgg tcggcgagga ctccttgccc
     2821 atttcattca gcagtcgttc acgggccatt tccagcgaac agtcaggatc agccacgcac
     2881 tgtgcctgaa gcgtctgata gcgaccgccg aacatggcaa acagatcgtt aatgcctgac
     2941 atgcgggctt tctgttctgc cataacgcgg gcgcgaatgg tcgcctcatc agacactgcc
     3001 ggtaccggtg atggttctgt taccgccggt gcagggattg tcactgtggt atcacgcggg
     3061 gcactgttgc gtggcggagt aatcatgttt cggatggatt ccggcatctt tttaaattcc
     3121 tctgtacgtt ttgactgaat acatgccatt gcctcaacag cgggtgtcac ctggtcagca
     3181 aatccgtgtg ccagacattc ggcaccggac atccaggttt catccgccag catggcggca
     3241 atttcatcgg tggtttttcc ggttttctgc gcataggctg gcaacagtac cgattcgact
     3301 ttatccagca aatcggcata actgcgcata tcctcagcat ccccgccact gaatccccat
     3361 ggcttatgga tcatcatgaa ggcattttcc ggcataatga ccgtatcacc ggccatcgca
     3421 atcacagatg ccatcgaggc ggcaacgcca tccacataca cggtaatggt cgccccctga
     3481 tttttcaggg cattaaaaat ggcgatgcct tcaaagacat cgccacccgg tgaattgata
     3541 tggagattaa tgtgggtgat atcacccaga gcattcagtt cgctgacaaa ctgcttcgcg
     3601 gtaactcccc agaaaccaat ctcgtcataa atataaatat ccgcgtcacc cggcacccca
     3661 gcctgcatcc tgaaccagga tttattcttc atgctggctt tcggtgtcgc gctgatactg
     3721 tcgttcagtt ccggcactgt tgcctccttt gtcgttgacg ggatccgtat caaagaccag
     3781 ccccagtctg ctgttttcat caatttcagc cttgcggcga cgtttgacct catccggatt
     3841 gcgcccgccg gcacgcaccc agtcagattc tgtcgctgca ccaccccgga tctgaattct
     3901 ccaggcttca gcttccttaa ccgggtcgat ccacggcatc accggaccgg aatacgtcgc
     3961 gttatatagc gttttcatct ccacatccgc cggaattttc agcagacctg ccgcaaccac
     4021 catattcagc catgtccggt acaccgggcg ggttaccgcg ccaataaaac agtcctgcag
     4081 gatcaggtaa ccatccgtgg actcgaccag ctcctgccgc tgggcgctgt aggtgccgtt
     4141 atagttacgc gccgcactgg aaaaactcag acgactgcct gctgccactg cacgcaactg
     4201 gccgttgcgg aaagtttcaa ggttgggatt gggacggtca gatttgacca tgccgatatc
     4261 ctcgcccttg cgcaaatcgt cataaataat acccggggtg atatggactt cccgctcggt
     4321 ttctttgatc cccggatctt catagtcctg tccgtcacct ttacggatat acagtcccag
     4381 cgccgcagca atacgcgccg ctgtcagttc cgcatcctca tactccttaa gggcactgat
     4441 ccgcatcagc acccccgata acatggatga gcctcgcgtc tggtgcagac gacgagtgaa
     4501 cttcaggtgg atcatttttc cggcagcgat ttctttcgta tcactctgcc ggccgctgac
     4561 cggataattt ttataaacca gatatttttt cggtcttccc cactcatcaa gaaaaacccc
     4621 ctgattcagt ccggcggatt catcagtgcg catgggaaca aaatccggct ccatcgcctc
     4681 aagccagaat ggcactcccg ccgtccgttc cagaccgttt cccgcaccac tgaccatctg
     4741 cgcaaacact tcaccatccc gcagccaggt ccgcagcagt aaacgttcaa gcacaggacg
     4801 ggtatactgc cctgtcacat ccggactcac ggaccattca gcccacaaac ggcggatatc
     4861 cgcagccagc tcagccgcca tttccccgtt ttttcgtaat ggctgaggct ccacaataat
     4921 tcccctggca ccaatcaccc gctcttccag cttgtcaaac acaccaatca ccaggtcatg
     4981 attgatatcc agaaaacggg cctgctcccg cagggaaacc gcaccgtatt tactgagctg
     5041 atcagcagag cgattttccc gccgggcttt atgtgtccgg gtcggtttca ccgcctcata
     5101 ggccatgatt aacgcccttg aacgcagtct ggctgctttc cacctagggg aaaacacgcc
     5161 gatcacatca tcaataattg ccattaaaac ctcgccagtt taaatcccgg ttttccccgc
     5221 ctgcggctca ccatcgcggc aagcctgcgt tcccactcct gacgtccggc gcggatctga
     5281 gaaaggcttt ccagcgtcag ttgctgcccg ttgaaggtga cagactttcc ctccagtacg
     5341 gccatttccg cttcacggta ccgctgtatc atttctctgg cttcttctgt gctcacaacc
     5401 agcctcctga tgttatccat ggattatctt ccgcacgctc cgtccgcagt tttttcttcc
     5461 ggcgacggcg tttttctgcc ccggccgtca gttccgggga taccgtttca ccagaacgct
     5521 cctgcgggaa gacgagccac gtttcccgct gtgcccagtc cggtgcggag ggccagcgga
     5581 tcttttcgta accatgcaga acggcaagcg catccgcata aaccagcagg tcaaacgctt
     5641 cgttagcgcc cctgcccggt tttcgccatt ttccgtcact gccgcgctct tcataggtca
     5701 gctcatcgta aaaccaccgc cccagccagt cgggaaagtg gatatagttc ggccctggtg
     5761 tgtcacgcca cagggcatta tttacacgat ccttaaacgc atccgtctga accagccaca
     5821 gcgcgacatc gccactggct ctggcacggc gggcacttct gccggtatta tccgggaagg
     5881 ttcggttaat cagcctgtca cggcgaagtc catccccctt gaacagaaac accctgttgc
     5941 ccagtccgtc actccggcaa cgacgccaga aacgataggc gttatctgtc accccggctt
     6001 cccctcccgt atccaccgcc atggccatca gacgcatgcg cacatccgga tcagaagcca
     6061 gcggccatgt tttatggaac acatccgtca gcaacaaatc ccagtcctcc ggatatgccg
     6121 ccggatcaac cggcagactt tcaccgttgg gactgcagcg cagtgaatgc cggatgttgt
     6181 agcgatcaac aatccagcgt tccccctgct ctccgtatcc ggtgatctgc acaacaaaac
     6241 ggcgattttt accgccctgt acgtcaaccg ttgcctcaat aaaacgcaca ccatccggca
     6301 cagatcgccg gggaaacggc tcggcacgct gttcaagcag ttcactttta cgctgttccg
     6361 tggctgaacg gggcagatag ggtcgtccga tatcggtgtt caccaccgct ttcagggtct
     6421 cttcactgcc ggttcgctca tactcttctt ctgccgccag cagtttaaaa atcagttgtt
     6481 cccaggtctg aaacgccgca gctggcccct ccatccaaga agaagtaaat aaaaggtaac
     6541 taaatgatta taaggtacta ttgagttatg ccccctacaa catagtccca gatttagtac
     6601 ctgcttaagc gataaaaaag caaaaaagga agcaatatta attattgccg ctatgcatga
     6661 tcacaatgac aaatcagagg cggaattccg ctctcggaac tcacatatga gaaacgatat
     6721 ttcttccacc gatacccttt atttgggtat cgcaagtaag ccccaaaatc tgggtgttgc
     6781 tcttactacc caaactacgg gtagtttccg taatttcatg atcgagttgc agatctgcaa
     6841 ctcacccacc agccaacgca attttgcgtt atcgggaata tcaacaagtt acctccgcag
     6901 ccgttccggc gtcttccact ggtaagtatt tttcgcgctc tccctccgtt gttgagaacg
     6961 gcgacggtat gccagcaact caaggactct ggttcgtata ttgcgcatat ccacatcatt
     7021 aagctggata ccatcaccgc gcatcacctc caccactaca cgcacataat tatctgcggt
     7081 catgctgtcc ggctgcgtgg cctgttcgtc aacctgctgg ctgattccgg cgacgcggcg
     7141 gattaatccc agtatttcgg cttctgtcat tgtgccccca tcgcgctggt gaagaaaaca
     7201 agctcagatt ttttgtaaag aatctgtcac gctaaaagat gtcgaacaaa aattaaccac
     7261 aaccatcatc ttttttgcat caaccacatt aaaaacaaca ggttacatac atgatgatga
     7321 tgacgataaa atcacaaaaa tg
//
LOCUS       DAZKIV010000188         7246 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZKIV010000188 DAZKIV010000000
VERSION     DAZKIV010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.188"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..433)
                     /gene="folK"
                     /locus_tag="ACHOT6_004232"
     CDS             complement(<1..433)
                     /gene="folK"
                     /locus_tag="ACHOT6_004232"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFP9949348.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETL"
     gene            complement(430..1827)
                     /gene="pcnB"
                     /locus_tag="ACHOT6_004233"
     CDS             complement(430..1827)
                     /gene="pcnB"
                     /locus_tag="ACHOT6_004233"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFP9949349.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1887..2783)
                     /gene="gluQRS"
                     /locus_tag="ACHOT6_004234"
     CDS             complement(1887..2783)
                     /gene="gluQRS"
                     /locus_tag="ACHOT6_004234"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFP9949350.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARHGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRELHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVSEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWTLTAVPESAIVNSTFSNASC"
     gene            complement(2850..3305)
                     /gene="dksA"
                     /locus_tag="ACHOT6_004235"
     CDS             complement(2850..3305)
                     /gene="dksA"
                     /locus_tag="ACHOT6_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFP9949351.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3483..4187)
                     /gene="sfsA"
                     /locus_tag="ACHOT6_004236"
     CDS             complement(3483..4187)
                     /gene="sfsA"
                     /locus_tag="ACHOT6_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFP9949352.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSISDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4202..4732)
                     /gene="thpR"
                     /locus_tag="ACHOT6_004237"
     CDS             complement(4202..4732)
                     /gene="thpR"
                     /locus_tag="ACHOT6_004237"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFP9949353.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4806..7235
                     /gene="hrpB"
                     /locus_tag="ACHOT6_004238"
     CDS             4806..7235
                     /gene="hrpB"
                     /locus_tag="ACHOT6_004238"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFP9949354.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSAILD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
BASE COUNT         1643 a         1899 c         2034 g         1670 t
ORIGIN      
        1 gcaacgtctc cccatcagga aacgccagct ccggtgcaat ctcaaacagc ggccacagca
       61 taaatccacg attcttcata tcgtagtgcg gaacggtcag gcgttcggta tttattactt
      121 cattaccaaa cagcatgatg tcgagatcca gcgtgcgcgg tccccagcgt tccgctttac
      181 ggacgcgacc ttgttgtaac tcaatacgct gagtgtgatt gagtagctct tcaggggcaa
      241 gagaggtttc cagcgctacg gctgcattta agtaatcggg ttgatcttgc ggccccagcg
      301 gtggagtgcg gtaaaacgaa gaaacggcaa gaatgtggct ttcagggata tcgcctaatg
      361 ctttcagggc agcattgacc tgctccagcg gagaggccag attgctgcct atggcaatat
      421 acgccactgt catgcggtac cctcacgacg cggtgcgcgt ttgcgtggac gacgagtacg
      481 gcgacgcggt gacggctctt catccaactc gttaagcatc cctttttgat ctggtggcgc
      541 ggaaacctgg aactcacccc accatttcac cagacgctgc agttcagcgt tacgttcaac
      601 ttcagctcgc aaggccaaca ggtcataagc cgcacggaac ttaggatgct ccagcagttt
      661 ccatgcgcgt ttaccctgac gtcgggacat acgcaactgc aactgccaga tatcgcgggt
      721 taacgtcgtc agacgtttcg ggattgccag tgaacggcag gcttcgtcca gcacgtcgtt
      781 catcgccagc gcgaaagcgt cgtgataggt caggccgctt tcctgggcga tcttctgtgc
      841 cgtctccagc agtgggtacc agaacatggc ggcaaacagg aacgccgggt tcacgcgcat
      901 atcgttatgg atacgcgtat cggtattctt cagcacctgt tcaatgatcc gctccatcgg
      961 gctgtcgcca ttttccgtga agtagcgggt aatggtcggg aacagcggct ggaacagatg
     1021 atattcacac aacagcttat aggtttcgta accgtagccc gcttgtagca gtttaagcga
     1081 ttcttcaaac aggcgtgccg gtgggatatc gttcagcagg gtagcgaggc gagggatcgg
     1141 ttctgcggtt tccgggctga tgcgcatacc caatttggcg gcaaaacgca ccgcgcgcag
     1201 catacgtacc ggatcttcac ggtagcgtgt ttccgggtta ccaatcagac ggataacgcc
     1261 gtccttcaga tccttcatac cgccaacgta atcacggacg gtaaaatccg ctacgctgta
     1321 atacaggctg ttgatagtga aatcgcggcg ctgggcgtct tcttcgatgg agccgaaaat
     1381 gttgtcgcgc agcaacatgc cgttttgccc gcgttgggag gtcgtgcggt cgctgacgtt
     1441 accttcgtgg tgtccacgga aggtcgcaac ttcgataatc tccgggccaa acatcacatg
     1501 agccagacgg aaacggcgac ccaccaggcg gcagttacgg aacagtttgc gcacctgctc
     1561 aggcgtggcg ttagtggtta cgtcaaagtc tttcggcttt ttgccaagta acaggtcgcg
     1621 cacgccgccg ccaaccagcc aggcttcgta tcccgcttta ttgagcctgt acattacctt
     1681 cagggcattt tcactgatat ctttgcggga aatagcatgc tgctcacgcg ggatcaccgt
     1741 cacctgtgga cgggcgactg cctgttcagc ctcgctttcc tcgcggctta gcaccttgcg
     1801 gcaaaaatta gcgactcggg taaaaatagt acacctcggt agtgtcaaac atcattcagg
     1861 acaaaaaaat agcggctaat catagctcag catgacgcat ttgagaatgt tgaatttaca
     1921 attgccgact caggcacggc ggtcagcgtc cagtttttga cggctgactg aaggatttgt
     1981 tcgacgctga aatcctgcca gtgtgcttct gcctgctgcc ccagaaattg aagtgccgcg
     2041 attagtaccg ggcgtggatc gcctttcggc aacgcaggcg catgattctg cttggaaagt
     2101 ttagcgcctt gtggattaag cgccagcggc agatgaatgt aatctggcac tttccagcca
     2161 aaaagctggt acagcgagat ttgccgcact gtcggttcga tcaaatctgc cccgcggaca
     2221 atttcgctca cgccctggaa gtgatcatcc accaccaccg ccaggttata ggcaaacaaa
     2281 ccatcacggc gatgaataat aaaatcttcc cgtgccagtt tttcgtcggc gtgaataatg
     2341 ccgcgcagct ggtcagtaaa ttgcgtgacc ggatgctgct ggcggatacg tactgcggcg
     2401 ttgtctggtc catgatgcaa ctcccggcaa tgaccgtcgt aaataccgcc aatgctttga
     2461 atacgcgcac gcgtgcaggt gcagtaataa cttagtcctt gttcatgtaa ccaggcgagt
     2521 gcttcacgat aggcatcgtg acgttgcgat tgccagagaa catcgccgtc ccagtgcaga
     2581 ccgtaatgtt ccagctggcg caggatagtt tctgcggcac cgggaacttc acgaggcggg
     2641 tcgatatctt caatacgtac cagccagcga ccgtgccggg cgcgagcctg caaatagctg
     2701 ccaagcgcag caatcagaga accaaaatga agctcgccgg aaggagaggg ggcgaagcgg
     2761 ccaatatact gtgtgtctgt catctctttg aacaaaaaat aaggcgggag catttcccgc
     2821 ctgtggtaaa cgtgatggaa cggctgtaat tagccagcca tctgtttttc gcgaatttca
     2881 gccagcgttt tgcagtcgat gcacagatcg gcagtcgggc gcgcttccag acggcgaata
     2941 ccaatttcaa caccgcagga ttcgcagtag ccgaaatctt cgtcttccac ttttttcagc
     3001 gtcttctcga tctttttgat cagcttacgc tcgcgatcgc ggttacgcag ttcgaggctg
     3061 aactcttctt cctgggctgc acggtctacc gggtccggga agttggctgc ttcatcctgc
     3121 atatgtgtaa cggtgcgatc gacttcatcc ctgagttgat tacgccatgc ttccagaata
     3181 cgacggaagt gcgccagctg ggcttcattc atatactctt cgcccggctt ctcctgatat
     3241 ggttccaccc cagcgatggc gagaatactc agggacgatg ttttacggtt ttgcccttct
     3301 tgcatgttgc ttctccttaa cacgcactat cgatccccat gttcggggga aaaatgaggc
     3361 cgctataaat agcagatgct tttccggata gcaattatct aaacgtaaca cttgacaact
     3421 gtgtgaggaa aagcgtattt gcgcacgcga ccagaatgta aattaaccag ttacttactt
     3481 tactacaatg taaccggcag tgatttttta agagccatgc cttcagcaga aatttccgct
     3541 ttgtaagcca gaatttctac ccccctctgt tgagcttctg acaatagttg cgcgtatttc
     3601 tcatcgatgt ggcgcgcggg tgaaaaccgt gtaatggctg aatgcagcac ggcgaaaaag
     3661 ataaccgcac gttggccttc agccgctacg ctcatcaact cccgaaggtg tttctgacct
     3721 cgttcagtga ccgcatcggg aaaatatccc tgttcgttct ccgctaacgt aaccgatttc
     3781 acttcaatat agcagtctgg acgcgaatcc gcctgcaaca taaagtcaat acggctgcgt
     3841 tctgcgccgt attttacttc gcttttcagc gagctatagc ctgacagttc tgaaattgat
     3901 tcattaagga tagcctcttt cgtcaacctg ttagcccaaa gcgtgttgac gcaaataaat
     3961 gcgccgctct ggctttgagt taattcccag gtgtgtgggt atttccgttt ggtgttgtct
     4021 gaaatcgaat accagacggt atcgccaggc gttgcacaac cggtcatcgc acccgtattt
     4081 gggcagtgta gcgttaattc gcgaccatcg ggtgtgatca catcggctaa aaaacgtttg
     4141 taacgctgaa ttagcgtcgc gcgctgtaga gggggagaaa attccattgc gacaatcctt
     4201 gttattgcgt tagcgcccag cgttttagcg gcgtgtagcg tgtgcgtcca cgggcaaacg
     4261 aggaggcgta aagggtgaac tccgtcaccg catacgacca gttaaaacct ggcggcggga
     4321 ttgtcaccgc ctcgctggcg tcgcgcaata aggtaatatg tggatgaaac ggacgattgc
     4381 tttgaaaaca accgctgcgg gcagcctgtg aacggagcat attcgccagc tggattaagc
     4441 cgcgtggcgg ctgacgcatc cctaaccaca ccacacgcga acgcagccat tgtccggcgt
     4501 catcaagcgt gagtgtgaaa ccaggttgac gaatccgtcc ggctaaaaga gaaagcgcct
     4561 tctctttctc ggcgctcact tcgcctaaaa atgccagagt cagatgcaaa ttatcggcgg
     4621 cgaccggacg tcccgcctca ggtgggaagt gtgtggcgcg ccaatggata atctgttcgc
     4681 ggatttctgc aggtaagtcg atagcaaaga acagacgttg cggttcagac atgtgaggca
     4741 ctcggttatg aattaccgcg atgctacaat gtggcgcgaa gaatgttaac cctctggagc
     4801 gttttgtgtc gtcgttgccc gttgctgccg tcttacctga attacttacc gctctcgatt
     4861 gtgcgccgca ggtattatta agtgcgccga ccggggccgg gaaatcaacc tggctgccgc
     4921 tgcaactgct ggcgcatccc ggcattaacg ggaaaattat cctgctggag ccgcgtcgtc
     4981 tggcggcgcg taacgtcgcg caacggctgg cggagctgct taacgaaaag ccaggcgata
     5041 ccgttggcta ccggatgcgt gcgcaaaact gcgtcgggcc gaatacccgc ctggaagtgg
     5101 ttaccgaagg cgtgctgacg cgcatgatcc agcgtgaccc ggaactgagc ggtgttggac
     5161 tggtgatcct tgatgaattt catgagcgca gcttgcaggc ggatttggcg ttggcgctgt
     5221 tactcgatgt gcaacaaggt ctgcgtgatg accttaaact gctgattatg tcggctatcc
     5281 tggacaacga ccgcttgcag caaatgctgc cagaagcgcc tgtcgtcatc tcagaagggc
     5341 gctcgtttcc ggttgaacgc cgttatttac cgctgcccgc gcatcagcgt tttgacgatg
     5401 ccgttgcggt agccaccgct gaaatgctgc gtcaggaaag cggatcatta ctgttatttt
     5461 tacctggcgt cggagaaatt cagcgtgtgc aggaacaact ggcttcgcgc atcggcagtg
     5521 atgtattgct ctgcccgctg tatggcgcgt tgtcgctgaa cgatcagcga aaagcgatcc
     5581 tcccggcacc gcaagggatg cgcaaagtgg tgctggcgac caatattgct gaaaccagtt
     5641 taaccattga aggtattcgt ctggtggtgg attgtgccca ggagcgtgtg gcgcgttttg
     5701 atccgcgcac ggggcttacg cgactgatta ctcaacgcgt tagccaggca tccatgacgc
     5761 agcgtgccgg gcgcgccggg cgtctggagc cgggtatcag cctgcattta atcgccaaag
     5821 aacaagcaga acgcgccgcg gcgcaaagtg aaccggagat cttacaaagc gatctttccg
     5881 gtttgctgat ggaattactg caatggggat gcagcgatcc ggcgcagatg agctggctgg
     5941 atcaaccgcc agtagtgaat ctactggccg cgaaacgtct gttacaaatg ctgggggcac
     6001 tggagggtga acggcttagt gcgcaagggc aaaaaatggc agcgctgggt aacgatccgc
     6061 gtttagcggc aatgctggtt agcgcgaaga acgacgacga agctgctacc gcggcaaaaa
     6121 ttgccgccat tctcgaagag ccgccacgga tgggcaatag tgacctgggc gtggcgtttt
     6181 cgcgcaatca accagcctgg cagcaacgta gtcagcaact gttaaaacgc ttaaacgtac
     6241 gtggcggtga ggcagacagt tcgcttatcg cgccgctact tgccggggcg tttgccgatc
     6301 gcattgctcg tcgccgtggg caagatggac gctatcaact ggcaaacggc atgggagcga
     6361 tgctcgatgc caacgacgcg ctaagccgcc acgaatggtt gatcgcaccg ttattattgc
     6421 agggcagcgc ctcgccggat gcgcggattt tactggcgct gccggtcgat attgatgagt
     6481 tagtacaacg ctgcccgcag ctggtacagc agtctgacac tgtggagtgg gatgacgcgc
     6541 aaggtacgct gaaagcctgg cgtcggctgc aaatcggtct gctgacggtg aaagtgcagc
     6601 cgctggcgaa accctcggaa gacgagttgc atcaggcgat gcttaatggc atccgcgata
     6661 aaggtttaag cgtgctcaac tggacggcgg aagcggaaca gctacgcttg cgtttgttat
     6721 gcgccgcaaa gtggttgccg gaatatgact ggccagcggt tgatgatgaa agtttattgg
     6781 cgacgctgga aacgtggctg ctgccacata tgactggcgt acattcacta cgcggcctga
     6841 aatcactcga catttatcag gcactacgcg gattacttga ttggggaatg cagcaacgtc
     6901 tggatagtga attgcctgcg cattacactg tgccgacggg aagccggatc gccattcgtt
     6961 atcatgaaga taacccgccc gcgctggcgg tgagaatgca agagatgttt ggcgaggcca
     7021 ccaatccgac gatcgcccag gggcgcgtgc cgctggtgct ggagttgctt tcacctgccc
     7081 aaaggccatt acaaatcaca cgagatttga gcgccttctg gaaaggagcg taccgtgagg
     7141 tgcaaaaaga gatgaaaggg cgttatccca aacatgtctg gccggacgac ccggcaaata
     7201 ctgcaccgac gcgacggacg aaaaagtatt cgtaaggttg gttttc
//
LOCUS       DAZKIV010000189         7172 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZKIV010000189 DAZKIV010000000
VERSION     DAZKIV010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.189"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(181..2964)
                     /locus_tag="ACHOT6_004239"
     CDS             complement(181..2964)
                     /locus_tag="ACHOT6_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFP9949355.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQRLRKNQ
                     DAFWEQMVNNEVAAKRALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGQEQKLQIIAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIAQFTA
                     ADALAADRQLFSSPADITFVIVGNVAEEKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKHYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(3021..5393)
                     /locus_tag="ACHOT6_004240"
     CDS             complement(3021..5393)
                     /locus_tag="ACHOT6_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFP9949356.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYISESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5431..7116)
                     /locus_tag="ACHOT6_004241"
     CDS             complement(5431..7116)
                     /locus_tag="ACHOT6_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFP9949357.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMIIG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVADASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
BASE COUNT         1862 a         1638 c         1602 g         2070 t
ORIGIN      
        1 gtgttttatg ctgttatttt tatgcgcatt ctgtgtctcc tgaattatca cgtaaaaatc
       61 agaccttaaa atatcactat tagtacttgg ttattatttt gaacgcattt ataaaattat
      121 tacataaaaa tagcgaatat tgctaaaatc cccgccaacg atgtgttgac ggggctgtta
      181 ttattttggc aataatactc cggtataagt atttaccgga tgagaaagat attgtttaac
      241 ggcagtgtta acattctcta ccgtcatttg tttcaacaat tgctcctgct cagtccatgc
      301 tgcaggatcg tcatattgaa taagactatt tacaatagtg ttcgctaatt gttgaacgct
      361 acgctgttgg atatcgaggc tgcgctgaac gttttgctgg tattcattca gttcttgctc
      421 actgatccct ttagccagac gcttaaccat cacttcattc gctaacgtta acagttcatc
      481 atgtcgttct ggttgacaag taaaagccag caaatgactg atatctttgg cctgaggatc
      541 aaccgagagg cgagaagaaa cgctgtatgc cccagatgcc tgttcacgaa tattaacgcg
      601 tagatctttt gccagtgcga cgttaaaagc atcgagcgcc atacgcgtcg ccagattaac
      661 aggtgtccgg gaatcataat gcttccactg tgaaacctgt gccacaggtt cattttgttc
      721 ttttacagta accgatgcgt tgtccgtcgc gcgagttaat ggtttacctg cggctaatgg
      781 cgaatcagag tgtttgattg atcctaagta acgcgtaatt aacgccacga gtttttcttc
      841 tgcgacatta ccgacaatga caaacgtgat atccgctgga gatgaaaaca actgacgatc
      901 ggcagccagc gcatctgcgg cagtaaactg tgcaatctga ttttcttgca gtaatttcgt
      961 gcggtcatca gcatagcgcg tctcatacat ctgctgggcg aatttctccg ctggacgctg
     1021 gtcgagcgtt ttcaacgcct gaatttgagc attttgtagc gatgcccaaa tattatcgtt
     1081 aatcgtgctg tgggtgattc gctggttaat caactgaaaa ccaggttcag ggttattagt
     1141 ccgcgcgcta acggagagca acgtattcat gccactgact ttactgctca tggttaccga
     1201 attttccgca ctccagcgtt tcaggctgga agaggagagt tcgccaacgc cgcttccgct
     1261 tactgcttta tttgccagcg cgataagtga tttttgctgc gcagggaaac ttaaatcgcc
     1321 tttattcgat acggcaataa tttgtagctt ttgctcttga cccgcggatt ttgccagaat
     1381 aaccctggca ccattggaaa gtgttaatga tgtcagattc tccgccagcg tttctttgct
     1441 gctaatttcc gcctgtgggt cagcgtctac tgttaacgat aaatttctgc cagggaagat
     1501 gtaagccgcc agctttttgt tggcgtactc tttttccagc gccaggatag ccgcaggaga
     1561 caatgctctt ttggcagcaa cctcattgtt taccatttgc tcccaaaatg cgtcctggtt
     1621 ctttcttaac cgctgccatt tttccgccag actttgcacg gtaatttgct gccacagacg
     1681 tttcgaaagt tgatatgtct cttccggcga caagaacggc gtattattta atgagctgga
     1741 tgccaggcga ctggtcagca tacgtaaatc acgttcagct tgctgatcaa ccgcattttt
     1801 cagccaggtg aggcgggtag atttgacatc atcgagttct tcagcagaaa aaccatgctg
     1861 atcaatggtt gccaactctg ccattaatgc attcgcagca tcctgcatat tatcgtctcg
     1921 tgcatttaca cggaaaaaca gcgactgata atcgggtgca attttaacgc tacgcgcagt
     1981 gccgccagaa atagtcttca actcgcccga ctgtatgcgt tcctgcagac gttgattgaa
     2041 cagctgaact aacatgctcc attcagcttg ttcgataaag ctttgctcat cgttcacttg
     2101 taccattggc aggcgataat agagtgcgat gccgttcacc cggttttctt tatcattaat
     2161 gatattaaag cgcaggtgat tttcggcttt tgtcggccag acgcgatttt cagctgcttt
     2221 gttagccgga agcttactta aattatcctt tatcagcgcc agcgcttctt tactgtcgat
     2281 atcgccgacc acgataaagg tcatattatt tggttgatac cagcgttgat aaaattgacg
     2341 caattgtgcc ggtgtgaccg tggcgacagt atccatcagg ccgataggtt cacggtctaa
     2401 attacgggta tttgccagca ggaaagggcg gcgcgcctga gaggtgcgcc atttcgcatc
     2461 ctgatgggca cgccattcct cagtaattac gccgcgttca gcgtctactt cgagtttttc
     2521 aaaggttgcg gcattactcc attcactgaa gattgccatc acttgttgca gattttgttt
     2581 ctgcgtagtc ggcagactca cctgatacac cgtttcgtca tagctggtat aggcattaac
     2641 atcgcgacca aaacgcaggc ccattgactc aaatgtttcg atgactttat tacccggcca
     2701 tgtttttgtg ccgttaaaca tcatatgttc tacaaaatga gccacgccgc gctcattgtc
     2761 ttcttcctgc aatgaaccgg tatgaatttg cagccataaa tttacctgat cctttggttg
     2821 agcatgcgga taaatcatat atcgcaagcc attgtccagt tgcccggtaa ttaacttttc
     2881 atcctgcggt aaggcggcgg caatcagccg cccggggagc aacagagttg ccactaacgt
     2941 cagtaagaaa cagaggtttc tcataattat ctccatgcga aaaccgggcg aatttacccg
     3001 gttaagtaaa atccgaacta ttaaaatttc atgctgacat caagccagaa agtacggcca
     3061 ctggcgtagg tcgccacacc ggtattcgtt gtatcaacag cggttttgct atccagtaca
     3121 ttgagaatat cggcactgat cgtcaggttt tgttgtttca gaaattgtgg cgtccaggac
     3181 aagcgggtgt cccaggtcag gctgctatcc agtttttcgt caacatactg cttgtaatct
     3241 gaatattcgc tgatgtattg cgcatttgtc ttacccagga taatgcgagc tttacgcgct
     3301 tcttgccagg ccagcgtatt cgcccacact aacccgctcg gttgatgcgt gaaatccatg
     3361 tttaaggaga tctttaatgg gttattaaaa tctgccactg gaacgctatc gtaagagacc
     3421 agattaccgt tataaaccac ctggttatca ccggtattgc tctcctcata accattgttt
     3481 aacgacaagt tgcccttgct cttgatatag ctaaagacaa tttgtgggtt aatatctacc
     3541 tggcggatat gcaggggttc ggccagctca aaactgaggt tgaacgaatg cgttttggtt
     3601 ttgccgtcgt tgttatattc agtaatggtg gttttagtcg cgctgtcggt acgactgctt
     3661 ttgctgattt gatcatgcgc ttcacggtaa acatagtttg cacgcgcaat aacgttctta
     3721 ccgattttct gctgcaatcc cattgccagt tcatcgttat aaggtgtttt caaatcctga
     3781 taacgcgtca gggttttatt acctgatacc gattccgtcc agctattgcg gatatcacgt
     3841 aatcccatat caagaatatt cccgccatag taacggttat aaccggcggt aatcattgag
     3901 gtttgatcag caaaaatatc ccattccgtc ataaagcgcg gggagatatt gtggtttgac
     3961 agatagttgt catagtcata acgcacaccg ggcattaacg acacattccg ccagctaatg
     4021 tgatccgcca tatacagcgt gtagttgtca attcccaggc tgcctttacc tttatggtaa
     4081 atggtatggt tagttttctt tcctgcagca ttaatcacat aggattcaga ctggttatgg
     4141 cgttcagtcc acgcatcgga atagatgtat tccgcgccga agtagggttg atgcgaaaca
     4201 ttacccacgg cgaatttttg ccagtccagg cgtgttttga aggtgtaatt atctacagcc
     4261 tgggaaatgt gtcctaatcc gccacgggtg caacgccctg taatatcacc atatgtacat
     4321 gaaagttcgg tgtaccagat gtcatgatcg tgacgggtat aatcactaat atgatcccaa
     4381 ccaacggtgg tacgaagttt ggcccatgcg agctgcgtat ccatatccca tgccagacca
     4441 taggatttat tacccatttc gcgatcagac tgcgggaagg tgctggtatt ataatcacgg
     4501 ctggagccgg tatattttaa ggttaaatcg tgggtaaagc ggtcgctggc aaaccaggta
     4561 aatttgctca atgcagtatc gataacgttt ttatactgtg cccgaccggc gacaatgccg
     4621 tcattcgaaa cataatccgc gcgggtgata tcagactggc ggcgcgataa accggcggta
     4681 acgccgaagt tatcagcgag ttcctgatta aacgacaagg tataaaagtt ctttttaaaa
     4741 tctggggagt aataagtact tcctgaagag ccttgattaa atgcgctctt gttattctca
     4801 tcgatatgcg atgttaacca gtccgaacgc gtagtgcgat aacccagttt cacgctgcta
     4861 tcatcagcgt tgaagcgttt gatctttgca tcaattaccc cgccattgaa gcgaccgaat
     4921 tcaaccggca caaaactgtc ataaagcgtc acattgtcga gtaagctgac atcaagataa
     4981 tacccctgtg acatcccgct aatattggtt gcactactgg catcggactc attcgctggg
     5041 ttcaggttat tagtggcact aataccgtca atcaaatagg cattctgata gggcgacgca
     5101 ccgtgaatag agattttctc cgggcgaata tctccctggt tcaacgaggt actttgcgtt
     5161 gaatccatgc gtaccgcagg gttggttctc agcagatcgc tgatattgcc attcccggtc
     5221 ggtaaacgct cgatgctttc actgatgtaa tgcgtgttgc cgtttacggg tacagggaca
     5281 ggagcataga ccgtcatctc ttcaaaaaca tgcatgtaca tgtttttgtc atcgacggcc
     5341 tgcgccacat cagcgccaca taaaatgacg ccaggaataa gaactcgctt catttattta
     5401 tatctccgta cagcagggct tttattgttt ttataaaacc gcgctaatgt cacaaatatc
     5461 atcggccagg ttccagacac cgggttgatg tgtaaccata atgacgccgc ttgtgggtag
     5521 tttttcacgc actaaacgca gtaagcggat agcctcttgt tcctcaagat gagaggtagt
     5581 ttcgtcaaga aatatccatt tcggacgtcg taaaattaat cgtgccaggg cgatacgttg
     5641 tttttcgccg ctggaaagaa tatctcccca gcgatcgtgg tcatgaatac gcgcagccaa
     5701 tttcccaaga ccaacctgat gcagtacttc gctcaacgat ttatcgtcta cgggcagggg
     5761 aagtgcttta caaataatct ctttcagtaa gccggttttg attaacggtg tttgtgacac
     5821 ataccaactg tcagcaggag aagaaatatc acctttaaac cacggccagc agtgggataa
     5881 tgttttaagc agtgtggttt ttcccgcacc agagtagcct ttcagtaata gccatttgcc
     5941 tggcgaaaca tgaaagttca agttctctaa tatgatctta ttatcagggg tacgaatact
     6001 cgcgtcagcc acttgtaccg catgttggca atttttaggc ttattcgtag ggcgctgttc
     6061 ggtgagttga tggaactcat acaagcgatc gataaccgca gccagttcag caagttcatc
     6121 atatttataa ataaaccagc ttaaattgtt cgataccagc ataaatgcct ggcgcgattt
     6181 catcagtcca cccagattga tctgcccgct aataaactgc ggcaataaca gaaagtacgg
     6241 aagaacgcta agcgagcgcg aataaatatt ctgccagtaa tcaagccacc gctgacgatt
     6301 cattaaacga tgccagttct ctttaatggt atgaaaattg tcgctcaact cctggcgttg
     6361 tagagactca gcattgctta gcgcaatcag ttctgcctgc ttattatgct gcacaagatt
     6421 agttcgaaat gtcgcttcgc tacgttgttt ttccacatta agcggacgaa tacgcttacc
     6481 aaccttatgg gtaaataaag ttccaccgat cacaatgagc acaacggtat agaccatata
     6541 cccctggata ttccattctg ttccaccaac agtgaatgag agcgtacccg cgctttgcca
     6601 aagaataacg gtaaaggtga tcagcatgct aagtgactgg atgaagccaa atgaaagact
     6661 gagcgttttg ctgatcagta agagaatgtc ttcagcgata cgttggtcag ggttatctgt
     6721 atttttatgc tcgccgtaga tctgcgtgaa gtagtagttt ttatctgcaa accagcgatt
     6781 aaggtagtaa tctgttaacc attcgcgcca gcgaatagtc agcagtttaa ttaaccatgt
     6841 tttgttcacg gatattaata caaagatccc cagcagggca gggaaccaaa ggacaagctg
     6901 ccaaagctta tcggtttctt tctggcttag cgcattgaaa aaatcattat tccagtcgtt
     6961 taaccagacc tgaattttaa ccacgccaat gatcatggcg aggataataa tgattaacaa
     7021 cactgaagtt ttattattct tacgcagcca aaagggcttc agcagacaga gatactttgc
     7081 tatcaacata caaaacgtaa tgggaatggt tatcattagc gaaaattgat tgtgccatcc
     7141 aatgattatc tatggcaatg atttactgat ga
//
LOCUS       DAZKIV010000190         7164 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZKIV010000190 DAZKIV010000000
VERSION     DAZKIV010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.191"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..516
                     /locus_tag="ACHOT6_004242"
     CDS             <1..516
                     /locus_tag="ACHOT6_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HFP9949358.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            518..3514
                     /gene="ehxA"
                     /locus_tag="ACHOT6_004243"
     CDS             518..3514
                     /gene="ehxA"
                     /locus_tag="ACHOT6_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFP9949359.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGRTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARMISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIDNLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            3564..5684
                     /gene="ehxB"
                     /locus_tag="ACHOT6_004244"
                     /gene_synonym="hlyB"
     CDS             3564..5684
                     /gene="ehxB"
                     /locus_tag="ACHOT6_004244"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFP9949360.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            5688..7127
                     /gene="ehxD"
                     /locus_tag="ACHOT6_004245"
     CDS             5688..7127
                     /gene="ehxD"
                     /locus_tag="ACHOT6_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFP9949361.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
BASE COUNT         2331 a         1109 c         1602 g         2122 t
ORIGIN      
        1 atgaaatcta atgcttttga cgtcatgggg aaggttgcat ggctatgggc ctgttctcct
       61 ctgcataaaa aatggccatt atctgttttt gctattaatg tcatacctgc tattcagaca
      121 aatcaatttg ctctcttaat aaaggatgag cttcctgtag ctttttgtag ctgggccagt
      181 ttagatctgg aatgtgaggt aaaatatata aatgatgtaa catctttgta tgcaaaagac
      241 tggatgtcgg gagaaagaaa gtggtttatt gactggatag ctccatttgg acacaacatg
      301 gagttatata aatacatgag gaaaaagtat ccttatgagt tattcagagc aataaggtta
      361 gatgagagca gtaaaacagg aaaaatagct gaatttcatg gaggggggat agataaaaaa
      421 ctggcatcaa aaatattcag gcaataccat catgaactta tgtctgaagt aaaaaacaga
      481 caagatttta attttaatat tgagaaagaa aactaatatg acagtaaata aaataaagaa
      541 cattttcaat aatgcgacat tgactacaaa atcagcattt aatacagcat catcaagcgt
      601 acgttccgct ggcaaaaaac tcatattatt aatacctgat aattatgaag ctcagggcgt
      661 gggtattaat gagttggtca aagctgctga tgagcttgga atagaaatac accgtactga
      721 acgagatgat acagcgattg caaaccagtt ttttggtgca gcagaaaaag ttgtaggatt
      781 aactgaacgt ggtgttgcaa tattcgcacc acaacttgac aaacttctgc agaagtatca
      841 gaaagttggg agtaaaatag gaagaaccgc tgaaaatgta ggtaataatc tgggaaaagc
      901 cggaacagtt ctctcagcac tacagaattt tacggggatt gctttatcag gcatggctct
      961 tgatgaattg ctgagaaaac aacgggaagg agaggatata agtcagaatg atattgccaa
     1021 aagtagtatt gaacttatta atcagcttgt agatacagta tcaagtataa acagtaccgt
     1081 tgattcattt tctgagcagc ttaaccagct tggctcattt ttatccagta aacctcgctt
     1141 aagttctgtt ggtgggaaat tacaaaattt accagacctg ggctccctgg gggatgggct
     1201 ggatgttgtc tccggaattc tttctgctgt atcagcaagc tttattctgg gaaacagtga
     1261 cgcacataca ggaacaaaag ctgcagcggg tatcgaactg acaactcagg ttcttggaaa
     1321 tgttggtaaa gctgtttcgc aatatattct ggctcagaga atggcacagg ggttatcgac
     1381 aacagctgca agtgcgggtc tgatcacatc ggctgttatg ctggctatca gtcctctttc
     1441 tttcctggct gctgcagata aatttgagcg agctaagcag cttgaatcat attctgaacg
     1501 atttaaaaaa ttgaattatg aaggggatgc tttactcgca gcctttcata aagaaaccgg
     1561 agctatagat gcagccctga caacaataaa tactgtcctg agttctgtat ctgcgggagt
     1621 tagtgcagcc tccagtgcat ccctcatagg ggccccgata agcatgctgg tgagtgcatt
     1681 aaccggtacg atatctggca ttctggaagc atcaaaacag gctatgtttg agcacgttgc
     1741 agagaaattc gctgctcgga tcaatgaatg ggaaaaggag catggcaaaa attattttga
     1801 gaatggctat gacgcaagac atgctgcgtt tttagaagac tctctgtctt tgcttgctga
     1861 tttttctcgt cagcatgcag tagaaagagc agtcgcaata acccagcaac attgggatga
     1921 gaagatcggt gaacttgcag gcataacccg taatgctgat cgcagtcaga gtggtaaggc
     1981 atatattaat tatctggaaa atggagggct tttagaggct caaccgaagg agtttacaca
     2041 acaagtgttt gatcctcaaa aagggaccat agacctttca acaggtaatg tatcaagtgt
     2101 tttgacattt ataacaccaa catttacccc aggagaagaa gttagagaaa gaaaacagag
     2161 tggtaaatat gaatatatga catctcttat tgtaaatggt aaggatacat ggtctgtaaa
     2221 aggcataaaa aatcataaag gtgtatatga ttattcaaaa ttgattcagt ttgttgaaaa
     2281 gaataacaaa cactatcagg cgagaatgat ttctgaactc ggagataaag acgatgtggt
     2341 ttattctgga gcaggctcat cagaagtatt tgctggtgaa ggtcatgata ccgtatctta
     2401 taataagacg gatgttggta aactaacaat tgatgcaaca ggagcatcaa aacctggtga
     2461 gtatatagtt tcaaaaaata tgtatggtga cgtgaaggta ttgcaggaag tcgttaagga
     2521 acaggaggtg tcagtaggga agcgaacaga gaaaatacaa tatcgtgatt ttgaattcag
     2581 aaccggtgga attccttatg atgtaataga taatcttcat tctgttgaag agctcattgg
     2641 cggaaaacat gatgatgaat tcaaaggcgg taagtttaat gatatattcc atggcgcaga
     2701 tgggaacgat tatatcgaag gtaattatgg taatgatcga ctatacggcg atgatgggga
     2761 tgattatata tccggaggac agggagacga ccagttattt ggtggtagtg gaaacgataa
     2821 attgagtgga ggggatggta ataattatct gacaggagga agcggtaatg atgagcttca
     2881 ggcacacgga gcttataata ttctgtcagg tggtactggt gatgataaac tttatggtgg
     2941 tggtggtatt gatcttctgg atggagggga aggtaatgac tatctgaatg gtggttttgg
     3001 taatgatatt tatgtttata ggcaaaacta tggtcatcat acaattgcag atgaaggagg
     3061 taaaggagat cgtttgcact tatctgatat tagctttgat gatatcgcat ttaagagagt
     3121 tggaaatgat cttatcatga ataaagccat taatggtgta ctttcattta atgagtcaaa
     3181 tgatgtcaat gggataacat ttaaaaactg gtttgcgaaa gatgcctcag gagcagataa
     3241 tcatcttgtt gaggttataa cagataaaga tggtcgagag ataaaagttg ataaaatacc
     3301 tcataataat aatgaacggt caggttatat aaaagccagt aatatagcat ctgaaaaaaa
     3361 catggttaat atcaccagtg ttgccaatga tattaataag attatttctt cagtttcagg
     3421 gttcgattca ggtgatgaac gattagcatc tttatataat ttatccttac atcaaaacaa
     3481 cacacactca acaactttaa cgacaactgt ctgagactaa aagaggaggt gatggccatt
     3541 ccaggaggaa tggtcatcgt tatatgatga gtaaatgtag ttctcataat agtctgtatg
     3601 cactgatatt gcttgcacaa tatcataata taactgtcaa tgctgaaact ataaggcatc
     3661 agtataatac ccacacacaa gattttgggg tgactgaatg gttactggca gcgaaatcca
     3721 ttggcttaaa agcaaaatat gtagaaaaac atttttccag attgtcaata atttctttac
     3781 ctgcgttgat atggcgggat gacggtaagc attatatatt gtctcgtgtt actaaagatt
     3841 catcacgcta tcttgtttat gatccagaac aacatcagtc actaactttt agtcgggatg
     3901 agtttgaaaa actgtatcag ggaaaagtca ttctggttac gtcaagagca acagtagtcg
     3961 gagagttagc taaatttgat ttttcttggt ttatcccctc tgttgtgaaa tacaggagga
     4021 ttttacttga ggtgttaact gtttctgctt ttattcagtt tcttgcgtta ataacacctc
     4081 ttttttttca ggttgtaatg gataaggttt tagttcaccg ggggttttca acgttaaata
     4141 ttatcacaat agcatttatt atagtgatac tttttgaagt gatattaacc ggagccagaa
     4201 cttatatttt ctctcatact acaagtcgta ttgacgtcga actgagcgct aagttattca
     4261 gacatttgct tgcattgcct gtttcatatt ttgaaaatcg cagggtcgga gagaccgttg
     4321 ccagagtaag ggaactggag caaattcgta attttttaac cggacaagcg ttgacatcag
     4381 ttcttgatct atttttttct gtaatatttt tttgtgtcat gtggtattac agccctcaat
     4441 taacactggt tatattattg tcactacctt gttatgttat atggtcattg tttatatcac
     4501 ccttattacg tcgacgtctt gatgataagt ttctcaggaa tgcagaaaat caagcttttc
     4561 ttgtcgaaac ggtaacagca ataaatacaa ttaaatccat ggcagtatca ccacagatga
     4621 ttgctacatg ggataaacaa ctggccgggt atgttgcttc cagtttcagg gtaaatctgg
     4681 ttgcaatgac agggcagcag gggatacagc tgatacagaa aagtgtaatg gtaattagtc
     4741 tatggatggg agcacatctt gtcatatcgg gagagataag catcgggcag ttaatcgcgt
     4801 ttaatatgct tgcaggtcag gtcattgctc ctgttatcag actggctcat ctttggcagg
     4861 attttcagca ggttggtatt tctgttgagc gccttggtga tgtattaaat acaccggtag
     4921 agaaaaagtc aggcagaaat atactgccgg aaattcaggg ggatatcgaa tttaaaaatg
     4981 tcagatttcg gtattcttct gacggtaatg ttattttgaa taatattaat ttatacatat
     5041 caaaggggga tgttatcggt atagttggcc gttctggttc aggaaaaagt acattaacaa
     5101 aactgcttca gcgcttttat ataccagaga ccggacagat tttaattgat gggcatgatt
     5161 tatcacttgc agatccagaa tggttacgac gccagattgg tgttgtattg caggaaaata
     5221 tactattaaa tcgtagtatt atcgataata ttacattagc ttctccggct gtatctatgg
     5281 aacaggctat tgaggcagcc agacttgcag gtgcccatga ttttattaca gaactaaaag
     5341 aagggtacaa tactattgtt ggagaacagg gcgttggtct ttcaggaggg cagcgccaac
     5401 ggatcgcaat agcccgggct cttgttacaa atcctcgaat tcttattttt gatgaagcaa
     5461 ccagtgctct tgattatgag tcggaaaata taataatgaa aaatatgtca agaatatgta
     5521 agaacagaac cgtaattatt attgcacaca ggttgtcaac tgtaaaaaat gcaaacagaa
     5581 taattgttat ggataatggc tttatttctg aagatggcac acataaagag cttatctcca
     5641 aaaaagacag tttatatgca tatttatatc agttgcaggc ataacgtatg cgcttttaca
     5701 tgaaaggttt atgggatttg gtttgccgtt ataagacggt tttttctgat gtctggaaaa
     5761 ttcgtcacac acttgatgct cctgtgagag aaaaagatga gtatgcattt cttcctgctc
     5821 accttgaact cattgaaaca ccagtatcca gacgttctca ttttgttgta tggagtattt
     5881 tattatttgt aattatatct cttctcttat ctgttcttgg gaaagttgaa gtggtttcag
     5941 tagcaaatgg taagtttact catagtggaa gaagcaaaga aataaaaccg atagagaatg
     6001 cgattgtaga aaaaataatg gtaaaagatg gctcttttgt aaaaaaaaat gatccattag
     6061 ttgaattgac ggtgcctggt gttgaatctg atattttaaa atcagaagca tctttgttgt
     6121 atgaaaaaac agaacaatat cgctacgcaa ttctttctga atcaatacaa cggaatgagc
     6181 ttcctgaaat aaggataaca gattttcctg gcggagaaga taacgcggga ggtgaacatt
     6241 ttcagagggt tagctcatta ataaaagagc aattcatgac atggcagaac agaaaaaatc
     6301 aaaagcagct cacattaaat aaaaaaatag ttgaacggga tgcagcgctt gctcgtgtta
     6361 gcctgtatga gcatcaggta tcacaggaag gaagaaaact caatgatttt aagtatttgt
     6421 tgaataaaaa agctgtttct caacattcag ttatggagca ggaaaatagc tatattcagg
     6481 caaaaaatga acatgcagtc tggcttgcac aggtttctca acttgaaaaa gaaatagaac
     6541 ttgtgcggga agaactggca ctggagacga atatcttcag aagtgaaatt atcgagaagc
     6601 acagaaaatc aacagataac attgtgttgc tggagcatga gcttgaaaaa aacaggcaga
     6661 gaaaagcatc atcttttatt aaagctcctg tgagtggtac tgttcaggag ttaaatatac
     6721 atacagaagg tggtgttgtc acaacagcag aaacgctgat gattattgtc cctgataatg
     6781 atattctcga agtaacagca tctgtactta acaaggatat cggttttata caacctggac
     6841 aggaggtcgt tattaaagta gatgcatatc catatacacg tcatggttac cttacaggga
     6901 aagtaaaaaa tataactgca gattctgttt ctgttccgga tacagggctt gtatttaacg
     6961 tgattatatc tgttgatcgg aatgatatac agggagaaag aaaaaaaatc cctgttacag
     7021 ctggaatgac tgttatggca gaaataaaaa ccggggtgcg tagtgttatc agctatcttc
     7081 ttagtccatt aaaggaaact attaatgaaa gtttacgtga acgttagtat ataaataaca
     7141 tattggcttt ttatatgata taga
//
LOCUS       DAZKIV010000191         7154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZKIV010000191 DAZKIV010000000
VERSION     DAZKIV010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.192"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..204)
                     /locus_tag="ACHOT6_004246"
                     /pseudo
     CDS             complement(1..204)
                     /locus_tag="ACHOT6_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(195..759)
                     /locus_tag="ACHOT6_004247"
                     /pseudo
     CDS             complement(195..759)
                     /locus_tag="ACHOT6_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(924..1883)
                     /locus_tag="ACHOT6_004248"
     CDS             complement(924..1883)
                     /locus_tag="ACHOT6_004248"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFP9949362.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGW
                     FRHQDMAEGYLVMKSLDQEICSRDRSKMGDNVWCCITAQASHS"
     gene            1893..2126
                     /locus_tag="ACHOT6_004249"
     CDS             1893..2126
                     /locus_tag="ACHOT6_004249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949363.1"
                     /translation="MSQQPVRLRVSGGIPQVIKCRTVTEVNGGGIFQREEQRMCLTCP
                     CSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            complement(2058..2261)
                     /locus_tag="ACHOT6_004250"
     CDS             complement(2058..2261)
                     /locus_tag="ACHOT6_004250"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFP9949364.1"
                     /translation="MVLSDCYSLANEQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            2439..2612
                     /locus_tag="ACHOT6_004251"
     CDS             2439..2612
                     /locus_tag="ACHOT6_004251"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949365.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            3185..3734
                     /locus_tag="ACHOT6_004252"
                     /pseudo
     CDS             3185..3734
                     /locus_tag="ACHOT6_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            3911..4660
                     /locus_tag="ACHOT6_004253"
                     /pseudo
     CDS             3911..4660
                     /locus_tag="ACHOT6_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(4665..6035)
                     /locus_tag="ACHOT6_004254"
                     /pseudo
     CDS             complement(4665..6035)
                     /locus_tag="ACHOT6_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6055..6402)
                     /gene="tnpB"
                     /locus_tag="ACHOT6_004255"
     CDS             complement(6055..6402)
                     /gene="tnpB"
                     /locus_tag="ACHOT6_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9949366.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRDRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(6399..7073)
                     /gene="tnpA"
                     /locus_tag="ACHOT6_004256"
     CDS             complement(6399..7073)
                     /gene="tnpA"
                     /locus_tag="ACHOT6_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9949367.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTKPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT         1710 a         1650 c         1829 g         1965 t
ORIGIN      
        1 tttcgtagtc tatctttcct gaattactac aaaaaactca ctccaatatg atgctatatg
       61 tcgacaccat atgatttccc cattgcaatc actaaccttt ccatttagat ttatactggc
      121 atctctcttt tttgataaat aagggggatt gttttttgaa atatattcaa cataatttga
      181 tgccgatttg tttattattg gcatatgttt gtatcctttc tataaatttt ccgcctgaca
      241 gtggaaataa tatattctac tgatttttgt agtttaaacg atttatgaag aattttactg
      301 ttgtacagta ccagatactc agccagtttc agattgaaca tattcagcta ttcaaagagc
      361 aattcttcat taaactcaat aaattgttcc ctcagtgttc ggttaaaatg ctcacttatg
      421 gcatttaatt ttcggcgtat agagataagt ccatacgaac ccgacggtgg cttcctgtag
      481 cgttttgtcg aattttcccg agaactcttt gcccatcatc agtgatgatc tggttgatac
      541 caacaaggaa caactgggct acacagcaga agaaatgcct gatgatatca cttttaagcg
      601 atagtacggc cagagagtag ttgctgtgtt cgtcgagcat ggtaatgaaa taacggtaca
      661 gctcgccatt ctgagctcaa tagtatccat gccactgagc ttacaggtct ttatcgggcg
      721 atagtgcttt attcttcggg gctttgtgga tgatttttcg aaatgttcat gatagttagg
      781 taaagctacc gcatatcatt tgcaaaatga cagatagata tacttcaatt tgaatcttaa
      841 caattagtat gggaatgctg tcaatcctct gtatgcgtcc accacatatt gaaatttttg
      901 ttctctaata tattccacgg gcgtcaagag tgtgaggcct gtgctgttat acagcaccag
      961 acgttgtccc ccatctttga cctgtctctt gagcagatct cctgatcaag agacttcatc
     1021 accaggtaac cctcagccat atcctgatgt ctgaaccagc catcccacat aactacccaa
     1081 ccggggctgc ttgtgcgttt gctgtcatgc catcgcccca gtttagccag tttcaaacag
     1141 gcccatttca gggtcggtgt ttgcgccgga agctgctttc cttccagctt aacccacagt
     1201 agtttccact ctgtcggcgt cagtattttc tcacagctgt cattttgtgt ttcttcactg
     1261 atacctccct gccgcaggcc cagcacccgc accgcgataa ccaccatgcg ctcaaggtta
     1321 tcccgggtct gcattcgcag cgattccaca catgtaccac cacttttcca cgctttgtgg
     1381 tattcctcta tcagccagcg ttgctcgtaa tggctgacga tacgtcgcgc atcggcggca
     1441 ctcgccactt tttctgatgt cagcggatac cagcaggctc cgtcctctcc tgctccccgc
     1501 aacagacata cgtgagcgag agcgcctggc cgctgttgtc gggatttttt atgctgactt
     1561 cgctgtaact gatgagcatc cgggcctggc gggctgcccg cccgcctttt tgcatcacat
     1621 tcagcgtgtg gcttcccgcg gctgacttta taatgcaggt aatgccagat atccgcttcc
     1681 cggtcacaga cagtgattac ctgtttctgt atctccccca gccgttcggc catgcgctcc
     1741 gaagcctgct gccagcggta actttctttt tcttcacagg gacgttcttt tcgctggtgc
     1801 ttaacaccgt aggtgtccgt ggcacgactc cagcactgct gttcgataag atcgactggc
     1861 agggcgctgt cgggggcgta catcaggaca gagtgagcca gcagcccgtg cgtcttcggg
     1921 ttagtggtgg tattccccag gtcatcaaat gccgtactgt gaccgaagtt aatggtggtg
     1981 gtatcttcca gcgcgaggag cagcggatgt gcctcacatg cccttgcagt ggcagtaaat
     2041 ccggcttcgg caatggctta cggggacacc gacgggttac gtatcaggcg gtacgcacct
     2101 tcaacctgag cagtgaagtg ggatgatttc acaatagaaa gacctgcatg ctgagcgaga
     2161 gaagaggtca gtgacacaag gcgtcgtgta cgacgcggat caccgagacg ggcatgtcca
     2221 gactgctcgt tagccagtga ataacaatca gaaagtacca taacagaccc gaagaaaatg
     2281 aaatataaga gaagatcaac gggtgaagaa aaagttcaaa aaatcgctgc cggggaggaa
     2341 ggaaactacc ggatggaaag agtcccccta aagcagactg acagacatca caaatccccg
     2401 ggtggggatt tgtgtataag agacaggtta caggtgtggt gaataagact gcaaatgtgg
     2461 cagcccatgc cgtggtgaat gctgcactgg cggtggtaca gggaaataac gcccgtgcca
     2521 gtgccgcagg tgctgccact ggagacgtgg tggggatgat agccacagag atgtgcggaa
     2581 tatcccctgg cactgttaca tgtaaaagat aaataacgaa gctacatcct tctctttatg
     2641 aacggcttta tattagtttt gctgacaaga ttgagctaaa tccggccaat gaagaagagc
     2701 aggttattct ttaaaggttt gataacatgt aacacatttt gaatattcag tcagtaagcc
     2761 ttaaaaattt acctggttat gggctgactg atataacaaa gtaaatattc gatggggaat
     2821 gccggatgca atgaacaatg cattaacgta tcgaaataat gatgcaggag caaactcagt
     2881 ctggtgagta tacattatgc cattgatgca gagtttaaag gaacagggct ggttcgttat
     2941 ggaacaacat ttgtgcctga ggaaaggggg gggagtatct gaggcaacag aagtttgtgg
     3001 ggagttcggt ttattaccat gcttggtttt ttgtcagtgt gttcaaatcg tcaggtgata
     3061 tatttatagt tttttgatga tgatttcaaa taatgttttt gagatgtgtg gtcctttttg
     3121 attttcagaa atcatttatt aatgaaggag ggttaaatga aaaaaactcg ttataatttc
     3181 agtaatgata ttatctgtta tgagtggttc aagactggct gataatcata cgctatcggc
     3241 tggttatgct cagagtaagg tacaggactt taagaatatt aaaggagtga atctgcaata
     3301 tcgatatgag tgggattctc cggtcagtgt cgtcgggagt ttttcgtata tgaaaggtga
     3361 ttgggcggac agtcatcgcg atgaagctga cgatttttac agacatcagg ctgatattaa
     3421 atattattca tttctcgcag gcccagctta tcgtctgaat gactatatca gtttttatgg
     3481 tttggtgggg atatctcata ctaaagcgaa gggagattat gagtggagaa acagtgtggg
     3541 tgctgatgag tctgatggat atttaagtga gtctgtatca aaaaaaatcc actgattttg
     3601 cttatgcagc tggtgttatt attaatccgt ggggcaatat gtctgtaaat gttgggtatg
     3661 agggaactaa agcagatata tatggaaaac attcggtcaa tggatttact gttggagttg
     3721 gttatcgttt ttaatcatta gacgggactg gataattgca cttgcgtagt gaagtttact
     3781 gaatgtttgt attttaatga agtattcgac atattccctt cagacggaat atgtcgacgg
     3841 ttatggtttt actggagcat gagtatgtta tggcgaaact gataaatata tcaggtccac
     3901 tgaggagaga atgacgccag gggagaggag ggtggcttca cgactggaga gctttctgaa
     3961 tgatgattgc tttgtctggt acgatatccc ggtgggacgg aaaaaccgac atccggattt
     4021 tgtcattatt gacccggata acgggctggt atttctggag gtcaaggact ggactgtatc
     4081 cacattacgt aaagcaaatc aggagcaggt tacactggaa accgacggtc ttctgaaatc
     4141 agaaataaat ccactggtac aggttcgtcg ttatgcctgt gatacggtta atgccttacc
     4201 tgctgacccg tgcttacggc agaatgacgg tcagtataag gggcggctga acctggcctg
     4261 ggcgtatggt gtggtgttta cccgcattac ccgtcagcaa ctgaaggcac tgacagggaa
     4321 taatgagaat gcggtggaaa agatttttcc ttcagcacag acaatctgtc aggatgaaat
     4381 gacgtagtct gttttacctg aggtattccg gcaaaaaatt gccgggatgt ttaccacggg
     4441 gttcagaacc cgggtaacac cccggatgag ggatattctg cgcgcccatc tgtttcctga
     4501 agtgaccgtg aaacagaact cacaaatcaa aattatggat attcagcagg agattctggc
     4561 cagaaatatt ggtgacgggc atcgtgtcat tcatggtgtg gccggttccg gaaaaaccat
     4621 gattcttttg ttccgttgcc tttacctggc agaaaccacg tccggtagtt acgccgcccc
     4681 agtgcaacta cccggagggc gttttcacac aggttattgt cgatctccac ccagccattg
     4741 cggcagtatt cgttcagagc gtcccattgc ttcagcagat aggtgaacgc tttcgctgta
     4801 tccgagtggc gcgacagcgt gctcatctgc ccctggagcc actcatacaa cgactgcatt
     4861 agcggtaccg ttctggcttt tctgaccgcc agtcgctctt ctgccggact gccgcggatc
     4921 tcagcctcga tagcgtacag ttccccgata cgacggagcg cttctcccgt tactgtcgtt
     4981 ggatggcgga catgtacatc gtggatcttg cgtcgggcat gtgccataca agccgcttcg
     5041 gttacctgac cgctttcgta aagagcattg taacccgcat atgcatcggc ctgcaggata
     5101 cctctgtagt ccgccagatg ttgctgtggg tggatgcctt tgcggtcggg agagtatgag
     5161 aaccacaccg ctgccggcat ggtcgaaccg gcgttgcgat cgtcacgaac atagacccac
     5221 agccgtccgg tacgggtttt accctgaccc ggctccagta catttaccgg cgtgtcatcg
     5281 gcatgcactt tacccggcat cagcacatag tgcttcagtt catcatacag cgggcgaagc
     5341 tgctctccca tgatgtcaac ccagcgcccc atcgtattgc ggtgcagctc cacgccctgg
     5401 cgggcataga tttctgactg acggtacagc ggcagatgct cggcgaactt agccatgatt
     5461 atgcgggcca gcagagccgg actggcgtaa ctgcgctcga tgggttttgg tggctgcgga
     5521 gcctgaacta tacagtcgca ccggctgcag gcccgttttg ggcgaaccgt ttcgattacc
     5581 ctgaacgcgg tgttgatgat atccagttgt tcagagatgc tttctcccag cggtttcagt
     5641 ttgccgctgc agacggggca ttcggtttct gccggggaga taacctgcct gtcacgggga
     5701 agtgttgccg gaagtgcttt gcggacggga gagtctgatg ttttcggcgc tgtctctccg
     5761 gccattgagg tgagttgcaa ctgcgcctca ccaagcatgt tctggagctc ggttatacgc
     5821 gtttctgccc gtgcgatctt cttttctatc ttctcgcggc ttttctcgct gctgcgaccg
     5881 aacaacattc tctgcagttt agcgaccagc gctctgagtg agctgatctc gcgggcatag
     5941 ccggttattt caccagacag acggacgata acagcctgct gtgcgatcag cagggccttc
     6001 agttgctcga tgtcgtcggg gagtgtgttg ttcattcccc tgttttatca cgggttatat
     6061 ccggatgcca ggccgttctg tccgtttggg atgttgccat gcgatcccct ccagtagcat
     6121 ggataactga gctggcgtca ggtgcacttt cccttcccgg gttaccggcc agacgaagcg
     6181 gccccgttcc aggcgtttgg cgaacaggca taacccgtca cgatcggccc acagtatttt
     6241 caccattttg ccactgcggt cccggaagac gaagatatgc ccggagaacg ggtcatcttt
     6301 cagcgtgttc tgcaccttcg aagccaggcc gttgaagcca caacgcatat ctgtgatgcc
     6361 agcgatgatc cagattctgg taccggttgg cagcgttatc atcgggtacc tcctttcatt
     6421 tcgcggatta gcgcccgtaa catttccgga gtgagagggt caaacagttt taccacacct
     6481 gatttaagat gcagctcgca ccgtgggacg tttccgggat cactctcagg gcactcatca
     6541 ggcttgttac gccagaaggg atttgtaact ggtctggtcg gctctggcgt atcagtcaga
     6601 gccaccggga caggcatgca ttcctgtatg tcatcatcgc tcagtaagcc gtcctcgtac
     6661 tggcttttcc atttaaacag caggttatca ttgataccgt gctctctggc gatccgggca
     6721 acaacagcac cgggctgtaa tgcctgctta gccagacgga ccttaaattc acggctgtag
     6781 ctggctcgcc gttcttttcg ccatgtgcct tcgctgattt gaggctttgt taattccttc
     6841 tttctgttgg cataaaggat ggcgtcaagc tgagctaatg aaactgaatc gggcaatggc
     6901 catgcgatac cggatgcaat aaatcgctga aaaagcgtat gtattgtgga atgactgaga
     6961 cctagacgct gagcgatggc ccggatggtc agtttatctt caaatcttaa acgcagagca
     7021 tcaggcaaat aagaacggaa gcagggaata tctttttttg tctgggaatt catcgttcgt
     7081 gtccatctat atagatgggc gcgattgttg ccagacagga caattttcac aagacgtcgc
     7141 agatggggcg ctta
//
LOCUS       DAZKIV010000192         7130 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZKIV010000192 DAZKIV010000000
VERSION     DAZKIV010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.193"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..886
                     /gene="hlyE"
                     /locus_tag="ACHOT6_004257"
     CDS             <1..886
                     /gene="hlyE"
                     /locus_tag="ACHOT6_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HFP9949368.1"
                     /translation="VEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELSRFKQE
                     YSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYIFLFDEYNEKKASA
                     QKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSSYFQSQ
                     VDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQNFFTTLSN
                     TVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKKMINTC
                     NEYQKRHGKKTLFEVPEV"
     gene            complement(1093..1539)
                     /gene="ycgN"
                     /locus_tag="ACHOT6_004258"
     CDS             complement(1093..1539)
                     /gene="ycgN"
                     /locus_tag="ACHOT6_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFP9949369.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1631..2290)
                     /gene="ycgM"
                     /locus_tag="ACHOT6_004259"
     CDS             complement(1631..2290)
                     /gene="ycgM"
                     /locus_tag="ACHOT6_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFP9949370.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2362..2655)
                     /locus_tag="ACHOT6_004260"
     CDS             complement(2362..2655)
                     /locus_tag="ACHOT6_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFP9949371.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPLEDLLKQHLSVMGQKTDDTNK"
     gene            2893..3294
                     /gene="pliG"
                     /locus_tag="ACHOT6_004261"
     CDS             2893..3294
                     /gene="pliG"
                     /locus_tag="ACHOT6_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFP9949372.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3414..3782)
                     /gene="ycgJ"
                     /locus_tag="ACHOT6_004262"
     CDS             complement(3414..3782)
                     /gene="ycgJ"
                     /locus_tag="ACHOT6_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFP9949373.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4302..4997
                     /gene="minC"
                     /locus_tag="ACHOT6_004263"
     CDS             4302..4997
                     /gene="minC"
                     /locus_tag="ACHOT6_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFP9949374.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5021..5833
                     /gene="minD"
                     /locus_tag="ACHOT6_004264"
     CDS             5021..5833
                     /gene="minD"
                     /locus_tag="ACHOT6_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFP9949375.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5837..6103
                     /gene="minE"
                     /locus_tag="ACHOT6_004265"
     CDS             5837..6103
                     /gene="minE"
                     /locus_tag="ACHOT6_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFP9949376.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6475..6818)
                     /locus_tag="ACHOT6_004266"
                     /pseudo
     CDS             complement(6475..6818)
                     /locus_tag="ACHOT6_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6853..6972)
                     /locus_tag="ACHOT6_004267"
     CDS             complement(6853..6972)
                     /locus_tag="ACHOT6_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFP9949377.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAV"
     gene            complement(6933..7118)
                     /gene="ymgJ"
                     /locus_tag="ACHOT6_004268"
     CDS             complement(6933..7118)
                     /gene="ymgJ"
                     /locus_tag="ACHOT6_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFP9949378.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
BASE COUNT         2038 a         1692 c         1572 g         1828 t
ORIGIN      
        1 ggtagaagta gttaaaaacg caatcgaaac tgcagatgga gcattagatc tttataataa
       61 atatctcgat caggtcatcc cctggcagac ctttgatgaa accataaaag agttaagtcg
      121 ctttaaacag gagtattcac aggcagcctc cgttttagtc ggcgatatta aaaccttact
      181 tatggatagc caggataagt attttgaagc aacccaaaca gtgtatgaat ggtgtggtgt
      241 tgcgacgcaa ttgctcgcag cgtatatttt tctatttgat gagtacaatg agaagaaagc
      301 atccgcccag aaagacattc tcattaaggt actggatgac ggcatcacga agctgaatga
      361 agcgcaaaaa tccctgctgg taagctcaca aagtttcaac aacgcttccg ggaaactgct
      421 ggcgttagat agccagttaa ccaatgattt ttcagaaaaa agcagctatt tccagtcaca
      481 ggtagataaa atcaggaagg aagcgtatgc cggtgccgca gccggtgtcg tcgccggtcc
      541 atttggatta atcatttcct attctattgc tgcgggcgta gttgaaggga aactgattcc
      601 agaattgaag aacaagttaa aatctgtgca gaatttcttt accaccctgt ctaacacggt
      661 taaacaagcg aataaagata tcgatgccgc caaattgaaa ttaaccaccg aaatagccgc
      721 catcggggag ataaaaacgg aaactgaaac aaccagattc tatgttgatt atgatgattt
      781 aatgctttct ttgctaaaag aagcggcaaa aaaaatgatt aacacctgta atgagtatca
      841 gaaaagacac ggtaaaaaga cactctttga ggtacctgaa gtctgataag cgattattct
      901 ctccatgtac tcaaggtata aggtttatca catttaaaaa cacagacgtt ttttaaagat
      961 tgagagttac ctgttgataa taaggtatcg ggtaactctc attatgacag agcgctttca
     1021 aaaacagggc tacgaaatgg cgagcatcca gacgtatatt aagaaagccc ccatcaacat
     1081 ctattaaatt tatcactgcg cccagtcagg tttatttaag atatgatcct gccagtcaat
     1141 cacttctgat tctttcactg cgatatgacg cacagagata cgttcaccat gcattgccgc
     1201 tttcgaacca gtaagtagcg gatgccacgc aggtaaatct ttaccttccg ccagcaaacg
     1261 ataagcgcag gtcattggca gccattcgaa tgttggcaga ttttcacggg ttaatttaat
     1321 gcagtcgggt tcaaactcga aacgacgttc gtagttccga cattgacagg ttttaatatt
     1381 gagctggcga caggcgacgt tagtgaagta gatttcgtcg gtgtcttcat ccatcagttt
     1441 atgcaggcaa cactgaccgc aaccatcaca caacgactcc cattccgcat cgctcatttc
     1501 gtccagggtt ttactttgcc agaaaggtac atcgctcatc aggtgttccg ccattacgtt
     1561 aaaacgcacc ttataaccag tctggcacag cgatgcaagt tttgccgccg ctttcaggcg
     1621 gcaaaaagta ttacaaaacg cgagttgtca aagaatgccc atcgaaagtg actgtcagct
     1681 catcaccgct ttgcaacggg ccgacgccat caggcgtgcc tgtcaacaca acgtcaccgg
     1741 ccttgagggt aaaaaacttg ctcatataag cgatcaacgg aacgatttta tggatcatgt
     1801 ccgcagtcgt accttgctgg cgttgttcgc cgtttacgct caggcccagc gttgtatttt
     1861 gtggatcgcc ggtaaattcc gccgcgggga taaacccgga aagcggacaa gagttatcaa
     1921 acgctttagc cttttcccac ggctgcccgg ctttcttcat ttttccctga acatcacgca
     1981 atgtcagatc gagcgccacg ccgtaaccgg caatggcttt gcggacatgc tcttccgttg
     2041 cctgacgcag cgtcgcgcca atcaacaccg ccagttcgac ttcatgatga actgaaccga
     2101 aatcggatgg gatcgccaat ggctgccgca gatcgcacag tgccgtttct ggtttaataa
     2161 acagcactgg ctcttcgggc actgcgctgc ccatctcttt aatatgtttg gcatagttac
     2221 tgccaacaca gactacttta ctcaccggat aatccagcag cgcaccttgc cagttgtgat
     2281 gttgatacat aacgttcccc tcaccttatc gtggatatga aaacccgcgc cgacaaagat
     2341 aatgccaccg ccggatatcg gttatttgtt agtgtcgtct gttttctgcc ccatgacgga
     2401 aagatgttgc ttcagcaaat cttcgagtgg tggcggtaac tgcaaatagt aaccttgctc
     2461 ggttaatgcc agtttaacct tttcaatatc agcattgacc agtttcttcc gcccatccaa
     2521 cggcagaatc atcgctaact gaggctgacc aaaacctttc atcagttcct caggaacacg
     2581 cgaaaaatcg tctttttttt cgacatataa ataggtctgg tcacgcttgc tgcttcgata
     2641 aatcacacaa aacatacttt tactctttaa gatgccaggt tttggcataa gctaacatca
     2701 atgaaacccg cgccacaagc cagctgtaga tttctgcggt ttgttgagtc catgcccctt
     2761 tataagtaat ccccccggca aaaaatgccg cgccgggatt ttaccgaacg cagaaaagcc
     2821 actggaattc tcccgtaagg gattattata ttataacagt tcatcgtact cattctgaac
     2881 aggagactac caatgaaaat caagagcatc agtaaggctg tattgctgtt tgcactgcta
     2941 acctccacga gctttgctgc gggtaagaat gtcaatgttg agttcagaaa aggccatagc
     3001 agcgctcagt actccggcga aataaaggga tacgattacg atacatatac cttctatgcc
     3061 aaaaaaggtc agaaagtaca tgtgagtatc tctaatgaag gcgctgatac ttaccttttc
     3121 gggccaggaa tcgatgattc tgttgattta tccagatatt ccccagaact cgatagtcac
     3181 ggacaatact cactccctgc ttctggcaaa tatgagctgc gagtgctgca aacccgaaac
     3241 gacgcccgta aaaacaaaac taaaaaatat aacgtcgata ttcaaattaa gtaaatgtca
     3301 accgacgggg actctccccg tcataaacgt taacgcccca ctcctctctg tcgctcaaga
     3361 ggttaatgac gaatagcaga caatgccgaa tagaacataa tgtaaacaag gcatcactca
     3421 cgacacataa ataacatctt tgtggtgatt tgattgattt ttccactccg cttaccatcc
     3481 acaccaaaat aacgatcatc acgacataat ttttctttaa catcgcaaaa tacaccgtta
     3541 gcgaatgtaa attcgctggg atcgtagcct tgtaatgatt gtaagttttc agcggctttt
     3601 tcgccgagat atttccttgt taactctggg gaaatgccat ctgagttcac acaaatatac
     3661 gggtcgcaaa caacgccgca cgccggagat tgaagtccgc cagcatcatg tccagacgcc
     3721 attgatgaga acatcatccc cacacctgac aatccgatat aaaaaatacg catcatattc
     3781 atagcacatc ctgataatcc aaccgtacaa cacttatgct catttcatca tcaatccgta
     3841 atcaaaaccg ctgacctgga cgagttacga tgacctgaca gaagtatcag acgtcttctg
     3901 cggtttctat gatagagcaa ttctgtggcg gatattaaga taacgggtat ctgttcttta
     3961 atgatgctgc aaaaaatgga aagttaagaa agcctggcgt ggtgaaaaaa tacacttgcc
     4021 tcaatactgg atatttaaac agttctggag atgctgtcac gattaggatg cggaaaaagg
     4081 aagcgtaaat aagaagaatc aaaagtacgg cggcaatggg ttgattgaca agggtatttt
     4141 ttaagctatg aatcagcgcc atttatcaca gaatagactt ttactctgaa taaatgggag
     4201 ggtgacttgc ctcaatataa tccagactat aacatgcctt atagtcttcg gaacatcatc
     4261 gcgcgctggc gatgattaat agctaattga gtaaggccag gatgtcaaac acgccaatcg
     4321 agcttaaagg cagtagcttc actttatctg tggttcatct gcatgaggca gaacctaagg
     4381 ttatccatca ggcgctggaa gacaaaatcg ctcaggcccc cgcattttta aaacatgccc
     4441 ccgttgtact caacgtcagt gcactggaag acccggtaaa ctggtcagcg atgcataagg
     4501 cggtttcggc aaccggtttg cgggttattg gcgtaagtgg ctgcaaagat gcgcaactta
     4561 aagccgaaat tgaaaagatg gggctgccta tcctgacgga aggaaaggaa aaagcgccgc
     4621 gtccagctcc cgcaccacag gctccagcgc aaaatacaac gccggtcaca aaaacgcgtt
     4681 taatagatac cccggtgcgt tccggtcagc gtatttatgc tccacaatgt gatctgattg
     4741 ttacaagcca cgttagcgct ggggccgaat tgattgccga tgggaacatt catgtctatg
     4801 gcatgatgcg cggtcgtgcg cttgcagggg caagtggtga ccgggaaacg caaatatttt
     4861 gtacgaacct gatggcggaa ctggtgtcca tcgcaggtga atactggctg agtgatcaaa
     4921 tcccagcaga attttatggc aaagcggcgc gactgcagtt agtcgaaaac gctttgaccg
     4981 ttcaaccgtt aaattgatcc ctttttaaca aggaatttct atggcacgca ttattgttgt
     5041 tacttcgggc aaagggggtg ttggtaagac aacctccagc gcggccatcg ccactggttt
     5101 ggcccagaag ggaaagaaaa ctgtcgtgat agattttgat atcggcctgc gtaatctcga
     5161 cctgattatg ggttgtgaac gccgggtcgt ttacgatttc gtcaacgtca ttcagggcga
     5221 tgcaacgcta aatcaggcgt taattaaaga taagcgtact gaaaatctct atattcttcc
     5281 ggcatcgcaa acacgcgata aagatgccct cacccgtgaa ggggtcgcca aagttcttga
     5341 tgatctgaaa gcgatggatt ttgaatttat cgtttgtgac tccccggcag ggattgaaac
     5401 cggtgcgtta atggcactct attttgcaga cgaagccatt attaccacca acccggaagt
     5461 ctcctcagta cgcgactctg accgtatttt aggcattctg gcgtcgaaat cacgccgcgc
     5521 agaaaatggc gaagagccta ttaaagagca cctgctgtta acgcgctata acccaggccg
     5581 cgtaagcaga ggtgacatgc tgagcatgga agatgtgctg gagatcctgc gcatcaaact
     5641 cgtcggcgtg atcccagagg atcaatcagt attgcgcgcc tctaaccagg gtgaaccggt
     5701 cattctcgac attaacgccg atgcgggtaa agcctacgca gataccgtag aacgtctgtt
     5761 gggagaagaa cgtcctttcc gcttcattga agaagagaag aaaggcttcc tcaaacgctt
     5821 gttcggagga taagttatgg cattactcga tttctttctc tcgcggaaga aaaacacagc
     5881 caacattgca aaagaacggc tgcagattat tgttgctgaa cgccgtcgca gcgatgcaga
     5941 accgcattat ctgccgcagt tgcgtaaaga tattcttgag gtcatttgta aatacgtaca
     6001 aattgatcct gagatggtaa ccgtacagct tgagcaaaaa gatggcgata tttctattct
     6061 tgagctgaac gtgaccttac cggaagcaga agagctgaaa taagcccgct gtaaaaacgc
     6121 atttatcttc aaggcagagt catttctgcc ttgagttttt catccctctc atccacgttg
     6181 tggtaaagcg gcgagtattc ttgctgatac tcctcattgc tatttccacc tcccccttta
     6241 caccttaagg ctgttcccac gcacagaaca aaatgtttat gcctggttga gtaaataacc
     6301 tcattgttag tgtggataca ctgggggatg ataaccccgc aatttcatta ttcattgttc
     6361 tcccatagga tgaggtttcc cgccgtaact ggcgggcttt ttttgcccca gaattttccc
     6421 ttttagcccc ctgtaaacga aaactgcgcc gaagcgcagt ttaatcgttg atggtcagaa
     6481 cgaccagttc acaccagcca ccgcattcca cggggattcc acaccggcac catggctata
     6541 ccccaccccg agatgccgct taacgtactg ctgaatgagg ctttaatacc tgcctggtat
     6601 attccacgtc tgcccgacaa atcattgacg aaattaccgt cattattcac cttcacccgg
     6661 ttatcatcga caaattcttt gcgcacagcc gccttcagcc acggctcaat ttccataccg
     6721 ttccccagac gcatgttgta actcagcgtt gcacccagtt cacgatatat actgcgggta
     6781 tcgactgatt tcgattccat gccattggat aaatgatagt agcgtctaag tgacctaagc
     6841 tctgctgcaa catcagaccg caggcaccag accaggggat tcatcctgaa gagacagcgc
     6901 aggtgtattg tgttcaccgc tcaccaaaga catcatgatg aaatgatgat atcccgcata
     6961 agaaagaggc attttttaaa cgcagtgcgc tgaagtgtag ttggataaaa aatcactctt
     7021 ttcagataat actgtcgtac gcttatcttt cgacaatgag gccgtcggca aaataaaatg
     7081 actaatgtaa tcgttgctga tgaatatctt ctgctcacat aaaaatcaca
//
LOCUS       DAZKIV010000193         7128 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZKIV010000193 DAZKIV010000000
VERSION     DAZKIV010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.194"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..314
                     /locus_tag="ACHOT6_004269"
     CDS             135..314
                     /locus_tag="ACHOT6_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9949379.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            304..1245
                     /locus_tag="ACHOT6_004270"
     CDS             304..1245
                     /locus_tag="ACHOT6_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949380.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTAEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWKKIIALYEQAAECDGEVVR
                     PKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRVSRDPFWCRNVLSPSKLREKWDELS
                     LRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAGFRG"
     gene            1242..1736
                     /locus_tag="ACHOT6_004271"
     CDS             1242..1736
                     /locus_tag="ACHOT6_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9949381.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLFRRAATVWMEAFRESHSQPERNNFLAHRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            1736..2389
                     /locus_tag="ACHOT6_004272"
     CDS             1736..2389
                     /locus_tag="ACHOT6_004272"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFP9949382.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            2386..2712
                     /locus_tag="ACHOT6_004273"
     CDS             2386..2712
                     /locus_tag="ACHOT6_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFP9949383.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLVGEENARLRAAHWLH
                     ERGLKV"
     gene            2709..3098
                     /locus_tag="ACHOT6_004274"
     CDS             2709..3098
                     /locus_tag="ACHOT6_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9949384.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            3118..3915
                     /locus_tag="ACHOT6_004275"
     CDS             3118..3915
                     /locus_tag="ACHOT6_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFP9949385.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRVAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNIEPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            3923..4912
                     /locus_tag="ACHOT6_004276"
     CDS             3923..4912
                     /locus_tag="ACHOT6_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9949386.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRHRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            4926..5678
                     /locus_tag="ACHOT6_004277"
     CDS             4926..5678
                     /locus_tag="ACHOT6_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9949387.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKICGRCNGKRFSRLPTTLARHHVQKLVPDLSDYQ
                     WYKGYANVIDKLVTKCWQEESYAEAQLRKVTR"
     gene            complement(5957..6046)
                     /locus_tag="ACHOT6_004278"
     CDS             complement(5957..6046)
                     /locus_tag="ACHOT6_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949388.1"
                     /translation="MNKPVCLDDWLIGFKSLCCTLTLIALLIM"
     gene            complement(6101..6313)
                     /gene="cspF"
                     /locus_tag="ACHOT6_004279"
     CDS             complement(6101..6313)
                     /gene="cspF"
                     /locus_tag="ACHOT6_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspF"
                     /protein_id="HFP9949389.1"
                     /translation="MSRKMTGILKTFDGKSGKGLITPSDGRIDVQLHVSALNLRDAEE
                     ITTGLRVEFCRINGLRGPSAANVYLS"
     gene            6614..6829
                     /gene="cspB"
                     /locus_tag="ACHOT6_004280"
     CDS             6614..6829
                     /gene="cspB"
                     /locus_tag="ACHOT6_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspB"
                     /protein_id="HFP9949390.1"
                     /translation="MSNKMTGLVKWFNADKGFGFISPVDGSKDVFVHFSAIQNDNYRT
                     LFEGQKVTFSVESGAKGPAAANVIITD"
BASE COUNT         1837 a         1579 c         1967 g         1745 t
ORIGIN      
        1 cttcctgtac cgggtggaaa agcgaaggtg gagtattgct atgcagtgaa tgtaccaggt
       61 gacagggaaa ttgtaaccca ctgctttgca gagtgggctg tgggtgattt caaccggcag
      121 aaggagacag tcctttgcga caagttaacc gctggttcaa agatcactac ggagtgcccg
      181 tcagagtcat tcgttgggaa ccggaaacac aacgggttat ctacctccgc gaaggttatg
      241 agcatgaatg cttcagtccg ctcgaacagt ttcgtcgtaa attcagggaa atagaggtcg
      301 gtcatgagca ctaaattaac cggctatgta tgggatggtt gcgctgcatc aggcatgaag
      361 ttatccagcg tggcaattat ggcccgcctg gctgatttca gtaatgacga aggtgtgtgc
      421 tggccatcaa ttgaaaccat tgcccgtcag attggcgcgg ggatgagtac cgtcagaacg
      481 gctatcgcac ggctggaagc agaaggctgg ttaacgcgta aggcgcgtcg ccagggtaac
      541 cgcaatgcgt cgaatgttta tcagcttaac gttgcgaagc ttcaggcagc ggcattttct
      601 caactgtcag attctgaccc gtcaaaatct gacgcatcaa aatctgaccc gtcaaaattt
      661 gatgcgtcga aatctggcaa aaaagcgggt tttcacccgt cagaatctgg cggggatccg
      721 tcagtaaaat caaaacatga tccgtcagat aaaaaacctt ctcgtccgga cgcttcgcaa
      781 ccggacacgc agacggctga acaggatttt ttaactcgcc atcctgatgc ggttgtattc
      841 agccctaaaa agcgccagtg ggggacgcag gatgatttga cctgcgcaca gtggctctgg
      901 aaaaaaatca tcgccctgta cgagcaggct gccgaatgtg acggcgaggt ggttcgtccc
      961 aaagaaccga actggacagc ctgggcaaac gaaattcgcc tgatgtgtgt gcaggatggt
     1021 cgtactcaca aacaaatctg cgagatgtac agccgcgtca gccgcgatcc gttctggtgc
     1081 cgtaacgtgc tcagcccgtc gaagttgcgg gaaaaatggg atgagctttc cctgcgctta
     1141 tcgccgtccg tcagcacgta caccgaaaaa cgcgaagacc cgtacttcaa atccagttac
     1201 gacaacgtgg actacagcca gatcccggca ggattcaggg ggtgatcatg agtctgttaa
     1261 atgacgttca gaaattcatt gaagcccatc cggggtgtac ttccggagac attgcagatg
     1321 cttttgctgg ttactcacgg cagcgcgttc tgcagtcagc aagcaagtta cgtcagagtg
     1381 ggcgtgtggc tcaccgttgt gaaggggata cacgcagaca tttcccgcgc ctgactgaga
     1441 gagcgcagga gccggaacca caaccagttc gtgaaaccag acctgtgcgc aatttctatg
     1501 tcggcactaa cgacccccgg gtgattttgt gcctgacccg ccaggctgaa gaactggagt
     1561 ccaggggctt attccgtcga gctgcaacgg tgtggatgga ggcattccgt gaaagccact
     1621 cccagccaga acgaaacaat tttctggcgc atcgtgagcg gtgcttacgg aaaagcagca
     1681 agcgcgctgc atcgggtgaa gagtggtatc tgtcagggaa ttacgtgggg gcttaatgag
     1741 taataaatat tgccaggcgc tggtggagct gcggaacaaa ccagcccatg aactgaagga
     1801 agtgggcgat cagtggcgca cgccggacaa cattttctgg ggaattaaca ccctgtttgg
     1861 cccgtttgtt ctggatctgt tcactgatgg tgataacgcc aaatgtgccg cttattacac
     1921 tgcggaagac aacgcgctgg cgcatgactg gtctgaacgt cttgcggagc ttaaaggtgc
     1981 tgcctttggt aatcccccgt acagccgcgc cagtcagcat gaggggcaat acatcaccgg
     2041 catgcgttac atcatgaaac atgccagtgc tatgcgtgat aaaggcgggc gctatgtttt
     2101 cctgatcaaa gctgccacca gcgaagtttg gtggccggaa gatgcagacc atattgcttt
     2161 tattcgcggg cgtattggtt ttgaactgcc agcctggttt atcccgaagg atgagaagca
     2221 ggttccgaca ggagctttct tcgctggtgc tattgctgtt ttcgacaaga cctggaaggg
     2281 accggcaatc agctacatcg ggcgcgatga acttgaggca tgtggtgagg cctttctggc
     2341 gcaggttcgc cagcaggcag aaaaactggt cagggagatg gtggcatgac gacgttaact
     2401 caatgccagc agcaggtgct ggatatgctg atttcttatc agaaagaacg tggcttcccg
     2461 ccaaccaatc aggaggtggc aaccatgctg ggataccgtt cagtgaatgc agcggtagaa
     2521 catcttcgcg cactggagaa aaaaggcgtc atcacgataa agcgtggcgt ggcccggggc
     2581 atcacgcttc ataccgcggt gaaggacgac gacagcgagg cggtcgggat tatccgctca
     2641 ctgcttgtcg gtgaggaaaa cgccaggctg cgtgcagccc actggttaca tgagaggggc
     2701 ctgaaagtat gaagctgatc ctgcctttcc cgcccagcgt gaacacgtac tggcgacacc
     2761 ccaacaaagg ggcgtttgca ggtaagagcc tgataagcgc ggcggggcgc aaattccaga
     2821 gcgcggcgtg tgcagcaata gttgagcagt tacgtcgtct gccgaaacca acgtcggcac
     2881 ctgcttcagt ggagatcgtg ttgtttcctc cggataacag gatccgcgat ctggacaact
     2941 ataacaaggc gctgtttgac gccctgaccc acgcgggggt gtgggaagac gacagtcagg
     3001 tgaaaagaat gctggtggag tggggaccgg ttatcccgaa agggaaggtc gagatcacta
     3061 tcagtaagta tgagaaaccg gcgggtgcag ccgcctgatt aagaggagaa acgaagtatg
     3121 aataatctga tggttattga tggtattgaa gttcgtcgtg atgcttatgg gcgttacagc
     3181 ctgaacgatc tgcacagggt tgctggttct ctggataagc ataagcctgc attctggctc
     3241 cgcaatgagc aaactgagcg tttaataagc gagttgcaga tttgcaactc ggtcaatata
     3301 gagccagtta acgttattcg tggcggaaat aaccagggga cgtatgtctg caaagaactg
     3361 gtgtatgcct atgcaatgtg gatcagcccg tcattccatc tgaaggtgat ccgtactttc
     3421 gacatggtaa ccagcgcacc ggaaaaatta tccggacagg ctgctgacaa gatgcaggct
     3481 ggtgtgatcc tgctggactt tatgcgccgg gaattaaacc tgtctaactc atcagtgctt
     3541 ggagcctgtc agaagcttca ggaggctgtt ggcttaccga atctggcacc gcgctatgcc
     3601 attgatgctc ctgctgacgc gcctgatggc tcaagccgcc ccacgctgtc actgagtgca
     3661 ctgctgaagc agtatggtat ccgcctgaca gctaatcagg catatcacca gatggtgaag
     3721 ctggggatcg tcgagcagcg cgaacgatac agccgtaccg cgattaacaa catcaaaaaa
     3781 ttctggtcgc tgacagcgaa aggttgcatg ttcggcaaga acatcaccag tcccgcaaat
     3841 ccgcgcgaga cgcagccgca tttcttcgaa tcccgattcc ctgagctgtt aaagctgctc
     3901 gataccgttc attgaggtga ccgtgagagc attactgacc cctgaaattg ccccgcgtat
     3961 ggggatcgta ttgttcagac ccggttcaga gctgatgccc ctgtttatgc aggggcgtgt
     4021 cctgctggag cctgagccag aacgttattc atcttttgcc agtggtgccg ttccggcggc
     4081 atcacaaccg ctggcggatg atcctgccgt tcgggccgtg ttccgcaatg aggcagtgat
     4141 ccgtcgtgct ggtggcgtgg aatgtcttga aagctggtta cttcgtgaaa aaggctgcca
     4201 gtggcctcat tccgactggc acagcgagaa catgacaaca atgcgacacg ctccgggcgc
     4261 aatccgtctg tgctggcact gcgataacca gctgcgcgat cagttcacgg aacggctgga
     4321 atcaatggca acggataact gtgcccgctg ggtgttgtct gttgtgcgtc gggatctcgg
     4381 ttttgatgac agtcatgttg tgacaatgcc ggaactgtgc tggtggctga ttcgtaatga
     4441 tctggcggat gccttaccgg aaagtgcagc ccgtaaggca ctgagattac cgaagcctgt
     4501 tgtgccgtct gttacccggg aaagtgacct tgtgccttcg gttcctgcca ccagcatcat
     4561 ccaggataag gcgaaaaagg tgctggcgct gaaagtggat ccggagtcgc cggagtcttt
     4621 tatgttacgc ccaaaacgtc accgctgggt taatgaaaag tacacgcgct gggttaagac
     4681 gcagccgtgc gcatgttgtg gaaaacctgc tgatgatccc caccacctga taggccacgg
     4741 tcagggggga atgggtacaa aagcgcatga cctttttgtg ttgcctttgt gcagaaagca
     4801 tcacgacgag ctgcatgcgg ataccgtggc atttgaagag aagtatggct cccagctgga
     4861 gctgatattt cgttttatcg atcgtgcgct ggcaataggc gtactggcgt aagtggagaa
     4921 cgagcatgaa ccttgaagcc ttaccaaaat attactcccc aaaatctcca aaattgagtg
     4981 atgacgcacc ggcgacaggc tctggtggtt taacgattac ggatgtgatg gctgcgcagg
     5041 ggatggtgca gtcgaaagca ccgcttgggt ttgccttatt cctggcaaaa gttggtgttc
     5101 aggatcctca gtttgcgatt gaaggtctgc tcaattacgc gatggcactg gataacccga
     5161 cattgaataa attgagtgaa gaaacccggc tacagattat tccttacctt gtgaattttg
     5221 cctttgctga ttattccagg tctgcggcaa gtaaggctcg ttgtgagcat tgtgctggta
     5281 ctggatttca taatgtattg cgcgaagtgg tgaaacactc cagaagcggg gaatctgtta
     5341 tcaaagaaga gtgggtgaag gaactatgtc agcattgcca tggtaaggga gaagtcagca
     5401 cagcgtgcag agggtgtaag ggtaaaggta ttgtcctgga tgaaaaaagg acccggcttc
     5461 atggcacacc tgtttataag atttgtgggc gttgcaatgg aaaacggttt agtcgtttac
     5521 caaccacact ggcgcggcat catgtccaga agctggtacc ggacctgagt gattatcagt
     5581 ggtataaagg atatgcaaat gtcattgata aactggttac aaagtgctgg caggaggaat
     5641 cttacgctga agcacaattg agaaaggtga cgagataagt gattttcgcc gaagatggcg
     5701 acgtgatgct tgcatttttc aaaaaatatg gatataattt tttcaacgat gggctttgta
     5761 tacccgaagt taagaaaaag tagaaaaccc gctgatgagc gggtttttgt gctttaaata
     5821 gggtaataga gatgttgaat ctcattacgg gattcatgtt agtttactta ttatttatcg
     5881 ggtgactttg ttttttgcct gatgtttaaa atgttttctt ccagtacaat gtccatagat
     5941 acaatgagtc tgcttattac attattagca gagctattaa ggtcaaagta cagcataagc
     6001 ttttaaagcc aatcaaccag tcatcaagac agacgggttt attcatgaaa actctccatg
     6061 tttgattcga tggggcttga agttaaagct ttaatatagc tcatgaaagg taaacattgg
     6121 cagctgatgg tccacgcaga ccatttatcc ggcaaaattc cacgcgtaat ccggtggtaa
     6181 tttcttctgc atcgcggaga ttgagcgctg aaacatgaag ctggacatcg atacgaccat
     6241 cggatggggt tataagacct ttgccgcttt tgccgtcaaa ggttttgaga attcctgtca
     6301 ttttacggga caaaaaaatt ccttaatact gataacttgg cgcactatac acacgttcct
     6361 gaagaaagct atagtttttt gatggggttg aagatggctg gatgtctaaa ataaacattg
     6421 cttcatatgt tcaactatgc gttaatgatt gcgtcggttt gaagaacaga cgatatacga
     6481 agtagtttac taaagcagtt ctcatttcag gtgttattca cttattcctt ctttgagtct
     6541 ctccaattaa gtacgaagtc gtttctgtta tacaagccat ttatgccgaa aggctcaagt
     6601 taaggaatgt agaatgtcaa ataaaatgac tggtttagta aaatggttta acgctgataa
     6661 aggttttggc tttatttctc ctgttgatgg tagtaaagat gtgtttgtgc atttttctgc
     6721 gattcagaat gataattatc gaaccttatt tgaaggtcaa aaggttacct tctctgtaga
     6781 gagtggtgct aaaggtccag cagcagcaaa tgtcataatt actgattaaa attcatagtt
     6841 tgtctgtata cgataacgaa gaaggctgat gcctgagtgg agatacagac agagtggtga
     6901 atattggatc tctttaataa atagtaagga ggtccaatac atgaaacaat ggccagcata
     6961 tttggcaata acttaatcag gaaaagtatg ctaaccattg tggtgaagta caggtttgct
     7021 gcatgaatag ttttacagca gaagctaact gctggcatag caaaacaaag tgcgtaagtg
     7081 gatgactccc acaaaaagca ccacaatatt aaacccgctc aggcgggt
//
LOCUS       DAZKIV010000194         7125 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZKIV010000194 DAZKIV010000000
VERSION     DAZKIV010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.195"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..1433
                     /gene="adeQ"
                     /locus_tag="ACHOT6_004281"
                     /gene_synonym="yicO"
     CDS             99..1433
                     /gene="adeQ"
                     /locus_tag="ACHOT6_004281"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFP9949391.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPFSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1486..1938
                     /locus_tag="ACHOT6_004282"
     CDS             1486..1938
                     /locus_tag="ACHOT6_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFP9949392.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2149..3339
                     /gene="nepI"
                     /locus_tag="ACHOT6_004283"
     CDS             2149..3339
                     /gene="nepI"
                     /locus_tag="ACHOT6_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFP9949393.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3380..3673)
                     /gene="yicS"
                     /locus_tag="ACHOT6_004284"
     CDS             complement(3380..3673)
                     /gene="yicS"
                     /locus_tag="ACHOT6_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFP9949394.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3895..4713
                     /gene="nlpA"
                     /locus_tag="ACHOT6_004285"
     CDS             3895..4713
                     /gene="nlpA"
                     /locus_tag="ACHOT6_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFP9949395.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4717..5640)
                     /locus_tag="ACHOT6_004286"
     CDS             complement(4717..5640)
                     /locus_tag="ACHOT6_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFP9949396.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5751..6935)
                     /locus_tag="ACHOT6_004287"
     CDS             complement(5751..6935)
                     /locus_tag="ACHOT6_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFP9949397.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     IAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1796 a         1601 c         1791 g         1937 t
ORIGIN      
        1 cattaatgtg caattaaaat gtgataatta tcactgaacc gttgttgtca tttgttttac
       61 aaaagcaagg gattaaatct cctccaatgg acaaaaaaat gaataatgac aataccgatt
      121 acgtgagtaa tgaatcaggg acgctttcgc gattatttaa actacctcag catgggacca
      181 ccgtccgcac agaattgatt gcggggatga ccactttttt aaccatggtg tacatcgttt
      241 ttgtgaaccc gcaaatcctc ggcgcggcac aaatggaccc gaaagtggtg tttgttacca
      301 cctgtttgat tgccggtatc ggcagtattg cgatggggat atttgctaac ttacccgtgg
      361 cgctggctcc ggcaatgggg ctgaacgcct tctttgcctt cgtggtcgtg ggggcgatgg
      421 gcatctcctg gcagaccggg atgggcgcaa tattctgggg cgcagttgga ctatttttgc
      481 tcacgctgtt tcgtatccgg tactggatga tctccaacat tcccttcagt ttacgtattg
      541 gtatcaccag cggaattgga ttatttatcg ccttaatggg attaaaaaat actggcgtta
      601 ttgtcgccaa taaagacacg ctggtgatga ttggcgattt aagttctcac ggcgtgttgt
      661 taggtatttt agggtttttt attataaccg tgttgtcatc acgtcatttt catgccgcgg
      721 tgctggtttc tattgtggtg acgtcttgct gtggattatt tttcggtgat gttcatttta
      781 gcggcgtcta ttccattccg cctgatatta gcggcgtcat tggtgaagta gatttgagcg
      841 gcgcgttaac acttgaactc gccggtatca ttttctcctt tatgctgatc aacctatttg
      901 attcatcagg aacattaatt ggtgtaactg ataaagcggg cttaatagat agtaacggta
      961 aattccccaa tatgaataag gcgctgtatg ttgatagcgt cagttcggtg gcgggggcgt
     1021 ttatcggcac ctcgtctgtt actgcctata ttgaaagtac ttctggtgtg gcagtcggtg
     1081 gtcgcacggg gctgactgcg gttgtggttg gcgttatgtt cctgttagtt atgttcttct
     1141 caccgctggt ggcgatggtt cctccttacg caaccgccgg agcgttaatc tttgttggcg
     1201 tgctgatgac ttcgagcctg gcgcgcgtta actgggatga ttttaccgaa tcggtgcctg
     1261 cgtttattac cacggtgatg atgcccttta ctttctcgat caccgaaggg attgcactcg
     1321 gctttatgtc gtactgcatc atgaaagtat gcaccgggcg ctggcgcgat ctgaacctgt
     1381 gtgtggtggt ggtcgcagct ctgtttgcac tgaagattat tctggtggat tagtaactgg
     1441 cgagggtttc cagatatcat gagttctgat tacgcaggag aactcatgat ctggataatg
     1501 ctcgccacgc tggcggtagt gtttgtggtt ggttttcggg tgctgacatc cggggccaga
     1561 aaagcgattc gccgtctcag cgatcggctg aatatcgatg tcgtacccgt ggagtcgatg
     1621 gtcgatcaaa tgggaaagtc agccggtgac gaatttttac gttatttgca tcgcccggat
     1681 gagtcgcacc tgcaaaacgc cgcgcaggtg ttgctaatct ggcaaattgt cattgtcgat
     1741 ggtagcgaac agaacctgct gcaatggcat cggattttac aaaaagcccg tcttgccgcg
     1801 ccgattaccg atgctcaggt caggctggcg ctgggttttc tgcgcgaaac cgaacctgaa
     1861 atgcaggata ttaatgcttt tcagatgcgc tataacgcgt tctttcagcc tgccgagggc
     1921 gttcactggt tgcattgaaa ccgtttcgtc ttatcagcct acagaccgcg cctgacctgt
     1981 cgggtggata agacgttcac gccgcatccg gtaatctttg ccctgttccg tttcgatctt
     2041 aactccagct caagcaacac cgtaaaagtt gcaaaaacgt taaatgcgtc acacatttca
     2101 atgttaaagt ggtcggcttt tcccctgaaa catgccacgg gtaacaccat gagtgaattt
     2161 attgccgaaa accgcggcgc ggatgccatc acccgaccga actggtcagc cgttttctcg
     2221 gtggcgtttt gtgtcgcctg tctgattatc gttgagtttt tgcccgtcag tttgttgacg
     2281 ccaatggccc aggatttagg catttcggaa ggggttgccg ggcaatcggt gaccgtgacc
     2341 gcctttgtgg caatgtttgc cagtttgttt attacccaga caattcaggc tactgaccgc
     2401 cgctacgttg ttattttgtt tgccgttttg ctgacgctct cctgcttgct ggtttccttt
     2461 gctaactcat tcagtttgct tttaatcggt cgtgcctgtc tggggctggc gctgggcggg
     2521 ttctgggcga tgtcggcgtc gctgaccatg cgtctggtgc cgccgcgtac ggtgccgaag
     2581 gcgctgtcgg tgatcttcgg cgcggtttct attgcgctgg tgattgccgc gccgttgggc
     2641 agttttttag gcgagcttat cggttggcgc aatgtcttta atgcggcggc ggtgatgggc
     2701 gtgctgtgta ttttctggat tatcaaatca ttgccttcac tgccaggcga accctcgcat
     2761 cagaaacaaa atactttccg cttattacaa cgtccgggtg tgatggcagg gatgatcgcc
     2821 atcttcatgt ctttcgccgg gcagtttgct ttcttcacat atattcgccc ggtgtatatg
     2881 aacctggcgg gattcagcgt ggatggctta acgctggtgc tgttgagttt tggcattgcc
     2941 agctttatcg gtacgtcgct ttcgtcattc attcttaaac gttcggtaaa actggcctta
     3001 gcaggcgcgc cgttaatact ggctgtgagt gcgttggtgc tgacgttgtg gggaagcgat
     3061 aaaatcgttg ctaccggcgt ggcgattatc tgggggctaa cttttgcatt ggttcccgtc
     3121 ggctggtcaa cgtggatcac ccgctcgctg gccgatcagg cagaaaaagc cgggtctatt
     3181 caggtggcgg ttattcagct tgctaatacc tgtggcgcgg caatcggcgg ttatgcgctg
     3241 gataatattg gtctgacttc gccgttgatg ttgtccggca cattgatgtt gctgactgca
     3301 ttgttggtta ctgcaaaggt gaaaatgaag aaatcctgat atcaggtaaa gaagcgttgg
     3361 tcagtagttg ttgctgttct tacatatccg ggcattctac cttacccaac gcttcccaat
     3421 aattgctctg attattacgt tccatcgcca cgatggcctc gcgtatatgt tgtttattgt
     3481 tttcatctga caacattaac ttttcccacg cttcatctga taatgacgcc tgtggcgtgc
     3541 aaattttacg taaatcttgt aataccgtgg ttttctcttt tgcggactgc agtgagctaa
     3601 aaggcgactc ggcgtatacg atgcctggca tagcgaaaaa agtgaaaata agcagtagtg
     3661 tcgttggttt catggattca accttttaca aatgtgtatc gaatgaagca taagtgtagt
     3721 ttgctttcat cgctctcatc atgctgtacg aaacgcgctt gcgtggtcag taagaagtgc
     3781 cagactttat attccacttt tattcctttt tattcttatc gatagcgttt cgttttttaa
     3841 accgcagcga ccttaccgct atagtcaggt aatcattaat aaaaggataa aaaaatgaaa
     3901 ctgacaacac atcatctacg gacaggggcc gcattattgc tggccggaat tctgctggca
     3961 ggttgcgacc agagtagcag cgatgcaaaa cacattaaag ttggcgttat aaatggcgca
     4021 gaacaagatg tcgcggaagt cgctaaaaag gtggcaaaag agaaatatgg cctcgatgtt
     4081 gaactggtag gatttagcgg ttcgttgcta ccgaacgatg cgacaaatca tggcgaactt
     4141 gatgcgaatg tcttccagca tcgacccttt cttgaacaag ataatcaggc gcatggttat
     4201 aaactggtag cggtgggaaa tacgtttgtc ttccctatgg cgggttattc caaaaaaatc
     4261 aaaaccgtgg cgcaaataaa agaaggcgcg acagtggcga tccccaacga cccgaccaac
     4321 cttggtcgcg cacttttact gttgcaaaaa gagaagctaa ttactctgaa agagggaaaa
     4381 gggttattac ctaccgcgct ggatataacc gataatcctc gtcatttgca gattatggaa
     4441 ctcgaggggg cgcagctccc gcgcgtactg gatgatccca aagttgatgt agcgattatc
     4501 agcaccactt acattcagca gacagggctt tctccggtgc acgacagcgt atttattgaa
     4561 gataagaatt cgccgtatgt gaatattttg gtggcacggg aagataataa gaatgcagaa
     4621 aacgtgaagg aatttctgca atcttatcaa tcacccgaag tcgctaaagc ggcagaaacc
     4681 atttttaacg gtggcgcggt gcctggctgg taattctcac tcatgccgcg ccggacgatt
     4741 tatttttctg gcgcggcgac gggaatccat tgaaatcaaa atcaccgatg acagaatcag
     4801 cagcgttccc agccagtccg gtaatgtgaa cgtgatcccc agcaacagca aagagagtag
     4861 cgcgctactt aatggttctg cacagctcaa aatgctggct tttggaccgc caattaattg
     4921 tgctcctttc aggtacagac taaatgtcag ggacgtacca atgaccacca aataaaaaaa
     4981 cgccagaatc aaactgccgt taacgacaaa gtttgttcct tgtctggcat aaaaaggcaa
     5041 cagaatcaga ccgccaatca gcatactcca gccgacgact ggtaacgtgc catagcgggc
     5101 aattagcgtt gagggatagg tggtatagaa tgcagcagca aatgccgagg caatgcccca
     5161 gaacaacgcg gcaggagaga tcgataatga cgtcggatta ccgtgtgtca ccaataaaaa
     5221 agtcccgacc agcgatgtca aaatagcgca gaaaaccaga atgcccgggc gcgatttacg
     5281 caccagtgag aaccaggcga cgataatcgt cggtgagagg aattgcagca ccgttgccgt
     5341 ggctgcgttc gatttttcga tggttagcaa aaaagtgagc tgtacagtta gcgcgccaac
     5401 cacggaaaaa atcagcaggc taatggcatc tttatgattg ttaataatag aaaagatttt
     5461 atcgccatga acaaatgaca gcgtcagtag aatcaaaccg gcgaatatca aacgcgtcat
     5521 agtcaaaaac tgcgacgaca tctggctttg ctccatgatg tattgcgcgc agacccctga
     5581 acttccccac aacacggcgg caatcagaac gttcagcatc ccctttctgg tggaacccat
     5641 ttttccctct gcatactgtt ttgtcgttta tgtatgcagg catgatagca aaaatggcga
     5701 ggatggctct gtttgcgcca cgccgatttt tcttagccca gactggtgag ctaaatatct
     5761 ttaataaaca gcaggcaaat catcgcaata cccaacatcc ctatcgccag ccagaacaac
     5821 gcatggtagc tccagatttc aaccataatt ccgtcaacag agccggcgat aatccagccg
     5881 acgcgtgaag tatttgcata taacgttgtc gcagagccta ttttttcagg catcaagtcc
     5941 tgaaaataaa gcatgccgat accacagaga ataccaagga agatggcatt aagaatttgc
     6001 aattccagct caaccgccgg agtcgtcgcc atgagtacgc tggcgtaaaa acacattcca
     6061 ctcacgatag caatgagcat taatagtcgc ttgccaatac gtttcatgta atagcctgcg
     6121 attaacatca tcggaatttc cagaccggca gcgataccaa tcatttctcc agccagttta
     6181 tcggttagat gcagttcatc aataataaat agcggcatat ttatcatgta gagattattc
     6241 gccgcccaca tcattgaaca gaccacaaaa agtagccgcg tatcccgctt cctgtgggtg
     6301 gagggtaaaa tttctacggg ttgggtaacg acaggaatat ttctttgtat tgatggcaaa
     6361 aacaaccaga ctatcagtcc gcaaacaaca aatgcgatgg cagcggtgag atacatcact
     6421 ttaaaactaa atcccattgc cagttcataa gcgatcggtg gcccgataac ccaggcaagc
     6481 gagatctgag cacgtaaaaa tgtactgaac atgaccgtct cacggcctgt tctgtcggcg
     6541 tgttcacggg cgagggcgaa catttgcggg tttgcggtgg aagcaaaact actcagaagt
     6601 acgcccgttg agaggagaat gaagtagttg cgattccacg caaaaagcgt gcaggccagc
     6661 actccaaata agcaacatag cagaatcagt aatttacggt cgccttgttt atcggagtgc
     6721 cttgccagaa attgactgac cagaattccc ataatagcgc taccggtgaa gaaaaaacct
     6781 accattatag gacgggcttt cagttcatct gcgaggaata tacttagggt aggagtctga
     6841 agagcgcccg caatacccgt cagaaaggcg acaagtaaaa atgccgcggc agtgagatca
     6901 agtatttttg atggagtggt agccgttttt tgcattcttc tgaatcaccg ggcgaaaaaa
     6961 tataactgag ttacaaataa gtaccagtcg aatgagtatt gtattaagaa tttttgagta
     7021 tcgctgatac aaccagaaag caatgctagc gcaatattat agggccagca attgagatta
     7081 ttagtaaaag catgttaaga attaattacc gaatttatat attaa
//
LOCUS       DAZKIV010000195         7027 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZKIV010000195 DAZKIV010000000
VERSION     DAZKIV010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.196"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..797
                     /gene="gadX"
                     /locus_tag="ACHOT6_004288"
     CDS             <1..797
                     /gene="gadX"
                     /locus_tag="ACHOT6_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFP9949398.1"
                     /translation="AYARHKYILTMVNGEYRYFNGGDLVFADASQIRVDKCVENFVLV
                     SRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQNKNLRYAEML
                     RKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWTLARIASELLM
                     SPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGYHSVSYFIYVF
                     RNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1165..1893
                     /gene="gadW"
                     /locus_tag="ACHOT6_004289"
     CDS             1165..1893
                     /gene="gadW"
                     /locus_tag="ACHOT6_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFP9949399.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2256..5369)
                     /gene="mdtF"
                     /locus_tag="ACHOT6_004290"
     CDS             complement(2256..5369)
                     /gene="mdtF"
                     /locus_tag="ACHOT6_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFP9949400.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAMAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5394..6503)
                     /gene="mdtE"
                     /locus_tag="ACHOT6_004291"
     CDS             complement(5394..6503)
                     /gene="mdtE"
                     /locus_tag="ACHOT6_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFP9949401.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6611..6889
                     /locus_tag="ACHOT6_004292"
     CDS             6611..6889
                     /locus_tag="ACHOT6_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949402.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKIVLASPLVITGGNEISICIPRHASHQFQLFMS"
     gene            complement(6890..>7027)
                     /gene="gadE"
                     /locus_tag="ACHOT6_004293"
     CDS             complement(6890..>7027)
                     /gene="gadE"
                     /locus_tag="ACHOT6_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9949403.1"
                     /translation="KNESNITSTLNISQQTLKIQKFNIMYKLKLRRMSDIVTLGITSY
                     F"
BASE COUNT         1777 a         1686 c         1736 g         1828 t
ORIGIN      
        1 ttgcgtacgc aagacataaa tatattctca ccatggttaa tggtgaatat cgctatttta
       61 atggcggtga cctggttttt gcggatgcaa gccaaattcg agtagataag tgtgttgaaa
      121 attttgtatt agtatcaagg gatacgcttt cattatttct gccgatgctc aaggaggagg
      181 cattaaatct tcatgcacat aaaaaaattt cttcattact cgttcatcac tgtagcagag
      241 atattcctgt ttttcaggaa gttgcgcaac tatcgcagaa taagaatctt cgctatgcag
      301 aaatgctacg taaaagagca ttaatctttg cgttgttatc tgtttttctt gaggatgagc
      361 actttatacc gctgcttttg aacgttttgc aaccgaacat gcgaacaaga gtttgtacgg
      421 ttatcaataa taatatcgcc catgagtgga cactagcccg aatcgccagc gagctgttga
      481 tgagtccaag cctgttaaag aaaaaattgc gcgaagaaga gacatcatat tcacagttgc
      541 ttactgagtg tagaatgcaa cgtgctttgc aacttattgt tatacatggc ttttcaatta
      601 agcgagtcgc agtgtcctgt ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa
      661 attattatgg gatgacgccc acagagtatc aggagcgatc ggcgcaggga ttgccgaacc
      721 gtgactcggc ggcaagtatt gttgcgcaag gaaattttta cggcactgac cgttctgcgg
      781 aaggaataag attatagaat tttactcaga cataaaaaaa aaccggcata ggggaccggg
      841 aagaggatag tctgccgtct ccagactaat aaaccgttat aacactccct gttggcacgg
      901 gaaactttgt gctctcagta agttaaatat aacttttact ggaaataaga tcagccattt
      961 ttttataaac ataagctata cgctgtgcga aaatatattc gtgctgcatt tacttattat
     1021 caattaactg ttatgcaaaa ctactttgtg gataaatttt ggtcctacca aatctggcag
     1081 tttttgcgct aagaaacagt ctggcaacat ttcattagta tactgaaatt gaaataatcg
     1141 cagtatgaaa tataagggat aatcatgact catgtctgct cggtgatcct cattcgtcgt
     1201 tcattcgata tttatcatga acagcataaa atatcgctgc ataacgagag tatcgtgctg
     1261 ctggagaaaa atttggcaga cgattttgcg ttttgttcac cggatacgcg acgactggat
     1321 atcgatgagc tgacagtttg ccattactta caaaatattc gtcagctacc acgcaattta
     1381 gggttacata gcaaagaccg tctattaatt aaccagtcac cccccatgcc gctggtgacg
     1441 gcgatttttg atagcttcaa tgaatccggg gtaaattcac cgatactgag caatatgctc
     1501 tacctttcct gtttatcgat gttttctcat aagaaagaac tgatcccctt acttttcaat
     1561 agcatcagca ctgtttcagg aaaagttgaa cgccttatta gctttgatat cgccaaacgt
     1621 tggtatctgc gcgatatcgc ggaaagaatg tataccagcg agagtctcat caaaaaaaag
     1681 ttgcaggatg aaaatacctg tttcagtaaa atattactcg cttccaggat gtcgatggcc
     1741 agacgattac tcgagttacg tcaaattcct ctgcatacta ttgcagaaaa atgtggctat
     1801 agcagtacgt cgtactttat aaacacattt cgacaatatt atggtgtaac gccacatcag
     1861 tttgcgcaac attcgccagg taccttttcc tgacatattt tgcatttgaa tattggtcag
     1921 gatctcacac ctgcttcatg tgaaactctt ccctgatgat ttctgccggg ctaccggcta
     1981 gttctctttc gcagtttgcc aggcggtata ggtattttca ggaggaaaag actcggcatg
     2041 tttgggatta ttaagctgac aattcatacg attacgggaa tgtgggtcag tattgtgctg
     2101 ttcaaattga tgacgaatgg ctggagtggt ttctactttc agtgctgtgt actaagcctg
     2161 gtattcctga cggttagctg gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct
     2221 gaaccttcac gttcaacctt actctcattt acacgttacg cttttttaaa gcgggcaaag
     2281 agatgttcca ctacaacgaa aaagaccgga acgaagtaaa ttgccagcac tgttgcggca
     2341 aacatcccgc ccatcacgcc ggtacctacc gcgttttgcg cgccagaacc ggcaccatga
     2401 ctgataacca gcggcagcac gccgagaata aaggccagag aggtcatcag gattgggcgt
     2461 aaacgcatcc gcgccgcttc gatgattgcc tctatcggcg ttttcccttc tttctgcatc
     2521 atctcaacgg caaattcgac aatcaggatg gcgttcttgg cggaaagccc gatggtggtc
     2581 agcaaaccaa cctggaagta gacgtcatta cttaagccgc gcagatcggt ggccagtaat
     2641 gcgccaacga cgcctaacgg aacaaccaac atcaccgaga acggaattga ccagctctca
     2701 tagagtgcgg cgagggcgag gaacaccacg accagtgaaa tggcatacag tgcaggagcc
     2761 tggtttgagg ataacgcttc ctgatacgac agtccggtcc atgagtagcc gacgcctgcc
     2821 ggaagtttag cgaccaggtc tgccataaat ttcatagcgt caccggtact tttcccggcc
     2881 gccgcttcac ctaaaatctc cattgacggg atgccgttgt agcgttccaa tcgcggtgaa
     2941 ccataggtcc attcggtaga cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg
     3001 cgtacatacc attggttgat gttatccggc aacatacgga acggcgtgcc tgcctggaca
     3061 tacacttttt tcacccgccc ctggttgagg aagtcgttca cgtagctgct gccgaaggcg
     3121 gtggaaattg tctggttgat atcagacagc gccacaccca ttgcctcagc tttcgcggcg
     3181 ttgacgttca ctttgaacat cggcgtatct tccaggccgt tcgggcgtac cccggtgacc
     3241 tggtcaggcg attgcgctgc cagtgataac agctcgtttc gtgcctgggt tagtttttcg
     3301 tgccccaggt taccgttgtc cagcagttcc atatcaaaac ctgacgcggt acccagttca
     3361 gccatcgcgg gtaagttgaa cgggaagacg acggctttat tgatactgct taacgcaatc
     3421 attgcccgct gaatgatcgc ggtaaccgag ttttcctcac cgacacgttc agaccacggc
     3481 ttgagactga taaacgccag gccgttgttt tgcccctggc cgctgaagcc aaagccgcca
     3541 acggtaaaca ccgactgaac attatctttc tcttttgtaa gataataatc cgtcacctgt
     3601 tgcagcactt tcgtggtgtt aaccatggtg gcaccggaag gtaactgcgc ggtggtcata
     3661 aataccccct gatcctcttc tggtaagaaa gaggtcggtg tacgcaggaa cagcaccgcc
     3721 atcccggcgc aaatcagcag gtagaccacc atgtagcgac cggtacaacg caacagcgag
     3781 cgggtgctat cggtatagtg ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac
     3841 agggcgttag gtttgtgacc gccttccggc gcggctttca gaatggtggc gcacagggca
     3901 ggggtcaggc tcattgccac aaatactgaa agcagcatgg aggagatcag cgtgatggag
     3961 aactggcggt agatctcccc ggttgcgccg ctcataaagg ccatcggcat aaacactgcg
     4021 gaaagaacaa cagcaatacc gaccagcgca cgttggatct gccccatcga tttatgcgtc
     4081 gcttccttcg gtggtagctt atcttccgca atgacacgct cgacgttctc caccaccacg
     4141 atggcgtcat ccaccagtaa ccctatcgcc agcaccatcc cgaacatagt taacgtgttg
     4201 atggtgaaac cgaccgccga caagatcgca aacgtcccga gaataaccac cggtacggca
     4261 atcgtcggga tgattgtggc acggaaattc tgcaaaaaca gatacatgac caggaagacg
     4321 aggatgatag cctcaaccag tgttttaaaa acttcctgaa tagaaatttc gataaacggc
     4381 gtggtgtcgt aaggataaac cgtcttcaga cttgcgggga aataggctga taagcggttc
     4441 agctcctctt tgactgcccg cgaggtatcc agggcgtttg ctccggtagc cagtttgatg
     4501 gcgatcccgg cagcaggttt gccgttatag cgcgccacgg tggaataatc ttccgctcca
     4561 agttcgacgc gggcgacatc acgcagcagc acttgcgaac catcttgctg aactttcaac
     4621 aggattttgc caaattcttc cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt
     4681 agttgctggt ctgccgcctg tggcatgcca cccagttgac caccggaaat ctggttgttt
     4741 tgcaccttaa tctgggaaat aacatcggaa ggtaccagat tgtatttatt gagtttttgc
     4801 gggtccagcc agatacgcat ggcgtactcg gaaccaaaga gctgtacgct accaacgccc
     4861 gcggtacggc ttagcgggtc tttgatatta gacgctacat agtccgcgat atcgtactgg
     4921 ttgaggctgc cgttatcaga aataaacgcc gctaccatca gaatattact gctcgactta
     4981 tcaacgctaa tcccctgctg ctgcaccgct tcaggtaatg aaggcatagc gagttgcagt
     5041 ttattttgca cctgaacctg tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg
     5101 atagaggcat tacccgccgc atcactggtt gaagacatgt acatcaggcc atcaagccca
     5161 ttcatatttt gctcaatcac ctgagtgacc gagtcttcta ccgtttgcgc atcggcacct
     5221 ggataggtag cgctgacggt aatggtcggt ggcgcaatct gcggatactg cgcaaccggt
     5281 aagttcatga tcgccagacc acctgcaagc atcataataa tggcaagtac ccaggcaaaa
     5341 accgggcgat caataaaata gttagccatg aaagtcccct taagcctgca acgttattgt
     5401 ttcgattcgg tgctggcgtt ttcctggctg gaggaaattg ctcgtgcttt gatacccgga
     5461 cgaatgcgtt gcaaaccgga aacgatcacc cgatcgccag cctgcaagcc agaggtgaca
     5521 acccactggt cgccgatggc tttgctggct tcaatttcgc gtagctgcac aacatcgtct
     5581 ttatccagaa tgagcgccgt tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc
     5641 accagtaata cattctggcg gctaccttca tccactaatg ccgtgacgta catgccaggc
     5701 agcaagtcac catttgggtt ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca
     5761 tccaccgtcg ggtcggagaa tttcagcgtg ccggtctggc tgtagcgttt accattttcc
     5821 agattgagct gtactggcgt actgccctga acctgtttga tttgcccact ggcgacctct
     5881 tctttcatgc gtaagaaatc ttgcaccgac tgcgtgagat cgacataaat cgggtccaga
     5941 cgttgtacgg taaccagcga atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc
     6001 gacgatttcc cgctgacgcc cgtaatcggc gaggtgacat tcgcgtattg cagattgatc
     6061 gtcgcctgtt caacagccgc tttggcgacg gtgacattgg cttctgcttc attcaactgg
     6121 gtgcgcgcgg tgtcgtaatc ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg
     6181 cggttaaagg tgatgcgggc attgctggcg gtagagagcg ctttcgccag cgagcctttg
     6241 gcggagttta gctcggcctg taaaggtgca ggatcaatct gatacagcga atcgccctgg
     6301 ttcactttat cgccttcgat aaagttgcgt ttaatgataa taccgcccac ctggggacgt
     6361 atctcggcaa cttcataagg aacggttcta ccgggcaatt cgctcaacac attgaccgaa
     6421 ccgggggaga gtgtgacgac accgacctca ggcgtcatgg cggcggcgtt ttccgccgat
     6481 ttgtcatcgc aggcggtgag catcgcgccg cagaataaca acggtattaa cagctttctt
     6541 cttctgttca ttttagtccc tgaaaattct tgagaaataa cggttcttta aattgcgcgc
     6601 gaggagagat atgagggatt tacagaccag cggcatagtg gggttgtcag ccagtaaggt
     6661 gggatacagg cacagtgatc gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct
     6721 gccaacagtt cctgccagca ttcgggcaac gaagggttct actggtggat acacatacca
     6781 ggggaataaa atcgttttag cctctccttt agtaatcacc gggggtaatg aaatcagcat
     6841 ctgcatccct cgtcatgcca gccatcaatt tcagttgttt atgtcctgac taaaaataag
     6901 atgtgatacc cagggtgacg atgtcgctca tacgtcttag tttcagtttg tacataatgt
     6961 tgaatttctg aatctttaat gtttgttgcg aaatattgag cgtcgacgtg atattgcttt
     7021 cattttt
//
LOCUS       DAZKIV010000196         6887 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZKIV010000196 DAZKIV010000000
VERSION     DAZKIV010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.197"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(277..762)
                     /gene="sixA"
                     /locus_tag="ACHOT6_004294"
     CDS             complement(277..762)
                     /gene="sixA"
                     /locus_tag="ACHOT6_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFP9949404.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(965..3109)
                     /gene="fadJ"
                     /locus_tag="ACHOT6_004295"
     CDS             complement(965..3109)
                     /gene="fadJ"
                     /locus_tag="ACHOT6_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFP9949405.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALSRQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3109..4419)
                     /gene="fadI"
                     /locus_tag="ACHOT6_004296"
     CDS             complement(3109..4419)
                     /gene="fadI"
                     /locus_tag="ACHOT6_004296"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFP9949406.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4599..4883)
                     /locus_tag="ACHOT6_004297"
     CDS             complement(4599..4883)
                     /locus_tag="ACHOT6_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFP9949407.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5255..6595
                     /gene="fadL"
                     /locus_tag="ACHOT6_004298"
     CDS             5255..6595
                     /gene="fadL"
                     /locus_tag="ACHOT6_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFP9949408.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1720 a         1842 c         1627 g         1698 t
ORIGIN      
        1 agtttcaatt acatcctgcc tgattattca actctatttt ctctattatc tgtattaata
       61 aatgacaatg agtgtcaaac agacattatt catttcaaca tgtaactttg ggcaaaatca
      121 agatacgcgc agaagaataa tttccgaatg aatgtagaaa gtgttttatt tatagataag
      181 gagagtcttc gatagtagtg aggttgacag atatccgctg cctgatgagc aattatgccc
      241 aatgaatcta cctcattggg cataatttgg aacacatcag atagcttttg ccatcttcag
      301 attacacggg ctcatctgcc agttgaatgt accgtttccg ctttcatcaa gcgttacgct
      361 ggcaatggcc gaggtggtaa acataggcgg cgtttcgccc ggacataact cggcaaccag
      421 atacccgact aacggtaagt gagagatcac cagtaccgaa gcaacacctt cattggtcaa
      481 cgcctgcaaa taggcactga ccagaccaac atcgccgcag ggcgttaact ccggcagaac
      541 ttctgcactg gaaggcaggt tcagacaatc ccctacttct tccagtgttt gctcggctcg
      601 caggaacggg ctcaccagaa cgcgttcgat ttccactttt tgacctttca gccagttcgc
      661 catcaggcga gattcgtcac aaccattagt ggtcagagga cgaaccgaat cactggcggc
      721 atcgagggct gcgtcgccgt gacgcatgat aaaaacttgc atattgcacc gcttttgtta
      781 accagattcg cccattttct ttcatctgaa accagagaaa gaaaacggtg gtcggcattg
      841 tgccttatcc attcaccgaa tgaaacgctg ttttttacct caatggcgta agtatagtca
      901 atatgcgttt acattttgac cagcactccg ccattcagcg cggattcata tagctttgac
      961 cttcttattg caggtcagtt gcagttgttt tccaaaaact ttccccacgt gcgcccatct
     1021 cgaccaaacg ctcgcaaggg gtaaaacggg aaccatactg cgtggccagt cgttgcatta
     1081 ttgcaaccac ttcgcccgcg ccgagagaat cgatatagcg gaacggtcca ccgagaaatg
     1141 gcggaaaacc aatgccaaat accgcaccaa tatccccgtc acgcacgcta cggataacct
     1201 gctcatcaac acaacgtact gcttcattca gcatcaacat cacacaccgt tcagcaacct
     1261 gcggtgcgga gagtcgcccc tgcccttgtg cgccaatgag cgggtaaatg gcgggatcga
     1321 cctgtttttt gcttttacgc cctttctgac cataaagata gaaaccccga ccattttttc
     1381 tgcctttgcg atcgtcgttc aaaattgaag aaacaacatt tgcaggcgcg ctaaaacgtt
     1441 ctccataagc ggcttccagt acaggaataa ttttagtccc ggtgtcgatt cctacctcat
     1501 ccaaaagttg gattggacct accggaaaac caaatttcac tagcgcggca tcaatgtgct
     1561 ctacccgttc accttcggtc agcatgcgga tagcttcatt aatgtaaggc gctaagatgc
     1621 gattgacgta aaacccggct ttgtcacgca cgacaatggg cgttttgccc tgttttttcg
     1681 ccagttttac cgtagtggcg atggtttgcg ctgatgtacc cgcatgagga ataatctcca
     1741 ccagcggcat tttttccacc ggactgaaga aatgcaggcc gataacttgc tcaggtcgcg
     1801 tggcgtgagc ggcgatatca ccaatcggta aagatgacgt attcgaagca aagatggtat
     1861 gagcggcgct attttgctca acttccgcca ccatctgttg tttcaattcg agattttcaa
     1921 acaccgcttc aataatcaga tcgcgatggg caaagccgcg atagtccgtc gttccggaga
     1981 ttaatgccag ctgtttgtca cgttcgctgg ctttgagatg acgacggcga actttgccct
     2041 ccagctgatc ccaactgtac ttcagcgcat gatttatgcc ctgcgggttg atatctttaa
     2101 ttctgaccgg aagccccgct ttacaagcag tgacataagc aataccgccg cccatcaagc
     2161 caccacctaa aattcccacg ctgtttaatg gcgcaggcgg tgcatcacta ccgggatctt
     2221 tcttcacgtc cgtactggca aaaaagatac tacgcaaagc ttgcgattgt ggcgtcatcg
     2281 ccagttcgcc aaacgcccga gcttcggcgt cataaccgct gctggtgccc tgcgctaatc
     2341 ccgtttcaac aacctccagg atgcgttctg tcgccggata attgccttga gttttgtgtt
     2401 ctgttttctt gccgaccatt ttgaacagca gcgcacgacc taacggcccc gccagaatac
     2461 gctcgcgtac aggtagaggg cgggaagatg ggcgatcctt cttcgccagc tcaacagcga
     2521 cttccagcaa aatggagtgc ggaacaacgt catccaccag ccccagcttt aatgcctgtt
     2581 tcgcccgaag ttgttttccg gtgaggatca tctctaatgc tgtgctgacg cctatcagac
     2641 gcggtaaacg ctgggtgccg cctgaaccgg gtaacaatcc aagttgtact tcaggcaaac
     2701 cgagcaccgt tttaggatcg tcagtacaaa cgcgaccgtg gcacgccagc gccaactcca
     2761 gcccaccacc caggcaagcg ccatgaatag ccgcgataac cggaatgggc aaagcatgaa
     2821 tctccgccat caactgttgg ccctgccgcg acagtgcttc cgcttcttgc gccgttttgc
     2881 agttgccaat catgttgatg tctgctccgg cgataaaatt gtccggtttc gcggagataa
     2941 acaccacgcc gcgcaactct ttgttttcac ggagttgctt aataatggcg cgcacctgcg
     3001 aggcaaattc cgccttcagg gtattcattt tctcatccgg tacgtcgatg gtgatgatag
     3061 caatgttgtc cagacgaaca ttaagagtaa acgctgatgc catttccatt attccgcctc
     3121 cagaaccatt gccgcgccaa gcccaccggc agcacaggcg gtaactaaac caaatccacc
     3181 gccgcgacgg cgaagttcat gcaatgtctg ggtaatcatc cgcgcgccgg tcgccgcgaa
     3241 gggatgtccg taagcaatcg aaccgccaag cacgttaaat ttgctatcgt ccacttcgcc
     3301 agtggcatgt gcacgcccca gtacttcacg agcaaaacgt tcactaccca gcaactgaat
     3361 attcgccagt gtctgagctg caaaggcttc gtgcatatcg atcaatgtca gttcgctcat
     3421 cgtcaaaccg gcacgttcca gcgccagcgg tgttgaccag gctggaccga gcaacatgtc
     3481 ctgccagaca tcaatcgcag taaatgcgta gctgcgcaga taccccagcg gcaccagccc
     3541 taattctttc gcccgggatt cagtcatcag gatcaccgct gccgcgccat cggtcagcgg
     3601 cgtactgttt gccgccgtta ccgttccgtg tttgcgatca aacgccgggc gcagctttgc
     3661 gtaatcggca agcgaggaat taccgcgaat attgttgtct tcgacaagcg gttgtttata
     3721 aggagggata aaggcagtca tcacctcttc tttgagtttt ccgtctgacc atgcctgagc
     3781 ggcacgctga tgcgaacggt gcgctaatgc atcttgctgt tctcgggtga tgccgtaggt
     3841 tttcgccatt tgttcggcgg tgtcgcccat ccgcaagcca gtagaatatt ctgctaccgc
     3901 aggcggtacg ggcattaagt cgcgcaaacg caggcgagag aagagtttca ggcgctggct
     3961 catggttcga gctttgttga catcaaccag cacgcgcgcc agttttttac tgacgccaat
     4021 tggcaatacg gaagaggaat ctgccccacc ggcaatcccc gctcggatag ttcccgccat
     4081 caggctttct gcgacgtttg caaccgcctg gaaactggta gcgcaagcgc ggctgacgct
     4141 gtaagcatcg gtatgtacat tcattccggt accgagaaca atttcacgcg caatgttggg
     4201 ggcttcaggc atttgtacga cctgaccaaa gaccagctgt tcaatcactt cagcggggat
     4261 ctcgctgcgt gccagcagtt cgcctaccac catcttccct aaatcaaccg cgggaatgcc
     4321 atgaaaagcc gtcgcctggc gggcaaaagg cgtacgtaaa ccgctaacaa tggcgatacg
     4381 atcgccctgg cgggtaacca gcggtaaaac ctgacccata acactcccct gtaaaaaaat
     4441 aaataaagtg gtctgacctg atcatagtct taaccatttt tttacattta gccaagcgga
     4501 gaaaaaagaa agtgggagct atgacacagt gaagacagga gaaagaaaag aaaacgcccc
     4561 tgccagcaac tgacaggggc gaacgtaccc gagaggaatt aacgcagacc cagctggaag
     4621 atcagcattt cagcttcgca ggcaaaagtg aaatcgatat ccaggcgcac accgtcagac
     4681 tcttcagtga aagtcgggga gattttgcac ggttcggatt ccacgctacg ggctttttca
     4741 gtcagcgccg ccagcgtttg ttctgcttct gcgcggtttg caaacacgcg gctgtaagac
     4801 gcggtgcagt cggagttgtc cataatggtg ccaacatcca tacagcagca aaccggggtt
     4861 tcatcagcac tacatttact catcgttgat ttcctctgta tgtgcaccca aggtgccaga
     4921 taaacgttgt ggatatttta cgcttccgga aagtgctgct ccagttgtta attctgcaaa
     4981 atcggataag tgaccgaaat cacacttaaa aatgatctaa aacaaaattc acccgaatcc
     5041 gcgagtgcgc aacctccaaa ttttgccagc tggatcgcgt ttcttagatc atatttgaaa
     5101 aaagatggaa acatacttgc aacattccag ctggtccgac ctatactctc gccactggtc
     5161 tgatttctaa gatgtacctc agaccctaca cttcgcgctc ctgttacagc acgtaacata
     5221 gtttgtataa aaataaatca ttgaggttat ggtcatgagc cagaaaaccc tgtttacaaa
     5281 gtctgctctc gcagtcgcag tggcacttat ctccacccag gcctggtcgg caggctttca
     5341 gttaaacgaa ttttcttcct ctggcctggg ccgggcttat tcaggggaag gcgcaattgc
     5401 cgatgatgca ggtaacgtta gccgtaaccc cgcattgatt accatgtttg accgcccgac
     5461 attttctgcg ggtgcggttt atattgaccc ggatgtgaat atcagcggaa cgtctccgtc
     5521 tggtcgtagc ctgaaagccg ataacatcgc gcctacggca tgggttccga acatgcactt
     5581 tgttgcaccg attaacgacc aatttggttg gggcgcttct attacctcta actatggtct
     5641 ggcaacagag tttaatgata cttatgcagg cggctctgtc gggggtacaa ctgaccttga
     5701 aaccatgaac ctgaacttaa gcggtgcgta tcgcttaaat aatgcatgga gctttggtct
     5761 tggtttcaac gccgtctacg ctcgcgcgaa aattgaacgt ttcgcaggcg atctggggca
     5821 gttggttgct ggtcagatta tgcaatctcc tgccgggcaa actcctcaag ggcaagcatt
     5881 ggcagctacc gccaacggta tcgacagtaa taccaaaatc gctcatctga acggtaacca
     5941 gtggggcttt ggctggaacg ccggaatcct gtatgaactg gataaaaata accgctatgc
     6001 actgacctac cgttctgaag tgaaaattga cttcaaaggt aactacagca gcgatcttaa
     6061 tcgtgcgttt aataactacg gtttgccaat tcctaccgcg acaggtggcg caacgcaatc
     6121 gggttatctg acgctgaacc tgcctgaaat gtgggaagtg tcaggttata accgtgttga
     6181 tccacagtgg gcgattcact atagcctggc ttacaccagc tggagtcagt tccagcagct
     6241 gaaagcgacc tcaaccagtg gcgacacgct gttccagaaa catgaaggct ttaaagatgc
     6301 ttaccgcatc gcgttgggta ccacttatta ctacgatgat aactggacct tccgtaccgg
     6361 tatcgccttt gatgacagcc cagttccggc tcagaatcgt tctatctcca ttccggacca
     6421 ggaccgtttc tggctgagtg caggtacgac ttacgcattt aataaagatg cttcagtcga
     6481 cgttggtgtt tcttatatgc acggtcagag cgtgaaaatt aacgaaggcc cataccagtt
     6541 cgagtctgaa ggtaaagcct ggctgttcgg tactaacttt aactacgcgt tctgataacg
     6601 cgttcgcctg gataaagtca cctgcatagc aggtgacttt aactccccca cttcaccgaa
     6661 agtagtgtcc tcattgctta cctcctgagt tttgcaaaca gcctgttggc agcttgccct
     6721 ttcaaatcaa taagcggtat ccacagacaa acctgaaggg aaaggcatat tttcaggcgt
     6781 tctgctcgtc cttctcaaag agtttacttt tctgcatttc caggatactc cccccctggc
     6841 tattgtgcgc tcatacactc aaattaaaga taggttctaa ataaatg
//
LOCUS       DAZKIV010000197         6829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZKIV010000197 DAZKIV010000000
VERSION     DAZKIV010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.198"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHOT6_004299"
     CDS             complement(178..1107)
                     /gene="fdhE"
                     /locus_tag="ACHOT6_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFP9949409.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHOT6_004300"
     CDS             complement(1104..1739)
                     /gene="fdoI"
                     /locus_tag="ACHOT6_004300"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFP9949410.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHOT6_004301"
     CDS             complement(1736..2638)
                     /gene="fdoH"
                     /locus_tag="ACHOT6_004301"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFP9949411.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHOT6_004302"
     CDS             complement(2651..5701)
                     /gene="fdnG"
                     /locus_tag="ACHOT6_004302"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5114..5116),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFP9949412.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHOT6_004303"
     CDS             5895..6728
                     /gene="fdhD"
                     /locus_tag="ACHOT6_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFP9949413.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELCRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1494 a         1842 c         1840 g         1653 t
ORIGIN      
        1 atacgcttgt catcagtgac atacccataa ccttatcgat gtgttggagg gaagaacgag
       61 gcaaacagac aattgcggca tgatcttgaa tgggtaacgc ggaatacgag agatagtgtc
      121 gtcacaccct atgagctgta atccgggcga ccagcgccac ccggaaaacg tcagagatta
      181 ctccccttca cccggaaaca ggaacgggtt gatggaactg cgggcatagc cttcttgctc
      241 cattcgcgcg tccagtacca gagaagcgag gtcatctgcc acggcttcaa ctttcggttc
      301 tttttcctga taaagaatct tcaggtaagt gccgcagtca tcgcagcttt cggctttaat
      361 cgcggcctgt tcgtcatcca gcgaccagta atgcagtttg ccgctctgct cgcagttgct
      421 gcattttacg cgcactacgt gccattcggt ttcacacagg ttgcagtgca ggtaacgcag
      481 accctgagtg gtgccaattt gcaccatgct ggacaccggc atagaaccac aaaccgggca
      541 atattgacgt tgctcgccgt attcagcgcg ggctttgccg gggatcagat tggccatctg
      601 cgcccagtag agcgacagtg cagcccagat aaacggcgct ttatcgctgc tgacggatga
      661 gaaatcagag gcaaacagtg cgctggccat atcttccagc tcctgagtcg atgccttctc
      721 cagattctca atcactgcca gcgccgggcc gctcatttca ggtttcagct cagcaatcag
      781 cgccatcagc agcttttgcc agtgcttatc acgcggcaga acgtgaatat ccagcggggg
      841 cttgccctgt gcgctggctt ctttaatgcg cgcggtcaga tccatctcca gcggatggtc
      901 gtacagcacc acttcctggg cgtgggcgat aagcgcagca aagcgcaggt aatcacccag
      961 cggattattt tctgccagct cgcgcagacg ctcggcgcgg cggttgtata aattcttgag
     1021 ccgagggaac aataacggcg gaatcatatc cgccgtacgt ttctcgctcg aacccagctc
     1081 atcttgcggg attatgcgaa tactcattca gctttctttt ccgttgtctt gcggacctca
     1141 cggtaccagc gcgggtgatg tttcttcgcc caggcgctgg taacccatcc ttccaccatg
     1201 gcggtaatcg tgcctttcac ccaaagggcg gcgtagatat gcaccatgat aaccacaatt
     1261 aacgccactg cggcaaatga atgcagcatt aacgcgaatc ggatcaccgg gattgagaaa
     1321 gcaggcgcaa aataaggacg ccagataatc acgccgctca ccagcagcag aaccaggaaa
     1381 ataatcgccg cccagaaaac gcatttctga ccgaagttat aacgcccggt gtcacctact
     1441 tcctcgttga cgacgatctt acgaatattc ttcgcccaaa agatatcatc ccgattgatt
     1501 aggttgtgat gccagtaacg gaaaaacatg atgatgaacg aggcaaacat aaccacgccg
     1561 acaaacgggt gcagaattcg cgccagctgc ggtgtgccca tgatttgcat caaccagttg
     1621 aaggacggga acaaaaagcc cagcccgctc actgccgcca ggatgaagca gaaggcggtg
     1681 atccagtggt tgatacgttc cggcgcggtg tagcgcacga tggtgtcacg tcgtttcatt
     1741 tgcgctcctc gtctttctct tcgtgcagat tattctcttc ctcatccgca cggttcggac
     1801 cgacacccac gtagtggaag atactggctg cgaaggtagc cgcaaagcca acagctgcga
     1861 gcggtttcca gatgcctttc cagaatttca cggtttcgct gatttccggg ttctccggca
     1921 agccatgata cagatttggc ttgtcagcat ggtgcagcac gtacataacg tgtgtaccac
     1981 cgacgcctgc cggatcgtac aggcccgcat tgtcgtaacc gcgggttttc agctccgcca
     2041 cgcgctcgct cgccagcgtt ttcatcgact ctttcgtacc gaagtgaatc gcgcccgttg
     2101 ggcaggtctt cacgcaggct ggttcttgcc caaccaccac gcggtcaacg cacagcgtac
     2161 atttgtagac gcggttgtct tccgggttga ggcgcggaat gtcgaacgga cagcccgcaa
     2221 tgcaataacc gcagccaatg cactgctcgg actggaagtc gacgataccg ttggcatact
     2281 gaataattgc accttccgcc gggcacgctt tcaggcagcc tggatcggaa cagtgcatac
     2341 agccgtcttt gcggatcagc cattccagtt tgtcgttctg ctccacttcc gagaagcgca
     2401 ttaccgtcca cgatttggcg cttaaatcat tggggttgtc gtacacccca atgttattgc
     2461 cgacggtatc gcgaatgtcg ttccactctg aacacgccac ctgacaggct ttacagccga
     2521 tacaggtggt gacgtcgatg agtttcgcca cttcttcctg gaagtcccgc gcctgaggcg
     2581 cgggggtcag accgttagtc gcggaacgac gaatgatatc ttgagattga taagccatat
     2641 gtcgtctccg ttacaccttt tccacattca caaggaagga cttaaactcc ggcgtctgcg
     2701 tgttcgcatc accaacgaat ggcgttaacg tattggcaat aaagcctttt ttagctacgc
     2761 cttcatagcc ccagtgaatc ggaataccga tggtatcgat atctttgccg ttcgctttca
     2821 gcgtgcgaat acgtttggtc accaccgctt tggctttgat atagccacgg ttggaggaga
     2881 ctttcacggt atcgccctgg gcaatgccaa gtttattcgc cagcgactcc ccgatttcca
     2941 caaactgctc tggttgcaaa atcgcgttca acagcgcgtg tttggtccag tagtggaagt
     3001 gctcggtcag acgataggtg gttccgacat acgggaactt atcggcttta cccaatgctt
     3061 cggcgtcgtc tttaaagata cgtgcagccg ggttcgagat aacgtttgga tgcagcgggt
     3121 tagttcccag cggcgtttca aacggctcgt agtgttccgg gaacggacct tccgccatct
     3181 tatcgagggc aaacagacgc cccatgcctt cctgctgcat gataaacggc ccgacgccgc
     3241 tacccggagg cgctgcgctg tagtccggaa tatcccagcc ggtccactta gtgccgtccc
     3301 atttcagcaa ctgacgcttc ggatcccacg ggttaccctg cggatctgcg gaggcgcggt
     3361 tatacagaat gcggcggtta agcggccatg cccatgccca gcccagcgtg ttaccgaggc
     3421 cagacggatc ggcgttatca cgacgcgcca tctggttgcc ttccggcgtc cagctaccgg
     3481 cgaaaatcca gcagccacag gaggttgtac cgtcatcgcg caattgggcg aacgagctaa
     3541 gttgttggcc tttcttgacg ataaccgcac cggttgccgg atcggtaata tcggccagcg
     3601 ccttaccgtt gctctccatc gctacttctt ccgacttcgg ctcatgcgga atggcgtagt
     3661 tccaggtcat gttcagcacc tgatccgggt tcgcgccacc ctgttcggca tacatcttgc
     3721 gcaagcgcag gaagataccg gagaggatct caccgtcagt cagcgcaatc cccggggcgt
     3781 ccgcaccttt ccagtgccac tgcaaccagc gacctgagtt aacgattgaa ccgttctctt
     3841 ctgcgaagca ggtcgatggc agacggaaca cttcggtctg gatcttcgac gagtcaactt
     3901 cgttcagctc accgtggttc tgccagaagt tagaggtttc agtgttcagc gggtcgatgg
     3961 tgacgaggaa cttcagtttc gacagacaac cgatcacttt gtttttgttc gggaatgagg
     4021 caacagggtt aaagccctgg cagatatagc cattgacctt gccttctttc atcatctcga
     4081 agtattgcag gacgtcgtag cctttatccc acttcggcaa ccagtcaaag ccccagctat
     4141 tttccgccgt cgctttatca ccaaagaagg ccttcatcat agagacgaag aatttcgggt
     4201 agttgcccca gtagtttacc tggccttcca gcagcggttt tggcgtgttg gcggtaaggt
     4261 aggtttgcag atcggtctgc ttctcgcttg gcagcgtcat gtaacctggc agactctgcg
     4321 acagtagccc caggtccgtc agcccctgaa tattggagtg accgcgcagg gcgttaacgc
     4381 cgccgccggc catccccata ttgccgagca gcagctggat catcgccatc gtacgaatgt
     4441 tttgcgcacc aacggagtgt tgcgtccagc cgagggcata caggaacgag gcagttttat
     4501 cgtgagcact ggtttctgcg atgtattcgc agactttcag gaacgcgtct tttggcgtac
     4561 cacagatgtt ttcaaccaca tctggcgtgt agcgggaaac gtgctgtttc agcaagttcc
     4621 acacgcagcg cggatgttgc agcgtggtat cgcgtttggc gaagccgttt tcgtccagtt
     4681 cataagtcca ggaggattta tcgtacttgc gtttttccgc gtcgtagccg gtgaacaggc
     4741 catcttcaaa gccgtaatcc tcacgcacta tcaggctggc gttggtatag gcttcggtgt
     4801 attcgcggtt gaatttttca ttgttcagca ggtacagcaa tacgcctgac aggaaagcaa
     4861 tgtcagtacc ggaacgaata ggggcatagt agtcagctac cgccgccgta cgcgtaaagc
     4921 gaggatcgat cacaatcagc ttcgcgccgt tgtgaatttt ggcttccatc gcccagcgga
     4981 acccgaccgg gtgagcttca gcggcgttac cgcccatcac cacgacgagg ttggcgttct
     5041 tgatgtcgac ccagtggttg gtcatcgcac cgcgaccaaa tgttggagca agacttgcta
     5101 ccgttggtcc gtgtcagaca cgcgcctggt tgtcgaccgc gagcataccc agcgcgcggg
     5161 agaatttttg cgttaaatag ccggtttcgt tactcgacgc ggaagcacac agcatcccgg
     5221 tggagagcca gcggttaaca gtcacgcctt cggcgttttg cgcaatgtag ttagcatcgc
     5281 ggtcttcttt catcagtttg gcgatgcgat caaacgcctc ttcccaactg atttgctgcc
     5341 atttatcaga acctggcgca cggtattccg gaaacttcag acggctttcg gagtggatga
     5401 aatccaccag gccagcgcct ttcggacaaa gtgcaccgcg gttgaccggg tgatccggat
     5461 cgccttcgat atggaagata gatgctttgg cgttttttgc tccgtcaccg aggctgtaca
     5521 tcaacagccc acagcctacg gaacaatagg tgcaggtatt acgggtttcg cgggtgcgca
     5581 gcagtttata ctgccgggtt tccgcgagtg ctacgctggg tgcaaaaccc agtgccgctg
     5641 ccgtggtgcc tgccataccg ccagcgcaga tcttaaagaa ctgccttctg ctgacctgca
     5701 tggattgctc cttgtttcga cattgtcacg tcccatttac attcgcttgc tgcatgtgca
     5761 gggagtggga gttatttttc tttgcggaag gggccgcaaa ggtccagaat tggctcaatt
     5821 tccctccatc caggaagggt ttgtaacaga ataccataat gttggtgtgt gtgttcttat
     5881 ctggttaaga gaaagtgaaa aaaacacagc gaaaagaaat cgaaaatgtg acaaatatca
     5941 caggtgttcg tcaaattgag ttatgccggc gtgatgattt acaacaccca cggttagatg
     6001 aggtcgcgga agaagttccc gttgcgctgg tctacaacgg catttcgcat gtggtgatga
     6061 tggcgtcgcc caaagacctt gagtactttg cgctcggttt ttcgctttcc gaggggatta
     6121 tcgaaagtcc gcgcgatatc ttcggcatgg atgtcgttcc ttcctgtaat ggtcttgaag
     6181 tacaaattga gctttccagc cgccgcttta tggggttgaa ggagcgccgc cgggcgctgg
     6241 cgggacgtac gggctgtggc gtatgcggcg tggagcaact taatgacatc ggaaaaccgg
     6301 tgcagccgct accgttcacc cagacgtttg atctcaacaa actggatgat gcattacgtc
     6361 atctcaacga ttttcagcca gtggggcaac tgactggttg tactcacgcc gctgcctgga
     6421 tgttgccatc tggcgaactg gtcggcgggc atgaagacgt gggtcgccat gtggcgctgg
     6481 acaaactgtt aggccgccgg tcacaagaag gggaaagctg gcagcaaggt gcggtactgg
     6541 tttccagtcg tgccagttat gaaatggtgc aaaagtcggc gatgtgcggc gtagagattt
     6601 tgtttgcggt gtctgccgcg accacgcttg ctgtagaagt ggctgagcgc tgtaatctga
     6661 cactggtagg tttttgtaaa ccgggtcggg caacggttta tacccatccg cagcgtttga
     6721 gcaattaatt aaaataagtc ctgcgatata tattaaattg caggacttat tcatttcgtg
     6781 aattttatta ttttatttat aaataaaacg cgtgtagtgg cgttttctt
//
LOCUS       DAZKIV010000198         6750 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZKIV010000198 DAZKIV010000000
VERSION     DAZKIV010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.199"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..201)
                     /gene="dinJ"
                     /locus_tag="ACHOT6_004304"
     CDS             complement(<1..201)
                     /gene="dinJ"
                     /locus_tag="ACHOT6_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFP9949414.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEA"
     gene            387..1160
                     /gene="yafL"
                     /locus_tag="ACHOT6_004305"
     CDS             387..1160
                     /gene="yafL"
                     /locus_tag="ACHOT6_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9949415.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
     gene            complement(1217..1573)
                     /locus_tag="ACHOT6_004306"
     CDS             complement(1217..1573)
                     /locus_tag="ACHOT6_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFP9949416.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREFSAQM"
     gene            complement(1566..1844)
                     /locus_tag="ACHOT6_004307"
     CDS             complement(1566..1844)
                     /locus_tag="ACHOT6_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFP9949417.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            complement(1949..3661)
                     /locus_tag="ACHOT6_004308"
                     /pseudo
     CDS             complement(1949..3661)
                     /locus_tag="ACHOT6_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3633..4418
                     /gene="lafU"
                     /locus_tag="ACHOT6_004309"
     CDS             3633..4418
                     /gene="lafU"
                     /locus_tag="ACHOT6_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFP9949418.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            4489..5544
                     /gene="dinB"
                     /locus_tag="ACHOT6_004310"
     CDS             4489..5544
                     /gene="dinB"
                     /locus_tag="ACHOT6_004310"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFP9949419.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            5541..5993
                     /gene="yafP"
                     /locus_tag="ACHOT6_004311"
     CDS             5541..5993
                     /gene="yafP"
                     /locus_tag="ACHOT6_004311"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFP9949420.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            6238..>6750
                     /locus_tag="ACHOT6_004312"
     CDS             6238..>6750
                     /locus_tag="ACHOT6_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFP9949421.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGS"
BASE COUNT         1634 a         1738 c         1721 g         1657 t
ORIGIN      
        1 agcttcgctg tttttgattg attgaatggt taattgatta ggctcgcgta aatcaaacgg
       61 caatgccttt tcacgcgcga cctttgtgag ggttatgcga accaggtcag agatggtcag
      121 ccccatcccg gccagtacgt ccgccgcctg attcttcaga tcttcatcga ttcgggcgcg
      181 aacaaatgcg ttagcagcca ttggggtatc tcctgtgctg tatatttatt cagcttgaat
      241 tgtagctcaa atgagcaaca gcgacaatcc atgagtaatt taataaacgg atgtttttac
      301 ataattaact gaaatatatt tattaatttt ataataatgg tttattaagt ttgttcaggt
      361 acgggccggg atacactgca acatcaatgc atgatcagcc attattgagt atgtccttgc
      421 cgtcgattcc ctcttttgtg ttgtcgggat tactgttgct ttgcttgccg ttttcttcat
      481 ttgccagcgc caccacatca catatcactt tcagctacgc cgcccgccag cggatgcaaa
      541 atcgtgcgcg tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctatattg
      601 tggaaggcaa tgccgaaagc agaagggcgc tacgccagca caaccgcgaa cagataaaac
      661 agcatccaga atggtttcct gcgccgctca aggcgagtga cagacgctgg caggcgctgg
      721 cggaaaacaa ccatttctta agcagcgacc atctgcataa catcaccgaa gtggcgattc
      781 accgtctgga gcaacagctt ggcaagcctt acgtctgggg cggtacgcgg cccgatcaag
      841 gcttcgactg tagcgggtta gttttttatg cctacaacaa gatccttgag gcgaaactcc
      901 cacgcacggc taatgagatg tatcactatc gccgggcaac cattgtggcg aataacgacc
      961 tgcgccgggg agatttgcta tttttccata tccacagccg cgagattgcc gaccatatgg
     1021 gggtgtatct gggcgacggt cagtttattg agtcaccgcg caccggggaa accattcggg
     1081 taagtcggtt agctgaacct ttctggcagg atcacttttt aggcgcgcgg cggattttga
     1141 cggaggagac gattttgtaa atgggtaata ttgacgaact ggcaaaaggt cggatgccac
     1201 gcggcatccg acctgtttac atctgggctg aaaactcacg ttctaagaca tcaacaatga
     1261 gctggttttt tgaaatggaa tgttgtctgg cgacgatggt cagccgcgaa tcaatgtgat
     1321 gaggaacacg aagcgtcagg cgtttttgct cttcttcttc acgaaaaggg gcaataccgt
     1381 cctcttcaca atccgccatc cactcatcca gcgcgctttc gccttcacga tataatgcct
     1441 caatacttgt ggctacaaag tcacaataac cgtttacatt gaggaacttg ccgcgaaatg
     1501 cgttgatatc ggcttcaaat gtcactgcgg ctggttgacc tttaattgtg ataatgttcg
     1561 atgctttatt cataatctac ccctacacag ttaacaaacc actccttaag acctgctact
     1621 gcgcctttat ccatcacatt ctttggatgt ggctcatgtg tgctgaattt ggtcgttcca
     1681 atctgaaatt ttcgtctgga gccgttgtta ttttttattg ttccacccac agctttaatg
     1741 agtgcaacaa catcatccca tttaattcca gattttggcg gctccgtcat tacatcgtcg
     1801 aatgtttttc tatgacgaga acctagcgcg ctgaccttaa tcatactatc aatccatgac
     1861 gtcattgtat ggtgtcatcg tagtctcttt gcagtgtgac gtcaattaat ggtgtcaggt
     1921 gctgcaaagc atggaaaaga atcccccatc aactcaacgc ccccataatc ttcaactcca
     1981 gctcatccgg cacttcgtta tacgacagca catgcagccc cggcgcaaac aaccttgcat
     2041 aacgcgccag caaagggcgc agctgcggtg gcaccagcag caccgggtct ttccccgccg
     2101 ctttcatctg ctctttcacc tgtggcatcg tactctggaa ctggttgagc atattcgggt
     2161 ccactggcac gctgtcgagc atcactttac cgccctgttg cgcctgattc actaggttag
     2221 tcagcagatt ttccagctca ttattcaggg tatacaccgt cagctcctgc ttgcgaacga
     2281 acggatgggt aatgctgcgc cgcagcgcca ggcgcacatc ggctgccagc agaatatgat
     2341 ctttggtcac cgcgctactg gcgaccagca cggtggcgat ggtgacgata tcgcgcaggg
     2401 aaacgccttc ggtcagcagc gcccggtaca ctttcagcaa ctggctgtaa ttgagcaccg
     2461 cgcttaaatc ctccgccagg cgcggtgcca tcgacgccaa acggttatgc aactgcgtaa
     2521 tatcgtcata gttaaataaa tcaggaatat agctgcgcac aatcttattt acatgggtag
     2581 caatcacgct ggcgctgtcg atcacctgat accccatatt cagcgccttc gctttctgcg
     2641 caggctggct ccaggtcacc ggcatcccgt acgccgggtc gttccccagc acgccgtcta
     2701 tctcgccgta ggtttcgctg gagggcagcg ccatcagttt atccgccgga atatccgctt
     2761 catcagcctt aatgccgttg atgaaaatag cgtactggct gggcttaagg cggaagtttt
     2821 cccgaatgcg gatctccggc agcagcacgc cgttgccgtc ggatatcacc tgtcgcacgc
     2881 cgcgaatacg ctgggtgagc gggttgccct gggctttgtc caccagcgcc accagcttgt
     2941 agccgaggct taagctgatg ggctcgatca gcggaatggt ttcccaactg acctgttgct
     3001 cgctggtttc ggtgatggtg cgggtcagcg tttcgagact tttctcttcc gcttccgccg
     3061 cctgcgggcg tttgctcatc cgccagccgg taaagccaag cagggcgctg aacagcaaaa
     3121 acggcagatg tggcattcca ggcactaccg ccagtacgaa cataattccg gtagcggtgt
     3181 agagcaccga tgggcttgcc agcaactggt tacgcacgtc atgggcgata tcgccgttgt
     3241 cgctgacgcg ggtgacgata atcgccgccg cggtggagag tagcagggaa gggatttgcg
     3301 ccaccagccc gtcgccgatg gtcatcagca catactgctg gaaggcggcg tcggcgctca
     3361 ggttgtattt gaagatcccg atacagacgc cgccgatcag gttgatcgcc agaatcatca
     3421 tcccggcgat agcatccccg cgcacaaatt tcgatgcccc gtccatcgcg ccgtagaaat
     3481 cggcctcgct ggcaacatct ttacgtcggg tttgtgcctg cgcctgattg atcaacccgg
     3541 cattaagatc ggcgtcaatc gccatctgct taccgggcat cgcgtccagg gtaaagcggg
     3601 cagaaacctc ggagatacgc tcggcccctt tggtgacgac aataaagttg attgtcaaca
     3661 gcgtcagtaa atccgagcgt gaaagcatta tcgccgcgct gcacgggcag tcgattttta
     3721 acggtggtgg gctgtcgccg ttaaataaaa tcagcccgtc tcatccgcca aaacctgcca
     3781 ccgtggcggt gccggaagag acggagaaaa aagcgcgcga cgtcaacgag aaaacagcgc
     3841 tgctgaagaa gaaatccgcc accgagcttg gcgagctggc aaccagtatc aacaccattg
     3901 cccgtgatgc gcatatggaa gcgaatctgg agatggagat tgtcccgcag ggattacgcg
     3961 tgctgattaa agacgaccag aatcgcaata tgttcgaacg cggcagcgcg cagattatgc
     4021 cgttctttaa aacgctgctg gtggagctcg cgccagtgtt cgactcgctc gataataaaa
     4081 ttattattac cgggcatacc gatgcgatgg cctacaaaaa caatatctac aacaactgga
     4141 acctttcggg cgatcgcgcg ctttcagctc gtcgggtgct ggaagaggcc ggaatgccgg
     4201 aagataaagt gatgcaggta agcgcaatgg cggaccagat gctgctggac gccaaaaatc
     4261 cgcaaagcgc gggcaaccgg cgcattgaga ttatggtgct gaccaaaagt gcgtccgata
     4321 cgctgtatca atactttggt cagcatgggg ataaagtggt gcagccgctg gtgcaaaagc
     4381 tggataagca gcaggtgctt tcgcagcgaa tgcgctaaat gctgaatctt tacgcatttc
     4441 tcaaaccctg aaatcactgt atactttacc agtgttgaga ggtgagcaat gcgtaaaatc
     4501 attcatgtgg atatggactg ctttttcgcc gcagtggaga tgcgcgacaa tcccgccctg
     4561 cgcgatatcc ctattgctat tggcggcagc cgcgaacgtc ggggggtgat cagcaccgcc
     4621 aattatcccg cgcgtaaatt tggcgtacgt agcgcaatgc cgacagggat ggcgctcaaa
     4681 ttatgcccac atctcacctt gcttccgggg cgctttgacg cctacaaaga agcctcaaat
     4741 catatccgtg aaatcttctc gcgctacacc tcgcgcattg agccgttgtc actggatgag
     4801 gcttatctcg atgtcaccga tagcgtccat tgccacggtt ctgcgaccct catcgcccag
     4861 gaaatccgcc agacgatttt cagcgagctg caactgacgg cgtctgcggg cgtggcacca
     4921 gtaaagtttc tcgccaaaat cgcctccgac atgaataaac ccaacggcca gtttgtgatt
     4981 acgccggcag aagttccggc atttttacaa accttaccac tggcaaaaat ccccggcgtc
     5041 ggcaaagtct cggcggcaaa actggaagcg atggggctgc ggacctgcgg tgatgtacaa
     5101 aagtgtgatc tggtgatact gcttaaacgc tttggcaaat ttggccgcat tttgtgggag
     5161 cgtagtcagg ggattgacga gcgcgacgtt aacagcgaac ggttgcgaaa atccgtcggc
     5221 gtggaacgca cgatggcgga agatatccac cactggtctg aatgtgaagc gattatcgag
     5281 cggctgtatc cggaacttga acgccgtctg gcaaaggtaa aacctgattt actgattgcc
     5341 cgccaggggg tgaaattaaa gttcgacgat tttcagcaaa ccactcagga gcacgtctgg
     5401 ccgcggctga ataaagctga cttaatcgcc accgcgcgta aaacctggga tgaacgccgc
     5461 ggcgggcgcg gtgtgcgact ggtggggctg catgtgacgt tgcttgaccc gcaaatggaa
     5521 agacaactgg tgctgggatt atgaataaca tacaaataag agactatcag cctggcgatt
     5581 ttcagcaact atgcgctatt ttcattagag cggttacgat gactgccagt cagcattatt
     5641 caccacaaca aattgccgcc tgggcgcaga ttgacgaatc tcgctggaag gagaaactcg
     5701 cgaaatcaca agtgcgggtt gcagtcatta atgcaaaact ggtcggcttt attacctgcg
     5761 ttgaacacta tatcgatatg ttatttgttg accctgaata cacccgccgt ggggttgcca
     5821 gcgctttgtt aaaacctttg attaagtctg aatccgaact tacggtggac gcaagcataa
     5881 ccgcaaaacc cttttttgaa cgttatggtt ttcagacagt taagcagcaa cgcgttgaat
     5941 gtcggggaga gtggtttatt aatttttata tgcgttataa acagcaacat taaatccagc
     6001 ttgcaatgaa aataacgcaa gcctagtatg tgcgcgtttt cttattccac aaactgcaag
     6061 gaggtaaatc atgacaaatc ctttatcaat gactctttgc agacctttcc aggattaatt
     6121 cttttttctt gccctggatt cgtctgccat ttcctgattt tttaattgtt actctcaata
     6181 attcaagtcg caacacatgc aacttgaagt atgacgagtc tatttacgga atggtatatg
     6241 ggcaattata ttcgtccctt atccgatgcg gtatttacca tcgcatctga tgacctgtgg
     6301 atcgagagtt tagcgatcca acaattacac accacggcaa atttacccaa catgcagcgc
     6361 gtagtgggga tgccagattt acatcccgga cgcggctatc cgattggcgc agcgttcttc
     6421 tctgttggtc gtttttatcc ggcactggtc ggcaatgata tcggctgcgg tatggcgcta
     6481 tggcaaacag atattctcgc tcgcaaatac aacgccgata agtttgaaaa gcgattatca
     6541 gatctggatg acgttgctga agaaagctgg ctggaggaaa acctgccgtc agcatttgca
     6601 cagcatccgt ggcgcagctc gcttggctct atcggtggcg gcaatcattt cgctgaactg
     6661 caacaagttg atcaaatcat caacgctgaa ctgtttgcac tggcaggtct ggatgcacaa
     6721 catctgcaac tgttggttca tagcggctcg
//
LOCUS       DAZKIV010000199         6743 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZKIV010000199 DAZKIV010000000
VERSION     DAZKIV010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.200"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            219..782
                     /locus_tag="ACHOT6_004313"
     CDS             219..782
                     /locus_tag="ACHOT6_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949422.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            864..3506
                     /gene="yfcU"
                     /locus_tag="ACHOT6_004314"
                     /pseudo
     CDS             864..3506
                     /gene="yfcU"
                     /locus_tag="ACHOT6_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            3526..4278
                     /locus_tag="ACHOT6_004315"
     CDS             3526..4278
                     /locus_tag="ACHOT6_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9949423.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4253..4801
                     /locus_tag="ACHOT6_004316"
     CDS             4253..4801
                     /locus_tag="ACHOT6_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949424.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4798..5268
                     /locus_tag="ACHOT6_004317"
     CDS             4798..5268
                     /locus_tag="ACHOT6_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949425.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5265..5789
                     /locus_tag="ACHOT6_004318"
     CDS             5265..5789
                     /locus_tag="ACHOT6_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949426.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5776..6648
                     /locus_tag="ACHOT6_004319"
     CDS             5776..6648
                     /locus_tag="ACHOT6_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFP9949427.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
BASE COUNT         1783 a         1512 c         1699 g         1749 t
ORIGIN      
        1 attaattaaa tctacattta ctctttagta aaattaaatt gcatcagggg tgtggaaaca
       61 tcactatgcg gccccacacc aacgaataca attttctacg acctgttctt tttggatcgc
      121 atgccgtatt caatggctgg gtataaacat tggctattga ctgaatcaat gtattgcaat
      181 gtttatattt ttaattattt atagtttaag gacaaataat gagtaagttt gttaaaacag
      241 ctattgctgc ggcaatggtg atgggcgtgt tcacctctac ggcaacaatc gctgcgggta
      301 acaacggtac tgcacgtttt tacggcacca ttgaagactc agtgtgttct atcgttccgg
      361 acgatcacaa actggaagtt gatatgggtg atatcggtgc cgaaaaactg aaaaataacg
      421 gcaccaccac gccgaaaagt ttccagattc gtctgcaaga ttgcgtattt gatactcagg
      481 aaacaatgac cactaccttt actggtaccg tttcttctgc aaatagcggc aattattaca
      541 ccattttcaa taccgatacc ggtgcggcat ttaacaatgt tagcctggcg atcggtgact
      601 ctctgggtac ctcttacaaa agcggcatgg gtattgacca gaaaatagtg aaagacactt
      661 ctaccaataa aggcaaagcg aagcagacac tgaactttaa agcctggctg gtcggcgcag
      721 ctgatgcgcc agatctgggt aatttcgaag caaataccac cttccagatt acttacctgt
      781 aaggcatacc cccatagcga cactgctatg ggggatttaa atggattttg accgaacaag
      841 aataaaaacg gatagaaacg tgtatgcctg accattctct ttttcgatta cggattcttc
      901 cgtggtgcat tgcgctggca atgtcaggga gttatagcag tgtctgggct gaagacgaca
      961 ttcagtttga ttcccgtttt ctggaattaa aaggcgacac aaaaattgat ctgaagcgtt
     1021 tttccagcca gggatatgtt gagcccggaa aatacaattt acaggttcaa ctaaataaac
     1081 agccattggc ggaagagtac gatatttact ggtatgctgg tgaagatgac gcgagcaaaa
     1141 gctatgcttg tctgacaccg gaactggtag cgcagtttgg tttaaaagaa gacgtggcga
     1201 aaaatctgca atggagccac gatgctaaat gcctgaaatc cggtcaactg gaaggcatgg
     1261 aaattaaggc tgatttaagc cagtccgcat tagtcatttc actaccgcag gcttacctcg
     1321 aatatacctg gcccgactgg gatccgcctt cacgttggga tgacggcatt tccgggatcg
     1381 tcgcggacta cagcatcaac gcacaaaccc ggcacgaaga aaatggcggt gatgatagta
     1441 acgagatcag cggcaacggg acggtcgggg ttaacctggg gccgtggcgt atgcgtgctg
     1501 actggcagac taactatcaa catactcgca gtaatgatga tgatgaattc agcggcgatg
     1561 aaactcaaaa aaaatgggag tggagtcgct actatgcctg gcgggcgtta ccatcattaa
     1621 aagccaaact ggcgctgggc gaggattacc tcagatccga tatttttgat ggttttaact
     1681 atgttggtgg cagtgtcagt actgacgatc aaatgttgcc tcccaatctg cgcggctacg
     1741 cgccagacat ttccggcgtg gcacacacca cagcaaaagt gaccgtcagc cagatggggc
     1801 gtgtgattta cgaaacgcag gttccggctg gaccgtttcg tattcaggat cttggtgatt
     1861 ctgtctccgg tacgttgcat attcgcattg aagaacagaa cggccagttg caggaatatg
     1921 acatcagcac cgcctcgatg ccatacctca ctcgtccagg tcaggttcgc tataagatca
     1981 tgatgggccg tccgcaagag tggggacacc atgtcgaggg tgaatttttt tctggtgctg
     2041 aagcttcctg ggggatcgct aacggctggt cgttatatgg cggcgcacta ggagatgaaa
     2101 actatcagtc tgcggcgctt ggcgtcggtc gcgatttgtc tacattcggc gcggtcgcgt
     2161 ttgatgttac tcactcgcac accaaactgg ataaagacag cgcttatggc aaaggttcgc
     2221 tggacggtaa ctccttccgt gtgagttatt ccaaagactt tgaccagctc aacagccgcg
     2281 ttacttttgc tggatatcgc ttctcggaag agaactttat gaccatgagc gagtatctgg
     2341 atgccagtga cagcggaatg gtacgcacgg gcaacgacaa agagatgtac accgccactt
     2401 ataaccagaa cttccgcgat gcgggtgttt cggtttatct caactatacc cgccatacct
     2461 actgggatcg cgaggagtag acaaactaca acatcatgct ctcgcactat ttcaatatgg
     2521 gcagtattcg taatgtcagc atctcgatga ctggctaccg ttacgagtat gacaaccagg
     2581 ccgacaaagg catgtacatt tcgctcagta tgccgtgggg cgacaacagt accgttagct
     2641 ataacggtaa ctatggcagt gggacggaca gcagtcaggt cggttatttc agccgtgtcg
     2701 atgacgcgac tcactatcag ttgaacgtcg gcaccagtga caaacacacc agcgttgacg
     2761 gctattacag ccatgatggt tcgctggcgc aggttgacct cagtgcgaac taccatgaag
     2821 ggcaatacac ctctgcgggc ttgtcgttac agggcggcgc aacgcttact gcccacggtg
     2881 gcgcacttca ccgtacccag aatatgggtg ggacacgctt gttgattgat gccgatggcg
     2941 ttgccgatgt tccggtagaa ggtaacggtg ctgctgttta taccaatatg tttggtaaag
     3001 ccgtcgtttc tgacgtcaat aactattacc gcaatcaggc gtatatcgac ctcaacaaac
     3061 tgccggaaaa cgccgaagca acccagtcgg tggtacaagc cacgctaact gaaggggcga
     3121 ttggctaccg caaatttgcc gtcatcagtg gtcaaaaagc gatggcggtg ctgcgtttac
     3181 aagacggcag ccatcctccg tttggcgcag aagtaaaaaa tgataacgag cagacagtgg
     3241 gccttgtcga tgatgacggc aatgtttatc tggctggggt gaaacctggc gaacacatga
     3301 gtgtgttctg gagtggtgtt gcgcattgcg atatcaacct gccggacccg ctgcctgccg
     3361 atctgtttaa cggcttgtta ctgccatgcc agcataaagg caacgtcgcg ccggttgttc
     3421 ctgatgacat aaagcctgtc attcaggagc agacgcaaca ggtgacaccc accaatcccc
     3481 ctgtttctgt ttcagctaac caataacgtg attaaggaat gatccatgtc tgatttactt
     3541 tgctctgcca aattgggtgc tacgacgcta gcgttactgc ttagcgctgc cagtttgtct
     3601 gcccaggctt ctgtaacgcc agatcgtacc cgtttgatat ttaatgaaag cgacaaatca
     3661 atcagcgtga cgttgcgcaa taacgatccg aaactgcctt atctggccca aagctggatc
     3721 gaagatgaaa agggtaacaa gatcagttca ccgttaactg ttctgccacc ggtgcaacgc
     3781 atcgattcaa tgatgaacgg gcaggtaaaa gtgcagggaa tgccggatat caacaaattg
     3841 cctgccgatc gtgaaagcct gttctacttc aacgtgcgtg agatcccgcc aaaatcaaac
     3901 aaagccaaca cgctgcaaat agcactacaa acgcgtatta agttgttctg gcgaccgaag
     3961 gcgctggaga acgtcagcat gaaaaacccc tggcaatata aggttacttt aacccgcaac
     4021 ggtcaggaat ttaccgtcaa taacccgaca ccgtattacg tcattttcag taatgccagc
     4081 acacagaaaa atggcaatcc ggcagccggt ttctcgccaa tagtgatgtc gccgaaaacc
     4141 tctgcaccgc tgaatgtgaa gatgggtagc gtgccggtac tgacctatgt caacgactat
     4201 ggcgctcgga tgccgctctt tttcacttgc aacggtaaca gttgccaggt cgatgaagag
     4261 cagagccgca aaggctgagg ccgggagagc cagtatgaaa ggatcttgtt ctgtctgggc
     4321 ggtaactggg ttactgatat gttgtgttgg tagcggcttt gcagttgcag cgagtaagtc
     4381 agtaagtatg aatttacggg tgcttgtcga tgcccctcct ccttgtacgg taaatggtgc
     4441 ggcagttgag tttggtaatg tattcattaa taagatcaat ggggttgatt ataaacgtcc
     4501 cattgactac tcgcttgttt gtaacaatct ggcaatggat gacctgcgtc tacagatgca
     4561 ggcgacaacg gtcgtgatta acggtgaaac agttatcagc acggggattc ctgggtttgg
     4621 tattcgggtt cagaaaagta gtgatcacac aatactcgac ttaacctctg gctcatggtt
     4681 gccatttaat ttttcctcgg gagttcccgt tctggaagct gtgccggtga aacagagtgg
     4741 tacaacgtta gcggcagctg aatttaacgc cagtgccaca attgtggtgg attaccaatg
     4801 aaacggatct ctcttatttt gctgtggggc ttttgctcga tggcactctc aaacgtctct
     4861 tttcacggtt atttagtgca gccacctaac tgcacgatta gcaacgctca aaccattgaa
     4921 ataacttttc aggatgtgct tattgatgac attaatggca gcaattatga gcaaaccgtg
     4981 ccgtattcca ttacctgcga tacggcggtt cgcgacccat taatggaaat gaccttgagc
     5041 tggagtggca caccaagcga ttttgataat gctgcagtaa gcagcaatat cactggttta
     5101 ggcattcagc tcaaacaggc agggcagagt tttacgatca atacgccact tgtggtgaat
     5161 gaaaccgatt taccggtgtt aacggctgtg ccagtaaaaa agagtggtgt cgttctgcca
     5221 gaggcggact ttgaggcctg ggccacgtta caggtagatt accaatgaag aaaaagcgta
     5281 cgttgttttt tattagtagc ctgatgttgc tgggcagtgg aaccacaata gcaggagata
     5341 atttgcactt taccggcaat ttaatcagta aatcgtgtac gccagtgatt aatggtagcc
     5401 agttggcaga agtacatttt cctgccattg ccgccagcga tttaatgaac cttgggcagt
     5461 cagagcgagt gccattggtg tttcaattga aagattgtca tagttctaca ttgtttaatg
     5521 tcaaagtaac gttgacagga acagaagata gtgctctacc cggctttctg gcctttgact
     5581 cctcgtcatc agcatccggc gctgggattg gtattgaaac tgcggcaggc acatcggtgc
     5641 caatcaacaa tacaacaggc gtcacgctcc cgctgaatca gggaaataac agcctaaatt
     5701 ttaatacctg gttacaggca aagagcgggc gagatgtaac gtccggagac tttagtgcca
     5761 cagtgacggc aacctttgag tatttctgaa atgaaaaaat ggaccataat tttcgcgagc
     5821 ctaatgctgc tcgttctgag tgttgtcggc gcatctaaga gttatgctgg agataaactt
     5881 atgagtgcga gttttgacag tgtgagagtt tattatgcca tggacaaggt aaccggtgct
     5941 gtagcgagca gtgttttcaa tgttacggtg ataacgccaa aggaggttgc atatgggaaa
     6001 tatgattctt ttgcgtataa aggcgatacg ttgcgggtta tttcctggtc tgggtcgggt
     6061 tcagctccaa ctttggtact gactgacttt gatacaatta ataactcaaa ttgccctggc
     6121 atagatacga aaatttttcg ttgtgcatat atgacgttta aaattaccgt tgcatctgat
     6181 gattatggtt gcccttggat cgcatccttt tattcctata cagatttacc cggatttgga
     6241 agctatacgg cccctacagt gcataacaca atatgcccaa cgataccggt cgcttcatac
     6301 gatatctcct ggagtgaaaa ttatgtcagc cataataagg cgttacgaat tcagtccacg
     6361 gggtcaacag tgaccaccac gctttcgacc tacttgatgg aaggcggcag actctgtgat
     6421 ggcagtaatt tttctgataa tgatggcaga ggagcatact gccgcgcggt atcagaattg
     6481 ttaaccttta cctcttacgg ttgtgataaa tcaacggtaa cggttacgcc aactcgtcat
     6541 ccggtaacgg ataaagtact acacgatatt gtggtgaatg tgaatactag tagtgggcaa
     6601 ccgattgatt cgacgtgtcg ttttcagtat gtattgaatg aactgtagtt cgtagtattg
     6661 ctctgatgcg tcttatcagg ccttgtatta tccctccagt gcagagaaaa tcggccagtt
     6721 ttctctgcct gcagtccgca tgc
//
LOCUS       DAZKIV010000200         6596 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZKIV010000200 DAZKIV010000000
VERSION     DAZKIV010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.201"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..212)
                     /locus_tag="ACHOT6_004320"
                     /pseudo
     CDS             complement(24..212)
                     /locus_tag="ACHOT6_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(265..1263)
                     /gene="lpxM"
                     /locus_tag="ACHOT6_004321"
                     /gene_synonym="msbB"
     CDS             complement(265..1263)
                     /gene="lpxM"
                     /locus_tag="ACHOT6_004321"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFP9949428.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSARQRALINLSLCFPEYSDKEKE
                     NIVDAMFTTASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
     gene            complement(1337..3058)
                     /gene="cptA"
                     /locus_tag="ACHOT6_004322"
     CDS             complement(1337..3058)
                     /gene="cptA"
                     /locus_tag="ACHOT6_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFP9949429.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETTRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            complement(3152..4258)
                     /locus_tag="ACHOT6_004323"
     CDS             complement(3152..4258)
                     /locus_tag="ACHOT6_004323"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFP9949430.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIEYRFDINRTAQIIVSLASQAKGKCNR"
     gene            complement(4258..5079)
                     /locus_tag="ACHOT6_004324"
     CDS             complement(4258..5079)
                     /locus_tag="ACHOT6_004324"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFP9949431.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            complement(5346..5588)
                     /locus_tag="ACHOT6_004325"
     CDS             complement(5346..5588)
                     /locus_tag="ACHOT6_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949432.1"
                     /translation="MNGYLTSMAGLLQVLLLFWGGTGVVNIYDARDSHDGPVHQCMRR
                     DIVRVFFRGSGIRMQKIPLLDWFYAINQDDNLLIFY"
     gene            complement(5681..5815)
                     /locus_tag="ACHOT6_004326"
     CDS             complement(5681..5815)
                     /locus_tag="ACHOT6_004326"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949433.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
BASE COUNT         1845 a         1618 c         1415 g         1718 t
ORIGIN      
        1 ccgctcagta aatatcaata cagttctgac gagccgctta caagctgtga ttcatacaga
       61 ctgagcacac gggctgcgac agcaacaccg atgcgttcag caagcttcag ggcttcactg
      121 cgaaattcag gcgaatgcta tttacggggt tttttactgg ttggtactgt ttttttgtca
      181 tatgagccac ctctgactga gagtttactc acttagcgtg tgtccactat tgctgggtaa
      241 gctcattatc ctgaaaaacc tgcctcagta aggatcctct tccccttctt tacgcgtttt
      301 cagcaacttg aggatccacg tatactgctc aggatgtgga cgaacgaaat tttcaaccac
      361 ttcgtttatc tgtcgcgcaa tatgagcatc actcttctgt cgtatagcaa gtggcgggct
      421 gacatgaata gtcagatgat gggtctttcc gtcataaaca gggaaaagcg gtattatccg
      481 tgcaccactg atcctgctga gccgtccgat aaccggtaaa gttgctttat acgtcgcaaa
      541 aaaatctgca aactcactga attccgggcc atgatcctga tcaggaagat aatatcccca
      601 gtatccctgc cggactgagc ggacaaagga ggcaataccg tcattacgtg cgtgcagttt
      661 tcctccgaaa cgacgacgaa cactgttcca gacataatcc accaccggat ttctctggtt
      721 atgaaacatc gctgccattt ttctgcccga cgcagccatc agcattgcgg gaatatccac
      781 tccccaggca tgtggcacaa gaaaaatgac cttttcattg ttctgtgcca tcttctccac
      841 aatctccaga ccattccagc gtatacgatg cgaaatttta tcaggcccgg acagtgctaa
      901 ttcagccata agcacaacgg ccatggaggc tgttgtaaac atggcatcaa cgatattctc
      961 cttttcttta tcactgtact ccggaaaaca aagcgataaa ttaatcaagg cccgttgacg
     1021 tgcgcttttc cccagacgcc caaccagcat gcccaatttt cccaacaatg gatctctcag
     1081 tgcggggggg accattgcaa acacacagat aataccgata cctgtccacg tcatccagta
     1141 acgggggaag cataatgtaa ggttaaagcg gggaataaac tcagttctga tatttttcat
     1201 atttaccggt atctgcctgc ttatctcatt atgataacag agtacagcag aaggtggtac
     1261 catccctgga ggccaccgga cagcagagga aaacacatcc tcctgccttc agaacggaac
     1321 cgaagataag aaaatactat ggctcaccat acggaagagt gtcgaaatca gtgagcccat
     1381 tctttttata cggatttcca atccagcggg tggtttccct gaattgcgga ttcaccagag
     1441 aacgggtcgg atcgtaacca tcgtatgtca gtcccgccaa atctgaccag gtgtggatca
     1501 gttcagccag actgtatttg cggtcaacat actgcgaaaa atctcgcgga tgtgcagcat
     1561 gccacttttc cgaggtccac agcaggaacg gaacagtgta catgggacgt gtggggttat
     1621 cttcattccg tccctgcgtt ttatatggcg gagtatcata tacttcttca ccatgatctg
     1681 aaaaataaag cagaaagccg tccggcgcag ttgctctgaa gtcctttatc agactggcca
     1741 ccacatgatc gttaaacaga ttggcattat cgtaatcgtt atacacttcc agctctttcg
     1801 catttaatcc agtgggaata tgccctgtaa tgccatcaaa ccgcccctga ccttccggat
     1861 aacggtattt atacttaatg tgcgtaccca gcagatgcac gatgatcagt ttctttggtg
     1921 cagggtcatt cagcacttcc cggaacggct tcagcacgtt agtgtcatat tcacgcgcac
     1981 tttgtgttcg ctgctgattc atgtagtact gcctgtccgt ctggcgcgaa aatacagtga
     2041 gcatggtgtt acgggctgtg attgtctgct ggttggtaat ccagaatgtt ttatagcctg
     2101 cctgcttcat catgttcatc agcgacggct gcgtcagata cagatcagga ttcttttcgt
     2161 tggcgaaagt aagggcctgt tgcaatgctt caatggtgta cggacgcgat gccaccacat
     2221 tattaaacac agtaagaccc ggatcggttt tacgcagtgc atccagctcc ggcgtcgttt
     2281 cacgtagata cccgtataag ctcatgcgtt cgcgctgtgt cgactcacca atcaccagga
     2341 ccagtgtgcg tggtctctcc cctgattcat ccctgagatt acccagtggt ggcagtgcgc
     2401 tattttcatt caggaaggtt gtcagtgcat tcagttgctg atggtactga taataactgg
     2461 atacaaactg ccagggggca gcaggctcca ttcgggatgc cagtttgcct agagtatcat
     2521 tcagcggctc ctgtctgatt aacgatttca gaacaaccgg atgcagaagc agagcataaa
     2581 gcagcaggaa agagacaatt ctccgccatg gcaaaggaat atatacagga cgcagacgtg
     2641 tccacagaaa aacggacacc gcagtatata ccagcgagat aagcaacagt ttaaggctga
     2701 aatactggct gaaatattca ccagcctctc tggcattcgt ttcaaacata acgaaaagaa
     2761 cactttgaga gaattcatga ccatagagaa aataataaca aagtgctgcc agcgacgtgc
     2821 cccagagaat gaaaccgaca acagctgcaa ttatttttat ccgatcagga tagagaaaca
     2881 ccgggatcaa ccacagacaa ctgaataaca gtgagtcccg tattccattc gttccgctgt
     2941 acccactgct gaagatgatg acctgcagga gagtggaaaa aaagccaaaa taaaaaattg
     3001 cccatcccag cgcactccag ctgaaagtag gcctgttctg tccggtattt aaatgcattg
     3061 accgtccccg tatttaaaca atgtgataaa ttactccgtt acttaccgga aaaccgctga
     3121 acaaaattcg ggctgaaaag aggatccgcc gttatctgtt acatttcccc ttagcctgac
     3181 tagccagaga cacaatgatc tgtgccgttc tgttaatatc aaaccggtac tcaatatctt
     3241 ctctggcgct ggctgccatc atccggaagc gttccggtcg ggataaaaaa tcgcgcagtg
     3301 cgccggtcca tgcagacaca tcccccacgg gtaacagcgt ccctgtcaca ttcttctgaa
     3361 tgacatcagg gatcccgccc gtctcactgg cgataacggg cacgccagag actgacgctt
     3421 cagccagtac cataccaaac gcttcatttt ccgaaggcat gaccaccaca ctggcaatcc
     3481 ggtagaccgg taacgctggg aaaagggcac ctgccattaa cacatctccg ctcattccca
     3541 ggtgttctgt ctgctgacgc agacgtgctt cgtattcttc acgcccggcg cccaccacga
     3601 gccagcgaaa tgatttccct tccatcttca gctgatacaa tacacgcagc ataaattcat
     3661 gtcctttttc gggacgtagc atccccacct gaacgataag cggaacattg tctgctgatg
     3721 cagcccaggc gtggatatgc aggggtaacg gtcgcatggc ttcattatgc aatgcgggcc
     3781 agtcgaaacc cggtggaata accgttaccg gtgtcctgac accttccgcc atcagatgcg
     3841 ccatcatggc tgagctaggc acaacaatga aatcacacag ataattcagg gaaaacgttc
     3901 gggtcttacg ggtgatgtag gttttttgtc tgacaatact gaagcggtga cagcatatca
     3961 gacggctcag tcctgctata ttactgtcat ggccactatg gcagatgacc agatcaggtt
     4021 taaattcccc gataatccgt cgaagtctga ggatggaagg aaggtgaagg ctgttcctga
     4081 aaggaataaa agtgacatca tgccctcttt ttctggcttc cggagcaatt ttactttttt
     4141 ccctgcaggc aagtaaaacg gaatgtccct gcttttgaag agcagtcatc tgggccagtg
     4201 cctgcagctc ctggccccca atatctgacg aagattcagt gaataaaatt ttcatcatta
     4261 attatctggt aatcttggcc ccttatgaag agccagtact gaagaaaaaa gtggcgttgt
     4321 ataataaaaa aggcgtcgtt tcagccagcc ggaatgttcc cgctctttgg tgctgatacg
     4381 ccctatgcgt aatgaaccat tttcaggaca gttcattctt ctttcggttg tatataaata
     4441 agagaaacca agcttaccgg cgagattaat ataatcccga ttataatatc cttcaggcca
     4501 gcacagatgg gaactacaga accccagctt ttccgtcaga cattgttttc ccaccagaat
     4561 atcttctttc atgagccggc actgttcagc acgtgataca gacaacctgt cccagcgttt
     4621 atgtgaatgg gtgtgcacat gaaattcaac cagtccggaa tcacgcattt cccggacttc
     4681 agaccagcga agcatgacct catctgaacg gttatcggca atcaaccgtt cacagtcgcg
     4741 gtgagaatat tcctgctcct gcctgctacg tacgtttccc ttaccaatca gccccgtaat
     4801 gagaaaaata tgtgcatgaa ggttatattc cttcaggacc ggccatgctc tgagccagtt
     4861 atcaagataa ccatcatcga atgtgagcat gacactcttt cgtggaagcg ttcccccctg
     4921 ataaaaatat tcaagctcag cagatgttac cgtccgccag ttattatctg cgagccactt
     4981 catctgttca caaaatgttt caggtgagag tgtcacaagc cccggacaac gactgacatg
     5041 atgatacatc agtacgggaa gatgccttgc attcaacata aaaaattatc gtctgttaag
     5101 cattctgaaa atatcctcgc gtaaacaaca gtgaggatcc atagatatca cacatggtga
     5161 tattattgtg taatcccgga atggtccgga gattaccgac aacagggatt ttttaatatt
     5221 tttatcagtt aatcaaccag aacttaaatt tcccttaacg catatgctct ttttaatcag
     5281 attttctgtt tttcagaaaa aacagaatac cgcaatttca gtaaacacag cgagagatat
     5341 ccgcctcagt aaaaaatcag aagattatca tcctgattta ttgcataaaa ccagtcgagt
     5401 agcggaattt tctgcatccg gataccactc cccctgaaaa atactcgcac aatatcgcgc
     5461 cgcatgcact gatggaccgg gccatcgtga gaatctcttg catcgtagat attaaccaca
     5521 ccagtgcccc cccagaacaa cagtaatacc tgtagcagac ctgccataga ggttaaataa
     5581 ccattcatat ccctcttcta cccattcatc ccgaagtttc agccagcgac gaatggactg
     5641 tacctcctgt ttcagccgag gggctgagtt gacaaaccgt ttagagccgc cggatattca
     5701 ggttaccggc ctgcaaatca atatcagaca gttttatacc taataactct gatgcttgga
     5761 caccatgaag ataccccata aggagcaggc agtgattacg ttccgggtgt ggcactttga
     5821 agacagcact gtgcaactta tgaatctcac ccgggttcag atatttttgt tgtccattac
     5881 ctgtccttat ttattgaaag tcgatattag tttaaaaagc tgctaatcat gacaccatta
     5941 cagaagtaaa attaatttat tttaaataca taaaattatt gttcatttat tttttttgcg
     6001 aacattcata aactaaaaaa aaacaatgga taaaaccaat aacattgcat aataatacac
     6061 ctcccttata aataatggag aagaaaatga aaaggcggta tatcacctat gctgaacctg
     6121 tttaggatgc tgtgtaaatg ccttttctcc gaagtgaccg tccaagcggt caccgaattc
     6181 gataataaag cggctcattg ccatacacca gtccttcaac ggcatcgtcc atttctgagg
     6241 cgcagactgg atagctaaag tgtagcggta ttaggagctg ttatttagtt cgaatatcga
     6301 aaatttttgt gtacgttggt cgtatcaatc tctttttatt tgaattagtt tgataacaga
     6361 tttttgcaat ctgataactc atttatttat tgagggataa atgcaatatt gtacaatatg
     6421 tttccgccat tagcaaaaga atccacaagg cgcttgcact aatgcatggt aatgcctcca
     6481 attagttgaa ttaggggtat atgaaccgcc ccgggaatcc tggagactaa actccctgag
     6541 aaagaggtaa acaggatgac taaaaataca cgtttttccc ccgaggtccg tcaacg
//
LOCUS       DAZKIV010000201         6350 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZKIV010000201 DAZKIV010000000
VERSION     DAZKIV010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6350
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.203"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(271..1941)
                     /gene="betA"
                     /locus_tag="ACHOT6_004327"
     CDS             complement(271..1941)
                     /gene="betA"
                     /locus_tag="ACHOT6_004327"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFP9949434.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRANTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(1955..3427)
                     /gene="betB"
                     /locus_tag="ACHOT6_004328"
     CDS             complement(1955..3427)
                     /gene="betB"
                     /locus_tag="ACHOT6_004328"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFP9949435.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3441..4028)
                     /gene="betI"
                     /locus_tag="ACHOT6_004329"
     CDS             complement(3441..4028)
                     /gene="betI"
                     /locus_tag="ACHOT6_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFP9949436.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4157..6190
                     /gene="betT"
                     /locus_tag="ACHOT6_004330"
     CDS             4157..6190
                     /gene="betT"
                     /locus_tag="ACHOT6_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFP9949437.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHHNFIHLHREAPGHSVMFPDA"
BASE COUNT         1312 a         1743 c         1739 g         1556 t
ORIGIN      
        1 tcttcagata aaataaaatc tcaatcagat cgatatcctg tttgatttgt tcacgcataa
       61 tatatccaga aaataaaatc tgtcgcagat aaggttgtat taatagtctg tatcaggaat
      121 gttcgggtta aatatcagca aaaagccagc atcatgaata ctggatatga agcatgagag
      181 ttacctcagt gtttatataa ggattcggtc cccctctctg gaacggtaac tctcaatctg
      241 atcggttcct gcgttagttc acatcacgac tcattttttc gctctcaccg gcatcccatt
      301 tgccacaaaa tatcccgccg tgctcctcgg cagcgcttcc tgtccacgaa tcatatccgc
      361 tattttctcg ccaatcataa ttgtcgtggc gttcaaattc ccggtgataa tctgcggcat
      421 aatcgacgca tccaccacac gcaggccttc taacccgtgt acgcggcctt cgccgtcaac
      481 cacggacatc tcgtcgtaac ccattttgca ggtaccgcac ggatggaagg cggtttcggc
      541 gtggttacgc acgaactcat cgagctgttc atccgtctga cattccgtgc cggggctgat
      601 ttcgcggcca cgatactgat ccagcgcggg ttgatgcatg atctcgcggg tgatgcgaat
      661 tgcgtcgcgg aactcctgcc agtcctgctc gtgcgacatg tagttaaaca gaatcgccgg
      721 atgctggtgc gggtcgcggg atttaatccg cacatgccca cggcttggcg agcgcattga
      781 gccgacgtgg cactggaaac cgtgctcctt caccgcattc gagccgttat agttaatcgc
      841 tactggcagg aaatggtact gaatattcgg ccacgcaaat tcctcacggc tgcgaataaa
      901 tccacctgct tcaaagtggt tgctggcacc aacgccagtg ccgccaaaca gccactccgc
      961 accgattttc ggctggttcc accactgcag ggcagggtag agggaaaccg gttctttgca
     1021 ctcatattgt agatacatct ccagatgatc ctgaagattt tcgccgacgc cgggtaattc
     1081 atgcaccagc ggaatatcaa actccgccag cagttcagcg ttgccgacgc cggagcgttg
     1141 caggatctgc ggtgaggcaa tcgcgcctgc acataacagc acttctttgt tggccgttgc
     1201 gcgggttggg atggtgctgt cgccttccag ccattcgacg cccaccgcgc gtttgccgtc
     1261 aaaaatgatg tgatcggtca tagcgtgagt acgaatggtc aggttagggc gcgatttggc
     1321 ctgatcgaga taaccgcgcg cggtattggc gcgacggccc tgcggcgtga cggtgcgatc
     1381 catcggacca aaaccttcct gctgataacc gttgagatcg tccgtgcgcg ggtagcccgc
     1441 ctgcacgccc gcttcaatca tcgcttcaaa cagcggattg acgccgggtt tggaggtagt
     1501 gacgctcacc gggccatcgc caccgtggta gtcgttctcg cccacatcgc gagtctcggc
     1561 cttgcggtag tagggcaggc agtcgagata actccagttc tccagaccgg gttcttgcgc
     1621 ccagttatcg agatccagcg cattgccacg gatgtagcac atgccgttga tcagcgacga
     1681 tccacccagg cctttaccgc gtccgcactc catgcggcgg ttattcataa acggttcagg
     1741 ctctgtttca taggcccagt tgtagcgttt gccctgtagc gggaacgcca gcgctgcggg
     1801 catctgggtg cggaagtcaa agcgatagtc cgggccgccc gcttcaagca gcagcacgga
     1861 ggtattcgga tcttcagtca gacgggtagc gagaacgttg ccggctgagc cggcaccaat
     1921 aatgatgtag tcaaattgca aataaacctc ctggttagaa tatggactgg aatttagcca
     1981 tctcaacctg gatggacttc acctgggtgt aactctggag cgtcatcacg ccgttctcgc
     2041 gaccaatgcc ggagtgtttg tagccgccaa cgggcatctc tgccggggat tcgccccagg
     2101 tgttgatcca gcaaataccc gcttccagct gatgaatgac gcgatgcgcg cggttcaggt
     2161 ccgccgtcac gatacccgcc gccaggccgt agtcggtatc attggcgcgg cgaatcactt
     2221 cgtcctcaga ttcataagtg agaagcgaca tcaccggacc gaagatctct tcgcgcacga
     2281 tggtcatttc gtcgcggcaa tcggtgaaca cggtcggtgc aacccatgcg ccgttatcga
     2341 agccatcgcc tttcagtaca tcgccgccgc acagtacgcg cgcgccttcc tctttgcctt
     2401 tggcgatata gcgcagcacg ttatcgcgat gcgggaagct gaccagcggg ccgaagttag
     2461 tttgcggatc gaaaacgtcg cccgcgcgaa tgcgctcaac gcgcgccaga attttctgct
     2521 caaatgcggc tttgcatttc gccggaacga agacgcgggt gccattggta cacacctgac
     2581 cggagctgaa gaagtttgcc atcatggcga tatcggcggc gagatcgaga tccgcatcat
     2641 cgaaaacgat cagcggtgat ttaccgccca gttccatggt cacttctttc agggaagagg
     2701 ccgccgagtt agccatcact tttttgccgc tggcgacacc gccggtaaat gacactttgg
     2761 caatgcccgg atgctcggtc agatattgcc cggtctccgc gcccaccccc ggcaacacgt
     2821 taaatacgcc gtccggcagg cccgcttcgc tgtaaatttc agccagcttt aacgcggtaa
     2881 gcggggtgac ttcgctcggt ttgaaaatca ttgcgttgcc tgccgccagc gccggggcgg
     2941 atttccacag ggcaatctga atcgggtagt tccatgcacc aatccccgcc accacaccca
     3001 gcggttcgcg gcgggtataa acaaatgacg tctcacgcaa cgggatctgg ctgccttcca
     3061 gcgccgggat cagcccggcg tagtactcca gcacgtccgc accggtaacg atatcgacgg
     3121 ttgaggtttc cgaatatgct tttccggtat cgagggtttc cagttttgcg agttcgtcat
     3181 tgcgttcacg cagaatatca acggcccgac gcagaatacg cgagcgctcc atagcggtca
     3241 tcgccgccca gattttttgc ccctgttggg cactcttcac ggcgcgatcg acatcctcac
     3301 gcccggcggc ctgcacggtc gccagcacgt taccgttggc cgggttaatg gtctcgaagg
     3361 tgcgaccgct ggtggcggag gtataaccac catgtatata aagctgctgt tctgccattc
     3421 gggacatcac gtctcctcgg ttaatcggtg ggtaaatgct gagtaataaa gtgacgggtc
     3481 agcgagtgtg cgagaggttt atccagcggt ttgccgctta gtgccgcgcg cagccataat
     3541 ccgtcaatca gcgcggcaag gccgtagccc gcttcctgtg cctgttcgcg cggcaattcg
     3601 cggcgaaact cgctcaccag attcgacagc aagcggcggc tgctgacctg ctgtaaacga
     3661 tagagcatcg gctgatgcat actgctggcc cagaacgcca gccaggcttt catcgccgca
     3721 ctgctcacct gagtttcatc gaagtttccg gcaacaatcg cctgtaatcg ctgctctgca
     3781 ctgccctgcg gaagtgcatg taatcgattc aaaaccgcgt cacgtaactg actggtgatg
     3841 tcgcgcatgg ttgcttccag cagaccattt ttgtccctga aatagtggct gatgatcccc
     3901 gtagaaacgc ctgcacggcg ggcgatctgc gcgatcgttg catcgtgcat gcccacttca
     3961 tttattgctt ccagtgtggc gtcgatcagt tgtctgcgcc ggatcgactg catccccaat
     4021 ttgggcattt tcgccactcc attcatcagc gttgtttatc tattaaagcg gttattgatt
     4081 ggacgttcaa tataaaatgt gtcttaattg ttacgaattt gattttaaat ggtaacaata
     4141 acagtgggga tactggatga cagacctttc acacagcagg gaaaaggaca aaatcaatcc
     4201 ggtggtgttt tacacctccg ccggactgat tttgttgttt tccctgacaa cgatcctgtt
     4261 tcgcgacttc tcggccctgt ggattggccg cacgctggac tgggtgtcta aaaccttcgg
     4321 ttggtactat ctgctggcgg caacgctcta tattgtcttt gtggtctgta tcgcttgttc
     4381 gcgttttggt tcggtgaagc tcgggccaga acaatccaaa ccggaattca gcctgctgag
     4441 ttgggcggcg atgctgtttg ctgccgggat cggtatcgac ctgatgttct tctccgtagc
     4501 cgaaccggta acgcagtata tgcagccgcc ggaaggcgcg ggacagacga ttgaggccgc
     4561 gcgtcaggcg atggtctgga cgctgtttca ctacggctta accggctggt cgatgtatgc
     4621 gctgatgggc atggcgctcg gatactttag ctatcgttat aatttgccgc tcaccatccg
     4681 ctcggcgctg tacccgatct tcggtaaacg gattaacgga cctataggtc actcagtgga
     4741 tattgcagcg gtgatcggca ccatcttcgg tattgccact acgctcggta tcggtgtggt
     4801 gcagcttaac tatggcttga gcgtactgtt tgatattcct gattcaatgg cggcgaaagc
     4861 ggcactgatc gccttgtcgg tgatcatcgc aacgatctct gtcacctccg gtgtcgataa
     4921 gggcattcgt gtgttatcgg aactcaacgt tgcgctggcg ctggggctga tcctgttcgt
     4981 gctgtttatg ggcgacacct cgttcctgct taatgcgctg gtgctgaacg ttggcgacta
     5041 tgtgaatcgc tttatgggaa tgacgctcaa cagttttgcc ttcgaccgtc cggttgagtg
     5101 gatgaataac tggacgctct tcttctgggc atggtgggtg gcatggtcgc cgtttgtcgg
     5161 cctgttcctg gcgcgtatct cgcgtgggcg taccattcgc cagttcgtgc tgggcacgtt
     5221 gattattccg tttaccttca cgctgttatg gctctcggta ttcggcaata gcgcgctgta
     5281 tgaaatcatc cacggcggcg cggcttttgc cgaggaagcg atggtccatc cggagcgcgg
     5341 cttctacagc ctgctggcgc agtatccggc gtttaccttt agcgcctccg tcgccaccat
     5401 tactggcctg ctgttttatg tgacctcggc ggactccggc gcgctggtgc tggggaattt
     5461 cacctcgcag cttaaagata tcaacagcga cgcccccggc tggctgcgcg tcttctggtc
     5521 ggtggcgatt ggtctgctga cgcttggcat gctgatgacc aacgggatat ccgcgctgca
     5581 aaacaccacg gtaatcatgg ggctgccgtt cagctttgtg atcttcttcg tgatggcggg
     5641 gttgtataaa tctctgaagg tagaagatta ccgccgtgaa agtgccaacc gtgataccgc
     5701 gccgcgaccg ctggggcttc aggatcgcct gagctggaaa aaacgtctct cgcgcctgat
     5761 gaattatccg ggcacgcgtt acactaaaca gatgatggag acggtctgtt acccggcaat
     5821 ggaagaagtg gcgcaggagc tgcggttgcg cggcgcgtac gtggagctaa aaagcctgcc
     5881 gccggaagag ggacaacagt tggggcatct ggatttgttg gtgcatatgg gcgaagagca
     5941 aaactttgtc tatcagattt ggccgcagca atattcggtg ccgggcttta cctaccgcgc
     6001 acgtagcggt aaatcgacct actaccggct ggaaactttc ctgttagaag gcagccaggg
     6061 caacgacctg atggactaca gcaaagagca ggtgatcacc gatattctcg atcagtacga
     6121 gcggcaccat aactttatcc atctccatcg tgaagcgccg ggccatagcg tgatgttccc
     6181 ggacgcgtga ttgttattgc ataaaaccgc gccatgtctg catatggcgc gggaatcatt
     6241 gactcgctgt aaaaaattat gcatatatta tatatgattg tcatgtcata tcctgcaatt
     6301 cctcttgtat cacttcatga ttttatattt tattaattaa cgattttatt
//
LOCUS       DAZKIV010000202         6342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZKIV010000202 DAZKIV010000000
VERSION     DAZKIV010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.204"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..98
                     /gene="ymgI"
                     /locus_tag="ACHOT6_004331"
     CDS             <1..98
                     /gene="ymgI"
                     /locus_tag="ACHOT6_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFP9949438.1"
                     /translation="NYVNNSERSLVKQILIEDEIIVCATYLIPDI"
     gene            100..444
                     /gene="ymgG"
                     /locus_tag="ACHOT6_004332"
     CDS             100..444
                     /gene="ymgG"
                     /locus_tag="ACHOT6_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFP9949439.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            448..783
                     /gene="ymgD"
                     /locus_tag="ACHOT6_004333"
     CDS             448..783
                     /gene="ymgD"
                     /locus_tag="ACHOT6_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFP9949440.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRELKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(840..3161)
                     /locus_tag="ACHOT6_004334"
     CDS             complement(840..3161)
                     /locus_tag="ACHOT6_004334"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFP9949441.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGSHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(3278..3487)
                     /locus_tag="ACHOT6_004335"
     CDS             complement(3278..3487)
                     /locus_tag="ACHOT6_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949442.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(3887..4105)
                     /gene="ymgF"
                     /locus_tag="ACHOT6_004336"
     CDS             complement(3887..4105)
                     /gene="ymgF"
                     /locus_tag="ACHOT6_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFP9949443.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(4237..5760)
                     /gene="pdeG"
                     /locus_tag="ACHOT6_004337"
     CDS             complement(4237..5760)
                     /gene="pdeG"
                     /locus_tag="ACHOT6_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFP9949444.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETQEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(6092..6340)
                     /gene="ymgC"
                     /locus_tag="ACHOT6_004338"
     CDS             complement(6092..6340)
                     /gene="ymgC"
                     /locus_tag="ACHOT6_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFP9949445.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT         1786 a         1444 c         1360 g         1752 t
ORIGIN      
        1 tgaattatgt gaataatagc gagcgttctt tagttaagca gatcttaatt gaagacgaaa
       61 ttattgtgtg tgcaacttac ttaataccgg acatctaagt tgaagaaaaa aatacttgcc
      121 ttcgggctta tctcagcatt gttctgctcc acgccagcga tggcggatat gaatcgcacc
      181 acgaaaggtg cattgctagg cgcaggcgtt ggtttactta ctggcaatgg tgttaatggc
      241 gtactgaaag gtgcagctgt tggcgctggt gttggtgcag taacagaaaa aggccgcgac
      301 ggtaaaaatg cgcgtaaagg tgcaaaagtg ggtgccgccg ttggtgctgt cactggcgtt
      361 ctgaccggca atggcctcga aggggcgatt aaaggtgccg tgattggcgg tacgggtggt
      421 gcaattctgg gtaaaatgaa ataaggaatg atgatgaaaa aatttgcttt actggcgggc
      481 ctgtttgttt tcgcaccaat gacctgggcg caggactaca atattaaaaa cggtttgccg
      541 tcagaaacct atattacttg cgcagaagcc aacgaaatgg caaaaactga cagcgcacag
      601 gttgcagaaa ttgttgcggt aatgggtaat gccagcgttg ccagccgtga attaaaaatt
      661 gagcaatctc cggaactgag cgccaaagtg gtagaaaaac tgaatcaggt ttgcgctaaa
      721 gacccgcaaa tgctgttgat cacggcaatt gatgacacca tgcgtgctat cggcaaaaaa
      781 taatatttca tatgtaccgc gtcgatgaat acgacgcggt tttttagcta acggcgggat
      841 cagaacttat atttcaggcc caccatcact gcggtgtcat tgtagccatt atcacccagc
      901 tgcacgccca cattccccca gacgctggcc gctggattca ggttgccatt tacgccaagt
      961 ttaatttccc ccaaattact cgccccatct tgttcgactt tcacaccgtt cattgagaca
     1021 gcaaaatctt tactgttatg gatccagttc atttcaacaa aaggttcaaa gtcgtcaccg
     1081 tgcgaaccgc tgtttttctc ctgagtacga ataaaggtgc ggaaaccaag tcgggtctgg
     1141 atgttgttat ttgcgtcatt ctcaatacgt gagccattgg cttcggtgtg cgcattatga
     1201 tcgacgccca tccagatagc ctgtgcctgt ggctggaata tccacgtgta tttcgccgct
     1261 tccagaccaa aggatttatt cagggcaaag ccgtaacccg cttccagcga gacggttgcg
     1321 cctttcgcac tataggattc ataggctaat tcatcccctt tcaccgagtt tttaaaccag
     1381 ctatattgcg cccaggcgtc aatgtatgcg ccttttttac tgatgtcatc ggcaaaccag
     1441 gtggcataca gccctgcgca atagcctctg acactccctt tcgaacgata atccgacacg
     1501 ctggaatgag ttgagttgta gtcgcgggca taaccagcca tcactcccaa acgccaactg
     1561 tcactgtcgg taaaaccacc ggttaacaga tcggccccta actgcgagac gtagcgatgc
     1621 gaggtcgttc tcaactgtcc gttgctgtca cgccaggcat tatgcccgcc aatttgacgt
     1681 agccaaagtc ggcttgaacg ctcctgttca gttacaggat cgatgtagcg catttcaccc
     1741 gcacggtcgt tcagacgcat aacaaaaaga gtgttagcgg ctgccaggtt ggcgacgtaa
     1801 cttccagctt cgaggcgcag gacgggggta ggcgcaacag taggcgctga acttcctccg
     1861 gagtttgttt caccgccgct cgttgcctgg aagttagtca gataccagtt tttattggtc
     1921 gcttcggtat tacctcgggt taatgaatat tcgtaggcac cggcgaaaat cctcgaatcc
     1981 ttaataaatt ccccatttga ttccccctca acgctgataa tctctatccc atttaacgtg
     2041 taagctccgc tgcctcctgc attggtaact ttaactcggg ttgttccaga ggtatttcca
     2101 ttaataacga ctttatccgt agctgaatta tcatcaccca attcagttct gagatgcaat
     2161 acgccattat taccaacgta attttcagtt attgttaatc ttgttggttc gacatcccgt
     2221 ccatctgccc gggaaatata gaccgtacta ttatcaaccg tgaggttgct caccgttgag
     2281 tcagctttaa tatcccaggt actattatct gaaagcgaca gataggtatg agtgttatca
     2341 tctgttgaaa tattagctga acccgttatt tttgaattag ttgcatcgac ggtcaaatga
     2401 cctttaatat tattgataaa aacatctgca gccgatgata cctcactatc cgtaatggtc
     2461 acgttactcg tctcattttt atggtagagg atttcatttg ctgaccgtag tgagctacca
     2521 gataaaatat ctaccgttgc ttcctgacca tttatggaag actccagcac aattccagac
     2581 ccctgatgcg cgactgcaga tgtatttttc agcgtcaccg tgccgccatt ataaatttta
     2641 gcaatcgcgg cgtcaggacc cgttgtataa atttctccct ggttaaatga aactgtcccc
     2701 tggcgggcag cgatggcatg agcattgtcc ccatgcgtgg tgatatcaac atcggttaat
     2761 gtaacgctgg cattggtata atcaagcgtg tcaccagcct tgacgacagt gccattagcg
     2821 tacgcggcat aactttctgt tccgtgggtt gtgatctccc cttttgccat atccagtttt
     2881 gctatggcat tattttcatt acttcctgta tcggtagtac tttcttcatc aagagaagat
     2941 acacacgcac gcgcgcttcc gggagatgca cacgcaagaa cgccgactgc gtgattaccc
     3001 gcagtaacaa taaatgtatc atctccgtca gaaaaagagg gggtaaccga accgtcagag
     3061 ttgactctta ttatggtgtc agtattcagg tttccactcg tttgacttag caaaccaatt
     3121 gagtaatccc ccgttatgac aatcgtgttt ttaccctgca aaataacagc accgccaaat
     3181 tcacttacga ttgcgctagc tgttaatgct gtgcttagag gactattgtc agaaggatat
     3241 aaaccgcccg ttgttacaat gacgccattg ttaatggtta acttgctacc ctctcctgca
     3301 acggataccc gtaacttcca ttacccgttg ttataactag cgcattatca atatctttta
     3361 cttttccatc ttttattgcc acagcatgtt tatattcatc gtgctgaata aactgttcag
     3421 catatgcatt acccaccgcc agagcaataa gtcccaaact aaacccggag agttttttca
     3481 gtttcataga ttaaccatcc attcattgga ttaacaaaca ttcctggtca aataacctat
     3541 gcgttcagat taccaaaacg gagggtaaag acaaaaacaa aagcacaaaa aatttaaata
     3601 gataaacagc gacaatatat aaatgataaa taagtcggca ttatcatttt tcaccttatt
     3661 ttcatgacat tgatcacttt agggtgacga ttttgtttaa caaataactc ttcgacatca
     3721 aaattgttaa tagttgcgat gaggattatt tcggtgatat ataaaaacaa ccgtgataac
     3781 accagggttg acaactattt gcgcaataat gcctttcctt caatgatcca ggcgttcact
     3841 gccagcaacc cattcagccg catactgaca gtgagaaact gaaagattat tgctgatccc
     3901 tgtggttgac gagtctttct cttaatcgat taaccagatt gatcgccagc caaacaaggg
     3961 cattaagcaa atttatcgtc acaagcactt ctcccagccc ggtactgggt tttgcaataa
     4021 ggatgaccag gagggtgatc agcataaatg caatggaaaa gatgatatag agagtgaaat
     4081 aatccagatt attactattg ttcatgctac taatggcaat cctgtcaaaa ctttcaaaca
     4141 attatagcaa acaaaataat gaacaatagt aacaagaggc aacgagctaa ttagctgtct
     4201 ataattacac cgcactatgg ccttactgat caattttcac tcaaccacaa ccttcacctt
     4261 cggtttagaa aggataattt tgaccaggtc gatgtatgta acaggtttat agaaataata
     4321 accctgctga aatgtgatat tattttggtt cagatagtca agttgttctt gcgtttcgac
     4381 accttcagcg acgatactga tcgaaagttt acgcgccaga tccaatacac aatccagaat
     4441 tcgggttgat tctgggtctg cgtttacgcg gccaacgaaa ctgtgatcaa ttttgatata
     4501 atcaatatgc aggtcatgaa gataagaaag ccctgagtag ccagtaccga aatcatccag
     4561 cgcgatgaca aaaccatttt catgcagaat gttcaaccgc tgaaccagac tttcatcaac
     4621 attcaatggc tcacgctcgg tgacttccag aacaaggttt aaatcgcggc gggtaaaact
     4681 gtcacggaaa tttaaacact cgtcgacaaa cgtcggcgaa ataatatgcg aagcgctaaa
     4741 attaattcca atatgaaaac cttccggcaa tttactcgcg atagcgttca tctgtctggc
     4801 aacctgattc atcaggcttt gcgtaagcgg aacgattaat ccggattttt cagcaagtgg
     4861 aataaatgcc gcgggtgata tatatccacc gtgaggttgt ttccagcggg ctaacacctc
     4921 aactccccgc aatgtccctt cccgaccatt taccacaggt tggtaaaaag ggacgatctc
     4981 cccattattt attgcccgtc gcaggatctc ttcaggcgtt gcgcttttat tgaaatatct
     5041 atcaagcagg aaggcagcgg cgcaggcgat taacagaata aatattaaaa ttccaaagcc
     5101 tttatcgata agtctgtaga agctaaagag tggcggcggg ttaaagataa tagtgaaatg
     5161 atattttgtg gagtgaacct tctcggtata gggataatgt ccattaagcg tcatcacatc
     5221 acccgttggt ccaataatgt catccgccac gcgtaataca taccttaccc ctttcaaggg
     5281 tacattaagc gccccgcgaa tatgttgatc acttatcgta accaaaatgc gcgtatctgc
     5341 aaattggctc tgatagagca ggataggtaa tctattaacg gtgtcgattg ccggagccaa
     5401 cagtaaatta ctgtccggga caacaggaat acgagacagc aggacccgat tcccaggcag
     5461 ggatgtacac cagactattc cctgtttgag aatgatgatt gtgcgcaggt gaggtttcag
     5521 agctgcttca gtgccaagct gatattgctc ctcgaggtcg cattccttcc cggcaatgtt
     5581 aaccgctgtt tgagtcgcct gtgacgcttc ttcaagtata tgattgatat tgttggcggc
     5641 atatctcgct cccgccaggt actctgcttt tgcggaatac cagagctgga tgtttaaaat
     5701 agcgacaccg gttataaaca agcatatcgc aacgaggatg ggtatgagtg tattgcgcat
     5761 ttgcttgtcc cggccaaagg gttttataca tgaaaatgcc attcattcgc tcgcgctggc
     5821 atctacagag agcatggttg agagcatagg aaatatccac gccgaagata aaattttctc
     5881 catcttgatc taacccatta attattgagc ataatgtagg caggattaaa taaattaggt
     5941 gggatatcag gcatcaagaa tggagaacga gaactctcca ttcttgacaa ctgatattgc
     6001 ggatatatta agaaagcata acgcctgaaa tgctcacttt gcaccagcat gatgatacag
     6061 ctgatgttta ttctataacc gtactcaagt tctaagagag cacggattcc ctgtcattaa
     6121 taatgatatc cgttagtgta gtcttatgca tttcaatgta ctgcaagatt tgcagataga
     6181 gatcagaaag aatcacactg gtttcatctt ttaaacgcaa gcgtaaagcc tgatcaataa
     6241 tatgactatc aacataccca tgcgtcatga acacctccct ctcgagattt ttaaacaaag
     6301 cacattgaaa aataaatctc tctgggattg aattattcat gt
//
LOCUS       DAZKIV010000203         6311 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZKIV010000203 DAZKIV010000000
VERSION     DAZKIV010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.205"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(111..784)
                     /locus_tag="ACHOT6_004339"
                     /pseudo
     CDS             complement(111..784)
                     /locus_tag="ACHOT6_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            861..1004
                     /locus_tag="ACHOT6_004340"
                     /pseudo
     CDS             861..1004
                     /locus_tag="ACHOT6_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1007..1150
                     /locus_tag="ACHOT6_004341"
                     /pseudo
     CDS             1007..1150
                     /locus_tag="ACHOT6_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1594..4581)
                     /locus_tag="ACHOT6_004342"
     CDS             complement(1594..4581)
                     /locus_tag="ACHOT6_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HFP9949446.1"
                     /translation="MARRQILSLSERESLLALPDDELTLTRMTYFSEQDLAIINAHRK
                     PASRFGFAVLLCYLKSIGFAPDKKSSPPAKLLKTIGARLKLSAELWQEYVSGRDTTRR
                     EHMRELYRYLELKAFNGALQKECIRHLLPLSTRTDRGILIAEELLKYLHQQRIIIPGI
                     DVLERTCAEAMTLGDKTVYTTLNAHLDITHKSALDALLVTNEKQISRLTWLLQPPGKI
                     NGKNVLQHIDRLNAIEALALPEGVERTVHQNRLLKLAREGRKMSSRDLADFSPARRYA
                     TMVCVLTEARATIIDEIIELHERILGSMFSRAKHQQAERLQKTGKLIQQKLRQYVSVG
                     QAILDARNAGEDPLSAIERVLPWDDFVASLEETKLLARKDNFEPLHLITEKFSTLRKY
                     SFRFLSALQLRATSAALPLNDALETIRDMYRKQLRKVPASAPLDFIPESWRKMVVTPS
                     GIDRQYYEICAMNELKGALRAGDIWVRGSRRYKNFDDYLMPNDDFEKLLNNNLLNLPV
                     GTECGAYLNARLTLLASRLEEVNAMAVAGELPDVDISDKGVKITPLDNSVPSTVSPLA
                     DLIYNMLPHPKITEILDEVERWTAFSRHFTHLKNPLIRPQDNRLLLTTILADGINLGL
                     AKMAESCPGTTRASLENIQAWYIRDETYSAALAELVNAQKSRPLAALWGDGSTSSSDG
                     QNFRVGSHGRYAGQVNLKYGQEPGVQFYTHISDQYSPFYTKVISRVRDSTHVLDGLLY
                     HESDLDITEHYTDTAGFTEHVFALMHLLGFAFAPRIRDLHDKRLFIQGKADNYPALQS
                     IISTTPLNIKEIKIHWNEILRLATSIKQGTVTASLMLKKLASYPKQNGLAKALREIGR
                     IERTLFMLDWFRDPALRRRVQAGLNKGEARNALARAVFMHRLGEIRDRGIENQSYRAS
                     GLTLLTAAITLWNTVYIENAINLLKKKGLHINEQLLSHLSPLGWEHINLTGDYIWKNN
                     KKLAEGKYRPLRAVDVSQYKKQP"
     gene            4748..5389
                     /locus_tag="ACHOT6_004343"
     CDS             4748..5389
                     /locus_tag="ACHOT6_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001722340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9949447.1"
                     /translation="MFIRAYLRASTEDQFADRAKEMLEQFVQERGHKIASYYRENISG
                     TKLDRPELKRLLMDSHRNDILLVEQIDRLTRLSNSDWITLKKQIEQHELRIVSLDVPT
                     SWQALSDKDPSQSDPITRAVITAINNMLIDLMAAMSHKDWLSRRQRQKQGIERAHTLG
                     KYRGKQADQERHQKVLYYRQVKKLSIRETADATGYSTSQVCRIQALYKEVKPD"
     gene            complement(5438..5787)
                     /locus_tag="ACHOT6_004344"
                     /pseudo
     CDS             complement(5438..5787)
                     /locus_tag="ACHOT6_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
     gene            complement(6033..6245)
                     /locus_tag="ACHOT6_004345"
     CDS             complement(6033..6245)
                     /locus_tag="ACHOT6_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFP9949448.1"
                     /translation="MNGFRNSSRNSQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     IAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT         1756 a         1520 c         1342 g         1693 t
ORIGIN      
        1 tcatttaaaa atcaaccaca acgcaaaaac aataaataag taataagtaa taagtaataa
       61 gtaataagta aaactcattt ttattaaaat aaaaacaaaa gaatatatcc ttaatcacaa
      121 tatccagatg ctcctcccca cgtaatatag acacaggccc aagcgaggtt ctggttttca
      181 aattgttccg gacagagacc gccacaccaa ctgtgccgtc gtcaccgatt gtaatcacat
      241 tcgatcagta gtccatatta tttcccggta gataaagtgt tctccatgga tacatttcac
      301 tttcagcgaa agaaagaagc tttccacgca ggcattatca ttgcagtaac cttttgcgct
      361 catactgccg tgtagattat gccgcttcaa cagcacctga taatctgcaa agcagtactg
      421 acctccacgg tccgtgtaaa cgataacgtt ccgggctatc ttacgcagcc acagcgccat
      481 ctgcagggaa tcgcaggcca cactgtcata catggcgaca tctaccagcg aataacgaca
      541 cgtgaccaca agtcaatgac cactgcctga tacagcccac cttcatctgt acgtaagttc
      601 gtgatgtctt ccgcccgttt ctggttcggt cactggcgta aaaatccagc tccagcagat
      661 tttctgacac aggcaggcca tgtgcacgat aacagaccaa gttgaacttc cgagaggcct
      721 ttgccttcag cccctgacag aacagactta ccaccatggt ttttacttta aagtggtaac
      781 cctgaactgt agtggtttac tgaatttggc cacctgaaaa gaggtaatat gctcacctca
      841 gtgacaatac aggtgcgcaa atgaaaagaa gaaattttag tcctgaattc aaacgagaat
      901 ccgctcaact ggttgttgac cagaactaca cagtggcaga tgccaccaaa gctatggatg
      961 tcggcctttc cacgatgaca agatgggtca aacacctgcg tgattagcgt cagggaaaaa
     1021 ccttgcggag gcattcgaac actataacaa atggcatccg cacagtgcac cgggttatcg
     1081 ctcaccgaga gaatatctgc ggcagcgggc cagtaacggg ttaagtgata acaggtgtct
     1141 ggaaatatag ggaaaatcca cagggtctat gtttgctcat taataattaa tatacgttct
     1201 ggcgattact tagtaatcgc cagctataga actctacgat ttttaagcaa gttaatggca
     1261 ctattctcgg cgttttcctt tgcttcctga tatgatttgc cccagacata attatccttt
     1321 ggtagatgaa tttcattccc gtcatagcca taaaacattt gtcacttttg cttcatattt
     1381 ttctccgcta gcgaaagatc tcattttgac tacagttgta gcattcttac ttgttcaaac
     1441 tcaaaagcca ttgctgctcc attaataact acctaagacg ttagaggatt aaatttcaat
     1501 ttaaacaatg aattatatcc ttaacgtacg tttccgttcc attagccctt cccccttgta
     1561 gggctccgtt tgggaaacgg aaaatattgt acgttaaggc tgttttttgt actgactgac
     1621 atcaactgct cgcaaaggac gatacttccc ttcagccagc ttcttattgt ttttccagat
     1681 atagtcccct gtcagattaa tgtgctccca gcccagcgga gacaaatgtg acaacaattg
     1741 ctcattaata tgtagccctt tctttttcag caggttaatg gcgttttcta tatatacggt
     1801 attccacaaa gtgattgcgg cagtgagcag cgtaagccca ctggcgcggt aactctggtt
     1861 ttcaatacca cgatccctta tttctcccag acgatgcatg aagacagctc tggcaagggc
     1921 attccgggct tcccctttgt tcagacctgc ctgaacccta cgacgaagcg ccggatcgcg
     1981 gaaccagtcc agcatgaaca gtgttcgctc aatacgacca atttctctta acgccttagc
     2041 aaggccattc tgtttcggat aactggccaa ttttttcagc atcagagaag cggtcactgt
     2101 tccctgtttt attgaggtcg ccagtctcag aatttcattc cagtgtattt ttatctcttt
     2161 gatattcagg ggggttgtgg agataattga ctgtagagca ggatagttat cagctttacc
     2221 ttgaataaac aaacgtttgt catgcagatc ccggatcctt ggggcaaaag cgaatcccag
     2281 cagatgcatt agtgcaaaaa catgctccgt gaaacctgct gtatcggtgt aatgttccgt
     2341 tatatcaagg tcactttcgt gatacagaag accatcaagt acgtgggtgg aatcacgaac
     2401 acgactgatt actttggtat aaaacggact atattgatct gagatatgcg tataaaactg
     2461 caccccaggt tcctgaccgt acttcaaatt aacctgaccg gcatagcgcc catgactgcc
     2521 aaccctaaaa ttctggccgt cagaggatga ggtggaaccg tctccccata atgccgccag
     2581 cggtcgtgac ttctgggcat taaccagttc tgccagtgca gcagagtacg tttcatcacg
     2641 gatgtaccat gcttgtatgt tttccagcga agctcgtgtg gttcccggac aggactctgc
     2701 catttttgcc aaccctagat tgatgccgtc cgccaggata gtggttagta gtaaacgatt
     2761 atcctgagga cggattagcg gattcttaag atgagtaaaa tgtcgcgaga aggcagtcca
     2821 tcgctcgact tcatcaagta tctctgtaat tttggggtgc ggtagcatgt tgtaaatcag
     2881 gtcggccagg ggagagactg ttgaaggaac gctgttatcc agtggggtga tctttacacc
     2941 tttatctgaa atatccacat caggaagctc ccctgcgaca gccattgcgt taacttcctc
     3001 cagacgggag gccagtagag tcagtctggc attaagataa gccccgcatt ctgtgccaac
     3061 aggtaaattt aataggttat tatttaataa cttttcgaaa tcatcgttag gcataagata
     3121 atcatcaaag tttttgtagc gccgggagcc cctgacccag atatcacccg cccgcagcgc
     3181 cccctttagc tcattcattg cgcaaatttc atagtattgt cggtcaatgc ctgagggggt
     3241 gaccaccatc tttctccagc tctcaggaat gaaatccagt ggtgccgatg caggaacctt
     3301 tcgtagctgt ttacggtaca tatcacgaat agtttcaagg gcatcattaa gcggcaacgc
     3361 tgccgaagtg gctctcagtt gcagggccga tagaaaacga aaagaatatt tgcgcaatgt
     3421 actgaatttt tcggtaataa gatgaagcgg ctcaaaatta tccttacgtg caagaagctt
     3481 tgtttcctcc agactggcga caaaatcgtc ccacggtaac actcgttcaa tagcagacag
     3541 aggatcttcg cctgcattgc gtgcatccag gatagcctgg ccaacagaaa catactgacg
     3601 taacttttgc tgtatcaact tgcccgtttt ctgaagacgc tcggcctgct gatgttttgc
     3661 ccgactgaac atgctgccta gaatacgttc atgcagttct ataatttcat caatgattgt
     3721 ggctctggcc tcagtcagta cacagaccat cgttgcgtat cgacgagcag gggaaaaatc
     3781 agccagatcg cgactgctca ttttgcggcc ctcacgcgcg agtttcagca atcggttctg
     3841 gtgaacggtt ctctccactc cttccggcag cgccagagct tcaatagcgt taaggcggtc
     3901 aatgtgttgc aggacattct ttccattgat tttccctgga ggttgaagca gccacgtcaa
     3961 acgggatatt tgtttttcat ttgtcaccaa cagggcatca agcgcagatt tatgcgtgat
     4021 atcaaggtgc gcattcagcg ttgtatacac cgttttatca cccagtgtca ttgcttctgc
     4081 acaggtacgt tccagaacat caatgcctgg aataataatt cgctgttgat gcaggtactt
     4141 tagtaactcc tctgcgataa gtatgcctct gtccgttctg gtagacagag ggagcagatg
     4201 ccggatacat tctttctgaa gtgcaccgtt aaaggctttc agttcaagat atcgataaag
     4261 ttccctcata tgctcacgtc tggtggtatc acgaccggaa acgtattcct gccaaagttc
     4321 tgctgataac ttaagtcgag cgccaatggt ttttagcaat tttgcgggtg gggatgattt
     4381 tttatccggg gcaaacccaa tacttttcag ataacagaga agaacggcaa aaccaaaccg
     4441 gctggcaggt ttacggtgag cattgataat tgccagatcc tgctcactga aatacgtcat
     4501 tcgagtcagt gttaactcat cgtctggcaa tgccagtaat gattctcttt ccgatagaga
     4561 aagaatctgc ctccttgcca tatagttatc cttgttaatt atttagtacg ttatttttga
     4621 tgaaaaggtt attgcataag ggtacaccct gatgcgagat ataaaatgtg acctcataca
     4681 aataatgaca taacctatat gcgatagaat atatgcaaca acctaaatgc gacaatgcgg
     4741 tgaaaaaatg ttcatcagag cttatttaag ggcgtcaaca gaagatcagt ttgcagatcg
     4801 agcaaaagag atgctggaac agttcgtcca agaacggggg cataaaattg ccagttatta
     4861 tcgggagaac ataagcggta caaaacttga ccgtccggaa ctgaaacgct tactgatgga
     4921 cagtcaccgt aatgatattt tgctggtcga gcagatagac cgcctgaccc gactgagtaa
     4981 cagcgactgg ataacgctga aaaagcagat agaacaacac gaactgcgga ttgtcagtct
     5041 ggatgttcca acatcatggc aagccctgtc agataaagat ccttcacaat cagatccgat
     5101 tacccgcgca gtgataactg caataaataa tatgcttata gacctgatgg ccgcgatgtc
     5161 gcataaggac tggctgagtc gtcgccaacg ccaaaaacag ggaattgaac gagctcatac
     5221 gttgggtaaa taccggggaa aacaggccga tcaggaacgt catcagaaag tgttgtacta
     5281 cagacaagta aaaaaactca gcattagaga aacagcagat gcaacgggct acagcacttc
     5341 gcaggtttgt cgcattcagg ctctctataa agaggttaaa cctgattaaa aaacagcctt
     5401 aacgtacaat attttccgtt tcccaaacgg agcccttgta ctgctcatat acccaggcag
     5461 ccccgacccg aaccataaac tgattaacat tcattccgtc tggtgcataa acctgcccga
     5521 gaatacgacc ataacgatct cgctgaaaat atgtgacggt aaccgttttt cccgcaatca
     5581 gtgatttcat catgtctgtt gaccaccgac cataatcctg tttcttttca ggtgcatcaa
     5641 tatttaccag cctgatccgc accgccttca gtgagtccat tacctcgatg gtatcaccgt
     5701 ccagtacacg aagccccgtc catgaatatc tgcagacaat accggccatg aaaagataaa
     5761 taataccagt ggaatatatt tcctcatgtt attctccata tcagataaat ggcacctgta
     5821 tttcagcccc cctgaatagt gcagcacaca ttgaataaca ccttattttg tgaaattaat
     5881 cacaaaatat aaaatcacga cttaatacag aaaaaactct gtgctactca aaactgtccg
     5941 gtcatactgc ccgaccggat attaaaaaaa gcaaatctca taagctcctt tcagccttct
     6001 gacttctggt gtaatgaagg ctgctttttt cgttacaccc ttccccggca gcgccggtgg
     6061 cagtgttcag cccttttccg ggcaaactgc tgccagtctg tccggggagc tatgcaggca
     6121 gcccgtcgcc atgcttctgc cgcttccatc cagcgtccac tgacctccag aataacggcc
     6181 cgcccgccgg cacgctggta acgccagacc tgactgttcc gtgaactgtt tctgaatcca
     6241 ttcatcttta tcactcctct ttaacccgtc agtattatgg tgttaaataa tctactgaat
     6301 tggcttcctt t
//
LOCUS       DAZKIV010000204         6162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZKIV010000204 DAZKIV010000000
VERSION     DAZKIV010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.206"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..1096
                     /locus_tag="ACHOT6_004346"
     CDS             47..1096
                     /locus_tag="ACHOT6_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFP9949449.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            1170..2027
                     /gene="nfo"
                     /locus_tag="ACHOT6_004347"
     CDS             1170..2027
                     /gene="nfo"
                     /locus_tag="ACHOT6_004347"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFP9949450.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            2031..3119
                     /locus_tag="ACHOT6_004348"
     CDS             2031..3119
                     /locus_tag="ACHOT6_004348"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFP9949451.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISCSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3175..4425)
                     /locus_tag="ACHOT6_004349"
     CDS             complement(3175..4425)
                     /locus_tag="ACHOT6_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFP9949452.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(4525..5466)
                     /gene="rihB"
                     /locus_tag="ACHOT6_004350"
     CDS             complement(4525..5466)
                     /gene="rihB"
                     /locus_tag="ACHOT6_004350"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFP9949453.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            5596..>6162
                     /locus_tag="ACHOT6_004351"
     CDS             5596..>6162
                     /locus_tag="ACHOT6_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFP9949454.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAVFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYV"
BASE COUNT         1537 a         1567 c         1512 g         1546 t
ORIGIN      
        1 ttaaaccagt tagtcgtaac cttatgcccg gttaaaggag agggttatga cgaatatcac
       61 cttacagaaa caacatcgta cactgtggca ttttattccg gggttagccc tgagtgcagt
      121 tatcaccggg gtcgccctgt ggggtggttc cattcccgcg gttgcgggtg ccgggtttag
      181 tgccctcacc ctcgcaatct tgttggggat ggttttaggc aacaccattt acccgcacat
      241 ctggaaaagc tgtgacggtg gtgtgctgtt tgccaagcaa tatttattac gtctgggtat
      301 cattctttat ggcttccgtc tgacgttctc gcaaattgcc gatgtcggta tcagtgggat
      361 catcattgac gtgctgacgc tgtccagtac cttcctgctg gcttgcttcc tggggcagaa
      421 agtgtttggt ctggataagc acaccagctg gttgatcggt gccggtagca gtatctgtgg
      481 tgctgccgcg gtactggcga ctgagccggt agtgaaagcg gaagccagta aagtaaccgt
      541 ggctgttgca accgttgtta tcttcgggac cgtcgcgatt ttcctctacc cggcgatata
      601 tccgctgatg tctcaatggt ttagtccgga aactttcggt atctacattg gttctactgt
      661 gcacgaagtg gcgcaggtgg tggcggcagg tcatgccatc agcccggatg cggaaaacgc
      721 agcagttatt tccaaaatgc tgcgcgtgat gatgctggct ccgttcctca tcctgctggc
      781 ggcgcgtgtt aaacagctgt ctggggcgaa cagcggcgag aaaagcaaaa tcactattcc
      841 gtggtttgcc atattgttca tcgtcgttgc catctttaac tcgttccacc tgttaccgca
      901 gagcgtggtg aacatgctgg taacgctgga taccttcctg ctggcaatgg cgatggcggc
      961 gctgggtctg accactcacg tcagcgcgct gaaaaaagcc ggggcgaaac ctctgctgat
     1021 ggcactggta ctgtttgcct ggctgattgt tggtggtggt gcgattaact atgtgattca
     1081 aagcgtcatc gcataaacca ctacatcttg ctcctgttaa cccgctatca ttaccgtttt
     1141 cctccagcgg gtttaacagg agtcctcgca tgaaatacat tggagcgcac gttagtgctg
     1201 ctggcggtct ggcaaatgcc gcaattcgcg ccgccgaaat cgacgcaacc gcgtttgcct
     1261 tgttcaccaa aaaccaacgt cagtggcgtg ccgcaccgct cacgacgcaa accatcgatg
     1321 aattcaaagc ggcctgtgaa aaatatcact acacatcggc gcaaattctt ccccacgaca
     1381 gctatctgat taacctcgga catccggtca ctgaagctct ggaaaaatcg cgcgatgcct
     1441 ttatagatga aatgcagcgt tgcgaacaac tggggctttc tttgctcaac ttccaccctg
     1501 gcagccatct gatgcagatt tcagaagagg attgccttgc gcgtattgcc gaatccatca
     1561 acattgcgct ggataaaact caaggtgtaa cagcggtgat tgaaaacacc gccggtcagg
     1621 gcagtaactt agggtttaaa ttcgaacatc tcgcggcgat tatcgacggc gtggaagata
     1681 aatcccgtgt cggcgtctgc attgatacct gccatgcttt cgctgccggg tatgatttgc
     1741 gtactccagc cgaatgcgag aaaacgttcg cggattttgc ccgtattgtt ggctttaagt
     1801 atctgcgcgg gatgcacctt aacgatgcga aaagcacctt tggcagccgc gttgaccgcc
     1861 atcatagcct cggtgaaggc aatatcggtc atgatgcgtt ccgctggatc atgcaggacg
     1921 accgtttcga cggcattccg ctgatccttg aaaccatcaa cccggatatc tgggcagaag
     1981 agatcgcctg gctgaaagcg caacaaactg aaaaagcggt ggcctgatcg atgaacgaca
     2041 gggaaaagca gattcttaaa attttacggc gtaacccgct gattcaacag aatgaaatcg
     2101 ccgatattct gcaaatcagc tgctcacgtg ttgccgctca tattatggat ttgatgcgca
     2161 agggtttgat taaaggcaaa ggatatatcc ttaccgagca ggattactgt gtggttgtgg
     2221 gtgcaatcaa tatggatatt cgcggaatgg cggatatccg ttacccgcaa gcagcctctc
     2281 atccaggttc tgttcactgt tccgcaggcg gcgtcggacg taatatcgcg cataacctgg
     2341 ctctgcttgg tcgggatgtg catttaatat cagctattgg caacgatttt tacggcgaga
     2401 cattactgga agaaacgcgc cgcgcgggcg tgaacgtttc aaactgcatt cgtctgcatg
     2461 gacacagcac tgcaacttat ctcgctatcg ctaataagca ggaagaaact atcctggcaa
     2521 tcaatgacac acatattctg caacagctaa cgcctcaact gctcaatact tcacgggact
     2581 tgattcgcca tgctggtgtg gtgctggctg attgtaatct cacacctgaa gccctggagt
     2641 gggtctttac cattgctgat gaaattccga tgtttgtcga taccgtttcc gaatttaaag
     2701 cgaacaaagt aaaaaactgg tacagccgga tccacactct gaaaccaaca caaaatgaac
     2761 tggaaatttt gtgggggcaa ccgattaaag acgacaacga tcgcatcaga gctgtaaatt
     2821 cgctgcatca acaaggtgtt aaacgcattt ttgtttatct gaaagatgaa tctgtatttt
     2881 gcagcgataa ggatggagaa caatttttgc tgaccgcgcc agcgcatacg acagtagaca
     2941 gttttggtgc tgacgatggt tttatggcgg gcctggtata tagctttctt gaaggataca
     3001 gtttccgcga cagcgcccgt tttgcggtag cctgcgcggc aatttcgcgc gccagcggca
     3061 gcttaaacaa ccctaccctg tctgccgata acgcgctttc attagtgcca atggtgtaac
     3121 aatgttgccg gattcgacgc ttgaccgcat cttatccggc tcataaattg cgcactacgc
     3181 taaaccaata aagaacccgg caatggtggc actcatcaga ttagaaagtg tcgccgccgc
     3241 cagcgcgcgt aaaccaagct gggcgatttc cggcgcacgg tgtggcgcaa ccgcagaaaa
     3301 cgcccccacc accaccccga tagaaccaaa gttagcgaaa ccgcacaacg cgaaggaaat
     3361 aatcgccacg gttttagcat ccagagtgcc agccgtttgc agatagggtg agaaattgag
     3421 ataagcgaca aattcattta ttgccagttt ctgtccaatc aaactcccgg caagatttgc
     3481 atcactccag tcaacaccca ttacccacgc aagtggggcc agcaggtaac ctaaaatgga
     3541 ctccagcgag gcatgttcaa aaccaaacca gccgccaacg ccgccgataa taccgttaat
     3601 taacgcaatg atggcgacaa atgccataac aactgtcgct acaccggcgg cgattttcag
     3661 cccggtcatt gcccctgtcg cagcggcttc aataatgctt tttggcggtg tttcggtgaa
     3721 agagaggtta ttaaaggaaa cctgagaaga ttccgttgcc ggacttaaca ggcgggcaaa
     3781 caagatcccg ccagggatcg ccattaacga tgccgccagc agatattcca caggcacgcc
     3841 cagtgcggcg taaccaatca ttgtcgaacc agcaatcgag gccatgccac tacaaatcgc
     3901 tgtaaataat tcattgcgat tcagccgatc gataaagggt ttgacgattg ccggaatttc
     3961 gttttgcccg aggaaaatgg tagtgaccgc gacgaatgac tcgatcttgc tgatatttaa
     4021 cgctttctgg aagataccgc cgagaatgcg aattaaaatc cccatcacac cgatgtagta
     4081 gagaatactc accagtgctg taacgaagat aattgccggt aatactctga aaccaaagat
     4141 aaatcctgcg ccatcaaaca gcgtgtccat tttcggcccg accagcgaac cgaagataaa
     4201 tgcgctaccc gcgtcgctgt acgccatcac tttatgcacg ccaaaagcga ctttttcagc
     4261 gacccaacgc cctggcggta accaaagcat aatgccgcca atcacaacct gtaacactaa
     4321 cgccgcgcca acggtacgca ggctgatctt cttcttgttt actgacagca aaaatgcaat
     4381 tgccagcaat accaccattc ccagaacact tctcatgaca tccatagtga ttatctcttc
     4441 atgtcatatt gggcgacggc tttctgccca tcagaaatag ccgggcctgc atcttgcgct
     4501 atcggcccgg cttgttatca acgcttaatg ggttttgatg tagccgcgca cgcactcttc
     4561 gactaagccc cagaaccagt cggtatcaat agtgatgccg actttggtat tggcgggctt
     4621 gccaagaacg cccagctcgt cgcagacggt gcgcccatag caagggccac tgttgacgtc
     4681 cacttcgacg tacatctcct gggttttaat gccatcaggg ttaatcagat aaccgatgca
     4741 ggtggcgtcg tgcaccgggc cgccagccag gccgtagttt tcgaactgcg ttttgagagt
     4801 gaagttcatg atgtcgctga acagctctcc ggcagggccg cctgcccttt ccatccgagc
     4861 aatcacgtcc ggggtgcaaa cggtctggtt ggtgagatcg aggcccatca tcactaatgg
     4921 aacgccagag gtgaacacca cgcgggctgc ttccgggtca gcaaagatgt tgaactcggc
     4981 agatggcgtg aagttgcctg taccgtaagc gccgcccatc agcacaattt cacggatttt
     5041 gggcaggatc gcgggttgca tacgcattgc caccgcgata tttgaaagcg gaccaaccgg
     5101 caccaaagtg atatcgccat cgcttgccat cagggtatca atgatgtatt tcaccgcgtg
     5161 ggtactttct gcctggcggg tcagcggctc gaataccggg ccatccagtc cggtttcacc
     5221 gtgaatatta tcggcaacga tttgtttacg cataatgggc tgtggcatcc ccgcataaac
     5281 cggaacatta atctccagtt tctggcaaac attcaggcca ttaattaatg ttttatcgag
     5341 ggtttgatta cccgcaacaa tggtgatacc taataaatca atcgccggat gtttcgccgc
     5401 catcattata gcaatagcat catcatgacc cggatcacaa tccagaataa tttttctctt
     5461 ttccattgtt tatttcctct gtttccagtt gcgttatttt ttctacagca aagaaaattc
     5521 gaccgggccg atgattgaat cttaacaaca gcgtacgtat gctaaatatg agaaatctca
     5581 tagcggatat acatcgtgaa agaaatccac aataatgatc ttaagcagca attgatgagt
     5641 gaatccgcgt ttaaggattg ctttttaacg gatgtttcag ccgatacgcg gctgtttcat
     5701 tttttagcgc gtgactacat tgtgcaggaa gggcaacagc cgtcctggct gttttacctg
     5761 acgcgaggcc gcgccaggct ttacgccacg ctagctaatg gtcgcgtatc gctgatcgat
     5821 ttctttgccg ccccctgttt tattggcgag attgagttaa tcgataaaga ccatgaaccg
     5881 cgtgcggtac aggctattga agagtgttgg tgccttgcgc tccctatgaa acattaccgt
     5941 ccactgttat taaacgatac gctattttta cgaaaactct gcgtcacctt aagtcataaa
     6001 aattatcgta atattgtttc tttaactcag aatcaatcat ttccgttagt gaatcgcctg
     6061 gcagtattta tattactctc gcaggagggt gatctttatc acgaaaaaca tacccaggca
     6121 gcagagtatt taggcgtttc ttatcgacat cttttatatg tt
//
LOCUS       DAZKIV010000205         6130 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZKIV010000205 DAZKIV010000000
VERSION     DAZKIV010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6130
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.207"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..851
                     /gene="exuR"
                     /locus_tag="ACHOT6_004352"
     CDS             75..851
                     /gene="exuR"
                     /locus_tag="ACHOT6_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFP9949455.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1091..1174
                     /gene="yqiM"
                     /locus_tag="ACHOT6_004353"
     CDS             1091..1174
                     /gene="yqiM"
                     /locus_tag="ACHOT6_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFP9949456.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1196..1858
                     /gene="yqjA"
                     /locus_tag="ACHOT6_004354"
     CDS             1196..1858
                     /gene="yqjA"
                     /locus_tag="ACHOT6_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFP9949457.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1862..2245
                     /gene="mzrA"
                     /locus_tag="ACHOT6_004355"
     CDS             1862..2245
                     /gene="mzrA"
                     /locus_tag="ACHOT6_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFP9949458.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2392..2760
                     /gene="yqjC"
                     /locus_tag="ACHOT6_004356"
     CDS             2392..2760
                     /gene="yqjC"
                     /locus_tag="ACHOT6_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFP9949459.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2798..3103
                     /gene="yqjD"
                     /locus_tag="ACHOT6_004357"
     CDS             2798..3103
                     /gene="yqjD"
                     /locus_tag="ACHOT6_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFP9949460.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3106..3510
                     /gene="yqjE"
                     /locus_tag="ACHOT6_004358"
     CDS             3106..3510
                     /gene="yqjE"
                     /locus_tag="ACHOT6_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFP9949461.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3500..3799
                     /gene="yqjK"
                     /locus_tag="ACHOT6_004359"
     CDS             3500..3799
                     /gene="yqjK"
                     /locus_tag="ACHOT6_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFP9949462.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3985..4377
                     /gene="yqjF"
                     /locus_tag="ACHOT6_004360"
     CDS             3985..4377
                     /gene="yqjF"
                     /locus_tag="ACHOT6_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFP9949463.1"
                     /translation="MKKLEDIGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4447..5433
                     /gene="yqjG"
                     /locus_tag="ACHOT6_004361"
     CDS             4447..5433
                     /gene="yqjG"
                     /locus_tag="ACHOT6_004361"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFP9949464.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5726..6091
                     /gene="yhaH"
                     /locus_tag="ACHOT6_004362"
     CDS             5726..6091
                     /gene="yhaH"
                     /locus_tag="ACHOT6_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFP9949465.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1531 a         1517 c         1546 g         1536 t
ORIGIN      
        1 atatatgacc aatggcatac ccatgaataa agtggtatga taacttcgtc cgcattccac
       61 tccgggagac gcatatggaa atcactgaac cacgccgttt gtatcaacaa cttgccgctg
      121 acctgaaaga gcgcatcgaa cagggcgtct atctggtagg tgataaactg cctgcagaac
      181 gctttattgc cgatgaaaag aacgtcagcc gtacggtagt tcgtgaagcc atcatcatgc
      241 tggaagttga aggctatgtc gaagtgcgta aaggttcagg tattcatgtg gtttccaacc
      301 agccgcgcca tcagcaggcg gctgacaata atatggaatt cgccaattac ggtccgtttg
      361 agttgcttca ggctcgccag ctcatcgaaa gtaatattgc cgagttcgcg gcaactcagg
      421 taacgaaaca agacatcatg aaactgatgg ccattcagga acaggcgcgc ggcgaacaat
      481 gcttccgtga ttccgagtgg gatttacagt tccacattca ggtagccctg gcgacgcaga
      541 actccgccct ggcggccatc gttgaaaaaa tgtggaccca acgtagccat aacccgtact
      601 ggaaaaaact gcacgaacac attgattccc gtaccgtcga taactggtgt gatgaccacg
      661 atcaaatcct caaggcgctg attcgcaaag atcctcatgc tgctaagctg gcaatgtggc
      721 agcacctgga aaacaccaag atcatgttat tcaacgaaac cagcgacgac ttcgagttca
      781 atgccgaccg ctatctgttc gccgaaaacc cggtagtgca tctcgatacg gccaccagcg
      841 gcagtaaatg aaatacctgt ccgacaggcg ctgcgacgcg cctgtttgtc tcgtcggtaa
      901 gcgaaaagta taaagtgtca gcctgtgtaa atcctctcgc caccctcccc tgcattcagc
      961 aaaatcagac tccacggaca tgtaattttg ataacgaaca acgttgacct ttgttacaat
     1021 tagattcaat ttgaatttat gtttttgaat gctttcttat ctcacgattt aacagggaat
     1081 agttcaggct gtgttgatgt atcaaacccg cagaacatac caaaacagca ataacattgc
     1141 ggtagtgcat cttttaaaac cagcgtggcg ttaaccgatt caccaggaat aatgaatgga
     1201 acttttgacc caattgctgc aagccctgtg ggcgcaggat tttgaaaccc tggccaatcc
     1261 atcgatgatt ggcatgttgt attttgtctt gtttgtaatt ttgttccttg aaaacggctt
     1321 gcttccagcg gcctttttac cgggcgacag tttactggta ttggtcggcg tgttgattgc
     1381 gaaaggcgcg atgggttacc cgcaaacgat tctgctgctg accgttgccg ccagcctcgg
     1441 ctgctgggtc agctatattc aggggcgatg gctgggcaat acccgcaccg tacaaaactg
     1501 gctatctcat ttacccgcgc attatcatca acgcgcacac catctttttc ataaacacgg
     1561 tttatcggcg ctgttaattg gtcgctttat tgcgtttgtc agaacactgc tgccgacgat
     1621 tgccgggtta tcagggctga ataacgcgcg ctttcagttt ttcaactgga tgagcggtct
     1681 gctgtgggta ttgatcctga caactctggg ttacatgctc ggcaaaacgc cggtattttt
     1741 aaagtacgag gaccagctga tgtcatgcct gatgctgctc ccggtggtgc tgctggtgtt
     1801 tggcctggca ggttctctgg tcgtgttatg gaaaaagaaa tatggaaatc gggggtaagg
     1861 gatgcaaata cctcgcatgt cgcttcgcca gctagcctgg tccggcactg ttttacttct
     1921 ggtcggcacg ctgttgctgg cctggtccgc ggttcgccag caagagtcta cgctggcgat
     1981 tcgtgccgtt catcaaggca caacgatgcc agacggtttt tcgatctggc atcaccttga
     2041 cgctcatggc attcctttca aaagtatcac ccccaaaaac gacactctgt taattacatt
     2101 tgactccagc gaccagagcg ccgccgcaaa agcggtcctc gacagaacat tgccccatgg
     2161 ctacatcatt gcgcagcagg acaataacag tcaggctatg cagtggctga cccggttacg
     2221 ggataactct catcgcttcg gataacttcc tgttatccga aacatatcac tcactttggt
     2281 gatttcaccg taactgtcta tgattaatga ggcggtggtt aacactaccg cttcgttgtc
     2341 tggacaatcg ttcctttgta ataggtccga atcacaatgg aaggttcaag aatgaaatac
     2401 cgcatcgctt tagctgtttc tctctttgct cttagtgccg gtagttatgc cactaccctg
     2461 tgtcaggaaa aggagcaaaa tatccttaag gagatcagct atgccgaaaa acaccaaaac
     2521 cagaatcgta ttgacggtct gaataaagcc ctgagtgaag tccgggccaa ctgttcagat
     2581 agccagctgc gtgccgatca tcacaagaaa atcgcaaagc agaaagatga ggtggcggaa
     2641 cgccagcaag atttagccga ggcgaagcaa aaaggcgatg ccgataagat tgccaaacgc
     2701 gaacggaaac tggcagaagc gcaggaagag ctgaaaaagc tggaagcgcg cgactactaa
     2761 ctcacaatag tcactactta ctcacctgga gaaaactatg tcgaaagaac acactacgga
     2821 acatctgcgt gctgagttga aatccctttc cgatacgctg gaagaggtgc ttagctcatc
     2881 tggcgagaag tcgaaagaag agttgagtaa gattcgtagc aaagcggagc aggcactgaa
     2941 acagagccgt tatcgcctgg gtgaaaccgg tgatgccatc gccaaacaaa cccgtgtcgc
     3001 ggcggcgcgt gctgatgagt atgtgcgtga aaatccgtgg accggcgtgg gcattggcgc
     3061 tgcaatcggt gtagtgctcg gcgttctgct gtcgcgtcgt taattatggc ggacactcat
     3121 cacgcgcaag ggcccggtaa aagcgttctg ggcatcgggc agcgaattgt ttccatcatg
     3181 gttgaaatgg tggaaacacg tctgcggctg gcggtggtgg agctggaaga ggaaaaagct
     3241 aatctctttc aacttttact gatgctgggc ctgacgatgc ttttcgctgc atttggtctt
     3301 atgagcctga tggtgctaat tatttgggcg gttgatccgc aatatcgcct gaatgcgatg
     3361 attgccacca ccgtggtgtt gctgctactg gcactgattg gcggtatctg gacgttacgt
     3421 aaatcgcgta aatccacctt gctgcgccat acacgccatg agctggcaaa cgatcggcaa
     3481 ctgctcgagg aggagtcccg tgagcagtaa agtcgaacgt gaacgacgta aggcgcaact
     3541 gcttagccag atccagcaac aacggctgga tctttccgcc agtcgtcgtg aatggctgga
     3601 ggcaacaggc gcttatgatc gtcgctggaa tatgctgcta agtctgcgct cctgggcgct
     3661 ggttggcagt agcgtgatgg cgatctggac gattcgccat cctaatatgc tggtccgctg
     3721 ggccagacgc ggttttggcg tatggagcgc ctggcgtctg gttaaaacga ccctcaagca
     3781 gcaacagctt cgcggttaac ttccctctgg ccggagcaat tccggcctta tccctcaaat
     3841 tttttgaaga tttttgacag ttttccttgc taacaatcat cattcaccac gtttatgatt
     3901 ctctccatcg acagcaacga cgctaatacc gcgcccttgc acaaaaaaac aatcagcagc
     3961 ctgagtggcg cagtggagag tatgatgaaa aaattagaag atattggtgt actggtagcg
     4021 cgcattttaa tgccgattct gtttattacc gcaggctggg gaaaaatcac cgcttactct
     4081 ggtacccaac aatatatgga agcaatgggt gtcccgggtt ttatgctgcc gctggtgatt
     4141 ctgcttgagt tcggtggtgg tctggcaatc ctgttcggtt tcctgactcg caccacagcc
     4201 ctgtttactg cgggctttac gctgctgacg gcatttttat ttcacagcaa ctttgctgaa
     4261 ggcgtcaact cgctgatgtt catgaaaaac ctgacaattt ctggcggatt cctgctgctg
     4321 gcaattaccg gtccgggcgc gtatagcatc gaccgcctgc tgaataaaaa gtggtaagca
     4381 cgcgctatac ttaacgataa aaaacgagga ggaagctcct cgtttttgct attggaggag
     4441 agaaaaatgg gtcaactgat tgacggcgtc tggcatgaca cctggtacga taccaaatct
     4501 accggcggta aatttcaacg ttcagcttcc gcatttcgta actggctcac tgccgatggc
     4561 gcacctggcc ccactggcac aggcggtttt atcgcagaga aagatcgtta tcatctctat
     4621 gtttcactcg cctgcccgtg ggcgcaccgc acgctgatca tgcgcaaact caaaggactg
     4681 gaaccgttta tttccgtttc cgtagtgaac ccgctgatgc tggaaaacgg ctggaccttt
     4741 gatgacagtt ttccgggagc aaccggagat acgctctatc aacatgaatt tctgtatcag
     4801 ctttatctcc acgccgatcc acactacagc ggacgagtta ctgttcccgt gctgtgggac
     4861 aaaaagaacc acaccatcgt cagcaacgaa tcagcagaaa tcatccgcat gttcaatacc
     4921 gcgtttgatg cgctgggcgc aaaagcgggt gattactacc caccagccct gcaaacgaaa
     4981 attgacgaac ttaacggctg gatttatgac accgttaaca acggcgtgta taaagctggt
     5041 tttgccacca gccagcaagc ctacgacgag gcggtggcga aagtgtttga atcgctggcg
     5101 cgactggagc agattttagg ccagcatcgt tacctgaccg gcaaccagct aaccgaagcc
     5161 gatattcgcc tgtggaccac gctggtccgt tttgatccag tgtatgtgac ccacttcaag
     5221 tgtgataagc accgcatcag cgattacctt aatctgtatg gcttcctgcg tgatatctac
     5281 cagatgccgg gaattgccga aacagtcaat ttcgatcaca tccgtaatca ttacttccgc
     5341 agccataaga ccatcaaccc tacggggatt atttcaattg gtccatggca ggatctcgat
     5401 gaaccgcatg gacgagatgt tcgcttcggt taaaaaaagg caccttacga ggtgcctctg
     5461 ataattagct aagtgcatgt tgttcatgtc ggatgcggcg tgaacgcctt atcaaaccta
     5521 caaaacattt atattcaata tgttgcaatg accgcgcagg ccagataagc gtagcgcatc
     5581 tggcgatttt gcgcttgtca tcagtcttag cgcgccttca cagcacttta ttaattcctt
     5641 gaacgaatat ttactgccat ttcattcacg tctattctta atttgctgct ttaaaaacaa
     5701 gtgattgagc aaaattgagg caaaaatgga ctggtatctg aaagtactaa aaaattatgt
     5761 cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg tttattctgg tcaacatcat
     5821 ctttacgttc gtgctggggc tgctggataa aatgttaggc tggcaacgcg ctggtggcga
     5881 aggcatcctg acgacaatct acggtattct ggtgttttta ccgtggtggg cagttcagtt
     5941 ccgccgcctg cacgacaccg accgctcggc gtggtgggca ctgttgttct taatcccgtt
     6001 tatcggctgg cttattatca tcgtctttaa ctgccaggcg ggtacgccag gcgaaaaccg
     6061 ctttggacca gatccgaaac tggaacctta aaacaaatct gctcgccgga tgtctgactc
     6121 atccggcttt
//
LOCUS       DAZKIV010000206         6098 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZKIV010000206 DAZKIV010000000
VERSION     DAZKIV010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6098
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.208"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..911
                     /locus_tag="ACHOT6_004363"
     CDS             <1..911
                     /locus_tag="ACHOT6_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFP9949466.1"
                     /translation="RALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHA
                     TLKEIKDLAKNITQQYGTPDLCIEAMRKKNLKTMLFISQLKAKTNFQVVGLTMKCYSP
                     VCKNASRMDQNLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSY
                     IALNLEGSSQERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYR
                     LSLSPSIKRSAAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSES
                     VVVGQKIDNISLDEFAKALKSVLARI"
     gene            complement(1173..>6098)
                     /locus_tag="ACHOT6_004364"
     CDS             complement(1173..>6098)
                     /locus_tag="ACHOT6_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9949467.1"
                     /translation="TASVSSGSQANQQVIFIGDQSTAALTLSVPSGDITVTNTAPLHM
                     TATLQDKNGNPLKDKEITFSVPNDVASRFSISNSGKGMTDSNGTAIASLTGTLAGTHM
                     ITARLANSNVSDTQPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVV
                     KNLSVVFRTSPADTQLSLKALNTNENGIAEVTLKGTVLGVHTAEAILLNGKSDTKIVN
                     IVPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPLAGITVNFTIPQDVAANF
                     TLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNASDAQPVTFVADKDSAVVVL
                     QTSKAEIIGNGVDETTLTATVKDPFDNAVKDLPVTFSTNPADTQLSQSKSNTNDSGVA
                     EVTLKGTVLGVHTVEATLPNGNGYTTTVNIAPDASNAQVTLNIPAQQVVTNNSDSAQL
                     AATVKDPSNHPVAGITVNFTMPQGVAANFTLENNGIAITQANGEAHVTLKGKKAGTHT
                     VTATLGNNNTSDSQPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNEV
                     NNLPVTFSSASSGLTLTPGVSNTNESGIAQATLAGVAFGEQTVTASLANNGASDNKTV
                     HFIGDTAAAKIIELTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAA
                     TAEMTNGGQAVTNEQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNAD
                     ASTAHLTLLQALFDTVSSGDTTNLYIEVKDNYGNGVPQQEVTLRVSPSEGVTPSNNAI
                     YTTNHDGNFYASFTATKAGVYQVTATLENGDSMQQTVTYVPNVANAEITLAASKDPVI
                     ADNNDLTTLTATVADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTL
                     KGTKAGAHTVTASMTGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATV
                     TDGNGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEILVTSTEVGLKTVSASLADKPT
                     EVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVKAHVNDQFGNPVAHQPVTFSAE
                     PSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKASLANGASLEKQLEAIDEKLTLT
                     ASSPLIGVYAPTGATLTATLTSANGTPVEGQVINFSVTPEGATLSGGKVRTNSSGQAP
                     VVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSSTAHVASFIADPSTIAATNTDLS
                     TLKATVEDGSGNLIEGLTVYFALKSCSATLTSLTAVTDQNGIATTSVKGAMTGSVTVS
                     AVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGDYTDSAELRLVLHDISGNPIKV
                     SEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTGGGEGIATLIPVLNGVHQAGLS
                     TTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAADYEFS
                     SSASWVDVDATGKVTFKNVGSNWERITATPKSGGPSYVYEIRVKSWWVNAGEAFMIYS
                     LAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYWSSSPAN
                     SNEQYVVSLATGDQSVFEKLGFAYATCYKNL"
BASE COUNT         1431 a         1450 c         1473 g         1744 t
ORIGIN      
        1 ttcgggcgct tgaattgcat aaaattaagc atctattaat tgttacgtcg acaattaatt
       61 tagaagtctt taatgcgctc aaacttgaac agactaaatt aacattagtc acaatgacta
      121 tgcaggatca cgcaacatta aaagaaataa aagatttagc gaagaacata acacagcaat
      181 acggtacgcc ggatctttgt atagaggcga tgcgtaaaaa gaacctgaaa acgatgttat
      241 ttatcagtca gttgaaagca aaaacgaatt ttcaggttgt tggtttaacc atgaagtgct
      301 attcccctgt gtgtaagaac gcgtcccgga tggatcagaa tctccgggct cccgtaccca
      361 tgacatgggc atttatgatg cgtgaggcgg gttttccagc agtcaggcca atatatgaat
      421 tgccactaag tgaggatgta ctcgatgagg tacgcgagga aatgcgatcg ttaggatctt
      481 acattgcgct taatttagaa ggtagctcgc aggaacgtac attttcacta tcgattgcag
      541 aaaatctaat agcaaaaatt caaagtgaaa cagatatacc aatagtgatc gttcatggac
      601 caaaagggga agataaagcc agggtattag tggattgtta taataatgtc taccgtttat
      661 ccttatcacc ctcgattaaa cgttcagcag caatcataaa agatgcttat atcgcaataa
      721 ctcctgacac ctcaatatta catatggcaa gtgcctataa tacccccgtt gttgcaattt
      781 atgctgatta caaaacgcga tggcccgcaa tggccgatgt ttcggagtca gtcgtcgttg
      841 ggcaaaaaat tgacaatata agtctggatg aattcgcaaa ggcattaaaa agtgttttgg
      901 cgagaatatg agacagtact tatgcatagt aatgccgatc tgttatgaac ggtggtgtca
      961 tcaatactct gaggctggtc aatcatcaca gaaaaccagc ctttggatca aagcagtact
     1021 caccggatac gatcatagtc acaccgaacg cgattcttgc ggtgcagcat ttttatttat
     1081 tcattttctg tgagagaagg gcaagctcta tagagggaat gtaaaaaaga gtgctccgaa
     1141 tggagcactc tttaagaaat tataacggaa aatcagaggt ttttataaca tgtcgcataa
     1201 gcaaacccaa gcttttcaaa tacgctttga tcacctgttg ccagggaaac tacgtattgt
     1261 tcgtttgagt ttgcgggact agatgaccaa tacatatttg attgaaaacc agcttcagtc
     1321 gtgtaatgcc ccatatctcc ccattcactg tacagtgacc cgatgcctcg ggaactactg
     1381 tggtttaaat agtttgctct ggggagcgtg tagccattgc tgctgcaaaa attttcagca
     1441 aggctgtata tcatgaaagc ctcgccggcg ttcacccacc aactcttcac acggatttcg
     1501 tatacatagc tagggcctcc tgattttggc gtcgccgtaa tcctttccca attgctgccg
     1561 acatttttaa atgtcacttt accggtagca tcaacatcga cccaggaggc agagcttgaa
     1621 aactcataat cagccgccgt ttttcctggg gcaaagttgt cattattcaa ctgataatac
     1681 gccccggtga acccctgcga agggaatgta gttgtcggta ggtcagtacc attgactgat
     1741 actgtaccgc tcattatttt gtcttctgcg cgagtgaatt gtattgtggt actcagacca
     1801 gcttgatgaa caccattcaa tacagggatc agcgttgcga taccctctcc gccgcctgta
     1861 acagtggctt tgtaatcacc gttgatattt agactgtaat caattgcgct aatttttata
     1921 tagggcacgt tagtacctga ttgcacaaat tccatccctt cagaaacttt gatcggattg
     1981 cctgatatat cgtgcagaac aagacgtaat tcagcactat cggtatagtc ccctttcagt
     2041 gatgaccgat tgctcttaag gacggactgc gaggtgtctg ccgggccagc caccagcgtt
     2101 atatctactg tttgcattcc accagcggtc gtgactgcgc ttaccgtgac gctacctgtc
     2161 atcgctcctt tcacgcttgt tgtcgcgatt ccgttttgat cggtcaccgc tgttaatgac
     2221 gttaatgtgg cagagcagct ttttaaggcg aagtacacag tgagaccttc gatcaggtta
     2281 ccactgccat cctcaaccgt tgcctttaag gtacttaaat cagtgttggt ggcggcgata
     2341 gtcgatggat cagcgataaa gctagcaaca tgggcggtgc ttgagtttcc agtgactttc
     2401 acggttgtct gtgtctgtat tgttacgccg ttatggaacg atgcagtcac cgtatatgta
     2461 ccgactttat tgctggtcag aacgactgga gcctgacctg aagagttagt tctcactttt
     2521 ccgccactta acgtcgcccc ttctggcgtt acgctaaagt tgatgacctg accctccact
     2581 ggagtgccat ttgcagaggt tagcgttgcc gtcagagtag cacctgtagg ggcatagaca
     2641 ccgataagcg gactggacgc cgtgagtgtc agtttttcat caatagcctc cagttgtttc
     2701 tcaagtgagg ctccattcgc cagggatgct ttcaccatat acgaaccgtt tctttcgggc
     2761 gtcatggtga cctcggctac accctgcgta ttagtagaaa ccgtattctg gctaatgatc
     2821 atttgcgagg atggctctgc actgaatgtc acgggttgat gcgcaaccgg gttgccaaac
     2881 tggtcgttaa cgtgagcttt aactgctacg tcttgtgcga ccattacctg accttcaggt
     2941 atctccagac tggtaatcgt cgcagaatta acatctgcac tggcattcag taatcgcgag
     3001 atgacttcag taggtttatc tgccagagag gctgaaactg ttttcagtcc gacctcggtg
     3061 cttgtcacaa gaatttcagc gaaaccccga tcatcagttt caacgctggt gctgcttagc
     3121 gtgacggagg ttccgcggaa attaactttt atgccttcta ccgggttgcc gtttccatca
     3181 gtcactgttg cctgcagcct ggtcatcccg acgttattag cgataaaatt gtcctcggta
     3241 acgttaagat taacctgcgc agtgagcgta tccgcaataa agttcaccac caactgctca
     3301 ctcttaccgc cagtcatcga tgctgtaaca gtatgagcgc ctgcttttgt acctttcagc
     3361 gtgactttcg ctttgccttc agcatcagta atcgctttac cgccatcgct cagcgtgaag
     3421 ttcgccttca catcttccgg cagagtaaat gttacttcag tgttggctat cgcattgccc
     3481 tctgtatcag cgactgttgc tgttagtgtc gtgagatcgt tattgtcggc aatcaccgga
     3541 tccttcgagg ctgccagcgt gatttcggca ttcgcgacgt tcggcacata ggtcactgtt
     3601 tgttgcatcg aatcgccatt ttcgagggtt gccgtcactt gataaacccc ggcttttgta
     3661 gcggtaaagc ttgcgtaaaa attgccgtcg tggttggtag tatatatagc gttattactg
     3721 ggggtcacgc cttcacttgg tgatactctg agggttacct cctgctgggg tactccgttg
     3781 ccgtaattat ccttcacctc aatatacaga ttggtagtgt cgcctgagga gactgtatca
     3841 aaaagtgcct gtagcaaggt gagatgtgcc gtagacgcat cagcgttgac attaattgat
     3901 gtgctaagcg tggagctacc attttccaga ctggcctcaa cggtatccgg tctcgctcct
     3961 gattctatcg aggagcgggt attggtataa gtgacggtag ccttaccctg ttcgttcgtc
     4021 acggcttgac cgccgttcgt catttcggct gtcgctgcgt tgctggtgaa gttcacagtc
     4081 acacctttca ccggaaagcc attattatca acgactgtgg cggtgatgac gctgccggag
     4141 ctgttctgcg gggtaccggc gattatgctg tctgggacag gcgtcaactc gataattttt
     4201 gccgccgctg tgtcgccaat aaaatgcaca gttttgttgt cgctggcacc attattagcc
     4261 agtgatgcag tgaccgtctg ctcaccaaag gcaacgcctg cgagagtggc ctgcgcgatg
     4321 ccagactcat tggtattact tactcccggg gtcagggtga gtcctgaaga ggctgagctg
     4381 aatgttaccg gaagattatt cacctcattg tcgaactgat ctttaaccgt tgcagttagc
     4441 gttgcgttat cgacgccatt acctgtgatc tcatcttttg acatctgcag gacaacctga
     4501 gccgaggttt tgtccgccac aaatgttacc ggctgcgaat cactggtatt gttattaccc
     4561 agcgttgcgg taaccgtatg cgtacccgct tttttacctt tgagcgtgac atgcgcttcc
     4621 ccattggctt gggtgatggc aatgccgtta ttttcgaggg taaagtttgc cgccacgccc
     4681 tgtggcatgg tgaagttcac cgttattccc gccaccggat gattcgacgg atctttcacc
     4741 gttgccgcca gctgcgcgct gtcactgtta ttcgtcacca cctgttgagc agggatgttc
     4801 agcgtaacct gcgcgttgct ggcatcgggc gcaatattga ctgtcgttgt atagccgttg
     4861 ccgttaggaa gtgtggcttc gactgtatga acgcccaaaa ccgtgccctt aagggtaact
     4921 tcagcaacac cactgtcgtt agtattgctt ttgctctgac taagttgagt atctgccggg
     4981 ttggtactga aggtgaccgg tagatctttt actgcgttat caaaaggatc tttcactgtt
     5041 gccgtcagag tcgtctcatc cacgccattc ccaatgattt ccgctttcga tgtttgcaga
     5101 acgacaactg cgctgtcctt atccgccacg aatgtgactg gttgcgcatc gctggcatta
     5161 ttgttaccca acgttgcggt aaccgtatgc gtgcccgctt ttttaccttt gagggtgaca
     5221 tgcgcttcgc cattggcctg agtgatggcg atgccgttat tttcgagggt aaagtttgct
     5281 gccacgtcct gtggtatggt gaagttcacc gttattcccg ccagcggatg attcgacgga
     5341 tctttcacca tcgccgtcag ctgtacgctg tcgctgttat tcgtcaccac ctgttgagcc
     5401 gggatgttca gggtgacctg cgcgttgctg gtatcgggca caatattgac gattttcgta
     5461 tcgcttttac cgttaagcag tatggcttcg gctgtatgaa cacccaaaac cgtgccttta
     5521 agggtaactt cagcaatacc gttctcatta gtattaagcg ccttcagact cagttgcgtg
     5581 tctgcggggg aggtgcggaa gactactgac agatttttaa ccacgttatc aaaaggatct
     5641 ttcactgttg ctgtcagagt cgtctcatcc acgccattcc caatgatttc cgctttcgat
     5701 gtttgcagaa cgacaaccgc tctgtcttta tcggccacaa acgtcattgg ctgtgtatcg
     5761 ctgacattgc tgttagccag acgagccgtg atcatatgcg tgcccgctaa cgtgccggtc
     5821 agggaggcga ttgcagtccc gttgctatcc gtcatgcctt ttccgctatt gctaatcgag
     5881 aaccgacttg cgacgtcgtt tggcacagag aaggtgattt ctttatcttt taatgggttg
     5941 ccatttttat cctgcaaggt tgcagtcata tgtagcggag ctgtgttggt gacggtgata
     6001 tcacctgaag gcacactgag ggtcagggca gcagtacttt gatcaccgat aaaaatcacc
     6061 tgttgattag cctgagaacc agagctcaca gaggccgt
//
LOCUS       DAZKIV010000207         6037 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZKIV010000207 DAZKIV010000000
VERSION     DAZKIV010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.209"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            203..1306
                     /gene="ompC"
                     /locus_tag="ACHOT6_004365"
     CDS             203..1306
                     /gene="ompC"
                     /locus_tag="ACHOT6_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFP9949468.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1418..2473
                     /gene="apbE"
                     /locus_tag="ACHOT6_004366"
     CDS             1418..2473
                     /gene="apbE"
                     /locus_tag="ACHOT6_004366"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFP9949469.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2547..3611
                     /gene="ada"
                     /locus_tag="ACHOT6_004367"
     CDS             2547..3611
                     /gene="ada"
                     /locus_tag="ACHOT6_004367"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFP9949470.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPVDLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3611..4261
                     /gene="alkB"
                     /locus_tag="ACHOT6_004368"
     CDS             3611..4261
                     /gene="alkB"
                     /locus_tag="ACHOT6_004368"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFP9949471.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILHRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4337..5980
                     /gene="yojI"
                     /locus_tag="ACHOT6_004369"
     CDS             4337..5980
                     /gene="yojI"
                     /locus_tag="ACHOT6_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFP9949472.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
BASE COUNT         1434 a         1525 c         1631 g         1447 t
ORIGIN      
        1 taatcttaaa aagttccctt ctatttacat tttgaaacat ctatagcgat aaatgaaaca
       61 tcttaaaagt tttagtatca tattcgtgtt ggattattct gcatttttgg ggagaatgga
      121 cttgccgact gattaatgag ggttaatcag tatgcagtgg cataaaaaag caaataaagg
      181 catataacag agggttaata acatgaaagt taaagtactg tccctcctgg tcccagctct
      241 gctggtagca ggcgcagcaa acgctgctga agtttacaac aaagacggca acaaattaga
      301 tctgtacggt aaagtagacg gcctgcacta tttctctgac aacaaagatg tagatggcga
      361 ccagacctac atgcgtcttg gcttcaaagg tgaaactcag gttactgacc agctgaccgg
      421 ttacggccag tgggaatatc agatccaggg caacagcgct gaaaacgaaa acaactcctg
      481 gacccgtgtg gcattcgcag gtctgaaatt ccaggatgtg ggttctttcg actacggtcg
      541 taactacggc gttgtttacg acgtaacttc ctggaccgac gtactgccag aattcggtgg
      601 cgacacctac ggttctgaca acttcatgca gcagcgtggt aacggctttg cgacctaccg
      661 taacactgac ttcttcggtc tggttgacgg cctgaacttt gctgttcagt accagggcaa
      721 aaacggtagc gtaagcggcg aaggcatgac caacaatggt cgcgatgctc tgcgtcagaa
      781 cggcgacggc gttggcggtt ctatcactta tgattacgaa ggcttcggta tcggtgctgc
      841 agtttccagc tccaaacgta ctgatgctca gaacaccgct gcttacatcg gtaacggcga
      901 ccgtgctgaa acctacaccg gtggtctgaa atacgacgct aacaacatct acctggctgc
      961 tcagtacacc cagacctata acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     1021 acagaacttt gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtctgtagc
     1081 atacctgcag tctaaaggta aaaacctggg taccatcgct ggtcgtaact acgacgacga
     1141 agatatcctg aaatatgttg atgttggtgc tacctactac ttcaacaaaa acatgtccac
     1201 ctacgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     1261 caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc
     1321 gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaactc atttttttgg
     1381 tgtactcttg cgaccgttcg catgaggata atctcgtatg gaaataagct ttacccgcgt
     1441 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     1501 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     1561 cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     1621 gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     1681 taacgactcg caaagtttgt cgccgtggcc agtaagtgaa gcgatggccg aaatcgtcac
     1741 tacctcgctg cgcattggcg cgaagaccga tggtgcgatg gatattaccg tcgggccgct
     1801 ggtgaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     1861 gatcgatgcg atgaaagcca aaaccggctt acagcacctg acggtcatca atcagtcgca
     1921 tcagcaatat ctgcaaaaag acctgccgga tttatatatc gatctctcca ccgtcggtga
     1981 aggttatgcg gcggatcacc tggcacgctt gatggagcag gaagggattt cccgctatct
     2041 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatgaac ggtgaaggcc agccgtggcg
     2101 ggtagcgatt caaaaaccaa ccgataaaga aaacgcggtt caggctgtgg tggatatcaa
     2161 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     2221 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     2281 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     2341 cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     2401 caaagaaggc gatagcttta aaacctggat gtcgccgcag tttaaaagct tccttatcag
     2461 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     2521 ggctatcctt aaccagggag ctgattatga aaaaagccac atgcttaact gacgatcaac
     2581 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
     2641 gtaccacagg catcttttgc cgtccgtctt gccgcgccag acatgctttg cgggaaaacg
     2701 tctccttcta cgcaaatgcc agcgaggcac tcgccgctgg ctttcgcccc tgcaaacgtt
     2761 gtcagccaga caaagccaat gctcagcaac atcggttgga taaaatcacc cacgcctgtc
     2821 gactgctgga acaggaaacg cctgtaacgc tggaagcctt agccgaccat gtggcgatga
     2881 gtccgttcca tctgcatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
     2941 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
     3001 cgtctattct taacgccgga ttccccgaca gcagcagtta ctatcgcaaa gctgacgaaa
     3061 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
     3121 cgctggctga ttgtgagctg ggtcgttgcc tggtggcaga aagcgagcgg gggatttgcg
     3181 cgatattgct gggcgatgat gacgcgacac taatcagcga gttgcagcag atgtttcccg
     3241 ctgccgacaa cgcgcctgtc gatctgatgt ttcagcaaca tgtgcgtgaa gtgatcgcca
     3301 gcctcaatca acgcgatacg ccgctgacgt taccgctgga cattcgcggc actgcttttc
     3361 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgtc agttatcagc
     3421 aactggctaa cgccatcggc aaaccgaaag cggtacgggc cgttgccagc gcctgtgccg
     3481 ccaacaagct ggctatcata ataccctgtc atcgggtggt ccgtggtgat ggcacacttt
     3541 ccggttaccg ctggggcgtg tcgcgtaaag cgcaactgct gcgccgcgaa gctgaaaatg
     3601 aggagaggta atgttggatc tgtttgccga tgctgaaccg tggcaagagc cactggcggc
     3661 tggagcggta attttgcacc gttttgcttt taacgctgcg gagcaactaa tccgcgatat
     3721 taatgacgtt gccagccagt cgccgtttcg ccagatggtc acccccgggg gatataccat
     3781 gtcggtggcg atgaccaact gtgggcatct gggctggacg agccatcggc aaggttatct
     3841 ctattcgccc attgatccgc aaacaaataa accgtggccc gccatgccac agagttttca
     3901 taatttatgt caacgtgcgg ctacggcggc gggctatcca gatttccagc cagatgcttg
     3961 tcttatcaac cgctacgctc ctggcgcgaa actgtcgctg catcaggata aagacgaacc
     4021 ggatctgcgc gcgccaattg tttctgtttc tctgggctta cccgcgattt ttcaatttgg
     4081 tggcctgaaa cgtaatgatc cgctcaaacg tttgttgttg gaacatggcg atgtggtggt
     4141 atggggcggt gaatcgcggc tgttttatca cggtattcaa ccgttgaaag cggggtttca
     4201 tccactcacc accgactgcc gctacaacct gacattccgt caggcaggta aaaaagaata
     4261 aaaataagaa ttattattgc tgtgcgcgcg aagatgttta aactgcgggc tgtaattcat
     4321 tgtccgggtt ttctgcatgg aacttcttgt acttgtctgg cggcagtatc gctggccatt
     4381 tatcagtgtg atggcgctaa gcctcgccag tgcggcatta ggcattggct taattgcttt
     4441 tatcaatcag cgccttatcg aaacggcgga taccagtctg ctggtgttgc cggagtttct
     4501 tggattatta ctgctgttga tggcagtcac tctcggatcg caactggcgc tcaccacttt
     4561 ggggcatcac ttcgtttacc gactgcgtag tgaatttatc aagcggattc tggataccca
     4621 cgtcgagcgc attgaacaac tcggtagcgc ctcgttgctg gcgggattaa ccagcgatgt
     4681 gcgcaatatc accattgctt ttgtgcgtct tccggaactg gtgcagggga tcattctcac
     4741 tatcggttcc gcggcgtatc tgtggatgct gtcgggcaaa atgttgctgg taacggctat
     4801 ctggatggcg atcaccatct ggggtggttt tgtactggtg gcgcgggtgt acaaacatat
     4861 ggcgaccctg cgtgaaaccg aagacaagct gtacacggat tttcaaaccg tactcgaagg
     4921 gcgcaaagag ctgactctga accgggaacg cgccgagtat gtgtttaaca acctctacat
     4981 ccccgatgcg caagagtatc gccatcatat tattcgcgca gacaccttcc atcttagcgc
     5041 cgtgaactgg tcaaacatca tgatgctggg cgctatcggc ctggtgttct ggatggcgaa
     5101 cagtctcggt tgggctgata ccaacgttgc tgcgacctat tcgttgacgc tgttattcct
     5161 gcgtacgccg ctgctttcgg cggttggcgc attgccgacg ctgctgacgg cgcaggtggc
     5221 gtttaacaag ctgaacaaat tcgcgctcgc gcctttcaaa gctgagtttc cgcgcccgca
     5281 ggcttttccc aactggcaaa cgctggagct gcgtaacgtg acgtttagct atcaggataa
     5341 cgcgttttcc gttgggccga ttaacctcac cattaaacgt ggcgagctgc tgtttctgat
     5401 tggtggcaac ggtagtggaa aatcgacgct ggcgatgttg ctgacgggct tgtaccagcc
     5461 acaaagcggc gaaatcttgc tggatggcaa acctgtcagt ggcgaacaac cggaagatta
     5521 tcgcaaactg ttttcggcag tgtttaccga tgtctggctg tttgatcaac tgctggggcc
     5581 ggagggtaaa cccgctaacc cgcaactggt tgagaagtgg ctggcgcagc tgaaaatggc
     5641 tcataagctt gagttaagca acgggcgtat tgttaacctg aagttatcaa aagggcagaa
     5701 aaaacgcgtg gcgctgttgc tggcgctggc agaagaacgc gatattatcc tgctggatga
     5761 atgggcggcg gatcaggatc cacacttccg tcgtgagttt tatcaggtgt tgctgccgct
     5821 gatgcaggag atgggtaaaa ctattttcgc tatcagtcat gatgatcatt actttatcca
     5881 cgccgaccgc ctgctggaaa tgcgcaatgg gcaacttagc gagctgacgg gcgaagagcg
     5941 cgatgccgct tcgcgtgatg ccgttgcccg gacggcataa tattacgacg ctgacctggc
     6001 ggactgctgc cgtcaggtca atatgctaaa aatccct
//
LOCUS       DAZKIV010000208         5890 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZKIV010000208 DAZKIV010000000
VERSION     DAZKIV010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.210"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..412)
                     /locus_tag="ACHOT6_004370"
     CDS             complement(<1..412)
                     /locus_tag="ACHOT6_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFP9949473.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPL"
     gene            730..1371
                     /gene="udk"
                     /locus_tag="ACHOT6_004371"
     CDS             730..1371
                     /gene="udk"
                     /locus_tag="ACHOT6_004371"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFP9949474.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERIKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            1463..2044
                     /gene="dcd"
                     /locus_tag="ACHOT6_004372"
     CDS             1463..2044
                     /gene="dcd"
                     /locus_tag="ACHOT6_004372"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFP9949475.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            2066..3919
                     /gene="asmA"
                     /locus_tag="ACHOT6_004373"
     CDS             2066..3919
                     /gene="asmA"
                     /locus_tag="ACHOT6_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFP9949476.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4193..5776)
                     /gene="yegH"
                     /locus_tag="ACHOT6_004374"
     CDS             complement(4193..5776)
                     /gene="yegH"
                     /locus_tag="ACHOT6_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFP9949477.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
BASE COUNT         1464 a         1496 c         1583 g         1347 t
ORIGIN      
        1 acagaggcgg aacaatggcg ctgccgagtg ccagacgcag ccagtcagcc agattttgca
       61 aggggttata ccacggcagc aatttacgta gcagcactgc cccgaccacg gcttcaacaa
      121 tattgatggt cgtccaggtc atgttcagcg agctggtgga aaaaagcagg atggatgcgg
      181 cgatatttcc cagcgagcag ctcagcgcaa ttcccggcca catgcgcccg gcatggcgat
      241 aaaacgccac catcatgatg gacgtcggga accacagtgg ggcgagttgg gtgccaaact
      301 gcgaaagctc aagcgagaaa agggtaaaga taaacgagac taaacctaaa ctgaccaggt
      361 gaagcagcgg gtggggcagg gcaattaata catgctgtga ttgtttgctc attaccgctt
      421 tatccgcaag accaggtcgc cagtattcgt ttctgtgttt atgctagtac aaaagtttta
      481 cattttatat gcgaattgct cataaagtga cgtaaaggag gataatttgc gcaactgcgt
      541 ataacatttt ttaccttgta taaaactgat caacgtaatt tgccaccggg aattcttcgt
      601 caacaattaa ctctgagcgc gcggcaacgc tattcgactg gtatcaggcg gatgaaatcc
      661 ctataattgc cgcgtttggc gcttcgtcgc ccccttccta acatccaggt taatcaggtc
      721 gctaaatata tgactgatca gtctcatcag tgcgtcatta tcggtatcgc tggcgcatcg
      781 gcttccggca agagtcttat tgccagtacc ctttatcgtg aattacgtga gcaagtcggt
      841 gatgaacaca tcggcgtaat tcccgaagac tgctattaca aagatcaaag ccatctgtcg
      901 atggaagaac gcattaagac caactacgac catcctagcg cgatggatca cagcctgttg
      961 cttgagcatt tacaagcgtt gaaacgcggc tcggcaattg acctgccggt ttacagctat
     1021 gttgaacata cgcgtatgaa agaaacggtg acggttgagc cgaagaaagt catcattctc
     1081 gaaggcattt tgttgctgac ggatgcgcgt ttgcgtgacg aacttaactt ctccattttc
     1141 gttgataccc cgcttgatat ctgcctgatg cgccgcatca agcgtgacgt taacgagcgt
     1201 gggcgttcaa tggattccgt gatggcgcaa tatcaaaaaa ccgtgcgccc gatgttcctg
     1261 caattcattg agccttctaa acaatatgcg gacattatcg tgccgcgcgg cgggaaaaac
     1321 cgcattgcga tcgatatatt gaaagcgaaa ataagtcagt tctttgaata agcttgataa
     1381 attgtgtacc gttcagtgat aacctggtat gcccttgacg taattggcgt taagggagtg
     1441 atgcgcgaaa ggagaaaatg ccatgcgtct gtgtgaccga gatattgaag cctggcttga
     1501 tgaaggccgt ttgtcgatca acccacgtcc gccagtggag cgtattaacg gcgcgacggt
     1561 ggatgtacgc ctgggcaata aatttcgtac cttccgtggt cacacggcag cgtttatcga
     1621 tctgagcggt cccaaagatg aagtgagcgc cgcgcttgac cgcgtgatga gcgatgagat
     1681 cgttctcgac gagggcgagg cgttctatct tcacccagga gagctggcgc tggcggtgac
     1741 gctggagtcg gtgacgctgc cagccgatct ggtgggctgg ctggacgggc gttcctcttt
     1801 ggcgcgtctg gggcttatgg tgcacgtcac cgcgcaccgc atcgatccgg gctggtctgg
     1861 ctgcattgtg ctggagttct acaactccgg taagctgccg ctggcgctgc gtccgggcat
     1921 gttaattggt gcgctgagct ttgagccgct ttccggcccg gcggcgcgac cttacaaccg
     1981 ccgtgaagat gcgaaatatc gcaaccagca gggcgcggta gccagccgaa tcgataaaga
     2041 ctaattcagg cccattgagg ataccatgag acgatttctg acgacgctga tgatactcct
     2101 agtcgtgctg gtggccgggt tatctgcgtt agtgttgctg gtgaatccga atgatttccg
     2161 cgactatatg gtcaagcaag ttgctgcacg tagcggttat caattgcagc tcgacgggcc
     2221 actgcgttgg cacgtctggc cgcagcttag tatcctctcc gggcgaatgt ctctcaccgc
     2281 ccagggcgca agccagccac tggttcgcgc cgacaacatg cgtctggacg tggcgctttt
     2341 accactactg agtcatcaac tgagcgttaa gcaggtgatg ctaaaagggg cagtgatcca
     2401 actgacgccg cagacggaag cggtgcgcag tgaagacgct ccggttgcac cgcgcgacaa
     2461 taccttgccg gatctgtcag acgatcgcgg atggtcgttt gatatatcca gtcttaaggt
     2521 ggcggacagc gtgctggtgt tccagcatga agatgacgag caggtgacaa tccgcaatat
     2581 ccgcctgcaa atggaacaag atccccaaca tcgtggctca tttgagttct ccgggcgggt
     2641 taatcgcgat cagcgcgatc tcacaatatc ccttaacggt acggtggatg cttctgatta
     2701 tccgcatgat ttaacggcgg ctattgaaca aattaactgg cagttgcagg gtgccgattt
     2761 accaaaacaa ggtattcagg ggcaggggag tttccaggcc cagtggcagg agtcacataa
     2821 acgcctttca tttaaccaaa tcagcttgac cgccaatgac agtacgctga gcgggcaagc
     2881 acaggtcacg ctgacagaga aaccggaatg gcagctgaga ctgcaattcc cgcaactgaa
     2941 tcttgacaac ctcatcccgc ttaatgaaac cgcgaatggt gaaaacggtg ccgcgcagca
     3001 ggggcagagc caatcaacgt tgccgcgccc ggtcatttct tcgcgtattg atgaaccggc
     3061 ctatcaggga ctgcaaggct ttacggctga tattttgttg caggccagta acgtgcgctg
     3121 gcgcggaatg aattttacag atgttgccac gcaaatgacc aacaagtcgg gtttgctgga
     3181 aattactcaa ctgcagggca aacttaacgg tggacaggtt tcactgccgg gcacgctgga
     3241 cgcgacatca ataaatccgc ggataaactt ccagccacgg ctggaaaacg ttgagattgg
     3301 taccattctg aaggcgttta actatccgat ttcgttgacc ggaaaaatgt cactggctgg
     3361 tgatttctcc ggtgctgaca tagatgccga cgcattccgc cacaactggc aaggacaggc
     3421 acatgtcgaa atgaccgaca cgcgcatgga agggatgaac ttccagcaga tgattcagca
     3481 agcggtagag cgtaatggcg gtgatgtgaa ggccgctgaa aactttgata acgtgacgcg
     3541 gcttgaccgc tttaccaccg atttgacgtt gaaggatggc gtcgtgacgt taaacgacat
     3601 gcaaggtcaa tcgcctgtgc tggcgctgac aggggaaggc atgttgaatc tggcagatca
     3661 aacctgcgat acccagtttg atattcgggt cgtgggtggc tggaacgggg aaagtaaact
     3721 gattgatttc ctgaaagaaa cgccagtacc gctgcgggtt tatggcaact ggcagcaact
     3781 caattacagc ctgcaagtgg atcagttact gcgcaaacat ctacaggacg aagcgaaacg
     3841 tcgcctgaat gactgggccg agcggaataa agattcccgc aatggcaagg atgtgaagaa
     3901 gttgctggag aagatgtagg ttaccaggtg accagtcctg taggcctgat aagacgcgta
     3961 aagcgtcgta tcaggcgttc gtggcggcat cattatattc tgaatatgta agatcgaagg
     4021 ctgttccgtt cacttgatgt tcctcgctac tatggaacgc tcaagccggt gaacgtgcca
     4081 gggtggctca atcgccacca ccctggcaac ccgggctccc ggcggtaaat cgccgctggc
     4141 gcggttccct catcttattc ctttcggcta ttgggtaaaa tggccgatac catcacacct
     4201 cgtactccat ctcgccatct ttacgcagtg gaataatctg cactttctgc acgcgatggc
     4261 tttccacctg caacgtttta agcaaataat cccccacctg aacttcttcg ccaggttttg
     4321 gaatacgctg taaatattcc atcaacagcc cggcaatggt gtgatattca cgtttttcat
     4381 ccagcggcag cggcacatat tgcaccagat cctccagcgg catatgacca ttcgccgtcc
     4441 aggaaccgtc cgcattcttc tgaatatcat ggcgggcgtc gatctcttcc acttcgttcg
     4501 gtaagttacc ggcaatggtt tcagtgacgt cacttaatgt cacaatccct tccaccgagc
     4561 caaactcatc caccacaaaa gcaaagtgcg tgcgggcatt acggaactgc tccagggcag
     4621 gtaacaacgg caaggtttcc gggaacacca gcggctggcg aatcaacacc cgcaggttga
     4681 gcggttcgcc gcgcagagac tgttgtagca ggtcgataac gtgaacaaca ccgagcaaat
     4741 cttctgcgtc atcaccgtcg gtaaccacca gccgcgtatg ctgatttcgc tccagtaact
     4801 ggcggatctc gtcttccggc gcgttgagat cgatatgctc aatatcgtgg cgcgacgtca
     4861 taatgctgct gacggtacgc tggttaagat taagtacccg ctcaatcatc cgccgttcct
     4921 gcggattaaa gatctgctgg ttaccatgat ccaccagcat ggacgcggtt tcggcgtcca
     4981 gttccgcatc ttctttttgc ccgctaagca ggcgcattac cgcttcggta gtccgctggc
     5041 gcagcgtctg gttagcggaa agaaaacgtc gccggttaaa gatagccaac tgattgagcg
     5101 cctcgatcat caccgagaaa ccaatggcag cgtacaggta gcctttcgga atgacgaagc
     5161 cgaaaccttc tgccaccagg ctaaagccaa tcatcaacag gaagcttaag cagagaataa
     5221 cgatcgtcgg gtgactgtta acaaattgcg ttaacggctt gctggccatc aacatcaggc
     5281 tgatagcgat aaccacggcg gccatcatga ccagtaaatg gtcgaccatc ccgacggcgg
     5341 taatcactga gtcgagtgag aaaatggcgt ccagtaccac aatttgcgtc accacgcccc
     5401 agaacttcgc acctttgcgt tgtgtgggat tattgctgtc tttcccttcc agccgttcgt
     5461 tcagctccat cgtggctttg aacaacagga agaaaccacc aaacagcatg attaagtcgc
     5521 gggcgctaaa ggtaaacgag cggaagctga acagcggttg agtcagggtg accagccagg
     5581 agattgacgc cagcagtaac aggcgcatta acatcgccag cagcagcccg gtaacccgtg
     5641 cgcggtcgcg ctgcttcggc ggtagttttt cggcgaggat ggcaataaag accaggttat
     5701 caatgccgag gaccagttcg atcacaatca gcgtgattaa cccggcccag atagacggat
     5761 cggcaatcca ttccatagta agtacaaaac ctattcgttg tatgacaaat gtcacaattc
     5821 gatcatggat aaatcggggc aaaaatgcaa tgttgcagca ctatttatct catattattt
     5881 gagggtaata
//
LOCUS       DAZKIV010000209         5885 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZKIV010000209 DAZKIV010000000
VERSION     DAZKIV010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.211"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            69..1139
                     /gene="ygjJ"
                     /locus_tag="ACHOT6_004375"
     CDS             69..1139
                     /gene="ygjJ"
                     /locus_tag="ACHOT6_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFP9949478.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            1156..3507
                     /gene="ygjK"
                     /locus_tag="ACHOT6_004376"
     CDS             1156..3507
                     /gene="ygjK"
                     /locus_tag="ACHOT6_004376"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFP9949479.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            3833..5851
                     /gene="fadH"
                     /locus_tag="ACHOT6_004377"
     CDS             3833..5851
                     /gene="fadH"
                     /locus_tag="ACHOT6_004377"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFP9949480.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT         1424 a         1590 c         1633 g         1238 t
ORIGIN      
        1 tgcctttata tttttataat tacaacgata aaaggctgta ctttttcttt agctcatgga
       61 ttaacacaat gaaattaatc actgcaccat gcagagcatt acttgctctg ccgttttgct
      121 acgccttttc tgcggcagga gaagaagcac gtccggcaga acatgacgac acaaaaacac
      181 ctgcaattac ctcgacatct tctccttcat ttcgttttta cggcgaatta ggggttggtg
      241 gatatatgga tttagagggt gagaataaac ataaatacag cgacggtacc tatattgaag
      301 gtggcctgga gatgaagtac ggctcctggt tcggcctgat ttacggcgaa ggctggaccg
      361 tgcaggccga ccacgacggc aatgcctggg tgccagacca tagctggggt ggtttcgagg
      421 gcggaattaa ccgtttctat ggcggttatc gtaccaatga tggcaccgaa atcatgctca
      481 gtctgcgtca ggattcctcg ctggatgacc tgcaatggtg gggcgacttc acccccgatc
      541 tgggctacgt cattcccaat acccgcgaca ttatgaccgc gctgaaggtg cagaacttaa
      601 gcggcaactt tcgttatagc gtcaccgcca cacctgccgg acatcatgac gaaagcaaag
      661 cctggctaca ttttggcaag tacgatcgct atgacgacaa atacacctat ccggcaatga
      721 tgaacggtta catccagtat gaccttgccg aaggcatcac ctggatgaac ggtctggaaa
      781 tcaccgacgg cacaggacag ctctatctca cgggcctgct aactcctaac tttgccgctc
      841 gcgcctggca ccataccgga cgcgccgacg ggctggacgt accgggaagt gaaagtggga
      901 tgatggtgag cgccatgtat gaagcgttaa agggcgttta tctctccacc gcttacacct
      961 acgccaaaca tcgccctgac cacgctgacg atgaaaccac ctctttcatg cagtttggta
     1021 tctggtacga atacggcggc ggacgtttcg ccacggcttt tgatagccgc ttctacatga
     1081 aaaatgcctc tcacgatccc agcgaccaaa tcttcctgat gcaatatttc tactggtaat
     1141 aaggactgta atttcatgaa aataaaaact attttaacgc cagtaacctg cgctctgctg
     1201 ataagttttt ccgcccatgc cgctaacgcc gacaattata aaaacgtgat taaccgtact
     1261 ggcgcgccgc agtacatgaa ggattacgat tacgacgatc accagcgttt taatccgttt
     1321 ttcgatctcg gagcctggca tggtcatctg ttgccagacg gccctaacac catgggcggc
     1381 tttccgggcg ttgcgctgct gacggaagag tacatcaact ttatggccag caatttcgac
     1441 cgcctgaccg tctggcagga cggcaagaaa gtcgacttta cgctggaggc atacagtatt
     1501 cccggtgcgc tggtgcaaaa actgacagca aaagatgtgc aagtcgaaat gactctgcgc
     1561 ttcgccacgc cgcgcacgtc actactggaa accaaaatca ccagcaataa accgctggat
     1621 ctggtgtggg acggcgaact gctggaaaaa ctggaagcga aagaagggaa accgctttcc
     1681 gataaaacca ttgctggcga ataccctgac tatcagcgca aaatcagcgc cacccgtgat
     1741 ggcctgaaag tcacctttgg caaagtgcgc gccacctggg atctgctgac ctccggcgaa
     1801 tcggaatatc aggtgcataa atccctgccg gtgcagactg aaatcaacgg taatcgcttt
     1861 accagtaagg cgcatatcaa cggttcgacc acgctctaca ccacctattc ccatctgctg
     1921 accgctcagg aagttagcaa agagcaaatg cagatccgcg atattctggc gcgtccggcg
     1981 ttttatctca ccgcctcgca gcaacgctgg gaagaatacc tgaagaaagg gttaaccaat
     2041 ccggatgcga cgccggaaca gacgcgcgtc gcggtgaaag cgatcgaaac gctcaacggt
     2101 aactggcgct caccgggcgg tgcggtgaaa tttaacaccg ttacaccgtc ggtgaccggg
     2161 cgctggttct ccggcaatca gacctggccg tgggatacct ggaagcaggc gtttgcgatg
     2221 gcgcatttca atccggacat cgccaaagag aatatccgcg cggtcttctc ctggcagatc
     2281 cagcctggcg atagcgtgcg tccgcaggat gtgggctttg tccccgacct gatagcgtgg
     2341 aaccttagcc ccgagcgtgg tggcgatggc ggcaactgga acgaacgtaa taccaaaccc
     2401 agccttgccg cctggtcggt gatggaagtg tacaacgtca cccaggataa agcctggttg
     2461 gcagagatgt acccgaaact ggtggcctat cacgactggt ggttacgtaa ccgcgatcac
     2521 aacggcaacg gcgtgccgga atatggcgcg acccgcgaca aagcccacaa cactgagagc
     2581 ggcgagatgc tgtttacggt gaagaaaggc aacaaagaag agacgcagtc tgggctgaac
     2641 aactacgccc gcgtggtgga gaaaggccag tacgacagtc tggaaattcc ggcacaggtc
     2701 gctgcgtcgt gggaatcggg gcgtgatgat gccgccgtct ttggttttat cgacaaagaa
     2761 cagttggata aatatgtcgc taatggcggc aaacgtagcg actggacggt gaaattcgcc
     2821 gaaaaccgca gtcaggacgg aacgttgctg ggctactcgc tattgcagga gtcggtggat
     2881 caggccagct atatgtacag cgataaccat tatctggcgg agatggcaac catcctcggt
     2941 aagccagaag aggccaaacg ctatcgccag ttggcacagc agctcgcgga ctacatcaac
     3001 acctgtatgt tcgacccgac tacccagttc tactatgacg tacgtattga agataaacca
     3061 ctggcgaacg gctgcgcggg caaaccgatt gttgagcgcg gtaaagggcc ggaaggctgg
     3121 tcgccgctgt ttaacggtgc ggcaacgcag gccaatgccg acgcggtggt gaaggtgatg
     3181 ctcgatccta aagagttcaa cacctttgtc ccgctgggaa cggcggcgtt aaccaatccg
     3241 gcttttggcg ctgatatcta ctggcgtggg cgcgtatggg tggatcagtt ctggtttggt
     3301 ctgaaaggga tggagcgtta cggttatcgc gatgatgccc tgaaactggc ggatacgttc
     3361 ttccgtcacg ccaaaggatt aaccgccgat ggcccaattc aggagaatta caacccgctg
     3421 actggcgcac agcaaggcgc accaaatttc tcctggagtg ccgcgcattt gtatatgttg
     3481 tataacgatt ttttccgtaa gcaataattt gcaactgctg gcggatgcgg cgtaaacgcc
     3541 ttatccgccc tacatgtgca atcctcctgg ccggataaaa cgcgacaagc gtcgcatccg
     3601 gcatctgcac cgtgccactg gcggatgcgg cgtgaacgcc ttatccgccc tacatgtgca
     3661 atcccccagg ccggataaaa cgcggcaagc gtcgcatccg gcgttaccac cgggcgtatt
     3721 ctttttgaat cccatcacaa accccgcact ccccttttcc cttttctccg gcgacggcta
     3781 aattagaact catccgacca cataacaatt attttacata ctggacaatt ttatgagcta
     3841 cccgtcgctg ttcgccccgc tggatttagg ttttaccacg ttaaaaaacc gcgtgttgat
     3901 gggctcaatg cacaccgggc tggaggaata cccggacggt gccgagcggc tggcagcgtt
     3961 ttatgccgaa cgcgcccgtc acggcgtggc gctgattgtc agcggcggca ttgcaccaga
     4021 tttaacaggc gttggcatgg aaggtggcgc aatgctcaac gacgccagcc agatcccaca
     4081 ccatcgcacc attaccgaag cggtgcatca ggaaggcggc aaaatagccc tgcaaatttt
     4141 gcataccggg cgctacagct accaaccgca tctggtcgct ccctccgcat tgcaggcccc
     4201 catcaaccgt ttcgttcctc acgaactcac ccatgaagag atcctgcaac tgatcgacga
     4261 tttcgcccac tgcgcgcaac tggcacggga ggcaggatac gacggtgtag aggtaatggg
     4321 gtccgaaggg tatttgatca acgaatttct gacgctgcgc accaatcagc gtagtgacca
     4381 gtggggcggc gattaccgca accggatgcg atttgccgta gaagtagtgc gtgcggtgcg
     4441 cgaacgcgtc ggcaacgact tcattattat ctaccgactg tcgatgctcg acctggtcga
     4501 agacggcggg acttttgccg aaacggtaga gctggcgcag gccattgaag cggcgggcgc
     4561 gaccattatc aacaccggca ttggctggca tgaagcacgt attccgacca ttgccacgcc
     4621 cgtgccgcgc ggcgcattta cttgggtcac gcgcaaactg aaaggccacg tttcgctgcc
     4681 gctggtgacc accaaccgga ttaacgatcc gcaggttgcc gacgacattc tctcgcgcgg
     4741 cgatgccgat atggtgtcga tggcacgacc gtttcttgct gatgcggaac tgctgtcaaa
     4801 agcgcaatcg gggcgagccg atgagatcaa cacctgcatt ggctgcaatc aggcctgcct
     4861 cgatcagatc ttcgttggca aagtcacctc gtgcctggtg aatcctcgcg cctgtcacga
     4921 aaccaaaatg ccaatccttc ctgccgtgca gaaaaaaaat ctggcggtgg tcggtgcggg
     4981 acctgctggg ctggcgtttg ccattaacgc ggcggcgcgt gggcatcagg taacattgtt
     5041 tgatgcccac agcgagatcg gcgggcagtt taatatcgcc aaacagatcc ccggcaaaga
     5101 ggagttttac gaaacgctgc gctattacca ccggatgatc gaagtgacgg gcgtgacgct
     5161 gaaactcaat cacaccgtga cggcggatca gttacaggcg ttcgatgaaa cgatcctcgc
     5221 cagtgggatc gtgccgcgcg ttccgcctat cgacgggatc gatcatccga aggtattgag
     5281 ttatctcgat gtactgcgcg acaaagcgcc ggtaggcaac aaggttgcca tcatcggttg
     5341 tggcgggatt ggttttgata cggcgatgta tttaagtcag ccgggcgaat ccaccagcca
     5401 gaatatcgcc aggttctgta atgagtgggg gatcgacagc agcctacaac aggctggtgg
     5461 cttaagcccg cagggaatgc agatcccgcg tagcccacgg caaattgtga tgctccagcg
     5521 caaagccagc aaaccgggac aggggttagg caaaaccacc ggctggatcc atcgcaccac
     5581 cctgctctcg cggggtgtga aaatgatccc aggcgtaagt tatcagaaga ttgacgatga
     5641 cgggctgcat gtggtgatca acggcgaaac gcaggtatta gcagtggaca atgtggtgat
     5701 ctgcgcaggg caagagccaa accgcgcgct ggcgcaaccg ctgattgata gcgggaaaac
     5761 tgtgcattta attggcggct gcgatgtggc tatggagctg gacgcacgac gggcaattgc
     5821 ccagggaaca cggctggcgc tggagattta aatcgttatt tgaagcgccg gatgcaacgc
     5881 atccg
//
LOCUS       DAZKIV010000210         5873 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZKIV010000210 DAZKIV010000000
VERSION     DAZKIV010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.212"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..929
                     /locus_tag="ACHOT6_004378"
     CDS             <1..929
                     /locus_tag="ACHOT6_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFP9949481.1"
                     /translation="PAAPAPEAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNK
                     LQIPVQSSLARYADWLKDFKQGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPAL
                     QPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPV
                     AESYHAAIQEINDKRQLAELKNIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDA
                     AMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQ
                     LQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(979..2727)
                     /locus_tag="ACHOT6_004379"
     CDS             complement(979..2727)
                     /locus_tag="ACHOT6_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFP9949482.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEDKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEII
                     AGLTGYEAFMELLHKG"
     gene            complement(2727..3797)
                     /locus_tag="ACHOT6_004380"
     CDS             complement(2727..3797)
                     /locus_tag="ACHOT6_004380"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFP9949483.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAAFKNCATQ
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            complement(3787..5238)
                     /locus_tag="ACHOT6_004381"
     CDS             complement(3787..5238)
                     /locus_tag="ACHOT6_004381"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFP9949484.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIVSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(5249..5695)
                     /locus_tag="ACHOT6_004382"
     CDS             complement(5249..5695)
                     /locus_tag="ACHOT6_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFP9949485.1"
                     /translation="MATLTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
BASE COUNT         1529 a         1489 c         1494 g         1361 t
ORIGIN      
        1 aacctgccgc accagctcct gaagctcccg ttgcaaaagc agaagctaaa cctgaaacac
       61 ctgcgcaacc ggtggtcgat gaacaagcgg ttttcgacga aaaaatggat gtctatatca
      121 agtgctataa caagttacag atccctgtac agagcagtct ggcgcgttat gctgactggc
      181 tgaaagattt taaacagggg cctaccggtg aagagcgtac tgtttatggc atctacggca
      241 ttagtgaatc caacctcgct gagtgtgaaa aaggcgtaaa aagtgctgtg gcgttaacgc
      301 ctgcgctgca accaattgat ggcgtggcgg tgagttatat cgatgctgcc gtggcgttgg
      361 gtaataccat taacgaaatg gataaatatt acacccagga aaattataaa gacgatgcgt
      421 ttgcgaaagg gaaaacgctg caccagacat tcttaaaaaa tctggaagcc tttgaacctg
      481 tagcagaatc ttatcatgcg gcgattcagg aaattaacga taagcgccag cttgcggaac
      541 tgaaaaatat tgaagaaaga gaagggaaaa cattccacta ctactctctg gcagtcatga
      601 tttcagcgaa acaaattaat aacctgatat cgcaagagaa gtttgatgtt gatgcggcaa
      661 tgaagaaagt gtctgaactg gaaacgctag tggcgcaggc caaagaagcg gataaaggcg
      721 gcatgaattt ctcgtttatt aattcggcag gccagtatca actcgaggct aaaaaatacg
      781 ttcgccgcat cagagataaa gtcccgtact ctgactggga taaagagcaa cttcaggacg
      841 caaactcaag ctggatggtc gaagactctt tcccgagagc attacgcgag tacaacgaaa
      901 tggttgatga ctataatagc ctgcgttaaa cgtttttgat gataaatgca gtgtcggatg
      961 cggcgcatcc gacacagttc accccttgtg aagtaactcc ataaatgcct catatcctgt
     1021 taacccggca ataatctccg ccggatgctg acacaatatg cttgccagat agctaaatat
     1081 ttttagctca tgacgcgcat cggcggcggc gcaggcgatc aagacatggt taatgacttc
     1141 gttattcacc tcaactggtt gggcgagggt aataaaaaat ccacgaaaac gtcgctcctg
     1201 ttcgctccag caatgtggga tggcgaggcg attaacaatc aggttttcac cttcgccttc
     1261 gcgggcgatg atgcgttgtg cttcatcggc ggtaatatgg tgttgtgcga ctaattgcgc
     1321 acaaatttgc cgggtaatat gttgccagct ttcaccgcgt acattcgaat aatgaaaact
     1381 tccgggggca gagaaaaaac gctccggctg ttggcgaata aatgccgtcg ccagaaaatg
     1441 ttttatttgt tcgataccgg cagccgtaat gatatttttt acggtgatgt aattatttac
     1501 cgcgtcatcc agtaaataat ggctgttgtt aatgatcaat aacggctcaa tctcttcgcg
     1561 aatggcaaca agttcgctta agctacgggc aataatcacc cgacaatgta aaacatcgcg
     1621 ctcaatggcg agctgattaa tagtggcaat cgcgttctgg tccgagagca aaatgatcgg
     1681 ctggcgttcg ttttgatgcc gttccagcgc acaggcaaaa tacaaaccga tcagatcgct
     1741 gtcgaaaagg ggaatatcga gctgttcacg cagtagcgta ataaagtgca gactcatatc
     1801 aaaggccgca ggccaggctg ctttcaggtt attcatgctg ctttggcggt gctcagcaat
     1861 ccacactggt gcggcaaggc aacgtaataa atgcccggta atgttctcaa ccagctgcgt
     1921 atcaataata ttcaatgacg cgatgcccgg caccctttgc agcaaccgct gcacattatc
     1981 ggcagaaatt atctgcgctt gctgatgctg tttctctatc aaacctgtca gggttcttac
     2041 agcgttatca ccaagaaaca tgccgctttg ttcaacgatt tgttttatct cgtcaccggg
     2101 atattgcggc cactcatcgg tcagttgatt acgcagggcg taaatcgcca gaatcaggct
     2161 ggagagataa tcaccctgca tgagcggcca gcggtgttgg ttgtcgcagg ccgtggataa
     2221 atgctgaagg ttgtctcgtg ttacgcccgc cagcggaatt aaaaacggat ctttgcgcag
     2281 caagttcgcc agcaagataa cgcgcttatc ttctgtctca tcaatgaaat ggcccaaacc
     2341 ggggcggctg gccaggcaac aagcgcgttc ataacgctgt tttaaccggg gcagacgctc
     2401 tgcgacccac gtttctggca aattaagcgc cgaggcgagt tgcgcacggg gagtaaaggt
     2461 attcagtaat aacagtgcca gcagccgatc gtcgttatcg tgcttttgca gcaactgaaa
     2521 aaagctgcgg cgctcgaaga tttccagctg atagcccgca ctaccactgg cggaaatgcg
     2581 ggctttgccg ttaagggtaa agttgagata gtcaatatca cgcaggatgg tcctgcctga
     2641 aacgccagtc tgttgcgcca gctcaccagg cgtgcgcggc tgttgctcca gcagatcgac
     2701 aatctttaac tggcgttcgt ttagcattaa tccacctgac ggaactggct aaacgcgttg
     2761 actttctccg cagttaaggt cgtcagaaaa ccccgtatca gcgtgagcag agcatcgtaa
     2821 tcggcttttg aaatcatacc gctgttggcg tgcagataac gagttggcag acacagcgcg
     2881 acaaccggac gcccgccgcc catcacgttg tagcgcccgc catccgtcgc tccggttttc
     2941 atggtggaaa attgcagcgg taaatcattc tgcgtggcac agtttttaaa cgctgctacc
     3001 agtttctggt tggggaagta gcgcttgtca aacagcatca gccccggccc ctggcccagt
     3061 ttcagcgggt atttaatgtt atcaatgccc ggaacatcac ctgcgacagc ggtatccagc
     3121 acaatcacca catccggttt aatgtgttcc gccgaggttt gtgccccgcg tagcccaact
     3181 tcttcttcca cgctgccgac gccatacagc gtaatttcgg gattattcac cgtctgcaat
     3241 agttcagcca tcattgcgca gccgatgcgg ttatccagcg ccttgccgac cactttatct
     3301 tcgccccagc aggcaaaatt ggcttccggg ctaataaaat cgcccatcgc cacaccgcgt
     3361 ttttccacct catcgcgact gttcgcgcca atatcaatga acatctcatc aaatgacagc
     3421 ggctgttgct tttgcttttc cgttaacgca tgaggtgcga cggaaccaat cacaccaggg
     3481 attttaaatc ccttgtgtgt gcgtatggtt acccggtggt tgagcattga ctgattccac
     3541 cagccgccaa tggtggtaaa gcgcagaaaa ccgctctcgt cgatgtgggt gaccataaag
     3601 ccgacttcat ccatatgccc gacaacggca acttttggac ctttattccc cttacgggca
     3661 acaaagctgc ccagaccatc aaaggtgatc tcattaacgc aaggttccag cgtgtttatc
     3721 agaatgtcgc gaacttcctg ttcatcgccg ctgacggcgc tggcttcgca caactgttgc
     3781 agtaactcaa tgttcatgga caagctcctg ttgcgcggct ttcgctttgc ggcgtaacca
     3841 caatcctttc aggacgataa tgaatgaaat gttcagcgcg aggccaatcg ccagcaccag
     3901 ataaaacgaa cccaccggcg acatcaggcc gatcagcggg tcaaagatac ccaaaccagg
     3961 tgccagacgt ttgattccga aagcgatcac cagcacgccg gtaatgccgc ccgccagcgt
     4021 attggcggta atcatcggca acggagctgc cagagcgtaa ggtatagccg gttcggttgc
     4081 gactgtggca ccgaccacaa tggcactact ggcagcctct ttttcctgtc gggtaaacag
     4141 tttgggcgca atgaacgtcg caagacccgc cgcaacgggc ggcatcaacg cgacaacgcc
     4201 gacgatggcg taccagtcat aaatgtgttt ttccagcagt gagaagcaga agaaccaggc
     4261 ggttttattg atggggccgc ccatatcgaa cgccagcatc gcaccgacca gaaacgctgc
     4321 cgcaaacttc attgatggag gaatggtatt caggaagtgg agcagcccgc ccatgaggtc
     4381 ggacatcacc ggcccgataa cgtagtaagt gagcacgccg aaaaccagca gagtaacgaa
     4441 cgggatcagc attgaaccga gcagcggttg taatgctttg ccaaggcgca ctttgcggaa
     4501 ccagaagacg aaatagccaa tcgccagccc cagcaccacc gcgccgagga atcccgcacc
     4561 cgactgggtg ccgagcagcg ctttgtcgtt cgccagatag caaaccagaa acgccggagc
     4621 gaaagccggt ttatcggcaa tcgacgaggc gatataagcg cccatgatgg ggatcataaa
     4681 ggtgaagccg aggtaaccga tggattcaac tacccaggta aacgacggcg cacctttcga
     4741 catatcggta tacggcaaac cgaactgcac cagcatgttg gcaagcgcca ccagaatacc
     4801 gccgccgatg acaaacggca gcgccgcaga gacgcccgcc atcaggtggc tcatcacgct
     4861 gccgctttgc acttcctgtt tgccaagctt cacgccgcta tctgcagcaa aaagctgcga
     4921 gtttgttggt aattcactga caatctgatc gatatttttc agcgcctggg agatggcaat
     4981 ctcataaacc ttcttcccgg caaaccgcgc gcgatcgtca ccgctcaggc cacgcccggt
     5041 agcgagaatg acgtaatcgg cagcggcaat ctcttcgctg gataagcggt tttcgacgcc
     5101 gcttgatcct tgagtttcaa ctttaatggt atgaccgaga gaacgggctt tctgttccag
     5161 ggcttccgcc accatgtagg tgtgagcgat cccggcgggg cagttggtga tcgcgacaat
     5221 acgtaaggat gactccatcg ccacttcctt atgagctgag ggtttgattt aacaaggcaa
     5281 gcacctggtc ggcgtcgccc tgttgcagtt gatgaataaa atcctggtgg ataattttgc
     5341 gacacagtgt gccgatgatt ttgacctgat cctcttcgcc gctttgcggc acgccgaggc
     5401 agatccagca attgacgtct tcgccatcgc tggctttcca gtcaatagcc tgcgctttgc
     5461 gggcgaataa tacgaacggt tgtttaacgc aggcgctttt accgtgcggc acggcgacac
     5521 cggaaccaaa tccagtagag tgcatttttt cgcgcagcag cagggtttgc aggaactggt
     5581 gtgagtcggt gataaaaccg ttttgtaacg ctattgtcgc caactgcttc agaacggaat
     5641 aagcgccatt gccctgaata ttcaggtcaa tacagcttgc agtaagagtt gccattaatc
     5701 ggactccata aaatatattt taatcaattg taatggtgga gcgattaagt gatgagaggc
     5761 gatatgtcgg taaacaatga caatttgaga cagagtgaaa ggtcagattt gcggagtaat
     5821 gcacataatg gttatttaaa taaaccacat gaatcattaa tgggttattc att
//
LOCUS       DAZKIV010000211         5592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZKIV010000211 DAZKIV010000000
VERSION     DAZKIV010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.213"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..998)
                     /locus_tag="ACHOT6_004383"
     CDS             complement(<1..998)
                     /locus_tag="ACHOT6_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFP9949486.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDAGGASENSTRWG
                     VDKPLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRAD
                     LADMAGQCVGGSADGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMT"
     gene            1239..1373
                     /locus_tag="ACHOT6_004384"
                     /pseudo
     CDS             1239..1373
                     /locus_tag="ACHOT6_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309987.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1478..1548)
                     /locus_tag="ACHOT6_004385"
     tRNA            complement(1478..1548)
                     /locus_tag="ACHOT6_004385"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1518..1520),aa:Met,seq:cat)
     gene            complement(1712..2269)
                     /locus_tag="ACHOT6_004386"
                     /pseudo
     CDS             complement(1712..2269)
                     /locus_tag="ACHOT6_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211421.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
     gene            complement(2540..3094)
                     /locus_tag="ACHOT6_004387"
     CDS             complement(2540..3094)
                     /locus_tag="ACHOT6_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9949487.1"
                     /translation="MIYPTNTGKSGERLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGKQKSWLVHCTDAEAL
                     CIDRVISEVLAEHPGLICILRQRYEGRGMTKRKMAELLNDSHPEWCFRTCCSRVDVWL
                     NLAEYMLYLPMRDAFSSGDLKTVC"
     gene            complement(3091..3381)
                     /locus_tag="ACHOT6_004388"
     CDS             complement(3091..3381)
                     /locus_tag="ACHOT6_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HFP9949488.1"
                     /translation="MVDLRKAAKGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(3381..3980)
                     /locus_tag="ACHOT6_004389"
     CDS             complement(3381..3980)
                     /locus_tag="ACHOT6_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HFP9949489.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHMPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            complement(4136..4213)
                     /locus_tag="ACHOT6_004390"
                     /pseudo
     CDS             complement(4136..4213)
                     /locus_tag="ACHOT6_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4442..4927)
                     /locus_tag="ACHOT6_004391"
     CDS             complement(4442..4927)
                     /locus_tag="ACHOT6_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HFP9949490.1"
                     /translation="MAEIVEGVHYLTVDDLVEINRSLIELQTPDELVGVLSPDNLSSS
                     QARPSMVRWYEQTNDMFVLASVLIESLIQNHPFANANKRTAMMAGYVFLLLNGYELTA
                     PGDEIVEMAEGLACKTYTREDLENWLCYWSRAYDSRELCKTGATIVLYETIKFKMEQQ
                     N"
     gene            complement(4946..5125)
                     /locus_tag="ACHOT6_004392"
     CDS             complement(4946..5125)
                     /locus_tag="ACHOT6_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949491.1"
                     /translation="MSYALKKHPRLTIPPRDKSVVAAPRPAIDENCTHREQVKNAFDF
                     GFSRYEKAMEELSKV"
     gene            complement(5334..5489)
                     /gene="hokD"
                     /locus_tag="ACHOT6_004393"
     CDS             complement(5334..5489)
                     /gene="hokD"
                     /locus_tag="ACHOT6_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9949492.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
BASE COUNT         1421 a         1511 c         1230 g         1430 t
ORIGIN      
        1 gtcatgaacg gtacgaaatg gatattcttt tccgttttgt ttttgtagct gccgtacacc
       61 gtctggtacg aggattcgtt cttctgcttc cagaaatacg tcgtgtcccc gcatatccag
      121 ggaacaccgt cagcagagcc accgacgcac tgacctgcca tatccgccag gtctgcacgg
      181 aatttatcaa ccagcgcacc aaactgtgct gcgtgatttg ccggcgtacc gccaaaatca
      241 aattccccct gcatccacac cacggcaaac agcacatttt tcggattctt cttcagtgct
      301 gcttttgttc gaccgataag gtccttatac agcggcttgt ccacacccca gcgggttgaa
      361 ttctccgagg caccgccagc gtcactgtat gtgccatcgg ctccggtggt gaacgctgaa
      421 ccaccacgac agcacggaac cagcagaatg cccgcattcg ccggtataaa cggcagcagt
      481 tttttggcaa tatgcagccc ctgccccacg gtaccgtact gcccctttga caggtccgct
      541 ttcggatggt taaggcggct catgtcctgc acatcatgca gacaatggtc cgccggaatg
      601 atgtcgttat acttacaggc ggcaccgccc ggtgtcaccg tactgcgacg cgccagctgc
      661 ttaatgcgcg ggtccggacg gtcatatgtc tccggcagcg gaaggccttc accatatgcc
      721 atgctgtttg actgccccgc cagaaccaca acaaagtaat actccgggtc tctggtggcg
      781 ctgattactg tgccctctcc atccgacggc ttcaccacaa caggtgtgct cacatcacct
      841 tctgcgacaa tcgcctgaat aagtgctgcg ccatcatccg tatacgaaga aaacggcccg
      901 ccgtatggct gccatccttc acgaattttt tgagcaagcg catccgcaag gtctgacggc
      961 gacgccgccc tgacaacatc ataatgttta aatgtcatta ttcctcccgg ccgggatagt
     1021 gtattaaatc agatatggag tgggctgtag tccggaagcc tgaatgacac acggggacta
     1081 cagcccaaga aatgaaaaaa ggccacgcag ttgcgcagcc tgataaaccc tggttaaaat
     1141 ccacacgata acaacacaac aatatcagta tctcatgcta ttgcccgaac ccattcgggc
     1201 attttttacc cataaaaaat gcccctccgg agaggggcat gtttgcatgc acattctttt
     1261 tcttgcatgg tgccgggtgc ctcccggtga attcagtacc agcacctgaa tccgcgatta
     1321 tcccatatac ctactcactg attgcccctc cgcacagggg gattcaccat gccagtttct
     1381 tttaacaaac tccccgcaaa ccagacaaca gtcaaccgcc tgaattgtga gacatttaaa
     1441 aaaaaggccc gcaaaagcga gccagggaaa ataagtgtgg cgcgttgtac tggattcgaa
     1501 ccagcgacct ggcgattatg cgtcgctcgc tctcaccact gagctaaagg gccgggagca
     1561 gaataataac ggtccgtaat taattccgca ataaaaaacc cgctcaatgg cgggttctgg
     1621 taaagttcat gcgcttggtt cgcctcgcga tacagctttg cgaagcgtac cggaattgaa
     1681 gcagtttatg tgcaaaaatg caagaacttt tttaaagctg catcaacctt tccaccagtt
     1741 tatctctgcg aacaacaaac caaccattgg ctctcgccag ttccagccat gactcaaggg
     1801 aaataacaat atcatcatcc cgtaactgaa ttgtggaaac agtgacacca cctcactgat
     1861 aacagagaac tcgcgtgtcg taacttttct ggcatgcaac tggcgctgac ggatcctttt
     1921 gactgaaata gcagtcttcc agtttttcga acacatccca cgcctgatcg gtttcgagca
     1981 tttttgcgtg gcgggctgct ccgcgttctg tccagaggat aagggagcgg gctttgggag
     2041 agatgggatt ttgtgagtag tttaaagcta cccgcaattc tttaaggtca ttaccaacaa
     2101 ctttgaaaaa gtgtttccct tcaacgaagc gtactttgtt ctcatgatga ttctggcgaa
     2161 tacgcaccgg ctcagtgccg taaagctgcg ccaaaagttc ggtggtaata acaggaatcc
     2221 ggttatgggt gatcggggag agagtttcaa cagaaatttg agttgtcata atgacgccct
     2281 ctggtggttt ctaaactatc accaccatca ggttccaatc atcgggtggt gagacgcaca
     2341 gggttggaac taccgggaaa ccgaccggcg agcttttcag ctcccccatg cgccccacca
     2401 taattcagat gtgcgcgtgc atacgacaat aaaaaacacg ctcgcggcgt gtgtctgtcg
     2461 cggtctctat ccggggttcc aatcccgacg ccagattttg ctggcgcgtg aggaatatag
     2521 ccccggataa cagattgagt caacagacgg tttttagatc cccggaagag aatgcatcac
     2581 gcatcggtag atagagcata tattcagcaa gatttagcca tacatctacc cgactgcagc
     2641 acgtccgaaa acaccactca gggtgtgaat cattcagcaa ttcagccatt tttcgcttag
     2701 tcatccccct cccttcatat cgttgccgga ggatacaaat caatcctggg tgttctgcca
     2761 gcacttcact aatcacccga tcaatgcata acgcctctgc atcagtacaa tgcaccagcc
     2821 agcttttttg cttgccgttg atcatatccc gcaaaaaagc ctcaagttca ggtttgtcca
     2881 aacctgcttt tttcatcctc cggagcgcct cgttaattgc cgtttttgtc agctttttag
     2941 aggtcagcaa ctggttgaac atatttcccg tcttaccgcc gccaatatac gaccagcgcc
     3001 cccacatgcg cagttttccc tgaatccaga cactttccag cgtggtgaga cgaaggcgtt
     3061 ccccgctttt gcctgtattt gttgggtaaa tcataaataa ccttcctttc tccagatttc
     3121 ttgtgtgcga aaaacacctt ctgcatgcat caggcgcaat tcttctttgg tgtaatcgct
     3181 ggtttttacc cgcccgtcga ttaaatcgtg gcatgagcta caggcaattg ccgcctgcat
     3241 atcgtgtggt tttgtcgctg ttccacacgt ccccgccagc ctgtaatgcg ccagcacgga
     3301 cgtttcggga ttgtgattgc agtagccagg aattctgact gtacacatct gaccttttgc
     3361 cgctttacgt aaatccacca ttacgcaaac tccagcagct gcgcggccac attttcgact
     3421 tcctccggag aggaaaattt acggaacagg atccagttcc acagcacatt cagtacagat
     3481 ttataaacct gctgaaactc ggtttcgtcc atgttcgcaa atgcgataga ttttgccctg
     3541 cgcccacgac taccatcagg ataaatatgc tcggtgtaaa atcctgcctg aatggttacc
     3601 cactcgcgga aagcctcaaa cgactttagc aatgctgtat ctcgggttct gcgtgtcgca
     3661 actgtattca gatattgctc tgcggcttcg ctcagagctg gcatatgttc ccggcctact
     3721 gattcgcaca gataatcaac gaaaccggac accagttttc gctcgcgagg agtgatcgcc
     3781 ccaccgaccg gagtccagta atcgaatccg agttgcagaa gtttgaaaaa acgcttatgg
     3841 aatgcgtagt tacgaacgcg cttgaaatca gcgtgtatcc actcgcctat tttgatttga
     3901 tgcagaaaat cacaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg
     3961 accagttgta tatgcgccat tgctgtctcc aatggcgctg taggttgcca gttgttcagg
     4021 ctggcttacg aattataact cattcgcgaa ctaccttgaa accgagcctt tcaaggtatt
     4081 caatgaatgc ctcgatagat aaaattacat gatcatcagg gattaacgtt gtgtagacaa
     4141 ctttcccatt ctcaacgcgc acagcataga ggccattttc actaaaaatt tcacgcaatt
     4201 cttcgatttt catcttcaga atccttccag ataaatagct cttccccttc ggggtccatc
     4261 cctcttctcc ctgcgcgcta cttaagagcc tcgattctag ccgtgaagac ataccgatca
     4321 acagcaataa gccacctgaa taacagaatc agccaaccac atcagtacaa ccagatgcat
     4381 catactcacc gaaaataaac aacaaaaaac ccgccgaagc gggttttcat tggaagcacc
     4441 tttagttttg ctgttccatt ttaaacttga tagtttcata caaaacaata gttgcgcctg
     4501 ttttacataa ttcccggctg tcatacgcgc gagaccaata acacaaccag ttctcgagat
     4561 cttctcgagt ataggttttg caggccagtc cctctgccat ttccacgatt tcatcgcctg
     4621 gtgctgttaa ttcatagcca ttcaacaaca agaagacgta accagccatc atagctgttc
     4681 gtttgttcgc attagcaaac ggatgattct gaatcagact ttcaatcaat accgatgcca
     4741 gtacaaacat gtcattagtc tgttcatacc atcgaaccat gctgggacgg gcctgagaag
     4801 aacttaagtt atctggactc agaacaccaa cgagctcatc tggcgtctgt aattcaatta
     4861 gggaacgatt gatttcaaca agatcatcaa ccgtaaggta atgcactcct tcaacaatct
     4921 cagccataga gcacaatacc catcattaca cttttgaaag ttcttccatt gccttctcgt
     4981 aacgagaaaa accgaaatcg aaagcatttt tcacctgctc acgatgagtg caattttcat
     5041 cgatagccgg gcgcggagct gccacaacgc ttttatcgcg agggggaata gtcaatcgcg
     5101 ggtgtttttt tagtgcgtag ctcattgggt gcccccctta cttctccgtc ctgttcttta
     5161 acaaaaatca agtcaataca aaattagtca agctaatgtt gttagcatat cctaatacca
     5221 atcgtattca caaccacagt acgtgaaaaa aaaccgccga agtaggttaa gtgcgggtgc
     5281 gttgaggatg cctgacacat cagaggtggc gggggtttac cccgcctggt ctcttactcc
     5341 tcaggttcgt aagctgtgaa gacagcgacc tccgtctggc cggttcggat tcgtacctcg
     5401 cagaggtctt tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg
     5461 gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcgcgtaa
     5521 gaggctaacc tacatgtgca agtcatagat atggcctcag attaatgtta agcgtcttgc
     5581 cggacgcgta at
//
LOCUS       DAZKIV010000212         5555 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZKIV010000212 DAZKIV010000000
VERSION     DAZKIV010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.214"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            123..1481
                     /gene="mltD"
                     /locus_tag="ACHOT6_004394"
     CDS             123..1481
                     /gene="mltD"
                     /locus_tag="ACHOT6_004394"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFP9949493.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1530..2300)
                     /locus_tag="ACHOT6_004395"
     CDS             complement(1530..2300)
                     /locus_tag="ACHOT6_004395"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9949494.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAHMLDVVAQAAEVRQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRLIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLTLINEANLIVDNLITDKLPLEFSSWVVRMRTPEALVD
                     AIRIYQQSASTEVRTYFSLQTDGSFTSDIIMVEAHKAA"
     gene            complement(2378..3178)
                     /locus_tag="ACHOT6_004396"
     CDS             complement(2378..3178)
                     /locus_tag="ACHOT6_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFP9949495.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3419..4333
                     /gene="yafC"
                     /locus_tag="ACHOT6_004397"
     CDS             3419..4333
                     /gene="yafC"
                     /locus_tag="ACHOT6_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFP9949496.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGQRYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4330..5133)
                     /gene="dkgB"
                     /locus_tag="ACHOT6_004398"
     CDS             complement(4330..5133)
                     /gene="dkgB"
                     /locus_tag="ACHOT6_004398"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFP9949497.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVITALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(5296..5372)
                     /locus_tag="ACHOT6_004399"
     tRNA            complement(5296..5372)
                     /locus_tag="ACHOT6_004399"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5336..5338),aa:Asp,seq:gtc)
BASE COUNT         1446 a         1349 c         1379 g         1381 t
ORIGIN      
        1 caacctgaag agcgttttgc atggttaagg tcaaagaaag ataggttctg ataaaacttt
       61 cttgtcatcg gctccgttcg ccgttatgat cggtcgtctt ttaagcaact attgacacac
      121 acatgaaggc aaaagcgata ttactcgcct ctgtcctgct cgtgggttgc cagagtaccg
      181 gcaacgttca acagcacgca cagagccttt ctgcagctgg tcaaggggaa gcagcaaagt
      241 ttacaagtca ggcacgatgg atggacgatg ggacgtctat cgcgccagat ggtgacttgt
      301 gggctttcat tggcgacgag ctaaagatgg gaattccgga aaatgaccgg attcgcgaac
      361 agaaacagaa atatttacgc aataagagct atctccacga tgtaacttta cgggcagagc
      421 cgtatatgta ctggatagca gggcaagtta aaaaacgtaa catgcctatg gaactggtac
      481 tactacccat agtggagagc gcttttgatc ctcacgcaac gtctggcgcc aatgccgcag
      541 gcatctggca gatcattccg agcacggggc gcaattatgg tttgaaacag acccgcaatt
      601 atgacgcgcg tcgcgatgtt gttgcttcaa caactgccgc gctgaatatg atgcagcgtc
      661 tgaacaaaat gtttgacggc gactggcttc tgaccgtagc ggcttataac agcggcgaag
      721 gtcgggtcat gaaggcaatt aaaacgaaca aagcgcgtgg gaaatccacg gacttctggt
      781 cgttaccgtt gccgcaggaa acgaagcagt acgtgcctaa aatgctggca ttgagtgata
      841 ttctcaaaaa cagcaagcgt tatggcgtac gtctgccaac gaccgatgaa agccgtgctc
      901 tggcgcgtgt gcacctgagt agcccggttg aaatggcgaa ggttgcagat atggcgggga
      961 tttccgtcag caagctgaag acattcaacg ctggcgtgaa aggctccacg ctgggcgcaa
     1021 gtgggccgca gtacgtgatg gtgccaaaga agcatgcaga tcaactgcgt gaatctctcg
     1081 cttcaggcga aattgctgct gtacagtcga cgctggttgc cgacaatacg ccgcttaaca
     1141 gccgtgttta caccgtacgc tctggcgaca cgctttcaag catcgcttca cgtctcggcg
     1201 taagcaccaa agatttgcag cagtggaaca aactgcgcgg atctaagctg aagccaggcc
     1261 aaagtttgac gattggtgca ggcagtagcg cacagcggtt ggcaaacaac agcgatagca
     1321 ttacgtatcg tgtgcgcaaa ggcgattcgc tttcaagcat tgctaaacgc cacggcgtga
     1381 acatcaaaga tgtgatgcgc tggaacagcg acactgcgaa tctgcaacca ggcgacaagc
     1441 tgacgttgtt tgtgaaaaac aacaacatgc cagattcctg acaaaccaga taataaaaaa
     1501 ggcaccgatt cccccggtgc cttttttatt tatgccgctt tatgtgcttc taccatgatg
     1561 atatcactgg tgaaagagcc atcagtctgc aaggaaaaat acgttctcac ctctgtcgat
     1621 gcgctctgtt ggtaaatgcg aatagcgtct actaacgctt ctggcgtacg cattctcacg
     1681 acccatgaag aaaattccag cggtaactta tctgtaatta aattatcaac tatcagattg
     1741 gcttcattga ttaacgtcaa ccactcaccg ctggcgtagt ttcgtacgtg agaggtatcg
     1801 cgtaatgctt ctaccgtctg taaccagatg tcgcgcactg ggtggcccgg agacattacg
     1861 tccatcacaa tcagcctacc gccaggtttc aatatccggt tcacttcacg cagtgctgca
     1921 ccaacatcat gccagtgatg ggcagaataa cggctgataa caatatcaaa tgcgttatcg
     1981 gcaaatggca gactttcggc ataaccctgg cgggtggtga tatttttcag ttgccggact
     2041 tcggcagctt gtgccacgac atccagcatg tgggcagata agtcatacgc caccaccgtg
     2101 ctcacgtttt gcgcagcgac aaagctggca tgccctgctc cgcagcccat atcaagtaca
     2161 cttgcaccag gataatcagc cagacgcacc gccaggcgct gtaaatctcg tccggatgca
     2221 tgtacggtac tggtcagata ttcgcaggct tgagagctaa actgcttttc tacgtggtca
     2281 tggtgggatt gtgttgtcat tgcgctgtcc ttttgttgtt tgagtataaa aggacagcgc
     2341 caaacgttgg tctgccctgt ggcagacctg acatacctta tttatcaggc ttgccgggac
     2401 tgaattcaac gagtagcgga ttgtgatcgg aagcgcgcgt aaccaataca gaagcttcgc
     2461 tgacgttcag accacggtag aaaacaaaat cgagcgggcg gccaaacgcc cgacggcgct
     2521 gatcatcggt aaaacgcacc tggcgcagcg acatttcccg cgcaaagcga tataacgcgt
     2581 tcatccttct acggctccag gcattgaaat ctcccgccat aatgaccggg ccgctgtggt
     2641 gagctatctg atcaccaata ggaagtaact gcttactata gacatccacg cccagactga
     2701 agttgacggc gtgtatatta accaccatca acaggcgggt gtcaggtaat ggatagaccg
     2761 tcaccagtgc cgacttcgcc agacgcaaaa tgggttctcg ttcacgtaac gggcagcaat
     2821 acactggatg cgccgccgaa agggtcatta caccggaagg atgttgtggc agcacgaaag
     2881 cgggcacctg atcggcggca agatagttag cggtcgcaaa ctgtactaac tctggcgttg
     2941 tctgcgcttc ctgcaataac accagatgtg catctttgcc gtagttcttt aataccgaca
     3001 accattcagc gcgttgctgt ttgtatatgt tccacaccag gatccgaata cgctcttcgg
     3061 tacttaacgg ttctccaggt ggtaatgcct ggccgatgct cgcaaaagac cccggcggta
     3121 agatcctttc cgcaggttgt ccggcaacat agcgcatggc ataggtgttt tttcgcactt
     3181 ttgactttga aacctctatt acgtcaaccc ttccgcactc gggagggttc tactgttata
     3241 gggattttag ctcactcttt caacgagcaa ttgtatattg ttatgtaagc aagtgcttac
     3301 acaggtcaga attttgctca ttattgagaa cattaaatcg gatgacatat cacgcatcct
     3361 gatggtttaa tgacaaataa attcaaaata tctcttctat tttgcaacag gagcaaaaat
     3421 gaaagccacg tcggaagaac tcgccatttt tgtttcggtc gtagaaagcg gcagctttag
     3481 ccgggcagcg gaacaattag ggcaagcaaa ctcagcggta agccgggcag tgaaaaagct
     3541 ggagatgaaa cttggcgtta gcctgcttaa tcggaccacg cgacaactta gcctgacgga
     3601 agaaggccag cgttattttc gtcgcgtaca gtcaattttg caggagatgg cagcggcaga
     3661 atcagaaatt atggagacgc gtaatacacc gcgtggactg ttgcgcatcg atgccgcaac
     3721 tccagtggtg ctgcactttc tgatgccgtt gattaagcct ttccgtgaac gctatccgga
     3781 agtcacttta tcgctagtct cctccgaaac gattattaat ttgatcgaaa gaaaagtgga
     3841 tgtcgcgata cgcgctggta cgttaacgga ttccagctta cgtgccaggc cgttatttaa
     3901 cagttatcga aaaattatcg cctcccccga ttatatttcc cgctacggga agccagaaac
     3961 gatcgacgat ttaaagcaac atatttgcct gggattcact gaacccgctt ccctcaatac
     4021 ctggccgata gcccgtagcg atggacaatt acatgaggtg aagtacggtt tgtcatccaa
     4081 tagtggggaa acactgaaac agctttgcct gagtggcaac gggattgcgt gtttgtccga
     4141 ctacatgatc gacaaagaaa tcgctcgcgg agaattggtg gagttaatgg cagataaagt
     4201 gttgccagtg gaaatgccat tcagtgccgt ctattacagc gaccgtgcgg taagtacgcg
     4261 catccgggct tttatcgatt tccttagcga gcatgtaaaa acagctcccg gaggagctgt
     4321 cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc ggtcgttgca
     4381 atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt gtgccttaag
     4441 attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac cttcccccat
     4501 agcccacgcc agaatcactt gtgccggagt cgcattgtgt ttagctgcga tacgagcaat
     4561 aacctcatct ttcagggcct taccatacgc cagcgtcata taggaagtaa tatggatgcc
     4621 gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga gttcaatctg
     4681 gttagtagcg atgttttcag caccaacagc agcaatcgct ttttccatca acgggatcgt
     4741 gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca gcagcgcctg
     4801 cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta gcgtcagatc
     4861 aacataatcg gtacgcaatt tttgcaggct ctctttcaga cttgggatca atttgtcttt
     4921 gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg gcacgccact
     4981 ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg tatcaattgc
     5041 gcgataacca agttcaagcg ccgttatcac agatgaaata acaacgtcgt ctttcagacg
     5101 gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat gctattttcg
     5161 ttaactgagg tgattatggg cttcagactg ttgaggaaaa agagcgtaaa atgcagagga
     5221 tttttgcgat tctggcaata atagatatac agacgaaaaa aaacctcgag cgttaactcg
     5281 aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc tgcgtgacag
     5341 gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac caccggtttt
     5401 atgaccggct tactgcttaa tttgatgcct ggcagtttat gaatcacttc gtgattcacc
     5461 cttcgggccg ttgcttcgca acgttcaaaa gctttcgctt ttgtcctact ctcttatcga
     5521 gtagtgaact gcctcaaagg tcagtgccat tcttc
//
LOCUS       DAZKIV010000213         5549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZKIV010000213 DAZKIV010000000
VERSION     DAZKIV010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.215"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..305)
                     /locus_tag="ACHOT6_004400"
     CDS             complement(<1..305)
                     /locus_tag="ACHOT6_004400"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFP9949498.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLL"
     gene            504..1202
                     /locus_tag="ACHOT6_004401"
     CDS             504..1202
                     /locus_tag="ACHOT6_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9949499.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(1247..2146)
                     /gene="eamA"
                     /locus_tag="ACHOT6_004402"
     CDS             complement(1247..2146)
                     /gene="eamA"
                     /locus_tag="ACHOT6_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFP9949500.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            2341..3528
                     /gene="ydeE"
                     /locus_tag="ACHOT6_004403"
     CDS             2341..3528
                     /gene="ydeE"
                     /locus_tag="ACHOT6_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFP9949501.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSHQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3655..3750
                     /gene="mgtS"
                     /locus_tag="ACHOT6_004404"
     CDS             3655..3750
                     /gene="mgtS"
                     /locus_tag="ACHOT6_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFP9949502.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            3750..3854
                     /gene="mgtT"
                     /locus_tag="ACHOT6_004405"
     CDS             3750..3854
                     /gene="mgtT"
                     /locus_tag="ACHOT6_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFP9949503.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(3969..4859)
                     /gene="dgcZ"
                     /locus_tag="ACHOT6_004406"
     CDS             complement(3969..4859)
                     /gene="dgcZ"
                     /locus_tag="ACHOT6_004406"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFP9949504.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(5114..5506)
                     /gene="ydeI"
                     /locus_tag="ACHOT6_004407"
     CDS             complement(5114..5506)
                     /gene="ydeI"
                     /locus_tag="ACHOT6_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFP9949505.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPSPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1515 a         1335 c         1164 g         1535 t
ORIGIN      
        1 tgcagtagtt tcatcgcaaa ttcaagaact gcctgtccgt tcatggtgcc gtagtctttc
       61 atctcgataa cggctacagg cattttcccc tgtgcaacgg cttcaatctc tggtttctga
      121 aagcgaactt gcgggccaag taaaacaacg tcaacttcgt tatttttaat tttcccttta
      181 gcctctgcaa tcgccaatgc ggaaatatta acttcaagtg aaatagcgtt agcatgatca
      241 atcatacgtt taaccagcat actggttgac atgcccgcag cgcaaactaa caatattttt
      301 ttcatatttt cccttactgg tatataacag attacattgt ttaaatatca tatcccgcag
      361 tagctatacg tcctggtaca ttgatcacag taatacagtg ataaagttgt tcaacatcag
      421 ggtgattatt aaacgactcg gccatacgat aaaccaatca ggactcaact ccttgttttc
      481 aatggctctt aaaaggtgca aaagtgattt ttcaaaagat tgcccgctta cttaaatccg
      541 aaatcaatgg caattcatgg catgttggtg atttgttgcc gtcagaagcg gaactcgctg
      601 ttcgttataa tgtttcgcgt aataccctgc gtaaggcatt atccttgctg gaaggcgaag
      661 gaattattca cagaaagcat ggttcaggaa catacattca aaaaaagaat tttgttgcac
      721 acattgatca catgaacagt ttcagtgaaa ttgcgcataa aagcggcaaa gaggcaggga
      781 gtcagattat gaaatttgaa gtgcaggatg cttcacccac tattgcgact gagctgaatt
      841 tagtgactgg tgagcaggtt tattacataa agagactgcg attcattgag gataatgcag
      901 cacaattaga agaaacgtgg atgtcagtag cacgttttcc tgatttaacc gtgtcacata
      961 tgcaaaaatc caaattttca tatattgaga atgaatgcgg gatcaaaatc attggcacct
     1021 ttgaaacttt ctcccctact tttcctaccc cagaaatcgc cagtatttta cggatcagcc
     1081 cacgggatcc catacttaaa attcagaccc aggctgtgga cagtaactct attccgctgg
     1141 attattcgtt actttacagc aatattttcg agttccaggt aaagtacttt ttcccgcgat
     1201 aaaagcagac gcacagagtg ctgacatcac attctgtgcg tatggtttag cctctaacct
     1261 ttaccgcttt acgccaccgc aagccaaata cattgatata cagcccggtc ataatgagca
     1321 ccgcacctaa aaattgcaga cccgttaagc gttcatccaa caatagtgcc gcacttgcca
     1381 gtccgactac gggcaccagt aacgataacg gtgcaacccg ccaggtttca tagcgtccca
     1441 gtaacgtccc ccagatccca taaccaacaa ttgtcgccac aaacgccaga tacatcagcg
     1501 acaagatggt ggtcatatcg atagtaacca gactgtgaat catggatgcg gaaccatcga
     1561 gaatcagcga ggcaacaaag aagggaatga ttgggattaa agcgctccag attaccagcg
     1621 acatcaccgc cggacgcgtt gagtgcgaca tgatcttttt attgaagatg ttgccacacg
     1681 cccaactaaa tgctgcagcc agggtcaaca taaagccgag catcgccaca tgctgaccgt
     1741 tcagactatc ttcgattaac accagtacgc caaaaatcgc taaggcgatc cccgccaatt
     1801 gtttgccatg cagtcgctcc ccgaaagtaa acgcgccaag cacgatagta aaaaacgcct
     1861 gtgcctgtaa caccagcgaa gccagtccag caggcatacc aaagttaatg gcgcagaaca
     1921 gaaaggcaaa ctgcgcaaaa ctgatggtta atccataccc caagagcaaa ttcagtggta
     1981 ctttcggtcg tgcaacaaaa aagatggccg gaaaagcgac cagcataaag cgcaaaccgg
     2041 ccagcatcag cggtggcatg ttatgaagcc caactttgat gaccacaaaa tttagccccc
     2101 atacgaccac taccagtagc gccaacaccc catcttttcg cgacattcta ccgcctctga
     2161 atttcatctt ttgtaagcaa tcaacttagc tgaatttact tttctttaac agttgattcg
     2221 ttagtcgccg gttacgacgg cattaatgcg caaataagtc gctatacttc ggatttttgc
     2281 catgctattt ctttacatct ctaaaacaaa acataacgaa acgcactgcc ggacagacaa
     2341 atgaacttat ccctacgacg ttctaccagc gcccttcttg cctcgtcgtt gttattaacc
     2401 atcggacgcg gcgctacgct gccatttatg accatttact tgagtcacca gtacagcctg
     2461 agtgtcgatc taatcggtta tgcgatgaca attgcgctca ctattggcgt cgtttttagc
     2521 ctcggttttg gtatcctggc ggataagttc gacaagaaac gctatatgtt actggcaatt
     2581 accgccttcg ccagcggttt tattgccatt cctttagtga ataacgtaac gctggtggtg
     2641 ctcttttttg ccctcattaa ctgcgcctat tccgtttttg ctaccgtgct gaaagcctgg
     2701 tttgccgaca atctttcgtc caccagcaaa acgaaaatct tctcaatcaa ctacactatg
     2761 ctcaacattg gctggaccat cggtccgccg ctcggcacgc tgttggtgat gcagagcatc
     2821 aatctgccct tctggctggc agctatctgt tccgcgtttc ccatgctttt cattcaaatt
     2881 tgggtaaagc gcagcgagaa aatcatcgcc acggaaacag gcagtgtctg gtcgccgaaa
     2941 gttttattac aagataaagc actgttgtgg tttacctgct ctggctttct ggcttctttt
     3001 gttagcggtg cctttgcctc gtgcatttca caatatgtga tggtgattgc tgatggggat
     3061 tttgccgaaa aggtggtcgc ggttgttctt ccggttaatg ctgccatggt agttacgttg
     3121 caatattccg tgggccgccg ccttaacccg gctaacatcc gcgcgctgat gacagcaggc
     3181 accctctgtt tcgtcatcgg tctggtcggt tttatttttt ccggcaacag cctgctattg
     3241 tggggtatgt cagctgcggt atttactgtc ggtgaaatca tttatgcgcc gggcgagtat
     3301 atgttgattg accatattgc gccgccagga atgaaagcca gctatttttc cgcccagtct
     3361 ttaggctggc ttggtgccgc gattaaccca ttagtgagtg gcgtagtgct aaccagcctg
     3421 ccgccttcct cgctgtttgt catcttagcg ttggtgatca ttgctgcgtg ggtgctgatg
     3481 ttaaaaggga ttcgtgcaag accgtggggg cagcccgcgc tttgttgatt taagtcgaac
     3541 acaataaaaa tttaattcag ccttcgttta ggttacctct gctaatatct ttctcattga
     3601 gatgaaaatt aaggtaagcg aggaaacaca ccacaccata aacggaggca aataatgctg
     3661 ggtaatatga atgtttttat ggccgtactg ggaataattt tattttctgg ttttctggcc
     3721 gcgtatttca gccacaaatg ggatgactaa tgaacggaga taatccctca cctaaccggc
     3781 cccttgttac agttgtgtac aaggggcctg atttttatga cggcgaaaaa aaaccgccag
     3841 taaaccggcg gtgaatgctt gcatggatag atttgtgttt tgcttttacg ctaacaggca
     3901 ttttcctgca ctgataacgg atcgttgaca cagtagcatc agttttctca atgaatgtta
     3961 aacggagctt aaactcggtt aatcacattt tgttcgtcaa taaacatgca gcgatttctt
     4021 ccggtttgct taccctcata cattgcccgg tccgctcttc caatgaccac atccagaggc
     4081 tcttcaggaa atgcgcgact cacacctgcc gtcacggtaa tgttgatatg cccttcagaa
     4141 tgtgtgatgg catggttatc gactaactgg caaattctga cacctgcacg acatgcttct
     4201 tcatcattag ccgctttgac aataatgata aattcttcgc ccccgtagcg ataaacagtt
     4261 tcgtaatcac gcgtccaact ggctaagtaa gttgccaggg tgcgtaatac tacatcgccg
     4321 attaaatgcc cgtaggtatc attaaccaat ttaaatcggt caatatccaa caacattaaa
     4381 taaagattca gaggctcagt gttgcgtaac tgatgatcaa aggattcatc aagaacccga
     4441 cgccccggca atcccgtcaa aacatccata ttgctacgga tcgtcagcaa ataaattttg
     4501 taatcggtta atgccgcagt aaaagaaagc aacccctcct gaaaggcgtc gaaatgcgcg
     4561 tcctgccagt gattttcaac aatagccagc attaattccc gaccacagtt atgcatatgt
     4621 tgatgggcag aatccattag ccgaacgtaa ggtaattcat cgttatcgag tggccccaga
     4681 tgatcaatcc accgaccaaa ctggcacagt ccataagaat ggttatccgt tatttctggc
     4741 ttactggcat ctctcgcgac cacgctgtga aacatactca ccagccactg gtagtgggca
     4801 tcgatagcct tattgagatt taacaagatg gcatcaattt ccgttgtctt cttgatcatt
     4861 gccactcctt tttcacagtt ccttgtgcgc gctattctaa cgagagaaaa gcaaaattac
     4921 gtcaatattt tcatagaaat ccgaagttat gagtcatctc tgagataaca ttgtgattta
     4981 aaacaaaatc agcggataaa aaagtgttta attctgtaaa tcacctctgc attatcgtaa
     5041 ataaaaggat gacaaatagc ataacccaat accctaatgg cccagtagtt caggccatca
     5101 ggctaattta tttttatttc tgcaaatgag tgacccgaac gacggccggc gcgctttttt
     5161 tatccagact gccgctaatg ttgatcatct ggtccggctg aacttctcgt ccatcaaaga
     5221 cggccgcagg aataacgaca ttaatttcac cgctcttatc gcgaaaaacg taacggtcct
     5281 ctcctttgtg agaaatcaaa ttaccgcgta gtgaaaccga agcgccatcg tgcatgtttt
     5341 ttgcgaaatc aacggtcatt ttttttgcat catcggttcc gcgatagcca tcttctattg
     5401 catgaggcgg cgatggcgct gcatcctgtt ttaaaccgcc ctggtcatct gccaacgcat
     5461 aaggcatgac aagaaaactt gctaatacaa tggcctgaaa tttcatacta actccttaat
     5521 tgcgtttggt ttgacttatt aagtctggt
//
LOCUS       DAZKIV010000214         5536 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZKIV010000214 DAZKIV010000000
VERSION     DAZKIV010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.216"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..436
                     /locus_tag="ACHOT6_004408"
     CDS             <1..436
                     /locus_tag="ACHOT6_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9949506.1"
                     /translation="EQSHQLQQPEADSAIQREADRVVPENTGQPVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAILDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDTEIPPGFRG"
     gene            443..1189
                     /locus_tag="ACHOT6_004409"
     CDS             443..1189
                     /locus_tag="ACHOT6_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9949507.1"
                     /translation="MKNIAAAGVLERIRRLAPQASVPPYRTVEEWREWQLVEGRKRSE
                     EINRQNHQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            1211..1927
                     /locus_tag="ACHOT6_004410"
     CDS             1211..1927
                     /locus_tag="ACHOT6_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9949508.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGLQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            1960..2241
                     /locus_tag="ACHOT6_004411"
     CDS             1960..2241
                     /locus_tag="ACHOT6_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFP9949509.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            2238..2465
                     /locus_tag="ACHOT6_004412"
     CDS             2238..2465
                     /locus_tag="ACHOT6_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949510.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            2458..2769
                     /locus_tag="ACHOT6_004413"
     CDS             2458..2769
                     /locus_tag="ACHOT6_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949511.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            2897..3115
                     /locus_tag="ACHOT6_004414"
     CDS             2897..3115
                     /locus_tag="ACHOT6_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFP9949512.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            3117..3674
                     /locus_tag="ACHOT6_004415"
     CDS             3117..3674
                     /locus_tag="ACHOT6_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9949513.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            3965..4120
                     /locus_tag="ACHOT6_004416"
     CDS             3965..4120
                     /locus_tag="ACHOT6_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFP9949514.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            4240..4584
                     /locus_tag="ACHOT6_004417"
     CDS             4240..4584
                     /locus_tag="ACHOT6_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFP9949515.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            4706..4978
                     /locus_tag="ACHOT6_004418"
     CDS             4706..4978
                     /locus_tag="ACHOT6_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949516.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            4980..>5536
                     /locus_tag="ACHOT6_004419"
     CDS             4980..>5536
                     /locus_tag="ACHOT6_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9949517.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT         1458 a         1214 c         1611 g         1253 t
ORIGIN      
        1 ggagcaatca caccaactgc agcagcctga agccgattcc gccattcagc gggaagccga
       61 tcgggtagtc ccggaaaaca ccgggcagcc tgtgggacga gtggattatc cggatgtgtt
      121 cgaacaggtc tggcgggaat acccgttgcg tgctggggca aacccgaaga aatccgcttt
      181 cagtgcctgg aaggccagat tacgcgaggg ggtgccacca gaggccatac tggatggtgt
      241 gaggcgttac gcaagatacc tggcggctac cgggaaaacg ggaacggaat ttgttcagcg
      301 agcgacgacg ttttttgggc cggaccggaa ttttgaaaac ccctggttgc tcccggtaag
      361 cggcacgaac aaccagcgtt gtgtgaatca tatttctgaa ccggataccg aaattccgcc
      421 gggattcagg gggtgatgtg gcatgaaaaa cattgcggca gccggggttc ttgaacgtat
      481 tcgcagactt gcaccacagg cgtcggttcc accgtaccgg acggtggagg agtggcggga
      541 atggcaactt gttgaaggac gaaaacgcag cgaggagatt aaccgccaga atcaccagtt
      601 gcgggtggaa aaaatcctga atcgttcggg catccagcct ctgcacagca aatgctcgtt
      661 tgcgaattat caggtgcaga acgacgggca aaaatacgcg ctgagccagg ccaaatccat
      721 agctgacgaa ctgatgaccg ggtgcacgaa ttttgtgttc agcggtaaaa ccggcaccgg
      781 gaaaaatcac cttgcagcgg cgatgggtaa ccggctgatg gcgaaggggc gcagcgtgat
      841 tatcgtcacc gtgtctgatg tcatgagcgt gttgcatgac agctacgaca acggcaaatc
      901 cggggaaaaa tttttacagg agctttgcag tgttgatttg ctggtcctgg atgaaatagg
      961 cgttcagcgg gagacgaaaa acgagcaggt ggtattacac cagataattg atcgccggac
     1021 agcatcactg tgcagtgtcg ggatgttaac aaacctgaat catgccgcaa tgagcacgct
     1081 tcttggtgag aggattatgg accgcatgac catgaacggt ggtcggtggg tgacgtttaa
     1141 ctgggatagc tggcgtccaa atgtcagcaa tcagagggtt gtgaagtaat ttttgttgga
     1201 ggacgtttta atggaaactg tatttgacgc actgaaagca ctgaaaaaag cctcttcaca
     1261 ggtagtggca tcgcgccttg gaatcagccg cgaagatgct gtcaacgaac tgtggaaact
     1321 gaagcgccgt ggtgaagcgg ataacaaggg ttcgatgtgg tggctgattc aggctggtga
     1381 aagtgaacca gtgtcaccgg taccgaaagt gacagcgcaa atgctgactg aggcgattga
     1441 acatcatggc ctacaaacgg cggatgagct ggcactgatg ttcgggatta cctcccgccg
     1501 ggcgaattca tcactggcca tggcaatcag caaagggcgt ctgattcgcg tgaatcaggg
     1561 cggtaaattt cggtactgca taccgggcgc tgatttaccg gcagagccga aagccgcatc
     1621 catagcggaa acggatggta aagcctttcc tcagccagca ggtgttgcgt taccagtcgg
     1681 ggaagcggaa acacaggaag aaataaaaac ggaaagtgtg gcggtcacag tgcagtcaca
     1741 gccgtcgttc accagaaagc atccggatgg tctgatttta ccatcgctgc atgtggctaa
     1801 ccgcgagctg cgccgggcaa aaggtcaggt tcagaagtgg gagcgagtct gcgccgcgct
     1861 gcgggagctg aacaagtgcc gggatattct ccgggatatt accgccacca gagaacagca
     1921 gcggtgagtg ggtggaagac gtggtgccgg gcggaaatca tgatactccg gcagtgtgcg
     1981 ggaacgagga aggtaaaaag cgttggcgca cttatcggac gaactgaagc ggcagtgaga
     2041 acgaaggcac gggagctggg catcagcatg atgttacgtg gtgattttca cccgtcggca
     2101 aaatattctc agcgtgatat tgagctggcg cggcaactgc atcagagagg catgcaaaga
     2161 agggaaattg ccagaaaatt aggcatgccg ctgcgcatag tgaataacta cgtttatttc
     2221 gacaggaggg tgtctgcgtg aaaatcctgt atcaggatta cggcccggtg gggcaggtgg
     2281 ttatcagcag tactgtaatg gagtttcgga agcataaccg tgtggtggat gctgtgctgt
     2341 taacctgtcc ggggatatcg gcgagtcgtg caggtgtgtt tattatgaag acgaaattat
     2401 atggcagtaa ggcgtggata aagaaggcgt atcgtgtagc gttgcaggag gttaacagtg
     2461 agtaaaatta aagaaatgcc ggtagttcgt gacggatatg gctactggac acatcctgaa
     2521 tatgaaaaat tctgtgatgg tcgggaatat atttcaacgg aagagtttaa cgcctggatg
     2581 gaggaaaata atcttcaata cgtcctctgc ttcagagatg aaggatgtgc tgaccttgat
     2641 gcgtgtgatg ctgatatttc tgcatgggaa ccggaacgac cagagggcga tggctggttt
     2701 attggttcca ttcatgatac ggaagatggc ccggtttgtg tctggttgcg aaataaggct
     2761 gaagcataaa ggcgataaac caactaacaa ctaaatactg aagatttaaa tcagaaacga
     2821 tttttattaa atccttaacc ggagggattc ctgcaccctc agaacatcag gaggccgccc
     2881 gaaagggcgg taagaaatga aacattattt agaaaaaaat tacccacgaa agagcagaac
     2941 aacagagttt ctgtttttca ttctgtttat agtgttgatg ataccgatat ccccgctatt
     3001 actggtctgg ataattggaa ggacatttga accagttatt gagctatata ccgatgtgac
     3061 atgggaatca ttcagcgcac tgcacaataa aattaatccg tataaggaaa actgatatga
     3121 gcactattac cagagaacgc gcggagatta aatcatacat cacaggcttc ctgagcgact
     3181 cggcgcacga taacaagtct tcagacagcc tgctggctaa tgtgtttcgt atcgcgctgg
     3241 catcactgga agcagagccg atagcaatgg tagtgcctga tgaaatggat ttgcttacct
     3301 gccatctcga cggtgtaact aaaacatatg ctgatggctg gaacgcctgc cgcgtcgcca
     3361 tgcttcaggc cggaaacttt cgggaaaata agaattcgtc aaccaacaat tttcgggaaa
     3421 tctcggaaac gtcaaccaga tctccgataa ctctggatgg ctggataagc tgtactgagc
     3481 gaatgcctga aaagagccag aacgtgctta tttcgatgaa tatcgatagc gaggctgggc
     3541 cattaatata ttccgcacgc tatctcggag gcacgttccg gcgcggaggt atagcagtta
     3601 gtccgggtaa tgatcttagg caagcaaccc actggatgtc gctaccagaa ccgccgcagg
     3661 aggtgaatca atgacctggc ctgaagcatt cacaacggta ggaattgcga tggcggtggc
     3721 gctggtggtg tattcgattt gccgctgggg ataaaaacgg tttgcgggaa aaggatagtt
     3781 aagtagaatt gcagcgggtg cttgaggcta tctgcctcgg gcatgaacac caacggcaga
     3841 tagagaaaag ccccagttaa cattacgcgt cctgcaagac gcttaacatt aatctgaggc
     3901 tcaatccatg ctgaacacat gtaggttagc ctcttacgtg ccgaaaggca aggagaagca
     3961 ggctatgaag cagcaaaagg cgatgttaat cgccctgatc gtcatctgtt taaccgtcat
     4021 agtgacggca ctggtaacga ggaaagacct ctgcgaggtg cgaatccgaa ccggtcagac
     4081 ggaggtcgct gtcttcgtag actacgaatc tgagaagtaa gagaccaggc gggggagtaa
     4141 tctcccgcca cctctgatgt gtcaggcatc ctcaacgcac ccgcgcttta ccatactgaa
     4201 aatgctgttt gaatgttcat ctctgaaaga ggactatgaa tgaaaaaggt attgattgca
     4261 gcacttattt ccggtgtgtc ttttggcgct tttgcacagc agggtggttt ccaggggcca
     4321 gaagcagagc gttcaacagt agcgcaggca aaagaactga aggatgatgc atgggttatc
     4381 cttgaaggga gcatcgttaa aaaagtgggt gatgaacgtt atgagtttcg tgacaatagc
     4441 gggacaattg tcacggatat tgatgacagc gtatgggccg ggcagaatgt ttctccgaaa
     4501 gacaaagtaa gaattgaggg tgaaattgat aaagacctga gcagtgttga agttgatgta
     4561 aaggcactga aattattaaa gtaaccgccc ctgcttgtta agcccgtctt actgacgggt
     4621 tttctgtttg tacattccgg cgtattgcct tacaattcgc gcagtcagcc tgaacaactg
     4681 acacctgctg tcaccggaga atccgatgac acaacacata aaatcccaca attctgaagc
     4741 cgacccggaa attaagcagg ggaggcgttt tcgtgcgcct cagtatggct ggtttcacta
     4801 tctgttctgt acgatcgatg aggcagatat gcttcaagag gcgtatctgc gtcgcggtgt
     4861 ccgtgtggag cggagtctga acgctgatcg tctgacctgg accgtttctg tatatcttcc
     4921 tgttcgtgca catctgccac ggacacatgc ctgctaccgt cagcgcgtct ggaggtaatg
     4981 tgcgggtatt acttcgacct gttctggttc cggaactcgg gctggtggtc cttaagcccg
     5041 gtcgtgaatc attgccagtt tttcatcgcg gcagggtgct ggtggagccg gaaccgaaaa
     5101 acatgcgggc gctgccatct ggagcggttc ctgctgttcg ccagccgctg gcggaagata
     5161 aatcactgct gccatttttc agcgatgagc gggtgattcg tgcagctggc ggcgctggtg
     5221 cactgtctga ctggttatta cgtcacgtga aatcctgcca gtggccacac ggcgattatc
     5281 atcacagcga aaccgttatt cacagttacg gtgctggcgc aatggtgttg tgctggcact
     5341 gcgacaacca gctgcgcgac cagacctccg aatcacttga gcaacttact caacaaaatc
     5401 tgacagcctg gatgattgac gtcatacgcc atgtaatgaa tggcacgcag gagcgggaat
     5461 tatcgctggc tgaattatcc tggtgggcag tctgcaatca ggtggtggac gcattacctg
     5521 aggcagtatc gcgtcg
//
LOCUS       DAZKIV010000215         5526 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZKIV010000215 DAZKIV010000000
VERSION     DAZKIV010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.217"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            474..4373
                     /gene="espP"
                     /locus_tag="ACHOT6_004420"
     CDS             474..4373
                     /gene="espP"
                     /locus_tag="ACHOT6_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter EspP"
                     /protein_id="HFP9949518.1"
                     /translation="MNKIYSLKYSHITGGLIAVSELSGRVSSRTTGKKKHKRILALCF
                     LGLLPSSYSFASQMDISNFYIRDYMDFAQNKGIFQAGATNIEIVKKDGSTLKLPEVPF
                     PDFSPVANKGSTTSIGGAYSITATHNTKNHHSVATQNWGNSTYKQTDWNTSHPDFAVS
                     RLDKFVVETRGATEGADISLSKQQALERYGVNYKGEKKLIAFRAGSGVVSVKKNGRIT
                     PFNEVSYKPEMLNGSFVHIDDWSGWLILTNNQFDEFNNIASQGDSGSALFVYDNQKKK
                     WVVAGTVWGIYNYANGKNHAAYSKWNQTTIDNLKNKFSYKVDMSGAQVATIENGKLTG
                     TGADTTDIKNKDLIFTGGGDILLKSSFDNGAGGLVFNDKKTYRVNGDDFTFKGAGVDT
                     RNGSIVEWNIRYDNKDNLHKIGDGTLDVRKTQNTNLKTGEGLVILGAEKTFNNIYITS
                     GDGTVRLNAENALSGGEYNGIFFAKNGGTLDLNGYNQSFNKIAATDSGAVITNTSTKK
                     SVLSLNNTADYIYHGNINGNLDVLQHHETKKENRRLILDGGVDTTNDISLRNTQLSMQ
                     GHATEHAIYRDGAFSCSLPAPMRFLCGSDYVAGMQNTEADAVKQNGNAYKTNNAVSDL
                     SQPDWETGTFRFGTLHLENSDFSIGRNANVIGDIQASKSNITIGDTTAYIDLHAGKNI
                     TGDGFGFRQNIVRGNSQGETLFTGGITAEDSTIVIKDKAKALFSNYVYLLNTKATIEK
                     GADVTTQSGMFSTSDISVSGNLSMTGNPDKDNKFEPSIYLNDASYLLTDDSARLVAKN
                     KASVVGDIHSTKSASIMFGHDESDLSQLSDRTSKGLALGLLGGFDVSYRGSVNAPSAS
                     ATMNNTWWQLTGDSALKTLKSTNSMVYFTDSANNKKFHTLTVDELATSNSAYAMRTDL
                     KNSDKLVVNKKLSGKDNILLVDFLNKPSGEKLDIELVSAPGNSSKDVFKGSEQAIGFS
                     NVTPVITTRETDDKITWSLTGYNTVANKEATRNAAALFSVDYKAFLNEVNNLNKRMGD
                     LRDINGEAGAWARIMSGTGSASGGFSDNYTHVQVGVDKKHELDGLDLFTGFTVTHTDS
                     SASADVFSGKTKSVGAGLYASAMFDSGAYIDLIGKYVHHDNEYTATFAGLGTRDYSMH
                     SWYAGAEAGYRYHVTEDTWIEPQAELVYGSVSGKQFAWKDQGMHLSMKDKDYNPLIGR
                     TGVDVGKSFSGKDWKVTARAGLGYQFDLLVNGETVLRDASGEKRIKGEKDSRMLMSVG
                     LNAEIRDNVRFGLEFEKSAFGKYNVDNAVNANFRYSF"
     gene            complement(4544..4821)
                     /locus_tag="ACHOT6_004421"
                     /pseudo
     CDS             complement(4544..4821)
                     /locus_tag="ACHOT6_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4908..>5526
                     /gene="tnpA"
                     /locus_tag="ACHOT6_004422"
     CDS             4908..>5526
                     /gene="tnpA"
                     /locus_tag="ACHOT6_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001690050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9949519.1"
                     /translation="MNARKAVLADNPELILRVLQLRFDESLSYPRISAQTGVSKTAIF
                     SLVRRFHQIFADWPLSGEYSCMQLARALFPGRYPSAPTVTQPVKTEKIRRNRFSPEFK
                     WRLVQQTLLPGACVAQIARENGINDNLLFNWRHLWRNGGLQPPGEHETSLLPVTLTPE
                     PDNKIPAPVQIPEQINTLPDSLCCELVLPAGTLRLKGELTPALLQT"
BASE COUNT         1646 a         1244 c         1245 g         1391 t
ORIGIN      
        1 gtgggcagat aaaaaacctg ttccaccatg gctgtggcga aaagaaaata aaaatgacaa
       61 caaacagtgc ataaatattg aatacattta ctgagcatgc aaaagaaaaa tacaaactaa
      121 tcccggcctt tcacagaatt tcctctatca gaggctgagt tggcaatctg ctcacccagg
      181 aaatagtaac acacaaaaac ataaagagac tctaatgaac aagcgcaata catattcttc
      241 atctttcata gcatctcact tctttaacaa gaattattat tatctacgcc tcctcctttc
      301 atatatccct catgaatgat taagataaac acactgtgtt tgttatccgt atgataacaa
      361 acacataaag gagtaaacat ttatctgaac gatgattaca atgttcacaa ctgtcagaga
      421 cacacctctg atatattaga aaaattaata tttcccttag aaatggagct tatatgaata
      481 aaatatactc tcttaaatac agccatatta caggagggtt aatcgctgtt tctgaattat
      541 ccggcagagt atcatcaaga acaactggta agaaaaaaca caaacgcata cttgcattat
      601 gttttttagg cttattacca tcctcatatt cttttgcgtc acagatggat atttcaaatt
      661 tctacatccg tgactatatg gattttgcac agaacaaggg catatttcag gctggcgcaa
      721 caaatattga aatagtgaag aaagatggct ccaccctgaa actaccggaa gtaccatttc
      781 ctgacttctc accggttgca aacaaagggt caaccacatc tattggtggt gcatacagta
      841 tcacagccac acacaatacg aaaaaccacc actcagttgc gacgcaaaac tgggggaaca
      901 gcacgtacaa acaaactgac tggaatactt cacatcctga ttttgcagta tcccgacttg
      961 acaagtttgt tgttgagacc cgaggtgcga ctgaaggcgc agatatttcg ttatcaaaac
     1021 agcaggcact tgaacgttac ggggttaatt ataaaggaga aaagaaactt atcgcattca
     1081 gagccggctc tggtgtggta tccgttaaaa aaaatggacg cataactcca tttaatgagg
     1141 tttcttataa gccagaaatg ttaaatggct ctttcgttca cattgatgac tggagtggat
     1201 ggctgatatt aaccaacaac cagtttgatg agtttaataa cattgcctct cagggtgaca
     1261 gcggttcagc actgttcgtc tatgataacc aaaagaaaaa gtgggttgtc gctggaactg
     1321 tatgggggat ttataattac gccaatggca aaaaccacgc agcatacagt aaatggaacc
     1381 agacaaccat tgacaatctg aagaacaagt tttcttacaa agtggatatg tcaggagctc
     1441 aggttgcaac cattgaaaat ggaaaactga caggcactgg cgcagacacc accgatataa
     1501 aaaataagga cttaatattt actggcggtg gagatatcct cctgaaatcc tcttttgata
     1561 atggtgctgg cggtcttgtc tttaatgata aaaagaccta tcgagtaaac ggggatgatt
     1621 tcacctttaa aggtgccggt gttgatacaa gaaacggcag catcgttgag tggaatatcc
     1681 ggtatgataa taaagacaac cttcacaaaa ttggtgatgg cacattagat gtccgaaaaa
     1741 cccagaacac caacctgaaa acaggtgagg gtcttgtcat tcttggcgct gaaaaaacat
     1801 tcaataatat ctacataacc agtggtgatg gaactgtccg actgaatgca gaaaatgcac
     1861 tgtctggcgg tgaatacaac ggtattttct ttgcgaaaaa tggcggaact cttgacctga
     1921 acggatataa tcagtctttc aataaaattg ctgcaactga ttcaggtgct gtaataacca
     1981 atacgtcaac caaaaagtcc gttttatccc tgaataatac tgctgactat atctatcacg
     2041 gcaacataaa cgggaatctg gacgtacttc agcatcatga gacgaaaaaa gagaaccgtc
     2101 gtcttattct tgatgggggg gtggacacaa caaatgatat aagcctgcgc aatacacaac
     2161 tgtccatgca gggacatgcc actgaacatg ccatttatcg ggatggtgct ttctcttgtt
     2221 cactaccagc gcctatgcgc tttttgtgtg gcagtgatta tgttgcagga atgcaaaata
     2281 cagaagctga tgctgtaaaa caaaacggaa atgcctataa aaccaacaat gctgtctctg
     2341 atttatcgca gccagactgg gaaaccggaa cattcagatt tggaacgcta catcttgaaa
     2401 attccgattt ttctattggt cgtaatgcaa atgtaatcgg ggacattcag gccagtaaat
     2461 caaacattac tattggtgac actacagcat atattgattt gcatgctggt aaaaatatta
     2521 ccggtgatgg ttttggcttc cgccagaata ttgtgcgtgg aaactcacaa ggagaaacgc
     2581 tgtttacagg agggatcaca gcagaagaca gcactatcgt tattaaagat aaagcaaaag
     2641 cattattttc aaattatgta tacctgctga acacaaaagc aaccatagag aaaggtgctg
     2701 atgtgacaac tcaaagtggt atgttctcca cgagcgatat cagcgtctct ggtaatctgt
     2761 ccatgacagg caatcccgac aaagacaata aattcgagcc ctcaatatat ctgaatgatg
     2821 cttcttatct actgactgac gactccgcca gactcgttgc caaaaataaa gcatctgtgg
     2881 tgggagatat acactccact aaaagtgcat ccatcatgtt tggtcatgat gaaagcgacc
     2941 tctcgcagtt gtctgacaga acctcaaaag ggcttgcact tggtctttta ggtggctttg
     3001 atgtctcata tcgcggttca gtcaatgccc cgtcagcatc tgccactatg aacaacacct
     3061 ggtggcaact aaccggagat tctgcgctga aaacactgaa aagtacaaac agcatggtct
     3121 atttcactga cagcgcaaac aataagaaat tccatacgct gacggtcgat gaactggcaa
     3181 ccagcaacag cgcctatgcg atgcgtacgg acctgaaaaa ttcggataag ctggtagtaa
     3241 acaaaaagct gtcaggtaag gacaatattc tgttggttga ttttctgaac aaaccctccg
     3301 gagaaaaact ggatattgaa ctggtgagtg caccggggaa cagcagtaaa gatgtcttta
     3361 aaggaagtga acaggcgata ggtttcagta atgtaacgcc ggtcattaca acccgggaaa
     3421 ccgatgacaa aataacatgg tcactgacag gctataacac ggttgcaaac aaggaagcaa
     3481 cccggaatgc cgccgccctg ttctctgttg actataaagc gtttctgaac gaggtcaaca
     3541 acctgaacaa acgtatgggt gacctgcgtg acatcaacgg cgaagccggt gcatgggcac
     3601 gcatcatgag cggtactggc tctgccagtg gtggtttcag tgacaactac acgcacgttc
     3661 aggtcggggt cgacaaaaaa catgagctgg acggactgga tttgtttacc ggtttcactg
     3721 tcacacacac tgacagcagt gcctccgccg atgttttcag cggtaaaacg aagtctgtgg
     3781 gggctggcct gtatgcttcc gccatgtttg attccggtgc ctatatcgac ctgattggca
     3841 agtatgttca ccatgataat gagtacactg caacctttgc cggactcgga acccgtgatt
     3901 acagcatgca ttcatggtat gccggtgcag aagcgggcta tcgctatcat gtcactgagg
     3961 atacctggat tgagccacag gctgagctgg tttacggttc tgtatccggt aaacagtttg
     4021 catggaagga ccagggaatg catctgtcca tgaaggacaa ggactacaat ccgctgattg
     4081 gccgaactgg tgtggatgtg ggtaaatcct tctctggtaa ggactggaaa gtgacagccc
     4141 gtgccggtct gggctaccag ttcgacctgc tggttaacgg cgaaaccgta ttgcgggatg
     4201 catctggtga aaaacgcatc aaaggtgaaa aggacagccg tatgctgatg tccgttggcc
     4261 tgaatgcaga aatcagggat aacgtccgct ttggactgga gtttgagaaa tccgcctttg
     4321 gtaagtacaa cgttgataat gcagtcaacg ctaacttccg ttactcgttc tgatactccg
     4381 tattcaaaag ggcctgaggg ccctttacgc tcagtgcctg ccgccctgat atgtctgaga
     4441 gacatttccg ggatgtcagt cgactggttt acttaactgc gatgactaaa gctggcagta
     4501 atgccggctt tttgtaagcg tcaacggagc accgtattga cgcttattta ttggtgagta
     4561 ctacgttcca tggcaggagt tcatcaactc ggttggaagg ccattccggc agtacgctca
     4621 ggatatggcg cagatacgct tccggatcga taccgttcag actgcacgaa gcgctctttc
     4681 cgcagcatta ttatccgcct ccgccagacc gtcatcactg taataacaga gggcatccca
     4741 ctgattcagt acatagctga acgcttcgcc cagtctggat tttttcgaca gagtaccatt
     4801 cttctccacc atccattcat gcgtaagcgc atcatttaaa ccgtctttcg cctccctttc
     4861 ctgtttccga tactaatgtc cattttcgca gtaaaaggac atttaagatg aatgcacgaa
     4921 aggcggtact ggcagataat ccggaattga tcctgcgtgt gctacagctg agatttgacg
     4981 agtcactgtc gtacccgcgc atttctgcgc agaccggagt cagcaaaacc gccatttttt
     5041 ctctggtgag gcgatttcac cagatattcg ctgactggcc tctttccggt gaatattcct
     5101 gcatgcaact ggcccgggct cttttccctg ggcgataccc ttcagccccg acagtgactc
     5161 agcctgtgaa aacagagaaa atccgccgga accgattttc accggagttt aaatggaggc
     5221 ttgttcagca aactctttta cccggtgcct gtgtcgcaca aatagctcgc gagaacggaa
     5281 tcaacgataa cctgctcttt aactggcggc atctctggcg taacggcggc ctgcagccgc
     5341 ccggcgaaca tgaaacatcg ctacttcccg tgacgttaac gccggagccg gataataaaa
     5401 tcccggcacc agtgcagata cctgaacaga taaatacact gccagacagt ctgtgctgcg
     5461 agctggttct gccggccgga actctcaggc tgaaaggtga actgacaccg gcgttattac
     5521 agacac
//
LOCUS       DAZKIV010000216         5519 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZKIV010000216 DAZKIV010000000
VERSION     DAZKIV010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.218"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            109..312
                     /locus_tag="ACHOT6_004423"
     CDS             109..312
                     /locus_tag="ACHOT6_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9949520.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            372..953
                     /locus_tag="ACHOT6_004424"
     CDS             372..953
                     /locus_tag="ACHOT6_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bro-N domain-containing protein"
                     /protein_id="HFP9949521.1"
                     /translation="MNKNIAVTGKGDARHVKKICDFRDLVVLRFDSVNVRVVYLNGDP
                     WFVAKDVCVALEISNSRDALKALDADEKKTVALSYGIRGNPNHSLISESGFYKLIARS
                     RKAVTPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRCKEQYQLSASQKGRE
                     LQACKRKKRELEEEEKRLIREYQPEFYFGNRIQ"
     gene            complement(1074..1253)
                     /locus_tag="ACHOT6_004425"
     CDS             complement(1074..1253)
                     /locus_tag="ACHOT6_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9949522.1"
                     /translation="MERDHINNKSQKLQARAPHEVVEAMEQVKETGESTAQFIVTAMR
                     GEIKRRQRRKAKEAE"
     gene            1450..1641
                     /locus_tag="ACHOT6_004426"
     CDS             1450..1641
                     /locus_tag="ACHOT6_004426"
                     /inference="COORDINATES: protein motif:HMM:NF033153.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFP9949523.1"
                     /translation="MRNHTTHPQGRDLHNLNKFTWRFIALSTAQPRVITIEATSEQEA
                     CQQSPDGCVMVFAARIRQG"
     gene            1645..2010
                     /locus_tag="ACHOT6_004427"
     CDS             1645..2010
                     /locus_tag="ACHOT6_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949524.1"
                     /translation="MHNLSISDLNCIQFDEKFTGQLLVHVENGRIVRNYHLPDGAIAG
                     SVEALLELAERARLIKPLTNHHDDDLHFTGRMVSHYENGVEVSRERLRDDCCFGTLPE
                     FIELLTSCGYQVIQGGKHA"
     gene            2003..2236
                     /locus_tag="ACHOT6_004428"
     CDS             2003..2236
                     /locus_tag="ACHOT6_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949525.1"
                     /translation="MRDDRFNSLKREFDGAQEDAAGALLSVAGMMKAAYFLINTSGYK
                     SEGEMILSIASDYAEYVAETRYRRKSTEDVSHA"
     gene            2229..2429
                     /locus_tag="ACHOT6_004429"
     CDS             2229..2429
                     /locus_tag="ACHOT6_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949526.1"
                     /translation="MHNHEAHVPVVLNVPDDFTGRVLVYLDKGKVKSQCRLKSNEIVG
                     SPEFFSELCIRAEIKPELLTGK"
     gene            2434..2724
                     /locus_tag="ACHOT6_004430"
     CDS             2434..2724
                     /locus_tag="ACHOT6_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFP9949527.1"
                     /translation="MKKKNSGFTASGLSRPEIRHGDIYRDTRRGGRVVIRHVTPGNIT
                     YRREAYEYDCVMPRRQFERDFILVENKQQAVARRAATNIKKIRAMLVAGGKK"
     gene            2721..4526
                     /locus_tag="ACHOT6_004431"
     CDS             2721..4526
                     /locus_tag="ACHOT6_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase-like DNA-binding domain-containing
                     protein"
                     /protein_id="HFP9949528.1"
                     /translation="MKLAPNLKKQPHDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGDSQLDELADLKIIDDGRYCVRLYKAGHIKPSNINAIGQKLAVAGVRDANYY
                     PEGMHSQKRENWHEYLERERQNLSDGLVIQLPVKKKTEDSAAPLALNQMGASQRGEVL
                     LARYGGELAINADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIKSAVD
                     TMKLSLPVMGNAARNLIGFSNGVFDTRTGDFREHDKNDWLLIASELPFSPPAEGETLA
                     THAPNFWKWLRRSVAENDRKADRVLAALFMVLANRCDWQLFLEVTGAGGSGKSVMAEI
                     CTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGGDKVA
                     IDPKHKAPYSTRIQAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDPMLAE
                     KIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYLMSSVM
                     CDGLLVGNAEIIPFSPRRYLYHAYLAYMRAHGFGKPVTLTRFGKDMPGAMAEYGREYM
                     KRKTKHGLRSNVTLTEDSEDWMPSCASVTNDDGKN"
     gene            4814..5059
                     /locus_tag="ACHOT6_004432"
     CDS             4814..5059
                     /locus_tag="ACHOT6_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949529.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            5056..5478
                     /locus_tag="ACHOT6_004433"
     CDS             5056..5478
                     /locus_tag="ACHOT6_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFP9949530.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVMAFGRQAEALERHRKGELLSVAGNMQISQWTGQNGETRQGWQVIADSVISA
                     RTARLGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
BASE COUNT         1534 a         1168 c         1530 g         1287 t
ORIGIN      
        1 tgatgcaatg ggtcggtgat ttttttgatg atgcgagaaa aggggtaatt aatagatccg
       61 gtggtatgaa aggtttaaga gtagtaaatg gttgaggagg ttcagcaaat gaataccaat
      121 gaagatattt tatttactaa agacgtaatg aaaatcttgc gatatggagc aatgagtgca
      181 ttcatcaatt tctggaaaga tgagaataat ggttttcctc agccgttcag aattggacga
      241 cgacatacct ggcaccgtag agatgtagaa gcatggttag ataaacaacg agaacaagcc
      301 aatccccact aataatatct ttcatacccc gcgtgcaatg cggggttttt tgtatgtgag
      361 gtaaaaaaga aatgaataaa aatattgccg tgacgggcaa gggtgacgca cgtcatgtaa
      421 aaaaaatctg tgattttcgt gatctggtcg ttctgcgctt tgatagtgtg aacgttcgcg
      481 tggtgtatct gaacggcgat ccgtggtttg ttgcaaagga tgtttgtgtt gctctggaaa
      541 taagtaactc acgcgatgct ctaaaagcac tagatgcgga tgaaaagaaa accgtcgctt
      601 taagctacgg tattcgagga aatccaaatc atagcctcat ttctgaatcc ggtttttaca
      661 agctgatcgc cagaagtcgg aaagctgtta ctcctggcac gttcgcccat cgtttcagta
      721 actgggtatt caggaatgtg ataccaggaa tcagaaaaac gggggcttat ggtatcccgt
      781 ggggcgcatt acaggacttt tctcgctgta aagagcaata tcagttaagt gccagtcaga
      841 aggggaggga gctacaggca tgtaagcgca aaaagcgtga gctggaggaa gaagaaaaaa
      901 ggctgatacg tgaatatcag cctgagtttt actttggcaa ccgcattcag tgacaaaaca
      961 aaggcgaccc ggatcagagg tcgccaatgg gaaaacacta aacataagcc caaacatcat
     1021 agcgatttgt tggctggtgg gcaatgtgat cagtgtgctg gcttgctggt ggactattcc
     1081 gcttctttgg ccttgcggcg ctggcggcgt ttgatctcgc ctcgcatggc tgtaacgata
     1141 aattgcgcag tgctctcgcc tgtttctttt acttgctcca tagcctcaac aacttcatgc
     1201 ggggcgcgag cctgtaattt ctgtgatttg ttattgatat ggtctctttc catttctggt
     1261 tccctttgtg attactggaa tccagtatac acgaaaaaga ccaaaacaaa acgcttgaag
     1321 tggattccac ttagtgatta tactggaatc cagttgagtg tttatcactc aatgcaaacg
     1381 aggctgaaaa ggattgccgt cctttcgcag cctctgacca ccaacgatag caacagtatc
     1441 gaggtagcta tgagaaatca taccacacac ccgcaagggc gggacttgca caacctgaat
     1501 aaattcacct ggcgttttat cgccctgagc acggcacaac cgcgcgtgat taccattgag
     1561 gccaccagcg aacaggaagc atgccagcaa tctcctgatg gctgcgtgat ggtattcgct
     1621 gcccgtattc gtcaggggta agtcatgcat aacctgtcaa tttctgacct taactgcatt
     1681 cagtttgacg agaaatttac cgggcagcta ctggtccatg tggagaacgg gcgcatagtg
     1741 cgtaattacc acctgccgga tggtgcaatt gccggaagcg ttgaagcatt gctggaactg
     1801 gcagaacgtg cgcgactgat taagccgtta acgaaccatc acgatgatga tctgcatttt
     1861 accggacgca tggtgagtca ctacgaaaac ggcgtcgagg tatcccgcga acggctgcgt
     1921 gatgattgct gtttcggcac actgccggaa tttatcgagt tgctgaccag ttgcgggtat
     1981 caggtcattc aggggggtaa acatgcgtga tgatcgtttt aattccctga aacgggaatt
     2041 tgatggcgca caggaagatg cagcgggcgc attgttgagc gttgctggca tgatgaaagc
     2101 tgcatatttt cttatcaata ccagtggcta caagtcagag ggtgaaatga ttcttagtat
     2161 tgcgtcagac tatgcggaat atgtggcaga gacgcgttac agaagaaaat ccacggagga
     2221 tgtaagccat gcataatcat gaagcgcatg tacccgtagt gcttaatgtg ccagatgatt
     2281 tcaccggacg cgtactggtt tacctggata aagggaaagt gaaatcacaa tgccgactga
     2341 aaagtaatga gattgttggt tctcctgaat ttttttctga actttgtatt cgtgcggaaa
     2401 taaaaccgga actgctgaca ggaaaataaa accatgaaaa agaaaaattc tggctttact
     2461 gccagcggtc tctctcggcc tgaaatcaga cacggagata tttaccgcga caccagacgt
     2521 gggggacgag tggttattcg tcacgttacg ccaggcaata tcacctaccg ccgtgaggct
     2581 tacgaatatg actgcgtaat gccgcgccgt cagtttgagc gtgattttat tctggttgaa
     2641 aacaaacaac aggcggtggc gagacgtgca gccacgaata ttaaaaaaat ccgggcaatg
     2701 ttggttgcag ggggtaagaa atgaaactgg caccgaactt aaaaaaacag ccacacgaca
     2761 aaatgaccga agtcattatt tttgcgggta gtgatgcctg ggcgcacgcg aaacaatggc
     2821 aggaacagga cgggcgactt gctggcgaca acgtgccacc tgtatggctt ggagacagcc
     2881 agcttgacga actggcagac ctgaaaatta tcgacgatgg tcgctattgt gtccggctgt
     2941 acaaggcagg ccacatcaag ccgtcaaata ttaatgccat cgggcaaaag ctggcggtgg
     3001 caggtgtacg ggatgcgaat tattaccctg agggaatgca cagccagaag cgggagaact
     3061 ggcacgaata tctggagcgt gagcgccaga atctttctga tggtctggtt attcagcttc
     3121 ctgttaagaa aaaaacagaa gacagcgccg caccactggc gcttaaccag atgggagcaa
     3181 gccagcgcgg cgaagttctc ctggcacgtt atggcggtga actggcaatc aatgcggact
     3241 ctgacaccgt tcaccactac aacggcgttg tatgggagcc agtacaggat aaagagttac
     3301 agcgggctat ggcgcagatt tttattgatg cggagatcag ttattcgcag aacgccatca
     3361 aatcggcggt cgataccatg aagttaagtt tgcccgtaat ggggaatgcc gcccgtaacc
     3421 tgattggatt cagtaacggt gtttttgata cccggacagg cgattttcgg gagcatgaca
     3481 aaaacgactg gctgttaatt gccagtgaat taccgttcag cccgccagca gagggggaaa
     3541 cgctggcaac gcatgcgccg aatttctgga aatggttgcg ccgttcggtg gcagagaatg
     3601 accgtaaagc ggatcgcgta ctggcggcgt tattcatggt gctggcgaac cgatgcgact
     3661 ggcagttatt tcttgaggta acgggggcgg gtggaagcgg taaaagcgta atggcagaga
     3721 tttgcaccat gctggcgggt aaggccaaca cggtatcggc aagcatgaag gcgctggaag
     3781 atgccaggga acgcgcgtta gtggttggct tttcgctgat tatcatgccg gatatgaccc
     3841 gctacgctgg tgatggcgca ggaattaagg ccattacagg cggtgacaag gtggcaatcg
     3901 acccgaagca taaagccccc tactcaacac gcattcaggc ggtcgtgttg gcggtgaaca
     3961 ataacgccat gtcattcagt gaccgcagcg gggggatctc acgtcgtcgg gtgatattca
     4021 atttttcgga ggttgtaccg gaaaacgaac gcgaccccat gctggcagaa aaaatagaag
     4081 gagagctggc ggttgtgatt cgtcacctgc ttactcgttt ttctgaccag gacgaagcta
     4141 aacgcctgct gtatgagcag cagaaatcag aagaagctct ggtgataaaa cgcgagggcg
     4201 attcgctggt ggacttctgc ggctatctca tgtcgtcggt aatgtgtgat ggtctgttag
     4261 tgggtaatgc cgaaattatt ccgttcagtc cgcgcaggta tctttatcac gcctatctgg
     4321 catatatgag ggcacatgga ttcggtaaac ctgtaacact gacgcgcttc ggtaaagata
     4381 tgccgggggc aatggcggaa tatggcaggg agtatatgaa acggaaaacg aagcacggtt
     4441 tgcgttcaaa tgtgaccctg acagaggatt cagaagactg gatgccatca tgtgcatcgg
     4501 tcacaaatga cgatggaaaa aattaaactt atggaataac tgttcaccac tgttcaccct
     4561 gcaataaatg tcttttatat cagtatatta tagggtgagc agttatttat gaactgttca
     4621 ccaaactatt cactgttcac ctttttgatt ctttattgag ctttaagggt gaacagtggt
     4681 gaacagttgg tgaatagttt ttgtgaaact gttcacccct taacattatg aataaaaagg
     4741 gaaaattgca aaaggtgaac aggtgaaggg ttgaaatgca aaaattttat tttattgctg
     4801 tgaggtaaag cctgtgacaa cgaagcacgc caaaaaacca caatctcacg cccttgattt
     4861 gacagaacac tggctgaggg tggcgataaa aatcatcgac cgcaacgccg gggaaggata
     4921 cgcgaaagca catcccgaac tgataagcgc attcatgacc acgacggcgg caaactttgc
     4981 cacgctgaca gaacgggaga ttgccgaagc ggaacaggta acaaccatca acgttaaaac
     5041 cggagaggtg gaatcatgac agcacagata gcagcttacg ggcggctggt ggacgacccg
     5101 caggtaaaac agaccagcaa gggcacacca atgacgctgg cacgtatggc ggtatcgttg
     5161 ccatgtagtc aggcgcagga tgggcaggct acgttatggc tatcggtgat ggcatttggc
     5221 agacaggccg aagcactgga aaggcaccgc aagggtgaac tcctgagcgt ggcgggtaac
     5281 atgcagatca gccagtggac tgggcagaac ggagaaacgc ggcagggctg gcaggttatc
     5341 gcagacagtg taatcagtgc gcgaacggcg cgactgggcg gcaaaaaagg tcaacagggc
     5401 caggctactg acgcgctgaa cagggcaaaa caacaggcgg ggaatgatga tccgtacggg
     5461 gataacatac cgttttaagc gacgagtgac agaagccgga gcaatccggc ttttttgcg
//
LOCUS       DAZKIV010000217         5474 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZKIV010000217 DAZKIV010000000
VERSION     DAZKIV010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.219"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..716
                     /locus_tag="ACHOT6_004434"
     CDS             18..716
                     /locus_tag="ACHOT6_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFP9949531.1"
                     /translation="MRDKAKRDLSHISIEIPKSVTQVIKEKIRDMIIHGDLSLGQPIS
                     ENELSNILAVSKTPIREAFILLSHNENLINIIPRSGTFVFSVTDKDINDLIKMRVILE
                     LGAIREAMEKNANNVTIELSSILSKSAKINAERDIQAYLKLDHDFHYVFVKYADNKYI
                     SQAHQLISARLLAIRYRLDFTTEYITNSNRGHATILDMLKNNNVEGVCNFITHHVSSG
                     FTERARKILSLSTQ"
     gene            794..1516
                     /gene="ubiG"
                     /locus_tag="ACHOT6_004435"
     CDS             794..1516
                     /gene="ubiG"
                     /locus_tag="ACHOT6_004435"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFP9949532.1"
                     /translation="MNTENLLKNNNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(1657..5391)
                     /gene="yfaL"
                     /locus_tag="ACHOT6_004436"
     CDS             complement(1657..5391)
                     /gene="yfaL"
                     /locus_tag="ACHOT6_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFP9949533.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQN
                     QAELNVGSTQQTFVHALTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLAGAQSMALTGDIVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGMLEVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTLTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADILPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLSDVTVNGNLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVLNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAKFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPLPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQMAGFLSMTVKW"
BASE COUNT         1383 a         1444 c         1256 g         1391 t
ORIGIN      
        1 taataagggg caaaggaatg agagataaag cgaaacgtga cttaagtcac atatcaattg
       61 agataccaaa atccgtaaca caggtcatca aagaaaaaat cagggatatg ataattcatg
      121 gcgatctcag tcttgggcaa ccgataagtg aaaatgaact atcgaatatt ctggcggtaa
      181 gtaaaacacc tattcgtgaa gcttttattt tacttagtca caatgaaaat ctgatcaata
      241 tcattccacg cagcggaaca ttcgtttttt ccgtaacaga taaagatatt aatgacctca
      301 taaaaatgcg agtaattctg gagctgggag caattcgtga agcaatggaa aagaatgcaa
      361 acaatgtcac cattgaatta agtagcatac tatcaaaatc agcaaaaatt aatgccgaac
      421 gtgatattca ggcttatctt aaattggatc atgactttca ttatgtgttt gttaaatacg
      481 ctgacaataa atacatctct caggcacacc aattgatatc cgcacgttta cttgctattc
      541 gttatcgact ggatttcact acggaatata tcaccaattc aaatcgtggc catgctacta
      601 ttttagatat gctaaaaaat aataacgttg aaggtgtttg taattttatt actcatcacg
      661 taagtagtgg atttaccgag cgcgcacgaa aaattctctc gttatctaca cagtaaaatg
      721 tgaatgagtt tataacactg gcacttttca cctgctatac tgcccgacaa cctttataga
      781 ctcttatgta acaatgaaca ctgaaaatct ccttaaaaac aataacgtag atcacgaaga
      841 gatcgctaaa tttgaagccg ttgcctcccg ctggtgggat cttgaaggtg agttcaaacc
      901 gctgcaccgc attaacccgc tgcgtctggg ctatattgcc gaacgtgctg gcggtttatt
      961 tggcaaaaag gtgctcgatg tcggttgtgg cggcggcatt ctggccgaaa gtatggcgcg
     1021 cgaaggcgcg acggtgaccg gtctggatat gggctttgag ccattgcagg tggcaaaact
     1081 gcacgcgctg gaaagcggca ttcaggtgga ttatgtgcag gaaaccgtgg aagagcacgc
     1141 ggcaaaacat gccgggcagt atgatgtggt gacctgcatg gagatgctgg agcacgtccc
     1201 cgatccgcag tcagtggtca gagcctgtgc gcaactggtg aaaccaggcg gcgatgtctt
     1261 tttctcgacg cttaaccgca acggcaagtc atggctgatg gcggtggttg gcgcggaata
     1321 tattttgcgc atggtgccca aaggcacgca tgatgtgaag aagtttatta aaccggcaga
     1381 attgctgggc tgggtggatc agaccagttt aaaagagcgg catatcactg ggctgcatta
     1441 caacccgatc actaatactt ttaaactcgg ccccggcgtg gatgtgaact atatgctgca
     1501 cacgcagaat aagtgaggtt gttgtttgga ttcatccggc aaccgcgcca atgcctgatg
     1561 cgacgcttgc cgcgtcttat caggcctaca aatgctcccc gtaggccgga taaggcgttt
     1621 acgccgcatc cggcaaccgt gccgactaga cagtgattac catttcaccg tcatcgacaa
     1681 aaaccctgcc atctgggcaa aatcatcact ccctttctgc cacgccacgc tgccgcgcag
     1741 ggacactcgc tgactgatat tgcccgtgac tcccactttt atttcacccc gtcgcttcac
     1801 cgcatcgtca ctgatagtgc tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata
     1861 ataattcaga tctaatgttg gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc
     1921 cagccgcgtc tgaatatcat ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt
     1981 aaaatcatcc tgctgcacgc cctgataaat cacctgcgcc tgcggttcaa tcaccacacc
     2041 acgccccggt aaccactgat acccggcttc cagcgaggcg ataatccccg acgagtggta
     2101 atgatctgtg ccatcgtctt gttcggaaac atcattgtta aaccacgcgt attgcagcca
     2161 gctatccagc caggccccct gcttctgatt accgtgctga taccagcttg aggtcagccc
     2221 aacggcataa ccgtggttct ggttatcggc gcgagttccg gtcatattcg agcggctctc
     2281 gccctgatta tcactgtagc caccaaccgc cccaagcatc cactcgccat catcgcccca
     2341 gcgcccgcta aacaggtcac cgctaagctg caccgtagaa gtgtcttcat gctgagccag
     2401 ttgccctgcc gccgtgtaat gataacgtcc gccgataaca cgtaaattca gcgtctgacc
     2461 attgccacct gcatgatcgc gtcgctccat cataaacgcc tgatttgccg cccgcagatt
     2521 attgagataa ccgccaactt tggcattcag caccggctgg taagcgggga ttggatcggg
     2581 tgttgggtca ggatcgggtg ttgggtcagg gtctggatca ggtggcaatg gtggcgttac
     2641 ttcttgcgat cgcagatacc agtcgttgtt atcttccacc agcgtgtagt catatgctcc
     2701 catattgacg tagccgctgc ctgccagact gaacttcgca ttgttttgaa actgcgtggg
     2761 atcggctgcg aaatcaacca ctttaatgcc tgtcgatgtc ggctcaccaa tccctgtaat
     2821 ggagttaacc accacagttg tgttgccagc agtattaccg ttcaacacca attgatcgct
     2881 taccgagtca tcgccgttta attcgctatc gagcagtagc gtaccgccgc cggtataatc
     2941 accgtttacc gtcagcgtat cgccagcgac gccattttgc aggctaaccg caccggacgt
     3001 attggtcaga ttgccattaa ccgtcacgtc ggaaagatag gtcaggctgt cagtttcaag
     3061 gttggtatta agctcacctg tcaacgtcac gccatcggtg gcattcacca gcgtcccatc
     3121 gccgttaaac agtgaggcat taagggcgac agaagtatcc tgcccggtca ggcgcaaaac
     3181 cgtaccatcg ctgatgtcga tagtgccgct ggaagtagca tcaatagatt gaatatcctg
     3241 atccgcgcca gtaacgaacg tcgccccatc accgacccac agtgacgaag cataaggaag
     3301 gatatccgcg acatcgcctt tcagggtgcc ttcttcgaca cgtaccgccg actgcgttgt
     3361 accgctggcg gtcagctcca gcgtacccgc ccccgtttta gtcaatgtgc taccctcatc
     3421 ctgatgctgc ccgctgctgt cagccatcag tgcgccccac tgcgtgtcta cccccgcatc
     3481 aactgccact tcaccgtcgg cgcgcatttc aatatcacgc ccatgctcag tcgtcgtaga
     3541 gcgagtatcg acattggcag taatctccat cacgctttct tgttgcttat cggtaaagat
     3601 aacctggcgg ttatagtggg tatctccaag ctgtgagttg tcgctcacct ccagcatacc
     3661 ggaggcgatt tgtgttgtgc ccaaataact attattgttt gccagactta cctgcccgtc
     3721 gcccgcatcg accaccacgt agacgccatc tttcccgtca ccgccgatat gcatatcgtt
     3781 tccacctgca agatctacca catcctgact gccgataacc gttccgctgc tgccgctgac
     3841 ttcaaggcca tcgttgtatg atgtgccact ctgccaggtg gagaaatcag agagatcgag
     3901 cacaccgccg tttaacacga tcgactgcgg atcgtcctgg agagcggtaa gatctgccgc
     3961 gtcgccttcc agcgaaagca ccgcaccatc atcaacgact atatcgccgg ttagcgccat
     4021 cgactgcgcc cctgccagca cgtagctgcc gttttgcgca atggtgagct gaccagcacc
     4081 ttcgatggtg ccagcaaaac tgccctgatt aacagtaacg ttgccaccag catcgatatt
     4141 taaagtgcca ttctgaaagc ccgtcaatgc gtgcacaaaa gtttgttggg tcgagccaac
     4201 gtttagctca gcctgattct gatactgatc aatactccct atcgtcagac cgtagcagtc
     4261 ttgcggatcg tcctggcaat gcgtatcgcc gacattcatc agggagttgc tgcggcccag
     4321 ggtaacttca ccgttttcaa tctgcatctc gccagtaaag tcattgttat ctgcattaag
     4381 taccagatcg ccggaacctg ttttggtgat taacccggta ccagcaatag agtcaacagc
     4441 tccgtcattc tctgtattgc cgataaccag cgtttttccg tcggcaatat caaaggtaac
     4501 ttcacttaag ccgagataca taaagccacc cgccgcagtg gaaggaccat ctccatagcc
     4561 tgctgcgctg ttgttctcat ccactaacac gccgccgttc tggctgtagc tgtcatcaac
     4621 agaaatatca ataagatagg gagccgtcgc gctattggta tatatcgccc cgccataacc
     4681 ttcggcagtg ttatttgtaa aggcggtgtt atttattatc gtataacctg aaggatgctt
     4741 gctgtcgcta ttattatcgg taacatcgat tgccccgcca tcgccatcac tgtaacttgt
     4801 tgatgtatat gcctggttgt tatcaaaaat aacatcactt aaataaacgt cattattaat
     4861 ggtataaatt gcgccacctt tgccatcatt agcgatgtta ttgcgaaaca tggcgttagt
     4921 gacacgtaaa tcgacggcac cagtatcatt agtaccagaa gaatagattg cgccaccata
     4981 gccgcctgcg acgttaccag aaaaaataac atcagtaaga ttcagcgttg agttttcttt
     5041 agcaaatatg gccccgccgt tattatattc tcctgtaacg gtgttattgg caaacagagt
     5101 cattccagtt tcattttctg gtaatagtga aaattccgct ccttgttgca aaaatacggc
     5161 tcctccgctg gcgttattgg tcatatccga aaaaaccagc cattgcccat cggcgacact
     5221 ccagtcctgc gtaatgcctg aaaggctttg cctgctggcg tgacaactcg ctttgatatc
     5281 atatccctgg cataaatcaa tggccgccgc gacaccgtta gcagagaaaa gagatgcaat
     5341 cattgacggg agtagagata aatactcctt gcgtagaaag ataatccgca tattaatctg
     5401 accatcattt tatataagac ggcatattta aacgcattat acaatatggg ttatctttct
     5461 tttgtaaatt gtcc
//
LOCUS       DAZKIV010000218         5399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZKIV010000218 DAZKIV010000000
VERSION     DAZKIV010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.220"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(150..293)
                     /gene="ypaB"
                     /locus_tag="ACHOT6_004437"
     CDS             complement(150..293)
                     /gene="ypaB"
                     /locus_tag="ACHOT6_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFP9949534.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            421..2706
                     /gene="nrdA"
                     /locus_tag="ACHOT6_004438"
     CDS             421..2706
                     /gene="nrdA"
                     /locus_tag="ACHOT6_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFP9949535.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            2853..3983
                     /gene="nrdB"
                     /locus_tag="ACHOT6_004439"
     CDS             2853..3983
                     /gene="nrdB"
                     /locus_tag="ACHOT6_004439"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFP9949536.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            3983..4237
                     /gene="yfaE"
                     /locus_tag="ACHOT6_004440"
     CDS             3983..4237
                     /gene="yfaE"
                     /locus_tag="ACHOT6_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFP9949537.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4291..4941)
                     /gene="inaA"
                     /locus_tag="ACHOT6_004441"
     CDS             complement(4291..4941)
                     /gene="inaA"
                     /locus_tag="ACHOT6_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFP9949538.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(5021..5124)
                     /locus_tag="ACHOT6_004442"
                     /pseudo
     CDS             complement(5021..5124)
                     /locus_tag="ACHOT6_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5119..5361
                     /locus_tag="ACHOT6_004443"
                     /pseudo
     CDS             5119..5361
                     /locus_tag="ACHOT6_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1361 a         1424 c         1311 g         1303 t
ORIGIN      
        1 gacatcaaca atcgcttaca ttcgcttata tattgaccac aactgataca tcagattatg
       61 tgatgactcg tgcttagatc aatttttgca atcattagca aaaagattaa taaaccatct
      121 atatcaattt atccaaccta ttctgccgtt caagaaatcg ccgaacagtt atttttaaca
      181 aatttttctc ttcccattga ctttcccgga caccttgtct gacctaaggt gcgcgaaagc
      241 cactttttcc ttcctgagtt atccacaaag ttatgcactt gcaagagggc cattttcaca
      301 ctatcttgca gtgaatccca aacatacccc ctatatatag tgttctaagc agcttcccgt
      361 actacaggta gtctgcatga aactattgcg gaaagaattc caaaaacagg tacgacatac
      421 atgaatcaga atctgctggt gacaaagcgc gacggtagca cagagcgcat caatctcgac
      481 aaaatccatc gcgttctgga ttgggcggca gaaggactgc ataacgtttc gatttcccag
      541 gtcgagctgc gctcccacat tcagttttat gacggtatca agacctctga catccacgaa
      601 accattatca aggcggccgc agacctgatc tcccgtgatg cgccggatta tcagtatctc
      661 gccgcgcgcc tggcgatctt ccacctgcgt aaaaaagcct acggccagtt tgagccgcct
      721 gcgctgtacg accacgtggt gaaaatggtc gagatgggca aatacgataa tcatctgctg
      781 gaagactaca cggaagaaga gttcaagcag atggacacgt ttatcgatca cgaccgtgat
      841 atgaccttct cttatgctgc cgttaagcag ctggaaggca aatatctggt acagaaccgc
      901 gtgaccggcg aaatctacga aagcgcccag ttcctttata ttctggttgc cgcgtgcctg
      961 ttctcgaact acccgcgtga aacgcgcctg caatatgtga agcgttttta cgacgcggtt
     1021 tccacattta aaatttcgct gccgacgcca atcatgtccg gcgtgcgtac cccgactcgt
     1081 cagttcagct cctgcgtact gatcgagtgc ggtgacagtc tggattccat caacgctacc
     1141 tccagcgcga ttgtgaaata cgtttcccag cgtgccggga tcggcatcaa cgccgggcgt
     1201 attcgtgcgc tgggtagccc gattcgcggt ggtgaagcgt tccataccgg ctgtattccg
     1261 ttctacaaac atttccagac agcggtgaaa tcctgctctc agggcggcgt gcgcggcggt
     1321 gcggcaacgc tgttctaccc gatgtggcat ctggaagtgg aaagcctgct ggtgttgaaa
     1381 aacaaccgtg gtgtggaagg caaccgcgtg cgccatatgg actacggggt acagatcaac
     1441 aaactgatgt atacccgcct gctgaaaggt gaagatatca ccctgttcag cccgtccgac
     1501 gtaccggggc tgtacgacgc attcttcgcc gatcaggaag agtttgaacg cctgtatacc
     1561 aaatatgaga aagacgacag catccgcaag cagcgtgtga aagccgttga gctgttctcg
     1621 ctgatgatgc aggaacgtgc gtctaccggt cgtatctata ttcagaacgt tgaccactgc
     1681 aatacccata gcccgtttga tccggccatc gcgccagtgc gtcagtctaa cctgtgcctg
     1741 gagatagccc tgccgaccaa accgctgaac gacgtcaacg acgagaacgg tgaaatcgcg
     1801 ctgtgtacgc tgtctgcttt caacctgggc gcaattaata gcctggatga actggaagag
     1861 ctggcaattc tggcggttcg tgcacttgac gcgctgctgg attatcagga ttacccgatc
     1921 ccggccgcca aacgtggagc gatgggtcgt cgtacgctgg gtattggtgt gatcaacttc
     1981 gcttactacc tggcgaagca cggtgtgcgt tactccgacg gcagcgccaa caacctgacg
     2041 cataaaacct tcgaagcgat tcagtattac ctgctgaaag cgtctaacga gctggcaaaa
     2101 gagcaaggcg cgtgcccgtg gtttaacgaa accacttacg cgaaagggat cctgccgatc
     2161 gatacctata agaaagatct ggataccatc gctaatgagc cgctgcatta cgactgggaa
     2221 gcattgcgtg aatcaatcaa aacgcacggc ctgcgtaact ccacgctttc tgccctgatg
     2281 ccgtcagaga cttcttcaca gatctctaac gccactaacg gtattgaacc gccgcgcggt
     2341 tacgtcagca tcaaagcgtc gaaagacggt attttgcgcc aggtggtgcc ggactacgag
     2401 cacctgcacg acgcctatga gctgctgtgg gaaatgccgg gtaacgatgg ctacctgcaa
     2461 ctggtaggta tcatgcagaa atttatcgat cagtcgatct ctgctaacac caactacgat
     2521 ccgtcacgtt tcccgtcagg aaaagtgccg atgcagcagt tgctgaaaga cctgctcacc
     2581 gcctacaaat tcggggtcaa aacgctgtat tatcagaaca cccgtgacgg cgctgaagac
     2641 gcgcaggacg atctggtacc gtcaatccag gacgatggct gcgaaagcgg cgcatgtaag
     2701 atctgatatt gagatgccgg atgcagcgtg aacgccttat ccggcctacg gctcggattt
     2761 gtaggcctga taagacgcgt cagcgtcgca tcaggcacag gatgcggcgt aaaatgcctt
     2821 atccggcatt aaactcccaa caggacacac tcatggcata taccaccttt tcacagacga
     2881 aaaatgatca gctcaaagaa ccgatgttct ttggtcagcc ggtcaacgtg gctcgctacg
     2941 atcagcaaaa atatgacatc ttcgaaaagc tgatcgaaaa gcagctctct ttcttctggc
     3001 gtccggaaga agttgacgtt tctcgcgacc gtatcgatta ccaggcactg cctgaacacg
     3061 aaaaacacat ctttatcagc aacctgaaat atcagacgct gctggattcc attcagggcc
     3121 gtagcccgaa cgtggcactg ctgccgctga tctcgatccc ggaactggag acctgggtcg
     3181 aaacctgggc gttctcagaa acgattcatt cccgttccta tactcatatc attcgtaata
     3241 tcgttaacga tccgtctgtt gtgtttgacg atatcgtcac caacgagcag atccagaaac
     3301 gtgcggaagg gatctccagc tattacgatg agctgatcga aatgaccagc tactggcatc
     3361 tgctgggcga aggtacccac accgttaacg gtaaaactgt gaccgttagc ctgcgcgagc
     3421 tgaagaaaaa actgtatctc tgcctgatga gcgttaacgc gctggaagcg attcgtttct
     3481 acgtcagctt tgcttgttcc ttcgcatttg cagaacgcga attgatggaa ggcaacgcca
     3541 aaattattcg cctgattgcc cgcgacgaag ccctgcacct gaccggcacc cagcatatgc
     3601 tgaatctgct gcgcagcggc gcggacgatc ctgagatggc ggaaattgcc gaagagtgta
     3661 agcaggagtg ctatgacctg tttgttcagg cagctcaaca ggagaaagac tgggcggatt
     3721 atctgttccg cgacggttcg atgattggtc tgaataaaga cattctctgc cagtacgttg
     3781 aatacatcac caatatccgt atgcaggcag tcggtttgga tctgccgttc cagacgcgct
     3841 ccaacccgat cccatggatc aacacctggc tggtgtctga taacgtgcag gttgctccgc
     3901 aggaagtgga agtcagttct tatctggtcg ggcagattga cgcggaagtg gacaccgacg
     3961 atttgagtaa cttccaactc tgatggcccg cgttaccctg cgcatcactg gcacacaact
     4021 gctgtgccag gatgaacacc cttcccttct ggcggcgctg gaatcccaca atgtggcggt
     4081 tgagtatcag tgtcgcgaag gttactgcgg ctcctgtcgc acccgtctgg ttgcaggtca
     4141 ggttgactgg attgccgaac cgttagcctt tattcagccg ggggaaattt tgccctgttg
     4201 ttgccgggca aaaggcgata ttgaaatcga gatgtgaatt ggttgtagtg ccagatacaa
     4261 cgcttatgcg tcttatctgg cctacaacga ttacatggcg tagtaatacg ctttcacctg
     4321 ctcccagtcc gctttgggga ttggctccag atatttttcc agctggcgga agtcatgatt
     4381 aatcgcttta tcgcgacgca agcggcgacg acttttctcc aggtcaagaa aaccagcttc
     4441 tgcattacct tctgttttca catagatatg gcgaacataa caacagccat gctgacgatt
     4501 aatgctatgc attttcttaa acgccagcgc cactgctttc aacatggctt gccgtacttc
     4561 gtcagaataa ggcgatactg catgctgggc ataccagtca gcaatgctga tgaaccccgc
     4621 catatcttca gtcaccagca acgctcgcca ttcaccctca attttcaccg cttcaccaaa
     4681 aacgatcttc ggcacgatga caccggcccg ttccagttct ttaattaccg caacctcacg
     4741 gacaatcgtt ggtcggccga acggataacg tacggaatga aacagatgat gcgtcatgcg
     4801 ctttacatac agctttttgc cgttgcgctc gacgcattgc accccgctca taccattacg
     4861 gcgatagtta ggctcttcaa cccagtcgcc ctctgttgcc caccagtgat taaattcgtc
     4921 gtactttgct gaaactgcca taccctatcg cctgtcattt ttattaacga caatgactat
     4981 aggtggttac ctgaggaaaa tcttaatgaa acgtgtcgta ttaatgaatt tcttttgcca
     5041 ctactagctt gacaccgctt tacccttcat ttgcagcgca tagattccac cagccactac
     5101 cactaatagt gcgcccagca gataaaatgt gaatttaatc aggcggatac agcgatgaat
     5161 tttattcgac agggattagg catcgcctta caaccagagt taacgctgaa aagcattgca
     5221 ggtgaattgt gttccgttcc tctcgaacca actttctatc gacagatttc gttgctggct
     5281 aaagaaaagc cggtagaagg cagtccactg tttttactac aaatgtgcat ggaacaatta
     5341 gtggcgattg gaaaaatttg atatgagaca ggatggcgca tgaacgccat cctgcagta
//
LOCUS       DAZKIV010000219         5386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZKIV010000219 DAZKIV010000000
VERSION     DAZKIV010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.221"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(40..318)
                     /locus_tag="ACHOT6_004444"
     CDS             complement(40..318)
                     /locus_tag="ACHOT6_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFP9949539.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(352..900)
                     /gene="epmC"
                     /locus_tag="ACHOT6_004445"
     CDS             complement(352..900)
                     /gene="epmC"
                     /locus_tag="ACHOT6_004445"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFP9949540.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(900..1709)
                     /locus_tag="ACHOT6_004446"
     CDS             complement(900..1709)
                     /locus_tag="ACHOT6_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFP9949541.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(1709..2533)
                     /gene="mepA"
                     /locus_tag="ACHOT6_004447"
     CDS             complement(1709..2533)
                     /gene="mepA"
                     /locus_tag="ACHOT6_004447"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFP9949542.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPKLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(2537..3622)
                     /gene="aroC"
                     /locus_tag="ACHOT6_004448"
     CDS             complement(2537..3622)
                     /gene="aroC"
                     /locus_tag="ACHOT6_004448"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFP9949543.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(3657..4589)
                     /gene="prmB"
                     /locus_tag="ACHOT6_004449"
     CDS             complement(3657..4589)
                     /gene="prmB"
                     /locus_tag="ACHOT6_004449"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFP9949544.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            4755..5306
                     /gene="smrB"
                     /locus_tag="ACHOT6_004450"
     CDS             4755..5306
                     /gene="smrB"
                     /locus_tag="ACHOT6_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFP9949545.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
BASE COUNT         1287 a         1447 c         1424 g         1228 t
ORIGIN      
        1 taagcgtcgc atcaggcatg gtgctccaga cgccgcaact tactgctgtg acgcctgctg
       61 aaacaggttt tcccacatat cggtcaccag cgcctggtca cgcggcgaca attcaccggc
      121 accaatggct ttttccagac tctggctaac ggtcgtatgc accgcctgag cggagtggtc
      181 atcaccactt tccagttctg cgatggctaa tgtcaggtgg ccacgcaaat acccactggc
      241 aaacaactca tcatcactgg catggtcaac cataccgtcg attaatgcca gaatgcgtga
      301 ttcaaactcc gcgatcatct tctttcctca ttgtaaaacg tggcaccggc ttcagttgag
      361 cgcttccggc cacgggaact gttccgccgt aagttccggc gtgtgataat aattttgtag
      421 tgctttaatg aagcgtgccg gacgctcggg aatgccattt gtcagataat ccatcacctg
      481 tgcatgtacc cgccgttgaa acaccacacg atccggttcg aagtcgcctt ccagattgtc
      541 gcaactgacg ttaaacggat atcccgccgc cacacagaac aaccagtcga gtgcctgcgg
      601 cttcacttca acatcttcaa actggctttg cgtttgggca tcacgcccat ccgggcaata
      661 ccagtagccg aaatcaacca gctcacggcg cgcttttccg gcaatacacc agtgcgaaat
      721 ctcatgaatt gcgctggcat aaaagccgtg ggcaaagaca attcggttat acggtacttc
      781 cgcatcagca ggaagataga tcggttcgtc gtcgccttta atcagacggg tattaaattc
      841 atcggcaaag cagctattaa aaatttcaat caactgctca tagtggtgtg tactgttcat
      901 tagttcatcc ccaaccagtg gaggatctcc tgtccgtggc tatcgtaaag taatttggca
      961 ctcatcaccg ccgagacgat aacgatcatc gggcggatca gtttttgtcc tttgctcagc
     1021 accagtcttg aacccatgcg cgcgccgagg aactgcccca ccagcatgac aaagcccgtc
     1081 gcccaaatca ctttgccgcc gagaataaac agcagcagac cgccaatgtt tgacgttgcg
     1141 ttgagtaatt tggcgtgagc cgtggctttg gcgaggttga atccgcacag cgtaacgaag
     1201 gccagcgcgt aaaacgatcc ggcagccggg ccaaagaatc cgtcataaaa accgacgcag
     1261 ccaccggcaa ttaacgcgaa gggtaaaccg tacatccggc gctggcgatc ttcttcaccc
     1321 agctttggca tcagcaagaa atagaggccg atacaaatta ccagaatggg caaaatctgc
     1381 cgtaagacat cagcctgaac gtattgcacc agcagtgcgc cgctcattga gccgacaaag
     1441 gtcatggcga tattgagttt ctgatcgctt aaactaacca ctttgcggcg aataaagtag
     1501 atagtagcgg aaatagagcc gccgcaggct tgcagtttat tggttgccag cgcattagcg
     1561 ggagacatcc ccgctgccat caatgccgga atggtgagta acccaccgcc accggcaatc
     1621 gagtcgataa atcccgccag catggcgaca aaaaagagaa ctcccagcaa cagcggggaa
     1681 accataaaca ggctattaaa cgtttccatt agatcacgtg ctcatccagt agcgcctggc
     1741 aggaaggcgg caacggaggc ggtgtcttct tctcaggctt tgttgttccc ggttttggag
     1801 gtgcaaacca gctttgcagt tctgccccgc aaccatcgcc tggtggcggt aaaggttgat
     1861 cttcacactc cagactatcg gcaggacaac gtaatcgtac atgcatatgc gcgcgatgct
     1921 ggaaccaggg tcgcactttg cgcaaccagt cgcgatcggt gcccgcatca aggcaaagtt
     1981 gttgtttaat cgccggatta acaaaaatgc gtgtgacgtc tttgtcctgg gcggcgagtt
     2041 tgatcaagct gaaaatttct ggcttccaca gcttcggaac aacgtgttta ccgtcgcggg
     2101 aaactaagtc cagtgcttgc gggcgcaaga gctgcgcgga ggtccagcga gttttcggca
     2161 gttgcagaaa gatatcgaca tccagtccgg tctggtggct ggcatgaccg ccgttgaaac
     2221 gcccaccagc gggcatcccc atatcgccaa tcagcaccgt acccatgccc agattgctca
     2281 cctggctact cagacgctgg ataaacatca ccagatccgg gtgaccgaaa tagcgacgct
     2341 gatcggtacg catgacctga taatgttcgg actgtatcgg cagcgtgtca gcgccgacga
     2401 tacagccatt agaaaaactg cctatcgatt gtgcgctacc cggcacaggt tgggttattt
     2461 tttgccacgg cgtcgctgcc aggctagcgc tactggcaag cagagccagc agcgcaatcg
     2521 cggttttgtt cattttttac cagcgtggaa tatcagtctt cacatcggca ttttgcgccc
     2581 gctgccgtaa caggtgatcc attaaaacga tcgccagcat cgcttcagcg atcggcactg
     2641 cacggatccc gacacaggga tcgtgacggc ctttggtgat catctcaact tcttcgccaa
     2701 agcggttaat agtgcgcccc ggcacggtaa tgctggaggt cggtttcagc gccatatgag
     2761 caatgatttg ctgcccgctg ctgataccgc cgagaatgcc gcccgcatgg ttgctctgga
     2821 aaccgtcttt ggtgatttcg tcgcggttct ggctgccgcg cagcgccact acgtcaaagc
     2881 cgtcgccaat ttccacgcct ttcaccgcgt tgatgctcat cagcgcatgg gcgatgtcgg
     2941 catccaggcg gtcaaatacc ggctcgccaa gtccggcggg gacgccactg gcaacaacgg
     3001 tgactttcgc gccgatggag tcgccctctt ttttcagcgc gcgcatcagt tcatctaacg
     3061 cgtcgatttt gtccgggtcc gggcagaaga acggattttg ttcaaccagc gaccagtctt
     3121 tgatttccag cggaatgtcg cccatctggg tcaagcagcc gcggatttca ataccaaatt
     3181 tctcggcgag atattttttg gcaatcgccc ctgccgccac gcgcatggcg gtttcgcggg
     3241 cggaagaacg tccaccgccg cgataatcgc gcagaccgta tttttgttcg taggtgtaat
     3301 cagcatggcc tgggcggaaa acgtctttaa ttgcgctgta atcctgagag cgctggtcgg
     3361 tattttcgat caacaagcca atgctggtgc cagtagtaac gccttcaaaa acaccggaga
     3421 gaattttgac ctgatccggc tcgcggcgct gggtggtata gcgcgatgtc ccagggcgac
     3481 gacggtcgag gtcatgttgc aggtccgctt ccgtcagcgg aatgcctggc ggaacaccat
     3541 cgacgatgca gccgagcgcc agcccatgcg attcgccgaa ggtggttacg cgaaagagtt
     3601 gtccaattgt gtttccagcc atcacggctc cgttattgtt gtgtttgcgt gtttacttaa
     3661 tctttataaa tcgcgaaata ttcacgggcc gccagcagct gctctttggt gagcataaac
     3721 acaccatcgc cgccgttatc aaactccagc caggtgaacg gaacatccgg atattgttcc
     3781 ataagatgta ccatgctgtt gccgacttca caaatcaaca cgccatcatc agcaaggtaa
     3841 tctgccgcgt ttccaagaat gcgacgcgtc agtttaaggc cgtcggtgcc agatgccagg
     3901 cccagttccg gctcgtgacg gtattcgttt ggcaggtcgg acatatcttc cgcgtcgaca
     3961 tacggcgggt tagtgacaat cagatcgtat tgcactttcg gcaaatcacg gaacagatcg
     4021 gaacgaatcg gaatgacgtt gtggatcaga ccgtgttctt cgatgttctg ttcggcaacc
     4081 gccagcgcgt cgggagagat atctaccgcg tcgacttccg cttccgggaa ggcataagca
     4141 caggcaatgg cgatgcagcc gctaccggta cacatatcta aaatatgctg cggttgcttg
     4201 ctgatgagtc cggcaaattt attgttgatc agttcaccaa tcggcgagcg cggcaccagc
     4261 acgcgttcat cgacgtaaaa ttcatgaccg cagaaccagg ctttgttggt caggtaagcc
     4321 accggaatgc gctcgttgac gcggcggatc acgcgttcaa caatacggtg tttttcgctg
     4381 gaggtcagac gcgcggtacg catatcttcc gggatatcca gcggcaggta gagcgatggt
     4441 aacaccagct gtacggcttc atcccacggg ttatcggtgc cgtgaccgta ccagatattt
     4501 gccgcgctga agcggcttac cgaccagcgc aacatgtcct gaatggtttg cagctcattt
     4561 actgcttcat caacgaaaat tttatccacg tattcctcca gggcatgatc gcaataattt
     4621 cggcggctag tttgccatga agatgacgat aaatcagcat tcacgcgcgg tgagtgagga
     4681 aaaatacgtt taaaacgatc gattgtgcta cgagtcgggt aaactgtagg aaaattagaa
     4741 atagagacag ataaatgaaa aagaaaacaa cacttagcga ggaggaccag gctctgtttc
     4801 gccagttgat ggcggggact cgcaagatta agcaggacac gattgtccac cgaccgcagc
     4861 gtaaaaaaat cagcgaagtg ccggtgaaac gcttgatcca ggagcaggct gatgccagcc
     4921 attatttctc cgatgagttt cagccgttat taaataccga aggtccggtg aaatatgttc
     4981 gcccggatgt cagccatttt gaggcgaaga aactgcgccg cggcgattat tcaccggaat
     5041 tgtttttgga tttacacggt ctgacgcagc tgcaggccaa gcaggaactg ggcgcgttga
     5101 ttgccgcctg tcgccgcgaa catgtgtttt gtgcctgcgt gatgcatggg cacgggaagc
     5161 atattttgaa gcaacaaaca ccgctgtggc tggcacaaca tccgcatgtg atggcatttc
     5221 accaggcacc gaaagagtac ggcggtgatg cggctttgtt ggtgttgatt gaagtggaag
     5281 agtggttgcc gccggagttg ccctgaatgc tggcagcaaa tgccggagca gcgtaaacgc
     5341 cctatccggc ctacaaatgt gtgataattc aataaattgt aattca
//
LOCUS       DAZKIV010000220         5383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZKIV010000220 DAZKIV010000000
VERSION     DAZKIV010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.222"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..671)
                     /locus_tag="ACHOT6_004451"
     CDS             complement(<1..671)
                     /locus_tag="ACHOT6_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9949546.1"
                     /translation="METVFDALKAMGKATSVELAARLDISREEVLNELWELKKAGFVD
                     KSVYTWRVADNNVQQEQPAPEETTTATEAKISECDLTATIEQRGPQTADELATLFGTT
                     SRKVASTLAMAISKGRLIRVNQNGKIRYCIPGDNLPAEPKVESVAETDGKAFPQPAGV
                     ALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNHVQKW
                     ERVCAA"
     gene            complement(705..1166)
                     /locus_tag="ACHOT6_004452"
     CDS             complement(705..1166)
                     /locus_tag="ACHOT6_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9949547.1"
                     /translation="MTEQQIRQVCRQCMDCCRAGETWPPDLAEFVALISKSGANPFGL
                     TVDAVMEEYCRWRNESWRYDGSDKYPWSQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPSGPTPIELLKQEYERRKAAGFV"
     gene            complement(1159..2199)
                     /locus_tag="ACHOT6_004453"
     CDS             complement(1159..2199)
                     /locus_tag="ACHOT6_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFP9949548.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     EVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRE
                     SLKVGKIGAVNKNSTENGYVSAQNRPKNRTLSCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPPVFLYGLSEPIGKFPMSDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELAAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGDLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            complement(2171..2722)
                     /locus_tag="ACHOT6_004454"
     CDS             complement(2171..2722)
                     /locus_tag="ACHOT6_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9949549.1"
                     /translation="MKITTEQVCEALDTWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKRDKFTALIPAIMEAIRISDFRLY
                     CEITDGKSITYMIAGLNKEYGDVVESGLLFADPVVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQMSARGSYEYRCTTMG"
     gene            complement(2706..2933)
                     /locus_tag="ACHOT6_004455"
     CDS             complement(2706..2933)
                     /locus_tag="ACHOT6_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9949550.1"
                     /translation="MLKIDAIAFFGSKTKLANVAGVRLASVAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHH"
     gene            3010..3417
                     /locus_tag="ACHOT6_004456"
     CDS             3010..3417
                     /locus_tag="ACHOT6_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949551.1"
                     /translation="MKNETFGARLLYRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPVNLTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            3682..3981
                     /locus_tag="ACHOT6_004457"
     CDS             3682..3981
                     /locus_tag="ACHOT6_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949552.1"
                     /translation="MALYIDISAIAGQVRVIRAVTKRYAPLLQKVSGECTEDIVNDFV
                     IELRGLIFSYKVTTIFADGSRETVRALRLKGCVKDLATTFWARKLDCIHNQFPLE"
     gene            4054..4272
                     /gene="ydfC"
                     /locus_tag="ACHOT6_004458"
     CDS             4054..4272
                     /gene="ydfC"
                     /locus_tag="ACHOT6_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFP9949553.1"
                     /translation="MQKRDPVIIAPDYTDDELYEWMHQKIKAVQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            4295..4702
                     /locus_tag="ACHOT6_004459"
     CDS             4295..4702
                     /locus_tag="ACHOT6_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949554.1"
                     /translation="MEFKDLPTPLQEMASNIVRSQLATLDLSTAEKETIDNMVRNVRN
                     AFSGLYGSDNQKQESDVNKRVISVCVNGHVLSSIKTETATVFDCLCIVQSLVDALFRS
                     VNLENDANLRGRIIAHPYAHTLGSVDIKDPTNL"
     gene            complement(4680..4913)
                     /locus_tag="ACHOT6_004460"
     CDS             complement(4680..4913)
                     /locus_tag="ACHOT6_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949555.1"
                     /translation="MLKSTLIAKCLYQNRMVSSISIGESAVKSIFEEYFPGHDFNKWN
                     TKLPPAVSTRILKATERASTIRVNYFIKDLWDL"
BASE COUNT         1339 a         1379 c         1214 g         1451 t
ORIGIN      
        1 gcggcgcaga ctcgctccca cttctggaca tgattcttcg ccctacgcag ttcgcgattt
       61 gccatatgca gcgatggcaa aatcaaatca tcttctcgcg ttgcagtaaa cgatggcagc
      121 gactgcacaa tgtccgccac agtttctgtt ttaatatctt cctgtgttgc atctttctgt
      181 accggtaacg caacaccggc tggctgagga aaggctttac catcggtttc cgctaccgat
      241 tcaactttcg gctctgctgg taaattatca cctggtatgc agtaacgaat tttaccgttc
      301 tggtttacgc gaatcagacg acctttgctg attgccattg ccagcgttga agcaactttg
      361 cgggatgttg taccgaacag cgtagccagt tcatccgccg tttgtggtcc gcgttgttca
      421 atcgtcgcgg ttaaatcgca ctctgagatt ttcgcttctg ttgccgtggt ggtttcttct
      481 ggcgctggct gttcctgctg aacgttgtta tcagccacac gccaggtgta tacgctttta
      541 tcaacgaagc cagccttttt cagttcccac agctcgttca gtacttcttc acgactgata
      601 tcaaggcgcg cagccagctc taccgacgtg gcttttccca tcgctttcag tgcgtcaaaa
      661 acagtctcca taaatttcct cccggtaaaa aatcacttat caactcaaac aaaaccagcc
      721 gctttccggc gttcatattc ctgtttcagc aactcaattg gcgttggccc cgacgggcgt
      781 ttgggggccg ccagttgtcg ccggactggc ggaacgctca ggccgttact aacatgcttt
      841 gcccatttcg tcagttgccg ttctgcaagc cgttttaatt ccccttcggt catctggcgc
      901 tcaatcccct ttgaacgcat ctcgaggcaa atgtgataca gcacaggctg agaccacggg
      961 tacttatcac ttccgtcgta tcgccaggac tcattgcgcc agcggcagta ctcctccatc
     1021 acagcatcca ccgtcagacc gaatggattg gctccgcttt tcgaaatcag tgccacaaac
     1081 tcagccaggt ccggaggcca tgtttcaccc gcccggcagc agtccatgca ctggcggcag
     1141 acctgccgga tttgctgctc agtcatcgcg ccaatctgtg caatccagag cttcgaaggt
     1201 gcggccccgt tcttctgggt ccagcggttc gaataaatca cccccatgag ttcccacagc
     1261 ttccaggccg tttccgtcgc tgataaatcc gttttcacgt tcccactgct cacgtgctgc
     1321 ccgaatttcc tgaactgccc gtgatgcggt gccacctggt gctgctgcat ggtttacccc
     1381 tttgctgact ggtttaacct gcgccctgac gtgatttacg tgacgggcga atttctgctc
     1441 ccactgaatc tgcgtaaaca ctttcccctc cgctgcccag tagtcccgga aggcggcaag
     1501 ttcagcaggt gtaaattctg tctccggcaa agccatcccc cacaacgcag cccgtcgtcg
     1561 aaaatcccgt gacggatacc agctatcgct catcgggaat tttccgatgg gttcgctcag
     1621 gccatacagg aatacagggg gggctgcctg taacgacaaa acttcctgct cactggtcgg
     1681 agcactctcg cgtgcgttat gtgtggggtt tagatctttg ggttcctttg ggttccgtga
     1741 tccgtttttg ggtgtctttg atggaaaatt tgggtgtctt tggttatttt ccatgcagct
     1801 aagagttctg tttttgggtc tgttttgtgc tgaaacataa ccattttcgg tactgttttt
     1861 attaacagca ccaattttac ccacctttaa agactcccgt ttttgggtgt attcaggctc
     1921 ggcaacactt tcttctacac cgataagtcg gtacaccaca atttgctttg ttctgccttt
     1981 tctctcaccg gtatcaacaa ttaacccaat ctccatcagg tgtcgtaagc tgtcctgcac
     2041 agtctttttg ttcagttccg ttacttctgc cagtgcagat acagacgggt atgcacacaa
     2101 atcggcaccg cacatatcag caagccaggt caatactgac ttactggatg aactgccggt
     2161 tttcaccttt ttagcccatc gtagtgcatc gatactcata cgaacccctg gcagacattt
     2221 gtttatctgc aaagtaatat tggtattgct gacgatacgc gtgcttgaaa gcaatagctt
     2281 tttctataag ctcgtcagtc tcacgttcca caacaactgg atccgcaaaa agcagcccgg
     2341 actccaccac atcgccatat tctttgttta acccggcgat catgtacgta atgctttttc
     2401 cgtcagtaat ttcacaatac aatctgaaat cgctgatccg gatagcctcc ataattgccg
     2461 gaatcagcgc cgtgaatttg tcacgcttat ctctggtgtc gatagctttc cagcgttcga
     2521 atatcttcac ccggttaacg cccagcgccc gttgatcaac ctcgccatca ttaaacgtga
     2581 cgcgttgaac atcgatgttc gggcgttctt tcagagccca gaatgcttcc gtgattaata
     2641 tcgtcgcctg ctcctgtgtc attcctggtc ggcataccca ggtatccaga gcctcacaaa
     2701 cctgttcagt ggtgattttc attgttcaac cgccccgccc gctttgcctt acgatattcg
     2761 tcataaactt tggggtcgta ctgaagttcc ccgccggatg cctcttgcag gcgcatcgcg
     2821 cgaccttcag gaaccagttc cccccatgca gcaacgcttg ccagcctaac tcctgcgaca
     2881 ttggcaagct ttgttttgct gccaaaaaac gctatagcat caattttcaa catatcgaac
     2941 cccttagatt ttcctaagga aactagatcg tagagaaacc taagtcaaga aaaattagaa
     3001 ttacctaata tgaaaaacga aaccttcggt gctcgcctct tatacaggcg aaaaaaatta
     3061 aaactgtctc aggccgcatt aggtaagctg gtcaaagtgg ctcacgtaac aatttctcaa
     3121 tgggaaagag atgaaacaca gccagcgggg aagagattat tcgcactgag ccaggcgctt
     3181 cagtgctcgc cgacttggct tctttttgga gatgaagata agcaaccagg cgaaccgatc
     3241 ccagataatc agccagtcaa tctgacagaa gatcaaaaag agttgcttca actgttcgac
     3301 gcactgcctg agtcagagca aaaggctctg ttgtcagaga tgcgtgctcg agttgagaat
     3361 ttcaacaaac tttttgaaga actactcaaa gctcgcaaaa gaagcgcaaa taaataaccc
     3421 cccttttttt cgcaactctc tgtaataaaa agcacaaact ttcaaatact tgtgtttttt
     3481 acatcaagaa gcttaggttt ttctacacaa aaagcttgac cataactctt aggctattct
     3541 aaattctact catcaagaca ccgcacggtg ttctcagcaa acagttccgc tacccggcgt
     3601 taaggggaag cagaggattt ctcagtgggc gaagtcaaac atcagaatgg aaggcgtcca
     3661 gggatcagca aagaaacagc gatggcgctt tatattgata tcagcgccat tgccggacag
     3721 gtaagagtta tcagagcggt aactaagcgg tatgcgcctt tacttcagaa agtctctggt
     3781 gagtgcaccg aagatattgt caacgatttc gtcatcgaac tgcgaggact catcttcagt
     3841 tacaaggtga ccacaatttt tgcagatggc tcccgcgaaa ctgtcagagc cctgcggctt
     3901 aaaggatgtg tcaaagactt agccaccaca ttctgggcaa gaaaacttga ttgtattcat
     3961 aaccaatttc ctctcgagta acagacccct cagaggatac cacctcgcct gacgtggtta
     4021 aaaagcaggc aacgctaacc acaaggagcc gacatgcaga aacgagaccc cgtcatcatc
     4081 gcgccagact ataccgatga tgaactttat gagtggatgc accagaaaat taaggcggtg
     4141 caggacctga aatgggccaa tgaagccagg gctaagcagg ctgaaaatct gtccgctctg
     4201 gagcaggata tcaccaatct ggaaaaagca gcggcattaa gcattgccag aatgattaca
     4261 tacccacgtt aatagctaac caacgaggct aataatggaa tttaaagatt taccaacgcc
     4321 attacaggaa atggcatcga atatagttcg ttcacaactg gctactcttg accttagtac
     4381 cgcagaaaaa gaaaccatcg ataatatggt tcgtaatgtg cgcaatgctt tttctgggct
     4441 atatggttct gataatcaaa agcaggaaag cgatgttaat aaacgggtaa tttctgtttg
     4501 cgtgaatggc catgttcttt catcaatcaa aacagaaacg gcgacagtct tcgattgcct
     4561 ttgcattgta cagagccttg ttgatgccct gtttcgttca gtgaatttag aaaatgatgc
     4621 aaatctgcga gggcgcataa tagcacatcc atatgcacat actttaggct ctgtggatat
     4681 caaagatccc acaaatcttt aatgaaatag ttaacgcgaa ttgtacttgc tctttcggtt
     4741 gctttcagaa tacgcgttga aactgctggc ggtaatttgg tattccattt attaaaatca
     4801 tgcccgggaa agtactcttc gaaaatactt ttaactgcag actcgcctat tgaaatgctg
     4861 cttaccatgc gattttgata aaggcattta gcaataagag ttgattttaa cattcaccct
     4921 cctgagggtt ggtaattaag gagttctcca cgggtgaagt ggagtgcgtg cgccggacac
     4981 gggtgaacat ccggcactga cagtttactg aaaggatatt tctctgaaaa gtcagagcat
     5041 aacgcgaaag cgcacggcga ggttgctggt tcatagatag cctgtcgtta aattttcgtc
     5101 gatcgtgcgc ttccggttgt ggcaatccgc gaaatggcgc ggcggtaagt atggcggggt
     5161 tattccttcc ccgttgagga caccgggttg tcaggttgac catacgctta agtgacaacc
     5221 ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcggtatc agttttactc
     5281 cgtgactgct ctgccgccct ttttaaagtg aattttgtga tgcggtgaat gcggctgagc
     5341 gcacgcggaa cagttaaaac caaaaacagt gttatgggtg gat
//
LOCUS       DAZKIV010000221         5357 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZKIV010000221 DAZKIV010000000
VERSION     DAZKIV010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5357
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.223"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..274
                     /locus_tag="ACHOT6_004461"
     CDS             <1..274
                     /locus_tag="ACHOT6_004461"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949556.1"
                     /translation="QYGSDESLTPHQQVTDNLRRCEMHKTEPKIVAPGYTNEEIYEWM
                     AKKLAAINQLREVLSYRQETIDSLKKLDQEITVLSQDVTLDIVQTN"
     gene            complement(246..680)
                     /locus_tag="ACHOT6_004462"
     CDS             complement(246..680)
                     /locus_tag="ACHOT6_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000016394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949557.1"
                     /translation="MSGNIYTLYKSHCENVGKYRGIEISGVVSSVEISKVESRATLLT
                     LLDLVLHEHRKKFGTPYNQLNGKKALVHLILMKHHWMPKQINEMKFDELLLSIQDELT
                     LDKISVTAQKFLDYRDWRSQIHHFDDFDENEWDPNLSAQYLK"
     gene            824..1146
                     /locus_tag="ACHOT6_004463"
                     /pseudo
     CDS             824..1146
                     /locus_tag="ACHOT6_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1225..1761
                     /gene="yfdR"
                     /locus_tag="ACHOT6_004464"
     CDS             1225..1761
                     /gene="yfdR"
                     /locus_tag="ACHOT6_004464"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9949558.1"
                     /translation="MSFIKTFSGKHFYYDKINKNDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPQSPGHA
                     YGMFMERFNELSELRKCA"
     gene            2723..3742
                     /locus_tag="ACHOT6_004465"
     CDS             2723..3742
                     /locus_tag="ACHOT6_004465"
                     /inference="COORDINATES: protein motif:HMM:NF025638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4365 domain-containing protein"
                     /protein_id="HFP9949559.1"
                     /translation="MTSESNRIGKMGVNYIEGVLTRWGWGYQSISQENDDGFDGLIYI
                     RSKRFEAKDKENRSKQSWEFTGGLIHVQVKSGDTYISSRNKDEIRIKIKNLNEKKKIW
                     SRSSIPCILVFVFWDHNEVEYSYWADLKSELTYCDNSSTLIKIPLKNRFMRSQECKGP
                     LRRLARVSANYADKPLIDLSKFDSLSGKLPTSLNSNLNCSLKKKGIEFYQKWRLIGAT
                     NPYFGPVLINRTGWSHITRRNRPIARIEASFNLLPLAARIINDVSTWRMLTPVRRYDN
                     RKDGYVCFIDFVGLTAKVNLKNRNSSEVMVVLKRERRYKQGEDCSSAKTRLWFYTVYE
                     PGRGK"
     gene            complement(4014..5237)
                     /locus_tag="ACHOT6_004466"
     CDS             complement(4014..5237)
                     /locus_tag="ACHOT6_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9949560.1"
                     /translation="MALSDAWLRSVVGKERDKVLVKSDRDGLSVRVSPKGRVVFQYRY
                     QWAGKGERLDIGTYPSTGLKEAREEVIRLRGELESNRNPRLVKQAEKRKATEAMTVES
                     VIRAWYEAYCVKNKKGSEQILRSFELHLFSKIGSIPHDAATLHDWLEVLEPLSTKTPA
                     IADRLLINAKQAHVWAYKRKLIETRPLSDITGKDMDIRKGQKKRFLTHDEIKILYAAI
                     DGSRMVPKYRAFIKLLLHFGCRSSELITARVDDFDFINKVWTVPPERHKTGDITGEPL
                     KRPIIEPVEELIKYVISMNNGSDMLFTKEGSREPVGRTSLQSLPYNLMQYAWRRLGYQ
                     FPHWSLHDLRRTARTNFSDLTAPHIAEIMLGHKLPGVWQVYDKSDYLEEQRKAYQAWW
                     ERVESIITCTISDSN"
BASE COUNT         1586 a         1146 c         1102 g         1523 t
ORIGIN      
        1 tcaatacggt tctgacgagt cgcttactcc ccatcaacaa gtaactgata acttgaggag
       61 gtgtgaaatg cacaaaacag aacccaaaat cgtcgcgccc ggatacacaa atgaggaaat
      121 ttacgagtgg atggcaaaga agctggcagc tataaaccag cttcgtgaag tgctgtctta
      181 tcgacaggaa acaatagact ccttaaaaaa actggatcag gaaatcacgg ttttatcaca
      241 ggatgttact ttagatattg tgcagacaaa ttaggatccc attcattttc gtcaaaatca
      301 tcaaagtgat gaatttgtga tctccagtct cgataatcta aaaatttctg ggcggttacg
      361 cttattttat caagtgtgag ttcatcctga attgaaagaa gaagttcatc aaatttcatc
      421 tcattaatct gttttggcat ccagtgatgc ttcatcagaa taaggtgaac cagagccttt
      481 ttcccattca actgattata gggagtgccg aatttcttcc ggtgctcatg taagacaagg
      541 tccagaagag taagtaatgt tgcccttgat tccactttgc ttatttcgac tgatgacact
      601 accccactga tttcaatgcc ccgatacttt ccaacatttt cacagtggga tttgtacagc
      661 gtatagatat taccggacat ttcttttcct tttgcgttgt tggggataac cagattaacc
      721 gaatccttgt tgttggggaa taaccaggtc cacctcgcct gatgtggcta aaagcaggca
      781 cataacagct aagtattttc aaccagagag aatccttagc gttgtggtga atgcggctca
      841 gcgcacgcgg gttaaggttg aggctgacag tcgaccttct gtggataccc acccgtctgg
      901 tgtacaacct tcgccaggca ccgggaggca cccggcacca caactttatg ctgtgtgtag
      961 tcttggcggt accagcttgt acccttgctt ccggctggta ccgtcctttt tacaaaacag
     1021 agaagagcat catcggacga cgggctcata acccaatcca tccgggctgc tgccaccgca
     1081 ggtgttcttc tctgttttgt ggagaaacta accgcccctg cggggcattt attgaaacgt
     1141 aattgactca ataatcgccg gatggagagg gcttcctttt accaaaattc agcgcggtgc
     1201 agcgcattta aagtggagaa cgaaatgtca tttattaaaa ctttttccgg gaagcatttt
     1261 tattatgaca agataaataa aaatgacatc gttattaacg atatcgcggt ttccctttca
     1321 aatatctgtc gctttgctgg tcatctttca cacttctaca gcgttgccca acatgcggtg
     1381 ctttgcagcc agctggtacc acaggaattt gcttttgaag cgttaatgca tgatgcaaca
     1441 gaagcgtatt gtcaggacat ccccgcgcca ctgaaacgcc ttcttcctga ctataaacgg
     1501 atggaagaaa aaatagacgc cgtaatccgt gagaaatacg ggttaccccc ggttatgagc
     1561 acgcctgtaa aatatgccga tctcatcatg ctcgcaaccg aacgccgtga tctcgggctt
     1621 gatgatggct ctttctggcc tgtactggaa ggtatcccgg caacagagat gttcaaagtt
     1681 attccacagt cgccaggcca tgcctacggg atgtttatgg aacgctttaa cgagttatcg
     1741 gagttacgca aatgcgcatg agtctttcaa ctgcacaacc attggagggg tagcaatgaa
     1801 caacttaatg acaactaaac aggtcgccga cttctgtggt gtttcaatat caacagtgct
     1861 tcgctggaac agcataaacc atagaacagg taagaaatat agaccagact ttcctgatcc
     1921 tgatataaaa tcctgtccca ataaatgggc atcacacaag atttataggt ttgcgggtat
     1981 tatcgaataa cgtttatttg tttcgatgtt agctgacaca tctatggcac agagcaaaac
     2041 cgaatatgat tgtccactct gtgctagtag aaaacataga tatcaactat ccgtaccaat
     2101 ctgtgagtat agaataaatt aatctgctcg caactaatag cctctaacag ccaatacagt
     2161 gccaccgatg atacaaaaaa caatactcca aaaagcgtct ggtattggat tgtcattact
     2221 agcttccgag gtgagatatg cattaagcca ggtgcgctgg aaacgttatg tttccctaat
     2281 ggaattgccc atgactcaac atattttttc tctgttgcta gcgaatagac ctaaaatgat
     2341 aagcaccatt aacatcaaca gcgccccctt gagctcttct tttcggtgaa aagcccatca
     2401 tcaacggcgc attgcctaac ccgaattcta gccaccttag ccagtatttc ccccctagat
     2461 accaaatgct tacccctcta tttgctctga taatacttta ttgcataatc cctcactaca
     2521 ttagacactg cgtcaatcct gccttctata gcttgtttta acgctttcgg cacattcctc
     2581 tgaaccagcc ccagatatgg ctgttaaatt tttacttaca ttcaaaaaaa ctttaacttt
     2641 tgcaatcaga tcatatgatg ttgttaatgg taatattctg aatctaaaat gacttaagag
     2701 ttcgtaggtt agagggacat gtatgacttc tgaaagcaac agaattggga agatgggtgt
     2761 aaactacata gaaggtgtat tgacaagatg gggatgggga tatcaatcaa tttcgcaaga
     2821 aaatgatgat gggtttgatg gcttaatata tataagatct aaacgatttg aggcgaaaga
     2881 taaagaaaac cggagtaaac aatcttggga gttcactggt gggttgattc atgtacaagt
     2941 aaaaagtgga gatacataca ttagtagtcg taataaagat gaaataagga tcaaaataaa
     3001 aaacctcaat gaaaagaaga aaatatggtc tagatcgtct attccatgca tacttgtatt
     3061 tgttttttgg gatcataatg aagttgagta ttcctactgg gctgatctta aatctgaatt
     3121 aacttattgt gataacagta gtacgctaat aaaaatcccc ttaaaaaata gatttatgag
     3181 atcccaagag tgtaaggggc ctttaagacg acttgcaagg gtatcggcca actatgcaga
     3241 taagccactg atagatttat caaaatttga ctcattaagt ggtaagctac ctacaagtct
     3301 aaacagtaac ctaaattgtt ctttgaaaaa gaaaggtatt gaattctatc agaaatggcg
     3361 attaattggg gctacaaacc cttattttgg tcctgttcta ataaacagaa ctggttggag
     3421 tcatataact cgacgaaatc gcccaatagc aagaattgaa gcaagcttta atttgttacc
     3481 tttagcagca agaattataa atgatgtatc tacatggcga atgttaacac ccgtacgacg
     3541 gtatgataat agaaaggatg gttatgtttg ctttatagat ttcgttgggt taacagccaa
     3601 agtgaattta aagaatcgca actcatcaga agttatggtt gtgttaaaaa gggagaggag
     3661 gtataaacag ggtgaagact gttcctctgc gaaaacaaga ttatggtttt acactgttta
     3721 tgaacctgga agaggaaaat aaattgaggc ggtacaatcg cgctcaggct gtcccagacg
     3781 tgatgcataa caccaccggt tccagaattt aaggatctgg accttcaatt atgcgtgtac
     3841 cgccttatgc aacaatacta taaaattatt aaacgtcaag atgttcaata agatttgggc
     3901 tttcatgcca caacacgaaa gttcatttta taacgtctaa agctttcaca atgtccgctt
     3961 ttcgcagctc aaagcggaca gtcatatttg attgtattct ggctactgga gcctcagtta
     4021 gagtctgaga tagtacaagt aatgatcgat tcaactctct cccaccatgc ctggtaggct
     4081 ttacgctgtt cttctagata atcgctctta tcataaactt gccatacccc tggcagttta
     4141 tggccgagca ttatttctgc aatatgaggc gcagtaagat cagaaaagtt tgttcgtgct
     4201 gttcgcctca aatcatgaag agaccaatga gggaattgat accccaaacg ccgccatgcg
     4261 tactgcatta aattgtaagg cagcgactgc aatgatgttc gtccaacggg ttccctgctt
     4321 ccttccttag taaaaagcat atcggaacca ttgttcatag agataacgta ctttataagc
     4381 tcttcaaccg gttcaataat ggggcgcttt agcggttcgc ctgttatatc ccctgtctta
     4441 tgtcgttctg gcggtacagt ccatacctta ttaatgaaat caaaatcatc caccctagcg
     4501 gtaattagct ctgaactacg gcagccaaaa tgcagcaata gtttaatgaa ggcccggtat
     4561 ttaggaacca ttcgagaacc atcgatcgca gcataaagga ttttaatttc atcatgtgtc
     4621 agaaaccgtt tcttctgacc tttacggata tccatatctt tacccgtgat atccgacagc
     4681 gggcgggttt caatgagctt tctcttatag gcccagacat gggcctgctt tgcgttaatt
     4741 agcaatcggt ctgctattgc tggagtctta gtgctaagag gctctaggac ttctaaccaa
     4801 tcatgcaatg tagctgcatc gtgagggata ctcccgattt tagagaacag gtgcagctca
     4861 aacgagcgga gtatctgttc agaacctttt ttatttttta cacaatatgc ttcataccag
     4921 gcacggatca cagactctac cgtcatggct tcagtagctt ttcgtttttc agcctgcttg
     4981 accaatcgtg gattacggtt tgactcaagt tcgccacgga gacggataac ttcttctctg
     5041 gcctctttta atccagttga cgggtaagtt ccgatatcaa ggcgctcacc tttccctgcc
     5101 cattgataac gatattggaa cactacgcga cctttcggtg atactctgac agacagacca
     5161 tcacgatcgg atttaaccaa aaccttatca cgttcctttc caacgactga acgcaaccac
     5221 gcatcagaca gcgccattac tcaccttgta caaatcgcaa aacacctctg ctgctttgta
     5281 cattatgtac aagcatatgt acagattttt gatgaaagca acatgatcga tttaaagtta
     5341 catgaaagaa ttttcaa
//
LOCUS       DAZKIV010000222         5276 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZKIV010000222 DAZKIV010000000
VERSION     DAZKIV010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5276
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.224"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..1110
                     /gene="glaH"
                     /locus_tag="ACHOT6_004467"
     CDS             133..1110
                     /gene="glaH"
                     /locus_tag="ACHOT6_004467"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFP9949561.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDFDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1130..2398
                     /gene="lhgO"
                     /locus_tag="ACHOT6_004468"
     CDS             1130..2398
                     /gene="lhgO"
                     /locus_tag="ACHOT6_004468"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFP9949562.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPRPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2421..3869
                     /gene="gabD"
                     /locus_tag="ACHOT6_004469"
     CDS             2421..3869
                     /gene="gabD"
                     /locus_tag="ACHOT6_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFP9949563.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            3883..5163
                     /gene="gabT"
                     /locus_tag="ACHOT6_004470"
     CDS             3883..5163
                     /gene="gabT"
                     /locus_tag="ACHOT6_004470"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFP9949564.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLVIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
BASE COUNT         1205 a         1428 c         1540 g         1103 t
ORIGIN      
        1 tgcttttgat cacaataaga aaacaatatg tcgcttttgt gcgcattttt cagaaatgta
       61 gatattttta gattatggct acgaaatgag catcgccatg tcaccctaca tctcataaga
      121 ggatcgcttc tgatgaatgc actgaccgcc gtacacaata acgctgtcga ttcaggccag
      181 gactatagcg gattcactct catcccgtcg gcgcaatccc cgcgtctgct ggaactcacc
      241 ttcaccgaac agacgaccaa acagtttctc gaacaggttg ccgaatggcc cgtgcaggcg
      301 ctggagtaca aatcgtttct gcgttttcgg gtaggcaaaa ttcttgacga tctgtgtgcg
      361 aatcagctgc aaccattgct gttgaagacc ctgctaaacc gtgctgaagg tgcgctgttg
      421 atcaatgcgg tgggtatcga tgatgtggcg caggcagatg agatggtgaa gctggcaacg
      481 gcggtagcgc atctgattgg tcgctcaaat ttcgatgcga tgagcggtca gtattacgcg
      541 cgtttcgtgg tgaaaaatgt cgataactca gacagctatc tgcgtcagcc gcaccgcgtg
      601 atggaactgc acaacgacgg cacgtatgtc gaagagatca ccgattacgt gctgatgatg
      661 aaaatcgatg agcaaaacat gcagggtgga aattcgttgc tgctgcatct cgatgactgg
      721 gaacatctgg accactattt ccgccatcca ctggcgcgtc gcccaatgcg ctttgccgcg
      781 ccgccgagca aaaacgtcag caaagatgtt ttccatccag tgttcgactt tgatcaacag
      841 ggtcgcccgg tgatgcgcta tatcgaccag ttcgtccagc cgaaagactt cgaagaaggc
      901 gtgtggttga gcgagctttc ggacgccatt gaaaccagca aaggcattct ttctgtaccc
      961 gttcccgttg gcaaattcct gttgataaac aacctgttct ggctgcacgg tcgcgaccgc
     1021 tttactccgc acccggatct gcgccgtgaa ctgatgcgtc agcgcggcta cttcgcttac
     1081 gccactcacc actaccagac gcatcagtaa gcgcaaagga attgagcgga tgtatgattt
     1141 tgtgattatt ggcggcggca tcatcggcat gtcgaccgcc atgcaactga ttgatgtcta
     1201 tccggatgcc cgcattgcgt tgctggaaaa agagtccggc ccggcctgtc accagacggg
     1261 ccacaacagc ggcgtgatcc atgccggggt ctattacacg cccggcagcc tgaaggcgca
     1321 gttttgcctg gcgggaaacc gcgccaccaa agccttttgc gatcaaaacg acattcgcta
     1381 tgacaactgc ggcaagatgc tggtcgccac gtccgatctc gaaatggaac ggatgcgcgc
     1441 gttgtgggaa cgcacggcgg cgaacggtat cgagcgcgag tggttaaacg ccgaggaact
     1501 gcgcgagcgc gaaccgaata tcaccgggct cggcggcatt tttgtgccgt ccagcggcat
     1561 tgtcagctat cgcgaagtca cagcggcaat ggcaaaaatc ttccaggcca gaggcggtga
     1621 gattatttat aacgccgaag tcagcgccct cagtgagcat aaaaacggcg tggtcgtccg
     1681 aacccgtcag ggcggcgaat atgaagcatc gacgctgatt agctgttccg ggctgatggc
     1741 tgaccggctg gtgaaaatgc tcggcctcga accgggtttt attatctgcc cgttccgtgg
     1801 cgagtatttc cgccttgcgc cggagcataa ccagattgtt aaccatctga tttaccccat
     1861 tcccgacccg gcaatgccat ttttgggcgt gcatctcacc cgaatgatcg atggcagcgt
     1921 caccgtcggg ccaaacgcgg tgctggcttt caaacgcgaa ggctatcgca agcgcgactt
     1981 ctcatttagc gacacgctgg agattttggg ctcgtcgggg attcgccggg tgctgcaaaa
     2041 ccatctacgc tcaggactgg gcgagatgaa aaactcgctg tgcaaaagcg gctatctgcg
     2101 cctggtgcaa aagtattgtc cccggctttc gttaagcgat ctccagccca ggcccgccgg
     2161 tgtgcgggcg caggcagtat cgccggacgg caagctgatt gacgattttc tgtttgtcac
     2221 caccccacgc acgatccaca cctgcaatgc gccctccccg gcagcgacat cagcaattcc
     2281 tattggtgcg catattgtca gcaaggtaca aacgctgttg gcaagccaga gtaaccccgg
     2341 acgcacgctg cgagcggcac gtagtgtgga tgccttacac gccgcattta atcaataacc
     2401 tttgaaaaca ggacgtaaca atgaaactta acgacagtaa cttattccgc cagcaggcgt
     2461 tgattaacgg ggaatggctg gacgccaaca atggtgaagt catcgacgtc accaatccgg
     2521 cgaacggcga caagctgggt agcgtgccga aaatgggcgc tgatgaaacc cgcgccgcta
     2581 tcgacgccgc caaccgcgcc ctgcccgcct ggcgtgcgct caccgccaaa gaacgcgcca
     2641 acattctgcg caactggttc aatttgatga tggagcatca ggacgattta gcgcgcctga
     2701 tgaccctaga acagggcaaa ccactggccg aagcgaaagg tgaaatcagc tacgccgcct
     2761 cttttattga gtggtttgcc gaagaaggca aacgcattta tggcgacacc attcccggtc
     2821 atcaggccga taaacgcctg attgttatca agcagccgat tggcgttacc gcagctatca
     2881 cgccgtggaa cttcccggcg gcgatgatta cccgtaaagc cggtccggcg ctggcggcag
     2941 gctgcaccat ggtgctgaag cccgccagtc agacaccgtt ctctgcgctg gcgctggcgg
     3001 agctggcgat tcgcgcgggc attccggctg gcgtgtttaa cgtggtcacc ggttcggcag
     3061 gcgcggtcgg taatgaactg accagcaacc cgctggtacg caaactgtcg tttaccggtt
     3121 cgaccgaaat tggccgccag ttaatggaac agtgcgcgaa agacatcaaa aaagtgtcgc
     3181 tggagctggg cggcaacgcg ccgtttatcg tctttgacga tgccgatctc gataaagccg
     3241 tggaaggcgc gctggcctcg aaattccgca acgccgggca aacctgcgtc tgcgccaacc
     3301 gtttatacgt gcaggacggc gtgtatgacc gctttgccga aaaattgcag caggcggtga
     3361 gcaaactgca catcggcgac gggctggata aaggcgtcac catcgggccg ctgatcgatg
     3421 aaaaagcggt agcaaaagtg gaagagcata ttgccgatgc gctggagaaa ggtgcgcgtg
     3481 tggtttgcgg cggtaaagcg gatgaacgcg gcggcaactt cttccagccg accattctgg
     3541 tggacgtgcc ggccaacgcc aaagtgtcga aagaagagac gttcggcccc ctcgccccgc
     3601 tgttccgctt taaagatgaa gccgatgtga ttgcgcaagc caatgacacc gagtttggcc
     3661 ttgccgccta tttctacgcc cgtgatttaa gccgcgtctt ccgcgtgggc gaagcgctgg
     3721 agtacggcat cgtcggcatc aataccggca ttatttccaa tgaagtggcc ccgttcggcg
     3781 gcatcaaagc ctcgggtctg ggtcgtgaag gttcgaagta tggcatcgaa gattacttag
     3841 aaatcaaata tatgtgcatc ggtctttaac tggagaatgc gaatgaacag caataaagag
     3901 ttaatgcagc gccgcagtca ggcgattccc cgtggcgttg ggcaaattca cccgattttc
     3961 gctgaccgcg cggaaaactg ccgggtgtgg gacgttgaag gccgtgagta tcttgatttc
     4021 gcgggcggga ttgcggtgct caataccggg cacctgcatc cgaaggtggt ggccgcggtg
     4081 gaagcgcagt tgaaaaaact gtcgcacacc tgcttccagg tgctggctta cgagccgtat
     4141 ctggagctgt gcgagattat gaatcagaag gtgccgggcg atttcgccaa gaaaacgctg
     4201 ctggttacga ccggttccga agcggtggaa aacgcggtaa aaatcgcccg cgccgccacc
     4261 aaacgtagcg gcaccatcgc ttttagcggc gcgtatcacg ggcgcacgca ttacacgctg
     4321 gcgctgaccg gcaaggtgaa tccgtactct gcgggcatgg ggctgatgcc gggtcatgtt
     4381 tatcgcgcgc tttatccttg cccgctgcac ggcataagcg aggatgacgc tatcgccagc
     4441 atccaccgga tcttcaaaaa tgatgccgcg ccggaagata tcgccgccat cgtgattgag
     4501 ccggttcagg gcgaaggcgg tttctacgcc gcgtcgccag cctttatgca gcgtttacgc
     4561 gcgctgtgtg acgagcacgg gatcatgctg attgccgatg aagtgcagag cggcgcgggg
     4621 cgtaccggca cgctgtttgc gatggagcag atgggcgttg cgccggatct caccaccttt
     4681 gcgaaatcga tcgcgggcgg cttcccgctg gcgggcgtca ccgggcgcgc ggaagtgatg
     4741 gatgccgtcg ctccaggcgg tctgggcggc acctatgccg gtaacccgat tgcctgcgtg
     4801 gcagcgctgg aagtgttgaa ggtgttcgag caggaaaatc tgctgcaaaa agccaacgat
     4861 ctggggcaga agttgaaaga cggattgctg gtgatagccg aaaaacaccc ggagatcggc
     4921 gacgtacgcg ggctgggagc gatgattgcc atcgagctgt ttgaagatgg cgatcacagc
     4981 aaaccggacg caaaactcac cgccgagatc gtggcacgcg cccgcgataa aggcctgatt
     5041 cttctctcct gcggcccgta ttacaacgtg ctgcgcatcc ttgtaccgct caccattgaa
     5101 gacgctcaga tccgtcaggg tctggagatc atcagccagt gttttgctga ggcgaagcag
     5161 tagctatgtc cgtttgtaag agttaaacgt tgctaaggga atgataacga tgcagtaaaa
     5221 aataataacg atgaggcgat cgacaaagtt aaaaacgact gcggactgtc ccctcg
//
LOCUS       DAZKIV010000223         5155 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZKIV010000223 DAZKIV010000000
VERSION     DAZKIV010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.225"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(464..1096)
                     /gene="yfdX"
                     /locus_tag="ACHOT6_004471"
     CDS             complement(464..1096)
                     /gene="yfdX"
                     /locus_tag="ACHOT6_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFP9949565.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            1392..1667
                     /gene="ypdI"
                     /locus_tag="ACHOT6_004472"
     CDS             1392..1667
                     /gene="ypdI"
                     /locus_tag="ACHOT6_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HFP9949566.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(1744..1986)
                     /gene="yfdY"
                     /locus_tag="ACHOT6_004473"
     CDS             complement(1744..1986)
                     /gene="yfdY"
                     /locus_tag="ACHOT6_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFP9949567.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            2339..3259
                     /gene="lpxP"
                     /locus_tag="ACHOT6_004474"
     CDS             2339..3259
                     /gene="lpxP"
                     /locus_tag="ACHOT6_004474"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFP9949568.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            3615..3686
                     /gene="ypdK"
                     /locus_tag="ACHOT6_004475"
     CDS             3615..3686
                     /gene="ypdK"
                     /locus_tag="ACHOT6_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFP9949569.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(3751..4989)
                     /gene="alaC"
                     /locus_tag="ACHOT6_004476"
     CDS             complement(3751..4989)
                     /gene="alaC"
                     /locus_tag="ACHOT6_004476"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFP9949570.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
BASE COUNT         1355 a         1234 c         1162 g         1404 t
ORIGIN      
        1 aggcgtaata tcttctataa ataacccgca aaatatcgtc gtagctgcac tcgatatttt
       61 taaaatcctg tatcaagtgc tgaaaatcat ttcatcgcca cttccaattc ctttctgcat
      121 ggtaagtagt gaggacgaaa agaagaatat gataaatatt gattcgtggt aatcagagag
      181 agtcgcgaca tttgtgatgt tattcttaca actgtaagaa gcgccggtga tttacaagta
      241 agggtatgat tacgcttttt aaagcgtacc ataaaacata acataataaa tgatttgtga
      301 tgacacggga ttattgggat ttactttgag gaagataaaa tgcgtgttta tagaaccgcg
      361 agcgccaaat aattttatga ccacaacctg tcgtattgtg gtcataaaaa attgtcggct
      421 ggagggagtt aaatccttta cctcaatata ctgcctgtca gcattagttc acaaacagtg
      481 cttcgctgtc gacgatgatt ccatcttccg cacctttaag cgccaggttt gcctcgtagt
      541 attgcttttt atcaagcagt ttctgcgcat cagcaagtgc attacgagtc tgtttaagcg
      601 gcatcagata ttggttttcc attacgccaa caccggccag gcgaagttct tccattgcgc
      661 cctttttatc ccctttcgcc attttttcat tggcaatttt tatcgcggct tctttttcgg
      721 gagttgctac ataactctca gaaattccga cagatgcatt aatgacaata tattggtcat
      781 cattgagatt ggtcttttta cctggcttag cgaactttgc ccattcggta ctgtcatctg
      841 acagcaaagc ggaagcttca ttggtcagtt cttttgcctt ttctggatcg ccatgaaaca
      901 gagccaggcg cgctacctgc acgtcacgca tcgcatataa accttgttca gagatacgct
      961 ctgctgctgc cgttttctgg gtttgctgaa cttgctgagt ttgctgttgt gccgctaccg
     1021 gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc atcgtagcca
     1081 taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata
     1141 ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac
     1201 agttgtagtt attatcgcaa gaagtatttt agtcttacaa ttgtaggaat ctgcttcctg
     1261 tcgcagagaa ctattcttat gtaccacaaa gagatgtcat ttattatatc gatgtaaata
     1321 ttgattaatt atatgtaaat acagccataa catcgtaggt tctcagtgaa ccattttcag
     1381 gataactttc tatgaaagta aacttaatac ttttcagctt atttttattg gtctctatta
     1441 tggcatgcaa tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag
     1501 agactgaaaa aacatcagcg atgacgacca cacactctac aaaacttcag ccatcacagg
     1561 cgattttgtt taagatgaga gaagatgcgc caccattaaa cctcacagaa gaaatcacgc
     1621 ctacttatcc gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg
     1681 gcaggtgaac aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac
     1741 ccactatcgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag
     1801 aaacataaat atccccgtca ccagttcatt accgccagtt aaccacatgg tgaagtaata
     1861 aatgagtgca gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag
     1921 tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata
     1981 aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta
     2041 agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc atgttaaatg
     2101 caagactgtt gtgtacggaa aaatatttac tttgcacgat taataatcag tagctgaaag
     2161 cagtcagcga gtgataaagt acagataccg cattcatgct cctcgtggtt atgtcctgac
     2221 tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt
     2281 accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat
     2341 gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt
     2401 tgggcttggt gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg
     2461 cacgcgtatt ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa
     2521 aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga
     2581 aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga
     2641 cagtcgcgta cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat
     2701 gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg
     2761 cgtgatggga ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat
     2821 ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa
     2881 tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca
     2941 ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac
     3001 aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat
     3061 ggtaagaaaa gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta
     3121 cccgacagat gaaaatcaag ccgctgccta tatgaacaag attatcgaga aagagatcat
     3181 gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga
     3241 atcgtcgttg tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat
     3301 aacctcatgg tttttatggc ttttttctta aggttttttc cttcgctgaa tatctaatta
     3361 ttaccgtcag aaattaaact gtcgccgata tgagacactt gtaagttact aaaagtaaat
     3421 aagaaaatac ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt
     3481 ttagctcaga attctggggt gatgcccacg gtaaaataca gggcttttaa ggtcgctttg
     3541 cccggtgtca actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg
     3601 cggagagatg taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg
     3661 ccggtacttt tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca
     3721 ggctcctgtt tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct
     3781 gctggcgggt agtaaaccgt cggcacggaa catcgcttta atccctctga tggcctgacg
     3841 aatacggtcg cggttttcaa tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa
     3901 gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg caaattccag
     3961 cgatcccatg gccgcatatg gttccgggat tttcgcccag acatacatcg aagccttcgg
     4021 catttcgacc atccagcccg cttcatgcag ccctttaacc aatacatcac ggcggcgttt
     4081 gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc
     4141 cacctgcaac ggcgtaaaag tgccgtaatc gtgatagctt ttgattcgcg ccagggcact
     4201 gaccagcgtt ttgttgccga ccataaagcc gatacgccag cccgccatgt tgtagctttt
     4261 cgacagcgta aagaactcga ctgccacatc gcgtgcaccc ggtacctgca taattgacgg
     4321 cgctttccag ccatcgtaga cgatatcggc ataggccagg tcatggacca ccagcacatc
     4381 gtagcgtttc gccagcgcca ctactttttc gaagaactcc agttccacgc attgcgcggt
     4441 tgggtttgac gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc
     4501 gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgcgc
     4561 cccggcaatc accgcgccat aaatatggat cgggtaactt ggattcggca ccagcaccgt
     4621 gtcaccatga tccagcgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt
     4681 gacgatggct tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga
     4741 aatggcgcga cgtaagcgcg gaatgccgcg tgacgtggag taaccgtgcg tgtccgggcg
     4801 ctgggccaca gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc
     4861 catactgaaa tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt
     4921 gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt
     4981 gtcagccata atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac
     5041 aatggtggct gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat
     5101 ttttatcaat ggctttaaat gaggaactcc acttcaaaaa caggaaattt ttcta
//
LOCUS       DAZKIV010000224         5110 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZKIV010000224 DAZKIV010000000
VERSION     DAZKIV010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5110
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.226"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..868
                     /locus_tag="ACHOT6_004477"
                     /pseudo
     CDS             1..868
                     /locus_tag="ACHOT6_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(896..985)
                     /locus_tag="ACHOT6_004478"
     tRNA            complement(896..985)
                     /locus_tag="ACHOT6_004478"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(949..951),aa:Ser,seq:cga)
     gene            1079..1876
                     /gene="mtfA"
                     /locus_tag="ACHOT6_004479"
     CDS             1079..1876
                     /gene="mtfA"
                     /locus_tag="ACHOT6_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFP9949571.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            1977..2052
                     /locus_tag="ACHOT6_004480"
     tRNA            1977..2052
                     /locus_tag="ACHOT6_004480"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2010..2012,aa:Asn,seq:gtt)
     gene            2366..>5110
                     /gene="yeeJ"
                     /locus_tag="ACHOT6_004481"
     CDS             2366..>5110
                     /gene="yeeJ"
                     /locus_tag="ACHOT6_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HFP9949572.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQFAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSENNLTPPPGNSSGNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAEGWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSEWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISISSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVELQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVKVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     V"
BASE COUNT         1438 a         1199 c         1229 g         1244 t
ORIGIN      
        1 acattcggtc gcttgtggga aaaattcctc gccagcgcct attacagtga cctcagtcca
       61 agaacacaaa aatattatct acaacatcaa aaaaagttgc ttgccgtatt cggtaaggtg
      121 ccggcagatt ccataaaacc agaacacatc cgtcgataca tggacaagag aggggagcag
      181 agtaaaacgc aagccaacca tgaaaaaagc agtatgtccc gcgtttacag ttgggggtat
      241 gagcgagggt acgtgaaggg taacccatgt gcaggtgtaa gtaaattcaa ggccaaaaac
      301 cgcgaacgat atgtaaccga caaagaatac caggcagtat taagcgttgc acctcttcct
      361 gtttttatcg caatggaaat tgcctatctg tgtgcagcga gggtttccga tgtgttatcg
      421 ctgaaatggg agcagattgg aaacgacgga atctttatcc agcaagggaa aacaggaaaa
      481 aaacagataa aagcatggag tccacgatta caggcggcga tcgaaaaagc aaaacagtta
      541 ccaacatccg cctatgtaat cagtaatcaa tacggcaacc gatatatgta caaaggcttt
      601 aacgaaatgt gggtagaagc aagaaatcgc gcaggcaaaa tttcaggtat tttaaccgac
      661 ttcacctttc atgatctgaa ggcgaaagga atttcagact atgaaggaag cagtcgggat
      721 aagcaacttt tctctggtca caaaaccgag gggcaagtgc taatctatga caggaaggtt
      781 aaagtttcac cgacacttga tgtcccgtta cctgaaaata ttccaagaaa atattccaag
      841 taattccaag tgtgattttt gtcactgact taatgatgta taagtgattg aattttggcg
      901 gagagagggg gatttgaacc cccggtggag ttgcccccac tccggttttc gagaccggtc
      961 cgttcagccg ctccggcatc tctccgttca gatggttgcc atgatgccag gaaatttggc
     1021 attttaacag tccctgtccg tgcaattttg ttcaagtgac gagtttgcga gcaaaacgat
     1081 gattaagtgg ccctggaaag tacaagaatc agcacatcaa actgcccttc cctggcagga
     1141 agcactatcg atcccccttt taacgtgtct gacggaacag gaacaaagca aattagtcac
     1201 tcttgccgaa cgttttttac agcaaaagcg gcttgttcct ttacagggct ttgaactgga
     1261 ttcattaaga agctgccgga tagcacttct attttgccta cccgttctgg agttaggact
     1321 ggaatggctg gatggttttc atgaagtctt aatttatcct gcgccatttg tggtcgatga
     1381 tgaatgggaa gacgatatcg gtctggtgca taaccaacgt attgttcagt caggtcagag
     1441 ctggcagcaa gggcctatcg ttttgaactg gttggatata caagattctt ttgatgcatc
     1501 tggttttaac ctgattattc atgaagtcgc tcataagctg gacacccgta acggcgatcg
     1561 cgccagcgga gttcccttta ttccgttgcg tgaggttgct ggctgggaac acgatcttca
     1621 tgctgcaatg aacaacattc aggaagaaat cgaattggtt ggcgagaatg cggcgagcat
     1681 tgatgcttat gctgccagtg atcctgctga atgttttgcc gtactttctg aatatttctt
     1741 tagcgcccca gaactttttg ctcctcgttt cccttcattg tggcaacgtt tctgccaatt
     1801 ttatcaacaa gatcctttgc agagactgca tcgcactaat gatacagatt cgttttcggc
     1861 gacgaatgtt cattaattaa caactttgca gattaattaa ccaattgaaa tgacttatga
     1921 aatttagtgt tgacagacaa ggtaccgcta agtaatatgc gccccgttca cacgattcct
     1981 ctgtagttca gtcggtagaa cggcggactg ttaatccgta tgtcactggt tcgagtccag
     2041 tcagaggagc caaattctaa aaattcgctt ttttagcgca atgtcactga ccttagttga
     2101 acattgtttt ttaacggata gcgggttttt aacatcttaa gcgccctcga cctttatggt
     2161 tgagggcgtt ttgctatgaa cgccatcacc attttcccct cgattataaa acttgagtta
     2221 ttcagtagtc tcccctcttg caactcacac tcaaaactgc ctaacgaaaa gttattaatt
     2281 ttcaatcata ttgctatcag gatttacatt ttttcgctgt gctagaaagg gcgcatttat
     2341 gttagctcgt tcagggaagg taagcatggc tacgaagaag agaagtggag aagaaataaa
     2401 tgaccgacaa atattatgcg ggatgggaat taaactacgc cgcttaactg cgggtatctg
     2461 cctgataact caacttgcgt tccctatggc tgcggcagca caaggtgtgg taaacgccgc
     2521 aacccaacaa ccagttcctg cacaattcgc cattgcaaat gccaatacgg taccctatac
     2581 ccttggagcg ctggaatcgg cccaaagcgt tgccgaacgc ttcggtattt cggtggctga
     2641 gttacgcaaa ctcaaccagt ttcgtacgtt tgctcgaggt tttgataatg tccgccaggg
     2701 tgatgaactg gatgtcccgg cacaagttag tgaaaataat ttaaccccgc caccgggtaa
     2761 tagcagtggc aaccttgagc aacagatagc cagtacttca cagcaaatcg ggtctctgct
     2821 cgccgaagat atgaacagcg agcaagcggc aaatatggcg cgtggatggg cctcttctca
     2881 ggcttcaggc gcaatgacag actggttaag ccgcttcggt accgcaagaa tcacgctggg
     2941 cgtggatgaa gattttagcc tgaagaactc ccagttcgat tttctccatc cgtggtatga
     3001 aacgcctgat aatctctttt tcagtcagca tactctccat cgtactgacg agcgtacgca
     3061 gattaacaac ggcttaggtt ggcgtcattt cactcccaca tggatgtcgg gcatcaactt
     3121 ctttttcgac cacgatctta gccgttacca ctcccgcgcc ggcattggcg cggagtactg
     3181 gcgcgactat ctaaaattaa gcagtaacgg ctatttgcga ctgaccaact ggcgcagcgc
     3241 acctgagctg gacaacgatt atgaagcccg cccggccaat ggctgggatg tacgcgcaga
     3301 aggctggcta cccgcctggc cgcaccttgg cggtaaactg gtctatgaac agtattatgg
     3361 cgatgaagtg gccctgttcg ataaagacga tcggcaaagt aatcctcatg ccataaccgc
     3421 tggacttaac tataccccct tcccgctgat gaccttcagc gcggagcaac gccagggtaa
     3481 acagggcgaa aatgacaccc gttttgccgt cgattttacc tggcaacctg gcagcgcaat
     3541 gcagaaacag cttgacccga acgaagtcgc tgcacggcgt agccttgcag gcagccgtta
     3601 tgatctggtg gatcgcaaca acaacatcgt tctggaatac cgcaaaaaag aactggttcg
     3661 cctgaccctg acagaccccg tgacagggaa gtcaggagaa gtgaaatcat tggtttcgtc
     3721 gctacaaacc aaatatgccc tgaaaggcta taacgtcgaa gccaccgctc tggaagctgc
     3781 cggtggcaaa gtggtcacaa cgggtaaaga tattctggtt accctgccgg cttaccggtt
     3841 caccagtacg ccagaaaccg ataacacctg gccgattgaa gtcaccgccg aagatgtcaa
     3901 aggcaatttg tcgaatcgtg aacagagcat ggtggtcgtt caggcaccta cgctaagcca
     3961 gaaagattcc tcagtatcgt taagtaccca aacattgaac gcggattccc attcaaccgc
     4021 cacactgact tttattgcgc atgatgcagc aggtaatcct gttgtcgggc tggtgctctc
     4081 gacgcgtcac gaaggtgttc aggacatcac cctttctgaa tggaaagata atggtgacgg
     4141 aagctatacc cagatcctga ccacaggagc gatgtctggc acgctgacgc tgatgccaca
     4201 gctgaatggt gtggatgcgg ctaaagcccc cgccgtggtg aatatcattt ctatttcgtc
     4261 atcccgaact cactcgtcaa ttaaaattga taaagaccgt tatctctccg gcaatcctat
     4321 cgaggtgacg gtagaactga gagatgaaaa tgacaaacct gttaaggagc aaaaacagca
     4381 actgaataac gcagtcagca tcgacaacgt gaaacctggt gtcactacag actggaaaga
     4441 aaccgcagat ggcgtctata aggcaaccta taccgcctat accaaaggca gtgggcttac
     4501 tgcgaagcta ttaatgcaaa actggaatga agatttgcat accgctggtt ttatcatcga
     4561 cgccaacccg cagtcagcaa aaattgcgac attatctgcc agcaataatg gtgtgctcgc
     4621 caatgagaat gcagcaaaca ccgtctcggt caatgtcgct gatgaaggaa gcaacccaat
     4681 caatgatcat accgtcacgt ttgcggtatt aagcggatcg gcaacttcct tcaacaatca
     4741 aaacaccgca aaaacggatg ttaatggtct ggcgactttt gatctgaaaa gtagtaagca
     4801 ggaagacaac acggttgaag tcacccttga aaatggcgtg aaacaaacgt taatcgtcag
     4861 ttttgtcggc gactcgagta ccgcgcaggt tgagctgcag aagtcgaaaa atgaagtggt
     4921 cgctgacggc aatgatagcg ccacaatgac cgcgacagtc cgggatgcaa aaggcaacct
     4981 gctcaatgac gtcaaggtca ctttcaatgt taattcagca gaggcgaaac tgagccaaac
     5041 agaagtgaat agccacgacg ggatcgccac agctacgctg accagtttga aaaatggtga
     5101 ttatagggtt
//
LOCUS       DAZKIV010000225         5103 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZKIV010000225 DAZKIV010000000
VERSION     DAZKIV010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.227"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..155)
                     /locus_tag="ACHOT6_004482"
     CDS             complement(<1..155)
                     /locus_tag="ACHOT6_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HFP9949573.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILGKRTA"
     gene            complement(245..1060)
                     /gene="allR"
                     /locus_tag="ACHOT6_004483"
     CDS             complement(245..1060)
                     /gene="allR"
                     /locus_tag="ACHOT6_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFP9949574.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCL
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(1138..1620)
                     /gene="allA"
                     /locus_tag="ACHOT6_004484"
     CDS             complement(1138..1620)
                     /gene="allA"
                     /locus_tag="ACHOT6_004484"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFP9949575.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVAVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            1850..2776
                     /gene="allS"
                     /locus_tag="ACHOT6_004485"
     CDS             1850..2776
                     /gene="allS"
                     /locus_tag="ACHOT6_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFP9949576.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            2845..3939
                     /gene="mnmH"
                     /locus_tag="ACHOT6_004486"
     CDS             2845..3939
                     /gene="mnmH"
                     /locus_tag="ACHOT6_004486"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFP9949577.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            4055..4420
                     /gene="ylbG"
                     /locus_tag="ACHOT6_004487"
                     /pseudo
     CDS             4055..4420
                     /gene="ylbG"
                     /locus_tag="ACHOT6_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         1301 a         1281 c         1249 g         1272 t
ORIGIN      
        1 gctgtacgct tacccagaat gtgacgaata ccgccgtgct tacgcatcgc tgagtagaac
       61 ggatttattg cagctcccgg aacgccgaag gcggtggtga taccttcttt ctccagcaca
      121 tacattgccg cgtcaacggc tctcattttt gccattttat tcctacctct attaattgga
      181 aaatttttcc aactttcaaa ttttgtcaaa aaaacccgct ccgcagagcg ggatgcgaca
      241 gacattatgg atgtgctttc agtcccaacg ccgtgctgat atcgcgggcg gtgtctctga
      301 ccagctcacc ctgactgaca aaacgatctt ctgtcagtct tgatgaaggc ccggagatgg
      361 agatagcggc aacaacacta cctacatcat cgtaaattgc tgaagctaag caattcagac
      421 ctacaacatg ctcttcttta tctacggtat agcccagttc acgcgcttgt tccaggtcct
      481 tcagcaaggt gggcatatcc acaagcgtag ttggcgtaaa ctgctgcaaa ccggtttgca
      541 gaatgatgct catcaactcc tcttccgcca gcggataaag cagcgctttg cccgcaccgg
      601 aagcatgcag cggcagacga ctgcccagtg gcgcacacat cctgaccatt gatttacact
      661 ctaactgacc aattaatact gcttcattgc cgttacggat cgcgacattg accgtttcgc
      721 cggaaagtaa catcaggcgg cgcataaacg gcccggcgac ggagaggaca tcgcggttat
      781 ggatgtacgc cgcaccgacg ttaaagacac ctaatcctat atgccaccag cctaattggc
      841 tgtcctgata gacaaaatcc gctgcttgta aaaccttcag caagcgaaag gtcgtggaga
      901 gcggcaaatc cagattgaga gaaatatcgc taaccgacga gcttccccca cttttttcca
      961 gatattgcag aatcgcaatt ccccgctcta acgcctgtgc gcccttctgt gccacaggct
     1021 ccgcctgtcc tggcctgccg cgccgtctaa cttccgtcat acctacgtta actccgacct
     1081 acaagtgagc ataaacaatg aacaattagt ttatcttatg caagtcggtt gcaggcgtca
     1141 cgcaaaacag agttcctgtt caggaatact ttcaacatca cagttgtcac tgccgccgcg
     1201 atcgatggtc agaaaatcgg tgacgcgctg ccaggcgaaa agtgggtgat gccagacgtt
     1261 acgatggtaa ttcactccct gttcgccgtt ggtgataaac gcccgcagcg ttgacaggtc
     1321 tggtttgtcg tcgcctaacg ccacggccac cacaaacact tcacctttca tcgggataaa
     1381 ggcctgagtg cccagcggat gacgttcgag ttcgtgaatg gtcagcggca gattcgccgg
     1441 ttgcgcgcgg ttaatgctga taagcgtacg gtcttgctca agaatctcaa ccagcgccaa
     1501 atcgtggtaa cgctccacca ggccattgtt aatatggaaa aaatcccgtt gctgcgtttc
     1561 gattacgtcg ccataagcac taaaggcttc ctgacttaac ggtaatacct gaagtttcat
     1621 ctttttgtcc ttgtaacata attccgttca accaccgccg accggtcagc ttggtttcaa
     1681 taacaggcaa tctaaaacct taagagaaca acaccaatac aaaagttgga atatttttcc
     1741 aaaactagat ttagtccaca aattgaacag ggttatcatg taaaaatact gataaatcag
     1801 aggataagaa aatcacgcaa cttgcggatt taaaagggga tacctgaaga tgttcgatcc
     1861 agaaaccttg cggactttca ttgcggttgc tgaaacagga agtttttcaa aagcggcaga
     1921 acgattatgt aaaaccacgg cgacgatcag ttatcgcatt aaacttctgg aagagaatac
     1981 cggagtagcg ctgtttttcc gtacgactcg tagcgtgacg ttgacagcgg ctggcgagca
     2041 tctactttcc caggccagag actggctgag ctggctggaa agtatgccaa gcgagctgca
     2101 acaggtgaat gatggcgtgg aacgccaggt gaatattgtc atcaacaacc tgctctacaa
     2161 cccccaggcc gtcgcccagt tgctggcgtg gctgaatgaa cgttacccct ttacccagtt
     2221 tcacatctcc cgacaaatct atatgggcgt ctgggattcg ctattgtacg aaggtttttc
     2281 actggctatc ggcgtcacgg gaaccgaggc gctggcaaat acctttagtc ttgatccctt
     2341 aggatcggtg caatggcgct ttgtcatggc ggcggatcat ccgctggcga acgttgaaga
     2401 gccgctaaca gaagcgcagt tgcggcgctt tccggcggtc aatattgaag acagcgcccg
     2461 caccttaacc aaacgcgtcg cctggcgatt gccagggcaa aaagagatta ttgttcctga
     2521 tatggaaacg aaaatcgccg cccatcttgc gggcgttggc attggttttt tgccaaaatc
     2581 gctttgccag tcaatgatcg ataatcaaca actggttagc cgggtaatcc caacgatgcg
     2641 ccctccttcg ccattgagtc tggcatggcg caaatttggc agcggcaaag cggtagaaga
     2701 tattgtgacc ttgtttaccc agcgcaggcc ggaaatcagc ggatttttag aaattttcgg
     2761 caacccacgc agttaaaaat caagtttcct tgcgcgttgg cgcgtaaaat agcgcgcaag
     2821 ctgatttgag gttcactttt aactatgcaa gagagacaca cggaacagga ctatcgtgcc
     2881 ctgctgattg ctgatacgcc cattattgat gttcgcgccc ctatcgagtt tgagcaaggc
     2941 gcaatgcccg ccgctatcaa tctgccgtta atgaataacg atgaacgcgc cgccgttggc
     3001 acctgctata aacagcaagg ctcagacgca gcgctggcgc tggggcataa actggtggcg
     3061 ggtgaaattc gtcagcagcg catggacgcc tggcgggcag cgtgcctgca aaatccgcaa
     3121 ggtattctct gctgcgcccg tggcggtcag cgctcacata ttgtgcaaag ctggttgtat
     3181 gcagcgggga ttgattatcc gctggtggaa ggcggttata aggcactgcg ccagaccgcg
     3241 attcaggcga ctattgaact ggcacaaaaa ccgatagtgc tgattggcgg ttgtactggc
     3301 agcggtaaaa cgctgttagt gcagcaacag ccgaacggtg ttgatctgga agggctggcg
     3361 cgccatcgcg gttcggcgtt tggtcgcacg ttacaaccac aacttagcca ggcgagtttt
     3421 gaaaacctgc tggctgccga aatgctgaaa accgacgccc gtcaggattt gcgcctgtgg
     3481 gtgctggaag acgaaagccg gatgatcggt tcgaatcacc tgccggaatg tctgcgcgag
     3541 cgaatgactc aggcggcgat tgcggtggta gaagatccgt ttgagatccg tcttgagcgc
     3601 ctgaacgaag agtatttctt gcgtatgcat catgatttta cccacgctta tggcgacgaa
     3661 cagggctggc aggaatattg cgaatacctg catcacggac tttcggcgat taagcgtcgg
     3721 ctggggttac agcgctataa cgaactcgct gcccagctgg atacggcgct gacaacgcaa
     3781 ctcaccaccg gcagcaccga cggtcatctg gcctggctgg tgccgttact taaagaatat
     3841 tacgacccaa tgtatcgcta tcagcttgag aaaaaagcgg aaaaagttgt ctttcgcggt
     3901 gagtgggcgg aagtggcgga atgggttaag gcgcggtaat gagggacgta tttgtattag
     3961 tcgcgacttg atctgacaca tggccttgaa aggttgagag ttaccggttt tgatatgagt
     4021 gtcgaatcct tatccaaaac aagaggtaac tctcatgctt catactgcca atcccgtcat
     4081 caaacacaaa gccggtttgc tcaatctggc tgaagaactc agcaacgtat caaaagcctg
     4141 taaaatcatg ggcgtttcgc gtgatacgtt ttatcgttac cgcgaactgg tcgctgaagg
     4201 cggtgtggat gcgcagatta atcgtagtcg ccgcgctcct aaccttaaga accgtaccga
     4261 tgaggcaact gaacaggctg ttgttgatta cgccgtcgct ttcccgacac atggtcagca
     4321 ccgggccagc aacgagctgc gtaaacaggg ggtttttatc tccgacagtg gtgtccgttc
     4381 tgtctggtta ctccataacc ttgagaatct caaaagacga tactgaacaa attttatcag
     4441 gtgacgttcc gtaaaaagtt gtatggagtt ctcgatgcag tcggcgcgac tatcagtacc
     4501 cccgctgaac cctggactat cgggccgccg tactggggca tgtcaccctg gcgtgccgct
     4561 ggttttccgc taatggttta tttcctcttt ctttcccggc aagaatttat tttaatccgg
     4621 aaggattatt tattttcaga tagggactca cattaaacac gtaacatttt aattaatgca
     4681 tgactgaata tgtattacac aatgtcagat atcgtaaaag accagtgcaa ttctatgtaa
     4741 actctctgac tgttcatttt atttgttgtt tcagggtcgg aactcaccct caataaacaa
     4801 ctgaatacga gtagatattc atcgtctgag ctatatggct ttacacaata gccgacatgt
     4861 cttttctcag tcatgaattt tacttttcct cactacgaag ttgacgactt cgatatggga
     4921 tagactctta attcaagcaa ttactctgca caagcatcag gcaatccagc tccaatattt
     4981 gttataaaat aatgcgtaac attaatttca ccaacaaaaa aaggtttacg ccatctcctt
     5041 gccctggtat cttaccatat ttacaccaca ctttttttgt atctggatca ttctttgata
     5101 cga
//
LOCUS       DAZKIV010000226         5008 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZKIV010000226 DAZKIV010000000
VERSION     DAZKIV010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.228"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(118..876)
                     /gene="yhdZ"
                     /locus_tag="ACHOT6_004488"
     CDS             complement(118..876)
                     /gene="yhdZ"
                     /locus_tag="ACHOT6_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFP9949578.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(884..1987)
                     /gene="yhdY"
                     /locus_tag="ACHOT6_004489"
     CDS             complement(884..1987)
                     /gene="yhdY"
                     /locus_tag="ACHOT6_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9949579.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1997..3178)
                     /gene="yhdX"
                     /locus_tag="ACHOT6_004490"
     CDS             complement(1997..3178)
                     /gene="yhdX"
                     /locus_tag="ACHOT6_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFP9949580.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3246..4271)
                     /locus_tag="ACHOT6_004491"
     CDS             complement(3246..4271)
                     /locus_tag="ACHOT6_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9949581.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4702..4923)
                     /locus_tag="ACHOT6_004492"
     CDS             complement(4702..4923)
                     /locus_tag="ACHOT6_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949582.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
BASE COUNT         1384 a         1297 c         1183 g         1144 t
ORIGIN      
        1 gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc cagacatcaa
       61 atcaagcgaa aggccatccg aaaggatggc ctttttgctt ttcgaactaa cattcaatta
      121 atggattacc tgcgataaaa atgccctcgt acgctctgat ttaggatgcg caaaaaattc
      181 atcaggtgca gcttgttcca ctatctcccc acgatccata aaaattaccc ggtcagcgac
      241 ggtacgtgca aaccccatct catgtgttac acacaacatt gtcatacccg actgcgccag
      301 cccaatcatc gtatccagca cctctttcac catctccgga tcgagcgccg acgttggttc
      361 atcaaacaac ataattttcg gcttcataca cagcgaacgc gcaatggcaa cgcgttgctg
      421 ctgaccacct gaaatctgtc cgggaaactt atgcgcatgt tcggcaattc tcacccgctc
      481 aaggtaatgc atcgccagag cttcagcctc tttcttaggc atcttgcgta cccaaatcgg
      541 tgccagggta cagttctgta gaacggtcag atgagggaag agattgaaat gctgaaagac
      601 cattcccact tcctgcctga cgcgctcaat attgcggata tcttcattaa gttcgatgcc
      661 atctactacg atccgtccct gttgatgttc ttccagatga ttaatacaac gaatggttgt
      721 tgatttaccg gaaccagaag ggccacacag aacgatccgt tctcccggtt gcacggttaa
      781 atttatattt ttcaaaacat ggaattgtcc ataccattta ttgacgtttt ccagcgtaat
      841 catcgcgtta gcaggttgca gtaaaatttg gctcatagtg tcctcaatgc ggtgtacgcc
      901 cggtgttaaa acgtttttcc agatgctggc tatagcgcga catgctgaaa cagaagatcc
      961 agtagatcag cgcggcgaaa acataccctt ccgtcgacat acccaaccag gcgggatcaa
     1021 cggttgcctg ctgaacgcta ctgaaaagat cgaacaaccc gataatgatc accaggctgg
     1081 tatctttgaa gagtgcgatg atggtattta ccagcccagg aattaccagc ttcaacgcct
     1141 gtggcagaat aaccagcccc tgagttttcc agtaacccaa tgccagtgac tctgccgctt
     1201 catattgccc tttaggcagc gcctgtaatc cgcctcgcac gacttccgca acatatgctg
     1261 actgaaacag gatcacgcca accagcgcgc ggatcagttt gtcgatattg gtgccttctg
     1321 ccataaacaa aggcagcatg accgaagaca taaacaatac ggtaatcagc ggtacgccgc
     1381 gccagaactc gataaaaatg accgataaga tacgcacaat cggcatatgg gagcggcgac
     1441 ctaacgccag taagatcccc cacggtagcg cccccgcaat cccaacggat gcaataatta
     1501 aagttagcgt cagcccgccc cattgccggg tttcaacccg ctccagacca aaaaaaccgc
     1561 catacatcag ccaccagaca atcagtgggt aaatcaccgc ccagaccgca atatagcgac
     1621 cgcgatgcgg gagtattttc cagaacattg gtgcgattga cacaaggcca atcagtaatg
     1681 ccaggttaat tcgccagcgt tggtcgtgtg ggtaaagtcc atacataaac tgaccaaatc
     1741 gttcgtggat gaagacccaa caggcaccgg cttttgtgca gtcggcacgc gttgagccaa
     1801 cccaattagc ctgcaaaaat gcccagttca gcaacggtgg gatcagttcc cacatcaacc
     1861 atatgcagcc aatagtcagc aggctattgc tccagctgga gaacagattt tttcgcaccc
     1921 acaccatcgc gcggcttgag ttatggctcg ctgggcgcga gggctgagac agcaatactt
     1981 ttgtcatcat gactccttag cgttcaacga tcgcgatgcg gcggttatag atattcatca
     2041 gcagcgagat agtcaggctg ataatcagat agaccgacat ggtcatggct atcgtctcga
     2101 tggcttgccc cgtctggttc agcacggtgc cagcaaacag cgaaaccata tcgggatagc
     2161 caatagcggc ggcaagagag gagtttttga cgatgttgag atactggctg gttaacggcg
     2221 gaataatcac tcgcagtgcc tggggaataa tgacctggcg tagcgtaacc gggttgggta
     2281 atcccagtga ccgagccgct tcatgttgac cataaggcac tgcctggatc ccagcgcgga
     2341 taatctcggc gataaatgca gatgtataaa ccgaaagtgc cagcgttaag gctgccagtt
     2401 caggaattaa aaccatcccg ccgcggaagt taaagcctcg tagggctggg acatcccagt
     2461 gtagtgctgc gccaaaaagc cattgcgcca gtaaaggcaa accaatgatc aacactgcgg
     2521 cgatcggcca ggtgcggcgc agttgtccgg tctttatctg gtatgtttta ttaaagcgga
     2581 atagcccaac agaaaggact atagccataa caacagccag gataaacgca ataaatccgt
     2641 ctcccagctg cggtgacgga atataaagcc cacgattgct caaaaaggcc agatcaaacg
     2701 cgctcacagc ctggcgtggt ccgggcaaat tgcgcaacac ggcaaagtac cagaagaaga
     2761 tttgcagcag cgggggaata ttacggaaga tctcaatata aattgtggat agttttcgta
     2821 gcagccagtt atccgaaagt ctcgccagac cgataaagaa gcctaaaaca gaagcgaaca
     2881 caatacacaa tgcagaaacc agtagcgtat tgagtaagcc gacaataaaa acgcgtccgt
     2941 aggtgtcgcc ctgctggtaa tcgatcaaat gctggacaat accgaagcca gcgccgcgat
     3001 ccagaaaggc aaaacctgaa gtaatgccac gattattgag attcgttaca gtgttgtgaa
     3061 ataaccaacc aacaatgccg acaacagcaa caacggcaag gatctggaat aaccaggcgc
     3121 gaaccgtagg gttggcaaaa gagagtgagc ctttaacggt tgagcggcga tgagacatag
     3181 aaacctcgga aagcataacg ctgaagagag cgccgccgta gcgacgcccg ttacctcaca
     3241 ccgacttaac gcacgggcgg cgcgtactga ataccgccgt tattccagag attattttgc
     3301 ccacgtttaa ttttcagcgg gctttctgaa cctacgttac gctcgaaaat ttccgagtag
     3361 ttacccacct gtttgatgat gttataggcc catttattat ccagcttcag atccttgccg
     3421 taatcgcctt ctttacccag cagatgtgcc atatcaggcg ttgctggatt agccgctttt
     3481 tcatcgacgt tctgggaatt gatgcccatc tcttcagcat tcagcatggc gaaaagcgtc
     3541 cagcgtacaa tcgagaacca ttcatcatcg ccacgacgaa ctaccggccc aagcggttct
     3601 ttagagataa cctccggtaa gacaatccat tcagctgggt tgcttaattt gatgcgcagg
     3661 gcatacagtt gcgactgatc cgaggccagc gtatcgcagc gaccagattc cagtgccttc
     3721 gctgattcgt cagagcgatc gaaagtcacc ggtgtgtact tcatattgtt tgccttgaaa
     3781 tagtcggcaa cgttgagttc ggtatcagta cccgcctgaa tacagacggt agcgccatcc
     3841 agttctttcg cgctttttag ccccgcttta tcgtgcgtca ggaagccaat gccgtcgtaa
     3901 taagtgacgc cggtaaatgc cattcccatc ccggcatcgc gagatgaagt ccaggtcgta
     3961 ttacgggaga gcaaatccac ttcccctgac tgtaaagcgg tgaagcgttc ttttgctgtg
     4021 agtggggtat atttcacttt cgtgtcgtca ccaaatacag cagcggcaac accacgacaa
     4081 acatcaacat caatacctga aaacttaccg tctgcatcgg cataagagaa cccaggtaat
     4141 ccatcactga tcccgcattg cacaaaacct tttttctgaa cggcatcaag cgtcgcgcca
     4201 gcatgcgcct gatttgcaac ggcaagcagc acgctggcgg cagccagtgt ggctatcatc
     4261 atctttttca taatgcttcc tgtgaggcga aaattatcgt tataggacgt ttgggaaacg
     4321 tcttttacct gtctgtggcc atcctcttat aagcaaaagt agtgccagct tttgcgcacg
     4381 atttcgcgtc gcaataagct gagtgtagac aggaaataat aaaaacagcg ccccgcaatg
     4441 gtgcgaaaat gaaacatgcg ccacattttg gcgcaaaata tttacgcgtg ttttactaag
     4501 cagaataatg attgttagcg tgagtatatt tctggtggga agtgtgaaag ttaaattgat
     4561 gcgttatttc gaaagcagcg aaataacgcg atataacatg ctaaatcagc agagaattag
     4621 ccagcagaat atggcaatat tttatcttat gtaaagatac tgtatcgtta aacagagcgc
     4681 gaaactccgc gccctgttca gttatttcga cagagcgatg atccccatcg tcaggccagc
     4741 aacagccgct gccccgccgg caatagcccc agccgtttcc cagttatcct gagtcgtgcc
     4801 tttcatacag gtattggtgt gaaccagacg accctgatcg tcataaaccg gaacgcaggg
     4861 cgaatcgtga gcacagcccg ccagcaatgc ggtgagcaat gccacaggaa ttaatctttt
     4921 catggtgtta cctcagtaat aaacgctaac gcttaataag ttaactctct tattaacgag
     4981 ctgaatgcca ttttatcacc ggaggtta
//
LOCUS       DAZKIV010000227         5002 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZKIV010000227 DAZKIV010000000
VERSION     DAZKIV010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.229"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..1257
                     /gene="frc"
                     /locus_tag="ACHOT6_004493"
     CDS             7..1257
                     /gene="frc"
                     /locus_tag="ACHOT6_004493"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFP9949583.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            1311..3005
                     /gene="oxc"
                     /locus_tag="ACHOT6_004494"
     CDS             1311..3005
                     /gene="oxc"
                     /locus_tag="ACHOT6_004494"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFP9949584.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVIL
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            3075..4019
                     /gene="yfdV"
                     /locus_tag="ACHOT6_004495"
     CDS             3075..4019
                     /gene="yfdV"
                     /locus_tag="ACHOT6_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFP9949585.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            4093..>5002
                     /gene="yfdE"
                     /locus_tag="ACHOT6_004496"
     CDS             4093..>5002
                     /gene="yfdE"
                     /locus_tag="ACHOT6_004496"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9949586.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAA"
BASE COUNT         1308 a         1064 c         1214 g         1416 t
ORIGIN      
        1 attgctatgt caactccact tcaaggaatt aaagttctcg atttcaccgg tgtgcaatct
       61 ggcccatctt gtactcaaat gctggcctgg tttggcgctg acgttattaa aattgaacgt
      121 cccggcgttg gtgacgtaac gcgtcaccag ctgcgagata ttcctgatat cgatgcgctt
      181 tacttcacca tgcttaacag taacaaacgt tctattgagt taaataccaa aacagcggaa
      241 ggcaaagagg taatggaaaa gctgatccgc gaagctgata tcttagtcga gaactttcat
      301 ccaggggcca ttgatcacat gggcttcacc tgggagcata ttcaagaaat caatccacgt
      361 ctgatttttg gttcgatcaa agggtttgat gagtgttcgc cttatgtgaa tgtaaaagcc
      421 tatgaaaacg ttgctcaggc agcgggtggc gcggcatcca ctacgggttt ttgggatggt
      481 ccgccgctgg taagcgctgc agcgttgggt gacagcaaca ccggaatgca tttgctgatc
      541 ggtttacttg ctgctttgct gcatcgcgaa aaaacggggc gtgggcaacg agtcaccatg
      601 tcaatgcagg atgccgtatt gaacctttgc cgcgtgaaat tacgtgacca gcagcgtctc
      661 gataaattgg gttatctgga agaatacccg cagtatccga atggtacatt tggtgatgca
      721 gttccccgcg gtggtaatgc aggtggtggc ggtcagcctg gctggatcct gaaatgtaaa
      781 ggctgggaaa ccgatcctaa cgcctatatt tatttcacta ttcaggagca aaactgggaa
      841 aacacctgta aagccatcgg caaaccagaa tggattaccg atccggcata cagtacagcc
      901 catgcacgac agccacatat tttcgatatt tttgctgaaa tcgaaaaata cactgtcact
      961 attgataaac atgaagcggt ggcctatttg actcagtttg atattccttg tgcaccggtt
     1021 ttaagtatga aagaaatttc acttgatccc tctttgcgcc aaagtggcag tgttgttgaa
     1081 gtggaacaac cgttgcgtgg aaaatatctg accgttggtt gtccaatgaa attctctgcc
     1141 tttacgccgg atattaaagc tgcgccgcta ttaggtgaac ataccgctgc tgtattgcag
     1201 gagctgggtt atagcgacga tgaaattgct gcaatgaagc aaaaccacgc catctgatat
     1261 taacgggggc ttctggctcc caatttataa aaatttcgag gttattaatc atgtcagatc
     1321 agcttcaaat gacagatggt atgcatatca tcgttgaagc attaaaacag aataatattg
     1381 acactattta tggtgttgta ggtattcctg tgacggatat ggcacgccat gcccaggcgg
     1441 aaggcattcg ttatattggt tttcgtcatg agcagtcggc aggctatgcc gctgcggcaa
     1501 gcggttttct tacccaaaaa ccggggatct gcctgacagt ttctgcgcca ggattcctca
     1561 atggtttgac cgcattggcc aacgcaacgg taaatggttt tccgatgatc atgattagcg
     1621 gctccagcga ccgcgcgatc gtcgacctac agcaaggtga ttatgaagag ctggaccaaa
     1681 tgaatgcggc aaaaccgtat gccaaagcag catttcgcgt taatcagccg caggatcttg
     1741 gcattgcatt ggcacgcgct atccgggtct ctgtatcggg tcgccctggc ggagtttatc
     1801 ttgatttgcc agcaaatgtc ctggccgcga cgatggaaaa agacgaagcg ttaactacga
     1861 ttgttaaagt tgaaaatccg tcgccagcat tattgccatg cccgaagtca gtcactagcg
     1921 caatttcgct tttagcaaaa gctgaacggc cattaattat ccttggcaaa ggcgcggcgt
     1981 attcacaagc tgatgaacag cttcgtgaat ttattgaaag tgctcagatt ccattcctgc
     2041 caatgtctat ggcgaaaggg atccttgaag atacgcatcc actttctgcg gcagctgcgc
     2101 gttcgtttgc cctggcaaat gctgacgttg tcattcttgt tggtgcacga ctgaattggt
     2161 tattggcaca cggtaaaaaa ggatgggcgg cagatacaca gtttattcaa ctggatattg
     2221 aaccgcagga aattgacagc aaccgcccca ttgctgtgcc agtcgttggt gatattgcat
     2281 ccagtatgca aggtatgctg gcagaactga aacaaaacac atttacgact ccactggtat
     2341 ggcgcgatat tttaaatatc cacaagcagc aaaatgcaca aaaaatgcat gaaaaattaa
     2401 gtacagatac tcaaccatta aattacttta atgcattaag tgctgtgcgc gacgtattgc
     2461 gcgagaacca ggatatttat ttagttaatg aaggtgcaaa taccctggat aatgcacgaa
     2521 atattattga tatgtataaa ccacgtcgtc gtctggattg tggtacctgg ggtgtcatgg
     2581 gcatcggtat gggctatgcc atcggtgcta gcgtgacttc tggttctccg gttgtcgcca
     2641 ttgaaggtga tagtgctttt ggtttcagtg ggatggaaat tgaaacgatt tgtcgatata
     2701 acctgccggt gacgatcgtt atttttaata atggcggcat ctacagagga gacggtgttg
     2761 atctcagtgg cgctggtgca ccatcaccaa cggatctgtt gcaccatgca aggtatgaca
     2821 aattaatgga tgcgtttcgt ggcgttggct ataacgtcac cacgacagat gaacttcgtc
     2881 atgctttaac caccggtatt cagtcgcgca aaccgaccat tattaatgtg gtcatcgacc
     2941 ctgcagcagg aactgaaagt ggccatatta ccaaacttaa cccaaaacaa gtcgctggta
     3001 attaaatact gacatcgtct cctcttgcca actcattatg cgatggtgag gggaccctta
     3061 tcggaaatac tattatgcta acatttttta ttggcgattt attgcctatt atcgtaatca
     3121 tgctgttggg ttattttagc ggcagacgag aaacattttc agaagatcaa gctcgggcat
     3181 ttaataaact ggtattaaac tacgcgcttc ctgcggctct atttgtatct attactcggg
     3241 caaacaggga aatgattttt gcggacactc gtctgaccct tgtatcactt gtggttattg
     3301 tcggatgttt ctttttctcc tggttcggtt gctacaaatt ttttaaacgt acccatgcag
     3361 aagcagctgt atgtgcatta attgcaggtt cacctaccat tggattcctg gggtttgcag
     3421 ttctcgatcc tatttatggt gattccgtat caacaggttt agtggtagca attatttcta
     3481 ttattgttaa cgcaattact attcctattg gtctgtattt gctgaatcct tcttcaggag
     3541 cggatggtaa gaagaatagt aatctgagtg cattaatttc tgcggcaaag gagccagtag
     3601 tatgggcacc tgttctggca acgatcctgg tgttggttgg ggtaaaaatt ccggcagcat
     3661 gggacccaac ctttaatctg attgcgaagg ctaactcagg ggtagcggta ttcgctgcgg
     3721 ggttgactct ggctgcacat aaattcgagt tcagtgccga aattgcttat aacaccttcc
     3781 tgaagctgat tctgatgcca ctggcactgc ttctcgttgg tatggcatgt catttgaaca
     3841 gcgaacatct gcagatgatg gtactggcag gcgcattacc gccggcattc tccggaatca
     3901 ttattgccag ccggtttaat gtctacaccc gtactggtac agcgtcattg gctgtgagcg
     3961 tactgggctt tgtcgtcacg gctcccttgt ggatttatgt cagtcgactg gtttcataat
     4021 taatcatgtt attgatttta cataaatgcc gaagtgtctt ttcaccttcg gcttttttca
     4081 aggcataagc aaatgacaaa taatgaaagc aaagggccgt ttgaaggctt attagttatc
     4141 gatatgacac atgtccttaa tggacctttc ggaactcaac ttctttgtaa tatgggcgca
     4201 agggtaatta aagttgagcc gccgggtcat ggtgatgata cccgcacatt tggtccctat
     4261 gtggatggac agtcactcta ttacagtttt attaatcatg gcaaagagag tgtggttctt
     4321 gatttaaaga atgatcacga taaaagtata tttataaata tgctcaaaca agctgatgta
     4381 ttagctgaga attttcgccc aggtacaatg gaaaaactgg ggttttcatg ggaaacgctt
     4441 caagaaatca acccgcgcct catatatgct tcatcgtcag gtttcggaca taccggtccg
     4501 ctaaaagatg ctcctgccta cgataccatc attcaggcaa tgagcgggat aatgatggaa
     4561 acaggatatc ctgatgctcc gccagtgcgc gttggtacct ctcttgcgga tctatgcggc
     4621 ggtgtctatt tattcagcgg aatagtgagt gcactttatg gccgcgaaaa gagccagaga
     4681 ggggcgcatg tcgatatagc gatgtttgat gccacgctga gttttctgga gcatggtctg
     4741 atggcatata tcgcaactgg gaagtcacca caacgtctgg gaaatcgcca tccctacatg
     4801 gcaccttttg atgttttcaa tactcaggat aagccgatta cgatttgttg tggtaatgac
     4861 aagctttttt ctgcgttatg tcaggcactg gagcttacgg aactggttaa tgatccccga
     4921 tttagcagca atattttacg cgtacaaaac caggctattc ttaaacaata tattgagcgg
     4981 acgttaaaaa cgcaggcagc tg
//
LOCUS       DAZKIV010000228         4970 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZKIV010000228 DAZKIV010000000
VERSION     DAZKIV010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.230"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(51..524)
                     /gene="ysaA"
                     /locus_tag="ACHOT6_004497"
     CDS             complement(51..524)
                     /gene="ysaA"
                     /locus_tag="ACHOT6_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFP9949587.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(626..1474)
                     /gene="yiaJ"
                     /locus_tag="ACHOT6_004498"
     CDS             complement(626..1474)
                     /gene="yiaJ"
                     /locus_tag="ACHOT6_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFP9949588.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            1675..2673
                     /gene="yiaK"
                     /locus_tag="ACHOT6_004499"
     CDS             1675..2673
                     /gene="yiaK"
                     /locus_tag="ACHOT6_004499"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFP9949589.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            2685..3152
                     /gene="yiaL"
                     /locus_tag="ACHOT6_004500"
     CDS             2685..3152
                     /gene="yiaL"
                     /locus_tag="ACHOT6_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFP9949590.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            3280..3744
                     /gene="yiaM"
                     /locus_tag="ACHOT6_004501"
     CDS             3280..3744
                     /gene="yiaM"
                     /locus_tag="ACHOT6_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFP9949591.1"
                     /translation="MLEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYLFVW
                     LTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLKTIQ
                     DWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            3747..>4970
                     /gene="yiaN"
                     /locus_tag="ACHOT6_004502"
     CDS             3747..>4970
                     /gene="yiaN"
                     /locus_tag="ACHOT6_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFP9949592.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLV"
BASE COUNT         1162 a         1261 c         1220 g         1327 t
ORIGIN      
        1 ggcataaaca aagcgcacta ttccggcgtt gagaaacgcc ggaaaacgtt tcaaacaggc
       61 tgctgccgta gccgatgccg ctgcactttc tcgacatcca tgcactgcaa cgcctttgtc
      121 gggcaggctt caacacaggc cggccccgtc tcccgatgcc agcacagatc gcacttaatc
      181 gccctcgcct ttttgcgcga agaaacgacc tccatcgcac caaacgggca agccagcata
      241 cagcttttac agccaatgca acgtgtttgt tcaacgaaaa tatgcccatg ttcgcggctt
      301 atcgcgtcaa cagggcagac attcgcgcac ggtgcatctt cacactgatg acaggctact
      361 gccgtggtcc agctgtggtc tttaatgaca cgaatacggg aaataaactc gtctggtgac
      421 aacgcagcgc aatcctgatt ctcctgatgc gacactgcgc aagccacttc acaggtacgg
      481 caaccgatac atttcgtcgc atccgcaata ataaaccggt tcatgatcac tcctttgcac
      541 taaaacagca gagtgccaga gagcgcgtcc ctactgcttt gatccaacga aggaaatgtt
      601 aaagttttgg cacttgtcca aagcgttatg tgatcgcgcc ctggtcgtcc ctgacagtaa
      661 atcccagttc attagaaata gcctgcgcgg tttcacgcag tggtttcagg agatttttct
      721 cccccacctg tttcagacgt gatgtcgaaa gcgaaatcga cacggcgtac ggcacccgcc
      781 catgaatatt aaacaccgga acagcaatac aggagacgcc gagttcgttt tcttctctgt
      841 ccatcgccgc tccgctttca cgaatgtgcg ccagttcgtc gaacatcgcg ggcagctcgg
      901 taatggtatt gcgggttaac ggctggatct catgctgatg gttttcccag tatgacttca
      961 cgtagtccgg gtgaccaaac gccatgtaga tcttgcccat tgcggaacag tagagcggca
     1021 tatgctggcc aatataggca cgggttcgca gcatcccggt tgtgggttcc agcttataaa
     1081 tcaaaatagc gtgatcgtct tcgcggctgg agaagttaat ggtttcacca gtggcgatgt
     1141 tcagtgcctc aagatgcgga gcggcgatat gaatgatatt cagcgaagac agcgccttct
     1201 gcccgacggc aataaatttg gtagtcaggc ggtaactccc tgcggcgggc gcggtggtca
     1261 catagccgca ggactgtaat ccctgcaata agcgatggac ggtactctta tttaaaccag
     1321 ccagctccga aagatgcgcc aacggacaac cgtttggata gttgctcaaa atctcaatca
     1381 gcatcaaccc acgaaacaga ctctggcttc cggctggacg ctctttttcc tgcgccatct
     1441 cgttctcttt ttttcccatc acttctttcc ccattttgtc gcgtcctgat ggtagcgcaa
     1501 agtgtgccgt agttcacgat ctcgacagat aatttataac cacttgattt ttatgtcttt
     1561 tgaaattcat caatcagatt gctttgttaa aaagtgatcg atatatttga aatcaagttt
     1621 cgcatattga aattttaagc caaaaaagcg atcaaaaaaa caaggaagcc tgggatgaaa
     1681 gtgacatttg agcagttaaa agcagccttt aatcgggtct taatttcacg cggcgttgac
     1741 agcgaaacgg ctgacgcctg tgcagagatg ttcgcccgca ccaccgaatc cggcgtttat
     1801 tctcacggcg ttaatcgttt ccctcgtttc attcaacaac tggaaaacgg cgatatcatt
     1861 cctgatgccc aacccaaacg tataaccagc ctcggcgcaa ttgaacagtg ggatgcccag
     1921 cgttcgatcg gcaacctgac ggcgaaaaag atgatggatc gcgccattga actggctgcc
     1981 gatcacggta ttggtctggt ggcacttcgt aatgccaacc actggatgcg cggcggcagc
     2041 tacggctggc aggcggcgga aaaaggctat attggcattt gctggaccaa ctccatcgcc
     2101 gtaatgccgc cgtggggcgc aaaagagtgt cgcattggca ctaacccgct gatcgtcgcc
     2161 attccttcca cgccgatcac catggtcgat atgtcgatgt cgatgttctc ttacggcatg
     2221 ttagaagtta accgcctggc gggccgtcag ctcccggtcg atggtggctt tgatgatgag
     2281 ggcaatttga ccaaagaacc tggcgttatc gagaagaatc gccgcatttt gccgatgggc
     2341 tactggaaag gttctggcat gtcgattgtg ctggatatga tcgctactct cctttccgac
     2401 ggcgcatcgg ttgccgaagt cacccaggac aacagcgacg aatacggcat ttcacaaatt
     2461 tttattgcca ttgaagtgga caagcttatc gacggtccca cccgcgatgc caagctgcaa
     2521 cgtatcatgg attacgttac taccgctgaa cgcgctgacg aaaaccaggc catccgctta
     2581 cccggccacg aatttactac cctgctggcc gaaaaccgcc gtaacggtat taccgttgat
     2641 gacagcgtgt gggccaaaat ccaggcgtta taaggagata agtcatgatt tttggacata
     2701 tcgcgcagcc caatccgtgc cgtttgcccg ccgccattga aaaggcgctc gattttctgc
     2761 gcgctaccga tttcaacgcc ctggagccgg gcgttgtcga aatcgacggc aaaaatattt
     2821 atgcgcaaat tatcgattta accacacgcg aagcggtgga aaaccgtccg gaagtgcatc
     2881 gtcggtatat cgatattcag tttctggcgt ggggtgaaga gaaaatcggc attgctattg
     2941 atacgggaaa taataaagtc agcgaatcac tattagagca gcgcgatatt attttttatc
     3001 atgacagtga acatgaatca tttatagaaa tgataccggg cagctacgca attttctttc
     3061 cgcaggatgt tcaccgacca ggttgtattt tgcaaacagc ctctgagata cgaaaaattg
     3121 tggttaaagt cgcattaacg gcgctgaatt aatcgaataa tcgtctacgc cagaacgact
     3181 gggtaatgtt attgctctaa ctatattaat aatttaggtt gctgcacatg caacttgaat
     3241 tatgacagac atagcctcaa ggaatagcta tgaaaaaaaa tacttgaagc aatactggcg
     3301 attaatctcg ccgtactttc ctgtattgtg tttataaaca ttattttaag atatggtttt
     3361 cagacaagca ttttatctgt agatgaattg tcacgttatt tatttgtctg gttaacattt
     3421 attggcgcga ttgtggcttt tatggataac gcccacgttc aggtcacctt tctggtggag
     3481 aaactctccc ccgcatggca acgacgagtc gctctggtta cccattcctt aatcttattt
     3541 atttgtggcg cactggcctg gggcgcaacg ctaaaaacaa ttcaggactg gagcgattat
     3601 tcaccgatcc tcggtttacc catcggcctg atgtatgccg cttgtctgcc caccagcctc
     3661 gttatcgcat tctttgaact gcgtcattta tatcaactca tcacgcgtag caattctcta
     3721 acgtcgccac cgcaaggagc ttaatcatgg ctgtgctgat ttttctgggc tgtctgttgg
     3781 gtggtattgc tatcggtttg cctattgcct gggcactgtt gttgtgcggg gcggcgttaa
     3841 tgttctggct ggacatgttt gatgtccaga tcatggcaca aacgctggtg aacggagccg
     3901 atagcttctc cctgctggcg attccgttct ttgtgctggc gggtgaaatc atgaatgcgg
     3961 gcggcttgtc aaaacgcatt gttgacctgc cgatgaaact ggtggggcat aaaccaggcg
     4021 ggctgggcta cgtcggcgtg ctggcagcta tgattatggc gagcctttct ggttctgccg
     4081 ttgcggatac cgccgccgtt gccgctctac tggtgccgat gatgcgcagc gccaactacc
     4141 cggtcaaccg ggcggcgggg ctgattgcct ctggcggcat tatcgcgcca attattcccc
     4201 cttccattcc gtttattatc ttcggcgttt ccagcggatt atccatcagt aagctgttta
     4261 tggcgggcat tgcccctggc atgatgatgg gcgcaacgct gatgcttacc tggtggtggc
     4321 aggccagtcg ccttaattta ccccgccagc aaaaagcaac gatgcaggaa atctggcact
     4381 cttttgtctc cggtatctgg gcgctgtttc ttccagtcat cattattggc gggtttcgct
     4441 ccggtctgtt tacccctacc gaagccgggg ccgttgctgc tttctatgcg ctgtttgtcg
     4501 ccacagttat ttaccgtgaa atgaccttcg ccacactttg gcatgtactc attggcgcgg
     4561 cgaaaaccac ctcagtggtt atgtttctgg tggcctcggc acaagtttcc gcctggctga
     4621 ttaccattgc tgaactgccg atgatggttt ctgatttact gcaaccgttg gtcgattcac
     4681 cgcgtctgct gtttatcgtc attatggtgg ctatcctgat tgtcggcatg gtcatggatt
     4741 taacgccgac ggtattaatt cttaccccgg tattaatgcc tttagttaaa gaggcaggaa
     4801 tcgatccgat ttatttcggt gtcatgttta tcattaactg ctcaatcggc ttaattacac
     4861 cgcctatcgg taatgtgctt aacgttattt caggggtggc aaaactcaaa ttcgatgatg
     4921 cagtcagagg cgttttccct tacgtcctcg ttttatattc cttattagtc
//
LOCUS       DAZKIV010000229         4969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZKIV010000229 DAZKIV010000000
VERSION     DAZKIV010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.231"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..780
                     /locus_tag="ACHOT6_004503"
     CDS             <1..780
                     /locus_tag="ACHOT6_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFP9949593.1"
                     /translation="QSIANYISPMAQLWELASSRYHDAPERALSPWRGHRLDVAQSRE
                     SYEAFSNKELLQVLQVFSGNSAENKEMIALCLIGAYTGMRINEIASLTIDDVKEIEGV
                     LCFEITQGKTKAAARVVPVHSLITPLVLSLREKPHNGFLFYHASITERADGKRSTWHT
                     QRFTRAKRKALGEKGTERKVFHSLRHGVAQLLDRNQIPEDRIALLLGHTRGNTETFRT
                     YSKNAASPVELKKYIELLRYPEIEKGLSINKKSNLRRKTTP"
     gene            complement(862..1575)
                     /locus_tag="ACHOT6_004504"
     CDS             complement(862..1575)
                     /locus_tag="ACHOT6_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HFP9949594.1"
                     /translation="MATAGILTIRAANTPEQHVQAVYKAEQDINSTKNENSKANKTKG
                     ENGFAIVTSGLASSGNDVYENYMALAFDSVDDVNVRRSADVTSYPVENGATVSDHVQI
                     KNNKFSLKGRITETPIKSDPGLLKSAGVNGNRRSLAIDYLNQIMDSRQPFLLVTENKT
                     FENVVLTGIEYTEEASESLVFDLSFEQIRLVSYGTVNTVAIKTQPSKNIGANMKKRVN
                     TEKSSSEGEDTITPAFKQE"
     gene            complement(1610..3439)
                     /locus_tag="ACHOT6_004505"
     CDS             complement(1610..3439)
                     /locus_tag="ACHOT6_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949595.1"
                     /translation="MLEINTSKIKNTVTFSVDKDSLKKAKDSITGLKEFAENIKPAKL
                     RFDNVTKGYKKAQSEVDKIAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELK
                     LLDVSSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKIN
                     GNRKVQLAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTGEFMANSFANAETQGELI
                     QRARLGGVDVNQMYNITEWAYRNGVDSMMGDQGARKYLDQMKDVRERAAKSYSEAELV
                     KDKKTGKSEWKGGDNAINELLNIGVINKSDLKKFADNPAGLISKAVNGMMKKGFSDSQ
                     IGQRLEDLGDDLMLTSKYWQRSVKDVQESINQQKASGKWLTETQQDSIVKFRELNRQL
                     SQLSDARQVAFVDGFMKSLDPKVTEEFLKNLSNLTPYFTKLGEAVGSLFEAIMKIVNW
                     FNRNDDKTDAIQKNLGDAPPLSNEGMKQHLSNLVPDQYKGAGTATTTPDNSHSLFNTI
                     KGLLFDDNSSVSDVKMSVSEAPITNLKQGALNNLAMTTPAYNLSPVFNLNPTFEVVTE
                     VPLTINSDTSRLSDYVDFTARASRDSFLKSLTLTSLSGQSNGG"
     gene            complement(3458..3658)
                     /locus_tag="ACHOT6_004506"
     CDS             complement(3458..3658)
                     /locus_tag="ACHOT6_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001350140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949596.1"
                     /translation="MEVILKSKNGVHVHLDANDSRGLLNLKYLCSLLDVPYEGVKARM
                     FRLNESIDQALHHFLSKEGGKK"
     gene            complement(3745..4773)
                     /locus_tag="ACHOT6_004507"
     CDS             complement(3745..4773)
                     /locus_tag="ACHOT6_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9949597.1"
                     /translation="MILGNDYVDLAPLFQTHSTRNFLLSTLDFTDPVGVSSHKVAVSQ
                     LVESNESLFNKETSRFSSEHNVTKREQGKEWLIEIPYFLREDVIRPSDVQGKRKPGTD
                     FQETLTDIYADYVAKHHVAYQRTKESVLAASLFSGKTYTPKTDDVLIEWGKLFNVSAM
                     KATVNASSTDTTKIFKEFDQIATDMIAKAQKQAATIERVVVFCKPEAFSAIRFSAGMA
                     NAFQYVSPLEEGNVVYQRRDLLPGVTAFTIPGTNIDVVKLVDPLHLAHMTADAVAVPK
                     FAKGSNVYQNIYGAASSTFELINAAPAEVYSYSYESSRGDAVNVVTENSQMVVNHGVG
                     FSVQITVK"
     gene            complement(4789..>4969)
                     /locus_tag="ACHOT6_004508"
     CDS             complement(4789..>4969)
                     /locus_tag="ACHOT6_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949598.1"
                     /translation="VVLSEFVTAGENKPVDVLRSNAGLVCIKADALNAVSEAAKTALI
                     KKGFQLEGFHSVFTS"
BASE COUNT         1368 a         1066 c          975 g         1560 t
ORIGIN      
        1 caatcaattg caaattatat cagcccaatg gcccagctat gggagttagc ttcatcacgt
       61 taccacgatg caccagaaag ggcgctctcc ccctggcgag ggcataggct tgatgtggca
      121 caaagtagag agagctatga ggcattttct aacaaagagc tattgcaggt gttgcaagta
      181 ttttccggta attcagcaga aaacaaagaa atgatagctt tgtgtcttat cggtgcttat
      241 acaggtatgc ggatcaatga gatagcaagt ctcacaatag acgatgtgaa agagatcgaa
      301 ggtgtgctgt gttttgaaat cacacaggga aaaacgaaag ctgcggcacg tgttgtgcct
      361 gtgcatagcc ttatcactcc attggtgtta tctcttcgtg aaaagcctca caatggcttt
      421 ttgttctatc acgccagcat tacagagcgt gctgatggta aacgttccac gtggcacacg
      481 cagagattta caagagctaa acgaaaggct ttaggggaaa agggaacaga aaggaaggtg
      541 tttcattctc tgagacacgg agtagcacag cttcttgatc gaaatcaaat tccagaagac
      601 aggatcgccc ttctcctggg ccatacacgc ggcaatacag aaacattccg cacatatagc
      661 aagaatgcag cttctccagt agagcttaaa aaatatattg agcttctacg ctaccctgaa
      721 atagagaaag gcttatcaat caataaaaaa tcaaatttaa ggcgtaaaac aacgccatag
      781 acgcaataaa gaggctccaa catagattgg tagcctcttt tataattaca tcgttacaga
      841 gcgttttaga ggcgtttacg gctattcctg tttaaaggca ggagtaatag tgtcttcacc
      901 ttcactactt gatttttcag tgttaacacg ttttttcata ttagccccga tgttcttaga
      961 tggctgtgtt ttgatagcta cagtgttcac agtgccataa gaaactaatc ttatttgctc
     1021 aaagctaaga tcaaatacca gggattcgct tgcctcttct gtgtattcga ttcctgttaa
     1081 aacaacgttc tcaaaagttt tattttctgt aacaagaaga aaaggctgtc tactgtccat
     1141 aatctgattc aggtagtcga tagctaatga tcttctgttc ccgttcaccc ctgcactctt
     1201 taacaagcca ggatcgcttt taatcggtgt ttcagtgatc cttcctttaa gagaaaactt
     1261 attgttttta atttgaacgt gatcagacac agtagcacca ttttctacgg ggtagcttgt
     1321 aacatctgcc gaacgtctca cgttcacatc gtcaacacta tcaaaggcca gtgccatata
     1381 attttcataa acatcattgc cagaagatgc caagccactg gtaacaatag caaatccgtt
     1441 ctctcctttt gttttattgg ctttgctatt ttcgtttttt gtagagttta tatcttgttc
     1501 tgctttgtaa acagcctgaa cgtgttgttc tggagtattt gctgcacgta tggttaaaat
     1561 cccggcggtc gccataataa taattttcct tgtaatgtta tggcgggaat tacccgccgt
     1621 tggattgtcc tgataagctg gttaatgtta atgatttcaa gaagctatcc cgacttgctc
     1681 tcgctgtaaa atccacataa tcgcttaatc ttgatgtgtc tgaattaata gtaagaggaa
     1741 cctctgttac cacttcaaat gtaggattca gattaaacac aggagataaa ttataagccg
     1801 gagttgtcat tgcaagatta tttaaagcac cctgtttgag attggttatc ggtgcttcgc
     1861 tgactgacat ttttacatct gacacagaag aattatcatc gaaaagcaat cccttaatcg
     1921 tgttaaagag agaatgacta ttatcaggtg tagttgttgc agtgccagcg cctttatatt
     1981 ggtcaggaac gagattagaa agatgctgtt tcatcccttc attgcttaat ggcggtgcat
     2041 cacctaaatt tttctgaata gcatctgtct tatcatcatt acgattaaac cagtttacaa
     2101 tcttcataat agcttcaaag agacttccta cagcttctcc cagcttcgta aaatacggag
     2161 tgagattaga aaggtttttc aaaaactctt ccgttacttt tggatcgaga gatttcataa
     2221 agccgtctac gaatgctact tgtcttgcat cagaaagctg tgaaagctgt ctgtttagtt
     2281 ctctgaattt aacaatagag tcttgctgtg tttctgttag ccattttcca gaggcttttt
     2341 gctgattaat actttcttgt acatctttaa cggaacgttg ccaatatttt gacgtaagca
     2401 tcaaatcatc gcccaggtct tctaaacgtt gtccaatttg ggagtctgaa aacccttttt
     2461 tcatcattcc attaacagcc ttagaaatta atccagccgg attatcagca aattttttaa
     2521 ggtcgctttt gttaataaca ccgatgttta gaagttcatt aatagcatta tcaccgcctt
     2581 tccattcaga ttttccggtt ttcttgtctt tcaccaattc agcttcactg taggattttg
     2641 cggcacgttc cctaacatct ttcatctgat ccaggtattt cctagcccct tgatcaccca
     2701 tcatagagtc aacgccgttt ctataggccc attctgtaat gttgtacatc tggttaacgt
     2761 ctacaccgcc aagccttgca cgttgaatta gctctccttg tgtctcagca ttggcaaaac
     2821 tattagccat aaattcaccg gttttactaa ccgccatcac gccagcagca gccgtagcgc
     2881 ctccaaataa cagagcgccc atagtggcgg aattccctga tcctccctta acgggggcta
     2941 attgaacttt tctgttgcca ttaatcttac gttgttgttg ttgtaagcgt ttcatctggc
     3001 tattcatacg tgcaagcgag atagcccctt gctcatattg tttcgctatc tctctagcct
     3061 gtgcaatagc tttgtattgt tccgctacag agaggcggtg cattgcactg atagagcttg
     3121 acacatcaag gagctttaat tctgctttct ctttacgtgc tgctatttgt tgctgttgcc
     3181 ttgcctgtgc tcttgctgcg gcagcttgtg ctctttgatt ctgtttttct gttttggctt
     3241 tctgttgagc aatcttatct acttcgcttt gtgctttctt atagccttta gtgacattat
     3301 caaatcttaa tttagctggc ttgatgtttt ctgcgaattc ctttaagcct gtaatagagt
     3361 ctttcgcttt tttcaagctg tctttatcta cactaaatgt aactgtgttc ttaattttag
     3421 aagtgttaat ttctaacatt taaaatcccc ttattaatca ttttttacca ccttctttgc
     3481 ttaagaaatg gtgtagagcc tgatcaatac tctcgtttag tctgaacata cgggctttta
     3541 ctccttcata tggaacatct aagagagaac atagatattt cagatttaac aagcctctag
     3601 aatcattagc atctaaatgt acgtgtacac cattttttga tttcagaatc acttccatat
     3661 tttaatcacc tctcaaaatt taaaaataaa taaaaaaagg cgaagcgtgt tattcacgcc
     3721 acgcctctat tacacgttaa aatattattt aacagtgatt tgaacggaga agccaacgcc
     3781 gtggtttaca accatctggc tattctctgt aacaacgtta acagcatcac cacgagaaga
     3841 ttcatagctg tagctataca cctcagcagg agcggcattg atcagttcaa aggtgctgga
     3901 agcagccccg tagatgttct gatatacatt agagccttta gcaaacttcg gaaccgctac
     3961 agcgtctgcg gtcatatgtg caaggtgtag cggatctacc agtttaacaa catcaatgtt
     4021 agttcctgga atagtgaacg ctgtcacccc cggtagcagg tcacgacgct gatacacaac
     4081 gttcccttct tccagtgggc ttacatactg gaatgcgttt gccatacccg cagagaatcg
     4141 aattgcagag aaggcttcag gtttacagaa cacaacaaca cgctcaatgg ttgcagcctg
     4201 tttttgtgcc ttagcaatca tatccgtagc aatctgatca aattctttaa aaatcttcgt
     4261 ggtgtcagtg ctggaagcgt tcacagtggc tttcatagct gatacgttaa acagcttgcc
     4321 ccattcgata agaacatcat cagttttagg cgtgtaagtt ttaccgctaa acagagatgc
     4381 agccagcacg ctctcttttg tacgctgata cgctacgtgg tgtttagcaa catagtcagc
     4441 atagatatca gtgagtgttt cctgaaaatc agtgccaggc ttacgcttgc cttgaacatc
     4501 agaaggacgg atcacatctt cacgaagaaa atacgggatt tcaataagcc attctttacc
     4561 ttgttctcgc ttagtaacgt tgtgttcaga agagaaacga gaggtttctt tgttaaagag
     4621 agattcgttg ctttcgacaa gctgggaaac agctacttta tgagaactaa cgcccaccgg
     4681 atcagtgaag tcgagggtag agagcagaaa gttacgcgtg ctgtgtgtct ggaaaagcgg
     4741 agctaaatca acgtaatcat tacctagaat catttattat tccttttatt aagaagtgaa
     4801 aacagaatgg aacccttcaa gctgaaagcc ttttttaatt aatgctgttt ttgctgcttc
     4861 acttacagca ttcagggcat cagctttgat gcatacaagc ccagcattgc tacgtagcac
     4921 atctacaggc ttgttttccc cagccgttac aaattcactc agaacaaca
//
LOCUS       DAZKIV010000230         4908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZKIV010000230 DAZKIV010000000
VERSION     DAZKIV010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.232"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..2470)
                     /gene="traG"
                     /locus_tag="ACHOT6_004509"
                     /pseudo
     CDS             complement(1..2470)
                     /gene="traG"
                     /locus_tag="ACHOT6_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
     gene            complement(2467..3840)
                     /gene="traH"
                     /locus_tag="ACHOT6_004510"
     CDS             complement(2467..3840)
                     /gene="traH"
                     /locus_tag="ACHOT6_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraH"
                     /protein_id="HFP9949599.1"
                     /translation="MPRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNVQLISMTLPDINAGCGGIDAYLGSFSFINGEQL
                     QRFVKQIMSNAAGYFFDLALQTTVPEIKTAKDFLQKMASDINSMNLSSCQAAQGIIGG
                     LFPRTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKVSDKDKERVTKNI
                     NIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTDRSIIRAMMEGG
                     TAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVSDTPLDDKEKGF
                     ISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQARAMVATGNYD
                     EAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(3840..4202)
                     /gene="trbJ"
                     /locus_tag="ACHOT6_004511"
     CDS             complement(3840..4202)
                     /gene="trbJ"
                     /locus_tag="ACHOT6_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HFP9949600.1"
                     /translation="MLPVLWRGWIPFCRCTEGKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIHQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRRTGRKYRD
                     IPAEMSYAPGEQIKGGQE"
     gene            complement(4132..4677)
                     /gene="trbB"
                     /locus_tag="ACHOT6_004512"
     CDS             complement(4132..4677)
                     /gene="trbB"
                     /locus_tag="ACHOT6_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HFP9949601.1"
                     /translation="MSLTKSLLFTLLLTAAAVQASTRDEIERLWNPQGMATQPAQPAA
                     GTSARTAKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPLDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     AGFMARMDTVLQMYGGKKGAK"
     gene            complement(4664..>4908)
                     /gene="traQ"
                     /locus_tag="ACHOT6_004513"
     CDS             complement(4664..>4908)
                     /gene="traQ"
                     /locus_tag="ACHOT6_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HFP9949602.1"
                     /translation="TGVWVFSLGLWFHIVARLVYRKPWMAFFLAELIAAILVLFGAYQ
                     ILDTWIARVSREEREALEARQAAILAAEKESRHVSH"
BASE COUNT         1149 a         1370 c         1213 g         1176 t
ORIGIN      
        1 ctccattcct ttaccaatat caccacggga ttcccgccag gcattatcaa caccaggctg
       61 tacctgttcc tgcacaaaag accaggccat cgcccgacga cgttctgcaa tctccggtga
      121 actggtgttt gtcagaatag cttctgcatc ctgtggcgcg tgtttcatta catactgtgc
      181 aaactgttgc gacaagtctt cactcatctg cccactcatg ctttcagtac gggatgccat
      241 ttcagcatat tcatggctgc gggtcatatt cgtcgtgtac tggtcgtaac tctgcttcgc
      301 tgaattcagg gctgcagaca actgatccac acgggaatca gcattattgt cagtatggct
      361 gccggactca ctgaccttgc gactggtaaa gtaatcgctg gcctctttaa agtcttgtgt
      421 agctctggca tcgatatcat gacgagcatt atggctcgac tgagtattac tacttgccgt
      481 atgcgcatca ttatcttccc aatcaatacc agcccgacca ccacctctaa cgcctaatcc
      541 tccaccaaca ccaagaattt taggtttaac cccccattca gcatgagcat ctccatacat
      601 cccggcagaa gctctcgtac ttctggatgc cagttcctga gtcgcctgct cattactgat
      661 attatgcgcc ttcgcatagc tttccactgc actgcgcata cggctggcca tcatggagtc
      721 ctgtgcgctc atcgtgctgt cagcaccacc tgtgacagaa tcactgctcc cccggttaga
      781 accaaactga ctgagtgagt gagttccagg cactggcaat actgctgctg aacccatgca
      841 gggcgctttc tgctctgttc gacgcctccc gggccatttc ctgttgtgcc gccgcaatct
      901 gacgcgttgc attgatgccg accggtaacc gggacatcgc cccgctcgca tccatcacca
      961 tattaccgtc acgggtctgt gtggcagttg cgccactgcc ggtctggtac atcatctgac
     1021 caaacgacgt ggtgctgttg gtgctccagc tgaagccgtt cacgttttcc gtctgcatgt
     1081 tgccgtagga gtaattaccg tcaaccacac tacctgccgc actggcagtc gggctgatag
     1141 cagaggaagc gaaatggctg tacacactgg aaaatccggc cccgagtccc ctgaccatca
     1201 tccaggataa tggcgggatc atcatggaaa gatacccggc agtggaagcc agatcggagt
     1261 atttcagctg tatctgtgaa atttccgaga gcaccaccgg tgcaccattc tgcttcgcat
     1321 aaaatgtcat ggcgctgttc agaatggcgt acaacattgg ccatgactgc aaccacatca
     1381 gggcaaacac atagcctttc aggacggaca gcgtcagttt gttgaagaca gctgccagta
     1441 ccaggagcgg gaaaataccg atggctatcc ccgtcaggat ggtctgcgtc atgggcagtg
     1501 tccgcatcgc cacatgaccg atggagacat gtgccagacg ttgcttctcc atcgatgatg
     1561 tggtggccag attcaccagg ctggccgaat caccattacg tgcggcataa ctggtgatcc
     1621 cctccttcag cgcattgatg gtgacattct ggcggatgat ctggctggct gatttacttg
     1681 agccatagaa gtaactgtaa ctgtcgccaa tcagcgtact gaacaacagg ttcggatcag
     1741 ggcgaccacc aaataactgc tgggcataat aatgccaggt ttttccgcca ctctgtgtgt
     1801 ccagattcag cttgtctttc agcgaaaccg acgcgtcctt acaggtcacg aaattattat
     1861 tgctgtcgta aacgccccgc agcggactgg ggcgggaaaa aatcagcgtg tagggatcag
     1921 cggaggccat cagctcctcc agcgtgtatt tgtggttcag gtaaatatcc cccatcacac
     1981 agttctgcac ataatcctga aaaagattgg cgatttccgg attgcgggac aaaaagtcgg
     2041 tgctttttga taccagttct gcgccgaaca gcatccccgt tttgctgtag gtgacactgt
     2101 ccggctgggt gaatatcatc tcgtaactgg ccaccattgc atgcccgata cgggtcgtca
     2161 gtgaaagtgg catcgccagt ccgaccggca cattatccac ccggtgtact ttgaccaggt
     2221 cactgttatc aataatctgc accgatgtgc ggacattaac cagcaggctg ataagcacaa
     2281 acacggccac ccagcccagc aaatccatca cgttgtgccg ctgtacccac atcacggcca
     2341 ccgcgagaac agacaatgtg agcgcaattt tttcaatgga atcccacgtc ctggttccca
     2401 taaaggcggc aatggcgttc aggttattcc gcaaccactc accaccggca atcacataaa
     2461 cttcattcac agcgtgcttc ctccgaagtg atagttgttc tggtaacgac tgagcatgcg
     2521 ggcggaaagc tgctgacgca tgtagctcat ctgacgatcg acaaccagca gcgcatcctg
     2581 ctgtacctgc acctgtgact gaaacgccgc aatctgccgg gtggcatcat tcatgttgtc
     2641 gttaatatgt tcgataactg cttcgtcata atttcctgtg gccaccatcg cccgggcctg
     2701 ctgtatcagc tcctgaatgt actgcagcag gatgtcgtaa ccgatatagt ccgtcagctg
     2761 gtaaatcata ctgttggaaa caccgagcat ctgcgggtca accaggtatt tgaagacggg
     2821 gatggtggta ctggaaataa agcctttttc cttgtcatcc agaggcgtgt cactgacagc
     2881 cttgttctga atgctggcca gaagtttagt aatctgagac ttcagtgcat tatcccggct
     2941 gatggtcacc ggtgtgtctg ccaccacttt caggcattta tcagaatcgt tgcagtgata
     3001 aatttttgct gtgccgcctt ccatcatggc ccggataatt gagcggtcag tggttctggc
     3061 tgacagcggt gtgatttcgc cgttgggacc aaacaccagt gagccggtca gcgtcatcac
     3121 aaactctttc agctctttgt tgccgtcaaa cattctgttt ttggaaagcg cattccacat
     3181 gatgttgatg tttttggtca cccgctcctt atccttgtcg ctgactttat ccctgacgct
     3241 gtcagatttc ccgccaacgg tacatccctg ccgggaagcc gcccagtcag caaaaatatt
     3301 gctctcaccg gcaatgtcct gacagacttt ctgctgggac acctgcgtcc gggggaagag
     3361 cccgccgata atcccctgtg ccgcctggca ggaactgagg ttcatactgt taatgtcgct
     3421 tgccattttc tgcaggaagt cttttgcggt tttgatttcc ggcaccgttg tctgcagggc
     3481 aaggtcaaaa aagtaaccgg cagcattgct cataatctgc ttaacaaaac gctgcagctg
     3541 ctcgccatta ataaaactga acgagccgag ataggcgtcg atgccgccac atccggcatt
     3601 gatatccggc agcgtcatgg aaatcagctg aacatttttg acctgagtac gggcatacag
     3661 ggagccaccg taagcataac cggcggcctg cccctgccag acgcccggct gtgtggtatt
     3721 ggaggcgaag cccagcttgt taaagaactg attcatgtcg ctgttcacat ccgctgacgc
     3781 tgcgggtgtg accgtcagta aagcagcaca aagaaccaga agaggtttaa tgcgtggcat
     3841 cattcttgcc ctcccttaat ttgttctccc ggcgcatacg acatttcagc aggaatatcc
     3901 cgatatttcc gtccagtcct gcggctgtaa tatatttcag acaccgaaat ccatcccaca
     3961 attgccccca caaaaaacac aacagaaaca gaaaaggtaa ggcacagcga aacgacaatt
     4021 tctacagggc tggcgggata aaaaagcccc tgatggataa aggggatatt caggctgacg
     4081 attattcccg tcgcgatgcc ggatattccg gcaatgagta acaccacctg cttatttcgc
     4141 accttttttc cctccgtaca tctgcagaac ggtatccatc cgcgccataa aaccggcagc
     4201 atccgttgcc ccctgtaaaa gcggtaatgc ctcaagcgtg ttgacgttga ccaggaaggt
     4261 ggtcggtgtg gccaccggga tattcgggaa gaaggtctgc atcacgtcca gtggcaccgg
     4321 taatgcttcc ggaaaggcgg tatccccctg accatccagg gtgtagggaa aaacggaaaa
     4381 gccgtactgc tgtgccagct gtttcagtac cgggtcaaac tggtgacagt aagggcaatg
     4441 cccctgcata aacagcacca ccttccagtc ggccaggtta acctgcctgc cattactgag
     4501 gcggaaccag cggggagccg gctttgctgt cctggctgac gtgcctgctg ccggttgtgc
     4561 aggctgtgta gccataccct gcggattcca gagtcgctct atttcatccc gggtggaggc
     4621 ctgcacagca gcggctgtca gtaacagggt gaacagcagt gatttagtga gagacatgtc
     4681 tgctctcctt ttctgcagca agaatggcgg cctgacgggc ttccagtgcc tcacgctctt
     4741 cccggctgac gcgggcaatc cacgtatcca gtatctggta tgcgccaaac agaacgagaa
     4801 tggccgcaat cagttctgcc agaaaaaaag ccatccaggg ttttctgtaa accagacggg
     4861 caacgatatg aaaccagagc cccagggaga acacccatac acctgtaa
//
LOCUS       DAZKIV010000231         4895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZKIV010000231 DAZKIV010000000
VERSION     DAZKIV010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.233"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..478)
                     /locus_tag="ACHOT6_004514"
     CDS             complement(14..478)
                     /locus_tag="ACHOT6_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001253184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949603.1"
                     /translation="MRRKREEIASPEATQRLRAIWDAKKRELKLTQELAAELMGFEAQ
                     SAVSHYLNGKAPLNTDAALKFAVLLRVKPEELRPDLADLMNYVRSSGTYDESFEGEGW
                     RMVNKQQADLLDLFDILPESEKAKLIERLKGQNELYKEAFENMLAAQKRMKK"
     gene            583..858
                     /locus_tag="ACHOT6_004515"
     CDS             583..858
                     /locus_tag="ACHOT6_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9949604.1"
                     /translation="MTLDEYLKKNRVRQSCLAALAGCSQSMISLVATGRSQLSPEKVL
                     RIAEATNFEVTPHELRPDIYPNPTDGLPVGCKANTQNAQELIHENQA"
     gene            842..1264
                     /gene="ydaT"
                     /locus_tag="ACHOT6_004516"
     CDS             842..1264
                     /gene="ydaT"
                     /locus_tag="ACHOT6_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9949605.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAIIPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSEDSVIVH"
     gene            1277..2134
                     /gene="ydaU"
                     /locus_tag="ACHOT6_004517"
     CDS             1277..2134
                     /gene="ydaU"
                     /locus_tag="ACHOT6_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1376 domain-containing protein"
                     /protein_id="HFP9949606.1"
                     /translation="MLFVLILSHRAASYGAIMAALPYMQLYIADYLADTMHLSAEEHG
                     AYLLLMFNYWQTGKPIPKNRLAKIARLTNERWADVEPSLQEFFCDNGEEWVHLRIEED
                     LASVREKLTKKSAAGKASVQARRSRKEADVQKKQERNLTGVQTDVEVVFEHDVNTKAT
                     NKDTDKDLKTDPPLNPPRGNRGVKKFDPLDIALPNWISVSLWREWVEFRQALRKPIRT
                     EQGANGAIRELEKFRQQGFSPEQVIRHSIANEYQGLFAPKGVRPETLLRQVNTVSLPD
                     SAIPPGFRG"
     gene            2141..2887
                     /gene="ydaV"
                     /locus_tag="ACHOT6_004518"
     CDS             2141..2887
                     /gene="ydaV"
                     /locus_tag="ACHOT6_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9949607.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFSFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVNFNWESWRPNVGQPGIAK"
     gene            2909..3679
                     /locus_tag="ACHOT6_004519"
                     /pseudo
     CDS             2909..3679
                     /locus_tag="ACHOT6_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
     gene            3695..4087
                     /locus_tag="ACHOT6_004520"
     CDS             3695..4087
                     /locus_tag="ACHOT6_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9949608.1"
                     /translation="MAKVFTPEEREEVKARIVEFVRLSGRETFRQLADKTGVSKTAIR
                     CLSGALAASGDVWLSGCGVFPSEQAYRVWRKTPEKAADPTLIRKLPDGEIRRYNRRQN
                     IICRECRQSEVMQRVLAFYRGNFQEAVL"
     gene            4144..4728
                     /locus_tag="ACHOT6_004521"
     CDS             4144..4728
                     /locus_tag="ACHOT6_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9949609.1"
                     /translation="MTTFTKEQLISHVSENVKAMKFAVKQTAFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     KKGSSTNNFREISETSTNYPVTLDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRDGDEFFETQSRNVTDWMPLPEPPQEVRQ"
BASE COUNT         1304 a         1056 c         1344 g         1191 t
ORIGIN      
        1 gagatggtgg gagctatttc ttcatgcgtt tttgagctgc cagcatattt tcaaatgctt
       61 ctttgtagag ttcattctga cctttaagtc tttcgattag ttttgctttt tctgattcgg
      121 gaaggatatc aaaaagatct agtaaatcag cttgttgctt gttaaccatc cgccaacctt
      181 cgccttcgaa actttcgtca taagttcctg aggaacggac gtaattcatc agatcagcta
      241 aatcaggcct caactcttca ggcttaactc ttagtaatac agcgaatttt aatgccgcat
      301 cagtattaag tggagccttt ccgttgagat aatggctaac cgcagattgc gcctcaaaac
      361 ccattagctc ggcagcaagc tcctgagtca gtttgagctc tctttttttt gcatcccaga
      421 ttgcgcgcag acgttgcgta gcttccggcg atgcgatttc ttcgcgtttt cttctcatac
      481 caccatctta tgaatacagt tcataatctc aaactgatat aggtattgat catttaaatt
      541 agtatggtta atatttttgt gagcattact aaggtgacca ttatgacatt agatgaatat
      601 ttgaaaaaaa atcgtgtacg acagtcttgt ttggccgcgc tggctggttg ttcgcaatca
      661 atgattagcc tcgttgctac tggacgtagt cagttaagcc ctgaaaaggt attgcgtatc
      721 gcagaggcta cgaatttcga ggttacacct catgaactcc ggcctgatat ctaccctaat
      781 ccgaccgatg gtttacctgt tggatgtaag gctaacacgc aaaatgcaca ggagttgatt
      841 catgaaaatc aggcatgagc acatcgaatc agtgttgtta gccctggcag ccgaaaaagg
      901 gcaggcgtgg gtcgctaacg caattactga agaatatctg cgccaggggg gcggcgaatt
      961 gcccctggta ccaggcaagg actggaacaa tcagcagaat atctatcacc gttggttgaa
     1021 aggtgaaacg aaagcgcaaa gggaaaaaat tcagaaactg atccctgcgg ttctggcaat
     1081 tattccgcgc gagctgcgtc accgactctg catcttcgat accctggaac gccgtgcatt
     1141 actggcggcg caggaagcgt tgagtacggc aattgatgcg catgatgatg cagtccaggc
     1201 cgtttaccgg aaagcgcatt tcagcggcgg cgggtcttcc gaagattctg tcattgttca
     1261 ttaagcaaaa gtttccatgc tgtttgtgct tattctaagc caccgggcag catcatacgg
     1321 ggcaattatg gccgcattac catacatgca actgtacata gctgattact tggctgacac
     1381 catgcatttg tcagcagagg agcatggtgc gtatttgttg ctgatgttca attactggca
     1441 aacaggaaag ccaataccta aaaacagact ggcaaaaatt gcccgtctga ctaacgagcg
     1501 atgggctgat gttgaaccat ccttgcagga gtttttttgc gataacggcg aggaatgggt
     1561 gcatcttcgg attgaggaag atctggcatc agtcagggaa aaattaacca aaaaatcagc
     1621 cgcaggaaaa gcatctgttc aggccagaag aagcagaaag gaagcagatg ttcaaaaaaa
     1681 acaagagaga aatttaacag gtgttcaaac agatgttgaa gtggtgtttg aacatgatgt
     1741 caacacaaag gcaactaata aagatacaga taaagatcta aaaacagatc cccccctaaa
     1801 tcccccccgg gggaatcgag gtgtcaaaaa gtttgaccct ctggatattg ctttgccgaa
     1861 ctggatttct gtctcgcttt ggcgtgagtg ggttgaattt cgccaggcat tgcgtaaacc
     1921 gattcgaacg gagcagggcg ctaacggggc gatacgggag ctggaaaaat tccgccagca
     1981 gggtttttca cctgagcagg tgattcgaca cagcatcgcc aatgaatacc agggcttgtt
     2041 cgcgccgaaa ggtgttcgac ctgagacgtt actccgacag gttaacaccg tttcgctacc
     2101 ggatagtgcg atcccgccag gcttcagggg gtaacagacc atgaaaaata ttgcgacagg
     2161 cggcgttctg gagcgtatcc gcagactgac cccaccacat gtaaccgccc cattcagaac
     2221 ggttgcggag tggcgtgagt ggcaacttgc tgaaggacag aaacgttgcg aggagatcaa
     2281 ccgcctgaat cgtcagttgc gggtggaaaa aattctgaat cgctctggca tccagccgtt
     2341 gcaccgtaaa tgctcgtttg cgaattacca ggtgcagaac gacggccagc gatacgcgtt
     2401 aagccaggcg aaatccatcg ccgatgaact gatgaccggg tgtacaaatt tttcgttcag
     2461 cggaaaacct ggtaccggga agaatcactt agcggcagct atcgggaatc gcctgctgaa
     2521 agacggtcag acagtgattg tggttaccgt ggctgatgtt atgagcgccc tgcacgccag
     2581 ctatgacgac gggcagtcag gcgaaaaatt tttgcgggag ctgtgcgaag tggatctgct
     2641 ggttcttgat gaaattggca ttcagcgcga gacgaaaaac gagcaggtgg tgctgcatca
     2701 gattgttgat cgccggacag cgtcgatgcg cagcgtgggg atgctgacaa acctgaacta
     2761 tgaggccatg aaaacattgc tcggtgagcg gattatggat cgcatgacca tgaacggcgg
     2821 gcgatgggtg aattttaact gggagagctg gcgtccgaat gttggtcagc caggaattgc
     2881 gaagtaattt ttgttggagg acgttttaat ggaaactgta tttgacgcac tgaaagcaat
     2941 gggaaaagcc acgtcggtag aactggctgc gcgacttgat atcagtcgtg aagaggctgt
     3001 caacgagctg tgggaactca aaagaaatgg cgtcgttgat aaaactggtc acacctggtc
     3061 tctggctggc gaaggtgaat cccgggtaac cgaagagcgg ccagtaaaat ctgaagcaca
     3121 ggatatgctg accggggagg tcgaacaaaa agttaccgca gacatgatga ttgagtttat
     3181 cggtcaggat ggggctaaaa cgtgtgagga actggcgggt aagttcggtg tcagtactcg
     3241 caaggttgct tccacgctgg cggtggtaac cgcaacgggg cggctggcac gcgttaatca
     3301 gaacggtaaa tttcgttact gcatgccggg cgataattta ccagcatagc cgaaagccgc
     3361 gctggtaacg gaaagtgatg gtaaggcctt tcctcagcca gcaggtgctg cgttaccagt
     3421 ccgggaagcc gcaacacagg aagaaattaa aacagaaact gtggcggaca ttgtgcagcc
     3481 gttgccatcg tttaccgaaa cgcaagcaga tgagctgatt tttccgtccc ttcgcagggc
     3541 aaacctggcg ctgcgcaggg cgaaaagtga tgttcagaag tgggagcgag tctgcgccgc
     3601 gctgcgggag ctgaacaagc accgggatat tgttcgacag attactgatt cttcccgccg
     3661 tgttgtatcg gaaaagtgat tgccggaggc gcttatggca aaagtattta caccagaaga
     3721 gcgggaagaa gtgaaggcgc gcattgtgga attcgtgcgc ctgagcggac gagaaacttt
     3781 tcgacaactg gcagataaaa cgggtgtcag taagaccgct attcgttgtt tatctggtgc
     3841 gcttgcggcc agtggtgatg tctggctctc tggttgcggg gtatttccat cagagcaggc
     3901 gtatcgcgta tggcgtaaga caccggagaa ggctgctgac ccgacactga ttcgaaagtt
     3961 acctgacgga gaaatacgtc gttacaacag acggcagaac ataatttgtc gtgagtgccg
     4021 ccagagcgaa gttatgcagc gtgtgctggc gttctatcgg ggaaactttc aggaggcggt
     4081 actgtgagtg aattagctat caggcttcaa ttgtcgctgg cattcgcatc aaaggagaat
     4141 gagatgacca cttttacaaa agagcagtta atcagtcatg ttagtgaaaa tgtaaaggcg
     4201 atgaaatttg cagtaaaaca gacagcattc aaaaattctc tcgaggcaat tgagttggat
     4261 ttagcactgg cccttgttgc tcaggcttcg ctggaagcag agcccgtgct ttatatgaat
     4321 cgatttaccg gaaagacatt ctcactggaa gagcaacccg gtgctgataa ggaaccggaa
     4381 atatacgtgc cgctatatgc tgccccgcca gacagcgccg ccatgcttca ggctggaaac
     4441 tttcgggaaa agaagggttc gtcaaccaat aattttcggg aaatctcgga aacgtcaacc
     4501 aactatccgg taactctgga tggttggata agctgtagtg agcgaatgcc ggatgacggt
     4561 cagcacgtaa ttattttatg tgatggcgca ttcgttcttt atgcgcaata tcgagacggt
     4621 gagttttttg atgtagtccg tgatggtgat gaatttttcg aaacacagag ccgcaatgta
     4681 accgactgga tgccgctacc agaaccgccg caggaggtgc gccaatgaac tggcctgaag
     4741 catttgcaat tacaggcgtt gctatggcta tcgctttttt agtatatgtt atttgtcggt
     4801 gggggtaaaa acgttcgccg ggattaacac caaaggaggg aatatgtcgg atgatatttc
     4861 actggcaatg gaaggtgcgc tggctgttat tgctg
//
LOCUS       DAZKIV010000232         4827 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZKIV010000232 DAZKIV010000000
VERSION     DAZKIV010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.234"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..2004
                     /gene="mngA"
                     /locus_tag="ACHOT6_004522"
     CDS             88..2004
                     /gene="mngA"
                     /locus_tag="ACHOT6_004522"
                     /EC_number="2.7.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HFP9949610.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            2022..4655
                     /gene="mngB"
                     /locus_tag="ACHOT6_004523"
     CDS             2022..4655
                     /gene="mngB"
                     /locus_tag="ACHOT6_004523"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFP9949611.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         1181 a         1129 c         1339 g         1178 t
ORIGIN      
        1 tttcaatcaa gtgaaattga tcacataatg gtattgtttt atcgggcaca ctggcgcgac
       61 tataaaaacg atcaagtgag gatcatgatg aatctgacga ctctgaccca ccgcgatgcg
      121 ttgtgtctga atgcgcgctt taccagccgt gaagaggcca tccacgcgtt gactcaacgt
      181 cttgctgctc tggggaaaat ttccagtact gagcaatttc tggaagaagt gtatcgccgt
      241 gaaagccttg gcccgaccgc cttaggtgaa gggttggctg tgccgcatgg caaaactgct
      301 gcggtaaaag aagcggcgtt tgcggtcgcc acactcagcg agccgcttca gtgggaaggc
      361 gttgatggcc cggaagcggt tgatttagtg gtgctgctgg cgattccccc caatgaagcg
      421 ggtactacgc atatgcaact gctgacagcg ctgaccacgc gcctggcgga tgatgagatt
      481 cgggcgcgta tacagtcggc gacgacgcct gatgagttgc tctcggcgct ggatgacaag
      541 ggaggcacgc aaccttctgc ctctttttcc aacgcgccaa ctatcgtctg cgtaacggcc
      601 tgtccggcgg gtattgctca cacctatatg gctgcggaat atctggaaaa agccggacgc
      661 aaactcggcg taaatgttta cgttgaaaaa caaggcgcta acggcattga agggcgttta
      721 acggcggatc aactcaatag tgcaaccgcc tgtatttttg cggctgaagt cgccatcaag
      781 gagagtgagc gttttaatgg cattcccgcg ctttcagtgc ctgttgccga gccgattcgc
      841 catgcagaag cgttgatcca acaagcgctt accctcaagc gtagcgatga gacgcgtacc
      901 gtacagcaag atacgcaacc ggtgaaaagt gtcaaaacgg agctgaaaca ggcactgttg
      961 agcggaatct cttttgccgt accgttgatt gtcgcggggg gcacggtgct ggcggtcgcg
     1021 gtattactgt cgcaaatctt cgggctacaa gatctgttta atgaagaaaa ctcctggctg
     1081 tggatgtacc gcaagctggg cggcgggctg ctcggaattt tgatggtacc ggtgctcgcg
     1141 gcctataccg cctattctct ggcagataaa ccggcgttag cgccaggctt tgcggctgga
     1201 cttgccgcca acatgatcgg ctccgggttt ctcggcgcgg tcgttggcgg attgatagcc
     1261 ggttacttga tgcgctgggt gaaaaatcac ttgcgtctta gcagtaaatt caatggattc
     1321 ctgacttttt atctctaccc ggtgctcggt acgttgggag cgggcagtct gatgctgttt
     1381 gtggtgggag aacctgtcgc ctggatcaat aactcgctta ccgcctggct gaacggtctg
     1441 tcaggaagta acgcgctgtt gctgggtgcc attctcggtt ttatgtgttc ctttgacctt
     1501 ggagggccag tgaacaaagc cgcttatgca ttctgcctag gcgcaatggc gaacggcgtt
     1561 tacggcccgt atgccatttt cgcctccgtc aaaatggttt cggcatttac cgtaaccgct
     1621 tccacgatgc tcgcaccgcg cctgtttaaa gagtttgaaa ttgagaccgg gaaatccacc
     1681 tggctgttag ggctggcagg tattaccgaa ggggcgatcc cgatggcgat tgaagatccg
     1741 ctgcgggtta ttggttcgtt tgtgctgggc tctatggtaa cgggcgctat tgtcggtgcg
     1801 atgaatatcg gcctttcgac acccggtgcc ggcattttct cgctcttttt acttcatgat
     1861 aatggcgcgg gcggtgttat ggcggcaatt ggctggtttg gtgcggcatt ggtgggggct
     1921 gcaatctcga ctgcaattct cctgatctgg cggcgtcacg cggttaagca tggcaactat
     1981 ctgactgatg gcgtaatgcc ataaacaaaa aggaaacgac gatgaaagcg gtatctcgcg
     2041 ttcacatcac cccgcatatg cattgggatc gggagtggta tttcaccacc gaagagtcac
     2101 gcattctgct ggtcaataat atggaagaga tcctgtgccg actggaacag gacaacgaat
     2161 acaaatatta cgtactcgac gggcaaacgg cgatcctcga agattatttc gcggtgaaac
     2221 cggaaaacaa agaccgtgtg aagaaacagg tagaagccgg caagttgatt atcggcccct
     2281 ggtataccca gaccgatacc acgattgttt ctgcggaatc catcgtccgt aatctgatgt
     2341 acggaatgcg tgactgcctc gcgtttggcg agccgatgaa aataggttat ttaccagatt
     2401 cctttggcat gtccgggcaa ctgccgcata tctacaatgg atttggcatt acccgcacca
     2461 tgttctggcg cggatgttcg gagcgccacg gtactgataa aaccgagttt ttgtggcaaa
     2521 gcagtgacgg tagcgaagtg acggcgcagg tgctgccgtt gggttatgcc atcggtaagt
     2581 acttacctgc cgacgaaaac ggattacgca aacgcctcga cagttatttt gacgtgctgg
     2641 aaaaagcgtc tgtaaccaaa gagattttat tgccgaatgg tcatgaccag atgccattgc
     2701 agcaaaatat cttcgaagtg atggagaaac ttcgcgaaat ttatcctcaa cgaaagtttg
     2761 tgatgagccg ctttgaagag gtattcgagc agattgaagc gcagcgtgaa agtctggcga
     2821 ccctgaaagg tgaatttatt gatggcaaat atatgcgcgt gcatcgcacc atcggttcca
     2881 cgcgcatgga tatcaaagtt gcccacgcac gtattgaaaa taagattgtt aatctgctgg
     2941 aaccgctggc aacgctggcc tggacgttgg gttttgaata ccaccacggc ttgctggaga
     3001 aaatgtggaa agagatctta aaaaatcatg cccacgacag tatcggttgt tgttgcagtg
     3061 acaaagttca tcgcgaaatt gtcgcccgct tcgaactggc tgaagacatg gcggataatc
     3121 tgctgcgttt ctacatgcgc aaaattgccg ataacatgcc acagagtgat gccgacaaac
     3181 tcgtgctgtt taacctgatg ccctggccgc gggaagaagt tattaacacc actgtgcggc
     3241 tgcgcgccag ccagtttaat ctacgagacg atcgcggtca gcctgtaccg tattttattc
     3301 gccatgcccg tgagatcgat ccaggcctaa tcgatcggca aatagttcat tacggtaatt
     3361 acgatccctt tatggagttt gatatacaga tcaaccagat tgtcccttct atgggctatc
     3421 gcacgcttta tatcgaagcg aatcagcctg gcaacgtgat tgcggcaaaa agtgacgctg
     3481 aagggatact ggaaaatgct ttctggcaaa ttgcgctcaa tgaggatgga acgctacaac
     3541 tggtagataa agatagcggt gtgcgctatg accgggtgtt gcaaattgaa gaaagctctg
     3601 atgatggtga tgaatatgac tattcacccg caaaagaaga gtgggtaatg acttctgcaa
     3661 cagcgaaacc gcaatgcgag attactcatg aggcctggca gagcagggct gttatccgct
     3721 atgacatggc ggtgccgctc aatttgttgg aacgaagcgt ccggcaatcc acgggcagag
     3781 taggggtgga gatggttgtc actctgagtc ataacagcag acgtattgat gtggatatca
     3841 accttgataa ccaggctgac gatcatcgcc ttcgcgtcct gatccctaca tcttttaaca
     3901 ccgatagtgt tctggcagat acccagtttg gttcgctaac gcgccccgtg aacgacagtg
     3961 caatgaacaa ctggcagcaa gaaggctgga aagaagcgcc ggttccggta tggaatatgc
     4021 tcaactatgt tgccttacag gaagggcgta acggcatggc tgtctttagc gaagggttac
     4081 gtgaatttga agtcatcggt gaagagaaga aaacctttgc cattacgttg ctgcgtggcg
     4141 tgggcttact gggcaaagaa gatctgcttt taaggcctgg gcggccttcg ggaattaaaa
     4201 tgccagtccc ggactcacaa ctacgtggtc tgctttcttg tcgcctaagt ttattgagtt
     4261 ataccggtac gccaaccgcc gctggtgtag ctcagcaggc gcgagcatgg ctgactccag
     4321 tacagtgtta caacaaaatc ccatgggatg cgatgaagct caacaaagcc ggattcaacg
     4381 tgccggaaag ttatagtttg ttgaaaatgc ccccagtggg atgcctgata agcgcactta
     4441 agaaagctga agaccgacaa gaagtgattt tacggctgtt taatccggct gaatcagcaa
     4501 cctgtgatgc gactgttgct ttcagtcgcg aggtgatttc ttgctcagaa acgatgatgg
     4561 atgaacacat taccaccgag gaaaatcaag gttcaaatct atcggggcct tttttacccg
     4621 gccagtcacg gacgttcagt taccggcttg cctgaatagc aatcaaaccg aagccacata
     4681 tgcgcggcca gattgttgac aaagggcgct ttgttcatgc cggatacggc atgaacgctt
     4741 tattcggtct acaaaagcag gcaaattcaa tatattgcag agatgatgta gacactggca
     4801 agcgtagcgc atcaggtaat tttgcgt
//
LOCUS       DAZKIV010000233         4825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZKIV010000233 DAZKIV010000000
VERSION     DAZKIV010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.235"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            158..1132
                     /gene="hemB"
                     /locus_tag="ACHOT6_004524"
     CDS             158..1132
                     /gene="hemB"
                     /locus_tag="ACHOT6_004524"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFP9949612.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1238..2089)
                     /gene="tauD"
                     /locus_tag="ACHOT6_004525"
     CDS             complement(1238..2089)
                     /gene="tauD"
                     /locus_tag="ACHOT6_004525"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFP9949613.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2086..2913)
                     /gene="tauC"
                     /locus_tag="ACHOT6_004526"
     CDS             complement(2086..2913)
                     /gene="tauC"
                     /locus_tag="ACHOT6_004526"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFP9949614.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2910..3677)
                     /gene="tauB"
                     /locus_tag="ACHOT6_004527"
     CDS             complement(2910..3677)
                     /gene="tauB"
                     /locus_tag="ACHOT6_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFP9949615.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3690..4652)
                     /gene="tauA"
                     /locus_tag="ACHOT6_004528"
     CDS             complement(3690..4652)
                     /gene="tauA"
                     /locus_tag="ACHOT6_004528"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFP9949616.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            4630..>4825
                     /locus_tag="ACHOT6_004529"
     CDS             4630..>4825
                     /locus_tag="ACHOT6_004529"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949617.1"
                     /translation="MLRDEIAMIVCSPVLLCEQYSGIKTFIKRISRYRTDDFILSKKT
                     AKERLNMQLLRSNNWENYYTS"
BASE COUNT         1105 a         1395 c         1235 g         1090 t
ORIGIN      
        1 ttttcagtga atatgaaatt attgctattt ctattacaaa gatgtcctgg tctctctttc
       61 attctcttct tgtcaacatc acgacaactt tcgtaaaaca ctcctgcccc gcttcaggta
      121 tactgtgcac cttaattcca caaacatcag gcagaccatg acagacttaa tccaacgccc
      181 tcgtcgcctg cgcaaatctc ctgcgctgcg cgctatgttt gaagagacaa cacttagcct
      241 taacgacctg gtgttgccga tctttgttga agaagaaatt gacgactaca aagccgttga
      301 agccatgcca ggcgtgatgc gcattccaga gaaacatctg gcacgcgaaa ttgaacgcat
      361 cgccaacgcc ggtattcgtt ccgtgatgac tttcggcatc tctcaccata ccgatgaaac
      421 cggcagcgac gcctggcggg aagatggact ggtggcgcgt atgtcgcgca tctgcaagca
      481 gactgtgcca gaaatgatcg tcatgtcaga cacttgtttc tgtgaataca cttctcacgg
      541 tcactgcggt gtgctgtgcg agcatggcgt cgacaacgac gcgactctgg aaaatttagg
      601 caagcaagcc gtggttgcag ctgctgcagg cgcagacttc atcgcccctt ccgccgcgat
      661 ggacggccag gtacaggcga ttcgtcaggc gctggacgct gcaggcttta aagatacggc
      721 gattatgtcg tattcgacca agttcgcctc ttccttttat ggcccgttcc gtgaagctgc
      781 cggaagcgca ttaaaaggcg accgcaaaag ctatcagatg aacccaatga accgtcgcga
      841 ggcgattcgt gaatcactgc tggatgaagc ccagggcgca gactgcctga tggttaaacc
      901 tgctggagcg tacctcgaca tcgtgcgtga gctgcgtgaa cgtactgaat tgccgattgg
      961 cgcgtatcag gtgagcggtg agtacgcgat gattaagttc gccgcgctgg cgggtgctat
     1021 agatgaagag aaagtcgtgc tcgaaagctt aggttcgatt aagcgtgcgg gtgcggatct
     1081 gattttcagc tactttgcgc tggatttggc tgagaagaag attctgcgtt aatttttttc
     1141 ctctcagaaa gggggagtta acctgttgaa agtacggtga acgccccctc accctaaccc
     1201 tctccccaag ggggcgaggg aaccgtccgc actcgtatca ccccgcccga taaaacggtt
     1261 tatccccaag aatcgtcgcc cgatgcataa tccgccgctg tggcaggtaa tcggcattgg
     1321 catagtgctg agtcacgcgg ttatcccaaa tcgcaatatc attcggttgc cagcgccaac
     1381 gcacctgaaa ctccggttta gtgatatggg cgaataaaaa gcctaacaat gcttcgctct
     1441 ctttctcgct gacatcgaca attcgcgtag taaagccttc attcacaaac agcgcctgtt
     1501 taccgctcac cggatgcgtt cgcaccaccg gatgtagcaa cggaggattt ttcgcgaccg
     1561 cctcgcgcca gcgttgatgt tcctcctcgg ttttgcggta tttgtattcc gggaacgatt
     1621 tacggaaatc atgctccgca cgcagcccgc tcagcagctg gcggaaggga acagagagcg
     1681 cctcataggc cgcaataccg ctggtccaga gcgtatcacc gccggtcgaa ggtaattctt
     1741 tagctgccag aatcgcccct gcgggtggcg tttcaataaa tgtcacatcg gtatgccagt
     1801 tgtcgttatc tggcggatta tcgttatggg tatccagcac gatgatctcg tcaacccctt
     1861 cggcatgagg gtaaacaggg tgaatatgca gttcgccaaa acgctgggcc agcgcgcgtt
     1921 gctgctgcgg cgtaatggct tgatcgcgca gaaacaccac ctgatggcgc agcaccgcat
     1981 ggtaaagctg ttcaaactga ttatcgctta acgggcgcgt caggtcggca cccgaaattt
     2041 gtgcgccgat atacggcccc agcggggtaa tgctcagacg ttcactcatt gtacttctcc
     2101 atgccagggc gtcagacggc gctgtaacgc gcgcaaaccc agttctaaaa gaaaggcgat
     2161 aatcgcaatc accgcgattc ccgccagcac cacgtcagtc gcgagaaatt cacccgctga
     2221 ctgaaccata aatcctaaac cgcgcgtcgc ggcaatcagc tccgccgcta ccagcgtaga
     2281 ccagcccaca cccagaccaa tgcgtaatcc ggtgaggatt tccggcagcg cgccgggcaa
     2341 aatgacaaac cacagcacct gcgcacggct ggcacccagc gactgggcgg cacgaatgcg
     2401 aacctgctgc gcgcttttca cccccgccag cgccgacatc gccaccggtg caaaaatcgc
     2461 taaatagatc agtaagatct tcgaggtttc accaatacca aaccagatca ccatcagcgg
     2521 caaataagcc agcggcggca ccggacgata aagttcgatt accggatcca gaatgccgcg
     2581 caccgtcggg cttagcccca tggcgatccc gaccggaata ccgatcacca ccgccgcgag
     2641 tagcgccagc acaatgcgcg tcaggctggc tgccagatgc tgccacagcg tggcgtccat
     2701 aaatccctgc ggtccggcaa tggtgagcag ttttgccagt acttgttgcg gcggcggcaa
     2761 aaacagcggg ctaatcagtt gcagcgccgc caccgtccac catacagtga gtaaaaccgc
     2821 taacgtgcca atgcttaagg tcacctgacg cgagagcggc cagcgccatt tcagccgccg
     2881 cgaatgcagt ttttcattaa tgagcacact catgagaacg cctcccgttg ctcaaatacg
     2941 cggcttaaaa catattcgcg catggcgatg aattgtggat cggacttgat gctgcggctc
     3001 gactctcccg caacaaagcg gcgagcaaag ttgagcggca gccgctccag cacacggcca
     3061 gggccggatg aaagcagaac cagttcagtc gccataaaca ccgcttcttc tatatcgtgg
     3121 gtaatcaaca gcacctgctt gcccgtctcc tgccagagtt tcagcagcag agtttgcatc
     3181 tggtcgcggg tgaaggcgtc cagcgcacca aacggttcgt cgagtaataa cagctgggga
     3241 ttcgccgcca gcgcgcgggc aatccccacc cgctgacgtt gaccaccgga aagctgccag
     3301 atatagcgtt tttctgcgcc ttccagcccc acttttttca gcatctggtg cgcgatttcc
     3361 agtcgctgca ttttctctat acctgccaat tgcaggccga acgccacgtt gtcctgtaca
     3421 ttgcgccacg gtagtagccc ttcattctga aaaaccaccc cgcgctctgc tcccggtccc
     3481 tcaatacgct tacccgccag ttgaatgctg ccatgctgat aaggcacaaa accggcaatc
     3541 agattcagca acgtggtttt accgcagccg gacggcccca gcaccaccag tagctcgccg
     3601 ctttccagcg tcaggttgat atcttccagt gccggtttgc cgccataatc ggcgtaaaga
     3661 tgagagattt gcagcatctg cgcctccttt tattgcacga agcgcgaggt aacgtactgg
     3721 ctgtaatcat tcgctacagc cgggaccttg ccctgctctt tcaaaaactg cgcggtgtcg
     3781 atgatcgctt tgttcaccgg tccggtcagt tctaccgttt gttgctgcgg cgtcagatag
     3841 gtattcccct tcaccagccc cggaacgtca ccttccggca cgccgcttaa acgcgccagt
     3901 ttgctgatgt tttccggctg tttcagccac gcgtctgggt tagcaatgta cggttgctga
     3961 gcatcgatgg cgcttttagc gaacgctttc acgacctcag gatgtttctc ggcaaaatct
     4021 ttgcgcacca cccagacgtc cagcgttggc gcaccccact gcccgacctg ttcagaatcg
     4081 gtcaacacct tgccgtcttt ttccagggcg ttaaccgccg gtgcccagac ataagcacca
     4141 tcaatatcac cccgctgcca tgcggcgata atcgcgggcg gctgcaggtt cacaatctcc
     4201 acttgccctg gtttaatccc ccagtgtttc agcgccgcca gcaggctgta gtgggtggtg
     4261 gagataaacg gtacggcaat gcgtttgcca atcaaatctt cgggtttgct gatagttttc
     4321 tttaccacca gcgcttcgga gttacccagt tttgacgcca gcaagaagac ttcaatcggc
     4381 acctgttggc tggctgcaac cgctaatggg ctggaaccga ggttgccgat ttgcacgtcg
     4441 ccagaagcca gcgcccgcac gatgctggct ccgctgtcaa acttacgcca gtccacggtt
     4501 gctccgcttt ctttagcaaa ggtgttgtcg gcctgagcca ctttcgccgg ttcggctgag
     4561 gtttgatacg ccacggtgac gttcaccgcc tgtgcctgaa aagcgatgaa tgccagtgcg
     4621 gcaagaagtg tgttacgcga tgaaattgcc atgattgtct gctcccctgt cttgttatgt
     4681 gagcagtatt caggaataaa aacattcatt aaaagaatta gtcgttatcg tacagatgat
     4741 tttattctta gcaaaaaaac ggcaaaggaa agattaaata tgcaattact ccgttctaac
     4801 aattgggaaa attactacac ttcat
//
LOCUS       DAZKIV010000234         4822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZKIV010000234 DAZKIV010000000
VERSION     DAZKIV010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.236"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            167..1567
                     /gene="gabP"
                     /locus_tag="ACHOT6_004530"
     CDS             167..1567
                     /gene="gabP"
                     /locus_tag="ACHOT6_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFP9949618.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            1588..2250
                     /gene="csiR"
                     /locus_tag="ACHOT6_004531"
     CDS             1588..2250
                     /gene="csiR"
                     /locus_tag="ACHOT6_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFP9949619.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(2251..2700)
                     /gene="kbp"
                     /locus_tag="ACHOT6_004532"
     CDS             complement(2251..2700)
                     /gene="kbp"
                     /locus_tag="ACHOT6_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFP9949620.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(2784..2942)
                     /locus_tag="ACHOT6_004533"
     CDS             complement(2784..2942)
                     /locus_tag="ACHOT6_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFP9949621.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            3125..3424
                     /locus_tag="ACHOT6_004534"
     CDS             3125..3424
                     /locus_tag="ACHOT6_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFP9949622.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            3434..3958
                     /gene="ygaP"
                     /locus_tag="ACHOT6_004535"
     CDS             3434..3958
                     /gene="ygaP"
                     /locus_tag="ACHOT6_004535"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFP9949623.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLCEHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(4005..4409)
                     /gene="stpA"
                     /locus_tag="ACHOT6_004536"
     CDS             complement(4005..4409)
                     /gene="stpA"
                     /locus_tag="ACHOT6_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFP9949624.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         1157 a         1163 c         1186 g         1316 t
ORIGIN      
        1 aatcacgcgg ctacaccctc accccggccc tctccctaag ggagagggag aaaaccctcc
       61 cgctttcgtt tttaagattt tgttgaccgt ctcaattaaa cgcgtgaccc gacgcgggaa
      121 atgtcggggc gctctcccaa gtgacacact ttcgagagga ttcaggatgg ggcaatcatc
      181 gcaaccacat gagttaggcg gcgggctgaa gtcacgccac gtcaccatgt tgtctattgc
      241 cggtgttatc ggcgcaagtc tgtttgtcgg ttccagcgtc gccatcgccg aagcgggccc
      301 ggcggtatta ctggcctatc tgttcgccgg attactggtg gttatgatta tgcggatgtt
      361 ggcggaaatg gcagttgcca cgcccgatac cggttcgttt tccacctatg ccgataaagc
      421 cattggccgc tgggcgggtt ataccatcgg ctggctgtac tggtggtttt gggtactggt
      481 tatcccgctg gaagccaaca tcgccgctat gatcctgcac tcgtgggttc caggcattcc
      541 catctggtta ttttccctcg tcattaccct cgccttaact ggcagtaatt tattaagcgt
      601 taaaaactac ggcgaatttg agttctggct ggcgctgtgc aaagtcatcg ctatcctggc
      661 ctttattttc cttggtgcag tcgcaattag cggtttttac ccttatgccg aagtgagcgg
      721 gatctcaaga ttgtgggata gcggcggctt tatgcccaac ggtttcggtg cggtattaag
      781 cgcgatgttg atcaccatgt tctcgtttat gggcgcagaa attgtcacca ttgccgccgc
      841 ggaatccgac acgccggaaa aacatattgt ccgcgccact aactcggtta tctggcgtat
      901 ttctatcttc tatttgtgct ctatttttgt cgtcgtggca ttaattccgt ggaatatgcc
      961 cggactaaaa gccgtcggtt cttatcgctc ggtactggaa ttgctcaata ttccccatgc
     1021 gaaattaatc atggactgtg tgatattact ttccgtaacc agctgtctga actcggcgct
     1081 gtataccgcg tcaaggatgc tctactcctt aagccgtcgc ggtgatgcgc ccgcggtaat
     1141 gggcaaaatc aaccgcagta aaaccccgta tgtggcggtg ttactctcca ccggagcggc
     1201 atttttaacg gtggtggtga actattacgc acctgcgaaa gtgtttaaat tcctgataga
     1261 cagctccggt gctatcgccc tgctggttta tttagtcatc gccgtttcac agttgcggat
     1321 gcgtaaaatt ctgcgagcag aaggaagcga aattcgcttg cgcatgtggc tttacccgtg
     1381 gctcacctgg ctggtaatag gctttattac ctttgtgttg gtggtgatgt tattccgccc
     1441 ggcgcaacag ttagaagtga tctctaccgg cttattagcg atagggatta tctgtaccgt
     1501 gccgattatg gcgcgctgga aaaagctggt attgtggcaa aaaacacccg ttcataatac
     1561 gcgctgataa tgatcaggag tcacaccatg accattacgt ctctggatgg ctatcgctgg
     1621 ctgaagaacg atattattcg cggtaatttt caaccggatg aaaaattacg aatgagtttg
     1681 ctgacatcgc gttatgcact tggcgttggg ccgttacggg aagctctttc gcaactggtg
     1741 gcggaacggc tggtcacggt ggtgaatcaa aaagggtatc gggtggcgtc tatgtcagag
     1801 caggagctgc tcgatatttt cgacgcccgc gccaatatgg aagcgatgct ggtcagtctg
     1861 gcgattgccc gcggtggcga tgaatgggag gcagacgttc tcgcaaaagc gcatctgctg
     1921 agtaagctgg aggcctgtga cgccagcgag aaaatgcttg atgagtggga tctgcgtcat
     1981 caggcgtttc atacagcaat tgtggcgggc tgtggttctc actatttgct gcaaatgcgt
     2041 gaacggttgt ttgatctggc ggcgcgctat cgatttatct ggctgcggcg aacggtgctt
     2101 tcggtagaaa tgctggagga taaacacgat cagcaccaga ccctgactgc ggcggtactg
     2161 gcgcgagata ccgcgcgcgc cagtgagtta atgcgccagc atttactgac gccaattccc
     2221 attatccagc aggcgatggc tggcaattaa ctactcttcc ggaatacgca acacttgccc
     2281 cggataaatt ttatccgggc tttttagcat cggtttattc gcttcgaaga ttttattgta
     2341 cagattagcg ttaccgtaga cctgtttgga aatggcactc agagtgtcgc cagacttaac
     2401 ggtataaaac tggctggcag tggctggtgt cgccgttttc acctgatcat caacactggc
     2461 aataccggaa atattcccca ccgcaacaag gattttctcc ttcgcctcct gactcaggcc
     2521 gtcaccagtg accgtcgctt tgccgtcggc aatttgaatg ttcactttat cggcatcagg
     2581 tataccggtt ttgctcagat gctcctgcac cttcttcgcc tgatcgtctt tatcgtgctg
     2641 acctgtaacc gcgtcccaga gtttttctcc ggcatctttc acaaaattga acagacccat
     2701 agctacctca ttcgttaacg gaaacactca gaaagtgtag cagaggaacg cggcgcgtta
     2761 acgccgcggg ggtgagcaca ggtttaatcg cgggtttgca cccagaacgc gtgaatcaaa
     2821 ccggggatat acccaagcag tgtcagcaga atattaataa tgaacgccca accgaaccct
     2881 ttaccgagca gcacgccgag aggcggcaga atgatggtga tgacgattct ccagaaaccc
     2941 atatgtactc cctataagaa aattactcat tgtttaaaaa gagattttat ctcttaagcg
     3001 tagtaatttt agcggagcct gccagtttca gcttacgctt ttactctcat ttacgctgct
     3061 gatgctggta tatgttgcat ttttagttta gagtttactt aatttagaaa atacttaaat
     3121 aaatatgact gaactcgcgc aattacaggc cagtgccgaa caggcagcgg ccttattgaa
     3181 agcaatgagc catcctaaac gattgctgat tctgtgcatg cttagcggtt cccccggcac
     3241 cagcgcggga gagctgacgc gcattaccgg actgagtgcc tctgcgacat cacagcatct
     3301 cgctcgtatg cgggacgaag ggcttatcga cagccaacgg gatgcccaac gcattctata
     3361 ttccattaaa aatgaggcgg taaatgccat tatcgccacc ctgaaaaatg tctattgtcc
     3421 gtaaggagtc actatggctt tgacaaccat ttcgccgcat gatgcacaag aattaatcgc
     3481 acgcggcgca aagttaatcg atattcgtga tgctgatgaa tatctttgtg aacatattcc
     3541 tgaagcagat ctggctccat tatccgtgct ggaacagtca ggtcttccac ctaaattacg
     3601 tcgcgagcaa attatattcc actgccaggc aggtaaacgc accagtaata acgccgataa
     3661 attagcagcg attgccgctc ccgcagaaat atttttactc gaagatggga ttgatggctg
     3721 gaaaagagct ggattgccag tagcggtaaa taaatctcaa cccttgccgt taatgcgcca
     3781 ggtgcagatc gctgcgggtg gtttaatatt aatcggcgtt gtactgggtt ataccgtaaa
     3841 tagcggtttc ttcttattaa gtggctttgt tggtgccggg ttactgtttg caggaatcag
     3901 cggtttttgt ggaatggcaa ggttgttaga taagatgccg tggaaccaac gagcttgaga
     3961 agcgacgccg gacgcgccct agcagcgaca tccggcctca gtaattagat caggaaatcg
     4021 tcgagagatt taccttctgc cagcgcctga gcaattggct tcggtgtacg gccctgaccg
     4081 gtccaagttt tagtttcacc gttaacatcg gtgaatttat atttcgccgg acgcggctgg
     4141 cgttttttac cagcgcgtgg tgcagcagca gagctattac ccaataactc ttccgggtta
     4201 attccgtcag ccttcatcag ctccagccag gtgctaattt tttcctgacg ctcagccagt
     4261 tcacgctgct gctgttcttc ttcttcacgt ctttctttag tgacaaccct gaatttttcg
     4321 agcatttctt caagaacgtc aatggagaat tcgcgagcca tcgcacggag ggtgcgaatg
     4381 ttatttaaac tttgtaacat tacggacata ataaagaaaa ccttttaacg ccaaaacaaa
     4441 aaagtatttc gtcgcgtagt gtaagcgatc acttattata tttccacaaa atttaatcta
     4501 tttacagtcc tgcgcgagat tatttcagct gttattttct gaaactctca gaataatttc
     4561 aagattatat attagccatt ttgatgagta atttagatta ttggtgaaca gagtggcgct
     4621 ctctgctcgc taattccgca agcctgacaa aaccgaagtg aataaaaatg caatataaca
     4681 gaataaaaga aaacacccct cactggtgct ttctgctgtt aatgtacatt ttctggcata
     4741 aaaaatgctg taaatgacac ggtcacgaca tacaccgaat tctctacgct tatagcgtta
     4801 cctcaccccc aaactttcat ta
//
LOCUS       DAZKIV010000235         4726 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.237, whole genome shotgun sequence.
ACCESSION   DAZKIV010000235 DAZKIV010000000
VERSION     DAZKIV010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.237"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..327)
                     /gene="gadE"
                     /locus_tag="ACHOT6_004537"
     CDS             complement(<1..327)
                     /gene="gadE"
                     /locus_tag="ACHOT6_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFP9949625.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPH"
     gene            complement(1126..1698)
                     /gene="hdeD"
                     /locus_tag="ACHOT6_004538"
     CDS             complement(1126..1698)
                     /gene="hdeD"
                     /locus_tag="ACHOT6_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFP9949626.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            1953..2285
                     /gene="hdeA"
                     /locus_tag="ACHOT6_004539"
     CDS             1953..2285
                     /gene="hdeA"
                     /locus_tag="ACHOT6_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFP9949627.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            2401..2727
                     /gene="hdeB"
                     /locus_tag="ACHOT6_004540"
     CDS             2401..2727
                     /gene="hdeB"
                     /locus_tag="ACHOT6_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFP9949628.1"
                     /translation="MNISSLRKAFIFMGAVAALLLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            2791..3438
                     /locus_tag="ACHOT6_004541"
     CDS             2791..3438
                     /locus_tag="ACHOT6_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFP9949629.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(3480..4010)
                     /locus_tag="ACHOT6_004542"
     CDS             complement(3480..4010)
                     /locus_tag="ACHOT6_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFP9949630.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(4166..>4726)
                     /gene="slp"
                     /locus_tag="ACHOT6_004543"
     CDS             complement(4166..>4726)
                     /gene="slp"
                     /locus_tag="ACHOT6_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFP9949631.1"
                     /translation="MTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQPG
                     LYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFLDP
                     VNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYGYG
                     AFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT         1451 a          960 c          973 g         1342 t
ORIGIN      
        1 atggggcaag tgtttaccat aagtaatctc aagaagattt ttgatacttt ctttgcggct
       61 aacaaaataa ataggcgctt tagcttttag tttactgatg tgataaggtg ccaaaacaat
      121 aattctctcg gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt
      181 gtgataggta tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag
      241 ggaattgatt tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa
      301 agaatctttc gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat
      361 gtcatccttt tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta
      421 taagaacttt gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt
      481 atccatcacg cactccatat attgatccta agtaagattc ctggttgtta tcaatttgta
      541 aatatcaaac aaaaagttaa cgtttgcgga ttactgccca agaataagtt atcaataacc
      601 agtttgtgat ctctgaagaa tattactaaa gttaaaatgt aatccgattt aaatatcgag
      661 tctccttgtt tcgacttaag ctggcaattg gattgccagc tttcttttaa cgcattgaaa
      721 ttacctgagt aaataacgca ttcatgtctt caggttttta tatagcgtct ggagacacgg
      781 atatttatgc aatgtaatca acgccagttg caaaaaaatg aacccggatt acttatttaa
      841 ccgcccatag catcaacgct tacggaactc tttgttcact ttcccacagc acttttaaaa
      901 cattaaacct gactacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca
      961 tggagtgaaa aaaattagat gaaattcagt aggttgaaat gatcactagc aggtaattat
     1021 ttcaatgata gtgcgcaatt gatctacaac actgcgtagc ggagagagta ttaatcggat
     1081 catagtcaca tcaagtgact atgatccggg tgacaaccgg gataattatt gctgcttaac
     1141 gaacaaactg gcgaagctga acaggctggc ggcgctaaat atcagttcaa ttcccaccag
     1201 tgtggaaacc agcgttacag acaccatcgg cgttgcacca aggaatatcc aggcaatgac
     1261 gatatccagc acaccaataa cgagctgtag ccagctgcct ttcattgaac gctgacgata
     1321 ccaactcatc aggcgaataa cccctgcaac acagaacaaa ccggcaataa atgccgcaat
     1381 ggcaaaaatg cccagctccg gtgcgcggat gaagaaatag ccgatcaata aataggcgac
     1441 tgcgacgagg aaaccggata ataccggcca gaaattatga ctgcggttgc tgaataaccc
     1501 gacaataagc gcaatacccg agcagattaa taatgcaccc actactgtgc ttaaaatatc
     1561 gccagagacg aacgggaaac tgatacacag caacccgacg ataaacagca gcacggcaat
     1621 aaactggatt gctctgcgat gttttttaag catctccaga tcaaacttca aaattgttgc
     1681 cttatctata tataacatag aaccacccta taaaattaag aagaaaatcc cctgctatca
     1741 atctatgcca aaaacgcgtc taagaatgca gtcgatttaa taaaaatttc ctaattgcag
     1801 tatctgatgc atctgtaact cattgtattg aaataaaaat atctgatttt gatattttcc
     1861 atcaacatga catatacaga aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac
     1921 ggctctttca ctttatagtt gaggatatta cgatgaaaaa agtattaggc gttattcttg
     1981 gtggtctgct tcttctgcca gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata
     2041 acaaaaaacc ggtcaactcc tggacctgtg aagatttcct ggctgtggac gaatccttcc
     2101 agccaactgc agttggtttt gctgaagcgc tgaacaacaa agataaacca gaagatgcgg
     2161 ttttagatgt tcagggtatt gcaaccgtaa ccccagctat cgttcaggct tgtactcagg
     2221 ataaacaagc caactttaaa gataaagtta aaggcgaatg ggacaaaatt aagaaagata
     2281 tgtaattccg ggaatgcgtt acatcgtact tccttgcata ttgaacaggc cggaatatct
     2341 tctttaaaag cagctattcc tcctgttcat atataatctc tatattgaat gggttacaaa
     2401 atgaatattt catctctccg taaagcgttt atttttatgg gcgctgtagc ggctttgtta
     2461 ctggtgaacg cacaatctgc gctggcagcc aatgaatccg ctaaagatat gacctgccag
     2521 gaatttattg atctgaatcc aaaagcaatg accccggttg catggtggat gctgcatgaa
     2581 gaaacagtat ataaaggtgg cgataccgtt actttaaatg aaaccgatct cactcaaatt
     2641 cctaaagtga tcgaatactg taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat
     2701 caagcatcta atgacttgcc gaattaatga ggtacaagta aaaaggagta gcaagttgag
     2761 ccatcttgct gctccttttt gcatttttat atgacagcag aatttattat acgtcttata
     2821 ctcgcggcaa ttgcctgtgg cgctattggc atggaaaggc aaatgcgcgg caaaggagca
     2881 gggttacgca cacatgtatt aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa
     2941 tatggttttg ctgacgtgct gtctttagat cacgtcggac tcgaccccag ccgtattgct
     3001 gctcaggtgg tgacgggcgt cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa
     3061 aatattgtag gtctgacgac ggcagcggat atctgggtga ccgccgccat aggtatggtt
     3121 attggcagcg gtatgtacga actgggtatt tatggttcag tgatgacctt gttggtgctg
     3181 gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt acagctgaca
     3241 ttagtgaacg gtaatacggt atcaatgctc gactggttta agcagcaaaa aataaaaacg
     3301 gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga tatccagctt
     3361 catgccacaa cgtcgataga agacctgttg cgattattaa aaggtatggc aggggtgaaa
     3421 ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt
     3481 cacaccagat aatcaatatg ctgatggcgt accagctcat tgatccgctt aagattgagg
     3541 atcatcatga tattgtgttt atggcagtag aacgttttta gcgatatttt cagtttctcc
     3601 agaatatctt ccgttgtcat ttgattcgca atgcagctca gaacctgatg ttgcttgcgc
     3661 gaaagtgaca cagccgcttc aggcacagag cagtaactgc cattgatgag tgcgacaaaa
     3721 tcaatgatag tgatagtccg cggaacaaag gtcaccggca ctttccccag gcagcgttta
     3781 atattaaagg gggagaaaat aatcacatgg acaggtttta atcgttcaat ttgagtgaga
     3841 aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc attctgatgc
     3901 aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt actcagaatg
     3961 tttttcatcg cggtgaagaa catcgtatcc ctggtaatta taagaaacat aaccgactcc
     4021 tggtgcggac attaacttta taatcagttt gccaaaggta attaacagga cagggcttat
     4081 aataataata gttagcttac tgtatattaa ttttatgtaa tacatgatgc agatgctgag
     4141 aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc tgactcaccg
     4201 cattggtgta gtaaggcgca ccccagcccg gttccggcca gaatgcacca tagccgtaat
     4261 cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg atgccctgca
     4321 tattcacttc caggaagtta tacgggactt tattgataaa gcctggttgt tcaccctgaa
     4381 tggtaccgag gatggtaaca aagtgattac gatagttcac tggatcaagg aagccgcttt
     4441 gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggcttcgca tagctatcca
     4501 acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg ttgataacct
     4561 tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga acagcaacaa
     4621 aacttttttg aatatcaggt tggttattgc ctttgatatt ttgcggaatt gaactacatg
     4681 cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt tgtcat
//
LOCUS       DAZKIV010000236         4578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZKIV010000236 DAZKIV010000000
VERSION     DAZKIV010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.238"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(56..319)
                     /locus_tag="ACHOT6_004544"
     CDS             complement(56..319)
                     /locus_tag="ACHOT6_004544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949632.1"
                     /translation="MFDGNNKLHENVKIFIQRVDLGNAQTPREIVRSLRHYEQKAMDY
                     FENIPKINGSQNGGRAAAVSKVEEFAKVAKEKLTSAGEWVILK"
     gene            complement(353..>4578)
                     /locus_tag="ACHOT6_004545"
     CDS             complement(353..>4578)
                     /locus_tag="ACHOT6_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9949633.1"
                     /translation="LMYASYKASDYVEEKQNQLESWINSFCDTDGAINTGSENVNING
                     KPAARAAVTLPPPPPPGAIPEVPQGEPSWGDIATDLLESAAEKAVPLAKAWGNAVITL
                     TDSNAGFMDRLCAGTSLLFPAGPVLMEFATMVGGRGEIKKEVDFPEAGEDTALCDKEN
                     KPPRIAQGSSNVFINNRPAARQGDKLECSAAIVGGSPDVFIGGEQVTYLDIQPEFPPW
                     QRMILGGITIASYLLPPAGLLGKLKNLARLGKLGNLLGKSGKLLGAKLGALLGKTGKS
                     LKSIANKVIRWVTDPVDPVTGAYCDERTDFTLGQTLPLSFTRFHSSVLPLHGLTGVGW
                     SDSWSEYAWVREQGNRVDIISQGATLNFAFDGDSDTAVNPYHAQYILRRRDDYLELFD
                     RDALSSRFFYDAFPGMRLRHPVTDDTSDDRLAHSPDDRMYMLGGMSDTASNRITFERD
                     SQYRITGVSHTDGIRLKLMYHASGYLKAIHRTDNGIQTLATYEQDARGRLTEADARLD
                     YHLFYEYDAADRIIRWSDNDQTWSRFTYDEQGRCVNVTGAEGYYNATLDYGDGCTTVT
                     DGKGIHRYYYDPDGNILREEAPDGSTTTYEWDEFHHLLARHSPAGRVEKFEYNAAHGQ
                     LSRYTAADGAEWQYRYDERGLLSNITDPAGQTWTQQCDERGLPVSLVSPQGEETRLAY
                     TPQGLLSGIFRQDERRLGIEYDHHNRPETLTDVMGREHHTEYSGHDLPVKMRGPGGQS
                     VRLRWQQHHKLSGLERAGTGAEGFRYDRHGNLLAWTDGNGVVWTMEYGPFDLPVARTD
                     GEGHRWQYRYDKDTLQLTEVINPQGESYRYILDNCGRVTEERDWGGVVWRYRYDADGL
                     CTARVNGLEETILYSRDAAGRLAEVISPEGKTQYAYDKSGRLTGIFSPDGTSQRTGYD
                     ERGRVNVTTQGRRAIEYHYPDEHTVIRCILPPEDERDRHPGESLLKTTYRYNAAGELT
                     EIILPGDETLTFSRDEAGREVLRHSNRGFACEQGWNAAGQPVSQRAGFFPEEATWSGL
                     VPSLVREYRYDSAGNVSAVTSREDYGRETRREYRLDRNGQVTAVTASGTGLGYGEGDE
                     SYGYDSCGYLKAQSAGRHRISEETDQYAGGHRLKQAGNTQYDYDAAGRMVSRTRHRDG
                     YRPETERFRWDSRDQLTGYCSAQGEQWEYRHDASGRRTEKRCDRKKIRFTYLWDGDSI
                     AEIREYRDDKLYSVRHLVFNGFELISQQFSRVRQAHPSVAPQWVTRTNYAVSDLTGRP
                     LMLFNSEGKTVWRPGQTSLWGLALSLPADTGYPDPRGELDPEAAPGLLYAGQWQDAES
                     GLCYNRFRYYEPETGMYLVSDPLGLLGGEQTYRYVPNPLGWIDPYGLLCRRGQLKALG
                     IPDSPGVYHIILDNKVYTGSGKSILNRLA"
BASE COUNT          829 a         1429 c         1227 g         1093 t
ORIGIN      
        1 gcagaaccca acgttctgaa ctacgtagag taaaagagtt cattcaaatg ctcctttatt
       61 ttaaaataac ccactcacct gcgctagtta atttttcttt tgcgacctta gcaaactctt
      121 caaccttaga aaccgccgca gccctccccc cattttgaga accattaatt ttcggtatgt
      181 tttcgaaata atccattgcc ttttgttcat aatgtcgaag agagcgaaca atctctctag
      241 gagtttgagc atttcccaag tcaactcttt ggataaatat tttcacattt tcatgcagtt
      301 tattattacc atcaaacaat aattctgtag cctttttgtg ccctcccaat cctcaagcta
      361 atcgattcag aatacttttt cctgaaccag tgtacacttt attatccaat ataatatggt
      421 aaacacctgg actatccgga attcctaacg ctttaagttg tcctcttctg cacaacagac
      481 cgtatggatc aatccacccc agtggattcg gcacataccg gtaagtctgc tcacccccca
      541 gcaaccccag cgggtcactc accaggtaca tcccggtttc cggctcgtaa taccggaacc
      601 ggttatagca cagcccggat tccgcatcct gccactgtcc tgcatacagc aggccggggg
      661 cggcttccgg gtccagttcc ccgcgcgggt ccgggtagcc ggtgtctgcg ggcagactga
      721 gtgccagccc ccacaggctg gtctgccccg gtcgccagac ggttttacct tcactgttaa
      781 agagcatcag cggacggccc gtcaggtcgc tcaccgcata attcgttcgc gtcacccact
      841 gcggggccac cgacggatgc gcctgccgta cccggctgaa ctgctggctt atcagctcaa
      901 agccgttaaa caccaggtgc cgtacgctgt acagtttatc atcgcggtat tcccggattt
      961 ccgcaatact gtcgccgtcc cacagatacg tgaagcggat tttcttcctg tcgcagcgtt
     1021 tctctgttcg tctgccgctg gcgtcgtggc ggtattccca ctgctcaccc tgtgcgctgc
     1081 aatacccggt cagctggtca cggctgtccc accggaaccg ctctgtttcc gggcgatagc
     1141 cgtcacggtg tctggtgcgg ctgaccatcc ggcctgcggc gtcatagtca tactgcgtgt
     1201 ttccggcctg tttcagccgg tggcctccgg catactggtc agtctcttca cttatccggt
     1261 gcctgcccgc agactgcgcc ttcaggtagc cacaactgtc atagccatag gactcatcgc
     1321 cttcgccata gcccagcccg gtgcctgagg ctgtcaccgc cgtgacctga ccgttccggt
     1381 ccagccggta ctcccgccgt gtttcccgtc cgtagtcttc ccggctggtg acagccgaca
     1441 cattacccgc gctgtcgtaa cggtactccc gtaccagtga cggaaccagc ccgctccatg
     1501 tggcttcttc cggaaaaaat ccggcgcgct gactgacagg ctgaccggct gcattccagc
     1561 cctgttcaca ggcaaaaccc cggttactgt gccggagcac ttcacgtccc gcctcatcac
     1621 ggctgaacgt cagcgtctca tcccccggca ggataatctc cgtcagttct ccggcggcgt
     1681 tgtagcggta cgtggttttc agcagggatt caccggggtg tctgtcgcgt tcatcttccg
     1741 gtggcaggat acagcggatg acggtgtgtt cgtcggggta gtggtattca atggcccgtc
     1801 ggccctgagt ggtgacattc acccgcccgc gttcgtcgta gccggtgcgc tgtgatgtgc
     1861 cgtccgggct gaagataccc gtcagcctgc cggatttgtc ataggcatac tgcgttttgc
     1921 cttccggcga gatgacttct gccaggcggc ctgcggcgtc ccggctgtag aggatggttt
     1981 cctccaggcc gttgaccctg gcggtacaca ggccatcggc gtcatagcgg taacgccaga
     2041 ccacgccgcc ccagtcacgc tcttccgtca cccgtccaca gttgtccaga atataacggt
     2101 atgactcgcc ctgcggattg atgacttctg tcagttgcag cgtgtcttta tcgtagcggt
     2161 actgccagcg gtggccttca ccgtccgttc gcgccaccgg caaatcgaac gggccgtatt
     2221 ccatcgtcca gacaacgccg ttgccgtccg tccaggccag caggttgccg tggcggtcat
     2281 agcgaaatcc ttccgcgccg gttccagcac gttcaaggcc gctcagttta tggtgctgct
     2341 gccaccgtaa ccgtactgac tgaccgccgg ggccgcgcat cttcaccggc aggtcgtgac
     2401 cgctgtattc ggtatggtgt tcacggccca tcacgtcggt gagtgtttcc ggccggttgt
     2461 ggtggtcgta ctctatgccc agacgccgtt catcctggcg gaatatcccc gacagcagcc
     2521 cctgcggcgt gtacgccagc cgggtctctt cgccctgtgg cgacaccaga cttaccggca
     2581 ggccgcgttc atcacactgc tgtgtccacg tctgcccggc tgggtcggtg atgttgctga
     2641 gcaggccgcg ctcatcatag cggtactgcc actccgcgcc gtctgccgcc gtgtaacggc
     2701 ttaactgacc gtgtgcggcg ttgtattcaa acttctccac ccgcccggca ggggagtggc
     2761 gggccagcag gtgatggaat tcatcccatt catacgtggt ggtgctgccg tccggcgctt
     2821 cttcccgcag aatattgccg tcaggatcat agtaataacg gtgaatgccc ttgccgtccg
     2881 tcacggtggt gcagccgtca ccatagtcca gcgtggcgtt gtaatagccc tccgccccgg
     2941 tgacattcac gcaccggccc tgttcatcgt aggtgaaacg gctccacgtc tggtcgttat
     3001 cggaccagcg gatgatccgg tccgcagcgt cgtactcata aaacaggtgg tagtccagcc
     3061 gcgcatccgc ttctgtcagc cgcccccgcg catcctgttc gtaggtcgcc agcgtctgta
     3121 tgccgttatc cgtgcggtga atggctttca ggtagccgct ggcgtggtac atcagtttaa
     3181 gccggatccc gtcggtgtga ctgacacccg tgatgcggta ctggctgtcg cgctcaaacg
     3241 tgatgcggtt gctggcggtg tcgctcatcc cgcccagcat atacatccgg tcgtcggggc
     3301 tgtgtgccag gcggtcatcg ctggtatcgt cagtcaccgg gtggcgcaga cgcattcccg
     3361 gaaaggcgtc ataaaagaag cggctgctca gtgcatccct gtcgaacagc tccaggtaat
     3421 cgtcgcggcg gcgcagaatg tactgggcgt gatacgggtt aaccgccgta tcactgtcac
     3481 cgtcgaaggc gaagttcagc gtggctccct ggctgataat atctacccga tttccctgtt
     3541 cacgcaccca ggcgtattcg ctccaggagt cgctccagcc cacgccggtc aggccatgca
     3601 gcggcagtac agaactgtgg aaacgggtga aggagagggg gagtgtctgg cccagggtga
     3661 agtcggtacg ttcgtcgcag tacgcgccgg ttaccggatc gacaggatct gttacccatc
     3721 tgatgacttt attggcaata ctttttaacg acttacctgt tttccccagc aacgcgccga
     3781 gctttgcccc cagcagcttc ccgcttttcc ccagcaggtt ccccagtttg ccgagtctcg
     3841 ccagattctt cagttttccc agcagtcccg ctggcggcag aagatagctg gctatcgtta
     3901 ttccccccag gatcattctc tgccacggcg ggaactccgg ctggatatcc agataggtca
     3961 cctgctcacc gccaataaag acgtccggcg aacctcccac gattgctgcg ctgcactcca
     4021 gtttgtcgcc ctggcgtgcg gcaggccgat tgttgatgaa gacgttgctg ctgccctggg
     4081 ctatcctcgg tggtttgttc tccttgtcgc agagcgccgt gtcctcaccg gcttccggga
     4141 aatccacctc ttttttgatt tcgccacgcc cgcccaccat ggtggcaaac tccattaata
     4201 ccggaccggc gggaaacaga agcgatgtgc ctgcacataa gcgatccata aaaccggcat
     4261 tgctgtccgt cagggtgata acagcgttcc cccaggcctt cgccagtggt actgcttttt
     4321 ccgctgccga ttcaagcagg tcagtggcaa tatcacccca tgagggttcc ccctgtggga
     4381 cttcaggtat tgctccaggt gggggaggag ggggaagggt gacggcggcc ctggcagcgg
     4441 gctttccgtt aatgtttaca ttttcagaac cggtattgat ggcgccgtcc gtgtcacaaa
     4501 agctgtttat ccatgattca agctggttct gtttctcctc cacataatca ctcgccttat
     4561 agctggcata catcagcg
//
LOCUS       DAZKIV010000237         4570 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZKIV010000237 DAZKIV010000000
VERSION     DAZKIV010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.239"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..170)
                     /locus_tag="ACHOT6_004546"
     CDS             complement(<1..170)
                     /locus_tag="ACHOT6_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WlaTC/HtrL family glycosyltransferase"
                     /protein_id="HFP9949634.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSP"
     gene            474..1406
                     /gene="rfaD"
                     /locus_tag="ACHOT6_004547"
     CDS             474..1406
                     /gene="rfaD"
                     /locus_tag="ACHOT6_004547"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFP9949635.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1416..2462
                     /gene="rfaF"
                     /locus_tag="ACHOT6_004548"
     CDS             1416..2462
                     /gene="rfaF"
                     /locus_tag="ACHOT6_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFP9949636.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEDYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2466..3458
                     /gene="rfaC"
                     /locus_tag="ACHOT6_004549"
     CDS             2466..3458
                     /gene="rfaC"
                     /locus_tag="ACHOT6_004549"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFP9949637.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3455..>4570
                     /gene="rfaL"
                     /locus_tag="ACHOT6_004550"
     CDS             3455..>4570
                     /gene="rfaL"
                     /locus_tag="ACHOT6_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFP9949638.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFI"
BASE COUNT         1241 a         1025 c         1084 g         1220 t
ORIGIN      
        1 tcgggagaag tgaaaataat catctcattt tcaagtgcgg ccagacgctc aaagtaacta
       61 aaataaacat caactgaacg agctagtttt tcacggaacc ctttattagc agtccaatcc
      121 cctctaccaa tgtcaaaata tgcagtgata attgtggtgg atgatttcat tactaataag
      181 accattaata taaaaaaaga attatatcat aaatattaac agaatgtata ctgtatatta
      241 tccattcaaa tgcttccgtt atttatcata catcggaata ttgatactaa agcactattc
      301 acatgcaaaa ccaacatccg ccatgaagga ctagctaaaa cccaaactag tttgttgcaa
      361 ttagcatcct tgcacctcta tgtaaagggc tgaagggatt cggatgtgat ggtatgatta
      421 cagacattcg tgtctgagat tgtctctgac tccataattc gaaggttaca gttatgatca
      481 tcgttaccgg cggcgcgggc tttatcggca gcaacatcgt taaagccctg aatgataaag
      541 gcatcaccga tattctggtg gtggacaacc tgaaagacgg caccaagttt gtgaacctgg
      601 tggatctgaa tatcgcagac tatatggata aggaagactt cctgatccag attatggctg
      661 gcgaagagtt cggcgatgtc gaagcgattt tccacgaagg cgcgtgctct tccaccaccg
      721 agtgggacgg caagtatatg atggataaca actatcaata ctccaaggag ctgctgcact
      781 actgcctgga gcgtgaaatc ccgttcctgt acgcttcttc cgcagccacc tacggcggac
      841 gcacctccga ctttattgaa tcccgcgagt acgaaaaacc gttgaacgtc tacggttact
      901 caaaattcct gtttgatgaa tatgttcgcc aaatcctgcc ggaagcgaac tcgcagattg
      961 ttggcttccg ctatttcaac gtttatggac cgcgtgaagg ccataaaggc agcatggcga
     1021 gcgtcgcttt ccatctcaac acccagctta ataacggtga atcaccgaag ctgttcgaag
     1081 gtagcgagaa ctttaaacgc gacttcgtct atgttggcga tgtggcagat gtaaacctgt
     1141 ggttcctgga aaatggcgtt tccggcatct tcaacctcgg caccggtcgt gcggaatcct
     1201 tccaggctgt agctgatgct acgctggctt atcacaagaa aggccagatc gaatacattc
     1261 cgttcccgga taagctgaaa ggccgctacc aggcgttcac tcaggcagat ctgacaaatc
     1321 tgcgcgcggc gggttacgac aaaccgttca aaaccgttgc tgaaggtgta acggaataca
     1381 tggcctggct gaatcgcgac gcataagagc tctgcatgaa aatactggtg atcggcccgt
     1441 cttgggttgg cgacatgatg atgtcgcaaa gtctctatcg cacgctccag gcgcgctatc
     1501 cccaggcgat aatcgatgtg atggcaccgg catggtgccg tccattatta tcgcggatgc
     1561 cggaagttaa cgaagctatc cctatgcctc tcggtcacgg agcgctggaa atcggcgaac
     1621 gccgcaaact gggtcatagc ctgcgtgaaa agcgctacga ccgcgcctac gtcttaccta
     1681 actccttcaa atccgcatta gtgcctttct tcgcgggtat tcctcatcgc accggctggc
     1741 gcggcgagat gcgctacggt ttactcaacg atgtacgcgt gctcgataaa gaagcctggc
     1801 cgctaatggt ggaacgctat atagcgctgg cctatgacaa aggcattatg cgcacagcac
     1861 aagatctgcc gcagccattg ttatggccgc agttgcaggt gagcgaaggt gaaaaatcat
     1921 atacctgtaa tcaattttcg ctttcatcag aacgtccgat gattggtttt tgcccgggtg
     1981 cggagtttgg tccggcaaaa cgctggccac actaccacta tgcggagctg gcaaagcagc
     2041 tgattgatga agattatcag gtggttctgt ttggctcggc gaaagatcat gaagcaggca
     2101 atgagattct tgccgcttta aataccgagc agcaggcatg gtgtcggaac ctggcggggg
     2161 aaacacagct tgatcaagcg gttatcctga ttgcagcctg taaagccatt gtcactaacg
     2221 attctggcct gatgcacgtt gcggcggcgc tcaatcgtcc gctggttgcc ctgtatggtc
     2281 caagtagccc ggacttcaca ccgccgctat cccataaagc gcgcgtgatc cgtctgatta
     2341 ccggctatca caaagtgcgt aaaggtgacg ctgcggaggg ttatcaccag agcttgatcg
     2401 acattactcc ccagcgcgta ctggaagaac tcaacgcgct attgttacaa gaggaagcct
     2461 gacggatgcg ggttttgatc gttaaaacat cgtcgatggg cgatgttctc catacgttgc
     2521 ccgcactcac tgatgcccag caggcaatcc cagggattaa gtttgactgg gtggtggaag
     2581 aagggttcgc acagattcct tcctggcacg ctgccgttga gcgagttatt cctgtggcaa
     2641 tacgtcgctg gcgtaaagcc tggttctcgg cccccataaa agctgaacgc aaagcgtttc
     2701 gtgaagcgct acaagcagag aactatgacg cagttatcga cgctcagggg ctggtaaaaa
     2761 gcgcggcact ggtgacacgt ctggcgcatg gcgtaaagca tggattggac tggcaaaccg
     2821 ctcgcgaacc tttagccagc ctgttttaca attgtaagca tcatattgca aaacagcagc
     2881 acgccgtaga acgcacccgc gaactgtttg ccaaaagttt gggctatagc aaaccgcaaa
     2941 cccagggcga ttatgctatc gcacagcatt ttctgacgaa cctgcctaca gatgctggcg
     3001 aatatgccgt atttcttcat gcgacgaccc gtgatgataa acactggccg gaagaacact
     3061 ggcgagaatt gattggttta ctggctgatt caggaatacg gattaaactt ccgtggggcg
     3121 cgccgcatga ggaagaacgg gcgaaacgac tggcggaagg atttgcttat gttgaagtat
     3181 tgccgaagat gagtctggaa ggcgttgccc gcgtgctggc cggggctaaa tttgtagtgt
     3241 cggtggatac ggggttaagc catttaacgg cggcactgga tagacccaat atcacggttt
     3301 atggaccaac cgatccggga ttaattggtg ggtatgggaa gaatcaagta gagtgtcgtt
     3361 ctacaagtat gtctcttgca gatttgccag ctcaaaccgt ttttcaaaat ttaaatcttg
     3421 aaataataac caataagttg acatcggaga taagatgacc tcaacattat ttttctctct
     3481 cgagaaaaaa aactggatag cgtactggaa cagagctctc gtattcttat tcattaccac
     3541 ctattttttg ggtgggatta caaggtataa acatcttatt gttattctta tgacaataac
     3601 gacaatcgtc tatctctgca aacggccaaa acactatctc tcactattta aaacatttct
     3661 ttttggtagt gttgccatat taactattgc tgcattgctg tcacttcttc aatcccctga
     3721 tgcaggtgct agcatgaagg aaattttcaa agctattatt gagaatactt tactatgcac
     3781 aatagcaata ccgatcatat tgagagacga gaaaagagaa gatgtcgaaa aaatcgtttt
     3841 cttctcattt attagtgcgt tgggcttacg ctgtttttct gaattgatta cctattataa
     3901 ggactatcaa caagggataa tgccattcgc agattataga caccgtagca tttctgactc
     3961 gatggtcttt ttattccctg cattgttaaa tctctggctt atcaaatcag caaaataccg
     4021 catttctttt gtggttctaa gcgttatttt tatttttctg atattaggaa ctttatccag
     4081 aggggcctgg ctttccgtgt tagtcattgg attaatatgg attctgatgt ttaaacaatg
     4141 gaagttacta ttagtaggag taatggttgc catcattgca ttgtcggtta ttttcacaca
     4201 taaggagatg accgcaaagc taacgtataa acttcaacaa actaatagtt cttatcgcta
     4261 tgcaaatggt actcaaggca gcgcactcga tctaatatta gaaaatcctg ttattggtta
     4321 tggttacggt aacgttgcat ataaagatgt ctataataaa cgtgtcattg attatccaga
     4381 atggaccttt agacaatcaa tagggccaca taattttgcg ctattcatct ggtttggcac
     4441 tggtttatta gggctggtaa gtcttatgat gctatactgt gcaatattga aagagtgtat
     4501 aaaaaatggc gtcaagaata aatatcgctc accatataat gcatattata taatcttact
     4561 atcttttata
//
LOCUS       DAZKIV010000238         4505 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZKIV010000238 DAZKIV010000000
VERSION     DAZKIV010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4505
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.240"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..180
                     /gene="grlA"
                     /locus_tag="ACHOT6_004551"
     CDS             <1..180
                     /gene="grlA"
                     /locus_tag="ACHOT6_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE transcriptional
                     regulator GrlA"
                     /protein_id="HFP9949639.1"
                     /translation="GNLHYKRLEIFIYDVNLEAVPIESPGSTGPKRKTYRVGNGIVGQ
                     SNIWNEMILRRKKES"
     gene            complement(564..1019)
                     /gene="cesD"
                     /locus_tag="ACHOT6_004552"
     CDS             complement(564..1019)
                     /gene="cesD"
                     /locus_tag="ACHOT6_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone CesD"
                     /protein_id="HFP9949640.1"
                     /translation="MSRKFSSLEDIYDFYQDGGTLASLTNLTQQDLNDLHSYAYTAYQ
                     SGDVITARNLFHLLTYLEHWNYDYTLSLGLCHQRLSNHEDAQLCFARCATLVMQDPRA
                     SYYSGISYLLVGNKKMAKKAFKACLMWCNEKEKYTTYKENIKKLLGNTE"
     gene            complement(1033..2571)
                     /gene="escC"
                     /locus_tag="ACHOT6_004553"
     CDS             complement(1033..2571)
                     /gene="escC"
                     /locus_tag="ACHOT6_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE outer membrane
                     ring protein EscC"
                     /protein_id="HFP9949641.1"
                     /translation="MKKISFFIFTALFCCSAQAAPSSLEKRLGKNEYFIITKSSPVRA
                     ILNDFAANYSIPVFISSSVNDDFSGEIKNEKPVKVLEKLSKLYHLTWYYDENILYIYK
                     TNEISRSIITPTYLDIDSLLKYLSDTISVNKNSCNVRKITTFNSIEVRGVPECIKYIT
                     SLSESLDKEAQSKAKNKDVVKVFKLNYASATDITYKYRDQNVVVPGVVSILKTMASNG
                     SLPSTGKGAVERSGNLFDNSVTISADPRLNAVVVKDREITMDIYQQLISELDIEQRQI
                     EISVSIIDVDANDLQQLGVNWSGTLNAGQGTIAFNSSTAQANISSSVISNASNFMIRV
                     NALQQNSKAKILSQPSIITLNNMQAILDKNVTFYTKVSGEKVASLESITSGTLLRVTP
                     RILDDSSNSLTGKRRERVRLLLDIQDGNQSTNQSNAQDASSTLPEVQNSEMTTEATLS
                     AGESLLLGGFIQDKESSSKDGIPLLSDIPVIGSLFSSTVKQKHSVVRLFLIKATPIKS
                     ASSE"
     gene            complement(2571..3026)
                     /gene="sepD"
                     /locus_tag="ACHOT6_004554"
     CDS             complement(2571..3026)
                     /gene="sepD"
                     /locus_tag="ACHOT6_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE switch protein
                     SepD"
                     /protein_id="HFP9949642.1"
                     /translation="MNNNNGIAKNDCDWLTALDFVKDVNGSPTHLTFYIYQKNAFLHD
                     FGNYWVLYIELSGDFRQVPTDTFIRLCNILAVSKEYKQMGIFLSNKKWYLCQIFHKDN
                     NHRANMSKAIMQHTLASLLDKQFDKLEQSSSSDTMMPPTHLFSDIGRIV"
     gene            complement(3032..3604)
                     /gene="escJ"
                     /locus_tag="ACHOT6_004555"
     CDS             complement(3032..3604)
                     /gene="escJ"
                     /locus_tag="ACHOT6_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscJ"
                     /protein_id="HFP9949643.1"
                     /translation="MKKHIKNLFLLAAICLTVACKEQLYTGLTEKEANQMQALLLSND
                     VNVSKEMDKSGNMTLSVEKEDFVRAITILNNNGFPKKKFADIEVIFPPSQLVASPSQE
                     NAKINYLKEQDIERLLSKIPGVIDCSVSLNVNNNESQPSSAAVLVISSPEVNLAPSVI
                     QIKNLVKNSVDDLKLENISVVIKSSSGQDG"
     gene            complement(3607..3984)
                     /gene="sctI"
                     /locus_tag="ACHOT6_004556"
     CDS             complement(3607..3984)
                     /gene="sctI"
                     /locus_tag="ACHOT6_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HFP9949644.1"
                     /translation="MNNINQSENINIQLNKASQTNVVDEHIPLASTPSAAGAAQFLDQ
                     LLPKTAGVSSPEQVLIEEIKKRHLATMNTDLSFDALSAGGLSPEDVLTLQKNVLNANV
                     NVDVVSKLASLLSTSVTKLVSMQ"
     gene            complement(4065..4370)
                     /locus_tag="ACHOT6_004557"
     CDS             complement(4065..4370)
                     /locus_tag="ACHOT6_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SepZ/EspZ family type III secretion system
                     protein"
                     /protein_id="HFP9949645.1"
                     /translation="MEAANLSPSGAVLPLAATINGNNPVDEKTGVMQSEGGTSRSVRI
                     LGGVLIGAGVLAAIGTGIAAMCVDDPSQTSQRLGLGIAAGVLGGVTTVAGGLAMKYA"
BASE COUNT         1295 a          842 c          856 g         1512 t
ORIGIN      
        1 ggtaatttgc attataagcg ccttgagatt ttcatttatg atgttaacct tgaggcggtt
       61 ccgatagaaa gtcctggatc aaccggacca aaaagaaaaa cctaccgagt tggtaatggt
      121 attgtgggac agtctaatat ctggaacgaa atgatcttga ggaggaaaaa ggagagttag
      181 acgtaatttt ttgagtatat tttatagaag aataaaaata aagctatatt taagggtttt
      241 gttttccagg gtaagccttt ttctcggcta aagtttgaag gtcatttcct gacaaatata
      301 ctctgagcta tagatgattg gaaagatcag atttctaatg ctaagttaag atatactgat
      361 tgtttcataa acggaaatga tattaaatac agcagaagat agttcagggc ataaaatcct
      421 ggttaatgga ataaccccac acctgtagat aaatctacca gtggtcggtg ctcaactttg
      481 ggagcacttc actatagcag ctttattatt tccgatagaa aatccccagt cagcctgggg
      541 attggtatag ctgatggtag tttttactct gtattaccta acaatttttt aatattttct
      601 ttatatgtag tgtatttttc tttttcatta caccacatta aacaagcctt aaaggctttc
      661 ttggccattt tcttatttcc gacgagtaag tagctaattc cagaataata agatgccctg
      721 ggatcttgca taactaaagt tgcacagcgt gcgaaacaca gttgtgcatc ttcatgattt
      781 gataaacgct gatggcataa gcccagagat aaggtgtagt cataattcca gtgttccaga
      841 tatgtgagca aatggaatag atttcttgcg gttataacat caccagattg atatgctgta
      901 taggcataag aatgaaggtc attgagatct tgttgtgtca gatttgttaa tgacgctaat
      961 gtgccaccat cctggtagaa atcataaata tcctctagag agctaaattt cctgctcata
     1021 aagcctcatt tattattcgc tagatgcaga ttttatcggg gttgctttaa ttaaaaagag
     1081 tcgaacaaca ctatgttttt gttttaccgt actggagaat aaactaccaa taacaggaat
     1141 gtcgcttaac agcggtatac catctttact tgaactttct ttatcttgga taaatccgcc
     1201 aagtaataaa ctttctcctg cacttaatgt cgcttctgtt gtcatttctg aattttgtac
     1261 ttcaggtaag gtgctgctgg catcctgagc attgctttgg ttagtcgatt gattgccatc
     1321 ctgaatatca agtaacaaac gaactcgttc ccgacgtttt ccggtaagac tattcgagct
     1381 gtcatcaagt atacgtggtg tcactctcag caatgttccg cttgtaatac tttccagaga
     1441 cgccaccttt tctccggaga cttttgtata aaacgttacg tttttatcaa gaatcgcctg
     1501 catattattt aaggttataa ttgacggctg agagagtatt ttagctttcg agttctgttg
     1561 taacgcattc accctaatca taaaattact ggcattactt atcacagaag agctgatatt
     1621 agcctgtgcg gtagagctat taaaggcaat cgtaccttgt cctgcattta acgtaccgga
     1681 ccagtttacg ccgagctgct gaagatcgtt tgcatcaaca tcaataattg agacagaaat
     1741 ttcaatttgc ctttgttcaa tatcaagctc agaaattaat tgctgataga tatccatcgt
     1801 tatttcgcgg tcttttacaa caactgcatt aagacgtgga tcagcggaaa tcgttacact
     1861 gttatcaaac aggttaccgc ttcgctcgac ggcaccttta ccggtactgg gaagcgaacc
     1921 attacttgcc attgtcttca gaatggagac aacacctggg acgacaacat tttggtcacg
     1981 gtatttgtat gttatatctg tcgctgacgc atagttcagt ttaaagacct taacaacatc
     2041 tttatttttg gctttagact gtgcctcttt atcaagactt tctgataaag atgtaatata
     2101 ttttatacat tcaggaacac cccgaacttc aatcgagtta aatgtcgtaa tttttctcac
     2161 attgcaagag tttttgttta ctgagattgt atcactcaga tattttagca gtgagtcaat
     2221 atccagatat gttggtgtaa ttatacttct tgatatttca tttgttttat aaatatacaa
     2281 tatgttctca tcataatacc atgtgagatg atagagtttg gacagttttt ccagtacttt
     2341 tacaggtttt tcatttttta tttcgccaga gaaatcatca ttcacggaac tactgatgaa
     2401 aacaggaatg ctgtagttag cagcaaagtc attaaggatt gcacgtactg gagaactttt
     2461 agtaataata aaatattcgt tcttgcctaa tcttttttcc agagaggacg gggcagcttg
     2521 tgcactgcag caaaatagtg ctgtaaaaat aaaaaaactt atttttttca ttacacaatt
     2581 cgtcctatat cagaaaacaa gtgtgttggc ggcatcattg tatctgatga agatgattgc
     2641 tctagtttat caaattgctt gtcgagcaac gaggcgagag tatgttgcat tattgctttt
     2701 gacatgtttg cgcgatgatt attatcttta tgaaaaattt ggcaaagata ccacttctta
     2761 ttagacagga aaatacccat ttgtttatat tctttactaa cggccaggat attgcagagt
     2821 ctgataaatg tatctgttgg aacctgacga aaatcaccac taagctcgat atatagaacc
     2881 cagtagtttc caaaatcgtg caagaaagca tttttttggt agatataaaa agtgagatgc
     2941 gttggtgaac cgttgacatc ctttacaaaa tcaagggccg ttagccagtc acaatcattc
     3001 tttgctatgc cattattatt gttcatacat attacccgtc ctgtcctgag gatgacttga
     3061 taacaacact aatattttct aatttgagat cgtcgacact attcttaacc aggtttttta
     3121 tctgaatgac cgatggtgct aagttaacct ctggcgagct gatgactaaa acggctgctg
     3181 aggatggttg actttcgtta ttgttaacat ttaatgaaac gctgcaatcg ataacaccgg
     3241 gtattttact tagcaacctt tcgatgtctt gttctttgag atagttgatt tttgcatttt
     3301 cttgtgatgg cgatgccact aactggctgg gtgggaaaat aacctcgata tctgcaaatt
     3361 tctttttcgg gaagccgtta ttatttaaaa tagttattgc gcgaacaaaa tcctcttttt
     3421 ccacactgag tgtcatattt ccacttttgt ccatttcttt ggaaacattg acatcattgg
     3481 atagcaacag tgcttgcatc tgattagctt ctttttctgt taatcctgta tacaattgct
     3541 ctttacatgc cactgtcaaa caaatagcag ccaataaaaa aaggttttta atgtgttttt
     3601 tcatttttat tgcatcgaaa ctaattttgt aaccgatgtt gaaaggaggc ttgctaactt
     3661 agatacgaca tcaacattga cgtttgcgtt gagcacattc ttttgtaaag tgagtacatc
     3721 ttcgggcgag agcccacctg cagatagtgc atcgaaactg agatcggtgt tcattgttgc
     3781 aagatgtcgc tttttaattt cttcaatcaa cacttgttct ggggaagaca ctcccgccgt
     3841 tttagggagt aattgatcaa gaaactgagc cgctccggct gcagaggggg ttgatgctaa
     3901 cgggatatgc tcatcaacga catttgtttg ggatgcttta tttaactgaa tattaatgtt
     3961 ttccgattga ttaatattat tcatatttac tgttagtctc ttttcatgag agaggcaata
     4021 gcataatgca tccattacct ctctattttt atgcgatgcg atgattaggc atatttcatc
     4081 gctaatccgc cggcaacagt agttacgcca cctaatacac cggctgcgat tcctaagcct
     4141 aatctttgtg aagtttgtga agggtcgtca acacacattg ctgcgatacc agtaccgata
     4201 gcggctagaa cacctgcacc tattaaaact ccacccagga ttctaacgct gcgacttgtt
     4261 ccaccttctg actgcatcac ccctgtcttc tcatccactg gattatttcc attgattgtg
     4321 gctgccagtg gtaatactgc accagaaggg cttaaatttg ctgcttccat tgatctttct
     4381 ccttttgtct gatttagaca tttacctggt tataaataac caaattatcc cataggcaac
     4441 tattatatta tttaaatagt tgcaatgtag ataaagtaca tatactgtat atattatcca
     4501 atacg
//
LOCUS       DAZKIV010000239         4430 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZKIV010000239 DAZKIV010000000
VERSION     DAZKIV010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.241"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            230..1387
                     /gene="acrE"
                     /locus_tag="ACHOT6_004558"
     CDS             230..1387
                     /gene="acrE"
                     /locus_tag="ACHOT6_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFP9949646.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            1399..4337
                     /gene="acrF"
                     /locus_tag="ACHOT6_004559"
                     /pseudo
     CDS             1399..4337
                     /gene="acrF"
                     /locus_tag="ACHOT6_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
BASE COUNT         1092 a         1070 c         1163 g         1105 t
ORIGIN      
        1 tttcaattta cgaggtttta attctgcctc tttcaacccg cgtcaaaata aaacagtaga
       61 atattaatct ttttttgtgt ttatgtgcct tgagacgcca gtattcataa ctattcctta
      121 catcgacgaa tgataatttg taggatagca aactgtattt ttctttctgc gagttaacgc
      181 gttgcctttc tgggtaaata acgcgctttt ggttttttga ggaatagtaa tgacgaaaca
      241 tgccaggttt ttcctcctgc cctcctttat tctgatctcc gcggctttaa tcgccggttg
      301 taacgataag ggagaagaga aagctcacgt cggtgaaccg caggttaccg ttcatattgt
      361 gaaaacggcc ccgttagaag ttaagactga attaccaggc cgcaccaatg cttaccgtat
      421 agccgaagtt cgcccacagg ttagcgggat cgtactgaat cgtaatttca ctgaaggcag
      481 cgatgtacaa gcaggccagt ccctgtacca gatcgatccc gcgacctatc aggcaaatta
      541 tgacagcgcg aaaggcgaac tggcgaaaag tgaagccgcc gccgccatcg cgcatttgac
      601 ggtaaaacgt tacgttccgc tcgtgggtac gaaatacatc agccagcagg agtacgacca
      661 ggccattgct gatgctcgcc aggccgatgc cgccgtgatt gccgcaaaag ccacagtcga
      721 aagcgctcgc atcaatctcg cttataccaa agtgactgcg ccaattagcg gacgtatcgg
      781 caaatcgacc gtgaccgaag gcgctcttgt cactaatggg caaacgactg aactggcgac
      841 agtccagcag ctcgatccta tctacgttga tgtgacccaa tccagcaacg attttatgag
      901 gctgaagcaa tccgtagagc aaggaaattt gcataaggaa aacgccacca gcaacgtaga
      961 gttggtcatg gaaaacggtc aaacctatcc cctgaaaggt acgctgcaat tctctgatgt
     1021 gaccgttgat gaaagcaccg gctccataac cctacgtgct gtcttcccta acccgcaaca
     1081 tacgcttttg ccgggtatgt ttgtgcgtgc acggattgat gaaggcgtcc aacctgacgc
     1141 cattcttatc ccgcaacaag gcgttagccg cacaccgcgt ggtgatgcaa ccgtgctgat
     1201 tgttaacgat aaaagtcagg tggaagcgcg ccctgtcgtt gccagtcagg cgattggcga
     1261 taaatggttg attagcgaag gtctgaaatc tggcgatcaa gtcattgtca gcggcctgca
     1321 aaaagcgcgt ccgggagagc aggttaaagc cactaccgat acccccgcag atactgcatc
     1381 gaagtaaggt aatctgacat ggcaaacttt tttattcgac gaccgatatt tgcatgggtg
     1441 ctggccatta ttctgatgat ggcgggcgca ctggcgatcc tacaattgcc cgtcgctcag
     1501 tatccaacca ttgcaccgcc tgcggtttct gtttcggcaa actatccagg tgctgatgcg
     1561 cagaccgtgc aggatacggt gacgcaggtt atcgaacaga atatgaatgg tatcgataac
     1621 ctgatgtata tgtcctccac cagcgattcc gccggtagcg tgacaattac ccttaccttc
     1681 cagtccggga ccgatcctga tatcgcgcaa gtgcaggtgc agaacaaact ccagctcgcc
     1741 acgccgttgc tgccgcagga ggttcagcag caggggatca gtgttgaaaa gtccagtagc
     1801 agctatttga tggtggcggg ctttgtctct gataacccag acaccacaca ggacgatatc
     1861 tcggactatg tggcctctaa cgttaaagat acgcttagcc gtctgaatgg cgtcggtgac
     1921 gtacagcttt tcggcgcaca gtatgcgatg cgtatctggc tggacgccga tctgctaaac
     1981 aaatataaac tgacaccggt tgatgtgatt aaccagttga aggtacagaa cgatcagatc
     2041 gctgccggac agttgggcgg aacgccagcg ttaccagggc aacagttgaa tgcctcgatt
     2101 attgctcaaa cgcggttaaa aaatccggaa gaattcggca aagtgaccct gcgcgtaaac
     2161 agtgacggct cggtggtacg cctgaaagat gtcgcacggg ttgaacttgg cggtgaaaac
     2221 tataacgtta tcgctcgtat caacggaaaa ccggcggcgg gcctggggat taagctggca
     2281 accggcgcga atgctctcga taccgcgaaa gccattaagg caaaactggc ggaattacag
     2341 ccattcttcc cgcagggaat gaaggttctc tacccttatg acaccacgcc attcgtccag
     2401 ctttctattc acgaagtggt aaaaacgctg ttcgaagcca ttatgctggt gttcctagtg
     2461 atgtatctgt tcttgcagaa tatgcgagca acgctgatcc ccaccattgc ggtacccgtg
     2521 gtgttattag ggacgtttgc catcctcgcc gcttttggtt actccatcaa cacactaacg
     2581 atgttcggga tggtgcttgc catcgggctg ctcgtcgatg atgcgatagt ggtggtggag
     2641 aacgtcgagc gcgtgatgat ggaggataag ctcccgccaa aagaagcgac ggaaaaatcg
     2701 atgtcgcaaa ttcagggcgc actggtgggt atcgcgatgg tgctgtcagc ggtatttatt
     2761 ccgatggcat tcttcggctg gtttaatacc accttcgatc atagcgttaa ccactacacc
     2821 aacagcgtcg gcaaaatcct cggttccaca ggacgatatt tactgatcta tgcgctgatt
     2881 gttgcaggaa tggtggtgtt gtttttacgt cttccttctt acctgaagag gatcagggtg
     2941 tctttctgac catgattcag ttacccgctg gcgcgacgca agagcggacg caaaaagtgt
     3001 tggatcaagt gacggattac tatctgaaga acgagaaagc gaacgttgaa agtgtcttta
     3061 cggttaacgg ctttagcttc agcggccagg cacaaaacgc cggtatggcc ttcgtcagtc
     3121 tgaaaccgtg ggaagagcgt aatggtgatg aaaacagtgc ggaagcggta atccatcgtg
     3181 ccaaaatgga attgggcaag atccgcgacg gttttgtcat tccattcaat atgccagcca
     3241 ttgttgaact gggcacggca acgggtttcg actttgagtt aattgatcag gctgggctgg
     3301 gtcacgatgc cctgacccag gcccgtaacc agttgcttgg catggcggcg caacatcctg
     3361 ccagcttagt cagcgtgcgt cctaatggcc tggaagacac cgcgcagttt aaactggaag
     3421 ttgaccagga aaaggcgcag gcattaggtg tttcactttc tgacatcaat cagaccattt
     3481 caacggcgtt gggtgggact tacgttaacg acttcatcga ccgtggtcgc gtgaaaaagg
     3541 tgtatgttca ggcggatgcc aaattccgta tgctgccaga agatgtcgat aaactttatg
     3601 tccgcagcgc caacggcgaa atggtgccat tctcggcctt taccacttca cattgggtgt
     3661 atggctctcc gcgactggaa cgctacaacg gtctgccgtc aatggagatt cagggggaag
     3721 ccgcgccagg aaccagttcc ggcgatgcca tggcgttgat ggaaaacctt gcgtcaaaat
     3781 tacctgcggg cattggttat gactggacgg gtatgtcgta tcaggaacgc ttatcgggaa
     3841 accaggctcc cgctctggta gcaatttcct ttgtggttgt tttcctgtgc cttgctgcac
     3901 tctatgaaag ctggtcaatt cctgtctcgg ttatgttggt cgtgccgtta gggattgtcg
     3961 gcgtgctgct ggcggcgaca ctctttaatc aaaaaaatga cgtctacttt atggtgggct
     4021 tgctaacgac aattggcttg tcggccaaaa acgctatttt gattgttgag ttcgctaaag
     4081 atctcatgga gaaagagggt aaaggtgttg ttgaagcgac actaatggca gtacgtatgc
     4141 gtctgcgtcc tatcctgatg acctctctcg cctttattct cggcgtatta ccgctagcta
     4201 tcagtaacgg tgccggcagt ggcgcgcaga acgcagtggg tatcggggta atgggcggaa
     4261 tggtctctgc aacgttgctg gcaatcttct tcgtaccggt gttctttgtg gtgatccgcc
     4321 gttgctttaa aggataaatc agaaacataa aggcactttc gggtgccttt attattttca
     4381 gtgaaaccca taaaaattaa taagatattc ttccactcac tctttaaaaa
//
LOCUS       DAZKIV010000240         4404 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZKIV010000240 DAZKIV010000000
VERSION     DAZKIV010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.242"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..194)
                     /gene="gpU"
                     /locus_tag="ACHOT6_004560"
     CDS             complement(<1..194)
                     /gene="gpU"
                     /locus_tag="ACHOT6_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9949647.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHI"
     gene            complement(191..769)
                     /locus_tag="ACHOT6_004561"
     CDS             complement(191..769)
                     /locus_tag="ACHOT6_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949648.1"
                     /translation="MAIKGLEQAIENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKARRKLVKERARLKRTTVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            complement(781..1134)
                     /locus_tag="ACHOT6_004562"
     CDS             complement(781..1134)
                     /locus_tag="ACHOT6_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9949649.1"
                     /translation="MADFDNLFDAAIVRADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(1146..1541)
                     /locus_tag="ACHOT6_004563"
     CDS             complement(1146..1541)
                     /locus_tag="ACHOT6_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9949650.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDGAGQDSSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1583..2608)
                     /locus_tag="ACHOT6_004564"
     CDS             complement(1583..2608)
                     /locus_tag="ACHOT6_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9949651.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSATN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     THARGLRTYGCIQDADALSEGINASPRYPKNWKTSGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2664..2996)
                     /locus_tag="ACHOT6_004565"
     CDS             complement(2664..2996)
                     /locus_tag="ACHOT6_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9949652.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(3006..4325)
                     /locus_tag="ACHOT6_004566"
     CDS             complement(3006..4325)
                     /locus_tag="ACHOT6_004566"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9949653.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQACVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(4306..>4404)
                     /locus_tag="ACHOT6_004567"
     CDS             complement(4306..>4404)
                     /locus_tag="ACHOT6_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949654.1"
                     /translation="AAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          891 a         1361 c         1176 g          976 t
ORIGIN      
        1 tcaatatgca gctccgcctg ccaggtatcg ctgtccagct cttcgcccgt gtattcagcg
       61 ccggtgagat aaacggcaac tgccggaaaa tccgcctcat caaaaacagc ggggcgacca
      121 tcaaaaagcg tcgccccggt gtcatgcttc tccagtgcat ccagtacggc tgcacggagt
      181 tcagtatgtt tcatcgcttt attaccattc tcagttgatg ctgcagcgca tagcccagct
      241 ctttcggaag acgttcacgc cgtatccgtt caatattctg tttaaacgcc gtggtaagcg
      301 gcaccgccat cgggattttc accacatcaa tggggtaacg gtttttcccg gccacacgct
      361 gcatgacatg ccaccgccca tttttcagtt gctgaataaa cgcgccggga atacgacggt
      421 tacccaccac aagcacgctg ccgccacctt tcagggatga acgctgcccc tttttacgac
      481 gcctgcgtcg ggacaggaca acccgcgcat tccccagctt gattacgggc aaatcccccc
      541 ggttaacttt gattctggcc tgcggatttt tgaccgtggt ccttttcagc ctggcccttt
      601 cctttaccag tttccggcgt gcctttgtct cacgggcaac ctgtgacgcc gactgcgata
      661 tcgcggatga agcaacgcgg ttaatggcca ttgcggcggc accaggcacc gccgttttgc
      721 tgatacggct gaggttttca atggcctgct caagaccttt tatggccata catccccctt
      781 tcagcggcga cggttaacgg caggcggtac gccccgccca agccagagat gacagcttcc
      841 accatcatcc ggcgaaaccc ggtctatcca gaagttttcc tcaccgatgg tcagcgtgtc
      901 tccacgccgc agctgccgca catcatcagt ccggacaaac agggacgggc tggagccttc
      961 aacgcgcacg ccctgtccgg catagctgat attttcaggg tcatcaaaaa caccacgtat
     1021 tactgcgccg gactgctcac cggatgtcat ggtggctgac gttcccatgt acccgcgtat
     1081 cgtttcatcg gcgcggacaa tggcagcatc gaacaggtta tcgaaatcag ccacagcgcc
     1141 tcccgttatt gcattctggc caggccgcgc tctgtcattt cggctgccac accggcagag
     1201 acacgaaacg ccgttcccgg cagcacaaat gccacaggtt catcccgcgt ggcgtgaagt
     1261 gcatcggtat gcagcgtcac cagtgccaca accgtgacca gagcagccgt atcagtcacg
     1321 gtatccgtct gtgctgatac cacctcattt tcatgtccgg tcagcgcatt ttccgggctg
     1381 acagacgagt cctgaccggc tccgtcatcc gtgtcatcaa gctcctcttc cagctctgcc
     1441 acacggagcg ccagttcttc tttcgtcccc gtcaggctga catcacggtt cagttgctca
     1501 cccagcgacc ggagacgggc aatcagttca tctttcgtca tggactcctc cacagagaga
     1561 aaatggcccc taagggccat gattacgcca gttgaacgga cacgaactca tcaggatcag
     1621 ccagcagcat cagcggtgct gactgaatca tggtgaactc tcgcgccgga tcgccggatg
     1681 tcttccagtt tttcggataa cggggagacg cattaatacc ctcactcaat gcatccgcat
     1741 cctgaataca gccataggtg cgcagaccgc gtgcatgagt gttacccagc accatcgtgt
     1801 tgtccggcag gaagttcttt ttgacgccgt tttccacgta ctgtccggaa tacacgacga
     1861 tggccacatc gccatacatt cccttataag acaccgcttt gcccaggtct tttaccgctg
     1921 tctccagttc ggaatgagag ccgcgacggg tatccagctt ctccctgacg gctttgaagg
     1981 aacggaacag cgcccagcct ttcgggtcaa acacgatgat attcaccacg ccgctggcgt
     2041 tcagcgcgta ggcttcgata tcgtcggtcg ggtcatacgt ggacttgtca cgcttgctcc
     2101 actccgtgcc gccggactgc gtgatgttgt tcgtcgcact gcggcccata tccacctcaa
     2161 ccggatcgaa ggcttcaccg gtcatggtgt atttgccctt gagcacggca gaaactgcct
     2221 gcatctcttc gacctgagca atggccagct cttcgtcacg catgttctgc atgatgatgc
     2281 gacggcggcg gtaagccggg tccgccagat tctgcggatc ttcatccggc aggcgacgca
     2341 gggtcatctg cggattcact tcatgcttgg gtttgacata tcccggcgta aattcagagg
     2401 tggagccgcc acgggaacgg ataacctcac cggaaacaat cggcgaaacg tacagcgcca
     2461 tgtttaccag tcccggaatt tgtgagagat agactttctc cgtagtgaag ggatagctct
     2521 cacggaaaaa gagacgcaga aacagcggat caaacttaaa tttctgctca tttgccgcca
     2581 gcagctgggc ggttgtgtac atcgacataa aaaaatcccg taaaaaaagc cgcacaggcg
     2641 gcctttagtg atgaagggtc aggttaaacg atgctgattg ccgttccggc aaacgcggtc
     2701 cgttttttcg tctcgtcgct ggcagcctcc ggccagagca catcctcata acggaacgtg
     2761 ccggacttgt agaacgtcag tgtggtgctg gtctggtcag cagcaactgc aagaatgcca
     2821 acggcagcac cgtcggtggt gccatcccac gcaaccagct tacgggtgga ggtgtccagc
     2881 atcagcgggg tcattgcagg cgctttcgca ctcaatccgc cgggcgcggt tgcggtatga
     2941 gccgggtcac tgttgcccag cggctggtaa tgggtaaagg tttctttgct cgtcataaac
     3001 atcccttaca ctggtgtgtt cagcaaatcg ttaacggcat cagatgccgg gttacctgca
     3061 gccagcggtg ccggtgcccc ctgcatcaga cgatccagcg cagtgtcact gcgcgcctgt
     3121 gcactctgtg gtgctgcggc cagaatacgg cgggccgttt ccacggtcat accgggggtt
     3181 tcggccagca cgcatgcctg ttcttcgcgt ccgtgagcct cctcacagtt gaggatcccc
     3241 ataatgcgac tgttttctgc cgcaaccgct gcggtgatct gcgcgttcac gtccggctgc
     3301 gcagcgctgg cgttctcgcc ctccgtcgct tgcaccacgc cagtaacgtc agcctgcgaa
     3361 gcagtggctg aaacagttgt tgattgagtc tctttggtca ttcgccctcc tgagagacgg
     3421 gatttacgtg catccagtgc atcacgcatg acggtgatcg catcggtact gttcacaagt
     3481 tcatcagcca gtccggcatc aatggcctcc tgaccgctgt acactgcagc ctcggtatcc
     3541 agcacagcct gcacggacag gccggtatat gccgacacct tctgtgcaaa catccggcgg
     3601 gttgcatcca tccgggactg cagtgtttcc cggacatcat ccggtagatg gctgtagggg
     3661 ttgccatcca ccttatggct gccgctgtaa atcagcgtga tttccacgcc ctgtttctcc
     3721 agcgcagcac cgtaattact gtgagccatc atgacgccga tggagcctgt ccgggcggtc
     3781 tgcgtgacca gacgccggga ggcggcgctg gcaagcagct gacctgcact gcagttcatg
     3841 tcgttggcca gcgcccatac cggttttatg tctcgcacac gggcaatgat gtcagcacag
     3901 tcaaatgctc ccgccaccat cccgcccggt gtgtccatat ccagcagaat gccgtccacc
     3961 atcggatcgc tggcagcctg ttgcagacgg gcgataatgc cgttgtaacc ggtcattccc
     4021 gaatacggct gcagcgcccg cgtccggctg accagcgtac cggacaccgg cagcacggcg
     4081 atgccgttca tgacctgata actgcgggcc tgtcgtggtc cgtcatcatc accggataac
     4141 gccagcgccg cgggtgcctc tccggcagtc aggctgtcgc cggatactgc atccgtcagg
     4201 cggctgatcc caagctggcc tgcaagcgca caaaagaaaa cccgcgcata ggcgggttca
     4261 agcatcagcg gctcattaaa agccatgctg gcaatatgcg ggagattacg cagctctgct
     4321 gtcactcttc tcctcctctg ttgattgtcg cagtccggat tcaaatgccg cagccgccca
     4381 ggcgggcggt ttaagaccgg ctgc
//
LOCUS       DAZKIV010000241         4399 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZKIV010000241 DAZKIV010000000
VERSION     DAZKIV010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.243"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1172)
                     /locus_tag="ACHOT6_004568"
     CDS             complement(<1..1172)
                     /locus_tag="ACHOT6_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9949655.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVP"
     gene            complement(1147..1692)
                     /gene="nohA"
                     /locus_tag="ACHOT6_004569"
     CDS             complement(1147..1692)
                     /gene="nohA"
                     /locus_tag="ACHOT6_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFP9949656.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAEREAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2081..2275
                     /locus_tag="ACHOT6_004570"
     CDS             2081..2275
                     /locus_tag="ACHOT6_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9949657.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIKSDEE"
     gene            complement(2526..2732)
                     /locus_tag="ACHOT6_004571"
     CDS             complement(2526..2732)
                     /locus_tag="ACHOT6_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949658.1"
                     /translation="MHKDQYTDAPSQEQVRVKTMLNSTISMGYPDVVIACIEHQVSLE
                     AFRAIEAALVKHDKNSKDYSLVVD"
     gene            complement(3028..3201)
                     /gene="gnsB"
                     /locus_tag="ACHOT6_004572"
     CDS             complement(3028..3201)
                     /gene="gnsB"
                     /locus_tag="ACHOT6_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFP9949659.1"
                     /translation="MNIEELKTKTEADISEYITKKIIELKKKTGKEVTSIQFTAREKM
                     TGLESYDIKIDLI"
     gene            complement(3374..3604)
                     /gene="ynfN"
                     /locus_tag="ACHOT6_004573"
     CDS             complement(3374..3604)
                     /gene="ynfN"
                     /locus_tag="ACHOT6_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnfN"
                     /protein_id="HFP9949660.1"
                     /translation="MKKIIVFFNSEPAMVVPVMTGVNTIMREYPNGETTHLTVMAAGF
                     PSLTGDHKVIYVAADRHVTSEEILEAAMRLLN"
     gene            complement(3636..3674)
                     /locus_tag="ACHOT6_004574"
                     /pseudo
     CDS             complement(3636..3674)
                     /locus_tag="ACHOT6_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518789.1"
                     /note="too short; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3677..3865)
                     /gene="ynfQ"
                     /locus_tag="ACHOT6_004575"
     CDS             complement(3677..3865)
                     /gene="ynfQ"
                     /locus_tag="ACHOT6_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFP9949661.1"
                     /translation="MTNHIHFRCPCCHGSQYRTSSFDVSDRNPFGAKCIFCKSMMITF
                     DNISQYLNVSRLSLDLRK"
     gene            complement(3876..4088)
                     /gene="cspI"
                     /locus_tag="ACHOT6_004576"
     CDS             complement(3876..4088)
                     /gene="cspI"
                     /locus_tag="ACHOT6_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspI"
                     /protein_id="HFP9949662.1"
                     /translation="MSNKMTGLVKWFNPEKGFGFITPKDGSKDVFVHFSAIQSNDFKT
                     LTENQEVEFGIENGPKGPAAVHVVAL"
BASE COUNT         1249 a         1064 c          891 g         1195 t
ORIGIN      
        1 tcaggaacgg gcgctgaata atgctctttc cgctctgcca tcacctcagc atccggacgt
       61 tcgccaattt tcgcttccca tgtctcaccg agcgtggtgt tcacgaaggt tttacgtttt
      121 cccgtatccc ctttcgtctt catccagtct ttgacaatct gcacccaggt ggtgaacggg
      181 ctgtacgccg tccagatgtg aaaggtcaca ctgtccggcg gctcaatctc ttcaccggat
      241 gacgaaaacc agagaatgcc atcacgggtc cagatcccgg tcttttcgca gatataacgg
      301 gcatcagtaa agtccagctc ctgctggcgg atgacgcagg cattatgctc gcagagataa
      361 aacacgctgg aggggtcatc cggcgtccat ttgaggccaa acggcgtctc tttgtcgcca
      421 aatttaaggt actgctcctc cccgcagtgc gggcaggcaa catgaaaacg cataaaatgc
      481 ggggattcac tggctgcacg ctcaatctgg caggtgcctc tcactttggg cgtggagcca
      541 cggatggact ttggccagac cgagccttca atacgtttgt cgccaaggaa cgtcggagag
      601 ccttcctgtt caatatcctc atcaaaggca gcaagttcat cataacccgc cacatccacc
      661 gacttttcac ggtagttttt tgccgcttta ccgcccaggc accagaagcc acgaccattg
      721 gaaaaacgct tcatagtgag cgtgttatcc cggtgctttt tgccatacca cggagccagc
      781 gccagcagcg acggaatatc gcggatggtc ggctcaacgt gggttttcat aaagttctcg
      841 gcatcaccat ccgtcggcaa ccagataagg gtgttgcgct gcttatgctc tatgaagtag
      901 gcataaacac ccagcagcat tttggaataa ccgacacggg cagacttcac cacattcacc
      961 tcacggatgt agtcgctgcc catcgcattc atgatggccc gctgaaaggg cagtgtttcc
     1021 cagcgccctt cctggtatgc ggattctttc gggagatagt aattagcatc cgcccattca
     1081 acggcggtct gtggctccgg cctgaacagt gagcgaagcc cggcgcggac aaaatgccgc
     1141 agcctgttaa cctgactgtt cgatatattc actcagcaac cccggtatca gttcatccag
     1201 cgcggctgct ttgttcatgg ctttgatgat atcccgtttc aggaaatcaa catgtcggtt
     1261 ttccagttcc ggaaaacgcc gctgcaccga cagggggatc ccgtcgagaa tactggcaat
     1321 ttcacctgcg atccgcgaca gcacgaaagt acagaatgcg gtttccacca cttcagctga
     1381 gtctctggca ttcttcagtt cctgtgcgtc ggcctgcgca cgcgtaagtc gatggcgttc
     1441 gtactcaata gtccctggct ggagatctgt ctcgctggcc tgccgcagtt cttcaacttc
     1501 ccggcgcagc ttttcgttct caatttcagc ctccctttcg gcataccatt ttataacggc
     1561 ggcagagtca taaagcacct cattaccctt gccaccgcct cgcagaacgg gcattccctg
     1621 ttcctgccag ttctgaatgg tacggatact cgcaccgaaa atgtcagcca gctgcttttt
     1681 gttgacttcc attgttcatt ccacggacaa aaacagagaa aggaaacgac aaaggccaaa
     1741 aagcccgttt tcagcacctg tcgtttcctt tcttttcagg gggtatttta aataaaaaca
     1801 ttaagttacg acgaagaaga acggaaacac cttaaaccgg aaaattttca taaatagcga
     1861 aaacccgcga ggtcgccgcc ccgtaaccgg tcggatcgcc ggaaaggacc cgcaaaatga
     1921 taataattat catctacatg tcacaacgtg catctacgcc atcaaaccac gtcaaataat
     1981 caattatgac gcaggtatcg tattaattga tctgcatcaa cttaacgtaa aaacaacttc
     2041 agacaataca aatcagcgac gctgaatacg ggacaacctc atgtcaacga agaacagaac
     2101 ccgcagaaca acaacccgca acatccgctt tcctaaccaa atgattgaac aaattaacat
     2161 cgctcttgat caaaaagggt ccgggaattt ctcagcctgg gtcattgaag cctgccgtcg
     2221 gagactaacg tcagaaaaga gagcatatac atcaatcaaa agtgatgaag aatgaatatc
     2281 ccggtttctt tacctcccct cccaccccgc acaggactgg cgagcgtgtg ggacaaatat
     2341 gtgagtcata aacgccttat cctcagaaat gcagttggca tccgaacagg acgatattgt
     2401 aaattcacct aattacgagg cattgcagta gttgaattgt agttctgtat tagcctgaca
     2461 gtgacagaat gcgatattga ctctgtcaca ggtgaaatag ttttaatggt tagcagttat
     2521 ggtgatcagt caaccaccag ggaataatcc ttcgaattct tatcgtgctt caccaacgct
     2581 gcctcaattg ccctgaatgc ttccagagac acttgatgtt ctatacatgc aattacaaca
     2641 tccgggtaac ccatcgaaat ggtgctatta agcatagttt ttacacgaac ctgctcctgg
     2701 gagggggcat cagtgtattg atctttatgc attttgtcgc tccatgcgtt tgctcttcac
     2761 ttgcggctaa aaatataacg tcaaatcact cagcatgaaa gagtgaaagt ttcagcgcgt
     2821 ttcccttaca taatctgtcg tttgtattct cttcaggcat accggaaaga ctaggtattg
     2881 tcacacaacc aataccatcg acaatcgaga tttatatcta ttcggtaatg tttaaatata
     2941 acaaaacccc gtaaaaacga ggtttatggg taatttgtat tgttgaatag catctgatga
     3001 gaaattgatg ctaatactat aaacatacta gattaaatca atcttgatat cgtagctttc
     3061 aagaccagtc attttttccc gtgcagtaaa ctggatactg gtaacttctt tcccggtctt
     3121 tttcttaagc tcaatgattt tttttgttat atattcagaa atatctgctt ctgtttttgt
     3181 ttttaactct tcaatgttca tcatttcctc ttttagtctg ttatgacttt cctggtacac
     3241 agtagtgtca attatatgga gcaaacgtat aaaagataag atgaaacatc gcaataatca
     3301 acatacgacg gtctaaattt cacacaaaca gataaagatg attatcattt attatcaatg
     3361 cattagaatc aaatcaattc aagagtctca ttgctgcttc cagaatttct tctgaagtaa
     3421 catgtcgatc cgctgctaca taaatgactt tatgatctcc ggtcagagat ggaaaccctg
     3481 cagccattac agtgaggtgt gttgtttcgc catttggata ttcacgcatg atggtgttaa
     3541 ctccggtcat cactggcact accattgctg gttcagagtt aaaaaaaact attatttttt
     3601 tcataatgtt accgtaatat gtgagtatcc atcgactaga cactaagcaa aaaagctccc
     3661 gaaggagcct tcattttcac tttcttaaat ctaacgacag acggctaaca tttaagtatt
     3721 gtgaaatatt atcaaatgta atcatcattg atttacaaaa gatacatttt gccccgaaag
     3781 gattcctgtc agaaacatca aaagatgatg ttctatactg agaaccatgg caacacgggc
     3841 atctaaagtg aatatggttt gtaatatttt ctacctcaaa gtgccactac atgaacagcc
     3901 gcggggcctt taggtccgtt ctcaatacca aattcgactt cctgattctc agttaacgtt
     3961 ttgaaatcgt tgctctgaat agctgagaaa tggacaaaca catctttgct accatctttc
     4021 ggcgtgatga aaccaaaacc tttttcaggg ttaaaccatt tcactaaacc agtcattttg
     4081 ttagacatca ttattacctt ttgaagaagt tagcccttgg gcagaatggt ccgaaaaaaa
     4141 atatcagaga gaaaaaccaa caaggaaatc tcaagaggta caaataataa aattataaca
     4201 atgactgctt cagataattt tgtaacaaac cagaacacca ttaacgcatg attaaccaac
     4261 catagcaagg attacttttg taaagaaaaa cacacgaatg aaacaatagc tttatttatt
     4321 aataaaacgt gtcattctgt ctaatgacct tttatcttac ccttaagatt tcaagaattt
     4381 tgactcatgg aagagtcct
//
LOCUS       DAZKIV010000242         4394 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZKIV010000242 DAZKIV010000000
VERSION     DAZKIV010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.244"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..357
                     /locus_tag="ACHOT6_004577"
     CDS             76..357
                     /locus_tag="ACHOT6_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HFP9949663.1"
                     /translation="MNTLFLLMAEFNTSNIELSAVSQKYFGMSPATAEAKANACKLPV
                     PTYRIGTSQKAKRCINIQDLAEYIDKRREEGRIEWERVRTDKQKSKEHH"
     gene            complement(549..1583)
                     /locus_tag="ACHOT6_004578"
     CDS             complement(549..1583)
                     /locus_tag="ACHOT6_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001128527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA family protein"
                     /protein_id="HFP9949664.1"
                     /translation="MPLKASLNGKEIHSFEFNSQEWEYLKQTYKSQSLFMSCCGQPGI
                     PKTSKLNNYYFAHKSKSDCLYAKESAEHLYLKFLIAKLASDAGWSVTTEKQGVTPTGE
                     QWIADVFCTRNNAKLVFEVQLSPQKDDEFKERQKRYIASGVRALWLRKLRKGSKEHGG
                     NIYHSYDLPVFGVRQNENGDLYLPQFGISVREFIAGVFARKLLWFPKVGDVLTAKIES
                     RVTKCWRCKKRTGIIRGISVYSKSVFVKFLSFRDSDVKELIATHVDNKKLWAAGIGTV
                     KDRDSDCYLSNCCIHCYSLIGDFFLLEVFDYPNDKQKIMIEFTFTYASNMKILSSEWV
                     FDGKKAEMFF"
     gene            complement(1722..1895)
                     /locus_tag="ACHOT6_004579"
     CDS             complement(1722..1895)
                     /locus_tag="ACHOT6_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949665.1"
                     /translation="MEDQKATKPQLKFDTMKAFAGMGAAVEVLMKAAPNAFTHVTVSG
                     KEQQGKLRRRKAV"
     gene            complement(1935..2321)
                     /locus_tag="ACHOT6_004580"
     CDS             complement(1935..2321)
                     /locus_tag="ACHOT6_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949666.1"
                     /translation="MLAPTGPCNHLHVICNDPVYYPVNDCYCVLVVNISSIKDGVPHD
                     PSCVLNSGDHRFIKHPSYVVYAEAIIWRVDNMVRKQRSGEISVHDDMPEATFNRILDG
                     FDISDEVTPKNLKFKNKYCVSSIDDE"
     gene            complement(2351..2923)
                     /locus_tag="ACHOT6_004581"
     CDS             complement(2351..2923)
                     /locus_tag="ACHOT6_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Panacea domain-containing protein"
                     /protein_id="HFP9949667.1"
                     /translation="MFCEEKVAQMAAYLLLKRGGRMAYLKLMKLLYLSNRKSILKHGR
                     MIGEDSLYSMKFGPVMSNTLNLIRGKAEGIGDYWYNLIETNGHDVLLRSDPREMDADE
                     VFDELSRADIRILDEIYSLYGHMNRFDLANMTHLESVCPEWHNPGNSRKPIDLKEMLI
                     SEGKSEDEANRIIGKMEESQKLKEFSLQLS"
     gene            complement(3176..3280)
                     /locus_tag="ACHOT6_004582"
                     /pseudo
     CDS             complement(3176..3280)
                     /locus_tag="ACHOT6_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
     gene            complement(3643..4389)
                     /gene="yjbM"
                     /locus_tag="ACHOT6_004583"
                     /pseudo
     CDS             complement(3643..4389)
                     /gene="yjbM"
                     /locus_tag="ACHOT6_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         1355 a          960 c          762 g         1317 t
ORIGIN      
        1 ttgctgtact tcagagcaaa gacgctatta attaatatat tactcccggg tgcagccggg
       61 ataatggaga aatttatgaa caccttgttt ttactgatgg ctgaattcaa tacctcaaac
      121 attgaactct cagcagttag ccaaaagtac tttggtatga gtccagccac agcagaagca
      181 aaagcaaacg cttgtaagtt gcctgttcct acatatcgca ttggcacatc acaaaaagca
      241 aaacgttgca tcaatattca ggatcttgcg gaatacatag ataaaagacg agaagaagga
      301 cgtatcgagt gggaacgggt cagaacagac aaacagaaga gcaaagaaca tcactaaaga
      361 aaaaaaacgc ataaaggcca atgccgttca cttaagatga gctgctctgc atttcaccag
      421 ataatgttat tttgatatct taatttccct cggctctgat agtcggggca ttttgttatg
      481 aacacaatac ccagaacatc ccccagatgt tcctgtcatt tcgggatggc aacgaggata
      541 agacagcatc agaaaaacat ttcagctttt ttaccatcaa atacccactc gctggataag
      601 attttcatat tgctagcata agtaaaagta aattctatca ttatcttttg tttatcattc
      661 gggtagtcga aaacctctaa taaaaagaaa tctcctataa gcgaataaca atgtatgcaa
      721 cagttagata aataacaatc actgtctctg tccttaacag taccaattcc agccgcccag
      781 agttttttgt tatccacgtg agtggctatt agttctttga catcagaatc acgaaaagat
      841 aaaaacttga cgaatacgga cttactgtaa acagaaatac ccctgataat tccagttcgt
      901 ttcttacatc tccagcattt tgtaactcgc gattctattt ttgcagtcag cacatcccca
      961 acctttggga accataatag ctttctggca aaaacacccg cgataaactc cctgacacta
     1021 atcccaaact gtggtagata caagtcccca ttttcattct gacgcacacc aaaaacaggt
     1081 aaatcgtagc tatggtatat attcccacca tgctctttac ttcctttacg taactttcgc
     1141 aaccacagtg cacggactcc tgatgcgata tatcgttttt gacgttcttt aaattcatcg
     1201 tctttttgcg gtgataactg tacttcgaaa accagtttcg cgttatttct ggtacaaaac
     1261 acatcggcaa tccattgttc tcctgttggg gtcacccctt gtttttcggt agtaactgac
     1321 cagccagcat cactggctaa ttttgcgatt aagaatttga gatataaatg ttctgcgctt
     1381 tctttcgcat acaggcaatc gcttttggat ttatgtgcaa agtaatagtt attaagtttg
     1441 cttgttttag gtataccagg ctgaccacag cacgacataa acaaagactg gcttttatat
     1501 gtctgtttaa gatattccca ttcctgagag ttaaattcaa aagaatgaat ttcttttccg
     1561 ttaagtgacg cttttagtgg cattcatgct acctgtattt accctcctgg cgagggaaat
     1621 taagatatca aaaaaccatt atctggtgaa acgcagtacc accatactta agtgaacgac
     1681 attatgtcta aagacatttt taaaaaatca ccacctatga atcatactgc tttgcgacga
     1741 cgaagcttac cctgctgctc tttaccagag acagtaacgt gagtgaacgc attaggagca
     1801 gccttcatca gaacttcaac agcagcaccc atacctgcga atgctttcat tgtgtcgaac
     1861 ttaagctgtg gcttggttgc tttttgatct tccatagaaa acaccagaag ctataccgaa
     1921 acaattcctg ttgtttactc atcatcaata gatgatacgc aatatttatt tttaaattta
     1981 aggttctttg gcgtaacttc atcagatata tcaaaaccgt ccagaattct attgaatgta
     2041 gcttctggca tatcatcatg aacagaaatc tcacccgatc gctgctttct aaccatgtta
     2101 tccactcgcc aaattatagc ttcagcgtaa acaacataac ttggatgctt gataaagcga
     2161 tgatcaccgg aattcaagac gcaagacgga tcgtggggta ccccatcctt gatactagaa
     2221 atattaacaa ctaaaacaca ataacaatcg ttaacggggt aataaacagg atcattacaa
     2281 atcacatgaa gatgattgca tggcccagtt ggggcaagca cagttccttt cctgtatggc
     2341 tgataatccg tcatgataat tgcaaagaaa attccttaag tttctgagat tcttccattt
     2401 tgccaattat gcgattagcc tcatcctcgc ttttaccctc actgatcagc atttctttca
     2461 ggtctatagg cttacgagaa ttgccaggat tgtgccactc tggacaaacg ctttctaaat
     2521 gcgtcatgtt tgcgagatca aatcggttca tatgcccata cagagaatag atttcatcta
     2581 aaatccggat atctgcacgg ctcaactcat caaagacctc gtctgcatcc atttccctcg
     2641 gatctgaacg caacaacaca tcatgcccgt tcgtctctat caagttgtac cagtagtcac
     2701 caatgccttc agccttacca cgaatcaagt tcagcgtatt cgacatgact ggaccaaatt
     2761 tcatagagta aaggctatct tcgccgatca tcctgccatg cttcaaaatc gacttgcggt
     2821 tagacagata gagcagcttc atcaatttca gatatgccat gcgcccacct cgcttaagaa
     2881 gtaggtatgc agccatttga gctacttttt cttcgcaaaa catataagaa acctcaaaga
     2941 tagtggactt tgtaattatt ctcgatactt catgggttac ttcatcacca acatggcttc
     3001 ttatggtgtg cccacttggg cctaagcaac cagttacctt gtttgtcaag tttatagctg
     3061 gctttaatca tggtcaattg aagtgatgag caaatgatgc tttctaagaa taacaggttc
     3121 ccaataggct cccacaaagt gtataaccac ttgtttttca aaaacggtac atcctatcga
     3181 gcattggtgc aacgctaaac cgaccactcc agtgaacgtc agttttttca ggcattgcgc
     3241 tggtttggtt gattttttgc atttcagaat taccgtgcat tttcaaatgt agagattatt
     3301 ttatcaatat atcatggggt tatgttattc agcttcactg ttcaggaggc tcaatagcgg
     3361 ggtactatac cataacaaca ggaagcgcct gtctcattgc aaaagaaaat tgagatcctc
     3421 tcaaggcatg aagctctcac gaagtgatgg aaataatctt attagccgtt aactttgtta
     3481 aggccaatga taaacaatcc aggttcgacg ataaataaaa aatcacacat taaactctgg
     3541 tgatatatct ccctgctaat agcattgata gagaaaaaag aacccaataa atattgggtc
     3601 cttttatata atgccttcca tactatcgaa gaacttcaca tattatttct ccgatttaac
     3661 cccgaacaaa tcataaatta atttagaagt gtctgtaagt attttaatct cttcctttga
     3721 ggttgggtca aacgacttcg cgaagctaat taattgtggt gcagcatctc gcattttgct
     3781 taaaatatca ggatcgagcg ttccttcatt caccagatgt gacatcttat ccagatagcc
     3841 atcaaaattc atttctcccc catcagccag acgcttcatc tctcccaggt acttcttcat
     3901 atcacgttga gataattttt caaactgagc tttcaggtag ctctcattat tttcattaat
     3961 atacactgtt tctgataaat ctgcgtaggc actagaatat gaaagtgaaa ttaacagcga
     4021 agcaaataat tttaagctgt tgtcatcatg ttcctggcag gcattaacaa acgttaaaaa
     4081 accagaacct attgcttgaa aatgtatatt cgctaaaggt tcattatcct tagattcctc
     4141 ataaaatagc tgaaccgtgg agggaaggcc attcttagtt tctacatcaa aagtacattg
     4201 ttcaatggga gtcgcatctt tattatcccc aggattacat atactattaa tcgcgaaatg
     4261 aaaaatagcc cggtcaacaa ctgatgcaat aaagtcacga tcgtttaaat cctcatcagt
     4321 gcaatcgtca atatacttat tgacccacat tttgagatca tttatttgct caattaaaaa
     4381 cacatttttt tacg
//
LOCUS       DAZKIV010000243         4231 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZKIV010000243 DAZKIV010000000
VERSION     DAZKIV010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.245"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHOT6_004584"
     CDS             complement(<1..209)
                     /locus_tag="ACHOT6_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFP9949668.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHOT6_004585"
     tRNA            complement(324..399)
                     /locus_tag="ACHOT6_004585"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHOT6_004586"
     tRNA            complement(406..480)
                     /locus_tag="ACHOT6_004586"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHOT6_004587"
     tRNA            complement(597..681)
                     /locus_tag="ACHOT6_004587"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHOT6_004588"
     tRNA            complement(690..765)
                     /locus_tag="ACHOT6_004588"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOT6_004589"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHOT6_004589"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFP9949669.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOT6_004590"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHOT6_004590"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFP9949670.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOT6_004591"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHOT6_004591"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFP9949671.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1092 a         1050 c          945 g         1144 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 aaatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcttgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat g
//
LOCUS       DAZKIV010000244         4149 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZKIV010000244 DAZKIV010000000
VERSION     DAZKIV010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.246"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..783
                     /locus_tag="ACHOT6_004592"
     CDS             <1..783
                     /locus_tag="ACHOT6_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9949672.1"
                     /translation="KEDNGNISQTAKSPAERKRAQRERERKREQNGDCHGESRNVTHM
                     SRRVTTDKDTDKDTDQEDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALK
                     ADSGAVQQVMTARQEQSHQLQQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVW
                     REYPLRAGANPKKSAFSAWKARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRAT
                     TFFGPDRNFENPWLLPVSGTNNQRCVNHISEPDNEIPPGFRG"
     gene            824..1246
                     /locus_tag="ACHOT6_004593"
     CDS             824..1246
                     /locus_tag="ACHOT6_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFP9949673.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNTRKKLSRAKLKKPAVVDPDLIWSLPDG
                     EIRRYDRHQNIICRESRRSEVMQRVLAFYRGNFQEVME"
     gene            1243..1539
                     /locus_tag="ACHOT6_004594"
     CDS             1243..1539
                     /locus_tag="ACHOT6_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HFP9949674.1"
                     /translation="MRVRVYIAGPMTGYENFNREAFHKVEEELKREGHTVLNPAVLPD
                     GLTQPHYMDICMAMIRCVDAIYMLNGWQRSAGAKAELALAEKLGHAVIYQEVAQ"
     gene            1536..1997
                     /locus_tag="ACHOT6_004595"
     CDS             1536..1997
                     /locus_tag="ACHOT6_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB
                     domain-containing protein"
                     /protein_id="HFP9949675.1"
                     /translation="MREVNYEALREAAQNYQSTLAWYQATPDSPNAERDCDAALAAFK
                     RHIRHREADIIAGLLDGLEEAKSQLNEQREYYEGVISDGSKRIAELDAREVQLPTRYD
                     LRYGHPINADERHVMIPKENGSWLYLIDLEHALRVAGIRIKGEEHGNKTRG"
     gene            1975..2331
                     /locus_tag="ACHOT6_004596"
     CDS             1975..2331
                     /locus_tag="ACHOT6_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949676.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVKLLRQEEQERRKNIEKLIQIE
                     RNAKARIRRVTKSRTE"
     gene            2382..2594
                     /locus_tag="ACHOT6_004597"
     CDS             2382..2594
                     /locus_tag="ACHOT6_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949677.1"
                     /translation="MLNTQKAINAEKYNEWARKFSEQIFKITGDENAAKNELEPWTPE
                     GADPNYCWREVDPVDAANEAMSYHND"
     gene            2627..2844
                     /locus_tag="ACHOT6_004598"
                     /pseudo
     CDS             2627..2844
                     /locus_tag="ACHOT6_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
     gene            2846..3109
                     /locus_tag="ACHOT6_004599"
     CDS             2846..3109
                     /locus_tag="ACHOT6_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949678.1"
                     /translation="MATLTKKERAWLNELQEVLDRCPSPKKIGFYTIGDKSIYLYDLR
                     RMDEIMEALDNRSSMDWCVAVHDMNAGFDEKILFPSSVESTAG"
     gene            3120..3245
                     /locus_tag="ACHOT6_004600"
                     /pseudo
     CDS             3120..3245
                     /locus_tag="ACHOT6_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3801..3989
                     /locus_tag="ACHOT6_004601"
     CDS             3801..3989
                     /locus_tag="ACHOT6_004601"
                     /inference="COORDINATES: protein motif:HMM:NF016343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFP9949679.1"
                     /translation="MPDTKTAVLVARDFGRKGDWRMKWATYIPGHPDANDGWIIPGAS
                     WIPSHWMPLPEPPQEVRQ"
BASE COUNT         1208 a          850 c         1163 g          928 t
ORIGIN      
        1 aaagaggaca acggcaatat ttcgcaaacc gcaaaatctc cggcagagcg caagagggcg
       61 cagcgagaga gggaaagaaa gcgggaacaa aatggcgatt gtcacggcga gtcacgaaat
      121 gtcacgcaca tgtcacgacg agtcacgaca gataaagata cagataaaga tacagatcaa
      181 gaagatcaaa acactatggt ccatggcgta aaaaacgcca cgaaccaggc aggggatgtt
      241 cagaccgtca atcctggtca gccagcaggc acgacaccgg aagccgattc agcgtatgcg
      301 ctgaaagccg attcgggcgc tgtgcagcag gtgatgaccg caaggcagga gcaatcacac
      361 caacttcagc agcctgaagc cgattccgcc attcagcggg aagccgatcg ggtagtcccg
      421 gaaaacaccg gtcagtctgt gggacgagtg gattatccgg atgtgttcga acaggtctgg
      481 cgggagtacc cgttgcgtgc tggggcaaac ccgaagaaat ccgctttcag tgcctggaag
      541 gccagattac gcgagggggt gccaccagag gccatgctgg atggcgtgag gcgttacgca
      601 agatacttgg cggctaccgg gaaaacggga acggaatttg ttcagcgagc gacgacgttt
      661 tttggaccgg accggaattt tgagaacccc tggttgctcc cggtaagcgg cacgaacaac
      721 cagcgttgtg tgaatcatat ttctgaaccg gataacgaaa ttccgccggg cttcaggggg
      781 taagtgttaa tttctggtca tgaggtaatt ttcaggaggg cttgtggcaa aagtttttac
      841 acaagaagag cgggaaaaaa ttaaagggca ggttcttgaa ctcgtacgcc agagtgggcg
      901 cgagacgtta cgacaactgg aagctaaaac tggggcaaca agatatctga tgagcgttct
      961 ggccagagag ctggttgcca gtggcgatgt atacaactct ggttacgggt tattcccgtc
     1021 tgaacaggcg cgtaaggact ggcaaaatac ccgtaaaaag ctctcaaggg caaagctgaa
     1081 gaaacctgca gtggttgatc cggaccttat ctggtcgtta ccagacggcg aaatacgccg
     1141 ctacgacagg caccagaaca taatttgtcg tgagagcagg aggagcgaag ttatgcagcg
     1201 tgtgctggcg ttctatcggg gaaactttca ggaggtgatg gagtgagggt gagagtttat
     1261 attgccggtc caatgacggg atatgaaaat ttcaaccgtg aggcatttca caaggtggaa
     1321 gaggaactga aacgggaagg gcataccgtc ttaaacccgg cagtacttcc ggacgggctg
     1381 acacagccgc actacatgga tatttgcatg gcaatgattc gttgtgtgga tgcgatttac
     1441 atgctgaatg gctggcagcg gtcagcgggc gctaaggcag agctggcact ggcggagaaa
     1501 ctggggcatg cggtgattta tcaggaggtg gctcaatgag agaggttaac tatgaggcgc
     1561 ttcgtgaggc agcacaaaac tatcagtcga cgctggcgtg gtatcaggct accccggaca
     1621 gcccaaatgc tgaacgggat tgtgatgcgg ctcttgctgc gtttaagcgt catatccgtc
     1681 atcgggaagc ggatattatc gctggtttgc tggatggact ggaagaagca aaatcacaac
     1741 tcaacgagca gcgtgagtat tacgaaggcg ttatctctga tgggagcaag cgtattgctg
     1801 aactggatgc gcgggaagtt caattaccga ctcgctacga ccttcgatat ggacacccga
     1861 taaatgcaga tgagcgacat gtcatgatac ctaaagaaaa tggcagttgg ctttacctga
     1921 ttgacctaga acacgcatta cgcgtcgctg gcattcgcat caaaggagag gagcatggaa
     1981 ataaaaccag aggatgagtt aagcaatatc gttttatttc cggtaaaaga ggatgaccct
     2041 cgtaatcagg ttaattttct ttatgagcca tcggaaagac catattgtca tcacgcctct
     2101 gtccgggttg acgaaaaaga gcgtcaggtc cgctgtaaaa tctgcggtgc agttgtggag
     2161 ccatttgact ggatgctctc tgtggcgaaa agagaaacta ggctggcaga tgatgtaaag
     2221 ctattgcgcc aggaggaaca ggaaaggcgg aaaaatatag aaaagttaat tcagattgag
     2281 cgtaacgcga aagcgcggat acgcagggtg acaaaatcca gaactgaata aataaattta
     2341 gcgctgtaaa taaaatctaa tccttaactg gaggtgtatt tatgttaaat acacagaaag
     2401 ccattaatgc ggaaaaatat aacgagtggg caagaaaatt ctctgagcag atttttaaaa
     2461 ttactggcga tgagaatgcg gcaaaaaatg aattagaacc gtggacgcct gaaggagccg
     2521 acccaaatta ttgctggagg gaggttgatc cagttgatgc tgcaaatgaa gctatgagtt
     2581 atcacaacga ttaatgtcag gaggccgccc gaaagggcgg taatgaatgg tcacattatt
     2641 tagaaaaaat atccgcgaaa gagtagaaca acagaatttc tgtttctcat tctgtttatc
     2701 gtgttgatga taccgatatc cccgttaatc ctagtctgga taatcggaaa aataattgag
     2761 ccagttattg aattgtataa cgacgtggta tgggcgtcat tcaacacact gcacaataaa
     2821 attaatccgt ataaggaaaa ctgatatggc aactttgaca aaaaaagaac gggcatggtt
     2881 gaacgaatta caggaagttc ttgatcgctg tccatcaccg aaaaaaattg gcttttacac
     2941 cattggcgat aaaagcattt acctgtatga cctacgccgc atggatgaaa tcatggaggc
     3001 tcttgataat cgttcgtcga tggattggtg tgttgctgtt catgatatga atgcagggtt
     3061 tgatgaaaag attttgttcc cctcatcagt tgaaagcact gcgggttaag gagtaacaca
     3121 tgaccactat taccaaagaa cgtattgaat tgttcattaa aaatccgctt gaaaacgggc
     3181 ttacccgtgg tgaacaaatg gaactggcac ggattgcgct ggcatcgctg gaagcagagc
     3241 cggtggcaaa gattatagct cattacccat taggggttga cgtaggcaaa caaaaatttg
     3301 tacaggccat tagagagctt cctgactttg gcggatatct atttgccgcc cctccagcgc
     3361 cgatagtgcc ggaagaaatg tattggcagg atgcgccagt tgaaggcagc agcaaagcgg
     3421 ctgcatacgc tacaggctgg aacgattgcc gcgaagccat gtttcagtcc ggaaactttc
     3481 gggaaaataa agattcgtca accaataatt ttcggaaaat cccggaagcg tcaaccagct
     3541 ctccggtaac tccggctctt ctgcctggtg gtttcaccat tgaggaggcg aaggaattac
     3601 atgaagacct ggcacgcagc cacataagca aggccttaag tggcgaaaag atgaaaaaga
     3661 aagatcgcga tgctgatttg cgctggattc atggcgttat agttcaggca gcgtggtttg
     3721 taaaagcatc actggagcag aatgcactat cgggcaacta tccggtaact ccggatagtt
     3781 ggataagctg tagtgagcga atgccggata ccaaaacagc cgttcttgtt gccagggatt
     3841 ttggcaggaa aggtgactgg cgaatgaaat gggcgactta catcccgggg catcctgacg
     3901 ctaatgatgg gtggataata cctggtgcgt cgtggatacc atcacactgg atgcctctac
     3961 cagaaccgcc gcaggaggtg cgccaatgaa ctggcctgaa gcatttgcaa ttacaggcgt
     4021 tgctatggct atcgcttttt tagtatatgt tatttgtcgg tgagggtaaa aacgttcgcc
     4081 gggattaaca ccaaaggagg gaatatgtcg gatgatatat cactggcaat ggaaggtgcg
     4141 ctggctgtt
//
LOCUS       DAZKIV010000245         4136 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZKIV010000245 DAZKIV010000000
VERSION     DAZKIV010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.247"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2052
                     /locus_tag="ACHOT6_004602"
     CDS             <1..2052
                     /locus_tag="ACHOT6_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFP9949680.1"
                     /translation="RSNHNAGKSNYAYLNLQSGINIGSWRLRDNSTWSYNSGSSNSSD
                     SNKWQHINTSAERDIIPLRSRLTVGDSYTDGDIFDSVNFRGLKINSTEAMLPDSQHGF
                     APVIHGIARGTAQVSVKQNGYDVYQTTVPPGPFTIDDINSAANGGDLQVTIKEADGSI
                     QTLYVPYSSVPVLQRAGYTRYALAMGEYRSGNNLQSSPKFIQGSLMHGLEGNWTPYGG
                     MQIAEDYQAFNLGIGKDLGLFGAFSFDITQANTTLADGTRHSGQSVKSVYSKSFYQTG
                     TNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVKPPTGDTNEQTQFIDYFNLFYNKRGQE
                     QISISQQLGNYGTTFFSASRQSYWNTSRSDQQISFGLNVPFGDITTSLNYSYSNNIWQ
                     NDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNASYSMSNDLKGGMTNLSGVYGTLLPDN
                     NLNYSVQVGNTHRGNTSSGTSGYSSLNYRGAYGNTNVGYSRSGDSSQIYYGMSGGIIA
                     HADGITFGQPLGDTMVLVKAPGADNVKIENQTGIHTDWRGYAILPFATEYRENRVALN
                     ANSLADNVELNETVVTVIPTHGAIARATFNAQIGGKVLMTLKYGNKSVPFGAIVTHGE
                     NKNGSIVAENGQVYLTGLPQSGQLQVSWGKDKNSNCIVEYKLPEVSPGTLLNQQTAIC
                     R"
     gene            2066..2596
                     /locus_tag="ACHOT6_004603"
     CDS             2066..2596
                     /locus_tag="ACHOT6_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949681.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            2609..3112
                     /locus_tag="ACHOT6_004604"
     CDS             2609..3112
                     /locus_tag="ACHOT6_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949682.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
     gene            3171..4085
                     /locus_tag="ACHOT6_004605"
     CDS             3171..4085
                     /locus_tag="ACHOT6_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9949683.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
BASE COUNT         1184 a          884 c          952 g         1116 t
ORIGIN      
        1 cgtagtaacc ataatgcagg aaaatccaac tatgcatatt tgaatttaca gagtggcatc
       61 aacattggta gttggcgact gcgcgataac tcaacgtgga gttataacag tgggagtagc
      121 aattcatctg acagcaataa atggcagcat atcaatacgt cggctgaacg tgacattatt
      181 cccttacgct cacggttaac ggtaggtgat agttataccg atggcgatat ttttgatagt
      241 gtgaactttc gtggcctcaa aataaattca acagaagcga tgttgcccga tagccaacat
      301 ggtttcgctc cggtgattca tggtattgcc cgtggaaccg cacaagtgag tgtaaaacag
      361 aacggatacg atgtttatca gactactgtt ccacccggcc cttttactat tgatgatatc
      421 aactctgcgg ccaatggtgg tgatctgcaa gtaaccataa aagaggcaga cggcagtatt
      481 cagacattat atgttcctta ttcgtctgtt ccggttctcc aacgtgctgg atatacgcgt
      541 tatgcgcttg ccatggggga atatcgtagt ggaaataacc tgcaaagctc tcccaagttc
      601 atacaaggta gcttgatgca tggtctggaa ggaaactgga caccttatgg cggaatgcaa
      661 attgcagaag attatcaggc cttcaacctt ggtattggga aagatttagg actttttggt
      721 gccttttctt tcgatatcac gcaggccaat acgacacttg cagatggcac ccgtcacagt
      781 gggcaatcgg ttaaatccgt ctacagcaaa tccttctacc agacaggaac caatatccag
      841 gtcgcaggat atcgctattc tacgcaaggt ttttataact tatccgacag tgcctacagt
      901 cgaatgagtg gttacaccgt caagcctcct accggagaca ccaatgagca gacacaattt
      961 attgattatt ttaatctgtt ctacaataag cgtggtcagg aacaaataag catctctcag
     1021 cagcttggaa attacggtac gacatttttc agtgccagtc gccaaagtta ctggaacacg
     1081 tcacgcagcg accagcaaat atcatttgga ttaaatgtgc cgtttggtga tattacgact
     1141 tcgctgaatt acagctattc caataatata tggcaaaacg atcgggatca tttactcgct
     1201 tttacgctta atgttccctt cagtcattgg atgcgtacag acagtcagtc ggcatttcgt
     1261 aattcaaacg ccagttacag tatgtcaaac gatttgaaag gcggcatgac caatctatcg
     1321 ggggtttatg gcactctgct gccggataat aacctgaatt atagcgttca ggtcggtaac
     1381 acccacagag gtaatacatc gtctggcacc agtggttaca gttctcttaa ttatcgtgga
     1441 gcttatggta atactaatgt cggttacagt cggagtggtg acagcagcca gatttattac
     1501 ggaatgagtg gtgggattat tgctcatgct gatggcatca cctttggaca gccgctgggc
     1561 gacacaatgg ttctggttaa ggctcctggt gctgataatg tcaaaataga gaaccagacc
     1621 ggaattcata ccgactggcg tggctatgcc atattaccat ttgcgacaga atatagagaa
     1681 aaccgtgttg ctcttaacgc gaattccctt gcagataatg ttgaactgaa tgaaaccgtg
     1741 gtcactgtca tcccaactca cggtgctatt gccagagcaa catttaatgc acaaatcggc
     1801 gggaaagtat taatgacgtt gaagtacggt aataagagcg ttccattcgg tgcaattgtc
     1861 acacacggag agaataaaaa tggcagcatt gtcgcggaaa atggtcaggt ttatctgact
     1921 ggacttccac agtcagggca attacaggtt tcatggggca aagataaaaa ctcaaactgt
     1981 attgtcgagt acaagcttcc tgaagtttct cctggtacct tactgaacca gcagacagca
     2041 atctgtcgct aaggaacaat agattatgaa atacaataac attattttcc tcggtttatg
     2101 tctggggtta accacctatt ctgctttatc cgcagatagc gttattaaaa ttagcgggcg
     2161 cgtcctcgat tatggctgca cagtctcatc ggattcgctt aattttaccg tagatctcca
     2221 aaaaaacagt gccagacaat ttccaacgac cggtagcaca agtccagccg tcccttttca
     2281 gattacgtta agtgaatgca gcaaagggac aacgggggtt cgggttgcat ttaacggtat
     2341 tgaggatgca gaaaataata ctttgttgaa actggatgaa ggaagcaata cggcctccgg
     2401 tttgggtata gaaatattgg acgcaaatat gcgtccggtg aaactgaatg accttcatgc
     2461 cgggatgcag tggatcccac tggtaccaga acagaacaat attttgcctt actccgctcg
     2521 tctgaagtca actcagaagt ccgtcaatcc gggactggtg agggcttcgg caacctttac
     2581 ccttgaattt caataatcag agtcagatat gaaaagactt cacaagaggt tcctgttagc
     2641 tacgttttgc gcgttattca cagcaactct ccaggccgcc gatgtcacta tcactgttaa
     2701 tggtcgggta gtcgctaaac cctgcactat tcaaaccaaa gaagctaacg ttaatctcgg
     2761 ggatctttat acgcgcaatc tgcaacaacc tggttctgca tctggctggc acaatattac
     2821 tttgtcatta accgattgtc cggttgaaac aagtgcagtg acggcaatcg tgacaggttc
     2881 aactgacaat acgggttatt acaaaaatga aggtactgcc gaaaatattc agatagagct
     2941 gagggatgac caggatgctg cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga
     3001 gatcactcgt aatgcacagt ttccacttaa ggcaagagct atcacggtga atggaaacgc
     3061 aagccaggga acgatcgagg cgctaatcaa tgtgatctac acctggcaat aacgtcgatt
     3121 cgattctctt agccttcttt ttaaatcaat ttttaacctt aggatatatc atgggtaaaa
     3181 caatcagtat aaaggtcctg ttcggtattt atcttctatt aatggcaggc aaagtttttg
     3241 ccttcagttg taatgtcgat ggcgggtcaa gtattggagc aggtacaacc tctgtttatg
     3301 ttaatctcga ccctgtaata cagccgggcc agaatctggt tgtagacttg tctcagcata
     3361 taagttgctg gaatgattac ggcggctggt acgacactga tcatataaac ctggtacaag
     3421 gctcagcttt cgcaggctcc ctacagtcat ataaaggatc gctgtactgg aataatgtga
     3481 cctacccctt cccgttgacg acaaatacca acgtattgga tattggtgat aagacaccga
     3541 tgccattacc gttaaaactc tatattaccc cagttggggc ggccggtgga gttgtcataa
     3601 aagcggggga agtcattgct cgtattcata tgtataaaat tgcaacatta ggaagcggaa
     3661 atccgcgtaa ttttacgtgg aatattattt caaataacaa tgttgttatg ccaacaggcg
     3721 gctgcaccgt tgatagtcgt aatgtcactg tggatctccc ggactttcct ggaagcgcag
     3781 aaatacccct tggcgtatat tgctcaagcg aacaaaagct ctcattttat ctttcgggtg
     3841 ccaccactga cagtagccga caagtgtttg ctaacacagc acctgatgca acaaaagcat
     3901 cgggagtggg tgtctctctg atgcgtaatg gaaaaatact ggctacggga gaaaatgtct
     3961 ccctggggac agtaaataaa agcaaagtac ctctgggttt aagcgcaacc tatggacaaa
     4021 caggaaacaa ggttgcagcc ggaaccgtcc agtcagtcat tggtgtcact tttatctatg
     4081 aataattgtg attttgatct tagtctatgc gctccattca cgaaaattgc gcggat
//
LOCUS       DAZKIV010000246         4075 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZKIV010000246 DAZKIV010000000
VERSION     DAZKIV010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.248"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            156..764
                     /locus_tag="ACHOT6_004606"
     CDS             156..764
                     /locus_tag="ACHOT6_004606"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFP9949684.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            862..2253
                     /gene="selA"
                     /locus_tag="ACHOT6_004607"
     CDS             862..2253
                     /gene="selA"
                     /locus_tag="ACHOT6_004607"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFP9949685.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2250..>4075
                     /gene="selB"
                     /locus_tag="ACHOT6_004608"
     CDS             2250..>4075
                     /gene="selB"
                     /locus_tag="ACHOT6_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFP9949686.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDA"
BASE COUNT          913 a         1033 c         1210 g          919 t
ORIGIN      
        1 agtgagatat ccatcagata gaaacaatta ggaaaatatt tatatcacca tgataaatat
       61 gtgatttatt tggaagtaac aaaccatcaa cattttctgc cctttttccc atcaccaggc
      121 catactgata tttttcctcg cacaaaggac tcggcatgaa actcgtcggt agctacacca
      181 gcccgtttgt acgcaaactt tctattctgt tgttagaaaa gggcataact ttcgaattta
      241 ttaatgaact gccctataac gcggacaacg gcgtggcgca atttaacccg ttaggaaaag
      301 tgccggtgct ggtgaccgaa gagggcgaat gttggtttga ttcgccgatc atcgctgaat
      361 atattgaatt aatgaatgtc gctccggcga tgttgccgcg cgatccgctg gagtcgttgc
      421 gggtgcgcaa aattgaggca ctggcggatg gcattatgga tgccgggctg gtatcggtgc
      481 gtgaacaggc gcgtccagcg gcgcagcagt ctgaagatga attgttacgc cagcgggaga
      541 aaatcaaccg cagtctggat gtgctggaag gatatctggt cgatggcaca ctcaaaaccg
      601 atacggtcaa tctggcgact atcgccattg cctgtgccgt cggatatctc aatttccgcc
      661 gcgttgcgcc aggttggtgt gtcgaccgcc cacacttagt caaactggtc gaaaacctgt
      721 ttagccgcga aagttttgct cgcaccgaac cgccaaaggc ttgatgcgcg atatgtcctc
      781 ctgacccatc tcacgttaca atccgtggtt atgttaaacg cccttctccg tgtgagaggg
      841 ccttgatcag ccaggtttcc tatgacaacc gaaacgcgtt ccctctatag tcaacttccg
      901 gctattgatc gcttattgcg cgatagctcc ttcctttctt tgcgtgatac ttatggtcac
      961 acccgcgtgg tggaattgtt gcgtcagatg ctcgacgaag cgcgagaagt gattcgtggc
     1021 agccagacgc tgcctgcgtg gtgtgaaaac tgggcgcaag aagtcgatgc ccggttgacg
     1081 aaagaagcgc agagcgcgct gcgtccggtg atcaacctga cgggaaccgt gctgcatacc
     1141 aaccttgggc gagctttaca ggcggaagcc gcggtggaag ccgttgcgca ggctatgcgt
     1201 tcgccagtga ccctcgagta cgatctggac gacgccggac gcggacatcg cgatcgggcg
     1261 ctggcgcagc tgctgtgccg tattacgggg gcggaagatg cctgtatcgt caataacaat
     1321 gcggcggcgg tgttattgat gttggcggcc actgccagcg gaaaagaggt ggtggtatct
     1381 cgcggcgaac tggtggagat tggcggcgcg tttcgtattc ccgatgttat gcgtcaggca
     1441 ggctgcaccc tacacgaagt agggaccacc aaccgcacgc acgcgaatga ttatcgtcag
     1501 gcggtgaatg aaaataccgc actgttgatg aaagtacata ccagtaacta cagcattcag
     1561 gggttcacca aagcgataga tgaagcggaa ctggtggcgc tcggcaaaga gctggatgtt
     1621 cccgtagtga ctgatttagg cagtggctcg ctggtcgatc ttagccagta cggtttgccg
     1681 aaagagccaa tgccgcagga gttgattgcg gcgggcgtca gtctggtgag tttctccggc
     1741 gacaagttgt taggcgggcc gcaggcagga attattgttg gtaaaaaaga gatgatcgcc
     1801 cgcctgcaaa gccacccgct gaagcgtgca ttacgcgcgg ataaaatgac cctcgcggcg
     1861 ctggaagcca cgttgcgtct ttatttacac cctgaagctc tgagtgaaaa attaccgacc
     1921 ctgcgcctgc ttacccgcag cgcagaggtc attcaaatcc aggcacaacg tttacaggct
     1981 ccccttgccg cacattacgg cgcggagttt gcggtacagg ttatgccatg tctttcgcag
     2041 attggcagtg gttcgctgcc ggttgatcgc ctgccgagcg cggcattaac gtttacaccc
     2101 catgatggac gcggtagcca ccttgagtca ttagccgccc gctggcgtga attgccagtg
     2161 ccggtgattg gtcgtattta tgacggacga ttgtggctgg atttacgctg ccttgaagat
     2221 gagcaacggt ttttggagat gttgttgaaa tgattattgc gactgccgga cacgttgacc
     2281 acggcaaaac aaccttattg caggcgatta ctggcgtaaa tgctgaccgt ctgccggaag
     2341 aaaaaaagcg cggcatgacc atcgatctcg gctatgccta ctggccgcag ccggatggtc
     2401 gcgtgcctgg ttttatcgac gtacccggtc atgaaaagtt tctttccaac atgctggcgg
     2461 gcgttggtgg tatcgatcac gcgctgttgg tggtggcgtg cgatgacggc gtgatggcgc
     2521 agacccgtga gcatctggcg attttgcagc tgaccggtaa cccgatgctg acagtggcgc
     2581 tgaccaaagc cgatcgcgtg gacgaagcgc gtgttaatga ggttgaacgc caggtaaagg
     2641 aggtgctgcg ggaatacggt tttgctgagg caaaactgtt tatcaccgca gcaaccgaag
     2701 gtcggggaat tgatgccctg cgcgagcatc tgcttcagtt gccggaacgc gagcacgcca
     2761 gccaacatag tttccgcctc gctattgacc gcgcatttac cgtaaaaggt gccgggctgg
     2821 tcgtcaccgg tacggcgtta agcggggaag tgaaggtagg cgattcactc tggctgactg
     2881 gtgtaaataa accgatgcgt gtacgtgcgc tgcatgcgca aaaccagcca acagaaaccg
     2941 ccaatgccgg gcagcgtatc gcgcttaaca tcgcgggtga tgcggaaaaa gagcagatta
     3001 accgtggcga ctggctgctt gccgatgtgc cgccagagcc gttcacacgg gtgattgtcg
     3061 agcttcaaac ccatacaccg ctgacccagt ggcagccgtt gcatattcac cacgccgcca
     3121 gccacgtcac gggacgcgtt tcactgctgg aagataacct tgccgagctg gtgttcgaca
     3181 cgccgctgtg gctggcggat aacgatcgct tagtgctgcg cgatatctcg gcccgcaaca
     3241 cgctggcggg ggcgcgtgtg gtgatgctta acccgccgcg tcgcggtaaa cgtaagccgg
     3301 aatatctgca atggctggcg tctcttgcac gggcgcagag cgatgccgat gcgttatctg
     3361 ttcatctgga acgcggcgcg gttaaccttg cggatttcgc ctgggcgcgc cagctcaacg
     3421 gcgaagggat gcgcgaattg ctgcaacagc ctggctatat tcaggctggt tatagcttgt
     3481 tgaatgcgcc ggttgccgct cgctggcaac ggaaaattct cgacacatta gcgacttatc
     3541 atgagcaaca tcgcgatgaa cctgggcctg ggcgcgaacg tctgcgacgt atggcgttgc
     3601 caatggaaga tgaagcgcta gtactgttgc tgattgaaaa gatgcgcgaa agcggcgaca
     3661 tcctcagtca tcacggttgg ctgcatctgc cggatcacaa agcgggcttc agcgaagagc
     3721 agcaggccat ctggcaaaaa gcagagccgc tgtttggtga cgagccgtgg tgggtgcgtg
     3781 atctggcaaa agagacgggt accgacgagc aggcaatgcg cctgacgcta cgccaggcgg
     3841 cgcagcaagg aataattacc gcgatcgtca aagatcgtta ttaccgtaac gatcggattg
     3901 tcgaatttgc caatatgatc cgcgatctcg atcaggagtg tggttcaacc tgcgcggcgg
     3961 atttccgcga tcgcttaggc gtaggccgaa agctggcaat tcagattctg gaatattttg
     4021 accgcattgg ctttacgcgt cgtcgtggaa atgatcattt attgcgcgac gcatt
//
LOCUS       DAZKIV010000247         4073 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZKIV010000247 DAZKIV010000000
VERSION     DAZKIV010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.249"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(285..761)
                     /gene="racR"
                     /locus_tag="ACHOT6_004609"
                     /gene_synonym="rarC"
     CDS             complement(285..761)
                     /gene="racR"
                     /locus_tag="ACHOT6_004609"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFP9949687.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSCSDSKRELLDINSLYQAASD
                     EKRAIVAFLLSGNAAEPGWVDYDVRAYITAMEMKVSNYLKNQESERKSQNITKTGT"
     gene            886..1209
                     /locus_tag="ACHOT6_004610"
     CDS             886..1209
                     /locus_tag="ACHOT6_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949688.1"
                     /translation="MKKENYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRRLPA
                     ERCPAIERATKGGVLCEELRPDVDWSYLRRSSCYSQNMSMKQPNDKNDHTRSIKRQMI
                     HENQA"
     gene            1193..1618
                     /locus_tag="ACHOT6_004611"
     CDS             1193..1618
                     /locus_tag="ACHOT6_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9949689.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAEITRAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVAWMRSHSSDYFRE
                     LVDTHERLVRDADELVAVVIAGFNQMNRGGPAGNAVVAH"
     gene            1640..2605
                     /locus_tag="ACHOT6_004612"
     CDS             1640..2605
                     /locus_tag="ACHOT6_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949690.1"
                     /translation="MKLLFAERPLVINTQLAMKIGLNEAIVLQQLHYWLRDTGSGMEC
                     DGVRWIYNTTEQWLEQFPFWSESTLKRAFASLKTLGLLRCEKLNKSKRDMTNFYTINY
                     ESELLDGGKVSESIRSKCTAPSGQNDTMEEVKMARSIGSKRPNVIGSKWPDDLTENTT
                     EITTENKNTSRPEASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLACAQWIWGR
                     IVSLYEQAASDDGEISRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRVQRDPFWV
                     KNIMSPSKLREKWDELVIRLGRSPVQRCVNHISEPDTEIPPGFRG"
     gene            2612..3358
                     /locus_tag="ACHOT6_004613"
     CDS             2612..3358
                     /locus_tag="ACHOT6_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFP9949691.1"
                     /translation="MKNIATGGVLERIRKLTPQHVTAPCRTVDEWREWQLAEGRKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQAVIVVTVADVMSALHASYDDGQSGEKLLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASMRSVGMLTNLNYEAMKTLLGERI
                     MDRMTMNGGRWVSFNWESWRPNVVQPGITK"
     gene            3381..>4073
                     /locus_tag="ACHOT6_004614"
     CDS             3381..>4073
                     /locus_tag="ACHOT6_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFP9949692.1"
                     /translation="METVFDALKAMGKATSIELAARLDVSREEVLNELWELKKAGFVD
                     KSAYTWRVADNNVQQEQPAQAELPEETTTATVAKISECDLTATIEQRGPQTADELATL
                     FGTTSRKVASTLAMAISKGRLIRVNQNGKFRYCMPDDNLPAKPKAVSVTETDGKAFPQ
                     PAGVALPVQKDATQEDIKTETVADIVQSLPSFTATREDDLILPSLHMANRELRRAKNH
                     VQKWERVCAALRE"
BASE COUNT         1113 a          888 c         1099 g          973 t
ORIGIN      
        1 acaaaaacct gctgcatagt tattaaagtt gaaccctgcc gtcatgttct tacgcctcgg
       61 gctggctact taccccctga ccactgcctg gtaactcaaa gtattgccct gcattctgtg
      121 gggcggggtg ggttggtatg agaaaactat aggtaatgcc taattacttg tcaataggct
      181 atgcctaatg ctttttgcgc acaacctaat aggcgatggt atgtggaaga ggtaatggga
      241 gctaaataac gggatctagg tgcttcccgt cggaccatat aagtttaagt tcccgttttg
      301 gtgatgttct ggcttttccg ttctgattct tgatttttca gatagttact taccttcatt
      361 tccattgcag taatgtaggc gcgaacgtca taatcaaccc aaccaggctc cgcagcattt
      421 ccagataaga ggaaggctac aattgctctt ttttcatcag aagcggcttg ataaaggctg
      481 tttatgtcta aaagttctct ttttgaatct gagcaggaag gggttggtat ggggtattcg
      541 ttaagccccc aatgctctgg accaaccaca tcagaaaaga aacgccatag ttctggaagt
      601 ttatctttac ttatcgaacc tttcttaatc cagtcatgga ttgatggtgg ttggactttg
      661 aaatggcgtg cgatctccgc ctttgatttg acggctcctg atgcaatttt tttattaatg
      721 gcctgctcta tcgctcggcc taagtcttta ccactaagca ttgcttaata gtctcctatg
      781 tgcattgcat taggcaatcc ctacctcttg gttgttaggc atagcctatt gacaatgata
      841 ttaggcgccg cctaatatca ttgtgtgttt tttggagttc attcgatgaa aaaagagaac
      901 tattcattca aacgagcttg tgctgttgtt ggtgggcaat cagcaatggc cagactttta
      961 ggggtatcgc ctccaagcgt aaatcaatgg atcaaaggtg tacgtcggtt gcctgccgag
     1021 agatgtccag cgattgaacg agcaacaaaa ggtggagtcc tgtgtgaaga acttcgtcct
     1081 gatgttgact ggtcatactt acgacgttcg tcatgttatt cgcagaatat gtcgatgaag
     1141 caaccaaatg acaaaaacga tcatacccga agcatcaaga ggcaaatgat tcatgaaaat
     1201 caagcatgaa cacatccgca tggcgatgaa tgcctgggcg cgtccggacg gtgaaaaagt
     1261 tccggcagct gaaataaccc gggcttattt tgagctgggt atgacattcc cggaactgta
     1321 tgacgacagc catccggaag ccctggctcg caatactcag aaaattttcc gctgggtgga
     1381 gaaagacacc cctgatgcgg ttaaaaaaat tcaggctttg ttaccagcga tcgaaaaggc
     1441 aatgccacct ccgctggtgg cctggatgcg cagccacagt tccgattatt ttcgggaact
     1501 ggtggatacg catgaacgac tggtgagaga cgctgatgaa cttgtcgcgg tggtaattgc
     1561 tggtttcaat cagatgaatc gtggtggtcc ggcgggaaat gccgtggtgg cgcattgata
     1621 acgtgttctg ggggagggga tgaagctcct ttttgctgaa cgcccgctgg ttataaacac
     1681 gcagctggcg atgaaaattg gtctgaacga agccatcgtg ttgcagcagc tgcattactg
     1741 gttgagagat accggttccg gcatggaatg tgatggtgtt cgctggattt ataacacaac
     1801 agaacaatgg ctggaacagt tcccgttctg gtcagagtca acgttaaaac gcgcatttgc
     1861 aagtctgaaa acgctggggc ttttgcgttg tgaaaagctc aacaaatcaa agcgtgatat
     1921 gactaatttt tacacgatta attacgagag tgagctttta gatggtggca aagtgagcga
     1981 atccatcagg tcaaaatgca ccgctccatc aggtcaaaat gacacgatgg aagaggtcaa
     2041 aatggcacgc tccattggtt caaaacgacc caatgtcatc gggtcaaaat ggcctgatga
     2101 tcttacagag aatacaacag agattactac agagaataaa aacacttctc gtccggaagc
     2161 ttcgcaaccg gacccgcaga cggctgaaca ggatttttta acccgacacc ctgacgcggt
     2221 tgtgttcagt gcgaaaaaac gccagtgggg aagtcaggaa gatttggcgt gcgcacagtg
     2281 gatctgggga cgaattgtga gtctttacga gcaggctgcc agcgatgatg gcgagatctc
     2341 gcgaccgaaa gaaccaaact ggaccgcatg ggccaatgat gtgcgcacaa tgcggatgct
     2401 ggatggcaga actcacagac aaatttgcga aatgtttggg cgggtacagc gggatccatt
     2461 ctgggtaaaa aacatcatga gcccgtcaaa gctccgcgaa aaatgggacg aactggtcat
     2521 ccgcctgggg cgttcacctg tacagcgttg tgtgaatcat atttctgaac cagacaccga
     2581 aattccgccg gggtttaggg ggtaacgaac tgtgaaaaat attgcgacag gcggtgttct
     2641 tgaacgtatc cgtaagctga ctccgcagca tgtaaccgcg ccgtgccgga cagtggacga
     2701 gtggcgagag tggcagcttg cagaagggcg aaaacgtagc gaggagatca accgcctgaa
     2761 tcgtcagttg cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcatcgtaa
     2821 atgctcgttt gcaaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc
     2881 gaaatccatc gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc
     2941 tggtaccggg aagaaccatt tagcggcagc tatcggaaat cgcctgctga aagacggtca
     3001 ggcagtgatt gtggttaccg tggctgatgt tatgagtgcc ctgcatgcca gctatgacga
     3061 cgggcagtca ggcgaaaaat tattgcggga actgtgcgaa gtggatctgc tggttcttga
     3121 tgaaattggc attcagcgcg agacgaaaaa cgagcaggta gtactgcacc agattattga
     3181 tcgacggaca gcgtcgatgc gtagcgtggg aatgctgaca aacctgaact atgaggccat
     3241 gaaaacattg ctcggtgagc ggattatgga tcgcatgacc atgaacggcg gacgctgggt
     3301 gagttttaac tgggaaagct ggcgcccgaa tgtcgtccag ccaggaatca caaagtaatt
     3361 tttaccagga aaaaaattta atggagactg tttttgacgc actgaaagca atgggaaaag
     3421 ccacatccat agaacttgcg gcgcgacttg atgtcagtcg tgaagaagtg cttaacgaac
     3481 tatgggaact gaaaaaggct ggttttgttg ataaaagcgc gtacacctgg cgtgtggctg
     3541 ataacaacgt tcagcaggaa cagccagcgc aggcagagct gccggaagaa accaccacag
     3601 caacagtagc gaaaatctca gagtgcgatt taaccgcgac gattgaacaa cgcggaccac
     3661 aaacggcgga tgaactggct acgttgttcg gtaccacatc acgcaaagta gcttcaacgc
     3721 tggcaatggc aatcagcaaa gggcgtctga ttcgcgtaaa tcagaacggt aaattccgtt
     3781 actgcatgcc ggacgataat ttaccagcaa agccgaaagc cgtatcggta acggaaaccg
     3841 atggtaaagc ctttcctcag ccagccggtg ttgcgttacc ggtacagaaa gatgcaacac
     3901 aggaagatat taaaacagaa actgtggcgg acattgtgca gtcgctgcca tcgtttactg
     3961 caacgcgaga agatgatttg attttgccat cgctgcatat ggcaaatcgc gaactgcgta
     4021 gggcgaagaa tcatgtccag aagtgggagc gagtctgcgc cgcgctgcgg gag
//
LOCUS       DAZKIV010000248         4043 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZKIV010000248 DAZKIV010000000
VERSION     DAZKIV010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.250"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..1694
                     /gene="dpiB"
                     /locus_tag="ACHOT6_004615"
     CDS             36..1694
                     /gene="dpiB"
                     /locus_tag="ACHOT6_004615"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFP9949693.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESKAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1663..2343
                     /gene="dpiA"
                     /locus_tag="ACHOT6_004616"
                     /gene_synonym="citB"
     CDS             1663..2343
                     /gene="dpiA"
                     /locus_tag="ACHOT6_004616"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFP9949694.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKVPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2384..3769)
                     /gene="dcuC"
                     /locus_tag="ACHOT6_004617"
     CDS             complement(2384..3769)
                     /gene="dcuC"
                     /locus_tag="ACHOT6_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFP9949695.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1101 a          955 c         1041 g          946 t
ORIGIN      
        1 gtcaaagagg ctcgggacaa cccgcaagga aaacaatgtt gcagcttaac gagaataaac
       61 agtttgcatt tttccaaaga ctggcatttc cgctgcgtat ctttttgctg attctggtgt
      121 tctcaatatt tgtcattgca gccctggcgc aatattttac ggccagtttt gaggactatt
      181 taacgcttca tgtacgcgac atggcaatga atcaggcgaa aattattgcc tccaatgaca
      241 gtgtcatctc tgcggtgaaa acgcgtgact acaaacggct ggcgaccatc gctaacaaat
      301 tacaaagaga taccgatttt gattatgtgg tgattggcga ccggcactcg atccgccttt
      361 accatcctaa tccggagaaa attggttatc ctatgcagtt caccaaacag ggcgcgctgg
      421 agaaagggga gagctacttc attaccggga aagggtcaat gggtatggcg atgcgcgcca
      481 aaacgccaat ctttgatgac gatggaaaag tcatcggcgt ggtgtcgatt ggctacctgg
      541 tgagtaaaat cgatagctgg cgggctgagt ttttattacc gatggcaggc gtgtttgtcg
      601 tgctgttagg gattctgatg ttgctgtcgt ggttcctggc cgcgcatatc cgtcggcaga
      661 tgatgggcat ggagccaaag caaatcgcac gggtggtccg tcagcaagag gcgctgttta
      721 gttcggttta tgaagggctg attgcggtgg atccgcacgg ttacattacc gccatcaatc
      781 gtaacgcaag aaagatgctg gggctgagtt cccctggacg gcaatggttg ggtaaaccca
      841 ttgctgaagt ggtcaggccc gccgatttct ttaccgaaca gattgatgaa aaacgtcagg
      901 atgtggtggc gaactttaac ggtctgagcg ttattgccaa ccgggaagct attcgttcag
      961 gtgatgattt gctgggggcc attatcagct ttcgtagtaa agacgaaatt tccaccctca
     1021 atgcgcaact gacgcaaata aaacaatacg ttgagagcct tcgtacattg cgacacgagc
     1081 atctcaattg gatgtcgacg ctcaatggtc tgttgcagat gaaagagtat gatcgcgtgc
     1141 tggcgatggt gcagggggag tctaaggccc agcaacagct tattgacagc ctgcgtgagg
     1201 cgtttgccga tcgccaggtg gcggggctgc tttttggtaa agtgcagcgc gcccgcgaac
     1261 tggggctaaa aatgatcatc gtccccggta gccagctttc gcaactgccg ccaggactgg
     1321 atagcaccga gtttgccgcc attgttggca atttacttga taacgccttc gaagccagcc
     1381 tgcgtagcga tgaaggaaac aagatcgttg aattattcct cagcgatgaa ggcgatgatg
     1441 tggtgattga agtcgccgat cagggctgcg gcgttccaga gtctctacga gacaaaatat
     1501 ttgagcaggg ggtcagtacg cgtgctgacg agcccggtga acatggcatt gggttgtact
     1561 tgattgccag ctacgtaacg cgctgcggtg gtgttatcac tctcgaagat aatgatccct
     1621 gcggtacctt attttcaatc tatattccga aagtgaaacc taatgacagc tccattaacc
     1681 ctattgatcg ttgaggacga aacgccgctg gcagagatgc atgcggaata tattcgtcac
     1741 attcccggat tcagtcagat attactggcg ggaaacctgg cgcaggcccg aatgatgatc
     1801 gagcgtttta agccggggct aatcttgctc gataactatc ttcctgacgg tagagggatt
     1861 aatttattgc atgaactggt gcaggcgcat tatcccggcg acgtggtgtt taccactgca
     1921 gccagcgata tggaaacggt gtctgaagcc gtacgttgtg gtgtatttga ttatctcatt
     1981 aagcccattg cctatgaacg gctggggcaa acgctaaccc gtttccgcca gcgtaaacat
     2041 atgctggaaa gtattgatag cgccagccag aagcaaattg atgagatgtt taatgcctat
     2101 gctcgcggtg aacccaaaga cgaactgccg accggcattg accctttgac gctaaacgcg
     2161 gtgcgaaaac tgttcaaagt gcctggtgtg caacatacgg cagaaacggt ggcgcaggca
     2221 ctgaccatca gccgcaccac tgccaggcgt tatcttgaat attgcgccag ccgccatctg
     2281 attattgctg aaattgttca cggcaaagtt ggcagaccgc aacgcatata ccacagtggg
     2341 tgacatgata aagccggagg aaacttgcct ccggcattac tatttacttg cctgtgaccg
     2401 ctgctgccgt tcctggcacc atcagctctg tagcaacgat aacaatcacc agcccaacaa
     2461 gcaccggcac cgaggtgcgt tttacgactt caaatggtga gattttcgcc atcccggcaa
     2521 ccgcaacgac tacgccagaa acgggcgaaa gggtacggcc caggtttgac gcctgcagca
     2581 tcgggatagt caaatacgcc gggttaatgc ctgaagagtg cgccagtttc gggatcatct
     2641 caacaaacgc ataaaacggc gcattgcctg aaccggtcgt gactgccgcc agcattgtca
     2701 gaatcaccaa taccagcatc aggatgatac tcgccgaacc aaacgaggta gcgatagaaa
     2761 tcagactttg aataaagccg atggtgctaa gcccctgagc gaatacccca gcggcaacca
     2821 gcagcatcac cacgtttgca aatgcatctg ccatcccgcg ataagccact tccagaccag
     2881 agaaaacttt ctgggtatta aagctgcgga ggaactccag aatggaggca atcagcatac
     2941 aaatcaccag aatagtgatg atatgtaatt gcggacccca tttgccgtca aaaatcagca
     3001 ccccgatgat cggcgtgaac ggcaaaatgg cataaaacgc aggggcagtg gtggttattt
     3061 cactgacatc taacatttca tgagatatgt gctctttttt atccagataa cgttgccaga
     3121 agaagtgggc gatcgccatg ccgataattg cagcaattga gataggcagt gtcgttttga
     3181 aggcgaagtc aatcagcgac atttcggaag cctgcgccgc cagcaccaca tcccctgaag
     3241 tcggtgcgag aataatcgcc gccggggagg cacaaatggc agcagctgcg ccacgactga
     3301 taccaacgtt taccatcacc ggaaataggg ttgccatcag caaaacaccc agaccggttg
     3361 cggaagagac ggccagagac atcagacagg cgacaaaata ggcggcaatc atcagcaggt
     3421 aaggggagtt aatatactgc aatggttttg acgccagctt gaccaccata tcattcgcgc
     3481 cgatatgggt catgtaagcg gcaaatccac acagcatcat aatcatcatg ccgaggtcgc
     3541 cgccgcggct cattagtaat attttaacgt attcaacgat atccgtggcg ctgtagcctg
     3601 ttgaagcctg gctggacggt aacactttgt gccccataat ggcactgata atcagcaata
     3661 acaggccacc gacaaataac acaccagtgg cggaataccc tttaatgatg tagcgagcta
     3721 cacccacaat aaccacaacc ccaataagga gttcaatgaa tgtcagcata atttttcctg
     3781 tttccaggcc ccaaagtaaa taataaaaaa ttcctaaagc ttaaggaaaa aatatgccca
     3841 ataaattggc gatgaatgct gattaaaatc aagaaaaact gccattaaca cattgaagtt
     3901 gctgttttta tacatagata acaaaacgca tatttagcgg ttggcaatta tcacataata
     3961 atgtgtgtaa attgatggtg gattgatgca aatttgttaa ttgcaatggt gttagctgcg
     4021 gctgtgcgct caaaaaataa tct
//
LOCUS       DAZKIV010000249         3994 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZKIV010000249 DAZKIV010000000
VERSION     DAZKIV010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.251"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..241
                     /locus_tag="ACHOT6_004618"
                     /pseudo
     CDS             1..241
                     /locus_tag="ACHOT6_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(322..480)
                     /locus_tag="ACHOT6_004619"
     CDS             complement(322..480)
                     /locus_tag="ACHOT6_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9949696.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(566..1309)
                     /locus_tag="ACHOT6_004620"
     CDS             complement(566..1309)
                     /locus_tag="ACHOT6_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949697.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1562..2185)
                     /locus_tag="ACHOT6_004621"
     CDS             complement(1562..2185)
                     /locus_tag="ACHOT6_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9949698.1"
                     /translation="MRLESVAKFHSPKSPMMSDSPRATASDSLSGTDVMAAMGMAQSQ
                     AGFGMAAFCGKHELSQNDKQKAINYLMQFAHKVSGKYRGVAKLEGNTKAKVLQVLATF
                     AYADYCRSAATPGARCRDCHGTGRAVDISKTEQLGRVAEKECGRCKGVGYSRMPASAA
                     YRAVTMLIPNLTQPTWSRTVKPLYDALVVQCHKEESIADNILNAITR"
     gene            complement(2182..2847)
                     /locus_tag="ACHOT6_004622"
     CDS             complement(2182..2847)
                     /locus_tag="ACHOT6_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFP9949699.1"
                     /translation="MNIYERIDGSKYRNIWVVGDLHGCYTNLMKKLETIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKGKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(2844..3464)
                     /locus_tag="ACHOT6_004623"
     CDS             complement(2844..3464)
                     /locus_tag="ACHOT6_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFP9949700.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAEKAAEKKRRREEQKQKDKLKIQKLALKPRSYWIKQAQQAVNAFIRERDRDL
                     PCISCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELIS
                     RIGQEAVEEIESNHNRYRWTVEECRAIKAEYQQKLKKLRNSRSEVA"
     gene            complement(3457..3627)
                     /locus_tag="ACHOT6_004624"
     CDS             complement(3457..3627)
                     /locus_tag="ACHOT6_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFP9949701.1"
                     /translation="MIDQNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            complement(3624..3806)
                     /locus_tag="ACHOT6_004625"
     CDS             complement(3624..3806)
                     /locus_tag="ACHOT6_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFP9949702.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHCARKTR"
     gene            complement(3803..>3994)
                     /locus_tag="ACHOT6_004626"
     CDS             complement(3803..>3994)
                     /locus_tag="ACHOT6_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFP9949703.1"
                     /translation="DEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMF
                     TTVSKAALMAIGQGVRRAA"
BASE COUNT         1073 a          998 c          791 g         1132 t
ORIGIN      
        1 aacatcgtaa tgtttaatcg acatcgaatt tctcccgtgt acaggaacag agttaaaaag
       61 ccggaaccgg aatcaaatca caggatgacc atctgccagt ggcaggtcat aaaaaaaagc
      121 tgcgcaatgc gcagccagaa ctcacaagga aaatgataaa aggaataaca ctagtgatgt
      181 acgcatggcg cctcccgcta agttctgcaa tgatcaaaca gaactcgcta cgtgccctta
      241 aaactcgatc atttagcccc tccaaggagg attcaccatg cggttggttt tttaataaac
      301 agtaaacaaa aaagtcaaga attattcatt ctgttctttc atcatcggcc acagcaatac
      361 cacaatgccg cagaccagag cgccatcagt cagtaccaac attatcctgc tggtgaaatc
      421 catcatcacc atcactaaaa gcaggatcac aacagcaagc agacacagtt tataaaacaa
      481 tgttcagaaa acgcattcag catgcctaag gttctattcc tacgaatagc caacttgcaa
      541 cttaaaatat tatttatgca gccaattaaa ttctggtcct tacaatatca acctgaagat
      601 tcttatcttg tgctgattga taaatgacaa accttttact acctgcattg aaagaagtag
      661 acaaaaccag acaattatca taacgagcaa gaacataata ccaaccatca ttataattaa
      721 tcatttcata ttctttctta aactgtggtt tgtaatatcc tgtcagaaat gaaaaaagcc
      781 agaaatatgc cacaaaagca atcatcacaa tctcaaaaaa atgttttttt ataaatggct
      841 tatcatagaa gcatgatacc gataaaaatc gcccataaga tcttatcgaa attgtaaccg
      901 ccagcgcaat cgctgctgac agtagcaaaa gaggtacctg aatcttctgt ctcaatatag
      961 aaaactcaat aattgccggc acaaacaata attccacagc aaaataaagg cgaaatacat
     1021 ttagctcttg catagaatgt tttcttttca ctgcgaaaaa gaatacaaca ccaatacccc
     1081 aaccgataag aaatatagca atgacgataa ctgcaaaaaa taaacttctg gcaacatcat
     1141 caacacctgc acctacaatc caccatggga agccgtagta aaaagaagta ccccatccat
     1201 agaaataagc actcccccat ccaaggcatc ccatgtaggc aataaaaagt gaagaactcc
     1261 tgagcagcgc accatccttc ataaccaccc caatacaaga tgataacatt ggcttacaac
     1321 tcataacaaa agcaattcaa tgccgtcaag aggttacagg ctaaaaaaac tctattacat
     1381 tgcagtcagc atgtttacta cacaaataca attcagagca taaaaactac tcggcggcag
     1441 gttattgaga ctcatcaatg acatgtaaaa aacgcccatt attggtgtca agtttcccca
     1501 aagttattca aaaagtcaat attatgccgt taatatgttg ccatccgtgg caatcatggc
     1561 gctaacgtgt gatcgcattc aaaatgttgt ctgcgattga ctcttccttg tggcattgca
     1621 caaccagagc gtcatacagc ggcttaacag tgcgtgacca ggtgggttgg gtaaggtttg
     1681 ggattagcat cgtcacagcg cgatatgcgg cgcttgctgg cattcttgaa taaccgacgc
     1741 ctttacatct tccgcactct ttctcagcaa ctctccccaa ttgctctgtt tttgatatat
     1801 caaccgcacg gcctgtaccg tggcaatctc tgcatcttgc gcccggcgtc gcggcactac
     1861 ggcaataatc cgcataagcg aatgttgcga gcacttgcag tacctttgcc ttagtatttc
     1921 cttcaagctt tgccacacca cggtatttcc ccgatacctt gtgtgcaaat tgcatcagat
     1981 agttgatagc cttttgtttg tcgttctggc ttagttcatg cttaccgcag aatgcagcca
     2041 ttccgaatcc ggcttgtgat tgcgccatcc ccatagcagc catcacatca gtaccggaaa
     2101 gagagtcaga agccgtggcc cgtggcgagt cgctcatcat cgggcttttt ggcgaatgaa
     2161 atttagctac gctttcgagt ctcatgcgcc ttctccctgt acctgaatca atgtgaggtt
     2221 tccgcagaac actgcgccgg tatctatata catctggttg gcaaacttga gtggtttcac
     2281 tgctggcgta tgaccaaaga tgaacgtgtc cgcgcctttg atttctttca cgatcccgtc
     2341 ttgtgagttg ctgattcgtt cgcggttcca gattacctgc tgatgatcaa ctggctttcc
     2401 aaactcgtat ttatcacaag gataatcggc gtggcagatg acatattttt ttcctttact
     2461 caccagttcg atgattaacg gaagttcatc tgctttatgg gcaagagctt tagccagaat
     2521 ttctttgtcg taatcgagat taaagaacca gccaccgcca ttaagcagcc agtgattgac
     2581 gtttccacgc tctgataagc catcaatcat catttgctca tggtttccac gtacagctct
     2641 gaaccagggg aatgtgatta attccaggca ttctacgttc tctgtaccgc gatcaaccaa
     2701 atcgcccacc gagataagca ggtctttttt ggtgtcgaat cctatcgtct ccagtttttt
     2761 catcaggttc gtgtagcatc cgtgcagatc gccaactacc caaatatttc ggtatttgct
     2821 gccatcaatt ctttcgtaga tattcatgca acctcacttc tgctgtttcg cagtttttta
     2881 agtttctgtt gatactccgc cttgatggcc ctgcactctt cgacagtcca gcgatagcgg
     2941 ttatggtttg attcgatttc ctctactgct tcctgcccga tgcggctaat cagttcgacg
     3001 cgatacggaa cgagatttcc gcttttgtgc tggttgcaca ccacgcattg cttgtgaata
     3061 ttgcgttcat caaatcggag ttgaggtgcc gcagcagttg tccggtaatg tccggcatcc
     3121 cactgagcag acgtgagcgt tccgcacgag atacatggta agtcgcggtc tctttctctg
     3181 atgaaggcgt ttacggcttg ttgggcttgt ttaatccagt aactgcgggg ctttaaggcg
     3241 agtttttgaa tcttaagttt atctttctgt ttctgctcct ctcgtcgtcg tttcttctct
     3301 gctgcctttt ctgccttttc cgctttttcg cgttctttgc ttcgtcgttc gagtgctatc
     3361 ttggttccac actctggaga gcaccaccac tgattagcga atgcagggtg aaaccattcc
     3421 cgacattcat cgtttttaca tcgtcttcgc gctggtttag ccatcatctt cttcctcgtg
     3481 catcgagcta ttcgaatcgc tcatcagctc tgcgcagcag tgctcacaca cgtgaacttc
     3541 cagcacatgc agcttctgac cgcagttagc gcacgttaaa gctcgctcga cgctttcttg
     3601 ttcgtaactt cgattttggt caatcacctt gttttcctcg cacaatgtct tagccaccgg
     3661 atatcccaca ggtgagccgt gtagttgaag gtttttacgt cagattcttt tgggattggc
     3721 ttgcgtttat ttctggagcg tttcgttgga aggtatttgc agttttcgca gatgatgtcg
     3781 gtgaaacttc gtcgctgtcg cctcatgccg ccctcctgac gccctgcccg atcgccatca
     3841 atgccgcttt ggatacggta gtaaacatcc gtcgaggact gatgaacggt cgccaaatca
     3901 gcagcatgga gcctttgctg tttcccttct tctccaaccc tgtcgatggt tcgataaaat
     3961 taatccgtcc atcagtgata atgcgaactt cgtc
//
LOCUS       DAZKIV010000250         3989 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZKIV010000250 DAZKIV010000000
VERSION     DAZKIV010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.252"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..741
                     /locus_tag="ACHOT6_004627"
     CDS             <1..741
                     /locus_tag="ACHOT6_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9949704.1"
                     /translation="QPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRL
                     PRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAG
                     QVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCF
                     KLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLL
                     EDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            897..2432
                     /gene="gadC"
                     /locus_tag="ACHOT6_004628"
     CDS             897..2432
                     /gene="gadC"
                     /locus_tag="ACHOT6_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFP9949705.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAVVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2563..3882
                     /locus_tag="ACHOT6_004629"
     CDS             2563..3882
                     /locus_tag="ACHOT6_004629"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFP9949706.1"
                     /translation="MDICSRNEKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
BASE COUNT          940 a         1022 c          999 g         1028 t
ORIGIN      
        1 caaccgctgc acgatgcgct ggataaattc caggccgaca ccggtatcga catcgacatg
       61 cacatcgacg ccgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
      121 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      181 ccgctgggct gcggctgggt tatctggcgt gatgaagaag cgctgccgca ggaactggtg
      241 ttcaacgttg actacctcgg tggtcagatt gggactttcg ccatcaactt ctcccgcccg
      301 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctatacc
      361 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaactg
      421 gggccgtatg agttcatctg taccggtcgc ccggacgaag gcatcccggc ggtttgcttc
      481 aaactgaaag atggtgaaga tccgggatac accctgtacg acctctctga acgtctgcgt
      541 ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga catcgtggtg
      601 atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt gctggaagac
      661 tacaaagcct ccctgaaata tctcagcgac cacccgaaac tgcagggtat tgcccagcag
      721 aacagcttta aacatacctg ataacgttta acggtaacgg tgtcccgaaa cgaacccgtt
      781 tcgggacaat ttccaaagtc tgttcactgg cattagcaac ggaaaatatt gttctgaata
      841 cgcttcagaa caaaacaggt gcggttccga caggaatacc gttttagggg gataatatgg
      901 ctacatcagt acagacaggt aaagctaagc agctcacatt actcggattc tttgccataa
      961 cggcatcgat ggtaatggct gtttatgaat accctacctt cgcaacatcg ggcttttcat
     1021 tagtcttctt cctgctatta ggcgggattt tatggtttat tcccgtggga ctttgtgctg
     1081 cggaaatggc caccgtcgac ggctgggaag aaggtggtgt cttcgcctgg gtatcaaata
     1141 ctctggggcc gagatgggga tttgcagcga tctcatttgg ctatctgcaa atcgccattg
     1201 gttttattcc gatgctctat ttcgtgttag gggcactctc ctacatcctg aaatggccag
     1261 cgctgaatga agaccctatt accaaaacta ttgcagcact catcattctt tgggcgctgg
     1321 cattaacgca gtttggtggc acgaaataca cggcgcgaat tgctaaagtt ggcttcttcg
     1381 ccggtatcct gttacctgca tttattttga tcgcattagc ggctatttat ctgcactccg
     1441 gtgcccccgt tgctatcgaa atggattcga agaccttctt ccctgacttc tctaaagtgg
     1501 gcaccctggt tgtatttgtt gccttcattt tgagttatat gggcgttgaa gcttccgcaa
     1561 cccacgtcaa tgaaatgagc aatccagggc gcgactatcc actggctatg ttactgctga
     1621 tggtggcggc aatctgctta agctctgttg gcggtttgtc tattgcgatg gtcattccgg
     1681 gtaatgaaat caacctctcc gcaggtgtaa tgcaaacctt taccgttctg atgtcccatg
     1741 tggcaccgga aattgagtgg acggttcgcg tgatctccgc actgctgtta ctgggtgttc
     1801 tggcggaaat cgcctcctgg attgttggtc cttctcgcgg gatgtatgtc acagcgcaga
     1861 aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa tggcgtaccg gtaacgctgg
     1921 tcatttcgca gctggtgatt acttctatcg cgttgatcat cctcaccaat accggtggcg
     1981 gtaacaacat gtccttcctg atcgcactgg cgctgacggt ggtgatttat ctgtgtgctt
     2041 atttcatgct gtttattggc tacattgtgt tggttcttaa acatcctgac ttaaaacgca
     2101 catttaatat ccctggtggt aaaggggtga aactggtcgt ggctgttgtc ggtctgctga
     2161 cttcaattat ggcgtttatt gtttccttcc tgccgccgga taacatccag ggtgactcta
     2221 ccgatatgta tgttgaatta ctggtcgtta gtttcctggt ggtacttgcc ctgcccttta
     2281 ttctctatgc tgttcatgat cgtaaaggca aagcgaatac cggcgtcact ctggagccaa
     2341 tcaacagtca gaacgcacca aaaggtcact tcttcctgca cccgcgtgca cgttcaccac
     2401 actatattgt gatgaatgac aagaaacact aaccatgtct gaatggttat aagataaggg
     2461 agcgataatt catcgctccc tttttcgtgc ttgctgtcgt attgacttaa ccgggataag
     2521 tacgagaatg agcgcacatc tgtttaccgg aaaccagcac atatggatat ctgctcccga
     2581 aacgagaaat taacgattag aagaccagcg atactggttg cactggcact tttactgtgt
     2641 agttgtaaaa gcacgcctcc agagtccatg gtgacaccac cagcaggttc aaagccacca
     2701 gccacgacgc aacaatcgtc acaaccgatg cgtggcatct ggctggccac ggtttctcgc
     2761 ctcgactggc caccggtttc ctcggttaac attagtaacc ccaccagccg ggcccgtgta
     2821 caacaacagg cgatgatcga caaactggat catctgcaac gtctcggtat aaacacggtc
     2881 tttttccagg tcaagccgga cggtaccgcc ctgtggccat cgaaaatttt gccgtggtcc
     2941 gatcttatga ccggtaagat tggtgaaaat ccgggttacg atccgctgca atttatgctc
     3001 gacgaagccc acaagcgtgg gatgaaagta cacgcctggt ttaaccccta tcgcgtatcg
     3061 gttaatacga agcccggtac tatcagggaa ctgaatagca ctctgtctca acaaccggcg
     3121 agcgtctatg tgcaacaccg tgactggatc agaacgtctg gcgatcgctt tgtcctcgac
     3181 ccgggcatcc ctgaggttca ggactggatc acatcaatag tcgcagaagt ggtttcccgc
     3241 tatccggtag atggcgtgca gtttgacgac tatttctata cggagtcacc gggttcacgg
     3301 ctaaatgata acgaaacgta ccgcaaatac ggaggcgcat ttgcgtcaaa agcagactgg
     3361 cggcgcaaca atactcagca gttaattgca aaagtatcgc acaccattaa aagcattaag
     3421 ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc gtaaccgatc acacgatccg
     3481 ctcggttccg atacccgagg tgcggcagcc tatgacgaat cctacgctga cacccgtcga
     3541 tgggtggaac aaggattgct ggattatatt gctccccaaa tttactggcc gttctcacgg
     3601 agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg atgtcgttaa accgaccagg
     3661 acccgcctgt atatcggtat cgccttctat aaagtgggtg aaccttcaaa gatagagcca
     3721 gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc agctcgatct taacgatgcg
     3781 gtacccgaaa ttagcggcac aatcttgttc cgtgaggact atctgaataa accgcagact
     3841 caacaagcgg tcagctatct gcaaagtcgt tggggcagtt aaactcccat tcttactttg
     3901 taacaagccg gtacattacc caccggcttg ttaccctctt gaactatccc actactaatc
     3961 atgcttacta aagtcaaatt aaccatgca
//
LOCUS       DAZKIV010000251         3975 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZKIV010000251 DAZKIV010000000
VERSION     DAZKIV010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.253"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(235..1506)
                     /gene="efeB"
                     /locus_tag="ACHOT6_004630"
     CDS             complement(235..1506)
                     /gene="efeB"
                     /locus_tag="ACHOT6_004630"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFP9949707.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(1512..2639)
                     /gene="efeO"
                     /locus_tag="ACHOT6_004631"
     CDS             complement(1512..2639)
                     /gene="efeO"
                     /locus_tag="ACHOT6_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFP9949708.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(2697..3527)
                     /gene="efeU"
                     /locus_tag="ACHOT6_004632"
     CDS             complement(2697..3527)
                     /gene="efeU"
                     /locus_tag="ACHOT6_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFP9949709.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(3785..>3975)
                     /locus_tag="ACHOT6_004633"
     CDS             complement(3785..>3975)
                     /locus_tag="ACHOT6_004633"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949710.1"
                     /translation="RRALSPHPYGLPRPSGNASAILTPPTPALFLCSGQKVRFDNRGW
                     TKLLDTPAYQFYMEVLMY"
BASE COUNT          982 a         1108 c          947 g          938 t
ORIGIN      
        1 ataaaacacc atttcagtga cattagtttc tactggaaag atgacagagt gatgacagat
       61 atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca cgatcgccgt
      121 atgcggcgtg aacgccttat ccgcctaccg aacccgcacc gattgtaggc ataagaagac
      181 gcgcaagcat cgcatcaggc atctgcgcaa gaacgcctta tccgtctaaa aacattaaac
      241 ccgcaataac gcgcttccga gataatcgtt cgcgtccttc actcccggca gcgcaaaaaa
      301 ataaccgccg ccgataggtt taacgtattc ctccagcgct tcgccattga gccttttttg
      361 tactgtcagg aagccttttt ccagatcgtg ttggtaacag acaaacagta gccccatatc
      421 cagttgcccg gagttggtga cgcccagtga ataactgtag ccacgacgca gcatcaggct
      481 ggactcactc tccgccgtgc ggggattcgc cagccggata tggctgtcca gcgcaatcac
      541 cttcccttcc gggtcgctgg cgtaatcagg cacatcatgc tcatgctgca ttcccagcgg
      601 cgcaccggtt tgcttatcac ggccaaaaat cgtctgctgt tctttcagcg gcgttctgtc
      661 ccaaaattcc actcgaaact gaatcaagcg tactgcctga tagctgccac cgattgtcca
      721 cgcaggctcc tgctgatctg ccgttaccca caccactttt tgcatcaact tatcattctg
      781 gctatcggga ttggcagtgc cgtctttgaa acccagcaaa ttaatcggcg tctctttgcc
      841 tttactacgc gccgcgtgat cggaaataaa cccttcccgc ttccagcgca cactgagcaa
      901 atccggcgtg tgtttgatga tatcgcgcag cgcatggata accgtgtcct gggtgttggc
      961 gcaaatctgt agcaacacat caccatgaca taacgccgca tccagcgagt cgttggggaa
     1021 acgcgtcatc ttctgcagct tttttggcat ctgtggcgca aggccaaagc gctcatcaaa
     1081 caatgagtga cccaccgata acgtgatggt gagattatcg ggcgcaatgt agccgccaag
     1141 aatgccggaa tcgagtggtg gcaggcgcgg atttggcgtt tctggtgctg ctccgccctg
     1201 agtcagaaaa gcaaaacgct gagtcaacaa gcgaaacaac cgctcaagat cggctttatc
     1261 gctggcaagc acatcaaacg ccaccagcat cattgcggcc tgttgtggcg tcaggatccc
     1321 tgcctgatgc tcaccataaa acggctgttt ctcattgcga gcatccggtg aaagcgtgcc
     1381 cggtgcactt tgcgtttttt gtgcatgagc gaccggacaa cttcccgcca gcgccagcgc
     1441 accaatccct ttcagtaaac gtcggcgtga cggttcattc acgccgtttt catctttata
     1501 ctgcataacg cttaatccag tcccagcaca ccgcgaagtt gcgccagatc ttccgccagc
     1561 gcagtaatcg gccctttcag cgcattccgg tcggcatcgg tcaatttgtc gtaggtttca
     1621 aaaccgtctt tagtacggta tttcgccaga atggtatcga cctttttaaa gttggcatcg
     1681 acttttgcca acagttccgg gttggctttt tgcagttgtg gacgcagcag atcgacaatt
     1741 ttctgcgagc cttcaacgtt agcctggaaa tcccacagat cggtgtggct gtagcgatct
     1801 tcttcaccgc tgattttgct ggctgccact tcctcaatca gtccggctgc gccgccgacc
     1861 acttttgaag gtgggaaagc cagttcactg atgcgttttt gcaaatcgac cacatcggta
     1921 taaagctgct cagcgtactg atccatccct ttggtggtgt tgtcgccaaa caatgctttt
     1981 tccagacggt ggaaaccagt gaattttggg tcggcggctt tttgctcgta atcatcttca
     2041 cgggcgtcaa tgctgccatc cagatcggag aacagttcag caatcggttc aatgcgctca
     2101 tagtgctggc gcgtcggcgc atacagtgct ttcgcttttt cgatatcgcc agctttaatc
     2161 gcgtcggtaa aggctttggt gtcggtcacc agctgcgtgg tttccgccat gacatacgct
     2221 ttatatgcag taattgcacc accaagactt aacagcgcat cactttgcgc cgcatccgcc
     2281 gttgcctcac ctttgacgat caacttccct ttcgggttag tcagcagacc gcaggtcata
     2341 tcgtattcgc caggctgtaa attcgccgtc attttctggc taaaaccagg ggcgatattt
     2401 tcccgctctt ccaccaccat cacgcctttg aggatctccc actccagcgc cttctggcta
     2461 tggttctgaa taatgaactg tgttttcccg gcgttaaccg taatggtcat cggttcgcac
     2521 tgcttatccg tcacggtcac tttgacctgc gtcacatcag cggcgttagc cataaaagca
     2581 gaagaaaaca gcgcagccac gctcaactgc aatgcgttac ggcggaagtt aatggtcatg
     2641 acgagactat ccctttaaag tatgttgtaa ctaagcaaga gtttgcgtcg tatttgttac
     2701 gcggagcgag acgctgtcgc ccctgcgcgt ggtggcagag caaatgccac cagcgccggg
     2761 atgagataaa taaaccagac ggcgacttcg ctgacgctcg gcgcttcctg atagccaaaa
     2821 atcccttcca tcagcgtgcc aaacagcgag tgagttgaga gcaccgcact catatcgaag
     2881 gcgatttcct gaaagtggtt ccacaatccg gcttcatgaa atgcgcgaat ggcaccagct
     2941 gccagccctg cggcgacgaa gagaataaac aggctggtcc atttaaaaaa tgcaccaaga
     3001 ttgaggcgaa taccgcccca gtagagcagg aagcctagca ccacggcagt agcaagaccg
     3061 agcattgcac ccagcggcgg ccagatcccg acatcttgtt gaaatgccgc cagcaggaaa
     3121 aagaccgact ccagcccttc ccttgcaacg gcaaaaaaga ccatcatcac cagcgcccag
     3181 ccatgatgat ttccacgctg caatgcacta tcgactgcct gctccagttg tactttgacg
     3241 ttgcgcgaca ctttgcgcat ccagaaaacc atccaggtaa ggatcaccac ggcgatcacc
     3301 gccacgatac cttcaaacag ttcctgctct ttttgcggaa attcgccggt ggtttcgtta
     3361 atgaagatcc ccaagcccag gcacaacgca gcggcaagca acacgccaat ccacatcaca
     3421 ccaatccatc ggcctcgctg ggtacgctta agatagctgg caatcaaact gacaatcagc
     3481 gcggcttcaa gtccttcgcg caacataatg agaaacggaa caaacatgcc acccaccctt
     3541 aaacgttttt ctgagtcaat ctatgcaaag aaaagtaaat cgcagtgata gcgattatca
     3601 ttacggcaaa gaaaaataca agcggaatgt ttataaaatg atgttgaaat gtaaacaaag
     3661 agaggtaatg ccaggggata taaacgatta ttctaggtac aaagatggga ttgtttacaa
     3721 cgagccatgt gttctcgccg gatgccgcgt aaacgccgta tccagccgac aaaaccgtcc
     3781 tgactcaata catcaaaact tccatgtaaa actgataagc gggtgtatca agcaatttcg
     3841 tccatcctcg attatcaaat ctaacttttt gcccggaaca taagaacagc gcgggtgttg
     3901 gtggcgtcaa aatcgctgaa gcgttccccg aaggccgggg cagcccatat ggatgtgggc
     3961 tgagggcgcg tctta
//
LOCUS       DAZKIV010000252         3967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.254, whole genome shotgun sequence.
ACCESSION   DAZKIV010000252 DAZKIV010000000
VERSION     DAZKIV010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.254"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(60..338)
                     /gene="escG"
                     /locus_tag="ACHOT6_004634"
     CDS             complement(60..338)
                     /gene="escG"
                     /locus_tag="ACHOT6_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle protein
                     cochaperone EscG"
                     /protein_id="HFP9949711.1"
                     /translation="MVNDVSANKILVWAAVAAANHKLPKYAEAILNVFPQIIPDKKDI
                     AHLEFIILFGLNRKNDAVKALENCMDDETSQLLYSLVHENGSGWVRGF"
     gene            complement(344..565)
                     /gene="escF"
                     /locus_tag="ACHOT6_004635"
     CDS             complement(344..565)
                     /gene="escF"
                     /locus_tag="ACHOT6_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE needle filament
                     protein EscF"
                     /protein_id="HFP9949712.1"
                     /translation="MNLSEITQQMGEVGKTLSDSVPELLNSTDLVNDPEKMLELQFAV
                     QQYSAYVNVESGMLKTIKDLVSTISNRSF"
     gene            complement(600..1007)
                     /gene="cesD2"
                     /locus_tag="ACHOT6_004636"
     CDS             complement(600..1007)
                     /gene="cesD2"
                     /locus_tag="ACHOT6_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LcrR family type III secretion system chaperone
                     CesD2"
                     /protein_id="HFP9949713.1"
                     /translation="MVDTFNDEVLNHYLEQKGYTIQKEFLCGSAFFIGWRIETPFFSL
                     AYRLDEQELILCSFEARNQTGLNGPVLSLTRLLEELYHHFSGIKKISAMKSKIGSDSE
                     RQKREELFNYFIRKGAVQQETEDGIWFIMNVNS"
     gene            complement(1014..1976)
                     /gene="espB"
                     /locus_tag="ACHOT6_004637"
     CDS             complement(1014..1976)
                     /gene="espB"
                     /locus_tag="ACHOT6_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     pore-forming subunit EspB"
                     /protein_id="HFP9949714.1"
                     /translation="MNTIDNNNAAIAVNSVLSSTTGSASSTTTSTSSISSSLLTDGRV
                     DISRLLLEIQKLLREMVTTLQDYLQKQLAQSYDIQLAVFESQNKAISEKKAGATAALI
                     GGAISSVLGILGSFAAINSATKGASDAVQQAASTSAKSISTASEASTKALAKASESIA
                     DAADDAASVQQTIAAAAKAASRTSGVTDDVAASAQKASQVAEEAAGAAQELAEKAGLL
                     SRFTAAAGRISGSTSFIVVTSLAEGTKTLPTTISESVKSNHDISEQRAKSVENLQASN
                     LENYKQDVRRTQDDISSRLRDMTTTARDLTDLINRLGQSARLAG"
     gene            complement(1997..3139)
                     /gene="sctE"
                     /locus_tag="ACHOT6_004638"
     CDS             complement(1997..3139)
                     /gene="sctE"
                     /locus_tag="ACHOT6_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     SctE"
                     /protein_id="HFP9949715.1"
                     /translation="MLNVNSDIQSVMSGVSAATTTSGINQPGGSSALDLQLVKSAAPS
                     ASWTESTALPKPPAGHSLVTPSVAEDVLSKLFGGISGDVMSRTSGTEPQSSTQSTTSG
                     YPYLSQVNKVDPQTMMMMATLLSLDASAQKVASMKNSNEIYKDGQNQALENKTLEFKK
                     QLEEQQKAEEKAQKSKIVGQVFGWLGVAVTAVAAIFNPVLWAVVAVSATAMALQTAVD
                     VMGDKAPQALKTAAQAFGGLSLAAGILTAGIGGVSSLMSKVGDVAGKVGSNIVKAVTT
                     LADTLVENVASKISAVANGLTTSSRSIGTTVLNNDAANHNLLSQISSFVVENLTRQSE
                     SLSQSAKAELDKAALEVQNQANYIQSASKLISDSARVNSRIVSGRV"
     gene            complement(3152..3730)
                     /gene="espA"
                     /locus_tag="ACHOT6_004639"
     CDS             complement(3152..3730)
                     /gene="espA"
                     /locus_tag="ACHOT6_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocon
                     filament protein EspA"
                     /protein_id="HFP9949716.1"
                     /translation="MDTLNTASVASTNASTSTSLTYDLGSMSKDQVVELFNKLGVFQA
                     ALLMFSYMYQAQSDLSIAKFADMNEASKASTTAQKMANLVDAKIAEVQSSSDKNAKAQ
                     LPKEVLDYINDPRNDVTISDITDLNAALGAGNLQTVKAAISAQANNLTTTVNNSQLEI
                     QQMSNTLNLLTSARSDMQSLQFRTVSSISLGK"
     gene            complement(3789..>3967)
                     /locus_tag="ACHOT6_004640"
     CDS             complement(3789..>3967)
                     /locus_tag="ACHOT6_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TyeA family type III secretion system gatekeeper
                     subunit"
                     /protein_id="HFP9949717.1"
                     /translation="LLESPSRVSIVEDKHVYYFHLLKDFFASLPDACFIDNEQRDNTL
                     LMIGKVIDYKEDVM"
BASE COUNT         1136 a          923 c          744 g         1164 t
ORIGIN      
        1 ctggcgaact ctcataatat atcaaaacaa ataattaaat tattttaaat caatataaat
       61 taaaatcctc gtacccagcc actaccattc tcatggacca aactgtacaa taactgactc
      121 gtctcatcat ccatacagtt ctccaaagcc tttaccgcat cattttttct atttaatccg
      181 aataagataa taaattctaa atgtgcgata tcttttttat caggtataat ttgcggaaat
      241 acattcagga tagcttccgc atatttggga agcttatgat ttgccgcggc tacagctgcc
      301 cacaccagta tcttattggc agaaacatca ttaaccataa aaattaaaag ctacggttgg
      361 aaatggttga gaccagatct tttatcgttt tcagcattcc actttctacg ttaacataag
      421 cagaatattg ctgaaccgca aactgcaact ctaacatttt ttcagggtca ttaaccaaat
      481 cggtgctatt aagtaactct ggcacagaat cgctcagtgt tttacctacc tcacccattt
      541 gttgagtaat ttcagataaa ttcattaaat ctccttaata gttaataaaa taaaaatccc
      601 taactattta cattcattat gaaccaaatt ccgtcttctg tttcttgctg cacggcaccc
      661 tttctgatga aataattaaa caactcttcg cgcttttgac gttctgaatc tgaaccaatc
      721 ttagatttca tcgcactgat tttcttaata cccgaaaaat ggtgatacaa ctcttcgagc
      781 aaacgagtca gtgataaaac agggccgtta agccctgttt ggttacgtgc ttcaaaagag
      841 cacaaaatca gttcttgttc atccagtctg tacgctaatg agaaaaaagg tgtctcaatc
      901 cgccatccga taaaaaaggc actgccacaa agaaactcct tctgtattgt gtaccctttt
      961 tgttcaagat agtgattaag cacttcatca ttaaacgtat cgaccatgat caattaccca
     1021 gctaagcgag ccgattgccc caggcgatta ataaggtcgg taaggtcacg ggctgtcgtt
     1081 gtcatatcac gcagacggct agagatatca tcctgcgttc tgcgaacatc ctgtttgtag
     1141 ttctctaaat tcgacgcctg gagattttct acagatttag cacgctgctc actgatatca
     1201 tgattggact tgacagattc agatatcgtt gttggcaatg ttttcgttcc ttcagcaagg
     1261 ctggtaacaa caataaatga cgtagaaccg gaaatccttc cagcagcagc agtaaagcga
     1321 cttaataatc ctgccttctc tgctaattct tgagcagcac cagcagcctc ttcagctact
     1381 tgagaagctt tctgagcaga agcagccaca tcatcagtga caccagatgt acgactggcc
     1441 gcttttgcag ctgccgcgat agtttgctgc actgaggctg catcatctgc tgcatctgca
     1501 atactttcgg aagccttcgc cagtgcttta gttgaagctt cagaggctgt actgatagac
     1561 ttcgcagaag tagaagcggc ttgctgaacg gcatcactcg ctcctttcgt tgcgctatta
     1621 atggcagcaa aagagcctaa aatccccagg acagatgaga tagcaccacc gatcagagcg
     1681 gctgtcgcac cggctttttt ctcactaata gctttatttt ggctctcaaa aacggctagt
     1741 tggatgtcat agctttgcgc caattgcttc tgaagataat cttgcaatgt ggtgaccatc
     1801 tcacgcaaca gtttttgaat ctccagcaga agcctagaaa tatccactct gccatcagta
     1861 agtaaagatg aactgattga cgaggttgat gtcgttgttg aactggcgct gccagtcgtg
     1921 ctgctcaaaa cagaattaac tgcgattgcc gcattgttat tatcgatagt attcattata
     1981 aacgcctctt taacaattaa actcgaccgc tgacaatacg gctattcact cgcgcagaat
     2041 cagaaattag cttagaagca ctctgtatat aattcgcctg gttttgcacc tcgagtgcgg
     2101 ctttatccag ctctgccttt gcactctgac ttaagctttc actttgtcga gttaaatttt
     2161 ccacaacaaa agaagaaatc tgtgataaca gattatgatt cgctgcgtca ttattcagca
     2221 cagttgtacc aatagagcgc gaagaagtgg ttaaaccatt tgccacagcc gaaattttcg
     2281 atgcaacgtt ttcgacaaga gtgtcggcca acgtagtgac cgcttttacg atgtttgaac
     2341 ccactttgcc agccacatcg ccaactttag acataagtga agacaccccg ccaatgccgg
     2401 ctgtcagaat acctgcggca agagatagac cgccaaaagc ttgcgctgct gtttttaacg
     2461 cctgcggagc cttatcgccc ataacatcaa ccgcagtttg cagcgccatt gctgttgcac
     2521 taacagcaac aacagcccag agaactgggt taaaaatagc tgcaacagca gttaccgcaa
     2581 cccctaacca accaaagacc tgaccaacaa ttttactttt ttgtgctttc tcttcggctt
     2641 tctgttgttc ttcaagctgt tttttaaatt ccagagtttt attttccagc gcttgatttt
     2701 gtccatcctt gtagatctcg ttggagttct tcatactcgc aactttctgc gctgaggcat
     2761 ccagggataa cagagtggcc atcatcatca ttgtctgagg gtcaacctta ttcacctgag
     2821 aaagataagg atagccagag gttgtacttt gtgtactact ctgtggttct gtcccactag
     2881 tacggctcat aacatcacca ctaatgccgc caaaaagttt gctcaaaaca tcttcagcaa
     2941 cagagggcgt cactaatgag tggcctgccg gcggctttgg taaagcggta ctttctgtcc
     3001 aacttgctga tggggcagcg gatttaacca gctgtaaatc cagcgcggaa gaccctccag
     3061 gttgattaat acctgaagta gtcgtagcgg cactgacccc agacatcaca gactggatat
     3121 cgctatttac attaagcata gttatctccg gttatttacc aagggatatt gatgatacag
     3181 ttctgaattg cagtgactgc atatcagaac gtgcactcgt taataggttt aacgtatttg
     3241 acatttgctg tatttcaagc tggctattat tcaccgtcgt tgtcaaatta ttcgcttgag
     3301 ctgaaatagc tgctttcacc gtttgcaaat taccagcgcc taatgcagca tttagatcgg
     3361 taatatcact tattgtaacg tcattgcgag gatcatttat atagtcaagc acttctttag
     3421 gaagttgagc cttcgcattc ttgtcggagc tactctgaac ctcagcaatt ttagcatcca
     3481 caagattcgc cattttttgg gcagtggttg acgccttaga tgcctcattc atatcagcaa
     3541 actttgcaat cgacagatcg ctttgtgcct gatacatata ggaaaacatg agaagcgcag
     3601 cctgaaaaac accgagttta ttgaatagtt caacaacctg atctttcgac atgctcccta
     3661 aatcataggt cagcgatgtc gaagtactcg cattcgtact agcaactgat gctgtattta
     3721 atgtatccat gtatatacct cttaataatc tttcttagca tgacaaaaac tattaaaaaa
     3781 caattaaatc acataacatc ctccttataa tcaattactt tgccaatcat taataatgta
     3841 ttatccctct gctcgttatc aataaaacaa gcatcgggta atgatgcaaa aaagtctttc
     3901 aataaatgga aataataaac atgtttatct tcgactatcg atacccgaga agggctttcc
     3961 agtagcc
//
LOCUS       DAZKIV010000253         3923 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZKIV010000253 DAZKIV010000000
VERSION     DAZKIV010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.255"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1128
                     /locus_tag="ACHOT6_004641"
     CDS             <1..1128
                     /locus_tag="ACHOT6_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFP9949718.1"
                     /translation="TTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFC
                     DEHGRVRVKFNWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDP
                     DQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQ
                     KNMNTEVLNNRTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSN
                     QIIKVGSNQVEKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYS
                     VNVGNNVTFSVGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLE
                     GESDVNGDAPVINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            1204..>3923
                     /locus_tag="ACHOT6_004642"
     CDS             1204..>3923
                     /locus_tag="ACHOT6_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFP9949719.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSP
                     IEEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQVHERELILNDSGGRSIHFEPLFPGEVSYSRSESFWLARGGVAEQHSSQPLS
                     ALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPPPPAYRVLTGVVD
                     GFGRTLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRATSLSSPAS
                     PRSVSSSQVFPDTLPAGTEYGVDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGE
                     LRAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYR
                     FEYGQDRVTITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDA
                     AGRRTEYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDEKGRL
                     AAETSRSGETTSYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYE
                     YDRYGQQIAVHREEGISTYSSYNPRGQMVSQKDAQGRETRYEYSAAGDLTATVSPDGK
                     RSTIAYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRG
                     FDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITHRTVNGDPAEQWQYDEHGWLT
                     TLSHTSEGHRVSVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVT
                     PDSLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTST
                     YTPAGQLQSQHLNLPQLDREYTWNDNGQLVRISGPQESREYRYSDTGRLE"
BASE COUNT          903 a         1037 c         1335 g          648 t
ORIGIN      
        1 accacgctga ataatcactt tgcggtgatc ccggcagaca gaacatggcg accacagccg
       61 ttgctgaaac cgctggtgga cggcccgcag agcgctgtcg tgacgggacc ggcaggcgag
      121 gaaatcttct gtgatgaaca tggccgcgtg cgggtgaaat ttaactggga ccgttataac
      181 ccgtcaaacc aggacagttc atgctggatc cgtgtggcac aggcgtgggc aggcaccgga
      241 ttcggtaacc tggcgatacc gcgtgtgggt caggaggtga ttgtggactt cctcaacggc
      301 gatccggacc agccgatcat tatggggcgc acctaccacc aggaaaaccg cacacccggc
      361 agcctgccgg gaacaaagac gcagatgacc attcgttcga aaacctataa gggcagcggg
      421 tttaatgaac tgaagtttga cgatgcgaca gggaaagaac aggtctacat ccacgcgcag
      481 aagaacatga acaccgaggt gctgaataac cgcaccacgg atgtgataaa caaccatgcc
      541 gaaaaaatag gtaacaacca ggcgatcacc gttaccaata accagatcca gaacattggc
      601 gttaatcaga tacagacggt tggtgtcaac caggtggaaa cggtgggcag taaccagatt
      661 atcaaagtgg gatcaaacca ggttgaaaag gtggggatca ttcgtgcgct gacggtgggt
      721 gtggcttacc agacgacggt aggcggcatt atgaatacct cggtggcgtt gttgcagtcc
      781 tcacaggtag ggctgcataa atcactgatg gtgggaatgg gctacagcgt caatgtgggg
      841 aataacgtca ccttctcggt gggcaagacg atgaaggaaa acaccggaca aacagcagtt
      901 tattctgccg gtgagcatct tgaactctgc tgtggtaagg caaggctggt gctgacgaag
      961 gacggaagca tatttctcaa cggtacgcac attcatctgg aaggggagtc ggatgtgaac
     1021 ggtgatgcgc cagtgattaa ctggaactgt ggtgccacac aacctgtacc ggatgcgcct
     1081 gtgccgaaag atttaccccc cggaatgccg gatatgcggc aattttgagt caggtgcaaa
     1141 aaattaaaac ggaactggtg cagtgaccag atgaaccata ttaaaagcag aaggaattga
     1201 ttcatgggcg gcggaaaacc ggcagcgcgg cagggtgaca tgacccgcaa gggactggat
     1261 attgtgcagg gttcagcagg ggtgctgata ggcgcgccga cgggagtggc ctgttcggta
     1321 tgtcccaaca gcccgataga ggagcagaag ggtaacccgg tgaacccgct gctgggagca
     1381 aaggtgctgc cgggcgagac ggaccttgcg ctgcccggcc cgttgccgtt tattctttcc
     1441 cgcgcctaca gcagttaccg gaccaggacg cccgcgccgg tgggggtgtt cggtcccggc
     1501 tggaaagcac ccttcgatat ccgcttacag gttcatgagc gggaactgat actcaacgac
     1561 agcggcggca ggagcattca ctttgagccg ctgtttcccg gcgaggtaag ctacagccgc
     1621 agcgagtcgt tctggctggc gcggggcggg gtggcggagc agcacagttc gcagccgcta
     1681 agcgcgctct ggcaggtgct gccggaagat gttcgcctga gtccgcatgt gtacctggcg
     1741 acaaacagcc ttcaggggcc gtggtggata ctcagctggc cggagcgggt gccgggggcg
     1801 gacgaggtgc tgccgcctcc gccgcccgca taccgggtgc tgacgggggt ggtggacggc
     1861 ttcgggcgga cgctggcctt tcaccgggca gctaagggtg acgtggcggg cgcggtgacg
     1921 ggggtgacgg acggcgcggg ccgccgtttt cacctggcgc tgaccacgca ggcgcagcgg
     1981 gcggaagcct tccgtaaaca gcgcgccacg tctttatctt ccccggccag tccccgttct
     2041 gtttcctctt ctcaggtttt ccccgacacg ctgcccgccg gtacagagta cggtgtcgat
     2101 aacggtatcc ggctggaggc ggtgtggctg acacacgacc cggcataccc ggatgaacag
     2161 ccgactgcgc cgctggcgcg ctacacgtac acggccggtg gtgaactgcg ggcggtgtat
     2221 gaccgcagcg gaacgcaggt gcgcgggttt acttatgatg cggagcacgc cgggcggatg
     2281 gtggcgcacc attatgcagg gagaccggag agccgctacc ggtatgatga taccgggcgg
     2341 gtgacggagc aggtcaaccc ggaggggctg gactatcgct ttgagtacgg gcaggaccgt
     2401 gtgaccatca cggacagcct gaaccggcgg gaggtgctgt acacggaggg cgaaggcggc
     2461 ctgaagcgtg tggtgaagaa ggagcatgcg gacggcagca tcacccgcag cgagtatgat
     2521 gaggcgggga ggctgaaggc gcagacggat gcggcgggac ggcggacgga gtaccgactg
     2581 catatggcgt ctggaaagct gacttcagtg atactgccgg atggcaggac ggtacggtat
     2641 ggctataaca gccagcggca ggtgacgtcg gtgacgtacc cggacgggct tcgcagcagc
     2701 cgggagtatg atgagaaggg aaggctggcg gcggagacct cacgcagcgg tgagacgacg
     2761 agttacagct atgatgaccc ggcgagtgag ctgccgacag ggatacagga cgcgacgggc
     2821 agtacaaaac agatggcatg gagccgttac ggtcaactgc tggcctttac ggactgctcg
     2881 gggtacacga cgcggtatga gtacgaccgg tacggtcaac aaatcgccgt tcaccgggaa
     2941 gaaggcatca gcacttacag cagttataac ccgcgtgggc aaatggtcag tcagaaggat
     3001 gcgcaggggc gtgaaacccg ttatgagtac agcgccgcag gagacctgac agcaacggta
     3061 tcaccggacg gtaaacgcag caccatcgca tatgataaac gcgggcgtcc ggtgagtgtg
     3121 acggaaggcg gtctgacgcg cagcatgggg tatgacgctg ccgggcgcat caccgtgctg
     3181 accaacgaga acggcagcca gtccacgttc cggtatgacc cggttgacag gctgactgaa
     3241 cagcgcggtt ttgacggccg gacgcaacgt taccagtatg acctgaccgg aaaactcacg
     3301 cagagtgaag acgaggggct tatcaccctc tggcactacg atgcgtcgga ccgcatcacg
     3361 caccggacgg tgaacggcga cccggcagag cagtggcagt atgatgagca cgggtggtta
     3421 accaccctca gccataccag tgaaggccac cgcgtgtcgg tccactacgg ctacgacgat
     3481 aagggccgcc tgacgggcga acggcagacg gtggagaacc cggagacggg ggaactgctg
     3541 tggcagcatg agacgaaaca cgcctacaac gaacaggggc tggcaaaccg cgtcacgccg
     3601 gacagcctgc cgccggtgga gtggctgaca tatggcagcg gttatcttgc ggggatgaag
     3661 ctgggcggaa cgccgctggt cgagtacacg cgggacaggc tgcaccgtga gacggtgcgc
     3721 agcttcggca gcagggcagg cagtaatgcc gcatatgaac tgaccagcac atacaccccc
     3781 gcaggccagt tacagagcca gcacctgaac ctcccgcagc ttgaccgtga gtatacgtgg
     3841 aacgacaacg ggcaactggt gcgcatcagc ggcccgcagg agagccggga gtaccgttac
     3901 agcgacacgg gcaggctgga gag
//
LOCUS       DAZKIV010000254         3856 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZKIV010000254 DAZKIV010000000
VERSION     DAZKIV010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.256"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..221)
                     /locus_tag="ACHOT6_004643"
     CDS             complement(<1..221)
                     /locus_tag="ACHOT6_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949720.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGASAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARI"
     gene            complement(233..586)
                     /locus_tag="ACHOT6_004644"
     CDS             complement(233..586)
                     /locus_tag="ACHOT6_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFP9949721.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDDVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            complement(598..993)
                     /locus_tag="ACHOT6_004645"
     CDS             complement(598..993)
                     /locus_tag="ACHOT6_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFP9949722.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTSVSPENALTGHENEVVSAQTDTVTDTAALVTVVALVTLHTDALHATRDEP
                     VAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            complement(1035..2060)
                     /locus_tag="ACHOT6_004646"
     CDS             complement(1035..2060)
                     /locus_tag="ACHOT6_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFP9949723.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGGTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVRDKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            complement(2116..2448)
                     /locus_tag="ACHOT6_004647"
     CDS             complement(2116..2448)
                     /locus_tag="ACHOT6_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFP9949724.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKASAMTPLMLDTAT
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            complement(2458..3777)
                     /locus_tag="ACHOT6_004648"
     CDS             complement(2458..3777)
                     /locus_tag="ACHOT6_004648"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFP9949725.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPGDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVPAM
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            complement(3758..>3856)
                     /locus_tag="ACHOT6_004649"
     CDS             complement(3758..>3856)
                     /locus_tag="ACHOT6_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949726.1"
                     /translation="AAGLKPPAWAAAAFESGLRQSTEEEKSDSRAA"
BASE COUNT          769 a         1188 c         1054 g          845 t
ORIGIN      
        1 ttgattctgg cctgcggatt tttaaccgtg gcccttttca gcctggccct ttcctttacc
       61 agtttccggc gtacctttgt ctcacgggca acctgtgacg cagactgcga tatcgcggat
      121 gaagcaacgc ggttaatggc cattgctgac gcaccgggca ccgccgtttt gctgatacgg
      181 ctgaggtttt caacggcctg ctcaagacct tttatggcca tacatccccc tttcagcggc
      241 gacggttaac ggcaggcggc acgccacgcc caagccagag atgacagctt ccgccatcat
      301 ccggcgaaat ccggtctatc cagaagtttt cctcaccgat ggtcagcgtg tctccacgcc
      361 gcagttgccg cacatcatca gtccggacaa acagggacgg gctggagcct tcaacgcgca
      421 cgccctgtcc ggcatagctg atattttcag ggtcatcaaa aacaccacgt attactgcgc
      481 cggactgctc accggatgtc atggtggctg acgttcccat gtacccgcgt atcgtttcat
      541 cggcgcgggc aatggcagca tcgaacaggt tatcgaaatc agccacagcg cctcccgtta
      601 ttgcattctg gccaggccac gttctgtcat ttcggctgcc acaccggcag agacacgaaa
      661 cgccgttccc ggcagcacaa atgccacagg ttcatcccgc gtggcgtgaa gtgcatcggt
      721 atgcagcgtc accagtgcca caaccgtgac cagagcagcc gtatcagtca cggtatccgt
      781 ctgtgctgat accacctcat tttcatgtcc ggtcagcgca ttttccgggc tgacagacgt
      841 gtcctgaccg gctgcgtcat ccgtgtcatc aagctcctct tccagctctg ccacacggag
      901 cgccagttct tctttcgtcc ccgtcaggct gacatcacgg ttcagttgtt cacccagcga
      961 gcggagacgg gcaatcagtt catctttcgt catggactca tccacagaga aacaatggcc
     1021 ccgaagggcc atgattacgc cagttgtaca gacacgaact catcagggtc agccagcagc
     1081 atcagcggtg ctgactgaat catggtgaac tctcgcgccg gatcgccggt agtcacccag
     1141 tttttcgggt aacgggcaga ggcgttaatg ccttcgcgct gtgcgtccgc atcctgaata
     1201 cagccatagg tacgcagacc gcgtgcctga gtgttcccca gcaccatcgt gttgtccggc
     1261 aggaagttct ttttgacgcc gttttccacg tactgtccgg aatacacgac gatggccaca
     1321 tcgccataca ttcccttata agacaccgct ttgcccaggt cttttaccgc tgtctccagc
     1381 tcggaatgag agccgcgacg ggtatccagc ttatccctga cggctttgaa ggaacggaac
     1441 agcgcccatc ctttcggatc aaacacgatg atattcacca cgccgctggc gttcagcgcg
     1501 taggcttcga tatcgtcggt cgggtcatac gtggacttgt cacgcttgct ccactccgtg
     1561 ccaccggact gtgtgatgtt gtttgccgca ctgcggccca tatccacctc aaccggatca
     1621 aaggcttcac cggtcatggt gtatttgccc ttaagcacgg cagaaactgc ctgcatctct
     1681 tcgacctggg caatggccag ctcttcgtca cgcatgttct gcatgatgat gcgacggcgg
     1741 cggtaagccg ggtccgccag attctgcgga tcttcatccg gcaggcgacg cagggtcatc
     1801 tgcggattca cctcatgctt cggcttgaca tatcccggcg taaattcaga ggtggagccg
     1861 ccacgggaac ggataacctc accggaaaca atcggcgaaa cgtacagcgc catgtttacc
     1921 agtcccggaa tttgtgagag atagactttc tccgtggtga agggatagct ctcacggaaa
     1981 aagagacgca gaaacagcgg atcaaactta aatttctgct catttgccgc cagcagctgg
     2041 gcggttgtgt acatcgacat aaaaaaatcc cgtaaaaaaa gccgcacagg cggcctttag
     2101 tgatgaaggg taaggttaaa cgatgctgat tgccgttccg gcaaacgcgg tccgtttttt
     2161 cgtctcgtcg ctggcagcct ccggccagag cacatcctca taacggaacg tgccggactt
     2221 gtagaacgtc agcgtggtgc tggtctggtc agcagcaacc gcaagaatgc caacggcagc
     2281 accgtcggtg gtgccatccc acgcaaccag cttacgggtg gcggtgtcca gcatcagcgg
     2341 ggtcattgca gacgctttcg cactcaatcc gccgggcgcg gttgcggtat gagccgggtc
     2401 actgttgccc agcggctggt aatgggtaaa ggtttctttg ctcgtcataa acatccctta
     2461 cactggtgtg ttcagcaaat cgttaacggc atcagatgcc gggttacctg cagccagcgg
     2521 tgccggtgcc ccctgcatca gacgatccag cgcagtatca ctgcgcgcct gtgcactctg
     2581 tggtgctgcg gccagaatgc ggcgggccgt ttccacggtc ataccggggg tttctgccag
     2641 cacgcgggcc tgttcttcgc gtccgtgagc ctcctcacag ttgaggatcc ccataatgcg
     2701 gctgttttct gccgcaaccg cagcggtgat ctgcgcgttc acgtccggct gcgccgcgct
     2761 ggcgttttcg ccctccatcg ctggcaccac gccagtaacg tcagcctgcg aagcagtggc
     2821 tgaaacagtt gttgattgag tctctttggt cattcgccct cctgagagac gggatttacg
     2881 tgcatccagt gcatcacgca taacggtgat cgcatcggtg ctgttgacaa gttcatcagc
     2941 cagtccggca tcaatggcct cctgaccgct gtacactgca gcctcggtat ccagcacagc
     3001 ctgcacggac aggccggtat atgccgacac cttctgcgca aacatccggc gggttgcatc
     3061 catccgggac tgcagtgttt cccggacatc acccggtaga tggctgtagg ggttgccatc
     3121 caccttatgg ctgccgctgt aaatcagcgt gatttccacg ccctgtttct ccagggcagc
     3181 accgtaatta ctgtgagcca tcatgacgcc gatggagcct gtccgggcgg tctgcgtgac
     3241 cagacgccgg gaggcggcgc tggcaagcag ctgacctgca ctgcagttca tgtcgttggc
     3301 cagcgcccat accggtttta tgtcacgcac acgggcgatg atgtcagcac agtcaaatgc
     3361 tcccgccacc attccgcccg gtgtgtccat atcgagcaga atgccgtcca ccatcgggtc
     3421 gctggcagcc tgttgcagac gggcgataat gccgttgtaa ccggtcatcc ccgaatacgg
     3481 ctgcagcgcc cgcgtccggc tgaccagcgt accggacacc ggcagcacgg cgatgccgtt
     3541 catgacctga taactccggg cctgtcgtgg tccgtcatca tcaccggata acgccagcgc
     3601 cgcgggtgcc tctccggcag tcaggctgtc gccggatact gcatccgtca ggcggctgat
     3661 cccaagctgg cctgcaagcg cacaaaagaa aacccgcgca taggcgggtt caagcatcag
     3721 cggctcatta aaagccatgc tggcaatatg cgggagatta cgcagctctg ctgtcactct
     3781 tctcctcctc tgttgattgt cgcagcccgg attcaaatgc cgcagccgcc caggcgggcg
     3841 gtttaagacc agctgc
//
LOCUS       DAZKIV010000255         3853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZKIV010000255 DAZKIV010000000
VERSION     DAZKIV010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.257"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(126..284)
                     /locus_tag="ACHOT6_004650"
     CDS             complement(126..284)
                     /locus_tag="ACHOT6_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HFP9949727.1"
                     /translation="MHEPPVQPDRCALSGNYRLESNPVRHDLTPLAASIGNWIRWIEI
                     LRVLEVVA"
     gene            complement(954..1145)
                     /locus_tag="ACHOT6_004651"
     CDS             complement(954..1145)
                     /locus_tag="ACHOT6_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HFP9949728.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
     gene            1210..1533
                     /locus_tag="ACHOT6_004652"
     CDS             1210..1533
                     /locus_tag="ACHOT6_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HFP9949729.1"
                     /translation="MLTIRVTDDEYARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREQR
                     VQDDS"
     gene            1523..1837
                     /locus_tag="ACHOT6_004653"
     CDS             1523..1837
                     /locus_tag="ACHOT6_004653"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949730.1"
                     /translation="MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELI
                     DATPFAKKYTSGVLSFAENELPPGGREKVMASFERVLMPGLEKISTASCGWSTRTRDG
                     LS"
     gene            1804..2172
                     /locus_tag="ACHOT6_004654"
                     /pseudo
     CDS             1804..2172
                     /locus_tag="ACHOT6_004654"
                     /inference="COORDINATES: protein motif:HMM:NF015397.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2210..2728
                     /locus_tag="ACHOT6_004655"
     CDS             2210..2728
                     /locus_tag="ACHOT6_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001447511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HFP9949731.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLNDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVEFVQDGNRKFLVLPYGKSAEVI
                     SFQGKEWVHLKE"
     gene            2735..2968
                     /locus_tag="ACHOT6_004656"
     CDS             2735..2968
                     /locus_tag="ACHOT6_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HFP9949732.1"
                     /translation="MTELETHLLNALEQLQQDYMQRLSEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENWR"
     gene            complement(3270..3737)
                     /locus_tag="ACHOT6_004657"
     CDS             complement(3270..3737)
                     /locus_tag="ACHOT6_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949733.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRVLQQLQIQEVCNSTLWETAKQQLLQESDGRYFAINSKHL
                     DFPGSGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFSHNLT"
BASE COUNT          945 a          856 c         1148 g          904 t
ORIGIN      
        1 aaaaaaacca ccgttatcaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttat ccagttacca
      181 atggatgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgagggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccagcggcg cagcctttcg ttgccctttg cctttttgct cacatgttct ttccggcctt
      601 atcccctgat tctgtggata accgtattac cgtctttgag tgagctgata ccgctcgccg
      661 cagccgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gccggaagct
      721 gcattttctc cttacgcatc tgtgcggcat ttcacatcgg ctatggtgca cttttcatac
      781 aatccgcact gatgccgcat aagtaagcca gtatacactc cgctatcgct acgtgactgg
      841 gtcatggctg cgccccgaca cccgctaaaa cccgctgacg cgccctgacg ggcttgtctg
      901 ctcctgacat ccgctcacag gcaagctgtg accgtctccg ggagctgctt gtgtcagagg
      961 ttttcaccgt catccccgaa gcgtgcgagg caactgcggt aaagctcgtc ggcgtggtcg
     1021 tggagcgatt cacagatatc agcctgttca tccgcgtcca gttcgttgag cttctccagc
     1081 agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgttta
     1141 gtcactgatg cctccgtgta agggggattt ctgttttatg gggtaatgat accgatgaat
     1201 cgcgagagga tgctcaccat acgggttact gatgatgaat atgcccggtt actggaacgc
     1261 tgtgagggta aacagctggc ggtatggatg cggcgggtct gcctgggtga gccggttgca
     1321 cgctccggga agctgccgac actggcaccg ccgttactgc gtcagctggc cgccattggg
     1381 aataacctga atcagacagc gaggaaggtg aacagcgggc agtggtcttc cggtgaccgg
     1441 gttcaggtgg tggccgcact gatggccatc gagagagagt tgcgcagctt acggcaggtg
     1501 gtcagggaac agagagtgca ggatgatagt taagtttcat gccaggggaa aaggtggtgg
     1561 cagtggtccg gttgattacc tgctgggaag ggagcgaaac cgggaagggg caacggtgct
     1621 tcagggtaat ccggaagaag tacgggaact catcgatgcc acgccatttg cgaagaaata
     1681 cacgtccggt gttctgtcgt tcgcggagaa tgagctgccg ccgggaggac gtgaaaaagt
     1741 gatggcgagc tttgagcgtg tactgatgcc cggtctcgaa aaaatcagta cagcatcctg
     1801 tgggtggagc accaggacaa gggacggctt gagctgaatt ttgtcattcc gaacatggag
     1861 ctacagaccg gaaaacgcct ccagccgtac tacgaccgcg cagacaggcc tagaattgat
     1921 gcctggcaga cgctggttaa tcaccattac gggctgcatg acccgaacgc cccggagaac
     1981 cgcaggacgc tgacactccc tgataatctg ccggaaacga aacaggcgct tgcagagagc
     2041 gtcacacgcg ggatagacgc tctttatcat gtcggggaga taaaaggccg tcaggatgtg
     2101 attcaggcgc tcacagaggc agggcttgaa gtggtcaggg tgacgcgaag cagtatcagc
     2161 attgcagacc cgaacggtgg gaagaatatc aggctgaaag gagcattcta tgagcaatct
     2221 tttacagacg ggcgcggagt tcgagaaaaa gctgaaagag agagccgaat ctacagagaa
     2281 aatgctgaac gacgagttca ggaagctcga agaatctgta agcaaggctg tgacatcaaa
     2341 cgagacgaaa atcaaagacg ctatagccct gttcacagct tcgacagagg aatcactgaa
     2401 aaaacaccgg gaaggggtga aagaggcgat gatgcagcac aggaaggacg tgttaaagct
     2461 ggcagggaat acgggcatga tgttactggg gatggtcatt ttcctgttta ccgtgagtgg
     2521 cgggacgctc tggtatcttg gcgggatgat acaggcgaac ctggaagaaa tcaggaagca
     2581 gaacgagagt ctggagaagc tgaacgcgaa gacatggggc gtggagtttg tgcaggacgg
     2641 gaacaggaaa tttctggtcc ttccttacgg gaagtcagca gaggtgattt cctttcaggg
     2701 gaaggaatgg gtacatctga aggagtgaca cagagtgaca gagctggaaa cacatttgct
     2761 gaacgcctta gagcagctgc aacaggacta tatgcagcgg ctgagcgaat gggagagcgc
     2821 cttcgtggaa ttgcagaaaa tgttttcgct tacgcaacgg gacaacgcga tgctgaacga
     2881 gcgggtcatg cagttgagtc agcaggtgca gcacttgagc gagcagacag aacgcttgag
     2941 ccagttatac agcgagaact ggagataaga gaggaacggc tgatacagga gcgcgaacat
     3001 gcgttatccc tggaacggga gcgtcagccg gaaatacagg aacgcacgct ggatggtcct
     3061 tcgctgggat ggtgaaacca tgaaaaatgg cagcttcagt ggattaggag gggttgatgt
     3121 ggcctgtacc atctggttgc ataggtattc atgcggttaa aatttatcag gcgcgatcgc
     3181 ggcagttttt cgggtggttt gttgccattt ttatctgtct gctgccgtga tcgcaccaaa
     3241 cgcgtttcag tggtgcgggc aataagatat tacgttaaat tgtgagagaa tggtccccat
     3301 atactgactt tatcatacgg atagggttgg tagtgggtcg attgggtttc ggcattgttt
     3361 ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga ttctcctgag
     3421 cctggaaagt ctaagtgttt agaattgatg gcaaaatatc ttccgtccga ttcttgaagt
     3481 agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat ttgtagttgt
     3541 tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg tattattctc
     3601 tcttttaata agctgtttac cacaggattt agttgttgtc taaagcacgc agataatgcc
     3661 attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg tgtttgatgt
     3721 gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac ggctataaaa
     3781 caaaattttc atagagtgat tcaagatctt cttgagatct ttttttgcgg tgcgtaatct
     3841 gctgcctgca aac
//
LOCUS       DAZKIV010000256         3853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZKIV010000256 DAZKIV010000000
VERSION     DAZKIV010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.258"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1732)
                     /locus_tag="ACHOT6_004658"
                     /pseudo
     CDS             complement(1..1732)
                     /locus_tag="ACHOT6_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1678..1899)
                     /locus_tag="ACHOT6_004659"
     CDS             complement(1678..1899)
                     /locus_tag="ACHOT6_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949734.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFYGEPEKTRNQDVT"
     gene            complement(1944..>3853)
                     /locus_tag="ACHOT6_004660"
     CDS             complement(1944..>3853)
                     /locus_tag="ACHOT6_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFP9949735.1"
                     /translation="TVKSFSEDERVITGIASTPSPDRDGDILEPEGAEFGSAIPFLWQ
                     HDHSRPVGQCTVRRVSEGLEITATLAKPVPDMPSQLAARLDEAWAAIKTGLVRGLSVG
                     FRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKSYDRQFSAASGNRKPVV
                     KIASSAGAAAQSTTVFHKEKTIMNIGEQIKSFENKRAALAASLEEVMTKAAEEGRTLD
                     VEEEEHYDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAAVASAPVIRVE
                     QKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTTTDPQW
                     AGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAAGWVGE
                     GKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVARLDTD
                     FVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVAANLQPTGAVWLMSST
                     NALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDIYLADDGGV
                     AVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRRRTAAVAVI
                     TGVNYGSASGG"
BASE COUNT          776 a         1259 c          974 g          844 t
ORIGIN      
        1 actgcctctg tcaccatctg ttcaagcatc ggacgcacat catccggggt gagactttta
       61 ccgtccgccg gctgtggaat atttgcgacc gcgtcattca ccgccttctg cagtacttcc
      121 ggatcgtagt cacgaccgtc acgcggaaca gggatatggc ttaccgcctc cttcaccatc
      181 tgctcaagca tcggacgcac atcatcgccc gtcacgcact tctgtaatac cacagacagg
      241 gaagccagtt tttcttcaaa cgtttgtgcc tgcgcggcta ttttcccctc aaatgtgcgc
      301 tgtaaatccg ccagcactgc ggaaaattct tcacccagcg cacggataat ggacagttca
      361 cgttcattca ttttttcaga atccccctga acatcgcctt caccgcatca tgctctgttt
      421 cagtgattgc cttattaccg tcagaggcag cgaccgcctg aggcacggaa gctgttttac
      481 cggacgacgc gaacggatct tcacgggcat cacggcggga caacgcttcc agactgtagt
      541 tctgctgctg aagatacagt gcatcaccgc cggccagggg cggcaggttc tcccgtttac
      601 gggcctcatt gggcgtgaga agcgtatttt tcaccgcatc ccccagcgtt ttcatgcgcc
      661 gctcactgtc cattctcagc agcgtggtga catcaaattc cgtactctcg ttttcccccg
      721 tttccagcgc ctcatccagt aacagttcaa tggactcaat cagcgtctgc aggcactggg
      781 aataatactg ctgctccagc gcctccacgt tgtcactgga aggcggttgt cccacgccaa
      841 tcttgtaggc cgggacacgg aacaccgaac agacaatttc agcggtcatc ttcagttgtt
      901 ccaccgtctg cgaatccacc ggtgaaaacg tcgtggggtt gtattttgcc ccgttgctca
      961 gaatggccgt tttccccgca ttttcgcctg tatacccgct gtcccagttg ctcttcagtt
     1021 ttttcgcatt ttcttccgta atactgccgg ggatctcaat caccccggac ggcctgccgc
     1081 catttctgaa aaaagacgtc gaattttcct gaatatgatg cccctgcgtg gccgccagcc
     1141 cggcggcata caccggcggc aaccctataa gcggatgaaa aaaacagtta aaccggtcgt
     1201 ggatcacttc ccgggcaggc accgtcaccg cctcagtgat cccgcagttc cggtccggcg
     1261 tgatgcggta gaacacctcg ccgtcatccg ccaccagagg ttcaacccgg ttccagtcca
     1321 gaatacgcag ttctttgatc tgcccccggg cattacggat tttcagcacc accgtattgc
     1381 cgtgacgcag tttggcgttc agccacagtt caaaaaactg gatgcggttc tgctgtgcat
     1441 tgggacgacg acagaggcgg gcaatatccc cccggcgcgt ttccctgcgt atcccctgcg
     1501 catccgtctg cataagacgc agccgcattt tggcgatatc ctgggatatc agcgaaatac
     1561 atgcaaacac cgcatgaaag gagaggacgg cttcaggatc ggctttcacg ccctgctgcc
     1621 aggcgccggc aaagggctca gccaccgcct gaaacaggct ggtccagccc gcctctctta
     1681 cgtcacgtcc tgatttctgg ttttttcggg ttcgccgtaa aaggttccac attcgccatg
     1741 ctccgcatca cgtttctttt tctgacctgc cggacgtcgc accgtgatgt actccgcctt
     1801 tcccaggcga accagcacct ccgcacacgg ctgtgccaca tcacggatat ccccggcccg
     1861 ggcatcatgc gtgccctgca gatatcggat ctttgccata acctgttacg ggaggcacac
     1921 gcctcccgtc ctccttatca gactcagccg ccggacgcac tgccatagtt cactccggtg
     1981 atcaccgcca ccgccgcggt acggcgacga cgccagttga tccagcgctc cgcacggatg
     2041 gccacgctgc ctgtctggaa catggaaacc agctccaccg gcgacggcgt ggtactgtcg
     2101 ccggtcggct cagactgcat ttccagtgat gcctcgcggg acatatccac tgccacgccg
     2161 ccgtcatccg ccagataaat atccggggca ttcaccagca ccagctggtc acccacgtac
     2221 tgggagacaa tcaccggcag cccctggaag gagccaccca gcagggtcat gtccgggtat
     2281 tctttctgac ccagcgcatt tttacgcatg gacagcgcca gagcattggt gctggacatc
     2341 agccagaccg caccggtggg ctgcaggttt gctgcaacaa actgtccaaa cgccgcctct
     2401 gcatccgcat ccgggttacc gcttgaggcc gtgcccttca catcatgggt gatggacgcc
     2461 ggggagacat ctgccaccgc cgcttttttc gggtccacaa agtctgtatc cagacgcgcc
     2521 accaccgctt ccgccagcgc attacggacc agtgcatcag cagccggact ggaaaaacgg
     2581 atcaattctt ccgtcagtac cgcaatggcc gacaccttcg catgactgaa ggtgatggat
     2641 tcaaaatcaa acttcgtcag gggttttgcc ttaccctcac ccacccagcc ggcagcaccg
     2701 cccgacacct gggcgtgcac acggatattg aacggcacct gacgaagtgc agggatcccg
     2761 ccctgaccaa atcgcccgat aatggtctga ggacgcaggt aatcaataaa gtcctgtgcg
     2821 tattcctgat attcagacag gctgcctgcc cactgcggat ccgtggtggt ccccgcgccc
     2881 actgccgatt tcaggacatg atgcagacga ctgtcatccg gatactgacg acgggccact
     2941 tccagggctt cagagcggac acctttagcc gcagccagcg atttggcaaa acgtgcgaaa
     3001 ccaatcccct tatccagttt ctgctccaca cggatcacag gcgcagaagc caccgcggcc
     3061 acattcccgt taccggcctg tttcaccggc tgcgccgtgg cggccttacc ggcttccagt
     3121 tcacgcaggc gcttcaggtg cgcatccacc tgacgtattt ccgctgcggt gttgtcgtaa
     3181 tgctcttcct cctccacatc cagcgtgcgc ccttcctctg cggctttggt catgacctcc
     3241 tcaagggagg ctgccagtgc tgcacgcttg ttttcaaaac ttttaatctg ttcgccaata
     3301 ttcattatgg tcttttcctt atgaaaaacg gttgttgact gtgccgcagc gccggcagaa
     3361 gatgcgattt tcaccaccgg tttccggttg ccggacgcgg cagaaaactg gcggtcgtaa
     3421 gatttaatgg tccggatggt gcattccgca ttcgcgggca cggtgacggc agacacctcc
     3481 atcagctccc agcgcagaaa atgcagtccg cctccgtcca gaaaggtgta ttcatgagga
     3541 cggaagccca cggacagccc cctgaccagc ccggtcttaa tggccgccca ggcctcatcc
     3601 agcctggcag ccagttgcga tggcatatcc ggcacgggct tcgccagtgt tgccgtgatt
     3661 tccagccctt cgctgacccg gcgcaccgta cactgcccca ccgggcggga atggtcatgc
     3721 tgccagagaa acgggatcgc actgccaaac tccgcgccct ccggctccag gatgtcacca
     3781 tcccgatccg gagaaggcgt tgacgcaatc ccggtgatca cccgctcatc ctcactgaag
     3841 gatttcaccg tca
//
LOCUS       DAZKIV010000257         3822 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZKIV010000257 DAZKIV010000000
VERSION     DAZKIV010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.259"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..776)
                     /locus_tag="ACHOT6_004661"
     CDS             complement(<1..776)
                     /locus_tag="ACHOT6_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFP9949736.1"
                     /translation="MNISNSQIDILRRDVRAGLRALFRPEPQTAVEWADASYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLSLAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFG"
     gene            complement(751..1296)
                     /locus_tag="ACHOT6_004662"
     CDS             complement(751..1296)
                     /locus_tag="ACHOT6_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9949737.1"
                     /translation="MKVNKKKLAEIFNVDPRTIERWQSQGLPCVSGGGKGVESVFDTA
                     TAIQWYAQREADIENEKLRKEVEDYRAASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSD"
     gene            1691..1876
                     /locus_tag="ACHOT6_004663"
     CDS             1691..1876
                     /locus_tag="ACHOT6_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9949738.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLSTESSGMNYIIK"
     gene            complement(2013..2150)
                     /locus_tag="ACHOT6_004664"
     CDS             complement(2013..2150)
                     /locus_tag="ACHOT6_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949739.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     F"
     gene            complement(2501..2968)
                     /locus_tag="ACHOT6_004665"
     CDS             complement(2501..2968)
                     /locus_tag="ACHOT6_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9949740.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     EQANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGRKRLRINATCPGS
                     VREAPTTSGVDNATGPQLADTVTRDYFTLRERLMTMHKQLEGAQDYIRTQCLK"
     gene            complement(3267..3800)
                     /locus_tag="ACHOT6_004666"
     CDS             complement(3267..3800)
                     /locus_tag="ACHOT6_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9949741.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVVPGMKLSKAKCAQVNAIERNKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
BASE COUNT         1016 a         1019 c          845 g          942 t
ORIGIN      
        1 ccaaacggcg tctctttgtc gccaaattta agatactgct cctccccgca gtgcgggcag
       61 gcaacatgaa aacgcataaa atgcggggat tcactggctg cacgctcaat ctggcaggtg
      121 cctctcactt tgggcgtgga gccacggatg gactttggcc agaccgagcc ttcaatacgc
      181 ttgtcaccca ggaacgtcgg agagccttcc tgttcaatat cctcatcaaa ggcagcaagt
      241 tcatcataac ccgccacatc aaccgacttt tcacggtagt tttttgccgc tttaccgccc
      301 aggcaccaga agccacgacc attggtgaaa cgcttcatag tgagcgtgtt atcccggtgc
      361 tttttgccat accacggggc cagagacagc agcgacggaa tatcgcggat ggtaggctcg
      421 acgtgggttt tcataaagtt ctcggcatca ccatccgtcg gcaaccagat aagggtgtta
      481 cgctgcttat gctctatgaa gtaagcataa acacccagca gcatttttga ataaccaaca
      541 cgggcagact tcaccacatt cacctcgcgg atgtagtcgc tgcccatcgc attcatgata
      601 gcccgctgaa agggtagtgt ttcccagcgc ccttcctggt atgcggattc tttcgggaga
      661 tagtaactgg catccgccca ttcaacggca gtctgtggct ccggcctgaa cagggctcgc
      721 agcccggcgc gtacatcacg ccgcagaata tcaatctgac tgttcgatat attcactcag
      781 caaccccggt atcagttcat ccagcgcggc tgctttgttc atggctttga taatatcccg
      841 tttcaggaaa tcaacatgtc ggttttccag ttccggaaaa cgccgctgta ctgagagagg
      901 gatcccgtca agaatactgg caatttcacc tgcgatccgt gacagcacga aagtacagaa
      961 tgcggtttcc accacttctg cggagtctct ggcattcttc agctcctggg cgtcagcctg
     1021 cgcacgcgta agtcgatggc gttcgtactc aatagtccca ggctggagat ctgcctcgct
     1081 ggcagccctg taatcctcaa cctctttacg gagtttttca ttttcgatat cagcttccct
     1141 ctgcgcatac cactgaattg ccgtggcggt atcaaataca gattcaacgc ccttaccacc
     1201 tccggagacg caagggagcc cctgagactg ccagcgttca atcgttcgcg gatccacgtt
     1261 gaaaatttcg gcaagtttct ttttattaac cttcatgaaa cagtctcaca acaaatacag
     1321 ggtccgacat gaaagtgccc gaaaatgact ttttcaggcg ttttcatgtc ggacctttac
     1381 ggattcgata ttagaaaaaa caaatagtta tgttcgagaa gtaccgacat gattttcccc
     1441 ggaaaatttt cataaataga gaaaacccgc gaggtcgccg ccccgtaacc tgtcggatcg
     1501 ccggaaagga cccacgaaaa tgataataat tatcatctac atgaggttta tcacgacatg
     1561 tgtgtacgcc atcaaaccac gagaaataat caattattac gcaggtatcg tattaattga
     1621 tctgcatcaa attagcgtaa aagcaacttc agataataca aatcagcaac actgaatacg
     1681 gggcaacatt atgtcatcaa agaacagaac ccgcagaaca acaacccgca acatccgatt
     1741 tccaaaccag atgattgaac aaattaacat cgctcttgat ctgaaaggtt caggaaactt
     1801 ttcagcgtgg gttattgaag cctgcagaag aaggctgtca acagagagtt cgggtatgaa
     1861 ttacataatt aagtaacatg gtgttcacag aacacgcagt taccggacac atcagttttc
     1921 cattcgctcc ccggcagtac aggcttcccc tctgacggga tagcctgaaa aaataacaca
     1981 gaaaattatt tgttataatt aatataactt actcaaaaaa aaagcgacga gaaaatcagc
     2041 atcaacgaac aataagcgcc aatacgtgat aacaaatggc agccatattt atctgcagta
     2101 taagcaatgg acaggataac cacaccagaa accgtcagca taaaatccat ttgaacttcc
     2161 ccggacaaaa tcgactcatc taaagattta cagctctttt tattatcaat atgttaaaag
     2221 taaaataaac agatgttcaa taacacgaat acaaaaacgt gctgaaattc aatgaatcca
     2281 tttctgtgtc atcaattaat agtgataaac atccggcttc ttccaccatc gcaccggaca
     2341 ggcgactatg aggggacaac gccgcgctcc gttaacgcgg taaaccccgg tgtgtatcgt
     2401 ttttgattat ccccgcacac tcgcgcagag gagtctccct gtcgggctgc ggtctctgtt
     2461 aatgagggaa tacagcgacg atacggcgca tcaacaaaac ttatttcagg cactgagtgc
     2521 ggatatagtc ctgtgcccct tccagttgct tgtgcatcgt catcagccgc tctctgaggg
     2581 tgaaataatc ccgtgtaacg gtgtctgcca gttgggggcc ggttgcatta tccacgccgg
     2641 aggtggtggg ggcttcacgc acggagcctg gacaggtggc gttgatccgc aggcgcttac
     2701 gaccagcggc aacgtcagcg cgaagagttt cattttcagc tctcgcatcg gctaattccc
     2761 tcgagtatct ggcatcaagt gcagcaacat cacgctggcg ctgctgcata tcagtaatgg
     2821 ttgcatttgc ctgctccagc tcactgactt ttttatcgcg ctgctctttg taggttatgg
     2881 cgttatcacg gtaatgattc agccccagac taagcgcacc acaggccacc agcagggcaa
     2941 tgatgaccac gcacagtacg cggttcattt caccaccagc gtatctgacc gatgaaataa
     3001 ccggaggcca taatcacaaa caccagccag ataagaatga acttccaggt ggataatttt
     3061 tcagccatct ctcgaatctc ccgaatcagt ttgctaaaat caaacacact ttctcctttg
     3121 acttttccag agtcaggaaa cacaaaaccc cgcttgcagc caacaaacgg ggtttttact
     3181 tttattcact tagtttttgt cagttcgcag gatttcgtgt tatccgtccg tgtgagcaaa
     3241 ccgcattttt cagcaaaata ttctgcttat ctgtcaattc cccagcacgc cagcgcactc
     3301 tcctggtcgc gacgggatac ctgaccgtaa cagttgtttg agcgaatacg gcagtctctg
     3361 ccaccgtcct taatccacca gcgaatcgcc tcacacgccc ccctgcgatc gcctgcatta
     3421 attcgtttat aaaacgtcga cgggaaacac ttaccggggc caatgttgta cggacagaat
     3481 gacgcaatcc ccgctttctg tggttcggtc aatggcactt tgatgttttt ctccacccat
     3541 gccagcgcct tattacgctc aatggcgtta acctgagcgc attttgcctt cgacaacttc
     3601 atgcctggaa caacaggttt accatccacc atgatggcac cacggcagat ggtccagatc
     3661 cccgcaccat cacggtatgc cgtggtgtgg ttaccttcct tttcatccag aaactggtcg
     3721 agaatgtcag gcgcaggcgc accagcggca atcagcgcca gaacggcagc cgacaggccg
     3781 tatttgattt tggtgttcat ggatatttat caggatttat cg
//
LOCUS       DAZKIV010000258         3730 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZKIV010000258 DAZKIV010000000
VERSION     DAZKIV010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.260"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..134)
                     /locus_tag="ACHOT6_004667"
     CDS             complement(<1..134)
                     /locus_tag="ACHOT6_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949742.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAAEWLDVTENAL
                     "
     gene            complement(178..378)
                     /gene="ymfT"
                     /locus_tag="ACHOT6_004668"
     CDS             complement(178..378)
                     /gene="ymfT"
                     /locus_tag="ACHOT6_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP9949743.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            469..1143
                     /gene="ymfK"
                     /locus_tag="ACHOT6_004669"
     CDS             469..1143
                     /gene="ymfK"
                     /locus_tag="ACHOT6_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9949744.1"
                     /translation="MKTVHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYIIKA"
     gene            1810..2172
                     /gene="yfdP"
                     /locus_tag="ACHOT6_004670"
     CDS             1810..2172
                     /gene="yfdP"
                     /locus_tag="ACHOT6_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFP9949745.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            2238..3062
                     /gene="yfdQ"
                     /locus_tag="ACHOT6_004671"
     CDS             2238..3062
                     /gene="yfdQ"
                     /locus_tag="ACHOT6_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFP9949746.1"
                     /translation="MSQNLDATAINQIHALISAQGVNEIISKIGADAVALPENFRIHD
                     LEKFNLNRFRFRGALSTASIDDFTRYSKDLADEGTRCFIDADNMRAVSVLNLGTIDEP
                     GHADNTATLKLKKTAPFSALLSVNGERNSQKSLAEWIEDWADYLVGFDANGGAIQATK
                     AAAAIRKITIEANQTADFEDNDFSGKRSLMESVEAKTKDIMPVAFEFKCVPFEGLKER
                     PFKLRLSIITGDRPVLVLRIIQLEAVQEDMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            3190..3714
                     /gene="yfdR"
                     /locus_tag="ACHOT6_004672"
     CDS             3190..3714
                     /gene="yfdR"
                     /locus_tag="ACHOT6_004672"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011076526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFP9949747.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFKELTES"
BASE COUNT         1026 a          871 c          867 g          966 t
ORIGIN      
        1 aataaagcgt tctctgttac atccagccac tcagcagctt cagcgtaacc ccccggcaac
       61 gccgcgatag tttttctgac agctttcacg taccactcag gctgtttttc tactttccag
      121 tgatgcttac ccacggttag cctcatcgtt ctgtggttaa aaattgaagg tgttctgtta
      181 atctttcgga tagatatccg gtcttaagtc agatttcgta attgcacctg acgtgcattg
      241 ctcaagtttt ttagccagca caaaactggc ttttttatag ccattgaaaa ccagccgtaa
      301 gtagccaggt gttgagccaa cttttccggc caactcgccc tgctgttctt tggttaaaga
      361 gtcccaatac gctttcatac aatatgtact tccggtatac atattacatg attgagatga
      421 accttcaaga tacttgtacc ttatcggtac aaaggtttta atttctttat gaaaacagtc
      481 catgacatcc ggcggtctaa cgccagaaaa ctgagagatg gtgttggcgg gaattcttcc
      541 tttgccacca tgattgatcg cgagccaacc cagaccagca ggtttatggg agatggtgct
      601 actaaaaata tcggtgacag catggcgcgg cacatcgaaa aatgtttcga cctgcctgtc
      661 ggatggcttg atcaagaaca ccagaccacg aacatcacaa aaaaacctga tgtttcaatc
      721 actaacaaac aaataacgtt agtccctgtc atatcatggg tacaggccgg agcatggaaa
      781 gaagttggct attctgaggt tgatttgagc acagcagaaa cttacccctg ccctgtaccc
      841 tgtggcgaaa tgacttatat cttgcgggtg attggtgatt caatgattga tgagtaccgc
      901 ccgggagaca tgatttttgt tgatcctgaa gtccctgcct gccacggtga cgacgttatt
      961 gcattgatgc acgatacagg cgaaaccacc ttcaagcggt tgatagaaga tggaacacag
     1021 cgttacctca aagcattaaa cccaaactgg cctgaacctt acattaagat caacggtaat
     1081 tgctctataa ttggtacagt gattttctca ggaaaaccaa gaagatacat aataaaggcc
     1141 taatcaatat ttataacctg cttcggcagg tttttttata cttgacaatg taccctcggg
     1201 atacataatg tatctaaaag aaacacaaca caggcaagat taaacaaaat ttggttgtaa
     1261 cacggcgtat ggcacatgcg tcgttagcgg tctggtgacg ttaaagggga taatccactc
     1321 cttgctcggg caaacaaacc aggtagccgg aatgtgcaag tcaatgagga tgctgataag
     1381 acgcctaacc agcgcggcga tccggtttga cgccagggaa gagaccaggg ggcaacgatg
     1441 agggcattta tggagccgcg acaaagtgtg gtgccgtaac tggctaagtg ctctcagcgt
     1501 tgtggtaatc cgcgaaatgg cgcggcggta agtatggcgg ggttactctt tccccgttga
     1561 ggacaccgga ttgtcaggtt gaccatacgc ctgagtgaca accccaccac aacagccact
     1621 gctttggcgg taccagtttg tacccttgct tccggctggt accgctcttt ttacaaaaca
     1681 gagaagagca tcaccggacg acgggctcat aacccaatcc atccgggcgg ctgccaccgc
     1741 aggtgttctt ctctgttttg tggagaaacc aaccgacctt gcagggtcga tatgatgagg
     1801 agcagcaaaa tggctagcga acgcagtact gatgtgcagg catttatcgg ggagctggac
     1861 ggcggcgtat ttgaaaccaa aatcggcgca gttctcagtg aagtcgcttc cggtgtgatg
     1921 aacacgaaaa ccaaaggtaa ggtctcactc aacctggaaa tcgaaccatt tgatgagaac
     1981 cgtgtgaaaa tcaaacacaa actctcatat gttcgcccga ctaaccgcgg gaaaatttcc
     2041 gaagaagaca ccaccgaaac gccgatgtat gtcaatcgcg gtggtcgcct gactattctg
     2101 caggaagacc agggacaatt actgactctt gccggtgaac ctgacggaaa actacgcgca
     2161 gcaggtcatt aatatcgttc ttaattaact gattatttat ctcatcactg aatatcttta
     2221 tatagtgagg acttattatg tctcagaact tagacgcaac cgcaattaat caaatccatg
     2281 cccttatttc tgctcagggt gttaatgaaa ttatcagtaa gattggtgcc gatgctgtgg
     2341 cattgcctga gaatttccgc attcatgatc tggaaaaatt taatttaaat cgcttccgtt
     2401 tccgtggtgc gctttccact gccagcatcg atgactttac ccgttattct aaagatcttg
     2461 cagatgaagg cacccgctgc tttatcgatg ctgataatat gcgtgccgtc agtgtgctta
     2521 acctgggtac tattgatgaa ccaggtcacg cagataacac cgccactctc aaactgaaaa
     2581 agacagcacc gttctctgcc ctgttgtctg ttaacggcga gcgtaactcc cagaaatcac
     2641 tggcagaatg gattgaagac tgggccgact accttgtggg ctttgatgct aatggtggcg
     2701 ccattcaggc aacaaaagcg gctgcggcaa tccgtaaaat cacgattgaa gcaaaccaga
     2761 ccgctgattt tgaagataat gacttcagcg gcaaacgctc cctgatggaa tctgtcgaag
     2821 cgaagaccaa agacattatg ccagtggcat ttgaatttaa atgcgttccg tttgaaggtc
     2881 tgaaagaacg tccgtttaaa ttacgcctca gcattatcac tggcgatcgt cctgtactgg
     2941 ttctgcgcat tattcagctg gaagcggtgc aggaagatat ggctaacgaa tttcgtgatc
     3001 tgcttgttga gaaattcaaa gacagcaaag tagaaacctt tattggtact ttcaccgcct
     3061 gatttcatta ctgcaaatgc ccctgcgggg gcatttatgg aaacgtaatt aactcaataa
     3121 tcaccggatg gtgagggctt ccttttaccc aaattcagcg cggtgcagcg catatacgtg
     3181 gagaacaaaa tgtcatttat taaaactttt tccgggaagc atttttatta tgacaagata
     3241 aataaagatg acatcgttat taacgatatc gcggtttccc tttcaaatat ctgtcgcttt
     3301 gccggtcatc tttctcactt ctacagcgtc gcccaacatg cggtgctttg cagccagctg
     3361 gtgccgcagg aatttgcttt tgaagcgtta atgcatgatg caacagaagc gtattgccag
     3421 gacattcccg caccactgaa acgccttctt cctgactata aacggatgga agaaaaaata
     3481 gacgccgtaa tccgtgagaa atacgggtta cccccggtta tgagtacgcc cgtgaaatat
     3541 gccgatctca tcatgctggc aaccgaacgc cgcgatctcg ggcttgatga tggctctttc
     3601 tggcctgtac tggaaggcat cccggcaaca gagatgttca acgtgattcc actggcaccg
     3661 ggccatgcct acgggatgtt tatggaacgc tttaaagagt taactgagtc gtaaaaatta
     3721 gcacgtacaa
//
LOCUS       DAZKIV010000259         3694 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZKIV010000259 DAZKIV010000000
VERSION     DAZKIV010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.261"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            267..700
                     /locus_tag="ACHOT6_004673"
                     /pseudo
     CDS             267..700
                     /locus_tag="ACHOT6_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237504.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            782..949
                     /locus_tag="ACHOT6_004674"
                     /pseudo
     CDS             782..949
                     /locus_tag="ACHOT6_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
     gene            1243..1719
                     /locus_tag="ACHOT6_004675"
     CDS             1243..1719
                     /locus_tag="ACHOT6_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949748.1"
                     /translation="MRRFVQQAVDHWPNLLAFHFTFHSTEGNIYGQQIHAFCISFYQQ
                     IHKRITARTHTTSPSSPLVLHWLLEQHGGAVIRCVLLLSQTSICHPRASVTVDEECSK
                     LVDLLQQIWPVISTGGQCQVERCFQVARGDTSEQYVALKTDALSFAVSDHKSSGAE"
     gene            1892..3119
                     /locus_tag="ACHOT6_004676"
                     /pseudo
     CDS             1892..3119
                     /locus_tag="ACHOT6_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          938 a          857 c          966 g          933 t
ORIGIN      
        1 ttgcagagca acatgtacga agtatgttta caggtaccag ccagatttgt accaggactg
       61 cggaggtgca ctcacttaat ccgaaatgcc cgctctgctc agggcgttca ggctgactat
      121 caatggcata cattttatcc ggtgtttcgc acccacaccg tgcaacgact cgacaccgga
      181 tatccgcagt cccgcttacc tgaataaaac ccgggggatt gattgtgtac tgtggtgtga
      241 aatcgcaccg tgatcgctga ttcgccgaat ccgcactgtg gtgtcaatca accacacgcg
      301 agagtcgtct caggcttcgc ctgcctcagg acgatttctg catagctaat tcccggggca
      361 acatttatag aggcgctgtg cgctctgatg gtcgccactt ctgacccgga ccatgctcct
      421 gatatgtcgg aatataatgt gatgttaggc atcagagagg aatatttcga tgcccctaat
      481 ctggggcgtt tgattctgga tgtctggagc aatccgggct ttgtgtcacc ggacgaatca
      541 aagcagtacc acccgggatg tggcgctgga tcgtcattca ccggactgtg aagacatact
      601 taaagagctg atatgggcgt tcagtgattt cgcccgcgat catcgtggtg tgtgtgatcc
      661 ggaagcccgt tacctcgccg gtaatccttg gtatccgtag caggccagct ctgaaagcac
      721 agctcagcag attccccgca ttcgcacaac cttcataaat ctcacagagg acattgtggc
      781 catgttgacc acaacaagcc acgacagcgt attgctgcgt tccgatgatc ccctgctcga
      841 catgaactac atcaccagtt tcaccggcat gaccgacaaa tagttttata aactgatcag
      901 tgaaggctat tttcaaactg ggcggtacaa aagcgaagtg gagcagtgga tgcagcagcg
      961 aatagaggga gcgtgggggc agcaggatga aacgtgttgt gatgccggta cgctggtatt
     1021 gtgcaaaatg ccggcgctac tattgtggaa atcagccctg tccctggtgc tggcgatact
     1081 ccctgcagac attctccggt cagccaatag gttgttgctc tttcctgatc gattcgtcat
     1141 tccattcttt ttgtttataa tgggcattac actggtgttg gcgtgtttac ctgcgtgtct
     1201 gcaccggttc agtaaaatta gaaaagttca gagtcgggga aaatccggcg ttttgttcag
     1261 caggcggtgg atcactggcc aaatctgttg gcatttcact tcacgttcca ttcgacagaa
     1321 ggaaacatct acgggcaaca gattcatgca ttctgcattt ccttttacca acagatacac
     1381 aaacgtatta ctgcacgtac tcacacgacc agtccatcat cgccgttggt gttacactgg
     1441 ctgctggaac aacatggagg agcagtaata cgatgcgtat tgctcctcag ccagacgagt
     1501 atttgtcatc cgcgagccag cgtcacggtt gatgaagaat gttcgaaact ggtggattta
     1561 ttgcaacaga tctggccagt gataagcact ggcggacaat gccaagtgga aaggtgtttt
     1621 caggttgccc ggggtgatac atcagagcaa tacgttgcgt taaaaacaga tgcgttgtcg
     1681 tttgctgtgt cggatcacaa atcatccggt gcagagtagc attctggctg tattctggta
     1741 aatcagcgta ttatcgatat attatcgtac tatcagaaac cgtatcgtct gttgatggct
     1801 agactggccc ccctgaatct tcagacaacc aatatcactt atttaagtga tagtcttact
     1861 actagttttt agactagtca ttggagaaca gatgattgat gtcttaggac cggagaaacg
     1921 cagacggtgt accactcaga aaaaatcgat attgttcagc aaagttttga accgggaatg
     1981 acagtctccc ttgttgcccg gcaacacggt gtggcagcca gccagttatt tctctggcgc
     2041 aagcaatact aggagggaag tcttactgct gtggccgcag gagagcaggt cgttcctgcc
     2101 tctgaacttg ctgctgccat gaagcagatt aaagaactcc agcgcctgct cggaaaaaaa
     2161 acgatggaaa atgaactcct taaagaagcc gttgaatatg ggcgtgcaaa aaagtggata
     2221 gcgcacgcgc ccttattgcc cggggatgag gagtaaacgt cgtcagtcgt tgtcgccggg
     2281 tgtcgcatga gcagttgcac atcattctca gaggaagtga tgactggaag gatggccgcc
     2341 gcagtcttca cactgatgat acggatgtgc ttctccggat acatcatatc atcggagagt
     2401 tgcccacgta tggttatcgt cgggtatggg cgctgcttcg cagacaggca gaacttgatg
     2461 gtatgtctgc gatcaatgcc aaacaagttt accggatcat gcgccagaat gcgctgttgc
     2521 ttgagcgaaa acccgctgta ccgccatcga aacaggcaca taccggcaga gtggccgtga
     2581 aagaaagtaa tcagcgatgg tgctctgacg gggtcgagtt ccgctgtgat aacggagaga
     2641 gactgcgtgt cacattcgcg ctggactgct gtgaccgtga ggcactgcac tgggcagtca
     2701 ctacgggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc
     2761 gcttcggcaa cgagcttctg gcgtctccag tagagtggct gacggataat ggttcatgct
     2821 accggcctaa tgaaacacgg cagtttgccc ggatgttggg gcttgaaccg aagaacacgg
     2881 cggtgcggag tccggagagt aacggcatag cagagagctt cgtgaaaacg ataaagcgtg
     2941 actacatcag tgtcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg
     3001 cattcgagca ttataacgaa aggcatccgc atagtgcact gggttatcgc tcgccacggg
     3061 aatatctacg gcagcaagcc agtaatgggt taagtgataa caggtgtctg gaaatatagg
     3121 ggcaaatcca gcgacaaaga acagggttaa tgaatctgta accagtccca ccttagcgct
     3181 atgcactttt gaatcacaga gcttgctaat cgtttgtccg actttaacag agttaatgac
     3241 ggcgatgatc tgatgccaag taaaacggac ttgatgtatg gcagatcctc tttcgccgaa
     3301 tggatatggg caatacttct taatacgaat ggtacggtta tgcgggaaga gactaacgcg
     3361 gttacttgtt cagctatttg tcccttgttc ctttttataa tgcacccgtt ccaggttagt
     3421 gctggctgta gcttatgtcc gggagacagc taatagatat atatactcgt catacttcaa
     3481 gttgcatgtg ctgcgactgc gttcgcttac cccaatcact tacttatgta agctcctggg
     3541 gattcactcg cttgccgcct tcctgtaact cgaattaagt agagtatagt gaaacggttc
     3601 agcttggttt ttattctgtt ttatttgttt atgcaatgag atctatctta taaagagaaa
     3661 cgcttaatta aatggaaatg caattattaa agtc
//
LOCUS       DAZKIV010000260         3645 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZKIV010000260 DAZKIV010000000
VERSION     DAZKIV010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.262"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..208)
                     /gene="escO"
                     /locus_tag="ACHOT6_004677"
     CDS             complement(<1..208)
                     /gene="escO"
                     /locus_tag="ACHOT6_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase activity
                     positive regulator EscO"
                     /protein_id="HFP9949749.1"
                     /translation="MAKINSQIKEVDGKLDDCEQSIKESIASKQAYCASLVNLDKVSL
                     YKYQIKNNAFDEQKQRLYEKKSSLS"
     gene            complement(268..1608)
                     /gene="escN"
                     /locus_tag="ACHOT6_004678"
     CDS             complement(268..1608)
                     /gene="escN"
                     /locus_tag="ACHOT6_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ATPase EscN"
                     /protein_id="HFP9949750.1"
                     /translation="MISEHDSVLEKYPRIQKVLNSTVPALSLNSSTRYEGKIINIGGT
                     IIKARLPKARIGAFYKIEPSQRLAEVIAIDEDEVFLLPFEHVSGMYCGQWLSYQGDEF
                     KIRVGDALLGRLVDGIGRPMETNIAAPYLPFERSLYAEPPDPLLRQVIDQPFILGVRA
                     IDGLLTCGIGQRIGIFAGSGVGKSTLLGMICNGASADIIVLALIGERGREVNEFLALL
                     PQSTLSKCVLVVTTSDRPALERMKAAFTATTIAEYFRDQGKNVLLMMDSVTRYARAAR
                     DVGLASGEPDVRGGFPPSVFSSLPKLLERAGPAPKGSITAIYTVLLESDNVNDPIGDE
                     VRSILDGHIVLTRELAEENHFPAIDIGLSASRVMHNVVTSEHLRAAAECKKLIATYKN
                     VELLIRIGEYTMGQDPEADKAIKNRKLIQNFIQQSTKDISSYEKTIESLFKVVA"
     gene            complement(1592..3619)
                     /gene="escV"
                     /locus_tag="ACHOT6_004679"
     CDS             complement(1592..3619)
                     /gene="escV"
                     /locus_tag="ACHOT6_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscV"
                     /protein_id="HFP9949751.1"
                     /translation="MNKLLNIFKKAESYHDLILALFFFMAVMMMIIPLPTVVVDIIIA
                     INISTALLLLMLSIYIKNPLELTSFPTILLITTLMRLSLSVSTTRLILLHHDAGDIIY
                     SFGNFVVGGNIVVGLVIFTIITIVQFMVITKGAERVAEVSARFSLDGMPGKQMSIDGD
                     MRAGVIDPLEAKVLRSRVQKESQFYGSMDGAMKFVKGDAIAGIIIVLVNLFGGVLIGM
                     WQFDMPFSEALSLFSVLSVGDALVAQIPALIISVTAGVVVTRVPGESEKEENLAGDIV
                     QQVSVNSRPFLISAALMLVMAIIPGFPTLVFLFLAVCLLGIAWKLQKKRTFGTGNNKD
                     AMGADLSNSQNISPGAEPLILNLSSNIYSSDITQQIEVMRWNFFEESGIPLPKIIVNP
                     VKNNDSAIEFLLYQESIYKDTLIDDTVYFEAGHAEISFEFVQEKLSTNSIVYKTNKTN
                     QQLAHLTGMDVYATTNDKITFLLKKLVLSNAKEFIGVQETRYLMDIMERKYNELVKEL
                     QRQLGLSKIVDILQRLVEENVSIRDLRTIFETLIFWSTKEKDVVILCEYVRIALRRHI
                     LGRYSVSGTLLNVWLIGSDIENELRESIRQTSSGSYLNISPERTEQIIGFLKNIMNPT
                     GNGVILTALDIRRYVKKMIEGSFPSVPVLSFQEVGNNIELKVLGTVNDFRA"
BASE COUNT         1108 a          853 c          670 g         1014 t
ORIGIN      
        1 tcgaaagtga agattttttc tcatacaatc gctgcttttg ctcatcaaat gcattgtttt
       61 taatctgata tttatacaac gacactttat ccaaattcac cagcgatgca caatatgcct
      121 gcttagaggc aatcgactct ttaatggatt gctcgcagtc atcgagttta ccatccacct
      181 cttttatctg agagttaatc ttagccattg attctctcaa tcggttcgct ctgcttttac
      241 gaatagataa aattctgtcc aacatactca ggcaaccact ttaaataggc tttcaatcgt
      301 tttttcgtaa ctactgatat ctttggtgct ttgttgtata aagttctgaa ttaatttcct
      361 attttttatt gccttatctg cttcaggatc ttgccccatc gtgtactcac caatacgaat
      421 aagcagctca acatttttat aagttgcaat aagctttttg cattctgccg ccgcacgcaa
      481 atgctccgat gtaacaacgt tatgcataac acgactggca cttaaaccaa tatcaattgc
      541 agggaaatgg ttttcctctg caagttctcg ggtaagtacg atgtgcccat caagaataga
      601 gcggacttca tcgccaatag gatcattaac attatcgctt tctaacaaca ccgtataaat
      661 cgcggttatt gaaccttttg gcgcaggccc cgctcgctct aataattttg gtaacgaaga
      721 aaaaacactc ggagggaatc ccccccttac atcaggttct cccgatgcaa gcccaacatc
      781 acgtgcggct cgggcataac gagttacaga atccatcatt aataatacat ttttaccttg
      841 atcgcggaag tactctgcaa tagtcgtcgc cgtaaatgcg gctttcatcc tttccagcgc
      901 ggggcggtct gacgttgtga cgaccagtac acatttagaa agcgtggatt gaggtaagag
      961 cgcgaggaat tcatttactt cgcgaccacg ttcgccgata agagcaagga caataatatc
     1021 tgcggatgcg ccattacaaa tcatccccaa aagcgtactt ttaccaacgc ctgaaccagc
     1081 aaaaataccg atacgctgcc caataccgca agttagtagc ccatcaattg ccctgacgcc
     1141 aagtataaat ggctgatcaa taacctgcct taataaagga tctggtggtt cagcatacaa
     1201 gctgcgttca aacggaagat agggggcagc aatattagtc tccattggtc tgcctatgcc
     1261 atcaacaagc cgcccaagta gtgcgtctcc aacacgaatc ttgaactcat ctccctgata
     1321 acttaaccac tgaccacagt acatgccgga aacatgctca aaaggcaaca agaaaacctc
     1381 atcttcatca atagcgatca cctcggctaa acgttgactg ggttctatct tataaaaggc
     1441 gccaatacgc gcttttggta aacgcgcctt aataatcgtc ccgccgatat tgataatctt
     1501 gccttcatat ctggtagacg aatttaatga taatgccggc acagtgctat tgagcacttt
     1561 ctgaatacgt gggtattttt ccaatacaga atcatgctct gaaatcattt accgttccta
     1621 atactttaag ttcgatatta ttcccaacct cctgaaaaga gagcacgggg acagacggga
     1681 acgaaccttc aatcattttc ttcacatagc gcctgatatc taaagcggtc agaatgacgc
     1741 cgttccccgt tggattcatg atatttttta agaagccaat tatctgctca gttcgctccg
     1801 gtgagatatt cagatacgaa cctgatgacg tttgtctgat cgactctcgt agctcatttt
     1861 caatatcaga gccaataagc caaacgttca gaagtgtacc gctaacgcta tagcgaccta
     1921 aaatatgccg acgcagggcg atacgaacgt attcgcacaa gataaccaca tccttttctt
     1981 ttgttgacca aaaaataagc gtctcaaaga tagttctcag gtctctaatt gagacatttt
     2041 cctctacgag acgttgtagg atgtcaacaa ttttgctcaa accaagctgg cgctgcagct
     2101 ctttcacaag ctcgttatat tttctctcca tgatgtccat caaataacgc gtttcttgta
     2161 cgccgatgaa ctctttggca ttagacaata caagtttctt tagcaaaaac gttatcttat
     2221 catttgttgt tgcataaaca tccatacctg taaggtgagc gagctgttga ttagtcttat
     2281 tcgtcttata tacgatagag ttagtcgaaa gcttttcctg gacaaattcg aatgatatct
     2341 ctgcatgccc agcctcaaaa tagacagtat catctataag agtatctttg tatattgact
     2401 cttgatagag caaaaattct attgcgctat cattattttt aaccggatta acaataatct
     2461 taggcaatgg aattccactt tcctcaaaga aattccaacg catgacctca atttgctgtg
     2521 taatgtctga actataaata ttactactta agtttaaaat caatggctca gcgcccggtg
     2581 agatgttttg gctattagac aaatcagctc ccatagcatc cttattattg ccagttccaa
     2641 atgttctttt cttctgtaat ttccaggcta tccccaacag gcaaaccgcc agaaataaga
     2701 agaccaatgt aggaaagccc ggaataatcg ccattacgag cattagtgcg gcactgatta
     2761 aaaaagggcg actattaaca gaaacctgtt gaacaatatc acctgcaagg ttttcttctt
     2821 tttcgctttc accgggcaca cgagtaacaa ccacgcccgc ggtgacagaa ataataagcg
     2881 cagggatctg ggcaaccaaa gcatcaccga cagacaatac agaaaacagg ctaagtgcct
     2941 cactaaatgg catgtcaaat tgccacatac caatgagcac gccaccaaaa aggttaacta
     3001 aaacaataat gataccagca atagcgtccc cttttacaaa cttcatcgcc ccgtccattg
     3061 aaccataaaa ctggctttcc ttttgcactc gggagcgaag aacttttgcc tcaagcgggt
     3121 caataacgcc tgcgcgcata tcaccatcaa tactcatttg ctttcctggc ataccatcaa
     3181 gggaaaaacg agcactcact tcagcaacac gctcggcccc ttttgtaata accataaatt
     3241 gaacaatggt gataatagta aaaataacca ggccaacaac aatattaccg ccaacaacaa
     3301 agttaccgaa ggagtaaata atgtcacccg catcgtgatg cagcagaata agtctggttg
     3361 tactaacact gagcgacagg cgcatcaacg tcgtaatcaa caggattgtg gggaaagaag
     3421 ttagttcaag aggatttttt atatagattg agagcattaa taaaagtaaa gctgtcgaaa
     3481 tattaatcgc gataatgata tccaccacaa ctgttggtag tggaataatc atcatcatta
     3541 cagccataaa gaagaagaga gccagaataa gatcgtgata tgactctgct tttttaaata
     3601 tatttaagag tttattcatg atgtcatcct gcgaacgcgc tcaat
//
LOCUS       DAZKIV010000261         3638 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZKIV010000261 DAZKIV010000000
VERSION     DAZKIV010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.263"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..2903
                     /gene="eaeA"
                     /locus_tag="ACHOT6_004680"
     CDS             87..2903
                     /gene="eaeA"
                     /locus_tag="ACHOT6_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin type alpha"
                     /protein_id="HFP9949752.1"
                     /translation="MITHGFYARTRHKHKLKKTFIMLTAGLGLFFYVNQNSFASGENY
                     FKLGSDSKLLTHNSYQNRLFYTLKTGETVADLSKSQDINLSTIWSLNKHLYSSESEMM
                     KAAPGQQIILPLKKLPFEYSALPLLGSVPLVAAGGVAGHTNKLTKISPDVTKSNMTDD
                     KAFNYAAQQAASLGSQLQSRSLNGDYAKDTALGIAGNQASSQLQAWLQHYGTAEVNLQ
                     SGNNFDGSSLDFLLPFYDSEKMLAFGQVGARYIDSRFTANLGAGQRFFLPANMLGYNI
                     FIDQDFSGDNTRLGIGGEYWRDYFKSSVNGYFRMSGWHESYNKKDYDERPANGFDIRF
                     NGYLPSYPALGAKLMYEQYYGDNVALFNSDKLQSNPGAATVGVNYTPIPLVTMGIDYR
                     HGTGNENDLLYSMQFRYQFDKPWSQQIEPQYVNELRTLSGSRYDLVQRNNNIILEYKK
                     QDILSLNIPHDINGTERSTQKIQLIVKSKYGLDRVVWDDSSLRSQGGQIQHSGSQSAQ
                     DYQAILPAYVQGGSNIYKVTARAYDRNGNSSNNVQLTITVLSNGQVVDQVGVTDFTAD
                     KTSAKADNADTITYTAVVKKNGVTQANVPVSFNIVSGTATLSAKSANTNSSGKVTVTL
                     KSDKPGQVVVSAKTAEMTSALNAHAVIFVDQTQASITEINADKKTAKANGSDAITYTV
                     KVMKDGKPLSAQDVTFTATLGTLSKSTEKTDANGYAKVTLTSKTTGKSLVSARISGSA
                     IDVKAPEVEFFTPLAIDDGNVEIVGTGIKGTLPTVWLQYGQVKLKASGGDGKYTWSSA
                     NTGIASVDSTGQVTLRDKGSTTITVVSGDKQTATYIIARPSSMIVSINERMTYNNAMS
                     SCQSLSGRLPSSQKELADVFDTWGAANKYEHYETRNAMISWIKQTDQDMRQGVASTYD
                     LVKKNPLTNKVDINKPNAYATCVK"
     gene            complement(3117..>3638)
                     /gene="escD"
                     /locus_tag="ACHOT6_004681"
     CDS             complement(3117..>3638)
                     /gene="escD"
                     /locus_tag="ACHOT6_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE inner membrane
                     ring protein EscD"
                     /protein_id="HFP9949753.1"
                     /translation="GNKPGFFLLSGYIPPSPKWSEVENLLLNTPGVAGWEIHNNSNNK
                     INELASEFKKNKLINYVNIFKKNDVIIVAGEVSQQNESKILAIINAMNKNSNVKILFQ
                     NIQPYISADIFPGKILRISGTMTNPTIALDNGTSLGIGSILKGGYVIDAIDPKDGINI
                     SRPDEYIHIPLSY"
BASE COUNT         1064 a          696 c          813 g         1065 t
ORIGIN      
        1 aataaacatt attacgccgg aagataaaac ttatttatta gtattaatta actaatcatt
       61 ctaactcatt gtggtggagc ccaaacatga ttactcatgg tttttatgcc cggacccggc
      121 acaagcataa gctaaaaaaa acatttatta tgcttactgc tggtttagga ttgttttttt
      181 atgttaatca gaattcattt gcaagtggtg aaaattattt taaattgggt tcggattcaa
      241 aactgttaac tcataatagc tatcagaatc gcctttttta tacgttgaaa acaggtgaaa
      301 ctgttgccga tctttctaaa tcgcaagata ttaatttatc gacgatttgg tcgttgaata
      361 agcatttata cagttctgaa agcgaaatga tgaaggctgc gcctggtcag cagatcattt
      421 tgccactcaa aaaacttccc tttgaataca gtgcactacc acttttaggt tcggtacctc
      481 ttgttgctgc aggcggtgtt gctggtcaca cgaataaact gactaaaata tccccggacg
      541 tgaccaaaag caacatgacc gatgacaagg cattcaatta tgcggcacaa caggcggcga
      601 gtctcggtag ccagcttcag tcgcgctctc tgaacggcga ttacgcgaaa gataccgctc
      661 ttggtatcgc tggtaaccag gcttcgtcac agttgcaggc ttggttacaa cattatggaa
      721 cggcagaggt taatctgcag agtggtaata actttgacgg tagttcactg gacttcttat
      781 taccgttcta tgattccgaa aaaatgctgg catttggtca ggtcggagcg cgttacattg
      841 actcccgctt tacggcaaat ttaggtgcgg gtcagcgttt tttccttcct gcaaacatgt
      901 tgggctataa catcttcatt gatcaggatt tttctggtga taatacccgt ttaggtattg
      961 gtggcgaata ctggcgagac tatttcaaaa gtagcgttaa cggctatttc cgcatgagcg
     1021 gctggcatga gtcatacaat aagaaagact atgatgagcg cccagcaaat ggcttcgata
     1081 tccgttttaa tggctatctg ccatcatacc cggcattagg tgccaagctg atgtatgagc
     1141 agtattatgg tgataatgtt gctttgttta attctgataa gctgcagtcg aatcctggtg
     1201 cggcgaccgt tggtgtaaac tatactccga ttcctctggt gacgatgggg atcgattacc
     1261 gtcatggtac gggtaatgaa aatgatctcc tttactcaat gcagttccgt tatcagtttg
     1321 ataaaccgtg gtctcagcaa attgagccac agtatgttaa cgagttaaga acattatcag
     1381 gcagccgtta cgatctggtt cagcgtaata acaatattat tctggagtac aaaaagcagg
     1441 atattctttc tctgaatatt ccgcatgata ttaatggtac tgaacgcagt acgcagaaga
     1501 ttcaattgat cgttaagagc aaatacggtc tggatcgtgt cgtctgggat gatagttcat
     1561 tacgtagcca gggcggccag attcagcata gcggaagcca aagcgcacaa gattaccagg
     1621 ctattttgcc tgcttatgtg caaggtggta gcaatattta taaagtgacg gctcgcgcct
     1681 atgaccgtaa tggcaatagc tctaacaatg tacagcttac tattaccgtt ctgtcgaatg
     1741 gtcaggtggt cgaccaggtt ggggtaacgg actttacggc tgataagact tcggctaaag
     1801 cggataacgc cgataccatt acctataccg cggtggttaa aaagaatggg gtgactcagg
     1861 ctaatgtccc tgtttcattt aatattgttt caggaactgc aactttaagt gccaagagtg
     1921 ccaatactaa tagtagtggt aaggtgactg taacgctgaa atcagataag ccgggtcagg
     1981 tagttgtgtc tgccaaaacc gcggagatga cttcagcact taatgcccat gcggttatat
     2041 ttgttgatca aactcaggcc agtattactg agattaatgc tgataaaaaa acagcgaagg
     2101 caaatggatc tgatgcgatt acctatactg ttaaagtgat gaaagacggt aagccgttat
     2161 ctgcccagga tgtgaccttt acggcaacct taggtacgtt aagtaagtcc actgaaaaaa
     2221 cggatgcgaa tggctatgct aaagtaacat taacatcgaa gactacagga aaatcgcttg
     2281 ttagcgctcg tattagcggg agcgcaattg atgtcaaagc acctgaagtt gaattcttca
     2341 ccccacttgc tattgatgac ggtaatgttg aaattgtagg aactggaatc aaagggacgt
     2401 tacctacggt atggttgcaa tatggacaag ttaagttgaa agccagtgga ggtgatggga
     2461 aatatacatg gagttcggca aatactggaa ttgcttcggt ggattccact ggtcaagtta
     2521 ccttaaggga taaggggagt acaactatta ccgttgtatc aggtgataag caaactgcaa
     2581 cttatataat tgcaagacct agcagtatga ttgtctctat aaacgagcgt atgacttata
     2641 ataacgcaat gagttcatgc cagagcttga gcgggaggtt gccatcttct caaaaagaac
     2701 tagctgatgt attcgataca tggggggcag caaataaata tgaacattat gagactcgta
     2761 acgccatgat atcgtggata aaacaaacag atcaggatat gaggcaggga gttgcaagta
     2821 catacgatct ggttaagaaa aatccgctta ctaataaagt agatataaat aagcctaatg
     2881 cttatgcaac ttgcgtaaaa taaactcaat aaacatctaa tagatgtccg gctaagatgt
     2941 atgttttagt cggacatttt tatgcgatat attctatatt agtacctttc gattagtatg
     3001 ctggtaagta atcctccagc caccttttct cttcgcccaa taagctgaca ttatggagac
     3061 catatctgca tccacacaat aaaaaaccct ccgaagaggg ggaagatgac aagacgttaa
     3121 tacgatagcg gaatatgtat atattcgtct gggcgggaga tgttgatacc atctttagga
     3181 tcaatcgcgt ctatgacata gcctcctttc agaatgctac caattcccag gcttgtaccg
     3241 ttatcaagag caattgttgg attcgtcatc gtgccgctga ttctgagtat ttttccagga
     3301 aagatatctg ctgaaatgta cggttgaata ttctgaaata gtatcttgac gttactattt
     3361 ttgttcattg catttataat ggcaaggatc ttactctcgt tctgttgaga aacttccccc
     3421 gcaacaatga tgacatcatt ctttttgaaa atattgacat agtttattaa tttattcttc
     3481 ttaaactctg atgccagttc attgatttta ttgttactgt tgttgtgtat ttcccatcca
     3541 gcaactcctg gcgtattcag taacagattt tcaacttcgc tccatttggg tgaaggggga
     3601 atgtatcccg ataacagaaa gaatccaggc ttattacc
//
LOCUS       DAZKIV010000262         3633 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZKIV010000262 DAZKIV010000000
VERSION     DAZKIV010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.264"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..458
                     /locus_tag="ACHOT6_004682"
     CDS             201..458
                     /locus_tag="ACHOT6_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949754.1"
                     /translation="MRIEELREIFSEDGLYTVRVEKGAIVSHCRIKCLQSQQRKSGAA
                     LIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            528..806
                     /locus_tag="ACHOT6_004683"
     CDS             528..806
                     /locus_tag="ACHOT6_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949755.1"
                     /translation="MAQNSRLHNSDNSAVFASRHGRRSHAFKSDWFRHAPCTEEQAEW
                     LIQNYRRRGYEFRKALSLDYRHWIIYVRLPYSERPPRPSRTFQQRIWR"
     gene            808..1864
                     /locus_tag="ACHOT6_004684"
                     /pseudo
     CDS             808..1864
                     /locus_tag="ACHOT6_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            1865..2229
                     /locus_tag="ACHOT6_004685"
                     /pseudo
     CDS             1865..2229
                     /locus_tag="ACHOT6_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
     gene            2226..2915
                     /locus_tag="ACHOT6_004686"
     CDS             2226..2915
                     /locus_tag="ACHOT6_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFP9949756.1"
                     /translation="MNNHYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKVTSIPLVSPVEFSTSSWRRAVLSLEEHHK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNTQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKQKA
                     AFSKQSVAKVD"
     gene            3111..3186
                     /locus_tag="ACHOT6_004687"
     tRNA            3111..3186
                     /locus_tag="ACHOT6_004687"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3144..3146,aa:Ile,seq:cat)
     gene            3277..3350
                     /locus_tag="ACHOT6_004688"
     tRNA            3277..3350
                     /locus_tag="ACHOT6_004688"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3311..3313,aa:Arg,seq:tct)
     gene            3528..>3633
                     /locus_tag="ACHOT6_004689"
     CDS             3528..>3633
                     /locus_tag="ACHOT6_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9949757.1"
                     /translation="MVFLVKKARRAATEIKKFEKRDLAQVVINAAYLVA"
BASE COUNT          917 a          848 c          986 g          882 t
ORIGIN      
        1 tccctcgcca cctctgatgt gtcaggcatc ctcaacgcac ccgcacttaa cccgcttcgg
       61 cgggtttttg tttttatttt caacgcgttt taagttccgg acggcgccgg aatagaatca
      121 aaaatactta agtagcgcgc agggagaaga gggatggacc ccgaacaggg gagtgctatt
      181 tatctggaag gattctgttg atgagaatcg aagaattacg tgaaattttt agtgaagatg
      241 gcctctatac tgtgcgcgtt gagaagggcg ctattgtcag ccactgccgt attaaatgtt
      301 tacagtctca acaaaggaag agtggagctg cgttaattca ttttgtggat gggcttgtga
      361 cggatggttt tattttgcgt gcaaatgaat ttgtcacatc gttgccgtct ctgaaagaag
      421 ctgggattaa ggctggtttt tctgcttttg aagattagtg aattcatcta caattcagcg
      481 cagggctgaa cccctgttga gtaacactgt gccaccggag aaagccgatg gcgcaaaatt
      541 ccagactaca caattctgat aattcagccg tctttgccag caggcacggg cggcgttctc
      601 atgcattcaa atctgactgg ttccggcacg ccccatgcac tgaagaacag gccgaatggc
      661 tgattcagaa ctaccgcaga cgtgggtatg agtttaggaa agccctcagc ctcgattatc
      721 gtcactggat aatctacgtc aggctgccgt actccgagcg cccaccgcgt ccgtcccgca
      781 cattccagca acgcatctgg aggtaacgtg cgggtattac ttcgacctgt tccggtaccg
      841 gaacttgggt tggtggtcct taaaccaggc cgtgaatcca tgcaggtatt ccataaccct
      901 cgagtgcttg tggagccgga accgaaaagc atgcgcggtc tgccgtccgg catcgtccct
      961 gccgttcgcc agccgctggc ggaggataaa tcattactgc cgtttttcag caatgagcgt
     1021 gtgattcgtg ctgttggtgg cgctggtgca ctgtctgact ggctgttgcg tcatgtcaaa
     1081 tcctgccagt ggcctcatgg tgactatcat cacagtgaaa ccgttattca ccgttatggt
     1141 accggcgcaa tggtgttgtg ctggcactgc gacaaccagc tgcgtgacca gacatccgaa
     1201 tcactcgagc aacttgctca tcaaaacctg tcagcatgga tgattgacgt catcggtcac
     1261 gcaataagcg gtacgcagga gcgtgaatta tctctggctg aattatcctg gtgggcggtc
     1321 cgcaatcagg tggcggacgc gctaccggaa gcggtattac gtcgttcgct ggggttgcgt
     1381 gcggaaaaaa tccgctcaat gtaccgtgaa agcgacatcg taccgggaga gcagaccgcc
     1441 accatcatac tgaagcagcg cacaaaaaaa tcttgcgccg ctgcctcacg cccaccagca
     1501 aaacccgcca caggaaaaga cggtggtcag cattgccgtt gatccggagt caccggctca
     1561 gtatctccag cgccagaaac cacaacggga agagatgcct gtatacacgc gctgggtaaa
     1621 aacgcagaaa tgcatgacgt gcggtaatca ggcagatgat ccgcatcaca tcattggtca
     1681 tggactggga gggatgggaa caaaggctga tgatttgttt gttattccgc tgtgccgtaa
     1741 atgtcataac gaactgcacg ccggggtaaa agattttgaa gaaaaacacg gcagccagct
     1801 gttgttgctg attcgttttt taatgcacgc gagaaattcg ggtgtcctga agtggaaagc
     1861 atgaatgact gaacggatag aatttgtttt gccttacccg ccgacggtga atacctactg
     1921 gcgacgtcat ggcaatacgt atttcatctc ggaagccgga aagcgttatc gccgtgatgt
     1981 ggcgctaatt gttcgccagc agcggctgaa attaaacctg tccggaaggc tggtgataaa
     2041 gattattgca gagccaccgg ataagcgccg tcgtgacctg gacaatatcc tgaaagcacc
     2101 actggatgtg ctgacgcatg ccggacttct catagacgac gagcagtttg atgaaatcaa
     2161 tattgtgcgc ggtcagctcg ttcctggtgg cggctgggga taaaaatcac agaactggag
     2221 tgcgcatgaa taaccactat ttacagtttg tgcgtgagca gctcattatc gccaccgctg
     2281 atttgagtgg ggcaacaaaa ggtcagcttg aagcctggca ggagaatgcc atgttcgata
     2341 cagggcgtta caggcgaaaa aaaatccggt accgcgatga agtgactgga aaaatgataa
     2401 cgcgggataa tccaccaatc ccgggaaagc aatcgctggc gaaggtgacg tcaattcctc
     2461 tggtcagtcc ggttgagttt tcgacatcat cgtggcggcg ggctgttctg tctcttgaag
     2521 aacatcataa agcctggttg ttgtggtgtt acagcgggag tatttgttgg gaatatcaga
     2581 tcgcgataac acagtgggcg tggaatgaat ttaatactca atccggtacc agaaaaattg
     2641 ctgggaaaac gcaggaacgc ctgaaaaaat taatctggct ggcggcgcag gcagtaaaag
     2701 cagaactttt tggtggggaa ggttatgaat atcaggagct ggcattactg gcgggagtga
     2761 caactaaaaa ctggtccaaa acatttactc gtcactgggt tgcaatgaaa cacatttttc
     2821 accgactgga tagtgaggct ttattatttg taatgagaac acgttcaaaa caaaaggcgg
     2881 cattttcaaa gcaaagtgtt gcaaaagtag attgaaaggc atatatttca tgcaaatctg
     2941 atattttgcc gattttgtac gtgatggcaa aagcaaacaa aacccgccca caagcgggtt
     3001 tttttgtgcc acttatctcg gatagacatg gtgaatgcgc tggtggagga gataagggtg
     3061 atttttgtat gcttgcaaca ttgatttcgt aacgttatta tcctgcgccc ggccctttag
     3121 ctcagtggtg agagcgagcg actcataatc gccaggtcgc tggttcaaat ccagcaaggg
     3181 ccaccaaccg ccactagcca tcaggaaaga gcgtcaaccc tttaagttga gtgtgcgagg
     3241 ttcgagtccc cggtggtggt ccattatcgg tattctgcgt tgttagctca gcaggacaga
     3301 gcaattgcct tctaagcaat cggtcactgg ttcgactcca gtacaacgcg ctacacttat
     3361 tttcccggct cgcttttgcg ggcctttttt gtatctgcgc cacgcccggc gcataccaac
     3421 cacagagcct ttcggggggg gagcttacgg agtggtcagt gtgactttct ctgtgggcag
     3481 atcgctcccg ggcgttggct cacccaccca aaggaacgtc acgatgtttg gtatttttgg
     3541 taaaaaaagc ccgcagagcg gcaacggaaa ttaaaaagtt tgaaaaacgc gatctggcac
     3601 aggtggtgat taacgccgcc tacctggtgg cct
//
LOCUS       DAZKIV010000263         3595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZKIV010000263 DAZKIV010000000
VERSION     DAZKIV010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.265"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..117)
                     /locus_tag="ACHOT6_004690"
                     /pseudo
     CDS             complement(1..117)
                     /locus_tag="ACHOT6_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(119..406)
                     /locus_tag="ACHOT6_004691"
     CDS             complement(119..406)
                     /locus_tag="ACHOT6_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFP9949758.1"
                     /translation="MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGKRERSDVYNLASERMR"
     gene            complement(403..654)
                     /locus_tag="ACHOT6_004692"
     CDS             complement(403..654)
                     /locus_tag="ACHOT6_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFP9949759.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            complement(828..1001)
                     /locus_tag="ACHOT6_004693"
     CDS             complement(828..1001)
                     /locus_tag="ACHOT6_004693"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949760.1"
                     /translation="MMEIKAQQFVTSTGRQVLTDNGQQGMGGVAEAIFANCAELDNDQ
                     LNEIIEWVRLYQR"
     gene            1372..2577
                     /locus_tag="ACHOT6_004694"
     CDS             1372..2577
                     /locus_tag="ACHOT6_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFP9949761.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSHSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            2574..3536
                     /locus_tag="ACHOT6_004695"
     CDS             2574..3536
                     /locus_tag="ACHOT6_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFP9949762.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDVSVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGAS
                     MTKEEIAQAESISPAKVTRAFQAASVPDEMISVFPIINDISLSDYQFLLKLSEKATNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
BASE COUNT         1006 a          802 c          810 g          977 t
ORIGIN      
        1 tgaatgaaac agcaggcgac catcacgcgg tcgtgtttcc agtgcattac gcaaagccct
       61 acacatcaac tcagcatcag cggttaatga gagggctgaa ccgataatcc gccgtgaatt
      121 atctcattct ttcactggca agattataaa cgtcactgcg ctcacgctta cccacagcta
      181 caacagcgat aattaattgt tcgtcaatca cctgatagac taggcgaaaa cctgacgcac
      241 gtaattttat tttgtagcag tcctttatcc cgcgcagttt tgcggaagga atatgcggat
      301 tgtcacagca cttttttagc tttttcgcaa actgttgctg aatagtcttg tccagttttt
      361 gccactcttt cagcgcatct tccctgaatt ttacctgata cctcataggt atttatctag
      421 gtctacatca tgcagcggtt ggttgctgcg ttcggctacc agtttaacca gctcctgatc
      481 gtccagggca tcgagcatct tttcgtacag ctctgcgggt acacagtaga aagcaggctg
      541 attacggttc aggatagcga tcgggaaacc atcaccggcg ctgactgtcg ccatcggatt
      601 tttcttcagc tcgctgacac tggcgcttgt atcactgaga ataatgttag gcataggcca
      661 cctttcaaga ctatttaacc agtctcaaag atactcctta tatgacctgt taacaagtct
      721 ttttgttgtt ttttatacag atggacagaa tcacctgaat gtccaggtag tgcgggaatt
      781 aaacaagggg cgtaaaaaag gccgcttgcg cagcctgatg atggcattca gcgctgatag
      841 agccgaaccc actcgatgat ctcgttaagc tggtcgttat ccagttccgc gcagttcgca
      901 aaaattgctt ccgccacgcc gcccatacct tgttgtccgt tgtcagtaag gacttgccgc
      961 cccgttgatg tgacaaactg ttgagccttg atttccatca tattatccct gtttgaacac
     1021 tttgccgtgg catcactata ccaggggaga tctcgcgctg ccagcagaac tggacggaat
     1081 aacctgaacg tccattctgg catggcatca atgaatgtcc gttctgtgcc agaggtgggt
     1141 gtcacctacc cgtatcctaa tataacactc tgacgcgccc aacggtcttg tctaacccct
     1201 cgcctgctca gagacaatgt ctaactatgc ggtttgatgt gaatggtgac ttgcaacgta
     1261 cgataattta atggcaatgc taaatatatt gtacctcttt catatttgtg tacaatttga
     1321 ttgaactttg tacgcaatat atgggcaggg aatctatctc agaggtaaac tatgaaacga
     1381 gattacggta gtgtcggtac catagcgctc agagcaagtg ctctacttca ggccatgagt
     1441 cgggatattg aggagcaaag aaaagaattc aatctgacag agtatcatca aacatatact
     1501 cgtaatgcgg tcgcaaaatt gccgaagctg agccgacgca tcgtagaact ggccgttaaa
     1561 gaaatggaag aaagtggcta tgaatttaat aaaaagcggg taggcaacgt cgagcagtat
     1621 gcattaacaa ttcagaacgt tattgatatt tacgctcacc gacagatacc aaaatatcgc
     1681 gatgttcata aagaacctta cgtaattttt gtcgtcaact tgaaaggtgg ggtttcaaaa
     1741 accgtcagta cagtaacgct ggcgcatgca ctacgagtac atccggattt gctgcgtcat
     1801 gaccttcgta tccttgtgat cgaccttgac ccgcaggcgt ccagcactat gtttttggac
     1861 catacccata gtattggtac agtccttgaa actgccgccc aagcaatgct aaataatctc
     1921 gatgctgaaa cactgcgaga agcggttatc cgacccacta tgattcctgg cgttgatgtc
     1981 atccctgctt ctattgatga cggcttcgta gcaagccaat gggagtcttt ggttgctgaa
     2041 caccttcctg gttttaagcc ttctgaagta ctcaggaaaa ccatcattga ccgtatcgct
     2101 gatgattatg attttgtttt tattgatact ggccctcatc ttgatccatt tcttctcaat
     2161 ggcctggctg cgagtgattt acttctcacc cctactcccc cggcgcaagt tgatttccat
     2221 tcaactctca aatatttgac tcgtttacct gaaatgctag aacgtttgga agaagaaggc
     2281 gttgagcccc gcctgagtgc gagcataggg tttatgtcca aaatgaccag taagcgcgat
     2341 cacgaaactt cccatagcct ggctcgtgaa gtgtatgcga gtaatattct tgactcatcg
     2401 cttccgagac tggacgggtt cgaacgatgt ggtgaatctt ttgacactat catcagtgct
     2461 aaccctgttt cgtatccagg aagtgcagag gcattgaaaa aagctcgtac cgaagcggaa
     2521 cgatttacta aagccgtttt tgaccgcatt gaatacattc ggggagcatc caaatgaaaa
     2581 aggttttagc tcgagggcga gttctcggca atagcaattc agagttcgct cgcatgcttg
     2641 aaggtgacgg agagatcaaa acgtttactc ttaagtctgg gaaacaagca aaattcatta
     2701 aaacggttgt attaagcgga gatatagaat ctaaaacgtt cgttgatgta tctgttaatg
     2761 gacgcgatca gactatgctt tcgcgagagt cagttagtga cataactcgc acgattaaac
     2821 ttcagcaatt ttttccggct attggtcggg aagtagatgg acgaattgaa gtattagacg
     2881 gtacgcgtcg tcgtgctgcc tgtatctata acaacgtaaa attcgaaata ctggttacaa
     2941 aagacagtat tgatattgcc gacgcgagac aacttgcaaa agatatacag acagccagag
     3001 agcatagcct ccgagaacta ggaaaacgcc ttgaagttac ttatggggca agtatgacaa
     3061 aagaagagat cgctcaggct gaaagcatct ctccggcaaa agtaacacgc gcatttcagg
     3121 cggcatcagt accggatgaa atgatctctg tatttccgat aatcaatgat atttcgctgt
     3181 cggactatca atttttgttg aaactgtctg aaaaggcaac caacaaaaaa atgtcagtgg
     3241 aagagtttat ggatagagtt cgccaaaaac tgatggcttt acctgactat ccaacaattg
     3301 agaaaagcaa aatcctggcg gtcttcaggt ctgaaggaaa attgcttaca gaccggcctg
     3361 taaaaacagt ggaaactgag aagttgaaag agttttctga tcgtaaccaa tttgccagaa
     3421 agaagactga ccccagaaag cgtcttgttg tttatgagtt ctcccgcata tctaatgagg
     3481 tgcagttaga aatcgatgag gctataaaaa acattctaag aaaactgcct gaataggata
     3541 tgccgggtta atatcaggat gccaaatggc atcctttttt gcaattttca ccacg
//
LOCUS       DAZKIV010000264         3592 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZKIV010000264 DAZKIV010000000
VERSION     DAZKIV010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3592
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.266"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(97..173)
                     /locus_tag="ACHOT6_004696"
     tRNA            complement(97..173)
                     /locus_tag="ACHOT6_004696"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(137..139),aa:Arg,seq:tct)
     gene            complement(274..349)
                     /locus_tag="ACHOT6_004697"
     tRNA            complement(274..349)
                     /locus_tag="ACHOT6_004697"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(314..316),aa:Ile,seq:cat)
     gene            complement(378..1091)
                     /locus_tag="ACHOT6_004698"
     CDS             complement(378..1091)
                     /locus_tag="ACHOT6_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949763.1"
                     /translation="MVVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALITSQGFSGE
                     VTDMFLMMACLSLFETDERMSLFLSGCLSSISAKVRAIIQTDISASWTLGAIALQLHM
                     SESLLKTKLKNEGGMFSRLLLEERMRVAVNMLCSRYGYGQAVAEKCGYSSRSYFISVF
                     HRYYGFPPDRYVSRQGLDY"
     gene            complement(1229..1426)
                     /locus_tag="ACHOT6_004699"
     CDS             complement(1229..1426)
                     /locus_tag="ACHOT6_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9949764.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(1713..2531)
                     /locus_tag="ACHOT6_004700"
     CDS             complement(1713..2531)
                     /locus_tag="ACHOT6_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFP9949765.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTTPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(2683..3054)
                     /locus_tag="ACHOT6_004701"
     CDS             complement(2683..3054)
                     /locus_tag="ACHOT6_004701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFP9949766.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(3044..3415)
                     /locus_tag="ACHOT6_004702"
     CDS             complement(3044..3415)
                     /locus_tag="ACHOT6_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9949767.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(3428..>3592)
                     /locus_tag="ACHOT6_004703"
     CDS             complement(3428..>3592)
                     /locus_tag="ACHOT6_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9949768.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT         1147 a          905 c          669 g          871 t
ORIGIN      
        1 ccgtaagctc acccccgaaa ggctctgtgg ttgatatgcg ccgggcgtgg cgcagataca
       61 aaaaaggccc gccgaagcga gcctggaaaa taagtgtggc gcgttgtagt ggagtcgaac
      121 cactgaccga ttgcttagaa ggcaattgct ctgtccggct gagctaacaa cgcagaatac
      181 cgataatgga ccgccatcgg ggacccggcc ccgcaccaac aaccctgtta tggtgtcgtc
      241 tgctcttcct gataagctaa tggcggtttg tgatggtggc ccttgctgga tttgaaccag
      301 cgacctggcg attatgagtc gctcgctctc accactgagc taaagggccg agccaaaaaa
      361 taataatcag atgaaaatca ataatcaagc ccttgcctgg atacatatct gtctggcggg
      421 aagccataat agcggtgaaa tacagaaata aagtaggacc ggcttgaata accacatttt
      481 tctgctacag cctgtccata tccataccgg gaacataaca tatttacagc aacacgcatc
      541 cgctcttcca gcaacaagcg actgaacatg cccccttcat ttttcagttt tgtctttaac
      601 aaactctcac tcatatgcaa ctgtagagca atcgcaccaa gcgtccagct tgctgatata
      661 tctgtctgaa ttatcgccct gactttggca cttatgctgg ataaacatcc acttaaaaac
      721 aatgacatcc gttcatctgt ttcaaacaga gacaggcagg ccatcataag aaacatatcc
      781 gtgacctctc cggaaaatcc ctggctggta attaaagccg cagccaacgc aggattgttg
      841 ggttccagca acaggtaaag cggaatgtca gtcagacgag tcctcgtcag cttatgctga
      901 ctttccagat attgacttac gacggattcg cttatatcga caattttaac tttgccataa
      961 tgcataagga aaagctccct gatgcatttg gtggccagaa cgactgagcc tggcttaagt
     1021 gacaacgtat ccttttcaag aaaaatatta attggggagc aaaccatgat aactgaacag
     1081 acaacaacca ttataatttt actttcatta gcaattggtt agttcaatta tagccccaaa
     1141 aggtaaatta tcatcaacac ataagcaaag gactgacagg tgccgctaac acccaccagc
     1201 cgcccattta ccacaaataa aaaagccttc aggactgaag gcgtctgtaa caaccgcact
     1261 gatagtctgc cagacccgcc ataacaagct gggtcagtat taactggcag cgttcgcgtg
     1321 aaaggtaagt attctgcgca atctccccga ctgtcgccgg ttcggtgacg cttaattcat
     1381 caaacacaac tctggcggtt tctgtcatat cctgctgttt cagcatgtct ttttaccctt
     1441 tccggttaac gtgacacacc aataactctt gccgaaaaag ccagcaagct gaaagacctg
     1501 tattaataac taccagcaca tttaacgcac tgcgctactt tgcgggcaca aaaaacccgc
     1561 tcagaggcgg ggtcaagcta tgcggcgaaa taaccactct taacagcata cctgattttt
     1621 tacgtacgta aatgctttgc cgtgcatatt tttcatgcaa atgtcacgtc ctgctatttt
     1681 tcagtcttat aaatttaaaa ccatagaaaa aatcaattat gttttaaaaa tggataggta
     1741 aagaataaca agtgacacag attcaaacca aaatggaaaa gggtggcaac ccacaaacgc
     1801 ccactccaca tccatgacaa tccatacaca acaccagata acgtggcaaa taatacaagt
     1861 aaagcaccac ctgaatagtg ataaaaacca aacaacaaag ccgccacaat taatgcaacc
     1921 aatggagtcg ttacttctga tagccgtgat tgaatatacc ctctaaataa tgattcctct
     1981 gccagagaca caaaaaacaa attagccaat ataaactctg gcaaccactc aggaaaatga
     2041 atctctggct ttaatccacc aaaaaaaaca gccaaaaaca ggataagagg aacagagagc
     2101 gacagagccc cccacttcca caaagacact tctgatttta cttctttttt aaacaaagaa
     2161 gatgtacaca ggactaacaa aaatggcacc agcgctttat caaaattaaa atacattgta
     2221 tagggagtac tttgagggcc aacagtaaca gaatttagca ctacaggatt gtgaaaccct
     2281 ggccatagat ggaaagataa tgctatggct gataaaacta tgccaacttc atatatagat
     2341 ttagcccagg cgttatattt ccagttgaac ttcaaaataa taaaaaaaac gattgtagca
     2401 acaaaaaata acaccgacca atcaataata tcattaagca cagccaggac aacagacacc
     2461 gtcaacaatg aaaaagccac taccttatgc caactgagag ttgacagtga cagtactaac
     2521 acaatccaca taagcactcc ttttatttaa tgatgaagat tgattatcaa tattttcaat
     2581 tcaccaggca acattttatc taccttccac aatacatgac catcaaaaaa taaacatgtt
     2641 aattataaac acagaaaaca taaccctcat cactatatat ccctaccgca tatccatatc
     2701 taaccggaca ttcagagcca taagcattcc ttcaataata ccttccgctt tataaagcct
     2761 tttaccaata agcccatcag aacatctatg cttacgtgca agagccataa atgtcattcc
     2821 acctacgtaa taatccacca acaaatcgtg caaatactga ttattcttgt ttaatcgtgc
     2881 catacaacca caaattatca tggcgtcatc atcagaacac tgaggacgtg atttcacttt
     2941 agtcgggatt aatcctttaa aaccagcagc tatcgagggc catgttacat cttcatgatt
     3001 atttgctgcc catgcccccc atcgctcaag aaccatctgg atatcacgcg ccatcgttac
     3061 cacctctgat ttcgtaaatc ttcacgccca gccgtccacc tggcacaggc tgaccgcgca
     3121 caatattgat ttcatcaaac tgctcgtcat caatgagcac ttccgcatgc gtcagcgcat
     3181 ccagcggtgc tttcagaatg ttgtccaggt cacggcggcg cttatccggt ggttctgcaa
     3241 taatttttat tgccagccgt ccggacaggc ttaatttcag ccgctgctgg cgaacaataa
     3301 gcgccacagc ccggcgataa cgctcaccgg cttttgatac aaaatatgtg ctgccacgac
     3361 gtcgccagta ggtgttcacc gtcggcgggt aaggcaaaac aaactctata cgcatcagta
     3421 acctctttta cccgagcacg ccggttgcaa aagcgtgatc aagaaaacga aaaattaaat
     3481 caacctggga accatgcttt tcttcgaacg ccagcggatc cgcatgaagc tcgttgtgat
     3541 gctcccgaca cagcggtagc gtgaaaatat cgtgggattt tgttcccatt cc
//
LOCUS       DAZKIV010000265         3549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZKIV010000265 DAZKIV010000000
VERSION     DAZKIV010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.267"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..807
                     /locus_tag="ACHOT6_004704"
     CDS             <1..807
                     /locus_tag="ACHOT6_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9949769.1"
                     /translation="LISAEGATEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEES
                     GLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDPLGLDLLSLPSPRQK
                     PVSQSCTIDPITQQPVGRFIGDSKGNIVEPVGGRTIPYPPSNPGSADTHTLYQNNSNA
                     YRLNPIGHANNSTPHAHAHLPGTGSGMKGQGPSLDVNGRVVPWNSSAAHIGIRSLGTL
                     SFFSSVLHMFTLRSCKEGTFGQCYCAMYVEMSTGSNQDEADIMCGMPEYF"
     gene            822..1223
                     /locus_tag="ACHOT6_004705"
     CDS             822..1223
                     /locus_tag="ACHOT6_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6258 family protein"
                     /protein_id="HFP9949770.1"
                     /translation="MFDRIYLGDRAIKKIEFDLWSKQIRIQVNLISRIAYGTTEWSFY
                     DNEDLEDGYLVFSDVSFFNITPNGSIPDDYIISIITDNVNVEYFESTIVAVGQIPNTN
                     SGDIDNIGECQIFIRYKHSWIENKFKEKIIE"
     gene            1340..2113
                     /locus_tag="ACHOT6_004706"
                     /pseudo
     CDS             1340..2113
                     /locus_tag="ACHOT6_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            2125..2586
                     /gene="yibG"
                     /locus_tag="ACHOT6_004707"
     CDS             2125..2586
                     /gene="yibG"
                     /locus_tag="ACHOT6_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFP9949771.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            3221..>3549
                     /locus_tag="ACHOT6_004708"
     CDS             3221..>3549
                     /locus_tag="ACHOT6_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFP9949772.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLK"
BASE COUNT         1148 a          615 c          781 g         1005 t
ORIGIN      
        1 cttatcagtg cggaaggggc aacagaatgg tgcgcagaat acgatgaatg gggcaacctg
       61 ctgaatgaag agaacccgca tcagctgcag cagcttatcc gcctgccggg gcagcagtat
      121 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcga
      181 tatatcactc aggatccgat tggactgaag gggggatgga atttttatca gtatccgttg
      241 aatccggttc agtatataga ccctttggga ttagatctat tatcattacc ttctcccaga
      301 caaaaacctg taagtcaatc ctgtactatt gatcctataa ctcaacaacc tgttggtagg
      361 tttataggtg attctaaagg caatattgtt gaaccggtag gtggcagaac aatcccgtat
      421 ccccccagta atccaggaag tgcagatact cacactcttt atcagaataa ttcaaatgct
      481 tataggctta atccaatagg gcatgcaaac aattcaacac cacatgcaca tgcacattta
      541 ccaggaacag gtagcggtat gaaaggccaa ggtccatccc ttgatgttaa tggtcgcgtt
      601 gtaccatgga atagtagtgc cgcacatatc ggtattagat cactcggtac actaagcttt
      661 tttagttctg ttttacatat gtttacactt agatcatgta aagagggaac gtttggacaa
      721 tgttattgtg ctatgtatgt tgaaatgtca acgggaagca atcaagatga ggctgatata
      781 atgtgcggaa tgcctgagta tttttagaat agggggggaa tatgtttgat agaatatatc
      841 ttggtgatcg agcaataaaa aaaattgaat ttgatttatg gagtaaacaa attagaattc
      901 aggttaattt aatctcaagg atagcctatg gtacaacaga atggagtttt tatgataatg
      961 aagacttaga agatggttat ttggtatttt cagatgttag tttcttcaat ataactccta
     1021 atggctctat tccagatgat tatataattt caataattac agataatgtc aatgttgaat
     1081 attttgaatc aactatagtt gcagttggac aaattccaaa tacaaattct ggagacattg
     1141 ataatattgg tgagtgtcaa attttcatca ggtataaaca cagctggatt gagaataaat
     1201 tcaaagaaaa aataattgaa taaccatgtt atgtatagac attttagatg gaacgaacca
     1261 gaggaaggca tgcctccgac ttgggttata tgggcacgga gttttagtgt agtgaactca
     1321 acgacgcaaa ccgaataacc tgccgctgga gcttatcagc acggaagggg caacagaatg
     1381 gtgcgcagaa tacgatgagt ggggcaacct gctgaatgaa gagaacccgc accagctgca
     1441 gcagcttatc cgtttgccgg ggcagcagta tgatgaggag tccggcctgt attacaaccg
     1501 ccaccgctat tatgacccgc tgcaggggcg atatatcact caggacccaa ttggactgaa
     1561 ggggggatgg aatttttatc agtatccgtt gaatccggtc ataaatgtag atccgcaagg
     1621 tttggttgat ataaatttat accccgaaag tgatcttatc cattctgtag ctgatgagat
     1681 taatatccca ggcgttttca caatcggggg gcatggtacc cccacatcta ttgaatccgc
     1741 aacgcgcagt atcatgacag ctaaagatct agcatatcta attaaatttg atgggaatta
     1801 taaagatggg atgacagttt ggttattttc ttgtaataca ggtaaaggac aaaattcatt
     1861 tgctagccaa ttagctaaag agttacatac aaatgtaata ggacctgaca cgctatggac
     1921 gtggtggggg cgaggaacta atggtaagtt aaaaatggat acagtgctaa cagcaccaac
     1981 gaaccttaat tcaaataagg atctaatggc tataacaaca aaagaccttg gtaattggat
     2041 aacatatggg ccatctgggc accccatttc taatatgcaa ggtacgccag aaaaacccag
     2101 tgatataaga taggttgtag atgtatgaaa gcatgcttgt tactattttt ttatttttct
     2161 cttattggtc aattgcatgg tgctgatgtg aaaataaaag aaaacgaaag tgtgatggga
     2221 tctacagcaa tgacctatga tttaagtgaa gaaaagctga tgaaactgaa gtataaatca
     2281 caacatggtg attcagaggc atcatttcga ctatatcaat attactgctt tactaaaaat
     2341 aatattgata aacaattgcg attcttggaa agatcagcat ctcaggggaa tgttacagcg
     2401 cagtttaatt atggggtctt tttatcagat acaaatccaa cattatcaga atattataat
     2461 ttgaatagag ctatttattg gatggaattc gctgtaaata acggtaatat tgatgcaaag
     2521 agcaaacttc aagagctcaa aaagctaaag cgaatggata gaaggaagaa taaggagaat
     2581 ccgtaaaagc aatagttcat cagactgccg cagcagtagt atgatgatga atcagggctg
     2641 tactataact attatcggta acattaaccg cagcaggggc ggtataacac tcaggctccg
     2701 attgggctgg aaggtaggtg gaatttttat cagtatccgt tgaacccgat ttctggaatc
     2761 gatccccttg ggattagcca cctgtttata cagcataact ttaggtatgc tttcgtgtgt
     2821 ttcagatata ccatgtgatg ataattttta tgatgtgctt agtattccag tcgcttctgg
     2881 taataatatg caatgtaaaa ataatccccg ttgtacacat ttataaatag atggccaata
     2941 cctcaaggcc tatggcgttg gaatgttaat gacccgggag caagcaatag aaaacctgac
     3001 ggcatacgat tagtaccgtc agttaataca ggaacttata atcgcaatgg atttccaata
     3061 catagttgtt taaacgcttt tggtccgtct ttaggacctc gattctgttc cgagggttgt
     3121 ataactggtt tatctaatga catgcaaaaa ttaaatgagt taatattttc agagccagat
     3181 agcacattaa cagttacgga ctaaaaaata tggcaaggtt attaacaatt gtattttttt
     3241 ctctatttct gcgagtgcag ttactgcaag acaatttcag agagagttag atttggcaga
     3301 caaagcaata ttatgggcac taatatcagc cagtactaaa gagggacgaa aagcgtgttc
     3361 actaagctat tttgcctgta aggccgcaga ggctgaactc ggactggcat atatggctgc
     3421 gaatgataac aaggaatttc tcacatcctt atctaatata atgaggtaca aaatagatgc
     3481 tggactttcc gaatcctata catgctattt attaagtaag ggaaagatca taagaccata
     3541 tctgaaaaa
//
LOCUS       DAZKIV010000266         3515 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZKIV010000266 DAZKIV010000000
VERSION     DAZKIV010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.268"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..709
                     /locus_tag="ACHOT6_004709"
     CDS             32..709
                     /locus_tag="ACHOT6_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949773.1"
                     /translation="MKLPVKLLMSLMSLVSVIARAGEYKNYSRDEIKYWRYTSYKGWK
                     LPEGFTDEIFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCKPGA
                     WAEQPGACPTTYELYEGDATYRAAIDKALTPVGLNGMFGEDGYMDGPDGSAYPVNING
                     TTWVEGGGCKAHACGWDFIVTLYNPKTHRVVGYYYNIDPGYLIWFGEIGVHEFAYLVK
                     NYVAAVN"
     gene            725..1135
                     /locus_tag="ACHOT6_004710"
     CDS             725..1135
                     /locus_tag="ACHOT6_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949774.1"
                     /translation="MKTIIKTLLVAVTVLFSVNVAAKTIKLPAGIKYVNSTDEFYCTE
                     IDGLHCQTKNLFKYKGNRYVFVIELGGAWCYDNPMSVLNLETWKAQRLEYNDKRLCSG
                     NNEPFFEIKNGVPTVGIIDTSEKPVIVAQDKLKI"
     gene            1355..2176
                     /locus_tag="ACHOT6_004711"
     CDS             1355..2176
                     /locus_tag="ACHOT6_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFP9949775.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHHVPGIFGEDKHTSRSQN
                     YTYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVQKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTIADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            2176..2421
                     /locus_tag="ACHOT6_004712"
     CDS             2176..2421
                     /locus_tag="ACHOT6_004712"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949776.1"
                     /translation="MKHIPEGVIQVPESPDAPEALPGSRSISDDDLCAWCTRLLYRPG
                     EISLCRLSTGNGIWPSVCDRNGYAYGCPEFQPNIIHP"
     gene            2515..2988
                     /locus_tag="ACHOT6_004713"
     CDS             2515..2988
                     /locus_tag="ACHOT6_004713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFP9949777.1"
                     /translation="MKTWSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGITACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            3004..3480
                     /gene="radC"
                     /locus_tag="ACHOT6_004714"
     CDS             3004..3480
                     /gene="radC"
                     /locus_tag="ACHOT6_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFP9949778.1"
                     /translation="MQQISFLPGEMTTRERSLIQRALKTLDRHLHEPGVGFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGRQVFSFAEHGLL"
BASE COUNT          901 a          906 c          887 g          821 t
ORIGIN      
        1 acctgtcaat tcactcactg gaaatttaat tatgaaatta cctgtaaagt tattaatgag
       61 ccttatgtct ctggtcagcg ttattgcacg tgccggggaa tataaaaatt acagccggga
      121 tgaaataaaa tactggcgat atacatcata caaggggtgg aaacttccgg aaggtttcac
      181 tgatgagata ttttccagtg ccatttacaa cggaagaata tttacaatga aacgtttaca
      241 taccctgatg ttatttctgg cggttctgtt cacgggcttt aactttaacg cagaagcggc
      301 caccgtaaaa caagcactta gttgtaagcc gggagcctgg gctgaacaac ctggagcgtg
      361 tccaacaacg tacgagttat acgaaggtga cgctacctac agagctgcta tcgacaaagc
      421 attaacccct gttgggttaa acggaatgtt tggggaagac ggctatatgg atggtcctga
      481 tggtagtgcg tatcctgtaa acattaacgg aacgacctgg gtagaaggtg gcggttgtaa
      541 ggcccacgcc tgcggctggg actttatcgt aacactctat aacccaaaaa cgcatagagt
      601 cgttggctac tattacaaca tcgaccctgg ttacctgatt tggtttggtg aaattggtgt
      661 ccacgaattc gcgtatctgg tgaaaaacta cgtagctgcg gttaactaat ttaaggagtc
      721 tatcatgaaa acgattatca aaactttact cgttgctgta accgttctgt tctctgttaa
      781 cgtagcagca aaaacaataa agctaccggc tggtatcaaa tacgtcaaca gtaccgatga
      841 attctactgc acagaaatcg acggtttgca ttgccagaca aagaatttgt ttaaatacaa
      901 aggtaacaga tatgttttcg ttattgaact aggtggtgcc tggtgctacg acaaccctat
      961 gtcggtactc aacctggaaa cctggaaagc acagcgcctt gaatacaatg acaagcggct
     1021 atgttcaggt aataacgaac cgttcttcga gatcaaaaat ggtgtcccga ctgtaggtat
     1081 tatcgacacc tctgaaaaac ctgtcattgt agctcaggac aaacttaaaa tctaaggagt
     1141 aaaccatgcg tttctctgca cattattaat atcaaaggta aatacagccc cagccattgt
     1201 taccggaaag ccgcttaatg cgtggctcgc cagtgaaggg cttcccagac aataaacgtc
     1261 attcatttca cccatcaggc cgcgtcttct ccgggagacg tggctttttc atttattaca
     1321 ccagtaaatc ttaaccaccg ataaggagca aagtatgcga ttagccagtc gttttggtta
     1381 tgctgcaaac cagatacgcc gtgaccgtcc gctgacacat gaagagctga tgcaccatgt
     1441 gccggggatt ttcggagaag ataaacacac gtcccgaagc cagaactata cgtacatccc
     1501 caccatcacc gtactggaaa gtctgcagcg ggaaggcttc cagccctttt tcgcctgcca
     1561 gacccgtgtg cgcgacccgg gccgccgggg atacacaaaa cacatgctgc gtctgcggcg
     1621 ggccggagag ataaacggag aacatgtccc tgaaatcatt ctgctcaact cccatgacgg
     1681 tacctccagc taccagatgc tgccgggtta cttccggttc gtctgccaga acgggtgcgt
     1741 ctgtggtcag tctctggggg aagtgcgtgt tccacaccgg ggaaatgtgg tgcagaaagt
     1801 cattgaaggg gcttacgagg tggtgggcgt gtttgaccgg atagaggaga agcgtgatgc
     1861 catgcagtcg ctggtcctgc cgccaccggc acgccaggcg ctggcacagg cggcactgac
     1921 ttaccgttat ggtgacgaac atcagcccgt cactatcgcc gacattctga caccgcgacg
     1981 ccgggaggat tacggtaagg acctgtggag tgcttatcag accatccagg agaatatgct
     2041 gaaaggcggg atttccggtc gcagtgcaaa aggaaaacgt atccacaccc gtgccattca
     2101 cagcatcgac accgatatta agctcaaccg cgcattgtgg gtgatggcag aaacgctgct
     2161 ggagagcatg cgctgatgaa acatatccct gaaggcgtga ttcaggtacc agaatcaccc
     2221 gacgccccgg aagcgctgcc ggggtctcgt tccatttctg atgatgacct gtgcgcctgg
     2281 tgtacccgtc tgctttaccg tcccggagaa atcagtctgt gcagactgag tacaggcaat
     2341 ggcatctggc catccgtctg tgaccggaac ggttacgcat acggctgtcc ggaatttcag
     2401 ccgaatatca ttcatccctg atattaccgg gtctcacggt cagtgatgaa cgtgatgttg
     2461 ccagctcccg acttttactc cctctcattt attcacatta caaggatttc atacatgaaa
     2521 acatggtctc agaacactac ctcttcggcc tgtgcaccgg agactggcct gcaacaactg
     2581 gttgccacca tagtccctga tgaacagcgc atcagtttct ggccgcagca ttttggcctc
     2641 attccacagt gggtgaccct ggagccccgt atcttcggct ggatggaccg tctgtgtgaa
     2701 gactactgcg gtggtatctg gaatctgtat accctgaaca atggcggggc atttatggcc
     2761 cctgaaccgg atgacgatga tgacgaaaca tgggtactgt tcaatgccat gaacggtaac
     2821 cgcgctgaaa tgagcccgga agccgccggc attaccgcct gcctgatgac gtacagccat
     2881 cacgcctgtc gtacagaaaa ttatgccatg acggtccact attaccggct gcgggattac
     2941 gccctgcagc atccggaatg cagcgccatt atgcgcatca ttgactgaaa gggggccgga
     3001 acaatgcaac agatttcctt tctgcccgga gaaatgacaa ccagagagcg cagcctcatt
     3061 cagcgggccc tgaaaaccct ggaccgccat cttcatgaac ccggcgtggg cttcacctcc
     3121 acccgtgcgg cacgggaatg gctgattctg aacatggcgg gactggagcg tgaagagttc
     3181 cgggtactgt atctgaacaa ccagaatcag ctgattgccg gtgaaaccct cttcaccggc
     3241 accatcaacc gcacggaagt ccatccccgg gaagtgatta aacgcgccct gtatcacaat
     3301 gccgctgccg tggtgctggc acacaatcac ccgtccggtg aagtcacacc cagtaaggca
     3361 gaccggctta ttacggagcg tctggtacag gcactggccc tggtggatat ccgggtaccg
     3421 gaccatctga tagttggtgg cagacaggtt ttctcctttg ccgaacatgg tctgctttaa
     3481 cccgtcaccg tcaccaccac atcatacctg ttttc
//
LOCUS       DAZKIV010000267         3495 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZKIV010000267 DAZKIV010000000
VERSION     DAZKIV010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.269"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..909)
                     /gene="cynR"
                     /locus_tag="ACHOT6_004715"
     CDS             complement(10..909)
                     /gene="cynR"
                     /locus_tag="ACHOT6_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414872.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFP9949779.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMANFYARYPGITLQLQEMSQEKIEALLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALDKCAVVGGNESR"
     gene            1019..1678
                     /gene="cynT"
                     /locus_tag="ACHOT6_004716"
     CDS             1019..1678
                     /gene="cynT"
                     /locus_tag="ACHOT6_004716"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFP9949780.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            1709..2179
                     /gene="cynS"
                     /locus_tag="ACHOT6_004717"
     CDS             1709..2179
                     /gene="cynS"
                     /locus_tag="ACHOT6_004717"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFP9949781.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            2185..3366
                     /gene="cynX"
                     /locus_tag="ACHOT6_004718"
     CDS             2185..3366
                     /gene="cynX"
                     /locus_tag="ACHOT6_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFP9949782.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPVRFPQLWVKE
                     A"
BASE COUNT          712 a          947 c          974 g          862 t
ORIGIN      
        1 tgtcagtggt taccgcgatt catttccgcc aacaaccgcg catttatcca acgccatgtg
       61 caaaaatgcc ttcgcggcgg ctgtctgcca gctatttttc cgccgcaaca aaaccgccgt
      121 tctctccagt agtggcgggg caagagaaat cgctttaagc ccgtcatgtt gtgtggcaat
      181 cgctgctggt aacaatgtgg aaagggaagt gcggcgaatc agctccagaa ccgcgctaat
      241 tgagttcgcc tcaatgacca cctgtggatg cagccccgct ttctcgcagt agtggtcaat
      301 ttgctctctg gtggcaaatt ccgcgctgag cagtaccagt ttttcatcat gcaagcgact
      361 caacgccacc tgttcacagg cagccagcgg atgatgttgc gccacgacta acgctaaact
      421 ttctgtcagt aaaggaattg cctccagctc cggcgaatgc acaggcgcga aggcaatccc
      481 aacgtccaac tcgtcgcgac aaagcagagc ttcgattttc tcctgcgaca tttcctgtag
      541 ctggagcgtg atgccggggt agcgcgcata gaaattcgcc attaaagggc cgataaagta
      601 gcttgtaaag gtgggagtga cggcgatacg cagcgatcct cgcgtcagat cggcaacatc
      661 atgaatcgcc cgtttacccg cccccagttc ctgtaacgcc cggctggcgt actgtcgcca
      721 gacttctcct gcatcagtga gacgaatcgt tcgcccgcta cggtcaaaca gcggcacgcc
      781 taaactctcc tctaactggc gaatctgctg ggaaagcgca ggttgggaga cgtgcaacgc
      841 actggctgca cgggtgaagc tgccatgttc agccacggca agaaagtaat tgatatgtcg
      901 agagagcatt cgccaaccta taagtaaatc caatagaact catcataaat gagactttta
      961 ccttatgaca atcggcgagt agtctgcctc tcattccaga gacagacaga ggttaacggt
     1021 gaaagagatt attgatggat tccttaaatt ccagcgcgag gcatttccga agcgggaagc
     1081 cttatttaaa cagctggcga cacagcaaag cccgcgcaca ctttttatct cctgctccga
     1141 cagccgtctg gtccctgagc tggtgacgca acgtgagcct ggcgatctgt tcgttattcg
     1201 caacgcgggc aatatcgtcc cttcctacgg gccggaaccc ggtggcgttt ctgcttcggt
     1261 ggagtatgcc gtcgctgcgc ttcgggtatc tgacattgtg atttgtggtc attccaactg
     1321 tggcgcgatg accgccattg ccagctgtca gtgcatggac catatgcctg ccgtctccca
     1381 ctggctgcgt tatgccgatt cagcccgcgt cgttaatgag gcgcgcccgc attccgattt
     1441 accgtcaaaa gctgcggcga tggtacgtga aaacgtcatt gctcagttgg ctaatttgca
     1501 aactcatcca tcggtgcgcc tggcgctcga agaggggcgg atcgccctgc acggctgggt
     1561 ctacgacatt gaaagcggta gcatcgcagc ttttgacggc gcaacccgcc agtttgtgcc
     1621 actggccgct aatcctcgcg tttgtgccat accgctacgc caaccgaccg cagcgtaacc
     1681 ttatttttaa accatcagga gatccaccat gattcagtca caaattaacc gcaatattcg
     1741 tcttgatctt gccgatgcca ttttgctcag caaagctaaa aaagatctct catttgccga
     1801 gattgccgac ggtaccggtc tggcagaagc cttcgtaacc gcggctttgc tggggcagca
     1861 ggcgcttcct gccgacgccg cccgcctggt cggggcgaag ctggatctcg acgaagacgc
     1921 cattctactg ttgcagatga ttccactgcg tggctgcatt gatgaccgta ttccaaccga
     1981 cccaacgatg tatcgtttct atgaaatgtt gcaggtgtac ggcacaaccc tgaaagcgtt
     2041 ggttcatgag aagtttggcg atggcattat cagcgcgatt aacttcaaac tcgacgttaa
     2101 gaaagtggcg gacccggaag gtggcgaacg tgcggtcatt accttagatg gtaaatatct
     2161 gccgaccaag ccgttctgac agccatgcgc aatcatcaaa agacgttcac gatgctgctg
     2221 gttctggtgc tgattggtct taatatgcga ccactgctca cctccgtcgg tccactgcta
     2281 ccgcaattgc gccaggcgag cggaatgagc tttagcgtgg ctgccctgtt gaccgctctg
     2341 ccggtggtca ccatgggcgg gctggcgctg gccggaagct ggcttcatca gcatgtcagc
     2401 gaacgtcgca gcgtcgccat cagtctgttg ctgattgccg tcggtgcatt gatgcgtgag
     2461 ctttaccccc aaagtgtgct gctgcttagc agcgcactgc ttggtggggt ggggatcggt
     2521 atcattcagg cggtgatgcc ttcagtgatt aaacggcggt ttcagcaacg cacgccactg
     2581 gtgatggggc tgtggtctgc ggctctgatg ggcggcggtg gacttggtgc cgccataacg
     2641 ccctggttag ttcaacatag cgaaacctgg tatcaaacac tcgcctggtg ggcgctgcct
     2701 gccgttgttg cgctctttgc ctggtggtgg caaagcgccc gcgaggtcgc ctcttcccac
     2761 aagacaacaa ccactccggt tcgcgtggta ttcactcccc gcgcgtggac gctgggtgtt
     2821 tacttcggtc tgattaacgg cggttacgcc agcctgattg cctggttacc cgctttctat
     2881 attgagattg gtgccagcgc gcagtacagc ggttccttac tggcattgat gacgcttggg
     2941 caagccgcag gagctttgct gatgcctgct atggctcgcc atcaggatcg gcgcaaactg
     3001 ttaatgctgg cgctggtgtt acaactggtg gggttctgcg gctttatctg gttgccgctg
     3061 caattgccgg tattgtgggc gatggtgtgt gggttaggtc tgggcggcgc gtttccgctc
     3121 tgtttgctgc tggcgctcga tcactctgtg caaccggcta ttgctggcaa gctggtggcg
     3181 tttatgcagg gaatcggttt tatcatcgcc gggcttgccc cgtggttttc tggcgtgctg
     3241 cgtagtatca gcggcaatta cctgatggac tgggcatttc atgcgctgtg cgtcgttggg
     3301 ctgatgatca taaccctgcg ttttgcacca gtacgttttc cgcagctgtg ggtcaaagag
     3361 gcatgatgcg acgcttgttc ctgcgctttg ttcatgccgg atgcggctaa tgtagatcgc
     3421 tgaacttgta ggcctgataa gcgcagcgta tcaggcaatt tttataattt aaactgacga
     3481 ttcaacttta taatc
//
LOCUS       DAZKIV010000268         3485 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.270, whole genome shotgun sequence.
ACCESSION   DAZKIV010000268 DAZKIV010000000
VERSION     DAZKIV010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.270"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="ACHOT6_004719"
     CDS             complement(<1..281)
                     /gene="ybgE"
                     /locus_tag="ACHOT6_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFP9949783.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLI"
     gene            complement(281..394)
                     /gene="cydX"
                     /locus_tag="ACHOT6_004720"
     CDS             complement(281..394)
                     /gene="cydX"
                     /locus_tag="ACHOT6_004720"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFP9949784.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(409..1548)
                     /gene="cydB"
                     /locus_tag="ACHOT6_004721"
     CDS             complement(409..1548)
                     /gene="cydB"
                     /locus_tag="ACHOT6_004721"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFP9949785.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="ACHOT6_004722"
     CDS             complement(1564..3132)
                     /gene="cydA"
                     /locus_tag="ACHOT6_004722"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFP9949786.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
BASE COUNT          983 a          949 c          840 g          713 t
ORIGIN      
        1 aaaatcagcc cgacaatgag aacaatatcg gcaagcaatg ggcaaaaaat cccttgccaa
       61 agaaccttct gcggacgaaa gcccacgccg tgaatcacac cagcacagac ggcccacatc
      121 agcaataaac catgccagat ttccagttca ctggtcttcg cggcaaaacg agatgggtcc
      181 caaaacatac atcctgctaa cagaagcgcc atcacgaagg aaagcgcccg cagggggcgc
      241 ttgtccatta ccgcatacaa agtcgcgata atcttactca tcagatgtct tcttgaccgg
      301 ctttgcctga ttcgacgtgt tcaagcgcca gcgcggtgat taccccaaac gaacaggcaa
      361 gaagcgttcc cagaatccat gcgaaatacc acatttttag ctccttactt agtacagaga
      421 gtgggtgtta cgttcaatat cttctttggt gatacgacca aacattttcc agtaacacca
      481 ggcggtgtag agcagaatga tcggtaccag aaccaccgca acccaggtca tgacgttaag
      541 cgtcagctgg ctggaagttg catcccacat tgtcagactt gcgttcatca tggtgctgga
      601 cggcatcaca aacgggaaca ttgcgatacc ggctgtcagg atgatgcagg ccagcgtcag
      661 ggaggagaac acaaacgccc acgcggcttt atccatacgt gcagtcagga tggtcagcag
      721 cggcagaacc acacccagtg ccggaatagc ccacagaatt ggcgtgttgt tgaagttaac
      781 cagccatgcg ccagcttcac gaaccacttc tttattcagt gggttagagg ctgcgtaatg
      841 gtccattgtc gatttcacga cataaccatc gataccgtac atcacccata cgccagccag
      901 tgcgaaacag accagtgtca ccagcgcagc cacctgagcc gttgcacggg tacgcaggtg
      961 cagttcgccc acggtacgca tttgcagata ggttgcgccc tgagtaatga tcatccctac
     1021 gctcaccacg cctgccagca ggccgaacgg gttaagcaac tggaagaagt taccggtgta
     1081 gtacagacgc agatattcat caacgttgaa cggtacgccc tgcaacaggt taccgaacgc
     1141 tacaccaatt accagcggcg gaacgaagct accaatgaag atgccccagt cccacatgtt
     1201 acgccagcgg gtttcttcaa tcttggagcg gtagtcaaaa ccgaccggac ggaagaacaa
     1261 agacgccagc acgaggatca tcgccacata gaagccggag aacgcagcgg catagaccat
     1321 cggccaggca gcaaagagtg cgccgcccgc ggtgatcagc caaacctggt taccgtccca
     1381 gtgtggtgca atggagttaa tcataattcg acgctcggtg tcgttacgac cgaggaaacg
     1441 ggtgagcatg cccaccccca tgtcgaaacc gtcagtgact gcaaaaccaa tcagcagaac
     1501 gccaaccagc agccaccaga taaaacgcaa tacttcataa tcgatcattt gacgactcct
     1561 gtcttagcgt gccggctgag tagtcgtgga agactgctca aagtgatagc gaccggtttt
     1621 caggctgctt gggccgaggc gtgcaaattt gaacattaag aacaattctg ccaccaggaa
     1681 cagggtatac aggccgcaaa tcagcaccat tgagaagatg agatcgcctg cggtcagtga
     1741 cgagttcgcc acagctgtcg gcagcacttc accgatagcc cacggttggc ggccatattc
     1801 agccacgaac cagcccgctt ctacagcaat ccacggcagc ggaataccgt acagcgcggc
     1861 gcgcagaagc cattttttct cgccaatgcg gttgcggatg acactccaga aagagagcgc
     1921 gatgattgcc agcagcagga agccacacgc caccatgata cggaacgcaa agtacagcgg
     1981 cgctacacgc gggatggagt ctttggttgc ctgttgaatc tgcgcttcag tcgcatcagc
     2041 cacgtttggc gtatagcgtt tcagcagcag accgtaaccg aggtctttct tcatgctatt
     2101 gaactggtca cgaaccgcct ggtcggtaga accagaacgc agttgttcga gcagagagta
     2161 cgccttcatc ccgttacgaa tgcgttcttc atgctgcacc atcagctctt tcaggccgat
     2221 aaccggggta tccacggaac gcgttgcaat gatgcccagt gcgtaaggga tctgaatcgc
     2281 aaatttgttc gtctcctctt cctgatcagg aatgccgaac agagtaaagg cagcaggcgc
     2341 aggttgcgtt tcccactcgg cttcaatagc agccagtttg gttttctgca cgtcgcccat
     2401 ttcgtagccg gattcatcac ccagaacaat aacagacaga acagcagcca taccgaagct
     2461 ggcagcgata gcaaaggagc gtttagcgaa ggcgaagtca cgacctttca gcatatacca
     2521 tgcgctgata ccgaggatga acatcgcgcc agtcacataa ccagacgcta cagtgtgaac
     2581 gaatttcacc tgagcaaccg ggttaagcac cagctcggag aagctcacca tctccatacg
     2641 catagtttca aagttgaaat cggacgcgat tgggttttgc atccagccgt tcgcaaccag
     2701 aatccacagt gcggacaggt ttgaaccgag cgccaccagc caggtgacac acatatgctg
     2761 aactttaccc agacgatccc aaccgaagaa gaacagacct acaaaggtgg attcgaggaa
     2821 gaaggccatc agaccttcga ttgccagcgg cgcaccgaag atatccccta catagtggga
     2881 atagtaagac cagttagtcc cgaactggaa ctccatggtc agaccggtag ccacacccag
     2941 agcgaagttg ataccaaaca acttgcccca gaacttggtc atatctttat aaatctgttt
     3001 gccggagagg acgtagaccg tttccataat ggccagcagg aacgccatac cgagcgtcag
     3061 tggcacaaaa aggaagtggt acatcgcggt caaggcaaac tgtaagcgcg acagttcgac
     3121 tatatctaac atcatgactc cttgctcatc gcatgaagac tccgagagtg aaccccgtta
     3181 gaaagggtca cacgcatgcc ccaatagaaa tttgtttgaa cactttcatc ataccatgtg
     3241 aatgcacaca aaaacggcgt aaagaaaggt tgcaaacatg ttaataaaaa ctcaaattga
     3301 tcccacgtat atattacgcc gcaaaatcct tacaataaac aggtttttgt tgaagcaaat
     3361 ttgcattttt cgacggtgat caatttatag ctaaattacc gcctttcagc caatttgatc
     3421 gagaacaatt tatctctttt tgatgcccat ttccaagact tatacattga taaatatcaa
     3481 ttcct
//
LOCUS       DAZKIV010000269         3482 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZKIV010000269 DAZKIV010000000
VERSION     DAZKIV010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.271"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1345
                     /gene="paoC"
                     /locus_tag="ACHOT6_004723"
     CDS             <1..1345
                     /gene="paoC"
                     /locus_tag="ACHOT6_004723"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFP9949787.1"
                     /translation="LQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAG
                     ANRHTGLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQV
                     DPADPTRRFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLE
                     KSGARVHLEPNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVS
                     AGSGGQWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEAT
                     AGGRLTAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNP
                     KTARSQVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDD
                     TDPISSPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            1355..2311
                     /gene="paoD"
                     /locus_tag="ACHOT6_004724"
     CDS             1355..2311
                     /gene="paoD"
                     /locus_tag="ACHOT6_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFP9949788.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            2362..2700
                     /locus_tag="ACHOT6_004725"
                     /pseudo
     CDS             2362..2700
                     /locus_tag="ACHOT6_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            2986..>3482
                     /locus_tag="ACHOT6_004726"
     CDS             2986..>3482
                     /locus_tag="ACHOT6_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6538 domain-containing protein"
                     /protein_id="HFP9949789.1"
                     /translation="MRYKPNQYIICDSYGNYYLRITLPVYMQPFFEGKRTFVRSLHTS
                     NLRVARRKRDQIADEYHCLRESVAPVNSTIENTLELLRSKAKYAKTATRMQDTASSCP
                     SLLKILEIYLTINSTKKKPATLAKARKAVEMFLSYRKKPDIALQDVSRTTVTGWIEHM
                     QKTLS"
BASE COUNT          817 a          922 c          978 g          765 t
ORIGIN      
        1 ccttcagcac ctgcgtatcg gtgccgacca gagcgggaaa atcacagcta tctcacatga
       61 aagctggtcc ggaaacctgc ccggcggcac gccagaaacg gcggtacagc aaagcgaatt
      121 actctacgcc ggggcgaatc gtcataccgg cctgcggctc gccacgcttg atttgccgga
      181 agggaacgcc atgcgtgcgc ccggcgaagc ccccggtctg atggcgctcg aaatcgcgat
      241 cgacgaactg gcggaaaaag cgggcatcga tcccgtcgag tttcgcatcc tgaatgacac
      301 tcaggttgac cccgccgacc cgacgcgccg cttctctcgc cgtcagctta tcgagtgctt
      361 gcgcaccgga gcggataaat ttggctggaa gcagcgcaac gccacacccg gacaggtgcg
      421 cgacggggag tggctagtcg gccacggtgt tgcggcgggc tttcgcaata atctgctgga
      481 aaaatcgggg gctcgggttc acctcgaacc aaacggcacc gttaccgtgg aaacggacat
      541 gaccgacatt ggcaccggca gctacaccat tctggcccag acggcagcgg aaatgcttgg
      601 cgtaccgctg gagcaggttg cggttcacct cggcgattcc agtttcccgg tttctgcggg
      661 ttctggtgga caatggggcg cgaatacctc cacctccggc gtttacgccg cctgtatgaa
      721 gcttcgcgaa atgattgcct cggcagtcgg gtttgatcct gagcagtcgc agtttgccga
      781 cggcaagatt accaacggta cccgaagcgc cacgctacat gaagccaccg caggcggcag
      841 actgacagcg gaagagagca ttgaattcgg aacactgagc aaagagtacc agcagtcgac
      901 ctttgccggg cattttgtgg aggtcggcgt gcatagcgcg acgggagaag ttcgggtccg
      961 gcgtatgctc gctgtgtgtg ctgcaggacg catcctgaat ccgaaaactg cgcgcagcca
     1021 ggtcattggc gcaatgacta tgggcatggg tgcggcactg atggaggagc tggcggtgga
     1081 tgaccgtttg ggctacttcg ttaatcacga tatggcgggg tatgaggtgc cggtccatgc
     1141 ggatatccca aaacaggagg tgattttcct ggatgatacc gaccccatat cctccccgat
     1201 gaaggccaaa ggtgtcggtg agttgggcct gtgcggcgtg agcgcggcta tcgccaacgc
     1261 ggtgtataac gccaccggta ttcgggtacg ggattatccc atcactctgg ataagctgct
     1321 cgataagctg ccggatgtgg tttaaggaga aacaatgtca tacccgcttt ttgataaaga
     1381 cgaacactgg cataagccag agcaggcgtt tctcaccgat gaccaccgga ccattctgcg
     1441 cttcgccgta gaggcgctaa tgtccggtaa aggagcggtg ctggtgacgc tggtggagat
     1501 acacggcggc gcggcgcgcc cgctcggggc gcagatggtg gttcgcgaag atggtcgtta
     1561 ctgcggtttt gtctctggcg gctgcgtgga ggccgctgcc gcttttgaag cgctggagat
     1621 gatgggctca ggccgcgatc gcgaaattcg ctatggcgaa ggttcgccgt ggtttgacat
     1681 cgttctgccc tgcggcggtg ggatcacgct gacgctccat aaactacgct cggcacagcc
     1741 tctgctcgcc gtgctgaacc gtctggaaca gagaaaaccg gcggggctgc gctacgatcc
     1801 gcaagcacaa tcgttggtgt gcctgcccac acaaacccga acgggctgga atctcaatgg
     1861 ctttgaggtg gggttcaggc catgcgtcag gctgatgatt tacggacgtt ctcttgaggc
     1921 gcaggcaacc gcgagtcttg cagcagccac aggctatgac agccatatct tcgatctttt
     1981 tccggcctca gccagcgctc agatcgatac cgacacggcg gtcattttgc tgtgccatga
     2041 tctcaaccgg gagctgccag cattgcaggc cgcgcgagaa gcaaaaccct tttatctcgg
     2101 cgcattgggc agccatcgaa cccacacgtt acgtctgcaa aagctccacg agctgggatg
     2161 gtccagggag gagacagcgc aaatccgggc ccccgtcggg atattcccca aagcccggga
     2221 tgcgcatact ctggcactct ccgtgctggc agaaatcgcc tctgtacgtc tccatcatga
     2281 ggaggattca tgcctgcccc cgtcgtcctg atccttgcgg ccgggcgtgg agagcgcttt
     2341 ctcgcctccg ggggaaatac ccataagtgt atcggctggc gtcagtcccc ggaggttgcg
     2401 ccttatcgct ggccatttga agaaaacggg agaactttcg accttgcgat tgaaccgcag
     2461 attacgacta atgatctgcg tctgatgttg aggctggctc ttgccggcgg aggaataaca
     2521 attgccactc aggaaacttt caggccatat attgaaagcg gtaagcttgt atcgctgctt
     2581 gatgactttc ttccacaatt tccgggcttc tatctgtatt tcccacagcg tcgcaatatt
     2641 gcaccaaagc tccgcgccct gattgactac gtcaaagaat ggcggcagca attggtttaa
     2701 atagctgcac ctgcactgcc tgatgtcaga acagtatttt gatgaattgc caaggttaca
     2761 atggcacaaa tgcggcacag gaggaaacgt ggtgtattta aatatggggt aactctttga
     2821 ttttaatggc gataatagga gtaaaacact cgattaatct cgtgttaatg ccatcaccta
     2881 cgaccattac taattgattg aatcttatag aaaaaatcat cattttctat gcgttacaat
     2941 caattttcac attacaaacg ctccgacact ttagccgggg agtttatgag atacaagccc
     3001 aatcaataca taatctgtga ttcttacggt aactactatt taaggatcac gctgcctgtg
     3061 tatatgcagc ccttttttga aggaaaaagg acgtttgtca ggagtctaca tacaagtaac
     3121 cttcgtgttg cacgtagaaa gcgtgatcag attgcagatg aataccattg tctacgggag
     3181 agtgtcgctc ctgtaaacag cacaatagag aacacgctgg aactgttacg cagtaaggct
     3241 aaatacgcca aaacagctac cagaatgcaa gatacagcgt cttcgtgtcc gtcattgctt
     3301 aaaattcttg aaatctacct gacaattaac agcacgaaga agaagccagc cactttagct
     3361 aaggcaagaa aagcggtaga gatgtttctc tcctaccgta aaaagcctga tattgcattg
     3421 caagatgtaa gccgcaccac tgttacaggc tggattgaac acatgcaaaa aaccctttca
     3481 ca
//
LOCUS       DAZKIV010000270         3385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZKIV010000270 DAZKIV010000000
VERSION     DAZKIV010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.272"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..149
                     /locus_tag="ACHOT6_004727"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACHOT6_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            931..1908
                     /locus_tag="ACHOT6_004728"
     CDS             931..1908
                     /locus_tag="ACHOT6_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFP9949790.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSNQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(2192..2932)
                     /locus_tag="ACHOT6_004729"
     CDS             complement(2192..2932)
                     /locus_tag="ACHOT6_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9949791.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(3056..3175)
                     /locus_tag="ACHOT6_004730"
                     /pseudo
     CDS             complement(3056..3175)
                     /locus_tag="ACHOT6_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            3247..3385
                     /locus_tag="ACHOT6_004731"
                     /pseudo
     CDS             3247..3385
                     /locus_tag="ACHOT6_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144897.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT          879 a          819 c          846 g          841 t
ORIGIN      
        1 aaggtgttca gtataaaagc aataaataca gaaagttact ttatggggta atgcaaagta
       61 tgagtcgcag agggaattgc ctggataatt cgccgatgga aagagtcttt cgcagcctta
      121 aaagtgaatg gcttccgaaa ggtggttatg gtgattgcca tgcagtacgc gaataaatca
      181 gtggataaat gcttattaca acgtttatcg acctcataca aacaatggtg gtttatcgcc
      241 ttgccgtatg aagaaaaatg gaaacaggtt attccggtgt cctgatttta tgatccacta
      301 cacattcctg acgaacgcca ctgcataaat cacattgcac atcctcagag agtacgcagt
      361 ataggagggg tatatataca ttatcggtat atcaggcgac ttctgtctgt atgttgtcgg
      421 tgctcgccgc agaccggtga ccgggcgaag cccgcgaacc ggcgaggaag cgcaatgtat
      481 ctgtagttac agtgagcagg tacgccctga agccctttca taaaacggtt gtgtgcgctg
      541 tccttcggga agatttatcc acatatccag aggcccgatc cagtaaacag atccatgaat
      601 gatcaataaa ggatccatta aagatcccca taccgctgca aaccttgcca ctcatgctcc
      661 agaaccacga tcacataagc tgtggcatgt tgatgataaa ctgtaacatg ctgatgataa
      721 gctgtattca gtaatacata tactgacgta agttaatgac ataaactatg gtcagtacac
      781 cagactcagt tgttaaatac agactgcagg tttttcttca gtcagttgac gggggtctga
      841 cacacgattt tctgtttatt cttttactgt ccacaggcag gaggctttct ggaaaacgaa
      901 aattcagaca tcaaaaaact gttcggcgag gtggataagt cctccggtga gctggtgaca
      961 ctgacaccaa acaataacaa caccgtacaa cctgtggcgc tgatgcgtct gggcgtcttt
     1021 gtaccgaccc ttaaatcact gaagaacagt aaaaaaaata cactgtcacg tactgatgcc
     1081 acggaagaac tgacacgtct ttccctggcc cgtgcagagg gattcgataa ggttgagatc
     1141 accggccccc ggctggatat ggataacgat ttcaagacct gggtggggat cattcattcc
     1201 tttgcccgcc ataacgtgac tggtgacaaa gttgaactac cttttgtcga gtttgcaaaa
     1261 ctgtgtggta taccttcaaa ccagtcatca cgcaggctgc gtgagcgcat cagcccttcc
     1321 ctgaaacgca ttgccggtac cgtgatctcc ttttcccgca ccgatgagaa gcacacccgg
     1381 gaatacatca ctcatctggt acagtcagcc tactacgata ctgaacggga tattgttcag
     1441 ttacaggctg atccccgcct ttttgaactg taccagtttg acagaaaagt tcttctccag
     1501 cttaaggcga ttaatgccct gaagcgacgt gagtccgccc aggcactcta cacctttata
     1561 gagagcctgc cccgggatcc ggcaccgata tcgctggcgc ggctgcgtgc acgcctcaat
     1621 ctgaagtctc cggtattttc ccagaaccag acggtcagac gggcaatgga gcaattgcgt
     1681 gagattggat atcttgatta cactgagatc cagcggggac ggacaaaact cttctgtatt
     1741 cactaccggc gtccccggtt aaaagcgccg aatgatgaga gtaaggaaaa tccgttgcca
     1801 ccttcacctg cggaaaaagt cagtccggag atggcggaga agcttgccct gcttgagaaa
     1861 ctgggcatca cgctggatga cttggaaaaa ctcttcaaat cccgctgaac ataaactgta
     1921 gtcagtgaag atcgctcctt tactgactac agcttatatt aaccggggca gtgagtggtt
     1981 tgctcactgc agtttatatt ctgtttcctg cagtgctgtc tgtagctgag ctgccatctg
     2041 ctgtccctta cgtaagtgat cccgtaacct gatgctgagg cattgctccc ttcataaaac
     2101 atgacttact cactacagct tatatacatg ctccagctta tgttatgtct gttctgctga
     2161 ccactgctta tataaggaag cgcgtatgcc gtcacctcag gggaggcagt gtacgcagga
     2221 tctccgcagc atcccgcccg tcacctgtga aaggcactgc cagcgtggca gccatatcca
     2281 gtgcaaacac tctggtataa acctccatag aacgtggatc cctgtgacca gccagtgcct
     2341 gaatgacttt ccggggctgg cggtgataaa gcatgtgcat gatatagctg tgccggaagg
     2401 tgtgtggtgt aaccggaatc gaaaagtgta ccccgtcagc ttcggcccgt ctgacagcct
     2461 gcttcagcca gttgcgcatg gtttcgtcgg tcacggccca taatggttca cgacgacggg
     2521 gccgggtggt gatcatccag ctttccatct gcctgacata gcttatatct gtcagcggaa
     2581 ctaggcggac ttcatctttt ggcgggcgtc cgcgtcgcgc acgcactttt tcggacagga
     2641 tccgcacaaa cggtcttgca ccatccagat caaatgattc cggcgtcagc atccgggctt
     2701 cgccaatacg cattccggta ttccagaggg tggcgaacag catatggtga cgctgatccg
     2761 gcatatagaa gagaagggca ctcacttccg gggccagcag gtaggccggt gtggcccccg
     2821 tggaagtggc cattcttctc agggagagcg ctgttgcaaa atccaccccc ggcgcaatgg
     2881 gtaacaggga gacgcgttct ggtgaattct gcaggggaat gacattgttc atgagttatt
     2941 tttcacgtag cacacgcact gtttatggat acaggtattc tgacctgaac gcccggatcc
     3001 ttccacgaca ataaatcgca gcgcgcggat ccttttgttt gattatcacg cattgttgcg
     3061 ggtaaaacca aatacgtaaa ggcgcatttt gccgcaggta tcccgtctta agccagtcat
     3121 ggtgcgggtt gagagctggt ttaccgtttt tgggagaatt ggggtaatgc caggagaggc
     3181 agacatgggt aaacagagtt cagacaggaa agaagcgcag gtacgttacg taaatcctaa
     3241 ccaagttttg atatgcgatt cgaacgcact gcattatctg ctgtagaaaa tagcttccgg
     3301 atattcagta gttctctggg aatatcccga tggaaaaata tgcagcagat aaatgaatat
     3361 ggcaatagag tgcaatttat tcatt
//
LOCUS       DAZKIV010000271         3383 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZKIV010000271 DAZKIV010000000
VERSION     DAZKIV010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.273"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            52..1203
                     /gene="yiaY"
                     /locus_tag="ACHOT6_004732"
     CDS             52..1203
                     /gene="yiaY"
                     /locus_tag="ACHOT6_004732"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFP9949792.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1368..2906
                     /gene="aldB"
                     /locus_tag="ACHOT6_004733"
     CDS             1368..2906
                     /gene="aldB"
                     /locus_tag="ACHOT6_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFP9949793.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3125..3328)
                     /locus_tag="ACHOT6_004734"
     CDS             complement(3125..3328)
                     /locus_tag="ACHOT6_004734"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949794.1"
                     /translation="MAFQQVEVYHIEIMELLTKLMNNSSKTSTVQIKRIKPSIIYRLL
                     LIGLGTPMVIYGLVCPLTIETRD"
BASE COUNT          838 a          832 c          907 g          806 t
ORIGIN      
        1 gaaaatcctc agtaagctgc ccaccctttt ttacactttc aggagtgtgt tatggcatct
       61 tcaactttct ttattccttc tgtgaatgtc atcggcgctg attcattgac tgatgcaatg
      121 aatatgatgg cagattatgg atttacccgt accttaattg tcactgacag tatgttaacg
      181 aaattaggta tggcgggcga tgtgcaaaaa gcactggaag aacgcaatat ttttagcgtt
      241 atttatgatg gcacccaacc taaccccacc acggaaaacg tcgccgcagg tttgaaatta
      301 cttaaagaga ataattgcga tagcgtgatc tccttaggcg gtgggtctcc acacgactgc
      361 gcaaaaggta ttgcgctggt ggcagccaat ggcggcgata ttcgcgatta cgaaggcgtt
      421 gaccgctctg caaaaccgca gctgccgatg atcgccatca ataccacggc gggtacggcc
      481 tctgaaatga cccgtttctg catcatcact gacgaagcgc gtcatatcaa aatggcgatt
      541 gttgataaac atgtcactcc gctgctttct gtcaatgact cctctctgat gattggtatg
      601 ccgaagtcac tgaccgccgc aacgggtatg gatgccttaa cgcacgctat cgaagcgtat
      661 gtttctattg ccgccacgcc gatcactgac gcttgtgcac tgaaagccgt gaccatgatt
      721 gccgaaaacc tgccgttagc cgttgaagat ggcagtaatg cgaaagcgcg tgaagcaatg
      781 gcttatgccc agttcctcgc cggtatggcg ttcaataatg cttctctggg ttatgttcat
      841 gcgatggcgc accagctggg cggtttctac aacctgccac acggtgtatg taacgccgtt
      901 ttgctgccgc atgttcaggt attcaacagc aaagtcgccg ccgcacgtct gcgtgactgt
      961 gccgctgcaa tgggcgtgaa cgtgacaggt aaaaacgatg cggaaggtgc tgaagcctgc
     1021 attaacgcca tccgtgaact ggcgaagaaa gtggatatcc cggcaggcct acgcgacctg
     1081 aacgtgaaag aagaagattt cgcggttctg gcgactaatg ccctgaaaga tgcctgtggt
     1141 tttactaacc cgatccaggc aactcacgaa gaaattgtgg cgatttatcg cgcagcgatg
     1201 taatcatcat ttccacaacg gctggcaaat tgtcagccgc tttttcaact atctctgtaa
     1261 cccttgcccg taaattcgtg atagctgtcg taaagctgtt accgactggc gaagatttcg
     1321 ccagtcacgt ctacccttgt tatacctcac accgcaagga gacgatcatg accaataatc
     1381 ccccttcagc acagattaag cccggcgagt atggtttccc cctcaagtta aaagcccgct
     1441 atgacaactt tattggcggc gaatgggtag cccctgccga cggcgagtat taccagaatc
     1501 tgacgccggt gaccgggcag ctgctgtgcg aagtggcgtc ttcgggcaaa cgagacatcg
     1561 atctggcgct ggatgctgcg cacaaagtga aagataaatg ggcgcacacc tcggtgcagg
     1621 atcgcgcggc gattctgttt aagattgccg atcgaatgga acaaaacctc gagctgttag
     1681 cgacagctga aacctgggat aacggcaaac ccattcgcga aaccagtgct gcggatgtac
     1741 cgctggcgat tgaccatttc cgctatttcg cctcgtgtat tcgggcgcag gaaggtggga
     1801 tcagtgaagt tgatagcgaa accgtggcct atcatttcca tgaaccgtta ggcgtggtgg
     1861 ggcagattat cccgtggaac ttcccgctgc tgatggcgag ctggaaaatg gctcccgcgc
     1921 tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct taccccgctt tctgtactgc
     1981 tgctaatgga aattgtcggt gatttactgc cgccgggcgt ggtgaacgtg gtcaatggcg
     2041 caggtgggga aattggcgaa tatctggcga cctcgaaacg catcgccaaa gtggcgttta
     2101 ccggctcaac ggaagtgggc caacaaatta tgcaatacgc aacgcaaaac attattccgg
     2161 tgacgctgga gttgggcggt aagtcgccaa atatcttctt tgctgatgtg atggatgaag
     2221 aagatgcctt tttcgataaa gcgctggaag gctttgcact gtttgccttt aaccagggcg
     2281 aagtttgcac ctgtccgagt cgtgctttag tgcaggaatc tatctacgaa cgctttatgg
     2341 aacgcgccat ccgccgtgtc gaaagcattc gcagcggtaa cccgctcgac agcgtgacgc
     2401 aaatgggcgc gcaggtttct cacgggcaac tggaaaccat cctcaactac attgatatcg
     2461 gtaaaaaaga gggcgctgac gtgctcacag gcgggcggcg caagctgctg gaaggtgaac
     2521 tgaaagacgg ctactacctc gaaccgacga ttctgtttgg tcagaacaat atgcgggtgt
     2581 tccaggagga gatttttggc ccggtgctgg cggtgaccac cttcaaaacg atggaagaag
     2641 cgctggagct ggcgaacgat acgcaatatg gcctgggcgc gggcgtctgg agccgcaacg
     2701 gtaatctggc ctataagatg gggcgcggca tacaggctgg gcgcgtgtgg accaactgct
     2761 atcacgctta cccggcacat gctgcgtttg gtggctacaa acaatcaggt atcggtcgcg
     2821 aaacccacaa gatgatgctg gagcattacc agcaaaccaa gtgcctgctg gtgagctact
     2881 cggataaacc gttggggctg ttctgatata agaagctggt cgcattgggt attcattgcc
     2941 tgatgcgacg cttacgcgtc ttatcatgcc tacgggaacc tgaccgtagg ccggataagg
     3001 cgtacacgcc gcatccggca taaacaaagc gcacgttgtt acaatttacg ttttaaacct
     3061 acgtgcgcaa taacccagac tacgccaaca gaagtgcgtt tctggggcgt gttttgccag
     3121 gagtttaatc tcgcgtttcg atggtgagcg ggcaaaccag gccgtaaatc accattggcg
     3181 ttccgaggcc aatcagcaat aaacggtaga taattgaagg tttaatacgc tttatctgta
     3241 ctgtggatgt tttactggag ttattcatta gcttggtcaa tagttccatt atttcaatat
     3301 gataaacctc gacctgttga aaagccacgc agcacacttc agaaagtata taaaaaacaa
     3361 tatgccgtta tataaataag gaa
//
LOCUS       DAZKIV010000272         3346 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZKIV010000272 DAZKIV010000000
VERSION     DAZKIV010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.274"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..304
                     /locus_tag="ACHOT6_004735"
     CDS             <1..304
                     /locus_tag="ACHOT6_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFP9949795.1"
                     /translation="KDLNVMYHNAKVATNNLKLGTFGAFDNEWHTLAFRFAGNNSLQV
                     TPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRNATYPVLIDSIAVEVNNADAAA"
     gene            441..620
                     /locus_tag="ACHOT6_004736"
     CDS             441..620
                     /locus_tag="ACHOT6_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HFP9949796.1"
                     /translation="MTFIHQLMLYFCTVVCVLYLLSGGYRAMRDFWRRQIDKRAAEKI
                     SASQSAGSKPEEPLI"
     gene            661..906
                     /locus_tag="ACHOT6_004737"
     CDS             661..906
                     /locus_tag="ACHOT6_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9949797.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDA
                     ILDELLGAPAVPEPEGIAGEGSASDGGEPTPDSDMMM"
     gene            983..1198
                     /locus_tag="ACHOT6_004738"
     CDS             983..1198
                     /locus_tag="ACHOT6_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9949798.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIKEDRRKAARGE"
     gene            1203..1736
                     /locus_tag="ACHOT6_004739"
     CDS             1203..1736
                     /locus_tag="ACHOT6_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9949799.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALSCWGIDR"
     gene            2011..2580
                     /locus_tag="ACHOT6_004740"
     CDS             2011..2580
                     /locus_tag="ACHOT6_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9949800.1"
                     /translation="MNMMTVPFHGDSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQRFASTITEIVMVAEDGKRRNMVSLPLRKLAGWLQTINPNKVKPEIRGKVIQYQEEC
                     DDVLYEYWTKGFVVNPRRMSVMEELNQACADMKRDKNIASVFATGLNEWKQVKSAHVS
                     KIRTLINEANLLIDFVLADTGKGKITKAD"
     gene            2580..2729
                     /locus_tag="ACHOT6_004741"
     CDS             2580..2729
                     /locus_tag="ACHOT6_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949801.1"
                     /translation="MEWWLMISDKLITLAKILCVIVGISFLVMLVAIFFSTAWRVLTL
                     SGLVG"
     gene            2737..3204
                     /locus_tag="ACHOT6_004742"
     CDS             2737..3204
                     /locus_tag="ACHOT6_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFP9949802.1"
                     /translation="MNRVLCVVIIVLLVACGALSLGLNHYRDNAITYKEQRDKKVSEL
                     ELANATITDMQQRQRDVAALDARYSRELADARAENETLRADVAAGYRSLRINATCPGP
                     VREATGTARVDNATGPRLADTAERDYFTLRERLMTMQKQLEGAQDYIRTQCLN"
BASE COUNT          867 a          758 c          948 g          773 t
ORIGIN      
        1 aaaagacctg aatgtgatgt accacaatgc gaaagtggcg acaaacaacc tgaaactggg
       61 aacctttggc gcatttgata acgaatggca tacgctggct ttccgctttg ccgggaataa
      121 cagccttcag gtgacgccgg ttattgatgg tcaggatggc acaccgttca cgctgacgca
      181 gtcaccggtc agtgcatttg cggcggataa actgcatgtg acagacatta ccaggaatgc
      241 gacttacccg gtgctgattg acagcattgc ggtggaagtg aacaacgcgg atgccgcggc
      301 atgataaaaa aagccgccag ttgcctgaga cctctggcgg tgtgagattc atggagaatc
      361 aaggaaagat actaccactt tcgtcatcgg catttttaaa tgaaaactgt ttacttagtc
      421 aaccataacg gtaagaaatt atgacattta ttcatcagct gatgctgtac ttctgtacgg
      481 tggtctgtgt gctgtatctc ctttcgggtg ggtacagggc catgcgtgac ttctggcgca
      541 gacagattga caaaagggcc gctgagaaaa tcagcgccag tcagtcagcc ggaagcaaac
      601 ccgaagagcc gctcatttag cggcaacttt cttaatcaca tctttcgacg agaaaatccc
      661 atgtcagaaa ttacatccct ggtcactgct gaggcagtga aggaagtcct gcgctctgaa
      721 gaagtccgga gtgcactgaa acagaaactc cgccagaatc ttgagtcgcg tcttgatgca
      781 gaagtggatg ctattctgga tgaactgctc ggcgcaccgg cagttcctga gccggaaggt
      841 atcgcgggtg aggggagtgc ttcagatggc ggtgaaccca cacctgacag cgacatgatg
      901 atgtaagcat gcgcaagggg ccatcggtgt gtgccggtgg tctttatatt gttgtgagct
      961 tccggattgc gggagacggg gtatgtacca gatggaaaaa atcacaacag gtgtgtcata
     1021 caccacgtca gcggtgggaa cgggctactg gttcctgcag ttgctggaca gggtttcccc
     1081 gtctcagtgg gcggcaatag gcgtgctggg gagtctgctg tttgggctgc tgacatatct
     1141 gacgaacctg tatttcaaga ttaaagaaga ccggcgtaag gcggcacggg gagagtaagc
     1201 tgatgagcag gaaactccgc tatggtttat cggctgccgt tctggcgctg attgccgcag
     1261 gtgcttctgc gcctgaaatc ctcgaccagt ttctggatga aaaggaaggt aaccacacca
     1321 cggcataccg tgatggtgcg ggtatctgga ccatctgccg tggagccacc cgggtggatg
     1381 gtaagcctgt gattcctggc atgaagctgt cgaaggaaaa atgcgaccgg gttaacgcta
     1441 tcgaacggga taaggcgctg gcatgggtgg agcgtaatat aaaagttcca ctgaccgagc
     1501 cacaaaaagc aggtatcgcg tcattctgtc cgtacaacat tggtcccggt aagtgtttcc
     1561 cgtcgacgtt ttacagacga attaatgcag gtgatcgaaa aggtgcctgc gaagcgattc
     1621 gctggtggat taaggacggt ggcagagact gccgtatccg ctcaaataat tgctacggtc
     1681 aggtatcaag acgtgaccag gagagtgcgc tgtcatgctg gggtatcgac aggtaagcag
     1741 aatattttgc tgaaaaatga cgttggccaa cgcgggcaga taacacgaaa tcctgcgaac
     1801 tggcaaaatg taagtgaata aagtcaacaa gattgtttca tgaagaggca ccgtaatggt
     1861 gcctttgtca tttctgcgct tcgcacaagc gtaaataaac caaagaacct ttcaggatga
     1921 gccctggtgg ataaccggca gtggtctggt taaccctctt tgggctggtt attcctgtgc
     1981 gcagggttca tcactaaaag gaaataaccg atgaatatga tgaccgtgcc gtttcacggc
     2041 gattctcttt atgtggttaa ccataacggc gaaccatacg ttcccatgaa acctgtcgtt
     2101 gcggggatgg ggctggcctg gcaatcacag ttggctaagt taagacagcg ttttgcgtca
     2161 actataacgg aaatcgttat ggttgctgag gatgggaaac gacgcaatat ggtgtccctg
     2221 ccgcttcgaa aacttgcagg ctggttacaa accatcaatc ccaacaaagt aaaacccgaa
     2281 atccgcggca aggtaatcca gtatcaggaa gagtgtgacg atgttctcta tgaatactgg
     2341 acgaagggtt ttgtcgttaa tccccgtcga atgagtgtga tggaagaact caatcaggct
     2401 tgcgctgaca tgaaacggga taaaaacatt gccagtgtgt ttgctaccgg gctgaatgag
     2461 tggaaacagg ttaaatccgc gcatgtatca aaaatccgca cattgataaa cgaagcgaat
     2521 ctgctgattg attttgtcct ggctgataca ggcaaaggga aaataacaaa ggcggattga
     2581 tggagtggtg gctaatgata tcggataaac tcataacgct ggcgaagatc ctctgtgtaa
     2641 tcgtcggcat ttcattttta gtcatgctgg ttgccatttt cttttccacc gcctggcgag
     2701 tcctgacgtt atcgggactg gtggggtgaa agagagatga accgtgttct gtgtgtggtg
     2761 attattgtcc tgctggtagc ctgtggtgcg cttagtctgg ggctgaatca ttaccgcgat
     2821 aacgccataa cctacaaaga gcagcgcgat aaaaaagtca gtgagctgga gctggcaaat
     2881 gcaaccatta ctgatatgca gcagcgccag cgtgatgttg ctgcacttga tgccagatac
     2941 tcgagggaat tagccgatgc gagagctgaa aatgaaactc ttcgcgctga cgttgccgct
     3001 ggttaccgca gcctgcggat caacgccacc tgtccaggtc ccgtgcgtga agccaccggc
     3061 accgcccgcg tggataatgc aaccggcccc cgactggcag acaccgctga acgggattat
     3121 ttcaccctca gagaacggtt gatgacaatg cagaagcagc tggaaggggc acaggactat
     3181 atccgcactc agtgcctgaa ctaagttttg ctgatgcgcc gtatcgtcac cgtattcctg
     3241 cattaacaga gaccgcagcc cgacagggag actcctctgc gagagtgtgc ggggataatc
     3301 aaaaacgata cacaccgggg tttaccgcgt taacggagcg cggtgt
//
LOCUS       DAZKIV010000273         3342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZKIV010000273 DAZKIV010000000
VERSION     DAZKIV010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.275"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            339..1655
                     /gene="shiA"
                     /locus_tag="ACHOT6_004743"
     CDS             339..1655
                     /gene="shiA"
                     /locus_tag="ACHOT6_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFP9949803.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPENKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDDQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHNQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            1757..3211
                     /gene="amn"
                     /locus_tag="ACHOT6_004744"
     CDS             1757..3211
                     /gene="amn"
                     /locus_tag="ACHOT6_004744"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFP9949804.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          764 a          822 c          810 g          946 t
ORIGIN      
        1 tctcaacaat gatgctaaaa acatacctaa cctcgcctcc ctactggtta taatgcaatg
       61 cagtctatca gactcatcag ggtgccattt tgtgcatatg cggactttta tgtttcatat
      121 ctcgaacctg tggggcctct gcttaatcct taaacaacac cagcaactcc tgcgctttca
      181 tcttccagcg aatttttcat gttgccgcta atcagccata aaatcatttg cagatgcgct
      241 ctgtcgaggt agtctcataa ggttcgttta tagatcgacg gcaatgtgag ttaccttttc
      301 catactaatt ataaaaagac agtacaaaca ggatcattat ggactccacg ctcatctcca
      361 ctcgtcccga tgaagggacg ctttcgttaa gtcgcgcccg acgagctgcg ttaggcagtt
      421 tcgctggtgc cgtcgtcgac tggtatgatt ttttactcta tggcatcacc gccgcactgg
      481 tgtttaatcg cgagtttttc ccgcaagtaa gcccggctat gggaacgctc gccgcatttg
      541 ccacctttgg cgtcggattc cttttccgtc cgctcggcgg tgtcattttc ggtcactttg
      601 gcgaccgact gggacgtaag cgcatgttaa tgctgaccgt ctggatgatg ggcatcgcga
      661 cagccttgat tggtattctt ccttcattct cgaccattgg gtggtgggca cctattttgc
      721 tggtgacact gcgtgccatt cagggatttg ccgtcggcgg cgaatgggga ggcgcggcgt
      781 tgctttccgt tgaaagtgcg ccggaaaata aaaaagcctt ttacagtagc ggtgtacaag
      841 ttggctacgg agtaggttta ctgctttcaa ccggactggt ttcattgatc agtatgatga
      901 caactgacga tcagttttta agctggggct ggcgcattcc tttcctattt agcatcgtac
      961 tggtactggg agcattgtgg gtgcgcaatg gcatggaaga gtccgcggaa tttgaacaac
     1021 agcaacataa tcaagcggcc gcgaaaaaac gcatcccggt tatcgaagcg ctgttacgac
     1081 atcccggtgc tttcctgaag attattgcgc tacgactgtg cgaattgctg acgatgtaca
     1141 tcgttactgc ctttgcactt aattattcaa cccagaatat ggggttaccg cgcgaacttt
     1201 tccttaatat tggtttgctg gtaggtggat taagctgcct gacaattccc tgttttgcct
     1261 ggcttgccga tcgttttggt cgccgtaggg tttatatcac aggtgcgtta atcggaacgt
     1321 tgagcgcatt tcctttcttt atggcgcttg aagcacaatc tattttctgg atagttttct
     1381 tctccataat gctggcaaac attgcgcatg acatggtggt gtgtgtgcaa caaccgatgt
     1441 ttaccgaaat gtttggtgcc agttatcgct atagtggcgc tggagtcggt tatcaggttg
     1501 ccagtgtggt tggcggtgga tttacacctt ttattgccgc tgcactcatc acttactttg
     1561 ccgggaactg gcatagcgtc gccatttatt tgctggctgg atgcctgatt tccgcaatga
     1621 ccgctttgtt gatgaaagac agtcaacgcg cttgatagcc tggcgaagat catccgatct
     1681 tcgccttaca cttttgtttc acatttctgt gacatactat cggatgtgcg gtaattgtat
     1741 ggaacaggag acacacatga ataataaggg ctccggtctg accccagctc aggcactgga
     1801 taaactcgac gcgctgtatg agcaatctgt agtcgcatta cgcaacgcca ttggcaacta
     1861 tattacaagt ggcgaattac ctgatgaaaa cgcccgcaaa caaggtcttt ttgtctatcc
     1921 atcactgacc gtaacctggg acggtagcac aaccaatccc cccaaaacgc gcgcatttgg
     1981 tcgctttacc cacgcaggca gctacaccac cacgattact cgccctactc tctttcgttc
     2041 gtatcttaat gaacaactta cgttgctgta tcaggattat ggtgcgcata tctcagtgca
     2101 accctcgcag catgaaatcc cttatcctta tgtcatcgat ggctctgaat tgacacttga
     2161 tcgctcaatg agcgctgggt taactcgcta cttcccgaca acagaactgg cgcaaattgg
     2221 cgatgaaact gcagacggca tttatcatcc aactgaattc tccccgctat cgcattttga
     2281 tgcgcgccgc gtcgattttt cccttgcacg gttgcgccat tataccggta cgccagttga
     2341 acattttcag ccgttcgtct tgtttaccaa ctacacacgt tatgtggatg aattcgttcg
     2401 ttggggatgc agccagatcc tcgatcctga tagtccctac attgcccttt cttgtgctgg
     2461 cgggaactgg atcaccgccg aaaccgaagc gccagaagaa gccatttccg accttgcatg
     2521 gaaaaaacat cagatgccag catggcattt aattaccgcc gatggtcagg gtattactct
     2581 ggtgaatatt ggcgtgggac cgtcaaatgc taaaaccatc tgcgatcatc tggcagtgct
     2641 acgcccggat gtctggttga tgattggtca ctgtggcgga ttacgtgaaa gtcaggccat
     2701 tggcgattat gtacttgcac acgcttattt acgcgatgac cacgttcttg atgcggttct
     2761 gccgcccgat attcctattc cgagcattgc tgaagtgcaa cgtgcgcttt atgacgccac
     2821 caagctggtg agcggcaggc ccggtgagga agtcaaacag cggctacgta ctggtactgt
     2881 ggtaaccaca gatgacagga actgggaatt acgttactca gcttctgcac ttcgttttaa
     2941 cttaagccgg gccgtagcaa ttgatatgga aagtgcaacc attgccgcgc aaggatatcg
     3001 tttccgcgtg ccatacggga cactactgtg tgtttcagat aaaccgttgc atggcgagat
     3061 taaacttccc ggccaggcta accgttttta tgaaggcgct atttccgaac atctgcaaat
     3121 tggcattcgg gcgatcgatt tgctgcgcgc agaaggcgac cgactgcatt cgcgtaaatt
     3181 acgaaccttt aatgagccgc cgttccgata ataagttctg aaccttctta tcagaaatag
     3241 atgtgaagga gtaagtaaga cacctggcaa atagcctgca ataatcgtgg gctgtttgct
     3301 tccttgggcg gatacgtgtt ttattatcgt cttaatgatt tc
//
LOCUS       DAZKIV010000274         3340 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZKIV010000274 DAZKIV010000000
VERSION     DAZKIV010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.276"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..305
                     /locus_tag="ACHOT6_004745"
     CDS             <1..305
                     /locus_tag="ACHOT6_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001439589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949805.1"
                     /translation="NAEPEEQVDPRKAAVEAAIARAKARKREQQQANAEPEEQVDPRK
                     AAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
     gene            306..1364
                     /gene="rsxD"
                     /locus_tag="ACHOT6_004746"
     CDS             306..1364
                     /gene="rsxD"
                     /locus_tag="ACHOT6_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFP9949806.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            1368..1988
                     /gene="rsxG"
                     /locus_tag="ACHOT6_004747"
     CDS             1368..1988
                     /gene="rsxG"
                     /locus_tag="ACHOT6_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFP9949807.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            1992..2687
                     /gene="rsxE"
                     /locus_tag="ACHOT6_004748"
     CDS             1992..2687
                     /gene="rsxE"
                     /locus_tag="ACHOT6_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFP9949808.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            2687..3322
                     /gene="nth"
                     /locus_tag="ACHOT6_004749"
     CDS             2687..3322
                     /gene="nth"
                     /locus_tag="ACHOT6_004749"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFP9949809.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQYNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT          789 a          858 c          900 g          793 t
ORIGIN      
        1 ctaatgcgga accagaagaa caggttgatc cgcgcaaagc cgccgtcgaa gccgctattg
       61 cccgagccaa agcgcgcaaa cgggaacagc aacaggctaa tgcggaacca gaagaacagg
      121 ttgatccgcg caaagccgcc gtcgaagccg ctattgcccg tgccaaagcg cgcaagctgg
      181 aacagcaaca gactaacgca gaaccagaag aacaggttga tccgcgcaaa gcggcagttg
      241 ccgcggctat tgcccgcgct caggccaaaa aagccgccca gcagaaggtt gtaaacgagg
      301 actaaatggt attcagaata gctagctccc cttataccca taaccagcgc cagacatcgc
      361 gcattatgct gttggtgttg ctcgcagccg tgccaggaat cgcagcgcaa ctgtggtttt
      421 ttggttgggg tactctcgtt cagatcctgt tggcatcggt tagtgctctg ttagccgaag
      481 ctctcgtact caaactacgc aagcagtcgg tagccgcaac gttgaaagat aactcagcat
      541 tgctgacagg cttattgctg gcggtaagta ttccccccct cgcgccatgg tggatggtcg
      601 tgctgggtac ggtgtttgcg gtgatcatcg ctaaacagtt gtatggcggt ctggggcaaa
      661 acccgtttaa tccggcaatg attggttatg tggtcttact gatctccttc cctgtgcaga
      721 tgaccagctg gttaccgcca catgaaattg cggtcaacat ccctggtttt atcgacgcca
      781 tacaggttat tttcagcgga cataccgcca gtggtggtga tatgaacaca ctacgcttag
      841 gtattgatgg cattagtcag gcgacaccgc tggatacatt taaaacctct gtccgtgccg
      901 gtcattcggt tgaacagatt atgcaatatc cgatctacag cggtattctg gcgggcgctg
      961 gttggcaatg ggtaaatctc gcctggctgg ctggcggcct gtggttgcta tggcagaaag
     1021 cgattcgctg gcatattccc ctcagcttct tagtaacgct ggcgttatgc gcaacgttgg
     1081 gctggttgtt ctcaccagaa acactggcag caccgcaaat tcatctgctg tctggtgcga
     1141 ccatgctcgg cgcattcttt attttgactg acccggttac cgcttctacg accaatcgtg
     1201 gtcgtcttat tttcggcgcg ctggcgggct tattagtctg gttgatccgc agtttcggcg
     1261 gctatcctga cggcgtggct tttgccgtcc tgctggcgaa catcacggtt cctctgatcg
     1321 attactacac gcgtccgcgc gtctacggcc atcgcaaagg gtaaaccatg ctgaaaacta
     1381 tccgaaaaca cggcattacg ttggcgctat ttgcagcggg ttcaacaggg ttaactgcgg
     1441 ccatcaacca gatgaccaaa acgacgattg ctgaacaggc cagtctgcaa caaaaggcgt
     1501 tatttgatca ggtgctgcca gccgaacgct ataacaatgc gctggcacag agttgctatc
     1561 tggtaactgc gccagagtta ggtaaaggtg agcatcgggt ttacatcgcc aaacaggatg
     1621 acaaaccggt agccgccgtt ctggaagcaa ccgcgccaga tggctattcc ggtgcgattc
     1681 agctgctggt gggagccgat tttaacggca cggtacttgg cacgcgcgtg acagagcacc
     1741 acgaaacgcc agggcttggc gataaaatcg aactgcgcct ttctgactgg atcacccatt
     1801 ttgcgggtaa aaaaatcagt ggtgcagatg atgcgcactg ggcggtgaag aaagatggtg
     1861 gtgatttcga ccagttcacc ggcgcgacga ttactccccg cgcggtggtt aatgcggtaa
     1921 aacgcgccgg attgtacgct cagacgttac cggcacaact ttctcaactt cctgcctgtg
     1981 gagaataaag cgtgagcgaa attaaagacg ttattgttca ggggttgtgg aaaaacaact
     2041 ctgcgctggt ccagctgctc ggcctttgtc ctctgttggc ggtcacgtcc actgccacta
     2101 acgctctggg tttaggactt gcgactacgc tggtactgac gctgaccaac ctgaccattt
     2161 cgacgctgcg tcactggacg ccagccgaga tccgcattcc catttacgtg atgatcatcg
     2221 cctcggtggt cagcgctgta cagatgctga tcaacgccta cgcctttggc ctgtatcaat
     2281 cattagggat ttttattccg ctgattgtca ctaactgtat cgttgtgggc cgcgctgaag
     2341 ccttcgccgc caaaaaaggt ccggcgcttt cggcactgga cggcttttca attggtatgg
     2401 gcgcaacctg cgccatgttc gtgctgggtt cactacgcga aattatcggc aatggcacat
     2461 tgtttgacgg tgcagatgcg ctgttaggta gctgggcaaa agtattacgc gtggaaattt
     2521 tccacaccga ctcccctttc ctgctggcga tgctgcctcc aggtgcattt attggcctgg
     2581 gactgatgct ggcaggaaaa tacctgattg atgaaagaat gaaaaagcgc cgtactgaag
     2641 cagctgccga acgtgcattg ccaaacggtg aaacagggaa tgtctgatga acaaagcaaa
     2701 acgcctggag atcctcactc gcctgcgtga gaacaatcct catcccacca ccgagcttaa
     2761 tttcagttcg ccttttgaat tgctgattgc cgtactgctt tccgctcagg cgaccgatgt
     2821 cagtgttaat aaggcgacgg cgaaacttta cccggtggcg aatacgcctg cagcgatgct
     2881 tgaactgggc gttgaagggg ttaaaaccta tatcaaaacg attgggcttt ataacagcaa
     2941 agcagaaaat atcatcaaaa cctgccgtat cttactggag cagtataatg gcgaggttcc
     3001 ggaagatcgt gctgcgcttg aagccctgcc cggcgtaggt cgtaaaacag ccaacgtcgt
     3061 attaaacact gcattcggct ggccgactat tgctgtcgac acgcacattt tccgcgtttg
     3121 taatcgtact caatttgcgc cggggaaaaa cgtcgaacag gtagaagaaa agctactgaa
     3181 agtggttccg gcagagttta aagtcgactg ccaccattgg ttgatcctgc acgggcgtta
     3241 tacctgcatt gcccgcaagc cccgctgtgg ctcttgtatt attgaagatc tttgtgaata
     3301 caaagagaaa gttgacatct gaagaaaagg ggtaacaccg
//
LOCUS       DAZKIV010000275         3286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZKIV010000275 DAZKIV010000000
VERSION     DAZKIV010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.277"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..706)
                     /locus_tag="ACHOT6_004750"
     CDS             complement(<1..706)
                     /locus_tag="ACHOT6_004750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFP9949810.1"
                     /translation="MIIDAHTHIGDFVKISMPEDVFLVSLDKYNIDFALCSCGSAVEV
                     DHDQNPIPDEDQVTQHDNNERMLRLVRQHAKRIGAFMWIKPRLESCDQDFEDMIASNR
                     DIIYGIKVHPYHSKMAFNSEKVQDYIRLAQKYDLPVVSHTSNDYESSPQLVYEMAQKY
                     PDVNFVMCHMGLATDNQEAIELIAKLPNLYGDTAWVRADMVYQAIKICGSDKILFGTD
                     NPINGLDTYNDDDFYNK"
     gene            complement(728..2527)
                     /locus_tag="ACHOT6_004751"
     CDS             complement(728..2527)
                     /locus_tag="ACHOT6_004751"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HFP9949811.1"
                     /translation="MITVSSLKHSAKSEDSYAYDNETLHVRLRTLRGEVDKVILWIGD
                     PYNWAEGGLDGGNMAGTDAFGWIGGNEIEMEQEAVTEFHDHWFAVFKPQKRRCRYGFI
                     LFGKEGEKFLFGEKRCVDISSPECEERELSRLNNFFCFPYLNKIDVLNTPSWVKNTVW
                     YQIFPDRFCNGRPEISPEVVEPWGSTPTSFNFMGGDLWGVIDKLDYLEDLGIDGIYFC
                     PIFTSASNHKYDTIDHFSVDPHLGGNIAFHTLIEEAHKRGIKIMLDAVFNHLGADSPI
                     WLDVVRNGANSRYADWFWIHKFPVYPDTPKSEWDFKNFNYETFGNVIEMPKLNTENEE
                     CREYLLSIVRYWTQNFDIDGWRLDVANEVDHHFWRDFRKVIKDIQPECYILGEIWHEG
                     TPWLRGDQFDSLMNYPLTYGIIDYFALQDTTKQEFMTSVTRSYLCYPKNITEVMFNLL
                     DSHDTARILSVCLNDKRKVKLAYLFMLTQAGSPCIYYGSEIGIDGFKSMTLENNRKCM
                     IWDENKQDLELRQFIRWLIRLRKKHPQWCEASIQWKDVEHPTVIAYQRDNITFFLNNS
                     EDTANFIYDGRSMEISGFSYEIEGLPAADLYDF"
     gene            complement(2545..>3286)
                     /locus_tag="ACHOT6_004752"
     CDS             complement(2545..>3286)
                     /locus_tag="ACHOT6_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HFP9949812.1"
                     /translation="IMASFKKGNNLFSSTFSGIEFTFDHYITLFTDTPYMQWYLNTFI
                     LATANMLISLFVVTMTAYVFSRYRFRGKRNTLMSILVLQMFPAFLSMTAIYILLSKAN
                     LIDTYTGLLLVYVTGSLPFMTWLVKGYFDAIPTSLDEAAKIDGAGHLTIFIQIILPLA
                     KPILVFVGLVSFTGPWMDFILPTLVLRSEDKMTLAIGIFSWIASNSAENYTLFAAGAL
                     LVAVPITLLFVFTQKHITTGLVSGAVKE"
BASE COUNT         1076 a          711 c          548 g          951 t
ORIGIN      
        1 acttattata aaaatcatca tcattgtagg tatctagccc attaatagga ttatcggtac
       61 cgaataaaat cttatcacta ccacagattt ttatcgcctg gtaaaccata tccgccctaa
      121 cccacgcagt atcgccatag agattaggaa gttttgcaat caattcgatt gcttcctgat
      181 tatcagtcgc gagccccata tgacacataa caaaattcac gtctggatac ttttgtgcca
      241 tctcataaac taactgtggg ctggattcat aatcattaga agtatggcta actaccggga
      301 gatcatattt ttgagcaagg cgtatataat cctgaacttt ttctgagttg aaagccattt
      361 ttgaatgata agggtgaact ttgatgccat aaatgatatc tcgattgctg gcaatcatat
      421 cttcaaagtc ttgatcacaa ctttccagac gaggcttaat ccacataaac gcgccaattc
      481 gctttgcatg ttgacgaaca agtctgagca tacgttcatt gttatcatgc tgtgtaactt
      541 gatcttcatc cggaatcggg ttctggtcat gatcaacttc aacggcagat ccacaggaac
      601 ataacgcaaa atcaatatta tacttgtcca gagataccag aaaaacatct tctggcatgc
      661 tgattttcac aaaatcacca atatgagtgt gtgcatcaat tatcataaca aacatcctaa
      721 ttgaaattta aaaatcataa agatcagcag caggtaaacc ttcaatttca taactgaaac
      781 cggagatctc catacttctt ccgtcataaa taaagttggc agtatcttca ctattattta
      841 agaagaaagt gatgttatcg cgttgataag cgatcaccgt aggatgttca acatctttcc
      901 attgtattga tgcctcacac cattgtggat gttttttacg tagacgtatt aaccaacgta
      961 taaattgccg taattcaaga tcttgtttgt tttcatccca tatcatacat tttctattat
     1021 tctcaagcgt cattgattta aaaccatcaa ttcctatttc gctaccgtag tagatacaag
     1081 gagagcccgc ttgagttaac ataaataaat aggcgagttt aacttttcgt ttatcattca
     1141 aacaaacgct taaaatacgc gcggtgtcat gactatccag aagattaaac ataacttcag
     1201 ttatattttt aggataacaa agataagaac gtgtaacgga tgtcataaat tcttgttttg
     1261 ttgtatcctg taacgcaaaa taatcaataa tcccatatgt taagggataa ttcattaatg
     1321 aatcaaactg atcaccacgc aaccaggggg tgccctcatg ccatatctca cccaaaatat
     1381 aacattcagg ttggatatct ttaatcacct ttctgaaatc tcgccagaaa tgatgatcca
     1441 cttcattcgc aacatccaga cgccaaccat caatatcaaa attttgcgtc caatagcgaa
     1501 ctatactaag taagtattca cggcactctt cattctcagt atttagcttg ggcatttcaa
     1561 taacattgcc aaaggtttca tagttaaagt ttttaaaatc ccattcactt tttggagtat
     1621 cgggataaac aggaaattta tgaatccaga accaatcggc gtaacgtgaa ttggcaccat
     1681 tgcgtacaac gtcaagccat attggagaat cagctcctaa atgattaaat actgcatcca
     1741 acataatttt tattccacgc ttatgcgctt cttcaatcaa agtatggaaa gcaatattcc
     1801 cgcctaaatg tggatcaaca ctaaaatgat cgatggtgtc atatttatgg ttggaagctg
     1861 aggtaaaaat aggacaaaaa taaatcccat caatacccag atcttccaga taatcgagtt
     1921 tatcaataac cccccacaga tccccaccca taaaattgaa tgaagtcggt gtactgcccc
     1981 acggttctac cacttctggg gatatttcag gtctgccatt acagaaacgg tctggaaaaa
     2041 tttggtacca taccgtattt tttacccagg agggcgtatt tagaacgtca attttattaa
     2101 gataagggaa gcaaaaaaag ttatttaagc ggcttaactc tcgttcttcg cactccggtg
     2161 aactgatatc aacacacctt ttctcaccaa aaagaaactt ctctccctct ttcccaaaaa
     2221 gtataaaacc gtaacggcaa cggcgttttt gaggtttgaa cactgcaaac cagtgatcat
     2281 gaaattcggt gacagcttct tgttccatct cgatctcgtt gccgccaatc cagccgaacg
     2341 cgtctgtccc ggccatgtta ccgccgtcaa gaccaccttc agcccaatta tatggatcgc
     2401 ctatccagag aataacttta tcaacttctc ctcttaatgt gcgtaaccgt acatgcaaag
     2461 tttcgttatc ataggcatag ctatcttcac tttttgctga gtgttttaac gaactaactg
     2521 taatcataaa tactaatccc taaattattc cttaacggca ccgctaacaa gtccagtagt
     2581 tatatgcttc tgtgtgaata caaataacaa tgtgataggt actgcaacta aaagcgcccc
     2641 agcagcaaaa agcgtataat tctctgctga attagaagca atccaactaa aaatgccaat
     2701 cgccagggtc attttatcct cactacgaag caccaacgtt ggcaaaatga agtccatcca
     2761 tggtccagtg aacgaaacta atccaacgaa aacgagaatc ggcttagcca atggcaatat
     2821 tatctgaata aaaatagtca gatgccctgc tccatctatt ttagcggcct catccaaaga
     2881 agttggaata gcatcaaaat aaccttttac caaccacgtc ataaagggca atgaaccggt
     2941 tacataaacc agaagcaacc cagtatatgt gtctatcaaa ttggcctttg atagcaggat
     3001 ataaattgca gtcattgata aaaatgcagg aaacatttgc aatacaagaa tactcattaa
     3061 cgtattacgc tttccacgaa atcgatacct tgaaaaaacg taggcggtca tcgtcacaac
     3121 gaacagtgaa attaacatgt tagcggtagc aagaatgaaa gtgttcaaat accactgcat
     3181 atatggcgta tccgtgaaca acgttatgta gtggtcaaat gtaaactcga tgccactaaa
     3241 tgttgaacta aatagattat tccctttttt aaaagatgcc attatc
//
LOCUS       DAZKIV010000276         3179 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZKIV010000276 DAZKIV010000000
VERSION     DAZKIV010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.278"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..1814
                     /gene="ydeN"
                     /locus_tag="ACHOT6_004753"
     CDS             132..1814
                     /gene="ydeN"
                     /locus_tag="ACHOT6_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9949813.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1866..3023
                     /gene="ydeM"
                     /locus_tag="ACHOT6_004754"
     CDS             1866..3023
                     /gene="ydeM"
                     /locus_tag="ACHOT6_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFP9949814.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKFELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2989..3111)
                     /locus_tag="ACHOT6_004755"
     CDS             complement(2989..3111)
                     /locus_tag="ACHOT6_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949815.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          964 a          685 c          633 g          897 t
ORIGIN      
        1 ctttatggac tgttatttcc ctttttttag ctaaatctgc tatctcttaa tgtgacttac
       61 ttcacttaca tccacttatt tctcttcgta aaattacttc ggaattaagt acaataagaa
      121 gaggaacatt tatgaagtct gcattaaaga aaagtgtcgt aagtacctcg atatctttga
      181 tactggcatc tggtatggct gcatttgctg ctcatgcggc agatgatgta aagctgaaag
      241 caaccaaaac aaacgttgct ttctcagact ttacgccgac tgaatacagt accaaaggaa
      301 agccaaatat tatcgtacta accatggatg atcttggtta tggacaactt ccttttgata
      361 agggatcttt tgacccaaaa acaatggaaa atcgtgaagt tgtcgatacc tacaaaatag
      421 ggatagataa agccattgaa gctgcacaaa aatcaacgcc gacgctcctt tcattaatgg
      481 atgaaggcgt acgttttact aacggctatg tggcacacgg tgtttccggc ccctcccgtg
      541 ccgcaataat gaccggtcga gctcccgccc gctttggtgt ctattccaat accgatgctc
      601 aggatggtat tccgctaaca gaaactttcc tgcctgaatt attccagaat catggttatt
      661 acactgcagc agtaggtaaa tggcacttgt caaaaatcag taatgtgccg gtaccggaag
      721 ataaacaaac gcgtgactat catgacaact tcaccacatt ttctgcggaa gaatggcaac
      781 ctcaaaaccg tggctttgat tactttatgg gattccacgc tgcaggaacg gcatattaca
      841 actccccttc actgttcaaa aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc
      901 agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa aacacttgac cagcctttta
      961 tgctttacct ggcttataat gctccgcacc tgccaaatga taatcctgca ccggatcaat
     1021 atcagaaaca atttaatacc ggtagtcaaa cagcagataa ctactacgct tccgtttatt
     1081 ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa gaaaaacgga cagtatgaca
     1141 atacaattat tctctttacc tccgataatg gtgcggttat cgatggtcct ctgccgctga
     1201 acggggcgca aaaaggctat aagagtcaga cctatcctgg cggtactcac accccaatgt
     1261 ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg
     1321 atttctaccc gacagcactt gatgcagccg atatcagcat tccaaaagac cttaagctgg
     1381 atggtgtttc cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc
     1441 tgacctggat aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata
     1501 attaccacaa atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg
     1561 acttaagcca attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag
     1621 aaaacaatca gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg
     1681 ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca
     1741 gccagccacc gcttagcgaa gtaaatcagg agaagtttaa caatatcaag aaagcactaa
     1801 gcgaagcgaa ataactaaac cttcatgcgg cggaattttc cgccgcctta ttgagcgaga
     1861 tagagatgca cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact
     1921 gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc
     1981 tgaaagagtt catcaaacaa tatatcgcag cgtctggcaa tcaggtctat tttacctggc
     2041 aaggcggtga acccactctg gctggcctgg attttttccg taaagttatt cactatcaac
     2101 aacgctatgc aggccaaaaa cgtattttta atgcattaca aacgaatggc attttattga
     2161 ataatgaatg gtgtgccttt ctcaaagaac atgaatttct ggttggtatc tcgatcgatg
     2221 gcccccagga gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa
     2281 aagtgatagc agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg
     2341 tcattaataa cgttaatgtc cattaccctc ttgaggttta tcatttttta aaatctatcg
     2401 gcagtaaaca tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca
     2461 gtggccatag tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg
     2521 gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca
     2581 ttcgtcagtt tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt
     2641 tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg
     2701 accattttgt ctatccacag tacaaaattg gaaacattaa taaatttgaa ctcaaaacga
     2761 tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg
     2821 catataaacc tatctgcaat ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg
     2881 agactgtttc ttatttttgc gaaggttata aaatcctttt ttcaaccatg gtaccttata
     2941 tgaacgccat ggttgagtta gctaagaaca gagtaccgct ttaccacatt atggatgttg
     3001 caaaacaaat ggagaataat taataacgct ctcttttaac tccattctta gcctccttct
     3061 tatgacctta acaaggcacc cgacataaat tcctgtcggg tgtttcgtca tcatcatctt
     3121 cccttgcgaa aatcctggct tacgtggtat gcgtcagggc attagcaata cccttgatt
//
LOCUS       DAZKIV010000277         3133 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZKIV010000277 DAZKIV010000000
VERSION     DAZKIV010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.279"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ACHOT6_004756"
     CDS             complement(265..1296)
                     /gene="rsmC"
                     /locus_tag="ACHOT6_004756"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFP9949816.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TRLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1399..1812
                     /gene="holD"
                     /locus_tag="ACHOT6_004757"
     CDS             1399..1812
                     /gene="holD"
                     /locus_tag="ACHOT6_004757"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFP9949817.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1781..2227
                     /gene="rimI"
                     /locus_tag="ACHOT6_004758"
     CDS             1781..2227
                     /gene="rimI"
                     /locus_tag="ACHOT6_004758"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFP9949818.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2242..2919
                     /gene="yjjG"
                     /locus_tag="ACHOT6_004759"
     CDS             2242..2919
                     /gene="yjjG"
                     /locus_tag="ACHOT6_004759"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFP9949819.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
BASE COUNT          743 a          843 c          826 g          721 t
ORIGIN      
        1 ccatcaattc ttaaaaagaa ttgctaccac ggaggcgcat tctagtggtt ttcagctttt
       61 cgtcaatagt taattatcgc cggaggtgta attgctggaa aaatgtccat caggaaacta
      121 gcgtgcaggt ttggtaggca tgcgggggca gatgccagat gcgacgctgg cgcgtcttat
      181 ctggcctacg aagggctaac gtgcaggttt tgtaggtcgg ataaggcgtt cacgccgcat
      241 ccgacacggt attcggcgag ataattaacc tttcttcgcc tggcgggtca taatggcgcg
      301 atacaccttg aaacgcccgg tttgcgcaat cacttcatgg aagccaaatg tctcatccag
      361 cacgtccggg taaggcagga aggcgttcgc tacaattcgc agctcgccgc cgctattaag
      421 atgacgcacc gcgccgcgaa tcagcgtttg cgccgcatcc agtctggttt gcatcccatc
      481 gtggaacggc gggttggaga tgatcatatc aaaacgacct ttcacctcgg aaaagacgtt
      541 gctggcaaag acttcacctt caacacagtt ggccgcaagt gttgcgcggc tggcttctac
      601 cgctggcgca gagacatcgc acaaggtgag acgaattttc ggcgaatggc gcgcaaaggc
      661 aactgaaagc acccccgcgc cacagccgac atccagcact ttacctttcg tgtgcggagt
      721 taacgtcgag agcagcaact ggctaccgac atccagaccg tcgcggctaa acacgccagg
      781 cagcgttttg accgtcaggc catcgacgct gtattcgccc cagaatttat cggcatcaaa
      841 taccggctgt ttttccagac gaccaaaata gaggccacag cgacgagcgc tgtcgacttt
      901 attcaacggc gcataatctg ccagcatctg ctcggcgctg cgcacgccgc tgcggttctc
      961 gccaacgaca aaaatatccg tccccaccgg cagcagagaa agtaaattca tcaactggaa
     1021 ctgggcttcc ggtttgttct tcggccagta gtaaatcagc gtatcgcaat ctgcgacgtc
     1081 atccgccgtg gcgaccagac tgaaacgggc gttatccccc atctggcggc ttaatacctg
     1141 ccagtggtgg aattgctggg tatgagcacg gctggccgcg gtatctaaac gcgcgggcag
     1201 gtcatcctgt aagtctccgg caaacagaat acggctttgt tcgaaatcat cactgtgacg
     1261 cagcaagact tcacttgccg gggtaaatgc agacatggaa tgctcctcaa ttgatactgg
     1321 cggcgattat agccatatgt tggcgcggta tcgacgaatt tgctatattt gcgcccctga
     1381 caacaggagc gattcgctat gacatcccga cgagactggc agttacagca actgggcatt
     1441 acccagtggt cgctgcgtcg ccctggcgcg ttgcaggggg agattgccat tgcgatcccg
     1501 gcacacgtcc gtctggtgat ggtggcaaac gatcttcccg ccctgaccga tcctttagtg
     1561 agcgatgttc tgcgcgcatt aaccgtcagc cccgatcaag tgctgcaact gacgccagaa
     1621 aaaatcgcga tgctgccgca aggcagtcgc tgcaacagct ggcggttggg tactgacgaa
     1681 ccgctatcac tggaaggcgc tcaggtggca tcaccggcgc tcaccgaatt acgggcaaac
     1741 ccaacggcac gcgccgcgtt atggcaacaa atttgcacat atgaacacga tttcttccct
     1801 cgaaacgact gatttaccag cggcttacca cattgaacaa cgcgcccacg cctttccgtg
     1861 gagtgaaaaa acctttgcca gcaaccaggg cgagcgttat ctcaactttc agttaacgca
     1921 aaacggcaaa atggcggcgt ttgcgattac gcaagtggtg ctggatgaag ctacattgtt
     1981 caatattgcg gtcgatcctg actatcagcg tcagggattg ggaagggtgc tgctggaaca
     2041 tctgatcgac gaactggaaa aacgcggagt ggcgacacta tggctggaag tccgtgcctc
     2101 aaatgctgcc gccattgccc tgtacgaaag tttaggcttt aacgaggcga cgattcgccg
     2161 caattactac cccaccacgg acggtcgcga agacgccatc atcatggcgt tgccaatcag
     2221 tatgtaagac aaggtggaat aatgaagtgg gactggattt tctttgatgc cgatgaaacg
     2281 ctgtttacct ttgactcatt caccggcctg cagcggatgt ttcttgatta cagcgtcacc
     2341 tttaccgctg aagattttca ggactatcag gccgttaaca agccactgtg ggtggattat
     2401 caaaacggcg cgatcacttc attacagctt cagcacgggc gtttcgagag ctgggccgaa
     2461 cggctgaaag ttgaagcagg cttgcttaac gatgccttta ttaatgcgat ggcggaaatc
     2521 tgtacgccgt tgccgggcgc ggtttctctg cttaacgcca ttcgtggcaa cgctaaaatc
     2581 ggcatcatca ccaacggttt tagcgccttg cagcaagtgc gtctggaacg cacgggcctg
     2641 cgtgattact tcgatttgct ggtgatttcc gaagaagttg gcgttgccaa accgaataag
     2701 aaaattttcg attatgcgct ggaacaggcg ggcaatcctg accgttcacg cgtgctgatg
     2761 gttggcgaca ctgccgagtc cgatattctc ggtggcatca acgccgggct tgcgacctgc
     2821 tggctgaatg cgcaccatcg cgagcaacca gaaggcatcg cgcccacctg gaccgtttct
     2881 tcgttgcacg aactggagca gctcctgtgt aaacactgat tgcctccccc ccgttgatgg
     2941 gtaaaatagc cgcaattttt cgttttcaac aagcgcggcg cgatgccgct tactcaagaa
     3001 gaaagaatta tgacgttgtc tccttatttg caagaggtgg cgaagcgccg cacttttgcc
     3061 attatttctc accccaataa ttaagcttaa attaaagctc ttttactttt tcaaaaccct
     3121 ttcacttaat tga
//
LOCUS       DAZKIV010000278         3108 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZKIV010000278 DAZKIV010000000
VERSION     DAZKIV010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.280"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2814
                     /gene="evgS"
                     /locus_tag="ACHOT6_004760"
     CDS             <1..2814
                     /gene="evgS"
                     /locus_tag="ACHOT6_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFP9949820.1"
                     /translation="DALTNEVRYEVSQNWLDTGNLAFLNKPLELTEHEKQWIKQHPNL
                     KVLENPYSPPYSMTDENGSVRGVMGDILNIITLQTGLNFSPITVSHNIHAGTQLSPGG
                     WDIIPGAIYSEDRENNVLFAEAFITTPYVFVMQKAPDSEQTLKKGMKVAIPYYYELHS
                     QLKEMYPEVEWIQVDNASAAFHKVKEGELDALVATQLNSRYMIDHYYPNELYHFLIPG
                     VPNASLSFAFPRGEPELKDIINKALNAIPPSEVLRLTEKWIKMPNVTIDTWDLYSEQF
                     YIVTTLSVLLVGSSLLWGFYLLRSVRRRKVIQGDLENQISFRKALSDSLPNPTYVVNW
                     QGNVISHNSAFEHYFTADYYKNAMLPLENSDSPFKDVFSNAHEVTAETKENRTIYTQV
                     FEIDNGIEKRCINHWHTLCNLPASDNAVYICGWQDITETRDLINALEVEKNKAIKATV
                     AKSQFLATMSHEIRTPISSIMGFLELLSGSGLSKEQRVEAISLAYATGQSLLGLIGEI
                     LDVDKIESGNYQLQPQWVDIPTLVQNTCHSFGAIAASKSIALSCSSTFPDHYLVKIDP
                     QAFKQVLSNLLSNALKFTTEGAVKITTSLVHIDDNHAVIKMTIMDSGSGLSQEEQQQL
                     FKRYSQTSAGRQQTGSGLGLMICKELIKNMQGDLSLESHPGIGTTFTITIPVEISQQV
                     ATVEAKAEHPITLPEKLSILIADDHPTNRLLLKRQLNLLGYDVDEATDGVQALHKVSM
                     QHYDLLITDVNMPNMDGFELTRKLREQNSSLPIWGLTANAQANEREKGLSCGMNLCLF
                     KPLTLDVLKTHLSQLHQVAHIAPQYRHLDIEALKNNTANDLQLMQEILMTFQHETHKD
                     LPAAFQALEAGDNRTFHQCIHRIHGAANILNLQKLINISHQLEITPVSDDSKPEILQL
                     LNSVKEHIAELDQEIAVFCQKND"
     gene            complement(2870..>3108)
                     /locus_tag="ACHOT6_004761"
     CDS             complement(2870..>3108)
                     /locus_tag="ACHOT6_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFP9949821.1"
                     /translation="EVWLARIHEVGVPVAPLLSVAEAIKLPQTQARNMLIEAGGIMMP
                     GNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
BASE COUNT          958 a          673 c          635 g          842 t
ORIGIN      
        1 gatgctttaa caaatgaagt tcgctatgaa gtatcacaaa attggcttga tacaggaaac
       61 ctggcctttc tgaacaaacc attagaactc actgaacatg aaaaacagtg gattaagcag
      121 catcccaatt taaaggtgct ggaaaatcct tactcgcccc cctattctat gacggatgaa
      181 aatggctcgg ttcggggcgt tatgggggac attcttaata ttattacctt gcaaacaggt
      241 ttaaattttt ctccgatcac cgtttcacac aatatccatg ctggaacaca gcttagcccc
      301 ggaggatggg atataatacc tggcgctatt tatagtgaag atcgagaaaa taatgtttta
      361 tttgctgaag ccttcataac aacgccttac gtttttgtca tgcaaaaagc gcctgacagt
      421 gaacaaacat taaaaaaagg aatgaaagtt gccattccat attattatga gctgcattcg
      481 caattaaaag agatgtatcc ggaggttgaa tggatacagg tcgataatgc cagcgctgca
      541 tttcacaagg ttaaggaagg tgaacttgat gctctggtcg cgacacagtt aaattcgcgt
      601 tacatgatcg atcattacta tcctaatgaa ctttatcatt ttcttattcc tggcgttccg
      661 aatgcatcgc tttcgttcgc ttttcctcgc ggagaaccgg aacttaagga tattattaat
      721 aaagcactga atgcaattcc cccaagcgaa gttctgcgcc tgacggaaaa atggattaaa
      781 atgcccaatg tgaccattga cacatgggac ctatatagcg agcaatttta tattgttacg
      841 acattatccg ttttattagt tggcagtagc cttttatggg gattctacct gttacgctca
      901 gttcgtcgtc gtaaagtcat tcagggtgat ttagaaaacc aaatatcatt ccgaaaagca
      961 ctctcggatt ccttaccgaa tccaacttat gttgtaaact ggcaaggtaa tgtcattagt
     1021 cataatagtg cttttgaaca ttatttcact gcggattact acaaaaatgc aatgttacca
     1081 ttagaaaaca gtgactcacc ctttaaagat gttttttcta atgcgcatga agtcacagca
     1141 gaaacgaaag aaaatcgaac aatatacaca caggtatttg aaattgataa tggcatcgag
     1201 aaaagatgca ttaatcactg gcatacatta tgcaatcttc ctgcaagtga caatgcagta
     1261 tatatttgtg gttggcaaga tattactgaa acgcgtgatc taattaatgc actcgaggta
     1321 gaaaaaaata aagcgataaa ggctaccgta gcaaaaagtc agtttctggc aacgatgagt
     1381 cacgaaataa gaacaccaat aagctctatt atgggcttcc tggaacttct gtcgggttct
     1441 ggtcttagca aggagcaacg ggtggaggcg atttcacttg cctacgccac cggacaatca
     1501 ctcctcggct taattggtga aatccttgat gtcgacaaaa ttgaatcggg taactatcaa
     1561 cttcaaccac aatgggtcga tatccctact ttagtccaga acacttgtca ctctttcggt
     1621 gcgattgctg caagcaaatc gatcgcatta agttgcagca gtacatttcc tgatcattac
     1681 ctggttaaga tcgaccctca ggcgtttaag caggtcttat caaatttact gagcaatgct
     1741 ctcaaattta ccaccgaggg ggcagtaaaa attacgacct ccctggttca cattgatgac
     1801 aaccacgctg ttatcaaaat gacgattatg gattctggaa gtggattatc gcaggaagaa
     1861 caacaacaac tctttaaacg ctacagccaa acaagtgcag gtcgccagca aacaggatct
     1921 ggcttaggct taatgatctg caaagaatta attaaaaata tgcagggcga tttgtcatta
     1981 gaaagtcatc caggcatagg aacaacattt acgatcacaa tcccggtaga aattagccag
     2041 caagtggcga ctgtcgaggc aaaagcagaa caccccatca cactacctga aaagttgagc
     2101 atattaatcg cggatgatca tccgaccaac aggctattac tcaaacgcca gctaaatcta
     2161 ttaggatatg atgttgatga agccactgat ggtgtgcaag cgctacacaa agtcagtatg
     2221 caacattatg atctgcttat tactgacgtt aatatgccga atatggatgg ttttgagttg
     2281 actcgcaaac ttcgtgagca aaattcttcc ttacccatct gggggcttac agccaacgca
     2341 caggctaacg aacgtgaaaa agggttaagt tgcggcatga acttatgttt gttcaaaccg
     2401 ttgaccctgg atgtactgaa aacacattta agtcagttac accaggttgc gcatattgca
     2461 cctcagtatc gccaccttga tatcgaagcc ctgaaaaata atacggcgaa cgatctacaa
     2521 ctgatgcagg agattctcat gactttccag catgaaacgc ataaagatct acccgctgcg
     2581 tttcaagcac tagaagctgg cgataacaga actttccatc agtgtattca tcgcatccac
     2641 ggtgcggcta acatcctgaa tttgcaaaag ttgattaata ttagccatca gttagaaata
     2701 acacctgttt cagatgacag taagcctgaa attcttcagt tgctgaactc tgtaaaagaa
     2761 cacattgcag agctggacca ggagattgct gttttctgtc agaaaaatga ctaaatagcg
     2821 gctcccacaa tgttcaaatg tgggagctat ttaccagcac atcttacatt tatgatgaga
     2881 actcctggcg aatttgttcc ccatgctggt cgagcgttgc cgctcccggc ataacatgcg
     2941 ggtccgcgca gccgctgatt tttatcggat tacccggcat cattattccc ccggcttcaa
     3001 tcaacatatt tctcgcctga gtttgtggca atttaatggc ctcagccaca cttaataacg
     3061 gcgcgacggg tacaccaact tcatgtattc tggctaacca aacttcag
//
LOCUS       DAZKIV010000279         3096 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZKIV010000279 DAZKIV010000000
VERSION     DAZKIV010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.281"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            48..377
                     /locus_tag="ACHOT6_004762"
     CDS             48..377
                     /locus_tag="ACHOT6_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949822.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            377..1075
                     /locus_tag="ACHOT6_004763"
     CDS             377..1075
                     /locus_tag="ACHOT6_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9949823.1"
                     /translation="MQDIHEESLNESVKSEHSPRVVLWEIDLTVQGGERYFFCNELNE
                     KREPVTWQGRQYQAYPIEGSGFEMNGKGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMV
                     ANFGGFLSINKLSQ"
     gene            1086..1829
                     /locus_tag="ACHOT6_004764"
     CDS             1086..1829
                     /locus_tag="ACHOT6_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9949824.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGRNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            1826..2404
                     /locus_tag="ACHOT6_004765"
     CDS             1826..2404
                     /locus_tag="ACHOT6_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9949825.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
     gene            2645..>3096
                     /locus_tag="ACHOT6_004766"
     CDS             2645..>3096
                     /locus_tag="ACHOT6_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949826.1"
                     /translation="MGKGGGKGHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTE"
BASE COUNT          659 a          759 c         1044 g          634 t
ORIGIN      
        1 ctccggctgc agttgcgtga tggcggtatg ttatcaggga gcgggcgatg aaaacctttc
       61 gctggaaagt gaagccggat atggaggtga actcgcagcc atcggtgcgt gaagtgcgtt
      121 ttggtgacgg gtactcacag cgtatggcgg cagggctgaa tgctgacctg aaaacatacc
      181 gggtgacgct ttccgtgacc cgggaggagg cccggcatct ggaagcgttt ctggcagagc
      241 acgggggctg gaaggcattt ttgtggaagc caccctatgc ataccggcag ataaaggtga
      301 cctgtgccgg gtggtctgcg cgggtcggga tgttgcgcgt tgagttcagc gcggagttta
      361 agcaggtggt gaactgatgc aggacattca tgaagaaagt ctgaacgagt cggttaaatc
      421 agagcactca ccgcgggtgg tgctctggga aatcgacctg acggtgcagg gcggtgagcg
      481 gtattttttc tgcaatgagc tgaatgaaaa aagggagccg gtgacctggc aggggcggca
      541 atatcaggcg tacccgattg agggcagtgg ctttgagatg aacggaaagg gcagcagtgc
      601 ccgcccgtcg ctgacggtgt ccaatctgtt cggtcttgtc accgggatgg cggaggattt
      661 gcagagcctg gtgggtgcca cggtggtccg ccgccgggtg tatgcgcgtt ttctggatgc
      721 ggtgaacttt gtggcaggca atccggaggc cgacccggag caggagctga gcgaccgctg
      781 ggtggtggag cagatgtcag agctgacggc catgacagcc tcgtttgtgc tggcaacacc
      841 gacggagacg gacggagcgc tgtttcccgg tcgcatcatg ctggcgaaca cctgtatgtg
      901 ggattaccgg ggagatgaat gcggttatca cggtcctgcg gtggcggatg agttcgacaa
      961 ccccaccacg gatatccgga aggacagatg cagtaaatgc atgcgcgggt gtgagatgcg
     1021 cggcatggtg gctaatttcg gcggtttcct ttccattaat aaactttcgc agtaaatccc
     1081 ggtttatgac acagactgaa tcagcgattc tggcgcatgc ccggcggtgt gcgcctgcgg
     1141 agtcgtgcgg cttcgtgata ggcaccccgg agggcgaacg gtaccagccc tgcgtgaata
     1201 tctccgcaga gccggaggcg tattttcgta ttgcgccgga agactggctg caggcagaga
     1261 tgcaggggga gattgtggca ctggtccaca gccaccctgg tggtctgccc tggctgagcg
     1321 aggccgaccg gcggctgcag ataaagagtg ccctgccctg gtggctggtc tgccgggggg
     1381 aaattcacaa attccgctgt gtgccgcacc tgaccgggcg tcgttttgag cacggggtga
     1441 cggactgtta caccctgttc cgggatgcat accatctggc ggggataacg ctgccggatt
     1501 ttgtgcgtga ggatgactgg tggcgcaacg gtcggaacct ttacctggac aatatggcgg
     1561 cgactggttt ttaccgggtg cccctgtcct ctgcacaggc gggcgatatc ctgctgtgct
     1621 gctttggcgc atcggtggcc aatcatgccg ccatatactg cggcaacggt gaactgcttc
     1681 accatctgcc tgaacaactg agtaaacggg agaggtattc tgaaaaatgg caacgacgaa
     1741 cgcattctgt ctggcgtcac cgccactggc acgcatctgc cttcacgggg atttgcaacg
     1801 atttggccgc cgcctcagcc tgtacgtgaa cacggcagcg gaagccatcc gtgccctgtc
     1861 gctgcagatg ccgggattcc gccgtcagat gaacgaaggc tggtaccaga tacgtattcg
     1921 cggtgaggac acggcaccgg aggcggtgta cgcccgtctt cacgaacctc tgggtgaggg
     1981 ggcggtcatc catattgtgc cgcgactggc cggagccgga aagggtggac tgcagattgt
     2041 gctgggggcg gcagccatcg tgggctcttt cttcactgcc ggggcatcaa tggcgttatg
     2101 gggttcagcc ctggcagccg gtggtttttc tgccaccacg atgctgtttt ctctgggggc
     2161 cagcatgatt ctgggtggtg tggcccagat gctggccccg aaggcaaaaa caccggatta
     2221 ccgcgcaacg gataacggca gacagaacac gtacttttcg tcactggaca acatgattgc
     2281 ccagggtaac ccgatgccgg tgccttatgg tgaaatgctg gttggttcac gacggatatc
     2341 ccaggacatc agcacccgtg atgagggcgg agacgggaaa gtggtggtta tcgggcgggg
     2401 atgaaaataa aaaaatcccg cagagttagc ggagctgcgg gagagaacga tgaagattaa
     2461 cgttatggag ttatttttca ggcatcaaaa aagtaacgca gcgtcattat tgcggctaca
     2521 ggcaattgcc ggaaatgtga agagtttcag aaattttatt ccgtcatgac acaggcaccc
     2581 tccggggtgc ctgttgtttt ctggcataaa cagattcaga catcagacag gagaggggga
     2641 cagagtgggt aaagggggcg gcaaggggca cacgccggta gaggcaaagg acaatcttaa
     2701 gtccacgcag atgatgagcg tgattgatgc gattggtgag ggaccgatag aaggcccggt
     2761 gaagggactg cagagtatcc tggtgaacaa aaccccgctg acggacacgg acggtaatcc
     2821 cgtgatacac ggtgtgactg cggtctggcg cgccggggag caggagcaga caccaccgga
     2881 aggctttgag tcctccggag ctgaaaccgc actgggcgtg gaagtgacga aggcaaagcc
     2941 ggtgacgcgc accattacgt ccgcgaacat tgaccgcctg cgggtcacct tcggggtgca
     3001 gtcactgttg gagaccacct caaagggcga ccgtaatccc tcttctgtcc gactgctgat
     3061 tcagttgcag cgtaacggta actgggtgac ggaaaa
//
LOCUS       DAZKIV010000280         3046 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZKIV010000280 DAZKIV010000000
VERSION     DAZKIV010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.282"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHOT6_004767"
     CDS             complement(26..1885)
                     /gene="gss"
                     /locus_tag="ACHOT6_004767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFP9949827.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2090..2956
                     /gene="yghU"
                     /locus_tag="ACHOT6_004768"
     CDS             2090..2956
                     /gene="yghU"
                     /locus_tag="ACHOT6_004768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFP9949828.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDATEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          720 a          819 c          772 g          735 t
ORIGIN      
        1 cagtacgggg ttgctacaac atgtattact ttttcaccac aattaacggt tcaatatcac
       61 tctctttttt gatgaccagt gattcatcac cgcgcaaaca cgtcccaccg tagttgccgc
      121 caacggtgaa ggtacatacc tgaatgtatt taccgtccac tttcggcaaa caccacagtt
      181 gctgatagat gtttttctgc tcggcaaatt taccgctggt tttgtccagc acctcttcat
      241 gatggctgac caggtcgata ttgctaccac agcgaccggc aatcggtttc actgcgtaac
      301 ctgttttcac caattcatca ttaacagtga aatcggtatc cagcaggtaa cgatggtgcg
      361 ggaacagcga ccagaggatc ggcagaatcg ctttgttgcc ggggatcacc gtccacagcg
      421 gctcaaagac tagcacttcc gggcgcagca atacgtcgat aagacgcact tcgttttgcg
      481 gatgaccggt acggattggc accgcagcaa actcacggtc gctaacttca cgaatctgat
      541 caaacgcggt ttcccacgcc caggttttcc acacgcagtt aaccagtcgc ccttccccat
      601 caatcagttg cccggcagca tcccagccca gttcatccaa tccacgcaag atacgcgttt
      661 caaagcccgc ctggtgcagc gcctgctcca taaactgcgc gtgatagttt tcctcgatat
      721 ctttgtcctg catgatatgg acaaacggac gtgcacgact gtgtttccag gcaccagcca
      781 attcgttaat cagcccttcc gccggattga agccgttgcc tttatagccc tgctccgccc
      841 aacgttcgag gatcaagcca gcttcggtat gacaggaggc ggagtcggcg ttgtattcgt
      901 aaaccttcag gccacgctca tccatgcaga aatccatacg accagtaatc atatggtgac
      961 ggcgacgctg ccaggagaga cgcaaacgtg gccagaggat tttcgggatg tcgaacagcg
     1021 ccagcaggtt gtcatctttc agcaccttgt cggttgcgtg aagatacatc aggtgcagct
     1081 cgttggtggc tttaattagc tcctgctcgg cactctcggt aatggtgtag taatgataag
     1141 gatcctgatt gatcacctga ccgttggcct gcacataggc gttttgcagc ggatcttttt
     1201 catccagcca tttaccgtca aactggcctt tgttttccag gcgcgctccg ctgattttca
     1261 gcagctcgcc tgcaatttcc ggctgcggta agctgtattc agtatcttcc gtctggatca
     1321 tccagcccag aatggtggtg tcatcaaaag tgtctttcag ggtatagcag ccgttttcga
     1381 ccaccatctc cagctcacgc gtccactgtt gcccttgcgg caacggggaa tgaatcacgt
     1441 tctgttccgc aatacggact ttgttgccat gcaattgggt aatgatggcg acatggccag
     1501 tgtctttaaa ttcaccgcct ttatcccaga taagaagcgc acccgcgacc ggcgcacgcg
     1561 gcgagccgtt aggaaatgcc tgcaatggca ggatgttgtc attaaccact tctcgcagga
     1621 agcgcagcga gaaaatctcc cacgccatac ccacgtcagt aaagaccaca ccgtaattca
     1681 gaaagagaaa acggcgagca aattcaacgc attgccactt gtggcccata tattcgtcgt
     1741 cgatatagct acggaatacg gcgtcatctt cgtattcctg cggatcgaga gaactgtaat
     1801 ctgaagagta gattgctacc ccacctgggg cgtagcccaa taatgtcccg aacggggcat
     1861 cctggctggt cgttcctttg ctcatcactt tacctttaac aatacaacct aagcagttgg
     1921 cgtaattgtt gtgctctgat tacctgctca gcgacattaa ccggacagag gtcagactaa
     1981 tcatacacct catcgcagcg gctggcgcaa agggttaaaa aattcacatt ctgtacagca
     2041 agtgacctgc tacactgctt caacactacc actcagaagg caactcacta tgacagacaa
     2101 tacttatcag cccgcgaaag tctggacgtg ggataaatcc gctggcggcg cgttcgccaa
     2161 tatcaatcgc ccggtttctg gtccgacgca tgaaaaaacg ctgcccgttg gcaaacaccc
     2221 attgcaactt tattcgctgg gaacgccgaa cggtcagaaa gtaacgatta tgcttgagga
     2281 gctgctggcg ctgggcgtta ctggtgcaga gtacgacgcc tggctgattc gtattggcga
     2341 tggcgatcaa ttctccagcg gctttgtcga agtgaaccca aactcgaaga tcccggcgct
     2401 gcgcgatcat acgcataatc cgccgatccg cgtgtttgaa tctggttcga tcctgcttta
     2461 tctggcggag aaatttggct acttcctgcc gcaggatttg gcaaagcgta ctgaaacgat
     2521 gaactggctg ttctggttac agggcgcggc accgttcctc ggcggtggtt ttggtcactt
     2581 ttaccattac gcgccgatga aaattgagta cgccatcaac cgctttacca tggaagccaa
     2641 acgtctgctc gacgtgctgg ataagcaact ggcgcagcat aagtttattg cgggcgatga
     2701 gtacaccatt gcggatatgg cgatttggcc gtggtttggc aatgtggtgt taggcggcgt
     2761 gtatgatgcc acagagtttc ttgatgcggg cagttataag catgtacaac gctgggcgaa
     2821 agaagtaggc gaacgtccgg cggtgaaacg tgggcgtatt gttaaccgca ccaacggacc
     2881 gctgaatgag cagttgcatg agcgccatga cgccagtgat ttcgagacga atacggaaga
     2941 taagcgtcag gggtaagggt tggtgttcgt cgcagcaagc catccaggcc ggataaggcg
     3001 ttcacgccgc atccggcaat cgtgcacaat gcctgatgcg acgctg
//
LOCUS       DAZKIV010000281         3017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZKIV010000281 DAZKIV010000000
VERSION     DAZKIV010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.283"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..532)
                     /locus_tag="ACHOT6_004769"
     CDS             complement(<1..532)
                     /locus_tag="ACHOT6_004769"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
                     /protein_id="HFP9949829.1"
                     /translation="MRRTFTAEEKASVFELWKNGTGFSEIANILGSKPGTIFTMLRDT
                     GGIKPHERKRAVAHLTLSEREEIRAGLSAKMSIRAIATALNRSPSTISREVQRNRGRR
                     YYKAVDANNRANRMAKRPKPCLLDQNLPLRKLVLEKLEMKWSPEQISGWLRRTKPRQK
                     TLRISPETIYKTLYFRS"
     gene            594..1490
                     /locus_tag="ACHOT6_004770"
                     /pseudo
     CDS             594..1490
                     /locus_tag="ACHOT6_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            1441..2142
                     /gene="traT"
                     /locus_tag="ACHOT6_004771"
     CDS             1441..2142
                     /gene="traT"
                     /locus_tag="ACHOT6_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFP9949830.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFEEAK
                     PHLEDQLAKSIANIL"
     gene            complement(2190..2441)
                     /locus_tag="ACHOT6_004772"
     CDS             complement(2190..2441)
                     /locus_tag="ACHOT6_004772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9949831.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
     gene            complement(2465..2755)
                     /locus_tag="ACHOT6_004773"
     CDS             complement(2465..2755)
                     /locus_tag="ACHOT6_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFP9949832.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICSRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
BASE COUNT          844 a          734 c          683 g          756 t
ORIGIN      
        1 ggctacgaaa gtacagcgtt ttataaattg tctcaggtga tattcgcagc gttttttgac
       61 gtggttttgt tcgccttaac catcctgata tttgctctgg agaccatttc atctccagct
      121 tttccagaac aagctttcgc aatggtaaat tttgatccag taagcacggt tttggccttt
      181 tcgccattct gttggctcgg ttattagcat caacagcttt gtaatagcgt ctgccccgat
      241 tacgctgaac ttcacgtgag atcgtcgaag gactgcgatt cagcgcagta gctatcgcac
      301 gaatgctcat tttggctgac aaaccagctc gtatctcctc gcgctcagac agtgtcaggt
      361 gagctacagc ccgcttacgc tcatggggtt ttatgccgcc agtatccctt aacatagtga
      421 agatcgttcc gggttttgaa cccaggatat tcgctatttc actgaagcct gttccgttct
      481 tccatagttc aaaaacagag gctttttcct ctgctgtaaa tgttcgtctc attcaaaaaa
      541 cctccgcaac cccatgtttt cacataactg ttgcgttgac caattgaatc tacaacagcg
      601 tattttgcac aggagtcgct gaaaaatacg cgttaatcga acaatggcga caacaatttc
      661 ccattgaagc gatgtgtcag gtatttggtg tatccaggag cggttattac aactgggtac
      721 agcatgaacc ctcagacaga aaacaaagtg atgagcggct aaaactggag attaaggtgg
      781 cacatatccg cactcgcgaa acatatggaa cccggcggct ccagacggag ctggcagaga
      841 atggcatcat cgttggtcgt gaccgactgg cacgtcttcg taaggagcta aggctacgct
      901 gtaagcagaa acgcaagttc agggcgacta cgaactcgaa ccacaatctg ccagttgcgc
      961 caaatctgct gaaccagacg ttcgctccta cagcaccaaa tcaggtctgg gtggcggacc
     1021 tgacgtatgt tgccacacag gagggatggt tgtacctcgc tggcatcaaa gatgtttata
     1081 cgtgcgaaat tgtcggctac gccatgggag agcgcatgac aaaagagctg acaggtaaag
     1141 ccctgtttat ggcgctcagg agccagcgcc cacctgccgg gctaatccac cactctgatc
     1201 gaggttcaca gtactgcgca tacgattacc gggtcataca ggagcagttt ggtctgaaaa
     1261 catcaatgtc gcgtaaaggt aactgttacg acaacgctcc gatggaaagc ttctggggaa
     1321 cgctgaaaaa tgagagcctg agccactatc gttttaagag ccgggatgag gccatctcag
     1381 taatacggga atacattgag attttctaca atcgtcagcg tcgtcactct cgtctgggga
     1441 atatctcccc ggcagccttc agggaaaaat atcatcagat ggctgcttaa aaaaagaaca
     1501 aatggtagtg tccgctattg tcagtacacc tcaaccatct ggcttgaacc tgcgagcgaa
     1561 cgcaccgttt tcctgcagat caaaaacacg tcggataaag acatgagtgg actacaggga
     1621 aaaattgccg atgcagtcaa agccagaggt tatcagatca tgacttcccc ggataaagct
     1681 tactactgga tccaggctaa cgttctcaaa gcagacaaaa tggatctgcg tgaatctcag
     1741 ggatggctga gtcgcggtta tgaaggtgca gcagtcggag ctgctcttgg aggtggaatc
     1801 acagcgtaca ataccgggtc ggcaggtacc actctcggtg ttggactggc taccggtctg
     1861 attggcatgg ccgcggatgc aatggtggaa gatataaatt acacaatgat tacagatgtt
     1921 cagattgccg agcgaaccag aaccagcgtc agaacggata atgttgcagc tttgcgtcag
     1981 ggaacatcag gctccaaaat tcagacaagc acagaaaccg gcaaccagca taaataccag
     2041 acccgggttg tatccagtgc caaccaggtt aatctgaagt ttgaagaagc taaacctcat
     2101 ctggaagacc agctggcaaa atcaatcgcc aatatactct gatataaata tcccggtggc
     2161 cattcacctg gcctccggcc ctcttagtct caacgttcag acaattcaga tattattttc
     2221 cctgtcacac catataccca tgccggaaca gaatctaatg acggtaccag taaattcttc
     2281 agcgttcggt ttgcctgacg cccgtagctg tatttgtgtc tgtttcttct tatacaagag
     2341 cagtagtaca atttctcttc cagagtcagt tcaactttag gacgtaacca ataaagatct
     2401 cttccactat aaaatggtga atcctgtaca aacgattcca taacagaacc tcctgctgct
     2461 acagttaaca cagctgctgg catggactaa taccatcttt taatttaacc ctggcggaga
     2521 cgccattatt tttggacgtt cttgaaacaa aattaattcc tgaagaatct ttctccagtt
     2581 tattcagctc aagattcagt ccataattaa cgtcaaataa ctctgataaa ggtatcagtt
     2641 tatgttcagg ataatgatta tcccgacgag aacaaatatt aaaatcctgc atattacccg
     2701 acatcaggag ttcaaaaccg tccccggcat cattcatact ggcagataaa ttcattaccc
     2761 agtttagtgc taatcaacag gtacggctta acttacagga attccccggg aggggcggac
     2821 tcatcctcag ttagcggcaa aatcgcatca cagccaatcg cctgtctcga ttccccatcg
     2881 gcgaacaatg tatgtggcca caaaatcctg gctggctcag aacgaagagc atctgagcaa
     2941 agaggtcatc aatctggtat gaatatctgg cagaaaatat cccctgaatc cacaactgct
     3001 gtgcatatct gctcaaa
//
LOCUS       DAZKIV010000282         3006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZKIV010000282 DAZKIV010000000
VERSION     DAZKIV010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.284"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..304)
                     /locus_tag="ACHOT6_004774"
     CDS             complement(<1..304)
                     /locus_tag="ACHOT6_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949833.1"
                     /translation="MSEGPGGPQGATAGGTLAMRMLSQQAMVASQMKRAANDKAIAQM
                     LASKKSGPPAARLGDEIQHKSFLGALAGAVLGAIVTIAEGCLIMAACATGPYALVLV"
     gene            complement(324..785)
                     /locus_tag="ACHOT6_004775"
     CDS             complement(324..785)
                     /locus_tag="ACHOT6_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFP9949834.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(813..2714)
                     /locus_tag="ACHOT6_004776"
     CDS             complement(813..2714)
                     /locus_tag="ACHOT6_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFP9949835.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT          617 a          863 c          788 g          738 t
ORIGIN      
        1 gcaccagaac cagcgcataa gggccggtgg cacaggcggc cataatcagg caaccttctg
       61 cgatggtcac tatcgccccc agcacggccc ctgccagcgc ccccaaaaaa ctcttgtgct
      121 gaatttcatc gcccagcctg gcggcggggg ggccggactt ttttgatgcc agcatctgtg
      181 caatggcttt gtcgttggct gcccgtttca tctggctggc gaccatcgcc tgctgtgaca
      241 gcattcgcat tgccagcgta ccgcctgcgg tcgctccctg tggcccgcct ggtccttcac
      301 tcatgtgtta tccctcgttt atgtcagcgc ggctggtcct tttggatgtc attctccggt
      361 ggcaacgggg tgaagctctt tatcacctcc agcagcatcg tcttttgact gttcttcagg
      421 gataaggagg tgctgaaagt gaaggtcagc agcgtatctc cacgccgctg gaatacggct
      481 atctggctaa tccgcccctg attcccctca tcttccggac cgttccagga ataatccagc
      541 agccaggccg gggcctctgc cagagtgatt tcccggcagg catgttcggt gtacccatca
      601 agtttgccct ggtacagcgc cagcgtttcc tggtacagct ctggtgtcag ggttccctgt
      661 ggaacggctt cgcgggtaat gaccaggttg atgccactgt tatctgtgga caccaggacc
      721 tgcattgact gatcctgcca ttgagacggc agttgaatgt ggccttcatt aaaagtgaac
      781 tgcataaaat actccctgat aatctgagtg aatcagttga tgaaaacaga accgcccacg
      841 atggtgatgc catccttcga cagcctgatt accgtcgatt ccccgacctg tagctgaatg
      901 ccatccggcg tcagcgcaat tttgctttcc tccacggcca gcaaaatttc cttaatcgcc
      961 gtataagcct gtataccgtc gccttcgcca ccattgttta cagcgtcaac atgcaccgaa
     1021 ccggcaatcg tgcctctgtc ctgaaagaca tcctcagata aagacccttt cagtactccg
     1081 gtgatcttgt ggcctttttc caccgtaaga aggcggttac cttcctggat cgtgaccgtc
     1141 tggtcatttt ttacggtttc gcggtgatcg tgttttacgg tggtggtacg gtcgttgagc
     1201 acttcggtat ccatgttctt ctgcgcgtgg atgtagacct gttctctccc tgtcgcatca
     1261 tcaaacttca gctcattaaa tccgctgccc atgtacgttt tggagcggat ggtcatctgc
     1321 gtctttgttc ccggcaggct gccggggggg cggttttcct ggtggtaggt gcgccccata
     1381 atgatcggct ggtccggatc gccgttgagg aagtctacaa tcacctcctg acccacacgc
     1441 ggtatcgcca ggtggccaaa accggtgcct gcccacgcct gtgccacacg gatccagcac
     1501 gaactgtctt ggtctgccgg gttataacgg tcccagttga acttcacccg cacgcgacca
     1561 tgttcgtcgc agaagatttc ctcgcctgcc ggtcctgtca cgacagcgct ctgcgggccg
     1621 tcgaccagcg gtttcagcaa cggctgtggt cgccatgttc tgtctgccgg gatcaccgca
     1681 aaatggttct ccagcgccgt ccccgctccc tgccgtcctg gcaccgcctg tggctgttcg
     1741 ccgtgcagtt cacttgccac cacctgccat tcccggttca ggttcgcctg cggatgcccc
     1801 gtcagcacaa ttcgccgtcc cggccatatc tccggcgagc ggctcattcc ccgcgcggtt
     1861 tctgcgttgt ttcgccagcc gtccatctgc cagcgggcaa agttctgccc gtgggcgctc
     1921 ttgaaacgtc cggggtagtc atacacttca tactgcgtgc gctggtagtc ctggtgctgg
     1981 ccttcctgtt caaaacgtcc ggcccagccg gggcgtttaa aggtgtagtc tttggtcacc
     2041 acggaagaag ggcggatttg tgcgctgtag cggaactggc tgatgcagag ggtgctcacc
     2101 tcggtacggg tgttcgggtt ccatgggatt tcaaaagatt cgggcagatg gcggactgtg
     2161 tcgcacagca ccaggctctg gtcggtactt ttgtaagcat gctcctcata aaagaagatg
     2221 ccttcctccg ccgccatccg gcacaggaaa tcgtaatcag tctcaccgta ctggacacaa
     2281 aactcacggg aaggatgcgg ctcgctgaac agcggactcc attcggtcac cccgttttcc
     2341 tgcaacatcg tgccgaggat gcttttgatg tcctcgttct ggaagatacg gaagttctgg
     2401 cgcagaccgg cacgccacag cggcgggtgc accttcatgc tgtacagcat ctggtttttg
     2461 tcgttctccc cgagttcaaa ccaggtcacc acgcctttca cccggcgctg tacgtcatcg
     2521 ccctgccata tcgtcaggta ggccattttg tccagcacct gcgcaaattc aagggagaga
     2581 aactgctggc tgaccaggga gagatcgagg gaaaagagcg aagagagtga ctggttcaga
     2641 tgaaaggaaa ccaccgcaaa agcgtccggc ggcaggccgt ccacttccag tgtgaaacgt
     2701 aatccggttg acatagcaac ctccagtaaa atgaataatg atccgtaccg tacaactgcg
     2761 gataatgtga taagcgggac gacttcagac gttattaggg aaggaacaga aaatgctatg
     2821 gttatgtgag aattttgttt aaatattact ttacagaaac gggtgtctgt tcagtcaaaa
     2881 aataatcaca agctcaacct cttgtttctt ggcgttattc aggacaggta cgcatatcac
     2941 aacgagtaac aaaaacgcaa agttaaacca atattcataa tatattttta ttcacagcaa
     3001 taaata
//
LOCUS       DAZKIV010000283         2998 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZKIV010000283 DAZKIV010000000
VERSION     DAZKIV010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.285"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(138..214)
                     /locus_tag="ACHOT6_004777"
     tRNA            complement(138..214)
                     /locus_tag="ACHOT6_004777"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(178..180),aa:Asp,seq:gtc)
     gene            complement(347..1078)
                     /gene="dnaQ"
                     /locus_tag="ACHOT6_004778"
     CDS             complement(347..1078)
                     /gene="dnaQ"
                     /locus_tag="ACHOT6_004778"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFP9949836.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1143..1610
                     /gene="rnhA"
                     /locus_tag="ACHOT6_004779"
     CDS             1143..1610
                     /gene="rnhA"
                     /locus_tag="ACHOT6_004779"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFP9949837.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1607..2329)
                     /locus_tag="ACHOT6_004780"
     CDS             complement(1607..2329)
                     /locus_tag="ACHOT6_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFP9949838.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2363..>2998
                     /gene="gloB"
                     /locus_tag="ACHOT6_004781"
     CDS             2363..>2998
                     /gene="gloB"
                     /locus_tag="ACHOT6_004781"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFP9949839.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQ"
BASE COUNT          817 a          726 c          705 g          750 t
ORIGIN      
        1 gaatgagaca acaagcgaga atcacgcact tacgccagaa taaaatcatt gaacagagaa
       61 atggtggaat tcaaatacaa aaaaaccgca aaattaaaaa tcttgcggct ctctgaactc
      121 attttcatga gtgaatttgg cggaacggac gggactcgaa cccgcgaccc cctgcgtgac
      181 aggcaggtat tctaaccgac tgaactaccg ctccgcgttg tgttccgttg ggaacgaggc
      241 gaatattacg gattgcctca cccttcgtca acggtttttc tcatcttttg aatcgtttgc
      301 tgcaaaaatc gcccaagccg ctatttttag cgccttttac aggtatttat gcccgccaga
      361 ggcagcttcc gcccttcttc tccaccagat caagacgggc ttcatgagct gcaagctctt
      421 catctgtcgc aaaaacaacg cgtaacttac ttgcctgacg tacaatgcgc tgaattgttg
      481 cttcaccttg ttgctgctgt gtctctcctt ccatcgcaaa agccatcgac gtttgaccac
      541 cggtcatcgc cagataaact tccgcaagga tctgtgcatc gagtaatgcc ccgtgtagcg
      601 ttcgtttact gttatctatt tcgtagcggg cacataacgc atcgaggctg ttgcgcttac
      661 caggaaacat tttcctcgcc accgcaaggc tatcggtgac cttacagaag gtattggtct
      721 tcggaatatc gcgcttaagc aacgaaaact cgtagtccat aaagccgata tcgaacgctg
      781 cgttatggat caccaactcc gcgccgcgga tatagtccat gaactcatcg gctacttcgg
      841 caaacgtggg cttatcgagc aaaaattcat cggcaatacc atgtacgcca aaggcttccg
      901 gatccaccag ccgatcgggt ttgagataaa catggaagtt attgcccgtc aggcgacggt
      961 tcaccacttc aacggcacca atctcaatga tcttgtggcc ttcatagtgc gcaccaatct
     1021 ggttcatacc ggtggtttcg gtatcgagaa cgatctggcg tgtaattgca gtgctcatag
     1081 cggtcattta tgtcagactt gtcgttttac agttcgattc aattacagga agtctaccag
     1141 agatgcttaa acaggtagaa attttcaccg atggttcgtg tctgggtaat cctggacctg
     1201 ggggttacgg cgctatttta cgctatcgcg gacgcgagaa aacctttagc gcaggctaca
     1261 cccgcaccac caacaaccgt atggagttga tggccgctat tgtcgcgctg gaggcgttaa
     1321 aagaacattg cgaagtcatt ttgagtaccg acagccagta tgtccgccaa ggtatcaccc
     1381 agtggatcca taactggaaa aaacgtggct ggaaaacagc agacaaaaaa ccagtaaaaa
     1441 atgtcgatct ctggcaacgt cttgatgctg cattggggca gcatcaaatc aaatgggaat
     1501 gggttaaagg ccatgccgga cacccggaaa acgaacgctg tgatgaactg gctcgtgctg
     1561 cggcaatgaa tcccacactg gaagatacgg gctaccaagt cgaagtttaa gcttgtggtt
     1621 tacgacattg ccgggtggct ccaaccgcct ggcgaattcg tggcttgttt ttactctgtt
     1681 tcatcgggtt tagcgttaaa ggaatagtcc gtttccgggc aacaataagt tgtaagcaac
     1741 caagcgcagg aatatgcgca ttcaatagtt ttcctccgtg tttgttccac gggagaacgt
     1801 ggaaacggct ggcgtgcagc acttcaaaat tcaacaaaga gagccagtcc agttgccgca
     1861 tcagagtaaa catccggctg ttatagggcg aggtttttcg caataccggc acaagtttgc
     1921 gtaatcccat taaactaatg ggattgaagc cactaatcac cagccagcca tcatcaatca
     1981 atacccgatc ggtttcacgc aataaacgat gcggatcggt gcaccacggc aatgtatgtg
     2041 ccagtagaca aacatcaacg gatttatcgg caaaaggaag atgaagtgga tccgcctgca
     2101 cctggacggg cattccttgc gcagaaacat tcacttgatg agaaaccgcg cacgcttcgc
     2161 aattgatttc tgcgcttaaa ttgccaatct taagcagatg aaaaccatac attttagtga
     2221 accacgggtt gagctggcgc tccagcgcct tgcgataaag ctctccccag ggcaaatcgc
     2281 cccagcgatc aggagccacg acagtttgag ggactcttgc cggtttcatc acaaccttcc
     2341 gtttcacact aagaggtaat ctatgaatct taacagtatt cccgcctttg atgacaatta
     2401 catctgggtt ttgaatgatg aagcaggtcg ctgcctgatt gtcgatcccg gagacgcaga
     2461 gccggtatta aacgccattg ccgccaataa ctggcaaccg gaggccatat ttctcaccca
     2521 ccatcatcac gatcacgttg gcggcgtaaa agaactggtg gaaaagtttc cgcaaattgt
     2581 ggtgtatggt ccacaagaga cacaagataa gggaacaaca caggtagtca aagatggcga
     2641 aactgccttc gttttggggc atgaatttag tgtaattgcc acaccgggtc acactttagg
     2701 acatatctgt tacttcagca aaccttatct attttgcggc gacacgctgt tttctggtgg
     2761 gtgtggtagg ttgtttgaag ggacagcatc acaaatgtat caatcactta ataagttaag
     2821 tgcgttacct gacgatacat tggtatgttg tgctcatgaa tatacgttat caaatatgaa
     2881 gtttgctttg agtattcttc cgcacgattt gtccataaat gattattatc gtaaagttaa
     2941 ggagttacgg gcaaaaaatc aaataacact acccgtaatt ctgaaaaatg agcggcaa
//
LOCUS       DAZKIV010000284         2979 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZKIV010000284 DAZKIV010000000
VERSION     DAZKIV010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.286"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            47..523
                     /gene="agaB"
                     /locus_tag="ACHOT6_004782"
     CDS             47..523
                     /gene="agaB"
                     /locus_tag="ACHOT6_004782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFP9949840.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            562..1365
                     /gene="agaC"
                     /locus_tag="ACHOT6_004783"
     CDS             562..1365
                     /gene="agaC"
                     /locus_tag="ACHOT6_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFP9949841.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1355..2146
                     /gene="agaD"
                     /locus_tag="ACHOT6_004784"
     CDS             1355..2146
                     /gene="agaD"
                     /locus_tag="ACHOT6_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFP9949842.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            2147..2902
                     /locus_tag="ACHOT6_004785"
     CDS             2147..2902
                     /locus_tag="ACHOT6_004785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFP9949843.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          743 a          691 c          735 g          810 t
ORIGIN      
        1 tattaaaaaa aattatatta ttcaacttta tggtgaggat tacacaatga gcagtccaaa
       61 tattctctta acccgtattg ataaccgtct ggttcacggt caggttggcg tgacctggac
      121 atccaccatc ggtgcgaatc tgctggtagt cgtggatgat gttgtcgcta acgatgatat
      181 tcaacagaaa ttaatgggta ttaccgcaga aacctacggc tttggcattc gtttctttac
      241 tatcgaaaaa accattaacg tcatcggcaa agctgcaccg catcagaaga tcttcctgat
      301 ttgccgtacg ccacaaacgg tacgtaaatt ggtagaaggt ggcattgacc tgaaagatat
      361 caacgtcggc aatatgcatt tctcggaagg gaaaaagcaa atcagcagta aagtttatgt
      421 cgatgaccag gatctcgcgg acttacgttt tattaaacaa cgtggcgtga atgttttcat
      481 tcaggacgtc cctggcgatc aaaaagaaca aatccctgac taaatctaaa atcgccttaa
      541 tattggtttg aggtaataaa aatgcatgaa ataaccctac ttcagggatt atccctggcg
      601 gcgttagttt tcgttctggg gattgatttt tggctggaag ccttattttt attccgcccg
      661 ataatcgttt gtaccctaac tggcgctatt ctcggtgata ttcagactgg cttaattacc
      721 ggtggtctga cagagttggc tttcgccgga ttaacccctg caggtggtgt tcagccgccc
      781 aacccgatta tggcgggtct gatgaccacc gtcattgcat ggtctacggg cgttgatgcc
      841 aaaacagcaa ttggtcttgg cctgccgttt agtttgttaa tgcagtacgt cattctgttc
      901 ttctattccg ctttctcatt atttatgacc aaagccgata aatgcgcgaa agaggcggat
      961 acggcagcgt tttcccggct taactggaca acgatgctca tcgtcgcttc agcgtatgcg
     1021 gtgattgctt tcctctgtac ttacctggca caaggggcga tgcaggcgct ggtgaaagcg
     1081 atgcccgcct ggttgaccca cggctttgaa gtggctggcg gtattctgcc tgccgttggt
     1141 tttggcttgc tgctgcgcgt aatgttcaaa gcgcaatata tcccttacct gatcgccggt
     1201 ttcctgtttg tttgctacat ccaggtcagc aacctgttgc cggttgccgt actgggcgca
     1261 ggctttgcgg tgtatgagtt tttcaatgcg aaatcccggc agcaagcgca accgcagccc
     1321 gttgccagta aaaatgaaga agaggactac agcaatggga tctgaaatca gtaaaaaaga
     1381 tatcacccgt ctgggctttc gttcgtcgct gctgcaagcg agctttaact acgaaaggat
     1441 gcaggcgggc ggttttacct gggcgatgtt gccgatcctg aaaaagattt ataaggacga
     1501 caaaccgggc ttaagcgcgg cgatgaaaga taacctcgaa tttattaata cccacccgaa
     1561 tctggtcgga ttcctgatgg ggttattaat ttcgatggaa gaaaaaggag aaaaccgcga
     1621 caccattaaa ggcctcaaag tggcactgtt tggcccaatc gccgggattg gcgatgcgat
     1681 tttctggttt actttgttgc cgattatggc gggaatttgc tcatcatttg ccagccaggg
     1741 aaacctgctg gggccgattc tatttttcgc cgtttacctg cttatctttt tcctgcgcgt
     1801 cggctggacc cacgtcggtt attcagtcgg cgtgaaggcg atcgataaag tgcgagagaa
     1861 ctcgcagatg attgctcgct cggcaaccat cctcgggatc acggtaatcg gcgggctgat
     1921 cgcttcgtat gtgcatatta acgtggtgac atcgtttgcc atcgacagta cccacagcgt
     1981 cgcgctgcaa caggatttct tcgataaagt cttcccgaac attttaccga tggcctacac
     2041 cctgctgatg tattacttcc tgcgggtgaa aaaagcgcat ccggtgctgt taatcggcgt
     2101 gacttttgtg ctctctattg tttgttccgc attcggcatt ttgtaaatgg aacgaggcac
     2161 tgcttctggc ggtgcctctt tacttaagga atttcatcct gtgcaaaccc ttcagcaagt
     2221 tgaaaactat acggcgttaa gtgaacgagc cagcgaatat ttattggctg tgatccgtag
     2281 caaaccggat gccgtgattt gcctggcgac cggagccacg ccattactga cgtatcatta
     2341 tctggtagaa aaaatccacc agcagcaggt tgatatcagc cagctcacct tcgtgaagct
     2401 cgacgaatgg gtggatctgc cattaacgat gcctggcacc tgcgaaactt tcctacaaca
     2461 gcatatcgtg cagccgctgg ggctgcgtga agaccagctc atcagctttc gctccgaaga
     2521 gataaatgag acagagtgcg aacgggtaac gaacctgatt gcgcgcaaag gcggtctgga
     2581 tttatgcgtt ctaggattgg ggaaaaacgg tcatcttggg ctgaacgaac cgggagaaag
     2641 cctacaaccg gcctgccata tcagtcaact tgatgccaga acacagcaac atgagatgtt
     2701 aaaaaccacg ggtcgccccg tgactcgtgg gatcacctta ggcctgaagg atattctcaa
     2761 tgcccgcgag gttttgttat tggtgactgg cgaaggaaag caggatgcga cagaacgttt
     2821 tctcacggct aaagtctcca ccgctatccc ggcttcgttt ttatggctgc acagtaactt
     2881 tatttgttta attaatacct aaccagatga aaaatttgct caaaattttt aaattaactt
     2941 attcaacagt cacgcattat attaaataac atttgactg
//
LOCUS       DAZKIV010000285         2969 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZKIV010000285 DAZKIV010000000
VERSION     DAZKIV010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.287"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..180
                     /gene="gpU"
                     /locus_tag="ACHOT6_004786"
     CDS             <1..180
                     /gene="gpU"
                     /locus_tag="ACHOT6_004786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFP9949844.1"
                     /translation="PDSELDAWMESRIYPVMSDIPALSDLITSMVASGYDYRRDDDAG
                     LWSSADLTYVITYEM"
     gene            188..928
                     /locus_tag="ACHOT6_004787"
     CDS             188..928
                     /locus_tag="ACHOT6_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949845.1"
                     /translation="MPVPNPAIPVKGAGTTLWVYNGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASSSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAAVAEITVTDS"
     gene            944..1366
                     /locus_tag="ACHOT6_004788"
     CDS             944..1366
                     /locus_tag="ACHOT6_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9949846.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            1348..1782
                     /locus_tag="ACHOT6_004789"
     CDS             1348..1782
                     /locus_tag="ACHOT6_004789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9949847.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            1775..>2969
                     /locus_tag="ACHOT6_004790"
     CDS             1775..>2969
                     /locus_tag="ACHOT6_004790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9949848.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYT"
BASE COUNT          666 a          745 c          964 g          594 t
ORIGIN      
        1 ccggattcag agctggatgc gtggatggag tcccggattt atccggtgat gagcgatatc
       61 ccggcactgt cagatttgat caccagtatg gtggccagtg gctatgacta ccggcgcgac
      121 gatgatgcgg gcctgtggag ttcagccgat ctgacgtatg tcattaccta tgaaatgtga
      181 ggacgctatg cctgtaccaa atccagcaat accggtgaaa ggtgccggaa ccaccctgtg
      241 ggtttataac gggagcggcg acccttatgc gaacccgctt tcagacgttg actggtcgcg
      301 tctggctaaa gttaaagacc tgacgcccgg cgaactgacc gctgagtcct atgacgacag
      361 ttatctcgat gatgaagatg cggactggac cgcgaccggg caggggcaga aatccgccgg
      421 agataccagc ttcacgctgg cgtggatgcc cggagagcag gggcagcagg cgctgctggc
      481 gtggtttaat gaaggtgata cccgtgccta taaaatccgc ttcccgaacg gcacggtcga
      541 tgtgttccgt ggctgggtga gcagtatcgg taaggcggtg acggcgaagg aagtgatcac
      601 ccgtacggtg aaggtcacca atgtgggccg tccgtcaatg gcagaagatc gcagtacggt
      661 gacggcggca accggtatga ctgtgacgcc tgccagctcc tcggtggtga aagggcagag
      721 caccacgctg accgtggcat tccagccgga gggcgcaact gacaagagct tccgtgcggt
      781 gtcagcggat aaaacaaaag ccaccgtgtc ggtcagtggt atgaccatca ccgtgaaagg
      841 cgttgctgca ggtaaggtca acattccggt tgtatccggt aatggtgaac ttgctgcggt
      901 tgcagaaatc actgtcaccg acagttaatc cggagagtca gcgatgttcc tgaaaaccga
      961 atcatttgaa cataacggcg tgaccgtcac gctttctgaa ctgtcagccc tgcagcgtat
     1021 tgagcatctc gccctgatga aacggcaggc agaacaggcg gagtcagaca gcaaccggaa
     1081 gtttactgtg gaagacgcca tcagaaccgg cgcgtttctg gtggcgatgt ccctgtggca
     1141 taaccatccg cagaagacgc agatgccgtc catgaatgaa gccgttaaac agattgagca
     1201 ggaagtgctt accacctggc ccacggaggc aatttctcat gctgaaaacg tggtgtaccg
     1261 gctgtctggt atgtatgagt ttgtggtgaa taatgcccct gaacagacag aggacgccgg
     1321 gcccgcagag cctgtttctg cgggaaagtg ttcgacggtg agctgagttt tgccctgaaa
     1381 ctggcgcgtg agatggggcg acccgactgg cgtgccatgc ttgccgggat gtcatccacg
     1441 gagtatgccg actggcaccg cttttacagt acccattatt ttcatgatgt tctgctggat
     1501 atgcactttt ccgggctgac gtacaccgtg ctcagcctgt ttttcagcga tccggatatg
     1561 catccgctgg atttcagtct gctgaaccgg cgcgaggctg acgaagagcc tgaagatgat
     1621 gtgctgatgc agaaagcggc agggcttgcc ggaggcgtcc gctttggccc ggacgggaat
     1681 gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg aggatgacgt aatgctgatg
     1741 acagtatcag aagggatcgc aggaggagtc cggtatggct gaaccggtag gcgatctggt
     1801 cgttgatttg agtctggatg cggccagatt tgacgagcag atggccagag tcaggcgtca
     1861 tttttccggt acggaaagtg atgcgaaaaa aacagcggca gtcgttgaac agtcgctgag
     1921 ccgacaggcg ctggctgcac agaaagcggg gatttccgtc gggcagtata aagccgccat
     1981 gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag cttgcaggcg ggcaaagtcc
     2041 gtggctgatc ctgctgcaac agggggggca ggtgaaggac tccttcggcg ggatgatccc
     2101 catgttcagg gggcttgccg gtgcgatcac cctgccgatg gtgggggcca cctcgctggc
     2161 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
     2221 caacaaaacg ctggtccttt ccggcaatca ggcgggactg acggcagatc gtatgctggt
     2281 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
     2341 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
     2401 ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag gtcgcagaag ccttcgggaa
     2461 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
     2521 ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
     2581 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
     2641 gaacatgggc acgctggaga cctgggcaga caggactgcg cgggcattca aatccatgtg
     2701 ggatgcggtg ctggatattg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
     2761 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt attttgttaa
     2821 cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc
     2881 cgcccgaaag aaggctgagc agcagactca acaggacaaa aatgcgcagc agcagagcga
     2941 tactgaagcg tcacagctga aatataccg
//
LOCUS       DAZKIV010000286         2961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZKIV010000286 DAZKIV010000000
VERSION     DAZKIV010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.288"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            147..419
                     /locus_tag="ACHOT6_004791"
     CDS             147..419
                     /locus_tag="ACHOT6_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949849.1"
                     /translation="MAHNTKNHNSDAVRASRHGRRSRVFKTDWYQHPPCTEEQAEWLI
                     QCYRRHGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            421..1485
                     /locus_tag="ACHOT6_004792"
     CDS             421..1485
                     /locus_tag="ACHOT6_004792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9949850.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRVLVGPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNIALPPHVHQHQQQNPPQEKTVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQ
                     KCMTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHSELHAGVKDFEEKHGSQL
                     LLLIRFLMHARNSGVLKWKA"
     gene            1486..1866
                     /locus_tag="ACHOT6_004793"
     CDS             1486..1866
                     /locus_tag="ACHOT6_004793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9949851.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRHGNTYFISEAGKRYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKALLDALTHAGLLIDDEQFDEINIVR
                     GLPVPGGRLGVKIYEITHGWQAKE"
     gene            1863..2684
                     /locus_tag="ACHOT6_004794"
     CDS             1863..2684
                     /locus_tag="ACHOT6_004794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFP9949852.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLAEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEIFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR"
BASE COUNT          766 a          699 c          811 g          685 t
ORIGIN      
        1 ccctcgccac ctctgatgtg tcaggcatcc tcaacgcacc cgcacttaac ccgcttcggc
       61 gggttttgtt ttttcctggc attctggttt acaattcgca cgccagcctg aacaactggc
      121 acctgctgcg ccagcagaga aaacaaatgg cgcacaatac caaaaatcac aattctgatg
      181 ccgttcgtgc cagcaggcat gggcggcgtt ctcgcgtatt taaaaccgac tggtaccaac
      241 accctccatg cactgaagaa caggccgaat ggctgattca gtgttaccgc agacacggat
      301 acgagattaa gaaagccctc agcctcgatt atcgtcactg gataatctcc gtcaggcttc
      361 cttactccga acgcccaccg cgtccgtccc gcacattcca gcaacggatc tggaggtaac
      421 gtgcgggtat tacttcgacc tgttctggta ccggaactcg ggctggtgat cgttaagccg
      481 ggccgtgaat ccatgccggt attccacaat acccgggtat tggtggggcc ggaaccgaaa
      541 agcatgcgta atctgccgtc cggggtcgtt cctgccgttc gccagccgct ggtggaagac
      601 aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg tggtgctggt
      661 gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca cggcgattat
      721 catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt gtgctggcac
      781 tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc tcatcaaaac
      841 ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca ggagcgtgaa
      901 ttatctttgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga cgcgctaccg
      961 gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaatccgctc aatgtaccgt
     1021 gaaagcgaca tcgtaccggg agagcagacc gccaccagca tactgaagca gcgcacaaaa
     1081 aatattgcgc taccgcctca cgtccaccag caccagcaac agaacccacc acaggaaaag
     1141 acggtggtca gcattgccgt tgatccggaa tctccggctc agtatctcca gcgccagaaa
     1201 ccacaacggg aagagatgcc tgtatacacg cgctgggtaa aaacgcagaa atgcatgaca
     1261 tgcggtaatc aggcagatga tccgcatcac atcattggtc atggactggg agggatggga
     1321 acaaaggctg atgatttgtt tgttataccg ctgtgccgta aatgccatag cgaactacac
     1381 gccggggtaa aagattttga agaaaaacac ggcagccagc tgttgttgct gattcgtttt
     1441 ttaatgcacg cgagaaattc gggtgtcctg aagtggaaag catgaatgac tgaacgcata
     1501 gaattcgttt tgccttaccc gccgacggtg aatacctact ggcgacgtca tggcaatacg
     1561 tatttcatct cggaagccgg aaagcgttat cgccgtgatg tggcgctaat tgttcgccag
     1621 cagcgactga aattaaacct gtccggaagg ctggcgataa agattattgc agagccaccg
     1681 gataagcgcc gtcgtgacct ggacaacatt ctgaaagcgc tactggatgc gctgacgcat
     1741 gcaggactac ttatcgacga cgagcagttt gatgaaatta atattgtgcg cggtctgcct
     1801 gttccaggcg gacggcttgg tgtgaaaatt tacgaaataa cgcatggctg gcaggcaaaa
     1861 gaatgaaact ggaagattta ccaaaatact attccccaaa atcaccgggt ctgacggatg
     1921 catcggcctc aacgtcaaaa gatgcgctga gtatcactga tgtgatggca gcgcagggca
     1981 tgacacagaa tcgggctgag atggggtttt ctgcgttcct tgggaaaatg ggcatcagta
     2041 tgaatgacag ggagcgggca acagaactgc tggcagaata cgctctcagt cggtgtgatc
     2101 gcgtggcagc gttaagaaaa cttccggcag aaataaaacc ggtagtgatg cgcattatgg
     2161 cttcgtatgc gtttgaggat tatgcccgga gcgcggcgag caaaaaacag tgcccatgct
     2221 gtcacggaaa aaaatttatt gaaagcgaga tttttacaaa caagattcag tatccggatg
     2281 gtaagccgcc agtatgggca aagtgtacaa aaggcgtgta tccgtcttac tgggaggaat
     2341 ggaaaaaagt cagggaggtg gtaaaagttg cctgtccgga gtgtggaggg aagggggagg
     2401 tttccaccgc ctgtaaagat tgtcgtgggc gcggtgttgc cattcatcgt gaagagtcgg
     2461 taaaacgtgg tatgcctgtt atcagagact gccagcgttg tggtggtcgt ggctatgaaa
     2521 gattaccttc aacggaggca tttaatgcca tatgtaatgt aaccgatgcc atatctcttg
     2581 atacatggaa aaaaacagtt aaacgttttt acgatacgct ggtggtgcag tttgatattg
     2641 aagaagcatg ggcagaacaa caactgaaaa aggtgaccag atagctttgt tgatttttcc
     2701 cgaatctgtg gtaaatttgc cctaacgatg ggcgttttat gcctgacgtt agaagatttt
     2761 ttacacccgt cgccaggcgg gtttttttat gactgaaatc acgccagtac agtaaacgcg
     2821 ctggtggttg tgaataccgg tctttcagct tgctggcttt ttcgacaaga gttattggtg
     2881 tgtcacgtta accggaaaaa ggaaagtttg agaaacgcga tctggcacag gcggtgatta
     2941 acgccgcata cctggtggcc t
//
LOCUS       DAZKIV010000287         2939 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZKIV010000287 DAZKIV010000000
VERSION     DAZKIV010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.289"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..304
                     /locus_tag="ACHOT6_004795"
     CDS             <1..304
                     /locus_tag="ACHOT6_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFP9949853.1"
                     /translation="DRWVVEQMSELTAMTASFVLATPTETDGALFPGRIMLANTCMWD
                     YRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            315..1058
                     /locus_tag="ACHOT6_004796"
     CDS             315..1058
                     /locus_tag="ACHOT6_004796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9949854.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGDIHK
                     FRCVPHLTGRLFEHGVTDCYTLFRDAYHLAGIDMPDFEREDDWWRNGQNLYLDNMEAT
                     GFYRISLPSAQPGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSAWRHRHWHVSAFTGIYNDLAAASACM"
     gene            1055..1636
                     /locus_tag="ACHOT6_004797"
     CDS             1055..1636
                     /locus_tag="ACHOT6_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9949855.1"
                     /translation="MNTAAEAIRALSMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAGGFS
                     ATTMLFSLGASMILGGVAQMLAPKAKVPEYKSTDNGRQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRQA"
     gene            complement(1827..2354)
                     /gene="sodC"
                     /locus_tag="ACHOT6_004798"
     CDS             complement(1827..2354)
                     /gene="sodC"
                     /locus_tag="ACHOT6_004798"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFP9949856.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
     gene            2488..>2939
                     /locus_tag="ACHOT6_004799"
     CDS             2488..>2939
                     /locus_tag="ACHOT6_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949857.1"
                     /translation="MGKGGGRAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTE"
BASE COUNT          649 a          722 c          912 g          656 t
ORIGIN      
        1 cgaccgctgg gtggtggagc agatgtcaga gctgacggcc atgacagcct cgtttgtgct
       61 ggcaacaccg acggagacgg acggggcgct gtttcctggt cgcatcatgc tggcgaacac
      121 ctgtatgtgg gattaccggg gagatgaatg cgggtataac ggtcctgcgg tggcggatga
      181 gttcgacaac cccaccacgg atatccgtaa ggacagatgc agcaagtgca tgcgcgggtg
      241 tgagatgcgc ggcatggtgg ctaattttgg cggtttcctt tccattaata aactttcgca
      301 gtaaatcccg gtttatgaca cagactgaat cagcgattct ggcgcatgcc cggcggtgtg
      361 cgcctgcgga gtcgtgcggc ttcgtggtga gaacgccgga gggggagcgg tatatccctt
      421 gtgtgaatat ctctgcagag ccggaggcgt attttcgtat tgcaccggaa gactggctgc
      481 gggcagagat gcagggggag attgtggcac tggtccacag tcatcccggt ggtctgccct
      541 ggctgagcga ggccgaccgg cggctgcaga taaaaagtgc actgtcctgg tggctggtct
      601 gccgggggga cattcataaa ttccgctgtg tgccacatct gacaggacgg ctctttgagc
      661 acggggtgac ggactgttac acgctgttcc gggatgcata ccatctggcg ggaattgata
      721 tgccggattt tgagcgtgag gatgactggt ggcgcaatgg tcagaacctg tacctggaca
      781 atatggaggc gactggtttt tacaggattt ccctgccttc cgcacagcct ggcgatatcc
      841 tgctgtgctg ctttggcgca tcggtggcca atcatgccgc catatactgc ggcaacggtg
      901 aactgcttca ccatctgcct gaacaactga gtaaacggga gaggtattcc gaaaaatggc
      961 aacgacgaac gcattcagcc tggcgtcacc gccactggca cgtatctgcc ttcacgggga
     1021 tttacaacga tttggccgcc gcctcagcct gtatgtgaac acggcagcgg aagccatccg
     1081 tgccctgtcg atgcagatgc cgggattccg ccgtcagatg aacgaaggct ggtaccagat
     1141 acgtattgcc ggtgatgaca cggcaccgga ggcggtgtac gcccgtcttc acgaacagct
     1201 gggtgaggga acggtcatcc acattgtgcc gcgactggcc ggggccggaa agggtggact
     1261 gcagattgtg ctgggggcgg cggccatcgt ggggtcgttc ttcactgccg gggcatcaat
     1321 ggcgttatgg ggttcagccc tggcagccgg tggtttttct gccaccacga tgctgttttc
     1381 acttggagcc agcatgattc tgggtggtgt ggctcagatg ctggccccga aggctaaagt
     1441 accggagtac aaaagcacgg ataacggcag acagaacacg tacttttcct cgctggataa
     1501 catgattgcc caggggaacc cgatgccggt gccttacggt gaaatgctgg ttggctcccg
     1561 gcgaatctcc caggacatca gtacccgtga tgaaggcggt gacgggaagg tggtggttat
     1621 cgggcggcag gcataaaagc gaaaaaatcc cgcagtgctc acggacagga actgcgggag
     1681 cgttacgaag attgagtgta aggaattatt cttatgtcac gacaaaaaca ttaactcaga
     1741 gagggaggat gtgccgttct tttcagggag aaaggattta tcgtcctgag gaataaaggt
     1801 aaggggcccg ccccttacct gactgattat tgaatgatgc cgcaggccat tctcgcacca
     1861 ccaccgccca ggggctccgg atggtcatga tggttatcac cgccagcatg aagcatgaga
     1921 gaacgccctt taatctcttt taatgagttc agtctcgggg ccaggaccgg gtagttcgct
     1981 tttccgtcat gcgtcacgaa cagcgcaggg aggtcgccca ggtgtccatc cggagaccag
     2041 gggccaagat gtttgccggt gtttttcggg tcaaagtgac cgccagccga taatgctgcg
     2101 accggttttc cgtctttcag tgccggggcg caatttcctt tttcgtgcac atgaaaacca
     2161 tgaatgcctt cagacagaga gtgaagggct ggtgtgaaca gcagaccgta gggggtctcc
     2221 tgaatggtta tttttccaat gctgacttct tttccgtcag cactgacaag gttcattggg
     2281 acttcctgtt ctgctgcgta tccgcatgat gctgctgtca gcatggcaat ggcagcaatg
     2341 attttacatt tcataaaacc ctcattaatt ccgttaacag actgagcttg ctggttacag
     2401 ggtaacaaac agcgttctga tgatatcgcg caatagctgt gcaatatcct atcactgcga
     2461 ttaataatac caattgagag gaacattatg ggtaaaggtg gcggcagggc gcacacgccg
     2521 gttgaggcaa aggacaatct taagtccacg cagatgatga gcgtgattga tgccattggt
     2581 gaagggccga ttgaaggtcc ggtgaagggg ttgcagagta tcctggtgaa caaaactccg
     2641 ctgacggaca cggacggtaa tcctgtgata catggtgtga cagcggtctg gcgcgccggg
     2701 gagcaggagc agacaccacc tgaaggcttt gagtcttccg gagctgaaac cgcactgggc
     2761 gtggaagtga cgaaggcaaa gccggtgacg cgcaccatta cgtccgcgaa tattgaccgc
     2821 ctgcgggtca ccttcggggt gcagtcactg ttggagacca cctcaaaggg cgaccgtaat
     2881 ccctcttctg tccgactgct gattcagttg cagcgtaacg gtaactgggt gacggaaaa
//
LOCUS       DAZKIV010000288         2930 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZKIV010000288 DAZKIV010000000
VERSION     DAZKIV010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.290"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            215..940
                     /locus_tag="ACHOT6_004800"
     CDS             215..940
                     /locus_tag="ACHOT6_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949858.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            898..1575
                     /gene="bglJ"
                     /locus_tag="ACHOT6_004801"
     CDS             898..1575
                     /gene="bglJ"
                     /locus_tag="ACHOT6_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFP9949859.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1613..2401)
                     /gene="fhuF"
                     /locus_tag="ACHOT6_004802"
     CDS             complement(1613..2401)
                     /gene="fhuF"
                     /locus_tag="ACHOT6_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFP9949860.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2542..2778
                     /locus_tag="ACHOT6_004803"
     CDS             2542..2778
                     /locus_tag="ACHOT6_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFP9949861.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            complement(2817..2903)
                     /locus_tag="ACHOT6_004804"
     tRNA            complement(2817..2903)
                     /locus_tag="ACHOT6_004804"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2867..2869),aa:Leu,seq:cag)
BASE COUNT          776 a          637 c          727 g          790 t
ORIGIN      
        1 ttttaaatgt ctccaagcta tttcaccagg ttatcatttg atgctaacct tctgacacat
       61 gcagtggagt tgttgtgcag caggagtatg ctgatatgaa agtgaatgct aaggataatt
      121 tattcgctta atctattaat ttgctggaaa catttaagga atgaatatcc tgaataaacc
      181 atcataattt ttagacagtg gatgtggagg aaatatgttg ccaggatgct gcaaaaatgg
      241 aattgttatc agtaaaatac ctgttatgca agcagggcta aaagaggtca tgaggactca
      301 cttccctgaa tatgaaataa tatccagcgc ctctgcggag gaccttacct tattacaatt
      361 acgtcgttcc ggattagtca ttgctgattt agccggtgaa agtgaagatc cacgttctgt
      421 ttgtgaacat tattattctt taatctcaca atatcgggaa attcactggg ttttcatggt
      481 gtcacgctcc tggtattccc aggcagtaga actgctcatg tgccctacgg cgacgttatt
      541 gtctgatgtt gaacccattg agaatctggt caagaccgta cgttccggca atacgcacgc
      601 agagcgtatc agcgccatgc tgacctctcc ggcaatgact gaaactcatg attttagcta
      661 tcgctccgtc attctcactc tttcagagcg caaggtactg cggctattag gtaaaggatg
      721 gggcatcaac cagatagctt cattgcttaa gaaaagtaat aaaactatca gcgcccaaaa
      781 aaacagtgcg atgcgtcgac tggcaattca cagcaacgct gaaatgtatg catggataaa
      841 tagcgcgcag ggtgcaagag aacttaactt gccttctgtt tatggagatg ccgcagaatg
      901 gaacacagcc gaattaagaa gagaaatgtc gcactcatag aaaaatgcgt catgagtagt
      961 atcggtattg agagtttatt cagaaagttt gcgggtagcc cttataagct ccatacctat
     1021 accagtcagg agtcatttca ggatgccatg tcgcggatct cgtttgcggc ggtcattttt
     1081 tctttttctg ccatgagaag tgagcgcaga gagggattat cttgcctgac tgaactggcg
     1141 attaagtttc cgcgtacccg gcgtttagtt attgcggatg atgatattga agctcggctg
     1201 attggttcat tgtcgccatc accgctggac ggtgtattaa gtaaagcgtc aacgctggag
     1261 atttttcatc aggaactctt tttgtcatta aatggtgtac gtcaggcgac cgaccgactg
     1321 aacaatcagt ggtacattaa ccaaagccgg acgctaagcc cgacggagag agaaatattg
     1381 cgctttatgt cgcgtggcta ctcaatgaca caaattgccg agcagcttaa acgtaatatc
     1441 aaaacgatcc gtgcacataa atttaatgtg atgtcgaaac tgggcgtcag ttctgatgca
     1501 gggttgttgg aggccgcaga tattctgtta tgtatgcggc attgcgaaac aagtaatgtg
     1561 ttgcatccct attaatccgc atgatgccgg gtttacttcc ccggcagtgc tttcatttca
     1621 gcgtacaatc gccacattgc tgcacatccg gtaagcgata acgctggcag caagtgcggc
     1681 gcaccagcag gccatcgcgc agtaccacgg tacgccagag tggattatct tcaccgttcg
     1741 tgagcgtttt ctcaaaaaag agggcatggc gcagcgattc aaccgtcgcc tcgccgagca
     1801 gttgcttcat ctcagtaaga taccagttga tcaaataacc ggtattactc cagataagtt
     1861 tgccgttgat ctctccggtc gcttctagtg cctgcacaac cggaaccagc gcctggctga
     1921 ttaacctttc cattcgctgc tgcggcgaat gtggtgttgc gtttttatct tcacacacat
     1981 cgacccagaa acaggcggca cggccggttt cgtgaaactc agcatggaaa tgttccggcg
     2041 acacatctaa tgccttttcc tgcgtcagta gcgccagcat taatggtggc accatcaggc
     2101 cgatatacca ttgtgcccat agtgagatca gcggtttgtt ctcgcggatc atcgttggtt
     2161 ggttgcgata gatatgatcg gaatagaccg cgagtagaga acttagcgca ttcggtgatg
     2221 accattgcgc cagcgtcatg gcgttaagcg gagcgggttc atccaggcgg ataaactcca
     2281 gcaaatgttc acgatgttcg gcgatcgtcg cacgtacggc ttgtgcaagc ccagcatcct
     2341 gtggctggag atgcgttcgc cagataacat cttcatagag cggtgcggaa cgataggcca
     2401 taatcgggat agtaatctaa atgataatga ttgctaatcc tagcgatagg tttacccgat
     2461 agcaagggat ttctctggct tgcaaatgat aaaaattatc atatgatatt ggttatcatt
     2521 atcaatgaaa gagatgaaac catgttgcaa cgtacgctgg gcagtggctg gggagtgttg
     2581 ctgccaggat tgctgattgc agggctgatg tatgcggatt tatcaccgga tcagtggcgg
     2641 attgtcattc tgatgggatt agtattgacg tcggtgatgc tgtatcacaa acagttacgg
     2701 cattacgttt tgctaccatc gtgcctggcg cttattgctg ccatcatgct gatgataatg
     2761 aatttgaatc agggatgaaa aatcaaggaa gaaacaagaa aggaagtaaa gataattggt
     2821 gcgagggggg ggacttgaac ccccacgtcc gtaaggacac taacacctga agctagcgcg
     2881 tctaccaatt ccgccacctt cgcacagtca tcttactttt tttgatatcg
//
LOCUS       DAZKIV010000289         2884 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZKIV010000289 DAZKIV010000000
VERSION     DAZKIV010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.291"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(148..750)
                     /locus_tag="ACHOT6_004805"
     CDS             complement(148..750)
                     /locus_tag="ACHOT6_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949862.1"
                     /translation="MSDDSNLNNHGKQDTNISESRRLKVVGGSDFEAEFDNSPTKVQN
                     NYIKPPQTEEEVGTISREELDARLAANKAEMESIASSIRADMALSRESVNVQFASLNA
                     AISSLSSKIDGKMDSAAGDLKAINGRFEGIQGQITGVNTAISGVQSGISIRLAIFSVI
                     IAVIVAIPGLVSALKSDHAPLQAPPQNTQPTDNKKITPQH"
     gene            complement(747..1181)
                     /locus_tag="ACHOT6_004806"
     CDS             complement(747..1181)
                     /locus_tag="ACHOT6_004806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001207140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949863.1"
                     /translation="MALNLERISFITPFDSSEEPNQPTLNFTCNEFPARLSIDFRVGM
                     IGLKPNSRYNLGIMVIPAHLIIKKGEEIQFPDGSSESVSLFIDTKDSHFETGVGGQVI
                     VTLKEIRVPAKGLYSVIGRLQDNEDPKNELHKNESFFTVELL"
     gene            complement(1232..1945)
                     /locus_tag="ACHOT6_004807"
     CDS             complement(1232..1945)
                     /locus_tag="ACHOT6_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFP9949864.1"
                     /translation="MSAKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMG
                     QSGVGALFNGINALNAYNAALLAKILNVSVEEFSPSIAREIYEMYEAVSMQPSLRSEY
                     EYPVFSHVQAGMFSPELRTFTKSDAEKWVSTTKKASGSAFWLEVEGNSMTAPTGYKPS
                     FPDGMLILVDPEQTVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNE
                     SCSVVGKVIASQWPEETFG"
     gene            2046..2246
                     /locus_tag="ACHOT6_004808"
     CDS             2046..2246
                     /locus_tag="ACHOT6_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9949865.1"
                     /translation="MEQRITLKDYAMRFGQTKTAKDLGVYQSAINKAIHAGRKIFLTI
                     NADGSVYAEEVKPFPSNKKTTA"
     gene            2365..2658
                     /locus_tag="ACHOT6_004809"
     CDS             2365..2658
                     /locus_tag="ACHOT6_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFP9949866.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGGVDDDMARLARQVAAILTNKKRPAATERSEQIQMEF"
     gene            2691..>2884
                     /locus_tag="ACHOT6_004810"
     CDS             2691..>2884
                     /locus_tag="ACHOT6_004810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFP9949867.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNMLLEAYSGAD
                     LTKRQFKVLLAILRKTYGWN"
BASE COUNT          800 a          636 c          619 g          829 t
ORIGIN      
        1 tgagtgtatt gcctcggcga tgtaaggaga gatcagaatt gcgttgttta gtgagttgta
       61 tctatttatt ttccaataaa tacaattggt tatgtgtttt taggtggggc gatcgtgagg
      121 caaagaaaaa ccggcgctga ggccgggtta atgctgggga gtgatttttt tgttgtctgt
      181 tggctgggta ttttgtggcg gcgcttgcaa aggtgcgtgg tctgacttta aagctgatac
      241 cagcccggga atcgcaacta ttacagcgat aatgacacta aaaatggcta atcgtataga
      301 aatacccgac tgaacaccac taattgcggt attaacccct gttatttgtc cttgaattcc
      361 ttcgaatctc ccattaattg cttttaaatc gccagcagcg ctatccattt ttccatcgat
      421 cttggacgat agagagctta tggccgcatt aagtgatgca aattggacat taacgctttc
      481 acgagataga gccatgtcag ccctaatgga agaagcaata gactccatct ctgctttatt
      541 agcagctaag cgagcatcaa gttcctctct gctgatcgtt cccacttctt cctccgtttg
      601 tggcggtttt atgtaattat tttgcacctt ggtgggagaa ttatcaaatt cagcctcaaa
      661 atcggaaccg ccaacaacct tcaacctccg gctttcagat atgttggtgt cttgctttcc
      721 atgattattt aaattgctgt cgtcactcat aatagctcta cggtgaaaaa tgattcattt
      781 ttatgaagtt catttttagg gtcttcatta tcttgcaatc tccctataac actatacagc
      841 cctttagctg ggaccctaat ttctttcaat gttactatta cctgtccgcc aacccctgtt
      901 tcaaaatggc tatctttcgt atcgatgaaa agtgaaactg attccgaaga gccgtcaggg
      961 aattgaattt cctcaccttt ttttataatt aggtgcgcgg ggattaccat aatacccaaa
     1021 ttatatcttg aatttggttt caacccgatc ataccaaccc tgaaatcaat tgatagccgc
     1081 gcaggaaatt cattgcatgt aaaattaagc gtcggttggt tgggttcttc gctgctatca
     1141 aatggggtta taaaagatat tctttctaaa ttaagtgcca taaattctcc tgcatcgctg
     1201 tatgctctac gagcatctcg gcgacttatc atcacccaaa cgtctcttca ggccactgac
     1261 tagcgataac tttccccaca acggaacaac tctcattgca tgggatcatt gggtattgtg
     1321 ggtttagtgg ttgtaggaac acctgaccgc tatccctgat cagtttcttg aaggtaaact
     1381 cgtcaccacc aagtctggct atgcagaaat caccaggctc aacagtctgc tcagggtcaa
     1441 caagaattaa catcccgtca ggaaagcttg gcttgtagcc tgttggtgcg gtcatggaat
     1501 taccttcaac ctcaagccag aatgcagagc cactggcttt tttagttgtg cttacccatt
     1561 tctccgcatc acttttggta aaggttctaa gctcaggcga gaacatcccg gcctgaacat
     1621 gagaaaaaac agggtactca tactcacttc taagtgacgg ctgcatacta accgcttcat
     1681 acatctcgta gatttctctg gcgattgaag ggctaaattc ttcaacgcta acgttgagaa
     1741 tttttgcaag caatgcggcg ttataagcat ttaatgcatt gataccatta aataaagcac
     1801 caacgcctga ctgccccatc cccatcttgt ctgcgacaga ttcctgagat aagccaagtt
     1861 catttttctt tttttcataa atagctttaa ggcgacgtgc gtcctcaagc tgctcttgtg
     1921 ttaacggttt cttttttgcg ctcatacatt aaatctatca ccgcaaggga taaatatcta
     1981 acaccgtgcg tgttgactgt tttacctcta gcggtgataa taattgcatg tactaaggag
     2041 gttctatgga acaacgcata accctgaaag attatgcaat gcgctttggg caaaccaaga
     2101 cggctaaaga tctcggcgta tatcaaagcg cgattaacaa ggccattcat gcaggccgaa
     2161 agattttttt aactataaac gcagatggaa gcgtttatgc ggaagaggta aagcccttcc
     2221 cgagtaacaa aaaaacaaca gcataaataa caccgctctt acacatcaca gccctgaaaa
     2281 agggcatcca attaaaccac acctatggtg tatgcattta tttgcataca ttcaatcaat
     2341 tgttatctaa ggaaatactt acatatggtt cgtgcaaaca aacgcaacga ggcactacga
     2401 atcgagagtg cgttgcttaa caaaatcgca atgcttggaa ctgagaagac agcggaagct
     2461 gtgggagttg ataagtcgca gatcagcagg tggaagagag actggattcc caagttctca
     2521 atgctgcttg ctgttcttga atgggggggc gttgacgacg acatggctcg attggcgcga
     2581 caagttgctg cgattctcac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa
     2641 atccagatgg agttctgagg tcattactgg atctatcaac aggagtcatt atgacaaata
     2701 cagcaaaaat actcaacttc ggcagaggta actttgccgg acaggagcgt aatgtggcag
     2761 atctcgatga tggttacgcc agactatcaa atatgctgct tgaggcttat tcgggcgcag
     2821 atctgaccaa gcgacagttt aaagtgctgc ttgccattct gcgtaaaacc tatgggtgga
     2881 ataa
//
LOCUS       DAZKIV010000290         2883 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZKIV010000290 DAZKIV010000000
VERSION     DAZKIV010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.292"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1153)
                     /locus_tag="ACHOT6_004811"
     CDS             complement(join(<1..862,862..1153))
                     /locus_tag="ACHOT6_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9949868.1"
                     /translation="MTKNTRFSPEVRQRAARMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTGGGDGGLTTAERQRLKELERENRELRRSNDILRQASAYFGEGGV
                     RPPLEKMMPLLDKLREQYGVGPLCSELHIAPSTYYHCQQQRHHPDKRSARAQRDDWLK
                     KEIQRVYDENHKVYGVRKVWRQLLREGIRVARCTVARLMAVMGLAGVLRGKKVRTTIS
                     RKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVAFIIDVFAGYIVGWRVSSS
                     METTFVLDALEQVLWARRPSGTVHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGDSYD
                     NAMAESINGLYKAEVIHRKSWKNRAEVELATLTWVDWYNNRRLLERLGHTP"
     misc_feature    complement(756..872)
                     /locus_tag="ACHOT6_004811"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01497"
     gene            complement(1242..2117)
                     /locus_tag="ACHOT6_004812"
     CDS             complement(1242..2117)
                     /locus_tag="ACHOT6_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
                     /protein_id="HFP9949869.1"
                     /translation="MFSIRSLLPISASVSVPAKQSHPIPTTLAGRTIEKAQEKEGLLV
                     FLGMKSVNEYTLNILGQNVSRVTTGKKPYDLLFLNNATKQDFDKRKMEFTYPGANKSH
                     LQSSNSDVVAAAAISIAATEIKTILPDDLTPGKYNKIYLSGHGSAGLPLLKCGDEFLS
                     PADIVDRIVQYNLHEIDDIRLTSCNSANIIKNKDFSPDEIDKSSNINNGWLARTLFGQ
                     KKSLAEHVYAEFESRGINVSISGYHGTGVFYVPEHGKPTTHLRSTTVPATPEYTVRRS
                     DYRATFGRTQPIDID"
     gene            complement(2390..2822)
                     /locus_tag="ACHOT6_004813"
                     /pseudo
     CDS             complement(2390..2822)
                     /locus_tag="ACHOT6_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309841.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein NleH"
BASE COUNT          740 a          702 c          569 g          872 t
ORIGIN      
        1 ggagtatggc ccagcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggccagtt ccacttctgc acggtttttc cagctcttac ggtgtattac ctccgctttg
      121 taaagaccat tgatgctctc cgccatcgcg ttgtcatacg agtcgcctgt acttcctgtt
      181 gatgccagta atccggcttc cttaagccgc tgtgtgtagg ccagcgatac atactgagaa
      241 cctttatcac tgtgatggac cgtgccggac ggtcgacggg cccataacac ctgctccagt
      301 gcatccagca cgaatgtcgt ctccatggac gatgagaccc gccaccccac aatgtatccg
      361 gcaaacacat caatgatgaa cgccacatag acgaagccct gccatgtgct gacgtaagta
      421 aaatcagcca cccacagctg gtcaggtcgt tctgccacga actgacggtt tacgcggtcg
      481 cctgcggcaa cggctttccg gctgatggtc gtacggacct ttttaccccg gagaacaccg
      541 gcaagtccca taaccgccat gagacgtgcc acagtgcatc tggccactct gataccttcc
      601 cgtaacaact gacgccagac tttacgcaca ccgtatacct tgtgattttc atcgtatacg
      661 cgctgtatct ctttcttcag ccagtcatcg cgctgcgcac gggcactgcg tttatccgga
      721 tgatgtcgct gttgctgaca gtggtaatac gttgacgggg caatatgcag ttcgctgcat
      781 agcggtccga ccccgtactg ctcacgcagc ttatccagca gtggcatcat tttttccaga
      841 ggcggtcgaa ctccgccttc gcaaaataag cggaagcctg gcgaaggata tcgttactgc
      901 ggcgcagttc acgattttca cgctccagct ctttcagacg ctgacgttca gcggtggtga
      961 gccctccatc accgcccccg gtatcccgct catgctggcg aacccagaca cgcagagtct
     1021 ccggcgtaca gccaatcttt ggagcaatgg aacaaattgt cgcccattgt gagtcatatt
     1081 cgccctgact ttccagaacc atacgggctg cccgttgacg gacttcaggg gaaaaacgag
     1141 tatttttagt catcctgttt acctctttct caggaagttt agtctccagg attcccgggg
     1201 cggttcagtt tcctaaccat caagtgtata tgctgactaa attagtcaat atctatgggt
     1261 tgagttctac caaaagtggc tctgtagtcg cttcttctta cagtatattc aggtgttgca
     1321 ggaacagttg tggagcgtag atgcgttgtt ggtttaccat gctctggtac ataaaaaacg
     1381 ccagtgccat ggtaacctga tatagaaacg ttaattccac gactttcaaa ctcggcatag
     1441 acgtgttctg ctaaagactt cttttgacca aataatgtcc tggccaacca gccgttatta
     1501 atatttgagg atttatctat ttcatcagga gagaagtctt tgttttttat tatgttggct
     1561 gagttacagg atgttaatct gatatcatct atttcatgaa gattatattg aacaatgcgg
     1621 tcgacaatat ctgccggtga taaaaattca tctccgcact taagaagagg tagaccagca
     1681 gaaccatgcc cagatagata aattttgttg tattttcctg gtgttaaatc atctggcagg
     1741 atggttttta tctctgtcgc tgcaatactt atagctgcag cagcaacaac atcgctatta
     1801 cttgattgta gatggctttt atttgctcca ggatatgtga attccatttt ccttttatca
     1861 aaatcttgtt ttgtagcatt attcaggaat aataaatcat acggtttttt ccccgttgtg
     1921 actcttgaaa cattttggcc aagaatatta agagtatatt cattaacgga tttcattcct
     1981 aaaaaaacaa gtaatccttc tttctcttgc gctttttcga ttgttctccc tgctagagtt
     2041 gttgggatgg ggtgagattg ttttgctgga actgatacgc tggcgctaat aggtagtagg
     2101 gatcttatgc taaacataca acctctttct taatttgcga acctgtttag gattctatgt
     2161 aaatgtcttt tctcagaagt gaccaccccg gcggtcaccg aactcgataa taaagcggat
     2221 cattgccatt ttccagtcct cagcggcatt gtctatttcc gtgatccgcc aagaattgcc
     2281 agctatacta ctttttacta actcatctat cggatatatt ttgcatatta aatataccct
     2341 catcaaaaac cccatccatg taacaaatcc actacactaa ataaaattac taaattctac
     2401 ttaatactac actaataaga tcttgctttc ctccatgata agatagcatg acttgatgct
     2461 cactccatga aatatcagaa acattataag attatatacc tattggattg aattcatttc
     2521 tcatacgatc atataaaaca ttttttctgt tgtgtcaaca aaaagaattc ctttttcctc
     2581 cagtctgtca aacatatcgt aaatagcctg ctcggcttgt gctggtaatg atggaatatc
     2641 caaaagagat tccccatttt tttattcatt ctaatgccaa taacatctcc attatcatta
     2701 tatattttct ctgcactcct agcaccatca tactggttga aacaacgaac ttcgcttgtc
     2761 acctcttcat ggctttgaga tatagtaaac atcttcaaca cttttgttgt atcttccata
     2821 tcttatatat gacacaaaaa gcgaccacta aagtcgcttt ttcttatggt aacaggcaat
     2881 aac
//
LOCUS       DAZKIV010000291         2864 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZKIV010000291 DAZKIV010000000
VERSION     DAZKIV010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.293"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            291..1457
                     /gene="nhaA"
                     /locus_tag="ACHOT6_004814"
     CDS             291..1457
                     /gene="nhaA"
                     /locus_tag="ACHOT6_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFP9949870.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1523..2422
                     /gene="nhaR"
                     /locus_tag="ACHOT6_004815"
     CDS             1523..2422
                     /gene="nhaR"
                     /locus_tag="ACHOT6_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFP9949871.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2461..>2864)
                     /locus_tag="ACHOT6_004816"
     CDS             complement(2461..>2864)
                     /locus_tag="ACHOT6_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HFP9949872.1"
                     /translation="SIDTGRFVLSRINNENYQFHHIRFDCIQGEQGELVFEPSDVEYY
                     FEPVTIQESGTSILKNELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSN
                     QIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT          683 a          629 c          714 g          838 t
ORIGIN      
        1 ggattatttt tgaaatattg tcctaacaaa taacgccgac tgacaagaaa ttaattatta
       61 cttttcctaa ttaatccctc agaaatcctc accttaagct atgattatct gggcttaggg
      121 tcactcgtga gcgcttacag ccgtcaaaaa ctcatctcac cgctgatggc gcaaattctt
      181 caatggatcg taaaaaacga gttattccta cactataatc tgattttaac gatgatttgt
      241 gcggggtaaa atagtaaaaa cgatctattc acctgaaaga gaaataaaaa gtgaaacatc
      301 tgcatcgatt ctttagcagt gatgcctcgg gaggcattat tcttatcatt gccgctatcc
      361 tggcgatgat tatggccaat agcggcgcaa ccagtggatg gtatcacgac tttctggaga
      421 cgccggttca gctccgggtt ggttcactcg aaatcaacaa aaacatgctg ttatggataa
      481 atgacgcgct gatggcggta tttttcctgt tagtcggtct ggaagttaaa cgtgaactga
      541 tgcaaggatc gctagccagc ttacgccagg ccgcatttcc agttatcgcc gctattggtg
      601 ggatgattgt tccggcatta ctctatctgg cctttaacta tgccgatccg attacccgcg
      661 aagggtgggc gatcccggcg gctactgaca ttgcctttgc acttggtgtg ctggcgctgt
      721 tgggaagtcg tgttccgtta gcgctgaaga tctttttgat ggctctggct attatcgacg
      781 atcttggggc catcattatc atcgcattgt tctacactaa tgacttatcg atggcctctc
      841 ttggcgtcgc ggctgtagca attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc
      901 gcacgggcgt ctatattctg gttggcgtag tgttgtggac tgcggtgttg aaatcggggg
      961 ttcacgcaac cctggcgggg gtaattgtcg gcttctttat tcctttgaaa gagaagcatg
     1021 ggcgttctcc agcgaagcga ctggagcatg tgttacaccc gtgggtggcg tatctgattt
     1081 tgccgctgtt tgcatttgct aatgctggcg tttcactgca aggtgtcacg ctggatggct
     1141 tgacctccat tctgccattg gggatcatag ctggcttgct gattggcaaa ccgctgggga
     1201 ttagtctgtt ctgctggttg gcgctgcgtt tgaaactggc gcatctgcct gagggaacga
     1261 cttatcagca aattatggcg gtggggatcc tgtgcggtat cggttttact atgtctatct
     1321 ttattgccag cttggccttt ggtagcgtag atccagaact gattaactgg gcgaaactcg
     1381 gtatcctggt cggttctatc tcttcggcgg taattggata cagctggtta cgcgttcgtt
     1441 tgcgtccatc agtttgacag gacggtttac cggggagcca taaacggctc ccttttcatt
     1501 gttatcaggg agagaaatga gcatgtctca tatcaattac aaccacttgt attacttctg
     1561 gcatgtctat aaagaaggtt ccgtggttgg cgcagcggag gcgctttatt taactccaca
     1621 aaccattacc ggacagattc gagcgctgga agagcgcctg caaggcaaat tatttaaacg
     1681 caagggacgt ggtctcgaac ccagcgagct gggagaactg gtctatcgct atgccgataa
     1741 aatgttcacc ttaagccagg aaatgctgga tattgtgaac tatcgcaaag aatccaattt
     1801 attgtttgac gttggtgtgg ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa
     1861 cgccgcagtg gtagaaggcg agcccattca tcttcgctgc ttcgaatcca cccacgaaat
     1921 gctgctggag caattaagtc agcataaact ggatatgatc atttctgact gtccgataga
     1981 ctctacgcag caggaaggcc tgttctccgt gagaattggc gaatgtggcg tgagtttctg
     2041 gtgtacaaat ccaccaccag aaaaaccgtt cccggcttgt ctggaagaac ggcgactttt
     2101 gattcctggg cgacgttcaa tgttagggcg caaattgctt aactggttta actcccaggg
     2161 attaaacgta gaaatcctcg gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc
     2221 gatgcacaat gcaatcttcg ttgccccaac gctttatgca tatgactttt atgccgataa
     2281 aactgtcgta gaaattggtc gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc
     2341 tgagcggatg attcagcacc cggcggtaca gcgaatctgc aatacggatt attctgcgct
     2401 ttttagtcca gcggcgcgtt aatcggcacc tccccccaaa gttaaggtgg gggagataga
     2461 ttagttgtac ataaccacga ttttgactcg gctcattatt tgcccgcttg agacattatt
     2521 tccatatgta cgcgggcgaa taaatagagg aatctgatta cttccttcat ggggatgctg
     2581 aaaagagtag taattgcttt taccatactg gatttcattc gtaccatcat tggagagttg
     2641 aaagcctatt tgacccgcac cgtcctcact gttttctaac tcatttttta atatgctggt
     2701 tcctgactcc tgtatcgtca ctggttcaaa gtaatattca acatcactgg gttcaaaaac
     2761 taattcacct tgctcgccct ggatgcagtc aaatcttata tgatgaaact gataattttc
     2821 attattgata cgagacagta caaatcgtcc agtgtcgatg gatt
//
LOCUS       DAZKIV010000292         2860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZKIV010000292 DAZKIV010000000
VERSION     DAZKIV010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.294"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..1332
                     /gene="bluF"
                     /locus_tag="ACHOT6_004817"
     CDS             121..1332
                     /gene="bluF"
                     /locus_tag="ACHOT6_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFP9949873.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
     gene            1537..2268
                     /gene="bluR"
                     /locus_tag="ACHOT6_004818"
     CDS             1537..2268
                     /gene="bluR"
                     /locus_tag="ACHOT6_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFP9949874.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYTAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
     gene            2489..>2860
                     /locus_tag="ACHOT6_004819"
     CDS             2489..>2860
                     /locus_tag="ACHOT6_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFP9949875.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYI"
BASE COUNT          830 a          609 c          614 g          807 t
ORIGIN      
        1 aactttttcc attttttatc aatgtaaaga aacagttatg ttttggtatt aaaagaaaga
       61 cacaagaaga tatgtctgtt accgtcttac tctcgcctca cccattaccc tggattgtta
      121 atgcttacca cccttattta tcgtagccat atacgtgacg acgaacctgt caaaaaaatc
      181 gaagaaatgg tttcgatagc aaatcgcagg aacatgcagt ctgacgtaac agggatctta
      241 ctgtttaatg gttctcattt tttccagctt ctggaaggtc cggaagaaca ggttaaaatg
      301 atatatcggg ctatatgcca ggatccacgg cactataata ttgttgagct gctgtgcgat
      361 tacgcgcctg ctcgccgttt tggcaaagcg ggaatggaat tatttgattt gcgcctgcac
      421 gagcgagatg acgttttaca ggccgtattc gacaaaggca catcaaaatt tcagctaact
      481 tatgatgaca gagcgctaca attttttcgt acttttgtcc ttgcaaccga acaatcaacc
      541 tatttcgaga tccctgccga agactcctgg ctttttatcg ctgacggatc tgataaagaa
      601 cttgattcct gtaccctttc accaactata aacgaccact ttgcctttca tcctattgtc
      661 gatcccttat cgcggcggat aatcgctttt gaagccattg tgcaaaaaaa tgaagatagc
      721 ccatcagcca tagcggttgg gcagcgtaaa gacggggaaa tctacacagc ggatctcaaa
      781 agtaaggcgc ttgcattcgc gatggcacac gcacttgagc tcggtgataa aatgatttca
      841 atcaatctat tacctatgac ccttgttaac gaacctgacg cagtctcttt tttacttgat
      901 gaaataaagg ccaatgctct ggtgcctgaa caaatcatcg ttgaatttac tgaaagtgaa
      961 gttatatctc ggtttgatga gtttgcagaa gcgattaaat cgcttaaggc tgccggtatc
     1021 agtgtagcaa ttgatcattt tggcgcaggt tttgccggtt tgttactcct gtcacgcttc
     1081 cagcctgaca gaattaaaat cagtcaggaa ttgattacca atgttcataa aagcgggcca
     1141 cggcaggcaa ttattcaagc gatcataaaa tgctgtacat cacttgaaat tcaagtcagt
     1201 gctatgggcg tggcaacacc agaagagtgg atgtggcttg aatccgcagg aattgagatg
     1261 tttcagggag atctgtttgc taaagctaaa ttgaatggta tcccttccat agcgtggcca
     1321 gagaaaaaat aattttcagc acattctttc acatgatttc agtaaatcat cgtgaaaatg
     1381 aatacgttat gtttaaattt aatccacatt aaatataatg tttcatccaa tttagctgaa
     1441 atcttattta tactttgtac aattttagcg tatattgtac aaagtattat tgggtcgtgt
     1501 acaggcggca gagatttatg accgcaagga ggaattgtgg cttattacag cattggtgat
     1561 gttgctgaac gttgcgggat taatcctgtc actctccggg cctggcaacg ccgctacggt
     1621 ttgttaaaac cacagcgcag tgaaggcgga caccgactct ttgatgaaga agacatacaa
     1681 cgtatcgaag agatcaagcg ttggataagt aatggcgtcc ctgtaggcaa agttaaagca
     1741 ttactggaaa ccaccagcca ggatacggaa gatgactgga gccgcttgca agaagagatg
     1801 atgtcaattc ttcgtatggc taatcctgcc aaactacgcg cgagaattat ttcactgggt
     1861 cgagagtacc cagtagatca attgattaat catgtttacc ttcctgttcg ccagcgtctc
     1921 gtgcttgatc acaacacctc ccgcattatg agcagtatgt ttgacggcgc attaattgaa
     1981 tacacagcag cctcgctttt cgaaatgcgc cgtaagcccg gtaaagaagc cattctgatg
     2041 gcgtggaatg ttgaagagag agcacgattg tggctggaag cctggcgttt atcattgtca
     2101 ggatggcaca tttctgttct tgctgatccc attgaatcgc cacgcccgga actgttcccg
     2161 acgcaaacat tgattgtctg gacaggcatg gcaccaacga gaaggcaaaa cgaactttta
     2221 caacattggg gtgagcaagg ttataaagtc atctttcatg ccccctaatt tcacctttat
     2281 cccgcccctt agacagaatg ttataatctg aacccacaac agcgtttgta ccggagctgg
     2341 tccgttgcaa aatgctcacc gggagaattc agatattccc tctatcacac tctgtacata
     2401 tcacagccaa aggacagacc agattgaagt ggtaaaaccg atcgtgcaca ctacgcagtc
     2461 tcacaactaa caagaggtta ttatcctgat ggatcaggtc gttgtttttc aaaagatgtt
     2521 tgagcaagtg agaaaagagc aaaacttctc ctggttttat tcagaattaa aacatcaccg
     2581 tattgcacat tacatttatt atctggctac ggataacatc agaattatta ctcacgatga
     2641 cacggttttg ttattaagag gaaccaggaa cctgttaaaa gttagtacga ccaagaaccc
     2701 tgctaaaata aaagaggccg cattgcttca tatttgcgga aaatctacat ttcgggaata
     2761 ctgttcaaca ctagcaggcg cgggcgtttt ccgttgggtt actgatgtta atcataacaa
     2821 acgcagttac tacgccattg ataatacgct tttatatatt
//
LOCUS       DAZKIV010000293         2853 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZKIV010000293 DAZKIV010000000
VERSION     DAZKIV010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.295"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            436..624
                     /gene="dicB"
                     /locus_tag="ACHOT6_004820"
     CDS             436..624
                     /gene="dicB"
                     /locus_tag="ACHOT6_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9949876.1"
                     /translation="METLLPNVNTSEGCFDIGVLLSNREFTEDAINMRKYEPYLLNDN
                     SILSRIALLELGIFGERQ"
     gene            621..809
                     /locus_tag="ACHOT6_004821"
     CDS             621..809
                     /locus_tag="ACHOT6_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9949877.1"
                     /translation="MTSAFALMMTVFLITGESQNVITGIYASKESCLQARDEQKISGE
                     CLPVKKVSLYLNNETPAG"
     gene            902..>2853
                     /locus_tag="ACHOT6_004822"
     CDS             902..>2853
                     /locus_tag="ACHOT6_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFP9949878.1"
                     /translation="MSTDKEEFALYCEAKNDKVRKRLGIKGGFYWTTAKKLSVAISRC
                     ITAMDDNDYDEDDFKKPVRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMVFIAPS
                     PSVQEKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVAREEL
                     QALHIARAEELPAVTALAISHKTKLLDPLEIRDLHTLVRDTDKVFPNPGNSDLGLITA
                     FFEAYLDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNKTDRNPNLVHTFDTLD
                     VEIAAATLPMDFNIYEIPGSVYRRAKEIVLKRESPFKEWSAALRATPGILDYSRAAIF
                     ALIRSAHPEFYHYPGRLQGYINAYLTETDHENPSKETLTAARHTPEKDILEEVNRELS
                     AKQETEEEENDEEKPQPSCAMADEQATAETVEPDATEHHQDTQPLDAQSQVNSVDAKY
                     QKLRAELHEARKNIPPQNPVDADKLLAASRGEFVEGISDPNDPKWIKGIQTRDSVYQN
                     QQETEQNDQKAEQNSPNALQNEPETKQPEPVAQQEVEKVCTACGQTGGGNCPDCGAVM
                     GDATYQETFDDKNLVEVQEDDSEKMEGAEHPHKENAGSAQDHASDSETGETADPLITV
                     NGHHVITSTSRTCDHLMIDLETMGKNP"
BASE COUNT          834 a          685 c          698 g          636 t
ORIGIN      
        1 gcataacgcg aaagcgtacg gcgaagctct ttcccttaga aggcttgtcg ttagatttct
       61 tcgaacgtgc gcttccggtt gtggcactcc gcgaaatggc gcggcggtaa gtatggcggg
      121 gttattcctt ccccattgag gacaccgggt tgtcaggttg accatacgct taagtgacaa
      181 ccccgctgca acgccctctg ttatcaattt tctggtgacg tttggcggta tcagttttac
      241 tccgtggctg ctctgccgcc ctttttaaag tgaattttgt gatgcggtga atgcggctaa
      301 gcgcacgcgg aacagttaaa accaaaaaca gtgttatggg tggattctct gtatccggcg
      361 ttaattgtta actggttaac gtcacctgga ggcaccaggc actgcatcac aaaattcatt
      421 gttgaggacg cgataatgga aacgttatta ccaaacgtta atacgtctga aggttgtttt
      481 gatattggtg ttctgctcag taaccgggag tttactgaag atgccattaa catgaggaaa
      541 tatgagcctt atctgctcaa tgataattcc atactttccc gaattgctct tcttgaactt
      601 ggtattttcg gagaacgtca atgacttcag catttgcact gatgatgacg gtttttctta
      661 taacgggtga atcacagaat gtgattaccg gaatttatgc cagtaaagaa tcctgcctcc
      721 aggcaagaga cgagcaaaaa atttctggtg aatgcctccc ggtaaaaaaa gtatcgctgt
      781 acctgaataa cgaaacaccg gctggataac cctccagcca tattaacacc ataccaacgg
      841 attaaaaatg ccagcaatgg cagggatttg ttcaccctta aatctgtaat gaggtttatc
      901 aatgagcact gataaagaag aatttgcgct atattgcgaa gcaaaaaatg acaaagtcag
      961 aaaacgtctg ggaattaaag gtggttttta ctggactaca gcaaaaaaat tatctgttgc
     1021 catctcccgc tgcattaccg caatggatga caacgattat gatgaagacg actttaaaaa
     1081 acccgttcgc gtccatttac ccgttgtgaa tgaccttcca cctgaaggcg tgtttgatac
     1141 cgaattctgc aaccgatacg aaaaaggcgg ggaagatggc attacaatgg tatttatcgc
     1201 gccctctccc tccgtgcagg agaaaccagc cagtactgac aataccaacg tcaacggcga
     1261 agacatgacg gagattgagg agaatatgct cctgccggtt tctggtcagg agctgcccat
     1321 tcgctggctt gcgcaacacg gcagcgaaaa accagtaacg cacgttgcac gggaagaact
     1381 tcaggcatta catatagcac gggcggaaga actgccggct gttactgccc tggccatttc
     1441 tcacaaaaca aagctgctcg acccgctgga gattcgcgac cttcacacac tggtacgcga
     1501 cactgacaaa gttttcccta atcccggtaa ttcagacctg ggactgataa ctgctttttt
     1561 cgaggcatac ctggacgctg actacactga tcggggtctg ctgacaaaag agtggatgaa
     1621 aggaaatcgt gtttcgcgta tcacccgtac ggcttccggt gcaaatgccg gtggcgggaa
     1681 caaaaccgat cgcaatccga atttagtaca caccttcgat acgctggatg tggagattgc
     1741 agcagccaca cttccgatgg attttaatat ttatgaaatt ccgggcagcg tttatcgtcg
     1801 cgcaaaagaa atcgtcctga aaagagaaag tccgttcaaa gaatggtccg cagcacttcg
     1861 cgcaaccccg ggtattctgg actattcccg cgccgctatt tttgcactta tccggagcgc
     1921 tcaccctgag ttttatcact acccgggacg ccttcagggg tatatcaacg cctacttaac
     1981 ggagactgat cacgagaacc ccagcaagga aactctcact gctgcacgac atacaccgga
     2041 aaaagatatc ctggaagaag ttaaccgcga actgtctgct aagcaggaaa cagaagaaga
     2101 agaaaatgat gaagaaaaac cgcaaccatc ttgcgcaatg gcagatgaac aggcaacggc
     2161 tgaaacagtg gaaccggatg caactgaaca tcatcaggac acgcagccgc tggatgctca
     2221 gtcacaggta aattctgttg atgcgaaata tcagaaactg cgggcagaac tccatgaagc
     2281 ccggaaaaac attccgcccc aaaatcctgt cgatgcagac aaattactgg ctgcctctcg
     2341 cggagaattt gttgaaggga ttagcgaccc gaatgatccg aaatggatta aggggatcca
     2401 gacccgcgat tctgtgtacc agaaccagca agaaacggaa cagaacgacc agaaagcgga
     2461 acaaaacagc ccaaatgcgt tacaaaacga gccagaaacg aaacagcctg aaccagtggc
     2521 gcaacaggaa gtggaaaaag tctgcaccgc ctgcggtcag accggcggcg gcaactgccc
     2581 tgattgtggc gcggtgatgg gcgacgcaac ataccaggaa acattcgatg acaagaacct
     2641 ggttgaagtt caggaagacg attcggagaa aatggaaggc gctgaacatc cacacaagga
     2701 gaatgctggc agcgctcagg accacgccag cgatagtgaa actggcgaga cggcagatcc
     2761 cttaattacg gtgaacggtc atcacgttat cacatccacc agcaggacgt gtgaccatct
     2821 aatgatcgac cttgaaacca tgggaaaaaa tcc
//
LOCUS       DAZKIV010000294         2796 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZKIV010000294 DAZKIV010000000
VERSION     DAZKIV010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.296"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            106..2385
                     /gene="ydeP"
                     /locus_tag="ACHOT6_004823"
     CDS             106..2385
                     /gene="ydeP"
                     /locus_tag="ACHOT6_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFP9949879.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDKHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2633..>2796
                     /gene="safA"
                     /locus_tag="ACHOT6_004824"
     CDS             2633..>2796
                     /gene="safA"
                     /locus_tag="ACHOT6_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFP9949880.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDL"
BASE COUNT          742 a          652 c          686 g          716 t
ORIGIN      
        1 agtcccctac tcttatatat ccatgttggc gataatccct ttgtatgtac tgtgcatcat
       61 cgctattaca aatcctaata attcatttcc acacaggata agtagatgaa gaaaaaaatt
      121 gaatcctacc agggtgctgc aggtggttgg ggtgctgtta aatccgtagc gaatgcagta
      181 cgtaagcaga tggatatacg ccaggatgtt attgccatgt ttgacatgaa taagccagag
      241 ggctttgact gtccgggttg tgcatggcca gatcctaagc acagtgcgtc attcgacatt
      301 tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg ataagcaggt aaatgcctct
      361 ttctttgctg agaatacggt tcaatcatta cttacctggg gagatcacga gcttgaggct
      421 gcgggacgtc tcactcagcc tttgaaatat gatgccgtca gcgactgtta caagccatta
      481 agctggcaac aagctttcga cgaaattggc gcacgccttc aaagctatag tgatcccaat
      541 caggttgaat tctatacttc gggccgcact tccaatgaag ctgcctttct ttatcagctt
      601 tttgcccgtg aatacgggag caataacttt cccgactgct ccaacatgtg ccatgaaccg
      661 accagtgtgg gtttggcagc gagtatcggc gtgggtaaag ggactgtgtt gctggaagac
      721 tttgagaagt gcgatttagt catttgcatt gggcataacc ctggtacaaa ccaccctcgc
      781 atgctgactt cgttgcgcgc tttagtgaaa cggggagcga aaatgatcgc catcaatcct
      841 ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa acccgtttga aatgctgacg
      901 aactctgaga ctcagttggc cagtgcctac tacaacgtgc gcattggtgg cgatatggcg
      961 ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg atgctgcaag tgccgcaggt
     1021 cggccctcat tgcttgatga cgaatttatt caaacgcata ccgtcggctt tgacgagcta
     1081 cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac gtatttctgg actaagtcag
     1141 acacaaatcg ccgaactggc tgacgcatat gccgctgccg atcgcaccat tatctgttac
     1201 ggaatgggga tcactcagca cgaacatggt acccagaatg tacagcaact ggtcaatctg
     1261 ctgttgatga aaggtaacat tggcaagcct ggtgcgggta tctgcccact acgaggtcac
     1321 tctaatgtac agggcgaccg aaccgtcggt atcaccgaga aaccgtctgc agagtttctg
     1381 gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg cacctggaca tgctgcaatt
     1441 gccagcatgc aagcaatatg cacggggcag gctcgagcat tgatctgcat ggggggcaac
     1501 tttgcactgg caatgccaga tcgggaagcg agcgctgtac cgttaacgca attagatctg
     1561 gcggtacacg tagccactaa gcttaaccgc tctcatctgc tgaccgcacg gcatagctat
     1621 attctgccgg ttctgggacg tagcgagatt gacatgcaaa aaagcggtgc gcaggcggta
     1681 accgttgagg attcaatgtc gatgattcat gcctcgcgtg gcgtgttaaa acccgccggt
     1741 gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg cgcaggcagc actaccccag
     1801 agcgtggtag cctgggagta tctggtggaa gattatgatc gcattcgcaa tgacattgaa
     1861 gctgtgctgc cagagttcgc cgactataac cagcgcatcc gacatcctgg aggttttcac
     1921 ctgataaatg cagctgctga aaggcgctgg atgacgccgt caggtaaggc taatttcatt
     1981 accagcaaag ggctgttaga agatccctct tcagcgttta acagtaagct ggtcatggcg
     2041 acagtacgca gtcatgatca gtacaacacg acgatttatg gtatggatga tcgctatcga
     2101 ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta aacaagctaa aatttgccgt
     2161 gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc cagacggtaa gcgcagctca
     2221 cgccgcatgg atagattaaa agtggtcatt taccctatgg ctgaccgctc actggtgacc
     2281 tattttccag aatcgaatca catgctaaca cttgataagc acgatccatt aagtggcatt
     2341 cctggctata aaagtattcc ggttgaatta gaaccatcaa attaatgcct cttctcattt
     2401 cttctgctgt catccgcaca gcagaagaat tcctcattga ctattatcgc gcaatttgct
     2461 cacatggatt aaattaaact acatactata agatataaac ttctgcctac agctgtaaga
     2521 aactccgctc agtactgaag caccagtcct atttcctctt ttttccagcc tgttatatta
     2581 agcatactga ttaacgattt ttaacgttat ccgagaaata aacatatttg aaatgcatgc
     2641 gaccacagtg aaaaacaaaa tcacgcaacg agacaactat aaagaaatca tgtctgtaat
     2701 tgtggttgtc ttattactga cacttacgtt gatagccatt ttttcggcaa ttgatcagct
     2761 gggtatttca gaaatgggtc gcattgcaag agatct
//
LOCUS       DAZKIV010000295         2755 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZKIV010000295 DAZKIV010000000
VERSION     DAZKIV010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2755
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.297"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..479
                     /locus_tag="ACHOT6_004825"
     CDS             3..479
                     /locus_tag="ACHOT6_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFP9949881.1"
                     /translation="MDGELKNMKLNINQLAALSGLHRQTVAARMADVPLAPGSNEKKK
                     LYLLTDLITSLLEKPPSSEDEDMDPHARKAWYQSERERLKFQHETVQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
     gene            476..1486
                     /locus_tag="ACHOT6_004826"
                     /pseudo
     CDS             476..1486
                     /locus_tag="ACHOT6_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            1684..>2755
                     /locus_tag="ACHOT6_004827"
     CDS             1684..>2755
                     /locus_tag="ACHOT6_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001452112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFP9949882.1"
                     /translation="MKPREKDYAVYDGFGLLLNVSKAGGKVWRFRYSHPITKKRQTYT
                     IGRFPEFSLAEAREVRDELRRMIARGVDPVTEKKNRKIEMSLKNLQTFEAVANAWFAF
                     KKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDAVSDKNALQKKLIS
                     RLNEIMNYAVNCGALKANPLLKIKTAFTGKKNKSLAALPVERLPEFLSWWDSVPHTYQ
                     IAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHREHVVPLSSQAISI
                     LRTMQEIKRGRYVFFSSRTKDAPMGRNTIKTPIAASKFKGIVTLHGFRSMWSTLLNEE
                     GFNPDVIEAALAHKSGDKIRDIY"
BASE COUNT          738 a          572 c          738 g          707 t
ORIGIN      
        1 aaatggacgg cgagctgaaa aatatgaagt taaatattaa tcaactggca gccctttcag
       61 gtctgcaccg gcagactgtt gccgccagaa tggcggatgt tcctcttgca ccaggcagta
      121 atgaaaagaa aaaactgtat ctcctgacgg atttgataac ttcgttgctg gaaaaaccac
      181 catcttccga agatgaggat atggatcctc acgctcgtaa ggcatggtat cagtccgagc
      241 gcgagcgtct taaatttcag catgaaactg ttcagcttgt gccagtcagt gatgtcaggc
      301 ggtccttttc tgtcgtggtg aaagcgatag ttcaggtact ggaaacctgg cctgaccggc
      361 tggagaggga cagggggtgg actgcatcac aactgaatga agtacagatt gtggttgatg
      421 agatccgcga cacactggaa aaggcagtca ttgactgttg tgatgaggcc gatatgtgaa
      481 tcaggtgaac gagagccata gccgcgcatc cgatatctgg cgcgaagtgg cctcgctgtt
      541 tcgcccaccc agccggttac cagtagcgga agccatcagg cgttatatgc gggttccacg
      601 gggagccaat acttccggtc cgtgggagtc atcgctgacg ccctatatga ttgaccccat
      661 taatacatta tcagcccgtg aatatgacgc ggtggtgttt gtgggacctg cgcgaaccgg
      721 gaaaaccgaa gggctgattg atggctggat tgtgtacggc atcatctgtg atccggcgga
      781 tatgctggtg gtgcagatga ctgagacgaa ggcgcgtgag cattccagaa cgcgtctttc
      841 caggacgttt cgccacagtc cggaggtcag caagcgcctc agtccttccc gtaatgacaa
      901 caacgtccac gataaaatgt ttcttgacgg ctccttcctg aagattggct ggccgtcgat
      961 caccgtcttt tcctcttcgg attaccgtcg tgtggcgctg acggattatg accgtttccc
     1021 tgaaaacgtg gacggggaag gggatgcctt cacgctggcc tcaaagcgta ccaccacctt
     1081 tatgtcttcg gggatgaccc tggtcgagag ttcaccgggg cgggatatca ccgataccaa
     1141 atggcgttgt ggtggcgcac atgaggcacc gccaacaacg gggatcctgt cactgtataa
     1201 ccggggagac cgccgccggt ggtactggcc gtgtccgcac tgcggggaat attttcagcc
     1261 ggtgatggat aacatgaccg gataccggaa taacccggat tttgtggctg ccggtcaggc
     1321 tgcccgtctg atgtgtccgc attgtcgcgg gctgattgcc cctgagcaga aacgcgaact
     1381 gaataaccag gggatctggc ttcgtgaagg tgaacgggcg gcggcggacg gcagtatcac
     1441 cggaacgcca cgaaactccc ggattgcgtc attttggatg gagggtacat ttcttacctg
     1501 tttgtatgtt ctggtgtcga ttcatagtct tttcaatgag ttgtgatttt atgagtttcc
     1561 tctctttgct tgataatgag ttagtttatc gcttgttatt ggcttgaatg gactacatga
     1621 cggactaaaa aatgagggcg atagatgtcg gtaaagccat taaccgtgac tgaagttaag
     1681 gggatgaaac cacgtgaaaa ggactatgcc gtttatgatg ggttcggttt attgctgaat
     1741 gtgagtaaag ccggtgggaa agtgtggcgt ttccgttata gccatccgat aacgaagaaa
     1801 cggcagacat acacgatagg acgttttcct gaattctcac tcgcggaagc acgggaagta
     1861 cgtgatgaac ttcggcgaat gattgcacgt ggagttgatc cagtgacgga gaagaaaaat
     1921 cgtaaaattg agatgtcact aaaaaatcta cagacatttg aagctgttgc taatgcatgg
     1981 ttcgctttta aaaagggatc tgaattgcgg aaacctacgc tgtataatat cgaatatgaa
     2041 gtatacaaat atcttgttcc tttctttggt aagtacagta tagaaaaaat tacagcacca
     2101 gtagctatta atgctctgga tgccgtatcc gataagaatg cgttgcaaaa aaaattaata
     2161 tcaagattaa atgaaattat gaattatgct gtaaattgtg gagcattgaa agcaaatcca
     2221 ttacttaaga taaagactgc attcacagga aagaaaaata aatcattagc agcactacct
     2281 gttgaaagat tgcctgaatt tctgagctgg tgggatagtg tgcctcatac ctatcaaata
     2341 gctcataatg cacttttatt ccagatattg acaatggtca ggccaggtga ggcgattaaa
     2401 gcagagtggt cagagattga ttttgattct ggcttgtgga ttatccccgc gcataaaatg
     2461 aaatgccatc gtgaacatgt tgttcccttg tcatcacagg ctattagtat cctcagaaca
     2521 atgcaggaaa taaaaagagg gcgttatgtg tttttttcct ccagaacaaa agatgcgcct
     2581 atggggagga atactatcaa gaccccaatt gctgccagta agttcaaagg gattgtaacg
     2641 ttacatggtt ttcgttcaat gtggagtacg cttttaaatg aggagggatt taaccccgat
     2701 gtaatcgagg ctgcattggc gcataaaagt ggtgataaaa taagagatat ttata
//
LOCUS       DAZKIV010000296         2632 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZKIV010000296 DAZKIV010000000
VERSION     DAZKIV010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.298"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..2632
                     /gene="rhsC"
                     /locus_tag="ACHOT6_004828"
                     /pseudo
     CDS             1..2632
                     /gene="rhsC"
                     /locus_tag="ACHOT6_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsC"
BASE COUNT          599 a          810 c          810 g          413 t
ORIGIN      
        1 ggatatccgc ttacagctgc gcgataacac actgatactc agtgataacg gcggcagaag
       61 cctgtatttt gagcacctgt ttcccggtga ggacggttac agccgcagcg agtcactgtg
      121 gctggtgcgc ggcggcgtgg cgaaactgga tgaaggtcac cggctggccg cactctggca
      181 ggcgctgccg gaagaactcc gcttaagtcc gcatcgttat ctggcgacaa acagtccgca
      241 gcggccgtgg tggctgctcg gctggtgtga gcgggtgccg gaagcggatg aggtgctgcc
      301 tgcgccgctg ccgccgtacc gggtactgac cgggctggtg gaccgcttcg ggcgcacaca
      361 gacgttccac cgcgaagccg ccggtgaatt cagcggcgaa atcaccggcg tgacggatgg
      421 tgccgggcgt cacttccggc tggtactgac cacgcaggcg cagcgggcag aagaagcccg
      481 gcagcaggcc atttccggcg ggacggaacc gtccgctttt cctgataccc tgccgggtta
      541 caccgaatat ggccgggaca acggcatccg tctgtctgcc gtgtggctga cgcacgaccc
      601 ggaatacccg gagaatttac ctgccgcgcc gctggtgcgc tatggctgga cgccgcgcgg
      661 cgaactggcg gcagtgtatg accgcagcaa tacacaggtg cgcagcttta cttacgatga
      721 taaataccgg ggccggatgg tggcgcaccg tcacacgggc cgaccggaaa tccgttaccg
      781 ttacgacagc gacgggcggg tgacagaaca gctaaacccg gcaggcttaa gctacacgta
      841 tcagtatgag aaggaccgca tcaccatcac cgacagcctg aaccgccgtg aagtgctgca
      901 cacgcagggt gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt
      961 cacgcagagt cagtttgatg cggtgggcag gcttaaggca cagacggatg ccgcaggcag
     1021 gacaacagag tacagcccgg atgtggtgac gggcctcatc acgcgcatca ccacgccgga
     1081 tggcagggca tcggcgtttt actataacca ccacagccag ttaacgtcag ccaccgggcc
     1141 agacgggctg gaattgcgcc gggaatatga tgagtacgga cgtctgattc aggaaaccgc
     1201 ccctgacggc gatatcaccc gctaccgtta tgataatcca cacagtgacc tcccctgcgc
     1261 aacggaagat gccaccggca gccggaaaac catgacgtgg agccgttacg gtcacagttg
     1321 ctgaccttca ctgactgttc cggttatgta acccgctatg accatgaccg ttttggtcag
     1381 gtgacggcgg tgcaccgcga ggaaggcctg agccagtacc acgcatacga cagccgtgga
     1441 cagttaattg ccgtgaaaga cacgcagggc catgaaacgc ggtatgaata caacgccgcc
     1501 ggtgacctga ccaccgtcat tgccccggac ggcagcagaa acgggacaca gtacgatgcg
     1561 tggggaaaag ccatctgtac cacgcagggc ggtctgacgc gcagtatgga atacgatgct
     1621 gccggacggg tcatccgcct gaccagtgaa aacggcagcc acaccacctt ccgttacgat
     1681 gtactcgacc ggctgataca ggaaaccggc tttgacggcc gcacacagcg ttatcaccac
     1741 gacctgaccg gcaaacttat ccgcagcgag gatgaggggc tggtcaccca ctggcactat
     1801 gacgaagcag accgcctcac gcaccgcacc gtgaatggcg aaaccgcaga gcagtggcag
     1861 tatgacgaac gcggctggct gacagacatc agccatatca gcgaagggca ccgggtgacg
     1921 gtgcattacg ggtatgatga gaaaggccgg ctgaccggtg agcgtcagac ggtgcatcac
     1981 ccgcagacgg aagcactgct ctggcagcat gagaccagac atgcgtacaa cgcgcaggga
     2041 ctggcgaacc gctgtatacc ggacagcctg cccgccgtgg aatggctgac ctacggcagc
     2101 ggttacctgg caggcatgaa actcggcgac acaccgctgg tggagtacac ccgcgaccgc
     2161 ctgcaccggg aaacgctgcg cagcttcggc cgttatgaac tcaccaccgc ttatacccct
     2221 gccgggcagt tacagagcca gcacctgaac agcctgctgt ctgaccgcga ttacacctgg
     2281 aacgacaacg gcgaactcat ccgcatcagc agcccgcgcc agacccggag ttacagctac
     2341 agcaccaccg gcaggctgac cagcgttcac accaccgcag cgaatctgga tatccgcatc
     2401 ccgtatgcca cagacccggc aggtaaccgc ctgcccgacc cggagctgca cccggacagc
     2461 accctcagca tgtggccgga taaccgtatc gcccgtgacg cgcactatct ttaccggtat
     2521 gaccgtcacg gcaggctgac agagaaaacc gacctcatcc cggaaggggt tatccgcacg
     2581 gatgatgagc ggactcaccg gtaccattac gacagtcagc accggctggt gc
//
LOCUS       DAZKIV010000297         2627 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZKIV010000297 DAZKIV010000000
VERSION     DAZKIV010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.299"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..680
                     /gene="tnpA"
                     /locus_tag="ACHOT6_004829"
     CDS             3..680
                     /gene="tnpA"
                     /locus_tag="ACHOT6_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFP9949883.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            680..1027
                     /gene="tnpB"
                     /locus_tag="ACHOT6_004830"
     CDS             680..1027
                     /gene="tnpB"
                     /locus_tag="ACHOT6_004830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9949884.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1047..2618
                     /locus_tag="ACHOT6_004831"
     CDS             1047..2618
                     /locus_tag="ACHOT6_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISCro1 family transposase"
                     /protein_id="HFP9949885.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKQACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTYITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQQQMKTLSRHSDTAKAFAYLLKQWDALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          633 a          719 c          734 g          541 t
ORIGIN      
        1 taatgtccat catttttaat ggacactatc gtatgaaaca ccggacctgg atcactgaag
       61 ctttacgtct tcactttgaa gaacatttac cccgggttgt ggccgggcgt cgcctgggtg
      121 taccaaaatc aacagtttgt agtatgttcg tgcgctttcg gagagctggc ctttcgtggc
      181 ctttgcccgc aggcatgtcg gagcaggaac ttgatgcctg cctttacgga caattttcca
      241 cggtaccagt cgtacgtcct gaaagcaccg ttatatccga agcccccgtg gtaaaaaaac
      301 gtccccggcg gcccaacttc ccttatgagt ttaaaatcgc cttagtggag cagtcactgc
      361 agcccggagc ctgtgtggcg cagatcgccc gggaaaacgg aatcaacgat aacctgctct
      421 tcaactggcg ccatcaatac cggaaaggtg gcctgctgcc ttccggaaaa aatatgccgg
      481 cactgcttcc cgtgacgtta acgccggagc cggataataa aatcccggcc cccgcacagg
      541 aaccagagca gataaataca ccgtccgaca gtctgtgttg tgagctggtt ctgccggccg
      601 gaactctcag gcttaaaggt aaactgacgc cggcgttatt acagacactt atccgcgaaa
      661 taaaagggag cagccactga tgatatctct ccctgcaggt tcgcgtatct ggctggttgc
      721 aggtatcacc gatatgcgaa atggctttaa cggcctggca tcaaaagttc agaacgtcct
      781 gaaggatgac ccgttctccg gacacctgtt catcttccgc ggacgccggg gtgaccagat
      841 aaaagtgttg tgggctgaca gtgacggact gtgcctcttc accaaacgcc tggagcgggg
      901 ccgcttcgtc tggccagtca cccgtgacgg caaggtgcac cttactccgg ctcagttatc
      961 catgcttctt gaaggtatca actggaagca cccgaaacga acggaacgcg ctggaatccg
     1021 catataaccc gttgtaaagt gaggatatgg acacctcact tgctcatgag aacgcccgcc
     1081 tgcgggcact gttgcagacg caacaggaca ccatccgcca gatggctgaa tacaaccgcc
     1141 tgctctcaca gcgggtggcg gcttatgctt ccgaaatcaa ccggctgaag gcgctggttg
     1201 cgaaactgca acgtatgcag ttcggtaaaa gctcagaaaa acttcgtgca aaaaccgaac
     1261 ggcagataca ggaagcacag gagcgaatca gcgcacttca ggaagaaatg gcggaaacgc
     1321 tgggtgagca atatgacccg gtactgccat ccgccctgcg ccagtcttca gcccgtaaac
     1381 cgttaccggc ctcacttccc cgtgaaaccc gggttatccg gccggaagag gaatgctgtc
     1441 ctgcctgtgg tggtgaactc agttctctgg gatgtgatgt gtcagagcaa ctggagctta
     1501 tcagcagcgc ctttaaggtt atcgaaacac aacgtccgaa acaggcctgt tgccggtgcg
     1561 accatatcgt gcaggcacca gtaccttcaa aacccattgc acgcagttat gccggagcgg
     1621 ggcttctggc ccatgttgtc accgggaaat atgcagacca tctgccgtta taccgccagt
     1681 cagaaatata ccgtcgtcag ggagtggagc tgagccgtgc cacactgggg cgctggacag
     1741 gtgctgttgc tgaactgctg gagccgctgt atgacgtcct gcgccagtat gtgctgatgc
     1801 ccggtaaagt ccatgctgat gatatccccg tcccggtcca ggagccgggc agcggtaaaa
     1861 cccggacagc ccggctgtgg gtctacgtcc gtgatgaccg taacgccggt tcacagatgc
     1921 ccccggcggt ctggttcgcg tacagtccgg accggaaagg tatccatcca caaaatcacc
     1981 tggccggtta cagcggtgtg cttcaggccg atgcttacgg tggttaccgg gcgttatacg
     2041 aatccggcag aataacggaa gccgcgtgta tggctcatgc ccggagaaaa atccacgatg
     2101 tgcatgcaag agcgcccacc tacatcacca cggaagccct gcagcgtatc ggtgaactgt
     2161 atgccatcga ggcagaggtc cggggctgtt cagcagaaca gcgtctggcg gcaagaaaag
     2221 ccagagccgc gccactgatg cagtcactgt atgactggat acagcaacag atgaaaacac
     2281 tgtcgcgtca ctcagatacg gcaaaagcgt tcgcatacct gctgaaacag tgggatgcac
     2341 tgaacgtgta ctgcagtaat ggctgggtgg aaatcgacaa caacatcgca gagaacgcct
     2401 tacggggagt ggccgtaggc cggaaaaact ggatgttcgc gggttccgac agcggtggtg
     2461 aacatgcggc ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggagccag
     2521 aaaagtggct gcgttacgtc attgaacata tccaggactg gccggcaaac cgggtacgcg
     2581 atctgttgcc ctggaaagtt gatctgagct ctcagtaaat atcaata
//
LOCUS       DAZKIV010000298         2601 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZKIV010000298 DAZKIV010000000
VERSION     DAZKIV010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.300"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(68..151)
                     /locus_tag="ACHOT6_004832"
                     /pseudo
     CDS             complement(68..151)
                     /locus_tag="ACHOT6_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374575.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
     gene            complement(751..1185)
                     /gene="uspF"
                     /locus_tag="ACHOT6_004833"
     CDS             complement(751..1185)
                     /gene="uspF"
                     /locus_tag="ACHOT6_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFP9949886.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            complement(1326..2459)
                     /gene="ompN"
                     /locus_tag="ACHOT6_004834"
     CDS             complement(1326..2459)
                     /gene="ompN"
                     /locus_tag="ACHOT6_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFP9949887.1"
                     /translation="MKSKVLTLLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GQVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
BASE COUNT          722 a          549 c          605 g          725 t
ORIGIN      
        1 gttgatgatg ggcaatcgtg caacattgta ttgtatgtaa aggcagagcc ccgaatttta
       61 ctgttgtaag tgggcctggc gggaatggta tcgccagcat cattggcttg ggtccagtaa
      121 aaagagacgc agaaagatgc acaataggca ctacgcgtca tgcatggatt aggtcgctca
      181 tcatttacac agtactgcta aaaaattatt aaggaaagcg cggcacttcg tatgtaagaa
      241 cgtgccgcgg ttggctggta agcttttcga tagtgcgagt atctaatgat ttccagccgg
      301 aactgattct attggaaaga aatcatgggc gcaatttaca gatcacagat tgattagtta
      361 tagattttta ctgtggcaaa aatgggacaa aaggccgcaa aaggggcaaa aatggggcaa
      421 aaaaagtgta gattattgtg ctttattgta gttgttaatc ctgcctaaca tattgaaaaa
      481 cataaaagtt attgttcgtc agatagttgc ggtttttact ctgacttgtt caggttgtat
      541 tgttctttct tactaatttc ttgattttgc gacatttaaa agcgactcaa ttcgttatat
      601 ggcatcagaa gagtatgcgt catgccggaa cgccgagcat aagaaatctg atataaaaac
      661 tgtggcgtgt atggtacgga ttagagggga aaatgtcagc acatttgcga aatgaatcaa
      721 aaagcccgca gcaatgtgcg ggcgttagtg tcagcgcaca accagcacgg agcactctgc
      781 gtgacgcact acagctgcgg cgttggaacc gagcagataa gtggtgatat ccggtcgatg
      841 ggaagcaatg atgatcatat gagcggggat cttcttcgcc aattccagaa tgcggtcttt
      901 gggcgagcct tcctcaacat ggacatgcac tctgtcggtt ggcagtttaa attttttaat
      961 gatctcttcc agttgcgatt tggcttccgc tttcaggtca tccattgccg gtaattctgc
     1021 ggaatacgct aaacccagag aggcatagta gggcagtgaa ggtattaccg tcaggaaatg
     1081 aacctctgca tcatcaatct ttgcctctgc ctcaacgtgg ctaatcacgc gttgagttaa
     1141 ttctgaatcg gaaatatcga tagggacaag aatcgttctg ttcataaaac ctcctgtttt
     1201 agtatccgca taaagtgtaa cgccagatga cactttttgt gtaatgacgg agttcacatt
     1261 tttaatttag atcaaaggag gaagaataag cagaaaaagc ccgccataac agcgggcagg
     1321 aggatttaga actgataaac cagacctaaa gcgacaatat catcggtaga gatgccgttg
     1381 gcagcgtaga aactgtcatc ttcatccaac aggttgattt tatagtcaac ataggtggac
     1441 atgtttttat tgaaatagta agtcgcgcca acatcggcgt atttaaccag atctttatca
     1501 tcaacacctg ccgggttgtc tgcaccaccg gcagcgtgca ggtcacggcc tttagacatc
     1561 aggaaagaga ctgccggacg cagaccaaaa tcaaactggt actgtgcagt gacttcaaag
     1621 ttctgggttt tgttcgcaac tgcataatcg ctgtcgccaa acggggtcat attacgcgtt
     1681 tctgaataca tggttgccag gtaaatattg ttagcatcgt attttagccc agcagtccac
     1741 gcgtctgctt tatcaccacc cgccgcagta tggttaacct ggtcattggt gcggtcagaa
     1801 gaggtgtatg ccgcaccagc gctaaagccc atgcctaaat catatgttgt ggaaagaccc
     1861 cagccgtcac cgttttcatg gcgaacatca cgtccgttgt tggtgccttc ctgaccatta
     1921 ctggcacctt cgttgttacc ttgatactgc accgcgaagt tcagaccatt tacctgaccg
     1981 aagaaatcag tattacgata agtcgcgacg ccgttggcac gaccagtcat aaagttgtct
     2041 gcattggtat aagagtcacc gccaaattca ggcagcatat cggtccagcc ttcgatgtcg
     2101 tacattacgc cataattacg tccgtaatcg aaagaaccgt aatctgcaaa tttcagtccg
     2161 gcaaatgcca gacgggtcca tgactggttt tttgaagatt cagtgttgtt tgcctgaata
     2221 ttgtattccc attgaccgta gccagtgagt tgatcgttaa tttgggtttc gcctttaaaa
     2281 cccagacgcg catagctctg gtcgccatct ttcgctgaat tatcagaaaa ataatgcagg
     2341 ccatcaactt tgccatacag atctaatttg ttgccgtctt tattataaac ttcggctgca
     2401 tgtgcagcac ctgcggcgag cagggcagga attaaaagtg tcagtacttt gcttttcatt
     2461 gaataaatcc tttagttatt ttatttgcct tttatcccca tttcggggag tgaaaacatc
     2521 ctaaaggaga agttcagtta gccgataaat atcatttgtt acgccttaag taaaacctta
     2581 atcaaataat tcctctgaaa t
//
LOCUS       DAZKIV010000299         2588 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZKIV010000299 DAZKIV010000000
VERSION     DAZKIV010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.301"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            313..1977
                     /gene="asnB"
                     /locus_tag="ACHOT6_004835"
     CDS             313..1977
                     /gene="asnB"
                     /locus_tag="ACHOT6_004835"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFP9949888.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2358..2434
                     /locus_tag="ACHOT6_004836"
     tRNA            2358..2434
                     /locus_tag="ACHOT6_004836"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2392..2394,aa:Met,seq:cat)
     gene            2443..2527
                     /locus_tag="ACHOT6_004837"
     tRNA            2443..2527
                     /locus_tag="ACHOT6_004837"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2477..2479,aa:Leu,seq:tag)
BASE COUNT          678 a          634 c          642 g          634 t
ORIGIN      
        1 tcctgcaata tattgaattt tcatgctttt gtaggccgga taaggcgttc acgccgcatc
       61 cggcatgaac aaatcgcact ttatcagcaa tttggccccc gtaaaatata accattcaat
      121 aaaacccgct aaaaattatc gattcatcaa taaatacatc ctcacaccag accctttttt
      181 caccaatcga caatcaccat tacgtttttt attttttcac cgccaaatcg cttgcgcaca
      241 gtgctgcttt gcggcatttt tttaaacaag caaacacaac aagcaacaaa taccaggtta
      301 acggagaagg ttatgtgttc aattttcggc gtattcgata tcaaaacaga cgcagttgag
      361 ctgcgtaaga aagcactcga gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc
      421 ggtatttatg ccagcgataa cgccattctc gctcacgaac gtctgtcaat tgttgacgtt
      481 aacgcggggg cgcaacctct ctacaaccaa caaaaaaccc acgtactggc ggtaaacggt
      541 gaaatctaca accaccaggc attgcgcgct gaatatggcg atcgttatca gttccagaca
      601 gggtctgact gtgaagtgat cctcgcgctg tatcaggaaa aagggccgga atttctcgac
      661 gacttgcagg gcatgtttgc ctttgccctg tacgacagcg aaaaagatgc ctacctgatt
      721 ggtcgcgacc atctggggat catcccactg tatatgggct atgacgaaca cggtcagctg
      781 tatgtggcct cagaaatgaa atcgctggtg ccagtttgcc gcacgattaa agagttcccg
      841 gcggggagct atttgtggag ccaggacggc gaaatccgtt cttattatca tcgcgactgg
      901 ttcgactttg atgcagtgaa agataacgtg accgacaaaa acgagctgcg tcaggctctg
      961 gaagattccg ttaaaagcca tctgatgtct gatgtgcctt acggtgtgct gctttctggt
     1021 ggtctggatt cctcaattat ttccgctatc accaagaaat acgcagcccg tcgcgtggaa
     1081 gatcaggaac gctctgaagc ctggtggccg cagttacact cctttgctgt aggtctgccg
     1141 ggttcaccgg atcttaaagc agcccaggaa gtggcaaacc atctgggcac ggtgcatcac
     1201 gaaattcact tcactgtaca ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc
     1261 gaaacttatg atgtgaccac tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag
     1321 atcaaggcga tgggcattaa aatggtgctg tccggtgaag gttctgatga agtgtttggc
     1381 ggttatcttt acttccataa agcgcccaac gctaaagaac tgcatgaaga gacggtgcgt
     1441 aaactgctgg ccctgcatat gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg
     1501 ggcgtggaag cacgcgttcc gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt
     1561 aacccgcagg ataaaatgtg cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt
     1621 tttgagtcat acctgcccgc aagcgtggcc tggcggcaga aagagcagtt ctccgatggc
     1681 gtcggttaca gttggatcga caccctgaaa gaagtggcgg cgcagcaggt ttctaatcag
     1741 caactggaaa ctgcccgctt ccgcttcccg tacaacacgc caacctcaaa agaagcgtat
     1801 ctgtaccggg agatctttga agaactgttc ccgcttccga gcgccgctga gtgcgtgcct
     1861 ggcggtcctt ccgtcgcgtg ttcttccgct aaagcgatcg aatgggatga agcgttcaag
     1921 aaaatggacg atccatctgg tcgtgcggtt ggtgttcacc agtcggcata taagtaagaa
     1981 atgcgaaaca gccccggtga aatccggggc tgtttcagtt attgaggtgc ttgttgattc
     2041 tgatatgcag taaggaaatg ttccgggttt gccacatcac tgtttgacaa ctcatcactg
     2101 taggccagta tttcatagat taatttccca ccactattgt tgaattccga atttcatcct
     2161 gtacgacaaa agaatggctg tagggtgggg aaaaaacaca aagtaaacaa taattgacga
     2221 atatagcgcc acgctgttcg caacctaacc aaacagtcac tttcgagcaa ttttccttga
     2281 aaaagaggtt gacgctgcaa ggctctatac gcataatgcg ccccgcaacg ccgataaggt
     2341 atcgcgaaaa aaaagatggc tacgtagctc agttggttag agcacatcac tcataatgat
     2401 ggggtcacag gttcgaatcc cgtcgtagcc accatctttt ttgcgggagt ggcgaaattg
     2461 gtagacgcac cagatttagg ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc
     2521 cgcaccattc accagaaagc gttgtacgga tggggtatcg ccaagcggta aggcaccggt
     2581 ttttgata
//
LOCUS       DAZKIV010000300         2552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZKIV010000300 DAZKIV010000000
VERSION     DAZKIV010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.302"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            170..1401
                     /locus_tag="ACHOT6_004838"
                     /pseudo
     CDS             170..1401
                     /locus_tag="ACHOT6_004838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter substrate-binding
                     protein"
     gene            1404..2552
                     /locus_tag="ACHOT6_004839"
                     /pseudo
     CDS             1404..2552
                     /locus_tag="ACHOT6_004839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243614.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
BASE COUNT          753 a          467 c          517 g          815 t
ORIGIN      
        1 tttctcaaca aattactgca aatgggatat gcatcactat ttgcagttat tcattattaa
       61 aatcttattt gacagttgat tggccttgat ttgtgttgat aatggcgtga atgcttatgt
      121 agtcgtacag catgtgctac ttaaagatgt tattcatgag gtaaatataa tgaagctttc
      181 aaatatcgtc acagttataa ttcttgcaat ttccagtacg ctaacacccc aggcgatggc
      241 agaaaaatta attcctgaaa ctgatgctga gttgttagtt tggtctgatg ccacaagcgc
      301 ggaatatatg aaatatgcag caaaggaatt caataaagat tttggctaca aggtaaagtt
      361 tacatttcgc aatatagcgc caatggatgc agcatcaaga attatgcagg atgggggtac
      421 gactcgtgta gctgatgtag ctgaaattga acatgatacc ctggggcggt tagtcgttgc
      481 tggcggggtt atggaaaaca tggtctcagc tgagcgcatt aaaaaacatt tattccaggc
      541 gcagtatcgg cagctacata taataacatc agctatggtt ttcctgtaag tttcgcaacg
      601 ctggcgcttt tctataataa ggatttgtta aacaccgcac caaaaacatt cgaagaaatc
      661 aatactttca gtgaaaagtt taataattca tccgagcata aatatgctct gctatgggat
      721 gtacaaaatt attatgtttc acgtatgttt attaccttgt atggtgccaa cgaattcggt
      781 aaaaccggta acgatcctaa agctctaggc atcgcttcat ctgaagcgaa gaaagggcta
      841 gagacgatga aacgcttaaa gaaagcgaat ccctctaatc ctcttgatat gggtaatcca
      901 caagttctaa gaggtctgtt taatgaaggt aaagttgctg ctgtaatcga cggaccttgg
      961 tccatacaag gttacattga cagcggaatc aattttggcg tgacacgcat cccaacatta
     1021 gatggtcatc agcctcgcac attttcaaca gtacggctgg ccgttgtaag ctcatttacc
     1081 gaatatcctc atgccgctga attattcgct gactacctga caacggataa gatgttgatg
     1141 aagcgttatg aaatgacgaa tctcatccca ccaatcaatt cacttatgaa caaaattagc
     1201 cagacaggta gtgaagctat aaaagcaatt attgctcaag ctaattattc tgacgcaatg
     1261 ccatcaatac cggaaatgtc ttatctatgg tctccaatga ctaatgctat tttggctacc
     1321 tgggttgaga ataaaacacc ggatgaagtt ttaaatcatg cacaaacaat tattgaagaa
     1381 caactttcgc ttcaggagta attgtgaata cacaacaact gaaaatgaag agtgcacctg
     1441 tgctacctat ttcctgtttg attatgggcg ggactcagtt atcacgtcat tattatgtga
     1501 aaggcgggat attctttgca atacaagtat gctttttgct ttatctttcc gatattgtgc
     1561 atacattaat aggtttgttc acattaggtg atgtcgcgca aattcgaaaa gggttgactg
     1621 ttattcaggg tgacaattct atatttatgc ttgtagaggg cgtgatagcg accattattg
     1681 ttggcttatt cgccactatt tacatcctta atattaaaga tgcgcgaaac tcatcttatt
     1741 gccatctgac ttttaaacaa cagttatata agctctatga ggataaattt gcatttatag
     1801 tcctaacgcc cgcattttta gcgagtatag cattcattgt tttacctatt gtaattacag
     1861 ttcttgtatc gtttacaaac tatgctgcgc caaatcacat cccaccaaag aacctggttg
     1921 attgggttgg gattaaaaat ttcataatgt tgtttaaatt taaaatatgg tctgatacgt
     1981 ttctaggtgt cgctctctgg acatttattt gggcaatttg cgcgacgatt tttaccttca
     2041 gttttggctt tattctggct ttagcactgg ctaaaaaaat atacgtttcg caaaagtctg
     2101 gcgattgata tttatactgc catatgccat acccacattt gtaacgttac ttattttccg
     2161 tcttctttta aatggaattg gtccggttaa ctcgtttctg aacagtatcg gaatgaattc
     2221 agttgatttt ctgtcggttc cattaaatgc aaaaataaca gtaattgccg tttgtgtttg
     2281 ggtaggggca ccgtatttta tgctattgat cgcgggtgca ttaacaaaca tatcccgaga
     2341 tttgtatgag gctagtgagg tcgatggcgc aactaaatgg caacagttta aggaaattac
     2401 attgccaatg gtgttacatc aggttgcgcc aactctggta atgacatttg catataattt
     2461 caataatttt ggtgcaattt atcttctgac tgaaggtggt ccgactaatc cggaatacag
     2521 attcgctggg cataccgata tttttattac ct
//
LOCUS       DAZKIV010000301         2547 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZKIV010000301 DAZKIV010000000
VERSION     DAZKIV010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.303"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(80..922)
                     /locus_tag="ACHOT6_004840"
     CDS             complement(80..922)
                     /locus_tag="ACHOT6_004840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFP9949889.1"
                     /translation="MSDITISRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKAAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQTGRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(1007..1204)
                     /locus_tag="ACHOT6_004841"
     CDS             complement(1007..1204)
                     /locus_tag="ACHOT6_004841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFP9949890.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(1216..1704)
                     /locus_tag="ACHOT6_004842"
     CDS             complement(1216..1704)
                     /locus_tag="ACHOT6_004842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFP9949891.1"
                     /translation="MKLALTLEADSINAQTLNMGRIVVDVDGVNLTELINKVAENGYS
                     LRVVDDSDQQSTCTPPPLTTLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKMFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     NW"
     gene            complement(1701..2077)
                     /gene="cbtA"
                     /locus_tag="ACHOT6_004843"
                     /pseudo
     CDS             complement(1701..2077)
                     /gene="cbtA"
                     /locus_tag="ACHOT6_004843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            complement(2167..2532)
                     /locus_tag="ACHOT6_004844"
     CDS             complement(2167..2532)
                     /locus_tag="ACHOT6_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFP9949892.1"
                     /translation="MSDTLPGTTHPDNNNRPWWGLPCTVMPCFGARLVQEGNRLHYLA
                     DRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEADT
                     LGSCGYVYLAVYPTPETKK"
BASE COUNT          583 a          624 c          675 g          665 t
ORIGIN      
        1 tttgtttggc ttgattaaaa atgtaccctc aattataccc gcagtccctt ttgatatcga
       61 aagggaatca cactcgggat caggctgaga gcacagaagg aaagtacctg gctattatat
      121 cgttaattct gtcaatcagt tccagccttt tgaaagtgat gtgtgcagat cccttcttaa
      181 agtatttaat gatgaacatc tcgtcttcat agcgctcttt accctgaacg gactggatgt
      241 gaccatccag acgaatcgtg atgttttccc ggttgtcggg gatcggtttg ccgctgaaca
      301 gatgtagcat tctttccagg tcgacgagtc ggccagtctg ttgcccggtg ataagatgaa
      361 acccccaccg gtcccacctc accaggttgt tgacgataat tttactgcca aatttgcagg
      421 ggcaattggt tttgtaattc cagttcagtc ccctgaagac gttgatcacc cctcgctcaa
      481 acacctcatc cttattctgg tgtagttgtt caaatgtgct cagaatgttc gcttcactga
      541 tttccggaaa attatcgtac tccagtgacc ggtaccatgt atcacgggcc tgtgcatcca
      601 ttaaggaaag catacctgaa cgttgcatca ggtcacgcca gatttcgcga tcgagattgc
      661 gtgtgatggc tttcattgcg gtttccggtt tctccatcag ccagcagccg cagcgaaaat
      721 cctgtttcat ggcccagtcc ggggcggctt taccgccaat actttctgtc attgctgata
      781 tttcagccag ttgccggata agtgtgtcga tttgcagtag tgtactcttt cgtacagcca
      841 gaatgtggcg gatactggtt gagcagataa cgtccgtatg cccggtgacc acttccggac
      901 gggagattgt gatgtctgac attatcggtt tcccttttgg tttataaacg aaaacgccag
      961 ccctgttcag gctgacgtca gtggaatgaa gtatggtgag tgatatttac cggttctggt
     1021 gcagaagctg cagatgacga agagtacgga catcctcccg ggcctgttcg atacagggca
     1081 acagtgctgc cgaatccgtt ttatcctcat cgttgtcaaa gataattccc gattcgcagt
     1141 ctatattgtc ctgcagccac gcaatgagga tatccagcgc tgtttcagtg gttaatgatt
     1201 tcatgttgtt tctggttacc agttgaaagt aggtaaaccc ggcaggcatt cagcgctggc
     1261 atccagatga atgagactga cgccataacg ccggataagt gtgacaacca gacggcggaa
     1321 catttttgac agccccaggc gtttaagccg cagaaccggg tatgaccacg catcggtacg
     1381 taacagatat ccgctacctg taaaatgaat ccattctgat tcgccgaagt cactggtctg
     1441 gtgtgacagc gagtacaacc aggcgttgtc tgtttccgtg atatgtgcgg tgctgcagcg
     1501 tatgccggta agggttgtta acggtggtgg tgtgcaggtt gactgttggt cggagtcgtc
     1561 caccacacgg agtgaataac cgttttcagc gaccttgtta atcagttcag tgagattaac
     1621 accatcgacg tcaacgacaa tgcgccccat gttcagcgtc tgcgcattaa tgctgtcggc
     1681 ttccagcgtc agggccagtt tcattgtttc gcctccggat acttacccag ggtaatgtta
     1741 tttaccgttc tgtaattgtc gcgggtcatc aggccggtcg ccctgcgagc caggaggata
     1801 tcgatgctgt ttattaactg agagcgggta caggcgctga atcccggctg gtcggtagca
     1861 ccagcgcgta tttttccacg agaaagttca ccgcatcaca cagtgaaatg cctgcctcaa
     1921 tatgctgctc aatcacacgt tcatcggcaa acggtgtgtc attcagtgtg aggccatagt
     1981 gctggtccag cagtcgggac agcagtatct gccagatttc aacaggagac gggcgagaac
     2041 tagccgcctg cccgggtaat acaggtaatg ttttcatact gaagattttc ctgatatgca
     2101 gagataaaaa acgggacagt ggcgtggtga aaacaccagg ccggagcaga gggttattcc
     2161 ggtggtttac tttttcgttt cgggcgtcgg ataaacagcc agataaacgt aaccacagga
     2221 gcccagggta tcggcttcgc aggtcagtcc tttcgcatac agcgtgacgg tatgctgata
     2281 gcgggggctg agttcaccac tggtgagcat gagctccagt tgtttcatca gcagcggaaa
     2341 ggcctggtcc aggtggtacg aatccgcgtc gctgaaccgg cctctgatac cggcgcggtc
     2401 tgcaaggtaa tgcaaccggt taccctcctg caccagacgt gccccgaaac agggcatcac
     2461 ggtgcagggt agcccccacc aggggcggtt gttgttgtcg ggatgcgttg tcccgggcag
     2521 tgtgtctgac acgataaagt cctcaca
//
LOCUS       DAZKIV010000302         2543 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZKIV010000302 DAZKIV010000000
VERSION     DAZKIV010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.304"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..1463
                     /gene="glpT"
                     /locus_tag="ACHOT6_004845"
     CDS             105..1463
                     /gene="glpT"
                     /locus_tag="ACHOT6_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFP9949893.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            1468..>2543
                     /gene="glpQ"
                     /locus_tag="ACHOT6_004846"
     CDS             1468..>2543
                     /gene="glpQ"
                     /locus_tag="ACHOT6_004846"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFP9949894.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFSEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
BASE COUNT          609 a          543 c          713 g          678 t
ORIGIN      
        1 ttatgaatgt tttcttaaca tcgcggcaac tcaagaaacg gcaggttctc tcactgaatc
       61 aggctgttaa tcataaataa gaccacgggc cacggaggct atcaatgttg agtattttta
      121 aaccagcgcc acacaaagcg cgcttacctg ccgcggagat cgatccgact tatcgtcgat
      181 tgcgctggca aattttcctg gggatattct ttggctatgc ggcttactat ttggttcgta
      241 agaactttgc gcttgctatg ccttatctgg ttgagcaggg attctcacgc ggtgatttag
      301 gttttgccct ttcggggatc tcgattgctt atggattttc gaaattcatc atgggttcgg
      361 tatcggatcg ctcgaatccg cgcgttttcc tgcccgcagg tttgattctg gcggcggcag
      421 tgatgttgtt tatgggcttt gtgccatggg cgacgtcgag cattgcggtg atgtttgtac
      481 tgttgttcct ctgcggttgg ttccagggga tggggtggcc gccgtgtggt cgtactatgg
      541 tgcactggtg gtcgcagaaa gaacgtggcg gcattgtgtc agtgtggaac tgtgcgcaca
      601 acgtcggtgg tggtattccg ccgctgctgt tcctgctggg gatggcctgg ttcaatgact
      661 ggcatgcggc gctctatatg cctgctttct gcgccattct ggtggcatta ttcgcctttg
      721 cgatgatgcg cgataccccg caatcctgtg gcttgccgcc gatcgaagag tacaaaaatg
      781 attatccgga cgactataac gaaaaagcgg aacaggagct gaccgcgaag caaatcttca
      841 tgcagtacgt actgccgaac aaactgctgt ggtatatcgc catcgccaac gtgttcgttt
      901 atctgctgcg ttacggcatc ctcgactggt caccgactta tctgaaagag gttaagcatt
      961 tcgcgctgga taaatcctcc tgggcctact tcctttatga atatgcaggt attccgggca
     1021 ctctgctgtg cggctggatg tcggataaag tcttccgtgg caaccgtggg gcaaccggcg
     1081 ttttctttat gacactggtg accatcgcga ctatcgttta ctggatgaac ccggcaggta
     1141 acccaaccgt cgatatgatt tgtatgattg ttatcggctt cctgatctac ggtcctgtga
     1201 tgctgatcgg tctgcatgcg ctggaactgg caccgaaaaa agcggcaggt acggcagcgg
     1261 gctttaccgg gctgtttggt tacctgggcg gttcggtggc ggcgagcgcg attgttggct
     1321 acaccgtgga cttcttcggc tgggatggcg gctttatggt aatgattggc ggcagcattc
     1381 tggcggttat cttgttgatt gttgtgatga ttggcgaaaa acgtcgccat gaacaattac
     1441 tgcaaaaacg caacggaggc taatggcatg aaattgacgc tgaaaaacct tagcatggcg
     1501 atcatgatga gcactatagt catgggaagc agtgcaatgg cggcggacag caacgaaaaa
     1561 atagtcatcg cccatcgcgg tgccagtgga tatttgccgg agcatacgct gccagcaaaa
     1621 gcgatggcgt atgcgcaggg agcggattat ctggaacagg atttggtgat gaccaaagac
     1681 gaccatctgg ttgttctgca tgaccattac ctcgatcgtg ttactgatgt tgccgatcgt
     1741 ttcccggatc gggcgcgcaa agacggtcgt tactacgcga tagatttcac gctggatgaa
     1801 attaagtcgt tgaaatttag cgaaggtttc gatattgaaa acggtaaaaa agtgcagact
     1861 tatccggggc gtttcccaat gggtaagtcc gacttccggg tgcacacctt tgaagaagag
     1921 attgaatttg ttcaggggtt aaatcactct accgggaaaa atatcggtat ttatccagaa
     1981 atcaaagcgc cgtggttcca tcatcaggaa gggaaggata ttgcggcaaa aacgctggaa
     2041 gtgctgaaga aatatggtta caccggtaaa gacgataaag tttatttgca atgttttgat
     2101 gctgatgagc tgaagcgtat taagaatgag ctggaaccca aaatgggcat ggagctcaat
     2161 ctggtacagc tgattgccta taccgactgg aatgaaacgc agcagaaaca gccggatgga
     2221 agctgggtta attacaacta cgactggatg tttaagccgg gtgccatgaa acaggtggcg
     2281 gaatatgcag atggtattgg tccggattac catatgttga ttgaggagac atcgcagccg
     2341 ggtaatatca aactcactgg catggtgcaa gatgctcagc agaataaact ggtagtgcat
     2401 ccttataccg tgcggtcaga taaactgcct gaatacactc ctgatgtgaa tcagttatat
     2461 gatgctctgt ataacaaagc gggtgtaaat gggctgttta ctgatttccc tgataaggca
     2521 gtaaaatttc ttaataaaga gta
//
LOCUS       DAZKIV010000303         2446 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZKIV010000303 DAZKIV010000000
VERSION     DAZKIV010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.305"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            73..561
                     /locus_tag="ACHOT6_004847"
     CDS             73..561
                     /locus_tag="ACHOT6_004847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HFP9949895.1"
                     /translation="MKNKLLIAAVLTGLLSGCVDGNSPSGSQEGVVKADPRYSECNLP
                     DVNERGPIAAGLYISGTLPEGYWLMKPERKMHYKGDGIYQLVYSEKAGAVTMQFATMN
                     WNPQFTVTGLELPLNQAKTLKKGGMDKNTKVVIPADGKYVWTIQIGPDKKAIAAMVAP
                     CK"
     gene            644..1771
                     /locus_tag="ACHOT6_004848"
     CDS             644..1771
                     /locus_tag="ACHOT6_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFP9949896.1"
                     /translation="MAAVILRKVEKIYYNGFKAVHGIDLDINDGEFMVFVGPSGCAKS
                     TTLRMIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQNYALYPHKTVFDNMAFGLK
                     MQKRPKEEIKKIVEEVAEKLEISDLLYRKPKEMSGGQRQRVAVGRAIVRKPDVFLFDE
                     PLSNLDAKLRVSMRVKIAQLHQSLKDEGHPATMIYVTHDQIEALTLGDRICVLNQGRI
                     MQVDTPVNLYNHPANRFVAGFIGSPSMNLIDAVVREEDGKLYIDIAKNIRLYIPENKQ
                     SVLKAYIDKPVCFGIRPEHISLSVNCKEMNTVTGALSIIENMGSEKYLYFTVNNKEFI
                     AKVNDQSITTADIGKNLYFNLDTSFCHIFDFYTEENLTNYL"
     gene            2178..>2446
                     /locus_tag="ACHOT6_004849"
     CDS             2178..>2446
                     /locus_tag="ACHOT6_004849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949897.1"
                     /translation="MIHLVNKLINFGFTRTDAQVYISLLKNGRSSGYKIAKEISLSRS
                     SVYSSIDNLYKNGYIFLVDGETKEYEAKSPELILSQIEKSTIDNI"
BASE COUNT          783 a          441 c          485 g          737 t
ORIGIN      
        1 acattaacat taacattaac actgacatag aaatagctta actaagctat actactttta
       61 ttgagagttt aaatgaaaaa taaattgctt attgcggctg tgttgactgg tcttctttca
      121 ggatgcgttg atggcaattc accctctggc agtcaggaag gtgtcgtgaa agcggatcct
      181 cgctattcag aatgtaacct ccctgatgta aatgaaagag ggccaattgc tgcgggtctt
      241 tacatttccg gaaccctccc ggaaggctat tggttaatga agccagagcg taaaatgcat
      301 tataagggag atggtattta tcagcttgtt tattctgaaa aagcaggtgc ggtaacaatg
      361 caatttgcga ctatgaactg gaacccgcag ttcactgtaa caggtcttga attgccatta
      421 aatcaggcta aaacactcaa gaaaggaggc atggataaaa atacaaaagt cgtcattcct
      481 gccgatggaa agtatgtttg gacgattcaa attggtcctg ataaaaaggc aatagccgcc
      541 atggtagcac catgtaaata gttattttta gcactccact attcaacatt cgttgaatag
      601 tggagtatca gtattccaaa attcttgtta atgagagcag attatggccg cggtaattct
      661 acgcaaagtt gaaaaaattt attacaatgg attcaaagct gttcacggca ttgatctcga
      721 tataaacgat ggtgaattca tggtttttgt tggaccatct gggtgtgcca aatcaactac
      781 attacgcatg attgcaggtc ttgaagacgt atcaggtggt gaaatttaca ttagcgaccg
      841 ccttgttaat acccttccgc ctaaagatcg cagcattgca atggtgttcc agaactatgc
      901 gctgtatccc cataagactg tatttgacaa tatggctttt ggtttaaaaa tgcaaaaaag
      961 gccaaaagaa gagattaaga aaattgtcga agaagttgca gaaaaacttg aaattagcga
     1021 tttactttac cgaaaaccga aagaaatgtc gggtggtcag cgtcagcgtg tcgcggtagg
     1081 ccgtgcaatt gtccgtaagc cagatgtttt cttatttgat gagccactat ctaaccttga
     1141 tgcgaaactt cgtgtttcaa tgcgagtcaa aattgctcag ttgcatcagt ctctcaagga
     1201 tgagggtcac ccagcaacca tgatttatgt tacacacgat caaattgaag cattgaccct
     1261 cggtgatcgt atttgtgtgc ttaatcaggg gcgaataatg caggtcgaca caccagttaa
     1321 tctgtataac caccctgcta acagattcgt tgctggcttt attggttccc cgtcaatgaa
     1381 tttgatagat gcggttgtac gtgaggaaga cggtaaactc tatattgaca ttgccaaaaa
     1441 tattcggttg tacatacctg agaataaaca atcagtactc aaagcgtata tagataaacc
     1501 cgtttgtttt ggtattcgtc cagaacatat ctctttgagt gtgaattgca aagaaatgaa
     1561 tacagtcact ggtgcattaa gtattattga aaatatgggc agtgaaaaat atctttactt
     1621 taccgttaac aacaaagagt ttattgcaaa agtcaacgat caatcaatta ctacagccga
     1681 tattggtaaa aatttgtatt tcaaccttga tacaagcttc tgtcatatat ttgattttta
     1741 cactgaagaa aatctaacta attatttgtg attgatatga caataaccct aggttgaata
     1801 acggtattaa accttacaat tccataaggc ttcttactgc tcgaactgtc tattggtaaa
     1861 tgtcattaac tccacgaact gattcttatc gaggtaattg agttacaaaa ttccatcaat
     1921 aaaaaagagc atatatgaat aaaccgttga taacaccgct tactcagtga gcctactatt
     1981 taggtcagag catgtcttct gaccatagtt ggagggcatt cagattcgca gttcacaatg
     2041 cttacgtact gtagacctgt aaccagcttg gggatgaatg acgataaatc cacattttag
     2101 ctcagcaatt ttttcgaaaa tgttaattaa taatgttaaa attggtaaga tttcattttc
     2161 atttcaggag cgcgtctatg atacatttgg taaataaatt gataaatttt ggtttcactc
     2221 gcaccgatgc acaggtttac atcagtctgt tgaaaaacgg tcgtagcagt ggttacaaga
     2281 tagcaaaaga aatttcactt tctcgttcgt ctgtatactc atctattgat aatctttaca
     2341 agaacggtta tatctttctt gtcgatggtg aaactaaaga atatgaagca aaatctcctg
     2401 aactcatact ttcacaaatt gaaaaaagca ctattgacaa tataaa
//
LOCUS       DAZKIV010000304         2439 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZKIV010000304 DAZKIV010000000
VERSION     DAZKIV010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.306"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            552..779
                     /gene="ykgL"
                     /locus_tag="ACHOT6_004850"
     CDS             552..779
                     /gene="ykgL"
                     /locus_tag="ACHOT6_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
                     /protein_id="HFP9949898.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(814..954)
                     /gene="ykgO"
                     /locus_tag="ACHOT6_004851"
     CDS             complement(814..954)
                     /gene="ykgO"
                     /locus_tag="ACHOT6_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFP9949899.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(954..1217)
                     /gene="ykgM"
                     /locus_tag="ACHOT6_004852"
     CDS             complement(954..1217)
                     /gene="ykgM"
                     /locus_tag="ACHOT6_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFP9949900.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1583..1684)
                     /gene="ykgR"
                     /locus_tag="ACHOT6_004853"
     CDS             complement(1583..1684)
                     /gene="ykgR"
                     /locus_tag="ACHOT6_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFP9949901.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2158..2247)
                     /locus_tag="ACHOT6_004854"
                     /pseudo
     CDS             complement(2158..2247)
                     /locus_tag="ACHOT6_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          718 a          446 c          541 g          734 t
ORIGIN      
        1 ttgataagaa gaaaaagaca tttggtttca taaggttatt ggcaatataa cgttcaattc
       61 ttacttgcat tttatattgg tcaggaactt tgccctgaag tgttaggaat agtcttaatg
      121 cgctgtgtga tggactagta aaaaaacaag ttttaagggt aagagataga atataaaact
      181 ataatgagct gtcattgaag atgatttgcc aatcagtaag aaattttaat ggattaacat
      241 gccgcacaaa atggttatag gtgttcatta caataagtga tggaaaattg aacgggcgta
      301 tgtacgtcat ctgttcggta agcccccaag agccagatta agtgaagtta aacgaaggct
      361 gccagctgtc acaggaacga tggcctcttt gtcagggcac tgagtgcagt tatctgtgcg
      421 cgttactcta actggcactc gaacgtcggc gacggggtta ccggtggatc agactgttcc
      481 tgtggcgtaa acgtttttat gttagtacca acagatttac cacatttatc atcttaacag
      541 tttgagtgaa aatgcaggct gcgccgcaaa gggaggagac tgagtggcgt gttcagtcta
      601 aaagagagtt gatgccagca tacaggggcg aagctggtca acaagtcaac attaagataa
      661 tggaatattc ggagagaaac gtcaggcagt tggcctcaaa tgaacaggaa gaatatatcc
      721 ccaggaaaat taatgttggt gttataaaca caccaacatt aatccgatct gattattaat
      781 cagacaaata tcgttaatcc gtcgaatttt gaatcaacgt tttttcttac gaccctgaac
      841 ggccttaaaa cgtggattag atttacaaat cacatatagc cgtccttttc gcttcacaat
      901 ctgacagtct ggatggcgtt cttttgcggt acgcagagag ttaaggactt tcatcatgcc
      961 ccctttttcg tgctaacaaa acgaccaaaa cgttgggtga accgtgcaac atttccttct
     1021 gatgccactg ttctcagctt ccctgtatag aacgggtgcg atttcgaaga gacatcaatt
     1081 gttacgtatg gatacgttac gccatccagc tcaatctcac ggtctgtttt gatagtcgag
     1141 ccgattttaa agtactcatc aatactggtg tcgtggaaca ccacagtacg atactcagga
     1201 tggatattgg gcttcatcat ttttacctgt tatatgttat aacataacca taaagataca
     1261 tgctcatccg gatcatttca acacccaatc cctgtgacca ttagtggtat gtggaatttc
     1321 cgcaacgggg aagtctggca ttgttagcag ccattggtga cgctcccata aacgcctgga
     1381 tgagtgattg ataatggttt cgctgagagt atggattttt cgagaatgga ggttatctcc
     1441 actttactgg ccgatgattt atcgcagtat gattgattac ctgacagcgt gtattcctct
     1501 cccacggata taataattac ttacaaaaag ggagaggatg catattttaa atatcactga
     1561 agtgaacagt ttattgccgt tattaataga aatggagaaa taaataggcg tattctacaa
     1621 ttgcgacaaa aacaacgata ttaatcagtt tatgactgat ttgttgtact ttattctctt
     1681 tcattggtac ttcctcgctt taaaaaagag tgcacttcgt aagtgccctt atataaataa
     1741 cgagtttggt caaccaattt tttgacatgt atcacaaatt tgaatagatg tattacatcg
     1801 gctatctttt attgcaccaa cgtcattgat atatgtcgcc tgaagtcagt tccgggaatg
     1861 agtctgatct caagactggc ccagtccggg cgttgattgg tgctgaggag tatatcgcat
     1921 ctcatcataa tgtcgtatct cctggggtgt tatacaagat atcgttgttg gtaacctggg
     1981 cgaggaattg agttctatta aaccgtcaac tatgccagat acatactgga ttacactgca
     2041 ggcacgcctt acgagagaac gtgccgcagt gacgggttaa ttatctgaaa gaatttgcga
     2101 ggctgtatcg gttactcatt ggtttgatag ttttactctc gggagagtaa tagatattta
     2161 attcattaac ggaaaccagt cagttccttt cgatgcctga atttgatccc atagtttatc
     2221 cggaatggtt aattcatgaa caagttgtgt tatcgttcat gagaagcata acgtaaaggg
     2281 aaaagctcga ttagacggca ggatttgtca ggggttatga acgaaattca taaatctgtt
     2341 tgagtgttgc gatgggtagt gcaagttcga tatctccgca atttacagtc tgatgaatga
     2401 aaatgaatat ccataaaaaa tatattggtt tatcctggc
//
LOCUS       DAZKIV010000305         2412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZKIV010000305 DAZKIV010000000
VERSION     DAZKIV010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.307"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            307..501
                     /locus_tag="ACHOT6_004855"
     CDS             307..501
                     /locus_tag="ACHOT6_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949902.1"
                     /translation="MPENNTRKPDKSATVHIDAGTMEKIERYQQFIKDNHPGMPVPTK
                     GQITRSAVEYWYRATLGAWL"
     gene            498..2387
                     /locus_tag="ACHOT6_004856"
     CDS             498..2387
                     /locus_tag="ACHOT6_004856"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFP9949903.1"
                     /translation="MKTWFSIKAMADVVYVRIYDEIGGYGVKASALTDEINACGNASE
                     IHLRIHSPGGDIFEGLAIYNALKNHPAKKIVHIEGMAASMASFIAMCGDHIVMPENAM
                     MMIHAPRGVTAGVSGDVRRFADLMDKLGDTMAETYAGRTGRSKQEITAMMEAETWMDG
                     NECKANGFADEVIPAITAMARIESKRIGDFSNMPEKIKSMISQKTTSGEQERLNGIRE
                     LFGTFNGRYNDLAISCLADSECSVENARERLLLAMGKESTPTNKTTPANLYYAYTDNG
                     NITGDAMRQGLNARLGHERAERGNPYAMMSLFDMAQASLTHRGISTGSYGTRSQIVNA
                     AFTHSSSDFTDILAGGAEKSVLAGWEHSGETFRQWTKKGSLSNFREARRVGMNGFSTL
                     NKVPEGAEYKYITTSDRGEPIALATYGNIFSITRQAIINDDLDQLSTVPMAMGRAASR
                     TVGNLVNLVLTGNVKLSDGIALFDKKHSNLIEAGLTTPGLSAARHLMRTQKDKNGEVL
                     NIAPKFLLVPAALEDRALQMINSTAPFGADKNSGIFNPYHKLLDIIVDPRLDDISEKQ
                     WYMLSAQGTDTIEVAYLDGNDEPYLEQQEGFIVDGVAWKVRIDAGVAALDYRGMVKSG
                     GTDSL"
BASE COUNT          699 a          562 c          608 g          543 t
ORIGIN      
        1 atttttagtg tcgaatcagg ttgttttttg ttcatcactg gaacgttccc gaaaacattt
       61 acaaaaaaca ggcgcaaaaa aagcgccccc gattgctgtt accggaggcg cttttacacg
      121 acaaaggagt ttttatcgcc aggatgacga gtcttaatac tgcttcaatg gcaaaaatgc
      181 gtcaataact ttgcctttct gagaataatc agaaaaatca taatctgatt ttcaggtaga
      241 aaatgattta tctattactt ttatcgatca ataatgatgc ccgttaatca aaacggaggc
      301 ggatttatgc cagagaacaa caccagaaag ccggataaaa gtgccacggt acacatagac
      361 gccggaacta tggagaagat cgaacgctat cagcagttca tcaaagataa tcacccgggt
      421 atgccagtac ccacgaaagg acaaatcaca cgcagcgcgg ttgaatactg gtacagggca
      481 acgttagggg cctggctatg aaaacatggt tttccattaa ggctatggca gatgttgtct
      541 atgtgcgcat ttatgacgag atcggcgggt acggtgtaaa agcatcggca cttactgacg
      601 agatcaacgc gtgtggtaat gcgtctgaaa tccatcttcg catccattca cctggtggcg
      661 acatctttga agggctggct atctataacg ccctgaaaaa tcatccggca aagaaaattg
      721 tacacattga gggcatggcg gcttctatgg cctcgtttat tgccatgtgt ggcgatcaca
      781 tcgttatgcc tgaaaacgcg atgatgatga tacatgcccc ccgtggtgtt actgccggag
      841 tgtcgggcga cgttcgccgc tttgctgacc tgatggacaa gctgggcgac acgatggcgg
      901 aaacctacgc cggaagaacg ggtaggagca aacaggaaat caccgccatg atggaggcgg
      961 aaacctggat ggatggcaat gagtgtaagg ctaacggctt cgcagatgag gttatacccg
     1021 cgattacagc aatggcccga attgaatcaa aacgaatcgg agatttttca aatatgccgg
     1081 aaaaaattaa aagcatgatc agccagaaaa ctaccagtgg cgaacaggaa cgacttaacg
     1141 gcatccgtga attgtttggc acgttcaacg gaagatataa cgacctggct ataagttgtc
     1201 ttgctgattc agaatgtagc gttgagaatg cacgcgaacg ccttttactc gctatgggta
     1261 aagaatcaac gccaacaaac aaaaccaccc ccgcaaatct ttactacgcg tacacggata
     1321 acggcaacat aaccggcgat gcaatgcgcc aggggcttaa tgcgcgtctt ggtcacgaac
     1381 gggccgaacg cggtaatcct tacgccatga tgagcctttt cgatatggca caggcatcat
     1441 taacccatcg tggtataagc acgggcagct acggcacacg ctcgcagata gtaaacgcgg
     1501 cattcaccca cagcagcagc gattttaccg atattcttgc tggtggcgct gaaaaatcag
     1561 tgcttgcagg ctgggagcac agcggcgaaa cattccgcca gtggacgaaa aaaggttccc
     1621 tttcaaactt ccgggaagcc cgccgcgttg gtatgaatgg cttctcaacg ttaaacaaag
     1681 tgccggaagg ggcagaatat aaatacatca ccaccagcga tcgcggtgaa cccatcgcgc
     1741 tggctactta cgggaatatt ttcagcatta cccgccaggc gataatcaat gatgaccttg
     1801 atcagttatc aacggtgcca atggctatgg gccgtgcagc atcaagaacg gtgggaaatc
     1861 tggttaatct ggtgcttaca ggcaacgtaa aactttctga cggaatagcg ctgtttgaca
     1921 aaaaacacag caacctgatt gaagcaggac tgacaacacc gggacttagt gcagcacgtc
     1981 acctgatgcg cacacagaag gacaaaaatg gcgaagtgct gaatattgcg cctaaattcc
     2041 ttttagttcc ggcagcactg gaagatcgcg cgttgcagat gattaactca accgcacctt
     2101 tcggggctga taaaaacagc gggatcttta acccgtatca caagctactt gatatcatcg
     2161 tcgatccccg ccttgatgat atcagcgaaa aacaatggta catgctttcc gcacagggaa
     2221 cggacacaat cgaggtggct tatcttgatg gcaatgacga gccttacctt gaacagcagg
     2281 aaggttttat cgttgacggc gtggcctgga aagtccgtat tgatgcaggt gtggcagctc
     2341 tggattatcg cggtatggtc aaatcaggcg ggacagattc actatgacaa caaggcggca
     2401 ccagccgcct tt
//
LOCUS       DAZKIV010000306         2361 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZKIV010000306 DAZKIV010000000
VERSION     DAZKIV010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.308"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1793)
                     /locus_tag="ACHOT6_004857"
     CDS             complement(<1..1793)
                     /locus_tag="ACHOT6_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HFP9949904.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHSYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKTLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKT"
     gene            complement(1882..>2361)
                     /locus_tag="ACHOT6_004858"
     CDS             complement(1882..>2361)
                     /locus_tag="ACHOT6_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator
                     sugar-binding domain-containing protein"
                     /protein_id="HFP9949905.1"
                     /translation="VSGFDNLHQKAKEMLSSAECEIYLNTDFDLHFLKNEICEALNRH
                     VRVIVFSFNKLITPDPRVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLY
                     SNNRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKSR"
BASE COUNT          756 a          450 c          419 g          736 t
ORIGIN      
        1 gtcttgttta aataaaaaca gccttcaatg tattgtttgc cctctggtga ggttgaatat
       61 gaaaaagatt ccatatcagc ataagagaac tggatagatt catcagctct actacagggt
      121 atgtatcctg agtcaaaata tttcccgttc agcagagttt ttttgattaa aatatcgctc
      181 tcaagctcaa ttgcatataa aacgggacct ctggaaatat aagtgtcacc acataagtca
      241 gtattaaact gtatatcagc tataaattta atgtcaatca catcatgatg ttgccatgtt
      301 ctatctaaaa taataagtga gttttgagta tcatttttag taaaagtaac accattaata
      361 atcatcattt ttgcccagcc ggggtaacgg aaactgagag caaaacgaac agatgactca
      421 ggctcaatga gacattttac gcttaaatca tgagggtatt cagttaattg tgtaatttta
      481 attgcaacat tattaaatgt gccataaaac tcacacgggc cataaaataa tgcagtaaaa
      541 ccattgttat tttcttgaaa catattttcg acaaaagccg gtatggttct tgctgtattc
      601 ggtacgcagc agacagcagc ctcctcatga gtaggggagt atttatatct ggtattaaac
      661 tgattactgt ccggatgttt cctttcattt gcttcataac aattatcagt cttacagtac
      721 attatcgcgt gaagtatggg atgctgcatg ccaaaactag cattatagaa tagccactca
      781 gcatcatcgg ccagaccaat attagtggtc agttttatta acataacata agtgctaaat
      841 agttcatgaa cagaacaata ttcatagcct gtataagtgg cattagctgt gcgtcctgta
      901 atccattcat caccaatggg agcgccagat ggtgtaagat aatatttcaa ctgggataaa
      961 aaaatatcta atgcagtttg atactgcggc cgttggctcg ctgcgatggc taatgcccta
     1021 atatgctcat acgaatgtac gccatgacct ttaagaaggt aaccagggtc tatgacattt
     1081 ttatattgca gatcttgctc cgacacgcat cctgcggaat aatcttcata cagccacaag
     1141 caatatgaga gatattcaat gttattggtt atttcataaa ggcggtagaa aacatctgtt
     1201 attgttaatc cgtggctatg tccagcaaaa ccctgttcca caacgaaagg atgtgacgtt
     1261 tctttagggt agccagccat aatgcattgt gcggcttggg taagagaagt taataaactc
     1321 tcattacggg ttgactcata acatccaagc acaaaacgca aggcagtagc ttgtgcccag
     1381 aactctccgt tttctgcttt acagttaaat tttaatgatg aatcataaat tccaatgtag
     1441 ccatcatcat cttgcgtatc gataagttcc aaacataaat cgctcgcttt ttcctgccat
     1501 tctttattat ctaacaagta ggcactgcgg caatatccat ccttccaatt cgattgagtt
     1561 tcactatccc accaataata ttgcatcatg ttatcatcgg gattattctg ttttttaata
     1621 acgccatctt cagcagatac agtgcgtgga gttatacgat ctcttccata tattttatgc
     1681 tccgtaataa gatggggaac taatttatcc agttctccta tatgaccgtt catataccat
     1741 tccatcttat tctttaacca accagtgggc tttatatcgt taaaaaaaat cataaataca
     1801 cctttgcata aaaaagttta gacgttataa aagacaaata gattgtgatc gcctacagac
     1861 gatcacaatg aaaggaaaat attaacggct tttgctatca ctcaaatgtt tttcatcctg
     1921 caccatatca ttgccagatt catgcaaagt atttattaat gcattggcat ctggatattg
     1981 ttgctgatac ttggttagat atatatcact atggatatgt tcggcaatta tattgataag
     2041 caagcgatta ttcgagtata accccagtga ttcagctcgt tgtgaaaaaa ccattactgt
     2101 cttcatatca acaacaagca taaaacggtg agaagggtaa tattcttctt catggttaaa
     2161 gcggtaatac agttctacac gtgggtcggg agtaataagt ttattaaatg aaaaaacaat
     2221 aactcgaaca tgtcgattca gagcctcaca aatttcattt ttcaaaaaat gtaagtcaaa
     2281 atccgtgttg aggtaaattt cacactctgc agaagaaagc atctctttag ctttctgatg
     2341 taagttatcg aaaccagaga c
//
LOCUS       DAZKIV010000307         2343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZKIV010000307 DAZKIV010000000
VERSION     DAZKIV010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.309"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            222..428
                     /locus_tag="ACHOT6_004859"
     CDS             222..428
                     /locus_tag="ACHOT6_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9949906.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKVARGE"
     gene            complement(684..956)
                     /locus_tag="ACHOT6_004860"
     CDS             complement(684..956)
                     /locus_tag="ACHOT6_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949907.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            1116..1649
                     /locus_tag="ACHOT6_004861"
     CDS             1116..1649
                     /locus_tag="ACHOT6_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9949908.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            1870..1983
                     /locus_tag="ACHOT6_004862"
     CDS             1870..1983
                     /locus_tag="ACHOT6_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949909.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            1985..2281
                     /locus_tag="ACHOT6_004863"
                     /pseudo
     CDS             1985..2281
                     /locus_tag="ACHOT6_004863"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            2238..>2343
                     /locus_tag="ACHOT6_004864"
     CDS             2238..>2343
                     /locus_tag="ACHOT6_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFP9949910.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPAPD"
BASE COUNT          595 a          478 c          645 g          625 t
ORIGIN      
        1 ctggcggtat attttctgac agacagtgac gggtgttgtc aagatattgt gtcatttata
       61 acctgaatca ggggggggcc ggaatgttat ctggcatttt tagcagagcc tgaatgccat
      121 aatcacggct cccggagttg gccgtcagtg ggtgacactg gcggcttttt tgtttttctt
      181 tactttcatt ttctgtcggc ggtgacggag acatacatca gatggaaaaa atcacaacag
      241 gtgtgtcata caccacgtca gcggtgggga cgggatactg gttactgcag ctgctggaca
      301 aagtctctcc gtcccagtgg gtggcaatag gtgtgctggg aagtctgctg tttggcctgc
      361 tgacgtatct gactaacctg tatttcaaaa tcagagagga ccgtcgtaag gtggcgcggg
      421 gagagtagtc gatgaataaa caatacgaac tggttgtaaa atgaatattt ctaactgaaa
      481 aaacgttcca tgaggtaaga aaaggtcaca ggcaatcaat aacaggacgt gatgaaagac
      541 ccttgcattt gtgcgctttc tctttagata gcagcagata ctgaaaatct gagttgtcgg
      601 ggagtcaggg atacagctgt gcaagagttg gtcattgtga ttccattgaa atcctgtatg
      661 ccatgaaggg caggatttta tggctacctg agctttggtg atagtaagtt gaaaattcgc
      721 attttttgct gacatgcgta acgagaatcc cataaacagg gaggacttaa ttcttcatta
      781 acccatgcgt tgatattatg tttcagccgt tgaagcatca gcggtgttaa tgttgtggta
      841 ataatatcca gcgttttatg tgagatctta ccgtaagggt ctgcaagaat gctgcttgtt
      901 gcttcgttat tatctgccat cagaagaagt aactctgatt taacgttttc tgtcattagt
      961 tgtaaaaatc ttctgcgcaa actttcttta ctgttcattt atatggcttc atttgttgta
     1021 atctgctgcg tctcaaggga tatgtttatg agagcgacca tgagtgtttg attatatacc
     1081 taacatatca agggattaga aatcgataaa tccccatgaa cgaaaaaata aaatacggcc
     1141 tgtcggctgc cgttctggcg ctgattggtg caggtgcttc tgcgcctgaa atcctcgacc
     1201 agtttctgga tgaaaaggaa ggtaaccaca ccacggcata ccgtgatggt gcggggatct
     1261 ggaccatctg ccgtggtgcc attctggtgg atggtaagcc tgttattcct ggcatgaagc
     1321 tgtcaaagga aaaatgcgac cgggttaatg ccatcgaacg tgacaaggcg ctggcatggg
     1381 tggagaaaaa catccgggtg ccgctgaccg aaccccagaa agcggggatc gcgtcattct
     1441 gtccgtacaa cattggcccc ggtaagtgct tcccgtcgac gttttataaa cgaattaatg
     1501 caggcgatcg aaaaggtgcc tgtgaggcga ttcgctggtg gattaaggac ggtggcagag
     1561 actgccgtat ccgttcaaat aattgctacg gtcaggtctc acggcgtgac caggagagcg
     1621 cgctggcgtg ctgggacatc gacagatagc agaatatttt cctgaaaaat gacgttggcc
     1681 aacgcgggtg gataacacga aatcctgaaa actggtaaaa cctaagtgaa taaaagtaaa
     1741 aaccccgttt gttggcagca agcggggttt tgtgttttct gaccttgagt aaggcaaggg
     1801 agaaattatg ggtagggagg tactttccct gtgaggaagt ataaaagatt ctttctgagg
     1861 ttgtccatta tgaaaggcat tgaagtggag acgccagcca gtctggattt aacaagagcg
     1921 gcagcttttg ccattcgtat tgtggccatt gctgttctgg tctgggcaat ccgttggtgg
     1981 tgatatgaac cgtgttctgt gcgtggttat cattgtcctg ctggtggcct gtggtgcgct
     2041 tagtctgggg ctgaatcatt accgtgatca cgccatcatc tacaaagagc agcgcgataa
     2101 aaaagccagt gagctggagc tggcgaacgc gacaattact gatatgcaga tacgccagcg
     2161 tgatgtcgct gcacttgatg ccagatactc gagggaatta gccgatgcga gagctgaaaa
     2221 tgaaactctg cgtgctgatg ttgccgctgg tcgtaagcgc ctgcggatca acgccacctg
     2281 ctccggtacc gtgcgtgaag ccaccggcac ctccggcgtg gataatgcaa ccggcccccg
     2341 act
//
LOCUS       DAZKIV010000308         2290 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZKIV010000308 DAZKIV010000000
VERSION     DAZKIV010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.310"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..1783
                     /gene="tir"
                     /locus_tag="ACHOT6_004865"
     CDS             134..1783
                     /gene="tir"
                     /locus_tag="ACHOT6_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE translocated
                     intimin receptor Tir"
                     /protein_id="HFP9949911.1"
                     /translation="MPIGNLGNNVNSNHLIPPAPPLPSQTDGAARGGTGHLISSTGAL
                     GSRSLFSPLRNSVADSVESRDIQGLPTNPSRLAAATHETCLLGGFEVLHDKGPLDILN
                     MQIGPSAFRVEAQADGTYAAIGEKNDLEVSVTLSPQELSSLQAIDTEGKNRFVFTGGR
                     GGSGHPMVTVASDIAEARAKILAKLDPGNQGGRQAKDVDTRSVGVGSASGMDDGVVSE
                     THTSTTTSSVRSDPKFWVSVGAIAAGLAGLAATGIAQALALTPEPDDPTTTDPDQAAN
                     AAESATKDLLTQEAFKNPENQKVDIDENGNAFPSGVLKDDIVAQIAEQAKEAGEVARQ
                     QAVESNAQAQQRYDDQHARRQEELQLSSGIGYGLSSALIAAGGIGAGVTIALHRRNQP
                     TEQTTTTTHTVVLQQTGGNTPAQGGTDATRAEDASLNRRDSQGSVASTHRSDSSSEVV
                     NPYAEVGGARNSLSAHQPEEHIYDEVAADPSYSVIQHFSGSGPVTGRLIGTPGRGIES
                     TYALLANSGGLRLGMGGLTSGGESAVSSITAAPTPGPARFV"
     gene            1915..>2290
                     /gene="cesT"
                     /locus_tag="ACHOT6_004866"
     CDS             1915..>2290
                     /gene="cesT"
                     /locus_tag="ACHOT6_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE chaperone CesT"
                     /protein_id="HFP9949912.1"
                     /translation="MSSRSELLLDRFAEKIGVGSISFNENRLCSFAIDEIYYISLSDA
                     NDEYMMIYGVCGKFPTDNPNFALEILNANLWFAENGGPYLCYESGAQSLLLALRFPLD
                     DATPEKLENEIEVVVKSMENLYL"
BASE COUNT          628 a          445 c          594 g          623 t
ORIGIN      
        1 gcgttatttt gcttgcatca aaaattatac tgtggtttat ttggtttatg ctcagttgtt
       61 ttatcggctg tatgctgtta cgtcatagta atataaagga acgtgtcata tttctaaata
      121 aaaggatata tgtatgccta ttggtaacct tggtaataat gtaaatagca atcatttaat
      181 tccccctgcg ccgccactac cttcacaaac agacggcgcg gcacggggag gaactggtca
      241 tctaattagc tctacaggag cattaggatc tcgttcattg ttttctcccc tgagaaattc
      301 tgtggctgat tctgtcgagt ccagagatat tcaaggactt cctacaaacc catcgaggct
      361 tgctgcagct acacatgaga catgcttgct tggaggattt gaagttctcc atgataaggg
      421 gccacttgat attctcaata tgcaaattgg accctctgca tttcgtgttg aggcgcaggc
      481 agatggtact tatgccgcta ttggagaaaa aaatgatttg gaggttagcg ttacattaag
      541 tcctcaagaa ttgagcagct tgcaagctat tgatactgag ggtaaaaaca gatttgtttt
      601 taccggggga cgtggcggta gtgggcatcc gatggtcact gtcgcatcag atatcgcgga
      661 agctcgtgcg aaaatactgg ccaaattaga cccaggcaat cagggagggc gtcaagccaa
      721 ggacgttgat acgcgttctg ttggtgttgg aagcgcttcg ggaatggatg atggcgttgt
      781 tagcgaaacc catacttcaa caacaacttc cagcgttcgt tcagatccta aattctgggt
      841 ttctgtcggc gcaattgctg ctggtttagc gggactggcg gcaactggta ttgcacaggc
      901 gttggctttg acaccggaac cggatgatcc tacaaccacc gatcctgatc aggccgcaaa
      961 tgctgcagaa agtgcaacaa aagatctgtt aacgcaagaa gcattcaaga accctgagaa
     1021 ccagaaagtt gacatcgatg agaacggaaa tgcctttccg tctggggtat taaaagatga
     1081 tattgttgcg caaatagcag aacaagctaa agaggctggt gaggtggcca gacagcaggc
     1141 tgttgaaagc aatgcacagg cgcagcagcg atatgatgat cagcatgcca gacgtcagga
     1201 ggaattacag ctttcgtcgg gtattggtta cggcctcagc agtgcattga ttgctgctgg
     1261 gggaattggt gctggtgtaa cgattgcgct ccatagacga aatcagccaa cagaacagac
     1321 gactacaaca acacatacgg ttgtgctgca gcagaccgga gggaataccc cagcacaagg
     1381 tggcactgat gccacaagag cagaagacgc ttctctgaat agacgtgatt cgcaggggag
     1441 tgttgcatcg acacacaggt cagattcctc tagcgaagtg gttaatccat atgctgaagt
     1501 tgggggggct cggaatagtc tatcggctca tcagccagaa gagcatattt atgatgaggt
     1561 cgctgcagat cctagttata gcgttattca acatttttca gggagcggcc cagttacagg
     1621 aaggttaata ggaactccag ggcgaggtat cgaaagtact tatgcgcttc tggcaaacag
     1681 cggcggattg cgtttaggta tgggaggatt aacgagtggt ggcgagagcg cagtaagttc
     1741 tataactgcc gcaccaacgc cgggacccgc acgtttcgtt taaatatatc tgtgagtatt
     1801 tagttgaggt tggggtgggg gcgttttact agcgttaatg tttcagagaa caacgttgca
     1861 gcatgggtaa ctcttgaaat tctgttatta taaatccaat taagagaaat tataatgtca
     1921 tcaagatctg aacttttatt agataggttt gcagaaaaaa ttggtgttgg atctatttcc
     1981 tttaatgaaa atagattgtg ttcttttgct attgatgaaa tttattatat ttcgttatct
     2041 gatgccaatg acgaatatat gatgatttat ggcgtctgtg gaaaattccc gacagataac
     2101 cctaacttcg ctcttgagat tttgaatgca aacttatggt ttgcagagaa tggtgggcca
     2161 tacctgtgtt atgagtcagg agcacaatcg ctgttgttag cgttacgttt cccgctcgat
     2221 gatgctaccc ctgaaaaact tgagaatgaa atagaagtcg tcgttaagtc gatggaaaac
     2281 ctgtatttgg
//
LOCUS       DAZKIV010000309         2286 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZKIV010000309 DAZKIV010000000
VERSION     DAZKIV010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.311"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..929
                     /locus_tag="ACHOT6_004867"
                     /pseudo
     CDS             1..929
                     /locus_tag="ACHOT6_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            856..1338
                     /locus_tag="ACHOT6_004868"
     CDS             856..1338
                     /locus_tag="ACHOT6_004868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9949913.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            1382..>2286
                     /locus_tag="ACHOT6_004869"
     CDS             1382..>2286
                     /locus_tag="ACHOT6_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949914.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNSHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNASKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPP"
BASE COUNT          700 a          486 c          529 g          571 t
ORIGIN      
        1 accccagctg aagagtaaca ctgagaacgc cgcacagacc acggtcagaa cgctgattta
       61 tgtcccggaa acacaggcat cttccacacc agaagcggta gtgcctcccc tgcccggcct
      121 acctgagatg aaagtggaag tgcgcagtct gacagagaat cccccacagg ccagtgtgat
      181 aaagcaaggc agtacggtaa gagggtttat cgcagggatc gcctgttcag tggctgtcgc
      241 ctcagcattg tggtggtggc aggtctatcc ggtgcagcag caactgttac aggttaacga
      301 caccgctcag ggcgcagcaa cggtgtggat ggcctcacct gaactcgaaa actatgagcg
      361 caggctgcaa caacttcttg atacctcccc ggtacagccg ctggaaaccg ggatgcagat
      421 gatgcgtgtt gccgacagtc gctggccgga aagcctgcaa cagcaacagg cctcgacaca
      481 atggaatgag gcactcaaaa cccgcgcaca gagtagcccg cagttgcgtg gctggttgca
      541 gacccgccag gacttacatg cttttgcaga tctagtgatg cagcgcgaga aagagggact
      601 aaccctttcc tatatcaaaa atgtcatctg gcaggcggag cggggactgg ggcaggaaac
      661 acccgttgag tctctgttga cgcagtacca tgatgcccgt gcgcagaagc agaatacaga
      721 tgcgctggaa aaacaaatta atgagcgact cgaaggcgtg ttaagccgct ggctgctgct
      781 gaagaataac gtcatgccag aggcggcaac cggtaccacg gccgaaaaat aattatttaa
      841 ttaaaggaga ttatcatggc aaatataagt tatttatctt tgagtggaga gacacaaggg
      901 ctaatttcag caggttgctc aacgcttgat tctgtcggaa acaaagcgca gccagaacat
      961 aaagatcaga tcatggttta tgcgttaatg cattccatct caagaagtca aaatgtcaat
     1021 catcatgaat taattatcac caaaccggtt gataaatcct caccattgct ggcaaaagca
     1081 cttagtgaca atgaaaaaat ggcaatatgt gaatttatac tctaccgaac cagtaaagcg
     1141 ggtatttacc agccttatta taaaataaat ctgtcaaaag cccgaatatc aagtattgat
     1201 tttgtcacac cgcatgctgt cctggaaaaa gaactggaac cacaggagag aattgccttt
     1261 atttatgaag atatttcctg ggagcatacg ttagccggta cgaatgcaat gagtaaatgg
     1321 caggatcgtg tacagtaact tactgtaata acataataaa ttactattgc ggagagataa
     1381 aatggctggt ttaaggaata acaacaacac acagaatgca cagtgggctg attatgtggg
     1441 tgacattctt cggggggctc agccgataaa ccagctagta cctcagcacc cttatcttaa
     1501 cgacgtccca ctcattgatg agttaaggca tcagaactct catcatgtta ttctcttgac
     1561 acttgatgtt gcaaaaaaaa tactcagccc aataacatca tttgattaca ttcattttat
     1621 cactacacat ccttcaggta taaaagatac actcgcctgg ttagttaacg cgagtaaatt
     1681 aatgactgag tttgatgaca atgggaaaat tatatttaac cttaatgcct taaagtatac
     1741 aaaagcaagt tactttgaaa tacttggaga gaaatatata aaaatcacaa catccagccc
     1801 ctggctttta gaaaagctcg gtaaatatat tttctcttca cgagcgcctc aagttctgga
     1861 actcgctatt ggctggcgag gagctttatc tgaatcaata aaaggagtta agttctgtat
     1921 ctggttttca gtagcctgga gaaccataga atttattatg agttcagagc gagatctggt
     1981 taattttctt ggcgattttt caatggatgt ggccaaagca gttatagccg gtggtgttgc
     2041 aaccgctatt ggaagcctgg cttcttttgc ctgtgttagc tttggctttc cagtaattct
     2101 tgtcggagga gcaattttac tgacagggat agtgtgtaca gttgttttaa atgaaatcga
     2161 tgctcaatgc catttatcag aaaaattaaa atatgcaatt agagatggac taaaacggca
     2221 acaggaactt gataaatgga aaagggaaaa catgactcca tttatgtatg ttcttaacac
     2281 tccacc
//
LOCUS       DAZKIV010000310         2250 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZKIV010000310 DAZKIV010000000
VERSION     DAZKIV010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.312"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(24..398)
                     /locus_tag="ACHOT6_004870"
     CDS             complement(24..398)
                     /locus_tag="ACHOT6_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949915.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDTISGNVRR
                     AFIGLYEEKQLSDNQDLHEKYFLELMDIINKGFGLLMKKKGIRIAPLENHFTASSINS
                     CDLKHHTSDGKVESNNKISINH"
     gene            complement(410..562)
                     /locus_tag="ACHOT6_004871"
     CDS             complement(410..562)
                     /locus_tag="ACHOT6_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001725971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9949916.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            complement(835..1551)
                     /locus_tag="ACHOT6_004872"
     CDS             complement(835..1551)
                     /locus_tag="ACHOT6_004872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFP9949917.1"
                     /translation="MSTIATSLGSNIGYMKNTISERIRNRRKDVGLTQQQVAKAIGIS
                     RVSVTKWENGSSKPDGENLYLLSKLLSKSPEWILYGKDGHDKADDLRLNQYPYISDNI
                     ARLPVLTWEQAGYWDMSCPVTEIPGIKNWVDVMTKTAENSFLLHVEGDAMTNSNGLPT
                     IPDGSTVLITPCSSNIRELVGKIILIQLEGTPNVTLKKVAIDGPNIYLLSLNPLYKPI
                     ELNGGYTIKGKVSQIHQYLD"
     gene            1601..1816
                     /locus_tag="ACHOT6_004873"
     CDS             1601..1816
                     /locus_tag="ACHOT6_004873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000471546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFP9949918.1"
                     /translation="MFKDDVLRYFKKKRLVAEALGISHVAVVRWKAVIPKLRAMELDE
                     ITNGELKYNPELYKKQDSTSNEGKNDS"
     gene            1813..2238
                     /locus_tag="ACHOT6_004874"
     CDS             1813..2238
                     /locus_tag="ACHOT6_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9949919.1"
                     /translation="MKIKHEHIRMAMNVWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
BASE COUNT          596 a          483 c          522 g          649 t
ORIGIN      
        1 actctacctc acctgtgaat aaattaatga ttaattgata ttttgttgtt tgattcaact
       61 ttcccatcgg atgtgtgatg ctttaaatca caggaattaa tactgctcgc tgtaaaatga
      121 ttttcaaggg gagctattcg aatccctttc tttttcatta acaagccaaa tcctttatta
      181 atgatgtcca ttaattccag gaagtatttt tcatgtaaat cctggttatc agagagctgc
      241 ttctcttcgt acagaccgat aaaggcacga cgcacgttac cggatatagt atcgatggtt
      301 tctttttcta cggtactcag gtcaagagtc gccagttgag agcgaaccac attcgatgcc
      361 atttcctgga atggtactgg taaatcttta aattccatcg tcaacctcat cagtcagtgt
      421 ttctggttaa ccagcgacgc gcgccagctt cagttttaaa cgttttgctt ctggtatacg
      481 tcatcgcggt aaacgtgccg tcctggttgg gaaacacgcc gtacaccaga gattcgttgt
      541 tgccaagatc gatagtatcc atgctgacct catttcccct taacgctggg gtagcggaac
      601 taaaaacctg ctgcgctgtt atacaaagtg ttcccgccgt catgttcata cgcctcgggc
      661 tggctactta ccccctgacc actgcttggt aactcgaagt attgcccggc gttctgtggg
      721 gcggggtggg ttggtatgtt gttaaggtaa caagagttac cttttgggtc aatgcaatgt
      781 tgcaaaaggt acatttgagg gcatgaaaaa cccgcaatga atgcgggttc tgactcagtc
      841 taagtattga tgtatttgtg aaactttacc tttaatggtg taaccaccat tcagttcgat
      901 gggtttgtaa agcggattca gtgacaacag atagatgttt ggtccgtcaa tcgcaacttt
      961 ttttagtgtt acgtttggcg ttccttccaa ttggattaag attatttttc ccaccagttc
     1021 tctaatgtta cttgagcatg gtgtgatcag cacggtagat ccgtcgggga tggttgggag
     1081 gccgttagag tttgtcatcg catctccctc aacatgcaat aaaaaagagt tttcagcggt
     1141 ttttgtcatg acatcaaccc aattcttaat accaggaatc tcggttactg gacaactcat
     1201 atcccaataa ccagcctgtt cccacgttaa aacgggcaac cgggcgatgt tgtcactaat
     1261 gtaagggtac tgattcagac gcagatcatc ggctttatcg tgaccgtcct ttccataaag
     1321 aatccattca ggagatttgg aaagcaattt tgacagtaga tacaaattct caccgtcagg
     1381 ttttgaagag ccattttccc attttgttac ggatacacga gatatgccga ttgctttcgc
     1441 aacctgctgt tgggttaatc caacgtcttt tcgacgattc cgaatacgtt cgctgatagt
     1501 gtttttcatg taaccaatgt tactaccaag tgatgttgct atggttgaca ttgttatgta
     1561 actattgtta cccttctgct cgaaataaca ggagagtttt atgttcaaag atgatgttct
     1621 gcgctatttc aaaaaaaagc gactagtagc tgaggctctt ggaatttcac atgtggctgt
     1681 tgtgcggtgg aaagcagtta ttcccaaact tcgcgcaatg gaactggatg aaattactaa
     1741 cggtgaattg aaatacaacc cagaacttta caagaagcag gatagcacct caaacgaagg
     1801 aaagaatgat tcatgaaaat caagcatgaa cacatccgca tggcgatgaa tgtctgggcg
     1861 catccggacg gcgaaaaagt accggctgcg aaaattacca aagcgtattt cgagctggga
     1921 atgacgttcc cggaactgta tgacgacagc catccggaag ccctagcccg taatacccag
     1981 aaaattttcc gctgggtaga gaaagacacc cctgatgcag ttgaaaaaat tcaggcgttg
     2041 ttaccagcga tcgaaaaggc aatgccacct ttgctggtgg ccagaatgcg cagccacagt
     2101 tcagcttatt ttcgggagct ggtggagacg cgggagcgac tggtgagaga cgctgatgat
     2161 tttgtagcag tggcaatcgc cggtttcaat cagatgaacc gtggtggccc ggcaggaaat
     2221 gctgtggcag ttcattgact gacaatagcc
//
LOCUS       DAZKIV010000311         2127 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZKIV010000311 DAZKIV010000000
VERSION     DAZKIV010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.313"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            260..607
                     /locus_tag="ACHOT6_004875"
                     /pseudo
     CDS             260..607
                     /locus_tag="ACHOT6_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
     gene            complement(600..1094)
                     /locus_tag="ACHOT6_004876"
     CDS             complement(600..1094)
                     /locus_tag="ACHOT6_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949920.1"
                     /translation="MSDEKGIISRITDAVSGAGGALMSAVGAVKEIQKMQIDYSVKEK
                     TYELVDKLMDAQQQQMSLNELLMISKDKIIELEEKINRASKWEEEKKNYEMYTPTVAT
                     VVYRLKKSANTGQPMHYLCAQCYESSVKSILQYEGFAPPSNHRMRCHRCNASYLFPKS
                     AFSK"
     gene            1304..1504
                     /locus_tag="ACHOT6_004877"
     CDS             1304..1504
                     /locus_tag="ACHOT6_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction Ral family protein"
                     /protein_id="HFP9949921.1"
                     /translation="MTTTIDKNQWCGQFKRCNGCKLQSKCMVKPEEMFPVMEDGKYVD
                     KWAIRTTAMIARELGKQNNKAA"
     gene            1687..2055
                     /locus_tag="ACHOT6_004878"
     CDS             1687..2055
                     /locus_tag="ACHOT6_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP9949922.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEGWPSMDGSEGIR
                     ITDIDTSGIFDSDDMTIKAA"
BASE COUNT          683 a          435 c          460 g          549 t
ORIGIN      
        1 tcactcgaaa aaataaatcc atataaaaaa catacagata accatttgcg gtgataaaat
       61 atctctagcg gtgttgacac aaataccact agcggtgata cttagcccgt cagcaggacg
      121 cactaaccac cattgaaggt gaggctctta aaaatttagc cctgaagaag ggcagcattc
      181 aaagcagaaa gctttgagta gcgcgaaatg cagctgcaag acagcaaccg tggggataag
      241 catcacggcg cgttactcaa agctaactga caggagaatc cagatggatg cacaaacacg
      301 ccgccgcgaa cgtcgcgcag agaaacaggc tcaatggaaa gcagcaaatc ccctgttggt
      361 tggggtaagc gcaaaaccag ttaaccgccc tattctctcg ctgaatcgca aaccgaaatc
      421 acgagtagaa agcgcactaa atccgataga ccttacggtg ctggctgaat accacgaaca
      481 gattgaaagc aacctgcaac gtattgagcg caagaatcag cgcacatggt acagcaagca
      541 tggcgaacgc ggcataacat gcagaggacg ccagaaaatt aaaggtaaat ctataccact
      601 tatttagaaa atgcagattt agggaacaga taggaggcgt tacacctatg gcatctcatc
      661 ctatggttag aaggtggtgc aaatccttcg tattgaagta tggatttcac agaagattca
      721 tagcattgag cgcaaagata gtgcattggc tgaccggtat ttgccgattt tttgagacga
      781 taaaccaccg tagcaacagt aggtgtatac atctcatagt ttttcttttc ctcttcccac
      841 ttagaggctc gatttatctt ttcttcaagc tcaataatct tgtccttaga aatcatcaaa
      901 agctcattaa gtgacatttg ctgctgttgg gcatccatga gcttatcgac aagttcgtat
      961 gttttttctt ttactgagta gtctatttgc attttctgga tttcctttac tgcgccaaca
     1021 gcactcatca gagcacctcc ggcaccagaa actgcatctg taatcctact tattattcct
     1081 ttttcatcag acatataaat cactctctta ctgtaggggt aagaggattt tactattttt
     1141 ctcgctgtag gggtacacga gaaccaccaa gcctgatgtg gttaaaagac aggcacaatc
     1201 tttactaccg caatccacta tttaaggtga tatatggaag aagaatttga agagttcgaa
     1261 gagcatcctc aggatgtgat ggaacaatac caggactatc cgtatgacta cgactattga
     1321 taaaaatcaa tggtgtggac aattcaagcg atgcaatgga tgcaagctgc aatcgaaatg
     1381 catggttaag cctgaagaaa tgtttcctgt aatggaagat gggaaatatg tcgataaatg
     1441 ggcaatacga acgacggcaa tgattgccag agaacttggt aaacagaaca acaaagctgc
     1501 ctgatagtgg cctttatttt tggcataaat aacagaataa acactgcact gtgtattcat
     1561 tccaacgagt gaatacacgg agcaatgtcg ctcgtaacta aacaggagcc gacttgttct
     1621 gattattgga aatcttcttt gccctccaat gtgagggcga ttttttatct gtgaggatat
     1681 gaacagatgt caaacatcaa aaaatacatc attgattacg actggaaagc atcaatagaa
     1741 attgaaatcg accatgacgt aatgacagag gaaaaacttc accagattaa taatttctgg
     1801 tcagactctg aataccgact caataaacac ggctctgtat taaatgctgt attaatcatg
     1861 ctggcgcaac atgctctgct tatagcaatt tcaagcgact taaatgcata tggtgttgtg
     1921 tgtgagttcg actggaatga tggaaatggt caggaaggat ggccttcaat ggatggtagc
     1981 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatgact
     2041 atcaaggccg cctgagtgcg gctttaccgc ataccaataa cgcttcactc gaggcgtttt
     2101 tcgttatata taaataagga gcacacc
//
LOCUS       DAZKIV010000312         2092 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZKIV010000312 DAZKIV010000000
VERSION     DAZKIV010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.314"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..438)
                     /locus_tag="ACHOT6_004879"
     CDS             complement(13..438)
                     /locus_tag="ACHOT6_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFP9949923.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(422..703)
                     /locus_tag="ACHOT6_004880"
     CDS             complement(422..703)
                     /locus_tag="ACHOT6_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFP9949924.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQMLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLTRSLIHENQA"
     gene            803..1222
                     /locus_tag="ACHOT6_004881"
     CDS             803..1222
                     /locus_tag="ACHOT6_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949925.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASNYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            1489..1641
                     /locus_tag="ACHOT6_004882"
     CDS             1489..1641
                     /locus_tag="ACHOT6_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFP9949926.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNSD"
     gene            1653..>2092
                     /locus_tag="ACHOT6_004883"
     CDS             1653..>2092
                     /locus_tag="ACHOT6_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949927.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNG"
BASE COUNT          643 a          475 c          449 g          525 t
ORIGIN      
        1 ggctattgtc agtcaatgta ctgccacagc atttcctgcc gggccaccac ggttcatctg
       61 attgaaaccg gcgattgcca ctgcgacaaa atcatcagcg tctctcacca gtcgctcccg
      121 cgtctccacc agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa
      181 aggtggcatt gccttttcga tcgctggtaa caacgcctga attttttcaa ctgcatcagg
      241 ggtgtctttc tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg
      301 gctgtcgtca tacagttcag gaaacgtcat acccaactca aaataagcct gggttattcc
      361 agctgctgga actttttcgc catcaggacg cgcccaggca ttcatcgcca tgcggatgtg
      421 ttcatgcttg attttcatga atcaagctcc tagtaagtgg ttgtgttaac gttttggtat
      481 cttccagctc gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt
      541 caccattact ggctcgctca ataaggacac ataacgatgc ccctaacatt tgacctttac
      601 tcaatgcctt tcttagataa ccgatgctag taccacactc acatgcaaac atacgctgtt
      661 catctgacga aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa
      721 cacagcaaga atacctacag gtaaacaaaa gtcaataccc ttgggttgtt taccttgcgg
      781 taatcgcatc tattatttac ctatggacaa atatgaattt agacgacaac aactcatcaa
      841 aattcgtgat gagaaatgcg atggtaaagc ggttaacgtg gccagaaaga tcggtcgcga
      901 gccttcttat gtatcaagaa tgttgtaccc agaggggaaa aaaggaaaaa aacggatcgc
      961 tgatgatatg gtggagatta tagaagagtc ctttgggtta ccccggggat ggatggatgg
     1021 tatcgtttca tcatcaacga acacagcctc caattatgaa acaagggttc taacgccacg
     1081 acaacgtatt tttttagatc tcttagacga actgccagaa agtgaagcgg ataacttatt
     1141 aaaaactctt gaagagaaaa aacagtatta caatatgatc tacgaagaaa tccgtaaaaa
     1201 gaaagcacaa aacgcatcat aactcaccaa acaaccagtc accagttaag acacatcaaa
     1261 aaaattaccc atgggtattt actttttaaa tacccatggg tatccttcct ttcataccaa
     1321 cccaccccgt cccacagaac gcagggcaat acttcgagtt accaggcagt ggtcaggggg
     1381 taagtagcca gcccgaggcg taagaacatg acggcagggt tcaactttaa taactatgca
     1441 gcaggttttt gttccgctac cccggcgtta aggggaaatg aggtcagcat ggatactatc
     1501 gatcttggca acagcgaatc tctggtatgc ggcgtgtttc ccaaccagga cggtacgttt
     1561 accgcgatga cgtataccaa aagtaaaacg tttaaaaccg aagctggcgc gcatcgctgg
     1621 ttagcaagaa actccgactg atgaggttga cgatggaatt taaagattta cctccttcaa
     1681 tccaggagat tgcagcacac acacttcgtc atcgtctgaa cgaacttgaa ttggaatcgg
     1741 taacgaaaaa agacactgat aatatggctc gtaatgtgcg cgatgcgttt accggattgt
     1801 atttctgtgc gtctataaat aaacacgact cagagagtgt ggcaaataaa attgcagaaa
     1861 cgacagcgca aaacatcaat acgaaaccaa cggaagaaga aattgatcag tttgctcatg
     1921 atgctggttt aaaaaacaag aaagaaaaat cgccatatgc ggggaacatg tttgtttatg
     1981 acaatctcat cagaattcgt ggcgaaattc cggcggaata cctggcaaga gtccatcagg
     2041 cattgcttaa aaatttggaa acagaattat ttgatggcaa cactaacggt tt
//
LOCUS       DAZKIV010000313         2077 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZKIV010000313 DAZKIV010000000
VERSION     DAZKIV010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.315"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            134..349
                     /locus_tag="ACHOT6_004884"
     CDS             134..349
                     /locus_tag="ACHOT6_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9949928.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            354..698
                     /locus_tag="ACHOT6_004885"
     CDS             354..698
                     /locus_tag="ACHOT6_004885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFP9949929.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            749..1279
                     /locus_tag="ACHOT6_004886"
                     /pseudo
     CDS             749..1279
                     /locus_tag="ACHOT6_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
     gene            1437..1619
                     /locus_tag="ACHOT6_004887"
     CDS             1437..1619
                     /locus_tag="ACHOT6_004887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949930.1"
                     /translation="MGKYKRILLRLTMKDGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            1632..1765
                     /locus_tag="ACHOT6_004888"
                     /pseudo
     CDS             1632..1765
                     /locus_tag="ACHOT6_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1773..2006
                     /locus_tag="ACHOT6_004889"
                     /pseudo
     CDS             1773..2006
                     /locus_tag="ACHOT6_004889"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system i-spanin subunit Rz"
     gene            1987..>2077
                     /locus_tag="ACHOT6_004890"
     CDS             1987..>2077
                     /locus_tag="ACHOT6_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949931.1"
                     /translation="MRELKMKLCVLMLPLVVSACASTPTVQAPC"
BASE COUNT          515 a          437 c          609 g          516 t
ORIGIN      
        1 cggaatgtta tctggcattt ttagcagagc ctgaatgcca taatcacggc tcccggcgtt
       61 ggccgtcagt gggtgacact ggcggctttt ttgttttcct ttactttcat tttctgtcgg
      121 cggtgacgga gacatacatc agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc
      181 agcggtgggg acgggatact ggttactgca gctgctggac aaagtctctc cgtcccagtg
      241 ggtggcaatc ggtgtgctgg ggagtctgct gtttggcctg ctgacgtatc tgactaacct
      301 gtatttcaaa atcagagagg accgtcgtaa ggcggcgcgg ggggagtaaa gcgatgaaga
      361 aaaaatacga actgggtgtt aaagggataa ataattaccc ggataagatt actgttactg
      421 tggcactgga aattggtggg tatccgtcac tgttgttgcc agatgtggcg attagtcttg
      481 accgtactga aggtgccacg ctggagtttt acgaagctga ggcgaaaaag caggcgaagc
      541 agtttttcat ggatgttgct gccgggttat gtgaagggga tggtccgttg ccggaaaagc
      601 gccccgtaat tttagaggcg caggatgtgt tgataaccta cagaggaaaa ctaccgggaa
      661 taattactgg ttctctgaag actccaccgc tggcctgaag acttaacata tccagggatt
      721 tgaaatcgat aaaccctgat aaatatccat gaacgcaaaa atcagatacg gcctgtcggc
      781 tgccgttctg gcgctgattg gtgcaggggc gtctgcgcct gaaatcctcg accagtttct
      841 tgacgaaaaa gaaggtaacc acaccacggc ataccgtgat ggtgcgggga tctggaccat
      901 ctgccgcggt accacccgag tggatggtaa acctgtcgtc ccgggcatga agttgtcgaa
      961 ggaaaaatgc gaccaggtta acgccattga acgtgataag gcgctggagt gggtggagcg
     1021 caatattaaa gtaccgctga ccgaacccca gaaggcgggt atcgcgtcat tttgtcctta
     1081 taacattggc cccggtaagt gtttcccgtc tacgttttac agacggatta atgcaggtga
     1141 tctaaaaggt gcctgcgaag cgattcgctg gtggattaag gacggtggca gagactgccg
     1201 tatccgctca aataattgct acggtcaggt atcccggcga gaccaggaaa gtgcactggc
     1261 gtgctgggga attgacaggc aagtagaata ttttgctgaa aatgacgttg gtcaacgcgg
     1321 gtgggtaaca cgaaatcctg cgaactggca aaagctaagt gaataaaagt aaaaccccgt
     1381 ttgttggccg caaacggggt tttgtgtttc tgaccttgag taaggcaagg gagaacatag
     1441 gaaagtataa acgaattctg ttgagattga ctatgaaaga cggccttgaa ctgaaagcgc
     1501 ctgtaactga tgacgtcagc agagcgctgg cttttgctat taagtgggtg gcggtcggta
     1561 ttgctgtgtc tccgatgctg tatgggctgg caaaactggt cattgcgttg aaatcgtgaa
     1621 gaggattaag catgtcagac aagctcataa cgctggcgaa gatcctctgt gtaatcgtcg
     1681 gcatttcatt ttcactaata tgctggttgc tatttgcttt tccactgcct ggcgcgtctt
     1741 aactttgtca gggctggtgg ggtgaggggg atatgaaccg tgttctgtgt gtggtgatta
     1801 ttgtcctgct ggtagcctgt ggtgcgcttg gtctggggct gaatcattac cgcgataacg
     1861 ccattaccta caaagcgcaa cgcgataaaa aagccagtga gctgaagctg gcgaacgtga
     1921 caattactga tatgcaggta cgccagcgtg atgtcgctgc acttgatgcc agatactcga
     1981 gggaatatgc gagagctgaa aatgaaactc tgcgtgctga tgttgccgtt ggtcgtaagc
     2041 gcctgcgcat caacgccaac tgtccaggct ccttgcg
//
LOCUS       DAZKIV010000314         2069 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZKIV010000314 DAZKIV010000000
VERSION     DAZKIV010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.316"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..1075
                     /locus_tag="ACHOT6_004891"
     CDS             38..1075
                     /locus_tag="ACHOT6_004891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFP9949932.1"
                     /translation="MVLIKKNVLSASIATVLLGSTGAFAANDRGFTSDTDWSEEQAGF
                     NGHVGTTFEYETKSTDNYLGKTVKEYTKTHEIFQAYFSQPNWQFAAIYGLKSIDRRQE
                     EKGYHENETSLQNYISLNKTFTIGNGFDFSLVNDLMYTDGNIDSPYVTGLHTVTVDNS
                     FRPTLTYFNSDWNAGFYSNVEYLYSRNDKSSWGVREEEGQSILFQPYKRFGAWEFGIE
                     FFYQKKHNDEFNHGKINDRSIYTETYYEPIIKYAFENAGNLYLRTRFGHGKTENADSL
                     QYNANRTYFKTIRKATLGYEQNIGDDWIAKIEYKHAWEKEHKNFYDPRDEEKYNVLNQ
                     DNIYVHAFYRF"
     gene            1094..2050
                     /locus_tag="ACHOT6_004892"
     CDS             1094..2050
                     /locus_tag="ACHOT6_004892"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000490374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFP9949933.1"
                     /translation="MFSALDIKTLMQGTLYGDPSLRVDTIRPIHSPLVGGLSIVMTPG
                     DLLHIPTTGADIIIGPEEIISSSAKAKIVVDYLNVNNLNKVLRYYKVHKYRLFEQENT
                     STIPDVYIGKHCQIGMNCHFMPGVKIMNCVTIGDNVAIHANTVIKEGTIIGNDVIIDS
                     NNSIGNYSFEYMADERDSYVRVDSIGRVIIGDDVEIGCNNTIDRGTLGDTIIGQGTRI
                     DNQVQIGHDCIIGNKCLIVSQCGFSGHVVLGEHVITHGQVGIAGHISIGSYSVIKAKS
                     GVSHSCPEKSDLFGYPAKNTREYNKNLAVLNKLTKQHGVYKQ"
BASE COUNT          690 a          366 c          419 g          594 t
ORIGIN      
        1 tcatatctta aattgatatt ttggaaggaa ctcatttatg gttttaataa aaaagaatgt
       61 cttatcggct tcaattgcaa cagtcctttt aggctcaaca ggagcctttg cggcaaacga
      121 tcgcggcttt acctccgaca ctgactggtc agaagaacaa gcgggcttta atggacatgt
      181 tggtactaca tttgaatatg agaccaaaag cactgataac tatttaggta aaacagtaaa
      241 ggaatatact aaaacacacg agattttcca ggcctatttc agccaaccta attggcaatt
      301 tgcagcaatc tatggcctta agtctatcga tcgccgacaa gaagaaaaag gctatcacga
      361 aaatgaaaca agtctgcaaa actatatatc actaaacaaa acgttcacta ttggtaacgg
      421 tttcgacttc agtctggtaa acgacctgat gtataccgac ggaaatatag atagccctta
      481 tgttacaggc ttgcatactg taactgttga caacagtttt cgccctacgt tgacgtactt
      541 taactctgac tggaacgcag gtttttattc aaacgttgaa tacttataca gtcgaaatga
      601 taagagctcc tggggcgtac gcgaagaaga aggtcaaagt atactatttc agccctataa
      661 acgttttggt gcatgggagt ttggtattga atttttctac cagaaaaagc ataacgatga
      721 atttaatcac ggtaaaataa atgatcgtag tatttatacc gaaacttact acgagccaat
      781 tatcaaatat gcttttgaaa acgcaggtaa tctgtatcta cgcacccgct tcggacacgg
      841 gaaaacagaa aacgcagata gtttgcaata caatgcaaac agaacctatt tcaaaacaat
      901 tcgaaaagcc actctaggtt atgaacaaaa cataggtgac gactggatag ccaagattga
      961 atataaacat gcctgggaaa aagagcacaa aaacttttat gatccaagag atgaagaaaa
     1021 atataatgtg ctcaatcagg ataatattta tgttcatgct ttctatcgct tttgattaaa
     1081 cagagggaaa cctatgttca gcgcactaga tattaaaacg ttgatgcaag gaactttata
     1141 tggtgatcca tcactcaggg ttgataccat tcggccaatc catagtcctt tagttggtgg
     1201 actctcgatt gttatgacac ctggagattt gttgcatata ccaacgactg gtgcggatat
     1261 aattatcgga ccggaagaga tcattagttc tagcgctaag gcaaagattg tagtcgatta
     1321 tctgaacgtg aataacctta acaaagtttt aagatactat aaggtacata agtatcgttt
     1381 gtttgagcaa gaaaacactt caacgatacc tgatgtatac attggtaaac actgccagat
     1441 aggtatgaac tgccatttta tgccgggtgt taaaattatg aactgtgtga ccataggtga
     1501 taatgttgcc attcatgcga atacagtgat taaagaaggc actattattg gtaatgatgt
     1561 tattattgat tctaataatt ccatcggtaa ttatagcttt gaatatatgg ctgatgaacg
     1621 tgattcatat gtccgagtcg atagcatcgg tagagttatc ataggtgatg atgttgaaat
     1681 cggttgtaac aacaccattg accgaggaac acttggagat acgataattg gtcaaggcac
     1741 tcgtattgat aatcaagtgc agattggaca tgactgcata atcggcaata aatgcctgat
     1801 tgtttcacaa tgcggtttct caggacatgt tgttttaggc gaacatgtta ttacacatgg
     1861 tcaagtcgga atcgccggtc acatatcaat tggtagttat tcggtaatta aggctaaatc
     1921 aggtgtaagc cattcttgtc ctgaaaagag tgaccttttt ggttaccctg ccaaaaatac
     1981 gcgagaatac aacaaaaatc tcgctgtcct taataaacta actaaacagc atggtgtata
     2041 taaacagtga gtcgttgaga agtattatt
//
LOCUS       DAZKIV010000315         2024 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZKIV010000315 DAZKIV010000000
VERSION     DAZKIV010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.317"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..547)
                     /locus_tag="ACHOT6_004893"
     CDS             complement(<1..547)
                     /locus_tag="ACHOT6_004893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFP9949934.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDE"
     gene            complement(563..1297)
                     /locus_tag="ACHOT6_004894"
     CDS             complement(563..1297)
                     /locus_tag="ACHOT6_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HFP9949935.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(1294..1625)
                     /gene="sctI"
                     /locus_tag="ACHOT6_004895"
                     /pseudo
     CDS             complement(1294..1625)
                     /gene="sctI"
                     /locus_tag="ACHOT6_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            complement(1645..1885)
                     /locus_tag="ACHOT6_004896"
                     /pseudo
     CDS             complement(1645..1885)
                     /locus_tag="ACHOT6_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1899..>2024)
                     /locus_tag="ACHOT6_004897"
     CDS             complement(1899..>2024)
                     /locus_tag="ACHOT6_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP9949936.1"
                     /translation="WGKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
BASE COUNT          622 a          389 c          344 g          669 t
ORIGIN      
        1 gctcatctct attttttttc gcataaaatg taattctctc taaaagagtt gatgcaagtg
       61 gtaacggcaa ttgtatgtga tcaacaaaat caggaaataa taaattaaat cgttgctgca
      121 ttgccaatgg ttgttgcctt atatagggtg acaacgttga aaaaccacat gtgatgatat
      181 tgtgatagct agcgatgccg ggtttgtatc gtgccttttc atttattttc accggtatag
      241 ctgataaata atcccttagc acagcaggaa ccttataaaa aaatccgcgt tctgcaaacc
      301 tttctcgata aaagtgataa ccactgaata aacagaacaa tggaaataaa ttccaattgt
      361 ttatgtagta aataagtgaa ctattcagat taaaatgttc aactgaaaga ttgtactgca
      421 gaacaatcat ttcattggta atagatctaa gcaccggatt gttaataggt gtattattca
      481 agcttactcg ttgaggatga atataactaa ttggatcata tataatcttt cttagggcaa
      541 gattcataac gctctcatat gtctacttaa ttttcaacct gactaaatta gttagaatgg
      601 ccctatactt ccataacagc cagcaagtcg ctacggatat taatgcaagt aagatagaaa
      661 ccggcatagc cttatcataa gcaaaaacag gttcgctaat ttcatatgtt ggtgcttgct
      721 caataatgtc tcttcgtttt gacaatacaa cagaaatatt ttcatattgt acgcttgcag
      781 agctattaac aataaatctc ttgatatcat ttatttttat ttctgggttg atatcttttt
      841 catatactgc aagtacagaa atatgaattg gtaaagcagt tttaccacta tcgccattat
      901 caacatcgta actaacatgt actctcgaag aaataatgcc atccataatt ttgagagatt
      961 gctctaaccg ctgctcaata gcagaatata gccttgcttt ttccgctcgt ggagatgaaa
     1021 cgagtgcatc tgcagggaac atctgcgata tttgaatatc aggcttaccc ggtagattgt
     1081 agatttttag ccaatccacc gcagaagcaa aatccgttgg ttcgacaaat attgaaaatc
     1141 ctgttttgcc ttgatccttc ttttcagcat taatattatg tctttgtaaa acagcaagga
     1201 catcattagc ttgctgttga tcaagatggt tcaataattc ctgctgcttg cagccgcaca
     1261 acagcaggat aaacaataat aatgatatat atttcatgag cgggtcaacg tctcaactgc
     1321 agcgacaatc tttcttgcta atgttccacc aatgaaacct cgacattata attaagcaca
     1381 tgatcctgca agactaataa tttttcagga gttaacccct ctttatcaga ccttaatgct
     1441 tgtaacgcat tttgctgcca attttcagtt tgtacagcat agttggcaaa agcggagatc
     1501 attttatcat ccgggttgtc aagattgaga acccggtcat ttaaatcaat cactttcgct
     1561 tgctgtattt catgagcagt tgattgctca gctctatcaa taggtggcat attgtttact
     1621 gacataataa aatccttatc cagtttagcg gaagttttgt atgattgctg aatcaagatc
     1681 tttatacgct tttacaacgt tagattgcgc atttcgatat aatgtatact cagctaatgc
     1741 actttgatat tcggcaagaa atttcgtgtc ggagggattg gttggctaaa tcctccaacg
     1801 ctttttcaac ttgctggttc agatcatcaa cgccctgatc aaattgattg ctgatatcca
     1861 taatataacc attccaatct gccataagtc ctcctcaatt attcctgaaa tgtccagttg
     1921 ctaccttgag acttctcgaa tcggttatct ccgtagctaa atgaactatt atttgtgtcc
     1981 tgatttttca aattcacatt aaatcttaca tattgtttac ccca
//
LOCUS       DAZKIV010000316         1978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZKIV010000316 DAZKIV010000000
VERSION     DAZKIV010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.318"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..989)
                     /locus_tag="ACHOT6_004898"
     CDS             complement(<1..989)
                     /locus_tag="ACHOT6_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9949937.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDTEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDK
                     PLYKDLIGRTKAALKKNPKNVLFAVVWMQGEFDFGGTPVNHAAQFGALVDKFRADLAD
                     MAGQCVGGSAGGVPWICGDTTYFWKQKNESSYQTVYGSYKNKTEKNIHFVPFMT"
     gene            966..1109
                     /locus_tag="ACHOT6_004899"
                     /pseudo
     CDS             966..1109
                     /locus_tag="ACHOT6_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1304..1471)
                     /locus_tag="ACHOT6_004900"
     CDS             complement(1304..1471)
                     /locus_tag="ACHOT6_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9949938.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(1468..1899)
                     /locus_tag="ACHOT6_004901"
     CDS             complement(1468..1899)
                     /locus_tag="ACHOT6_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFP9949939.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALREIEDVKHDTRE
                     AEDVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENHL"
BASE COUNT          472 a          594 c          451 g          461 t
ORIGIN      
        1 gtcatgaacg gtacgaaatg gatattcttt tccgttttgt ttttgtagct gccgtacacc
       61 gtctggtacg aggattcgtt cttctgcttc cagaaatacg tcgtatctcc acatatccag
      121 ggaacaccgc cagcagagcc accgacgcac tgacctgcca tatccgccag gtctgcacgg
      181 aatttatcaa ccagcgcacc aaactgtgct gcgtgattta ccggcgtacc gccaaaatca
      241 aattccccct gcatccacac cacggcaaac agcacatttt tcgggttctt cttcagtgct
      301 gcttttgttc gaccgataag gtccttatac agcggcttgt ccacacccca gcgggttgaa
      361 ttctccgagg caccactcgc gtcactgtat gtgccatcgg ctccggtggt gaacgctgaa
      421 ccaccacgac agcacggaac cagcagaatg cccgcattcg ccggtataaa cggcagcagt
      481 tttttggcga tatgcagccc ctgccccacg gttccgtact gcccctttga caggtccgct
      541 ttcggatggt taaggcggct catgtcctgc acatcatgca gacaatggtc cgccggaatg
      601 atgtcgttat atttgcatgc tgcaccgccc ggtgtcaccg tactgcggcg cgccagctgc
      661 ttaatacgcg ggtccggacg gtcatatgtc tccggcagcg gaaggccttc accatatgcc
      721 atgctgtttg actgccccgc cagaaccaca acaaagtaat actccgtgtc tctggtggcg
      781 ctgattactg tgccttctcc atccgacggc ttcaccaaca caggtgtggt gacatcacct
      841 tccgccgcaa tggcctgcat cagagtataa ggcgtgatgg ccaccggact accaaacggc
      901 tgccagccct ctttcagttt gtgtgtcagc ttttccgcaa ggtctgacgg cgacgccgcc
      961 ctgacaacat cataatgttt aatcgacatc gaatttctcc cgtgtacagg aacagagtta
     1021 aaaagccgga accggaatca aattacagga tggccatctg ccagtggctg gtcgtaaaaa
     1081 aaaggccacg ccatgcgcag ccggaaataa agggataacg atgatagttt gagaaaaaca
     1141 gaaacaacac ttttgtggca aagcatggtg ccgggtgcct cccggtgaat tcagtatcag
     1201 cacctgaatc cgcgattatc ccatatacct ggttgctgat cgcccctccg cacaggggga
     1261 ttcaccatgc agaagtgttt ttaataaaca gcaaacaaaa aaatcaagca ttatgcaggc
     1321 tgtttctttt tatcaccggc cacagcaata ccacaatgcc gcagaccagc accccatccg
     1381 ccagcaccga catgattctg ctggtgaaat ccaccatcac caccagaaac agcaggagtg
     1441 cagccacagc caggcgcagt tttaccgtca caggtgattc tccagacgaa gacccagaac
     1501 accggcaatc tcttccagca ccttgcgctc ttccggctca atttcgccgt ctgcctccgc
     1561 aatggccacc gccacatcca gcacatcttc cgcttcacgc gtatcgtgtt tcacatcctc
     1621 aatctcgcgt aacgcggcac gacgaccaat tttaaaattg gtatccagct gaccgataat
     1681 agttgcgcta atcgcattaa tttccgaggt aaacgcagac aacgcaggct gattacgtaa
     1741 gacctgttcg atcttcgctt tctcggatgc ctcgcattca ccatctgcat aggccaccag
     1801 gtatgcagcg ttaatcaccg cctgtgccag atcgcgtttc tcaaactttt taatttccgc
     1861 tgccgctctg cgggttttct ttttgaagat tccaaacatc gtgacgttcc tttgggtggg
     1921 tgagccaacg cccgggagcg atctgcccac agagaaagtc acactgacca ctccgtaa
//
LOCUS       DAZKIV010000317         1960 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZKIV010000317 DAZKIV010000000
VERSION     DAZKIV010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.319"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1521)
                     /locus_tag="ACHOT6_004902"
     CDS             complement(<1..1521)
                     /locus_tag="ACHOT6_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9949940.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLILTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALE"
     gene            complement(1571..1873)
                     /locus_tag="ACHOT6_004903"
                     /pseudo
     CDS             complement(1571..1873)
                     /locus_tag="ACHOT6_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
BASE COUNT          391 a          646 c          456 g          467 t
ORIGIN      
        1 ttcaagggca agcagagcct ctcttgcctg ctcctcttcc ctctccccgg cagagcgcgt
       61 tttcggtgat gtatgctttt tacctgtcag ctcttcagcc agacggctga cggcttcctg
      121 ctgcccagga cctttgctga cgcctgttgc acgcgagcgg ttgatgtacc ccatttcccc
      181 ctggcgtata cgcgcatccc gttccgcaat ggattttctc agcgccagtt catcgcgttt
      241 tgttttctca ataaatacgc ggttctcttc tgccagttca ccaaacaacg caccaacgcc
      301 gggcacattc tttgtcgttt cccaggctga ctgaataaat tcagccagcg ccagatcccc
      361 ctgcacaagc agcagcttca cttgttcaac agttccggcc accacgtcag tgatcagact
      421 gagtgccccc agtgtatgat cacctatcca tgcccatgcg tcagaagtcc aggttttaac
      481 atcgtcccag attttttcca ccggcgtggc cgctttatca agttgctcca gacgtgcatt
      541 catgacatcc gcaaacaggg acatcgcctc cgtcaccgca gcctgtttac ctttcgtgcg
      601 ctcaagctca tcaatatggc gtaactggga aacgctcagg aagttatact gctgattcag
      661 ggaggccagc gccttcaccg gatctgctgc aatcccttca aaggcttttt ccaccttccc
      721 ggcatcgtcc cccaccgtct gcagccatct ctgagaggtt tcccccatga tccgtagctg
      781 cccggcggta tatttcccgc tttctgccag acgggccaga ttttctgccg cctgtctgat
      841 accaccaccg gcttcatcgc tgatcacccc ggccattttc cacaattctg ccgttgtggt
      901 ggcagccgcc cctccggtca ggatcagtga acgcaataag gcccggtcag cctgctctgc
      961 ctgccaggcg gcggcagcaa gcgcggccag tacggcaacc ccgccacctg ccgccacacg
     1021 ggccaccgac ataaatcgtc ccagctcacc ggcattccgg gcattttcag ccagtgcatt
     1081 tgccgtatct gacagcgact cctctgatga ttcagaggca tccctgatcc cgagaagttc
     1141 ctccttcagc agggtaagca ggctgagcgg tccaccgaat gaatcgctga tctgcccccc
     1201 ctgctgcagc atgataagga agggattctg accaccggca agctgagtga caatatccgt
     1261 gaactgtgcg ggcagtgtgc gcatggcagc cttatactgt ccgactgata tcccggcttt
     1321 ttgtgccgcc agcgcctgtc ggctcagact ctgctcagca gcatcagcct gttttctgaa
     1381 agactgactg actttcccgg acatcaaatc agcaagcgca ctggtttcac ccagctcttt
     1441 ttttacccgc gctgcctctt cagaaaaacg ggcagaatcc agtgtcagca cggctgtcag
     1501 atcggcaaaa ttacctgcca tcgtggtcac ctcctgaaat gtcctctgat gccatcaata
     1561 actgtcacaa cctccttccc tcctccccga aacggactcc accggcgagg cccgccgcct
     1621 tctgcatcag tacatcattt tcgtccggcg tctccgcctg cccttcctca gcctccggag
     1681 cgaacaggct gaaatccgcc ggatgcatat ccggatcgca aaaaaacagg ctgagtacgg
     1741 cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc ctgaaaataa tgcgtgcagt
     1801 aaaaacgtcg ccagtcggca tattcggtgg atgtcatccc ggcaagcatg gcgcgccagt
     1861 cgggtctccc catctcacgc gccagtttca ggacaaagtt cagctcacct tcgaatgctt
     1921 ttttgatgtt accggctctg tcgcttctgc tttcccggtt
//
LOCUS       DAZKIV010000318         1896 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZKIV010000318 DAZKIV010000000
VERSION     DAZKIV010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.320"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(35..355)
                     /locus_tag="ACHOT6_004904"
     CDS             complement(35..355)
                     /locus_tag="ACHOT6_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9949941.1"
                     /translation="MAEKQVKDYDKFNLRFPDGMRDAIAERAKRNGRSMNSEIVQILE
                     DALNAENTLGEIADKINSVSVPLNVDALVQLQAQVIAMQKEIQEKFREQNEKLRELLN
                     KKPT"
     gene            463..636
                     /locus_tag="ACHOT6_004905"
     CDS             463..636
                     /locus_tag="ACHOT6_004905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004177050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HFP9949942.1"
                     /translation="MQDTLFTERKNIKLNLRLPSRLNEDLRRLAEMDCISLNSAIVRL
                     LAKGVREEVANGR"
     gene            707..1630
                     /locus_tag="ACHOT6_004906"
     CDS             707..1630
                     /locus_tag="ACHOT6_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001428824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9949943.1"
                     /translation="MTSLAIADRTINVPFHGTNLFLVGINNEPYVPMKPVVEGMGMVW
                     AAQFVKLKQRFVKGISEIEIPSAGGKQLMTCLAFRKFAAWLSSIQPNKVRPEIRDKVI
                     QYQEECDDVLYEYWTKGHVINPRKAKKALPGKITTEQQEAIKQLVMSRGQSLPKEKQA
                     KAMITMWSSLKSHFGCSYKEISEGQFTEALSLAARVPLEGELIGKQEKKTNELSAKEA
                     NSLVWLWDYANRSQALFRELYPALKQIQSNYSCRCYDYGHEFSYVIGMARDVLINHTR
                     DIDINEPDGPTNLSAWLRLKNKELPPSLHHY"
     gene            1685..>1896
                     /locus_tag="ACHOT6_004907"
     CDS             1685..>1896
                     /locus_tag="ACHOT6_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9949944.1"
                     /translation="MLYSNILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPEAY
                     FRIAPEDWLRAEMQGEIVALVHSHPGG"
BASE COUNT          517 a          417 c          445 g          517 t
ORIGIN      
        1 aataaacttt cgcagtaatg gattatgccc accgtcaggt gggtttttta tttagtagtt
       61 ctctcaactt ttcgttctgc tctctgaact tttcctgtat ttctttttgc atggcgataa
      121 cctgggcttg aagttgaact agcgcatcaa catttagcgg aaccgagacg ctgttaattt
      181 tatctgctat ttccccgagt gtattttctg cattcaaggc atcttccagt atctgaacaa
      241 tctctgagtt catagagcgc ccgtttcgtt tggctcgttc agctatagca tctcgcattc
      301 cgtcaggaaa acggaggttg aacttgtcgt agtcttttac ttgtttttcg gccattgcaa
      361 atctctcaaa aaaaatcatg gtgccatatt gccatacgat ttcaatagtg gcattatggc
      421 cttcatggtg tcactttggc accaaataaa ggagattaga taatgcaaga tacacttttc
      481 actgagcgca aaaatatcaa actcaacctt cgccttccat cacggctgaa tgaagacctt
      541 cgccgcctgg cggaaatgga ctgtatatct ctgaactctg caattgttcg tttgctggca
      601 aaaggtgtta gggaagaggt ggcgaatggt cgctaaaaac agcgaagcct caatggctgc
      661 aaccattgag gcttctaaat taccagttaa ccacgagaaa actgatatga ctagtttagc
      721 aattgcagat cgcacaatca atgttccatt ccacggaaca aatctctttt tggttggaat
      781 taacaatgag ccttatgttc ctatgaagcc tgttgttgaa ggtatgggga tggtttgggc
      841 tgctcaattt gttaagttaa aacagaggtt tgtcaaaggt atttcggaaa tcgaaatacc
      901 ttctgctggc ggtaaacagt taatgacatg tcttgccttt cgtaaatttg cggcttggct
      961 ttcaagcatt caaccaaaca aagtccgccc tgaaatccgc gacaaggtaa tccagtatca
     1021 ggaagagtgt gacgatgtgc tctacgagta ctggactaaa ggccatgtga ttaacccgcg
     1081 caaagctaaa aaggcattgc cggggaaaat caccactgaa cagcaggaag ccattaaaca
     1141 actcgtcatg agtcgcggtc agtctctgcc aaaagaaaaa caggcgaagg cgatgatcac
     1201 catgtggtcg tcactgaaat ctcattttgg gtgttcatac aaagaaatca gcgaggggca
     1261 gtttaccgaa gctctgtcac ttgctgctcg cgttccgctt gaaggagagt taatcggcaa
     1321 acaagagaag aaaaccaacg agctttctgc aaaagaagca aacagccttg tatggttatg
     1381 ggattatgct aaccgctcac aggcattatt ccgcgaactg tatccggcgc taaagcaaat
     1441 tcaatcgaac tattcctgca gatgctacga ctacggtcat gagttctcgt atgttattgg
     1501 aatggcgaga gacgttttaa tcaatcacac gcgagatatt gatattaatg agccagacgg
     1561 accaacgaat ctttctgcat ggctaagact taagaacaaa gaattacctc cttcactgca
     1621 tcactactaa cagattgcca acgaaatgac ccagcttcgg ctgggttttt tatcaggagt
     1681 tctcatgctc tatagcaata tattggcgca cgcccggcgg tgtgcgcctg cggagtcgtg
     1741 cggcttcgtg ataagcacgc cggaggggga acggtatatc ccttgtgtga atatctctgc
     1801 agagccggag gcgtattttc gtatcgcacc ggaagactgg ctgcgggcag agatgcaggg
     1861 ggagattgtg gcactggtcc acagtcatcc cggtgg
//
LOCUS       DAZKIV010000319         1895 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZKIV010000319 DAZKIV010000000
VERSION     DAZKIV010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.321"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1895
                     /locus_tag="ACHOT6_004908"
     CDS             <1..>1895
                     /locus_tag="ACHOT6_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HFP9949945.1"
                     /translation="NKAAQRSITAPDRIGEGKSWVYQRDTMIERGDNSGVHQYGPAEH
                     FTHIISDKPSPKDEYIAYAINIPDYELAADVYNINVTSPSEQQETFKILINPEHLRQT
                     LERKSLTAVQKSQCEIITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVT
                     TFRADGPETPEEEKGYWFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYT
                     FNFMGIGGSGKNDLDYPSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIH
                     PEDIHLTAKDFEASKDNISKDEWENLHALKEKRLNGMPKTTPKSDQVIMLQNQYVREM
                     RKYGVRGLRYDAAKHSKHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYL
                     VTCQLDEQQMSSLLYERDDLSAIDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSE
                     RRILININHDFPNNGNLFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTE
                     IKNYDGSTRVGGEAWLNKGCSTYQQLYNEFHALYIDKAGIWSAFEGIFATKNVLAFSR
                     GDSVNINHSPHDGLVIINKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPP
                     GKVFTLR"
BASE COUNT          664 a          366 c          359 g          506 t
ORIGIN      
        1 tgaataaagc agcacagcgt agcattacag cgccagacag gataggagaa ggaaaatcat
       61 gggtttatca aagagataca atgattgaaa gaggtgataa cagcggtgtt catcagtatg
      121 gtcctgctga acatttcacc cacattatat ctgacaaacc gtccccaaaa gatgagtata
      181 ttgcatatgc tattaacatt cctgactatg agctggcagc cgatgtatat aatattaacg
      241 tgacgtcacc ttccgaacag caagaaacat ttaaaatact aatcaatcca gaacatctac
      301 ggcaaacact tgagcgtaaa tctcttactg ctgttcagaa atcacaatgt gaaatcataa
      361 cccccaaaaa acctggcgaa gcgattcttc atgcttttaa tgccacctac cagcaaatca
      421 gggaaaatat gtctgagttt gcacgttgcc attatgggta tatacaaatc cctccagtga
      481 caactttccg cgccgacgga ccagaaactc ccgaagaaga aaagggttac tggtttcatg
      541 cttatcaacc cgaagatctt tgtaccatcc ataatccaat gggagatttg caggatttta
      601 ttgcattggt taaagatgct aaaaaatttg gtatcgatat cattcctgat tataccttta
      661 actttatggg aattgggggt agtggtaaaa atgacctgga ttatccctct gctgatatac
      721 gagcgaagat cagtaaagat atagaaggtg gtatccctgg ctattggcaa ggtcaggttt
      781 tgattccatt cattaaagat ccagtaacaa aagaacgtaa acaaatccat ccagaagata
      841 tacatctcac tgcaaaagac ttcgaagcaa gtaaagataa catctctaag gatgaatggg
      901 aaaacctcca tgcattaaaa gaaaagcgtt taaatggaat gcctaaaaca acacccaaaa
      961 gtgaccaggt tattatgttg caaaatcaat acgttcgtga aatgcgaaaa tatggcgtac
     1021 gaggtttacg ttatgatgcg gcaaaacact caaaacatga acaaatagaa agatcaataa
     1081 ccccaccgct taaaaattat aatgagcggt tacacaatac taacttattt aacccaaaat
     1141 atcataaaaa agccgttatg aattacatgg aatatctggt aacttgtcag ttggatgaac
     1201 aacaaatgtc atcactgctt tatgaaagag atgatttaag cgccattgat ttttcattgc
     1261 tcatgaaaac gataaaagcc ttttcatttg gtggagatct ccaaaccctt gcatcaaaac
     1321 cgggttccac aatctcaagt atcccatcag aaagacggat attgattaac attaaccacg
     1381 attttcctaa caatggtaat cttttcaatg actttctatt taaccatcaa caagatgaac
     1441 aattagcaat ggcatatatg gccgctctgc cgttcagcag gcctttagtt tactgggatg
     1501 gccaagtatt aaaatcaacg actgaaatta aaaattatga tgggtccacg cgtgttggcg
     1561 gtgaggcgtg gcttaataaa ggttgctcta cctatcagca gctctacaat gaattccacg
     1621 cattatatat agataaagca ggaatatgga gcgcatttga gggtatattt gcaactaaaa
     1681 acgttctggc ctttagtcgt ggggattctg tgaacattaa tcactctcct catgatggac
     1741 tagttataat aaataaagga aacgaagaag ttgaaggtac ctggcctaac aaattgcaac
     1801 ctggaatata taaaaacatg gggagtaata gcgttaacat tattattaat aatacccgaa
     1861 aaattatccc ccctggtaaa gtatttacgc ttaga
//
LOCUS       DAZKIV010000320         1854 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZKIV010000320 DAZKIV010000000
VERSION     DAZKIV010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.322"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..376
                     /gene="bssS"
                     /locus_tag="ACHOT6_004909"
     CDS             122..376
                     /gene="bssS"
                     /locus_tag="ACHOT6_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFP9949946.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            491..1609
                     /gene="solA"
                     /locus_tag="ACHOT6_004910"
     CDS             491..1609
                     /gene="solA"
                     /locus_tag="ACHOT6_004910"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFP9949947.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1657..1770
                     /locus_tag="ACHOT6_004911"
     CDS             1657..1770
                     /locus_tag="ACHOT6_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFP9949948.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          475 a          447 c          443 g          489 t
ORIGIN      
        1 ctgttccccc ctttaaaaat tcggtgaata cccttactta ttggtatact gaagttgctt
       61 cagataaaaa cgcattgaac ctcgaataac gttgtctagt aacacgaatt agggggccat
      121 gatggaaaaa aataatgaag tcattcagac tcatccgctc gtagggtggg acatcagcac
      181 cgttgatagc tatgatgcgc tgatgttgcg tttgcactac cagaccccaa ataagtccga
      241 gcaggaaggg actgaagttg gtcagacgct ctggttaacc actgatgttg ccagacaatt
      301 tatttcgata ttagaagcag gaatcgccaa aattgaatcc ggtgatttcc cggtaaacga
      361 gtatcggcgt cattaacatg acatgaccat ccacccaaaa ggcacctcac cggtgccttt
      421 accatttctg ggttgtatat ctgcattcag ttaattactc tgcttaaacc atgagagcag
      481 agagaaaaaa atgaaatacg atctcatcat tattggcagc ggttccgtag gcgctgccgc
      541 cgggtattat gcaacccgcg ccggtttaaa cgtgctaatg accgacgccc atatgccacc
      601 gcatcaacac ggcagccacc atggcgatac gcgattaatt cgccatgctt atggtgaagg
      661 cgaaaagtat gtcccgctgg tcctccgcgc gcaaacgctg tgggatgaac tttcccgcca
      721 caacgaagat gatcccattt ttgtacgctc tggtgtcatt aacctcggcc cggctgactc
      781 cgcatttctc gccaacgtcg cccacagcgc cgaacagtgg caactcaacg ttgaaaagct
      841 cgatgcgcaa gggattatgg cccgctggcc agaaatacgc gtcccggaca actacatcgg
      901 cttatttgag actgattccg gttttttgcg cagcgaactg gcgattaaaa cctggatcca
      961 actggcgaag gaagcgggct gtgcgcaact gttcaactgc ccggtcaccg caattcgtca
     1021 tgacgatgat ggcgtaacta ttgaaacggt tgacggtgag tatcaggcga aaaaagcgat
     1081 tgtctgcgcg ggaacatggg taaaagacct gctcccggag ctgcctgtcc agcccgtacg
     1141 taaagtattt gcctggtatc aggccgatgg ccgctatagc gtgaagaata aattcccggc
     1201 gtttaccggt gaactgccca atggcgatca atattatggt tttccggcag aaaacgacgc
     1261 gttgaagatt ggcaaacata acggaggcca ggttatccat tcagcggatg aacgtgttcc
     1321 gtttgcggaa gtggtcagcg atggttcgga agccttcccg ttcttgcgca atgtattgcc
     1381 gggtatcggt tgctgcctgt acggcgctgc ctgcacctat gataattcgc ctgacgaaga
     1441 ttttattatc gataccctac ccggccacga taatacactg ctcattaccg gcctgagtgg
     1501 gcacggtttt aaatttgcgt cagttttagg ggaaatagct gccgattttg cgcaagacaa
     1561 aaaaagcgat tttgatttga cgccattcag gctttcccgc ttccaataat catcattacg
     1621 gcctccagaa tgaggccgtt tttacaggag tatttaatgc gtcggctgtt gcactatctc
     1681 atcaataata ttcgcgagca tctgatgctt tatctttttc tctggggatt gttggcaatt
     1741 atggatctga tctatgtgtt ttatttttag ttttccgcac tgacataaac tgacaaatat
     1801 ttaatctccc tttcatttag cgtgaaaaat tgaatgagat tattcctgct tcta
//
LOCUS       DAZKIV010000321         1829 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZKIV010000321 DAZKIV010000000
VERSION     DAZKIV010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.323"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1355
                     /locus_tag="ACHOT6_004912"
                     /pseudo
     CDS             1..1355
                     /locus_tag="ACHOT6_004912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1368..1829
                     /locus_tag="ACHOT6_004913"
                     /pseudo
     CDS             1368..1829
                     /locus_tag="ACHOT6_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          584 a          361 c          351 g          533 t
ORIGIN      
        1 ataacaacct gatcacataa tcgggtacga ttttatccga taactataat gctataactc
       61 tgggaaatgg ttggaataca ccgctgggtg ccatctcttt tgacgctacc cgatcgagta
      121 gtaagttaaa taatgatata acacacgaag gtacgagcta tcaggtcgcc tataataaat
      181 atctagtgca aacggctact cactttagcg tagccgcctg gcgttatgct tcgcaggatt
      241 acaggacatt cagcgaccat ctttacgaaa atgataaaat aaatcatcag agtgactatg
      301 acgattttta tgatattagc agaaaaaata gcctttctgc caatatcatg caacctttat
      361 ctaaaaatct gggaaatgta tcattaagtg cgctttggcg gaattactgg gggcgaagtg
      421 gaaatgctaa agattaccaa ctcagttact ccaataactg gcaacacatt agttatactt
      481 tctctgccag ccaatcttat gatgaaaata ataaagaaga ggagcgtttt aatctgttta
      541 tctccattcc tttctactgg ggggatgata ttgccaaaac acgtcaccaa attaacttat
      601 cgaattcgac ctcattttcc aaagatggct attcctccaa caatactgga attactggca
      661 tagccggtga acatgatcag ttaaattatg gtatatatgt taatcagcaa caacaaaata
      721 atgatacctc gcttggtacg aatttaagct ggagaactcc catcgccata atagatggca
      781 gctatagtca ttctaaaaac gcctggcaaa gtggtggaag tattagtagt ggattagttg
      841 tctggcccgg tggtattaat atcactaacc agttatccga tacatttgca attctggatg
      901 cgcctgggtt agaaggtgca catattaatg gacaaaaata caatcgaaca aacagcaaag
      961 gccaggttgt ttacgacctg attatacctc atcgtgaaaa ccatctggta cttgatatag
     1021 caaacagtga aagtgaaacc gaattgcagg gcaatcgtca aattattgcg ccttaccgtg
     1081 gagcagtttc ttatgtgcag tttacaactg accaacgtaa gccctggtat atacaggcac
     1141 tgcgtcccga tggttcgcca ttaacctttg gctatgacgt actggatttc caggaaaaca
     1201 atattggagt cgttggccag ggtagtcgcc tttttattcg cgtagatgaa attcccactg
     1261 gcataaaagt tgctctcaat gatgaacaga acttattctg tactattact tttcaacacg
     1321 ttatcgatga aaacaaaaca tatatatgcc agtaaaagga gtttactatg aagtggcttt
     1381 tattaatcac cttgagtcta tattctttta ttgtgcaaag tgctccctgt gcattgacca
     1441 atgtcggtga acaacgtgga acatatatac tcaagccact gagtatgaaa ggtaatctaa
     1501 ccgccgaaaa aattgaaaac accggaaata tcattgagtg tacagacttg caagagacaa
     1561 ccaacgccat taaagttcat tttttaacac agaacgcttt aaggtttaaa gtcggaagtc
     1621 gtcattatat tattaatttc ccctttgaat ctgacggtaa aaaaaataga gcttccaggg
     1681 aagtcatctt ataatttaga agaaatgcta tctgcgataa atttcaccat tccatattct
     1741 atcgcatctg tatcagatag ttctaacacc gcagacattt ttcctggaac tccttttcct
     1801 ttgttgttcg aaattgaagt gagttcatg
//
LOCUS       DAZKIV010000322         1826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZKIV010000322 DAZKIV010000000
VERSION     DAZKIV010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.324"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(94..1602)
                     /gene="putP"
                     /locus_tag="ACHOT6_004914"
     CDS             complement(94..1602)
                     /gene="putP"
                     /locus_tag="ACHOT6_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFP9949949.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
BASE COUNT          497 a          559 c          406 g          364 t
ORIGIN      
        1 cgctccctgc accattcaat ttatatggcg aggcacatta agggagcgca acccgctgct
       61 gaagccaaac aaaaccgccg caggctaagt cccttagctt tcctgcaacc gtgacggcgg
      121 agccgaatga tagtgcgcat cggcctcggc aaagcgtttt tgcatcgccg ctgacggcgc
      181 tttacccagc aaactaaaca ctacaatccc gatactgccg aagatgaagc ccggaataat
      241 ttcgtagagt cccagccagc cgaactgctt ccagacgata accgtcagcg caccgatgat
      301 catccccgcc agcgcaccgt tgcgcgtcat gcgtgaccac atcaccgaga acagcaccac
      361 cggaccaaac gccgcgccaa agcctgccca cgcgtagctc actaagccca gcacgcggtt
      421 ttccgggttt gccgccagcg caatcgccac cagcgccacc accagcacca tcacacgtcc
      481 tacccacacc agctctttct ggctggcatg tttacgcaga aacgctttgt acaaatcttc
      541 ggtaatcgca ctggagcaca ccagcagctg gcagcttaac gttgacatta ccgccgccag
      601 aattgccgac agcaaaatcc cggcaatcca cgggttaaac agaatttgcg ccagttcgat
      661 aaacacacgt tcagcattct ggtttaccgc acctgctacc gccgggtgct cattaaagta
      721 agcaatacca aagaagccga cagccaccgc ccctgccagg cagaggatca tccaggtcat
      781 actgatacga cgcgcatgga caatgctgtg gtgagaatcc gccgccataa aacgcgccag
      841 gatgtgcggc tgcccgaagt aacccagccc ccagcccatc agcgagataa tggcaacaaa
      901 gttcagacct ttgagcatat caacgttttc gatgctcttt tgtttgatca cttccagcga
      961 gtcaccaaag ccaccgacac tgataatgac gataaccggc gtcaggatca gggcaaaaat
     1021 catcaggctg gcctgtacag tatcagtcca gctcaccgcg aggaaaccgc caataaaggt
     1081 gtaaaggatc gtcgccgcag ctcccgccca cagagccgtt tcgtagctca tgccaaaggt
     1141 actttcaaac agacgcgcgc ctgccacaat gcccgaagcg caataaatgg tgaagaacag
     1201 caaaataacc agcgcggaga taatgcgcaa aatgcggctt ttatcttcaa agcgcccggt
     1261 gaaataatcc ggcagggtta aggcgttatt gttgtattcg gtatgcacac gcaaccgccc
     1321 ggccaccagc ttccagttga tccacgcgcc taatgtcagg ccaatggcga tccagctttc
     1381 ggaaatcccg gaaagaaaaa ccgcgcccgg caaccccatt aacagccagc cgctcatatc
     1441 cgacgccccc gccgataatg ccgtcacgaa tggcccaaga ctacgaccgc ccagaatata
     1501 gtcgtcaaag tttttcgttg atcgccaggc gataaaccca atcaatatca tgccaaagat
     1561 atagacacaa aatgtcacca acatcggtgt gctaatagcc atctaaagtc tccaaaaaat
     1621 tattatcggc aatgtcgaaa cttgccgtta tatctgccac cggaacgggg taacagagtt
     1681 tatgttttac cagggcgacc gtatcctgcc ggaagcgctg gttattcaca atcgatttaa
     1741 cacaccattt acattaaatt ttagtgctca gcgacactat ttttcatcag gttgcactct
     1801 ctcacatttt ttgcggttgc accttt
//
LOCUS       DAZKIV010000323         1790 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZKIV010000323 DAZKIV010000000
VERSION     DAZKIV010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.325"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            432..1046
                     /gene="evgA"
                     /locus_tag="ACHOT6_004915"
     CDS             432..1046
                     /gene="evgA"
                     /locus_tag="ACHOT6_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFP9949950.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1051..>1790
                     /locus_tag="ACHOT6_004916"
     CDS             1051..>1790
                     /locus_tag="ACHOT6_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HFP9949951.1"
                     /translation="MKFLPYFFLLWCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTR"
BASE COUNT          568 a          355 c          336 g          531 t
ORIGIN      
        1 aattaatctg ttccactatt atctctcatt tctcatagat gaaatttatg aattgaatga
       61 aaagagcatt cattcttcat atcaaacaca gtgcatgtgt tgcacattat cccaggaacg
      121 ttggaggcct aatttaaatg aggtgcaaaa aaaacaacag aggactaaac cgtggctttt
      181 gcaatacaat tcttacgcct gtaggattag taagaagact tatagtgcta acttgaaact
      241 ataaatcatc ggtacaatcc ctgattttat tgttgacatt tcatttatgc cgactattta
      301 tatggtatac ttgtcgaatt atcttaaagg aagctcagat tttcttattt ttattgagat
      361 aatgagatga cgccttatgt ctgtattact acagggagaa gggagatgct tcattgcaaa
      421 gggaataatc tatgaacgca ataattattg atgaccatcc tcttgctatc gcagcaattc
      481 gtaatttatt gatcaaaaac gatattgaaa tcttagcgga gttgactgaa gggggaagcg
      541 ccgttcagcg ggtggaaaca cttaagcctg atatcgtcat cattgatgtc gatatccccg
      601 gagttaacgg tatccaggtg ttagaaacgc tgaggaagcg ccaatatagc ggaattatta
      661 ttattgtctc cgctaaaaat gaccattttt acgggaaaca ttgtgctgat gctggcgcta
      721 atggattcgt gagtaaaaaa gaaggcatga acaatatcat tgcggctatt gaagctgcaa
      781 aaaatggcta ctgctatttc cccttctctc tcaaccggtt tgttggaagt ttaacgtccg
      841 accagcaaaa actcgactcc ttatcgaaac aagaaattag tgtcatgcgg tatattcttg
      901 atggcaagga taataatgac attgctgaaa aaatgttcat cagcaacaaa actgtcagca
      961 cttataaaag tcgcctgatg gaaaaattag aatgtaaatc actgatggat ctttacacat
     1021 tcgcgcaacg taacaaaatc ggctaatcac atgaagtttt taccctattt ttttcttctc
     1081 tggtgtggtc tttggtcgac cataagtttc gcagacgaag attacatcga atatcgtggc
     1141 atcagtagta acaaccgtgt cacacttgat ccagtacgtc tgagcaacaa ggaattacgt
     1201 tggttagcga gcaaaaaaaa tcttgtgatt gcagtacaca agtcccaaac ggctacgttg
     1261 ttgcataccg attcgcagca acaggttcgt ggtattaatg ctgattattt aaatctttta
     1321 aaaagagcgt taaatatcaa attaacactc cgggaatacg cagatcatca aaaagcaatg
     1381 gacgcgcttg cagaaggtga agtcgatata gtgttatcac atttagttac ttcgccgcct
     1441 cttaataatg acattgctgc aaccaaacca ttgataatta cctttccggc gctggtaacc
     1501 acccttcacg actcaatgcg accgcttacc tcaccaaaac cagtaaatat tgctcgggta
     1561 gcaaattacc ccccagacga ggtaattcat caatcatttc caaaagcaac aattatctct
     1621 tttacaaatt tatatcaggc attagcatcc gtctcagctg ggcacaatga ttactttatt
     1681 ggtagtaaca tcattaccag cagtatgatt tcccgctatt tcactcactc cttaaatgta
     1741 gtgaaatatt ataactcgcc gcgtcaatat aattttttct tgaccagaaa
//
LOCUS       DAZKIV010000324         1767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZKIV010000324 DAZKIV010000000
VERSION     DAZKIV010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.326"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..512
                     /locus_tag="ACHOT6_004917"
     CDS             <1..512
                     /locus_tag="ACHOT6_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFP9949952.1"
                     /translation="GGWSLYAYPLNPVQHVDPLGLSTMIIGNGPVPDNPFGHAAAANR
                     YGLMSSGTGDEMGASVSDYFKKMQPRRDTWIWIIDTTDEEEQCMMNKAIDLNKKLKTI
                     NLGPLPVANNCFSRTNMIFEACGFSNPSMNTNAPISLQVLGELYGYQRYFMPKGPFTG
                     FPFEFQEVK"
     gene            513..911
                     /locus_tag="ACHOT6_004918"
     CDS             513..911
                     /locus_tag="ACHOT6_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000425544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949953.1"
                     /translation="MEMLGKILVIIISMIIISCDYYNAGMDYQDNIALEQVVTWDSDN
                     IFTPNIFEHNGRCFMKKNETWMLLNGDATPNIRTMSNKKIECLEKSEVEWASRVCDSI
                     TDKERENLKALICSKPAKSVNTGKRPALGY"
     gene            954..1529
                     /locus_tag="ACHOT6_004919"
                     /pseudo
     CDS             954..1529
                     /locus_tag="ACHOT6_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014622.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1556..>1767
                     /locus_tag="ACHOT6_004920"
     CDS             1556..>1767
                     /locus_tag="ACHOT6_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949954.1"
                     /translation="MSDFDIVSSIDWHDGVLLEVKTTFKESHFNMTLFIDIYKSENIR
                     EKMIVEFSDIDDLVFAMDSAELIDNH"
BASE COUNT          575 a          293 c          414 g          485 t
ORIGIN      
        1 cggggggatg gagtctgtat gcgtatccgc tgaatccagt gcaacacgtt gatccattag
       61 ggcttagtac tatgataata ggtaacggcc ccgttcctga taatcctttt gggcatgctg
      121 cggcggcaaa tcgatatgga ttaatgagtt cgggaactgg ggatgagatg ggagcctctg
      181 tctctgatta ttttaaaaag atgcaacccc gcagagatac ctggatttgg ataatagata
      241 caacagatga agaagaacaa tgtatgatga ataaggcaat agaccttaat aaaaaactaa
      301 aaactattaa cctcgggcct cttcctgttg caaataattg ttttagccgg acgaacatga
      361 tttttgaggc gtgcgggttt agcaacccct ctatgaatac aaatgcgcct ataagcctac
      421 aagtgctcgg tgagttatat ggatatcaaa gatattttat gcctaaaggt cctttcactg
      481 gattcccatt tgaatttcaa gaggtaaagt aaatggaaat gcttgggaag atattagtga
      541 taattatttc catgataatt atatcttgcg attattataa tgctggcatg gactatcaag
      601 ataatatagc cctcgagcaa gtcgtaacat gggattctga taatatattc acacctaata
      661 tctttgaaca taatggaaga tgttttatga agaaaaatga aacatggatg ttattaaatg
      721 gagatgcaac accaaacatt agaacaatga gcaataagaa aattgaatgc ctggagaaga
      781 gtgaagtaga atgggctagt agggtttgcg acagtatcac agacaaggaa agggaaaatc
      841 tgaaggcatt aatatgtagc aaaccagcta aatcagttaa tacaggcaaa agaccagcgt
      901 taggatattg aaaccggcac tgcactatat cactattaca atattctgag tgactagagc
      961 aactgctccg ccatccgggc tagcagtatg acgacgaatc cgaactgcac tacaactggc
     1021 agcggtacta caatccggga cagggacgat atatcaccca ggacccgata gggctggcgg
     1081 ggggattaaa cccgtacagt tatgttatga acaatcctgt gcaatggatt gatccattgg
     1141 gattagctca tacttcagga aaatggaagg actgtggtaa aggttgtcga atccgaatag
     1201 atggcgatgc tgttggtgac ggaagacatt tgcattggga gtgtaaaaat gggagtggtg
     1261 taatggggga atttggaggt atgtcccatg gtgaggatta ctcatcagca ccaaatagca
     1321 ttaagaaatg tgcaaaaaaa tatggtttcg agccagaacc aagtaaagat ctgtcatgca
     1381 aaacctccgg gctatcaact actgatgcgg ctcttgcaac tggtgctaca attggcacag
     1441 ggtatgttat atatagagtg attcgattta taccttcact tgcaccacct ctttggtggt
     1501 caatacctgc aaacgttgtt actccatgat taaaacggat aatggagatt atataatgag
     1561 cgattttgac attgtgagtt caattgactg gcatgatggg gttttgttag aagtaaaaac
     1621 gacattcaaa gaatctcatt ttaacatgac gttatttatt gacatttata aaagtgagaa
     1681 tattagggaa aaaatgatag ttgaattttc tgatatagat gatttagtgt tcgctatgga
     1741 ttctgctgag ttaatcgata atcacaa
//
LOCUS       DAZKIV010000325         1765 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZKIV010000325 DAZKIV010000000
VERSION     DAZKIV010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.327"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1249
                     /locus_tag="ACHOT6_004921"
     CDS             <1..1249
                     /locus_tag="ACHOT6_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9949955.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYG
                     WDGDRLTTIQNDRTRIQTIYQPGSFTPLIRVETATGEQAKTQRRSLADTLQQSGGEDG
                     GSVVFPPVLVQMLDRLESEILADRVSEESRRWLASCGLTVEQMQNQMDPVYTPARKIH
                     LYHCDHRGLPLALISKEGTTEWCAEYDEWGNLLNEENPHQLQQLIRLPGQQYDEESGL
                     YYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYG
                     ARPNKPPTPDPSKLPDIAKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVK
                     PNLTCTPQDDSQYPGMDTKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPAT
                     AGIDDLGEILAKMKQRTSRGIRK"
     gene            1246..1765
                     /gene="ybfC"
                     /locus_tag="ACHOT6_004922"
                     /pseudo
     CDS             1246..1765
                     /gene="ybfC"
                     /locus_tag="ACHOT6_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
BASE COUNT          516 a          401 c          463 g          385 t
ORIGIN      
        1 agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg
       61 ggtatggcgg cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccgcaagt
      121 gacctggtac ggctgggacg gcgaccgcct gaccacgata cagaacgaca gaacccgcat
      181 ccagacgatt tatcagccgg ggagcttcac gccactcatc agggttgaaa ccgccaccgg
      241 tgagcaggcg aaaacgcagc gccgcagcct ggcggatacc cttcagcagt ccggcggcga
      301 agacggtggc agtgtggtgt tcccgccggt gctggtgcag atgctcgacc ggctggaaag
      361 tgaaatcctg gctgaccggg tgagtgagga aagccgccgc tggctggcat cgtgcggcct
      421 gacggtggag cagatgcaaa accagatgga cccggtgtac acgccggcgc gaaaaatcca
      481 cctgtaccac tgcgaccatc gcggcctgcc gctggccctt atcagcaagg aagggacaac
      541 agaatggtgc gcagaatacg atgaatgggg caacctgctg aatgaagaga acccgcatca
      601 gctgcagcag cttatccgcc tgccggggca gcagtatgat gaggagtccg gcctgtatta
      661 caaccgccac cgctattatg acccgctgca ggggaggtat atcactcagg atccgattgg
      721 gctgaagggg ggatggaatt tttatcagta tccgctgaat ccggttcagt atatagattc
      781 aatgggactg gcatcaaaat atggacactt aaataatggc ggatatggag cgagacccaa
      841 caaaccgcct acgcccgatc caagtaaatt gccggacata gcgaaacaat taagactgcc
      901 atatcctatt gaccaggcca gtagtgcgcc taatgttttc aaaacattct tcagagcatt
      961 aagcccttac gactacacac tgtattgcag gaagtgggta aaaccaaatc tgacttgtac
     1021 gccacaggat gattcccagt atccagggat ggatacaaag acagcaagtg attacctgcc
     1081 acagacaaat tggccaacaa ctcaattacc accaggatat acttgtgcag aaccctattt
     1141 attcccagac attaataaac ccgatgggcc agcaacagca gggatagatg atttgggtga
     1201 aattttagct aaaatgaaac agagaacatc gagaggaata agaaaatgaa aagagttttg
     1261 ttctttttgc tgatgatatt tgttagtttt ggtgttatag ctgattgcga aatacaagct
     1321 aaagatcatg attgttttac tattttcgct aagggtacta ttttttccgc gtttcctgtc
     1381 ttaaataata aagccatgtg gagatggtat cagaatgagg acatcggtga gtattattgg
     1441 caaacagaac tgggtacatg taaaaataat aaattcacac caagcggggc gagactctta
     1501 attcgcgtcg ggtcactacg ccttaatgaa aatcatgcca tcaaaggaac gttgcaggag
     1561 ttgataaata cagcagaaaa aacggcgttt ctcggcgatc ggtttagaag ttatataaga
     1621 gcgggtatct atcaaaaaaa gagcagtgat ccagttcagt tgttggcagt ccttgataat
     1681 tcaatcatgg taaaatattt taaagatgag aaaccaactt atgctcgaat gacagcacac
     1741 tcccgaacaa aaatgagtct tacga
//
LOCUS       DAZKIV010000326         1759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZKIV010000326 DAZKIV010000000
VERSION     DAZKIV010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.328"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..486
                     /locus_tag="ACHOT6_004923"
     CDS             <1..486
                     /locus_tag="ACHOT6_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFP9949956.1"
                     /translation="HGLFRVIPIKGASVYGKPVASMPRKRNKNGVYLTEIGTDTAKEQ
                     IYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQVEKWVDGRKKILWDSKK
                     RRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAATNKKTLADYARALSGED
                     E"
     gene            483..689
                     /locus_tag="ACHOT6_004924"
     CDS             483..689
                     /locus_tag="ACHOT6_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9949957.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            686..>1759
                     /locus_tag="ACHOT6_004925"
     CDS             686..>1759
                     /locus_tag="ACHOT6_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9949958.1"
                     /translation="MKTSTIPTLLGPDSMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     PSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSL"
BASE COUNT          418 a          457 c          545 g          339 t
ORIGIN      
        1 catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggaaa gccagtggcc
       61 agcatgccac gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg tacggatacc
      121 gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga accgcttccc
      181 ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc gcagcagctg
      241 actgctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact gtgggacagc
      301 aaaaagcgac gcaatgaggc gctcgactgc ttcgtttatg cgctggcggc gctgcgcatc
      361 agtatttccc gctggcagct ggatctcagt gcactgctgg cgagcctgca ggaagaggat
      421 ggtgcagcaa ccaacaagaa aacactggca gattacgccc gtgccttatc cggagaggat
      481 gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac ctgatgacag
      541 gaaaacgggt ggcaacggta cagaaagacg gacggcgagt ggagtttacg gccacttccg
      601 tgtctgacct gaaaaaatac attgcggagc tggaagtgca gaccggcatg acacagcgac
      661 gcaggggacc tgcaggattt tatgtatgaa aacgtccacc attcccaccc ttctggggcc
      721 ggacagcatg acatcgctgc gtgaatatgc cggttatcac ggcggtggca gcggatttgg
      781 tgggcagttg cgggcgtgga acccaccgag tgaaagtgtg gatgcagccc tgctgcccaa
      841 ctttacccgt ggcaatgccc gcgcagacga tctggtacgc aataacggct atgccgccaa
      901 cgccatccag ttgcatcagg atcatatcgt cgggtctttt ttccggctca gtcatcgccc
      961 aagctggcgc tatctgggca tcggggagga agaagcccgt gccttttccc gcgaggttga
     1021 agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg agcgaaaacg
     1081 cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg gtgaactgtt
     1141 cgttcaggcc acctgggata ccagtccgtc gcggcttttc cggacacagt tccggatggt
     1201 cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact gccgtgccgg
     1261 tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg acgggtatcc
     1321 tggctggatg ccgcagaaat ggacatggat accccgtgaa ttacccggcg ggcgcgcctc
     1381 gttcattcac gtttttgaac ccgtggagga cgggcagacc cgcggtgcaa atgtgtttta
     1441 cagcgtaatg gagcagatga agatgctcga cacgctgcag aacacgcagc tgcagagcgc
     1501 cattgtgaag gcgatgtatg ccgccaccat tgaaagtgag ctggatacgc agtcagcgat
     1561 ggattttatt ctgggcgcga acagtcagga gcagcgggaa aggctgacgg gctggattgg
     1621 tgaaattgcc gcgtattacg ccgcagcacc ggtccgtctg ggaggcgcaa aagtgccgca
     1681 cctgatgccg ggggactcac tgaacctgca gacggctcag gacacggata acggctactc
     1741 cgtgtttgag cagtcactg
//
LOCUS       DAZKIV010000327         1728 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZKIV010000327 DAZKIV010000000
VERSION     DAZKIV010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.329"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..672)
                     /gene="sctT"
                     /locus_tag="ACHOT6_004926"
     CDS             complement(<1..672)
                     /gene="sctT"
                     /locus_tag="ACHOT6_004926"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFP9949959.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASWNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLARYASQLNAFAISLTV
                     KSALAF"
     gene            complement(674..934)
                     /locus_tag="ACHOT6_004927"
     CDS             complement(674..934)
                     /locus_tag="ACHOT6_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9949960.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIAVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(944..1606)
                     /locus_tag="ACHOT6_004928"
     CDS             complement(944..1606)
                     /locus_tag="ACHOT6_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFP9949961.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLSI
                     NP"
     gene            complement(1596..>1728)
                     /locus_tag="ACHOT6_004929"
     CDS             complement(1596..>1728)
                     /locus_tag="ACHOT6_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFP9949962.1"
                     /translation="PESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNNNVK"
BASE COUNT          602 a          376 c          278 g          472 t
ORIGIN      
        1 aaatgccagc gcacttttga cagtcagtga aatggcaaaa gcatttagtt gtgaagcata
       61 acgtgccagc aatcccagga cagcctcacc gcccagcata acggcaatca cggggcttgc
      121 gtaaacgata gtatgagtca ttatgttatt aatatatgag aataaaggct ctaatttagg
      181 aaaattaaaa ttgcctgaag gccataaatt ataactttgc caaagagttt ccagaataaa
      241 gttcattcca cctttagtaa gataaaccgc agcagagaaa aggttaaata atcttgctaa
      301 ttccgaggta tcgacacctg tagccggatc caaagtacta cttaatgttg cacctcgttg
      361 gttatcgata aagctaccta tagcaaggaa aatccaaaac ggggatgcta ataagcatgc
      421 aataaacagc cctatgataa tttctctggc agcgaacaaa gcaatatcga tttcatttaa
      481 gttcaataat tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg
      541 gtgacgaact actgttggta tattccaact ggcgaaaaat ggtaacaaat agaaagttgg
      601 tgccaatcgg caaaaaccga gcgctgtagc agccagcaga ctatgcaact gatagagtat
      661 cgcctccccc atgctatcct ctcgcgaatg ctaaattcag catttcgata ccaaatgaat
      721 ataatttttc accataccac ccgctcatta aaaaaaagca aatacttacg cacaataatt
      781 tcacgccaaa tggcaacgtc tgttcttgca actgcgttac cgtttgaaaa agccctacca
      841 ataaaccaac aaatgtcgca actgcaatag gtcctgcaga cataactaaa atcagataca
      901 gcgccctatt ccccgcaaaa acaatatcgt ccatattttt tcctcaggga ttaatggata
      961 ggtcaaaata ctgtaaaatg agcccttttg ataacattgt ccaaccgtcc atcgcgacaa
     1021 acaaaattaa ttttatcggt gtagatatgg ttaccggact catcatcata cctaacgtta
     1081 acagaacgct ggatataacc agatccacta cgacaaaggg taaatagata taaaaaccta
     1141 taataaatgc actttttatc tcactcaatg catatgcggg cagtaacgag aaaattgaaa
     1201 tattatcatc atcgattatc tcttcattgt cttcagaact attcaccttc tgaatctttt
     1261 caaaaaagct aaccaactca ggttcagaat attttatcaa atatgattta taaccactca
     1321 tgcctgtctc aacaaaatta acaaccgatg ctacattatt gaatgaaaga ttctcattct
     1381 gactgttatt atatatttcc ttcccaaccg gcatcattac gaacattgac aatagcaaag
     1441 caaccccatt aagtgtcata tttgatggca cttgctgaag tcccagcgcg tttcggacaa
     1501 taacaaaaac aatcgagaat ttaataaaac atgtcccgga tgctattata aacggcaata
     1561 gtgtaaataa tgataatatc gctatcagcg aaatgctatt tgacattatt attccctgat
     1621 aaccaagaat gaatttctac gcccaattta tcatccactt caacaagttc gccatagcca
     1681 gttagcgcac catttacacg tatctctata tttttctcag attcagga
//
LOCUS       DAZKIV010000328         1718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZKIV010000328 DAZKIV010000000
VERSION     DAZKIV010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.330"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..70
                     /locus_tag="ACHOT6_004930"
     CDS             <1..70
                     /locus_tag="ACHOT6_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949963.1"
                     /translation="VMLVAIFFSTAWRVLTLSGLVG"
     gene            298..483
                     /locus_tag="ACHOT6_004931"
     CDS             298..483
                     /locus_tag="ACHOT6_004931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFP9949964.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPA
                     PDWQTPLNGIISSSENG"
     gene            complement(908..1135)
                     /locus_tag="ACHOT6_004932"
     CDS             complement(908..1135)
                     /locus_tag="ACHOT6_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFP9949965.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDQKGSENFSAWVIES
                     CRRELAADIKYARQLTIKKNDTQYALRWLFI"
     gene            1177..1542
                     /locus_tag="ACHOT6_004933"
     CDS             1177..1542
                     /locus_tag="ACHOT6_004933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFP9949966.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSVSETASGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          399 a          402 c          479 g          438 t
ORIGIN      
        1 agtcatgctg gttgccattt tcttttccac cgcctggcga gtcctgacgt tatcgggact
       61 ggtggggtga aagagagatg aaccgtgttc tgtgtgtggt gattattgtc ctggcggttg
      121 gctgtggtgc gctgtggctg gcaacaaacc attaccgtga caacgcgctc acctacaaag
      181 cgcagcgcga taaaaaagcc agagagctgg aacaggcgaa tgccaccatt actgacatgc
      241 aggtgcgcca gcgtgatgtt gctgcgctcg atgcaaaata ctcgagggag ttagccgatg
      301 cgagagctga aaatgaaact ctgcgtgctg atgttgccgc tggtcgtaag cgcctgcgga
      361 tcaacgccac ctgctccggt accgtgcgtg aagccaccgg cacctccggc gtggataatg
      421 caaccggccc ccgactggca gacaccgctg aacgggatta tttcatcctc agagaacggt
      481 tgatgaccat gcagaagcag ctggaagggg cacaggacta tatccgcact cagtgcctga
      541 actaagtttt gctgatgcgc cgtatcgtcg ccgtattcct gcattaacag agaccgcagc
      601 ccgacaggga gactcctctg cgagagtgtg cggggataat caaaaacgat acacaccggg
      661 gtttaccgcg taaacggagc gcggcgttct cccctcatgg tcgcccgtcc ggtgcgatgg
      721 tggaagaaac tggaatctgt tcaataaaaa aactgccgtg ttggagtcac agcagtaatg
      781 tactgattgg gtagaagatt attattgtta tgctttattt ttattctata tggctgatta
      841 tttcaattcg gaattaatac agctaatgtc tgtgagtttt tataaattca gcaatataaa
      901 gaaatagtta tatgaacagc catcgcagag catactgtgt atcattcttt tttatagtca
      961 actgacgggc atattttatg tctgctgcca gctcccggcg gcaagattca atgacccacg
     1021 cagaaaaatt ttctgaacct ttctggtcaa gagcgatgtt aatttgttca atcatctggt
     1081 ttggaaatcg gatgttgcgg gttgttgttc tgcgggtccg gtttttcgat gacattttct
     1141 ttcctctggt gacaagttat atggcgagga ttttacatgg ctgtgcttcg tacgttaccg
     1201 ggcagaatca aaactctgaa cacccggcgg gtgaatgtcc tgaagggtga acagcgtcgg
     1261 gtcagtggta gtgcccgggt ttccctcaag cgccgtatct ggctcaggga tgccgggcag
     1321 tgctgtctct gtggtcgtgt ggttgacctc tgtgacagtg aactcgatca ccgaattgca
     1381 cttcagttcg gtggtggtaa tgaggagacg aatctctgga cgctctgtac cgaatgccat
     1441 cgccagaagt cagtcagtga aacggcgagt ggtatgccgg acccgacgct gccggaggtg
     1501 tccggaggtc atggcagggc agacgatatc atcggactgt gacccgcccc ggggggggga
     1561 tcatccggcg aaaaaaacga tcgccccgga caccgccccc cctctcacgc agagaaaaaa
     1621 ttcccgtttc agggcagtta acatgttaac tggctgtccg ggcatttttg cggtttttat
     1681 ctttattatt cagtttgttg cgtggaaaaa atgttaac
//
LOCUS       DAZKIV010000329         1671 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZKIV010000329 DAZKIV010000000
VERSION     DAZKIV010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.331"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..162
                     /locus_tag="ACHOT6_004934"
     CDS             <1..162
                     /locus_tag="ACHOT6_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9949967.1"
                     /translation="GTGYWLLQLLDKVSPSQWVAIGVLGSLLFGLLTYLTNLYFKIKE
                     DRRKAARGD"
     gene            166..682
                     /locus_tag="ACHOT6_004935"
                     /pseudo
     CDS             166..682
                     /locus_tag="ACHOT6_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001385817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            725..1258
                     /locus_tag="ACHOT6_004936"
     CDS             725..1258
                     /locus_tag="ACHOT6_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9949968.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAIMVDGKPVIPGMKLSKEKCDRVNAIERDKALAWVEKNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESVLACWGIDR"
     gene            1529..>1671
                     /locus_tag="ACHOT6_004937"
     CDS             1529..>1671
                     /locus_tag="ACHOT6_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
                     /protein_id="HFP9949969.1"
                     /translation="MNMMTVPFHGNSLYVVNHNGEPYVPMKPVVAGMGLAWQSQLAKL
                     RQR"
BASE COUNT          443 a          358 c          457 g          413 t
ORIGIN      
        1 gggacgggat actggttact gcagctgctg gacaaagtct ctccgtccca gtgggtggcg
       61 ataggtgtgc tggggagtct gctgtttggc ctgctgacgt atctgactaa cctgtatttc
      121 aaaattaaag aggaccgtcg caaggctgcc cggggagatt aggtgatgaa ccatgaagaa
      181 atgaatcagc gcttcagtcg tctggaaaat gaaattgctg aactgaataa aaaactgtcg
      241 acgctgatgc cttctgaaga tgaaaaaaaa cgccgcgatg agcagaaatc tctcagagtg
      301 tttcagtatt cataatgata atttcagtga gctggaatgg gagtgtaacc ggccatcctt
      361 tgttgtatcc ggtgatgctg ggaaaataac catctcagaa aatggaaaag taacaccgcc
      421 atcgcaccag catagtgagg tgctcattga atttgccatt gattacctga agaacaataa
      481 aaagcagggg ctgatgaagt gcattggtcg ttgcatggga tatctgcaga tagctgctga
      541 gattgaagcg ctggccagtg gtgcggacaa ggatgcagtt gtgcgggagg ctcttcttcg
      601 tgagtttgac aacccgccct ttaaaaaagt gccggcttac tggtttcatc caggactgac
      661 ttatcttaaa ggacgtatat aagctggctc gttatctgtt gccgataaat cctgataaat
      721 atccatgaac accaaaatca aatacggcct gtcggctgcc gttctggcgc tgattgccgc
      781 tggtgcgcct gcgcctgaca ttctcgacca gtttctggat gaaaaggaag gtaaccacac
      841 cacggcatac cgtgatggtg cgggtatctg gaccatctgc cgaggtgcca tcatggtgga
      901 tggtaagcct gtgattcctg gcatgaagct gtcgaaggaa aaatgcgacc gggttaacgc
      961 cattgagcgt gataaggcgc tggcatgggt ggagaaaaac atcaaagtgc cactgaccga
     1021 accccagaaa gcggggatcg cgtcattctg tccgtacaac attggtcccg gtaagtgttt
     1081 cccgtctacg ttttacagac gaattaatgc aggtgatcga aaaggtgcct gcgaggcgat
     1141 tcgctggtgg attaaggacg gtggcagaga ctgccgtatt cgttcaaaca actgttacgg
     1201 tcaggtatcc cgtcgtgacc aggagagcgt gctggcgtgc tggggaatcg acagataagc
     1261 agaatatttt gctgaaaaat gaggtttgct tacatggacg tataacacga aatcctgcaa
     1321 attggcaaaa tgtaagtgaa taaagtcaaa acagttgttt aacactcagg caccgtaatg
     1381 gtgcctttgt catttctgcg catctcacgc gcatctcaca acacagaacc tttcaggatg
     1441 acccttgagg ataccggttt ggctgtcggt gcctttctgt gggctggatt cctgtgagac
     1501 aaggttcatc actaaaagga aataaccgat gaatatgatg accgtgccgt ttcacggcaa
     1561 ctctctttat gtagttaacc ataatggcga gccatacgtt cccatgaaac ctgtcgttgc
     1621 ggggatgggg ctggcctggc aatcacagtt ggctaagtta agacagcgtt t
//
LOCUS       DAZKIV010000330         1665 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZKIV010000330 DAZKIV010000000
VERSION     DAZKIV010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.332"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(147..656)
                     /locus_tag="ACHOT6_004938"
     CDS             complement(147..656)
                     /locus_tag="ACHOT6_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein EspH"
                     /protein_id="HFP9949970.1"
                     /translation="MSSSLSGITFTTFLTSNHASWNKLTRTLHISNDDILDELQLKAR
                     MQQRHHRVFAGIDDYSIINFRGKDYAVRFIKDNDNYVYKVNKITQESGCFSTIFSFLY
                     GDVTKALESKLNERHITPLSSTWFPRTALEGFLTERGLSSLLRRVQSTEGVNPPENTT
                     LNSLSGVTV"
     gene            complement(687..1604)
                     /gene="escQ"
                     /locus_tag="ACHOT6_004939"
     CDS             complement(687..1604)
                     /gene="escQ"
                     /locus_tag="ACHOT6_004939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE ring protein EscQ"
                     /protein_id="HFP9949971.1"
                     /translation="MKPLSSQLNMKINDFYLPLLPVIGTGRLYITSEGHACHAYFREV
                     SGHGIRFTLTYSGYEGRFWISEEQFIQWCQELFPYSESRLIPEDMIKLMILWVMQTAL
                     PEGDVSVDDVQFTMLNKDVYPVIENNNGENRLNVIILETTVQSLQHLINDNWQFVPHS
                     NALFFDGYIVPGWTDYPVTELRVGDSLRLYHVDDSQERRCWIVINNPLATVKLSDNNL
                     SVTDVLAADPLCTISSETVMNRIYCVSGTVHIDIHMLRNVQKDDIIDSTGYHLFGGCQ
                     LVRNNTTIAYGSIIKINEDFYFTISVVCD"
     gene            complement(1567..>1665)
                     /locus_tag="ACHOT6_004940"
     CDS             complement(1567..>1665)
                     /locus_tag="ACHOT6_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001303723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1106 domain-containing protein"
                     /protein_id="HFP9949972.1"
                     /translation="HVFLYHKKDYVFDKLKEIFPDEAIEFTIEYEN"
BASE COUNT          542 a          354 c          271 g          498 t
ORIGIN      
        1 tcagttcata tttcacaaaa actacagtgc tctatccttg aaatactaat catttcgcct
       61 gcatattctt ttcaaaaaat aatgaaatat cgcccccaga acagaacaca ttctggggag
      121 aaaaagaaaa cagcataaaa taatacttaa actgtcacac ctgataaaga gtttaatgta
      181 gtattctctg gagggttcac accttctgta gactgtacgc gacgtaacaa agaagagagc
      241 cctctttcag ttaaaaagcc ttccagtgca gtgcgaggaa accaggttga agaaagtggc
      301 gtaatgtgtc tttcatttaa tttactttcc agggccttcg taacgtctcc atacaaaaag
      361 ctaaatatcg ttgagaaaca tccactttcc tgagttatct tatttacttt gtatacataa
      421 ttatcattat ctttaatgaa gcggacagca taatcttttc cacgaaaatt gataattgaa
      481 tagtcatcaa tgcctgcaaa aacccggtgg tgacgctgct gcatgcgtgc tttaagttgc
      541 agttcatcta gtatatcgtc attcgatata tgaagagttc gggtcaactt attccatgac
      601 gcatgattgc ttgtcaaaaa agtagtgaat gttatacctg ataatgatga cgacatcata
      661 cacctcccta tataacgcat tcctgattaa tcacatacta cgctaatagt aaaataaaaa
      721 tcttcgttga tttttataat actcccgtat gcgattgtgg tattattccg tactaactgg
      781 cacccaccaa aaagatgata tcccgttgaa tcgataatgt catctttttg gacattacgc
      841 aacatatgta tatcaatatg cactgtgcca ctcacacaat aaatgcgatt catcacagtc
      901 tcgctgctga tagtacataa cggatctgca gccagaacat ccgtgacaga taaattatta
      961 tcgctcagct ttacggttgc caaagggtta ttaataacaa tccagcacct tctctcttga
     1021 ctatcatcaa catgatagag tctcagacta tcaccaactc tcaactctgt aactggataa
     1081 tcagtccacc caggtacgat atagccatca aaaaaaagag cattagaatg tggaacaaat
     1141 tgccaattat cattaatcag atgttgtaaa ctttgtaccg ttgtttcgag tataattaca
     1201 ttaagtctgt tttctccatt attattttct attaccggat aaacatcttt atttagcatc
     1261 gtaaattgaa catcatcaac tgacacatca ccttccggta aggcagtctg cattacccat
     1321 aagatcatga gttttatcat atcttccggt atcagccggc tttcactata cgggaataac
     1381 tcttggcacc actgaatgaa ctgttcttct gatatccaga aacgcccctc atagcccgag
     1441 tatgtcaacg tgaaacgaat accatgaccg ctgacctcac gaaaatatgc atggcaagca
     1501 tgaccttcgg acgtaatata gaggcgaccg gttccgataa ctggcaacaa cggcaaataa
     1561 aaatcgttaa ttttcatatt caattgtgaa ctcaatggct tcatcaggaa agatttcttt
     1621 taacttatcg aaaacatagt cttttttatg atataaaaaa acatg
//
LOCUS       DAZKIV010000331         1664 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZKIV010000331 DAZKIV010000000
VERSION     DAZKIV010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.333"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..705)
                     /locus_tag="ACHOT6_004941"
     CDS             complement(<1..705)
                     /locus_tag="ACHOT6_004941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949973.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIEMVTLLNALQPGGKCVITGDFQKELAYLQNVILYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNVVFSCGTGGIVDLSSLDLRN
                     IDLELYDFTDKHMANAILNPFKLDDTDFTNANMFQVNFVSSKQNTTISWDYLLKITPV
                     LTSISDMYSEEKIKLVE"
     gene            complement(727..1265)
                     /gene="yhaB"
                     /locus_tag="ACHOT6_004942"
                     /pseudo
     CDS             complement(727..1265)
                     /gene="yhaB"
                     /locus_tag="ACHOT6_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
     gene            complement(1521..>1664)
                     /gene="tdcR"
                     /locus_tag="ACHOT6_004943"
     CDS             complement(1521..>1664)
                     /gene="tdcR"
                     /locus_tag="ACHOT6_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator TdcR"
                     /protein_id="HFP9949974.1"
                     /translation="RYLLENKYLFYQYFKISNTGIDHLEQLINVNFFSSDRTSFCECN
                     RFP"
BASE COUNT          524 a          285 c          298 g          557 t
ORIGIN      
        1 ttcgacaagc ttaatttttt cttcagaata catatcgcta atgcttgtta gaacaggcgt
       61 tatttttagt aaataatccc aggagattgt ggtgttttgt tttgaactaa cgaaatttac
      121 ctgaaacatg ttggcattag taaaatctgt gtcatccaat ttaaaaggat ttaaaattgc
      181 attggccata tgtttatctg taaaatcata aagttctaaa tcgatattcc gtaagtccag
      241 agaggataga tcaactatac ctccagtccc acaactaaaa actacgttca acctcctgat
      301 ttcattgtaa atgtctttca atgggaaatt aacattgata ttgcctgtgg atatttcctg
      361 gttttttaac ggttcattaa ttgtaagttc acaggtattg tctgatcgtt gaataattag
      421 ggcgttataa ccaaaaaaat ccatacgaag agacgaatca ttataaagaa ttacattttg
      481 taagtacgct aattcttttt gaaaatcacc agtgataaca catttcccac ctggttgtaa
      541 cgcgttaagt aaagtgacca tttcaataga ggtctgtttt agtttttcta tacctggtgc
      601 tagtgcttcg aggttattaa ctatggggct ttcaggaaga tcattcattt ttcgacggac
      661 taaatcacta ctaatatcat taccaattga tgaaacaggg aacattattt tatcctttat
      721 aaataatcaa taaaaatcat tattaaaatg atcgggtgaa aaaaagagaa tttgccaata
      781 atctgaagta atgcaggttt tattaaagtg cgtagggggg gttaagatgg cttctttttg
      841 ggtgaacttt gcgcaatact tagaaatcgt ctccttaata cattccagct ctttttattg
      901 acggctgagc aagtacattg ttcaccaaaa ggtgcaaaag tgcctacatc taatgtttgt
      961 atgatataat catcatgttc tcctgtagaa atatctatga tgaatggact catattttcc
     1021 ggttggattc ggatcatgac agaaggaaca tcgccttgat agaaattcaa tgcagtaatt
     1081 gcagataata ctctccgcag ttcattactt atactgtaaa ttttattaac gtcattggtg
     1141 gagacaattt ctgcaaactt tctttttacc gtccaataat caatatttct attgtgattg
     1201 ttaaccactg cgttcattgg cgggattgaa gggtgaaaaa tatgtgcaat tgggaagcct
     1261 ttcattgatg cctgccttgt cgtcatgaca aacaataata gatgcttatt ttcctcgcag
     1321 ggttctttcg cactatcaaa aaacgctcat ttacccggcg ttacagtaaa cgaagcaaca
     1381 ttctgtggcc tacgacgatc aaaagctctg tgaaaatttc ccttgttgtc tttgtctgac
     1441 cttatcgcgg cgatcagcaa ttattcgttt tgtgatcctc aacacaaaat gctattcgtc
     1501 gcctatgagt ggagaagaat ttatggaaaa cgattgcatt cacaaaatga cgtcctgtca
     1561 gaagagaaaa agttgacgtt aatcaattgt tcgagatgat caatacctgt attcgaaatt
     1621 ttaaaatatt gataaaataa atatttgttc tccaacaaat agcg
//
LOCUS       DAZKIV010000332         1630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZKIV010000332 DAZKIV010000000
VERSION     DAZKIV010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.334"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..443
                     /locus_tag="ACHOT6_004944"
     CDS             <1..443
                     /locus_tag="ACHOT6_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9949975.1"
                     /translation="ETSPHPDPYISSLIYNALPLTDAHITPPGESSLSDLSEAEEAQK
                     QPKGHKQPSFDVLDDWDAPLPWETEETPVSSSEKVANDNEEDVLENFASLICQQKHTR
                     TGGSSFIEFANSLACRYGLKRPNGIEAYFSKHYPNVYEDFDIPF"
     gene            460..738
                     /locus_tag="ACHOT6_004945"
     CDS             460..738
                     /locus_tag="ACHOT6_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949976.1"
                     /translation="MIQYLIKSKVDRIQCNDTGKRIYETLAYLYKGKPTPLKYSDVLH
                     RAGCSEDGLKFWLKQLSNFGVIEIKELSFSTFNLKRLDKEIVFIYSTL"
     gene            868..1278
                     /locus_tag="ACHOT6_004946"
     CDS             868..1278
                     /locus_tag="ACHOT6_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949977.1"
                     /translation="MAKRKNNVVKKIGDSASLLSKPKSILRRKDFKELVTLTKQNSAP
                     GEWKTEIIEHSPSVPCGDEFNALQEILSSTPGVFWKPRKRKEYIVDSSDLRKYQILGF
                     EDYNHYVGYLATNGLNNLVPEFQILDNADHYGDF"
     gene            1280..1615
                     /locus_tag="ACHOT6_004947"
     CDS             1280..1615
                     /locus_tag="ACHOT6_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949978.1"
                     /translation="MNKNTYDTIYSLINYYEDDYLLPLNRAELEAYKENTPAALNEAF
                     KHWDLAVNAFEHLSKRVEMLCKRENAYLTADQVWELSNWIEDIESDVRYVGDGLVELA
                     QRLGAITEE"
BASE COUNT          565 a          312 c          305 g          448 t
ORIGIN      
        1 tagaaacatc cccacatcct gatccgtata tttcatcttt gatttacaat gctctccctc
       61 tcacagatgc acatattact cccccaggag aatcatcact ctctgatctt tctgaggcag
      121 aagaagcaca gaaacaacct aaaggccata aacagccttc ttttgatgtt cttgatgatt
      181 gggatgcgcc tttgccgtgg gagacggaag aaacacctgt ttcatcaagt gaaaaggtag
      241 ctaatgataa tgaggaggat gtattagaga attttgcctc tcttatctgc cagcagaaac
      301 acaccagaac aggaggatca tcatttatag aatttgcaaa ttcactagct tgtagatacg
      361 ggctaaaacg accaaatggt attgaggcat atttttctaa gcattaccca aatgtatacg
      421 aagactttga tattcccttt taaaaagtaa ggaggactat tgatacagta tttaataaaa
      481 agcaaagtag acagaattca gtgtaacgac acaggaaaac gcatctacga aacgcttgct
      541 tacctctata aaggaaagcc aacaccgcta aaatatagcg atgtgttaca ccgagcaggg
      601 tgctctgaag atggtttaaa attctggctg aaacagctat caaacttcgg tgttatagag
      661 attaaagaat tatctttctc tacattcaat ctcaaaagac tggataaaga aatagtattc
      721 atctattcca ctctctaaaa tctctctatg taattaaaat ataaaaggaa aacattacta
      781 tgatagtagc tatgtattgg ttattgttct ttctcacgtt agaaacatat ttcggggtgt
      841 tttcctttaa aagaaaagga aaaataaatg gcaaaaagaa aaaacaacgt tgttaagaaa
      901 atcggtgatt cagcttctct tctaagtaaa cctaaatcta ttctaagacg taaagacttt
      961 aaagagctag ttacgctcac caagcaaaat agcgcccctg gcgaatggaa aacagagatc
     1021 atagaacatt ctccctctgt gccttgcgga gatgaattta acgccttgca agaaatctta
     1081 tcttcaacac ctggcgtatt ctggaaacct agaaaaagaa aagagtatat cgttgatagc
     1141 tctgatttgc gcaaatacca gattttagga tttgaagact ataaccatta tgttggttat
     1201 ctcgccacca atggcctaaa taatttagtt cctgaattcc aaattctaga taatgctgat
     1261 cactatggag acttttaata tgaacaaaaa cacgtatgac acaatttact cactaattaa
     1321 ttattatgag gatgattacc ttctgccttt aaaccgcgct gaacttgaag catacaaaga
     1381 aaacacgcca gcggcactaa atgaggcgtt taaacactgg gatttagctg tgaatgcctt
     1441 tgagcacctc tctaagcgtg tagagatgct ctgtaagcgt gagaacgcat acctgacagc
     1501 ggaccaggta tgggagctat caaactggat agaagatatt gaatctgatg ttcgctatgt
     1561 aggagatgga cttgtcgaac tagctcaacg cttaggcgct atcacagaag aataaaaaaa
     1621 agcaacaaaa
//
LOCUS       DAZKIV010000333         1611 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZKIV010000333 DAZKIV010000000
VERSION     DAZKIV010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.335"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            246..1604
                     /locus_tag="ACHOT6_004948"
     CDS             246..1604
                     /locus_tag="ACHOT6_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFP9949979.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFG
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDIWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
BASE COUNT          466 a          252 c          348 g          545 t
ORIGIN      
        1 ttaattgtta ttcctaatgt atctatgaat taatgtttta taaggatttt ctataaccat
       61 ttataagtat tttcaagcct ggcttgttgc aaacaatgta taaagcactt aggcaataat
      121 aattacattc agcaactatc atcatcggta ttgtttgtgg gcggaatttc atattgacaa
      181 cagtacagtt cttatatcta ttaataatag aaagggatct acaacctaca gattggtgta
      241 gctttatgga aaaagactat ttgagaatta gtagtactgt attagtgagc ttattgtttg
      301 ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag gaagttgttc
      361 cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact gttgcattta
      421 tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt atacttagtt
      481 tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt cagcaaatta
      541 ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat tgcaatctat tatttgtttc
      601 gtcagatgag tttgtgcata ttaatatttc tggcattggt gaataaagtt agtgaaaaca
      661 caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg ttttttgttt
      721 ttggggggcc gatagttgct cacatacttt ctagtcatta tgagagctac aatttacaca
      781 ttgccgagtt aaccaatgaa aatggtcagg tggtttggaa agcctcatat gttaccataa
      841 tgattttcat gtggttaacc ttgctttcgg ttaatctata ctttaatggt ttacgctatg
      901 atatctggaa tggagtgacg gtgattgctt tttgtgccgt actgtacaat atctctctgt
      961 tatttatgag taggtatagc gtgtccactt ggtatattag tcgcactatt gaagtagtca
     1021 gtaagttaac cgttatggta atatttatgt gccatatatt cagtgcgcta cgagtaacaa
     1081 agaacattgc acatcgcgat cccttaacca atatatttaa cagaaattat ttttttaatg
     1141 aactgacagt tcaatcagca tcagccaaaa aaacgcctta ttgcgtcatg attatggata
     1201 tcgaccactt caaaaaagtc aacgacatct gggggcatcc ggttggcgat caggtgataa
     1261 aaacagtggt gaatatcatt gggaaaagca tacgaccaga tgacctttta gcgcgcgtcg
     1321 gcggcgaaga gtttggcgtc ttgctgacgg acatcgatac tgaacgtgcg aaagctttag
     1381 cggaaaggat tcgggaaaat gttgagcgtt taactggcga taatcctgaa tacgctatac
     1441 cacaaaaagt gacgattagt attggcgctg ttgtgactca ggaaaatgcg ttaaacccga
     1501 atgagatcta tcgactggct gataacgcac tttacgaggc gaaagagacc gggcgtaata
     1561 aggtggttgt gagggatgtg gtgaattttt gtgagtcacc ataaagcggc a
//
LOCUS       DAZKIV010000334         1607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZKIV010000334 DAZKIV010000000
VERSION     DAZKIV010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.336"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..149)
                     /locus_tag="ACHOT6_004949"
     CDS             complement(<1..149)
                     /locus_tag="ACHOT6_004949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9949980.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDVRKTASAV
                     EQGLSR"
     gene            complement(130..543)
                     /locus_tag="ACHOT6_004950"
     CDS             complement(130..543)
                     /locus_tag="ACHOT6_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9949981.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWHRFYRTHYFQDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGIPGGVRYVPAGW"
     gene            complement(570..1001)
                     /locus_tag="ACHOT6_004951"
     CDS             complement(570..1001)
                     /locus_tag="ACHOT6_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9949982.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTQSPSMNEAVMKIEQEVLTTWPADAIARAED
                     VVLCLSGMIEAVRPDTDITEVAKNNTLTDDDFSAGKSSTAS"
     gene            complement(1015..>1607)
                     /locus_tag="ACHOT6_004952"
     CDS             complement(1015..>1607)
                     /locus_tag="ACHOT6_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9949983.1"
                     /translation="YDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIG
                     WFESGDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERS
                     KITPVSAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTI
                     TVNGVATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
BASE COUNT          353 a          503 c          371 g          380 t
ORIGIN      
        1 cggctcaggc cctgttcaac agcactggcg gtttttctga cgtcggtatc cagacctgaa
       61 aaatgacgcc ttacccggct catctgctca tcgaaacgga ccgcatccag actcaggtca
      121 ataacaagat caccaaccgg ctgggacata tctcacacct cccggaatcc ccgctgaagc
      181 catcattaat gcggcatcat ccaccatgac atccgccaca tccgcagacg ataaaatatc
      241 gcgccctccg tccccaccga accggacgcc tccggcaagt cctgccgctt tctgcatcag
      301 cattttgtcc tcatccggcc tctccacctg ctcttcctca tgccggggga caagcagact
      361 gaaatcagag ggatgcatat ccggatcgca aaaaaacagg ctgagtacag cgtacgtcag
      421 cccggaaaaa tgcatatcca gctgggtatc ctgaaaataa tgcgtgcggt aaaaacggtg
      481 ccagtcggca tattcggtgg atgtcatccc ggcaagcatg gcgcgccagt cgggtctccc
      541 catctcacgc gccagtctga gggtaaagtt cagctcgccg tcgaagactt tcccgcagaa
      601 aaatcatcat cagtcagcgt gttatttttc gccacttcag taatatcagt atccggacga
      661 acagcttcga tcatcccgga caggcacaac accacgtctt ccgcccgggc aatggcatcg
      721 gcaggccagg tggtgagcac ttcctgctct atcttcatca cggcctcatt cattgacggt
      781 gactgcgttt tctgtggatg gttatgccac agggacatcg ccaccagaaa cgcgccggtt
      841 ctgacgagat cttccacgct tacctgcagg ttgccgctgg attctgcctg ttctgcacgc
      901 cgtttcagga gggcaagatg ctcgatacgc tgcagcgcag acaattcgga aagcgtgaca
      961 gacacaccgt tatattcaaa ttgttctgtt ttcagaaaca tgtatgacct ccgtttaccc
     1021 tgcagcgccc gcttcagtaa cggtgacttc agccactgcg gcgaactgac catttccgct
     1081 caccacaggg atctgcacct tacctgtcgc cacgccgttt accgtaattg tcatatcttt
     1141 cacactaatg gtggctttcg acggatcggc ggaaaccgct ctgaacgtct tgtctgttgc
     1201 actttccggc tcaaaagaaa ccgtcagggt ggttgttttc ccttttgcca ccgtaccgga
     1261 tgtcggcgtc accttaatcg cactgaccgg cgtaattttg ctgcgttctt ccgctacaga
     1321 aggtttaccc acgttagtga ctttcaccgt gcgggtgatc acttctttcg ccgtcacggc
     1381 cttaccgata ctgctgaccc agccacgaaa cacatccacc gtgccattcg gaaaacggat
     1441 tttataggcc cggacatcgc cgctttcaaa ccagcctata agccctttct ggccttcctc
     1501 tcccggtttc caggccagcg taaaactggt atcacctgca gatttctgcc cctgcccggt
     1561 cgcggtccag tccgcgtctt catcatccag gtagttatca tcgtagg
//
LOCUS       DAZKIV010000335         1607 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZKIV010000335 DAZKIV010000000
VERSION     DAZKIV010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.337"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1247
                     /gene="ygeH"
                     /locus_tag="ACHOT6_004953"
     CDS             <1..1247
                     /gene="ygeH"
                     /locus_tag="ACHOT6_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFP9949984.1"
                     /translation="ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYD
                     RCIETIYRKGYRFSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIIS
                     NKKIDGLYTYPMAATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAK
                     NLFLIASNHLPVDELHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAG
                     KKELYDFTPESIYRAMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAA
                     QKALELLDYVSDITTVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYR
                     ALVHFHNEKIEEARICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETES
                     ESHRVIIDNILKLKQLTRICMR"
     gene            1415..>1607
                     /gene="ygeI"
                     /locus_tag="ACHOT6_004954"
     CDS             1415..>1607
                     /gene="ygeI"
                     /locus_tag="ACHOT6_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFP9949985.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPI"
BASE COUNT          584 a          277 c          270 g          476 t
ORIGIN      
        1 ttgaatccgc tggtcatgtt gtactgaaag atatgatcat cgaatcagta tggaaaaata
       61 ttattgttag tgacgagtcc ctgacaagat gtatctattc tttgcgctgc atttttgaaa
      121 aaattggcta tgatcgttgc atagaaacaa tctaccggaa aggttatcgt ttcagcgggc
      181 aggttttcaa aactaaaatt aatgaagata atacttcaga ctattccata gctatattcc
      241 ctttcactac ttcgttgaat acactggatc cattaatact taatcaggaa ttagtgcaaa
      301 tcatttcaaa taaaaaaatc gatggtctct atacctatcc gatggctgcg acaaattttt
      361 gtaatgatca catatctcaa aattcattct tgagcagatt caagccagat tatttcgtta
      421 caggaagaat aaaccagaat aatgcagtga acactttata cattgagttg atcgacgcta
      481 aaaacctttt cctcatcgcc agtaatcatc tccctgttga tgaactacat aatacatcac
      541 aatttattat agataatatc cttcaaacgg tacataaacc agaacgatct gtaagattag
      601 ctaagcagga ccaaggatat aagaatcatt atttatcaga tgaaatgtta gccggaaaga
      661 aagaacttta cgacttcacc cctgaaagca tttacagggc catgactata tttgatagat
      721 tacaaaataa aagtgatata cagacgctaa aaacagaatg ttattgcctt ctagcggaat
      781 gccatatgtc tttggcactt catggaaaaa gtgaacttga acttgctgct caaaaagcat
      841 tagagctttt agattatgta tcagacataa ccactgtcga tggaaaaatt ttagctatta
      901 tgggactgat aactggtctg tctggacaag caaaagtatc tcatatctta tttgaacagg
      961 ctaagataca ctcaactgat atagcctctc tctactacta tagggcacta gtccactttc
     1021 ataatgaaaa aattgaagag gcaaggattt gtatagacaa atcactacaa ctcgaaccca
     1081 gaagacgaaa agcagttgtg ataaaagaat gtgtagatat gtatgtgcct aacccgctca
     1141 aaaacaacat taaactctac tataaagaaa ctgagagtga aagccatcga gttataattg
     1201 acaacatttt gaaattaaag cagctgacga gaatttgtat gcgataaatt tttattgacg
     1261 caattttatt atttagatgt atgcaacttg ttatttaaac aacagagata aaaatatcta
     1321 agatattcac cttattgcaa gatatttaaa atgctctaga gttaaaagta tgatgtaaag
     1381 accattggcc tggacaatta aaaaagggag ctttatgact aatccaatcg gtatcaataa
     1441 tttatctcaa agttcaaata tagcgaatgc aacgggtgat gaagtcgtat cattagataa
     1501 acacatcaac accagtgcta ctgatacaga tcaaatacaa gcatttatag tttcaacatg
     1561 gatggcgcct tttcaaaatg atatgtattc agaagataat cctatct
//
LOCUS       DAZKIV010000336         1605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZKIV010000336 DAZKIV010000000
VERSION     DAZKIV010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.338"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(118..471)
                     /locus_tag="ACHOT6_004955"
     CDS             complement(118..471)
                     /locus_tag="ACHOT6_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949986.1"
                     /translation="MRQVLNINVGLGSDIPAGHSPSIFEACLHAITASKALNDGGNSQ
                     LSSEVWGYSGASVSFREAFWLATGGDGKILDLPIGKLRKDYFVDTIVIDTNGHNLDII
                     IYDDTTEDILQKLII"
     gene            695..874
                     /locus_tag="ACHOT6_004956"
     CDS             695..874
                     /locus_tag="ACHOT6_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9949987.1"
                     /translation="MGNILFDEKNNRFWPIDFSNSYEHYYSIDKDSKLFVNEMADREF
                     CSILKTLRENAISNI"
BASE COUNT          500 a          323 c          317 g          465 t
ORIGIN      
        1 ctaaaaacat tgttattgat aaactgtcag agaaggattt ctacccttct ctgactacct
       61 ctttgataaa tattctcacc attaacacat accttgcatg tattattacg ctgcgtgtta
      121 tattattaac ttctgcagaa tgtcctcagt tgtatcatca tatattatga tatctaaatt
      181 atgaccatta gtatcaatta ctatagtatc aacaaaataa tctttccgaa gttttcctat
      241 tggtaaatcc agaatttttc catcaccgcc tgttgcaagc cagaaagcct ccctgaatga
      301 aacagatgct ccggagtatc cccagacctc agaagataac tgagagttgc ccccatcgtt
      361 aagggcttta cttgctgtta ttgcatggag acacgcctca aatatcgatg ggctatgtcc
      421 tgctgggata tcactcccaa gaccaacatt tatattcaga acctgacgca agataaatat
      481 atctgtttgc aaaaatagaa attagaaaga ggactgtgag aaattccggc agatgctgag
      541 tctataagag gcatatgatc atcactgata tgattgcggt atgtaagaat ggtttttcgt
      601 gtcatcagat cataattata taatgcctga ctatcggtaa caccatatct ctcaacgaca
      661 acgtcacagt gtaattattc atggatactt acatatggga aatatcttat ttgatgaaaa
      721 gaataatcgt ttttggccga ttgatttttc aaacagttac gagcattatt attcaattga
      781 caaagatagc aaattatttg taaatgagat ggcagacaga gagttttgct caatattaaa
      841 aactttgcgg gaaaatgcca taagcaatat atagaaaaat gatattgctg gcattgttca
      901 ctgatattct cgcggcacat ttcaactaaa actcaggaga gaaggagtga atgttgctca
      961 tatattgaga gtccaagagg tcgtccacct accaaaacgg ccaacaatct tggtgtgggc
     1021 agtagcttgt gataacactt tattaacatt gagcgctttt aggatatatc tcctgagtat
     1081 tagcataaac ttctaatata acttaataat ttgagatcaa atatgtattt catcggttat
     1141 catggcacga gtgaggcgtc agcccagaat attctttccg tcggagttca ggagcgcttc
     1201 cttcctccaa cgggacaaat cggcagaggt ttctacattg cgaagatgaa cggtacgcta
     1261 ccacagtggg gcgctgaaaa cgcaaccgaa ttagcccgca gaaatcagcc gtatttcact
     1321 cgcatggtca gcttattgac cggcgagtca aataatccat ggcttgataa agctgctcag
     1381 cgaacaattt taaaaattta cactgtccgt ccgctattgc attgtaaatg gaacatcatg
     1441 aaccccggcg acttagaatt cttgaaggcc acactctatg acaacggtgg cccaggtctg
     1501 gatcttaagc gcgattgcaa atggttgtac caccagagga actccagtac ctgcgggtga
     1561 agagagatga tggcatcttt gaaaagccgc agaactggtt ttcga
//
LOCUS       DAZKIV010000337         1590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZKIV010000337 DAZKIV010000000
VERSION     DAZKIV010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.339"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1590
                     /locus_tag="ACHOT6_004957"
                     /pseudo
     CDS             1..1590
                     /locus_tag="ACHOT6_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
BASE COUNT          507 a          381 c          406 g          296 t
ORIGIN      
        1 gaaaacatca ccgttgatat ggttattagc gcacaggaat tgttgcagga agacatggca
       61 acattcgacg gacatatcgt tgaagcgttg atgaaaatgc cagatgttaa cgccatgtat
      121 ccggagctta agctgcatgc catcgggtgg gttaagcata aatgtaagcc tggtgccaaa
      181 tggcccgaaa ttcaggcaga gatgcgcatc tggaaaaaac gtcgcgaagg tgaacgcaag
      241 gaaaccggaa aatacacgtc tgttgttgat ctcgcccgcg ccagagccaa tcaacagtac
      301 actgaaaatt caacaggaaa aatcagcccg gtcattgctg ccattcatcg cgaatacaag
      361 cagacatgga aaacactgga tgacgaactg gcctacgctc tctggcctgg tgatgtggat
      421 gccggaaaca ttgacggcag catccatcgc tgggcaaaaa atgaagttat cgacaacggc
      481 cgcgaagact ggaagcgtat ctcggcatca atgcgcaaac agcctgatgc ccttcgctac
      541 gaccgccaga ctatttttgg ccttgtccgt gaacgtccga tcgacattca caaagatccc
      601 gtagcactga acaaatatat ctgcgaatac ctgacgacaa agggcgtgtt tgagaatgaa
      661 gaaacagacc tgggcactgt tgatgttctc cagtcatcag aaacacaaac tgatgcagtg
      721 gaaactgagg tatctgatat cccaaaaaat gaaaccgcgc cggaagctga accatctgta
      781 gagcgtgagg ggccgttcta cttcctcttc accgacaagg atggcgaaaa atacggtcgc
      841 gcaaacaaac tttctggtct ggataaggcg ctgtctgccg gggctactga aatcacgaaa
      901 gaagacatat ttcgcccgca aaaacggtac atactcaggt tcacaacaaa atactggtgc
      961 atctgacacg accgcacaac cagggtcagt aaaagttacc gctgacgaag taaacaaaat
     1021 tatgcaggca gccaatatca gccagcctga cgccgatgaa ctgcttgcag tatcacgtgg
     1081 tgaatttgtt gaagggatta gcgacccgaa tgatccgaaa tgggttaagg ggatccagac
     1141 ccgcgattct gtgaaccaga accagcaaga aacggaacag aacgaccaga aagcggaaca
     1201 aaacagccca aatacgcaac aaaacgagcc agaaacgaaa caacctgaac cagtagtgca
     1261 acaggaaccg gaaaagatct gcaccgcctg cggtcagagc ggtggcggca actgccctga
     1321 ttgtggtgcg gtgatgggtg acgcaacata ccaggaaata ttcgatggag agaatcagcc
     1381 tgaagttcag gaaaatgatc cggaggaaat ggaaggcact gcgcatcagc acaaggagaa
     1441 cactggcggc aatcagcatc atgccagcga tagtgaaact ggcgaggcgt cagatccctt
     1501 aattaaggcg aacggtcatc ataatctcac atccaccagc agagcgggga ttcatctgat
     1561 gatcgacctt gaaaccatgg gaaaaaatcc
//
LOCUS       DAZKIV010000338         1578 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZKIV010000338 DAZKIV010000000
VERSION     DAZKIV010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.340"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            112..630
                     /locus_tag="ACHOT6_004958"
     CDS             112..630
                     /locus_tag="ACHOT6_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFP9949988.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            841..>1578
                     /gene="tssI"
                     /locus_tag="ACHOT6_004959"
     CDS             841..>1578
                     /gene="tssI"
                     /locus_tag="ACHOT6_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP9949989.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLALSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQ"
BASE COUNT          376 a          422 c          415 g          365 t
ORIGIN      
        1 aaaaatactg aaaaatgatg cggttccgtg aagtgagctg catctgcctg tctgggatta
       61 acggcaggtc attgtaagta gtaattaacc acgttaacaa ggagtttaca aatgccaaca
      121 ccgtgttata tctccatcac tggtcagacc cagggaaata ttactgcagg tgcatttacc
      181 gctgattctg tcggcaacat ctatgtgcag gggcacgaag acgagatgct ggtgcaggaa
      241 ttcctgcata acgttactgt cccgaccgat cctcagtccg gtcagcctgc gggtcagcgt
      301 gctcacaagc ctttcatttt taccgtggcg ctgaacaaag cggttccgct gatgtacaac
      361 gcactggcct ccggcgaaat gctgccaacc actgaacttc actggtggcg tacctctgtg
      421 gagggcaaac aggagcatta tttcactacc cgcctgactg attcgacgat tgtcgatatg
      481 aaactgcata tgccacattg ccaggatccg gcgaaacgcg agttcaccca gttgctcgaa
      541 gtttcactgg cctaccgcaa aattgagtgg gaacacgtca aatcaggcac ctctggtgcc
      601 gatgactggc gcgcaccgct ggaagcataa gttaagccaa cagcatccgt ctatcaggcc
      661 ggatgctgtt tttgtattac gacgtaataa agaaaggttg tcagtaaggg gttacgttca
      721 cggaagatgg cggatgccgg aaacttcggc ggtagcgtac ctgaacgcaa gcgtaacact
      781 gtactgtcac agccgcttta acagcgaact gtctgttttt ccttaacccc ggaggttgct
      841 atgtcaaccg gattacgttt cacgctggaa gtggacggcc tgccaccgga cgcgtttgcg
      901 gtggtctcct ttcatttgaa ccagtcactc tcttcgctct tttccctcgc tctctctctg
      961 gtcagccagc agtttctctc ccttgaattt cagcaaatac tggacaaaat ggcctacctg
     1021 acgatatggc agggcgatga cgtacagcgc cgggtgaaag gtgtggtgac ctggtttgaa
     1081 ctgggggaga acgacaaaaa ccagatgctg tacagcatga aggtgtgccc gccgctgtgg
     1141 cgcacagggc tgcgccagaa cttccgtatc ttccagaatg aggacatcga aagcatcctc
     1201 gcgacgatcc tgaaagaaaa cggtgtgacc gagtggagtc cgctgttcag cgagccgcat
     1261 ccttcccgtg agttttgtgt ccagtacggc gaaaccgatt acgatttcct gtgccggatg
     1321 gcggcggagg aaggcatctt cttttatgag gagcacgcgc aaaaaagtac cgaccagagc
     1381 ctggtcctgt gcgacaccgt gcgttatctg ccggagtcct ttgagatccc ctggaatccg
     1441 aacacccgta ccgaagtaag caccctctgc atcagccagt ttcgctacag cgcacaaatc
     1501 cgcccttctt ccgtggtgac caaagactac acctttaaac gccccggctg ggccggacgt
     1561 tttgatcagg aaggccag
//
LOCUS       DAZKIV010000339         1568 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZKIV010000339 DAZKIV010000000
VERSION     DAZKIV010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.341"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..548)
                     /locus_tag="ACHOT6_004960"
     CDS             complement(<1..548)
                     /locus_tag="ACHOT6_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFP9949990.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGA"
     gene            817..933
                     /locus_tag="ACHOT6_004961"
                     /pseudo
     CDS             817..933
                     /locus_tag="ACHOT6_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1041..1114)
                     /locus_tag="ACHOT6_004962"
     tRNA            complement(1041..1114)
                     /locus_tag="ACHOT6_004962"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1078..1080),aa:Arg,seq:tct)
     gene            complement(1128..1204)
                     /locus_tag="ACHOT6_004963"
     tRNA            complement(1128..1204)
                     /locus_tag="ACHOT6_004963"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1168..1170),aa:Arg,seq:tcg)
     gene            complement(1212..1287)
                     /locus_tag="ACHOT6_004964"
     tRNA            complement(1212..1287)
                     /locus_tag="ACHOT6_004964"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1252..1254),aa:Ile,seq:cat)
     gene            complement(1316..>1568)
                     /locus_tag="ACHOT6_004965"
     CDS             complement(1316..>1568)
                     /locus_tag="ACHOT6_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9949991.1"
                     /translation="ALRLHMSESLLKIKLKNEGHMFSRLLLEERMRVAVNMLCSRHGY
                     GQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          394 a          426 c          389 g          359 t
ORIGIN      
        1 gcgccctggg tgaatgcaga accaccacga cagcatggta ccagcaggat ccccgcgtta
       61 ttcgggatat acggaagcag ttttttggca atatgtaagc cctggccgac acagccgtac
      121 tgccctttgc tcaggtctgc cttcggatga ttcagcgtac tcatatcctg cacatcatgc
      181 aggcagtggt cagccggaat gatgtcgtta tacgtacaac tctctccacc cggagttacc
      241 gtgctgcggc gcgccagctg tttaatgcgc ggatccggag catcgtaaga atccggtaac
      301 ggaagccctt caccgtaggc catgccgttg gactgtccgg caagcacaac cacgtagaac
      361 cagtccggct cagatgaagg gccgacctgt ggatctcctt caatagccac cgcctgcatc
      421 agtgtgtacg gcgtaatggc aaccggtccg ccgtatggct gccagccctc tttcagtttg
      481 tgtgtcagct tttccgcaag gtctgacggc gaagccgccc tgacaacatc atagtgttta
      541 aatgccatga atcctcccgg ccgggataat attgtgagta aaatgaggag cgggctgaag
      601 tccggaagtt acaggacaat ggcagaagag agacaacagc ccgcaatacg aaaaaggccg
      661 cgctattgcg cagagtgatt actgtcggat attattcgcc agctgaaata ttacttcacg
      721 ttttgttgtt tattccttgc cgcccgcgtc tccctgcgcg ggcttttttt gtccataaga
      781 aagcccctcc ggagaggggc tggagagtgg cgctatgtgc cattgcatgg tgccgggtgc
      841 ctcccggtga attcagtacc agcacctgaa tccgcgatta tcccatatac ctactcgctg
      901 attgcccctc cgcacagggg gattcaccat gccagtttct tttaacaaac tccccgcaaa
      961 ccagacaaca gtcaaccgcc tgaattgtga gacatttaaa aaaaaggccc gcaaaagcga
     1021 gccagggaaa ataagtgtgc cgcgttgtac tggattcgaa ccagtgaccg attgcttaga
     1081 aggcaattgc tctgtccggc tgagctaaca acgcaggata cagataatgg accgccttcg
     1141 gggacccgaa ctccgcgcaa ccagcttcga aagctggcgc tctttcctga tgagctaatg
     1201 gcggtatgtg atggtggccc ttgctggatt tgaaccagcg acctggcgat tatgagtcgc
     1261 tcgctctcac cactgagcta aagggccggg tcaaaaaata ataatcagat gaaaatcaat
     1321 aatcaagccc ttgcctggat acatatctgt ctggcgggaa gccataatag cggtgaaata
     1381 cagaaataaa gtaggaccag cttgaataac cgcatttttc tgctacagcc tgtccatatc
     1441 catgccggga acataacata ttcacagcaa cacgcatccg ctcttccagc aacaagcgac
     1501 tgaacatgtg cccttcattt ttcagtttta tctttaacaa actctcactc atatgcaggc
     1561 gtaacgca
//
LOCUS       DAZKIV010000340         1559 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZKIV010000340 DAZKIV010000000
VERSION     DAZKIV010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.342"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1559
                     /locus_tag="ACHOT6_004966"
     rRNA            <1..>1559
                     /locus_tag="ACHOT6_004966"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          430 a          334 c          491 g          304 t
ORIGIN      
        1 tgtgtgttag tggaagcgtc tggaaaggcg cgcgatacag ggtgacagcc ccgtacacaa
       61 aaatgcacat gctgtgagct cgatgagtag ggcgggacac gtggtatcct gtctgaatat
      121 ggggggacca tcctccaagg ctaaatactc ctgactgacc gatagtgaac cagtaccgtg
      181 agggaaaggc gaaaagaacc ccggcgaggg gagtgaaaaa gaacctgaaa ccgtgtacgt
      241 acaagcagtg ggagcacaga gcaatctgtg tgactgcgta ccttttgtat aatgggtcag
      301 cgacttatat tctgtagcaa ggttaaccga ataggggagc cgaagggaaa ccgagtctta
      361 actgggcgtt aagttgcagg gtatagaccc gaaacccggt gatctagcca tgggcaggtt
      421 gaaggttggg taacactaac tggaggaccg aaccgactaa tgttgaaaaa ttagcggatg
      481 acttgtggct gggggtgaaa ggccaatcaa accgggagat agctggttct ccccgaaagc
      541 tatttaggta gcgcctcgtg aattcatctc cgggggtaga gcactgtttc ggcaaggggg
      601 tcatcccgac ttaccaaccc gatgcaaact gcgaataccg gagaatgtta tcacgggaga
      661 cacacggcgg gtgctaacgt ccgtcgtgaa gagggaaaca acccagaccg ccagctaagg
      721 tcccaaagtc atggttaagt gggaaacgat gtgggaaggc ccagacagcc aggatgttgg
      781 cttagaagca gccatcattt aaagaaagcg taatagctca ctggtcgagt cggcctgcgc
      841 ggaagatgta acggggctaa accatgcacc gaagctgcgg cagcgacact atgtgttgtt
      901 gggtagggga gcgttctgta agcctgtgaa ggtgtgctgt gaggcatgct ggaggtatca
      961 gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct cgccggaaga
     1021 ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa ggcgaggccg
     1081 aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac tgcgaagggg
     1141 ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt gtaggctggt
     1201 tttccaggca aatccggaaa atcaaggctg aggcgtgatg acgaggcact acggtgctga
     1261 agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat caaatcgtac
     1321 cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga actcgggtga
     1381 aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat gtaggtgaag
     1441 cgacttgctc gtggagctga aatcagtcga agataccagc tggctgcaac tgtttattaa
     1501 aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct gcccggtgc
//
LOCUS       DAZKIV010000341         1550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZKIV010000341 DAZKIV010000000
VERSION     DAZKIV010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.343"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            50..1456
                     /gene="gndA"
                     /locus_tag="ACHOT6_004967"
     CDS             50..1456
                     /gene="gndA"
                     /locus_tag="ACHOT6_004967"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFP9949992.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKNEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFSAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
BASE COUNT          390 a          380 c          391 g          389 t
ORIGIN      
        1 gttattaact taccgcatat catgtggtac cccctgacag gagtaaacaa tgtcaaagca
       61 acagatcggc gtcgtcggta tggcagtgat ggggcgcaac cttgcgctca acatcgaaag
      121 ccgtggttat accgtctcta ttttcaaccg ttcccgtgag aaaacggaag aagtgattgc
      181 cgaaaatccg ggcaagaaac tggttcctta ctatacggtg aaagagtttg ttgaatctct
      241 ggaaacgcct cgtcgcatcc tgttaatggt gaaagcaggt gcaggcacgg atgctgctat
      301 tgattccctc aagccatacc tcgataaagg tgacatcatc attgatggtg gtaatacctt
      361 cttccaggac accattcgtc gtaaccgtga gctttctgcc gaaggcttta attttatcgg
      421 taccggtgtc tccggtggtg aagaaggcgc gctgaaaggt ccttccatta tgcctggtgg
      481 gcagaaagaa gcctatgaac tggttgctcc gatcctgacc aaaatcgccg cagtggctga
      541 agacggcgag ccatgcgtta cctatattgg tgccgatggt gcaggtcact atgtgaagat
      601 ggttcacaac ggtattgaat acggcgatat gcagctgatt gctgaagcct attctctgct
      661 taaaggtggc ctgaatcttt ccaacgaaga actggcgcag acgtttaccg agtggaataa
      721 cggtgaactg agcagctacc tgatcgacat caccaaagac atcttcacca aaaaaaatga
      781 agacggtaac tacctggttg atgtgattct ggatgaagca gcaaacaaag gtaccggtaa
      841 atggaccagc cagagcgcgc tggatctcgg cgaaccgctg tcgctgatta ccgagtctgt
      901 gtttgcacgt tatatctctt ctctgaaaga tcagcgtgtt gccgcatcta aagttctctc
      961 tggcccacaa gcgcagccag ctggcgacaa ggctgagttc atcgaaaaag ttcgccgtgc
     1021 actgtatctg ggcaaaatcg tttcttacgc tcagggcttc tctcagctac gcgccgcgtc
     1081 tgaagagtac aactgggatc tgaactacgg cgaaatcgcg aagattttcc gtgctggctg
     1141 catcatccgt gcgcagttcc tgcagaaaat caccgatgcc tatgccgaaa atccgcagat
     1201 cgctaacctg ctgctggctc cgtacttcaa gcaaattgcc gatgactacc agcaggcgct
     1261 gcgtgatgtc gttgcttatg cggtacagaa cggtatcccg gttccgacct tctctgcggc
     1321 agttgcctat tatgacagct accgcgccgc tgttctgcct gcgaacctga tccaggcaca
     1381 gcgtgactat ttcggtgcgc atacttataa gcgcattgat aaagaaggtg tgttccatac
     1441 cgaatggctg gattaatctg atttaaatca attaatcaat gcaaggcccg gagaaaccct
     1501 ccgggctttt ttattataca aagcggcagg ttatggcctt tttttataat
//
LOCUS       DAZKIV010000342         1525 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZKIV010000342 DAZKIV010000000
VERSION     DAZKIV010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.344"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..201)
                     /locus_tag="ACHOT6_004968"
     CDS             complement(4..201)
                     /locus_tag="ACHOT6_004968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFP9949993.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRIQS"
     gene            complement(426..980)
                     /locus_tag="ACHOT6_004969"
     CDS             complement(426..980)
                     /locus_tag="ACHOT6_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFP9949994.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(989..1348)
                     /locus_tag="ACHOT6_004970"
     CDS             complement(989..1348)
                     /locus_tag="ACHOT6_004970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFP9949995.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
     gene            complement(1361..>1525)
                     /locus_tag="ACHOT6_004971"
     CDS             complement(1361..>1525)
                     /locus_tag="ACHOT6_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9949996.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          377 a          425 c          307 g          416 t
ORIGIN      
        1 ccttcaggac tgaatgcgtc tgtaacaacc gcactgatag tctgccagac ccgccataac
       61 cagctgggtc agtattaact ggcagcgttc gcgtgaaagg taagtattct gcgcaatctc
      121 cccgactgtc gccggttcgg taacgcttaa ttcattaaac accactctgg cggtttctgt
      181 catatcctgc tgttttagca tgtctttttc ccttttccgg ttaacgtgac ataccaataa
      241 ctcttgtcga aaaagccagc aagttgaaag accggtatta gcaaccacca gcgcgtttaa
      301 cgccccgtgc cgtttttcag tcataaaaaa acccgcaaaa agcgggctct ttcaaatgtc
      361 catgtctgct attcgcctcg cggtacagct ttgcgaagct taccggaatt gaagcagttt
      421 ttacgtcaaa aagcaataac ttttttctct ataccaaaag ccataaccat tggtttgtac
      481 aaaataaatt ctgccacctt tagccaatgc tcaatgcgtc tttcacaggt tcttaaactc
      541 cattccggat gtgcatcatt cagcaattca gccattttgc gcttagtcat cccccgcccc
      601 acataacgct gactcagaac attgagcagc ccgggataac ctgccagtac ttcaccaata
      661 accctgtcga ttattaacgc ctctgaatcg gtacaatgtg ccagccagct tttttgattg
      721 ccgttgatca tatcccgcaa aaaagcctca agttcaggtt tgtccagacc cgcttttttc
      781 atcctccgga gcgcctcgtt aattgccgtt tttgtcagct ttttagaggt cagtaattgg
      841 ttgaacatat ttcccgtctt accgtcgcca atatacgacc aacgccccca catacgcagt
      901 ttcccctgga tccagacact ttccagcgtt ttcaggcgta aatgctcacc gcttttgcct
      961 gtaatttccg ggtatatcat atttatgctc actcactttc aattttgtaa atcttcacgc
     1021 ccagccgccc accagaaacg agctggccgc gcacaatatt gatttcatca aactgctcgt
     1081 cgtctatgag aagtccggca tgcgtcagcg catccagtgg tgctttcagg atattgtcca
     1141 ggtcacgacg gcgcttatcc ggtggctctg caataatctt tatcgccagc cttccggaca
     1201 ggtttaattt cagccgctgc tggcgaacaa taagtgccac atcccggcga taacgctcac
     1261 cggcttttga tacaaaatat gtgctgccac gacgtcgcca gtaggtgttc accgtcggcg
     1321 ggtaaggcaa aacaaactct atacgcatca gtaacctctt ttacccgagc acgccggttg
     1381 caaaggcgtg atcaagaaaa cgaaaaatta aatcaacctg ggaaccatgc ttttcttcga
     1441 acgccagcgg atccgcatga agctcgttgt gatgctcccg acacagcggt agcgtgaaaa
     1501 tatcgtggga ttttgttccc attcc
//
LOCUS       DAZKIV010000343         1522 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZKIV010000343 DAZKIV010000000
VERSION     DAZKIV010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.345"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..543
                     /locus_tag="ACHOT6_004972"
     CDS             <1..543
                     /locus_tag="ACHOT6_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9949997.1"
                     /translation="KFALALMSGKNKTASAIAAGYSAKTARVKGSQLAKDPEVLAFIA
                     RKQCETVEVDEVPVYRQKKSEQEDKPRRREAAAIPQPDETNPEMPPPVVISPGIEYME
                     DGLPDPVKAMGRLLVENINTDPRLALDAAYKLAQFTHHKKGDAGKKSAKGDAAKKAAN
                     RFAVPPPPRLVVNNDNEGNG"
     gene            540..>1522
                     /locus_tag="ACHOT6_004973"
     CDS             540..>1522
                     /locus_tag="ACHOT6_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9949998.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWTG"
BASE COUNT          357 a          384 c          468 g          313 t
ORIGIN      
        1 aaatttgcgc tggcgctcat gtccgggaaa aacaaaacag cgtcagccat tgccgccggt
       61 tattcggcga agacagccag ggttaaaggc tcgcagctgg caaaagatcc ggaggtgctt
      121 gcgtttatag cccgtaaaca gtgcgagacg gtggaggtgg atgaggttcc tgtttaccgg
      181 cagaaaaaat cagagcagga ggataaaccc cgtcgccgtg aggcggctgc aataccacag
      241 ccggacgaaa caaatccgga gatgccaccg cccgtggtga tatctcctgg tattgagtat
      301 atggaggacg gtcttcccga tccggtgaaa gcgatggggc gtcttctggt ggagaacatt
      361 aataccgacc ccaggctggc gctggatgcg gcttataagc tggcgcagtt cacgcaccac
      421 aaaaaagggg atgccggtaa aaaatcggca aaaggtgacg cggcgaaaaa agcggctaac
      481 cgttttgcgg tgccaccacc accccgactg gtggtgaata atgataatga gggcaacgga
      541 tgatacctgt gtggagcacg gcctgcccgg actgggcaga gcgcctgaaa aaggggctgt
      601 cgattattcc ggccccgatt tatccggatc aggctgcaca tgcactggcg atttttaaac
      661 aactgcggat tgtggatgca ccgggcagcc cgacgttcgg ggagtcctgc gcacagtggg
      721 tgtttgacct ggtggcggcc ctgtttggct cctacgatgc gcagaccggt gttcgccata
      781 tcaaggaagt ttttatcctt atcccgaaaa aaaacagcaa gtccacgctg gctgccggga
      841 tcatgatgac ggcgctgtta ctgaactggc ggcaggcggc gggctacacc attctggccc
      901 cgactgtgga ggtggcggct aacgccttta atcctgcgcg ggatatggtc agacgtgacg
      961 atgatctgga tgacctctgc caggtgcaga cacatatccg gaccatcacc cacagggtga
     1021 cggacactac ccttaaggtg gtggcagccg atccgaatac ggtgtccggt atcaagtccg
     1081 tggggacact gattgatgaa ctgtggctgt ttggcaagca gtacaaagcg gaagacatgt
     1141 tacgtgaagc catcggcggg cttgcctccc gtccggaagg atttgtggtg tacacaacca
     1201 cccagtcgaa tgagccgcca gccggggtgt tcagacagaa actgcagtac gcccgggatg
     1261 tccgtgacgg caaaattcat gatccgcact ttctgccggt gatatttgaa caccctcctg
     1321 aaatggtgga aagcggggct cacctgctga tggaaaacct cgccatggtc aatccgaatc
     1381 tcggttattc ggtggatgag gcttttctgt accgggagta ccgtaaagcc cgggaggctg
     1441 gtgaggaagc atttcgtggc ttcatgtcaa aacatgccaa tgtggaaatt ggtcttgccc
     1501 tgcgttctga ccgctggacg gg
//
LOCUS       DAZKIV010000344         1513 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZKIV010000344 DAZKIV010000000
VERSION     DAZKIV010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.346"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..654)
                     /locus_tag="ACHOT6_004974"
     CDS             complement(<1..654)
                     /locus_tag="ACHOT6_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFP9949999.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     "
     gene            complement(818..913)
                     /gene="ymiC"
                     /locus_tag="ACHOT6_004975"
     CDS             complement(818..913)
                     /gene="ymiC"
                     /locus_tag="ACHOT6_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFP9950000.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1027..1194)
                     /gene="yciX"
                     /locus_tag="ACHOT6_004976"
     CDS             complement(1027..1194)
                     /gene="yciX"
                     /locus_tag="ACHOT6_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFP9950001.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1197..1337)
                     /locus_tag="ACHOT6_004977"
     CDS             complement(1197..1337)
                     /locus_tag="ACHOT6_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFP9950002.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          410 a          362 c          352 g          389 t
ORIGIN      
        1 gatcatagtg gtgtgcgagt cagtaccaac gagtgtatcc ggataagcaa tccattcacc
       61 gtcctgcaat tcactccaca ctgctttgcc gagatattcg aggttaacct gatggcaaat
      121 gcctgtgcct ggcggcacga cgctaaaccg actgaacgcc tgctttcccc atttcaggaa
      181 cacataacgt tcgtggttgc gctccatttc caggcgtacg ttttcttcaa atgcctcatc
      241 atcaccaaaa cgatcgacgg tcaccgagtg gtcaatgacc aggtcgaccg gtgagagcgg
      301 gttaaccttt gcagtatcgc cgccgaggcg tttaaccgct tcgcgcattg cagcgagatc
      361 aacaacggca ggtacgccgg taaagtcctg catcagcacc cttgccgggc ggtaggcaat
      421 ttcacggtca gcatgggcat ttttcagcca tcctgccagc gcgtggatat cctcttcggt
      481 aaccgagtta ccatcctgcc agcgcagcag gttttcgagc aaaactttga gtgacttggg
      541 cagacgggtg atatcgccca gtgatttagc agcaagcggc aggctgtagt agtggtaagt
      601 tttatctttg gcctgcaacg tgtccttact ggcttctcgt agggttgacg acatagctcc
      661 tccttaatga cagggttgcg aagccctgat tctcttcagg gtgaatatta aagataacac
      721 aaacatcgcg taacgatttg ataacaaccc aaattgataa aagaggagaa accttgtggc
      781 aaagcaaatg acaaccccgc cgcagcgggg tcaaggatca gtgagtaatg atccagagga
      841 gttcggccca gaagagcatc gacagagaaa acgcgcccat ccaggaccaa tatttcatat
      901 ttgtgttgat cataggctac acccttaacg gttattattt tgtgttgaga taactcaagc
      961 tgttattgct gtggtgtatt aaaagccctt taattcatta aaaatgggta ataagaatga
     1021 tgataattat tgtttcggct gatcatcttc agcaaacagt tctataaagg cttgctgctg
     1081 cgatgttagt tgccagtcgt ctggaataac gacggaacat tcccgtttcg tttctgtttc
     1141 attgtcacta tgctggcata atggtgacga ctccagttgc tcctgaccga ccatagttat
     1201 ccccacactt tccaaatcag cagtgcgaca accacccaga aaagtgcaga accaagaaaa
     1261 accgccagcc aggctttacg ttttaattca ggatcgcggc ggggttcctg atttccagaa
     1321 ggcattgcta acctcatata atcgccatca cttatcagca agacgttgaa cgattggtgt
     1381 aattaatcat acgatgagac aaatcctaaa gaaactttag tcaaaaatcc agcgaattta
     1441 cgaatcgtct ccagtgaata attcaatctt ttgacctgaa atagatattt gaaaactgga
     1501 gtttgcgagg cgg
//
LOCUS       DAZKIV010000345         1489 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZKIV010000345 DAZKIV010000000
VERSION     DAZKIV010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1489
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.348"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..134)
                     /locus_tag="ACHOT6_004978"
     CDS             complement(<1..134)
                     /locus_tag="ACHOT6_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950003.1"
                     /translation="MGKHHWKVDKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     "
     gene            complement(127..387)
                     /locus_tag="ACHOT6_004979"
     CDS             complement(127..387)
                     /locus_tag="ACHOT6_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9950004.1"
                     /translation="MQSPLRNVRKAHCFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEISEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            485..1177
                     /locus_tag="ACHOT6_004980"
     CDS             485..1177
                     /locus_tag="ACHOT6_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFP9950005.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSALKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQILKRHN"
BASE COUNT          440 a          334 c          315 g          400 t
ORIGIN      
        1 aataatgcgt tctctgttac atccagccag tcagcagctt cagcgtaacc ccccggcaac
       61 gccgcgatag tttttctgac agctttcacg taccactcag gctgtttgtc tactttccag
      121 tgatgcttac ccacggttca cctcctgttc ctgtggttta aacccattct ggttttggct
      181 agattgaaaa cgtgccggat aaagaatctg catttcgctg atttcaccct taaaaaaatt
      241 ggctaaacgt tctgcaagct cgatagatgg aatctgctcc agcctctcaa tacgactcaa
      301 cgtcgctgga ttgacttgaa cacccgcagc aacatgctgc aaagtgaaac aatgcgcctt
      361 acgcacattt cgtaatggtg attgcatatg tcctccaaat attgcgcgtt atgcatatta
      421 tttcacgcaa gtattttgcg caagttgatt tgcttatcac gcaataaaga aatgtaataa
      481 acgcatgaac ataggaaacc gagtcagaca acttcgccaa gcgaagaaca tgaaaatcgc
      541 cgatctcgct gaagcaatag gagtagatgc ggcgaacatc tcgcgcttag aaacgggtaa
      601 gcagaaacaa tttaccgaac aaacactgag taatattgcc aagagcttag gtgttgatat
      661 tgctgatctc tttacctctg ccctcaaaag taatactgta tataaaaaca gtaataatga
      721 ggatgttgcg caggtgaagg atgtgttccg tattgaaatg ctggatatca gtgccagtgc
      781 gggaaatggc cttatccagg gcggtgatgt cattgatgtg attcatgcca tcgaatacag
      841 aactgataat gctgtatcaa tgttcggcgg acgaccagcc aatcacatca aagttatcaa
      901 cgttcgtggg gacagtatgt gtccaaccat tgagccagga gatctcatct tcgtcgatgt
      961 cagcatcaat cagtttgatg gtgatggtat atatgtcttt ggttttgatg acaaaatata
     1021 cgttaaaaga cttcaaatga ttcctgacaa actgctggtg atttctgata accagattta
     1081 ccgtgaatgg ggaattacta gcgaaaacga acaccgattc atggtctttg gaaaggtctt
     1141 aatcagtcag tcgcaaatcc ttaagagaca taattaacct caatatccca tccagcggcc
     1201 accgaaaggt ggctttttat cacccataat ttgcatatct cgcaaaatac tacttgcata
     1261 tctcgcaatt taattttatc ttttgttcca gaccaactac aggattacaa caaaatctgg
     1321 ttgcaacacg gtgcatggtg catgtgtcgt aagcggtcag taaatgtcaa aaacgaacag
     1381 gcaggacgcc cacgaagtag ccgcctgggg catatgaagt ccaggatgat tcgttagcaa
     1441 caaaaaagcg ccctacagga cgcttagctc tttaacaatc tggtcccca
//
LOCUS       DAZKIV010000346         1479 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZKIV010000346 DAZKIV010000000
VERSION     DAZKIV010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.349"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1479
                     /locus_tag="ACHOT6_004981"
     CDS             <1..>1479
                     /locus_tag="ACHOT6_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFP9950006.1"
                     /translation="YSGIWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKW
                     ALYAIGQYCDQTVPDGFGGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTL
                     TFVQDRPSDVVWPYTNSDVVADNEGVGFRYSFSALKDRHTAVEVSYVDPHNGWQTSTE
                     LVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLETQTVDFTLGSQGLRH
                     TPGDIIEICDNDYAGTMTGGRVLSIDAASRTLTLDREVTLPETGAATVNLINGSGKPV
                     SVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSLRRRLFRCVSVRENTDGTFAITAV
                     QHVPEKEAIVDNGARFEPQSGTLNSVIPPAVQHLTVEVSAADGQYLAQVKWDTPRVVK
                     GVRFSLRLTSGSGQDSRLVTTAITADTEHRFSGLPPGEYTLTVRAINSYGQQGEPATT
                     SFRINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSE"
BASE COUNT          301 a          422 c          484 g          272 t
ORIGIN      
        1 tacagcggta tctgggacgg cagcctgaaa ccggcataca gcaataaccc ggcctggtgc
       61 ctgtgggaca tgctgaccca cccgcgctac ggaatgggaa aacgtctggg ggcggcggat
      121 gtggacaagt gggcgctgta tgccatcggg cagtactgcg accagacggt cccggatggt
      181 ttcgggggca cagagccgcg gatgaccttt aatgcgtacc tgtcacaaca gcgtaaggcg
      241 tgggacgttc tcagtgattt ctgctcggcg atgcgctgta tgccggtatg gaacgggcag
      301 acgctgacgt ttgtgcagga ccgtccgtca gatgtggtgt ggccctacac aaacagtgat
      361 gtggtggctg acaatgaagg tgtgggattc cgctacagtt tcagcgccct gaaggaccgt
      421 catactgcgg tggaggtcag ttacgtcgac ccgcataacg gctggcagac ctccacggaa
      481 ctggtggaag acccggaagc catactgcgc tacggacgca acctgctgaa gatggatgcg
      541 ttcggctgca ccagtcgcgg tcaggcccac cgtgccgggc tgtgggtgat aaagaccgga
      601 ctgctggaaa cgcagacggt ggatttcacg ctcgggtcac aggggctgcg tcacacaccc
      661 ggtgacatta ttgaaatctg tgataacgac tatgccggga ccatgaccgg cggacgtgtc
      721 ctgtccatcg atgccgccag ccgcaccctg acgctggacc gtgaggtgac actgccggag
      781 accggtgccg ccacggtgaa cctgattaac ggcagcggta agccggtgag tgtggacatc
      841 accgcacacc ccgcgccgga ccggatacag gtcagcaccc tgcctgatgg tgtggagaca
      901 tacggtgtat ggggactatc cctgccgtca ctgcgccgtc gcctgttccg ctgtgtctcc
      961 gtccgggaaa acacggacgg cacctttgcc atcacggcgg tgcagcacgt accggaaaaa
     1021 gaagccatcg tggataacgg tgcccgcttt gagccgcagt caggcaccct gaacagcgtc
     1081 atccctccgg cagtgcagca cctgacggtg gaggtgagcg cagctgacgg ccagtatctg
     1141 gcgcaggtga aatgggacac gccgcgggtg gtgaagggcg tgcgcttcag tctgcgcctg
     1201 accagtggaa gcggtcagga cagccgtctg gtgaccaccg ccatcaccgc ggatacagag
     1261 caccgtttca gcggtctgcc gccgggggaa tacaccctga cggtcagggc gattaacagt
     1321 tatggccagc agggggaacc ggccaccacc tcgttcagga ttaatgcacc tgcggtaccc
     1381 gccacgattg agctgacacc gggctatttt cagataacag cggtcccgcg tcttgcggtg
     1441 tatgacccga cggtacagtt tgagttctgg ttttcggag
//
LOCUS       DAZKIV010000347         1471 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZKIV010000347 DAZKIV010000000
VERSION     DAZKIV010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1471
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.350"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..658)
                     /locus_tag="ACHOT6_004982"
     CDS             complement(119..658)
                     /locus_tag="ACHOT6_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFP9950007.1"
                     /translation="MSNIGYKRVSTVDQNTDRQLAGVTLDKVFEDKLSGKDTNRPGLQ
                     ACLAYLREGDTLHVHSLDRLGRNTLDILELIKQLNDRGVIVRFHKEGIIADITSAMGK
                     MMLTMLSAVATCERELMLERKREAQAVNPTKRGNGKAVDREGIKKALSAGVSVRKTAE
                     SFNVAPSTVQRIKKEEEEA"
BASE COUNT          430 a          296 c          313 g          432 t
ORIGIN      
        1 taaatacatc taaataccta tatcccttcc cttatggcta gattgcgaga ggtgcttaac
       61 gcgattttca gcgccccccg atctggaaaa tccagaatcc ccaaatatca aggaggcatt
      121 acgcctcctc ttcttccttc ttgatacgtt gcaccgttga aggagcaaca ttaaagctct
      181 cggcggtttt acgcactgac acgccagcgg aaagggcttt ctttatacct tctctatcta
      241 ctgcttttcc attaccacgc tttgtaggat tgacggcttg cgcttctcgt ttacgttcta
      301 acatcagttc acgttcacac gttgccactg ccgacaacat agtaagcatc atttttccca
      361 tcgcgcttgt aatgtcagca atgattcctt ctttatgaaa gcgaacaatt acacctcgat
      421 cattcaattg tttgattaac tccagaatgt ccagcgtatt acgtcctaaa cggtcgagag
      481 agtgcacgtg taaggtgtcg ccttctctca gataggctaa acacgcttgt aagcctgggc
      541 ggttagtgtc tttaccagac agcttatctt caaagacttt atcgagagtt actcctgcaa
      601 gctggcgatc agtgttctga tctacagtgc tcacacgctt ataaccgata ttgctcataa
      661 aagcccctta atgtatcgat ttggcactac ttcaatgcaa atgagtatac cgtattcatt
      721 tggtctgttt caagccttat aggtacggaa tattacacga tttagctgta ttcatttggt
      781 atgcctaatg gatacacagg gggatagatt aaatggtgtt ctgtttatac aggtttattt
      841 gttagatcaa caatttggca tttctaaaca gatgtttgaa cgttgccaaa tgtcttgaca
      901 acacaagaaa agggcttgct atgccacgca ttacacgaaa taaggctgta aaaacaaaat
      961 aaattttaaa atttgcacaa acgagagaac tcattaagaa acaagcagaa caatttttag
     1021 ccaacaattt ctgatggtgc tctgtaagcc gcactacaca aggctttgcg aaagtgatgt
     1081 aaaagtgaag aaagctaaca ttgcactttt aagctacttt ttcattgaat ttttagtatg
     1141 gaattacaca ggaataagtt agggatattc tatgatttac acagggaata tagggggatt
     1201 atggcacaca catagaaagg cattacacag agtgagtgct atgtgacaaa tggatgtaac
     1261 gagaggttac attcagctat ctgaaatgct ctctgtaaga aagttggtag taacgatacg
     1321 ttacatcgac agatgattgg atgtaacggt aggtcactgt gaactagggg ggtagttcac
     1381 tatgagctag ggggtaggtc atagtgagct agggggtagg tcactgtgag ctagggggta
     1441 ggtcactgtg aactacaata agactattaa c
//
LOCUS       DAZKIV010000348         1466 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZKIV010000348 DAZKIV010000000
VERSION     DAZKIV010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.351"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1466
                     /locus_tag="ACHOT6_004983"
     CDS             <1..>1466
                     /locus_tag="ACHOT6_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9950008.1"
                     /translation="RIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDE
                     YGRLTEKTDRIPTGVIRTDDERTHHYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGR
                     RTGKRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFTPLI
                     RVETENGEREKAQRRSLAEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSES
                     RVWLAQCGLTVEQLARQVEPEYTPARKVHFYHCDHRGLPLALISEDGNTVWSAEYDEW
                     GNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRNRYYDPLQGRYITQDPIGLAGGWSL
                     YAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAWDILSDTYEDMKRLNLGGTDQFF
                     HCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNLFGMYGRKVKLSHSEMIEDNKKD
                     LTVNDHGLTCPSTTDCSDRCSDYINPEHKKTIKALQDA"
BASE COUNT          392 a          348 c          456 g          270 t
ORIGIN      
        1 ccgcatcccg tatgccaccg acccggcggg aaaccgcctg ccggacccgg agctacaccc
       61 ggacagcacg ctcacggcgt ggccggataa ccgcatcgca gaggatgcgc actatgttta
      121 ccactatgat gaatacggca gactgacgga gaagacggac cgcatcccga cgggtgtgat
      181 acggacggac gacgagcgga cccaccacta ccactacgac agccagcacc gcctggtgtt
      241 ccacacgcgg atacagcatg gcgagccact ggtcgagagc cgctacctct atgacccgct
      301 gggccgccgg acgggaaaac gggtatggcg gcgggagcgt gacctgacgg ggtggatgtc
      361 gctgtcgcgt aaaccggagg tgacatggta cggctgggac ggcgacaggc tgacgacggt
      421 acagaccgac accacgcgta tccagacggt ataccagccg gggagcttca cgccgctcat
      481 ccgggtcgag acagagaacg gcgagcggga gaaagcgcag cgccgcagcc tggcggagaa
      541 gttacagcag gaagggagcg aagacggtca cggtgtggtg ttcccggctg aactggtgcg
      601 gctgctggat agactggagg aagaaatccg ggcagaccgc gtgagcagtg aaagccgggt
      661 gtggcttgcg cagtgcgggc tgacggtgga gcaactggcc agacaggtgg agccggaata
      721 cacaccggcg cgaaaagttc atttttacca ctgcgaccac cggggcctgc cgctggcgct
      781 catcagcgaa gacggcaata ctgtgtggag tgcagaatac gatgaatggg gcaaccagct
      841 taatgaggag aacccgcatc acctgcacca gccgtaccgg ctgccggggc agcagtatga
      901 taaggagtcg gggctgtact acaaccggaa ccggtactac gatccgttgc aggggcggta
      961 tatcactcag gacccgatag ggctggcggg gggatggagt ctgtatgcgt atccgctgaa
     1021 tccggtgaat ggtattgatc cattagggtt aagtcccgca gatgtagcgc taataagaag
     1081 aaaagatcaa ctaaaccatc aaagagcatg ggatatatta tctgatactt atgaagatat
     1141 gaagagatta aatttaggtg ggactgatca atttttccat tgtatggcat tttgtcgagt
     1201 gtctaaatta aatgacgctg gtgttagccg atcggcgaaa gggctgggtt atgaaaaaga
     1261 gattagagat tacgggttaa atctgttcgg tatgtacggc agaaaagtaa agctatccca
     1321 ttctgaaatg attgaagata ataaaaaaga cttgactgta aatgaccatg ggttgacatg
     1381 tccatcaaca acagattgct cagatagatg tagtgattat attaatccag agcataaaaa
     1441 aacgataaag gctttacaag atgctg
//
LOCUS       DAZKIV010000349         1457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZKIV010000349 DAZKIV010000000
VERSION     DAZKIV010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.352"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1360)
                     /gene="nleA"
                     /locus_tag="ACHOT6_004984"
     CDS             complement(<1..1360)
                     /gene="nleA"
                     /locus_tag="ACHOT6_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFP9950009.1"
                     /translation="MNIQPIVQSGITTQHNQHNQAEQAPPAQIPQSELPNGCEAGFVV
                     HIPDDIQQHAPECGERTALLSLIQDEGLLSGLDKYLAPHLEEGSAGKKALDMFGLFNV
                     TQMALEIRSTVPGISGKYGVQMNIVKPDIHPTSGNYFLQIFPLHDEIGFNFKDLPGPL
                     KNALSNSNISTTAVSTIASAATSAATSAASTAVSAITSAATSATTSTVTTEPKDLIPW
                     FGLTAQVVRNHGVELPIVKTENGWKLVGETPLTPDGPKANYTEEWVIRPGEVDFKYGT
                     SPLQATLGLEFGAHFKWDLDNSNTKYAVLTNAAANALGAAGGFAVSKIPGVDSMLAPH
                     IGAMVGQAAGHAIQCNTPGLKPDTILWWAGTTLGLADLNKAETGEARFTDYPRIWWHA
                     REGAIFPNKEDIARATGADIRAMEEGVPVDHRHPHPEDVVIDIESSPHNNPSNYVDTV
                     DII"
BASE COUNT          380 a          317 c          315 g          445 t
ORIGIN      
        1 ggattatatc aaccgtatca acataatttg atggattatt atgtggagag ctttcgatat
       61 cgatgaccac atcttcagga tgtggatgtc gatgatcaac agggacacct tcttccatcg
      121 cacgtatatc agcccctgtt gcacgggcaa tatcttcttt attggggaag atggctcctt
      181 ctcttgcatg ccaccatata cgaggatagt cagtgaatct ggcttctccg gtctcggctt
      241 tgtttaaatc tgctagcccc agagtcgtac cagcccacca taaaatagtg tctggcttta
      301 atccgggggt attacactgt atggcatgcc ctgctgcttg cccaaccatt gcgccgatat
      361 gaggggctaa cattgagtct actccgggga ttttggatac tgcaaaccct cctgcggcgc
      421 caagcgcatt tgcggcagca ttggtaagaa cggcatattt agtattagag ttatctaaat
      481 cccacttgaa atgtgcgcca aactccagcc ctagagttgc ctgtagtgga gatgtaccat
      541 atttaaaatc tacttctccc ggtctgataa cccactcctc cgtgtaattt gcttttggcc
      601 catcaggagt aagaggcgtt tctccaacaa gcttccatcc attttcagtt ttgactatag
      661 gaagttccac accatggtta cgaactactt gagctgttaa tccaaaccat ggtattaggt
      721 cttttggctc agtggttacc gtcgaagtag tggctgatgt tgcagccgat gtaatagccg
      781 acactgcagt ggatgcagcg gatgttgcag cagatgttgc agcagatgca atagtcgaca
      841 ctgcagtggt tgatatatta ctgttgctta atgcattttt taacgggcca ggaaggtctt
      901 taaaattaaa acctatttca tcatgcagag ggaatatctg taaaaaataa ttacctgatg
      961 taggatgaat atctggtttt acaatgttca tctgaacacc atatttacca gagataccag
     1021 gaacggtgct gcgtatttct aatgccatct gagtgacatt gaataaacca aacatatcca
     1081 acgctttttt ccctgcagaa ccttcttcaa gatgaggtgc aagatattta tccagcccag
     1141 agagcagacc ttcatcttgt atcaaactca atagtgccgt tctttcacca cattccggag
     1201 catgttgctg tatatcatct ggaatatgaa caacaaatcc cgcttcgcat ccattaggta
     1261 attcggattg cggtatttgt gcagggggcg cttgttctgc ctgattatgt tgattgtgtt
     1321 gtgtggtgat tccggattgt acgatcggtt gaatgttcat gataaatcct tatgacgtaa
     1381 tttatatcca ttaataatat caatccattg atattatcag tgtcacatat ccgatgtgga
     1441 cagctttaat atatagc
//
LOCUS       DAZKIV010000350         1452 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZKIV010000350 DAZKIV010000000
VERSION     DAZKIV010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.353"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            322..528
                     /locus_tag="ACHOT6_004985"
     CDS             322..528
                     /locus_tag="ACHOT6_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9950010.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     GLLTYLTNLYFKIREDRRKTARGD"
     gene            533..868
                     /locus_tag="ACHOT6_004986"
                     /pseudo
     CDS             533..868
                     /locus_tag="ACHOT6_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309990.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
BASE COUNT          408 a          283 c          397 g          364 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagcc agcgcccact ctgaaggacg
       61 ctggctaaaa caggtagatg tacttcacat gatacttata tttggcagta cattttctga
      121 cagacagtga cggatgttgt caagatattg tgtcatttat aacctgaatc aggggttggt
      181 cggaatgtta tctggcattt ttagcagagc ctgaatgcca taatcacggc tcccggagtt
      241 ggccgtcagt gggtgacact ggcggctttt tgttttcctt tactttcatt ttctgtcggc
      301 ggtgacggag acatacatca gatggaaaaa atcacaacgg gtgtgtcata caccacgtca
      361 gcggtgggga cgggatactg gttactgcag ctgctggaca aagtctctcc gtcccagtgg
      421 gtggcaatag gtgtactggg gagtctgctg tttggcctgc tgacgtatct gactaacctg
      481 tatttcaaaa tcagggagga ccgccgtaag acggcgcggg gagactaaag cgatgaagaa
      541 aaaatacgaa ctggttgtta aagagataaa taattacccg gataagattg ctgttactgt
      601 ggcacttgaa attggcgggc atccgtcgtt gttgttgcca catgtggcga ttagtcttga
      661 ccgtactgaa ggtgccacgc tggagttcta cgaagctgag gcgaaaaaac aggcgaagca
      721 gtttttcatg gatattgttg ccgggttatg cgaaggggat gaaccgtcac cggaaaagcg
      781 ccccgtaatt ttagatgcgc aggatgtatt gataacctac aaaggaaagc taccgggaag
      841 aattacttgt tctctgaaga tgccgccgtc aacactgcgg tcagaaaaag atgatgttga
      901 atcgcgtatt gaaaaactgg agtgctatat cgctgaattg aaaaaaagca ccccaacaaa
      961 aaatgaggtg cttgcagcag acgaaatgaa agaagctatt cctgatcgcg cggcgaatct
     1021 aagctgcgct tcatggttga aagagcatct tcagcagcct gaaaaaaaac gccgcgatga
     1081 gcagtttgct gcgttttacg attattgccg gaaagtgatg agcagaaatc tcgcagagtg
     1141 tttcagtatt cataatgata atttcagtga cctggaatgg gagtgtaacc ggccatcctt
     1201 tgttgtatcc ggtgatgctg ggaaaataac catctcagaa aatggaaaag taacaccgcc
     1261 atcgcaccag catagtgagg agctcattga atttgccatt gattacctga agaacaataa
     1321 aaagcagggg ctgatgaagt gcattggtcg ttgcatggga tatctgcaga tagctgctga
     1381 gattgaagcg ctggccagtg gtgcggacaa ggatgcagtt gtgcgggagg ctcttcttcg
     1441 tgattttgat aa
//
LOCUS       DAZKIV010000351         1447 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZKIV010000351 DAZKIV010000000
VERSION     DAZKIV010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1447
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.354"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..468
                     /locus_tag="ACHOT6_004987"
     CDS             <1..468
                     /locus_tag="ACHOT6_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFP9950011.1"
                     /translation="ARIKGSQLAKDPEVLAFIARKQCETVEVDEVPVYRQKKSEQEDK
                     PRRREVAAIPQPDEKNPEMPPPAVISHGIEYMEDGLPDPVKAMGQILVENLIIDPKLA
                     LDAAWRLAQFTHHKKGDAGKKSAKGDAAKKAANRFSVPPSPRLVVNNQNEESG"
     gene            465..>1447
                     /locus_tag="ACHOT6_004988"
     CDS             465..>1447
                     /locus_tag="ACHOT6_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFP9950012.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPAPIYPDQAAHALAIFKQLRI
                     VDAPGSPTFGESCAQWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQYKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEEAFRGFMSKHANVEIGLALRSDRWAG"
BASE COUNT          344 a          363 c          441 g          299 t
ORIGIN      
        1 gccaggatta aaggctcgca gctggcaaaa gatccggagg tgctcgcgtt tatagcccgt
       61 aaacagtgcg aaacggtgga ggtggatgag gttcctgttt accggcagaa aaaatcagag
      121 caggaggata aaccccgtcg ccgtgaggtg gctgcaatac cacagccgga cgaaaaaaat
      181 ccggagatgc ctccgcccgc ggtgatatct catggtattg aatatatgga ggatggtctt
      241 cccgatccgg tgaaagctat ggggcagatc ctggtggaaa acctgataat tgacccgaaa
      301 ctggcactgg atgcggcctg gcgactggcg cagttcacac accataaaaa aggcgatgcc
      361 ggtaaaaaat cggcaaaagg tgatgccgcg aaaaaagcgg ctaaccgttt ttcggtgcca
      421 ccatctcccc gactggtggt gaataaccag aatgaggaaa gcggatgata cctgtatgga
      481 gcacggcatg tccggactgg gcagagcgcc tgaaaaaggg actgtcgatt attccggctc
      541 cgatttatcc ggaccaggct gcacatgcac tggcgatttt taaacaactg cggattgtgg
      601 atgcaccggg tagcccgacg ttcggggagt cctgcgcaca gtgggtgttt gacctggtgg
      661 cggccctgtt tggctcctat gatgcgcaga ccggtgtacg ccatatcaag gaagttttta
      721 tccttatccc caagaaaaac agtaagtcca cgctggccgc ggggatcatg atgacggcgc
      781 tgttactgaa ctggcggcag gcggcgggct acaccattct ggccccgacc gtggaggtgg
      841 cggctaacgc cttcaaccct gccagggata tggtacgacg ggacgatgat ctggatgacc
      901 tctgtcaggt gcagacacat atccggacca tcacccatcg ggtgacggac accaccctga
      961 aggtggtggc agccgatccg aatacggtgt ccggtatcaa gtccgtgggg acactgattg
     1021 atgaactgtg gctgtttggc aagcagtaca aagcggaaga catgctacgt gaagccatcg
     1081 gcgggcttgc ctcccgtccg gaaggatttg tggtgtacac aaccacccag tcgaatgaac
     1141 cgcctgccgg ggtgttcaga cagaaactgc agtacgcccg ggatgtccgt gacggcaaaa
     1201 ttcatgatcc gcactttctg ccggtgattt ttgagcatcc tcctgaaatg gtggaaagcg
     1261 gggctcacct gctgatggaa aacctcgcca tggttaaccc gaatctcggt tattcggtgg
     1321 atgaggcttt tctgtaccgg gagtaccgta aagcccggga ggctggtgag gaagcatttc
     1381 gtggcttcat gtcaaaacac gccaatgtgg aaatcggtct tgccctgcgt tctgaccgct
     1441 gggcggg
//
LOCUS       DAZKIV010000352         1411 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZKIV010000352 DAZKIV010000000
VERSION     DAZKIV010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.355"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..483
                     /locus_tag="ACHOT6_004989"
     CDS             <1..483
                     /locus_tag="ACHOT6_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFP9950013.1"
                     /translation="FRENGITTMEQVNAGMRVARRQNRPFLPSPGQFVAWCREEASVI
                     AGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNLYQNMRANALTDAELR
                     RKAADELVHMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAKIAEIKAKFGLKGASV
                     "
     gene            480..770
                     /locus_tag="ACHOT6_004990"
     CDS             480..770
                     /locus_tag="ACHOT6_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFP9950014.1"
                     /translation="MTGKEAIIHYLRTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLVFKECRQSAAMKRVLAVYGVKR"
     gene            844..1284
                     /locus_tag="ACHOT6_004991"
     CDS             844..1284
                     /locus_tag="ACHOT6_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFP9950015.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTS
                     RMRVGEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            1281..>1411
                     /locus_tag="ACHOT6_004992"
     CDS             1281..>1411
                     /locus_tag="ACHOT6_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA N-6-adenine-methyltransferase"
                     /protein_id="HFP9950016.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNAL"
BASE COUNT          383 a          315 c          406 g          307 t
ORIGIN      
        1 ttccgggaaa acgggatcac cacaatggaa caggttaacg caggaatgcg cgtagcccgt
       61 cggcagaatc gaccatttct gccatcaccc gggcagtttg ttgcatggtg ccgggaagaa
      121 gcatccgtta tcgccggact gccaaacgtc agcgagctgg ttgatatggt ttacgagtat
      181 tgccggaagc gaggcctgta tccggatgcg gagtcttatc cgtggaaatc aaacgcgcac
      241 tactggctgg ttaccaacct gtatcagaac atgcgggcca atgcgcttac tgatgcggaa
      301 ttacgccgta aggccgcaga tgagcttgtc catatgactg cgagaattaa ccgtggtgag
      361 gcgatccctg aaccagtaaa acaacttcct gtcatgggcg gtagacctct aaatcgtgca
      421 caggctctgg cgaagatcgc agaaatcaaa gctaagttcg gactgaaagg agcaagtgta
      481 tgacgggcaa agaggcaatt attcattacc tgaggacgca taatagcttc tgtgcgccgg
      541 acgttgccgc gctaacaggc gcaacagtaa ccagcataaa tcaggccgcg gctaaaatgg
      601 cacgggcagg tcttctggtt atcgaaggta aggtctggcg aacggtgtat taccggtttg
      661 ctaccaggga agaacgggaa ggaaagatga gcacgaacct ggtttttaag gagtgtcgcc
      721 agagtgccgc gatgaaacgg gtattggcgg tatatggagt taaaagatga ccatctacat
      781 tactgagcta ataacaggcc tgctggtaat cgcaggcctt tttatttggg ggagagggaa
      841 gtcatgaaaa aactaacctt tgaaattcga tctccagcac atcagcaaaa cgctattcac
      901 gcagtacagc aaatccttcc agacccaacc aaaccaatcg tagtaaccat tcaggaacgc
      961 aaccgcagct tagaccaaaa caggaagcta tgggcctgct taggtgacgt ctctcgtcag
     1021 gttgaatggc atggtcgctg gctggatgca gaaagctgga agtgtgtgtt taccgcagca
     1081 ttaaagcagc aggatgttgt tcctaacctt gccgggaatg gctttgtggt aataggccag
     1141 tcaaccagca ggatgcgtgt aggcgaattt gcggagctat tagagcttat acaggcattc
     1201 ggtacagagc gtggcgttaa gtggtcagac gaagcgagac tggctctgga gtggaaagcg
     1261 agatggggag atcgggctgc atgactatca aatcaaatac gccagcacac gacaaggact
     1321 gctggcaaac gccgctttgg ctttttgatg cactggatat tgagtttgga ttctggctgg
     1381 attcggcagc gagcgacaaa aatgctctgt g
//
LOCUS       DAZKIV010000353         1407 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZKIV010000353 DAZKIV010000000
VERSION     DAZKIV010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1407
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.356"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1407
                     /locus_tag="ACHOT6_004993"
     rRNA            <1..>1407
                     /locus_tag="ACHOT6_004993"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          355 a          324 c          446 g          282 t
ORIGIN      
        1 gagtaatgtc tgggaaactg cctgatggag ggggataact actggaaacg gtagctaata
       61 ccgcataacg tcgcaagacc aaagaggggg accttcgggc ctcttgccat cggatgtgcc
      121 cagatgggat tagctagtag gtggggtaac ggctcaccta ggcgacgatc cctagctggt
      181 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
      241 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      301 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      361 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      421 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      481 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      541 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      601 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      661 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      721 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      781 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      841 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacgga agttttcaga
      901 gatgagaatg tgccttcggg aaccgtgaga caggtgctgc atggctgtcg tcagctcgtg
      961 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
     1021 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
     1081 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
     1141 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1201 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1261 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1321 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1381 tcgtaacaag gtaaccgtag gggaacc
//
LOCUS       DAZKIV010000354         1400 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZKIV010000354 DAZKIV010000000
VERSION     DAZKIV010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.357"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1400)
                     /locus_tag="ACHOT6_004994"
     CDS             complement(<1..>1400)
                     /locus_tag="ACHOT6_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFP9950017.1"
                     /translation="WGLSLPSLRRRLFRCVSIRENTDGTFAITAVQHVPEKEAIVDNG
                     AHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTA
                     DDGSERLVSTARTTETTYRFTQLALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAAPS
                     RIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIADIRQVETTARYLGTALYWIAAS
                     INIKPGHDYYFYVRSVNTVGKSTFVEAVGRASDDAEGYLDFFKGLITESHLGKELLEK
                     VDLTEDNASKLEQFSKEWKDANDKWNAMWGVKIEQTKDGKYYVAGIGLSMEDTEEGKL
                     SQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDVFLKRLTAPTITSGGNPPSFSLT
                     PDGKLTAKNADISGSMNANSGTLNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRT
                     NSYASGTITVRISDDQ"
BASE COUNT          275 a          415 c          360 g          350 t
ORIGIN      
        1 ttctgatcat cactgattct taccgtgatg gtgccactgg cataactgtt cgtgcggggg
       61 aaagacttgc tgaccgtttt gacaatatcg ccttcaatct gattggctga cagtttcccc
      121 ttaatctgac agttctcatt aatcgtgacg ttgttgagcg tcccggagtt cgcattcata
      181 ctgccactga tatccgcatt tttagcagtc agctttccgt ccggtgtcag ggaaaatgac
      241 ggtggatttc caccgctggt aatggtgggg gccgtcaggc gcttcaggaa cacgtcgttc
      301 atgaatatct ggttgccctg cgccacaaac atcggcgttt cattcccgtt tgccgggtca
      361 ataaacgcga tacggttagc ggcaaccaga aactggctca gtttgccttc ctccgtgtcc
      421 tccatgctga ggccaatacc cgcgacataa tatttgccgt ctttggtctg ctcaattttg
      481 acgccccaca tggcattcca cttatcgtta gcatccttcc actctttcga aaactgctcc
      541 agtttgctgg cgttatcctc cgtcaggtcg actttttcca gcagctcttt accgagatgg
      601 gattcggtta tcaggccttt gaaaaaatcc aggtaacctt cggcatcatc gctcgcccga
      661 ccgacggcct ccacaaatgt cgatttgccg actgtgttca cactgcgaac gtaaaaataa
      721 taatcatggc ccggcttaat attgatactg gcggctatcc agtacagcgc cgtgccaaga
      781 tagcgggctg tggtttcaac ctgcctgata tccgcaatcc gtttttccga gaaccagaac
      841 tcaaactgta ccgtcgggtc ataaaccgca agatgtggcg tggcggttat ctgaaaatag
      901 cccggtgtca gctcaatccg agacggcgct gccggtgcgg caatccggaa cgataccgat
      961 gccggatcgc cctgctgtcc ccacgcattt accgcccgga ctgtcagtct gtagttcccc
     1021 agcgccagtt gcgtgaagcg gtatgtggtt tccgtcgtcc gggccgtgct gaccagccgc
     1081 tcactgccgt catccgctgt tacggtcaga cggagcagga agctcacgcc cttcaccacc
     1141 ttcggcgtgt cccagcgcgc cagcacctga tattccccgc tgtctgcggt gacttcggcg
     1201 gtcaggtgct gcaccgctgg cggcgtgaca ccattcaccg tgccgctctg gtcgccgtca
     1261 aagtgcgccc cgttatccac gatggcttct ttttccggta cgtgctgcac cgccgtgatg
     1321 gcaaaggtgc cgtccgtgtt ttcccggatg gaaacacagc ggaacaggcg acgacgcagt
     1381 gacggcaggg agagtcccca
//
LOCUS       DAZKIV010000355         1386 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZKIV010000355 DAZKIV010000000
VERSION     DAZKIV010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.358"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..738)
                     /gene="tssI"
                     /locus_tag="ACHOT6_004995"
     CDS             complement(<1..738)
                     /gene="tssI"
                     /locus_tag="ACHOT6_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFP9950018.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQ"
BASE COUNT          386 a          339 c          326 g          335 t
ORIGIN      
        1 ctggccttcc tgatcaaaac gccctgccca gccgggtcgt ttaaaggtgt agtccttggt
       61 caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc agagggtgct
      121 cacctcggta cgggtgttcg gattccaggg gatctcaaag gactccggca gataacgcac
      181 ggtgtcgcac aggaccaggc tctggtcggt acttttttgc gcgtgctcct cataaaagaa
      241 gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtttcgc cgtactggac
      301 acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccactcgg tcacaccgtt
      361 ttctttcagg atcgtagcga ggatgctttc gatgtcctca ttctggaaga tacggaagtt
      421 ctggcgcagc cctgtgcgcc acaacggtgg gcacaccttc atgctgtata gcatctggtt
      481 tttgtcgttc tcccccagtt caaaccaggt caccacacct ttcacccggc gctgtacgtc
      541 atcgccctgc cataccgtca ggtaggccat tttgtccagc acctgcgcaa attcaaggga
      601 gagaaactgc tggctgacca gagagagatc gagggaaaag agcgaagaga gtgactggtt
      661 caaatgaaag gaaaccaccg caaaagcatc cggtggcagg ccgtccactt ccagtgtgaa
      721 acgtaatccg gttgacatag caacctccgt gaaaatgaat aataatccgc acatattgca
      781 tgtacaacat gtcgggcgga tgagataagc gagtcgacat ctggcgttac cggggaatat
      841 cagaaaaatg tcatggttat gtgatgattt agtctgggat tttacttcag agaaacgggt
      901 gtctgtttag tcaacaaaat aatcacaaac gcaacggatg ggttctcgac gttattcacg
      961 acaggctcgt acattacagc gagtaataaa aaacctgata tgcaaagtca aaccaatatt
     1021 cataatgtct ttttattcac aacaataaat aattcaataa caatcattga aatgaatgat
     1081 gatttgtata aggaatatac gctaccgccc caccctgata acatgatctg cacattgcca
     1141 tcttaacgtc gttaaaaact ggcttaatag tggatatttc aaattaggaa tttattaaat
     1201 tcacctgatg aatatcataa gcaaatagca tagaaaataa tgacaattcc cccgagggga
     1261 ccatttccaa agcactgcac caccacctgg tgcccggatg atcttgcttc agtggtaaga
     1321 gtacctgatt cattgttccc aaaatgatgt tttccccggc cccgccaata atgtgcagct
     1381 ctcacg
//
LOCUS       DAZKIV010000356         1368 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZKIV010000356 DAZKIV010000000
VERSION     DAZKIV010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.359"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            356..1345
                     /locus_tag="ACHOT6_004996"
                     /pseudo
     CDS             356..1345
                     /locus_tag="ACHOT6_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          397 a          323 c          333 g          315 t
ORIGIN      
        1 gggggctaaa attaattcta tttcattata tgagaacacc aataagaata ataatatttg
       61 atcacaactg aagatgcttt ttacaatgaa gaattattgt taaaaaacgc tttgttgaat
      121 caaccgaact gttgctgagt tgcaggatca catcacgtat tttcctgaaa acgcaggctg
      181 cttagtagca aagcagaagt tcaaaatcac caactaaata gctgcgccga atagtagatc
      241 aatgaaaggg aactcagccc ggattgtgcg atctgatcaa tcgccaaaaa tcccccccta
      301 accggactga gcgatgccga tcatagcacc aattccccgc gacgaatgac gcctgatgca
      361 aaaagcaatc tataaaactc acgataaaaa ttatgcccgc aggctgaccg ccatgctgat
      421 gctgcaccgg agcgctcgcg tcagtgacgt tgccagaacg ctctgtttcg cccgttcatc
      481 tgtcgggcgc tggattaact ggttcacaca gtcgggtgtt gatggactga aatcattacc
      541 cgccgggcgt gcccgccgct ggtcatttga acatatctgt acactgttac gtgagctggt
      601 aaaacattct cctggcgact ttggctacca gcgttcacgc tgaagcacag aactgttggc
      661 aataaaaatc aatgagataa ccggatgtca gttacatgcc ggaaccgtgc gccgctggtt
      721 gtcgtctgcg gggctggtgt ggcgcagagc agcgccaact ctgcgtatcc gtgacccgca
      781 taaagatgaa aagatggtgg caattcataa agcactggac gaatgcagcc cagagcatcc
      841 ggtattttat gaagatgaag tggatatcca ccctaatccc aaaatcggtg cgggctggca
      901 gcttcgcgga cagcaaaaac acgtggtgac gccgggtcag aatgaaaaat attatctggc
      961 tggcgcgtta cacagcggaa cgggaaaagt gagttatgtg ggtggcaaca gcaagagctc
     1021 cactttattt atcagtctgt tgaaacacct gaaatcgaca taccgacggt ctaaaactat
     1081 catgctcatc gtggacaact acatcatcca caaaagccgg gaaacacagc gctggctgaa
     1141 gcagaatccg aagttcaggg tgatttatca gctggtttac tctccatggg tgaatcacgt
     1201 tgaacggcta tggcaggcgc ttcacgacac aataacgcac aaccatcagt gccgttcaat
     1261 gtggtagttg ctgaaaaaga ttcaccattt tatgggaacc gtcagcccat tccccggagg
     1321 taattatgga caggcaaaag tgtagcggta ttaggcacag ctatttag
//
LOCUS       DAZKIV010000357         1314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZKIV010000357 DAZKIV010000000
VERSION     DAZKIV010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.360"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..557)
                     /locus_tag="ACHOT6_004997"
     CDS             complement(<1..557)
                     /locus_tag="ACHOT6_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFP9950019.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMRVFHGGRVLVESEPKSMRN
                     LPSGVVPAVRQPLAEDKSLLPFFSNERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
     gene            complement(559..837)
                     /locus_tag="ACHOT6_004998"
     CDS             complement(559..837)
                     /locus_tag="ACHOT6_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950020.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHOT6_004999"
     CDS             complement(1005..1160)
                     /gene="hokD"
                     /locus_tag="ACHOT6_004999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFP9950021.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRVRTGQTEVAVF
                     TAYEPEE"
BASE COUNT          298 a          349 c          359 g          308 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcatggatt cacggcctgg cttaacgata accagaccga gttccggtac cagaacaggt
      541 cgaagtaata cccgcacatt acctccagat ccgttgctgg aatgtgcggg acggacgcgg
      601 tggccgttcg gagtaaggga gcctgacgga gattatccag tgacggtagt cgaggctaag
      661 ggctttttta acctcgcatc cgcgcctgcg gtaacactga atgagccatt cggcctgttc
      721 ttcagtgcat gggggatgct ggtaccagtc agatttgaat gcgtgaaaac accgtccgcg
      781 cctgctggca aagacggcag aatcatcaga attgtataat ttggtatcgt gcgccatcgg
      841 ttgtctctgc tggcgcagca ggtgccagtt gttcaggctg gcgtgcgaat tgtaaaccag
      901 aatgccagga aaaaacaaaa cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc
      961 tgattcatca gaggtggcga gggatttctc ccccgccggg tctcttactc ctcaggttcg
     1021 taagctgtga agacagcgac ctccgtctgg ccggttcgga ctcgtacctc gcagaggtct
     1081 ttcctcgtta ccagtgccgt cactatgacg gttaaacaga tgacgatcag ggcgattaac
     1141 atcgcctttt gctgcttcat agcctgcttc tccttgcctt tcggcacgta agaggctaac
     1201 ctacgtttgt gaagcataga ttgggcctca gattaatgtt aagcgtcttg caggacgcgt
     1261 aatgttaact ggggcttttc tctgtctgcc ttacggtggc atgcccgagg caga
//
LOCUS       DAZKIV010000358         1306 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZKIV010000358 DAZKIV010000000
VERSION     DAZKIV010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1306
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.361"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            30..236
                     /locus_tag="ACHOT6_005000"
     CDS             30..236
                     /locus_tag="ACHOT6_005000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFP9950022.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTTTSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            233..>1306
                     /locus_tag="ACHOT6_005001"
     CDS             233..>1306
                     /locus_tag="ACHOT6_005001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9950023.1"
                     /translation="MKMSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTR
                     PSRLFRTQFRMVSPKRISNPNNTSDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYSAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSL"
BASE COUNT          295 a          343 c          409 g          259 t
ORIGIN      
        1 tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca ggaagaactt gccgctgccc
       61 gtgcggcact gcatgacctg atgacaggaa aacgggtggc aacggtacag aaagacggac
      121 ggcgagtgga gtttacgacc acttccgtgt ctgacctgaa aaaatacatt gctgagctgg
      181 aagtgcagac cggcatgaca cagcgacgca ggggaccagc aggattttat gtatgaaaat
      241 gtccaccatt cccacccttc tggggccgga cggcatgaca tcgctgcgtg aatatgccgg
      301 ttatcacggc ggtggcagcg gatttggtgg gcagttgcgg gcgtggaacc caccgggtga
      361 aagtgtggat gcagccctgc tgcccaactt tacccgtggc aatgcccgcg cagacgatct
      421 ggtacgcaat aacggctatg ccgccaacgc catccagttg catcaggatc atatcgtcgg
      481 gtcttttttc cgactcagtc atcgcccaag ctggcgctat ctgggcatcg gggaggaaga
      541 agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa gagtttgccg aagatgactg
      601 ttgctgcatt gacgttgagc gaaaacgcac gtttaccatg atgattcggg aaggtgtggc
      661 catgcacgcc tttaacggtg aactgttcgt tcaggccacc tgggataccc gtccctcgcg
      721 actgttccgg acacagttcc ggatggtcag cccgaagcgc atcagcaacc cgaacaatac
      781 cagcgacagc cggaactgcc gtgccggtgt gcagattaat gacagcggtg cggcgctggg
      841 atattacgtc agcgaggacg ggtatcctgg ctggatgccg cagaaatgga catggatacc
      901 ccgtgagtta cccggcggtc gtgcttcgtt cattcacgtc tttgaacccg tggaggacgg
      961 gcagacccgc ggtgcaaatg tgttttacag cgtgatggag cagatgaaga tgctcgacac
     1021 gctgcagaac acgcagctgc agagcgccat tgtgaaggcg atgtatgccg ccaccattga
     1081 gagtgagctg gatacgcagt cagcgatgga ttttattctg ggcgcgaaca gtcaggagca
     1141 gcgggaaagg ctgaccggct ggattggtga aattgccgcg tattactccg cagcaccggt
     1201 ccgtctggga ggcgcaaaag tgccgcacct gatgccgggg gactcactga acctgcagac
     1261 ggctcaggac acggataacg gctactccgt gtttgagcag tcactg
//
LOCUS       DAZKIV010000359         1304 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZKIV010000359 DAZKIV010000000
VERSION     DAZKIV010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.362"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..192)
                     /locus_tag="ACHOT6_005002"
     CDS             complement(<1..192)
                     /locus_tag="ACHOT6_005002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950024.1"
                     /translation="MDYTNLPKQTFADLIALRQAVVALINLLPEKEKELVKALLNRTA
                     ADFSSYPLTDDLADLPELIA"
     gene            306..839
                     /locus_tag="ACHOT6_005003"
     CDS             306..839
                     /locus_tag="ACHOT6_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9950025.1"
                     /translation="MNVKIRYSLSAAVLALIAASAPAPDILDQFLDEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCAQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VIRRDQESALTCWGIEQ"
     gene            836..>1304
                     /locus_tag="ACHOT6_005004"
     CDS             836..>1304
                     /locus_tag="ACHOT6_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2514 domain-containing protein"
                     /protein_id="HFP9950026.1"
                     /translation="MNQIFTVILLVLVGFVVGNVWSDRGWQKKWAERDAAELSQEVNV
                     QFAARIIEQGRTISRDEAVKDAQQKAAEISARAADLSDSVNQLRAEATKYAIRLDAAQ
                     HTANLAAAVRGKTTKAAEGMLTNMLGDIAAEAQLYAEIADKRYIAGVTCQRIYE"
BASE COUNT          352 a          285 c          342 g          325 t
ORIGIN      
        1 tgcaattaat tcaggaaggt ccgcaaggtc atctgtcagt ggatatgatg aaaaatcggc
       61 ggcagttctg ttaagaagcg ctttaactaa ttccttttcc ttctccggca acaagttgat
      121 tagagctacg actgcttgcc tgagtgcgat taaatcagca aaagtttgtt ttggtagatt
      181 tgtgtaatcc atagtcacct ctgtgtttat cagattgaca tcctcctccc gccagtgccc
      241 atcactggtg aggtaagatt taacatatcc ggggatttga agccgataaa tcctgataaa
      301 tatccatgaa cgtaaaaatc agatacagcc tgtcggctgc tgttctggca ctgattgccg
      361 ccagtgctcc tgcccctgac attctcgatc agtttctgga tgaaaaagag gggaatcaca
      421 ccactgcata ccgcgatggc tccggcatat ggaccatctg tcggggagca acgatggtgg
      481 acggtaaacc tgttattccg ggaatgaaac tgtcgaagga aaaatgcgcc caggttaacg
      541 ccattgagcg ggataaggcg ctggcatggg tggagcgcaa tataaaagtt ccactgaccg
      601 agccacagaa agccggtatc gcgtcattct gtccctataa cattggccct ggtaagtgtt
      661 ttccgtcgac gttttataag cggcttaatg ccggtgatcg taaaggcgca tgcgaggcga
      721 ttcgctggtg gataaaggac ggtggacgtg attgccgcat tcgttcaaat aattgttacg
      781 gtcaggttat tcgtcgtgac caggagagtg cattaacctg ctgggggata gaacagtgaa
      841 tcagatattc acggtgattt tgctcgtgtt ggtaggattt gtcgtaggta atgtctggag
      901 cgacagagga tggcaaaaaa aatgggcgga gcgtgatgct gccgaattat ctcaagaggt
      961 aaatgtccaa tttgctgctc gaataattga acaggggcga actatatccc gtgatgaggc
     1021 tgttaaagat gcacaacaga aagccgctga aatttctgcc agggctgctg atctgtctga
     1081 tagtgttaac cagctgcgtg ccgaagcaac aaaatatgcc atacgccttg acgcagcgca
     1141 gcataccgca aatcttgccg ctgccgtcag aggcaaaaca accaaagccg ccgaaggaat
     1201 gctcaccaac atgctcggag atattgcagc agaagctcag ctttatgctg aaattgctga
     1261 caaacgctac atcgcaggag tgacttgtca acggatttat gaat
//
LOCUS       DAZKIV010000360         1285 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZKIV010000360 DAZKIV010000000
VERSION     DAZKIV010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.363"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..1205
                     /gene="espG"
                     /locus_tag="ACHOT6_005005"
     CDS             60..1205
                     /gene="espG"
                     /locus_tag="ACHOT6_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE effector EspG"
                     /protein_id="HFP9950027.1"
                     /translation="MINGLNNDSASLVLDAAMKVNSGFKKSWDEMSCAEKLLKVLSLG
                     LWNPTYSRSERQSFQELLTVLEPVSPLPNELGRVSARFSDGSSLRISVTNSESIEAEI
                     RTPDNEKIFVLLESNEQNRLLQSLPIDRHMPYIQVHRALSEIDLTDTTSMRNLLGFTS
                     KLSTTLIPHNAQTDPLSGPTPFSSIFMDTCRGLGNAKLSLNGVDIPANAQMLLRDALG
                     LKDTHSSPSRNVIDHGISRHDAEQIARESSGSDNQKAEVVEFLCHPEAATAICSAFYQ
                     SFNVPALTLTHERISKASEYNAERSLDTPNACINISISQSSDGNIYVTSHTGVLIMAP
                     EDRPNEMGMLTNRTSYEVPQGVKCTIDEMVRALQPRYAASETYLQNT"
BASE COUNT          416 a          274 c          241 g          354 t
ORIGIN      
        1 gatgctatat gatactagtt gccaaattgt tcattacaaa ccagatagga gaatctctca
       61 tgataaatgg acttaataat gactccgcat ctttagtttt agatgctgca atgaaagtta
      121 attctgggtt taaaaaaagc tgggatgaga tgtcatgcgc tgaaaagtta cttaaagtac
      181 ttagtcttgg tttatggaat ccaacgtaca gtcgtagtga aagacaatca tttcaagagt
      241 tgttaaccgt tttagagcct gtatctccac ttcccaatga attaggcaga gtatctgctc
      301 gtttttcaga tggctcatcc ttaagaattt ccgtcactaa cagcgaaagt attgaagccg
      361 agattcgcac accagataat gaaaagattt ttgtgctcct agagtcaaac gaacaaaata
      421 ggttattaca atctttaccc atcgatcgcc acatgccata cattcaggtt catcgtgcct
      481 tatctgagat tgacctgact gatactacct caatgcgcaa tctacttggt tttacgtcaa
      541 aactatcaac aaccttgatt ccgcataatg ctcaaacaga tccgctttcc gggcctacac
      601 cattcagctc tatctttatg gatacatgtc gaggactcgg caatgcaaag ctttcactca
      661 atggtgttga tatacctgca aatgcacaaa tgttgcttcg cgatgcacta ggacttaaag
      721 acacacactc atcaccttcc cggaatgtta tagatcatgg tatttctcgc catgatgcag
      781 agcaaatagc aagagaaagc agcggcagcg ataatcagaa agctgaagtt gtggaatttt
      841 tatgccatcc agaagcagca acggccatat gctcggcttt ctatcaatct ttcaatgtgc
      901 cagccttaac gttgacacat gaaaggatct ctaaagccag tgaatacaat gcggaaagat
      961 cgttagatac acctaacgct tgcattaaca taagtatctc tcaatcatca gatggaaaca
     1021 tttatgttac cagccatacc ggggttctga taatggcgcc agaagaccgc cccaacgaga
     1081 tgggcatgtt gacgaacagg acttcttatg aagtgcctca aggtgtgaaa tgtacaatcg
     1141 atgaaatggt aagagcgcta caaccaaggt atgctgcatc tgaaacgtac ttacaaaaca
     1201 cttaaaataa aactttatta atcaaaacca ataatagaaa ttacacccgc aattattaac
     1261 aatttaccct gaagttaatt acaat
//
LOCUS       DAZKIV010000361         1265 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZKIV010000361 DAZKIV010000000
VERSION     DAZKIV010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.364"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1265
                     /locus_tag="ACHOT6_005006"
     CDS             <1..>1265
                     /locus_tag="ACHOT6_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HFP9950028.1"
                     /translation="AMLTLQFHDILPGSCISRVYHETEKEYLKLEAKTEKIISDAQST
                     LLSKIDTSSYKDPHILFNTTCFARNEWININNNWLKARVNSYGYAVIDPKNKIVNGLK
                     AESRSIENNYIKLLFSESGDLISLYDKRYGKEYITENMHSEIRAYHEDAGFFAAWDFA
                     SNYRDGESYVLLAEKMTTVISGPKTTMTLIYHYNSSYLRFAFTLTQDSPRVDVQTFID
                     WHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDRPTHSDDSFAFAKDEIPAHHWVDLSDQ
                     ETGIALIAKNKYGYRVKDQTLELTLLRSQHKPGEVVKADNYDNFLINNFADIGKQKFT
                     FSLFPHHGDYRVGGVVNQAYIMNHPLQIVPVKPHPGELPPSLSFCAIDTNSVIIETIK
                     LAEDGDGIIVRLYESYGLEISAMLSWVCN"
BASE COUNT          404 a          250 c          233 g          378 t
ORIGIN      
        1 aagcgatgct gaccttgcaa ttccatgaca tccttcccgg ctcatgtatt tcaagagttt
       61 atcatgaaac tgaaaaggaa tatcttaaac tagaagcaaa aaccgagaaa attatttctg
      121 atgcgcagtc tactttatta agtaaaattg acacttcatc ttataaagat ccgcacattt
      181 tatttaatac cacctgtttt gctcgtaacg aatggatcaa tataaacaac aactggctca
      241 aagcacgagt aaactcttat ggttatgctg tgattgatcc aaagaataag atcgtaaatg
      301 gtctgaaggc agaatctcga agtattgaaa ataattacat taaacttctt ttttcagaaa
      361 gcggtgattt aatttcgcta tatgataaaa gatatggtaa agaatacatt actgaaaata
      421 tgcacagtga gatccgtgca taccacgaag atgcaggttt tttcgcggcc tgggattttg
      481 cttcaaatta tcgtgatggg gaatcatatg ttttactcgc tgaaaaaatg acaactgtga
      541 tcagcgggcc taaaactacg atgacgctga tttatcacta caattcctct tatcttcgct
      601 tcgcatttac cctgacacag gacagtccac gcgttgatgt ccaaactttt attgattggc
      661 acgaacctaa tgtttctctc aaagtaaaat tcccggtatc agtacaaaca tctcttgcac
      721 aatgtcaaat tcagttcggt gtaattgaca gaccaacgca tagcgatgat tcgttcgctt
      781 ttgccaaaga tgaaatacca gcacatcact gggtcgacct ttctgatcaa gaaactggca
      841 ttgctttaat agcaaaaaac aaatatggct atcgtgtcaa agaccagacg ctagagttaa
      901 ctttgttgcg ttcgcagcac aaacctgggg aagttgttaa ggcagataat tatgataatt
      961 tcctgattaa taattttgca gatattggta aacaaaaatt cacattctcc ttatttcctc
     1021 atcatggaga ttaccgtgtg ggtggtgtcg ttaaccaggc ctatataatg aatcatccct
     1081 tacagatcgt ccctgtcaaa ccacacccgg gtgaactacc cccttcacta tcattttgtg
     1141 ctatcgatac taacagcgtt atcatcgaaa caataaaatt agctgaagac ggcgacggta
     1201 taatcgtaag attatatgag agttatggat tagaaatttc agccatgctt tcgtgggtat
     1261 gtaat
//
LOCUS       DAZKIV010000362         1215 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZKIV010000362 DAZKIV010000000
VERSION     DAZKIV010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.365"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            132..734
                     /gene="ais"
                     /locus_tag="ACHOT6_005007"
     CDS             132..734
                     /gene="ais"
                     /locus_tag="ACHOT6_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFP9950029.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(773..1198)
                     /gene="nudI"
                     /locus_tag="ACHOT6_005008"
     CDS             complement(773..1198)
                     /gene="nudI"
                     /locus_tag="ACHOT6_005008"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFP9950030.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          326 a          276 c          259 g          354 t
ORIGIN      
        1 tggcaaaatt ttgaggcatt ttgccgacat cgtaggattt ttaatattac accaactgct
       61 aattatcgcc agaaatgtcg ctcaatttta cggtaattgt ctggttgcgc ttgtctatag
      121 gtggagttta cgtgttagct ttttgccgct catcattgaa gtcaaaaaaa tatttcatca
      181 ttttactggc gctcgctgca attgccggac tgggtactca tgccgcctgg agtagcaatg
      241 gtttgccacg tatcgacaat aaaacattgg ccagactggc acagcagcac ccggttgtcg
      301 ttttgtttcg ccatgctgaa cgttgcgatc gttcaaccaa tcaatgcttg tcagataaaa
      361 caggtattac ggttaaaggt acccaggatg cccgtgaact gggcaacgct tttagcgctg
      421 acatccctga tttcgatctt tattccagta ataccgtccg gaccattcag tcggcgacct
      481 ggttttccgc gggtaaaaag ctgacggtag ataaacgact tcttcagtgc ggtaatgaga
      541 tttatagtgc aattaaggac ttacaaagca aagcgcctga taaaaatatc gttattttca
      601 cccataatca ttgcctgaca tatattgcta aaaataagcg tgatgcgaca tttaaacctg
      661 attatctgga tggtttagtc atgcatgtgg aaaaaggcaa agtttatctg gatggggaat
      721 tcgttaacca ctaaattttc aatctgacag ccagtgatgg ctgtcatcat tgttaaagaa
      781 gacctttcaa acgtaacgtt tttcgggtgg caacatttaa atcataatgc accagatctt
      841 caggttttac ccacgcgtag tcctgaaact cttcgtttat tttcacttct cggttggcag
      901 aaacgcagtc aaaaatcagg taaatcatat aaatctcttc cttgcgacca tctgcatacg
      961 tcttggtgcg aatatcatcg ctgaaggtcc acggcgtgat ttctgtcaaa agcagctgtt
     1021 ctcccagttc ttcgcgaatt tcgcggcgta gtgcctcttc aattcgttcg ccagactcca
     1081 cgccgccacc cgaaagcgcc cattgaccgg ggaaaacgcc gcgatcgtcg gccattttac
     1141 acagcaaata agcaccatca ttttgaatca aagggcatac aatagtccgt tgtcgcatag
     1201 gggtattcct tataa
//
LOCUS       DAZKIV010000363         1203 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZKIV010000363 DAZKIV010000000
VERSION     DAZKIV010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.366"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(167..>1203)
                     /locus_tag="ACHOT6_005009"
     CDS             complement(167..>1203)
                     /locus_tag="ACHOT6_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP9950031.1"
                     /translation="SPDCSHIKKICFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQ
                     HIVFTLPCQYWSLVFHNRWLLAEMSRIAADVIQEICRQADVVPGIFTVIHTWGRDQQW
                     HPHIHLSTTTGGVTSDHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVIPDALAAEG
                     SSKRDWNRFLDSHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPMSRLEHYAGQDE
                     IGLRYNSHRTKREEYLVMSGDEFMERFSWHVADKGFRMVRYYGFLSPVKRRLLEDVVY
                     VITETVRKTAMQIRWRGMYQRLLKVDPLKCILCILCGGQMRFTGLKRGYRLTELVLMH
                     EPLAQQRVCG"
BASE COUNT          278 a          370 c          274 g          281 t
ORIGIN      
        1 aaaaagatta aaaaaacacc caaaaacgga tgtttattca acactgcctg cgtgccatga
       61 gtgcaccgcc gtcaattaaa tggatcttat cgattgatac tgcatatcac tgatggtttt
      121 ttaccccatt cccctgaaaa tggacgcaac ttctccgatg cggctctcag ccgcacaccc
      181 gctgttgcgc cagtggctca tgcatcagga ccagctctgt cagacggtag ccccgcttca
      241 gccccgtaaa acgcatctga cctccgcaca ggatgcacag gatgcacttc agcgggtcaa
      301 ccttcagtaa ccgctgatac atccctctcc acctgatttg catcgccgtc tttctcaccg
      361 tctccgttat gacgtacaca acatcttcca gtaaccggcg cttcaccgga ctcaggaaac
      421 cgtagtacct caccatacgg aaccccttat ccgccacatg ccaggagaac ctttccataa
      481 actcatcacc actcatcacc aggtattctt cccgttttgt ccggtgactg ttgtaacgca
      541 gaccaatttc atcctgacca gcatagtgct ccagacggct catcggcacc ggcggttttt
      601 tcaggtaaga gccgaagtac accgccacat gtgtggcgtt atccatcacc cgggatacgt
      661 tgacattcca gccccgccgg taatgactgt ccaggaagcg attccagtcc cgtttactgc
      721 ttccttctgc tgccagcgca tccggtatca ccaggtccgg atatttccgt gacagtaacc
      781 gcgttatccg gtaacgccac atactcatca ccttacgggc gtaaaaatga aggtttttcc
      841 aggtgtggtc tgatgtcacg ccgccggtcg ttgtcgacag gtgaatgtgc ggatgccact
      901 gctggtcacg tccccatgtg tggatcaccg tgaatatccc cggcaccaca tctgcctggc
      961 ggcagatttc ctgtatcaca tccgcagcaa tgcggctcat ctctgccagt aaccaccggt
     1021 tgtggaacac cagggaccag tactggcagg gaagtgtgaa cacaatatgc tgccacggac
     1081 agtcgggaac cagactcagc agatactgta tccactgtgc gccagccttc actccgcagt
     1141 gcgggcagga gcgactttta caccggaagc agatcttttt tatgtggctg cagtccggag
     1201 atg
//
LOCUS       DAZKIV010000364         1190 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZKIV010000364 DAZKIV010000000
VERSION     DAZKIV010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.367"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..183)
                     /gene="ynfU"
                     /locus_tag="ACHOT6_005010"
     CDS             complement(13..183)
                     /gene="ynfU"
                     /locus_tag="ACHOT6_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding protein YnfU"
                     /protein_id="HFP9950032.1"
                     /translation="MSERKDSKSRRNYLVKCSCPNCTQESEHSFSRVQKGALLICPHC
                     NKVFQTNLKAVA"
     gene            402..617
                     /locus_tag="ACHOT6_005011"
     CDS             402..617
                     /locus_tag="ACHOT6_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416074.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9950033.1"
                     /translation="MKSMDKLTTGIAYGTSAGNAGFWALQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDRRKVARRE"
     gene            622..957
                     /locus_tag="ACHOT6_005012"
                     /pseudo
     CDS             622..957
                     /locus_tag="ACHOT6_005012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
BASE COUNT          341 a          213 c          297 g          339 t
ORIGIN      
        1 gaataaaatc aatcacgcta cagctttaag atttgtctgg aatactttgt tgcaatgagg
       61 gcagatcaaa agggcacctt tttgtactct tgaaaaactg tgttctgact cttgggtgca
      121 gtttgggcag gaacatttaa cgagataatt acggcgtgat tttgagtctt tacgttctga
      181 cataggcttt tcctgtataa atggccgtat acagtacact aaatatgaaa acatatctcg
      241 tattattatt taatacatga ttttctttta aaataattac ccacattttt aatgtgtctg
      301 ttttttagcg ccgttgagaa caatgtttgc tataaaaact aacccataga ctccgatctt
      361 ttcaaacata ttgcaccatc tgtgtacatc ggggcgagga tatgaaatca atggataagt
      421 taacaacagg tatcgcctat ggcacatcgg ctggtaatgc tggtttctgg gcattgcagt
      481 tactcgataa agtaactccg tcacagtggg ctgcaatcgg tgtgctgggt agtctggttt
      541 ttggcctgct gacgtatctg acaaaccttt atttcaagat taaagaagac aggcgtaagg
      601 ttgcgagaag agagtaatcc aatgacacaa gactatgaac tggttgtgaa aggagtccgc
      661 aattttgaga ataaagtggc ggtaactgta gccttacggg acaaaaaacg ctttgacggt
      721 gaaatttttg acctggacat ctcgctggac cgtgttgaag gtgccgcgct tgagttttat
      781 gaggcagcag cgagaaggag catcagacag gtcttcctgg atgttgctgc cgggttatgt
      841 gaaggggatg agctgtcgcc ggaaaagcgc cccgtaattt tagaggcgca gaatgtgtgg
      901 ataacctaca aaggaaagct accgggaaga attactggtt ctctgaagac gccaccgacg
      961 gcattgcggt cagaaaaaga tgatattgaa tcgcccattg aaaaactgga gggcagcgtc
     1021 gctgatctga ataaaaaatt gtcggtgctg attccttctg aagatgaaaa aaaacgccgc
     1081 gatgagcagt ttgcggcgtt ttacgattat tgcattgaag ttactcgcag gaattttgtg
     1141 aagatttttg aggagggtaa atctcttcag taagcttaat ggcggacgct
//
LOCUS       DAZKIV010000365         1183 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZKIV010000365 DAZKIV010000000
VERSION     DAZKIV010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1183
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.368"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            61..387
                     /locus_tag="ACHOT6_005013"
                     /pseudo
     CDS             61..387
                     /locus_tag="ACHOT6_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(528..>1183)
                     /locus_tag="ACHOT6_005014"
     CDS             complement(528..>1183)
                     /locus_tag="ACHOT6_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFP9950034.1"
                     /translation="NKEDDFLYATQNSPAQLFKITKSGDIMDRAPLPFISDAETIEHI
                     QGNIFAAVDENTSELFFFTVTKDMHISFRNKIQLEKFNKKNRGFEGLAWKADDRMLFV
                     AKERRPSKIFIYQLSPDLLSVKQATIPEELNDIRINDISGLAFNNESLMILSDESRNL
                     LKFNLTEMSFIEMLDLTKGNHSLTSDLPQPEGIVTLSDESIYVASEPDILAKFVPNK"
BASE COUNT          341 a          213 c          268 g          361 t
ORIGIN      
        1 gtattaagac tatcacttat ttaagtgata ttggttgtct ggaaataagg gggcaagaac
       61 aagtggctga cggataatgg ttcatgctac cgggcgaatg aaacacgtca gttcgccagg
      121 atgttgggac ttgaaccgaa gaacacggca gtgcggagtc cggagagtag cggaatagca
      181 gagagcttcg tgaaaacgat aaagcgtgat tacataagta tcatgcccaa accagacggg
      241 ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg gcatccgcat
      301 agtgcactgg gttatcgctc gccacgggaa tatctgcggc agcgggcttg taatgggtta
      361 agtgataaca gatgtctgga aatatagggg caaatccact ggttgataat agctgatgtc
      421 cttttcagct attaatgtgt ttattttagc gtaaaattat actcgtattt ttataacttt
      481 atgagattgc cagatttggt cgataaataa taatgccagt gacagactta cttattaggg
      541 acaaatttcg cgaggatatc aggttcactg gcaacataaa tgctttcatc agacagagtt
      601 acaattcctt caggttgggg caaatcactg gtcagagaat gatttccttt tgtcaggtca
      661 agcatctcaa tgaaagacat ctctgtcagg ttaaacttga gtaagttacg cgattcatcg
      721 gacagaatca tcagactttc attattaaag gccaggccgg atatatcgtt aatacgtata
      781 tcatttagtt cctctggaat agttgcttgc tttacactca gcaggtctgg agaaagttga
      841 tatataaata ttttacttgg gcgtctttct tttgcgacaa acagcattcg atcatctgct
      901 ttccatgcca gcccttcaaa tcctctattc tttttattga atttttctaa ctgaatttta
      961 tttcgaaagg agatatgcat atcttttgta actgtaaaaa agaatagttc agaagtgttt
     1021 tcgtctactg cagcaaaaat gttcccttgt atgtgttcaa ttgtttcagc atcagagata
     1081 aaaggcagcg gggctctgtc catgatgtcg ccacttttgg ttattttaaa tagctgtgca
     1141 gggctattct gggtggcata caggaaatca tcctctttat tat
//
LOCUS       DAZKIV010000366         1181 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZKIV010000366 DAZKIV010000000
VERSION     DAZKIV010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.369"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(49..1179)
                     /locus_tag="ACHOT6_005015"
     CDS             complement(49..1179)
                     /locus_tag="ACHOT6_005015"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFP9950035.1"
                     /translation="MIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIKNPGY
                     SNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESVIVVH
                     NSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLANADIE
                     IVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKLAAAH
                     KNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPLADLV
                     VIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSISSDI
                     LKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
BASE COUNT          369 a          236 c          198 g          378 t
ORIGIN      
        1 gaaaaacaag taaaaaataa aaaaggctgc ataatgcggc ctttttattt attcaaacca
       61 attatgaata acctcttcga atctttgagt tacgccatcc caactgtagt gttcaaacac
      121 aaagtcctga ccttgctttg caactgctgt taattcagga ttcgctaacg tttttaaaat
      181 atcgctgctt atggagtctg cagtcatagg ttcttttaaa tggaatcccg tagtattttc
      241 tttgacaaat tccgtcatcc ccccacgcgt actcactaaa acaggcttac cagcacccat
      301 agcttcaatt gccaccatgc aaaatggttc ctggaactgg gaaggaatga cgactaaatc
      361 tgccagtggg taataacaat gcatcttttc aggcggtatg cttcctaaca taatgcaacg
      421 atccttcaaa cgttttgcaa tttccctgac attgcgttga tatgcacctt tgtcactttt
      481 actcatttct gtataatcac caatcacaac taattttaaa tttttgtgag cagcggccaa
      541 tttttcgaat gattgcatca acagcaatat gcctttgtca gggacgatac gcccagcata
      601 aaaaataatt ttctcttcag gggagatatt aagttcgctt ctagtgatcg gctgaaaatt
      661 agattgatag gtttctaaat caattccatt tggtacaatc tcaatatctg catttgctaa
      721 ataagattgg taatactttt tcaagtataa actaggcact atcattttaa cattttgctc
      781 aagcaatttt ggttcaaatg cattatgcat atgtataacc actcttgcct gaggagctcg
      841 cttacgaatt tgagtgtata gcttcatgct attatgaact acaataacac tttctttcgt
      901 tataggaaaa tcatgtgcaa tattcaatat tctctgtgaa tatggaagag gatcaagtct
      961 tgtccatttt tgaaataacc gcttatatat gcgactgaat ccaaccctat gaatactaca
     1021 attatcatta acaaaggtgt aatttgagta tcctggattt ttgatacaaa caatacgatt
     1081 tggaaaattg gtacgctgag caacctgata catccacgtt tcaacagcag ccgctccgcg
     1141 aggaggaatt gaaaaaatag gagtaaccgt aaatattatt t
//
LOCUS       DAZKIV010000367         1180 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZKIV010000367 DAZKIV010000000
VERSION     DAZKIV010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.370"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1180
                     /locus_tag="ACHOT6_005016"
     CDS             <1..>1180
                     /locus_tag="ACHOT6_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HFP9950036.1"
                     /translation="SNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSGAYQKLTIGSD
                     WGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGITNKASSYGVP
                     PDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIATCNQLLKSLYY
                     DVEVLFDWINDGQKFESIHPKGIIPEKIITKRSERTDEIIRILEYIDNTLITFCNEEI
                     IKCQIAIQSIISKNNNPSEFRIMATGHAHLDIAWMWPLREGRRKAIRTFATALANIEK
                     YPDYIFGASQYQLFHWIKKDYPYFFEKLKKQIAAGRFELQGCFWVECDLNLVSGESLI
                     RQIMHGTRFTLQNFSKKINYVWQPDVFGFPATLPQILKKSGINYIASQKLSQNKINKF
                     "
BASE COUNT          377 a          225 c          241 g          337 t
ORIGIN      
        1 tgtcgaataa aataggtcag cgtgtttata ctgttgtctc tgaaatgtcg attgaagcat
       61 ggataacaaa agaaccgcag ccttttgttt atcgccgtag cggcgcttac cagaaactca
      121 ccattggctc agactggggt caactgtttg actgtgcctg gatgcgagtt tatggtaaac
      181 gtccagcaga taaatttaga cacaaactcg tcgctctcgt tgatatcggt ggcgaaggtc
      241 ttattgtgga taagaatggt tcacccatct gcggcatcac gaataaagca tcatcttatg
      301 gcgtaccgcc agataaacca ggtaaatggg tggttgacct ttctctcgtt agtgaaaatg
      361 acgaggtcga gttttggatt gatgctgcgt gcaacgattt atttggctat gtcactaatg
      421 gagggattat tactgatgtt catatcgcga catgtaatca actactgaaa tccctttatt
      481 atgatgttga agtgcttttc gactggatta atgatggtca gaagtttgaa tccatacatc
      541 ctaaaggtat tatcccagaa aaaattatca ccaaaagaag cgagcgtact gacgaaatca
      601 taagaattct cgaatatatt gataatacgt taattacctt ttgcaatgaa gaaataatta
      661 aatgccaaat tgcaatccaa tccataatta gcaaaaacaa taatccttca gagttcagga
      721 ttatggctac tggtcatgcc catttagata tagcgtggat gtggccttta agagaaggac
      781 gcagaaaagc aataagaaca tttgccaccg ccctggcaaa cattgaaaaa tatcctgatt
      841 acatttttgg cgctagtcag taccaacttt ttcactggat aaaaaaagat tatccctatt
      901 tcttcgaaaa gttaaaaaaa caaatcgccg caggccgctt tgagttacaa ggttgctttt
      961 gggtcgaatg cgatttaaat ctggtaagtg gtgaatcgct gataaggcaa atcatgcatg
     1021 ggacacgatt cacactgcag aatttttcaa agaagataaa ttatgtctgg caaccggatg
     1081 tctttggatt ccccgccacg cttccacaaa ttctcaaaaa atcaggcatt aactatattg
     1141 catcgcaaaa gctctcacaa aacaagatta ataaatttaa
//
LOCUS       DAZKIV010000368         1162 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZKIV010000368 DAZKIV010000000
VERSION     DAZKIV010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.371"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(43..>1162)
                     /locus_tag="ACHOT6_005017"
     CDS             complement(43..>1162)
                     /locus_tag="ACHOT6_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFP9950037.1"
                     /translation="TWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREK
                     AQCRSLAEKLQQEGSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTV
                     ERLAAQIEPVYLPERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHH
                     LHQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVI
                     DPMGLDAIENMTSGGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAM
                     RHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAK
                     CSDACIEKYNTGQLFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          260 a          349 c          241 g          312 t
ORIGIN      
        1 acaaataaac aaagggtaat taaaaaaatg acaagtttgc ctctaataat ttgaagcatc
       61 tgaactccct tgctttgctt ttattggatt atctgctttt ataccatcta acccgaagag
      121 ttgcccagtg ttatattttt caatacatgc atcgctacat ttggcagaaa aatcgccaca
      181 agcaataccg acagtgttat ttttaagatc catgattctt tctttagctg gctgaccttg
      241 tctatcccct gccgcctcat gatttttacc tatcacgtca gctattgttg atccaaatgt
      301 cttagtcatt cgacacatca agtaacaatg tctcattgca tcggcggccc cattatgagc
      361 tccgccaaca atagcatcca tatcataacc gaactggtaa gcactgttag taatgctgtt
      421 tgcagcaatc aatccaggta caccagatac ggcataaatt agtccacctg atgtcatatt
      481 ctcaatcgca tctaacccca ttgggtctat cacttgtatg ggattcaacg gatactgata
      541 catattccat ccacctctca acccaatcgg gtccggggtg atataccgcc cctgcaacgg
      601 atcgtagtac cggtgacggt tatagtacag ccccgactcc ttatcatact gctgccccgg
      661 cagccggtac ggctggtgca ggtgatgcgg gttctcctca ttaagctggt tgccccattc
      721 atcatattcc gcgctccacg ccgtattgcc gtcttcactg ataagcgcca gcggcaggcc
      781 ccggtggtca cagtgataaa gatgtatttt tcgctcaggc aaataaaccg gctctatctg
      841 tgccgccagc cgctccaccg tcagcccgca ctgcgcaagc cactggcggc tttcactgct
      901 cacgcagttt gcccggattt ccccctccag cctgtccagc agccccacca gttcagccgg
      961 gaacaccaca ccgtgaccgt cttcgctccc ttcctgctgt aacttctccg ccaggctgcg
     1021 gcactgcgct ttctcccgtt caccgttgtc tgtttcgatg cggatgagcg gcgcgaagct
     1081 ccccggctgg tataccgtct ggatacgcgt ggtgtcggtc tgtaccgtcg tcagcctgtc
     1141 accgtcccac ccgtaccagg tc
//
LOCUS       DAZKIV010000369         1156 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZKIV010000369 DAZKIV010000000
VERSION     DAZKIV010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1156
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.372"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..277
                     /locus_tag="ACHOT6_005018"
     CDS             <1..277
                     /locus_tag="ACHOT6_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9950038.1"
                     /translation="VSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGFVSSK
                     KIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
     gene            complement(370..729)
                     /locus_tag="ACHOT6_005019"
     CDS             complement(370..729)
                     /locus_tag="ACHOT6_005019"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFP9950039.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          364 a          222 c          203 g          367 t
ORIGIN      
        1 tgtctctgac catggagaat ctctgggaga aaatggatta tacttacatg gaacaccata
       61 taatgttgct ccagctgagc aaacgcatgt gcctcttatt acctggatgt ctccggggtt
      121 cgtttcctct aaaaaaattg atttgaattg tttgtcagaa catgctctaa acagaacagt
      181 aagtcatgat aatatatttt catcgttact ggggttatgg aatgtaaata cttcagttta
      241 taataaagaa gatgatatta tatcctcctg cagatagata ctgacatttt tctgaggata
      301 agaggtgtat acctcttatc cttaagtaaa tattaaccag gtaactgtga ataatttaga
      361 tgttgggcgt tatgaaagta ataaaactcc ccatataatg atagcaacta aaatcgtaat
      421 caacactgcg gctgaaccca tatcttttgc tctgcctgat aattcgtgat gttctgaacc
      481 aattctgtca acaactgctt caattgcact gttaattatt tcaacaataa ttaccatcat
      541 tgccgaacca atgagtaata ctcgagtaac agagtcaaca tccagccaaa aggatattat
      601 cagtgctgac agaaccagaa tgcactcctg acgaaaggct gcttcatagc gccatgccgc
      661 acataagcct ttccatgaat aacccgctgc tttaataatt ctggtaacac cagtggtcgt
      721 attagccata aatacctctt atttaattat atgtctgaat atgcattata aaatatcagt
      781 taacattaac aggattttgt aataagccac atttattctt tcgggaatta aaaaacataa
      841 cgaaattttg cctcattctg catttcattg aaatagtatg tttctcatcg aaagcaagtt
      901 tgtcaatata aatttgtaac agaaatgact ttccggggat aaacaaaact attcatggca
      961 atggctttct ctgtcgccaa catggataca ttgttccgga taaagcactg atgtcattga
     1021 ctgacgcaga ctatcaggcc atctgatgct ttcttttcct tcaccggtat tgatgatatg
     1081 ccttatgaag ggggacttat tcctgtaacc caatcacagg cactgtgatt actggtttat
     1141 gattgatgct gatttg
//
LOCUS       DAZKIV010000370         1154 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZKIV010000370 DAZKIV010000000
VERSION     DAZKIV010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.373"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..63
                     /locus_tag="ACHOT6_005020"
     CDS             <1..63
                     /locus_tag="ACHOT6_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9950040.1"
                     /translation="VRPLQILRADGTWENIGGMK"
     gene            65..184
                     /locus_tag="ACHOT6_005021"
                     /pseudo
     CDS             65..184
                     /locus_tag="ACHOT6_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309586.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            380..529
                     /locus_tag="ACHOT6_005022"
                     /pseudo
     CDS             380..529
                     /locus_tag="ACHOT6_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            750..887
                     /locus_tag="ACHOT6_005023"
                     /pseudo
     CDS             750..887
                     /locus_tag="ACHOT6_005023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
BASE COUNT          324 a          186 c          320 g          324 t
ORIGIN      
        1 gtaagaccgc tgcaaatcct gcgtgctgac gggacgtggg aaaatattgg cgggatgaag
       61 taacccgaca gcttcacaaa accggagtcc ggctccggtt tttgttgtca tgtatagggg
      121 atatttgtta ttagagtgtg aagtaataaa catgttaata cgatggagtg aaggatgcct
      181 ttaaggagca gtgatatgtc catcttgttc cctggagggg gaatcataaa ttctgattgc
      241 gaccgaggca tggaacacta ctgtagttca gggaagttcg gcgatgaagc tcattcttgc
      301 gacgcgtaat tattatctgg aatatggttt gcgtttgtta ctgaaaggat gccgtgtaat
      361 tctggctcaa gaatttttta tgccggagaa tcgcagggtt attctggata gtaaagaatc
      421 ctggttaata atctgtgata gtcagttggg ccatttaatg cgcagcatgt ttcagggacg
      481 ccgttttatt cagctggatc tggaagcgtt gaaaggggga catgatatac ataatgctgt
      541 gcgtaacaga ttgtggacct ggaacaaaaa agcacgggca ctgacgatgt cagagatggt
      601 ggtgatgttt ggatatatct accgtcagtt gcgtccgtcc catctcgcca gtgagatgag
      661 ggtaaacata aaaacagtta acactttcct gtattcaggg ctggcgaaaa atgggctcaa
      721 aagaagtagt gtaaggttgc tggcaatttc tgagaacaag cgaatagggc attattccgg
      781 agcaggacag ggtaatcacg caggtggtga tactggatgc ggataaaaag ccgatacagt
      841 gcgtggtgag gccgctgcaa attcggtgtg ctgatgggac gagggaaaaa tttgtggatg
      901 acatttgttg tggacctttt agtctggagt tcaaattaaa acagggagct ttattatgcc
      961 attaacctca gatattaatt catcttcgtt ccatcttgga atggaatttg taaaaaaacg
     1021 ccacaggcat caggaatata agaaggtgtc tgtggttgac aggtttcaga tgacttgagg
     1081 gcgtctttat gtaactgaat ttttctgtgg agatgctgtc tgaaaatatt tgattaaaaa
     1141 cggaagctaa gtta
//
LOCUS       DAZKIV010000371         1151 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZKIV010000371 DAZKIV010000000
VERSION     DAZKIV010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.374"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..260)
                     /locus_tag="ACHOT6_005024"
     CDS             complement(<1..260)
                     /locus_tag="ACHOT6_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950041.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IGSMDVLRKAVFEGVNIIITHEPTFYSYADLEGDVLEFSWAR"
     gene            complement(283..1122)
                     /locus_tag="ACHOT6_005025"
     CDS             complement(283..1122)
                     /locus_tag="ACHOT6_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HFP9950042.1"
                     /translation="MNDIENYGNRYHVLQSFPMPQLASSREIRILLPASYYESDVSYP
                     VLYMHDGQNLFDKSVSFGPHVWDIPEAVDAFFPNSQYDGVIIVGIDNASSHGKFSRMD
                     EYSPWPRNTSFPLPGWDPDVDYSGGKGALYVDFIVNTLKNYIDSNFRTLPDRNNTAIA
                     GSSMGAYISLFAAILRLDVFSKVGVFSPALWFNDSAMLNFIQENNIFADLTVYLDVGT
                     LETSGMREDFPEVYISGAEKLCVSLRKQRNVTIDYHLWGGDTHSESAWAKRFPEMLKL
                     FYC"
BASE COUNT          386 a          254 c          199 g          312 t
ORIGIN      
        1 tttctggccc atgaaaactc aagaacgtca ccctccagat cagcataaga ataaaatgta
       61 ggctcatgag taataatgat attgacacct tcaaaaacag cctttcttaa aacatccatt
      121 gagccaatcc agcaggtccc aattttatta ataagtgtcg cctcagcacc aaaaataatt
      181 ttatcaacag ttggttgcga aacggcaacc attgaattta aaaaacaatt aagatctgcg
      241 gctgttttaa tatgatgcat tcaacatcct tcaccggttt catcagcaat aaaataattt
      301 caacatttct ggaaaacgtt ttgcccatgc agactcagag tgggtatcac ctccccatag
      361 atgataatct atagttacat tgcgttgttt acggagactc acgcataatt tttccgcgcc
      421 tgaaatatag acttcaggaa agtcttcgcg cataccactg gtttccagcg taccgacatc
      481 gagatataca gtaagatctg caaagatatt gttttcctga ataaaattca gcattgcgct
      541 gtcattgaac catagtgccg gcgaaaaaac gccaactttt gaaaaaacgt ctagacgaag
      601 aattgcggca aacaaactga tgtacgcacc catagaacta cctgctatcg ctgtattatt
      661 acgatctggg agagttcgaa aattgctgtc aatatagttc ttgagcgtat tcacaataaa
      721 atcaacatac aacgcacctt ttcctccact ataatccaca tcagggtccc agccaggtaa
      781 gggaaaagat gtgtttcgcg gccatggaga atactcatcc atacggctga atttaccatg
      841 agacgatgca ttatcaatcc ccacaatgat aacaccgtcg tattgggaat ttgggaaaaa
      901 agcatctacc gcctcaggta tatcccatac atgtggacca aaagatacag atttatcaaa
      961 taaattttgc ccatcgtgca tatacaatac agggtaactg acatcagatt cataatagga
     1021 cgcaggtagt aatattctta tttcgcgact acttgccaat tgtggcatcg gaaaggattg
     1081 caacacatga tatcgattcc catagttctc aatatcgttc ataatttaaa ttaacctcaa
     1141 taaacaaaag a
//
LOCUS       DAZKIV010000372         1140 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZKIV010000372 DAZKIV010000000
VERSION     DAZKIV010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1140
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.375"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1130
                     /locus_tag="ACHOT6_005026"
     CDS             <1..1130
                     /locus_tag="ACHOT6_005026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISEc20 family transposase"
                     /protein_id="HFP9950043.1"
                     /translation="HAVAINRSGKRLFDKALPNDENKLRSLISDLKQHGQILLVVDQP
                     ATIGALPVAVARSEGVLVGYLPGLAMRRIADLHAGEAKTDARDAAIIAEAARTLPHAL
                     RTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRGLLTQIHPALERVLGPRLDHPAV
                     LDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRLAADIAQALAEQTVVVPGTNAAA
                     VVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPVLTSMPGVGVRTAARLLTEVACR
                     AFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNKALKRALFLSAFAALRDPLSRAY
                     YTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQAS"
BASE COUNT          242 a          370 c          293 g          235 t
ORIGIN      
        1 atcacgctgt agccattaat cgttcaggta aacgcctgtt cgataaagca ttacccaacg
       61 acgaaaacaa actcaggtcg ctaatatctg acctgaaaca acatggtcag atactgctgg
      121 ttgttgatca gccagctacc atcggtgcgt tacctgtcgc cgttgcccgc tcagaaggag
      181 tccttgtcgg atacctccct ggactggcca tgcgccgcat agccgactta cacgccggtg
      241 aagctaaaac tgatgctcgt gacgctgcca tcattgccga agctgcccgt accctgcctc
      301 acgcgctacg cacgctgaaa ctggctgacg agcaaatcgc cgaactctcc atgctctgcg
      361 gcttcgatga tgatcttgcc gcacagacaa cgcaggccag caaccgtatc cgcggccttc
      421 tgacccagat acatccggca ctggagcgcg ttctcggtcc gagacttgat cacccggcgg
      481 tactcgatct tctccagcga tatccctcac cagaaaaact cgcttcgctg ggtgagaaga
      541 agctggcagc ccagctctgc aaacttgcgc ctcgtctggg taaacgcctt gcagcagaca
      601 tagctcaggc actggccgaa caaaccgtcg tcgttcccgg cacgaatgcc gctgccgtag
      661 tactgccacg tctggcactc cagctcatca cgctgcgtaa gcaaagagac gaggtggcgc
      721 ttgaggtaga acagcgagtt cttgctcacc ctctttaccc ggtcctgacc agtatgcccg
      781 gagtcggtgt caggaccgca gccagactcc tcaccgaggt cgcctgccgc gccttcgcct
      841 ctgccgcaca tctcgctgct tatgctggcc ttgcgccggt aactcggcga tccggctcgt
      901 caatacgcgg tgagcatccc tcgcgacggg gtaataaagc tctcaaacgg gcgttgttcc
      961 tgtcggcctt cgccgcgctc agggatccgc tctccagggc ttactacacc cgcaaaatga
     1021 gtcagggaaa acgacacaat caggcgctta tcgccctggc gagacgacgc tgcgacgttc
     1081 tgttcgccat gatgcgcgac gggacttttt ataccccgca ggcgtcataa catgcttgac
//
LOCUS       DAZKIV010000373         1137 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZKIV010000373 DAZKIV010000000
VERSION     DAZKIV010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1137
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.376"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..149)
                     /locus_tag="ACHOT6_005027"
     CDS             complement(<1..149)
                     /locus_tag="ACHOT6_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9950044.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTAGVV
                     EQNLSR"
     gene            complement(130..534)
                     /locus_tag="ACHOT6_005028"
     CDS             complement(130..534)
                     /locus_tag="ACHOT6_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9950045.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFLDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREEAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVSEDDVALMMASAGIPGGVRYVPAGW"
     gene            complement(561..992)
                     /locus_tag="ACHOT6_005029"
     CDS             complement(561..992)
                     /locus_tag="ACHOT6_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9950046.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHADTDSTEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(1006..>1137)
                     /locus_tag="ACHOT6_005030"
     CDS             complement(1006..>1137)
                     /locus_tag="ACHOT6_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HFP9950047.1"
                     /translation="SVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
BASE COUNT          261 a          361 c          261 g          254 t
ORIGIN      
        1 cgactcaggt tctgctcaac gacaccggcg gtttttctgg cgtcagtctc cagtccggaa
       61 aaatgacgcc ttacccggct catctgctca tcgaaacgga ccgcatccag actcaggtca
      121 ataacaagat caccaaccgg ctgggacata tctcacacct cccggaatcc ccgctgaagc
      181 catcattaat gcgacatcat cctcgctgac atccgcagac gataaaatct cacgccctcc
      241 gtccccacca aaccgtacgc ctccggcaag tcctgccgct ttctgcatca gcattttttc
      301 ctcatccggc atctccgtct gcgcttcctc acgccggggg gcaagcagac tgaaatcaga
      361 gggatgcata tccggatcgc aaaaaaacag gctgagtacg gcgtacgtca gcccggaaaa
      421 atgcatatcc agttgggtat cgagaaaata atgcgtacgg taaaaatgtc gccagtcggc
      481 atattcggtg gatgtcatcc cggcaagcat ggcgcgccag tcgggtctcc ccatctcacg
      541 cgccagtctg agggcaaaat tcagctcgcc gtcgaagact ttcccgcaga aaaatcatca
      601 tcagtcagcg cgttattttt cgccacttcg gtgctgtcag tatccgcatg aacagccccg
      661 ctcatcccgg acagacgcaa caccacatct tccgcccggg caatggcatc cgcaggccag
      721 gtggtgagca cctcctgttc gatctgcatc acagcctcat tcattgacgg tgatgccgtt
      781 ttctgcggat ggttatgcca cagggacatc gccaccagaa acgcgccggt tctgacgaga
      841 tcttccacgc ttacctgcag gttgccgctg gattctgcct gttctgcacg ccgtttcagg
      901 agggcaagat gctcaatacg ctgcagcgca gacaattcgg aaagcgtgac ggacacaccg
      961 ttatattcaa attgttctgt tttcagaaac atgtattacc tccgtttacc ctgcagcgcc
     1021 cgcttcagta acggtgactt cagccactgc ggcgaactga ccatttccgc tcaccacagg
     1081 gatctgcacc ttacctgtcg ccacgccgtt taccgtaatt gtcatatctt tcacact
//
LOCUS       DAZKIV010000374         1132 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZKIV010000374 DAZKIV010000000
VERSION     DAZKIV010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.377"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..583
                     /locus_tag="ACHOT6_005031"
                     /pseudo
     CDS             1..583
                     /locus_tag="ACHOT6_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
     gene            845..>1132
                     /locus_tag="ACHOT6_005032"
     CDS             845..>1132
                     /locus_tag="ACHOT6_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950048.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQKERFQQSIQDDKISFTIQTNHLEKYSMLSGMR"
BASE COUNT          403 a          187 c          208 g          334 t
ORIGIN      
        1 aaaatgtcat cgttctggct ggaaatcatg aaatcaattt taatggtaac tatactgcac
       61 gactagcaaa tcataaactc tctgccggtg atacgtataa cttaattaaa acgctcgatg
      121 tttgtcatta tgactcagaa acgaaagttt taaccagtca tcacggaata ataagaaatg
      181 aagaaaatgc atgttattgt ctcggtgctt tgcaggttcc atttaatcag atgaaagacc
      241 caattgatcc tgaggaatta gcaaatatat ttaataaaaa acataaacaa cacatggatg
      301 atcgttttat ccatctaatt agaagcaatg ctatagcatc cacacctgta tatgacaatt
      361 attttaataa cactactgct ttcagaccaa aacctgaaga tatcttcaaa tgtggacaga
      421 cgctcaaaaa actaaacaga aatatggcca ttatggatta ggggtagatc agcatcaaaa
      481 aattgataat tataccatgg ggctaaattc ctggaagata gctcctaacg aacgtggtga
      541 taaaaaaggt gtcccagggc ttagttgttt tcaaccacat taaaactcca tgagtgctgt
      601 ggtatatctt tttaatcctg ccagtatttc ataggttttc ttaatcgatt gtagaggcat
      661 gttctgatga cgcctctaca atctttaggg actttctctg ctatgagata agaaatttca
      721 tattatgctt taaggctgaa tgatttgtgc aaaatataat tatttttcca tcgaacatga
      781 ggtgtatgtc ataagataaa atgttaacta tgtattcctc gcggtaaata atatgaggta
      841 aaagatgaaa attggaactg tggcaggcac taacgacagt acaacgacaa tcgcaacaaa
      901 tgatatggtg caagagcatg ttaccaactt cactaaagaa ctgtttgggt atattgcaaa
      961 tggtataggt gatgatatat caagtattgc caggacaatg ctcggtgaag ttgtagaaaa
     1021 aattgatgat tggcaaaaag aacgtttcca acaatctatt caagatgaca aaatttcatt
     1081 taccatacaa acgaatcatt tagaaaaata tagtatgcta tcgggcatgc gc
//
LOCUS       DAZKIV010000375         1112 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZKIV010000375 DAZKIV010000000
VERSION     DAZKIV010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.378"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(509..>1112)
                     /locus_tag="ACHOT6_005033"
     CDS             complement(509..>1112)
                     /locus_tag="ACHOT6_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9950049.1"
                     /translation="YIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIRNTTGFCL
                     PQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSKQSLISRC
                     YNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNALSAIQTT
                     VKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          349 a          213 c          197 g          353 t
ORIGIN      
        1 gggatctact tcacaattta aatgagaaac taaaacttac atcttgaaat aatctcattg
       61 attggatgaa tatttgtagt gcagtgacat catttttaaa taatagttca aataaaaggg
      121 ctcacgatga aaaaattaac agtggcaatt tctgctgtag ctgcatctta acgtataaac
      181 tagatttaat aacaacgaac tagatttaat aacaacgata tccgtaccta tccggatatc
      241 taatgtcata agcatccctt taattatccc ctcaacctct tgtaactttc ccactcttga
      301 actccacata gtccaaacgc cggatgcaaa aaaatcacac gtgcatttct gaaattgtga
      361 tggaggaaga tagtatcaat tatatgtgct tataaacgtc cccgattagc gcagaagtca
      421 ccggattgtt caggctccaa taggttgagt atgccgtatt tattttccaa aataaaaatc
      481 gatgctgttg ctccttcata aaatactttt actctctgga cgcttttctt agctctcttg
      541 gtgttatctt aaaacgatta tggaaccttt cagtaaaacg agaaggacac ttgtaaccat
      601 tttccctggc aatctcgctg atgggtttta ctgtcgtttg tatagcagac aacgcattat
      661 ttaacctcac gtcgtccaat atactttgaa aacttactcc ctcgcttgcc agacggcgat
      721 gcaatgtgga aacagaaatg tagagatatc tcgctacctt gtttgctgtc cattttgttc
      781 caggttcgga tagcattagg ttataacaac gacttattaa tgattgttta ctatctgata
      841 aaagtaaaca attaacatga tgtgctccta atgaaagtag aacgcccatt gctaagtgtt
      901 cctgaatttg cgttgagaag cctcgggaaa tagatgtttt tagttgctcc caacaatata
      961 ttaactcggg attctgaggt aagcagaaac ctgtcgtgtt acgaatttga tcggttatcg
     1021 cataaagatt ttggaacttt tcaattagat caatgggata ataaagcatt tctgcaagat
     1081 aaagacctgc ttctgggtaa ttcgcaatat aa
//
LOCUS       DAZKIV010000376         1102 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZKIV010000376 DAZKIV010000000
VERSION     DAZKIV010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.379"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..198
                     /locus_tag="ACHOT6_005034"
     CDS             <1..198
                     /locus_tag="ACHOT6_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HFP9950050.1"
                     /translation="ILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFNICPEKV
                     AEYLSEFSGKHFLDVYNYYSN"
     gene            381..566
                     /locus_tag="ACHOT6_005035"
     CDS             381..566
                     /locus_tag="ACHOT6_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFP9950051.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRNKKVIGTFIKKHIFY"
     gene            807..>1102
                     /gene="nleB"
                     /locus_tag="ACHOT6_005036"
     CDS             807..>1102
                     /gene="nleB"
                     /locus_tag="ACHOT6_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9950052.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYSLEPIDEKPP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTY"
BASE COUNT          389 a          183 c          201 g          329 t
ORIGIN      
        1 atcctagcat tagcaattga ttgcgagaga aataatgcaa acataattat tgatagtgag
       61 tacaagtcaa ataaggcagt aaaggaatat gtagaaattt taaaagaatt caatatatgc
      121 ccagaaaagg tagcagaata cctaagcgag ttcagcggaa aacatttcct cgatgtatat
      181 aattattatt ccaattgaaa aaaaacataa agtatctatc acggcactct cagttgagta
      241 tcacaggcac atgtatttta gccccctgag tctacagaca accaatatta cttaaataag
      301 tgataatctt aatgctgttt tttagaacag atgattgata tttttgggcc ggagaaacgc
      361 agacaggctt tgttgaataa atcgagcttt tgctgagttt aaggatcaga tcactcattt
      421 tcctgcaaaa gctcattggg cgaaacaatc tgactcagag aacccggatt aaacgtctgg
      481 cccgcagaac catctatttc tcacgcactg tagtacgtaa taaaaaagtc atcggtactt
      541 ttatcaaaaa acatatattc tattaattat tgccatcacc tttttcttat tgacaacatt
      601 actctgctga ttgttaaaat ctaacctgta ttcagggtta cgattctata caatagaaca
      661 gggaggggaa aggatgtcat cgttaatgag actttttgtt aatgtcaaca atgctatgaa
      721 tttattttag aaaaacaatt aagctgacgt agtgttccgt ctaaatgacg gggcgatatt
      781 aacatgatta gaacaagagg aattttatgt tatcttcatt aaatgtcctt caatccagct
      841 tcagaggaaa gacagcttta tcaaatagta cacttctcca gaaagtttct tttgctggaa
      901 aagaatattc tctggaacct attgatgaaa aaccccctat tctttttcag tggtttgaag
      961 caaggccaga gcgatacgaa aaaggagaag taccaatatt gaataccaaa gaacatccgt
     1021 atttgagcaa tattataaat gctgcaaaaa tagaaaatga gcgtataatc ggtgtgctgg
     1081 tagatggaaa ttttacttat ga
//
LOCUS       DAZKIV010000377         1100 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZKIV010000377 DAZKIV010000000
VERSION     DAZKIV010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.380"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..808
                     /locus_tag="ACHOT6_005037"
     CDS             65..808
                     /locus_tag="ACHOT6_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFP9950053.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYLPCVNISAEPE
                     AYFRIAPEDWLRAEMSGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVSRGDIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGTEMPDFHREDDWWRNGQNLYLDNMEGT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGIYNDLAAASACM"
     gene            805..>1100
                     /locus_tag="ACHOT6_005038"
     CDS             805..>1100
                     /locus_tag="ACHOT6_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9950054.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIAGDDTAPEAVYAR
                     LHEQLGEGTVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMALWGSALAAG"
BASE COUNT          208 a          296 c          362 g          234 t
ORIGIN      
        1 ggcatggtgg ctaattttgg cggtttcctt tccattaata aactttcgca gtaaatcccg
       61 gtttatgaca cagactgaat cagcgattct ggcgcatgcc cggcggtgtg cgcctgcgga
      121 gtcgtgcggc ttcgtgataa gcacgccgga gggggagcgg tatctgccgt gtgtgaatat
      181 ttccgcagag ccggaggcgt attttcgtat cgcaccggaa gactggctgc gggcagagat
      241 gtcgggggag attgtggcgc tggttcacag tcatcccggt ggtctgccct ggctgagcga
      301 ggctgaccgg cggctgcaga taaaaagcgc actgccctgg tggctggtca gccggggtga
      361 cattcacaaa ttccgctgcg ttccgcacct gaccggacgg cgctttgaac acggggtgac
      421 ggactgttac accctgttcc gggatgcgta ccatctggcg gggactgaaa tgccggattt
      481 tcatcgcgag gatgactggt ggcgcaacgg ccagaacctg tacctggaca atatggaggg
      541 aacgggcttt taccgggtgc ccctgtcctc tgcacaggcg ggcgatatcc tgctgtgctg
      601 ctttggcgca tcggtggcca atcatgccgc catatactgc ggcaacggtg aactgcttca
      661 ccatctgcct gaacaactga gtaaacggga gaggtattcc gaaaaatggc aacgacgaac
      721 gcattctgtc tggcgtcacc gccactggca cgcatctgcc ttcacgggga tttacaacga
      781 tttggccgcc gcctcagcct gtatgtgaac acggcagcgg aagccatccg ggcgctgtcg
      841 ttacaggtgc ccggattccg ccgtcagatg aacgaaggct ggtaccagat acgtattgcc
      901 ggtgatgaca cggcaccgga ggcggtgtac gcccgtcttc acgaacagct gggtgaggga
      961 acggtcatcc acattgtgcc gcgactggcc ggggccggaa agggtggact gcagattgtg
     1021 ctgggggcgg cagccatcgt gggctctttc ttcactgccg gggcatcgat ggcgttatgg
     1081 ggttcagccc tggcagccgg
//
LOCUS       DAZKIV010000378         1086 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZKIV010000378 DAZKIV010000000
VERSION     DAZKIV010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1086
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.381"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..741
                     /locus_tag="ACHOT6_005039"
     CDS             <1..741
                     /locus_tag="ACHOT6_005039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9950055.1"
                     /translation="QPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAPDIVWDFRL
                     PRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAG
                     QVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCF
                     KLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLL
                     EDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          260 a          273 c          261 g          292 t
ORIGIN      
        1 caaccgctgc acgatgcgct ggataaattc caggccgaca ccggtatcga catcgacatg
       61 cacatcgacg ctgccagcgg tggcttcctg gcaccgttcg tcgccccgga tatcgtctgg
      121 gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag gccataaatt cggtctggct
      181 ccgctgggct gcggctgggt tatctggcgt gacgaagaag cgctgccgca ggaactggtg
      241 ttcaacgttg actacctggg tggtcaaatt ggtacttttg ccatcaactt ctcccgcccg
      301 gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc tcggtcgtga aggctatacc
      361 aaagtacaga acgcctctta ccaggttgcc gcttatctgg cggatgaaat cgccaaactg
      421 gggccgtatg agttcatctg tacgggtcgc ccggacgaag gcatcccggc ggtttgcttc
      481 aaactgaaag atggtgaaga tccgggatac accctgtacg acctctctga acgtctgcgt
      541 ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg aagccaccga catcgtggtg
      601 atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg ctgaactgtt gctggaagac
      661 tacaaagcct ccctgaaata tctcagcgat cacccgaaac tgcagggtat tgcccagcag
      721 aacagcttta aacacacctg ataacataac gttgtaaaaa ccgaatgccc agcctttaaa
      781 aaaacagctg ggcattcggt tgcttattaa tcgcaataat atattggctg tctattcatc
      841 gtgttgataa gatatatgtt atatgaatgt ttattaaatg tagcgattgc ctttaatgtc
      901 acgaaaaggg attatttgct tgctattaat ttcactgcgt tcttaaacac gtttttgtat
      961 tataaatcca gtcatcctgc ccgtagaaca tcacacatta tcatcctgtt ctcccgcttc
     1021 gtttaaattt atttatcaat caatttgact taagagggcg gcgtgctaca ttaattaaca
     1081 gtaata
//
LOCUS       DAZKIV010000379         1050 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZKIV010000379 DAZKIV010000000
VERSION     DAZKIV010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.382"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..186
                     /locus_tag="ACHOT6_005040"
     CDS             <1..186
                     /locus_tag="ACHOT6_005040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950056.1"
                     /translation="QSTTDAQGVFSSVIDMPAGQGTLTLTFTVSSSGANNWTPTTSIS
                     DLLVVVMKKSTAGISIS"
     gene            253..852
                     /locus_tag="ACHOT6_005041"
     CDS             253..852
                     /locus_tag="ACHOT6_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9950057.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLCVTDSKGKRHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDVAYEGSGSGDWRTDGFIVGVGYKF"
     gene            912..>1050
                     /locus_tag="ACHOT6_005042"
     CDS             912..>1050
                     /locus_tag="ACHOT6_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFP9950058.1"
                     /translation="MAAVQISGVLKDGAGKPIQNCTIQLKARRTSSTVVVNTVASENP
                     DE"
BASE COUNT          255 a          246 c          308 g          241 t
ORIGIN      
        1 cagtccacga cggatgctca gggcgtattt tcctcagtta tagatatgcc tgccggacag
       61 gggacgctga cactgacatt caccgtatct tcatcaggag cgaataactg gacaccaaca
      121 accagtatca gcgatctgct ggttgtggtg atgaaaaaat ccacagcagg tatcagtatc
      181 agctgaattt tataacccag aacgggcgtc agaaatgacg ccttttttat tgcagaaaag
      241 cgagaggtaa ttatgcgtaa actttatgcc gccattttgt ccgcagccat ttgtctggcc
      301 gtatccggtg cgcctgcatg ggcatctgaa catcagtcca cgctgagcgc ggggtatctt
      361 catgcctcga cgaacgttcc cggcagcgat gatctgaacg ggattaacgt gaaataccgt
      421 tatgagttta cggacacact ggggatggtg acgtcattca gctatgcagg agacaagaat
      481 cgccagatta cccgttacag cgatacccgc tggcatgaag attccgtgcg taaccgctgg
      541 ttcagcgtga tggcggggcc gtctgtacgc gtgaatgaat ggttcagcgc gtatgcgatg
      601 gcgggtgtgg cttacagccg tgtgtcgact ttttccgggg attatctctg cgtaactgac
      661 agcaagggga aaaggcacga cgtgctgacc ggaagtgatg acggtcgcca cagcaacacg
      721 tctctggcgt ggggagctgg cgtgcagttt aacccgaccg aatccgtggc cattgatgtc
      781 gcttatgaag gttccggcag tggtgactgg cgcactgacg gtttcatcgt gggtgtcggt
      841 tataaattct gattagtaac acagcgttat gacagcccgc cggttcaggc gggctttttt
      901 gtggagaggg tatggcagca gtacaaatat caggcgtgct gaaagatggt gcgggaaaac
      961 caatacagaa ctgcaccatt caactgaaag ccagacggac cagcagcacg gtggtggtga
     1021 acacggtggc ctctgaaaat ccggatgaag
//
LOCUS       DAZKIV010000380         1017 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZKIV010000380 DAZKIV010000000
VERSION     DAZKIV010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.383"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..>1017)
                     /locus_tag="ACHOT6_005043"
     CDS             complement(33..>1017)
                     /locus_tag="ACHOT6_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HFP9950059.1"
                     /translation="TGGTGSFGNTVLKRFLNTDVKRIIVFSRDEKKQEDMRIALANPK
                     LKFIIGDTRDYESVYRAMNGVDYVFHAAALKQVPSCEFYPMEAVKTNVNGTENVLNAA
                     IDCGVKRVVLLSTDKAVYPINAMGISKAMAEKVLIAKARRQDNDKTIMCATRYGNVMA
                     SRGSVIPLFFEKIKLNKPLTVTDPNMTRFLMSLEDSVDLVLHAFKNGRQGDIFVQKAP
                     ACTIGDLTEAIKQLCDSNIETKIIGTRHGEKLYESLVSREEMVKAEDMGRYYRIPADN
                     RDLNYDKYIIKGQIDINKLDDYTSHNTERLNVEQIKALLLTLKYVQKELGI"
BASE COUNT          301 a          209 c          154 g          353 t
ORIGIN      
        1 attattattg gatttgcaac ataacaattt atttaaatgc caagttcctt ttgaacatat
       61 ttcaatgtca aaagcaacgc cttaatttgt tcaacattca accgttcagt gttatgtgaa
      121 gtatagtcat caagtttatt gatatctatc tgacctttaa taatatattt atcataattt
      181 aagtctcgat tatccgctgg aatgcgataa tatcgtccca tatcttcagc ttttaccatt
      241 tcttctcgag aaaccagcga ctcataaagt ttttcaccgt gacgtgtccc tattatttta
      301 gtttctatgt tactgtcaca taactgtttt atggcctctg ttaagtcccc aatggtacaa
      361 gcaggtgcct tttgaacaaa aatatcgcct tgtcttccat ttttaaatgc atgaagaaca
      421 agatcaacag aatcttcaag ggacatgagg aaccttgtca tattaggatc agttacagtc
      481 aatggtttat tgagttttat tttttcaaaa aacaatggta ttactgagcc acgggatgcc
      541 ataacattcc cataacgggt tgcacacatg attgttttgt cgttatcctg ccgtctagct
      601 tttgcaatta gcaccttttc cgccatcgct tttgatattc ccatcgcatt tataggatat
      661 acggctttat ctgtactaag taaaacaaca cgttttacgc cacaatcaat agctgcattt
      721 aatacatttt cagtcccatt aacgttagtt ttaacagctt ccatcgggta aaattcacaa
      781 gatggaactt gttttaatgc tgcagcatgg aatacataat caaccccatt cattgcgcgg
      841 tatactgatt cataatcacg agtatcacca ataataaatt tcagttttgg gtttgcgaga
      901 gcaatccgca tatcttcttg ttttttctca tctcgactaa aaacaataat tcgttttaca
      961 tctgtattaa gaaagcgctt cagcacagta ttcccaaatg atccagtacc accagta
//
LOCUS       DAZKIV010000381         1006 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZKIV010000381 DAZKIV010000000
VERSION     DAZKIV010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.384"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..1006
                     /locus_tag="ACHOT6_005044"
                     /pseudo
     CDS             1..1006
                     /locus_tag="ACHOT6_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
BASE COUNT          330 a          243 c          256 g          177 t
ORIGIN      
        1 aaatcagagc gcaactgata ctctctcgtc gccagtacca gaaactgatg cagtggaaac
       61 ggcaattccg gacaacgaaa aaaaccgaat gcaaagtgga agtcgaacca tctgtagagc
      121 gtgaggggcc gttctacttc ctcttcaccg acaaggatgg cgaaaaatac ggtcgcgcaa
      181 acaaactttc tggtctggat aaggcactgg ctgccggggc tactgaaatc acgaaagaag
      241 aatatttcgc ccgcaaaaac ggtacatact caggttcaca acaaaatact ggtgcatctg
      301 acacgaccgc acaaccaggg ccggtaaaag ttaccgctga cgaagtaaac aaaattatgc
      361 aggcagccaa tatcagccag cctgacgccg ataagttgct tgctgcctct cgcggagaat
      421 ttgttgcagg gattagcgac ccgaatgatc cgaaatgggt taaggggatc cagacccgcg
      481 attctgtaaa ccagaaccag catgaatcgg aacggaacta ccaaaaagcg gaacaaaaca
      541 gcccaaatgc gttacaaaac gagccagaaa cgaaacagcc tgaaccagtg gcgcaacagg
      601 aagtggaaaa agtctgcacc gcctgcggtc agaccggcgg cggcaactgc cctgattgtg
      661 gcgcggtgat gggcgacgca acataccagg aaacattcga tgaagagtat caggttgaag
      721 ttcaggaaga tgatccggag gaaatggaag gcgctgaaca tccacacaag gagaacactg
      781 gcggcaatca gcatcacgat agcgataatg aaactggcga gacggcagat ctctcaatta
      841 aggtgaacgg tcatcaagaa atcacatcca ccagcaggac gtgtaaccat ctaatgatcg
      901 accttgaaac catgggaaaa aatcctgatg ccccgatcat ctcgataggt gcaatatttt
      961 tcgatccgca aaccggagat atgggaccgg aatttagtaa gactat
//
LOCUS       DAZKIV010000382          978 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZKIV010000382 DAZKIV010000000
VERSION     DAZKIV010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 978)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..978
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.385"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            128..760
                     /locus_tag="ACHOT6_005045"
     CDS             128..760
                     /locus_tag="ACHOT6_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9950060.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMAGKINELFIRSHSVQH"
BASE COUNT          331 a          168 c          180 g          299 t
ORIGIN      
        1 aatcagaatt atagcaaata cagattaatc gcaatttttc ccagcaaaat tacaaaatca
       61 tggtgtattg acatcatcaa tcaccaatga aattatgcaa ttatatacgg atagggaggt
      121 tcttaacatg gggctttgta gtcgttataa aagtcttaca tgcaatagtt gctcaatgca
      181 ttgccaaata atgccagaag agtcaccgcg tttgcagtac tgcgcaaact cgtgtttttg
      241 tatgtggccc gaagaaagtt catattttaa tcgtggtgtg gtagaaggta tcttaacaaa
      301 aaaccacaat gcgagattaa gcggatatat ttttgtcgat ttttcagtaa gttttttacg
      361 tctattcctt gaaaaagact ggattgacta tcttgcaagt actgacatgg ggattgtttt
      421 agtcagcgac cgcaatatgc aatcgttagc taattactgg cgaaaacaca actcagcaat
      481 atctgctgtc atatataatg atgatggtct tgatgttgcc aatgaaaaga tcagacaact
      541 atttattggt cgttatttat catttaccag aggtaacaca ttaactcaga tggaattcac
      601 cataatgggg tatatggttt ctggttataa tccatatcag attgctgaag ttctggatat
      661 ggatatccgt agcatctacg cgtacaagca acgaatcgaa aagagaatgg ctggtaaaat
      721 aaacgaatta tttattcgtt cacattcggt ccaacattga tactcaacaa tctgcaagaa
      781 tcatactctg tttatacggg catttatgga aatgcccgta ttttataaat aggccgtccc
      841 ctcaagagta aacacatagt atttaaaacc atcacacgaa aaattcagaa gcattacgaa
      901 atatgaactt acaaacaata gtggcataaa tgttaaccat gttaatttac ataaagtttt
      961 acgttgcgac attaaagc
//
LOCUS       DAZKIV010000383          967 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZKIV010000383 DAZKIV010000000
VERSION     DAZKIV010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.386"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..369)
                     /locus_tag="ACHOT6_005046"
     CDS             complement(<1..369)
                     /locus_tag="ACHOT6_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFP9950061.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMLS
                     FFGQYKTLIEDLIEHFRYGNG"
     gene            550..702
                     /locus_tag="ACHOT6_005047"
                     /pseudo
     CDS             550..702
                     /locus_tag="ACHOT6_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(727..966)
                     /locus_tag="ACHOT6_005048"
     CDS             complement(727..966)
                     /locus_tag="ACHOT6_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950062.1"
                     /translation="MNNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSIISAAIEG
                     VRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          305 a          171 c          180 g          311 t
ORIGIN      
        1 gccatttcca tatctaaaat gttcaatcag atcttcgata agtgttttat attgtccaaa
       61 aaaggaaagc atttttgcta gttctttcat ttctgcaaat aaaatatcag tgcattcacg
      121 ttgtgatatt tttgttccgg aacttgaggc actaaaagca acattaatag aacctgggcc
      181 agggaaatgg tatttaataa gtctgtaagg atcaactttt gctgagatat cgcttaggcc
      241 gagtgagaga aagtcttgtt ctaccatatc accatattgc atatcatcag cagaaaaatc
      301 atcgaataaa ctaatagttt cgaaaagtgt acagggaata ttaattggcg gaagtaatat
      361 gttattcatt tgcatttgcc ttggttatat ttaagctcaa tatcaattcg agaacaaatg
      421 ctagggtaaa aaaatattta aaaaagaagt aaacttcaca ttttttcatt acatctctga
      481 agagaacctc gttaatgatt ggtcttgctc attgttaata acaaaatgtt gaatttaccg
      541 attaggaaac tttttcctac gcggattgcc tccagctatg gcatgaactt tgagtttatg
      601 ccagaactga agtggggctt cggctaccct ggcgcgatta tctttatgat cctcgcgggc
      661 ctgacaccgt atctgtactt taagcgtaag aactggctgt aactatacga tgtcgctgtg
      721 agtaagttag tgtgctgaca gcgacatttt agccgaggga atcgcagata acataattgt
      781 tgtgaataat atccatgcca cgttgagttg gacgcttaat gactcacatc tcaccccttc
      841 aattgctgca gagataatag aaaaaagtgt caagcccaat gaaatcaata caaagtagat
      901 tgccttacat ccagtatctc ttattctttt tgctccaata ttgatattta catatgtatt
      961 attaata
//
LOCUS       DAZKIV010000384          962 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZKIV010000384 DAZKIV010000000
VERSION     DAZKIV010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.387"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..812
                     /gene="paa"
                     /locus_tag="ACHOT6_005049"
     CDS             54..812
                     /gene="paa"
                     /locus_tag="ACHOT6_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcfC family adhesin Paa"
                     /protein_id="HFP9950063.1"
                     /translation="MRNIMAGFLIFLSSAAYADINLYGPGGPHTALLEAAKLYAEKTG
                     VIVNVHYGPQNKWNEDAKKNADILFGASEQSALAIIRDHKDSFSEKDIQPLYLRKSIL
                     LVKKGNPKNIRSIDDLTRPGIGVIVNDGGGTSNTSGTGVWEDIAGRKGNIETVAAIRK
                     NIILYAPNSGTARKALENQPGADVWITWADWAASNPEIGDVVEIAPDYVIWRDMNITV
                     RQDANDETRRFAEWLQTDEAAPAFKKYGWTRKGT"
BASE COUNT          293 a          191 c          232 g          246 t
ORIGIN      
        1 tatttgtagt gtttataact ttatttcatg ataaccaata aaaggagttt tttatgagga
       61 acataatggc aggtttttta atattcctgt cttctgctgc ttatgctgat atcaatctgt
      121 atggtcctgg tggcccgcat acagccttgc ttgaagcagc caaactttat gccgaaaaaa
      181 caggtgttat agtgaacgtg cattacggtc cacagaacaa atggaatgaa gatgccaaaa
      241 aaaatgcaga tatcttgttt ggcgcatcag aacaatctgc tctggctatc attcgggacc
      301 ataaagacag cttcagtgaa aaagatattc agcctcttta tctgcgaaaa agtattttac
      361 tggtaaagaa aggtaatcct aaaaatatcc ggagtattga cgacctgacc agacccggga
      421 ttggcgtaat tgttaatgat ggtggtggta ccagtaatac atcaggcact ggcgtctggg
      481 aagatattgc cggacgtaaa gggaatatag aaactgtcgc cgcaatccga aaaaatatta
      541 ttttatatgc gcccaatagc ggaactgcac gtaaggctct tgagaatcag cctggagcag
      601 atgtctggat aacctgggct gactgggcag ccagtaatcc agaaattggt gatgtcgtgg
      661 aaatagcgcc agactacgtg atatggcgtg atatgaacat tacagtacgt caggatgcaa
      721 atgatgaaac ccgtcgattt gcagaatggc tacaaaccga tgaagcggcg cctgcattca
      781 aaaaatatgg ctggaccagg aaaggcactt gacatcctcg tccttcagga cgtggattct
      841 ttttccggat gccgcgccag cggcatgtag gggcagctca caaaacggaa aaaattgtac
      901 gctaagcctc gccaggtgaa ctgaattcat tccgatatgg gaattcccat atcgggcgaa
      961 aa
//
LOCUS       DAZKIV010000385          961 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZKIV010000385 DAZKIV010000000
VERSION     DAZKIV010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.388"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..921
                     /locus_tag="ACHOT6_005050"
     CDS             <1..921
                     /locus_tag="ACHOT6_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HFP9950064.1"
                     /translation="QGVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIP
                     VPVQEPGSGKTRTARLWVYVRDDRNAGSQMPPAVWFAYSPDRKGVHPQNHLAGYSGVL
                     QADAYGGYRVLYESGRITEAACMAHARRKIHDVHARMPTDITTEALQRIGELYAIEAE
                     VRGCTAEQRLAARKARAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWDGLNVY
                     CSNGWVEIDNNIAENALRGVAVGRKNWLFAGSDSGGEHAVVLYSLIGTCRLNNVEPEK
                     WLRYVIEHIQDWPANRVRDLLPWKVDLTSQ"
BASE COUNT          225 a          253 c          296 g          187 t
ORIGIN      
        1 cagggcgtgg agctgagccg cgccacgctg gggcgctgga caggtgccgt tgctgaactg
       61 ctggagccgc tgtatgacgt cctgcgccag tatgtgctga tgcccggtaa agtccatgcc
      121 gatgatatcc ccgtcccggt ccaggagccg ggcagtggta aaacaaggac tgcccggctg
      181 tgggtctacg tccgtgatga ccgtaacgcc ggttcacaga tgcctccggc ggtctggttc
      241 gcgtacagcc cggaccggaa aggcgtccat ccacagaatc atctggccgg ttacagcggt
      301 gtgcttcagg ccgatgctta cggtggttac cgggtgttat acgaatccgg cagaataacg
      361 gaagccgcgt gtatggctca tgcccggaga aaaatccacg atgtgcatgc aagaatgccc
      421 accgacatca ccacggaagc cctgcagcgt atcggtgaac tgtatgccat agaggcagaa
      481 gtccggggat gtacagcaga acagcgtctg gcggcaagaa aagccagagc tgcgccactg
      541 atgcagtcac tgtatgactg gatacagact cagatgaaaa cactgtcgcg tcactcggat
      601 acggcaaaag cgttcgcata cctgctgaaa cagtgggatg gcctgaacgt gtactgcagt
      661 aatggctggg tggaaatcga caacaacatc gcagagaacg ccttacgggg agtggccgta
      721 ggccggaaaa actggctctt cgcgggttcc gacagcggtg gcgaacatgc ggtggtgttg
      781 tactcgctga tcggcacatg ccgtctgaac aatgtggaac cagaaaaatg gctgcgttac
      841 gtcattgagc atatccagga ctggccggca aaccgggtac gcgatctgtt gccctggaaa
      901 gttgatctga cctctcagta aatatcaata cggttctggc gagccgctta cattcatgca
      961 g
//
LOCUS       DAZKIV010000386          945 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZKIV010000386 DAZKIV010000000
VERSION     DAZKIV010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.389"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..498
                     /locus_tag="ACHOT6_005051"
                     /pseudo
     CDS             1..498
                     /locus_tag="ACHOT6_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    127..243
                     /locus_tag="ACHOT6_005051"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          239 a          186 c          252 g          268 t
ORIGIN      
        1 tcagcatgag cgggataccg ggagtggtga tggtggactc accaccgctg aacgtcagcg
       61 tctgaaagag ctggaacgtg aaaatcgtga actgcgccgc agtaacgata tccttcgcca
      121 ggcttccgct tattttgcga aggcggagtt cgaccgcctc tggaaaaaat aatgccgctg
      181 ctggataagc tgcgtgagca gtacggggtc ggaccggtat gcagtgaact gcatattgcc
      241 ccgtcaacgt attaccactg tcagcaacag cgacatcatc ctgataaacg cagtgcccgt
      301 gcgcagcgcg atgactggct gaagagagag atacagcgcg tatacgatga aaatcatcag
      361 gtatacggtg tgcgtaaagt ctggcgccag ttgttacgcg aaggtatcag ggtggccaga
      421 tgtacagtgg cgcgcctcat ggcggttatg ggacttgccg gtgttctccg gggtaaaaag
      481 gtccgtacta ccgtcagccg atttacgggc cgtaagtaac gaagttggat gcaaatgtca
      541 gatcgtgtgc gcctgttatg gctcggctgg taagagagcc ttataggcgc atttgaaaaa
      601 actccagcta taccggaaga tatttattat taacaggaga ggtttgcttt tctgtgtagg
      661 tttagatttt gtgtttgcag ggtaaacaaa actgattaac tcgtcatttc ggtaactgaa
      721 atagtttgct gtattctgtt tacaatctct ttaataaggc atttgtcacg tggctattca
      781 tatgaaaatc atacgttatc ttacaaatca atcatctgag tgttataata taacttagct
      841 gtgatatgtg taagaatgtt taggcaatat attattatcg ctaatgttgt tttggttggg
      901 agagattctc cttgcggtag ctttttgttt ttagatgctt cttgc
//
LOCUS       DAZKIV010000387          937 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZKIV010000387 DAZKIV010000000
VERSION     DAZKIV010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..937
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.390"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(209..880)
                     /gene="queE"
                     /locus_tag="ACHOT6_005052"
     CDS             complement(209..880)
                     /gene="queE"
                     /locus_tag="ACHOT6_005052"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFP9950065.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          252 a          236 c          202 g          247 t
ORIGIN      
        1 taaaataact ctatacaaat taaaatctac caaactttac cgcaataatt ttcactccag
       61 cgaaaaatta atgccacaga atttgtagag aatgctaatg attagccctg gatgggtaaa
      121 atggaggagt tttcagaggc gacaaacaat ataaatgagt agaagtcttt acaggttcca
      181 cccatttata acgcttataa atgtttaatc aggcaatatt tagatattta tgtgtttgca
      241 tcgacaaacg ccaattacgc gcaatgcagg tttcaatgca caaacgtgtg gcatcatcct
      301 tttggctaat cggctgcagt gcaatgactc gcggtttatc atcggtcagc gttgccaata
      361 gttcatccag tgcttcaata tcgcgtacgc gccccactgg atgcttgatt tcgttggctc
      421 gctccagtgc ctgtgacaac acgtcatagc cgccgcgcat gttcagcttt ggcgatacgg
      481 taacccaggt attcggtgtg cagcgtactt catgagttcc actggtttcg atctggcagc
      541 taaaaccgtt cttttcgagc aggtcagtca gtggcagcaa atcatgaatg caaggctcac
      601 cacccgtaat caccacatgc cgcgcggtat atccctgccg accaatgaca gccagcaaat
      661 cttcactgct cgccgccccc cacttatcac tctctttggt cttcgccaga atgctgaaaa
      721 gggagacttc ccgatcctca agcttttccc aggtgtgttt ggtgtcgcac caggcacagc
      781 caaccgggca tccctgtaaa cgaataaaaa tggcggggac gccggtaaag taaccctcac
      841 cttgcagggt ctggaacatc tcgttaatcg ggtactgcat agcattctct gtgaagtgga
      901 taattgttaa ttattgcaga tcctgccgca acaatca
//
LOCUS       DAZKIV010000388          936 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZKIV010000388 DAZKIV010000000
VERSION     DAZKIV010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..936
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.391"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..607
                     /gene="nleB"
                     /locus_tag="ACHOT6_005053"
     CDS             <1..607
                     /gene="nleB"
                     /locus_tag="ACHOT6_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFP9950066.1"
                     /translation="KKLSDIYLENIHKQESYPASERDNYLLGLLREELKNIPEGKDSL
                     IESYAEKREHTWFDFFRNLAILKAGSLFTETGKTGCHNISPCSGCIYLDADMIITDKL
                     GVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKSKVDAHPYYDGLG
                     KGIKRHFNYSSLHNYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            656..>936
                     /locus_tag="ACHOT6_005054"
     CDS             656..>936
                     /locus_tag="ACHOT6_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NleE/OspZ family T3SS effector cysteine
                     methyltransferase"
                     /protein_id="HFP9950067.1"
                     /translation="MINPVTNTQGVSPINTKHAEHVVKNIYPKIKHDYFNESPNIYDK
                     KYISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENNDFLYIVVV"
BASE COUNT          310 a          141 c          183 g          302 t
ORIGIN      
        1 taaaaaacta tctgatattt accttgaaaa tatccataaa caagaatcat accctgccag
       61 tgagagggat aattatctgt taggcttatt aagagaagag ttaaaaaata tcccagaagg
      121 taaggactct ttgattgagt catatgcaga aaaaagagaa catacttggt ttgatttttt
      181 caggaatttg gccatattga aggctggaag tttgtttaca gagacgggaa aaactggatg
      241 ccataacata tcgccctgta gcggatgtat atatcttgat gccgacatga ttattaccga
      301 taaattagga gtcctgtatg ctcctgatgg tatcgctgtg catgtagatt gtaatgatga
      361 gataaaaagt cttgaaaatg gtgcgatagt tgtcaatcgt agtaatcatc cagcattact
      421 tgcaggcctc gatattatga agagtaaagt tgacgctcat ccatattatg atggtctagg
      481 aaagggtatc aagcggcatt ttaactattc atcgttacac aattataatg ctttttgtga
      541 ttttattgaa tttaagcatg aaaatattat accgaatacc agtatgtata ccagcagttc
      601 atggtaagtc ttccggttgt ggttgaaaaa tatcgttctt taggtataga ttcagatgat
      661 taatcctgtt actaatactc agggcgtgtc ccctataaat actaaacatg ctgaacatgt
      721 ggtgaaaaat atttacccga aaattaaaca tgattacttt aatgaatcac ccaatatata
      781 tgataagaag tatatatccg gaaaacgccc aatggggcag ttttctgtag atagtttata
      841 caatcctgat ttacatgcat tatgtgagct tccggacatt tgttgtaaga tcttccctaa
      901 agaaaataat gattttttat acatagttgt tgtgta
//
LOCUS       DAZKIV010000389          912 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZKIV010000389 DAZKIV010000000
VERSION     DAZKIV010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.392"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(188..730)
                     /gene="fimA"
                     /locus_tag="ACHOT6_005055"
     CDS             complement(188..730)
                     /gene="fimA"
                     /locus_tag="ACHOT6_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFP9950068.1"
                     /translation="MKLRFISSALAAALFAATGIYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT          288 a          186 c          172 g          266 t
ORIGIN      
        1 aaataagaaa tataaagaaa tgattgcttt ggtttcttac aacacatcat tcccttttca
       61 tttaataaag cggcatgata caaagtgaac atgatttcga tttacattgc caccataccg
      121 cttcagacaa ccggtacatt actttgttat aagacgtgat atataattca aaacaacgtg
      181 gttttgatta ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc cctgctgatg
      241 cacttgtacc ggtgccttta taacgtgcag aaaaatgaag aacgttggtc ccggggatca
      301 agttctggtt ggttgagaag ctattcccat caggtttgag aattgcggat gtattatcaa
      361 gtatttcaat accgacaccc gttgctgttg ttgtattggt actggatgca atggccaata
      421 aattatcatt gattgcatca gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa
      481 caggatcgca gtcgttcaac tgaatggtga atggaattaa tgcagaggta ttaccaacag
      541 cattgaaaat atcggtacga tattgaccga gtgtgacaac ctggtctgcc gagtcagtat
      601 tcactgaaca ggcagcattc accagttcgc cttcaaagtg aattgtaccg ccatctacaa
      661 cggcagcata aatacccgta gcggcgaata gtgcggcagc cagcgcagac gaaataaatc
      721 ttaatttcat atatattcct tcaatctcat ttatcgactc cacatccgta tataaccgat
      781 tactttattt aagacactga tagtagtaaa attccttttt atcctctaag aatgtcttaa
      841 ttgaaaatat gcactctatt ctaaaaaata gagagccccg ttagatgaat acttccgcgc
      901 aaaatatatt ca
//
LOCUS       DAZKIV010000390          908 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZKIV010000390 DAZKIV010000000
VERSION     DAZKIV010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.393"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(78..188)
                     /locus_tag="ACHOT6_005056"
                     /pseudo
     CDS             complement(78..188)
                     /locus_tag="ACHOT6_005056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head protein"
     gene            complement(252..>908)
                     /locus_tag="ACHOT6_005057"
     CDS             complement(252..>908)
                     /locus_tag="ACHOT6_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9950069.1"
                     /translation="GRRVSLDDILQRADVVTVGIDGGGLDDLLGMYVIGRDRETREWL
                     GWGHAWVHETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLD
                     HIGIDPSGVGQILDSLAEAGIPDESVVGISQGWKLGGAIKTTERKLAEGVLVHGGQPL
                     MAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI
                     "
BASE COUNT          201 a          302 c          204 g          201 t
ORIGIN      
        1 tttcttgttc attaagtcac ctgttttgtg ttgtggtgag aatatcacct ttcatcaggt
       61 ggccaaattt agtgtgccac tacaccatcc cgatccggag aaggcgttga cgcaatcccg
      121 gtgatcaccc gttcatcctc actgaaggat ttcaccgtca gcagggaaca ggcccgttta
      181 agagtcacat cagcctcctg aaaataaaaa aaccgccgca gcggttcatg atggttacag
      241 ggtgagcagg gttatatgaa aaaaacctca tacgctttct ttttcggttc cggattcagg
      301 gacatcaggg acaccgcatt gaagagcgcc atcagagggt caatttttcc ccgtccgctg
      361 gcctgtttgg tgataagaat ggcgttacct ttaggctcca cccgggcatt gccaacgcac
      421 caggccatca gtggctgacc accatgcacc agcactccct cagccagttt gcgctcggtg
      481 gttttgatgg ccccgcccag cttccagccc tggcttatcc ccaccacact ctcatcgggg
      541 atcccggctt ccgccagtga atccagaatc tgccccacac ctgacgggtc aataccgata
      601 tgatccagta actcagcctc atgaatacga cgcacatact ccgccacttc cgccgtgtca
      661 tccccgaccc gacggacaat cgtcatgtct ccacaggcca caaaatcctg aaaccgggat
      721 gcctcactct tccgtctgac caccgcggtt tcatgcaccc aggcatggcc ccagcccagc
      781 cattcgcggg tttccctgtc acggccaatc acgtacattc ccagcagatc atccaggccc
      841 ccgccgtcaa tccccaccgt caccacatca gcgcgctgca ggatatcgtc caggctgacg
      901 cgcctgcc
//
LOCUS       DAZKIV010000391          903 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZKIV010000391 DAZKIV010000000
VERSION     DAZKIV010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..903
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.394"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..424)
                     /gene="escT"
                     /locus_tag="ACHOT6_005058"
     CDS             complement(<1..424)
                     /gene="escT"
                     /locus_tag="ACHOT6_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscT"
                     /protein_id="HFP9950070.1"
                     /translation="MNEIMTVIVSSFYCILRPLGMFIILPIFSTGVLLSNFIRNSIMI
                     AFTLPIIVENYTFSEKLPSGIFQLTGIALKEISIGFFIGLSFTILFWAIDAAGQIIDT
                     LRGSTISSIFNPSISDSSSITGVILYQFISVIFVIHGGI"
     gene            complement(424..693)
                     /gene="escS"
                     /locus_tag="ACHOT6_005059"
     CDS             complement(424..693)
                     /gene="escS"
                     /locus_tag="ACHOT6_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscS"
                     /protein_id="HFP9950071.1"
                     /translation="MDTGYFVQLCVQTFWIIFILSLPTVIAASVIGIIISLVQAITQL
                     QDQTLPFLLKIIAVFATLALTYHWMGTTIINFSSIIFEMIPKVNG"
     gene            complement(693..>903)
                     /gene="escR"
                     /locus_tag="ACHOT6_005060"
     CDS             complement(693..>903)
                     /gene="escR"
                     /locus_tag="ACHOT6_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     protein EscR"
                     /protein_id="HFP9950072.1"
                     /translation="TMGQLEAAFKIGFLLYLPFIAIDLIISNILLALGMMMVSPVTIS
                     IPFKILLFILVGGWQKLFEFLLVVN"
BASE COUNT          334 a          175 c          156 g          238 t
ORIGIN      
        1 gtatcccacc atgaataaca aagatcacag agataaattg gtacaaaata acgccagtga
       61 tagaagatga atcacttatg gacgggttaa aaattgaaga tattgttgaa cctcttagag
      121 tatcaataat ctgcccagcc gcatctattg cccaaaaaag aatagtaaat gataacccaa
      181 tgaaaaaacc aatacttatt tcttttagcg ctattcctgt caactgaaag attccagatg
      241 gcaatttttc cgaaaatgta tagttctcta ctatgatcgg aagcgtaaac gcaatcataa
      301 ttgagttgcg aataaagtta ctgagtaaaa cacctgttga aaatattggt aggataataa
      361 acatccccag cggcctgagt atacaataaa atgatgatac tatgaccgtc attatctcat
      421 tcattagccg ttcaccttcg gaatcatttc aaatattatc gacgagaagt tgatgattgt
      481 tgttcccatc cagtgataag tcagggcaag cgtagcaaac actgctatta tttttagcaa
      541 aaaaggcaat gtttgatcct gtaactgcgt tatagcctgg accagactaa taataatacc
      601 gataacagag gccgctatga ctgtaggcaa tgagaggata aatattatcc agaacgtttg
      661 cacacataat tgaacaaaat atccagtatc catcaattca ccaccaacag aaattcgaat
      721 aatttttgcc agcctccaac aagaatgaat aacaagatct taaaaggaat cgaaattgtt
      781 actggcgata ccatcatcat acccaaggcc aataagatat tggaaatgat caaatctatc
      841 gcaataaagg gtaaatagag caaaaaacct atcttgaatg cagcttcaag ctgccccatc
      901 gtg
//
LOCUS       DAZKIV010000392          902 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZKIV010000392 DAZKIV010000000
VERSION     DAZKIV010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.395"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..902
                     /locus_tag="ACHOT6_005061"
                     /pseudo
     CDS             16..902
                     /locus_tag="ACHOT6_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          231 a          239 c          250 g          182 t
ORIGIN      
        1 gccacctgat gaaaggtgat attctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gaaccttcac ccctgagttc aggctggaat gtgcacagct gattgttgat
      121 aagggctact catatcgaca ggccagtgaa gcgatgaatg tcggttcaac cacgcttgag
      181 agctgggtac gccagctcag gcgagagcgc cagggtatta cgccctctgc cacacccatt
      241 actccagacc agcaacgtat ccgcgagctg gaaaagcaag ttcgccgtct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttaatgtccg actcgctgaa cggttcacga
      361 tagccgccag actaagtgac agccacacgg ttgtcagcct gtgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcacgagtca
      481 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtcg gggctctgcg ggcgcgcgca
      541 ctctggctga aatgctgact caaaacggcg tcccgatgag ccgttaccgt gccggacgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtat aaaaatgccc
      661 gtcaggagca taccagcctg ccgaatctgc ttgagcgtca gttcgcagta ccggagccag
      721 accgggtatg gtgtggagat attacgtatc tctgggcagg aaatcgctgg tgctatctgg
      781 cggttgttat ggatcttttt gcccgcaggg ttatcggctg gagtctgtca gcgcatgccg
      841 ataccgcact gataagcagt gccctgcgga tggcctatga gacgcgtggc caaccccggg
      901 at
//
LOCUS       DAZKIV010000393          881 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZKIV010000393 DAZKIV010000000
VERSION     DAZKIV010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.396"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            40..825
                     /locus_tag="ACHOT6_005062"
                     /pseudo
     CDS             40..825
                     /locus_tag="ACHOT6_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          288 a          180 c          190 g          223 t
ORIGIN      
        1 atattctaat cattatgaca ggcgagggag tgtccaatta tgaattcaaa aaagctttgt
       61 tgcatatgtg tgttattctc gctgcttgca ggatgtgcct ctgaatcttc tattgatgaa
      121 aagaagaaaa aagctcaagt cacacaaagt aatattaata aaaacactcc ccagcaactg
      181 acagacaaag atttattcgg taatgaaacc actctggccg tatccgaaga ggatattcaa
      241 gctgcgcttg atggagatga gttccgcgtt ccccttaatt ctcctgtaat acttgttcaa
      301 tccggcaacc gcgcaccgga aaccattatg taggaggaga tgcgaaaata ttatactgtt
      361 tccacattct ctggtatccc ggacaggcaa aagcctctga cttgtaacaa aaacaaagat
      421 aaaaatgaga acgaggatgt tgccagtgct gagaatatga actggatgca ggcactgcgt
      481 tttgtggctg caaaaggaca tcagaaagcg attattgttt accaggatac attgcagaca
      541 ggaaaatatg actctgcgct gaaatcaaca gtatggtccg actataaaaa tgaaaaactc
      601 accgacgcta tctccctgcg ctacctggta cgtttcacgc tggtggatgt ggcaacaggt
      661 gagtgggcta cctggtcgcc ggtgaattat gaatataaag tgcttccacc attgcccgac
      721 aagaatgaag ccagtacgac tgatatgaca gagcagcaaa tcatgcaact taagcaaaag
      781 acctataaag cgatggtaaa agatttggtt aaccgctatc aataataaat tatatctgcc
      841 gccaggaatt tctggcggca ataatacaaa attctttgca t
//
LOCUS       DAZKIV010000394          875 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZKIV010000394 DAZKIV010000000
VERSION     DAZKIV010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..875
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.397"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..801
                     /locus_tag="ACHOT6_005063"
     CDS             <1..801
                     /locus_tag="ACHOT6_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFP9950073.1"
                     /translation="LISAFAIGSYGLGSLGFKFIDTQLLETVGLEKTFVIWGAIALVM
                     IVFGTTLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWMLAVMFLTACMSGLYVIGV
                     AKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDKIARIRVITIGQVISLVGM
                     AALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFGLNNLAKNYGVIYLGFGIG
                     SIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQKMLREAHI"
BASE COUNT          195 a          188 c          234 g          258 t
ORIGIN      
        1 ctgatatccg cgttcgctat cggttcttat ggtctgggta gcctgggttt caaatttatc
       61 gacacgcagc tgctggaaac ggtgggtctg gaaaaaacct ttgtgatttg gggagcgatt
      121 gcgctggtga tgattgtttt cggcacaacg ttaatgaaag acgcaccaaa acaggaagtg
      181 aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat gcgtaaaccg
      241 cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct gtacgtgatt
      301 ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc cgcagccaat
      361 gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg gatactgtct
      421 gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct ggtgggtatg
      481 gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat tgcctgcgtg
      541 gcatttaact ttggcggcac tattaccgtc tttccgtcac tggtcagtga gttcttcggc
      601 ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gcttcggtat cggtagcatt
      661 tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta cgtgattttc
      721 gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga gcagaaaatg
      781 ttgcgtgagg cgcatattta gaaatatgtt gctactgaaa taaatacgag gagggagact
      841 tcaaagtctc ccttttttat ggacgcatat taaaa
//
LOCUS       DAZKIV010000395          871 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZKIV010000395 DAZKIV010000000
VERSION     DAZKIV010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.398"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..638
                     /gene="tssI"
                     /locus_tag="ACHOT6_005064"
                     /pseudo
     CDS             1..638
                     /gene="tssI"
                     /locus_tag="ACHOT6_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
     gene            637..871
                     /locus_tag="ACHOT6_005065"
                     /pseudo
     CDS             637..871
                     /locus_tag="ACHOT6_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          232 a          225 c          264 g          150 t
ORIGIN      
        1 acccgctgaa taaccacttt gcggtaatac cggcagaccg gacatggcgg ccacagccgt
       61 tgctgaaacc gctggtggac ggcccgcaga gcgccgtcgt gacgggaccg gcaggcgagg
      121 aaatcttctg tgatgaacat ggccgcgtgc gggtgaaatt taactgggac cgctataacc
      181 cgtcaaacca ggacagttca tgctggatcc gtgtggcaca ggcgtgggca ggcaccggct
      241 ttggcaacct ggcgataccg cgagtgggtc aggaggtgat tgtggacttc ctcaacggcg
      301 acccggacca gccgattatt atggggcgta cctaccacca ggaaaaccgc acacccggca
      361 gcctgccggg gacgaagacg cagatgacca ttcgttcgaa aacctataag ggcagcgggt
      421 ttaatgaact gaagtttgac gatgcgacag ggaaagaaca ggcctacatc cacgcgcaga
      481 agaacatgaa caccgaggtg ctgaataacc gcaccactga tgtgataaac aaccatgctg
      541 aaaccattgg caacaatcag atgattgcgg ttaccaacaa tcagataaag acggtgggtg
      601 ttaaccagat agagacggtg ggcagtaacc agataatgcg accaccgggg cctgccgctg
      661 gcgctcatca gcgaagacgg caatactgtg tggagtgcag aatacgatga atggggcaac
      721 cagctgaatg aagagaaccc gtattacctg tatcagccat accgtctgcc agggcagcag
      781 tacgatgagg agtcggggct ggattataac cgtcaccggt actacgatcc gttgcagggg
      841 cggtatatca cccaggaccc gattgggctg g
//
LOCUS       DAZKIV010000396          863 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZKIV010000396 DAZKIV010000000
VERSION     DAZKIV010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.399"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            71..354
                     /locus_tag="ACHOT6_005066"
                     /pseudo
     CDS             71..354
                     /locus_tag="ACHOT6_005066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
BASE COUNT          268 a          186 c          177 g          232 t
ORIGIN      
        1 cttggatttg actctatatt ttcagacacc tgttatccct taacgttact aatacttgcg
       61 tagtgaattt atgaacaatc ctaatccatg catgacgtgt ggtgcctgtt gtgcattttc
      121 cgcgtctctt tttaccaggc tgaagcagat gatgctggtg gcatcatccc cgtaaccctg
      181 actgagcaaa tctctccgaa ccatagaagc atgcagggca ctaaccagaa aaatcctcga
      241 tttgtggcct tggacggaga accgggagtc aatgcctgct gctgcattca tcagaatcgc
      301 tcgtccacct tcagggaatt tcctgtgtcc gaagaaaatg gtgtggttaa ctaagcaaaa
      361 gtatcccttt atcggtatag caacttattt tgttaacatg tggttgcaaa atggatgaca
      421 agtgtttatt ttagccacaa gaaatgtatg caaactagaa ttgggaaatg catttataaa
      481 ataaggaata gcagatatgt ttgcagcaaa gcccgaaccc agtcaaagat gtatttctgg
      541 cttcctcact actgggccaa atgatgaaaa cgcaaataat aaaccgtcaa gtgatatggc
      601 gaacagaatc aaaccagttg taagcgattt acaaatcgat atatacaact gacagtaata
      661 ctctgaagga tggattgtgt agccaagccc acataggaac tcaaccttat cggccaggtg
      721 ctgactcgcc gctaactgtg agatcattcc tacaaatcac actttccaac aataattaca
      781 aatcattaga gcatctgcaa acacgagcag agaattatct gaggtatcgc aaagcagaag
      841 agaatattct gcgttcgacc gtt
//
LOCUS       DAZKIV010000397          860 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZKIV010000397 DAZKIV010000000
VERSION     DAZKIV010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.400"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..771
                     /locus_tag="ACHOT6_005067"
     CDS             121..771
                     /locus_tag="ACHOT6_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFP9950074.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPILGGLWDHLGAREAHHLADSLTRQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
BASE COUNT          292 a          162 c          155 g          251 t
ORIGIN      
        1 aaaaacatgc atgcaataat attctattaa aaatagtatt gttttattca catatcatga
       61 gcggtatgtt gactcttgtt accaatatag ttcacaatat ggattaaaaa ggataataat
      121 atgccagtca tattaaattt ttctaatgga agtgtattgc cggaaaatga gctggaggct
      181 ttacgacata ttgcaagaag caaccagaat gacactatta ctataggagg ccgtaatatg
      241 aggcttcatt atatccagtt tatggatggt tttagtgttg agcctattct tggtggactt
      301 tgggaccatc ttggggcaag agaggctcat catctcgcag acagcctgac aagacaactt
      361 aacggaggta acacttttct gcaggcatac agcttatatc tggagcagag gcaagctgcc
      421 ccgcttgtgc aggaaagcgt cataaaaaca ctactagatc gaataaattc gaatgcattt
      481 cccgttagtt tacaagactt ttcctgtact gaggaacatc ttaattgtcc gataacgcta
      541 catattcctg agacaggtgt ttttgtcaga aatgctcgaa attcagaaat atgtgcatta
      601 tatgatcagg aagcattgac tgaacttatc ctgcgtaacg ctctccaccc cctcagccgt
      661 gacccctttg ctccagaaat gattataagc aaagacaagt gtcattttaa tataacaaaa
      721 caatgttttt acgcattacc catatacccc cttcaacaaa acagtattta aaaataaaaa
      781 cacaatataa gaagcatatt ttcaataagt taatcaccat ataaagcaag aaaaatattg
      841 tctgaataat tgttcaggca
//
LOCUS       DAZKIV010000398          849 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZKIV010000398 DAZKIV010000000
VERSION     DAZKIV010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.401"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..496
                     /locus_tag="ACHOT6_005068"
     CDS             <1..496
                     /locus_tag="ACHOT6_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9950075.1"
                     /translation="KPGEEGQKGLIGWFESGDVRAYKIRFPNGTVDVFRGWVSSIGKA
                     VTAKEVITRTVKVTNVGKPSVAEERSEITPATAIKVTPTSGTVAKGKTTTLTVSFEPE
                     SATDKTFRAVSADPSKATISVKDMTITVNGVATGKVQIPVVSGNGQFAAVAEVTVTEA
                     GAAG"
     gene            510..>849
                     /locus_tag="ACHOT6_005069"
     CDS             510..>849
                     /locus_tag="ACHOT6_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFP9950076.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYIKFVSDAEQQETTKHD
                     VVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDN"
BASE COUNT          225 a          195 c          249 g          180 t
ORIGIN      
        1 gaaaccggga gaagaaggtc agaaagggct tataggctgg tttgaaagcg gggatgtgcg
       61 ggcctataaa atccgtttcc caaatggcac ggtggatgtg ttccgtggct gggtcagcag
      121 tatcggtaag gccgtgacgg cgaaagaagt gatcacccgc acggtgaaag tcactaacgt
      181 gggcaaacct tccgtggcgg aagaacgcag cgaaattacg ccggccactg caattaaggt
      241 gacaccgaca tccggtacgg tggcaaaagg gaaaacaacc accctgactg tttcttttga
      301 gccggaaagt gcaaccgaca agacgttcag agcggtttcc gccgatccgt cgaaagccac
      361 cattagtgtg aaagatatga caattacggt aaacggcgtg gcgacaggta aggtgcagat
      421 ccctgtggtg agcggaaatg gtcagttcgc cgcagtggct gaagtcaccg ttactgaagc
      481 gggcgctgca gggtaaacgg aggtcataca tgtttctgaa aacagaacaa tttgaatata
      541 acggtgtgtc cgtcacgctt tccgaattgt ctgcgctgca gcggtttgat tatataaagt
      601 ttgtttcaga cgcagaacaa caggagacaa cgaagcatga tgtcgtgcac attaaccagc
      661 gatatctgga aacggcatcc ctgcttgtgg cgatgtcgct atggcattcc cattccctca
      721 aaggcactct ggcctctccg gagacagaga tgcagcagat ccgccgtgaa gtgatgctgg
      781 gatggcctgc tgatgcactg aatcaggcaa cgaaccgggt gctttatctt tcaggtatgc
      841 tggataacc
//
LOCUS       DAZKIV010000399          835 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZKIV010000399 DAZKIV010000000
VERSION     DAZKIV010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.402"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..234
                     /locus_tag="ACHOT6_005070"
     CDS             <1..234
                     /locus_tag="ACHOT6_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9950077.1"
                     /translation="GCGKHDGREHVQSLTAQLRLGPADILESDENGIIPEQDRVITQV
                     VILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            365..817
                     /locus_tag="ACHOT6_005071"
                     /pseudo
     CDS             365..817
                     /locus_tag="ACHOT6_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
BASE COUNT          250 a          138 c          228 g          219 t
ORIGIN      
        1 ggttgcggaa agcatgatgg ccgtgaacac gtgcagtcgc ttacagcaca actgcgactg
       61 gggccggcag acatcctgga gtcagatgag aatggtatta tcccggagca ggacagggta
      121 atcacgcagg tggtgatact ggatgcggat aaaaagcaga tacagtgcgt ggtaagaccg
      181 ctgcaaatcc tgcgtgctga cgggacgtgg gaaaatattg gcggaatgaa atagccgaca
      241 gcttcacaaa aaccggagtc cggctccggt ttttgttgtc atgtatgggg gctgtttgtt
      301 atgacaccct gtgtttggaa tgaatattta ataacaggtg tctggaaata taggggcaaa
      361 tccactggat aggctattgg ggcgtgagaa tcgaatggag agaagggctg tggctctgga
      421 aaggcaatta aatggaggtg tcgatttttt aaggagtgtt aataactatt ttcagagtgt
      481 catggcagaa cacagagaaa ataaaacaag taataaaata ttaatggaaa aaataaattc
      541 ttgtgtattt ggaacggatt ctaatcactt ttcttgcccg gagtcatttt tgacatgccc
      601 gataacgctg gacacacctg agaatggagt gttcatgaga aactcacaag gtgctgagat
      661 atgctctcta tatgataagg acgcgttagt gcaacttgtt gaaactggtg gagctcatcc
      721 tctgagtcga gaacctataa cagaatcaat gattatgaga aaagacgaat gtcactttga
      781 ttcaaaaaaa gaagcctttg ttgcaagtga tgcttaattt tttctgttgg tgtgt
//
LOCUS       DAZKIV010000400          826 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZKIV010000400 DAZKIV010000000
VERSION     DAZKIV010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.403"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>826
                     /locus_tag="ACHOT6_005072"
     CDS             <1..>826
                     /locus_tag="ACHOT6_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9950078.1"
                     /translation="PVTRTITSANIDRLRVTFGVQSLVQTTSQGDRNPASVRLLIQLQ
                     RNGNWVTEKDVTINGKTTSQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNKTLWS
                     SYTEIIDVKQCYPNTAIVGLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSG
                     IWDGSLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYAIGQYCDQRVPDGF
                     GGTEPRMTFNAYLSQQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTN"
BASE COUNT          180 a          238 c          253 g          155 t
ORIGIN      
        1 gccggtgacg cgcaccatta cgtccgcgaa cattgaccgc ctgcgggtta ccttcggggt
       61 gcagtcactg gtgcagacca cctcacaggg tgaccgtaac ccggcatccg tccgcctgct
      121 gattcagctg cagcgtaacg gtaactgggt gacggaaaag gatgtcacca ttaacggcaa
      181 gaccacctca cagttcctcg cttcggtgat tctggataat ctgcctcccc gcccctttaa
      241 catccggatg gtcagggaga cggcggacag caccacggac cagctgcaga ataagacgct
      301 ctggtcgtca tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcgattgt
      361 ggggctgcag gtggatgcgg agcagtttgg cggtcagcag atgacggtga actaccatat
      421 ccgcggtcgc atcattcagg taccgtcaaa ctatgacccg gaaaaacgca cgtacagcgg
      481 catctgggac ggcagcctga aaccggcata cagcaacaac ccggcctggt gcctgtggga
      541 catgctgact cacccgcgct acggcatggg aaaacgcctg ggggccgcgg atgtggacaa
      601 gtgggcgctg tatgccatcg ggcagtactg tgaccagcgt gtcccggacg gcttcggagg
      661 gacagagccg cggatgacct ttaatgcgta cctgtcacaa cagcgtaagg cgtgggacgt
      721 tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacggcc agacgctgac
      781 gttcgttcag gaccgtccgt cggatgtggt gtggccgtac accaac
//
LOCUS       DAZKIV010000401          825 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZKIV010000401 DAZKIV010000000
VERSION     DAZKIV010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.404"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            182..257
                     /locus_tag="ACHOT6_005073"
     tRNA            182..257
                     /locus_tag="ACHOT6_005073"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:215..217,aa:Ile,seq:cat)
BASE COUNT          242 a          155 c          183 g          245 t
ORIGIN      
        1 taattattaa catttgcttg ttgactgcga gtatcgactt tgcgaatgga agcggtgtcg
       61 ggttttatgt cgcgtgtaat gacgagcaag gatgaatgag aacgcataaa aatctgttat
      121 ttacatcata tgatcatcat gtttattgcg tggtttataa gtattgttat tgcacactca
      181 aggcccttta gctcagtggt tagagcaggc gactcataat cgcttggtcg ctggttcaag
      241 tccagcaagg gccaccaaat cattatttac gtatcaggaa gagttaaggg tacaaggggt
      301 actcgatggc ggtctatatt tcatgactaa cgccatttca aggaagaagt gcggtgggtt
      361 gtttcgaatg agggctggtg ttttggtacg tggcacgcca ctgctatgta tttatccatc
      421 agtactcatt caaaagtacc tggaaaaaat gcccgctatg ccaagcgggc aaattgctta
      481 catgtggaat taagtaatta acgataaagc cagagtatta tcatttatta taccccttaa
      541 tttaatcagg ttaattactc aaatcactga actattctct gttcgctcac tgatgatgag
      601 tgagtaccac aaccaatgta tgtagaacaa tgccgtagag taaggatgaa atgtctctgc
      661 cacgccttaa acatctacta ctgaccagaa cacccgccgc aggcagatga gttttgtgag
      721 cgttacagga gcgtggctat gctcattaac caacgctatt gagatataaa tttctaacac
      781 tcgaaaaacg aatttcacta ataaattata acgggtggtt tttat
//
LOCUS       DAZKIV010000402          819 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZKIV010000402 DAZKIV010000000
VERSION     DAZKIV010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.405"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..706
                     /gene="ydeO"
                     /locus_tag="ACHOT6_005074"
     CDS             <1..706
                     /gene="ydeO"
                     /locus_tag="ACHOT6_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFP9950079.1"
                     /translation="DKDYAFSDGEILMVDNAVRTHFEPYERHFKEIGFNENTIKKYLQ
                     CTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERNHHNFSELLLFSCLSIFAAC
                     KGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYISESLLKKKLKQEQTTFS
                     QILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFRKHFGNSPKRVSKEYRCQ
                     RHTGMNTGNTMSALAI"
BASE COUNT          254 a          158 c          167 g          240 t
ORIGIN      
        1 agataaagat tacgccttct ctgacggcga gatcctgatg gtagataacg ctgttcgtac
       61 gcattttgaa ccttatgagc ggcattttaa agaaatcgga tttaatgaaa ataccattaa
      121 aaaatatcta caatgcacta acatccagac agtgacgatg cctgttcctg cgaagttttt
      181 acgtgcttca aatgtaccga ctggattgct taatgaaatg attgcttatc tcaactcgga
      241 agaacgcaat catcataatt tttcagaact tttgcttttt tcttgcctgt ctatttttgc
      301 cgcatgcaaa ggtttcatta cactattaac taacggtgtg ctatccgttt ccgggaaagt
      361 gagaaatatt gtcaacatga agctggcgca cccatggaag ctgaaagata tttgtgactg
      421 cctgtacatc agtgaaagcc tgttgaagaa aaaacttaag caagagcaaa cgacattctc
      481 acagattctt ttagatgcaa gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc
      541 agtcaataaa attgccgaac aatgtggtta tgccagtaca tcttatttta tttatgcgtt
      601 ccgtaaacat ttcggcaaca gtccgaagag agtttctaag gagtaccgtt gtcaaaggca
      661 cacgggtatg aatacgggca acacgatgag tgctttagct atttgattat ttgctaacga
      721 gtagtcaacc acgcacgcta cgtaaaaatt aaaatggggc agtcattccc tgccccgcgt
      781 tgtttttagg cgatatattt attgcaataa ataagtgac
//
LOCUS       DAZKIV010000403          801 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZKIV010000403 DAZKIV010000000
VERSION     DAZKIV010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..801
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.406"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..138
                     /gene="tnpB"
                     /locus_tag="ACHOT6_005075"
     CDS             <1..138
                     /gene="tnpB"
                     /locus_tag="ACHOT6_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9950080.1"
                     /translation="LERGRFAWPSARDGKVFLTQAQLAMLLEGIDWRQPKRLLTSLTM
                     L"
     gene            188..>801
                     /locus_tag="ACHOT6_005076"
     CDS             188..>801
                     /locus_tag="ACHOT6_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFP9950081.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYG"
BASE COUNT          175 a          239 c          236 g          151 t
ORIGIN      
        1 ctggagcgtg ggcgcttcgc ctggccgtca gcccgtgatg gcaaagtgtt ccttacgcag
       61 gcgcagctgg cgatgctgct ggaaggtatc gactggcgac agcctaagcg gctgctgacc
      121 tccctgacca tgctgtaaat ctctttatcc tggttgtcac agaataagcc cggtaaaata
      181 cgggcttatg aacgacatct cttctgacga catcttcctg ctgaaacagc gcctggccga
      241 acaggaagcg ctgatccacg ccctgcagga aaagctgagc aaccgggagc gcgaaataga
      301 ccatctgcag gcgcagctgg ataaactccg ccggatgaac ttcggcagtc gttccgaaaa
      361 agtctcccgc cgtatcgcac aaatggaagc cgatctgaac cggcttcaga aagagagcga
      421 tacgctgact ggtagggtgt atgacccggc agtacagcgt ccgttgcgtc agacccgcac
      481 ccgtaagccg ttccctgaat cactaccccg tgacgaaaag cgactgttgc ctgcggcgcc
      541 gtgctgcccg aactgcggcg gttcactgag ctatctgggc gaggataccg ccgaacagct
      601 ggagttgatg cgtagcgcct tccgggttat ccggacggta cgggaaaaac atgcctgtac
      661 tcagtgcgat gccatcgtgc aggcacctgc accttcgcgg cccatcgagc ggggtatcgc
      721 cggaccgggg ctgctggccc gcgtgctgac ctcgaagtat gcagagcaca ccccgctgta
      781 tcgccagtca gaaatatacg g
//
LOCUS       DAZKIV010000404          795 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZKIV010000404 DAZKIV010000000
VERSION     DAZKIV010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.407"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            39..314
                     /gene="yddL"
                     /locus_tag="ACHOT6_005077"
                     /pseudo
     CDS             39..314
                     /gene="yddL"
                     /locus_tag="ACHOT6_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            353..>795
                     /locus_tag="ACHOT6_005078"
     CDS             353..>795
                     /locus_tag="ACHOT6_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFP9950082.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLIKLSLNSNALESINFP"
BASE COUNT          287 a          156 c          134 g          218 t
ORIGIN      
        1 gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat gaaattaaaa atagttgcgg
       61 tggttgtaac tggtttgtta gctgcgaacg tagcacacgc tgccgaagtc tataacaagg
      121 atggtaataa actcgacctt tatggcaagg ttaccgctct acgttatttt actgatgata
      181 agcgtgacga tggtgataaa aattatgccc gtctcggctt taaaggagaa acgcaaatca
      241 atgatcaaat gattggtttt ggtcactggg aatatgattt taaaggctat aacgatgaag
      301 ccaacggctc gcgcggcaag aatctctgat tgagttaagt gcccatcaca atatgatcac
      361 tgaccttata ttacacaatc atcccaggat gaaaacaatc actttaaacg acaaccatat
      421 tgcacattta aacgccaaaa acactacaaa actggaatat ttaaacttaa gcaataacaa
      481 tttactgcca accaatgaca ttgatcaact aatatcatca aaacatcttt ggcatgtatt
      541 agttaacggc atcaacaatg atccacttgc acaaatgcag tactggactg cagtaagaaa
      601 tataattgat gacactaatg aagtgaccat tgatttatca ggacttaatt taaccactca
      661 accaccaggg ctgcaaaact tcacatctat caatcttgat aataaccaac tcacacattt
      721 tgatgcaacc aactacgata gactcataaa gctaagtctg aatagtaatg ctcttgagtc
      781 aataaatttt cctca
//
LOCUS       DAZKIV010000405          789 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZKIV010000405 DAZKIV010000000
VERSION     DAZKIV010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..789
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.408"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(176..370)
                     /locus_tag="ACHOT6_005079"
                     /pseudo
     CDS             complement(176..370)
                     /locus_tag="ACHOT6_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          200 a          177 c          177 g          235 t
ORIGIN      
        1 gctttttcga ttgttctccc tgctaattat tgggatggat tgagattgtt ttgtcggaac
       61 tgatacgctg gcgctaatat aaagacggcg caatggccgc cacaggttct ttctgctcat
      121 ccggcgttat ttctacaggt aataattcaa cgccagtgtc agaagaggtt gttaccggaa
      181 gacgccgcga aacacgccct tcgttctgcc agagcctgag ccatttgaaa ataacattat
      241 cattgacgcc attttctcgt gcaatctgtg caacacaagc tccaggttgt gatgccagtt
      301 caaccatacg aagtttgaat tcattcgaat actttttacg aggttctttt cgccagtcct
      361 gtaattccat acttagatgt ccgtctgtgt cagatgggcg tctaagttac caattcttgt
      421 ctaatggcta catacggcga tcggtttacg cttacggaca aaaaatcacc ttgcgctaat
      481 gctctgtctc aggtcactaa tcacctctaa ctcattgatt aatagtggtg cgaaagattg
      541 gtgagatatc gttgcgtaac acttcctggc agaagggaca gggcagactc atgatttgta
      601 attattgttg gaaagtgtaa tttgtaggaa tgatctcaca gttagcggcg agtcagcacc
      661 tggccgataa ggttgagttc ctatgtgggc ttgttaacaa acatcaccat gacatgacaa
      721 caaaaaccgg agccagactc cggtttttgt gaagctgtcg gctatttcat tccgccaata
      781 ttttcccac
//
LOCUS       DAZKIV010000406          781 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZKIV010000406 DAZKIV010000000
VERSION     DAZKIV010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.409"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            152..748
                     /gene="fimE"
                     /locus_tag="ACHOT6_005080"
     CDS             152..748
                     /gene="fimE"
                     /locus_tag="ACHOT6_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFP9950083.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          211 a          144 c          186 g          240 t
ORIGIN      
        1 tattttattt ttgtatgata tttaatgtaa ttgactgata gccacatcac tccgtgtgtg
       61 gttatctttt tatctattgg gctaattttg accgattgag gtttcctata ggtattcatt
      121 caaatatatc tcagttagga gtactactat tgtgagtaaa cgtcgttatc ttaccggtaa
      181 agaagttcag gccatgatgc aggcggtttg ttacggggca acgggagcca gagattattg
      241 tcttattctg ttggcatatc ggcatgggat gcgtattagt gaactgcttg atctgcatta
      301 tcaggacctt gaccttaatg aaggtagaat aaatattcgc cgactgaaga acggattttc
      361 taccgttcac ccgttacgtt ttgatgagcg tgaagccgtg gaacgatgga cccaggaacg
      421 tgctaactgg aaaggcgctg accggactga cgctatattt atttctcgcc gcgggagtcg
      481 gctttctcgc cagcaggcct atcgcattat tcgcgatgcc ggtattgaag ctggaaccgt
      541 aacgcagact catcctcata tgttaaggca tgcttgcggt tatgaactgg cggagcgtgg
      601 tgcagatact cgtttaattc aggattatct cgggcatcga aatattcgcc atactgtgcg
      661 ttataccgcc agtaatgctg ctcgttttgc cggattatgg gaaagaaata atctcataaa
      721 cgaaaaatta aaaagagaag aggtttgatt taacttattg ataataaagt taaaaaacaa
      781 a
//
LOCUS       DAZKIV010000407          775 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZKIV010000407 DAZKIV010000000
VERSION     DAZKIV010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.410"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(25..>775)
                     /locus_tag="ACHOT6_005081"
     rRNA            complement(25..>775)
                     /locus_tag="ACHOT6_005081"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          173 a          250 c          173 g          179 t
ORIGIN      
        1 ccgccaaaac accttcggcg ttgtaaggtt aagcctcacg gttcattagt accggttagc
       61 tcaacgcatc gctgcgctta cacacccggc ctatcaacgt cgtcgtcttc aacgttcctt
      121 caggactctc aaggagtcag ggagaactca tctcggggca agtttcgtgc ttagatgctt
      181 tcagcactta tctcttccgc atttagctac cgggcagtgc cattggcatg acaacccgaa
      241 caccagtgat gcgtccactc cggtcctctc gtactaggag cagcccccct cagttctcca
      301 gcgcccacgg cagataggga ccgaactgtc tcacgacgtt ctaaacccag ctcgcgtacc
      361 actttaaatg gcgaacagcc atacccttgg gacctacttc agccccagga tgtgatgagc
      421 cgacatcgag gtgccaaaca ccgccgtcga tatgaactct tgggcggtat cagcctgtta
      481 tccccggagt accttttatc cgttgagcga tggcccttcc attcagaacc accggatcac
      541 tatgacctgc tttcgcacct gctcgcgccg tcacgctcgc agtcaagctg gcttatgcca
      601 ttgcactaac ctcctgatgt ccgaccagga ttagccaacc ttcgtgctcc tccgttactc
      661 tttaggagga gaccgcccca gtcaaactac ccaccagaca ctgtccgcaa cccggattac
      721 gggtcaacgt tagaacatca aacattaaag ggtggtattt caaggtcggc tccat
//
LOCUS       DAZKIV010000408          767 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZKIV010000408 DAZKIV010000000
VERSION     DAZKIV010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.411"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..>767
                     /gene="sctD"
                     /locus_tag="ACHOT6_005082"
     CDS             114..>767
                     /gene="sctD"
                     /locus_tag="ACHOT6_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner membrane ring
                     subunit SctD"
                     /protein_id="HFP9950084.1"
                     /translation="MLSSYKIKLLNGAMRNRELQLPMGNLTIGTEDNDIVYFPLEQGL
                     NQFLLDIREEGVFLLSPIEFWIDGQPTPYEADQPLPIGKVIDIAGCGFIIGDIDHALP
                     LSDMPERFSARNQRKKRLILAGVIGAAIALSGAIGSYVLLSPKAELPTFTRADVYQQL
                     KENKLYAITLVWHGKNIALYGRCESTTDLTPFFNYLKEKNIFYYNSIICNNQIISAIN
                     "
BASE COUNT          232 a          164 c          138 g          233 t
ORIGIN      
        1 tattccatta aatagtactg tacaacagct tcctttctac tctattctta gtcagtgtaa
       61 gttcaccata ttttttctca ttgttcaacc accaggacga ataaaattta aaaatgttat
      121 cctcatataa aataaaactt ctcaatggtg caatgcgtaa tcgggaatta caacttccga
      181 tgggaaacct aacgataggc acagaagata acgatattgt ttactttccc cttgaacaag
      241 gcctcaatca gtttttactt gatatacgtg aggaaggtgt tttcctttta tctccaattg
      301 aattttggat tgatggacag cccactccct atgaagcaga tcaaccatta cctatcggaa
      361 aggtcatcga tatagcgggg tgtggcttca tcattggtga tattgaccac gccctgccat
      421 tgtctgatat gccagaaagg ttctctgcca ggaatcaacg caaaaagcgt cttatcctgg
      481 caggcgtgat tggtgccgct attgcgttgt ctggagccat cggcagttac gttttgcttt
      541 ccccaaaagc tgaactccca acgtttacca gagctgatgt atatcagcag ctaaaagaaa
      601 acaaacttta tgcaatcacc ctggtatggc atgggaaaaa tatcgcgctt tatgggcgtt
      661 gcgaatcaac cacggattta actccatttt ttaattacct gaaagaaaaa aatatttttt
      721 attacaatag catcatttgc aataaccaaa tcatatccgc aattaat
//
LOCUS       DAZKIV010000409          759 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZKIV010000409 DAZKIV010000000
VERSION     DAZKIV010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.412"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..651
                     /locus_tag="ACHOT6_005083"
     CDS             <1..651
                     /locus_tag="ACHOT6_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEc48 family transposase"
                     /protein_id="HFP9950085.1"
                     /translation="RRQSRNEGLPFVNAKRVYRIMSENNLLLLHDKPSRQQREHKGRI
                     SVKESDQRWCSDGFEFGCDDGEKLRVTFVLDCCAREAIDWAASTGGYDKATVQDVMLG
                     AIEKRFGDKVPEQSIQWLTDNGSAYRAHETRQFARELNLKPCTTAISSPQSNGMAERF
                     VKTMKEDYIAFMPKPNVRTALHNLAVAIEHYNENHPHSALGYRSPREYRRQRVMLT"
     gene            complement(665..759)
                     /locus_tag="ACHOT6_005084"
                     /pseudo
     CDS             complement(665..759)
                     /locus_tag="ACHOT6_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          230 a          155 c          207 g          167 t
ORIGIN      
        1 aggcggcagt cccgaaatga aggactaccg ttcgtgaatg caaagcgtgt ttaccggata
       61 atgagcgaaa ataacctgtt attgttacat gacaaaccat cacgacagca gcgggaacat
      121 aaaggccgaa tatcggtgaa ggaaagcgac cagcgttggt gttccgatgg ctttgagttc
      181 ggttgcgatg atggcgaaaa actgcgtgtt acgttcgtcc tagactgttg cgctcgtgaa
      241 gccatagact gggccgcaag cacgggaggt tacgataaag cgacagtgca ggatgtgatg
      301 ttaggagcga tagaaaaacg ctttggcgat aaagtaccgg aacagtccat ccagtggctg
      361 acagataacg gttcagcgta cagagcgcat gaaacacggc agttcgccag agagctgaat
      421 ctgaagccct gcacaacggc aatcagtagc cctcagagta atggaatggc agaacggttc
      481 gtgaaaacga tgaaggaaga ctacattgcg ttcatgccga aaccgaatgt aagaacggca
      541 ttgcataacc ttgcagtggc gatcgagcat tacaatgaaa accatccgca tagtgcgttg
      601 ggttatcgct ctccgcgaga atatcgacgt cagcgggtaa tgttaactta agatacaacc
      661 cctgtctgga aataaggggg caagaacatt gtaaaagaag atgttcctgt gctggatata
      721 attgctcatt ctgttgttga tagataataa ctttatcaa
//
LOCUS       DAZKIV010000410          754 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZKIV010000410 DAZKIV010000000
VERSION     DAZKIV010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.413"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(31..642)
                     /locus_tag="ACHOT6_005085"
     CDS             complement(31..642)
                     /locus_tag="ACHOT6_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector protein Map"
                     /protein_id="HFP9950086.1"
                     /translation="MFSPTAMVGRALAQAVTQTFRPAVTKVATQAGMGASGMRFTPMQ
                     SNLMINHGKLTTQLLQAVAKQTGSSDTQQWFRQEQITFLSRAVNKTVDDYCMSNNSAI
                     SKETKCRIFKEVESAIQQPLDMNCAQSSISHFLQSNKYFNQKVDEQCGKGVDPITRFN
                     TQTRMIEQASREIFEQNFGTVKVSDIKALTEKAIAENVQDTRL"
BASE COUNT          212 a          173 c          130 g          239 t
ORIGIN      
        1 atcaagaaaa aacaaagcag tgagtccccc ctacagtcga gtatcctgca cattttctgc
       61 aatcgccttc tcagttagag ccttgatatc actgactttc acggtgccaa aattctgttc
      121 aaagatttct cgcgaggcct gttctatcat cctggtttga gtgttaaaac gtgtaatggg
      181 atccactcct ttaccacact gctcatcaac tttctgatta aagtatttat tggattgcaa
      241 aaagtgacta attgatgatt gtgcacagtt catatcgaga ggctgctgta tcgcgctttc
      301 aacctcttta aaaatgcgac atttagtctc cttactaatt gccgaattat tactcatgca
      361 atagtcatcc acagttttgt ttactgctct ggatagaaaa gtaatctgct cttgcctgaa
      421 ccattgctgg gtatcgctac taccagtttg tttggctacg gcctgtagta gctgggtagt
      481 cagtttgcca tgattaatca taaggttcga ctgcataggt gtgaacctca taccactggc
      541 tcccatgccc gcttgcgtcg caaccttggt tacagcgggt ctaaaagttt gtgtaaccgc
      601 ctgagctaac gctctaccta ccattgccgt tggactaaac atgctataaa cctttataaa
      661 ttacataaaa acatttacac ctataaaaat aaaacaaaca aataaaacta taacattatt
      721 ttatgatttt aattttcctc cagaactttt cctc
//
LOCUS       DAZKIV010000411          742 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZKIV010000411 DAZKIV010000000
VERSION     DAZKIV010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.414"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>742
                     /locus_tag="ACHOT6_005086"
     CDS             <1..>742
                     /locus_tag="ACHOT6_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HFP9950087.1"
                     /translation="LLAFLAPVLAIILFKQSKEHKAAIDNQTANNIALSSQLSENQEK
                     LAQTARDLSELEGRAAPLWQYEELHSAVMEAENKIKNADSIARQKIEEAQIKAAKTVN
                     EASYQAQITISNANSEAIAITKDARDARLKAKERLDNANSKANELISNANDNAVKIIS
                     DAEERAKEIAGSAYEAKEFAEKYEAVAKSMKNKIEGYGDEWIIPNRSVLDELAENYEF
                     TDAGKELQKARELTKSLIKTNKAASCDYV"
BASE COUNT          300 a          135 c          154 g          153 t
ORIGIN      
        1 tcttattagc atttctcgcc cctgttctag ctataatatt atttaagcaa agtaaagaac
       61 acaaagccgc catagataac cagacggcta acaacatagc cctttccagc caactgagtg
      121 aaaatcaaga aaagttagca cagactgcac gagatctatc agagcttgaa gggcgagcag
      181 caccattatg gcaatacgaa gaattgcaca gcgcagtgat ggaggcagag aataagataa
      241 aaaatgcaga ctcaatagct aggcaaaaaa tagaagaagc ccaaataaag gcagctaaga
      301 cagtaaacga agcaagttat caagctcaga taacaataag caacgctaat agcgaagcta
      361 tagcaatcac caaagacgct cgcgatgcac gcctgaaagc caaagaacgt cttgataatg
      421 ccaacagtaa agcaaatgag ctgatctcaa atgctaatga caacgcagta aaaattattt
      481 ccgatgcaga agaaagagca aaagagattg ctggttcagc atatgaagct aaagagtttg
      541 cagaaaaata tgaagcagtt gccaaatcaa tgaaaaataa aattgaaggt tatggcgatg
      601 aatggatcat ccctaaccgt agtgtacttg atgaattggc agaaaattat gagtttacag
      661 atgcaggcaa ggaattacaa aaagccaggg agttaacaaa atcattaata aaaactaata
      721 aagcagcttc gtgtgattat gt
//
LOCUS       DAZKIV010000412          740 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZKIV010000412 DAZKIV010000000
VERSION     DAZKIV010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.415"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            143..727
                     /gene="yadN"
                     /locus_tag="ACHOT6_005087"
     CDS             143..727
                     /gene="yadN"
                     /locus_tag="ACHOT6_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFP9950088.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNGTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
BASE COUNT          213 a          151 c          153 g          223 t
ORIGIN      
        1 gtcctaatga acaatggttg taaattttgg taacaaatat cattgttcat taatatctag
       61 catctgctta taacatttgt gtcatgaata gtcattcttc aaaccagtag tctgactaat
      121 ggaattttat tcaaggattt ttatgtctaa aaaattaggt tttgccctgt ctggtcttat
      181 gctggcaatg gttgcaggta ctgcttctgc tgatatggat ggtggtcagt taaatatcag
      241 tggtctggtt gttgataaca cctgtgaaac ccgcgttgac ggcggtaata aagatggtct
      301 gatcctgctg caaactgcaa ctgttggtga aatcgatgct ggcgtgctga atggcaccgt
      361 tggcgctaaa gccaaacctt tcagcatcac cgttgactgc agcaaagcaa atccgaatcc
      421 aggtagtaca gctaaaatga ccttcggttc tgttttcttt ggtaatagca aaggaacgct
      481 gaacaacgac atgtctatta acaacccgtc tgacggcgtg aatatcgctc tgcacaatat
      541 tgatggttcc actatcaaac aggttcaaat taacaaccct ggcgatgtgt atactaaagc
      601 cctggatgca acgacaaaat ctgctgttta tgattttaaa gcgtcttacg ttcgtgccgt
      661 cgcagaccaa acagcaactg ctggttatgt aaaaactaac actgcataca ccattactta
      721 tcagtaagta ttcaccaatt
//
LOCUS       DAZKIV010000413          734 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZKIV010000413 DAZKIV010000000
VERSION     DAZKIV010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.416"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>734
                     /locus_tag="ACHOT6_005088"
     CDS             <1..>734
                     /locus_tag="ACHOT6_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFP9950089.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSSDDKDVIAIDGK
                     TLRHSYDKSRRKGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNPAHDCSAISE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYI
                     SSADLTAEKFATAIRNHWHVENKLHW"
BASE COUNT          239 a          140 c          166 g          189 t
ORIGIN      
        1 gtattcctgt tcacgatact attgccagag ttgtatcctg tatcagtcct gcaaaatttc
       61 atgagtgctt tattaactgg atgcgtgact gccattcttc agacgataaa gacgtcatcg
      121 caattgatgg aaaaacgctc cggcactctt atgacaagag tcgccgcaag ggagcgattc
      181 atgtcattag tgcgttctca acaatgcaca gtctggtcat cggacagatc aagacggatg
      241 agaaatctaa tgagattaca gctatccctg aacttcttaa catgttagat attaaaggaa
      301 aaattatcac aactgatgcg atgggttgcc agaaagatat tgcagagaag atacaaaaac
      361 agggaggtga ttatttattc gctgtaaaag gaaaccaggg gcggcttaat aaagcctttg
      421 aggaaaaatt tccgctgaaa gaattaaata atccagcgca tgactgttcc gcaattagtg
      481 aaaagagtca cggcagagaa gaaatccgtc ttcatattgt ttgcgatgtc cctgatgaac
      541 ttattgattt cacgtttgaa tggaaaggac tgaagaaatt atgcgtggca gtctcctttc
      601 ggtccataat agcagaacaa aagaaagagc cagaaatgac ggtcagatat tatatcagtt
      661 ctgctgattt aactgcagaa aagttcgcca cagcaatccg aaaccactgg cacgtggaga
      721 ataagctgca ctgg
//
LOCUS       DAZKIV010000414          718 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZKIV010000414 DAZKIV010000000
VERSION     DAZKIV010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..718
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.417"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            41..>718
                     /locus_tag="ACHOT6_005089"
     CDS             41..>718
                     /locus_tag="ACHOT6_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFP9950090.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDD"
BASE COUNT          235 a          126 c          161 g          196 t
ORIGIN      
        1 ttaccagtaa tgtaaataaa ttttcgagga gatcattcca gtgggacgta aatgggccaa
       61 tattgttgct aaaaaaacgg ctaaagacgg tgcaacgtct aaaatttatg caaaattcgg
      121 tgtagaaatc tatgctgctg ctaaacaagg tgaacccgat ccagaattaa acacatcttt
      181 aaaattcgtt attgaacgtg caaagcaggc acaagttcca aagcacgtta ttgataaagc
      241 aattgataaa gccaaaggcg gcggagatga aacgttcgtg cagggacgtt atgaaggctt
      301 tggtcctaat ggctcaatga ttatcgccga gacattgact tcaaatgtta accgtacgat
      361 tgctaacgtt cgcacaattt tcaataaaaa aggcggcaat atcggagcgg caggttctgt
      421 cagctatatg tttgacaata cgggtgtgat tgtatttaaa ggatcagacc ctgaccatat
      481 ttttgaaatt ttacttgaag ctgaagttga tgttcgtgat gtgactgaag aagaaggtaa
      541 cattgttatt tataccgaac ctactgacct tcataaagga atcgcggctc taaaagcagc
      601 tggaatcact gagttctcaa caacagaatt agaaatgatt gcgcaatctg aagttgagct
      661 ttccccagaa gatttagaaa tctttgaagg gcttgttgat gcccttgaag atgacgac
//
LOCUS       DAZKIV010000415          714 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZKIV010000415 DAZKIV010000000
VERSION     DAZKIV010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..714
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.418"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..714
                     /locus_tag="ACHOT6_005090"
                     /pseudo
     CDS             1..714
                     /locus_tag="ACHOT6_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          163 a          195 c          214 g          142 t
ORIGIN      
        1 gcaaaatcag cagacgtctt ctgaacgtga actggagatg tctaccgaga ttgcacgtct
       61 caaacgccag ctggcagaac gggatgaaga gctggctatc ctccaaaagg ccgcgacata
      121 cttcgcgaag cgcctgaaat gaagtatgtc tttattgaaa aacatcaggc tgagttcagc
      181 atcaaagcaa tgtgccgcgt gctccgggtg gcccgcagcg gctggtatac gtggtgtcag
      241 cggcggacaa ggataagcac gcgtcagcag ttccgccaac actgcgacag cgttgtcctc
      301 gcggctttta cccggtcaaa acagcgttac ggtgccccac gcctgacgga tgaactgcgt
      361 gctcagggtt acccctttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcagggactg
      421 agggcaaagg cctcccggaa gttcagcccg gtcagctacc gcgcacacgg cctgcctgtg
      481 tcagaaaatc tgttggagca ggatttttac gccagtggcc cgaaccagaa gtgggcagga
      541 gacatcacgt acttacgtac agatgaaggc tggctgtatc tggcagtggt cattgacctg
      601 tggtcacgtg ccgttattgg ctggtcaatg tcgccacgca tgacggcgca actggcctgc
      661 gatgccctgc agatggcgct gtggcggcgt aagaggcccc ggaacgttat cgtt
//
LOCUS       DAZKIV010000416          706 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZKIV010000416 DAZKIV010000000
VERSION     DAZKIV010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.419"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..638)
                     /locus_tag="ACHOT6_005091"
     CDS             complement(<1..638)
                     /locus_tag="ACHOT6_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFP9950091.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVT"
BASE COUNT          172 a          179 c          173 g          182 t
ORIGIN      
        1 taggtcacgc cgaaagtcgg caccacgccg atggtgtttt cgtcacaggc ttcaatcatg
       61 cgtttcgggt ccataaacaa ctgaccgggg cgcataggga tctcacgcag ctccacatcc
      121 cagtagcggg cgaatttatg ccagcagatt tgtaccggac cgcacaccag gtttggttta
      181 tccgttggtt tgcctgcagc ttccatacgc ttgcgccaac gccatttcat cgccatcccg
      241 ccgagcatac aggcctcgga agaaccaatg gtgttggtgc caacggcctg accatttttc
      301 ggcgcaggcg catgccacag atcggcaacc atatttacgc aacgcaggtc gatggctgcg
      361 gattgcggat attcttcttt gtcgatccag tttttattga tcgacaaatc catcaattta
      421 tggacgtttt cgtcgtccca ggtctggcag aaagtggcca ggttctgacg agcgttgcca
      481 tcaagatata attcatcatt gataatctgg aatgcgacat catcgcgcat ttcgtgcagc
      541 ggaaatcgtt ttgactccgc gatagtagaa atggcctttg cgccaaaacg tgaatcgagt
      601 agttctgagc ggaaatccgt taacagcttc tggtccattt cgaactcctt aaatttattt
      661 gaaggcaata aaaaagtagg atttatccgc aatggaagca aggcat
//
LOCUS       DAZKIV010000417          703 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZKIV010000417 DAZKIV010000000
VERSION     DAZKIV010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.420"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..252
                     /locus_tag="ACHOT6_005092"
     CDS             <1..252
                     /locus_tag="ACHOT6_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9950092.1"
                     /translation="GASMILGGVAQMLAPKPKTPEYRATDNGKQNTYFSSLDNMIAQG
                     NPMPVPYGEMLVGSRRISQDISTRDEGGDGKVVVIGRQG"
     gene            499..>703
                     /locus_tag="ACHOT6_005093"
     CDS             499..>703
                     /locus_tag="ACHOT6_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950093.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPVEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVW"
BASE COUNT          192 a          148 c          228 g          135 t
ORIGIN      
        1 ggtgccagca tgatactggg tggtgtggca cagatgctgg ccccgaagcc aaaaacaccg
       61 gaatacaggg caacggataa cggtaaacag aacacgtact tttcgtcgct ggataacatg
      121 attgcccagg ggaacccgat gccggtgcct tacggtgaaa tgctggttgg ctcacggcga
      181 atctcccagg acatcagtac ccgtgatgaa ggcggtgacg ggaaggtggt ggttatcggg
      241 cggcaggggt aaagcataaa aaaatcccgc agtgtatgga ggctgcggga acagaaaatg
      301 aagattaacc acagggagtt ttgtttttat tggcccgaaa aaactgtaac gcccgggaat
      361 gatatctgcc acgggggcgt acagaaaatg tgaagaaatt cagaaatttt attccgtcat
      421 gacacaggca ccctccgggg tgcctgtcgt ttttggggca taaacagatt cagacatcag
      481 acaggagagg gggacagagt gggtaaaggt ggcggcaagg cgcacacgcc ggttgaggca
      541 aaggacaatc ttaagtccac gcagatgatg agcgtgattg atgccatcgg tgagggaccg
      601 gtggaaggtc cggtgaaggg gctgcagagt attctggtga acaaaacccc gctgacggac
      661 acggacggta atcctgtgat acatggtgtg acagcggtct ggc
//
LOCUS       DAZKIV010000418          702 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZKIV010000418 DAZKIV010000000
VERSION     DAZKIV010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.421"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>702)
                     /locus_tag="ACHOT6_005094"
     CDS             complement(<1..>702)
                     /locus_tag="ACHOT6_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFP9950094.1"
                     /translation="STKNWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLD
                     YQFSTNEIAAVVAERDVEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKA
                     IEMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDT
                     VFIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQK
                     HYMAGIFNYLKYYKE"
BASE COUNT          219 a          130 c          106 g          247 t
ORIGIN      
        1 tttctttata atactttaaa taattaaaaa tgcctgccat ataatgcttt tgtttaaatt
       61 tatgttttgc acaataccga tactgattgc tatttacagg tttaagtaaa tcttctttac
      121 tccacggaga agctgctttg gcgatcaaaa aactacgtga caccggatag tcagcccact
      181 catgccaggg ttttgtaggt cccacatagt gtataaacac ggtgtcatca ttgactggat
      241 tatcaacttt gtcttttaat tcatagttaa tactatatcg ggtattatat ttctccgaaa
      301 taaacttcac tttaccattc aataatacat tcagtacatc ttgatcaagg tgggtgattt
      361 tacttaccca atctgggtca cgcaacattt caatagcttt tgacgaaatg ttatttagat
      421 tccactcatc aatatttatc agtaaaaaac cagcattgaa atatccagaa gctaactgtg
      481 gtgtagttaa taccgaggct cgattttgcc accattcaac atctctttca gctacaactg
      541 cggcaatttc attagtagaa aattgataat ctaagagttc tttaatacta cccttgcaag
      601 caatatctgc atcaagatat agtatttttt catgtttatg atagaaataa tctgcaatta
      661 taaatcgaaa atatgttgcg taagtccagt ttttcgtgct tg
//
LOCUS       DAZKIV010000419          699 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZKIV010000419 DAZKIV010000000
VERSION     DAZKIV010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.422"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..308)
                     /locus_tag="ACHOT6_005095"
     CDS             complement(<1..308)
                     /locus_tag="ACHOT6_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFP9950095.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     "
BASE COUNT          202 a          182 c           98 g          217 t
ORIGIN      
        1 ttttttgacc gcgcgtttcc ggacgtattc tgttctcctg tccctttata tcgtcggaat
       61 acccgccgct cttcaaatcc cattcccaac tcagaatgta gtctgttgac cgcttgtttt
      121 atttcggtca ggttcaccgg tgaaaccgga gtccggcgcg ccttacgcaa acactctgct
      181 cgtttctgtg ccgccacttt tcttttctgg tcatcactta gctgtaccat cacttttgcc
      241 catcgttcag ctgctctccg gtacagtcct tttttctcca gacattctgc cacgtgatca
      301 tgtagcataa gtgacctccg attatctaca gactgccatc ctgaatttac cttcccttaa
      361 tgaaataaca ataaaaaaca aaccacgcaa aaacaataaa acaacataca aaaaaactaa
      421 ataataaaaa aataatcacc ttattttatt attttttaag ggagcaatta ctgaacaaaa
      481 aacgctgact atatactcaa aaccaaacaa ctattctgcc aatcaggtat catggcaaca
      541 cacggaatta ccgtgttttt gccttctctg cccatacaat acgggcatat acttcatact
      601 ctattgtaat atttctatcc atgtgcccca ctctatttac ctgtaaataa tattcaaaat
      661 atttatcaca gaaatcgttt ttggccatga actgagcac
//
LOCUS       DAZKIV010000420          698 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZKIV010000420 DAZKIV010000000
VERSION     DAZKIV010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.423"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..556)
                     /locus_tag="ACHOT6_005096"
     CDS             complement(<1..556)
                     /locus_tag="ACHOT6_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HFP9950096.1"
                     /translation="MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKL
                     GFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQ
                     NIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGIIYRLISMFAS
                     LLIIFAIAFFFYKDYASVGRNNSSL"
BASE COUNT          287 a          115 c           94 g          202 t
ORIGIN      
        1 ttaatgaaga gttgtttctg ccaactgatg cataatcctt ataaaagaaa aacgcaatcg
       61 caaatataat caagagagat gcaaacattg atattaaacg atatataata ccctttatcc
      121 atgtctcagg ataacggata tttactaaca acaatagtcc acatggaagt atacccgtaa
      181 ataaaaacca cagtacaaca gacatattga aatatgatgt catttcacct gcatttgttt
      241 caaaaatatt ctgcatcatt gttttatcaa actgcacacc atatttcatg gtggcatatg
      301 taacaatgga actacagata aacagaatac aaaaaaaagg tttaaatata tacctgaatg
      361 agaacggtgt aaaaacaaaa tttaatgcag atatcagaaa gactggaaga gatataataa
      421 atccaagttt cacaccatca aggttattaa taatattaaa cagatgcatc cagacaggga
      481 tattgaacaa tgtggcaaaa acaacatcta tcattaacat caagaaaaga aaattaatac
      541 tgattttttt aatcatctta cccccattaa ttagagccta taattgtccg tatctaaaag
      601 tgatatattt ttataaaacc tggtaaagtg aatactgatt tataccaggt caaacttcaa
      661 cttcatcatc aaaactttat gtcaataaag gcgtttat
//
LOCUS       DAZKIV010000421          696 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZKIV010000421 DAZKIV010000000
VERSION     DAZKIV010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.424"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..608
                     /locus_tag="ACHOT6_005097"
     CDS             <1..608
                     /locus_tag="ACHOT6_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFP9950097.1"
                     /translation="PLERALRALVVRHQALTDMHTQELNRTETAREVQRPSIDAHLLW
                     LEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGLKDRFAHARQ
                     FAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEWGRAFRDRLA
                     ANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHNPVAA"
BASE COUNT          174 a          170 c          225 g          127 t
ORIGIN      
        1 acccgcttga acgcgcgttg cgtgccctgg tagtccgcca ccaggcgctg acagatatgc
       61 acacgcagga actgaatcgc actgaaacgg cgcgggaagt ccagagaccg agcattgatg
      121 ctcaccttct gtggcttgaa gcagagctga agcgtcttga gaagcagata aaagacctga
      181 cagacgatga tccggatatg aaacaccgca ggaaactgct ggaaagcatc ccgggtatcg
      241 gagagaaaac atctgcggta ttgctggctt atatcggtct gaaggaccgc ttcgcccatg
      301 ccagacagtt cgccgctttt gcgggtctga caccacggcg ttatgaatca ggtagcagtg
      361 tgagaggggc gagccggatg agtaaggccg gacatgtgtc gcttcgcagg gcgttgtata
      421 tgcccgcaat ggtagccacc agtaagactg agtggggacg ggcgttccgc gaccgtctgg
      481 cggctaatgg caagaaagga aaggtgattc tcggcgcgat gatgcgcaag ctggcacagg
      541 tggcgtatgg agtgctgaag tcaggcgtgc cgttcgatgc gtcacggcat aatccggtag
      601 cggcgtaaaa atcgcggaag ggatgaaaaa aacagcgcct gacggcgctg tgtctggcat
      661 gcctgcaatc cgggaaaccg gaccaggaaa aaactt
//
LOCUS       DAZKIV010000422          682 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZKIV010000422 DAZKIV010000000
VERSION     DAZKIV010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.425"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..504
                     /gene="espX1"
                     /locus_tag="ACHOT6_005098"
     CDS             <1..504
                     /gene="espX1"
                     /locus_tag="ACHOT6_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFP9950098.1"
                     /translation="KMSLSNITIEKSDLSYGYYFGCVLSNISCFESDLSNTIFSNGEI
                     NNLFIKKSNIFGTSFTNTMIKNLLCEDIMPGRWTTQLVNKHLGYRYTGVFKTLASIDD
                     KPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDKTSSEMRKYLKSINECMLLI
                     KNIVHQD"
BASE COUNT          266 a          116 c          104 g          196 t
ORIGIN      
        1 aaaatgtcgc tatcaaatat aacaatagaa aagagcgacc tttcatatgg gtattatttt
       61 ggttgtgtat tatccaatat atcatgtttt gaaagcgact tatcaaacac catcttttca
      121 aatggagaaa taaacaacct cttcattaaa aaaagcaaca tatttggaac atcgttcact
      181 aataccatga taaaaaattt gctctgtgaa gatattatgc ctggtcgatg gaccactcaa
      241 cttgtcaata agcacttagg ttacagatac acaggagtat tcaaaaccct ggcaagtatt
      301 gatgacaaac cgtcccgttt cgaaattttg ataccattgg tacaaacttt agttcgcgac
      361 aatgtaaagc taaataatga tgtgtataaa gaacttaata agtttatgca cgattacgac
      421 aaaacatcat cagaaatgag aaaatacctt aaaagcatca acgagtgcat gctccttata
      481 aaaaatatag ttcatcagga ttaaaacact caaagaaaga ggtaatttta gaatgaaatt
      541 atctgtgatg attattaata cactgcagag aaataaaaaa caatctgaag catgctctga
      601 aacatcctac tctgtcactc taatgatggc accttgctct tcttataaag aagggaacaa
      661 ttaagaatat taaatcttca aa
//
LOCUS       DAZKIV010000423          673 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZKIV010000423 DAZKIV010000000
VERSION     DAZKIV010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.426"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..183)
                     /locus_tag="ACHOT6_005099"
     CDS             complement(<1..183)
                     /locus_tag="ACHOT6_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950099.1"
                     /translation="MIIDLDTLFPVRKTFTDIVSYCTDPFASVENKVFASLPADVECG
                     DLITSTGAKYESGDDIY"
     gene            complement(180..392)
                     /locus_tag="ACHOT6_005100"
     CDS             complement(180..392)
                     /locus_tag="ACHOT6_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950100.1"
                     /translation="MLFLNDKEQIIKYRDEMLRITPQITEMVAKYAGCSVEEVERCVE
                     KYFSPSSPSTPSLNELIKLKAKEITK"
BASE COUNT          238 a          122 c          116 g          197 t
ORIGIN      
        1 atagatatca tctcctgatt catatttagc gcccgtgctg gtgatcaagt cgccacactc
       61 aacatcagca ggaagagaag caaaaacctt gttttctaca gaggcaaacg gatcagtaca
      121 ataacttaca atatctgtga aagtctttcg aactggaaac agagtgtcta aatcaataat
      181 catttagtaa tctccttagc ttttaatttg attaattcat taagggaagg tgtagacgga
      241 gaagaaggag aaaaatattt ttcaacacat ctttctactt cctctacaga acacccagcg
      301 tatttagcta ccatttcggt aatttgtgga gtaattcgca gcatttcatc tcgatacttg
      361 atgatttgct ctttatcgtt taaaaacaac atttatataa accttgttac cttgtttaaa
      421 gtaagaaata caacttttaa gttgcataac gggcaaaata aaaggcagca tttaaagccg
      481 ccttttatta actcatacct atgtaattac cctatgttat gcatactcca agatgtatgc
      541 ataacaaagg aaaaaagagg taataatgac acttaaggag gaggagaagc ggggagcgga
      601 gatttactcc cctttaatga caggaagaat ttttaatatc gaaccaataa aggaaaaata
      661 atcaccccta tat
//
LOCUS       DAZKIV010000424          670 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZKIV010000424 DAZKIV010000000
VERSION     DAZKIV010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..670
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.427"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..603
                     /locus_tag="ACHOT6_005101"
                     /pseudo
     CDS             70..603
                     /locus_tag="ACHOT6_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498860.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          195 a          135 c          164 g          176 t
ORIGIN      
        1 cccgggttaa gggaataagc ctgattctgc ggggtgattt tcaccagtca acaaaatatc
       61 cgtagcgcga taacggtcaa aaattatggc gctgacactt ttgtgccact ggagatgact
      121 gtacctaagt tcaggggaga agaacacgtc cggtgggatg gtcgggccag atttaaaggg
      181 caggtcatgg ctccagcctg tacgctggca atggaggctg cctggcggga aattgatatg
      241 ggaaccacgc cactcaggga tttactgccg gtccagagaa taaattcctg ttacggttac
      301 accactgtga tcttgcaagt gcaggaaagt aggtctacac ggtaacgcga gtgcgtgtaa
      361 cttttgatgt cattcccgta gaaacaccgg acaaattttc gctgacaggt catgcagaag
      421 gtataaatct gcagattatg gacaattacg gatatccggc aagagccgga aaaagcatgc
      481 cgcctctaat tctcagtgga agatggactt gattatactc attgcattgt cagaaatagt
      541 tatccattaa aggctggcta ttccaaacag gatgttgatt acaaaaatgt aatcaacatg
      601 taaggtttat actcttcaat atgcgtataa ttttccttat tttgttaact ttaaataaca
      661 agctatgcac
//
LOCUS       DAZKIV010000425          667 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZKIV010000425 DAZKIV010000000
VERSION     DAZKIV010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.428"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>667
                     /locus_tag="ACHOT6_005102"
     CDS             <1..>667
                     /locus_tag="ACHOT6_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFP9950101.1"
                     /translation="GDSTLSAFNKTLVLSGNQSGLTADRMLTLSRAGQAAGLTFNQAS
                     ESLAALVNAGVRGGEQFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGLMAMAR
                     QFRNVTAEQIAYVAQLQRSGDEAGALQAANDAATKGFDEQTRRLKENMGTLETWADKT
                     GKAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTASFRANL
                     QGAW"
BASE COUNT          147 a          164 c          221 g          135 t
ORIGIN      
        1 gggggattcc acgctttcag cgtttaataa aaccctggtt ctttccggta atcagtccgg
       61 actgactgcc gatcgcatgc tgacgctttc cagagccgga caggccgcag ggctgacgtt
      121 taaccaggcg agtgagtcac tggcagccct ggtgaatgcc ggtgtgcgtg gtggtgaaca
      181 gtttgatgcc atcaaccaga gtgtcgcgcg ttttgcttct gcatccggtg tggaggtgga
      241 caaggttgca gaggctttcg gaaaactgac caccgacccg acgtcggggc tgatggcgat
      301 ggcgcgccag ttccgtaacg tgacggcaga gcagattgcg tatgttgcgc agctgcagcg
      361 ttccggtgat gaggccgggg ccttacaggc ggcgaacgat gctgccacga aaggctttga
      421 tgagcagacc cgtcgcctga aagaaaacat ggggacactg gagacctggg cggataaaac
      481 agggaaggca ttcaaatcga tgtgggatgc cattctggat atcggtcgtc ctgagtcctc
      541 agcggatatg ctcgccagtg cacagaaggc atttgatgag gcggataaaa aatggcagtg
      601 gtaccagagc cggagccagc gccggggaaa aaccgcctct tttcgtgcga accttcaggg
      661 ggcatgg
//
LOCUS       DAZKIV010000426          656 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZKIV010000426 DAZKIV010000000
VERSION     DAZKIV010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.429"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..595)
                     /locus_tag="ACHOT6_005103"
     CDS             complement(<1..595)
                     /locus_tag="ACHOT6_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFP9950102.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSYLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFN"
BASE COUNT          190 a          120 c          133 g          213 t
ORIGIN      
        1 cattaaaatg gcagccatta atttccgcaa cagtcttgta atccttgaaa aatggtttcg
       61 gttgattatt attccatagc caatatgcag aatatgcgcc tatgacaaat gcgctcaaca
      121 taattagata actttttata tgtttgagga ttttgttatt gaaattcttt gcgccaccaa
      181 tgctctcttt gtatcccgca ttaactttta tggcaggtgg ggcattgtgt gtatgtgcgt
      241 ctgttgatga gtttattaca tgattttgta tgttaatgtc attatggatc ttgaatcctc
      301 ttctcggtac ggttgcaata agcgagtggg tagtatcacc aacagcgcga aatccgcgtc
      361 tgataatgga tatgttctga tataacgtgt tagtaggcac acgcatacct tcttcttccc
      421 agactctggt gaaaaaatct tgttgtgcaa ccacatcagg aaaagcttca agtagaagtg
      481 ataaacaacg actggctggc gttgtcagaa cgacgttaag atcggcgtta tgtaccgata
      541 ttaattttcg gtgctcaggc cagaactcga tattatcgtt aataatccag tacatacgtc
      601 aataaacact aaatcaatcg aaatggaaat cacatagttt gctaagtata ttggta
//
LOCUS       DAZKIV010000427          644 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZKIV010000427 DAZKIV010000000
VERSION     DAZKIV010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..644
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.430"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..197
                     /locus_tag="ACHOT6_005104"
     tRNA            122..197
                     /locus_tag="ACHOT6_005104"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
     gene            382..>644
                     /locus_tag="ACHOT6_005105"
     rRNA            382..>644
                     /locus_tag="ACHOT6_005105"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          187 a          142 c          166 g          149 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
      361 tgaaacatct tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag
      421 tcagaggcga tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt
      481 tataaccgac gatttccgaa tggggaaacc cagtgtgttt cgacacacta tcattaactg
      541 aatccatagg ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa
      601 gaaatcaacc gagattcccc cagtagcggc gagcgaacgg ggag
//
LOCUS       DAZKIV010000428          642 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZKIV010000428 DAZKIV010000000
VERSION     DAZKIV010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.431"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..161)
                     /locus_tag="ACHOT6_005106"
     CDS             complement(<1..161)
                     /locus_tag="ACHOT6_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9950103.1"
                     /translation="MSQPAGDLVIDLSLDAARFDEQMARVRRHFSSLEADARKTASTV
                     EQGLSRQALA"
     gene            complement(142..567)
                     /locus_tag="ACHOT6_005107"
     CDS             complement(142..567)
                     /locus_tag="ACHOT6_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFP9950104.1"
                     /translation="MAREMGRPDWRAMLAGMTSTEYADWRRFYCTHYFQDTQLDAHFS
                     GLMYAVLSLFFGDPDMHPADFSLLAPACEEEQTEMPDEEEMLMQKATGVAGGVRFGGD
                     GGRDISPSADVVDVSEDDVALMMASAGISGGVRYVPASG"
BASE COUNT          141 a          212 c          146 g          143 t
ORIGIN      
        1 gccagcgcct gtcggctcag cccctgttca acagtactgg cggtttttct ggcatccgcc
       61 tccagactgg aaaaatgacg gcgtacccgg gccatctgtt catcaaaccg ggccgcatcc
      121 agactcaaat caataaccag atcacccgct ggctgggaca tatctcacac ctccggaaat
      181 ccccgctgaa gccatcatta atgcaacatc atcctcgctg acatccacca catccgcaga
      241 aggtgaaata tcgcgccctc cgtcccctcc gaaccggacg cctccggcaa ctcctgtcgc
      301 tttctgcatc agcatttctt cctcgtccgg catctccgtc tgctcttcct cacacgctgg
      361 agcaagcaga ctgaaatccg ccggatgcat atccggatcg ccaaaaaaca ggctgagtac
      421 ggcgtacatc agcccggaaa aatgagcgtc cagttgggta tcctgaaaat aatgcgtgca
      481 gtaaaaacgt cgccagtcgg catattcggt ggatgtcatc ccggcaagca tggcgcgcca
      541 gtcgggtctc cccatctctc gcgccagttt caggacaaag ttcagctcgc cttcgaatgc
      601 ttttttgatg ttaccggctc agtcgcttct gctttcccgg tt
//
LOCUS       DAZKIV010000429          630 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZKIV010000429 DAZKIV010000000
VERSION     DAZKIV010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.432"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..568
                     /locus_tag="ACHOT6_005108"
     CDS             26..568
                     /locus_tag="ACHOT6_005108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFP9950105.1"
                     /translation="MSESLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGD
                     EDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQ
                     TDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESP
                     SKDATPGNVYAQADYKIAYE"
BASE COUNT          191 a          133 c          142 g          164 t
ORIGIN      
        1 acttatattt atataaagga atattatgtc tgagtcactt cttgttgctg gcgtagcaat
       61 gattctttcc aataacgtat ttgctgacga aggtcacggc attgttaaat ttaaaggtga
      121 agttatctct gctccatgtt caattaaacc aggtgacgaa gacttaactg taaatctggg
      181 tgaagtagca gataccgttc tgaaaagcga ccagaaatct ttggctgagc ctttcactat
      241 tcacctgcaa gactgcatgt tgagccaggg tggaaccact tattcaaaag cgaaggttac
      301 tttcaccaca gcgaatacta tgacgggtca gactgacctg ctgaaaaaca ccaaagaaac
      361 tgaaatcggt ggtgccacag gcgttggcgt acgtattctg gatagccaga gcggcgaagt
      421 gactttaggt actccggtag taattacatt caacaatact aacagctatc aggaactgaa
      481 cttcaaagct cgtatggaat ccccaagcaa agatgctacc ccgggcaacg tttacgctca
      541 ggctgactat aagattgctt acgagtaatc acattaggcc gggttcgccc ggccacatca
      601 ttaacggatt aatgataagt ggaacagatg
//
LOCUS       DAZKIV010000430          627 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZKIV010000430 DAZKIV010000000
VERSION     DAZKIV010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..627
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.433"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>627
                     /locus_tag="ACHOT6_005109"
     CDS             <1..>627
                     /locus_tag="ACHOT6_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9950106.1"
                     /translation="QVYGVRKVWRQLLREGIRVARCTVARLMAVMGLTGVLRGKKVRT
                     TVSRKAVAAGDRVNRQFVAERPDQLWVADFTWVSTWQGFVYVAFIIDVFAGYIVGWRV
                     SSSMETAFVLDALEQALWARRPSGTIHHSDKGSQYVSLAYTQRLKEAGLLASTGSTGD
                     SYDNAMAESINGLYKAEVIHRKRWKNRTEVELATLTWVDWYNNRRLLER"
BASE COUNT          144 a          147 c          200 g          136 t
ORIGIN      
        1 caggtatacg gtgtgcgtaa agtctggcgt cagttgttac gggaaggtat cagagtggcc
       61 agatgcactg tggcacgtct catggcagtt atgggactta ccggtgttct ccggggtaaa
      121 aaggtccgca ctaccgtcag ccggaaagcc gttgccgcag gcgaccgcgt aaaccgtcag
      181 ttcgtggcag aacgtccaga ccagttgtgg gtggctgatt ttacctgggt aagcacatgg
      241 cagggcttcg tttatgtggc gttcatcatt gatgtgttcg ccggatacat cgtgggatgg
      301 cgggtctcat catccatgga aacggcattc gtgctggatg cactggagca ggcgttgtgg
      361 gcccgtcggc cgtccggcac aatccaccac agtgataaag gttctcagta tgtatcgctg
      421 gcctacacgc agcggcttaa ggaagccggc ttactggcat caacagggag tactggtgac
      481 tcgtatgaca acgcgatggc ggagagcatc aatggccttt acaaagcgga ggtaatacac
      541 cgtaagcgct ggaaaaaccg gacagaagtg gagctggcca cactcacgtg ggtggactgg
      601 tataacaatc gacgattgct ggaaagg
//
LOCUS       DAZKIV010000431          624 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZKIV010000431 DAZKIV010000000
VERSION     DAZKIV010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..624
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.434"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>624
                     /locus_tag="ACHOT6_005110"
     CDS             <1..>624
                     /locus_tag="ACHOT6_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950107.1"
                     /translation="GDIYNCTTNTDNAKTPDTPKWPCDNWEEQQPINSTFSGEGYISD
                     QYDLAQHQLQQINACHTNTTYTNADYSKVVAQLVSLITNIETISSTQLTQQTQSILNQ
                     INNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSVQTMFSDTNFIAEQR
                     ADLPTNIKDIQSLYQKMTKLYIEHSENKNRMKVFAGTNFIDFNMTGQN"
BASE COUNT          248 a          125 c           97 g          154 t
ORIGIN      
        1 ggcgatattt ataactgcac aacaaatacc gataatgcta aaacgccaga cacaccgaag
       61 tggccttgcg ataattggga agaacaacaa cccattaatt ccacattctc tggagaagga
      121 tatatctctg atcaatatga tttagcgcaa caccaacttc aacaaattaa tgcgtgccac
      181 acgaacacta catatacaaa tgcagactac tcaaaagttg tggctcaact tgtaagtctt
      241 attactaaca ttgaaacgat aagttcgaca caacttacgc aacagacaca atcaatactt
      301 aatcagatta ataacattag atatgaaaaa aacaaaagtg ctgaatgtcg aatcattgtt
      361 gtcgctaatc ctaaacccga caaggcaatc ataactaaaa ttagcgtaga agagggaata
      421 cccataacat tcagtgtcca aaccatgttt tcagatacta attttattgc tgaacaacga
      481 gctgacttac caactaatat aaaagatatt caaagcctgt atcaaaaaat gacgaagtta
      541 tatattgagc acagtgaaaa taaaaacagg atgaaagtct ttgccggaac caactttatt
      601 gatttcaata tgacaggaca gaac
//
LOCUS       DAZKIV010000432          623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZKIV010000432 DAZKIV010000000
VERSION     DAZKIV010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.435"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..144
                     /locus_tag="ACHOT6_005111"
                     /pseudo
     CDS             1..144
                     /locus_tag="ACHOT6_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(339..506)
                     /locus_tag="ACHOT6_005112"
     CDS             complement(339..506)
                     /locus_tag="ACHOT6_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFP9950108.1"
                     /translation="MTVKLRLTVAALLLFLVVMVDFTSRIMSVLADGVLVCGIMVLLW
                     PVIKRNSLHNA"
     gene            complement(503..>623)
                     /locus_tag="ACHOT6_005113"
     CDS             complement(503..>623)
                     /locus_tag="ACHOT6_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFP9950109.1"
                     /translation="DVLDVAVAIAEADGEIEPEERKVLEEIAGVLGLRLENYL"
BASE COUNT          185 a          178 c          131 g          129 t
ORIGIN      
        1 aacatcgtaa tgtttaatcg acatcgaatt tctcccgtgt agaggaacag agttaaaaag
       61 ccggaagcgg aatcaaatca caggatgacc atctgccagt ggctggtcgt aaaaaaaagg
      121 ccgcgccatg cgcagccgaa aataaaggga taacgatgat agtttgagaa aaacagaaat
      181 aacacttttg tggcaaagca tggtgccggg tgcctcccgg tgaattcagt atcagcacct
      241 gaatccgcga ttaccccata ttccttcttg ctgattgccc caccgcacag ggggattcac
      301 catgcagaag tgtttttaat aaacagcaaa caaaaaaatc aagcattatg caggctgttt
      361 ctttttatca ccggccacag caataccata atgccgcaga ccagcacccc atccgccagc
      421 accgacatga ttctgctggt gaaatccacc atcaccacca gaaacagcag gagtgcagcc
      481 acagtcaggc gcagttttac cgtcacaggt aattctccag acgaagaccc agaacaccgg
      541 caatctcttc cagcaccttg cgctcttccg gctcaatttc gccgtctgcc tccgcaatgg
      601 ccaccgccac atccagcaca tct
//
LOCUS       DAZKIV010000433          623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZKIV010000433 DAZKIV010000000
VERSION     DAZKIV010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.436"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            20..>623
                     /locus_tag="ACHOT6_005114"
     CDS             20..>623
                     /locus_tag="ACHOT6_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9950110.1"
                     /translation="MRHLYGAILFWTALLTGISLPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQLSERVSLYGMAGMAYTRWSDSVQDYRRDEVKPGYVKETTTASDGHT
                     ARHLSPAWNAGIQFSPVETVVIDLAYEGSGSGDWRTDGFIV"
BASE COUNT          132 a          148 c          194 g          149 t
ORIGIN      
        1 ctgaaaacga gaggtcatca tgcgacattt atacggagcc attttattct ggaccgcact
       61 gctgacaggc atttcccttc ctgcacaggc tgaaagcgga cacggtgcat tttccgtggg
      121 atatgctcag gttcacccgg gcggcgtacc ggcattgtcc ggtaccggtg cgcgtgcagg
      181 tgatttaaaa gggattaatg tgaaataccg ttatgagttc acggatcacc tgggcggcat
      241 tgtcgcgctg agttatgcat cggtgaagaa aagtgacacg atgaagacgg gtgaaaatac
      301 cttccattat gaaagcctgc gcggtcgtta tgtcagtctg atggccggcc ctgtctggca
      361 gctcagtgag cgggtcagtc tctatggcat ggccgggatg gcgtacaccc gctggtctga
      421 cagtgttcag gattaccggc gtgatgaagt gaaaccgggg tatgtgaagg agaccaccac
      481 cgccagtgat ggtcatactg cgcgtcatct gtcgccggcc tggaatgccg ggattcagtt
      541 cagtcccgta gagacggtgg ttattgacct tgcttatgaa ggctccggca gtggcgactg
      601 gcgcactgac ggtttcattg tgg
//
LOCUS       DAZKIV010000434          623 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZKIV010000434 DAZKIV010000000
VERSION     DAZKIV010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.437"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..623)
                     /locus_tag="ACHOT6_005115"
                     /pseudo
     CDS             complement(1..623)
                     /locus_tag="ACHOT6_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
BASE COUNT          202 a          121 c           93 g          207 t
ORIGIN      
        1 tcatcttgaa caggttgccc agcattctcc acatcttcaa gccacagcag aagacgtaat
       61 atttcatcta tattttcgaa gctaacataa tcgtagcgac gatgggtagc ataaattttt
      121 cgtgctaatt ttttatctgt gataattggt attccgattt cctttgcata tttccttacg
      181 cctaaggcca cctcattagt tactcttaca gaaatcaatg gaataggtga aagatgtggc
      241 ttaaagtata tccctattgc aatgtgagta gggttggaaa tcatcaaacg actattactg
      301 acatcagact tcaattgctc agaaagaatt tcctgatgcg ctctctgcgt ttagacttaa
      361 tttctggatt tccttcttgc tccttgtatt ctcttttaac ttcttgttta tccattttca
      421 tatctttcat aaataaaaaa tattcagcga taaaatcaaa aattaagaca attatcatac
      481 tgccgagaca atacagtatg agaaggaata gcatctctcc ccaatctgat aataaagata
      541 tgatatctcc atcaagagtt ttaaaaagca gtgatttatg attactccaa aatactttga
      601 tcgccaatgc aaaaaaaaca ata
//
LOCUS       DAZKIV010000435          618 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZKIV010000435 DAZKIV010000000
VERSION     DAZKIV010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..618
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.438"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>618
                     /locus_tag="ACHOT6_005116"
     CDS             <1..>618
                     /locus_tag="ACHOT6_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001567699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFP9950111.1"
                     /translation="PALEQVLYSGMVGEGKHVYTFEDKFKKLFGINNAIAVSSGTAAL
                     HMALILAGIKKGDEVITTSMTAEPTNTSILQAGGVPVFADVDKNTGCLDPDSIIEMIS
                     DKTKAILLVHYAGYLVDVPELKEKLRRKINRKIKIIEDCAHALGATIDGRMVGTFGDY
                     GIFSFQAIKHMTTIDGGFLVIKDTSQTKLAKKMRWFGMEKGVERTS"
BASE COUNT          209 a           92 c          141 g          176 t
ORIGIN      
        1 cctgctttag aacaggttct ttacagtgga atggtcggtg aaggtaagca tgtttatacc
       61 tttgaagata aatttaaaaa gctctttggg attaataatg ctattgctgt tagtagcggt
      121 actgcggcat tacacatggc attaatcttg gctggtataa aaaaaggcga tgaagttatt
      181 accaccagca tgacagcaga accaacaaat acatcaattt tgcaagcagg aggggttcct
      241 gtttttgctg atgtagataa aaacacagga tgtcttgatc ccgattctat tattgagatg
      301 ataagcgata aaactaaagc tatattactt gtccattatg caggatatct ggttgatgtt
      361 cctgaactaa aggaaaaatt aagaagaaaa ataaatagaa aaattaagat tattgaagac
      421 tgtgcacacg ctttaggcgc gacaattgat gggagaatgg taggaacctt cggtgactat
      481 ggcatattct cgtttcaggc tatcaaacat atgacaacaa tcgatggtgg gtttcttgta
      541 attaaggata cctctcagac gaaattggct aaaaaaatgc gatggtttgg catggaaaag
      601 ggagtggaaa gaacatct
//
LOCUS       DAZKIV010000436          616 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZKIV010000436 DAZKIV010000000
VERSION     DAZKIV010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.439"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..324
                     /locus_tag="ACHOT6_005117"
     CDS             <1..324
                     /locus_tag="ACHOT6_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFP9950112.1"
                     /translation="REDDWWRNGQNLYLDNMAVTGFYRVPLSSAQAGDILLCCFGASV
                     PNHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHASAFTGIYNDLAA
                     ASACM"
     gene            321..>616
                     /locus_tag="ACHOT6_005118"
     CDS             321..>616
                     /locus_tag="ACHOT6_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFP9950113.1"
                     /translation="MNTAAEAIRALSLQVPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGASMAAWGAALSAG"
BASE COUNT          116 a          171 c          202 g          127 t
ORIGIN      
        1 cgtgaggatg actggtggcg caacggtcag aacctttacc tggacaatat ggcggtcaca
       61 ggcttttacc gggtgcccct gtcctctgca caggcgggcg atattctgct gtgctgcttt
      121 ggtgcttcgg taccgaacca tgccgccatt tactgtggca acggtgagct gcttcaccat
      181 ctgcctgaac aactgagtaa acgggagagg tattccgaaa aatggcaacg acgaacgcat
      241 tctgtctggc gtcaccgcca ctggcacgca tctgccttca cggggattta caacgatttg
      301 gccgccgcct cagcctgtat gtgaacacgg cagcggaagc catccgggcg ctgtcgttac
      361 aggtgccggg attccgccgt cagatgaacg aaggctggta ccagatacgt attcgcggtg
      421 aggacacggc accggaggcg gtgtacgccc gtcttcacga acctctgggt gagggggcgg
      481 tcatccatat tgtgccgcga ctggccggag ccggaaaggg cggactgcag attgtgctgg
      541 gggcggcagc catcgtgggc tctttcttca cggccggagc atcgatggca gcctggggtg
      601 cagcgctgag tgccgg
//
LOCUS       DAZKIV010000437          612 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZKIV010000437 DAZKIV010000000
VERSION     DAZKIV010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.440"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>612
                     /gene="yfdF"
                     /locus_tag="ACHOT6_005119"
     CDS             <1..>612
                     /gene="yfdF"
                     /locus_tag="ACHOT6_005119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFP9950114.1"
                     /translation="SNDTDHITIGIDNQEPIELPANYKDKELVRTIINDNIVEKTHDI
                     NNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLFTWYGKDKKGD
                     SLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARNASAWFESSKA
                     IMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVD"
BASE COUNT          227 a          109 c          122 g          154 t
ORIGIN      
        1 tccaatgata cagaccatat tactattggt atcgacaacc aagaaccaat agaattacct
       61 gcgaactata aagacaaaga actcgtccgt actatcatta atgacaacat agttgagaag
      121 actcatgata tcaataacaa ggaaatgatc ttcagcgcat taaaagaaat atatgatgga
      181 gatcctggtt ttatcttcga taagatatca cacaaactca gacatacggt aacggaattt
      241 gatgagagcg ggaatagcga accaacggac ttatttacct ggtacggtaa agataaaaaa
      301 ggcgactctc tcgctattgt aattaaaaat aaaaacggaa atgattactt atctctcggt
      361 tactacgatc aggacgacta ccacattcaa agaggaattc gtattaatgg tgatagtctc
      421 acccaatatt gtagtgaaaa agccaggaat gcttcagcgt ggtttgaaag cagtaaagct
      481 atcatggcag aatcatttgc aacaggttcc gatcatcagg ttgtaaacga gctcaacggg
      541 gaaagactga gagaacctaa cgaagttttt aaacgtttag gtcgagcaat aagatataat
      601 tttcaagtgg ac
//
LOCUS       DAZKIV010000438          605 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZKIV010000438 DAZKIV010000000
VERSION     DAZKIV010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.441"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     repeat_region   79..473
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=79..107
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT          164 a          142 c          145 g          154 t
ORIGIN      
        1 ttgtttgtgt gatactgtaa agttggtaga ttgtgactgg cttaaaaaat cattaattaa
       61 taataggtta tgtttacaga gttccccgcg ccagcgggga taaaccgaaa ccggacgagg
      121 gcgcgctaaa acgtgattag agttccccgc gccagcgggg ataaaccgcc taatttttat
      181 atagctgcgg atgatgtaaa gagttccccg cgccagcggg gataaaccgg attttaataa
      241 ctgttgatct actccttgca agagttcccc gcgccagcgg ggataaaccg gcgttgatgt
      301 tcttgcgggt gttgcaaata ttgagttccc cgcgccagcg gggataaacc gtttcagcag
      361 ttcagcgtaa caccgacggt cacgagttcc ccgcgccagc ggggataaac cgcccggaat
      421 gcattctgaa ggtttgctgt atatgagttc cccgcgccag cggggataaa ccaccatata
      481 acccgttatc tctttctcaa gtttttatat tagcagtact tgtaataagc aacatatcca
      541 cgtaacacct catgttcaaa atagttctcc atgccagaga ggttcacaat tatcgataca
      601 aaaaa
//
LOCUS       DAZKIV010000439          602 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZKIV010000439 DAZKIV010000000
VERSION     DAZKIV010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.442"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>602
                     /locus_tag="ACHOT6_005120"
     CDS             <1..>602
                     /locus_tag="ACHOT6_005120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9950115.1"
                     /translation="QEDQAHAALLALETELRTLEKHSGANEKISQQRRDLWKAENQYA
                     VLKEAATKRQLSEQEKSLLAHEKETLEYKRQLAELGDKVEHQKRLNELAQQAARFEQQ
                     QGAKQAAISAKARGLTDRQAQRESEEQRLRDVYGDNPAALAKATSALKNTWSAEEQLR
                     GSWMAGLKSGWGEWAESATDSFSQVKSVATQTFDGIAQNMA"
BASE COUNT          156 a          152 c          204 g           90 t
ORIGIN      
        1 caggaagacc aggcgcatgc tgccctgctg gcgcttgaaa ccgagctccg gacgctggaa
       61 aaacacagcg gtgcgaatga gaaaatcagc cagcagcgtc gcgatttatg gaaagcggaa
      121 aatcagtatg cggtcctgaa agaggcagcc acgaaacggc agttatctga gcaggaaaaa
      181 tccctgctgg ctcatgagaa agagacgctg gagtacaaac gccagctggc tgagcttggc
      241 gacaaggttg aacaccagaa acggctgaat gagctggcac agcaggcggc gcggtttgag
      301 cagcagcagg gcgcgaagca ggcagccatc agcgcaaaag cccgcggcct caccgaccgt
      361 caggcgcagc gggagtcgga agagcagcgc cttcgtgacg tgtacggtga taatccggct
      421 gcgctggcga aggccacatc tgcactgaag aacacctggt ctgcggagga gcagcttcgt
      481 ggaagctgga tggccgggct gaagtccggc tggggcgagt gggcagaaag tgcgacggac
      541 agtttttcgc aggttaaaag cgtggccacg cagacctttg acggtattgc acagaatatg
      601 gc
//
LOCUS       DAZKIV010000440          600 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZKIV010000440 DAZKIV010000000
VERSION     DAZKIV010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.443"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(96..>600)
                     /locus_tag="ACHOT6_005121"
     CDS             complement(96..>600)
                     /locus_tag="ACHOT6_005121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFP9950116.1"
                     /translation="HETAVVRRKSEASRFQDFVACGDMTIVRRVGDDTAEVAEYVRRI
                     HEAELLEHIGIDPSGVGQILDSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVL
                     VHGGQPLMAWCVGNARVEPKGNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKK
                     EYAVFFI"
BASE COUNT          136 a          198 c          137 g          129 t
ORIGIN      
        1 gtcagcaggg agcaggcccg tttaagagtc acatcagcct cctgaaaata aaaaaaccgc
       61 cgcagcggtc catgatggtt acagggtgaa cagggttata tgaaaaaaac cgcatattct
      121 ttctttttcg gctccggatt cagggacatc agggataccg cattgaagag cgccatcagc
      181 gggtcaattt tcccccgtcc gctggcctgt ttggtaataa ggatggcgtt acctttcggc
      241 tccacccggg cattgccaac gcaccaggcc atcagtggct gaccaccatg caccagcact
      301 ccctcagcca gtttgcgctc ggtggttttt atggccccgc ccagcttcca gccctggctt
      361 atccccacaa caattccgtc ggggatcccg gcttccgcca gtgaatccag aatctgcccc
      421 acacctgacg ggtcaatacc gatatgctcc agtaactcag cctcatgaat acgacgcaca
      481 tactccgcca cttccgccgt gtcatccccg acccgacgga caatcgtcat gtctccacag
      541 gccacaaaat cctgaaaccg ggatgcctca ctcttccgtc tgaccaccgc ggtttcatgc
//
LOCUS       DAZKIV010000441          595 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZKIV010000441 DAZKIV010000000
VERSION     DAZKIV010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.444"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..71)
                     /locus_tag="ACHOT6_005122"
     CDS             complement(<1..71)
                     /locus_tag="ACHOT6_005122"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950117.1"
                     /translation="MIYTTNAVESLNSVIRHAIRKRKV"
     gene            complement(226..291)
                     /locus_tag="ACHOT6_005123"
     CDS             complement(226..291)
                     /locus_tag="ACHOT6_005123"
                     /inference="COORDINATES: protein motif:HMM:NF033646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAST1 family heat-stable enterotoxin"
                     /protein_id="HFP9950118.1"
                     /translation="MWCATACASCHGRNTKPSLAT"
     gene            complement(275..487)
                     /locus_tag="ACHOT6_005124"
                     /pseudo
     CDS             complement(275..487)
                     /locus_tag="ACHOT6_005124"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          140 a          158 c          146 g          151 t
ORIGIN      
        1 accttgcgtt tcctgatggc atgccggatc acactgttca gcgactcgac ggcgttcgtt
       61 gtgtagatca ctttgcggat atctgccgga taagcgaaaa acgtcgccaa gttagtccag
      121 tttgacagct agcaccggct tatctgcgga tagcggcagt cccaggccgc agcaaacgct
      181 tccagtgcct gctggcctgc ctcttctgtg ggaggctaat cgctttcagg tcgcgagtga
      241 cggctttgta ttccttccat gacacgaagc gcatgctgtt gcgcaccata tgcacgatgc
      301 ataactggat gcgggcctcc gggaattctt tcaggccatc cacacaggcg atgaggatat
      361 cgttcagacc gcggtttttc agttcagtca gcacattgaa ccagaaattc gccccttcat
      421 tttcggccag ccgcatcccc aggagctctt tctgacctgc gatattgatg cccagtgcta
      481 ggaacatttt tatgacaaaa aattagtgca agaagacaaa gaatcgcctt gcgctaatgc
      541 tctgaaacag gtcactaata tcatctaaat agttgactca tagtgacagc atgtg
//
LOCUS       DAZKIV010000442          594 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZKIV010000442 DAZKIV010000000
VERSION     DAZKIV010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.445"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..232)
                     /locus_tag="ACHOT6_005125"
     CDS             complement(<1..232)
                     /locus_tag="ACHOT6_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HFP9950119.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADN"
     gene            complement(275..511)
                     /gene="ycgZ"
                     /locus_tag="ACHOT6_005126"
     CDS             complement(275..511)
                     /gene="ycgZ"
                     /locus_tag="ACHOT6_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFP9950120.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          169 a          127 c          109 g          189 t
ORIGIN      
        1 tattgtcagc tgtcgctgtt cttcggtttg gccctcctga atcactattg ctaaaatcac
       61 gaattatgct gtcaattaaa tcgcgtcgga aagcatcttc ctcagccgat ttcattacga
      121 gtaattgctt aataagagtc tgcaatgaaa aatttatctt catgcgcagt atctggagca
      181 ccgcctggcc agtaatttca tattttatac gttcattatc agatgtcttc ataacgactt
      241 catagctaac ctgttaaata atcaggctgt tgcattattc aaaaagcaac ccaattagtg
      301 cattataatg tttctgttcc tcttcccctg tcgcttgttc cagtcggcaa agaagtttgg
      361 cacaaagcga ctttcgactc agattacgtc catctttcaa aatctcagtc acaatttccc
      421 cgagagtttc ctgctgagta ggcaagttag cttttgcaaa gtaacgagtg attgctcccg
      481 cagaatctga agtcactgaa ttttgatgca tgctacgcct ctgttaaaaa tgttaatcaa
      541 tgtgaagtga ggtaacaata gcaaacttgt acgaaatatg tgtacagaat gata
//
LOCUS       DAZKIV010000443          593 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZKIV010000443 DAZKIV010000000
VERSION     DAZKIV010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.446"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            28..>593
                     /locus_tag="ACHOT6_005127"
     CDS             28..>593
                     /locus_tag="ACHOT6_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFP9950121.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNS"
BASE COUNT          189 a          107 c          119 g          178 t
ORIGIN      
        1 gaaacaatat tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt
       61 acgccgcttt tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct
      121 atggctgttt ccattaatag tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat
      181 gaagttcagt acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa
      241 aatgaagttg agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc
      301 gctttaccag ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa
      361 gagaaaaatt atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat
      421 aaaaaagatc tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt
      481 ccacctcagc aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca
      541 gggaatcaac gcttaaagat tttaacgatc gcttattgta aaaataatag caa
//
LOCUS       DAZKIV010000444          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZKIV010000444 DAZKIV010000000
VERSION     DAZKIV010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.447"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>590)
                     /locus_tag="ACHOT6_005128"
     CDS             complement(<1..>590)
                     /locus_tag="ACHOT6_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950122.1"
                     /translation="RLFAVSITSSGNPPTFSLTPEGKLTARNADISGAITANTGTLNN
                     VTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSYASGTVTVTVYDDQGFDRQIIIP
                     PVLFRGTKHQNFNSPNQQSYWYSTCKLQVLKNGVEIFHEPATDVSRVFSSVIDMPAGR
                     GHVTLTFNVSSAGANNWTPTTYISDLLVVVMKKSTAG"
BASE COUNT          121 a          168 c          152 g          149 t
ORIGIN      
        1 cctgctgtgg attttttcat gaccacaacc agtaaatcac tgatgtacgt tgtcggcgtc
       61 cagttgttcg caccggccga cgacacatta aacgtcaggg tgacatgacc ccgccctgcc
      121 ggcatatcta tcaccgatga gaacacccgg ctgacatccg ttgccggttc atggaaaatc
      181 tcaaccccgt tcttcagcac ctgcagctta caggtggaat accagtacga ctgctgattc
      241 gggctgttga aattctggtg tttcgtcccg cgaaacagca ccgggggaat gatgatctgc
      301 cggtcgaagc cctggtcatc gtaaactgtg acggttaccg tcccgctggc ataactgtta
      361 ttccggggaa aggctttccc caccgtcttc accaggtcgc cttcaatctg gtttgcagac
      421 agtttccctc tgatgacaca gttctcgtta atggtgacat tattgagcgt gccggtattc
      481 gcggtaattg ctccgctgat atccgcgttc ctggctgtca gcttcccttc cggcgtcagg
      541 gaaaacgtcg gggggttgcc ggatgacgtg atactcaccg caaacagtcg
//
LOCUS       DAZKIV010000445          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZKIV010000445 DAZKIV010000000
VERSION     DAZKIV010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.448"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..496
                     /locus_tag="ACHOT6_005129"
                     /pseudo
     CDS             1..496
                     /locus_tag="ACHOT6_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
     gene            498..>590
                     /locus_tag="ACHOT6_005130"
     CDS             498..>590
                     /locus_tag="ACHOT6_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFP9950123.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNIFLSN"
BASE COUNT          228 a           72 c          118 g          172 t
ORIGIN      
        1 ctgtatacat atccgctgag cccggtgaat agcatggatc cattaggatt atatgaattt
       61 aaatcaaaaa atatagatga tattggaata tttgcattgg caatgtgtaa tggagaatca
      121 attaacgaga ataaagaata tggtggacta atatgtaaga agcaaggtga atatttcccc
      181 atgaatccga taagttcaaa tgataatgat agtgtagact tgcgaaatat aaaatgccct
      241 gaaggttcag agagagtagg cgattatcac actcacggtt tttactctga cgataaagga
      301 aataaagtaa caaaagaaaa tgatgtttat gatagtctaa atttttcaag caaagattta
      361 acgaattctt atatgaatgg aatgggaaaa aaaagaatac agtagttact tgggaacacc
      421 aaataacacc tatctaaaat ataatcccaa agctaaaggg aatggagtta caattatcag
      481 gcaagggagt aattaaaatg aaggcaatac aatgttgtta tttccttgct ttagtctcca
      541 gtcatttaat ggctggagtt atgggtgatg ataatatatt tttaagtaat
//
LOCUS       DAZKIV010000446          590 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZKIV010000446 DAZKIV010000000
VERSION     DAZKIV010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.449"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(48..>590)
                     /locus_tag="ACHOT6_005131"
     CDS             complement(48..>590)
                     /locus_tag="ACHOT6_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFP9950124.1"
                     /translation="APAWASEQQATLSAGYLHVSTNVPGSDELNGINVKYRYEFTDTL
                     GMVTSFSYAGDRNRQLTRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAY
                     SRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPIESVAIDIAYE
                     GSGSGDWRTDGFIVGVGYKF"
BASE COUNT          136 a          191 c          142 g          121 t
ORIGIN      
        1 gcccgcctga accggcgggc tgtcataaca ctgtgttacc tggctaatca gaacttataa
       61 ccgacaccca cgatgaaacc gtcagtgcgc cagtcgccac tgccggagcc ttcataagca
      121 atatcaatgg ccacggattc gatcgggtta aactgcacgc cagctcccca cgccagagac
      181 gtgttgctgt ggcgaccgtc atcacttccg gtcagcacat catgcgtttt ccccttgttg
      241 tcagttacgc ggagataatc cccggaaaaa gtcgacacac ggctgtaagc cacgcccgcc
      301 atcgcatacg cactgaacca ttcattcacg cgcacagagg gccccgccat cacgctgaac
      361 cagcggttac gaacggaatc ttcatgccag cgggtatcgc tgtaacgggt aagctggcga
      421 ttcctgtctc ctgcatagct gaatgacgtc accatcccca gcgtatccgt aaactcataa
      481 cggtatttca cgttaatccc gttcagttca tcgctgccag gaacgttcgt cgagacatga
      541 agataccccg cgctcagcgt ggcctgctgc tcagacgccc atgcaggcgc
//
LOCUS       DAZKIV010000447          565 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZKIV010000447 DAZKIV010000000
VERSION     DAZKIV010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.450"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..503)
                     /locus_tag="ACHOT6_005132"
     CDS             complement(<1..503)
                     /locus_tag="ACHOT6_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFP9950125.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIISTKQHFFSSKLFKYVMLFFLFAIIFI
                     AFYIVNA"
BASE COUNT          197 a          106 c           83 g          179 t
ORIGIN      
        1 ctggcattaa caatataaaa agcaatgaaa atgatagcaa aaagaaaaaa aagcattaca
       61 tacttaaaca gcttactgct aaagaaatgt tgttttgtag aaataatgtt attttcaata
      121 atgcctggca atgaatactg ccaaacttca tttttttctt ttacagcaaa tttgagacct
      181 aaaacttcta tttgctgatt caatgttaac gttctatctt caggttcttg tgctgttaat
      241 tctctgaatg ttactaccag atcagggtcg gtagtaatta ttatctcaaa actacctgta
      301 agctcgccat aaggaacgac tattgtgttt tctggtaact cagaaaaagc atcacttctt
      361 cctaacgaaa catcattacc aatgataaca agtagacgcc cattaagtat ttcatactcg
      421 catccattca atggtccgct caacaaacga atggcataag attccaataa gtcttgtgat
      481 aagaatttat cattattttc catgaatacc acccgctaat tccataagct ctagtttaga
      541 gtagtgtaac aagacggaaa tttca
//
LOCUS       DAZKIV010000448          561 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZKIV010000448 DAZKIV010000000
VERSION     DAZKIV010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..561
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.451"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHOT6_005133"
     CDS             <1..112
                     /locus_tag="ACHOT6_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFP9950126.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHOT6_005134"
     CDS             171..374
                     /locus_tag="ACHOT6_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFP9950127.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..>561
                     /locus_tag="ACHOT6_005135"
     CDS             374..>561
                     /locus_tag="ACHOT6_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFP9950128.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKRYEETVNNRDRAMT"
BASE COUNT          183 a          117 c          120 g          141 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac tacctccaaa
      421 tgtataccca aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat t
//
LOCUS       DAZKIV010000449          556 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZKIV010000449 DAZKIV010000000
VERSION     DAZKIV010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.452"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..85)
                     /locus_tag="ACHOT6_005136"
     CDS             complement(<1..85)
                     /locus_tag="ACHOT6_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9950129.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAI"
     gene            complement(178..369)
                     /gene="ydfD"
                     /locus_tag="ACHOT6_005137"
     CDS             complement(178..369)
                     /gene="ydfD"
                     /locus_tag="ACHOT6_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFP9950130.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            complement(366..554)
                     /gene="dicB"
                     /locus_tag="ACHOT6_005138"
     CDS             complement(366..554)
                     /gene="dicB"
                     /locus_tag="ACHOT6_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFP9950131.1"
                     /translation="METLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
BASE COUNT          152 a          119 c          110 g          175 t
ORIGIN      
        1 caatggcagt ggctacagcg actgcacctt cttcctttat tgcctgttcg tccggaatgg
       61 cggcgcaaat aaagacttta ctcattttgt tttacctcat tacagattta agggtgaaca
      121 aatccctgcc attgctggca tataagaatg aaatcggatg tttattacgg aactgtttta
      181 aagacctgcc gggatttcgt tattatcctg gtgaataact ttatcgaccg ggtaacagtt
      241 accgggaatt ttctgttcgg ttgctgcagt cacacactcc tgcattgtcc tgtgaacact
      301 gactgcaata tcaactggct ctccggaaac aagaaaaact gtcagaacaa gtgcaaatgc
      361 tgtattcatt gccagcatcc tttttgtatc ggacgtaaac gggccagcat tgaaagaatg
      421 catattttat ttaatagctc ccgttcgtgt tttctcttgt taatggcatc ttcagtaaat
      481 acagggttac tgatagtgac accaatttca aaacaacctt cagacgtatt aacgtttggt
      541 aataacgttt ccatta
//
LOCUS       DAZKIV010000450          554 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZKIV010000450 DAZKIV010000000
VERSION     DAZKIV010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 554)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 554)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..554
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.453"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..554
                     /locus_tag="ACHOT6_005139"
                     /pseudo
     CDS             1..554
                     /locus_tag="ACHOT6_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Ulp1 family isopeptidase"
BASE COUNT          197 a          120 c           86 g          151 t
ORIGIN      
        1 attatataat ttggcacata ctgcaaccaa ctcccacctt aaaccaactg gtccggataa
       61 ttcagatgtt ctcctgttca aaaagctatt ctccccttcg aaattaatga caattatcgg
      121 tgatgaaatt cctcttatat ctgaaaaaca gtcgctttca aaggtgcttt taaatgatga
      181 gaataatgaa ctgagtgatg gtacaaactt ctgggataaa aatcgtcaat taaccacaga
      241 tgaaatagct tgctatcttc agaagatcgc cgctaatgca aaaaatactc aagtcaatta
      301 tcctactggt ctctacgtcc cctactccac cagaactcac ctggaagacg ctctcaatga
      361 aaatattaag agcgatccat catggccgaa tgaagtccag ttattcccca taaatactgg
      421 cggacactgg atattagttt cgctacagaa aatagtaaat aaaaaaataa taaactacaa
      481 ataaaatgcg tcatattcaa ctcattgcgt gcactaggcc atgataaaga aaattcactt
      541 acgcgtgtca ttaa
//
LOCUS       DAZKIV010000451          552 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZKIV010000451 DAZKIV010000000
VERSION     DAZKIV010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.454"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..95)
                     /locus_tag="ACHOT6_005140"
     CDS             complement(<1..95)
                     /locus_tag="ACHOT6_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950132.1"
                     /translation="MKIPVLQPGFNFFAPAGYSAAVAPNRAENAYA"
BASE COUNT          173 a          124 c           98 g          157 t
ORIGIN      
        1 gcataggcat tttcagcacg attaggagca acggcagcag agtatccagc aggggcaaaa
       61 aagttgaagc caggttggag aacgggaatt ttcatattga tacttaatat ggttttatat
      121 atttcagtgc caccaattca ctcgcttaca accagaaact aaaaaaacgt cagggcgttg
      181 ataataatga atacaaactt tctgcccatt tatagtaata tctttcgtta tcaggttttg
      241 tctggcagct tcacgtaata tttcaatact ctgcaaagca acatttgatg aaatgctgga
      301 tgttatcaat ggcatatgtc atttacccca acaagactaa attttagaaa aaacaactgc
      361 cgggacaact ttcgccccct gtaaccccaa ataagatgtc agatttatcg gcattaattt
      421 atctccctgt tgaattagca cctctatcat ctacttaaca aatatccccc ggacattgca
      481 acacaaaaac cggagccgga ctccggtttt gtgaagctgt cgggttactt catcccgcca
      541 atattttccc ac
//
LOCUS       DAZKIV010000452          550 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZKIV010000452 DAZKIV010000000
VERSION     DAZKIV010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.455"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..218
                     /locus_tag="ACHOT6_005141"
     CDS             <1..218
                     /locus_tag="ACHOT6_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFP9950133.1"
                     /translation="LNAVLIMLAQHALLIAISKDLNAYGVVCEFDWDDGNGQEGWPPM
                     DGGEGIRITDIDTSGIFDSDDMTIKAV"
     gene            291..455
                     /locus_tag="ACHOT6_005142"
     CDS             291..455
                     /locus_tag="ACHOT6_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFP9950134.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNTYGYPD"
     gene            424..>550
                     /gene="kil"
                     /locus_tag="ACHOT6_005143"
     CDS             424..>550
                     /gene="kil"
                     /locus_tag="ACHOT6_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFP9950135.1"
                     /translation="MDQTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILMLKLRS"
BASE COUNT          165 a          106 c          125 g          154 t
ORIGIN      
        1 tattaaatgc tgtattaatc atgctggcgc aacatgctct gcttatagca atttcgaaag
       61 acttaaatgc atatggtgtt gtttgtgagt tcgactggga tgatggaaat ggtcaggaag
      121 gatggcctcc aatggatgga ggtgaaggaa taagaattac cgatatcgat acatcaggaa
      181 tatttgattc agatgatatg actatcaagg ccgtctgagt gcggttttac cgcataccaa
      241 taacgcttca ctcgaggcgt ttttcgttat gtataaataa ggagcacacc ctgcaatatg
      301 ccattgcagg gtggcctgtt gctggctgcc cttccgaatc tttacttgaa cgaatcaccc
      361 gtaaattacg tgacggatgg aaacgcctta tcgacatact taatcagcca ggagtcccaa
      421 agaatggatc aaacacttat ggctatccag actaaattca ctatcgccac ttttattggc
      481 gatgaaaaga tgtttcgtga agccgtcgac gcttataaaa aatggatatt aatgctgaaa
      541 ctgagatcaa
//
LOCUS       DAZKIV010000453          549 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZKIV010000453 DAZKIV010000000
VERSION     DAZKIV010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.456"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..433
                     /locus_tag="ACHOT6_005144"
     CDS             68..433
                     /locus_tag="ACHOT6_005144"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950136.1"
                     /translation="MSRSAKPRKRKPAPQRSKLPRYVVKLHDDDFFDEEDAEALRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILAGISAGG
                     MYCPECGTVHWPDGVPPPF"
     gene            433..>549
                     /locus_tag="ACHOT6_005145"
     CDS             433..>549
                     /locus_tag="ACHOT6_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HFP9950137.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGSVRPVVIESVTKIM"
BASE COUNT          129 a          139 c          142 g          139 t
ORIGIN      
        1 gcggcttgtc atggcctgaa attaccgtta taaaaacaga caatatcatt gtctttcagg
       61 tagttatatg tcccgttcag ctaaaccccg taaacgaaaa cctgcccctc aaagaagcaa
      121 acttccccgc tatgtcgtga agcttcacga cgatgacttc tttgacgaag aagacgcaga
      181 agctctgcgc tttgataatt ttgacgatgc cgttgagtgc tgcgcagacc tgaatattcc
      241 cttctttgtg gatgccggaa acaaaaagct ggtcttctgg tttgtacgtg ttgatgacga
      301 agggtatcct gaaatagccc gctgcacgga gcgggagttt gcgaccattc ttgccggtat
      361 cagcgccggc ggcatgtact gcccggagtg tggcacggtt cactggccgg acggagtccc
      421 cccgcccttc tgatgcttcc ccgttttgcc gacatttttc agcagggaaa ccgctggctt
      481 aactggctgg agaaacaacc ggaaggttca gtgcgtccgg tagtcattga gtctgtgaca
      541 aaaatcatg
//
LOCUS       DAZKIV010000454          546 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZKIV010000454 DAZKIV010000000
VERSION     DAZKIV010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.457"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          163 a           95 c          154 g          134 t
ORIGIN      
        1 aaagtgtaaa aagaatttat atcatgggat gcagtgtaaa tatggatgat aataatcttt
       61 gtgactattt taaatcgcga ggttttacta aaataagata tgaaccaaat aggagtgata
      121 cgctatttga agcaattaga tagggtttgt tccagggaaa gatcacggtg tgccgtaggc
      181 tcaaagatgc aaaacgggac gttttgcatg taataagcat aagcttgtga agagatggtg
      241 gcaggcccac cgggatctgc tgtcggaagt cggtcccaaa caaccccggc tgctgtggta
      301 aagagccatg gtagtgagcg cagggggaac gatcagacga gatctgatcc tatgtgagat
      361 acagtgagat gagtaaaccc gaacctgtgt tgaaacgtcg ttagtcctaa gtgacatcaa
      421 aaccggaggt ttcgtgatac cgccgggatt aatttacggg tgaattccga atgctgcgta
      481 aatggtgtcc ggcgtatagc aggcatgacg ctgtatcagg ctctttgcag gaactacggg
      541 aaccgg
//
LOCUS       DAZKIV010000455          539 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZKIV010000455 DAZKIV010000000
VERSION     DAZKIV010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.458"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(5..>539)
                     /locus_tag="ACHOT6_005146"
     CDS             complement(5..>539)
                     /locus_tag="ACHOT6_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system domain-containing
                     protein"
                     /protein_id="HFP9950138.1"
                     /translation="HDEWLGDLTGVNQVSFRRLRETSSTRNQLNKILRQEIQDKISGV
                     ELSDINKEGFLYQSIGKIRLLALSSALFDIQCPDYIFSRLYRETLIREIGYQNVKQLS
                     FYWQGGQCKPEYGEERFCAELIKYGAGNLEWLFADNPLWTIVKYLLPKSGEIKPTHIN
                     DLFLNRLNKILLPYETL"
BASE COUNT          178 a          107 c           71 g          183 t
ORIGIN      
        1 aaattcataa agtttcataa ggcaataaaa ttttattaag tctgttaaga aataaatcat
       61 taatatgagt tggttttatt tctccacttt tcggtaaaag atatttgact atggtccata
      121 aaggattatc agcaaatagc cactccaaat ttcctgcacc gtattttatt aactcagcac
      181 aaaaccgttc ttcaccatat tctggtttac attgccctcc ctgccaatag aatgataact
      241 gcttcacatt ttgatatcct atctctctaa tcagagtttc tcgatataat cgtgagaaaa
      301 tataatcagg gcattgaatg tcaaataatg cactacttag agcaagcaat cttattttcc
      361 caattgactg atataaaaaa ccttccttat ttatgtcaga taattccaca ccagaaattt
      421 tatcttgtat ttcctgacgc aatatcttat tgagttgatt acgagtagaa ctagtttctc
      481 ttaaacgccg aaaactgacc tgattgacac cggttaaatc acctagccat tcatcatga
//
LOCUS       DAZKIV010000456          512 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZKIV010000456 DAZKIV010000000
VERSION     DAZKIV010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.459"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>512
                     /locus_tag="ACHOT6_005147"
     CDS             <1..>512
                     /locus_tag="ACHOT6_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFP9950139.1"
                     /translation="AASFVPVETESDPMNVAFDKLAPEVQNAVMVKFDTCENITVDMV
                     ISAQELLQEDMATFDGHIVEALMKMPEVNAMYPELKLHAIGWVKHKCKPGAKWPEIQA
                     EMRIWKKRREGERKETGKYTSVVDLARARVHRQHTENSAEKIPPVTAVIRREYKQTWK
                     TLDDELAYAL"
BASE COUNT          150 a          123 c          130 g          109 t
ORIGIN      
        1 ggctgcttcc tttgtcccgg ttgagactga atcagatccg atgaacgtcg cttttgacaa
       61 gctggcccct gaagtacaga acgctgtcat ggttaagttc gacacatgtg aaaacatcac
      121 cgttgatatg gtgattagcg cgcaggaatt gttgcaggaa gacatggcaa cattcgacgg
      181 acatatcgtt gaagcgttga tgaaaatgcc agaagttaac gccatgtatc cggagcttaa
      241 gctgcatgcc atcgggtggg ttaagcataa atgtaagcct ggtgccaaat ggcccgaaat
      301 tcaggcagaa atgcgcatct ggaaaaaacg tcgcgaaggt gaacgcaagg aaaccggaaa
      361 atacacgtct gttgttgatc tcgcccgcgc cagagtccac cgacagcaca ctgaaaactc
      421 agcagaaaaa atcccccctg tcactgcagt cattcgtcgc gaatataagc agacatggaa
      481 aacactggat gacgaactgg cctacgctct ct
//
LOCUS       DAZKIV010000457          490 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZKIV010000457 DAZKIV010000000
VERSION     DAZKIV010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..490
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.460"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(73..243)
                     /locus_tag="ACHOT6_005148"
     CDS             complement(73..243)
                     /locus_tag="ACHOT6_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950140.1"
                     /translation="MSETTEKKRQPRTCFHIPDEVFQVLFKEARSRRITVTDYILDML
                     KREAETLSNKSK"
     gene            complement(263..451)
                     /locus_tag="ACHOT6_005149"
     CDS             complement(263..451)
                     /locus_tag="ACHOT6_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950141.1"
                     /translation="MKWFTPEHVVEAFKKGDLTRHQVVMNRNMARSRGYPERVACFNE
                     ALKIIDELRKNEKESETE"
BASE COUNT          132 a           99 c           73 g          186 t
ORIGIN      
        1 aagttagttg gttaatcatt aaaaatctcc ttgatgtaat cacgctctat ggcgctacta
       61 tggtgataaa aatcacttgc ttttatttga gagtgtttca gcctctcttt tcaacatatc
      121 aagaatgtaa tctgtgacag tgatacgtct acttctggct tctttaaata acacctgaaa
      181 tacttcatca ggaatatgaa agcaagtgcg tggttgtctt ttcttttctg tagtttcgct
      241 catagttttc tccttctatt ctttactctg tttcagattc tttttcattt tttcttaatt
      301 catcaataat cttgagtgct tcattgaagc aagcaactcg ctctggataa cctcgactcc
      361 ttgccatatt acgattcatt actacctggt ggcgtgttag atcacctttt ttaaatgcct
      421 ctacgacgtg ttcaggtgta aaccatttca taaaaaactc cttattcagt taaaagaaat
      481 aaaacacctt
//
LOCUS       DAZKIV010000458          486 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZKIV010000458 DAZKIV010000000
VERSION     DAZKIV010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.461"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..168
                     /locus_tag="ACHOT6_005150"
     CDS             <1..168
                     /locus_tag="ACHOT6_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFP9950142.1"
                     /translation="ARLDAEVDAILDELLGVQAEPPTEAGDTTAESGEVQPESPVADA
                     TEPQPESVMML"
     gene            245..460
                     /locus_tag="ACHOT6_005151"
     CDS             245..460
                     /locus_tag="ACHOT6_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFP9950143.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
BASE COUNT          124 a          108 c          153 g          101 t
ORIGIN      
        1 gcgcgtcttg atgcagaggt tgatgccatt ctggatgagc tgcttggtgt acaggcagag
       61 ccaccgactg aagcgggaga taccaccgca gagagcggtg aagttcagcc tgaatcaccg
      121 gtcgccgatg cgactgaacc tcaacccgaa tcggtcatga tgctgtaacg gggagtcagg
      181 gccatcagta aacagctgct ggcctttttc atgttgtgag cttccggata acgggagacg
      241 gggtatgtac cagatggaaa aaatcacaac aggtgtgtca tacaccacgt cagcggtagg
      301 gacgggatac tggttactgc aactgctgga caaagtctct ccgtcccagt gggtggcaat
      361 aggtgtactg gggagtctgc tgtttggcct gctgacgtat ctgactaacc tgtatttcaa
      421 aatcagagag gaccggcgta aggcggcgcg gggagagtaa agcgatgaag aaaaaatacg
      481 aactgg
//
LOCUS       DAZKIV010000459          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZKIV010000459 DAZKIV010000000
VERSION     DAZKIV010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.462"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>483
                     /locus_tag="ACHOT6_005152"
     CDS             <1..>483
                     /locus_tag="ACHOT6_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFP9950144.1"
                     /translation="ARHAGEAKKNAGEAETSARNAGISAGQAEESAANADTSAGDASE
                     SARQAAESAAAAKQSEEASSSSASAAAQKASESLQSAADAELSKKTAESAAGNAARDA
                     TTATEKARESAESAQSAEQSRIAAEEAVNRIPTVVGPPGPKGEQGPAGPQGPKGDKGE
                     R"
BASE COUNT          135 a          124 c          174 g           50 t
ORIGIN      
        1 agcgcgtcac gccggggagg cgaagaagaa tgccggagag gcggagacgt cagcgaggaa
       61 tgccggcata tcagccggtc aggcagaaga gagcgcggca aatgctgaca cttcagcagg
      121 ggatgcatcg gagtcagccc ggcaggcggc agaaagtgca gccgctgcaa agcagtcaga
      181 ggaggcgtcc tcgtcctcgg cctctgcggc cgctcaaaaa gccagtgagt cattacaaag
      241 tgcagcagat gctgagttgt caaaaaagac ggcagaaagt gcagccggta atgcagccag
      301 ggatgcaacg accgcaacag aaaaagcccg ggagtcagca gaaagcgcac agtcagcgga
      361 acaaagcagg atagcggcgg aagaggccgt aaaccgaatc cccaccgtgg tgggacctcc
      421 cgggccaaag ggggaacagg ggcccgcggg tcctcagggg ccgaagggtg ataagggaga
      481 gcg
//
LOCUS       DAZKIV010000460          483 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZKIV010000460 DAZKIV010000000
VERSION     DAZKIV010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.463"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>483
                     /gene="escU"
                     /locus_tag="ACHOT6_005153"
     CDS             <1..>483
                     /gene="escU"
                     /locus_tag="ACHOT6_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE export apparatus
                     switch protein EscU"
                     /protein_id="HFP9950145.1"
                     /translation="SYANEFANFTGLNAYQALVVVAFFVFLLWKGVLFGYLLFSVFDF
                     WFQKHEGLKKMKMSKDEVKREAKDTDGNPEIKGERRRLHSEIQSGSLANNIKKSTVIV
                     KNPTHIAICLYYKLGETPLPLVIETGKDAKALQIIKLAELYDIPVIEDIPLARSLYKN
                     "
BASE COUNT          156 a           84 c           98 g          145 t
ORIGIN      
        1 attcatatgc aaatgagttt gctaatttca caggactgaa cgcatatcaa gctcttgtcg
       61 ttgttgcctt ttttgttttt cttttatgga aaggcgtgct attcggatat ctactctttt
      121 cagtatttga tttctggttc cagaagcatg agggactgaa gaaaatgaaa atgagtaaag
      181 atgaggtgaa acgagaagcc aaggatactg atggtaaccc tgaaattaaa ggggagcgcc
      241 gtcgccttca ttccgagata caaagtggaa gtttggctaa taacatcaaa aaatcaaccg
      301 ttattgttaa aaacccgact cacattgcga tttgcctata ctataaactt ggggagactc
      361 cattaccttt agttattgaa acaggaaaag atgccaaagc tctacagatc attaaactgg
      421 ctgaactcta tgatattcca gtgattgaag atattccttt agcaagaagt ctctataaga
      481 ata
//
LOCUS       DAZKIV010000461          457 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZKIV010000461 DAZKIV010000000
VERSION     DAZKIV010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.464"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>457)
                     /locus_tag="ACHOT6_005154"
     CDS             complement(<1..>457)
                     /locus_tag="ACHOT6_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFP9950146.1"
                     /translation="AVNRIPTVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPVGATGE
                     RGPGGDTGPAGPQGPKGDRGERGETGLTGSTGPQGPKGETGAAGPVGATGPQGPKGDP
                     GETQIRFRLGPASIIETNSNGWFPGTDGALITGLTFLDPKDATRVQGFFQ"
BASE COUNT           56 a          171 c          123 g          107 t
ORIGIN      
        1 gctgaaaaaa accctgaacc cgtgtggcat ctttggggtc aagaaaggtc agtccggtga
       61 tgagtgcgcc atctgtaccc gggaaccagc cattgctgtt tgtctcaata atgctcgccg
      121 gccccagacg aaaacggatt tgtgtctccc ccgggtcgcc cttcggtccc tgaggtccgg
      181 ttgcccccac cgggccagcc gcacctgttt ctcctttcgg tccctgtgga cctgtacttc
      241 ctgtcagccc ggtctctccc cgctctcccc tgtcgccttt cggcccctgc ggacctgccg
      301 gacctgtatc tcctcccggt ccccgttcgc cggttgcccc gacagggccg gtgtcaccac
      361 gctctccttt atctcccttc ggcccctgag gacccgcggg ccccggttct ccctttggtc
      421 cgggtggccc caccacggtg gggattcggt ttacggc
//
LOCUS       DAZKIV010000462          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZKIV010000462 DAZKIV010000000
VERSION     DAZKIV010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.465"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>455
                     /locus_tag="ACHOT6_005155"
     CDS             <1..>455
                     /locus_tag="ACHOT6_005155"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFP9950147.1"
                     /translation="IMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPE
                     NPIIGNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDS
                     NVIDELLRIKWWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDY"
BASE COUNT          151 a           60 c          100 g          144 t
ORIGIN      
        1 aataatgggt ggaaatcatc ctttgaatcg ttttactact cacatgatga cttataacgg
       61 tgaatttgat aagtttgcta tgagtgaatt tgaacgtagc tggacattga aaccattcat
      121 taccaaacct gaaaatccga ttataggaaa tgatgtctgg ataggaaatg atgttgtatt
      181 gaaaggtggc atcgcaatag gtgatggtgc tgttattgct gccaattccg ttgttaccaa
      241 agatgtgccc ccatatgcca tagttgcagg agttcctgca aaaataatca gatttaggtt
      301 tgattcaaat gttattgatg aactgttgag aataaaatgg tggaattata actactcaga
      361 tcttccggat aataacaagt gtgatgatat aaattatttt gttgaagaga tgaatcgatt
      421 gatatcagat ggaaatattc aagagaggga ctata
//
LOCUS       DAZKIV010000463          455 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZKIV010000463 DAZKIV010000000
VERSION     DAZKIV010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.466"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            90..299
                     /locus_tag="ACHOT6_005156"
     CDS             90..299
                     /locus_tag="ACHOT6_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950148.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(300..>455)
                     /locus_tag="ACHOT6_005157"
     CDS             complement(300..>455)
                     /locus_tag="ACHOT6_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950149.1"
                     /translation="RWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEH
                     GPLWIDW"
BASE COUNT          138 a          101 c           92 g          124 t
ORIGIN      
        1 taccggattc ttatccgtgt ccggcgcacg accacacgtg acagcgtgtt ggtctccatt
       61 tttaacccag aacctcaatg gaggataaaa tgccaaacaa aaaaagaaat ccgcttattg
      121 aaaaacagat tgaatgcctg gtaaatcaac tcaggcaatc agggttatta aaaactcatt
      181 cagagttgag gctcacagaa tcagcattcg acgataaatt aaataatgtc ctttataatg
      241 gcattattga ttttaatcgt tctgttggtc gccgcggccc tgctggtgtt tccttataat
      301 taccagtcaa tccagagtgg accgtgttca gcgtaaatat aactgtacac atccagatta
      361 tatttgtggt ctgttaagaa caggccgcaa atacatgccg aagcttccag tgcagcggct
      421 ctgttactga ataaccatgt agcaacattc cagcg
//
LOCUS       DAZKIV010000464          444 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZKIV010000464 DAZKIV010000000
VERSION     DAZKIV010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..444
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.467"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..>444
                     /locus_tag="ACHOT6_005158"
     CDS             183..>444
                     /locus_tag="ACHOT6_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFP9950150.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPAL"
BASE COUNT          107 a          110 c          111 g          116 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taatcctgcg ctgg
//
LOCUS       DAZKIV010000465          433 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZKIV010000465 DAZKIV010000000
VERSION     DAZKIV010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.468"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            240..>433
                     /locus_tag="ACHOT6_005159"
     CDS             240..>433
                     /locus_tag="ACHOT6_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950151.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVT"
BASE COUNT          133 a           78 c          123 g           99 t
ORIGIN      
        1 gtaaaaagaa taaaaaaatc ccgcagagtt gcggggacag acaaagatta acgttaagga
       61 gttatttttg tttctggtgc tcgggcaaaa aaaacattaa cgcagagaaa ttattagcac
      121 cacagtcagt ttgtgaaaat gtgaagatat tcagaagttt tattcagtca tgatacaggc
      181 atcctccggg atgcctgttg tttttgtgcg taacagttat cacagtaaag ggtgagacaa
      241 tgggcaaagg tggcggcaag gcgcacacgc cggttgaggc aaaggacaat cttaagtcca
      301 cgcagatgat gagcgtgatt gacgccattg gtgaagggcc gattgaaggt ccggtgaagg
      361 ggctgcagag tatcctggtg aacaaaaccc cgctgacgga cacggacggt aatcctgtga
      421 tacatggtgt gac
//
LOCUS       DAZKIV010000466          432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZKIV010000466 DAZKIV010000000
VERSION     DAZKIV010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.469"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            45..>432
                     /locus_tag="ACHOT6_005160"
     CDS             45..>432
                     /locus_tag="ACHOT6_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFP9950152.1"
                     /translation="MQVFSSDVYFTVGTNALLASQKEYYSDLVALVDLGHSFVVIDEH
                     QHRNLKPNTEPVNILLSNNFIRINKNITLSDLTHFLVSNLHTQNVYSTQEPLTHDEID
                     ILRLCVSYSLKQIAIIKGIDYKTISYH"
BASE COUNT          146 a           82 c           65 g          139 t
ORIGIN      
        1 cacgtaatat ttaccctaaa tgattttaga gtacaggtgg gcatatgcaa gtcttttcca
       61 gtgatgtcta cttcactgta ggcactaatg cattattagc atcccaaaaa gaatattaca
      121 gcgatttagt tgcgttggtt gatcttggac attcgttcgt tgttattgat gagcatcaac
      181 atcgaaatct caaaccaaat acagaacctg taaacatcct tttgagcaac aatttcatca
      241 ggatcaataa gaatattaca ttatctgatt taacgcattt cctggtttca aatcttcata
      301 cacaaaatgt gtattccaca caagaaccac ttacccatga tgagatagat atcctgcggt
      361 tatgtgtttc ttactcgtta aaacaaatag ccattattaa aggaattgat tataaaacaa
      421 tatcatatca ca
//
LOCUS       DAZKIV010000467          432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZKIV010000467 DAZKIV010000000
VERSION     DAZKIV010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.470"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>432)
                     /locus_tag="ACHOT6_005161"
     CDS             complement(<1..>432)
                     /locus_tag="ACHOT6_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9950153.1"
                     /translation="SGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINP
                     QNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHENMASSAYIHKNNK
                     LLYNLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRF"
BASE COUNT          137 a           92 c           64 g          139 t
ORIGIN      
        1 ctaaatctat cgcctgtatg atccccaagg aagatacaag gggttatcgc atcgtccttt
       61 aaaaaaacat gttgatgcaa ctcattcaat atcatgtctc tcgtatttaa taaattgtag
      121 agcaatttat tatttttatg tatgtatgca gatgaagcca tgttttcgtg ttttaataat
      181 tcacataata aactccaacc cttttccctg atgcctatat gccctgaaac gagcaatgca
      241 taaagtacag cgcctactct tccatctgta tcgccaaaat aagccgtgtt ggcattttgt
      301 gggttaatca caccacattg tttataaaag tctattttag aaaacacaga tttaacatag
      361 ctatcaaact tatccttagg aaacgtctct ttagagacat cccaacgcca gtcagcttta
      421 aaattaccgc ta
//
LOCUS       DAZKIV010000468          432 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZKIV010000468 DAZKIV010000000
VERSION     DAZKIV010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.471"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>432
                     /locus_tag="ACHOT6_005162"
     CDS             <1..>432
                     /locus_tag="ACHOT6_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFP9950154.1"
                     /translation="YVSTWQGFVYVAFIIDVFAGYIVGWRVSSSMETTFVLDALEQAL
                     WARRPSGTIHHSDKGSQYVSLAYTERLKEAGLLASTGSTGDSYDNAMAESINGLYKAE
                     VIHRKSWKNRAEVELATLTWVDWYNNRRLLGRLGHTPPAEAE"
BASE COUNT          108 a           99 c          132 g           93 t
ORIGIN      
        1 tacgtcagca catggcaggg cttcgtctat gtggcgttta tcattgatgt gtttgccgga
       61 tacatcgtgg ggtggcgggt ctcatcgtct atggaaacga cattcgtgct ggatgcgctg
      121 gagcaggcgt tgtgggcccg tcgtccgtct ggcaccatcc atcacagcga taaaggctct
      181 cagtatgtgt cactggccta tacggagcga ctaaaagaag ccggattact ggcatcaaca
      241 gggagtacag gcgactcgta tgacaacgcg atggctgaga gcatcaatgg tctttacaaa
      301 gcggaggtaa tacaccgtaa gagctggaaa aaccgtgcag aagtggaact ggccacacta
      361 acgtgggtgg actggtataa caatcgacga ttgctgggaa ggctgggcca tactcctccg
      421 gcagaagcag aa
//
LOCUS       DAZKIV010000469          429 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZKIV010000469 DAZKIV010000000
VERSION     DAZKIV010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.472"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..109)
                     /locus_tag="ACHOT6_005163"
     CDS             complement(<1..109)
                     /locus_tag="ACHOT6_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950155.1"
                     /translation="MSQAVDGTCIKAQQDKMVNSSLCKKASVDTYFNVME"
     gene            127..318
                     /gene="yfgG"
                     /locus_tag="ACHOT6_005164"
     CDS             127..318
                     /gene="yfgG"
                     /locus_tag="ACHOT6_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFP9950156.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT          111 a          112 c           76 g          130 t
ORIGIN      
        1 cttccataac attaaaatat gtatccactg acgctttttt acataacgaa gaattgacca
       61 ttttgtcctg ttgtgcctta atgcaagtac cgtccacagc ttgggacata cttcaaggaa
      121 ccttttgtga gtcaggcaac cagtatgcga aaacgacacc gatttaacag tcgcatgacc
      181 cgtatcgtac tgctcatcag ctttatcttc ttctttggcc gttttatcta ctcgtccgtc
      241 ggtgcctggc agcaccatca gagcaaaaaa gaagctcagc aatccacact ctccgtcgaa
      301 tcaccggtac aacgttagcg gttaccttct ccactttcac agaacataac ggcacttcgc
      361 tgtcggatgc ttttgctctt tggaattatc aaagcggcag atattctttc atcttaaatt
      421 ttacgtctt
//
LOCUS       DAZKIV010000470          427 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZKIV010000470 DAZKIV010000000
VERSION     DAZKIV010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.473"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>427
                     /locus_tag="ACHOT6_005165"
     CDS             <1..>427
                     /locus_tag="ACHOT6_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFP9950157.1"
                     /translation="YVRDFFVSPGEFRQTGTDASRTINEKQKPVIMFLVVGETARSQN
                     YALNGYSRGTNDFTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSE
                     NVLDILYRTGVNLLWIENDGGCKGVCKRIPTINIEPSNS"
BASE COUNT          139 a           71 c           94 g          123 t
ORIGIN      
        1 aatatgtaag ggattttttt gtttctcctg gcgagtttcg tcagactgga acggatgcat
       61 cccgcacaat aaatgaaaaa caaaagccgg taattatgtt tttagttgtc ggggagacag
      121 caagaagtca aaactatgct ctgaatggat attctcgtgg taccaatgat tttacaaaga
      181 aatataatga acttatatcc tttcataatg tgcagtcgtg tggcacatcc acagccatat
      241 ctgttccatg catgttttca gatatgaagc gaaaagagtt caattcccgc aaagcggtaa
      301 attcggaaaa tgtactggat attctgtaca gaacgggggt aaatttactc tggattgaaa
      361 atgacggggg atgtaaaggt gtatgtaagc gaatccctac aattaatatt gagccatcaa
      421 attcaga
//
LOCUS       DAZKIV010000471          424 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZKIV010000471 DAZKIV010000000
VERSION     DAZKIV010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.474"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>424)
                     /locus_tag="ACHOT6_005166"
     CDS             complement(<1..>424)
                     /locus_tag="ACHOT6_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9950158.1"
                     /translation="AEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGVVGGGV
                     TASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRL
                     MRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDG"
BASE COUNT           93 a          154 c           90 g           87 t
ORIGIN      
        1 taccgtcgtt ctgaatcacc acgtgattgt tctgattaaa attaatacct tccgcccgcc
       61 gcatctgcgc cggacttccg gcaccaccca cataaccacc ttccgcatag ccgcgcatca
      121 gacggtaaag attccccaca cctatccggc tggttgcctc tttcgtgaaa acaaactccc
      181 cgcggtgaac tatccccgca ggctcatatt tgccgccggt tcccgtaaat ccgccggtcg
      241 caaaatggaa gttcgctgcc gcagcctcaa tggccgtccc cgaggaagcc gttacaccac
      301 ctccaacaac cccgccaatg gcgctgccga tacgcccgac aatgcccacc atggcctgtt
      361 taagcaggat ttctgtcatc atggacagca ccgaacgggt gaatccccgc cagtctgcct
      421 ctgc
//
LOCUS       DAZKIV010000472          415 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZKIV010000472 DAZKIV010000000
VERSION     DAZKIV010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.475"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(20..>415)
                     /gene="ymfL"
                     /locus_tag="ACHOT6_005167"
     CDS             complement(20..>415)
                     /gene="ymfL"
                     /locus_tag="ACHOT6_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFP9950159.1"
                     /translation="DQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVEN
                     ADINQRLLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYK
                     IFCAPEKSDARECAAPGVVAFCVCGETNA"
BASE COUNT           97 a          118 c           94 g          106 t
ORIGIN      
        1 tagtgtgttg ttaaactgtt catgcgttag tttctccaca gacacaaaac gccacgacgc
       61 ccggagctgc acactcgcgg gcgtcactct tttctggagc gcagaagatt ttgtagacca
      121 gtgctgcatg ctcctggagc ttcgaaattg acagatacaa ctcatcatta attgctgtct
      181 gctcgtgtgg ctccacgacc ccatcttcga ttgccgaacg aatctgcttt gagtaattcc
      241 cgatctgttc gatgacttcc agcaggcgct ggtttatatc ggcgttctct acttcctcaa
      301 tttcaggaag cgatacaaac accccaccag cagactgtgc gacagcatcc gcaatgtagt
      361 gagtgccagc cgcgcgctgt aaaaccattg cccatcccag cgggaaaatc tgatc
//
LOCUS       DAZKIV010000473          412 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZKIV010000473 DAZKIV010000000
VERSION     DAZKIV010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.476"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            56..>412
                     /gene="ler"
                     /locus_tag="ACHOT6_005168"
     CDS             56..>412
                     /gene="ler"
                     /locus_tag="ACHOT6_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system LEE master regulator
                     Ler"
                     /protein_id="HFP9950160.1"
                     /translation="MNMETNSHTTSPYIQLIEQIAVLQQEAKRLREQEVQSVIESIQK
                     QITYYNITLQELGYTNVPDDGLARRNSSKGVYYRNEEGQTWSGVGRQPRWLKEALLNG
                     MKKEDFLVKDTEEEIIP"
BASE COUNT          146 a           71 c           98 g           97 t
ORIGIN      
        1 taaggataag gtcgctaata gcttaaaata ttaaagcatg cggagattat ttattatgaa
       61 tatggaaact aattcacata caacaagtcc atacattcag cttatagagc aaattgcagt
      121 tctacagcag gaagcaaagc gactgcgaga gcaggaagtt caaagtgtaa ttgagtcgat
      181 tcagaagcag attacttatt acaatataac cttacaagag ctgggatata ctaatgtgcc
      241 tgatgatgga ctcgctcgcc ggaactcatc gaaaggtgtt tactaccgca atgaagaagg
      301 gcagacctgg tcgggcgtag gccgacagcc acgctggctt aaagaagcac tgttgaatgg
      361 aatgaagaaa gaagattttc ttgtgaagga cactgaagaa gaaataatac cg
//
LOCUS       DAZKIV010000474          410 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZKIV010000474 DAZKIV010000000
VERSION     DAZKIV010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.477"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHOT6_005169"
     CDS             complement(<1..348)
                     /locus_tag="ACHOT6_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HFP9950161.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSL"
BASE COUNT          135 a           74 c           69 g          132 t
ORIGIN      
        1 caatgaatca aacttaaatt taacagcttc agttgctaat tgcattttac tttgaaacca
       61 ggagacaagt attgttgtga acatgcaaac cagcaaaaaa ggaatgttcg ttttcaacca
      121 accaaccccc atagctttaa aatagtccca aatatctggt ttagcgttat gatcaaggat
      181 gtattcaaga atcgacataa tgtgatgcac atcaaaaaca tatccgagat ataaagtacc
      241 cacaagcatt attacagaga tggttaagtc tctacttttt agaatttgcc cttttttaga
      301 agcatcctgg agtttctttt gtgttggctt ttcagtttta ttggccatga aacactctat
      361 ttctcaatgt atagttgcaa ttgttctggg aaaaaggata acggcattac
//
LOCUS       DAZKIV010000475          403 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZKIV010000475 DAZKIV010000000
VERSION     DAZKIV010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..403
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.478"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            19..95
                     /locus_tag="ACHOT6_005170"
     tRNA            19..95
                     /locus_tag="ACHOT6_005170"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:53..55,aa:Ile,seq:gat)
     gene            138..213
                     /locus_tag="ACHOT6_005171"
     tRNA            138..213
                     /locus_tag="ACHOT6_005171"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:171..173,aa:Ala,seq:tgc)
BASE COUNT          116 a           82 c           99 g          106 t
ORIGIN      
        1 gagcagtaaa acctctacag gcttgtagct caggtggtta gagcgcaccc ctgataaggg
       61 tgaggtcggt ggttcaagtc cactcaggcc taccaaattt gcatcgcaaa tttgaagagg
      121 ttttaactac atgttatggg gctatagctc agctgggaga gcgcctgctt tgcacgcagg
      181 aggtctgcgg ttcgatcccg catagctcca ccatctctgt agtgattaaa taaaaaatac
      241 ttcagagtgt acctgaaaag gttcactgcg aagttttgct ctttaaaaat ctggatcaag
      301 ctgaaaattg aaacactgaa caacgaaagt tgttcgtgag tctctcaaat tttcgcaaca
      361 cgatgatgaa tcgaaagaaa catcttcggg ttgtgaggtt aag
//
LOCUS       DAZKIV010000476          396 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZKIV010000476 DAZKIV010000000
VERSION     DAZKIV010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..396
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.479"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>396)
                     /gene="nleC"
                     /locus_tag="ACHOT6_005172"
     CDS             complement(<1..>396)
                     /gene="nleC"
                     /locus_tag="ACHOT6_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP9950162.1"
                     /translation="PSMTAVALTCNELYGYDVGQEPILPICEAGENENEEPYVSFSVA
                     PDTDSYEMPSWQEGLIHEIIHHVTGASDPSGDSNIELGPTEILARRVAQELGWSVPDF
                     KGYAEPEREAHLRLRNLNALRQAAMRHEK"
BASE COUNT           89 a          103 c           91 g          113 t
ORIGIN      
        1 tcttttcatg cctcatggca gcctgtcgaa gggcattcag gttacgtagt ctaagatgag
       61 cttctcgttc tggctctgca tatcctttga agtcgggaac actccatccc agctcttgag
      121 cgacacgacg tgcgagaatc tcggtgggtc ctagctctat attactatct ccagatggat
      181 cgctggcccc agtaacatga tgaataatct cgtgaatcag tccttcctgc cacgatggca
      241 tctcataaga atcagtatct ggcgcaacac taaaactgac ataaggctct tcgttctcgt
      301 tttctcctgc ctcgcaaatg gggagaattg gttcttgccc gacatcgtat ccatataatt
      361 cgttacaggt tagtgctacg gctgtcatgg acggcg
//
LOCUS       DAZKIV010000477          387 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZKIV010000477 DAZKIV010000000
VERSION     DAZKIV010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.480"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..>387
                     /locus_tag="ACHOT6_005173"
     CDS             2..>387
                     /locus_tag="ACHOT6_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950163.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKHRSKIVEID
                     KLDKHSQIDNDLGLHIIYFLQHGRWKVNEHSSQIEKIWFYNSELSIDIQEYNSFADNT
                     TDTFIFTIIPDNNHVIKLSSPITVKI"
BASE COUNT          148 a           65 c           58 g          116 t
ORIGIN      
        1 tatgaaagta tttcacaata tatttaagca cttctcttca aaccatcaag ataagcattc
       61 agataaagtt aacagtcatc aacaccatgg caaggttgat aaaaaacatc gttcaaagat
      121 agttgaaatt gataagctgg ataaacattc gcaaatagat aacgatttag gcctgcatat
      181 catctatttc ctgcaacatg gtcgctggaa ggtaaatgaa catagttctc agatagaaaa
      241 aatatggttt tacaatagtg agctatctat agatattcaa gaatataata gtttcgccga
      301 taatactact gacacgttta tctttacaat tataccagac aataaccatg tgataaaatt
      361 atcgtctccc attacagtta agattga
//
LOCUS       DAZKIV010000478          385 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZKIV010000478 DAZKIV010000000
VERSION     DAZKIV010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.481"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>385)
                     /locus_tag="ACHOT6_005174"
     CDS             complement(<1..>385)
                     /locus_tag="ACHOT6_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFP9950164.1"
                     /translation="GVSYEQLSRNYAQMSYSTARASANESWAYFMGRRKFVASRQASQ
                     MFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGSGRMAIDGLKEVQEAVMLI
                     EAGLSTYEKECAKRGDDYQEIFAQQV"
BASE COUNT           80 a          126 c           95 g           84 t
ORIGIN      
        1 acctgctggg caaaaatttc ctgatagtcg tcaccgcgtt tcgcgcactc tttctcgtag
       61 gtgctcagtc cggcttctat cagcatcacc gcttcctgaa cttctttcag accatcgatg
      121 gccatacgac cggagcctat ccagtcgcag ttcccccagg cactgcgggc ttcctgaaag
      181 ctgaagcgcg cttttgaagg taacgtcacc acgcggcgaa cgatggcctc ttccagccag
      241 cacagaaaca tctggctcgc ctgacgggat gcgacgaatt ttcgccgccc cataaagtac
      301 gcccacgact cgttcgcact ggcccgtgcc gtggagtagc tcatctgggc gtaattccgg
      361 gaaagctgct catacgagac accca
//
LOCUS       DAZKIV010000479          378 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZKIV010000479 DAZKIV010000000
VERSION     DAZKIV010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.482"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..362
                     /locus_tag="ACHOT6_005175"
     CDS             <1..362
                     /locus_tag="ACHOT6_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9950165.1"
                     /translation="LREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWVEIDNNIA
                     ENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWP
                     VNRVSELLPWRIALPAE"
BASE COUNT           89 a          100 c          112 g           77 t
ORIGIN      
        1 ggttgcgtga aaagatgaag accctgtcgc gacactcaga gttggcgaag gcgttcgcgt
       61 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
      121 acaacaacat cgctgaaaat gccctgcggg cggtcagtct gggtcgtaaa aacttcctgt
      181 tcttcggctc tgaccatggt ggtgagcggg gagcgctact gtacagcctg atcgggacgt
      241 gcaaactgaa tgacgtggat ccagaaagct accttcgcca tgtgcttggc gtcatagcag
      301 actggccggt caaccgggtc agcgaactgc ttccgtggcg catagcactg ccagctgaat
      361 aacacatccc cgtcaata
//
LOCUS       DAZKIV010000480          372 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZKIV010000480 DAZKIV010000000
VERSION     DAZKIV010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.483"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           88 a          102 c           66 g          116 t
ORIGIN      
        1 tccggcgtta gcttcactct ctggcaagca gccttactcc gctacctttg ggcttcgtgg
       61 ctggcccgtt ccgctcacac cgaaagcaaa gcgttacttt cttcgtgtaa ctatcgtggc
      121 ggtaacgccc acgaagaaat actattgcgc tgtgcgcaga agagtaatca cctaaccaat
      181 catactgatt gcttacgtgt taactctctc taactcttaa ttccacttcc tacatttaat
      241 aactctcgtt attctctgta gttgtgtaat tactcgaaga gagagcggct aaagctcaat
      301 tacactctgc tttcctcgtg taatttcact aagcctcaat acaattccac ttcctacatt
      361 ctgtagtcgt gt
//
LOCUS       DAZKIV010000481          371 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZKIV010000481 DAZKIV010000000
VERSION     DAZKIV010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.484"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>371
                     /locus_tag="ACHOT6_005176"
     CDS             <1..>371
                     /locus_tag="ACHOT6_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase zinc-finger binding
                     domain-containing protein"
                     /protein_id="HFP9950166.1"
                     /translation="RMQFGKSSEKLRAKTERQIQEAQELISALQEEMAETLGEQYDPV
                     LPSPLRQSSARKPLPASLPRETQVIRPEEECCPACSGELSPLGCDVSEQLELISSAFK
                     VIETQRPKQACCRCDHIVQAPV"
BASE COUNT           99 a          102 c          100 g           70 t
ORIGIN      
        1 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagatacag
       61 gaagcacagg agctaatcag cgcacttcag gaagaaatgg cagaaacgct gggtgagcaa
      121 tatgacccgg tactgccatc ccccctgcgc cagtcttcag cccgtaaacc gttaccggct
      181 tcacttcccc gtgaaaccca ggtcatccgg ccggaagagg aatgctgtcc ggcctgtagt
      241 ggtgaactca gtccactggg atgtgatgtg tcagagcaac tggagcttat cagcagtgcc
      301 tttaaggtta tcgaaacaca acgtccgaaa caggcctgtt gccggtgcga ccatatcgtg
      361 caggcaccgg t
//
LOCUS       DAZKIV010000482          367 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZKIV010000482 DAZKIV010000000
VERSION     DAZKIV010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.485"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..198)
                     /locus_tag="ACHOT6_005177"
     CDS             complement(<1..198)
                     /locus_tag="ACHOT6_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFP9950167.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNAT"
     gene            206..>367
                     /locus_tag="ACHOT6_005178"
     CDS             206..>367
                     /locus_tag="ACHOT6_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFP9950168.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRTYFSNVCA"
BASE COUNT           95 a           89 c           79 g          104 t
ORIGIN      
        1 agtggcattt ttccactcaa ccagccagga agccattttc ccgtaaggga tgtatttatt
       61 accggcctca tgattctcat tctgacgtgc cagagcacga ttgaaaacga aacgacatgc
      121 gcctgcgaag cgcctcattt cacgctcctg ttgaccaccg ggtcttaact ggaatttaaa
      181 tgcttgtagt cgtttcacag gccaaatgat actttggtct gtgaaaaaag aaaccgatat
      241 ccggcgtgga aggcattgtg ttttcctgat gcatgttcac ctggtctttg tcaccagata
      301 ccgacgtcag atttttgacc atgacgcgac agaaaaacta cgcacttact tttcaaatgt
      361 atgtgct
//
LOCUS       DAZKIV010000483          364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZKIV010000483 DAZKIV010000000
VERSION     DAZKIV010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.486"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..363
                     /locus_tag="ACHOT6_005179"
     CDS             <1..363
                     /locus_tag="ACHOT6_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
                     /protein_id="HFP9950169.1"
                     /translation="YYGNVASEKLLIPTQDTRDIIAAAVRILDRIWVDGHRYAKAGCI
                     LNDFTPTGVSHLNLFDDTQPRSNSNQPLKVVDSINHSGLGKVWFAGRGIAPEWQIKRE
                     MSSPAYTTRWNELPVARL"
BASE COUNT           95 a           90 c           98 g           81 t
ORIGIN      
        1 tattacggga atgtagccag tgaaaaactg ctcatcccca cgcaggatac acgggatatc
       61 attgccgctg ccgttagaat tctggacagg atatgggtgg atggccatcg atatgcaaaa
      121 gcgggttgca tactcaacga tttcactcct accggggttt cgcatttgaa ccttttcgat
      181 gatactcagc cccggagcaa cagtaaccag ccgttgaaag tcgtggacag cattaaccat
      241 tccggtctgg ggaaagtgtg gtttgcaggc cgaggcattg cgccagaatg gcagataaag
      301 cgtgagatgt cgtcaccggc atacactacc cgctggaacg aactaccggt cgccagactt
      361 tgaa
//
LOCUS       DAZKIV010000484          364 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZKIV010000484 DAZKIV010000000
VERSION     DAZKIV010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.487"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>364
                     /locus_tag="ACHOT6_005180"
     CDS             <1..>364
                     /locus_tag="ACHOT6_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HFP9950170.1"
                     /translation="FILCQHLIANTEFIYLALPENYNRLFLPNSKNQTNNRFKTLNSK
                     AIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEKTAGIAIATASILLTKRS
                     NVDTYTEINSYLGKLRGQQ"
BASE COUNT          136 a           66 c           65 g           97 t
ORIGIN      
        1 tttatcctct gtcaacatct tatcgcaaat accgagttca tctatttggc actccctgaa
       61 aattataacc gtttgttttt accaaattca aaaaaccaaa caaataatcg tttcaaaacg
      121 ttaaacagca aagctattgg caggctactt gctgctggtg gcgtatacaa tgggaacatt
      181 gaaggattca gagatactgc ggaaaaactg ggtggagatg caataaaagg ctatgatcaa
      241 atactaaatg aaaaaacagc gggcatagcg atagcaacag catctattct tttaacaaag
      301 cgttctaatg ttgatacata tacagaaata aatagttact taggcaaact tagaggtcaa
      361 caaa
//
LOCUS       DAZKIV010000485          363 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZKIV010000485 DAZKIV010000000
VERSION     DAZKIV010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.488"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>363)
                     /locus_tag="ACHOT6_005181"
     CDS             complement(<1..>363)
                     /locus_tag="ACHOT6_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9950171.1"
                     /translation="QNWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGASA
                     STGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMR
                     GYAEGGYVGGAGSPAQMRR"
BASE COUNT           80 a          138 c           84 g           61 t
ORIGIN      
        1 ccgccgcatc tgcgccggac ttccggcacc gcccacataa ccaccttccg catagccccg
       61 catcaggcgg tacaggttgc cgacgccaat ccggctggtt gcttccttcg tgaagacaaa
      121 ctccccgcga tgaacaattc ccgcaggttc atatttaccc cccgtccccg taaatccccc
      181 ggtcgcgaaa tggaagttcg ccgccgcagc ctgaatggcc gtccccgtgg aggcagacgc
      241 accaccaccg aaagcaccac ccatggcgct gccgatactc ccgactatcc ccaccatcgc
      301 ctgcttcaga aaaatctctg tcagcatgga gagcacagaa cgggtgaaac cacgccagtt
      361 ctg
//
LOCUS       DAZKIV010000486          360 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZKIV010000486 DAZKIV010000000
VERSION     DAZKIV010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.489"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..160
                     /locus_tag="ACHOT6_005182"
     tRNA            85..160
                     /locus_tag="ACHOT6_005182"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:119..121,aa:Glu,seq:ttc)
BASE COUNT           99 a           78 c           87 g           96 t
ORIGIN      
        1 cttaaagaag cgttctttgc agtgctcaca cagattgtct gatagaaagt gaaaagcaag
       61 gcgtcttgcg aagcagactg atacgtcccc ttcgtctaga ggcccaggac accgcccttt
      121 cacggcggta acaggggttc gaatccccta ggggacgcca cttgctggtt tgtgagtgaa
      181 agtcacctgc cttaatatct caaaactcat cttcgggtga tgtttgagat atttgctctt
      241 taaaaatctg gatcaagctg aaaattgaaa cactgaacaa cgaaagttgt tcgtgagtct
      301 ctcaaatttt cgcaacacga tgatggttcg caagaaacat cttcgggttg tgaggttaag
//
LOCUS       DAZKIV010000487          348 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZKIV010000487 DAZKIV010000000
VERSION     DAZKIV010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.490"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..348
                     /gene="tccP"
                     /locus_tag="ACHOT6_005183"
                     /gene_synonym="espF(U)"
                     /pseudo
     CDS             17..348
                     /gene="tccP"
                     /locus_tag="ACHOT6_005183"
                     /gene_synonym="espF(U)"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310742.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP"
BASE COUNT          106 a           97 c           52 g           93 t
ORIGIN      
        1 ttataaggta atctgcatga ttaacaatgt ttcttcactt tttccaaccg tcaaccgcaa
       61 tattacagct gtatataaaa aagcagcttc tctgtatcac cacagaaaat cacattaaat
      121 cctgtaaaaa tcagctcacc tttttcacca agcagtagct ccatcagcgc aacaactctc
      181 tttcgagccc caaacgccca ttcggcatca tttcatcgac agtctactgc tgaaagttcg
      241 ttacatcaac aacttcctaa tgtgaggcag cgcctgatac aacatcttgc agagcatggc
      301 attaaacctg cccggagtat ggctgaacat attcctccgg cacctaac
//
LOCUS       DAZKIV010000488          347 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZKIV010000488 DAZKIV010000000
VERSION     DAZKIV010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.491"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>347
                     /locus_tag="ACHOT6_005184"
     CDS             <1..>347
                     /locus_tag="ACHOT6_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFP9950172.1"
                     /translation="TPSGPSADTSVRTISLLPAAGEAAAQGWSIKDGGIQLSDGVFKI
                     TKQSNKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTNNQFGLGIYLYTDAPVPDGV
                     AMTGTGNPFLMSY"
BASE COUNT           73 a           92 c          102 g           80 t
ORIGIN      
        1 acgccatcgg gtccgtctgc agatacgtcc gttcgcacaa tctccctgct gccggcagcc
       61 ggagaggctg ctgcgcaggg ctggagcatt aaggatggcg gaattcagtt gtcagatggt
      121 gtatttaaga tcaccaagca gagcaataaa acctggtccc tgacgcatcc ggtggatgac
      181 gcaattaccc tgctgacaca gggcggcaga ctgacctgta agttccgcct gtcaggcgca
      241 ctgaccaaca atcagttcgg gctggggatt tatctgtata cggatgctcc cgttcctgat
      301 ggtgtggcga tgacgggtac cggtaatccg ttcctgatgt cgtactt
//
LOCUS       DAZKIV010000489          343 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZKIV010000489 DAZKIV010000000
VERSION     DAZKIV010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.492"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..77
                     /locus_tag="ACHOT6_005185"
                     /pseudo
     CDS             1..77
                     /locus_tag="ACHOT6_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          102 a           93 c           64 g           84 t
ORIGIN      
        1 ctggctattt tgccagtagc tgcggaggtg cgccaatatc cgtcatccgc caatacattg
       61 aacaacagca aacacctggt caggtggaaa accgcgcctt atatccctgc cctgaaggac
      121 ggggttttac ggcgcaccgg ataagcctgg agattatcta caccagcatg gacatcattg
      181 tactatgccc cgtacatgcc ccatcacatc ctcaacatca aagtattaga gccttgcaaa
      241 ccattatgcg tctcagtttg tctcatctat cttacgcaaa aaaaatagaa ctaatatcaa
      301 gcggcgctat attgcgtatt aaataaaacc acctcaatta cat
//
LOCUS       DAZKIV010000490          342 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZKIV010000490 DAZKIV010000000
VERSION     DAZKIV010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.493"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>342)
                     /locus_tag="ACHOT6_005186"
     CDS             complement(<1..>342)
                     /locus_tag="ACHOT6_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9950173.1"
                     /translation="LNGISNAVSTIGRHLTGVASRVGSAGVSGFSVAPQTVRLTPVRF
                     HSPFSPGSSNINARTTFNMSHMSQMSRMSQMATSFTPTRPAPPPPTSGQASGSAGLPP
                     IAQALKDHLAA"
BASE COUNT           96 a           85 c           86 g           75 t
ORIGIN      
        1 aggcagctaa atgatctttt aatgcctgtg caatgggcgg tagcccggca gacccggatg
       61 cctgtccact tgtcggtggc ggtggtgccg ggcgagtagg agtaaatgaa gtcgccatct
      121 gactcatacg actcatctga ctcatatgac tcatattaaa agtcgttctc gcattaatat
      181 tcgacgaacc tggagaaaaa ggggaatgaa atctcaccgg agtaagacgc acagtctgag
      241 gggctacaga aaatccagat acccccgcag agcccactcg acttgcgaca cctgtaagat
      301 gccgccctat tgtagaaaca gcgttactaa ttccattaag ca
//
LOCUS       DAZKIV010000491          338 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZKIV010000491 DAZKIV010000000
VERSION     DAZKIV010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.494"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..264)
                     /locus_tag="ACHOT6_005187"
     CDS             complement(<1..264)
                     /locus_tag="ACHOT6_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tir chaperone family protein"
                     /protein_id="HFP9950174.1"
                     /translation="MNEQFCKDLAHTFGITLEEQTDALAFHDNDGHEWILECAPQSEI
                     LFFYCYLSTNEPDQINKILEINNNREMLGMFFLSLKDDKILLNI"
BASE COUNT           99 a           53 c           61 g          125 t
ORIGIN      
        1 gatgtttaag agaattttat cgtcttttaa tgataagaaa aacataccaa gcatttctcg
       61 gttgttgttt atttctaata ttttattgat ttgatctggt tcgttggttg ataggtaaca
      121 ataaaaaaac aaaatttctg attgaggtgc gcactcaagt atccactcat gaccatcgtt
      181 atcgtgaaaa gcgagggcgt cagtttgctc ttccaatgtt atcccaaacg tatgtgcaag
      241 atctttgcaa aattgttcat tcatattgta gtcctgtcat tcttataacg ctgatgtgtt
      301 cataggtaat ttatttgtga gttcttgcac aaataaca
//
LOCUS       DAZKIV010000492          333 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZKIV010000492 DAZKIV010000000
VERSION     DAZKIV010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.495"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            22..288
                     /gene="ariR"
                     /locus_tag="ACHOT6_005188"
                     /gene_synonym="ymgB"
     CDS             22..288
                     /gene="ariR"
                     /locus_tag="ACHOT6_005188"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFP9950175.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLS
                     GDNVNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT          119 a           49 c           63 g          102 t
ORIGIN      
        1 taagtaagat gagaggttac catgcttgaa gatactacaa ttcataatgc aataactgat
       61 aaagcgttag caagttactt tcgcagttcg ggtaatttgt tagaagaaga gtcagcagtg
      121 ttagggcagg ctgtcaccaa tttaatgctt tcaggcgata atgttaataa taaaaatatt
      181 atcttaagtc tgatacactc cctggaaaca acaagtgata ttctcaaagc tgatgtgatt
      241 agaaaaacac tggaaatcgt gttgcgatac acagctgatg atatgtaact tctaattatt
      301 aagtataagt ttatagaaaa ctcattcatc tta
//
LOCUS       DAZKIV010000493          330 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZKIV010000493 DAZKIV010000000
VERSION     DAZKIV010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.496"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          101 a           64 c           65 g          100 t
ORIGIN      
        1 aataatattt attaaaattc caggtcttct ttttctgatt acagaaggca agtggtaatt
       61 acgtatagtt tcccgataaa gacacgacag caacatcccg cataaggcat tgttctcttt
      121 atctttgtac ggtacttcat ggaacagaga ttttgacctt gcgaatcgtg atgtctgttg
      181 gggagggcaa tttgctcact gaagcgtgag gctcgattaa gcgcacgaaa cacagatatc
      241 aaaaaacccg gtcgctttct acaaggtaac cgggtaaaaa taatttttat tttttaactg
      301 ctctgagact cataaagatg cctcaaaact
//
LOCUS       DAZKIV010000494          327 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZKIV010000494 DAZKIV010000000
VERSION     DAZKIV010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.497"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            24..317
                     /locus_tag="ACHOT6_005189"
     CDS             24..317
                     /locus_tag="ACHOT6_005189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFP9950176.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
BASE COUNT          120 a           57 c           62 g           88 t
ORIGIN      
        1 atcttgcgta agagaaagtt gatatgtcat tagtaggata taatagtaac atcataataa
       61 ccatcgcgct gatatcgttg ctctctgcat gcaccagaga aaattcatta aaggagaatc
      121 aaaataaaag aagtggcgaa tcccaagcga tagtagatgc aagacagttc tttagcgaac
      181 acccggaata tatatcatca gcagaactgg agcaaaagct atatcgcgaa tttgcacagg
      241 ttactacagt tccagcttat aaagagatga gtatgtatca attgcttatc atttcacaag
      301 atcgtttaac tcattaaata aggatct
//
LOCUS       DAZKIV010000495          325 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZKIV010000495 DAZKIV010000000
VERSION     DAZKIV010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.498"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..167)
                     /gene="tnpB"
                     /locus_tag="ACHOT6_005190"
     CDS             complement(<1..167)
                     /gene="tnpB"
                     /locus_tag="ACHOT6_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536669.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFP9950177.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKL"
BASE COUNT           67 a           90 c           90 g           78 t
ORIGIN      
        1 agtttaacct gactgccgct gcggccccgg aaaatgaaaa catggccgga catgggatcg
       61 tctttcagcg ccgtctgtac tttcgcagcc aggccgttga agccatttct catatcggtg
      121 ataccggcaa ccagccaaat tttggtcccg gaaggtaacg ggatcatcgc ttcagttcct
      181 gtatcagcag agtcaggagc ttttcgctga cattgccatt gaagcggagc gtcccgtgcc
      241 ggaacgttac ctcacagctg atactgaggg tttccgggtc ctctgcgagc gattctggct
      301 gttcggcagc tgcatcgaga gtcac
//
LOCUS       DAZKIV010000496          318 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZKIV010000496 DAZKIV010000000
VERSION     DAZKIV010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.499"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /gene="essD"
                     /locus_tag="ACHOT6_005191"
     CDS             <1..99
                     /gene="essD"
                     /locus_tag="ACHOT6_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFP9950178.1"
                     /translation="GVLGSLLFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            99..>318
                     /locus_tag="ACHOT6_005192"
     CDS             99..>318
                     /locus_tag="ACHOT6_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFP9950179.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKA"
BASE COUNT           83 a           65 c           91 g           79 t
ORIGIN      
        1 ggtgtgctgg gaagtctgct gtttggcctg ctgacgtatc tgacaaatct ttatttcaag
       61 attaaagaag ataagcgtaa ggctgcgaga ggtgaataat gtcgccgtca ttacgcaagg
      121 ctgttgcagc tgctattggt ggtggggctg ttgccatagc gtctgtgctc atcactggtc
      181 caggtggtaa cgatggtctg gaaggtgtca gctacatacc atacaaagat atcgttggcg
      241 tatggactgt atgtcacgga cacaccggaa aagacatcat gcccggtaaa acgtataccg
      301 aagcagaatg caaagccc
//
LOCUS       DAZKIV010000497          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZKIV010000497 DAZKIV010000000
VERSION     DAZKIV010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.500"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..296)
                     /locus_tag="ACHOT6_005193"
     CDS             complement(<1..296)
                     /locus_tag="ACHOT6_005193"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950180.1"
                     /translation="MGRLPRIYYGLVCRESLDHVFNLTGQYFPGPSPDMANAASLSLV
                     IKSFYYLNFPVFIAGSSSKSAAGLGLKRKHLGAISEIKHLPMNTMAEWNKNIPD"
BASE COUNT           97 a           64 c           60 g           93 t
ORIGIN      
        1 aaatcgggta tatttttatt ccactcagcc attgtattca taggtaggtg tttaatttcg
       61 gatatagcac caagatgctt acgttttaac cccaaaccgg ctgcactttt tgaggatgaa
      121 ccagcaataa aaactggaaa atttaaataa tagaaactct ttataactaa actcaaactt
      181 gctgcattag ccatatcagg gcttggtccg gggaaatatt gtcctgttag attaaataca
      241 tggtcaaggg attccctaca gacaaggcca taatatatgc gtggtaacct tcccatactg
      301 gtacctccgt ggcg
//
LOCUS       DAZKIV010000498          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZKIV010000498 DAZKIV010000000
VERSION     DAZKIV010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.501"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>314
                     /locus_tag="ACHOT6_005194"
     CDS             <1..>314
                     /locus_tag="ACHOT6_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFP9950181.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VY"
BASE COUNT           80 a           70 c           88 g           76 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaaag agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acat
//
LOCUS       DAZKIV010000499          314 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZKIV010000499 DAZKIV010000000
VERSION     DAZKIV010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.502"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..269)
                     /locus_tag="ACHOT6_005195"
     CDS             complement(<1..269)
                     /locus_tag="ACHOT6_005195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFP9950182.1"
                     /translation="MHYGKVKIFDINHSIVSQYLEIQHKLTRTHLTDVPLYLSLEPNN
                     PALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGCLS"
BASE COUNT           98 a           64 c           67 g           85 t
ORIGIN      
        1 ctggataaac atccacttaa aaataataat atccgttcat ctgattcaaa cagcgacagg
       61 catgccatca taagaaacat atccgtggta tctccggaaa atctctggct ggtaattaaa
      121 gcctcagcca acgcagggtt gttgggttcc agtgacagat aaagcggaac gtcagtcaga
      181 tgagttcttg tcagcttatg ctgaatttcc agatattgac ttactatgga atggtttata
      241 tcgaaaattt taactttgcc ataatgcata aggaaaagcg ccctgatgca tttggtggcc
      301 agaacgactg agcc
//
LOCUS       DAZKIV010000500          310 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZKIV010000500 DAZKIV010000000
VERSION     DAZKIV010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.503"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>310)
                     /locus_tag="ACHOT6_005196"
     CDS             complement(<1..>310)
                     /locus_tag="ACHOT6_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFP9950183.1"
                     /translation="DVAVTESYKPIWNWKISQYNVFDIKFETMIKPQFADRTYFSNCS
                     PVDPTSTRPTYFGDTDGSVGAVLYALFATGHLGIMAEGENFLSQLLNIEDEVLNVLLR
                     E"
BASE COUNT           88 a           67 c           63 g           92 t
ORIGIN      
        1 tctcccttag caatacgttg agaacttcat cttctatatt aagcaactgg ctaaggaaat
       61 tttctccttc agccatgatt cccaaatgtc ccgttgcaaa aagcgcgtac aaaactgcac
      121 cgactgagcc atcggtatcg ccaaaatagg tcggcctggt acttgtggga tcaacaggtg
      181 aacaattgga gaaataggtt ctgtcagcga actggggttt tatcattgtt tcgaatttaa
      241 tatcaaatac attgtattgc gatattttcc agttccagat gggtttgtac gactcagtaa
      301 ctgcaacatc
//
LOCUS       DAZKIV010000501          305 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZKIV010000501 DAZKIV010000000
VERSION     DAZKIV010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.504"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>305
                     /locus_tag="ACHOT6_005197"
     CDS             <1..>305
                     /locus_tag="ACHOT6_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFP9950184.1"
                     /translation="QEDRAHAALLALETELRTLEKHSGANEKISRQRRDLWKAESQYA
                     VLKEAATKRQLSEQEKSLLAHKDETLEYKRQLAELGDKVEYQKRLNELAQQAVRFEE"
BASE COUNT           89 a           71 c           99 g           46 t
ORIGIN      
        1 caggaagacc gggcacatgc tgccctgctg gcgcttgaaa ctgagctcag gacgctggaa
       61 aaacacagcg gtgcgaatga gaaaatcagc cggcagcgcc gtgatttatg gaaggcggaa
      121 agtcagtatg cggtcctgaa agaggctgcc acgaaacgac agttatctga gcaggaaaaa
      181 tccctgctgg cgcataaaga cgagacgctg gagtacaaac gccagctggc tgagctgggc
      241 gacaaggttg aataccagaa acgcctgaat gagctggcac agcaggcggt gcggtttgaa
      301 gagca
//
LOCUS       DAZKIV010000502          303 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZKIV010000502 DAZKIV010000000
VERSION     DAZKIV010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.505"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..>303
                     /locus_tag="ACHOT6_005198"
     CDS             38..>303
                     /locus_tag="ACHOT6_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFP9950185.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITG"
BASE COUNT           84 a           59 c           90 g           70 t
ORIGIN      
        1 tcgtgagtaa aaagattcgg tatcagaaga ggtgagtatg gctaacgcct ggctcagatt
       61 atggcatgac atgccaaatg accctaagtg gcgaacaatt gccagggtgt cagggcagcc
      121 aattgcaaca gtgatggcag tgtatatcca cctcctggtg agcgcgtcac gaaatgtcac
      181 gcgaggtcac attgatgtca cgacagaaga tttggcaagt gcgctcgacg tgacagaaga
      241 ggtaattgat tcaattttgc agacgatgca ggggcgggta cttgatggtg atttaatcac
      301 tgg
//
LOCUS       DAZKIV010000503          279 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZKIV010000503 DAZKIV010000000
VERSION     DAZKIV010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.507"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>279
                     /locus_tag="ACHOT6_005199"
     CDS             <1..>279
                     /locus_tag="ACHOT6_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFP9950186.1"
                     /translation="EPLVESRYLYDPLGRRVAKRVWRRERDLTGWMSLSRKPQVTWYG
                     WDGDRLTTIQNDRSRIQTIYQPGSFTPLIRVETATGELARTQRRSLADA"
BASE COUNT           63 a           81 c           94 g           41 t
ORIGIN      
        1 agagccgctg gtcgaaagtc gctatcttta cgacccgctg ggccgcaggg tggcaaaacg
       61 ggtgtggcgg cgtgaacggg acctgacggg ctggatgtcg ctgtcacgga aaccgcaagt
      121 gacctggtac ggctgggacg gcgaccggct gaccacgata cagaacgaca ggagccgcat
      181 ccagacgatt tatcagccgg ggagcttcac accactcatc agagttgaaa ctgccaccgg
      241 tgaactggcc agaacgcagc gccgcagcct ggcggatgc
//
LOCUS       DAZKIV010000504          269 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZKIV010000504 DAZKIV010000000
VERSION     DAZKIV010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.508"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..258)
                     /locus_tag="ACHOT6_005200"
     CDS             complement(37..258)
                     /locus_tag="ACHOT6_005200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFP9950187.1"
                     /translation="MKIIPRGEAMRIHQQHPASRLFPFCTGKYRWHGSAETYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
BASE COUNT           67 a           74 c           72 g           56 t
ORIGIN      
        1 gtggcagcga aaattcggct catcatttct cctgactcag ttcagagtga cgctcatcag
       61 ccggacatac gggccaaaac tgtccttacg gcgttcggca aacacagcca gcacgccggg
      121 aatatcctgc acttcacgac cggtatacgt ctcagcactg ccgtgccagc ggtacttacc
      181 ggtgcagaac ggaaaaagac gggatgccgg atgctgttgg tgaatacgca tggcttcacc
      241 acgggggata attttcataa tgggatacc
//
LOCUS       DAZKIV010000505          268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZKIV010000505 DAZKIV010000000
VERSION     DAZKIV010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.509"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>268)
                     /gene="sepL"
                     /locus_tag="ACHOT6_005201"
     CDS             complement(<1..>268)
                     /gene="sepL"
                     /locus_tag="ACHOT6_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system LEE gatekeeper SepL"
                     /protein_id="HFP9950188.1"
                     /translation="LSWLGVGELNQEGIQKIKKLYEKAKDEDSENGASLLDWFMEVKD
                     LPEREKHLKVIIRALSFDLSYMSSFEDKVKTSSIISDLCRIIVF"
BASE COUNT           86 a           66 c           41 g           75 t
ORIGIN      
        1 aaaaaaacga ttatccggca taaatcgcta ataattgaag atgtttttac cttgtcttca
       61 aaagaagaca tataagaaag atcgaacgat agcgccctaa taataacctt taagtgcttc
      121 tcacgctctg gaagatcctt gacctccata aaccagtcaa gtaaagaggc tccgttttca
      181 gagtcctcgt ccttagcctt ttcataaagc ttcttgatct tctggatacc ttcctgattt
      241 aactcaccca cccctaacca ggaaagca
//
LOCUS       DAZKIV010000506          268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZKIV010000506 DAZKIV010000000
VERSION     DAZKIV010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.510"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..76
                     /locus_tag="ACHOT6_005202"
     CDS             <1..76
                     /locus_tag="ACHOT6_005202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_044783345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system LEE effector EspF"
                     /protein_id="HFP9950189.1"
                     /translation="SGGLPPIAQALKDHLAAYEQSKKV"
BASE COUNT           75 a           63 c           50 g           80 t
ORIGIN      
        1 gtctggtgga ctaccgccca ttgcacaggc attaaaagat catttagctg cctatgagca
       61 atcgaaaaaa gtataatcaa ctaatttaaa acgattaacc ggtgttgatt atttatttgc
      121 caactaataa aagccgatat caaactgtgt agtaatatac tcgtcatact tcaagttgca
      181 tgtgttgcga ctgcgttcgc tccccccagt cacttactta tgtaagctcc tgggggttca
      241 ctcgcttgcc gccttcctgc aattcgaa
//
LOCUS       DAZKIV010000507          268 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZKIV010000507 DAZKIV010000000
VERSION     DAZKIV010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.511"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..148)
                     /gene="rrf"
                     /locus_tag="ACHOT6_005203"
     rRNA            complement(33..148)
                     /gene="rrf"
                     /locus_tag="ACHOT6_005203"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(188..263)
                     /locus_tag="ACHOT6_005204"
     tRNA            complement(188..263)
                     /locus_tag="ACHOT6_005204"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Thr,seq:ggt)
BASE COUNT           56 a           80 c           63 g           69 t
ORIGIN      
        1 gtcttccgac tgagcctttt gttttatttg atgtctggca gttccctact ctcgcatggg
       61 gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag
      121 gtgggaccac cgcgctactg ccgccagaca aattcttttc taatctgccg aactttaacc
      181 taaaaagtgg tgctgatacc cagagtcgaa ctggggacct cacccttacc aagggtgcgc
      241 tctaccaact gagccatatc agcacgct
//
LOCUS       DAZKIV010000508          267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZKIV010000508 DAZKIV010000000
VERSION     DAZKIV010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.512"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>267
                     /locus_tag="ACHOT6_005205"
     CDS             <1..>267
                     /locus_tag="ACHOT6_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0046903 - secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HrpJ domain-containing protein"
                     /protein_id="HFP9950190.1"
                     /translation="NELAMITSSSLSETIEGLSLGYRKGSARKEEEGTTTEKLLNDMQ
                     ELLTLTDSDKLKELSLKNSGLLEQHDPNLAMFGNMPKGEIVALI"
BASE COUNT           90 a           48 c           69 g           60 t
ORIGIN      
        1 aaaatgaatt agcgatgatt accagttcgt cgctttctga aacgattgaa gggctaagcc
       61 tagggtatcg caaaggtagc gccaggaaag aagaggaagg tacgacaacg gagaagctgc
      121 tcaatgatat gcaagagctg cttactctga ccgatagcga taaactaaaa gaactatcat
      181 taaagaacag tgggctgctg gaacagcatg atcctaactt ggctatgttt ggtaatatgc
      241 caaaagggga aattgtcgcg ctcatat
//
LOCUS       DAZKIV010000509          267 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZKIV010000509 DAZKIV010000000
VERSION     DAZKIV010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.denovo.513"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>267
                     /gene="nleC"
                     /locus_tag="ACHOT6_005206"
     CDS             <1..>267
                     /gene="nleC"
                     /locus_tag="ACHOT6_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
                     /protein_id="HFP9950191.1"
                     /translation="KRIPLSAADLSNVYESVIRAVHDSRSRLIDQHTVDMIGNTVLDA
                     LSRSQTFRDAVSYGIHNEEVHIGCIKYRNEYELNGESAIKIDDIQ"
BASE COUNT           85 a           56 c           60 g           66 t
ORIGIN      
        1 aagcgaatac cgctttccgc agcagattta agcaacgtat acgaaagtgt aattcgcgcc
       61 gtccatgaca gccgtagcag gctcatcgat cagcatacgg tcgatatgat tggcaacact
      121 gtacttgatg cgttgagccg atcacaaaca tttcgtgatg ccgtaagcta tggcattcat
      181 aatgaggagg tacacattgg ttgcattaaa tacagaaacg aatacgagct taacggagaa
      241 tctgctatca aaattgatga tattcaa
//
LOCUS       DAZKIV010000510         7170 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.guided.190, whole genome shotgun sequence.
ACCESSION   DAZKIV010000510 DAZKIV010000000
VERSION     DAZKIV010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7170
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.guided.190"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1218
                     /locus_tag="ACHOT6_005207"
     CDS             <1..1218
                     /locus_tag="ACHOT6_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFP9950192.1"
                     /translation="RVYRYNTNGGIVTGDGMGMALSHGVPLRDMEFVQYHPTGLPGSG
                     ILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLGEPKNKYMELGPRDKVSQAFWHEW
                     RKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICELAKAYVGVDPVKEPIPVRPTAHY
                     TMGGIETDQNCETRIKGLFAVGECSSVGLHGANRLGSNSLAELVVFGRLAGEQATERA
                     ATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWAKIRDEMGLAMEEGCGIYRTPELM
                     QKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIELGHGLNVAECMAHSAMARKESRG
                     AHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEYSDVKITTLPPAKRVYGGEADAAD
                     KAEAANKKEKANG"
     gene            1211..1945
                     /gene="frdB"
                     /locus_tag="ACHOT6_005208"
     CDS             1211..1945
                     /gene="frdB"
                     /locus_tag="ACHOT6_005208"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFP9950193.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            1956..2351
                     /gene="frdC"
                     /locus_tag="ACHOT6_005209"
     CDS             1956..2351
                     /gene="frdC"
                     /locus_tag="ACHOT6_005209"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFP9950194.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            2362..2721
                     /gene="frdD"
                     /locus_tag="ACHOT6_005210"
     CDS             2362..2721
                     /gene="frdD"
                     /locus_tag="ACHOT6_005210"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFP9950195.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            2784..3917
                     /locus_tag="ACHOT6_005211"
     CDS             2784..3917
                     /locus_tag="ACHOT6_005211"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFP9950196.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            4006..4539
                     /gene="blc"
                     /locus_tag="ACHOT6_005212"
     CDS             4006..4539
                     /gene="blc"
                     /locus_tag="ACHOT6_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFP9950197.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(4536..4853)
                     /gene="sugE"
                     /locus_tag="ACHOT6_005213"
     CDS             complement(4536..4853)
                     /gene="sugE"
                     /locus_tag="ACHOT6_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFP9950198.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5035..5181)
                     /gene="ecnB"
                     /locus_tag="ACHOT6_005214"
     CDS             complement(5035..5181)
                     /gene="ecnB"
                     /locus_tag="ACHOT6_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFP9950199.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5292..5417)
                     /gene="ecnA"
                     /locus_tag="ACHOT6_005215"
     CDS             complement(5292..5417)
                     /gene="ecnA"
                     /locus_tag="ACHOT6_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFP9950200.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(5469..6035)
                     /gene="efp"
                     /locus_tag="ACHOT6_005216"
     CDS             complement(5469..6035)
                     /gene="efp"
                     /locus_tag="ACHOT6_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFP9950201.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            6077..7105
                     /gene="epmB"
                     /locus_tag="ACHOT6_005217"
     CDS             6077..7105
                     /gene="epmB"
                     /locus_tag="ACHOT6_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFP9950202.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1783 a         1861 c         1881 g         1645 t
ORIGIN      
        1 cgcgtttatc gttacaacac caacggcggc atcgttaccg gtgacggtat gggtatggcg
       61 ctaagccacg gcgttccgct gcgtgacatg gaattcgttc agtatcaccc aaccggtctg
      121 ccaggttccg gtatcctgat gaccgaaggc tgccgcggtg aaggcggtat tctggtcaac
      181 aaaaatggct accgttatct gcaagattac ggcatgggcc cggaaactcc gctgggcgag
      241 ccgaaaaaca aatatatgga actgggtcca cgcgacaaag tttctcaggc cttctggcac
      301 gaatggcgta aaggcaacac catctccacg ccgcgtggcg atgtggttta tctcgacttg
      361 cgtcacctcg gcgagaaaaa actgcatgaa cgtctgccgt tcatctgcga actggcgaaa
      421 gcgtacgttg gcgtcgatcc ggttaaagaa ccgattccgg tacgtccgac cgcacactac
      481 accatgggcg gtatcgaaac cgatcagaac tgtgaaaccc gcattaaagg tctgttcgcc
      541 gtgggtgaat gttcctctgt tggtctgcac ggtgcaaacc gtctgggctc caactccctg
      601 gcggaactgg tggtcttcgg ccgtctggcc ggtgaacaag cgacagagcg tgcagcaact
      661 gccggtaatg gcaacgaagc ggcaattgaa gcgcaggcag ctggcgttga acaacgtctg
      721 aaagatctgg ttaaccagga tggcggcgaa aactgggcga agatccgcga cgaaatgggc
      781 ctggcaatgg aagaaggttg cggtatctac cgtacgccgg aactgatgca gaaaaccatc
      841 gacaagctgg cagagctgca ggaacgcttc aagcgcgtgc gcatcaccga cacctccagc
      901 gtgttcaaca ccgacctgct ctacaccatt gaactgggcc acggtctgaa cgttgctgaa
      961 tgtatggcgc actccgcaat ggcacgtaaa gagtcccgcg gcgcacacca gcgtctggac
     1021 gaaggttgca ccgagcgtga cgacgtcaac ttcctcaaac acaccctcgc cttccgcgat
     1081 gctgatggca cgactcgcct ggagtacagc gacgtgaaga ttactacgct gccgccagct
     1141 aaacgcgttt acggtggcga agcggatgca gccgataagg cggaagcagc caataagaag
     1201 gagaaggcga atggctgaga tgaaaaacct gaaaattgag gtggtgcgct ataacccgga
     1261 agtcgatacc gcaccgcata gcgcattcta tgaagtgcct tatgacgcaa ctacctcatt
     1321 actggatgcg ctgggctaca tcaaagacaa cctggcaccg gacctgagtt accgctggtc
     1381 ctgccgtatg gcgatttgtg gttcctgcgg catgatggtt aacaacgtgc caaaactggc
     1441 atgtaaaacc ttcctgcgtg attacaccga cggtatgaag gttgaagcgt tagctaactt
     1501 cccgattgaa cgcgatctgg tggtcgatat gacccacttc atcgaaagtc tggaagcgat
     1561 caaaccgtac atcatcggca actcccgcac cgcggatcag ggtactaaca tccagacccc
     1621 ggcgcagatg gcaaagtatc accagttctc cggttgcatc aactgtggtc tgtgctacgc
     1681 cgcgtgcccg cagtttggcc tgaacccaga gttcatcggt ccggctgcca ttacgctggc
     1741 gcatcgttat aacgaagata gccgcgacca cggtaagaag gagcgtatgg cgcagttgaa
     1801 cagccagaac ggcgtatgga gctgtacttt cgtgggctat tgctccgaag tctgcccgaa
     1861 acacgtcgat ccggctgcgg ccattcagca gggcaaagta gaaagttcga aagactttct
     1921 tatcgcgacc ctgaaaccac gctaaggagt gcaacatgac gactaaacgt aaaccgtatg
     1981 tacggccaat gacgtccacc tggtggaaaa aattgccgtt ttatcgcttt tacatgctgc
     2041 gcgaaggcac ggcggttccg gctgtgtggt tcagcattga actgattttc gggctgtttg
     2101 ccctgaaaaa tggcccggaa gcctgggcgg gattcgtcga ctttttacaa aacccggtta
     2161 tcgtgatcat taacctgatc actctggcgg cagctctgct gcacaccaaa acctggtttg
     2221 aactggcacc gaaagcggcc aatatcattg taaaagacga aaaaatggga ccagagccaa
     2281 ttatcaaaag tctctgggcg gtaactgtgg ttgccaccat cgtaatcctg tttgttgccc
     2341 tgtactggta aggagcctga gatgattaat ccaaatccaa agcgttctga cgaaccggta
     2401 ttctggggcc tcttcggggc cggtggtatg tggagcgcaa ttattgcgcc ggtgatgatc
     2461 ctgctggtgg gtattctgct gccactgggg ctgtttccgg gtgatgcgct gagctacgag
     2521 cgcgttctgg cgttcgcgca gagcttcatt ggtcgcgtat tcctgttcct gatgatcgtt
     2581 ctgccgctgt ggtgtggttt acaccgtatg caccacgcga tgcacgatct gaaaattcac
     2641 gtgcctgcgg gcaaatgggt tttctacggt ctggctgcta tcctgacagt tgtcacgctg
     2701 attggtatcg ttacaatcta acgtatcgcc aatgtaaatc cggcccgcct atggcgggcc
     2761 gttttgtatg gaaaccagac cttatgttca aaacgacgct ctgcgcctta ttaattaccg
     2821 cctcttgctc cacatttgct gcccctcaac aaatcaacga tattgtgcat cgcacaatta
     2881 ccccgcttat agagcaacaa aagatccccg gtatggcggt ggcggtaatt tatcagggta
     2941 aaccttatta ctttacctgg ggctatgcgg acatcgccaa aaagcagccc gtcacacagc
     3001 aaacgttgtt tgagttaggt tcggtcagca aaacatttac gggcgtgctt ggtggcgagg
     3061 ctattgctcg aggggaaatc aagttaagcg atcccacaac aaaatactgg cctgaactta
     3121 ccgctaaaca gtggaatggg atcacactat tacatctcgc aacctacact gctggcggcc
     3181 tgccattgca ggtgccggat gaggtgaaat cctcaagcga cttgctgcgc ttctatcaaa
     3241 actggcagcc tgcatgggcg ccaggaacac aacgtctgta tgccaactcc agtatcggtt
     3301 tgttcggcgc actggctgtg aagccgtctg gtttgagttt tgagcaggcg atgcaaacac
     3361 gtgtcttcca gccactcaaa ctcaaccata cgtggattaa tgtaccgccc gcagaagaaa
     3421 agaattacgc ctggggatat cgcgaaggca aggcagttca tgtttcgcca ggggcgttag
     3481 atgctgaaac ttatggtgtg aagtcgacca ttgaagatat ggcctgctgg gtacgaagca
     3541 atatgaatcc ccgtgatatc aacgacaaaa cacttcagca agggatacaa ctggcacaat
     3601 ctcgctactg gcaaaccggc gatatgtatc agggcctggg ctgggaaatg ctggactggc
     3661 cggtaaatcc tgacagcatc attaacggca gtggcaataa aattgcactg gcagcacacc
     3721 ccgtaaaagc gattacgccc ccaactcctg cagtacgcgc atcatgggta cataaaacag
     3781 gggcaaccgg cggatttggt agctatgtcg cgtttattcc agaaaaagag ctgggtatag
     3841 tgatgctggc taacaaaaac tatcccaatc cagcgagagt cgccgccgcc tggcagattc
     3901 tcaacgctct acagtaaaat ttcatcgggt ccgaattttc ggaccttttc tgcgcttttc
     3961 cttgctgtca tctacactta gaaaaaaacc agtaaggaaa catttatgcg cctgctccct
     4021 ctcgttgccg cagcgacagc tgcatttctg gtcgttgcct gcagttctcc tacgccgccg
     4081 cgtggcgtaa cagtagtgaa taatttcgac gccaaacgtt atctcggtac ctggtatgag
     4141 attgcccgtt ttgatcaccg ttttgaacgt ggactggaaa aagtcaccgc aacatacagc
     4201 ctgcgtgatg acggcggcct gaatgtcatt aataaaggct ataaccctga cagaggaatg
     4261 tggcagcaga gtgaagggaa agcgtacttt accggcgcac caactcgcgc tgcgctgaaa
     4321 gtgtcattct ttggtccttt ctatggcggt tataacgtta ttgcactcga tcgggaatac
     4381 cgccatgcgc tggtttgcgg cccggaccgc gactacctgt ggatactctc ccgcacgcca
     4441 accatttctg acgaagtgaa acaggagatg ctggcagtcg cgacccggga agggtttgat
     4501 gtcagtaagt ttatttgggt acagcagccc ggtagttagt gagtgctgag tttcagacca
     4561 ataatcccca atacgattaa cgccagactc gccaggcgca tcgggttagc ggactcaccg
     4621 agcagcacaa tgccggtgat agccgcgccg actgcgccaa tacccgtcca cacggcataa
     4681 gccgtcccta ctggtaacga tttcatcgcc caggcaagta gcgccatact gacaatcatc
     4741 gccgtcacgg taataacact cggcgtcaaa cgactaaagc cgtgggtata tttcaggcca
     4801 acggcccata ccacttccag cagaccagca ataactaaga taatccagga catatcaggc
     4861 tccggagaat tggggccgtc cccggtgaaa agaggcgttt gcaggtcgtc ctgcaaagct
     4921 tatggttgaa cggatatttt gccaggtggc aggggaattt caactattac cttagtgact
     4981 atcgcagaaa aaacaaacgg cacgacatac ctgtcgtacc gttattgctt attgctattg
     5041 ctgcgctttc gttgctgcgc cagaaatcgc gttaccgcca tcagaaatgt cttcaccaac
     5101 gccacgcgtg gtgttgcagg cagttaatac tgttgaaagc accagaacag aaaagatcgc
     5161 tgcaattgtc ttcttcacca taacgtcttc cttttaggta atgttattta tgtttgccta
     5221 tagcaaatta ataatagaca acatcgccaa aaatgacgga aatctgatga tttttcggaa
     5281 gagaagaaaa attagctggc agcgcgagag atggagttgc cgagatgttt gatgtcttcg
     5341 ccgaaaccgc gagcggtgtt acagcccgtg agcagcgtgc tggcaagcaa aacaagaacg
     5401 ataaggcgtt tcatcatctc tgacctgaat aagtgatggt gcagcctgca ggccgcacca
     5461 caaccgcatt acttcacgcg agagacgtat tcaccagagc gggtatccac tttgatgact
     5521 tcgccgattt gtacgaacag cggaacctta accacagcgc cagtagacag ggtagccggt
     5581 ttgccgccag tacctgcggt atcacctttc aggcccggat cggtatcaac gatttccagt
     5641 tcaacgaagt tcggcggagt aacggagatc ggctgaccat tccacagagt tacgatacac
     5701 tctgcctgat ccagcagcca tttagcgttg tcgccaattg cttttgcatc agcagacagc
     5761 tgctcgaaag tttcgttgtt catgaagtgc cagaactcac cgtcgttgta caggtaagtc
     5821 aggttcatat cgacaacatc agcgccttca gcggaatcag tagatttgaa ggttttttct
     5881 acgcgagtac cggtcagcag acgacgcagt ttaacgcgag caaatgcctg gcctttaccc
     5941 ggttttacga attcactcgc ttcaaccgcg taaggttcgc cgtctaacat gattttaaga
     6001 ccagcacgaa aatcgttgct atagtacgtt gccataaggc cctctgaaat ttgttaattg
     6061 gtagctaagc cacaaaatgg cgcatattgt aaccctaaat accccatcca gagaagattg
     6121 gttaacgcaa cttgccgatg ttgtgaccga tcctgatgaa cttctgcgtc ttttgaatat
     6181 agacgcggac gaaaaactgt tggccggacg cagcgccaaa aagctgtttg ccctgcgtgt
     6241 gccccgctca tttatcgatc gcatggagaa aggcaatccg gacgatcctc ttttgcgtca
     6301 ggtacttacc tcgcaagatg agtttgtcgt cgcgtccgga ttctccaccg acccgctgga
     6361 agaacagcac agcgtagtgc ctggtttgtt gcataaatac cacaaccggg cgcttttgct
     6421 ggtcaaaggc ggctgcgcgg taaattgccg ctattgcttc cgtcgccact tcccctatgc
     6481 cgaaaatcag ggcaacaagc gtaactggca gactgcactt gagtatgttg ctgcgcatcc
     6541 ggaactggac gagatgattt tctccggcgg cgatccgctg atggcgaaag atcacgagct
     6601 ggactggttg ctcacacaac tggaagccat cccgcatatc aaacgtctgc ggattcacag
     6661 ccgtctgccg attgtgatcc cggcacgtat caccgaggcg ctggttgaac gctttgcccg
     6721 ttctacgctg caaatcttgc tggtgaatca catcaatcat gccaatgagg tagatgaaac
     6781 gttccgtcag gcgatggcta aattacgccg tgtcggtgtc accctgctta accagagcgt
     6841 tctgttacgt ggtgtgaacg ataacgcaca aacgttggca aacctgagta atgcgttgtt
     6901 cgatgccggc gtgatgccgt attacctgca tgtgctagat aaagtacagg gcgcggcgca
     6961 ttttatggtg agtgatgacg aagcacggca gattatgcgt gagttgctga cattggtgtc
     7021 gggatatctg gtgccgaaac tggcgcgaga aattggcggc gaacccagca aaacgccgct
     7081 ggatctccag ctacgccagc agtaactgat taaacaggga agttaaacat tagcctgatg
     7141 cgctacgctt atcaggcctt gtattatccc
//
LOCUS       DAZKIV010000511         6393 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.guided.202, whole genome shotgun sequence.
ACCESSION   DAZKIV010000511 DAZKIV010000000
VERSION     DAZKIV010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.guided.202"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            35..652
                     /locus_tag="ACHOT6_005218"
                     /pseudo
     CDS             35..652
                     /locus_tag="ACHOT6_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            649..1263
                     /gene="prfH"
                     /locus_tag="ACHOT6_005219"
     CDS             649..1263
                     /gene="prfH"
                     /locus_tag="ACHOT6_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFP9950203.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHOT6_005220"
     CDS             complement(1320..2777)
                     /gene="pepD"
                     /locus_tag="ACHOT6_005220"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFP9950204.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3038..3496
                     /gene="gpt"
                     /locus_tag="ACHOT6_005221"
     CDS             3038..3496
                     /gene="gpt"
                     /locus_tag="ACHOT6_005221"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFP9950205.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3588..4832
                     /gene="frsA"
                     /locus_tag="ACHOT6_005222"
     CDS             3588..4832
                     /gene="frsA"
                     /locus_tag="ACHOT6_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFP9950206.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRVC"
     gene            4890..5291
                     /gene="crl"
                     /locus_tag="ACHOT6_005223"
     CDS             4890..5291
                     /gene="crl"
                     /locus_tag="ACHOT6_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFP9950207.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHOT6_005224"
     CDS             complement(5330..6385)
                     /gene="phoE"
                     /locus_tag="ACHOT6_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFP9950208.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
BASE COUNT         1561 a         1569 c         1647 g         1616 t
ORIGIN      
        1 caccccatta taaggggtac gaataaagta acgggggcag tctattttac agcggcatat
       61 tgcctcgttc tcgcatcatg gtttgcctca aggcagtgac gacgcgctac ggtatattgc
      121 ggaacatgac gatgcgctgg cgtttgcgtg tattaatcgc cagatgatcg ctttgcgcat
      181 aatgcaacaa gttaaggcca cgggaagtcc ggttctggat gtggcgcata actttgttag
      241 cgcgtgtcaa atcggcgatc aacagggctg gttgcatcgt aaaggtgcca cgccggatga
      301 caacggtctg gtgattattc ccggttcacg tggtgattac tcctggattg tacagcctgt
      361 tgcgaacgaa aaaacgctcc actcgctggc gcatggggca gggcgtaaat gggggcgcac
      421 cgagtgtaaa gggcgtctgg cagcgaaata cacggcgacg caactctcgc gtactgaact
      481 tggcagtcgg gtaatttgtc gcgataaaca actcatcttt gaagaagcgc cacaagccta
      541 taaatcggct gaaagcgtgg tgcaatgtct ggtgcaggcc ggtttaatta ttcctgtcgc
      601 gcggctgcgt ccggtgctaa cgctcaaaaa cagtggaggg aaaaaaggat gatcttgcta
      661 caactctcct cagcgcaggg gccggaagaa tgttgtctcg cagtgaaaaa ggcgctggac
      721 aggctaatta aagaagctgc ccgacaagac gtcgcggtaa cggtgttgga aacagaaacg
      781 ggccgctact ctgacacgct gcgttcggcg ctgatttctc tggatggcga taacgcatgg
      841 gcattaagcg aaagctggtg tggcactatt cagtggattt gtccgagtcc gtatcggcct
      901 catcatgggc gcaaaaactg gtttctgggc attgggcgtt ttaccgctga tgagcaggaa
      961 caatcggatg caatccgtta tgagacgttg cgttcgtcgg ggccgggcgg tcaacatgtc
     1021 aataaaaccg actcggcggt acgcgccacg catttggcat ccggtattag cgtgaaggtt
     1081 cagtcagagc gtagtcagca tgctaacaag cggctggcac gattgctgat tgcctggaag
     1141 ctggagcagc agcaacagga aaatagcgcg gtgctgaaat cgcagcggcg aatgttccat
     1201 caccagattg aacgtggcaa cccgagacgg acatttacag ggatggcttt tatcgaagga
     1261 taatgaagga tgaaactgcc ggaaggcgat taaacgccat ccggcagcaa atcaaataat
     1321 tacttcgccg gaatttcttt cagcagttca gtcagcagcg tccagtaatg acctacgctt
     1381 tcgatgtgaa cttgctcatc cggagagtgt ggaccggtga tagttggccc gatagaaacc
     1441 atgtccattt ccggatacgg ttttttgaac agaccacatt ccaggcccgc gtggataatc
     1501 tggatgttcg gtgttttgtt gaacagacgc tgataggttt cacgtaccag atgcatcacc
     1561 ggagaattag cgtccggctg ccagccagga tatgcgcctt tcgcttcggt tttcgcgcca
     1621 gctagtttac ccagcgaatc cagcatgctc accacgtagt ctttaccgct gtcgatcagt
     1681 gaacggatca ggcagtgaat ttctacgtta ttgtcagtca tggtcaccac accgacgttc
     1741 agggaggttt cgaccacacc ttttgccacg tcggaattgc ggatcacacc gttcggggtg
     1801 gcgttcagca gacgaataaa ggtatcgcga gatttcgcaa tcagggcagc tttatcgttc
     1861 gctacagagt ccagcaacaa ggccagattt ttctctttct ctgccagctc gtttttcagg
     1921 atctcctgat aggtattcac cagagatttc aggacgtcga ctttatcagc tgcgacagca
     1981 atggtcgcaa aggcttcacg cgggatggcg ttacgcagtg tgccgccgtt gaaatcgata
     2041 aggcgcagat ccagttcttc cgcatgaccc gccaggaagc gcaccagtag tttgttggca
     2101 ttacccaggc caacgtggat ttcaccgccg gagtgaccgc ctttcagacc ttttaaggtt
     2161 aacttgaagg tttcaaaacc agctggaacc gcttcacgat ctaaatgcag gttggaggtg
     2221 aagtcgatac cccccgcaca acccatgtag atttcacctt cttcttcgga gtcggtgtta
     2281 atcagaatat cagcctgcaa ccagttgctc tgtaagccaa aagcaccgtc cataccggct
     2341 tcttcggtca tggtcagcag cacttccagc ggaccgtgaa ccacgttttc gtcagccaga
     2401 accgccagtg cagaggccat accaatgccg ttatccgcac ccagcgtggt gccgcgcgct
     2461 ttaacccatt cgccatcaat ataaggctgg ataggatctt tcgtgaagtc atgcacggtg
     2521 tcgttatttt tctgcggcac catatcgagg tgggcctgta agacgaccgg tttacgattt
     2581 tccatacctg cggtagcagg tttacgaatc aggatattac ctacctgatc gcgttcgaca
     2641 tggaaacctt tctcttttgc ccaaccaaca atgtattcag cgagttgctc ttcatgatag
     2701 gacgggtgag gaatagaaca gattttggca aaaatatccc acagcggctg tggagataat
     2761 tgagacagtt cagacacgtt aagtctcctt gtcgatcacc cgcaaaacag tattgcaggt
     2821 cacagggtta gcagaaaatg ttgtcaacac aagacaggct tgcgagatat gtttgagaat
     2881 accactttat ctcgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     2941 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     3001 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     3061 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     3121 atggaaaggc attattgccg taagccgtgg cggtctggta ccgggtgcgt tactggcgcg
     3181 tgaactgggt attcgtcatg tcgataccgt ttgtatttcc agctacgatc acgacaacca
     3241 gcgagagctt aaagtgctga aacgcgcaga aggcgatggc gaaggcttca tagttattga
     3301 tgacctggtg gataccggtg gtactgctgt tgcgattcgt gaaatgtatc caaaagcgca
     3361 ctttgtcacc atcttcgcaa aaccggctgg tcgtccgctg gttgatgact atgttgttga
     3421 tatcccgcaa gatacctgga tcgaacagcc gtgggatatg ggcgtcgtat tcgtcccgcc
     3481 aatctccggt cgctaatctt ttcaacgcct ggcactgccg ggcgttgttc tttttaactt
     3541 caggcaggtt acaatagttt ccagtaagta ttctggaggc tgcatccatg acacaggcaa
     3601 acctgagcga aaccctgttc aaaccccgct ttaaacatcc tgaaacctcg acgctagtcc
     3661 gccgctttaa tcacggcgca caaccgcctg tgcagtcggc tcttgatggt aaaaccatcc
     3721 ctcactggta tcgcatgatt aaccgtctga tgtggatctg gcgcggtatt gacccacgtg
     3781 aaatcctcga cgtccaggca cgtattgtga tgagcgatgc tgaacgtacc gacgatgatt
     3841 tatacgatac tgtgattggc taccgtggtg gcaactggat ttatgagtgg gccacccagg
     3901 cgatggtgtg gcaacaaaaa gcctgtgcgg aagaagatcc gcaactcagt ggtcgacact
     3961 ggctgcatgc ggctacgttg tacaacattg ccgcctatcc tcatctgaaa ggagatgacc
     4021 tggccgagca agcgcaggct ttatcaaacc gcgcctatga agaggccgcc cagcgtctgc
     4081 cgggcacgat gcggcagatg gagtttaccg tacccggcgg tgcgcccatc accggctttt
     4141 tgcatatgcc gaaaggcgat ggcccgttcc cgacagtatt aatgtgtggt ggtctggatg
     4201 cgatgcagac ggactattac agcctgtatg aacgttattt tgcgccgcgc ggcattgcga
     4261 tgctgactat tgatatgccg tcggtgggct tttcttcaaa atggaagctc acccaggact
     4321 ccagcctgtt gcatcagcac gtcttaaagg cgctgcctaa cgtaccgtgg gtggatcaca
     4381 ctcgcgtcgc ggcctttggt ttccgtttcg gcgctaacgt tgccgtgcgt ctggcatatc
     4441 ttgaatcgcc gcgtctgaaa gcggttgcct gtcttggtcc ggtagttcat accctgttga
     4501 gtgattttaa gtgccagcaa caggtgccgg aaatgtatct tgacgttctg gcgagtcgtt
     4561 tggggatgca tgatgcttcc gacgaagcgt tacgcgtgga gctgaatcgc tattcattaa
     4621 aagtgcaagg attgctggga cgtcgctgcc caacgccaat gttatcaggc tactggaaga
     4681 acgatccgtt cagcccggaa gaggactcac gcttaatcac ctcatcatct gctgacggta
     4741 aattattaga gatcccattt aacccggtgt atcggaattt tgacaaaggt cttcaggaaa
     4801 tcaccggctg gatcgaaaaa cgcgtgtgtt aaaaatttgc taaattttgc caatttggta
     4861 aaacagttgc atcacaacag gagatagcaa tgacgttacc gagtggacac ccgaagagca
     4921 gattgatcaa aaaatttacc gcactaggcc cgtatattcg tgaaggtaag tgcgaagata
     4981 atcgattctt tttcgattgt ctggctgtat gcgtcaacgt gaaaccggca ccggaagtgc
     5041 gtgaattctg gggctggtgg atggagctgg aagcgcagga atcccgtttt acctacagct
     5101 accagtttgg tctgttcgat aaagcaggcg actggaagag tgttccggta aaagatactg
     5161 aagtggttga acgactggag cacaccctgc gtgagtttca cgagaagctg cgtgaactgc
     5221 tgacgacgct gaatctgaag ctggaaccgg cggatgattt tcgtgacgag ccggtgaagt
     5281 taacggcgtg agtgaaatgt gccggatgca tcacatccgg caatatctat taaaactgat
     5341 acgtcatccc aatcgcgaca atatcatcat tattaatatt caatttgtta tcgctatcca
     5401 gttggttgat tttataatca acaaatgctg acatattttt gttgaaataa tacgtagcac
     5461 cgacgtcgat ataattaacc agatcttcac caccgatgcc ctcaatatct ttccctttcg
     5521 ataagacata acccagcgat ggacgcagac caaagtcaaa ctggtattga gcgaccgctt
     5581 caaagttctg tgtcttattg gcaaagccgc cagttattgg cgtcattttg cgagtttcag
     5641 aatagaaagt tgccagataa atattattgg catcgtattt cagacctgtt gcccatgctt
     5701 ctgcacgctt gccagtgcca cggctttgca ggttctgctc gttggtgcga tctgagttgg
     5761 tataggcacc actaatggcg aaatcgctgc cgccaaagtc atatgtcaat gacgtgccga
     5821 agccatcgcc gttttgcttt ttaacgtcgc ggttttcgtt tttcccttga tattgcaggg
     5881 ttaagttcag gccatcgata acgccgaaga agtcggtgtt ccgatacgtc gccagaccgc
     5941 tggcgcgttt ggtcataaag ttgtcggtct gcgccgagga atcgccacca aattccggga
     6001 acatatcggt ccaggcttcc acgtcataca acgcgcccag gttacgacca taatcgaaag
     6061 aacccaaatc tttatatttc aacccggcaa aagcgagacg cgttttttgc tgtgcagtat
     6121 cactctccgc tttatttccg gcaaactccg cttcccaccg accataacca gtcagttgat
     6181 cgttaatttg tgtttcacct ttaaaaccaa aacggatata actctggtcg ccatctttac
     6241 tggcgttatc actcatataa tgcatggctt taactttgcc atagacatcc agtttattac
     6301 cgtctttatt atatatttct gcagcctgta cagatgcaga tgccacaatg cccatcacca
     6361 ctaatgccag agtgctcttt ttcattttca ttc
//
LOCUS       DAZKIV010000512         1506 bp    DNA     linear   BCT 23-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2104H73666
            SAMN43267446-rid23077113.guided.347, whole genome shotgun sequence.
ACCESSION   DAZKIV010000512 DAZKIV010000000
VERSION     DAZKIV010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43267446
            Sequence Read Archive: SRR30307176
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002350494.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002350494.1
            Genome Coverage       :: 104x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 06:42:30
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,226
            CDSs (total)                      :: 5,138
            Genes (coding)                    :: 4,913
            CDSs (with protein)               :: 4,913
            Genes (RNA)                       :: 88
            rRNAs                             :: 1, 1, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 91 of 225
            Pseudo Genes (incomplete)         :: 115 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 32 of 225
            Pseudo Genes (short protein)      :: 1 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43267446-rid23077113.guided.347"
                     /serotype="O182:H25"
                     /isolate="2104H73666"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2021-04"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..979
                     /gene="stxA1a"
                     /locus_tag="ACHOT6_005225"
     CDS             32..979
                     /gene="stxA1a"
                     /locus_tag="ACHOT6_005225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFP9950209.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            989..1258
                     /gene="stxB1a"
                     /locus_tag="ACHOT6_005226"
     CDS             989..1258
                     /gene="stxB1a"
                     /locus_tag="ACHOT6_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFP9950210.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          410 a          257 c          376 g          463 t
ORIGIN      
        1 atcgtatggt gctcaaggag tattgtgtaa tatgaaaata attattttta gagtgctaac
       61 ttttttcttt gttatctttt cagttaatgt ggtggcgaag gaatttacct tagacttctc
      121 gactgcaaag acgtatgtag attcgctgaa tgtcattcgc tctgcaatag gtactccatt
      181 acagactatt tcatcaggag gtacgtcttt actgatgatt gatagtggca caggggataa
      241 tttgtttgca gttgatgtca gagggataga tccagaggaa gggcggttta ataatctacg
      301 gcttattgtt gaacgaaata atttatatgt gacaggattt gttaacagga caaataatgt
      361 tttttatcgc tttgctgatt tttcacatgt tacctttcca ggtacaacag cggttacatt
      421 gtctggtgac agtagctata ccacgttaca gcgtgttgca gggatcagtc gtacggggat
      481 gcagataaat cgccattcgt tgactacttc ttatctggat ttaatgtcgc atagtggaac
      541 ctcactgacg cagtctgtgg caagagcgat gttacggttt gttactgtga cagctgaagc
      601 tttacgtttt cggcaaatac agaggggatt tcgtacaaca ctggatgatc tcagtgggcg
      661 ttcttatgta atgactgctg aagatgttga tcttacattg aactggggaa ggttgagtag
      721 cgtcctgcct gactatcatg gacaagactc tgttcgtgta ggaagaattt cttttggaag
      781 cattaatgca attctgggaa gcgtggcatt aatactgaat tgtcatcatc atgcatcgcg
      841 agttgccaga atggcatctg atgagtttcc ttctatgtgt ccggcagatg gaagagtccg
      901 tgggattacg cacaataaaa tattgtggga ttcatccact ctgggggcaa ttctgatgcg
      961 cagaactatt agcagttgag ggggtaaaat gaaaaaaaca ttattaatag ctgcatcgct
     1021 ttcatttttt tcagcaagtg cgctggcgac gcctgattgt gtaactggaa aggtggagta
     1081 tacaaaatat aatgatgacg atacctttac agttaaagtg ggtgataaag aattatttac
     1141 caacagatgg aatcttcagt ctcttcttct cagtgcgcaa attacgggga tgactgtaac
     1201 cattaaaact aatgcctgtc ataatggagg gggattcagc gaagttattt ttcgttgact
     1261 cagaatagct cagtgaaaat agcaggcgga gattcataaa tgttaaatac atctcaattc
     1321 agtcagttgt tgccggtctg ataatagatg tgttagaaaa tttctgcatg gtgaatcccc
     1381 ctgtgcggag gggcgactgg tgaacggtat gatctctttg atgatcgtaa gcgagaatac
     1441 gcgggtttgg tggcaccagg ccgaactcac cgggaggcac ccggcatcat gctgtataca
     1501 gagatt
//
